LOCUS       DAETPZ010000001       141941 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.001, whole genome shotgun sequence.
ACCESSION   DAETPZ010000001 DAETPZ010000000
VERSION     DAETPZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 141941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 141941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..141941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.001"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="K0W59_000001"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="K0W59_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBI3021205.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="K0W59_000002"
     CDS             complement(1672..2730)
                     /locus_tag="K0W59_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI3021206.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="K0W59_000003"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="K0W59_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="K0W59_000004"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="K0W59_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HBI3021207.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="K0W59_000005"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="K0W59_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HBI3021208.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="K0W59_000006"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="K0W59_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HBI3021209.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="K0W59_000007"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="K0W59_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HBI3021210.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="K0W59_000008"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="K0W59_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HBI3021211.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLANPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRHRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13099)
                     /gene="yfdE"
                     /locus_tag="K0W59_000009"
                     /pseudo
     CDS             complement(11955..13099)
                     /gene="yfdE"
                     /locus_tag="K0W59_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
     gene            complement(13173..14117)
                     /gene="yfdV"
                     /locus_tag="K0W59_000010"
     CDS             complement(13173..14117)
                     /gene="yfdV"
                     /locus_tag="K0W59_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HBI3021212.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14187..15881)
                     /gene="oxc"
                     /locus_tag="K0W59_000011"
     CDS             complement(14187..15881)
                     /gene="oxc"
                     /locus_tag="K0W59_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HBI3021213.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIHYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRNILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15935..17185)
                     /gene="frc"
                     /locus_tag="K0W59_000012"
     CDS             complement(15935..17185)
                     /gene="frc"
                     /locus_tag="K0W59_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HBI3021214.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17698..18321)
                     /locus_tag="K0W59_000013"
     CDS             complement(17698..18321)
                     /locus_tag="K0W59_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HBI3021215.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18618..18893
                     /locus_tag="K0W59_000014"
     CDS             18618..18893
                     /locus_tag="K0W59_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3021216.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18970..19212)
                     /locus_tag="K0W59_000015"
     CDS             complement(18970..19212)
                     /locus_tag="K0W59_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HBI3021217.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19565..20485
                     /gene="lpxP"
                     /locus_tag="K0W59_000016"
     CDS             19565..20485
                     /gene="lpxP"
                     /locus_tag="K0W59_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HBI3021218.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20840..20911
                     /gene="ypdK"
                     /locus_tag="K0W59_000017"
     CDS             20840..20911
                     /gene="ypdK"
                     /locus_tag="K0W59_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HBI3021219.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20976..22214)
                     /gene="alaC"
                     /locus_tag="K0W59_000018"
     CDS             complement(20976..22214)
                     /gene="alaC"
                     /locus_tag="K0W59_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HBI3021220.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22591..24288
                     /gene="ypdA"
                     /locus_tag="K0W59_000019"
     CDS             22591..24288
                     /gene="ypdA"
                     /locus_tag="K0W59_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HBI3021221.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            24303..25037
                     /gene="ypdB"
                     /locus_tag="K0W59_000020"
     CDS             24303..25037
                     /gene="ypdB"
                     /locus_tag="K0W59_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HBI3021222.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            25050..25907
                     /locus_tag="K0W59_000021"
     CDS             25050..25907
                     /locus_tag="K0W59_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3021223.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(25910..28411)
                     /gene="ptsP"
                     /locus_tag="K0W59_000022"
     CDS             complement(25910..28411)
                     /gene="ptsP"
                     /locus_tag="K0W59_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBI3021224.1"
                     /translation="MLTIQFLCPLPMPNGLHARPARELKEQCSQWQSEITFINHRQNA
                     KADAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAEL
                     TAHPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHS
                     LATLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNM
                     EQVCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTP
                     SQFLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQ
                     CGVLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEA
                     PGAFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGD
                     KSISYLNVPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLD
                     QILWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSND
                     MTQYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLP
                     LLLGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPE
                     EDVRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTG
                     VGFGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVF
                     SQLARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28436..29473)
                     /gene="ypdE"
                     /locus_tag="K0W59_000023"
     CDS             complement(28436..29473)
                     /gene="ypdE"
                     /locus_tag="K0W59_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBI3021225.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKILGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29473..30556)
                     /gene="ypdF"
                     /locus_tag="K0W59_000024"
                     /pseudo
     CDS             complement(29473..30556)
                     /gene="ypdF"
                     /locus_tag="K0W59_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30571..31818)
                     /locus_tag="K0W59_000025"
     CDS             complement(30571..31818)
                     /locus_tag="K0W59_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBI3021226.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGENGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31840..32166)
                     /locus_tag="K0W59_000026"
     CDS             complement(31840..32166)
                     /locus_tag="K0W59_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HBI3021227.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32385..33350)
                     /gene="glk"
                     /locus_tag="K0W59_000027"
     CDS             complement(32385..33350)
                     /gene="glk"
                     /locus_tag="K0W59_000027"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HBI3021228.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33554..34810
                     /locus_tag="K0W59_000028"
     CDS             33554..34810
                     /locus_tag="K0W59_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HBI3021229.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAIMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34925..35251
                     /locus_tag="K0W59_000029"
     CDS             34925..35251
                     /locus_tag="K0W59_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBI3021230.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35298..36536)
                     /locus_tag="K0W59_000030"
     CDS             complement(35298..36536)
                     /locus_tag="K0W59_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBI3021231.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36872..38074
                     /gene="nupC"
                     /locus_tag="K0W59_000031"
     CDS             36872..38074
                     /gene="nupC"
                     /locus_tag="K0W59_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HBI3021232.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38124..40313)
                     /locus_tag="K0W59_000032"
                     /pseudo
     CDS             complement(38124..40313)
                     /locus_tag="K0W59_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40521..40596)
                     /locus_tag="K0W59_000033"
     tRNA            complement(40521..40596)
                     /locus_tag="K0W59_000033"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40561..40563),aa:Ala,seq:ggc)
     gene            complement(40636..40711)
                     /locus_tag="K0W59_000034"
     tRNA            complement(40636..40711)
                     /locus_tag="K0W59_000034"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40676..40678),aa:Ala,seq:ggc)
     gene            40932..41291
                     /locus_tag="K0W59_000035"
     CDS             40932..41291
                     /locus_tag="K0W59_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBI3021233.1"
                     /translation="MSKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41293..41685
                     /locus_tag="K0W59_000036"
     CDS             41293..41685
                     /locus_tag="K0W59_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBI3021234.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41737..43152)
                     /gene="gltX"
                     /locus_tag="K0W59_000037"
     CDS             complement(41737..43152)
                     /gene="gltX"
                     /locus_tag="K0W59_000037"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBI3021235.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43411..43486
                     /locus_tag="K0W59_000038"
     tRNA            43411..43486
                     /locus_tag="K0W59_000038"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43444..43446,aa:Val,seq:tac)
     gene            43531..43606
                     /locus_tag="K0W59_000039"
     tRNA            43531..43606
                     /locus_tag="K0W59_000039"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43564..43566,aa:Val,seq:tac)
     gene            43611..43686
                     /locus_tag="K0W59_000040"
     tRNA            43611..43686
                     /locus_tag="K0W59_000040"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43644..43646,aa:Lys,seq:ttt)
     gene            43717..43842
                     /locus_tag="K0W59_000041"
     ncRNA           43717..43842
                     /ncRNA_class="other"
                     /locus_tag="K0W59_000041"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43962..44303
                     /locus_tag="K0W59_000042"
     CDS             43962..44303
                     /locus_tag="K0W59_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HBI3021236.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44294..45220)
                     /locus_tag="K0W59_000043"
     CDS             complement(44294..45220)
                     /locus_tag="K0W59_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3021237.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45310..46308
                     /locus_tag="K0W59_000044"
     CDS             45310..46308
                     /locus_tag="K0W59_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HBI3021238.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46305..46523)
                     /locus_tag="K0W59_000045"
     CDS             complement(46305..46523)
                     /locus_tag="K0W59_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HBI3021239.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46525..48540)
                     /gene="ligA"
                     /locus_tag="K0W59_000046"
     CDS             complement(46525..48540)
                     /gene="ligA"
                     /locus_tag="K0W59_000046"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBI3021240.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGSTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQELMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48611..49498)
                     /gene="zipA"
                     /locus_tag="K0W59_000047"
     CDS             complement(48611..49498)
                     /gene="zipA"
                     /locus_tag="K0W59_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HBI3021241.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPVSPQVAPAPQPVHSAPQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEA
                     VIIMNVAAHHGSELNGELLLNSIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKP
                     GTFDPEMKDFTTPGVTIFMQVPSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMT
                     PQKLREYQDIIREVKDANA"
     gene            49728..50489
                     /gene="cysZ"
                     /locus_tag="K0W59_000048"
     CDS             49728..50489
                     /gene="cysZ"
                     /locus_tag="K0W59_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HBI3021242.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50674..51645
                     /gene="cysK"
                     /locus_tag="K0W59_000049"
     CDS             50674..51645
                     /gene="cysK"
                     /locus_tag="K0W59_000049"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBI3021243.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            52029..52286
                     /gene="ptsH"
                     /locus_tag="K0W59_000050"
     CDS             52029..52286
                     /gene="ptsH"
                     /locus_tag="K0W59_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HBI3021244.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52331..54058
                     /gene="ptsI"
                     /locus_tag="K0W59_000051"
     CDS             52331..54058
                     /gene="ptsI"
                     /locus_tag="K0W59_000051"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HBI3021245.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVAQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            54099..54608
                     /gene="crr"
                     /locus_tag="K0W59_000052"
     CDS             54099..54608
                     /gene="crr"
                     /locus_tag="K0W59_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBI3021246.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54650..55501)
                     /gene="pdxK"
                     /locus_tag="K0W59_000053"
                     /gene_synonym="thiJ"
     CDS             complement(54650..55501)
                     /gene="pdxK"
                     /locus_tag="K0W59_000053"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HBI3021247.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55606..55980
                     /locus_tag="K0W59_000054"
     CDS             55606..55980
                     /locus_tag="K0W59_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HBI3021248.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            56013..56736
                     /locus_tag="K0W59_000055"
                     /pseudo
     CDS             56013..56736
                     /locus_tag="K0W59_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56940..57851)
                     /gene="cysM"
                     /locus_tag="K0W59_000056"
     CDS             complement(56940..57851)
                     /gene="cysM"
                     /locus_tag="K0W59_000056"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HBI3021249.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57985..59082)
                     /gene="cysA"
                     /locus_tag="K0W59_000057"
     CDS             complement(57985..59082)
                     /gene="cysA"
                     /locus_tag="K0W59_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HBI3021250.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(59072..59947)
                     /gene="cysW"
                     /locus_tag="K0W59_000058"
     CDS             complement(59072..59947)
                     /gene="cysW"
                     /locus_tag="K0W59_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HBI3021251.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59947..60780)
                     /gene="cysT"
                     /locus_tag="K0W59_000059"
     CDS             complement(59947..60780)
                     /gene="cysT"
                     /locus_tag="K0W59_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HBI3021252.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60780..61796)
                     /gene="cysP"
                     /locus_tag="K0W59_000060"
     CDS             complement(60780..61796)
                     /gene="cysP"
                     /locus_tag="K0W59_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HBI3021253.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDEAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(62100..62891)
                     /gene="ucpA"
                     /locus_tag="K0W59_000061"
     CDS             complement(62100..62891)
                     /gene="ucpA"
                     /locus_tag="K0W59_000061"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HBI3021254.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(63078..63878)
                     /gene="murR"
                     /locus_tag="K0W59_000062"
     CDS             complement(63078..63878)
                     /gene="murR"
                     /locus_tag="K0W59_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HBI3021255.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            64042..64938
                     /gene="murQ"
                     /locus_tag="K0W59_000063"
     CDS             64042..64938
                     /gene="murQ"
                     /locus_tag="K0W59_000063"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBI3021256.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64942..66366
                     /gene="murP"
                     /locus_tag="K0W59_000064"
     CDS             64942..66366
                     /gene="murP"
                     /locus_tag="K0W59_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HBI3021257.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAARACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66546..67445)
                     /gene="yfeX"
                     /locus_tag="K0W59_000065"
     CDS             complement(66546..67445)
                     /gene="yfeX"
                     /locus_tag="K0W59_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HBI3021258.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMVL"
     gene            complement(67541..68116)
                     /locus_tag="K0W59_000066"
     CDS             complement(67541..68116)
                     /locus_tag="K0W59_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HBI3021259.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68177..68626)
                     /locus_tag="K0W59_000067"
     CDS             complement(68177..68626)
                     /locus_tag="K0W59_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HBI3021260.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68613..69038)
                     /locus_tag="K0W59_000068"
     CDS             complement(68613..69038)
                     /locus_tag="K0W59_000068"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HBI3021261.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLSPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69252..70121
                     /gene="amiA"
                     /locus_tag="K0W59_000069"
     CDS             69252..70121
                     /gene="amiA"
                     /locus_tag="K0W59_000069"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HBI3021262.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            70125..71024
                     /gene="hemF"
                     /locus_tag="K0W59_000070"
     CDS             70125..71024
                     /gene="hemF"
                     /locus_tag="K0W59_000070"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HBI3021263.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(71030..72083)
                     /gene="eutR"
                     /locus_tag="K0W59_000071"
                     /pseudo
     CDS             complement(71030..72083)
                     /gene="eutR"
                     /locus_tag="K0W59_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(72168..72629)
                     /gene="eutK"
                     /locus_tag="K0W59_000072"
     CDS             complement(72168..72629)
                     /gene="eutK"
                     /locus_tag="K0W59_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HBI3021264.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGT"
     gene            complement(72642..73301)
                     /gene="eutL"
                     /locus_tag="K0W59_000073"
     CDS             complement(72642..73301)
                     /gene="eutL"
                     /locus_tag="K0W59_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBI3021265.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(73311..74198)
                     /gene="eutC"
                     /locus_tag="K0W59_000074"
     CDS             complement(73311..74198)
                     /gene="eutC"
                     /locus_tag="K0W59_000074"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HBI3021266.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(74219..75580)
                     /gene="eutB"
                     /locus_tag="K0W59_000075"
     CDS             complement(74219..75580)
                     /gene="eutB"
                     /locus_tag="K0W59_000075"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HBI3021267.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAIAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDGCYTNHADSDQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTSTVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            75769..76974
                     /locus_tag="K0W59_000076"
     CDS             75769..76974
                     /locus_tag="K0W59_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBI3021268.1"
                     /translation="MSKTPLTAKAIDAAQPKDKQYKLTASLTPGLFLLVHPNGSKYWR
                     FRYWIDKKERLQAIGVYPLISLKEARKRATESRLLIAQGIDPMDLARKEKAIDALNMA
                     AGFKTVAEDWLATRVGGWSESYTKQVKSALEKDVYPVLGKRSIVDITARDVLALLQKK
                     ERTAPEQARKLRQRIGEIFKFAVITELVTRNPVADLDTALKARRPGHNAWIPISEIPA
                     FYKALERAGSVQIQAAIRLLILTALRTAELRLCRWEWVNLEDATITLPAEVMKARRPH
                     VVPLSRQAVELLQDQFTRSGYSVFVFPGRFMDKPLSASAILKALERIGYKSIATGHGW
                     RTTFSTALNESGRYNPDWIEIQLAHVPKGIRGVYNQAAYLKMRRQMLQDYADAIDSIL
                     ADGINPLEL"
     gene            77489..78205
                     /locus_tag="K0W59_000077"
     CDS             77489..78205
                     /locus_tag="K0W59_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3021269.1"
                     /translation="MRIDQILSPANEEPALANAEPAQEQEQEQEQEQEQNKAAAIAEF
                     KELQRQQITAQQEECRHPLAMPKPVAKANTDKPTSWFNEEAEEDLRFDSKEEQLPEER
                     EQVETEDQQNDYRNLPAVNNAMAYLFKSGLFHKLKGDELKLYTWMVSKCRYRGNDKYE
                     IWYSRTKMAKALGKSVDTIDRHLEALRKLGLIQEMRQALRGKCGKTYRLRIPSKGTAE
                     EASMTAQQHEREAKVSYLVH"
     gene            79256..80008
                     /locus_tag="K0W59_000078"
     CDS             79256..80008
                     /locus_tag="K0W59_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3021270.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIYTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCRE"
     gene            80768..81163
                     /locus_tag="K0W59_000079"
     CDS             80768..81163
                     /locus_tag="K0W59_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBI3021271.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHKGKFKVTLTYQKACYIYTYGAYDETLYEVHHVNLDKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            81181..81444
                     /locus_tag="K0W59_000080"
     CDS             81181..81444
                     /locus_tag="K0W59_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3021272.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            81467..82009
                     /locus_tag="K0W59_000081"
     CDS             81467..82009
                     /locus_tag="K0W59_000081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3021273.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            82474..82680
                     /locus_tag="K0W59_000082"
     CDS             82474..82680
                     /locus_tag="K0W59_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3021274.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            82992..83477
                     /locus_tag="K0W59_000083"
     CDS             82992..83477
                     /locus_tag="K0W59_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBI3021275.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            83458..83736
                     /locus_tag="K0W59_000084"
     CDS             83458..83736
                     /locus_tag="K0W59_000084"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3021276.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNIREYKNV"
     gene            83729..85189
                     /locus_tag="K0W59_000085"
     CDS             83729..85189
                     /locus_tag="K0W59_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI3021277.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            85223..86032
                     /gene="murI"
                     /locus_tag="K0W59_000086"
     CDS             85223..86032
                     /gene="murI"
                     /locus_tag="K0W59_000086"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBI3021278.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEKYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(86151..86845)
                     /locus_tag="K0W59_000087"
     CDS             complement(join(86151..86599,86599..86845))
                     /locus_tag="K0W59_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3021279.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(87369..88742)
                     /gene="eutA"
                     /locus_tag="K0W59_000088"
     CDS             complement(87369..88742)
                     /gene="eutA"
                     /locus_tag="K0W59_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBI3021280.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVSDLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVCATVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(88717..89196)
                     /gene="eutP"
                     /locus_tag="K0W59_000089"
     CDS             complement(88717..89196)
                     /gene="eutP"
                     /locus_tag="K0W59_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HBI3021281.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIVVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(89209..89544)
                     /gene="eutS"
                     /locus_tag="K0W59_000090"
     CDS             complement(89209..89544)
                     /gene="eutS"
                     /locus_tag="K0W59_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HBI3021282.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(89837..92116)
                     /gene="maeB"
                     /locus_tag="K0W59_000091"
     CDS             complement(89837..92116)
                     /gene="maeB"
                     /locus_tag="K0W59_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HBI3021283.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFVADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            92405..93355
                     /gene="tal"
                     /locus_tag="K0W59_000092"
     CDS             92405..93355
                     /gene="tal"
                     /locus_tag="K0W59_000092"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBI3021284.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPLDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            93375..95378
                     /gene="tkt"
                     /locus_tag="K0W59_000093"
     CDS             93375..95378
                     /gene="tkt"
                     /locus_tag="K0W59_000093"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBI3021285.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATCLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(95481..96548)
                     /locus_tag="K0W59_000094"
     CDS             complement(95481..96548)
                     /locus_tag="K0W59_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004985426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HBI3021286.1"
                     /translation="MNFWGRTVHALSHFPSLFFALLPTSLVWAAPAQRAFSDWQVTCN
                     NQNFCVARNTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGE
                     PLALSGDKWRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFI
                     DAQQKRVGSETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWR
                     MNGLRCSLDPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRL
                     RLPFNNGQETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYA
                     AEPTCDNWHGPDARPTLWITR"
     gene            complement(96649..97224)
                     /gene="nudK"
                     /locus_tag="K0W59_000095"
     CDS             complement(96649..97224)
                     /gene="nudK"
                     /locus_tag="K0W59_000095"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HBI3021287.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(97292..99260)
                     /gene="aegA"
                     /locus_tag="K0W59_000096"
                     /pseudo
     CDS             complement(97292..99260)
                     /gene="aegA"
                     /locus_tag="K0W59_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
     gene            99466..101183
                     /gene="narQ"
                     /locus_tag="K0W59_000097"
                     /pseudo
     CDS             99466..101183
                     /gene="narQ"
                     /locus_tag="K0W59_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            101385..104498
                     /gene="acrD"
                     /locus_tag="K0W59_000098"
     CDS             101385..104498
                     /gene="acrD"
                     /locus_tag="K0W59_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HBI3021288.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFIHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSADFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNSYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGTQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(104597..104656)
                     /gene="ypfM"
                     /locus_tag="K0W59_000099"
     CDS             complement(104597..104656)
                     /gene="ypfM"
                     /locus_tag="K0W59_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HBI3021289.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            105037..105393
                     /locus_tag="K0W59_000100"
     CDS             105037..105393
                     /locus_tag="K0W59_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HBI3021290.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLYAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            105397..106524
                     /gene="dapE"
                     /locus_tag="K0W59_000101"
     CDS             105397..106524
                     /gene="dapE"
                     /locus_tag="K0W59_000101"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HBI3021291.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            106552..106752
                     /locus_tag="K0W59_000102"
     CDS             106552..106752
                     /locus_tag="K0W59_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HBI3021292.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(106862..107560)
                     /gene="ypfH"
                     /locus_tag="K0W59_000103"
     CDS             complement(106862..107560)
                     /gene="ypfH"
                     /locus_tag="K0W59_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBI3021293.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(107634..109649)
                     /gene="tmcA"
                     /locus_tag="K0W59_000104"
     CDS             complement(107634..109649)
                     /gene="tmcA"
                     /locus_tag="K0W59_000104"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HBI3021294.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(109664..110527)
                     /locus_tag="K0W59_000105"
     CDS             complement(109664..110527)
                     /locus_tag="K0W59_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HBI3021295.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRCSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(110695..111408)
                     /gene="purC"
                     /locus_tag="K0W59_000106"
     CDS             complement(110695..111408)
                     /gene="purC"
                     /locus_tag="K0W59_000106"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBI3021296.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVAHRLGVQLD"
     gene            complement(111621..112655)
                     /gene="bamC"
                     /locus_tag="K0W59_000107"
     CDS             complement(111621..112655)
                     /gene="bamC"
                     /locus_tag="K0W59_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HBI3021297.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPASLEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(112672..113550)
                     /gene="dapA"
                     /locus_tag="K0W59_000108"
     CDS             complement(112672..113550)
                     /gene="dapA"
                     /locus_tag="K0W59_000108"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBI3021298.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            113696..114268
                     /locus_tag="K0W59_000109"
     CDS             113696..114268
                     /locus_tag="K0W59_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HBI3021299.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            114268..114738
                     /gene="bcp"
                     /locus_tag="K0W59_000110"
     CDS             114268..114738
                     /gene="bcp"
                     /locus_tag="K0W59_000110"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBI3021300.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            114991..115608
                     /gene="hyfA"
                     /locus_tag="K0W59_000111"
     CDS             114991..115608
                     /gene="hyfA"
                     /locus_tag="K0W59_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HBI3021301.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            115608..117625
                     /gene="hyfB"
                     /locus_tag="K0W59_000112"
                     /pseudo
     CDS             115608..117625
                     /gene="hyfB"
                     /locus_tag="K0W59_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            117636..118583
                     /gene="hyfC"
                     /locus_tag="K0W59_000113"
     CDS             117636..118583
                     /gene="hyfC"
                     /locus_tag="K0W59_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HBI3021302.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLLTLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGQGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            118600..120039
                     /gene="hyfD"
                     /locus_tag="K0W59_000114"
     CDS             118600..120039
                     /gene="hyfD"
                     /locus_tag="K0W59_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HBI3021303.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISDYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            120051..120701
                     /gene="hyfE"
                     /locus_tag="K0W59_000115"
                     /pseudo
     CDS             120051..120701
                     /gene="hyfE"
                     /locus_tag="K0W59_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
     gene            120706..122286
                     /gene="hyfF"
                     /locus_tag="K0W59_000116"
     CDS             120706..122286
                     /gene="hyfF"
                     /locus_tag="K0W59_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HBI3021304.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            122276..123990
                     /gene="hyfG"
                     /locus_tag="K0W59_000117"
                     /pseudo
     CDS             122276..123990
                     /gene="hyfG"
                     /locus_tag="K0W59_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            124000..124545
                     /gene="hyfH"
                     /locus_tag="K0W59_000118"
     CDS             124000..124545
                     /gene="hyfH"
                     /locus_tag="K0W59_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HBI3021305.1"
                     /translation="MLKLLKTIMRAGTPTVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            124542..125300
                     /gene="hyfI"
                     /locus_tag="K0W59_000119"
     CDS             124542..125300
                     /gene="hyfI"
                     /locus_tag="K0W59_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HBI3021306.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            125293..125706
                     /gene="hyfJ"
                     /locus_tag="K0W59_000120"
     CDS             125293..125706
                     /gene="hyfJ"
                     /locus_tag="K0W59_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HBI3021307.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            125736..127748
                     /gene="hyfR"
                     /locus_tag="K0W59_000121"
                     /pseudo
     CDS             125736..127748
                     /gene="hyfR"
                     /locus_tag="K0W59_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            127770..128618
                     /locus_tag="K0W59_000122"
                     /pseudo
     CDS             127770..128618
                     /locus_tag="K0W59_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(128656..129717)
                     /locus_tag="K0W59_000123"
     CDS             complement(128656..129717)
                     /locus_tag="K0W59_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBI3021308.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            129930..131393
                     /gene="bepA"
                     /locus_tag="K0W59_000124"
     CDS             129930..131393
                     /gene="bepA"
                     /locus_tag="K0W59_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HBI3021309.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVLQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLEQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            131414..131773
                     /gene="arsC"
                     /locus_tag="K0W59_000125"
     CDS             131414..131773
                     /gene="arsC"
                     /locus_tag="K0W59_000125"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HBI3021310.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(131906..132652)
                     /gene="hda"
                     /locus_tag="K0W59_000126"
     CDS             complement(131906..132652)
                     /gene="hda"
                     /locus_tag="K0W59_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HBI3021311.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(132702..133991)
                     /gene="uraA"
                     /locus_tag="K0W59_000127"
     CDS             complement(132702..133991)
                     /gene="uraA"
                     /locus_tag="K0W59_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBI3021312.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(134077..134703)
                     /gene="upp"
                     /locus_tag="K0W59_000128"
     CDS             complement(134077..134703)
                     /gene="upp"
                     /locus_tag="K0W59_000128"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBI3021313.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            135028..136065
                     /gene="purM"
                     /locus_tag="K0W59_000129"
     CDS             135028..136065
                     /gene="purM"
                     /locus_tag="K0W59_000129"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBI3021314.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            136065..136703
                     /gene="purN"
                     /locus_tag="K0W59_000130"
     CDS             136065..136703
                     /gene="purN"
                     /locus_tag="K0W59_000130"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBI3021315.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQSYAADE"
     gene            136875..138941
                     /gene="ppk1"
                     /locus_tag="K0W59_000131"
     CDS             136875..138941
                     /gene="ppk1"
                     /locus_tag="K0W59_000131"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HBI3021316.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFGHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            138946..140487
                     /gene="ppx"
                     /locus_tag="K0W59_000132"
     CDS             138946..140487
                     /gene="ppx"
                     /locus_tag="K0W59_000132"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HBI3021317.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLLRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(140526..141662)
                     /locus_tag="K0W59_000133"
     CDS             complement(140526..141662)
                     /locus_tag="K0W59_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBI3021318.1"
                     /translation="MSVKLRLPQYKQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTES
                     HQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIV
                     TNAPENMQRCGAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILL
                     RMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSP
                     TSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAI
                     DDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAE
                     YVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
     gene            141701..141941
                     /locus_tag="K0W59_000134"
                     /pseudo
     CDS             141701..141941
                     /locus_tag="K0W59_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT        35458 a        36573 c        34940 g        34970 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 gcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccaa
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat gcggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaagctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggcaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcatcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aaacgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgattctt gtaacctttc ccatgaaaac cccagttttt
    12781 ccattgtacc taggcgaaaa ttctcagcta atacatcagc ttgtttgagc atatttataa
    12841 atatactttt atcgtgatca ttctttaaat caagaaccac actctctttg ccatgattaa
    12901 taaaactgta atagagtgac tgtccatcca catagggacc aaatgtgcgg gtatcatcac
    12961 catgacctgg tggctcaact ttaattaccc ttgcgcccat attataaaga agttgagttc
    13021 cgaaaggtcc attaaggaca tgtgtcatat cgataactaa taagccttca aacggccctt
    13081 tgctttcatt atttgtcatt tgcttatgcc ttgaaaaaag ccgaaggtga aaagacactt
    13141 cggcatttat gtaaaatcaa taacatgatt aattatgaaa ccagtcgact gacataaatc
    13201 cacaagggag ccgtgacgac aaagcccagt acgctcacag ccaatgacgc tgtaccagta
    13261 cgggtgtaga cattaaaccg gctggcaata atgattccgg agaatgccgg cggtaatgcg
    13321 cctgccagta ccatcatctg caaatgttcg ctgttcaaat gacatgccat accaacgaga
    13381 agcagcgcca gtggcatcag aatcaacttc aggaaggtgt tataagcaat ttcggcactg
    13441 aactcgaatt tatgtgcagc cagagtcaac cccgcagcga ataccgctac ccctgagtta
    13501 gccttcgcaa tcagattaaa ggttgggtcc catgctgccg gaatttttac cccaaccagc
    13561 accaggatcg ttgccagaac aggtgcccat actactggct cctttgccgc agaaattaat
    13621 gcgctcagat tactattctt cttaccatcc gctcctgagg aaggattcag caaatacaga
    13681 ccaataggaa tagtaattgc gttaacaata atagaaataa ttgctaccac taaacctgtt
    13741 gatacggaat caccataaat aggatcgaga actgcaaacc ccaggaatcc aatggtaggt
    13801 gaacccgcaa ttaatgcaca tacagctgct tctgcatggg tacgtttaaa aaatttgtag
    13861 caaccaaacc aggagaaaaa gaaacatccg acaataacca caagtgatac aagggtcaga
    13921 cgagtgtccg caaaaatcat ttccctgttt gcccgggtaa tagaaacaaa tagagccgca
    13981 ggaagagcgt agtttaatac cagtttatta aatgcgcggg cttgatcttc tgaaaaagtt
    14041 tctcgtctgc cgctaaaata acccaacagc atgattacga taataggcaa taaatcgcca
    14101 ataaaaaatg ttagcataat agtatttccg ataagggtcc cctcaccatc gcataatgag
    14161 ttggcaagag gagacgatgt cagtatttaa ttaccagcga cttgttttgg gttaagtttg
    14221 gtaatatggc cactttcagt tcctgctgca gggtcgataa ccacattaat aatggtcggt
    14281 ttgcgcgact gaataccggt ggttaaagca tgacgaagtt catctgtcgt ggtgacgtta
    14341 tagccaacgc cacgaaacgc atccattaat ttgtcatacc ttgcatggtg caacagatcc
    14401 gttggtgatg gtgcaccagc gccactgaga tcaacaccgt ctcctctgta gatgccgcca
    14461 ttattaaaaa taacgatcgt caccggtagg ttatatcgac aaatcgtttc aatttccatc
    14521 ccactgaaac caaaagcact atcaccctca atggcgacaa ccggagaacc agaagtcacg
    14581 ctagcaccga tggcatagcc cataccgatg cccatgacac cccaggtacc acaatccaga
    14641 cgacgacgtg gtttatacat atcaataata tttcgtgcat tatccagggt atttgcacct
    14701 tcattaacta aataaatatc ctggttctcg cgcaatacgt cgcgcacagc acttaatgca
    14761 ttaaagtaat ttaatggttg agtatctgta cttaattttt catgcatttt ttgtgcattt
    14821 tgctgcttgt ggatatttaa aatattgcgc cataccagtg gagtcgtaaa tgtgttttgt
    14881 ttcagttctg ccagcatacc ttgcatactg gatgcaatat caccaacgac tggcacagca
    14941 atggggcggt tgctgtcaat ttcctgcggt tcaatatcca gttgaataaa ctgtgtatct
    15001 gccgcccatc cttttttacc gtgtgccaat aaccaattca gtcgtgcacc aacaagcatg
    15061 acaacgtcag catttgccag ggcaaacgaa cgcgcagctg ccgcagaaag tggatgcgta
    15121 tcttcaagga tccctttcgc catagacatt ggcaggaatg gaatctgagc actttcaata
    15181 aattcacgaa gctgttcatc agcttgtgaa tacgccgcgc ctttgccaag gataattaat
    15241 ggccgttcag cttttgctaa aagcgaaatt gcgctagtga ctgacttcgg gcatggcaat
    15301 aatgctggcg acggattttc aactttaaca atcgtggtta acgcttcgtc tttttccatc
    15361 gtcgcggcca ggacatttgc tggcaaatca agataaactc cgccagggcg acccgataca
    15421 gagacccgga tagcgcgtgc caatgcaatg ccaagatcct gcggctgatt aacgcgaaat
    15481 gctgctttgg catacggttt tgccgcattc atttggtcca gctcttcata atcaccttgc
    15541 tgtaggtcga cgatcgcgcg gtcgctggag ccgctaatca tgatcatcgg aaaaccattt
    15601 accgttgcgt tggccaatgc ggtcaaacca ttgaggaatc ctggcgcaga aactgtcagg
    15661 cagatccccg gtttttgggt aagaaaaccg cttgccgcag cggcatagcc tgccgactgc
    15721 tcatgacgaa aaccaatata atgaatgcct tccgcctggg catggcgtgc catatccgtc
    15781 acaggaatac ctacaacacc ataaatagtg tcaatattat tctgttttaa tgcttcaacg
    15841 atgatatgca taccatctgt catttgaagt tgatctgaca tgattaataa cctcgaaatt
    15901 tttataaatt gggagccaga agcccccgtt aatatcagat ggcgtggttt tgcttcattg
    15961 cagcaatttc atcgtcgcta taacccagct cctgcaatac agcagcggta tgttcaccta
    16021 atagcggcgc agctttaata tccggcgtaa aggcagagaa tttcattgga caaccaacgg
    16081 tcagatattt tccacgcaac ggttgttcca cttcaacaac actgccactt tggcgcaaag
    16141 agggatcaag tgaaatttct ttcatactta aaaccggtgc acaaggaata tcaaactgag
    16201 tcaaataggc caccgcttca tgtttatcaa tagtgacagt gtatttttcg atttcagcaa
    16261 aaatatcgaa aatatgtggc tgtcgggcat gggctgtact gtatgccgga tcggtaatcc
    16321 attctggttt gccgatggct ttacaggtgt tttcccagtt ttgctcctga atagtgaaat
    16381 aaatataggc gttaggatcg gtttcccagc ctttacattt caggatccag ccaggctgac
    16441 cgccaccacc tgcattacca ccgcggggaa ctgcatcacc aaatgtacca ttcggatact
    16501 gcgggtattc ttccagatac cccaatttat cgagacgctg ctggtcacgt aatttcacgc
    16561 ggcaaaggtt caatacggca tcctgcattg acatggtgac tcgttgccca cgccccgttt
    16621 tttcgcgatg cagcaaagca gcaagtaaac cgatcagcaa atgcattccg gtgttgctgt
    16681 cacccaacgc tgcagcgctt accagcggcg gaccatccca aaaacccgta gtggatgccg
    16741 cgccacccgc tgcctgagca acgttttcat aggcttttac attcacataa ggcgaacact
    16801 catcaaaccc tttgatcgaa ccaaaaatca gacgtggatt gatttcttga atatgctccc
    16861 aggtgaagcc catgtgatca atggcccctg gatgaaagtt ctcgactaag atatcagctt
    16921 cgcggatcag cttttccatt acctctttgc cttccgctgt tttggtattt aactcaatag
    16981 aacgtttgtt actgttaagc atggtgaagt aaagcgcatc gatatcagga atatctcgca
    17041 gttggtgacg cgttacgtca ccaacgccgg gacgttcaat tttaataacg tcagcgccaa
    17101 accaggccag catttgagta caagatgggc cagattgcac accggtgaaa tcgagaactt
    17161 taattccttg aagtggagtt gacatagcaa tacctattag ttaattaaat atgtgttaat
    17221 ttagcgttta tttaaggcat aacgccctct ataaataatc cgcaaaatat cttcgtagcg
    17281 gcactcgata tttttaaatt cctgtatcaa gtactgaaaa ttatttcatc gctacttcca
    17341 attcctttct gcatggtaag tagtgaggac gaaaagaaga atatgataaa tattgattcg
    17401 tggtaatcag agagagtcgc gacatttgtg atgttattct tacaactgta agaagcgccg
    17461 gtgatttaca agtaagagta tgattacgct ttttaaagcg taccataaag cataacataa
    17521 taaatgattt gtgatgacac gggattattg cggtttactt tgaggaaaat aaaatgcgtg
    17581 tttatagaac cgcgagcgcc aaataatttt atgaccacaa cctgtcgtat tgtggtcata
    17641 aaaaattgtc ggctggaggg agttaaatcc tttacctcaa tatactgcct gtcagcatta
    17701 gttcacaaac agtgcttcgc tatcgacgat gattccatct tccgcacctt taagcgccag
    17761 gtttgcctcg tagtattgct ttttatcaag cagtttctgc gcatcagcaa gtgcattacg
    17821 agtctgttta agcggcatca gatattggtt ttccattacg ccaacaccgg ccaggcgaag
    17881 ctcttccatt gcgccctttt tatccccttt cgccattttt tcattggcaa tttttatcgc
    17941 ggcttctttt tcgggtgttg ctacataact ctcagaaatt ccgacagatg cattaatgac
    18001 aatatattgg tcatcattga gattggtctt tttacctggc ttagcgaact ttgcccattc
    18061 ggtactgtca tctgacagca aagcggaagc ttcattggtc agttcttttg ccttttccgg
    18121 atcgccatga aacagagcca ggcgcgctac ctgcacgtca cgcatcgcat ataaaccttg
    18181 ttcagagata cgctctgctg ctgccgtttt ctgggtttgc tgagtttgct gttgtgccgc
    18241 taccggtgca ttatcagcag cccatacagt tgaagatgcc agaattgctg tgaccatcgt
    18301 agccataatt aaacgtttca tacttacctc attctttaac agttttagaa aacgccgcca
    18361 tcatattggg tgagcatgaa atgagaaatc agagagcaac ggtcattttg aaagggttat
    18421 cttacaattg tagttattat cgcaaagcgg catttagtct tacagttgta ggaatatgcc
    18481 tcctgtcgca gagaactatt cttatatacc acaaagagat gtcatttatt atattgatgt
    18541 aaatattgat taattatatg taaatacagc cataacatcg tatggttctc agtgaaccat
    18601 tttcaggata actttctatg aaagtaaact taatactttt cagcttattt ttattggtct
    18661 ctattatggc atgcaatgtt tttgcatttt ccatttctgg tggtgtaagt gagaggagct
    18721 ataaagagac tgaaaaaaca tcagcgatga cgaccacaca ctctacaaaa cttcagccat
    18781 cacaggcgat tttgtttaag atgagagaag atgtgccacc attaaacctc acagaagaaa
    18841 tcacgcctac ttatccgaca aaggcgaatt atcttattca tcctgtgaga tagcgtctgt
    18901 cacatggcag gtgaacaatc atgtctgatg ctgatgtgaa cacctgccca aatgagccat
    18961 cctgacccac tatcgcctgc cataccccac cataaagcga atcatcaagc cacaagccgg
    19021 ggcaagaaac ataaatatcc ccgtcaccag ttcattaccg ccagttaacc acatggtgaa
    19081 gtaataaatg agtgcagcga ggatcaccat gccgaagaaa gaagagaccg ccgataccac
    19141 gttcagtacc tgaccgatgt agccgcttac gcagacaata cacagggcga gaaacatcca
    19201 taaataaatc atagctcaac ctcctgccgc gctaaaaaac ctgtaacttc agtcacttta
    19261 atagtaagac aatccttaac ctctggcaat ttttgctaag catcaatgaa aacagcacgt
    19321 taaatgcaac gctattgcgt gcggaaaatt ttttactttg cacgattaat aatcagtagc
    19381 tgaaagcagt cagccagtga taaagtagag ccaccgcatt catgctcctc gtggttatgt
    19441 cctgactagt ctttacactc tttacaggaa ccattgtcgt acatgatggc ccaaccaatt
    19501 gagtgtaccg ctgtcgacat ggaatcttct gcgatacaac aattcgtatc tacagaaggt
    19561 aactatgttt ccacaatgca aattttcccg cgagtttcta catcctcgct actggctcac
    19621 atggtttggg cttggtgtac tctggctttg ggtacagctt ccttatcctg ttctctgctt
    19681 tctcggcacg cgtattggcg caatggcgcg accattcctg aaacgtcgtg aatctatcgc
    19741 ccgtaaaaac ctggaacttt gtttcccgca gcattctgcg gaagaacgcg agaagatgat
    19801 tgccgaaaac tttcgttcac tcggcatggc gctggtagaa accggcatgg catggttctg
    19861 gcccgacagt cgcgtacgta aatggtttga tgttgaaggg ttggataacc ttaaacgcgc
    19921 acaaatgcaa aatcgcggcg taatggttgt cggcgtccat tttatgtcgc tggaactggg
    19981 cggccgcgtg atgggactgt gccaaccaat gatggctacc tatcgtccac ataataatca
    20041 gctgatggaa tgggtgcaga cccgtgggcg catgcgctct aacaaagcga tgatcggcag
    20101 aaataatctg cgcggcattg tcggtgcact gaagaaaggt gaagcggtat ggtttgctcc
    20161 cgatcaggat tatggtcgta aaggcagctc cttcgcgccg ttctttgcgg tggaaaatgt
    20221 cgccacaacc aatggcacct atgttctctc ccgtctctct ggcgcagcca tgttgaccgt
    20281 aacgatggta agaaaagcgg attacagcgg atatcgtttg ttcatcaccc cagagatgga
    20341 aggctacccg acagatgaaa atcaagccgc tgcttatatg aacaagatta tcgagaaaga
    20401 gatcatgcgc gcaccggagc agtacctctg gatccaccgt cgctttaaaa cgcgcccggt
    20461 gggagaatcg tcgttgtaca tttaacaaac tgctcttact ctttccctcc tcaaatcggc
    20521 cataataacc tcatggtttt tatggctttt ttcttaagtt ttttccttcg ctgaatatct
    20581 aattattacc gtcagaaatt aaactgtcgc cgatatgaga cacttgtaag ttactaaaag
    20641 taaataagaa aatgcctctt gtctcgtttc agttttaggc gtacattagc gccgtctcga
    20701 gcagtttagc tcagaattct ggtgtgatgc ccacggtaaa atacagggct tttaaggtcg
    20761 ctttgcccgg tgtcaactca ctgtatcagg tgtaatgaag tcattcaggc gtaacagtaa
    20821 ttacgcggag agatgtaaag tgaaatattt ctttatgggc atttctttta tggtcatcgt
    20881 ttgggccggt acttttgccc tgatgatcta aaagcaaact gtgcaatgaa aaacaggagc
    20941 catcaggctc ctgtttgcat ttctgacgac ggtgtttatt ccgcgttttc gtgaatatgt
    21001 ttgctgctgg cgggtagtaa accgtcggca cggaacatcg ctttaatccc tctgatggcc
    21061 tgacgaatac ggtcgcggtt ttcaatcagt gcaaagcgaa catgcgtatc accatagtcg
    21121 ccaaagccaa tccccggcga gacacaaacc ttcgcttcgt taaggagctt cttggcaaat
    21181 tccagcgatc ccatggccgc atatggttcc gggattttcg cccagacata catcgaagcc
    21241 ttcggcattt cgaccatcca gcccgcttca tgcagccctt taaccaatac atcacgacgg
    21301 cgtttgtact gttcggcaat gtcgcgcacg cactgttgat cgccctcgag tgccgcaatc
    21361 gccgccacct gcaacggcgt aaaagtgccg taatcgtgat agcttttgat tcgcgccagg
    21421 gcactgacca gcgttttgtt gccaaccata aagccgatac gccagcccgc catgttgtag
    21481 cttttcgaca gcgtaaagaa ctcgaccgcc acatcgcgtg cacccggcac ctgcataatt
    21541 gacggcgctt tccagccatc gtagacgata tcggcatagg ccaggtcatg gaccaccagc
    21601 acatcgtagc gtttcgccag cgccacaacc ttttcgaaga actccagttc cacgcattgc
    21661 gcggttgggt tcgacgggaa gccgaggatc atcatcttcg gtttcggata actttcacga
    21721 atggcgcgct ccagttcgtt gaagaaatcg acaccttcca ccagcggcac tgagcgtacc
    21781 tgcgccccgg caatcaccgc gccataaata tggatcgggt aacttggatt cggcaccagc
    21841 accgtgtcac catgatccag cgtcgccagc atcagatgcg ccaggccctc tttcgaacca
    21901 atagtgacga tggcttctga ttccgggtcg atttcaacgt cgtagcgatc ctgataccag
    21961 cgggaaatgg cgcgacgtaa ccgcggaatg ccgcgtgaag tggagtaacc atgcgtgtcc
    22021 gggcgctggg ccacagtaca taatttttcg acgatatgcg gcggagtcgc accgtccggg
    22081 ttacccatgc tgaaatcgat aatatcttcg ccgcgccgac gcgcagccat tttcagttca
    22141 gcggtgatgt taaaaacgta gggcgggaga cgatcaatgc gcgtaaagcg acgttcaggg
    22201 cgagtgtcag ccataatttc ctcagattaa cgttagcgcc cggaccgtcc gagcgacgct
    22261 gccacaatgg tggctgtttt gaaaatagcc tgattaattt ctgtctgtcc agcgttttag
    22321 aacattttta tcaatggatt taaatgagga actccacttc aaaaacagga aatttttcta
    22381 cgctaacaag aaaattacca ccaattttat taacattata atttcaataa atttaccagc
    22441 cagaagtgca ttatcaagag atacattgct aatacaccat ttatctttca atcccctttt
    22501 aaatttatgg tttttcctca aaaacggcct gctggtcatc agccttcagg ttacctatca
    22561 tagaggttta atccttattc agagtcaccc gtgcacgaaa tattcaacat gctgctggcg
    22621 gtcttcgatc gggcagcgtt aatgcttatc tgcctgttct ttctcatccg tatccgcctg
    22681 tttcgcgaac tgttgcacaa gtcggcgcac tccccaaaag aattgctcgc cgttaccgcc
    22741 attttctcgc tgttcgccct gttcagcacc tggtccggcg ttcccgtaga aggctcgctg
    22801 gtgaacgtac gtattatcgc ggtgatgtcc ggcgggattc tgtttggccc gtgggtaggc
    22861 atcattaccg gcgtgattgc gggtattcac cggtatttaa ttgatatcgg cggcgtgacg
    22921 gcgatcccct gctttatcac cagcattctg gcgggttgta tatcaggctg gataaacctg
    22981 aaaatcccca aagcacagcg ctggcgcgtc ggtattctcg gcggcatgtt gtgtgagacg
    23041 ttgaccatga ttctggtaat tgtctgggca ccaactaccg cgttggggat cgatatcgtc
    23101 tctaaaatcg gcattccaat gatcctcggt agcgtctgta tcggctttat tgtgcttctg
    23161 gtgcaaagcg ttgagggcga aaaagaggcc agcgccgcgc ggcaggccaa gctggcgctg
    23221 gatatcgcca acaaaacgct accgctgttt cgccatgtca atagcgagtc attacgcaag
    23281 gtctgcgaaa ttatccgcga tgacattcac gccgatgcgg tggcgattac taataccgat
    23341 catgtgctgg cctatgttgg cgtgggtgaa cataactatc agaatggcga tgacttcatt
    23401 agcccgacta cccgtcaggc gatgaattac ggaaaaatca tcattaaaaa caatgatgaa
    23461 gcccaccgca caccagagat tcattccatg ctggtgatcc cattgtggga gaaaggggtc
    23521 gtgaccggaa cgctgaaaat ttactactgc cacgcgcatc agatcacctc gtcattacag
    23581 gaaatggcgg tcggtctgtc gcaaatcatc tccacgcaac tggaggtttc acgcgccgag
    23641 cagctacgtg aaatggcaaa taaggcagag cttcgcgccc tgcaaagcaa aattaatccc
    23701 cattttctgt ttaacgctct gaacgctatt tcatcgtcaa tccgtctgaa tccggatacc
    23761 gctcgccagt tgatctttaa tctgtcgcgt tatctgcgct ataacattga attaaaagac
    23821 gatgagcaaa tcgatatcaa aaaagagctg tatcaaatta aagactatat tgccattgag
    23881 caggcccgct ttggcgacaa gctgacggtt atctatgata ttgatgaaga ggtgaattgc
    23941 tgcattccca gcctgctgat ccagccgttg gtggagaacg ccattgtcca cggtattcag
    24001 ccttgcaaag gtaaaggcgt tgtcaccatc agcgttgcag agtgcggaaa tcgggtacgc
    24061 attgcggtgc gagataccgg gcacggcatc gatccaaagg tgattgagcg ggtcgaagcg
    24121 aatgaaatgc cgggcaataa aattggcctg ctgaatgtcc atcatcgcgt gaagttattg
    24181 tatggcgagg ggctgcatat ccgccgcctg gagccgggga cggaaattgc gttttacatt
    24241 cctaaccaac gcaccccagt cgcctcacag gctacgttat tgctttgagc cggagtgata
    24301 ttgtgaaagt catcattgtt gaagacgaat tcctggcaca acaggaactg agctggctaa
    24361 ttaaagagca cagccagatg gagattgtcg gcacctttga cgacggtctg gacgtgttga
    24421 agtttttgca gcataaccgc gtcgacgcca tttttctgga tatcaatatt ccgtcgctgg
    24481 atggcgtgtt gctggcgcaa aacatcagcc agttcgccca taaaccgttt attgtgttca
    24541 tcaccgcgtg gaaagaacat gcggtagaag cgtttgaact ggaggcgttt gactacattc
    24601 tcaaaccgta tcaggagtca cgtattaccg ggatgctgca aaaactggaa gcggcctggc
    24661 aacaacagca gaccagcagt acgcctgccg cgacggtaac gcgtgagaat gacaccatta
    24721 atctggtgaa agatgagcga ataatcgtca cgccaattaa cgatatctat tacgccgaag
    24781 cgcacgagaa aatgaccttt gtctatacgc ggcgtgaatc ctacgtaatg ccgatgaaca
    24841 ttaccgaatt ttgcagcaaa ctgccgccgt cgcatttttt ccgctgccat cgctcatttt
    24901 gtgtcaatct gaacaaaata cgcgaaatcg aaccgtggtt taataacacc tacattctgc
    24961 gactgaaaga tctggatttt gaagtgccgg tcagccgcag caaagtgaaa gaatttcgcc
    25021 agttaatgca tctttaatcg ggaactttca tgaaagcacc aggcttgccc gccgatcaac
    25081 aatttttcgc cgatctgttc agcggcctag tgcttaaccc gcaactactg gggcgcgtct
    25141 ggtttgccag ccagcctgcc tcgttaccgg tgggcagttt atgtattgat tttccccgtc
    25201 tggatatcgt gctgcgcggc gaatacggca acctgctgga agcaaagcag cagcgaatgg
    25261 tggaaggaga aatgctgttt attccggcac gcgcggctaa tttaccgatc aacaacaaac
    25321 cggtgatgct gttaagcctg gtgttcgcac cgacctggct tgggttatcg ttttacgata
    25381 gccgcaccac gtcgttgttg catcctgctc gccagatcca gcttcccagc ctgcaacgcg
    25441 gtgaaggtga agcgatgctt accgccctca cccatcttag ccgttcgccg ctggagcaga
    25501 atatcattca gccactggtg ttaagtttgc tgcatctttg ccgcaacgtg gtgaatatgc
    25561 cgccgggtaa ttcgcaaccg cgcggcgatt ttctctatca cagcatttgt aactgggttc
    25621 aggataatta tgcccagccg ctcacccgcg agagcgtggc gcagtttttt aatatcacgc
    25681 ccaatcatct gtcaaaactg tttgctcagc atggaacgat gcgttttatc gagtatgtgc
    25741 gttgggtgcg aatggcgaag gcgaggatga ttttgcagaa atatcatctg tcaattcatg
    25801 aagtggcaca gcgttgcggt tttccggata gcgactattt ttgtcgcgtt ttccggcgtc
    25861 agtttggtct gacgccggga gagtacagcg cccgttttca gggctaacgt cagaaggtta
    25921 attctgtttc cagcagcgtc aggatacttt gtgcatcttg cgcggcaaac aacgactggc
    25981 ggaagttttt attcaccagt ttacgcgcca gctgcgagaa gactttcaca tgattcatcc
    26041 cttcgttagc acccagcgtc agcatgatca ccagttcgac ttcgcccatt tctgactgcc
    26101 agtcaaccgg tttcgccagc cgggcaatgc tgatactgga atgacggatc cactgagatt
    26161 tggtgtgcgg gatcgctacg ccaaaaccaa cgccggtggt aacaatctct tcccgctgcc
    26221 agacatcttc ttccagctca aacggatgtt cagtgcgccc gttaacgccg aggttgccgc
    26281 acaggaactg gatcgcctgc tctttattgc taaaatcctg atcaacaaag atattctcca
    26341 gcgccagcag tgggcgaacg tcttcttccg gcgtaaaggc ggtgagtaac gcttcaattt
    26401 cctgcgcact gcggcattca catgcctgac gcgccagttc ccgacacgcc tcgctatcca
    26461 gttgacgaag ctggcttttc accgccggaa tacgcgggct actcatactc agctcatcca
    26521 ggcccagccc aagcagtagc ggcagataac ggctttcacc gcccagttca ccgcaaatgc
    26581 ctacccattt gccccgctga tgcgcagtgg taactatttg ctgcaacatg cgcaggaacg
    26641 atggcgtaat cgggttatat agcggcgata cgcgcgggtt attacgatcg accgcataca
    26701 gatactgggt catatcgttg gagccgatac tgaagaaatc gacctcatcg cagaagtggt
    26761 cgatgatgta gcacaccgac ggcacttcca ccatgatccc aagcgtaatc gtctctgcat
    26821 gacgcaggcc atcgcgctta agctcaacga tcgctttttg gatctcgcct ttcacccata
    26881 agatctgatc gaggctgtga accatcggga tcatcaactg ggcgttgccg aaactggcgg
    26941 cgcgcaaaat ggcccgcagt tgagtgcgga acaggccagc aaattccggg taaatacgta
    27001 ccgcgcgata gccgaggaat gggttctctt cctggggaac attcagataa gaaatgcttt
    27061 tatcgccgcc gatgtccatc gtgcggaaga taatcggctt gtcgcccgcc gccagtagca
    27121 cctgctggta ggcttcaaat tgctcctgct cgtccggcgc gctgtcgcga tccatataga
    27181 gcatttcggt acggaacagc ccgacacctt ccgcgccgtt ggcaaacgcg cctggcgctt
    27241 ccagagcggt gccgatattc gccgcaatat caatacgctt gttatcacgg gaataggcca
    27301 gctgcgcggc agcctgcgcc tgttgttttt ggcgtttatc cgccagcgtc tgcgcgacct
    27361 gataataacc gctcaccgcg tcattcgggt taatcgccag cacgccgcac tgggcgtcaa
    27421 gcactgcagg ttgcccggca taacgggcaa tcgcatccag cggcaagcca ctcagtaccg
    27481 ggatcgccga ggcacgggcc agaatcagtg tatgcgaggt gcgcccggtt ttctccagaa
    27541 tcatacccgc aagatttttc aaatcaaggc tcaaaaactg gcttggggtt aaatcttcag
    27601 ccaccagaat ggtcggtttt tccagcacca gattgttgcg cggcttcagt tccggccagg
    27661 tgatatgcag caactgttcg ctgatatcgc gaatgtcgct gacccgctcg cgcagataat
    27721 cgctggcaga agcagatagt ttggcgcaaa cctgctccat attgctgatg atcgccgccc
    27781 ccagtccctg atgctgttct gtcatcaggc gacggatatt gcctgcaaat tcatcatcct
    27841 gaatcagcga caaatgagcg ctgaggatag ttttgctttc gccgtcacgc tcacgcaatt
    27901 gctgattcag ttgctcggca agcgttgcca ggctgtgctc cagccgggtg gaatcttgcg
    27961 cactggcggg gattgcccga taactgtcga ggctgtcgct ttgtaacagg gtcagcgtac
    28021 ccacgccgac gccgcttgcc agcacattgc cgtacagtaa atccgggtta aggcggctta
    28081 atgaacgcgg cagcggatgc gccgtcagtt ctgcctgcgt cggctgaacg ctgtcgctgt
    28141 cgataaagcg caccaggatg tactcttcca gcacgcgccg cgcctgctct tcatcgctgc
    28201 cgctaatgtt caggctgcaa ctgtcattaa ataaggtgcc agttccaatc aacgccagcg
    28261 agcttttagc atcggccttt gcgttctggc gatggttgat aaaggtgatt tcgctttgcc
    28321 actggctgca ctgttcttta agttcccgcg ccggacgagc atgtagaccg ttaggcatag
    28381 gcaaaggaca gagaaattga atcgttaaca taaccgctcc gggttaatca ggagatcatc
    28441 tgaaatccgt cagttgaaca accgtctcac gcgtaagacg ttgaataagg gcagataaaa
    28501 gttgctccat ctgcaaaatg tcgcggcaat cggcaatcga tgccgcgcaa tgtccgtggc
    28561 gggtggcagg ccccattacc aatgtgggta cgccagtacc ggttaagtgc accgctccac
    28621 cgtccgtgcc gccgttactg aacatatccg cctgcaacgg aacgccaatt tctgccgcca
    28681 cggtttcaat ccacgcggta agttttggcg gcgcaatcag cgatttatcg cttaacacca
    28741 gcatcggccc gttaccaatc tgacgatggt tagccgcgcc ataatcaaag tttttcgccc
    28801 agcaggcggt atcaagcaca atggcgacgt ccggcgacac cgcgcgggta gcagtttgcc
    28861 cgccgcgtaa tcccacctct tcgctggaac ttgccaccag ccacacttcc gcaggcagtt
    28921 cggcgtcgtg cagctcacgt agtaacgtca ccagcagata gcaaccgagg cgatcatcaa
    28981 aggctttgcc catcacccgc tggtgaggga ggacctgaaa agtggtatcg aaggtgacgc
    29041 gatcgcctgg acgaattccc gcctgcatca cttcgtcata ggtgcgcgcg ccaatgtcca
    29101 cgcgcatggc gctaacgtca ttcccctgcc ggtcgccgtc aagcaggcct agaattttgc
    29161 actcttcacg ggtggtgatg cgcaccggct gcagctggcg ggcagccatg cgcacgttgc
    29221 caaccggcag cacgtcaata gcgccttcgc gggagatgct gcgcaccata aatcccactt
    29281 cgtccatatg ggcgcagatc atcaccttcg gacctgtcga ttcattgagg cggatcagca
    29341 ccgatcccag accatcaaat cgcacttctt tttgcaggcg agccgcttct tccagcagga
    29401 tctgccgcac ttcctgttcc gaggaggcga tcgcatctgc ctcgctcaac gcttttaata
    29461 gcgataaatc cattatgcct ctcccgtgag caacactgtt ttcggcatgg cgtagagcac
    29521 ttctgcgcct tgcggggtaa ccagcacaac atcttcgatg cgcacgcccc cttgccctag
    29581 caaataaatc cccggctcca cggtcagcaa catgcctggc tgtagcgtcg tggtgtcccg
    29641 cggtgaaaaa cgcggatctt catggacttc aatgccgata gcgtgaccag tgttatgacc
    29701 gaaatagtcg ccaaatccag actcggtaat gacccggcgc gcggcgtcgt caatctgctg
    29761 gcagcgcacg ccggggcgaa ttgcagagat tgccgcgagc tgtgcctgca ggacaatctg
    29821 atagacgtca aacagcgggt gagattcggc gctcacccct tcgccattca ccagcaaggt
    29881 gcgcgtcata tcagcagtag ccctgataca gcgcaccaaa atcgagagtg acaaactcgc
    29941 ccgctgcaac aatcttgtcg ctggctttgc cgtgcggcag cgccccgcgc cagccgctgg
    30001 cgacaatggt gtcaaaagag gctttttctg caccctgctg gcgcataaac cactccagtt
    30061 cagcggctat ctcgcgctcg ctcatccccg cctgaataaa gcggcgaata tgctccgctc
    30121 cgcgatcggc aatcccacag gcgaggcgga ttatctccac ctcctctggc gttttgattt
    30181 gccgcagcac atccggcgtg gcgctaacca gtttcgcatt gagttcagac ttccagcggt
    30241 gcgcggtttc ccaactcacc tgctggcctt caaaaccgag cgtttgcaac tgctcatcgg
    30301 caatgatttg attgacgata gtggtaagcg tgttcgtcgc gtcaagcaaa tgcagctggt
    30361 agccttgcgc acgggcttcc acctcaacga aatagcgcga atctaccaga atgtgcgcac
    30421 tttcacggct aataaccaca tagcctgatc cggtggagat ccccagatgc ggctgtttgt
    30481 tctgccgtga ggagagaagc accgcatcca gttgttgcac cttaagccag ttgcgcagcg
    30541 aagcgagtaa tgtcatctgg attttccttc ttacaggcta tcgattaaca atttgccttt
    30601 gcggaacatc atcagacgca ggaacaccac catcagcgca gtaatgattg cccccagcgc
    30661 gatccccgcc atataaacgc ccaggttagt gaccagcggc catgcccaga tagcggattc
    30721 cgggaaccat tgcactgcgc ccagccagac agctgcggtt gagccgacaa tcgcgccgac
    30781 catatatgac ggaatggcgg tgatggggct ttccagcgca aacggaatcg caccctcact
    30841 aatccccata aaggccagga acatcgcggt cttaccctgc ggataaagtt gagcgttgaa
    30901 caggcgtttg ccggttaaac gacggtcaat aatggtcgcc agacccagac caatcggcgg
    30961 aataacgata gcgatagaac gagcggtgac cggtaatacg tggtcagtgg taaagctgaa
    31021 ggcaacgaaa cctgcggctt tgttgatcgg gccaccaagg tcgattgctg tcgccgcggc
    31081 gatccccatt gcgtacatca gcgcgccatt ctcacctgcg gcagtcagca cagtacggat
    31141 accgccgttg atccagccac caaagggggt gatgacgtag tacatcgcca gcatgacaaa
    31201 aatggcggaa agaatcggta gcaaaaatgt ggttttgaac gctaacagga aatccggtaa
    31261 ctggattttc tggttcatcc atttcacaag gtaaccggca acgatagaaa taatcagcgc
    31321 accaatgaag gtggacggca ccggcgaaga ggtcgcccat tgcatagtgc tgggatcgaa
    31381 gttcagcagt tgcgtcggct gggtagacat caacccgccg ataaaaccag ccgggaacgc
    31441 cagtttgcca ccgatagagt tcgccacaaa tgcagcaaac atcggaatgg caaaaccaaa
    31501 caacacgccg ccgaaggact gcgacagcca ggcaaattta agtaaggaga ggtcgaaacc
    31561 ggagaatttc ccgctattaa gagcatccat gatgccgatc tcagcaggaa ttttaagcca
    31621 gctataagca atcagctggc tgaaagcgag gatcacaccg cccataatta aggtcggcac
    31681 catgcgtgaa atccctgaca tcacatgctg cgggagttca ccccagaagc tgcttttaga
    31741 ggcctgcgga ttcttaactg ccgctgccgc accggatgcg ccaggcacaa cggttgcact
    31801 gcgtttttta atggccataa caattatccc tgtcgaatat tactgttgtt cagaggcaat
    31861 catctcttcg atttctttga tgatgcccgc agcgttttta attgcgtcct gcaaagtgat
    31921 ttcataaacg tcgcgtgatt cgaaacgttc gttatcttcc ggggtaactg ccacggaatg
    31981 gatgatgatg gtcgcttcgg cgatatcctg cgccgtcagg cgattctgta tgccgtccgc
    32041 gccctgggtt tcaattttca cttcataacc ggcttctacc gccgcttctt ccagcgcctg
    32101 agcggccata aaagtgtgag ccaggcccat tgggcaggca cataaggcaa tcagtttctt
    32161 actcatcgtt aattcctcgt tcagtgatag ttgacgagtg aaattatgaa cgtgcaaaac
    32221 aaatcgccgt taccagacaa ataatgcatt tactggagaa ttaatcccgc tgtacggaag
    32281 tgtgacgcag gtcggccttg tgaggggaca caaagaaaaa ttgaattaac tgtatgattt
    32341 aaaagattat cgggagagtt acctcccgat ataacaggaa ggatttacag aatgtgacct
    32401 aaggtctggc gtaaatgtgc accggaaccg agaagacccg gatagtcatg gacgatgaga
    32461 tacaccggaa tatcatggac atattcttta aagcgccctt tatcttcaaa tgcggcacgg
    32521 aaaccggagg ctttgaagaa ctcaaggaag cgcggcacga taccgcccgc aataaacacg
    32581 ccgccaaatg tcccgagatt gagcgccaga ttgccgccaa aacggcccat aatgacgcaa
    32641 aacagcgaca atgcgcggcg gcaatcggtg cagctgtcag ccagcgcgcg ttcggtaata
    32701 tcttttggct tgagattttc tggcaggcgg ttgtcagctt tcacaattgc gcgatacaaa
    32761 ttcaccagcc cagggccaga aagcacgcgc tccgccgaaa catgaccaat ttccgcacgc
    32821 aatatttcga ggataatggc ctcttcttca ctattcggcg caaaatcaac gtgaccgcct
    32881 tcgcccggca agcttaccca acgcttatcg acatggacca gatgcgcaac cccaagcccc
    32941 gttccggcac cgtaaaccgc aataggctta ccttcgaccg gttctgcgcc accaaactga
    33001 atcagatgct cttttttcag catcgggatc gccatcgata cagcggtaaa atcgttaata
    33061 atttccagat ggctaaaacc gagattcttt ttcatttcgg caattgagaa cgcccaggta
    33121 tggttggtca ttgccaccca gtcaccggta attgggcaag cgatggcaat acagccgtct
    33181 ttcacctcga ccttatgttc ttcaagataa acgcgaatga ccgcttcgag gctggggtaa
    33241 tcaagccctg aataggtctt agcctgcgag atttcaccac tggcaatatc acacagagca
    33301 agacgtgcgt tggtgccgcc cacatcaccg actaatgcat actttgtcat tcttcaactg
    33361 ctccgctaaa gtcaaaataa ttctttctca cactgtaaat acctgggggc ataacaacaa
    33421 cgccggaaag gcaggctccc tgtaaatatc gatctgggtc acacaattac tttatcgttt
    33481 cagcaccaat tgcagcgatg cctttttgca agctgggcaa actaagtatc tgaccccgca
    33541 taaggaatag aacatgctcc atccgcgagc cagaaccatg ttgttattat cgctccccgc
    33601 cgtggcaatt gggattgcgt ccagtcttat tctgattgtg gtgatgaaaa tcgcctcggt
    33661 attacagaat ttgctctggc aacgactgcc gggaactctg gggatagccc aggattcccc
    33721 cctctggatc atcggtgtat taacgctaac gggtattgcg gtggggttgg ttatccgttt
    33781 cagccagggt catgccggac cagaccccgc ctgtgaaccg ctgatcggcg caccggttcc
    33841 gccctctgcg ctacctggac ttatcgtagc attaattctc ggtcttgctg gcggcgtcag
    33901 cctggggccg gaacatccga tcatgaccgt caatatcgcc cttgccgtcg ccattggcgc
    33961 tcgtctgtta ccgcgcgtca accgaatgga gtggactatt ttagcctctg ccggaaccat
    34021 cggcgcgctg tttggtacac ctgttgcggc ggcgttgata ttttcgcaaa ccttaaatgg
    34081 cagtagtgaa gttccgctat gggatcgtct ttttgcgccg ttaatggcgg cagcagctgg
    34141 tgcacttact accggattat ttttccatcc ccatttttca ctgcccattg cgcattacgg
    34201 acagatggaa atgaccgata ttctcagcgg tgcaattgtc gcggcgattg ccatcgcagc
    34261 agggatggtc gccgtatggt gcttaccacg gttgcacgcg ataatgaatc aaatgaaaaa
    34321 tccggtgctc gtgctgggta ttggcggatt tattctcgga attctggggg ttattggtgg
    34381 accagtttcg ctgtttaaag ggctggatga gatgcagcag atggtggcaa atcaggcttt
    34441 cagcaccagc gattactttt tgctggcggt aattaaactt gccgccctgg tcgttgctgc
    34501 cgccagtggc tttcgcggtg ggcgaatctt cccggcagtg tttgtcggcg tggcattagg
    34561 gttgatgctg catgagcacg ttcccgccgt tccagcggca ataaccgttt cctgcgctat
    34621 tctcggcatc gtgctggtgg taacacgcga tggctggtta agtcttttta tggcggcagt
    34681 cgttgtaccc aataccacat tgctaccgct gctctgtatc gtcatgcttc cggcatggct
    34741 gttattagca ggtaagccga tgatgatggt caatcgtccg aagcaacagc caccccacga
    34801 taatgtttag caaaaaatgc tctcctttat gatttaagag ttatggcgat gatacggggc
    34861 tttacctccc cgtaatattg cgttaacagg ccgctgacgg cttcgatcgt gaaggagaat
    34921 aacaatgttc aggtcactgt ttctggcggc cgccctgatg gcatttaccc cgcttgcagc
    34981 aaatgcaggt gaaatcaccc tactgccatc gatcaaatta caaattggcg atcgcgatca
    35041 ttacggtaat tactgggacg gtggtcactg gcgagatcgt gattactggc atcgcaatta
    35101 tgaatggcgc aaaaaccgct ggtggcgtca tgataatggc tatcatcgtg gctgggataa
    35161 gcgtaaagca tatgagcgtg gctatcgtga aggctggcgc gatcgtgacg atcatcgcgg
    35221 aaaaggccgc ggacatgggc accgccatta aacgcgtcct ccaatggagc acaatgcctg
    35281 atgcgacgct cattcaacta caatcccagc gccgtcccca ccaacaacca gatattcagc
    35341 gccacgacca gcactacaat cacccagcct gtctgtttta cgcgtttgct gttcaccaga
    35401 tcgcccatca acttgctgtc actggtgaaa atcagcagtg gaaccagcgc cagggcgata
    35461 ccaaaactta acagcacctg actcataacc agaatccgtg tcggatctaa tcccatcaga
    35521 atgacaataa atgacggcaa catggtgact gtacgacgca cccacagcgg gatatggaag
    35581 cgaatgaatc cctgcatcac cacctgcccc gccagtgtcc ccaccaccgt tgaggacagt
    35641 ccggcagcaa ccagacttaa cccaaagacc gttgccgcag catggcttaa cagcggttgc
    35701 agcgtcagat aagcctcatc aagatcggca acaccagtat gaccagaaaa gtggaacgcc
    35761 gccgcagctg tagccatcat cgccagattg acaaaaccgg caatcgtcat ggcgatagcc
    35821 acatcccatt tggtggcgga ataacgttgt tgacgcgaac cgccatgtaa atgctgagtg
    35881 agcgaagagt gcaaataaat cacatgcggc ataatcgtcg cccctaacac gcctgctgcc
    35941 aggaagaccg cctccgaagt aggtaaactc gggatcacca ttcctttacc cagctgcgcc
    36001 aggttaggct gggagaaaat caactcgaca atgtaagccg cggcaacaaa caacagtaac
    36061 ccgccaatca ctttctccag cggtttttgc ccgcgacgtt gcagcattaa aatcaggaaa
    36121 gtcgcgatcc ccgtcagcac cgcgccctgc aacaacgaaa caccaagaat gagtttaaaa
    36181 ccgatcgccg caccaataaa ttccgccagg tcggttgcca tcgcaataat ttctgcctga
    36241 acccaataga accacactac gggacgcgga tagtgatcac gaatctgctc cgccagattt
    36301 ttaccggtgg caatccctag tttggcagag aggatctgaa ttagcatcgc catcaggttg
    36361 gcccaaacga caacccacag tagctgatag ccgaagctag cacccgcctg aatattggtc
    36421 gcaaagttac cgggatcgat ataaccaatc gccgcaatga acgcaggtcc cattaatgcg
    36481 agcctcatct tgcgcgccgc ccgtccgctg ctactctcaa cgcgatagtt cgtcatcttg
    36541 tgcctctaaa acatagcctt tgctatgttt catgctatgc taaacgagaa tgattatcaa
    36601 attcatttaa atggattgtg gtgatttctc tgatagacca agattatgat tacgaaaaga
    36661 ttgatgactt gaatgttatg gttatgttta atgttagcac atttacataa ctttcagctt
    36721 ccatacacaa catagcagaa atgtatgaca gatcactatt tttgaagcct gtcacaggac
    36781 gtcattatag tgtgtgtcag atctcgtttt ccttaaccat gttacataga atgtgcacgg
    36841 aaatttaacc tgcctcatat ttggagcaaa tatggaccgc gtccttcatt ttgtactggc
    36901 acttgccgtt gttgcgattc tcgcactgct ggtaagcagc gaccgcaaaa aaattcgtat
    36961 ccgttatgtt attcaactgc ttgttatcga agtgttactg gcgtggttct tcctgaactc
    37021 cgacgttggt ttaggcttcg tgaaaggctt ctccgaaatg ttcgaaaaac tactcggatt
    37081 tgccaacgaa gggactaact tcgtctttgg tagcatgaat gatcaaggcc tggcattctt
    37141 cttcctgaaa gtgctgtgcc caatcgtctt tatctctgca ctgatcggta ttctccagca
    37201 cattcgcgtg ttgccggtga ttatccgcgc aattggtttc ctgctctcca aagtcaacgg
    37261 tatgggcaaa ctggaatcct ttaacgccgt cagctccctg attctgggtc agtcagaaaa
    37321 ctttattgcc tataaagata tcctcggcaa aatctcccgt aatcgtatgt acaccatggc
    37381 tgccacggca atgtccaccg tgtcgatgtc catcgttggt gcatacatga ccatgctgga
    37441 accgaaatac gtcgttgctg cgctggtact gaacatgttc agcaccttta tcgtgctgtc
    37501 gctgatcaat ccttaccgtg ttgatgccag tgaagaaaac atccagatgt ccaacctgca
    37561 cgaaggtcag agcttcttcg aaatgctggg tgaatacatt ctggcaggtt tcaaagttgc
    37621 cattatcgtt gccgcgatgc tgatcggctt tatcgccctg atcgccgcgc tgaacgcact
    37681 gtttgccacc gttactggct ggtttggcta cagcatctcc ttccagggca tcctgggcta
    37741 catcttctat ccgattgcat gggtgatggg tgttccttcc agtgaagcac tgcaagtggg
    37801 cagtatcatg gcgaccaaac tggtttccaa cgagttcgtt gcgatgatgg atctgcagaa
    37861 aattgcttcc acgctctctc cgcgtgctga aggcatcatc tctgtgttcc tggtttcctt
    37921 cgctaacttc tcttcaatcg ggattatcgc aggtgcagtt aaaggcctga atgaagagca
    37981 aggtaacgtg gtttctcgct tcggtctgaa actggtttac ggctctaccc tggtgagtgt
    38041 gctgtctgcg tcaatcgcag cactggtgct gtaaaaccat acataaaaaa gccggggatc
    38101 attccccggc ttttttatgc ctgttaatca gctaatggct gcgggcgacc aatcaaatac
    38161 ccttgctgat attgcacccc gagcttatgc aatagcgcct gctgctgttg cgtctcgaca
    38221 aactccgcga ccacactcaa tgacttcgct ttcgccagat cggtaattga tctcacaatc
    38281 atcgcatcca gcgtattcgt gacaatatct ttcacaaaga cgccatcaat tttgatgata
    38341 tcagcctgca aacgctttaa ccgttcgtag ttggcatatc cggcgccaaa gtcatcaatc
    38401 gcaatccgga aaccaaactt atgcagctgc tcgatgttgt acatgctgct ttctgcgtta
    38461 gaaaacgcct gctcctcggt gatctcaaga atgaccgcct gcggggagat gtgataacgc
    38521 ttaaacagac gaataatccg cccggcaata ttcttttgca gcagcgtgag cggcattaaa
    38581 ttgactgaaa agcgcggtcc ttttttgtcg caagggtgtg ttgccagcca cttcaacaag
    38641 gattccagca cttgcaaatc aaaacgcgcg ctaaggttaa actgagcaat aaggggcaga
    38701 aacttatccg gggtcataat gccgccgtca tatttcagtc gcgcgaggat ctcatcataa
    38761 ccttcaccct ctttgttgcg aattggctgg gcgtagagca gcaaatcacc ctgatctaac
    38821 gccgtgcgaa tggtattcag caatagcacc tgtttagtgg tctgcccgga aaccatctcc
    38881 tctctgctat ccagcgccag cacatgatga tgtgcgcagg attgctccgc cagccagctt
    38941 aactgcccca acaagggttg cagggtttcc tgatttccat caaaacgccc ccaggcagca
    39001 ccatagccca tatccagccc ggtattgttc cagtgaattt gccgactatt caggatgtta
    39061 accatatgct ggagtcgccc ttccgtttcc ggcccgctca gcactaacag cagttcacta
    39121 cccggcaatt gatacaactt ttcgttttcc tgcatcaacg gcagcagcgt acggcaaatt
    39181 gagcggatac agtgaacgcg catcattaag ccgtaatgac gactcataaa ctcaagatta
    39241 tcaatgcgca ggcagcaaaa actcttgccc gcctcttgct ccggcgcttg ttccaacgca
    39301 cgaaagttgg gtaggagcgt taacggatct gttagcgcct gcaaatgcca gcggcgatta
    39361 agccattcac tgcgatgata aatgcgcacc atatagagca ggcaaacgct aaaggaaatc
    39421 agcaccgcga gaataaatgc cagcgaatat tcggtttcca ccccttgcaa aaagttctgg
    39481 ttgtaattca gaaggcaaag cgttgaaaca gcctaggtga gatttaaaaa cggatagcga
    39541 agattaccga ccccgagggt aaagatgatg aagaaaacgg gtaccaggta accggcaata
    39601 aagtcgtttt cataaggtgt gcacaacaga agtaggagca cgcttaacgc tgccagccag
    39661 cttaaggtaa atgcgcgttt ctctttgccc aacgacggag cgatatccct gcgccacaat
    39721 atctgcgcaa agtggggact tacaatcatg cgagtgagat agtagaaaag catgttgtaa
    39781 atcagcactg cggtgaaaag gcttagtaaa tcaacgaccg tgaaaatggc atccgcatcg
    39841 ccgaaaaagg tggatatctt tagcgggaaa tcaaagaagc tccccacaag atacatgctg
    39901 catttgatgc cgatcggcgt caccaaacca agccagaaca gacgttgcca gacatatcgg
    39961 ctggtcaatc cataacgcca acgcgtcccc agctgccagc gtaatattgc gcaggcacac
    40021 aacacgacaa acgtctggct gccgagcatg accgcagtct gtaaaaaaga gagatgaaag
    40081 ttccacagat tggtgcaaaa catcccgcat aaaacgggaa cgacaccgcg ccagccaaag
    40141 ataaacaaga ttgacagcat tacgcacagt ggcatccacg ccagaaagat ataactggaa
    40201 tggataatgg caagtggcga aataaaacgg gatagctgaa taagtaccac ggtgagcgta
    40261 aacgctagtg tgaatatctt gatatttttt atcaggttat gctccacaaa catgagtaca
    40321 ctactctcac tgatcggggt tttcaggcac acaaataata agtttattcg catcgcgaag
    40381 caaacagggg ccagcctgtt gccgattttt catccaatga tgctttcacc aggcggcgct
    40441 aacccggacg taaactgtat tacaaaagcg gcaaaaagca gagacaaaaa acccccgctt
    40501 tgcagcgagg gttggaaatt tggtggagct aagcgggatc gaaccgctga cctcttgcat
    40561 gccatgcaag cgctctccca gctgagctat agccccacga tgcgtttacg taccaaattt
    40621 gctgggtgca aaatttggtg gagctaagcg ggatcgaacc gctgacctct tgcatgccat
    40681 gcaagcgctc tcccagctga gctatagccc cgtcacgtaa agcttgtcga gttgacgggc
    40741 ggcatcatat gaattccgcc cgaatgtgtc aacggcaaat tgcaacgtgt agtttcaatc
    40801 gctgaaaaat caggcaaatg aacaattttg gaaaaccgct cgcattcagt agttattcat
    40861 gtcacggttt cctgtaaagt ggtgttataa aatgaactac taatagaccc acatacattc
    40921 agggaattgt tatgtccaag gagaggatga caccagatga acttgccaga ctgaccggct
    40981 atagccgcca gaccattaat aaatgggtac gcaaggaagg ctggacaacg tcaccaaaac
    41041 ctggcgtcca gggtggcaaa gcccgactgg ttcacgtcaa tgaacaggtt cgtgaatata
    41101 ttcgcaatgc tgaacgtcca gaaggtcagg gagaagcgcc tgccctttct ggtgatgcgc
    41161 cgcttgaagt attgctggtg acactggcga aagagatgac gccagttgaa caaaaacagt
    41221 ttacatcctt gcttctgcgg gaagggatta tcggattgtt acaacgctta gggattcgcg
    41281 atagcaaata atatgaaaag attacgcaat aaaatgacca ccgaagaact ggctgaatgc
    41341 ctcggtgtgg ctaaacaaac tgttaaccgt tggatcagag aaaaaggctg gaaaacggaa
    41401 aaatttcccg gcgtgaaagg cggtcgtgcc agacttattc tggtcgatac gcaagtttgc
    41461 gagtttattc agaacacgcc agccttccat aacacaccaa tgttgatgga agctgaagag
    41521 agaattgctg aatacgctcc tggagcacgc gccccggcgt atcgacaaat catcaacgcg
    41581 atagataata tgacagacat tgagcaggaa aaagtcgcgc aatttttgtc acgtgaagga
    41641 attcgcaatt tcctcgcccg tctcgatatt gacgaatcag cataaataaa aaacggcagg
    41701 atattatctc ctgccgttta tctttttaca cgctaattac tgctgatttt cgcgttcagc
    41761 aataaaatcc agcgctttgt tgatacgctc gatactgcgg gtcttaccaa ttgcgtgaac
    41821 ggtaacatcc agtgctggag actgccccgc accggttacg gcgacacgca gcggcatacc
    41881 aactttaccc atacccactt ccagctcatc cgccgtcgcc tgaatagcgt gatgaacgtt
    41941 ttcagcggtc cagtcagtaa tcgcggccag tttgtcacga accacttcca gcggctgacg
    42001 cgctaccgga cgcagatgtt ttttcgccgc atcggcatcg aactcagcaa aatcttcgta
    42061 gaaataacgg cagctctgtg ccatctcttt cagcgtcttg cagcgttcgc ccagcagttt
    42121 caccagatca gccagctgcg ggccgttacg ggtatcgata ttttcctgct caatgtgcca
    42181 ctgtaagtga gtagcaacat actccggcgg cagcgcgtta atgtagtgat ggttcagcca
    42241 cagcagcttg tcggtgttga acgcactggc agatttgctg acggcattca aagtgaagta
    42301 tttgatcatc tcttcacgag tgaagatttc ctgatcgccg tgggaccagc ccagacgcac
    42361 cagatagttc agtagtgctt ctggcaaata accgtcatca cgatactgca ttacgctgac
    42421 tgccccgtga cgtttggaca gttttttacc gtcatcgcca ttgatcatag aaacgtgcgc
    42481 gtaaaccggc accggcgctt tcagggcctt aagaatgttg atctggcgcg gcgtgttgtt
    42541 gatatggtct tcgccacgga taacgtgggt gatttccata tcccagtcat cgacaaccac
    42601 acagaagtta taggttgggg aaccatcggt acggcggata ataagatcgt ccagttcctg
    42661 gttgctgaac tcgatcggac cacggatctg atcgtcaaaa acaacagaac cttcctgcgg
    42721 gttagcaaaa cgtacaacac acggttcatc atcagcatga tgctcatggc tgtggcggca
    42781 gcgaccgtca taacgcggct tctcaccttt cgccatttgc tcttcgcgca gcgcttccag
    42841 gcgctcttta gagcaatagc atttataagc agtgccctct tccagcatct gatcgatcac
    42901 cgcgttgtag cgatcaaaac gtttggtctg gtagtacgga ccttcatccc actccaggct
    42961 cagccagttc atgccatcca taatggcttc gatagcttcc ggcgtggaac gctcaagatc
    43021 ggtgtcttca atacgcagca cgaactcacc gccgtggtta cgtgcaaaaa gccaggagta
    43081 aagagcagta cgcgcgccgc caacgtgcag atagcctgtt gggcttggcg cgaagcgagt
    43141 tttgattttc atgaaatggc cttacgttta gaaagatgcc gacaaccggc aaatcctgga
    43201 aaaattgagt gggcgatatt ctatcactcc cgcctgattc ctcaatgtag ttcgggtttc
    43261 taccttacgc attattgttt tcgttgacaa attgcgcaac acggtctgtt ttgcgatcat
    43321 cttgcttaaa tttacgacga acgaacaatt tctttagaaa atgcgttgac tcattttgaa
    43381 ctctccctat aatgcgactc cacacagcgg gggtgattag ctcagctggg agagcacctc
    43441 ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccaacta ctttatgtag
    43501 tctccgccgt gtagcaagaa attgagaagt gggtgattag ctcagctggg agagcacctc
    43561 ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccacttc gggtcgttag
    43621 ctcagttggt agagcagttg acttttaatc aattggtcgc aggttcgaat cctgcacgac
    43681 ccaccaatgt aaaaaagcgc cctaaaggcg cttttttact atctgcgata ctcaaagatt
    43741 cgaacctgca gcaggtttga gttgagcgca gcgaaacaac ggagccgctc gcggcgacgg
    43801 cccgaagggc gagcgaagcg agtcatcctg cacgacccac caatgcaaaa aagcgcccta
    43861 aaggcgcttt tttgctatct gcgatttgta agattgattt ttcttctcaa gttccttgtt
    43921 cttctaccga tattacctcg tacagtgacg aggactgaac aatggccact atctcttcaa
    43981 cttcaattcc ttccatacaa acacagagta gtaacagggc atcacaaggt agcgatgttg
    44041 cttcacaaat cgcgcggata agtcagcaaa tcatcaaact cactcaacaa attaaagaga
    44101 ttgtagacac tagcggcagt gctgaagaca agcaaaaaca ggccgaattg attcaacagc
    44161 aaatcaccct actggaaaca caactggcgc agttgcagaa acagcaggca gaaaaggcgc
    44221 aggagaaaga gcagcgtttg tctctgaatg tatcccttct aaatcctgtt gaaaaaacga
    44281 ctcatatcga tatctatatc tgatacaacg gatccccttc ccgaccagcc attagcgcat
    44341 tgccctggtc tcgtaccacg tcccataacg cttcagcggc ggtggaaagt gaacggttct
    44401 tcctgcgcac cagcatcaac tggcgttcaa ccaccggcgt aatgcgctta accaccagtg
    44461 ggctaccttc aggcaatggt agcgccagtg caggaagaat actgatgccg atgcctgcgg
    44521 ctaccatcgg gaacagcgtc gccggatgtc caatctcctg cacaatattc gcctgaatac
    44581 cattgcgcgc cagcgctgcg tcaatcagcg gtcggctgcc tgacgcgtaa tcctgcaaca
    44641 ccaatttcgc tccctgtaat gcctgccagg gtacataatc ttccacggcc aaagcgctat
    44701 cgcgatggca aagcagaaag aaaggctcgg aaagaatcgc ttcacattgc agatcgccca
    44761 ctgggcctgg atcgatgacg atgccaaagt cgacatctcc ctggcgaata ctttccatca
    44821 cccactgctg cggacgatca tgcaggacaa actgaatatc tggatagcgg cgatggcttt
    44881 cagcaatgca ttgcggaata agatgcgcgg aaatggtctg gctggcagcg acccgaactt
    44941 ttccgctcag ttgttgcccc atacgcccgg tatcgcgcaa cgtgctgtta agttcatcca
    45001 gcagtcgctc aagacgcaaa gccaactgct gccctgcatc tgtaagcacc acttcacgcg
    45061 tggttctgtc cagcaggcga acaccagtat gattttccag ctccttcaca ctgtgactta
    45121 ctgccgactg gctcaagccg atacgctctc ctgcacgact aaaacttttc tcctgcgcta
    45181 ctgtgacgaa aacctttaat tgttttaaag aataattcat ctgttttctt catgaatgga
    45241 tgcaataaat caattttatt tctcaaactg gaagaagcac aatagaacca tcgatcatct
    45301 ggagtcttta tgaaactttt tcgtatcctc gatcctttca ccttaaccct gatcacggtg
    45361 gtgttgctgg cctctttctt tccggccaga ggcgatttcg tccccttctt tgaaaatctg
    45421 accaccgcag ctattgccct gctgttcttt atgcacggcg cgaagttgtc gcgtgaggcg
    45481 attattgctg gcggtggtca ctggcgactg catttgtggg taatgtgcag caccttcgtg
    45541 ctgtttccga ttctgggtgt actgtttgcc tggtggaaac cggtaaatgt cgacccgatg
    45601 ctctactccg gttttctcta cttgtgcatt ctcccggcta ccgtgcagtc tgcaatcgcc
    45661 ttcacgtcaa tggcgggcgg taacgtcgcg gcggcggttt gttctgcgtc agcatccagc
    45721 ctgctgggga ttttcctttc accattgctg gttggtctgg tgatgaacgt tcacggtgca
    45781 aggggcagcc ttgagcaggt cggtaaaatt atgctgcaac tgctgctgcc gtttgtgttg
    45841 gggcatcttt cccggccgtg gattggtgac tgggtgtcgc gcaataaaaa atggattgcg
    45901 aaaactgacc agacgtccat tctgttggtg gtttatacag cgttcagcga agccgtcgtt
    45961 aatggtatct ggcataaagt tggctgggga tcattgctgt ttatcgtggt ggtcagctgc
    46021 gttcttctgg ctatcgtgat tgtagttaac gtctttatgg cacgccgact gggcttcaat
    46081 aaggcagatg aaattactat cgtcttttgt ggttcgaaaa agagtctggc aaatggcatc
    46141 ccgatggcaa acattctgtt ccccacatcg gtgatcggta tgatggtgct gcccctgatg
    46201 attttccatc agatccaatt gatggtctgt gcggtgctgg cgcgtcgata caaacgccag
    46261 accgaacagt tacaggcgca gcaggaaagc agcgccgata aagcttaaag cggacgcttc
    46321 aggggctgga ccagttgcgt cagcccctcg gttttaatca gcagcgtgat ttgcattagc
    46381 tcaccgagct tccctgccgg gaactcatct ttgcgggcaa accacagcag atactcctcc
    46441 ggcaggtcaa ttaagcgacg ccctttgtat ttgccaaacg gcattatcgt attggctatt
    46501 tcaatcagct gctctttttc catctcagct acccagcaaa cgcagcattt ccgcttcgtc
    46561 gatgacttca atgcccagtt cctgcgcctt cgccagttta gatcctgcag cttcacccgc
    46621 tatcaccaga tcggttttct tcgacacgct gcccgcgact ttcgcgccca gttcgaccag
    46681 tcgagcttta gcgtcatcac gcgacatctg gcttaagctg cccgtaagca ccacggtttt
    46741 accagcaaac gggctgtcaa tctcttccgc gttgataacg atcggcgcag gccagtgaac
    46801 accttccgcc aacagctcgc tgatgacatt gcggttgctt tcttcggcaa agaagttgtg
    46861 aacgtgggat gcaacgacaa tgccaacatc aggcaccttt tgcagctctt caatcgaagc
    46921 ggcttccagc gcttccagcg tgccgaaata tgccgccaga cctgctgcgg tggcctcgcc
    46981 gacttcacgg atgccaagtg catagaggaa gcgagcaaag gtggtttctt tcgctttttc
    47041 cagcgcgtta accacgtttt gtgccaattt tggccccata cgctccagtc cggtcagttt
    47101 gcctgcggtg agtttgaaca gatctgccgg agtatggaca tattcttttt caaccagctg
    47161 atcgatgatt ttgtcgccca ttccgtcaac atccatcgca cggcgggaaa caaagtgttt
    47221 cagcgactct ttacgctgcg caccgcaaat caggccaccg gtacagcggg caaccgcttc
    47281 accttccaca cgctcaacgt cagaaccaca taccggacaa tgcgtcggga atacaacctc
    47341 acgggtatct tccgggcgtt cagaaagcac gacgttaacc acctgcggga tcacgtcgcc
    47401 agcgcggcga atcaccactt tatcgccaat gcgtaaacca agacgttcga tttcatccgc
    47461 attgtgtaag gttgcgttac tcaccagcac gccagcaaca tggacaggtt ccagacgcgc
    47521 aacaggcgta atcgcgccag tacgcccaac ctgaaactcg acgtcacgca caaaggtcat
    47581 cagctcctgc gccgggaatt taaacgctac cgcccagcgc ggggcacgcg cgacaaagcc
    47641 aagctgctcc tgctgttcca gtgagttgac cttaatcacc acgccgtcga tatcaaagcc
    47701 cagcgtcggg cggtcttctt ccactttgtg atagaacgcc agcacttctt ccgccgattc
    47761 acaaagcgtt acccgatcgc tgaccggcaa cccccacttt ttaaattgca gtaaacggcc
    47821 aagatgagta tccggcagct cgccaccttc cagaacacca acgccatagc agaaaaaagt
    47881 gagcggccgc ttcgctgtaa tacgcggatc aagctgacgc agtgaaccag ctgccgcatt
    47941 acgtgggtta gcaaaaactt tcccacccgt gcgtcgcgca tcttcgttaa tcttttcgaa
    48001 ccccgcctgc ggcaggaaca cttcaccacg cacttccaga cgcgccggga tattctctcc
    48061 gtgcagcttc agcggaatgg cgcgaatagt acgcacatta gacgtgatat cttccccggt
    48121 ggagccatcg ccacgggtcg cggcactgac taaaacgcca ttttcataca gaatactgac
    48181 ggcaaggcca tccagcttca gctcacagca ccaggtgact ttctcgttgt ttttcagacg
    48241 gtcctgcaca cgtttgttga aagcaagaaa gctttcttca tcaaaaacgt tatccagtga
    48301 cagcattggt acttcatggc gtatctggct gaaagctgcc agcggcgcag cacctacacg
    48361 ttgggtaggc gaatcaggcg taatcagttc tggatgtttg gtttccagct cgcgcagttc
    48421 gcgcatcagc ctgtcgtatt cagcgtcggg aatttccggc gcatccatca catgataaag
    48481 atattcatga tggcaaagcg tcgttcgcag ttctgtcagt tgttgttcga ttgattccat
    48541 atcgcaccat caatgctaaa aacccccgac aagcgggggt tcgaagagga gttaatttgc
    48601 cttaagtgta tcaggcgttg gcgtctttga cttcgcggat gatgtcctgg tactcgcgca
    48661 atttctgcgg agtcatcata cggcgctgat cgtcaagcac gacaccgccc acttcatcgg
    48721 caatatgctg cgcagattgc agcatcagct tgaagttctg cagctcgtca ccgtaagacg
    48781 gtacctgcat aaagatagtg acacccggag tagtgaaatc cttcatttca ggatcaaagg
    48841 ttcccggttt caccatattc gccaggctga ataacgccgg gccgctgcca tccgggctaa
    48901 gatgacgatg gtaaatattc atatcgccaa aaatgaagcc cgcttgttga atgctgttaa
    48961 gaagcagttc accgtttagc tcgctaccgt gatgcgccgc gacgttcatg ataatcaccg
    49021 cttctttgcg cttcggttta tccataactg gagccggttc cgctacaggc tcaggctgtg
    49081 gtgccgctac gggttctgca ggctggaaag cctgttgtgc cggttgcggt gctgaatgca
    49141 ctggctgcgg cgctggcgcg acctgtggcg aaactggctg ctggaccggt tgacgcggct
    49201 gcgcagacgc ataaggcggt tggtactggt gttgcggcga cggacgagca gcctcatgct
    49261 cctgagcgtt agccggggca tgattcacgc ggtgaacacg aacctcacca acgccctcat
    49321 catcttcgac atcctcgtca taagaatcgt cgtcacgttt tgacttcatt cgttttaatg
    49381 gccgatcgcg gaacatagaa gatcgttctt tacggctggt ccagaaaccg tgtaccagta
    49441 aagcgattat ggcgatcgcg ccaacaatga ttaatatcag acgcaaatcc tgcatcatta
    49501 tattctctgt tgttctaaca ccttgccacc acggcaaaca tttactcact aagagtattt
    49561 gccgattacc tcaagtgcaa gtgcactatt aactttcaca gcacaaagat agatgaaatc
    49621 gtgctttttg ctgttttttc gaacatatcc taactgtcca ttgcgcaatt acccggtaaa
    49681 atacgcagaa ttttcctggg attggtcaaa aggagctcat cctgattatg gtttcatcat
    49741 tcacatctgc cccacgcagc ggtttttact attttgcgca aggctggaag ctcgtctcgc
    49801 aacctgggat tcggcgtttc gttattttac cgctgctggt caatattttg ttgatggggg
    49861 gcgcattctg gtggctcttt acacagctcg atgtctggat cccgactttc atgagttacg
    49921 ttccggactg gctacaatgg ctgagttatc tgttgtggcc tctggctgtc atctctgtgc
    49981 tgttagtgtt tggctatttc ttctccacga ttgctaactg gattgccgct ccgtttaacg
    50041 gtttattggc tgaacaactg gaagcacgat tgactggcgc tacaccgcca gataccggga
    50101 ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg cgaatggcaa aaatttgtct
    50161 ggtatctgcc gcgcgcaatt gtattactaa ttctttactt catccctggt attgggcaaa
    50221 ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat gttagccatc cagtattgcg
    50281 attacccctt cgataaccac aaagtgccgt ttaaagagat gcgcaccgcc ctgcgcacac
    50341 gcaaaatcac caatatgcag tttggtgctt taaccagcct gtttacgatg atcccgctgc
    50401 ttaatctgtt catcatgccc gttgccgttt gtggcgcgac ggcgatgtgg gtcgattgct
    50461 atcgcgataa acacgcgatg tggcggtaac aatctaccgg ttattttgta aaccgtttgt
    50521 gtgaaacagg ggtggcttat gccgcccctt attccatctt gcatgtcatt atttcccttc
    50581 tgtatataga tatgctaaat ccttacttcc gcatattctc tgagcgggta tgctacctgt
    50641 tgtatcccaa tttcatacag ttaaggacag gccatgagta agatttttga agataactcg
    50701 ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca tcggtaacgg acgcattctg
    50761 gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt gccgtatcgg tgccaacatg
    50821 atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg ttgaactggt tgaaccgacc
    50881 agcggtaata ccgggattgc actggcctat gtagctgccg ctcgcggtta caaactcacc
    50941 ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc tgctgaaagc gttaggtgca
    51001 aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg caatccaaaa agcagaagaa
    51061 attgtcgcca gcaatccaga gaaatacctg ctgctgcaac aattcagcaa tccggcaaac
    51121 cctgaaattc acgaaaaaac caccggtccg gagatctggg aagataccga cggtcaggtt
    51181 gatgtattta ttgctggcgt tgggactggc ggtacgctga ctggcgtcag ccgctacatt
    51241 aaaggcacca aaggcaagac cgatcttatc tctgtcgccg ttgagccaac cgattctcca
    51301 gttatcgccc aggtgctggc aggtgaagag attaaacctg gcccgcataa aattcagggt
    51361 attggcgctg gttttatccc ggctaacctc gatctcaagc tggtcgataa agtcattggc
    51421 atcaccaatg aagaagccat ttctaccgcc cgtcgtctaa tggaagaaga aggtattctt
    51481 gcaggtatct cttctggagc agctgttgcc gcagcgttga aactacaaga agatgaaagc
    51541 tttaccaaca agaatattgt ggttattcta ccatcatcgg gtgagcgtta tttaagcacc
    51601 gcattgtttg ccgatctctt cactgagaaa gaattgcaac agtaatgcca gcttgttaaa
    51661 aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc ctgcttcaaa ctttcgcccc
    51721 tcctggcatt gattcagcct gtcggaactg gtatttaacc agactaatta ttttgatgcg
    51781 cgaaattaat cgttacagga aaagccaaag ctgaatcgat tttatgattt ggttcaattc
    51841 ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc agcggtcaac acccgccagc
    51901 aatggactgt attgcgctct tcgtgcgtcg cgtctgttaa aaactggcgc taacaataca
    51961 ggctaaagtc gaaccgccag gctagacttt agttccacaa cactaaacct ataagttggg
    52021 gaaatacaat gttccagcaa gaagttacca ttaccgctcc gaacggtctg cacacccgcc
    52081 ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc tgaaattact gtgacttcca
    52141 acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca gactctgggc ctgactcaag
    52201 gtaccgttgt gactatctcc gcagaaggcg aagacgagca gaaagcggtt gaacatctgg
    52261 ttaaactgat ggcggaactc gagtaatttc ccgggttctt ttaaaaatca gtcacaagta
    52321 aggtagggtt atgatttcag gcattttagc atccccgggt atcgctttcg gtaaagctct
    52381 gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa atttctgccg accaggttgc
    52441 tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca tcagcccagc tggaaacgat
    52501 caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa gccatctttg aagggcatat
    52561 tatgctgctc gaagatgagg agctggagca ggaaatcata gccctgatta aagataaaca
    52621 catgacagct gacgcagctg ctcatgaagt tatcgaaggt caggcttctg ccctggaaga
    52681 gctggatgat gaatacctga aagaacgtgc ggctgacgta cgtgatatcg gtaagcgcct
    52741 gctgcgcaac atcctgggcc tgaagattat cgacctgagc gccattcagg atgaagtcat
    52801 tctggttgcc gctgacctga cgccgtccga aaccgcacag ctgaacctga agaaggtgct
    52861 gggtttcatc accgacgcgg gtggccgtac ttcccacacc tctatcatgg cgcgttctct
    52921 ggaactgcct gctatcgtgg gtaccggtag cgtcacctct caggtgaaaa atgacgacta
    52981 tctgattctg gatgccgtaa ataatcaggt ttacgtcaat ccaaccaacg aagttattga
    53041 taaaatgcgc gctgttcagg agcaagtggc ttctgaaaaa gcagagcttg ctaaactgaa
    53101 agatctgcca gctattacgc tggacggtca ccaggtagaa gtatgcgcta acattggtac
    53161 ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa ggcgttggtc tgtatcgtac
    53221 tgagttcctg ttcatggacc gcgacgcgct gcccactgaa gaagaacagt ttgctgctta
    53281 caaagcagtg gctgaagcgt gtggctcgca ggcggttatc gttcgtacca tggacatcgg
    53341 cggcgacaaa gagctgccat acatgaactt cccgaaagaa gagaacccgt tcctcggctg
    53401 gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg cgcgatcagc tccgcgctat
    53461 cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc ccgatgatca tctctgttga
    53521 agaagtgcgt gcactgcgca aagagatcga aatctacaaa caggaactgc gcgacgaagg
    53581 taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg gaaacaccgg ctgccgcaac
    53641 aattgcacgt catttagcca aagaagttga tttctttagt atcggcacca atgatttaac
    53701 gcagtacact ctggcagttg accgtggtaa tgatatgatt tcacaccttt accagccaat
    53761 gtcaccgtcc gtgctgaact tgatcaagca agttattgat gcttctcatg ctgaaggcaa
    53821 atggactggc atgtgtggtg agcttgctgg cgatgaacgt gctacacttc tgttgctggg
    53881 gatgggtctg gacgaattct ctatgagcgc catttctatc ccgcgcatta agaagattat
    53941 ccgtaacacg aacttcgaag atgcgaaggt gttagcagag caggctcttg ctcaaccgac
    54001 aacggacgag ttaatgacgc tggttaacaa gttcattgaa gaaaaaacaa tctgctaatc
    54061 cacgagatgc ggcccaattt actgcttagg agaagatcat gggtttgttc gataaactga
    54121 aatctctggt ttccgacgac aaaaaggata ccggaactat tgagatcatt gctccgctct
    54181 ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt ttttgcggaa aaaatcgttg
    54241 gtgatggtat tgctatcaaa ccaacgggta acaaaatggt cgcgcctgta gacggtacca
    54301 ttggtaaaat ctttgaaacc aatcacgcgt tctctatcga atctgatagc ggcgttgaac
    54361 tgttcgtcca cttcggtatc gacaccgttg aactgaaagg cgaaggcttc aagcgtattg
    54421 ctgaagaagg tcagcgcgtg aaagttggcg atactgtcat tgaatttgat ctgccgctgc
    54481 tggaagagaa agccaagtct accctgactc cggttgttat ctccaacatg gacgaaatca
    54541 aagaactgat caaactgtcc ggtagcgtaa ccgtgggtga aaccccggtt atccgcatca
    54601 agaagtaatt cttgccgcag tgaaaaatgg cgcccatcgg cgccattttt tatgcttccg
    54661 ccagcggcgg caaaatcaat tcatcgctct catgctgctg ggtgtagcgc atcacttcca
    54721 gtacgcgcaa ccccgcacgg tgcacagcat cgtttaacgc cttccctttc agcaaaccac
    54781 tgataagctg agcacaaaac aggtcgccag tccctttcag gtcggttttt acccgtgaat
    54841 gggaaatgac attcacgctg tcggcactga ccaccaccac ctgcatctcc tgattttctt
    54901 cattaccgga ggcgctggta atcaccaccc attttaatgt gtctgaaagc agactttttg
    54961 cggcagcaat ggcactgtcg agatcgcggc aatttttacc ggtcaggatt tccagctcaa
    55021 agatattggg ggtaattccc tgcgccagcg gcagtaaata ttgtcgatac gcctcgggaa
    55081 ggtcaggttt gacataaatt ccgctatcaa tatcgccaat caccggatcg accatgatca
    55141 ataggtcagg atggtctttg cgtagcgcag tcagccactc ggcaaggatt ttgatttgcg
    55201 atgccgttcc catatagccc gtggttacag cacgaagttg gcgcagcgca tcacgctcct
    55261 gaagcgcacg caaatagccg ctaaaccatt cgtccggaat cgcaccaccg tagaaagtgt
    55321 cataatgcgg cgtattgctc agcaataccg tcggcacggc aaagacattc aagccgttct
    55381 gttttatagc aggcaccgca atgctgttgc ctacgcttcc gtacactacc tgcgactgca
    55441 cggcgacgat atccgcctgc agcgccctac tcttatcgtt aaacaacaac aaactactca
    55501 tttaattttt tctccttgcc gatgatcctc atcgtattcc aaccgaaact ttacctgatt
    55561 ctggcagtca aatcggctat tacaaaacaa agataaggta attcagtgaa gaaaatcatt
    55621 tgtctggcca ttacgctgct aatgacactc cccgcttacg cgaagttaac tgttcatgaa
    55681 gaagcccgca tcaacgccat gctggaggga ttagcacaga aaaaggattt gatatttgtg
    55741 cgcaacggtg atgaacatac ctgcgatgaa gcggtttccc acctgcgtct gaagctcggc
    55801 aatacccgta accgcattga cactgccgag cagtttattg ataaggtagc ttcgtcgtca
    55861 tcgattaccg ggaagccgta tatcgtgaag atccccggta agagcgatga gaacgcacag
    55921 ccttttttac atgcgttaat tgcgcagacg gataaaacgg tgcctgcgga aggaaattaa
    55981 tccgctttgg taaggcattt acaggaggta acatgaaaaa atgctttatt tatcacgatg
    56041 aaaaatcgaa taaattttgg tggatagatt acgaagggga tagtttagct gtcaactatg
    56101 gcaaggtagg tagtattggt aaattccata caaaagaatt cgataatgaa gaacagtgtc
    56161 tgaaagaagc cagtaaattg attgccgcaa aaatgaaaaa aggatatcaa gaaactccag
    56221 actttaactt catggatctc tactattttg atgatgaaga gattgggtta catcttaaaa
    56281 cgtcacaccc aaacttccag tgccatttta ctgatccact ttatatgtgt tgctgggatg
    56341 aagaatctcc ttttggtagc gatgaaggtg ctgatgctct aaacgaactt gaaaatagcc
    56401 tccgttctgg actgtgctga tttccctcaa atgttaattg aaactatatg gggtatgaaa
    56461 tacatcgcta tggacagtat tcttgaagag gatgtgcgtg cgcaattact agccgatgaa
    56521 atgagctcta tccagagcaa tatgattacc tacgcgactg cattcggtca gatcaaagtc
    56581 atgggtaaaa tctcccataa acttaaaaag atgggactca atgcactagc gcgtcatcag
    56641 cttaccgcaa aaattcttca atggggtgac ggtcaggact caccaatact tcaaaaaatg
    56701 attgatgacc ttactgcttt tccacacgaa aattaaaaac tacatttgtc ggcagcaaca
    56761 actgccgaca aatcagatta ttgacaaagt gcactttgtt tatgccggat gcggcgtaaa
    56821 cgcctgatcc ggcataaaaa tattgctaat tcaatatatt gcagaaacat tgtaggcctg
    56881 ataagcggag cgcatcaggc agttttgcgt ttgtcatcag tctccaatgc tattaatcct
    56941 taaatccctg ccccctgact aaaatgctct tccccaaaca ctccggtgga gaggtagcga
    57001 tcgccacgat cgcagatgat cgctaccacc accgcaccag ggttagcttt tgccacccgc
    57061 agtgctccag caaccgcgcc gcctgagctg acgccacaga atattccttc ccgcaccgcc
    57121 agttcgcgca tggtgttttc cgcatcgcgc tgatgaatat ccagcacctc atccaccagc
    57181 gcagcgttga aaatccccgg cagatattcc gcaggccagc ggcgaatgcc cggaatactg
    57241 ctgccttctt ccggttgcag gccaacaatg gtcaccggtt tggattgttc gcgcataaag
    57301 cgtgaaacgc cggtgatagt gccggtcgtc cccatgctgg agacaaaatg agtgatgcgc
    57361 ccgccggttt gctgccagat ttccggcccg gtggtggtgt aatgcgcata agggttatcg
    57421 ggattattga actgatcgag cagctttcct tcgccacgat tcgccatctc cagcgccaga
    57481 tcgcgcgcac cttccatgcc ctgctctttg gtgacaagaa tcagttccgc accataagca
    57541 cgcatcgccg cacggcgttc ctggctcatg ttgtcgggca tcagcaattt catgcgatag
    57601 cctttcagcg cggcaatcat tgccagcgca atgccggtgt taccactggt ggcttcgatt
    57661 aagacatcac ccggtttaat ttccccgcgc ttttccgcct cgacgatcat cgaaagtgcc
    57721 gcacgatctt tcaccgaacc tgccgggtta ttgccttcca gttttaacca cacttcactg
    57781 ccgttatccg gccccattcg ctgcaacttc accagaggcg tattgcctat tgtttgttct
    57841 aatgtactca cgatctctat ccatacgtgg tgttgcctga tgcgacgctt gcgcgtctta
    57901 tcaggcctac aggttacaaa ccttgccatt aaaaagcccg gatcgcggga agcgcctccg
    57961 ggcgtttaac attcactcaa cctatcaggc gctttgtgcg agagcaagtt cctcatcgcg
    58021 ggtttcaata cgctcgtcgc cgttatacag ccgcgcatgt tgcagaccaa cgaataaacg
    58081 ctcgccacgc tgcggggcat cgtcgccatg catcacgacc gtcagcggtt cgttgtacca
    58141 ccccagcggc tgcaccacta attgggtgta gtgacctttc gggctggctt ccagtacctg
    58201 taccggcagc ggcgaatcga gactggtacg gcggctgata tccacttccc acgggcgcag
    58261 gaagagatcc accggcccct gatacgcagg tgtgtagccc agcggccagc gatgcgcgcc
    58321 aacatggaac tgcccgccgc gaatggttcc ctgcaggcgg ttcacttcgc ccataaattc
    58381 gagtacaaaa cgggtcgctg gttcgcgcca tacctgattc ggcgcatcag cctgttcaat
    58441 attcccctga ctcatcacca ctacacgatc ggctacttcg gtcgcctctt cctggtcgtg
    58501 ggtcacaaac acgctggtga atttcagctc ttcatgcagt tgacgcagcc agcgacgcag
    58561 ctctttacgc acctgcgcat ccagcgcgcc aaacggttca tcaagcagca gaatttgcgg
    58621 ttctacagcc agcgcgcgcg ccagcgccac acgctgtttc tggccgccgg aaagctgcgc
    58681 cggataacga tccgccagat gcgcaagctg gaccatttcc agcaattttg tcactttcgc
    58741 tttgatggct gcggcattcg ggcgctcgcg acgcggcagc accgtcaggc caaaagtgat
    58801 attgtcgaat accgtcatat ggcggaacag cgcgtaatgc tggaacacga aaccgacttt
    58861 acgatcgcgc gcgtgcaggc gactcacgtc ggtgccgtgg aagcgaatat gcccgctggt
    58921 ttgatgctcc agcccggcga taatccgcag cagcgtggtt ttcccggaac cggacggccc
    58981 cagcaacgcg accatctgac cagaaggaat atccagtgag atatcgttca gcacctgggt
    59041 gcgaccaaaa gacttcttga tattggcaat ctcaatgctc atgatgctcc tcctgctgcg
    59101 cgcgtttttc ctgattctcc aggcgccact gcaacatact ctttaaaaac agggtgataa
    59161 tcgccatcag cgtcaacagc gcggcggcgg taaaggagcc gacggtgttg tagtcctgct
    59221 ccaacaattc aatctgtaac ggcagcgaaa gtgtttcgcc gcgaatcgag ccggaaacca
    59281 ccgacacagc accaaactcg ccaatcgcgc gggcgttggt cagcaccacg ccgtaaagca
    59341 gcgcccagcg aatattcggc aatgtgacgc gacggaacat ctgccagccg gacgcgccaa
    59401 gcaaaatcgc cgcttcgtct tcctggctgc cctggcttaa catcaccggc accagctcgc
    59461 gcaccacaaa cggacacgtc acaaagatgg tgaccagcac cattccgggc caggagaaca
    59521 taatttgcag attatgttca tccagccagc cgccgagcgg gccgttggag ccgtagaaca
    59581 gcagatacac cagaccggca accaccggcg atacggcaaa cggaatgtcc agtagcgtca
    59641 gcagtaactg gcgtcccggg aagttaaagc gcgtcaccag ccaggccagc agaatgccga
    59701 acaccaggtt taccggtacg gcaatcagcg cgatcatcac cgtcagccag atggcgtgca
    59761 gcatgtccgg atcggccaga ttctgtaaaa ccggcatcag ccccttgctg aatgcctgca
    59821 cgaagatgta aatcatcggc accagcagga tgaacgccga aaccagcatc ccgatgccaa
    59881 tcagaaacca tttgccccag ttaatcgggc gcgcgtcata acgcttcaat tgggtaactt
    59941 ccgccattaa tgacctacca cacgccgacc aaagcgactt tgcagagtgt taattgagaa
    60001 cagcagcagc agagatgccg cgaggatcac cgaagcaatc gcgctcgctg ccgggtaatc
    60061 aaactcctgt aagcgcacaa aaatcatcag cgacgtcact tccgtcttcc acgcgatatt
    60121 tccggcgata aaaatcaccg cgccaaattc accaagacta cgggtaaacg acagcgccac
    60181 gcccgccacc agcgccggag aaagctccgg cagcaccact ttgcagaaac tctgccagcg
    60241 cgttgcacca agcgtttccg ccgcttcttc atattccggg cctaactctt ccagcaccgg
    60301 ctgcacggta cgcaccacaa acggaatgct ggtaaaggcc atcgccaccg caataccaag
    60361 ccaggtatag gtgactttga tatcaaactt cgccagccat tcaccgtaaa aaccgtttac
    60421 ggaaaagagc gaggccagcg ttaaaccggc gactgccgtt ggcagcgcaa agggtaaatc
    60481 catcagcgca tcaagcagcg tgcggcccgg aaagcgataa cgtgttaaaa tccacgccat
    60541 cagcagaccg aaaacgccgt taaaaatcga tgccacaaac gccgacagca gcgtcacttt
    60601 gtaggccgcg accacctgcg ggttggtgat cacctcccag tactgcgccc agctcatctc
    60661 agaaagctgc atcaccagcg cagagagcgg cagcagcaat atcaggcaca caaacagcag
    60721 actggtgcca aggcttaaag taaagcccgg cagcacgcgt cttgaagaaa ctgcaaacat
    60781 tacttacgcc ccgccgctaa cagcttgtct aactcgccgc cgctggtgaa gtgggttttc
    60841 atcacttccg gccatgagcc aaatttgtct tccacgcgga acagttcggt ctgcgggaat
    60901 ttatctttca gtttgtccat gacttccggg ttattcacac ggtagtaata gtcggtgatg
    60961 atggtctgcg cctgtgggct gtagagccag ttcaggtagg cttttgcggc tttttccgta
    61021 ccgttggcct gcacattttt atcgacccac gccaccggga attccgccag aatattggtt
    61081 ttcgggatca ccacttcaaa gccctgtgct tcatactgtt tacggatgtt gttcacttcc
    61141 gattcaaagc taatcagcac atcgcccaag ccacgctcgg caaaagtggt ggttgcgcca
    61201 cgaccgccgg tatcgaacac ttcaacgttt ttcaggaact gggtcataaa ctgttcggtt
    61261 ttggcttcat cgccaccgtc agctttatcc gctgcgcccc atgccgccag ataggtataa
    61321 cgcgcgttac ccgacgtttt cgggttcggg aaaatcagct tcacgtcgga gcgcaccagg
    61381 tcgttccagt cgtggatatt cttcgggttg cccttacgca ccaggaagcc catggtggag
    61441 tagaacggcg agctattatt cggcaggcgc gactgccagt cagccgggat cagcttgcct
    61501 ttgtcgtgca ggatttgtac gtcggtcacc tggttataag tgacaacgtc tgcttttaag
    61561 ccctgcaaaa tcgccagcgc ctgttttgat gacccggcat gagattgttt tatcgtcagt
    61621 ttgtcgccgc cgttatcttt tgcccattgc tgttcaaacg gcggattcag ggcggcaaac
    61681 agctcgcggg agacgtcata agaactgttc agcagttccg ttgcctgtac atggcccgcc
    61741 agcagcagag aagcgaccag cgcgagtgag ttctttttca gtaagttaac ggccattgcg
    61801 cacccttata aatttaatga ctttctaata gccatcatat ttataacgga cgtgaaagga
    61861 gtaacgattt tatataccgt ttggtgattt ggaagttaaa aagggaataa gaacttggtt
    61921 caacaggcgt aattgttgca ggcagtttgg acacagacag cgcggagaaa ccggagcgta
    61981 cacatagtac gtgaggattt cgagcactgc ccggggccaa aatgacaaat gaaatagcct
    62041 ggtgaactta gttcaagacg aaatcctccc cacacatgca gggaggaaac agaggtgaat
    62101 cagataccga cgctaaccgt ctccggcagt gtgctgccgc catcaatcac attctgtgta
    62161 ccggttaaat agctggattc atccgatgcg aggaaggccg ccagttcgcc gacttccagc
    62221 ggatcggcga ggcgacacag cgggattgct tttgccattt cagtcagcac cgattctgga
    62281 tcttccgggt tcgactggcg ggcaatgctt tccgccattg gcgtgcggac gtatcccggg
    62341 cagatggcgt taacgcgaat accggattgc gcgtactcca ccgccagcga tttagtcagg
    62401 ccaacaatcg ccgctttcgt taaggcatac gccgtttcgc caggatcggc caccatatca
    62461 ccagtgactg aagacatcat cacaatgcga ccatctttgc gcgcaatcat ctccggcagc
    62521 accgccttcg tgacgttcca tacgccttta atattgatat caatatggaa atcgcgatct
    62581 tcatcgctca tatcgaggaa actgcccaga cgacaaacgc ctgcgttatt cactaggata
    62641 tcaatgcgcc cttctttttc cttcgcgcgt ttgatagctg cggctaccga cgccgggtca
    62701 cgcacatcgg cgacaaccgc cgtacagcga tgaccacgac cacacagttc gtccgctagc
    62761 ttttcgatct caggggagat atccagcaag attaggttcg cgccatgacg tgcaaaagtt
    62821 ctggcaattc cttcgccaat tccctgcaat gcgcccgtaa tcagtgctgt cttgcccgtg
    62881 agtttaccca ttttaatgct ctccttgtta atacagagcg ttacactcca ttccctctga
    62941 aaatacagga taaacaaaat attgctgcgg atcagctcac taattcagga aaatctcaat
    63001 ccagtcgctg ctttatgcat catttcaggc gttgtgttag ctcactgctg cgctgaatca
    63061 ttttcaatga cgccacatca ttgatgttgc acaagaccaa caaacagtaa atcggtaaca
    63121 gaattttgcg ccgtgcgggt agacatcgac gagctacgcc attcggtttc accggaaacg
    63181 gtatcgagcg tgaaatgcgc cagccgccgc agcggagagt cagccagaga agtaatggca
    63241 atcaccgttg ccccctgctt tcgtgccgct tctgcgcaca agacaatctc ttttttactg
    63301 ccgctgtaag agatagcgat ttgtacatcg ccttttttca acgcctggga aacggtggct
    63361 tgtacgtgcg tgtcggcttc gcaggccacg cgatagccaa ttttcatcag tttgaacgac
    63421 agatcgcgcc ccaccagcgc cgatccgccc aggcccgtta tctggataaa cggtgcttta
    63481 ctgatgacct caatgatttt ttgcagccgc gcgtagtcga acaacgcaca ggtttgctcc
    63541 agcgccagct ctttttcgcg attcagtttg cgggcaatca cttccagcga atcatcactg
    63601 gtaatcgaac tgtgcaggtg cagcgccgtg gcattcgttt tctcccggct ggcactgtat
    63661 tcgccaatta acgccatacg caattcagta aatccttgcg cgccgagctt ctgggcaaat
    63721 ttcacaatgc tcgactggct gataccgagc tgtttcgcca tctgccgtga agagacggat
    63781 ttcagctcgc tcactctggc ccgcagaaag tcagcaattt tttgctcgtt cccggtaaat
    63841 tcactttccg cattgcgtat tttcgtcagg tacaacatct tccactctcc tgggctggca
    63901 gcaaaaattc cgcttaagtt cttatgacgc tcttcacact ctgcgagtgt agactcaatg
    63961 attcctttaa gtctgtcaat tcgggatata tgattccata catctgcaat tatttgatcg
    64021 taaatagtaa ggtcaccacc gatgcaacta gaaaagatga ttactgaagg ctcgaacacc
    64081 gcctcggctg aaattgaccg cgtatcgacg ctggaaatgt gccggattat caacgatgaa
    64141 gataaaaccg taccgcttgc cgttgatcgc gtcctgccgg atatcgccgc ggcgatcgat
    64201 gttatccacg cccaggtcag cggcggcggt cgtctgattt atctcggtgc gggaacatcc
    64261 ggtcgtctgg ggattctgga tgccagcgaa tgtccgccca cctacggcgt gaaaccgggt
    64321 ctggtggttg gtttgattgc tggaggcgaa tatgccattc agcacgcggt ggaaggcgct
    64381 gaagatagcc gggaaggtgg tgttaacgat ctgaaaaata ttaatttaac ggcacaggat
    64441 gtggtggttg gcattgctgc cagcggtcgc acgccgtatg tgattgccgg actggaatac
    64501 gcacgccagc tcggctgccg cacagtggga atttcctgta atccggggag cgccgtttca
    64561 accaccgctg agtttgccat tacaccgatt gtaggtgccg aagttgttac cggttcttcg
    64621 cggatgaaag caggcacagc gcagaaactg gtgctcaata tgctttccac cgggctgatg
    64681 attaaatccg gcaaagtatt cggcaacctg atggtcgatg tggtcgccac caacgaaaaa
    64741 ctgcatgtgc gccaggtcaa tatcgttaaa aacgccaccg gatgtaatgc cgaacaagcg
    64801 gaagcggcgt taattgcctg cgagcgcaac tgtaaaacgg ccattgtgat ggtgctgaaa
    64861 aatctcgatg ccgcagaagc taaaaaacgc ctggatcaac acggcggctt tattcgtcag
    64921 gttttagaca aggaataacc catggccaaa gagatcagca gtgaacttct gaacaccatt
    64981 cttacccgtg tcggcggacc gggaaatatc gccagttgtg gtaactgtat gacgcgcctg
    65041 cgtctgggtg tacatgacag ttcactggtt gatcccaata tcaaaacgct ggaaggcgtg
    65101 aagggcgtca ttttgaccag cgaccaggtg caggtcgttt ttgggcctgg taaagcgcat
    65161 cgtgccgcaa aagcgatgag cgagctgctg ggcgaggcac cggtacagga tgccgcagaa
    65221 atcgccgccc agaacaaacg tcagttaaaa gccaaacaaa cctccggcgt gcaacaattt
    65281 ctcgccaaat tcgccaccat cttcacgccg ctgatccccg gttttattgc cgccggtctg
    65341 ctgctgggga tagcgacgtt aattgccacg gtgatgcacg ttccggcaga tgctcagggc
    65401 acgctacccg acgcgctgaa ttttatgaag gtgttcagca aaggtttgtt caccttccta
    65461 gtgattctgg tgggctataa cgccgcccag gcatttggcg gcacgggcgt caatggcgca
    65521 attattgccg cactcttttt gctcggttat aaccccgctg caaccaccgg ttactacgcc
    65581 ggttttcacg atttctttgg tctgcccatc gatccgcgcg gcaatattat cggcgtgctg
    65641 attgccgcga gggcctgcgc ccgcattgaa ggcatggtgc gccgctttat gccggacgat
    65701 ctcgacatgc tgctgacctc gttaatcacc ctgctgatca ccgctacgct tgcgtacctg
    65761 atcattatgc cgctgggtgg ctggttattc gaaggtatgt catggctgtt tatgcacctg
    65821 aacagtaatc cgttcggttg tgcggtttta gccgggctgt tcctgatcgc cgtggtattt
    65881 ggcgtgcatc agggctttat tcctgtttac ctcgcgttaa tggacagcca gggattcaac
    65941 agcttatttc ccatcctttc aatggcaggc gcgggccagg tgggcgcggc gctggcactc
    66001 tactggcggg cgcaaccgca cagtgcgcta cgcagtcagg tacgcggggc gattattccc
    66061 ggcctgctgg gcgttggtga accgctgatt tacggtgtca ccctgccccg catgaagccg
    66121 tttgttaccg cctgtttagg cggcgcggcg ggcggtttgt ttatcggatt gatagcctgg
    66181 tggggtctgc cgatgggctt aaacagcgcc tttgggccgt ctggtctggt ggcgctgccg
    66241 ctgatgactt ccgcacaagg catccttccg gcaatggcgg tttatgctgg cgggattctg
    66301 gtggcatggg tttgcgggtt tattttcacc acgctctttg gctgccgtaa cgtcaatctg
    66361 gactgatatt atgaaacgga caatgctcta tctttctctg ctggctgtta gttgtagcgt
    66421 cagcgccgca aaataccctg ttctgacaga aggtagttga tcaagtatat gcggcgttaa
    66481 agcagtaata aaaaggccag tcagtaatgt tactgcctgg cctttaatca atgaatcaga
    66541 aacgcttaca gcaccatcaa cttgtccagc gacggtgcga aataatagcc gccggttacc
    66601 ggtttggtga aacgcaacat cgcatcacgt ttgccatcgg tatcgccaaa catgctcagc
    66661 agttgctgct caatgttatg cagacgcgcg cagtaggcgc agaagtacag accgtgagtg
    66721 ccactggcgg tgccgtacgg caggctctgg cgaacaatct tcagcccttt gccatcttct
    66781 ttcagatcaa cgcgggtgag gtgagaggtt tccggacgtt cgtcgccgtc aatctcttcg
    66841 ttggcctctt tggtgcgccc gatcatcatc tcctgatcgt gaacgctcat ccggttgagc
    66901 tgcttcaggt tgtgctccca acgctgcaca aacacatagc tgccgccggc atccacgccg
    66961 tctttgataa ccgccacttc gcggcgcgtc tcttcacccg ccgggttttc tgtaccgtca
    67021 acaaagccgc tcaggtcacg ctcttccacc caacggaagc cgtggatctc ttctttcact
    67081 tcaatgcagt caccaaaggc ttccatcgcc gcctgagcga cagagaagtt tacgtcgtga
    67141 cgcagagaaa gaatgtggat caacacatcg aactgggtcg tcggcgcaag gcctttaccg
    67201 tagcccggaa aatctttcag ctcttctgcc ccaacgccgc cgctcagagc gcgccaggtg
    67261 ttgttaccaa aggcaaccac cgcaccaaga tgcgcgtccg ggaatttcgc ttcaaaagtt
    67321 gccagtttgt cggcaaatgt tttactggcc gcacgcaggg cgtcaacttc ccctttcacg
    67381 ttggcttcga tccaaatcgc cgcgcggcaa tgttctggca aaatgccact ctgaacctga
    67441 gacattgttc ctcctgaaaa taagaatgcc acgcaatccg tggcattgtt gagcgctatt
    67501 ttacgtgttt tttcgcgacg gcggtttgtt cagacgcaaa ttaacgccgc cagataattt
    67561 tactgacttt ccagtttttc agggtatcgt ccgaaggcat taacccttca ggaccacgcc
    67621 attccccgct gaactggtag ctaatatgtt gactgccttc ggctttacat tcgacggcac
    67681 gattatcatc accatcagct ttttggcaat tgccaaatgc tttgctgtaa aggtcgctaa
    67741 acggtgtacc gattttaaca ccggtgtcag caggaatatc gctatccagc acgtcaatgc
    67801 gactgatcgt gccttgatcg ccgttaatca ccatcgccac gttgtcgcct ttcatcactt
    67861 caaaaaagcg caccacgttg ccgttcgcgg ttttcattcc gctgcgcagg cgataatcgc
    67921 catcaagcgc atcagcaatg gcttgttctt gcagtggtgt ggacgccgtt aattcaccca
    67981 cgccctgctc gctcactttg gtggacgatc caaaccagtt ccacgggtta gcggcagacc
    68041 agttaactga cgacatcgtg gaacagccgg tcagcatcaa cggcatagcg cataacatta
    68101 aacgcagcga tttcatgtca cttcctttgg ttattcaata acgttgcttg gagtgcaaat
    68161 tcaccaaaaa gtgccgttat tctttttctt cattaaaaca agcgcgtaaa cgtcgattgg
    68221 tcagcagcca gattaacgcc acaatatccg ccactaccag cgccagaccg ataccgctaa
    68281 cggattcacc gttcagccac agccacggtt gccagcaaag taaaaccacc tgcgccaaca
    68341 gcaacagaaa atagagcaca tgccaggtgc gaggaaacga agcccgccga ccgctcaaca
    68401 aaaacgccag caccgccgga ataccgggaa tcagccccag ccagaaatta tcgtgatcgg
    68461 gataaaacag atttagcagc gcagtaccct gctcgcgcga cgcaccggca atgacaaaca
    68521 gcacccaggt tcgcgcctga agcaatagca caagccagaa gagcaagggt aaacgcaggc
    68581 gtccgtgcgc atcataatgg acaggatgga actcagtact cttcatcttc aatcaaacgc
    68641 ttacccagac taagcacgtc ggcgtgttca tatcccaggc gttcatacat tccgagcacc
    68701 atgtcgttat cttccggcac gttgatctga attttcgggc agccgcgagc aatcagcttt
    68761 ttctccagcc ggttaagcaa cgcattggca attccacgcc cacgaaactc tggatgcacg
    68821 ccaagataat aagcagaccc gcgatgcccg tcataaccgc ccatcaccgt tccgaccact
    68881 tcaccgttta cctcagcgac caaaaacaaa ctgacgtcat ggttcatctt acgctcgatg
    68941 tccatttccg gatcgttcca cggactcagc aagtcgcaac gctcccaaag ggtgatgacc
    69001 tcttcgaaat cttcctggcg aaatacgcgt atctccatgg tattcgttac ctttttgcgg
    69061 gttaaaaggc tgattatggc gtgaacggtc gaattagcca atatctgacg aaaatcggtt
    69121 gaaaaagtgg cataatgggg agttgtcaac tattgaaatg aaaagtaaaa caattctcaa
    69181 cagcaaaccg tcgtaacgga ttacgcgata cgatataaca tctggaactt tattattaca
    69241 actcaggccg tatgagcact tttaaaccac taaaaacact cacttcgcgc cgccaggtgc
    69301 tgaaagccgg tttagctgcc ctgacgttgt caggaatgtc gcaagccatt gccaaagacg
    69361 aacctttaaa aaccagcaac ggacacagca agccgaaagc caaaaaatct ggcggcaaac
    69421 gtgtcgttgt tctcgatcca ggccacggcg gaattgatac cggagcgatc ggacgcaacg
    69481 gttcgaaaga aaaacatgtg gtgctggcga ttgctaaaaa cgtccgttcc attttgcgta
    69541 atcatgggat tgatgcgcgt ttaacgcgtt ctggcgatac gtttatccca ctttacgatc
    69601 gcgttgaaat cgcccataaa catggcgcag atctgtttat gtcaattcat gccgatggct
    69661 ttaccaaccc gaaagctgcc ggtgcttcgg tatttgccct ctctaaccgt ggggcaagta
    69721 gcgcaatggc gaaatacctg tctgaacgcg aaaaccgcgc cgatgaagtt gccggtaaaa
    69781 aggcgactga caaggatcac ctattgcagc aagtactgtt tgatctggtg caaaccgata
    69841 ctattaaaaa cagcctgacg cttggctcgc atattctgaa aaagattaag ccggtgcata
    69901 aactgcacag ccgcaacacc gaacaagccg cgtttgtggt gttgaaatcg ccgtcggttc
    69961 cttcggtgct ggtggaaacc tcgtttatca ccaacccgga agaagagcgg ctgttaggca
    70021 cggcggcgtt tcgtcagaaa atcgccacag cgattgctga aggcgtgatc agttatttcc
    70081 actggttcga caaccagaaa gcacattcga aaaagcgata agttatgaaa cccgacgcac
    70141 accaggttaa acagtttctg ctcaaccttc aggatacgat ttgtcagcag ctgaccgccg
    70201 tcgatggcgc agaatttgtc gaagatagtt ggcagcgcga agctggcggc ggcgggcgca
    70261 gtcgggtgtt gcgtaatggt ggtgttttcg aacaggcggg cgtcaacttt tctcacgtcc
    70321 acggtgaggc gatgcctgct tccgccaccg ctcatcgccc ggaacttgcc gggcgcagtt
    70381 tcgaggcgat gggcgtttca ctggtagtgc atccgcataa cccgtatgtt cccaccagcc
    70441 acgcgaatgt gcgttttttt attgccgaaa aaccgggtgc agagcccgtt tggtggtttg
    70501 gcggcggctt cgatttaacc cctttctatg gttttgaaga agacgccatt cactggcacc
    70561 gcaccgcccg tgacctgtgc cagccatttg gtgaagacgt ttatccccgt tacaaaaagt
    70621 ggtgcgacga ttacttctac ctcaaacatc gcaacgaaca gcgcggcatt ggcgggctgt
    70681 tctttgatga tctgaacacg ccagatttcg accactgttt tgcctttatg caggcggtag
    70741 gcaaaggcta caccgacgct tatttaccaa ttgtagagcg acgtaaagcg atggcctacg
    70801 gcgagcgcga gcgcaatttt cagctctacc gtcgcggtcg ttatgtcgag ttcaatctgg
    70861 tctgggatcg cggcacgctg tttggcctgc aaactggcgg gcgcaccgag tcgatcctga
    70921 tgtcaatgcc gccactggta cgctgggagt atgattatca gccaaaagat ggcagcccag
    70981 aagcggcgtt aagtgagttt attaaggtca gggattgggt gtaactccct cacccccatt
    71041 cccgcatccg ctgatgcaac gtcaacgacg gcttctcggc aaacagctgc tggtaatccg
    71101 tggcaaattg ccccagatgc cagaatcccc actgcatggc ggcgtctttt accgttgtgc
    71161 tttgcgacca cgggcttatc agttcgcggc gtacggcgtt caggcgaatg cgtttcagcc
    71221 acgcgtttgg gccaatgcct aaaatagcgt gaaacgcgtt ttgtagcgtg cggcgactga
    71281 catgcagttg attacacaaa tccagcaccg tcaccggttc ggacatgttt tccagcacat
    71341 attcacgggc gcgggaaagc aaccgacggt aactctgatg actgatgctt tccgccgtca
    71401 ccattggttg cgcttcttcc agcatcgccc ccatcgccat tagcaaatta tcccccagca
    71461 cttttcgcac tgcaggctga tggagatttt ccgggttctc gcaaaacgtc gccagcgcct
    71521 gttggacaaa gccccacagc gcggctttat gctgcttctt tcacttccag cgccgactgg
    71581 ctacgcagca tatgtaatac ccgatccgga ttgtgcaaaa agttagcctg acaggtgatg
    71641 acatcttcag acagcactac gcccaggatc gtataatcgt ccggcgtact taattcaaac
    71701 tcggttccac cggggcgcgt tgctatttct gcgcttccca gacattgcga accgataaat
    71761 ccctgctcac cgcgcgtcgc cggaatgcca aaccagaacg agttcggcca gaccaggcac
    71821 gactgacgca gcgccagacc ggtgtattca cgaaaaacct gaatatcatc gagtagaatt
    71881 tccgtaaatt caccatgaaa cttgcccgga tgcagctgat cgtaaatctg ctgccaggcg
    71941 gtaatcgtca aagcatgttc atagacatcc gttgtctgtc gttgatgaac attatccact
    72001 tcgaccttcg gcgtgagctt caggttttcg ggtaagggtt catgataaag atggtgcaaa
    72061 ttggctgtac gggtcttttt catgatgtta atgccgggtg ttgtaggaca cccggcacct
    72121 ccgacaggtt aatggggctt gagacgataa cgactactgc gtttacgtta cgtcccggca
    72181 gaaaagagct gttcgagcgc atttctggct ttttccagcg gccagccaaa gtgggcggcc
    72241 acttctcctg ccgtcattcc ctgacatact gatgttaaca gcgccaacaa ctcgtcagca
    72301 gattccgcga cgataactgg cgcgtcgggt tcctttacgg gttgcttcgg ctggcggtta
    72361 aagccagtaa ccagccactg ggtgtcatcg tctggccgac cgatctcctt acggctgatg
    72421 acacgcccgg tacgcatcgc ggcagcacaa cctgcgtcca gcgccgcacg acacgccgcc
    72481 agatcgcctt ccaccaccag cgttaagcga ccagggtcaa gcacttcgtg actgagcaga
    72541 cgaacgttag ctgctttaag catggcatcc gctgcatcta tcgcggcgac catgccgtcc
    72601 acttccagca atcccagtgc attgatcatc ggcaacctcc gttacgcacg ctggattgga
    72661 ttacgcgcga tttccagcac tgcatcggta aaggcgttac aggctgcttt acacgcggcc
    72721 tggctaccgg ttaaaaatgc tgccgagtag ttggtttcag acggtggcgg gacgtaggtc
    72781 accagctgta cgtcggcaga tttcaacgcg gcatcaatgc cgtaggtcgc ttccagcggc
    72841 ggtgccacca gatacgccat cggatcgcca agcgtgatcc cggcggttga tgaaagataa
    72901 gaacctgtac gcgaaaccac atgcgccagg aaggcggtgt tttctgcgtc attggcccac
    72961 tggaaagctg cgccattttc aatatgtgca accatcgcat ccagaccggc acgcacttcc
    73021 gccgggtttg gcccaccaag cataatcagc acttcaccgg cagtcggtga cgggccgtgt
    73081 gccgctcccg catacagcga gcgaccatat accacttcga ccatcgcttg tttggtcgct
    73141 tcgtcagccg caatgtaggt tacgtcgtca gaatcggcag aaatcagccc gaggctacga
    73201 atatgcggcg gcaattttag ttcacgcgca aattcagcgt taacggaggc aatcacccgc
    73261 atggcggtta ccgacggtcg aatcaaatct aaagctggca tgatgcctcc ttatcgggtc
    73321 atgttgatgc cggatgcttt ctgctccagc atacgtttcg ccaaatccac aatgacggct
    73381 gcggcttcaa ccggcggcgt gccgccctgg tgaatgttag agatacaggt gcgatcggcc
    73441 tcaacggtgg tcgccatacg cggcgagtaa acggcgtagc aggagaggct ttctgactgc
    73501 cctaaccccg gacgttcccc caccagcagg atcaccactt tcgcgccgag gatctcaccg
    73561 atctgatctt caatcttcac gcgaccataa cgaacaaaga atggcgtgcc gactttcagc
    73621 ccggcctgtt tcaggcccgc catcagcggc ggcaggatct cttcgtagtt gacggtgatg
    73681 gcatcagttg acaggccatc agaaataacg acctgtacgt ccggattagc aacacactgt
    73741 gctttcagcg cttcaacagc ttctgcacac aggcggcggc ccatatccgg gcgagtcagg
    73801 tagagatttt tgtcgctaat ttcagagcgt acttccagca agccctgcgc tttcacccac
    73861 tcttccggca cttctttcag tacggtatct ttcgaacgag agtgatcggc caagaaacgc
    73921 agcagcgcct gagtacgtgg acgcggaccg gcacgaccgg tacaaacgcg ggccacggtg
    73981 ctgcggcgca gttctgttaa tacgtctgcg cgatgcgggt tttcaacacc aatccacgct
    74041 tttgcttcag cggaacctaa atccagcgca cagctttccg aggtcaccgg tgccgcacag
    74101 ttggtggtgg cgcactttgc ttctgacggc gcgggggccg cttgtcccat tgacgccatc
    74161 acgctgcgta caatttcttc aatctgtttt tgatccatga tgtgttatct ccgcgtcatc
    74221 agaagaacag tgacggatcg cccgcccgtt tggtcaggcg accgtttgcc ataatgccca
    74281 tgctttccag ccagcgttca aactctggtg acgggcgcag gttgagtaac tgacgcacag
    74341 tggaggtatc gtggaatgcg gtggtctggt agttgagcat gatgtcatca cccagcggca
    74401 tccccatgat gtagttgcag cctgcggtgg cgagcaggat catcaggttt tcgttgaggt
    74461 tctggtcaga gtcagcgtgg ttggtgtagc agccgtcaca gcccatagag atgccgctca
    74521 gcttgcccat aaagtgatct tctaagccag cacggataat ctggcggtcg ttgtagagat
    74581 actccggccc aataaagccg accacggtgt tgacgataaa cggatcgtaa tgacgcgcca
    74641 gcccatagtt acgtgcttcc atcgtcacct ggtcagcgcc gaagttagcg ccagcggata
    74701 gcgcagagcc ttgtccggtt tcgaagtaga ggcagttttc cccggcgata cgattgaact
    74761 ctgcgcccac tgcgcgcgct tcgtcgagca tcgccagttc cacgccaaac tctttcagcc
    74821 ctttttcgct gccacagata ctctggaaaa tcagtccgcc aggagcgccg cgacgaatcg
    74881 cttcgatctg ggtggtgacg tgcgccagta cgcagccctg agttgggatg ttgaatttgt
    74941 cgatcacgcc ataaatggta tccagcacgc ggcttaagtt ttccacgtcg tcagttaccg
    75001 ggttaacgcc gatcaccgca tcgcccaccc cgaaggaaag cccttcatag atttgcgcag
    75061 cgatactttg cacgtcgtca cgggtatcgt tcggctgcaa acgggcgcta aaggtgcccg
    75121 gaataccgat ggtggtattg gcctttttga ttaccggcat tttcttcgcg ccgtagatca
    75181 ggtccgcgtt ggagcaaatc ttcgctatcg ccgcgaccac ttccgaggtc agccctttgc
    75241 gggtaaaggc aatgtcgtcc acgctggttt catcgctcag cacatactca cgcagttcgc
    75301 tgatgctcca gtttttaatc tggttgtagg ccgtttcgtt aacgtcgtcc tgaatcagcc
    75361 gcgtcacgca gtcatcttca taggcaatca ccggattatt gcggatgtcc gctacggtca
    75421 tttccgacaa cacctgcttt gccgccacgc gctcctgtga gcttgccgct gcaacgcccg
    75481 ccagcacatc ccccgaacgc agttcgttgg ctttagccag cacctctttt acatccttaa
    75541 actgatatac attgccgaac aatgtggtct ttagtttcat aagtcgttcc ctcaggaaga
    75601 agtgatgagt aacatttctc acagcctgca aacaattcta tttcatcgtt agcccttgtc
    75661 atgtaaggtg atttccttaa cacgcccgtt acttatcctg ccgaacgcaa ccgaactcgc
    75721 cggatacatg agcggggtta tcgagtcgtt agatcgaggt atccggtttt gagcaaaaca
    75781 ccactcacag caaaggccat agacgccgca caaccaaagg acaagcaata caaactcacc
    75841 gcttcactga cgcccggcct gtttttgctg gtgcatccca acggcagcaa atactggcga
    75901 ttccggtact ggatagacaa gaaagagcgg ttacaggcta ttggggtata tccgctgatt
    75961 agcctcaagg aagcccgcaa acgcgccaca gagagccgtt tactgatagc ccagggaatt
    76021 gacccaatgg atttggcacg caaggagaaa gccattgatg cactcaacat ggcggcaggt
    76081 tttaagacgg tagcggagga ctggctggct accagggtag gcggctggag tgagagctat
    76141 acaaagcagg tcaaatcagc actggagaaa gacgtttatc cggtacttgg caagcgttca
    76201 atcgttgata taaccgcccg tgacgtgctg gcattactcc agaagaaaga acgtaccgca
    76261 ccggagcagg cccgcaagct acgccagcgc atcggtgaga tcttcaagtt tgccgttatc
    76321 accgaactgg taacgaggaa tccggttgca gatcttgata cggcactgaa ggctcgccga
    76381 cctggtcaca acgcgtggat accgattagt gaaattccgg ccttctacaa agcccttgag
    76441 agggccggga gcgtccagat tcaggcggca atacgtttgc ttatattaac ggcattaagg
    76501 accgcagaac tgcgtttgtg ccgttgggaa tgggtaaacc tggaggacgc aacaatcaca
    76561 ttgcccgctg aagtcatgaa ggcccgccgc ccgcatgtgg tcccgctatc acggcaggcg
    76621 gtcgagctat tgcaggacca gttcacacgc agcggataca gtgttttcgt gttcccgggc
    76681 cgattcatgg ataagccatt atcagccagc gcgatactca aggcgcttga gcgtatcgga
    76741 tacaagtcaa tagccacagg ccacggctgg cgcacaacgt tcagcaccgc acttaacgaa
    76801 agcggcaggt ataaccctga ttggattgag atccaactag cccacgtccc gaagggaatt
    76861 aggggagttt acaaccaagc ggcctatctc aaaatgcgcc gtcaaatgct tcaggactac
    76921 gccgacgcga ttgattcgat actggctgat ggtatcaatc cgcttgaact gtaatgaatg
    76981 attaatcacg ttttccgacg actccaccgg agtaggccgg ataaacactg gacaccttca
    77041 aaataactat tcacttttgc cggattcgtc cggctttttt tgtctttgcc gatgggatca
    77101 attattccaa ttattccaat tattccaatt attccaatta ttccaattat tccagtattg
    77161 ttggaataaa taaatatcaa tgacttaaag ccgtgtttgc aacagggtgg atcggggatc
    77221 gaaaatagga tcaacacaaa aacaccctgg aggctacagc acactagggg gacacaaaaa
    77281 caacacagga aaaaatacca tgtaccaatc agcaaccgcc tacgtggcga aaccaggcga
    77341 aacactggtc aaacacaaaa ttgcaacact ccaagcactt caaacactcc aagcccttcc
    77401 ggaacaggtt caggccgcct ttcgtgatgg catgaacatc atccgcttcg atgggtatcg
    77461 ctgggaatac aagccagcaa ctggattcgt gcgcatagat caaatcttgt cgccagccaa
    77521 tgaagagcca gcgctagcca atgcagaacc agcgcaggag caggagcagg agcaggaaca
    77581 ggaacaggaa cagaataaag cggcggctat tgccgagttc aaggagctgc aaagacaaca
    77641 aatcacagcg cagcaagaag aatgccgcca cccgctggca atgcccaagc cagtagcgaa
    77701 agccaacaca gacaagccga cttcatggtt taacgaagaa gcggaagaag atttgcgttt
    77761 tgatagtaaa gaggagcagt tgcctgaaga gcgggaacag gtggaaacgg aagaccagca
    77821 aaacgattat cgcaaccttc cagccgtcaa taacgctatg gcttacctct tcaaaagcgg
    77881 gctgtttcac aagctgaaag gcgatgagtt aaagctatat acgtggatgg tatccaagtg
    77941 caggtatcgc ggtaacgaca aatatgagat ttggtattcc cgcaccaaaa tggcaaaagc
    78001 gttaggtaag agcgttgaca cgatagatcg ccacctggaa gcgttgagaa agctgggact
    78061 tatccaggaa atgcgccagg cactgagggg caaatgtgga aaaacctaca gactgcgaat
    78121 cccgtcaaaa ggcacagcag aagaggcctc aatgacggca cagcagcacg aaagagaagc
    78181 aaaagtcagc tacctggtac actaggcagc gggtcagcaa aggggggcaa atgccccttt
    78241 tttcatgtca gataatcaga tcattacctc aataaactaa taatcacatc gcggttacaa
    78301 ccagtttgat agtgatagct caaacccaga cgtggcgctg gctgcaagca aaataagccg
    78361 caaaaatgcg gtcctatttg ccagaacctt ttattattca attggttagt gcaagaaaaa
    78421 gcgtgattac tgttcaagaa gtatccagaa aggtcatgat tttgccatga ttttgacgct
    78481 gaaaaaaaat aggtgggcat ttttgcccac tcactagccg caaatttgcc cactcactag
    78541 ccgcattttt gcggcccaaa gtaataaata cagagaaata ataaatacag atcagcgcca
    78601 agatttttcg tcttttcggc ggaatcaaga gactagtgct tacgtcctga taacctccag
    78661 agcagacctt aacttctgtg cagcaggact acgctcattg ccgctcggct aggtcaaatc
    78721 ctcacgataa agctgttcgg attgtgacca taatgttaaa ggactcttta aatcattcct
    78781 tgacgaatta actagctggc ttaagcggaa tagcatgcag gataaaaaca aaaccagctt
    78841 agcagtgggg agagcttcgc ttcgctcgtc tcctggggcc aatgctcaat gaaacgacgt
    78901 aaggcacgga gcgctcacca taggtagaca cataaacatc tgagagcgca ccttctatcg
    78961 cgctacgcgc tcaataaaaa ccaacgacac cgccacactg caagaccaat ggtttttatc
    79021 gcgtatacag accagacagg aaacagcagc aaagcagcgt atagccaggt gcagacagca
    79081 cactcacacg acgcacagca ggcttaagtg atcactcagg aaactttatc cccgccgccg
    79141 atcaccgcga actatcatga ccgcgccaaa ttcgaaagga atggcccata gaacgcgatg
    79201 aacacacata agcgccaact tgcttacctg aaaacttaac gtcgccacag agcgattgag
    79261 agcgcttaaa atcgatgcga tttcattaaa tttttctcac tcgcctgaat ggtggcgatc
    79321 aggactgatc gcaacattgc cccgcctcga tattgagcgc ataatataca catacattaa
    79381 gcggaaaaga gataagttta ttgtgaaaat cagggggtta cacatcatgc gcatatcaga
    79441 acaacaacgg cagatcctcg actatctggc aaaaaacgaa tcaatcaccg cacgacaagc
    79501 cgcagatctc gtttatggtg gcaatgttac ccgcgtccag gttgacgccg cccgccgctc
    79561 actcatgacg atggttagta atgggctaat caggaaacaa ggccgcgctt tcgtagctga
    79621 aagacatatc aatcacgctt atagccagga cgttgaagaa ttgcgccgcc agatcctttg
    79681 ggtattcagc aaacagggtc aggagtattt cctttctgaa cacccaaatt tcgccgccaa
    79741 agagttaaca gtaagcggat tgtcgtgcgc actggattat cttgtcggtg aaggttgcgt
    79801 tacttacaag cagctatggc acgccctgaa tacgcttgta gaagatggtt tactcattgc
    79861 cacgcgtgag aacatcgaat actggataat gccgcgttca ggtcgccgca atcatgcacg
    79921 gtatatttca ggcaacagat acagaacgcc ggactttaat ccgacacagg aagatcggga
    79981 ccgtgccgaa atgatgtgca gggaatagga gaaaagaatt atggataagt tattttcgat
    80041 gcagtgaaca caagcgggcg caatgcccgt ttttattgcc ttattccacg cctgacgaat
    80101 caatttgatc acacctgagc aaatgcatag ccagaagtta aggccgttat attgcgattg
    80161 ggagcgtttt aaacggtatc aatttaatta attctgatgt ggtgatttgc aggcaaaaaa
    80221 aacccgccgg agaagcggga aatagagaca tatcgttgta gttattaaca gatcactttg
    80281 aggtcgaggg cggctaaccc tcgctaatat tatcccgcca ttaccttgcg ggtgcaaata
    80341 atcttttatt gctttgatga tttaataaat agcacttttt gctaacaaaa accgctgtca
    80401 atatatggcc aaaaatgtca acagaaaaaa taaggtttat aatattaagt atcacatggg
    80461 taatgggttc agggaatcgg aatccaccgc ctcacggaaa ttcaaaattt gcttcttctt
    80521 tcctccgtgg ggcggcttct ttttcgtatc gaggtattta tgaaagcaat aacattagaa
    80581 cagttcgcag caatggcgaa tgtatcgacc agaacgctga ggcgctggct aaaaaaacca
    80641 tcaaccggag tccctttgcc atttacccca cgcggtactc gccaattatt ctgggagcac
    80701 gaatgcaaag aatggttgta cagaacagaa cgataataat cacgcatcac attttgaggt
    80761 aaacatcatg accaatatcg aaagattaga acgctgtgcc gatattatgc ggcgcagatg
    80821 gatttatgac cctgaagcgg gtttattgtt tagccgtgaa acaaaagacg ttatcaaagg
    80881 cagcatgacc aataaagggc atttggttgt aacggtacat aaaggtaaat tcaaagtgac
    80941 actcacctat cagaaagcct gctatatata cacatacggc gcttatgacg agacattata
    81001 tgaagtgcac cacgttaatt tggacaaaca ggataaccgc atcaagaata ttcgcctgat
    81061 gccgaaagaa aagcaccgcc ggatacataa aaacgtccgc aaacttattc ggctgggcca
    81121 cacacaatgg cttcaaccgc aattaattga acaatcacta taaacacagt agtaaatacc
    81181 atgcattcat tagacagtta ttttcagcgc acgacagccc ctaaatcagc cgcccaggaa
    81241 cgccgtgagg aattccatga gaaagtgatg cgttctgctg attatattgc ggacaagttc
    81301 gtggaaacgg ttcgtccact tgttgatgaa gtagctgata aacttcagtc tgaaatgccg
    81361 gaagatatgg aaggcactgc aaagcggcga ctcatttgcg aattaagtcg tcgttttggc
    81421 gtgtcaataa gcgcttttaa ataatcgaaa aaagggggga aagataatgg acgcatttga
    81481 ggtactggca aataaaggcg ctgaactggt agcacagcgt gataaaaccg ccaacgaagg
    81541 ggaacgcgca ctgctaaata aacagattaa agcgatccgt atggcccagt ttaaattaat
    81601 cagtaatgaa gttatcgaaa caattaaacc gcagatccca caaatcgttg ctgacgctat
    81661 taaagccgcc ggactggaga aacgcattgc cgggctaaaa acgaacgcag agaaaatgga
    81721 gatgcacaaa cagagcggtg ctccgaatcc cgatgagtac ttctcaccgg atgttgagtt
    81781 tatggcgcag gttgaggaac gtctgggtag ctgtctcacc gaggaacagc gtcgctattt
    81841 tgatggagtg gatagttcgg caggtattga cctgaactcg tatttcggtc gtgaaattga
    81901 gcattttgat gccgccccgt ctatgccgga gccagaacca gaaatggcca ccgatcctga
    81961 agctgagcaa cggaaacgcg tattcaatgc gatagggatt cggtactgat cggataaaag
    82021 aaagccggga attaccccgg ctgacttaac aatgtgacca atgaacaaat cggacaagac
    82081 aaacaggata agcagtctcg aaacaatgtt cgggaactat attagcacaa gtccggagaa
    82141 aatcatcatg ttggcactac gcatcatgca gggcatcgct aagaccctcg cagaacatgt
    82201 cttagacctg aaacattcgc cactgagcaa acaggccatg aagcgctaaa cgcttcgtct
    82261 gtgggctgaa tattcgcttg gaactatcaa taaaatcatc gacatgaaat cggggccgag
    82321 caaccagagc gcggaggaaa tggagttcat caggcgttta attctcattc gcagagacat
    82381 tcatagccag ttacacagcg tgggaatcga tattaatgat gggacgggcg attaattttc
    82441 ggacaagtac tatgcgcgcg cgaggagctg aacatgaagc aagatattgc agacaggctg
    82501 gagatactcg aaggccagcg ggcagaagcg aaacaactac gtaagcaggc cagaagagcg
    82561 cacaggaaca acgaagcaga gttactaacc aaatacatca gtttcaccaa ttactgcatc
    82621 tacgagtgtt acaaggaaga tgcagaggac tggctggatt cattgccaga acagtactga
    82681 gcagaataac accacgtcat gttgttctga ggtgagcata tcacctctgt tcaggtggcc
    82741 aaatttagtg tgccacttca gcatcaccgt tgaaatcaca ataacgattt ttttgtaaat
    82801 atgctctcac atatgtagga atagaccagg ttggcattga aacacatcta ccatgaccat
    82861 aataaagtaa ttttttgata tgtttatggt tttatattaa ttaaaatata tctaaaattg
    82921 atagttttaa aattgcaaaa tccagattgt gtttgtaatg tttaatctga ttgtattgag
    82981 tggcgtttat tatgaaaaat aaatcaggta attcttgtta tagagaagca gtcgatattc
    83041 atagctcact tgagctttca tttatattgc ataaatatca tttgcgtgta gatgagaaga
    83101 aatctcgaaa tgtattcagc gccatacgtg atgagctcat ccttgatggg gattcacgac
    83161 aaaacttagc tactttctgt caaacatggc ttgatgatga aatccgtgag ttgcttaatt
    83221 tagctattga taaaaacatg atttataaag atgaatatcc acaaatggcg gaacctgaag
    83281 gtcgctgtgt gcgtatgctt tccgattcgt ggagtttccc cgattcgcga cacaccttag
    83341 gttgctcaac cattggttca tctgaagctg ctatgctcgg ccgcttggcg ttaaaatggc
    83401 agtggtgtaa aaaacgcgaa gtacaaggta aatcaaccga acctgatttg cgatcctgtg
    83461 caaatatgct ggcataaatt ctgccgttac tgtgatgtga aattgtgtga aattccactt
    83521 gatgatcatc acctgataat gaatgctaaa gaggttttga agcatgtcga tgaaaacact
    83581 attggtgtcg ttacgacttt aggtattact tatttttgta aagcagattg cgttagtgtt
    83641 tttaagataa gtgttaaagt taatattagt ttttatattt acatcgtaat tatatgtttc
    83701 tattttaaaa atattcgaga gtataaaaat gtctaatgct aatatcgcac ccaaaagtaa
    83761 gaaaatgaca gtggggacac tcgctatcat gaatataact gctgtagtaa gtctacgatt
    83821 tataccttct gaagcagaat acggcttagg tgccattttt tactatacct ttgctgcaat
    83881 tatgttttta gtacctgttg cctttgttgc ggctgagcta gctacaacat atccacaaaa
    83941 agggggggca tttcgatgga taggagaagc atttggggct cgctggggtt ttgtaggaat
    84001 gcttatgacc tggctacaag tcatccccta ctttccaaca gtactgacat ttggtgcagt
    84061 ctcggttgca tttattgatg ttgatatggg ggtggcagaa tcaattgcca gtaataaatg
    84121 gtatatttta tttttcgtac tatttgttta ctggggagct atttgtgttg cattacgtgg
    84181 cgttggtatt ttctcgcagg tatctaagtg gtgcggtatt atagggacaa ttattccagc
    84241 tattatcctt gttattcttg gtttctccta tctgtggttc agcggtaaac cagcggtgat
    84301 cccattaggt tggggtgatc ttttccctaa tatagctaac ttccagaata ttgttcttgc
    84361 cgccagcata tttcttgctt atgcagggat ggaaatgaat gcggtccatg ttaacgattt
    84421 agataatcca actaaaaaat acccaattgc aattaccatc gcttctatag gtactattgc
    84481 aatattcctg ctcagtactt taggggtcgc ctttattatt cctacagata aaattagtct
    84541 aactcaaagt ttgttggttg cctacgatat gctgttcgaa tgggctggag tgccatggct
    84601 tggctctgtg atggcattta tgctagcaat tggcgtactg ggtggggtgg ttacttggat
    84661 tgcagggcct aatacaggtg tattagcgat cgcgaaagct ggttatttac ctaagttttt
    84721 ccaaaaaacc aatcgtcatg gaatggggca ccatctaatg tttgtacaag gtataattgt
    84781 ctcagttctt tctgttactt ttgtgataat gccttcagtt caagcagctt ttcagatttt
    84841 atcacagtta acagttatgc tctatttagt tatgtatatg ctcatgttcg ctagtgctat
    84901 ctatctacga cattctcaac ctgatacaaa acgcccatat cgagcccctg ctttattttt
    84961 ctgggcaaca cttggattct tagggtcttt tctggctttt gtattaagtt ttattccgcc
    85021 cgagcaaatc ccaactggta gtccaaccag ctatgttctt tatttgttag tattagttgc
    85081 cctattttta ttaattccaa tattaattta cgccttccga aaatctgact ggaaagatcc
    85141 aaactctgat ttcgaaccat tttcttggga aaaaagaact agtaattaaa ttttccataa
    85201 gctacaaaag atgaggttaa atatgtttag tgctgaatct ccaattggtg taatagactc
    85261 aggtgtaggt ggattgacat cggtaaagga aataattaat ttgcttcctg gtgaagatat
    85321 tatctattgt ggagataata tgaatgcacc ttatggcaac cgttctgccg atgatatcat
    85381 cagtttgact aaaaaaatgc tgactttttt acagtcaagg aatgtcaaac tagttgctgt
    85441 cgcatgtaat actatatcct caactttgga atccgaaaaa tacagcggat atgctaagag
    85501 ctttccgttt cctatcttat ccatcattga acctgcagta gaagatgtga ttcgccagca
    85561 atataaaaat gttggtatta tcgctaccga gtttactatt aaaacggggt gtcacaaaga
    85621 gctaataaaa aaattgaatt caacaataaa tgtatttgga gaaccaagca aaaatcttgc
    85681 aatgttaatt gaggaaggta atcttaatgc tcctgcaatc ttgaatgaca tcaaaaaaca
    85741 tgttaaccat ttaatttccc tccatccagt aaatgaaatt attctcggct gtactcacta
    85801 tccaattgtt cagaattttt ttgaagcagt tgcaccggat ataaaattta ttaatcctgc
    85861 acatgatcag gctatctcta ttaaaaatca cttgggacag ctaaatttat taaactcctc
    85921 aaatatagga actttgcaaa taaatactag tggaagtatg gagatctaca aaacggtact
    85981 aggggaattg agcattacga aacctcatac attcagtatc aggcagtttt aaatttatcc
    86041 ggtgcaccgt aaaatcccgt ccttctgaac tgcaccccaa aagttggaca ccaactgagt
    86101 aaggtgcatt ttatggacaa acccagttac atactgggta gtgcatccaa ttagtagaac
    86161 atgtgttttt cgataaacgc tccgatcact ttttcgtgga tctcaactga gcgtgagaag
    86221 cagattgttt tacgacccaa ccgcttaatg cgggtgcgta gcgtcagatt attacgctca
    86281 atgcgttggg tgaatatttt gccggtcaga tgcttattct tcggcacctc ccggccatag
    86341 ctgccccagt catcgctggt cagcatgccg atgttgaagg gtgtaagcag tgccagtagc
    86401 tcccggcacg tttgatcggt tcggggacca aaagtgtagg ccagtacacc gcctgttttg
    86461 gtgttgtacg cgtacccgag ccaatgttgc cgggctttac tgccaacgta gctccattgc
    86521 tcatcaagct cgcagataaa cgccacatca gcatgggcaa caggcgaaga agttattcgc
    86581 tttggcgcga gttttttaaa gtccggatga cggtgttact accaattttc agtgtcctgg
    86641 cggtatcgcg aaccccggca ccattaaagg ccatttcagt tatcagctct ttcatacccg
    86701 gcttacgtgc ttgataagta taagtgaact gaaacacacg gtggcagtca cggcagcgaa
    86761 gtctgtcacg gcctttaggg ttctgaccat ggcggtaaac ctgagctgac tgacaacggg
    86821 gacaatgaat attaacgctg gtcatgagat aacctcaaaa gcccgtatta tacatcagat
    86881 tcaactaatt agaggcatca cccatccagc attataaaac atactaacaa taactaaatt
    86941 ataataatca caacttacaa tcaaataata aatgcataag attaacaatg attttttcgg
    87001 gttatattta tctaaaaaag cagaatatta tgtaacttat tattttctaa gcgaaaattt
    87061 attgtgcggt cagactaaca aacttgcgta ccacccgaag aacatgcgaa gatttaagta
    87121 ttctttcttt tttggttttt atttttcatt ttctcttttc ttctctttcc ggttctcttc
    87181 tgttctggtt ttggtttttt cctttttatt tttattcttt cttcagggtt atgctaaaac
    87241 accaaaacat ttttcaccac aaaagcatcc aatcatcttg ccagaccaat caccttaacc
    87301 ggatacactg ccggatataa tcccactaac acccagccac caagcaccat acctgataac
    87361 taccgtattc aggaaggaaa tgcgagtgat ttcaccgtca ccggcacaac cgatccgcca
    87421 aaaagaggcg taccaatgtc gatatagtcc cccgcgcgga caatcacttc gtcaatgact
    87481 gccaacggga gttgttgtag ctgtgggcgc aacaacatgc ccagagcttt accaaagtcc
    87541 tgcccggcca ccaccagcag gggatgcgga ttcggaaaac gcgcgacgaa atcgaccagc
    87601 gcgttgatga ccgtcagtac cgcggcgtaa cgcacaggca gcgaggcggg aagcgccagc
    87661 acgtacacgt cagttttggg atcaagatcc agctgaagca gcgcctgttg ccaggcactc
    87721 accagatccg tttcatcaat cgggatcgcc accggcaaat tgcgcagcgg cagttgtacg
    87781 ccctccagcc agattgtgct gccagagagc gaaagggtat gtgcacccgc gccaatcacc
    87841 gtggcgcata cggtttgcgc cggaaactgc acattcatct cacgcaggcg cggatggtca
    87901 tgcagcgccg ttgccagaag tgggccaata tcggcaaaac agaacgggtc ggcgggctgg
    87961 tggcgataac attcgcccac gcctccagaa agcgtaatga tttcgggcgt aacacctgcg
    88021 ggcagcaaac cggtttgcat caatgcctgc gcgagcggcg aaagcgttcc gtcaatcact
    88081 tcgacaatca gcgccgccat ccgccgggta acctgcacca gctgcgcgcc ggtcagcgaa
    88141 cgggcgtcag tgcctgcacc gaagcactca tccacaatca tctgccccgg tttatgagcg
    88201 taaaccacgc gcccctggct gtcggtttcc agcaggcgac caccgacgtt gagacaggca
    88261 gtgccgctga tttttccggc atcgaacagg gcgtagttcg cggtgccacc gccgatatcg
    88321 atattcagta cccgacacag ccgttgttca gaaagggttt gcgccccggc tccgtgaccg
    88381 gcgatcacgg attcgaggtg cggcccggcg ctggcgacga caaaatcgcc cagcgactgt
    88441 ttttccttca tcactttttc catcagctgt gcgaccaggc tttcggtaaa ctggccttcc
    88501 ggcagctggg caatgatggt ttcgcggatg cgctggcttt cggttttgtc ggctggcacg
    88561 ctggcgggca cggacgccgt taccgggatc gattcatcac attcggtgat ggtaaaaccc
    88621 agtagatccg acacttcacg ggcttccggg gtaataatgc tggcgcgcag aaccacggac
    88681 attgcctgtt cgccgcgcgc gtgtgcttca cgaatatcat tcgctgtgat gagttttttc
    88741 acctgcttcc tcctgtttgg tgagagatgc cagataatcc accagttgct gcacgctttg
    88801 cgggtcgtgg ctgtttagct cgaaaatcgg ctcttcgaaa cccgtctcaa gcaataactt
    88861 tcgcgttgcg gcgacgtcag catctggcat gtccgtttta ctgatgacga cgatttgccg
    88921 tttgctgacg ccaatatcca gcaacccggc aggcaaccga ctttccgggt catttgcgcc
    88981 atggacatag atcagcatgt ctacatcctg tagcgtggta attaaggcgt gataccagcg
    89041 gggatggcta aaatattcac ccggcgtatc aatatcgccc ttatcattaa attccacggc
    89101 ctgtgttttt ctggcgaggg tataattccc ctgcaacgca ttaaataacg ttgtttttcc
    89161 tgccccgacg ctacccacaa acgcaattcg tttcatgaat gcctcgtatt aacttttggt
    89221 catttcacaa agcgtataat ttaataagcg acctaaaccg ctgacggttt gtgataacgc
    89281 ttcttctact gcgcccaccg agccataaat caccaatgca ccgctgaagc ggtcgagaaa
    89341 accgatatgc acatcagcgg ctttcagcgc taaatcaccg gcgatcatcg ccgtttcgcc
    89401 gggagttagc gtcataatgc caattgcgcc cgcatccgga acgccgattt tcttcgccag
    89461 ttcctcaccg ggatgcgcga tgagatgcgc cagtgtgacc tgtttgccgg gcacaaattc
    89521 ctgaatgatg cgttctttat ccatgagtcg ccgcctgtga aaaactttcg tgtatcgtga
    89581 caatttttcg tcagagtaaa taacaaaatt cggcaagcgc gtttaaaagg tgaggtagat
    89641 cactaagaat ggagagatat ttactgttat gaataaactt tcatatgcga cgcatgaata
    89701 taaataaaat aatatcgaga tattttatat actgattttt ttattattcg caaactcagt
    89761 aataagcttt gctaatatat ttcaggaaaa ccgcttaccc tgaatattca gggtaagcgt
    89821 gagagttaag aatgaattac agcggttggg tttgcgcttc aaccacggcc agcgccacca
    89881 tattgacgat acgacgcacc gatgcgatcg gcgttaacac gtgaaccggt ttcgccacgc
    89941 ccatcagcac cggaccgaca gtcacacctt ccgagctgga aacgcgcagt aagttgtaac
    90001 taatgcgggc agcttccatg ttcggcatca ccagaatatt ggcagaacct ttcaaagggc
    90061 tgtccggcat acggtcgttg cgaatcgctt ccaccagcgc tgcatcgccg tgcatttcac
    90121 catcaatcat cagatctggc gcacgatccc tgaccagttc cagcgcctga cgcattttgc
    90181 tcgacgacgg gcagtcagaa gaaccaaagt tggagtgcga caacagagca acgcgcggct
    90241 caataccaaa acgacggaca gtttctgccg ccatcaaggt gatctccgcc agctcttctg
    90301 cgtctggttc gtcattgacg taggtatcgg caacaaaggt gttaccactc ggcagcagca
    90361 gcgcgttcat tgcacctgcg gtgtgaacgc catcgcgata accaaagaca tttttcacca
    90421 cgctaaaatg ttcatgataa tcgcccaccg taccgcagat cattgcatcg gcttccccac
    90481 gctgaaccat gatcgcgccg atcactgtcg ggttactgat cagcgcacgc tgcgcctgtt
    90541 cctgagtgac accgcgacgc ttcatgatct ggaagtattc ggtccagtac tctttaaagc
    90601 gcggatcgga ttcgttattg acgatttcaa aatcaacgcc cgctttgatc tgcaagccca
    90661 gtttctgaat gcgcatttcg atcacgttcg gacgaccgat aaggatcggt ttcgccagtc
    90721 ccagcgttac cagttcctga gtcgcatgca gaacgcgcgc ctcttcccct tccggcagaa
    90781 ctacgcgctt cggcgctttg cgagcctggg agaaaatcgg cttcataaac aggttggttt
    90841 tgtaaacgaa ctcagtcagc ttgtcgatgt agacgtcgaa atcagcaatc ggacgcgtcg
    90901 ccacgcccga ctccatcgcc gcttttgcga ccgcaggagc gatcttaacg atcaagcgcg
    90961 gatcaaacgg ttttggaatg atgtattccg gaccaaagct cagatcctga acgccatacg
    91021 ctgaagccac cacttcactc tgttctgcat gggccagttc tgcaatcgcg cgtaccgccg
    91081 ccagtttcat ctcttcgttg atggcggttg cgccaacgtc aagcgcgcca cggaagatga
    91141 acgggaagca aagaacgttg ttcacctggt tcgggtagtc ggaacggccg gtacagatga
    91201 tggcatccgg acgcacttct ttcgccagcg gcggcagaat ttccggttcc gggttcgcca
    91261 gcgccaggat cattggtgcc cgagccattt tcttcaccat ttcctgggtc agcactttcg
    91321 ggccggaaca gcccaggaaa atatccgcac cttcaatcac atcatcgagg gtacgtttgc
    91381 cgtcatccac caccgcatat gcggctttgg tttccgccat atttggctca cgcccctgat
    91441 agataacgcc ttttgaatcg caaaccacga tgttatgttt ttgcagcccc agcgctacca
    91501 gcaggttcat acaggcgatt gctgcggcac ccgcgccgga aaccaccatc cgcacgtcgg
    91561 agatgttttt ctccaccacg cgcaagccgt tgaggatggc ggcagtgctg atgattgccg
    91621 tgccgtgctg atcgtcgtgg aataccggaa tattcatccg ctcgcgcagt ttctgttcaa
    91681 tatagaaaca ttctggcgct ttaatgtctt cgaggttgat gccgccgaag gtcggttcga
    91741 gcgcggcgac gacttcaata aatttgtccg ggtcgagttc atcaacttca atgtcaaata
    91801 catcaatccc agcgaatttc ttaaatagaa cgcccttgcc ttccatcacc ggtttgcctg
    91861 ccagcgcgcc aatgttgcct aaccccagca ccgccgtacc gttagagatc accgcaacca
    91921 ggttaccgcg cgcggtatat ttgtaggctt ttagcgggtc tttttcgatt tcaagacaag
    91981 gtgcggcaac gcctggtgag taggccagcg ccagatcgcg ctgcgttgcc agaggcttgg
    92041 ttggagaaac ctggattttc cctggaactg gaaattcatg gaaatcaagt gcactttgtt
    92101 ttaactggtc atccatttgg ttgttccttt cacgtaacgt tcacaaataa agtgtgtggg
    92161 caacagcccc tgcccacaac gtggcgcaca ttattaccct gccggagtct acagactttg
    92221 agcaagtcca aactctcacc attaatataa tgttttggta acaatcctat aacactgatg
    92281 ttacctgctt aatccagcaa taccatgcct gtctgctatg cttttttgat gcgtttagcg
    92341 aaatttctca gaagtgtgaa ttaacgcact catctaacac tttacttttc aaggagtatt
    92401 tcctatgaac gagttagacg gcatcaaaca gttcaccact gtcgtggcag acagcggcga
    92461 tattgagtcc attcgccatt atcatcccct ggatgccacc accaatcctt cgctgttact
    92521 caaggctgcc ggattatcac aatatgagca tttaatagac gatgctatcg cctggggtaa
    92581 aaaaaatggc aagacccagg aacaacaggt ggtcgcagcg tgtgacaaac tggcggtcaa
    92641 tttcggtgct gaaattctca aaatcgtacc cggttgcgtg tcaacagaag ttgatgcacg
    92701 cctctctttt gataaagaaa agagtattga gaaggcgcgc catctggtgg acttgtatca
    92761 gcaacaaggc gttgagaaat cacgcatttt gatcaaactg gcttcgacct gggaaggaat
    92821 tcgcgcggcc gaagagctgg aaaaagaagg tattaactgc aacctgacgc tgctgttttc
    92881 ttttgcccag gcgcgggcct gtgcggaagc aggcgttttt ctgatttcgc cgtttgtagg
    92941 gcgtatttat gactggtatc aggcacgcaa gccgatggac ccgtatgtgg tggaagaaga
    93001 tccgggcgtt aaatcggtgc gcaatatcta cgactactat aagcaacacc actatgaaac
    93061 cattgtgatg ggcgcgagct tccgtcgcac cgaacaaatc ctcgccttaa ccggctgcga
    93121 tcgactgact atcgcaccga atttgctgaa ggagctgcag gaaaaagttt ctccagtggt
    93181 acgtaaatta atcccgcctt ctcagacgtt cccacgccca gcacccatga gcgaagcgga
    93241 gttccgttgg gagcacaatc aggatgcgat ggcggtagaa aaactgtctg aaggcattcg
    93301 tctgttcgct gttgatcaac gcaaactgga agatcttctt gccgccaaac tataaaccag
    93361 ccacggagtg ttatatgtcc cgaaaagacc ttgccaatgc gattcgcgca ctcagtatgg
    93421 atgcggtaca aaaagccaat tctggtcatc ccggcgcgcc gatgggcatg gctgatattg
    93481 ccgaagtgct gtggaacgat tttcttaaac ataatcctac cgacccaacc tggtatgatc
    93541 gcgaccgctt tattctttcc aacggtcacg cgtcgatgct gctctacagt ttgctacatc
    93601 tgaccggtta cgacctgccg ctggaagaac tgaagaactt ccgtcagttg cattcgaaaa
    93661 ccccaggcca cccggagatt ggctatacgc caggcgttga aaccaccacc ggcccgcttg
    93721 gacaaggttt ggcgaacgcc gtcgggctgg cgatagcaga gcgtacactg gcggcgcagt
    93781 ttaaccagcc agaccatgag atcgtcgatc acttcaccta tgtgtttatg ggcgacggct
    93841 gcctgatgga aggtatttcc cacgaagtct gttcgctggc aggcacgctg ggactaggca
    93901 agctgattgg tttttacgat cacaacggta tttccatcga cggtgaaaca gaaggctggt
    93961 ttaccgacga tacggcaaaa cgttttgaag cctatcactg gcatgtgatc catgaaatcg
    94021 acggtcacga tccgcaggcg gtgaaggaag cgatccttga agcgcaaagc gtgaaagata
    94081 agccgtcgct gattatctgc cgtacggtga ttggctttgg ttcgccgaat aaagcaggta
    94141 aggaagaggc gcacggcgca ccactggggg aagaagaagt ggcgctggca cggcaaaaac
    94201 tgggctggca ccatccgcca tttgagatcc ctaaagagat ttatcacgcc tgggatgccc
    94261 gtgaaaaagg cgaaaaagcg cagcagagct ggaatgagaa gtttgccgcc tataaaaagg
    94321 ctcatccgca actggcagaa gagtttaccc gacggatgag cggtggttta ccgaaggact
    94381 gggagaaaac gactcagaaa tatatcaatg agttacaggc aaatccggcg aaaatcgcta
    94441 cccgtaaggc ttcgcaaaat acgcttaacg cttacgggcc gatgctgcct gagttgctcg
    94501 gcggttcggc ggatctggct cccagcaacc tgaccatctg gaaaggttct gtttcgctga
    94561 aggaagatcc ggcgggcaac tacattcact acggggtgcg tgaatttggc atgaccgcta
    94621 tcgccaacgg catcgcgcac cacggcggct ttgtgccgta taccgcgacg tgcctgatgt
    94681 ttgttgaata cgcccgtaac gccgcgcgga tggcggcact gatgaaagcg cggcagatta
    94741 tggtttatac ccacgactca attggcctgg gcgaagatgg tccgacgcac caggctgttg
    94801 agcaactggc cagcctgcgc ttaacgccaa atttcagcac ctggcgaccg tgcgatcagg
    94861 tggaagcggc ggtgggctgg aagctggcgg ttgagcgcca caacggaccg acggcactga
    94921 tcctctcaag gcagaatctg gcccaggtgg aacgtacgcc ggatcaggtt aaagagattg
    94981 ctcgtggcgg ttatgtgctg aaagacagcg gcggtaagcc agatattatt ctgattgcca
    95041 ccggttcaga gatggaaatt accctgcaag cggcagagaa attagcagga gaaggtcgca
    95101 atgtacgcgt agtttccctg ccctcgaccg atattttcga cgcccaggat gaggaatatc
    95161 gggagtcggt gttgccttct aacgttgcgg ctcgcgtggc ggtggaagca ggtattgccg
    95221 attactggta caagtatgtt ggtctgaaag gggcaattgt cgggatgacg ggttacgggg
    95281 aatctgctcc ggcggataag ctgttcccgt tctttggctt taccgccgag aatattgtgg
    95341 caaaagcgca taaggtgctg ggagtgaaag gtgcctgatg gtgatcgccg gatgctgtat
    95401 gttcccctca ccctaaccct ctccccagaa gggcgagggg acggtccgtg cgctttttag
    95461 ggccgcatct gcgattttgt ttaacgggtg atccacaacg tgggcctggc gtctggccca
    95521 tgccagttat cgcaggtagg ttctgcggcg taacgcacca ggcgaaaacg ctgaccgtca
    95581 aagcgccagc gcgcctgaat gccacaatcg cttaatccgc gccctttcgc taaggtcacc
    95641 agttcacgcg atttctcatc aaatgttgcg ttcatcagtt ccagttcatt cgtctcctga
    95701 ccgttgttga acggcaaacg caaccgaacc gggcgcgagg ctagcggctt tttacgcgac
    95761 acaatccatg ccaaatcaat ggtgttatag gcccctgcct cacagctaat catcatcagt
    95821 gctttatcat cggtcagcgc agtgacattc acctcacgac gtaacggatc aagcgagcag
    95881 cgcagaccat tcatccgcca gtttccataa tccagcaaat cgttacgttc ttcgagtgag
    95941 agtggcgtcg gcgttgggtt aaccaccgcg acctctttca gcgcaggcgc gggcggtacg
    96001 ctgagaggcg gttcgtcccc tttcttgatc cacgcggttt cactgccaac gcgcttttgc
    96061 tgagcatcaa taaacaacaa cgccgctttt aagccgctca gagaaatggt ctgattgccg
    96121 tcgcgtaagg tgatcgcctt cccttcctga attatctgca aaaacgcggt gatggttgcc
    96181 gtatcatcgg ttactaataa ccatggtgaa atccgccact tgtcaccact taatgccaac
    96241 ggctcaccat ctaatagcag ccgtggcgct atctcccctt cagacgcatc cggcgacttc
    96301 aacccaccgc gctcaatacg taaaacggca tcggtatgcg ccccagcgct gcggctcagg
    96361 gtcatcacca gtccattatg atcgcccgta ttgcgcgcca cacaaaaatt ttgattatta
    96421 caggtgacct gccagtcgga aaacgcccgt tgcgctggtg ccgcccacac caaagacgtc
    96481 ggcaacaaag cgaaaaaaag agaaggaaaa tgcgatagcg catggacggt acgaccccag
    96541 aagttcaaac aaacagcaca agtcgtatct tcgttgcgca ggtagcgcgg ctcaatcgga
    96601 tttgtcggat tgagccaata ataaacaacg aaatattatt gctatttttc agtccattaa
    96661 atgtgacatt tgcaaatagt taagcaataa caccgtctta ccgtcacgta tctcgccggt
    96721 tttgatcatc tccagcgcct ggctgaacgg cagctcgagc acttcaatat cttcatcttc
    96781 aacaccgcca ccggcgttag cgcgctgatt gtcactgtat tcggcgataa aaaagtggat
    96841 tagctcagtc acaccgccag gcgacatata cagttcaaat aatttgcgca cttcgcccac
    96901 ttcatagccc gtctcttcaa tcgcttcttt acgaatgcac acttccggtt cgtcgttatc
    96961 cagcagcccg gcgcaggttt caatcagctg cccgctttca ttgccattaa cccaggtagc
    97021 gacacggaac tgacgaatca gaaccacgct ctttttcttc gcgttgtaca ggaggatcgt
    97081 cgcaccatta ccgcgatcgt aaacttcacg tttatggcgg ataacttcac catctttgcg
    97141 cgtgagatcg taagtaatgt tgtgcagggt gaaatagtta tcggagagaa ttttgtcttt
    97201 aatgagggtg atttgttgcg tcataccgac tccacagcgc gaaatgaaca attatattac
    97261 gctgtgaagt cgggtttgtc tgcgcaggct atcagtgaga tttgactgat tttaccccca
    97321 gccagtcaat aatcccctgt gccgcatgac gtccttctgc cattgcggta accaccagat
    97381 ccgcaccacg cacggcgtcg ccaccggcga agattttcgg attggtggtc tggtaacggt
    97441 actggctttc cacatccgcg atgatgcggc cccatttgtc taccgttaca ccgtgcgact
    97501 ccagccacgg catcccgtgt ggattgaagc caaacgccat aatcaccgca tcggctggca
    97561 tgacaaattc actgccctca atcggtacag gtcgccgacg cccctgggca tccggctcgc
    97621 caagacgcgt gcgcaggaaa cgaattccgc aaacgtgacc ttgctcattc agctcaagcg
    97681 ccaccggctg gacgttaaat tcgaagttgg ccccctcttc gcgggcgttc ttcacctctt
    97741 tcttcgagcc tggcatgtta gcttcatcac gacgataagc gcaggtgacg ttactcgcgc
    97801 cgtggcgcag cgcagtacgc acacagtcca tcgcggtgtc gccgccgccc agtaccacga
    97861 cgttaagtcc ggcggtattg ataaacggct cttccggtag ctcttcgagc cccatcacct
    97921 gtttagtgtt ggcaatgagg aacggcagcg cgtcataaac gcccggcgta tcttcattgg
    97981 gtaaacccgc tttcatggaa cggtaagtgc ctacgccaac gaagaccgcg tcgtattgtt
    98041 ccaaaagcga atccaaagag acatctttac ccacttcaca attgagttcg aagtgaatcc
    98101 ccatcgcgct gaagatttcc cgacggcgtg ccagcaggga tttatccagt ttgaaagaag
    98161 gaatgccgaa agtgagcaag ccaccgattt ctggatggcg atcgtacacc gtcaccgcca
    98221 cgccattgcg ggtcagaaca tccgcacagg ccagccctgc cggacctgcc ccgataatcg
    98281 ccacccgctt gtccactttg gtgacatgac ttaagtcagg acgccaacct ttcgccaacg
    98341 cctgatctga aatatagcgt tcaatgttgc cgatagttac cgcgccgtgc tcatcgcgaa
    98401 tagtacaggc accttcacac aaacggtctt gcggacaaac gcgtccggta atttccggca
    98461 gggtgttggt ctggtgagaa agctcgacgg cggcgtcgat gtttccggct ttcaccagtt
    98521 caatccactg cggaatatgg ttatgcagcg ggcaggtcca ttcacaaacg ctgtgctcgc
    98581 cgaacttaag gcagcgcgag gcttcccgtt gtgcctggtc gcagataaat ttcatcaaaa
    98641 ccggttttgc gcgcttcaat cgccagttta tccggctcgc cacgcgcggg cgttgcctgc
    98701 atttgttcga ctttactcat taccggcatt tcttgtgcag cggtactggc atgccacggt
    98761 tgatgctcct gacgtgcggt gcgcaagcgg cgggatttcg ccatgccgga gagtgcgacg
    98821 tcagtgacca gttgcagcgc gtccgccggg caattctcga cacaggcagg accgttttcg
    98881 cgccctgcac aaaggtcgca tttatgcgcc gtggctttta cttttcctgc cgcgacgggt
    98941 gtcaggacga tttgcatcgt accaaacgga caggccacca cgcaggattt acagccaata
    99001 cacttttgct gattgacctg aatgctgtca tcaacgtgac tgattgcacc attagggcag
    99061 ctacgggcgc agggcgcatc ttcacaatgg tgacaggtca ctgcactacg ttgctgctga
    99121 tgtttgataa ccgtaattcg gggatgaaaa tggtgttggc tcaggacatg ttgctcatca
    99181 ttgtgagcca tgacacaggc gatttcacaa gcatgacaac ccagacattg ctgactgttg
    99241 gccataataa aacgattcat aacgaccttc ttttttggtt gtaaaaacct tattctttat
    99301 atgagtgttg ttattacccg acttacaggg gaaccggcaa tgttcatgtg cccagaataa
    99361 gtaactattt cgctataaac tgtggcagat caaataatcc catcactgac taaattgcgt
    99421 ttcagcgaac tggaacatta atgatttttt gtggagaaga cgcgtgtgat tgttaaacgc
    99481 cccgtctcgg ccagtctggc ccgggccttt ttttacattg tgctgctgtc gattctttcc
    99541 acgggtatcg ctctgctaac tctggcgagc agtttgcgcg acgctgaggc tatcaatatt
    99601 gccggatcgc tgcgtatgca gagttaccgc ctgggctacg acttgcaaag tggcagtcca
    99661 caactcaatg cacatcgcca gttgtttcag caggcactgc attcaccggt attaaccaac
    99721 ctcaacgtct ggtatgtgcc agaagcagta aaaactcgct atgcgcatct gaatgccaac
    99781 tggctggaga tgaataatcg gctcagcaag ggcgatttgc cgtggtatca ggccaatatt
    99841 aataattatg ttaatcagat agacctgttc gtgctggctt tacagcacta cgctgaacgc
    99901 aaaatgctgc tggtggtggc gatttccctg gctggcggta tcggtatttt cacgctggtc
    99961 ttttttactt tgcgccgcat acgccatcag gtggttgccc cgctgaatca gctggttacc
   100021 gccagtcagc gtattgaaca cgggcagttc gactcgccgc cgctggatac cagtctgccg
   100081 aatgagcttg gtctgcttgc aaaaaccttt aaccaaatgt cgagcgagct gcataaattg
   100141 tacctttcgc tggaggcgtc agtagaagaa aagacccgcg atctccacga ggccaagcgt
   100201 cgtctggagg tgttgtatca gtgcagtgtt cccaggcgct gaacaccagc cagattgatg
   100261 tgcattgttt ccgccatatt ttgcagattg ttcgcgacaa tgaagcagct gaatatctgg
   100321 agctgaacgt tggggataac tggcggatta gcgaagggaa gccaaaccct gagcagccaa
   100381 tgcagatttt accggtgaca atgcaagaga cggtttacgg cgaactgcac tggcaaaata
   100441 gtcacgtttc atcatcagaa ccgctgctta acagtgtttc atcgatgctg ggacgcggtt
   100501 tgtactttaa tcaggcgcag aagcattttc agcagttatt gttgatggaa gaacgtgcga
   100561 ccatcgcccg cgaattgcac gactcgctgg ctcaggtgct ttcttactta cgtatccagt
   100621 tgacgttact gaagcgttcg ataccggaag ataacgccac cgcacaaagt atcatggccg
   100681 atttttccca ggcgttgaat gatgcttatc ggcagttacg cgagctgttg actactttcc
   100741 gcctgacgct gcagcaggcg gatctcccct ccgcgttgag ggaaatgctg gatacgttac
   100801 aaaatcaaac cagcgctaaa ctgaccctcg actgccgtct gccaaccctg gcactggatg
   100861 cgcaaatgca ggtgcatttg ttgcaaatta ttcgcgaagc ggggctgaat gcgatgaagc
   100921 acgccaacgc cagcgaaatc gccatcagtt gcgtcaccgc gccggacggc aatcacacgg
   100981 tttatatccg tgataacggg attggtatcg gtgaaccgaa agaacccgaa ggtcattatg
   101041 gactgaatat catgcgcgaa cgcgcggaac ggctgggcgg gacgctgact ttttcacaac
   101101 cttccggcgg cggcacgtta gtgagtatta gctttcgctc cgcagagggt gaggggaagg
   101161 ggaacacgcc ggctgaggtg taaataatta ccctcacacc ggccctctcc ctttaaaggc
   101221 gagagggaaa aacgtgccca tattgacctg taagcgagtg tgtcagcaat ctcatgataa
   101281 tttacattaa ctcctttttt tctccacgat tggctcgtac cttgccgcta cagtgaagca
   101341 agtcaagcct acaacaatac gcagaaacac gaggtcctct tttaatggcg aatttcttta
   101401 ttgatcgccc catttttgcc tgggtgctgg caatcctgtt gtgtctgaca ggtaccctgg
   101461 cgattttttc attgcccgtt gaacaatacc ccgatctcgc gccaccgaat gtgcgagtga
   101521 ccgctaacta tcccggcgca tcggcccaga cgctggaaaa caccgtgacc caggttatcg
   101581 agcaaaatat gaccggcctc gataatctga tgtatatgtc atctcagagc agtggcaccg
   101641 gtcaggcatc tgtcacttta agttttaaag caggcaccga tccggacgaa gccgtgcagc
   101701 aagtacaaaa ccagctgcaa tcagccatgc gaaagttacc gcaggcggtg caaaatcagg
   101761 gcgtgacggt gcgtaaaacc ggcgatacca acattctgac cattgccttc gtctctaccg
   101821 atggctcgat ggataaacag gatattgctg attatgttgc cagtaatatt caggacccgt
   101881 taagccgcgt gaatggcgtc ggggatatcg atgcctatgg ttcgcaatat tccatgcgta
   101941 tctggctgga cccggcgaaa ctcaacagtt tccagatgac ggctaaagat gtcactgatg
   102001 ccattgagtc acagaacgcg cagattgcgg ttgggcaact tggtggtaca ccttccgtcg
   102061 ataagcaggc gctcaacgcc accattaacg cccagtcact gctgcaaaca ccagaacagt
   102121 tccgcgatat caccttgcgg gtgaatcagg acggctcaga ggtaaggctg ggcgatgtcg
   102181 ccaccgtcga aatgggggcg gagaaatacg attatcttag ccgcttcaat ggtaagccag
   102241 cctccgggtt gggggtaaaa ctggcctccg gcgcaaacga aatggcgaca gcggagctgg
   102301 tgctcaatcg tctcgacgag ctggcgcagt atttcccgca cggactggaa tacaaggtgg
   102361 cgtatgaaac cacctcgttt gttaaagcct ccattgaaga cgtggtgaaa acgctgctgg
   102421 aagctatcgc tctggttttc ctcgttatgt atctgttcct gcaaaacttc cgcgccacgc
   102481 tgatacccac tatcgccgtg ccggtggtgt tgatgggaac cttctccgta ctttacgcct
   102541 tcggttacag cgtcaacacc ttaaccatgt tcgcgatggt gctggcgatc ggtctgctgg
   102601 tggatgacgc catcgtggtg gtggaaaacg tcgaacgtat tatgagtgag gaaggactca
   102661 ctcctcgcga agccacacgt aaatcgatgg ggcagatcca gggggcactg gttgggattg
   102721 cgatggtgct ttcggcggta tttgtaccaa tggccttctt cggcggcacc accggtgcca
   102781 tctatcgcca gttctctatt accattgttg cggcgatggt gctgtcagta ctggtagcga
   102841 tgatcctcac tccggctctg tgtgccacgc tacttaagcc actgaaaaaa ggtgagcatc
   102901 acgggcaaaa aggttttttt gcctggttta accagatgtt taaccgcaac gccgaacgct
   102961 acgaaaaagg ggtggcgaaa attctccacc gtagcctgcg ctggattgtg atttatgtcc
   103021 tgctgcttgg cggcatggtg ttcctgttcc tgcgtttgcc gacgtcgttc ttaccgctgg
   103081 aagaccgggg catgtttact acctcggtac aattgcccag cggttcaacc caacaacaga
   103141 ccctgaaagt cgttgagcaa atcgagaaat attacttcat ccatgaaaaa gacaacatca
   103201 tgtcggtgtt tgccaccgtt ggttctggcc ctgggggtaa cgggcaaaac gtggcgagaa
   103261 tgtttatccg cctgaaagac tggagcgaac gcgacagtaa gaccggcacc tcgtttgcca
   103321 ttatcgagcg tgcaacgaag gcgtttaacc aaattaaaga agctcgcgtt atcgccagca
   103381 gcccaccagc aattagcggt cttggtagtt ctgcagattt tgatatggag ttgcaggacc
   103441 acgctggagc gggtcacgat gcgctgatgg cagcacgtaa tcagttgctg gcgctggcgg
   103501 cggaaaaccc ggagctaacc cgtgtgcgcc ataacggcct cgatgacagt ccgcagttgc
   103561 agattgatat cgaccagcgt aaagctcagg cgctgggcgt tgatatcgac gatattaacg
   103621 acacactgca aaccgcctgg ggttcgagct atgtgaatga ctttatggat cgcggtcgcg
   103681 tgaagaaagt ctatgtgcag gcagctgcgc cgtatcgcat gctgccagat gacatcaatc
   103741 tctggtatgt ccgaaataaa gatggcggca tggtgccctt ctctgctttc gcgacctcac
   103801 gctgggaaac aggctcgccg cgtctggaac gctataacag ttattctgcg gttgagattg
   103861 ttggggaagc cgcaccgggg gtcagtaccg gtacggcgat ggatattatg gaatcgttag
   103921 tgaagcagct gccaaacggc tttggtctgg agtggacggc gatgtcgtat caggagcggc
   103981 tttccggcac gcaggctccg gcgctgtacg ccatttcctt gctggtggta ttcctgtgtc
   104041 tggcggcatt gtatgaaagc tggtcggtac cgttctcggt aatgctggtc gtgccgctgg
   104101 gggtaatcgg cgcgctgctg gcaacctgga tgcgcgggct ggaaaacgac gtttacttcc
   104161 aggtgggcct gttaacggtc attggtttat cggcgaaaaa cgccatcctg atcgtcgagt
   104221 ttgctaacga gatgaaccaa aaaggccacg acctgtttga agcgacgctc cacgcctgcc
   104281 gtcagcgttt acgcccgatt ctgatgacct cgctggcatt tatcttcggc gtattgccaa
   104341 tggcaaccag cacgggtgcc ggttccggtg gtcagcatgc ggtgggtact ggcgtaatgg
   104401 gcgggatgat ttcagctact attctggcta tttacttcgt gccgctgttc tttgtgctgg
   104461 tgcgccgccg cttcccgctg aagccgcgcc cggaataagc aataaaaaag gcgacatgcc
   104521 aatgtgtcgc ctttttcaac tttccgatta agaacctgct cagcgggttc ttgctgttta
   104581 tactttgtct caggaattac ttacgaagca taacttcgat aaagtctttc cagttcccca
   104641 gttcacgttc aatcataaca acctctctta taattatggg tattctacgg aaaaaatata
   104701 ccgtggtgaa gctaatttac tcgattgctg cgatgactac ctccggggga caaaccttat
   104761 gtaaatacta tggtcctgcg gtgataattt gtatgtgata cacagcaaca tttcgagata
   104821 ttcatacggc atctaatact tatttaattc tggttaaaat acagacagat aacaagatga
   104881 atattcttaa tgtttacgtt aaaaatgttt aatattattt aatagttgtt aatttgaata
   104941 cttcgataat gttatatttt ctgataatca tttgcaggca aaatgttttc acccttaaat
   105001 gagtatttat tctcataaat cgaaaaagga ttcattatgg ttacacttta cggcatcaaa
   105061 aattgtgaca ccattaaaaa ggctcgccgt tggctggaag ccaataacat cgactatcgt
   105121 tttcatgatt accgcgtcga tgggctggac agcgaattat tgaacggttt tatcaacgaa
   105181 ttaggctggg aagcgttact caacacccgt ggtacaacct ggcgtaaact ggacgaaact
   105241 acccgcaata aaatcaccga tgcggcctct gcggcggcat taatgactga aatgcctgca
   105301 attatcaaac gtccattgct ctacgcgcct ggtaagccta tgctgctggg tttcagtgat
   105361 tccagttatc agcaattttt ccatgaggtg tagtctatgt cgtgcccggt tattgagctg
   105421 acacaacagc ttattcgccg cccttccctg agtcctgatg acgcaggatg tcaggctttg
   105481 ttgattgaac gtttgcaggc gatcggtttt accgttgaac gcatggattt tgccgatacg
   105541 cagaattttt gggcatggcg tgggcaaggt gaaacgttag cctttgccgg gcataccgac
   105601 gtggtgccgc ctggcgacgc cgatcgttgg atcaatccgc cgtttgaacc caccattcgt
   105661 gacggcatgt tattcgggcg cggtgcggca gatatgaaag gctcgctggc ggcgatggtg
   105721 gtagcagcag aacgttttgt cgcacaacat cccaaccata cggggcgact ggcatttctg
   105781 atcacttctg atgaagaagc cagtgcccac aacggtacgg taaaagtcgt cgaggcgtta
   105841 atggcacgta atgagcgtct cgattactgc ctggtcggcg aaccgtcgag tatcgaagtg
   105901 gtaggtgatg tggtgaaaaa tggtcgtcgc ggatcgttaa cctgcaacct caccattcat
   105961 ggcgttcagg ggcatgttgc ctacccacat ctggctgaca atccggtaca tcgcgcagca
   106021 cctttcctta atgaattagt ggctattgag tgggatcagg gcaatgaatt cttcccggcg
   106081 accagtatgc agattgccaa tattcaggcg ggaacgggca gtaacaatgt tattccgggt
   106141 gaactgtttg tgcagtttaa cttccgcttc agcaccgaac tgactgatga gatgatcaaa
   106201 gcgcaggtac ttgccctgct tgaaaaacat caactgcgct atacggtgga ttggtggctt
   106261 tccgggcagc catttttgac cgcgcgcggt aaactggtgg atgcggtcgt taacgcggtt
   106321 gagcactata atgaaattaa accgcagcta ctgaccacag gcggaacgtc cgacgggcgc
   106381 tttattgctc gcatgggggc gcaggtggtg gaactcgggc cggtcaatgc cactattcat
   106441 aaaattaatg aatgtgtgaa cgctgccgac ctgcagctac ttgcccgtat gtatcaacgt
   106501 atcatggaac agctcgtcgc gtgatgagtg gttctgcaag aggaaataag catggactgg
   106561 ctggctaaat attggtggat tctggtgatt gtctttttgg tcggcgtcct gctgaacgta
   106621 attaaagatc tcaagcgcgt cgaccataag aaatttctcg ccaacaagcc ggagcttccc
   106681 ccgcatcgtg atttcaacga taagtgggac gatgacgacg actggccgaa aaaggatcaa
   106741 ccgaagaagt aagctgagtg ctcaaccgta ggccggataa ggcgttaacg ccgcatccgg
   106801 caatggtgaa cgatgcctga tgcgacgctc acgcgtctta tcaggccagg taatctgtct
   106861 tttacatcat ttcaatcaca tcatcatcgc caggcttacc gccacttagc gcttggtcga
   106921 aataatgttt cggaatggta taacgcaagt gatccagggc aaattgcata ctgcgattgt
   106981 cgatcgcatg gcctaaatcc tccacgatat ccagcgtcac gtcaccaccg gcactgatta
   107041 acgcttcctg cgccgcaact gcatgcgcca gatcgataac cggatcttca ccaccatgaa
   107101 tcaagtgaat tgtcgtggct gtagacgcgg tctccggcag gctcgcatag cgtccgttaa
   107161 aggcaatgac gcgcgaagca aggcccggtt cggctttaat gctctccagc gccataatcg
   107221 cgccttgtga gaaaccaatt agcgccgtgg cgttagcccc tactccgctc tgtttctgcc
   107281 agtagcgtac cgtttcaata aacgtcggca tgatggcatc cacacgcgcc tggcgattat
   107341 cttccgtaat accctgcact gaaaaccact gacgccccgc cggattaccg ctcggctccg
   107401 cgccaccgac actcaccacc agcgcatcag gaaacagcgg tgcaaaccag ctgcctattt
   107461 cccccattgc caccgggtta tccccgacac cgtggaaaag cagtaacaac tgttgtgcag
   107521 gtttatccgg gctttgaaca acaaaatggt catgtttcat ggcgaactcc ttaactgatg
   107581 tcattaattt tacgccgctg actcacaatg accatgggag aaaattgaag gagtcagtga
   107641 aaaaattgcc attgcgttat gcgatcgcgc agacgctcag tgcgaacctc atttagtgcg
   107701 aacagcgcct gcgcggcctc ttcccgctgg cgaaccagta acatcttgcg gcctgaaagt
   107761 ttaagtgtgg tacataactg cgcgtcactg gcgtttttct gtaaacgccc acgcagcgcc
   107821 ggtaatgcca gttcactagt ttgtagcaga cgcaataagc aacctaacga tgttaatagc
   107881 ggacgatgag cgaaagcaaa accggccagt tcaagccagt cgtcgtcaga aaggatggca
   107941 tcgtttagtg gatcaacagg aagcatttcg ccattccact gcgcgagagc ttgcgcatcg
   108001 cgacgtaaac ggtaatgctc acgttcagcc agctgtttac ccgcatcact catcggtaac
   108061 agcgccatcg ccgtatagca accgctgctg gcttcccgat gattacccat ccgcaccagc
   108121 acaaaaccgc agcgttgcca gaaacgccat aactccccag tgtaaccaaa actcaccgaa
   108181 agatagtcga ggtcatgcgt atattgcaaa gctccagcaa taagttgccg ccctgtgcct
   108241 tcccgctgac gagccggatg aactgctatc cggctaaccc gccttccgcg caatgtcgcc
   108301 gccagtggat tgctgccgtg cgccgccagc gactgggcca ccagattacc ccgcgggcga
   108361 cgaaaacctg cccataccgc ctgactgagt tcttgagata atccgccctc atccaccagc
   108421 cacaacgccc cggcaatctc gttttcgcca gccgcctgta aaaaatgttg ccctggtgca
   108481 tccatcatcc ggcgtaaatc cagcggcgag gtccggtagt gcgcaccaga caataactga
   108541 taaaccttta acggcgtttc tggctcgctt cgccataacg tttgttcaaa tgcggaaatg
   108601 acgatattgc cttgtggtgt atgggtgaag ttttcatcgt taaaaaccag tgcctcacta
   108661 accatttttt ccagcgggca tccctgtgcc cagcggatcg gctgttgcag ttcaaaacgg
   108721 tgtaaatgcg gaaagcgagc gcaaaatttc agcaaaaaac cacgtccggt accttcgtag
   108781 ccctgcaccg tagtggttaa caacgttcga ggaaaacgcg ataccagttg atgcaacaac
   108841 ggcgcaggta tggctgcggc ttcgtcgacc accagccagt cggcttgctc atcgctggct
   108901 aacaaggcat ccggtgcaat aaagcgaaac ttctcgcccg caaattgtgc cagtacatcc
   108961 gttgccgctt ttgcgggcgc ggtgacaatc gcactgcccg caatacgaga aatgagttgc
   109021 cctgccaacg ccgacttacc gcgcccacgc gcagccgtta ccgctgccac gcccggcggc
   109081 atggtcagta gctgctgtaa gagttgctgt tgttctggtt gtggtgcgcc ggtcgcgggg
   109141 tgccagtcag tacggggagt aaaatgcgcc aacgagaacg gctggttttg ccgccagagg
   109201 atagcgtcgt tatccgccgt aagtacgcgt ttgagatgct ggacaaaatg cggcgtcgca
   109261 atagggtcag ggcaatcact ccagcgcagc gagtcggcat caggttggtt ttcccactct
   109321 tcccatacag ggagtaacaa aaccagccag cttcccgctt tcaacgttcc gctaagtgct
   109381 gcaaaggcag cggcatcaaa gccgtggcgg gcgtcgaata ccgcatgccg gaactcgcgc
   109441 ccaagtaaag tttgtagcgc cgagggtgaa cagtggtttt cagcatctag ctgcggcgaa
   109501 atccacagcc agtcgccagg taaggcatca cgcaacttaa gagcatgatc aaaacaccaa
   109561 ccctcttctc cgctcaacac cagcaagcgg cagatccctt cacgtttcat ttgcgctgtt
   109621 aatgtgtaaa gcgcagtcag ttcagccatc cctgccccgg aaattaaatg cttttaccaa
   109681 aagtattgca ttgtgccgga tcgccgctgt cgaaaccacg tttaaaccag ctgcagcgtt
   109741 gctgagaagt gccatgtgtg aaactgtctg gtactactcg cccctgactt tgctgttgta
   109801 aacggtcatc gccgatggcc tgcgccgcat tcagcgcctc ttccagatcg ccagtttcca
   109861 gaacgccttg ctgctgcata ctatgccccc agacaccggc aaaacagtcg gcctggagtt
   109921 ccatacgcac agataagcgg tttacttccg cctgcgtcgc gttttgttgc agttgacgaa
   109981 ctttcggctc gatgcctaac agtttctgca catgatgacc gacttcatgg gcgataacgt
   110041 acccctgggc aaaatcgcca tccgcgccaa gtttgtcttt catgtcatca tagaaggaga
   110101 gatcgatata aactgtgcca tccgccgggc aatagaacgg ccccataatg gactggcccg
   110161 ccccgcagcc ggtgcgcgtc attccacggt acatgaccag cttcggttgc tgataggtct
   110221 tacccatctt ttcgaactgt tgtccccagg tgtcttccgt ggttgccaga atcaccgagg
   110281 tgaattttgc ggcttcgtct tcattagggc taattgaccg cgttgattgt tgttgagaaa
   110341 ccggctgccc ggtcatcaac ccggttaaat caacgccata gtagcctgca accagcacga
   110401 ctatcagtaa aatcagcccg cctttaccgc tcggcagacg gaaaccggga ccgcccattg
   110461 acggaccgcc agagctgttg cgcctgtctt caacattgtc actttcacgt cgcccttgcc
   110521 aacgcataga tacctcaaca atatattcat tatagaaatg atcgtaggtg gttcagagga
   110581 agattaccac aggaaagcgg gatgaaatgg cggaggaatg agcgttcaat gcatatattc
   110641 cgagagcggt tgtcttgccg tgccagctgc acggcaagat gatgaacaga aaaatcagtc
   110701 cagctgtaca cccaggcggt gggcgacggc ttcataggct tcgatcaggc caccgagact
   110761 ctggcggaaa cggtctttgt ccattttctc cagtgtttct ttgtcccaca ggcggctgcc
   110821 gtccggggag aactcatcgc ccagtaccac ttcacctttg tacagaccaa actccagctt
   110881 gaagtcgacc agaatcagac cagcatcatc gaacagtttt ttcagcacgt cgttcgcttt
   110941 gtaggtcagc tctttcatac gcgccaggtt ctctttgctc acccagccaa aggtttcgca
   111001 gtaagattcg ttgaccatcg gatcgtgcat ggcatcgttt ttcaggaaca gatcgaacag
   111061 cggcgggttc agctcaatac cttcttcgat tccaagacgt ttcaccagag agccagcagc
   111121 acggttacgc acgacacact caaccggcac catatccagc tttttcacca gacattcggt
   111181 atcggagagc agacgctcca tttgagtcgg gatacccgct tcagccagtt tgctcataat
   111241 gaagtagttg aacttgttgt tcaccatacc tttgcgatca aactgctcaa tgcgcgcgcc
   111301 atcccctgct gacgtatcat tgcggaattc gagcaccaac aggtccgggt tttccgtgct
   111361 gtatacggtt ttcgctttac cacgatacaa ctcagcttgc ttttgcatct ttatcactcc
   111421 tgggtgtgaa ttaacgtttt aaaatctttt gctgtctggt gtgccggatg ttttttcgga
   111481 tgcggcgtga acgccttatc cgaactacgt ccgacaaaca aatttcgtgc gaattaccgc
   111541 taaaatcgcg ttttcctgcc gacgcacacg tttgcgtatc atatcagaaa aaagggccgg
   111601 atgattccag ccctgtattt ttacttgcta aacgcagcct ggaagacagc taccagcgcg
   111661 tcgttctgac tctgagtcag agtatgacct ttcggatcga tgaactgtag gctgctgcgg
   111721 ttatctaaat cgccaacctg cagtttatag tcaccggatg ccaggcctgg atcgctcgcg
   111781 cccagttcct gccagtcgct gtcagacagc ggcttataag ttacggccat gttgccctgc
   111841 gaacgggtgc tgtcggtcac tttcatgccc actttttcca gcgacgctgg cagacgttgc
   111901 caaaccacat tgaacggccc gcgtacgacc agcattggta aaccggtgtc atcagctgca
   111961 ctttgtacgt ccatagtggt ggaggcacga ttttgcgcag cgttcgcggc gtcagtggca
   112021 gatttatcca gaccggcgga aataacgttc atcatctccg tgctgtaacg ctgcatggaa
   112081 gccgcgtctg caaccggttt gcccgcctgt tccaggttca gcagtttaac cgtaaccgcc
   112141 tgctgataac cctgcggctt aacagagatt tgataacgac cacgatactg ctcgtcttcg
   112201 tccagacggt tccattgtac ccaatcggtg gtcagtgtct gaccagcatc atcacgttgg
   112261 gtgatggtgt agtttttcgc ctgcagcacg ctaaccacct gcggccacag agtattgcca
   112321 cgaccatttt ccaccagcaa tgaagcggta tcgcccgtga actgggtacg cgcgccagaa
   112381 accagtgcca gcggctgggc tggtggacga atgtccagcg ccttaccgac agcaccacta
   112441 ccgttggtca ccgggattgc ataatcaccg gaggtcaccg gcaaaatcat tccagccggg
   112501 gcatgaagct ccgcaagcgg tgccgcttcc aggtaggctt catcaccact gacctgacgc
   112561 ttatagcgtg agtcagaact acaggcagcg agtaataaaa caagcgaaac acccgcaacc
   112621 tttgccaggc gcgacttttg aacagagtaa gccatcaaat ctccctaaac tttacagcaa
   112681 accggcatgc ttaagcgccg ctctgaccgt ctcacgacca ctgtcggtga ttggtgtcat
   112741 tggcaggcgc agcgtatcgg tcgccacaag acccagttcc ttacatgccc atttcaccgg
   112801 gattggattg ggttcgacaa atagtttgtt gtgtaatggc atcagacgct gattaataac
   112861 gcgtgcctcg gcaaaatgcc cttctgctgc cagtttgcac atctgggcca tatcacgcgc
   112921 tgcgacgtta gccgtaacgg aaataacccc atgaccgccc aattgcatga agtccagcgc
   112981 gctcgcatca tcgccgctca gcagaacaaa atcatctgaa accagctctt tgatctggtt
   113041 tacacgcgtt aagttccctg ttgcctcttt gattccgata atatttttta ctttcgccag
   113101 acggcccacc gtttccggga gcagatcgca gccagtacgg gacggcacat tatacagaat
   113161 ttgcggcagg tcagtatgct cagcgatggc tttgaaatgc tgatacaaac cttcttgcga
   113221 cggacgattg tagtaagggg ttaccgtcag gcagccgacg ataccactgt cattgaagcg
   113281 ctgcgtcagg ctaatggctt ccgcagtagc gttagcgccg gtcccggcaa ttaccggaat
   113341 gcgcccatca gccagatcca gcgtcatcat caccacatca gcatgttcgt catgatttaa
   113401 ggtagcggac tcgccagtgg tgccaacaga aacgatcgcc gaagtaccgc tggcgacatg
   113461 ataatcaatc agttttttca agctagcccg acagacatta cctttttcat ccatcggagt
   113521 aacaatcgcg acaatacttc ccgtgaacat gggccatcct ctgtgcaaac aagtgtctca
   113581 atggtacgtt tggtatggca ttaaaagcaa gcagacagaa ccgttctgat tgttgtatgc
   113641 atgatttttt tatgctttcc ttaagaacaa ctcacccctt aaaggaataa ccagtttgac
   113701 actgtcatcg caacattatc tggtgatcac tgcgttgggt gccgatcgcc ctggaattgt
   113761 gaacaccatc acccgtcatg tcagtagttg cggctgtaat attgaagaca gtcgcctggc
   113821 gatgctggga gaagagttca cgtttatcat gctgctttcc ggttcatgga atgccatcac
   113881 tctgattgaa tcaacgttac cgttgaaagg tgccgaactg gatcttttaa tcgtgatgaa
   113941 gcgcacgacg gcgcgtccgc gtccgccaat gccagcatct gtctgggttc aggtcgatgt
   114001 tgcagactcc ccgcatttaa ttgaacgctt cacagcactt ttcgacgcgc atcatatgaa
   114061 cattgcggag ctggtgtcgc gcacacaacc tgctgaaaat gaacgggctg cgcagttgca
   114121 tattcagata accgcccaca gccccgcatc tgcggacgca gcaaatattg agcaagcgtt
   114181 caaagcccta tgtacagaac tcaatgcaca aggcagtatt aacgtcgtca attattccca
   114241 acatgatgaa caggatggag ttaagtaatg aatccactga aagccggtga tatcgcaccg
   114301 aaatttagct tgccggatca agacggagaa caagttaatt tgaccgactt ccagggacag
   114361 cgtgttctgg tttatttcta cccgaaagcc atgacccccg gctgtaccgt acaggcctgc
   114421 ggcttacgcg ataacatgga tgagttgaaa aaagcgggcg ttgatgtgct gggtatcagc
   114481 accgataaac ccgaaaaact ctcccgtttt gcggaaaaag agctgcttaa ctttacgctc
   114541 ctgtctgatg aggaccacca ggtgtgcgaa caattcggcg tctggggtga aaagtccttc
   114601 atgggcaaaa cctacgatgg tattcatcgc atcagcttcc tgattgacgc tgatggcaaa
   114661 atcgaacatg tctttgacga cttcaaaacc agcaatcacc acgacgttgt gctgaactgg
   114721 ctgaaagaac acgcctgatt actttgctcc attccgtgct ggctgcgctt gcggccagca
   114781 tacctcactt ctcgtgatca agatcacatt ctcgctttcc cctgcgacac gggtgtcgaa
   114841 tccatttttt gctgaacgtt aatgaccatc atttttgtac cgttcagaat ccagttaata
   114901 cataacttat tgaatagatt gagttaatca gaatggcacc ctttatgcaa tatgaaatgc
   114961 aatgtttcat atcattttca aggagccgac atgaaccgct ttgtggtggc cgaaccactg
   115021 tggtgtacag gatgtaatac ctgtctcgct gcctgttcgg acgtgcataa aacgcaaggt
   115081 ttacagcaac acccgcgcct ggccctggcg aagacgtcaa caatcactgc ccctgtcgtg
   115141 tgtcatcact gtgaggaagc cccttgcctg caggtctgcc cggtcaatgc catctctcag
   115201 agggatgatg cgatccaact caacgaaagc ctctgtattg gctgcaagct ttgcgccgtg
   115261 gtctgcccat ttggcgcaat cagcgcttca ggaagccgtc cggtgaatgc ccatgcgcaa
   115321 tatgtttttc aggctgaagg ctcactcaaa gacggcgaag aaaacgtgcc gccccaacat
   115381 gcgttgctgc gctgggaacc gggtgtccag accgtcgcgg tgaaatgcga cctgtgtgat
   115441 ttcttgccag aaggtccggc ctgcgttcgc gcttgcccga atcaggcgtt acggctgatc
   115501 accgacgata gcctgcaacg tcagatgaaa gaaaaacagc gccttgccgc aagctggttt
   115561 gccaatggcg gggaggatcc cctttccctc actcaggagc aacactaatg gatgccctgc
   115621 aattattaac ctggtcgctg atcctctatc tgtttgccag tctggcttcg ctgtttttac
   115681 tcggtctgga cagactggct attaagcttt ccggcatcac atcgctggtg ggcggcgtga
   115741 ttggcatcat cagcggaatt acgcaattac atgctggtgt aactttagtc gcccattttg
   115801 ccaccccttt tgactttgcc gatttaaccc tgcgaatgga tagcctctcg gcatttatgg
   115861 tgctggttat ctccttgctg gtggtggttt gttcgctcta ttcattgact tatatgcgcg
   115921 aatacgaggg caaaggcgcg gcggcgatgg gcttctttat gaatcttttc atcgcatcga
   115981 tggttgccct gctggtgatg gacaacgctt tttggttcat cgtgctgttt gaaatgatgt
   116041 cgctgtcttc ctggtttctg gtcattgcca ggcaggataa aacgtcgatc aacgctggca
   116101 tgctctactt ttttatcgcc cacgccggat cggtgctgat tatgatcgcc ttcttgctga
   116161 tggggcgcga aagcggcagc ctcgattttg ccagtttcgc acgctttcac tttctccggg
   116221 gctggcgtcg gcggtgtttc tgctggcctt tttcggtttt ggcgcgaaag ccgggatgat
   116281 gccgttgcac agctggttgc cgcgcgctca ccctgccgca ccatcgcacg cttcggcgtt
   116341 gatgtctggc gtaatggtca aaatcggtat tttcggcatc ctgaaagtag cgatggatct
   116401 gctggcgcaa acgggtttgc ctctgtggtg gggcattctg gtgatggcga tcggcgcaat
   116461 ctccgcgctc ctgggcgtgc tatatgcgct ggcggaacag gatatcaaac ggctgctggc
   116521 ctggagtacc gtcgaaaacg tcggcattat tttgctggca gtcggtgtgg cgatggacgg
   116581 tctgtcactg cacgacccgc tgctcaccgt ggttggactg ctcggcgcac tgtttcatct
   116641 gctcaaccat gcgctgttca aagggctgct atttctcggc gcggacgcga ttatttcgcg
   116701 tttgcatacc cacgacatgg aaaaaatggg ggcactggcg aaacggatgc cgtggacagc
   116761 cgcagcatgc ctgattggtt gcctcgcgat atcagccatt cctccgctga atggttttat
   116821 cagcgaatgg tacacctggc agtcgctgtt ctcactaagt cgtgtggaag ccgtagcgct
   116881 acaacttgcg ggtcctattg ctatggtaat gctggcagtc actggtgggc tggcagtaat
   116941 gtgcttcgtc aaaatgtacg gtattacttt ctgtggtgcg ccgcgcagta cacacgctga
   117001 agaggcacag gaagtgccaa atacgatgat cgtcgccatg ctactgctcg cggcactctg
   117061 cgtattaatt gcgcttagtg ccagttggct ggcaccgaag ataatgcata ttgcccatgc
   117121 gtttaccaat acccctcccg tcactgtcgc cagcggaata gcacttgtac ccggcacgtt
   117181 tcatacacgg gtcactcctt cattactgtt gctgttacta ctggcgatgc ctttgctgcc
   117241 aggcctttac tggctgtggt gtcgttcgcg ccgcgcagcg tttcgtcgca caggagatgc
   117301 ctaggcatgc ggctacggct gggaaaacgc gatggctccg tcaggcaatg gcgtgatgca
   117361 gccgctgcgt gtggtcttct ctgcgttatt tcgtctacga caacagctcg accctacgct
   117421 gaggctaaat aaaggtcttg cgcacgtcac cgccagggct cagagcacag aacccttctg
   117481 ggatgagcgg gtgatccgcc ccatcgtgag cgccacccaa cggctggtca aagaaataca
   117541 gcatctgcaa agcggcgact ttcgtctcta ttgcctgtat gtggtcgccg cactggttgt
   117601 gctgctaatc gctattgccg tctaaggaaa tcaccatgag acaaactctt tgcgacggat
   117661 atctggtcat ttttgcgtta gcacaggccg tgattctgct gatgctaacc ccacttttta
   117721 cgggtatttc ccggcagata cgcgcgcgta tgcactcccg ccgcgggccg gggatctggc
   117781 aggattatcg cgatatccac aaactgttta aacgccagga agttgcgccg acatcttcag
   117841 gtctgatgtt ccgcctgatg ccgtgggtat taatcagcag catgctggtg ctggcgatgg
   117901 ccttaccact atttattacc gtttcccctt ttgcgggcgg cggcgatctg ctcaccctta
   117961 tctatcttct tgccctgttt cgttttttct ttgctctttc cgggctggat accggaagtc
   118021 cgtttgcggg agtcggtgcc agtcgcgagt tgacgctcgg cattctggtc gaaccaatgc
   118081 ttattctctc actgctggta ttggcgctga tagcaggttc cacgcatatc gagatgatca
   118141 gcaatacgct ggcgatgggc tggaactcgc cgctaaccac cgtactggcg ttactggcct
   118201 gtggttttgc ctgcttcatt gagatgggaa aaattccctt tgatgttgct gaagcagaac
   118261 aggaattaca ggaaggcccg ctgaccgaat attccggtgc cgggctggcg ctagcgaaat
   118321 gggggcaggg gctgaaacag gtcgtgatgg cagcactgtt tgtggccctg tttctgccct
   118381 ttgggcgcgc gcaagaactt tctctcgcct gcctgctgac ttcacttgtc gttacgctgc
   118441 tcaaggtttt gctgattttt gtactggcct caatcgcaga aaacacgctg gcacgcgggc
   118501 gttttttact cattcaccat gtgacctggc ttggcttcag ccttgctgcg cttgcatggg
   118561 tcttctggtt aaccggtctg taaggagcac tgacggaata tggaaaatct tgctctgacg
   118621 acgttaatgc tgccttttat cggcgcactg gtcgtttcgt tttcgccaca acgtcgggcc
   118681 gccgaatggg gggttttgtt cgccgcgctg accacgctgt gcatgttgtc gctgatctcc
   118741 gcgttttatc aggccgataa agttgccgtc acgttgacgt tggtcaacgt gggggatgtg
   118801 gcattgtttg gcctggtcat tgatcgcgtg agtacgctga ttctgtttgt ggtggtgttt
   118861 ctcggtttgc tggtcacgat ctactccacg ggttatctga cggataaaaa tcgcgaacac
   118921 ccgcataacg gcacgaatcg ttattacgca tttttgctgg tgtttatcgg cgcgatggcg
   118981 ggactggtac tctcctcgac gctgctcggt cagttgttgt tttttgaaat tacgggcggc
   119041 tgctcctggg cgttgatcag ttattaccag agcgataaag cgcagcgttc agcactaaaa
   119101 gcgttgctta tcacccatat cggttcgttg gggttgtatc ttgccgccgc cacgctgttt
   119161 ttgcagaccg gaacgtttgt gcttagcgcg atgagcgagt tacatggcga cgcacgttat
   119221 ctggtttatg gcggcatcct gtttgccgcg tgggggaaat cggcccagct accgatgcaa
   119281 gcgtggctac cggacgcaat ggaagcgcca acaccgatca gcgactatct ccacgccgca
   119341 tcgatggtga aagtgggcgt ttacattttt gcccgcgcca ttatcgacgg cggcaatatc
   119401 ccgcatgtga ttggcggcgt tggcatggtc atggcactgg tcaccattct ttatggcttt
   119461 ctgatgtatt tgccacagca ggatatgaag cggttgctgg cctggtcgac catcactcaa
   119521 cttggctgga tgttcttcgg cttgtcgctc tccatcttcg gctcgcggct ggcgctggag
   119581 ggtagcatcg cctacatcgt caaccacgcg ttcgctaaaa gcctgttttt ccttgtagca
   119641 ggtgcgctga gttacagctg cggcacgcgc ttgttgccgc gtctgcgtgg cgtattgcac
   119701 accctgccgt tgccaggcgt gggtttctgc gtggcagcgc tggcaattac cggtgtgccg
   119761 ccgttcaacg gcttcttcag taaattcccg ctgtttgccg ccggttttgc gttgtcagtg
   119821 gagtactgga tcctgctgcc cgccatgatt ctgctgatga ttgaatcggt cgccagtttc
   119881 gcctggttta ttcgctggtt tggtcgcgtc gtgccgggca aaccgagcga ggccgtcgcc
   119941 gatgccgcac cgctgccagg gtcaatgcgc ctggtgttga ttgtactgat tgtgatgtcg
   120001 ctgatttcca gcgtaatcgc cgcgacctgg ttgcagtaag gagatgatga atgaccggtt
   120061 ctatgatcgt aaataatctg gcgggactga tgatgctgac atcgctgttt gtgattagcg
   120121 tcaaaagcta tcgcctgtca tgcggatttt aggcctgcca gtcgctggtg ctggtgtcta
   120181 ttttcgccac tctctcgtgc ctgttcgccg cagagcaact gctgatctgg tccgccagcg
   120241 cctttatcac caaagtgctg ctggtaccgt taatcatgac ttacgctgca cgaaatattc
   120301 cccagaacat cccggaaaaa gcgttattcg gtccggcaat gatggcactg ctcgcggcgt
   120361 taattgtcct gctttgcgca tttgtcgttc agcccgtgaa gctaccgatg gctaccgggc
   120421 tgaaaccggc gctggcggta gcgttaggtc attttctgct tggcctgctg tgcattgtca
   120481 gccagcgcaa tatcctgcgg caaatttttg gttactgcct gatggaaaac ggctcccatc
   120541 tggtgctggc gcttcttgcc tggcgagcac cggaactggt ggaaataggt atcgctaccg
   120601 acgccatctt cgccgtcatt gtgatggtgt tactggcaag aaaaatatgg cgtacccacg
   120661 gcacgctgga cgtgaacaac ttgaccgcgc tgaagggata atgagatgag ttattctgtg
   120721 atgttcgctt tactcctgct cacgccgctg cttttttcgc tgctctgttt tgcctgccgg
   120781 aaacgggggc tctctgcgac ttgtacggtg actgtattac atagcttagg gatcacactg
   120841 ctgctgattc tggcactctg ggtggtccaa actgccgctg atgcaggaga aatattcgct
   120901 gcgggactgt ggcttcatat tgatggtctg ggcggtttgt tcctcgccat tcttggtgtg
   120961 attggctttc tcaccggtgt ttactcgatt ggctacatgc gtcatgaagt ggcacacggc
   121021 gagctttcac ccgttacact gtgcgattac tacggtttct tccatctgtt tttgttcacc
   121081 atgctgctgg ttgttaccag caataacctg attgtgatgt gggcggcgat cgaagccacc
   121141 accttaagct cggcgtttct ggtaggcatt tacggtcagc gttcatcgct ggaatcagca
   121201 tggaagtaca tcattatttg tactgttggt gtcgcttttg gtctgttcgg taccgtgctg
   121261 gtatacgcca acgccgccag cgttatgccg caggcagaaa tggcgatatt ctggagcgag
   121321 gttcttaagc aatcgtcctt gcttgaccca acattaatgc tgttggcctt tgtgtttgtg
   121381 ctaattggct ttggtaccaa aaccgggcta tttcccatgc acgcctggct gccggatgct
   121441 cacagtgaag cgccgagtcc ggtcagcgcc ctgctctccg ccgtattgct gaactgcgcg
   121501 ctgttggtgc tgattcgcta ttacatcatt atttgccagg ccatcggcag cgatttcccc
   121561 aaccggttgt tgctcatctt cggcatgttg tcggttgccg tggcggcatt tttcattctg
   121621 gtacagcggg acattaagcg tctgctggcg tactccagcg tggagaacat ggggctggtc
   121681 gcggtggcgc taggcattgg cgggccgctg ggaatttttg ccgcgctgct gcacacctta
   121741 aaccacagtc tggcaaaaac gctgctgttc tgcggttccg gcaatgtact gctcaagtac
   121801 ggcacgcgcg atctcaacgt cgtctgtggg atgctcaaaa tcatgccatt taccgccgtg
   121861 ctgtttggcg gcggtgcgct ggcgctggca gggatgccgc ccttcaacat ttttcttagc
   121921 gaatttatga ccgttaccgc aggactggca cgtaatcacc tgctgattat cgtcctgctg
   121981 ttattgctgt taacgctggt gctcgcgggc ctggtacgga tggctgcgcg ggtgttaatg
   122041 gcgaaaccgc cgcaggccgt taaccggggt gatctcggct ggttgaccac ctcgccaatg
   122101 gtgattctgc tagtcatgat gctggcgatg ggaacgcata ttccacaacc tgtcatcagg
   122161 atcctggcgg gcgcttccac tatagtcctc tcagggacgc acgacctgcc tgcacaacgt
   122221 agcacctggc atgatttttt gccttcaggc accgcatctg tttcggagaa acacagtgaa
   122281 cgttaattca tcgtcaaatc gtggcgaagc gattctcgcc gccctgaaaa cgcagttccc
   122341 cggcgcggtg ctggatgaag agcgacaaac gcctgaacag gtcaccatta cggtgaaaat
   122401 caatctgctg cctgacgttg tacattatct ttattatcaa catgatggct ggcttccagt
   122461 cctgtttggc aacgacgagc ggacacttaa cggtcattac gcggtttatt atgccctttc
   122521 aatggaaggg gccgaaaaat gctggatcgt ggtgaaggca ctggtcgatg ccgacagtcg
   122581 ggagtttccg tcagtcacac cgcgcgtccc tgccgcggtc tggggcgagc gagaaattcg
   122641 cgatatgtac gggctgattc cggttggcct gccggatcag cgtcgcctgg tgttgcccga
   122701 tgactggccg gaagatatgc atccgctgcg caaagatgcg atggattatc gactgcgccc
   122761 tgaaccgacg acagattccg aaacgtatgc gtttatcaat gagggcaaca gcgatgcgcg
   122821 ggtgatccct gtcggcccgc tgcatatcac ctcctatgaa ccgggtcact tccgcttgtt
   122881 tgtggatggc gagcaaattg tcgatgctga ttaccgcctg ttttatgtcc atcgcggcat
   122941 ggagaaactg gcagaaacgc ggatgggcta caacgaagtg accttcttat cggaccgcgt
   123001 gtgtgggatt tgcggttttg cccacagtgt ggcctatacc aactcggttg aaaatgcact
   123061 ggggattgag gtgccgcaac gagcacatac tattcgctcg attctgctgg aagtcgagcg
   123121 gctacacagt catttgctca accttggcct ctcctgccat tttgttggtt ttgataccgg
   123181 ctttatgcaa tttttccgcg tgcgggaaaa gtcgatgacg atggcggaat tgctgaccgg
   123241 gtcgcgtaaa acctacggtc tgaatctgat tggtggtgtt cgccgcgata ttctcaaaga
   123301 gcaacgtctg caaacgctga aactggtgcg cgagatgcgc gccgacgtgt cggagctggt
   123361 agagatgctg cttgctacgc cgaatatgga acaacgcact cagggcattg gcattctcga
   123421 ccgacaaatc gcccgtgatt atagccctgt agggccgctg atccgcgcag tggttttgcc
   123481 cgtgatttgc gctttgatca cccctacgcc gactacggca atattccaaa aacactgttt
   123541 acctttaccg gcggcgatgt tttctcccgc gtgatggtcc gtgtcaaaga gacgtttgat
   123601 tcgctggcaa tgctggaatt tgccctcgac aacatgccgg ataccccact gctgaccgaa
   123661 ggctttagct ataaacctca cgcattcgcg ctgggctttg ttgaagcgcc acgcggtgaa
   123721 gacgtgcact ggagcatgct cggtgataac caaaaattgt tccgctggcg ctgtcgtgcc
   123781 gccacctacg ccaactggcc ggtgttgcgt tacatgctgc gcggcaatac cgtttctgac
   123841 gcaccgctga ttatcggtag ccttgatccc tgctactcct gtaccgaccg tgtgacgctg
   123901 gtagatgtgc gcaagcgcca gtcaaaaacc gtgctgtata aagagatcga acgctacggc
   123961 attgatcgta accgttcgcc gctgaagtaa ggacagaaga tgctgaagtt actgaaaact
   124021 attatgcgcg ccggaacccc gacggtgaaa tatcccttcg cgccactgga ggtcagccca
   124081 ggctttcgcg ggaaaccgga cctgatgccc agccaatgta ttgcctgcgg tgcctgcgcc
   124141 tgtgcttgtc cggcaaatgc gctgactatc cagaccgacg accagcaaaa ttcgcgcacc
   124201 tggcagctct atctggggcg ttgtatttac tgcggacgtt gtgaagaagt gtgcccgacc
   124261 agagccatcc agcttaccaa taactttgaa ctgaccgtca cccataaagc cgatctctat
   124321 acccacgcga cgttccatct acaacgttgc agccgttgcg aacgcccgtt tgccccgcaa
   124381 aaaaccgtcg cactggctgc tgaattgttg gcacagcaac aaaatgctcc acaaaaccgc
   124441 gaaatgttat gggcgcaagc gagcgtctgc ccggagtgca aacaacgcgc gacgctgatc
   124501 aacgacgata cagatgtacc gctggtggct aaggagcagc tatgagtcca gtgcttacgc
   124561 aatatgtcag ccagcccatc acgctggacg agcaaacgca aaagatgaag cggcatttgc
   124621 tacaggatat ccgtcgctcg gcttacgttt atcgcatcga ttgcggcggc tgcaacgcct
   124681 gtgaaatcga aatttttgct gccatcaccc ccgtattcga cgcagaacgt tttggcatta
   124741 aggttgtttc atcaccgcgt cacgccgata ttttgttatt tactggcgca gtcacccggg
   124801 cgatgcgtat gcctgcactt cgggcgtatg agtctgcccc cgatcataaa atctgtgttt
   124861 cctacggcgc gtgcggtgtc ggcggcggta ttttccacga tctctacagc gtctggggcg
   124921 gcagcgacac cattgtcccc attgatgttt ggatccccgg ctgcccgcca acaccggccg
   124981 ccaccattca cggtttcgcc gtggcgctcg gtttgctgca acagaagatt cacgcggtgg
   125041 attatcgcga tcccaccggg gtgactatgc aaccgttgtg gccgcagatc ccgccatcac
   125101 agcgtatcgc cattgagcga gaagcgcggc ggctggcggg ctatcgtcag gggcgagaaa
   125161 tttgcgatcg gctcctgcgc catttaagcg acgatcctac aggaaatcgg gttaacacct
   125221 ggttgcgcga tgccgacgat ccacgtctca atagtatcgt tcagcaactc tttcgcgtac
   125281 tccgggggtt acatgactga agagtgcggg gaaattgttt tctggacgct gcgaaaaaag
   125341 tttgtcgcca gtagcgacga gatgccggaa cacagctctc aggtaatgta ttactcgctg
   125401 gctatcggtc atcacgttgg cgtgattgat tgtctgaatg tcgccttccg ctgcccactg
   125461 acggaatacg aagattggct tgcactggtc gaagaggagc aagcccgacg taagatgctg
   125521 ggggtgatga cttttggtga gattgttatt gacgccagcc acaccgccct gttgacccgg
   125581 gcattcgcgc cactggcaga tgacgcgacg tctgtgtggc aagcgcgtag cattcaattc
   125641 attcatctgt tggatgaaat tgtgctggaa ccggctatct atctgatggc cagaaaaatt
   125701 gcgtgagaag gatttctcat taataaggac tgttgatggc tatgtcagac gaagcgatgt
   125761 ttgccccgcc gcaaggaata acaattgaag cggtaaacgg aatgctcgcg gagcggttag
   125821 cgcagaaaca cggcaaggcg tctttattac gcgccttcat cccgctgccg ccgccgttca
   125881 gcccggtaca acttattgaa ctgcatgttc tcaaaagcaa cttctattac cgctaccatg
   125941 atgatggcag cgatgtgacg gcaacaacag agtatcaggg cgagatggtc gattattcgc
   126001 gtcacgccgt ccttctcggc agtagttgaa tggcggagct acgctttatt cgtacccacg
   126061 gcagccgttt tactccccag gattgcacac tgtttaactg gctggcacgc attatcaccc
   126121 cggttctgca atcatggctc aatgatgaag aacagcaggt ggcgctgcgt ttgctggaga
   126181 aagatcgcga tcatcatcgg gtactggtgg atatcactaa tgcagtgctg tcacatcttg
   126241 atctcgacga tctgatcgct gacgtcgctc gtgagatcca tcattttttc ggtctggctt
   126301 cagtcagtat ggtactgggc gatcatcgaa agaacgagaa gttcagcctg tggtgcagcg
   126361 atctttctgc ctcacattgt gcgtgtctgc cacacaatat gcctggcgac agtgtattgc
   126421 tgacacaaac gctacaaacc cgacaaccga ccttgacgca ccgtgcagat gatctgtttc
   126481 tctggcaacg cgacccgtta ttactcttac ttgcatctaa cggctgcgaa tctgcgctcc
   126541 ttataccgct tacctttggc aaccatacac cgggtgcatt gttgctggcg catacctctt
   126601 ccactctctt tagtgaggaa aactgccagc tactacaaca catagccgat cgcatcgcta
   126661 ttgccgttgg caatgccgat gcctgccgta gaatgaccga tttgcaggaa agtttgcagc
   126721 aagaaaacca ccagcttagc gagcagctcc tttcgaatct gggcgtcggt gacattatct
   126781 atcaaagcca ggcaatggaa gacctgctcc agcaggtaga tattgtggcg aagagcgaca
   126841 gtacggtgtt gatttgcggt gaaaccggaa ccggcaaaga ggtgatcgcc agagcgatcc
   126901 atcaacttag cccgcgacac gacaagccgc tggtcaaaat caactgcgct gccatccccg
   126961 ccagtcttct ggaaagtgag ttattcggtc atgacaaagg ggcgtttact ggtgcgatta
   127021 atacccatcg tggtcgtttt gaaattgccg atggcggcac gttgtttctc gatgaaattg
   127081 gcgatctgcc gttagaactt cagcctaaac tgctgcgcgt attgcaggaa cgggagattg
   127141 agcgtctcgg cgggagtaga acgatcccgg taaatgtcag agtcattgcc gccaccaacc
   127201 gtgatttgtg gcaaatggtt gaagatcgcc agtttcgcag cgatctcttt tatcgcctga
   127261 atgtcttccc actggaattg cctccgctgc gcgaccgtcc ggaagatatc cctcttttag
   127321 caaagcattt cacgcaaaaa atggcgcgcc atatgaatcg ttcaattgac gccatcccga
   127381 ccgaggcgct acgccagttg atgtcgtggg actggccggg caacgtgcgc gagctggaaa
   127441 acgtgattga gcgagcggta ctgctgactc gcggtaacag tctgaattta catcttaatg
   127501 tccgacaaag ccgtttactg ccgacgctaa atgaagattc agcgcttcgc agttcaatgg
   127561 cgcagttgct gcacccgacg acgccagaga atgacgaaga agaacgtcag cgcattgttc
   127621 aggtattgcg agaaaccaat ggcattgttg ccgggccccg tggcgcggcg acacgattag
   127681 ggatgaagcg caccacgctg ctgtcacgaa tgcagcgtct gggaatctcg gttcgcgagg
   127741 tgttgtaatc tgcttttgca ggagtatgca tgagaaacaa actctctttc gacttgcagt
   127801 tgagcgccag aaaagcggca atcgctgaac ggattgccgc ccataaaatt gcccgcagta
   127861 aagtgtcggt ctttttaatg gcgatgtccg ctggcgtgtt tatggcgatc ggatttactt
   127921 tttacctttc cgttatcgcc gatgccccgt cttcataggc attaacccat ctggtgggcg
   127981 gcctttgctt tacacttggc tttattttgc tggcggtttg cggcaccagc ctgttcacct
   128041 cgtcggtaat gacggtaatg gcaaaaagtc ggggcgttat tagttggcga acttggctga
   128101 ttaacgcact tctggtggcc tgcggtaatc tggcaggtat tgcctgtttc agtttgttaa
   128161 tctggttttc cgggctggtg atgagtgaaa acgcgatgtg gggagtcgcg attttacact
   128221 gcgccgaggg caaaatgcat catacattta ctgaatctgt cagcctcggc attatgtgca
   128281 atctgatggt ttgcctggcg ctgtggatga gttattgcgg acgttcgtta tgcgacaaaa
   128341 tcgtcgccat gattttgtcc atcaccctgt ttgtcgccag tggctttgag cactgtatcg
   128401 ccaatttgtt tgtgattccg ttcgccattg ccattcgcca tttcgcccct acctccttct
   128461 ggcaactggc gcacagtagc gcagacaatt ttccggcact gacggtcagc cattttatta
   128521 ccgccaatct gctcccggtg atgctgggta atattatcgg cggtgcagtg ctggtgagta
   128581 tttgttatcg ggctatttat ttacgtcagg aatcctgaaa aatcagcccg gcgaaacagt
   128641 tcgtcgggct gaagattatt cttgcgcgat ttgcccatcg ggccaggcgt ggatcacggc
   128701 tttgatcagc gtcgccaatg gaatggcgaa gaatacgccc cagaatcccc acaaaccacc
   128761 gaagatcacc accgataaaa taatcaccag cggatgcagg ttaaccgctt cggagaacaa
   128821 caccggtact agcaggttgc cgtccagcgc ctgaataatc agatacaccg cgaagcagct
   128881 ccagaattca gtacctgcgc caaactggaa tagcgccacg ccaaccaccg gaatggtcac
   128941 cacaaatgcg ccaatgtacg gaataagaac cgagaagccg accagcactg ccagcagcag
   129001 cgaatagttc agcccgaaga gcaagaaccc cagccaggtg gcgatcccca ccacgatcat
   129061 ctccagcact ttgccgcgga tatagttggt gatttgttga ttcatctcct tccacacctg
   129121 tcctgccagc ccacggttac gcggcagcac ccggcgaacg gcgttcagca tctgctcttt
   129181 gtctttcagc aggaagaaga ccatcaatgg caccagcacc agatagacgg ctatggtcag
   129241 caaaccgacc agcgaggcga gggaaatttt caccaccgaa tcgcccatgg tcagcatccg
   129301 actgcgcata ttttcggcca ttgcatcaat aatgcccgcg tccattaaca ccggatagcg
   129361 gcgcggcaac gtggcggcaa agtcagaaag cttattgagc atccccggca tatcgcggat
   129421 taagtagatg ccctgttgcc aggcgatagg cagtaccacg aacgccatca gtagcaatat
   129481 accgacaaaa accaccaata caatcgacgt cgcccagcgg cgggagcagc caatagattg
   129541 caggcgcacg gttggccatt ccagcaaata agccagcaca atagccacca gcaacggagc
   129601 aagcaggcca ctaaagaaaa agataatgcc aaatccggca actaaaataa ccagcaaggc
   129661 aatcgcttcc gggtcgctaa aacggcggcg ataccattgc atcaacattt cgagcataaa
   129721 gaccttccct gaacctcaag agcgggattg cgatccgcaa ttgtatcgaa atgtcacaaa
   129781 aaagacttcg ctttttatga cggattcagg aaactgaaaa gtcatttgag tgggctaatc
   129841 ttcgccgtta cactcaaagg cggcgaggtg ggaacgatat ttcacagtat cggtcaaatg
   129901 actatcttca gaaatacagg atagaggtta tgttcaggca gttgaaaaaa aacctggttg
   129961 caaccctcat tgctgctatg accattggtc aggtagcccc ggcgtttgcc gacagcgcag
   130021 acaccttgcc ggatatggga acctccgcag gaagcacgct ttccattggt caggaaatgc
   130081 agatgggcga ctattatgtc cgccagctac gcggcagcgc gccgttaatt aatgacccgc
   130141 tgttaacgca atatattaat tcgctgggga tgcgtctggt ttcgcatgcc aattcggtta
   130201 agacaccgtt tcattttttt ctgatcaaca acgacgaaat taacgccttt gctttctttg
   130261 gcggcaacgt ggtgctgcac tctgccctgt tccgttattc cgataacgaa agtcaactgg
   130321 cttcagttat ggcgcacgaa atctcccacg tcacccaacg tcacctggcg cgagcgatgg
   130381 aagatcaaca acgcagcgcg ccgctgacct gggtcggcgc gttaggttct attttactgg
   130441 cgatggccag tccgcaggcg gggatggcgg cgctgaccgg tacactggcg ggaacgcgcc
   130501 aggggatgat cagtttcacc cagcaaaatg aacaggaagc ggaccgcatt ggtattcagg
   130561 tgctgcaacg ctcgggattc gatccacagg cgatgccgac cttcctggaa aaattgctcg
   130621 atcaggcgcg ttactcctcg cgcccgccgg aaattttatt gactcacccg ttgccggaaa
   130681 gtcgtctggc agatgcccgc aaccgtgcta atcagatgcg cccgatggtg gtgcagtcgt
   130741 cggaagattt ctatctggcg aaagcgcgca cactggggat gtataattcc ggacgtaacc
   130801 agctcaccag tgatttgctg gatgaatggg cgaaaggaaa cgttcttcag caacgagcgg
   130861 cgcaatatgg tcgtgcttta caggcgatgg aagccaataa atacgacgag gcgcgtaaaa
   130921 cgctgcaacc gttactggcg gcagaacctg gcaacgcatg gtatctcgat ctggctaccg
   130981 atatcgatct tgagcaaaac aaagccaatg aggcaatcaa tcgcctgaaa aatgcccgcg
   131041 atttacgcac caatccggtg ttgcagctca acctggcgaa cgcttatctg caaggcggtc
   131101 aaccacaaga agcggccaat attctgaatc gctacacctt taataataaa gatgacagca
   131161 acggctggga tttgctggca caggcggaag ccgcgctaaa taaccgcgat caggagctgg
   131221 ctgcgcgagc agaaggttat gcgctcgccg gacgactcga tcaggccatt tcgctgttga
   131281 gtagcgccag ttcgcaggtg aaattaggca gcctgcaaca agcgcgttac gatgcgcgca
   131341 tcgaccagtt gcgccagctg caggaacgct ttaagcctta taccaagatg taattcgcat
   131401 taaaggaaga acaatgacca aacaggtaaa aatctaccat aacccacgct gttcaaagag
   131461 ccgggaaacg ctgaacctgc tgaaagaaaa cggcgtggag ccagaagtgg tgctctacct
   131521 tgagacaccc gccgatgcgg caacgctgcg cgatttgctg aaaatgctgg ggatgaatag
   131581 cgcccgtgaa ttgatgcgcc agaaagagga tctttataaa gaactaaacc tggctgacag
   131641 ttcgcttagc gaagaggcgc tgattcaggc aatggtggat aatccgaagc tgatggagcg
   131701 cccgattatg gtcgcgaatg gcaaagcgcg gattggtcgt ccaccggagc aggtactgga
   131761 aatcgtgggt taagatgtgc tccatcgcta gttgaagcac attgccggat gcgacgcttg
   131821 ccacgtctta tccggcctac gaatactatg gattaacttc gcgtaggtca ggcgttcgcg
   131881 ccgcatccga caataaacac cttatctaca acttcagaat ttctttcaca aacggaatgg
   131941 tcagcttacg ttgcgcggta atcgacgcac gatccaactg atccaacgtc ataaatagcg
   132001 tgcgcatttc tctgtcgagc cgcttcagca agaaacgccc cacatcttcc ggcagttcaa
   132061 aaccacgcaa acgcgcgcgt aactgtagcg cctgcaactt atcttcatca gaaagtggct
   132121 gcaatttgta gatctgcccc cagtcgagac gcgacgcgag atccggtaat cccagattca
   132181 actgccgcgg tggacgatcg ccggtgatca acaaccgtgt tttgcccgat tccagaattc
   132241 gattgtagag atcgaaaatc gccatctccc acaactcatc gcctgcaata cactcaatgt
   132301 tgtcgataca gaccagcgac aaatgctcca taccgtcgag cacttccgga acaaaccagg
   132361 tgcgtttatc cagcgggaca tagcccaccg catcgccacg ctgcgacaat tccgcgcaag
   132421 ccgcgtgcag cagatggctg cgccccgcgc cttcgcgtgc ccagagatag atgtaaccgc
   132481 tatgttcctg acgcagcacg ttttgcagcg cggccagtaa agaggagtta tcccccggcc
   132541 agaaacttgc aaaggtttcg tcgtcaggaa gataaagtgg caaagagagc tgtgccggtg
   132601 tgttcagaga tacctcaacc aggatttcac aaaatcgcga gaagtttacc acagaatccc
   132661 atgatgtttg aaccgggcag caacactgcc cggtcggtac attatttgtc tgttatatcc
   132721 gcgtcttctg cgtccagcac cacttcttca gggcgcagca cactgatcag tttgaaaatc
   132781 aaacttaagc cgataccgac gatggtcgcc agcgccatac cttttaactc cgccgcgccg
   132841 atgtttacct tcgcgccact gacgccgatg atcaaaatca cggaagtcag gatcaggttc
   132901 tgtgctttgt tgtaatccac tttcgattcg atcaaaacac gaataccgga agcaccaatg
   132961 acaccataaa gcagcagcga aacgccgccc atcaccggca atgggatcat ctggatagcg
   133021 gcagccagtt taccgacgca ggaaagcagg atagcgaaaa tcgccgcccc gccgataacc
   133081 caggtactgt aaacacgggt gatcgccatc acgccaatgt tttctccgta agtggtattt
   133141 ggcgtagagc caaagaagcc ggagatcacg gtcgacaggc cgttagcaaa catcgaacgg
   133201 tgcagacctg gatcgcgcag cagatctttt ttgacgatat tagccgttac taccaggtgc
   133261 cctacgtgtt cggcaataac cactaacgcc gctggcagaa tagtcagaat ggcaaaccac
   133321 tcgaagcgcg gcgtatagag ggttggcagt gcaaaccagt gagcattaat aatcggcgtg
   133381 gtatcgacaa ttcccattgc gaaagagagc gcgtacccca ccagcacgcc aattaaaatc
   133441 gggataattg ccaggaaacc acgaaacagc acggaaccta aaaccgtgac cgccagggtg
   133501 gtaatagaga tgatgatggt tttggagttt ggcgtttgcc cttcagccgg gagtaaaccc
   133561 gccataccgg cagctacgcc cgccagctcc agaccgatga cggcaacgat tgcgcccatt
   133621 gccgcaggtg gaaacagcac gtccaaccag ccggtccccg ctttcttcac gataaaagaa
   133681 accaggcaga acagcacgcc gcacataata aagccgccca gcgcgacttc ataccctaac
   133741 ggcaacagta acaataccgg tgaaataaag gcaaagctgg aaccaagata agccggaatt
   133801 ttccctttac agatgaagag atacagcagc gttccaatac cgttaaataa cagtacagtc
   133861 gccgggttaa tatgaaataa gacgggcacc aggacggttg caccaaacat ggcgaacaaa
   133921 tgttgcaaac taagcgggat tgtctgtaaa agtggcggtc tttcactcac cccgatagca
   133981 cggcgcgtca tagtattatc ctctgtatta tgtgttatag gcgctttact caaaaaaaag
   134041 ccgactctta aagtcggctt taattatttt tattctttat ttcgtaccaa agattttgtc
   134101 accggcatcg ccgaggcccg gaataatgta tccgtgctcg ttcagtccct ggtcaatcga
   134161 tgcagtatac agttcgacgt ccgggtgcgc tttttccagc gcagcgatac cttctggcgc
   134221 agcgaccagc accagaactt tgatgctgct gcagcccgct tttttcagca ggtcgatggt
   134281 cgcgataacg gaaccaccgg ttgccagcat tgggtcaacg atcagcgcca tacgctcatc
   134341 gatgttagaa accagtttct ggaagtacgg taccggctcc agcgtttctt cattacggta
   134401 cataccgaca acgctgatgc gcgcgctcgg aacgttttcc agcacaccgt ccatcatacc
   134461 aagacccgca cgcagaattg gcacaacggt aattttctta cctttgatct ggtcgatttc
   134521 taccgggccg ttccagcctt cgatagttac tttttccgtt tcgaggtcgg cggtcgcttc
   134581 gtaagtcagc aggctaccca cttcggaagc gagttcgcga aagcgcttgg tgctgatatc
   134641 ttgctcacgc atcagtccca gcttgtgttt gacgagtggg tgtttgactt ccacgatctt
   134701 catactcttt ctcctttgag gggcagccac aaaaaaaatc gacggattat acctcctttc
   134761 ttcaaggcgg caatattatt ttcgttgact ttagtcaaaa tgataacagt ttgagataaa
   134821 gttattttat attcaaatgg ttatgaaaga agattattcc atccgaaaac taacctttgc
   134881 cctggcacaa gtcttctttc gccgcgcgcc cggggaaaag acgtgcaaaa aggttgtgta
   134941 aaacagtctc gcaaacgttt gctttccctg ttagaattgc gccgaatttt atttttctac
   135001 cgcaagtaac gcgtggggac ccaagcagtg accgataaaa cctctcttag ctacaaagat
   135061 gccggtgttg atattgacgc gggtaatgct ctggttggaa gaatcaaagg cgtagtgaag
   135121 aaaacgcgtc gtccggaagt gatgggcggt ctgggcggct tcggtgcgct gtgtgcattg
   135181 ccgcaaaaat atcgtgaacc cgtgctggtt tccggcactg acggcgtagg taccaagctg
   135241 cgtctggcaa tggacttaaa acgtcacgac accattggta ttgatctggt cgccatgtgc
   135301 gttaatgacc tggtggtgca aggtgcggaa ccgctgtttt tcctcgacta ttacgcaacc
   135361 ggaaaactgg atgttgatac cgcttcagca gtgatcagcg gcatcgcgga aggttgtctg
   135421 caatcgggct gttctctggt gggtggcgaa acggcagaaa tgccggggat gtatcacggt
   135481 gaagattacg atgtcgcggg tttctgcgtg ggcgtggtag aaaaatcaga aatcatcgac
   135541 ggctctaaag tcagcgacgg cgatgtgctg attgcactcg gttccagcgg tccgcactcg
   135601 aacggctatt cgctggtgcg caaaattctt gaagtcagcg gttgtgatcc gcaaaccacc
   135661 gaacttgatg gtaagccatt agccgatcat ctgctggcac cgacccgcat ttacgtgaag
   135721 tcagtgctgg agttgattga aaaggtcgat gtgcatgcca ttgcgcacct gaccggcggc
   135781 ggcttctggg aaaacattcc gcgcgtattg ccagataata cccaggcagt gattgatgaa
   135841 tcttcctggc agtggccgga agtgttcaac tggctgcaaa cggcaggtaa cgttgagcgc
   135901 catgaaatgt atcgcacctt caactgcggc gtcgggatga ttatcgccct acctgctccg
   135961 gaagtggaca aagccctcgc cctgctaaat gccaacggtg aaaacgcgtg gaaaatcggt
   136021 atcatcaaag cctctgattc cgaacaacgc gtggttatcg aataatgaat attgtggtgc
   136081 ttatttccgg caacggaagt aatttacagg caattattga cgcctgtaaa accaacaaaa
   136141 ttaaaggcac cgtacgggca gttttcagca ataaggccga cgcgttcggc cttgaacgcg
   136201 cccgccaggc gggtattgca acgcatacgc tcatcgccag cgcgtttgac agtcgtgaag
   136261 cctatgaccg ggagttgatt catgaaatcg acatgtacgc acccgatgtg gtcgtgctgg
   136321 ctggttttat gcgcattctc agcccggcgt ttgtctccca ctatgccggg cgtttgctga
   136381 acattcaccc ttctctgctg ccgaaatatc ccggattaca cacccatcgt caggcgctgg
   136441 aaaatggcga tgaagagcac ggtacatcgg tgcatttcgt caccgatgaa ctggacggtg
   136501 gcccggttat tttacaggcg aaagtcccgg tatttgctgg tgatacggaa gatgacatca
   136561 ccgcccgcgt gcaaacccag gaacacgcta tttatccact ggtgattagc tggtttgccg
   136621 atggtcgtct gaaaatgcac gaaaacgccg cgtggctgga tggtcaacgt ctgccgccgc
   136681 agagctacgc tgccgacgag taatgccccc gtagttaaag cgccagctct gccgctggcg
   136741 tttttcaatt cacctgtaaa tcgcaagctc cagcagtttt tttccccctt ttctggcata
   136801 gttggacatc tgccaatatt gctcgccata atatccaggc agtgtcccgt gaataaaacg
   136861 gagtaaaagt ggtaatgggt caggaaaagc tatacatcga aaaagagctc agttggttat
   136921 cgttcaatga acgcgtgctt caggaagcgg cggacaaatc taacccgctg attgaaagga
   136981 tgcgtttcct ggggatctat tccaataacc ttgatgagtt ctataaagtc cgcttcgctg
   137041 aactgaagcg acgcatcatt attagcgaag aacaaggctc caacgctcat tcccgccatt
   137101 tactgggcaa aattcagtcc cgggtgctga aagccgatca ggaattcgac ggcctctaca
   137161 acgagctatt gctggagatg gcgcgcaacc agatcttcct gattaatgaa cgccagctct
   137221 ccgtcaatca acaaaactgg ctgcgtcatt attttaagca gtatctgcgt cagcacatta
   137281 cgccgatttt aatcaatcct gacactgact tagtgcagtt cctgaaagat gattacacct
   137341 atctggcggt ggaaattatc cgtagcgata gcatccgtta cgcgctgctg gagatcccat
   137401 cagataaagt gccgcgcttt gtgaatttac cgccagaagc gccgcgtcga cgcaagccga
   137461 tgattcttct ggataacatt ctgcgttact gccttgatga tattttcaaa ggcttctttg
   137521 attatgacgc gctgaatgcc tattcaatga agatgacccg cgatgccgaa tacgatttag
   137581 tgcatgagat ggaagccagc ctgatggagt tgatgtcttc cagtctcaag cagcgtttaa
   137641 ctgctgagcc ggtgcgtttt gtttatcagc gcgatatgcc caatgcgctg gttgaagtgt
   137701 tacgcgaaaa actgactatt tcccgctacg actccatcgt ccccggcggt cgttatcata
   137761 attttaaaga ctttattaat tttcccaatg tcggcaaagc caatctggtg aacaaaccac
   137821 tgccgcgttt acgccatatt tggtttgata aagcccagtt ccgcaatggt tttgatgcca
   137881 ttcgcgaacg cgatgtgttg ctctattatc cttatcacac ctttgggcat gtgctggaac
   137941 tgctgcgtca ggcttcgttc gacccgagcg tactggcgat taaaattaac atttaccgcg
   138001 tggcgaaaga ttcacgcatc atcgactcga tgatccacgc cgcacataac ggtaagaaag
   138061 tcactgtggt ggttgagtta caggcgcgtt tcgacgaaga agccaacatt cactgggcga
   138121 agcgcctgac cgaagcaggc gtgcacgtta tcttctctgc gccggggctg aaaattcacg
   138181 ccaaactgtt cctgatttca cgtaaagaaa acggtgaagt ggtccgttac gcacacatcg
   138241 ggaccgggaa ctttaacgaa aaaaccgcgc gtctttatac tgactattcg ttgctgaccg
   138301 cagatgcgcg tatcaccaac gaagtacggc gggtatttaa ctttattgaa aacccatacc
   138361 gcccggtgac atttgattat ttaatggtgt caccgcaaaa ctctcgccgt ctgttatatg
   138421 aaatggtaga ccgcgaaatc gccaacgcgc agcaagggct gcccagtggt atcaccctga
   138481 agctaaataa ccttgtcgat aaaggcctgg ttgatcgtct gtatgcggcc tccagctccg
   138541 gcgtaccggt taatctgctg gttcgcggaa tgtgttcgct gatccccaat ctggaaggca
   138601 ttagcgacaa cattcgtgcc atcagtattg ttgaccgtta ccttgaacat gaccgggttt
   138661 atatttttga aaatggcggc gataaaaagg tctacctttc ttccgccgac tggatgacgc
   138721 gcaatattga ttatcgtatt gaagtggcga caccgctgct cgatccgcgc ctgaagcagc
   138781 gggtgctgga catcatcgac atattgttca gcgatacggt caaagcacgt tatatcgata
   138841 aagaactcag taatcgctac gttccccgcg gcaatcgccg caaagtacgg gcgcagttgg
   138901 cgatttacga ctacatcaaa tcactcgaac aacctgaata accctatgcc aatacacgat
   138961 aaatcccctc gtccgcagga gtttgctgcg gtcgatcttg gttcaaacag ttttcacatg
   139021 gtcatagccc gtgtggtaga tggtgccatg cagattattg gccgcctgaa acagcgggtg
   139081 catctggcgg acggcctggg gccagataat atgttgagtg aagaggcaat gacgcgcggt
   139141 ttgaactgtc tgtcgctgtt tgccgaacgg ctacaagggt tttctcctgc cagcgtctgt
   139201 atagtcggca cccatacgct gcgtcaggcg ctgaacgcca ctgactttct gaaacgcgcg
   139261 gaaaaggtca ttccctaccc gattgaaatt atttccggta atgaagaagc ccgtctgatt
   139321 tttatgggcg tggaacatac ccaaccggaa aaaggtcgca aactggttat tgatattggc
   139381 ggcggatcta cggaactggt gattggtgaa aatttcgaac ctattctcgt tgaaagccgc
   139441 cggatgggtt gtgtcagctt tgcccagctt tattttcctg gcggggtcat caataaagag
   139501 aattttcagc gcgctcgcat ggcggcagca caaaaactgg aaactttaac ctggcaattc
   139561 cgtattcagg gctggaacgt tgcaatgggc gcttccggta ccataaaagc cgcccatgaa
   139621 gtgttaatgg aaatgggcga gaaagacggg ataattaccc cggaacgtct ggaaaaactg
   139681 gtaaaagaaa ttttacgtca ccgtaatttc gcatcgctga gtttaccggg tctttccgaa
   139741 gagcggaaaa cagtcttcgt tcctggactg gcgattttat gtggtgtgtt tgatgcttta
   139801 gccatccgtg aactgcgcct ttctgacggg gcgttacgcg aaggcgtact gtatgaaatg
   139861 gaaggacgtt tccgtcatca ggatgtgcgt agtcgcaccg ccagcagcct cgccaaccag
   139921 tatcacatag acagcgaaca ggcccgacga gtgctggata ccactatgca aatgtacgaa
   139981 cagtggcggg aacagcaacc gaagctggcg catccgcaac tggaggcgct actgcgatgg
   140041 gccgccatgc tgcatgaggt cgggttgaat atcaaccaca gcggtttgca tcgccactcc
   140101 gcttatattc tgcaaaacag tgacttgccg ggttttaatc aggaacagca gctgatgatg
   140161 gcgacactgc tgcgctatca ccgtaaagcg attaagctcg acgatctgcc gcgctttacc
   140221 ttgtttaaga agaaacagtt cctgccactg atacagctat tgcgccttgg cgtattacta
   140281 aacaatcaac gtcaggcaac caccacaccg ccaacattga cactgattac tgatgacagt
   140341 cactggacac tgcgtttccc gcatgactgg tttagtcaga atgcgctggt actgcttgat
   140401 ctggaaaagg agcaagaata ctgggaaggc gtggctggct ggcggttgaa aattgaagaa
   140461 gaaagtaccc cagaaatcgc cgcttaatgc tttgcgggcc gacgagaaat gtcggcccgc
   140521 attattcagg cactttcgcg aatgggttcg atttcattca gcgtatcaat taacggttgc
   140581 ggcttaccaa taagataacc ctgcatataa tcgatcccca aagagagcac cgcctcgcgg
   140641 atctcttcgt tttcaacgta ctctgccact acccgcattt tcttcattcg cgccagatgg
   140701 caaatcgacg ccacaatctg ataatccaga ctatttgaca caatattgcg gataaagctg
   140761 ccgtcaattt taagcagatc ggcattcaca tttttaagcc gtgcatagct ggcgtagccg
   140821 gtgccgaaat catcaatcgc aatctggcag cctaattcct gaagatgctg caaggtgatt
   140881 tgcgcctgct taacattggt cagagcatta ctttcggtga cttcaaaaat aagttgccac
   140941 gcttcaatct gatatttagc cagcaactga ctgacttcaa caggaaaacg agcctgacat
   141001 accgaggttg gagacagatt aatagcaaaa cggtgagcgg gcatcttcgc tctgttttca
   141061 gccataaatt gcagcgtatg ctcaatgacc cacatgtcga tactcgacga taaaccaaat
   141121 tcgtgcgcga ccggcaagaa gctatcgggg ccgatcagtt catcattctc acctttcatg
   141181 cgcagaagaa tttcatggta aacatcacca cgcataccgg taatcggctg ggccatcagg
   141241 aaaaaatggt tgtgctccag cgcctgctgt agtcgattca tcatcgcgac tttatctttc
   141301 aattcgcgtt gcaaatacat tgccccgcaa cgctgcatat tttccggggc gttggtcacg
   141361 atggaaagtt cggcgaccgt atttagctct cccagcagca ggtagatatg attcactggc
   141421 gagcgcacat agcagtaact gacgccaatc tgcggttgca tcggcatccc atcccaaaag
   141481 aaacgaaatt gcttgagatg actatccagt gcggtaatgc gctcctggtg cgattctgta
   141541 ttcagtcgca gcgcgagatc gttacccgaa agctgataaa catcttcacc cggttccagc
   141601 aagggtgaca gccagtgaga aagtttttgc ttgtattgag gtagcctgag tttaacggac
   141661 actccttcct gaaatagaat ggcatcagaa ggagctaata atgagcagaa aaacccaacg
   141721 ttactctaaa gagttcaaag ccgaagctgt cagaacggtt cttgaaaatc aactttcgat
   141781 cagtgaaggc gcttcccgat tatcccttcc tgaaggcact ttaggacaat aggttaccgc
   141841 cgccagaaaa gggctcggta ctcctggttc ccgcacggtg gctgaactgg aatctgaaat
   141901 tctgcaactg cgtaaggcgt taaatgaagc tcgccttgag c
//
LOCUS       DAETPZ010000002        99081 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.002, whole genome shotgun sequence.
ACCESSION   DAETPZ010000002 DAETPZ010000000
VERSION     DAETPZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 99081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 99081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..99081
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.002"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..105)
                     /locus_tag="K0W59_000135"
     CDS             complement(<1..105)
                     /locus_tag="K0W59_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBI3021319.1"
                     /translation="MNSQTTKDIPCFRSYLPDALHLRFEDKLTIRAIAQ"
     gene            complement(275..658)
                     /gene="rplQ"
                     /locus_tag="K0W59_000136"
     CDS             complement(275..658)
                     /gene="rplQ"
                     /locus_tag="K0W59_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBI3021320.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            complement(699..1688)
                     /gene="rpoA"
                     /locus_tag="K0W59_000137"
     CDS             complement(699..1688)
                     /gene="rpoA"
                     /locus_tag="K0W59_000137"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBI3021321.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            complement(1714..2334)
                     /gene="rpsD"
                     /locus_tag="K0W59_000138"
     CDS             complement(1714..2334)
                     /gene="rpsD"
                     /locus_tag="K0W59_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBI3021322.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            complement(2368..2757)
                     /gene="rpsK"
                     /locus_tag="K0W59_000139"
     CDS             complement(2368..2757)
                     /gene="rpsK"
                     /locus_tag="K0W59_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBI3021323.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            complement(2774..3130)
                     /gene="rpsM"
                     /locus_tag="K0W59_000140"
     CDS             complement(2774..3130)
                     /gene="rpsM"
                     /locus_tag="K0W59_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBI3021324.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            complement(3277..3393)
                     /gene="rpmJ"
                     /locus_tag="K0W59_000141"
     CDS             complement(3277..3393)
                     /gene="rpmJ"
                     /locus_tag="K0W59_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBI3021325.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            complement(3425..4756)
                     /gene="secY"
                     /locus_tag="K0W59_000142"
     CDS             complement(3425..4756)
                     /gene="secY"
                     /locus_tag="K0W59_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBI3021326.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            complement(4764..5198)
                     /gene="rplO"
                     /locus_tag="K0W59_000143"
     CDS             complement(4764..5198)
                     /gene="rplO"
                     /locus_tag="K0W59_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBI3021327.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            complement(5202..5381)
                     /gene="rpmD"
                     /locus_tag="K0W59_000144"
     CDS             complement(5202..5381)
                     /gene="rpmD"
                     /locus_tag="K0W59_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBI3021328.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            complement(5385..5888)
                     /gene="rpsE"
                     /locus_tag="K0W59_000145"
     CDS             complement(5385..5888)
                     /gene="rpsE"
                     /locus_tag="K0W59_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBI3021329.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            complement(5903..6256)
                     /gene="rplR"
                     /locus_tag="K0W59_000146"
     CDS             complement(5903..6256)
                     /gene="rplR"
                     /locus_tag="K0W59_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBI3021330.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            complement(6266..6799)
                     /gene="rplF"
                     /locus_tag="K0W59_000147"
     CDS             complement(6266..6799)
                     /gene="rplF"
                     /locus_tag="K0W59_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBI3021331.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            complement(6813..7205)
                     /gene="rpsH"
                     /locus_tag="K0W59_000148"
     CDS             complement(6813..7205)
                     /gene="rpsH"
                     /locus_tag="K0W59_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBI3021332.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            complement(7239..7544)
                     /gene="rpsN"
                     /locus_tag="K0W59_000149"
     CDS             complement(7239..7544)
                     /gene="rpsN"
                     /locus_tag="K0W59_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBI3021333.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            complement(7559..8098)
                     /gene="rplE"
                     /locus_tag="K0W59_000150"
     CDS             complement(7559..8098)
                     /gene="rplE"
                     /locus_tag="K0W59_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBI3021334.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            complement(8113..8427)
                     /gene="rplX"
                     /locus_tag="K0W59_000151"
     CDS             complement(8113..8427)
                     /gene="rplX"
                     /locus_tag="K0W59_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBI3021335.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            complement(8438..8809)
                     /gene="rplN"
                     /locus_tag="K0W59_000152"
     CDS             complement(8438..8809)
                     /gene="rplN"
                     /locus_tag="K0W59_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBI3021336.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            complement(8974..9228)
                     /gene="rpsQ"
                     /locus_tag="K0W59_000153"
     CDS             complement(8974..9228)
                     /gene="rpsQ"
                     /locus_tag="K0W59_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBI3021337.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            complement(9228..9419)
                     /gene="rpmC"
                     /locus_tag="K0W59_000154"
     CDS             complement(9228..9419)
                     /gene="rpmC"
                     /locus_tag="K0W59_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBI3021338.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            complement(9419..9829)
                     /gene="rplP"
                     /locus_tag="K0W59_000155"
     CDS             complement(9419..9829)
                     /gene="rplP"
                     /locus_tag="K0W59_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBI3021339.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            complement(9842..10543)
                     /gene="rpsC"
                     /locus_tag="K0W59_000156"
     CDS             complement(9842..10543)
                     /gene="rpsC"
                     /locus_tag="K0W59_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBI3021340.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            complement(10561..10893)
                     /gene="rplV"
                     /locus_tag="K0W59_000157"
     CDS             complement(10561..10893)
                     /gene="rplV"
                     /locus_tag="K0W59_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBI3021341.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            complement(10908..11186)
                     /gene="rpsS"
                     /locus_tag="K0W59_000158"
     CDS             complement(10908..11186)
                     /gene="rpsS"
                     /locus_tag="K0W59_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBI3021342.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            complement(11203..12024)
                     /gene="rplB"
                     /locus_tag="K0W59_000159"
     CDS             complement(11203..12024)
                     /gene="rplB"
                     /locus_tag="K0W59_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBI3021343.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            complement(12042..12344)
                     /gene="rplW"
                     /locus_tag="K0W59_000160"
     CDS             complement(12042..12344)
                     /gene="rplW"
                     /locus_tag="K0W59_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBI3021344.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            complement(12341..12946)
                     /gene="rplD"
                     /locus_tag="K0W59_000161"
     CDS             complement(12341..12946)
                     /gene="rplD"
                     /locus_tag="K0W59_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBI3021345.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            complement(12957..13586)
                     /gene="rplC"
                     /locus_tag="K0W59_000162"
     CDS             complement(12957..13586)
                     /gene="rplC"
                     /locus_tag="K0W59_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBI3021346.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            complement(13619..13930)
                     /gene="rpsJ"
                     /locus_tag="K0W59_000163"
     CDS             complement(13619..13930)
                     /gene="rpsJ"
                     /locus_tag="K0W59_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBI3021347.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            14309..14776
                     /locus_tag="K0W59_000164"
     CDS             14309..14776
                     /locus_tag="K0W59_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBI3021348.1"
                     /translation="MDTTLPFLILYACLSDLLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            complement(14773..15249)
                     /gene="bfr"
                     /locus_tag="K0W59_000165"
     CDS             complement(14773..15249)
                     /gene="bfr"
                     /locus_tag="K0W59_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HBI3021349.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(15322..15516)
                     /gene="bfd"
                     /locus_tag="K0W59_000166"
     CDS             complement(15322..15516)
                     /gene="bfd"
                     /locus_tag="K0W59_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HBI3021350.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            complement(15699..16883)
                     /gene="tuf"
                     /locus_tag="K0W59_000167"
     CDS             complement(15699..16883)
                     /gene="tuf"
                     /locus_tag="K0W59_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBI3021351.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVG
                     ILLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFR
                     TTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKV
                     LG"
     gene            complement(16954..19068)
                     /gene="fusA"
                     /locus_tag="K0W59_000168"
     CDS             complement(16954..19068)
                     /gene="fusA"
                     /locus_tag="K0W59_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBI3021352.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(19165..19635)
                     /gene="rpsG"
                     /locus_tag="K0W59_000169"
     CDS             complement(19165..19635)
                     /gene="rpsG"
                     /locus_tag="K0W59_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBI3021353.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(19732..20106)
                     /gene="rpsL"
                     /locus_tag="K0W59_000170"
     CDS             complement(19732..20106)
                     /gene="rpsL"
                     /locus_tag="K0W59_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBI3021354.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(20232..20519)
                     /gene="tusB"
                     /locus_tag="K0W59_000171"
     CDS             complement(20232..20519)
                     /gene="tusB"
                     /locus_tag="K0W59_000171"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HBI3021355.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(20527..20886)
                     /gene="tusC"
                     /locus_tag="K0W59_000172"
     CDS             complement(20527..20886)
                     /gene="tusC"
                     /locus_tag="K0W59_000172"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HBI3021356.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(20886..21272)
                     /gene="tusD"
                     /locus_tag="K0W59_000173"
     CDS             complement(20886..21272)
                     /gene="tusD"
                     /locus_tag="K0W59_000173"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HBI3021357.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(21272..21994)
                     /locus_tag="K0W59_000174"
     CDS             complement(21272..21994)
                     /locus_tag="K0W59_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI3021358.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(22161..22973)
                     /gene="fkpA"
                     /locus_tag="K0W59_000175"
     CDS             complement(22161..22973)
                     /gene="fkpA"
                     /locus_tag="K0W59_000175"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBI3021359.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            23194..23412
                     /locus_tag="K0W59_000176"
     CDS             23194..23412
                     /locus_tag="K0W59_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HBI3021360.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(23461..24051)
                     /gene="slyD"
                     /locus_tag="K0W59_000177"
     CDS             complement(23461..24051)
                     /gene="slyD"
                     /locus_tag="K0W59_000177"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBI3021361.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(24146..24346)
                     /locus_tag="K0W59_000178"
     CDS             complement(24146..24346)
                     /locus_tag="K0W59_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HBI3021362.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(24356..26161)
                     /gene="kefB"
                     /locus_tag="K0W59_000179"
     CDS             complement(24356..26161)
                     /gene="kefB"
                     /locus_tag="K0W59_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HBI3021363.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGVAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARVRGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(26161..26712)
                     /gene="kefG"
                     /locus_tag="K0W59_000180"
     CDS             complement(26161..26712)
                     /gene="kefG"
                     /locus_tag="K0W59_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HBI3021364.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSSIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            26843..28756
                     /locus_tag="K0W59_000181"
     CDS             26843..28756
                     /locus_tag="K0W59_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI3021365.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIQ
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQAVTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRQEAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            28756..29778
                     /locus_tag="K0W59_000182"
     CDS             28756..29778
                     /locus_tag="K0W59_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBI3021366.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYLLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            29772..29990
                     /locus_tag="K0W59_000183"
     CDS             29772..29990
                     /locus_tag="K0W59_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HBI3021367.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            30044..30913
                     /locus_tag="K0W59_000184"
     CDS             30044..30913
                     /locus_tag="K0W59_000184"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HBI3021368.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     LLVQRLMEGKKIE"
     gene            complement(30968..31372)
                     /locus_tag="K0W59_000185"
     CDS             complement(30968..31372)
                     /locus_tag="K0W59_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HBI3021369.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            31674..32306
                     /gene="crp"
                     /locus_tag="K0W59_000186"
     CDS             31674..32306
                     /gene="crp"
                     /locus_tag="K0W59_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HBI3021370.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            32357..34447
                     /locus_tag="K0W59_000187"
     CDS             32357..34447
                     /locus_tag="K0W59_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBI3021371.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(34514..35734)
                     /gene="argD/dapC"
                     /locus_tag="K0W59_000188"
     CDS             complement(34514..35734)
                     /gene="argD/dapC"
                     /locus_tag="K0W59_000188"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HBI3021372.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPQPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQVLLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(35820..36383)
                     /gene="pabA"
                     /locus_tag="K0W59_000189"
     CDS             complement(35820..36383)
                     /gene="pabA"
                     /locus_tag="K0W59_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HBI3021373.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(36415..37017)
                     /locus_tag="K0W59_000190"
     CDS             complement(36415..37017)
                     /locus_tag="K0W59_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HBI3021374.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEIDVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(37007..37174)
                     /locus_tag="K0W59_000191"
     CDS             complement(37007..37174)
                     /locus_tag="K0W59_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HBI3021375.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(37279..37851)
                     /gene="ppiA"
                     /locus_tag="K0W59_000192"
                     /gene_synonym="rotA"
     CDS             complement(37279..37851)
                     /gene="ppiA"
                     /locus_tag="K0W59_000192"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HBI3021376.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            38122..39303
                     /gene="tsgA"
                     /locus_tag="K0W59_000193"
     CDS             38122..39303
                     /gene="tsgA"
                     /locus_tag="K0W59_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HBI3021377.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFSCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            39565..42108
                     /gene="nirB"
                     /locus_tag="K0W59_000194"
     CDS             39565..42108
                     /gene="nirB"
                     /locus_tag="K0W59_000194"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HBI3021378.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHSSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIARKFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            42105..42431
                     /gene="nirD"
                     /locus_tag="K0W59_000195"
     CDS             42105..42431
                     /gene="nirD"
                     /locus_tag="K0W59_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HBI3021379.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            42557..43363
                     /gene="nirC"
                     /locus_tag="K0W59_000196"
     CDS             42557..43363
                     /gene="nirC"
                     /locus_tag="K0W59_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HBI3021380.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWIALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            43382..44755
                     /gene="cobA"
                     /locus_tag="K0W59_000197"
     CDS             43382..44755
                     /gene="cobA"
                     /locus_tag="K0W59_000197"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBI3021381.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            45002..45169
                     /locus_tag="K0W59_000198"
     CDS             45002..45169
                     /locus_tag="K0W59_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HBI3021382.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            45464..46801
                     /gene="frlA"
                     /locus_tag="K0W59_000199"
                     /pseudo
     CDS             45464..46801
                     /gene="frlA"
                     /locus_tag="K0W59_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            46822..47844
                     /gene="frlB"
                     /locus_tag="K0W59_000200"
     CDS             46822..47844
                     /gene="frlB"
                     /locus_tag="K0W59_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HBI3021383.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            47894..48723
                     /gene="frlC"
                     /locus_tag="K0W59_000201"
                     /pseudo
     CDS             47894..48723
                     /gene="frlC"
                     /locus_tag="K0W59_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            48720..49505
                     /gene="frlD"
                     /locus_tag="K0W59_000202"
     CDS             48720..49505
                     /gene="frlD"
                     /locus_tag="K0W59_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HBI3021384.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            49605..50336
                     /gene="phoB"
                     /locus_tag="K0W59_000203"
     CDS             49605..50336
                     /gene="phoB"
                     /locus_tag="K0W59_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HBI3021385.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(50496..51581)
                     /locus_tag="K0W59_000204"
     CDS             complement(50496..51581)
                     /locus_tag="K0W59_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBI3021386.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLLALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(51593..52897)
                     /locus_tag="K0W59_000205"
     CDS             complement(51593..52897)
                     /locus_tag="K0W59_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HBI3021387.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTWLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(52909..53262)
                     /locus_tag="K0W59_000206"
     CDS             complement(52909..53262)
                     /locus_tag="K0W59_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HBI3021388.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EYVEHAIPMLINHLK"
     gene            complement(53273..54151)
                     /locus_tag="K0W59_000207"
     CDS             complement(53273..54151)
                     /locus_tag="K0W59_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HBI3021389.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELV
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLCQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(54148..55374)
                     /locus_tag="K0W59_000208"
     CDS             complement(54148..55374)
                     /locus_tag="K0W59_000208"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBI3021390.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(55375..56538)
                     /locus_tag="K0W59_000209"
     CDS             complement(55375..56538)
                     /locus_tag="K0W59_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HBI3021391.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLKA"
     gene            complement(56622..56984)
                     /locus_tag="K0W59_000210"
     CDS             complement(56622..56984)
                     /locus_tag="K0W59_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBI3021392.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(57001..57906)
                     /locus_tag="K0W59_000211"
     CDS             complement(57001..57906)
                     /locus_tag="K0W59_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3021393.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLLATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(58176..59180)
                     /gene="trpS"
                     /locus_tag="K0W59_000212"
     CDS             complement(58176..59180)
                     /gene="trpS"
                     /locus_tag="K0W59_000212"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBI3021394.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(59173..59931)
                     /gene="gph"
                     /locus_tag="K0W59_000213"
     CDS             complement(59173..59931)
                     /gene="gph"
                     /locus_tag="K0W59_000213"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HBI3021395.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(59924..60601)
                     /gene="rpe"
                     /locus_tag="K0W59_000214"
     CDS             complement(59924..60601)
                     /gene="rpe"
                     /locus_tag="K0W59_000214"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBI3021396.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(60619..61455)
                     /gene="dam"
                     /locus_tag="K0W59_000215"
     CDS             complement(60619..61455)
                     /gene="dam"
                     /locus_tag="K0W59_000215"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBI3021397.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(61562..62848)
                     /gene="damX"
                     /locus_tag="K0W59_000216"
     CDS             complement(61562..62848)
                     /gene="damX"
                     /locus_tag="K0W59_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HBI3021398.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(62940..64028)
                     /gene="aroB"
                     /locus_tag="K0W59_000217"
     CDS             complement(62940..64028)
                     /gene="aroB"
                     /locus_tag="K0W59_000217"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBI3021399.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(64085..64606)
                     /gene="aroK"
                     /locus_tag="K0W59_000218"
     CDS             complement(64085..64606)
                     /gene="aroK"
                     /locus_tag="K0W59_000218"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HBI3021400.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQKLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(65007..66245)
                     /gene="hofQ"
                     /locus_tag="K0W59_000219"
     CDS             complement(65007..66245)
                     /gene="hofQ"
                     /locus_tag="K0W59_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HBI3021401.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQASSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(66157..66561)
                     /gene="hofP"
                     /locus_tag="K0W59_000220"
     CDS             complement(66157..66561)
                     /gene="hofP"
                     /locus_tag="K0W59_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HBI3021402.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(66551..66991)
                     /gene="hofO"
                     /locus_tag="K0W59_000221"
     CDS             complement(66551..66991)
                     /gene="hofO"
                     /locus_tag="K0W59_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HBI3021403.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(66975..67514)
                     /gene="hofN"
                     /locus_tag="K0W59_000222"
     CDS             complement(66975..67514)
                     /gene="hofN"
                     /locus_tag="K0W59_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HBI3021404.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(67514..68293)
                     /gene="hofM"
                     /locus_tag="K0W59_000223"
     CDS             complement(67514..68293)
                     /gene="hofM"
                     /locus_tag="K0W59_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HBI3021405.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            68413..70965
                     /gene="mrcA"
                     /locus_tag="K0W59_000224"
     CDS             68413..70965
                     /gene="mrcA"
                     /locus_tag="K0W59_000224"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HBI3021406.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(71130..71690)
                     /gene="nudE"
                     /locus_tag="K0W59_000225"
     CDS             complement(71130..71690)
                     /gene="nudE"
                     /locus_tag="K0W59_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HBI3021407.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            72010..74145
                     /gene="igaA"
                     /locus_tag="K0W59_000226"
     CDS             72010..74145
                     /gene="igaA"
                     /locus_tag="K0W59_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HBI3021408.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRQRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLVLNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            74210..74878
                     /gene="yrfG"
                     /locus_tag="K0W59_000227"
     CDS             74210..74878
                     /gene="yrfG"
                     /locus_tag="K0W59_000227"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HBI3021409.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            74889..75290
                     /gene="hslR"
                     /locus_tag="K0W59_000228"
     CDS             74889..75290
                     /gene="hslR"
                     /locus_tag="K0W59_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HBI3021410.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIKGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            75315..76193
                     /gene="hslO"
                     /locus_tag="K0W59_000229"
     CDS             75315..76193
                     /gene="hslO"
                     /locus_tag="K0W59_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBI3021411.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(76328..78052)
                     /locus_tag="K0W59_000230"
     CDS             complement(76328..78052)
                     /locus_tag="K0W59_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HBI3021412.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            78431..80053
                     /gene="pckA"
                     /locus_tag="K0W59_000231"
     CDS             78431..80053
                     /gene="pckA"
                     /locus_tag="K0W59_000231"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBI3021413.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(80129..81481)
                     /gene="envZ"
                     /locus_tag="K0W59_000232"
     CDS             complement(80129..81481)
                     /gene="envZ"
                     /locus_tag="K0W59_000232"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HBI3021414.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(81478..82197)
                     /gene="ompR"
                     /locus_tag="K0W59_000233"
     CDS             complement(81478..82197)
                     /gene="ompR"
                     /locus_tag="K0W59_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HBI3021415.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            82425..82901
                     /gene="greB"
                     /locus_tag="K0W59_000234"
     CDS             82425..82901
                     /gene="greB"
                     /locus_tag="K0W59_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HBI3021416.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            82998..85319
                     /locus_tag="K0W59_000235"
     CDS             82998..85319
                     /locus_tag="K0W59_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBI3021417.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQYPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNGVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            85757..85984
                     /gene="feoA"
                     /locus_tag="K0W59_000236"
     CDS             85757..85984
                     /gene="feoA"
                     /locus_tag="K0W59_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HBI3021418.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            86001..88322
                     /gene="feoB"
                     /locus_tag="K0W59_000237"
     CDS             86001..88322
                     /gene="feoB"
                     /locus_tag="K0W59_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HBI3021419.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            88322..88558
                     /gene="feoC"
                     /locus_tag="K0W59_000238"
     CDS             88322..88558
                     /gene="feoC"
                     /locus_tag="K0W59_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HBI3021420.1"
                     /translation="MASLIQVRDLLALRCRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            88761..89639
                     /gene="rpnA"
                     /locus_tag="K0W59_000239"
     CDS             88761..89639
                     /gene="rpnA"
                     /locus_tag="K0W59_000239"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HBI3021421.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            89712..89981
                     /locus_tag="K0W59_000240"
     CDS             89712..89981
                     /locus_tag="K0W59_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBI3021422.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            89975..90340
                     /locus_tag="K0W59_000241"
     CDS             89975..90340
                     /locus_tag="K0W59_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBI3021423.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPCDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(90341..91129)
                     /gene="bioH"
                     /locus_tag="K0W59_000242"
     CDS             complement(90341..91129)
                     /gene="bioH"
                     /locus_tag="K0W59_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HBI3021424.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPYFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            91167..91850
                     /gene="gntX"
                     /locus_tag="K0W59_000243"
     CDS             91167..91850
                     /gene="gntX"
                     /locus_tag="K0W59_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HBI3021425.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            91909..92484
                     /gene="nfuA"
                     /locus_tag="K0W59_000244"
     CDS             91909..92484
                     /gene="nfuA"
                     /locus_tag="K0W59_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HBI3021426.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            92845..94149
                     /gene="gntT"
                     /locus_tag="K0W59_000245"
     CDS             92845..94149
                     /gene="gntT"
                     /locus_tag="K0W59_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBI3021427.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLANCGGAQRIATTLIAKFGKKHIQWAVVLTGF
                     TVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGPTAIA
                     TIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEEMPSF
                     GVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLN
                     RGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNISPLL
                     MAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDP
                     GFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(94194..96278)
                     /gene="malQ"
                     /locus_tag="K0W59_000246"
     CDS             complement(94194..96278)
                     /gene="malQ"
                     /locus_tag="K0W59_000246"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HBI3021428.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(96288..98681)
                     /gene="malP"
                     /locus_tag="K0W59_000247"
     CDS             complement(96288..98681)
                     /gene="malP"
                     /locus_tag="K0W59_000247"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HBI3021429.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
BASE COUNT        23778 a        26823 c        24730 g        23750 t
ORIGIN      
        1 ctgagcgatg gcccggatgg tcagtttatc ttcaaatctt aaatgcaggg catcaggcaa
       61 ataagaacgg aagcagggaa tatcttttgt tgtctgggaa ttcatcgttc gtgtccatca
      121 atatagatgg gcgcgattgt tgccagacag gacaattttc acaagacgtc gctgatgggg
      181 cgcttaccaa aaagagtaca gctattgtag ataagtgggg atactacggg tataaaaaaa
      241 cccgccgggg cgggtttttt tacgttgctt cagattactc tgcagcagct tctgctttct
      301 ctgaacgatc aaccagctcg atgtaagcca tcggcgcgtt gtcgcctgca cggaagccac
      361 acttcagaat acgagtgtaa ccaccggcac ggctcgcgaa acgcgggccc agttcgttaa
      421 acagttttgc cacgatctcg ttatcacgag tacgggcgaa tgccagacga cgattagcaa
      481 cgctatcagt cttggcaaga gtaatcagcg gctcaactac gcggcgcagc tctttcgctt
      541 taggcagagt cgtcttgatg atttcatgac gaaccagtga acctgccata ttgcggaaca
      601 tagcctggcg atggctgctg ttgcggttca gttgacgacc actcttacga tggcgcatga
      661 ccttatcctt ctcagtaaaa ccttaacctg tgatccggtt actcgtcagc gatgcttgcc
      721 ggtggccagt tttccaagcg catgcccaga gacagtccac gggaagccag cacgtcttta
      781 atctcagtaa gagatttttt accaaggtta ggcgttttaa ggagctcaac ctcggtacgc
      841 tgtaccagat caccgatata gtggatagct tctgctttaa ggcagttagc agagcggaca
      901 gtcaattcca gatcgtcaac agggcgcagc aggatcggat cgaactctgg tttctcttct
      961 ttcacttcag gctgacgtac atcacgtaag tcaacgaaag cttccagttg ttcagccaga
     1021 atggttgccg cacgacgaat cgcctcttca ggatcgattg tgccgttggt ttccatttcg
     1081 atgaccagct tgtccaggtc ggtacgctgt tctacacgcg ctgcttcaac attgtaggca
     1141 atacgctcca cagggctgta gcatgcgtcg accagcagac ggccgattgg gcgctcatct
     1201 tcttccgaat gaattcgggt agaagccggc acataaccac gaccgcgctg aactttgata
     1261 cgcatgctaa tagacgcgtt ctcatcggtc aggtggcaga tcacgtgctg cggcttgacg
     1321 atttcgacat caccgtcgtg ggtgatatcg gctgcagtca cagggccaat gccagattta
     1381 ttcaaggtaa gaataacttc atctttgccc tgaactctca ccgccagccc tttcaggttg
     1441 agcaggattt ccaggatatc ttcctgaacg ccttctttgg tgctgtactc atgtagtaca
     1501 ccatcaatct caacctcggt caccgcgcaa cccggcatcg atgagagcag aatacggcgc
     1561 agtgcgttac ccagagtatg gccaaagcca cgctctaaag gctcaagggt caccttggcg
     1621 tgcgtcgaac tcacttgctc gatatcaacc aggcgcggtt ttagaaactc tgtcacagaa
     1681 ccctgcattg tgtcctctct ttggtactaa gctttacttg gagtaaagct cgacgatcag
     1741 gtgttcgtta atgtccgcag acagatcaga acgctccggc ttacgcttaa acgtaccttc
     1801 catcttacca gcatcaactt ccagccaagt tggcttttca cgctgctcag ccagctccag
     1861 agcggctttc acgcgagact gcttcttcgc tttctcacga atgcttacaa cgtcattcgg
     1921 actaacctga taagaagcga tgttaacaac acgaccgttt accataattg ctttatggct
     1981 aaccagctga cgtgcttctg cacgagtggc accgaagccc atacggtata caacgttgtc
     2041 cagacgacct tccagcagag ccaacaggtt ttcaccggtg ttgcctttca gacgtgctgc
     2101 ttctttgtag tagttacgga actgacgctc cagcacacca tagatacggc gaactttttg
     2161 cttttcacgc aactgcacac catagtcaga cagacgcggt ttacgcgcac cgtgctggcc
     2221 aggagcttgt tcaattttac acttggtatc gatcgcgcga acgccagact taaggaataa
     2281 gtcggtgccc tcacgacggc tcagcttgag cttaggaccc aaatatcttg ccattttctt
     2341 tctccaacaa acctggaaaa cgaggcgtta tacgcgacgt tttttcggcg gacgacaacc
     2401 gttatgaggg atcggagtca catcagtaat gttagtgatg cggaaacctg cggcgttcag
     2461 agcacgaata gtagattcgc ggcctggacc cggaccttta accataactt ccagattctt
     2521 gatgccgtat tctttcacgg cgtcagcgca acgctctgct gcaacctgag ctgcaaacgg
     2581 agtggatttg cgagaaccac ggaaaccgga accaccggct gttgcccaac ccaacgcgtt
     2641 accctgacga tcagtgatag tcacgatggt gttgttgaaa gaagcatgga tatgagccac
     2701 gccgtcagag acttgttttc ttacacgttt acgtgcacga attggtgcct ttgccattat
     2761 tcaatcaccc cgattatttc ttgatcggtt tgcgcggacc cttacgggta cgtgcgttgg
     2821 tcttggtacg ctgaccgcga accgggagac cacgacgatg acgcaaaccg cgatagcaac
     2881 caagatccat caggcgcttg atgctcatgc tgatttcacg gcgcagatca ccttcaacga
     2941 caaatttggc aacttcgtca cgcagcgtgt cgatttgtcc ttcagacagc tcactgatct
     3001 taacatcttc agcgataccc gctgcagcca ggatggcttt agaacgggtc ttgccgacgc
     3061 cataaatcga agttaatgcg attacggcat gcttatgatc aggaatgtta atgcctgcta
     3121 tacgggccac tatgcactcc tactatttaa tatgtacgtt ccatgctgaa aagcccgttt
     3181 tcaggatact caaatggaaa cgcacagaca tacaaaagat tggctggcta atctagccag
     3241 ctcaacccaa ctttgcaaga aaaatatgcg aaaaaatcag ccttggcgct gtttatgctt
     3301 cggctcggca ctgcaaatca cacggatgac accatcacgc ttaacgattt tgcagttacg
     3361 gcataatttc ttgacggaag cacgaacttt catttttact ctccgtaact tctcgggcga
     3421 ccaattatcg gccgtagcct ttcaggttcg ccttcttcaa tgcagactca tactgactgg
     3481 acatcatcag agtttgcact tgagccataa agtccataat cacgacaaca acgataagca
     3541 gtgaggtccc accgaagtag aacggtactt tcattgcatc acgcatgaac tccgggatca
     3601 ggcagataaa ggtaatatac agcgcaccaa ccaaggtcag gcgggtcatt actttatcga
     3661 tatacttcgc cgtttgctct cccggacgaa ttcctggtac aaatgcaccg gacttcttca
     3721 ggttatctgc tgtttcacgc gggttgaaaa ccaacgccgt gtagaagaaa cagaagaaga
     3781 tgattgcaga cgcatagagt aacacataaa gcggttgccc aggctgcaaa tacagcgaaa
     3841 ttgttgtcag ccagttccaa ccagtaccgc ccccgaacca tgacgcgatg gtcgccggga
     3901 acagaataat actggaagcg aagattgccg ggattacccc cgccatattc actttcagcg
     3961 gtaaatgtgt gctctgtgca gcatagacac gacgaccttg ctgacgtttc gcgtagttta
     4021 ccacaatgcg gcgttgacca cgctcaacaa acacaacaaa gaacgtcact gcaaatacta
     4081 atactgcaac caacagcaac acgaggaagt gcaggtcgcc ttgacgcgct tgctcgatag
     4141 tatgggcaat ggctggcggg agtcccgcga caataccggc gaagataatg attgaaatac
     4201 cgttgccgat acctcgttca gtgatctgtt cgcccaacca catcaggaac atcgttcctg
     4261 tgaccagact tacaacagcg gtgaagtaga atgcaaagcc cgggttaatc accaggcctt
     4321 gcataccagg catattcggc agaccggtag caataccgat cgactggaat attgccagca
     4381 ccagagtacc gtaacgggtg tactggctga tcttacgacg accagactcc ccttctttct
     4441 taatttctgc caacgttggg tgaaccaccg tcagcagctg gataatgatc gacgccgaaa
     4501 tatacggcat gatccccaga gcaaagatag aagcacggct gagagcacca ccagagaaca
     4561 tgttaaacat ctcaatgatg gtgcctcgct gttgctcaag cagtttggca agtacagcgg
     4621 catcaatacc agggatcgga ataaaagagc caatacggaa cacaatcagc gcaccgataa
     4681 caaacagcag tctgcgtttc agctcgccta agccaccttt ggcactttga aaatctaatc
     4741 ccggttgttt agccatctgc tacttattcc tcgattttac cgccagcagc ttcgatagca
     4801 gcacgagcgc ctttagtaac acgcaggcca cgaacagtta ccggagtcgt tacttcgcca
     4861 gccaggatca ctttcgcgaa ctcgatctgg ataccgataa tgttagccgc tttcagcgtg
     4921 ttcaggtcta caacaccgcc ttctacttta gccaggtcag atagacgaac ttcggctgta
     4981 atcgctgctt tacgagaagt gaagccgaat ttcggcagac gacggtacag aggcatctga
     5041 ccaccctcga aaccgcgacg tacgccaccg ccagaacgag acttctgacc tttgtgacca
     5101 cgaccaccgg ttttaccgag gccagaaccg ataccacgcc ccaggcgttt acccgccttt
     5161 ttggagcctt cggccggaga cagagtattt aaacgcatct cttactcctc aactttaacc
     5221 atgaaggaaa ccgcgttgat cataccgcga atagcaggag tatcctcgcg ctctacggtg
     5281 tgaccaatac gacgcagacc caagccaagc agcgttgcct tgtgtttcgg cagacgaccg
     5341 attgcactgc gggtttgagt aattttaata gtctttgcca tggtttattt ccccagaatt
     5401 tcttcaacgg atttaccacg cttggcagcg accatttctg gagaattcat attttctagg
     5461 ccatcaatag ttgcacgaac cacgttgatc gggttggtgg aaccatatgc tttagccaga
     5521 acgttatgaa ccccagcgac ttccagaacg gcgcgcattg caccaccggc gatgataccg
     5581 gtaccttcgg aagccggctg catgaatacg cgagaacccg tgtgaacacc tttaacaggg
     5641 tgttgtagag tgccgttatt cagcgcgacg ttaatcatat tgcgacgggc tttttccatc
     5701 gctttctgga tcgctgctgg aacttcacgc gctttaccgt aaccaaaacc aacgcgaccg
     5761 ttaccatcgc caactacagt cagagctgtg aaggagaaaa tacgaccacc tttaacggtt
     5821 ttagatacgc ggtttaccgc gatcagcttt tcctgcagtt cgccagcttg tttttcgatg
     5881 tgagccatct tacacctcta ccttagaact gaaggccagc ttcacgggca gcatctgcca
     5941 gtgcctggac acgaccatga tattggaacc cggaacggtc aaaggataca tctttgatgc
     6001 ctttttccag agcgcgttca gcgacagctt tacccacagc tgcagccgcg tctttgttac
     6061 cggtgtactt cagttgttca gcgatagctt tttctacagt agaagcagct accagaactt
     6121 cagaaccgtt cggtgcaatt acctgtgcgt aaatgtgacg cggggtacga tgtaccacca
     6181 ggcgagttgc gcccagctcc tggagcttgc ggcgtgcgcg ggtcgcacga cggatacgag
     6241 cagatttctt atccatagtg ttaccttact tcttcttagc ctctttggta cgcacgactt
     6301 cgtcggcgta acgaacaccc ttgcctttgt aaggctcagg acgacggtag gcgcgcagat
     6361 ccgctgcaac ctggccgatc acctgcttat cagcgccttt cagcacgatt tcagtctgag
     6421 tcggacattc agcagtgata cccgcaggca gctgatggtc aacaggatga gagaaaccca
     6481 gagacaggtt aatcacattg cctttaaccg ctgcacggta acctacacca accagctgca
     6541 gcttcttagt gaagccttcg gtaacaccga taaccattga gttcagcagg gcacgcgcgg
     6601 taccagcctg tgcccaaccg tctgcgtaac catcacgcgg accgaaggtc agggtattat
     6661 ctgcatgttt aacttcaaca gcatcgttga gagtacgagt cagctcgccg tttttacctt
     6721 tgatcgtaat aacctgaccg ttgattttta cgtcaacgcc ggcaggaaca acgaccggtg
     6781 ctttagcaac acgagacatt ttttcctccg atttaggcta cgtagcagat aatttcgcca
     6841 ccaagaccag cctggcgcgc tgcacgatca gtcataacac ctttagaggt agaaacaact
     6901 gcgataccca gacccgccat aactttcggc agctcatctt tacgtttata gatgcgcaga
     6961 cctgggcggc tgacacgctg aatgctttct acaacagctt tgccctggaa atacttcaga
     7021 gtaagttcca gttcaggctt ggtgtcgcct tcaactttaa aatcttcaat aaaaccttct
     7081 tccttcagca cgttggcgat tgccactttc agcttggagg aaggcatggt gaccgcagct
     7141 ttgttcgcgg cctgaccgtt acggatacgg gtcagcatat ccgcgatcgg atcttgcatg
     7201 ctcatctgtc tttactcccg tgattcaatt ggtgacaatt accagctagc ctttttcaga
     7261 cccgggattt caccgcgcat agcggcttca cggaccttaa tacggctcaa cccgaacttc
     7321 cgcaggaaac catgcggacg acctgtttga cggcagcggt tacgctgacg agacgggctg
     7381 gaatcacgcg gcagagtctg cagcttgaga acagcgttcc aacgatcttc gtcggaagcg
     7441 ttcacatcag agatgatcgc tttcagttca gcgcgtttcg cgaagtattt atcagctaaa
     7501 gctacgcgtt ttacttcgcg tgctttcatt gattgcttag ccatttagta accctacctt
     7561 acttgcggaa cgggaagtca aaggcagcca gcagagcgcg gccttcttcg tcagatttcg
     7621 cagtagtggt aatggtaata tccaaaccac gaacgcggtc gactttatcg tagtcgattt
     7681 ctgggaagat gatctgctca cggacaccca tgctgtagtt accacgaccg tcgaaagact
     7741 tagcggacag gccacggaag tcacggatac gaggtacagc aatagtgatc aggcgctcaa
     7801 agaactccca catgcgttcg ccacgcagag ttactttaca gccgatcgga tagccctgac
     7861 ggattttgaa gcctgcaaca gatttgcgtg ctttggtgat cagcggtttt tgaccggaga
     7921 ttgctgccag gtctgctgct gcgttatcca gcagtttttt gtcagcgatc gcttcaccaa
     7981 cacccatgtt cagggtgatc ttctcgaccc gagggacttg catgacagaa ttgtagttaa
     8041 actcagtcat gagtttttta actacttcgt ctttgtagta atcatgcagt ttcgccatcg
     8101 tactactcca aattacttga tagtttcgct gttagacttg aagaaacgga cttttttgcc
     8161 gtcttcgaat ctaaagccta cacggtcagc cttgccggtt gccgcattga agattgctac
     8221 gttggaaacc tgaatagcgg cttctttttc aacgatgcca cccggttggt tcagggccgg
     8281 aaccggcttc tgatgtttct taaccaggtt gataccttca acaatgacct tgccggaaga
     8341 caggacattc ttaactttac cgcgtttacc tttatcttta ccggttaaca cgataacttc
     8401 gtcatcacga cggattttcg ctgccatgat tcgctcctta gagtacttct ggtgccagag
     8461 agataatttt catgaacttc tcactacgaa gctcacgagt taccggccca aaaatacgcg
     8521 taccgatagg ctgctcgctg ttgttgttca gaagaacaca agcattacca tcgaagcgaa
     8581 tgacagaacc gtccgggcga cgaacaccct tcttggtgcg caccactacc gccttcagca
     8641 catcaccttt tttgacctta ccacgcggaa ttgcttcttt gatggtgatc ttgatgatgt
     8701 cgcctacgcc tgcgtagcga cggtgcgagc cacccagaac cttgatacac attacgcgac
     8761 gtgcaccgga gttgtcggcg acgttcagca tagtctgttc ttggatcatt ttagtgctcc
     8821 gctaatgtca actactactg agacccgaaa atcaggtcgt taaaaatccc catatcgagg
     8881 gcgcggcatt ataacaccgc ttcaaggata tgggtagaaa aaataaacgg ctcatttctg
     8941 agccgtttat tcgtatcgag agagtgtact gtattacaga accgctttct ctacaacgcg
     9001 aaccagcgtc caggatttag tcttggacag cggacggcat tcgcggattt caaccacgtc
     9061 accgataccg cattcgttgt tctcgtcatg tacgtgcagt ttggtcgtac gcttgatgaa
     9121 tttaccgtag atcgggtgtt tcacaaaacg ttcgatagca acaacaatgg atttctccat
     9181 tttgtcgcta acaacgcgac cttgcagagt acggatttta tcggtcatta cgcacccgcc
     9241 ttctcgttca gtaaagtctt aacgcgtgcg acatcgcgac gcacttgctt caacaggtga
     9301 gactgttgca gctggccact tgcagcctgc atacgcaggt tgaactgctc acgcagcagg
     9361 ttcagcagct cggtgttcag ctcttcaatg ctcttctcac gcagctcttt tgctttcatt
     9421 acatcaccgt cttagttaca aaggtggttt taatcggcag tttcgctgct gccagcttga
     9481 atgcttcacg ggccagctct tccggaacac cgtccatttc atacaggact ttacccggct
     9541 gaatcaaggc aacccaatac tccacgttac ctttaccttt acccatacgc actgccagcg
     9601 gcttttcagt gatcggtttg tccgggaaca cacggatcca gatcttacct tgacgcttaa
     9661 ctgcacgggt catagcacga cgtgctgctt cgatctgacg ggcagtcaga cgaccacggc
     9721 caacagcttt cagaccgaag ctgccgaagc taacatccgt accctgcgcc agaccgcggt
     9781 tacggccttt gtgcatttta cggaattttg tacgctttgg ttgtaacatc agcgacgctc
     9841 cttatttacg gcctttacgc tgctgctttt taggctgagc agccggtttt tccggttgtt
     9901 caacagcagc cataccaccc aggatctcgc ctttgaagat ccacacttta acgccgatta
     9961 caccgtaagt ggtgtgcgct tcagaggtgt tgtagtcgat gtcagcacgc agagtgtgca
    10021 gcggtacgcg accttcgcgg taccattcgg tacgtgcgat ttccgcgccg cccagacggc
    10081 cgctaacttc aactttaata cctttagcgc ccagacgcat tgcgttctgt acagcacgct
    10141 tcatagcacg acggaacata acgcgacgtt ccagctgaga agtgatgctg tcagcaacca
    10201 gttttgcgtc cagttcaggc ttacgaactt cggcgatgtt gatctgtgca ggaacgccag
    10261 cgatgtccgc tacgacctta cgcagttttt ctacgtcttc acctttctta ccgataacga
    10321 tacccgggcg agcagtgtga atggttacac ggatgctctt agccggacgc tcgataacga
    10381 tacgagatac ggacgcttta gccagttcct tagtcaggta ctgacgtact ttaaaatcgc
    10441 tgtccaggtt gtcagcgaat tctttggtgt tcgcaaacca ggtagagttc catggtttta
    10501 caatacccag gcgaatacca ttaggatgta ctttctgacc cattgctagt ctccagagtc
    10561 tcagcgatcg gacacaacca cagtgatgtg gctggtgcgc ttcaggatac gatctgcacg
    10621 accttttgca cgcggcataa tgcgcttcat gctcgggcct tcgtctacga aaattttcgt
    10681 aactttcaga tcgtcaatgt cagcgccatc gttgtgttca gcgttagcaa tggcagattc
    10741 cagaactttc ttgacctgta cagccgcttt cttgttggtg taggtcaaaa tatccagagc
    10801 ctgcgacact ttcttaccgc gaatcaggtc agcaacaagg cgaaccttct gagcagaaga
    10861 acgagcatgg cgatgtttag caatagtttc catctcttcc tcctacctta tttcttcttc
    10921 gcttttttat cagcagcgtg gccgcgataa gtacgagtcg gtgcgaattc acccagtttg
    10981 tgaccgacca tttcgtcggt tacaaatacc ggaacgtgct gacgaccatt atggacagcg
    11041 atggtcaaac cgatcatgtt aggaaagatc gttgaacgac gggaccaagt gcgcaggggc
    11101 ttcttgtctc cgctttccac cgctttctct accttcttca gcaagtgcag gtcaataaaa
    11161 ggacctttct tgagagaacg tggcatggct tatcctctaa aattatttgc tacggcgacg
    11221 tacgatgaat ttatcagtac gcttgttgct gcgggtcttc ttacctttgg tctgaacgcc
    11281 ccacggagtt accgggtgct taccaaagtt acgaccttca ccaccaccat gtgggtggtc
    11341 taccgggttc atcgcagtac cgcgaacggt cggacgaaca ccacgccagc gtgcagcacc
    11401 tgctttaccc agaacgcgca gcatatgctc agcattgcca acttcgccca gagttgcacg
    11461 gcagtctgct tctactttac gcatttcacc agaacgcaga cgcagggtga cataagcacc
    11521 atcacgagca acgatctgaa cgtaagtacc agcggaacgt gccagctgac cgcctttacc
    11581 tggtttcatt tctacgttat gaacagtaga accaaccggg atgttgcgca tcggcagggt
    11641 gttacctggt ttgattgcag catcaacgcc agactgaatc tggtcgccag ctttcaggcc
    11701 tttaggggcc aggatgtaac ggcgttcacc gtctttgtac agaaccagcg cgatgttcgc
    11761 ggaacggttc ggatcgtact caagacgttc aacaactgcc gggataccgt ctttgttgcg
    11821 tttgaagtca acaatacggt aagcctgctt gtggccacca ccgatatgac gagtggtgat
    11881 acggccattg ttgttacgac caccggattt gctgtttttt tccagcaacg gagcaaaagg
    11941 tttgcccttg tgcagctcag ggttaaccac tttaactacg tggcgacgac ccggagatgt
    12001 cggtttacat ttaacaactg ccattgtatt actcctccga cttactcagc gccgccaacg
    12061 aagtccagat tctggccttc tttcagggtg acgtaagctt ttttccagtc gctacgacga
    12121 ccgatacgct gtccgtgacg tttaactttc cctttaacta ccagggtgtt aacgacttcg
    12181 acttcgactt caaacagttt ctgcacagca gctttgattt ctgctttggt cgcgtcttta
    12241 gcaactttga gtacgatggt gttggatttt tccatcgcag tagacgcttt ttcagaaacg
    12301 tgcggtgcac gcagcacctt cagcagacgt tcttcacgaa tcatgccagc atctcctcaa
    12361 cttgcttaac agcatcagca gtcattacga ctttgtcgaa ggcgatcagg ctaaccgggt
    12421 cgataccagt tgcatcgcgt acgtcaacct tgtgcaggtt gcgcgcagcc aggaacaggt
    12481 tttcgtccag ctcaccggtg atgatcagca catcttccag agccatgtct ttcagtttct
    12541 gtgccagcag cttagttttc ggcgcttcta cagagaactt ctcgacaacg atcagacgat
    12601 cctgacgtac cagttcggac aggatgcttt tcagcgcgcc gcggtacatc ttcttgttaa
    12661 ctttttgact gtggtcctgc ggacgagcag caaaggtcac gccaccagaa cgccagatcg
    12721 ggctcttgat agaaccagaa cgcgcacggc cggtgccttt ctggcgccac ggttttttac
    12781 cggaaccagt tacttcagca cgagtcttct gagcacgagt accctgacga gcaccagctg
    12841 cataagcaac aacaacctgg tgaaccagcg cttcgttgaa atcacgaccg aaggtagttt
    12901 cggaaacagt cagcgcgctc tgcgcgtctt tcaatactaa ttccattgct atctccttac
    12961 gccttcacag ctggtttaac gatcaggtcg ctaccggttg cacccgggac agcaccttta
    13021 accagcagca ggttgcgctc agcgtcaacg cgtactacgt caaggctctg aacggttaca
    13081 cgttcgttac ccatctgacc tgccattttc ttgcctttga acactttgcc cggagtctgg
    13141 ttctgaccga tagaacccgg aacgcggtga gacaaggagt taccgtgagt agcgtcctgg
    13201 gtacggaagt tccagcgctt aacggtacct gcgaaacctt tacctttaga ggtgccagtt
    13261 acgtcaactt ttttaacgtc agcaaacagt tcaacgctaa tgctctgacc tacagtgaac
    13321 tcttcgcctt cagccaggcg gaattcccac agaccacggc cagcttctac gccagcttta
    13381 gcgaagtggc cagcttcagg cttggtcaca cggttagctt ttttagcacc ggtggtcacc
    13441 tgaatagcac ggtagccatc gttagccagg tctttaacct gagtaacgcg gtttgcttca
    13501 acttcgatta cggttactgg gatagaaacg ccgtcttctg tgaagatacg ggtcataccc
    13561 acttttttac cgactaaacc aatcattgtt tcaacctctc aatcgctcaa tgacctgatt
    13621 aacccaggct gatctgcacg tctacaccgg cagccagatc cagacgcatc agagcatcaa
    13681 cggttttctc ggttggctca acgatgtcaa ccagacgcaa gtgagtacgg atttcgtact
    13741 gatcgcgcgc gtctttgttg acgtgcgggg agatcagaac agtgaagcgc tctttgcgtg
    13801 tcggcagcgg gatcggacca cggacctgcg caccagtgcg cttggcagtc tcgacgattt
    13861 ccgcggttgc ttgatcgatc agacgatgat caaacgcttt caggcggata cggattcttt
    13921 ggttctgcat gagaccagag ctccaattat tttataaacg aaaatgatta ctcctcagac
    13981 ccattacgat tgatgggaga gtgtaaccgt tcttacgtag ctccccgatt gggagcattg
    14041 ttaggtagcc aaattcggct aactgaggtt cagattgaac ctgctgtcaa ctacgacaag
    14101 cccgcgcatt atacgcaaat ctgagcctga cgcaagcatc gggtagaaat taatcgcaac
    14161 gcgcagattg cgagctgcat tccacgaaat ttgtaaggtt gtgaagatag cgtttttcac
    14221 cggaatcagg ttaacaaatc agcgctctct ctgttgctga gcatccggag ttattcgatg
    14281 gtgatactcc aattacagag gatgttttat ggacactacc cttccctttc taattttgta
    14341 cgcttgttta tcagacctac tttttttctg ggatgcaaag catggcttgt tacctgacag
    14401 atttacgtgc ccgttactct ggtcaggtct tttgttctat caagtttgtc atcctgatgg
    14461 tttagccgat gcgttatggg gggcaattgt tggttacggc acattcgctg tcatttactg
    14521 ggggtatcgc atactgcgac ataaagaagg attaggctat ggtgatgtga agtttctcgc
    14581 cgcccttggc gcctggcatt catgggcctt cttaccacga ctggttttcc ttgctgcctc
    14641 attcgcttgc ggagccgtag ttattggtct gctcatgaga ggaaaagaat cattaaaaaa
    14701 cccgctgcct tttgggccat ttctggcggc tgcgggtttc gttataggct gggatagttt
    14761 gctgataggc cgttaatctt taactttaat ttgtgattgc aggtaatttt gtaggccgat
    14821 tttaccgatg agatccagtt cagtttcgag ccagtcgata tgaccttctt catcagcaag
    14881 gatctcgatc atcatatcgc ggctaacata gtcatgaacg ctgtcggcat atgcgatagc
    14941 ctcacgcaga tccttcgcgc cttccagttc taatcgcagg tcagattgca gcatctcctc
    15001 gacatcttca ccaatcccca gtttgcctaa atcctgtaag ttggggattc cttctaaaaa
    15061 caaaatacgc tcgatatatt tatcagcgtg tttcatctca tcgatggatt catggtattc
    15121 gacgtcattg agacgcatca ggccccagtt tttaaacatc cgggcatgga gaaaatactg
    15181 attgattgcg acaagctcat ttcccaatag tttattgaga taatttatga ttttaacatc
    15241 acctttcatt ttatagtccc tccgcttcca ctattagagc gtagatgggg ctagcgggat
    15301 gtcaaaaaat aagcgtgggc ttcaggcaat ctctttaaat tctggcatct gcatcaactc
    15361 gtcctgcatc acttcgcgcg cggcgcgaat gcacttaccg cattgatttc ccacaggaat
    15421 aaacttacgc aattgttgga aagattgcgg atgaaactga cggactgcct ggcgaatttt
    15481 tttgtcactt acaccattac acaaacaaac gtacatgatt gctcccgttc aaattctgca
    15541 caaagtgtaa atgagaatgg ttatgattac aatagcacag ttttatttgc gccatagttc
    15601 agcgagcaaa attacgacta aatatttcga gctcaaatta cgggcagcaa aaagggcgcc
    15661 gaagcgccct tttcaattca aaactaatta acgtgtaatt agcccagaac tttagcaaca
    15721 acgcccgcgc caacggtacg gccgccttca cggattgcga aacgcagacc gtcgtccatc
    15781 gcgatcgggt ggatcagggt aacaaccatt ttgatgttgt cgcccggcat taccatctct
    15841 acgccttccg gcagttcgat ggtaccagtc acgtcagtag tacggaagta gaactgcgga
    15901 cggtagcctt tgaagaacgg agtatgacgg ccgccttcat ctttggacag aatgtacact
    15961 tcagattcga acttggtgtg cggcttgatg gtgcccggct tagccagtac ctgaccacgt
    16021 tcgatttctt cacgtttgat accacgcagc agaataccta cgttctcacc agcacggcct
    16081 tcgtccagca gtttgcggaa catttcaacg ccagtacagg tagacttctg agtctctttg
    16141 ataccaacga tttcaacttc ttcaccaact ttgatgatac cgcgttctac acgaccggta
    16201 acaacggtac cacgaccgga gatggagaat acgtcttcga tcggcagcag gaacggcttg
    16261 tcaatcgcac gctctggttc cggaatataa gaatccagga agccagccag ttccaggatt
    16321 ttcgcttccc actctgcgtc gccttccagc gctttcagag cagaaccacg aacgatcgga
    16381 gtgtcgtcgc ccgggaagtc gtactgagac agaagttcac gaacttccat ttcaaccagt
    16441 tccagcagct cttcgtcatc aaccatgtcg catttgttca ggaacacgat gatgtacgga
    16501 acgcctacct gacgacccag caggatgtgc tcacgagtct gcggcatcgg gccgtcagtc
    16561 gcagcaacta ccaggatcgc gccgtccatc tgagcagcac cggtgatcat gtttttaaca
    16621 tagtcggcgt gccccgggca gtctacgtgt gcgtagtgac gggtcggggt gtcatattca
    16681 acgtgagaag tgttgatggt gataccacga gctttttctt ccggcgcgtt atcgatctgg
    16741 tcgaatgcac gagcagcacc gccgtaggtt ttagccagta cggtggtgat tgcagcggtc
    16801 agagtagttt taccgtggtc aacgtggccg atagtaccaa cgttaacgtg cggttttgta
    16861 cgttcaaatt tttctttaga cacggctata ttccttacta tagtgctctc cccttcagga
    16921 gagagcacgg gactttggta ttaaccctta ggcttattta ccacgggctt caattacggc
    16981 ctgagcaacg ttactcggcg cttcatcata cttcaggaat tccatagtgt atgatgcacg
    17041 acctttggtc agagaacgca gctgagttgc gtatccgaac atttcagaca gcggtacttc
    17101 agcgtggatc ttaacgccag taacttcaga ttcctgacct ttgagcatac cacgacgacg
    17161 gctcaagtca ccgataacgt caccggtgtt ctcttccgga gtttctactt caaccttcat
    17221 gatcggctca agcagaactg gtttcgcttt cttaaagcct tctttaaagg cgatagaagc
    17281 agccagttta aacgccagtt cagaggagtc aacgtcatgg taagaaccga agtgcagacg
    17341 aatacccatg tctactaccg ggtagcctgc cagcggacct gctttcagct gttcctggat
    17401 acctttatca acggccggga tgtattcgcc agggattaca ccacctttaa tgtcgttgat
    17461 gaactcgtag cctttcgggt ttgaacccgg ctccagcggg tacatgtcga taacaacatg
    17521 accatactga ccacgaccac cagactgttt cgcgtgttta ccttcaacat cggtaacttt
    17581 ctggcggata gtttcacggt aagcaacctg cggtttacct acgttcgctt caacgttgaa
    17641 ttcacgcttc atacggtcaa cgatgatgtc gaggtgcagt tcgcccatac ccgcgatgat
    17701 ggtctggtta gattcttcgt cagtccatac acggaaagac gggtcttctt tagccagacg
    17761 gcccagagcc agacccattt tttcctggtc agctttggtt ttcggttcaa ctgcgatgga
    17821 gattaccggc tcagggaatt ccatacgttc cagaatgatc ggcgcatccg ggtcacacag
    17881 ggtgtcacca gtggttacgt ctttcagacc gatagcagca gcgatgtcgc ccgcgcgaac
    17941 ttctttgatc tcttcacgtt tgttagcgtg catctgaacg atacgaccga aacgctcacg
    18001 tgcagctttc acggagttca gtacggtatc accagagtta accacaccgg agtaaacacg
    18061 gaagaaggtc aggttaccaa caaacgggtc ggtagcgatt ttgaacgcca gtgcagagaa
    18121 cggctcgtca tcacttgcgt gacgttcagc cggagtgtct ttaccgtcgt ccaggatacc
    18181 gttgatcgca ggtacgtcaa ccggggatgg caggtaatca attaccgcat ccagcatcgc
    18241 ctgaacacct ttgttcttga acgcagaacc acaggttacc aggatgattt cgttgttcag
    18301 aacgcgctga cgcagagcac ctttgatttc tgcttcagtc agttcttcac cacccaggta
    18361 tttttccatc agctcttcag aagcttcagc tgcggattcg atcaggttct ggtgccattc
    18421 gttagccagt tcaaccatgt ctgccgggat atcttcgtat tcgaaggtta cgccctggtc
    18481 agcgtcgttc cagttgatag ctttcatttt caccaggtca acaacaccgg tgaaatgttc
    18541 ttcagcacca atcgccagct gcagcggaac cgggttcgcg cccagacggg ttttgatctg
    18601 gttaacaact ttcaggaagt tcgcacccat gcggtccatt ttgttaacga acgcaatgcg
    18661 cggaacttta tatttgtttg cctgacgcca tacggtttca gactgcggct gaacaccacc
    18721 aactgcgcag taaaccatta ccgcaccatc gagaacacgc atggaacgtt ctacttcgat
    18781 tgtgaagtca acgtgccccg gggtgtcgat gatgttgatg cgatgcggct catactgctt
    18841 agccatacca gaccagaatg cagtagtcgc agcggaagtg atggtaatac cacgttcctg
    18901 ctcctgctcc atccagtcca tggttgcagc gccgtcatga acttcaccga ttttatggtt
    18961 tacaccggtg tagaacagaa tacgttcggt agtagtggtt ttaccggcgt cgatgtgcgc
    19021 actgataccg atgttacggt agcgtgcgat gggtgttgta cgagccattt gtttcctcgt
    19081 ttatctttta ggcgttcaat ttaagtagcc caaagcgggc tgcttactgg aagcgcccgc
    19141 ctggtgacta aaactccgaa gggattacca acggtagtgt gcgaacgcct tgttggcttc
    19201 ggccatacgg tgaacgtctt cacgtttctt aactgcagta cctttgtttt ctgcagcatc
    19261 agaaagttcg ttcgccaggc gcagagccat ggatttatca ccgcgtttac gagcagcttc
    19321 aacgatccaa cgcattgcca gagcattacg acgaaccgga cggacttcaa ctggtacctg
    19381 ataagtagaa ccaccaacgc ggcgagactt aacttctaca gtcgggcgca cgttttcgag
    19441 agctacttcg aatgcttcca gttcagattt accagagcgc tgagccaggg tctccagcgc
    19501 gctgtatacg atagattcag cagtagattt tttaccatct accatcagga tatttacaaa
    19561 tttagccagc agttctgatc cgaacttcgg atccggcaga attttacgct gaccaatgac
    19621 gcgacgacgt ggcatggaaa tactccgttg ttaattcagg attgtccaaa actctacgag
    19681 tttagtttga catttaagtt aaaacgtttg gccttactta acggagaacc attaagcctt
    19741 agggcgcttc acgccatact tggaacgagc ctgcttacgg tctttaacgc cggagcagtc
    19801 aagcgcacca cgtacggtgt ggtaacgaac acccgggagg tctttaacac gaccgccacg
    19861 gatcaggatc acggagtgct cctgcaggtt gtgaccttca ccaccgatgt aggaagtcac
    19921 ttcgaaaccg ttagtcagac gaacacggca tactttacgc agcgcggagt tcggtttttt
    19981 aggagtggta gtatatacac gagtacatac gccacgtttt tgcgggcatg cttccagcgc
    20041 aggcacgttg cttttcgcaa ctttgcgagc acgtggtttg cgtaccagct ggttaactgt
    20101 tgccattaaa tagctcctgg ttttagcttt tgcttcgtaa acacgtaata aaacgtcctc
    20161 acacaatatg aggacgccga attttagggc gatgccgaaa aggtgtcaag aaatatacaa
    20221 cgatcccgcc atcaccaggc catctggctg gagtgcttaa ccgtaagtct gacgaaatca
    20281 gtatagtcaa tgggaatgat gtcgttcaaa atttgaccag tcaaaccgcg ggcaataagg
    20341 tcttcgttca gggcatagac cttaatgggg gcattacgca gactttcaag gtagcggtta
    20401 ccgtcaactg cggcagttac gccatcttgc aataatagca gttcgtctcc ttcactgagc
    20461 agacgcagca gcgcagcaaa atccgtcagc cagggtgaac gatgtaacgt gtgcagcata
    20521 aacgcctcaa aacctcaaaa taacatcgta gttggcgagt tcgcggcgta aggcatctgc
    20581 ttcgagcggc gtggcttcga caacaaaggg tgtctgcgga tctaacccgc gttcgcgcag
    20641 tgaagccgca caaacccagc actgttcaat gtcgtacaga ctcaacaatt taaaagtggc
    20701 aatgtaatca cgcgccagca ctgcatcggg cttttgtcct gacagcagct gaaaaacgcc
    20761 atcagcaata aagaagacgg ccagatcgtc agttaatgcg gaagttgcca gtaaagcatc
    20821 taaaccttcc cgaccagcag ctgtaccatg aggtgcagta gaaaaaacaa acgcaattcg
    20881 tttcatcaga actgtaccac cctgtcacag gtcagcgagg cttccgccag cgcgccaagt
    20941 ccgcttaagg taaatcccgg ctgaaggttt gacgaagcca gccccagcct tcctgcttca
    21001 gtttcatcga cgataccgcg gcgtaacgct gccgctacgc agatattcag cgccacacca
    21061 tgttgcgcat tcaactgttg ccagccgcgc acaaggtcaa attcgtcgct tgccggtgag
    21121 gtcagttggt tagcgttata gaccccttcc cgatagaaaa agacgctgct taacttatgg
    21181 ccttctgcta tcagcgcctg cgcaaactga aaagcactac tcgcctgttg cgtaccgtat
    21241 gctggcccgg tcaccacgat ggcaaaacgc attacttatc ttgcccctga aaatcaccgc
    21301 tcttgaactg gcggatgtag agatagacag tgtgtttgga gatgttcagg cggtcagcaa
    21361 cctggttgat cgcatcttta atatcgaaga tccctttctc gtagagattc agcacgatct
    21421 gacgattttt ggcgttatta gaaacattgc gatcggcatt cacttcttcg atggtgaact
    21481 ccagcgtttg ggtaaccaga tcttcaacag aagaggcaaa gttgacgctt gaaccgacat
    21541 ccggggtttc tggcggcaca aaagtgctca taatctgcga gaagggaaca tcaagattca
    21601 tattgatgca cagcagacca attacacgct gttcgcggtt acgaatcgcg atagtcaggg
    21661 acttcattaa tacgccgctt ttggcgcgag taaagtagca tttagaaacg ctgctatccg
    21721 ctcccgtcat atcgtgcagc atacgtagcg ccaggtcagt aattggcgaa ccaatcttcc
    21781 ggcctgtatg ttcaccgtta gcaatgcgaa tggctgaaca ttttagatcc tgcaaagagt
    21841 gcaaaacgat ttcacagtgg gagccaataa gcatcgctaa cccgtccacc accgcttcgt
    21901 aggatttcag aatatcaaaa tcggtctggt cgaaaggacg ttgatccagt aaatccaact
    21961 cactggtttc gttggttaaa agcgacctgg acatgaaaaa aaacactcct tttcaggagc
    22021 ctgtcgttaa cttttcaggg caggctcatt aatgatgcgg gtaactaaat taatacagcg
    22081 gaggttccgc tttccagcac taattatatc cggcctgtaa taaaaaaacc gccgcctggt
    22141 caggcggcgg ttcttaatgc ttatttttta gcagaatctg cggctttcgc atcagcttcc
    22201 ggctttgcat cagccttcgg cgctggtttc acatccagca gctctacgtc aaacactagg
    22261 gtagaattcg gtgggatccc cggaacaccc gctttgccgt aagccagttc tggtggaata
    22321 accagtttga tcttaccgcc tttcttgatg ttcttcagac cttctgtcca acccgggata
    22381 acaccgtcca gacggaaaga aagcggttca ccacgggtgt aagagttgtc gaactcttta
    22441 ccgtcgatca gcgtaccttt gtagttcact acaacagtat cgctgtcttt cggtgcttcg
    22501 cctttaccgg cttctactac ctgataaacc agaccagttg aagaggtttt cacacctttc
    22561 tctttggcaa atttctcgcg gtactcttta ccttttgctt cgttatcagc cgcgtctttt
    22621 tccatcttcg cctgagcaga agacttcacg cgagcttcga atgcttgcag agtctgttcg
    22681 atctcttggt cggagagttt gctcttatca gcaaatgcat cctgaacacc agcgatcagc
    22741 tgatctttat ccagtttgat gcccagtttt tcttgttctt taagagagtt ttccatgtaa
    22801 cgacccagcg aagcacccag tgcataagct gatttctgat cgtcattttt gaacgctgct
    22861 ttgctgtcag cagttgtagc aggttttgca gcttcagcag caaaagtgat tggtgcatgc
    22921 agggcaacgg ccattgtggt cgccagtagc gttactttaa acagtgattt catccatatc
    22981 tccaggatcg gggcatctca ccccagggtt aactattatc agaagggtac tataaagcgt
    23041 tgtcgaacaa atctacatac agacacgccc tattatcatc tattttcaga ctctttttgt
    23101 ttaaattagt ttcgatgacc gcgaaatgag tgctgtctcg ggcagcaaag ttaagtagaa
    23161 tccgcggcga ccattcgaca aaagaggtga atcatgcagg atttatcatt ggaagcacgc
    23221 ctggcagagc tggagagccg actggctttt caggaaatca ccattgaaga actgaacgtc
    23281 acggtgaccg ctcatgaaat ggagatggcg aaactgcgcg atcatctgcg tctgctgacc
    23341 gagaagttaa aagccagcca gccgtcgaac atcgcgtcgc aggctgaaga aacgccaccg
    23401 ccacattatt gaggcgtaaa aaaagcgggg attccccgct tttttgtcac cttttcggta
    23461 ttagtggcaa ccacaaccgc cgttgccttt accgccacag cagccttcgc caccgtgttc
    23521 atgaccgtga tcgtggccat gaccgccgca gcaaccgtcg tggtcgtgat cgtggtgatg
    23581 atcgtgcgcg ccgtgaacgt ggccatgagc cagttcttct tcggtcgctt cgcgaatcgc
    23641 cacaacttca acgttgaatt tcaggttctg accggccagc atgtggttac catcaaccac
    23701 tacgtgatcg tcttcaaccg cagtgatttc aaccggtacc ggaccctggt cagtttcagc
    23761 caggaaacgc atacctacct gcagttcatc aacgcccata aatacgtctt taggaacacg
    23821 ttgcactagg ttttcgtcgt actgaccgta agcgtcgttc gcgccaacag cgacatcaaa
    23881 tttgtcgcca acttcatgac cttccagcgc cgtttccagg ccagagatca gggaaccgtg
    23941 accatgcagg tagtccagcg gcgcactcac cggagactca tcaaccaaca caccgtcttc
    24001 tgtacgtacc tgataggcca ggctgaccac caggtctttt gctactttca tgatatctcc
    24061 tgagcatggg aagaatagtg gcgcagattg tagcggaatt ctgcagccgt gtactcacta
    24121 gcttaaaaaa actcggcgca tatcgctagt ccggatgaaa aatcccgatc acttgctcat
    24181 ctttgtgaac gtgatcgcgg gcttctttgt ctgcttctcg catctgatgt ccgcacttaa
    24241 cacattcaac aatatcaata ttattttcgc gccacatcgc cattgaatcc tgcgcctgac
    24301 aggccgggca ttttgcgccc gctataaaac gttttcggat tgccatcctt accctctact
    24361 caaattcatc ccagccgtcc agctggcgtc gttcttgttg catttcacgc tggaaaatct
    24421 cttccagttc gcgtcgggct tccctggcgc gagaaatttg tacggtatca gcatgcatcg
    24481 ggattaactc ccgcagcatt cgcatatcca ggcggcgaaa atgcagttgc gcccgctgtg
    24541 cctgatgcgg atgcatgcca agcgtgacca gcgtcttgcg ccccagctct aacgcactgg
    24601 agaatgtttc acgggaaaac tgcgtcaccc ctgcctgtaa taactcatgc gcttccacac
    24661 gtccgcgcac tcgcgcaaga atatgcaaat gcggaaagtg ctgctggcat atttccacca
    24721 gcttcatggt gtcttccggc tcgttacagg taatgacgat agactcagcg gcctctgcac
    24781 ccgcagaacg taaaagatcg acctgcgtgg cgtcgccgta ataaactttg tagccatatt
    24841 tacgcatcag gttaacggcg ctgatatccc gctccagcac ggtaatgcgc attttatttg
    24901 ccatcagcaa acgaccaatc acctgaccaa aacgcccgaa gcccacgaca atgacctggg
    24961 gtttatcatc gttgacccac ggtttttcgt cttcttcttc cggtccgtta aactggcggg
    25021 atagccattt atccaccagc ttcatcagca acggcgtggt catcatggaa agcgtcaccg
    25081 tcaccagcaa cagcgccatc tggtcgccct ggaataaacg ttgtgaagaa gcggtagaaa
    25141 agaggacaaa ggcaaactca ccgccctgac tcaacacgcc agcaaactgc atccgctctg
    25201 agctacgcac gccatacaat cgcgccagca gatacagcac gagaattttc accgccacca
    25261 gcacaaccac gcttatcact acccacaaca gatgggtata aagcaccccg aggttgagcg
    25321 acatgccgac agagataaag aacaaaccga gcagcaagcc tttgaaggga tcgatagccg
    25381 tttccagttc atggcgatat tcactttccg ccagcagcac gcccgcaata aatgtaccga
    25441 gcgccatcga cagccccagc gcatccataa acaatgcgga acccaacacc agcagcagcg
    25501 tcgcggcggt gaacacttcc cgcacgccag aagctgcaat aaagcggaat accggacgca
    25561 gtaaatagcg cccaccaatc agcatgccga caaacgccag caccttcatg ccgatcttca
    25621 tccagtcgaa atgttcgtct gccgaccccg ccaacaacgg cactaacgcc agtgctggga
    25681 tcactgccag atcctgaaac agcagaaccg aaaacccgag ctggccggat tcgctgcgat
    25741 tcatcccttt ctcacgcatc aattgcaacg ccattgcagt tgaagacatc gcaaggccaa
    25801 tgccaccgac caccgccgcc tgccaggcga aatccgtcag catcaataat cccgccagca
    25861 acgccgcgct taacagcacc tgtgccacgc ctacgccaaa aatcgaacgc cgcagttgcc
    25921 aaagtttgga gggattcaac tcaaggccga tgataaacat caggaatacc acgccgagtt
    25981 ccgaaaagtg gaggatctca tcgacgtcgc taataaaccc cagcccccac gggccaattg
    26041 caatccctgc cagcaaatat cccaacacag cgccaatccc cagccgcgat gccaacggca
    26101 ccgcagccac cgccgcgaag agaaacagca ctcctgcgag taaaaaatcg gaaccttcca
    26161 tcagcggcct cctggagaca gcggatttgc caaccagtca ccgtaggctc tggcgtggct
    26221 cgccagctcc tttgcgcttt gccgtctcgc ccagtaaata atgatggaac ttaaccaatg
    26281 catccggcac atgcccgccg ccagttcaaa ggggcgcagc acatcgctca tcgggtagcg
    26341 attcagcgcg tcataacggt aagcactttc cggctcgccg gtggtaatca cgttacgcca
    26401 gtactttccc gccagttggt ttcctcccgg cccgctggca aaaccacgac ttaatacccg
    26461 gtccagccac tctttcagta gcgccgggca gctataggta taaagaggat gctgaaagac
    26521 aatcacctcg tgctcgcgca gtaatgcctg ctcacggggg atatcaataa aaaaatcggg
    26581 atagtgcgcg taaaggtcgt gcacggtaac attgctgagc tgcgtggccg gtttaagcag
    26641 tacccggttt gccaccgagt cctgagattc cggatgggca tacagcagca aaactttcgc
    26701 tggctgagac atcatccccc tcccggtatg gtttttgtgt atagtcgctg ttttgggcta
    26761 ccattgcgcc cggtgcggca gctcgcccat acattacatt atcataatga taagttaaca
    26821 tagtctgaac atacggcgcc ttatgattgt tttctcctcg ttacaaattc gtcgcggcgt
    26881 gcgcgtcctg ctggataatg ccaccgccac catcaacccc gggcagaaag tcggcctggt
    26941 gggtaaaaac ggctgtggta aatctaccct gctggcattg ctgaaaaatg aaatcagcgc
    27001 cgacggcggc agctacacct ttccgggaag ctggcaactg gcgtgggtga atcaggaaac
    27061 gccagcgtta ccgcaagcgg cgctggaata tgtcattgac ggcgaccgtg aatatcgtca
    27121 actggaagcg cagctgcacg acgccaacga acgtaacgac gggcacgcca ttgcgaccat
    27181 tcatagcaag ctggatgcta ttgacgcatg gagtattcgc tcccgtgccg ccagcctgct
    27241 gcacggcctc ggtttcagca atgaacaact ggagcgcccg gtaagtgatt tctccggtgg
    27301 ctggcgtatg cgtcttaacc ttgcccaggc gctgatttgc cgttcagact tgctgctgct
    27361 cgacgaaccg actaaccacc tcgatctcga tgccgttatc tggctggaaa aatggttgaa
    27421 gagctatcag ggcacgctga tcctgatctc tcacgaccgc gacttcctcg atccgatcgt
    27481 tgataaaatt attcagatcg aacaacaaag catgttcgag tacaccggca actacagttc
    27541 gtttgaagta cagcgcgcca cccgtctggc gcagcaacaa gcgatgtacg aaagccagca
    27601 ggaacgcgtg gcacatctgc aaagttatat cgatcgtttc cgtgccaaag ccaccaaagc
    27661 gaagcaggcc cagagccgca ttaagatgct ggaacgtatg gagctgattg cccccgcaca
    27721 cgtcgacaac ccgttccgct ttagcttccg cgcgccggaa agcctgccaa atccgttact
    27781 gaagatggaa aaggtcagcg caggctatgg cgatcgcatt attctcgact cgattaaatt
    27841 gaatctggtc cccggctcgc gcattggtct gttaggccgc aacggcgcag gtaaatcgac
    27901 attaatcaaa ctgttagccg gtgaacttgc gccagtcagc ggtgaaattg gtctggcgaa
    27961 agggatcaag ctcggctact tcgcccagca tcaacttgaa tacctgcgcg ccgacgaatc
    28021 acctattcaa catctggcac gtttagcgcc gcaggagctg gaacaaaaac tgcgtgacta
    28081 cctcggcggc tttggtttcc tgggcgataa agtaaccgaa gaaacgcgcc gcttctccgg
    28141 tggggaaaag gcccgcctgg tgctggcatt aattgtctgg cagcgtccga atctgctgct
    28201 gctcgacgaa ccgactaacc accttgacct cgacatgcgt caggcagtca ccgaagcatt
    28261 aatcgagttt gaaggcgcgc tggttgtcgt ttcgcacgac cgtcatttgc tgcgttccac
    28321 cactgacgat ctctacctgg ttcacgatcg taaagtcgaa ccgttcgacg gcgatctgga
    28381 agattatcaa cagtggttga gcgacgtaca aaagcaggaa aaccagaccg acgaagcgcc
    28441 aaaagagaac gcgaacagcg cccaggcacg taaagatcag aagcgtcagg aagcggagct
    28501 gcgtgcgcaa acccagccgc tgcgtaaaga gattgcccgt ctggaaaaag agatggagaa
    28561 gctgaacgcg caactggcgc aggcggaaga gaaactcggc gacagcgaac tgtatgacca
    28621 gagccgtaaa gcggagttga ccgcctgcct gcaacagcaa gccagcgcca aatccggcct
    28681 ggaagagtgc gaaatggcgt ggctggaagc ccaggagcag cttgagcaga tgctgctgga
    28741 aggccaaagc aactgatggc gcagataacg acgaccgatg ccaatgaatt tagcagcagt
    28801 gctgaattca cccctatgcg cggctttagc aattgtcatc tacaaactat gctgccgcgt
    28861 ttgtttcgtc gtcaggtgaa attcaccccg tactggcagc ggctggagtt gcccgacggc
    28921 gattttgtcg atctcgcgtg gagtgaagac cccgcacagg cgaaacataa accgcgttta
    28981 gtggtgtttc acgggctgga aggcagtctc aatagccctt acgcccacgg tctggttgag
    29041 gcggcgcaaa agcgcggctg gctgggcgtg gtgatgcatt ttcgcggatg cagcggtgaa
    29101 ccaaaccgta tgcaccgcat ttaccattcg ggcgaaaccg aagacgccag ttggttttta
    29161 cgctggctgc aacgcgaatt tggtcatgcg ccaacggctg ccgtcggcta ttcgctcggc
    29221 ggtaatatgc tggcctgttt gctggcaaag gaaggcaatg atctcccggt tgatgcggcg
    29281 gtgattgtct ctgcgccgtt tatgctggaa gcctgtagct atcatatgga aaagggcttt
    29341 tcccgcgttt atcagcgtta cttgctgaac ctgttaaaag ccaatgccgc gcgcaagctg
    29401 gcagcctacc ccggaacgct gccgattaat ctcgcgcagt taaaatcggt acgtcgcatc
    29461 cgtgaatttg acgatctaat caccgccaga attcacggct acgccgacgc tatcgactat
    29521 tatcgtcagt gtagcgccat gccgatgctg aaccggatcg ccaaaccgac gctgattatt
    29581 cacgccaaag acgatccgtt tatggatcat caggtgatcc caaaaccgga aagtctcccc
    29641 tcgcaggtgg agtatctact gactgaacat ggcggtcatg ttggctttat tggcggtaca
    29701 ttacttcatc cgcaaatgtg gctggagtca cgcattcctg actggttaac aacgtatctg
    29761 gaggcgaaat catgttgatt ccgtggcaag acctctcccc cgaaacgctg gaaaatttaa
    29821 ttgaaagctt tgtgttacgt gaaggcaccg attatggtga acatgagcgt acacttgaac
    29881 agaaagtcgc cgacgtcaaa cgccagctac agtgcggaga agcggtgctg gtatggtcgg
    29941 aactgcacga aacggtcaat attatgccgc gcagtcagtt tcgcgaataa cctgcaaccg
    30001 tcggcggacg agataatagt gatagctaag tcatggagtt actatgtctg ccaaacatcc
    30061 ggtcattgcg gtaacaggat ccagcggcgc ggggaccacc accaccagcc tcgcgtttcg
    30121 taaaatattc gcgcagttaa atctgcatgc agctgaggtg gaaggcgaca gttttcaccg
    30181 ttacacccgc ccggaaatgg acatggcgat ccgcaaagcg cgcgacgccg ggcggcatat
    30241 cagctacttc ggccccgaag cgaacgactt cggcctgctg gaacaaacct tcattgaata
    30301 cggtcagagc ggcaaaggga aatctcgcaa atatctgcat acctacgacg aagccgtacc
    30361 ctggaatcag gtaccgggaa cattcacccc ctggcaacct ttaccagaac ccactgatgt
    30421 actgttttat gaaggtttac acggcggcgt agtcacgcca cagcataacg ttgcgcagca
    30481 tgtggactta ctggtcggcg tagtgcctat cgttaacctt gagtggattc aaaaactgat
    30541 ccgcgacacc agcgagcgcg ggcactcacg agaagcagtg atggactcgg tagtgcgttc
    30601 aatggaagac tatatcaact acatcacacc gcagttttcc cgcacccatc ttaacttcca
    30661 gcgcgttccc accgtcgaca cttcaaaccc gttcgcggca aaaggtatcc cgtcgctgga
    30721 tgaaagcttt gtggtgatcc attttcgtaa tctggaaggg atcgatttcc cttggctgct
    30781 ggcgatgttg caaggctcat tcatttccca catcaatacg ttagtggtac ctggcggcaa
    30841 aatggggctg gcaatggaat taattatgct gctgctggtg caacgattga tggaaggaaa
    30901 gaaaatcgag taactttgct attatgccgg ataaaatact atccggcttc acaacgggat
    30961 agttaagtca cgcggcaacc acttcatacg agtgagtaat attcaccgct ttttccagca
    31021 tcaacgccac tgaacaatat ttctcggcag agagatcaac cgcacgcgca accgctgcgt
    31081 ctttcaggtc gcgaccggtg acgataaaat gcagattaat gtgcgtaaac aggcgtggtg
    31141 cctcttcgcg gcgttcagag gtcaatttta cttcacaatc gaccacatcc tgacgccctt
    31201 tttgcaggat cgaaaccaca tcgatggcac tgcaaccacc cgccgccatc aacaccattt
    31261 ccatcggact tggtgcttta tcgcctgagt tgccgtccat taaaatctgg tgaccagagg
    31321 cggattcgcc cagaaaagtt aacccttcga cccacttcac tcgcgcttgc atttttgcta
    31381 ctccactgcg tcaattttcc tgacagagta cgcgtactaa ccaaatcgcg caacggaagg
    31441 cgacctaggt catgctgaag cgagacacca ggagacacaa agcgaaagct atgctaaaac
    31501 agtcaggatg ctacagtaat acattgatgt actgcatgta tgcaaaggac gtcacattac
    31561 cgtgcagtac agttgatagc cccttcccag gtagcgggaa gcatatttcg gcaatccaga
    31621 gacagcggcg ttatctggct ctggagaaag cttataacag aggataaccg cgcatggtgc
    31681 ttggcaaacc gcaaacagac ccgactctcg aatggttctt gtctcattgc cacattcata
    31741 agtacccatc caagagcacg cttattcacc agggtgaaaa agcggaaacg ctgtactaca
    31801 tcgttaaagg ctctgtggca gtgctgatca aagacgaaga gggtaaagaa atgatcctct
    31861 cctatctgaa tcagggtgat tttattggcg aactgggcct gtttgaagag ggccaggaac
    31921 gtagcgcatg ggtacgtgcg aaaaccgcct gtgaagtggc tgaaatttcg tacaaaaaat
    31981 ttcgccaatt gattcaggta aacccggaca ttctgatgcg tctgtctgca cagatggcgc
    32041 gtcgtctgca agtcacttca gagaaagtgg gcaacctggc gttcctcgac gtgacgggcc
    32101 gcattgcaca gactctgctg aacctggcaa aacaaccaga tgctatgact cacccggacg
    32161 gtatgcaaat caaaattacc cgtcaggaaa tcggtcagat tgtcggctgt tctcgtgaaa
    32221 ccgtgggacg cattctgaag atgctggaag atcagaacct gatctccgca cacggtaaaa
    32281 ccatcgtcgt ttacggcact cgttaatccc gtcggagtgg cgcgttacct ggtagcgcgc
    32341 cattttgttt cccccgatgt ggcgcagact gatttatcac cccgatatca actatgcact
    32401 tcgacaaacg ctggtgctat gtttgcccgt ggccgttggg ttaatgcttg gcgaattacg
    32461 attcggtctg ctcttctccc tcgttcctgc ctgttgcaat attgcgggcc ttgatacgcc
    32521 tcataaacgt tttttcaaac acttaatcat tggtgcgtcg ctgtttgcca cctgtagctt
    32581 actgacacag ctgctactgg taaaagatgt tcccctgccc tttttgctga ccggattaac
    32641 gctggtactt ggcgtcactg ctgagctggg gccattgcac gcaaaattgc ttcctgcatc
    32701 gctgctcgcc gccattttta ccctcagtct ggcgggatac atgccggtct gggaaccatt
    32761 gctcatctat gcgttgggca ctctctggta cggattgttt aactggtttt ggttctggat
    32821 ctggcgcgaa caaccgctgc gcgagtcact aagtctgctg taccgtgaac tggcagatta
    32881 ttgtgaagcc aaatacagcc tgcttaccca gcacaccgat cctgaaaaag cgctgccacc
    32941 gctgctggtg cgccagcaaa aagcggtcga tttaattact cagtgctatc agcaaatgca
    33001 tatgctttcc gcgcaaaaca ataccgatta caagcggatg ctgcgtattt tccaggaggc
    33061 gctggatttg caggaacata tttcggtcag tttgcatcag ccggaagagg tgcaaaaact
    33121 ggtcgagcgt agccatgcgg aagaagttat ccgctggaat gcgcaaaccg tcgccgctcg
    33181 cctgcgcgtg ctggctgatg acattcttta ccatcgcctg ccaacgcgtt ttacgatgga
    33241 aaagcaaatt ggcgcactgg aaaaaatcgc ccgccagcat ccggataatc cggttgggca
    33301 attctgctac tggcatttca gccgcatcgc ccgcgtgctg cgcacccaaa aaccgctcta
    33361 tgcccgtgac ttactggccg ataaacagcg gcgaatgccg ttgcttccgg cactgaaaag
    33421 ttatctgtca ctaaagtctc cggcgctacg caatgccgga cgactcagtg tgatgttaag
    33481 cgttgccagc ctgatgggca ccgtgctgca tctgccgaag tcgtactgga tcctgatgac
    33541 ggtattgctg gtgacacaaa atggctatgg cgcaacccgt ctgaggattg tgaatcgctc
    33601 cgtgggaacc gtggtcgggt taatcattgc gggcgtagcg ctgcacttta aaattcccga
    33661 aggttacacc ctgacgttga tgctgattac caccctcgcc agctacctga tattgcgcaa
    33721 aaactacggc tgggcaacgg tcggttttac tattaccgca gtgtataccc tgcaactgtt
    33781 gtggctgaac ggtgagcagt acatccttcc acgtcttatc gataccatta ttggttgttt
    33841 gattgctttc ggtggtaccg tctggctgtg gccgcagtgg cagagcgggt tattgcgtaa
    33901 aaacgcccat gatgctttag aagcctatca ggaagcgatt cgcttgattc tgagcgagga
    33961 cccgcaacca acgccactgg cctggcagag aatgcgggta aatcaggcgc ataacactct
    34021 gtataactca ttgaatcagg cgatgcagga accggcattc aacagccatt atctggcaga
    34081 tatgaaactg tgggtaacgc acagccagtt tattgttgag catattaatg ccatgaccac
    34141 gctggcgcgg gaacaccggg cattgccacc tgaactggca caagagtatt tacagtcttg
    34201 tgaaatcgcc attcagcgtt gtcagcagcg actggagtat gacgaaccgg gtagttctgg
    34261 cgatgccaat atcatggatg cgccggagat gcagccgcac gaaggcgcgg caggtacgct
    34321 ggagcagcat ttacagcggg ttattggcca tctgaacacc atgcacacca tttcgtcgat
    34381 ggcatggcgt cagcgaccgc atcacgggat ttggctgagt cgcaagttgc gggattcgaa
    34441 ggcgtaatac aggctaaacc atcggataag gcattcacgc cgcatccgac atattttgcc
    34501 tgatgcgtct tatttacgcc ccaaccacct tcgccaccgc gtgggcgaaa cgttgcatcc
    34561 cctcatcgat atccgcatct tccaccacca gcgacggtgc taaacgcatc acatccggtc
    34621 cggcattcag caccattacg ccaacctctg cacccgcata caggaaatca cgcgcccgac
    34681 ctttgtagtg tggtttcagc tccgcgccaa ttaacagccc cataccgcga atatcgctaa
    34741 acacatcgta ctgctgatcg atcttctgca aatggtcaac aaaacgctgg cgtttcgcct
    34801 gaatgccttc cagcacttca ggggtattga tgatatcaaa cgccgcgccc gctactgcac
    34861 aggccagagg attaccgccg taggtggaac cgtgagaacc aggatgaaac gcagaagcaa
    34921 tttccgccgt ggtcagcatg gcgctaatcg ggaagccgcc gcctaacgct ttcgcagaag
    34981 tcagaatatc cggcgtcaca ccgtagtacc tgtaagcaaa caaatcgcca gtccgcccca
    35041 tcccgcactg cacttcatca aacaccaata atacctgatg ttgatcgcac agctcgcgca
    35101 agccctgcaa aaactctggc gtcgctgccg tcacaccgcc ctcgccctgg atcggctcaa
    35161 ccaccaccgc acaggtgtga tcatccatca ccgctttcac tgcatggaga tcgttaaagg
    35221 gaacgtggat gatgtctgcc ggttgcggcc caaagccgtc ggaatatttt ggctgcccac
    35281 ccaccgaaac ggtaaacagc gagcgaccat gaaaagcgtt atggaaggca ataattttgg
    35341 ttttgaacgg gctatgacgc acacaggcgt aatggcgtgc cagtttaaag gcggtttcgt
    35401 tagcttccgt gccggagttc ataaacacca cgcgttcggc aaacgtcgcc tcaatcagtt
    35461 tgcgcccaag acgcagcgcc ggttcattgg tgaaaacgtt actgatatgc cacagagttt
    35521 cgccctgggt ttttaacgcg ttcaccagcg caggatggca atggcccaac gccgtaactg
    35581 caatgccacc cgcgaaatcg acatactcct tgccttgctg atcccagatt cggctgccct
    35641 gaccttttac cggaataaac tctgccggag cataaatcgg caggatcact tcatcgaaag
    35701 tcgcgcgtgt aattgctgtt tgttcaattg ccatctcatg atcaccctgt tacgcataaa
    35761 taaatatgaa attataatca caaaatatgc ataaaaaatc actgaatggc aatcggaaat
    35821 cagagatgca ggaaattagc cagcagttga tgtccttgtt cgctaagaat actttctgga
    35881 tggaactgca caccttccag atcccactgg cgatggcgaa tccccataat ctcgcgggtt
    35941 tcgctccagg ccgtcacgtc aaagcacgct ggtaatgagt caggttccac caccagcgaa
    36001 tggtagcgtg tcacggtaag tggatttgcc agcccccgaa atacgccctc accgctatgt
    36061 gtaatcggcg aggttttgcc gtgcatgacc tttgcggcgc gcacaacttt gccgccaaat
    36121 gcctgcgcca ttgcctgatg accgaggcag acgccaagaa tcggcaagcg cccggcatag
    36181 tggcgaataa cgtcaaggga gatcccggct tcatctggcg tacaggggcc aggtgagatg
    36241 acaatttttt gtggtttaag ggcgtcgata tccgccagcg tcaacgcatc gttgcgctta
    36301 accagcacat ccgcccccag ttcacaaaag tactggtaga ggttccaggt aaaagaatcg
    36361 tagttatcta taagcaggat catggcggct ccggtacgaa agaaccgcgc tattttactc
    36421 agattccccg gcttcgctta ccactttgct aaatatcatc tgcagtgcgg tgagatcccc
    36481 cattgcccca ctctgatttg cctgattcca ggcctctttt tcgataccct gccagctcag
    36541 ttgatatccg gcatgaatcg ccagttgctc gaagaagatc cgctgtgcca gaccacttcc
    36601 cacccggaag ggatgcagca cgtcgatttc acaatagtaa tgcgccagcc gctcgacgaa
    36661 cttcgctttc tccaggccaa ccagataacc ttcttcctcc agatcctgca tcagggcatt
    36721 gccctctttt tcgatataag caaagtggca gaacggcgta tcgccctgat aaatatcaac
    36781 ttcacgcagt tgccctgccc agtcgaaaat atcctggtac agctggcgat ggatagtgcg
    36841 caaatgcggt aaaccgcgca ccagcggacc aagctcaatg gtcgcagcac gcagcgccgt
    36901 catttcgtaa gcggcctgtt ccagccgctg ctgctggcgg atgttcagcc ggttacgcat
    36961 gatatcaagg ccaggataaa gatacggatc gcgcccttcg ccgaatttat cgctcatagt
    37021 gcctcctcag ctcttcaagg cgcgctaaag cctctgcggc agtaagagtg actaaaggca
    37081 tctcgacgcc ttcaagacgg cgactggcct ggaaattacg attacgctga agctcccaga
    37141 gacgggactt ttgcttatcg gtgagtttct tcacttgatg cctccctgaa cgtgtccatt
    37201 tgccacaagt ataagcggca aatccgggtt acgccgggag agcagaagcg cgggcaagat
    37261 tgcccgcgcg agaaatcatt acggcaggac tttagcggaa aggataacta ccggttttga
    37321 cggcacattc tggtacggac caacatcatg agtcggcacc tgggaaatct tatcggcaac
    37381 gtccatgcct ttcaccactt taccaaatac cgcgtaaccg aaatcacgct gaccatggtc
    37441 aaggaaggcg ttatcggcaa cgttgataaa gaactggctg gtggcgctgt ctttgtcagc
    37501 ggtacgcgcc atcgcgatgg tgccacgcgt gttgcgcagg ccgttatcgg cttcattttt
    37561 gattggcggg tttggttttt tctgctgcat ctgctcggtg aaaccgccgc cctgaatcat
    37621 aaagccagga atgacgcggt gaaaggtagt gttgttataa aaaccgctgt tcacataatc
    37681 gacaaagttt tgcacagaca ctggcgcttt ttgtttatcc agctccagtt cgatgttacc
    37741 agctgaggtt gtcaacaata cgtgcgggtc ccctttcgct gccattgctg cgggagaaag
    37801 agcagaaaga gcgaaaacag cagccatcgc cgccagggtc gatttgaaca tgagatttcc
    37861 ttaacaaaca gagcagaaaa aaagcaagtg aagagattct aaatagcctt caagaacaag
    37921 gccatccctt tacctaattt tacgtatctg aaatatatgt aacctgacga tcacttttta
    37981 ttccgtgatc aaaatcacct cttaaaatgc aatttagcaa ccgattgcaa taaaacattt
    38041 aaacagatca caaaatcacc taaaatcgcc cgctcgcggc atatcccgct atgccatttt
    38101 atttttgaac gcaggccaat catgactaac agcaatcgca tcaagctcac atggattagc
    38161 tttctctcct acgcactgac tggtgcgttg gttattgtca ccgggatggt gatgggaaat
    38221 atcgccgatt atttcaatct gcctgtttcc agtatgagta acaccttcac cttcctcaac
    38281 gccggcattt taatctctat cttcctcaac gcctggctga tggaaatcgt cccgttgaaa
    38341 acgcagttac gttttggctt tctcctgatg gtgctggcgg ttgccggttt gatgttcagc
    38401 cacagcctgg cgctgttctc gacggcgatg ttcattctcg gggtggtcag cggcattact
    38461 atgtcgattg gtacattcct gataacacaa atgtatgaag ggcgtcagcg cggttcccgc
    38521 ctgttattta ctgactcctt cttcagtatg gctgggatga ttttcccaat gatcgccgcg
    38581 tttctactgg cgcgcagcat tgagtggtac tgggtttatg cctgcatcgg gctggtgtat
    38641 gtcgctattt ttattctgac cttcagctgt gagttcccgg cactgggcaa acgtgcacca
    38701 aaaacggatg ctccggtaga gaaagaaaag tgggggatcg gcgtactgtt tctctctgtt
    38761 gcggcgctgt gctacatcct cggtcagtta ggttttatct cctgggtgcc tgagtatgcc
    38821 aaaggcctgg gcatgagcct gaacgacgcg ggcacgctgg tgagtaactt ctggatgtca
    38881 tacatggtcg gcatgtgggc gttcagcttt attcttcgct tctttgattt gcaacgcatt
    38941 ctgaccgtac tggctggtct ggctgcgatt ctgatgtacg tctttaacac cggaacacca
    39001 gcacatatgg cgtggtcaat tctcgctctg ggcttcttct ccagcgcgat ctataccacc
    39061 atcatcaccc tgggttcaca gcagaccaaa gtaccgtcgc caaaactggt taactttgtc
    39121 ctgacctgcg gaaccatcgg tactatgttg acctttgtgg ttaccggccc gattgttgaa
    39181 catagcggtc cgcaggcggc actgcttaca gcaaacggtc tgtacgctgt cgtctttgtg
    39241 atgtgcttcc tgttaggttt cgtcagccgt caccgtcagc ataacaccct gacctctcat
    39301 taattgctaa tgccggacgg cactatcgtc gtccggcctt ttcctctctt cccccgctac
    39361 gtgcatctat ttctataaac ccgttcattt tgtccatttt ttgcacaaac atgaaatgtc
    39421 agacaattcc gtgacttaag aaaatttata caaatcagca acatacccct taaggagtat
    39481 ataaaggtga atttgattta catcaataag cggggttgct gaatcgttaa ggtaggcggt
    39541 aatagaaaag aaatcgaggc aaaaatgagc aaagtcagac tcgcaattat cggtaacggt
    39601 atggtcggcc atcgctttat cgaagatctt cttgataaat ctgatgcggc caactttgat
    39661 attaccgttt tctgtgaaga accgcgcatc gcttatgacc gcgtacacct ctcgtcttac
    39721 ttctctcacc acaccgccga agagctgtcg ctggtgcgcg aaggcttcta cgagaaacac
    39781 ggcatcaaag ttctggtcgg cgaacgcgct atcaccatca accgtcagga gaaggtgatt
    39841 cactccagcg ccggacgtac cgttttttat gacaagctga tcatggcaac cggttcctac
    39901 ccgtggatcc caccaatcaa aggttctgat actcaggact gctttgtcta tcgcaccatt
    39961 gaagacctca acgccattga atcctgcgcc cgtcgcagca aacgcggtgc cgttgttggt
    40021 ggcggcctgt taggtctgga agccgctggc gcgctgaaaa acttaggtat tgaaacccac
    40081 gttatcgaat ttgcccctat gctgatggcg gaacagcttg atcagatggg tggcgagcag
    40141 ctgcgtcgca aaatcgaaag tatgggcgtg cgcgttcact ccagcaaaaa cacccttgag
    40201 attgtgcagg aaggtgtcga agcgcgtaaa accatgcgtt ttgccgacgg cagcgaactg
    40261 gaagtcgact ttatcgtctt ctctaccggt atccgtccgc gcgataagct ggcaacccag
    40321 tgtggtctgg acgttgctcc gcgtgggggg attgtcatta atgattcctg ccagacttcc
    40381 gatccggata tctacgccat cggtgaatgc gcaagctgga acaaccgtgt atttggtctg
    40441 gtagcaccag gctacaaaat ggcgcaggtc gccgttgacc atattctggg tagcgaaaac
    40501 gcctttgaag gtgctgacct tagcgccaag ctgaaactgc tgggcgtaga cgtaggtggt
    40561 attggtgatg cgcacggtcg cacgcctggc gcacgtagct atgtttacct cgacgaaagt
    40621 aaagagatct acaaacgcct gattgtcagc gaagacaaca aaaccctgct cggtgcggta
    40681 ctggtgggcg ataccagcga ctacggcaac ctgctgcaac tggtgctgaa cgctatcgag
    40741 ctgccggaaa acccggattc tctgattctg ccggcgcact cgggtagcgg taagccgtct
    40801 atcggtgttg ataaactgcc ggacagcgcg caaatctgct cctgcttcga cgtcactaaa
    40861 ggtgatctga ttgctgccat caacaaaggc tgccacacag ttgcggcgct gaaagctgaa
    40921 accaaagcgg gtactggctg cggtggctgt atcccgctgg tcactcaggt actgaacgcg
    40981 gaactggcga aacagggcat cgaagttaac aacaacctgt gcgaacactt tgcttattcg
    41041 cgtcaggaac tgttccattt gatccgcgtt gaaggcatta aaaccttcga agaactgctg
    41101 gcgaaacacg gcaaaggcta cggttgtgaa gtttgtaaac caaccgtcgg ttcgctgctg
    41161 gcctcctgct ggaacgaata cattctgaag ccggaacata ctccgctgca ggattctaac
    41221 gacaacttcc tcgctaacat ccagaaagac ggcacctact cggtgatccc gcgttctccg
    41281 ggcggtgaaa tcaccccgga agggctgatg gcagtgggtc gtatcgcgcg taaatttaat
    41341 ctctacacca aaatcaccgg ctcccagcgc ctggcgatgt ttggcgcaca gaaagacgat
    41401 ctgccggaaa tctggcgtca gctgattgaa gctggcttcg aaaccggtca tgcctatgcg
    41461 aaagcactgc gtatggcgaa aacctgcgtg ggtagcacct ggtgccgcta cggtgttggc
    41521 gatagcgtcg gcctcggcgt ggaactggaa aaccgctaca aaggcatccg tacgccgcac
    41581 aaaatgaagt tcggtgtctc cggctgtacc cgtgaatgtt cagaagctca gggtaaagac
    41641 gtgggtatta tcgccactga aaaaggctgg aacctgtatg tttgcggtaa cggcggcatg
    41701 aaaccgcgtc atgcggatct gctggcagcg gatatcgatc gcgaaacgct gatcaaatat
    41761 ctcgaccgct tcatgatgtt ctacatccgt accgccgaca aactgacgcg taccgcgccg
    41821 tggttagaaa acctcgaagg cggcatcgat tacctgaaag cggtgatcat tgacgacaaa
    41881 ctggggctga acgctcatct ggaagaagag atggcgcgcc tgcgtgaagc ggtagtgtgt
    41941 gagtggactg aaacggtcaa tacaccgtcc gcgcagactc gcttcaaaca cttcatcaac
    42001 agcgacaagc gtgacccgaa cgtgcagatg gtgccagagc gcgaacagca ccgtccggca
    42061 acgccgtatg aacgtatccc ggtaactctg gtggaggaca acgcatgagc cagtggaaag
    42121 acatctgcaa aatcgatgac atcctgcctg aaaccggcgt ctgcgcgctg ttaggtgacg
    42181 agcaggtagc gattttccgc ccgtatcaca gcgaccaggt gtttgcgatc agcaacattg
    42241 atccgttctt cgagtccagc gtgctatcac gcgggctgat tgcggaacac cagggcgagc
    42301 tgtgggtcgc cagcccgctg aaaaaacagc gttttcgctt aagtgatggc ttgtgcatgg
    42361 aagacgaaca gttttccgtc aaacattacg aagcacgagt gaaagacggc gtggtgcagc
    42421 tgcgcggtta atgttttaac gggaggcgca atgcctcccc tttttgcatg gtcctgtaat
    42481 gaccttcggt atattgcagg acatttttta aactttttgt tttatttttt gtttttattt
    42541 tttaaaggat aatcaaatgt ttacagacac tattaataag tgtgcggcta atgctgcgcg
    42601 cattgcacgc ctgtcggcaa ataacccgct cggcttttgg gtcagctccg ccatggcggg
    42661 cgcatatgtg ggtcttggga tcatcctgat tttcacgctc ggtaatttgc tcgacccgtc
    42721 cgtacgtccg ctggtgatgg gcgcgacctt tggtatcgcc ttaacgctgg tgattatcgc
    42781 cggttctgaa ctgttcaccg gacacaccat gttcctcacc tttggggtaa aagcgggcag
    42841 catcagccac gggcaaatgt gggcaatcct gccgcaaacc tggctgggta acctggtcgg
    42901 ttccgtcttc gttgccatgc tctatagctg gggcggcggt agcctgctgc cggtagatac
    42961 cagcatcgtt cactccgtcg cgctggctaa aaccactgca ccggcaatgg tactgttctt
    43021 caaaggtgca ttgtgtaact ggctggtttg cctggcaatc tggatagcgc tgcgcactga
    43081 aggggcggcg aaatttatcg ctatctggtg gtgtctgctg gcatttatcg cgtccggcta
    43141 cgagcactct atcgctaaca tgacgctgtt cgcgctctcc tggttcggca accacagcga
    43201 agcctacacg ctggcgggta ttggtcataa cctgctgtgg gtgacgctgg gtaatacttt
    43261 atcaggtgcc gtattcatgg gattgggtta ttggtatgct acgccgaaag cgaatcgtcc
    43321 ggttgcggac aaatttaatc aaactgaaac ggctgccggt taattactaa ggggttttta
    43381 cgtggatcat ttgcctatat tttgccaatt acgcgatcgc gactgtctga ttgtcggcgg
    43441 tggtgatgtc gcggaacgca aagcaaggtt gctgttagac gcaggcgctc gcttaacggt
    43501 gaatgcatta gcgtttattc cacagttcac cgcatgggca gatgcaggca tgttaaccct
    43561 cgtcgaaggg ccatttgatg aaagccttct cgacacctgc tggctggcga ttgcagcgac
    43621 ggatgatgac acgcttaacc agcgcgtcag cgaagccgct gaagctcgtc gcatcttctg
    43681 taacgtagtc gatgcgccga aagccgccag ctttattatg ccgtcgatta ttgaccgctc
    43741 accgctaatg gtagcggtct cctctggcgg cacctctccg gttctggctc gcctgttgcg
    43801 cgaaaaactc gaatcactgc tgccgttgca tctgggccag gtggcgaaat atgccgggca
    43861 attacgcggg cgagtgaaac aacagttcgc cacgatgagt gagcgtcgcc gtttctggga
    43921 gaaattgttc gttaacgacc gcctggcgca gtcgctggca aacaacgatc agaaagccat
    43981 tactgaaacg accgaacagt taatcaacga accgctcgac catcgcggtg aagtggtgct
    44041 ggttggtgca ggtccgggcg atgccgggct gctgacgctg aaaggactgc aacaaattca
    44101 gcaggcagat gtggtggtct acgaccgtct ggtttctgac gatattatga atctggtacg
    44161 ccgcgatgct gatcgcgttt tcgtcggcaa acgcgcggga taccactgtg taccgcagga
    44221 agagattaac cagatcctgc tgcgggaagc gcaaaaaggc aaacgcgtgg tgcggctgaa
    44281 aggtggcgat ccgtttattt ttggccgtgg tggcgaagag ctggaaacac tgtgcaacgc
    44341 gggtattccg ttctcggtgg ttccgggtat taccgcagct tctggttgct ctgcctattc
    44401 gggtattcca ctcacgcatc gcgattatgc ccagagcgta cgcttaatta ccggacactt
    44461 aaaaaccggt ggcgagctgg actgggaaaa cctggcggca gaaaaacaga cgctggtgtt
    44521 ctatatgggg ttgaatcagg ccgcgactat tcagcaaaag ctgattgaac acggtatgcc
    44581 tggcgaaatg ccggtggcaa ttgtcgaaaa cggaacggca gtcacgcagc gcgtgattga
    44641 cggtacgctc acgcaactcg gtgaacttgc tcagcaaatg aacagtccat cgctaattat
    44701 tattggtcgg gttgttggcc tgcgcgataa actgaactgg ttctccaatc attaatttaa
    44761 cccgggccag agaattctgg ccttcttaat gactcttttt atatattcag caaataaaac
    44821 ataacccctt aataaatatt atctgctaat gggttatatt caccgttaca gcgacttaca
    44881 aaattaagtc attcgttcac tgacgccaat taaatattcc tgtatcgttc ttaacgagca
    44941 gtctgcttat attcatataa tcaatgaata ttaattaata tataatacat agggaatgta
    45001 aatgaacaaa tttattaaag ttgcactggt aggtgcagta ctggctacgt taactgcatg
    45061 tactggtcat attgaaaacc gtgataagaa ctgctcttac gactacctgc tgcacccggc
    45121 aatttctatt tctaaaatca ttggcggttg cggtcctact gcacagtaag tctctgacag
    45181 aaatcggcta acaccggttt aattgcccga cgccctctgg attttccgag ggcgtatttg
    45241 tttctatgtc cttcttgccc ccgcccgtaa aaatattttc actttaaatt caattttata
    45301 actacatcca ttagccacag cgcgatcctg tgcgaaattt tgtgatcttc ctccacatta
    45361 cacaacatca tatgttgtta tattcatcat gcattgtcat gttgcctttt aaatgactgc
    45421 aaactctccc ctacaacgta ttggacaaga aaaaggtatc gctatgggaa gccaggaact
    45481 ccaacgcaag ctcggatttt gggccgttct tgcaatcgcc gtcgggacaa ccgtcggctc
    45541 cggtattttt gtatctgtgg gtgaagtggc aaaagcagcg ggcacgccgt agcttacggt
    45601 gctcgcgttt gtcattggcg ggttaattgt gatcccgcaa atgtgcgtct atgcggaact
    45661 atccaccgct tatccggaaa atggcgcaga ttatgtttat ctgaaaaatg ccggaagccg
    45721 accgctggct ttcctctccg gctgggccag cttctgggcc aacgatgcgc cgtcattgtc
    45781 gattatggcg ctggcgattg tcagcaatct tggcttttta acgcctatcg atccgttgct
    45841 cggtaaattt atcgccgccg gattaattat cgcctttatg ttgctacacc tgcgctccgt
    45901 tgaaggcggc gcagcgtttc agacgctaat taccatcgcc aaaattatcc cgttcactat
    45961 cgtcattggc cttgggatct tctggtttaa agcggagaat tttgccgccc ttaccaccac
    46021 tgcgattggc gcaacgggca gctttatggc gctgctggcg gggatctctg ccaccagttg
    46081 gtcgtatacc ggcatggcct ctatctgtta tatgaccggc gaaattaaaa accccggaaa
    46141 aaccatgcca cgagcgctga ttggttcctg tctgctggtt ctggtgctct acaccctgct
    46201 ggcgctggtg atttccggcc tgatgccctt cgacaaactc gccaattctg aaacgccgat
    46261 ttccgacgcc ctgacctaga tccccgcact cggcagcacc gctgggatct ttgttgccat
    46321 cacggcgatg atcgtcattc ttggttcgct ttccagctgc gtgatgtacc agccgcggct
    46381 ggaatacgcg atggcgaaag acaacctgtt ctttaaatgc ttcggccatg tgcatccgaa
    46441 atacaacacg ccggatgtct ccatcatcct gcaaggggcg ctggggatct tcttcatctt
    46501 cgtttccgat ctcaccagcc tgctgggtta tttcaccctg gtgatgtgtt tcaaaaatac
    46561 cctcaccttc ggctccatca tctggtgtcg taaacgcgac gattacaaac cgctgtggcg
    46621 tactccggct ttcgggctga tgaccaccct cgccattgcg tcaagcctca ttctggtcgc
    46681 ctcaaccttt gtctgggcac cgattcccgg ccttatctgc gccgtcatcg ttattgctac
    46741 tggtctgcct gcttacgcct tctgggcgaa gcgtagccgc cagctcaacg ctttgtcgta
    46801 atgttatctg aagaaaataa aatgttggat attgataaaa gcaccgtgga ctttctggtc
    46861 accgaaaata tggttcagga agtggaaaaa gttctcagcc atgacgttcc gctggtgcac
    46921 gccatcgtgg aagagatggt gaagcgcgac attgatcgta tttatttcgt tgcctgcggc
    46981 tcgccactca acgcggcgca aacggcgaaa catctggcgg atcgcttttc cgatcttcag
    47041 gtctacgcca tttccggctg ggagttctgc gataacaccc cgtatcgcct cgacgatcgt
    47101 tgcgcggtga ttggcgtttc tgactacggt aaaacggaag aggtaatcaa agcgctggag
    47161 ctgggccggg cctgcggcgc actcactgcg gcgttcacca aacgcgcgga cagcccgatt
    47221 acctcggcgg cggaatttag cattgattat caggccgact gtatctggga aattcacctg
    47281 ctgctctgct acagcgtggt gctggagatg atcacccgcc tcgcgccgaa cgcggaaatc
    47341 ggcaagatca aaaacgatct caagcagttg ccgaatgcgc tcggtcatct ggtacgcacc
    47401 tgggaagaaa aaggccgcca gcttggtgaa ctggccagcc agtggccgat gatttatacc
    47461 gttgctgcgg gtccgctgcg tccgctgggt tacaaagaag gcattgtaac gctgatggaa
    47521 tttacctgga cgcacggctg cgtgattgag agcggagagt tccgccatgg cccgctggag
    47581 attgtcgaac cgggcgttcc gttcctgttc ctgctcggca atgatgaaag tcgccacacc
    47641 accgaacgcg ccattaactt tgttaaacag cgtactgaca acgtgatcgt catcgattac
    47701 gccgaaattt cgcaagggct gcacccgtgg ctggcaccgt tcctgatgtt cgtgccaatg
    47761 gagtggctct gctactacct gtctatttac aaagatcaca acccggatga acgccgctat
    47821 tacggtggtc tggtggaata ttaatccctc tcccggcccg gcaacgggcc ggatttacgc
    47881 aaggagttac ccgatgaaaa caggtatgtt tacctgcggc caccagcggc tgcctattga
    47941 acacgcattt cgtgatgcaa gcgagctggg ttacgacggc atcgaaattt ggggcggtcg
    48001 cccgcacgcg ttcgcgccgg acttaaaagc gggcggcatc aaacaaatca aggcgctggc
    48061 gcagacgtat cagatgccga ttatcggtta cacgccagaa accaacggct atccgtataa
    48121 catgatgctg ggcgatgaac atatgcgtcg tgaaagcctg gacatgatca agctggcgat
    48181 atatatggca aaagagatga acgcgggtta tacgctgatt tctgcggccc acgcgggcta
    48241 tctcacgccg cctaacgtta tctggggcgg ctggcagaga acttaagcga actgtgtgag
    48301 tacgcggaaa acatcggcat ggatctgatc ctcgaaccgt taacgccgta tgaatcgaac
    48361 gtcgtgtgta atgctaatga tgtgcttcat gcgctggcgc tggtgccttc gccgcgcctg
    48421 ttcagcatgg tcgacatttg cgcgccgtat gtccaggcgg aaccggtgat gagttatttc
    48481 gacaaactgg gcgataaatt acgtcatctg catattgtcg acagcgacgg ggccagcgac
    48541 acgcattaca ttcctggaga aggcaaaatg ccgctgcggg aactgatgcg cgatattatt
    48601 gatcggggct atgagggtta ctgtacggtg gagctggtga cgatgtatat gaacgagccc
    48661 agactctatg cccgccaggc gctggaacgc tttcgcgcgc tgctgccgga ggatgagaga
    48721 tgaaaaccct agcgacaatc ggcgataact gcgtcgatat ctacccgcaa ctgaataaag
    48781 cgttttctgg cggtaatgcg gtcaatgtgg cggtgtactg cactcgctac ggcatacagc
    48841 cgggatgcat tacctgggtg ggtgacgatg actacggcac aaagctgaag caggatctcg
    48901 cccgcatggg cgtcgatatc agccatgtcc atacgaaaca cggcgttacc gcacaaactc
    48961 aggtggaact gcacgacaat gatcgcgttt ttggcgacta caccgaaggc gtgatggccg
    49021 actttgccct gagtgaagag gattacgcct ggctggcgca gtatgacatt gtgcacgcgg
    49081 caatctgggg acatgcggaa gacgcattcc cacagctgca cgctgcgggc aaacttaccg
    49141 ctttcgactt ctccgacaag tgggacagcc cgctctggca gacactggtg ccgcatctcg
    49201 attttgcctt tgcctccgca ccgcaagaag acgaagcgct gcgtctgaag atgaaagcga
    49261 ttgttgcccg tggcgcagga acagtgattg tcacgctggg tgaaaacggc agcattgcct
    49321 gggatggcgc gcagttctgg cgtcaggctc ctgaaccggt gacggttatc gacaccatgg
    49381 gtgccggaga ttcgttcatt gccggattcc tttgcggctg gtctgcgggg atgacattac
    49441 cgcaggcgat ggcgcaggga acggcgtgcg cggcgaaaac cattcagtac cacggtgcct
    49501 ggtaggtata acgttggcgt gagcatcttc acgccaacgt gctgttactt gccggaaaac
    49561 gaccctataa tccgagtaat tcattcttta tttcagggtc gattatgtca gctacggacc
    49621 gctactctca tcaactcctc tacgctaccg tccgccagcg gctgctggat gatatcgcgc
    49681 agggggttta ccaggccggg caacagatcc ctaccgaaaa cgagctttgt acacaatata
    49741 acgtcagccg cattaccatt cgcaaagcca tcagcgactt agtggcagac ggcgtactga
    49801 tccgctggca gggaaaaggc acctttgtac aaagccagaa agttgaaaac gccctgctta
    49861 ctgtcagtgg ttttaccgat tttggcgtct cacaaggcaa ggcgacgaaa gagaaagtga
    49921 tcgaacagga acgggtcagc gccgcgccgt tttgcgaaaa gctgaacatc cccggaaaca
    49981 gcgaagtgtt ccatctctgc cgggtgatgt atctcgataa agagccgctg tttattgata
    50041 gttcatggat cccgctgtcg cgttatcctg actttgatga gatttacgtc gaaggaagct
    50101 ccacctatca gttatttcag gagcgttttg acacgcgagt ggtcagcgac aaaaagacca
    50161 tcgatatctt tgccgccacc cgcccgcagg caaaatggct gaaatgcgaa ctgggcgaac
    50221 cgttgtttcg catcagcaaa atcgcctttg atcagaatga caaaccggtg cacgtctccg
    50281 aactcttctg ccgcgccaat cgcatcacct taacgattga taataaaaga cattaaccgt
    50341 aggccggata agatgcgcca gcatcgcatc cggcgatgct ggcgcactga attttacatc
    50401 ccgcacgttc ccctcaccct aaccctctcc ccaaaggggc gaggggagcg ttcagtgcgg
    50461 aatttagctt gattgccgaa cggtgctatc cagcattaaa tactcgcaat actctcacgc
    50521 aaaatccgca gcaccgtctc ttcaccgctg cgattcgggt taatgcgaat cgcacaatgt
    50581 tcgagttgtg gattcgcctg gcgaaacgtt cctgaaagtc gatagaagag cggcgggatt
    50641 tcatatttcg actctgcacc gaccgggtaa ggcaacgcgc cacgcttttg cgcttcttcc
    50701 agcaccctgg cggcaatcag ctgatgaaac tcgacaatca acaccttcga ctgcgcattg
    50761 gcaatgacgg cgcttttcac ttccgccacc gcgccgccgt taagcaaagc cagcaaccgt
    50821 tcggatactc ccgcctgcac cgcatgcatc actggcgcga acaccagacc acgcaagact
    50881 tccagcgcct gtgagccctg gatctggcta ccgccggagt aaagcgtggc gcgaatccga
    50941 ctaataacat cggcatcgcc aaccactgca ccaacgccct ccggcccaaa cagcttgaag
    51001 caggagaatg tcgagacatt cgcgccgcat tcacagccga ttcgcgctac cttcatcacc
    51061 gcatagttat catcggttaa cgctggaaca cctgccgcgc gcaacgttgc cagcacatct
    51121 gccagcacat agctgtcctg cggctgctgg cgcgtatgct gcaccagcgc ggcatcaggt
    51181 tgttgctcgt cgacgacctg cttcagtgcc aacaggtcat tgaaatcagc agtaataagc
    51241 gtcagcccca tctgctcaat aataacccgt gtcgtcgggt aaacaggcgc gtcatgcacc
    51301 agaagacgct gccccggttt gagcaaagcc gccagcccgg cgcgaatcgc gccagtcccc
    51361 gcgccctgca ccagcgccgc ggcctgtgcg tgaaatgcat cagccagcac ctgctccacc
    51421 cgttgggtaa tacgcggttg attcagacct ggagttaacc ctaaatcacc gccagcaaga
    51481 aactcgctgc ctggaaaatg gcggcaaata gtgtccacca gcgcaaactg cttttgctgc
    51541 gcttcagcaa gagtcaggct ttgcaaagga aacgtcttca taggagcctt ccttaagccg
    51601 gaacgaacag gccaagccag taaagaacat tcagcagaat accggtgatc attactgcca
    51661 cgaccggtgc cgccattttc tgtaccggac ggcccaggga ttcgttgagg aagtaaatgg
    51721 caaccgcgat agagaatccg gtataacccg ccatcttaat tgctgcgaaa atcgaaccga
    51781 ccagcagcgc cacttccatc agcatattca tggcgttacg gatgttatcc gacgcattac
    51841 gcaccgacgg gtagcgtccc agccatttgc cgatcgaacg aagcagcaag acttccgccg
    51901 aaataaccac tgcgcctaat accgctgcaa ccatcggatt cggcgagaga tagcccaccg
    51961 cataaacaaa ggtaaagccc gcaactgcat acacacccgt tgctaaggcg gtggtggcaa
    52021 tcaacggcac aaaccccagt ccgcgcataa attctgccag agccgcctga ttaatcagcg
    52081 tttgcgattg ttccggcgtc acgcctgcgg aatacgcttt ctccagtgtg aagatcgaaa
    52141 cttcgctacc agcaaaaatc ttcatactgg caacggcggc aatcaatgct cccacgatgg
    52201 cgatataagg taagtttttg ataatccgcg acgtgcgctc ttcaaacacc gaaagcccgc
    52261 tggcatcgat gtcattttca tcacgatgac gcaggtcgtg agttatcgcg atccccagca
    52321 gcatcaccat gccaataaag atttcaatgg attcagggtt aagatgtggg aaatagcgca
    52381 cgactaccac acgggtcatc agaacaacca cggcggcgac cagactttgc ttccagccaa
    52441 actggtagaa aatcgccacc agcgggaaca gtgcaaaagc agaaaccacc ggcgagctta
    52501 attccccgag gctacctaat acatccaccg gcagcgcggt cagcagctgg ttaactggca
    52561 acaggcaagt aaggatcaac acgccccaga tagcgccaag accaaacgcc atcaggctgt
    52621 ttatcgccag tacgccgagg atatcggtag gaagaaagag taaccatgcg ttgagcagcc
    52681 aggttttcag ggtaaaagag atccccaccg aggccacaaa accaatgctc agaccaaaag
    52741 cgatactccc cgcctcgcga cggttcatat agccttcaat cagttgcggc aggatcgggt
    52801 ggatgccgtc atgaaaaaca gccgccgagc gatgcgccag aagcgatgtc atacccgtca
    52861 ggcacgccac cacgataatc tgaatataca gatccatagt cgtgcctttt atttcagatg
    52921 gttaatcaac atcggaatcg cgtgttcgac gtactcaacg gaaaggccaa acgccacttt
    52981 cccttcagcg atcattttgg cgatatgctc gtctttcgct ttaatgcctg gtttggcaat
    53041 ggtgcaactt ttgttatagc caatcaccgc gatggcaatc gacaatgccg cgcccgcacc
    53101 ggtattacac gcgccaatgt aataatctag ttgcccggat ttcaccttca ttgccgcttc
    53161 catgtcgttg tgaatgaaaa cttcaaaaca gccaggagcc gtcgcttcaa tggttttttt
    53221 aatctgctca cgctgtaagc ctgcaacgcc aatctttttc atgagtctgt ccttattgga
    53281 aaaattgaga gggattttca cgtaacatca catccacatc ggcctgactg aatcctgact
    53341 ggcacaattg cggaataaag gtggtcaata aatagtcata gccataacca ccattggctt
    53401 ttaaatggga gcggcgcgta atatccatcg acagcatgac gcggttcagc aacccacggt
    53461 cacgtagcgc gtggagcatc gcaatgcgct tttcgtccgg gtagtaactg ttcttaccaa
    53521 tggtgtcgaa ctgcacgtac gcgccgagat cgatcatctt caaaatgttg tcgaggttgt
    53581 ctttcagatc gcagtgacca acggtgacgc gcgaaagatc aactccgtgg gcttgcaaca
    53641 gagccagttg ttccagcccc atggtgctga acgacgtatg cgtggagatc gggcgtccgg
    53701 tctggttatg cgccagcgca gcggcaataa ataccttctc ttccagcggc gtaatctttc
    53761 cttcgctgga gccgatctcc gcgatgatcc cggctttcag ctccgtgcca tcgataccct
    53821 gttcaatttc atcgaccatc tcctgcacca gttcctgcac gctgcgggtc accacatgtt
    53881 ccgggaaaaa cgcatcctgg taataaccag tacaggccac cacgttgatc ccggtttcgc
    53941 gcattacatc gagcataaat tgcgcattgc gccccatgta acggttggtc atctcaatca
    54001 cattacgtac accccgggtc atcaggtcgt tcatctcctg gcaaatgaac gcatactgat
    54061 caaggcggca gtccacgttg tttttaaagc cggagagatc aatatgcaga tgctcatggg
    54121 ccagggtgta acccgtcgga tcaaaactca tagggtgtct cctgcaaacc ggagggagga
    54181 aagaaaagag cgaccatttt gtggcggtgg cgcacggaaa aattcacaca cggtagctcc
    54241 cacatcagaa agcgtggtgc gcacaccgag ctgcgtggcg accagccctt gctgataaac
    54301 cagcactggc accacttcac gggtatggtg gctgtgacca atggtcggat cgttaccgtg
    54361 atccgccatc acgaccaggc aatcatctgg ctgcatcgcc tcaacaagcc gggcaaggtt
    54421 acggtcaacg acctgcaaac gttcggcata acgtgcgacg tcttctgcat gaccagcgag
    54481 gtcggtttcc tggatattga tgcaaataaa cgccgtcgga tgggtgttaa attcgtcgag
    54541 ggtgatatcc ataatccgct ggctatccac caggttttgc cagctcacac cataaggatt
    54601 gctgacgata tctgccacct tacccaccag cacggttggc acgcccgctt catacagttt
    54661 ttgcggcacc tgcacttttt catcaacacc gtagcccata tgcacgacct ggaagccgtt
    54721 gtcataagcg ccagaacgcg gcgcattaat accaataaag cgcccttctt tgctttctgc
    54781 ggcatcgaga atacgctgac tgttggttaa caggccgcca aatgtaatga cccgaccgac
    54841 ctgtacctgg tcacgcacga tacgaccaat tttgattgcg tcgtcaaaag agatcacaga
    54901 gagattggcg gtaatgttat agacttggcc taaatccgcc tcgagattat cgccaatcgc
    54961 aaccgcctga ttgacccaca gaaattgcag atcatcgcca cggcgctcca cctgccagcc
    55021 agcggaaact aatgcctgct caacacggtc aatcacatcg cgaaaaggca tccgcagcgg
    55081 cggtaacggg cgcgtgccta aaatttcctg atgccccata aaggtatcgc caccttcatg
    55141 ttgcagctct gccacgcccc aggttgcgga atctgacggc tgcatatcgc ctggcgcata
    55201 acccaatgtg ttgattagcc ccagcttctc caacgttggt agctgcaaat gcggcaactg
    55261 gctcaggatg tgaccacagg tattcgcccc ggcatcttgc ggacgcacca gcgtgacatc
    55321 tttcattgcc cctacgccaa agctatcaat cactaacacc acaaatcgcg ccatttatgc
    55381 cttcagaggg ttgccgaggc tgtcataacg accaactatt tctggttcgc cgcgctgaat
    55441 accggacacc agcaccacat cgctacgggt gacaaaaatc tgcgtgcgaa aacagagcac
    55501 cactgcactg ctcaccggat actcgcctgc cagcggcagg gtgtagtcga tactgctgtc
    55561 atccacagtt ttgagattgg tttcagtaat cttttgattt tctggcgtaa acaccagcgc
    55621 atgttgcgca tgaccacgac ggtagtaacc gccgccgtag cagtagctgt cgccacggaa
    55681 atgatgggag atttcactta accagagcat cgcgatacgt tcaggctgat cgccctgctg
    55741 gtttgccgga atagtaccgg tcagcgcatg accgggttcg gcatgagtcg caccgtattc
    55801 cgccagcaat ggcagcgaag tgcagctggt cgctgaaggt acgttcagtt gctcaatcgc
    55861 aataccagat ttcgccagtt gatcccgtgc ttgtaccagc gtgtgaagat tcggtgttgg
    55921 caaaactttc ccggcagcct catcccaaag caggcaaggg aaatgggtta gtccggctaa
    55981 atgcagcccc ggcagtttct taatttcagc aatcacctcg tgcagcgaat gctgtacaaa
    56041 accgctctcc tgccccggat aaagaaaatc atcgtcgcta taaactttga gcagcacagg
    56101 ctggacccgt ccggccttaa ccgctgccgc agaaacttcc cgcgctttgt cgagagtaaa
    56161 cacggtgatg atgtcggtgc cctgttcaac ggcgtcagca acctgatgac aagggatttg
    56221 taccagatgc ccctgatgcg ccactggcaa accagcgcgg cgcatgactc gcgcctcttt
    56281 gtaatccacc gccacaatgc cgctgtagcc taatgccagc aatttttccg ccagccacgg
    56341 attacgacca aattgcttgg tcatcagata cagttcaatg ccgtaaagcc gcgccgtctc
    56401 aatgagccgc ttaccgtttt ccagtatctg atccacgtcg atcacccagc tgtccggggc
    56461 gattttgccc tgctgccaca aacttagtgc ggcggaaatc agcgccgggt tctggcgttt
    56521 caatgcttct acaaacatct tgttgcctcg tttctgttcc ttgaattaaa tattcatttt
    56581 tttaaatatt taggttgtgc gacgtggatt tgtcgggagc ttcaaacctc ttcgttagca
    56641 gccatccata atccataaag attggccaga aaatagcctt cttcacacgg gttaacttcc
    56701 agagcgaatt ccttcaacaa cacctgatgc aattgcacaa cggcttgcca gtggctggat
    56761 tgcgccagtt ccgccagcaa ctcgttatcc agcggctcta tttcttcacc acggcgactg
    56821 cgcatcagtg cactcgccat atgtgtcatc gccatcgttc cttgctcact gcgcaccggc
    56881 agatggcact ctttttccag tacgttgacg acctgcatca tgcctttgca gacgtcctta
    56941 tcaataacgc ctgcctcgca aagcaggttg agtctggttt ccatatcaga tgcctttcgt
    57001 ttaataactg ggttcctgtt ccccactcac cacccgttgt tgatgggcaa gcagggcgtg
    57061 tttatctaca acggcacgca gcaattgttg cattgggtaa tcatcgactc gcgtcaggac
    57121 cacggcttcg gtggccagta aaaatcgcgg atcgtctgtt agcggcgtcg cctctaatcc
    57181 cagcatggtc agttcgtttt ccgccaccac gttccagatc accgcatcga catcgccttt
    57241 aacaatgcgt tgtaaactct cgtgataaga gagatcgact cgttccacat cactatcgcc
    57301 aaaaaagaca tcggtcatga ttttctgatc cgccgaacga ctatcgagac ctactcgctt
    57361 aacatttcca gactcacctt tgcggcatat cagttgatgt tcaccaacat acgtatgcgg
    57421 gccaagctcg agggcgatgc acaaattgtt ttggctgaga taactttccg ccgccaggcg
    57481 ggaaactact gccatgtcat agacaccgtt aagtaaacac tccacgcgaa tatccgcgcc
    57541 gcgcatatgc gcatagtaaa aaggaatgcc atcaaactgg gctttcaatc cgctcgctaa
    57601 accttcgtac aagcgggtat agggcaaagg catcgcacat accacgttgt tgatatccac
    57661 atgactcagc aatgctttgt tatccatctc gacaagataa ctgccattgc gcccacggcg
    57721 ttcaatccgt atcgctccgc ttgattccag cgttttcaac gcggcctgcg tcaggccaac
    57781 cgatgaacgg cattcagttg ccagctcatc tatggttttc agtcgattac cgcatttttc
    57841 acccaacaaa taacgggcca gtgtcgtgat gacgacgcct tcttttttga taaacgttcg
    57901 acgcataata tattttcaat aaaatgaata tttatatctt cattaaaatg aagataagtg
    57961 gcgacgacga gatgtggaaa cggcgaggcg cttcacattt tttcgggttg ttgcgaaaag
    58021 aggataaaaa aaccgggttt ccccggtttc agagtgatga cgaagtgatt tattcgacaa
    58081 tgcgagcaaa gggtaattcc cactggcgct tgcctgctcc cctcacccta accctctccc
    58141 caaaggggcg aggggaccgt ccgagcgttt tgatattacg gcttcgccac aaaaccaatc
    58201 gcttcgtaca ccgctttcag cgtacgggaa gcgtgtacac tggctttttc tgcgccgtct
    58261 ttcatcacct gttgcaggaa ggcttcatcg ttgcggaaac ggtgatagcg ttcctgcaat
    58321 tcagtcagca taccggaaac ggcatcagcc acttcacctt tcagatgacc atacatcttg
    58381 ccttcgaact gtttttccag ttctgggatg ctctggcccg ttaccgcaga aaggatatcc
    58441 agcaggttgg aaacgcccgc tttgttctgc acatcgtagc gaactaccgg cggctcgtcg
    58501 gagtcagtga ccgcgcgttt gattttcttc actaccgatt tcggatcttc cagcaggccg
    58561 ataacgttat tgcggttatc gtcagacttg gacatcttct tggtcggctc cagcagcgac
    58621 attacgcgcg cgccagattt cggaataaac ggctccggca ccttaaagat ctcgccatac
    58681 agcgcgttga aacgctgggc gatatcgcga ctcagttcca ggtgctgttt ctggtcttca
    58741 cccaccggta ccagattagt ttgatacagc aggatgtccg cagccatcag caccggatag
    58801 tcaaacagac cagcgttgat gttctcggca taacgcgcag atttatcttt aaactgcgtc
    58861 atgcgactca gttcgccgaa gtaggtatag cagttcagtg cccagcctaa ctgcgcatgt
    58921 tctggtacgt gggactgaac aaaaatggtg cttttctcag gatcgatacc acaagccaga
    58981 tacaaggcca gcgtatccaa cgtcgcttta cgcagcttct gtgcatcctg gcgcacggtg
    59041 atcgcgtgtt ggtcaacgat acagtaaatg caatggtagt catcctgcat gtttacccac
    59101 tgacgcagcg cacccatgta gttaccaatg gtcaattcac ctgagggctg tgcgccacta
    59161 aaaacgatgg gcttagtcat ttttcgattc ctgattttcg ctatgcggaa gccctaatgc
    59221 gggcagaagg tcatttatag actgataaat tacatcaggc tggctgagat cgatagcctc
    59281 gccgtagtta tatccgtagg ttaagccaac tgatgggcaa cctgccgctt ttgccgcctg
    59341 aatatcattg cgtgagtcgc cgacaaacag catctgttgt ggggcaattc ccatccgctc
    59401 agccaccagt aacagcgggt ccggatgcgg ttttttgttt tgcacatcat cgccgccgat
    59461 aaccacgcta aagtatttgg caatatctaa ggcttcgagc agcggcgcga cgaacggcgt
    59521 cggtttgttg gtgaccaggc ctagcggcag gcctttagcc tgcaacgcgc ccaacgtatc
    59581 ggcaacgtgc gggaacaaaa acgtcccctc ttcggcaacc tcgccatagt agcgatcgaa
    59641 cagtttacgc agaatacgta cctgttcttc tgccggaatg tcgtcatcaa cgggcggttt
    59701 ccccattgtt ttacgcagag tcgcacgttc ctgacgcgcc caggtcaatg cgcgctccat
    59761 cagaacatct gcgccgttac caatccaggt aataacgcgt tcttcacctg cgacgggcaa
    59821 ctccagcgca tacagcgcca tatctaccgc agcagcaaga ccaggagcac tgtcgaccag
    59881 cgtaccatca agatcaaaag cgacgccgcg aatatcttca aacttattca tgacttacct
    59941 ttgccagttc actgcgcatt tcatcaatga cttttttata gtctggctgg tcgaagattg
    60001 ccgaaccggc gacgaacata tccgcgcccg ccgcagcgat ttcgccaatg ttgttcacct
    60061 tcacgccacc gtccacttcc agacgaatgt caaagccaga ctcgtcgata cggcgacgta
    60121 cttcgcgcag tttatccagt gtttgaggaa tgaaagactg accgccgaaa ccagggttga
    60181 cggacatcag caggatcaca tccagcttat ccatcacgta atccagatag ctcagaggtg
    60241 tcgccgggtt aaataccaga cccgctttac agccattttc tttaatcagt tgcagcgtgc
    60301 ggtcaacatg ctcggaggct tctggatgaa aggtaatgat gctggcacca gcggcagcga
    60361 aatcaggcac aatgcgatcg acgggtttca ccatcagatg tacgtcgata ggggcggtaa
    60421 tgccgtagtt acgcaaggat ttcagcacca ttggcccaat cgtcagattg ggaacatagt
    60481 ggttatccat gacgtcaaaa tgcacgacat cagcgccagc tgccagggct tttgcggtat
    60541 cttcacccag gcgggcaaaa tcagccgaca gaattgaggg ggcaatcaaa tactgtttca
    60601 tccgcttctc cttgagaatt attttttcgc gggtgaaacg actcctggtt tgtacaaagc
    60661 cagcagttcg tccacctttt tacgtgtgcc gccgttgctg cttatactgc gtcgaacttt
    60721 gacgacatgc aattttgcgc gctgatacca ctcacgcgtt aacatcgtat cgtgattgga
    60781 gatcagcact ggaatatggc gctcaaccag accttcggcg atctccgcca gatgcgcttg
    60841 ttgttcaagc gtaaaactgt ttgtgtgata cgccgtaaag ttggcggtcg cagacagcgg
    60901 tgcataaggc ggatcgcaat agacgacgga tgcatcatct gcgcgctcca tgctatcggc
    60961 gtaagactca caatagaaaa aggcattctg cgctttttca gcgaagtgat acaactctgc
    61021 ttccgggaaa tagggttttt tgtagcggcc aaacggcacg ttaaactcac cgcgcagatt
    61081 gtaacgacac aggccgttgt aaccgtagcg gttcaaatat aaaaacagta ccgcccgacg
    61141 gaacggatcc tggcttttgt tgaactcttc gcggaactga tagtaaacct cggcgcaatt
    61201 tgtttcggga acaaacagct cgcgtgcggc ctgtacgtac tcatcagtac gcatcttcac
    61261 aatgttatag agactgatca ggtcgctatt gatatcggca aggatataac gagaaaagtc
    61321 ggtgttgaga aacaccgacc cggcacctac aaaaggctca accagacatt cgcccttggg
    61381 caaatgccgt ttaatatcat caagcagggg atacttgccc cctgcccact tcaaaaaagc
    61441 gcgatttttc ttcatgccga ctaactaatt acaccttctc cggctgtgga gaaagctccg
    61501 acagcatcca gcgcttcagg cagctcccgc aacgataaac cattgcggga gataaccttg
    61561 attacttcag atcggcctgt acctgacgca gcggtttcgc ccacgggttt ttggcctgaa
    61621 catctgctgg caatgtagat accgcttttt tcgcctcttc tttcgaagcg tacacgccag
    61681 aaaccaggac ataccacggc tgaccattac gcgtcgtttc atagacaacg tagtttttca
    61741 gattctcttt cttcgcccaa ccgttcaggt tgtcgtagtt agaggaactg ctcagctgca
    61801 gagtgtaatg gctggacggt gccgatttca acgaaccaac attacctgcg gtcttccctc
    61861 cagcggctgg tgttgccgtg gtttgcgccg gggatgccgt ctgtactgga gccgtagtcg
    61921 ccgtctcttt tggtgctggc gtagaagtcg cagccggtgc cttcgtgctc gccacagggg
    61981 cagctggttc agtacgcttc ggcgtctgtg ctaccggctt cggctccgtt ttcacggttg
    62041 cttgcggttt tttcggttca atcaccgcct gctgacgaac tgggcgcgta gtggacggac
    62101 gttcagcggt ttgcgttttc gccgtgtcac gcgatgcatt gccattgcga acaggcgcaa
    62161 ccgttgcggg ttcagtcggc aacgtggaat tgaccgccac attgttcagc tgttgctgat
    62221 tttgtggctg ggtcagcgca ttgttcaggt caccctgcac ttcaacacgt tgttgaccat
    62281 ccgttgccac cggggtttgc ccttgagtcg gcgtagaaga gatcggtggc agagaaacat
    62341 cctgctgagt attttccgca gacgtggttc ccggcgctgg ctgcacgcca ttcgcctgat
    62401 cggtcgcatt gccagcaaga tcaatactct tctcgccaga cgcggtttga tcgctggaag
    62461 tggtcgaggg ggcttttagc gcagaaccga taccgatgat caacagcagt agaaccagaa
    62521 tgccgacgcc catcatcata tactgacgag aagcgggttt actggctgct tttttgcgct
    62581 tacgcggacg acgctctacg cgctcttcat ccacggtttc atcttcggat tcgtcaattt
    62641 cttcttcgat ttccggttcc tcattgcgct ctttttgcgc acgagtcgga cggcgatcgt
    62701 cagtgtcatc aagttcaata tcatcaaaat tgatctgcgg ttcgccacgt tcagtacgct
    62761 cagaacgttc agaagattga cgagaacgac cagtacgacg atcgctggga tcgggtttca
    62821 gctcgtcttc tggtttgaat tcatccattt aacaccccac taaaaggtta atgcttacca
    62881 cgttgcaatt aacctgaagc taatagaccg cttgataagc ggcctgacct ttcttgttgt
    62941 tacgctgatt gacaatcggc aatggcgtta agaacaagct cgtgcgaaac gccgctgcga
    63001 acttcactct taccaattgc caacggaaga attaagcgca tctctcccgc aaggactttc
    63061 ttgtcacgca gcatatgcgg taaatacgcc tgcgcggaca tttcgcgcgg cccattgacc
    63121 ggtaacccag cccgcgtgag cagggttata atacgctgcg tttcggcaga actaaactgc
    63181 ccgagacgtt ccgacgtccg cgccgccatc accatacccg cagcgaccgc ttcaccatgt
    63241 aaccaattgc cataccccat ttcagcttca atggcatgac caaaggtgtg tcccagattc
    63301 agtaaagcac gtaacccggt ttcgcgctcg tcggcggcga caacttctgc cttcagttca
    63361 caacaacggc gaatacagta cgccattgcc ggaccgtcca gacgcaacaa cgcatccaga
    63421 ttctcttcca gccagttaaa aaacgcaccg tcaagaataa tgccgtattt gatgacttct
    63481 gccagccccg acgctaactc acgcggggga agcgttttca gacagtcgag atccaccacc
    63541 actgaagcag gctggtagaa cgcgccaatc atgtttttac cgaggggatg gttgaccgca
    63601 gttttgccgc caacggagga atcgacctgc gacagtaacg tcgtcgggac ttgaatgaaa
    63661 cgaacaccgc gctgataact cgccgccgcg aagccggtca gatcgcccac tacgccgccg
    63721 ccaagcgcca ccagcgtagt atcgcgacca tgcggctttt gtaacaacgc cgtaaagacg
    63781 gtatcgagta cagctaggct tttatactgc tcgccgtcag ggaggataac gctatcgacg
    63841 ttaacacccg cctgttcaag tacgccgcgg accttatcga gatacagagg agccagggtt
    63901 tcgctggtga ccaacatgac ctgctcgccc gatttcagcg gtaagaatga agctggttca
    63961 ttaaacaaac cagatgcgat ggtaattggg taactacgtt ccccgagagt aacgacaatc
    64021 ctctccataa cgcgacatcc accttaatta ctgtacccgc agacgagtgt atataaagcc
    64081 agaattagtt gctttccagc atgtgaataa tctggtttgc aaccacttta gcgctttgat
    64141 catcagtacg aatggtcacg tcggcaatct cttcatacag cggattgcgt tcattggcca
    64201 acgcttccag aacttcacgc ggcggtgttt caacgtgcag caacgggcgt tttttatcac
    64261 gctgcgtgcg tgcaagttgc ttttcgatgg tcgtttcaag ataaacgaca acgccacgag
    64321 cggaaagacg gttacgcgtt tcacgggatt tcacagagcc gccgccagta gccagcacaa
    64381 taccctgttt ctcggtcaac tcattgatga ccttttcttc gcgatcgcgg aagccttctt
    64441 cgccttctaa atcgaaaacc cagcccacat cagctccggt tcgtttctca atctcttgat
    64501 cggaatcgta aaattccata ttgagttttt gagctaactg gcgcccaata gtgctttttc
    64561 cggcacccat aggcccaacc agaaagatat tgcgtttctc tgccattttt tcggtactac
    64621 taagactatt cgttaatgat aaacccgctt cgctcagaga gcgccgcagg acatgaactg
    64681 aaacctcata agatattgcg agagtcagac tgaaaattat ctcaatactc cagcgggttt
    64741 ggcaactgaa taaatcaccc atccctctgc atatctggtc gctgcaagcg cactgccttg
    64801 ctaccaccgc tctggcgata aatcaccggg taagattagc gtaaaaaaga cagcaaaatg
    64861 ccgcctgaat gataaatcat catcatggaa cgcaggcagc gaagagcacc aactctcaaa
    64921 tcggtactcc ttgtatgcta aatacctgcg cgcgtcaaat agatgaaaca cgttcagcat
    64981 aaaaacatta ccgcttttac ggctgtttac tcactggaaa ccagtcgtgg cgtgataaac
    65041 accactaact cgcgtcgttc atcttctttt ccgtcatgac gaaataattg cccgaaccag
    65101 ggaatgtcgc caagcaacgg tacgctatcc tgacccgatt tatttttacg ggtaaaaatg
    65161 ccgcccagcg ccaacgtttc tccgcttttt acctcgacct gcgtttcgat ctcctgctta
    65221 tcaatcgcca gcacttcgcc atcggcctgc tgtagcacct gccccggaac gttctggctg
    65281 atgtgtaatt tcagccggat gcgacctttt tgtaacaccg tgggcgtgac ctccatcccc
    65341 aggacggcct ctttaaattc caccgacgtc gcgccacttt ccccgctgga agcctgatat
    65401 ggaatttcgc tcccctgttt aatgctggca ggctgaagat gtgaggccag cagacgcgga
    65461 ctggcgataa tatccagctg ctgtttttgt tcgagcgcgg aaagctcaag atccagcaag
    65521 cgtccgttga tgcgcccaat gttaaaaccg acatgcgttg tcgccgtcgc tacggagagg
    65581 tcgctaccaa gcgtggtgac ttgcccaacg ccaccagcgt gttgcgcatc ggccagcgtc
    65641 catttcacgc ctaactcacg caaacttttt tcattaatgg tgacaatatg cgccgacagc
    65701 tcaacctgcc cgaccggcag atccatttgc gctacccact gttcaagcgc gcttaacgcc
    65761 gttttgttat ctcgtagcaa aaggcgattg gtgcgtttat cgacggtcat actccctttg
    65821 gcactcagta gcttctcccc cgctttcgcc agttctcccg cgtcggcgta ttgcagggtt
    65881 atactgcgat tttccagcgg cagatttgcc tgcgcccgcg cctgctccgc ctcctggcgg
    65941 gcgatattgt cattctgcca ggcaatggaa tgcactgaga gaatgttgcc ttcctgccgc
    66001 gttatcagtc cggcgctttt cactacagtt tgtagtgcct gcttccaggg aacatctgtt
    66061 agatgtaacg acaccgtacc gctgacgtct ggcgacacga ccaggttcaa cttctcctgt
    66121 tcagccagcg cctgcaacac ctgagctacc ggaacgtcat ccaccatcag cgtcactttt
    66181 tgcggctttg ccgcctgtac gccgggtatc agcatcaaca gtagtgcggc tatccattgc
    66241 ttcatttgta tctccttgcc gttgccacaa ccattgtggc ggttcgcagt ttgtcccggt
    66301 acccagcgtt agtacgtctg gcgtcagctg taaaattgtc cagccgtttt ccagcacatc
    66361 gttttgctgc acccgtcgcc atttcttttg cccgtctttt attacaccga tgatgcgctc
    66421 gcctcgccct accatcccct gatagcgcca ctggctaagt tcgctaatcc ggcatagatc
    66481 ttccggcgat ttaaaagggt cacgcatacc ggttaaaagg cacaatgcaa tacctgccaa
    66541 caaccagcgt ttaaccctca tgcggcatct ccagttgtag cgtgaacaaa agatcatcac
    66601 cttccacgct taacgaaaaa cggctcacgc tgacgttgcg ctctgccagc cgtgtaaatg
    66661 ccgacggcac tgcttcccac agcgttttca acgccaactc gcctccctgc gcggatggat
    66721 gccaggaaac cagttgcgcg ccggataact gaaaatccag tggcgaaaag ggcagcgttt
    66781 tttcctcgct gaagggagtg gtgtctacca ggcgatacag tgcggcccac tgctggtgat
    66841 gacttgcccg tagtcgaatt aatgcgtcgc gctcttcatg gtgtgtcgat gatagaaaaa
    66901 tgagcgtaac taacatcagc aaccagaatg cccagcaaaa ctggcggagg cggggtgatg
    66961 tggcgaacca ccagtcaaag aacatgttca tcgctaacct tccttgttaa ctgatactca
    67021 aattgccagc gtccctgcgc atcctgctgc gtggctcccc gctgattgag atgaaaagac
    67081 gcatcctggc ggagtgacgt ttctagtgcg tttaacgcgg taatgcttgt tgtcagcccc
    67141 ttgatctcca gcgttccctg ctgccagctt atcgttgtca gccaggcgtg ctcgggtaaa
    67201 agcgccgcca gtgcttccag cgcagattgc cagtcgcggg taaattgtcg ctggcgctgc
    67261 cgttgcgaac gctgttcgcg taactgctgt tgctccagca aacgtggctt cgttatctgt
    67321 aagctgcggg cgagttgttg ttccgcctga agcaaaactg cgtctatgcg cgcttcggcg
    67381 ctgcctgtca gacgcagtat tagcgttatc ccgacggcca gcagcagagg cgcaacgaac
    67441 atcagcaacc agaaacgcag aaaagcggtc cggcgttgct gtcgccaggg caaaaaatta
    67501 attggcgggt tcatcagtac gccttcccaa gcgccagccc cagcgcgatg gcaaagtctc
    67561 caccaggcgg cggtagcggc ggctgacgaa cagaaacggc ctcccagggg tcaaatccgc
    67621 cttcgccaca tatcgcgacg ctgtcgggat caacggataa cgctgccgcc agctccttcg
    67681 cgctagtcat ccccaccgcc agtttgcgcc cccagctata gcgcgtcgcc cacagccact
    67741 gttcgttatc acgccaggcc agacattgct gatgagaagg taaaaaaggc aggaatcgct
    67801 gtaatgcact ggcatccggg gtgatcgcac taacatgaac acgcaacctt tctgccagcg
    67861 taagcagcgt tgccagctct ttgctttgcg cggcagtcac gttataagcg gggctgagtg
    67921 agtcttcgct gtaatcgaag cgcagggagt ccggatccat atccagctcg cgggccatcg
    67981 tccctgacag ccaggccgtt tgctcccgct caccaaggga catcgacggg cgcggaaatg
    68041 accgctgtaa tgtgcgactg gcgggaaacg ccaacataat gtgatgacgc tgcggcagtt
    68101 cgcgactcca cggtaacaac gttttagcca gctgctgcgc atcaacaatc cgcccatctt
    68161 tgataatgtc gtgcgccagc ggcaaccgcc accagcgttg caaaaagcat tcttttgcgc
    68221 cccgcacgat cgcaaccgct gccgcttctt gctgttgtaa atgcaaacca atttgccaga
    68281 tcttaaatgt cattgtgatg atctccttat cacccgtcac tctgacgggt atatcaatgc
    68341 gtctggcttg cctttatact accgcgcgtt tgtttataaa ctgcccaaat gaaactaaat
    68401 gggaaatttc cagtgaagtt cgtaaagtat tttttgatcc ttgcagtctg ttgcattctg
    68461 ctgggagcag gctcgattta tggcctatac cgctacatcg agccacaact gccggatgtg
    68521 gtgacattaa aagatgttcg cctgcaaatt ccgatgcaga tttacagcgc cgatggcgag
    68581 ctgattgctc aatacggtga gaaacgtcgt attccggtta cgttggatca aatcccaccg
    68641 gagatggtga aagcctttat cgcgacggaa gacagccgct tctacgagca tcacggcgtt
    68701 gacccggtgg ggatcttccg tgcggcaagt gtggcgctgt tctccggcca cgcgtcacaa
    68761 ggggcgagta ccattaccca gcagctggcg agaaatttct tcctcagtcc agaacgcacg
    68821 ctgatgcgta agattaagga agtcttcctc gcgattcgca ttgaacagtt gctgacgaaa
    68881 gacgaaatcc tcgagcttta tctgaacaag atttaccttg gttaccgcgc ctatggtgtc
    68941 ggtgctgcgg cacaagtcta tttcggtaaa acggtcgacc aactgacgct gaacgaaatg
    69001 gcggtgatag ccgggttgcc gaaagcgccc tccaccttta atccgctcta ctcaatggat
    69061 cgtgccgtcg cgcggcgtaa cgtcgtactg tcgcggatgc tggatgaagg gtatatcacc
    69121 cagcaacagt tcgatcagac acgcactgag gcgattaacg ctaattatca cgcgccggag
    69181 attgctttct ctgcgccgta cctgagcgaa atggtgcgcc aggagatgta taaccgttat
    69241 ggcgaaagtg cctatgaaga cggttatcgc atttacacca ccatcacccg caaagtgcag
    69301 caggccgcgc agcaggcggt acgtaataac gtgctggact acgacatgcg ccacggctat
    69361 cgcggcccgg caaatgtgct gtggaaagtg ggcgagtcgg cgtgggataa caacaagatt
    69421 accgatacgc tgaaggcgct gccgacctat ggtccgctgc tgcctgccgc agtcaccagc
    69481 gccaatcctc agggagcgac ggcgatgctg gcggacgggt cgaccgtcgc attgagtatg
    69541 gaaggcgttc gctgggcgcg tccttaccgt tcggatactc agcagggacc gacgccgcgt
    69601 aaagtgaccg atgttctgca aacgggtcag caaatctggg ttcgtcaggt tggcgatgca
    69661 tggtggctgg cacaagtgcc ggaagtgaac tcggcgctgg tgtcgatcaa tccgcaaaat
    69721 ggtgccgtta tggcgctggt cggtggcttt gatttcaatc agagcaagtt taaccgcgcc
    69781 acccaggcac tgcgtcaggt gggttccaac atcaaaccgt tcctctacac cgcggcgatg
    69841 gataaaggtc tgacgctggc aagtatgttg aacgatgtgc caatttctcg ctgggatgca
    69901 ggtgccggtt ctgactggca gccgaagaac tcaccaccgc agtatgctgg tccaattcgc
    69961 ttacgtcagg ggctgggtca gtcgaaaaac gtggtgatgg tacgtgcaat gcgggcgatg
    70021 ggcgtcgact acgctgcaga atatctgcaa cgcttcggct tcccggcgca aaacattgtc
    70081 cacaccgaat cgctggcgct gggttcagcg tccttcaccc caatgcaggt ggcgcgcggc
    70141 tacgcggtca tggcgaacgg cggcttccta gtggacccgt ggtttatcag caaaattgaa
    70201 aacgatcagg gcggcgtgat tttcgaagcg aaaccgaaag tagcctgccc ggaatgcgat
    70261 attccagtga tttacggtga tacgcagaaa tcgaacgtgc tggaaaataa cgatgttgaa
    70321 gatgtcgcta tctcccgcga gcagcagaat gtttctgtac caatgccgca gctggagcag
    70381 gcaaatcagg cgttagtggc gaagactggc gcgcaggagt acgcaccgca cgtcatcaac
    70441 actccgctgg cattcctgat taagagtgct ttgaacacca atatctttgg tgaaccaggc
    70501 tggcagggta ccggctggcg tgcaggtcgt gatttgcagc gtcgcgatat cggcgggaaa
    70561 accggtacca ctaacagttc gaaagatgcg tggttctcgg gttacggtcc gggcgttgtg
    70621 acctcggtct ggattggctt tgatgatcac cgtcgtaatc tcggtcatac aacggcttcc
    70681 ggagcgatta aagatcagat ctcaggttac gaaggcggtg ccaagagtgc ccagcctgca
    70741 tgggacgctt atatgaaagc cgttcttgaa ggtgtgccgg agcagccgct gacgccgcct
    70801 ccgggtattg tgacggtgaa tatcgatcgc agcaccgggc agttagctaa tggtggcaac
    70861 agccgcgaag agtatttcat cgaaggtacg cagccgacac aacaggcggt gcacgaggtg
    70921 ggaacgacca ttatcgataa tggtgaggca caggaattgt tctgattaaa aaggcgcttc
    70981 ggcgcctttt cagttggctg acaaagtgca tttgtttatg ccggatgcgg gtgaacgcgt
    71041 tatccggcta acaaaatcgt gaaaaatcaa tatattgcag gaaccccata agcctgataa
    71101 gcgttgtgca tcaggcaaac ttcacgcatt tacactcgcc cctgcccttt caaccattcg
    71161 cgcacgagga acagcgcgct gacattacgc gcttcgttga agtcagggtc ttccagcaaa
    71221 tccatcatat gcgccagcgg ccagcgcacc tgtggtagcg gctctggctc atcgccttcc
    71281 agcgattccg ggtagagatc ttgcgcgacc acgatattca ttttactgga aaagtaagac
    71341 ggtgccatgc tgagcttctt caaaaaagtc agatcgttcg caccaaatcc aacctcttct
    71401 tttagctcgc ggttagcggc ttcgtagacg ctttcacccg gatcaattaa tcctttcgaa
    71461 aaacctaatt cgtaggattc agttcccact gcgtattcgc ggatcaggat caggtgatcg
    71521 tccacaatcg gcacaatcat cactgcttcc cggttggttg gacgcattcg ttcataaaca
    71581 cgccgcacgc cattgctgaa ctccagatcc acactctcga cggtaaacag tcgggaacgg
    71641 gctacagttt caacattcag aatggtgggt ttttgtaatg atttgttcat cgtgggatct
    71701 atgctgtgaa atcagcggtt attgtgcgat atcggacacg ctttcggcaa tgtgaattgc
    71761 atgttattta catttatgta acttaataaa taatcgtcct caaatcaaat taaaagtcaa
    71821 taggttgaaa taactccagg aatttgctga tattccgcct tcagagggtt tgctatgatc
    71881 agcggttact gtgatgtgct taatgatgct caagttaaac tccacgcttg ccgatagcca
    71941 accgcagaat catgtattgt gtccggtgcg actgaccacg cctgacagac taagtaagat
    72001 ggggaaagca tgagcaccat tgtgattttt ttagctgctt tgctggcctg ctcactactt
    72061 gcgggatggc tgataaaagt gcgatccaga cagcgtcagc tgccctggac caacgccttc
    72121 gcggatgcgc aaacgcgtaa actcacacct gaagaacgta gcgccgttga aaattatctt
    72181 gagagcctga cgcaggtatt acaggtgcct ggcccaacgg gagctagcgc ggcaccgatc
    72241 tctctggtgc tgaatgccga aagcaacaac gtcatgatgc tgacacacgc tatcacacgt
    72301 tacggcatct ctaccgacga tccgaataaa tggcgttact acctcgattc ggtagaagtc
    72361 cacctgcccc ccttctggga acagtacatc aacgatgaga ataccgttga actgattcat
    72421 accgattcgc tgccgttggt tatttcactc aacggtcata cgctgcagga gtacatgcag
    72481 gaaacgcgcg gctatgcctt gcaacctgtt ccgtccacgc aggcgtcgat tcgcggggaa
    72541 gagagtgagc aaatcgagct actcaatatt cgcaaagaaa cgcatgaaga atatgcgctg
    72601 agtcgtccgc gcgggctgcg tgaagcgttg ctgatcgtcg cctccttcct gatgttcttt
    72661 ttctgcctga ttaccccgga tgtatttgtt ccgtggctgg caggcggcgc attactgctg
    72721 ctgggcgcag gtctgtgggg gctattcgcg cccccggcaa aatcctccct gcgagaaatt
    72781 cattgtctgc gcggtacacc ccgtcgttgg ggattgtttg gcgaaaacga tcaggaacag
    72841 atcaacaata tttcactcgg tattatcgac ctggtctatc ccgcacactg gcagccatac
    72901 attgctcagg atctcggtca acaaaccgat attgatatct atctcgaccg ccacgtagtg
    72961 cgtcaggggc gatatctttc gctgcatgat gaagtaaaaa actttccgtt acagcactgg
    73021 ctgcgcagta cgattatcgc tgcgggctcg ctgctggtgc tgtttatgct gttattctgg
    73081 atcccgctgg atatgccgct gaaattcact ctctcatgga tgaaaggcgc gcagaccatt
    73141 gaagccacca gcgtaaaaca actggctgac gctggcgtgc gggtaggcga tacattgcgt
    73201 attagcggta cgggaatgtg taatattcgc acttccggaa cctggagcgc gaaaaccaat
    73261 tcaccttttt taccatttga ctgctcgcag atcatctgga atgacgcccg ctcattacca
    73321 ttaccagaat ctgaactggt caacaaagcg acggcattga ctgaagcagt taatcgccag
    73381 ctgcacccta aaccggaaga tgaatctcgc gtcagtgcct cattacgttc agcgattcaa
    73441 aaatccggca tggtattgct tgatgatttt ggcgacattg tactgaagac agcggattta
    73501 tgttctgcca aagatgactg tgtgcgactg aaaaatgcgc tggtcaatct cggcaacagt
    73561 aaagactggg acgcgctggt aaaacgcgcc aacgctggga agctcgatgg cgtgaatgtg
    73621 ttattacgcc cggtgagtgc ggaatcgctg gataacctgg tggcaacctc caccgcgccg
    73681 tttatcacgc atgaaacggc gcgagcggca caatcactaa acagtccggc ccccggcgga
    73741 ttcctgattg tcagcgacga aggcagcgat tttgttgatc agccctggcc ttcggcatca
    73801 ctttacgact acccgccgca agaacagtgg aacgctttcc agaaactggc acaaatgctg
    73861 atgcatacgc cgtttaacgc cgaaggtatc gtcacaaaaa tcttcactga cgccaatggt
    73921 acgcagcata ttggccttca tccgatcccg gatcgttccg gcctgtggcg ctatctcagc
    73981 accacattgc tgctactgac gatgctgggt agcgccattt acaatggcgt acaggcctgg
    74041 cgtcgttacc agcgtcatcg cactcgcatg atggagattc aggcctatta tgaaagctgc
    74101 ctgaacccgc aactgatcac cccttcagaa agccttatcg aataacacgt ttgcgcggca
    74161 ggttatgcta ccctgtcgcg caaattgctt cactctggag atttccctca tgcatatcaa
    74221 cattgcctgg caggacgtag ataccgttct gctggatatg gacggcacgt tgctcgacct
    74281 cgccttcgat aactatttct ggcaaaagct ggtgcctgaa acatggggcg cgaaaaacgg
    74341 ggttacgcca caggaagcga tggaatatat gcgccagcaa tatcacgacg tacagcatac
    74401 gctaaactgg tactgtcttg attactggag tgagcaactg ggtctggata tctgtgcgat
    74461 gaccaccgag atgggaccgc gcgccgtact gcgtgaagat accattccgt ttcttgaggc
    74521 actgaaagcc agcggtaagc agcgaatttt gctcaccaat gctcatccgc acaacctggc
    74581 ggtaaaactt gagcatacgg gtctggacgc acaccttgat ttattacttt ccacccacac
    74641 atttggttat ccgaaagagg atcagcggtt atggcatgcg gtggccgaag ctacgggtct
    74701 gaaagctgaa agaacgctgt ttattgatga cagcgaagcg attctcgatg ctgccgcgca
    74761 atttggtatt cgttactgcc tcggcgtgac taatcctgat tccgggattg ccgagaaaca
    74821 gtatcaacgc catccgtcac tgaatgacta ccgccgcctg atcccctcgc taatgtgaag
    74881 gagacgccat gaaagagaaa cctgctgttg aggttcgact ggataaatgg ctatgggctg
    74941 cccgttttta taaaacccgc gcgctggccc gtgaaatgat taaaggcggt aaggtgcatt
    75001 acaacgggca gcgcagcaag ccgagcaaaa tcgtcgagct gaatgccacg ctcactctgc
    75061 gccagggaaa tgacgaacgc acggtgattg taaaggcgat tactgaacag cgtcgccccg
    75121 ccagcgaggc agccttgctg tatgaagaga ctgcggaaag tgtagagaaa cgcgaaaaaa
    75181 tggcgatggc acgtaaactt aatgccttaa ccatgccgca cccggaccga cgcccggaca
    75241 aaaaagagcg ccgcgacctg ttacgattta aacacggcga cagtgaataa ctgtcacctg
    75301 caagagagat gattatgccg caacatgacc aattacatcg ctatctgttt gaaaattttg
    75361 ccgttcgcgg cgaactggta accgtttcgg aaaccctgca acagatcctt gagaaccacg
    75421 attatccgca gcccgttaaa aacgtgctgg cagaactgct ggttgcgacc agcctgttaa
    75481 ccgctacgct gaagtttgat ggtgatatca ccgtacagct gcagggcgat ggtccgatga
    75541 atctggcggt tattaacggt aacaataacc agcaaatgcg cggtgtggcg cgcgtgcagg
    75601 gcgaaattcc agaaaatgcc gacctgaaaa cgctggtcgg caatggttac gtggtgatca
    75661 ccattacccc gagcgaaggc gaacgctatc agggcgtagt tggtctggaa ggtgataccc
    75721 tggcggcctg cctagaagat tactttatgc gttctgaaca gctgccgacg cgcctgttta
    75781 ttcgcaccgg cgacgtagac ggcaaaccgg ctgcaggcgg tatgttgttg caggtaatgc
    75841 ctgcgcaaaa tacccagcag gacgactttg accacctggc gacgctaacc gaaaccatca
    75901 aaaccgaaga actgctgacc ttaccggcaa acgaagtgtt gtggcgtttg tatcacgaag
    75961 aagaggtgac ggtttacgat ccgcaggatg tggagttcaa atgcacctgc tcgcgtgaac
    76021 gttgcgccga tgcgctgaaa acgctgcctg atgaagaagt tgatagcatc ctggcggaag
    76081 atggcgaaat tgacatgcat tgtgattact gcggtaacca ctatctgttc aatgcgatgg
    76141 atattgctga aatccgcaac aacgcgtctc cggcagatcc gcaagttcat taatgagtat
    76201 gtccggcaga gagggctctg ccggatttta cgacttaatg gcctgatgtg ctgcgcttac
    76261 cagcgcctat gaaataaatc gcaaattatt ttggtcagat acagcgtcgt atccgataaa
    76321 agcgttgtca cttacttaag tttatttcca gcgtttcacc atctaccgtc agatcgcgcc
    76381 acgctttcgg cctcgtcccc agttttccat ccttcgcagc tgtgagtaat ttttctttcg
    76441 ttatttcgtt cggaagtaaa ctcatatcaa gcgcgtccca ttcttttctg tctgagtcaa
    76501 agccatagac aatgactctg tcatcattaa aagcaaacag gatccgctcc gcctgaccat
    76561 cactattcag atcttgctcg accagtacgc aggcgtcttt ttcaatacag gtcatcacgt
    76621 tatagcgatc ctggattaag gccgaccaga atgtcgcatc cggtttaaca gaaccagggg
    76681 caattaacac attttcggct aatacttttt ccgatacctg ttcctgaaga tgttgctctc
    76741 catctaacgc catcagcaga tcccgcgcgc gtttcgggtc tttcataaac ccggcatcgc
    76801 ttttcagcga ctcaagcgcc gcacgtccat agcgaccgct ctgttcgagc atgtagatag
    76861 ttacctggtc tggcgtgttt ttgccgctct gataacgcgc catatgactg ttcacgctaa
    76921 tacgcatact gtccagcacc ggcgaattaa gaagcaccag tatcaccaac accaataacg
    76981 aaaccgcaag gttcactttg ccctgaagaa caaggggatt ttgcccatta cgccagacga
    77041 tactgacaaa ataccccagc gaccacacca gtaacaccag ctccgccagc gcgccctgca
    77101 agcggtcgac agtccagccg tactgagcga cccgcagcca taacccccag gtggcgacga
    77161 acacatacag cggagcaacc agcaaagcgg ttttaatcag gcaacgcaac ggccccgtcc
    77221 agggaagtga cgctttttgc ggatcgcgga caatagccat taagatcaat tgcaaaaagg
    77281 ccagcgttaa cagcaacccg gcggcggaga tgtggcgaga aatcgcgctc aggcccgtaa
    77341 acggcagggt gatgataaac atcagggtta gcaatgatac taacggcagc aaccccgtgg
    77401 cgattagcgt gaacaacttt tgaatagagt cgattaaacg tgactgtgta cgcgcgagga
    77461 tcaccgccag tgcggtaacc agaccaaatg taagatacat aaaccagtcg gttgcaaaaa
    77521 agagcgtatt gaaaaacgtg atgccaacaa gtttaaacaa ctcaccccac agtaaaagca
    77581 ccagccacgt caggccattg gagagaaaaa tcaccagtaa tataagtacg ttatgccata
    77641 ctgactggta aaagtagcta tagcggctac tgccattgcg gatacgaagg ctttgttgta
    77701 tccacggtag caacaacatt gccatcaaca gcagatagca gccaaaatcc caaatcgcct
    77761 tttcagctct ccaggggttc atgccatcag tttgccactt cagccaaccg ctcatccctg
    77821 tcgtggcaat aaacaccagc gccagccatc cccaaaggcg cttctgttta aaagagatca
    77881 cggagaagag taaaacggat gaaaacgcga cggtcgcagg cacgccataa acaatccaac
    77941 tgtgattttt tccggcaagc caggcgataa gcaggtagca aaccagcccc tgaagtaatc
    78001 cggtagcaat catcccccag cgtgttgccg gtgaaagttc aacgttatcc atgttggtta
    78061 tccagaatca aaaggtgggt taattatcgc atccgggcag tagtattttg cttttttcag
    78121 aaaataatca aaaaaagtta gcgtggtgaa tcgatacttt accggttgaa tttgcatcaa
    78181 tttcattcag gaatgcgatt ccactcacaa tattcccgcc atataaacca agatttaacc
    78241 ttttgagaac attttccaca cctaaaatgc tatttctgcg ataatagcaa ccgtttcgtg
    78301 acaggaatca cggagttttt tgtcaaatat gaatttctcc agatacgtaa atctatgagc
    78361 cttgtcgcgg ttaacacccc caaaaagact ttactattca ggcaatacat attggctaag
    78421 gagcagtgaa atgcgcgtta acaatggttt gaccccgcaa gaactcgagg cttatggtat
    78481 cagtgacgta catgatatcg tttacaaccc aagctacgac ctgctgtatc aggaagagct
    78541 cgatccgagc ctgacaggtt atgagcgcgg ggtgttaact aatctgggtg ccgttgccgt
    78601 cgataccggg atcttcaccg gtcgttcacc aaaagataag tatatcgtcc gtgacgatac
    78661 cactcgcgat actttctggt gggcagacaa aggcaaaggt aagaacgaca acaaacctct
    78721 ctctccggaa acctggcagc atctgaaagg cctggtgacc aggcagcttt ccggcaaacg
    78781 tctgttcgtt gtcgacgctt tctgtggtgc gaacccggat actcgtcttt ccgtccgttt
    78841 catcaccgaa gtggcctggc aggcgcattt tgtcaaaaac atgtttattc gcccgagcga
    78901 tgaagagctg gcaggtttca aaccagactt tatcgttatg aacggcgcga agtgcactaa
    78961 cccgcagtgg aaagaacagg gtctgaactc cgaaaacttc gtggcgttta acctgaccga
    79021 gcgcatgcag ctgattggcg gcacctggta cggcggcgaa atgaagaaag ggatgttctc
    79081 gatgatgaac tacctgctgc cgctgaaagg tatcgcttct atgcactgct ccgccaacgt
    79141 tggcgagaaa ggcgatgttg cggtgttctt cggcctttcc ggcaccggta aaaccaccct
    79201 ttccaccgac ccgaaacgcc gcctgattgg cgatgacgaa cacggctggg acgatgacgg
    79261 cgtgtttaat ttcgaaggcg gctgctacgc aaaaactatc aagctgtcga aagaagcgga
    79321 acctgaaatc tacaacgcta tccgtcgtga tgcgctgctg gaaaacgtca ccgtgcgtga
    79381 agatggcact atcgactttg atgatggttc aaaaaccgag aacacccgcg tttcttatcc
    79441 aatctatcac atcgataaca tcgttaagcc ggtttccaaa gcgggccacg cgactaaggt
    79501 tatcttcctg actgctgatg ctttcggcgt gttgccgccg gtttctcgcc tgactgccga
    79561 tcaaacccag tatcacttcc tctctggctt caccgccaaa ctggccggta ctgagcgtgg
    79621 catcaccgaa ccgacgccaa ccttctccgc ttgcttcggc gcggcgttcc tgtcgctgca
    79681 cccgactcag tacgcagaag tgctggtgaa acgtatgcag gcggcgggcg cgcaggctta
    79741 tctggttaac actggctgga acggcactgg caaacgtatc tcgattaaag atacccgcgc
    79801 cattatcgac gccatcctca acggttcact ggataatgca gaaaccttca ctctgccgat
    79861 gtttaacctg gcgatcccaa ccgaactgcc gggcgtagac acgaagattc tcgatccgcg
    79921 taacacctac gcttctccgg aacagtggca ggaaaaagcc gaaaccctgg cgaaactgtt
    79981 tatcgacaac ttcgataaat acaccgacac ccctgcgggt gccgcgctgg tagcggctgg
    80041 tccgaaactg taatgatttg aagctggaga atatctatcc agtatcttat agaaagcaaa
    80101 acgggaggca ccttcgcctc ccgtttattt acccttcttt tgtcgtgccc tgcgcccgcg
    80161 ttaccggcac tggcagccag gcgcgaatgg aaagcccgcc ccgctcgctg gtgccaagct
    80221 ccagcatccc gttatggtta tccacgatac gctgcacaat tgccagccct aatcccgtgc
    80281 cgctaatggt gcgcgcactg tcgccgcgga caaacggctg gaacaggtgc ttacgttgtt
    80341 ccggcgcaat tcccggaccg tcatcttcca cctggaacca ggcgcgattc ggctccgttc
    80401 cgctgctgac tttgatccag ccattgccat aacgggcggc gttgaccacc atattcgcca
    80461 ccgcgcgttt gatcgacagc gggtgcattt tcacttcaat gctgccgggg taaagcgcgg
    80521 tttcaatttc ccgctcatag ccactttcgg cagcaatcac ctcaccgagc actgcattaa
    80581 gatccgccat ttccatcggc atctcctgcc cggtgcgcag gtagtcgata aactgctcaa
    80641 tgatggcgtt gcactcttcg atatctttat tgatcgattc tgccagatag ccatcctgct
    80701 cgctcatcat ctcagtcgcc aggcgaatac gcgtcagcgg cgtgcgcaag tcgtgactta
    80761 cccccgccat cagcagcgtg cggtcatccg ccagttgctt aacaccagcc gccatatggt
    80821 taaaggcacg ggtaacggaa cgcacctccg aagcgccata ttcacgcagc ggcggcggaa
    80881 taatcccttt accaacctgc aaggctgcgt gttcgagatc gaccaacggt cggttctgga
    80941 tacgaataaa cagccacgcc ccgcctatcg ccaatagcat aatcgccagc gtatagcgga
    81001 acagcggaga gaaatcgccc tgatgaattt cggtcagcgg cacgcgtacc cagatattgg
    81061 gcgacagcca ggttttcagc cagacgacag gcgaactttt gttgacctca acgcgcactt
    81121 ccgtcgggcc gcccagttgc tgtgccatct gatggcttaa gaattcatag tgttgcgccc
    81181 aacgcagacc tgcctcttcg gcagcctcgt tggagtagag agagatcccc agctcacggt
    81241 agatctcccg acggaaagcg ggaggcacaa ccaactgcgt gccgtcctcc agttgcagtt
    81301 tatcggtcat caacatacgc acttcgtacg cgaggacttt attaaactgc tggaggctcg
    81361 gcaaaatcgc gaagttcagc accaccagat aagtcgtcac caggctggcg aacagcaagg
    81421 tgacgatgag caataacgta cgggcaaatg aacttcgtgg cgagaagcgc aatcgcctca
    81481 tgctttagag ccgtccggta caaagacgta gcccaggccc caaacggtct gaatgtaacg
    81541 cggatgcgct ggatcttctt ctaccatgcg gcgcagacgc gaaatctgca cgtcgatgga
    81601 gcgttccatt gcggaatatt cacgaccacg ggcaaggttc atcagcttat cgcgggagag
    81661 cggctcacgc ggatggctga ccagtgcctt cagtaccgca aactcaccgc tggtgagcgg
    81721 catcggctca tcttcgcgga acatttcgcg cgtaccgagg ttaagtttga acttaccgaa
    81781 agcaattacc gcctcttcct gtgacggtgc gcctggcagt tcgttcgcct gacgacgcag
    81841 caccgcacga atacgggcca gcagttcacg cgggttaaac ggttttggaa tgtagtcgtc
    81901 agcgccaatc tccaggccta cgatacggtc cacttcttcc cctttcgccg tcaccataat
    81961 gatcggcatc gggttgctct gactacgaag acgtcggcaa atcgacaagc catcttcacc
    82021 aggtaacatt aaatccagta ccataagatg gaaagattca cgagtcagca ggcgatccat
    82081 ctgttctgca ttagcgacgc ttcgaacctg gaagccttgt tcggtgagat aacgttccag
    82141 cagcgcacgc aggcgcatgt cgtcatcgac caccagaatc ttgtagttct cttgcattgt
    82201 ttgtactccc aaaggttcgc aacaatttgt aagcgtgtat tcttaaaaaa gctcacgttc
    82261 gtcaccagct aaatctggta tgaatttcag cctaaattgt tacaaagcat attaaacagc
    82321 agcttaagta tacaatttat tcggcgaaac attattgatt ctgttgatat gatcacgtta
    82381 tacccaatgt gcgcattatc aaacagacaa agggaatcaa cgagatgaaa acgcccctgg
    82441 ttacccggga agggtatgaa aaactcaaac aagagcttaa ttatctctgg cgtgaagaac
    82501 gcccggaggt cacaaaaaag gtgacctggg ccgcaagtct gggcgaccgc agcgaaaatg
    82561 ctgactatca gtataataaa aagcgtctgc gtgaaatcga ccgtcgcgtg cgctatctca
    82621 ctaaatgcct ggaaaatctc aaaattgtcg attactcccc tcagcaggaa ggcaaagtct
    82681 tttttggcgc gtgggtggag attgaaaacg acgatggcgt gactcaccgt ttccgtattg
    82741 tcggctacga tgaaattttt ggccgtaaag attacatctc tatcgattcc ccgatggccc
    82801 gcgcattgct gaaaaaagaa gtcggcgatc tggcggtggt gaatacccct gccggggaag
    82861 cgaactggta tgttaatgct atcgagtacg tgaaaccgta aggaagagtc ttaacctcct
    82921 gccgatggct ggcatttttg ccagccagtc cgtataacta tcccctgatt tttgatccga
    82981 aaagatgaac tcaaaccatg atgaatgatt cgttctgccg cattattgcg ggtgaaattc
    83041 aggcgcgccc ggaacaggtt gacgctgccg ttcgcctgct tgatgaaggg aataccgtgc
    83101 cgtttatcgc acgttatcgt aaggaaatca ccggcggtct ggatgacacg cagctgcgta
    83161 atctggaaac gcgtctgagc tatctgcgcg agctggaaga gagacgtcag gcgatcctca
    83221 aatccatttc cgagcaaggc aaactcaccg atgatctggc gaaggccatc aacgccaccc
    83281 taagcaaaac cgaactcgaa gacctctacc tgccctacaa acctaaacgc cgcacccgcg
    83341 ggcaaatcgc cattgaagca gggcttgagc cgttggctga cctgctgtgg agcgatccgt
    83401 cacacacgcc agaagtcgcc gctgcacaat atgttgatgc cgataaaggc gtggcagata
    83461 ccaaagccgc gctggatggc gcgcgctata tcctgatgga acggtttgcc gaagatgccg
    83521 cgctgctggc gaaagtgcgt gattatctgt ggaagaacgc gcatttggtt tctacggtgg
    83581 tgagcggtaa agaagaggaa ggggcgaaat tccgcgacta tttcgatcat cacgaaccgt
    83641 tgtccacggt gccttctcac cgcgcgctgg cgatgttccg tgggcgtaac gaaggcgtac
    83701 tccagctttc gctgaatgcc gatccgcagt tcgatgagcc gcccaaagag agctattgcg
    83761 agcaaatcat catggatcac cttggcctgc gcctgaacaa tgccccggcg gatagctggc
    83821 gcaaaagcgt agtgagctgg acctggcgca tcaaggtgct gatgcatctg gaaaccgaac
    83881 tgatgggtac cgtgcgcgaa cgcgcggaag atgaagcaat caacgtcttt gcccgtaacc
    83941 tgcacgatct gctgatggcg gcccctgccg gactgcgtgc aacgatgggc ctcgatccgg
    84001 gtctgcgtac cggggtaaaa gtggcggtgg tcgatgccac tggcaaactg gtggcgaccg
    84061 ataccattta cccgcacacc ggacaagccg caaaagcagc gatgaccgtt gctgccttgt
    84121 gtgaaaaaca taacgttgaa ctggtggcga tcggcaacgg tacagcttcc cgcgaaactg
    84181 aacgtttcta tctcgacgtg cagaagcagt acccgaaagt gaccgcacag aaagtgatcg
    84241 tcagcgaagc tggcgcgtcg gtttactcag cttccgagct ggcagcgcag gagttcccgg
    84301 atctcgacgt ttcgctgcgt ggtgcggtat ctatcgcccg ccgtttgcag gatccgctgg
    84361 cggagctggt gaaaatcgat ccgaaatcta tcggcgtagg tcagtatcag catgacgtca
    84421 gccagacgca actggcccgc aaactggatg cagtagtaga agactgcgta aacggcgttg
    84481 gcgtcgatct caacaccgct tctgttccgc tattaacccg cgtggcgggc ctgacgcgca
    84541 tgatggcgca aaacatcgtg gcctggcgcg atgagaacgg ccagttccag aaccgtcagc
    84601 aactgctgaa agtcagccgt ctggggccga aagccttcga gcagtgcgcg ggcttcctgc
    84661 gcattaacca cggtgataac ccgctggacg cctctaccgt tcatccggaa gcctatccgg
    84721 tggtggaacg cattctggca gcaacacagc aggcactgaa agatctgatg ggtaacagca
    84781 gcgaactgcg taacctgaaa gcgtctgact ttactgatga aaaattcggt gtgccgacag
    84841 taactgacat catcaaagag ctggaaaaac cgggtcgcga tccgcgtccg gaatttaaaa
    84901 ccgctcagtt tgccgatggc gtcgagacaa tgaacgacct gcaaccgggt atgatcctcg
    84961 aaggcgcagt gaccaacgtc accaactttg gcgcgtttgt cgatattggc gtgcatcagg
    85021 acggcctggt tcacatctct tcattgtcga acaagtttgt ggaagatccg cataccgtgg
    85081 tgaaagcggg cgacattgtg aaggtgaaag tgctggaagt ggatcttcag cgtaaacgta
    85141 tcgccctgac tatgcgtctg gacgagcagc ctggcgaaac caactctcgt cgcggcggcg
    85201 gtaatgaacg cccgcaaaac aaccgcccgg cagccaaacc acgcggtcgt gaagcgcagc
    85261 ctgccggtaa tagcgcgatg atggatgcgc tggcggcagc gatgggtaaa aaacgttaaa
    85321 cgcccggact ggcccctaaa cctgcgcgat aaggcgttca cgccgcatct ggcaacgaac
    85381 gctttgcctg acataaaagt gccggagaat atctccggca cttttattcc acagccaaac
    85441 tcacaatata ctccgttaat atttatcact tcattaacaa ctgaaacctt aattaaacat
    85501 tagccagtcc gggtaactca ctattcgaat tatattttcg ctgcgatata accttgagcc
    85561 acatcaacat tgagtcagat tattattcaa accaacattc gtacacattt taagtattgc
    85621 tgatagaaac cattctcatt atcattgtgt tgttgattat ttaatctctc ctttgttggc
    85681 aaatcatctg gtctcatgtc gcggtcaaac gccccatgag gtagttatcc agttaatgag
    85741 aaacaagtag gcacctatgc aatacactcc agatactgcg tggaaaatca ctggcttttc
    85801 ccgtgaaatc agcccggcat atcgccaaaa actgctttct ctaggcatgt tacctggctc
    85861 ctcttttgat gtggtgcgcg tcgctccact cggcgacccc attcatatcg aaacccgtcg
    85921 tgtgagcctg gtattacgca aaaaagatct ggccttatta gaagtggaag cggtttcctg
    85981 ttaatacggt gataacaaca atgaaaaaat taaccattgg cttaattggt aatccaaatt
    86041 ctggcaagac aaccttattt aaccagctca ctggcgcacg tcagcgtgta ggtaactggg
    86101 ctggcgttac cgtcgaacgt aaagaagggc aattctccac caccgatcat caggtcacgc
    86161 tggtggacct gcccggcacc tattctctga ccaccatctc atcgcagacc tcgctcgatg
    86221 agcaaatcgc ctgtcactac attttgagtg gcgacgccga cctgctgatt aacgtggtgg
    86281 atgcgtctaa ccttgagcgt aacctgtacc tgacgctgca actgctggaa ctcggcattc
    86341 cctgcatcgt ggcgctgaac atgctcgaca ttgccgagaa gcaaaatatt cgtattgaaa
    86401 ttgatgctct atcggcacgt cttggctgcc cggtgatccc gctggtttca acccgtggtc
    86461 gcggtattga agcgctcaag ctggcgattg atcgctataa agctaacgag aatgtggaac
    86521 tggtgcatta cgcacagccg ctactcaacg aagcagattc actggcaaaa gtgatgcctt
    86581 ccgacatccc gctgaaacaa cgtcgctggc tgggcctgca aatgctggaa ggcgatatct
    86641 acagccgcgc ctacgccggt gaagcgtcgc agcatctgga tgccaccctc gcccgtctgc
    86701 gtaatgaaat ggatgatccg gcgctgcaca ttgccgatgc gcgttaccag tgcattgctg
    86761 ccatctgtga tgtggtaagc aacaccctga cggcagaacc cagccgtttc accactgcgg
    86821 tagataaaat cgtgctcaac cgtttcctcg gtctgccgat tttcctcttt gtgatgtacc
    86881 tgatgttcct gctggctatc aacatcggcg gggcgttaca gccgctgttt gacgtcggct
    86941 ccgtggcgct atttgtgcat ggtattcaat ggattggcta cacgctccac ttcccggact
    87001 ggctgactat cttcctcgcc cagggcctgg gtggcggtat taacaccgtg ctgccactgg
    87061 tgccgcagat cggcatgatg tacctgttcc tctccttcct tgaggactcc ggctatatgg
    87121 cgcgtgcagc gtttgtgatg gaccgcctga tgcaggcgct gggcttgccg gggaaatcct
    87181 ttgtgccgct gatcgtcggt ttcggttgta acgtaccgtc ggtaatgggc gcacgtacgc
    87241 ttgatgcacc gcgtgaacgt ctgatgacca tcatgatggc accgtttatg tcctgcggcg
    87301 cgcgtctggc tatcttcgca gtattcgcgg ctgccttctt cgggcagaac ggtgccctgg
    87361 cggttttctc gctgtatatg ctcggtatcg tgatggcggt gctgactggc ctgatgctca
    87421 agtacaccat catgcgcggt gaagcaacgc cgtttgtcat ggagctgccg gtctaccatg
    87481 taccacacgt taaaagcctg attatccaga cctggcagcg tctgaagggc ttcgttctgc
    87541 gtgctggtaa agtgattatc atcgtcagca ttttcctgag cgctttcaac agcttctcgt
    87601 tgagcgggaa aatcgtcgat aacatcaacg actcggcgct ggcttccgtc agccgggtga
    87661 taacccctgt cttcaagccg attggcgtac atgaagataa ctggcaggca acggttggcc
    87721 tgtttacggg cgcaatggca aaagaagtgg tggtgggtac gctcaacacc ctctacaccg
    87781 cagaaaatat tcaggacgaa gagttcaatc cggcggaatt caacctcggt gaagagctgt
    87841 tcagtgcggt agatgaaacc tggcagagcc tgaaagacac cttcagcctt agcgtactga
    87901 tgaaccccat tgaagctagc aaaggcgacg gtgaaatggg taccggggcg atgggcgtga
    87961 tggatcagaa attcggtagc gcagctgccg catacagcta cctgattttc gtcctgctgt
    88021 atgtaccatg tatctcggtg atgggagcca tcgcccgtga atcaagccgt ggctggatgg
    88081 gcttctccat cctgtggggg ctgaatatcg cttactcact ggcaacattg ttctatcagg
    88141 tcgccagcta cagtcagcac ccaacttaca gcctggtgtg cattctggcg gtaatcctgt
    88201 ttaacatcgt agttatcggt ctgctgcgcc gcgcgcgtag ccgggtggat atcgaactgc
    88261 tggcaacccg caagtcggta agcagttgct gcgcagccag caccaccggt gattgccatt
    88321 aatggcttca cttattcagg tacgtgattt gctggcgtta cggtgccgta tggaagcgac
    88381 ccagataagc cagacattga acactccgca gccaatgatt aacgccatgc tgcaacagct
    88441 ggaaagcatg ggcaaagccg tgcggattca ggaagaacct gacggctgcc tctctggcag
    88501 ttgtaaaagc tgcccggaag gcaaagcctg tctgcgcgag tggtgggcgc tgcgttaacc
    88561 atactccatc gcctgttttg aaaagcccgg tatgcgtttg catccgggct tttttgcgtg
    88621 cggctttcca taaaaatgca attcttgcag cgcaacgtaa gttcctctga aagcgtctcg
    88681 cagggatgaa aactcgctaa tgcacagatg tggagtggcg cgtagagtcg cggcattcaa
    88741 acaacaggtg aaggaacgcc atgagtaaaa agcagagttc caccccacat gatgcgctgt
    88801 tcaaactctt tttacgccaa ccggagacgg cacgcgattt tcttgcgttt catttaccgg
    88861 cacccattca cgcgctctgt gatatgaaaa ccctcaagct ggagtcgagc agctttattg
    88921 atgacgatct gcgtgaaagc tattccgatg tgctgtggtc ggtgaaaacg gaacaaggac
    88981 caggatacat ctattgtctg attgaacatc aaagcacctc aaacaaactg atcgcatttc
    89041 gcatgatgcg ttacgctatt gccgcaatgc aaaatcacct tgatgctgga tacaaaacgt
    89101 tgccgatggt ggtgccattg ttgttttacc acggtattga aagcccctat ccctattcgc
    89161 tgtgttggct ggattgtttc gccgatccca aactggcaag gcagctttat gcctccgcat
    89221 ttccgctgat tgatgtcacc gtcatgcctg atgatgaaat catgcagcac cgacgtatgg
    89281 cgctgctgga gttaattcaa aaacatattc gtcaacgcga tctgatgggg ctggtagagc
    89341 aaatggcctg cttattaagt agtggatacg ctaatgacag acaaatcaaa gggctgttta
    89401 attacatact gcaaactggc gacgctgtac gttttaacga ttttatcgac ggcgttgccg
    89461 aacgttcacc gaaacacaag gagagtttaa tgactattgc ggaaagattg cggcaggagg
    89521 gggaacaatc caaagccctg catatagcca aaataatgct tgaatccgga gtccctcttg
    89581 cagacatcat gcgatttacc gggctgtcag aagaagagtt ggctgcggcg agtcagtaga
    89641 tactctaccc tgcttaagtg ctactttgtg ctatcttgca tgtaacacaa agtagcactt
    89701 agggaactga tatgcagaca gtgaaattac ggcaacaagg aggagccatg atcgttacta
    89761 tcccacgaga tcttgccatc gatcttggct ggtcaccggg cacagagctt accgtggaga
    89821 aaaaaggcga tagcgttaat ttgcgcgcca cagagcacaa accgcgtggg cgtcttaccg
    89881 tcgcgcagtt gttgagccaa atcgatgaaa gcgaaattac ggagctaaat cagtccacag
    89941 aaggatgggc tgaagggaaa aaaggtaatg aagcatggta aaggcacgga cgccacatcg
    90001 tggtgagatc tggtatttta accctgatcc ggttgccggg catgaacttc aggggccaca
    90061 ttattgcatt gtggtaacgg acaaaaaact caacaatgtt ttaaaagttg ctatgtgctg
    90121 cccgatttca acaggggcaa atgcagcacg ttccacaggg gtgacggtga acgtcctccc
    90181 ctgtgatacg caaaccggta acctgcatgg cgttgtactt tgtcaccagc taaaagccgt
    90241 cgatcttatt gcccgtggcg ctaaatttca taccgttgcc gatgaaaaat tgattagtga
    90301 agttatcagt aaactggtga atttaatcga cccacaataa ttagctctca cttaccacca
    90361 acaccctctg cttcaacgcc accagcaggt gacaaaactc gaccggatgc gaaataaacg
    90421 gcgcatgggc cgctttggcg aagatatatg attcgctgtg aggccaaagt ttatccagca
    90481 tcggcaccac tttgcgcggc accagaccgt cgagatagcc atacaatcgc aaaaacggca
    90541 tggacacgtt ttgcagcggc tgacggagat cgaccgtttt caggatttcc agcccgccat
    90601 taagcacgtc aacctccggc atcggtaacg ccagaacggt tttcttcaac gcccgcgcat
    90661 cctggcgcgc cgtttcagtc cccatggttt gtaacgccag gaaccgctcc actgtacgct
    90721 gaaaatcatc actgagttgc tgctgaaatc ccgccagcac gtccggtttt atccccggcc
    90781 actcgtcacg agcactaaaa taaggtgacg acgccacggt gaccagcgcc tgaacacgct
    90841 cgggatgggt taacgcaatc tggcttgcca ccagcccgcc cagactccag cctaaccaaa
    90901 tggctttatc aggtgcctgt tgcagcacgg cttcggccat atcagcaagt gacagcgcac
    90961 caaatccccg gctacgcccg aagccgggca ggtcaacaag gtgcagcgta aaatgcgagc
    91021 taagttcctc gtcaatgcaa cgccacactt cggcattcag tccccatccg tgcagcagca
    91081 caagatgaac attcccctga cctttggtct gccaccagat gttattcatc cgctattgtt
    91141 ctcttttgac ttacaaggat gaacatatgc taacagtacc gggattatgc tggctatgcc
    91201 gaatgccact ggcgttaggt cattggggga tttgttcggt ctgttcacgc gccacccgca
    91261 cagataaaac gttatgccca caatgtggat tacccgccac acactcccat cttccctgcg
    91321 gtcgctgcct gcaaaaaccg ccgccctggc aaagactggt cacggttgcc gactatgcgc
    91381 cgccgttaag tccgcttatc caccagctta aattttcccg gcgcagtgaa atcgccagcg
    91441 ccctgtcacg tctgttacta ttggaagtct tacacgctcg tcgcaccacc gggttgcaat
    91501 tgccggatcg catcatcagc gttccgttat ggcagcggcg tcactggcgt cggggattta
    91561 atcagagcga tttgctgtgt cagccgttat cacgctggtt gcactgccaa tgggatagcg
    91621 aagccgtcac acgtacacgg gccactgcga cccagcattt tctcagtgcc cggctgcgca
    91681 agcgcaacct gaaaaatgcc tttcgtcttg aattgcccgt gcaaggtcgc catatggtga
    91741 ttgtggatga tgtcgttacc accggaagta ccgtcgcaga gattgcgcag ttgcttttac
    91801 gcaatggtgc ggcgactgtc caggtctggt gcctttgtcg aaccttgtag agcctcgatg
    91861 atgggcgtat tataaccaac taaaatagtc aactattagg ccattactat gatccgtatt
    91921 tccgatgctg cacaagcgca ctttgccaaa ctgctggcaa atcaggaaga agggacacaa
    91981 atccgcgtat ttgtgattaa ccctggcacg cctaacgctg aatgtggtgt ttcttattgt
    92041 ccgccggatg ccgtggaagc caccgacact gccctgaaat ttgacctgct gaccgcgtat
    92101 gttgatgagt taagcgcacc atacctggaa gatgcagaga tcgactttgt taccgaccag
    92161 ttgggttctc agttaacgct gaaagccccg aacgccaaaa tgcgtaaagt ggcagacgat
    92221 gcaccgctga tggagcgcgt ggagtatatg ctgcagtcgc agatcaaccc acagcttgct
    92281 ggtcacggtg gtcgcgtttc gctgatggaa atcaccgaag acggttacgc cattctgcaa
    92341 tttggcggcg gctgtaacgg ttgttccatg gtcgatgtga cgctgaaaga agggatcgag
    92401 aagcagctgc tgaacgaatt cccggagctg aaaggtgtgc gcgatctcac cgaacaccag
    92461 cgcggcgaac actcctacta ctaagttatc ccctcttttg tagattgccc gatgcgacgc
    92521 taaagcgtca tttcgggcct acgttgaaca tgtgccagac gttggcacag cattgctttg
    92581 gcaaatccct cccttcgttt ccccgcgata atatgaccaa cctctcataa tttaaattta
    92641 ccccgctctg gtgattctca aacgccagat gttacccgta tcattcacat gggtaccaaa
    92701 catactcctg acatctgact acaataatta gttttagtgg gtatcagtcg tggtgccgca
    92761 atatctctgt tcccgattgg gataattaga gtttgtcgtc agaaaattga cgttacccat
    92821 aacaaatgaa aggccaggta aatcatgcca ttagtcattg ttgctatcgg tgtaatcttg
    92881 ttgttgctcc tgatgatccg cttcaaaatg aacggcttca tcgctctcgt cctcgtggcg
    92941 cttgctgttg gattaatgca aggaatgccg ctggataaag ttattggctc catcaaagcc
    93001 ggtgtcggcg ggacgctcgg tagccttgcc ctgatcatgg gttttggcgc aatgctggca
    93061 aactgcggtg gcgcacaacg tatcgccacc acgctgattg ccaaatttgg taaaaagcac
    93121 atccagtggg cggtggtgtt aaccggtttt accgttggtt ttgccctgtt ctatgaagtg
    93181 ggcttcgtgc tgatgctgcc gctggtgttt accatcgcgg cttccgcgaa tattccattg
    93241 ctgtatgttg gtgtaccaat ggcggctgca ctgtctgtga cccacggctt cctgccaccg
    93301 catccgggcc cgactgcgat tgccaccatt ttcaatgccg atatgggtaa aaccctgctg
    93361 tacggtacta ttctggcaat cccgaccgtg attctcgccg gtccggttta cgctcgcgtg
    93421 ttgaaaggta tcgataagcc aatcccggaa ggtctctaca gcgcgaaaac cttcagcgaa
    93481 gaagagatgc cgagctttgg cgtcagcgtc tggacctctc tggtgccggt tgtgctgatg
    93541 gcgatgcgtg cgattgccga aatgatcctg ccgaaaggtc acgctttcct gccggtagcg
    93601 gagttcctcg gtgacccggt aatggcaacg ctgattgccg tgctgattgc gatgttcacc
    93661 tttggtctga accgtggtcg ttcaatggat cagattaacg acacgctggt ttcttccatc
    93721 aaaatcattg cgatgatgct gttgatcatc ggtggtggcg gtgcgttcaa gcaggtgctg
    93781 gtagacagcg gcgtggacaa atacattgct tccatgatgc acgaaaccaa catttctccg
    93841 ctgctgatgg cctggtcgat tgctgccgta ctgcgtatcg cgctgggttc cgcaaccgtt
    93901 gcggcaatca ctgcgggtgg tatcgcggca ccgctgattg caacgacggg tgttagccca
    93961 gagctgatgg ttattgcggt gggttccggt agtgtgattt tctctcatgt gaacgatccg
    94021 ggcttctggc tgttcaaaga gtactttaac ctgactatcg gcgagaccat caaatcctgg
    94081 tcgatgctgg aaaccattat ctcggtgtgc ggtctggtag gctgtctgct gctgaatatg
    94141 gtgatttgat aacactgact gccggatgca tcgcatccga catcaaccgc actctacttc
    94201 ttcttcgctg cagctctgcg ccgtctgtcc aaatccttca gcaacttgtt cacgccatca
    94261 tcggcaaaca tcgactcaag cgttgcggaa agcttgcgtc gccagttttt atactggtaa
    94321 ctggtgccag gaatattcac cggttcggcc atatccagcc agtcttccgg ctgtagtcct
    94381 aacagagcac tgttactgtc ggcaatgtag cgctgcaaac cacggttcag cgtcggcgtc
    94441 atcgacatca acgatgcctt atgcccggca cgttttggca gacaaccata tttatgcagt
    94501 gcatccagca gcccttgctt cgccagttcg cgatcctgat acagaccgcg cagtaccact
    94561 tcatccggat acagccccag ggttttgccc agcgttagat ccccgctctc ccagtaacca
    94621 cgcagcgttg gcaggtcatg tgtcgccgca accgccatcg actgctccgg atacgctttc
    94681 ggtgcacgga acgtcttctc gtggtcgttt tcgaaataga gcactttgta agagtacaca
    94741 ccgctgctgc gcagcttacc gacaatctct accggtacgg taccgagatc ttcaccaatc
    94801 accatacagc gatgacgttt actttcgagt gccagaatcg agagcagatc atccaccgga
    94861 tagtgaacat acgcgccctg atctgccgtc tcgccatacg gtatccacca caaacgcagc
    94921 atcgacatca catggtcaat tcgtaatgcg ccgcagtttt gcatattggc acgcaacagc
    94981 tcgataaacg gttcataggc acgcgcggtg atgatatgcg ggtccattgg cggtaatccc
    95041 cagttctgcc ccaacgggcc gaggatatcc ggcggcgcgc caaccgatgc tttcaggcaa
    95101 tatagttcac ggtcacacca ggtttccgcc ccaccttccg ctacgccaac cgccagatca
    95161 cgatacaagc caatcggcat ttcatagccc tggcttatct cccagcaggc ggcaaactgg
    95221 ctgtaagcca gccactgcaa ccagagataa aaatcgacgt catcacgatg ttcttcgcag
    95281 aactgacgca cttctggtga atccacgttc ttatacatct ctggccatgc aggccagccc
    95341 cagcgcattt cgtcctcttt cacttgctgg gcatgtagcg catcaaaggc tgcctgccag
    95401 aacaggctgt cgccctgctc tgcaacaaac tggcgaaacg cggccatctg ctcatcatca
    95461 cgttgcgcga aacctttcca cgccattcgt aatgctgtca tttttagggc ggtaaccgtg
    95521 gagtaatcga cccagtcggc atcgcgcgcc tgttgcagcg tctgttgcgt ggtcggcaac
    95581 tgccaccagg cctgagcctc ttcgctaaga tggaaatctt caacggcgtt aacgtcgata
    95641 taaatcacat tcagccaacg gcgagaagac gggctgtatg ggctggcgct ctccggattt
    95701 gccggataga gcgcatgaat cgggttcagg ccaatgaacg acccgccacg ttttgccaca
    95761 tccaccagca tcgctttgag atcgccaaaa tccccaatac cccagttttt ttccgatcgc
    95821 agcgtataaa gctgaacgca ggcaccccac agcttttgtt tattcagcaa agcctgcggt
    95881 tcgtaacaac gtttcggggc gacaatcacc cggcaatgcg cgcgctggtc gtcctgggtg
    95941 agtgttagcg tgtgataacc ttccggcagc ttcgtcggta gattgaacgc tttgcccccc
    96001 gttacatggc ctttgtactg cgttccttct tcggtggtca gctgccagct atattcgccg
    96061 ctgccctcca ccaccatcgg catttttttg ccgctggtat aaaccatgac attcgggact
    96121 ggcgttaccg ccactttcgt ggcggtacgt tgatgcatcg cgtcaagcaa acgccgtttg
    96181 gtttcggcgc taatcgactg cggtttaccg tgggcattga tgtaattggg gctaatcccc
    96241 gccgccagcg cggcattatc cagacgtttg ctttccatcg agcttcctta gcgttttgcc
    96301 tgccagatac gagcctgata atcgcgaata gagcgatccg agctaaacat accgcagcgg
    96361 gcggtattga ggatcgccgc gcgagtccag gcctcctggt cgcggtacag cacatccacc
    96421 tgcttttgtg cctcaacata ggctgcgaaa tccgccatca ccagatacgg atcgccgccc
    96481 tgtttgccga tactgtgcag catctggtcg aaggcatgct tatcgccgtc gctgtattta
    96541 ccgctttcca gctctttcaa tactgcgtcc agcaccttat ctttcttccg ccatttcacc
    96601 gggtcgtagc ctttggccag aatagccttc acttgttcca cggtatgacc aaaaataaag
    96661 atattttctt caccgacttt ctcggcgatt tcaacgttcg caccatccag cgtaccgaca
    96721 gtaagcgcac cattgagcgc cagtttcata ttgccggtac cggaagcttc tttacctgca
    96781 gtcgaaattt gttcggagat atccgccgcc gggatcagtt tttccgccgc cgaaacgcaa
    96841 taatccggca ggaacaccac cttcaactta tcgccaacca gtggatcgtt gttgatcacg
    96901 tcagccactt tgttgatcgc aaagataata ttcttcgcca ggtagtagcc cggtgccgct
    96961 ttcgcgccga agaggaagac gcgcggtacg cgatcagcct gcaggttttc acgaatttct
    97021 ttgtacaacg ccagaatatg cagcagattc aggtgctggc gtttgtactc atgcagacgt
    97081 ttgatctgaa tatcgaaaat cgcctgtgga ttgatctcaa taccggtacg aactttcaca
    97141 aactccgcca gacggacttt attcgcctgc ttgatctcgc gatattgctg acggaatttc
    97201 gcatcatcag cgaatttttc cagattgatc agctgatcga gatcgttagc ccactctttt
    97261 tgcagtgatt tatccaacag agccgccagt gcagggttgc actgtttgat ccagcgacgt
    97321 ggggtaatac cgttggtgac gttatggaat ttgttcggcc atagctggtg atattccggg
    97381 aacagatctt tcaccaccag atccgagtgc agcgccgcaa caccgttcac cgcgaaaccg
    97441 ccaaccacac acaggttcgc catatgcact tgtttatcgt gcaccaccgc cagtttggcc
    97501 cacacttttt catcgcccgg ccaggttttc tccaccagcg ttttaaagcg agtattaatt
    97561 tcgttaataa tctgcatatg gcgcggcagt aagcctttca ccagtttcac atcccagcgt
    97621 tccagcgctt ctggcatcag ggtatggttg gtgtaagcga aagttttgct ggtgatggcc
    97681 caggcgtcat cccagctcat ctggtgctca tcggtcagca cgcgcagcag ttctggaatc
    97741 gcgatagttg gatgggtatc gttcagctga ataacttcgt aatccgccag ttcgtgcagt
    97801 ttacgccccg ccagatgatg gcgacgcaaa atatccgcta ccgaacaggc acactggaag
    97861 tattgctgca tcaggcgcag ctttttaccg gcagtatggt tgtcgtttgg atagagaact
    97921 ttggtcagtt tttccgcatt gatgccttgc tgttcggcgc gcaggaaatc accgtcgtta
    97981 aatttagtta gatcaaacgg atgcgcatgc gtcgcctgcc acagacgcag cggctgcgcc
    98041 acgccattac gatagccgac aacggggaga tcccacgctt gaccggtaat ggtaaactcc
    98101 ggctcccagc gtccgtcttt catcacttta ccgccaatcc ctacctgcac atccagtgct
    98161 tcgttgtggc ggaaccacgg gtagttactg cgatgccagt catccggcgc ttcaacctgt
    98221 ttgccatcga caaaagactg gcggaacaaa ccatattgat agttcagacc gtaacccgtc
    98281 gcagactgac cgacagttgc cattgagtcg aggaagcacg ccgccagacg tcccagacca
    98341 ccgttaccca gcgccgggtc gatctcttct tccagcaggt cagtcaggtt gatgtcataa
    98401 gccttcaacg aatcctgtac atcctgatac cagccgagat tcaacaggtt gttgcccgtc
    98461 agacgaccaa tcaaaaactc cattgagatg tagttaacat gtcgctgatt cgccaccggt
    98521 ttggcgaatg gctgagcacg cagcatttcg gccagtgctt cactcactgc cagccaccac
    98581 tggcgaggag tcatttcagc cgcagaattt aagccataac gctgccactg acgtgaaagc
    98641 gcttcctgaa attgcttatc gttaaaaata ggttgtgaca taggagttcc acttttctta
    98701 gattttcaac acaacgttat cgctagtttg ccaggctcga tgttgacctt cctcatcctg
    98761 cgggggatta ggcagggagg agttgcgggg atgagcaagg aaatgtgatc tcgaccactt
    98821 aaagctagtg caaaccacag gattagcagc aaatcaatgc catacagcgc agaaaatctg
    98881 tatctaagtg caaaaaatgg ccgttgcgta ttttcaaaaa gcggaaggta actctataaa
    98941 ttaagtaaag gagtgaaaca gtttcacaag taaaatatcc agtgtgctcc atctcattct
    99001 taatagattt attaagatca tctttttaga tggcactttc atcaggaatg aagaagaaac
    99061 ccttgcttaa atgaatctga t
//
LOCUS       DAETPZ010000003        86114 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.003, whole genome shotgun sequence.
ACCESSION   DAETPZ010000003 DAETPZ010000000
VERSION     DAETPZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 86114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.003"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..151)
                     /locus_tag="K0W59_000248"
     CDS             complement(<1..151)
                     /locus_tag="K0W59_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3021430.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQP"
     gene            complement(202..1803)
                     /locus_tag="K0W59_000249"
     CDS             complement(202..1803)
                     /locus_tag="K0W59_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBI3021431.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYMFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHG"
     gene            complement(1787..2143)
                     /locus_tag="K0W59_000250"
     CDS             complement(1787..2143)
                     /locus_tag="K0W59_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBI3021432.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSTKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            complement(2266..2448)
                     /locus_tag="K0W59_000251"
     CDS             complement(2266..2448)
                     /locus_tag="K0W59_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3021433.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(2432..2872)
                     /locus_tag="K0W59_000252"
     CDS             complement(2432..2872)
                     /locus_tag="K0W59_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBI3021434.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(2872..3168)
                     /locus_tag="K0W59_000253"
     CDS             complement(2872..3168)
                     /locus_tag="K0W59_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HBI3021435.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            complement(3165..3503)
                     /locus_tag="K0W59_000254"
     CDS             complement(3165..3503)
                     /locus_tag="K0W59_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBI3021436.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(3500..4711)
                     /locus_tag="K0W59_000255"
     CDS             complement(3500..4711)
                     /locus_tag="K0W59_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBI3021437.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLSAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(4713..5285)
                     /locus_tag="K0W59_000256"
     CDS             complement(4713..5285)
                     /locus_tag="K0W59_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBI3021438.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFSFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPREDNRRWWADLAGV"
     gene            complement(5325..6482)
                     /locus_tag="K0W59_000257"
     CDS             complement(5325..6482)
                     /locus_tag="K0W59_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBI3021439.1"
                     /translation="MQRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGNTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPEDDD"
     gene            complement(6785..6946)
                     /locus_tag="K0W59_000258"
     CDS             complement(6785..6946)
                     /locus_tag="K0W59_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HBI3021440.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(6956..7261)
                     /locus_tag="K0W59_000259"
     CDS             complement(6956..7261)
                     /locus_tag="K0W59_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3021441.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(7258..7536)
                     /locus_tag="K0W59_000260"
     CDS             complement(7258..7536)
                     /locus_tag="K0W59_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3021442.1"
                     /translation="MHTSGKFNKSLKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(7816..9564)
                     /locus_tag="K0W59_000261"
     CDS             complement(7816..9564)
                     /locus_tag="K0W59_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBI3021443.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRPQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(9561..9860)
                     /locus_tag="K0W59_000262"
     CDS             complement(9561..9860)
                     /locus_tag="K0W59_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBI3021444.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(9866..10099)
                     /locus_tag="K0W59_000263"
     CDS             complement(9866..10099)
                     /locus_tag="K0W59_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3021445.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(10101..10321)
                     /locus_tag="K0W59_000264"
                     /pseudo
     CDS             complement(10101..10321)
                     /locus_tag="K0W59_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10314..10535)
                     /locus_tag="K0W59_000265"
     CDS             complement(10314..10535)
                     /locus_tag="K0W59_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3021446.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(10525..10725)
                     /locus_tag="K0W59_000266"
     CDS             complement(10525..10725)
                     /locus_tag="K0W59_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HBI3021447.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSTGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            complement(10734..11480)
                     /locus_tag="K0W59_000267"
     CDS             complement(10734..11480)
                     /locus_tag="K0W59_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3021448.1"
                     /translation="MKIEYTPERGRGFVRPGETGKPQNWGFSGIKKAAPKWSRLSEQI
                     TRCAVCVCDPKHKHGDDSRYQAGGQCNQSGSVRCHNCEVLSFDEYSLRYFSRAKEHGA
                     NRSDSCSFFLRRLFRAGDVFSVDSSLVIVCACKAMRRSTSHHGADSFYPLGSMPRCCN
                     SLRIIRFIQAARESSPSCCCAFSIFSRKSGSKRNWNGGLPRLSFLCVDTLITPHVMYL
                     CVMTHYTQSTQKAKPRTVGAVTGLLTNNVN"
     gene            complement(11538..11726)
                     /locus_tag="K0W59_000268"
     CDS             complement(11538..11726)
                     /locus_tag="K0W59_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBI3021449.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(12101..13330)
                     /locus_tag="K0W59_000269"
     CDS             complement(12101..13330)
                     /locus_tag="K0W59_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBI3021450.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            13579..14700
                     /gene="roxA"
                     /locus_tag="K0W59_000270"
                     /gene_synonym="ycfD"
     CDS             13579..14700
                     /gene="roxA"
                     /locus_tag="K0W59_000270"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HBI3021451.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(14749..15975)
                     /gene="pepT"
                     /locus_tag="K0W59_000271"
     CDS             complement(14749..15975)
                     /gene="pepT"
                     /locus_tag="K0W59_000271"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBI3021452.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTNGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            16225..17361
                     /gene="potA"
                     /locus_tag="K0W59_000272"
     CDS             16225..17361
                     /gene="potA"
                     /locus_tag="K0W59_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HBI3021453.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            17345..18202
                     /gene="potB"
                     /locus_tag="K0W59_000273"
     CDS             17345..18202
                     /gene="potB"
                     /locus_tag="K0W59_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HBI3021454.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            18199..18993
                     /gene="potC"
                     /locus_tag="K0W59_000274"
     CDS             18199..18993
                     /gene="potC"
                     /locus_tag="K0W59_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HBI3021455.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            18990..20036
                     /gene="potD"
                     /locus_tag="K0W59_000275"
     CDS             18990..20036
                     /gene="potD"
                     /locus_tag="K0W59_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HBI3021456.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSQLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(20192..21013)
                     /gene="cobB"
                     /locus_tag="K0W59_000276"
     CDS             complement(20192..21013)
                     /gene="cobB"
                     /locus_tag="K0W59_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBI3021457.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(21029..21940)
                     /gene="nagK"
                     /locus_tag="K0W59_000277"
     CDS             complement(21029..21940)
                     /gene="nagK"
                     /locus_tag="K0W59_000277"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBI3021458.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWEDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(21969..23213)
                     /gene="lolE"
                     /locus_tag="K0W59_000278"
     CDS             complement(21969..23213)
                     /gene="lolE"
                     /locus_tag="K0W59_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HBI3021459.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(23213..23914)
                     /gene="lolD"
                     /locus_tag="K0W59_000279"
     CDS             complement(23213..23914)
                     /gene="lolD"
                     /locus_tag="K0W59_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HBI3021460.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(23907..25106)
                     /gene="lolC"
                     /locus_tag="K0W59_000280"
     CDS             complement(23907..25106)
                     /gene="lolC"
                     /locus_tag="K0W59_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HBI3021461.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVLSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            25368..26414
                     /locus_tag="K0W59_000281"
     CDS             25368..26414
                     /locus_tag="K0W59_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBI3021462.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIFSRLALPLFA
                     LFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLRRHLLMASLL
                     TVLAVGAWLFTISLLLSLVSTVVIMKLFYQYEQRFGMRSTSLLNVIGSNTIAIYTTHR
                     ILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKLSQRAFSDLL
                     FSPPSLPAAVSYSR"
     gene            26542..29988
                     /gene="mfd"
                     /locus_tag="K0W59_000282"
     CDS             26542..29988
                     /gene="mfd"
                     /locus_tag="K0W59_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBI3021463.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRYVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     SERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLNDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGMIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            30131..31093
                     /gene="ldtC"
                     /locus_tag="K0W59_000283"
                     /pseudo
     CDS             30131..31093
                     /gene="ldtC"
                     /locus_tag="K0W59_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(31176..31433)
                     /gene="bhsA"
                     /locus_tag="K0W59_000284"
     CDS             complement(31176..31433)
                     /gene="bhsA"
                     /locus_tag="K0W59_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HBI3021464.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            31674..32306
                     /gene="comR"
                     /locus_tag="K0W59_000285"
     CDS             31674..32306
                     /gene="comR"
                     /locus_tag="K0W59_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HBI3021465.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(32368..32907)
                     /locus_tag="K0W59_000286"
     CDS             complement(32368..32907)
                     /locus_tag="K0W59_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBI3021466.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(33134..34438)
                     /gene="ndh"
                     /locus_tag="K0W59_000287"
     CDS             complement(33134..34438)
                     /gene="ndh"
                     /locus_tag="K0W59_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HBI3021467.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(34839..35381)
                     /locus_tag="K0W59_000288"
     CDS             complement(34839..35381)
                     /locus_tag="K0W59_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI3021468.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(35404..36429)
                     /gene="nagZ"
                     /locus_tag="K0W59_000289"
     CDS             complement(35404..36429)
                     /gene="nagZ"
                     /locus_tag="K0W59_000289"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HBI3021469.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(36440..37264)
                     /gene="thiK"
                     /locus_tag="K0W59_000290"
     CDS             complement(36440..37264)
                     /gene="thiK"
                     /locus_tag="K0W59_000290"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HBI3021470.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(37245..37886)
                     /gene="lpoB"
                     /locus_tag="K0W59_000291"
     CDS             complement(37245..37886)
                     /gene="lpoB"
                     /locus_tag="K0W59_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HBI3021471.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(37901..38278)
                     /locus_tag="K0W59_000292"
     CDS             complement(37901..38278)
                     /locus_tag="K0W59_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HBI3021472.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(38281..38640)
                     /gene="hinT"
                     /locus_tag="K0W59_000293"
     CDS             complement(38281..38640)
                     /gene="hinT"
                     /locus_tag="K0W59_000293"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HBI3021473.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            38980..39804
                     /locus_tag="K0W59_000294"
     CDS             38980..39804
                     /locus_tag="K0W59_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBI3021474.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPT"
     gene            39808..41169
                     /gene="fhuE"
                     /locus_tag="K0W59_000295"
                     /pseudo
     CDS             39808..41169
                     /gene="fhuE"
                     /locus_tag="K0W59_000295"
                     /inference="COORDINATES: protein motif:HMM:NF007447.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(41229..42662)
                     /gene="ptsG"
                     /locus_tag="K0W59_000296"
     CDS             complement(41229..42662)
                     /gene="ptsG"
                     /locus_tag="K0W59_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HBI3021475.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(42957..43754)
                     /locus_tag="K0W59_000297"
     CDS             complement(42957..43754)
                     /locus_tag="K0W59_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBI3021476.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLCDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(43765..44769)
                     /gene="holB"
                     /locus_tag="K0W59_000298"
     CDS             complement(43765..44769)
                     /gene="holB"
                     /locus_tag="K0W59_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBI3021477.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWVATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(44766..45407)
                     /locus_tag="K0W59_000299"
     CDS             complement(44766..45407)
                     /locus_tag="K0W59_000299"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBI3021478.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(45397..46419)
                     /gene="yceG"
                     /locus_tag="K0W59_000300"
     CDS             complement(45397..46419)
                     /gene="yceG"
                     /locus_tag="K0W59_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HBI3021479.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(46422..47231)
                     /gene="pabC"
                     /locus_tag="K0W59_000301"
     CDS             complement(46422..47231)
                     /gene="pabC"
                     /locus_tag="K0W59_000301"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HBI3021480.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(47351..48592)
                     /gene="fabF"
                     /locus_tag="K0W59_000302"
     CDS             complement(47351..48592)
                     /gene="fabF"
                     /locus_tag="K0W59_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBI3021481.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFSCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(48679..48915)
                     /gene="acpP"
                     /locus_tag="K0W59_000303"
     CDS             complement(48679..48915)
                     /gene="acpP"
                     /locus_tag="K0W59_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBI3021482.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(49126..49860)
                     /gene="fabG"
                     /locus_tag="K0W59_000304"
     CDS             complement(49126..49860)
                     /gene="fabG"
                     /locus_tag="K0W59_000304"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HBI3021483.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(49873..50802)
                     /gene="fabD"
                     /locus_tag="K0W59_000305"
     CDS             complement(49873..50802)
                     /gene="fabD"
                     /locus_tag="K0W59_000305"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBI3021484.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(50818..51771)
                     /gene="fabH"
                     /locus_tag="K0W59_000306"
     CDS             complement(50818..51771)
                     /gene="fabH"
                     /locus_tag="K0W59_000306"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HBI3021485.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLGRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(51839..52909)
                     /gene="plsX"
                     /locus_tag="K0W59_000307"
     CDS             complement(51839..52909)
                     /gene="plsX"
                     /locus_tag="K0W59_000307"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBI3021486.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(52990..53163)
                     /gene="rpmF"
                     /locus_tag="K0W59_000308"
     CDS             complement(52990..53163)
                     /gene="rpmF"
                     /locus_tag="K0W59_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBI3021487.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(53215..53736)
                     /gene="yceD"
                     /locus_tag="K0W59_000309"
     CDS             complement(53215..53736)
                     /gene="yceD"
                     /locus_tag="K0W59_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HBI3021488.1"
                     /translation="MQKVKFPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            53935..54519
                     /gene="yceF"
                     /locus_tag="K0W59_000310"
     CDS             53935..54519
                     /gene="yceF"
                     /locus_tag="K0W59_000310"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HBI3021489.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(54631..55590)
                     /gene="rluC"
                     /locus_tag="K0W59_000311"
     CDS             complement(54631..55590)
                     /gene="rluC"
                     /locus_tag="K0W59_000311"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HBI3021490.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            56163..59348
                     /gene="rne"
                     /locus_tag="K0W59_000312"
     CDS             56163..59348
                     /gene="rne"
                     /locus_tag="K0W59_000312"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HBI3021491.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGNDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(59545..60498)
                     /gene="flgL"
                     /locus_tag="K0W59_000313"
     CDS             complement(59545..60498)
                     /gene="flgL"
                     /locus_tag="K0W59_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBI3021492.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRTELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(60510..62152)
                     /gene="flgK"
                     /locus_tag="K0W59_000314"
                     /pseudo
     CDS             complement(60510..62152)
                     /gene="flgK"
                     /locus_tag="K0W59_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(62218..63159)
                     /gene="flgJ"
                     /locus_tag="K0W59_000315"
     CDS             complement(62218..63159)
                     /gene="flgJ"
                     /locus_tag="K0W59_000315"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HBI3021493.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDQVSKTYSMNIDNLF"
     gene            complement(63159..64256)
                     /gene="flgI"
                     /locus_tag="K0W59_000316"
     CDS             complement(63159..64256)
                     /gene="flgI"
                     /locus_tag="K0W59_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HBI3021494.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAVVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(64268..64966)
                     /gene="flgH"
                     /locus_tag="K0W59_000317"
     CDS             complement(64268..64966)
                     /gene="flgH"
                     /locus_tag="K0W59_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HBI3021495.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(65019..65801)
                     /gene="flgG"
                     /locus_tag="K0W59_000318"
     CDS             complement(65019..65801)
                     /gene="flgG"
                     /locus_tag="K0W59_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HBI3021496.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(65939..66690)
                     /gene="flgF"
                     /locus_tag="K0W59_000319"
                     /pseudo
     CDS             complement(65939..66690)
                     /gene="flgF"
                     /locus_tag="K0W59_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(66710..67918)
                     /gene="flgE"
                     /locus_tag="K0W59_000320"
     CDS             complement(66710..67918)
                     /gene="flgE"
                     /locus_tag="K0W59_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HBI3021497.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(67943..68638)
                     /gene="flgD"
                     /locus_tag="K0W59_000321"
     CDS             complement(67943..68638)
                     /gene="flgD"
                     /locus_tag="K0W59_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HBI3021498.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(68650..69053)
                     /gene="flgC"
                     /locus_tag="K0W59_000322"
                     /pseudo
     CDS             complement(68650..69053)
                     /gene="flgC"
                     /locus_tag="K0W59_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(69057..69230)
                     /locus_tag="K0W59_000323"
     CDS             complement(69057..69230)
                     /locus_tag="K0W59_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgB"
                     /protein_id="HBI3021499.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVTQFADNSLQYQMSLSALSG
                     QIKGMMNVLQSGN"
     gene            69385..70044
                     /gene="flgA"
                     /locus_tag="K0W59_000324"
     CDS             69385..70044
                     /gene="flgA"
                     /locus_tag="K0W59_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HBI3021500.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            70120..70413
                     /gene="flgM"
                     /locus_tag="K0W59_000325"
     CDS             70120..70413
                     /gene="flgM"
                     /locus_tag="K0W59_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HBI3021501.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            70418..70834
                     /gene="flgN"
                     /locus_tag="K0W59_000326"
     CDS             70418..70834
                     /gene="flgN"
                     /locus_tag="K0W59_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HBI3021502.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDHEQQHLSMGQINCSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(70874..72409)
                     /gene="murJ"
                     /locus_tag="K0W59_000327"
     CDS             complement(70874..72409)
                     /gene="murJ"
                     /locus_tag="K0W59_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBI3021503.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(72576..73442)
                     /locus_tag="K0W59_000328"
                     /pseudo
     CDS             complement(72576..73442)
                     /locus_tag="K0W59_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(73444..74091)
                     /locus_tag="K0W59_000329"
     CDS             complement(73444..74091)
                     /locus_tag="K0W59_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HBI3021504.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYLLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTLGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(74102..74686)
                     /gene="rimJ"
                     /locus_tag="K0W59_000330"
     CDS             complement(74102..74686)
                     /gene="rimJ"
                     /locus_tag="K0W59_000330"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HBI3021505.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HTCYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            74922..76130
                     /gene="mdtH"
                     /locus_tag="K0W59_000331"
     CDS             74922..76130
                     /gene="mdtH"
                     /locus_tag="K0W59_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HBI3021506.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSTHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            76194..76841
                     /gene="grxB"
                     /locus_tag="K0W59_000332"
     CDS             76194..76841
                     /gene="grxB"
                     /locus_tag="K0W59_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HBI3021507.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            76975..77535
                     /locus_tag="K0W59_000333"
     CDS             76975..77535
                     /locus_tag="K0W59_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBI3021508.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            77641..78687
                     /gene="pyrC"
                     /locus_tag="K0W59_000334"
     CDS             77641..78687
                     /gene="pyrC"
                     /locus_tag="K0W59_000334"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBI3021509.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLQTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHTRHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            78761..79006
                     /gene="dinI"
                     /locus_tag="K0W59_000335"
     CDS             78761..79006
                     /gene="dinI"
                     /locus_tag="K0W59_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HBI3021510.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEIPQETWESADDWFVSE"
     gene            79296..79550
                     /gene="bssS"
                     /locus_tag="K0W59_000336"
     CDS             79296..79550
                     /gene="bssS"
                     /locus_tag="K0W59_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HBI3021511.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            79665..80783
                     /gene="solA"
                     /locus_tag="K0W59_000337"
     CDS             79665..80783
                     /gene="solA"
                     /locus_tag="K0W59_000337"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HBI3021512.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            80831..80944
                     /locus_tag="K0W59_000338"
     CDS             80831..80944
                     /locus_tag="K0W59_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HBI3021513.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            81205..81771
                     /locus_tag="K0W59_000339"
     CDS             81205..81771
                     /locus_tag="K0W59_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HBI3021514.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            81775..82350
                     /locus_tag="K0W59_000340"
     CDS             81775..82350
                     /locus_tag="K0W59_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HBI3021515.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(82392..83444)
                     /locus_tag="K0W59_000341"
     CDS             complement(82392..83444)
                     /locus_tag="K0W59_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBI3021516.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            83669..84589
                     /gene="lpxL"
                     /locus_tag="K0W59_000342"
     CDS             83669..84589
                     /gene="lpxL"
                     /locus_tag="K0W59_000342"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HBI3021517.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            84761..84958
                     /locus_tag="K0W59_000343"
     CDS             84761..84958
                     /locus_tag="K0W59_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBI3021518.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            84982..>86114
                     /locus_tag="K0W59_000344"
     CDS             84982..>86114
                     /locus_tag="K0W59_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI3021519.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYWDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGIIKCSECGATIVLGGYALKRFGMYRCPNRSANR
                     CSAKAISRKQTDATILYMLALCDRFETENTDTIDSLKLQREDL"
BASE COUNT        20679 a        22906 c        21216 g        21313 t
ORIGIN      
        1 cgggctgaga ggcggtgtaa gtgaactgta gttgccatgt tttacggcaa tgagagcaga
       61 gatagcgctg atgtccggca gtgcttttgc cgttacgcac cacgccttca gtagcggagc
      121 aggaaggaca tctgatggaa atggaagcca cgcaagcacc ttaaaatcac catcatacac
      181 taaatcagta agttggcagc attacccatg ctctgcctgg aatgtaccaa acgccatcag
      241 cgccgccacg ctcgggtcta tcttgttcgg tgattttttc ttgttcggct tgatattggc
      301 gttcgcgtca ctctgcatca cgacgttact catcgaccag gacaacaccg gatcgccacg
      361 atgcacaatc accctgcggt taacaaaaac ttcgaacgat ttcgccgccg gactgaatct
      421 gaggtaggtt tgcgggaacg gctccacctc aaaaccagcc ccctgtaatt gcgtcctgag
      481 atgcgtggcg ttccacgtat caaagcccac cagcctgata ttaaatttct ccgcatcctg
      541 catgatgtca tctctgatcc ggtcgtaatc aatgcagtca cctggcgttg tgcgtatcca
      601 gcccgcttta gcccactggc gatagacagc gcggttttta ttggcggggt tctgtagctg
      661 gaactccggc agataatgac gggaaatcag cataatggtt ttaccgaccg gaaaggcata
      721 gcatacgctg gaaatatcgc tggttgatga taagtccagc cccgcgtaac actcctgacc
      781 gtgtaaatct tcctccgtga acgttccggc acactcagcc catgcaccat tacccatcca
      841 cggggtagtc ccctgacacc agatattaaa tcgctttgtc atcatctcca cccactgcga
      901 cggaataccc cgcgctttct ggatggttga ggccagtttt tcacgatcca cggaaacatc
      961 aatattaggg ttcgccttta tccacatcgc cggatcatca acctcgtttt cgtcgtccag
     1021 ttcgtaaatc agtacaaaaa ttgaatcgtt gacctcttcg ccgtccagga tctggcagca
     1081 ataatcatag tgctgtttac aggctgaaac gacgttactg cccgatgtgg taatggcaaa
     1141 taacagccct tcgggacgtg cgcccatccc cagctcaagc gcggaataaa cgccgttatc
     1201 ggggtgtagg tggtattcgt ccacgatgga aagactgggg tttgtcccct cgatggttgc
     1261 cgcttttgct gccagcggct ttaacaggct gttgcttttc gggtgtatca ccttatgcgc
     1321 ctgaatattc acccgcctgc gtaacggtcg ggataaaagg cacatctgac gcgcatcatc
     1381 aaacacgatc cgcgcctggt cacggctcac cgctgcggtg tagatatcct gctgaccgtt
     1441 ctccataatc agaaaccagt tagccagaat cgcggcggtc gtggatttcg catttttgcg
     1501 cggcacttcg ataaaggcgc tcgtgtattt gcgccgtccg gtggccttaa ccttaaagcc
     1561 gaggatgcac gcaaaggcga actgctgcca cggctccagc tcaatgggtc tgccacgcat
     1621 cggtcctttt acgtgcgggc acaccctgga aaaggcaata aaccgctcca caacctcacg
     1681 atcgaacatg taaagggggc ttttaaggtc cgaaaagtac cgtttaacag cctgttttaa
     1741 ccgtttacag gccggaattt tgcccgtttt tacgtcttct gcgtacttat tccaggcggt
     1801 caagctcgtc ctcttcttct gtttccggtg gatttttacg gcggcttatc gggtcaaaac
     1861 cgagcaagga ggccattttt atcatcactc tttcagcgtc ggattttgtg cttaatgcgg
     1921 ggtttctgct ctcgccgccc tgactgttaa caatgctgaa cccgcgcgcc gcaaggtctg
     1981 caacggcttt ccggtaaatg gagtagttga cgcaatacag ttccagattg ctccagtcgg
     2041 cgggggtcag gtctccccgt tccgcaagct gccgcgattt ttcccgccac tgcttcacgg
     2101 cgatatcatc caggtaggcg ggggctttcg gtggtcttgc catgcttatt tttcgtcaga
     2161 ttatttttca aaaaattccc gtgcataaaa atttggggag gcgttcggtg cccggcgggg
     2221 tcgggtttgt cctgaaaacg ccccccaccc cgtcatacag cctcatcagc gattgcggaa
     2281 acattccata acctcgcggt cacggtcggt taatcgcttc gctgtgatgc gttctgcgcg
     2341 tcctgacgct ttatctttat gccctgtttc ctgtgtcttc catgcgtcac gctgccttat
     2401 aagtccacgg ataaggcggt tttgttcccg ctcattcatc agcgtcatac atccagttat
     2461 tgcggttagc agcccgttct tcctcttcac gaaatccacc tgcggcacgc ttgctttttg
     2521 ttgccggatc aagccatttc gttttctggt tatgacacgc ctggcacaat ggttgatggt
     2581 tccattcggg ccagaagaga acatcatcac cgccattaat cgggataatg tgatccacca
     2641 ccacggcggg cgtatatatc cccttctcaa ggcatcgcac gcataacggg tttttactca
     2701 gatacatggc gcggtatttg tcccactgtc tggaatatcc acgcgcgcgg cggtgtcctc
     2761 gtctggcatc ctctgcacgc catgccgccc gcctgtgctc ctcacacttg ccggatttaa
     2821 cgcgcctgtt acagcctggt tctgtgcatc gtcttaatgg ttgccacggc atcagtacac
     2881 ccccacatca cggtaagccg tccagagtgc gcctatcgtc atgggtacgc gtgtttttgc
     2941 gttatccgcg acaatctggc gattttcata caggtgagcg ataaacatca tgcagccaat
     3001 ctttatggct ggcgtgaacg ccagcccgtc atcaaagcgc ctgcctatgt gtatctggca
     3061 cgcctcaagc gatgcggcaa tgtatccgct gattaactgg tcttcatcgt cgccatcgat
     3121 gcggcaatgg agtttcactt cttccagggt gataagttct tctgtcattt ttccgcgccc
     3181 tcacgacaaa gaatttcaag gcgtgtcctg gcggcatccg gcagcggctg cccgatgata
     3241 ttcagtacac gccccgccag cggccccgta ctgaccttta tccggctggt ggcgttgatg
     3301 tccttccggt accgtatcca gatccttacg gtaccaatcg ccagctctgc gcccgatgaa
     3361 atggcctcct tgctgctgac catgttcacg cttgcccaga gtgtgtgacc gtcctcccac
     3421 gtttcgagta tttcgccggt tatatccctg gtctgtttca gggtctgaat cgttatcctg
     3481 tcacgcagtc gccctatgtt cattccggtt tttctccctc gctgattttt acttcctgtt
     3541 tccatgcctg gctgaactca tcaccaccat cacgcggtga cagcccttca cgttcgcggg
     3601 cttcgttcgg acacataacg ccggatttta tgccgcgctc ataggtggca aagcgttcgc
     3661 caggtgtggc ccgtaacagg tccgcgctgt caaactccac ctgataccgg ataccaggta
     3721 caggcgaggc caccagcaag gcatttttta tctgctgctc aaagttcgcc agccacgggc
     3781 gcatcgtcat ggtgagaaat gcgcggcttg cctcgctgaa gttgctgtag gtgctgttgc
     3841 tgtattcctg caaaaaaatc ggcgagacgt tgaacattcg ggcgatgtct tcaatggtga
     3901 agcgacggga ggccagccat tcggcatcct gattactcat gcccagctgc tgatagctca
     3961 tccccccttc aaggatgggc gtttttccgg cgtttctggc ccctttgtag cgttccagtg
     4021 cggctaatgc ctgtttgccc ttcacgccgt ccagccattc gcctgacgtg ataacccctg
     4081 ccgccatcat gccatcacgc atcacgctcg cgccgtggcg ttgttgggcc agcccaagcc
     4141 ccagcgattc gcggcagatg gttacaggtg agcgccccat aaaaccgtca tccgtggagt
     4201 aacgaaggtg gagaacctcc cacggtaaat agttgcgggt gtttccggtg taggcgtcag
     4261 tgatgcagta gcgccagtta tgatcacctg tctgctccac gttcaccgac tgcggcgggt
     4321 aaggatgtaa agccgccgga aaaccatcac gcccccactg aataaccgca taagcattac
     4381 cacttaacag gcagtggcgg atcatcattc gcttaaactg gtagggtgtc tgccacgcgt
     4441 tcgggcgctc gttgaggatg tgatcgaccg gatgagaatc aagccattcg cgggcctcct
     4501 tccccttctc attgcgtacc aggtacaggt aacacggcat ggtagccacc gcctcagaga
     4561 tgaccgtgac ggcgttcatg acggcggaga gtgattccgc tgtcccagat gatacgtact
     4621 cacccgcccc cgtgttcgat gtgccggcca gcgccataaa ttcatcaagc gtcatgctgc
     4681 gctgctcttt ttttcgtctg aaaggccaca tatcacaccc ccgctaaatc cgcccaccag
     4741 cggcgattat cctcacgcgg cattttttcg gggtgctgct catacaggga acggcgggcc
     4801 agctccacgc cggaatcggg gtaagccggt accgatgtaa cggtaatttc gtacagttcc
     4861 gccaccagca cggtgcgcac gcatggatct gttgtggtat cccatacatc cttacgggag
     4921 cgaaagctaa agctcatccc cgagatgtcc ccacgcttaa ccagttcgat aacgtcgcgc
     4981 cctgtactgg tatccggtgg ggtcagttca aagcgtaacc ctgtttcgtc ctcttccagt
     5041 ttcagcgtgc cggaacgggt gcgccccagt aacatgctgt ggtcatgctc atacaggccg
     5101 cgaacgtcat tacccgccgc aagccattca gtaaacgccc cccgctggaa tttttcgtaa
     5161 aactcacccc ataacagttc tgaaagctta tcccagcgaa caacgtagcc cgtcagcgta
     5221 ccggctccgc tggtggtgat ttccgatgac cggatttcca tactcttcat aattttttca
     5281 cccataaaac actaaagggg ctttttagcc ccttctgtat gctgttaatc gtcgtcttcc
     5341 ggcagttcca gaatcttgat cgcgttcgaa tccaccacgc caccgcctaa atatttttgc
     5401 gtgaaaattt tgatgaatcc tggttctgtc aggttgtccg gtctggtgcg aacacctgtt
     5461 tcgtgatcaa caatgtagta accgcgtttg aagtcaccca gggcaataac gttatcaggc
     5521 ataaactcca gatattcgac cggaaggccc agcaacgtat caggatcacc cgcctgtaaa
     5581 cggtcgcgcc agatataatc accgttcgcg ttcttcacct tctgaagttt tgccgccgtc
     5641 gtggagttaa ccacccagac cgcgtttttg cggtattttt tacgtaatgc aaatttcagg
     5701 tcgatcagcg ggtccgcaga tgtccacgcc agagattcgg aaggtttaat tacctgcaac
     5761 gtaccaaaat cacgctcttt gtcgttcttc tctgcacggg gtacggataa aaaacctttt
     5821 gcttttttgt caccgtcgcc cacaaccaga tcgctttctt cggtttccgt gaaggtgttg
     5881 ccaatctcac ccgtcagcca tgaaaggatg tccacatcgg aaaaatccac gatttcctgt
     5941 gtggtgcgcg ggtacgcata gaccggatac agcttaatgc tcacctcgtt aatctgcggg
     6001 gtgctggtct gttcgcgtgc cttaccctct tcaccgtggt taacggtcgc accgccagcg
     6061 gaaacaagct gcttaaactc gttgctgctg attttcttca cggtacagat gcggcgcatg
     6121 gtggattcat ccgccagcat tcgcatgatt tcggtgttca gttcagggat aacggtataa
     6181 ccaccatcag cgggaacgcc tgtacttaat gcgcgggttt caccggtcag aatgtagttg
     6241 cgtagttctg cggggtcagt ggtctggctg cttttacctg gcttgctgcg ctcttcgtct
     6301 gcaatggctt caaggcggga aatgtcttta tcgagggatt cagctttagc gcgtaattcg
     6361 tcaaattttg cgccctcagc atcgttaaga ctgcggtttt ctttttcagc gttctccagc
     6421 atgtcgcgca tctgattttt aatggcggtt ttctgctggc gtaattcgat tattctctgc
     6481 ataaaaaagt cctggggtta agtaaggaac tccaggacgc ggcaaacact caaccgtttt
     6541 tcataaggaa atcagtaatc gcaccgatcg ttttcccgcc tggtaatgaa tattggcgag
     6601 cacattaaca ggcgggaaag tggccccagc gtcctggcac cacgggcgag aataatcatg
     6661 attcagttcg ggtaaaatat gccgatccgc tcagtgaaca acgtggaaca accacgaaca
     6721 aataatttac aaaaaataac aaaaagccgg attgctccgg ctgcgtggtg tggttctgat
     6781 attcctactc tgctaatttg ctcagcccca tgtctgcgta agtgcgattt actgcatttc
     6841 tcaggtctgc gtagttctcc gttggcggct ccggtgtgcc cttgctgctc tgttttacca
     6901 gcgggtcgcc caccagccgc ccgtaagctg caatttgtgc tgtcatattg ccacctcagt
     6961 gaagcgatcc ggcattgttc tttttcatct cttccatcat tttttcagtg agcattacgc
     7021 actgaaaccc catgtttttg atgtctacgg gtttcatgcc tttgtcagtg gatatcacag
     7081 gcagaatatc ctgatacaca tcgcggtcag ggtaactaag cacacaaagc gcccatgatg
     7141 ccacgggtga ggatccttcg tctgtttcaa cccaccagcc tggtgtagct gcgcttattt
     7201 gcagaatcgt gatccgtccg ttgtgcttgt aaaatttttc accaaatttt ggttcgctca
     7261 tgattccacc tctccggttt taacgttgat tgttgttacc tgttccgctt cggcaatctc
     7321 ccgctctgtc agcgtggcaa aatttgccgc cgtcgtggtc atgaatgcgc ttatcagttc
     7381 gggatgtgct ttcgcgtatc cttcccccgc gtggcggtct atcgttctga ttgccacctt
     7441 taaagcgtgc tctgtcatgt ctaacgcgcg atatttcggt tctgttctgt ctctgcgttt
     7501 tttgagtgat ttattaaatt tccctgaagt gtgcatattt atttttaccc cctcgtttaa
     7561 aaagttttga gttgtgcctc cccttgtcta ccttagtggc cctcatgcca gtaatggcgc
     7621 tgctttcagc ggggtagagt gcttttatcc actatctacc ccgtgtctac ctccctgtct
     7681 gattcaggta aaatcaggta gagagggtag atagtgggta gacagtaaaa aaaggctacc
     7741 tacctaactt aatgcactga attaaatgta tttttcttta ctcaggtaga cagggtagac
     7801 agcaattaca aaaaattata aaaacgcgtc gcactcgtct gttgttattg cgttagtctg
     7861 cgttactccc ttaactttcc gcgtaatata ttcatgtccg taaactttcg cggctggctt
     7921 catagccttg ccaaagtcat ttacgtttag cggtttgctc ctgcctgcgt acgccataaa
     7981 cgccagatag acgcggtaaa ggctgtttct ggtcgtgtac ttcactgaat cgccaccgcc
     8041 gcccatcatc aggccgcgtg cttcctccag aaaattcagg aactggcaaa actcaataac
     8101 cggatccgtc tgttgcttta ttgccagagc ttcatcaccg tcacgctgtt ccagtagtaa
     8161 agcccgtgct ttctcagagt cggtaaagtt cgccagcaat cggcggataa tgacagggat
     8221 ttcagccgca atcttttccg gtagctccct gtctttttcg gcctcactaa cgatattgtc
     8281 gaaacggaaa atcacgcgac gacgtgccac acctccggcc cgttcggtga atatcatcgg
     8341 gttattgttg gtcgccagca ccaccgccct gattacagcc gtgaaacgct tttcgtattt
     8401 cgggttaatt tccacggggt caccgcccgt gattttcttg atgcccgttc cttcgcctgt
     8461 atatttcggc tggtcagcca ggacgataag acgactcccg acaacctgcg gacgtccacc
     8521 agcatcatca agcgatgtca tttcagcgct taccgtgttc tgtttccctg ccagaaggct
     8581 ggctatgtgt gtgaatgtac ttttaccgct cccgccgtct ccggtggcct caataaacat
     8641 ctgccagtcg taccggttcg ccataatcat gtacagcgcg gcacatatac gcatcatctt
     8701 gcgctggtct tttccggctg cgtgctcaag ccatttatga aagtttagcg cgttatcgcg
     8761 gatgttctcc cctggtgctg gtggcgtgta ctcaatgccg ttgtgcgtgg tgatccagtt
     8821 ctccggcgtg tgcggggaaa attcccccgt tttcaggtca agcgcaccat tggcgaacgg
     8881 cagcaaatcg ccggacggct cgcccattgg ttcggcaata acttttaacg cttccacggc
     8941 gttattgatt acgcgcttgc tgaaagtggc cctgtgctct gaatagatcg ccaccatttc
     9001 gcggctaagt tccattgtgc tgaccggaca ccataccccg ccgcgccata cgtgaacgat
     9061 ttcactttcc tgatgcacac aaacgccatc aaagcgctcg gcaagcagct gcgcgcgctc
     9121 actgtctgcc atttgtgcca gttgcgtatt tttctgctcc ggcatggtaa gccctgcggc
     9181 aatattttca cgctcagcat tcagatagcg ccgccagttt tcacacttct ggacgtgcat
     9241 tccttcgggg taaaaatttg cgtcctgaat atctgccgcc gccagcttct gaccaatctt
     9301 tttggtctcc accaaatcca gttctccggc ctggtacagc cttacgcgtt tttttccatc
     9361 cggaacaatt ttcagtgcat caagttcggc aagctgattt ggcccaagcc acacaggcgg
     9421 cacattatcg ccggatgcgg ggccatcctg ctcctgccat tgtttcgcgt gcgaccaggc
     9481 atcactaccc gcaaagataa ttacctctgt gtctttgtgt tttattccgc gtggctgttt
     9541 ttttacgttc ggtgccagtt tcatttttta cccctgaata cgttaagcat cttttttatt
     9601 tcctgaatat tggcgcgtgc tttctccctg ctggttggtt tactgcgggg cgctgcctgt
     9661 accagcgtga aatcacgccg gaattgataa acaggcatca cgcaatcata ttcgtaacct
     9721 tcacggcggt aggtgatgcg ccgttctgcc acgcctttaa tcgttaccat gccgccgtac
     9781 tggtcgcggt aaatatcacc gcgcgtaaat ttagggtgag tgttgccact ggcagttaag
     9841 ccagaatatt taagtttcat tatttttatt ctccggtgtg ctgttcttta tatctgtcgt
     9901 gcaatagatc tatttcttgc agttccatta ttacaggctc aagaagcgtt attaatgccg
     9961 tggcaattct tgatttttgt ttgtcgcgtt cattgtcgcc aagtgtttca agccatatgc
    10021 gcaatatttc cagcatgttt tcactgtgag aaagtgcaag aaatgcgcgg tctattgttt
    10081 cgtggtaaat atcacgcata ttaatccccg tccgtcgttt ttcttaaaat aacctctgtc
    10141 acgaaatcag cataatcggc ggcgatattc agtacatcaa gcccgttgat ttatattctc
    10201 tcgtggaaag taagaaaaaa gccgctctaa taagttctgg cattgacgaa agcgcatcag
    10261 ccgcatcatc aggaacgccg gaaaattcct gtttcaggga attaaaacga tcatcacgca
    10321 taaccccccc cattttcaca atcggcaaca agaatatttt tagcgtcatt cagtgaccgc
    10381 gttgcgatgt atcggataca ttccagggcg aactgtgtcc ggtctttctg ttcttctccc
    10441 tgcgcaagct ctgccgtgca ttcaatatca ataagcgcgt gcatcagcgt agtgagtgcg
    10501 gcggctgctg cgttcggtgt ggttttattg tgcataggtt tccccctgac gaatacgggc
    10561 ggcgaataca gcaacacaac cggacgggca acggctacgc gcttcgcgtt ccgtccaagc
    10621 ggttacgtgg atgatttggg attctccggt actcagtgcc agaaaacgcc acacaaaggc
    10681 cgtttgtgtg tgtacaaggt gtggtatatg atttacggca accataacgg ctcctagttt
    10741 acgttgttgg ttagaagccc cgttactgct cctacagtgc ggggctttgc tttttgtgtt
    10801 gattgtgtgt aatgtgtcat tacacataaa tacattacat gaggtgtaat taacgtgtca
    10861 acacacaaaa acgaaaggcg tggtaatcct ccgttccagt tccgtttaga tcctgactta
    10921 cgggaaaaga tggaaaaggc acaacaacag gacggcgatg attctcttgc tgcctggata
    10981 aagcgaataa tccgcaaaga attacagcaa cgcggcatag aacccaaagg ataaaaacta
    11041 tcagcgccgt ggtgtgaggt actacggcgc attgctttac aggcgcacac aatgaccaac
    11101 gaagaatcaa ccgaaaaaac atcacctgca cgaaaaagac gacgcagaaa gaaagagcat
    11161 gaatcagaac gattcgcgcc gtgttctttt gcccttgaga agtaccttaa agaatactca
    11221 tcaaaggaga gaacttcgca gttatggcaa cgcactgaac cagactgatt gcattgccca
    11281 ccagcctgat agcggctatc atccccgtgc ttatgtttgg gatcacacac acaaacggcg
    11341 cagcgggtta tctgttcaga aaggcggctc catttcgggg ccgctttttt tatgcctgaa
    11401 aaaccccaat tttgtggttt tccagtttca ccagggcgaa cgaatccccg cccacgttct
    11461 ggcgtatatt caatcttcat ggttatagct ctgtgttcag atgattgatg tgtggcggct
    11521 gcgtgccgcc agcgtgatta atgaactgcc ttgcagctat ccttccaggc cagaacctcg
    11581 gataaagacc agccaacgga acgaccgcca agtttacgac gtgatgggaa ttgtccggcc
    11641 ttttccaggc ggtagcggca tgagcggcta aggcctgtta gcttttcgca ttctttttca
    11701 cgtataaacc gatcagtgct taacactatt gccccctttc gtttcttaaa gagttatttc
    11761 gtgttctatt gcgttgggat gtgtctgatt gtgtcaggat gattcagagt tggcaaatgt
    11821 tgaggccgta tggtttacag aaagaggaac aatcaggata aaatcattta aattcatgtt
    11881 aatacaaagg cataaaatat tgtttcatgc cttttttctc gctatttaaa gagtgattcg
    11941 ctagtgtata aaaaaccagt aacacgttaa aaatcagcta cttataaatc cgtacacttt
    12001 ttcacctctt gttcgtagtt gttccgcatt gttgctcatt gttgcacgtt gtatctgttc
    12061 gcatatccag tatgcgcata ctgaaaaaac acgaaaaaaa ttattttctt ctggctactg
    12121 gtagcgtggt tacgttttca tgtgttcccg ccagtatccc taaccgctcc gtccacatat
    12181 ccagcgcatt gcgtttagca tccagataac gggaatgatt ataaactcgc tgcattcctg
    12241 gcatctggtg gcctgtaagc tgctccacga cgtgaggatc cacgcctaaa tcgttcagca
    12301 tcgttgtaaa ggtgcgccgg atgtcatgta gtgaccagtg agggtgattt agcctcctgt
    12361 gcgctaatct gccgtactgc gacacgcttg tttcctgttt cacttccccc agcaataagc
    12421 ccgtgtgcct gttctgctcc accagctgcg tgacgaacgg cagtattgct tccggtatgg
    12481 gccggaatat tgccaccttc gttttgctgt gttctttcgg cacggtccag agcatttcgg
    12541 taaaatccca ctcgctgatc tccgataacc tcagttctac cgtccggcat ccgaacacaa
    12601 tcaggaggcg gattaacgcg atgtagtagg gggagaatat ttttttgtcc agtgcctgca
    12661 ataattcgcc cagttctttg gtgcttaaga cacgctcgct tatatccggt tttttcccaa
    12721 cgtccgccac actcatatca tcaagaacgt tgctgattgc atagcgccgc ctccggcaga
    12781 acttaagcgc ctgtttgcac gtctgtagca agaatccggc agtaacaggc gttcgctttg
    12841 ccacctggtc aaaacaggcc agccagtgcc gtagctcgca tttatccagc ggcatagcac
    12901 caatgtgctg tattacgtga ttattaaggc gctttttcag ggcggcataa tccacgcggt
    12961 tttcctttgc gtacgactca agccagtagg tgagcgcatc gccaaccgtt accggcttta
    13021 acgcttcctg tacggtgtaa ttaagctcat gacgtggatt tttcccctct gccagccatg
    13081 cgcgacactg ggcggctttt tccctggctg atttcaggct cagatcagga taatttccca
    13141 gcttaatgcg ttccggtggt gccccccttc ccgttccggc cctgtaagtg aaataccagg
    13201 ttaaaaggcc actggttgaa tgcctgacgc tcaggtttcc accgtcatta agaaaggctg
    13261 ttttttgggc gggtgtgccg ttgattttcc tcagctgtgt atcgctcagt ttgttaagtg
    13321 ctctgctcat aattttgatt ccggtcatca caattaacta caccattaac tacaccgatc
    13381 gttgcacaaa gggctacaac gtgaaacaag ctggaacaag gaaaatcaca aatctgttta
    13441 taatcaaaaa cataatgaac aatctgaaac attatgaaac ggcaaaaaac actaattgat
    13501 aaaatatgtc catacttcac gcattacgtt aagcatccgt tataatcggt tgcagatacc
    13561 agcctgtgga tgcttaacat ggaataccaa ctcactctta actggcccga ttttcttgaa
    13621 cgtcactggc agaaacgccc ggtggtgtta aaacgcggct ttaataattt tattgacccg
    13681 atctctccag acgagttggc aggtctggcg atggaaagcg aagttgacag tcgactggtc
    13741 agtcaccagg atggcaaatg gcaggtcagc cacggcccgt tcgaaagcta cgatcatctc
    13801 ggtgaaacca actggtcatt actggtacag gcagtaaacc actggcatga gccgaccgcc
    13861 gcgctgatgc gaccgttccg tgaactaccg gactggcgta ttgatgatct gatgatttct
    13921 ttttctgtac ctggcggcgg cgtcggcccg catctcgatc agtacgacgt gtttatcatt
    13981 cagggtaccg gacgtcgtcg ctggcgagtg ggcgaaaagc tgcaaatgaa acagcactgc
    14041 ccacatccag atctgttaca ggtcgatccg ttcgaagcca tcatcgatga agagctggag
    14101 cctggtgata ttctttatat tccgccagga ttcccgcatg aaggctacgc gctggaaaat
    14161 gcgatgaact attccgtggg ttttcgcgcg ccaaatacgc gggaattaat tagcggattt
    14221 gccgattatg tgctgcaacg tgaactgggc ggcaactact acagcgatcc ggatgttcca
    14281 cctcgcgctc atcctgcgga tgttctgccg caagagatgg ataaactgcg tgagatgatg
    14341 ctcgaattga tcaaccagcc ggaacacttt aagcaatggt ttggcgagtt tatatcccag
    14401 tcacgtcatg aactggatat cgcgccgccg gaaccgcctt atcagccaga tgaaatctac
    14461 gatgcactga aacaaggtga tgtgctggtg cgcctgggtg gtctgcgcgt attgcgcatt
    14521 ggcgacgacg tgtatgccaa tggtgagaag atcgattccc cgcaccgtcc ggcactggat
    14581 gcactcgcca gcaacattgc gctgactgcg gagaattttg gcgatgcgct ggaagatccg
    14641 tcattcctcg cgatgctcgc ggcgctggtc aatagcgggt attggttctt cgaagggtaa
    14701 gtttggattt aagccggatg cagcatcaca tgccgcatcc cttttcgctt acttccgttg
    14761 cgccgttaac tcggcaatac ggacgatcac ctgcaccgct ttttccatac cttccagaat
    14821 cacaaactca tgcttaccat gatagttgta accgccagtg aacaggttcg ggcaaggtaa
    14881 tcccataaac gacaactgcg cgccgttggt accgccgcgg atcggtttca gttccggttc
    14941 aatatcgcag tcacgcatcg cctgctgggc gatatcgaga atatgcggat gctcaaccac
    15001 tttctcgcgc atattgtagt aactgtcttc aatcaccaat tcaatgtagc aatcaggatg
    15061 taaccctttg cccacttttt tggcgatctc catcatttta cgtttacgcg cttcaaactg
    15121 tttacggtcg aaatcacgga tgatgtagtg catatcggcc cgttcaacgg tgcctttcat
    15181 gctcgccagg tgatagaaac cttcatagcc ttctgtcatt tccgggcttt catccgccgg
    15241 aacttccgca tgaatacgtg ccgccagcga cagcgcattt accatcactc ctttcgccgt
    15301 gcccggatga acattgttac cgacaatttt gatattgacc gatgcggcgt tgaagttttc
    15361 aaactccagt tcgcctacgc caccaccgtc aacagtgtaa gcccagcggg catcgaaggc
    15421 atcaacatca aaatgtttcg cccctttgcc cacttcttca tccggggtaa aggcgacgcg
    15481 aatatcacca tgcggaatgt ttttctgttg caataccgcc agcgcggtca tgatttctgc
    15541 aatacctgct ttgtcatcgg cacctaacaa ggttttacca tcggtggtaa tcaacgtctg
    15601 acccagtagc tgatgcagca ccgggaacat aaccggtgat aaaacttcat cgccgatacc
    15661 cagcgcaata tcgccaccgc gatagttttc aacaatttgc ggattaacat ttttgccgct
    15721 gcaatccggt gaggtatcca catgagaaat aaagccaatc gccgggatat cgccagggac
    15781 gttagccggt aacgtcgcca tcaaagtgcc cttctcactt aaggtcacat tgataagccc
    15841 catttcttcg agctgctctt tcagcagatg caataacttc cattggcctt ccgtgctggg
    15901 aacctgtctc acccctgctt ttgattgggt atccagagac acgtagttca aaaatcgctc
    15961 aagtagttta tccatgtagt caccctcact ttttgtgaca acattattaa gaagcaagaa
    16021 aagacaaata ttgcgtcagg tcacttttac ccctgcaagc gggaatattt atcagcatta
    16081 ccttaatgaa tataaagcta agccagtaat tcacaacaaa gattggcgat tcaagcggtt
    16141 tgccgtagaa ttaccgccct cattaagagt caccaaggtg gttaaccaca aaccccgcat
    16201 cggtaagcca tccgttgcgt ttacatggga cagagtaaaa aattgaataa acaaccgagt
    16261 tcgctttcac cgctggtgca attggcggga attcgcaaat gctttgatgg taaagaggtc
    16321 attccccagc tggatctgac tatcaacaat ggcgagttcc tcacgctgct tggcccttct
    16381 ggctgcggta aaacaaccgt tcttcgcctg attgcaggtc tggaaactgt tgattccgga
    16441 cgcatcatgc tggataacga ggacatcacc cacgttccgg cggaaaaccg ctatgtgaac
    16501 actgttttcc aaagctatgc acttttcccc cacatgaccg tgttcgaaaa tgtggccttt
    16561 gggttgcgca tgcaaaaaac ccccgctgct gaaattacgc cccgcgtgat ggaagccctg
    16621 cggatggtgc agctggaaac cttcgctcaa cgtaaaccgc atcagctctc tggtggtcaa
    16681 cagcaacgcg tcgccattgc tcgcgcggtg gttaacaagc ctcgtctgtt gttgctggat
    16741 gagtcgctct cagcgctgga ttacaaactg cgtaagcaaa tgcagaacga gctgaaagcg
    16801 ttacagcgta agcttggcat tacattcgtt ttcgtgactc acgaccagga agaagcactc
    16861 accatgtcgg acaggattgt ggtgatgcgc gatggtcgca ttgagcaaga cggcacgccg
    16921 cgtgaaatct acgaagagcc gaaaaacctg tttgttgccg ggttcattgg cgaaattaat
    16981 atgtttaacg ccacggtgat cgagcgactc gacgagcagc gcgtacgcgc caacgttgaa
    17041 ggccgcgaat gtaatatcta cgttaacttc gccgttgaac cggggcaaaa actgcatgtt
    17101 ctgctgcgcc cggaagactt acgtgttgaa gagattaacg acgacaacca cgctgaaggg
    17161 ctgattggtt acgttcgtga gcgtaactac aaaggcatga cgctggagtc ggttgttgaa
    17221 ctggaaaatg gcaagatggt gatggtcagc gaattcttca atgaagacga tcctgacttt
    17281 gaccactctc tcgaccaaaa aatggccatt aattgggtag aaagctggga ggtcgtactg
    17341 gctgatgaag aacacaagta agttccagaa tgtagtgatt gtcactattg tcggttggct
    17401 tgtgttgttt gtctttctgc ccaacctgat gatcattggc accagctttt tgacccgcga
    17461 cgacgccagt ttcgtcaaaa tggtctttac gctggataac tacacgcgtc tgctcgatcc
    17521 gctctatttt gaagtgctat tgcactcgct gaatatggcg ctgatcgcca ccctcgcctg
    17581 cctggtgctg ggctatccat ttgcctggtt tctggcgaag ctgccacaca aggtgcgtcc
    17641 gctgctgctg tttctgctga ttgttccgtt ctggaccaac tcattgattc gtatctacgg
    17701 gctgaaaatt ttcctcagca ccaaaggcta tctcaacgag tttttgctct ggctgggcgt
    17761 tatcgacaca ccaatccgta tcatgttcac gcccagtgcg gtgattatcg gtctggttta
    17821 cattctgctg ccgtttatgg tgatgccgct gtactccagt atcgaaaaac tggataagcc
    17881 gttgcttgag gcggcgcgcg atctcggtgc cagcaagtta cagactttta tccgcatcat
    17941 tattccactg acgatgccgg ggattattgc cggatgtctg ctggtgatgc tgccagcgat
    18001 gggcctgttc tatgtgtccg acctgatggg cggtgcgaaa aacctgctga tcggtaacgt
    18061 catcaaggta cagttcctca atattcgtga ctggccgttt ggtgcagcca ccagcattac
    18121 gctgactatc gtaatgggcc tgatgttgct ggtttactgg cgcgcttctc gtttgctgaa
    18181 taagaaggtg gaactcgaat gatcggtcga ctgcttcgcg gcggttttat gaccgctatc
    18241 tacgcgtacc tgtatatccc aatcattatt ttgattgtga actcctttaa cagctcgcgc
    18301 tttggcatca actggcaggg ttttaccacc aaatggtata gcctgctgat gaacaacgac
    18361 agcctgttac aggcagcgca gcattcacta acaatggcgg tgttttcggc gacgtttgct
    18421 acgcttatcg gttcactgac ggcagttgcg ctgtaccgtt atcgctttcg cggtaagccg
    18481 ttcgttagcg gaatgctgtt tgtggtaatg atgtcaccag atatcgtgat ggcaatttcg
    18541 ctgctggtgc tgtttatgct gctgggtatt cagcttggct tctggtcgct gctgttctcg
    18601 catatcacct tctgcctgcc atttgtggtg gtgacggtgt attcgcgcct gaaaggtttt
    18661 gacgtgcgga tgctggaagc ggcgaaagat ctcggtgcca gcgaatttac cattctgcga
    18721 aaaatcattc tgccactggc aatgccagcg gtggcggcgg gctgggtgtt aagctttacc
    18781 ctgtcgatgg acgatgtggt agtttcttca ttcgtcaccg gaccaagtta tgaaattctg
    18841 ccgttaaaaa tttattcgat ggtcaaagtc ggcgtatcgc cggaagttaa cgcgctggca
    18901 accatattac tggtgctgtc gctggtgatg gttattgcca gccagcttat tgctcgtgat
    18961 aaaacgaaag gtaacacagg ggacgttaaa tgaaaaaatg gtcacgccac ctgctcgcgg
    19021 cgggtgctct ggcactgggc atgagcgccg ctcacgccga tgacaacaac acgctgtatt
    19081 tctacaactg gaccgagtac gtgccgccag gactgcttga acagttcacc aaagaaaccg
    19141 gtattaaggt tatctattcg acttacgagt cgaacgaaac catgtacgcc aagctgaaaa
    19201 cgtacaaaga cggtgcctat gacctggtgg ttccttcaac ctattacgtc gataaaatgc
    19261 gtaaagaggg gatgatccag aagatcgaca agtcgaagtt aacgaacttc agcaatctcg
    19321 atccagacat gctcaacaag ccttttgacc cgaataacga ctactccatt ccgtatatct
    19381 ggggtgcgac ggcgattggt gttaacggtg atgcagtgga tccaaaatct gtcaccagct
    19441 gggccgatct gtggaagcca gagtacaaag gcagccagct gttgaccgac gatgcccgtg
    19501 aagtgttcca gatggcgctg cgtaagctgg gctactccgg taacaccacc gatccgaaag
    19561 agattgaagc tgcatataac gagctgaaaa aactgatgcc aaacgtcgca gcgtttaact
    19621 ccgataaccc ggctaacccg tacatggaag gcgaagttaa cctcggcatg atctggaacg
    19681 gttctgcttt cgttgcacgc caggcgggta cgcctattga cgtggtgtgg ccgaaagaag
    19741 gcggcatatt ctggatggac agcctggcga tcccggcaaa tgccaaaaac aaagaaggcg
    19801 cgctgaaatt gatcaacttc ctgctgcgcc cggatgtggc aaaacaggtt gctgaaacta
    19861 tcggttatcc aacgccaaac cttgcggcgc gtaagctgtt aagtccagaa gtggcaaacg
    19921 ataagacgct ctacccggat gctgaaacca ttaaaaatgg cgaatggcag aatgacgttg
    19981 gcgcagccag cagcatttat gaagagtatt atcagaagct gaaagcagga cgttaattca
    20041 gcaagcgggc ggtggtatta ccgcccgttt tttagttagc aaggatgtgg ctaatgagtc
    20101 aggacagtaa agttttatcc cgctctttcg tcggtattgt ggtccgcttg taggcctgat
    20161 aagcgtagcg catcaggcaa tgcttcctgc tttataatcc ctccagcaac ttttcaacaa
    20221 attctggcac cacctggctt gccgggccgt aatatttctc ggcaaattca ttaccaacct
    20281 gactcggttc aagattcagt tcaacggtgt gagcaccatg tagtttggct tcgtgtacaa
    20341 acccagcagc cggatagaca tgcccggaag taccaatggc aataaagaca tccgccatcg
    20401 acaacgccat ataaatttca tccatgccga gtggcatttc gccaaaccaa acgacgtgcg
    20461 gacgcaaggg tgccgggaac tggcaacaat ggcatttatc ttctggcgta acgtctccgg
    20521 tccagtcgag aacctgacca ctttgcgaac aacgaacttt cagcaattcc ccatgcatat
    20581 gaatgacatt ggtattacct gcgcgttcat gcaggttgtc tatattctgc gttaccagca
    20641 aaaagcgatc gccgagagcg tcttgcagtt tagccagcgc aagatgcgcg gcgttaggct
    20701 gaatttctgg ctgctgcagc tgtcgacgac gggcgttata aaacgcttgc accagttcag
    20761 gatcgcgatc gaaaccttcc ggcgttgcca catcttcaac ccgatgttct tcccacaggc
    20821 catctgcggc gcgaaaggta cgaatacctg attccgcaga aattcctgcc cctgtcagta
    20881 cgagtactct tggtttttcc attgcttccg gcaccacttt atctctgaaa aaaatacgct
    20941 gacgcaaacg ctcgcgcagg cgacgtttat ttttacgaaa acgacttaac cgatgacccc
    21001 gacgcgacag catagcaacc tctgttgttt aatcggttag atgtaggaag gccgcaccac
    21061 gcattcctcc cgcatcaccg tggcgcgcgc gttcaatgcg cggaacacga gctacaggta
    21121 agagatgaca aggcaacctg tccgccagtt gcgttgtgat tgccgggaaa ttcgataagc
    21181 caccaccaat gacgaccagg tcagggtcaa caatggtcag gatatttccc agagaaaccg
    21241 ctaataaatc cagataacgc tcaacgtgcg cccttgcctg ctcatcgcct tgatcataaa
    21301 gcgcaataat ttcgggagcc tgcaacggtt gatgataata gtgttgatac agccacgcaa
    21361 aaccgcgacc agacagataa ttttcaatgc agccatgctg accacagccg cagcggcgta
    21421 acgggaaatc caaccccatc atggttaacg catcaaccgg cagacgcata tggccaaact
    21481 cgccggtaat gtagctttta ccagtaatcg gtttgccgtt gaaaaccagc ccgccgccaa
    21541 cgccggtgcc gagaattaac cccatcacca gtggatattg cgtaaattca tcctcccagg
    21601 cttctgaaag ggcaaaacag ttggcatcgt tatcaaggcg tacatcgcga tcaagacgtg
    21661 cgctcaggtc ggcacgcagc ggtttgccgc tggcagcggg gacattagcg gcatacagct
    21721 taccatcttc tgtttccggc attcccggaa taccgatgcc gacagaacct ttacagccaa
    21781 aacgttggtc agcttcagct accagctcac acactgcatc taaaaatgcg tcatagctgt
    21841 cacgcggtgt cggcacccgc ttttcccact gcaactgccg accgctatca aacacgccaa
    21901 gcgcaatttt tgttccacca atatcaaacc cgtaatacat tgcctctcct tgttttaatg
    21961 tactgccttt actggccgct aaggactcgc gcagggtcaa tattactggc gcgccgcgcc
    22021 ggataccaac ttgccaaaag actcagcaac aatgctgtga ccagtacgta gaagacgtcc
    22081 agccagtgca attccgatgg caggaagtca ataaaataga tatcgctgga gaggaactga
    22141 tgaccgatca gcttttcaat ccactcaata atcggggtaa gttgcagtga aacaactacg
    22201 ccgataacca ccccacacag gctgccgaac agccctgcca gcaatccata ccagacaaag
    22261 atggcgcgaa ttaaaccatc tttcgccccc agcgttctta atactgcgat atcgccactc
    22321 ttgtctttca ccgccatcac taaggtggag acgatgttga aacaggccac gccaatcacc
    22381 agtaccatcg ccagatacat aatggcgcgg atcatctgga tatcgcgata catatagccg
    22441 taagtaccaa tccaactttt aatataaaca tagctgttgg tcacttcacc cgcatcgcgt
    22501 accagcttat tggcgttgaa aacatccgtc attttaaggg caatacctga cacgctggaa
    22561 cccatatcaa gatattgttg ggcatccgcc agcgggatca tggcaaaact gtgatcgagt
    22621 tgaccactca actgcaaaat accggcaatg tgcaaacgca cacgttttgg ctgcatcagt
    22681 ttatgctcgg gattcgagtt ggggatcatg atcgacaccc aatcgccctg cttcactttc
    22741 agcgcatccg ccacgccttt gccgatgata atttgctgtt cgcccgcttt aaaattgcgc
    22801 cacgcatccc cctgaacaaa cgagggtaat gcgctcagac gctgttcctg ttgtgggtta
    22861 acgcccttca cctggattgc gcgcagattc gctccacttt ccaccagacc ggtgaaattg
    22921 atatacggcg cagcggcggc aatacccggc actttctgca cgttatccag tgcttcctgc
    22981 cagttagtcc atggctgatt taccgcttcg atttcgccat gcggcaccac cgccagaatg
    23041 cggttattca gttcgcgttc aaagccgttc atcgcgctta agccgacgat caataccgcc
    23101 acgccgaggg caatgccaat ggtagaaatg acagagatta gcgacaccat gccgccgcgt
    23161 cgccgtccgc gactaaaacg caggccaatc aataacgata aaggcatcgc cattactccg
    23221 cccccatcag gctcagttcc gccgtcagac gtccatcacg catttccagt tggcggctca
    23281 tacgtttcgc cagttgcagg tcgtgagtga ccaccaggaa agcggtgccc tgcaagcgat
    23341 tcaactctcc aagcaactgg aagatgctgt cggcgttacg cgcatcgagg ttaccggtag
    23401 gttcatccgc cagtaccaga cgcggattat tgaccagcgc acgggcaatc gctacacgct
    23461 ggcgttcgcc gccagaaagt tcagatgggc ggtgattcgc acgatgctcc agccccaccg
    23521 cttttaacat ctccagtgca cggctgttga tttcagcggg ctttttcttg ccaatcagca
    23581 gtggcatagc cacgttttcc agggcagtaa aatccggcag caggtggtga aactgataaa
    23641 taaagcccag cttctggttg cgcagttctg ctttcgccgc tgaagacagt ttgctcatcg
    23701 gctgaccgtt aaagatcaca tcgccggagg ttggcgtatc cagcccgcct aacaggtgca
    23761 gcaaggtact tttaccggaa ccagagctac cgacaattgc catcatttca ccttcgccca
    23821 cgctgaaact gacattgtgc agcacatcgg tttgcacact gccttcctga tagcgtttgc
    23881 acaggttgtc gcattgcaac aggatcttat tcataacgta aagcctcagc gggttgtgtg
    23941 gcggcagcgc gccatgaagg gtaaagcgta gacagcagcg cgatagccat cgccaccagc
    24001 gcaataacaa tgacctgtaa aggttcgata gccaccggca gcgccgcgcc atcaagcagg
    24061 acgccgatta tcggcatcag attatttaac tggctggcaa gcagtgcgcc aagcgccgct
    24121 ccgaggattg caccgataat cccggcgctg gccccttgca ccataaagac catcatgatt
    24181 tgtcgcggag ttaagccttg cgtttgcagg atcgcgactt cgccctgctt ctccatcacc
    24241 atcagcccca gcgaggtaat aatgttaaac gccgcaacag cgacaatcag gctcagcagt
    24301 aagcccatca tatttttttc catgcgtacg gcctggaaca gctcgccttt acggtcacgc
    24361 cagtcctgcc atttgctgcc ttcaggcagt ttttgctgac ttaaagagtc aactttcagc
    24421 ggctcatcca gccacaaacg ccagccggta atattggctg ccggataacg catcagacgc
    24481 gaggcatcct caatattcac cagcatttca tagccatcga cttcactgtt ggcggcgaaa
    24541 gtgccaatca cattgaacag gcgctgactt ggaatacgcc ccatcggcgt gaactggctg
    24601 gcagatagca ccatcacgcg gatttgatca ccgcgattga cgcccaactg tgaggcaagt
    24661 tgttcgccga ggatgacatt atatttcccc ggctcgaggt cagtttgttt cacattgacc
    24721 agatacggcg ttagtggatc tttttgcgcc gggtcgatac cgagcatcac cccgaccgcc
    24781 acgctgcgcg cgctttgcag taccacatca ccggtagtaa taggtgcgac gcgattaacg
    24841 ccgtccagtt tgactgccgt ttccgggagt tgctgcgggt taagagagcc atgctcagaa
    24901 gagagaattg cctgtggcat caggccaagg atgttgtttt gcagctcgcg ctcaaagccg
    24961 ttcatcactg acaatactgt gaccagcgcc atcaccccga gggtaatgcc gatggtagaa
    25021 agccaggaga cgaaacgacc gaagcgatcc gctgcacgcc cacgcatgta acgcaagcca
    25081 ataaatagag cgacaggttg gtacatgaaa tccgtctggt tgctgtagca aagacacgag
    25141 tatataagcg aaagcaagat gggtaaacgt ctgaactgta agtgatcgtc agggctaatc
    25201 tgaaaagccc ccgtaaatca atcggctaaa ttgccatgtt ttatcatcaa tcttgccgcc
    25261 tgatcttaat ctgaaaaatc atccaataca gacagatacc cattacacct cgggtcataa
    25321 ccagcaggat caaccgcatg taaattaaaa aagagcgtgt tgttctgatg aaacaaaaag
    25381 agctatggat taaccagatc aaagggttat gtatttgtct ggtggtgatt tatcactcgg
    25441 tcattacctt ttatccgcat atgaccactt ttcagcatcc gttatcggaa gtcctgagca
    25501 aatgctggat ctatttcaat ctttaccttg ccccctttcg tatgccggtt tttttcttta
    25561 tatccggcta tttgattcgc cgctatatcg acagcgtacc gtggggaaat tgtctcgata
    25621 aacggatctg gaacatcttc tgggtgctgg cgctttgggg cgtggtgcag tggctggcac
    25681 ctgagcgcga tttaagcaat gcctccaatg ccgcttatgc cgattccacc ggtgagttcc
    25741 tgcacgggat gatcaccgcc agcaccagct tgtggtatct gtatgcgtta attgtctatt
    25801 tcgtggtatg taaaattttt agccgtctgg cgctgccact attcgccttg tttgtactgc
    25861 tgagtgtggc ggttaatttc gttcccacgc cgtggtgggg aatgaacagt gtgatacgca
    25921 atttgcctta ttacagcctt ggcgcatggt ttggcgcaac aataatgacc tgtgttaaag
    25981 aggtgccgtt gcgccgccat ctgctgatgg cttctttgct taccgttctg gcggtcggtg
    26041 cctggttgtt tactatctcg ctgctgttgt cgctggtatc gactgtggtg atcatgaagc
    26101 tgttttatca gtacgagcaa cgattcggta tgcgctccac cagcctgctg aatgtgattg
    26161 gctccaacac cattgctatc tacaccaccc atcgcattct ggttgaaata ttcagcttaa
    26221 ctctgcttgc gcaaatgaac gcagcacgct ggtcgccgca agtcgaactg acactcctgc
    26281 tggtttatcc ctttgtcagt ttgttcatct gtactgttgc gggcttgctg gtaagaaaac
    26341 tttcacagcg tgcattcagc gatctgttgt tctccccgcc ttcactgccc gctgccgtca
    26401 gttactctcg ttaaaccatc accccagccg gggcgattca atttgctaaa ccatgtcgat
    26461 tacggataat gaacagcatt gcttatcaga gatatgcccc catatgttga ggcatatcct
    26521 aacgagaatc tgacaaccgt tatgcctgaa caatatcgtt atacgctgcc cgtcaaagcg
    26581 ggtgagcagc gtctgctggg cgagttaacc ggcgcagcct gtgcaacgct ggtagcggaa
    26641 attgccgaac gtcacgccgg tccggtggta ctcattgcac cagatatgca aaatgctctg
    26701 cgtttgcatg atgaaatcag ccagttcacc gatcagatgg tgatgaatct ggcggactgg
    26761 gaaactcttc cctacgacag tttttcgcct catcaggaca ttatctcctc gcgcctttcc
    26821 accctttacc agctaccgac gatgcagcgt ggcgtactga ttgttccggt gaatacgctt
    26881 atgcagcgcg tttgcccaca cagttttctc cacggtcatg cgctggtgat gaaaaaaggt
    26941 cagcgcctgt cacgagatgc attacgaacc caactggaca gcgccggtta tcgctatgtt
    27001 gaccaggtga tggagcacgg cgaatacgcc acgcgcggcg cgttgctgga tctcttcccg
    27061 atggggagtg agctgcctta tcgtcttgat ttctttgatg atgaaatcga cagcctgcgg
    27121 gtgtttgacg tcgacagcca gcgcacgctg gaggaagtag aagcgatcaa tctgctgccc
    27181 gcacacgaat ttccgaccga taaagcggca attgaactgt tccgcagcca gtggcgcgat
    27241 accttcgaag tgaagcgcga tccggaacat atttaccagc aagtgagtaa aggcacatta
    27301 cctgccggga tcgagtactg gcagccattg ttcttcagcg aaccactgcc gccgctgttc
    27361 agttatttcc ctgccaatac cttgctcgtg aatactggcg atctggaaac cagtgccgaa
    27421 cgtttccagg ctgacacgct ggcgcgtttt gagaatcgcg gcgtcgatcc gatgcgcccg
    27481 ctgttgccac cacaatcgct ctggctgcgg gtggacgagc tcttctcaga gctgaaaaac
    27541 tggccccgag tgcagctaaa aactgaacat ttaccgacaa aagccgcgaa tgccaattta
    27601 ggtttccaga aactgccaga cctggccgtt caggcgcaac aaaaagcgcc gctggatgcg
    27661 ctgcgtaagt tcctcgagac tttcgacggt ccggtggtgt tctcggtaga aagtgaaggt
    27721 cgccgtgaag cgctgggtga actgctcgca cgaattaaaa ttgctccgca acgcattatg
    27781 cgtcttgatg aagccagcga ccgtgggcgt tatctgatga ttggcgctgc cgaacatggt
    27841 tttgtcgata aggtgcgtaa tctggcgctg atctgcgaaa gcgatctgct cagtgaacgt
    27901 gttgcccgcc gtcgtcagga ttctcgccgc accatcaacc ccgatacact gatccgtaac
    27961 cttgcggagc tgcatattgg tcagccggtg gtccatctgg agcacggcgt cggtcgctat
    28021 gccggaatga ccacgctgga agctggcggc attactggcg agtacctgat gctcacctat
    28081 gccaacgacg ccaaactgta tgttccggtg tcgtcactgc atctgattag ccgttacgca
    28141 ggtggcgcgg aagaaaacgc cccgctgcat aaacttggcg gcgatgcgtg gtcacgcgcg
    28201 cggcagaaag cggcggaaaa agtgcgtgat gtggcggcgg aattgctgga tatctacgcg
    28261 caacgcgccg ccaaagaggg cttcgcgttt aaacacgatc gtgagcagta tcagttgttc
    28321 tgcgacagct tcccgtttga aaccacgccg gatcaggcgc aggccattaa tgcggtactt
    28381 aacgacatgt gtcagccgct ggcaatggat cgtctggtgt gcggcgatgt tggctttggt
    28441 aaaacagaag tggcgatgcg cgccgctttc ctggcagtag ataaccacaa gcaggtagcg
    28501 gtgttggtgc ctaccaccct tcttgcgcag cagcattacg acaacttccg cgaccgtttc
    28561 gccaactggc cggtacgtat cgaaatgatc tcccgtttcc gcagcgccaa agagcagacg
    28621 caaatccttg cggaagtggc ggaagggaaa atcgatattc tgatcggtac gcacaaactg
    28681 ctgcaaagtg acgtcaagtt taaagattta ggcctgctga ttgtcgatga agaacaccgc
    28741 ttcggggtgc gtcataaaga gcgcattaaa gcgatgcgcg cgaacgtgga tattctgacg
    28801 cttactgcaa cgccgatccc acgtacgctg aatatggcaa tgagcggaat gcgtgacctg
    28861 tcgattatcg ccacgccgcc cgcccgtcgt ctggcagtta aaacctttgt ccgtgagtat
    28921 gacagcctgg tggtccggga ggcgatcctg cgtgaaattt tgcgcggagg acaggtttat
    28981 tatctctaca atgatgtgga aaacattcag aaagccgccg aacggctggc agaactggtg
    29041 cctgaagcac ggattgccat cggtcacggg cagatgcgcg agcgcgaact ggaacgggtg
    29101 atgaatgatt tccatcatca acgtttcaac gtgctggttt gtaccaccat tatcgaaacc
    29161 gggatcgaca tcccgacagc caacaccatt atcattgaac gtgcggatca cttcggtctg
    29221 gcgcagctgc accagttacg cggtcgcgtc ggacgttcac atcatcaggc atatgcatgg
    29281 ctgctgacgc cgcatccaaa agcgatgact accgatgcac aaaaacgtct tgaagcgatt
    29341 gcctcgctgg aagatctcgg ggcaggtttt gcgctggcaa cgcacgatct ggagattcgc
    29401 ggcgcgggtg aactgcttgg cgaagaacaa agcggctcaa tggaaaccat cggtttctcg
    29461 ctgtatatgg agttgctgga aaacgccgtc gatgcactga aagccggacg cgagccgtcg
    29521 ctggaagatc tcaccagcca gcaaacagaa gtcgagctgc ggatgccgtc gctattgcca
    29581 gatgatttca tccctgacgt gaacacgcgt ctgtcgttct acaaacgtat tgccagcgcc
    29641 aaaacggaaa acgaactgga agagatcaaa gtcgagctta tcgatcgctt cggcctgctg
    29701 ccagatccgg cgcgtaccct gctggatatt gcccgtctgc gccagcaagc gcagaaactg
    29761 gggatcagga agctggaagg taatgagaaa ggcgggatga tcgaatttgc cgagaagaat
    29821 cacgttaatc cggcctggtt gattggtttg ctgcaaaaac agccgcagca ttaccgcctt
    29881 gatggtccga cgcgcctgaa atttattcag gatttgagtg agcggaaaac gcgtatcgaa
    29941 tgggtacgcc agtttatgcg tgaactggaa gagaacgcga tcgcttaatg taaatcgggg
    30001 gcgtttctgc gcccccatct gcaacattta caaattcttt gcactttcct gcactatccg
    30061 acactgtcac catccataat tcaggcttat ctgtttatta caataacctt atatttatta
    30121 tggatttttg gtgatgatca aaacgcgttt ttctcgctgg ctaacgtttt ttacgttcgc
    30181 cgctgccgtg gcgctggcgc taccggcaaa agccaacacc tggccgctgc cgccagcgag
    30241 cagtcgtctg gttggcgaaa acaaatttca tgtggtggaa aatgacggtg gttctctgga
    30301 agccatcgcc aaaaaatata acgtcggctt tctcgctctg ttacaggcta accccggcgt
    30361 tgatccttac gtaccgcgcg cgggcagcgt gttaacgatc ccgttgccaa ccctacttcc
    30421 agacgcgccg cgcgaaggca ttgtgatcaa cattgcggag ctgcgtctct attactaccc
    30481 gccgggtaaa aattcggtaa ccgtgtatcc aataggtatt ggtcagttag gtggtgacac
    30541 gctgacaccg acaatggtga ccaccgtttc agacaaacgt gcaaacccaa cctggacgcc
    30601 aacggcaaac atccgcgccc gttataaagc acagggaatt gagttgcctg cggtagtgcc
    30661 ggctggaccg gataacccaa tgggccatca tgcgattcgt ctggcggcct atggcggcgt
    30721 ttatttgctt cacggtacga acgccgattt cggcattggc atgcgggtaa gttctggctg
    30781 tattcgtctg cgggatgacg atatcaaaac actctttagc taggtcaccc caggcaccaa
    30841 agtgaatatc atcaacactc cgataaaagt ctctgccgaa ccaaacggtg cgcgtctggt
    30901 tgaagtacat cagccgctgt cagagaagat tgatgacgat ccgcagctgc tgccaattac
    30961 gctgaatagc gcaatgcaat catttaaaga tgcagcacaa actgacgctg aagtgatgca
    31021 acatgtgatg gatgtccgtt ccgggatgcc ggtggatgtc cgccgtcatc aagtgagccc
    31081 acaaactctg taaatcctgg ataaaaaaaa tccccgcgac acggcatgtc gcggggaaaa
    31141 aatcagcagt agcaggcatt aatgagggtt aatgcttatt tataaattac tgctgttcca
    31201 tggagggtat tcggaccggt tacagaagta atacggaaag attttgcgcc catctcatcc
    31261 gctttttgcg ccagctgctc ttccagcgat cccagatttg tccccgcgtt agcactgata
    31321 gtaccgactt tttgttggcc ttctggcgtt gactgaactt cgacagccgc aaagttggca
    31381 aatgacatgg agcttaaaat cgccgcagcg atgagggttt ttacgttttt cataatagtg
    31441 gccttatgca gatgaatgac gtcgcaagaa attacttaac gatcgataac taaatgatag
    31501 atgtgatatg gatcacatac aagatatttt ttataacaat cattaattaa tttaaaaatc
    31561 tttaaatttc agtcaaatag aaagcaatta tttttatggg atctggactg gtgaacggag
    31621 gtgattattt ttataataac ctttcattca cagtcaccag gtacaacggc atcatggcaa
    31681 ctgactcaac acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg
    31741 ccgcgcttga taaggccatg aaattgttct ggcaacacgg ttatgaagcg acttctcttg
    31801 cggacctcgt cgaagcgacc ggagccaaag cgcccacgct atacgcggaa tttaccaaca
    31861 aagaggggtt atttcgtgcc gttctcgacc gctatatcga tcgttttgcc gctaagcatg
    31921 aagcacagct gttttgtgaa gagaaaagcg tggagtctgc gctggctgac tattttgctg
    31981 ccatcgccaa ctgctttacc agcaaagaca ccccggcagg ctgcttcatg atcaacaact
    32041 gcaccaccct ctccccagat tcaggagata tcgccaatac gttgaaatca cgccatgcga
    32101 tgcaagagcg cactttgcag cagtttttat gtcaacgaca agcgcgcggg gaaatcccga
    32161 cccactgtga cgtgacacat ctggcagaat tccttaattg tattattcag gggatgtcga
    32221 tcagcgcacg cgaaggtgca tcgctggaaa aactgatgca gattgccgga acgactttgc
    32281 gtttatggcc cgaactggtg aaataagtcg caaaaaaagc ccctcagcct gagcgaatga
    32341 ggggccaaat tgcagagtac agccttgtta tactttgtta ttcaaaatca gttgcccgtt
    32401 actatctagc gggatctgcg tacccggatc gcggtccatg cggattttgc cctgctgatc
    32461 gccaatctta taggtcacat cataaccgag cattttttct gacttgtcat acaccgtttt
    32521 acaacgctgt tgcgtagtcg tgtaagtatc gctttcctgg agagagccct ggatctggtt
    32581 accggcatat ccaccaccca gcgcccccac aacagtggcg acatctttac cgcgaccacc
    32641 accaaactga tgccctatca cgccaccagc aacagcgccg agcaccgacc cggtaatgcg
    32701 attttcatcc tgcaccggtc gacgatgggt cactgtgacg ttgcgacact cctgacgcgg
    32761 tgttttaacc gtttccttga ttggggttgc agaaacaacc tgagcgtatt gcgggccccg
    32821 ttcaaacacg ttcagactgg ccactgccgc tacgcccaac gcagctgcga caccaatccc
    32881 gatacccgcc aacattgatt tattcacggg aacacctcct tctttgcagt tatgccaatc
    32941 atgcaagcga aggagaaaag cagctatcag acagaagatg aaaagcgcgg gaatgactgg
    33001 aaatacttaa ggattcggag gagtctgaag gaatgcctga tgcgacgctt aacgcgtctt
    33061 atcaggccta ccgctcgtac catttgtcca gggcggataa ggcgtttatg ccacatcagc
    33121 cagtgtacgt cgattaatgc aacttcaaac gcggacggat aacgcggtta atactcccca
    33181 ccagcatcat taatccggtt ttaaagtaac catgcagcgc aatctgatgc attcggtata
    33241 gcgagatata tacaaagcgc gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt
    33301 tacccatcag gctaccaacg gtggagaagt tcgacagcga taccagcgaa ccatgatctt
    33361 tatactgata atttttcagc ggcttaccgt tcatctgcgc cagaatgttg ttcattgcgc
    33421 aagtcgccat ctggtgtgca gcctgagcac gcggcggaac aaagccccct tccggacgcg
    33481 ggcatgacgc gcagtcgcca atagcgtaaa tgtctggatc gcgagtggtt tgcagcgtcg
    33541 gttccaccac cagctggttg atacggttag tttcaagacc accgatatct ttcaggaagt
    33601 ctggcgcttt gatcccggct gcccatacca tcagatcagc ctcaatatat tcgccatctt
    33661 tagtgtgcag gccgccttca tcagcactgg tgaccatggt ttgcgtcagc acgcgaacgc
    33721 caagtttcgt tagctcgttg tgggccgcag cagagatacg tggcggtaat gcaggcaaaa
    33781 tacgttctcc cgcttctacc agcgttacgt tcagggcttc gttggtcagg cctttgtaac
    33841 cgtagctgtg cagttgcttg atcgcgttgt gcaattcagc ggagagttct acacccgtcg
    33901 cgccgccgcc gacaatcgca atgttcactt tgccatttgc gcccaggttg gcagagtatt
    33961 tcaggaacag attcagcatc tcctggtgga agcgacgcgc ctggtgcggg ttatcgagga
    34021 aaatgcagtt ctctttgacg cctggcgtat tgaaatcgtt agaggtgcta cccagcgcca
    34081 tgaccagggt gtcataggcg attttacgtt ccggaaccaa cagttcacct ttctcgtcgc
    34141 gtagttctgc aatagtgatt gttttcgctt cacgatcaat atcaatgacg gaacccaact
    34201 ggaactggaa accatgattg cgcgcatggg ccagatagct caacgcatcg acgccttcat
    34261 caagcgagcc agtcgccact tcgtgcagca gtggtttcca caggtggctg tggttacgat
    34321 cgaccagcgt aattttggct tttttcttac gtcccagctt atgccccagc tgtgttgcca
    34381 tttccagccc accagcaccg ccgccgacaa tcacaatctt tttcaatggc gtagtcaacg
    34441 tgaccccctt aaatttatta accaattgtt aattaatggt tattaacata gctcttaatt
    34501 aacaacaggt tacagcaatg aaaatgttct tcgctattga gaataacatg aatggtgcat
    34561 tggtcatacc aaaattgatg ttaatcaagt tttgttgctt aaaagatagg caactttacc
    34621 gggggagatc aaaaaatagc gtttattatt tgagcaaatt cggaagagta atatagggtg
    34681 aagcgtatat cgagggcgtt tggtcgtcct gataagatgc gcagcatcgc atcaggcatt
    34741 gatgctcgac tgtcggatgc ggcgtggacg ccttatccga cttacagaat tcggagcgca
    34801 tcaggtaccg gcaaggttac ctgatgcgac gagggcattt acccgagggt tttgaacgct
    34861 ttaatgcgct gtaaatgcgg ggagatattc ttgaatttgt gcgtctgctc ttcgtcccag
    34921 acaatctcgt aataatgatg caactcttca gatgtccgct ggctgttaag tgcttcatca
    34981 ttacgcgaca aaataaccag acaacgatcg cgattcttct cgcggaagtt ggtcacacac
    35041 ttagtggcaa tatcggcata ctcttccggg cgatcaatct tgccttccat gttctcataa
    35101 gggaacaaat tagggttgaa gatcacctgg cggatatcgc aaagaaaacc aatccgttcc
    35161 gcccagtatc cgcccaggcc aacgccgcaa attagcggac gctcgtcaac gttcagttgc
    35221 aacattttgt ccacttcttt aagcagatgc tgcatatcat gtttcggatg ccgcgtactg
    35281 tagcttatca agcgtacatc cgggtcaata aactgcaatt gtaagacttt ctcgtggtta
    35341 cccggactgt tagagtcaaa accgtgtaaa tagataatca tcgcttcctc acataagcca
    35401 gggttagtga cctgctttct cttcctgcca gcgttcatgt aactgattca gacgggtgct
    35461 gatcgctttc cagcgagccg agtccatcag ttcctgtcgc gaaaatgaac ctttatgata
    35521 caaacgtgta acacgttctg ccttgatcgg tgacagatta tctaacacgc tgacggcccc
    35581 tttacgatta ttgcagacca ggatcatatc gcaacccgca tccagtgatg cctgcccgcg
    35641 ttcggcataa ctgcccataa tcgcggcacc ttccatcgat aaatcgtcag agaaaatcac
    35701 gccgtcaaaa cccagttcct gacgcaaaac ggttttcagc cagtagggag aaccgctcgc
    35761 cggacgcgga tcaacatcac tgtagatcac atgcgcaggc ataatggcgt cgagtttatt
    35821 ttcgcggatt aaggaactga agaccgacat atctttagcg cgaatctccg cttgtggacg
    35881 tggatcgcac ggtgtttctt tgtgtgagtc tgccgttact gcaccgtgtc ctgggaagtg
    35941 tttcccggtc gttttcattc cggcttcatg cataccatca ataaaccggc tggcaattgc
    36001 cagggctttt tgtggatcgg catgataaga acgctcgcca atcgccgcgc tgatatgccc
    36061 gacatccagc actggcgcaa agctgatatc aatatccata gcgatcattt cgctggccat
    36121 cagccaaccc gcctcttgcg ccagtttgcc gccctcttcc attcccaaca gcgcagcaaa
    36181 ggattgtgct gccggtaagc gggtaaaacc ttcgcgaaaa cgctgcacgc gtccaccttc
    36241 ctgatctacc gccaccacca gatgattgcg cgatgctgcg cggatctggc gcaccagttc
    36301 acgtaactgg gcaggatcat gatagttacg cgtaaagaga atcagccctc ccaccagcgg
    36361 atgcgccagt atttcacgct cttccgcgtc cagttcgtaa ccttcgacat ccaacattac
    36421 tggacccaca ctgctctcct tattgtttta ttaatagctg ccgccaggtg tcatcggcca
    36481 gcctgataaa ttgttgatcg ccggtttgtc gccagcggta ctcaaaccac cctcctttga
    36541 gcatcagcag ccagggaaac catcgcctga cctgacgcca taattgcgcc ggataaatct
    36601 tcgcgcgagt ggcatagtca ttgaccaatt gccggtgctg ttcagtattt tccacccaca
    36661 ccgccgccag ttccagcgcg atatcaccat ctccagcata ctcccagtcg atgagtttta
    36721 accctgatgc gctatgcact aaatttccgg cgtggacatc catatgcaat ggacttaagc
    36781 gtaaaggtcg tggttcccgc gctttgcgca gacgttttaa cattcgcagc caacccacag
    36841 tccgccgcgc cggatcgctt tgctgccagt acagttccag taagggcaac agcgttattc
    36901 gccagccaaa acgtggttgt tgatgtagat aatacagcaa gcctgccagt tcgttggtat
    36961 ccggcaaata cgtttttacc gcgccgggca gatagtcgac taccatccag tcacggagat
    37021 ataaatgcgg cttcggtgca atgcttgcgg gtagttgtga taaagcccga tactggcgca
    37081 agaacgcgga ctgcggcgca tcaggatcgt gcggctgacg cacaacaaaa cgctggccct
    37141 gatgttcaat gagaaaacta ccgccactaa gcccactatt aaacgtcgtg acgggatgaa
    37201 actgcgggaa aaagcgcgac agcaattcgt cgcgcgtgat gggattattg ctgcgaaacg
    37261 gcacctttac ctgaccagat aatttcgccc gtctgcacca gcatcagctg catttgtagg
    37321 gtcggagcgt taacgttgcc agaggcgctg gagtacagca cgtaatgagc gccgacattg
    37381 cgggcaatgc ctatggcttt actacgggtg cctaaactgt cctgcggcga caaacctaac
    37441 tgttgcttag ccatcgacag ttgctgggcg gaaaccaggg taaatttccc gttattagcc
    37501 agcgcatttc gcagcgtttc ggtcgcttct gcggcattca gcgaaccgtt agtacggttg
    37561 ttaacgctat caaccagcag gacgctaccc gcagtcaccc cgtcagcccc aagcatctta
    37621 ctgaccatcg gctgcattgc gccattccag tcataatggc gaatatgcgg cgcaggcggt
    37681 gccgttcgat cttcgtgctc aattgggcct ggctgctgcg ggatcgtcgg caccgagggc
    37741 actgtgggga caggctgttg tggctcggct ggttgttccg gcgctggttt cacttcttct
    37801 accggtgcag gttcacgttg ccccacacac ccggcgagaa acatcgccag cgcggtaatc
    37861 aaggcgtagc gactcatttt tgtcatcaag attcacccct ttacaaatat agataaagtc
    37921 tgactttgtg cgcccccaga aaattggcgc tgccgtacag cgttaccgcc gaatgtgcgg
    37981 gaatggtaac gctgcgtggc ctttccagag gatgcatctc cagccctctg gcgtcatacc
    38041 agtaaaaacg ataatgaacg gtaacgggtt cttgcctttc gttataaagc gttgaggatg
    38101 ctgaaggttg aatatcagac gtcgaaagga cgggcttttc tgcactgatg cccgcagcca
    38161 ataaagatga ctccatcacc agcgattgct catcattcac cggaatttcc ggatgtgaac
    38221 gacagcccac cagcagtaac aacaccagag acaccagccc aaagcatcct tttctcatcg
    38281 ttacagacct ttatgcgcca gcattggtcc cagcggacgg ccacccaaca agtgcatatg
    38341 gatgtggtaa acctcttgtc cgccatggcg gttggtattc atgatcagac gatagccatc
    38401 ttcggcaata ccttcttgct cagcaatttt tgccgctacg gtgatcatgc gtcccagcgc
    38461 ctgctcatgc tcagctgaca cgtcgttcac agtcggaatg aggatattcg gaatgatcag
    38521 aatatgcgtt ggcgcttgcg gcgaaatatc gcgaaacgcc gttaccagat catcctggta
    38581 gacgatatcg gaggggatct cacgacgaat aattttgctg aatatagttt cttctgccac
    38641 gacattttcc tttttcaaat agccatgcgt tgcgccatgc tacgccgcgc cacactgcga
    38701 gtatagagta tggtcgagtt aaacctgctc tttcaacctt aactcacgat ttcttaagca
    38761 aaaaatcgtt ttgctgccgt atatatcgcc attattccca tttctgcctg cgaatgcgct
    38821 gaaaagacat tctctgtttg gtttacacga caaatatgaa tgcgtatatt tctcatttgc
    38881 atttgcattt gcaagcaaaa ttattcgcac actaaatata accgctcatc accctcctcc
    38941 ggcatgagcc tgacgacaac ataaaccaag agatttcaaa tgctttcaac acaatttaac
    39001 agggataatc aatatcaagc catcaccaaa ccgtcactac ttgccggttg catagcactg
    39061 gcactattac cttctgccgc ttttgctgca ccagccactg aagaaacggt gattgttgag
    39121 ggttcagcca cagctccaga tgatggcgaa aatgattaca gcgtaacgtc tacctctgcg
    39181 ggtaccaaaa tgcagatgac tcaacgtgat attcctcagt cggtcactat tgttagccag
    39241 cagcggatgg aagatcagca gttacaaacg ctgggcgaag tgatggaaaa cacgctgggg
    39301 atcagcaaaa gtcaggcgga ttccgatcgt gctctttatt attcccgcgg attccagatc
    39361 gataactata tggttgatgg tatccccacc tattttgaat cgcgctggaa tctgggcgac
    39421 gcactttctg atatggcact gtttgaacgc gtagaagtag tgcgtggcgc gacaggactc
    39481 atgaccggga cgggtaatcc atctgcggca attaatatgg ttcgaaaaca cgcgaccagt
    39541 cgtgaattta aaggcgatgt ctcggcggaa tacggtagct ggaacaaaga acggtatgtg
    39601 gcggatttac aaagcccact caccgaagac ggtaaaatcc gcgcgcgaat tgtcggcggc
    39661 taccagaata acgactcatg gctggaccgc tacaatagtg aaaagacctt cttctcgggc
    39721 attgtcgatg ctgatttagg cgatcttacg acgctttcag ccggttacga atatcagcgc
    39781 attgatgtta atagcccaac ctagggcggt ttaccgcgct ggaatactga tggcagcagc
    39841 aacagttacg atcgcgcaca cagtaccgca cctgactggg cgtacaacga taaagagatc
    39901 aacaaggtct ttatgaccct gaagcagcgg tttgctgata cctggcaagc gacactgaat
    39961 gccacccact ctgaagtcga atttgacagc aaaatgatgt atgtcgatgc ctatgtaaac
    40021 aaagcggatg gtatgctggt tgggccatac agtaattatg gacctggctt tgatcatgtc
    40081 ggcggcaccg gttggaacag tggcaaacgt aaagttgatg cgctggattt gttcgctgac
    40141 ggtagttatg aattgtttgg tcgtcagcac aatctaatgt ttggtggcag ttacagcaaa
    40201 caaaacaatc gttacttcag ttcatgggcc aacatcttcc cggatgaaat tggcagtttc
    40261 tacaacttta atggcaattt cccacaaacc gactggtcac cacagagcct ggcgcaggac
    40321 gataccacac atatgaaatc gttatatgct gccactcgtg tcacccttgc cgatccgctg
    40381 catctgatcc tcggcgcacg ttataccaac tggcgggttg atacgctgac ttacagcatg
    40441 gagaaaaacc acaccacgcc ttacgctggt ctggtgtttg acatcaatga caactggtcg
    40501 acctacgcca gctatacctc tattttccag ccgcaaaatg atcgtgacag ttcaggcaaa
    40561 tatctggctc caatcaccgg taacaactac gagctgggtc tgaaatcgga ctggatgaat
    40621 agccgtctga ccaccacgtt agccatcttc cgtattgagc aggataatgt cgctcagtcc
    40681 accggtacac ctatccccgg cagcaacggc gaaaccgcct ataaagcggt ggatgggaca
    40741 gtcagtaaag gggtggaatt tgaactcaac ggcgcaatta ccgacaactg gcagctgaca
    40801 tttggcgcaa cgcgctatct tgcagaggat aacgaaggaa acgccgttaa tcctaatctg
    40861 ccacgcacca cggttaaaat gttcaccagc tatcggttgc ctgtcatgcc agagttgaca
    40921 gtcggcggtg gtgttaactg gcaaaatcgc gtgtataccg acaccgtgac accgtatggc
    40981 accttccgcg ccgagcaagg tagctacgcg ctggtggatc tcttcacccg ctaccaggtg
    41041 acgaaaaact tctcgttaca ggggaacgtc aataacctgt tcgacaaaac ctacgatacc
    41101 aacatggaag gttctatcgt ctacggcgca ccgcgtaatt tcagcattac cggcacgtat
    41161 caattctgaa taacacctgt aaaaaaggca gccatctggc tgccttagtc tccccaacgt
    41221 cttacggatt agtggttacg gatgtactca tccatctcgg ttttcaggtt atcggattta
    41281 gtaccgaaaa tcgcctgaac accagaacca gcaaccacta cgcccgctgc gcccagtttc
    41341 ttcaggccag cctgatccac tttagacaca tcagcaacgc tgacgcgcag acgggtaata
    41401 catgcgtcga ggttagtaat gttttcttta ccaccaaatg cagcaaccag agccggtgcc
    41461 atttcgctgg tacctgttgc ttttgcatct tcagtcgcgt cttcacgacc cggcgttttc
    41521 agatccagtg ctttaatcag cacgcggaag atggtgtagt aaacaatcgc ataaccgata
    41581 ccgacgatcg ggaacagcca cagtttgctg ctgttaccag acagaacgat gaagtcgatc
    41641 agaccgtgcg agaacgacgt accgtcacgc atccccagaa gaatacagat tgggaatgcc
    41701 aggcctgcca gaatcgcgtg gatgatgtac aggatcggcg caacgaacat gaagaagaac
    41761 tcgatcggct cggtgatacc ggtcaggaac gaggtcagcg ccgcggagat cataataccg
    41821 cccactttcg cgcggttttc tggtttagca gagtgccaga tagcaattgc ggcagctggc
    41881 agaccgtaca ttttgaacag gaagccacca gacagtttac ccgcagtcgg gtcacccgcc
    41941 atataacgcg gaatgtcgcc gtggaaaacc tgacctgctg cgttggtgta ttcaccaatc
    42001 tgcatctgga aaggtacgtt ccagatgtgg tgcagaccaa acggtaccag acaacgttcg
    42061 atgaaaccgt aaatgccaaa cgcaactacc gggttctggt aagcagccca ctgagagaag
    42121 gtctggattg cagaaccaat cggcggccaa atgaaggaca gcacaacgcc agtaaagatg
    42181 gcagccaggc cagaaatgat cggcacaaag cgtttaccgg caaagaagcc aagatactca
    42241 ggcagcttaa tacggtagaa acggttaaac atgtacgctg caatcgcacc ggagataatc
    42301 cctccgagta cgccagtatc cgccaagtgt ttagaggcga tttcttcagc aggtaaatgc
    42361 agtaccagtg gcgcaaccac ggccatggtt ttaaccatga tgccataggc aacaactgcg
    42421 gccagcgcgg atacgccatc gttattggta aagccgaggg cgacaccgat cgcaaaaatc
    42481 agtggcatgt ttgcaaagac ggaaccgcct gcttctgcca taacatgcga tacaacggcg
    42541 ggcagccagc tgaaattcgc ggaaccgacg cccagcagaa tacctgcgat aggtagtacg
    42601 gataccggca gcatcagcga tttaccgacc ttttgcaggt tagcaaatgc attcttaaac
    42661 ataattgaga gtgctcctga gtataggtgc tttttttacg ttctcacgcg tggcaagggg
    42721 ggagagcctc gccgtgtaca gggcatctaa gcgcccttta tttattacac agagtaaaat
    42781 aattcagtgc caatttgttt gacggctatc acgtttcaac ttcagatcat tggagaagtt
    42841 gcacattttg acaaaacata tgtcacaata tttcaaacgc ggttattcac cgcccaattt
    42901 tcggcggtga actttactcg ttttagccat taattacgag ctttaaaaaa actcattcaa
    42961 cggatggatt gaaggcggga agcgtcgatg tgaaacagac gggcgaagtt atcggtggtt
    43021 acctgcgcca gttcttcaac ggcaacacct ttcaacacag ccatgtattc tgcaacgtca
    43081 cgaaccatcg caggttgatt ctcttttcct cgatgcggta ccggcgcaag gtaaggtgag
    43141 tcagtttcca ccagtaatcg atccaggggg acataacgcg cagcatcgca cagttgctcc
    43201 gcattacgga aggtcacaat gccggaaaag gagatgtaaa atccgagatc cagtaattta
    43261 cccgccgttt ctctgtcctc tgtaaaacag tgtagtacgc caccgcaatc cgtcactttt
    43321 tcttcgcgca gaattgccag cgtatcggca cgggcgtcac gggtatggac gataaccggc
    43381 ttgttcagtt cacgaccaat ctggatatga tggatgaagg actcttgctg acgtacttta
    43441 gtttccggtg tgtaataata atccagcccg gtttcaccca gcgctacaac accctcttct
    43501 gccgccagac ggcgtaaatc ttctacatcg tagggatcat tctggttgag tggatgcacg
    43561 ccacaagaaa atacgacgtt gtcacgttcg cctaccagat cccgcatatg taaataaccc
    43621 ggtaatgttg tggcgactgc cagacaaaat ttcacatcgc gtgcggcggc tttcgccaga
    43681 acgtcatcca cgtccttatg caaagattca taatccagac catcgagatg gcagtgtgag
    43741 tcgactaaaa acatgatgtc tctcttaaag atgaggaacc ggtagcacaa cgcccggttg
    43801 caggtaatgc tcaatacgca gtaaaagatc ggtgatgaga agctcgcggt tgatgcctgt
    43861 gacagacatt aactgttcac gaatgtggca aacatccccc agtatagcct gcaggcgcga
    43921 gggagaaaga tggtttgcca gttcggcgac caggcccggc acatcaacat tggtcacctg
    43981 cgcagcgcca tgatggcgtt ttagcgcatc catcagcaac gttgccaccc agtgtaaacg
    44041 cgccggagct tgttcatgat taagggccgc cagcagcgaa taccagtcgc ccgatggcac
    44101 gctatatgcc aacgcctgac acaatgtttc acgagcctgc cagttatctc cctgaaacaa
    44161 cgccagtgcc gcgccaggcg aaccggcgct taagcgcaat gcggcaagta atgcatcctg
    44221 tgacattgtc acttcgcgtg aaagccaagt cacggcgtac tgttctggag gcggcgcaag
    44281 gtaatgtaac cgacaacgac tacgtaatgt tgccagtaaa cgttcaggct cgcgggtagc
    44341 caggaaaaac caggtttctg ctggtggctc ttcaagcgtt ttcagcaatg cgttagccgc
    44401 ggcgtcggtc agtaaggcag catcggttac ccagacaact ttcgcaccac ctaggcgtgc
    44461 gtgctcattc agcttttcgg tgacctcacg taccgcatca atgcccagcg tatttttccc
    44521 tttttcggga gccagggtgt agtaatcggg atgcgtgcca gcctgcatca actgacatcc
    44581 acgacagtga ccgcaacttt tgtggccctg cggttgttgg cagagtaaat aacggctcag
    44641 ggcgtagatt aaagcatcat cgcccatgcc cggtaacgcc tgaatgagta gcgcatggtg
    44701 acctcttccg gcctgatagc tggctaccag tttttcgaaa tcaggtcgta accatggata
    44761 ccatctcatg cgtccaactc cttcacccag tgggtcacgg tagtgcggat tgcatccatc
    44821 acggcctcca gcggctgggt ggcatcaatg gtatgaatac ttttatcttg tgcagccagt
    44881 tccagatagc gggcgcgggt gcgattaaag aaatcgaaag actcttgctc aatacgatcc
    44941 agctcgccgc gcgcacgcgc gcgttttaag ccaacttccg gggtaacatc gagatagagc
    45001 gttaagtcgg ggcgaaaatc cccgagaaca gcatcacgca gtgttgccag catatgttgg
    45061 tcaataccac gtcctccgcc ctgatacgcc tgagtggaga gatcgtggcg atcgccaatc
    45121 acccaggtgc cgttagccag cgcaggtttg atgaccgttt ctaccagttg aacgcgcgcg
    45181 gcataaaaca tcagaacttc ggctttatcg gtaatgactt catcgcctac cgatttgata
    45241 tccagcacca ggcttcttaa cttttcggca agttgcgtac cgccaggttc ccgagtgaaa
    45301 accatgtcgc ggatacccag ttgctcgagc gtctcaacca ccacattacg cgcggtagtt
    45361 ttgcctgcgc cttccagccc ctcaatgacg atatacttac tgcgcatttt tttccttaag
    45421 cactttcaga taatcctgca cagacttgtt atgactggca agattggtat taaacgtgtg
    45481 accaccttta ccatcggcca caaaatagag atacggcgtt tttgccggat gcgcagcagc
    45541 cttcagcgaa tccgcccccg gcgtagctat cgcacccggc ggcaaaccgg taatggtata
    45601 ggtgttatac gctgtcggcg tttccaggtc tgcacgagaa agtttgccat tataacgctc
    45661 tcccatcccg taaatcacgg ttgggtcggt ctgcaggcgc ataccaatgc gtaaacggtt
    45721 gataaatacc gaggcaacct gatcgcgttc actggcaacg gcggtttctt tttcgataat
    45781 tgatgccatc gtcaccagct gatttttatc tttataaggc agaccgtccg cacgcccttc
    45841 ccaggcgcta tcgaccgctt tcaccatttt cttgtgagct cgcttgagta acgcgacatc
    45901 ggtggtattg gcggtataca tcaaggtgtc tggccagaac caaccttcaa tccactccgg
    45961 gttttccagt tcaagtgcct gcgctacggt ggcgtactta tcgtcactca gcgtgtgctt
    46021 gatatacgga gcctcacgca attgcttgag gtagtcgctc agacgcatcc cttctaccag
    46081 tcgcagcggg aactgtgctt ctttaccgct ttccaacaat ttcagcatct cgcgcacggt
    46141 catctgcggt gtaaagcggt aagtcccggc tttaaagtga gaaagatccg gttcgatacg
    46201 cagcagccat tgaaaaaccc gtgggcgatt gatgatctta tcagcataaa gctgttcacc
    46261 gagcgccaga cgtccggtcc ctggcttcag ggtaaatatc gtctcttctt tgataagcaa
    46321 tttgctgtcg gcaagatggc gaaccttcca gacgcccaca ccagcggcga tacccagtac
    46381 caccagcaat aacaagataa tcaataacac ttttttcatg actaattcgg gcgctcacaa
    46441 agtggggcta aatattcata taacgttgct gacgaaaagg tgacatcgcc acaggcacgt
    46501 acgggcatca ctggcattaa cgcattacaa ataaccatct catctgcctg caacgcctcc
    46561 tccagcgagg cttgcacttc gacaagctga taagaggatt gtgccagtaa acggatacag
    46621 aattgtcgca taatgccgtt aacacctgcc tgatccagtc gcggcgtata aactacattg
    46681 cccttccgcc agaacaaatt agccgcacag cattccgtaa cccacccttc gctgtcaagg
    46741 accagcgcct catcagcgtt tgtctgctca agatgagagc gaatcaatac ttgctcaagc
    46801 cgattaagat gtttaatacc tgcaagatga ggattgcgcc ccagccgcac cgggcttagc
    46861 gccaacgtca tcccttcgtt acgcaaacgg tcgtaatgtg caggataagc cgtaacggag
    46921 agaatccgcg ttgctggtcc gctgttcaat gtgctgtacc ctcgcccgcc actaccgcga
    46981 ctgatcacga ctttcagcac accattttgc tgttctgctg ccagcgtttt catctcttgt
    47041 tcaagctgag gccagaagtc acaggaaatc atcaaccgct gacaagcatc ctgtagtcgc
    47101 tggatatgcg ccgataacaa actgacttta ccgtcgataa ctctggcggt ggtaaaacaa
    47161 ccatcaccaa actgcgttgc ccgatcgctt actgccagcg attcctgctt ataaccgtta
    47221 attaagaaca taccggctcc ttatcagaga attgttagtg tggcagcatg ttcactacgg
    47281 aacaagtcgg aataaaagct aagaaaaaag gcccgcaagc ggacctttta tatgggtgga
    47341 aaatgacaac ttagatcttt ttaaagatca aagaaccatt agtgccaccg aagccgaagg
    47401 agttacacag cgtgtattcc attccgctga cctgacgtgc ttcatgcggt acgaaatcca
    47461 gatcgcaacc ttcatccggg ttatccaggt tgatggttgg cggaacagcc tgatcgcgca
    47521 gcgccaggat ggagtagata gattctactg cacccgccgc acctaacagg tgaccggtca
    47581 tagatttcgt ggagcttacc aacacacggc ttgcagcttc accgaagatg gttttcaccg
    47641 cctgcgcttc agctttatcg ccagccggcg tagaagtacc gtgcgcgtta acgtagccaa
    47701 tctgactcgc ttcaatgcct gcatcacgca gagcatttgc cattgccaga gctgcgcctg
    47761 cgccattttc tggcggtgac gtcatatgat aagcatcgct gctcatacca aagccgacga
    47821 gttcagcgta aattttcgca ccgcgttttt tcgcgtgttc gtactcttca agtaccagca
    47881 taccggcacc atcgcccagt acgaagccat cacgctcttt atcccacggg cggctcgccg
    47941 cttgcgggtt atcattgcgg gtagataatg cacgtgccgc gccaaaacca ccaacgccca
    48001 gcggcgtact ggctttctct gcgccacctg caaccatcac gtcagcatcg ccatacgcga
    48061 taatacgcgc agcatggcca atgttgtgca cgccggaagt acaggcggtc gcgatagaga
    48121 tgcttgggcc acgcaggcca tacatgatag tcagatgacc tgccaccatg ttcacaatcg
    48181 ttgacggaac gaagaatggg ctgatcttac gtggaccacc gttcatcaga gatgtgtggt
    48241 tttcttcgat cagtccgagg ccgccaatcc cggagccaat tgcggcacca atgcgggttg
    48301 cgttctcttc cgttatttca aggccagaat cctgcatggc ctgaacgcca gcgacaattc
    48361 catattgaat gaaggcatcc atcttgcgct gttctttgcg cgagataatg tcctcacagc
    48421 taaaatcctt tactaagcca gcaaatttcg ttgcataggc gctagtatcg aaatggtcga
    48481 ttaggctgat gccactctga ccggcaagca gagctttcca ggtagactct acggtattgc
    48541 cgacaggaga caacatgccc agtccggtca caactacacg acgcttagac acgtttgtcc
    48601 tccaaggagg gaaaaatgat tctagtggga caaaaagata aaactcaggc ggtcgaacga
    48661 ccgcctggag atgttcactt acgcctggtg gccgttgatg taatcaatgg cagcctgaac
    48721 ggtggtgatt ttctcagctt cttcgtccgg aatctcagta tcaaactctt cttccagagc
    48781 cattaccagc tcaacggtgt caagagaatc cgcgcccagg tcttcaacga aagaagcatt
    48841 gttggtaact tcttcctgct taacgcccag ctgttcgccg ataattttct taacgcgttc
    48901 ttcgatagtg ctcatactct taaatttcct atcaaaactc gctttcgcga tggttttcgt
    48961 agtgtataaa atgttgaaaa atttgcaact aaatcccggc aggtcttacc acgattttac
    49021 gttattttga ggccttaccc cccaataacg caaatcattt tgcactaatt attactcata
    49081 accacgcaga ctgcgcgcaa catgagcatt ttgtgcaaat cgcggtcaga ccatgtacat
    49141 cccgccgttc acatgcaaag tttcacccgt gatgtaagct gcttcgtcgg atgccaggaa
    49201 tgcaaccgcg ttggcgattt cctgtgcgcc gccgaggcga cccgcaggaa cctgcgccag
    49261 gatacccgca cgctggtcat cgctcagcgc acgtgtcatg tccgtttcaa taaagcccgg
    49321 agcaacaacg tttacagtaa taccgcgtga cgcaacttcg cgcgccagtg atttactgaa
    49381 gccgatcaag cccgctttcg ccgcagcgta gttggcctga ccgccatttc ccatggtacc
    49441 aaccacagaa ccgatagtga taatacgacc atgacgcttt ttcatcatag cgcgcattac
    49501 cgcttttgac agacggaaaa cagatgaaag gttagtttcg ataatatcgt tccactcttc
    49561 atctttcatt cgcattaaca ggttatcacg agtgataccg gcattattga ccaggatatc
    49621 cacttcacca aattctgcgc gaattttttc cagaacagat tcgatagatg ccgggtcggt
    49681 cacattcaac atcagacctt tgccgttggc acctaaataa tcactgatcg cctgagcgcc
    49741 attttcactg gtcgcagtgc caataacttt cgcgccacgg gctgcgagcg tttcagcaat
    49801 tgcgcggcca attccgcggc ttgcaccggt taccagtgcg atttttcctt caaaattcat
    49861 gattttcctc ttttaaagct cgagcgccgc tgccatcgct gaaggttcgt tcagcgccga
    49921 ggcggtcagg gtgtcgacaa tgcgtttcgt caggccagta agcaccttgc ccgggccgac
    49981 ttcatagaga tgttctacgc cttgcgctgc catgtactca acagacttcg tccactgaac
    50041 cgggttatac aactgacgta ccagtgcgtc acggatggca tcaccattgg tttcgcattt
    50101 cacatcaacg ttattcacaa cagaaactgt tggtgcgtta aaggtgattt tcgctaattc
    50161 tactgccagt ttgtcggctg ctggtttcat cagcgcacag tgagacggta cgctcactgg
    50221 taacggcagc gcgcgttttg cgccagcagc tttacaggca gcgccagcac gctcaaccgc
    50281 ttctttatga ccggcaataa ccacctgtcc cggagagtta aagtttaccg gagaaacgac
    50341 ctgaccttct gcagcttctt cacacgcttt cgcaatagac gcatcatcca gaccgatgat
    50401 tgccgccata gcgcccgtgc cttccggtac ggcttcttgc atgaacttac cgcgcatctc
    50461 aaccaaacgc accgcatcag cgaaatcaat cacgccagcg caaaccagcg cggagtattc
    50521 ccccaggctg tgaccggcca tcattgccgg tgctttaccg ccctgctgct gccatacgcg
    50581 atacagcgca acagatgcag tcaacagcgc tggctgggtt tgccaggttt tattcagttc
    50641 ttcagctggc ccctgctggg tcagcgccca caggtcgtag cccagcgccg cagaagcttc
    50701 agcaaacgtt tcttcgacaa ttggatagct cgccgccata tcagccagca ttccaacggt
    50761 ttgagaaccc tgtccaggga acacaaatgc aaattgcgtc atgttttaat ccttatccta
    50821 gaaacgaacc agcgcggagc cccaagtgaa tccaccgcca aaggcttcaa gcagaaccaa
    50881 ctgccccggc ttaatgcgcc cgtcgcgtac agcttcatcc agcgcgcacg ggacagaggc
    50941 cgcagaggta ttaccgtggc gatccagcgt caccacgaca ttgtccatcg acataccgag
    51001 ttttttcgcc gttgcactga taatacgcag gttagcctga tgcggaacca gccagtccag
    51061 ttgagaacgg ccaagattat tcgccgccag cgtctcatca acgatgtgcg ccagttccgt
    51121 taccgcaacc ttgaagactt cgttgcccgc catcgtcaga tgaattgaat tctctggatt
    51181 cacgcggtcg gcgtttggca gcgtcagcaa ttcaccataa ctaccgtcgg catgcagatg
    51241 ggtggaaatg attcccggct cttcagaggc agccagcacc gcagcgcccg cgccatcgcc
    51301 aaaaataata atagtcccac gatctgttgg atcgcaggtg cgcgccagta catcggaacc
    51361 gacgaccaga gcatacttca ccgccccaga tttcacgtat tgatcggcta cgcttaatgc
    51421 ataggtgaaa cctgcgcagg ctgctgcaac gtcaaatgcc gggcaacctt taatgcccaa
    51481 catgctttga atctgacaag ctgcgctcgg gaaagcgtgc gtagcagaag tcgttgccac
    51541 aacgatcagg ccaatctggt ctttctcaat gcccgccatc tcaattgcgc gtgtcgccgc
    51601 ttcaaagccc atggttgaaa cggtttcgtt tggcgcggca atgtggcgtt cgcggatacc
    51661 ggtacgagtg acaatccact cgtcagaggt gtccaccatt ttttccaaat cggcgtttgt
    51721 ccgcacttgt tcgggcagat agctgccagt accaataatc ttcgtataca tgtacgctca
    51781 gtcacttttc ggttatatac cgtcacttgc aaactgcgag ttcgctggca gcgtcctgct
    51841 accgcagagt tccgcttttg ccaccgtcca gcagctcaaa accagctggg tatacagatt
    51901 ccaggcgagc ggcaattcgc tgaggaactt gtcgctgcac cgcctgcact gcctgttcaa
    51961 tcgcgaccgc aaaagctcgc tgattggctg caccatgact ttttatcacc gtgccgcgca
    52021 atcctaacag acaggcgccg ttatactggt cggggttgag gtgactgaat cgcctcgtca
    52081 ggctcttttg tagccaacgc tttaataaca gtagccacca cgaccgtttt ttcccttcac
    52141 cctgagattt cagcagagaa aggaacatcc tgacaacacc ttccatcgtc tttaacgtga
    52201 catttcctgt aaagccgtca caaaccagca catctgtctt gccagttaac aactcattgg
    52261 cttcaagata gccgatataa ttgatagaag ggattgtttt aagcaccgct gaggcatccc
    52321 gaatactgtc aagacccttt acttcttctt caccaatatt gagcaacgcc acgcgaggat
    52381 tgggaatttc caccacctct tcagccagaa ctgagcccat aatggcaaat tgcaccaaca
    52441 ttgtgctgtc acaatcgacg ttggccccta aatcgaggac caccgttttg cccttttgct
    52501 gatgtggtaa taccgtcacc agcgccggac gctcaatccc ctccaggggc ttgagtaata
    52561 attttgccag ccccatcagt gccccggtat taccggcact gacacaggct tgcgctcgac
    52621 cttctttcac cagctccagg gccatacgca ttgaactccc acgactggcg cggatagctt
    52681 gcgaaggccg ggcatcactg gcgataactg actgcgcagg aataatctgc agacgcgaac
    52741 gttgttcaaa gtcagcttta gcaagtaatg gcgtgatggc gtcgggattg ccgactaaaa
    52801 gaagagtgag ttgcgaatta gaattcagtg cctgcaatgc tgcaggcact gtcacggaag
    52861 ggccaaaatc ccctcccatg acatctaacg ccagggttag acgtgtcaag gtatcgtcgc
    52921 cgcccggttt ggtctttccc cagttgcctg gggaaaatcc tcactaagct tcatcacgca
    52981 gacgcgtgat tacttagcga tgaccttgcg gccgcggtag taaccgtcgg cagtgatgtg
    53041 gtgacgcagg tgtttttcac cagaagtttt gtctacagac aggctggtga ctgcggtcag
    53101 cgcgtcatgg gaacgacgca tgccacgttt ggaacgggtt ggtttattct gttgtacggc
    53161 catggacctt actcctcaat tacggtttat ccccgatcca acggggagca ccaattactt
    53221 acgctttaag ctggctaata cggcaaatgg gtttggcttt tgcacttctt caggcagttc
    53281 accaaagacc atgtccgctt cggacacttc acagtgttca gaatcatgca ccggaactac
    53341 cggcaaggcg aggatgattt catcttcaac cattgcaagc agatcgattt caccgaattc
    53401 gttaacctca atcggttcat acgcttccgg cagtgcttca gcctgttcgt ctgaacgcac
    53461 aggactaaaa caatacgttg tgtagacctg atgagtaaac ggcttcccgc aacgctgaca
    53521 ctcgagcgtt accgtcacct tcgcatcgcc gtttaacact gcgagacgtt ggttatcgat
    53581 agcgaacgac atggagcatt ccacatcact gtccacactg actacggatt cggcgacgcg
    53641 ctcaacctga tcaggggtat agataccctg gtaatcaagg cgtttttgag ccgtacgaac
    53701 cggatcgaga gtcaggggga attttacctt ttgcataggg cgcgcatatt aactttgtaa
    53761 cgtcatagag tcaaagaaaa aggcagcctg tggttgcctt ttgccaataa ttcgcacaca
    53821 ttgcgggtta ctgctttatt ttcgttcagt gctgataacg aacatcaacc tgcaaaacga
    53881 agcgcatagt ttaaaatggc tatcatcaat acgctatatc tgatggaaaa aaatatgcct
    53941 aaacttattt tagcctccac ctcgccatgg cgtcgcgctc ttctggaaaa actgcaaatc
    54001 tcttttgaat gtgcagcacc tgaggtcgac gaaaccccac gcagcgacga atcaccgcga
    54061 cagttggtgc ttcgactggc acaagaaaaa gcgcaatctc tggcgtcacg ttatccggat
    54121 catttaatta ttggttcaga ccaggtatgt gttcttgatg gcgaaatcac cggtaaaccg
    54181 ttaacggaag aaaatgcccg tctgcaatta cgcaaagcca gcggcaatat cgtcaccttc
    54241 tatactggac tggcgttgtt taattcggcg aatgggcatc tacaaacaga agtggagcct
    54301 tttgacgtcc atttccgtca cctaagcgag gcggagattg ataattacgt gcgtaaggag
    54361 catcccctgc actgcgcggg tagctttaag agtgaaggat ttggcattac gctgtttgag
    54421 cgtttagagg gacgtgaccc taacacgctg gttggtttac cgctaatcgc gctgtgtcag
    54481 atgttacgtc gggaagggaa aaacccgctg atgggatagt tgtttgcgcc ggatgcggtt
    54541 acgtcgcatc cggcaatcgg tgcaggcggc ctgatgcggc gtaaacgcct tatcaggcca
    54601 accgcccatt atcaggctta tatgcttgta ttagcgcgcg ttacgcagtt tttgcaaaca
    54661 acgcttcaaa ccttcatcca tcggcgcttc gatacgcatc acctcaccgg tccccggatg
    54721 ggtaaacttc aacgctgcgg cgtgcaggaa cagacgattt aatcccgtgc ctgcttcagt
    54781 gagctgtctg tcaaattcac ggtcaccgta gcgatcgtca aaggcaatcg gatgacccgc
    54841 atactgtgta tgcacacgga tctgatgagt acgtccggtt accgggctac aacgcaccag
    54901 ggtggcaaat gcatagcgtt cttccacttt aaagcgtgtt tccgacggtt tgccttcctg
    54961 actgacacgc acgatacgtt cgccgctttg cagaatattt ttcaataacg gcgcttgaac
    55021 gctcttcaca tgcgactgcc actgaccgcg caccagcgcc aggtaatctt tttgcattcc
    55081 tttttcacgt aattgctcat gcagagaacg caacgccgag cgttttttcg ctaccagcaa
    55141 cacacctgaa gtgtcccggt cgagacgatg aaccagttca aggaaccgcg cttccgggcg
    55201 caacgcccgc aaaccttcga taacgccgaa gcttaaacca ctgccgccat gtaccgccgt
    55261 accggaaggt ttattcagca ccaggatatg atcatcttca tataagatga cgtccgccag
    55321 cgccgccacc ttttgcagat gtggcgaaac cgcctcttct tcccgctcag caacgcgaac
    55381 cggtgggata cgcacctcat cacccgcttc gagtttatat tcaggcttaa tacgtttttt
    55441 gttcacccgc acttcacctt tacgcaaaat acggtaaatc atactttttg gtacgccttt
    55501 caattgggta cgcaaaaagt tatcgatacg ttgccccgct tcgtcagcgg tgatagcaac
    55561 aatttttacg gatggagtct ctgttttcat ggtgggcgat tctaattagc caacaggatt
    55621 cgcgccactc atttttctat gcttatattt agttttgcac cttattactt cactacgtga
    55681 tcactttatt gatggttatt aaaccaatca ccagcaagaa gtgaaaaaac tgtgagtaag
    55741 cgggtgataa atggtaaaag tcatcttgct ataacaaggc ttgcagtgga ataatgaggc
    55801 cgtttccgtg tccatccttg ttaaaacaag aaattttacg gaataaccca ttttgcccga
    55861 ccgatcatcc acgcagcaat ggcgtaagac gtattgatct ttcaggcagt tagcgggctg
    55921 cgggttgcag tccttaccgg tagatggaaa tatttctgga gagtaatacc cagtctgttt
    55981 ctttgataat tgcgctgttt ttccgcatga aaaacgggca accgacactc tgcgcctctt
    56041 tgagctgacg ataaccgtga ggttggcgac gcgactagac acgaggccat cggttcacac
    56101 ccggaaaggc gttactttgc ccgcagctta gtcgtcaatg taagaataat gagtaagtta
    56161 cgatgaaaag aatgttaatc aacgcaactc agcaggaaga gttgcgcgtt gcccttgtag
    56221 atgggcagcg tctgtatgac ctggatatcg aaagtccagg gcacgagcag aaaaaggcaa
    56281 acatctacaa aggtaaaatc acccgcattg aaccgagtct ggaagctgct tttgttgatt
    56341 acggcgctga acgtcacggt ttcctcccac taaaagaaat tgcccgcgaa tatttccctg
    56401 ctaactacag tgctcatggt cgtcccaaca ttaaagatgt gttgcgtgaa ggtcaggaag
    56461 tcattgttca gatcgataaa gaagagcgcg gcaacaaagg cgcggcatta accaccttta
    56521 tcagtctggc gggtagctat ctggttctga tgccaaataa cccgcgcgca ggtggcattt
    56581 ctcgccgtat cgaaggcgat gaccgtaccg aattaaaaga agcactggca agccttgaac
    56641 tgccggaagg catggggctt atcgtgcgca ccgctggcgt aggcaaatct gctgaggcgc
    56701 tgcaatggga tttaagcttc cgcctgaaac actgggaagc catcaaaaaa gccgctgaaa
    56761 gccgcccggc tccgttcctg attcatcagg agagcaacgt aatcgttcgc gccttccgcg
    56821 attatttacg tcaggatatc ggcgaaatcc ttatcgataa cccgaaagtc ctcgaactgg
    56881 cacgtcagca tatcgctgca ttaggtcgcc cggatttcag cagcaaaatc aaactgtaca
    56941 ccggcgagat cccgctgttc agccactacc agatcgagtc gcagatcgag tccgccttcc
    57001 agcgtgaagt ccgtctgcca tccggtggtt ccattgttat cgacagcacc gaagcgttaa
    57061 cagccatcga catcaactcc gcacgcgcga cccgcggcgg cgatatcgaa gaaactgcgt
    57121 ttaacaccaa cctcgaagcc gccgatgaga ttgctcgtca gctgcgcctg cgtgacctcg
    57181 gcggcctgat tgttatcgac ttcatcgaca tgacgccagt acgccaccag cgtgcggtag
    57241 aaaaccgtct gcgtgaagcg gtgcgtcagg accgtgcgcg tattcaaatc agccatattt
    57301 ctcgctttgg cctgctggaa atgtcccgcc agcgcctgag cccatcactg ggtgaatcca
    57361 gccatcacgt ctgcccgcgc tgctccggta ccggtaccgt gcgtgacaac gaatcgctgt
    57421 cgctctctat tctgcgtttg atcgaagaag aagcgctgaa agagaacacc caggaagttc
    57481 acgccattgt tcctgtgcca atcgcttctt atctgctgaa tgaaaaacgt tctgcggtga
    57541 atgcgattga aacgcgtcag gacggtgttc gctgcgtgat tgtgccaaac gatcagatgg
    57601 aaaccccgca ctaccacgtg ttgcgcgtgc gcaaagggga agaaacgccg accttaagct
    57661 acatgctgcc gaagctgcat gaagaagcga tggcgctgcc gtctgaagaa gagttcgctg
    57721 aacgtaagcg tcctgaacaa cctgcactgg caacctttgc catgccggat gtgccgccag
    57781 cgccaacccc agctgaacct gccgcgactg tcgtagcccc agcaccgaaa gctgcaacgg
    57841 caacaccagc agctcctgca caacctgggc tgttaagccg cttcttcggc gcactgaaag
    57901 cgctgttcag cggtggtgaa gaagccaaac cgaccgagca accaacaccg aaagcagaag
    57961 cgaaaccgga acgtcaacag gatcgtcgca aacctcgtca gagcaaccgc cgtgaccgta
    58021 atgagcgccg cgacacccgt agtgaacgta ctgaaggcaa cgataatcgc gaagaaaacc
    58081 gtcgtaatcg tcgccaggca cagcagcaaa ctgccgagac gcgtgagagc cgtcagcagg
    58141 ctgaggtaac ggaaaaagca cgtaccaccg acgagcagca agcgccgcgt cgtgaacgta
    58201 gccgccgccg taacgatgat aaacgtcagg cgcaacaaga agcgaaggcg ctgaatgttg
    58261 aagagcaatc tgttcaggaa accgaacagg aagaacgtgt acgtccggtt cagccgcgtc
    58321 gtaaacagcg tcagctcaat cagaaagtgc gttacgagca aagcgtagcc gaagaagcgg
    58381 tagtcgcacc ggtggttgaa gaaactgccg ctgccgaacc aattgttcag gaagctccag
    58441 ctccacgcac agaactggtg aaagtcccgc tgccagtcgt agcgcaaact gcaccagaac
    58501 agcaagaaga gaacaatgct gataaccgtg acaacggtgg catgccgcgt cgttctcgcc
    58561 gctcgcctcg tcacctgcgc gtgagtggtc agcgtcgtcg tcgctatcgt gacgagcgtt
    58621 atccaaccca gtcgccaatg ccgttgaccg tagcgtgcgc gtctccggaa ctggcctctg
    58681 gcaaagtctg gatccgctat ccaattgtac gtccgcaaga tgtacaagtt gaagagcagc
    58741 gcgaacagga agaagtacaa gtgcagccga tggtgactga ggtccctgtc gccgccgctg
    58801 tcgaacctgt tgttagcgcg ccagttgttg aagaaatggc tgaagtcgta gaagcccccg
    58861 taccggttgc cgaaccgcaa ccggaagtgg ttgaaacgac gcatcctgaa gtgattgccg
    58921 ccgcggtaac tgaacagccg caggtgatta ccgagtctga tgttgccgta gcccaggaag
    58981 tcgcagaaca cgcagaaccg gtggttgaac cgcaggaaga gacggcagat attgaagaag
    59041 ttgccgaaac tgctgaggtt gtggttgctg aacctgaagt tgttgctcaa cctgccgcgc
    59101 cagtcgtcgc tgaagtcgca gcagaagttg aaacggtagc cgcggttgaa ccagagatca
    59161 ccgttgagca taaccacgct accgcgccaa tgacgcgcgc tccggcaccg gaatatgttc
    59221 cggaggcacc gcgtcacagt gactggcagc gccctacttt tgccttcgaa ggtaaaggtg
    59281 ccgcaggtgg tcatacggca acgcatcatg cctctgccgc tcctgcgcgt ccgcaacctg
    59341 ttgagtaata attagctcaa agtaatcaag ccctggtaac tgccagggct tttttatttc
    59401 atgtttgaat caacatatta aaattcatca aatggcatcc ttgctaacca acaatgcaaa
    59461 ataggcaaaa aaaatgcccg ccccggcgat cccagagcgg gcagacaaaa catacccagt
    59521 ttcatgatat gttttaaagc gagcttattt gttgagctgg aagagcgaca atccctgcat
    59581 atcggtaaat gctttatacg atgcctgcaa tgccgtttgc tgcatgatgt aagatgaaat
    59641 agttgcattc cagtccacat caaccagatc gctcatctgc tgcgtttgcc ctaaagcgcg
    59701 atcgctacct aaggaatcca gcgactccag tttgttcagc tgcgtgccta attccgtgcg
    59761 aacggtaagg acattattca gcgagttttt cagtccacgg ttggttttat ccaatgccgc
    59821 agcggcgatt tctttatccg cttcgctatc cgcgaccggc gttttcaggg ctgcgatggc
    59881 gctatccagc atggcaaaaa gattggtttc agaagcgcta ccgtctggtt ccgctaccgc
    59941 gttactggta atactgtcga aaattttgtc gcccgtatgt cctatcacca tcgaacgcga
    60001 agcatcgacc tgttgcttaa tactttctgt tccgccgaca taatcaccgt tagcctcact
    60061 aaacggcgca gtctctgttt tataaccggc aaaaatgtag cgcccattac cgtcagtggt
    60121 gttcgccaga ttcagcaact ggtcacgaag cccctgaata tccgtagcca gcgaggcccg
    60181 gtcatcgtca ctcaaggtgc cattgctggc gtagacaatt ttttcctgag cattctggat
    60241 agcagtggtg acctggctaa gtacactctc ttccagtgac actttttgag tggcgaaagt
    60301 acgtgccagc gtgtactggc tgttttgcgc ctgtgcctgg gagagaacaa cagcttgtga
    60361 tgcagcaatg ggatcgtctg aagggttaac gactcgctta cccgtcgaca tctgctcgcc
    60421 gtacttcatc cattctgcct gagaattggt gatgccgcgc atgttttgct ggtacatcat
    60481 ctgggtactg aaacgcattt tatctcccct tagcgaatgt taatcagcgc atcaaaaatc
    60541 gcgtttgccg tctgcagaac ctgcgcattc gccaggtaat actgctgaaa acgttgcaga
    60601 tttccgtact cctcatcgag attgacaccg gaaatcgact gctgctgatt ggaaagctgc
    60661 gtcaccacat taccttgcgt ggtgctactg gttttcaacg tcgcggtttt attaccgata
    60721 tcactcacta acgaggcata agcgtcgtta aaggatttcg caccgcccac cgttttactg
    60781 ttgctttgca gatccagcag ggcctgaccg ttgcggttgt cgctatcacc cgcatcttct
    60841 tcgctcgcca tcgcgatttt cgcttcgtcg gtgattaata catccatgtt gacgatggcg
    60901 tcacttactg gtttcagcgt gaagctgtcg ttaacggcag gcgttcctgt aaacgtcaac
    60961 tccagaccat caaatgccac tttaccgttg gcatctggtg tcgccgtaaa agtggtattg
    61021 ctggcaaggc gggtgacctg ccactgatta ttatcgaacg agattttgta atctgtcgcc
    61081 agtaccacgg aggcatcagt taccgtggca ccgatcgcaa cgtcaccttt gtttttcgtg
    61141 ttttgctgaa ccgcgggctt accgatagta aagaaatctt caccagcatc gccgttagca
    61201 tcaaatccgg ctttgtgttg gctgttgaaa gcctcggcaa atgccagcgc cagttgtcca
    61261 agcgtattac gcgtctggtc cagatcctga gaacggaatg tcagaatgcc gcccagcgac
    61321 ccggtattca gtaatttctc cgggatctca atattgcctg ccgtcccatc aacgtaagcg
    61381 acagtcgtac gagaagggtc ggcgctggaa ggaactgccg ccagttgccg cgccgtactt
    61441 ccctgaacca gtgagtaacc attggcatcg tgatgttata agtaccgcca tcctgaacgc
    61501 tgacttctac gccaacaatc tggtttaatt cgctcaccag ttgatcgcgt tgatccagca
    61561 gattgttagg tgacgcccct gcccccacgc ctgtcaggcg cgagatttga tcgttcaggc
    61621 tggcaatttg tttagcgtaa ttgttgatct gatcaacgct ggcacctatc gcgatattga
    61681 cctgtttgtc ctggtcgcgc agatattgat cggtggtttt aaactgattc accaaccctt
    61741 ctgatttccc aatcagcgcc tggcgcgccg ccgggtcttc cgcgttactc accagcgttt
    61801 gcaggctggt gaagaaatcc tgcatctgtg ttgccagcga agaggtactg gtggagagca
    61861 tattgtcgat tttcgacatc tgctcatagc gggcggtcag gccgctactt tgcgtctgca
    61921 cagcacgtaa ctggttggta ataaacgtat catactcacg ctgcacacca gaaacgtaga
    61981 cgccattgcc aacccagccg ccagcgccca acgtgctatt ggcctgcgcc ataatagtgg
    62041 tttggcgggt atatccggca acgttatagc tggagatatt attacttgcc gtatttaacg
    62101 ccgcctgggc cgcgttcagt ccgctcatgg cgttattgat caagctggac atggaggttc
    62161 cttataagcc ttcaattaca gagtattatc ggcagcgacc cgccggactt gagttattca
    62221 gaacagatta tcaatgttca tgctgtaggt tttgctcacc tggtcgctta tcgatttcat
    62281 ctgctggatc atgttggtga gtttacgggc atagtgagga tcggtagcat agcccgcgtc
    62341 ctgtaggacc tgcgccccct gttccgcact cgcggcggtc gtcacggcgg cgtagcgcgg
    62401 gttacgtgtt aacagcccaa cgtaatccga caatgcttcc agatacgagc tgtagacccg
    62461 aaacttcgct tttactttct tcgcttcgcc gttttcatat tcagtcgtgg tgatttcagt
    62521 gacttgccct ttccagttgc cagaggcttt gacaccaaac aggttatagc ttggctcgcc
    62581 gttttcgcgg cggatttgcc gttgccccca accagattca agcgcagcct gagcgaggat
    62641 caaatgatgt ggcacaccgc tttgctggct tgccagttgg gcgggcagcg agagttgcgc
    62701 gaggaatgct ctactgtcac ccggcagcga atcatcgtag ttacgtggta cggccttttg
    62761 caccagctgc gaaagcgtct ggttttgata acgcaccacg gtttcgagcg ggaatttcat
    62821 cggtgctgct ggcgtggact cctctggcaa tggttgttct ggcgtcatct gtttaaccat
    62881 catctctgca agccccagac ctttgcccgc cgtcatctgt tgggcaattt gctggtcata
    62941 catactggta tacagacgag tgtgctcgct gctgaacagg ccatcttttg gtaaagcgtc
    63001 gcgcatgctt ttcaacatca tctgcacgaa catcccttcc acctgacggg ccaccggacg
    63061 gatatttgcc gccggatctt cgctcgcttt cgcctttagt tcgttgagtg attgcgcatc
    63121 ccaggccgca cttgccagta gtttgctgtc gctgatcatc agatgatttc cagttttgcc
    63181 cgcagacatc ccgcactttg cattgattgc aaaatagaca tcagatccat cggcgtagcg
    63241 cccagcgcat tgagcgcacg caccacgtta ttgaggctgg cgctggaacg tacgctttgc
    63301 agcgaaccgc cgctctggcg taaatcgatc tgcgtttgtg gagtaacaac agtctgtcca
    63361 ccaccaaacg gtgtatctgg ctggctgaca ttggcctgac gattaactgt tactgagaga
    63421 tttccctgcg ctaccgcgca gctgtcgagg gtcacttctc gattcatcac caccgaaccg
    63481 gtgcgcgagt taatcactac tttagcgtcc tgcggggtga cattaacctg catattctgg
    63541 atatcggcaa ggaaacggac ctgggaactg ttgccacttg gtacgcgcac ctgaatagtc
    63601 cgcgcatcta acgcggtggc gctgccatat ccacgcacgc ggttgatggt gtcagcgatt
    63661 tgctgcgcca tgctgaaatc ttcgtcgtta agttgcaaat taagggtatt cccgacgcca
    63721 aactggctgg gcaattcacg ttcaataacc gcaccattgg tgatccgtcc accgttcagt
    63781 tggttaacct gaacactgct accgcccgcg gaggctcctg cgccgccaac cagaatattg
    63841 ccctgcgcca gcgcatacac ctgactgtca acgcccttaa gcggtgtcat caacaacgta
    63901 cctccacgca agcttttggc attacccatg gaagaaacca ccacatcgat ggtttgcccc
    63961 tgacgtccaa acggaggaag tgacgctgtc accattacca cagcgacgtt ttttagctgc
    64021 atattggtgc ccgtcggaac ggtaattccc agctgtgaga gcatgttatt aagcgtttgt
    64081 gtggtaaacg gcgtctgggt tgtctggtca ccagtgccat ccagccccac taccaggcca
    64141 tagccaatca gtgagttttg ccttaccccc tgaacactgg tgagatcgcg aatacgctca
    64201 gcctgagccg ccgtcgtaac cagtagaaga attaatgcag agagaaattt aatcaccaca
    64261 gcctcactta cattggcgac aggttaagga agaaacgctg caaccagccc atattttgcg
    64321 cttcgttaat gtagccattg cctacgtatt caatacgcgc atccgccacc tgagtagacg
    64381 gtacggtatt gctgccgctg atagtgcgtg gattaaccac gcccgagaag cgaataaatt
    64441 cggtaccctg attaatggca atctgttttt cacccaccac atgcaggttg ccgttgacca
    64501 gtacctggtc aaccgtcacc gtcaacgtgc cgctaaaggt attgctggca ttggccccgc
    64561 cctttccgtt gaacgtgtta ccaccggagg cttcgacatc ggcacgagcg ttaccaaaca
    64621 gcccctgcaa atagcgcggc acagtatcaa agccaaaatt agttttaccg tcacggctgg
    64681 cattcgcaga ggagcttttg ctggcgctga cgttctcctg caacacgatg gtcagcgtat
    64741 cgccaatatt gcgtggtcga cgatcttcaa acagcggttg atagccatag ttaatcggct
    64801 gagcagactg gaaaatagaa ccgttggcga cgggcgtcgg accgggaacc ggttgtgcac
    64861 tggtcgcccc ctgcaccagc ggcgtggagg gtatccaggc gcagccggtt agtgaaagca
    64921 ccaacaagct ggaaatggca taagtatgcg cagcgttttt ttgcatggct atcatcttca
    64981 aaaatcagta aaccggtgaa cctgccaccg gttatgcctt agagttgcgt cagtttttgc
    65041 agcatctgat cggtggtgga caccgcttta ctgttgattt cgtaagcgcg ttgcacctga
    65101 atcatattga ccagttcttc cgccacgttg acgttagacg tttcaacata cccttgatac
    65161 agcagtcccg cgccgttcag gcccggcgtg ctttcgttcg gtgcaccgga ggattgcgtt
    65221 tcggtgtaga ggttttcgcc aatgctctcc agtccggtat cattcataaa ggtggtgaga
    65281 ttgagctgcc caacctgaac cggagctgcc tggccttgtt gggttacgct gaccacgcca
    65341 tcacgaccga tggtgatact taacgcattc gccggaatgg tgatcgctgg ctgcacctga
    65401 aaaccaccag ccgtcaccag ctgcccgttc tgatccacct ggaaagagcc gtcacgggta
    65461 taggctgatg agccatctgg caacattacc tggaaaaagc cctgcccttt aatcgcgaca
    65521 tctttgctgt tattggtctg cgacagattg ccctggctgt gtaagcgttc agtggcgacc
    65581 gggcgtaccc ccgtgccgat ttgtaatccg gagggtaagg tggtttgttc ggaagactgt
    65641 gcccccggct ggcgaatggt ttgataaagc agatcttcaa acaccgcgcg ctgacgctta
    65701 aaaccgttag tactgacgtt tgccaggttg ttggcaatga cgtccatatt ggtttgctgg
    65761 gcgtcaaggc ccgttttggc gatccataat gaactgatca tataatgtcc tgtagtggat
    65821 tgctgacaag cagagcagaa gcacgcgaca gtgtgaattt cccctcaccc taaccctctc
    65881 cccaaagggg cgagggaata gtccgcgcgc ttttattgca atgtcatcaa tcgttcaatt
    65941 aactcatcga cagcagttgg ttggcacggc ctgcgttatc atcgacgctg ctgatcacct
    66001 tcatctgcat ttcaaaacgc cgcgcgctgg caatcatgtc gctcattgcc gcaacggcat
    66061 tgacgttact gccttccaga acccccgaca tcacacgcaa ggttggatct gcctgcagta
    66121 ccggccctcg cgtggcctgg gtttctgcgc ttaaacgaaa aatgccgtcg tcaccgcgct
    66181 gcacttcgct gcccgtggct ttcaccagtt tcagacgccc tactggcgca actgtatttg
    66241 ccggatcgcc cggattgagc gccgagattg tgccatcggc agcaatcgtg atttccgccc
    66301 cttccggcac agcaattggc cccgcctcgc ctatcaccgg atgcccctga attgtcagtt
    66361 gcccggtggg atcaacctga atgctgccat tacgcgtata cccttcgctg ccgtcagcgg
    66421 tctgcacggc cagccatcct gctgcaacgc aacgtccagc gggcgcgagg tgtaatccat
    66481 cttgccgggc gtcatgtctg cacccggtgt tgacgccgtt accagcgtgc gcgtgggcag
    66541 tgaaagccct tccaccggca ctgcgcgcaa ggcgtttaac tgcgcgcgaa aaccgggcgt
    66601 agaggcattg gccagattac tggcggttac cgcctgttga ttcagcgtct ggctggctgc
    66661 tcccatcgca gtataaattg cgtgatccat tgagctatcc cgtcagcgat tagcgtaagt
    66721 taacccgcgt gttgaggatc tggtcctggg ttttgatggt ctgggcgtta gactgatagt
    66781 tacgctgggc aacgatcata ttgaccagtt ctttactgag atcgacgttg gacgcttcca
    66841 gcgcaccgtt ggtcagggtg ccaaagtttc ccgtcccggc tgtccccaac agcgccacgc
    66901 cagaagattg cgtcgcagac cagacgttgt cgccttcgga tgccagacct tcgttgttgg
    66961 caaagttcgc cagtacaatc tgccccagca gttgggtttg ttcgttggaa tagttgccga
    67021 caaccgtacc gtcatcattg atttgataac tcaccagatc gcccggtttg tagccattct
    67081 gggtggttgc cacaatgttg ttagcgccgg tattttgctg catggagttg aggaagctca
    67141 gactaaacgt ggcgggttct gcaccgttaa ttgcgccggt tgcgatatta ttcgccatcg
    67201 caccatccac taatgtgcca ttagcattaa attccagtgt tgtcgctgtc ttcgcaatgc
    67261 tgtttggatc actgctatcc tgggtgtaga cctgccagtt attatccccg gtcttcacaa
    67321 aatagacgct catgtcatga gcattaccct gactgtcgaa aacagtcacc gaaccttttt
    67381 tgttatagct atccgcattg ctggcgctga atggcgtaac agtaggaagc ggatcactgg
    67441 aattcaggtt gatctgcatc gacgccgtgg tggtagtttt cgctgccatc agggtattcg
    67501 ggatcgaaat attggtcgga ttcgcccctt gctgaatagt cggcggcgta ccggttgccg
    67561 ggtaacccgt cagctgtaaa ccttgcatat tcaacaggtt acggttttca tccagcttaa
    67621 attgtccgtt acggctgtag aacaccgaac cgttgctgtc taccagacgg aaaaaaccgt
    67681 tctggctgat agcaacgtcc agacctcgcc cggtgttggt ggtcgtgcca tcggtaaagt
    67741 cctgagtgat accggcaact tttaccccca gtcccacttt cgaaccggca aacatatcgg
    67801 caaaagaggc cgtgcctgat ttaaagccgt aggtggcgga gttggcgata ttgttgccaa
    67861 taacatcgag gttggtggcg gcagcgttta atccgctaac cgcttgagaa aaggccatga
    67921 ctgactcctg aaatgtgaag gctcaaatta tctgccgtac ttcgtcgagg gtggtggtgc
    67981 cgtaagtgcc gagatccagc gtattaccgc tgttgccgcg gatcacaccc tgcaccagag
    68041 caaactgcag cggctgggca accagttgtg taccaccgtt actggcgcta atcgctacat
    68101 tgtaagaacc gttcggcgca gtgctgccat cagtcaacgt accgtcccag gtgaaactgt
    68161 gaactccggc ggtcagttca ccaatatcaa tggtgcgcac aaccgcgcca tttttatcgg
    68221 tgatggtggc cgtaactttg tctgccgcct gttgcagctc aacaccaaac ggcgtggtcg
    68281 tggtcacagc cccttcttca ctgccggttc ccgcaagaac agttgtgccg gggatcatca
    68341 cgccgtgacc gatcaggtta ctggcctgta acgactggct gttatcaatc tgtccggaaa
    68401 tagatccgag cgtggtattg agtttttcaa tcccgctgac cgtgctgatt tgtgccaatt
    68461 gcgacgtcag ctcgttgttt tccattggat tggtcgggtc ctggtttttc agctgcgcca
    68521 ccagcaaagt cagaaaactg ctttgtaaat ctgcggcgtt gctgcccgtg agcgaactac
    68581 tgctggtggt actgacgccg gtatttgtcg gatcggtggt ggttaccgca atggacatag
    68641 ctttctcctt tattgaccga gcgtaagggt tttcagcatc atgcttttca ccgtgttgag
    68701 cacttcaaca ttggcctgat agctgcgtga cgccgacatg gtgttaacca tctccccgac
    68761 gacatcaacg ttcggcattt ttacgtagcc ctttgcatct gccagcggat tacctggttc
    68821 ataaaccagt ttgtccgggg cctgactttc ttaacatcgg caacctttac gccgcctgtc
    68881 gcagcacctg gtgcagcgtt aacctggaat accacctgtt ttgcccgata tggctgtcca
    68941 tcaggaccgg tcacgctatc agcatttgcc agattactgg ccgccacgtt caggcgctgg
    69001 gactgggcag ttaacgccga cccggcgata tcaaaaatat tcagcagtgc catccgttaa
    69061 tttccgctct gtaaaacgtt catcatgcct ttgatttgcc cgctcaacgc gctaaggctc
    69121 atctggtatt gcaggctgtt atcggcaaac tgggtgactt cctgacgctg ggcgcgcaga
    69181 ttgagcgcct cttgttgaaa acgtaaggcg gcgtcgagct tatcgagcat atctcctccg
    69241 caggtatcaa aattctgcca tcagcttaaa tgcctttacg cgcgcgttat cggcggaata
    69301 aacgcaaaat gggtcgctat ttatgccgtt gatggtcatt gcggacaggt acaactcacg
    69361 ttgtagaaat ggctgggggc gaaaatgctg acaataaaac gtagcgtggc gatcatcgcg
    69421 atactgttca gtccgttaag tacggcgagc aatctcacat cgcaattgca caactttttt
    69481 agcgcccaac tcgcgggggt aagtgatgag gttcgtgttt ctattcgtac agcgcccaat
    69541 ctactaccgc catgcgagca gccattgctt tcgatgagca ataattcccg cctgtggggc
    69601 aatgtgaatg tgttggcacg ctgcggtaac gacaaacgat atttacaggt taatgtgcag
    69661 gccacaggaa attatgtggt tgccgcgatg cctattgtgc ggggaggaaa gctggaagct
    69721 ggcaatgtca aactgaaacg tggacgcctg gataccctgc caccgcgtac ggtgctggat
    69781 atcaatcaac ttgttgatgc cgttagcctg cgcgatctat cacccgatca acctatccag
    69841 ttaacccagt ttcgccaggc atggcgggta aaagcgggac aacgcgtcaa tgtgatcgcc
    69901 agcggtgatg ggtttagcgc caacgcagaa ggtcaggcgc tgaacaatgc agccgtcgca
    69961 cagaatgcgc gggtgcgcat ggtatcggga caggtagtca gcggcgttgt tgatgcagat
    70021 gggaatattc ttataaacct gtaagctgtt aaagattacc cgtcccttgc cgataaataa
    70081 gcaacacatg ataaaagcgc cctcaatgag gaataaacca tgagtattga tcgcacttcg
    70141 cctctgaagc ctgtaagcac cgttcaaccg cgcgaaacca ctgacgcgcc ggtaacgaac
    70201 agccgggcgg caaaaacaac cgcctccacc agcaccagtg tgacgttaag cgacgcgcaa
    70261 gcaaaactga tgcaacccgg cagcagtgat atcaatcttg aacgtgtcga agcgttaaaa
    70321 ctggcgattc gtaacggtga actaaaaatg gacaccggca aaattgccga tgcgctgatc
    70381 aacgaagcgc agcaagactt gcagagtaac tgatcgtatg acacgtcttg cagagatcct
    70441 cgaccagatg tccgctgtgc ttaacgatct caaaacggta atggatcatg agcagcaaca
    70501 tctctctatg gggcagatca actgcagcca gttgcaatgg attacagaac aaaaaagctc
    70561 actgctggcg acgctggatt acctcgaaca gttacgcaag aaagaaccca atacagcaaa
    70621 tagcgttgat attagtcaac gctggcagga aattactgtg aaaacgcagc aactacgcca
    70681 aatgaatcaa cataacggct ggttactgga aggacagatt gagcgcaatc aacaggcgct
    70741 ggaaatgttg aaaccgcatc aggaaccgac gctatatggg gcgaacggtc agacctcaac
    70801 accccatcgc ggcagtaaaa agatttcgat ctgaatatta tctccggcct gcactgcagg
    70861 ccggaatgca ttgttacacc gtccggcggg caaattcttt aactttgaag cccagtaccg
    70921 ccagtgcagc gaagtacgcg gcaatccccg ccagcacgac cgccattaaa cgcagtaagc
    70981 gccagggcat ggtacccaat gaccactccg gcatgatatg taacatacct aaaagcacgc
    71041 cagacatcac cagtaccgcc accaccagac gcaacagaaa cgccatccag ccgggttgcg
    71101 gagtaaagat tttctgctta cgcaactgcc agtaaagcag cgaagcattc agacacgccg
    71161 ccagaccaat agaaagtgac agcccggcat gtttcaacgg accaataaac gccaggttca
    71221 tcaattgcgt taaaatcagc gtaacgatgg caattttcac tggcgtttta atgtcttggc
    71281 gggaataaaa gccaggagcc aacactttca ctacaatcag gccaatcaaa cccaccgagt
    71341 aggcaattaa cgcccgctgg gtcatcagcg catcaaacgc ggtaaattta ccgtactgga
    71401 acagcgaaac ggttaacgga cctgaaagaa tgcccaacgc aaccgcactc ggcagtgcca
    71461 acaggaaaca aagacgcaac ccccagtcca tcaaacggtt gtattcatca tgattgccac
    71521 tggcaaaact tttcgacagc gacggcagca aaatggtgcc aagcgccacg cccagcacac
    71581 cggagggaaa ctccattaag cggtcggcgt aatacatcca agacaccgaa ccggaagcaa
    71641 gaaacgaggc aaaaatggtg ttgatgatta aggagatctg gctcacagag acgccaagga
    71701 tcgccggtcc catctgtttc accacgcgca ttgctccagc atcgtggaag ttaatgcgcg
    71761 gcaggaccag catgccgatc ttcttcaggt gcggtagctg atacaccagc tgcaggatgc
    71821 cgccgaccgt aacagcccac gccagcgcca gcaccggtgg gttaaagtac ggtgcggcaa
    71881 acagcgcgaa accaatcatg ctgatgttaa gcagtgttgg cgcaaacgcc ggaatcgaga
    71941 agcggttcca cgtattcaga atcgctccca ccagcgacgc cagggagatc agcaagatat
    72001 agggaaaggt aatctttagt agctggctgg tcagggcaaa tttgtcagct gtgtcagcga
    72061 agcctggcgc ggtcaccatg atcacccacg gtgcggcgag catgccagcg accgtcacaa
    72121 ccgccagcgc aagtgtcagc aggccagaaa cataagagac aaagacccgc gtggcgtctt
    72181 caccctgctt acttttatat tccgccagaa tcggtacaaa tgcctgggaa aatgcccctt
    72241 cggcaaagat acggcgtaac aagttaggaa gtttaaaagc gacgaaaaag gcgtcggttg
    72301 ccatccctgc gccaaagatt ctggcgacaa ttgcgtctcg tgcgaagcca agcacacgcg
    72361 aaaacatggt catcgagctg acggcggcca gcgattttaa taaattcatc ggtgttctaa
    72421 tccggaccca cgggcaaaac gcctgcaagg caggcgctga aagaggcgaa tagtctacct
    72481 agattatggt gaattgctac cgccagatgt tacagggttt attcactcat cgcatcgcgc
    72541 cagatcttgt caacgatacg ttgcgccagc actgcctatt cgccggcggt ttgcggaact
    72601 gtctgatttt gcacgcattc aatgaagtgc cgcgcacagc cgacaaatcc acgttgctca
    72661 agagtgctct gccaaccagg aatcggttta ttcactacac cctgcccgcg ctcctcacgc
    72721 cattcgcgca tatccgtaat gtcgatgagc gcaccgtcag tcacggcctg cacggtttca
    72781 cgctgacttc cggcacggcg atgcatacaa gtggtgatct gcaaaggacc ggccgaaaaa
    72841 tggtgctcgg caaacagcat ttcgccagcg tcgttagtca gtagcgtacc gccatccaga
    72901 gaggctttgc cgcccgacaa ccacagcgcg gtatccacca catgcagata atcatccagc
    72961 aacgtgaaat aaagatcgtg tggcccgacg ctattgctac gatgtttatc cattcttagc
    73021 gaggctgcgg tggcgagttg cgtttttaac tcaccgtaga gtggtgcgaa acgacggtta
    73081 aaaccgacca tcaacgtcag ttttttccgc gccgccagtt ccaccagcct ttcagcatcg
    73141 cgcagatttt ctgccagcgg tttatcgaca cagacatgta cccccgcatt gagtaacgta
    73201 ctgaccacgt caaagtggct ggcggtgctg gagtgcacaa aaaccgcatc acaactggcg
    73261 gcaaggctgg ataacgaatc ggcataagga atgcgccagc tgtcacaaat tggcagggct
    73321 ttggcgcgcg taggcgacta ggctccttgt aacgtccagt cagacgctgc cgccagcacc
    73381 ggtaaccacg ctttttgcgc aatgccgcct aatcccacta cgccgatacg taattttttc
    73441 actttaatct cccagatggg ccagcaacga atcaagacgt tgtttcagtt ctgccacttc
    73501 gatttccagg gcttcgacgc gggtctgtaa atcaccgtca accatgttcg acatagccgt
    73561 taccgccggc tgatcttcaa cctcaccact gaaaagatgc atgtagcggc tttcgcgttt
    73621 acccggttcg cgggccagac gcaccacaaa aggaccatct tcgcgattcg ccagttgttc
    73681 cagcgtcgac tccacttccg ccatatcgct gaattcatac attcgcgcgg cgcggctgcg
    73741 cagttcaccc agcgtctggg caccacgcaa taacaacgtg gtgattaacg ccacttctgc
    73801 ggcgctcagt ttcagatcgc caaattctga attacaaaaa cgttgctcat atttggtgac
    73861 ccgattacca aaaccgctca ctgtgcgtag ataatgacgt ttgaccagat tatccagctg
    73921 ttcctgcact tcggattcac tcagattcat gaccggttca cggttcgttt tctgattaca
    73981 ggccgtgact acaccattga ctgagagcag gtattgctcc ggcgtggtca cctgcttttc
    74041 cagcaagcaa ccaattacgc gagcttccag ggcagtaagt tgatatttca tgagattctc
    74101 cttagcggcc gggcgtccag tctggggtag ttaatgccgt cagtacgtga tcgcgccatt
    74161 gtccatcaat caacagatag tctttcgcat agccttcttt ttcaaaaccc agtcgcgcca
    74221 gtaaatcacc gctgcgttta ttgtgcggca tataattagc cataatgcga tgaatatgtt
    74281 gggtgcgctg catataacga atggctgcgg tcagggcttc aaacatgagt cctttgccct
    74341 gccatttttg cccaatcgaa taaccgagat agcaggtatg aaaagagccg cgaacaacat
    74401 tggaaaaatt ggcaacgcca ataatctctt tttcgtccgg gtcgaataag ccaaagtaga
    74461 aagctgaacc ttgtttatga aattcgttaa tcatccctag cctggcctgc cagcctgatg
    74521 gataacagtg gctttcgtcg cgcactggct cccagggctt gaggaaatgg cgattctctg
    74581 cgtaataatc cgccagacgc caggcatcac gatcatgcac cagacgcacg accagtcggt
    74641 ctgtggttaa gcgcactttt ggcacgttac tgcgatagcc aaacattcta tacctactcc
    74701 ttcccgtaac gtctttaata cgctgtaagt attactatac ctgcgcaacc agaccctgtg
    74761 aaaagtccaa aatgccattt tctgcattat tcacatattt gataaaaact ctctatcaaa
    74821 gcgacgtttt gataaaaaaa tattgtcccc gaagtgatgg gaaaagtctt agcggcaatg
    74881 cagaatagaa agtgctcatc ttttatttcc cgggagggga aatgtcccgc gtatcgcagg
    74941 cgaggaacct gggtaaatat ttcctgctca tcgataatat gctggtcgtg ctggggttct
    75001 ttgttgtctt cccgctgatc tctatccgct tcgttgatca aatgggctgg gccgccgtca
    75061 tggtcggtat tgctctcggt ctacgccagt ttattcagca aggtctgggt attttcggcg
    75121 gcgcaattgc cgaccgcttt ggtgccaaac cgatgattgt taccggtatg ctgatgcgcg
    75181 ccgccggatt cgccacaatg ggtatcgccc acgaaccgtg gctattgtgg ttttcatgcc
    75241 tgctctcggg actcggcggc acgttgtttg atccgccgcg ttcggcgctg gtggtgaaac
    75301 taatccgtcc acaacaacgt ggtcgttttt tctcgctgtt gatgatgcag gacagtgcca
    75361 gtgcggtcat tggcgcattg ttggggagct ggctgttgca atacgacttt cgcctggtct
    75421 gcgccacagg ggccgttttg tttgtgctat gtgcggcgtt caatgcgtgg ttgttaccgg
    75481 catggaaact ctccaccgta cgcacgcccg ttcgcgaagg catgacccgc gtgatgcgtg
    75541 acaagcgttt tgtcacctat gtcctgacgc tggcgggtta ctacatgctg gctgtacaag
    75601 tgatgctgat gctgccaatt atggtcaacg acgtggctgg cgcgccctct gccgttaaat
    75661 ggatgtatgc cattgaagcg tgtctgtcgt taacgttgct ctaccctatc gcccgctgga
    75721 gtgaaaagca ttttcgtctg gaacaccggt tgatggctgg gctgttgata atgtcattaa
    75781 gcatgatgcc ggtgggcatg gtcagcggcc tacaacaact tttcaccctg atttgtctgt
    75841 tttatatcgg gtcgatcatt gccgagcctg cgcgtgaaac cttaagtgct tcgctggcgg
    75901 acgcaagagc tcgcggcagc tatatggggt ttagccgtct gggtctggcg attggcggcg
    75961 ctattggtta tatcggtggt ggctggctgt ttgacctggg caaatcgacg caccagccag
    76021 agcttccgtg gatgatgctg ggaattattg gcatcttcac tttccttgcg ctgggttggc
    76081 agtttagtca gaaacgcgcc gcgcgtcgtt tgctggaacg cgacgcctga tttgatacac
    76141 ccccctgact cctccatact gaaagtgaac tgacagccaa atggaggagt cacgtgaagc
    76201 tatacattta cgatcactgc ccttactgcc tcaaagcccg catgattttc ggcctgaaaa
    76261 atatccccgt cgaattacat gttctgctca acgacgacgc agaaacaccc acccggatgg
    76321 tcggtcaaaa acaggttccc attctgcaaa aagatgacag ccgctatatg ccagaaagca
    76381 tggatatcgt tcactatgtc gataaactcg acggcaaacc gttactgacc ggcaaacgtt
    76441 cccctgccat tgaagagtgg ctgcgcaagg tcaatggcta cgccaacaaa ctgctgttgc
    76501 cgcgttttgc caaatcggca tttgatgagt tttctactcc cgccgcgcgc aaatatttcg
    76561 tcgacaagaa agaggccagc gcgggtaatt ttgccgacct gctggcccac tctgacggtc
    76621 tgattaagaa tatcagcgat gatttgcgtg cgctggacaa actgatcgtc aaaccgaacg
    76681 ccgtgaatgg cgaactttcg gaagatgata ttcagctatt tccgctactg cgtaatctga
    76741 cgctggtagc cggaatcaac tggccaagcc gcgttgctga ttaccgcgat aatatggcga
    76801 aacagacaca aatcaatttg ttatcatcaa tggcgattta atcctgaccc gccgcggcag
    76861 cctgtattcg cgggttgccg ccgttttcag ttttttctgc tggcgagttt atcgctggtg
    76921 acacatgctg cgttgtgtca ggatattgac gcagatttca tgtcgaggac taccatgaac
    76981 aagtttttat ttgccgctgc attgatcgtc agtggcctgc tcgttggctg taatcaactc
    77041 acccaataca ccatcaccga acaagaaatt aaccagtcgc ttgcgaaaca taataatttc
    77101 tcaaaagata tcggtttacc cggcgtggct gacgcccata ttgttctgac aaacttgacc
    77161 agccaaattg gtcgcgaaga gccgaataag gttaccctaa ccggagacgc caatctggac
    77221 atgaactccc tgttcggtag tcagaaagcg accatgaaac tgaagctgaa agcgctgccc
    77281 gtgtttgata aagaaaaagg tgcgatcttc ctgaaagaga tggaagtggt cgatgcgacg
    77341 gtacaaccgg aaaaaatgca aacggtgatg caaacgttgc ttccctattt gaaccaggca
    77401 ttacgcaatt actttaacca gcaacctgct tacgtcctgc gcgaagatgg cagtcagggc
    77461 gaagcaatgg cgaaaaaact ggcgaaaggc attgaagtga agccaggcga aattgtcatt
    77521 ccgtttacgg attaatgacg agggcgcatt cgcgcccttt atttttcgtg caaaggaaaa
    77581 catttccgct tatcctttgt gtccggcaaa aacatccctt cagccggagc atagagatta
    77641 atgactgcac catcccaggt attaaagatc cgccgtccag acgactggca ccttcacctc
    77701 cgcgatggcg acatgttaca aactgtcgtg ccgtatacca gcgaaattta tggacgggct
    77761 atcgtaatgc ccaatctggc tccgcccgtg accacagttg aggctgccgt ggcgtatcgc
    77821 caacgtattc ttgacgccgt acctgccggg cacgatttca ccccattgat gacctgttat
    77881 ttaacagatt cgctggatcc taatgagctg gagcgcggat ttaatgaagg cgtgttcacc
    77941 gctgcaaaac tttacccggc aaacgcaacc actaactcca gccacggcgt gacgtcaatt
    78001 gacgcaatca tgccggtact tgagcgcatg gaaaaaatcg gtatgccgct actggtgcat
    78061 ggtgaagtga cacatgcaga tatcgacatt tttgatcgtg aagcgcgctt tatagaaagc
    78121 gtgatggaac ctctgcgcca gcgcctgact gcgctgaaag tcgtttttga gcacatcacc
    78181 accaaagatg ctgccgacta tgtccgtgac ggaaatgaac ggctggctgc caccatcact
    78241 ccgcagcatc tgatgtttaa ccgcaaccat atgctggttg gtggcgtgcg tccgcacctg
    78301 tattgtttac ccatcctcaa acgcaatatt caccaacagg cattgcgtga actggtcgcc
    78361 agcggtttta atcgagtatt cctcggtacg gattctgcgc cacatacacg tcatcgcaaa
    78421 gagagcagtt gcggctgcgc gggctgcttc aacgccccaa ccgcgctggg cagttacgct
    78481 accgtctttg aagagatgaa tgctctgcag cactttgaag cattctgttc tgtaaacggc
    78541 ccgcagttct atggcttgcc ggtcaacgac acattcatcg aactggtacg tgaagagcaa
    78601 caggttgctg aaagcatcgc actgactgat gacaccctgg tgccattcct cgccggggaa
    78661 acggtacgct ggtccgttaa acaataaaaa tcgtagcttc ctgttgtcat taggttattt
    78721 tacctgtata aataaccagt atattcaaca gggggctatt atgcgaattg aagtcaccat
    78781 agcgaaaact tctccattgc cagctggggc tattgacgcc ctggctggcg aactttcccg
    78841 ccgtattcag tatgcgtttc ctgataatga aggccacgta tcggtacgtt atgccgcagc
    78901 gaataattta tcggttattg gcgcaacaaa agaagataaa cagcgcatta gcgaaattcc
    78961 ccaggaaacg tgggaaagcg ccgatgactg gtttgtcagc gaataatatg cagtgatttt
    79021 tgtttttgcc gggtcgcccc ggctttttta ttttacttac gaattttgag tacaaaattt
    79081 ctacacttcc tcaaaataat tgcacaatat gctaaaaaaa tatgaatgac atgctgtttt
    79141 attgttcata taaatcttaa aagcagaaat ttatctgttc ccccctttaa aaattcggtg
    79201 aataccctta cttattggta tactgaagtt gcttcagata aaaacgcatt gaacctcgaa
    79261 taacgttgtc tagtaacacg aattaggggg ccatgatgga aaaaaataat gaagtcattc
    79321 agactcatcc gctcgtaggg tgggacatca gcaccgttga tagctatgat gcgctgatgt
    79381 tgcgtttgca ctaccagacc ccaaataagt ccgagcagga agggactgaa gttggtcaga
    79441 cgctctggtt aaccactgat gttgccagac aatttatttc gatattagaa gcaggaatcg
    79501 ccaaaattga atccggtgat ttcccggtaa acgagtatcg gcgtcattaa catgacatga
    79561 ccatccaccc aaaaggcacc tcaccggtgc ctttaccatt tctgggttgt atatctgcat
    79621 tcagttaatt actctgctta aaccatgaga gcagagagaa aaaaatgaaa tacgatctca
    79681 tcattattgg cagcggttcc gtaggcgctg ccgccgggta ttatgcaacc cgcgccggtt
    79741 taaacgtgct aatgaccgac gcccatatgc caccgcatca acacggcagc caccacggcg
    79801 atacgcgatt aattcgccat gcttatggtg aaggcgaaaa gtatgtcccg ttagtcctcc
    79861 gcgcgcaaac gctgtgggat gaactttccc gccacaacga agatgatccc atttttgtac
    79921 gctctggtgt cattaacctc ggcccggctg actccgcatt tctcgccaac gtcgcccaca
    79981 gcgccgaaca gtggcaactc aacgttgaaa agctcgatgc gcaagggatt atggcccgct
    80041 ggccagaaat acgcgtcccg gacaactaca tcggcttatt tgagactgat tccggttttt
    80101 tgcgcagcga actggcgatt aaaacctgga tccaactggc gaaggaagcg ggctgtgcgc
    80161 aactgttcaa ctgcccggtc accgcaattc gtcatgacga tgatggcgta actattgaaa
    80221 cggttgacgg tgagtatcag gcgaaaaaag cgattgtctg cgcgggaaca tgggtaaaag
    80281 acctgctccc ggagctgcct gtccagcccg tacgtaaagt atttgcctgg tatcaggccg
    80341 atggccgcta tagcgtgaag aataaattcc cggcgtttac cggtgaactg cccaatggcg
    80401 atcaatatta tggttttccg gcagaaaacg acgcgttgaa gattggcaaa cataacggag
    80461 gccaggttat ccattcagcg gatgaacgtg ttccgtttgc ggaagtggcc agtgatgggt
    80521 cagaagcctt cccgttcttg cgcaatgtat tgccgggtat cggttgctgc ctgtacggcg
    80581 ctgcctgcac ctatgataat tcgcctgacg aagattttat tatcgatacc ctacccggcc
    80641 acgataatac actgctcatt accggcctga gtgggcacgg ttttaaattt gcgtcagttt
    80701 taggggaaat agctgccgat tttgcgcaag accaaaaaag cgattttgat ttgacgccat
    80761 tcaggctttc ccgcttccaa taatcatcat tacggcctcc agaatgaggc cgtttttaca
    80821 ggagtattta atgcgtcggc tgttgcacta tctcatcaat aatattcgcg agcatctgat
    80881 gctttatctt tttctctggg gattgttggc aattatggat ctgatctatg tgttttattt
    80941 ttagttttcc gcactgacat aaactgacaa atatttaatc tccctttcat ttagcgtgaa
    81001 aaattgaatg agattattcc tgcttctaca gcactcacca gacatctctg atgctgaacc
    81061 cactaaactt atgaagattc tttcattatt aattcaaaat tattgaaata aaaataaata
    81121 tcattcaatt tgttattttt gtgttgcaat tcattctccg acacgccatt ttctttctct
    81181 cgcaaattcg tattggatgc acttatgtca ttcacaaata cccctgaacg ctatggtgtt
    81241 atatctgcgg ccttccattg gctaagcgca attattgtct acggcatgtt tgctttaggt
    81301 ctgtggatgg tcacgctcag ttattacgat ggctggtatc acaaagcacc cgaactgcat
    81361 aaaagcatcg gtattttgtt aatgatgggg ctggttattc gcgttctgtg gcgtgtcata
    81421 tctccaccgc cgggtccgct gccgagttat tcgcccatga ctcgtcttgc cgcaaaagct
    81481 ggacatctcg cattgtacct attgctgttc gccatcggca tcagcggcta tctgatctca
    81541 actgccgatg gtaaaccgat cagcgttttt ggctggtttg acgtccccgc gacccttgcc
    81601 gacgctggcg cacaggcaga ctttgccggt gccctgcatt tttggctcgc gtggagcgtc
    81661 gtagtactgt ctgttatgca cggatttatg gccctgaagc atcatttcat cgataaagac
    81721 gacactctga agcgcatgct gggaaagtcg tcatctgact atggagtata aaaaatgaaa
    81781 aaaagcctgc ttggtttaac cttcgcgtcc ctgatgttct ctgccggttc agcggttgcc
    81841 gccgattaca aaattgacaa agaaggtcag cacgcctttg ttaacttccg catacagcat
    81901 cttggctata gctggttata tggcaccttt aaagatttcg acggtacttt tacctttgat
    81961 gaaaaaaatc cggctgccga taaagtgaat gtgacaatta acaccaccag cgtcgatact
    82021 aaccacgccg aacgcgataa acatcttcgc agtgcggatt ttctcaatac cacaaaatat
    82081 ccacaggcaa cattcacctc caccagcgtg aagaaagacg gtgacgaact ggatattacc
    82141 ggcgatctga cgctgaatgg cgtaaccaaa cccgtcacgc tggaagcgaa attaattggt
    82201 cagggcgacg acccatgggg cggtaaacgt gcaggctttg aggccgaagg caaaattaag
    82261 ctcaaagact tcaatatcaa gacagattta ggtccagctt ctcaggaagt ggatctgatt
    82321 atttcagtgg aaggcgtaca gcagaagtaa gagtgaaatg ttgccggatg gcacgcatcc
    82381 ggcaatgata tttattctgt tggatcagga atacccagtg ttgtattcag acgtccacga
    82441 gacttattaa agatcttatt gccattttca cgtcctgccc gacggcgtcg ctgttcttct
    82501 ggcggtaacg cgctttcttc gcagcaaatc tcactacaac aacctttgta tttttccgcg
    82561 catactggac actgaataaa cagcaggtgg cagccatcat ttttacagtt ggtatggctg
    82621 tcgcacggcg cgccgcactg gtggcaatgc gcgataatct catccgatat acgttcgccc
    82681 atccgctcgt caaaaacaaa atttttgcca ataaaacgca ccggcaagcc ctgctcgcgc
    82741 gccttacggg cgtattcaat aattccgccc tcgatatgcc agactttgtt gaatccgtta
    82801 tgtttcatcc aggcactggc cttttcacaa cgaatgccgc cggtgcagta catgacgatt
    82861 tttttatctt tatgtgcctg catcatctcg actgcttttg gcagctgctc acggaaggta
    82921 tctgccggaa tttcgagcgc gttttcaaag tgccccactt catactcata gtggttacgc
    82981 atgtcgataa atagtgcatc gggatcgtca agcatggcgt tcacttccgc cgcttgcaga
    83041 tactcaccaa cattgctggc atcaaagtga ggatcgtcaa taccgtcggc aacaatgcga
    83101 tcgcgtacct tcatgcgcag tacccagaag gatttcccgt catcatccaa cgcgatattc
    83161 aggcgtaaac cctctaaagc cgggtcgaag gcatagagct gcgcgcgaaa tgtttcaaca
    83221 ttgctcgcag gtacgctgat ttgcgcgtta atgccctcat gcgccagata cactcgccca
    83281 aaaacattca gcgcggtaaa cagctgatat aaagcgtcac gggtcgcctt aggatcggcg
    83341 atgtggaaat acttgtaaaa cgaaatggtg gttcgcggtt cgctctcagc caacattttg
    83401 gcttttaacg cgtcgttgga aatgcggttg tgtaacactg gcatggtgta cggttcctgc
    83461 gagatgggaa agtaaaaatc cgcggcatga tatagcaatt atcgataatt aacatccaca
    83521 cattttacgc tacatttgcg cattaaaaat tatttgttat ttacaagcgc ggcaatttcg
    83581 cccagtcttc agccacaaat tttggttgcg ggcgaaaaaa tgcgacaata catacaattg
    83641 cccgtatagg ttgaaaaaca ggattgatat gacgaatcta cccaagttct ccaccgcact
    83701 gcttcatccg cgttattggt taacctggtt gggtattggc gtactttggt tggtcgtgca
    83761 attgccctac ccgattatct accgcctcgg ttgtggatta ggaaaactgg cgttacgttt
    83821 tatgaaacga cgcgcaaaaa ttgtgcatcg caacctggaa ctgtgcttcc cggaaatgag
    83881 cgaacaagaa cgccgtaaaa tggtggtgaa gaatttcgaa tccgttggca tgggcctgat
    83941 ggaaaccggt atggcgtggt tctggccgga ccgccgaatc gcccgctgga cggaagtgat
    84001 cggcatggaa cacattcgtg acgtgcaggc gcaaaaacgc ggcatcctgt tagttggcat
    84061 ccattttctg acactggagc tgggcgcgcg gcagtttggt atgcaggaac cggggattgg
    84121 cgtttaccgc ccgaacgata atccactgat tgactggcta caaacctggg gccgtttgcg
    84181 ctcaaataaa tcgatgctcg accgcaaaga tttaaaaggc atgattaaag ccctgaaaaa
    84241 aggcgaagtg gtctggtacg caccagatca tgattacggc ccgcgctcaa gcgttttcgt
    84301 cccgttattt gccgttgagc aggctgcgac cacgaccgga acctggatgc tggcacggat
    84361 gtccggcgca tgtctggtgc ccttcgttcc acgccgtaag ccagatggca aagggtatca
    84421 attgattatg ctgccgccag agtgttctcc gccactggat gatgccgaaa ctaccgccgc
    84481 gtggatgaac aaagtggtcg aaaaatgcat catgatggca ccagagcagt atatgtggtt
    84541 acaccgtcgc tttaaaacac gcccggaagg cgttccttca cgctattaaa tctcccatgc
    84601 cggatgcttc agaatggcat ccggcattac cacagcaaat ccccctgatt tagcgataaa
    84661 agctctctgg attgcgcccc ctggaagtcg ggcgcataat tagtgtgttt atcttttctt
    84721 cttatgttca ccgcgcctgg cgcaccaaca gcggattgct atgtcaccct gtgaaaatga
    84781 cacccctata aactggaaac gaaacctgat cgtcgcctgg ctaggctgtt ttcttaccgg
    84841 ggccgccttc agtctggtaa tgcccttctt acccctctac gttgagcagc ttggtgttac
    84901 cggccactcc gccctgaata tgtggtccgg tattgtcttc agtataatgg gttcctaaac
    84961 acatcatttt agatattgaa tatgagacgg gcaatcagct acatcagatt ttcaagtgaa
    85021 cgacaactga aaggggactc tgtacgccga caatctaagc tggttactga ctggcttgat
    85081 aagaaccctg agttttactg ggattcatcc ttgagcttta aagatttggg gaaaagtgct
    85141 tttagtggta agcacctgaa aggtggtcta ggtgactttt taaccgctat cgagaaaggg
    85201 cttgttaaag ctggtgatac gctactaatc gagagtttag accgtctgtc acgtcaggat
    85261 attgatattg cttctgagct attacgccgt atcttaaggg ctggtgtaga tgtagtcaca
    85321 ctgagcgatg gagaacatta cacacgagaa agtttaaaag acccgttagc cctgattaaa
    85381 agcattctga taatgcagcg ggcgcatgag gaaagtttga ggaagtctga gcgtgtccaa
    85441 gctgcatgga accgtaaaaa agaactcatc tccgaaggta tcaaggtatc taggcgttgt
    85501 cctgcatggt taaggctgaa tgatgaccgt cgaacattca caatcattcc tgataaagtg
    85561 gaagttgtta aacgagcatt tgacctgaga ttacagggtt taagcttttg ggctatcacc
    85621 agaactttaa acgatgaagg tcacttgtca ttgaatcaat acacacccaa acaaaagggg
    85681 tggtctgata ctgctgtaaa gaaactactc agaaatcgtg ctgtgattgg atgtttcaca
    85741 cctgctggac gtgaagaagt tcagggttac taccccgcaa taatcagtga gtcactattc
    85801 tatcgagtac agcaactgaa cacagggcaa tatggtaggg cttctgtatc aagtaatccg
    85861 ctatcagtca acttgttcag gggtatcatc aaatgttctg agtgtggggc tactattgtt
    85921 cttggtggat atgccttaaa acgttttgga atgtacagat gccctaatcg ttcagctaac
    85981 aggtgtagtg caaaggctat cagtcgtaag caaacggatg caactatcct ttacatgctc
    86041 gcattatgcg accgttttga aactgaaaat actgacacta ttgatagcct taaattacag
    86101 cgtgaagatt tgca
//
LOCUS       DAETPZ010000004        76413 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.004, whole genome shotgun sequence.
ACCESSION   DAETPZ010000004 DAETPZ010000000
VERSION     DAETPZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 76413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.004"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..545
                     /locus_tag="K0W59_000345"
     CDS             <1..545
                     /locus_tag="K0W59_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HBI3021520.1"
                     /translation="FPINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGHDKEN
                     SLKRVINSFNSELMGEMSNNNIKVHLNEPEIIFLHADLQQYLSQSCGAFVCMAAQEVI
                     EQRESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASY
                     TQLEIKHSHPKNRASGKRVS"
     gene            965..1580
                     /locus_tag="K0W59_000346"
                     /pseudo
     CDS             965..1580
                     /locus_tag="K0W59_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1683..2186
                     /locus_tag="K0W59_000347"
     CDS             1683..2186
                     /locus_tag="K0W59_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBI3021521.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLDEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(2253..3710)
                     /gene="nuoN"
                     /locus_tag="K0W59_000348"
     CDS             complement(2253..3710)
                     /gene="nuoN"
                     /locus_tag="K0W59_000348"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HBI3021522.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3717..5246)
                     /gene="nuoM"
                     /locus_tag="K0W59_000349"
     CDS             complement(3717..5246)
                     /gene="nuoM"
                     /locus_tag="K0W59_000349"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HBI3021523.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITVATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(5410..7251)
                     /gene="nuoL"
                     /locus_tag="K0W59_000350"
     CDS             complement(5410..7251)
                     /gene="nuoL"
                     /locus_tag="K0W59_000350"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HBI3021524.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(7248..7550)
                     /gene="nuoK"
                     /locus_tag="K0W59_000351"
     CDS             complement(7248..7550)
                     /gene="nuoK"
                     /locus_tag="K0W59_000351"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HBI3021525.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7547..8101)
                     /gene="nuoJ"
                     /locus_tag="K0W59_000352"
     CDS             complement(7547..8101)
                     /gene="nuoJ"
                     /locus_tag="K0W59_000352"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HBI3021526.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(8113..8655)
                     /gene="nuoI"
                     /locus_tag="K0W59_000353"
     CDS             complement(8113..8655)
                     /gene="nuoI"
                     /locus_tag="K0W59_000353"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HBI3021527.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8670..9647)
                     /gene="nuoH"
                     /locus_tag="K0W59_000354"
     CDS             complement(8670..9647)
                     /gene="nuoH"
                     /locus_tag="K0W59_000354"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HBI3021528.1"
                     /translation="MSWISPELIEILLTILKAVGILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9644..12370)
                     /gene="nuoG"
                     /locus_tag="K0W59_000355"
     CDS             complement(9644..12370)
                     /gene="nuoG"
                     /locus_tag="K0W59_000355"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HBI3021529.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(12423..13760)
                     /gene="nuoF"
                     /locus_tag="K0W59_000356"
     CDS             complement(12423..13760)
                     /gene="nuoF"
                     /locus_tag="K0W59_000356"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBI3021530.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13757..14257)
                     /gene="nuoE"
                     /locus_tag="K0W59_000357"
     CDS             complement(13757..14257)
                     /gene="nuoE"
                     /locus_tag="K0W59_000357"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HBI3021531.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(14260..16062)
                     /gene="nuoC"
                     /locus_tag="K0W59_000358"
     CDS             complement(14260..16062)
                     /gene="nuoC"
                     /locus_tag="K0W59_000358"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HBI3021532.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRTQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(16156..16818)
                     /gene="nuoB"
                     /locus_tag="K0W59_000359"
     CDS             complement(16156..16818)
                     /gene="nuoB"
                     /locus_tag="K0W59_000359"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HBI3021533.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16834..17277)
                     /gene="nuoA"
                     /locus_tag="K0W59_000360"
     CDS             complement(16834..17277)
                     /gene="nuoA"
                     /locus_tag="K0W59_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HBI3021534.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRECMNPETNSIANRQR"
     gene            complement(17908..18846)
                     /gene="lrhA"
                     /locus_tag="K0W59_000361"
     CDS             complement(17908..18846)
                     /gene="lrhA"
                     /locus_tag="K0W59_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HBI3021535.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19776..20993
                     /gene="alaA"
                     /locus_tag="K0W59_000362"
     CDS             19776..20993
                     /gene="alaA"
                     /locus_tag="K0W59_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HBI3021536.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            21077..21676
                     /gene="yfbR"
                     /locus_tag="K0W59_000363"
     CDS             21077..21676
                     /gene="yfbR"
                     /locus_tag="K0W59_000363"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HBI3021537.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21735..23567)
                     /locus_tag="K0W59_000364"
     CDS             complement(21735..23567)
                     /locus_tag="K0W59_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBI3021538.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23654..24304)
                     /gene="hxpA"
                     /locus_tag="K0W59_000365"
     CDS             complement(23654..24304)
                     /gene="hxpA"
                     /locus_tag="K0W59_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HBI3021539.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(24315..24809)
                     /locus_tag="K0W59_000366"
     CDS             complement(24315..24809)
                     /locus_tag="K0W59_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HBI3021540.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24892..25347)
                     /locus_tag="K0W59_000367"
     CDS             complement(24892..25347)
                     /locus_tag="K0W59_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HBI3021541.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25685..26887
                     /gene="ackA"
                     /locus_tag="K0W59_000368"
     CDS             25685..26887
                     /gene="ackA"
                     /locus_tag="K0W59_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBI3021542.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26962..29106
                     /gene="pta"
                     /locus_tag="K0W59_000369"
     CDS             26962..29106
                     /gene="pta"
                     /locus_tag="K0W59_000369"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBI3021543.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            29296..30816
                     /gene="yfcC"
                     /locus_tag="K0W59_000370"
     CDS             29296..30816
                     /gene="yfcC"
                     /locus_tag="K0W59_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBI3021544.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30849..31391)
                     /gene="yfcD"
                     /locus_tag="K0W59_000371"
     CDS             complement(30849..31391)
                     /gene="yfcD"
                     /locus_tag="K0W59_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HBI3021545.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(31449..32000)
                     /gene="yfcE"
                     /locus_tag="K0W59_000372"
     CDS             complement(31449..32000)
                     /gene="yfcE"
                     /locus_tag="K0W59_000372"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBI3021546.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(32056..32700)
                     /gene="yfcF"
                     /locus_tag="K0W59_000373"
     CDS             complement(32056..32700)
                     /gene="yfcF"
                     /locus_tag="K0W59_000373"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HBI3021547.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32836..33483
                     /gene="yfcG"
                     /locus_tag="K0W59_000374"
     CDS             32836..33483
                     /gene="yfcG"
                     /locus_tag="K0W59_000374"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HBI3021548.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33540..33902
                     /gene="folX"
                     /locus_tag="K0W59_000375"
     CDS             33540..33902
                     /gene="folX"
                     /locus_tag="K0W59_000375"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HBI3021549.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIVREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33923..34816
                     /locus_tag="K0W59_000376"
     CDS             33923..34816
                     /locus_tag="K0W59_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HBI3021550.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSHWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34864..35754)
                     /gene="rpnB"
                     /locus_tag="K0W59_000377"
     CDS             complement(34864..35754)
                     /gene="rpnB"
                     /locus_tag="K0W59_000377"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HBI3021551.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35951..36724)
                     /gene="hisP"
                     /locus_tag="K0W59_000378"
     CDS             complement(35951..36724)
                     /gene="hisP"
                     /locus_tag="K0W59_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HBI3021552.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36732..37448)
                     /locus_tag="K0W59_000379"
     CDS             complement(36732..37448)
                     /locus_tag="K0W59_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3021553.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(37445..38131)
                     /gene="hisQ"
                     /locus_tag="K0W59_000380"
     CDS             complement(37445..38131)
                     /gene="hisQ"
                     /locus_tag="K0W59_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HBI3021554.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     LGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(38221..39003)
                     /gene="hisJ"
                     /locus_tag="K0W59_000381"
     CDS             complement(38221..39003)
                     /gene="hisJ"
                     /locus_tag="K0W59_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HBI3021555.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(39224..40006)
                     /gene="argT"
                     /locus_tag="K0W59_000382"
     CDS             complement(39224..40006)
                     /gene="argT"
                     /locus_tag="K0W59_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HBI3021556.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALLPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(40272..40841)
                     /gene="ubiX"
                     /locus_tag="K0W59_000383"
     CDS             complement(40272..40841)
                     /gene="ubiX"
                     /locus_tag="K0W59_000383"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HBI3021557.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40936..42453)
                     /gene="purF"
                     /locus_tag="K0W59_000384"
     CDS             complement(40936..42453)
                     /gene="purF"
                     /locus_tag="K0W59_000384"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBI3021558.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEEENLEMHNEG"
     gene            complement(42490..42978)
                     /gene="cvpA"
                     /locus_tag="K0W59_000385"
     CDS             complement(42490..42978)
                     /gene="cvpA"
                     /locus_tag="K0W59_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HBI3021559.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(43237..43899)
                     /gene="dedD"
                     /locus_tag="K0W59_000386"
     CDS             complement(43237..43899)
                     /gene="dedD"
                     /locus_tag="K0W59_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HBI3021560.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43889..45157)
                     /gene="folC"
                     /locus_tag="K0W59_000387"
     CDS             complement(43889..45157)
                     /gene="folC"
                     /locus_tag="K0W59_000387"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBI3021561.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIELGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGRELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADAAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(45227..46141)
                     /gene="accD"
                     /locus_tag="K0W59_000388"
     CDS             complement(45227..46141)
                     /gene="accD"
                     /locus_tag="K0W59_000388"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HBI3021562.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(46297..46956)
                     /locus_tag="K0W59_000389"
     CDS             complement(46297..46956)
                     /locus_tag="K0W59_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI3021563.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(47039..47851)
                     /gene="truA"
                     /locus_tag="K0W59_000390"
     CDS             complement(47039..47851)
                     /gene="truA"
                     /locus_tag="K0W59_000390"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBI3021564.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47851..48864)
                     /locus_tag="K0W59_000391"
     CDS             complement(47851..48864)
                     /locus_tag="K0W59_000391"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBI3021565.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQLWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48930..50066)
                     /gene="pdxB"
                     /locus_tag="K0W59_000392"
     CDS             complement(48930..50066)
                     /gene="pdxB"
                     /locus_tag="K0W59_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HBI3021566.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            50165..51160
                     /gene="flk"
                     /locus_tag="K0W59_000393"
     CDS             50165..51160
                     /gene="flk"
                     /locus_tag="K0W59_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HBI3021567.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMPTRPALWILLVAIILMLVWLVR"
     gene            complement(51157..52335)
                     /locus_tag="K0W59_000394"
     CDS             complement(51157..52335)
                     /locus_tag="K0W59_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HBI3021568.1"
                     /translation="MTAVSQTETRSSASFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52610..53830)
                     /gene="fabB"
                     /locus_tag="K0W59_000395"
     CDS             complement(52610..53830)
                     /gene="fabB"
                     /locus_tag="K0W59_000395"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HBI3021569.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDITGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53989..55995
                     /gene="mnmC"
                     /locus_tag="K0W59_000396"
     CDS             53989..55995
                     /gene="mnmC"
                     /locus_tag="K0W59_000396"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HBI3021570.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAR"
     gene            complement(56116..56394)
                     /locus_tag="K0W59_000397"
     CDS             complement(56116..56394)
                     /locus_tag="K0W59_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HBI3021571.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(56428..56976)
                     /gene="epmC"
                     /locus_tag="K0W59_000398"
     CDS             complement(56428..56976)
                     /gene="epmC"
                     /locus_tag="K0W59_000398"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HBI3021572.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56976..57785)
                     /locus_tag="K0W59_000399"
     CDS             complement(56976..57785)
                     /locus_tag="K0W59_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBI3021573.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57785..58609)
                     /gene="mepA"
                     /locus_tag="K0W59_000400"
     CDS             complement(57785..58609)
                     /gene="mepA"
                     /locus_tag="K0W59_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HBI3021574.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSPDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58613..59698)
                     /gene="aroC"
                     /locus_tag="K0W59_000401"
     CDS             complement(58613..59698)
                     /gene="aroC"
                     /locus_tag="K0W59_000401"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBI3021575.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKEGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59733..60665)
                     /gene="prmB"
                     /locus_tag="K0W59_000402"
     CDS             complement(59733..60665)
                     /gene="prmB"
                     /locus_tag="K0W59_000402"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBI3021576.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            60831..61382
                     /gene="smrB"
                     /locus_tag="K0W59_000403"
     CDS             60831..61382
                     /gene="smrB"
                     /locus_tag="K0W59_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HBI3021577.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(61486..62376)
                     /locus_tag="K0W59_000404"
     CDS             complement(61486..62376)
                     /locus_tag="K0W59_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HBI3021578.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(62363..62887)
                     /locus_tag="K0W59_000405"
     CDS             complement(62363..62887)
                     /locus_tag="K0W59_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3021579.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62884..63354)
                     /locus_tag="K0W59_000406"
     CDS             complement(62884..63354)
                     /locus_tag="K0W59_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3021580.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDIAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(63351..63899)
                     /locus_tag="K0W59_000407"
     CDS             complement(63351..63899)
                     /locus_tag="K0W59_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3021581.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63904..64626)
                     /locus_tag="K0W59_000408"
                     /pseudo
     CDS             complement(63904..64626)
                     /locus_tag="K0W59_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64646..65368)
                     /locus_tag="K0W59_000409"
     CDS             complement(64646..65368)
                     /locus_tag="K0W59_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBI3021582.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(65428..67287)
                     /locus_tag="K0W59_000410"
     CDS             complement(65428..67287)
                     /locus_tag="K0W59_000410"
                     /inference="COORDINATES: protein motif:HMM:NF011812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HBI3021583.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYARRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGTMAVGRTAVRSVISAVSMTRLTIS"
     gene            complement(67369..67932)
                     /locus_tag="K0W59_000411"
     CDS             complement(67369..67932)
                     /locus_tag="K0W59_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBI3021584.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(68616..69101)
                     /gene="sixA"
                     /locus_tag="K0W59_000412"
     CDS             complement(68616..69101)
                     /gene="sixA"
                     /locus_tag="K0W59_000412"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HBI3021585.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(69304..71448)
                     /gene="fadJ"
                     /locus_tag="K0W59_000413"
     CDS             complement(69304..71448)
                     /gene="fadJ"
                     /locus_tag="K0W59_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HBI3021586.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(71448..72758)
                     /gene="fadI"
                     /locus_tag="K0W59_000414"
     CDS             complement(71448..72758)
                     /gene="fadI"
                     /locus_tag="K0W59_000414"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HBI3021587.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(72938..73222)
                     /locus_tag="K0W59_000415"
     CDS             complement(72938..73222)
                     /locus_tag="K0W59_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HBI3021588.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            73594..74934
                     /gene="fadL"
                     /locus_tag="K0W59_000416"
     CDS             73594..74934
                     /gene="fadL"
                     /locus_tag="K0W59_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HBI3021589.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLQADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            75300..75584
                     /locus_tag="K0W59_000417"
     CDS             75300..75584
                     /locus_tag="K0W59_000417"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3021590.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQ"
     gene            75677..76351
                     /locus_tag="K0W59_000418"
     CDS             75677..76351
                     /locus_tag="K0W59_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3021591.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGKSEPTDLF
                     TWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSENARS
                     ASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKF
                     RRDNVKEIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNCQAKYQNEINQLSALG
                     VNFDDI"
BASE COUNT        19317 a        20453 c        18740 g        17903 t
ORIGIN      
        1 tattccccat aaatactggc ggacactgga tattagtttc gctacagaaa atagtaaata
       61 aaaaaaataa taaactacaa ataaaatgcg tcatattcaa ctcattgcgt gcactaggcc
      121 atgataaaga aaattcactt aagcgtgtca ttaatagttt taattctgaa ctcatgggag
      181 aaatgtcgaa taataatata aaagttcatt taaatgaacc agagataata tttttacatg
      241 ccgatcttca gcaatactta agccaaagtt gcggtgcgtt tgtgtgcatg gcagcccagg
      301 aagtgattga acaaagggaa agcaattctg acagcgcccc ctatacgtta ttaaaaaact
      361 atgctgacag atttaaaaaa tattcagcag aagagcagta cgaaattgat tttcaacatc
      421 gactggtaaa cagaaattgt tatttagata aatatggcga tgcacgaatc aatgcttctt
      481 atacacaatt agaaataaaa cactcacacc ccaaaaatag agcatccggc aaaagagtga
      541 gttaacatca ttgttaatca attcgaccag tcagtaaata tgctgactgg tcgaacataa
      601 tttgctaaaa agtcatcact gactgatgtc agtcccacca tctgccagtt caatctacgg
      661 gtatttccag cgtatcgcgg cacgcgattg ctggtctgtt gatgtagtgg attgtagttg
      721 ctgacttttc ttaccctcag atttctgccc cacttgtcaa ttcaatctgc caataaataa
      781 cataattata tttttattcc tcacagaaat tcctttttaa aaatagcgaa cccactgtaa
      841 aaaacacttt ttgtatacct ctaccaagcg taataaaata aacgctcata attcattata
      901 aataatgatg aaataacgcc ttttattgat aatttacata tgaaaaaatt tttccttttg
      961 ttatattatt tctgttttca ctccctctga ttatttttac caaccctggg tcactgcatt
     1021 atcaccaacg ccccgacacg taagtcctga gcaattagaa aaaacagttc gttatcttac
     1081 acaaactgtg catccacgta atgccggcaa tattgataat ctgaataggt ccgcagagta
     1141 tataaaagag ttctttgtca gtagcggtgc cagagttacc gcgcagggcg tccccgattg
     1201 gctattacga ctctgcgcct ggaagccaga attaccctta cccggcaatg tcctggcttt
     1261 atcccgatcg gggagacttt attgctgtgg tcggcagaat gcaggatatc aacgccgttc
     1321 gtcaggtaaa agcggcattg ttgtcatccc gggatttatc tgtttattct atgaatgccc
     1381 cagggtttat tcccggtatt gatttttctg atcaccttaa ttattggcaa cacgatattc
     1441 ccgccgtaat gattactgac accgcttttt atcgtaataa acaataccac ttgcccggtg
     1501 ataccgcaga cagattgaat tatcagaaaa tggctcaggt ggtagatggt gttataacct
     1561 tgttatacaa cagtaaataa taaaaatttc tcgaggcggg tccagaaaca gcgacatgta
     1621 cattttattt tccctcgtct gttttaagat gcgaaacata aaattcaatg gaggaaaaat
     1681 aaatgggaat gattggctat tttgcagaaa ttgactcaga aaagattaac caactgcttg
     1741 agtctactga aaaacctttg atggataata tacatgacac gctttcagga ttacgccgac
     1801 tggatattga taaacgctgg gattttttac actttgggct gacaggcacc tctgcttttg
     1861 atcccgcgaa gaatgatccg ctgagtcgtg ccgtgcttga tgaacacagc ctcgaagatg
     1921 gcatagatgg cttcctggga ttgacctgga atcaggaatt agccgcgaca atcgaccgac
     1981 tggaatcact ggatcgaagt gagctgcgta aacagttttc cattaagcga ctcaatgaaa
     2041 tggagattta tccgggagtg accttttcag aagagttaga ggggcaactc tttgcctcga
     2101 tcatgcttga tatggaaaag ctgattagcg cgtatcgacg tatgctgcga caagggaatc
     2161 atgcgctgac ggttattgtt gggtgatggt gtcagagcgc caaaaaaaac cgccgaacat
     2221 gtcggcggct ttctgactta caaactaaca gtttacatca gcggcattgc taaccgcaca
     2281 atactaatca gcggttgtgg ccatacaccc agcaccagta ccaacagtgc agaaatcagc
     2341 accacgatac cgcccgcgct gtactgccag tttgacggtg catcgcgacc cggttgttcc
     2401 ggtgcgtgca ggtacaggct caccgccacg cgcaggtagt agtagaggcc gattgccgaa
     2461 ccgacaacca cggcacccac cagccaccac aagtgtgcct ggacaccgac cgccagcacg
     2521 tagaacttac cgataaagcc cagcgtcatc gggataccgg ccagagacag catcatcacc
     2581 gtcatcactg ccgcgaggat cggacgatgc cagaacagac cgcggtaaga gaacagggaa
     2641 tcagcatccg ggccacgata cgggctggac atcaggctga ccacgccgaa cgcgccgagg
     2701 ctgctgaaca gataaccggc caggtaaacc cctaccgctt ccatcgacat ctcgccggtt
     2761 tgcagcgcaa tcagcgccac cagcagatag ccgaggtgag agatagatga gtaaccgagc
     2821 agacgtttga tgttggtctg gctcaacgcc atcaggttac cgaagatgat ggaggcaaag
     2881 gcgataatcg ccagtactac gcgaatcgct tcgctgtcac ccaccggtgc gtagaggaac
     2941 agacgcatca ccacaccgaa gatagcgatt ttgctcgccg tcgccaggaa agtggaaacc
     3001 ggcgcaggcg cgccctggta tacgtctggc gtccacaggt ggaacggcac cagagagagt
     3061 ttgaagccga ggccaacaat catcatgccg aaacctgcca gcaacagcgg ctcgttgagc
     3121 ataccgtcgc cgaggttttt acccagcgcg acaaacgaca ggtcgccaga ctgcgcatac
     3181 accagcgcca taccgaacag caggaaagaa gacgctgcgg cagaaaggat ggtgtatttg
     3241 atactggctt ccagcgaacg tttctggcgg aaagcgtaac cgaccaggcc aaacagcggc
     3301 aaagagatca gttcaatacc gaggaacaga gacgccagat ggtttgcatt cgccagcagg
     3361 atcccgccca gcgcggcaat taacaccaac aggtagaact catccttgtt gtcgttatag
     3421 ccttcaagcc acgggtaggc gaaagtacag gtggcgaggc tcgccaacaa taccagcccg
     3481 gtgtaaagca tggcgaaacc atcaacgcgc atcagcggcg taacgtccat agcgcccgcc
     3541 tggccaacaa accagagcga aaccagcgcc gcgttaagcc caataaccga gagcgtagcg
     3601 ttgaggaaat gattgcgtcg ccacgcaatg gagagcatca caaccaccac cgtcaagccg
     3661 acgatcagca acggtagcag tgcgatcagg ttttgtggag ttattgtcat ggcgatttac
     3721 ggccttgtag tagtaacgga attaacaaac cactgctgga tattgccaat cgcggagtgc
     3781 gaggtatcca gaatcggctg cggatagaag cccagcagta ccagcagcac caccagcaac
     3841 aggatcataa acagctcacg cagcgacatc cctggcagtt cctggctggc aatctggctt
     3901 ttcgctttac cgaagtaagc gcgatgcaac atcgccagcg aataaacaga tgcaaagacc
     3961 agcccaaagg tagagataac ggtaatcacc gggacaacct ggaagctgcc gaacagaatc
     4021 ataaattcgc cgacgaagtt accggtgcca ggcatcccaa gcgttgccac cgcaaagaac
     4081 agcgacagtg ctggcagcca tttcatcttg ctccacagac cgcccatcat gcgcatgtcg
     4141 cgggtatgga tacgttcata aagctgaccg cagagaataa acagacccgc cgccgacaag
     4201 ccgtgcgcaa tcatctggat taccgcgccc tggtaggcca actggctgcc ggtgtagatt
     4261 gcaatcagca cgaagcccat gtgggaaacc gaggtgtagg cgatcagacg tttgatatcg
     4321 gtctgagcga aggccatcca cgcaccgtag aagatgccga taacacccag ccacatagcg
     4381 attggcgcga actctgccga cgcgttcggg aacagcggca gggagaaacg caacaaaccg
     4441 taagcggcgg ttttcagcaa gatccccgcg aggtccacgg aaccggcggt cggagcctgg
     4501 gagtgcgcat ccggcagcca gccatgcagc ggaaccaccg gcattttgac tgcgaaggcg
     4561 atgaagaagc ccagcatcaa cagatattcc acaccactgg acattggcgt attcagcagc
     4621 tcttcatagt tgaaggtcca gacgccggtc gcattgtagt gaacaaaaac cagcgccagg
     4681 atggcgatca acatcaccag accactcgcc tgggtgtaaa tgaagaactt ggttgccacc
     4741 gtgatacgcg ttttaccgtc agaggcttta tgcccccaca gtgcgatcag gaagtacatc
     4801 ggcaccagca tcatttccca gaagaagaag aacaggaaca tgtcgatggc aaggaacacg
     4861 ccgataacgc cgcccaggat ccacatcagg ttgaggtgga agaagccctg atatttttcg
     4921 atctctttcc acgaacatag taccgccagc acaccgagca gaccggtcag cacgaccatc
     4981 agcagcgaca gaccgtcaat ggcgagatga atagagatac caaaacgcgg gatccacggc
     5041 atgtcgaatt cagactgcca ctgcggaatt ccggcggatt gcgtcagtga ataaccgccc
     5101 tgcaaccaca gttgcagcga cagcgccagc gtcaatccca tggtgatcag cgcgatccag
     5161 cgtggcacct tgacgccaaa gcgttcggtc tgccagcaca ggaagccgcc aataaaggga
     5221 attaatatta gccagggtag taacatggcg atctttattc cttgtaaaag tcccgtcagg
     5281 accggatttt caacgaattc tcacgacaga aaattctctt cggactgggg gtagtgtcgg
     5341 atgcgccgcg aaggcagctt atccgaccta cgttcttata tgcccagggg caatcccaca
     5401 atccttaact caacgcagta ccatcaacag tgccagcacc acgaccgcac cgatgctcat
     5461 ggatgccaca taccagcgca gataaccgtt ctcgcttaac agcagacctt tacctgcaaa
     5521 gcgggaaaga accgccggga tgttcatcat tgagttcagc ggatcgcgtt tcagcaacca
     5581 ggcaataccc aggaacggct tgacgaacac tttgtcgtac agccagtcaa agccccaggc
     5641 gttgtaccac caggtactca gcaggcggcc cggcgcactg ttggcgatgg aggtcaccag
     5701 agtacgttta cccagccaca gccaggctgc cagcagaatg ccgaccaccg cgaccacgcc
     5761 agaggtaatt tccagggtca acatgctgcc gtgcgccagt tccgtcgttt gcggaagcac
     5821 gccctgcagc ggcggtacaa tcagtgcgcc aacgaaggtg gaaaggatca gcagcacaat
     5881 cagcggcagg ctgtgagtta cccctttcac ggcgtgagcg tgaatttgtt cttttccgtg
     5941 gaagacgatg aaaatcatac ggaaggtgta gagcgaggtc ataaacgcac cgaccagacc
     6001 tgccaccatc agattgatat gaccattcgc catcgcaccc gcgaggatct catccttact
     6061 gaagaagccc gcagtgacca gcggcagtgc cgacagtgct gcgccgccca ccaggaagca
     6121 gagataaacc agcggaatag atttacgcag accgcccatc ttgaagatgt tctgttcgtg
     6181 atggcaggcc agaatgacgg aaccggatgc caggaacagc agcgctttaa agaacgcgtg
     6241 agtcatcaag tggaaaatcg ccgcatccca tgcctgcacg ccaagcgcga ggaacatgta
     6301 gccaatctgg ctcatggtag agtaagcgag aacacgtttg atgtcggtct gtaccagcgc
     6361 ggcaaaaccg gccagcagca gcgtaaccgc cccgacaata cccaccagat gcagaacttc
     6421 cggcgtcatc aggaacaggc cgtgggtacg ggcgatcagg tagacacccg cggttaccat
     6481 cgttgcggcg tggatcagcg cggagacagg cgtcgggccc gccatcgcgt cagcaagcca
     6541 tgtctgcaac ggcaactgcg cagatttacc gaccgcaccg cccagcagca tcagcgtcgc
     6601 ccacatcagc atgttattgc cgtcagcaaa gtgcgctggt gccagttcca ccatttcgcg
     6661 gaagttcagg gtgcccagtt cgttgtaaag aatgaacagt gcgaaagcga ggaaaacgtc
     6721 acccacacgg gtcacgacga acgctttcat tgctgctgcg ccattcttcg gatcggtgta
     6781 atagaaaccg atcagcagat aggagcacag gcccacgcct tcccagccga ggtacatcag
     6841 cagcaggtta tcggcgagaa ccagaactac catgctggcg atgaacaggt tggtgtaggc
     6901 gaagaagcga gagtagccct cttcaccgcg catgtaccag gaggcgtaca tgtgaataag
     6961 gaaacccaca ccagtgacca ccgagagcat ggtcagcgac aggccgtcca gcaccaggtt
     7021 aaaaccgatg ttaaagtcgc ctaccgacat ccacgtccac agcggctggc tgtatgcctg
     7081 ctcgccgtta gcgaagaaat caacgccgat aaaggcggtt accagcgccg ccaggcccac
     7141 agagccaacg ccgacgatcg ccgaaacgtt ttcagaccag cgcccacggg agaacgccag
     7201 caggacgaag ccaatcaatg gcaaaataat ggttaaggca agcatgttca tccgcgcatc
     7261 tcacttactg aatcgatgtt caggttctgg cgacgacggt gaagttgcag cagcagcgca
     7321 aggccgatac tcgcttctgc cgccgcgagg ctgatggcga gaatgtacat cacctgaccg
     7381 tcggtctgac cccagtagct tccggcgacc acaaaggcca gcgcggaggc gttaatcatg
     7441 atttccagac caatcagcat aaacagcaga ttgcgacgga taaccagacc ggttaagcca
     7501 agaacgaata agattgccgc gaggatcagt ccatgttgta aggggatcat gcgtgctcct
     7561 ccgtttttct tttcgcgctg tcgtctttac gattgctcag cacttcaccc gcacgctctt
     7621 cacgaccgac gtggaaggcc acaaccagac ccgcgagcag cagcatagaa gccagttcca
     7681 ccgccagtac gtaaggcccg aacagcgtaa tacccactgc tttagcactg attggcgtac
     7741 cgtcgatacc ctgatcgtta acaccgagga tggcgtaaac aatcaccacc agcatgatgg
     7801 ccgacaaaat tgccgggcca atccacacct gcggtttcag ccactggcgt tcctgttcga
     7861 tttctgaacc gcccaggttg agcatcatca ccacgaacac gaacagcacc ataatggcac
     7921 ccgcgtagac gataatttcc agcgcaccgg cgaagtaagc gcctagtgag aagaacaccc
     7981 cggaaatcgc cagcagcgaa ataatcaggt acagcagtgc gtgtaccgga ttggtatggg
     8041 tgatcactcg caaggtcgca agtatggcta tcaggccaca gatataaaaa gcgaactcca
     8101 ttgcccctct ccttacggta acaggctctt gacgtcgata ggcttggctt cgttctctgc
     8161 ttcgccctta tctttgccgt cgattgccat acctgccatc cggtagaagt tatattccgg
     8221 gtatttgccc ggaccggaga tcagcagatc ctctttctcg taaaccagat cctggcgctt
     8281 gtattccccc atttcgaaat ccggggttaa ctgaatcgcc gtggtcggac aggcttcttc
     8341 gcacagacca cagaaaatgc agcgtgagaa gttaatgcgg aaaaattccg gataccagcg
     8401 accgtctttg gtttctgctt tttgcagcga gatacagccg accgggcagg ctaccgcaca
     8461 gaggttacag gctacgcaac gctcttcgcc gtccgggtcg cgggtcagaa cgatacgacc
     8521 acgataacgg ggcggcagat agaccggctc ttccgggtac attcgcgttt cgcgtttggc
     8581 gaacgcgtgc aggccgatca tccagatact acgaacctgg gtgccgaaac ctactaacaa
     8641 ttcttttaag gtcatggtct tattgcccct tattgcgcct gccagagaat gacagccgcc
     8701 gttaccagca agttgatcag cgtcagcggc aggcagattt tccagccgaa ggacattacc
     8761 tggtcataac gcggacgcgg taacgacgca cgaatcaaaa tgaacatcat cataaagaac
     8821 gcggttttca gcgcgaacca gatgaatggc ggtaacaacg ggccttgcca gccaccgaag
     8881 aacagcgtca ccatcaatgc agagatggtc acaatcccga tgtactcacc cacgaagaac
     8941 agaccgaact tcataccgga atattcaatg tggtaaccat ccgccagttc ctgctcggct
     9001 tccggctggt caaacgggtg acggtgacac accgccacgc ccgcgatggc aaaggtaata
     9061 aaaccaaaga attgcgggat gacgttccac acatgcgcct ggctgttgac gatgtcggtc
     9121 atgttgaatg aaccggcctg cgccaccacg cccatcaggg aaagcccgag gaacacttcg
     9181 tagctcaggg tctgcgcaga agcacgcatc gcacccagca gcgagtattt gttgttactt
     9241 gaccagcccg caaacagcac cgcgtaaacc gccagacctg ccatcatcag gaagaacaaa
     9301 atcccgatgt tcaggtcggc aaccacccaa cccggactga ctggcacaat tgcaaaggcc
     9361 agcagcagcg aggtaaaggc aatcatcggt gccagggtaa agatgacgcg atccgagaat
     9421 ttcgggatcc agtcttcttt aaagaacatt ttgatcatgt ccgcaaccag ctggagcgaa
     9481 ccgccccagc caacacggtt aggtccataa cggttctgga acagacccag caggcgacgt
     9541 tcgccaaagc tcatgaatgc cccacaggtg acaaccacca gcaggatccc caccgctttg
     9601 aggatggtca gcaggatctc aatcagttcc ggtgatatcc aactcattgt tgtgcctcct
     9661 tgagatcctc aagatgcgcg ccagccagca ccggagcaat gccggacata cccatcggca
     9721 agcccacctg ccctgccgtc agtccttcgg cgatttcaac cggcagcgtg accgtgttgc
     9781 catcgtaact aaaggagacg cgtgtacctg cgttcacacc caacttcgcg gcatccgctg
     9841 ggttgagttt gatgtacggc tgcggcatac ggctctggaa gaccggagca cgctgtgaca
     9901 attcatcgct gccaaacagg tggtagtacg gcgcgatacg ccatttcccg tcctgcggct
     9961 ggaagcgtgc cggtacgctg gtgaagtaat ccagaccgtt ttcgctggtt tcaaacagac
    10021 gcacgcccgg atcgccaaag cgcagtttgc cgcccacttc gtcctggaat ttgttccacg
    10081 cctgcgggga gttccagccc ggcgcccagg caaacggcac ttgcgaacgg tgcgcggtcg
    10141 gctggttgtt accttccatc gagaaggtga acatggtgtc aatatcctgc ggctgacgcg
    10201 gctcatgaac gctgatattg gcgcgcatgg cggtacgacc gctgtaacgg tgcggttcac
    10261 gggccagttt ctgcccacga atacggaatg tcgcatccgg cgcagcatct ttgatacccg
    10321 ccagttccgg gattttcgcc acaacagcgt caattacatg gtcgagctgc gtccagtcca
    10381 cttcacggct cagcaaggtg ctgtgcagcg agtgcagcca gcgccagctt tccagcatga
    10441 cagttttgct gtcgtaataa gccggatcgt aaacctggaa gaaacgttgg gcgcgacctt
    10501 cattattaat taccgtaccg tcgctttcag caaagctggc agcggaaagc accagatggg
    10561 cgttttccat gatcgctgtg cgttgatgat caaccaccat caccagcggc gctttagcca
    10621 gcgcagcatt cacgcgggta gcagaagcgt aacgatgcag atcgttttcc aacaccacca
    10681 ccgcgtcggc gcgtccggtt tccagttcgg ttaacgcttc ttcaagcgaa ccgccaccca
    10741 taatgcccag ccccatgctg ttgacggaac gggcaatcat ggtgatacca acgtcagcgc
    10801 cgcgaccttt cagggctttc gcgacgttag ccgcagcctg aatcacctct atgctaccgg
    10861 cgttcgtccc ggagataatc aacggtttct tcgcacctgc cagtgcctgc acgatgacgt
    10921 cgattttgtt ttgcagctca ggttcgatac cgtcaaccgc tggtgcggag ttatccagcg
    10981 catgggcgat ggcaaaacct aaacgcgcct gatcttcaac cggtgcgcgg taagtccacg
    11041 ccgcgatatc atccagacga gtgtcatcaa cgttggtaac aaacagcgga tgcttcgcac
    11101 gttgaccgat gttgaggatt gccgcaatct gccagtcagc cactttctgt gctgcagcca
    11161 tttcgcgcgc tttacctttc accgcctgac gcactgccag tgcgacgcgc gcgccggtct
    11221 gggtaacgtc ttcgcccagc accagtaccg catcgtaaga ttcgatttcg cgcagagccg
    11281 gagtataaat gccgccttca cgcagcactt tcagcgccag ttgcagacgt tcctgctcac
    11341 cgtgagcgat accggtgtag aagttttctt cgcccaccag ttcacgcagc gcaaagttgc
    11401 tttccacgct ggcacgcgga gaaccaatac cgatcacttt cttcgactga cgcagaatat
    11461 ctgccgcgcc ctgcattgcc tgttcggcgt tgagggtaat gaaatcatcg ccacgacgct
    11521 gtactggctg acgcggacga tccttcaggt tgacgtaacc gtaaccgaaa cgaccacggt
    11581 cgcagaggaa gtagtggttt accgtaccgt tgtaacggtt ttcgatacga cgcagttcgc
    11641 cgtaacgttc accggggctg atgttacagc cgatggaaca ttgctggcag atgctcggcg
    11701 caaactgcat atcccattta cggttgtaac gctcggagtg cgttttgtcg gtaaatacgc
    11761 cggtcgggca aatttcgacc aggttaccgg aaaattcgct ttccagcgtg ccgtcttccg
    11821 ggcgaccgaa gtagacgttg tcgtgcgcgc cataaacgcc cagatctgta ccgtcagcgt
    11881 aatctttgta gtaacgcaca cagcggtagc aggcgatgca gcggttcatt tcgtgagaga
    11941 tgaatggccc caaatcctga ttacggtggg tacgtttggt gaaacggtag cgacggaagc
    12001 tgtgtccggt catcacggtc atatcctgaa gatggcagtt accgccttct tcacataccg
    12061 gacagtcgtg cgggtggttg gtcattaacc actcgaccac actttcacgg aactgtttcg
    12121 cttcttcgtc gtcaatggaa ataaaggtgc catcggaagc cggtgtcata caggacatca
    12181 ccaggcgacc acgcgtgtct tccgcgtttt ggtattgctt caccgcacac tggcggcaag
    12241 caccgacgct tcccagcgcc ggatgccagc aaaagtaagg aatatcaagg cccagagaca
    12301 gacaagcttc cagcaggttg tccgctccgt tgacctcgta ttctttgccg tctacatgaa
    12361 ttgtagccat tagcatgctt ccagttttct cggtcaggga ccgagcgtta atcgaaattc
    12421 ggttaccagc gctctttcag caggttcggc tgaatcccat taatcaaatg ggtattgctg
    12481 aacggctgtt tgattcccgc ctcaaattct tcgcggaaat atttgatggc gctctgtaac
    12541 ggctccactg caccaggtgc gtgggcacag aaagttttac ccgggcctaa gaatcgacac
    12601 agttgctcaa gtgtttcgat atcgcccggc tggccttcgc cacgctccag cgcacgcaga
    12661 attttcacgc tccacggcag accgtcgcgg cacggcgtac accagccgca ggactcacgg
    12721 gcgaaaaact cttccaggtt acgcaccagc gacaccatgt tgatctcatg gtcaaccgcc
    12781 atcgccagcg ccgtacccag acggctgccc gctttaccga tactttcgaa ttccatcggc
    12841 agatcaaggt gcgcttcggt caggaagtca gtccccgcgc cgcctggctg ccaggcttta
    12901 aatttcagac catcacgcat accaccggcg taatcttcga ggatctcgcg tgcggtggtg
    12961 ccgaacggca gttcccacag tcccggattt ttcacccgac cggagaagcc catcagcttg
    13021 gtgccagcat ctttactttt cgagatgttc tgataccact ccacgccgtt agcgaggatc
    13081 gccggaacgt tacacagggt ttcgacgttg ttgacacagg tcggtttgcc ccatacgccg
    13141 gaggttgccg ggaagggggg tttcgagcgt gggttagcac gacgcccttc cagggagttg
    13201 attaacgctg tttcttcccc gcagatgtag cgccctgccc cggtatggac gaacagttcg
    13261 aagtcgaaac ctgttcccat aatgtttttg ccaagcaagc ccgcttcagt ggcttcggca
    13321 atagcgcggc gcagatttac tgccgcttcg atatattcgc cacgcaggaa gatgtagcca
    13381 cggtaagctt tcagcgcaaa cgcggagatg agcatacctt ccaccagcag gtgcggcagt
    13441 tgctccatca acaggcggtc tttataggtg cccggctcca tttcatcggc gttacacagc
    13501 aggtaacgga tgttcatgga ttcgtctttc ggcatcaggc tccacttcag gccagtcgaa
    13561 aagcccgcgc cgccgcgccc tttcagacca gcgtctttta cctgattaac gatttcgtcc
    13621 ggagacagcc cggtcagcgc cttacgcgcc ccttcgtaac cgtttttgct gcggtattcg
    13681 tccagccaca ctggctgttt gtcatcgcgc agacgccagg tcagcggatg cgtttcggga
    13741 gtacggataa tgtttttcat ttataccgct ccagcagttc agggatcgct tccggggtca
    13801 gatgcgcgtg agtgtcctca tcgatcatca tgtttggccc tttatcacag ttccccaggc
    13861 agcaagttgg cagcagcgta aagcggccat caaatgtcgt ttgccctggt ttgatgttca
    13921 gctttttctc gagcgccgcc tgaataccct gatacccgtt gatatgacag accacgctgt
    13981 cgcaataacg gatcacatgg cgaccaaccg gctggcggaa gatctgactg tagaacgttg
    14041 ccacaccttc gacgtcgctt gccggaatac ccagcacatc ggcgatcgcg tggatcgcac
    14101 catccggcac ccagccacgc tgcttctgaa cgattttcag cgcttcaatg gacgccgcac
    14161 gcgggtcttc gtagtggtgc atctcgtgct caatcgcttc acgctctgcc gcactcagct
    14221 caaaagcctc ggtttgtggt tgttgattct cgtgcataat tagcggtcca catctgacat
    14281 aacaaaatcg atactgccca gataaacaat caggtcagac accaagctgc cgcggatcgc
    14341 cgccggaatt tgctgcaaat gcgcataact cggcgtgcgg atacgagtgc gataactcat
    14401 ggtgctaccg tcgctggtca ggtagtaact gttgatcccc ttggtcgcct caatcatctg
    14461 gaaagattca ttggcaggca tcaccggccc ccacgacact tgcaggaagt gggtgatcag
    14521 ggtttcgata tgttgcagcg tgcgctcttt cggcggcggc gtggtcagcg ggtgatccgc
    14581 tttgaacggg ccttccggca tgttgttgag gcactgctca agaatgcgca gactctggcg
    14641 cagctcttcc actttcagca ttacgcgggt gtagcagtca gaaacaccac caccaaccgg
    14701 gatttcaaag tcgaagtttt catagccaga ataaggacgc gccttacgca cgtcgaagtc
    14761 gatcccggta gcacgcaggc ccgcgccagt ggtgccccac tccagcgcct ctttcgcgcc
    14821 ataggcggca acgccctggg tacgaccttt cagaatggta ttttgcagcg ccgctttctc
    14881 gtaagacgcc agacgtttcg gcatccagtc gaggaactca cgcagcagac gatcccagcc
    14941 gcgcggcagg tcgtgcgcta cgccgccaat acggaaccac gccgggtgca tacggaaacc
    15001 cgtgatcgct tccaccagat cgtaaatttt ctgacgatcg gtaaaggcga agaacacggg
    15061 cgtcattgcg ccgacgtcct gaataaaggt cgagatgtac agcaggtggc tgttgatacg
    15121 gaacagttcg gagagcataa cgcgaatgac gttaacgcga tccggcacgg tgatcccggc
    15181 cagtttctct accgccagca cgtaaggcat ttcgttaacg cagccgccga ggtattcaat
    15241 acggtcggta tacggaatgt agctgtgcca ggactggcgc tcgcccattt tctccgcacc
    15301 acggtggtgg tagccgatgt ctggtacgca gtcgacaatc tcttcgccgt cgagttgtaa
    15361 aacgatacgg aaagccccgt gcgccgacgg gtggttcgga ccgaggttga ggaacatgaa
    15421 gtcctcgttt tcggtgccgc gcttcatccc ccactcttcc ggtttgaagg tcagggcttc
    15481 catctccaga tcctgtttgg ctttggtcag ctcaaacggc gagaattcgg tagcgcgcgc
    15541 cgggtaatct ttacgcagcg ggtgaccttt ccaggtttgc ggcatcatga tgcggcgcag
    15601 gttcgggtga ccgtcgaaag taatgccaaa cagatcccag gtttcacgct cataccagtt
    15661 agcgttcggg aacagtttgg tgaaggtcgg tacgtgcagg tcgttttctg ccagcgccac
    15721 cttcagcatg atgtcgcggt tacgatcgat agaaatcaga tggtagaaaa cggaaaaatc
    15781 cgcggcaggt aacccttcgc ggtgtgtgcg cagacgttcg tccatgccgt gtaagtcaaa
    15841 cagcatgacg taaggtttcg gcagtttctt taagaaatcg ccaacttcca gtaattgttc
    15901 acgcttgatc cacacaacgg gaaccccggt gcgagtcgcc tgaacagtaa aggcatccgg
    15961 cccaaaacgg ttgcgcagtt cgccaattac cggatcatca agatgatcgc gggtctgcca
    16021 ggcgggttct tgcgcggtta agtcggtcat attgttcacc attgcaaatg gtccgtggtg
    16081 actgtcggcg cagtaagctt cgcgaaatag atttaggaat aagcgaagaa aaatcccttt
    16141 gccgacaggc gcaaattaaa tctcgtcagg tgtacgcagg ttggttacgg caatgcgttc
    16201 accgcgcttg cgttcgcgct ctgattgcat attggcgcga taaacgccct gatcgccaac
    16261 cacccaggag agcggacgac gttctttgcc gatagattcc tgcaacagca tcagtgcctg
    16321 catgtacgct tcaggacgcg gcgggcagcc cgggatatac acatcaaccg ggatgaattt
    16381 atcgacgccc tgcacaacgg aataaatatc gtacatacca ccagagttgg cacaggcccc
    16441 cattgagata acccattttg gttccagcat ctggtcatac agacgctgaa taaccggtgc
    16501 cattttggta aagcaggttc ctgcaaccac catcaggtca gcctggcgcg gcgaagcacg
    16561 caatacttct gcgccaaaac gcgccacgtc atgcaccgcg gtaaacgaag tcaccatctc
    16621 aacgtaacag caggaaagac cgaagttata cggccaaatt gagtttttac gaccccagtt
    16681 aaccatgtca ttgagcttgc ccataaacac gtttttgtta acttcttgct ccagagggtc
    16741 ggttacgatc tcctgctttt gcagggggta acggtcgttc tcaccgttgg gatctatgcg
    16801 ggtgagcgta taatccatct taatgcctcg cggttagcgt tgacgattag cgatactgtt
    16861 cgtttccggg ttcatacact cgcggcgtga acgcgcgggc gtccagtcca gcgcgccaat
    16921 acgcaccaga taaaccagac ctgccagtaa cacaaaaata aaaattgcag cttccacaaa
    16981 gcctacccag ccgctttcgc ggatagaggt tgaccatgcg aacagataca gcgcttcaac
    17041 gtcgaagata acgaagaaca tggccaccag ataaaacttg gcggacaggc gtaagcgggc
    17101 ggagccgacc gaatcgatac cggattcaaa cggcacgttt ttcgacctcg cgcgtgcgcg
    17161 accgcctaaa aaccaaccgc ctaccagcat caggcaacac aggccaatgg caacgataag
    17221 aaagatagcg aatgcccagt gatgagcgat gacttcagtg gatgttgaca tactcattgc
    17281 ttactcatca aaagtagcgc cagattcact gctcttcacg gcagatggac gccacatcga
    17341 ttcatgggga ggaataaaaa aaaccttaca atcactgtag aaattctttt atacagctaa
    17401 ttgatgtggt cttttactcc tttctataac cttttgtcaa ctttaacaaa agtttcttca
    17461 cattagttta cataatatca acaccattag catttaatgc cctttcaccc cagatccttg
    17521 acgactccag gataattaga tgttgttgaa tcgtgtccgt tgtgaagcaa tggaaaaaat
    17581 acgggtctat tttgacagga atttgtgtcg attcctcccc ccaaaagaga gtattttctt
    17641 gatctgtgac acgcttttgt tattccatag caaaaacgca gcaacaaatt tacgtatttt
    17701 ttaacatcat tgtagcaggt gatttttttc aggcgattat ttgtgcgttc gggacgtgaa
    17761 tctctggtga ttgaaaaata aacagttttg tacgttctgc actatccgag gaaggatttt
    17821 tactaaaaaa aagccgctgg ggtttaaaac acccccagcg gctcgttttt tacactattg
    17881 tctcaggaat tatctatcgt ccgtcgatta ctcgatatcc ctttcaatca acaatgaatc
    17941 atccccttcc ggagcagaca ttgggctgta ttgccacgga ttatggtagc tctccatcgc
    18001 ctgatagatc acctgggcca gctcattatt actggacggg tcatagcaca gcaaatattc
    18061 ggtatcaggt agcggcggta agccatctac tccactcagc acacgcaggt ccgggctcat
    18121 catctcaacc ggccttgccg tcacgccaag accggctttc actgccgcac gaacggccgg
    18181 aagcgtcgag gcgacataag ccagtcgcca tggaatatct gctttattaa gcgtcgccag
    18241 caccatatcg cgaaacggac ttggatcatc cagcaataca agagggatcg gctcgccttt
    18301 ttgcaatacg tattccgctg cgcagtacca gtgtgttggc gaggtacgca ggttcaatgc
    18361 cttaaacgct gaaggacgat gggtggttac catcaaatcc acttcctggg attccagcat
    18421 ttctgccata taggcattac gtttaacgcg gacatccagc gctaatttcg gataaaccga
    18481 actcacgcga tttaacagga aaggtaagat cgtatcggca gattcatctg aagcaccgat
    18541 agttaacacg ccctgaagat tactgaacat taatgatgag caggcctcat cattaaaacg
    18601 caggattttc ctggcgtaac caagaagttg aatgccatgt tcagttaaca gtttgttgcg
    18661 accgtgacga gcgaacagtt ctttcccaac gagttgttcc agacgctgca tttgctgact
    18721 tacggcggac tgagtacgac acacagcggc agctgcggca gcaaaagtgt tcagatcggc
    18781 aacagcaaca aatgttctca gcagatcgag gtcgaggtta attatcggac gatttgcact
    18841 tatcatatat tatcacttac tggcggctca tactgagctg gttaatgctg tgcacacaca
    18901 aacaagcaat tccatttgta atgtgcctcc ctggcagttt catccggaac ccggacgaaa
    18961 gtaaaaatgc atatgagttg cactaaaaaa gcgactcaca ttgttccgtt ataatgcctg
    19021 aagtagatca cagaatatat cttcagggat cgcatatcta ttaagttact cactcttttc
    19081 tatttatgac atgcgcgtgt ttgtataaat gtaaatgtga gtccttgttc cactctcgtg
    19141 cagcatcgct ggtcatacgc gaacacgtac aaacagcaat ggtgaagcgg catcaagagc
    19201 agggatccgt catttatgcg agcattttac cccaaaaacc ttttatttat aagggtcatt
    19261 gcgaattatc tgatgcaaag ttatgttatg ttatgttatg ttaggcaaag taatcttcta
    19321 ttattaataa gcacatcaaa acctttttga atattaaata ataattaatt agcatcatcc
    19381 tcattcatta attccgctta acaatagttt cacaccttac ccccattgcg acacctcgga
    19441 aatcatcact ctgttaaact ctccgtaatg cggaaatatc atcatcgcgt ttcattagtg
    19501 aattcttaaa tgaggcattt tcacacaatt atcttacaga taaaaaaacc atacttgcaa
    19561 ttaagaatta gaacaattca ctatataaca ttacatgtaa agcatataca cctaattatt
    19621 ttgtcattat taagttatta acagcacaat cgagccttcc cctctggcaa aatcttattc
    19681 tgcagacctt caaaacaccg tcctggggga gtacattgtt ctaagctgac ttccacggca
    19741 gggagtggcg ataacagcaa aaaaggtcaa gattcatgtc ccccattgaa aaatccagca
    19801 aattagagaa tgtctgttat gacatccgtg gtccggtgct gaaagaagca aaacgcctgg
    19861 aagaagaagg taacaaggta ctgaaactga acatcggcaa cccagcccca ttcggttttg
    19921 acgcgccaga tgaaatcctc gttgacgtga tacgcaacct gcctaccgct caagggtatt
    19981 gcgattccaa agggctttac tccgcgcgta aagccatcat gcagcactac caggctcgtg
    20041 gcatgcgtga tgttaccgtg gaagatattt acatcggcaa tggtgtatcg gagcttatcg
    20101 ttcaggcaat gcaggcattg ctgaacagcg gggacgaaat gttggttcct gcaccagatt
    20161 acccactctg gaccgcggcg gtttcgcttt ccagcggtaa agcggtgcat tatctttgcg
    20221 atgaatcctc tgactggttc ccggacctcg atgatattcg cgctaaaatt acgcctcgta
    20281 cgcgtgggat tgttattatc aacccaaata acccaaccgg cgcggtatat tccaaagagc
    20341 ttttaatgga gattgtggag attgcacgtc agcataatct cattatcttc gccgatgaaa
    20401 tttatgacaa aattctctac gacgatgctg agcatcactc aattgcgccg ctggcacctg
    20461 acctgctgac cattaccttt aacggactgt cgaaaacgta ccgcgttgca ggcttccgtc
    20521 aggggtggat ggtgttgaac gggccgaaaa aacacgccaa aggctacatc gaaggtctgg
    20581 aaatgctggc ttcaatgcgc ctgtgtgcta acgttcctgc gcaacacgcc attcagaccg
    20641 cgctgggtgg ttatcagagc atcagtgaat ttattacccc tggcggtcgt ctttatgagc
    20701 agcgtaaccg cgcgtgggaa ctgatcaacg atattccggg cgtttcctgc gtgaaacctc
    20761 gtggtgcgct gtatatgttc ccgaaaatcg acgccaaacg ctttaacatt catgacgatc
    20821 agaaaatggt gctggatttc ctgttgcagg aaaaagttct gttggtgcaa gggacggcat
    20881 tcaactggcc gtggccggat cacttccgca ttgtcacgct accgcgtgtc gatgatatcg
    20941 agctgtcttt gagcaagttc gcgcgtttcc tttctggtta tcatcagctg taatcttaat
    21001 ttcactgccg gagattgcat ccggcagtgt tatcccgcca caatgacctg atgatgtcat
    21061 catacgtaag gtcactatga aacagagcca tttttttgcc catctctccc gcctgaaact
    21121 cattaaccgc tggccgctaa tgcgcaacgt gcggacggaa aatgtgtccg aacacagttt
    21181 gcaggtagcg atggtcgccc atgcgctggc agctatcaaa aatcggaaat ttggcggtaa
    21241 tgtcaacgcc gaacgtatcg ctttactggc gatgtaccac gatgcctcag aagtgctcac
    21301 cggcgatctc cctactccgg tgaaatactt caatgcgcaa atcgctcagg agtacaaggc
    21361 tattgaaaaa atcgctcagc agaaactggt cgatatggtt ccggaagaac tgcgggatat
    21421 ctttgcgccg ttaattgacg agcatgcata tagcgatgaa gaaaaatcgc tggtgaaaca
    21481 ggcagatgca ctgtgtgcat atctgaaatg tctggaagaa ctcgcggccg gaaataatga
    21541 attcttgctg gcaaaaacgc gactggaagc gacgcttgaa gcgcgtcgca gccaggagat
    21601 ggactacttc atggaagtat ttgttcccag cttccatctt tcgctcgatg agattagcca
    21661 ggattcaccg ctgtaagcag ccggagtctg cgtcgcatca ggcaataagc gccggatgcg
    21721 acatcaggct cttgtcaaaa cggaaacagc atcgggatca tcaccacaca aaccgccatc
    21781 acgataatgg tgaacggtac ccccaacttc acaaagtcac tgaagctgta atttcccgga
    21841 cctaaaacca gggtgttaac aggtgaagac accggtgtca taaaggcggc ggatgctgcc
    21901 atcgccacga ccatcgcaaa tggataaggc gacactccca tcgttttggc agcagccagc
    21961 gcaatcggag ccatcaacac cgccgtcgcg gtattagaga taaacagccc aataaccgcc
    22021 gacaagacaa acaaacaccc cagcatcata tgtggcccat aaccgccgcc aatatccatc
    22081 agccctttca ccgccagcgc gacacctccc gttttctgta atgccacagc aaacggcatc
    22141 atcccaacga tcaaaataat gctcggccag tgaatggatt tataggcgct ttcagcatct
    22201 atacagcgga atttccccat cagcaggcag gcgatgatag cggcaacagg attaggaatt
    22261 tcatttgtca gcattaacgc caccattagc accagacaga aaatggcatg gggtgcctgg
    22321 ctgtgcgcgg gcgatgcttc actcacctct tccggtaagt tcagcgctac gaagtcgcgg
    22381 ccctgtttgg ccagcatacc gatcagtttc cagttaccca caaccaggat gatatcgccc
    22441 agcagcagag gctcatccgc cagcgaacct tccagcgcca cgccattgcg cttgagcccc
    22501 accacattca gtccgtagcg ggtacgaaaa ccaatttcgc gcaccgattt accaatcagt
    22561 tctgactcag gaattaatga aatctctgcc atgcccacat caagggcctg gtcagaaaaa
    22621 tactcgccgc gcagtaccat cggctccagc aattgctcac tacaaaattg ccggagatcg
    22681 acatcagccg cagacatatc aataagcaaa acgtcacgcg cgcgaaattc agaaacccca
    22741 ttaacgttca cgataacgcg acgaaaacgc cgccagcgtt caacaccgat gacgttagcg
    22801 ccataacgct cacgtaattt gagatcatcc agccgttgac ctatcattgg cgatccgggg
    22861 cgaatagcca gacgtcgcgc acgcccggtc agtcgatatt cacggataag atcgcgaaag
    22921 gttcgacgcg tccagccttc gcgctgcggg gtctgggtat cccctttcag catgaaacgc
    22981 atcactaaca tatacaaaat acccagcacc agcacgacca ggccaatagg tgttacgcta
    23041 aagaaactga agccgtgata gccttcacgc agcaattcac tgttgactac caggttcggc
    23101 ggcgtcgcca ccagcgtcat catgccgcta atcagcccgg caaaacttaa cggcatcatc
    23161 agacgtgacg gcgaagtttg catacgcatg gcaacgctta acaccacggg aataaagata
    23221 gcgacaacgc tggttgaact cataaacgcg ccaagccccg cgacggtcag catcaacaaa
    23281 accaacattt tgatttcact gttgcccgcc actttgacca gccatgttcc cattacggtg
    23341 gcaacaccgg tacggaccaa accatcgcca ataataaaca aggcggcaat caggacaacg
    23401 ttaggatcag aaaagccgga aaatacttcg gggaccgtca gcgttccgct taatgcaaac
    23461 gcgacaataa caaacaaagc gaccgcatcc atacgcactc tgcccgtcgc aaacaagacg
    23521 atggcaacgg ccagtaatga aagaacccaa atcaattcac cgttcacaac ttatccttgt
    23581 taattgaggg ggatgacttg attctgccat aaaaaagccc cgacgagacg gggctaaatc
    23641 atgatcaggt gtttcactga ataataacat cgccatttgg ctgtttggtc acagtaattt
    23701 gctccagact gtggaggacc aaatcgacct cattcaggcg cggggtatct gccggagcgt
    23761 taaccgcaat gacatggcaa cccgccgcta ggccagaaag cacgccagcg ggagcatctt
    23821 ccaccaccac acactcctgc ggcgcaagcc ccagcagctg cgcacctaac agatacgcat
    23881 caggttctgg ttttccgcgc ttcactcgct cagcggttac aaacacctct ggtgcgggaa
    23941 gccccgctat tttatggcgc gctcgcgcta ccggcatgga gccagaagtc acaatggccc
    24001 acggaatacc tgctttattc aaatgattga gtaaggcgat tgccccagga agcgcggtaa
    24061 taccttcggt ttccgtggcc tcgatttgct ccaaacgcgt aaactcggcg gcaatatcag
    24121 cctcggattt gcccgccata aaatggcgca aagaggtgat cgcctgttta ccgtgaatga
    24181 aagccagcac ctcttccggc gctaacccat gacgtctggc ccagttgctc cacgcccgtt
    24241 ctaccgcagg cagggaatcc accagcgttc catcaagatc aaacagaaaa cctttgcacc
    24301 gcacgcgggc ctcctcaggc gttgataatt tgattaattt cgttggcgct caaatggtac
    24361 tgacgcgggc aggcatgcca cacattaagc atgcgctgat atttttccca cattggcgtc
    24421 tgggcgttaa aaccatgagt cccagcgtca aaatgggtat agcgcccttc cacattaacc
    24481 ataaagcgga cataaccgag gtaacgtgct tcagtggcgg cgtcaaagcc gaggaaggtg
    24541 acacgacgtt catcgatgga ttgctggtcc tgcaaattag accaggaaac atgcaacgca
    24601 tgatacatct ccataatgtc gatgatagtg cggcaggttt cttctttcag ctcgccaaac
    24661 tcgcgatcca attcacgcat ctgtaatccg taaccacgct caataattgt ttgcaggcga
    24721 cggtaacgtt cagcatttgc cggatcgagc atagtcatca tcttgtactg attagacaaa
    24781 ataagacgtt gcgcgttggt catttccatt gttgactcct gtatcactct actacggtga
    24841 aaaaaaagaa ggctgagtat gccttctttt atatgcgtaa tcaggggtca attacaaatc
    24901 atcaaggaaa gttttatcca gttgtttgaa ggcgcgctta agcgtgtcag ctaatgcctg
    24961 gtaatcaggc ttgccttcaa cgggtgccaa cacctgtcca gactcctgca atttaccgcg
    25021 aacttcataa aaccagttaa ggattgcagg gggtaatggc gttacagaac gcttgcccag
    25081 ccaccacaat ccctgcatgg gtaaacttaa ggcgaacagt gcagtggcaa ctgccggccc
    25141 aagcagaccg cccagggcaa tctgccagca gagagtaaat acggcgatcg gcggcataaa
    25201 acggatcgca taacgcgtca tcttgataac gcgattttcg acaaagaccg gggcaaggcg
    25261 tttttccagt ggccacgtct ttgagtaatg ctgtccgcgg cgaaacaagc taaaaaaatt
    25321 aacagaacga ttatccggcg ttgacatgct tcacctcaac ttcacatata aagattcaaa
    25381 aatttgtgca aattcacaac tcagcgggac aacgttcaaa acattttgtc ttccataccc
    25441 actatcaggt atcctttagc agcctgaagg cctaaatagt acatattcat tgagtcgtca
    25501 aattcatata cattatgcca ttggctgaaa attacgcaaa atggcataga ctcaagatat
    25561 ttcttccatc atgcaaaaaa aatttgcagt gcatgatgtt aatcataaat gtcggtgtca
    25621 tcatgcgcta cgctctatgg ctccctgacg tttttttagc cacgtatcaa ttataggtac
    25681 ttccatgtcg agtaagttag tactggttct gaactgcggt agttcttcac tgaaatttgc
    25741 catcatcgat gcagtaaatg gtgaagagta cctttctggt ttagccgaat gtttccacct
    25801 gcccgaagca cgtatcaaat ggaaaatgga cggcaataaa caggaagcgg ctttaggtgc
    25861 aggcgccgct cacagcgaag cgctcaactt tatcgttaat actattctgg cacaaaaacc
    25921 agaactgtct gcgcagctga ctgctatcgg tcaccgtatc gtacacggcg gcgaaaagta
    25981 taccagctcc gtagtgatcg atgagtctgt tattcagggt atcaaagatg cagcttcttt
    26041 tgcaccgctg cacaacccgg ctcacctgat cggtatcgaa gaagctctga aatctttccc
    26101 acagctgaaa gacaaaaacg ttgctgtatt tgacaccgcg ttccaccaga ctatgccgga
    26161 agagtcttac ctctacgccc tgccgtacaa cctgtacaaa gagcacggca tccgtcgtta
    26221 cggcgcgcac ggcaccagcc acttctatgt aacccaggaa gcggcaaaaa tgctgaacaa
    26281 accggtagaa gaactgaaca tcatcacctg ccacctgggc aacggtggtt ccgtttctgc
    26341 tatccgcaac ggtaaatgcg ttgacacctc tatgggtctg accccgctgg aaggtctggt
    26401 catgggtacc cgttctggtg atatcgatcc ggcgatcatc ttccacctgc acgacaccct
    26461 gggcatgagc gttgacgcaa tcaacaaact gctgaccaaa gagtctggcc tgctgggtct
    26521 gaccgaagtg accagcgact gccgctatgt tgaagacaac tacgcgacga aagaagacgc
    26581 gaagcgcgca atggacgttt actgccaccg cctggcgaaa tacatcggtg cctacactgc
    26641 gctgatggat ggtcgtctgg acgctgttgt attcactggt ggtatcggtg aaaatgccgc
    26701 gatggttcgt gaactgtctc tgggcaaact gggcgtgctg ggctttgaag ttgatcatga
    26761 acgcaacctg gctgcacgtt tcggcaaatc tggtttcatc aacaaagaag gtacccgtcc
    26821 tgcggtggtt atcccaacca acgaagaact ggttatcgcg caagacgcga gccgcctgac
    26881 tgcctgattt cacaccgcca gctcagctgg cggtgctgtt ttgtaacccg ccaaatcggc
    26941 ggtaacgaaa gaggataaac cgtgtcccgt attattatgc tgatccctac cggaaccagc
    27001 gtcggtctga ccagcgtcag ccttggcgtg atccgtgcaa tggaacgcaa aggcgttcgt
    27061 ctgagcgttt tcaaacctat cgctcagccg cgtaccggtg gcgatgcgcc cgatcagact
    27121 acgactatcg tgcgtgcgaa ctcttccacc acgacggccg ctgaaccgct gaaaatgagc
    27181 tacgttgaag gtctgctttc cagcaatcag aaagatgtgc tgatggaaga gatcgtcgca
    27241 aactaccacg ctaacaccaa agacgctgaa gtcgttctgg ttgaaggtct ggtcccgaca
    27301 cgtaagcacc agtttgccca gtctctgaac tacgaaatcg ctaaaacgct gaatgcggaa
    27361 atcgtcttcg ttatgtctca gggcactgac accccggaac agctgaaaga gcgtatcgaa
    27421 ctgacccgca acagcttcgg tggtgccaaa aacaccaaca tcaccggcgt tatcgttaac
    27481 aaactgaacg caccggttga tgaacagggt cgtactcgcc cggatctgtc cgagattttc
    27541 gacgactctt ccaaagctaa agtaaacaat gttgatccgg cgaagctgca agaatccagc
    27601 ccgctgccgg ttctcggcgc tgtgccgtgg agctttgacc tgatcgcgac tcgtgcgatc
    27661 gatatggctc gccacctgaa tgcgaccatc atcaacgaag gcgacatcaa tactcgccgc
    27721 gttaaatccg tcactttctg cgcacgcagc attccgcaca tgctggagca cttccgtgcc
    27781 ggttctctgc tggtgacttc cgcagaccgt cctgacgtgc tggtggccgc ttgcctggca
    27841 gccatgaacg gcgtagaaat cggtgccctg ctgctgactg gcggctacga aatggacgcg
    27901 cgcatttcta aactgtgcga acgtgctttc gctaccggcc tgccggtatt tatggtgaac
    27961 accaacacct ggcagacctc tctgagcctg cagagcttca acctggaagt tccggttgac
    28021 gatcacgaac gtatcgagaa agttcaggaa tacgttgcta actacatcaa cgctgactgg
    28081 atcgaatctc tgactgccac ttctgagcgc agccgtcgtc tgtctccgcc agcgttccgt
    28141 tatcagctga ctgaactggc gcgcaaagcg ggcaaacgta tcgttctgcc ggaaggtgac
    28201 gaaccgcgta ccgttaaagc agccgctatc tgtgctgaac gtggtatcgc aacttgcgta
    28261 ctgctgggta atccggcaga gatcaaccgt gttgcagcgt ctcagggtgt agaactaggt
    28321 gcaggcattg aaatcgttga tccagaagtg gttcgcgaaa gctatgttgg tcgtctggtc
    28381 gagctgcgta agaacaaagg catgaccgaa accgttgccc gcgaacagct ggaagacaac
    28441 gtggtgctcg gtacgctgat gctggaacag gatgaagttg atggtctggt ttccggtgct
    28501 gttcacacta ccgcaaacac catccgtccg ccgctgcagc tgatcaaaac tgcaccgggc
    28561 agctccctgg tatcttccgt gttcttcatg ctgctgccgg aacaggttta cgtttacggt
    28621 gactgtgcga tcaacccgga tccgaccgct gaacagctgg cagaaatcgc gattcagtcc
    28681 gctgattccg ctgcggcctt cggtatcgaa ccgcgcgttg ctatgctctc ctactccacc
    28741 ggtacttctg gtgcaggtag cgacgtagaa aaagttcgcg aagcaactcg tctggcgcag
    28801 gaaaaacgtc ctgacctgat gatcgacggt ccgctgcagt acgacgctgc ggtaatggct
    28861 gacgttgcga aatccaaagc gccgaactct ccggttgcag gtcgcgctac cgtgttcatc
    28921 ttcccggatc tgaacaccgg taacaccacc tacaaagcgg tacagcgttc tgccgacctg
    28981 atctccatcg ggccgatgct gcagggtatg cgcaaaccgg ttaacgacct gtcccgtggc
    29041 gcactggttg acgatatcgt ctacaccatc gcgctgactg cgattcagtc tgcacagcag
    29101 cagtaatctc gtcatcatcc acagctttgc gctgcggata tctgaaccgg aaataatcac
    29161 tatttccggt tttttattct cttaatttgc attaatcctt tctgattatc ttgcttaact
    29221 gcgctgcatc aatgaattgc gccatctcac tttgcatact taccactttg ttttgtgcaa
    29281 gggaatattt gcgctatgtc cgcaatcact gaatccaaac caacaagaag atgggcaatg
    29341 cccgatacgt tggtgattat cttttttgtt gctattttaa ccagtcttgc cacctgggta
    29401 gttccggtgg ggatgtttga cagtcaggaa gtgcagtatc aggttgatgg tcaaacaaaa
    29461 acacgcaaag tcgtagatcc acactcattt cgcattctga ctaacgaagc aggcgaacct
    29521 gagtatcacc gcgtacagct gttcacgacg ggcgatgaac gcccgggcct gatgaacttc
    29581 ccgtttgaag ggttaacctc aggatcgaaa tacgggacag ccgttggcat catcatgttt
    29641 atgctggtga ttggcggcgc gtttggcatt gtgatgcgta caggaaccat tgataacggt
    29701 atcctggcgc ttattcgcca tacccgcggg aatgaaattc tctttattcc tgcgctgttt
    29761 attctgtttt cacttggcgg cgcggtattt ggtatgggag aagaggccgt cgcctttgcc
    29821 attatcatcg caccgctaat ggtccggctg ggctatgaca gtattaccac cgtcctggtg
    29881 acttatattg ccacgcaaat cggttttgcc agttcgtgga tgaacccgtt ttgtgtggtt
    29941 gttgctcagg ggattgccgg cgttccggtg ctttctggct ccgggttgcg catcgtggtg
    30001 tgggttatcg ccactctgat tggcctgatc tttaccatgg tgtacgcctc acgagtgaaa
    30061 aagaatcctc ttctgtcacg cgtgcatgag tccgaccgct tctttcgtga aaagcaggcg
    30121 gatgttgaac aacgtccgtt tacctttggt gactggctgg tattgattgt cctgacagcc
    30181 gtaatggtct gggtgatttg gggcgtgatc gttaatgcct ggtttattcc agaaattgcc
    30241 agccagttct tcaccatggg tctggtgatt ggcatcatcg gcgttgtttt ccgccttaac
    30301 ggcatgacgg ttaataccat ggcttcatcc tttaccgaag gggcgcgaat gatgatcgcc
    30361 cctgccctgc tggtgggttt cgccaaaggg attttgctgc tggttggtaa tggtgaagca
    30421 ggtaatgcca gcgtgttaaa tactatcctc aacagcattg ccaatgccat tagcggtctg
    30481 gacaacgcgg tcgcggcctg gtttatgttg ctcttccagg ctgtatttaa tttcttcgtg
    30541 acgtccggtt ctggtcaggc ggcattaact atgccgttac tggcaccgct tggcgatctg
    30601 gtcggtgtta accgtcaggt taccgtgctg gcgttccagt ttggtgatgg cttcagccac
    30661 atcatttacc caacctcagc ttcgttaatg gcaacgctcg gtgtgtgccg ggtggacttc
    30721 cgtaactggc tgaaggtggg cgcgaccctg cttggactgc tgtttattat gtccagcgtc
    30781 gtagtgatcg gcgctcagtt gatgggctac cactaaaaat gttaagagcc gcaattgcgg
    30841 ctcttttttc attctgccgt ttcagtctct acggcttcat ttttggcatt gcgcttcatc
    30901 cacaacgcca gcgctttcag cgagtctgga gtgaactcat cgcagcgtgc ggtgatttct
    30961 tccggcgtca gccagcaaac ttcactgact tcatcttcct gtagggcgaa gggaccgtga
    31021 gagacgcaac tgaacaacgc gccccagaca cggcaatttt tatcttcgaa atagaactgc
    31081 ccgtgctcgg caaaggggac accggcaatg cccaactctt cttccgcttc gcgacgcgcg
    31141 gattccagca gttgctcatc ggcctgaact acaccgcctg cggtcgcatc taacatgccg
    31201 ggtaaaaagt cttttgtctc ggtacgacgc tggaccagaa ttttgcccat gccatcatgc
    31261 acgacgatgt aagttgcacg atgacgcaga cactgtgccc gcatttgttc ccggctggct
    31321 tgtgcaatga cttcgttctc ttcattgaca atatccaccc attcagtact tgccaaacga
    31381 cgctgttcca ttatcaggaa accttctttt tctggcgctc ttacggcgca ttttgagttg
    31441 tgggtaaatt acggattaat cgcgacctgc gcaatgatac tttgatcatt gagtgcgatt
    31501 acactaagta cgtcattatc cagcatgcca taactcgccg gattaccgcc tttcggaata
    31561 ctcaccgagc cggggttgaa gtggaaaatc tccccccgct gttctgccac tggcagatgg
    31621 gtatgaccgt acaccagcac atcgttctgg tttaaagcag gtagattttc cgggccaaaa
    31681 agatgaccat gcgtcaaaaa cagacgttgt ttttccagta atacctgttg ccacggcgcg
    31741 gttatcggga aatgcagcat catttgatcc acttcgctgt cgcagttgcc acgcacagcg
    31801 ataaccttat gtgccacttc attaagccgt tcagcaactt tggctggcgc gtaaccctcc
    31861 ggtaaagcat tacgcgggcc gtgattcaac acgtcgccaa ggatcaccag ccactgggca
    31921 ccgctttgga caaacaactc cacaacacgt tccgtcgccg gtaacgaccc atgaatgtcc
    31981 gatgcaaaca tcagtttcat cactcactcc tcgtcgaaaa aacgtaagct atgatactgg
    32041 attctgtcgt tgctatcagc cagattgctt cgccgaaagt gcaataaaac gctggacaga
    32101 cgctcgctgc cactggaatg tcgcataatc caccaggcgt tctggcacct catcgccatg
    32161 tagtaccagg cggttaatca ttagcgccag atcagtatca gcaatgcacc attcaccaaa
    32221 taaattcggc tgacccagtg ctaacaaatg ttctgccatc gcgaacagtt tctctgcact
    32281 ggcttttccc tcggccgtta gtggcgcttt tttcgccccc gcaaagacaa catccgtcgg
    32341 acgctcttcg cggatgggca tcagatcgct gcgcagccag gcctgaatct gtcgtgcacg
    32401 cgcacgattt tctaaatcaa gcggataaat acgttcccag gtcggtggcg caaatcgatc
    32461 ttccagatac tccgcaatgg cagaagattc actcaactca aaatcatcga tttgtaataa
    32521 cggcacacgg cgtgtctgac cgtaaccttg ccacgtcggc tgcaaatgtt caccgctgtc
    32581 gaggtcgatg gtcttgatat gaaacgacag gcctttttcc tgcaacgcca cccaggcgga
    32641 taacacataa ggggagaaaa agtgggcatc tgaccaaagc gtgatagcgg gtttactcat
    32701 tatgtcctcg gcaataaagg gggctggcta tcaaactata gagccttttt gtcactgtca
    32761 cgtgacgaat actcacgtaa gtcgccgcgc tttctatact tgtggtgttc agatgattaa
    32821 acaaccggag ctgcaatgat cgatctctat ttcgccccga cacccaatgg tcacaaaatt
    32881 acgctgtttc tcgaagaagc agagcttgat tatcgcttga ttaaggtaga cctggggaaa
    32941 ggcggtcagt ttcgtccgga atttttgcgc atttcgccca acaacaaaat tccggcaatt
    33001 gttgatcatt ctcctgccga tggcggcgaa ccgttaagcc tctttgagtc tggtgccatt
    33061 ttgttgtatc tggctgagaa aacaggactc tttttgagtc atgaaacgcg tgagcgcgcc
    33121 gccacattac agtggttatt ctggcaggta ggcggactgg ggccgatgct tgggcaaaat
    33181 catcatttta atcacgcagc cccccaaacc attccttacg ctattgaacg ttatcaggtt
    33241 gaaactcagc gtctttacca tgtactaaac aagcggctgg aaaactcgcc ctggctggga
    33301 ggcgagaact acagcattgc ggatattgcc tgctggccgt gggttaatgc ctggactcgc
    33361 ctgcgaattg acctcgcaat gtatccggca gtcaagaact ggcatgagcg gatccgttcg
    33421 cgccctgcca ccgggcaggc actgctaaaa gcacaactcg gtgatgagcg ttcggatagt
    33481 taacagaaac aggttctcgt gtattatttc atcctaagta aaacaacgga gaacctgcaa
    33541 tggcacaacc tgccgctatt attcgtataa agaaccttcg tttgcgtacg tttatcggaa
    33601 ttaaggaaga agaaattaac aaccgtcagg atattgttat caatgtgacg atccactacc
    33661 ccgccgataa agcgcgcact agcgaagata tcaacgatgc gctgaattat cgcaccgtaa
    33721 cgaaaaacat tattcagcat gtagagaata accgtttctc tttgctggaa aaattaactc
    33781 aggatgtgct cgatatcgta cgtgaacatc actgggtgac gtatgctgaa gtggagatcg
    33841 ataaactgca cgcgctgcgc tacgccgatt cggtatccat gaccttaagc tggcagcgtt
    33901 aatcgccata tcgggaggct gcatgaacat agtgatcacc ggagggacgg gattaattgg
    33961 tcgccatttg attccgcgtt tgctggagct gggccatcaa attacggtag tgacgcgtaa
    34021 cccgcagaaa gccagttccg ttctcggccc tcgggtgaca ctatggcaag ggcttgccga
    34081 tcaaagcaac ctcaacggcg ttgatgcggt aatcaacctg gccggagaac cgattgctga
    34141 taaacgctgg actcacaagc aaaaagagcg tctctgccaa agccactgga atatcacgca
    34201 aaaactggtc gatttgatta atgccagcga cacgccgccg tcggtactca tttccggctc
    34261 ggcaacgggc tattatggcg acttaggtga agtggtggtt accgaagagg aaccgccgca
    34321 taacgaattt actcataaac tctgcgcccg ctgggaagag attgcctgcc gggcgcaaag
    34381 tgacaaaacg cgagtgtgcc tgctgcgaac cggcgtggta ctggcaccgg acggcggtat
    34441 tctcggtaaa atgctgccac cgtttcgtct tggcctgggc gggccgattg gttccggtcg
    34501 gcagtatctg gcctggattc atattgatga tatggtcaac ggcattctct ggctgctgga
    34561 taacgagctg cgcgggccat ttaatatggt ttcgccctac ccggtacgca atgaacaatt
    34621 tgcccatgcg ctcggtcatg cgctgcatcg cccggctatt ttgcgcgtcc ccacaaccgc
    34681 cattcggctg ttaatgggcg aatcttcagt actggtatta ggtggacaac gcgcgctgcc
    34741 taaacggctg gaagaagcgg gttttgcgtt tcgctggtac gatttagaag aggcgctggc
    34801 ggatgtcgtt cgctgatgtg gtttacagca aacatccgcc agttaactcc ctgtgttaca
    34861 ggattagtgg ctttgcgcga taagatcgtc tggtgaaagt cgggtcacca tcataactaa
    34921 ctctctgtct aaacctctat ccagcatctc ctgagcaata cgcagggctt cttcgtgttt
    34981 gccctgcatt gcgccttctt cacgtaatct gtcagcaatg gtcatcagtt tctccttttc
    35041 ttgtggtgcg cgttctgtta tctcaccaat aaatgcacgg aaacgctggg catcccctgt
    35101 ttgtaatacg taattaaaca gggcttttag ctgtctgtca ttagtgttcc ctgtaactag
    35161 cagcgaaaca atttggtcga ctaatcccaa cagatcgcgc tgacgaatat gtttctgaat
    35221 taactccaac agcgccattt tgcggtgttg cataatctcg tcatccggca ccactgtaat
    35281 atccaccaac ggaaacgctg atgaatatat tttgcgggct atagcaggct cggcaaattc
    35341 atcaagccag cagagtgaat aaggataagg acttctgcaa ccatgataaa acagcatcgg
    35401 gatcaccaat ggaagctctt tatagcccgc atcaagatgg ttttgcattg ccgcaatgga
    35461 ataacgcatc atgcgaaaag ccattaattc ttccggctta ctttggtgct ctatcactac
    35521 ataaatataa cccactccct cctgcgtttt cacagaccac aagaggtcgg aataatattg
    35581 ccgcaggtct tcatcaataa aactgtttgg ttctagttta agcgtcgtta aatcacacag
    35641 tttgcgcagc ggcgcgggaa gatgaatatt aataaaattc cgcgcggtgt ctggatggcg
    35701 taaaaaagat ttaaataccg catcatgcgg cgtggaagtt gtcgatattg tcatggcgtt
    35761 ccgtcacccc tcaaatttaa gatgacgcga caataaccac gattaacacc caatgcagca
    35821 ggcagttttt atctttggac agcgaattca gaagtatttt tagcggactg caacttttac
    35881 atttcagcgg aagctgactc gcaaattccc cttgaaaata ctccggcagc gttagccacc
    35941 ggagaggaaa ttatttcagc gatcccttaa ggaactgttg cagacgaggg ctttgcgggt
    36001 tgccaaataa ctgctccggc gcgccctctt cttctatttt cccctgatgg aggaaaatga
    36061 cgtgagtaga aacatggcga gcaaagccca tttcgtgagt cactaccacc atggttttcc
    36121 cctcttctgc cagttgctgc ataatacgta acacttcgcc taccagttca ggatcgagcg
    36181 ccgaggtagg ttcatcaaac agtaaaactt ctggttccat cgccagcgcc cgcgcgatag
    36241 aaacacgctg ttgctgaccg ccggaaagat gcaccggata tttcccctgc gcacgttcgt
    36301 ctatcccgac tttcgccaga tacttcaccg cccgctcgcg cgcttcctgc ttgctcaggc
    36361 ccaacacctg aatcggcgct tccatgacgt tttccaacac cgtcatatgg ctccagagat
    36421 tgaagtgctg gaataccatc gtcaggcgtg tgcgcagtaa gcgcagttga tttttatcgg
    36481 cgactttgag ttgaccgtct ttgtcgcgca ccagattgat cgtctggcca ttgaccacga
    36541 tcgatccttc actcggtttt tcgaggaagt taatgcagcg cagaaaggta cttttccccg
    36601 atcccgacga tccgatgatg cttattacat ctccggcatt cgcttgcagt gagacccctt
    36661 tcagcacttc gtgttcgcca tagcgtttgt gcaaatcgat aacgtttaat ttattctcgg
    36721 acatcgtatt ctcagtgcgt tgaagaaggt ttcacatgct gcaaccagcg tttttccgct
    36781 ctgcgaaaga ggctgatcag gacataagag atgattaaat agagcaccgc ggcaatgccg
    36841 aaggcggtaa aaggttgata cgtggcggcg ttaatatcgc gggctatttt cagcaaatcc
    36901 ggcacagtgg ccgtaaacgc cagcgcagta gagtgcagca tcaggatcac ttcgttgctg
    36961 tatgccggta acgcaatacg cagcgcagaa ggcaaaataa tgcagcgata cattttaaag
    37021 gtcgagaagc cataggctct ggcggcttca atttccccat gcggaaccga acggattgcc
    37081 ccagcaaaaa tctcggtagt gtaagcgcag gtgttaagcg tcagcgccag cacggtacag
    37141 ttcaggccac tgcggaagaa agcgttaagg aattcggttc ccttaacaat ctcaagcgtg
    37201 tacatgccgg aatagaacac cagcaactga acatacagcg gcgtaccgcg aaaaatatag
    37261 gtaaataacc agattggaaa ctggatgtat ttattactgg agacacgacc aatcgccaga
    37321 aacagcgcca ggactccgcc tatcactacc gacaaaataa gcagccacag agtgatcgcc
    37381 acaccagtaa agcgataacc gtcggtccac agcagcggtt tccagtattc atgtaagatt
    37441 tcgatcacag gtcagccctc ttcacaccca cggagtagcg gcgctcaagg aacagcagca
    37501 caccattgga aacggtggtg aaaaccaggt aaatcacgcc acagacgatg gcgaaataga
    37561 acggttccca ggtacttttg cctgccagct gcgtggcttt gaccacatct tccaggccga
    37621 gtaacgaaac cagtgcggta gatttgagga tcacctgcca gttgttgcca atgcctggca
    37681 gggcgtaacg catcatcgcc ggaaacatga tcctccgaaa cacttgcccg cgagtaaaac
    37741 cgaacgccgt cgccgcctct atatgccctt tcggcactgc cataaacgcg ccacggaacg
    37801 tttcggtaaa ataagcaccg tagataaaac cgagagtgat aataccagcg accatcggat
    37861 cgatatcaat ctgcccgacg cccatcgcct ccgtcaccat gtttagcgca atctgcaaac
    37921 catagaaaat cagcagcatc aacaccaagt ccggcacgcc tcgaatcagc gtggtgtacc
    37981 cttcgaaaat cagccccaaa agccgatttt gcgagagctt accgccagcg ccaattaaac
    38041 cgatgattac agcgagcact acagagctga tagccagctc cagcgtgacg agcgcaccct
    38101 gtaaaataac acctgaaaac ccatacaaca tgctgcctgt cctgtcgtgt gtggtgaatt
    38161 accgcctctt gtctccctcc ggttacccga agggagcggg ctttctttca ccgatggcga
    38221 ttaaccacca taaacatcaa aatcgaagta ctttttcgct aatttctcgt aagtaccgtc
    38281 agcgcgcatt tcggcaaagg ctttgttcag tgcctcgcgc agttcgttat cttctttacg
    38341 caggcccatg ccggtcccta cgccaaacag tttttcatct ttaacagacg gaccaccgaa
    38401 tttgtaatct ttaccgacgg gttgtttgag gaaaccttcg ctggcagcga cctcatcctg
    38461 gaacgcggca tcaatacgtc cggcagtcag gtcagaataa atgttgtcct gcccctgata
    38521 cgagacgatt tcaatgcctt ttggtgccca gtgttcatta ccgaacgtct cctgggtggt
    38581 gccctgcaat acgcctaccc gtttgccttt cagcgactcg actgtcggct gaatgtcaga
    38641 atttttcgcc accaccagac gagaatcggc agcgtacagt ttgtcggtga aggcaatttc
    38701 ttgctgacgt ttttccgtaa tggaaagcga tgacatgatg gcgtcaatct tcttcgcttt
    38761 taaggacggg attaacgcat ccagcgggtt ttcgacaaac gtacattgcg tattgatgcg
    38821 cttgcacaac tccttcgcca gctcgatatc gaaaccaacc agttcgcctt gtgaattttt
    38881 tgattcaaat ggcgcatagg tcgggtcggt accgatgcgg atgttttgcg gaattgcagc
    38941 aagtgccgca gttgcgctgg agaaggccag aaccagagag agcgatagca ccagtttttt
    39001 cataatgtcc tcaactgaca gtctttttat agggaatatt tacaggtttg ttacacttat
    39061 cgtgccataa aattgaccat caaggcaaaa tatcccgccc cgttagacgt attatttttc
    39121 attaaaaatg cttaagtatg catttcaatg caccacgata gtgcaacaaa cctggcgtgc
    39181 accatgatga tcattccatc aggtacagct tcccagcgac gtatcagtca ccgtagacat
    39241 taaagtcgaa atactttttc gccatcttgt cgtaggtgcc gtcctgacgc agctcgccaa
    39301 gcgccttatt gaaggcagcc gtcagttcag catcatcttt acgtagccct acaccggtgc
    39361 catcaccgaa gtattttttg tcttttactg atgagccagc aaaggcgaaa tctttaccag
    39421 caggttgctt gaggaatcct tcgctggcag caacttcatc ttgtaacgca gcatccagac
    39481 gtcctgcagc cagatcggaa tagaccaaat cctggttggc ataggccacc acatccacgc
    39541 ctttactacg ccaggtctcg ttagcgtaag cttcctgggt tgatccctgc agcacaccaa
    39601 catgtttacc tttcagtgaa tccagcgttg gctgaatcgg tgaacctttg gcagcaatca
    39661 aacgagaatc tgcggcgtac agcttgtcgg agaaggcaat ctcctgctga cgtttatcgg
    39721 taatggaaag cgacgaaata atagcgtcga tttttttcgc tttcagtgag gggagcagcg
    39781 cgtcaaagtc actggcaacc caggtacatt tcacctgcat ccgtttgcac atctcgttac
    39841 cgagatcgat atcaaagcca acaaaatcac ctttagcatc tttcgacgag aacggcgcat
    39901 aggtggtatc ggttccgata cgtaccgtct ccggtagcgc cgcatagctg gaagccgctg
    39961 cggagagacc gactaataaa gacagagcga gaatcgactt cttcatacat aaccctcaag
    40021 tgaaaatggc ttttttatgt tgtgtattgt gttgtgtgtt tgcaggctct ttcatgcagg
    40081 tcttatgcca tcttgacgac aacagtaaca ttcaacgtta aatatgttaa taagacgttg
    40141 cattattgtc ctgaagttga agatagcagg tatagcggtt gaatcgcagc gtttcgattg
    40201 aatggcagaa acaaaatgtc gaggatttga tcgcggttgc aaaattgccc tgaaacaggg
    40261 caacagcgga gttatgcgcc ctgccagcgg gcaaagagat cttcaggaag ggttattgca
    40321 aactggtcaa gaacacgatt aaccgtctga tttatcacat catcaaggga ttgcgggcga
    40381 tgataaaacg ccggaacggg aggcataatc accgcaccga tttctgccgc ctgagtcatt
    40441 aaacgcagat ggcctaagtg caatggtgtt tcacgcacgc agagcaccaa cgggcgacgc
    40501 tctttcagca ccacatctgc cgcacgggtc agtaagccat cagtatagct atggacaatg
    40561 ccggacaggg ttttgattga acagggtaaa atcaccatcc ccagcgtctg gaaagaaccg
    40621 gaagagatgc tggcggaaat atcgcgcgca tcgagcgtga catcagctaa tgcctgcact
    40681 tcgcgcaggg aaaaatccgt ttcgagggat aaggtctggc gcgccgcctg gctcatcacc
    40741 agatgcgttt cgatatctgt gacatcgcgc agaacctgta ataagcgcac gccataaatc
    40801 gcgccgctgg caccgctgat gcctacaatg agtcgtttca taaatagttg ccctgtcaga
    40861 cttgcaggca gactttgcag tatttcggcg ggagttgcaa gtcagggtgc cagaccggca
    40921 ccctcagcga aggcatcatc cttcgttatg catttcgaga ttttcctctt cgttctgacg
    40981 ttgcactgct ttggcgtcat cattacgtaa cgtatcgagg aaatcgaggt agccctgatc
    41041 aacatctttg gtgacgtaga cgccgttgaa caccgagcat tcaaactgct ggatatccgg
    41101 attttcagcg cgaacggcgt cgatcagatc gttcagatcc tggaaaatca acccgtcagc
    41161 accgatgatc tggcgaattt catcaacttc gcgaccgtga gcgatcagtt ccgtggcgct
    41221 cggcatatca ataccataaa cgttcgggaa gcgaatttcc ggtgccgcag aagcgaggta
    41281 cactttcttc gctccggctt cgcgtgccat ctcgataatc tgctcagaag tggtgccacg
    41341 gacgatggag tcgtcgacca gcaggacgtt tttatcgcgg aactcggcgc ggttggcatt
    41401 cagtttacgg cgcacggact tacgacgcag ctgctggccc ggcatgataa aggtgcggcc
    41461 aacatagcgg tttttaacga agccctggcg gtacggtttg cccagaatac gagcaatttc
    41521 cagcgcgata tcacacgagg tttctgggat cgggatcacc acgtcgatat ccagatcttc
    41581 ccattcgcgg gcaattttct cgcccagttt cgtgcccata ttcacacgcg cgctgtaaac
    41641 ggaaattttg tcgataaacg agtccgggcg ggcaaagtat acatactcaa acaggcacgg
    41701 attgctgacc ggattgtcag cacattgacg ggtaaacaac tgcccttctt cagtgatgta
    41761 aatcgcttcg cccggcgcga cgtcacgcag gaaatcaaag cccagcgtat cgagcgctac
    41821 gctttcggaa gcgaccatat attctgtacg gttctcgtca atatcacgtt ttcccagtac
    41881 cagcggacga atcccgtttg gatcgcggaa agcaaccata ccgtggccga taatcatcgc
    41941 cacacaggca tacgcgccgc ggattaagcg gtttgtggca gcaatggcag cgaaaatatt
    42001 gtcggcttcc agcgggtagt ggcggaagtt gtccagctcg ctggcgaaga tattaagcag
    42061 aatttccgag tcggaagtgg tgttgatgtg gcggcgtttt tcttcaaaca gttttttacg
    42121 caactcgtga gcgttggtca gattgccgtt gtgggcaagc gtaatgccat acggggagtt
    42181 aacgtaaaac ggctgcgctt cagaggcgct ggagctgcca gccgtagggt aacgcacatg
    42241 accaatgccc atattgccct gcaaacgctg catatggcga gcttcaaata catcgctcac
    42301 cagcccgttc gctttacgca aacggaagca gttattggca tctatggtga tgatgccggc
    42361 ggcatcctga ccgcgatgct gaagcaccgt taaggcatca taaatcgact ggttaaccgg
    42421 cataacaccg gcgataccga caataccgca catacgtctt tttcctcgtt aagccacatc
    42481 tcagagcact tacgctctgg gcaagaaact tgacgagctt tgcagataat caaaaaacca
    42541 tctgatgata aaactgaatt gcgggatcag ctgtgatttg ctccagtctt cacttttcga
    42601 caccccggta aaggagtcga gaaagaagag aatggcggca acaatcaaca caccgcgcaa
    42661 cgcaccgaaa cagacgccca gcacccgatc ggtgcctgac aaccccgttt tctccaccaa
    42721 ctggcctatc acgaagttca cgatagcacc aacgatcagg gtagcgataa acagtaccgc
    42781 gatggcaatc ccatttcgaa ccagttcgtc ttcaaagccc gtaaaccaga ctgacaggta
    42841 agtgtagtaa tgactggcaa caaagaaagc acaaccccat gtcaccagcg ataacgcttc
    42901 acgaacaaag ccgcggatca ggctaaccag agaggaaaaa gcaatcaccg cgattatggc
    42961 gtaatcaatc cagaccatat gtgtcccacg attttacgcc ctgtcatcct gttcggggcg
    43021 cattctaaca gaaaaagaaa acgtttgcgt agggatttcc ttcccgcgca tcaataaaaa
    43081 tggcgctgaa aaaatattca acgccatcga ctttttatgc ctttgcggca tcgtgcaatg
    43141 cgtgtcggat gcggcgtaaa cgccttatcc gacctacggt tctaacactg cccaagcctg
    43201 ataagacgcg ccagcgtcgc atcaggcaag actgtgttaa ttcggcgtat agcccattac
    43261 cacgccactt aagccagaaa gttgcttcaa ctcacccagc gaacctttca gcttatcttt
    43321 cgaggcatcc ggcccaacca gaatacgggt aattttaccc tgcactggcg tggatggcga
    43381 cgtataaacc cgataaccgg caccgcgcag cttaccgaca atctcattca ctttatcggc
    43441 atttttcagc gcacccagtt gcacaacata ggctttaccc gttggcgctg ctttttcttc
    43501 cacgaccggc ttcggttctg gcttcggcgc aggtggtgct tcaacctttg gtttaggcgg
    43561 ctccaccggt ttcggcttcg gtggggcgac cggtgcaggt tccggttcaa actcggtgtt
    43621 attggctgca atagtggccg gatcgagcga cggtgctgcg gcatcacctg cccgcacctc
    43681 ttccgctgcg ccttccggcg gctgcgtcgg caacgcctgg gtggcggctg gcatcatatc
    43741 aggctcatca cgatcgcccg ctttcggcac cagcgggata gccgcgaact catcctgata
    43801 atgttttttc tgcccgtcca gcagccctgg aagtacaatc acccccagcg ccaccagcac
    43861 gatcgtgccc actaaccgat tctgaaactt acttgccacc gcttctcctc gcgtcaatca
    43921 cttccatgac atgtgcgacc gtgtggaaag aaccacacac cagcacggtg tcttccgctt
    43981 tagcgtccgc cattgcggca tcccatgcct gcgcaacgct atcaaatgat ttgccgttac
    44041 ccaaatgctc aagcagttgt tctgccgtgg caccgcgcgg cccttccagt ggcgcacaat
    44101 accagtcatc aaccacgctt ttcaaccagg ccagagttcc ggcaatatct ttatcatgta
    44161 gcataccgat aaccgccagc acgcgcccgt ttttcggtag cgctttcata cgcccggtga
    44221 gatattccgc cgcatgtgga ttatgcgcga catcaaaaat aacgcgtggc gactcgctca
    44281 caatctggaa acgtcccggc aaaattgcgc tggcaatccc gtcgcgaatg gcattttcac
    44341 tgacttccag cccgctggca cgcagtgccg ccagcgccgt tgcggcattc ggttgcggga
    44401 caagcggcaa cggcagattt tccagcgtgc cgtgagcatc gctaaacgcc cagtcatgat
    44461 cggtgacgga atagttccac tcaacgcccc gacgttgtaa cagtgcacct ttttcctgcg
    44521 ccacatcagc aatggtagaa ggcatttccg gctcaccgac aattgccggt ttttcgctgc
    44581 ggaagatgcc tgctttctcg cgaccaatac tttcgcgatc tggacccagc cagtcggtat
    44641 gatccagcgc aatactggtt actaccgcgg catcggcatc gacaatattg gttgcgtcca
    44701 gacgaccgcc cagccctact tccagaatca ccacgtcaag ttgtgcctgc ttgaacagcc
    44761 acaacgccga cagcgtaccg tactcgaaat aggtcaggga aatatcaccg cgtgccgatt
    44821 caatctccgc aaaagaggcg gtgtgggccg attccggcaa ttcccggccc tgcacacgta
    44881 cgcgctcggt ataacgcacc agatgaggcg aactgtagac gcccactttg taccctgccg
    44941 ccatcagaat cgactccagc gtacggcagg tggtgccttt gccattcgta cccgcaacgg
    45001 taaacacaaa tggcgctggt ttcaggacgc caagacgcgc cgcgacctgg ctcacgcgct
    45061 caaggccgag ctcgatagtt ttactgtgca ggttttccag ataagaaagc cacgaagcca
    45121 gaggcgacgc ggcttgagga gtgcgtttga taatcatggt atccgctgat tcgttacggt
    45181 gagaatagca aaagggcaga gccagtggcc ctgcccttat cagttatcag gcctcaggtt
    45241 cctgatccgg taccgggggt accactacgc cttcacgcgg cgcttcagga ttcggcgctg
    45301 gcagattcat caacttcgcc agaatgctcg ccagtttcag gcgcatttcc ggacgacgga
    45361 cgatcatgtc gatcgcgcct ttctcgatca ggaattcact gcgctggaat ctaggcggca
    45421 gtttttcgcg aacggtctgt tcgataacac gcggaccggc aaagccgatt aacgctttag
    45481 gttcagcgat gttgagatcg cccagcatgg cgaaacttgc agaaacaccg cccatcgtcg
    45541 ggtcggtcag cacggagatg tacggcaagc cgcgctcctg catttttgcc agtgccgcag
    45601 aggttttcgc catctgcatc agcgacatca gtgcttcctg catacgtgcg ccaccagagg
    45661 cggagaagca gatcagcggg cagttatctt cctgcgcctg ctcaacggca cgcacgaaac
    45721 gtgcacccac aacagacccc attgaaccgc ccataaaggc gaactcgaat gccgcagcga
    45781 caaccggcat tccatacaga gtgcctttca tcaccaccag cgcatctttt tcgccggttt
    45841 ctttctgcgc agatgccaga cggtctttat acttcttgga gtcacgaaac ttcagctcat
    45901 ctttcggctc aagctcgcta cccagctcca caaggcttcc ttcatctaac aggctatgca
    45961 ggcgattacg cgctgtcata cgcatgtgat ggtcacactt cggacagacc tcaagattac
    46021 gttccagctc agcgcggtat aaaacctgac cgcagctatc acacttagtc cacacccctt
    46081 caggaatgct cgccttgcgg gtgggagtaa tgttgctttt aattcgttca atccagctca
    46141 ttagggacct ttctgtctga acctggttcg atgccagttt tatctttggg gacgcataat
    46201 gccatttttg cccccaacag accatgaatg ttgcacatta aaacataaca gcccgaaact
    46261 ttggataaaa aagtggtcga accgcggagt tactttttat tttgcggcgc gtgccgcagc
    46321 gcgtttgtga cggattattt cgatgacgcc cggcaaaata gaaaccacaa taatcccgac
    46381 gatcagcagc ttaaggttat cctgaaccat cgggattgta ccgaagaaat agcctgcgta
    46441 ggtaaaaagc agtacccaca acagtgcgcc gatcacgtta taagcagcga aatgacggta
    46501 cgacatgtgg cccattcccg caacaaacgg ggcgaacgtt ctgacgatcg gcacaaaacg
    46561 ggcgagaata atcgttttgc cgccatgttt ctcataaaac tgatgggttt tgtcgagata
    46621 actgcgacgg aaaattttcg aattgggatt actgaataac ttttcaccga acaaccgccc
    46681 gatagtgtaa ttgaccgcgt cacccacaat cgcggcgatc agcatcagca ctaccatcat
    46741 atggacgttg agatcgttgg tttccagtga tgccaacgct ccggcaacaa acagcaatga
    46801 atcacccggt aaaaacggca ttaccaccag accggtttca cagaacaaaa ttaagaacaa
    46861 aatggcataa acccagacgc cgtactccgc gactagctct gccagatgca cgtcaatgtg
    46921 caggataaaa tcgatgagga aataaatcag gtccatattt gcctatgcct tgtactcgtc
    46981 atctttcagg ctgtaactac gttggctaca gcctgaaatg ttccgagcat tattctcgtt
    47041 agtccgccag aaatagcggg cccattggcg gttttggaag atcataccgg tcagggtaat
    47101 ccaccgcgac cagatacagc ccttccgctt ttgccgttgc tgccgccagc gttctgtcct
    47161 ttgccgccag caactctgct atccagctct ccggctggtt gtgggcaccg acttccatca
    47221 ggctgccgac aatattcctg accatatgat gtacaaaggc attcgctttg atatcaacca
    47281 ccacataagg accgtgacgc gtgacattaa tatgcataac gttgcgccac ggggttcggg
    47341 actggcactg caccgcacgg aacgaggtga aatcattctc gcccagcaag cattgcgcag
    47401 cccgatgcat ccgttcagcg tccagcggtt cgtaaaaatg ggttacccct ttactcagta
    47461 ccgccgggcg cagccgatga ttgtagatga tgtagcgata acggcgagcc gtggcgctaa
    47521 atcgggcatg aaaatcatca ggtacagctt taacccaacg cacggcgatg tcaccaggta
    47581 aattcgcatt tacgcccaag gtccacgccg cgtctttgcg ctgcgcggtt gtttcgaaat
    47641 gcaccacttg cccggtaccg tgtacccctg cgtcagtacg cccggcgcag aagacggtga
    47701 tgggttcgtt cgccacctgg gagagcgcct tttccagctt ctcctgcaca ctgcggactt
    47761 cattctgccg ttgccagccg taatacttac tgccgtcgta ctcaatgccc agcgcaattt
    47821 tataaactgg cagttgttgc tggtcggaca ttagtacaga tactcctgca ccagtttctc
    47881 ggcgattttt actgccatca gcgcgccgcc aaagcgaacg ttatcggcca ccgaccagag
    47941 ctggacttgc tccggcatac cgtagtcatt acgcacgcag ccaacagaaa gatgcggcgt
    48001 acccgaagca tcccctacct gagtcgggaa ttcgttctct tcagagagca caatatcttc
    48061 gccctgagca aacgcatcac gcgcttcttc tgccgccagc ggacgcagtg cttcaaagtt
    48121 gaccatctgg gcatgaccgt agaataccgg tgcctggacg acgcttgccg aaatcatcag
    48181 cccttcgtcc tgcaggattt tgcgtacttc gtcaacgata cgacgttctt cacgcacgct
    48241 accttcgcta tccggcaaca acggcagcat attgaacgcc agttgacgac cgaagaaatc
    48301 ttcttcgtca atcggaatgc cgttgagcaa tttcgcactc tgccccgcta acgcatcgac
    48361 cgcttttttg ccctgggcgg aggctgaaat caggctggta acactgatac gcgacaaacc
    48421 gccctgatcg attaacggtt tcagtgccgc cagcagctgg ctggtcagac tgtctggtac
    48481 ggcgatgaca ttccggttgc ggtaatcggt cagtacaaac gggtttactt ccggcaccac
    48541 cagcggtacg tcgggttcga gagcaaacag tccgctgctg tcgatcacca ggcaacctga
    48601 gttggtcgct tcttcaaccc aggcagcggt agcttctttg cctgcgacaa aaaatgccag
    48661 ctgcgcctgc gtccagtcga attcagcggc atcctgcacg gtgattgttt taccaccaaa
    48721 gcgcagttgt tcgcctgcgc tttcgttacg tgccagtgca taaatttccc caaccgggaa
    48781 ctgacgttca gccagcgttt caagcagggc ttcgcccata gcgccagttg cgcccaggac
    48841 ggcaatgttc cagccttcag acatggtggt ttactccaga aatagcaaag ctccctgcca
    48901 atgttacagc agagagcatg aagaagagat taacgtgccg gatgatgaac ggcgttaaaa
    48961 cccagtttac acagcaatga tgccgcactg gcgtcatcac aaattacata cagagatgac
    49021 cattcacggc gctcaagata gtttttgcgc agtttatcga actcacccgg tatcccggcg
    49081 actttacgca gcggtgcgtc atcgcggcgc acatcataca ccaaatgcac cagccttttc
    49141 agcgtcggtt gatcgagcgg accatgcagc gtaatgcggc caaactcagg cgcgggcagc
    49201 aacgtatcca gcgcaacgtg ttgttcatgc ccaataaact tgctataggc ttcaaacact
    49261 tgcgtcgtgc cacgcgcttt accctccagg gtatagcctg cgatatgcgg cgtgccgata
    49321 tccacttttg tcagcaattc cacattaagt tccggctcgc cttcccagac atccagcacc
    49381 acgcttaact tctggccttc attcaggcag gtcagcagcg cggtattatc gacgactgcg
    49441 ccacggcagg cgttaatcag aatcgctccg ggcttcaggc tacggatcag tttttcatcc
    49501 gccagatgta gcgttttgta cggaccatct ttaaagagtg gcgtatggaa agtcagaata
    49561 tccgcgcgct ggactaactc atccagcgag cggaaatcac cctcatcccc acggtcggcg
    49621 cgaggcggat cgcaaagtaa ggttttaatc cctaacgctt ccagtcgcgc ctgcaatcga
    49681 cgtccaacgt taccaacgcc cacgatcccc accgtacggt cgtgcagtga aaatccatcg
    49741 cgttcggcaa gcatcagcag ggaggagaaa acatattcca ccaccgcaat cgcattacag
    49801 ccaggtgcag cggaaaaacc aattcccgcc tgcttcaacc atgcttcatc gacatggtcg
    49861 gtccccgcag tggcagttcc aacaaattta atgggttttc ctgccagcaa agattcattc
    49921 actttcgtga ccgaacgcac catcagcgca tccgcgtctg ccagttgagc gacggggatt
    49981 ggacgcccgg gaaccgcgat cacctcaccc aaacggctaa ataagtcgcg ggcataaggc
    50041 atattttcat caacaaggat tttcacgttt gtgttacctg tatgagacga gagttaaccg
    50101 gacaagtgtg ccataatctc gcggccaggc atacttgcga agatttcagg tataaggata
    50161 cgtaatgata caacctattt ccggccctcc tcctgggcag ccaccaggtc agggagataa
    50221 cctgccgtct ggcgcgggca atcagccttt atccagtcag caacgtactt cgctggaaag
    50281 cttaatgacg aaagtgacct cactgacgca acaacaaaga gcagaactgt gggcgggtat
    50341 caggcacgat attggtctgt cgggagattc accgctgctt tcgcgtcact tccctgccgc
    50401 tgagcataat ctggcgcaac gtctgctggc cgcgcaaaaa agccattctg cccgccagct
    50461 tttagcgcaa ttaggggagt atttacgtct ggggaataat cgtcaggcgg tcacggatta
    50521 tatccgtcat aactttggtc agacgccgct gaatcagctc tcaccggagc aattaaaaac
    50581 cattctcacc ctgttgcagg aagggaagat ggttattccg caaccacagc agcgcgaggc
    50641 gaccgaccgt cctttattac cggcggagca caatgcgcta aaacagctgg tgaccaaact
    50701 tgcggcggca acgggggaac ccagcaaaca gatctggcaa tcgatgctgg aactttccgg
    50761 ggtgaaagat ggcgagttaa ttccagcgaa actgtttaac catctggtga cctggctaca
    50821 ggcgcgtcag acgctaagcc agcaaaatac gccgacgctg gaatcactac agatggcgct
    50881 aaaacaacct ttagatgcca gtgaactggc ggcgttatcg gcatatatcc agcaaaaata
    50941 tggcctttct gcgcaatcat cgctttcttc tgcccaggcc gaggatattc ttaatcagct
    51001 ttatcaacgg cgggttaaag ggattgatcc gcgtgatatg caaccgctgc ttaatccttt
    51061 tccaccgatg atggacacgt tgcaaaatat gccaacgcgt cccgcgctgt ggatactgtt
    51121 agtcgcgatt atcctgatgc tggtctggct ggttcgttaa ccccgacgaa atgacagtat
    51181 cgtgacaata atacccagca ccgcagagat cgccccggca agaaataccg aagagtaacc
    51241 aaacgtggtc gccagcattc ccgccagcgg cccggagacg ccgagggcga tatcctgaaa
    51301 cgcggcgtaa ccgcccagtg cggtgccgcg aacttgtgag gggacgcgtt taaccacctc
    51361 cacgcccagc gcaggaaaga taagcgaaca tccggctccg gttaacgccg cgcccgctaa
    51421 tgcgacccat gcacctgggg cttgccagag cagcaacaag cccaccgttt ctacaagcag
    51481 agagacaatc gccactttca cgccgccaaa acggtccggc atccagccaa acatgacgcg
    51541 catcacgaca aatgcgccgc caaacgcggt aagagtaaag cccgccatcg cccatccttt
    51601 gctggcaaag tagagcgaaa cgaaagtccc gataaccgca aaaccaacgc cttgtagtgc
    51661 cagccctaac cctggtttcc agataagccc gacaacgctc cacagcgatg gacgttctcc
    51721 cgccagggcc ggtactttgc gcactgtgcc gttacaggcc cacgccagta agggtaatac
    51781 cattgtggtg atcgccagtg cggcaaaacc gtaatggcta tgaatcaaca ggccaagcgg
    51841 cgcaccaaca gcgagggcac cgtaaatcgc cattccgttc catgacatca ctttgccaga
    51901 gtgttttggc cctacgatgc ctaaccccca ggtcagagcg cctgtcagta actggctttc
    51961 accaaaccca agaatcaaac gcccgatgac caacagggca aatttgaacg gtgcggagac
    52021 aggcaaaatc gccgccagca gcaacgcgcc gccagccaga ccacaagcta acatcccctg
    52081 aagcgccgaa cgttttgcgc catattgatc ggccagtcgc ccggcgtaac cacgcgtcag
    52141 caccgtagcc agaaactgaa tcccgacggc aatgccgacc atggtattgc catagcccag
    52201 atcatgatga acaaacagcg ggataaccgg caacggcaac cctacggtca tgtaggtgag
    52261 aaaaaccgca aaagcgatgc ggaagagcga aaaactggca gaagatcgtg tttcggtttg
    52321 gcttacagca gtcatgcatt actccagaat gcagcgcaag gcgaggagta tccccgtctc
    52381 atctctctgg tttcagggtt acggtgcgtt ggcaggattt aacgcgtacg tcttttcaga
    52441 aggaaatcga caaagcggga agtttgccta gaactggtgg cgattgtcaa tgatgtgaaa
    52501 aagggaacca tcaggttccc ttttgcgtta gtgccggagg ccgcattggc gcgtaacgtc
    52561 ggatgcgacg ctggcgcgtc ttatccgacc tactccggcc tactgcgaat taatctttca
    52621 gcttgcgcat taccagcgtg gcgttggtgc cgccgaagcc gaagctgtta gacataacag
    52681 tggtcagttc gcgatcggtc gtttcggtca cgatgttcag acccgcagcc tgctcgtcca
    52741 gctcttcaat gttgatgctc ggggcgataa agccgtgttc cagcatcagc agagagtaga
    52801 tagcttcctg cacacccgca gcgcccagtg agtgaccggt catggctttg gttgcagaaa
    52861 tcgccgggct cttatcgccg aacacttcac ggatagctgc cagctctttc acgtcgccaa
    52921 ccggagtcga agtaccgtga gagttcaggt aatcgattgg ggtatcaacg ccatgcatcg
    52981 ccatctgcat acaacgtact gcgccttcgc cagacggagc aaccatgtct gcaccatcag
    53041 aggttgcgcc gtagccaacg atttcagcat agatgtaagc accacgcgcc agcgcgtgtt
    53101 ccagctcttc aaccactacc ataccgccgc cgccagcgat aacgaaaccg tcacggtgag
    53161 cgtcgtaagt acgggaggct ttttccgggg tgtcgttgta tttagtagac agcgcaccca
    53221 tcgcgtcgaa ttcgcaggcc atttcccagc acagctcttc gccgccgcca gcaaacacga
    53281 tgtcctgttt gcccagttgg atctgctcta ctgcgttacc gatacagtgt gcggaagtcg
    53341 cacacgcgga gctgatggag tagttaacgc catgaatttt aaacggcgtg gcgagacagg
    53401 cagaaacgcc ggatgccatc gctttagtga ccacatacgg gccaaccgct ttcaggccgc
    53461 gcgggccgcg cattgcgtca gcgccgaaca cctggaaacg cggggaaccg ccgccggaac
    53521 ctgcaatcag gccaacgcgc gggttattct ggtaagcttc cggagagagg cccgcatcag
    53581 cgattgcctg ctccatagaa aggaatgcat aaatggatgc gtcgctcata aagcgcacaa
    53641 ctttgcggtc aatgaggcca gtgatatcca gttttatgtt gccccagacg tggctacgca
    53701 tgccggaatc cttcagctcc tgagagaaag tgatccctga acgtccttca cgcagagatg
    53761 ccaggacttc ctgctggtta ttaccgatgc tggaaacaat gcccaggcca gtaatcactg
    53821 cacgtttcat tcaatacctc tgtaagtcgc acatagagta agtttcgaat gcacaatagc
    53881 gtacacttgt acgccgaaca agtccgatca gccatttaat agagaaattt gcgcagcctt
    53941 acacacatcg ctaagatcga gccaccgcct gtaagacgag taacttacgt gaaacactac
    54001 tccatacaac ctgccaacct cgaatttaat gctgagggta cacctgtttc ccgagatttt
    54061 gacgatgtct atttttccaa cgataacggg ctggaagaga cgcgttatgt ttttctggga
    54121 ggtaaccaat tagaggtacg ctttcctgag catccacatc ctctgtttgt ggtagcagag
    54181 agcggcttcg gcaccggatt aaacttcctg acgctatggc aggcatttga tcagtttcgc
    54241 gaagcgcatc cgcaagcgca attacaacgc ttacatttca ttagttttga gaaatttccc
    54301 ctcacccgtg cggatttagc cttagcgcat caacactggc cggaactggc tccgtgggca
    54361 gaacaacttc aggcgcagtg gccaatgccc ttgcccggtt gccatcgttt attgctcgat
    54421 gaaggccgcg tgacgctgga tttatggttt ggcgatatta acgaactgac cagccaactg
    54481 gacgattcgc taaatcaaaa agtagatgcc tggtttctgg acggctttgc gccagctaaa
    54541 aacccggata tgtggacgca aaatctgttt aacgccatgg caaggttggc gcgtccaggc
    54601 ggcacgctgg cgacatttac gtctgccggt tttgtccgcc gcggtttgca ggaagccgga
    54661 ttcacgatgc aaaaacgtaa gggctttggg cgcaaacggg aaatgctttg tggggtgatg
    54721 gaacagacat taccactccc ctgctccacg ccgtggttta atcgtacggg cagcagcaaa
    54781 cgggaagtag cgattatcgg cggtggtatt gccagcgcgt tgttgtcgct ggcgttatta
    54841 cggcgcggct ggcaggtagc gctttattgc gcggatgaag cccccgcgct gggtgcttcc
    54901 ggcaatcgcc agggggcgct gtatccgtta ttaagcaaac acgatgaggc gctaaaccgc
    54961 tttttctcta atgcgtttac ttttgctcgt cggttttacg accaattacc cgttaaattt
    55021 gatcatgact ggtgcggcgt cacgcagtta ggctgggatg agaaaagcca gcataaaatc
    55081 gcacagatgt tgtcaatgga tttacccgca gaactggctg tagccgttga ggcaaatgcg
    55141 gttgaacaaa ttacgggcgt tgcgacaaat tgcagcggca ttacttatcc gcaaggtggt
    55201 tggctgtgcc cagcagaact gacccgtaat gtgctggaac tggcgcaaca gcagggtttg
    55261 cagatttatt atcaatatca gttacagaat ttatcccgta aggatgactg ttggttgttg
    55321 aattttgcag gagatcagca agcaacacac agcgtagtgg tactggcgaa cgggcatcaa
    55381 atcagccgtt tcagccaaac gtcgactctc ccggtgtatt cggttgccgg gcaggtcagc
    55441 catattccga caacgccgga actggcagag ctgaagcagg tgctgtgcta tgacggttat
    55501 ctcacgccac aaaatccggc gaatcaacat cattgtattg gtgccagtta tcatcgcggc
    55561 agcgaagata cggcgtacag tgaggacgat cagcagcaga atcgccagcg gttgattgat
    55621 tgtttcccgc aggcacagtg ggcaaaagag gttgatgtca gtgataaaga ggcgcgctgc
    55681 ggtgtgcgtt gtgccacccg cgatcatctg ccaatggtag gcaatgttcc cgattatgag
    55741 gcaacactcg tggaatatgc gtcgttggcg gagcaaaaag atgaggcggt aagcgcgccg
    55801 gtttttgacg atctctttat gtttgcggct ttaggttctc gcggtttgtg ttctgccccg
    55861 ctgtgtgccg agattctggc ggcacagatg agcgacgaac cgattccgat ggatgccagt
    55921 acgctggcgg cgttaaaccc gaatcggtta tgggtgcgga aattgttgaa gggtaaagcg
    55981 gttaaggcgc ggtaatctgc tctcgcagtg tttgccggat gcggcgtgaa cgccttatcc
    56041 aacctacgtg tgacgtgtag gcctgataag acgcgataag cgtcgcatca ggcatggtgc
    56101 tccagacgcc gcaacttaca gctgtgacgc ctgctgaaac aggttttccc acatatcggt
    56161 caccagcgcc tggtcacgcg gcgacaattc accggcacca atggcttttt ccagactctg
    56221 gctaacggtc gtatgcaccg cctgagcgga gtggtcatca ccactttcca gttctgcgat
    56281 ggctaatgtc aggtggccac gcaaataccc actggcaaac aactcatcat cactggcatg
    56341 gtcaaccatg ccgtcgatta atgccagaat gcgtgattca aactccgcga tcatcttctt
    56401 tcctcattgt aaaacgtggc gctggcttca gttgagcgct tccggccacg ggaactgttc
    56461 cgccgtaagt tccggcgtgt gataataatt ttgtagtgct ttaatgaagc gtgccgggcg
    56521 ttccggaatg ccatttgtca gataatccat cacctgcgca tgtacccgcc gttgaaacac
    56581 cacacgatcc ggttcgaaat ccccttccag attgtcgcaa ctgacgttaa acggatatcc
    56641 agccgccacg cagaataacc agtcgaacgc ctgcggcttc acttcaacat cttcaaactg
    56701 gctttgcgtc tgggcatcgc gtccgtccgg gcaataccag tagccgaaat caaccagctc
    56761 acggcgcgct tttccggcaa tacaccagtg cgaaatctca tgaattgcgc tggcataaaa
    56821 gccatgagca aagacgattc ggttatacgg tacttccgca tcagcaggaa gatagatcgg
    56881 ttcgtcgtcg cctttaatca gacgggtatt aaattcatcg gcaaagcagc tattaaaaat
    56941 ttcaatcaac tgctcgtagt ggtgtgtact gttcattagt tcatccccaa ccagtggagg
    57001 atctcctgtc cgtggctatc gtaaagtaat ttggcactca tcaccgccga gacgataaca
    57061 atcatcgggc ggatcagctt ttgtcctttg ctcaacacca accgtgaacc catgcgcgcg
    57121 ccgaggaact gccccaccag catgacaaag cccgtcgccc aaatcacttt gccgccgaga
    57181 ataaacagca gcagaccgcc aatgtttgac gttgcgttga gtaatttggc gtgagccgtg
    57241 gctttggcga ggttgaatcc gcacagcata acgaaggcca gcgcgtaaaa cgatccggca
    57301 gccgggccaa agaatccgtc ataaaaaccg acgcagccac cggcaattaa cgcgaagggt
    57361 aaaccgtaca tccggcgctg gcgatcttct tcacccagct ttggcatcag caagaaatag
    57421 aggccgatac aaatcaccag aatgggcaaa atctgccgta agacatcagc ctgaacgtac
    57481 tgtaccaaca gtgcgccgct cattgagccg acaaaggtca tggcaatatt gagtttctga
    57541 tcgcttaaac taaccacttt gcggcgaata aagtagatag tggcggaaat agagccgccg
    57601 caggcttgca gtttattggt tgccagcgcg ttagcgggag acattcccgc agccatcaat
    57661 gccggaatgg tgagtaaccc accgccaccg gcaatcgagt cgataaatcc cgccagcatg
    57721 gcgacaaaaa agagaactcc cagcaacagc ggagaaacca taaacaggct attaaacgtt
    57781 tccattagat cacgtgctca tccagtagcg cctggcagga aggcggcaac ggaggcggtg
    57841 tcttcttctc aggctttgtt gttcccggtt ttggaggttc aaaccagctt tgcagttctg
    57901 ccccgcaacc atcgcctgat ggcggtaaag gttgatcttc acactccaga ctatcggcag
    57961 ggcaacgtaa tcgtacatgc atatgcgcgc gatgctggaa ccagggtcgc actttgcgca
    58021 accagtcgcg atcggtgccc gcatcaaggc aaagttgttg tttaatcgcc ggattaacaa
    58081 aaatgcgcgt gacgtctttg tcctgggcgg cgagtttgat caagctgaaa atttctggct
    58141 tccacagcgt ggagacaacg tgtttaccgt cgggggaaac taagtccagt gcttgcgggc
    58201 gcaagagctg cgcggaggtc cagcgagttt tcggcagttg cagaaagata tcgacatcca
    58261 gtccggtctg gtggctggca tgaccgccgt tgaaacgccc accagcgggc atccccatat
    58321 cgccaatcag caccgtaccc atgcccagat tgctcacctg gctactcaga cgctggataa
    58381 acatcaccag atccgggtga ccgaaatagc gacgctgatc ggtacgcatg acctgataat
    58441 gttcggactg tatcggcaac gtgtcagcgc cgacaataca gccattagaa aaactgccta
    58501 tcgattgtgc gctacccggc acaggttggg ttattttttg ccacggcgtc gctgccaggc
    58561 tagcgctact ggcaagcaga gccagcagcg caatcgcggt tttgttcatt ttttaccagc
    58621 gtggaatatc agtcttcaca tcggcatttt gcgcccgctg ccgtaacagg tgatccatta
    58681 aaacgatcgc cagcatcgct tcagcgatcg gcactgcacg gatcccgaca cagggatcgt
    58741 gacggccttt ggtgatcatc tcaacttctt cgccaaagcg gttaatagtg cgccccggca
    58801 cggtaatgct ggaggtcggt ttcagcgcca tatgagcaat gatttgctgc ccgctgctga
    58861 taccgccgaa aatgccgccc gcatggttgc tctggaaacc ctctttggtg atttcgtcgc
    58921 ggttctggct gccgcgcagc gccactacgt caaaaccgtc gccaatttcc acgcctttca
    58981 ccgcgttgat gctcatcagc gcatgggcga tgtcggcatc caggcggtca aagaccggct
    59041 cgccaagtcc ggcggggaca ccactggcaa caacggtgac tttcgcgccg atggagtcgc
    59101 cctctttttt cagcgcgcgc atcagttcat ctaacgcgtc gattttgtcg gggtccgggc
    59161 agaagaacgg attttgttca accagcgacc agtctttgat ttccagcgga atgtcgccca
    59221 tctgggtcag gcagccgcgg atttcaatac caaatttctc ggcgagatat tttttggcaa
    59281 tcgcccctgc cgccacgcgc atggcggttt cgcgggcgga agaacgtcca ccgccgcgat
    59341 aatcgcgcag accgtatttt tgttcgtagg tgtaatcagc atggcctgga cggaagacgt
    59401 ctttaattgc gctgtaatcc tgagaacgct ggtcggtatt ttcgatcaac aagccaatgc
    59461 tggtgccagt agtaacgcct tcaaaaacac cggagagtat tttgacctga tccggctcgc
    59521 ggcgctgggt ggtatagcgc gatgtcccag ggcgacgacg atcaaggtca tgttgcaggt
    59581 ccgcttccgt cagcggaatg cctggcggaa caccatcgac gatgcagccg agcgccagcc
    59641 cgtgcgattc gccgaaggtg gttacgcgaa agagttgtcc aattgtgttt ccagccatca
    59701 cggctccgtt attgttgtgt ttgcgtgttt acttaatctt tataaatcgc gaaatgttct
    59761 cgtgcggcaa gaagctgctc tttagtgagc ataaacacac catcgccgcc gttatcaaac
    59821 tccagccagg tgaacggaac atcgggatat tgttccataa gatgtaccat gctgttgccc
    59881 acttcacaaa tcaacacgcc atcatcagca aggtaatctg ccgcgttacc gagaatgcga
    59941 cgcgtcagtt tcaggccgtc ggtgccagat gccaggccca gttccggctc gtgacggtat
    60001 tcgtttggca ggtcggacat atcttccgcg tcgacatacg gcgggttagt gacaatcaga
    60061 tcgtattgca ctttcggcaa atcacggaac agatcggaac gaatcggaat gacgttgtgg
    60121 atcagaccgt gttcttcgat gttctgttcg gcaaccgcca gcgcgtcggg agagatatct
    60181 accgcgtcga cttccgcttc cgggaaggca taagcacagg caatggcgat gcagccgcta
    60241 ccggtacaca tatctaaaat atgctgcggt tgcttgctga tgagtccggc aaatttattg
    60301 ttgatcagtt caccaatcgg cgagcgcggc accagcacgc gttcatcgac gtaaaattca
    60361 tgaccgcaga accaggcttt gttggtcagg taagccaccg gaatgcgctc gttgacgcgg
    60421 cggatcacgc gttcaacaat acggtgtttt tcgctggagg tcagacgcgc ggtgcgcata
    60481 tcttccggga tatccagcgg caggtagagc gatggtaaca ccagctgtac ggcttcatcc
    60541 cacgggttat cggtgccgtg accgtaccag atatttgccg cgctgaagcg gcttaccgac
    60601 cagcgcaaca tgtcctgaat ggtttgcagc tcatttactg cttcatcaac gaaaatttta
    60661 tccacgtatt cctccagggc atgatcgcaa taatttcggc ggctagtttg ccatgaagat
    60721 gacgataaat cagcattcac gcgcggtgag tgaggaaaaa tacgtttaaa acgatcgatt
    60781 gtgctacgag tcgggtaaac tgtaggaaaa ttagaaatag agacagataa atgaaaaaga
    60841 aaacaacact tagcgaggag gaccaggctc tgtttcgcca gttgatggcg gggactcgca
    60901 agattaagca ggacacgatt gtccaccgac cgcagcgtaa aaaaatcagc gaagtgccgg
    60961 tgaaacgctt gatccaggag caggctgatg ccagccatta tttctccgat gagtttcagc
    61021 cgttattaaa taccgaaggt ccggtgaaat atgttcgccc ggatgtcagc cattttgagg
    61081 cgaagaaact gcgccgcggc gattattcac cggaattgtt tttggattta cacggtctga
    61141 cgcagctgca ggccaagcag gaactgggcg cgttgattgc cgcctgtcgc cgcgaacatg
    61201 tgttttgtgc ctgcgtgatg catgggcacg ggaagcatat tttgaagcaa caaacaccgc
    61261 tgtggctggc acaacatccg catgtgatgg catttcacca ggcaccgaaa gagtacggcg
    61321 gtgatgcggc tttgttggtg ttgattgaag tggaagagtg gttgccgccg gagttgccct
    61381 gaatgctggc agcaaatgcc ggagcagcgt aaacgcctta tccggcctac aaatatgtga
    61441 taattcaata aattgcaatt cacttcgtag gcctgataag acgcatcaga gcaatactac
    61501 gaacttcagt tcattcaata catactgaaa acgacacgtc gaatcaatcg gttgcccact
    61561 actggtattc acattcacca caatatcgtg tagtacttta tccgttaccg gatgacgagt
    61621 tggcgtaacc gttaccgttg atttatcaca accgtaagag gtaaaggtta acaattctga
    61681 taccgcgcgg cagtatgctc ctctgccatc attatcagaa aaattactgc catcacagag
    61741 tctgccgcct tccatcaggt aggtcgaaag cgtggtggtc actgttgacc ccgtggactg
    61801 aattcgtaac gccttattat ggctgacata attttcactc caggagatat cgtatgaagc
    61861 gaccggtatc gttgggcata ttgtgttatg cactgtaggg gccgtatagc ttccaaatcc
    61921 gggtaaatct gtataggaat aaaaggatgc gatccaaggg caaccataat catcagatgc
    61981 aacggtaatt ttaaacgtca tatatgcaca acgaaaaatt ttcgtatcta tgccagggca
    62041 atttgagtta ttaattgtat caaagtcagt cagtaccaaa gttggagctg aacccgaccc
    62101 agaccaggaa ataacccgca acgtatcgcc tttatacgca aaagaatcat atttcccata
    62161 tgcaacctcc tttggcgtta tcaccgtaac attgaaaaca ctgctcgcta cagcaccggt
    62221 taccttgtcc atggcataat aaactctcac actgtcaaaa ctcgcactca taagtttatc
    62281 tccagcataa ctcttagatg cgccgacaac actcagaacg agcagcatta ggctcgcgaa
    62341 aattatggtc cattttttca tttcagaaat actcaaaggt tgccgtcact gtggcactaa
    62401 agtctccgga cgttacatct cgcccgctct ttgcctgtaa ccaggtatta aaatttaggc
    62461 tgttatttcc ctgattcagc gggagcgtga cgcctgttgt attgttgatt ggcaccgatg
    62521 tgcctgccgc agtttcaata ccaatcccag cgccggatgc tgatgacgag gagtcaaagg
    62581 ccagaaagcc gggtagagca ctatcttctg ttcctgtcaa cgttactttg acattaaaca
    62641 atgtagaact atgacaatct ttcaattgaa acaccaatgg cactcgctct gactgcccaa
    62701 ggttcattaa atcgctggcg gcaatggcag gaaaatgtac ttctgccaac tggctaccat
    62761 taatcactgg cgtacacgat ttactgatta aattgccggt aaagtgcaaa ttatctcctg
    62821 ctattgtggt tccactgccc agcaacatca ggctactaat aaaaaacaac gtacgctttt
    62881 tcttcattgg taatctacct gtaacgtggc ccaggcctca aagtccgcct ctggcagaat
    62941 gacaccactc ttttttactg gcacagccgt taacaccggt aaatcggttt cattcaccac
    63001 aagtggcgta ttgatcgtaa aactctgccc tgcctgtttg agctgaatgc ctaaaccagt
    63061 gatattgctg cttactgcag caatatcaaa atcgcttggt gtgccactcc agctcaaggt
    63121 catttccatt aatgggtcgc gaaccgccgt atcgcaggta atggaatacg gcacggtttg
    63181 ctcataattg ctgccattaa tgtcatcaat aagcacatcc tgaaaagtta tttcaatggt
    63241 ttgagcgttg ctaatcgtgc agttaggcgg ctgcactaaa taaccgtgaa aagagacgtt
    63301 tgagagtgcc atcgagcaaa agccccacag caaaataagc gagatccgtt tcattggtaa
    63361 tccaccacaa ttgtggcact ggcgttaaat tcagctgccg ctaacgttgt accactctgt
    63421 ttcaccggca cagcttccag aacgggaact cccgaggaaa aattaaatgg caaccatgag
    63481 ccagaggtta agtcgagtat tgtgtgatca ctacttttct gaacccgaat accaaaccca
    63541 ggaatacccg tgctgataac tgtttcaccg ttaatcacga ccgttgtcgc ctgcatctgt
    63601 agacgcaggt catccattgc cagattgtta caaacaagcg agtagtcaat gggacgttta
    63661 taatcaaccc tattgatctt attaatgaat acattaccaa actcaactgc cgcaccattt
    63721 accgtacaag gaggaggggc atcgacaagc acccgtaaat tcatacttac tgacttactc
    63781 gctgcaactg caaagccgct accaacacaa catatcagta acccagttac cgcccagaca
    63841 gaacaagatc ctttcatact ggctctcccg gcctcagcct ttgcggctct gctcttcatc
    63901 gacctagcaa ctgttaccgt tgcaagtgaa aaagagcggc atccgagcgc catagtcgtt
    63961 gacataggtc agtaccggca cgctacccat cttcacattc agcggtgcat aggttttcgg
    64021 cgacatcact attggcgaga aaccggctgc cggattgcca tttttctgtg tgctggcatt
    64081 actgaaaatg acgtaatacg acgtcgggtt attgacggta aattcctgac cgttgcgggt
    64141 taaagtaacc ttatattgct aggggttttt catgctgacg ttctccagcg ccttcggtcg
    64201 ccagaacaac ttaatacgcg tttgtagtgc tatttgcagc gtgttggctt tgtttgattt
    64261 tggcgggatc tcacgcacgt tgaagtagaa caggctttca cgatcggcag gcaatttgtt
    64321 gatatccggc attccctgca cttttacctg cccgttcatc attgaatcga tgcgttgcac
    64381 cggtggcaga acagttaacg gtgaactgat cttgttaccc ttttcatctt cgatccagct
    64441 ttgggccaga taaggcagtt tcggatcgtt attgcgcaac gtcacgctga ttgatttgtc
    64501 gctttcatta aatatcaaac gggtacgatc tggcgttaca gaagcctggg cagacaaact
    64561 ggcagcgcta agcagtaacg ctagcgtcgt agcacccaat ttggcagagc aaagtaaatc
    64621 agacatggat cattccttaa tcacgttatt ggttagctga aacagaaaca gggggattgg
    64681 tgggtgtcac ctgttgcgtc tgctcctgaa tgacaggctt tatgtcatca ggaacaaccg
    64741 gcgcgacgtt gcctttatgc tggcatggca gtaacaagcc gttaaacaga tcggcaggca
    64801 gcgggtccgg caggttgata tcgcaatgcg caacaccact ccagaacaca ctcatgtgtt
    64861 cgccaggttt caccccagcc agataaacat tgccgtcatc atcgacaagg cccactgtct
    64921 gctcgttatc attttttact tctgcgccaa acggaggatg gctgccgtct tgtaaacgca
    64981 gcaccgccat cgctttttga ccactgatga cggcaaattt gcggtagcca atcgcccctt
    65041 cagttagcgt ggcttgtacc accgactggg ttgcttcggc gttttccggc agtttgttga
    65101 ggtcgatata cgcctgattg cggtaatagt tattgacgtc agaaacgacg gctttaccaa
    65161 acatattggt ataaacagca gcaccgttac cttctaccgg aacatcggca acgccatcgg
    65221 catcaatcaa caagcgtgtc ccgcccatat tctgggtacg gtgaagtgcg ccaccgtggg
    65281 cagtaagcgt tgcgccgccc tgtaacgaca agcccgcaga ggtgtattgc ccttcatggt
    65341 agttcgcact gaggtcaacc tgcgccagcg aaccatcatg gctgtaatag ccgtcaacgc
    65401 tggtgtgttt gtcactggtg ccgacgttca actgatagtg agtcgcgtca tcgacacggc
    65461 tgaaataacc gacctgactg ctgtccgtcc cactgccata gtaccgttat agctaacggt
    65521 actgttgtcg ccccacggca tactgagcga aatgtacatg cctttgtcgg cctggttgtc
    65581 atactcgtaa cggtagccag tcatcgagat gctgacatta cgaatactgc ccatattgaa
    65641 atagtgcgag agcatgatgt tgtagtttgt ctgctcctcg cgatcccagt aggtatggcg
    65701 ggtatagttg agataaaccg aaacacccgc atcgcggaag ttctggttat aagtggcggt
    65761 gtacatctct ttgtcgttgc ccgtgcgtac cattccgctg tcactggcat ccagatactc
    65821 gctcatggtc ataaagttct cttccgagaa gcgatatcca gcaaaagtaa cgcggctgtt
    65881 gagctggtca aagtctttgg aataactcac acggaaggag ttaccgtcca gcgaaccttt
    65941 gccataagcg gtgtctttat ccagtttggt gtgcgagtga gtaacatcaa acgcgaccgc
    66001 gccgaatgta gacaaatcgc gaccgacacc aagcgccgca gactgatagt tttcatctcc
    66061 cagtgcgccg ccatataacg accagccgtt agcgatcccc caggaagctt cagcaccaga
    66121 aaaaaattca ccctcgacat ggtgtcccca ctcttgcgga cggcccatca tgatcttata
    66181 gcgaacctga cctggacgag tgaggtatgg catcgaggcg gtgctgatgt catattcctg
    66241 cacctggccg ttctgttctt caatgcgaat atgcaacgta ccggagacag aatcaccaag
    66301 atcctgaata cgaaacggtc cagccggaac ctgcgtttcg taaatcacac gccccatctg
    66361 gctgacggtc acttttgctg tggtgtgtgc cacgccggaa atgtctggcg cgtagccgcg
    66421 cagattggga ggcaacattt gatcgtcagt actgacactg ccaccaacat agttaaaacc
    66481 atcaaaaata tcggatctga ggtaatcctc gcccagcgcc agtttggctt ttaatgatgg
    66541 taacgcccgc ctggcatagt agcgactcca ctcccatttt ttttgagttt catcgccgct
    66601 gaattcatcg tcatcattac tgcgagtatg ttgatagtta gtctgccagt cagcacgcat
    66661 acgccacggc cctaggttaa ccccaaccgt cccgttgccg ctgatctcgt tactatcatc
    66721 accgccattt tcttcgtgcc gggtttgtgc gttgatgctg tagtccgcga cgatcccgga
    66781 gatgccgtca tcccaacgtg aaggcggatc ccagtcggga taagtatatt cgaggtaagc
    66841 ctgtggcagt gaaatgacta atgcggactg gcttaaatca gccttaattt ccatgccttc
    66901 cagttgaccg gatttcaggc atttagcatc gtggctccat tgcagatttt tcgccacgtc
    66961 ttcttttaaa ccaaactgcg ctaccagttc cggtgtcaga caagcatagc ttttgctcgc
    67021 gtcatcttca ccagcatacc agtaaatatc gtactcttcc gccaatggct gtttatttag
    67081 ttgaacctgt aaattgtatt ttccgggctc aacatatccc tggctggaaa aacgcttcag
    67141 atcaattttt gtgtcgcctt ttaattccag aaaacgggaa tcaaactgaa tgtcgtcttc
    67201 agcccagaca ctgctataac tccctgacat tgccagcgca atgcaccacg gaagaatccg
    67261 taatcgaaaa agagaatggt caggcataca cgtttctatc cgtttttatt cttgttcggt
    67321 caaaatccat ttaaatcccc catagcagtg tcgctatggg ggtatgcctt acaggtaagt
    67381 aatctggaag gtggtatttg cttcaaaatt acccagatct ggcgcatcag ctgcgccgac
    67441 cagccaggct ttaaagttca gtgtctgctt cgctttgcct ttgttggtag aagtgtcttt
    67501 cactattttc tggtcaatac ccatgccgct tttgtaagag gtacccagag agtcaccgat
    67561 cgccaggcta acattgttaa atgccgcacc ggtatcggta ttgaaaatgg tgtaataatt
    67621 gccgctattt gcagaagaaa cggtaccagt aaaggtagtg gtcattgttt cctgagtatc
    67681 aaatacgcaa tcttgcagac gaatctggaa acttttcggc gtggtggtgc cgttattttt
    67741 cagtttttcg gcaccgatat cacccatatc aacttccagt ttgtgatcgt ccggaacgat
    67801 agaacacact gagtcttcaa tggtgccgta aaaacgtgca gtaccgttgt tacccgcagc
    67861 gattgttgcc gtagaggtga acacgcccat caccattgcc gcagcaatag ctgttttaac
    67921 aaacttactc attatttgtc cttaaactat aaataattaa aaatataaac attgcaatac
    67981 attgattcag tcaatagcca atgtttatac ccagccattg aatacggcat gcgacccaaa
    68041 aagaacaggt cgtagaaaat tgtattcatt ggtgtggggc cgcatagtga tgtttccaca
    68101 cccctgatcc aatttaattt tactaaagag taaatgtaga tttaattaat atattgacag
    68161 aagaaaggtt atcttaagga catccttaaa aaatacggaa taagtattta gaaaaaatag
    68221 aaagtatatc aataatgaat atcaacagct taaaaaacaa acaatgaaca tataatgcga
    68281 ttggcattta tccacttttg acaatataat atataaacac tatagccaga atttaatcaa
    68341 gtttcaatta catcctgcct gattattcaa ctctattttc tctattatct gtattaataa
    68401 atgacactga gtgtcaaaca gacattattc atttcaacat gtaactttgg gcaaaatcaa
    68461 gatacgcgca gaagaataat ttccgaatga atgtagaaag tgttttattt atagataagg
    68521 agagtcttcg atagtagtga ggttgacaga tatccgctgc ctaatgagca attatgccca
    68581 atgaatctac ctcattgggc ataatttgga acacatcaga tagcttttgc catcttcaga
    68641 ttacacgggc tcatctgcca gttgaatgta ccgtttccgc tttcatcaag cgttacgctg
    68701 gcaatggccg aggtggtaaa cataggcggc gtttcgcccg gacataactc ggcaaccaga
    68761 tacccgacta acggtaagtg agagatcacc agtaccgaag caacaccttc attggtcaac
    68821 gcctgcaaat aggcactgac cagaccaaca tcgccgcagg gcgttaactc cggcagaact
    68881 tctgcactgg aaggcaggtt cagacaatcc cctacttctt ccagtgtttg ctcggctcgc
    68941 aggaacgggc tcaccagaac gcgttcgatt tccacttttt gacctttcag ccagttcgcc
    69001 atcaggcgag attcgtcaca accattagtg gtcagaggac gaaccgaatc actggcggca
    69061 tcgagggctg cgtcgccgtg acgcatgata aaaacttgca tattgcaccg cttttgttaa
    69121 ccagattcgc ccgttttctt tcatctgaaa ccagagaaag aaaacggtgg ccggcattgt
    69181 gccttatcca ttcaccgaat gaaacgctgt tttttacctc aatggcgtaa gtatagtcaa
    69241 tatgcgttta cattttgacc aacactccgc cattcagcgc ggattcatat agctttgacc
    69301 ttcttattgc aggtcagttg cagttgtttt ccaaaaactt tccccacgcg cgcccatctc
    69361 gaccaaacgc tcgcaagggg taaaacggga accatactgc gtggccagtc gttgcattat
    69421 tgcaaccact tcgcccgcgc cgagagaatc gatatagcgg aacggtccac cgagaaatgg
    69481 cggaaaacca atgccaaata ccgcaccaat atccccgtca cgcacgctac ggataacctg
    69541 ctcatcaaca caacgtactg cttcattcag catcaacatc acacaccgtt cagcaacctg
    69601 cggtgcggag agtcgcccct gcccttgtgc gccaatgagc gggtaaatgg caggatcgac
    69661 ctgttttttg cttttacgcc ctttctgacc ataaagatag aaaccccgac cattttttct
    69721 gcctttgcga tcgtcgttca aaattgaaga aacaacattt gcaggcgcgc taaaacgttc
    69781 tccataagcg gcttccagta caggaataat tttagtcccg gtgtcgattc ctacctcatc
    69841 caaaagttgg attgggccta ccggaaaacc aaatttcact agcgcggcat caatgtgctc
    69901 tacccgttca ccttcggtca gcatgcggat agcttcatta atgtaaggcg ctaagatgcg
    69961 attgacgtaa aacccggctt tgtcacgcac gacaatgggc gttttgccct gttttttcgc
    70021 cagttttacc gtagtggcga tggtttgcgc cgatgtaccc gcatgaggaa taatctctac
    70081 cagcggcatt ttttccaccg gactgaagaa atgcaggcca ataacttgct caggtcgcgc
    70141 ggcgtgagcg gcgatatcac caatcggtaa agatgacgtg ttcgaagcaa aaatggtatg
    70201 agcggcgcaa ttttgctcca cttccgccac catctgttgt tttaattcga gattttcaaa
    70261 caccgcttca ataatcagat cgcgatgggc aaagccgcga tagtccgtcg ttccggagat
    70321 taatgccagc tgtttgtcac gttcgctggc tttgagatga cgacggcgaa ctttgccctc
    70381 cagctgatcc caactgtact tcagcgcatg atttatgccc tgcgggttga tatctttaat
    70441 tctgaccgga agccccgctt tacaagcagt gacataagca ataccgccgc ccatcaagcc
    70501 accacctaaa attcccacgc tgtttaatgg cgcaggcggt gcatcactac cgggatcttt
    70561 cttcacgtcc gtactggcaa aaaagatact acgcaaagct tgcgattgcg gcgtcatcgc
    70621 cagttcgcca aaggcgcgag cttcagcgtc atagccgctg ctggtgccct gcgctaatcc
    70681 cgtttcaaca acctccagga tgcgttctgt cgccggataa ttgccttgag ttttgtgttc
    70741 tgttttcttg ccgaccattt tgaacagcag cgcacgacct aacggccccg ccagaatacg
    70801 ctcgcgtaca ggtagagggc gggaagatgg gcgatccttc ttcgccagct caacagcggc
    70861 ttccagcaaa atggagtgcg gaacaacgtc atccaccagc cccagcttta atgcctgttt
    70921 cgcccgaagt tgttttccgg tgagaatcat ctctaatgct gtactgacgc ctatcagacg
    70981 cggtaaacgc tgggtgccgc ctgaaccggg taacaatcca agttgtactt caggcaaacc
    71041 gagcaccgtt ttaggatcgt cagtacaaac gcgaccgtgg cacgccagcg ccagctccag
    71101 cccaccgccc aggcaagcgc catgaatagc cgcgataacc ggaatgggca aagcatgaat
    71161 ctccgccatc aactgttggc cctgccgcgc cagtgcttcc gcttcttgcg ccgttttgca
    71221 gttgccaatc atgttgatgt ctgctccggc gataaaattg tccggtttcg cggagataaa
    71281 caccacgccg cgcaactctt tgttttcacg gagttgctta ataatggcgc gcacctgcga
    71341 ggcaaattcc gccttcaggg tattcatttt ctcatccggt acgtcgatgg tgatgatagc
    71401 aatgttgtcc agacgaacat taagagtaaa cgctgatgcc atttccatta ttccgcctcc
    71461 agaaccattg ccgcgccaag cccaccggca gcacaggcgg taactaaacc aaatccaccg
    71521 ccgcgacggc gaagttcatg caatgtctgg gtaatcatcc gcgcgccggt cgccgcgaag
    71581 ggatgtccgt aagcaatcga accgccaagc acgttaaatt tgctatcgtc cacttcgcca
    71641 gtggcatgtg cacgccccag tacttcacga gcaaaacgtt cactacccag caactgaata
    71701 ttcgccagtg tctgagctgc aaaggcttcg tgcatatcga tcaatgtcag ttcgctcatc
    71761 gtcaaaccgg cacgttccag cgccagcggt gttgaccagg ctggaccgag caacatgtcc
    71821 tgccagacat caatcgcagt aaatgcgtag ctgcgcagat accccagcgg caccagccct
    71881 aattctttcg cccgggattc agtcatcagg atcaccgctg ccgcgccatc ggtcagcggc
    71941 gtactgtttg ccgccgttac cgttccgtgt ttgcgatcaa acgccgggcg cagctttgcg
    72001 taatcggcaa gcgaggaatt accgcgaata ttgttgtctt cgacaagcgg ttgtttataa
    72061 ggagggataa aggcagtcat cacctcttct ttgagttttc cgtctgacca tgcctgagcg
    72121 gcacgctgat gcgaacggtg cgctaatgca tcttgctgtt ctcgggtgat gccgtaggtt
    72181 ttcgccattt gttcggcggt gtcgcccatc cgcaagccag tagaatattc tgctaccgca
    72241 ggcggtacgg gcattaagtc gcgcaaacgc aggcgagaga agagtttcag gcgctggctc
    72301 atggttcgag ctttgttgac atcaaccagc acgtgcgcca gttttttact gacgccaatt
    72361 ggcaatacgg aagaggaatc tgccccaccg gcaatccccg ctcggatagt tcccgccatc
    72421 aggctttctg cgacgtttgc aaccgcctgg aaactggtag cgcaagcgcg gctgacgctg
    72481 taagcatcgg tatgcacatt cattccggta ccgagaacaa tttcacgcgc aatgttgggg
    72541 gcttcaggca tttgtacgac ctgaccaaag accagctgtt caatcacttc agcggggatc
    72601 tcgctgcgtg ccagcagttc gcctaccacc atcttcccta aatcaaccgc gggaatgcca
    72661 tgaaaagccg tcgcctggcg ggcaaaaggc gtacgtaaac cgctaacaat ggcgatacga
    72721 tcgccctggc gggtaaccag tggtaaaacc tgacccataa cactcccctg taaaaaaata
    72781 aataaagtgg tctgacctga tcatagtatt aaccattttt ttacatttag ccaagcggag
    72841 aaaaaggaaa gtgggagcta tgacacagtg aagacagaag aaagaaaaga aaacgcccct
    72901 gccagcaact gacaggggcg tacgtacccg agaggaatta acgcagaccc agctggaaga
    72961 tcagcatttc agcttcgcag gcaaaagtga aatcgatatc caggcgcaca ccgtcagact
    73021 cttcagtgaa agtcggggag attttgcacg gttcggattc cacgctacgg gatttttcag
    73081 tcagcgccgc cagcgtttgt tctgcttctg cgcggtttgc aaacacgcgg ctgtaagacg
    73141 cggtgcagtc ggagttgtcc ataatggtgc caacatccat acagcagcaa accggggttt
    73201 catcagcact acatttactc atcgttgatt tcctctgtat gtgcacccaa ggtgccagat
    73261 aaacgttgtg gatattttac gcttccggaa agtgctgctc cagttgttaa ttctgcaaaa
    73321 tcggataagt gaccgaaatc acacttaaaa atgatctaaa acaaaattca cccgaatccg
    73381 cgagtgcgca acctccaaat tttgccagct ggatcgcgtt tcttagatca tatttgaaaa
    73441 aagatggaaa catacttgca acattctagc tggtccgacc tatactctcg ccactggtct
    73501 gatttctaag atgtacctca gaccctacac ttcgcgctcc tgttacagca cgtaacatag
    73561 tttgtataaa aataaatcat tgaggttatg gtcatgagcc agaaaaccct gtttacaaag
    73621 tctgctctcg cagtcgcagt ggcacttatc tccacccagg cctggtcggc aggctttcag
    73681 ttaaacgaat tttcttcctc tggcctgggc cgggcttatt caggggaagg cgcaattgcc
    73741 gatgatgcag gtaacgttag ccgtaacccc gcattgatta ccatgtttga ccgcccgaca
    73801 ttttctgcgg gtgcggttta tattgacccg gatgtgaata tcagcggaac gtctccgtct
    73861 ggtcgtagcc tgcaagccga taacatcgcg cctacggcat gggttccgaa catgcacttt
    73921 gttgcaccga ttaacgacca atttggttgg ggcgcttcta ttacctctaa ctatggtctg
    73981 gcaacagagt ttaatgatac ttatgcaggc ggctctgtcg ggggtacaac tgaccttgaa
    74041 accatgaacc tgaacttaag cggtgcgtat cgcttaaata atgcatggag ctttggtctt
    74101 ggtttcaacg ccatctacgc tcgcgcgaaa attgaacgtt tcgcaggcga tctggggcag
    74161 ttggttgctg gtcagattat gcaatctcct gccgggcaaa ctcctcaagg gcaagcattg
    74221 gcagctaccg ccaacggtat cgacagtaat accaaaatcg ctcatctgaa cggtaaccag
    74281 tggggctttg gctggaacgc cggaatcctg tatgaactgg ataaaaataa ccgctatgca
    74341 ctgacctacc gttctgaagt gaaaattgac ttcaaaggta actacagcag cgatcttaat
    74401 cgtgcgttta ataactacgg tttgccaatt cctaccgcga caggtggcgc aacgcaatcg
    74461 ggttatctga cgctgaacct gcctgaaatg tgggaagtgt caggttataa ccgtgttgat
    74521 ccacagtggg cgattcacta tagcctggct tacaccagct ggagtcagtt ccagcagctg
    74581 aaagcgacct caaccagtgg cgacacgctg ttccagaaac atgaaggctt taaagatgct
    74641 taccgcatcg cgttgggtac cacttattac tacgatgata actggacctt ccgtaccggt
    74701 atcgcctttg atgacagccc agttccggct cagaatcgtt ctatctccat tccggaccag
    74761 gaccgtttct ggctgagtgc aggtacgact tacgcattta ataaagatgc ttcagtcgac
    74821 gttggtgttt cttatatgca cggtcagagc gtgaaaatta acgaaggccc ataccagttc
    74881 gagtctgaag gtaaagcctg gctgttcggt actaacttta actacgcgtt ctgataacgc
    74941 gttcgcctgg ataaagtcac ctgctatgca ggtgacttta actcccccac ttcaccgaaa
    75001 gtagtttcct cattgcttgc ctcctgagtt ttgcaaacag cctgatggca gcttgccctt
    75061 tcaaatcagt aagtggtatc cacagacaaa cctgaaggga aatgcatatt ttcaagcgtt
    75121 ctgctcgtcc ttctcaaaga gtttactttt ctgcatttcc aggatactca acccccctgg
    75181 ctattgtgcg cttatacact caaattaaag ataggttcta aataaatgag cgttttttga
    75241 tagtctattt cattaggtaa tatatatttg taacaaatca atcaacatag aataaaatca
    75301 tgctaccatc tatttcaatc aacaatacca gcgcagctta cccagaatcc atcaatgaaa
    75361 ataacaatga tgaaattaat ggattagtac aagagttcaa aaaccttttt aatggtaagg
    75421 aaggaataag cacctgtatt aaacatctac ttgagcttat aaaaaacgcc atacgagtaa
    75481 acgacgatcc ttatagattt aatattaata attcctcagt tacttatatt gatattggct
    75541 ccaatgatac agaccatatt actattggta tcgacaacca atagaattac ctgcgaacta
    75601 taaagacaaa gaactcgtcc gtactatcat taatgacaac atagttgaga agactcatga
    75661 tatcaataac aaggaaatga tcttcagcgc attaaaagaa atatatgatg gagatcctgg
    75721 ttttatcttc gataagatat cacacaaact cagacatacg gtaacggaat ttgatgagag
    75781 cgggaaaagc gaaccaacgg acttatttac ctggtacggt aaagataaaa aaggcgactc
    75841 tctcgctatt gtaattaaaa ataaaaacgg aaatgattac ttatctctcg gttactacga
    75901 tcaggacgac taccacattc aaagaggaat tcgtattaat ggtgatagtc tcacccaata
    75961 ttgtagtgaa aacgccagga gtgcttcagc gtggtttgaa agcagtaaag ctatcatggc
    76021 agaatcattt gcaactggtt ccgatcatca ggttgtaaac gagctcaacg gggaaagact
    76081 gagagaacct aacgaagttt ttaaacgttt aggtcgagca ataagatata attttcaagt
    76141 ggacgatgca aaatttagac gcgataatgt aaaagaaata atttctactt tattcgctaa
    76201 caaagttgac gttgaccatc ctgaaaataa atacaaagac tttaaggatc tcgaagataa
    76261 agttgaaaaa agacttcaaa attgccaggc gaaatatcaa aatgaaatta atcaactatc
    76321 tgcactaggt gttaattttg atgatattta aaattatttt tccagttttt tgatttttca
    76381 acgcctgaat atcggaaata gtcagtgaca aac
//
LOCUS       DAETPZ010000005        71610 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.005, whole genome shotgun sequence.
ACCESSION   DAETPZ010000005 DAETPZ010000000
VERSION     DAETPZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 71610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71610
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.005"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..413)
                     /locus_tag="K0W59_000419"
     CDS             complement(<1..413)
                     /locus_tag="K0W59_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBI3021592.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
     gene            complement(410..844)
                     /locus_tag="K0W59_000420"
     CDS             complement(410..844)
                     /locus_tag="K0W59_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBI3021593.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEEYPVEEGI"
     gene            complement(931..1740)
                     /locus_tag="K0W59_000421"
     CDS             complement(931..1740)
                     /locus_tag="K0W59_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBI3021594.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            complement(1730..2509)
                     /locus_tag="K0W59_000422"
     CDS             complement(1730..2509)
                     /locus_tag="K0W59_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HBI3021595.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGTEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            complement(2520..2984)
                     /gene="agaV"
                     /locus_tag="K0W59_000423"
     CDS             complement(2520..2984)
                     /gene="agaV"
                     /locus_tag="K0W59_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HBI3021596.1"
                     /translation="MVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQQNL
                     MEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRINVG
                     NMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            complement(2984..3784)
                     /locus_tag="K0W59_000424"
                     /pseudo
     CDS             complement(2984..3784)
                     /locus_tag="K0W59_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
     gene            4033..4857
                     /gene="agaR"
                     /locus_tag="K0W59_000425"
     CDS             4033..4857
                     /gene="agaR"
                     /locus_tag="K0W59_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HBI3021597.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            complement(4909..6480)
                     /gene="garD"
                     /locus_tag="K0W59_000426"
     CDS             complement(4909..6480)
                     /gene="garD"
                     /locus_tag="K0W59_000426"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HBI3021598.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPTSELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6855..8189
                     /gene="garP"
                     /locus_tag="K0W59_000427"
     CDS             6855..8189
                     /gene="garP"
                     /locus_tag="K0W59_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HBI3021599.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDSAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            8205..8975
                     /gene="garL"
                     /locus_tag="K0W59_000428"
     CDS             8205..8975
                     /gene="garL"
                     /locus_tag="K0W59_000428"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HBI3021600.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATHKLADTFKK"
     gene            9005..9895
                     /gene="garR"
                     /locus_tag="K0W59_000429"
     CDS             9005..9895
                     /gene="garR"
                     /locus_tag="K0W59_000429"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBI3021601.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            9992..11143
                     /gene="garK"
                     /locus_tag="K0W59_000430"
     CDS             9992..11143
                     /gene="garK"
                     /locus_tag="K0W59_000430"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HBI3021602.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGAGSLTDDVGV
                     VHQHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            11176..11552
                     /gene="rnpB"
                     /locus_tag="K0W59_000431"
     ncRNA           11176..11552
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="K0W59_000431"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(12173..13339)
                     /locus_tag="K0W59_000432"
     CDS             complement(12173..13339)
                     /locus_tag="K0W59_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
                     /protein_id="HBI3021603.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLKNQEISTGNINVNCPLKDIYNEIRRLNVIFSCGTGDIVDLSSLDLRN
                     VDLDYYDFTDKHMANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKITPV
                     LISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELTKE
                     IYKSSSKINNCLNRIKLPEMKEFSSKKIYDYIDTIIEDYENIKENAYLVVPQINYTMD
                     LNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
     gene            complement(13361..13900)
                     /locus_tag="K0W59_000433"
     CDS             complement(13361..13900)
                     /locus_tag="K0W59_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3021604.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(14156..14500)
                     /gene="tdcR"
                     /locus_tag="K0W59_000434"
     CDS             complement(14156..14500)
                     /gene="tdcR"
                     /locus_tag="K0W59_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HBI3021605.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            14689..15627
                     /gene="tdcA"
                     /locus_tag="K0W59_000435"
     CDS             14689..15627
                     /gene="tdcA"
                     /locus_tag="K0W59_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HBI3021606.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            15726..16715
                     /gene="tdcB"
                     /locus_tag="K0W59_000436"
     CDS             15726..16715
                     /gene="tdcB"
                     /locus_tag="K0W59_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HBI3021607.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            16737..18068
                     /gene="tdcC"
                     /locus_tag="K0W59_000437"
     CDS             16737..18068
                     /gene="tdcC"
                     /locus_tag="K0W59_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HBI3021608.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKVQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            18094..19302
                     /gene="tdcD"
                     /locus_tag="K0W59_000438"
     CDS             18094..19302
                     /gene="tdcD"
                     /locus_tag="K0W59_000438"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HBI3021609.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLALHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWIASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            19336..21629
                     /gene="tdcE"
                     /locus_tag="K0W59_000439"
                     /pseudo
     CDS             19336..21629
                     /gene="tdcE"
                     /locus_tag="K0W59_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
     gene            21643..22032
                     /locus_tag="K0W59_000440"
     CDS             21643..22032
                     /locus_tag="K0W59_000440"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HBI3021610.1"
                     /translation="MKKIIETQRALGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            22104..23468
                     /gene="tdcG"
                     /locus_tag="K0W59_000441"
     CDS             22104..23468
                     /gene="tdcG"
                     /locus_tag="K0W59_000441"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBI3021611.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCD"
     gene            23743..25020
                     /locus_tag="K0W59_000442"
     CDS             23743..25020
                     /locus_tag="K0W59_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3021612.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YSVSFTLAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFGVYLGFREATQGIVMNILR
                     RKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTSICSPIFGMVGCLIPAWLV
                     YKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            25048..26340
                     /locus_tag="K0W59_000443"
     CDS             25048..26340
                     /locus_tag="K0W59_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBI3021613.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGITVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGAKVSTSASAAWKAVLMALDDTAVTGNE
                     GIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(26552..26716)
                     /locus_tag="K0W59_000444"
     CDS             complement(26552..26716)
                     /locus_tag="K0W59_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3021614.1"
                     /translation="MSKKSTKKRQLAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(26739..27440)
                     /locus_tag="K0W59_000445"
     CDS             complement(26739..27440)
                     /locus_tag="K0W59_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HBI3021615.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            27545..28441
                     /gene="yhaJ"
                     /locus_tag="K0W59_000446"
     CDS             27545..28441
                     /gene="yhaJ"
                     /locus_tag="K0W59_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HBI3021616.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYIMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGQLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            complement(28648..29016)
                     /locus_tag="K0W59_000447"
     CDS             complement(28648..29016)
                     /locus_tag="K0W59_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBI3021617.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(29309..30295)
                     /gene="yqjG"
                     /locus_tag="K0W59_000448"
     CDS             complement(29309..30295)
                     /gene="yqjG"
                     /locus_tag="K0W59_000448"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HBI3021618.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(30365..30757)
                     /locus_tag="K0W59_000449"
     CDS             complement(30365..30757)
                     /locus_tag="K0W59_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBI3021619.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFSFLTRTTTLFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(30943..31242)
                     /locus_tag="K0W59_000450"
     CDS             complement(30943..31242)
                     /locus_tag="K0W59_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HBI3021620.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(31232..31636)
                     /locus_tag="K0W59_000451"
     CDS             complement(31232..31636)
                     /locus_tag="K0W59_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBI3021621.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(31639..31944)
                     /locus_tag="K0W59_000452"
     CDS             complement(31639..31944)
                     /locus_tag="K0W59_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBI3021622.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(31982..32336)
                     /locus_tag="K0W59_000453"
                     /pseudo
     CDS             complement(31982..32336)
                     /locus_tag="K0W59_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            complement(32483..32866)
                     /gene="mzrA"
                     /locus_tag="K0W59_000454"
     CDS             complement(32483..32866)
                     /gene="mzrA"
                     /locus_tag="K0W59_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HBI3021623.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(32870..33532)
                     /locus_tag="K0W59_000455"
     CDS             complement(32870..33532)
                     /locus_tag="K0W59_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI3021624.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(33877..34653)
                     /gene="exuR"
                     /locus_tag="K0W59_000456"
     CDS             complement(33877..34653)
                     /gene="exuR"
                     /locus_tag="K0W59_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HBI3021625.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            34949..35665
                     /locus_tag="K0W59_000457"
     CDS             34949..35665
                     /locus_tag="K0W59_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBI3021626.1"
                     /translation="MVAKINLLLRLTPLLFVFAPFLAQSNMTVYPMPVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNSAQKLINAGNQRLKLLTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            35695..36195
                     /locus_tag="K0W59_000458"
     CDS             35695..36195
                     /locus_tag="K0W59_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3021627.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            36287..38796
                     /locus_tag="K0W59_000459"
                     /pseudo
     CDS             36287..38796
                     /locus_tag="K0W59_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
     gene            38793..39881
                     /locus_tag="K0W59_000460"
     CDS             38793..39881
                     /locus_tag="K0W59_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HBI3021628.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(39969..41267)
                     /gene="exuT"
                     /locus_tag="K0W59_000461"
     CDS             complement(39969..41267)
                     /gene="exuT"
                     /locus_tag="K0W59_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HBI3021629.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            41750..43162
                     /gene="uxaC"
                     /locus_tag="K0W59_000462"
     CDS             41750..43162
                     /gene="uxaC"
                     /locus_tag="K0W59_000462"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBI3021630.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSSRGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            43177..44664
                     /gene="uxaA"
                     /locus_tag="K0W59_000463"
     CDS             43177..44664
                     /gene="uxaA"
                     /locus_tag="K0W59_000463"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBI3021631.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            44747..45298
                     /locus_tag="K0W59_000464"
     CDS             44747..45298
                     /locus_tag="K0W59_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HBI3021632.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(45303..46547)
                     /gene="sstT"
                     /locus_tag="K0W59_000465"
     CDS             complement(45303..46547)
                     /gene="sstT"
                     /locus_tag="K0W59_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBI3021633.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(47143..48108)
                     /gene="alx"
                     /locus_tag="K0W59_000466"
     CDS             complement(47143..48108)
                     /gene="alx"
                     /locus_tag="K0W59_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HBI3021634.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(48379..49377)
                     /locus_tag="K0W59_000467"
     CDS             complement(48379..49377)
                     /locus_tag="K0W59_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBI3021635.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(49456..50148)
                     /locus_tag="K0W59_000468"
     CDS             complement(49456..50148)
                     /locus_tag="K0W59_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HBI3021636.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(50225..50728)
                     /locus_tag="K0W59_000469"
     CDS             complement(50225..50728)
                     /locus_tag="K0W59_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBI3021637.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRMQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            50813..51949
                     /gene="rlmG"
                     /locus_tag="K0W59_000470"
     CDS             50813..51949
                     /gene="rlmG"
                     /locus_tag="K0W59_000470"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HBI3021638.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            52234..52548
                     /gene="higB"
                     /locus_tag="K0W59_000471"
     CDS             52234..52548
                     /gene="higB"
                     /locus_tag="K0W59_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HBI3021639.1"
                     /translation="MHLITQKALKDAAEKHPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            52545..52961
                     /gene="higA"
                     /locus_tag="K0W59_000472"
     CDS             52545..52961
                     /gene="higA"
                     /locus_tag="K0W59_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HBI3021640.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(53006..55024)
                     /gene="fadH"
                     /locus_tag="K0W59_000473"
     CDS             complement(53006..55024)
                     /gene="fadH"
                     /locus_tag="K0W59_000473"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HBI3021641.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLIGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLQLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(55250..57601)
                     /gene="ygjK"
                     /locus_tag="K0W59_000474"
     CDS             complement(55250..57601)
                     /gene="ygjK"
                     /locus_tag="K0W59_000474"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBI3021642.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(57618..58037)
                     /locus_tag="K0W59_000475"
     CDS             complement(57618..58037)
                     /locus_tag="K0W59_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3021643.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(58015..58368)
                     /locus_tag="K0W59_000476"
     CDS             complement(58015..58368)
                     /locus_tag="K0W59_000476"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3021644.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(58369..58689)
                     /locus_tag="K0W59_000477"
     CDS             complement(58369..58689)
                     /locus_tag="K0W59_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3021645.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGMEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(58823..60256)
                     /locus_tag="K0W59_000478"
     CDS             complement(58823..60256)
                     /locus_tag="K0W59_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI3021646.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGDVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(60319..60768)
                     /locus_tag="K0W59_000479"
     CDS             complement(60319..60768)
                     /locus_tag="K0W59_000479"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HBI3021647.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(60765..63851)
                     /gene="ebgA"
                     /locus_tag="K0W59_000480"
     CDS             complement(60765..63851)
                     /gene="ebgA"
                     /locus_tag="K0W59_000480"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HBI3021648.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAYYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIINRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHDNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(64033..65016)
                     /gene="ebgR"
                     /locus_tag="K0W59_000481"
     CDS             complement(64033..65016)
                     /gene="ebgR"
                     /locus_tag="K0W59_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HBI3021649.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELNN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            65235..65566
                     /locus_tag="K0W59_000482"
                     /pseudo
     CDS             65235..65566
                     /locus_tag="K0W59_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(65608..66939)
                     /gene="ygjG"
                     /locus_tag="K0W59_000483"
     CDS             complement(65608..66939)
                     /gene="ygjG"
                     /locus_tag="K0W59_000483"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HBI3021650.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEM
                     FRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            67356..68876
                     /gene="aer"
                     /locus_tag="K0W59_000484"
     CDS             67356..68876
                     /gene="aer"
                     /locus_tag="K0W59_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HBI3021651.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMFTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNSGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRTRGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(69030..69653)
                     /gene="nfeR"
                     /locus_tag="K0W59_000485"
                     /gene_synonym="yqjI"
     CDS             complement(69030..69653)
                     /gene="nfeR"
                     /locus_tag="K0W59_000485"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HBI3021652.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            69930..70676
                     /gene="nfeF"
                     /locus_tag="K0W59_000486"
                     /pseudo
     CDS             69930..70676
                     /gene="nfeF"
                     /locus_tag="K0W59_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(70730..70805)
                     /locus_tag="K0W59_000487"
     tRNA            complement(70730..70805)
                     /locus_tag="K0W59_000487"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(70770..70772),aa:Ile,seq:cat)
     gene            70930..71436
                     /gene="mug"
                     /locus_tag="K0W59_000488"
     CDS             70930..71436
                     /gene="mug"
                     /locus_tag="K0W59_000488"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HBI3021653.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(71470..>71610)
                     /locus_tag="K0W59_000489"
     CDS             complement(71470..>71610)
                     /locus_tag="K0W59_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBI3021654.1"
                     /translation="PELMRDLASMGQLVKLPTRRGRAFPRVVKERPWKYPTAPKKSQS
                     VA"
BASE COUNT        17651 a        17637 c        18507 g        17815 t
ORIGIN      
        1 ggatgcgcgc ctttattctg cggcgtgaag tacggtcctt cgagataact ccccagcact
       61 tgcgcaccag gtccgccgcg ttggcaacgt tgagcaatac gtttcagcgc cgcatgaatg
      121 gtattaagcg gcgcggttac ggtggtcggt aaccaactgc cgacaccttc gcgtgccttg
      181 tgcattgcca gcttgtcgag tacatccggc gcgtcatcca ttacatcaac gcccgcacca
      241 ccgtgtacat gggtgtcgat gtaagcgggg cagagcagtt ccgcatcgcg ttcagtcacg
      301 cccactggaa tcggttcgat tgctgcgatg acaccgtcag caacacgcaa ctgatggtca
      361 tcgaaccatc cctcttcagt cagcagcctt ctggcgcgca gaacgtgtgt catattcctt
      421 cctcgaccgg atattcttca tggcagcggc ctaactcgtc caccagactg gtcagccctc
      481 ggtgtccaca ctccagcgcc tgtacacgga attcttcacc gcttaaccct tcgcgctcca
      541 gcaccatctc cagtagcagt tgcaaattgg tgccggtaat cacttcacag cccggttttt
      601 gcatcgccag cgttgaagct acgcgaaacg gcgtgccgcc cagcaaatcc gtcagaaaaa
      661 caatgccgtc ttcacaatcc agttgggcga tggcctcttc aagctgcgat gtaagcagcg
      721 cggcgcttga ggtttcggga aaatcgatgg caataaactg cgattgatcg ccgaggattt
      781 gcttcattgc tttttccatg ccactggcaa acccgccatg ccctgtcaaa ataatactta
      841 acatctaata cctcattgta tttaaaggaa atggcagaat ttaccgacga tccctagaac
      901 cacggtaacc gcaatcaggc gcagcggact ctagccgcgg cgtaccagcc agaacatggt
      961 cagggtgtag accagcggta agaaggcagg catcagtttg tcgataacat cagcctgtaa
     1021 cttaaccact gcgtcgcccg ccgtgatttc cagcgtggtg cttaaacgaa cataggttgc
     1081 caccagtgcg ccgattaccg tcattccaac gattgacgca gcatggccga cttttttggt
     1141 attcgcttta ataaggggaa tcgccgccac gcccatgcgg taagcataat gcgccagacc
     1201 aaaacgcagc cccaagtgta ccacgttgaa cagcacaata aagacgactg cgccaagaat
     1261 agagccttgt agcgcgaggc ttgcgcctat cccgccacaa atcggcagta gcgttagcca
     1321 gaacatcgca tcgccaatcc cgccaagcgg cgcaccgacg gcaattttgg tgctctgaat
     1381 actgttaacg tcctgcttag aacgctccat cgcaaggata atgccgataa caaacgtcac
     1441 cagaaacgga tgggtattga agaaacccat atgccccttc atggcgcgcg ccaggtcgcg
     1501 tttattagtg tggatctttt tcagagcagg cagcagaccg tacaaccagc cagaagcctg
     1561 catacgttcg tagttaaaag acgcttgtaa cagcatggaa cgccaggcga cgcggttgat
     1621 atcttttttc gtcagctccg cggcaatgct ccgatcttca tacacgtttt cattgacgcc
     1681 agtcagcagt gtttcttcat tttcgctgac gttcggcagg gtggtttgat tagatgccat
     1741 cttcaaattc ctctttctgt gccgcaggtt gagtcggttc aggagattta cgcagcagat
     1801 cgatcagcgc catcgccagc gcggcggcag caatagccag caccggtaac ttgagccagg
     1861 ctgcggcaac aaagcccagg atgaagtagg gtatgtagac gtttttcatc atgattttca
     1921 gcagcacggc aaaaccgatt gctggcatga tgccgcccgc gacgccgagg ccgtcgatta
     1981 atcgttgcgg caggacatca atgatggttt ttgcatgttc cgtgccgaag tagataggca
     2041 ggaaagcgca gagaaaatag aaagtgccga gtgccagcag tgccagatag ttcacgcgtt
     2101 caatgccgcg agtatcggcg ttctccgcca tccggtcgca gcgagacatc acgccagaca
     2161 tcaccgagaa caggaaggta atccccatct gtactgcgac agcgaaaggt acggcgacac
     2221 cgaccgcgac atcgggtttc acgccagtag taatggcaaa cgccgtgccg acgatagtac
     2281 cgataatcac gttaggcggc tgtgcgcccg ccagcggggc cagccccatc cacactagtt
     2341 ccagcgtacc gccggttaaa attccggtat gcagatcgcc aagtaccagc ccgaccaacg
     2401 ggccgaggac caccgggcgg tgcatatggg ttaagccgtt aaacatatct aggccagcga
     2461 taaaggcgat aatgcccaac gcaaatgcct gcaacaggct gatttccatg atgaatccct
     2521 caaagtaatt taaagaggtc cacagcaggc tctgtcggga cgccctgaac gaagcattcc
     2581 accccagcgg ctttcaggtc gttaaatgct gcgatatcgc ccgcatccac agaaaccgtt
     2641 ttggcgattt gttgtttgcc attggcgtag tgcatattgc caacgttaat gcgattcacc
     2701 ggaacgccac ctttcaccag cgtcaggaaa tcggcgggtg ttttacaaac cagcaggatt
     2761 ttctgtcgat cggcggcgcg atgaatgttg tcgataactt tttgcagcgt ccagaaacgt
     2821 acggcgatcc cttctgccag taccatttcc atcaggtttt gttgtaccgg atcttcggca
     2881 acctcatcgt tggctaccag caccagattt gcccccgcaa atccgaccca ctgaacgccg
     2941 acctgaccgt gaatcaagcg ttcatcaatc cggcttaaaa caattgataa tcggtagagt
     3001 gcgcttcgaa aatcagcgtt tcgtagtttt cgaccatctg gcttaaggcg ctcgctttgg
     3061 cgggctgata atcaataacg ttggtgtgat cgaattcgac gccgggttga accaccaggg
     3121 caatgatgcg tggccagatg gcattcaaac cttgcttttc aaaggcgtga cgatgggctt
     3181 ccagcgtggc gcgggcggca tccggcgtgg tgaccgccag ctcgcttaag gtttcatgcg
     3241 cgccgccagg taccggcact tcggtaccaa tgacatactc cagatcggct tcgccaaagt
     3301 gttcgcgaca ggtttcttcc gccactttcg ccagacgggc ggcgcgttca gccacgatgt
     3361 catcagttaa gggaattgga tcgtcctgac aggacatgct gcaatcaagg tggatttttt
     3421 tgaatcctgc cgcaacgtag cttttaatca aatcatcggc attggccatt gcctgagcgg
     3481 ctggcaggtt ttgccagcgg tttggcccca gatggtcacc acccagaatc agcgcatcct
     3541 gcgggaaatt caacgagtcg gcgagctgac aaacaaagcc gcgaaaatcg gcgggcgtca
     3601 ttccggtata accgccgaac tggtctacct gattggaggt tgcttcaatc agtaacggcg
     3661 tttggtttgc actggcgtag cggattgcag cctccagcac cagcggatgt gcggaacaaa
     3721 cggcataaat tgcatttgtt ttgcccgctt tgtgctgtct caccatttct gtcagatgtt
     3781 tcactttcct ctccagttca gattactgcg atattaatct ttcgttttgt ttcaattgat
     3841 actgcatcat ggtgagataa aaataaaacg aaatataata gttttctgac ctgattcaca
     3901 aaagaaacat aatgaaagtc actgaaacga gagtagcttg cgccaatggg caagatgggc
     3961 ttgcatttgc ttaatagaaa ggcgttaata ggcaaaacga aatgaaacga aagttttacg
     4021 aaaggactta ctatgagtaa taccgacgct tcaggtgaga agcgagtgac aggcaccagc
     4081 gagcgacgag aacagatcat tcagcgtctg cgacagcaag ggagtgtgca ggttaacgat
     4141 ctgtcggcat tgtatggcgt atctactgtg acgatccgca atgatctggc ttttctggaa
     4201 aagcagggga tcgctgtgcg tgcctatggt ggcgcgttga tctgcgatag cacgacgccg
     4261 tcagtcgagc catcagtgga agataaaagc gcactgaaca ccgcgatgaa acgcagcgtt
     4321 gcgaaagctg ccgttgagtt gattcagcca ggtcatcggg tgatcctcga ttccgggacc
     4381 accacttttg agattgctcg tctgatgcgc aagtacactg acgtaattgc gatgaccaac
     4441 ggtatgaacg tggctaatgc gttgctggaa gcggaaggcg ttgagctgct gatgaccggc
     4501 gggcatttgc gccgtcagtc gcaatctttt tacggcgatc aggctgagca atcgctgcaa
     4561 aattaccact tcgatatgct gtttcttggt gtagatgcga tcgatctgga gcgcggcgtc
     4621 agcacacata atgaagatga agcccgttta aaccgtcgga tgtgcgaagt tgcggaacgg
     4681 atcatcgtgg tcaccgattc cagtaaattc aatcgttcga gtttacataa gatcattgat
     4741 actcaacgta tcgacatgat cattgttgat gaaggcattc ctgcggatag tctggaagga
     4801 ctgcgaaagg ctggggttga agtgattctg gtcggggatc acgcgtcttc gctgtgactc
     4861 aatatgcccg ataaacgtgt gctatcgggc ataatacgaa ttgaattatc aggtcaccgg
     4921 tgctgggtta aacaccgcca gctggttatg tagtccccat tgatccgaga aggttttctt
     4981 cttcccgctg gcgacgtcga gaataaagtg gaacaacttc cagcccacct cttcaatagt
     5041 ttcttcgccg gtagcgatgg tgcccgcatt aatatccatt aaatcaaacc agcggttcgc
     5101 cagctcggtg cgggttgcca ttttaatgac gggtaccgcc atcaggccgt acggcgtacc
     5161 acgaccggtc gtaaacactt gcacggtgat acccgaagcc acctgttgcg tgccacagac
     5221 aaaatcgctg gctggcgtcg cggcgtaaat taatccgcgt ttagtcgggc gttgaccggg
     5281 cgacagcact tcaacaattg cacttttgcc cgatttagca atggagccga gcgctttctc
     5341 taccacgttt gccagaccgc ctttcttgtt gcccggcgaa gggttggcgc tgcggtcggt
     5401 ttttcccata ttgagatagt tatcgtacca ctccatctcc tccagcagcc gtttgccgac
     5461 ctcctcgttc actgcgcgtg gtgtcagcag atggatcgcg tcacgcactt ccgtgacttc
     5521 tgaaaacatc accgttgcgc cgcagcgcac cagtagatca gacgcatagc caaccgccgg
     5581 gtttgccgtt acgccagaaa acgcatcgct gccaccgcac tgcatgccga caaccagttc
     5641 tgaagtcgga caggtttctc gctgacgttg attcagtttt tgtagatggc gttcggcgac
     5701 ttgcaaaata tcctcgacca tggactgaaa accgacatgc ttttcatcct gcaaactgac
     5761 aatgctggcg ctttctactg gaatagcttg cacatcatcc gttccagtca gcaggcgctc
     5821 aggctgcaac ttttcacaac ccaggccaat caccattact tcgccgccaa agttagggtt
     5881 cagcgaaata ttgtgaatgg tacgaatagg tacaactgcc gccggtgcgt taatcgccac
     5941 gccacaaccg tacaaatgat tcagccccac cacgccatcg acgttcgggt atttcggtag
     6001 cagatcgcgt tcaatgattt ttactacata gtccaccacg cctgccacac agtggacgct
     6061 ggtggtgatg ccgagcaggt ttttggtgcc cacgctgcca tcggcattgc gatagccctc
     6121 aaaggtgtat ccttccagcg gcggtaaggg ttccgggact ttggttgcca gtggcagcgt
     6181 gtgtaacggc ggcgcttccg gtagtacaac cattgattcg tcgatccagc ttccgcgtgg
     6241 gattgcacgc acggcgtaac caatcacttc gccataacga ataatttcac cattagccgg
     6301 aatgtccagc aatgcgactt tatgcccctg gggaatatgt tcaattaatt ccagcccatc
     6361 cggaaaacgc gttcctgctt tcaggccatt atcattaaca ataattgcca cattatctgt
     6421 gtcgtgaact tttatataaa acgcagttgg cgtttcttgt ctgatttcga tgttggccat
     6481 tgtgcaatat tctccagcca ggggttatta aaatgaggac ctggtctcat ttaaaaataa
     6541 atgacttcat tgggtgaaat aagaatgtca ggagtttaaa cgcagaaatg tgatcaatgt
     6601 caatcatttc agggattgca gtgccgcccg gcaaggtaaa gcgcaaatct gtgctttagc
     6661 gcgcaacctt atgtgcatat gctcaaaata atatttaaaa agtattaatt aaattagcat
     6721 ttgcacaatg attattattc tcgcgctgct tatactaatt cccgagttca ttgtcgttac
     6781 ggcataaatg aaacttataa ataacacctt ccccataata ataaaaatca gcataagtac
     6841 ccgaggtaaa taaaatgatt ctggacaccg ttgacgaaaa aaggaaaggt gtgcataccc
     6901 gctatttaat attactgatt atttttattg ttaccgccgt taactacgcc gatcgtgcaa
     6961 cgctgtctat tgctggtacc gaagtggcaa aagagttgca gttaagtgcg gtttcgatgg
     7021 gttacatctt ctccgctttt ggctgggcct acttgctgat gcaaatcccc ggcggctggt
     7081 tgcttgataa gtttggctcg aaaaaagttt acacctacag cctctttttc tggtcgctgt
     7141 tcaccttcct gcaaggcttt gttgatatgt tcccgctggc ctgggcaggg atctccatgt
     7201 tctttatgcg ctttatgctg ggattctcgg aagctccatc attcccggcg aacgcccgta
     7261 ttgtcgccgc ctggttcccg acgaaagaac gtggtactgc ctccgccatc tttaactcgg
     7321 cacaatattt ctcgctggcg ctcttttcgc ctctgcttgg ctggctgact ttcgcctggg
     7381 gatgggagca cgtctttacc gttatggggg tgattggttt tgtgctgacg gcgctgtgga
     7441 tcaagttgat tcataacccg acagatcacc cacgtatgtc tgaggaagag ctgaagttta
     7501 tctctgaaaa tggcgcggtg gtcgatatgg accacaaaaa gccgggcagc gcggcagcaa
     7561 gcggtcccaa actgcattac atcaagcaat tgctctctaa ccgcatgatg ttgggcgtat
     7621 ttttcggaca atattttatc aacaccatca cctggttctt cctcacctgg ttcccgattt
     7681 atctggtgca ggaaaaaggc atgtcgattc tgaaagtggg tctggtcgcc tcgattccgg
     7741 cactgtgtgg ttttgcgggc ggcgtgctgg gaggtgtctt ctcggattat ctgatcaaac
     7801 gcggtttatc cctgaccctg gcacgtaagc taccgattgt gctgggaatg ttgctggctt
     7861 ccaccatcat cttatgtaac tacaccaaca acaccacgct ggtggtcatg ctgatggcgc
     7921 tggctttttt tggcaaagga tttggtgctc tgggctggcc ggtgatttct gacagcgcgc
     7981 cgaaagagat tgttggcctc tgtggcggcg tctttaacgt ctttggcaat gttgcctcca
     8041 ttgtcactcc actggtgatt ggctacctgg taagtgaact gcactccttc aatgcagcac
     8101 tggttttcgt gggatgttca gcgctgatgg cgatggtctg ctacctcttc gtagttggcg
     8161 acattaaacg tatggaattg cagaaataag caaaggtata agcgatgaat aacgatgttt
     8221 tcccgaataa attcaaagcc gcactggctg cgaaacaggt acaaattggt tgctggtcag
     8281 cactctctaa cccgattagc actgaagttc ttggtttggc tgggtttgac tggctggtgc
     8341 tggatggcga acatgcgcca aacgatatct ccacgtttat tccgcagtta atggccttga
     8401 aaggcagcgc cagcgcgcca gtagtgcgag tgccgaccaa cgagccggta attattaagc
     8461 gtcttctgga tatcggtttc tataacttcc tgattccttt tgtagaaaca aaagaggaag
     8521 cagagcaggc ggtggcatca acccgttacc caccggaagg cattcgcggc gtctccgttt
     8581 ctcaccgcgc caatatgttt ggcaccgtgg cggattattt cgctcagtcg aacaagaaca
     8641 tcactattct ggtccagata gaaagtcagc agggcgtaga taacgtcgat gccattgccg
     8701 ctaccgaagg cgtagacggc atcttcgtcg gccccagcga tctggccgcg gcattaggcc
     8761 atctcggcaa tgcatcacac ccggatgtac aaaaagcaat tcagcacatt tttaaccgtg
     8821 ccagcgcgca cggcaaaccc agcggtatcc tcgcgccggt cgaagccgat gcgcgtcgtt
     8881 atctggaatg gggcgcgacg tttgtggctg tcggcagcga tctcggcgtc ttccgctctg
     8941 ccactcataa actggctgat acctttaaaa aataaccacc accgcaacaa gagagatgat
     9001 tgatatgact atgaaagttg gttttattgg cctggggatt atgggtaaac caatgagtaa
     9061 aaaccttctg aaagcaggtt actcgctggt ggttgctgac cgtaacccag aagctattgc
     9121 tgacgtgatt gctgcaggtg cagaaacagc ctctacggct aaagcgatcg ctgaacagtg
     9181 cgacgtcatc ataaccatgc tgccaaactc ccctcatgtg aaagaggtgg cgctgggtga
     9241 gaatggcatt attgaaggcg cgaagccagg tacggtattg atcgatatga gttctatcgc
     9301 accgctggca agccgtgaaa tcagcgaagc gctgaaagcg aaaggcattg atatgctgga
     9361 tgctccggtg agcggcggtg aaccgaaagc catcgacggt acgctgtcag tgatggtggg
     9421 cggcgacaag gctattttcg acaaatacta tgatttgatg aaagcaatgg cgggttccgt
     9481 ggtgcatacc ggggcaattg gtgcaggtaa cgtcaccaaa ctggcgaatc aggtcattgt
     9541 ggcgctgaat attgccgcga tgtcagaagc gttaacgctg gcaactaaag cgggcgttaa
     9601 cccggacctg gtgtatcagg caattcgcgg tggactggcg ggcagtaccg tgctggatgc
     9661 caaagcgccg atggtgatgg accgcaactt caagccgggc ttccgtattg atctgcatat
     9721 taaggatctg gcaaatgcgc tggatacttc ccacggcgtc ggcgcgcaac tgccgctcac
     9781 agctgcggtt atggagatga tgcaggcact gcgagcagat ggtttaggaa cggcggatca
     9841 tagcgccctg gcgtgctact acgaaaaact ggcgaaagtc gaagttactc gttaatgacg
     9901 tcgtgcccgg atggcgtatt gcaatccggg cctggaatcc aggccgaata agagaaacgc
     9961 cctccggcgt catgtggtaa caggcatagg tatgaaaatc gtaatcgccc cagactctta
    10021 taaagaaagt ttatctgcca gcgaggttgc gcaggcgata gaaaaaggat ttcgggaaat
    10081 ttttcctgat gcacagtacg tttctattcc ggttgccgac ggtggcgaag gaacggtgga
    10141 agcgatgatt gcagccaccc agggttctga acgtcacgcc tgggttacag ggccgctggg
    10201 cgagaaagtg aatgccagtt gggggatctc cggcgatggc aaaaccgcgt ttattgaaat
    10261 ggcggcggcc agtgggctgg agctggtacc tgcggaaaaa cgcgatccac tcgtgaccac
    10321 ttcacgcggc acaggcgagt taatcctgca ggcgctggag agcggcgcga caaacattat
    10381 tatcggcatt ggcggtagcg ctacaaatga tggcggcgca ggcatggtac aggcgctggg
    10441 ggcgaaatta tgcgacgcca acggcaatga aattggtttt ggcggcggta gtcttaatac
    10501 tctgaatgat attgatattt ccggcctcga tccgcgctta aaagattgcg tcattcgcgt
    10561 cgcttgtgat gtcaccaatc cgctggtggg cgataacggc gcatcgcgca tctttggccc
    10621 acaaaaggga gccagtgaag cgatgattgt tgagctggac aataacctct ctcactatgc
    10681 cgatgtcatt aaaaaagcgc tgcatgttga tgtaaaagat gtccccggcg caggagctgc
    10741 gggcggtatg ggtgcggcgc tgatggcgtt tcttggtgcg gaactgaaaa gtggtattga
    10801 aatcgtcact acggcgctga atctggagga acatattcac gattgtacgc tggtgatcac
    10861 cggtgaaggg cgtattgaca gccagagtat tcacggcaag gtaccgattg gcgtcgcaaa
    10921 cgtggcgaag aagtaccata aaccggtgat tggcattgcg ggtgcgggta gcctgaccga
    10981 tgatgttggc gttgtacatc agcatggcat tgatgcggtc ttcagcgtat tgaccagcat
    11041 aggtacgttg gacgaagcat tccgcggggc ttatgacaat atctgccgtg cttcacgtaa
    11101 tattgccgcg acactggcga ttggaatgcg caacacgggg tgacaagggc gcgcaaaccc
    11161 tctatactgc gcgccgaagc tgaccagaca gtcgccgctt cgtcgtcgtc ctcttcgggg
    11221 gagacgggcg gaggggagga aagtccgggc tccatagggc agggtgccag gtaacgcctg
    11281 ggggggaaac ccacgaccag tgcaacagag agcaaaccgc cgatggcccg tgcaagcggg
    11341 atcaggtaag ggtgaaaggg tgcggtaaga gcgcaccgcg cggctggtaa cagtccgtgg
    11401 cacggtaaac tccacccgga gcaaggccaa ataggggttc ataaggtacg gcccgtactg
    11461 aacccgggta ggctgcttga gccagtgagc gattgctggc ctagatgaat gactgtccac
    11521 gacagaaccc ggcttatcgg tcagtttcac ctgttttacg taaaaacccg cttcggcggg
    11581 tttttgcttt tggaggggca gaaagatgaa tgactgtcca cgacgctata cccaaaagaa
    11641 agcggcttat tggtcagttt cacctgatgt acgtaaaaac ccgcttcggc gggtttttgc
    11701 ttttggaggg gcagaaagat gaatgactgt ccacgacgct atacccaaaa gaaagcggct
    11761 tatcggtcag tttcacctga tatacgtaaa aacccgcttc ggcgggtttt tgcttttgga
    11821 ggggcagaaa gatgaatgac tgtccacgac actataccca aaagaaagcg gcttatcggt
    11881 cagtttcacc tgatgtacgt aataaaccgt tccggcgggt ttcagattgt tgagtgcgct
    11941 ttattcatgc cggatgctgc gtgaacgcct tatcctgcct acaaaagcac gcaaattcaa
    12001 tatattgcag agatggcgta ggcctgataa gcgtagcgca tcaggcattt ttgcggttgc
    12061 catcagtttc aaacccgcgt cgataagttt ttgcttttgg tgcacagggt aagatgaata
    12121 tctacggtat taataataga ctttgtaaga agttattaag tctatattgc atttaaaagg
    12181 tcgtaattat acgtatagtc ctctctggta aaatattgat tctctgagtt atatgcttca
    12241 tatgtgttat attccccgac ctcaaagcca ttgtccgtag aattttcgtc tttctcgagg
    12301 gtattaactc ttctgagcta gagtcttcta tgtttaaatc catagtataa ttaatttggg
    12361 ggacgaccag ataagcattt tctttgatat tttcataatc ttcaatgatt gtatcgatgt
    12421 aatcatatat tttttttgat gagaattctt tcatctctgg taatttaatt ctgttcaagc
    12481 aattgttgat tttcgatgag cttttatata tttcttttgt taattcattt tctaatttat
    12541 ctgtaagagt tgcccttggt atagatttta taattgcaaa tctcataatt tttaattgtt
    12601 cttcggtaat gtctccaagc tcatttaaac aactttcgac aaacttgatt ttttcttcag
    12661 aatacatatc gctaatgctt attaaaacag gcgttatttt tagtaaataa tcccaggaga
    12721 ttgtggcgtt ttgtgttgaa ctaacaaaat taacctgaaa catgttggca ttagtaaaat
    12781 ttgttgaatc caatttaaaa tgatttagaa tagtattagc catatgttta tctgtgaaat
    12841 cataataatc taaatcgaca ttacgtaagt ccagagagga tagatcaact atatctccag
    12901 tcccacaact aaagattacg ttcaacctcc tgatttcatt gtaaatatct tttaatgggc
    12961 aattaacatt gatattgcct gtagatattt cctggttttt taacggttca ttaatggtaa
    13021 gttcgcaggt attgtccgat cgttgaataa tttgggcgtt ataacctaaa aaatccagac
    13081 gaagagacga gtcattataa agaattacat tttgtaagta cgctaattct ttttgaaaat
    13141 caccagtgat aatgcatttt ccacttggtt gtaacgcgtt aagtaaagtg accatttgaa
    13201 tagaggtctg ttttagtttt tctataccgg gtgctagtgc ttcgaggtta tttcctatgg
    13261 ggctttcagg aaggtcattc atttttcgac ggactaaatc actactaata tcattaccaa
    13321 ttgatgaaac agggaacatt attttatcct ttataaataa tcaataaaaa tcattattaa
    13381 aatgatcggg tgaaaaaaag agaatttgcc aacaatctga agtaatgcag gttttattaa
    13441 agtgcctagg gggtgttaag atggcttctt tttgggtgaa ctttgcgcaa tacttagaaa
    13501 tcgtctcctt aatacattcc agctcttttt tattgacggc tgagcaagta cattgttcac
    13561 caaaaggtgc aaaagtgcct acatctaatg tttgtatgat ataatcatca tgttctcctg
    13621 tagaaatatc tatgatgaat ggactcatat tttccggttg gattcggatc atgacagaag
    13681 gaacatcgcc ttgatagaaa ttcaatgcag taattgcaga taatactctc cgcagttcat
    13741 tacttatact gtaaatttta ttaacgtcat tggtggagac aatttctgca aactttcttt
    13801 ttaccgtcca ataatcaata tttctattgt gattgttaac cactgcgtgc attggcggga
    13861 ttgaagggtg aaaaatatgt gcaattggga agcctttcat tgatgcctgc cttgtcgtca
    13921 tgacaaacaa taatagatgc ttattttcct cgcacgacac ttacgcacta tcaaaaaacg
    13981 ctcatttacc cggcgttaca gtaaacgaag caacattctg tgtcctacgc tgatgtaaag
    14041 cacagtgaaa atcccccttg ttgacttcgt ctgaccttat cgcggcgacc agcaattatt
    14101 cgttttgtga tcctcaacac aaaatgctat tcgtcgccta tgaggggaga agaatttatg
    14161 gaaaacgatt gcattcacaa aatgacgtcc tgtcagaaga gaaaaagttg acgttaatca
    14221 attgttcgag atgatcaata cctgtattcg aaattttaaa atattgataa aataaatgtt
    14281 tgttctccaa caaatagcgt gaaaaaatgt gacgtgccgc agtatttatc gctgaaaatg
    14341 gtttatttat aataaaattt gagaacttgc tcattaaatt taactcaaat tttgcctggt
    14401 aattatccgg taattgcttg aaatatggtc tcagcccctt ttttgtatta accacataac
    14461 gaatgatgtt atcgccataa aatatggtta tccccgtcat tttttttgat agaaatcagg
    14521 gtttatgctg atttttatgc tttaacttgt tgatatttaa aggtatttaa ttgtaataac
    14581 gataccttgg aaagtaatga aagttaattt gtgagtggtc gcacatatcc tgtttatttc
    14641 attttgatac acttcatgcc gtcaatgagg taattaacgt aggtcgttat gagcactatt
    14701 cttcttccga aaacgcagca cctggtagtc tttcaggaag tcattagaag tggctctatc
    14761 ggctcggctg caaaagaatt agggttaact caaccggccg tcagtaaaat cattaacgat
    14821 attgaagatt attttggtgt ggaattagtg gtgcggaaaa ataccggtgt aacattaaca
    14881 cctgccggtc aattgttact ctcccgttcc gaatccatta cccgtgaaat gaaaaatatg
    14941 gttaatgaga taagcggtat gtcttctgag gcggtggtgg aagtctcatt tggttttcct
    15001 tcattgattg gttttacttt tatgtcaggg atgatcaaca agttcaaaga ggtgttcccg
    15061 aaagcgcagg tttctatgta tgaagcgcaa ctgtcttcgt tcttaccggc aatccgcgac
    15121 ggtcgactgg attttgcgat tggtacgtta agtgcagaaa tgaagcttca ggatttacat
    15181 gttgagccgc tgttcgagtc cgagtttgtg ctggtagcca gtaagtcccg aacatgcacc
    15241 ggcaccacca cgctggagtc gttgaagaac gaacagtggg tgttgccaca aactaatatg
    15301 gggtactaca gcgaactgct tactacgtta caaagaaatg gcatcagtat tgaaaacatc
    15361 gttaaaaccg actcagtcgt gacaatttat aatcttgttc tcaatgctga tttcttaact
    15421 gtaattcctt gtgatatgac gtcacctttt ggttctaatc aatttattac tattccggtt
    15481 gaagaaacat tacctgtggc acaatatgcc gcggtatggt cgaaaaatta tcgtattaaa
    15541 aaagcagcat cggttttggt ggaattagcc aaagagtatt catcttataa tgggtgtaga
    15601 cgaaggcaat taatagaagt tggttagtta tttgttttta tttaaacata aataatccac
    15661 ctgtctgttt tgcaggtgtc ggttacggtt acctacatat ttaattcagg cgaagaggtt
    15721 ttataatgca tattacatac gatctgccgg ttgctattga tgatattatt gaagcgaaac
    15781 aacgactggc tgggcgaatt tataaaacag gcatgcctcg ctccaactat tttagtgaac
    15841 gttgcaaagg tgaaatattc ctgaagtttg aaaatatgca gcgtacgggt tcatttaaaa
    15901 ttcgtggcgc atttaataaa ttaagttcac tgaccgatgc ggaaaaacgc aaaggcgtgg
    15961 tggcctgttc tgcgggcaac catgcgcaag gggtttcgct ctcctgcgcg atgctgggta
    16021 tcgacggtaa agtggtgatg ccaaaaggtg cgccaaaatc caaagtagcg gcaacgtgcg
    16081 actactccgc agaagtcgtt ctgcatggtg ataacttcaa cgacactatc gctaaagtga
    16141 gcgaaattgt cgaaatggaa ggtcgtattt ttatcccacc ttacgatgat ccgaaagtga
    16201 ttgctggcca gggaacgatt ggtctggaaa ttatggaaga tctctatgat gtcgataacg
    16261 tgattgtgcc aattggtggt ggtggtttaa ttgctggtat tgcggtggca attaaatcta
    16321 ttaacccgac cattcgtgtt attggcgtac agtcagaaaa cgttcacggc atggcggctt
    16381 ctttccactc cggagaaata accacgcacc gaactaccgg caccctggcg gatggttgtg
    16441 atgtctcccg cccgggtaat ttaacttacg aaatcgttcg tgaattagtc gatgacatcg
    16501 tgctggtcag cgaagacgaa atcagaaaca gtatgattgc cttaattcag cgcaataaag
    16561 tcgtcaccga aggcgcaggc gctctggcat gtgctgcatt attaagcggt aaattagacc
    16621 aatatattca aaacagaaaa accgtcagta ttatttccgg cggcaatatc gatctttctc
    16681 gcgtctctca aatcaccggt tttgttgacg cttaattaat tcgttgagga taggatatga
    16741 gtacttcaga tagcattgta tccagccaga cgaaacaatc gtcctggcgt aaatcagata
    16801 ccacatggac gttaggcttg tttggtacgg caatcggcgc cggggtgctg ttcttcccta
    16861 tccgcgcagg ttttggcgga ctgatcccga ttcttctgat gttggtattg gcatacccca
    16921 tcgcatttta ttgccaccgg gcgctggcgc gtctgtgtct ttctggctct aacccttccg
    16981 gcaacattac ggaaacggtg gaagagcatt ttggtaaaac tggcggcgtg gttatcacgt
    17041 tcctgtactt cttcgcgatt tgcccactgc tgtggattta tggcgttact attaccaata
    17101 cctttatgac gttctgggaa aaccagctcg gatttgcacc gctgaatcgc ggctttgtgg
    17161 cgctgttcct gttgctgctg atggctttcg tcatctggtt tggtaaggat ctgatggtta
    17221 aagtgatgag ctacctggta tggccgttta tcgccagcct ggtgctgatt tctctgtcgc
    17281 tgatccctta ctggaactct gcagttatcg accaggttga cctcggttcg ctgtcgttaa
    17341 ccggtcatga cggtatcctg atcactgtct ggctggggat ttccatcatg gttttctcct
    17401 ttaacttctc gccaatcgtc tcttccttcg tggtttctaa acgtgaagag tatgagaaag
    17461 acttcggtcg cgacttcacc gaacgtaaat gttcccaaat catttctcgt gccagcatgc
    17521 tgatggttgc agtggtgatg ttctttgcct ttagctgcct gtttactctg tctccggcca
    17581 acatggcgga agccaaagtg cagaatattc cagtgctttc ttatctggct aaccactttg
    17641 cgtccatgac cggtacgaaa acaacgttcg cgattacact ggaatatgcg gcttccatca
    17701 tcgcactcgt ggctatcttc aaatctttct tcggtcacta tctggggacg ctggaaggct
    17761 tgaatggtct gattctgaag ttcggttata aaggtgacaa aaccaaagtg tcgctgggta
    17821 aactgaatac tctcagcatg atcttcatca tgggctccac ctgggttgtt gcctacgcca
    17881 acccgaacat cctcgacctg attgaagcca tgggcgcacc gattatcgca tccctgctgt
    17941 gcctgttgcc gatgtatgct atccgtaaag cgccgtctct ggcgaaatac cgtggtcgtc
    18001 tggataacgt gtttgttacc gtgattggtc tgctgaccat cctgaacatc gtatacaaac
    18061 tgttttaatc cgtaactcag gatgagaaaa gagatgaatg aatttccggt tgttttggtt
    18121 attaactgtg gttcgtcttc gattaagttt tccgtactcg atgccagcga ctgtgaagta
    18181 ttaatgtcag gtattgccga cggtattaac tcggaaaatg cattcttatc cgtaaatggg
    18241 ggagagccag caccgctggc tctccacagc tacgaaggtg cattgaaggc aattgcattt
    18301 gaactggaaa aacggaattt aaatgacagc gtggccttaa ttggccaccg catcgctcac
    18361 ggcggcagta tttttaccga gtccgccatt attaccgatg aagtcattga taatatccgt
    18421 cgcgtttctc cactggcacc cctgcataat tacgcaaatt taagtggtat tgaatcggcg
    18481 cagcaattat ttccgggcgt aactcaggtg gccgtatttg ataccagttt ccaccagacg
    18541 atggctccgg aagcttattt atacggcctg ccgtggaaat attatgaaga gttaggtgta
    18601 cgccgttatg gtttccacgg cacgtcgcac cgctatgttt cccagcgcgc acattcgctg
    18661 ctgaatctgg cggaagatga ctccggcctg gttgtggcgc atcttggcaa tggcgcgtca
    18721 atctgcgcag ttcgcaacgg tcagagtgtt gacacctcaa tgggaatgac gccgctggaa
    18781 ggcttgatga tgggtacccg cagtggcgat gtcgactttg gtgcgatgtc ctggatcgcc
    18841 agccaaacca accagagcct gggtgacctg gaacgcgtag tgaataaaga gtcgggatta
    18901 ttaggtattt ccggtctttc ttcggattta cgtgttctgg aaaaagcctg gcatgaaggt
    18961 cacgaacgcg cgcaactggc aattaaaacc tttgttcacc gaattgcccg tcatattgcc
    19021 ggacatgcag cttcattacg tcggctggat ggaattatat tcaccggcgg aataggagag
    19081 aattcaagtt taattcgtcg tctggtcatg gaacatttgg ctgtattagg cgtagagatt
    19141 gatacagaaa tgaataatcg atctaactct tttggcgagc gaattgtttc cagtgaaaat
    19201 gcgcatgtca tttgcgtcgt tattccgact aacgaagaaa aaatgattgc tttggatgcc
    19261 attcatttag gtaaagttaa cgcgcccgca gaatttgcat aatttagttg aagtattgta
    19321 gagagattat ttttcatgaa ggtagatatt gataccagcg ataagctgta cgctgacgca
    19381 tggcttggct ttaaaggtac ggactggaaa agcgaaatta atgtccgcga ttttattcaa
    19441 cataactata caccgtatga aggcgatgaa tcttttctcg ccgaagcgac gcctgccacc
    19501 acggaattgt gggaaaaagt aatggaaggc atccgtatcg aaaacgcaac ccacgcgccg
    19561 gttgatttcg ataccaatat tgccaccaca attaccgctc atgatgcggg atatattaac
    19621 cagccgctgg aaaaaattgt tggcctgcaa acggatgcgc cgttgaaacg tgcgctacac
    19681 ccgttcggtg gcattaatat gattaaaagt tcattccacg cctatggccg tgaaatggac
    19741 agtgaatttg aatatctgtt taccgatctg cgtaaaaccc ataaccaggg cgtatttgat
    19801 gtttactcac cggatatgct gcgctgccgt aaatctggcg tgctgaccgg tttaccagat
    19861 ggctatggtc gtgggcgcat tatcggtgac tatcgccgcg tagcgctgta tggcatcagt
    19921 tatctggtgc gtgaacgcga actgcaattt gccgatctcc agtctcgtct ggaaaaaggc
    19981 gaggatctgg aagccaccat ccgtctgcgt gaggagctgg cagagcatcg tcatgcgctg
    20041 ttgcagattc aggaaatggc ggcgaaatat ggctttgata tctctcgccc ggcgcagaat
    20101 gcacaggaag cggtgcagtg gctctacttc gcttatctgg cggcagtgaa atcgcaaaat
    20161 ggcggcgcga tgtcgctggg gcgcacggca tcgttcctgg atatctacat tgagcgcgac
    20221 tttaaagctg gcgtcctcaa tgagcagcag gcacaggaac tgatcgatca cttcatcatg
    20281 aagatccgta tggtacgctt cctgcgtaca ccggaatttg attcgctgtt ctccggcgac
    20341 ccaatctggg cgacggaagt gatcggcggg atggggctgg acggtcgtac gctggtgacc
    20401 aaaaactcct tccgctattt gcacacctgc acactatggg gccggcaccg gaacctaacc
    20461 tgaccattct ttggtcggaa gaattaccga ttgccttcaa aaaatatgcc gcgcaggtgt
    20521 cgatcgtcac ctcttccttg cagtatgaaa atgacgatct gatgcgtact gacttcaaca
    20581 gcgacgatta cgcgattgcc tgctgcgtca gcccaatggt gattggtaag caaatgcagt
    20641 tctttggtgc acgcgctaac ctggcgaaaa cgctgctcta cgcaattaac ggcggggtgg
    20701 acgagaagct gaagattcag gtcgggccga aaacagcacc gctgatggac gacgtgctgg
    20761 attacgacaa agtgatggac agcctcgatc acttcatgga ctggctggcg gtgcagtaca
    20821 tcagcgcgct gaatatcatt cactacatgc acgacaagta cagctacgaa gcttcgctga
    20881 tggcgctgca cgatcgtgat gtctatcgca ctatggcatg cggcatcgcg ggcctgtcgg
    20941 tggcgacgga ctccctgtct gccatcaaat atgcccgcgt gaaaccaatc cgtgacgaaa
    21001 acggcctggc ggtggacttt gaaatcgacg gtgaatatcc gcagtacggc aacaacgacg
    21061 agcgcgtaga cagcattgcc tgcgacctgg ttgaacgctt tatgaagaaa attaaagcgc
    21121 tgccaaccta tcgcaacgcc gtccctaccc agtcgattct gactatcact tctaacgtgg
    21181 tgtacggcca gaaaaccggt aatacgccgg acggtcgtcg cgccggaaca ccgttcgcgc
    21241 cgggcgctaa cccgatgcat ggtcgtgacc gcaaaggtgc cgtggcctca ttgacgtcgg
    21301 tggcgaaact gccgttcacc tacgccaaag atgggatctc gtacaccttc tcaatcgttc
    21361 ctgcggcgct gggcaaagaa gatccagtac gtaaaaccaa ccttgtcggc ctgctggatg
    21421 ggtatttcca ccacgaagcg gatgtcgaag gcggtcaaca cctcaacgtc aacgtaatga
    21481 atcgggaaat gctgctggat gccatcgagc acccggaaaa atatcctaac ctgacaatcc
    21541 gtgtctctgg ctacgccgtg cgcttcaacg cactgacccg tgaacagcaa caggatgtta
    21601 tttcacgtac ttttacccag gcgctctgac gccggaggat gtatgaaaaa gattatcgaa
    21661 acgcaacgtg ccctaggcgc aatcggccct tatgttcagg gcgttgattt aggcagcatg
    21721 gtcttcacct ccgggcaaat tccggtttgc ccgcagaccg gtgagatccc ggctgatgtg
    21781 caagatcagg cgcgtttaag cctcgaaaac gtcaaagcga tcgtggttgc tgccgggctg
    21841 agcgtgggcg atatcatcaa gatgaccgtg ttcatcaccg atctgaatga ttttgccacc
    21901 atcaatgaag tctataagca gttcttcgat gagcatcagg cgacctaccc gacccggagc
    21961 tgtgtgcagg ttgcgcgttt gccgaaagat gtgaagctgg aaattgaagc tatcgcagta
    22021 cgtagtgcgt aaagcctcgt gagcgggacg gtcgcaaggt cgttccgctc cacttcactg
    22081 aacggcaatc cgagggtgtg gatatgatta gtgcattcga tattttcaaa attgggattg
    22141 gtccctccag ttcgcatacc gtggggccaa tgaatgccgg aaaaagtttt attgatcggc
    22201 tggaaagtag cggcttatta accgcgacga gccatattgt ggtcgatctg tacgggtcgt
    22261 tgtcactgac gggcaaaggc catgccacgg atgtcgccat tatcatggga ctggcaggaa
    22321 acagtccgca ggatgttgtc attgatgaga tccctgcatt tatagagtta gtaacgcgca
    22381 gcgggcggct gccagtggca tctggtgcgc atattgttga ttttcctgta gcaaagaaca
    22441 ttatcttcca tcccgaaatg ttgcctcgcc atgagaacgg aatgcggatc actgcctgga
    22501 agggacagga agcgctatta agtaaaacct attactctgt cggcggcggg tttattgtcg
    22561 aagaagaaca cttcggcctg tcgcacgatg tcgaaacgtc cgtaccttac gatttccact
    22621 cagcaggtga actgctgaaa atgtgtgatt acaacggcct gtctatatct ggcctgatga
    22681 tgcacaacga gctggcgctg cgcagcaaag cggaaattga cgcagggttt gcccgtatct
    22741 ggcaagtgat gcatgacggt attgaacgcg ggatgaacac tgaaggcgtg ctgcctggtc
    22801 cgctcaatgt gccgcgtcgt gccgtagcgc tgcgtcgtca gctggtttct agcgataaca
    22861 tctctaacga tccgatgaat gtcatcgact ggatcaacat gtacgcgctg gcggttagtg
    22921 aagaaaacgc agctggcggg cgcgtggtaa cggcaccgac taacggtgcg tgcggcatta
    22981 ttccggcagt gctggcttat tacgataagt tccgtcgtcc ggtaaacgag cggtcaattg
    23041 cccgctattt tctggcagcg ggggctattg gcgcgctgta taaaatgaac gcctccatct
    23101 ctggcgcgga ggtcggctgt cagggggaga ttggcgtggc ctgttcaatg gcagcggcag
    23161 ggttaactga actactgggc ggcagtccgg cgcaggtatg caatgcggcg gaaatcgcga
    23221 tggagcataa ccttgggctg acctgcgatc cggttgccgg acaggtacaa atcccgtgca
    23281 ttgaacgtaa tgccattaat gccgtgaaag cagtaaacgc cgcgcggatg gcgatgcgcc
    23341 gcacctcggc accgcgtgtt tcactcgata aagtgatcga gacgatgtat gaaaccggca
    23401 aagatatgaa cgataaatac cgcgaaacat cacgcggagg actggccatt aaagtggtct
    23461 gcgactgaca ttgctgtcag ctttcatcct tgggtgcaca aatgtgcacc ttttttttgt
    23521 gatctgccgc aaatagagaa tgaatattct cttgcgatta atttagtaga aaattcttat
    23581 ccatctccgt tattttaaag tcttgattgt gaagattttc tctgacctcg cgcgtatcat
    23641 ttctttattc taataactcg cctgcaaaaa cgtagaccgc atatcgcctt gatgcggctc
    23701 ggaaaaaaat aataagccct acaaaatatt gagcgagaaa ttatggaaat tgcatcgaat
    23761 aaaggcgtca ttgcagacgc ttcgaccccg gcgggtcgtg ctggaatgag tgagagcgag
    23821 tggcgagaag cgatcaaatt cgacagtact gacaccggct gggtgattat gagtatcggg
    23881 atggcgattg gcgcggggat tgtttttctc ccggtgcagg tcggtttgat gggattgtgg
    23941 gtatttttgc tctcatcggt gattggttac ccggcaatgt atctgtttca gcggttgttt
    24001 attaatacgc tggcagaatc accagaatgt aaagattacc cgagtgtcat tagcggttat
    24061 ttaggtaaaa actggggcat cctgttaggt gcgctctatt tcgtaatgct ggtgatttgg
    24121 atgttcgtct attccaccgc catcaccaac gatagtgctt cctatctgca taccttcggc
    24181 gtgacggaag ggttgctgtc agacagtccc ttttatggtc tggtactgat ttgcattctg
    24241 gtggcgatct cttcacgcgg cgagaaattg ttattcaaaa tttcgaccga catggtgctg
    24301 accaagctgc tggtagtcgc tgcgctgggc gtgtcgatgg tgggaatgtg gcatctgtat
    24361 aacgtcggtt cgctaccgcc gctggggctg ctggtgaaaa acgccattat tacgctgccg
    24421 tttaccctga cgtcgattct gtttatccag acgttaagcc cgatggtgat ctcttatcgc
    24481 tcgcgggaaa aatcaattga agtggcgcgg cataaagcat tgcgggcaat gaatatcgcg
    24541 tttggcattt tgtttgtcac cgtctttttc tactccgtgt cgttcacgct ggcgattgcc
    24601 gcacagttta ttagcggtga cggcgcagcg tgggtgaaag tggtcagcgt cattctcaat
    24661 atctttgctg tcatgactgc attctttggc gtctatttag gctttcgcga agcaacgcaa
    24721 gggatcgtaa tgaacatcct gcgtcgcaag atgcctgccg agaagattaa cgaaaatctt
    24781 gttcagcgcg gcatcatgat tttcgccatt ttgctggcct ggagcgccat cgtactgaac
    24841 gcaccggtgt tgagcttcac ctctatctgt agcccgattt tcggcatggt agggtgcctg
    24901 atcccggcgt ggctggttta caaagtaccg gcattgcaca aatacaaagg gatgtctctg
    24961 tacctgatta tcgtcactgg tttgttgctt tgtgtttctc tgttcctggc attttcttga
    25021 tttacctgaa attttaaggt ttttaatatg tttgattcga ctttaaatcc gttatggcag
    25081 cgttacatcc tcgccgttca ggaggaagta aaaccggcgc tgggatgtac tgaaccgatt
    25141 tcactggcgc tggcggcggc ggttgctgcg gcagaactgg aaggtccggt tgaacgtgta
    25201 gaagcctggg tttcgccaaa tctgatgaag aacggtctgg gcatcaccgt tcccggcacg
    25261 ggaatggtgg ggctgccgat tgcggcggcg ctgggggcgt taggtggaaa tgccaacgcc
    25321 gggctggaag tgctgaaaga cgcaactgcg caggcaattg ccgatgccaa agcactgctg
    25381 gcggcgggga aagtctctgt taagatccag gaaccttgca atgaaatcct cttctcacgc
    25441 gccaaagtct ggaacggtga gaagtgggcg tgtgtcacca tcgtcggcgg gcataccaac
    25501 attgtgcata ttgagacgca caatggtgtg gtgtttaccc agcaggcgtg tgtggcagag
    25561 ggcgagcaag agtcgccgct gacggtgctt tccagaacga cgctggctga gatcctgaag
    25621 ttcgtcaatg aagtcccatt tgcggcgatc cgctttattc tcgattccgc gaagctaaat
    25681 tgtgcgttat cgcaggaagg tttgagcggt aagtgggggc tgcatattgg cgcgacgctg
    25741 gaaaaacagt gcgagcgcgg tttgctggcg aaagatctct cttcatccat tgtgattcgt
    25801 accagcgcgg catccgatgc gcgtatgggc ggcgctacgc ttccggctat gagtaactcc
    25861 ggctcgggta accaggggat caccgcaaca atgcctgtgg tggttgtagc agaacacttc
    25921 ggagcggatg atgaacgact ggcgcgtgcg ctgatgcttt ctcatttgag cgcaatttac
    25981 atccataacc agttaccgcg tttgtctgca ctttgtgccg caacgaccgc agcaatgggg
    26041 gccgccgccg ggatggcatg gctggtggat gggcgttatg aaactatctc gatggcgatc
    26101 agcagtatga tcggcgatgt cagcggcatg atttgcgatg gtgcgaaggt ttcgaccagt
    26161 gcttcggctg cgtggaaagc ggtgttaatg gcgctggatg ataccgccgt gaccggcaat
    26221 gaagggatcg tggcgcatga tgttgagcag tcgattgcca acctgtgtgc gttagcaagc
    26281 cattcgatgc agcaaacgga tcggcagatt atcgagatta tggcgagcaa ggccagataa
    26341 acgccaggtt ttgagtattg tgccggatgc gatgctggcg caccttatcc ggcctacggg
    26401 gtgctcaact tgttgatgtt gttgtgtttt gtacctgatg ccggatgcga tgctggcgca
    26461 tcttatccgg cctacggggt gtttaatttg ttgatgttgt tgtgttttgt aggccggata
    26521 aggcgtttac gccgcatccg gcaacctgtc tttacgcggt agcttcatcc tcggctaaac
    26581 gcgtttcagc atccgcgacg atagcttcca gctcgctcaa catctcttca tagccaaaat
    26641 ttttggcggt gcgagcaggt tctttcgcca ccacgggctt cgctagctgg cgctttttgg
    26701 tcgatttttt actcatcatc atctccctgt aaaaacaact agacaggcaa atctatcagc
    26761 aaagcgcgca gtggggaatc ggcaaccagc gtaatgttag cctcgtcacg aataaacgcc
    26821 ccatcaccgc aggttagcgc cgctttctct tcatgatgcg taagcgcatg aaatttcccg
    26881 tgaatcgatt gcaaatacgc gcgtggccca tgcaactgga aattcgcact ttcgcctttg
    26941 tcgagcacga tatggtgcag ccacacttgt tggcgtaatt gcaggcttcc catcgcaccc
    27001 tctggcgagg cgattaattg ctgcttgtcc atattaagcg ccagcttttg aatcagcgga
    27061 ttctctcgct gcgggcaggt atccagccaa agctgcattc gcgttaacgg tttgtctttg
    27121 ctgagattgt gttcgctata gctgacaccc ggctgggtag agagcagcaa cgcctctccg
    27181 gcgctggcct gaacatgatt gccttcgctg tcgcgatact ctgcttctcc atccagaatc
    27241 acatttaaaa tatcgacttt gggataggtt cgcggctgaa aggcggcacc tggggccagc
    27301 acttcctggt taagcacacg cagggaggca tagcctaaca atttcgggtc gaagtagtgt
    27361 ccaaaggaaa aagtataccg ggcctgcaac catccgtagt ctgcttgtcc acactgcctg
    27421 gcagttcggg tagtaatcat aattcttgac ctctctttac ttccttttat ggtaaggggc
    27481 tggacgctac attgttagcc agatattctg cccggtatgt tcaaatttcc tgaatgagaa
    27541 cgaaatggcc aaagaaaggg cattaacgct ggaagcacta cgggttatgg atgcgatcga
    27601 tcgccggggc agttttgcgg cggcagcgga tgagctggga cgcgtgcctt ccgcacttag
    27661 ctacatcatg caaaaactgg aagaagagct ggatgtggtg ctgtttgacc gctcgggcca
    27721 tcgcaccaaa ttcaccaatg tcgggaggat gttgctggag cgagggcgcg ttttgctgga
    27781 agccgcagat aaactgacta ctgatgcgga agctctcgcg cgcggttggg aaacgcatct
    27841 caccattgtg accgaagcgc tggtaccgac acctgccttt ttcccgttaa tcgacaaact
    27901 ggcggcaaaa gccaataccc aactggcaat catcacagaa gtgctggcgg gggcgtggga
    27961 acggctggag caggggcggg cggatatcgt tatcgcgccg gatatgcatt ttcgttcctc
    28021 gtcggagatc aactcgcgca agctctatac gttaatgaac gtctacgttg ccgcgcctga
    28081 tcacccgatt catcaggagc cggaaccgtt atctgaagtg acgcgtgtga aatatcgtgg
    28141 aattgcggta gcggataccg ctcgtgagcg cccggtgttg accgtacagc tgctggacaa
    28201 acagccgcgc ttaacggtga gcacgattga agataaacgt caggcattac tggcggggct
    28261 tggcgtggcg acgatgccgt atccgatggt cgaaaaagat attgcggaag ggcagttgcg
    28321 tgtcgtcagc ccggaatcga ccagcgagat cgatattatt atggcctggc gtcgtgacag
    28381 tatgggggaa gcgaaatcct ggtgtctgcg ggaaattcca aagttgttcg ctggaaaata
    28441 atctattaag cacctttcac aaggtgcttc tgactgctga caaacgcaaa actgcctgat
    28501 gcgctacgct tatcaggcct acgcagtcac tgcaatatat tgaattttgg caatttgtag
    28561 gctggataag gcgtttacgc cgcatccggc ataaacaaag cgcactttgt caacaatttg
    28621 aagcaccttt tacaaggtgc ttaatcatta ttcttgttcc agttttggat ctggtccaaa
    28681 gcggttttcg cctggcgtac ccgcctggca gttaaagacg atgataataa gccagccgat
    28741 aaacgggatt aagaatagca gtgcccacca cgccgagcgg tcggtgtcgt gcaggcggcg
    28801 gaactgaacc gcccaccacg gtaaaaacac cagaataccg tagattgtcg tcaggatgcc
    28861 ttcgccacca gcgcgttgcc agcctaacat tttatccagc agccccagca cgaacgtaaa
    28921 gatgatgttg accagaataa acatccagta ctctttgcgc cgcgcacgac cccggaaacc
    28981 gacataattt tttagtactt tcagatacca gtccattttt gcctcaattt tgctcaatca
    29041 cttgttttta aagcagcaaa ttaagaatag acgtgaatga aatggcagta aatattcgtt
    29101 caaggaatta ataaagtgcc gtgaaggcgc gctaagactg atggcaagcg caaaatcgcc
    29161 agatgcgcta cgcttatctg gcctacgcgg tcattgtaac atattgaata taaacgcttt
    29221 gtaggtttga taaggcgttc acgccgcatc cgacatgaac aacatgcact tagctaatta
    29281 tcagaggcac ctcgtaaggt gccttttttt aaccgaagcg aacatctcgt ccatgcggtt
    29341 catcgagatc ctgccacgga ccaattgaaa taatccccgt agggttgatg gtcttatggc
    29401 tgcggaagta atgattacgg atgtgatcga aattgacagt ttcggcgatc cccggcatct
    29461 ggtagatatc gcgcaggaag ccatacagat tcaggtaatc gctgatgcgg tgcttatcac
    29521 acttgaagtg ggtcacatac actggatcaa aacgcaccag cgtagtccac aggcgaatat
    29581 cggcttcggt tagctggttg ccggtcaggt aacgatgctg gcctaaaatc tgctccagtc
    29641 gcgccagcga ttcaaacact ttcgccaccg cctcgtcgta ggcttgctgg ctggtggcaa
    29701 aaccagcttt atacacgccg ttgttaacgg tgtcataaat ccagccgtta agttcgtcaa
    29761 ttttcgtttg cagggctggt gggtagtaat cacccgcttt tgcgcccagc gcatcaaacg
    29821 cggtattgaa catgcggatg atttctgctg attcgttgct gacgatggtg tggttctttt
    29881 tgtcccacag cacgggaaca gtaactcgtc cgctgtagtg tggatcggcg tggagataaa
    29941 gctgatacag aaattcatgt tgatagagcg tatctccggt tgctcccgga aaactgtcat
    30001 caaaggtcca gccgttttcc agcatcagcg ggttcactac ggaaacggaa ataaacggtt
    30061 ccagtccttt gagtttgcgc atgatcagcg tgcggtgcgc ccacgggcag gcgagtgaaa
    30121 catagagatg ataacgatct ttctctgcga taaaaccgcc tgtgccagtg gggccaggtg
    30181 cgccatcggc agtgagccag ttacgaaatg cggaagctga acgttgaaat ttaccgccgg
    30241 tagatttggt atcgtaccag gtgtcatgcc agacgccgtc aatcagttga cccatttttc
    30301 tctcctccaa tagcaaaaac gaggagcttc ctcctcgttt tttatcgtta agtatagcgc
    30361 gtgcttacca ctttttattc agcaggcggt cgatgctata cgcgcccgga ccggtaattg
    30421 ccagcagcag gaatccgcca gaaattgtca ggtttttcat gaacatcagc gagttgacgc
    30481 cttcagcaaa gttgctgtga aataaaaatg ccgtcagcag cgtaaagccc gcagtaaaca
    30541 gggttgtggt gcgagtcagg aaactgaata ggattgccag accaccaccg aactcaagca
    30601 gaatcaccag cggcagcata aaacccggga cacccattgc ttccatatat tgttgggtac
    30661 cagagtaagc ggtgattttt ccccagcctg cggtaataaa cagaatcggc attaaaatgt
    30721 gcgctaccag tacaccaaca tcttctaatt ttttcatcat actctccact gcgccactca
    30781 ggctgctgat tgtttttttg tgcaagggcg cggtattagc gtcgttgctg tcgatggaga
    30841 gaatcataaa cgtggtgaat gatgattgtt agcaaggaaa actgtcaaaa atattcaaaa
    30901 aatttgaggg ataaggccgg aattgctccg gccagaggga agttaaccgc gaagctgttg
    30961 ctgcttgagg gtcgttttaa ccagacgcca ggcgctccat acgccaaaac cgcgtctggc
    31021 ccagcggacc agcatattag gatggcgaat cgtccagatc gccatcacgc tactgccaac
    31081 cagcgcccag gagcgcagac ttagcagcat attccagcga cgatcgtaag cgcctgttgc
    31141 ctccagccat tcacgacgac tggcggaaag atccagccgt tgttgctgga tctggctaag
    31201 cagttgcgcc ttacgtcgtt cacgttcgac tttactgctc acgggactcc tcctcgagca
    31261 gttgccgatc gtttgccagc tcatggcgtg tatggcgcag caaggtggat ttacgcgatt
    31321 tacgtaacgt ccagataccg ccaatcagtg ccagtagcag caacaccacg gtggtggcaa
    31381 tcatcgcatt caggcgatat tgcggatcaa ccgcccaaat aattagcacc atcaggctca
    31441 taagaccaaa tgcagcgaaa agcatcgtca ggcccagcat cagtaaaagt tgaaagagat
    31501 tagctttttc ctcttccagc tccaccaccg ccagccgcag acgtgtttcc accatttcaa
    31561 ccatgatgga aacaattcgc tgcccgatgc ccagaacgct tttaccgggc ccttgcgcgt
    31621 gatgagtgtc cgccataatt aacgacgcga cagcagaacg ccgagcacta caccgattgc
    31681 agcgccaatt cccacgccgg tccacggatt ttcacgcaca tactcatcag cacgcgccgc
    31741 cgcgacacgg gtttgtttgg cgatggcatc accggtttca cccaggcgat aacggctctg
    31801 tttcagtgcc tgctccgctt tgctacgaat cttactcaac tcttctttcg acttctcgcc
    31861 agatgagcta agcacctctt ccagcgtatc ggaaagggat ttcaactcag cacgcagatg
    31921 ttccgtagtg tgttctttcg acatagtttt ctccaggtga gtaagtagtg actattgtga
    31981 gttagtagtc gcgcgcttcc agctttttca gctcttcctg cgcttctgcc agtttccgtt
    32041 ctatcggcat cgcctttttg cttcgcctcg gctaaatctt gctggcgttc cgccacctca
    32101 tctttctgct ttgcgatttt cttgtgatga tcggcacgca gctggctatc tgaacagttg
    32161 gcccggactt cactcagggc tttattcaga ccgtcaatac gattctggtt ttggtgtttt
    32221 tcggcatagc tgatctcctt aaggatattt tgctcctttt cctgacacag ggtagtggca
    32281 taactaccgg cactaagagc aaagagagaa acagctaaag cgatgcggta tttcattctt
    32341 gaaccttcca ttgtgattcg gacctattac aaaggaacga ttgtccagac aacgaagcgg
    32401 tagtgttaac caccgcctca ttaatcatag acagttacgg tgaaatcacc aaagtgagtg
    32461 atatgtttcg gataacagga agttatccga agcgatgaga gttatcccgt aaccgggtca
    32521 gccactgcat agcctgactg ttattgtcct gctgcgcaat gatgtagcca tggggcaatg
    32581 ttctgtcgag gaccgctttt gcggcggcgc tctggtcgct ggagtcaaat gtaattaaca
    32641 gagtgtcgtt tttgggggtg atacttttga aaggaatgcc atgagcgtca aggtgatgcc
    32701 agatcgaaaa accgtctggc atcgttgtgc cttgatgaac ggcacgaatc gccagcgtag
    32761 actcttgctg gcgaaccgcg gaccaggcca gcaacagcgt gccgaccaga agtaaaacag
    32821 tgccggacca ggctagctgg cgaagcgaca tgcgaggtat ttgcatccct tacccccgat
    32881 ttccatattt ctttttccat aacacgacca gagaacctgc caggccaaac accagcagca
    32941 ccaccgggag cagcatcagg catgacatca gctggtcctc gtactttaaa aataccggcg
    33001 ttttgccgag catgtaaccc agagttgtca ggatcaatac ccacagcaga ccgctcatcc
    33061 agttgaagaa ctgaaagcgc gcgttattca gccctgataa cccggcaatc gtcggcagca
    33121 gtgttctgac aaatgcaata aagcgaccaa ttaacagcgc cgataaaccg tgtttatgaa
    33181 aaagatggtg tgcgcgttga tgataatgcg cgggtaaatg agatagccag ttttgtacgg
    33241 tgcgggtatt gcccagccat cgcccctgaa tatagctgac ccagcaaccg aggctggcgg
    33301 caacggtcag cagcagaatc gtttgcgggt aacccatcgc gcctttcgca atcaacacgc
    33361 cgaccaatac cagtaaactg tcgcccggta aaaaggccgc cggaagcaag ccattttcaa
    33421 ggaacaaaat tacaaacaag acaaaataca acatgccaat catcgatgga ttggccaggg
    33481 tttcaaaatc ctgcgcccac agggcttgca gcaattgggt caaaagttcc attcattatt
    33541 cctggtgaat cggttaacgc cacgctggtt ttaaaagatg cactaccgca atgttattgc
    33601 tgttttggta tgttctgcgg gtttgataca tcaacacagc ctgaactatt ccctgttaaa
    33661 acgtgagata agaaagcatt caaaagcata aattcaaatt gaatctaatt gtaacaaagg
    33721 tcaacgttgt tcgttatcaa aattacatgt ccgtggagtc tgattttgct gattgcaggg
    33781 gagggtggcg agaggattta cacaggctga cactttatac ttttcgctta ccgacgagac
    33841 aaacaggcgc attgcagcgc ctgtcggaca ggaatttcat ttgctgccgc tggtggccgt
    33901 atcgagatgc actaccgggt tttcggcgaa cagatagcgg tcggcattga actcgaaatc
    33961 gtcgctggtt tcgttaaata acatgatttt ggtgttttcc agatgctgcc acattgccag
    34021 cttagcagca tgaggatctt tgcgaattaa cgccttgaga atttgatcgt ggtcatcaca
    34081 ccagttatca acggtacggg aatcaatgtg ttcgtgtagt tttttccagt acgggttatg
    34141 gctacgctgg gtccacattt tttcaacgat ggccgccagg gcggagttct gcgtcgccag
    34201 ggctacctga atgtggaact gtaaatccca ctcggaatca cggaagcatt gttcgccgcg
    34261 cgcctgttcc tgaatggcca tcagtttcat gatgtcttgt ttcgttacct gagttgccgc
    34321 gaactcggca atattacttt cgatgagctg gcgagcctga agcaactcaa acggaccgta
    34381 attggcgaat tccatattat tgtcagccgc ctgctgatga cgcggctggt tggaaaccac
    34441 atgaatacct gaacctttac gcacctccac atagccttca acttccagca tgatgatggc
    34501 ttcacgaact accgttcggc tgacgttctt ttcatcggca ataaagcgtt cggcaggcag
    34561 tttatcaccc accagataga cgccctgttc aatgcgctct ttcaggtcag cagcaagttg
    34621 ttgatacaaa cggcgtggtt cagtgatttc catatgcgtc tcccggagtg gaatgcggac
    34681 gaagttatca taccacttta ttcatgggta tgccattggt catatataaa tttgatgatt
    34741 gaattaatgt attaaattaa tattgtgaat atttataaat tcctaaaaaa taagtatcgt
    34801 cattgtgaaa gctactaaat ttcagtaagt taattcccag cctttaaatt taatgttaac
    34861 taaaaatgct tttttatatt gacttacaat aaatctacat ctctaataca ccaacttttt
    34921 tgaaacgata ttgtaagatc aaaattatat ggtggcgaaa attaatctct tattacggct
    34981 tacgccgctt ttattcgtgt tcgctccttt tttggcacag tcaaatatga ctgtttatcc
    35041 tatgcctgtt tccattaata gtcagggtga aggcaacgtt cgcgtaatat ctaaaagtaa
    35101 tgaagttcag tacatcaagg cgacagtatt ccgtatcgat aatccatcga cgcctcagga
    35161 aaatgaagtt gagattaaat ccggtgatgc aaatcatctg gttgttatgc cacctaaatt
    35221 cgctttacca gccggtagta gcaaaaccgt acgttttgtt gcgatggaac cagagcaaaa
    35281 agagaaaaat tatcgcgtta aatttgaagc ggttcccagt attgatgacg ttgccacaga
    35341 taaaaaagat ctctctatgc agttaacagt taacttaatt tggggaatcg ttgttagtgt
    35401 tccacctcag caacctatag ctaagttaga agtaaattct gcccaaaaat taattaatgc
    35461 agggaatcaa cgcttaaagc ttttaacgat cgcttattgt aaaaataata gcaaagaaaa
    35521 ctgtaagata cagaccgtaa ataaaaacat cttccctggt caggaaagaa atcttgaaag
    35581 catatctggc tacgacaaaa tcgttgtcaa atataataac tggatcacca aagataatgg
    35641 cgagtttgaa ctggcggtcc attaattatc attgtaataa acgaaggaat agtaatgaaa
    35701 aaggtatttg caaaatctct tctggtcgca gcgatgtttt ctgttgcagg ctccgcattg
    35761 gcagtacaaa aggatattac tgtaacggcc aacgttgatg ccgctctgga tatgacacag
    35821 accgataaca ccgcgttgcc gaaagcagta gaaatgcaat atctgccggg gcagggtctg
    35881 caatcttacc agttgatgac caaaatctgg tctaacgacg ttaccaaaga tgtaaaaatg
    35941 cagttagtgt ccccagcgca actggttcaa agcctggatg ctagcaagat tgttccgctg
    36001 acagtaacct ggggtggtga agaaattaaa gctgatgcgg caaccacttt taccgcaacc
    36061 aaaatctttg cttccgatgc gttaactaac ggttctctgg ctaaaaacct gatgtttgct
    36121 caaaccacca aaggtgttct ggaaacaggc atctaccgtg gtgtagtaag catttatctg
    36181 tctcaggata tttaatccgg ataacagaat taacacgtaa caaagggagg ggtagccctc
    36241 ccttatttta actgttttag tatttgttat ggatttcagg attgccatgg ataaaaaatt
    36301 actggctctt ttgatcctgg cgagtctcag cccggcagag gcgacattaa ccaaaattcc
    36361 cgcagggttt gaggttattg ctcagggaca gcaggagtat atcgaggttt atttttcagg
    36421 gaaaagtctc ggtaaatatt atgcaatggt taatcttgat accgtaacat ttcttgatcc
    36481 agcaagtcta tataacaagc tggaactgga tgtagacgat cagaaaatcg cgcatatagt
    36541 gaaagaaaaa ttatcgcagc cgctagctcg ccacggtgaa ttggcttgcg gttatgtacg
    36601 tactgactca gggtgtggtt ttctgaatac cgatacgctg gaaataatct ataatgatga
    36661 agaaagttcg gcaacgttgt ttattaatcc gcaatggaat tcagctttcg atgcgaagtc
    36721 attatattta aatccagaca aaaatactgt taacgctttt atacatcagc aagacatcaa
    36781 tgttctggca caggatgatt accaatcgtt gtctattcag ggaaacggtg cgctgggaat
    36841 aacagaaaat agctatattg gtgcacactg gaatttcaac ggttatgatg cagatgatgt
    36901 cagtgacagt aatactgacg tcagcgatct ctattatcgt tatgattttt tacgtcgtta
    36961 ttatgtgcag gcgggtcgca tggacaaccg cacactattt aatgcacaag gcgggaactt
    37021 tacctttaac tttctgccac tcggtgcaat cgatgggatg cgtatcgggt cgacactgag
    37081 ctatttaaac caggcgcaaa gccagcaagg aacccctgta atggttctgc tttcgcgcaa
    37141 ttctcgtgtt gacgcttatc gtaatgagca acttttggga tcgttttatc tcaatagtgg
    37201 ttcgcaattt attgatacca gttcctttcc gccaggcagc tacagcgtgg cattaaaagt
    37261 ctacgaaaat aaccaactca cccgcaccga gcttgtgccg tttaccaaaa ccggcggtct
    37321 gactgacgga aatgcgcaat ggttcttaca ggcaggtaaa actacatcac aggtttctga
    37381 tgatgaaagt tcagcttatc agctaggagt acgcctgcca ttacatccgc aatatgagct
    37441 ctacgcaggg ctggcgaatg ccgatgatgt gagtgctttc gagttaggta ataactggac
    37501 ggcagattta ggcggggtgg ggaatcttgc aatcagcgcc agcgtgttcc gtaacgatga
    37561 cggcggcaaa ggtgatatgc aacaggccaa ctggagtaat ccgggatggc cgacgttggg
    37621 cttttatcgg accaactctg acggcgatgc ttgtacaacc gacagcagag agagctataa
    37681 cgccttaagc tgttatgaaa gtatttccgc gacagtttca ctgaattttg tcggctggaa
    37741 tatgatgctg ggttatacct gcacacaaaa taacactgat gatagtttgc gttgggataa
    37801 acagcagagc tttgaaaata actatcttcg ccagacaaca gcgcaaagta tctctgaaac
    37861 tgtacaactt agcgcttccc gcgctattgt gatgcgtgac tggattttga gtacgtcagt
    37921 cggtgttttc catcgtaatg acaacggtgg cgataacgac gacaacggct tgtacttatc
    37981 gttttcgtta tctgacacgc caacgatgga tagcaataac aacagccatt caaccaatgt
    38041 gtctacggat tatcgttata gcgaacagga tggcgatcaa acgtcatggc agttatcgca
    38101 taccttttat aacgattcat tcagccataa agaacttggc gtaaccgtcg gaggcctgaa
    38161 caccgagacg atttgctggg tgcggtactg gtggatgtga aaggcttctc tgaacaggat
    38221 gaagagagtc aggatctgca actcgaagcg cgggtggcag gcagccgaac tttgcagctt
    38281 ggtcaaagtg acagtgtgtt gttcccttat cctggatttc agtctggttt tgttgaggtt
    38341 aacgacagta gccagggcaa tcagcagggg acaacaaaca tcattaacgg tgcagggaat
    38401 cgtgaattaa tgttgttgcc tggcaagctg cgctatcgcg aagtgtctgc cagctttaac
    38461 tacaactata tcggtcgttt attactgccg gcagcggtga aaaaattccc gattgtgggg
    38521 ctgaacagcg ccatgttact ggtagctgaa gatggcggat ttacacttga aattaacggc
    38581 agcgaaaaag agctctatct gctttccggg cagcaattcc ttaagtgtcc actgagtgtt
    38641 gtaaagaaac gcgccagcat tcgttacagc ggagatgtta cttgtagtgt ggtgacttat
    38701 tcacaattac cggagtcgat tcaggttcag gcacagttga aacagcctaa attacgtgga
    38761 aacgttcaga cggcgcaaag ggaggttgca ccatgagaaa tcgattgatt gcggcgatat
    38821 tgggcttgtt tggcacgctc actggcgttc aggcagctcc tgacgtgacc agtgaaatta
    38881 cgtatgattt ggcatctggt agagcggatt attacttctg gaaggatgaa gcgtcggcag
    38941 gaaataatgg atatatgtgg tatgaatgtt cgtatcctga cctccaacaa acctgtacag
    39001 ctaatggaaa tatatcgaca gtacaaatct atttaactga acaacgcagt gggatgcgtt
    39061 ggccggtaaa actcaaagga tttaaaacag ccattgtaag tagcgatgaa gcgccgctag
    39121 gatgcaaggg gggcaaaggg cttcagacga atcttaagga ttctaataga tcttcatgta
    39181 cagaagatgg tcaacattat tatatatacg atacaaagtt tcttacgctc taccttgagc
    39241 agacagagat gaagaatttg ccgattggtg gcgtctggaa ggggaaagtt aaattacatt
    39301 cgaacagccc ggcccaggac tatttcgcaa atattaccct gaatacgctc gaccccaacc
    39361 atattgacgt gttcttcccg gagttcgccc acgccacgcc aagggtgcag ttagacttgc
    39421 atccaacagg aagcgttaac ggcagcaact acgcgcaaga tctgaccatg ttggacatgt
    39481 gcctgtacga tggttttaac ggtaatgcca tcagttatga aatcatgctc aaagatgaag
    39541 ggcgaccagc tgcagggcgc agagacggtt acttctctat ctatcgtcag ggagggacca
    39601 ccaccgacga gggagaacgc attgattacc gggtcaaaat gtacaacccg gaaaccggtg
    39661 ggcaaattga tgtgcgcaat aatgaaaata tggtctggaa cagcattaac ctgaaacgtg
    39721 tgcgtccggt ggtactgccc ggtattcgct atgccgtgat gtgtgtgccg acacccttga
    39781 ctctggcagt cgataaattc agcgtgatgg ataaacaggc cgggtactac atgggcaaat
    39841 tgtcagtaat ctttacgcct tccttgccaa ccatcaatta accctgcaaa ccggacaaac
    39901 gcttttttct atataaacga cgaagccgcc tgataaggcg gcttcgtcgt tataacttac
    39961 gttaaacgtt aatgttgcgg tgcgggatcg ttatgtgttt cctgcgccac ctcaatcgcc
    40021 ggtttgttct gcaatacggt ccagataacc agcgcaccca gcaggtcaaa tactgccaga
    40081 actgcgaaca gcgggctgaa gccgatggtg tcagccagtg caccgacaac cagcgcaaac
    40141 agcgtacttg ccagccatgc ggacatcccg gttaaaccgt tcgccgtcgc cacttcgtta
    40201 cgaccgaaca cgtcagaaga gagcgtaatc agcgcgccag acagtgcctg gtgggcaaaa
    40261 ccaccgatac acagcagcat aattgcgaca tacgggttgg tgaacagacc gatcataccc
    40321 gggccaatca tcagcacagc acccagcgtt acgaccatct tacgggaaac gatcaggttc
    40381 acaccaaacc aacgctggaa cagcggcggc aggtaaccac cgaggataca accgaggtca
    40441 gcaaacagca tcggcatcca ggcgaacatc gcgatctctt tcaggttaaa gccgtaaact
    40501 ttaaacatga acagcgggat ccacgcgtta aaagtacccc aggccggttc tgctaggaaa
    40561 cgcggcagtg cgatacccca gaactgacgg ttacgcagga tctgaccaac ggacattttc
    40621 ttcgccgtgc tcacctggtg ctgggcttcc tgaccattaa taatatagtc gcgttcttca
    40681 tcggtcagat gcttctggtc gcgcggatgt ttatagaaaa tcagccatgc catcgcccag
    40741 ataaagctca atgcaccgga gatgataaat gccatctgcc agctgtgcat cacgattgcc
    40801 cataccacca gcggcggcgc aatcatcgca ccaatcgaag aacctacgtt aaagtagcct
    40861 actgcgatgg aacgctcttt cgccgggaac cattcggagc tggctttcag acccgccggg
    40921 atcatcgctg cttccgcggc accgaccgca ccacgagcaa cagccaggcc accccagcta
    40981 cctgccagcg cggttgcacc acagaacacg gcccacagca cagcaaacat tgcataaccg
    41041 attttcgtac ccagcacatc cagtacataa cctgctaccg gttgcatgac cgtataagca
    41101 gcagaatagg ctgcgatgat ataggaatac tgttgggtgg agatgtttaa ctcttccatc
    41161 agagttggcg cagctgccgc cacagtgtta cgcgtcaggt aaccaagcac ggtgccgagc
    41221 gtcaccagtg cgatcatata ccaacgtaac cctttaattt tacgcatgta aaacctcatc
    41281 gttttgttat atccgcagga agcgcggcca tcatcccgcc agaggctgaa gatgttagtt
    41341 acgaccgggt aaccgaaaaa aaaccggcac gccccgaacg ttgccatgaa agtttcgtgc
    41401 taattcggct tccgtaccgg ttaacccgat tcatctaaca aatgaatgaa tcggcaattt
    41461 gcattccgtc gacttatgat ttgcgacggc agaaagataa cttgtcatac aaccttaaaa
    41521 ggtgagagcc atcacaaatg tgcgaatatt tgtagggaca ttacctgacg acagcgaggc
    41581 cagtactggc gcggcctgca gcgagattta ccactttgag agtaattttt ttaactacgt
    41641 ttattgatct aactcacgaa aatatcttcg gactctggaa attggtgtga taactttgtc
    41701 agcatcgcac cataagcaag ctagctcact cgttgagagg aagacgaaaa tgactccgtt
    41761 tatgactgaa gatttcctgt tagataccga atttgcccgc cgtctgtatc acgactacgc
    41821 aaaagaccag ccgattttcg attaccattg ccatttgccg ccgcagcaga ttgcggaaga
    41881 ctatcgtttt aaaaacctgt atgacatctg gttgaagggc gatcactaca aatggcgcgc
    41941 tatgcgtacc aacggtgtgg ctgagcgtct gtgtactggt gatgcgtctg accgtgaaaa
    42001 atttgacgcc tgggcggcga ctgttccgca cactatcggc aacccgttat accactggac
    42061 gcacctcgaa ctgcgtcgtc cgttcggtat cactggcaaa ttgctttctc cgtcaactgc
    42121 cgatgaaatc tggaacgaat gtaacgaact gctggcgcag gataacttct cctcgcgcgg
    42181 catcatgcag cagatgaacg tgaaaatggt cggcaccacc gatgacccga tcgattctct
    42241 ggagcatcac gcagagatcg ccaaagacgg ctctttcacc atcaaagtgc tgccgagctg
    42301 gcgtccggac aaagccttca atatcgaaca ggcgaccttt aacgactaca tggcgaagct
    42361 gggcgaagtt tccgataccg acattcgccg ctttgctgac ctgcaaactg ccttgaccaa
    42421 acgtctggat cacttcgccg ctcacggctg taaagtgtct gaccatgcgc tggacgtggt
    42481 gatgtttgct gaagcgaacg aagcggaact cgacagcatt ctggcgcgcc gtctggctgg
    42541 cgaaaccctg agcgagcacg aagtggcaca gttcaaaact gcggtactgg tgttccttgg
    42601 tgccgaatat gcacgtcgcg gctgggtaca gcagtaccac attggcgcgc tgcgtaataa
    42661 caacctgcgt cagttcaaac tgctggggcc ggatgtaggc tttgactcca tcaacgaccg
    42721 tccgatggcg gaagagttgt ctaagctgct gagcaagcag aacgaagaaa acctgctgcc
    42781 gaaaaccatt ctctactgcc tgaacccgcg cgataacgaa gtgctgggca ccatgatcgg
    42841 taacttccag ggcgaaggta tgccgggcaa aatgcagttc ggttccggct ggtggtttaa
    42901 cgatcagaaa gacggtatgg aacgtcagat gacccaactg gcgcagctcg gtctgctgag
    42961 ccgttttgtc ggtatgctga ctgacagccg tagcttcctg tcatacaccc gtcacgaata
    43021 cttccgccgc attctgtgcc agatgatcgg tcgctgggtg gaagcgggcg aagcaccggc
    43081 ggacatcaac ctgctgggcg agatggtgaa aaatatttgc tttaacaatg cgcgtgacta
    43141 cttcgccatt gaactgaact aaggtctggg gttgatatgc aatacatcaa gatccatgcg
    43201 ctggataacg tcgcggtcgc tttagcagat ttggctgaag gcacagaagt cagtgtcgat
    43261 aaccagactg ttacgctgcg ccaggatgtt gctcgtggac ataaatttgc gttaacggat
    43321 atcgcaaaag gggccaacgt catcaaatat ggcctgccga ttggttatgc attggcggat
    43381 attgcggcgg gggaacacgt ccacgcccac aatacgcgca cgaatctgag cgatctggat
    43441 cagtatcgct atcaacctga ttttcaggat ctgcctgcgc aagcggcaga tcctgaagtg
    43501 cagatctatc gtcgcgctaa cggcgatgtc ggggtgcgta acgagctgtg gatcctgcca
    43561 accgtgggct gtgtcaacgg catcgcgcgg caaatccaga accgtttcct gaaagagacc
    43621 aacaacgccg aaggtaccga cggcgtgttc ctcttcagcc acacctacgg ctgctcacaa
    43681 ctgggcgacg atcacatcaa cacccgcacc atgctgcaaa acatggtgcg ccacccgaac
    43741 gcgggcgcag tgctggtgat tggtctgggc tgtgaaaaca accaggttgc cgcattccgt
    43801 gaaacgttgg gcgatatcga tcctgaacgc gttcatttca tgatctgcca acagcaggat
    43861 gatgagatcg aagccgggat cgagcatttg catcagctgt ataacgtgat gcgcaacgat
    43921 aaacgcgagc caggcaaact cagcgaactg aagtttggtc tggagtgcgg tggttctgac
    43981 ggtctttctg gtattactgc taacccgatg ctggggcgtt tctctgacta cgtgattgct
    44041 aacggcggta ctaccgtact gaccgaagtg ccggagatgt ttggcgcaga gcagttgctg
    44101 atggaccatt gccgcgacga agcaacgttt gaaaaactgg tcaccatggt caacgacttc
    44161 aaacagtact ttattgccca tgatcagccg atctacgaaa acccatcacc ggggaacaaa
    44221 gcgggcggta tcaccacgct ggaagacaaa tcacttggct gtacccagaa agcgggttcc
    44281 agtgtcgtgg ttgacgtgct gcgttacggc gagcgtctga aaacgccagg gctgaacttg
    44341 ttaagtgcgc cgggtaacga tgccgtagcg accagcgccc tggcgggtgc gggctgccat
    44401 atggtgctgt tcagtactgg tcgcggcacg ccatacggtg gatttgtgcc gacggtgaaa
    44461 atcgccacca acagtgaact ggcggcgaag aaaaaacact ggatcgactt tgacgcgggt
    44521 cagctgatcc acggtaaagc gatgccgcag ttgctggaag aatttatcga caccatcgtt
    44581 gagtttgcca acggtaagca aacctgcaac gagcgtaacg acttccgcga actggcgatc
    44641 ttcaaaagcg gcgtaacgct ataaagtcga aatgagttgt taataaaaaa cggcgtttca
    44701 taatgtgaag cgccgtttgc ttttccatac ctgctaagga tttatcatga ccgcgtattg
    44761 gctggcccag ggcgtgggtg tcatcgcctt tctgattggt atcacaacat ttttcaatcg
    44821 tgacgaacgt cgcttcaaaa agcagctttc ggtctatagc gccgttattg gcgtacattt
    44881 ttttcttctg ggcacctatc ccgctggtgc cagtgccatc cttaatgcca ttcgtacatt
    44941 gattacctta cgcacgcgca gcttatgggt aatggcgatt tttattgtgc tgactggcgg
    45001 aattggcctc gcgaagttcc atcatcctgt cgaactattg ccggttatcg gtacgattgt
    45061 cagtacctgg gcgctgttcc gctgtaaagg gctcaccatg cgctgcgtga tgtggttttc
    45121 aacgtgttgc tgggtgattc acaacttctg ggcggggtcg ataggcggca cgatgattga
    45181 gggtagtttt ctgcttatga atggcctgaa tatcattcgt ttctggcgga tgcagaaaag
    45241 gggaattgat ccgtttaaag tagagaaaac ccctcccgcc atagacgaaa ggggttaaac
    45301 aattaattac gcagggcgct atttgccaga cgatcgtctt ccgcctggca agctgccgca
    45361 gtgaacagca cgtcagttga agagttcagc gcggtttcgc aagagtcctg caatacgccg
    45421 ataataaagc cgacggcaac cacctgcatg gcgatatcgt tcgaaatacc gaacatatta
    45481 caggccagtg ggatcagcag caaagaaccc cccgccacgc cggatgcgcc acaggcacac
    45541 agagaagcca ctacgctcaa cagcagcgct gtgggcagat cgaccggaat acccagcgta
    45601 ttaaccgcag ccagcgtcaa cacggtaatg gtgattgctg cgcccgccat attgatggtg
    45661 gctcccagcg gaatagaaac tgaataggta tcgcgatcca gattcagctt ttcacacagc
    45721 gccatattca ccggaatgtt cgctgcagag ctgcgggtga agaaggcata cacaccgctt
    45781 tcgcgcaggc acagcagcac cagcgggaac gggttacgac gaattttcca ccacaccagc
    45841 aatgggttaa ccaccagcgc caccagtaac atacagccaa ccagcacgac cagcagttgc
    45901 gcgtagcccc acagtgtgga gaaaccggtg gttgccaggg tagaagaaac cagcccaaaa
    45961 ataccgatcg gtgcgaagcg aatgaccagt ttcaccataa aggtaacggc attcgacata
    46021 tcattgacca ggtttttggt ggtctcgtta ccgtgacgca gtgcgaagcc gaggccgatc
    46081 gcccacacca gaatcccgat gtagttacct ttcagcaggg cgtcgatggg gttggaaacc
    46141 atgctcatta ccagtccgcg catcacttcg acaatgcctg acggcggcga aatatcaccc
    46201 gcgctactgg acaagtgcag ggtagaaggg aaggcaaagc tgaagactac tgcggccaga
    46261 gctgcagaga aggtgcccag cagatagagg aacaaaatag gacggatatt ggttttctgc
    46321 ccgtgctggt ggttagcaat agatgccatt accagcatca acaccaggat gggggcaaca
    46381 gctttcagtg cgccgacgaa caaagtacct aacagaccaa cagcttccgc cgcgggtttt
    46441 gagatccatg ccagaagaat ccccagaaca aggccgacca ggatttgttt taccaggctg
    46501 ccatgagcca gacgccggaa tagccccggt gaacgttgcg tagtcatttt tcgatccttt
    46561 cattgtgttg tcgcacatcc ctggtgcgtt ctgtcgacgc atctttcagg atgtaacaaa
    46621 gtgtttgcaa ggtcgagtat aaggaaagtt acagcttcgg gaagcgaaaa atgctggatt
    46681 ttaaagagag gtcatgtttt gcaacttaat ggttgcattt atttaacata aacagaggga
    46741 aggactggct attaaggctg attagataac atatgggcaa aactatttgg aatatgagaa
    46801 cggttgcgct cctttaacgt gttatgccat ataaagtcac tggcgcaggg agcgcgcagg
    46861 gggcggccaa tcgccgccgc cccctgcacc ctcaggctct ggcgaacaaa atcgccgctg
    46921 cgcggttccc tcagcctatc ccttgcggct accgggtcgg gcgcaaggta acgtccctgt
    46981 aagatgcgcc ctcgccccgg cctccaggga acgcttcggc gattttgacg ccaccaacac
    47041 ccgtgcggtc tttttgtaaa gagctataag ccgattacat aattaatagc tcacctttgt
    47101 ctggaatgag ttatctctag gaatggctta atatacagcg aatcattctc cccgctgctt
    47161 atcatgccga taattcaccc aggcgttgat aataaacgtc atcaccagaa tgccaaacac
    47221 cacgcccagc gagacggcga ttggaatatg gtagaagtcg acaatcagca tcttgatacc
    47281 gataaacacc agaatcaccg ccaggccata tttgagcatc gagaaacgct ctgctacgcc
    47341 cgccagcagg aaatacatcg cacgcaggcc gaggattgca aacaggtttg aggtcagcac
    47401 aatgaacggg tcagtggtca cggcgaagat agccggaatg ctatccacgg caaaaatcac
    47461 gtcgctcaat tccaccagaa tcagtaccag catcagcggt gtggcgtaca gcaatccatt
    47521 cttacgcaca aagaaatgct cgttgtcgat ggtgtctgtc atgcgcaaat gaccgcgtag
    47581 ccagcgcacc agcggcttgt cgccaatgcc tgattcatct tcatgggcga gcgccatctt
    47641 cacgccggta aacagcagga aggcaccgaa gatatacagg atccagtcga actgcgaaat
    47701 cagccagctg cctgtgaaga tcatgatggt acgcagaaca atcgccccga gcacgccata
    47761 caccagcacg cgacgttgta atgccgccgg aacggagaaa tagctgaaca acatcagcca
    47821 gacaaagacg ttatcgaccg ccagcgattt ctcaatcaga taacccgtga gaaaggccag
    47881 ggcctgtgga tcggcaacgg cgcgaccttc ggtttgcacc agataccacc agaaagcggc
    47941 gttaaacagt aacgacagcg tcacccagac cagcgaccag gccgcagcct gtttcatggt
    48001 catggcatgt gccccacgac gcccctgcaa caacaggtcg atagccagca taatggcgac
    48061 aacaacagcg aatccgcccc atagcaacgg cgtgccgaca gtattcatag aagttcctta
    48121 cacataaaaa aacggccaac gtcggaagac gtcagccgct gctttttatg catagacctc
    48181 gccttccggc aaggtctcac ttacaacaaa aaaggaatac gtctgcgtat tcctttcggg
    48241 ttgcccggtg accggatgtg gtttttcaca catcgtaatg acgatcgacc ggcaatgaag
    48301 ttactcccct ttgcaggtaa caaagtatga cagaccattc agtcagtcaa tggtctgtta
    48361 catacctttt acactgtttt actttggcaa gtatagtttt acgctatctg ccggaaaaat
    48421 caccccagtc tggcggcgga tctcggtcag cagtttggct gtgatgcgac tgaccgccag
    48481 ccctggatga tccaccagat gctcatccac cagggtagcg aacagctctg cttcgtagag
    48541 catggtatta atatgctgcg gctgggtgag atcctgcatc tggctgccac gcggcacaaa
    48601 gcagactttc tggcattcag acagtttttc aatcaccagc gatcccgctt cgccctgaat
    48661 ctcgcttccc aggacagaat cactgacctt tgagtgctgc aaggtgacgc tgaaatcacc
    48721 gtaatccatc accaccacgc cgtgggcgtc aacgccgctt gccagcaaac tcgccgttgc
    48781 ctgcacgctt ttcggctcac caaataacgc caccgccgac gccaggcagt aaaagccgat
    48841 atccataatt gaaccgttag agaatgccgg attaaaggtg ttgggattct caccgtccag
    48901 gtaacgttga taccgcgagg aatactggca atagttgaaa aagactttac gcagtttgcc
    48961 tgctttcggc agcgcctggc gcaacaaatg aaagttcggc aggcaggcgg ttttaaatgc
    49021 ctcaaacagc accacctgat tttcgcgcgc acaggcaatg gcggcatcca cttccgccag
    49081 attcgacgcc agcggttttt cgcaaatcac atgaatttta tggctaagga aaagttgtgt
    49141 ctgggaaaag tgcagggaat tcgggctggc aatatacacc gcgtcaatgg catcgctttc
    49201 cgccatcgct tccagcgacg taaacagatg ctcgacagaa aaatcattgg cgaagtgctg
    49261 agcctgctca aggctgcggg aatatacggc ggttaacttg tatttaccgc tctcatgggc
    49321 ggcctcgacg aactggcgag tgatccagtt cgtaccaatc acagcgaaac gtatcataac
    49381 gcgtggatac tccataaagg gattctgtcg tcaatgtagc acgccttttt catcttcgac
    49441 gtgcgcagat acgcattatt ctttcgccgg acagccatgc tcactaaacg gcccaatcat
    49501 gaccgatggc ccgagatatt tcggctggcg atggaggatt tttgcgtcaa tcaccgcgct
    49561 gacacgagcg agtttctccc gcagttgagt ataaaaacca tgcttcatat tgaggtctgg
    49621 cgcgttaaac ccgatagcgt cgatgccgta ctgttttgcc agccagatgg cgcgttggtt
    49681 atgaaattct tgcgagataa tggtgatatg gttttcccca aacacctttt tggcacgcac
    49741 taccgaatcc agcgttgaga atccggcata gtcgcaaaag atcactttcg ccggtacgcc
    49801 tttagcgatc aaagcctgct gcatacctga tgcttcatcg taatttttac gtccgttatc
    49861 gccactcacc agtagccatt tcacttttcc ggcgtggtac aacgctgctg cggtatcaat
    49921 acgtcgggta aagtagcgat tacccggcct cgcccccagc aataaaccca cgttacgcgc
    49981 cgggacggcg ttgacatcgc cccaggtcag ctgcttgctg gcgttgacca taacgcgatc
    50041 ggcgataaaa atggtcgctc ctgccacaag taacagtaag caggcaatct ttagagtccg
    50101 gcgagaaaaa caaatacgga gaagaaggcg ggcaaatgcg cgcagcatgg tcgttatcct
    50161 taacgaaagg ggggaggtcg attttaggcg ctaaagaaag aaaagataac gcctgacatc
    50221 attttcagat tttgtcctgc ccaagcgaca actgcgttag ccacagccgg gtgtcgaact
    50281 caagctggtg gtactgcggc tccatatggc agcacaattg atagaacgct ttgttatgct
    50341 ctttctcctt caggtgcgcc agctcgtgca ccacgatcat gcgcaaaaac ggttccggcg
    50401 cgttgcgaaa cacggtagcg acgcggatct ctgcttttgc tttcagcttg ccgccctgca
    50461 tacgagaaac agcggtatgt aacccgagcg cgttttttag cacatggatc ttattgtcat
    50521 acatcacttt attgatcggc ggggcattac gcagaaactg atttttcaga tcctgagtat
    50581 attgccagag agctttatcg gtggcgtagt cgtgtgttcc cggatagcgt tttgccagca
    50641 catcgcccag acgctgttcg ttaatcagtg ttcgcacctg cgaaagcagc tgctcgggat
    50701 aaccctgaag ataagtaaga ttgctcataa ccgcccacga aaaagagaaa aagggtatac
    50761 tcacgcaccc ttttcagggg caacgtcgaa attttatcat tcaggagggc cgatgagcca
    50821 cttagacaac ggtttccgtt cactgacact acaacgtttt ccggcgacgg atgacgttaa
    50881 cccgctacag gcgtgggaag cggcagatga atatttgctg caacagttgg acgacacaga
    50941 aatccccggc ccggtgttga tcctgaatga tgcctttggt gcgttaagct gcgcgctggc
    51001 ggaacataag ccgtacagca ttggcgactc atacatcagt gaactggcga cgcgcgagaa
    51061 tttacgccta aacgggattg atgagtcgag cgtgaagttt ctcgacagca ccgccgacta
    51121 cccgcaacag ccgggtgtgg tgctgatcaa agtgccgaaa acactggcat tgctggaaca
    51181 gcaactgcgt gcgctgcgca aagtggtcac gtcggataca cgtattattg ccggtgccaa
    51241 agcccgtgac attcacactt ccacgctgga actgttcgaa aaagtgctcg gcccgaccac
    51301 caccacgctg gcatggaaaa aagcgcgcct gattaattgc acgttcaatg aaccgccgct
    51361 ggccgatgcg ccgcagaccg ttagctggaa gctggaaggt actgactgga ctatccataa
    51421 ccatgcgaat gtcttctccc gcaccgggct tgatatcggc gcgcgcttct ttatgcaaca
    51481 tctgccagag aatctcgaag gggagattgt cgatctcggt tgcggtaatg gcgttattgg
    51541 tctgacgctg cttgataaaa acccgcaggc gaaagtggtg tttgtcgatg aatcgccgat
    51601 ggcggttgct tccagccgtt tgaacgttga aaccaacatg ccagaggcgt tggatcgcag
    51661 cgagtttatg atcaacaacg cgctctccgg cgtggagcct ttccgcttta atgctgtgct
    51721 ctgcaaccct ccgtttcacc aacaacatgc gctgaccgat aacgtcgcct gggagatgtt
    51781 ccaccacgcc cgccgctgcc tgaaaatcaa cggcgagctg tatatcgttg ccaaccgcca
    51841 tctggattac ttccataaac tgaagaaaat tttcggcaac tgcaccacta ttgcgacgaa
    51901 taataaattt gtggtgctga aagcggtgaa gctgggacgt cgtcggtaag attgggagtt
    51961 acctgccgga tgcggcgtaa acgccttatc cggcctacgt tcagcacgtt acccccaggc
    52021 cggataagac gcggcaagcg tcgcatccgg caatgtctgc tcagttctgg aatcgcgctt
    52081 ccataaaaca aaaaatccct gtcaccatcc tctacattct cttgtttagc gtttttctac
    52141 gtttattctt ccgtcacaca gataaattca tccgttgcca atctgtcaac ggatgttatc
    52201 atattgccaa tttgtcaacg gacgtgatga agaatgcacc tgataactca aaaagcattg
    52261 aaagatgctg cggaaaaaca cccgcaacat aaaacggagt tggtggctct ggggaacacg
    52321 attgctaagg gatatttcaa aaaacctgag tcattaaaag cagtattccc atctctggat
    52381 aacttcaaat atctggataa gcattatgtt ttcaatgttg ggggcaatga attacgtgtt
    52441 gtagcaatgg tcttttttga atcgcaaaag tgctacatac gtgaagttat gacgcataaa
    52501 gaatacgatt tctttaccgc tgttcatcgt actaagggga aaaaatgatt gcgattgccg
    52561 acatcttgca agcaggagaa aagctaactg ctgtggcacc ttttctggcg ggtattcaga
    52621 acgaggaaca atacacccag gcgctggaac tggtagatca tctgctgctc aacgatcctg
    52681 aaaacccctt gctggatctg gtgtgtgcca aaataaccgc gtgggaagaa tcagcgcccg
    52741 aatttgcgga atttaatgcc atggctcaag ccatgcctgg cggtatcgcc gtgattcgta
    52801 cccttatgga tcaatatggt ttaacccttt ccgatctgcc ggaaattggc agtaaatcta
    52861 tggtgtcacg cgttttgagc gggaagagga aattaacgct ggaacacgct aaaaaattgg
    52921 caacgcgatt cggcatttct cccgccttgt ttattgatta acgcgacgtg ccggatgcgt
    52981 tgcatccggc gcttcaaata acgatttaaa tctccagcgc cagccgtgtt ccctgggcaa
    53041 ttgcccgtcg tgcgtccagc tccatagcca catcgcagcc gccaattaaa tgcacagttt
    53101 tcccgctatc aatcagcggt tgcgccagcg cgcggtttgg ctcttgccct gcgcagatca
    53161 ccacattgtc cactgctaat acctgcgttt cgccgttgat caccacatgc agcccgtcat
    53221 cgtcaatctt ctgataactt acgcctggga tcattttcac accccgcgag agcagggtgg
    53281 tgcgatggat ccagccggtg gttttgccta acccctgtcc cggtttgctg gctttgcgct
    53341 ggagcatcac aatttgccgt gggctacgcg ggatctgcat tccctgcggg cttaagccac
    53401 cagcctgttg taggctgctg tcgatccccc actcattaca gaacctggcg atattctggc
    53461 tggtggattc gcccggctga cttaaataca tcgccgtatc aaaaccaatc ccgccacaac
    53521 cgatgatggc aaccttgttg cctaccggcg ctttgtcgcg cagtacatcg agataactca
    53581 ataccttcgg atgatcgatc ccgtcgatag gcggaacacg cggcacgatc ccactggcga
    53641 ggatcgtttc atcgaacgcc tgtaactgat ccgccgtcac ggtgtgattg agtttcagcg
    53701 tcacgcccgt cacttcgatc atccggcggt agtagcgcag cgtttcgtaa aactcctctt
    53761 tgccggggat ctgtttggcg atattaaact gcccgccgat ctcgctgtgc gcatcaaaca
    53821 atgttacctg atgcccacgc gccgccgcgt taatggcaaa cgccagccca gcaggtccag
    53881 cgccgaccac cgccagattt tttttctgca cggcgggaag gattggcatt ttggtttcgt
    53941 ggcaggcgcg aggattcacc aggcacgagg tgactttgcc aacgaagatc tgatcgaggc
    54001 aggcctggtt gcagccaata caagtgttga tctcatcggc tcgccctgat tgcgcttttg
    54061 acagcagttc cgcatccgcg agaaacggtc gcgccatcga tactatatcg gcatcgccgc
    54121 gcgagagaat atcgtcggca acctgcggat cgttaatccg gttggtggtt accagctgca
    54181 gcgagacgtg gcctttcagt ttgcgcgtga cccagctaaa tgcgccgcgc ggcacgggcg
    54241 tggcgatggt cggaatccgc gcttcgtgcc agcctattcc ggtgttgata atggtcgcgc
    54301 ccgccgcttc aatggcctgc gccagttcta ccgtttcggc aaaagtgccg ccgccttcga
    54361 ccaggtcgag catcgacaac cggtaaataa taatgaagtc gttgccgacg cgttcgcgca
    54421 ccgtacgcac tacttctacg gcaaatcgca tccggttgcg gtaatcgccg ccccactggt
    54481 cactacgctg attggtgcgc agcgtcagaa attcgttgat caaataccct tcagagccca
    54541 tcacttcgac gccatcgtat ccggcctccc gcgccagttg cgcgcagcgg gcgaaatcgt
    54601 cgatcagttg caggatctct tcatgggtga gttcgtgagg aacgaaacgg ttgatggggg
    54661 cctgcaatgc ggacggtgcg accagatgcg gttggtagct gtagcgcccg gtatgcaaaa
    54721 tttgcagggc gattttgccg ccttcctgat gtacggcttc ggtaatggtg cgatggtgtg
    54781 ggatctggct ggcgtcgttg agcattgcgc caccttccat gccaacgcct attaaatctg
    54841 gtgcaatgcc gccgctgaca atcagcgcca cgccgtgacg ggcgcgttcg gcataaaacg
    54901 cagccagccg ttcagcaccg tccgggtatt cctccagccc ggtgtgcatt gaacccatca
    54961 acacgcggtt ttttaacgtg gtaaaaccta aatccagcgg ggcgaacagc gacgggtagc
    55021 tcataaaatt gtccagtatg taaaataatt gttatgtggt cggatgagtt ctaatttagc
    55081 cgtcgatgga gaaaagggaa aaggggagtg cggggtttgt gatgggattc aaaaagaata
    55141 cgcccggtgg taacgccgga tgcgacgctt gccgtgtttt atccggcttg ggggattgca
    55201 catgtagggc ggataaggcg ttcacgccgc atccgccagc cgttgcaaat tactgcttac
    55261 ggaaaaaatc gttatacaac atatacaaat gagcggcact ccaggagaaa tttggtgcgc
    55321 cttgctgcgc gcctgtcagc gggttgtaat tctcctgaat tgggccatcg gcggttaatc
    55381 ctttggcgtg cctgaagaac gtatccgcca gcttcagggc atcatcacga taaccgtaac
    55441 gctccatccc tttcagacca aaccagaact gatccaccca tacgcgcccg cgccagtaga
    55501 tatcagcgcc aaaagccgga ttggttaacg ccgccgttcc cagcggaaca aaggtgttga
    55561 actctttagg atcgagcatc accttcacca ccgcgtcggc attggcctgc gttgccgcac
    55621 cgttaaacag cggcgaccag ccttccggcc ctttaccgcg ctcaacaatc ggtttgcccg
    55681 cgcagccgtt cgccagtggt ttatcttcaa tacgtacgtc atagtagaac tgggtagtcg
    55741 ggtcgaacat acaggtgttg atgtagtccg cgagctgctg tgccaactgg cgatagcgtt
    55801 tggcctcttc tggcttaccg aggatggttg ccatctccgc cagataatgg ttatcgctgt
    55861 acatatagct ggcctgatcc accgactcct gcaatagcga gtagcccagc aacgttccgt
    55921 cctgactgcg gttttcggcg aatttcaccg tccagtcgct acgtttgccg ccattagcga
    55981 catatttatc caactgttct ttgtcgataa aaccaaagac ggcggcatca tcacgccccg
    56041 attcccacga cgcagcgacc tgtgccggaa tttccagact gtcgtactgg cctttctcca
    56101 ccacgcgggc gtagttgttc agcccagact gcgtctcttc tttatcgcct tttttcaccg
    56161 taaacagcat ctcgccgctc tcggtgttgt gggctttgtc gcgggtcgcg ccatattccg
    56221 gcaccccgtt gccgttatga tcgcggttac gtaaccacca gtcgtgatag gccaccagtt
    56281 tcgggtacat ctctgccaac caggctttat cctgggtgac gttgtatact tccatcaccg
    56341 accaggcggc aaggctgggt ttggtattac gttcgttcca gttgccgcca tcgccaccac
    56401 gctcggggct aaggttccac gctatcaggt cggggacaaa gcccacatcc tgcggacgca
    56461 cgctgtcgcc aggctggatc tgccaggaga agaccgcgcg gatattctct ttggcgatat
    56521 ccggattgaa atgcgccatc gcaaacgcct gcttccaggt atcccacggc caggtctgat
    56581 tgccggagaa ccagcgtccg gtcaccgacg gcgtgacggt gttataattc accgcaccgc
    56641 ccggtgagcg ccagttaccg ttgagcgttt cgatcgcttt caccgcgacg cgcgtctgtt
    56701 ccggcgtcgc atccggattg gttaaccctt tcttcaggta ttcttcccag cgttgctgcg
    56761 aggcggtgag ataaaacgcc ggacgcgcca gaatatcgcg gatctgcatt tgctctttgc
    56821 taacttcctg agcggtcagc agatgggaat aggtggtgta gagcgtggtc gaaccgttga
    56881 tatgcgcctt actggtaaag cgattaccgt tgatttcagt ctgcaccggc agggatttat
    56941 gcacctgata ttccgattcg ccggaggtca gcagatccca ggtggcgcgc actttgccaa
    57001 aggtgacttt caggccatca cgggtggcgc tgattttgcg ctgatagtca gggtattcgc
    57061 cagcaatggt tttatcggaa agcggtttcc cttctttcgc ttccagtttt tccagcagtt
    57121 cgccatccca caccagatcc agcggtttat tgctgatgat tttagtttcc agtagtgacg
    57181 tgcgcggcgt ggcgaagcgc agagtcattt cgacctgcac atcttttgct gtcagttttt
    57241 gcaccagcgc gccgggaata ctgtatgcct ccagcgtgaa gttgactttc ttgccgtctt
    57301 gccagacagt caggcggtcg aaattgctgg ccataaagtt gatgtattct tccgtcagca
    57361 gcgcaacgcc cggaaagccg cccatggtgt tcgggccgtc tggcaacaga tgaccatgcc
    57421 aggctccgag atcgaaaaac ggattaaaac gctggtgatc gtcgtaatcg taatccttca
    57481 tgtactgcgg cgcaccagta cggttaatca cgtttttata attgtcggcg ttagcggcat
    57541 gagcggaaaa acttatcagc agagcgcagg ttactggcgt taaaatagtt ttgattttca
    57601 tgaaattaca gtccttatta ccagtagaaa tattgcatca ggaagatttg gtcgctggga
    57661 tcgttagagg catttttcat gtagaagcgg ctatcaaaag ccgtggcgaa acgtccgccg
    57721 ccatattcgt accagatacc aaactgcatg aaagaggtgg tttcatcgtc agcgtggtca
    57781 gggcgatgtt tggcgtaggt gtaagcggtg gagagataaa cgccctttaa cgcttcatac
    57841 atggcgctca ccatcatccc gctttcactt cccggtacgt ccagcccgtc ggcacgtccg
    57901 gtatggtgcc aggcgcgagc ggcaaagtta ggggtaaaca ggcccgtgag atagagttgt
    57961 cctgtgccgt cggtaatttc cagaccattc atccaggtga tgccttcggc aaggtcatac
    58021 tggatgtaac cgttcatcca ttgccggata ggtgtatttg tcgtcgtagc gatcgtactt
    58081 gccaaaatgt agccaggctt tgctttcgtc atgatgtccg gcaggtgtgg cggtgacgct
    58141 ataacgaaag ttgccgctta agttctgtac cttcagcgcg gtcataatgt cgcgggtatt
    58201 gggaatgacg tagcccaaat cgggtgtgaa gtcgccccac cattgcaggt catccagcga
    58261 ggaatcctga cgcagactga gcatgatttc ggtgccatca ttggtacgat aaccgccata
    58321 gaaacggtta atcccacctt cgaaaccacc ccagctatgg tcaggcacct aggcattacc
    58381 gtcgtggtcg gcctgcacgg tccagccttc gccgtaaatc aggccgaacc aggagccgta
    58441 cttcatctcc atgccacctt caatataggt gccgtcgctg tatttatgtt tattctcacc
    58501 ctctaaatcc atatatccac caagccctaa ttcgccgtaa aaacgaaatg aaggagaaga
    58561 tgtcgaggta attgcgggtg tttttgtgtc gtcatgttct gccggacgtg cttcttctcc
    58621 tgccgcagaa aaggcgtagc aaaacggcag agcaagtaat gctctgcatg gtgcagtgat
    58681 taatttcatt gtgttaatcc atgagctaaa gaaaaagtac agccttttat cgttgtaatt
    58741 ataaaaatat aaaggcagaa actattttcc ctctcctcat ccaggaagag ggaaaatgcc
    58801 tgctcatttt ttattagtct gcttaatcaa cattgctggc tggcgtcgct tcaatatggc
    58861 gctcttcagc cgttaatccc tttatatatt tactgtattt ccaccacgca aagccgagga
    58921 agataacaat accgccgacg ttataaaaaa tgatggtcag aatattcgcg ccagtcggga
    58981 atgtcgaagc gacaaaccct acggcaaata tcgcaatcag cattgaaaca acgataatac
    59041 cggtgcggcg ggagcccata cggaaatcgc gtggcaggtg atctaatttg gcgcgtaaat
    59101 tcaggtaagc cagcatgatg aataacggcg gaagcatgga cgctgcggcg gtcatattaa
    59161 taatggtatt catcagatcc tgcacggtat tggaaccgag catcgggata atcatcagcg
    59221 ggatgacgat cagaaactgg atccatgctg cgcgcgccgg aacgccgttt tcgttaagtt
    59281 cgacggtttt cttaccaaag atgccttccg ggatttcgga gaagaaaatt ttcaccggcg
    59341 ttgcggtcca catcagcagg gaaccgaaca ttgcggtaaa ggacaccaga ccgacaaagc
    59401 gattcatcaa cgcttccggc agaccaaaat acgccgccat gccgtggaat acctgcaccg
    59461 atccgccggt aaatttcaac tctttgctgc tgacgaagac gttaatcagc accgaggaga
    59521 cggaatacag tacgccgata aaaatcccgg cgaggatgat cactttaacg aacgatttcg
    59581 aaccgccttt gacgtcgtta acgtacaccg cgacggactc cgcgccacct gcggccataa
    59641 agatccaggt ggtaacgccg aggaacgccc agttgaagtt cgggatcatc gcgtcaacgg
    59701 tgatcggatc ggcaggctgt acgtcgccaa ccagtgccgt acctgccagt aaaatgtagg
    59761 agagcgttaa cagcagcatc agcgttgaag tgacggaggt aattggcccc agcattttcg
    59821 ccccgttggt ggaaacccag gtggagaagg cgaacagcac catactgata acggtggtgg
    59881 caaccggcgt cataatatat tcgtagccga ggaaggcata cgaagcataa gcaataacgc
    59941 gcggcaacag cgaggtaaaa aagaacagat ttacgaacca gtaagtgtag gcagtaataa
    60001 atgcccaacg tccgcccagc gaacttttta cccacgcgta gacaccggct tctgagtttt
    60061 tatttaacga aacaaattct gcgatgatca gacagaaggg aataaaataa aaaatcgtcg
    60121 cgaggaaaaa catcggtgcc gaggccagtc caagctcaat attattattg ataacgttgt
    60181 taaagctgta aacggcggca aaagtcagcg agagcaagcc gaatttgccg attgtattac
    60241 gtttggtatc agacataaca catctccgaa aatgtgtagg gtagcggtgg cggcaaactc
    60301 cttttgccgc cgtagttgtt atttgttatg gaaataacca tcttcgatgg tgactttgag
    60361 aaccactttt ttgaccgcgt tattgcagat aaaccgatac gcttcatgga tatcgcaaat
    60421 aacgatttgc ccttcgtgga cctctacggt ttctccgcag ccttttaaat attcacggtc
    60481 agtttcatca cgataatatt ccactacctg taatgtctct ttcggcgcat attcaatttt
    60541 ttgctgccct tgcaggtagt aatgcacttc aaaatagcgt cgatgcccgg taaatagcgc
    60601 atcggtcgcg gagtcatttt ccacacggta ggtcaatgaa tcaccgatgg agtgggcgac
    60661 gccaggctga atgttgtcga tattttcaat cgcttcaacg cagcgttgcc acttcttgcc
    60721 agaggcgtaa atctggcgga actgttctaa gttatcgatg atcctcattg cttattctcg
    60781 ctgtgcaaat tcgtggaaaa gaaccctgcg ccgaactcat acgacgccag gctttgcgcg
    60841 gtagcttctc cgccagaaac cggcagcaac gtaaagccgt agctgaagtc acggaaccag
    60901 acgcgccagg agtccagcac ctcgctgccc caggagttgg agccgaggcc aagtagctgg
    60961 tggtcgaggt tcagggtgat gtcatcactg cgctgcagct cgttacagtg ctgggcagcg
    61021 tggatgtttt cctgggtata gtgccaggcg ctgaagttaa ttgggcgctg cggaaccacc
    61081 agcagaccgt tgtcgtggcg gttagtcagt gccgtccagc ggacatgctg acggttgccg
    61141 ttgttctgcg ggaagggata gttctcgaac atggcatcga cggtgctgcg ccagatatcg
    61201 atgatgttag cctgctggct gtcggcgtag ttttcgcccg gtccacgacc gtaatacgcc
    61261 acctgatcgt attcgccgtt aatccccatg gtgaaaccga tgcacggaat gatgtgcgga
    61321 tagtcgccgt aacgctcgcc ggaaagcgcc acgttaacct ggccatcggc agcgatgcgc
    61381 cagatgtagg tacagcgcat cccgaagtca aacaccggcg gtgcaataac tgtgcggttg
    61441 atgatcaaca cttcaccatc gctctgttct acggcaaagt cgcgcagatg ttcctgcatg
    61501 atctgcaaat gattcggctg ccacaaccct tcgtactcct gcttgtggtt gtcgatcatc
    61561 ggcttgaaga agttgatctt tggctcgcgg gtcagcagcg actcgccatt tacctgccag
    61621 gatgtcggtt tgccactcat ttttgagaag gtgatcgcga agttgtagcc gcgaacggtg
    61681 cagctcaaac gatcgtcttc cagcgtcagc ggacgcgcat tatttggtgc gaaaggcact
    61741 ggctgcgcgg tgttttcctt cagcgggaac tgataagtgg cgatagaatg cccggcttcg
    61801 ctgtagcggg tgcgggaatc tttggtcacc gtaatgttga ggaacgcttc gcgggcgtcc
    61861 agctgcggca gcgtgatctg caagggggct tcgctgttcg gcgcaacgtc gcgcagttta
    61921 atctgctgcg tcgcgagcgt ttcaccttcg gcgcgcacct ctgcgtgcag ggtgtagtca
    61981 tcaagcgtgg taaaccacag tttattttcg actttcaatt cgccgcgagt cagcgcgtgg
    62041 atttttaccg gcgcgataac ctgtttgtac tctttcaggc ccgatcccgg cgtctgatcg
    62101 gaatagatca aaccatcaag acagaagtta tagttgttgg gatagtcgcc gtagtcgccg
    62161 ccgaatttat accagacatt gccgtggtcg tcctgtgcct ggatcccgtg gtcgcaccac
    62221 tcccagacat aatgaccctg aatgcaatcg tgcttataga agacgttctg gtactccgtc
    62281 agcccgcccg gtccgttccc catcgcatga gcatattcac agatgatgcg cggcttcgga
    62341 tgcgggtatt caccaaactc attcatcagc ggcacgcggg tgtacatggt ggaaataata
    62401 tcgaccactt cagcatcgcg atcttcttcg taatgcacca gtcgcgtgtc atccagcgct
    62461 ttcgccgcat ggtacatcgc gcggatgtta cagccatagc cggattcatt gcccagcgac
    62521 cagatgatga tcgacggatg gtttttctgt gcgtggatat ggcgaacaat gcgctcgacg
    62581 tagacttttt cccactgcgg atcgtcggta atacggctga tatcgccgac attagcaaag
    62641 ccgtgcgatt cgacgtcggt ttccgccatc acaaacaggc cgtagatatc acacaattcg
    62701 taaaaacgcg gatcgttcgg gtagtaagcg gtacgcacgg agttgatatt gtgctgcttc
    62761 atcaactgga gatctttctc gacgcgatcc attccaacgg cgcggccttt gcgatgatcg
    62821 ttgtcgtgac ggttgacgcc gtgcagcatc acataacggt tattgatcca gaacagaccg
    62881 tcgcgcactt tgatatcacg gaagccaacg cgttgtggca ccacttccag aacgttgccg
    62941 ttggcgtctt tcagcgtcat gaccagatgg taaagataag gggattctgc tgaccattgc
    63001 tgcggctgtt cgacagtaaa agcaaagctg gcgctggtca gtttttcaat tgccaaatga
    63061 tcaatggcgc tgctgtgcac cacgcgttcg ccatcaaaca gggtatattc cagcgtcgtg
    63121 acgacagggg aggcggcgag attttccagc accacttcgc aggaaagcgt ggcatcgcaa
    63181 taggcttcgt caaagtcggt acgcacagtg aaatcgttaa tatgcgttag gtgttttccg
    63241 accagataaa catcgcggaa gatccccgcc gaccaccaca tatcctggtc ttccacgtag
    63301 gtagagtccg cccactgcat cacgcgcaca cacaacaggt tgtcgccggt tttaaccatc
    63361 gcgctgatgt caaactctgc ggtcaggcga ctgcccttgc tgaaacccac atactgaccg
    63421 ttaacgtaga cttcaaaata ggtttcgacg ccgtcaaatt taatcagcgt ctgtttaccc
    63481 tgccagccgt cgctgagggt gaaaatacgt tgataggcac cggttgggtt atcgctgggg
    63541 acaaacggca catcgatggg gaacggaaaa ccttcgtcgg tatattgcag tttgccgtga
    63601 ccttccattt gccacatggc ggggacggta atatgccccc agtcagccat taactcagag
    63661 gtgaaggctt ctggtacttg cagcggatgg tcaaaaaagt ggaaattcca ctgaccgctt
    63721 aagggcagaa acaggctgct ggtttcgcgg gcaaaggtac gcgcttgcgc aacagaatca
    63781 tatgaaaaaa agtacgcacg cggcgcaagt cggttttcgt gggtgagctg aatgttttcc
    63841 cagcgattca tagggcctcc cggtagagag aaatcggaac cattcatggt gatggtagtt
    63901 tgtgtaattg ctgggggttt agtaaaattt ttactaaaag tagcaggtaa atgcatttac
    63961 tttaactgga tcacgaaaat aaacggcagc actgtccggc aaaaatgatt gccggacagt
    64021 gtgtaagggg atttaacggg tcgtgccgcg cagttttaat ttgctgggaa cgaagactag
    64081 cagcggcagc gcgcgaccat cgcgggcttt ttcatacacc aggttaacgc cctgacttcc
    64141 catcatctcg gaatggatgc gcacggtgga gagcggcgga aaggtaaatc gcgcggtggg
    64201 gatatcgtta acgctgataa gcgaaatatc ttgtgggatg ttcaggccac gttcatgaat
    64261 tgcccgcagt acgccgatag caatggaatc ggaagcaaca aacagtgcct tcggatagtc
    64321 ttcccgcgcc agcatttgtt ttgccagttc ataacccgac gaactggaaa aaccgccgcg
    64381 ccagatatcc tcttcgcgta ccacttgttt cagtcggcca tattccgcaa aggcgacctc
    64441 acgaatatcc gccttgccag gctcatcttc accgccaata aaaccaatac gattaacgcc
    64501 ctggttgata tagaagtcga tgatttcttt actgatgcgt gccagatcga tatccaccgc
    64561 atcgtaaccg ctgccgggtt cgtgaaagtc gataaaacag atattgtcgg tcaacgcgct
    64621 ggcagcggcg cgcagggcgg gcgtgggttt gccgacaatt aaaataccgg tgacgttttt
    64681 aatgtctggt aatccgttgt gttcataaca gttgttcagt tcgatcgcca gcttttcgca
    64741 ctgagtttca atgccgtggc ggatcgccag atagtaagga tcgttgatct ccagctcctg
    64801 ctggtagctg tagatagcca gaatatggtg ttggttgact gcgcctgtct gtagtttacg
    64861 ggcgctactg gtcttgtact ccagcttttc ggcgatctcg agaatgcgat gtttcgtctc
    64921 ttctttcaca ttcaatgtcg gatcgtcatt taagaccctg gatactgtcg ccagggatac
    64981 gccagcttcg attgcgatgt cttttaatgt tgccatgttt acctttttta ctttccctga
    65041 tgtgcgcccc cattgtagag gaagtgctta tcgggtcacc tgttttcagt aaataaagcg
    65101 cgtttctttg acgctgtaac ggtaacacag cggcttagga atgtgatcac gcctgcattc
    65161 tccattttta gcctacagga ttgagaaaaa cttttgctat agtttagtaa aattttaccc
    65221 gaagggagga agcaatggaa accgtggctt acgctgattt tgcacgtctg gaaatgcgcg
    65281 tcggaaagat tgtggaagtg aaacgccatg aaaacgccga caagctgtac atcgtacagg
    65341 ttgatgtggg gaaaaaacac tgcaaaccgt gaccagcttg gtgccgtact acagcgaaga
    65401 ggagctgatg gggaaaacgg tggtggtctt atgcaatctg caaaaagcga aaatgcgtgg
    65461 tgaaacgtcg gaatgcatgc tgttgtgtgc ggaaaccgat gacggcagtg aaagcgtgtt
    65521 gttaacgccg gagcggatga tgcctgcggg cgtacgcgtc gtataaaaag atcggatggc
    65581 gacgtcgtat cgccatccga tttgatatta cgcttcttcg acacttactc gcatggccgc
    65641 cagcgcctta cgcgccgctt tgatcaccag ttcacactgt tcaatggtca gtgtcagtgg
    65701 cggttcaatg cggatcgttt tggcgttatt gagcgttccg gccaccagta cgcgctggcg
    65761 gaacatctcg ctggcaaagt tatagccgat ttcgttatca acaaactcaa tcgccatcaa
    65821 catcccttta ccacgcgctt cctgtaccag atcgggatat tcccgcgcca gttgacggaa
    65881 accgtccagc aacacattga tggtcgccag cgccgccgca caggccagcg ggttgccgcc
    65941 aaaggtggtg gtatgcagga atgggttgtc gaacagaact gaaaacacct cttcagtggc
    66001 gatggtcgcg ccaatcggca tcacgccgcc gccgagggct tttgccagac acagaatgtc
    66061 cggctgaacg ttctcatgtt cgcaggcaaa catcttgccc gtgcgcccca tgcccgtttg
    66121 tacttcatcg aggatcatca gtgcgccgaa ctcatcgcac agcttacgca cggcggtgag
    66181 atagcccggc ggcggcagaa ttacgccacc ttcgccctga atcggttcga ggatcaccgc
    66241 agccacatca tcaccggttt ttttgcactc attaagagcc gtgcgcatgg cttcgatatt
    66301 gccaaacggc acatgacgga accctggcag cagtggcata aacggtttgc ggaaggtcga
    66361 tttcgccgtg gccgacagcg cgccaagtga tttaccgtgg aacgcgccgc tggtggcaat
    66421 aaaagtaaat ttgccgcgcg gcgactgata agcctttgcc agcttcagcg ccgcttcaac
    66481 ggactcggtg ccgctattac agaagaagct gtatttcagt ttaccgggcg ttagcgcagc
    66541 aagggttttc gccagcatag cccgtagcgg atcgagtagc tcctggctgt gcagtggttg
    66601 tttcgcaagt tgattctgta cggcggaaac cacaactgga ttacggtgcc ccacgttgaa
    66661 aattccaaaa cctcccaggc agtcgataaa ctcctgtccc tgggtgtcga caagcgtatt
    66721 taaacctccc gcttgccact ctacggctcc gtaatccccg ccggcggtaa cagatttgcg
    66781 atactctaaa aaccccggat tgacatgctc tttgaagtat tcaatcacct ctcggttaag
    66841 tgctttcatc tcctcatgat ccagcgttcg cttctcaatg agattcaggg cgtgggcgct
    66901 gcacgctaaa gccgatgcgc tcgaaggtaa cctgttcaaa atatgctccg gaggctcgcg
    66961 tatcacatga taccgattta agtattgcag ggattgcgcc actccggctg cgatcgcgaa
    67021 aatcgggata aacacaaatg cggttatcat ttgtgcaata tttaatgcta tccgttaaca
    67081 tttgttgtta ttttgcacgg ttttgtcggg tatggatttg atgattgctg cgcaaatgca
    67141 cgttttcagg gcaataattg cctgattttt gggcaagata attaacgttt tcgtatagta
    67201 acagagtgaa agtattttaa atcaaagaat tagatttgtg ctttaatcgc aaattgcgat
    67261 ctaaatcaaa ttaatcggtt aaagataacc gcagcggggc cgacataaac tctgacaaga
    67321 agttaacaac catataacct gcacaggacg cgaacatgtc ttctcatccg tatgtcaccc
    67381 agcaaaatac cccgctggcg gacgatacca ctctgatgtt cactaccgat ctgcaaagct
    67441 atatcactca tgctaatgac acttttgtgc aggtgagcgg ctttaccttg caagagttac
    67501 aagggcagcc gcacaacatg gtgcgtcacc cggatatgcc aaaagcggcg tttgcggata
    67561 tgtggttcac cctgaaaaaa ggggagccct ggagcggcat cgtgaaaaat cgccgcaaaa
    67621 atggcgacca ttattgggtg cgggccaatg cggtaccgat ggtgcgcgag ggaaaaatca
    67681 gtggctatat gtcgattcgt acccgggcga cggatgaaga gattgcggcg gtggagccgc
    67741 tgtacaaagc gttgaactcc ggacgtacca gtaagcgtat tcataaaggc ctggtggtgc
    67801 gtaaaggctg gctgggtaaa ctgccttcat taccgcttcg ctggcggacg cgtggagtga
    67861 tgaccctgat gtttatcttg ctggcggcca tgctttggtt tgttgctgcc ccggtggtga
    67921 cgtatttcct ctgtgtgtta gtggtattgt tggcaagcgc ttgttttgaa tggcagattg
    67981 tgcgcccgat agaaaatgtc gcccgtcagg cactgaaggt ggcgaccgga gagcgtaata
    68041 gtgttgagca cctgaatcgc agcgatgagc tggggctgac attacgcgcg gtagggcagc
    68101 ttggcctgat gtgccgttgg ctaattaacg atgtctcaag ccaggtgtcc agtgtcagaa
    68161 atggcagtga gacgctggcg aaaggcaccg atgaactgaa cgaacatacc cagcagacag
    68221 ttgataacgt tcagcaaacg gtggcgacca tgaaccaaat ggcggcgtcg gtgaaacaga
    68281 actctgccac ggcgtcggct gccgataaac tttctatcac cgccagtaat gcggcagtgc
    68341 agggtgggga ggcgatgacc acggtgatca agacaatgga cgatatcgcc gacagtaccc
    68401 agcgcattgg caccattact tcgctgatta acgatattgc gtttcagacc aatattctgg
    68461 ccctgaatgc ggcggtggaa gcggcgcgtg ccggcgaaca gggcaaaggt tttgcagtgg
    68521 tggcagggga agtgcgtcat ttagccagcc gcagcgctaa tgctgccaac gatattcgca
    68581 agctgattga tgccagtgct gataaggtgc aatccggttc gcagcaggta cacgccgccg
    68641 gacggacgat ggaagatatt gtggcacagg tgaaaaacgt cacccagttg atcgcccaga
    68701 ttagccattc aacgctggaa caggccgatg ggctttccag cctgacccgt gcagtggatg
    68761 agcttaacct gatcacccag aaaaatgccg agctggtgga agagagtgcg caggtgtcgg
    68821 cgatggtgaa acaccgcgcc agccgactgg aagacgcggt gacggtgctg cattaatcgg
    68881 tatgccggat ccggcggttg gagcacaata cctgatgcga tgctggcgca tcttatcagg
    68941 cctgcggggt gtgtagcggg tgtaggccgg ataaggcgcg tcaagcgtcg catccggcaa
    69001 ttgcaccgcg ccactggcgg atgcggcgat taatccagtt gcgtaatatc aaaagcggcg
    69061 cggtcgatca ccgcaatgat cttttttatt tgtgcatcac tgatatcgct ctggttgacg
    69121 cgtaaatcca gcaccgcttt aaaattatcc agcgcccgct tcatttgcgg gttctggcgc
    69181 agcgccgcgc caacgcaacg cgctttgatg cgttcttcaa tcatctccac ctgttcgcgg
    69241 ttttcttcca gccactgcgc gccttgttcg gtcagcgcaa tctgcttctt accaccttcc
    69301 tcttcgcgga tggtaatcag cgactgctcc tgcagaaaat ccagcgtcgg gtagatgacg
    69361 cccgggcttg gggtgtaatt cccctgggtt agattctcaa tcgctttaat caattcgtaa
    69421 ccgtggctgt catcgcgcga gagaatatcc agaatcacca gacgcaattc accgtgacca
    69481 aagaaacgtt gccgacgacc gccgccgcga ccgtggcgac caccgcaaca ttgaccgtgt
    69541 tcatgctggt gaccgtgtcc gcagtgctcg tgttctgact tctcaccttt gcagcagccc
    69601 tcatggcgtg gctggccttc atgtttacaa cacccttcgt gatgatggct catatcagcc
    69661 tccttttgct tgattagata tatctaattt atatctgatg tgagcattac tgcaagtaca
    69721 aaaatgattt ttaaacgtgg aagcacctca catctaggcc ggataagacg ttcactccgc
    69781 atccggcagc attgctatgg cactgaatgc cttcacatac ttcagacata tcagttctac
    69841 aaatcgcttg catttatcat gattaacaat cattatcatt tgcgaatttt atttagatat
    69901 atctgattac catcacgaag gcgataacaa tgaataacac cccccgctac ccgcagcgcg
    69961 ttcgcaatga tctgcgcttc cgtgaactga ctgtgctacg cgttgagcgc atcagcgccg
    70021 gttttcagcg cattgtcctt ggcggcgagg cgttggacgg ttttacatcg cgcggctttg
    70081 acgatcacag caaactcttc tttcctcaac ctgacgctca ctttgtgccg ccaacggtaa
    70141 cggaagaggg catcgtctgg ccggaaggac cacgcccacc gtcgcgtgac tatacgccac
    70201 tgtatgacga actacgccat gaactggcga tcgatttctt tattcacgac ggtggggtcg
    70261 ccagcggctg ggcgataaac ttacggtggc aggtccgcgc ggttcgctgg tggtgccgga
    70321 agattacgcg tatcagctgt atgtctgcga tgaatccgga atgcccgcac tgcgccgccg
    70381 cctggaaacg ttgagcaaac ttgccgttaa accgcaagtt agtgcgctgg ttagcgtgcg
    70441 ggataacgcc tgtcaggatt atctcgcgca ccttgatggt tttaatatcg aatggctggc
    70501 acatgatgag caggcggtag atgcgcgtct ggcgcagatg caaatccctg ccagatgatt
    70561 acttcatctg gataaccggc gaaggtaaag tcgttaagaa tttaagccgc cgctttgaag
    70621 cggaacagta tgacccacag cgggtacgtg cagcggctta ctggcacgca aagtaactga
    70681 tatataaaaa gaaaaaggct gacgatttct cgtcagcctt tgctatatct ggtggcccct
    70741 gctggacttg aaccagcgac caagcgatta tgagtcgcct gctctaacca ctgagctaag
    70801 gggccatggt agcggattat aaagtaactc cgtgtcgcaa tccagccatt accgcgcgcc
    70861 tgctgttttt ataaacaatg tattttcaat ccgttatact tttcctggtg atgttagaaa
    70921 ggagtaaata tggttgagga tattttggct ccagggttac gggtcgtgtt ttgcggtatc
    70981 aaccctgggc tttcatccgc cgggactggt tttccctttg ctcatccggc aaatcgcttc
    71041 tggaaggtga tatatcaggc cgggtttacc gaccgtcagt tgaagccgca ggaggcacag
    71101 catctgctgg attatcgttg tggcgtcacc aaactggtag accgtccaac ggtgcaagcc
    71161 aatgaagttt caaagcagga gctgcacgca ggtgggcgta agctgattga aaaaattgaa
    71221 gattatcagc cgcaggcgtt ggcgattctg ggcaaacaag catatgaaca gggattcagc
    71281 cagcgcggtg cacagtgggg gaaacaaacg ctcaccattg gttcgacgca gatttgggtg
    71341 ctgccaaatc ccagcggttt aagtcgcgtt tcactggaga aactggttga agcgtatcgc
    71401 gagctggacc aggcgctggt agtgcgtggg cgataaaaaa cgccaccgaa aaggtgtaat
    71461 gccagtcagt taagcaactg actggctctt tttcggggct gtggggtatt tccagggcct
    71521 ctcctttacc actctcggga aggcccttcc ccttctcgtc ggtaatttca caagttgtcc
    71581 catacttgca agatcgcgca tcagctccgg
//
LOCUS       DAETPZ010000006        69289 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.006, whole genome shotgun sequence.
ACCESSION   DAETPZ010000006 DAETPZ010000000
VERSION     DAETPZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 69289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.006"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..188
                     /locus_tag="K0W59_000490"
     CDS             <1..188
                     /locus_tag="K0W59_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3021655.1"
                     /translation="GIRIENLGGGVANIPFQNGILPVDPSGHGTVNMRAWPVNLVGGE
                     LETGKFQGTATITVMVR"
     gene            complement(231..1091)
                     /gene="rsmI"
                     /locus_tag="K0W59_000491"
     CDS             complement(231..1091)
                     /gene="rsmI"
                     /locus_tag="K0W59_000491"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBI3021656.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1156..3192
                     /gene="lpoA"
                     /locus_tag="K0W59_000492"
     CDS             1156..3192
                     /gene="lpoA"
                     /locus_tag="K0W59_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HBI3021657.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLSVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3150..3545
                     /locus_tag="K0W59_000493"
     CDS             3150..3545
                     /locus_tag="K0W59_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBI3021658.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            3565..4155
                     /gene="diaA"
                     /locus_tag="K0W59_000494"
     CDS             3565..4155
                     /gene="diaA"
                     /locus_tag="K0W59_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HBI3021659.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4165..4740
                     /gene="yraP"
                     /locus_tag="K0W59_000495"
     CDS             4165..4740
                     /gene="yraP"
                     /locus_tag="K0W59_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HBI3021660.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(4854..5894)
                     /locus_tag="K0W59_000496"
     CDS             complement(4854..5894)
                     /locus_tag="K0W59_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBI3021661.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(5967..6602)
                     /locus_tag="K0W59_000497"
     CDS             complement(5967..6602)
                     /locus_tag="K0W59_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBI3021662.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6730..7248
                     /gene="yhbO"
                     /locus_tag="K0W59_000498"
     CDS             6730..7248
                     /gene="yhbO"
                     /locus_tag="K0W59_000498"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HBI3021663.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTSAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7228..7671)
                     /locus_tag="K0W59_000499"
     CDS             complement(7228..7671)
                     /locus_tag="K0W59_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HBI3021664.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            7722..8024
                     /gene="yhbQ"
                     /locus_tag="K0W59_000500"
     CDS             7722..8024
                     /gene="yhbQ"
                     /locus_tag="K0W59_000500"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HBI3021665.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGVAELLSSLQTPEIKSD"
     gene            complement(8011..8514)
                     /locus_tag="K0W59_000501"
     CDS             complement(8011..8514)
                     /locus_tag="K0W59_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBI3021666.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8508..9032)
                     /locus_tag="K0W59_000502"
     CDS             complement(8508..9032)
                     /locus_tag="K0W59_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HBI3021667.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9241..10236
                     /locus_tag="K0W59_000503"
     CDS             9241..10236
                     /locus_tag="K0W59_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBI3021668.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHISINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10245..11123
                     /locus_tag="K0W59_000504"
     CDS             10245..11123
                     /locus_tag="K0W59_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBI3021669.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            11204..12211
                     /locus_tag="K0W59_000505"
     CDS             11204..12211
                     /locus_tag="K0W59_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBI3021670.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSIQQAFVKLRRGEIRQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12329..13573)
                     /gene="mtr"
                     /locus_tag="K0W59_000506"
     CDS             complement(12329..13573)
                     /gene="mtr"
                     /locus_tag="K0W59_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HBI3021671.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13727..15616)
                     /gene="deaD"
                     /locus_tag="K0W59_000507"
     CDS             complement(13727..15616)
                     /gene="deaD"
                     /locus_tag="K0W59_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HBI3021672.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGELRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(15609..15689)
                     /gene="yrbN"
                     /locus_tag="K0W59_000508"
     CDS             complement(15609..15689)
                     /gene="yrbN"
                     /locus_tag="K0W59_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HBI3021673.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(15796..16680)
                     /gene="nlpI"
                     /locus_tag="K0W59_000509"
     CDS             complement(15796..16680)
                     /gene="nlpI"
                     /locus_tag="K0W59_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HBI3021674.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(16789..18924)
                     /gene="pnp"
                     /locus_tag="K0W59_000510"
     CDS             complement(16789..18924)
                     /gene="pnp"
                     /locus_tag="K0W59_000510"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBI3021675.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19171..19440)
                     /gene="rpsO"
                     /locus_tag="K0W59_000511"
     CDS             complement(19171..19440)
                     /gene="rpsO"
                     /locus_tag="K0W59_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBI3021676.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(19589..20533)
                     /gene="truB"
                     /locus_tag="K0W59_000512"
     CDS             complement(19589..20533)
                     /gene="truB"
                     /locus_tag="K0W59_000512"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBI3021677.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(20533..20934)
                     /gene="rbfA"
                     /locus_tag="K0W59_000513"
     CDS             complement(20533..20934)
                     /gene="rbfA"
                     /locus_tag="K0W59_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBI3021678.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21098..23746)
                     /gene="infB"
                     /locus_tag="K0W59_000514"
     CDS             complement(21098..23746)
                     /gene="infB"
                     /locus_tag="K0W59_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBI3021679.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(23771..25258)
                     /gene="nusA"
                     /locus_tag="K0W59_000515"
     CDS             complement(23771..25258)
                     /gene="nusA"
                     /locus_tag="K0W59_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBI3021680.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(25286..25738)
                     /gene="rimP"
                     /locus_tag="K0W59_000516"
     CDS             complement(25286..25738)
                     /gene="rimP"
                     /locus_tag="K0W59_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBI3021681.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(25945..26021)
                     /locus_tag="K0W59_000517"
     tRNA            complement(25945..26021)
                     /locus_tag="K0W59_000517"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25985..25987),aa:Met,seq:cat)
     gene            26369..27712
                     /gene="argG"
                     /locus_tag="K0W59_000518"
     CDS             26369..27712
                     /gene="argG"
                     /locus_tag="K0W59_000518"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBI3021682.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(27720..29327)
                     /locus_tag="K0W59_000519"
                     /pseudo
     CDS             complement(27720..29327)
                     /locus_tag="K0W59_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(29805..29891)
                     /locus_tag="K0W59_000520"
     tRNA            complement(29805..29891)
                     /locus_tag="K0W59_000520"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29855..29857),aa:Leu,seq:gag)
     gene            complement(29906..30238)
                     /gene="secG"
                     /locus_tag="K0W59_000521"
     CDS             complement(29906..30238)
                     /gene="secG"
                     /locus_tag="K0W59_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBI3021683.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSTPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(30466..31803)
                     /gene="glmM"
                     /locus_tag="K0W59_000522"
     CDS             complement(30466..31803)
                     /gene="glmM"
                     /locus_tag="K0W59_000522"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBI3021684.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(31796..32644)
                     /gene="folP"
                     /locus_tag="K0W59_000523"
     CDS             complement(31796..32644)
                     /gene="folP"
                     /locus_tag="K0W59_000523"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBI3021685.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(32734..34668)
                     /gene="ftsH"
                     /locus_tag="K0W59_000524"
     CDS             complement(32734..34668)
                     /gene="ftsH"
                     /locus_tag="K0W59_000524"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBI3021686.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(34768..35397)
                     /gene="rlmE"
                     /locus_tag="K0W59_000525"
     CDS             complement(34768..35397)
                     /gene="rlmE"
                     /locus_tag="K0W59_000525"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HBI3021687.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            35523..35816
                     /gene="yhbY"
                     /locus_tag="K0W59_000526"
     CDS             35523..35816
                     /gene="yhbY"
                     /locus_tag="K0W59_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBI3021688.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(35972..36448)
                     /gene="greA"
                     /locus_tag="K0W59_000527"
     CDS             complement(35972..36448)
                     /gene="greA"
                     /locus_tag="K0W59_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBI3021689.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            36696..38129
                     /gene="dacB"
                     /locus_tag="K0W59_000528"
     CDS             36696..38129
                     /gene="dacB"
                     /locus_tag="K0W59_000528"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBI3021690.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGILKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFGVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(38315..39487)
                     /gene="cgtA"
                     /locus_tag="K0W59_000529"
     CDS             complement(38315..39487)
                     /gene="cgtA"
                     /locus_tag="K0W59_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HBI3021691.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(39503..40468)
                     /locus_tag="K0W59_000530"
     CDS             complement(39503..40468)
                     /locus_tag="K0W59_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBI3021692.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLSPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(40595..40852)
                     /gene="rpmA"
                     /locus_tag="K0W59_000531"
     CDS             complement(40595..40852)
                     /gene="rpmA"
                     /locus_tag="K0W59_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBI3021693.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(40873..41184)
                     /gene="rplU"
                     /locus_tag="K0W59_000532"
     CDS             complement(40873..41184)
                     /gene="rplU"
                     /locus_tag="K0W59_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBI3021694.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            41443..42414
                     /gene="ispB"
                     /locus_tag="K0W59_000533"
     CDS             41443..42414
                     /gene="ispB"
                     /locus_tag="K0W59_000533"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HBI3021695.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            42642..42920
                     /gene="sfsB"
                     /locus_tag="K0W59_000534"
     CDS             42642..42920
                     /gene="sfsB"
                     /locus_tag="K0W59_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HBI3021696.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPRTHEFIDRTQLMRSYTKPKK"
     gene            complement(42968..44227)
                     /gene="murA"
                     /locus_tag="K0W59_000535"
     CDS             complement(42968..44227)
                     /gene="murA"
                     /locus_tag="K0W59_000535"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBI3021697.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(44282..44536)
                     /gene="ibaG"
                     /locus_tag="K0W59_000536"
     CDS             complement(44282..44536)
                     /gene="ibaG"
                     /locus_tag="K0W59_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HBI3021698.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(44696..44989)
                     /gene="mlaB"
                     /locus_tag="K0W59_000537"
     CDS             complement(44696..44989)
                     /gene="mlaB"
                     /locus_tag="K0W59_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HBI3021699.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(44989..45624)
                     /gene="mlaC"
                     /locus_tag="K0W59_000538"
     CDS             complement(44989..45624)
                     /gene="mlaC"
                     /locus_tag="K0W59_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HBI3021700.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(45643..46194)
                     /gene="mlaD"
                     /locus_tag="K0W59_000539"
     CDS             complement(45643..46194)
                     /gene="mlaD"
                     /locus_tag="K0W59_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HBI3021701.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(46199..46981)
                     /gene="mlaE"
                     /locus_tag="K0W59_000540"
     CDS             complement(46199..46981)
                     /gene="mlaE"
                     /locus_tag="K0W59_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HBI3021702.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHVPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(46989..47798)
                     /gene="mlaF"
                     /locus_tag="K0W59_000541"
     CDS             complement(46989..47798)
                     /gene="mlaF"
                     /locus_tag="K0W59_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HBI3021703.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            48008..48973
                     /locus_tag="K0W59_000542"
     CDS             48008..48973
                     /locus_tag="K0W59_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBI3021704.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLLLGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIIWLAMLYWLSPILVE"
     gene            48987..49973
                     /gene="kdsD"
                     /locus_tag="K0W59_000543"
     CDS             48987..49973
                     /gene="kdsD"
                     /locus_tag="K0W59_000543"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HBI3021705.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            49994..50560
                     /gene="kdsC"
                     /locus_tag="K0W59_000544"
     CDS             49994..50560
                     /gene="kdsC"
                     /locus_tag="K0W59_000544"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HBI3021706.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDCPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            50557..51132
                     /gene="lptC"
                     /locus_tag="K0W59_000545"
     CDS             50557..51132
                     /gene="lptC"
                     /locus_tag="K0W59_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HBI3021707.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            51101..51658
                     /gene="lptA"
                     /locus_tag="K0W59_000546"
     CDS             51101..51658
                     /gene="lptA"
                     /locus_tag="K0W59_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HBI3021708.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            51665..52390
                     /gene="lptB"
                     /locus_tag="K0W59_000547"
     CDS             51665..52390
                     /gene="lptB"
                     /locus_tag="K0W59_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HBI3021709.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            52438..53871
                     /gene="rpoN"
                     /locus_tag="K0W59_000548"
     CDS             52438..53871
                     /gene="rpoN"
                     /locus_tag="K0W59_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBI3021710.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSRLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            53894..54181
                     /gene="hpf"
                     /locus_tag="K0W59_000549"
     CDS             53894..54181
                     /gene="hpf"
                     /locus_tag="K0W59_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HBI3021711.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            54299..54790
                     /gene="ptsN"
                     /locus_tag="K0W59_000550"
     CDS             54299..54790
                     /gene="ptsN"
                     /locus_tag="K0W59_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HBI3021712.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            54836..55690
                     /gene="rapZ"
                     /locus_tag="K0W59_000551"
     CDS             54836..55690
                     /gene="rapZ"
                     /locus_tag="K0W59_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBI3021713.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            55687..55959
                     /gene="npr"
                     /locus_tag="K0W59_000552"
     CDS             55687..55959
                     /gene="npr"
                     /locus_tag="K0W59_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HBI3021714.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            56172..56804
                     /gene="yrbL"
                     /locus_tag="K0W59_000553"
     CDS             56172..56804
                     /gene="yrbL"
                     /locus_tag="K0W59_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HBI3021715.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(56801..57529)
                     /gene="mtgA"
                     /locus_tag="K0W59_000554"
     CDS             complement(56801..57529)
                     /gene="mtgA"
                     /locus_tag="K0W59_000554"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HBI3021716.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSTPDGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(57526..58179)
                     /gene="elbB"
                     /locus_tag="K0W59_000555"
     CDS             complement(57526..58179)
                     /gene="elbB"
                     /locus_tag="K0W59_000555"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HBI3021717.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(58409..60745)
                     /gene="arcB"
                     /locus_tag="K0W59_000556"
     CDS             complement(58409..60745)
                     /gene="arcB"
                     /locus_tag="K0W59_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HBI3021718.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSHRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(60841..61770)
                     /locus_tag="K0W59_000557"
     CDS             complement(60841..61770)
                     /locus_tag="K0W59_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBI3021719.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            62445..66905
                     /gene="gltB"
                     /locus_tag="K0W59_000558"
     CDS             62445..66905
                     /gene="gltB"
                     /locus_tag="K0W59_000558"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HBI3021720.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIHPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            66918..68336
                     /gene="gltD"
                     /locus_tag="K0W59_000559"
     CDS             66918..68336
                     /gene="gltD"
                     /locus_tag="K0W59_000559"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HBI3021721.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            68520..68999
                     /locus_tag="K0W59_000560"
     CDS             68520..68999
                     /locus_tag="K0W59_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HBI3021722.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            69074..69289
                     /locus_tag="K0W59_000561"
                     /pseudo
     CDS             69074..69289
                     /locus_tag="K0W59_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT        16935 a        18808 c        17223 g        16323 t
ORIGIN      
        1 tcggtattcg tattgaaaat ctcggcggcg gagttgcaaa tattcctttt cagaacggta
       61 tccttcccgt tgatccttcc gggcatggca ccgtcaatat gcgcgcctgg ccagttaatc
      121 tggtcggtgg tgagctggaa acaggaaaat ttcagggcac agccaccatt accgtcatgg
      181 tgcggtaaaa gaagacgttg cgttttgatt aacgcaacgc ttcggcactg ttacccctgc
      241 tgctccagcg catacttata cagcgcattt ttcttcacgc catgaatttc tgcggccagc
      301 gccgccgctt ttttcagcgg cagttctgcc tgtagcagcg ccagcgtgcg cagggcatcg
      361 gcgggtaagt cttcttcctg tgctttatga ccttcgacaa tcagcaccat ttcgcctttg
      421 cgacggtttt catcttcctt tacccacgcc agcagctcgc caacgggcgc gccgtgaatg
      481 gtttcccagg ttttggtcag ctcacgcgcc agaaccacgt agcgggattc gcctaatacc
      541 gcaacgatat cttccaggct atctaacaga cggtgggtag attcataaaa aattagcgtg
      601 cgcggctccg cttcaatggc ttttagcgca tcacggcggc cttttgattt ggcaggtaaa
      661 aagccttcgt aacagaaacg gtcagatggt aaacccgcgg cgcttaacgc agtgatagca
      721 gcacacggtc cgggtagcgg caccacacga atccccgctt cacggcaggt acgcaccaga
      781 tggtagccag gatcgttaat tagcggcgtt ccggcatcgg aaaccagcgc aatgttttgc
      841 ccctcttgca gcttcgccag cagcgtttcg gctttttgtt gttcgttatg gtcgtgcagc
      901 gcaaacaacc gggcattaat cccaaaatgt tgcagcaata aacctgtgtg acgagtatcc
      961 tcggcggcaa tcagatcaac ggcctgtaat acctctaacg tacgctgggt gatatccgcc
     1021 agattgccga ttggcgtcgg tacaatgtaa agctggccct gagaattatc cgccgattgg
     1081 tgttgtttca ttgtgtcgtc cgtattgccg atttaatatt gagcattgcg taaaaaaaat
     1141 atcactggat acattatggt accctcaaca ttttctcgtt tgaaagccgc gcgttgtctg
     1201 cctgttgttc tggcagccct gattttcgcc ggttgtggca cccatactcc cgatcagtcc
     1261 actgcttata tgcagggcac ggcgcaggct gattctgcct tttatcttca gcagatgcag
     1321 caaagctctg atgataccag gatcaactgg caattactcg ccattcgtgc actggtgaaa
     1381 gaaggtaaaa ccgggcaggc ggttgagttg tttaaccaac taccgcaaga actgaacgat
     1441 tctcagcgtc gcgagaaaac actgctggcg gcagagatta aactggcgca gaaagatttt
     1501 gctggcgcgc aaaacttgct ggcgaaaatc acacctgccg atttagaaca aaaccagcaa
     1561 gcgcgttact ggcaggcaaa aatcgatgcc agccaggggc gtccttccat tgatttactg
     1621 cgcgcgttaa ttgctcagga accgctgctt ggcgcgaaag aaaaacagca gaatattgat
     1681 gccacctggc aggcgctctc ctccatgact caggaacagg cgaatacgct ggtgatcaac
     1741 gccgacgaaa atattctgca aggctggctg gatctgcagc gcgtctggtt tgataaccgt
     1801 aacgatcccg acatgatgaa agccgggatc gccgactggc agaaacgtta tccgaacaat
     1861 ccgggcgcga aaatgctgcc aacgcagttg gttaacgtaa aagcgtttaa accagcctcg
     1921 accaacaaaa tcgccctgct gttgccgctg aatggccagg cagcggtatt tggtcgcact
     1981 attcagcaag gctttgaagc cgcgaaaaat atcggcactc agccagtggc ggctcaggta
     2041 gctgccgcac ctgccgcaga cgtagcagaa caacctcagc cgcaaactgc ggatggcgtt
     2101 gccagcccgg cacaagcctc ggttagcgat ctgaccggtg atcagcctgc ggcccagccg
     2161 gtgcctgtaa gcgccccggc gacaagcacc gcagcggtaa gcgcacccgc aaatccatcc
     2221 gcagagctga aaatctacga tacctcatca caaccactta gccagatctt aagccaggtt
     2281 cagcaggatg gcgcgagtat tgtggttggt ccgttgctga aaaataacgt tgaagagttg
     2341 ctgaagagca acaccccgct gagcgtactg gcactgaacc agccggagaa tatcgaaaac
     2401 cgcgtcaata tttgttactt cgcgctttca ccggaagacg aagcgcgcga tgcagcgcgt
     2461 catattcgtg accagggtaa acaagcgccg ctggtgctga tcccacgcag tgcattgggc
     2521 gatcgcgtag ccaatgcgtt tgcgcaagag tggcagaaac tgggcggcgg caccgttctg
     2581 caacaaaaat ttggttccac cagcgaatta cgcgcgggtg ttaacggcgg ttctggtatt
     2641 gctttaacgg gtagcccgat tactcccaga gcgacaaccg actccggcat gacgaccaac
     2701 aatccaacgc tgcaaaccac gccaaccgat gaccagttca ccaataatgg cggtcgtgtc
     2761 gatgcggtct acattgtggc aacgccgggt gaaatcgctt ttatcaaacc gatgatcgcc
     2821 atgcgtaacg gtagccagag cggtgcaacg ctgtacgcca gttcccgcag cgcacaaggc
     2881 accgcaggcc cggatttccg actggagatg gaaggcttgc agtacagcga aatcccgatg
     2941 ctggcaggcg gtaatctgcc gttaatgcag caggcgctca gcgcggtgaa caacgattat
     3001 tcgctggctc gcatgtatgc gatgggcgtt gatgcctggt cgttggcaaa tcatttctca
     3061 caaatgcgcc aggttcaggg ttttgaaatc aacggtaata ccggaagtct gacggctaac
     3121 ccggattgcg tgattaacag gaagttatca tggctacagt accaacaagg tcaggtagtc
     3181 cccgccagtt aaccaccaaa cagaccggcg atgcgtggga agcacaagcg cgtcgctggc
     3241 tggaaggcaa aggactgcgg tttatcgccg ctaacgtgaa cgagcgtggc ggcgagatcg
     3301 atctgataat gcgtgaaggc ctgaccaccg tttttgtcga ggtacgctat cgccgctctg
     3361 cgctttatgg cggcgcggca gccagtgtga cccgcagcaa acaacacaaa ttattacaga
     3421 ctgcccgctt gtggctcgcg cgtcataatg ggagttttga tactgtggat tgccggttcg
     3481 atgtggtagc cttcaccggg aatgaggttg agtggattaa ggatgccttt aatgaccact
     3541 cataattaag gtttaaggat tagcgtgcaa gaaagaatta aagcttgctt cactgaaagc
     3601 attcaaactc aaattgcggc ggcagaggcg cttccggatg ccatctcccg tgcagccatg
     3661 acgctggttc agtctctgct caatggcaac aaaatcctct gttgtggtaa tggaacttcc
     3721 gctgccaatg cacagcattt tgctgccagc atgatcaacc gtttcgaaac ggagcggccc
     3781 agcttacctg ccattgcact aaatactgat aatgttgtct taacggcgat tgccaacgat
     3841 cgcttacatg atgaagtgta tgcaaaacag gtgcgggcgc tgggtcatgc gggagatgta
     3901 ttgttagcca tttccacccg tggcaacagc cgcgatattg ttaaagcagt tgaagccgcc
     3961 gttacgcgtg atatgaccat tgtggcattg accggctatg acggcggcga acttgcaggt
     4021 ttgttagggc cacaggatgt ggagatccgc attccttcgc atcgtagtgc tcgcattcag
     4081 gaaatgcata tgctgacggt aaattgcctg tgcgatctga tcgataacac gcttttccct
     4141 caccaggatg attaaggaga atacatgaag gcattatcgc caatcgcagt ccttatttcc
     4201 gcgctgctgt tgcaaggttg tgttgccgct gccgtagtgg gtaccgctgc tgtgggtacc
     4261 aaagccgcaa ctgacccacg cagtgtcggc acccaggtgg acgatggtac cctggaagtg
     4321 cgcgtgaaca gcgcattgtc gaaagacgaa cagattaaga aagaaacccg tattaatgtc
     4381 acggcctatc agggcaaagt gctgctggtt gggcagtcac caaatgctga actttcggct
     4441 cgcgccaaac agattgctat gggcgtagac ggtgccaacg aagtgtataa cgagattcgt
     4501 cagggccagc cgattggtct gggcgaagca tctaacgata cgtggatcac caccaaagtg
     4561 cgttcgcagc tcttaaccag cgacctggtg aaatcgtcca acgtgaaagt gaccaccgaa
     4621 aacggtgaag tgttcctgat ggggctggtg actgaacgtg aagcgaaagc ggcggcagat
     4681 attgccagcc gggtgagcgg cgtaaagcgg gtaaccacag cgtttacgtt tattaaatag
     4741 cagccccttg taatgcctga tgcgacgctt gccgcgtctt atcaggccta caacgatgca
     4801 aaccgtaggt cggataaggc gtttacgccg catccgacat gttttccctc aaatcagaac
     4861 aacagcgcca gcccgccgac aatcacacca gacactgcca ccatcgcccc tgtcagccat
     4921 aaggctttcg ccgggaacgc tttgcgcagc ataatcagtg acggcaaact cactgccggg
     4981 agcgtcatca acaatgccag cgccggagcg gttcccatac ctgccagcat catcgtttgt
     5041 acaatcggaa tttctgctgc cgtgggaatg acaaacaagc atcctgctac cgccatcgcc
     5101 accacccaca tcaggctgtt atcgacagca ccatcggcat gggggaataa ccagacgcgt
     5161 gcggcaccca acaccagtac tgcaaggatg taaaccggga tcgtactcca gaaaagtgtc
     5221 catagcgccc tgccccagcg gctaaaaaaa ccgctctgtg cttccggtat gtcaatttcg
     5281 accggtgcct gcgtttgcgg tgtttcacgc acccattttt gcaccagcgt cgcaatcagc
     5341 aacaccatca ccagcccggc caccagacga atcgccgcaa aaccccagcc gaggacaaac
     5401 cccataaaca ccagcgtcgc cgggtttaac accggattgc ccatccagaa tgccagcgca
     5461 ccgcccatcg atacctgttg gcgacgcatt cccgccgcga ccggagccgc acagcaggta
     5521 cacatcatgc ccggcagcga aaacagcgtt cccagcagcg tgccgcgaaa gcgcgattgc
     5581 ccaagcgtac gcaacaacca gtcacgcggg atcagcacct gaatcaacga accgaggatc
     5641 acccccagca ccgccgcttt ccatactgcg aggaaataga tcatcgcgta atccaacgcc
     5701 gcttgccacg gattagcatc cgcctgcgca aggatagatt taccgatact gtgggtttcg
     5761 gctgcagtaa acgctttgcc gtagtaaggt tgccatttca cataccagag accggcaatg
     5821 acaacgagaa agaaaagcgc gggtttccac cactgaatgg gcgttgccgc ctgagatgaa
     5881 gactgaccag tcatagcatt ccccaggaat aagttatgat aattgagcgc gtgaatatta
     5941 cgctcactat caattcttgg gaataattat tcagctcttt tgcgtaattc tgaagagctt
     6001 aaaatcgtca cgccttcatg ctcaggccgc atcgcctctg ccagcatgac ccgcgccacg
     6061 tctctcgcat caatggattt ccagttacct ggtaacaaac ggaacagcgg cgcaaaaagc
     6121 gtttcgttca tccgctgttt gctacgatcg cccagtaaca tcgacgggcg agcaatggtc
     6181 aatttcggcc agttctgggc aattaatgct tcttccatct cccctttgac gcggttatag
     6241 aaaaacggcg agtgggcatt ggcacccata gcactgacca ccagcatatg ctgcgcgccc
     6301 agtcgccgcc cggttaatgc ggtatccact accagcgtgt aatcggcatg aataaacgcc
     6361 tctttgctcc ccgcttctcg ccgcgtggtg ccgagacaac aaaacacaat gtcgatgggt
     6421 tcggtgacct gcgccaacgc gtcactcagt tgcggatcat ggggattaaa caccccgggc
     6481 atatcgccca acggacgtcg cgtcggcgca gcaatggcgt taactttcgg ttcattaatc
     6541 aacatccgca gcaggtgacc gcccaccagc cccgttgcgc ctgtaatcag tacctgactc
     6601 atcttcgctc ctttacagaa ttgtctgcct tgcgctcccc ggctcatgca ccagtcttaa
     6661 tagaccatga ggtaattatc cccgattgtg gaaaattcgc ctcatccaat gcaacaacgc
     6721 ggaggaagca tgagtaagaa aattgccgtt ttaatcactg atgaatttga agattcagaa
     6781 tttacttcac ccgcagacga gttccgtaaa gccggacacg aagtgattac tattgaaaaa
     6841 caagcgggta aaacggtgaa aggcaaaaaa ggagaagcca gcgtgaccat cgataaatcc
     6901 atcgatgaag tgacgtcagc ggagtttgat gccctgttgc taccgggtgg tcattcaccg
     6961 gattatctgc gtggcgacaa ccgttttgtc acctttaccc gtgattttgt gaatagcggc
     7021 aaacctgttt tcgccatctg tcacggtccg cagctgctga tcagcgccga tgtgattcgg
     7081 gggcgcaaac tgaccgcagt taaaccgatc attattgatg ttaaaaatgc gggcgtggaa
     7141 ttttacgatc aggaagtcgt ggttgataaa gatcagctgg ttaccagccg gacaccggac
     7201 gatctgccag cgtttaaccg cgaggcgtta cgcctgctcg gtgcctgagt cgcgtagcca
     7261 aatcattttt ttaccaaagc ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat
     7321 ttcccacacc ggggccgaca gcattctggc aaccggaaag cgacgattgt acgccttgcg
     7381 cgcgaggtcg ctttcctcac cttccagcct gcggatctca cctttaaact gcacaccgcg
     7441 aattaacgct accgttttcg gctgaccgtt taccgttccg gcaaccgcag cctgcggccc
     7501 actcatctgc gcgtggcgcg ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc
     7561 atcaaaaaga taaaaggcat tagcgcacca gagttcccct tcctgctgca cacaccaggt
     7621 gacaacatgt tgttttgcca gccaacggct gatggcgatg agtgtttcca ttgctgttct
     7681 cttttatact gtgggcctga ccttaacatg cgatgcagac gatgaccccc tggtttcttt
     7741 acttgatccg taccgccgac aataagcttt ataccgggat caccacggat gtcgaacgcc
     7801 gctatcagca gcaccaaagc ggcaaagggg cgaaagcact gcgcgggaaa ggagaactga
     7861 cgctggcgtt ttctgcgcca gtgggcgacc gttcgctggc actacgggcg gaatatcgcg
     7921 ttaagcaact gaccaaacgg caaaaagagc gtctggtagc ggaaggcgca ggggttgcag
     7981 aactgttgag cagcctgcaa accccggaga ttaaaagcga ttgaaatgct cgtgatactc
     8041 aaccagaccg gttacgccat tcagcgcgtc atccgctaaa cgatgtacct ggaaggcgct
     8101 ttcagtgccc ggccagcggc aacgcagatc gtgatgcgcc gccagttcaa agccgaaacg
     8161 actgtacagc gccggatcgc ccagcgtcac cactgcggca tagccgaact cattaagcga
     8221 atcgagtcct tcatagacca gttggcgtgc cagcccctgt ccacggtatt tttcatcaac
     8281 cgccaacggt gccatgccga cccattgcag gtcttcgccc tgcacatcaa ccggactaaa
     8341 tgccacatag ccaatgacct gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa
     8401 gccatcttca cgcagatcgt gaaccagctt cgcttccgca tcactttcga atgagcgacg
     8461 cagcagggca tcaatacccg gcgcatcaat gggaatttct actcgaatta gcatggttca
     8521 cctaccgatg tctgtttggt ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc
     8581 agttgcagca gcatcatgcg taaggctttc ggcatttgct ccagttcaat ggcgtccatc
     8641 aggtttttca catacacccc cagctccgta tcgccttcaa tcaccagccg acgctggaag
     8701 aagagcgtat ccggatcttg tttacgcgcc gcaatcatca gcagatcgct ggcgtcggca
     8761 ctaaaactca catcagcttg cgcgttctgg ctgaccacca gtttgccatt caccaccgag
     8821 gtaaaccatt gcaggtcaat atcacgcaca tgaatactta accagcggcc ttcaagaaac
     8881 tccagctcgc catcatccag cgcctgacgg aattgccagc ttaagacctg ctcaagaacc
     8941 tggcgtttta gcgcaaatgg cgtcagtttt accggtacac tcaacagaga tggccccaaa
     9001 tgcacaatac gggaacgcag tttatccaac acgagtttta ctccctgttt caacaatcat
     9061 cctattttgc catatcagaa aaataacata gcggtataaa tcaacaattc catatgaaat
     9121 tgctgctacc accaatacaa ctttaactgc cttaaatcaa aaattgtcgc agcaaggtta
     9181 actaaaatcc cagttcgtta acatttttgc gttttgatag cgcaaccttc aggaaaaatt
     9241 atggagctgc tctgccctgc cggaaatctc ccggcgctta aggcggccat cgaaaacggc
     9301 gcagatgctg tttatatcgg gctaaaagat gataccaatg cccgtcactt cgccggcctt
     9361 aactttaccg agaaaaaatt gcaggaagcg gtgagttttg tccatcaaca tcgccgcaaa
     9421 cttcacatct cgattaacac ttttgcgcat ccggacggtt acgcccgttg gcagcgtgcc
     9481 gtggatatgg cggcgcagct gggtgccgac gcgctgatcc tcgccgacct cgccatgctg
     9541 gagtacgccg ccgagcgtta cccgcatatt gaacgtcatg tgtcagtgca ggcttcggcg
     9601 accaatgaag aggcgattaa cttttatcat cgccattttg acgttgcccg cgtggtgctg
     9661 ccgcgcgtgt tgtcgattca tcaggtgaaa cagctggcac gggttactcc tgtcccgctg
     9721 gaagtgtttg ctttcggcag cctgtgcatt atgtcggaag gccgttgcta tctgtcgtcg
     9781 tatctgacgg gtgagtcgcc caacactgtg ggtgcgtgtt ctccggcccg tttcgtgcgc
     9841 tggcaacaaa cgccgcaggg gctggaatcc cgcctgaacg aagtgctgat cgaccgttat
     9901 caggacggcg aaaacgcagg ttatccgacg ctgtgtaaag ggcgttatct ggtggacggc
     9961 gagcgctatc acgcgctgga agaaccaacc agtctcaata ccctggaact gctgccggag
    10021 ttaatggctg cgaatattgc ttcggtgaaa attgaaggcc gccagcgtag cccggcgtat
    10081 gtcagccagg tggcgaaagt ctggcgtcag gctatcgacc gttgtaaggc cgatccgcaa
    10141 aacttcgtac cgcaaagcgc gtggatggag acgctcgggt cgatgtccga aggcacgcaa
    10201 accacccttg gcgcgtatca ccgtaaatgg cagtgagaag agcaatgaaa tattccttag
    10261 ggccagtgct gtggtactgg ccaaaagaga cgctggaaga tttttatcag caagccgccg
    10321 ccagcagcgc cgacgtgatt tatcttggtg aagcggtatg cagcaagcgc cgggcaacca
    10381 aagttggcga ctgtctggag atggcaaaat cgctcgccgg aagtggcaag cagattgtgc
    10441 tctccacgct ggcgctggtg caggcatcct ctgaactggg cgaactgaaa cgctatgttg
    10501 agaacggtga gtttctgatt gaagccagcg atctcggcgt ggtgaatatg tgcgctgaac
    10561 gcaaactgcc gttcgtcgcc gggcacgcgc tgaactgcta caacgcggtg acgctgaaaa
    10621 tattgctcaa acagggcatg atgcgctggt gtatgccggt ggagctttcc cgcgactggc
    10681 tggtgaatct gcttaatcag tgcgatgagc tgggcattcg caaccagttt gaagtggaag
    10741 tcctgagcta cggtcatcta ccgctggcct actccgcccg ctgctttacc gcgcgttcgg
    10801 aagaccgccc gaaagatgag tgtgaaacct gctgcattaa gtatccgaac gggcgcaacg
    10861 tgctgtcgca ggaaaaccaa caagtgtttg tactcaatgg cattcagacc atgagcggct
    10921 acgtttacaa cctcggtaac gagctggcat ccatgcaggg attggttgat gtagtccgcc
    10981 tgtcaccgca gggcactgac actttcgcga tgctcgacgc cttccgcgct aatgaaaatg
    11041 gcgctgtgcc actgccgttg acggcaaaca gcgactgtaa cggctactgg cggcggctgg
    11101 caggactgga gctgcaagcc taagtaaata gctcactttg ttaacaactt taactattct
    11161 ttaatgcagt attaaagatt aatcggtaac aaagtgagct gttatgactg ataaaaccat
    11221 tgcgttttcg ctactcgatc tggcccccat acccgaaggc tcttcagcgc gggaagcgtt
    11281 ctcccactct ctcgatctcg cccgtctggc tgaaaagcgc ggctatcatc gctactggct
    11341 ggcagaacac cacaatatga ccggcattgc cagtgctgca acatcggtat tgattggcta
    11401 tctggcggcg aataccacca cgctgcatct ggggtctggc ggcgtgatgt tgcctaacca
    11461 ctcaccgttg gtcattgccg aacagttcgg cacactcaat acgctctatc cggggcgaat
    11521 cgatttgggg ctgggtcgtg ctccgggtag tgaccaacgg acaatgatgg cgctacgtcg
    11581 tcatatgagc ggcgatattg ataatttccc ccgcgatgtc gcggagctgg tggactggtt
    11641 tgacgcccgc gatcccaatc cgcatgtgcg cccggtacca ggctatggcg agaaaatccc
    11701 cgtgtggttg ttaggctcca gcctttacag tgcacaactg gcggcgcagc ttggtctgcc
    11761 atttgcgttt gcctcacact tcgcgccgga tatgttgttc caggcgctgc atctttatcg
    11821 cagtaacttc aaaccgtcgg ctcggctgga aaaaccatac gcgatggtgt gcatcaatat
    11881 tatcgccgcc gacagcaacc gcgacgctga atttctgttt acctcaatac agcaagcctt
    11941 tgtgaagctg cgccgtggcg aaatcaggca actgccgccg ccgattcaaa atatggatca
    12001 gttctggtca ccgtctgagc agtatgacgt gcagcaggcg ctgagtatgt cgttggtagg
    12061 cgataaagcg aaagtgcgtc atggtttgca gtcgatcctg cgcgaaaccg acgctgatga
    12121 gattatggtt aacgggcaga ttttcgacca ccaggcgcgc ctgcattcgt ttgagctggc
    12181 gatggatgtt aaggaagagt tgttgggata gtgtgtctta acgcgggaag ccttatccga
    12241 gctggcaacg ctgtcctacg taggcctgat aagcgaagcg catcaggcat tgtgtaggca
    12301 gcagaaatgt cggataaggc accgctgatt actgatacac cggcagtaaa ttaaagctcg
    12361 ataaaatatg caccagtgcg ttgccgacgc caaacaccag aatcagcgca atcatcggct
    12421 tgccacccca gacgcggaat ttcgggctgc caaagcgttt acgcgatgcg cgggccagca
    12481 gtgccggaac aattgccgcc cagatggtag ccgctaaacc agcataacca atggcataca
    12541 ggaatccgtt cgggaacagc agaccaccca caacaggcgg ggcaaaggtc agcaatgccg
    12601 ttttcaagcg gcccacagcc gagtcgtcga aaccaaacag atctgccaga tagtcaaaca
    12661 aacccagcgt tacgccaagg aacgaactcg ctaccgcaaa gtttgagaac acgaccagca
    12721 gaaggtccag gctacggctg ttcagcacgc cgcttaatgc ttgtaccagc acatcaatat
    12781 taccgccctt ctcggcgatg ccgataaact ccggacgagg gatgttgccc attgtcgcca
    12841 gcaaccagat ggtatacagc gccagtgcca tcagcgtacc gtacaccagg catttcacga
    12901 tggttttcgg atctttgccg taatacttca tcaggcttgg cacgttaccg tgataaccaa
    12961 acgacgccaa gcagaacggc agggtcatca gcaaatacgg cgcataagat gcattgcttt
    13021 cggcaacgtt gaacaatgtc gcaggctgca catgccccag caggctaccg aaggtgagga
    13081 agaaggtaat gactttcgcc cccagcacaa tcgccgtcat gcggctgacg gctttagtac
    13141 tcaaccacac cacaaacgct accagcagtg caaaaccaaa acccgccgcc cgtgccggga
    13201 cgtttagcga catctctgcg aaggtgtgat gcagaatcga accactggca gaaatatagg
    13261 cataggtcag gatatagagc acaaaggcaa tggaaatgcc gttgaccacg ttccagcctt
    13321 tgcccagcaa atctttggtg atggtgtcaa aactcgaacc gattcggtaa ttcaggttag
    13381 cttccagaat catcaagccg gaatgcagca tacagaacca ggtaaagatc agcgctgcca
    13441 ttgaccagaa aaaccacgcc ccggacatga ccactggcag agaaaacatc cctgcgccaa
    13501 taatggtgcc gccgataatc accacgccgc caagcagcga cggtgacgtt tgggtggtgg
    13561 ttagtgttgc catgagggct tctctccagt gaaaaatagt gcgactgcgt tgttatgcat
    13621 tgcactgtac cagtacatga gtacaaaaga cagaaaaaaa gccccgatgg ttaaaatcgg
    13681 ggctgtatat attattttac agattgtgtt cgctgttcag cgatgattac gcatcaccac
    13741 cgaaacgacg acgaccggta gagtcatcac gacgcggtgc gcggccttca cgacgttcgc
    13801 cgctaaaacg acgaccatca ccacgaccac cttcacggcg ttcaccgctg aagttacgac
    13861 cgccttcacg acgctcgcca ccgaaaccac gaccaccgcc acgaagctca ccgccagtat
    13921 gcggctgtgc atcgcccagt aactgcatgt tcatcggctt gttgagaatg cgagtgcgcg
    13981 taaagtgttg cagcacttca cccggcatac ctttcggcag ttcgatggtg gagtgagaag
    14041 caaacagctt gatgttaccg atgtaacggc tgctgatgtc gccttcgtta gcaatcgcgc
    14101 caacgatatg acgaacttca acaccatcat cgcggcccac ttcaatgcgg tacagctgca
    14161 tatcgccaac atcacgacgt tcacgacgcg gacgatcttc acggtcgcca cgcgggccac
    14221 ggtcgttacg atcgcgcgga ccacggtcat cacggtcacg gaattcacgt ttcggacgca
    14281 tcggcgcatc tggcggaaca atcagagtac gttcaccctg tgccattttc agcagtgccg
    14341 cagccagagt ttcgagatcc agctcttcac cttctgcagt cggctgaatt ttgctcagca
    14401 gtgcgcggta ttgatccaga tcgctgcttt ccagctgctg ctgtacttta gcggcgaatt
    14461 tttccagacg gcgtttgccc agcagttctg cgttcggcag ttctacttcc ggaatagtca
    14521 gcttcatagt acgttcaatg ttgcgcagca gacgacgctc gcggttctca acgaacagca
    14581 gcgcgcggcc agcacgaccc gcacgaccgg tacgaccgat acggtgaacg taagactcag
    14641 aatccatcgg gatatcgtag ttaaccacca ggctgatacg ctcaacgtcc aggccacggg
    14701 ccgcaacgtc ggtcgcaatc aggatgtcca gacgaccgtc tttaaggcgt tccagtgtct
    14761 gctcacgcag cgcctggttc atgtcaccgt tcagcgcggc gctattgtag ccgttacgct
    14821 caagagcttc agccacttcc agagtcgcgt ttttggtacg aacgaagata atcgccgcat
    14881 caaaatcttc cgcttccagg aaacgtacca gtgcttcgtt tttacgcata ccccacacag
    14941 tccagtagct ctggctgatg tcaggacggg tagtcacgct ggactgaata cgcacttcct
    15001 gcggctcttt cataaagcgg cgggtaatgc gacgaatcgc ttccggcatg gttgcagaga
    15061 acagagcggt ctgatgacct tccgggatct gcgccataat ggtttcaacg tcttcgatga
    15121 agcccatgcg cagcatttcg tcagcttcat ccagaaccag accgctcagt ttagagaggt
    15181 ccagagtgcc acgtttcagg tggtccagca gacggcccgg agtaccgaca acgatctgcg
    15241 gcccctgacg cagggcgcgt aattgcacgt cataacgctg gccgccgtac agggcaacca
    15301 catttacgcc gcgcatgtgt ttagagaaat ccgtcattgc ttcagcaacc tgtaccgcca
    15361 gttcgcgggt cggtgccagc accagaatct gtggtgcttt cagctcagga tcaagattct
    15421 gcagcagagg taaagagaat gctgcagttt ttccgctccc cgtctgggcc atacccagaa
    15481 cgtcgcggcc attcagcaga tgtggaatac actctgcctg aattggagat ggtttttcgt
    15541 aacccagatc gttaagggct tcaaggatag gagccttcag gcccagatct gcaaaagtgg
    15601 tttcgaattc agccatgtag tacgtgtgcc tcaaaattaa tggcggccag tctacataac
    15661 tcatcatgaa attgatcagc aattttcatt gaaaagtgtg aaccggctca aagtaggtgt
    15721 attaacgaac aacaacgccc tcacccgtta aggtgatggc aatcaaaaaa gattacgggc
    15781 tgatgtgtac gtcagctatt gctggtccga ttctgccagg tcatcttggt cctggcccag
    15841 gagcgataat tccaacaatg cgtatcggtg ctcaacaaag ttatgaacgt tgttggcaac
    15901 cgccagtttg aacagtgccg tggcgctgtc caaatccccc agacttaggt agtacttacc
    15961 taaatagaag ttggtttcac tgagatgctc agcgagcgag gtgttatcgg ttgcgtccgc
    16021 cttgagcctt tccattaacg tttgttcgct aatgttgccc aggtagaact cgacaatgtt
    16081 ccatccccac tgttccttat ccgatttttc gaagtgctgt ttcaacactt ctttagcctg
    16141 cttctcatcg agcttctgct cggcgagata aagccacaga ctacggaaag gatcattggg
    16201 atcgtcttga taaaacgcca gcagatcatc ttgcgctaac ttgtcacgac cgccgtaata
    16261 taatgcaatc ccgcgattca agtgcgcgta gttgtaagtt ggatcaagct caagtacaga
    16321 atcaaacgct tcataggcag catcaaaatt gcctgcctgc gttaaatata tgcctaagta
    16381 attgaatact tcaggcatat ccggtcggat tgccagcgct tgcgaaaaat cgttacgcgc
    16441 taatgccctc agaccgagac tatcatacaa cactccgcgc tcatataaaa gctgtgcgcg
    16501 ttcgtcatcg gttaaagccc gactggcaag gatttgttcc atacgtgcca gaatcacttc
    16561 ctgctgtaaa gtcggttgca atggtaccgc gaggacttca cttttacgcc aggaagtatt
    16621 actgcatcct gcaagcgtaa gtgctgtcgc aacgaaacac cagcgcaaaa aaggcttcat
    16681 ttcccactcc cgaagaccac ggttgaatga acgtcctgtt cccggttgct aacaaggcgt
    16741 cctgcccggt taaaagcccc ccgccgcagc ggagggcaaa tggcaacctt actcgccctg
    16801 ttcagcagcc ggagcttccg gtgctgcagc aggttgagac tgctcagtcg cttctttaat
    16861 gctcagacgg atacggccct ggcgatcaac ttccagaact ttcaccggta cttcctgacc
    16921 catctgcagg taatcggtca ctttctcaac gcgtttgtca gcgatttgag agatgtgtac
    16981 cagaccttct ttaccgccgc cgatggcaac aaatgcgcca aagtcaacga tacgggtcac
    17041 tttaccagtg tagacgcggc ccacttcgat ttctgcagtg atctcttcga tacgacgaat
    17101 agcatgtttc gctttctcgc cgtcggtcgc tgcaatcttc acagtaccgt catcttcgat
    17161 ttcgatggtg gtgccagttt cttcggtcag ggcacggata acagagccgc ctttaccgat
    17221 aacgtctttg atcttgtccg ggttgatctt gatggtatgg atacgcggtg cgaactcaga
    17281 gatatcgcca cgcggcgcgt tgatcgcctg ttccattacg cccaggatat gcagacgcgc
    17341 acctttagcc tggttcagcg caacctgcat gatctctttg gtgatacctt caattttgat
    17401 atccatctgc aatgcagaga taccctcgcg ggaacccgca actttgaagt ccatatcgcc
    17461 caggtgatct tcgtcgccca aaatgtcaga cagtacaacg tagttgtcgc cttctttcac
    17521 cagacccatt gcgatacccg caacggcagc tttgatcggc acacctgcgt ccatcagcgc
    17581 cagagacgcg ccgcacacgg aagccataga agaggaaccg ttggattcag tgatttcaga
    17641 cacaacacgt acggtgtacg ggaatttgtc catatccggc atgactgcca gcacgccgcg
    17701 cttcgccaga cgaccgtgac caatttcacg acgcttcgga gaaccgacca tgccggtttc
    17761 gcctacggag tacggaggga agttgtagtg gaacaggaag gtatcggtac gttcgcccat
    17821 cagttcatca agaacctgcg cgtcacgagc agtacccagc gttgcggtaa ccagcgcctg
    17881 cgtttcacca cgggtgaaca gcgcagaacc gtgagtacgc ggcagcacgc cagtacgcac
    17941 atccagacca cggatcatat ctttttcacg accgtcgata cgcggttcgc ctgccagtac
    18001 gcggctacga acaacgtttt tctcgatcgc gtgcagaatt tcacccagtt cgttttcgtc
    18061 cagggtttcg tcttcagcaa gcagcgtcgc gatggtttca gatttgatga catcaacctg
    18121 cgcataacgc tcttgtttgt cggtgatgcg gtaagcatcg ctcaggcgag cttcagccag
    18181 tgcagcaacg cgcgcgttca gcgcttcgtt taccggctcc ggctgccagt cccaacgtgg
    18241 tttaccggct tctttcacca gttcattgat gttctgaata acaacctgct gttgttcatg
    18301 accgaacact actgcgccca gcatctggtc ttcgctcagc agttcagctt cagattcaac
    18361 catcagtaca gcggcttcag taccggcaac aaccagatcc agtttgctct ctttcaactc
    18421 gtcctgagtc gggttcagta cgtactggtc attgatgtaa cctacgcgag cagcaccaat
    18481 cggaccattg aacggaatac cagacagaga cagtgctgcg gaagcaccaa tcatcgcgac
    18541 gatatccggg ttaacttgcg ggttaacaga aaccacggtg gcgataacct gaacttcgtt
    18601 gacgaagcct tccgggaaca gcgggcgaat cgggcggtca atcagacgcg cgatcagggt
    18661 ttcgccttcg cttgggcggc cttcacgacg gaagaagcta cccgggatac gaccagcagc
    18721 gtaggtacgc tcctgatagt taacggtcag tgggaagaag tcctgacctg gtttggcttt
    18781 tttctggcca acaacggtaa caaataccgc ggtgtcatcc atgctaacca taacagcggc
    18841 agtagcctga cgagccatca tgccggtttc cagagtcacg gtgtgttggc cgtactggaa
    18901 tttacgaacg atcggattaa gcaatgtaat atcctttctc tttcttagac agtaccttac
    18961 ggcactggtg ttaatacccg atcttctgcg catcctcgcg actaatgaca accctaaccc
    19021 agctctatgt gggtaaagcc tctcattagc cgcgcgaacc tctgcaacgg aagatcattc
    19081 atagcaacaa tacattagtt tccagtgaat tgctgccgtc agcttgaaaa aaggggccac
    19141 tcaggccccc ttttctgaaa ctcgcaagaa ttagcgacgc agacccaggc gctcgatgag
    19201 ctgggtgtaa cgtgctacgt ctttacgttt caggtagtcg agcagtttac gacgctgaga
    19261 aaccatgcgc agcagaccac gacggctgtg gtgatctttt ttgtgctctg caaagtggcc
    19321 ctgcaggtgg ttgatctgtg cagtcagcag tgctacctga acttcagtag aaccggtgtc
    19381 gtttgcgtca cgaccaaact cagaaacgat tttagctgtt gcttcagtac ttagagacat
    19441 tttaaaactc caaagtatat agaatgaaag gacgccgatc tctaattcag cgatcccagt
    19501 gtacgttacg caaagtgtta aataatttac gcgacgttaa gcggcagtat tctactcgta
    19561 gcgacctgtt atcgcaagac ggttaacatt acgccgggta ttcaaccacc aggcgacgag
    19621 gcgcaacgcg gccttcatcg tcaatttcgc ccataccgat aaatttgccg ttctcacctt
    19681 ctgtaacgcg aaccagtcct tccagcggcg caccggatgt acgaactggg ttaccatttt
    19741 tgaagtaaac agaagacgtt aacggaagat tcaccaccgg gtagtccgaa gctggactgt
    19801 ccattggcat cagtaatgga tcaagtaact ccgcagccgg aatatcctgc tgttcggctt
    19861 gttcgaccag ttcacgcaga tgttccaggg tcacaatccg ttcaaccgga tatttactta
    19921 ccgccagacg acgcaggtaa ataacatgcg cgccacaacc gagtttttca cccaggtcat
    19981 caatgatggt gcggatataa gtgccttttg agcagtgaat ttccagctcc agctcattgc
    20041 cttcgtggcg aataaacagc aattcataaa cggtaatcgg gcgcgcttca cgcggtactt
    20101 caatgccctg acgcgcatat tcgtacagtt ttttaccctg atatttcagt gctgaataca
    20161 tcgaagggat ctgttcgata tcgccacgga aagtatccag cgccgccgcc agttgctctg
    20221 cgctaaaggt taccggacgt tcttcaacga tttgtccgtc ggcatcagaa gtatcggtac
    20281 gctgtccaag acgcgcaatg acccgatagc gtttgtcgga gtccagcaga tactgggaaa
    20341 acttcgtcgc ttccccgagg caaattggca acatgccagt tgccagcggg tccagcgcac
    20401 cggtatgccc ggcacggttg gcgttataaa tccgtttcac tttttgcagc gcatcattgc
    20461 tggacatacc ctgcggttta tccagcaaca aaacgccgtt aatgtcgcga ccgcgacgac
    20521 gaggacgact cattagtcct ccttgctgtc gtccgggtta acacgacgtt cttcgtcatg
    20581 tttgaccacg ctggtcacca ggtttgacat gcgcatccct tcaaccagag agttgtcgta
    20641 gaagaaggtc agttccggca cgatacgcag gcgcatcgct ttccccagca ggctgcggat
    20701 gaaaccagaa gcttcttgca acgctttgat gcccgcttta accgcgtctt catctttgtc
    20761 gttgaggaac gtcacatata ctttggcata cgccaggtcg cgagacattt cgacaccgga
    20821 aacggtggtc atcatgccca ggcgaggatc tttaatttca cgctgcagga tgagagcgat
    20881 ctctttttgc atttcctgcg ctacgcgctg cgggcgacca aattctttcg ccataataaa
    20941 ttctcctgac aaaaaagggg ctgttagccc ctttttaaaa ttaatttcag gtggaagggc
    21001 tgttcacgtt ggcctgataa gacgcgccag cgtcacatca ggcaatccat gccggatgcg
    21061 gcgtaaacgc cttatcccgc atggaaccct aaaaacctta agcgatggta cgttggatct
    21121 cgatgatttc gaatacttcg atcacatcgc cagtgcggac gtcgttgtag ttcttaacgc
    21181 cgataccaca ttccataccg ttacggactt cgttaacgtc atctttgaag cggcgcaggg
    21241 actccagctc gccttcgtag ataaccacgt tgtcacgcag aacgcggatc gggttgtgac
    21301 gtttaaccac accttcggta accatacagc ctgcgatggc accaaatttc ggcgatttga
    21361 acacgtcacg aacttccgcc agaccgataa tctgctgttt cagttccgga gacagcatac
    21421 cgctcatcgc cgctttcact tcgtcaatca ggttatagat gacggagtag taacgcagat
    21481 ccaggctttc cgcttcaatc actttacgtg cagaggcatc agcacgtacg ttgaagccaa
    21541 ccaggatggc gttggacgcc gcagccaggg tggcgtcggt ttcggtgata ccacctacgc
    21601 cagaaccgat gatcttcact ttaacttcgt cagtagacag tttcagcaag gagtcggaga
    21661 tcgcttccac agaaccctgt acgtctgcct tcaggacgat gttcacttcg tgaacttcgc
    21721 cttcggtcat gttggcgaac atgttctcga gtttagattt ctgctgacgc gccagtttaa
    21781 cttcgcggaa tttaccctga cgatacagtg caacttcacg cgctttcttc tcgtcacgta
    21841 caacggtaac ttcatcaccc gcagccggta cgccggacag gccgaggatt tccaccggaa
    21901 tggacggacc cgcttccagc acttcctgac ccagttcgtt acgcatcgca cgaacacgac
    21961 cgtattcgaa gccacacaga acgatatcgc ccttgtgcag agtaccttca cgtaccagaa
    22021 cggtagcaac cggaccacga cctttatcga ggaaggattc gataaccgca ccgctcgcca
    22081 tacctttacg taccgctttc agctccagaa cttccgcctg cagcaggata gcgtccagca
    22141 gttcatcgat accagtaccc gctttcgcag acacatgtac gaactggctt tcaccgcccc
    22201 actcttccgg caggatgccg tactgggaga gttcgttctt aacgcgatcc ggatcggctt
    22261 ctggtttatc gatcttgttc actgcaacga ccaccggaac ctgcgccgct ttcgcgtgct
    22321 ggattgcttc gatcgtctgc ggcattacgc cgtcgtcggc agcaacaacc aggactacga
    22381 tgtccgttgc ctgcgcacca cgagcacgca ttgaagtaaa cgcagcgtgc cccggggtgt
    22441 ccaggaaggt gatcatgccg ttttcagttt caacgtggta tgcaccaatg tgctgagtaa
    22501 tgccgcccgc ttcgccagag gccactttcg ttgaacgaat gtagtccagc agagaggttt
    22561 taccgtggtc aacgtgaccc atgatggtca caaccggcgc gcgcggttca gccgcagcac
    22621 ccgtgtcacg gtcgctcatt accgcctctt ccagctcgtt ttcacgacgc aggataactt
    22681 tatggcccat ctcttcagca accagctgtg cggtttcctg atcgataacc tggttgatgg
    22741 ttgccattgc gcccagtttc atcatcgctt tgatgacctg agagccttta accgccatct
    22801 tgttcgccag ttcgccaacg gtgatagttt cgccgatcac aacgtcacgg ttaacggcct
    22861 gagcaggctt ctggaagcct tgctgcagcg aagaaccttt acgttttccg cctttaccgc
    22921 cacgtactgc tgcgcgtgct tcttcacgat cagcttttga ttcagcgtgt ttgttgcctt
    22981 tcttcggacg cgctgctttc gcgttacgac cacggccacg gccgccttcg acttcacgat
    23041 cgctttcgtc ttctgcctgg cgagcatgtt gagaagtagt gacgtgataa tcgctggaat
    23101 cttcagtcgg ttccgcgtta tcagtccatt tgttttcttc cgccatacga cgtgcttctt
    23161 cagcaacgcg acgtgcttct tcttcgagtt tacgacgcgc ttcttcttca gctttacgct
    23221 tgagctctgc agcttcctgc tcacggcggg ctttttcagc ctgggcgttt ttagtcatat
    23281 cgtcttgttg attgctcact ttgtcttttt ccgcagcttc acgtttcgct tgttcagcag
    23341 cttcacgctt agcttgttct gcggcctcac gttcagcttt ttgttgcgcc tcgcgtttag
    23401 ccgattcttc tgcctcacga cgggcttgct cttccgctgc aaggcgttca gcctcttgcg
    23461 gatcgcgttt cacaaaggtg cgtttcttgc ggacttcgat ttgtaccgat ttgctttttc
    23521 caccggtacc aggaatgtta agggtgctgc gtgttttacg ttgcagcgtc aatttgtccg
    23581 ggcctgaatt tttctgattc aggtggtcaa tcaaagtctg tttctcttgt gcagacacag
    23641 agtcgtcagc agacttccgg atacctgcat cagcaaattg ctgtaccagg cgttccacgg
    23701 aggtctgtcg ctctgcggcc agcgttttaa tcgttacatc tgtcatgctg ttccttcctg
    23761 ctacagttta ttacgcttcg tcaccgaacc agcaaatatt acgggcagcc ataatcagtg
    23821 ctccggcttt ttcgtcggtc aacccttcga tatcagccag atcatcaatg ccctgttcgg
    23881 cgagatcttc cagcgtacaa acgccacggg cggccagttt gaatgccaaa tcacgatcta
    23941 ccccttcaag gttcagcaga tcgtcagccg gtttgttatc accgaggctt tcttcctggg
    24001 cctgtgcaat ggtggccagt gcatttttag cacgctcgcg cagtgcttca acggtcggct
    24061 catcaaggcc ttcgatttcc aacagctctt tcatcggcac ataggccagt tcttccagcg
    24121 tcgagaaacc ttcttctacc agaacagtcg cgaagtcttc gtcgatgtcg agatatttgg
    24181 tgaaggtgtc gatcgctgcg tgcgcttccg cctgatgctt agcctgcagg tcgtcaacgg
    24241 tcattacgtt gagttcccaa ccgctcagct gcgaagccag acgcacgttc tgaccgttac
    24301 ggccaatcgc ctgcgccagg ttaccggctt caacggcgat atccatggtg tgtttatctt
    24361 catccaccac gatagaagca acgtctgccg gtgccattgc gttaatcaca aactgcgccg
    24421 ggttatcatc ccacaggacg atatcgatac gctcgccgcc cagttcagta gaaaccgcct
    24481 gaacacgcgc gccacgcata cctacgcaag cacctaccgg atcgatacgt ttatcgttgg
    24541 ttttcaccgc gattttcgca cgagaacccg ggtcgcgagc cgctgcttta atttcaatca
    24601 cttcttcgcc gatttccggc acttcaatac ggaacagttc gatcagcatt tccggcttgg
    24661 aacgagtgac gaacagttgc gcgccacgcg cttccgggcg aacggaataa agcacgccac
    24721 gaacgcggtc gccagggcgg aagttttcac gcggcagcat atcttcgcgc aggatcacgg
    24781 cttcagcatt gttgcccaga tccagagaga tgttgtcgcg gtttactttt ttcaccacgc
    24841 cggtgatgat ttcaccttcg tgttcacgga actgatcaac caccatcgca cgttcggctt
    24901 cacgcacttt ctgcacgata acctgttttg ccgtctgggt ggtgatacgg tcaaaggtaa
    24961 cagactcaat ctgatcttca acgtaatcgc ccaagttcag gctttcatct tcataacgtg
    25021 cggcttcaag ggtgatttcc ttggtcggct gggtgacttc atcaacaact aaccagcgac
    25081 ggaaggtgtc aaaatcaccg cttttgcgat cgatctgtac acggacgtcg atctcttgct
    25141 catatttttt ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc
    25201 gaggtagcgc cttttcattg gatacggctt caactacagc caaaatttct ttgttcatcg
    25261 cgggcttttc acctcatcca gactattaaa agtggggaac caggttcgcc ttctggatat
    25321 tactcagcgc gaacacttca tctttacctt cgacggtaac tgtgatcatt tcaccgtcta
    25381 ccgctttgat aacgccctgc catttacgac ggttttgtac cgccatacgg agaaccagag
    25441 tcacctcttc tccgacaaaa cgggcgtagt gttcagccgt gaacagtggg cgatcgagac
    25501 ccggtgagga gacttccagg ttataagcaa cggtgatggg atcttcaaca tccagcacag
    25561 cacttacctg gtggctcaca tcagcacaat catcaacatt gatgccatct tcactatcaa
    25621 tatagatgcg cagtgtggat gtgcgaccgc gaataaattc gatgcctacc agttcaaaac
    25681 ccagggcctc aactggcgca gtaatcatct ctgttaattt ttgctctaat gtggacaagc
    25741 ccacccccaa gacataaaaa aagggcctaa agcccagtta ttctgtagtc agataacaaa
    25801 aaaccccgat aaatcggggc tttatataac tgaaccctat aaccgcaact gcggtctgga
    25861 gcactttcca gaaggatttt ttcaaatccc actacgaagg ccgaagtctt cacagtatat
    25921 ttgaaaaagg actctaaggg aaagtggttg cgggggccgg atttgaaccg acgaccttcg
    25981 ggttatgagc ccgacgagct accaggctgc tccaccccgc gcctgaaacg tggcaaattc
    26041 tactcgtttt gggtaaaaaa tgcaaatact gctgggattt ggtgtaccga gacgggacgt
    26101 aaaatctgca ggcattatag tgatccatgc cacattttgt caacgtttat tgctaatcac
    26161 gtgaatgaat atccagttca ctttcatttg ttaaatactt ttgccttccc ctgctctccc
    26221 ttaagcgcat tattttacaa aaaacacact aaactcttcc tgtctccgat aaaagatgat
    26281 taaatgaaaa ctcatttatt ttgcataaaa attcagtgag agcggaaatc caggctcatc
    26341 atcagttaat taagcagggt gttattttat gacgacgatt ctcaagcatc tcccggtagg
    26401 tcaacgtatt ggtatcgctt tttccggcgg tctggacacc agtgccgcac tgctgtggat
    26461 gcgacaaaag ggagcggttc cttatgcata tactgcaaac ctaggccagc cagacgaaga
    26521 ggattatgat gcgatccctc gtcgtgccat ggaatacggc gcggagaacg cacgtctgat
    26581 cgactgccgc aaacaactgg tggccgaagg tattgccgct attcagtgtg gcgcatttca
    26641 taacaccacc ggcggcctga cctatttcaa cacgacgccg ctgggccgcg ccgtgactgg
    26701 taccatgctg gttgctgcga tgaaagaaga tggcgtgaat atctggggtg acggtagcac
    26761 ctataaagga aacgatatcg aacgtttcta tcgttatggt ctgctgacca atgctgaact
    26821 gcagatttac aaaccgtggc ttgatactga ctttattgat gaactgggcg gccgtcatga
    26881 gatgtctgaa tttatgattg cctgcggttt cgactacaaa atgtctgtcg aaaaagccta
    26941 ctccacagac tccaacatgc ttggtgcaac gcatgaagcg aaggatctgg aatacctcaa
    27001 ctccagcgtc aaaatcgtca acccgattat gggcgtgaaa ttctgggatg agagcgtgaa
    27061 gatcccggca gaagaagtca cagtacgctt tgagcaaggt catccggttg cgctgaacgg
    27121 taaaaccttt agcgacgacg tagaaatgat gctggaagct aaccgcatcg gcggtcgtca
    27181 cggcctgggc atgagcgacc agattgagaa ccgtatcatc gaagcgaaaa gccgtggtat
    27241 ttacgaagct ccggggatgg cactgctgca cattgcgtat gaacgtctgt tgaccggtat
    27301 tcacaacgaa gacaccattg agcagtatca cgcgcatggt cgtcagttgg gccgtctgct
    27361 gtaccagggg cgttggtttg actcccaggc gctgatgctg cgtgactctc tgcaacgctg
    27421 ggttgccagc cagatcactg gtgaagttac cctggagctg cgccgtggga acgattattc
    27481 aatcctgaat accgtctcag agaacctgac ctacaagcca gagcgtctga cgatggaaaa
    27541 aggcgactcg gtgttctcgc cagatgatcg tattggtcaa ttgaccatgc gtaatctgga
    27601 tatcactgat acccgagaga aactttttgg ttatgccaaa actggcctgc tttcctcctc
    27661 tgccacttca ggcctgccgc aggtggagaa tatggaaaac aaaggccagt aattcgacat
    27721 caaccctgcc cttcaggggt ggggttacct tgcttatcct ctgtaaactc ctttctgagc
    27781 atcacataat cgaaaacatc atggtttgca tccacaaccc gtgagtctcc tttatagtgt
    27841 gcaatcactt cctcaagcgt ttgcggctgt tccggctttg ttacccccat ccacgcatta
    27901 ataaggtaat tattgtaagc tgtcgaaaag atattgtttt ccgttgtgcg atccacgcca
    27961 tccccaagaa cggggctata ccagataaac atcgggacat gatatgcctg ctggctagcc
    28021 tccctgcctc catgaaaata gacgttcttc ttcgtagggt cacgttccag gccgtggtcg
    28081 gcaaaataca tgaccgaggc gcggcgatct tttaataatt caaaaacctg acctagcaaa
    28141 ctatctgtgt aatgaatgga gttgtcatag caggcatcct gatcgtcctg cggttgaaac
    28201 acggcgctgg attgcggata ggcgctacaa gccggttcat ggcttccatt taaatgaaga
    28261 acaatcagtt tcttttgctg cgtattttgc tgtaatgctt gcgataaatg cggcaacaac
    28321 aattcatcaa atcctctgac ataaactgtt tccatggcgc gcatggcgat actggtaact
    28381 gctgtaccat tctgccgaaa agcggattgc gagcttagcc agaaagtctg aaatcctgct
    28441 tgattagcca tattaataat gttgtcgggg taattatgaa tgtcatgact caaaacagaa
    28501 tcagcagtta aagaaagggg aaccgacagc gcagtgtaag gtgcgccgct tattgcttga
    28561 ttaaacagtt tgatctgttt tctttgtgct tcaacttgcg gtgtcgtaga gcgtgtatat
    28621 ccatacaaag acatattgtc gacacgtaca gactccccca caatcaacac gtaggtatca
    28681 atacttgtat ccctgactga taattgaaaa tacggcaccg tgtttgcaat tgagagtaat
    28741 ctttgatgct ctttcgctgc taaagcaaaa tagttgagat tgaaaaacgg cgtataggta
    28801 gcaaatcacg acgctagtat atatggactg aacgcttttt tattttttgc atctttataa
    28861 gcaaattgac aagcggaaaa taaactgccc gaaatgacaa tcagcaataa tattcctgtc
    28921 actttttttg tcggcaagga aacatcatat tttattatta ctgccaaaaa aagaagggat
    28981 aaaaaagcaa aagcacatag ataagggata tacatcccca gcattttcac aacttcatcc
    29041 ggatcactct gcagcacact aatcgcaaat ccatcattga aagttgtacc aaaggtacac
    29101 catgaataga ggctaatact catgtctgcc gcagtcagta acacaaatgg tgcggcaaca
    29161 agcaggcgaa gcggaaggga tgatatacgt ttaacaacca ggatggtcag gacgaagaac
    29221 agcaatcgct gaaccatatg cgctccggaa gaggcgacta agttcgtaat accaaaaaca
    29281 ataacacaaa gatacaacaa ccaatgagat ttaaaatctt ctagcaaatt tgttgaatac
    29341 tgtcatccag aaatccaagt tcacaaccaa tataaacaat tatcaacaat gttcaatttt
    29401 atatcagaca tgtttttcaa ctctgaacca tatcagaccg aaacactgtt agttttttct
    29461 ttttaaatga tgcgaccatt gcgttctcag taattaaata acattatcaa tgcgtctcta
    29521 aacattacgc agaacgtagc catcagtagt tatactgaca ggagcaggat ggtttatcga
    29581 gaagtaaaca tatccgtatt cgtaccggaa ctggagttga cacaataaag tgccaattat
    29641 gtcagcagta gggaaaagtt tataactaag gcgtaccggc accatcgttt caaggtacca
    29701 gctacgagta aagcaactgg acgagataca gatacctgac aaccattcat cagaccagga
    29761 ccaaaacgaa aagagacgct tttcagcgtc tcttttctgg aatttggtac cgaggacggg
    29821 acttgaaccc gtaagcccta ttgggcacta ccacctcaag gtagcgtgtc taccaattcc
    29881 accacctcgg cacggatact actttttagt tcgggatatc gctggtcggc ttagccggag
    29941 cagctggctg agtttgttcg gttttcgccg gtgtactcag attttcccat tcgctacctc
    30001 tattggtttt gttgctattg atgttaccca gcaccagact gatgatgaag aataacgttg
    30061 ccagcagcgc cgtcatgcgg gtcatgaagt taccagaacc acttgaacca aacagcgtag
    30121 cggaagcgcc tgctccgaag gaggctccca tatcagcgcc tttaccttgc tgcagcatga
    30181 tcagaccaac aaggccaatt gccacaataa ggaaaactac taaaagagct tcgtacataa
    30241 tcaacctgtt ccttgcggag ttgccgcgta ccaatgcttc aaccaataaa gcgggagttt
    30301 ttaatctttc ccactgaagc gggtgtgaat actaaccaaa gcgaatgacc ttcgcaaggg
    30361 caattttatc gcattgtatc aactgcggaa aaaaacagca aaaccgatgt gttcgccgag
    30421 aaaaaaggcg acaatcgccg cctttttagc cagttatcta acgctttaaa cggcttttac
    30481 tgcatcggcg atgcggtgtg caaattcagt cacctgcgct tcgtcttcgc cttccaccat
    30541 cacgcgaatt aacggttcgg tgccggattt acgcagcaac acgcgtccac ggttgcccag
    30601 cgcagcttca acctctgcgg tcacggcttt aactgactca tgctcaagcg gatcgccgct
    30661 acctgcggtg taacgtacgt taaccagaat ctgcgggaac attttcatgc cgctgcaaag
    30721 gtcgtgcagg ctcatatggt tacgtgccat cgccgccagc acctgcaagc cagcaacgat
    30781 gccgtcaccg gtagtggttt tatccagcag gatcacatga ccggaattct ctgcaccgat
    30841 acgccagcct ttctcctgca ttttttccag tacgtagcgg tcacccactt tcgcgcgcgc
    30901 aaatggaatt cctaactgtt tcagcgccag ttcaagcccc atgttgctca tcaatgtacc
    30961 cacagcgcca ccacgcagct ggccctgacg aagaccttca cgcgcgatga tatacatgat
    31021 ctgatcgcca tcgactttat tgccttcatg gtcaaccata atcacgcgat cgccgtcgcc
    31081 gtcgaaggca ataccgagat ccgctttttc agccagcaca cgagcctgga gcgcgcgaac
    31141 gtcggtagcc cccacttcgg cattgatgtt tacaccgttt ggctcacaac cgatagcgat
    31201 aacgttcgcc cccagttcgc gcagcacgtt cggcgcgatg tgataagtcg caccgtttgc
    31261 acaatccacc acaatcttca gttcactgag gctaagttcg ttcgggaacg tggctttgca
    31321 aaactcgata tagcgacccg cggcatcaac gatacggctg gctttaccca gttctgccga
    31381 atcaacgcag ctgatctcct tttccatttc cgcttcgatg gcctcttcta ccgcatccgg
    31441 cagtttagtg ccgtcgatag agaagaattt aatcccatta tcgtagaacg ggttatgcga
    31501 tgcagatatc acaattccgg cctctgcgcg gaaggtacgc gtcagataag ccacggccgg
    31561 tgttggcatc gggccagtga agagtgcgga aaggcccgct gccgccagac ccgcttccag
    31621 tgctgactcc agcatatagc cagaaatacg cgtgtcttta ccaataataa tcttacggga
    31681 gccgtggcgc gccagcactt tacccgcggc ccaacccagc ttaagcacaa aatcaggtgt
    31741 gatcggcgca tcccctacac gaccacgaat cccatcggta ccgaaatatt tacgattact
    31801 catagcgttt gttttccttt gcagacagag tggcttccac cacccgcatc gcttctacgg
    31861 tttctttgac gtcatgaaca cgaatgatgt gcgcgccttg cattgcggca atgaccgcac
    31921 aggccagact accgctcagg cgctcggacg gccccacgtt cagcagctgc ccaatcatcg
    31981 attttcgtga catacccacc aacagcggca ggttgaaatg gtgaaattca gccaggcgcg
    32041 ccagtaatga atagttatgg gagagatttt taccgaaacc gaatccgggg tcgagcaaca
    32101 atttctcttt tgcgataccc gcctgctcgc aacgtgctat ttgctcaata aagtagcgat
    32161 tcacttctgc aaagacatcg tcatacttcg gagcttcctg catggttttt ggatttccct
    32221 gcatatgcat cagacaaacc ggtaaaccgg tttctgcagc cgcctccaga gcgccaggtt
    32281 cggaaaggga gcggatatca ttaataatgt gagcgccaac tttcgctgac tcacggatga
    32341 cttctggttt ggatgtatcg accgagatcc agacttcgaa gcgttgagca attgcctcaa
    32401 ccacaggaat aacacgttgc aactcttctt caacgctaac ttccgccgcc cctgggcgcg
    32461 tggactcgcc accaacgtca atgatcgtcg cgccagcatt gatcatcaga ttcgcatgtt
    32521 tcaccgcatc tatcagcgag ttatgcgtgc caccatccga aaaggaatca ggcgtgacgt
    32581 tgaggatccc cattacgtga ggatggctaa ggtccagtga agtaccctgg gcaaagagtt
    32641 tcatgatgtt atccctggta tgaattgata agaaaaaacc ccggagcacg ccccggggtt
    32701 ttcggtacaa atacagtcat ctgatgcggg aacttacttg tcgcctaact gctctgacat
    32761 ggtgttaccc gggttcggcg tacgcggttc atcaaccgga cgaggagcct ttggactacc
    32821 attgtcgcca gaattgttag aagcgcctgg ttcttcccag cccgctggcg gacgtacatc
    32881 gcgacgtgcc atcaggtcat caatctgcgg tgcgtcgata gtctcatatt tcatgagagc
    32941 atctttcatc gcatgcagaa tatccatatt gtcggtcaga agctgacgcg cacgattata
    33001 gttacgctca atcagtgctt tcacttcctg gtcgatgata cgtgcagttt catcggacat
    33061 atgtttcgct ttcgctacgc tacggccgag gaatacttca ccttcttctt ccgcgtacag
    33121 cagtggaccc aatttctcag agaagcccca ctgagtcacc atgttacgtg ccaggttggt
    33181 cgcaacttta atatcgttgg acgcaccggt agaaacatgt tccggcccgt agatgatctc
    33241 ttctgccaga cgaccaccgt acagcgtaga aatctggctt tccagtttct gacggctggc
    33301 gctgattgcg tcgccctcag gcaagaagaa agtcacaccc agcgcacgac cgcgtgggat
    33361 aatcgtcact ttatgcaccg gatcgtgttc cggcaccagg cgaccgataa tcgcatgacc
    33421 cgcttcgtgg taagccgttg attctttctg cgcttccgtc atcaccatgg agcgacgttc
    33481 cgcacccatc atgattttgt ctttcgcttt ctcgaactca accatcgaca caacgcgttt
    33541 gttgccacga gcagcgaaca gtgccgcttc gttcaccagg ttcgccaggt cagcaccgga
    33601 gaaaccagga gtaccacggg caatgattgc cgcgtcgata tcgggtgcca atggtacgcg
    33661 acgcatgtga actttcagga tctgctcacg accgcgaaca tctggcaagc cgaccacaac
    33721 ctggcggtcg aaacggccag gacgcagcag ggccgggtcg agaacgtccg gacggttagt
    33781 cgcggcgata acgatgatac cttcgttacc ttcgaagcca tccatctcaa ccagcatctg
    33841 gttcagagtc tgttcacgtt catcgtgacc accgcccaga ccagcgccac gctggcggcc
    33901 tacggcgtcg atttcatcga taaagatgat gcacggtgcc gctttcttcg cctgttcgaa
    33961 catgtcacga acacgggatg cacccacacc gacgaacatt tctacgaagt cagaaccgga
    34021 gatagtaaag aacggaactt tcgcttcgcc tgcaatcgct ttcgccagca acgttttacc
    34081 ggtacccgga ggaccgacca tcaggacgcc tttcgggatc ttaccgccga gtttctggaa
    34141 gcggctcggc tcgcgcagat actctaccag ttcagcaact tcttcttttg cttcgtcgca
    34201 gcccgcaacg tcagcaaagg tcgttttgat ctgatcttcc gtcagcatgc gcgctttgct
    34261 cttaccaaac gacatggcac ctttgccacc gccgccctgc atttgacgca tgaagaagat
    34321 ccagacacca atcagcaaca gcatcgggaa ccaggagatg aagatagaag ccagcaggct
    34381 tggttcttca ggcggttcac cgacaacctt gacgttcttg gtcaacaggt tatccagtaa
    34441 tttcggatcc tgaaccggaa tgtaagtggt ataacggtta ctatctttct tggtaacgtt
    34501 gatttcacgt ccgttgatac gcgcttcacg aacctggtcg ttattgacct cttgtaggaa
    34561 ggtagagtaa tccaccttac ggccattaga ctcgctgggc ccaaagctct ggaatactga
    34621 catcagcaca acggcaatga ccagccagag tattaggttt ttcgccatgt cactcaaggg
    34681 attaacctct tattacaact gtgttaaaaa cagcgtcagg atactctata tccagcatct
    34741 ttcaaacttt cgtctgaaat ctcccggtta gggtttacgc ccggtcgcta caatatacac
    34801 ttctcgcgaa cgtgcacgag aagagtccgg cttacgaact ttgaccttcg taaacaggga
    34861 gcgaatttcc cttagatact catcgaaacc ttcgccctgg aacaccttca ctacaaaact
    34921 gccacctggc gctaatacat cacgacacat ttctagcgcc agttccacca gatacatggc
    34981 acgggggata tccaccgccg gtgttccgct catgtttggt gccatatcgg acatgacaac
    35041 ctggactttg ctgtcgccaa cgcgctccag cagtgctttc atcaccagtt catcacgaaa
    35101 atcgccctga agaaagtcca caccaacgat aggatccata ggtaaaagat cgcaagcgat
    35161 gatgcggcct ttgccgccaa tttgggtgac cacatattgt gaccaaccac ccggagcagc
    35221 accaaggtcg acaaccgtca ttcccggttt aaagagtttg tcactttgct gtatttcatc
    35281 aagtttaaac caggcacggg aacgtaatcc ctttttctgt gcctgttgaa catatttatc
    35341 gctaaagtgt tcctgaagcc agcggctgga gctggcagaa cgcttcttac ctgtcattta
    35401 actttcccat ggggataact catcgtaacc aattgcgtaa atttttacgc gcctatttgg
    35461 tgatatatgg gagatggcgg tagaatgacc cgttttcaat cccaacgtaa gcaaaaaata
    35521 cgatgaatct gagtactaaa caaaaacagc acctgaaagg tctggcacat ccgctcaagc
    35581 cagttgttct gcttggcagt aatggtttga ccgaaggggt gctggccgag attgaacaag
    35641 cgttagagca ccatgaactc atcaaggtga aaatcgccac cgaagatcgc gaaacaaaaa
    35701 ccttgatcgt ggaagctatc gtgcgcgaaa ccggcgcctg taatgtacag gtcatcggta
    35761 aaacgctggt gctttatcgc ccaactaaag aacgtaaaat ctcgctgcca cgctaagatt
    35821 atcctaaagt tacacacatt tgctgtgtaa aacgaggggt tttccgcagg caggagagca
    35881 aaatgccacg ctctgttcgt tgataaaagg ccgcagagcg gcctttttcc tttctttaca
    35941 atacatcaac atcttgagta ttgggtaatt cttacaggta ttccacctta attacttcaa
    36001 attctacttc gccgcccggc gttttgatga ccacaacatc atcttcttct ttgccgatca
    36061 ggccacgagc aataggcgag tttacagaaa tcaggttttg tttaaagtca gcttcgtcat
    36121 cgccaacgat gcgataagtc tgttcttcgt cagaatccag attcagcacc gttacggtag
    36181 caccaaaaat aacgcgccca ttgttgggca ttttggtgac atcaatcacc tgcgcgtttg
    36241 acagcttggc ttcgatgtct ttgatacggc cttcgcagaa gccctgctgt tcacgagctg
    36301 cgtggtattc ggcgttttct ttcaggtcgc catgctcacg cgcttccgcg atagcagcaa
    36361 tgatttcagg acggcgcaca gatttcagaa aatccagctc ttcgcgtaat ttttcagcgc
    36421 cgcgtaaggt catcggaata gcttgcattt gttatacctc ttgaatattc ctgatggggc
    36481 aagtcttcac ccaccccggc tgttcggcct gcccggcata actcctcacc gggaccagaa
    36541 gcaaaaaaat accgacccgg gtacaagtcc caggtcagct acaattcaca ttttgatggt
    36601 cattttaccc tgaagttccc gaagggtcat cgtttacttt atagggcgtt gcgccgtagt
    36661 atgacggctc gattccaggt tgttagcgcg agattatgcg attttccaga tttatcatcg
    36721 gattgaccag ctgtatagcg ttcagtgttc aggccgcgaa tgttgatgag tacgttactc
    36781 aactccccgc aggtgccaac cttgccctga tggtgcaaaa agtcggtgcg tcggcccccg
    36841 ctattgatta ccacagtcag cagatggcgc tgcccgccag tacccagaaa gtgattactg
    36901 cgctggcggc gttgattcaa ctcggccccg attttcgttt caccacgacg cttgaaacca
    36961 aaggcaatgt ggaaaacggc atacttaagg gtgacttagt ggcgcgattt ggtgccgatc
    37021 cgacgttaaa acgtcaggat attcgcaata tggtcgcgac tttgaaaaaa tctggcgtca
    37081 accaaatcga tggcaatgtg ttgatagata cctccatttt cgccagccac gataaagccc
    37141 ccggctggcc atggaatgac atgacacaat gctttagcgc tccgcctgct gccgccatag
    37201 ttgaccgcaa ctgtttctcc gtctcgctct acagtgcccc aaagcctggt gatatggctt
    37261 ttatacgcgt ggcatcttat taccccgtta cgatgttcag ccaggtacgc accctccccc
    37321 gtggttctgc cgaagcgcaa tactgcgaac tggatgtggt gccaggcgac ctgaaccgct
    37381 ttacgctgac gggatgcctg ccacaacgtt ctgagccgct cccgttggct tttggcgtgc
    37441 aggatggagc cagctatgcc ggtgcaattc tgaaagatga gttaaaacag gcgggtatca
    37501 cctggagcgg aacactgctg cgccagactc aggttaacga acctggaacg gtagttgcca
    37561 gtaaacagtc ggccccgctg cacgatctgc ttaagattat gctgaaaaag tcggacaaca
    37621 tgattgccga tacggttttc cgcatgatcg gccatgcgcg cttcaatgtg cctggaacat
    37681 ggcgggctgg gtcggacgcc gtgcgtcaga tcctgcgcca gcaagccggt gtcgatattg
    37741 gtaacaccat tattgccgat ggttcagggc tttcgcggca taacctgatt gcccccgcca
    37801 ccatgatgca ggtgctgcaa tacattgccc aacacgacaa tgaacttaac tttatctcca
    37861 tgctgccgct tgcgggctat gacggctctt tgcagtaccg tgcaggtctg catcaggcgg
    37921 gcgtggatgg caaagtctca gcgaaaaccg gttcgttgca gggggtatat aacctggcgg
    37981 gattcattac cacagcgagc gggcaacgaa tggcatttgt acaatatctt tctggctatg
    38041 cagtaaaacc cgcggatcag cgtaatcgcc gtattccgtt agtgcgtttt gaaagccgtt
    38101 tgtacaaaga tatttatcag aacaattagt caaaaagaaa ccccggcgca tggccggggt
    38161 ttcagattat tgacaaagtg cgctttgttt atgccggatg cggcgtgaac gccttatccg
    38221 gcctacaaaa tcgtgcaaat tcaatatatt gcaatcatct tgtaggcctg ataagtgtag
    38281 cgcatcaggc ttatttggca ttggtcatta gtgattaacg cttgtaaatg aactcaacgc
    38341 cttcttcgtc gtcttcgtcc cagtcgtcat cccagtcttc atcatcctct tcagcaatct
    38401 cttcaagctg ctgacggtga taatcatccc acatgaattc gactttctct ggctgtttcg
    38461 cttcttcagc ctgcacgacc gggttttcaa tgataaaggt catcacatcc cagcagagat
    38521 ctttcacgcc cagtccgctc gccgcagaga tcagataata tttatcctcc cagcccagcg
    38581 cttcagcgat cgctttcgct ttctcttcgg cttctgcctt atccagcaga tcgatcttgt
    38641 tgaagactaa ccaacgcggt ttcgccgcca gatcctggct gtatttttcc agctcgctga
    38701 taataatacg cgcgttttca accggatcgg tgccgtcaat cggatcgata tcgatgaggt
    38761 gcaacaggac gcggcaacgt tccaggtgct tcaggaagcg aatgcccaga cctgcgcctt
    38821 ccgcagcgcc ttcaatcagt cctggaatat cggcaacaac gaagctcttt tcgttgtcca
    38881 ttcgtaccac acccagactt ggcaccagag tggtaaacgg ataatccgcc actttcggtt
    38941 tagccgccga taccgcacga ataaaggtcg atttacccgc gtttggcatc cccaacatac
    39001 cgacgtcagc cagcagcatc agctccagca gcagctcacg cttatcgccc ggcgtaccgt
    39061 tagttttctg ccgtggtgta cggttaactg acgatttgaa acgggtattg cccagaccgt
    39121 gccagccgcc cttagcaacc agcagacgct gaccgtgttt agtcatatca cccatggttt
    39181 caccagtacc ctggtcgatt acacgcgtac ctaccggcac tttaatcgtc acgtctttac
    39241 cgcgcttacc ggtacaatcg cggcttgcgc cgttctgacc gcgctctgca cggaaagatt
    39301 tttcaaaacg ataatcgata agcgtgttca ggttctcgtc ggcttccatc catacgtcac
    39361 caccatcacc gccgtcgccg ccatccgggc cgcctttcgg aatatacttt tcgcggcgga
    39421 agctcacgca accattaccg ccatcacctg caacgaccag aatcgatgct tcatcaacaa
    39481 acttcatttt attctccgta aatcattcgc ctgcgcgggg ttgcgatacc accgttgtat
    39541 gcttacgtaa tccgccccaa atacgatgac caatggcgga atacatcgcg cccgcaacca
    39601 cgacaaacgc accgagataa cctaaaaggt ttaacatcgg tctggcgaag aaatcaggcc
    39661 aggccagtga taaaagatct gaaaaaaaca gcgtaaacag tggggtgagc gtgatgatcg
    39721 cgctcacctg cgctgcctgc cagcgagcca tcgcttccgc caaggcgcca tatcctacca
    39781 aggtattcag tccgcaaaaa attaaacatg cgagctgcca gtggctaagc tgcgctatca
    39841 ctccaggctt tgccagaggg aagagcgcga ttgtacataa agtgtacagt aaaaacagga
    39901 tctgcggtga ggccagccga cgcaataaaa ccttttgcgc cacgccataa ctcacccaaa
    39961 ccgtcgccgc accgacccca aagataactc cccaggtgta atcggtgagc tttgtaaata
    40021 tctcgaccag actggtgtta aaaaacatca ccagaccgct caggagcatc aatgccccta
    40081 caacctgagt gctgcgcatt ttctctttca ggataaatac gctggcaacc atcatgccaa
    40141 ctggcgagag ttgcccaatc acctgcgaag cggtcggact caggtattgc aaggatgagc
    40201 tgaacaggat gaagttccca aacagcccgg cggtcgccac tgccaacaaa atcaaccagc
    40261 gtggcttacg aaacacgcgt aatggcgaca acctcttctt caccgcaaga atggcaccaa
    40321 ggccaatact cgccatcaag aaacggtaaa acacgattgt cggaggttcc atcacctcca
    40381 gcacctgctt cattgcgatt ggcaacgccc cccagcaaat tgctgtggta agcgccaaaa
    40441 gaatgccaat gcctgcctgc tgcttcatac cgtattccct gcaaagcgca tttgcggatt
    40501 aacgtcgcac catgcgcatt tttaccggtt atcgaatgta aaaagccccg caacgtgttg
    40561 cggggctttc atccgttacc gggacgcgaa aaacttattc agcttcgatg ctgataaatt
    40621 tacggttttt cgggccttta acttcgaatt tcactttacc gtctgctttg gcaaacagag
    40681 tgtggtcacg accgcaacct acgttagcgc cagcgtggaa tttggtacca cgttgacgaa
    40741 cgatgatgct acccgccaga acggattcgc caccgaaacg cttaacgccc aggcgtttag
    40801 cttctgaatc gcgaccgtta cgtgtggagc cgccagcctt tttatgtgcc atttgaaatc
    40861 tctcctcagg tcttaggcgc tgatgccagt aattttcaca tcagtgaacc actgacgatg
    40921 gccctgctgc ttacgatagt gtttacgacg acgaaactta acgattttaa ctttctcgcc
    40981 acgaccgtga gcaacaactt cagctttgat tacgccgcca tcaacgaaag gaacgccgat
    41041 tttgacttct tcaccgtttg cgatcatcag cacttcagcg aactcaacag tttcgccagt
    41101 tgcgatgtcc agcttttcca ggcgaacggt ctgaccttcg cttactcggt gttgtttacc
    41161 accactttgg aaaaccgcgt acatataaaa ctccgcttcc gcgcacacct tttcaatgat
    41221 tcagagtgcg ctataaatat tcacaatagg gcgcgaatat tacgcaaaac gcacgccttt
    41281 gacaagtgct acagtcaata cacgaagaaa aaaaacacaa cttgtacggt aacgtttatc
    41341 tgtgccattt tttcagtaca attaccctat attcctaacc ataaacccta agttgccttt
    41401 gttcacagta aggtaatcgg ggcgaaaagc ccggcttttg cgatgaattt agaaaaaatc
    41461 aatgagttaa ccgcgcaaga tatggcgggt gttaatgcgg caatccttga gcagcttaat
    41521 tccgacgtcc aactgatcaa tcagttaggc tattacatcg tcagcggcgg cggtaaacgt
    41581 attcgtccga tgattgctgt actggctgca cgagctgttg gctatgaggg aaatgcgcat
    41641 gtcaccattg ctgccctgat cgagtttatc cacacggcga ctctgctaca cgacgacgtt
    41701 gtggatgaat cagatatgcg caggggtaaa gctaccgcca acgccgcatt tggcaatgcc
    41761 gccagcgtgc tggtaggcga ttttatttat acccgcgcgt tccagatgat gaccagcctc
    41821 ggttcactca aagtgctgga agtcatgtca gaagctgtaa acgtcatcgc agaaggtgaa
    41881 gttctgcaac tgatgaacgt taacgatccg gacatcactg aagaaaacta catgcgcgtt
    41941 atctatagca aaaccgcgcg tctgtttgag gctgccgcgc agtgttccgg gattctggct
    42001 ggctgtacgc cggaggagga gaaaggcctg caggattatg ggcgctatct cggcactgct
    42061 ttccagttga tcgacgattt actcgattac aatgccgatg gcgaacagtt aggtaaaaac
    42121 gtcggcgacg atctgaacga aggtaaaccg acgctgccgc tgctgcatgc gatgcatcat
    42181 ggcacaccag aacaggcaca gatgatccgt accgccatcg aacaaggtaa cggtcgccat
    42241 cttctggaac cggttctgga agcaatgaac gcttgtggat ctcttgaatg gacgcgtcag
    42301 cgcgccgagg aagaagcaga caaagccatc gcagcgttac aggtgctccc ggacacccct
    42361 tggcgagaag cactcatcgg cctcgcgcac atcgctgttc aacgcgatcg ttaatcccct
    42421 cccttcatcc cgcgcagtgc gcgggatgat tccagtaaat tccataaaaa cactaattca
    42481 gcgctataac acctgaaaaa aagggcgatg aatctttata tattctgaat attcacactc
    42541 tttacaggaa ctttttagcg caataggcca tcaggagtat agtgatgccc gacagaagaa
    42601 gtgttctgaa tgaaagcgac taacttaagg agtgaggaaa gatggaaagt aatttcattg
    42661 actggcatcc cgctgacatc attgcaggtt tgcgcaagaa aggaacgtca atggcggcgg
    42721 aatctcgcag aaatggtttg agttcctcaa cgctggcaaa cgcattatcg cgcccatggc
    42781 cgaaaggaga gatgattatt gcgaaagccc tgggaactga cccctgggtt atctggccat
    42841 cacgctacca tgatccgcgg acccatgagt ttatcgacag aacgcagttg atgcgtagct
    42901 acactaaacc gaaaaaatga gtggtctggc ggtagccccg cgaacggggc tgccagctct
    42961 cagacgatta ctcgcctttc acacgctcaa tatttgcacc taaagcgcgc agtttgtctt
    43021 caatgcgttc gtagccacga tcgatgtgat aaatacgatc aaccaccgtc gtcccttccg
    43081 caatacagcc agccagcacc aggcttgctg atgcacgcag atcggttgcc ataacctgtg
    43141 caccagaaag tttttcaaca ccatgacaaa taacggtatt gctttcgatt tcggcgtgcg
    43201 cgcccatacg gctcagctct ggcacatgca taaagcggtt ttcaaagacc gtttcggtga
    43261 tgaacccggt cccttcagcc accaggttca acagcgtgaa ctgggcctgc atatcggtcg
    43321 ggaatgccgg atgcggcgcg gtacgtacgt taacagcctt cggacgtttg ccatgcatat
    43381 ccaggctaat ccagtcttcg ccgacttcga tgtccgctcc agcatcacgc agtttcgcca
    43441 gcacggcgtc cagagtatct ggctgcgcgt tacggcagat aattttgcca cgagagatcg
    43501 ccgccgccac caggaaagta ccggtttcga tacgatccgg cagcacgcga tagacaccgc
    43561 cgcctaaacg ttccacacct tcgatgacga tacgatcggt gccctgaccg ctaattttcg
    43621 cacccagcgt aatcaggaag ttcgcggtat cgacgatttc cggttcacgc gctgcgtttt
    43681 caataatcgt ggtgccttcc gccagggttg cagcacacat gatggtcacc gttgcgccaa
    43741 cgctgacttt atccatcacg atatgtgcgc ctttcagacg accatcgacg gaagctttaa
    43801 cgtaaccttc ttccagtttg atggtcgcgc ctaattgttc gaggccagaa atgtgtagat
    43861 caaccggacg agcaccgatc gtacaaccgc caggtagtga aacttgcccc tgaccaaagc
    43921 gcgctaccag cggccccagc gcccagatag aagcacgcat ggttttaacc agatcgtaag
    43981 gtgcgcagaa tacattaacg tcgcgggcat caatatgcac agaaccatta cgttctactt
    44041 tcgcacccag ctggcttagc agtttcattg atgtatcgac gtctttcagt ttcgggacgt
    44101 tctggatctc taccggttct tccgccagta gtgcggcaaa aagaataggc agagcagcat
    44161 ttttagcgcc ggaaattgtg acttcgccct ggagcttcgt tggcccctga acacgaaatt
    44221 tatccattta gtttgttctc agttaacaat tcatatccgc taccggcgaa tcgcccatag
    44281 ctcaaaagcc gttcagtttg cgatcgcgcg cccactccgc aggggtatac gctttgatcg
    44341 acacagcatg aatgcggtta tccgcaatat attccatcag cgggccatag accgtctgct
    44401 gttttttaac ccgactcata ccgtcaaaca actcacccac ggcaataacc tgaaagtgac
    44461 tgccatcgcc ggaaacgtgg acttcctgga gggagagagc gttcatcaac acgctctgaa
    44521 tttcattatt ttccatggga tcttcaatca tcagttaata aaccagcgaa acatcttaga
    44581 gcaaagttgc gctggcataa ataagcaaaa agcctcgctg ataaatcaga caaggctcga
    44641 cttgcaggca ggtttgccgg acaggcggtt aacgccatat ccggcctgaa aaaatttaac
    44701 gaggcagaac atcagcaggc aaattataca atttcgcgag ggtatacact ttgtcgttta
    44761 ccccctgaag cgtcacgttg ttgccctgct ttttcgccag atcgataaga tggagcagca
    44821 gtgccagtcc ccccgtatcc acgcgggaca cacggctaag atcgatgcag gtaatcccct
    44881 tcaccgcttc ctcacgcatt tcccaaagcg gtagcagaac gtcctgatcc agctctccgg
    44941 ataacgccag cgtgtcaccc gtctgcatcc agctcagtga ctcgctcatt attttttctc
    45001 ttccagagtg attttctgtt gagaaatcga tttcagttgc gcagtcaggc cgtcgatacc
    45061 tttggtacgc agcagcgttc cccactcgtt ttgtttggtg gtgatcatac tgacgccttc
    45121 agcaatcatg tcgtaagctt gccagtttcc cgtctgggag tttttacgcc actggaagtc
    45181 cagacgcacc ggcggacggc cattcgggtc aataatggta acgcgaatag gcacaatggt
    45241 tttatcgccc agcggctgtt ctggcgcaat ctgataggtt tgaccgtgat acatcgccag
    45301 cgcctgaccg taagcctgct tcaggtactc acggaaagcg gcaaagtagg cttcacgttg
    45361 agcaggggtc gcactcttgt aatactggcc cagcaccagc gcaccggcgt atttcacctg
    45421 tacgtatggc agcagttcct gatcaacaat ggtacgcaga taatccgggt tggcccgaat
    45481 ttgcggttgc tcattcttca ggcgatcgaa cgttttctgc gccgcctcgt ccatcagctt
    45541 ataaggattg gtctggtctg ccgcggttgc cgcactcaga ggtgcaatca ccagcaaagc
    45601 gaccatcatt aaacgtttaa acatgcgtcg gttctcctga aattatttcg ttgtacccac
    45661 aggttcagtg gtttcattat tacctggcgc agcagctggc gcatcgccac tattcttatt
    45721 gtcatcgcct ttactaccgt aaaggaactg accaatgaga tcttccagca ccatcgcaga
    45781 cttggtgtcc tgaattgtat cgccatcctt caggatagca gtccccagtt ccgggtcttc
    45841 aaaaccgacg tttaatgcca gatattgttc tcccagcagg ccggaagtac gaatgctcag
    45901 cgaactggta tcaggaatgt ggttataacg ttgttcaatt tccagcgtta cgcgcggcag
    45961 ataggttttc gggtccagcg taatatccgc cacccggccc acaacaacac caccaatgct
    46021 gaccggagag cgcgctttca ggccgccaat gttatcgaac gtcgcataaa gcgtgtaggt
    46081 cgattcagta cgtatggacg tcacgttcgc cgccttcagg caaacaaaca gcgccgccag
    46141 cagtgctgct aataaaaaga tacccaccca aatttcattt tttttcgttt gcatgaactc
    46201 aattcccaaa catcaatgcg gtcagcacaa aatccagccc cagaacagcc agagacgagt
    46261 ggacaacggt gcgagtggtt gcccggctaa tcccggcaga cgtcgggatg gcgtcgtagc
    46321 cgttaaacaa cgaaatccac gtcaccgtga tggcgaacac cacgctctta atcagacagt
    46381 tgaccagatc catacgccag tcgacggcat tttgcattgc cgaccagaag aacccgctat
    46441 caatgccttt ccaactgacg ccgaccagcg atccgcccca gatccccacg gcgacgaaga
    46501 taaccgtcaa cagtggtaat gaaataaccc cagcccagaa acggggagaa ataacccgac
    46561 gcagcggatc caccgccatc atctccatac tggagagttg ctctgtagcg cgcatcaggc
    46621 cgatttctgc ggttaacgcc gaaccagcac gcccggcaaa caacagcgcg gcaaccaccg
    46681 gccccagttc acgcagtagc gataacgcca ccagcatacc cagactggtt tccgcgctat
    46741 aagtggtcag aaccagataa ccttgcagcc ccaacaccat tccgatgaac acgccagaaa
    46801 ctacaataat cagcatcgac aggacgccga cattatagag ctggcgcacc agcagcggca
    46861 catgtttgcg aaattccggt ttgccgacca gcgcattgaa taacattaac ccggcccgcc
    46921 cgaacgttct cagggtttta atccctttat gtccgagcga cgccagcgca tttaacagca
    46981 tgagtggctt aactccctgg taaaagatca gcgtgataat cgccggcagg atagcggaac
    47041 ggaacaggcc cgtcagctat cccgtccaga aactgacgta cgcgcggatc aggattcgtc
    47101 tgcaacgcct gggcactgcc atgagcgaca atttttttgt ccgccaggat ccaggcgtga
    47161 tccgcaatac ttaacacttc cggcacatcg tgagaaacca ccacacaagt cacgcccagc
    47221 gcgctgttca gctcagaaat cagcttcacc agtacgccca tggtgatggg atcttgccca
    47281 acaaaaggtt catcaaacat gatgagatcc ggctccagcg caatcgcacg cgccagcgct
    47341 gcacgccgcg ccatcccacc ggaaagttca gaaggcatta gtttagccgc tccacgcagc
    47401 cccacggcct ccagcttcat catcaccgta ctatgcaaca atggcgcggg aagttgggta
    47461 tgttcgcgca gtggataggc gacgttgtca aatacgttca tatcagtgaa caacgccccg
    47521 gactgaaata acatgctcat ccgtttacgc actgtataca ggcgcgaacg agacatcgcc
    47581 ggaatattct caccatcgaa aaggatctca ccatgatctg gtgcgatttg cccgccaatc
    47641 agacggagta gcgtcgtttt accgatgccc gatggcccca tgatcgccgt gatcttccct
    47701 cgcggcacgg tcagggaaat attatcgaag atgcagcgat tgccacgcgt aaaactgaca
    47761 tcgcgcatat cgactaaatt cgccacagac tgctccataa ttcacccttc gtcttgcgtt
    47821 gattttctaa gcatggcgct caatttaacc ttgaacccaa catatttaca gaatattacc
    47881 cgccgtggtt agcgaaagct ggcatttgtt ttacttttta gccgcataaa gtcaaaatta
    47941 agaatccgtt acggctttct gaaaatcttc agcggaccgg cgagtatacc tgaagaaagg
    48001 acgttagatg cttttagcta cggcactgtt aattgttggt ttacttttgg tcgtttacag
    48061 tgccgaccgc ctggtttttg ccgcgtctat tctttgccga acctttggca tcccgccgct
    48121 gatcatcggc atgacggtgg tcagtattgg tacatcgtta ccagaaatca tcgtctcgct
    48181 tgccgcgtct ctgcacgaac aacgcgattt agccgttggt acagccctcg gctcaaacat
    48241 tatcaatata ttgctgctcc tcggtctggc tgcgctggtt cgtcctttta ccgtccattc
    48301 cgatgttctg cgccgtgaat tacccttaat gttgttggtc agcgtggtgg ccggttccgt
    48361 actctatgac ggacaactta gtcgaagcga tggtatcttt ctcctctttc tggctgtgct
    48421 atggctgctg ttcattgtta aacttgcacg tcaggggact gacagcctga ccagagagca
    48481 gcttgcagag ctgccgcgtg acggcggatt gcccgtcgcg tttttatggc tcggcattgc
    48541 gcttatcatc atgccagtgg ccacgcggat ggtggttgat aacgccacgg tgctggcgaa
    48601 ttactttgcc atcagcgagt tgacgatggg tctgacggca attgctatcg gaaccagtct
    48661 gccggaactg gcaaccgcaa tagcgggggt tcgcaaaggt gaaaacgaca ttgctgtcgg
    48721 aaatatcatt ggcgcaaaca tttttaatat tgtcatcgtg ttgggtttac ccgcgctgat
    48781 aacgccagga gagattgatc cactggcgta cagtcgtgac tacagcgtga tgttgctggt
    48841 gagcattatt tttgcgttgc tgtgctggcg gcgctccccg caaccgggcc gtggtgtagg
    48901 ggtattatta actggcggat ttatcatatg gctggcgatg ttgtactggt tatcgccaat
    48961 actcgttgaa taactggaaa cgcattatgt cgcacgtaga gttacaaccg ggttttgact
    49021 ttcagcaagc aggtaaagaa gtcctggcga ttgaacgtga atgcctggcg gagcttgatc
    49081 aatacatcaa tcagaatttc acgcttgcct gtgaaaagat gttctggtgt aaagggaaag
    49141 ttgtcgtcat ggggatgggg aaatcggggc acatcgggcg caaaatggcc gcaacgtttg
    49201 ccagcaccgg tacaccttca tttttcgtcc atcctagtga agccgcgcat ggtgatttag
    49261 gcatggtcac cccacaggat gtggtgattg ctatctctaa ctcaggtgaa tccagcgaaa
    49321 tcacggcctt aattccagtg cttaagcgtc ttcacgtacc gttaatctgc atcaccggtc
    49381 gcccggagag cagcatggcg cgcgccgcag atgtgcatct gtgtgttaaa gtagcgaaag
    49441 aagcctgtcc gttagggctg gcaccgacca gcagcaccac cgccacgctg gttatgggcg
    49501 atgccctcgc tgtcgcgctg ttaaaagcac gcggctttac tgctgaagat tttgcgctct
    49561 cacacccagg cggcgcactg ggtcgtaaac ttctgctgcg cgtaaacgat attatgcata
    49621 cgggcgatga gatcccgcat gttaagaaaa cggccagtct gcgtgacgca ttgctggaag
    49681 ttacccgcaa aaatcttggt atgactgtca tttgcgatga caatatgatg attgaaggca
    49741 tctttaccga cggtgattta cgccgtgtct tcgatatggg cgtggatgtt cgtcagttaa
    49801 gtattgccga tgtgatgacg ccggggggaa tacgtgtgcg ccctggcatt ctggccgttg
    49861 aggcactgaa cttaatgcag tcccgccata tcacctccgt gatggttgcc gatggcgacc
    49921 atttactcgg tgtgttacat atgcatgatt tactgcgtgc aggcgtagtg taaagattca
    49981 aggataaaca acaatgagca aagcaggtgc gtcgcttgcg acctgttacg gccctgtcag
    50041 cgccgacgtt atggcaaaag cagagaacat tcgtctgctg atcctcgacg ttgatggcgt
    50101 actgtcagat ggcctgattt atatgggcaa taacggcgaa gagctgaaag cgttcaatgt
    50161 tcgtgacggt tatggcattc gttgtgcgct cacctctgat attgaagtcg ctatcattac
    50221 cgggcgaaag gctaaactgg tagaagatcg ttgtgccaca ttggggatca ctcacttgta
    50281 tcaggggcag tcaaacaaac tgatcgcctt tagcgatttg ctggaaaaat tggcgattgc
    50341 cccggaaaat gtggcttatg ttggcgatga tctcatcgac tgcccggtaa tggaaaaagt
    50401 gggtttaagc gtcgccgtgg ccgatgcgca tccactgttg atcccgcgcg ccgattacgt
    50461 gacgcgcatt gctggcggtc gtggcgcagt gcgcgaagtt tgcgacttat tactcctggc
    50521 gcagggcaaa ctggatgaag ccaaagggca atcgatatga gtaaagccag acgttgggtt
    50581 atcattgtgc tatcactggc ggttctggtg atgatcggca ttaatatggc cgaaaaagac
    50641 gataccgccc aggtggtcgt caacaacaat gatcccacct ataaaagcga gcatacggac
    50701 acgctcgtct ataacccaga aggggcacta agctatcgat tgattgctca acacgttgaa
    50761 tattattccg atcaggccgt ttcgtggttt acgcagccgg tacttaccac gtttgataag
    50821 gataaaatcc cgacatggtc cgtaaaagca gataaagcca agctgaccaa tgaccggatg
    50881 ctctatttat atggacacgt tgaagtcaac gcacttgtgc cagactctca acttcgcaga
    50941 atcacgacgg ataacgcgca gatcaatctg gtgacgcagg atgttacctc tgaagacctc
    51001 gtcacgttat acggaacaac atttaactcc agcggtctga aaatgcgcgg caacttacgc
    51061 agcaagaacg ccgagctgat tgaaaaggtt agaacatcct atgaaattca aaacaaacaa
    51121 actcagcctt aatcttgtgc ttgccagctc acttctggcc gccagcattc cggcatttgc
    51181 cgtaaccgga gacactgatc agccgatcca cattgaatcg gaccagcaat ctcttgatat
    51241 gcaaggcaac gtggttacct ttaccggtaa tgtcatcgtc acccagggca ccatcaaaat
    51301 taatgccgac aaagtggtcg ttacccgtcc gggcggcgaa caaggtaaag aagtgattga
    51361 cggatacggt aaaccggcaa cgttctacca gatgcaggac aacggtaaac ccgttgaagg
    51421 tcacgcttcc cagatgcact acgaactggc aaaagatttt gtcgttctga cgggtaatgc
    51481 ttatctgcag caggtcgata gcaacattaa gggcgataag atcacttacc tggtgaaaga
    51541 gcagaaaatg caggctttca gcgacaaagg caagcgcgta accaccgttc tggtgccgtc
    51601 gcagctgcag gacaaaaaca acaaaggcca gaccccggca cagaagaagg gtaattaatt
    51661 cgttatggca acattaactg caaagaacct tgcaaaagcc tataaaggcc gtcgcgtggt
    51721 agaagacgtc agcctgaccg tcaactccgg ggaaattgtc ggtctgctgg ggccaaacgg
    51781 tgccggtaag accaccactt tctacatggt tgtaggcatt gtgccgcgcg atgcgggcaa
    51841 catcattatt gatgatgacg atatcagtct gctgcctctg catgcacgcg cgcgccgcgg
    51901 tatcggctat ctgccacagg aagcctccat tttccgtcgc ctcagcgttt acgataacct
    51961 gatggcggta ctgcaaattc gtgacgactt gtctgctgaa caacgtgaag accgcgcgaa
    52021 cgagctgatg gaagagtttc acattgagca cctgcgtgac agcatggggc agtcactctc
    52081 cgggggtgaa cgtcgccgtg tagaaattgc ccgcgcactg gctgcgaatc cgaaatttat
    52141 tctgctcgac gaaccgtttg ccggggttga cccgatctcg gttatcgata ttaaacgcat
    52201 cattgaacac ctgcgcgaca gcggcctggg cgtgctgatc actgaccaca acgtgcgtga
    52261 aacactggcg gtttgtgaac gcgcttatat cgtcagtcag gggcatttga tcgcccacgg
    52321 cacgcctaca gaaatcttac aagacgaaca cgttaagcgt gtataccttg gggaagactt
    52381 cagactctga tagggtagaa gtttgcgacg ttttagcagg agagtacgat tctgaacatg
    52441 aagcaaggtt tgcaactcag gcttagccaa caactggcga tgacgccaca gctccaacag
    52501 gcaattcgtc tgttgcagtt gtcgaggttg gaacttcagc aggagctaca gcaggcgctg
    52561 gagagtaatc cgctgcttga gcaaatcgac actcatgaag aaatcgacac ccgcgaaacg
    52621 caagacagtg aaacgctgga caccgccgac gcgctcgaac aaaaagagat gccggaagag
    52681 ctgccgctcg atgccagttg ggacaccatt tacaccgctg gtacaccatc cggcaccagc
    52741 ggtgactaca ttgacgacga gctgccggtc taccagggcg aaacgacgca gaccttgcag
    52801 gattacctga tgtggcaggt cgagctgaca ccgttttccg acactgaccg cgctattgct
    52861 acctctatcg tcgatgccgt tgatgaaacc ggttatctga ctgtcccgct ggaagatatt
    52921 ctcgaaagta tgggcgatga agagatcgac atcgacgagg ttgaagccgt ccttaagcgg
    52981 atccaacggt ttgatccggt cggtgtagcg gcaaaagatc tgcgtgactg cctgctaatc
    53041 caactctccc aattcgataa gaccacgccg tggctggaag aggccagatt gatcattagc
    53101 gatcatctcg atctgttagc caatcacgac ttccgcactt taatgcgcgt cacgcgtctg
    53161 aaagaagatg tgctgaaaga agccgtcaat ctgatccagt cgctcgatcc gcgccccggg
    53221 cagtcgatcc agactggcga acctgagtat gtcattccag atgtgctggt gcgtaagcat
    53281 aacggtcact ggacggtaga actcaacagt gacagcattc cgcgtctgca aatcaaccag
    53341 cactacgcct cgatgtgcaa taacgcgcgc aacgatggtg acagccagtt tatccgcagc
    53401 aatctgcagg atgccaaatg gttgatcaag agtctggaaa gccgtaacga tacgctactg
    53461 cgcgtgagtc gctgtatcgt tgaacagcag caagccttct ttgagcaagg tgaagaatat
    53521 atgaaaccga tggtactggc cgatatcgcc caggctgtcg aaatgcatga atcgacgata
    53581 tctcgcgtga ccacgcaaaa atacctgcat agtccacgag gcatttttga actgaagtat
    53641 ttcttttcca gtcacgtcaa taccgagggc ggcggcgaag cttcctccac ggcgattcgt
    53701 gcactggtga agaaattaat cgcggcggaa aacccagcga aaccgttgag cgacagcaag
    53761 ttaacctctt tgctgtcgga acaaggtatc atggtggcac gccgcactgt tgcgaagtac
    53821 cgagagtctt tatccattcc gccgtcaaac cagcgtaaac agctcgtttg acccaaccga
    53881 taaggaagac actatgcagc tcaacattac cggaaataac gtcgagatca ccgaggcact
    53941 gcgcgaattt gttacagcta aatttgccaa acttgagcaa tattttgacc gaatcaacca
    54001 ggtctatgtt gttctgaaag tggagaaagt cacccacacc tcagatgcaa cactgcatgt
    54061 aaacggtggt gaaattcatg ccagcgcgga aggtcaggac atgtacgctg ccattgatgg
    54121 cttaattgat aagctggcac gccagttgac caaacataaa gataaactga aacaacacta
    54181 attgtccggg caattagcat gtgcatggcg gcctgttgtg cggcacaacg ggccatttgt
    54241 acggttaatg ctccgagcct gttccactgt ttgagtgggc aggttcttag gtgaaattat
    54301 gacaaataat gatacaactc tacagcttag cagtgttctt aacagggaat gtacgcgaag
    54361 ccgcgtccac tgtcagagca aaaaacgcgc cctggaaatc atcagcgagc tggcggcgaa
    54421 acaacttagc ctaccgcctc aggtggtttt tgaagctatc ctgacgcggg aaaaaatggg
    54481 cagtaccggt atcggcaatg gtattgccat tccgcatggc aaacttgaag aagatactct
    54541 gcgtgccgtt ggcgttttcg ttcagctcga aacgcctata gctttcgatg ccatcgacaa
    54601 ccaaccggtg gacttacttt ttgccctgct ggtgccggca gaccaaacta aaacgcacct
    54661 gcatactctg tcgctggtgg cgaaacgtct ggcggacaaa accatctgcc gccgtttacg
    54721 cgcagcccag agcgatgaag agctgtatca aatcattacg gataccgaag gtactccgga
    54781 tgaagcgtag ttattcggta atgtctcttt tagacgttgt gaggagaaac agtacatggt
    54841 actgatgatc gtcagcggac gttcaggttc aggtaaatct gtcgccctgc gtgcgctgga
    54901 agatatgggt ttttactgcg tggataacct tcccgtagtg ttgttacccg atctggctcg
    54961 aactctggcc gatcgcgaga tttctgccgc cgtcagcatt gatgttcgta atatgccgga
    55021 gtcaccagaa atattcgaac aggcgatgag taacctgcct gacgctttct caccgcaact
    55081 actgttcctg gatgccgacc gtaatacctt aattcgtcgt tacagtgaca cgcgccgact
    55141 gcatccgctt tccagcaaaa acctgtcgct ggaaagtgct atcgacaaag aaagcgattt
    55201 gctggagcct ctgcgttcgc gagcggatct gattgtcgat acctcagaaa tgtccgttca
    55261 cgagctggct gaaatgctgc gtacccgtct gctgggtaaa cgtgaacgcg aactgaccat
    55321 ggtctttgag tctttcggct tcaaacacgg tatccctatc gatgcagatt acgtctttga
    55381 cgtgcgcttc ttgccgaacc cgcactggga tccgaaactg cgtccaatga caggtcttga
    55441 taaacctgtc gccgcgttcc tcgaccgcca cacagaagta cacaatttta tctaccagac
    55501 gcgaagctat cttgagctat ggttacctat gctggaaacc aacaaccgta gctacctgac
    55561 ggtcgccatt ggttgtaccg gcgggaagca ccgttcggtg tatattgcag agcaactggc
    55621 agactacttc cgctcgcgcg gtaaaaacgt ccagtcacgc catcgtacgc tggaaaaacg
    55681 taaaccatga ccgtcaagca aactgttgaa atcacaaaca agctgggcat gcatgcccgg
    55741 cctgcaatga agctgtttga attaatgcag ggttttgacg ctgaagtgct gttacgtaat
    55801 gacgaaggca ccgaggctga agccaacagc gttattgcgc tgctgatgtt ggattctgcc
    55861 aaaggacggc agattgaagt tgaagcgacc ggtccacagg aagaggaagc actggccgcc
    55921 gttatcgccc tctttaattc tggttttgat gaagattaat cttcatcact ttgacataca
    55981 actcccttca agctcccccg acaataagaa aatcacgtac tgaaatcgtt ctcaatcaac
    56041 gtcatttgta cattttgtgc gcttttcact ttcagaagaa ccttaagaaa accttaagag
    56101 gcattgttta ggttttgttt aagttaatcg accatactgg agatcgtcag aaaatatttc
    56161 caggagatgg catgattcgc ttatctgaac aaagtcccct gggcaccgga agacatcgga
    56221 aatgttatgc gcatccggaa gatgcccaac gctgtatcaa gattgtctac catcgtggcg
    56281 atggtggcga taaagagatc cgccgtgagt taaagtacta cgcgcatctt ggtcgccggt
    56341 taaaagactg gagtggaata ccgcgctatc acggtaccgt tgagacggat tgcggaaccg
    56401 gttatgtcta cgatgtgatt gccgattttg acggcaagcc ttccattacg ctgaccgaat
    56461 ttgctgaaca atgccgttac gaagaagaca ttgcacaact gcgccagcta ctgaaacagc
    56521 tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc gctgaagccg cagaatatcc
    56581 tctgtcaccg catcagtgag tcagaggtta tcccggtcgt ctgcgacaac attggtgaaa
    56641 gcacattgat tccactggcg acctggtcga agtggtgttg tctgcgtaag caagagcggt
    56701 tatggaaacg atttatcgcc caaccggcgc tggctattgc cctgcagaaa gatctgcaac
    56761 ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc ttaatccagc tggtgttgtt
    56821 gcataaacgg ctcaccgcct aactgataca tctgccgtaa aatccacgcc tgacggctac
    56881 gcacgtagcc gtcaggcgtg gagactttga aacgaagtgg attaggtaat acagctgcca
    56941 gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt gtggaaataa cgttgtgccg
    57001 cagcttcgac gccaaacaca ccgtcgccaa attcggcgat attcaggtaa accgtcagga
    57061 tacgcttttt gctccagact gtttctatcc ccagcgttaa tccagcttcc agcccttttc
    57121 gtacccagct acgcccatcc cataaaaaga gatttttggc cgtctgctga gaaatcgttg
    57181 aagcaccgcg aatacggttt tcattgcgct cgttgtgcgc cagggctttc tcaatggaag
    57241 cgacatcaaa gccccagtgc tcaggaaatt tctgatcttc tgcggcaatc accgccagtc
    57301 ccatccacgg cgagatttga tccatactga cccagtcaga atgtgccacg taacgaaaat
    57361 tgccatgcag ccaggcgctg acctgtcgct cgaccattac cgctgagaag ggaacaggcg
    57421 caacgctaaa caacgcgatg cccccgcccc agaaaacggc gaggacaacc attaaccgca
    57481 aaagaaaacg gcgaacgaaa ctaaacaccg ttaagcggct tttactcatt cagccagaac
    57541 cagcacgcgg gaaaccagct tatcaatgcc gctcgccgct tctgcaatgt tctgcgccag
    57601 catatatgct ggggtggtga caatcttatt gtcttcatca accacgatat catcgacagg
    57661 acacggcaca tgctccgcgc ccatctcttc cagtacttct gcggtatcga tatcagtacc
    57721 gatggtcaaa cgcagcggga aatcgaaaat tttcggcagc atcgccgggg caatacacat
    57781 aaaaccaagc ggttttccgg cctgatgcat cgcttgtgcc agcgccttta attcacggtc
    57841 aacggtgcat tcgctaccaa gactggcaaa attgcttaaa ttcttcgccg cgccaacccc
    57901 ccccggcaca atcaacgcat ccagttcagc ggcatcggcc tgggccagag gacggatttc
    57961 accgcgcgtt attcgtgccg cttcaatcag cacattgcgc gtttccgtca tcgcttcgcc
    58021 agttaaatgg ttgataacat caacctgctg cttatccggt gcaaagcaga ccgcctgcgc
    58081 accgctgcgt gaaatagcta acaacgtcaa caccgcttca tgaatttcag aaccgtcata
    58141 gacgccgcat ccgctcagaa ttacgccaat tttcttcatt gtgatcatcc tttcgcaatt
    58201 gactgaaaca cattaatttt tttaataaaa atggtacgca tcacacattt aactgattca
    58261 tgtaacaaat catttaagtt ttgctatctt aactgcgtgc ggcctgaaaa acagtgctgt
    58321 gcccttgtaa ctcatcataa taatttacgg cgcagccaag atttccctgg tgttggcgca
    58381 gtattcgcgc accccggtct agccggggtc attttttagt ggcttttgcc acccacgctt
    58441 tcagcacttc tacgtcgtga cgccactctt ctttcatctc ttcaatccat tcaccgacgt
    58501 tatcttccca ggcgggaagg tcaggagact gaatttgctg acccagctgt tgcagatggc
    58561 gtaaccccac tgaccccgcc gcacctttaa ttttatgtcc ttcctcaaca atgccttttt
    58621 tatcctgcgc cgtcagattc gactccagca cgctgacata gcccggcatc atcttctcaa
    58681 acaccgctaa cccgtcggtg atcagcttcg gtcctacaag ttcgagatac tgttccagca
    58741 tgggaatatc gagcaatgct tctgatttac tgttctcttc tgtcgtcacc gtactctcct
    58801 catcatcctg ggtatcccag aatttcttga tcatcgcggt tagcgccgga accgaaagcg
    58861 gcttactcag cacatcatcc attccagcat tgaggtactc ttgtttgtct ttcagcacgt
    58921 tagcggttaa ggccaccagc ggcggtaaat cctcgcgcgg ataacgcttc gtcagttcac
    58981 gagagatatc cagcccggtc atatctggca actgaatatc cagcaacacc aggtcgtatt
    59041 cgcccggttt aaacatctcc agcgccgcct tgccggtcat ggcgacatca acgctgttac
    59101 ctaatttttc cagcacagaa cgcgcgacaa tcacgttcag ttcaatgtct tccaccagca
    59161 gcacattcag cgccggtaaa ggcatatcgt cttcatcaaa cgcatcatcg acctcttctg
    59221 ccaccgacgg tgcgtggatc gtcaacgtaa aggttgaacc tttgccctgt tcgctggtaa
    59281 ccgtaatatc gccgcccata tttttcgcca gacgatgaga aacggccaga ccaataccgg
    59341 tgccggtggc aggtttaccg ccatgactgt ctttcacctg gtaatacatg gcgaagattt
    59401 tatccagctc atcctgcgga atgccgatac cagaatcttc cacttcaaaa tgcagcatat
    59461 cgccttcatc gtagcgcacg cgcacggtaa cctggccttg ctgggtgaat ttgacggcgt
    59521 tactgatgag gttccacagg atctgccgta aacgcgtccc gtcggtaatg acctgatgcg
    59581 gtaatggcag cgtaggctcc aggttaaagc gcaatccttt ttgttgcgcc tgcaatgcgg
    59641 agagattttc cagatcggca aggaagctgg tgaaatcaac cggctgatta tcaagctgga
    59701 ccttgcgccg ttccatctta tccatgtcga taatatcgtt aaagatattc cccagcgtga
    59761 cggccgaaac atggatagtt ttgagatatt tttcctgctc ggcggtgagt tcggtatcca
    59821 gcagaatgcg gctcaggccg acgataccgt tcagcggtgt acgcaattcg tgactgatgg
    59881 tggaaataaa cgtcgttttg tcgcggctgg cgcgttcaag cgcatcctga taccgcttac
    59941 gctcggtaat gtcgcgacca aagcccatca aaccgtgacg tttacccacg cggtcgtagt
    60001 acggcacttt acggatttca aagcaggctt tgcgcccgtc cgggtaatcc agccactgtt
    60061 cataggtcag tgacacatta tgacggaaca ctttttcatc ggtttcaatg acttttgcgg
    60121 cggcttccgg tgagtaaaca tcagcaggtt tcagatgaac cagttgtttt tcgctttttc
    60181 cggtcagcag ctccatcgcg cggttacagc cggaaaactc tttatcttcg ttacgataaa
    60241 aaaccaggtc gggtgaagca tcaaggaagg aacgtaagaa tgaggattgc tgctcgagct
    60301 gaatttgtgt ctcttcgcgc tctttgattt caattttcag ttggccgaag gtttcctgca
    60361 gttctgcttc cgctttttca cgaacggcaa tttcctgatt tagctgggca atattatctt
    60421 ttaactgaac gttgaggctc aaatcgcgct cgcgcatctc ctccagtttt tgcaccagcc
    60481 gtgacagacg ttgtcgtgac tcctccagtt gctcgacgac caccgataga aagtagaccg
    60541 cccacggcgt aatcagcaaa ccaaagaaga tagaacgaat aacatcaatg ctttcgacct
    60601 gaccatgcag caccatggtt accgccattt gtaccacaat ggcaagaacg acgagggcca
    60661 gcgccagcaa cattgagaag cgcaccagac ctaacttcat catcaggtca acataatact
    60721 gcgccagcag acgaatttgc ttcattaggg aattccttca cgacaacctg ccccaataat
    60781 acccaattct gacagttacg ttataaattg tgtgagaaat gcggaattcc cctcaacgac
    60841 ctactccgtt ggaggtagcc acggacgtcc cagcgccgat ccctgtacgc catgttcatt
    60901 cagatagcga tccagctcga ccattcccgt ccatcgattt tcgcaccaca gcggagcaag
    60961 cagcgtcggg cgacgggcgc tggcggaaat gcggtggtag atcacttccg gtggcgtatg
    61021 gcgaatcatt tctccggcag tgagcgtata atcctccagt tcaataccgt tcaaacgccc
    61081 cgcttcccag gctttcgcca taatgctgcc tttcacaata tgcagcggat gcagctttat
    61141 gccgtccacg ctggtttcaa ccactcgctc cagcgtttgc aaacattcag cctgcccttc
    61201 gccaggcagg ccgacaatta agtgggaaca caccttcagc ccgcgctgac gtgccagctg
    61261 ggttgtacgc tgataacagg caaaatcatg gccgcggttg atgcgatgca gtgttttgtc
    61321 gtgggcggtt tgtagcccca gctccagcca cacttcgtaa ccctggtcct tatattcgca
    61381 aagcagatcc agcaccgcat ccggcacgca gtccgggcgg gtaccaacac acaaaccgac
    61441 aatattggcc tggctcaccg cctgctgata catagaacgc agcacctgaa cttccgcaaa
    61501 ggtgctggta tacgcctgaa agtaggccag atagcgttta gcgcggttaa ctaaattcgc
    61561 ctggtgcgcc agttgctcgg caatggaacg atgctgctgc gcttcatcgg caaacgaggc
    61621 aacattacag aatgtgcagc caccacgccc gatggtaccg tcacggttag ggcagctaaa
    61681 accgccgtgc agcgtcagct tatgcacctt ttgcccataa cgacgggtga gatcaccacc
    61741 aaacatattg actaatttct gtaactgcat aatctgatag accgcgcctt gaaaagaggc
    61801 caaagcctgc catttttagc tcaattcggc gatgacctag ctcaatcgtc ccggcctgct
    61861 tttatcaact gcataatcaa tcaaaattac cgaaatttca tgcataatca cataaatcac
    61921 ttttgcttat cttgtgtcag atttttttat ctccttatgg attttaggca aaaacagtag
    61981 catgaaacgt cattaccaat taaggcagta taaaatgctg gttttctcgt cagttcaagg
    62041 caggataagg gttaacacgc ctttatgaca gtcaggaatt gactgtttct ctaacgactc
    62101 tccttttagc cttaaagata aaatctattt taatttcagc catttaataa agaattttgc
    62161 gctaaagcac atttctgtac caataagctt gccatttgac ctgtatcagc tttcccgata
    62221 agttggaaat ccgctggaag ctttctggat gagcagcctg ctcatcatat ttatgcagta
    62281 attgagatcc cctcttcacc gtattaaccg atgcgaaaag gacaacaagg gggcgaatgc
    62341 gaggcgcgcg tatgacacgc aaaccccgtc gccacgctct ttctgtgccc gtgcgcagcg
    62401 gttcggaagt ggggttcccg cagagcctgg gggaggttca cgatatgttg tacgataaat
    62461 cccttgagag ggataactgt ggtttcggcc tgatcgccca catagaaggc gaacctagcc
    62521 acaaggtagt gcgtactgca atacacgcac tggcccgcat gcagcaccgt ggcgcgattc
    62581 tcgccgatgg taaaaccggc gacggttgcg gcttgctgtt acaaaaaccg gatcgctttt
    62641 ttcgcatcgt tgcgcaggag cgcggctggc gtttagcaaa aaactacgct gtcgggatgc
    62701 tcttcctgaa taaagatcct gaactcgccg ctgccgcacg ccgcatcgtt gaagaagaac
    62761 tgcaacgcga aaccttgtcg attgtgggct ggcgtgatgt ccccactaac gaaggcgtgc
    62821 tgggtgaaat cgccctctcc tctctgccac gcattgagca aatttttgtg aacgccccgg
    62881 caggctggcg tccacgcgat atggagcgcc gtctgtttat cgcccgccgc cgcattgaaa
    62941 agcgtctcga agccgacaaa gacttctacg tctgtagcct gtcgaatctg gtgaacatct
    63001 ataaaggtct gtgtatgccg gcggatctgc cgcgctttta tctggatctt gcggacctgc
    63061 gtctggaatc ggccatttgc ctgttccacc agcgcttctc cactaacacc gtaccgcgct
    63121 ggccgctggc gcaaccgttc cgctatctgg cgcataacgg tgaaatcaac accatcaccg
    63181 gtaaccgcca atgggcgcgt gcgcgtacct ataaattcca gacgccgctg atccctgacc
    63241 tgcacgacgc cgcgccattc gtcaacgaaa ccggttctga ctccagttcc atggataaca
    63301 tgctggaact gctgctggca ggcgggatgg atatcatccg cgccatgcgc ctgttagtgc
    63361 cacccgcctg gcagaacaac ccggatatgg acccggaact gcgtgccttc tttgacttta
    63421 actccatgca tatggagccg tgggatggtc cggcgggcat cgtgatgtcc gatggtcgct
    63481 tcgctgcctg taacctcgac cgtaacggtc tgcgtccggc acgctacgtc attaccaaag
    63541 acaagctcat cacctgcgcc tctgaagtcg gtatctggga ttaccagcct gacgaagtgg
    63601 tcgaaaaagg ccgcgtcggg ccaggcgaac tgatggtgat cgacactcgc agtgggcgta
    63661 ttctgcactc ggcagaaacc gatgacgatc tgaaaagccg ccacccttat aaagagtgga
    63721 tggagaaaaa cgtccgccga ctggtaccgt ttgaagatct gcccgatgaa gaagtgggta
    63781 gccgcgaact ggacgacgac acgcttgcca gctaccagaa acagtttaac tacagcgcgg
    63841 aagagctgga ctccgtaatt cgcgtactgg gcgaaaacgg tcaggaagcg gtcggttcga
    63901 tgggcgatga taccccattc gccgtgctct ccagtcagcc gcgcattatt tacgactact
    63961 tccgccagca gtttgcccag gtgactaacc cgccaatcga cccgctgcgt gaagcgcatg
    64021 ttatgtcgct cgccaccagt atcggtcgtg aaatgaacgt cttttgcgaa gcagagggcc
    64081 aggcgcaccg tttaagcttt aaatcgccga ttctgctcta ctccgatttc aaacagctca
    64141 cgacgatgaa agaggagcac taccgcgcag atacgctgga tatcaccttt gacgtcacta
    64201 aaaccacgct cgaagcgaca gtcaaagagc tatgcgacaa agccgaaaaa atggtacgta
    64261 gcggcaccgt gttgctggtg ctctccgacc ggaatatcgc taaagatcgc ctgccggttc
    64321 cggctccgat ggcggttggc gcgatccaga cccgtctggt cgatcaaagc ctgcgttgcg
    64381 atgccaacat catcgtcgaa accgccagcg cccgcgatcc gcaccacttc gccgtgttgc
    64441 tgggtttcgg cgcgacggct atttatccgt accttgccta tgaaacgctg ggccgcctgg
    64501 tagacaccca cgcgattgcc aaagattatc gtaccgtgat gctcaactac cgtaacggca
    64561 tcaacaaagg cttgtacaaa atcatgtcca aaatgggcat ctccaccatc gcctcttacc
    64621 gctgctcgaa actgtttgaa gcggtcggtc tacacgatga tgtagtgggc ctgtgcttcc
    64681 agggcgcggt cagccgcatt ggtggggcaa gctttgaaga cttccagcag gatctgctga
    64741 atctgtcgaa acgtgcctgg ctggcgcgca agccgattag ccagggcggc ctgctgaaat
    64801 acgtccacgg cggcgaatac cacgcctaca acccggacgt ggtgcgcacg ctgcaacagg
    64861 cggtacaaag cggcgagtac agcgactatc aggaatacgc gaagctggtt aatgagcgtc
    64921 cggcaaccac gctgcgcgat ctgctggcaa ttacgccggg tgaaaacgcg gtcaacattg
    64981 ctgatgttga accggcaagc gaactgttta aacgctttga taccgccgcg atgtctatcg
    65041 gcgcgttaag cccggaagcc cacgaggcgc tggcggaagc gatgaatagc atcggcggta
    65101 actcgaactc cggtgaaggc ggcgaagacc cggcgcgcta tggcactaac aaagtgtcgc
    65161 gcatcaagca ggtggcttct ggtcgcttcg gcgttactcc ggcgtatctg gtcaatgccg
    65221 acgtcattca gattaaagtc gcccagggcg cgaagccggg cgaaggcggt cagttgccgg
    65281 gcgacaaagt cactccttac atcgccaaac tgcgctattc ggtgcccgga gtgacgctga
    65341 tctccccgcc gccgcaccac gatatctact ctatcgaaga cttagcgcag cttattttcg
    65401 acctcaagca ggttaacccg aaagcgatga tctccgttaa gctggtgtcc gaaccaggcg
    65461 ttggcaccat cgcgactggc gtggcgaaag cttatgcgga cttgatcacc atcgcgggct
    65521 atgacggcgg caccggcgca agcccgctgt cgtcagtgaa atatgcaggc tgcccgtggg
    65581 aactcggtct ggtggaaacc caacaggcgc tggttgccaa cggcttgcgt cataaaattc
    65641 gtttgcaggt tgatggcggc ctgaaaacgg gtgtcgatat catcaaagcg gcgattctcg
    65701 gcgcagaaag cttcggcttc ggcactggcc caatggtggc gctcggctgt aaatatctgc
    65761 gtatttgcca cctgaacaac tgcgccacgg gtgtagcaac tcaggatgac aaactgcgta
    65821 agaatcacta tcacggcctg ccgttcaagg tgactaatta ctttgagttt atcgcccgtg
    65881 aaacccgcga gctgatggca cagctgggag taacgcgtct ggtggatctg attggtcgca
    65941 ccgacctgct gaaagagctg gacggtttca ccgccaaaca gcagaaactg gcgctgtcga
    66001 agctgctgga gactgccgag ccacatgcag gtaagtcact ctactgcact gaaaacaacc
    66061 cgccgtttga taacggccta ctgaacgcgc agttgctgca acaggcgaaa ccgtttgtcg
    66121 atgaacgcca gagcaaaacc ttctggttcg atatccgcaa caccgaccgt tctgtcggcg
    66181 cgtcgctttc aggctatatc gcccagacgc acggcgatca ggggctggca gccgatccta
    66241 tcaaagcgta cttcaacggc accgcaggcc agagtttcgg tgtgtggaac gcgggcggcg
    66301 tggaactgta cctgaccggg gatgccaacg actatgtcgg taaaggcatg gcgggcggct
    66361 taatcgccat tcatcctccg gttggttccg ccttccgcag ccatgaagca agcattatcg
    66421 gcaacacctg cctgtatggc gcgaccggtg gtcgtctgta tgccgcaggc cgcgcgggtg
    66481 aacgtttcgg cgtgcggaac tccggtgcta tcaccgtggt agaaggcatt ggcgacaacg
    66541 gctgtgaata tatgacgggc ggtattgtct gtattctggg taaaaccggc gttaacttcg
    66601 gcgcgggcat gaccggcggc ttcgcttacg ttctcgatga aagcggtgat ttccgcaaac
    66661 gcgttaaccc ggaactggtc gaagtcttaa gcgtcgacga tctggcgatc catgaagagc
    66721 atctgcgcgg tcttatcacc gagcatgttc agcataccgg ttctcagcgc ggtgaagaga
    66781 ttctggcgaa ctggtcaacc ttcgccacga aattcgcgct ggttaaaccg aagtccagtg
    66841 atgtaaaagc actgctgggt caccgtagtc gtagcgcagc agagttgcgc gtgcaggcgc
    66901 agtaaggggt agcaacaatg agtcagaatg tttatcaatt tatcgacctg cagcgcgttg
    66961 atccgccaaa gaaaccgctg aagatccgta aaattgagtt tgttgaaatt tacgagccgt
    67021 tttccgaagg ccaggccaaa gcgcaggctg accgctgtct gtcgtgcggc aacccatact
    67081 gcgagtggaa atgcccggta cacaactaca tcccaaactg gctgaagctc gccaacgagg
    67141 ggcgtatttt tgaagcggca gaactgtcgc atcagaccaa cacactgccg gaagtatgcg
    67201 gacgtgtctg cccgcaagac cgtctgtgcg aaggttcctg caccctgaac gatgagttcg
    67261 gtgcggtgac catcggcaac attgagcgtt atatcaatga taaagcgttc gaaatgggct
    67321 ggcgtccgga tatgtccggc gtgaagcaaa ccggtaaaaa agtggcgatt atcggtgcag
    67381 gcccggcagg tctggcgtgt gcggatgtcc tgacgcgtaa cggcgtaaaa gccgttgtct
    67441 tcgaccgtca tccagaaatt ggcgggctgc tgaccttcgg tattccggcc ttcaagctgg
    67501 aaaaagaggt aatgacgcgt cgccgtgaaa tcttcaccgg catgggtatt gaattcaaac
    67561 tcaataccga agtgggccgc gacgtacagc tggacgatct gctgagtgat tacgatgccg
    67621 tgttccttgg cgttgggact tatcagtcaa tgcgcggcgg gctggaaaac gaagacgccg
    67681 atggcgtgta cgcagcgctg ccgttcctta tcgccaacac caaacagtta atgggctttg
    67741 gtgaaacccg cgacgaaccg ttcgtcagca tggaaggcaa acgcgtggta gtacttggcg
    67801 gtggcgacac tgcgatggac tgcgtgcgca cgtctgtacg ccagggggca aagcacgtta
    67861 cctgtgccta tcgtcgtgat gaagagaaca tgccgggttc ccgccgcgaa gtgaaaaacg
    67921 cgcgggaaga aggcgtagag ttcaaattca acgtccagcc actggggatt gaagtgaacg
    67981 gtaacggcaa agtcagcggc gtaaaaatgg tacgtaccga aatgggcgaa ccggatgcca
    68041 aaggccgtcg ccgcgcggag atcgttgcag gttccgaaca tatcgttccg gcagatgcgg
    68101 tgatcatggc gtttggtttc cgtccacaca acatggaatg gctggcaaaa cacagcgtcg
    68161 agctggattc acaaggccgc atcatcgctc cggaaggcaa cgacaacgct ttccagacca
    68221 gcaacccgaa aatctttgct ggcggcgata tcgtccgtgg ttccgatctg gtcgttaccg
    68281 ccatcgccga aggtcgcaaa gcggcagacg gcattatgaa ctggctggaa gtttaagctc
    68341 tgtcccagcg ccgcctcctc agaaaagagt cggcgctgaa attctccttt ctttctggaa
    68401 atcgcctcgc ataatctgaa gaaaaccaat gtaatacgct catctggatt gttaccggta
    68461 gatgcccggc gtatagctga ttccattatt caacgcagcg ccatataaag ggacagataa
    68521 tggaatcgct ctcagagaga acctcaacag gctaccagca aatccacgac ggcattattc
    68581 atctggtcga tagcgcccga acggaaacgg tacgtagcgt taacgcgctg atgaccgcga
    68641 cgtactggga aattggccga cggattgtcg aatttgaaca aggtggtgag gccagagcag
    68701 cttatggtgc acagttgatt gatcggctat cgaaggattt aagtcgacgg tataaacgtg
    68761 gggtctctgc aaaaaactta cgccaaatga ggctttttta cctctttttt caacatgttg
    68821 aaattcgcca gacagtgtct ggcgaattaa caccattggg aattccgcag acaccatctg
    68881 cagaattccc atcggcaaag atttggcaga cgctgtctgc caaattgaga ggctgtttga
    68941 tatttccaca gcttttgtat tgcatctcct attttcggtt tgtatattca tgcaaataaa
    69001 cgaaggaaat gaggtagcct gagtttaacg gacactcctt cctgaaatag aatggcatca
    69061 gaaggagcta ataatgagca gaaaaaccca acgttactct aaagagttca aagccgaagc
    69121 tgtcagaacg gttcttgaaa atcaactttc gatcagtgaa ggcgcttccc gattatctct
    69181 tcctgaaggc actttaggac aatgggttac cgcacggtgg ctgaactgga atctgaaatt
    69241 ctgcaactgc gtaaggcgtt aaatgaagct cgccttgagc gagatatat
//
LOCUS       DAETPZ010000007        68193 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.007, whole genome shotgun sequence.
ACCESSION   DAETPZ010000007 DAETPZ010000000
VERSION     DAETPZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 68193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.007"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..247
                     /locus_tag="K0W59_000562"
     CDS             <1..247
                     /locus_tag="K0W59_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBI3021723.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..1052)
                     /gene="deoR"
                     /locus_tag="K0W59_000563"
     CDS             complement(294..1052)
                     /gene="deoR"
                     /locus_tag="K0W59_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HBI3021724.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="K0W59_000564"
     CDS             complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="K0W59_000564"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBI3021725.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1991..3217
                     /gene="mdfA"
                     /locus_tag="K0W59_000565"
     CDS             1991..3217
                     /gene="mdfA"
                     /locus_tag="K0W59_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HBI3021726.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTMLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMVNVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3258..3542)
                     /locus_tag="K0W59_000566"
     CDS             complement(3258..3542)
                     /locus_tag="K0W59_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3021727.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="K0W59_000567"
     CDS             complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="K0W59_000567"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HBI3021728.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMKNAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4443..5651)
                     /locus_tag="K0W59_000568"
     CDS             complement(4443..5651)
                     /locus_tag="K0W59_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3021729.1"
                     /translation="MTVNSSRNALKRRTRALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAAAKPD
                     TKTQTAMENS"
     gene            5735..6271
                     /gene="rcdA"
                     /locus_tag="K0W59_000569"
     CDS             5735..6271
                     /gene="rcdA"
                     /locus_tag="K0W59_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HBI3021730.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6446..8131)
                     /locus_tag="K0W59_000570"
     CDS             complement(6446..8131)
                     /locus_tag="K0W59_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HBI3021731.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAITNVLLTLAGTIIVMVWPGLG"
     gene            8401..8778
                     /locus_tag="K0W59_000571"
     CDS             8401..8778
                     /locus_tag="K0W59_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HBI3021732.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="K0W59_000572"
     CDS             complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="K0W59_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HBI3021733.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9225..9512
                     /locus_tag="K0W59_000573"
     CDS             9225..9512
                     /locus_tag="K0W59_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HBI3021734.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVVKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9496..10218
                     /gene="nfsA"
                     /locus_tag="K0W59_000574"
     CDS             9496..10218
                     /gene="nfsA"
                     /locus_tag="K0W59_000574"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HBI3021735.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10279..11181
                     /gene="rimK"
                     /locus_tag="K0W59_000575"
     CDS             10279..11181
                     /gene="rimK"
                     /locus_tag="K0W59_000575"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HBI3021736.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11269..11745
                     /locus_tag="K0W59_000576"
     CDS             11269..11745
                     /locus_tag="K0W59_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HBI3021737.1"
                     /translation="MTSLVVPGLDTLRQWLDGLGMSFFECNNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12097..13209
                     /gene="potF"
                     /locus_tag="K0W59_000577"
     CDS             12097..13209
                     /gene="potF"
                     /locus_tag="K0W59_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HBI3021738.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRVKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13304..14380
                     /gene="potG"
                     /locus_tag="K0W59_000578"
     CDS             13304..14380
                     /gene="potG"
                     /locus_tag="K0W59_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HBI3021739.1"
                     /translation="MNDAIPRPQAKTRKVLTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPT"
     gene            14447..15400
                     /gene="potH"
                     /locus_tag="K0W59_000579"
     CDS             14447..15400
                     /gene="potH"
                     /locus_tag="K0W59_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HBI3021740.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15397..16242
                     /gene="potI"
                     /locus_tag="K0W59_000580"
     CDS             15397..16242
                     /gene="potI"
                     /locus_tag="K0W59_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HBI3021741.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16302..16790
                     /locus_tag="K0W59_000581"
     CDS             16302..16790
                     /locus_tag="K0W59_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HBI3021742.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16831..17958
                     /gene="rlmC"
                     /locus_tag="K0W59_000582"
     CDS             16831..17958
                     /gene="rlmC"
                     /locus_tag="K0W59_000582"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HBI3021743.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="K0W59_000583"
     CDS             complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="K0W59_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HBI3021744.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19156..19824)
                     /gene="artM"
                     /locus_tag="K0W59_000584"
     CDS             complement(19156..19824)
                     /gene="artM"
                     /locus_tag="K0W59_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HBI3021745.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="K0W59_000585"
     CDS             complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="K0W59_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HBI3021746.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="K0W59_000586"
     CDS             complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="K0W59_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI3021747.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21296..22024)
                     /gene="artP"
                     /locus_tag="K0W59_000587"
     CDS             complement(21296..22024)
                     /gene="artP"
                     /locus_tag="K0W59_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HBI3021748.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22242..22757)
                     /locus_tag="K0W59_000588"
     CDS             complement(22242..22757)
                     /locus_tag="K0W59_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBI3021749.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23406..25175
                     /locus_tag="K0W59_000589"
     CDS             23406..25175
                     /locus_tag="K0W59_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBI3021750.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNAFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSIGARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25386..25709
                     /locus_tag="K0W59_000590"
     CDS             25386..25709
                     /locus_tag="K0W59_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBI3021751.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25706..26536
                     /gene="amiD"
                     /locus_tag="K0W59_000591"
     CDS             25706..26536
                     /gene="amiD"
                     /locus_tag="K0W59_000591"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HBI3021752.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26533..27546)
                     /locus_tag="K0W59_000592"
     CDS             complement(26533..27546)
                     /locus_tag="K0W59_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBI3021753.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIAHSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27645..29075)
                     /locus_tag="K0W59_000593"
     CDS             complement(27645..29075)
                     /locus_tag="K0W59_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HBI3021754.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="K0W59_000594"
     CDS             complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="K0W59_000594"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBI3021755.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGAHIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="K0W59_000595"
     CDS             complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="K0W59_000595"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HBI3021756.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="K0W59_000596"
     CDS             complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="K0W59_000596"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HBI3021757.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPNLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="K0W59_000597"
                     /gene_synonym="priS"
     CDS             complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="K0W59_000597"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBI3021758.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPSFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="K0W59_000598"
     CDS             complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="K0W59_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HBI3021759.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="K0W59_000599"
     CDS             complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="K0W59_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HBI3021760.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYAEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            37227..38885
                     /locus_tag="K0W59_000600"
     CDS             37227..38885
                     /locus_tag="K0W59_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HBI3021761.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(38882..39838)
                     /locus_tag="K0W59_000601"
     CDS             complement(38882..39838)
                     /locus_tag="K0W59_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HBI3021762.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            39989..41104
                     /gene="macA"
                     /locus_tag="K0W59_000602"
     CDS             39989..41104
                     /gene="macA"
                     /locus_tag="K0W59_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HBI3021763.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMGGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            41101..43047
                     /gene="macB"
                     /locus_tag="K0W59_000603"
     CDS             41101..43047
                     /gene="macB"
                     /locus_tag="K0W59_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HBI3021764.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(43120..43344)
                     /gene="cspD"
                     /locus_tag="K0W59_000604"
     CDS             complement(43120..43344)
                     /gene="cspD"
                     /locus_tag="K0W59_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HBI3021765.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            43667..43987
                     /gene="clpS"
                     /locus_tag="K0W59_000605"
     CDS             43667..43987
                     /gene="clpS"
                     /locus_tag="K0W59_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HBI3021766.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            44018..46294
                     /gene="clpA"
                     /locus_tag="K0W59_000606"
     CDS             44018..46294
                     /gene="clpA"
                     /locus_tag="K0W59_000606"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HBI3021767.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(46697..46784)
                     /locus_tag="K0W59_000607"
     tRNA            complement(46697..46784)
                     /locus_tag="K0W59_000607"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46748..46750),aa:Ser,seq:gga)
     gene            complement(47038..47256)
                     /gene="infA"
                     /locus_tag="K0W59_000608"
     CDS             complement(47038..47256)
                     /gene="infA"
                     /locus_tag="K0W59_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBI3021768.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(47541..48245)
                     /gene="aat"
                     /locus_tag="K0W59_000609"
     CDS             complement(47541..48245)
                     /gene="aat"
                     /locus_tag="K0W59_000609"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HBI3021769.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(48287..50008)
                     /gene="cydC"
                     /locus_tag="K0W59_000610"
     CDS             complement(48287..50008)
                     /gene="cydC"
                     /locus_tag="K0W59_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HBI3021770.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(50009..51775)
                     /gene="cydD"
                     /locus_tag="K0W59_000611"
     CDS             complement(50009..51775)
                     /gene="cydD"
                     /locus_tag="K0W59_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HBI3021771.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(51898..52863)
                     /gene="trxB"
                     /locus_tag="K0W59_000612"
     CDS             complement(51898..52863)
                     /gene="trxB"
                     /locus_tag="K0W59_000612"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBI3021772.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            53408..53902
                     /gene="lrp"
                     /locus_tag="K0W59_000613"
     CDS             53408..53902
                     /gene="lrp"
                     /locus_tag="K0W59_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HBI3021773.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            54037..58143
                     /gene="ftsK"
                     /locus_tag="K0W59_000614"
     CDS             54037..58143
                     /gene="ftsK"
                     /locus_tag="K0W59_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBI3021774.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAVDPDDVLFLGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSIFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFTQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            58298..58909
                     /gene="lolA"
                     /locus_tag="K0W59_000615"
     CDS             58298..58909
                     /gene="lolA"
                     /locus_tag="K0W59_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HBI3021775.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDEQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            58920..60263
                     /gene="rarA"
                     /locus_tag="K0W59_000616"
                     /gene_synonym="mgsA"
     CDS             58920..60263
                     /gene="rarA"
                     /locus_tag="K0W59_000616"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HBI3021776.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            60354..61646
                     /gene="serS"
                     /locus_tag="K0W59_000617"
     CDS             60354..61646
                     /gene="serS"
                     /locus_tag="K0W59_000617"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBI3021777.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            61886..64330
                     /gene="dmsA"
                     /locus_tag="K0W59_000618"
                     /pseudo
     CDS             61886..64330
                     /gene="dmsA"
                     /locus_tag="K0W59_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            64341..64958
                     /gene="dmsB"
                     /locus_tag="K0W59_000619"
     CDS             64341..64958
                     /gene="dmsB"
                     /locus_tag="K0W59_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBI3021778.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            64960..65823
                     /gene="dmsC"
                     /locus_tag="K0W59_000620"
     CDS             64960..65823
                     /gene="dmsC"
                     /locus_tag="K0W59_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HBI3021779.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(65859..66485)
                     /locus_tag="K0W59_000621"
     CDS             complement(65859..66485)
                     /locus_tag="K0W59_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBI3021780.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            66800..67948
                     /locus_tag="K0W59_000622"
     CDS             66800..67948
                     /locus_tag="K0W59_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3021781.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGSVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT        16938 a        16866 c        17864 g        16525 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      481 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      541 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      601 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      661 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      721 atcgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      781 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      841 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      901 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      961 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
     1021 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     1081 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     1141 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1201 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1261 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1321 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1381 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1441 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1501 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1561 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1621 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1681 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1741 atacaggcaa gtcgttgaga ataaagtgca ggttacactg ggtaaagcgg catcgtctta
     1801 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1861 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1921 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1981 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     2041 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     2101 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     2161 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2221 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct
     2281 ggcaatatta ctggcgcaaa acattgaaca attcaccatg ttgcgcttct tgcagggcat
     2341 aagcctctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
     2401 ggtttgtatc aagatcaccg cgctgatggt gaacgtggcg ctgattgctc cgctacttgg
     2461 tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt
     2521 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccac
     2581 gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa
     2641 gaacggccgc tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat
     2701 cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt
     2761 gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac
     2821 ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct
     2881 gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg
     2941 gttaagtatt tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct
     3001 gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct
     3061 gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact
     3121 gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt
     3181 aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc
     3241 gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct
     3301 tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca
     3361 ggagccacat agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact
     3421 gaatcaggaa ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc
     3481 atcgccacac cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc
     3541 atttcgcctg aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc
     3601 attgtgttgc cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac
     3661 tttatcgatc acatccagta cgccttcacg gttattggag cctgcccggt attttgccgc
     3721 tgcgacgacc gcgctgccgg catttttcat tgcaaaacta aagcctgcct gacgcagcat
     3781 ctcaatatcg ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa
     3841 tttctgcagt tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct
     3901 gccgttgccg gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg
     3961 tacttgtgga atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa
     4021 gttgtcaaag ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat
     4081 ggcatcgtca tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac
     4141 ttccgggcgc gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc
     4201 attaaaaaca tctttgcctt cactcactac ccagccgccg ttttcagcca caaaggcaat
     4261 ttcattcgca atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac
     4321 cacaaagcga atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt
     4381 ataggttttt tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatact
     4441 catcaactat tctccatcgc cgtctgcgtt ttggtatcgg gtttggcggc ggctttcgcg
     4501 acaatagccg cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa
     4561 tgttcgccga gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt
     4621 gctaccacac tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc
     4681 agcgggaagc ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag
     4741 gcgctatcga caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga
     4801 accacggcaa cgcgactgta acggtcgatg aaccaaccgc cggtgaagcg tccaacggtc
     4861 atccccaggg taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca
     4921 tcaaccatta ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc catcgccagc
     4981 actacaacac caatcaacag caactggata tcgcgataaa aaggaatgcc tttttcgcca
     5041 tgctgggtgc catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata
     5101 taaataggcg cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca
     5161 aaggccgtca gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg
     5221 tgcatcatcg gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt
     5281 atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc
     5341 aggggcgatg tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac
     5401 atcgtgacca ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga
     5461 ataccgctca tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag
     5521 acagagagaa tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct
     5581 ggcaaaaaga agaacataaa cagcgccctg gttcggcgtt tcaatgcatt acgtgaagaa
     5641 tttacggtca tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt
     5701 acatttgtac acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg
     5761 gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt
     5821 tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt
     5881 ttcaggaatt gatgagttgt tgctggaagc gttcagcagt tttactgaga ttatgtcccg
     5941 gcaatatcag gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac
     6001 cgatatgatc tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct
     6061 ctacgcgctg gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg
     6121 cagtcagcaa acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt
     6181 tattgagggg atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat
     6241 tttgaggatg gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca
     6301 tcaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa
     6361 agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa
     6421 tttcattttc agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg
     6481 tccctgccag cgtcagcagg acgttggtga ttgcgtaggt gcccgcatag cccagcgcag
     6541 ggatgttact gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc
     6601 ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga
     6661 aacagataac cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa
     6721 tcgcgcccag gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca
     6781 ccatcaagcc gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg
     6841 ggtggttagc acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc
     6901 ccatgccgaa actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa
     6961 caaagaaggc gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga
     7021 tgcgatcggc gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt
     7081 tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa
     7141 ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt
     7201 ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt
     7261 ctttaccgtt acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg
     7321 ctatttcatc gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac
     7381 gtcgaatacg ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt
     7441 tgccgtcggt ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat
     7501 aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg
     7561 tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca
     7621 gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct
     7681 gcctgctttc catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg
     7741 acgtcataga gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac
     7801 ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat
     7861 ttttcccatc gcgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga
     7921 aaataaacag cataaagcca agattaagcg catcggtgtt aatgctgaaa tgttgttggc
     7981 ctaataacag cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc
     8041 gtaactttcc gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc
     8101 catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc
     8161 tgttgaaaga aatggtaatt tacgataatg tgttttacca gaattcaggg tgcagattca
     8221 ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg
     8281 tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat
     8341 cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga
     8401 gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg
     8461 tgcctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc
     8521 ggcttgctgt ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa
     8581 gtcctgaaac ctctatttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg
     8641 ctgttttttt caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg
     8701 ttctggtgtg cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag
     8761 cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga
     8821 ttttctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa
     8881 atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc
     8941 ccttccgcac gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc
     9001 tctgccagat cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt
     9061 tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct
     9121 gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca
     9181 gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta
     9241 aattgcctaa aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc
     9301 tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga
     9361 ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc
     9421 aggtggtaaa acgtcttgcc ccacagctga tgaaccgtcc accgcaatat tcacgttcag
     9481 aaagagaaaa agataatgac gccaaccatt gaacttattt gtggccatcg ctccattcgc
     9541 catttcactg atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt
     9601 gcgacgtcca gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca
     9661 ttacgtgaag aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag
     9721 ttctgggtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc
     9781 ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca
     9841 ttaaccgcag cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat
     9901 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     9961 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
    10021 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
    10081 ctggcggaat attacctcac ccgtggcagc aataatcgct gggatacctg gagcgatcat
    10141 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    10201 ggttgggcga cgcgctaaaa ccgccacgtc gatgtatgat acgcgggctt ttgaccaggt
    10261 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    10321 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    10381 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta ttcactacaa aggccgcaag
    10441 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    10501 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    10561 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    10621 cctgttacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    10681 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    10741 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    10801 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
    10861 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
    10921 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    10981 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    11041 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    11101 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    11161 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    11221 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    11281 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg gcctggggat gagttttttt
    11341 gaatgtaata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    11401 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    11461 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    11521 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    11581 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    11641 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    11701 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    11761 acgcagtcgc tgccgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    11821 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
    11881 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    11941 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    12001 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    12061 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
    12121 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    12181 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    12241 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    12301 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
    12361 cttgaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag
    12421 tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa
    12481 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
    12541 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat
    12601 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
    12661 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    12721 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    12781 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    12841 tggcaggcgt caaaccgcgt gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    12901 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgcctgcgga tgccaaaaac
    12961 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    13021 tccgaccatg tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    13081 gtccgtgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    13141 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    13201 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    13261 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    13321 tcgcccgcag gcgaaaaccc gtaaggtgct gacgccgcta ttagaaatcc gcaacctcac
    13381 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    13441 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc
    13501 aggtttcgaa caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt
    13561 tccgccttac ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccgcatat
    13621 gaccgtggaa cagaacatcg cttttggcct gaaacaggac aaactaccga aagcggaaat
    13681 ttccagtcgg gtcaatgaga tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa
    13741 accgcatcag ctttctggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa
    13801 gcgcccgaaa ctattactgc tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga
    13861 ccggatgcag cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt
    13921 cacccacgat caggaagagg cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg
    13981 gaaatttgtc cagattggcg aaccggaaga gatctacgag catccgacta cccgctatag
    14041 cgccgaattt attggctcgg taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga
    14101 tggcctggtg cttgattcgc cggggctggt gcatccactg aaagtcgacg cggatgcctc
    14161 ggtggtcgat aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga
    14221 agagccgccc gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct
    14281 cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gcgcccagct
    14341 acaaaacgcc catcgtcatc gtaaagggtt accgacctag ggcgacgaag tgcgtttgtg
    14401 ctgggaagtg gacagcagtg tggtgctgac ggtttaagga gcaaagatga atacacttga
    14461 acctgctgcc cagtcgaaac cgccgggcgg atttaagctg tggttgtcgc agctgcaaat
    14521 gaagcatggg cgcaaactgg tcattgcgtt gccatatatc tggttgatct tgctgtttct
    14581 gctgccattt ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc
    14641 ttataccgaa ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa
    14701 ttttctgcaa ctgaccgacg atccgctcta tttcgatgct tatctccagt cgctacaggt
    14761 ggctgcgatt tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc
    14821 gcacagtaag ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac
    14881 ctcgtttctg atccgcgttt atgcctggat gggaatatta aaaaacaacg gtgtgctgaa
    14941 taattttctg ctgtggctgg gggttatcga tcaaccgctg accattcttc ataccaatct
    15001 ggcagtttat attggcattg tttacgccta cgtgccgttt atggtgctgc cgatttatac
    15061 cgcgttgatt cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc
    15121 gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc
    15181 gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc
    15241 agacagcatc atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc
    15301 ggtggcctcg gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt
    15361 ccacaaacac cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt
    15421 tcgccctggc ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg
    15481 atgctggtta tctattcgtt taacagctcg aagctggtga cggtgtgggc cggctggtca
    15541 acgcgctggt atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggttcaagc
    15601 ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg
    15661 gtgctggtgc gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg
    15721 ccgctggtaa tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt
    15781 gctcatgcca ttggctggcc tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc
    15841 actttctgta cggcttatgt ggcggtcgtt atttcgtcgc gtctgcggga actggatcgc
    15901 tcgatagaag aagccgcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt
    15961 acgctaccga tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg
    16021 cttgatgatc tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg
    16081 ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct ggcaacattg
    16141 atcctcggcg cggtcggaat tgtcggattt atcgcctggt atctgatggc gcgcgcagaa
    16201 aaacagcgga tacgcgacat ccaacgtgca agacgtggct gaagacacta aaatttgcca
    16261 acctggctac ataatgccgc gcatgtcgcg gcattgtttt catggaagac gaaacgttgg
    16321 gattttttaa gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg
    16381 tggcggcgct cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc
    16441 tgggcgtgcg cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc
    16501 tggtcttttt aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg
    16561 tgaaagggcg tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt
    16621 acttgtgggc ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat
    16681 caaaacgtga aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga
    16741 tgctgctgtt cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat
    16801 cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc
    16861 tgtcgttcct gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc
    16921 gatcttaaaa atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc
    16981 ccggaacaag ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca
    17041 ctgctcggta tgctgcatcg cgatggcacg ccggaagacc tttgtgactg cccgctttat
    17101 ccagcctcat ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta
    17161 acgccctaca acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc
    17221 cagagtgatg gcggcatgat gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa
    17281 ctgcgtaagg cgctgccgtg gttacacgaa caactaccgc agctgaaagt tattaccgtc
    17341 aatattcagc cggtacatat ggcgattatg gaaggggaga cggagatcta cctgaccgaa
    17401 caacaggcac tggcggagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc
    17461 ttccagacta atccggcggt cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga
    17521 cagctgccgg ttaaacatat gtgggatctg ttctgcggtg tggggggctt tggtttacac
    17581 tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg cgccagaggc cattgcctgt
    17641 gcaaagcagt cagccgctga actgggctta actcgtttac aatttcaggc gctggactcc
    17701 actcagtttg ccactgctca gggggaggtg ccggagctgg tgctggttaa cccgccgcgc
    17761 cgcggcattg gtaaaccgct gtgtgattat ctttcaacga tggcaccgcg ttttattatc
    17821 tactccagct gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc
    17881 atcgaacggg tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgttgacg
    17941 ctgctggtga agcaataaaa aagccacagg tgcggcttca gattgctgat caggtgcgct
    18001 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaatcat gctaaatcaa
    18061 tacattgcag agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt
    18121 catcagtctc aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc
    18181 tttaattgct gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac
    18241 agcgataccc aggccggtgc cgaagtattg cggatcggtc actttttcag tcgcaacgcc
    18301 cagttgcgga ttggttttca gccattcgtt taccaccgcg gtgtcaccaa ataccccatc
    18361 aatacgacca tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt
    18421 cacttccggg tgctgatcct gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat
    18481 gcgtttgcct ttcaggtcgg caaacgtttt gtaggtatct tttttggcaa tcacgacggc
    18541 tgagttttca tagtagggtg tggtaaacga aacctgcttg ctacgctccg gggtgatatc
    18601 cataccggag attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa
    18661 cgcgtgatta gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc
    18721 gatatcaaag ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc
    18781 tgaaacgcca aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa
    18841 agcggcaaga actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg
    18901 ccgtgtctgc ggcatgggat aacaatgcca ccaagtgaat ttatatgcaa taaacatgat
    18961 taaacaattt aaatgaaata aaaaaagacg gataacttag tgagttgtcc gtcttcatta
    19021 taagaattta tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac
    19081 cgtgctgatg tctgatgcga cgctggcgcg tcttatcaga cctacaaacc cccccggcga
    19141 atgtacgcag ctacattaat tccgccgttc gaatgccagt gctttgcgct cgatcagacg
    19201 catcatcagc gtcagcaggc cgttgacgac caggtaaata atccctgccg caccgaacac
    19261 cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt
    19321 aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga
    19381 gagcgagcgt ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc
    19441 cagggcgcta caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag
    19501 ctgcgtggta tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc
    19561 tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc ggaaactggc ccggcccgta
    19621 ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg
    19681 caccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag
    19741 aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat
    19801 cagctcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg
    19861 tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc
    19921 acaatgtacc aggtaaatgg ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt
    19981 aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg
    20041 ttaccgaggc caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata
    20101 gccgatttcg acagccccag cgcctgacca gattcccact ggcccaccgg tacagctttc
    20161 aacgcgccgc gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca
    20221 ccacaaagga acggactcac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg
    20281 aacccaagat tgatagtgaa gccatccgaa agcgtcagca gtaactgcga ggagccaaaa
    20341 tagataaaca gcactaccag aatttctggc aggccacgca gaatggttac cagcgctgaa
    20401 cctgcccacg cgacaggacg ccatttcgct gactcccata ccgcaaagaa catcgccagc
    20461 gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct
    20521 aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg
    20581 taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg cagttcagtg
    20641 ttgccctgac gtaccgcgat gccgaggcca gtaccgaaat aatctttatc ggtcacttta
    20701 tcgcccactg ccgccagttt tgggttatct ttcagccact cagtgaccac tgcggtgtca
    20761 ccaaatacgc tgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca
    20821 tacggaacgg tggtgatttc cgggtgctta tccataatga acttctggtg tgtcgtcccg
    20881 ttctgaacgc cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga
    20941 cccacaaaca gggcagagtt gtcatagtac ggggtagtaa acagtacctg cttttcacgc
    21001 tccggagtga tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg
    21061 atcaggctgt caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagt
    21121 gcctgtgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac
    21181 ggaggatagg aggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta
    21241 aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt
    21301 gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt
    21361 gttctacgat atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt
    21421 caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc
    21481 tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta
    21541 cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa
    21601 gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg
    21661 cacgggccag cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt
    21721 tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac
    21781 gacgcaaatc gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg
    21841 caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc
    21901 ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg
    21961 tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac
    22021 tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttga
    22081 cagaataatt atgcaatatt tatgctttaa aagttaaaag caaagcgcat tattcaataa
    22141 acatagcaca aaataacggg ggcggtggtc ggtgagcata aatgtcggca ttcctcacga
    22201 aatgccggat aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac
    22261 tgacggagcg tacccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg
    22321 acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgt
    22381 ttgagatcca cccgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg
    22441 gcaacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat
    22501 tcacgatgac tattatcgcg gctggcatcg ctaagttggg ttgccagttt gtcactcaaa
    22561 taggggcgca gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt
    22621 tgagccacac tgtcaggccc tccctggaca caggaaccgg tgcgggggcc gttatcttta
    22681 taagctggcg tgacggtggt acaggcactc agcagcagtg cacatggaat aagtagagat
    22741 agtgaacggt agcgcataat catttcctta ttagcttgct gtctgcgtca cactacgacc
    22801 aaacgctatc agattattta atacattgtt tttaatacaa ttctgaaaca atctgaacac
    22861 ctgattgggt ttgagtccat tgctggctac ccactcaagg ttgctcgtag ctgacttact
    22921 taaattaccc aaacaataga cagtgtaaac catttgtgag gttgagcgtg ttatatggct
    22981 gaaatgatca tttaaggtaa atatgatagt tctgaaatgg tggtataaat cctcctgtag
    23041 aggttaagag catctgcaag aagccagttt cacatttata acgcgtgata aatgaatgcg
    23101 gttattgtct aatcattaca aaatttttca catgcgataa gagatcctgc gattaagatg
    23161 attgccaaaa ttacgttgta tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac
    23221 tctcgataat attatttatg ctacaattgt gggtaaagaa tttaagctaa tgaatttgag
    23281 tgaagaataa attaaattgc ttcagtattt cgttaattaa tatgaataca aaaggcgttg
    23341 tgcttttata attataaagc gcgaggagtt cggtggatta tcgtttttct tggatgattt
    23401 ttaatatgat aaacaatata tctgatcaag catcatcatt tccaggcaca caactaaatc
    23461 aaagtgataa tttcctggat tcactgaggg aatttttcgc gatattaaat tccagtcgta
    23521 agggtgagtt gtctacatgg gataccatct acttgcatct gatattagca ataaatgctg
    23581 acagtgatct cataaaaaat gatgtgttgc tcgcggaaaa tattccatca gcgaattacc
    23641 aatttaatac attttttagc aatgctttcg agattgatgt taaaaaatat ctgggtaaat
    23701 cagaagataa cgaagttgaa ataaaagcgg gtaatgaaag aatctctatt ggaatacgta
    23761 atgtatcgaa tggaaaactt gaaagacaac agtttctttt cccactggac tatgagaata
    23821 aattacaaga acaattagat aagtatttta cgattgaatc gcacccatta ttatacaggt
    23881 ataccattgg tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact
    23941 ttaacaaaga acaatatata tcctttataa aggacgcatt tattcatttt tatgattata
    24001 gcaggcgata tgctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat
    24061 tgatgagcac tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta
    24121 cagaagaaga gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg
    24181 ctgatgataa ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg
    24241 cctttcaaga taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata
    24301 tttgtagcgt aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga
    24361 atgaagaagt tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa
    24421 gtaatttagt caatcttaaa acactactca atgatggttg ttttattaaa tatagtcatt
    24481 ttaatgacgt ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg
    24541 tgaatgaaag catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa
    24601 taacaaatag caatgtaata aacagtgcat tcaaaaatat ttcgtttaga tctgttaaaa
    24661 ttaataacgt aaatactcat tctttaaaat ttattaactg caattttttc aatgttgata
    24721 tgataagagt taatctcagc aaatgtcttt ttcatgaatg tagtatgcat ggtgtcaaga
    24781 taaaaccctg gttgcctgta aaatggacaa aagaattaat aagtgattat ttgtatggct
    24841 gtttactatc tctgtactct atcggtgcaa gggatatata taatatgaac gccgggaata
    24901 acgtgaaggt tgctgcggat gctttccttg aaattatttt ctctcttaaa aataaatatt
    24961 gtattaaact attaagcgct caggatcggg cgtttatata cgagtttgca agaatgatct
    25021 tcgcttatat taatgacaaa agcattgaga tcttattact ttcttgtttt gcggcggctg
    25081 accaaaaagc aatccagcgg tatagacctc agtcacaaga tggggaagac tttaggagtc
    25141 atttacaata taagttgcct ctttccgctc attaatgttg ttggtgcaac ataataagac
    25201 aggtaagcgg taccctataa tgaaaccaat atcgggttat gcaacggata atcgtaaaga
    25261 cttaagtggg tgtttttgac acaatgagat gatttcctaa actgatcaaa accttcttcc
    25321 caacatggca tatcagtccg ataatgcttt agagatcgaa ggcttaacaa acaaggagat
    25381 cgatcatgca attttctaca accccaactc tggaaggcca gaccatcgtt gaatattgcg
    25441 gtgtggtgac cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgccggta
    25501 tccgcgatat cgttggcgga cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg
    25561 agatcgcctt tgaggaatta ggctcccagg cgcgggcgct gggggccgat gccgtagttg
    25621 gtattgatat cgactacgaa acggtcgggc aaaacggcag tatgctgatg gttagcgtca
    25681 gcggtacggc ggtgaaaacg cgtcgatgag aagagtcttc tggctggtcg ctgccgctct
    25741 gttactggca gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga
    25801 tacccgacac caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac
    25861 cgcagatgat tttgatagct cgctggcgac actgactgat aagcaggtca gctcgcatta
    25921 tctgatccct gcggtgccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc
    25981 ggaacaagaa ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa
    26041 cgacacctct attggcattg agctggaaaa ccgtggctgg caaaaatcat ccggagtgaa
    26101 atattttgcc ccgtttgaac aggcacagat tcaggcgctt attccgctgg cgaaagatat
    26161 tattgcccgt tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca
    26221 gcgcaaagac gatccggggc cattatttcc ctggcagcaa ctggcgcagc aggggattgg
    26281 tgcctggccg gatgcgcagc gggtgaactt ttaccttgcc gggcgcgcgc cgcacacacc
    26341 ggtagatacc gcgtcattgc tggagctttt ggcgcgctac ggttatgacg taaaacctga
    26401 tatgacaccg cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac
    26461 gttatataac ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggagaa
    26521 atacgggcag gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc
    26581 gatgccttca tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat
    26641 atccagcgta aagtcaaaat tcaacttgga gacgccgtag tgggtcagcg gcggctcttt
    26701 tgctgacttg cggcctaaac gctccatgct gtgggcgatc atatccagca tcgggtaagg
    26761 gacggaacga atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgcg
    26821 cagtgtgcga tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca
    26881 ggcttcctgg cttgccagcc acattgcgtg cacggcattt tcatagtagg tcatatcgac
    26941 cagcgcactg ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag
    27001 acggggaata aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa
    27061 gcgcgtttgt ggattcgcct gcgaaagcat attgatcact tcttcgctgg ctgctttgct
    27121 gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct tctttaatat cgcgatggtg
    27181 gtgataatcg aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc
    27241 gacagcccat tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg
    27301 tgtcccccag ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat
    27361 cactttagct tgtgatgaaa ccagctcggt cagatccgtc ggaacaaact ctgcgcccat
    27421 tttttccagc aatttaccca ttgcttcgtt gcgaccgctc gcacgcacgc tgatgccttt
    27481 ctggcataaa aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac
    27541 cttcatatca atccactgtt gttgagaaaa tgacgtgcgc attcttccgt gatttccccc
    27601 atgatgcaat gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt
    27661 tctgccagtc tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc
    27721 atcaataacc agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg
    27781 atagtacgat agtcaccttt atcttccagg gtaaaacaca gtcgtcccag ccccggcgct
    27841 ttcatgccaa ataacaacgt aagttgcttt ttcggttcaa cgacaatcac tttccagcta
    27901 tccaccgcat cgccggtctg taaatattcg cgctccgggc ggcctttcgc cagattatga
    27961 ccgatcgcgc ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc
    28021 tctttaccgc cgatttggtt caccaccagc cataaagccg caaggctggc ggacgtttta
    28081 acggtaaacc ccgcctgttt ggcgaaataa ccgtactccg gtcgccagcg ggcaaaggcc
    28141 tgagcgtcgt agccccagtc gctggagttg accagttttt cttcctcttt caacgtgcta
    28201 cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca
    28261 tctgccagca gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg
    28321 gaagtaatca cattgagaaa ccacaccgaa atccagcggg tggggagggg gatggggatc
    28381 aaccagcggc gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc
    28441 acctctggtc cggcggcttc gaagatgcgg tgttcgttgg ctggatggtc taacagcgcc
    28501 accagatagt gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt
    28561 ggcggcgtta acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa
    28621 cctgcgccaa cgataattcc ggcccgaagt tcggtcactg gtacattcgc ttcacgaaga
    28681 atgtccgccg tagcctgacg agctcgcaga tgatccgact gctcatgtgg cggggcctgc
    28741 aacgaactga gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg
    28801 agagccacct ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga
    28861 aaatagacgg tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg
    28921 actttatggc aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc
    28981 gccgccagga tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag
    29041 ccactggcac cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa
    29101 tgcgcgccag tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac
    29161 caggcggaca atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa
    29221 cgcggcagca ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac
    29281 atccgcgcct gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg
    29341 caaccgtgcg acgttatttt tcagggcata catcccggcg gcagccagaa tcccggactg
    29401 acgcatcccg ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt
    29461 accgacgagt aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga
    29521 atcacaatat tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat
    29581 gtgcgcaccg tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt
    29641 caggtattca cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt
    29701 gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt
    29761 gccgtcggca gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc
    29821 ggcttcaaac agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca
    29881 gtgactgagc agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc
    29941 tttaccggaa agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta
    30001 aacgtcgtcc ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt
    30061 aacggtatca ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc
    30121 tttttacctt agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat
    30181 aattgcgcgc aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg
    30241 aatggctaat tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa
    30301 ggcgcgttgc agggcttcat caacttcaga ggctttttct acacggatac ccgtaatgcc
    30361 gcacgcttcg gcaatgcggg caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta
    30421 acctccggcc ttcatctcca tcgccacaaa gcccagcacg ctgttattaa agacgataat
    30481 tttcaccggc agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc
    30541 gccatcgccg cacatggcga ccacctgacg ttccggctcg gttgcctgcg cacccaacgc
    30601 ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc
    30661 gttcattttc agataacgtg ccgcccatac cgttggcgtg ccgacgtcac aggtaaagat
    30721 ggcgtcatcg gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt
    30781 ctcgctcggt ttagctaaat cgtccagccc tttgcgggca tcgcggtaat cttccagcgc
    30841 tttatccaga aacttgcgat cggttttttc ttccaccagt ggcagcaatg cgcgcagggt
    30901 tgatttgata tcgccgacca gcgccatatc taccttgcta tgcgcgccga tgctggctgg
    30961 attaatatca atctgaataa ttttggcatc ggtcgggtag aacgcacggt aggggaactg
    31021 agtaccgagt agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaatcc
    31081 gattaacccg gtcattccaa catcatacgg attatcgtat tcgacatgct ctttaccgcg
    31141 tagggcatga acaataggtg ctttaatttt cccggcaaac tcaactaact ctttatgcgc
    31201 ccccgcgcag ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc
    31261 cagtttgcgt aactcttctt cttccggcgt cacgactggc tgtggcgcat gataccagtg
    31321 cgtggttgcc ccttctggcg caggttttaa cgccacgtcg cccggtaaca caacaacgga
    31381 aacgccacgg ttaagcaccg ctttgcgcat agcaattgcc agcacttgtg ggatctgctc
    31441 cgggctggaa accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt
    31501 ttcctggaaa tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac
    31561 cggaacgtta ttgcggtggc aatcgaatag gccgttgatc aagtgcaggt tgcctgggcc
    31621 gcacgatccg gcacagaccg ccagttctcc tgaaagttgt gcttcagcgc cagcggcaaa
    31681 cgccgccact tcttcatggc gggtggacat ccactcgatg gtgcccatgc gattaagact
    31741 gtcgctaaga ccgttcagag agtcgcccgt gactccccag atgcgtttca cgcctgccga
    31801 ttcgagtgtt ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa
    31861 tgtgataacg gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg
    31921 aggcactaac ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc
    31981 gagaaccaaa tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc
    32041 ggcgtcggtc agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt
    32101 acagcagccg caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag
    32161 cgcctccagt agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt
    32221 gaatttcaga ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa
    32281 acgcgtcacg ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc
    32341 gcaggtcatc acggtacgtg aagctaagtt aggtacatct gccagcagtt cgcgagtgag
    32401 acgaccagcg ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt
    32461 acgccactca tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac
    32521 atcggcctgt ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc
    32581 gcagcctgcc gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc
    32641 catcgcgtcc gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga
    32701 gccgacaccg tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt
    32761 ggaggaaatg gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata
    32821 ttgcccggcg cgatatgggt agtgatcgtg gcaaatcagg gaaatcgtcc acacatccgg
    32881 cgtttcttgc gtaatgtgat gaacctgcat ccgccacggg cattgattcg ttggcatcgt
    32941 catcgacaaa ctccttacgc gctcaacagt tgcttcatgt cttcttcaac agtggtgata
    33001 gaacgcaggc cgaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaactaggt
    33061 gcagtcgggc cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgaca
    33121 atcgctttct gttcaaacca ggagagcacc agcgacagcg gcagatcgtt cacaccacag
    33181 cccagtttct ctgccagagt gacagccaga ataattgctg agtaagcatc gttacattga
    33241 cctgcatcta ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga
    33301 tatttaccac aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg
    33361 gtgaagtagt ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc
    33421 agtttttcac ggctaaccag atcaatcagc gtatcggctg caccaagcag cgtctggcga
    33481 ccaaagccca cggtgatcag gtgaggaatt tcgctgtacg ggaagcccgc catttgttgc
    33541 gcctgggcga tgaccgcaga gaaatcttcg ccatccagat gacgcacgcc aggccagcca
    33601 acgatgctgc gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag
    33661 ttcgaggtca tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag
    33721 ccgctgccgt agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca
    33781 ggcagcattt cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg
    33841 ttgtagagat ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg
    33901 acgttgacct gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg
    33961 ctcatcactt tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata
    34021 tcggcaggcc aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg
    34081 tcgtattgac cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac
    34141 aggcacagca gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg
    34201 gtaaattctg ctgcctgacg ttgcagctcg ccgagatcat cgctcaccag ttgcaggtca
    34261 gccatcgggt tatcgacgcg cgcgttggca tctacagcca ggcattgtgc tttcagcgcc
    34321 tcgcgcaagg caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg
    34381 ttggtcaggg ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg
    34441 ccgtattcac gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa
    34501 tcctgaaggt cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg
    34561 tttcctgccg gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt
    34621 aaagttatat ttaatataca tgtttaaggt taagatgctt aacgcgggga taaaagggat
    34681 ttttcatgca actttaaggg agattgattt agcgcaattt tggcggcagg gatctaccgc
    34741 cagagaggta ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa
    34801 aaccgtgaac aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca
    34861 gggtgaaatc cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa
    34921 tcagcccagg gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg
    34981 cgctcccgat taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc
    35041 cgaaaccgga ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa
    35101 ttaaaccacc aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat
    35161 taaggacaat ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa
    35221 gtagcaaaat taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac
    35281 tcagaccaat ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca
    35341 tcgcaatacg cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct
    35401 ccagccacat cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat
    35461 aatgcagaat cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca
    35521 taaaaaagag aataaggtaa accatccagc ttaatagctg attaataact tttaacgcag
    35581 cttgttggcg aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc
    35641 cagaaaacat gaaaatccag tccttgcaaa gaagaagtcg aaatgcgcga tgacacacta
    35701 ctgaaagcgg aaggacgagt aaagttgcaa ttaaaaggaa atgttatgca tcagaggggg
    35761 atttttaatc ctatctgagt gtttagcatt gttgcgctcc tttcatgtgc aatgccatat
    35821 aaagccactg gcggagggag cgcgcagggg gcagccagtc gccgccgccc cctgcacccc
    35881 cgggctctgg cgaacaaaat cgccgctgcg cggtaccttc agcttatccc cttcggcaac
    35941 caggtcgggc gcgaggtaac gtcctgtaag acgcgccctc agcccacatc catgtgggct
    36001 gccccggcct tcggggaacg cttcagcgat tttgacgcca ccaatacccg tgcggtttgt
    36061 aatgtcgaag ataaaacaac cagggcagat aaaatgccaa tgctaattaa tcacgctttt
    36121 ccagcagagt ccggtaaatc agaccaccga taatgccgcc gacaattggc accacccaga
    36181 agaaccacag ttgttctaat gcccagccgc cctggaagat agcaaccgcg gtgctgcgcg
    36241 ccgggttaac agaagtgtta gtcaccggaa tactgattaa gtgaatcagg gttaaggcca
    36301 gaccaatagc gatcggcgca aaacctgccg gagcgaattt gtcggttgca ccgtggatca
    36361 ctaacaggaa gcccgcactc aataccagtt caactaccag cgcggaaagc atggaataac
    36421 cgcctggtga atgctcggca taaccgttag aagcaaaacc gctggctgcc gcgtcaaaac
    36481 ccgttttacc actggcaatt aaatacagca gcgccgctgc aacaataccg ccgacaacct
    36541 gggcaatcac gtagccaacg acttcttttg ccggaaaacg tccgccagcc cataaaccaa
    36601 tagtgaccgc cgggttaaaa tgaccaccag aaatatgacc aacagcgaag gccatcgtca
    36661 gaacggtcaa accaaatgcc agcgccacgc cggcaaaacc aatgcctaat tccgggaagc
    36721 ctgcggccag tacagcacta ccacagccac caaaaacaag ccagaaagta ccaaaacatt
    36781 cagctgctaa ttttctgaac atatccacct caattaaaaa ttgaccctgt gaaaaatatg
    36841 gtcgttttat agggtcgtcg taaaaaatga cgacggaaat aatgcgcggc tattttaaaa
    36901 acgaaggcga gtcattcacc agataaataa atccagtaaa tttgatttag ggcaacagcg
    36961 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcaagcaaaa gatttaatcc
    37021 tttaggcgta ataaaaaata atttatcatg ctaattattt gattttgttg tttttgcggg
    37081 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca attgccatgc
    37141 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
    37201 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
    37261 gcggtatcaa ccgtttgtcg ttgatgctcg aacaaaacaa cgtcctgatt ggggagaacg
    37321 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
    37381 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
    37441 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
    37501 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
    37561 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
    37621 ataaagacgg acatccgatt gatgttgagg atattaacga tcaggcacgc catctggtgc
    37681 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
    37741 cggtgccaaa tgtccccaat gtggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
    37801 agttgtcctc acatccgcaa aatctctctg atggacagat tcgtcaggga ctttccgcaa
    37861 tggtgcagtt gcttgagcat tatttctctg agcagggggc tggacaggcg cgatatcgtt
    37921 taatgcggcg gcgggccagc aatgagcaac gaagctggcg ctatctggac atcatcaacc
    37981 ggatgattga ccgacctgga gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
    38041 ctttattgca ggcaaaaggc acattgcgac tggataaaga cgcgcgtccg ttattgctaa
    38101 ttgaagatcc agaaacccgc ctgcatccga ttatgctttc cgttgcctgg catctgttga
    38161 atcttttgcc tttacagcgt attgctacca ccaactctgg cgagctgctt tcgttaactc
    38221 cagtggaaca tgtttgccgc ctggtgcgtg aatcttctcg tgttgccgcc tggcgtctgg
    38281 ggccaagtgg cttgagcacc gaagacagcc gccgcatctc ttttcacatt cgttttaatc
    38341 gtccgtcatc gctgtttgct cgctgctggt tgctggtgga aggggaaacg gaaacctggg
    38401 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
    38461 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
    38521 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
    38581 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
    38641 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
    38701 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
    38761 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
    38821 taccaacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
    38881 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
    38941 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
    39001 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgactcc cagaacgcgt
    39061 tgtaatcagc atggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
    39121 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
    39181 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
    39241 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
    39301 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgga
    39361 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
    39421 actgctcacc gtttttgcct tccagtttag ccagttgcag cccgggggta ttcaaatata
    39481 agctgaattc ttccgccgac atacacccgc ggagtaacgc ataatggtaa cgtaacgcct
    39541 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
    39601 gtcgcggctg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
    39661 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
    39721 agcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
    39781 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
    39841 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
    39901 tgtgattact aacgtaaatt ttaggatttt gttgatattt cgttgaagtt aatgacccgg
    39961 attggcatgt ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
    40021 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
    40081 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
    40141 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgta ggcgcgcagg tcagcggtca
    40201 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
    40261 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
    40321 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
    40381 tcagcaacgt ctggcacaaa cgcaagcggt ttcactgcag gatctcgata ccgccgcgac
    40441 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
    40501 ggcctctctc gatacggcta aaaccaatct cgattacacc cgtatcgttg ccccgatggg
    40561 cggggaagtg acgcaaatca ccactctgca aggccagacg gtgattgccg cacagcaagc
    40621 accgaacatt ctgacgctgg cagatatgag cactatgctg gtaaaagcgc aggtttctga
    40681 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtgc ttggcgatcc
    40741 actgacgcgc tacgaggggc aaatcaagga tgtgctaccg acgccggaaa aggtaaacga
    40801 cgctattttc tattacgccc gttttgaagt acccaacccc aatggtttgc tgcggctgga
    40861 tatgaccgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgttga cgatccctct
    40921 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
    40981 tgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
    41041 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
    41101 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg tgatgagcag
    41161 gttgaggtgc tgaagggcat cagcctcaat atttatgcgg gtgagatggt cgcgattgtt
    41221 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
    41281 accagcggca cctatcgcgt agccggtcag gatattgcca cgctggacgc cgatgcgctg
    41341 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
    41401 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ccggtcttga gcggaaacag
    41461 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg cacagagtat
    41521 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
    41581 aacggcggtc aggtaattct tgccgatgaa ccgaccggcg cactggacag ccattctggc
    41641 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
    41701 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
    41761 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
    41821 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
    41881 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
    41941 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
    42001 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
    42061 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
    42121 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
    42181 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
    42241 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
    42301 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
    42361 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
    42421 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
    42481 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
    42541 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
    42601 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
    42661 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggcgg tattggtgta
    42721 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
    42781 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
    42841 ctggtcggtg gcgcgttggg aataacactg tcactgttaa ttgctttcac cttgcagctt
    42901 ttcttacccg gctgggagat tggtttttca ccgttggcgc tgctgctggc gtttctctgc
    42961 tcgacggcca ccgggatttt atttggctgg ttacccgcac gaaatgcggc acgactggat
    43021 ccagtagatg ctctggcacg ggagtaattt ttgagataaa aatgccagcc gatcgggctg
    43081 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
    43141 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
    43201 aacggattgt ccagctttta gcgttctgta accatccatc tgaatggtgg aataatgagc
    43261 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaacctttgg cattgttgaa
    43321 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
    43381 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
    43441 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
    43501 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
    43561 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
    43621 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
    43681 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
    43741 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
    43801 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
    43861 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
    43921 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
    43981 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
    44041 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
    44101 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
    44161 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
    44221 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
    44281 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
    44341 tctggtcgct atcttcagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
    44401 agtcagccgt ctcgatgtgg tgaactttat ctctcatggc acgcgtaaag acgagccgac
    44461 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
    44521 tatggagaat ttcacgacga acctgaacca gcttgcgcgt gtgggcggaa tcgacccact
    44581 aattggtcgt gagaaagagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
    44641 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcag aaggtcttgc
    44701 ctggcgaatt gttcagggtg atgtgccgga agtgatggct gactgtacga tttactctct
    44761 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
    44821 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
    44881 caccattatc ggtgcgggtg cagcgtctgg tggtcaggtc gatgcggcta acctaatcaa
    44941 accgttgctc tccagcggta aaattcgcgt aattggttcg acaacctatc aggagttcag
    45001 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
    45061 accgtcgatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
    45121 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
    45181 cattaacgat cgtcatctgc cggataaagc cattgatgtt atcgacgaag cgggcgctcg
    45241 cgcacgtctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
    45301 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
    45361 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggccaggata aagccattga
    45421 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
    45481 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
    45541 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
    45601 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
    45661 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
    45721 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
    45781 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ctaacgccgg
    45841 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
    45901 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
    45961 ctggttcgat catctgtcaa ctgacgtgat ccatcaggtg gtggataaat tcatcgtcga
    46021 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
    46081 ctggctggcc gagaaaggtt acgaccgggc aatgggcgca cgtccgatgg cgcgtgtcat
    46141 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
    46201 cggtcaggtg acggttgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
    46261 tgcacaaaag cacaaggcgg aagcagcgca ttaatctgat tgtcaggtag gttggtgaag
    46321 tccgtaatct cgtcaggggt tacggacttt ttatttatgg ggtggaacgg tgaaaaccct
    46381 atttttggag gtgaaggtaa gttgttgata attagtgctg ctggaaggta aggataaaaa
    46441 agggtgctgc aggagaatgg gatggttttg ctttattaac aacgggctaa acgtgtagta
    46501 tttgagttca ctgccgtaca ggcagcttag aaatcgacgg ggtgcggtaa aacctttgcg
    46561 aacgcgttca ctgccgtaca ggcagcttag aaattcacag gtaacatact ccacccgccc
    46621 accatgttca ctgccgtaca gacagataaa atgcgaaaaa aaagctcgca ctttcgtacg
    46681 agctcttctt taaatatggc ggtgaggggg ggattcgaac ccccgatacg ttgccgtata
    46741 cacactttcc aggcgtgctc cttcagccac tcggacacct caccaaattg ttttgctacc
    46801 aaacctcatg ggtggcaacg gggcgctact atagggagtt ggagtaaaac ggtcaagaag
    46861 aattttaatg ataattattg tttgctcata ctgtaaacaa cttgtgcagt atatctacat
    46921 cgagacaagt tatggactta tacttccaaa gtacttcata catatcacaa aataaaaagg
    46981 ccggttaaac cgacctttta ctcgttcttt ctcttcgccc atcaggcggt aaaacaatca
    47041 gcgactacgg aagacaatgc ggcctttgct caggtcgtac ggggtcagtt caacagtcac
    47101 tttgtcgccc gtcaggatgc ggatgtagtt tttgcgcatt ttaccggaga tgtgtgcagt
    47161 aaccacgtga ccgttttcta actctacgcg gaacatggta ttaggcaacg tttcaagaac
    47221 ggtaccttgc atttcaatat tgtcttcttt ggccatctaa tcctctgggg tatcactacc
    47281 gtaatttgaa ccggcaagat aatgccgaag ttctgtaaat aagtaaagat ttgcgcgcta
    47341 aatcgcaaca aacaggttcg gcacattact ccgaaaacac acggctaagc cgcaccaaaa
    47401 gcgcaacgta taagggagcg gtgagataaa cgatgggcgt tacctgacgc gaaaaattcc
    47461 ttatcggcag cggggtaatg agcgtaacca actctgcgac cgcaattata acactctggg
    47521 gagaaatgtg ccgaaaacat tcattcttgt ggtgaaaaca agcaccgtgg tacccagaaa
    47581 ttattcggca atcgtccgag gcgcatttga ttgagataat taaggtaatc ccggcgggga
    47641 atttcgcagg caccaagcga tgctgtgtga tcgttaagga cctggcagtc gataagctta
    47701 ccgccatgac cgataaattc ctcacagaat accagaagcg ccgttttaga cgcattttcc
    47761 atccggctga acatggactc gccacaaaat agcgttccct gggccacgcc gtacataccg
    47821 ccgacaagct catcttcacg ccagacttca atggagtggg catgaccgag ttcgtgaagg
    47881 cgatggtagg cttcgaccac gccacgcgtg atccaggttc cttcttcgcg atcgctggca
    47941 cagccttcaa tgacctgacc aaaagcgtaa ttcatcgtga cacgataggg cgagcgttta
    48001 tgaaatcgct tcatactacg gctgatatgc agtgattctg gccatagcac cgcgcgggga
    48061 tcgggcgacc accagaggat ggggtcgcct ggagaaaacc acggaaaaat accacgctgg
    48121 taagccatta acaggcgcgc agggctaaga tcgcccccaa gtgccagcag gccgttaggc
    48181 tcacgtaatg cgccttccgg ggaagggaag gctattgaat ggcgagaaag ctgaaccagg
    48241 cgcatgaccg caaaactcca cgcaagtcgg atcgttcaat aatagcttac aaaccctgct
    48301 tgaactggta ataacgcccc tgtctggcaa gcagttctgc gtgagtacct tgctcaataa
    48361 tttgcccgtt gtccatcact attatttgtt ggaaacgaga gagtccgcga agtcgatggg
    48421 tgaccattaa caccgttttc tcacgcatca tttttgcaag caattcaagg atctggcttt
    48481 cggttgtggc atctaagcct tcggtaggtt catccagcaa caccagtggc gcatcatgta
    48541 acagcgcacg ggcgatagcc agacggcgca gttcaccacc ggagagctgg cgtccgcctt
    48601 cacctaacca actgttgaga cctgcatcct cgagcagctt ctccagacca acgcgacgca
    48661 agatctccga cagagcctca tcactactgc caggcgaggc gagtaacaga ttatcacgca
    48721 gcgtggcgct aaacagatgc actcgctgag gaacaacgct gatggtctgt cgtagagccg
    48781 cttcattcag gctggctatg gggctatcgt taagcaaaat ctcgccctgt tgcgggtccc
    48841 atgcgcgggt cagcagttgt aacagtgttg atttgccgca tccggttcgc ccgagaatcg
    48901 ctatatgttc cccggcgttt acctgaagag aaatcccttt aagtgcctgt tgggattgct
    48961 ccggataagt gaactgaaca tcccgtaatg tcagcgaaac gcgatcggca acacgagttt
    49021 gggtatcagg gaaggtgacc tccggttttt gatccgttaa gtcagagata cgtacggcag
    49081 aggcaatgac ttgccccaga tgctgaaatg cacccgttac tggtgccagt gcttcaaacg
    49141 cggctaacgc acaaaagaca aacagggcaa ttaatgcgcc gggttgagca ttgccgccaa
    49201 cgccgccaga cgccatccac agcatcagga tcaccgctaa cgcgccaatg agcagcatta
    49261 tcgcttgcga caatgcggtc agttcagatt gacggcgttg cgcttccagc cattgaattt
    49321 ctgtattctc tagttgcgtg cgataacgat cgctggcacc aaaaatggtc agctcagctt
    49381 gcccttgcag ccaggccgtc agttgttggc gatactgtcc gcgaagatga gtcagatttt
    49441 gcccggtgct ttttcccgca cgataaaaca gcggtggcat caggaaaagc gtcagtaaca
    49501 taacgctgcc cagcgtaaag gcgagggtga aatcaaggaa acttaacccg attgtcacca
    49561 ccataatcac cacaaaagcg cccaccagcg gcgagataac gcgcaggtaa agatgatcga
    49621 gcgtatcaac atccgccacc acgcggttga gcaattcgcc ctgacgatag cgcgccagtc
    49681 cggcagggga gaggggcaac aatttgctga aggtgtaaat gcgcagatgc tgcaacacgc
    49741 ggaaagtcgc gtcgtgactt accagacgtt caaaataacg cccggcagta cgggtgattg
    49801 cggcaccacg cacgcccgca gcgggtagca tatagttgaa gctgtacagt ccggcaaccc
    49861 ccgcaaccgc tgaggccgag aggaaccagc cggaaagtgt caacagaccg atactggcga
    49921 gcagcgtcac aattgccagc acaataccaa gacttaacat ccatttatga cgtttataca
    49981 gtgccagata gggtagcaaa gcgcgcattt aaatctcctc ctgacgatgg gccagtaatg
    50041 tggcgaatgg gccaccagcc acacttaatt ccgcgtaacg tccttgctca ataatccggc
    50101 catcctgcat aacccaaatg acatcccagt cagcaagatc ttctaactgg tgggtgacca
    50161 ttaacgttgt ctggcgcaga gaggcggcat tcagcgcctc cattacgcgc tgttcactgt
    50221 gagcatcaag gctggcagcg ggttcatcca acagtaatag cgaacaggga tttagtaacg
    50281 cacgggccac cgccacgcgc tgcgcctgcc ccacggaaag gcgggcagcc tggtcgccaa
    50341 caggtgtatc aacgccttgt ggcaggagcg gtagaaactc gctgacccag gcgttatcca
    50401 gcgctgcttg taattcttgt tcgctggcat caggtcgcgc cagtagtacg ttatcccgca
    50461 attttgctgc cggtaattgt gggttttgcc caacccagga gagatgttta cgccatgatt
    50521 ctggtgataa atcacgtaat tctatcccgt tgattcgtaa tgatccctga tatgagagaa
    50581 aaccagaaag cgcgttcagc agtgagcttt tacctgaacc gctgcggcca accaacactg
    50641 cacgttggcc tgctggcaaa gtaaagttca gcggtccggc cagcgtttta ccttccggcg
    50701 acgtgataaa cagatcctcg gcctcaatgg tcagcggatc ggtcaatgct aattccgcct
    50761 caccgcgctg cggatgggcg agcggggttt ccataaacgt tttcagactg tcagctgcac
    50821 caacagcctg ggctttagca tgataaaacg taccgagatc gcgtaatggc tggaaaaact
    50881 ctggcgcaag gatcagggcc agaaaacctg cagccagcgt caccccagta tcgtagtgac
    50941 caaaatccag ctcgccgaga taggaaaaac caaagtagac cgccaccaga gcaattgaca
    51001 gcgaggtaaa aaattcgaga atgccggagg ataaaaacgc cagtcgtagc acttccattg
    51061 tccgttggcg gaaatcttcc gaagcagaac gaatactttc aatttcagct tcaccacgac
    51121 caaaaatacg caatgtttcc atgccgcgca ggcgatcgag gaaatgccca cttaagcgag
    51181 caagagcgag aaagttacgc cggttagcat cggcagcccc cattccaacc agcgccataa
    51241 acaacggaat tagcggtgct gtgcccagca gaatgagcgc cgcagcccag ttagagggga
    51301 agatcgccac cacaatcagc aacggcaccg acactgccag cgccatttgc ggcagatagc
    51361 gtgcatagta atcatgcata tcgtcaattt gctcgagtac cagcgtcgcc cagctccccg
    51421 caggtttacc ctgaatccac gctggccctg cttgttgcag acggtcgaga acctgacggc
    51481 ggatggcaaa gcggatatgc tgcccggcgt gataacccac ccgttcgcgt aaccagacca
    51541 cccatgcgcg caatacaaag gtcagaacca gtaacgtaaa gggaagcagc agggcttcac
    51601 ggggaatatt ctccataatc atatgttgca gaattcgcgc catgaaccag gcctgggcaa
    51661 tgatcaatat gccgcttaca aagcccagca gacgagaaat attcagccaa cgttgggaga
    51721 tgacgctttg ctgttttaac cagcgggtta actctttttg acgagattta ttcattgcac
    51781 gcttagcagg tgagttatca gaattatttg cagagcaatg ttacaacggg gaaaaaataa
    51841 aggcgaccca tagtcgcaag gtgtcgcctt ctttactttt gttactgatt tgtaaaatta
    51901 ttttgcgtca gctaaaccat cgaggtagcg ttccgcatca agtgctgcca tgcagccagt
    51961 accggccgaa gtaatggcct ggcgataaat gtgatccatc acgtcgcctg cggcaaagac
    52021 gccaggaatg ctggtctggg tggcattacc atgaataccc gactgtactt tgatgtagcc
    52081 gttttccagt tccagctgcc cttcgaaaat cgcagtattc gggctgtgac cgatagcaac
    52141 aaacagaccg gcaacgtcga gtgactcgat gttatcgctg ttttgcgtat cgcgcagacg
    52201 aacgccagtg acacccattt gatcgccggt cacttcttcc agcgtacggt tggtgtgcag
    52261 aatgatgttg ccgttctcca ctttatccat cagacgctta ataaggattt tttccgcgcg
    52321 gaaaccgtca cggcggtgaa tcagatgcac ttccgaagcg atgttagaca gatacagcgc
    52381 ctcttcaacc gcggtattgc cgccgccgat gaccgcaact ttctggttgc gatagaagaa
    52441 accgtcgcag gttgcacaag cagaaacccc acggccttta aaggcttctt cagagggcag
    52501 gccgagatag cgtgcagaag ctccggtggc aataatcagc gcgtcgcaag tgtattcgcc
    52561 gttatcgcca ttcagacgga acggacggtt ttgcagatcc accttgttga tatgatcaaa
    52621 aatgatctca gtttcaaact tggtggcatg ttcgtgcatg cgcgccatta ataacggacc
    52681 ggtcagatcg tttggatcac caggccagtt ttccacttcc gtggtggtgg tcagttggcc
    52741 gcctttttcc atgccggtaa tcagcacagg ttgcaggttg gcgcgcgccg cgtagacagc
    52801 agcggtgtat cccgccgggc ctgaacccag gataagcagt ttactgtgtt tggtcgtgcc
    52861 catgagatcc ccatagttgt tggcagacaa tgggcaggat tgtagggaat ttacagacgt
    52921 aaaaaaagag tatgacgatt ttgttaacaa tttgtgcaat cggcagcatc gataagcagg
    52981 tcaaattctc ccgtcattat cacctctgct acttaaattt cccgctttat aagccgatta
    53041 aatgatgaat aaacgcccct gttaatgaat atctggcatg ttgtactaaa aatcgatgtt
    53101 ttgctttgac aatcccctgg tgttttgcga aaacattcga ggaagaaaaa aaacagtatt
    53161 cttatatgcg cataaccatg tatgtaaata ccatgtttac cgtgctagtg aaatctacgt
    53221 atggcgtgga cagacgccat tcgtgatgtc gatagctgcc acaaggcaac ggtcttctca
    53281 ccgtagaccc aggcattgcg cgccgtgaat cttcatgatt tcggtctatc gtgacgggta
    53341 gcgactctga acagtgatgt ttcagggtca gacaggagta gggaaggaat acagagagac
    53401 aataataatg gtagatagca agaagcgccc tggcaaagat ctcgaccgta tcgatcgtaa
    53461 cattcttaat gagttgcaaa aggatgggcg tatttctaac gtcgagcttt ctaaacgtgt
    53521 gggactttcc ccaacgccgt gccttgagcg tgtgcgtcgg ctggaaagac aagggtttat
    53581 tcagggctat acggcgctgc ttaaccccca ttatctggat gcatcacttc tggtattcgt
    53641 tgagattact ctgaatcgtg gcgcaccgga tgtgtttgaa caattcaata ccgctgtaca
    53701 aaaacttgaa gaaattcagg agtgtcattt agtatctggt gatttcgact acctgttgaa
    53761 aacacgcgtg ccggatatgt cagcttaccg taagttgctg ggggaaaccc tgctgcgtct
    53821 gcctggcgtc aatgacacac ggacatacgt tgttatggaa gaagtcaagc agagtaatcg
    53881 tctggttatt aagacgcgct aacacggaac aggtgcaaaa tcggcgtatt ttgattacac
    53941 tcctgttaat ccatacagca acagtactgg ggtaacctgg tactgttgtc cgttttagca
    54001 tcgggcagga aaagcctgta acctggagag cctttcttga gccaggaata cactgaagac
    54061 aaagaagtca cattgacaaa gttaagtagc ggccgccgcc ttctggaggc gttgctgatc
    54121 cttattgtcc tgtttgccgt ctggttgatg gctgccttac taagctttaa cccttcggac
    54181 cccagctggt cgcaaacggc ctggcatgaa cctatccata atttaggtgg gatgcccggt
    54241 gcgtggttgg cagatacgct gttctttatt tttggcgtga tggcttacac cattcccgtc
    54301 attattgtcg gcggttgttg gtttgcctgg cgtcatcagt ctagcgacga atacattgat
    54361 tattttgccg tttcgctacg catcattggc gttttggcgc tcatccttac ctcctgtggt
    54421 ctggcggcaa tcaacgctga cgatatctgg tattttgcct ccggtggcgt cattggcagc
    54481 ttactaagca ctacgctaca accactgcta cacagtagcg ggggaactat tgcgctgctc
    54541 tgcgtttggg cagcgggcct gacgttgttc accggttggt catgggtgac cattgctgaa
    54601 aaactcggcg gctggatttt aaacattctc accttcgcca gtaatcgtac ccgtcgcgat
    54661 gatacctggg tcgatgaaga tgagtatgaa gacgacgaag agtatgaaga tgaaaatcac
    54721 ggcaaacagc atgaatcacg ccgtgcccgt attcttcgcg gcgcgctagc gcgtcgtaaa
    54781 cggttggcgg aaaaattcat taatccgatg gggcggcaaa cagacgctgc gttgttctcc
    54841 ggtaagcgga tggatgatga cgaagagatt acctacactg cacgcggtgt ggctgtcgac
    54901 ccggacgacg tcctattttt gggcaatcgt gcaacgcagc cagaatatga cgaatacgat
    54961 ccattattaa acggtgcgcc aattaccgaa cctgtcgctg tagcagctgc tgctaccacg
    55021 gcgacacaaa gctgggctgc gccggttgaa cctgtgactc agacgccgcc tgttgcctct
    55081 gttgatgttc caccttcgca acctacagta gcctggcagc ctgtaccggg tccacaaacg
    55141 ggagagccgg ttattgctcc tgcaccggaa ggttacccac agcagtcaca atatgcgcag
    55201 cctgcagtgc aatataatga gccgctgcaa caaccagtac agccgcagca gccgtattat
    55261 gcacctgcag ctgaacaacc tgcgcaacaa ccttactacg ctcctgcggc ggaacaacct
    55321 gttcagcaac cgtattacgc aactgcgccg gaacagcctg cgcaacagcc gtattatgcc
    55381 cctgcgccag aacaaccggt ggcaggtaac gcctggcaag ccgaagagca gcaatccatt
    55441 tttgctccac agtctacata ccagactgag caaacttatc agcagccagc cgctcaggag
    55501 ccgttgtacc aacagccgca acccgttgaa cagcagcctg ttgtggagcc tgaacccgtt
    55561 gtagaagaga caaaacccgc gcgtccgccg ctttactatt ttgaagaagt ggaagagaag
    55621 cgagcccgtg aacgtgaaca acttgcggcc tggtatcaac cgattccaga accggttaaa
    55681 gaaccagaac cgatcaaatc ttcgctgaaa gcaccttctg ttgcagcagt acctccagta
    55741 gaagccgctg ccgctgtttc tccgctggca tctggcgtga aaaaagcgac actggcgacg
    55801 ggggctgccg caaccgttgc cgcgccagtc ttcagtctgg caaatagcgg tggaccgcgt
    55861 ccgcaggtca aagaggggat tggcccgcag ttgccacgac cgaaacgtat ccgcgtgcca
    55921 actcgtcgtg aattggcgtc ttacggtatt aagctgccct cacagcgtgc ggcggaagaa
    55981 aaagcccgtg aagcccagcg caatcagtat gattctggcg atcagtataa cgatgatgaa
    56041 atcgatgcga tgcagcagga tgaactggca cgtcagttcg cccagacaca gcagcaacgc
    56101 tatggcgaac agtatcaaca tgatgtgccc gtaaacgcag aagatgcaga tgctgcggca
    56161 gaggctgaac tggctcgtca gtttacgcaa actcaacaac aacgttattc cggcgaacaa
    56221 ccggctgggg cgaatccgtt ctcgctggat gattttgaat tttcgccaat gaaagcgttg
    56281 ctggatgatg gtccacacga accgctgttt acgccaattg ttgaacctgt acagcagccg
    56341 caacaaccgg ttgcaccaca gcagcagtat cagcagccac aacagccggt tgcgccacag
    56401 ccgcagtatc agcagccaca acagcaggtt gcgccgcagc cacaatatca gcagccgcaa
    56461 caaccggttg cgccacagca gcaatatcag cagccgcaac aaccagttgc gccgcagccg
    56521 cagtatcaac agccgcaaca accggttgca ccgcagccgc agtatcagca gccacaacag
    56581 ccagttgcgc cacagccgca ggataccctg cttcatccgc tgttgatgcg taatggcgac
    56641 agccgtccgt tgcacaaacc gacgacgccg ctgccttctc tggatttgct gacaccgccg
    56701 ccgagcgaag tggagccggt agataccttt gcgcttgaac aaatggcgcg tctggtggaa
    56761 gcgcgtctgg ctgatttccg tattaaagcc gatgtcgtta attactctcc ggggccagtt
    56821 atcactcgct ttgaacttaa cctggctcct ggcgtaaaag cggcgcgcat ttctaacttg
    56881 tcacgggacc ttgcccgttc actttcgacg gtggcggtgc gtgtcgttga agttattcct
    56941 ggcaaaccct atgtaggtct ggagttacca aataaaaaac gacaaaccgt ttatctgcgc
    57001 gaagttttgg ataacgccaa attccgcgat aatccgtcgc cattaaccgt ggtgctgggt
    57061 aaagatatcg ccggtgagcc ggtggttgcc gatctggcga aaatgccgca cttgttggtt
    57121 gcggggacta ccggttccgg taaatctgtc ggtgtgaacg cgatgatcct gagcatgctt
    57181 tataaagcac agccagaaga tgtgcgtttc atcatgatcg acccgaaaat gctggagctt
    57241 tcggtttatg aaggcattcc gcacctgtta acggaagtcg ttactgatat gaaagatgcc
    57301 gccaacgcgc tgcgctggtg tgttaacgag atggagcgtc ggtataaact gatgtctgcg
    57361 ctgggtgtgc gtaatctggc gggttataac gaaaaaattg ctgaagccga tcgcatgatg
    57421 cgtccgattc cagacccgta ctggaagccg ggtgacagta tggatgccca gcatccggtg
    57481 ctgaaaaaag aaccatacat tgtcgtgttg gttgacgaat ttgccgacct gatgatgacg
    57541 gtaggtaaaa aagtggaaga gctgatagca cgtctggcgc aaaaagcccg tgccgcgggt
    57601 atccacctcg tactggcaac tcagcgtcca tcggttgatg ttattactgg tctgattaaa
    57661 gcgaatattc cgacccgtat cgcctttacc gtatccagta agattgactc acgtaccatt
    57721 cttgatcagg ctggcgcgga atcactgctg ggtatggggg atatgctcta ctctgggccg
    57781 aactccacgt tgccggtacg tgtccatggt gcttttgttc gcgatcagga agttcatgcc
    57841 gtggtgcagg actggaaagc gcgtggtcgc ccacagtatg ttgatggcat cacctccgac
    57901 agcgaaagcg aaggtggtgc gggtggtttc gatggcgctg aagaactgga tccgttgttc
    57961 gatcaggcgg tgcagtttgt cactgaaaaa cgcaaagcgt caatttctgg cgtacagcgt
    58021 cagttccgca ttggttataa ccgtgcagcg cgtattatcg aacagatgga agcgcagggg
    58081 attgtcagcg aacaggggca caacggtaat cgtgaagtgc tggccccacc gccgtttgac
    58141 taattaatgc attgccggat aaggcgcggt agcgtcgcat ccggcactct atcaactgaa
    58201 aattcagtat tttcttcttt cctcaagctg attattagcc tggaatagag agtagaggga
    58261 actcccgatc gggagtgacg taatttgagg aataatgatg aaaaaaattg ctatcacctg
    58321 tgcattactc tcaagcttag tagcaagcag cgtttgggct gatgccgcaa gcgatctgaa
    58381 aagccgcctg gataaagtca gcagcttcca cgccagcttc acacaaaaag tgactgacgg
    58441 tagcggcgcg gcggtgcagg aaggtcaggg cgatctgtgg gtgaaacgtc caaacttatt
    58501 caactggcat atgacacaac ctgatgaaag cattctggtt tctgacggta aaacactgtg
    58561 gttctataac ccgttcgttg agcaagctac ggcaacctgg ctgaaagatg ccaccggtaa
    58621 tacgccgttt atgctgattg cccgcaacca gtccagcgac tggcagcagt acaatatcaa
    58681 acagaatggc gatgactttg tcctgacgcc gaaagccagc aatggcaatc tgaagcagtt
    58741 caccattaac gtgggacgtg atggcacaat ccatcagttt agcgcggtgg agcaggacga
    58801 gcagcgcagc agttatcagc tgaaatccca gcaaaatggg gctgtggatg cagcgaaatt
    58861 taccttcacc ccgccgcaag gcgtcacggt agatgatcaa cgtaagtaga ggcacctgag
    58921 tgagcaatct gtcgctcgat ttttcggata atacttttca acctctggcc gcgcgtatgc
    58981 ggccagaaaa tttagcacag tatatcggtc agcaacattt gctggctgcg gggaagccgt
    59041 tgccgcgcgc tatcgaagcc gggcatttgc attctatgat cctctggggg ccaccgggta
    59101 ccggcaaaac aaccctcgct gaagtgattg cccgctatgc gaacgctgat gtggaacgta
    59161 tttctgccgt cacctctggc gtgaaagaga ttcgcgaggc gatcgagcgc gcccggcaaa
    59221 accgcaatgc aggtcgccgc actattcttt ttgttgacga agttcaccgt ttcaacaaaa
    59281 gccagcagga tgcatttctg ccacatattg aagacggcac catcactttt attggcgcaa
    59341 ccactgaaaa cccgtcgttt gagcttaatt cggcactgct ttcccgtgcc cgtgtttatc
    59401 tgttgaaatc cctgagtaca gaggatattg agcaagtact aactcaggcg atggaagaca
    59461 aaacccgtgg ctatggtggt caggatattg ttctgccaga tgatacacga cgtgccattg
    59521 cagaactggt gaatggcgac gcgcgccggg cgttaaatac gctggaaatg atggcggata
    59581 tggccgaagt ctatgatagc ggtaagcggg tcctgaagcc tgaattactg accgaaatcg
    59641 ccggtgaacg tagcgcccgc tttgataaca aaggcgatcg cttttacgat ctaatttccg
    59701 cactgcataa gtcggtacgt ggtagcgcac ccgatgcggc gctgtactgg tatgcgcgaa
    59761 ttattaccgc tggtggcgat ccgttatatg tcgcgcgtcg ctgtctggcg attgcgtctg
    59821 aagacgtcgg taatgccgat ccacgggcga tgcaggtggc aattgcggcc tgggattgct
    59881 ttactcgcgt tggcccggcg gaaggtgaac gcgccattgc tcaggcgatt gtttacctgg
    59941 cctgcgcgcc aaaaagcaac gctgtctaca ctgcgtttaa agccgcgctg gccgatgctc
    60001 gcgaacgccc ggattatgac gtgccggttc atttgcgtaa tgcgccgacg aaattaatga
    60061 aggaaatggg ctacgggcag gaatatcgtt acgctcatga tgaagcaaac gcttatgctg
    60121 ccggtgaggt ttacttcccg ccggaaatag cacaaacacg ctattatttc ccgacaaaca
    60181 ggggccttga aggcaagatt ggcgaaaagc tcgcctggct ggctgaacag gatcaaaata
    60241 gccccataaa acgctaccgt taatgttatc gttgcggtaa tgttgttact gtatccctgt
    60301 ggtcgcaggc tgtggccaca tctcccattt aattcgataa gcacaggata agcatgctcg
    60361 atcccaatct gctgcgtaat gagccagacg cagtcgctga aaaactggca cgccggggct
    60421 ttaagctgga tgtagataag ctgggcgctc ttgaagagcg tcgtaaagta ttgcaggtca
    60481 aaacggaaaa cctgcaagcg gagcgtaact cccgatcgaa atccattggc caggcgaaag
    60541 cgcgcgggga agatatcgag cctttacgtc tggaagtgaa caaactgggc gaagagctgg
    60601 atgcagcaaa agccgagcta gatgctttac aggctgaaat tcgcgatatc gcgctgacca
    60661 tccctaacct gcctgcagat gaagtgccgg taggtaaaga cgaaaatgac aacgttgaag
    60721 tcagccgctg gggtaccccg cgtgagtttg actttgaagt tcgtgaccac gtgacgctgg
    60781 gtgaaatgca ctctggcctc gactttgcag ctgcagttaa gctgactggt tcccgctttg
    60841 tggtaatgaa agggcagatt gctcgcatgc accgcgcact gtcgcagttt atgctggatc
    60901 tgcataccga acagcatggc tacagtgaga actatgttcc gtacctggtt aaccaggaca
    60961 cgctgtacgg tacgggtcag ctgccgaaat ttgctggcga tctgttccat actcgtccgc
    61021 tggaagaaga agcagacacc agtaactatg cgctgatccc aacggcagaa gttccgctga
    61081 ctaacctggt gcgcggtgaa atcatcgatg aagatgatct gccaattaag atgaccgccc
    61141 acaccccatg cttccgttct gaagccggtt catatggtcg tgacacccgt ggtctgatcc
    61201 gtatgcacca gttcgacaaa gttgaaatgg tgcagatcgt gcgcccagaa gactcaatgg
    61261 cggcgctgga agagatgact ggtcatgcag aaaaagtcct gcagttgctg ggcctgccgt
    61321 accgtaaaat catcctttgc actggcgaca tgggctttgg cgcttgcaaa acttacgacc
    61381 tggaagtatg gatcccggca cagaacacct accgtgagat ctcttcctgc tccaacgttt
    61441 gggatttcca ggcacgtcgt atgcaggcac gttgccgcag caagtcggac aagaaaaccc
    61501 gtctggttca taccctgaac ggttctggtc tggctgttgg tcgtacgctg gttgcagtaa
    61561 tggaaaacta tcagcaggct gatggtcgta ttgaagtacc agaagttctg cgtccgtata
    61621 tgaacggact ggaatatatt ggctaatacc caatttttct gaatctaaaa agcgccttta
    61681 gggcgctttt tttgtctccc tttgataccg aacaataatt actcctcact tacacgtaat
    61741 actactttcg agtgaaaatc tacctatctc tttgattttc aaattattcg atgtatacaa
    61801 gcctatatag cgagctgcta tagaaataat tacacaatac ggtttgttac tggaatcaat
    61861 cgtgatcaag cttgagtgag ccattatgaa aacgaaaatc cctgatgcgg tattggctgc
    61921 tgaggagagt cgccgtggtt tggtaaaaac gacagcgatc ggcggcctgg caatggccag
    61981 cagcgcatta acattacctt ttagtcggat tgcgcacgct gtcgatagcg ccattccaac
    62041 aaaatcagac gaaaaggtta tctggagcgc ctgtacagtt aactgtggta gtcgctgccc
    62101 gctacgtatg cacgtcgtgg acggtgaaat caaatatgtc gaaacggaca ataccggcga
    62161 tgacaattac gacggcctgc actaggttcg cgcctgcctg cgtgggcgtt ccatgcgtcg
    62221 ccgtgtctac aatccggacc gcctgaaata tccgatgaaa cgagtcgggg cgcgcggtga
    62281 aggcaaattc gagcgcatta gctgggaaga agcctacgac atcatcgcga ccaatatgca
    62341 gcgcctgatc aaagagtacg gcaacgagtc tatctatctg aactatggca ccggtacgct
    62401 gggcggcacc atgacccgct cctggccgcc gggaaatacc ctggtcgcgc gcctgatgaa
    62461 ctgctgcggc ggttatctta accattacgg tgactactct tccgcgcaaa ttgcggaagg
    62521 tttgaaccat acctacggcg gctgggcaga tggtaacagc ccgtcggata tcgaaaacag
    62581 taaattggta gtgctgtttg gtaataaccc aggcgaaacg cgaatgagtg gcggtggggt
    62641 gacttactat cttgaacagg cacgccagaa atctaatgct cgcataatca tcatcgatcc
    62701 gcgctatacc gacaccggtg ccgggcgcga agatgagtgg atccctattc gtccaggaac
    62761 agatgccgca ctggttaacg gtctggcgta cgtcatgatc actgaaaacc tggtggatca
    62821 ggcattcctc gataaatatt gcgttggcta tgacgagaaa accctgcccg ccagtgcgcc
    62881 gaaaaatggt cattataaag cttatattct gggcgaaggg ccagatggcg tggctaaaac
    62941 accggaatag gcctcgcaaa tcaccggtgt tccggcagac aaaatcatca aactggctcg
    63001 tgaaatcggc agtaccaaac cggcgtttat cagccaggga tggggcccgc agcgtcacgc
    63061 taacggtgaa atcgcaaccc gtgctatctc gatgctggcg attctgaccg gtaacgttgg
    63121 tattaatgga gggaacagcg gcgcgcgtga aggttcatac agcttaccgt ttgtccgtat
    63181 gccgaccttg gaaaacccga tccagaccag catttcgatg tttatgtgga ccgatgccat
    63241 tgaacgtggc ccggaaatga cggcgctgcg tgatggtgtg cgcgggaaag ataagctgga
    63301 tgtgccgatc aaaatgatct ggaactatgc cggtaactgc ctgattaacc agcattctga
    63361 aatcaaccgt acccatgaaa tcctccagga tgataagaag tgcgagctga ttgtggttat
    63421 cgactgccac atgacctcat cggcgaaata tgctgacatc ctgctgcctg actgcaccgc
    63481 ttccgaacag atggactttg cactggatgc atcctgcggg aatatgtctt acgtgatttt
    63541 caacgatcag gtgattaaac cgcgctttga atgtaagacc atctatgaaa tgaccagcga
    63601 actggcaaaa cgtcttggcg ttgagcaaca gtttactgaa ggccgtaccc aggaagagtg
    63661 gatgcggcat ctgtatgccc agtcgcggga agcgattcct gaactgccaa cgtttgaaga
    63721 gttccgcaag caggggatct ttaaaaagcg cgacccacaa gggcatcacg ttgcttataa
    63781 agccttccgt gaagatccgc aggcaaaccc actgactacg ccatcgggca aaattgagat
    63841 ttattcgcag gcgctggctg acattgccgc tacctgggaa ttgccagaag gcgatgtgat
    63901 cgatccactg ccgatctaca cgccgggctt tgaaagttat caggatccgc tgaacaaaca
    63961 gtatccgctg cagcttacag gtttccacta taaatctcgc gttcactcaa cttacggcaa
    64021 cgttgatgtg ctgaaagcgg cttgccgtca ggaaatgtgg atcaacccgc ttgatgccca
    64081 aaaacgcggt atccacaacg gcgataaagt caggatcttt aacgatcgtg gtgaggttca
    64141 tattgaggcg aaagtgacgc cacgaatgat gccgggcgtg gtcgcactgg gtgaaggcgc
    64201 ctggtatgac ccggatgcaa aacgtgtcga taagggcggt tgtatcaacg tactgaccac
    64261 tcaacgtccg tctcctctcg ctaaggggaa tccgtcacat acaaaccttg ttcaggttga
    64321 aaaggtgtaa ggagtaaccg atgacaaccc agtatggatt ttttattgat tccagccgtt
    64381 gcaccggttg caaaacctgc gagctggctt gtaaagacta caaagatttg acgccagaag
    64441 tcagtttccg ccgcatttat gaatatgctg gcggcgactg gcaggaagat aacggtgtct
    64501 ggcaccagaa cgtgtttgcc tactatctgt cgatttcgtg caaccactgc gaagatccgg
    64561 cttgtactaa agtctgcccg agcggtgcga tgcataaacg cgaagatggt tttgttgtgg
    64621 tcgatgaaga tgtatgcatt ggctgccgtt actgtcatat ggcttgcccg tatggcgcac
    64681 cgcaatataa cgaaacgaaa ggccatatga ccaaatgcga tggttgttat gaccgtgttg
    64741 cggagggtaa aaagccgatc tgtgttgaat cttgcccgct gcgggcgctg gatttcggcc
    64801 ctatcgacga gctgcgtaaa aaacatggcg atctggcggc cgttgcgccg ttgccgcgag
    64861 ctcactttac caaaccgaat attgtgatca aacccaatgc caatagccgc ccgacagggg
    64921 ataccactgg ctatctggca aacccgaagg aggtgtgaga tgggaagtgg atggcatgaa
    64981 tggccgctga tgatcttcac ggtcttcggg caatgtgtgg cgggtggttt tatcgttctg
    65041 gctttggcgc tgctcaaagg cgacctgcga gcagaagccc agcagcgtgt tatcgcctgc
    65101 atgtttggct tatgggtact gatgggcatt ggctttatcg cctctatgct ccatcttggt
    65161 tcaccgatgc gcgcctttaa ctcgctcaac cgggtagggg cttcagcact cagtaacgaa
    65221 atcgccagcg gttcgatctt ttttgctgta ggcggcatcg gctggttgct ggcaatgctg
    65281 aaaaagctgt caccggcatt gcgtacgcta tggctggtgg tgacgatggt tcttggcgtc
    65341 gtctttgtct ggatgatggt gcgtgtgtat aacagcattg ataccgttcc gacctggtac
    65401 agcatctgga cgccgatggg cttcttcctg acgatgttca tgggcggccc gttactgggt
    65461 tacctgttgc tgagtctggc tggcgtcgat ggctgggcga tgcgtctgct gccagccatt
    65521 tctgtactgg cactggtggt gagtggcgtg atgtcagtga tgcagggcac agagctggca
    65581 actattcata gttctgtgca gcaggccgca gcgctggtgc cggactatgg tgcgctgatg
    65641 tcctggcgga tcgtgctttt ggccgttgcc ctgtgcttgt ggattgcacc acagctaaaa
    65701 ggttatcagc ctgcggttcc gctactatcc gtctcattca ttctgcttct ggcaggggaa
    65761 ttaatcggtc gcggcgtatt ctatggtttg catatgaccg tggggatggc cgtcgcaagc
    65821 taatcagtaa caaccggggt ttcggccccg gttctctttt atttctgctt cgttaacgtg
    65881 tcataactgg tcatcaaatt acgataatca ggaatatggt tagagaacaa cgtcgccagt
    65941 ccttcaatat cattacgcca gtcacggtgc agctcgcaag ccacgccgaa ccatgtcatc
    66001 aattgcgcac cagcttgcga catgcgatcc cacgccgaat gccgggtaat ttcattaaag
    66061 gtccctgatg catcagtcac gacaaagaca tcaaaccctt cttcaatggc tgaaagcgcc
    66121 gggaatgcca cgcaaacttc ggttaccaca ccggcaataa ttaactgttt tttacctgtc
    66181 gctttgactg cttttacaaa atcttcgtta tcccaggcgt taatattgcc cgggcgagca
    66241 atgtaaggtg catcggggaa ttgtgctttc agttctagaa ctaatgggcc gttaggaccg
    66301 gtttcaaaac tggtggtaag aatagttggt aagttgaaat acttagccag gtcacccagc
    66361 gccagcacgt tatttttaaa cttatcgggt tcgatatccc gtacaaggga aagtaaacca
    66421 gcctggtgat caacaagcaa aacggcagca tcatttttat caagacgaac atacggtttg
    66481 gtcatcctct tctcctttcg agagtcagtg gtacgtctga aatcggtcag acgcgatgca
    66541 ttgctctgaa agcatagacg ggaaatatga gtttgctgtg accatgaaat ttttcgactg
    66601 aacgaagcgt atgaaatttg tgttagatca ataaaaacaa tcagatgaga ttaatcatct
    66661 cttcagcatt agcgttatga atgagtaata taaatcgata ataccgccgc tgcgatatgc
    66721 gcgtattttc acccattgac aatgtttttg gcggtggcat gatgcgcatg aaatttgaac
    66781 ttcctcacgg ttttaattca tgtccacgta tacccggcct gtcatgcttt tgctgtctgg
    66841 cctgcttttg ttgactctgg cgatcgcggt gttaaataca ctcgtgccgc tttggctcgc
    66901 ccaggaacac atgtccacat ggcaggtagg cgttgtcagc tcatcctatt ttaccggcaa
    66961 ccttgtcggt acattgctga cagggtatgt cattaaacgc attggcttta accgcagcta
    67021 ttatctgtcc tccttcattt ttgctgctgg ctgtgccggc cttggcctga tgattggttt
    67081 ctggagctgg ttggcgtggc gttttgtcgc gggcgtcggc tgtgccatga tttgggtggt
    67141 tgttgagagc gcgctgatgt gcagtgggac gtcacgtaac cgtgggcgtt tgcttgctgc
    67201 gtatatgatg gtttattacg tgggaacgtt tttaggccag ttactggtca gcaaagtctc
    67261 aaccgagctg atgagcgtat tgccgtgggt tacaggtttg acattggcag ggatcttacc
    67321 gctgttgttt acgcgtgtcc tgaatcagca ggctgaaaac catgattcga cgtcaattac
    67381 ggcaatgcta aaactccgtc aggcgcggct tggcgtgaat ggctgcatta tctcaggaag
    67441 cgttctggga tctctatatg gcctgatgcc tctgtacctc aatcacaaag gggtgagcaa
    67501 tgccagtatt ggtttctgga tggcggtact ggtcagtgcg ggtatcctcg gacaatggcc
    67561 gattggacgt ctggcggata agtttggtcg actgctggtt ttgcgtgttc aggtctttgt
    67621 cgtcattctc ggcagtattg cgatgcttag ccaggcggcg atggccccag cgttattcat
    67681 cctcggtgcc gctggcttta cgctatatcc ggtggcgatg gcctgggctt gtgagaaagt
    67741 tgaacatcat caactggtgg cgatgaacca agccttactg ttgagctata ccgtgggaag
    67801 tctgcttggc ccgtcattta ccgctatgct aatgcagaat ttctccgata atttattgtt
    67861 tatcatgatc gccagcgtat cgtttatcta tttgctgatg ctgcttcgca acgccggtca
    67921 tacgccgaaa cccgttgctc acgtgtaaat gaattcaagc aaagtgtgaa cttactgttt
    67981 cacactctgc ttttttgttt cttctatctg acttgctttt ttccaaattt tattcgttta
    68041 aaaataaaat gtgcagcagg ttataatttt gcatttcgct atttccgcac ttcttatttg
    68101 ccgcgcataa tccctcgttt tacagatgcc cctttaattt tggcgaagga tttgtctatg
    68161 gctgggaatg ttcaggaagt aagcgcccca tca
//
LOCUS       DAETPZ010000008        67095 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.008, whole genome shotgun sequence.
ACCESSION   DAETPZ010000008 DAETPZ010000000
VERSION     DAETPZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.008"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(64..1068)
                     /gene="cra"
                     /locus_tag="K0W59_000623"
     CDS             complement(64..1068)
                     /gene="cra"
                     /locus_tag="K0W59_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HBI3021782.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1150..1236)
                     /locus_tag="K0W59_000624"
     CDS             complement(1150..1236)
                     /locus_tag="K0W59_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HBI3021783.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="K0W59_000625"
     CDS             complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="K0W59_000625"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBI3021784.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="K0W59_000626"
     CDS             complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="K0W59_000626"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HBI3021785.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3585..3629
                     /locus_tag="K0W59_000627"
     CDS             3585..3629
                     /locus_tag="K0W59_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3021786.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="K0W59_000628"
     CDS             complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="K0W59_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HBI3021787.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5389..5475
                     /gene="leuL"
                     /locus_tag="K0W59_000629"
     CDS             5389..5475
                     /gene="leuL"
                     /locus_tag="K0W59_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HBI3021788.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5568..7139
                     /gene="leuA"
                     /locus_tag="K0W59_000630"
     CDS             5568..7139
                     /gene="leuA"
                     /locus_tag="K0W59_000630"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBI3021789.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7139..8230
                     /gene="leuB"
                     /locus_tag="K0W59_000631"
     CDS             7139..8230
                     /gene="leuB"
                     /locus_tag="K0W59_000631"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBI3021790.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8233..9633
                     /gene="leuC"
                     /locus_tag="K0W59_000632"
     CDS             8233..9633
                     /gene="leuC"
                     /locus_tag="K0W59_000632"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBI3021791.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKSGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9644..10249
                     /gene="leuD"
                     /locus_tag="K0W59_000633"
     CDS             9644..10249
                     /gene="leuD"
                     /locus_tag="K0W59_000633"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBI3021792.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10298..11476)
                     /gene="setA"
                     /locus_tag="K0W59_000634"
     CDS             complement(10298..11476)
                     /gene="setA"
                     /locus_tag="K0W59_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HBI3021793.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11578..11709)
                     /gene="sgrT"
                     /locus_tag="K0W59_000635"
     CDS             complement(11578..11709)
                     /gene="sgrT"
                     /locus_tag="K0W59_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HBI3021794.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11798..13453
                     /gene="sgrR"
                     /locus_tag="K0W59_000636"
     CDS             11798..13453
                     /gene="sgrR"
                     /locus_tag="K0W59_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HBI3021795.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGDLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13487..13586
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13617..14600
                     /gene="thiB"
                     /locus_tag="K0W59_000637"
     CDS             13617..14600
                     /gene="thiB"
                     /locus_tag="K0W59_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HBI3021796.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14576..16186
                     /gene="thiP"
                     /locus_tag="K0W59_000638"
     CDS             14576..16186
                     /gene="thiP"
                     /locus_tag="K0W59_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HBI3021797.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16170..16868
                     /gene="thiQ"
                     /locus_tag="K0W59_000639"
     CDS             16170..16868
                     /gene="thiQ"
                     /locus_tag="K0W59_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HBI3021798.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIVWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16982..17746)
                     /locus_tag="K0W59_000640"
     CDS             complement(16982..17746)
                     /locus_tag="K0W59_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI3021799.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17832..18710)
                     /gene="araC"
                     /locus_tag="K0W59_000641"
     CDS             complement(17832..18710)
                     /gene="araC"
                     /locus_tag="K0W59_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HBI3021800.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19049..20748
                     /gene="araB"
                     /locus_tag="K0W59_000642"
                     /pseudo
     CDS             19049..20748
                     /gene="araB"
                     /locus_tag="K0W59_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
     gene            20759..22261
                     /gene="araA"
                     /locus_tag="K0W59_000643"
     CDS             20759..22261
                     /gene="araA"
                     /locus_tag="K0W59_000643"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBI3021801.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22461..23156
                     /gene="araD"
                     /locus_tag="K0W59_000644"
     CDS             22461..23156
                     /gene="araD"
                     /locus_tag="K0W59_000644"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBI3021802.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSDVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23231..25582
                     /gene="polB"
                     /locus_tag="K0W59_000645"
     CDS             23231..25582
                     /gene="polB"
                     /locus_tag="K0W59_000645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HBI3021803.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWVRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25747..28653
                     /gene="rapA"
                     /locus_tag="K0W59_000646"
     CDS             25747..28653
                     /gene="rapA"
                     /locus_tag="K0W59_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HBI3021804.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNTVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28665..29324
                     /gene="rluA"
                     /locus_tag="K0W59_000647"
     CDS             28665..29324
                     /gene="rluA"
                     /locus_tag="K0W59_000647"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HBI3021805.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARAVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="K0W59_000648"
     CDS             complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="K0W59_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HBI3021806.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30511..32865
                     /gene="lptD"
                     /locus_tag="K0W59_000649"
     CDS             30511..32865
                     /gene="lptD"
                     /locus_tag="K0W59_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HBI3021807.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTYVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32918..34204
                     /gene="surA"
                     /locus_tag="K0W59_000650"
     CDS             32918..34204
                     /gene="surA"
                     /locus_tag="K0W59_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HBI3021808.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKLSEEAASWMQEQRASAYVKILSN"
     gene            34204..35193
                     /gene="pdxA"
                     /locus_tag="K0W59_000651"
     CDS             34204..35193
                     /gene="pdxA"
                     /locus_tag="K0W59_000651"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HBI3021809.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGITEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35190..36011
                     /gene="rsmA"
                     /locus_tag="K0W59_000652"
     CDS             35190..36011
                     /gene="rsmA"
                     /locus_tag="K0W59_000652"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBI3021810.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36014..36391
                     /gene="apaG"
                     /locus_tag="K0W59_000653"
     CDS             36014..36391
                     /gene="apaG"
                     /locus_tag="K0W59_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HBI3021811.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36398..37240
                     /gene="apaH"
                     /locus_tag="K0W59_000654"
     CDS             36398..37240
                     /gene="apaH"
                     /locus_tag="K0W59_000654"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HBI3021812.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37318..37797)
                     /gene="folA"
                     /locus_tag="K0W59_000655"
     CDS             complement(37318..37797)
                     /gene="folA"
                     /locus_tag="K0W59_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HBI3021813.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="K0W59_000656"
     CDS             complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="K0W59_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HBI3021814.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="K0W59_000657"
     CDS             complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="K0W59_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HBI3021815.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40421..41815)
                     /locus_tag="K0W59_000658"
     CDS             complement(40421..41815)
                     /locus_tag="K0W59_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3021816.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="K0W59_000659"
     CDS             complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="K0W59_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HBI3021817.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42158..43443)
                     /locus_tag="K0W59_000660"
                     /pseudo
     CDS             complement(42158..43443)
                     /locus_tag="K0W59_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(43494..44435)
                     /locus_tag="K0W59_000661"
     CDS             complement(43494..44435)
                     /locus_tag="K0W59_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBI3021818.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQTRQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVRISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(44450..45220)
                     /locus_tag="K0W59_000662"
     CDS             complement(44450..45220)
                     /locus_tag="K0W59_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HBI3021819.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45691..47205
                     /gene="caiT"
                     /locus_tag="K0W59_000663"
     CDS             45691..47205
                     /gene="caiT"
                     /locus_tag="K0W59_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HBI3021820.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47236..48378
                     /gene="caiA"
                     /locus_tag="K0W59_000664"
     CDS             47236..48378
                     /gene="caiA"
                     /locus_tag="K0W59_000664"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HBI3021821.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48507..49724
                     /gene="caiB"
                     /locus_tag="K0W59_000665"
     CDS             48507..49724
                     /gene="caiB"
                     /locus_tag="K0W59_000665"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HBI3021822.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49798..51351
                     /gene="caiC"
                     /locus_tag="K0W59_000666"
     CDS             49798..51351
                     /gene="caiC"
                     /locus_tag="K0W59_000666"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HBI3021823.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51460..52245
                     /gene="caiD"
                     /locus_tag="K0W59_000667"
     CDS             51460..52245
                     /gene="caiD"
                     /locus_tag="K0W59_000667"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HBI3021824.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52251..52841
                     /gene="caiE"
                     /locus_tag="K0W59_000668"
     CDS             52251..52841
                     /gene="caiE"
                     /locus_tag="K0W59_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HBI3021825.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52927..53322)
                     /gene="caiF"
                     /locus_tag="K0W59_000669"
     CDS             complement(52927..53322)
                     /gene="caiF"
                     /locus_tag="K0W59_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HBI3021826.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53580..56801)
                     /gene="carB"
                     /locus_tag="K0W59_000670"
     CDS             complement(53580..56801)
                     /gene="carB"
                     /locus_tag="K0W59_000670"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBI3021827.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56819..57967)
                     /gene="carA"
                     /locus_tag="K0W59_000671"
     CDS             complement(56819..57967)
                     /gene="carA"
                     /locus_tag="K0W59_000671"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBI3021828.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58423..59244)
                     /gene="dapB"
                     /locus_tag="K0W59_000672"
     CDS             complement(58423..59244)
                     /gene="dapB"
                     /locus_tag="K0W59_000672"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBI3021829.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59411..60325)
                     /gene="rihC"
                     /locus_tag="K0W59_000673"
     CDS             complement(59411..60325)
                     /gene="rihC"
                     /locus_tag="K0W59_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HBI3021830.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60391..61341)
                     /gene="ispH"
                     /locus_tag="K0W59_000674"
                     /gene_synonym="lytB"
     CDS             complement(60391..61341)
                     /gene="ispH"
                     /locus_tag="K0W59_000674"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBI3021831.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61343..61792)
                     /gene="fkpB"
                     /locus_tag="K0W59_000675"
     CDS             complement(61343..61792)
                     /gene="fkpB"
                     /locus_tag="K0W59_000675"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBI3021832.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61879..62373)
                     /gene="lspA"
                     /locus_tag="K0W59_000676"
     CDS             complement(61879..62373)
                     /gene="lspA"
                     /locus_tag="K0W59_000676"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBI3021833.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62373..65189)
                     /gene="ileS"
                     /locus_tag="K0W59_000677"
     CDS             complement(62373..65189)
                     /gene="ileS"
                     /locus_tag="K0W59_000677"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBI3021834.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAAILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65232..66173)
                     /gene="ribF"
                     /locus_tag="K0W59_000678"
     CDS             complement(65232..66173)
                     /gene="ribF"
                     /locus_tag="K0W59_000678"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBI3021835.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66181..66399)
                     /locus_tag="K0W59_000679"
     CDS             complement(66181..66399)
                     /locus_tag="K0W59_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HBI3021836.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66502..66765
                     /gene="rpsT"
                     /locus_tag="K0W59_000680"
     CDS             66502..66765
                     /gene="rpsT"
                     /locus_tag="K0W59_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBI3021837.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16279 a        18257 c        17273 g        15286 t
ORIGIN      
        1 tgtccagtcc cgtactctac gcgccagagg gaaattcacc tggcgcgtat ttttgttcgc
       61 ggcttagcta cggctgagca cgccgcggcg atagagatta cgtttaatgc gcgttaaacc
      121 aggttttggc ttacgcggtt cgtccaggct tgccaggaca atctccagca cacgctctgc
      181 gacatcgcgg tgacgttgag ccactgccag caccggacac tgtaagaagt cgagcagttc
      241 gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt ttgccgtcgc gacgcaacgt
      301 gacatccatc actccttgca acaacgcaaa cgacgttgtg aacagcgcct gcggcatcgg
      361 atgcgtttcc agccattttt cgaataactg ggcagccgcc tcccgctcat agctgttggc
      421 atacaggaaa tgcacttcgc gcggatcatc tttccaagca gtacggaaac cttgttcacg
      481 caggaagctg acagaaagct ccggcagcgc gccaagataa agcaccgtct cggcgggaaa
      541 cttacgtaac tcttccgcca gcatttcggc atcatcctga tcggcaccaa ccacgctggt
      601 gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc gggaacgggt cgttagccca
      661 gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa acaataatgg catcaacctg
      721 acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtct ggctgatctt ctgagcaggc
      781 aatcagcagt tgataacccc gttgccgcgc ctggcgttca agatagttag cgatgcgggt
      841 atagctggtg ttctccagat cggggatcac aagaccaata gaacgtgtgc gtccagcacg
      901 aagcccagct gccacggcgt tcgggtggta attgtgctca cgcaccacag ccatgacttt
      961 ttcaacggtt ttgtcgctca cacggtattg cttcgctttg ccgttaataa catagcttgc
     1021 agtggtccgc gacactcccg ccagccgagc gatttcatcc agtttcacaa ttgccccttg
     1081 cgtaaaatgt aaaaaccata gccattgtac tggtatgggt taaattcatt aacatctaag
     1141 cgcagaaaat taactgcggc aactgctttt attccaggtt cccactgatt tcgcaaaaaa
     1201 agcccaacgt gacatgttgg gctgcaaatt gcgcactgag atcattctca acgcattatt
     1261 ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa cttccacaat tttcgccaca
     1321 tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg tgcctgctaa ttgaacggta
     1381 taaagcgagg gggtgacatc gataatttgc ccacggaata tttccgtatt acgtttcact
     1441 tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca tgatttcccg ctcaacatgc
     1501 gcgccctgcc ccaactcact cacgcgcaag acatcgacca gcttgtgtaa ttgcttttcg
     1561 atctgctcaa gtactttttc atcgcccacg gtctggatgg tcatacgcga taatgtcgga
     1621 tcgtcggttg gcgcaacggt caggctttca atgttgtagc ctcgctggga aaaaaggcca
     1681 atcacgcggg ataacgcgcc tgattcattt tcgagtaaga ctgataatat ccggcgcata
     1741 atcaggttct ctccgttttg cttaaccaca tttcatccat tccgcccccg cgaatctgca
     1801 tcgggtagac gtgctcactg ccatcgacgg taacatcaac aaacaccagg cgattattgc
     1861 gcacctgttc cagcgcctcg ctaagtttgc tttccagctc atgcggatga gagatctgga
     1921 tcccgacgtg accataggct tccgccagac ggacgaaatc gggtagtgat tgcatataag
     1981 attgtgaatg acggccggaa tagatcatgt cctgccactg tttcaccatc cccagatagc
     2041 ggttattgag attcaccacc agtacgggca actcgtattg caacgcggta gacagttcct
     2101 ggatgttcat ctgaatactg ccgtcgccag tgacgcaaac cacggtttct tctggcaacg
     2161 ccattttgac gcccagtgcc gcaggtaaac caaaacccat cgtgccgaga ccgccggaat
     2221 tgatccagcg acgcggtttg tcgaatggat aataaagcgc agcaaacatc tggtgctgcc
     2281 cgacatcgga cgtcacgtaa gcgtccccat tagtcaaccg ccaaagagtc tcgatcaccg
     2341 cctgcggttt aatcttttca ctgtgagtgt catatttcag gcactgaaga gcgcgccact
     2401 gttcaatctg ctgccaccag tcgcggatct catccagcgg ttgatgggcg gattcttgcg
     2461 acaggagttc aagcatttgt tcgaggacct ggcgagcatc ccccacaatc gggatatccg
     2521 cagtcacggt tttagaaatg gaagtaggat caatatcgat atgcagaaca gtggcatttg
     2581 ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa tcgtaccccg acggcgaaaa
     2641 tcacatccgc gttatgcatc gtcatattgg cttcgtaggt accgtgcatt cccagcatgc
     2701 ccagtgcctg acgatgcgtt gccggaaacg cccccagccc catcaatgag gaaacaacgg
     2761 gcagattcaa cgcctccacc gtttctttca actgctgatg gcagcccgcc atgattgccc
     2821 caccgcctac gtagacaacc ggtttttttg ccgctaccag cgtttgcaga gcacgcttaa
     2881 tttgcccttt atgtccggta gtagtgggat tgtaagaacg catactgacc gactcagggc
     2941 agacataggg taatttgttc gccggattaa gaatatcttt cggtaaatca acgaccactg
     3001 gtcctgggcg accacttgcc gccagccaga aagccttttt cagcacctgc ggaatgtctt
     3061 ccgtttgctt aaccagaaaa ctgtgtttaa ccaccggtcg cgaaatcccc accatgtcgc
     3121 actcctgaaa agcatcgtaa cctatcaacg aggtcgctac ctgcccggaa aggacaacta
     3181 atggaatgga atccatataa gcggtggcga tgccggtaat cgcattggtc gcccctggac
     3241 ccgacgttac cagcacgacg ccgacttccc cggtcgcacg cgccaggcca tcggccatat
     3301 gcaccgccgc ctgctcatga cgaactaata catgatcaat accacccacg gtatgcaatg
     3361 catcgtaaat atcaaggact gcgcctccgg gataaccgaa tacttgttta acgccctgat
     3421 cgataagcga tcggacgacc atctcggctc cagacaacat ctccatggcc tgcctcactg
     3481 tttgacggaa aaaatgtgta aaacacatta accgtccgat cttgcttaag caattggcag
     3541 ccagagacat aaatgcaggg taataagaat aaacaggagg aaaggtgaaa agaaacgata
     3601 aaatcctcca ttacgaagga ttaaattgat aataaatcgg ctgaatccca caacttacag
     3661 caatttctct atttttttaa ttaatcctaa aatagagagt ccacacattc agaataaaat
     3721 tctacattca ataaaaaatg cagaataaac cagacattca tgtctggcct attctgcatt
     3781 cagttagcgt ttgcaaattg agactaattg ttcttccatc cactgatggc ctttatcgcg
     3841 cccggcagct tcatgccaag agagataaca ggttctgctg ttttgtttta acggcagcgg
     3901 taatacctgt aattctaagg attcagcgaa ctcttcagcc agccaacgcg gcgcaatagc
     3961 gaccaaatgc gtttgcgaca ccacgctaag tacgctcatc attgccatgc cctgatacgc
     4021 gatactggct tgcttatcta ccgtgtcata ccaaggttga ctaaatgacg cgaaacgatc
     4081 gagcgaaacc gccgcatgtt gttcgttata aacatcatgt ttcagtaacg ggcctttaat
     4141 tgttggatga tttttgctgg ctaccagcac catttcatct ttaaataatg gcacgctggt
     4201 aaattcagga cgatggaagt cttcataact aatcacaaac tccgtttcct gataacgcag
     4261 ctgatgttca gtgttctgat ttaatgaaga cttgaacata acatgtatat ttggcgcaat
     4321 ctgctcaatg tgattataaa tctgcgaggt cagaatgctg tctaacgggc tgcaaacaca
     4381 aagatgaaat acacgttcac tgctcgcggg ttcaaaacct gaaccaggca attcattttg
     4441 tactagttgc aatgcctggc gaactgaacc aaaaagttga aatgcgcgag cagtcggttg
     4501 aataccacgg ccataacgaa caaaaagctc gtcattaaac atcaccttca ggcgtgcaac
     4561 agcgttactg accgcaggtt gcgacatacc cagaacatga gcggcacggg taatgttttg
     4621 ctcctgcatc acggcatcga aaacggttaa taagttgaga tcgaccatgc gtagctgtgg
     4681 cttgcttaac tccgccgtct ctgaatgatc tgtttgtacc tctggcatac ttaactccac
     4741 tgtcacactt aactcccttt cccttattgg aatgcagaga aataatcctg aagaatatga
     4801 tttatcatgc atataaaata agaaaaagca aaatgagtaa aattcggaaa acataaagat
     4861 acgggttctg ataaaaacag aatccattgc cgtggaatga gtcatttacc acagcataat
     4921 aatccataat gtttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattattt tatatattta atgcattaat
     5041 tcttaacatt aattgatcaa taatattcac caaatcaata tcaaaaaaaa atcgcaaaac
     5101 atataattca atacaaatca tcaggatagg ttttgcaacg cgtgcatttt gtcccctttt
     5161 tcctcgttga ttagatgcaa aaatttatgc tgaaatatgt caaccgatga aaagcgtcgg
     5221 tagttaagca gaaattaata tcgcttactt taaccaccgt agcacaatta gctaatttta
     5281 cggatgcaga actcacgctg gcgggacgtt tttattgcgt cagggttgac atccgttttt
     5341 gtatccagta actctaaaag catatcgcat tcatctggag ctgatttaat gactcacatc
     5401 gttcgcttta tcggtctact actactaaac gcatcttctt tgcgcggtag acgagtgagc
     5461 ggcatccagc attaagccag cacgcagtca aacaaaaaac ccgcgccatt gcgcgggttt
     5521 ttttatgccc gaagcgaggc gctctaaaag agacaaggac ccaaaccatg agccagcaag
     5581 tcattatttt cgataccaca ttgcgcgacg gtgaacaggc gttacaggca agcttgagtg
     5641 tgaaagaaaa actgcaaatt gcgctggccc ttgagcgtat gggtgttgac gtgatggaag
     5701 tcggtttccc cgtctcttcg ccgggcgatt ttgaatcggt gcaaaccatc gcccgccagg
     5761 ttaaaaacag ccgcgtatgt gcgttagctc gctgcgtgga aaaagatatc gacgtggcgg
     5821 ccgaatccct gaaagtcgcc gaagccttcc gtattcatac ctttattgct acctccccaa
     5881 tgcacatcgc caccaagctg cgcagcaccc tggacgaggt gatcgaacgc gctatctata
     5941 tggtgaaacg cgcccgtaat tacaccgatg atgttgaatt ttcttgcgaa gatgccgggc
     6001 gtacacccat tgccgatctg gcgcgagtgg tcgaagcggc gattaatgcc ggtgccacca
     6061 ccatcaacat tccggacacc gtgggctaca ccatgccgtt tgagttcgcc ggaatcatca
     6121 gcggcctgta tgaacgcgta cctaacatcg acaaagccat tatttccgta catacccacg
     6181 acgatttagg cctggcggtc ggcaactcac tggcggcggt acatgccggt gcacgccagg
     6241 tggaaggtgc aatgaacggg atcggcgagc gtgccggtaa ctgttcgctg gaagaagtca
     6301 tcatggcgat taaagttcgt aaggatattc tcaacgtcca caccgccatt aatcaccagg
     6361 agatatggcg caccagccag ttagttagcc agatttgtaa tatgccgatc ccggcaaaca
     6421 aagccattgt tggcagcggc gcattcgctc actcctccgg tatccaccag gatggcgtgc
     6481 tgaaaaaccg cgaaaactac gaaatcatga caccagaatc tattggtctg aaccaaatcc
     6541 agctgaatct gacctctcgt tcggggcgtg cggcggtgaa acatcgcatg gatgagatgg
     6601 ggtataaaga aagtgaatat aatttagaca atttgtacga tgctttcctg aagctggcgg
     6661 acaaaaaagg tcaggtgttt gattacgatc tggaggcgct ggccttcatc ggtaagcagc
     6721 aagaagagcc ggagcatttc cgtctggatt acttcagcgt gcagtctggc tctaacgata
     6781 tcgccaccgc cgccgtcaaa ctggcctgtg gcgaagaagt caaagcagaa gccgccaacg
     6841 gtaacggtcc ggtcgatgcc gtctatcagg caattaaccg catcactgac tataacgtcg
     6901 aactggtgaa atacagcctg accgccaaag gtcacggtaa agatgcgctg ggtcaggtgg
     6961 atattgtcgc caactacaac ggtcgccgct tccacggcgt cggcctggcc accgatattg
     7021 tcgagtcctc cgccaaagcc atggtgcacg tactgaacaa tatctggcgt gccgcagaag
     7081 tcgaaaaaga gttgcaacgc aaagctcaac acaacgaaaa caacaaggaa accgtgtgat
     7141 gtcgaagaat taccatattg ccgtattgcc gggagacggt attggtccgg aagtgatgac
     7201 ccaggcgctg aaagtgctga atgccgtgcg caaccgcttt gcgatgcgca tcaccaccag
     7261 ccattacgat gtaggcggcg cagccattga taaccacggg caaccactgc cgcctgcgac
     7321 ggttgaaggt tgtgagcaag ccgatgccgt gctgtttggc tcggtaggcg gcccgaagtg
     7381 ggaacattta ccaccagacc agcaaccaga acgcggcgcg ctgctgccgc tgcgtaagca
     7441 cttcaaatta ttcagcaacc tgcgcccggc aaaactgtat caggggctgg aagcattctg
     7501 tccgctgcgt gcagacattg ccgctaacgg cttcgacatc ctgtgcgtgc gcgaactgac
     7561 cggcggcatc tatttcggtc agccaaaagg ccgcgaaggt agcggacaat atgaaaaagc
     7621 gtttgatacc gaggtgtatc accgttttga gatcgagcgt attgcccgca tcgcgtttga
     7681 atctgctcgt aaacgtcgcc acaaagtcac ctcaatcgac aaagctaacg tgctgcaatc
     7741 ctctatttta tggcgtgaaa tcgtcaacga gatcgccacg gaatacccgg atgtcgaact
     7801 ggcgcatatg tacatcgaca acgccaccat gcagctgatt aaagatccat cacagtttga
     7861 cgttctgctg tgctccaacc tgtttggcga cattctgtct gacgagtgcg caatgatcac
     7921 tggctcgatg ggcatgttgc cttccgccag cctgaacgag caaggttttg gactgtatga
     7981 accggcgggc ggctcggcac cagatatcgc aggcaaaaac atcgccaacc cgattgcaca
     8041 aatcctgtcg ctggcactgc tgctgcgtta cagcctggat gccgatgatg cggcttccgc
     8101 cattgaacgc gccattaacc gtgcattaga agaaggcatt cgcaccgggg atttagcccg
     8161 tggcgctgcc gccgttagta ccgatgaaat gggcgatatc attgcccgct atgtggcaga
     8221 aggggtgtaa tcatggctaa aacgttatac gaaaaattat tcgacgctca cgtagtgtac
     8281 gaagccgaaa acgaaacccc gctgttatat atcgaccgcc acctggtgca tgaagtgacc
     8341 tcaccgcagg cgttcgatgg tctgcgcgcc cacggtcgcc cggtacgtca gccgggcaaa
     8401 accttcgcca ctatggatca caacgtctct acccagacca aagacattaa tgcctgtggt
     8461 gaaatggcgc gcatccagat gcaggagctg attaaaaact gcaaagaatt tggcgtcgag
     8521 ctgtatgacc tgaatcaccc gtatcagggg atcgtccacg taatggggcc ggaacagggc
     8581 gtcaccttgc cggggatgac cattgtctgc ggcgactcgc ataccgccac ccacggcgcg
     8641 tttggcgcac tggcctttgg tatcggcact tccgaagttg aacacgtact ggcaacacaa
     8701 accctgaaac agggccgtgc gaagaccatg aaaattgaag tccagggcaa agccgcgccg
     8761 ggcattaccg caaaagatat cgtgctggca attatcggta aaactggcag cgcaggcggc
     8821 accgggcatg tggtggagtt ttgcggcgaa gcaattcgtg atttaagcat ggaaggtcgt
     8881 atgaccctgt gcaatatggc gatcgaaatg ggcgcaaaat ccggtctggt tgctcctgac
     8941 gaaaccacct ttaactatgt caaaggccgt ctgcatgcgc cgaaaggcaa agatttcgac
     9001 gacgccgttg cctactggaa aaccctgcaa accgacgaag gcgcaacttt cgataccgtt
     9061 gtcactctgc aagcagaaga aatttcaccg caggtcacct ggggcaccaa tcccggccag
     9121 gtgatttccg tgaacgacaa tattcccgat ccggcttcgt ttgccgatcc ggttgaacgt
     9181 gcgtcggcag aaaaagcgct ggcctatatg gggctgaaac cgggtattcc gctgaccgaa
     9241 gtggctatcg acaaagtgtt tatcggttcc tgtaccaact cgcgcattga agatttacgc
     9301 gcggcagcgg agatcgccaa agggcgaaaa gtcgcgccag gcgtgcaggc actggtggtt
     9361 cccggctctg gcccggtaaa agcccaggcg gaagcggaag gtctggataa aatatttatt
     9421 gaagccggtt ttgaatggcg cttgcctggc tgctcaatgt gtctggcgat gaacaacgac
     9481 cgtctgaatc cgggcgaacg ttgtgcatcc accagcaacc gtaactttga aggccgccag
     9541 gggcgcggcg ggcgcacgca tctggtcagc ccggcaatgg ctgccgctgc tgctgtgacc
     9601 ggacatttcg ccgacattcg caacattaaa taaggagcac accatggcag agaaatttat
     9661 caaacacaca ggcctggtgg ttccgctgga tgccgccaat gtcgataccg atgcaatcat
     9721 cccgaaacag tttttgcaga aagtgacccg tacgggtttt ggcgcgcatc tgtttaacga
     9781 ctggcgtttt ctcgatgaaa aaggccaaca gccaaacccg gacttcgtgc tgaacttccc
     9841 gcagtatcag ggcgcttcca ttttgctggc acgagaaaac ttcggctgtg gctcctcgcg
     9901 tgagcacgcg ccctgggcat tgaccgacta cggttttaaa gtggtgattg cgccgagttt
     9961 tgctgacatc ttctacggca atagctttaa caaccagctg ctgccggtga aattaagcga
    10021 tgcagaagtg gacgaactgt ttgcgctggt gaaagccaat ccggggatcc atttcgacgt
    10081 ggatctggaa gcgcaagagg tgaaagcggg agagaaaacc tatcgcttta ccatcgatgc
    10141 cttccgccgc cactgcatga tgaatggtct ggacagcatc ggcctgaccc tgcagcacga
    10201 cgacgcgatt gcgtcttatg aagcaaaaca acctgcgttt atgaattagc ccccttgccc
    10261 ggtcaaatga ccgggctttc cgctatcgtc cacgtcatca aacgtcttta acctttgcgg
    10321 ttaaaaataa tgcgacaaca gaaataatcg caattaccca gtagacagca aagtgccccc
    10381 aactttgtgc aattgctccc tgaataacac cagccagaat taccccggta gagatagtgt
    10441 tggtaaataa ggtggtagct gcccccgctc ttccaggcat taaatcctga aaccatagca
    10501 tcccaatacc cgcaacaatg ccgataaata cagcgttaaa aagttgcagc gtcatcaacg
    10561 ccatacggct atgaaagaaa atcaatccag tgtaaaacag cactcctgcc accactgcaa
    10621 tgaccatcat tcgccgctta ccataacgtt tgacatagta gccagccaga atcattgctg
    10681 gtatttccag tccagctgcc gtccccatca ggaaacccgc cagtttgtct ggcaatccta
    10741 aatcgctact gatccacaac ggcatatcaa taatgtacat agtgttgcag gtccacatta
    10801 acgtcgaggc gacaaataac atccgtacgt tactatcctg ccagtcacct tgcattgata
    10861 aagcattttc cgacggcagt tctacccgcg ccacagacgg aagcataaat gcaatcaata
    10921 ccagactgag tgtgaatatc ccggcggcaa tcgaaaacat cactgtaaag ccgtaattca
    10981 acgccagcat aaaggccagc ggtggaccga ttacccatgc cagagaaagc tgcgcacgca
    11041 tcaccgagct aaacatcacc acttctcgcg ccgagttatc cgcgtattcc cgcgccagag
    11101 caaataactg tggcattgcc gtattggcca gagatgccag aagcacacca caggtgataa
    11161 gcgtcagata atgacgatta aatgcaaaca gtagcgcatt gccaatagcc atcaaacagc
    11221 aaaatataat cagttttcgc cgatcgccct gactgtcaga acgttttgcc aaccagaggc
    11281 ttacgccgat cccagcaata gcattcaccg tataaaagag tccgatccag aaaggttgcg
    11341 cgccaacctc acgactcaga aataagctca atgtaggagc ctgtagcgcc ccggccaccc
    11401 ccatcataaa agcgaccagc ataaatgccg cgtaaacacc gttcatacgg cgagccatcg
    11461 tcattatcca gatcatacgt tcccttttta gcgcggcgag aataaaaaaa accagcaggt
    11521 ataatctgct ggcgggtgat tttacaccaa tactcagtca cacatgatgc aggcaagtca
    11581 actttcagaa ttgcggtcat cccactgcat cagtccttca agcatggtta atcgttgtgg
    11641 gacgcttaac caacgcaacc agcacaactt cgctgtcgcg gtaaaatagt gctgataaaa
    11701 ctgacgcatg gggcatctcc ttgcttcatc gttaggaatt attgacttaa tatagggaaa
    11761 ataaaattgc tgtcttttgc gcaggagttc cccttttatg ccatctgctc gtctgcaaca
    11821 acagttcatc cgcctgtggc aatgctgcga gggtaaatcg caggacacaa cgctcaacga
    11881 actggcagcg ttattgagct gctcgcgtcg tcatatgcgt accctgctca acaccatgca
    11941 ggatcgcggc tggctgacgt gggaagcgga agtcgggcgc ggtaaacgct cgcgtctgac
    12001 attcctctat accgggctgg cgcttcagca acagcgggcg gaagacctgc tggagcagga
    12061 tcgtatcgac caactagtgc agttggttgg cgacaaagcg actgtgcggc aaatgctggt
    12121 ttctcatctg ggccgcagct tccgccaggg gcggcacatc ctgcgcgtgc tctactatcg
    12181 tccgttgcgt aatctgctac ctggcagcgc attgcgccgt tccgaaaccc atattgcccg
    12241 gcaaatcttc agttcgctaa cgcgcataaa tgaggaaaat ggggaactgg aagcagacat
    12301 cgcccaccac tggcagcaaa tatcaccgct tcactggcgt ttctttttgc gtccaggagt
    12361 ccattttcat catggtcgtg aactggaaat ggacgacgtg atcgcctctt taaaacgaat
    12421 caatacgctg ccgctctatt cgcatattac ggacattgtg tcgccgacgc cctggacgct
    12481 ggatatccat ctcacgcagc cggaccgctg gttgccgtta ctgctggggc aagttccggc
    12541 gatgatcctg ccgtgcgaat gggaaaccct cagtaacttt gccagccatc ctattggcac
    12601 cggtccgtat gcggtgattc gcaacagcac caatcaactg aaaattcagg cattcgatga
    12661 cttcttcggt taccgggcat taatcgacga agttaacgtc tgggttctgc cggaaattgc
    12721 cgacgagcca gccggtgatc tgatgctaaa aggtccacag ggcgaggaaa aagagattga
    12781 aagccgcctg gaggaaggtt gctactattt actgttcgac agccgcaccc atcgcggggc
    12841 gaatcagcaa gtcagggact gggtaagcta tgtgctttct ccaactaatc tggtctattt
    12901 cgctgaggaa cagtaccagc aactgtggtt cccggcttat ggactgctcc cccgttggca
    12961 ccatgcccgc accataaaga gcgaaaaacc ggctggcctg gaaagcctca ccctgacctt
    13021 ttatcaggat cacagtgagc atcgggtgat tgccgggatc atgcagcaga ttctggcaag
    13081 tcaccaggtc acgctggaaa tcaaagagat cagctacgat cagtggcatg aaggagagat
    13141 cgagagcgat atctggctta atagcgccaa ctttacgctg ccgctggatt tttcgctgtt
    13201 cgcgcacttg tgcgaggtgc cgctgctcca acactgtatt ccgatcgact ggcaagccga
    13261 cgccgcccgc tggcgcaatg gcgaaatgaa cctggcgaac tggtgccagc aactggtcgc
    13321 cagtaaagca atggtgccgc ttatccacca ctggctgatc attcagggac aacgcagtat
    13381 gcgcggcctg cgtatgaata ccctcggctg gttcgatttt aaatcagcgt ggtttgcgcc
    13441 accggatcca tgattgctgg tagataacaa aatcactaca ctaacgccgt tctcaacggg
    13501 gtgccacgca aacgcgtgcg ctgagaaaat acccgtcgaa cctgatccgg ataacgccgg
    13561 cgaagggatt tgaggctcct tctcaagtcc tttgccactc ttttttgagg tgcaaagtgt
    13621 taaaaaaatg tcttcccctg ctgttgctgt gcacagcgcc cgttttcgct aaacccgttc
    13681 tgactgttta tacctacgat tccttcgccg ccgactgggg gcctggtcca aagatcaaaa
    13741 aagcttttga agccgactgt aactgcgaac tgaaactggt ggcgctggaa gatggcgttt
    13801 cgcttctcaa ccgtctacgg atggaaggca aaaatagtaa agccgatgtg gtgctggggc
    13861 tggataacaa tctgttagac gccgccagta aaaccggact atttgccaaa agcggtgtgg
    13921 cagcggatgc cgttaacgtt cccggcggct ggaataatga cactttcgtc ccgtttgatt
    13981 acggctattt cgccttcgtc tatgacaaga acaaactgaa aaacccgccg caaagcctga
    14041 aagaattggt tgagagcgat cagaactggc gggtgattta tcaggatccg cgtaccagca
    14101 cgccggggct gggtctgctg ttgtggatgc aaaaagtcta tggcgatgac gccccacaag
    14161 cctggcaaaa actggcgaag aaaaccgtca cggtcaccaa aggctggagc gaagcctacg
    14221 gcctgttttt aaaaggtgaa agcgatctgg tactgagtta caccacctct ccggcttatc
    14281 acattctcga agagaaaaaa gataactacg ccgccgcgaa ctttagcgaa ggtcactatc
    14341 tgcaggtgga agtcgccgcc cgcaccgctg ccagcaagca gccggagctg gcgcaaaaat
    14401 tcctccagtt tatggtttct ccggcttttc agaatgcgat cccaaccggt aactggatgt
    14461 atccggtggc aaatgtcacg ctgcctgccg ggtttgaaca attgaccaaa ccggccacca
    14521 cgctggagtt cacaccagct gaagtggcgg cacaacgtca ggcatggatt agcgaatggc
    14581 aacgcgccgt cagccgttaa ttcccggctg gttaattcca ggtgtaagcg ccgccacgct
    14641 ggtggtagcg gttgcgctcg cggcgtttct cgccctgtgg tggaacgcgc cgcagggtaa
    14701 ctgggtggca gtctggcagg acagctacct gtggcatgtg gtgcgcttct ccttctggca
    14761 ggcgtttctc tcggcgctac tctctgtcgt acccgcgata ttcctcgccc gcgccctcta
    14821 tcgcaggcgc tttccgggtc ggctggcgct gttgcgtctg tgcgcaatga ccttgatcct
    14881 cccggtgcta gttgctgttt tcggcattct tagcgtctat ggtcgtcagg gctggctggc
    14941 atcgctctgc caatcgctcg gtctggagtg gaccttttcg ccctacggcc tgcaaggcat
    15001 tttgctggcg cacgtatttt ttaatctgcc gatggcgagc cgcttattac tccaggcact
    15061 ggaaaacatt cccggcgaac aacgtcagct tgccgcccag cttgggatgc gcggctggca
    15121 ttttttccgc ttcgtcgaat ggccgtggct acggcgacaa atcccgccgg ttgccgcgct
    15181 tatctttatg ctctgtttcg ccagcttcgc caccgtgcta tcgctgggcg gcggtccgca
    15241 ggcaaccact atcgagctgg ctatttatca ggcgctgagt tacgactacg atcctgcccg
    15301 cgcggcgatg ctggcgctgc tccagatggt gtgttgcctt gggctggtgc tgttgagtca
    15361 gcgactaagt aaggccattg cgcccggcac cacgctgctg caaggctggc gcgatccgga
    15421 cgatcgtctg catagccgca tttgcgacac ggtgttaata gtgctggcgc tgctgctgtt
    15481 gctgccaccg ttgctggcgg tgatcgtcga tggggtaaat cgccagttgc cggaagtgct
    15541 ggcacaaccg gtgctgtggc aggcgctgtg gacctcgttg cgtattgcgc tggcggcagg
    15601 tgtattgtgc gtagtgctga ccatgatgct gctatggagc agtcgcgaac tgcgggcgcg
    15661 gcagaaaatg ctggcgggtc aggcgctgga gatgagcggc atgttgatcc tcgccatgcc
    15721 ggggattgtg ctggctaccg gcttcttttt actgctcaac aacactatcg gcctgccaca
    15781 atctgctgac ggcattgtga ttttcaccaa tgcgttaatg gcgatccctt atgcgctgaa
    15841 agtactggaa aacccgatgc gcgatatcac cgcccgctac agtatgttgt gtcagtcgct
    15901 gggcattgaa ggctggtcgc gcttaaaagt ggtcgagctg cgcgccctga aacgtccact
    15961 ggcgcaggcg ctggcttttg cctgcgtgct gtcgattggt gattttggcg tagtggcgtt
    16021 gttcggtaac gatgatttcc gtaccctgcc gttttatctc taccagcaaa ttggctccta
    16081 tcgcagccag gacggcgcgg tcaccgcgtt aattctgcta ctactctgtt ttctgctgtt
    16141 taccgtgatt gaaaaactac cggggcgaaa tgttaaaact gactgatatc acctggcttt
    16201 atcaccattt gccgatgcgt tttagcttaa cggtagaacg cggcgagcag gtggcaatcc
    16261 tcgggccaag cggcgcgggt aaaagtaccc tgctgaattt gattgccggt tttctgacgc
    16321 cagccagcgg ttcgctgact atcgatggcg ttgatcacac cacgacgccg ccgtcacgcc
    16381 gtccggtgtc gatgctgttt caggagaaca acctgttcag ccacctgacg gtcgcgcaga
    16441 acatcgggct ggggctaaat ccggggttga aactgaacgc ggcacagcag gagaaaatgc
    16501 acgcgattgc ccgtcagatg gggattgata atttaatggc gcggttaccg ggcgagcttt
    16561 ccggcggtca gcgacagcgc gtggcgttag cgcgttgtct ggtgcgcgaa cagccgattt
    16621 tattgctcga tgaaccgttc tctgcgctcg atccggcgtt acgtcaggag atgctgacgc
    16681 tggtgagcac gagctgccag cagcaaaaaa tgacgctatt gatggtgtcg cacagtgtgg
    16741 aagatgcggc gcggatcgcc acacgctcgg tggtggtcgc cgacgggcgc atcgtctggc
    16801 agggtaagac tgaagaattg ttgagcggta aggcgagtgc ttcggcaata ttggggatta
    16861 cgggttagta tgcactgtca gatgcggcgt gaacacctta tccgacctac ggacagtgcg
    16921 ttttgtaggc ctgataagac gcggtcagcg tcgcatgagg caacccgcac aagacaatcc
    16981 cctaaacccc aaccacttta cgcaaaatat cgatatacac cggcatcaac gggtggcgaa
    17041 ttaacaccac cagcgccacc acgccgatgg cagaaatcaa cggcgtcagc cacaacaaac
    17101 ggccgcgcga caaataatga cttaaacgat cggttgcttt accgctgcgc cataaccgcc
    17161 agcacagcca gccaccaacc cacaaaaata ccgccgtcgc cagcagcaac catttaaact
    17221 cgccgctctg cattccggct ggaatatcga tcgccgcgcc cgccagaatc ccaggcagga
    17281 agtaaaacgg tggccacagc aggcagccga taatattcgg cgtaatgaat tttgccaccg
    17341 gcagatccag cattcccgcc accattggca ccagcggacg cgtcgggcca acaaagcgac
    17401 cgaccagaat ggtgaacatg ctgtgttgat gcaacgcatg ttcagtttta tcgagtaatg
    17461 ctttgttttt cttcagaaat gaccagcgat gcaacggctt tttaaaacgc cagcccagcc
    17521 agaaggagat ccagtcgccc agcagacaac cgacaatccc tgccagccaa gcgtgccaga
    17581 aacttaactc gccgctgcca atcagcgctc ccagccccgc catcagcacc gtaccgggta
    17641 gaatcaaacc gaccagcgcc agcgactcca gaaaggccac caacaccacc gccatcaatg
    17701 aatacacggt agattgggta ataaagtgtt ccagcaatgc ttgcataatg tgcctgtcaa
    17761 atggacgaag cagggattct gcaaacccta tgctactccg tcaagccgtc aattgtctga
    17821 ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc acaaccggca
    17881 cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga
    17941 tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc
    18001 agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc
    18061 tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg
    18121 atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacacga
    18181 ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc
    18241 tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg
    18301 atttgcccaa acaggtcgct gaaatacggc tggtgcgctt catccgggcg aaagaaaccc
    18361 gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag
    18421 taaacccact ggtgatacca ttcgcgagcc tccggatgac aaccgtagtg atgaatctct
    18481 cctggcggga acagcaaaat atcacccggt cggcagacaa attctcgtcc ctgatttttc
    18541 accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg
    18601 tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg
    18661 gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata
    18721 ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc
    18781 gtcttttact ggctcttctc gctaacccaa ccggtaaccc cgcttattaa aagcattctg
    18841 taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc
    18901 agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta
    18961 tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca
    19021 tacccgtttt tttggatgga gtgaaacgat ggcgattgca attggcctcg attttggcag
    19081 tgattctgtg cgagctttgg cggtggactg cgccagcggt gaagagatcg ccaccagcgt
    19141 agagtggtat ccccgttggc aaaaagggca attttgtgat gccccgaata accagttccg
    19201 tcatcatccg cgtgactaca ttgagtcaat ggaagcggca ctgaaaaccg tgcttgcaga
    19261 gcttagcgtc gaacagcgcg cagctgtggt cgggattggc gttgacagta ccggctcgac
    19321 gcccgcaccg attgatgccg acggtaacgt gctggcgctg cgcccggagt ttgccgaaaa
    19381 cccgaacgcg atgttcgtat tgtggaaaga ccacactgcg gttgaagaag cggaagagat
    19441 tacccgtttg tgccacgcgc cgggcaacgt tgactactcc cgctatattg gcggtattta
    19501 ttccagcgaa ggttctgggc aaaaatcctg catgtgactc gccaggacag cgccgtggcg
    19561 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    19621 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc tctgtggcac
    19681 gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    19741 cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    19801 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    19861 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    19921 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    19981 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    20041 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    20101 ccgctggaac agcttgccgc ccagcatgcg gaactgaaag cgcaaatcaa cgccagccag
    20161 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    20221 ccggtggtgc tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
    20281 ggggtgatta ccgatcttaa cctcgctacc gacactccgc tgctgttcgg cggtttgatt
    20341 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    20401 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    20461 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    20521 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    20581 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    20641 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    20701 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    20761 gacgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    20821 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    20881 agcgaaactg ccctgcaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    20941 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    21001 caccttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    21061 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    21121 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    21181 tcagcaacat gccgtcgtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    21241 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgatt
    21301 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacagatcaa
    21361 gttcggtttc tccgtcaata cctgggcggt tggcgatctg gtgcaggtgg tgaactccat
    21421 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    21481 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    21541 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    21601 tttgcacggt ctgaaacagc ttccaggtct gcccgtacag cgtctgatgc agcagggtta
    21661 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    21721 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    21781 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    21841 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    21901 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    21961 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    22021 actgccggtg gcgaatgcac tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    22081 gtggatcctc gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    22141 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    22201 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    22261 agtagccaca tcaggtatgt aacgcctgat gcgacgctga ctcgtcttat ctggcctaca
    22321 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac
    22381 gctggcgcgt cttatcaggc ctacgcgctg cgattttgta gaccggataa gcgaagcgca
    22441 tccggcacga aggagtcaac atgttagaag atctcaaacg ccaggtatta gaagccaacc
    22501 tggcgctgcc aaaacacaac ctggtcacgc tcacatgggg caacgtcagc gacgttgatc
    22561 gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga ttacagcgtc atgaccgctg
    22621 acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg aaaaagccct
    22681 cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
    22741 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa
    22801 ccggcaccac ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg
    22861 acgcagaaat caacggcgaa tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg
    22921 aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct ggtccattcc cacggcccgt
    22981 ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg
    23041 cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc
    23101 tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac
    23161 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg
    23221 gattttcagc gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca
    23281 agggacagaa gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc
    23341 accgcaagag tccgtggcgt ttattcacgc cgatcaggtt ccccgcgctc agcatatttt
    23401 gcagggtgaa caaggctttc gtctgacacc gctggcgtta aaggattttc accgccagcc
    23461 ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgcctgcg
    23521 tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga
    23581 gcggtttatc acctcaccgg tgtgggtcga aggtgatatg cacaatggca ctatcgttaa
    23641 tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat
    23701 tgaaaccacc cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat
    23761 cgtttatatg ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata
    23821 cgtcgccagc cgcccgctgc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc
    23881 cgatgtgatc atcggctgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc
    23941 cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
    24001 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg
    24061 tatcgaggcg ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc
    24121 tcaggagcta ttaggcgaag gaaaatctat cgataacccg tgggttcgaa tggacgaaat
    24181 tgaccgccgt ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga
    24241 gctggtgacg cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac
    24301 ggtgaacggc ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta
    24361 ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
    24421 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt
    24481 gctggactat aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg
    24541 gctggttgaa ggcatggcgc aacctgatcc agagcacagt accgaaggtt ttctcgatgc
    24601 ctggttctca cgggagaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg
    24661 cgatgaagcc aaacgtcagg gtaataagcc gctttcgcag gcgctgaaga tcattatgaa
    24721 tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc
    24781 gtcgatcacc atgcgtggtc atcagatcat gcggcagacc aaagcattga ttgaagcgca
    24841 gggctacgac gttatctacg gagataccga ctcgactttt gtctggctaa aaggtgcaca
    24901 ttcggaagaa gaggcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg
    24961 ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaactca
    25021 tttctgccgt tttcttatgc caaccattcg cggagctgat accggcagta aaaagcgcta
    25081 cgccgggctg attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt
    25141 gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctgtacc tgcgtatctt
    25201 ccgcaacgag ccatatcagg agtacgtccg ccaaaccatc gacaaactga tggcgggtga
    25261 actggatgcg cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg
    25321 taatgttccg cctcatgtac gcgccgctcg ccttgccgat gaagaaaatc aaaagcgcgg
    25381 tcgccccttg caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc
    25441 ggagccgctg gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct
    25501 acaacccgtg gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac
    25561 cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat tttgatgcgt
    25621 gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
    25681 cactacctca taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga
    25741 acacatatgc cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga
    25801 cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt
    25861 gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt
    25921 gataccatta ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat
    25981 ggcttgctga cctatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt
    26041 gaagttttcc ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg
    26101 cagattgacc gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa
    26161 cagttccgta tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcatcag
    26221 ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc gcgtcctgct ggctgacgaa
    26281 gtgggtttag ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc
    26341 gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa
    26401 atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct
    26461 cagcacgatg cttacaaccc gtttgacacc gaacagctgg tgatttgctc gctggatttt
    26521 gcccgtcgta gcaaacagcg cctggaacat ctctgtgaag ccgaatggga cctgctggtg
    26581 gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc caagccgtga atatcaggcc
    26641 attgaacaac tggcagagca cgtgccgggc gttctgctgc tgaccgcgac cccggaacag
    26701 ctggggatgg aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat
    26761 tttgcgcagt tcgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg
    26821 ctgctggcag gtaacaaact gagcaatgac gaactgaaca tgctcggcga gatgatcggc
    26881 gagcaggata tcgagccgct gttgcaggca gcaaacagcg acagcgaaga tgcccagagc
    26941 gcccgtcagg agctggtttc gatgctgatg gatcgccacg gcaccagccg cgtgctgttc
    27001 cgtaacacgc gtaacggtgt gaaaggattc ccgaaacgcg agctgcacac cattaagctg
    27061 ccgctaccga cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgtaaa
    27121 agtgcggaag atcgtgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa
    27181 ggtgataacg ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg
    27241 accagccatc gctctcagaa agtgctggtg atctgcgcca aagctgccac tgcgctgcaa
    27301 ctggagcagg tactgcgcga acgtgaaggt attcgcgctg cggtgttcca cgaaggtatg
    27361 tcgattatcg aacgtgaccg cgctgccgcc tggtttgccg aagaagacac cggcgcacag
    27421 gtactgctgt gctcagaaat cggttctgaa ggacgtaact ttcagttcgc cagccacatg
    27481 gtgatgtttg acctgccatt caacccggat ctactggagc agcgtattgg tcgtctggat
    27541 cgtatcggcc aggcgcacga tattcagatc catgtgcctt atctggagaa aaccgctcag
    27601 tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat ttgagcacac ctgcccgacc
    27661 ggacgcacta tttacgatag cgtatacaac gatctgatta actatctggc ttcaccggat
    27721 caaaccgaag gctttgacga tctgatcaaa agctgccgcg agcaacatga agcgctgaaa
    27781 gcacagctgg aacagggtcg tgaccgcctg ctggaaatcc actccaacgg tggcgaaaaa
    27841 gcccaggcac tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc
    27901 gccatgaacc tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc
    27961 gtgctgacgc cgtccgatca tatgctggtg ccggacttcc ctggcctgtc ggaagatggc
    28021 atcaccatca cctttgatcg tgaagtggcg ctggcgcgtg aagatgcaca gtttattacc
    28081 tgggagcatc cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc
    28141 agcacgattt cactgttaaa aaacaaagcg ttgccggtag gtacgctgtt ggtggaactg
    28201 atttatgtgg ttgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccaccg
    28261 acgccggtac gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtagagttt
    28321 gaaaccttta accgccagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac
    28381 accgtgcagc aggatgttca cgctatcctt caactgggtg aagcgcagat cgagaaatct
    28441 gcccgtgcat tgattgatgc agcgcgtaac gaagccgacg aaaaactgtc tgccgagctg
    28501 tctcgtctgg aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc
    28561 attgagagca accgtcagca ggtaatggaa agcctggatc aggcaggttg gcgtctggat
    28621 gccctgcgtt tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact
    28681 acaatccacc gcaggaaccc tggttggtta tcctgtatca ggatgaccat attatggtgg
    28741 tcaacaagcc gagcggtttg ttgtcagtgc cgggtcgtct ggaagagcac aaagacagcg
    28801 tgatgacgcg cattcagcgt gattatccgc aggcagaatc ggtgcatcgt ctggatatgg
    28861 ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc
    28921 agttccgcga gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc
    28981 ccgcagaagg tctggtggat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga
    29041 aagtctgtta cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg
    29101 cggataacac ggcaagagcg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg
    29161 tgcatatgct ggcgctgggg catccgattc tcggcgatcg tttttatgca tcaccagaag
    29221 cgagagcgat ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg
    29281 cgtatggcaa tagtatgacg tttaaagcgc cagcggattt ttaatcgtca cgctggcgca
    29341 gttcggtagg cggataaggc gttcacgccg catccggcaa ttgtgcgccg attgcctgat
    29401 gcgacgcggg ccgcgtctta tcaggcctac agtttaaaac ttatttaaac cctttctgct
    29461 gctttatcag ctcatatgcc tgctgaattt cctgcgcttt ctgcttcgcc atctccatca
    29521 tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc atcagcttac
    29581 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc acattacagg
    29641 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
    29701 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
    29761 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
    29821 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
    29881 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattatctg
    29941 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
    30001 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
    30061 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
    30121 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
    30181 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
    30241 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
    30301 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
    30361 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
    30421 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
    30481 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
    30541 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
    30601 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
    30661 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
    30721 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
    30781 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
    30841 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
    30901 aaagatacct acgtctggga aggtgattac cagatggtgg gtcgccaggg tcgcggtaaa
    30961 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
    31021 acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat tattcatgac
    31081 cgcgaagaac aagttgcgga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
    31141 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
    31201 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
    31261 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
    31321 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
    31381 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
    31441 tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
    31501 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
    31561 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
    31621 accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca
    31681 gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga tacgcgtatt
    31741 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aacccgtgtt
    31801 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
    31861 gcgaagtttc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
    31921 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
    31981 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
    32041 cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg
    32101 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
    32161 cgtattgcct ccgctaacca ggtgacgacc ggtgtcacat ctcgcatata tgatgatgct
    32221 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
    32281 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
    32341 gacacctact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca
    32401 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
    32461 ctggtacagc tgaattacca ttacgccagc ccggaatata ttcaggctac gctgcctaag
    32521 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
    32581 aggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
    32641 aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
    32701 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
    32761 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
    32821 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
    32881 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
    32941 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
    33001 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
    33061 ggtaaaactg aacgctgcgc aggcaaggca gcagcttcct gatgacgcga cgctgcgcca
    33121 ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg
    33181 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
    33241 catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact acaacactta
    33301 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
    33361 tcgcatcacc atcctgccgc aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
    33421 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
    33481 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
    33541 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
    33601 cggccagatg gggtggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
    33661 caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct
    33721 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
    33781 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
    33841 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
    33901 ctcgcaggat ccaggttctg ctaaccaggg cggtgatctc ggctgggcta caccagatat
    33961 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
    34021 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
    34081 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agctctcgga
    34141 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
    34201 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
    34261 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
    34321 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgcccttat
    34381 tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact tcctgtcgcg
    34441 ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta cgtggtggag
    34501 acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct gatcacaggc
    34561 cccgtgcata aaggcgtcat taacgacgca agcattccgt ttaccggtca taccgagttt
    34621 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
    34681 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgca
    34741 cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt tggtattacc
    34801 gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg tcatatgggt
    34861 acggaagaga tagacaccat tattccggtg ctcgacgagc tgcgggcgca ggggatgaaa
    34921 ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
    34981 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
    35041 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
    35101 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
    35161 ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
    35221 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
    35281 ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt cttgcggcat
    35341 tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
    35401 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
    35461 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
    35521 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
    35581 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
    35641 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaacgtga
    35701 tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
    35761 tcgtgcgcct ggtccctcat gcaacgatgc ctcacccggt taaagatgtt cgcgtgttga
    35821 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
    35881 acctgtttag cgtcgaggtg ttaaccggaa tggggatcga cccggcgatg cgagcggaaa
    35941 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
    36001 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
    36061 tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
    36121 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ccaatggcaa
    36181 tggccgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
    36241 tggcgaagag taccagtaca ccagcggtgc aatcattgaa accccgctgg gcaccatgca
    36301 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
    36361 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
    36421 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccccgg
    36481 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt cgctggatgt
    36541 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
    36601 gcatctgctg gcggtatttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
    36661 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
    36721 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
    36781 tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
    36841 tcccttcttt cttgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
    36901 ggggctggga agactgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgcttccc
    36961 gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
    37021 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
    37081 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata ccggctgctg
    37141 ctggggtggg tcattaacct gcctgcgctg ggaagataaa cagtattttg tccagccgtc
    37201 gaaccggcat aaggatttgg gcgaagcggc ggcgtcttaa acacagcctg atacaggaag
    37261 gccggataag acgcgaccgg cgtcgcatcc ggcactagcc gtaaattcta tacaaactta
    37321 ccgccgctcc agaatctcaa agcaatagct gtgagagttc tgcgcatcgg cgtcgtggaa
    37381 ttcgctgaat accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc
    37441 cacttctgcg tcgatatgcg tcagatacag tttttgcgct tttggcagga actgctcata
    37501 aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg caatggcttc
    37561 atccaccgat ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat
    37621 atttttgcgt cctggcaacg gacgaccgat tgattcccaa gtatggcggc ccataatcac
    37681 gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat
    37741 ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga
    37801 gatttcccga taaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    37861 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg
    37921 actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca
    37981 ctctttatta agatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac
    38041 cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg
    38101 tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt
    38161 cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    38221 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccggttt
    38281 tcagcgcacc ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    38341 tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca
    38401 ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg
    38461 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt
    38521 tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt
    38581 tcaccccgct ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcga
    38641 taatcacgcg cggctgttct tcatcgatct catcggcttc acgcgcctcc tccgtagaag
    38701 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca
    38761 ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcac
    38821 caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac cagcgacgct
    38881 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    38941 ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg ccaaagtcta
    39001 tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaac ggttcgatat
    39061 cgctttccaa cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    39121 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata
    39181 acgccacggc actaaacact tcccgcaagc cagagcgggc aacaaaacgc agcgccggac
    39241 gcgtaacata gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg
    39301 agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc gggatcatcg
    39361 ccaccagcgg gattgccgcg atatcctgga acagcagcac tgcaaaggca ctgcggccca
    39421 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gttgatgaga
    39481 gcgccagcgt cataccgatc aattccgcga cctgccagcg cagcccaagt aacatacaga
    39541 acagccccag caggccaccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    39601 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    39661 ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc
    39721 acgggccaat aatgcagccg gcgatcaggt agccaagtac cgatcccaga ccaagacgta
    39781 ccgcaatggg tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat
    39841 gactatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc
    39901 ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag
    39961 ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga
    40021 tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt
    40081 ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg
    40141 atccaaagtt taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc
    40201 agatcggcgc gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag
    40261 agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt
    40321 ttattcgcat gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt
    40381 agcccgcgtg tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    40441 tagtcaataa tattgattca gaaaagagag aaatctgaac aaataatccc attctctgcc
    40501 cgcggatcgt catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg
    40561 ctacggaaat caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga
    40621 taccgtaatt attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac
    40681 ttaaggacat aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag
    40741 gatagagcca ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca
    40801 tcactaccag ccagatcccc atatccggga ttagccccaa aaccgccagc gccagcgtca
    40861 tcatggcaaa gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg
    40921 gcggaataca gccgagcata aagaacaggc taatcaccac attccctagt gccgcgtttt
    40981 tgccaacccc caatcccaac gaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca
    41041 ttgggatcac ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc
    41101 gattaaacag atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt
    41161 cgccaaacag ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc
    41221 agcggggaga ttctggcagt tcgaatcgac caatcaaaat caacaaacaa gggatcgccg
    41281 cgctacccag catccagcgc caaccacctt ccacatcata aagccagtag ccgaccagat
    41341 cggcgcaggt cgcgccgaca taccacatcg cggcaataaa gctgatggaa aacgcccgct
    41401 gacgggtact ggagaactcg gtgatcatcg aagtggcgat gggataatct gcaccgatga
    41461 caatgccgat gagtacccgc atcaccaaca gttcgacggg ggatgaaaca cacatcgtcg
    41521 ccaccgatat cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt
    41581 tatcggagat atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc
    41641 ccagcaagcc aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca
    41701 cgccaatcat taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa
    41761 tgcggcggtg aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt
    41821 attcctgttt ttttgcaatg agagtcagta cggcgttacg tatcaaacca acatcagccg
    41881 taacggaact ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc
    41941 agccccagaa tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg
    42001 ccgtcatcct gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagcacc
    42061 tgtttatcag catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta
    42121 ttgacgccca gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca
    42181 tgccatcctt gatcagattg atgaagccca ctttcttgcc gtggcggagg attttcttgc
    42241 gcatcagttc cggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca
    42301 gttccgggta gccgctaaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca
    42361 tgcgcatatc gcgcagcggg ctgctctcca gatgctgacg atattccgcg agtttttgct
    42421 tactgaaatc gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg
    42481 caatcgccag atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat
    42541 caccggcaat caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg
    42601 gcactacgtg agcggaatat tccaccagct tgccgcccgc gatcagcggt gcaacggccg
    42661 gatgctgttt gaaatcttcc agcatttgcg gcaccgattt tttcgcgtca tgcagatgat
    42721 gcagaccaca aaccagcccc agcgacaggg tattttcatt ggtataaagg aagccgccgc
    42781 ccatcaggcc atcggtgggt gatcccgcaa acagacaagc cgcgccctga ttaccctgca
    42841 actgaaaacg gtcttcgatt accgacttcg gtgactcgat cagttccttc acgccaaccg
    42901 ccacatccgt cggtttaacg cgttttgcca tccccaactt ttcggcaagg atggagttca
    42961 ccccatcagc aaggagcacc gttttcgctt caatcacatc gccatcggct tctacaccga
    43021 cgactttgcc atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcgc
    43081 ccgcttcttc ggcctgctcc atcaaccagg catcaaattt actgcgcaaa acggagtaag
    43141 aacgctggga tagcgaggtt tcgtcaccgt tgcagtagtc catagtcatc gctgactttt
    43201 ccgtcataaa cgcgagtttt tcatgggtga tcaggcgttc tacggggcgg aatcggcgaa
    43261 acctggaata atgtgttcca gactgtgggc atagagacgc ccgccagtaa cgttcttggc
    43321 acccgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    43381 tgcggcaacc gaaccggcaa gccctgcacc gacgatgatg gcgtcaaaga tatcttcgga
    43441 cataagagct ccctgtcagc ggcagagccg taatgcagcc ctgccagagt ggatcaacgc
    43501 gctaaagctg cggtcagtgc cggaaggatc ttcacggcat cgccaacaat gccgtaatcc
    43561 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgacgcg
    43621 ttagcgccaa ccatgtgctg gatctgcccg gagatcctca ccgccaggta cagttcaggt
    43681 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttct
    43741 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagt
    43801 gcaatgttct ctttgctgcc aatgccgcga ccgacgctga ccaccagacg ggctttgtcg
    43861 agatcgacgc tgttgctctg gcgcgtctgg gttgccgtgc gggtaatcgc cacagccgga
    43921 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    43981 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    44041 gccagaccgc cgtaaaccat gtgtttcact gttgctttac cgtcctgtac gctgacggtg
    44101 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    44161 ccgcgacggg tgtttggcaa cagcaccaga ccgtcagcgc cgtgctggcg aatagtgtca
    44221 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    44281 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggca
    44341 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agagggggta
    44401 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    44461 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    44521 gctgacgctc gcgctgtttc ggcgcggcaa cctgttgttc agaccaggcg tccacggcgt
    44581 taaaaccaat atccgccgcc gaccatacct ggactggctt tttcgccgcg ccgagaatgg
    44641 ctttcatcga aggaatttgt ggggagttga tatcagtgga aacagcaaca accgcaggca
    44701 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    44761 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    44821 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcagat
    44881 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    44941 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agcacatctt
    45001 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggctgtc acctgcgcct
    45061 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttgagatcg tattggctta
    45121 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    45181 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    45241 aggaaacgtc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    45301 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    45361 gttaagttcc tcaccaatat tgaaaacatg gcgtagcaaa aagaaatttt caatattgtt
    45421 ttatagatca ccaatattga aagtcagatg ctatttttta cccacaaaag aacataacaa
    45481 actgattaat aaccaaaatt aacgaaaaac atcctctatt cactcgaaac aaaaatgtga
    45541 caccaatcac agaatacagc ttattgaata cccattatga gttagccatt aacgcgtcca
    45601 cgaggttaat aataattata ttaaatgtta acaaaaataa aacaaacggg aacgcaaaat
    45661 aaatattcgt tttcacagtg gaattaactc atgaagaatg aaaagagaaa aacgggaata
    45721 gaaccgaagg ttttctttcc gccattaata atcgtcggca tactttgttg gcttacagtc
    45781 agagatctgg atgcggcgaa tgtcgttatt aatgctgtat tcagttacgt caccaatgta
    45841 tggggatggg catttgaatg gtatatggtg gtgatgcttt tcggttggtt ctggctggtg
    45901 tttggcccgt atgccaaaaa gcgtttaggt aacgaaccgc cagaatttag caccgccagt
    45961 tggatcttta tgatgttcgc ctcctgtacg tctgctgccg tactgttctg gggatcgatt
    46021 gagatctact actacatctc caccccgccg tttggcttag aaccgaactc gacaggagcg
    46081 aaagagttgg ggctggctta cagcttgttc cactggggac ctctgccgtg ggccacttac
    46141 agcttccttt cagtcgcctt cgcttacttc ttctttgtcc gcaaaatgga agtgattcgc
    46201 cccagctcga cactggtgcc gctggtaggt gaaaaacacg ccaaagggtt gttcggtacc
    46261 atcgtcgaca acttctatct cgtcgccttg atcttcgcga tgggtaccag tctgggcctt
    46321 gccacgccgc tggtgaccga gtgtatgcaa tggttgtttg gcattccgca taccctgcaa
    46381 ctggacgcta tcatcattac ctgctggatt atcctcaacg ccatttgcgt cgcttgcggt
    46441 ctgcaaaaag gggtacgtat cgccagtgac gtgcgtagtt acctgagctt cctgatgctg
    46501 ggttgggtgt tcatcgtcag cggtgccagc ttcatcatga actacttcac cgattcggtg
    46561 gggatgttgc tgatgtatct gccgcgcatg ttgttctata ccgatcccat cgctaaaggc
    46621 ggcttcccgc agggctggac cgtgttctac tgggcatggt gggtgattta cgccatccag
    46681 atgagtatct tcctcgcccg catctcccgt ggtcgtactg tgcgtgaact gtgcttcggc
    46741 atggtgatgg ggctgacagc atccacctgg atcctgtgga ccgtgctcgg tagtaacact
    46801 ctgctgttga tggataaaaa catcatcaac attccaaatc tgatcgaaca gtacggtgtg
    46861 gcgcgcgcca tcatcgaaac ctgggccgct ctgccactca gcaccgccac catgtggggc
    46921 ttcttcatcc tctgctttat tgccaccgtt acgctggtta acgcctgctc ttataccctg
    46981 gcgatgtcta cttgccgcga agtacgcgat ggtgaagaac caccgctgct ggtgcgtatc
    47041 ggttggtcaa ttctggttgg cattatcggt attgttctgc tggcgctcgg cggcctgaaa
    47101 ccgattcaaa ccgccattat cgccggagga tgcccgctgt tcttcgtcaa cattatggtg
    47161 acgctctcct ttattaaaga cgcgaaacag aactggaaag attaattaac ccccaaaata
    47221 tcaagaggtt gaaagatgga ttttaattta aatgatgagc aggaactgtt tgtcgccggt
    47281 atccgcgaac tgatggccag cgaaaactgg gaggcctatt ttgccgagtg cgaccgtgac
    47341 agcgtctacc cggaacgttt tgtcaaagca ctggcggata tgggtatcga cagtctgctg
    47401 atccctgaag agcacggtgg tctggacgcg gggtttgtta ctctcgccgc cgtgtggatg
    47461 gagctgggac gtctgggggc accaacctat gtactgtacc agctgccggg cgggttcaat
    47521 accttcctgc gcgaaggcac acaagagcag atcgacaaga ttatggcttt ccgcggcacc
    47581 ggtaagcaga tgtggaactc agcgattacc gaaccgggcg cgggctccga cgtgggtagc
    47641 ctgaaaacga cttatacccg tagaaatggt aagatttatc ttaatggtag taagtgtttt
    47701 attaccagta gcgcctacac cccgtacatc gtggtgatgg cgcgcgacgg ggcttctccg
    47761 gacaaacctg tctacaccga atggtttgtt gatatgagca aaccgggcat caaagtgacc
    47821 aaacttgaga agctcggtct gcgtatggat agctgctgtg aaatcacctt tgacgatgtg
    47881 gaactggacg agaaagacat gttcggtcgg gaaggtaacg gctttaaccg cgtcaaagaa
    47941 gagttcgacc atgaacgttt cctggtagcc ctgaccaact acggtacggc gatgtgcgcc
    48001 tttgaagatg cggcgcgcta cgccaaccag cgcgtgcagt ttggcgaggc tattggtcgt
    48061 ttccagttga ttcaggaaaa attcgcccac atggcgatca aattaaactc catgaaaaac
    48121 atgctgtatg aagcagcgtg gaaagcagac aacggcacca tcacctctgg cgatgcagcg
    48181 atgtgcaaat acttctgcgc caatgcggca tttgaagtgg tggatagcgc aatgcaggtg
    48241 ctgggcggtg tcgggattgc gggcaaccac cgcatcagcc gcttctggcg tgacctgcgt
    48301 gtagaccgcg tttccggagg atctgacgaa atgcagatcc tgacgctggg tcgtgcggtg
    48361 ctgaagcaat accgctaagt ccgtgcgctg cctgatgcga cgctgacgcg tcttatcagg
    48421 cctacgggag cacgaatgta agccggataa ggcgtttacg ccgcatccgg cagtcatgcg
    48481 ctacataaat ttttcaggag ctaataatgg atcatctacc catgccgaaa ttcggaccgc
    48541 tggccggatt gcgcgttgtc ttctccggta tcgaaatcgc cggaccgttt gccggacaaa
    48601 tgttcgcaga atggggcgcg gaagttattt ggatcgagaa cgtcgcctgg gccgacacca
    48661 ttcgcgttca accgaactac ccgcagctct cccgccgcaa tttgcacgcg ctgtcgttaa
    48721 atattttcaa agatgaaggc cgcgaagcgt ttctgaaatt aatggaaacc accgatatct
    48781 tcatcgaagc cagtaaaggt ccggcctttg cccgtcgtgg cattaccgat gaagtactgt
    48841 ggcagcacaa cccgaaactg gttatcgctc acctgtccgg ttttggtcag tacggcaccg
    48901 aggagtacac caatcttccg gcctataaca ccatcgccca ggccttcagt ggttacctga
    48961 ttcagaacgg tgatgttgac cagccaatgc ctgccttccc gtataccgcc gattactttt
    49021 ctggcctgac cgccaccacg gcggcgctgg cagcactgca taaagtgcgt gaaaccggta
    49081 aaggcgaaag tatcgacatc gccatgtatg aagtgatgct gcgtatgggc cagtacttca
    49141 tgatggatta cttcaacggc ggcgaaatgt gcccgcgcat gagcaaaggt aaagatccct
    49201 actacgccgg ttgcggtctg tataaatgtg ccgacggcta catcgtgatg gaactggtgg
    49261 gcattaccca aattgaagag tgctttaaag atattggcct cgcacatcta ctcggtacgc
    49321 cggaaatccc ggaaggcact cagcttatcc accgtatcga atgcccttac ggcccactgg
    49381 tagaagaaaa actcgatgcc tggctggcgg cacataccat cgcagaagtt aaagaacgct
    49441 ttgccgaact gaatatcgcc tgtgccaaag tactgaccgt accggaactg gaaagcaatc
    49501 cacagtatgt tgcccgtgaa tcaatcactc agtggcaaac gatggatggt cgcacctgca
    49561 aagggccgaa catcatgccg aaattcaaaa ataaccccgg acaaatctgg cgcggaatgc
    49621 cctcacatgg catggacacg gctgccattt tgaaaaatat cggctacagc gaaaacgaca
    49681 ttcaggagtt ggtcagcaaa ggtctggcca aagttgagga ctaaacgcgt ggcgtcagtc
    49741 ggcttgcctg actagcgccc gcccaataac aaacgaacaa aaatggatag aggtgcaatg
    49801 gatatcattg gcggacaaca tctacgtcaa atgtgggacg atctggcgga cgtttacggt
    49861 cataaaacgg cgctgatttg tgaatccagc ggcggagtcg ttaaccggta tagttatctt
    49921 gagttaaatc aggagattaa ccgcacggca aacctgtttt atacgctggg gattcgcaaa
    49981 ggcaacaagg ttgcactaca tctcgacaac tgcccggaat ttatcttttg ctggttcggg
    50041 ctggcaaaaa ttggcgcgat tatggtgccg attaacgccc gcctgttacg cgaggaaagc
    50101 gagtggatct tgcaaaatag ccaggcgtgc ctgctggtga ccagtgcgca attttatcct
    50161 atgtatcaac agattcagca ggaagatgcc actcaattgc ggcacatttg cctgacagat
    50221 gtggcacttc ccgctgatga tggagtgagt tcatttactc aactgaaaaa tcaacaacct
    50281 gccaccttgt gctatgcacc gccgctatcg actgacgata cggcggaaat tctcttcacc
    50341 tccggcacca cctcccgacc gaaaggtgtg gtgattaccc attacaacct gcgcttcgct
    50401 ggatattact ccgcctggca gtgtgcactg cgtgacgatg acgtctacat gacggtaatg
    50461 cctgcgtttc atatcgattg ccagtgtact gcggcgatgg cggcgttttc tgccggggcc
    50521 acctttgtgc tggtcgagaa atacagcgcc cgcgccttct ggggacaggc gcagaagtac
    50581 cgcgccacca ttaccgaatg tattccgatg atgatccgta cgttgatggt gcagccgcct
    50641 tcagcgaacg atcggcaaca ccgcctgcgg gaagtgatgt tttatctcaa cttgtcggag
    50701 caggaaaaag acgcattttg tgaacgcttc ggcgttcgct tgctgacgtc ttatgggatg
    50761 acggaaacca ttgtgggcat tatcggcgat cgccctagcg ataaacgacg ctggccgtcg
    50821 attggtcggg cggggttttg ctacgaagcg gagatccgcg acgatcacaa tcgcccgctc
    50881 ccggcaggtg agatcggtga aatctgtatt aaaggcgtac cagggaaaac catcttcaaa
    50941 gagtattttc tcaacccgaa agccactgcg aaagtgctgg aagccgacgg ctggctacat
    51001 accggcgata ccggataccg cgacgaagaa ggcttttttt atttcgtcga tcgccgctgc
    51061 aatatgatca aacgcggtgg cgagaatgtc tcctgcgtgg agctggaaaa tatcatcgcc
    51121 acccatccga aaattcagga catcgtggtt gtgggtatta aagattcgat tcgcgatgaa
    51181 gccatcaaag catttgtggt gctgaatgaa ggtgaaacat tgagcgaaga ggaatttttt
    51241 cgcttctgcg aacaaaatat ggcgaaattt aaagtgccct cttatctgga gatcagaaaa
    51301 gatctgccac gtaattgctc ggggaaaata attagaaaga atctgaaata aatgaaacgt
    51361 cagggaacta ccctgccagc taataatcac gccctaaaac aatatgcatt ttttgcaggg
    51421 atgttgtcgt ccctaaaaaa gcaaaaatgg agaaaaggaa tgagtgaatc attacatctg
    51481 acccgcaatg gatcaattct ggaaattacc cttgatcgcc caaaagcgaa tgctattgat
    51541 gcaaaaacca gctttgaaat gggcgaagta tttctaaatt tccgtgacga tccgcaatta
    51601 cgtgtcgcca ttattaccgg tgccggagag aaattctttt ccgcgggctg ggatttaaaa
    51661 gcggcagcag aaggcgaagc accggatgct gactttggcc cgggtggttt tgcgggatta
    51721 accgaaattt tcaatctcga caaaccagtt atcgcagctg tgaacggcta cgcctttggc
    51781 ggcggtttcg aactggcgct ggcggcagat tttattgttt gtgccgataa cgccagcttc
    51841 gccctgccgg aagccaaact gggcatcgtt cctgacagcg gcggtgtgct gcgtctgccg
    51901 aagatcctgc cgcctaccat cgtcaatgaa atggtgatga ccggcagacg aatgggcgca
    51961 gaagaggcgc tgcgttgggg gatagtcaac cgcgtggtta gccaggcgga actgatggat
    52021 aacgcccgcg aactggctca gcagctggtt aacagcgccc cgctggcgat tgcggcgctg
    52081 aaagagatct accgcaccac cagcgaaatg ccggtagaag aagcgtatcg ctatattcgc
    52141 agcggcgtgt tgaaacacta tccatcggtt ctgcattcgg aagatgccat tgaagggccg
    52201 ctggcgtttg ccgagaagcg cgatccggtg tggaaaggac gttaacaacc gtgagctatt
    52261 acgcctttga ggggttaatt ccggtggttc acccgacggc gtttgtccat cccagtgccg
    52321 tcttgattgg cgatgtgatt gtgggagccg gtgtctacat cggcccactc gcctcactgc
    52381 gtggtgacta cgggcggttg atcgtgcaag cgggagccaa tattcaggat ggctgcatta
    52441 tgcatggcta ctgcgacact gacaccatcg ttggggaaaa cggccatatc gggcatggag
    52501 cgatcctgca tggttgtgtg attggtcgcg atgcattggt cgggatgaac agcgtaatta
    52561 tggatggcgc ggtcattggc gaagagagca ttgttgccgc aatgagcttt gtcaaagcag
    52621 gctttagtgg cgagaaacgc cagttgttga tgggtacgcc agcccgcgct gtacgcagtg
    52681 ttagtgacga cgagttacac tggaaacggt tgaataccaa agagtatcag gatcttgtcg
    52741 ggcgctgtca tgcatcgtta catgaaacgc agccactgag gcaaatggag gaaaatcgcc
    52801 cccgtttgca ggggacgacg gatgtgacgc ctaaacggtg atcttgccgg atgcgacctg
    52861 gttgctggat aagatgctac aacatcgtat ctggcaagca ggacgcccgg tatcaacgag
    52921 ataaaattaa cgacgcatac tctttgacaa catcatctgc cacttctgct cgcggttaag
    52981 ttccgtgagc ggaacggcag gaatacgcgg tgtctttctt acgccgacta atttttcccg
    53041 actgttattg cgcaggcgcg cgtaaatttg ctcatcgata tcgaccactt taaccagtcg
    53101 ctgacactgg catccccgcc cttccagctt attagggatc atcttaactt cgcagcttat
    53161 ttctgtgact tccgacagaa tataagtcag ggtattaacc gccttgctgt gttcaatatc
    53221 gaaatgaata gagatctctc ttgctatcac ccaccgattt tcagccatca tccattcggc
    53281 gattaacaag tagagtggtt tttcaacata tccttcacac atacgaactc cagtttcaca
    53341 aattaaaagt ctggctatga ctcgttttga agcaattaaa caagaccata ttaaaattgt
    53401 ggatatatcg atagtatgca acttcaccgt cgataagtgt gagctaaagc ttatgtcatc
    53461 aaaaatatcg attccccaaa agatttactt aatgggcaaa aaaaaacggt cctcatcaga
    53521 gaaccgtatt agttattcga tcaaattagc ggatgaaaaa tatccgccac gacacgctat
    53581 tatttgatct gtgcgtgcat ttcctgcacc gaaattactt tttcagtcgc atcggcattc
    53641 agcgccatcg cggtagcgaa accaccgttc agggtggtgt cataatgcac tttatattgc
    53701 agcgcactgc gacggatcac gcgggagtct tcaatcgcac gacggcctga ggtggtgttg
    53761 atgatgtagg tatattcgcc attcttgata cggtcctgaa tgtgcggacg gccttcatgc
    53821 accttgttta ccagacgcgg attgataccc gcttcgccca gcacaatcgc cgtgccgtgg
    53881 gtcgcatcca gctcgaagcc ctgtttcagc agttttgccg ccaggtccac cacgcgttct
    53941 ttatcgcctt cgcgcacgga aagcagcgca cgaccgtgtt tcttcatggt ggagttgctg
    54001 cccagctgcg ctttggcgaa cgcttcagcg aaggtgcggc ccacgcccat gacttccccg
    54061 gtagagcgca tttctggccc taacagcggg tcaacgcctg ggaatttatt gaacggcagc
    54121 accacttctt tcaccgagta gtacggcgga ataacttctt tggttacgcc ctgctcagcc
    54181 agcgatttgc cagccatcac acgcgccgcc acttttgcca gcggtacgcc ggtggctttg
    54241 gagacgaacg gaacagtacg cgccgcacgc gggtttactt caatcaggta aacttcgttg
    54301 tttttcaccg caaactgcac gttcatcaga ccgcgtacct gcaattcgaa ggccagtttc
    54361 tgcacctgct ggcgcatcac atcctgaatt tcctgactta aggtgtacgc tggcagcgaa
    54421 catgcggagt caccggagtg cacgcccgcc tgctcgatgt gctccatgat gccgccaatc
    54481 agcaccattt cgccgtcgca gatggcatcc acgtcaactt ctactgcgtc atcaaggaaa
    54541 tgatccagca acactggcgc atcgttagac acgctaaccg ccgtctggaa gtagcgacgc
    54601 aggtcagctt cgtcatagac gatttccatc gcccgaccgc cgagaacgta agacggacgc
    54661 accaccagcg ggtagccaat ctcttttgcc ttctcaactg ccatttcaat agcagtaacg
    54721 gtggcgttcg ccggttgttt cagtttcaga cggtcaaccg catgctggaa gcgttcacgg
    54781 tcttccgcac ggtcgatagc atccgggctg gtgccgataa ccggtacgcc agcggcttcc
    54841 agtgcacgcg ccagtttcag cggggtttga ccgccgtact gaacgataac gcctttcggc
    54901 ttctcaatac gcacgatttc cagcacatct tccagagtta ccggctcgaa gtagaggcga
    54961 tcggaagtgt cgtagtcggt agagacggtt tccgggttac agttaaccat aatggtttcg
    55021 taaccgtctt cacgcagcgc cagcgaggcg tgtacgcagc agtagtcgaa ttcgataccc
    55081 tgaccgatac ggtttggacc gccgccaagc accatgattt tttcacggtc ggtagacgga
    55141 ttcgcttcgc actcttcttc ataagtggag tacatgtaag cggtgtcggt ggcaaactct
    55201 gccgcacagg tatccacgcg cttgtagacc gggtgcaggt catattggtc acgcagctta
    55261 cggatttccg cttcgcgtac gcccgccagt tttgccaagc gcgcatcggc aaagcctttg
    55321 cgtttcagct ggcgcaggaa ttcagcgttc aggccagtga tgcccacttc cgccactttc
    55381 tcttccagac gcaccagttc ttcaatctgt accaggaacc agcggtcaat gttagtcagg
    55441 ttgaagacgc cgtccacaga caggcccgcg cggaaagcat cggcgatgta ccagatacgc
    55501 tctgcgcctg cgtctttcag ttcgcgacgg attttggtta acgcttccgg gtcatccagg
    55561 ctcactttcg ggtcgaagcc agttgcgccc acttccaggc cgcgcagcgc tttttgcagg
    55621 gattcctgct gtgtgcgacc aatcgccatc acttcgccaa ccgatttcat ctgagtggtc
    55681 agacggtcgt tagcaccggc gaatttttcg aagttgaagc gaggaatttt agtaaccaca
    55741 tagtcgatgg acggctcgaa ggaggccgga gtacgtccgc cagtgatgtc gttcatcagt
    55801 tcgtctaggg tgtaacccac cgccagtttc gccgccactt tagcaatcgg gaaaccggtc
    55861 gctttcgacg ccagcgccga agaacgggac acgcgtgggt tcatttcgat aacaatcagg
    55921 cgaccgtttt tcgggttcac cgcaaactgg acattggaac caccggtttc aacgccgatt
    55981 tcacgcagca ccgccatcga ggcgttacgc atgatttgat attctttgtc agtcagcgtt
    56041 tgggctggcg cgacagtgat ggagtcaccg gtgtggatgc ccatcgcatc aaagttttcg
    56101 atagagcaga cgatgatgca gttgtcgttt ttatcacgca ccacttccat ctcgtactct
    56161 ttccagccga tcagcgactc atcaatcagc aactctttgg tcggagagag atccagaccg
    56221 cgggcgcaaa tttcttcaaa ctcttcacgg ttataagcga taccgccgcc gctaccgccc
    56281 atggtaaagg atgggcgaat aatgcacggg aagcccacgt cagcggcaac agccagcgct
    56341 tcttccatcg tgtgtgcgat accggaacgc gcggtttcca gaccaatttt cttcatcgct
    56401 acgtcgaaac gacggcggtc ttctgcttta tcaatcgcat cggcagtggc accaatcatg
    56461 gtgacaccga actcttccaa cacgccctga cgttccagct ccagcgcgca gttcagcgcc
    56521 gtctgaccgc ccatggttgg cagcaccgcg tccgggcgct ctttttcaat aatcttgcgt
    56581 accacttccc agtgaatcgg ctcgatgtag gtcgcatcgg ccatttccgg gtcggtcatg
    56641 atggtcgccg ggttggagtt caccagaatg acgcggtaac cctcttcgcg cagggcttta
    56701 caggcttgcg cgccagagta gtcaaactca cacgcctgac cgataacaat cgggcccgca
    56761 cccagaatca ggatactttt tatatctgta cgttttggca tggctctttt actcctgatt
    56821 acttagcggt tttacggtac tgctcaatta acttgataaa gtggtcgaac aacggcgcgg
    56881 cgtcgtgtgg accagggctg gcttcagggt gaccctggaa gctgaacgcc ggtttatcga
    56941 tacgatgaat gccctgtaac gtaccgtcga acagggattt atgcgtgaca cgcaggtttg
    57001 caggtaatgt tgcttcatcc accgcaaaac cgtggttctg ggcggtgatc atcaccacgt
    57061 ttttctccac atctttaacc ggatggttgc cgccgtggtg accaaatttc attttgacag
    57121 tcttcgcacc gctcgccagc gccagcagct gatgaccgag acagatgccg aataccggaa
    57181 tatcggtttc gaggaacttc tggatggcgg taatggcgta atcgcacggg gccgggtcgc
    57241 caggaccgtt ggagaggaag atgccgtctg gattcatttt cagcacatct tccgcagaag
    57301 tttgcgccgg aacgatggtc aggcgacagc ctctatccac cagcatccgc aggatgttgc
    57361 gcttggcacc aaaatcataa gccacgacgt ggaacggcag ctcgtcttct tttttcgctt
    57421 ctggcaggcc gccggtcaac gtccagctcc cttgtgtcca gctataggct tctgcggtgg
    57481 tcacttcttt tgccagatcc atgccgttca gacctgggaa cgcgcgggct ttttctaacg
    57541 ccagcgccgc atccgggtta tcgcctgcga taatgcagcc attctgtgcg cctttctcgc
    57601 gcagtaaacg cgtcagctta cgggtatcga tatcggcaat cgccacgatg ttatggcgct
    57661 tcaggtaaga agagaggtct tcggtattac ggaagttgct ggcaatcagc ggcaggtcgc
    57721 gaatcaccag accttgtgca tgtacctgag aagattcttc atcggcgtca ttagtgccga
    57781 cattgccaat atggggataa gtaagagtaa cgatttgacg agaataggaa ggatcagtga
    57841 ggatttcttg ataaccggtc attgaagtat tgaaaacgac ttccccaacc gccgaacctg
    57901 ttgcccctat ggcccgaccg tgaaactggg ttccgtcttc cagaaccaat agcgctgact
    57961 taatcaaaac accctccata gaatattcac tcactttatt tgcatattaa ttcacagtga
    58021 tgacgtaaat caatgcaaat ctgcttaccg acgaatttct ggcaaacggc ggcattctgg
    58081 agatatgggc gctaaaagtc aacttaatgg caggattttt tatattattt tgtggctcat
    58141 gcatgtttta caggcattaa gcgacaaaaa catcatctaa cctgttctgg aaaacgcttg
    58201 cgcagcaaga agattgcatg aaataaaaac gagcaggaaa aagtggacca aatggtcaaa
    58261 atttacatta aaacaacaaa aatagaagac gaaacatgta atttttggca gttatagaaa
    58321 atttaaaagc ataacaccaa aaataaaagg gctaaaaatt aaagcccttt ataattaata
    58381 aattaaatgt gttacttttg catcataata aacattttgt ggttacaaat tattgagatc
    58441 aagcacatct cgcatatcaa aaagaccact ttccttacca ctcaaccaca aagccgatct
    58501 taccgcaccg ttagcaaatg tcatgcggct ggacgcctta tgggtgatct ccagacgctc
    58561 gccaatatcg gcaaacatcg cggtatgttc gccaacgatg tcacctgcac gcacggtggc
    58621 aaaaccaatg gtaccaggca cacgttcacc ggtatggcct tcacgactgt agaccgcgca
    58681 atctttcaga tctttatcaa gggcgtgggc gatcgcctct cccattgcca gtgcggtgcc
    58741 tgacggcgca tcaactttat gtctatgatg tgcttcaata atttcgatat cggtgtagtc
    58801 acccatcact ttggctgctt tctccagcag cttaagcatg acgttaacgc caacgctaaa
    58861 attggcggca aagacaatcg caatatcggc ggcggcgtca cgaattgcct gtttaccggc
    58921 ttcgtcaaac cccgtagtgc cgatcaccat ccctttgcca tgctgccgac aaaaagcgag
    58981 atggttcagc gtaccttccg gacgggtaaa atcgataaac acatcaaaat catcttttat
    59041 cgcatcaaga ctgctttgca cggtaacgcc tgttttccct gcaccagcca gctcaccggc
    59101 gtcgctgccc agtaaagaag atccttcgcg ctccagcgcc gcgcccagct gcacgccttc
    59161 taatgccagt gccgcctgaa tcaactggcg gcccatacgc cccccggctc ccgcgatggc
    59221 aacgcggatg tttgcatcat gcatagctat tctcttttgt taatttgcat agaccgtttt
    59281 cagagtaacc agcgcatcgc tacgccgcca gccaaaaacg ctgataatta gaaattaatg
    59341 ataaacaggg agggatatca gtgaaaggca tgagtcgatg aatgactgca tgccgataac
    59401 atgtgacagg ttacggcacc agagccagca cctcagccac ccactgttgg aagcctttta
    59461 cattcagatc caatgccacc tgtacattgg ctggtttgcc caggcaaccg tcgatatcaa
    59521 ccaccgtcgt gcctgaggta aattcgcctt gagtttctac cgcgacaaaa cacggcttga
    59581 gggtgaataa gtccgggcgc accagccagg cgatggcgca gagatcgtgc attcgcagac
    59641 cgctttgcat actgccgcta cggtagtggc taaacagggc gtggagcatt tttccggtgc
    59701 gattcaactc cggtaaagta gcgagatagt caggggttaa tattgcctga ttggtgacat
    59761 ccagaccgca catgacaatt tcgatgccgc tacggaagac acaggcagcc gcttctggat
    59821 cggcagcgat attaaactcg gcatttggtg tacagttgcc gcgaccagca gaaccgccca
    59881 tgatcaccag acgacgaata tacggtttac attccgggca ttgtaaaagt aacagcgcaa
    59941 tattggttaa cgggccgatg gccaccaggg taacgggctc tggtgcacgc atcagggcat
    60001 cccgaatcgc cagaaacgcc ggtatcccga gtggctttcg gttgtgctca acaaagtcgt
    60061 agccagccat tcccgattcg ccgtgcacag atgctgcatc acgcggtgcg cgtaccagcg
    60121 gcacagcggc tccctgggcg agcggaatct ccgcattcca gaaatgcagc agttgcaggg
    60181 cattgcgggt agttttctca accgagacat tacccgcgac ggtggtcatc agttgcaggt
    60241 cgagttcggg ggcaaaaatc gcggcggcaa tggcgacggc atcgtcaatg ccggggtcag
    60301 tatcgaggaa gataggtaaa cgcatgtttt cttcataaaa aatgccggta acaagaccgg
    60361 cattttcgca taacttaggc tgctaatgac ttaatcgact tcacgaatat cgacacgcag
    60421 ctctttcggc acttcgaaaa caatattttc ttcacggcct tccagcggaa tggcttcacc
    60481 accacccaac tgctgcaaac gtgccaccac attctgcacc agaatatccg gagccgatgc
    60541 gcccgcagtc acgccgacgc atttaacctc tttcacccac tcttcctgga tatctttcgc
    60601 atcgtcaatc aaaaacgcgt gtttgcccat acgctgggcc agctccgcca gacggttgga
    60661 gttggaggag tttttcgaac cgaccaccaa cacaacttcc gcctgttctg ccagggcgcg
    60721 taccgcttcc tgacggttag tcgtggcgta gcagatgtca tctttgcgcg gaccgacaat
    60781 tttcgggaag cgtttacgca gcgcgtcgat cacatcagac gtatcatcca ccgacagcgt
    60841 ggtttgggtc ataaaggaga gcttctcttc gtttttgacc gtcagtttcc acacatcgtc
    60901 tggcgattcg accagataca ttcccccttc ggggttgctg tactgcccca tcgtcccttc
    60961 cacttccggg tgcccggcgt ggccgatgag aatagattct tcgccacggc gactggcgcg
    61021 ggcaacttcc atatgcactt tggtcaccag cggacaggtg gcatcaaaca ccgtcaaatc
    61081 gcggcttttc gcttcgttac gaaccgcctg agaaacaccg tgtgcggaga aaatcaggat
    61141 cgcgccgtcc ggcacttcgc taatctgctc aataaagata gccccacgct cgcgcaagct
    61201 atcgaccacg tagcggttat gcaccacttc gtgacggaca tatatcggtg cgccgtaaat
    61261 ggccagcgcg ttttcaacaa tgctgatagc gcggtctacc ccggcacaaa aaccacgcgg
    61321 gttggccaac aggatctgca tgttacgcct ccagtgccgg atcgatttcc agcacttcaa
    61381 tatcaaaatg aacggtctgc ccggccagcg gatggttgaa atcaacggta atggagtcgc
    61441 cgttaatttc gcggatcaca ccaggcatct cactgccatc cattgcggta aaaagcatga
    61501 ttgcgccaat ttctggctcg cctgcatcca taaattcacg gcgggagaag tactgaatca
    61561 ggtccggtga cggcacgcca aacgccgcat ctggctccag cgagaaggtg gttttgtcgc
    61621 ccactttcag ccccagcagg tgttgctcca gcccttcaga aagagaagca tcgcccaggc
    61681 ggaacagcgc cggtttaccg ttgttgcgag tagactcggc ggtggtgcca tcgtcaagtt
    61741 ttagcgtgaa gtgcaccagg acggcgctat tgctctgtac agattcagac atgcaggttt
    61801 gctcttattt ttgaggattt ttgccggatg cggcctacga tttcgtagca atctgtaggc
    61861 cgcatccggc agggtttatt attgtttttt cgctttggaa ggtaaaaacc cttccagcac
    61921 gatcagtgcc gcaccgacac aaatggcagt atcggcaagg ttgaaggtgg cgaagtgcca
    61981 gtcgccgacg tagaagtcga tcatatcgac aacgaagccg tgccacaggc ggtcgaacag
    62041 gttgcccagc gcgccgccaa taatcagcgc gtaagcgata ttgtttagct tctgcgtggc
    62101 cttcgagcga tacatcatca ctgccaggag cacgctaata ccaatcgcaa taccggcaaa
    62161 gaaccaacgc tgccagccgc cgctatcggc aaggaaacta aacgccgcgc catagttacg
    62221 cgcataatgc agattaagcg acgggaacag cgggaccgta tcccccagag caaagttctg
    62281 gaggatcagg tatttgctgc ccagatcgat aatcagcacg actaccacca gccacagcca
    62341 gcgtagccct gttgaacaga tcgattgact catcaggcaa acttacgttt ttcaccgtca
    62401 ccggcgacgt tgctgacaca gcggccgcag atttctgcgt gttccgccac cttgccgaca
    62461 tcctgggtgt agtgccagca gcgtgggcac ttctcacctt cggctttact caacgcgact
    62521 ttcagccctt tgagtacttc gctctgctga gcatcagcag gtgcgtcgtt atagtctgca
    62581 acggtagcgc cggaggtcaa caggacaaat cgtaattcat cgcccagcgc ggtcagtttc
    62641 gccgccagtt ccggttctgc atacaaggtt actgccgctt ccagcgagcc acccactttc
    62701 ttgtcggcac gcgcttgctc aatgactttg ttcacttcgc cacgcacttt caacagctcg
    62761 tcccagaacg catcgttcat tgcttcactg tctgccagac caaacaggcc ttcgtaccac
    62821 tcaccggtga agacgtattt ttcacgttcg cccggcagat agccccacac ttcatcagcg
    62881 gtgaaggaga ggattggtgc catccagcgc accagcgctt cagcgatgtg atacagcgca
    62941 gtctggcagc tacgacgcgc cacgctatcc gctttggcgg tgtactgacg gtctttgatg
    63001 atgtcgaggt agaaggaacc catctcaacg gagcagaagc gcatcagacg ctgtaccact
    63061 tcgtggaaat cgtatgcttc gtacgccttg aggatgtctt cctgtgccgc tttcgcacaa
    63121 cctacggccc agcgatccag taccaccatc tcttccggtt tcaccatatc ttttgctgga
    63181 tcaaaaccat tcaggtttgc cagcaggaag cgcgcggtgt tacggatacg acgatagcta
    63241 tcggcagcac gtttcaggat ctcgtcagaa acggccattt caccggtgta gtcggttgat
    63301 gccacccaca gacgcagaat atccgcgccc agtttgttca tcacatcctg cggcgaaacg
    63361 gtgttgccga tggatttaga catcttgcga ccctgaccat ccacggtaaa gccgtgggtc
    63421 agtacctgac gatacggcgc tttgcctttc atcgcggtgg agatcattag ggaagacatg
    63481 aaccaaccac ggtgctggtc agaaccttcc agatacatgt ccgctgcgtg accggcaaat
    63541 tccggacgca cgtcaacaac agaagagtgg gtagatccgg agtcaaacca tacgtcgaga
    63601 gtatccggca ctttcacata ctggtcagct tcgtcgccga ggatctcttt cgcatcgaga
    63661 tcccaccacg cctggatgcc gtcaacttca acgcgtttag ccacttcttc catcagttcg
    63721 agggtacgcg gatgcagctc ttccgtgtct ttgtgcacga acagtgacat cggtacgccc
    63781 caggtgcgct gacgggagat acaccagtca ggacggttag caaccatcga ctcgatacgc
    63841 gcctggcccc agtccgggat ccactgcacg cctttgatct ctttcagtga ctgcgcacgc
    63901 agacctttct gatccatgct gacgaaccac tgcggtgtcg cgcggaagat gatcggcgtt
    63961 ttgtgacgcc agcagcacgg atagctgtgc tgcattttct caacgtgcag cagcgcgcct
    64021 ttttcctgca gcagcgcaac gacgatgtcg ttcgctttga agacgttcac gccatccagc
    64081 gtcggataag tgcccggcag ataagtgccg tccgggccaa ccgggttagc ggtttccagg
    64141 ccgtatttct gaccgatcac atagtcgtcc gggccgtggc caggcgcggt gtgaacggca
    64201 ccggtaccgg catccagggt aacgtgatcg ccgaggattg ccggaacgtc gaagcccata
    64261 aacggatggg taaagcgcag cagctcaagc tctgcacctt ttaccgtgcc gagaatggtg
    64321 taatcggtca cgccgatacg ctgcattacg ctttcaacca gatctttcgc cagaatcgcg
    64381 gcctgaccgt cgatctgcac cagcgcatag tcgaaatcag gtgcaataga gattgcgcgg
    64441 ttcgcaggca gagtccacgg cgtagtggtc cagatcacca gcgagattgg gccgttaacg
    64501 ttgcttacgc caaatttcgc tttcagcgca tcctgatcga ccgcctggaa agcgacgtca
    64561 atggacggag aagttttgtc gtaatactca acttccgctt ctgccagtgc agaacggcag
    64621 tctacgcacc agtgcaccgg cttcgcgcct ttgtgcaggt gaccattgcc gatgattttg
    64681 cccagcgcgc ggatgatgtt ggcttcagtt ttgaagtcca tagtcagata cgggtgcgac
    64741 cagtcgccca gcacgcccag acggataaag tctttgcgtt gaccgtcaac ctgggtcgcc
    64801 gcgtattcgc ggcacttggc gcggaactcg gcggcggtga atttctcacc cggcttaccg
    64861 tattcttgct ctactttcag ctcgatcggc agaccgtggc agtcccagcc aggcacatac
    64921 ggcgagtcat aaccggaaag ccctttggac ttcacgataa tgtctttcag aatcttgtta
    64981 accgagtgac caatatgaat gctgccattc gcataaggag ggccatcatg cagaatgaag
    65041 gtttttttgc cttttttagc cgcacggatg atgccgtaca gatcatcatc agtccaacgc
    65101 gccagcattc ccggttcgcg cttggcgaga tcgccacgca tcgggaaccc tgtttccggc
    65161 aaattcaggg ttgatttata gtcactcatc agattctcgg ttccgtgttt cggtttgatt
    65221 acataacagg cttaagccgg ttttgttagc ccaaaaaatt cgcgggcggt taattcatca
    65281 cgcgcaatct gcgctttcag ttcgtccagc gacgcaaatc gctgctcatt gcgtattttt
    65341 ttacgcagca ctacttgtat atggcgaccg taaaggtcca ttgcaacatc taacaaatgc
    65401 acttccagct gctggcgaat accggcaacc gttgggcgtg ttccgatgtt tgccacgccg
    65461 ggtaacggct tttcaccgag gcccagcact tctaccgcat aaaccccttt caccggggaa
    65521 acctgacggc gtagcggtac attcgccgtc gggaaaccta tagtgcgccc taattcatca
    65581 ccgtggacta cacgcccgga gatggcaaac gggtgcccca gtaaactttc tgccagagcc
    65641 agattgtcat cagccagcgc ctgacgcacg gcggtgctgc tgatacgcac accaccttcg
    65701 caaaaagttt gcgtactggt gatatcgaag ccgtattcca tgccagcttt ctgtaataac
    65761 aagaaatcgc cttcacgacc agcgccaaag cggaaatcat cacctacggc aagaaatttt
    65821 acgcgcaaat gcttcaccaa aagatcgctg atgaaatttt gcgcggttaa cgccgcaaaa
    65881 cgcctgtcga aacgcacgca cagcacgtaa tcaacgccac actctgcaag gtaacgcagt
    65941 ttttcccgca gccgggtcag tcttgccggg gctttatcgg tagcaaacag ttccagtggt
    66001 tgaggttcaa aaagcatcac catcaccggt aagttgcgct tgcgcccttc ttcctgcaag
    66061 ccctgtaaca gcgcgcgatg accgcgatgc acgccgtcga aattaccaat agtcagcaca
    66121 cacccttctt gcggggcctg gctgagatta tgtatgccgc gtatcagctt catgtctggc
    66181 tcaaaacagt gaaaatcgtc cgagtatacc ttgtacagcg gtcaaggtta accggcgatt
    66241 gagtaccgaa atcagaaaga agtggctttt cattgccatg gcgcaaatca cgggaagaaa
    66301 ctgaccgcct gctgcaattt ttatcgcgga aaagctgtat tcacaccccg caagctggta
    66361 gaatcctgcg ccatcactac gtaacgagtg ccggcacatt aacggcgctt atttgcacaa
    66421 atccattgac aaaagaaggc taaaagggca tattcctcgg cctttgaatt gtccatatag
    66481 aacacatttg ggagttggac cttggctaat atcaaatcag ctaagaagcg cgccattcag
    66541 tctgaaaagg ctcgtaagca caacgcaagc cgtcgctcta tgatgcgtac tttcatcaag
    66601 aaagtatacg cagctatcga agctggcgac aaagctgctg cacagaaagc atttaacgaa
    66661 atgcaaccga tcgtggaccg tcaggctgct aaaggtctga tccacaaaaa caaagctgca
    66721 cgtcataagg ctaacctgac tgcacagatc aacaaactgg cttaatcgcc aatttgctga
    66781 agctttgtga aaaagcccgc gcaagcgggt ttttttatgc ctgctgcttt tgtgatgcgt
    66841 gagagaacac actgccggtt gccagatagt caaaagagac atgccgttgt actatctgaa
    66901 ctcttcgtgg cttaagaatc gtagaaccat cggctgatac attctgaaca agaatatcta
    66961 ctcaatctcc agactccccc acaaagaata tggatattgt gatacacatt gaggtattaa
    67021 acttaagacc actctatttt tgaattaaat tcctatatac tttaaccatt cataaatatc
    67081 tcctcattag acctt
//
LOCUS       DAETPZ010000009        65502 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.009, whole genome shotgun sequence.
ACCESSION   DAETPZ010000009 DAETPZ010000000
VERSION     DAETPZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65502
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.009"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            144..473
                     /locus_tag="K0W59_000681"
     CDS             144..473
                     /locus_tag="K0W59_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBI3021838.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            721..966
                     /gene="nrdH"
                     /locus_tag="K0W59_000682"
     CDS             721..966
                     /gene="nrdH"
                     /locus_tag="K0W59_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBI3021839.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            963..1373
                     /gene="nrdI"
                     /locus_tag="K0W59_000683"
     CDS             963..1373
                     /gene="nrdI"
                     /locus_tag="K0W59_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBI3021840.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            1346..3490
                     /gene="nrdE"
                     /locus_tag="K0W59_000684"
     CDS             1346..3490
                     /gene="nrdE"
                     /locus_tag="K0W59_000684"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBI3021841.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLQEAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            3500..4459
                     /gene="nrdF"
                     /locus_tag="K0W59_000685"
     CDS             3500..4459
                     /gene="nrdF"
                     /locus_tag="K0W59_000685"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBI3021842.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            4814..6016
                     /gene="proV"
                     /locus_tag="K0W59_000686"
     CDS             4814..6016
                     /gene="proV"
                     /locus_tag="K0W59_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HBI3021843.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            6009..7073
                     /gene="proW"
                     /locus_tag="K0W59_000687"
     CDS             6009..7073
                     /gene="proW"
                     /locus_tag="K0W59_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HBI3021844.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGACSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            7131..8123
                     /gene="proX"
                     /locus_tag="K0W59_000688"
     CDS             7131..8123
                     /gene="proX"
                     /locus_tag="K0W59_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HBI3021845.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            8315..9494
                     /locus_tag="K0W59_000689"
                     /pseudo
     CDS             8315..9494
                     /locus_tag="K0W59_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            9618..10355
                     /gene="ygaZ"
                     /locus_tag="K0W59_000690"
     CDS             9618..10355
                     /gene="ygaZ"
                     /locus_tag="K0W59_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HBI3021846.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            10345..10680
                     /gene="ygaH"
                     /locus_tag="K0W59_000691"
     CDS             10345..10680
                     /gene="ygaH"
                     /locus_tag="K0W59_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HBI3021847.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            10771..11301
                     /gene="emrR"
                     /locus_tag="K0W59_000692"
     CDS             10771..11301
                     /gene="emrR"
                     /locus_tag="K0W59_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HBI3021848.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMN"
     gene            11428..12600
                     /gene="emrA"
                     /locus_tag="K0W59_000693"
                     /pseudo
     CDS             11428..12600
                     /gene="emrA"
                     /locus_tag="K0W59_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            12617..14155
                     /gene="emrB"
                     /locus_tag="K0W59_000694"
     CDS             12617..14155
                     /gene="emrB"
                     /locus_tag="K0W59_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HBI3021849.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(14219..14734)
                     /gene="luxS"
                     /locus_tag="K0W59_000695"
     CDS             complement(14219..14734)
                     /gene="luxS"
                     /locus_tag="K0W59_000695"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBI3021850.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(14884..16440)
                     /gene="gshA"
                     /locus_tag="K0W59_000696"
     CDS             complement(14884..16440)
                     /gene="gshA"
                     /locus_tag="K0W59_000696"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBI3021851.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFHDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(16513..16941)
                     /locus_tag="K0W59_000697"
     CDS             complement(16513..16941)
                     /locus_tag="K0W59_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI3021852.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(16938..17504)
                     /gene="yqaB"
                     /locus_tag="K0W59_000698"
     CDS             complement(16938..17504)
                     /gene="yqaB"
                     /locus_tag="K0W59_000698"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HBI3021853.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVHPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(17652..17728)
                     /locus_tag="K0W59_000699"
     tRNA            complement(17652..17728)
                     /locus_tag="K0W59_000699"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17692..17694),aa:Arg,seq:acg)
     gene            complement(17793..17869)
                     /locus_tag="K0W59_000700"
     tRNA            complement(17793..17869)
                     /locus_tag="K0W59_000700"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17833..17835),aa:Arg,seq:acg)
     gene            complement(17934..18010)
                     /locus_tag="K0W59_000701"
     tRNA            complement(17934..18010)
                     /locus_tag="K0W59_000701"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17974..17976),aa:Arg,seq:acg)
     gene            complement(18014..18106)
                     /locus_tag="K0W59_000702"
     tRNA            complement(18014..18106)
                     /locus_tag="K0W59_000702"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18070..18072),aa:Ser,seq:gct)
     gene            complement(18422..18607)
                     /gene="csrA"
                     /locus_tag="K0W59_000703"
     CDS             complement(18422..18607)
                     /gene="csrA"
                     /locus_tag="K0W59_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBI3021854.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(18842..21472)
                     /gene="alaS"
                     /locus_tag="K0W59_000704"
     CDS             complement(18842..21472)
                     /gene="alaS"
                     /locus_tag="K0W59_000704"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBI3021855.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(21600..22100)
                     /gene="recX"
                     /locus_tag="K0W59_000705"
     CDS             complement(21600..22100)
                     /gene="recX"
                     /locus_tag="K0W59_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBI3021856.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(22343..23404)
                     /gene="recA"
                     /locus_tag="K0W59_000706"
     CDS             complement(22343..23404)
                     /gene="recA"
                     /locus_tag="K0W59_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBI3021857.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(23484..23981)
                     /gene="pncC"
                     /locus_tag="K0W59_000707"
     CDS             complement(23484..23981)
                     /gene="pncC"
                     /locus_tag="K0W59_000707"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HBI3021858.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYVLQTLWQQ
                     FLQNT"
     gene            complement(24126..25211)
                     /gene="mltB"
                     /locus_tag="K0W59_000708"
     CDS             complement(24126..25211)
                     /gene="mltB"
                     /locus_tag="K0W59_000708"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HBI3021859.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            25467..26030
                     /gene="srlA"
                     /locus_tag="K0W59_000709"
                     /gene_synonym="gutA"
     CDS             25467..26030
                     /gene="srlA"
                     /locus_tag="K0W59_000709"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBI3021860.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            26027..26986
                     /gene="srlE"
                     /locus_tag="K0W59_000710"
                     /gene_synonym="gutE"
     CDS             26027..26986
                     /gene="srlE"
                     /locus_tag="K0W59_000710"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBI3021861.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            26997..27368
                     /gene="srlB"
                     /locus_tag="K0W59_000711"
                     /gene_synonym="gutB"
     CDS             26997..27368
                     /gene="srlB"
                     /locus_tag="K0W59_000711"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBI3021862.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            27372..28151
                     /gene="srlD"
                     /locus_tag="K0W59_000712"
     CDS             27372..28151
                     /gene="srlD"
                     /locus_tag="K0W59_000712"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBI3021863.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            28256..28615
                     /gene="gutM"
                     /locus_tag="K0W59_000713"
     CDS             28256..28615
                     /gene="gutM"
                     /locus_tag="K0W59_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBI3021864.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGHFKPRVVVAIALDDQQCIVDILFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            28682..29455
                     /gene="srlR"
                     /locus_tag="K0W59_000714"
                     /gene_synonym="gutR"
     CDS             28682..29455
                     /gene="srlR"
                     /locus_tag="K0W59_000714"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HBI3021865.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            29448..30413
                     /gene="gutQ"
                     /locus_tag="K0W59_000715"
     CDS             29448..30413
                     /gene="gutQ"
                     /locus_tag="K0W59_000715"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HBI3021866.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(30410..31924)
                     /gene="norR"
                     /locus_tag="K0W59_000716"
     CDS             complement(30410..31924)
                     /gene="norR"
                     /locus_tag="K0W59_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HBI3021867.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRVVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            32111..33550
                     /gene="norV"
                     /locus_tag="K0W59_000717"
     CDS             32111..33550
                     /gene="norV"
                     /locus_tag="K0W59_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HBI3021868.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            33547..34680
                     /gene="norW"
                     /locus_tag="K0W59_000718"
     CDS             33547..34680
                     /gene="norW"
                     /locus_tag="K0W59_000718"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HBI3021869.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(34808..37060)
                     /gene="hypF"
                     /locus_tag="K0W59_000719"
     CDS             complement(34808..37060)
                     /gene="hypF"
                     /locus_tag="K0W59_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HBI3021870.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(37213..37740)
                     /gene="hydN"
                     /locus_tag="K0W59_000720"
     CDS             complement(37213..37740)
                     /gene="hydN"
                     /locus_tag="K0W59_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HBI3021871.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(37889..38899)
                     /gene="ascG"
                     /locus_tag="K0W59_000721"
     CDS             complement(37889..38899)
                     /gene="ascG"
                     /locus_tag="K0W59_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HBI3021872.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            39159..40616
                     /gene="ascF"
                     /locus_tag="K0W59_000722"
     CDS             39159..40616
                     /gene="ascF"
                     /locus_tag="K0W59_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HBI3021873.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVPWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQSVQPTVAKEVSLN"
     gene            40625..42049
                     /gene="ascB"
                     /locus_tag="K0W59_000723"
     CDS             40625..42049
                     /gene="ascB"
                     /locus_tag="K0W59_000723"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HBI3021874.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQDD
                     EITPNQQGIAFYRPVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFHEKGVTIDKALGDDEILKNTVDFVSFSYYTSRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(42164..42583)
                     /locus_tag="K0W59_000724"
     CDS             complement(42164..42583)
                     /locus_tag="K0W59_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3021875.1"
                     /translation="MTANAAHAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(42580..42891)
                     /locus_tag="K0W59_000725"
     CDS             complement(42580..42891)
                     /locus_tag="K0W59_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HBI3021876.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLGVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(43064..43807)
                     /locus_tag="K0W59_000726"
     CDS             complement(43064..43807)
                     /locus_tag="K0W59_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3021877.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFDKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            complement(43859..44329)
                     /gene="hycI"
                     /locus_tag="K0W59_000727"
     CDS             complement(43859..44329)
                     /gene="hycI"
                     /locus_tag="K0W59_000727"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HBI3021878.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(44322..44732)
                     /gene="hycH"
                     /locus_tag="K0W59_000728"
     CDS             complement(44322..44732)
                     /gene="hycH"
                     /locus_tag="K0W59_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HBI3021879.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARHKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(44729..45496)
                     /gene="hycG"
                     /locus_tag="K0W59_000729"
     CDS             complement(44729..45496)
                     /gene="hycG"
                     /locus_tag="K0W59_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HBI3021880.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGILHDLYCVWGGTDKIVPVDVYIPSCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(45496..46038)
                     /gene="hycF"
                     /locus_tag="K0W59_000730"
     CDS             complement(45496..46038)
                     /gene="hycF"
                     /locus_tag="K0W59_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HBI3021881.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(46048..47733)
                     /gene="hycE"
                     /locus_tag="K0W59_000731"
     CDS             complement(46048..47733)
                     /gene="hycE"
                     /locus_tag="K0W59_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HBI3021882.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDDWPDELYPLRKDSMDYRQRPAPTTDAETY
                     EFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAET
                     RMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSH
                     LLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRHDLLKDDM
                     IQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIACDFSNVGPMVRASGLA
                     RDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLM
                     VEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGN
                     TVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(47751..48647)
                     /gene="hycD"
                     /locus_tag="K0W59_000732"
     CDS             complement(47751..48647)
                     /gene="hycD"
                     /locus_tag="K0W59_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HBI3021883.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIGSPLPQLGDLITLLYLFAIARFFF
                     AISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNITDTVYHWPL
                     SQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLK
                     QLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDI
                     TPRITWAGFGFAFLAFVSLLAA"
     gene            complement(48650..50539)
                     /gene="hycC"
                     /locus_tag="K0W59_000733"
     CDS             complement(48650..50539)
                     /gene="hycC"
                     /locus_tag="K0W59_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HBI3021884.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGSVGVSGAMSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGSDIWLLGVIGFGLLAGI
                     IPLHGWVPQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMI
                     TAFVGGLYALMEHNIQRLLAYHTLENIGIILLGLGLGLGLGLGLGLGLGAGVTGIALE
                     QPALIALGLVGGLYHLLNHSLFKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIA
                     MLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVM
                     CMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAV
                     PLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSM
                     VITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVII
                     VSRGA"
     gene            complement(50536..51147)
                     /gene="hycB"
                     /locus_tag="K0W59_000734"
     CDS             complement(50536..51147)
                     /gene="hycB"
                     /locus_tag="K0W59_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HBI3021885.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPLLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(51272..51733)
                     /gene="hycA"
                     /locus_tag="K0W59_000735"
     CDS             complement(51272..51733)
                     /gene="hycA"
                     /locus_tag="K0W59_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HBI3021886.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            51945..52295
                     /gene="hypA"
                     /locus_tag="K0W59_000736"
     CDS             51945..52295
                     /gene="hypA"
                     /locus_tag="K0W59_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBI3021887.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            52299..53171
                     /gene="hypB"
                     /locus_tag="K0W59_000737"
     CDS             52299..53171
                     /gene="hypB"
                     /locus_tag="K0W59_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HBI3021888.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            53162..53434
                     /gene="hypC"
                     /locus_tag="K0W59_000738"
     CDS             53162..53434
                     /gene="hypC"
                     /locus_tag="K0W59_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HBI3021889.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVSFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            53434..54555
                     /gene="hypD"
                     /locus_tag="K0W59_000739"
     CDS             53434..54555
                     /gene="hypD"
                     /locus_tag="K0W59_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HBI3021890.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            54552..55562
                     /gene="hypE"
                     /locus_tag="K0W59_000740"
     CDS             54552..55562
                     /gene="hypE"
                     /locus_tag="K0W59_000740"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HBI3021891.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAAVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            55636..57714
                     /gene="flhA"
                     /locus_tag="K0W59_000741"
     CDS             55636..57714
                     /gene="flhA"
                     /locus_tag="K0W59_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HBI3021892.1"
                     /translation="MSYTPMSGLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(57751..58104)
                     /locus_tag="K0W59_000742"
     CDS             complement(57751..58104)
                     /locus_tag="K0W59_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HBI3021893.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(58136..58315)
                     /locus_tag="K0W59_000743"
     CDS             complement(58136..58315)
                     /locus_tag="K0W59_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3021894.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMFMKQVRKIVSIG
                     EPFFFRVIVPFKIKM"
     gene            58391..60952
                     /gene="mutS"
                     /locus_tag="K0W59_000744"
     CDS             58391..60952
                     /gene="mutS"
                     /locus_tag="K0W59_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBI3021895.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            61058..61710
                     /gene="pphB"
                     /locus_tag="K0W59_000745"
                     /pseudo
     CDS             61058..61710
                     /gene="pphB"
                     /locus_tag="K0W59_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(61761..62528)
                     /locus_tag="K0W59_000746"
     CDS             complement(61761..62528)
                     /locus_tag="K0W59_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBI3021896.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            62724..63632
                     /locus_tag="K0W59_000747"
     CDS             62724..63632
                     /locus_tag="K0W59_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBI3021897.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            63629..64891
                     /locus_tag="K0W59_000748"
                     /pseudo
     CDS             63629..64891
                     /locus_tag="K0W59_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
     gene            64888..>65502
                     /locus_tag="K0W59_000749"
     CDS             64888..>65502
                     /locus_tag="K0W59_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBI3021898.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAG"
BASE COUNT        15500 a        17654 c        16957 g        15391 t
ORIGIN      
        1 ctcgttattt tcaaccgcgg gcctacctta cctgattgcg cattcacaac gctttcagaa
       61 aagtccataa agccgcgatg aacctcagtt aagaaaatta tggtctacac tgaaaattac
      121 atcgaattct aatgggggat catatgttta accgaccgaa ccgcaacgac gttgatgatg
      181 gcgtgcagga tatccagaat gatgtcaatc aattagctga cagcctcgaa tctgtattga
      241 agtcctgggg cagcgacgcc aaaggggaag ctgaagccgc acgcagtaaa gctcaggcgt
      301 tgctgaaaga aaccagggcg cgaatgcatg gtcgtactcg cgtccagcaa gccgcgcgcg
      361 atgccgttgg ctgcgcggat tcttttgttc gtgaaagacc ctggtgtagc gtgggtacag
      421 cagctgcggt aggtattttt atcggcgcac tgttaagcat gcgcaaatcg tagtgcaaaa
      481 atgataataa atacgcgtct ttgaccccga agcctgtctt cggggtttct ttttgcctgg
      541 tgaatcacaa aaatccccct cccccgtcac gctcatatcc agggtaattt cgatcactat
      601 ttgctatata ttgtgtggtt gaatcttttt tcaactacat ctagtatctc tgtatcaaca
      661 gagagacaac ccgacgcgta tcatcgcgcc gtatcttcat tttaaacaga aatacgaatc
      721 atgcgcataa ctatttatac tcgtaacgat tgcgttcagt gccacgccac caaacgggcg
      781 atggaaaacc ggggctttga ttttgaaatg attaatgtcg atcgcgttcc tgaagcagcg
      841 gaagcgttgc gtgctcaggg ctttcgtcag ttgccggtag tgattgctgg cgatcttagc
      901 tggtctggtt tccgtccgga catgattaac cgtctgcatc cagcgccaca cgcggccagt
      961 gcatgagcca gctcgtctac ttctccagca gctccgaaaa cacgcagcgt tttatcgaac
     1021 gtttaggtct gcccgcggtg cgcatcccgc tcaatgagcg ggaacggatt caggtagacg
     1081 agccttacat cctgatcgtg ccctcttacg gcggcggcgg tacggctggc gcggtgccac
     1141 gacaggtaat tcgcttttta aacgacgagc acaaccgggc gttgcttcgc ggcgttattg
     1201 cttctggtaa tcgcaacttt ggtgaggcgt atggccgcgc cggagatgtg attgcccgga
     1261 aatgcggcgt gccgtggctg taccgttttg aactcatggg tacgcaaagc gatatcgaaa
     1321 acgttcgtaa aggagtaacc gaattttggc aacgacaacc gcagaatgcc tgacgcagga
     1381 aacgatggat taccacgcgc tgaatgcgat gcttaatctt tacgatagcg caggtcgcat
     1441 tcagttcgat aaagaccgcc aggccgttga cgcctttatt gcgacgcatg tgcgtccgaa
     1501 cagtgtgacc ttcagtagcc agcagcagcg cctgaactgg ctggtcaacg aaggttacta
     1561 tgatgaaagc gttcttaatc gctactctcg cgactttgtc attacgctgt ttgcccacgc
     1621 acacaccagc ggttttcgtt tccagacatt cctcggggca tggaagtttt acaccagcta
     1681 tacgttgaag acattcgacg gtaaacgtta tctggaagat tttgccgatc gagtcacgat
     1741 ggtggcgctg acgctggcac aaggcgatga gacgctggcg ttgcaactga ccgatgaaat
     1801 gctgtcagga cgctttcagc cagccacgcc aacattcctc aactgcggta agcagcagcg
     1861 cggcgaactg gtttcctgtt ttttgctgcg tattgaagac aatatggagt cgattggtcg
     1921 ggcggtaaat tccgcactgc agctgtcgaa acgcggcggc ggcgtagcat ttttgctgtc
     1981 gaatctgcag gaagcgggcg cgccaattaa acgtattgaa aatcaatctt ctggcgtaat
     2041 tccggtgatg aaaatgttgg aagacgcatt ttcctatgcc aaccaactcg gcgcgcgtca
     2101 gggggcaggt gcagtctatt tacatgctca tcatcccgat attctgcgtt ttctcgacac
     2161 gaaacgggaa aatgccgacg aaaaaatccg cattaaaaca ctgtcgctgg gcgtggtgat
     2221 cccggatatc actttccatc tggcaaaaga gaatgcgcag atggcgctgt tttcgcccta
     2281 tgacgttgag cgagtttatg gcaagccgtt tgccgatatc gccattagcg aacactatga
     2341 cgaactggtt gccgatgaac gcatccgcaa aaaatacctc aacgcccgtg atttcttcca
     2401 gcgactggca gaaatccagt ttgagtccgg ctatccctac atcatgtatg aagacacagt
     2461 aaaccgtgct aaccctatcg ccgggcgcat aaatatgagt aatctctgct cagaaatttt
     2521 gcaggttaac agcgcctcag agtatgacga gaatctcgac tatgcccgca caggccatga
     2581 tatttcctgc aatttaggtt cgttgaatat tgcgcacacc atggattccc ccgatttttc
     2641 ccgcacggta gagactgccg tgcgcggttt aacggcggtg tcagatatga gtcatatccg
     2701 cagcgtgccg tccatcgaag ccggaaatgc cgcctcgcac gccatcggac tggggcagat
     2761 gaatttacat ggttatctgg cgagggaagg catcgcttat ggttcgccgg aagccctgga
     2821 tttcaccaat ctctatttct ataccatcac ctggcacgca ctgcgtacct cgatgttgct
     2881 ggcgcgcgaa cgcggtgaaa ccttcgccgg gttcaaacag tcacgctatg ccagtggtga
     2941 atatttcagc caatatctgc aaggtaactg gcagccgaaa acggcgaaag ttggcgaact
     3001 gtttgcccgc agcggtatta cgttacctac ccgtgagatg tgggcgcaac tgcgcgacga
     3061 cgtgatgcgc tacggcatat acaaccagaa tctccaggcg gtgccgccaa ccgggtctat
     3121 ctcttatatc aaccatgcta cgtcgagtat tcatccgatt gtggcgaaag tagagatacg
     3181 caaagagggc aaaacaggac gcgtttacta ccctgccccg tttatgacta acgaaaatct
     3241 ggcgctgtat caggacgctt acgaaattgg cgcagaaaag atcatcgaca cctacgcgga
     3301 agcgactcgc catgtcgatc aggggctgtc gctgacgctg tttttccccg ataccgccac
     3361 cactcgcgat atcaacaaag cgcagattta cgcctggcgc aaaggtatca aaacgctcta
     3421 ttacatccgc ctgcgtcaga tggcgctgga aggcactgaa attgaaggct gcgtctcctg
     3481 tgcactttaa ggaatatcta tgaaactctc acgtatcagc gccatcaact ggaacaagat
     3541 atctgacgat aaagatctgg aggtgtggaa tcgcctgacc agcaatttct ggctaccaga
     3601 aaaggtgccg ctgtcgaacg atattcccgc ctggcagaca ttaactgtcg tagaacaaca
     3661 actgacgatg cgcgttttta ctggcctgac gctgctcgac acgctgcaaa atgttatcgg
     3721 cgcgccttcg ctgatgcccg atgcgctcac gcctcatgaa gaggcggtat tatcgaatat
     3781 cagctttatg gaagcggttc atgcccgctc ttacagttcg attttctcga cgctgtgcca
     3841 gaccaaagat gtcgatgccg cctacgcctg gagtgaagaa aacgcaccgt tgcagcgaaa
     3901 agctcagatt attcagcaac attatcgcgg tgatgatccg ctgaaaaaga aaatcgccag
     3961 tgtgtttctt gaatcttttt tgttctattc cggtttctgg ctgccgatgt atttttccag
     4021 ccgcggaaag ctgaccaata ccgcggacct gatccgtctg attatccgcg atgaagcagt
     4081 ccacggttac tacataggct ataaatatca gaaaaacatg gaaaagatat ctctgggaca
     4141 acgtgaagag ttgaagagtt tcgccttcga tttgttgctg gaactctacg acaacgagtt
     4201 gcaatacacc gatgagctgt acgccgaaac cccgtgggct gacgatgtga aagcgtttct
     4261 ctgttacaac accaataagg ctttgatgaa tctgggctac gaaccgttat ttcccgcaga
     4321 aatggcggaa gtgaatccgg caatcctcgc cgcgctttcg ccgaatgccg atgaaaatca
     4381 cgatttcttt tccggttcag gctcctctta tgtgatgggg aaagcggttg aaacagaaga
     4441 tgaagactgg aatttctgag ggtgttattt tcaaaaatat cactacccgc agcagggaaa
     4501 taattcccgc caaatagctt tttatcacgc aaatattctg tagtgatcta cactgatact
     4561 ctgttgcatt attcgcctga aactacaata ttcaggcgtt ttttcgctat ctatgacaaa
     4621 aaatatcaac ttttctcgat ttgctctcag cccttatatc acggaaaatt ccggcgattt
     4681 gctcgcatca atattcatgc cacatttgcc ctcaggggtt gcctcagatt ctcagtatgt
     4741 tagggtagaa aaaagtgact attcccattg ggtaatatat cgacataaac aaataaagga
     4801 atcattctat tgcatggcca ttaaattaga aattaaaaat ctttataaaa tatttggtga
     4861 gcatccacag cgagcgttca aatatatcga acaaggactt tcaaaagaac aaattctgga
     4921 aaaaactggg ctatcgcttg gcgtaaaaga cgccagtctg gccattgaag aaggcgagat
     4981 atttgtcatc atgggattat ccggctcggg taaatccaca atggtacgcc ttctcaatcg
     5041 cctgattgaa cccacccgcg ggcaagtgct gattgatggt gtggatattg ccaaaatatc
     5101 cgacgccgaa ctccgtgagg tgcgcagaaa aaagattgcg atggtcttcc agtcctttgc
     5161 cttaatgccg catatgaccg tgctggacaa tactgcgttc ggtatggaat tggccggaat
     5221 taatgccgaa gaacgccggg aaaaagccct tgatgcactg cgtcaggtcg ggctggaaaa
     5281 ttatgcccac agctacccgg atgaactctc tggcgggatg cgtcaacgtg tgggattagc
     5341 ccgcgcgtta gcgattaatc cggatatatt attaatggac gaagccttct cggcgctcga
     5401 tccattaatt cgcaccgaga tgcaggatga gctggtaaaa ttacaggcga aacatcagcg
     5461 caccattgtc tttatttccc acgatcttga tgaagccatg cgtattggcg accgaattgc
     5521 cattatgcaa aatggtgaag tggtacaggt cggcacaccg gatgaaattc tcaataatcc
     5581 ggcgaatgat tatgtccgta ccttcttccg tggcgttgat attagtcagg tgttcagtgc
     5641 gaaagatatt gcccgccgga caccgaatgg cttaattcgt aaaactcctg gcttcggccc
     5701 acgttcggca ctgaaattat tgcaggatga agatcgcgaa tatggctacg ttatcgaacg
     5761 cggtaataag tttgtcggcg cagtctccat cgattcgctt aaaaccgcgt taacgcagca
     5821 gcaaggtctt gatgcggcgc tgattgatgc gccgttagca gtcgatgcac aaacgcctct
     5881 tagcgagttg ctctctcatg tcggacaggc accctgtgcg gtgcccgtgg tcgacgagga
     5941 ccaacagtat gtcggcatca tttcgaaagg aatgctgctg cgcgctttag atcgtgaggg
     6001 ggtaaataat ggctgatcaa aataatccgt gggataccac gccagcggcg gacagtgccg
     6061 cgcaatccgc agacgcctgg gggacaccga cgactgcacc ggctgacggc ggtggtgctg
     6121 actggctgac cagtacgcct gcgccaaacg tcgagcattt taatattctc gatccgttcc
     6181 ataaaacgct gatcccgctc gacagttggg tcactgaagg gatcgactgg gttgttaccc
     6241 atttccgtcc cgtcttccag ggcgtgcgcg ttccggttga ttatatcctc aacggtttcc
     6301 agcaattgct gctgggtatg ccagcgccag tggcgattat cgttttcgct ctcatcgcct
     6361 ggcagatttc cggggtcgga atgggcgtgg cgacgctggt ctcgctgatt gccatcggcg
     6421 caatcggtgc ctgttcgcag gcaatggtga ctctggcgct ggtgttaact gccctgctgt
     6481 tctgtatcgt catcggtttg ccgttgggga tatggctggc gagaagtccg cgagcggcga
     6541 aaattattcg tccactgctt gatgccatgc agaccacgcc agcgtttgtt tatctggtgc
     6601 caatcgtcat gctatttggt atcggtaacg tgccgggcgt ggtggtgacg atcatctttg
     6661 cgctgccgcc gattatccgt ctgaccattc tggggattaa ccaggttccg gcggatctga
     6721 ttgaagcctc gcgctcattc ggtgccagcc cgcgccagat gctgttcaaa gttcagttac
     6781 cgctggcgat gccgaccatt atggcgggcg ttaaccagac gctgatgctg gccctttcta
     6841 tggtggtcat tgcctcgatg attgccgtcg gcgggctggg tcagatggta cttcgcggta
     6901 tcggtcgtct ggatatgggg cttgccaccg ttggcggcgt cgggattgtg atcctcgcca
     6961 ttatcctcga tcgcctgacg caggccgttg ggcgcgactc acgcagtcgc ggcaaccgtc
     7021 gctggtacac cactggccct gttggtctgc tgacccgccc attcattaag taactctgca
     7081 cttgcccggt gacgccgggc attatcaccc tgccaaaaaa aggaataaca atgcgacata
     7141 gcgtactttt tgcgacagcg tttgccacgc ttatctctac acaaactttt gctgccgatc
     7201 tgccgggcaa aggcattact gttaatccag ttcagagcac catcactgaa gaaaccttcc
     7261 agacgctgct ggtcagtcgt gcgctggaga aattaggtta taccgtcaac aaacccagcg
     7321 aagtggatta caacgttggc tacacctcgc ttgcttccgg cgatgcaacc ttcaccgccg
     7381 tgaactggac gccactccat gacaacatgt acgaagctgc cggtggcgat aagaaatttt
     7441 atcgtgaagg ggtatttgtt aacggcgcag cacagggtta cctgatcgat aagaaaaccg
     7501 ccgaccaata caaaatcacc aacatcgcac aactgaaaga tccgaagatc gccaaactgt
     7561 tcgataccaa cggcgacggt aaagcggatt taaccggttg taaccctggc tggggctgcg
     7621 aaggtgcgat caaccaccag cttgccgcgt atgaactgac caacaccgtg acgcataatc
     7681 aggggaacta cgcagcgatg atggccgaca ccatcagtcg ctacaaagag ggcaaaccgg
     7741 tgttttatta cacctggacg ccgtactggg tgagtaatga gctgaagcca gggaaagatg
     7801 tggtctggtt gcaggtgccg ttctccgcac tgccgggcga taaaaatgcc gataccaaac
     7861 tgccgaatgg cgcgaattac ggcttcccgg tcagcaccat gcatatcgtt gccaacaaag
     7921 cctggaccga gaaaaatccg gcggcagcca aactgtttgc cattatgcag ttgccagtgg
     7981 ccgatattaa cgcccagaac gccattatgc atgacggcaa agcctcagaa ggcgatattc
     8041 agggccacgt tgatggctgg atcaaagccc accagcagca gttcgatggc tgggtgaatg
     8101 aggcgctggc agcgcagaag taatttttat tcgggcggat aaggcgtttt cgcctcatcc
     8161 gccgctctgt acacaatgcc tgatgcgacg ctgacgcgtc ctatcatgcc tacaaaccac
     8221 atcgcacatt tcaacaagct atctttcatc ccatattcat caacatccgc tattattgat
     8281 ttccagctta atcatcacct gatgaacaaa aataatgact aaacctaatc atgagcttag
     8341 cccggcgctg atcgtgctga tgtctatcgc caccggtctg gcggtagcca gtaactatta
     8401 cgcccagcca ttgctcgaca ccatcgcgcg taacttttcc ctttccgcca gttcggcagg
     8461 ctttattgtt accgccgcgc agttgggcta tgccgcaggt ctactgtttc ttgttcccct
     8521 cggtgatatg tttgaacgcc gccgcctgat tgtctcgatg accttactgg cagcgggcgg
     8581 tatgttaatc actgccagca gtcagtcact ggcgatgatg atcctcggta cggcattaac
     8641 cggtttattc tcagtcgtgg cacaaattct ggttccgctg gcagcgacgc tggcttcacc
     8701 ggataaacgc ggcaaagtgg ttggcaccat tatgagcggt ctgctgttgg ggatattgct
     8761 ggcacgaacc gttgccgggt tactggcaaa cctcggcggc tggcgcaccg tcttttgggt
     8821 tgcttcggtg ttaatggcac tgatggcgct ggcattatgg cgtggtctgc cacaaatgaa
     8881 atcagaaacc cacctcaact acccacagtt gttgggttcc gttttcagca tgttcatcag
     8941 cgataagatc ctgcgcaccc gcgcgttgct gggctgcatg acctttgcca acttcagcat
     9001 tctctggacc tcaatggcct ttttgcttgc cgctccacct tttaactaca gcgatggtga
     9061 cattggtctg tttgggcttg cgggagctgc cggagcgttg ggcgctcgtc cggcgggcgg
     9121 ttttgccgac aagggcaaat cgcaccacaa ctttcggtct gcttctgctg ttactttcat
     9181 ggctggcaat ctggtttggt cacacttccg tactggcgtt gattatcggc attctggtgc
     9241 tggacctcac cgtacagggc gtgcatatca ctaaccagac ggtaatttat cgaatacatc
     9301 ctgatgcgcg taatcgcctg accgcaggtt acatgaccag ctactttatt ggcggtgccg
     9361 ccggttcgct aatttcagcc tcagcctggc aacatggcgg ttgggctggc gtttgtctgg
     9421 ctggcgcgac gattgccctg gttaacttac tggtctggtg gcgaggtttt catcgtcagg
     9481 aagccgcaaa ttaagcaatc agcaaccgtt ttcgtggctt tacacttata agggtgttaa
     9541 gaagcccatc agtctgataa ggttaagata ttcattcagt ctatttataa tattaacaat
     9601 cgttaagcgt acactctatg gaaagcccta ctccacagcc tgctcctggt tcggcgacct
     9661 tcatggaagg atgcaaagac agtttaccga ttgttattag ttatattccg gtggcctttg
     9721 cgttcggtct gaatgcgacc cgtctgggat tctctcctct cgaaagcgtt tttttctcct
     9781 gcatcattta tgcaggcgcg agccagttcg tcattaccgc gatgctggca gccgggagta
     9841 gtttgtgggt tgctgcactg accgtcatgg caatggatgt tcgccatgtt ttgtatggcc
     9901 cgtcactgcg tagccgtatt attcagcgtc tgcaaaaatc gaaaaccgcc ctatgggcgt
     9961 ttggcctgac ggatgaggtt tttgccgccg ccaccgcaaa actggtacgc aataatcgcc
    10021 gctggagcga gaactggatg atcggcattg ccttcagttc atggtcatcg tgggtatttg
    10081 gtacggtaat aggggcattc tccggcagcg gcttgctgca aggttatccc gccgttgaag
    10141 ctgcattagg ttttatgctt ccggcactct ttatgagttt cctgctcgcc tctttccagc
    10201 gcaaacaatc tctttgcgtt accgcagctt tagttggtgc ccttgcaggc gtaacgctat
    10261 tttctattcc cgtcgccatt ctggcaggca ttgtctgtgg ctgcctcact gcgttaatcc
    10321 aggcattctg gcaaggagcg cccgatgagc tatgaggttc tgctgcttgg gttactggtt
    10381 ggcgcggcga attattgctt ccgctatttg ccgctgcgcc tgcgtgtggg taatgcccgc
    10441 ccaaccaaac gtggcgcggt aggtattttg ctcgacacca ttggcatcgc ctcgatatgc
    10501 gctctgctgg ttgtctctac cgcaccagaa gtgatgcacg atacacgccg tttcgtgccc
    10561 acgctggtcg gcttcgcggt actgggtgcc agtttctata aaacacgcag cattatcatc
    10621 ccaacactgc ttagtgcgct ggcctatggg ctcgcctgga aagtgatggc gattatataa
    10681 cccacaagaa tcatttttct aaaacaatac atttacttta tttgtcactg tcgttactat
    10741 atcgactgaa attaatgagg tcatacccaa atggatagtt cgtttacgcc cattgaacaa
    10801 atgctaaaat ttcgcgccag ccgccacgaa gattttcctt atcaggagat ccttctgact
    10861 cgtctttgca tgcacatgca aagcaagctg ctggagaacc gcaataaaat gctgaaggct
    10921 caggggatta acgagacgtt gtttatggcg ttgattacgc tggagtctca ggaaaaccac
    10981 agtattcagc cttctgaatt aagttgtgct cttggatcat cccgtaccaa cgcgacgcgt
    11041 attgccgatg aactggaaaa acgcggttgg atcgaacgtc gtgaaagcga taacgatcgc
    11101 cgctgcctgc atctgcaatt aacggaaaaa ggtcacgagt ttttgcgcga ggttttacca
    11161 ccgcagcata actgcctgca tcaactctgg tccgcgctca gcacaacaga aaaagatcag
    11221 ctcgagcaaa tcacccgcaa attgctctcc cgtctcgacc agatggaaca agacggtgtg
    11281 gttctcgaag cgatgaacta acgcgtcatc tcgctcaaaa atccagattt ataaaagaaa
    11341 aaatgaccgg ccagcatcgc aacatgctgg cctttttggc aagcaggtcg gctcagccga
    11401 tgagttaaga agatcgtgga gaacaatatg agcgcaaatg cggagactca aaccccgcag
    11461 caaccggtaa agaagagcgg caaacgtaag cgtctgctcc tccttctcac cttgctcttt
    11521 ataattattg ccgtagcgat agggatttat tggtttttgg tactgcgtca tttcgaagaa
    11581 accgatgacg catacgtggc agggaatcag attcaaatta tgtctcaggt gtctggcagc
    11641 gtgacgaaag tctgggccga taacaccgat tttgtaaaag aaggcgatgt tctggtcact
    11701 ctcgacccga cagatgctcg ccaggcgttt gaaaaagcca aaactgcact ggcttccagc
    11761 gttcgccaaa cccaccagct gatgattaac agcaaacagt tgcaggcgaa tattgaggtg
    11821 caaaaaatcg ccctcgcgaa agcacaaagc gactacaacc gccgtgtgcc gctgggcaat
    11881 gccaacctga ttggtcgcga agagctgcaa cacgcccgcg acgccgtcac cagtgcccag
    11941 gcgcaactgg acgtcgctat tcaacaatac aatgccaatc aggcgatgat tctggggact
    12001 aaactggaag atcagcctgc cgtgcaacag gctgccaccg aagtacgtaa cgcctagctg
    12061 gcgctggagc gtacccgtat tgtcagtccg atgaccggtt atgtttcccg tcgtgccgtg
    12121 caaccgggtg cgcaaattag cccaacgacg ccgctgatgg cggtcgttcc agccaccaat
    12181 atgtgggtgg atgccaactt taaagagacg cagattgcca atatgcgcat cggtcagccg
    12241 gtcaccatca ccaccgatat ttacggcgat gatgtgaaat acaccggtaa agtggttggt
    12301 ctggatatgg gcacaggtag cgcgttctca ctgcttccgg cgcaaaatgc gaccggtaac
    12361 tggatcaaag tcgttcagcg tctacctgtg cgtatcgaac tggaccagaa acagctggag
    12421 caatacccgc tgcgcattgg tttgtccacg ctggtaagcg tcaataccac taaccgtgac
    12481 ggtcaggtac tggcaaataa agtacgttca acaccggtcg cggtaagtac cgcgcgtgaa
    12541 atcagcctag cacctgtcaa taaactgatc gacgatatcg taaaagctaa cgctggctaa
    12601 tccagaggtg cgtgtgatgc aacagcaaaa accgctggaa ggcgcgcaac tggtcattat
    12661 gacgattgcg ctgtcactgg cgacattcat gcaggtgctg gactccacca ttgctaacgt
    12721 ggcgatcccc actatcgccg ggaatctggg ctcatcgctc agccaaggaa cgtgggtaat
    12781 cacttctttc ggggtggcga atgccatctc gatcccgctt accggctggc tggcaaagcg
    12841 cgtcggggaa gtgaaactgt tcctttggtc caccatcgcc tttgctattg cgtcgtgggc
    12901 gtgtggtgtc tccagcagcc tgaatatgct gatcttcttc cgcgtgattc aggggattgt
    12961 cgccgggccg ttgatcccgc tttcgcaaag tctattgctg aataactacc cgccagccaa
    13021 acgctcgatc gcgctggcgt tgtggtcgat gacggtgatt gtcgcgccaa tttgcggccc
    13081 gatcctcggc ggttatatca gcgataatta ccactggggc tggatattct tcatcaacgt
    13141 gccgattggc gtggcggtgg tgttgatgac actgcaaact ctgcgcggac gtgaaacccg
    13201 caccgaacgg cggcggattg atgccgtggg gctggcactg ctggttattg gtatcggcag
    13261 cctgcagatt atgctcgacc gcggtaaaga gctggactgt ttttcatcac aggaaattat
    13321 cattcttacc gtggtggcgg tggtggctat ctgcttcctg attgtctggg agctgaccga
    13381 cgataacccg atagtcgatc tgtcgttgtt taagtcgcgc aacttcacca tcggctgctt
    13441 gtgtatcagc ctcgcgtata tgctctactt cggcgctatt gttctgctgc cgcagttgtt
    13501 gcaggaggtc tacggttaca cggcgacctg ggcaggtttg gcctctgcgc cggtagggat
    13561 tattccggtg attctgtcgc cgattatcgg ccgcttcgcg cataaactgg atatgcggcg
    13621 gctggtaacc ttcagcttta ttatgtatgc cgtctgcttc tactggcgtg cctatacctt
    13681 tgaaccaggt atggattttg gcgcgtcggc ctggccgcag tttatccagg ggtttgcggt
    13741 ggcctgcttc tttatgccgc tgaccaccat tacgctgtct ggtttgccac cggaacgact
    13801 ggcggcggca tcgagcctct ctaactttac gcgaacgctg gcggggtcta tcggcacgtc
    13861 gataaccacg accatgtgga ccaaccgcga gtcgatgcac catgcgcagt tgactgagtc
    13921 ggtaaacccg ttcaacccga atgcccaggc gatgtacagt caactggaag ggcttgggat
    13981 gacgcaacag caggcatcag gctggattgc ccagcagatc accaatcagg ggctgattat
    14041 ttccgccaat gagatcttct ggatgtcagc cgggatattc ctcgtactgc tggggctggt
    14101 gtggtttgct aaaccgccat ttggcgcagg tggcggcgga ggcggtgcgc actaagtaca
    14161 actaagccag ttcatttgaa ctggcttttt tcaattaatt gtgaagatag tttactgact
    14221 agatgtgcag ttcctgcaac ttctctttcg gcagtgccag ttcttcgttg ctgttgatgc
    14281 gtacgtcacg ttccagaatg ctacgcgcaa tatcctgcgc ttcctgcaac gagtgcatct
    14341 ggtaagtgcc acactggtag acgttcagtt ccgggatctg attctgatcc tgcactttca
    14401 gcacgtcttc cattgccgct ttccaggcat cagcaacacg ctgctcatct ggcgtaccaa
    14461 tcagactcat ataaaaaccg gtgcggcagc ccattggcga gatatcgata atctctacac
    14521 cattaccgtt aagatggtta cgcataaaac cagcaaacag gtgctccagg gtatggatcc
    14581 ctctttctgg catcacttct ttgttcggca cgcagaagcg cagatcgaac acggtgattg
    14641 cgtcgccatg cggggtgttc attgttttcg ccacccgaac tgcaggcgct tccatccggg
    14701 tatgatcgac tgtgaagcta tctaacaacg gcatttagcc acctccggta atttttttaa
    14761 aaattttctg aactctttct tcccaggcga gtctgagtat atgaaagacg cgcatttgtt
    14821 atcatcatcc ctgaattcag agatgaaatt ttggccactc acgagtggcc tttttctttt
    14881 ctgtcaggcg tgtttttcca gccacaccgc aaacggttcg gtatcagcgg tttccatttc
    14941 ctgctgacgg cgttcagatg cctcgcgctc ggctacaaaa tcctcttcgc gcagaatttc
    15001 cagcggctct tcacgcagca gattacggta ggcttctgca aacgctttgc ctgttccgcc
    15061 aataccagta tcaatcatag accttaagat acgggcagag aaagtcagat cgggattatc
    15121 gaagcaggca accagttcat cacacacttt ctgatacgct tcgccgccgt taatgctatc
    15181 cagcgtttgc gcgacgcgtt tcagatcgtg gaacagatct ttacccacct gcagtaacgg
    15241 gaactgcgcg gtttcgcagc cgatacccag cgtcaggccc ggtttgcgac cttcaaggat
    15301 cacccggttc cagttaacgc gcgtacaggc aagttcgcta ctgctcattt ccggtgcatc
    15361 agccagcgca caccagacca taaacaggtc gaggaatcgc acctgctgtt catctacacc
    15421 aatcggcgag aacgggttga tgtccagcga acgcacttca atatattcaa tgccgccacg
    15481 taacagcgca tcagaaggtg actcgccgct gcgggtaacg cgttttggac gaatcggcgc
    15541 atacagttcg ttttcaatct gcagcacgtt gctattgatt tgcagcctct taccgtcttt
    15601 ctcaatacca atcttcgcgt actcttccga tggcgttttg attgcctgtt taaggcccgc
    15661 tacgtactcg taaagatcgt tgaaggtaat accaagattg ctttgcgatt tattggtata
    15721 gccgagatcg ctcaaacgaa gagaggtcgc atacggcagg taatacatac cgcactcggt
    15781 tttctcaaac ggcagcgacg ttggttttcc ttgcaggaaa gaagaacaaa tcgccggaga
    15841 tgcaccaaac agataaggaa tgacccaacc gaaacgatag taattgcgga taacgcggaa
    15901 atagcccgca gaaattttct ctttggcatc agcgcccgag atatcaccgc acttcgcttg
    15961 ccagaatgcc attggcaaag agaaattgta gtgcacgccg gaaatggttt gcatcagcgc
    16021 gccgtagcga tttttcagcc cttcacgata cagcgtttta aagcgtccgg tgttagaagt
    16081 gccgtactgt gccagttcga tgtcctgacc ttctgcgatg tagcatggca tacttaacgg
    16141 ccacatccgc tcatcgccca tattgcgcgc cgtataacga tgcagatcgc gcataaaggt
    16201 cagcatatgt tcaatatcac catccactgg tgtaatgaat tccagcaatg cttccgcaaa
    16261 atcggtagta atccatttgt gcgtcagtgc ggaacctaat gcttcaggat gacctgttgt
    16321 tgccagtgtg ccatcagcat taacacgcaa agtttcgcgc tccagcccac gctgtatccc
    16381 ctttaacgcc tgaggatgtt tttccagcca ggccagcgcc tgtgatacgt ccgggatcaa
    16441 attgacctcc cgcctgtcaa aatcgtttta attagcataa ctgtaatggt gaccatatgt
    16501 gcaggcctac aattagtgcc accacatcat gccctgaacg gtcgctgctg caactgcaac
    16561 atagcgtaac gctttaccaa ggcacaaaaa aaagattact ggcccccagg agatgcgcat
    16621 ccatcccgct aacagacaca gtaaatcgcc aaccacgggc atccagctta ataatagcgt
    16681 gactgcacca tagcgtttca gccagccggt agctttctct tgccagcgcg atgttttacg
    16741 caatggaaag aaacgcccaa ggataacgtt agttaaccct ccaaggctat tacccattgt
    16801 tgctgttaaa actaaaaccc agggatgact gatcccggaa agcaacattg ccaccagcac
    16861 gacttcggag ttgccgggta atagtgtagc gctgagaaaa ctactggcaa acaacgagaa
    16921 aagcgataac gcttcactca cagcaagcga acatccacag cgtccatgcc tgctgcacgg
    16981 gccgcctgaa taccgaaatc ggcatcttca aagaccacac actgcgtcgg gtgcacgccc
    17041 atacgctgcg cgcacaacaa aaatgtgtct ggcgcgggtt tatggtgttt gacgtgatca
    17101 gcagcgacga cggcgtcaaa ataacggcgt aatcccaggt gcgccagcaa tgcctctgcg
    17161 atggcgcttt cactccccgt tcctacagcc attggacgac gaccatgcca acttttcacc
    17221 acttcaacaa gaggaagcgg ctcgacacta tccagcagca tacttcttac tgcttctgtt
    17281 ttttcacgcg ctaacgcatg cgggtcgaga tcggcctgat tcagctcaat aattgcctga
    17341 gcaatacgcc aggtgggcga tccattaagc gcaatcatcg cctgaatatc gtactgaaga
    17401 ccgtagtgcc ctaatacttc gcgccacgct ttacggtgcg taggctccgt atccaggatt
    17461 gtgccatcca tatcaaaaat taaacctgca taacgctcgt acatggtctt ctcgcaagtc
    17521 gaaaaatatg acgctacttt agcgtaattg cttgattttg tcgctgatgg cgaaggagaa
    17581 gaaaattcag gaaatggata aagtagttat ttagaaatga gatatttttg aaggaaattt
    17641 tttggagaat atggtgcatc cgggaggatt cgaacctccg accgctcggt tcgtagccga
    17701 gtactctatc cagctgagct acggatgcat cgggaaactt actttactgc aggttttttg
    17761 ataccgctac taaagccgta tcaagtaaga gatggtgcat ccgggaggat tcgaacctcc
    17821 gaccgctcgg ttcgtagccg agtactctat ccagctgagc tacggatgca tcgggaaact
    17881 tactttactg cagatttttt gataccgcta ctaaagccgt atcaagtaag agatggtgca
    17941 tccgggagga ttcgaacctc cgaccgctcg gttcgtagcc gagtactcta tccagctgag
    18001 ctacggatgc aaatggcggt gaggcgggga ttcgaacccc ggatgcagct tttgaccgca
    18061 tactccctta gcaggggagc gccttcagcc tctcggccac ctcaccacac gcctcttacg
    18121 agtgcttcga agaacttgtt tatcgctcat cgtcgctgcg tggcgcacat attactttct
    18181 gagacttata agtcaaacaa tttttcccac acttttatcg tttgcacact tcacgctcaa
    18241 ttagtctgta aaaacgtcaa aaagagtgtt ttatctacag aaaaacggag gtctggcaga
    18301 tagcagtaat gcaaaaaaat ggagacttaa gttgaatgaa cgggagtaaa gcgaaaagac
    18361 tatagagtga aggagaaatt ttgagggtgc gtctcaccga taaagatgag acgcggaaag
    18421 attagtaact ggactgctgg gatttttcag cctggatacg ctggtagatc tcttcacggt
    18481 gaacagaaac ttccttcggg gcatttacgc caatacgtac ctggttgccc tttaccccta
    18541 aaactgtcac ggtgacctca tccccaatca tgagggtctc accaactcga cgagtcagaa
    18601 tcagcattct ttgctccttg aaagattaaa agagtcgggt ctctctgtat cccggcatta
    18661 tccatcatat aacgccaaaa agtaagcaac gacaaacaca ttacatgtaa gcagtcatgg
    18721 cattacattc tgttaaacct aagtttagcc gatatacaca acttcaacct gactttatcg
    18781 ttgtcgatag cgttaatgcg aatgccgtga agcgagtcca cggcattgcc tgacgcttat
    18841 attattgcaa tttcgcgctg acccagcctt tcacactggc taacgctgca ggtaaggccg
    18901 cagcatccgt accaccggct tgcgccatgt caggacgtcc accacccttg ccgcccacct
    18961 gctgagcgac cataccaatc agttcccctg ctttcacacg atctgtgacg tccttagata
    19021 cgcctgcaat cagagaaacc ttaccttcgg ctaccgttgc cagcacgata attgtcgacc
    19081 ccagctgatt ttttaaatcg tcaaccatgg tacgcaacat tttcggctca acaccgctaa
    19141 gctcgctaac caacagctta acaccattaa catcaattgc cttactggaa agatttgcgc
    19201 tctcctgtgc ggcagcttgt tctttaagct gttgtagttc tttttctaac tgacgcgtac
    19261 gttccagtac tgagcgcact ttatccgcca gattattgct atcgcctttc agcagatgcg
    19321 cgacttcgct taagcgatcg ctgtctgcat gaacggtggt gatagcgcct tctccggtta
    19381 ccgcttcgat acgacgaacg cctgcagcag tacccgattc agagatgatg cggaacagac
    19441 caatatcacc agtgcggctg gcgtgagtac cgccacacag ctcggtggag aaatcgccca
    19501 tgctcagcac gcgtacacgc tcatcatact tctcgccgaa cagcgccatc gcacctttcg
    19561 ctttcgccgc ttcgagattc atgatgttgg tttcgatcgg caaattgcga cgaatctgtg
    19621 cgttcaccag gtcttcgacc gcacgaatct cttccggttt catcgcttcg ttgtgtgaga
    19681 agtcgaagcg cagcaccttg tcgttaacca gtgaaccttt ctgcgataca tgagtcccca
    19741 gaacctggcg cagcgcagcg tgcatcaggt gcgttgcgga gtgattcaga cgaatacggg
    19801 cgcgacgagc ctcatcaata tcagcctgca cagcgtcgcc cactttcaga gaacccgcag
    19861 caagtttacc gatgtgacca atcgcctggc cgtatttctg cgtatcttcc accgcgaagg
    19921 agaagttagc gcctttcagt tcgcctttat caccaacctg accgccggat tccgcataga
    19981 atggcgtttg atccagcacg accacagctt cctggcctgc attgatggca tcaaccgctt
    20041 taccatcaac aaacagcgcg gtcactttgc cgttcagttc cagatggtca tagcctttaa
    20101 attcagatgc actatcaaca cggatcattg cgttgtaatc ggcaccaaag ccgctggctt
    20161 cgcgcgcgcg acgacgctgc tcttccattg ctgcttcaaa accagcttcg tcaactttga
    20221 tgttgcgctc acgacaaaca tcagccgtca ggtcaaccgg gaagccatag gtgtcgtaca
    20281 gacggaaagc cgtttcacca tccagcgtat caccagaaag ttttgccagc tcttcatcca
    20341 gcaacgccag accgcgctcc agagtacggg caaactgctc ttcttcagtc ttcagcacct
    20401 gctcaacctg cgcctgctgg cgtttcaggt cttcacccgc agagcccata acgtcgatca
    20461 gcggaccaac cagtttgtag aagaaggttt ctttcgcgcc gagcatattg ccgtgacgca
    20521 ctgcgcgacg aatgatacga cgcagtacat aaccacggtt ttcattggac ggcattacgc
    20581 catccgcgat caggaacgca caagaacgaa tgtggtcagc gattacgcgc agcgatttat
    20641 tgctcagatc ggtcgcgcca gtgactttcg ctaccgcctg gatcaacgtg cggaacaggt
    20701 cgatgtcata gttagagtta acgtgttgca gcaccgcagc aatacgctcc agacccatac
    20761 cggtatctac agacggcttc ggcagcggtt ccatcgtgcc atctgcctgg cggttgaact
    20821 gcatgaagac gatgttccag atctcaatgt agcggtcgcc gtcttcttcc ggacttcccg
    20881 gagggccacc ccaaatgtgg tcgccgtgat cgtagaagat ttcggtgcac gggccgcacg
    20941 gaccagtgtc acccatctgc cagaagttgt cagatgcgta tggcgcacct ttgttatcgc
    21001 cgatgcgaat aatacgttcg cgcgggatcc cgacttcttt ttcccagatt tcgtaggctt
    21061 cgtcgtcgct ttcatagacg gtaacccaca gacgctcttt cggcagggca aaccattttt
    21121 cgctggtcag cagttcccat gcaaactgaa tggcatcgtg tttgaaatag tcgccgaagc
    21181 tgaagttgcc cagcatttcg aagaaggtat ggtgacgcgc ggtgtaaccg acgttttcca
    21241 ggtcgttgtg tttaccaccc gcacgcacgc agcgttggga agtggtagcg cgggaataat
    21301 tacgcttgtc gagcccaagg aacacatcct tgaactggtt catcccggcg ttggtaaaca
    21361 acaaagttgg gtcgttatgg ggtaccaggg agctgctggc aactacctga tgtcccttac
    21421 tatggaaaaa gtcgagaaac gcctgacgga tctcagcggt gctcttgctc ataattatcc
    21481 tgaaatcaag ctaacgaaat atcgccacca gctccagcgt gtcttaaccg ccgggctggt
    21541 aactgaaaag tgggaataag ataagttttc ttgactggga agtaaaatac cgtatgcgtt
    21601 cagtcggcaa aatttcgcca aatatcctgg atatcttcca tcagatagcc acgatagagc
    21661 agaaaacgct ggatcttaac tttttctgaa aagacagttg gcaaaggttc accatatttt
    21721 cgcgtcgcct gatcgcgcgc cagtgcgcac cagtcgatgt cacattcacg catcgctttt
    21781 tctgtcgctt cgcgggaaat acctttctga ttcagttcct gacgaatacg tgcaggtcca
    21841 taacctttgc ggctacggct ggcgataaag cgcgcaacaa atcggctgtc atcgagatag
    21901 ccatgttcat ggcaccaggc aataacgcgc tcgtaatctt ctgccgtagc atcaatctct
    21961 tctgggccat ttttgcccat aatcggtgcc gcgagtttac gtcgcagttc ttgctcactg
    22021 tgatcgcgca ccgccagaat gcgtaccgca cgatccaaca ggcgagcata tgccgggcga
    22081 cgggatgttg attctgtcat ggcatatcct tacaacttaa aaaagcaaaa gggccgcaga
    22141 tgcgacccag gttttgctga taaacagatt taatagtcaa tgagtatgca gaatgttccc
    22201 gcaggcccaa ttcgttagag gatcgaggtg agagtaattt ttcacgccag tgctggcctg
    22261 ttcccctcac cctaaccctc tccccaaagg ggcgagggga ccgtccgtgc acacattatc
    22321 tattatcatc aataacgaac gattaaaaat cttcgttagt ttctgctacg ccttcgctgt
    22381 catctacaga gaaatccggc gttgagttcg ggttgctcag cagcaactca cgtactttct
    22441 tctcgatctc tttcgcggtt tccgggttat ctttcagcca ggcagttgca ttcgctttac
    22501 cctgaccgat cttctcacct ttgtagctgt accacgcgcc tgctttctcg atcagcttct
    22561 cttttacgcc caggtcaacc agttcgccgt agaagttgat accttcgccg tagaggatct
    22621 gaaattcagc ctgtttaaac ggtgcagcga ttttgttctt caccactttc acgcgggttt
    22681 cgctacccac cacgttttcg ccctctttca ccgcgccgat acgacggatg tcgagacgaa
    22741 cagaggcgta gaatttcagc gcgttaccac cggtagtggt ttccgggtta ccgaacatca
    22801 caccaatttt catacggatc tggttgatga agatcagcag cgtgttggac tgcttcaggt
    22861 tacccgccag cttacgcatc gcctggctca tcatacgtgc cgcaaggccc atgtgagagt
    22921 cgccgatttc gccttcgatt tccgctttcg gcgtcagtgc cgccacggag tcaacgacga
    22981 taacgtctac tgcgccagaa cgcgccaggg cgtcacagat ttccagtgcc tgctcgccgg
    23041 tatccggctg ggagcacagc aggttatcga tatcgacgcc cagtttacgt gcgtagattg
    23101 ggtccagcgc gtgttcagca tcgataaacg cacaggtttt accttcacgc tgcgctgcgg
    23161 cgatcacctg cagcgtcagc gtggttttac cggaagattc cggtccgtag atttcgacga
    23221 tacggcccat cggcagacca cctgccccaa gcgcgatatc cagtgaaagc gaaccggtag
    23281 agatggtttc cacatccatg gaacggtctt cacccaggcg catgatggag cctttaccaa
    23341 attgtttctc aatctggccc agtgctgccg ccaacgcttt ctgtttgttt tcgtcgatag
    23401 ccatttttac tcctgtcatg ccgggtaata ccggatagtc aatatgttct gttgaaacaa
    23461 ttatactgta tgctcataca gtatcaagtg ttttgtagaa attgttgcca caaggtctgc
    23521 aatacatacg cagtagcctg acgacgcacc gcatcacggt cgccgctgaa gcattcccgc
    23581 cgggtaatgc cttcaccgcg ggcagtggca aaagcaaacc agacggtgcc gacaggcttc
    23641 tcttcactgc cgccatccgg cccggcgata ccactaatag acacggcata atcagcacga
    23701 gccgctttca gtgcacctat cgccatttcc accacgacgg gttcactcac cgcgccatgc
    23761 tgcgccagcg tctcttcgcg tacgccgatc atctgcgctt tggcttcgtt actgtaggtg
    23821 acaaatccgc gttcaaacca ggcggagcta ccggcaatat cggtaatcac tttcgctacc
    23881 caaccaccgg tacaagactc ggcagttgtt acggttgcgc cacgggcttt cagcgcctgc
    23941 ccaacctgtt cgcttaactg catcagttca ctgtcagtca tcacaacctc tgtcagtcaa
    24001 aaatttatgc cggacaagat agcactttcg tcaggaagat ggtgttggcg ttgcaatttt
    24061 acgaggagga ttcagaaaaa agctgattag ccagagggaa gctcacgccc ccctcttgta
    24121 aatagctact gtactcgcgc cagcgccacg gcttgtccta actgccagac cgccattgcg
    24181 taatgggtgc tgtggttgta acgggtgatg gtgtagaagt tcggcagacc gtaccagtac
    24241 tggtagccgg tgccaacatc cagacgcagc aggctggctt gttgatggtt gcccagcggc
    24301 tgctgtggcg ttaaacctgc ggcggcaagc tgcgaaatgc tgtacttagt tttgaaacca
    24361 tttggcaagc ctggagcctg accgtttgcc attatcgcga cctgatcgcc tttcacccag
    24421 ccgtgcgctt tgaaatagtt cgccacgcta ccgatcgcat caaccggatc ccacaagttg
    24481 atatgcccgt cgccgctgaa atctaccgca tattgtttgt aagacgacgg cataaactgt
    24541 ccgtagccca tcgccccggc aaaggaacct ttcagattga gcggatcgtc ctgctcgtcg
    24601 cgcgccatca gcaggaaggt ttccagctcg ccagagaaat actccgcgcg gcgtgggtag
    24661 ttaaatgaca gcgttgccag cgcatcgagg atgcgagttt tccccatcac gcgcccccag
    24721 cgggtttcaa cgccgataat cccgacgata atttccggcg gtacgccata cacctgccac
    24781 gcgcgattca acgcatcttc atactgattc cagaaaacca caccgttctg cacgttgtcc
    24841 ggcgtaataa attttttgcg ataacgcagc catgcgccgt tcggacctga tggcggtttc
    24901 accgatgtgg ttggtgcctg gttatccatc agccgcagta ccgaatccag acgcttcgcc
    24961 tgggagagaa tttcctgcaa ctgctgacga tcgaaaccgt gtttgttcac cattttgtcg
    25021 atgaactgct gggcattcgg gttattagcg aaatcgccgc ccatctgcat cacattgtgc
    25081 tgcggctcaa gcaggaagcc gccagacggc gttccggtgg tcgtttcagt ctcagtaggt
    25141 tttggcttgc tgctacaggc ggcaagcaac acaaaaaggg gaagcaatgt tacataacga
    25201 cgcttgaaca tgaggggtcc atttaacaga ttcaaccagg ggcaagtatg gtaaagcatc
    25261 acgccccgca caaggaagcg gtagtcactg cccgatacgg actttacata actcaactca
    25321 ttcccctcgc tatactttta ttcaaacttt caaattaaaa tatttatctt tcattttgcg
    25381 atcaaaataa cacttttaaa cctttcaacc tgattagatt aggttgccgt ttggtaataa
    25441 aacaataaat cctgaaggag agaacaatga tagaaaccat tactcatggt gcagagtggt
    25501 ttatcgggct gttccaaaag ggcggagagg tgtttaccgg aatggtgacc ggcattcttc
    25561 cgctgttgat tagcctgctg gttatcatga acgcactgat taattttatc ggtcagcatc
    25621 gtattgaacg ttttgctcaa cgttgcgccg gtaaccctgt ttcccgttac ctactgttac
    25681 cgtgcattgg cacgtttgtc ttttgcaatc cgatgacctt aagcctaggt cgctttatgc
    25741 cggaaaagta caaacccagc tactacgcgg cggcctctta tagctgccac tcaatgaatg
    25801 gcctcttccc ccatatcaac cctggcgaac tgtttgttta tcttggcatt gccagcggtc
    25861 tgacaacgct gaacctgcca cttggcccac tggcggtgag ttatctgctg gttggtctgg
    25921 tcaccaattt cttccgcggc tgggtgaccg atctgaccac cgccattttt gagaaaaaga
    25981 tgggcattca acttgaacaa aaagttcacc tggcaggagc aacatcatga cgcgtattcg
    26041 gatcgaaaaa ggaacgggtg gctggggcgg cccgcttgag ctggaagcta cgccgggcaa
    26101 aaaaatcgtc tatatcaccg ccggtacccg gcctgcgatt gttgacaaac tggcacaact
    26161 tactggctgg caggctattg acggatttaa agaaggtgaa cccgcggagg cggaaattgg
    26221 tgtcgcggta atcgactgtg gcggcacatt acgctgcggc atctatccga aacgacgtat
    26281 tcccaccatt aatatccact cgacgggcaa gtccggtccg ctggcgcagt acattgtgga
    26341 agatatttat gtctctggcg taaaagaaga aaacatcact gtagtgggtg atgcgacacc
    26401 acaaccctct tccgtgggcc gtgactatga caccagtaag aaaatcaccg aacaaagcga
    26461 tggtttactg gcgaaggtgg gaatgggcat ggggtccgcc gttgccgtgt tgtttcagtc
    26521 tggtcgtgac accatcgaca ctgtattaaa aaccattctg ccgtttatgg cattcgtctc
    26581 ggcgctcatt ggcatcatta tggcttctgg ccttggtgac tggattgccc acggtcttgc
    26641 tccgctggcg agccatccac tgggtctggt catgctggcg ctcatctgct ccttcccact
    26701 gctttcacct ttcctcggcc caggcgcagt tatcgcacag gttatcggcg tattgattgg
    26761 cgtgcagatt ggtctcggca atattccgcc gcatctggct ttaccggcac tgtttgccat
    26821 caacgcgcag gcggcctgcg acttcatccc ggtcggtttg tcgctggcgg aagcccgtca
    26881 ggacacggtt cgcgtcggtg tcccttctgt actggtgagc cgctttttaa ccggcgcgcc
    26941 gactgtactg atcgcctggt ttgtctccgg ttttatctat caatagaggc tgaaacatga
    27001 ccgttattta tcagaccacc atcacccgta tcggcgcgag tgccactgac gccctcagcg
    27061 accagatgct catcaccttt cgtgaaggcg cgccagcgga cctcgaagag tattgcttca
    27121 ttcattgcca cggcgagttg aaaggtgcac tccatcccgg tttgcaattt tcactcgggc
    27181 agcatcgcta tccggtgacc gctgttggca gcgtggcgga agacaacctt cgcgaactgg
    27241 gtcatgtcac cctgcgcttc gatggtttaa acgaagcgga atttccgggc actgtccatg
    27301 tggcaggccc tgtacccgac gatatcgcgc cgggatcggt tttgaagttt gaatctgtta
    27361 aggagtaaaa aatgaatcag gttgccgttg tcatcggtgg tgggcaaacc ttaggcgcgt
    27421 tcctgtgcca cggtctggct gccgaggggt atcgcgtcgc ggttgtcgat attcagagcg
    27481 acaaagccgc aaatgtggca caagaaatta acgccgaata tggtgaaggt atggcgtacg
    27541 gttttggtgc tgacgccact agcgagcaaa gcgttctggc gctctctcgt ggggtagatg
    27601 aaatctttgg tcgcgtggat ttgctggtct atagcgccgg aatagcaaaa gcagccttta
    27661 tcagcgactt ccagctcggc gattttgacc gttcgctaca agtgaatctg gtgggttatt
    27721 tcctgtgtgc ccgtgaattt tcgcgtttga tgatccgcga cgggattcag ggtcgcatta
    27781 ttcagatcaa ctcgaaatcc ggcaaagtgg gcagcaaaca caactctggc tacagcgcag
    27841 cgaaatttgg tggcgtcggg ctgactcaat cactggcgct ggatctggcg gagtacggca
    27901 ttacggtgca ttcactgatg ctcggtaacc tgctgaaatc gccgatgttc cagtcactgt
    27961 tgccacaata cgcgaccaag ctgggtatca aaccggatca agtcgagcag tattacatcg
    28021 acaaagtacc gctcaaacgc ggctgcgatt atcaagatgt gctgaatatg ctgctgttct
    28081 acgccagtcc taaggcgtcg tactgcaccg gacagtcgat caatgtcacc ggcggtcagg
    28141 tgatgttctg atcaacagcg gagatccgtt aaggatctcc gtgagactat agaatgcctg
    28201 atgcgctacg ctcatcaggc atacaggact tccgccacta cattaaggaa aagttatggt
    28261 atccgcactc atcaccgtcg ccgttatcgc ctggtgtgcg caactggcct taggcggctg
    28321 gcaaatttct cgttttaacc gtgccttcga cacactatgc cagcaaggga gggttggcgt
    28381 gggccgttcc agcgggcact ttaaaccgcg ggtcgtggtc gccatcgcgc tggacgatca
    28441 gcagtgcatc gtcgacatct tgtttatgaa aggactgact gtcttcgccc gaccgcaaaa
    28501 aattcccgca attaccggca tgcatgcggg tgatttacag cccgatgtga tctttcccca
    28561 tgatccacta tcacagaatg ctctatcatt ggcgcttaaa ctgaaacgtg gataatttcg
    28621 ttgtgaatgt tacttgcttg cgaagttatc attttgaaac ctaaatcagg taatcacgcc
    28681 catgaaacct cgtcagcgtc aggccgccat tctggagtat ctgcaaaagc agggtaaatg
    28741 ctcggttgaa gaattggcgc aatactttga caccacaggc acaaccattc gcaaagatct
    28801 ggtcattctg gaacatgccg gaaccgtcat tcgtacttat ggcggagtgg tgttgaataa
    28861 agaggaatcc gatccgccta tcgatcataa aacgctcatc aacacccaca agaaagagct
    28921 gattgcagaa gctgccgtta gttttatcca tgatggcgat tcgatcattc ttgatgctgg
    28981 cagtaccgtt ttgcagatgg ttcccctgct ctcgcgcttt aataacatca cggtgatgac
    29041 caacagcctg catatcgtca atgcgctatc cgaactggat aacgaacaaa ctatcctgat
    29101 gccaggcgga acatttcgca aaaaatcggc ctcatttcac gggcaactgg cagagaatgc
    29161 cttcgagcat ttcaccttcg ataaattgtt tatgggcacc gacggcatcg atctcaatgc
    29221 gggcgtaacc acgttcaacg aggtttatac cgtcagtaag gcaatgtgca atgccgcgcg
    29281 cgaagtgatt ttgatggcgg actcatcaaa gtttggccgt aaaagcccca acgtagtttg
    29341 cagtcttgaa agcgtcgata agctaattac cgacgcaggt atcgatccgg cgtttcgtca
    29401 ggcgctggaa gagaaaggga tcgatgtgat cataaccgga gagagcaatg agtgaagcac
    29461 tactgaacgc gggacgtcag acgttaatgc tggagttgca ggaagcaagc cgtttaccgg
    29521 aacgtctggg cgatgatttt gttcgcgccg ccaatatcat cctgcactgc gaaggcaaag
    29581 tggtggtttc gggaattggc aaatcgggcc acattggtaa gaaaatcgcc gcaacacttg
    29641 ccagtaccgg cacaccggct ttttttgtcc atccggcaga agcactgcac ggcgatctgg
    29701 ggatgattga aagccgcgat gtgatgctgt ttatctctta ctccggtggc gcgaaggaac
    29761 tggatctgat tattccgcgt ctggaagata aatctatcgc gctgctggcg atgaccggca
    29821 aaccgacgtc accgctgggc ctggcggcga aagcggtgct ggatatctcc gtagaacgcg
    29881 aagcctgccc gatgcacctt gcgccgacct ccagcaccgt caataccctg atgatgggtg
    29941 acgcgctggc gatggcggtc atgcaggcgc gcggttttaa tgaagaagat tttgcccgct
    30001 cccacccagc tggggcactg ggcgctcgct tgctgaataa agtgcatcat ctgatgcgtc
    30061 gtgatgacgc tatcccacag gtagcgttaa ccgccagcgt gatggatgcg atgctggaac
    30121 tcagccgcac cggtctgggg ctggtggcgg tatgtgacgc tcaacaacag gtacaaggcg
    30181 tctttaccga cggcgattta cgtcgctggc tggtaggcgg cggcgcactc accacgccag
    30241 ttaatgaagc gatgacgaca ggcggcacca cattacaggc acaaagtcgc gccatcgacg
    30301 ccaaagaagt tctgatgaaa cgcaaaatca ctgccgcacc agtggtagat gaaaacggca
    30361 aactcaccgg cgcaattaac ctgcaggatt tctatcaggc cgggattatt taatccttca
    30421 atcccagacg tttcgccagc cgatgcaggt tggcgacgtc agtttccagc attcgcgcac
    30481 aggctgccca gttgtgatga ttttgtgcca gtgcctggcg aatagtttca cgctggaacg
    30541 cttctgtcgc ttcacgcagg ttttgcttaa caacgggcac cgccgccgct tctggcggcg
    30601 gcaacgtcac ctcaggaaaa gcaaaatgtt gcgcctcaag aatcacttca tcgccgttgc
    30661 gggtggctct cgccagcact accacccgat gaatagcatg ttccagttcg cgcacgtttc
    30721 ccggaaaact gtagtgttgc agtaaatttc gcgccccggc acttaatacc acgcgggaga
    30781 gcccctgccg caaacgacac tgctcgcaga aataccccgc cagcagaatg acatcatcgc
    30841 cccgctcacg cagcggcggc accgaaagtg gaaacacgct caggcgatga aacaaatcgg
    30901 cgcggaatcg ccctgccagc acctcttcgc gtaagtcgcg gttagtcgcc gccagcacgc
    30961 gcacatcgac ccgcaaacta cggtcatcgc caacgcgctg aatatcgcca tactgcaaca
    31021 ccctcagcag cttggcctgc aatgccaacg acaactcgcc gatctcatcg agaaacagag
    31081 taccgttatc cgccatttca aacttcccgc tgcggttact gatagcgcca gtaaacgctc
    31141 ctttcacatg cccgaacaac tcactttccg ccacactttc cggcagtgcg gcacagttga
    31201 gatagaccag cggattcacc gcccgtggcg aggcttcatg aatcgctttc gccaccagct
    31261 ccttaccggt tcccgtctca ccgctgatca gaacgttgag atcggacgcc gccacaatct
    31321 caatctcttt tttcagttgc gtcatgccag gggacaagcc aatcatctgc gtctgtttca
    31381 ccgcttcaaa cggcgtggca tcgcctggca gcatattctg gctttccagt tgttcaatca
    31441 gcaacgcatt gcttaacgct cccgccgcca gcgcggcaat cagccgtaac tcttcgtcgc
    31501 tgaaaacatc gaactgatcg ggctgcatcc cgtcgagcgt cagtgcgccg atcaggtttt
    31561 gtccgacaaa caatggcaga ccaacgcagg cgtgaacctt cagactctcc tgcccaggaa
    31621 tcaaaccgtc atagggatcg ggcaattcgc tgtctgcggg aaagcgcacc acatccccgg
    31681 cgcgggcaat cgcttccagc cgtggatgcc cttccagcgc aaagcgtcta ccgagtacat
    31741 cctttgccag accgtcgatg gcaagcggaa taaactgccg cgaatcgtaa cgtagcaacg
    31801 cagacgcatc gcactccagc acctgacgta gcgtggtgat caggcgctga aaacgatcct
    31861 gatgaccaat cccacgctgc aattcgatgg cgatattcgc cagcacatca acggaaaaac
    31921 tcatctttgc ctcactgtca atttgactat agatattgtc atatcgacca tttgattgat
    31981 agtcattttg actactcatt aatgagcata attttattta tagagtaaaa acaatcagat
    32041 aagaaactgg cacgcaatct gcaattagca agacatcttt ttagaacacg ctgaataaat
    32101 tgaggttgct atgtctattg tggtgaaaaa taacattcat tgggtgggtc aacgtgactg
    32161 ggaagtgcgt gattttcacg gcacggaata taaaacgctg cgcggcagca gctacaacag
    32221 ctacctcatc cgcgaagaaa aaaacgtgct gatcgacacc gtcgaccata aattcagccg
    32281 cgaatttgtg cagaacctgc gtaatgaaat cgatctggcg gatatcgatt acatcgtgat
    32341 taaccatgca gaagaggacc acgccggggc gctgaccgaa ctgatggcgc aaattcccga
    32401 tacgccgatc tactgtactg ccaacgctat cgactcgata aatggtcatc accatcatcc
    32461 ggagtggaat tttaatgtgg tgaaaactgg cgacacgctg gatatcggca acggcaaaca
    32521 gctcattttt gtcgaaacac caatgctgca ctggccggac agcatgatga cttacctgac
    32581 aggcgacgcg gtgctgttca gtaacgatgc tttcggtcag cactactgcg acgagcatct
    32641 gttcaacgat gaagtggatc agacggagct tttcgagcag tgccagcgtt actacgccaa
    32701 tatcctgacg ccgttcagcc gcctggtaac gccgaaaatt accgagatcc tgggctttaa
    32761 cttaccagtc gatatgatag cgacctccca cggcgtggtc tggcgcgata acccgacgca
    32821 aattgtcgag ctgtacctga agtgggccgc tgattatcag gaagacagaa tcaccatttt
    32881 ctacgacacc atgtcgaata acacccgcat gatggctgac gctatcgccc agggaattgc
    32941 ggaaaccgac ccacgcgtgg cggtgaaaat tttcaacgtc gcccgaagcg ataaaaacga
    33001 aatcctgacc aatgtcttcc gctcaaaagg cgtgctggtc ggcacttcga cgatgaataa
    33061 cgtgatgatg ccgaaaatcg ctgggctggt ggaggagatg accggattac gcttccgtaa
    33121 caaacgcgcc agtgctttcg gatctcacgg ctggagcggc ggtgcggtgg atcgcctttc
    33181 cacgcgcctg caggatgcgg gtttcgaaat gtcgctcagc ctgaaagcca aatggcgacc
    33241 agaccaggac gctctggagt tatgccgtga acacggtcgc gaaatcgccc gtcagtgggc
    33301 gctcgcgccg ctgccgcaga gcacggtgaa tacggtagtt aaagaagaaa cctctgccac
    33361 gacgacggct gacctcggcc cacgaatgca gtgcagcgtc tgccagtgga tttacgatcc
    33421 ggcaaaaggc gagccaatgc aggacgttgc gccaggaacg ccgtggagtg aagtcccgga
    33481 taacttcctc tgcccggaat gctccctcgg caaagacgtc tttgacgaac tggcatcgga
    33541 ggcaaaatga gcaacggcat tgtgatcatc ggttcgggct tcgccgcccg ccaactggtg
    33601 aaaaatattc gcaaacagga cgccactatt ccattaaccc tgattgccgc cgacagcata
    33661 gatgagtaca acaaacccga cctcagccat gttatcagtc aggggcaacg tgccgacgac
    33721 cttacccgcc agacggcggg tgaatttgcc gggcagttta atctgcgcct gtttccgcac
    33781 acctgggtga cggatatcga tgccgaagcc catgtggtga aaagccagaa taatcagtgg
    33841 caatacgaca agttagtgct ggcaaccggt gccagcgcct ttgtcccgcc tgtgcctggg
    33901 cgtgagttaa tgctgacgtt aaatagtcag caagagtatc gcgcctgtga aacgcaactg
    33961 cgggatgccc gacgcgtgtt gattgtcggc ggtggtttga ttggtagcga actggcgatg
    34021 gatttttgtc gtgcaggcaa agcggtcacg ctaatcgaca acgctgccag tattctggcg
    34081 tcattaatgc caccagaagt aagcagccgc ttgcagcatc ggttgacgga gatgggcgtt
    34141 catctgctgt tgaaatctca gttgcagggg ctggaaaaaa cggattctgg cattctggca
    34201 acgctggacc accagcgtag tatcgaagtg gatgcggtaa ttgccgccac cggactgcgc
    34261 ccggaaacag ccctggcacg acgcgccggg ctgacgatta atcgcggcgt ttgcgtcgat
    34321 agttatctgc aaaccagtaa tgccgatatt tacgcgctgg gcgattgcgc ggaaattaac
    34381 ggtcaggtat tgccgttcct gcagccgatt caacttagcg ggatggtgct ggcaaaaaat
    34441 cttctcggca ataacacgcc gctgaaacta ccggcgatgc tggtgaaaat caaaacgccg
    34501 gaattaccgc tgcatctggc aggcgaaacc cagcgtcagg atttacgctg gcagattaat
    34561 accgaacgcc agggaatggt ggcgcgcggc gttgacgatg ctgaccagct tcgcgccttt
    34621 gtggtcagtg aggatcggat gaaagaggca tttgtattgt tgaaaacgtt gccggtgtag
    34681 gtgggctact gtgcctaaaa tgtcggatgc gacgctggcg cgtcttatcc gacctacggg
    34741 gacgcatgtg taggccggat aaggcattta cgccgcatcc ggcaagggtg tccaaatgca
    34801 acacgcttta tccgttctgg acttcacccg ctaaccaacg cgccgcagca ataaccccct
    34861 gccccagaga caaaccgcca tcacccgccg gtaaactctg tggaaagagc aatgtgaaat
    34921 cagcgagata atgcgccaga cgtgcacgca gcaaacggtt atgaataacc ccgccgctaa
    34981 ataccagcgt agtgatacca cgcatcgtgg cctgctcacg catcaacgcg gcaaaaccct
    35041 gcgccagcgc atcatgaaac gcccacgcgc gttgattaac cggtgcctgc cagttcagcc
    35101 actgctgcca gaaagtggcg agatccagtt gattgtccac ccgcggcatc gtcaccggat
    35161 gcgtcactcc gtcgcatgag gctgcgagcg cctccagagc acaagccgct tcaccttcat
    35221 aacttaacgt ggctggcgca cagcccagtg ccgccgccac tgcatcgaac aaccgcccac
    35281 acgatgatgc cagcggcgcg ttaattccac gctcaatggc ccgcgccagt acgctccagt
    35341 tttgctgttg cacacttgcc gtttcagggt aattctgcca ctccggcaca aagcgcaggc
    35401 actgcgccag caggtttcgc cacggctgct tcgccgccaa atcgccaccc ggaagcgcca
    35461 ctgcaggcaa gccgcccagg tgctcacatt cgcgatagtt cacccgcagg cactcgccgc
    35521 cccacaaagc gccattctcc cccataccga taccgtcgag cgtcaaagca atgacatcac
    35581 cgccatccag cggccactga tgctctgcca gacacgccgc tgcatgggca tgatgatgca
    35641 gtaccgtttg cgtcggcaga ttcatttcgc tcgcccactg gctggagaca tagcccggat
    35701 gcacatcatg cacaatgtat tgcggagtga aatcgtagat gttttgcatc aggcgtaacg
    35761 cttcgcgcca ctgcatctgg atgccatcgt cacttaaatc gcccagatgc tgactcaaca
    35821 ccgcttgttc accgcgcacc aggcagaagg tatttttcag atccgcgcca agacacagca
    35881 caggcggaac atttttaaag cccggaggca aaaccagcgc atccggcaca tacccccgcg
    35941 aacggcgcag catttcgccg ctttcgcgca ccactgaatc atccatccgc tgcacgatgt
    36001 cgcggttatg tagcaagaat ccgtcggcaa tgccctgcaa atccgccagc gcctgttcgt
    36061 tgctgatagc tggtggttta ccgctcaggt tgccggaggt catcaccagc gggcattgca
    36121 gttcctgtaa cagcaaatgc tggagcgggt tcgcaggcaa catcaccccg acttcgttaa
    36181 ggccaggggc gatatcatca caaagctcag gtacgtattt tttatccacc agcacaatcg
    36241 gcgcagcggg cgtggtaagc aactggcgcg cagcgtctgg taaactttct gccactggca
    36301 acatgactgc cagcggtttc gccgggcgat gtttgcgctc ccgcagtgtc gccaccgcgt
    36361 tactgttacg tgcatcgcag gcaagatgaa atccgccaat ccctttgatg gcgacaatgt
    36421 tgcccatttt taactgtgcg atagctgcct gtaatgccgc ctcttgttct gcatgttcac
    36481 catgacttac ccattcaaga tgcgggccac actccgggca ggccaccggc tgggcgtgga
    36541 agcgacgatc gagcgggtcg cggtactctt tgtcacaggc cggacatagc ggaaacgccg
    36601 ccatcacggt aaacgggcgg tcgtaaggca tcgcgcgaat aatggtgaaa cgcgggccgc
    36661 agtgggtaca gttgataaac ggataacgat aacgccgctc gcctggggta ttcatttcgg
    36721 caaggcaagc agggcaagtg gcggcatccg ggacaatttg cgtattcatg gtgccgccgg
    36781 tgctctggcg gatggtgaac tcggtgggca gttgtgacca gataaacggc tcacgctcga
    36841 cgctatcaat acgcgccagt ggcggacagt gctgatgcaa ttgaacaaga aacgtttccg
    36901 gatcttccag cagccggact tctacgccat cgccgtcatt acatacatcg ccgtgaagat
    36961 ttaattgctg tgccagctgc cagacaaacg gacgaaaacc gacgccctgc actttgccac
    37021 gaatacgcag ttggacaccg caagatgtgt tttttgccat tgagttattc ccgccatcat
    37081 gaattgcgta acccgccctg ccggacacga cagcgtcgta tccggcagtc tcaggtcggc
    37141 gataccgccc gctccgtatt ctacgaatat ttccgggaat tcctttgatg ccagaacagt
    37201 tctgtaagat ttttagaaca tcagcgccgt acggcggcgt ttttctgcgc tcagttgttc
    37261 aagtttatta cgatcgacac aaatcagcgc atgggtcggg caagccgcca tacacgccgg
    37321 gccgtcttca cgatggttgc acaggtcgca tttattggct tcggctttgt cagcccgtac
    37381 attcagaccc gcgccgctgt tgcgaatcac aggacgtacc accacttcca tcgcgccata
    37441 cgggcaagcc accacgcagg ttttgcaacc aatgcaacgt tcctgcatca catgaacaaa
    37501 ccctttatca cggctgatag caccattcgg gcagacgtta gcgcacggtg catcttcaca
    37561 ctgacggcaa actgtcgccg tggaaatgtt cacaccttta atgacatgga tacgcggtaa
    37621 aaatgtttcc ggggtcagcg atgcacagtc ctgattttcc tgatgagaaa ccacgcacgc
    37681 cacttcacag gtacggcaac caatacattt actcgcgtca gcaatgatga aacggttcat
    37741 caaattctcc agcaatgaca gttaatgcgc cgatacattc acaaatcatg ccagttttta
    37801 atttgctgtt atttaaggaa attaatttct gtaatgcagg aaaaaagatg tcatcgacac
    37861 tagtgacgat gacatgtgat gacaatgttt atcgcgaaag ggcaatgagt gagtcgcggc
    37921 ggatcagttt tccgctaaag gttttcggcg gtgagaaatc cccgccatcg agcataaaaa
    37981 tcagccgtcc aataatttcc tgaatcatct cagtcaccgg aatttttacg ctggagagcg
    38041 ccggaacgat atagggggca attgcgatat catcgaatcc gataactgac acctgctctg
    38101 gcaccgctac gccgcgctcg tgcaacgctt tgatcgcacc tatcgccata tcgtcgttac
    38161 tggcaactaa cgcgctaaat ttagccccac gttcgagcaa cgtttctacc ccttcggccc
    38221 cgctggcggg cgtccattta ccgttagcga taagtttttc attgagcgca ataccatgct
    38281 gcgacagcgc gtctttatac ccggcaagac gttcaatgct ggtgggggaa tccatcgagc
    38341 cggtaaggaa agcaatctcc tgatgcccgg cgtttatcaa ctctgccacg gcgttaaagc
    38401 tggtctgttt atgatcgcac cagacgctat ggctgctatt attgcgcagg cggcgattaa
    38461 gcaccattat cggctgactg tgcgcgtcaa tgatgtcatc aatctcatcc acgcttaaaa
    38521 aacgcgggta aatcataatg gcgtcgcagc gcaaatccag cagatactga atcgcctggc
    38581 gctcttcttc ggcgctgtgt ttaccatctg ccaatagcaa ctgtcgccct ttctcttccg
    38641 ccattcgcgc ggcatgaaac agtaattcac taaaataaat gccgtgataa agcgtgttgg
    38701 tcactaccag ccccagcgtc tgagtactct tcgccgacag attgcgggcc agcaaatttg
    38761 ggcggtaact gctctcttct accgcctgaa acacgcgatc tttagtctcc tggctgacgt
    38821 agccattacc tgaaagcacg cgggaaacgg tcgcttttga aaccccggcg cgcttcgcca
    38881 cttccagcat cgtcgtcatc attttcatcc ctttacacgc aatcaacgca gtgtactgca
    38941 ccgtttgccg attgtccttg cgcaatcggc gggaaaaata ttcaggtgac cggtttcaca
    39001 aatataaaaa atgaacaatt aactctcttg cttatttagt gacaactatt catgattttg
    39061 tgaaaccggt ttcttaattc cgtttcagca tcggtatttt tccgtcacgt cgactgataa
    39121 caactacatc taccctactg ataacaggat aaaatccgat ggccaaaaat tatgcggcgc
    39181 tggcacgctc ggtgatagcg gcactgggcg gcgttgataa catctcggca gtcacgcact
    39241 gtatgacgcg tttgcgcttt gttatcaaag atgaccagct tatcgacagc ccgacgttaa
    39301 aaaccatccc cggcgtgctc ggcgtggtac gtagtgacaa ccagtgtcag gtaattatcg
    39361 gcaatacggt ttcgcaggcc tttcaggaag tcgtcagcct gctgccggga gatatgcagc
    39421 ccgcacagcc cgtgggtaaa cccaaactca cgctgcgtcg cattggtgcg gggatcctcg
    39481 atgcgctgat cggcaccatg tcaccgctga tcccggcgat tatcggcgga tcgatggtca
    39541 aactgctggc gatgatcctc gagatgagcg gcgtgctgac aaaaggatcg ccgaccttaa
    39601 ccattctgaa tgtgattggt gacggtgctt tcttcttcct gccgctaatg gtcgcggcat
    39661 ctgccgccat caaatttaaa accaatatgt cgctggcgat tgccatcgcg ggtgtgctgg
    39721 tacatccgag ttttattgaa ttaatggcga aagcggccca gggtgaacat gtcgaatttg
    39781 ccctgatccc ggtcaccgcg gtgaaataca cctacacggt gatcccggcg ctggtgatga
    39841 cctggtgcct gtcatatatc gaacgctggg tggatcgcat tacaccggcg gtgaccaaaa
    39901 acttcctcaa gccaatgctg attgtgttga ttgccgcacc gctggcaatc ctgctgattg
    39961 gcccgattgg tatctggatc ggtagcgcca tttcggcgct ggtttacacc attcatggtt
    40021 atctgggctg gctttcagtc gccattatgg gcgcgctgtg gcctctgctg gtaatgaccg
    40081 ggatgcaccg cgtctttacg ccaaccatca ttcagaccat tgccgaaacc ggcaaagaag
    40141 gaatggtcat gccatcagag atcggcgcta acctgtcgct gggcggttca tcactggcgg
    40201 tgccgtggaa aacgaaaaac ccggaactgc gccagacggc gctggctgcg gcggcatcag
    40261 ccattatggc ggggatttcc gaaccggcgt tatacggcgt ggcgatccgc ctgaaacgtc
    40321 cgcttatcgc cagtcttatc agcggtttta tttgcggcgc ggttgccggt atggcggggc
    40381 ttgccagcca ctcaatggca gcgccggggc tatttaccag cgtgcaattc ttcgatccgg
    40441 cgaatccaat gagcatcgtc tgggtgttcg cggtcatggc gctggcggtg gtgctgtcgt
    40501 ttatcctcac actgttgctc ggctttgagg atattcctgt tgaggaagcg gctgcccagg
    40561 cgcgaaagca tcagagcgta caaccgaccg tcgccaaaga agtaagtctt aattgaggat
    40621 gaaaatgtca gtatttccag aaggtttttt atggggcggc gcgcttgccg ccaaccagtc
    40681 tgaaggtgcg ttccgtgaag gcggaaaagg gctgaccacc gtcgatatga tcccacacgg
    40741 cgagcatcga atggcggtga aactggggct ggaaaaacgt tttcagttgc gcgatgacga
    40801 gttttatccc agccatgagg cgacggattt ttatcatcgt tataaagaag atatcgccct
    40861 gatggcagag atgggattca aggttttccg tacctcaatt gcctggagcc gtctctttcc
    40921 gcaggacgat gaaatcacgc ccaatcagca gggcattgct ttttatcgtc ctgtctttga
    40981 agagtgtaaa aagtacggta tcgaaccgct ggtcacgttg tgccacttcg atgtgccgat
    41041 gcatctggtc accgaatatg gctcctggcg taaccgcaag ctggtggagt ttttcagccg
    41101 ctacgccaga acctgctttg aagcatttga tggtctggtg aaatactggc taaccttcaa
    41161 tgaaatcaac attatgttgc atagcccgtt ctccggcgcg ggtctggtgt ttgaagaagg
    41221 tgaaaatcag gatcaggtga aatatcaggc cgcgcatcac cagctggttg ccagtgcgct
    41281 agccaccaaa atcgcccatg aggttaaccc gcaaaatcag gtgggctgta tgctggcggg
    41341 cggtaacttc tacccttaca gctgcaaacc ggaagatgtc tgggcggcgc tggagaaaga
    41401 tcgggaaaac ctgtttttta tcgatgtgca ggcgcgcggc gcgtatccgg cttactctgc
    41461 ccgcgtattt cacgaaaaag gggtaaccat agataaagcg ctgggcgatg atgagatcct
    41521 gaaaaacacc gtcgattttg tctctttcag ctattacacc tcgcgctgcg cctcggcgga
    41581 gatgaacgcc aacaacagca gtgcggcgaa cgtggtgaaa tcgctgcgta acccgtatct
    41641 gcaagtgagc gactggggct ggggaattga tccactcggt ctgcgtatca ccatgaatat
    41701 gatgtacgat cgttatcaga agccgctgtt tctggtggaa aacggcctcg gcgcaaaaga
    41761 tgaacttgct gccaatggcg agattaacga tgactatcgc atcagctatt tacgcgaaca
    41821 tatccgcgca atgggcgaag cgattgcaga tggcattccg ctgatgggct acaccacatg
    41881 gggctgtatt gatttagttt ccgcctctac gggtgaaatg agcaaacgct acggttttgt
    41941 ctacgtagac cgtgacgacg caggcaacgg cacgctgacg cgcacacgta agaaatcgtt
    42001 ctggtggtat aaaaaagtga ttgccagtaa tggggaagat ttagagtaag taacagtgcc
    42061 ggatgcggcg tgaacgcctt atccggccta catagttcgt gcgacatgta ggcccgataa
    42121 gcgcagcgca tcgggcaatt tagcatttgg cattagtcaa aaatcaaaga aaccattccg
    42181 gcttaacgcc aaatcgggct gaaagtgcct taatgtggga tatcgtcagg gatcgttgtc
    42241 cagacaaaat ctgactgacc agggatttag agccgatttc gttcttcaaa tccgcatagg
    42301 tcagattatg ttgatcaatc agcgtgcgaa gtaacgccac gccaacaggc attgccgcaa
    42361 cggctttgtc gaactctgca aactcttggt tgttgttttc atattctgca atgcggctcg
    42421 ccagaaagtc gataagtgga ttggtgtcat cttcttcaat caaatattcg accagttcca
    42481 gtgcttcacg ataatcatct tctgagtcac tacctcccag aaaggggact gcattgacca
    42541 gttctcttgt tgctttaaca gcgtgtgcag cattagcagt cattctttgt tctctctgta
    42601 gtaacgtgtc agtttgtcat actcggcgtg attagttatg tgcttcacaa agaaccgctt
    42661 attaacaaag tttatgtagg caatcacccc taggttgttc ccacctacat ccagaacgta
    42721 ccatttgttg cgatatttaa aattgtcgag gttgggaaac gccgttcgca tctcttctgg
    42781 cgtagaaaaa tccgtttcct taataacccg atacaatgcc tgaagagcca gagcgtcgtt
    42841 aggatattta cgcgcagact cttcaaatgg cgctttagaa atgatgtgca tttttaagtt
    42901 ttaacttcct tatagtaagt aaacagccac atctgcgagt tctatcctta tgtgttcgga
    42961 aactatagct catcaaatcc aagttcacaa tatgtaaaca cttgtgcgcg atataattac
    43021 ccccaaaatc ctcatagcca ctctgcaaaa agtcagttct ggttcacccc tcaagcacat
    43081 ccccctttat caagcaacgc tgcgtctgcg ccagatcttc cgtttgcggg tcgcgcccgg
    43141 cgatgggttt gaggacgagt ttacggttgg gggtataagt aaaacgcgtg agcgcgggat
    43201 agcttaactt gtcaaaccag ccgtgaacac gctgccattt ggcgtctttt acctgataca
    43261 gactggttat ccagtaacca gaattaaact gcatatccag caattgcgtg tgaccattgc
    43321 cttgcaaatc cagcaaatca tctacgccag ttttgcttgc ggtgtagaat cccctgggtt
    43381 caaagacaca gggacggcct tcgcgcgtga acgtcataag aattaaatgt gcataaggag
    43441 ccagaccatt accggtactt ggtaaccaga taaccagatc ctgaatgccg ttacgatcaa
    43501 gatcggcgcg atagagttgc cctttgcccg ctatgtttgc cagcataccc acaggcacag
    43561 tccaggattt accggcgcgg tctttagcgg ttaattgcca gtaatcttct tcatttggca
    43621 tcttctcaag cgtcacgcca gcaacgccca ctttcctaaa atgccaggtt tgctcggcat
    43681 cataaggcat ggtcaggaga ggtttcagcg cccgcttttg cgcgtattcg ctgtcactca
    43741 ttggcagata ttcggtaaag ctggtggcct gtgcaggcag cactaagctc aataaaaaag
    43801 cgcacatccc tgttctgatg ttcaattccc tgaattccct gaattccctg atggagaatt
    43861 attcttcttc caccgctaac tgcgcaaatc caccatttcc ttcccagcct tccagtcgtt
    43921 gataaacggt ttctaccgca tctttaatcg gctgggtcat cgggtagtaa aagccgacga
    43981 tatccggctg aatgccgagg aaaatcactt caccaatgtc ttcttttaac tggtcgataa
    44041 ggtaattcaa cggcatgtta tgggtagtca tcataaacat ctcggcgata tcatccgggt
    44101 cgatgatgcg gatttcgccg gggtttagcc ccatatccgt ggcgtcgaca atcagcagtc
    44161 gtgtcgggcg cagttcacgg atggcgacga tgtcgttttc cggtgcacta ccgccgtcaa
    44221 tcaccaccca gttacctttc ggcgcggcgg cgcacttttc cgccagcagc gggccagcgc
    44281 catcatcgcc catcatgcta ttgccaacac agagtaaaac gtcagtcacg cagtctcctc
    44341 accatcaaat agatggcgtt ttcctgatga atatcatgca gcatacttaa cagcattgta
    44401 ctccattcct gctgctgcga agtttgcgct gcccgggctt tatcgaatgc gttggccagc
    44461 atcggcacat ggttaatgtc gatgacgatc tcgccatatt tcggtacgcc ttccattttg
    44521 tggcgggctt cgctgcccgc ctccagagtg gcaatccatg ccagatattc atcccatggg
    44581 caggtgagcg ccgcttccag gcaatcgata accccaaggt ggtgaccaat cgccaggctg
    44641 taatagacca cctgctgcgc ctcggcgggc gtggcatcgt tctcatcaat aaatttacgg
    44701 ctcagttgac tgaacaccac cttttcactc atcggatacg cgcctcttca acaacatgat
    44761 tcagatggct gacaatctcg ttcagacgcg gatcgttttc cgcttccagc cagcgtgcaa
    44821 cctgttcttc gccctgccct aactgtgtaa ggtaatcatc ggcaatctga cgaccgtaac
    44881 gataacccgc caggcgacgt gcttcgcgat ccactttcac gcgcagcggc tgcaccatat
    44941 cgccatgcag gatctccgcc ggttgttcat ccagttcacc cggcccacgg gcgtgaattt
    45001 tctgctccag caggccgagc gccattgcaa agccgtacag cgtggcggca ggcgttggcg
    45061 ggcagctagg gatataaaca tccacaggga caattttatc cgtaccgccc cacacgcagt
    45121 agagatcgtg taagatcccg ccgctgttac cgcaggcacc gtatgagata cagattttcg
    45181 ggtccggcgc ggactgccac gcacgcagcg caggggatcg cattgcacgg gtgaccgcgc
    45241 cggtaaacag taaaatatcc gcatgacgcg gtgaaggaac gactttaatg ccgaagcgtt
    45301 ctgcatcgaa cagcggagaa agcgtggcga aaatttcgat ttcgcagccg ttgcagccgc
    45361 cgcagtccac gcggtaaaca taggcagaac gtttgatttt tttcagtaac gacgccttca
    45421 tgctggcgat ggattcatcc accgtcatcg ggaccggaat gccgttggcg tcacgggggc
    45481 ctaataaatt gctcatcaga tggcctcttt catatggcga gtcagttcaa tacggtcgga
    45541 cggcaccagg catttctggc gcttacattc cgggcaagtc tcaaagcttt cgcggtggtt
    45601 ttccgcgcgg ctgtcaccat tgtgcttaag cagcgcaatg gcgtagtcga tctctttctg
    45661 tacggcgaaa ggacgattgc agacgcggca gttgcacagc gcgaagcggg actgttgcag
    45721 gaagtcttct ttcttccaca ccgccagttc gtactcttgc gacagtttga tcgccgccgt
    45781 cgggcaaact tcttcgcagc gtccacagaa gatgcagcgc ccgagattaa actcccacgc
    45841 cagctctcct gtggcgaggt cagtttcaac cgttaaggcg tttgacgggc aggcattgac
    45901 gcaagccgcg cagccgatgc actgctgcgg gttctgctct ggcttaccac ggaagttttt
    45961 atcaaccgca atcggctcca gcggataaga cgaggtcgcc gtgccggttt tgatgacttt
    46021 tttgataaag gtaaacatgg cgattcctta tttcagcggc gagtttttac gctcaatgct
    46081 gtaacgctcg agttctttgt acggcaccac tttgctcttc ttcttacgca catcgactac
    46141 ggtcatgcgg tcggtacagg agtagcaagg gtccaggcta ccgataatca gcggcgcatc
    46201 ggaaacggta ttgccacgca gcatgtagcg cagggtcggc cagttcgcgt aggtcgcggc
    46261 acggcagcgc cagcggtaca gcttctggtt gtcgccggtc atgctccagt ggatatcgtc
    46321 gccgcgcggc gcttcggcaa agcccagcgc gaaacggtgc ggaatgtagg taaagccttc
    46381 caccatcagc ggaccgcccg gcaggttatc cagaccgtag tcgatcatat tcagcgcggt
    46441 atagacctcg ttgattcgca ctttcagacg ggaaataacg tcgcagccct gctcgctgtg
    46501 gacttccatt ggcagcaggc cataaccgac aaacgggtga tcggcgcgag tatcgcgggc
    46561 gagaccgctg gcgcgaacca tcgggccaac gttactgaaa tcgcaggcga tttccgggtc
    46621 cagacgacca atgccgacgg tgcgttgttc catgttcggc gtactcagca gcacatccac
    46681 cagctcctgc acttcacgac gcatttgttg cgccagctgg cgggtctgga tcatgtcatc
    46741 tttcagcaga tcgtgacgaa tcccgccgat caggttcagg ccgtaggttt tacgcgcacc
    46801 ggtaaggatc tctgccattt tcatggaggt ttcacgcacg cggaagaact gcataaagcc
    46861 ggagtcgaag ccggtaaagt gacaggcgag gccgaggttg agcagatgcg agtgcaggcg
    46921 ttccacctcc agcagaatgg cgcggatcat ctgcgcgcgt tctggcacct gaatacccat
    46981 cgcgttttcc accgacgtgg tgtaggcggt gctgtgggca aagccgcaga tcccgcacac
    47041 acggtcagag aggaaggtca cttcgttata acccatgcgg gtttccgcca gtttttccat
    47101 accgcgatgg acgtagaaca gacggtagtc ggcgtcgata atgttttcgc catcgacgaa
    47161 cagacggaag tgacccggtt cgtcagaagt gacgtgcagc ggaccaatcg gcacgacgtt
    47221 gtttttcttg tcgcccagtt cgttgatgaa ctcgtaggtt tcagcatcgg tagtcggtgc
    47281 cggacgctga cgataatcca tgctgtcttt acgcagcgga taaagttcat ccggccagtc
    47341 atccggcaga ccaaccggaa tcaaaccgta catatcgcgc acttcgcgct cgccccacac
    47401 cgccgccgga acgcgcggcg tcacggacgg atactccggt ttgttggcgt caacttcgac
    47461 gcgaaccgtt acccaacact tggtgccctt ctccatcgac agcacgtaat aaacggcgta
    47521 atgaccattc agtttgcgtt cgtcgttacc aaacagcacc gacagccagc caccctgctt
    47581 gtagtagaga aactccacca cttccggcag atagttcacc tttacggtga cagtcagctg
    47641 atctttggtc tgccaggcgt ggtccagcac gacgcctgga aatgcctcat tcagcgcggc
    47701 gagataatgt tgacctaatt tttcttcaga catgctcaaa ctctctttaa tcacgccgcc
    47761 agcaaggaga cgaacgctaa aaatgcaaag ccaaacccag cccaggtaat gcgcggagta
    47821 atatcaagac gcagacgggc catactgttt tcgaacagcg cgataaccag gacgccgacc
    47881 accagtttga cgatggcaat caccagcgcc agcagcagtc caccgacggt gaaggtttcc
    47941 atttgtcccc acggaataaa caccccgacg aacatctgca acaccaccag ctgtttcagg
    48001 ctgatacccc atttcatgac gccaaagccg ctaccgctgt attcggagag cggaccttcc
    48061 tgcaactcct gctcggcttc cgccaggtcg aacggcagtt tgcccatttc gataaaagtg
    48121 gcgaacgcac aggcgcagag cgccagcacc agcgggatgc tttggctcag cggccagtga
    48181 taaacggtgt cggtaatgtt gctgatgttg gtggaaccgg caacctgtgc ggcaacccac
    48241 agaccaagca gcaacatcgg ttcgaccagc acgccaagca tcgcttcacg gctcgcaccg
    48301 atagcggtaa acgggctacc ggtatccaga ccggaaatgg caaagaagaa acgcgcgatg
    48361 gcgaagagat acagtaaggt gatcaaatcg cccagttgcg gcagcggaga accgatagtc
    48421 agcatgacgc ccaccatcac atacggcgtc aggcggaaca tccagccaga ggcatccggg
    48481 ccaacgctct ggcggcccag cagtttgata atgtcgcgat actcctgcaa cacgcccggc
    48541 ccgcgacggt tatgcagacg ggcgcgcgcc acgcgggtaa taccggagag cagcggcgca
    48601 acggcaaata acaccagcgc ctgaattaac ggatataaaa cactcattct caggctcctc
    48661 gtgaaacaat aatcaccacc agtaccgcca gttcaaccag cgccatccgg cggaacagca
    48721 acgcactacc ttcgcactgc cagcccggca ccagcgacac cggattcagc catttgcgca
    48781 gtttcagcac tggcgcaaac gcctgtttaa ccggcatggc aaaaccgtga gcggtaatca
    48841 ccattgattt ttcatggtcg tagccgcaca cccaggccgc accgcgggaa cgtgacggca
    48901 ggcggtcgcc tttgcaaatc gccataatga tgaatggcag cagcgggcag gcaatcagca
    48961 gcaatgtgat cattggttga gaaacggtgg tgttagcagg ctccagaggc agaggtacag
    49021 cagcagagag catcggcagc agccacggcg cagcaacacc gccaattacg cagcaaatcg
    49081 ccagtgccac tacgcttacg ctcatcagga gcggcgcaca ggtggcgttt tcggcttctt
    49141 tggtacgcgg cgcgccgagg aacgtgacgc catagacttt cgccatacac atcaccgcca
    49201 gcgcaccggt aattgccaga cccacagcga gcagcggccc cagcagacgg gcaacaaacg
    49261 cgccgctatt gctcagtttg aagaaggatt gatagataac ccattccccg gcaaacccgt
    49321 tcagtggcgg cagcgcagcc attgccatca acccgactaa catggcgatg gagataaccg
    49381 gcattttctt gccaatacca ccgagttttt cgatatcgcg atgaccggta cggaaccaga
    49441 cgctccccgc cccgaggaac agtacgcttt tgaacaggct atggttaagc agatggtaca
    49501 gaccaccgac caggccaaga gcaatcagcg ccggttgttc gagcgcgata cccgttacgc
    49561 cagcgcccag ccccagcccc agccccagcc ctagccctag ccctagccct agccctagca
    49621 ggataatgcc gatattttcc agggtgtggt aagccagcag gcgctggatg ttgtgttcca
    49681 tcaacgcata cagaccaccg acgaacgcgg tgatcatgcc gagcaccagc agcgcgatcc
    49741 cccaccacag cggtgcatta ccgcccagca gtgacagggt taacatgccc agcaggccaa
    49801 ttttcatgac taccgtagaa aacaacgcgg cagctggcgc agaggcgtta gcatgtgcct
    49861 gcggcaccca gccgtgcagc ggaataatcc cagccagcag gccaaagcca atcacaccga
    49921 gcagccagat atcggaaccg agcagccaga tatcggaacc gagcggcagc tgttgcatac
    49981 gcatatccag caggcgcaga tccagcgtgc cataacgctg ccacagcagc cagcaagcaa
    50041 tcaccagcag cagagtgcca agacgcccca gcgcaaacca cagtttgccc tctttgctgt
    50101 tgctggtgag gaacaccgca cacaaggcca tgacttcggc cattaccacg aacatgccga
    50161 ggttgctggc gatgacggcg cagacggcgg cagccatcaa catattgatc tgcaagccgt
    50221 tgcacttcac ctgcgcgtgg cgatgccagt caatgttata gaggctgaca aacagaccgc
    50281 aaaggccgag ggtaatcagc caaatcgcgt taagcgggga gatttgcaca tcgtagctta
    50341 ccagcgacat cgcaccgctc acgccaaccg agccagtcag tacagtgaag cccgcggctg
    50401 ccgtatacag actaccaacc gcgccgccaa ttccggctat ccagccactt agcgcttttt
    50461 gaaaagaaaa gagaaatgcc agaacagcgg cggcgacaaa ccatgccacg ccgctattga
    50521 tcagggaaat tgcgctcatt tagcctctcc actttgagcc tgctgaaaca aggtgagatc
    50581 gccaaagtcc gtgttaaaag tcagctcacg cttacgtttg ctgacgcggg cgatatcggt
    50641 gttatccacc agatgcaggg ctttagtcgg gcacatccgc acacaggccg gaccttgttc
    50701 atcaaagcta caaaggtcac atttgacggc gatcgcgcga atacctggca cccagtcaag
    50761 caatgtgctg acacgcgccg gagcaggcgg tgccggtggc gctttcgggg tattggcgtt
    50821 tgccggaata tccagcggac ggctgccgga aaattcaatt gcgccaaacg ggcaggcgat
    50881 gccgcacagt ttgcagctta cgcacagact ttcattcaac tgcacggccc catcaacgcg
    50941 ggtgatggcg ttaaccgggc agaccaccgc gcagggtgca tcttcacagt ggtgacagag
    51001 cagcggcgca gattcttttt cattgagcat cactctcagg cgcggcattg attgcaggcc
    51061 gtgctggcga tgcgtctctg aacaggcggc ctcacaggtg tggcagccga tacagagcgt
    51121 ggagtcggca attacaaaac gattcaccag gcattcctca ggtgattgtc atttttgacg
    51181 aaaacatgcc gatgaaatgt cattttcgac actcatcgac acgcccatcc ccaaacaggc
    51241 gtaacgcctg caaaacgggc aaagcctcag ctcatgctgc cgggctttgt ccctttacca
    51301 gttggcttaa attcaccggc acattgtttt caatggcggt gtataagctg tcgtaaacgc
    51361 cagcgatttt ttcgagatag tgttccagaa cctgttcgcg atcgcggatg ttgaccttgc
    51421 catcaatcat gccgtcaatg ctcagttgcg cctgcgcaat ccgctgtttg tcttcgccat
    51481 cttttgtttc gacgtaatac tcgatggtgt ggctgttaaa gccatccgcc agggtgacgt
    51541 aaatacgaaa atgttcaaaa aggacgaaac agagttcttt gtccggcgcg cagctcatgg
    51601 cgtgatgcag gcgcgctttc gataacgtga tcccctgaac cagcgaattg cagtagatgt
    51661 gccactggtc ctgtaggcga cgatgccgct gtgcgatgta atcggctttc tcgcttattt
    51721 cccaaatagt cattgtcagg ttactcgttt aacagagatg ccagctttaa gcattttttg
    51781 taccaacttt taatttattg ttattaaaga gatttttaag ctaaagatga atttcgtcgc
    51841 cgtgtcgacg tgtcatttcg acatcatcga cattattcac cgcagggata atcaacactg
    51901 gcgcaattat tgcttgtagc tggcaatagt taatgggagg cgatatgcac gaaataaccc
    51961 tctgccaacg ggcactggaa ttgatcgaac agcaggccgc aaaacacggc gcaaaacgcg
    52021 taactggggt ctggctcaaa attggcgcat tttcttgtgt cgaaaccagc tctcttgcct
    52081 tttgttttga tctggtttgc cgcggcagcg tggcggaagg ttgtaaactg cacctcgaag
    52141 aacaagaggc cgaatgctgg tgtgaaacat gccaacagta tgtgacgcta ctgacccagc
    52201 gcgtccgccg ctgtccacag tgtcatggtg acatgctaca gattgtggca gacgacggtt
    52261 tacagattcg gcggatagaa atagaccagg agtgagcgat gtgtacaaca tgcggttgcg
    52321 gtgaaggcaa cctgtatatc gagggtgatg aacataaccc tcattccgcg tttcgtagcg
    52381 cgccatttgc cccggcggca cgcccgcaga tgaaaatcac cggcattaaa gcgcctgaat
    52441 ttacccccag ccagactgaa gaaggcgacc tgcattacgg tcatggcgaa gcgggcactc
    52501 acacaccggg catgagccag cgtcggatgc tggaagtcga aattgacgtg ctggacaaaa
    52561 acaaccgtct ggctgaacgc aaccgcgcgc gctttgctgc ccgcaagcaa ctggtgctca
    52621 acctggtttc cagccctggt tccggtaaaa ccaccctgct gacggaaacc ttaatgcgcc
    52681 tgaaagacag cgttccgtgc gcggttattg aaggcgacca gcaaaccgtg aacgatgccg
    52741 cacgcattcg cgctaccggt acaccagcga ttcaggtgaa caccggtaaa ggctgccatc
    52801 ttgacgcaca gatgattgcc gacgccgcac cgcgtctgcc actggacgat aacggtattc
    52861 tgtttatcga aaacgttggc aacctcgtat gcccggccag cttcgatctc ggtgaaaaac
    52921 acaaagtagc ggtgctttcc gttaccgaag gtgaagacaa accgctgaaa tatccgcata
    52981 tgtttgccgc cgcctcgctg atgctgctca acaaagttga cctgttgccg tatctcaact
    53041 ttgacgttga gaagtgcatc gcctgcgccc gcgaagtcaa tccagaaatt gaaatcatcc
    53101 ttatttccgc caccagcggc gaagggatgg accagtggct gaactggctg gagacacagc
    53161 gatgtgcata ggcgttcccg gccagatccg caccattgac ggcaaccagg cgaaagtcga
    53221 cgtctgcggc attcagcgcg atgtcgattt aacgttagtc ggcagctgcg atgaaaacgg
    53281 tcagccgcgc gtgggccagt gggtactggt tcacgttagc tttgccatga gcgtaattaa
    53341 tgaagccgaa gcacgcgaca ctctcgacgc cttacaaaac atgtttgacg ttgagccgga
    53401 tgtcggtgcg ctgttgtatg gcgaggaaaa ataatgcgtt ttgttgatga atatcgcgcg
    53461 ccggaacagg tgatgcagtt aattgagcat ctgcgcgaac gtgcttcaca tctctcttac
    53521 accgccgaac gccctctgcg gattatggaa gtgtgtggcg gtcataccca cgccattttt
    53581 aaattcggcc tcgaccagtt actgcctgaa aacgttgagt ttatccacgg tccgggttgc
    53641 ccggtgtgcg tactgccgat gggtagaatc gacacctgcg tggagattgc cagccatccg
    53701 gaagtcatct tctgtacctt tggcgacgcc atgcgcgtac cagggaaaca gggatcgctg
    53761 ttgcaggcaa aagcacgcgg tgccgatgtg cgcatcgttt actcgccgat ggatgcgttg
    53821 aaactggcgc aggagaatcc aacccgcaaa gtggtgttct tcggcttagg ttttgaaacc
    53881 accatgccga ccaccgccat cactctgcaa caggcgaaag cgcgtgatgt gcagaatttt
    53941 tacttcttct gccagcacat tacgcttatc ccgacgctgc gcagtttgct ggaacagccg
    54001 gataacggta tcgacgcgtt cctcgcgccg ggccacgtta gtatggttat cggcactgat
    54061 gcctataatt ttatcgccag cgattttcat cgtccgctgg tggtcgctgg tttcgaaccc
    54121 cttgatctac tgcaaggcgt ggtcatgctg gtggagcaga aaatagcggc ccacagcaag
    54181 gtagagaatc agtatcgtcg ggtggtaccg gatgccggta acctgctggc gcaacaggcg
    54241 attgccgatg tgttctgtgt caacggcgac agcgaatggc gcggcttagg cgtgattgaa
    54301 tcttctggcg tgcacctgac gccggattat caacgattcg atgccgaagc acatttccgc
    54361 ccggcaccgc agcaggtctg cgatgacccg cgcgcgcgtt gtggtgaggt attaacgggc
    54421 aaatgtaagc cgcatcaatg cccgctgttt ggtaacacct gtaatcctca aaccgcattt
    54481 ggtgcactga tggtctcctc cgaaggagcg tgcgccgcgt ggtatcagta tcgtcagcag
    54541 gagagtgaag cgtgaataat atccaactcg cccacggcag cggcggccag gcgatgcagc
    54601 aattaatcaa cagcctgttt atggaagcct ttgccaaccc gtggctggca gagcaggaag
    54661 atcaggcacg tcttgatctg gcgcagctgg tagcggaagg cgaccgtctg gcgttctcca
    54721 ccgacagtta cgtcattgac ccgctgttct ttcctggcgg taatatcggc aagctggcga
    54781 tttgcggcac cgcgaatgac gttgcggtca gtggcgctat tccgcgctat ctctcctgtg
    54841 gctttatcct cgaagaagga ttgccgatgg agacactgaa agccgcagtg accagcatgg
    54901 cagaaaccgc ccgcacggca ggcattgcca tcgttactgg cgatactaaa gtggtgcagc
    54961 gcggcgcggc agataaactg tttatcaaca ccgctggcat gggcgcaatt ccggcgaata
    55021 ttcactgggg cgcacagacg ctaaccgcag gcgatgtatt gctggttagc ggtacactcg
    55081 gcgaccacgg ggcgactatc cttaacctgc gtgagcagct ggggctggat ggcgaactgg
    55141 tcagcgactg cgcggtgctg acgccgctta ttcagacgct gcgtgacatt cccggcgtga
    55201 aagcgctgcg tgatgccacc cgtggtggtg taaacgcggt ggttcatgag ttcgcggcag
    55261 cctgcggttg tggtattgaa atttctgaat cagcgctgcc ggttaaacct gccgtgcgcg
    55321 gcgtttgcga attgctggga ctggacgccc tgaactttgc caacgaaggt aaactggtga
    55381 tcgccgttga acgcaacgcg gcagagcaag tgctggcagc gttacattcc catccactgg
    55441 ggaaagacgc ggcgctgatt ggtgaagtgg tggaacgtaa aggtgttcgt cttgccggtc
    55501 tgtatggcgt gaaacgaacc ctcgatttac cacacgccga accgcttccg cgtatatgct
    55561 aataaaattc taaatctcct atagttagtc aatgaccttt tgcaccgctt tgcggtgctt
    55621 tcctggaaga acaaaatgtc atatacaccg atgagtggtc tcggacagca agggttgttc
    55681 gacatcactc ggacactatt gcagcagccc gatctggcct cgctgtgtga ggctctttcg
    55741 caactggtaa agcgttctgc gctcgccgac aacgcggcta ttgtgttgtg gcaagcgcag
    55801 actcaacgtg cgtcttatta cgcgtcgcgt gaaaaagaca cccccattaa atatgaagac
    55861 gaaactgttc tggcacacgg tccggtacgc agcattttgt cgcgccctga tacgctgcat
    55921 tgcagttacg aagaattttg tgaaacctgg ccgcagctgg ccgcaggtgg gctatacccc
    55981 aaatttggtc actattgcct gatgccactg gcggcgggag ggcatatttt tggtggctgt
    56041 gaatttattc gttatgacga tcgtccctgg agcgaaaaag agttcaatcg tctgcaaaca
    56101 tttacgcaga tcgtttctgt cgtcaccgaa caaatccaga gccgcgtcgt caacaatgtc
    56161 gactatgagt tgttatgccg ggagcgcgat aacttccgca tcctggttgc cattaccaac
    56221 gcggtgcttt cccgcctgga tatggacgaa ctggtcagcg aagtcgccaa agaaatccat
    56281 tactatttcg acattgacga tatcagcatt gtcttacgca gccaccgtaa aaacaaactc
    56341 aacatctact ccactcacta tcttgataaa caacatcccg cccacgaaca gagcgaagtc
    56401 gatgaagccg gaaccctcac cgaacgcgtg ttcaaaagta aagagatgct gctgatcaat
    56461 ctctacgagc gggacgattt agccccctat gaacgcatgt tgttcgacac ctggggcaac
    56521 cagattcaaa ccttgtgcct gttaccgctg atgtctggcg acaccatgct gggcgtgctg
    56581 aaactggcgc aatgcgaaga gaaagtgttt accactacca atctgaattt gctgcgccag
    56641 attgccgaac gtgtggcaat cgctgtcgat aacgccctcg cctatcagga aatccatcgt
    56701 ctgaaagaac ggctggttga tgaaaacctc gccctgaccg agcagctcaa caatgttgat
    56761 agtgaatttg gcgagattat tggccgcagc gaagccatgt acagcgtgct taaacaagtt
    56821 gaaatggtgg cgcaaagtga cagtaccgtg ctgatcctcg gtgaaactgg cacgggtaaa
    56881 gagctgattg cccgtgcgat ccataatctc agtgggcgta ataatcgccg catggtcaaa
    56941 atgaactgcg cggcgatgcc tgccggattg ctggaaagcg atctgtttgg tcatgagcgt
    57001 ggggctttta ccggtgccag cgcccagcgc attggtcgtt ttgaactggc ggataaaagc
    57061 tctctgttcc tcgacgaagt gggcgatatg ccactggagt tacagccgaa gttgctgcgt
    57121 gtattgcagg aacaggagtt tgaacgtctc ggcagcaaca aaatcattca gacggacgta
    57181 cgtttaattg ccgcgactaa ccgcgatctg aaaaaaatgg tcgccgaccg agagttccgt
    57241 agcgatctct attaccgcct gaacgtattc ccgattcacc tgccgccact acgcgagcgt
    57301 ccggaagata ttccgctgct ggcgaaagcc tttaccttca aaattgcccg tcgtctgggg
    57361 cgcaatatcg acagcattcc tgccgagacg ctgcgcacct tgagcaacat ggagtggccg
    57421 ggtaacgtac gcgaactgga aaacgtcatt gagcgcgcag tattgctaac acgcggcaac
    57481 gtgctgcagc tgtcattgcc agatattgct ttaccggagc ctgaaacgcc gcctgccgca
    57541 acggttgtcg cccaggaggg cgaagatgaa tatcagttga ttgtgcgcgt gctgaaagaa
    57601 accaacagcg tggttgccgg gcctaaaggc gctgcgcaac gtctggggct gaaacgcacg
    57661 accctgctgt cacggatgaa gcggctggga attgataaat cggcattgat ttaactgcaa
    57721 attgccggac agatctgcct gtccggcata ctattcacga ggttttttcg gacgatattt
    57781 ttccggcagt tctggcaccg gacgcttgtc atcgatgaga tgacgcacgg ttaagatcgg
    57841 atgacgccac agcattctcg gcccggccca acgcataatc tgtttcatct cttcacgctt
    57901 tgcaggctgg taacagtgca ccggacactg cttacaggct ggtttctctt cgccgaacac
    57961 acatttatcc agccgctttt gcgcgtaaac aaacaacgcc tcgtaatgct ccggctccgc
    58021 tgacgcctgc gggcatttcg cttgataaag atcgatcatt tttttaatcg tcagtttttc
    58081 acgagagata cgcttgccgg acatgctgcc tccacctcat taagatgcat ttatattaca
    58141 tcttaatctt aaagggcact atgactctaa agaagaaggg ttcgccaatc gatacaattt
    58201 tgcgtacttg cttcataaac atcacgcaaa agctgcaaaa cagcatcttt cccggaacca
    58261 gcatcaagaa ctcgccgttc gcttcttccc ctgaaatgat taactccggt atcatgtgcg
    58321 ccttatgtga ttacaacgaa aataaaaacc atcacacccc atttaatatc agggaaccgg
    58381 acataacccc atgagtgcaa tagaaaattt cgacgcccat acgcccatga tgcagcagta
    58441 tctcaagctg aaagcccagc atcccgagat cctgctgttt taccggatgg gtgattttta
    58501 tgaactgttt tatgacgacg caaaacgcgc gtcgcaactg ctggatattt cactgaccaa
    58561 acgcggtgct tcggcgggag agccgatccc gatggcgggg attccctacc atgcggtgga
    58621 aaactatctc gccaaactgg tgaatcaggg agagtccgtt gccatctgcg aacaaattgg
    58681 cgatccggcg accagcaaag gtccggttga gcgcaaagtt gtgcgtatcg ttacgccagg
    58741 caccatcagc gatgaagccc tgttacagga gcgtcaggac aacctgctgg cggctatctg
    58801 gcaggacagc aaaggtttcg gctacgcgac gctggatatc agctccgggc gttttcgcct
    58861 gagcgaaccg gccgaccgcg aaacgatggc ggcagagctg caacgcacta atccggcgga
    58921 actgctgtat gcagaagatt tcgccgagat gtcgctgatt gaaggccgtc gcggcctgcg
    58981 ccgtcgcccg ctgtgggagt ttgaaatcga caccgcgcgc cagcagttga atctgcaatt
    59041 tggcacccgc gatctggtcg gttttggcgt cgagaacgcg ccgcgcggac tttgtgctgc
    59101 cggttgtctg ttgcagtatg cgaaagatac ccaacgcacg accctgccgc atattcgttc
    59161 tatcaccatg gaacgtgagc aggacagcat cattatggat gccgcgacac gtcgtaacct
    59221 ggaaattact cagaacctgg cgggcggtgc ggaaaatacg ctggcttctg tgctcgactg
    59281 caccgtcacg ccgatgggta gtcgtatgct gaaacgctgg ctgcatatgc cagtgcgcga
    59341 tactcgcgtg ttgcttgagc gccagcaaac tattggcgca ttgcaggatt tcaccgccga
    59401 gttgcagccg gtactgcgtc aggtcggcga cctggaacgt attctggcac gtctggcttt
    59461 acgaactgct cgcccacgcg atctggcccg tatgcgccac gctttccagc aactgccgga
    59521 gctgcgtgcg cagttagaaa ctgtcgatag tgcaccggta caggcgctac gtgagaagat
    59581 gggagagttt gccgagctgc gcgatctgct ggagcgagca atcatcgaca caccgccggt
    59641 gctggtacgc gacggtggtg ttatcgcatc gggctataac gaagagctgg atgagtggcg
    59701 cgcgctggct gacggcgcga ccgattatct ggagcgtctg gaagtccgcg agcgtgaacg
    59761 taccggcctg gacacgctaa aagttggctt taatgcggtg cacggctact acattcaaat
    59821 cagccgtggg caaagccatc tggcacccat caactacatg cgtcgccaga cgctgaaaaa
    59881 cgccgagcgc tacatcattc cagagctaaa agagtacgaa gataaagttc tcacctcaaa
    59941 aggcaaagca ctggcactgg aaaaacagct ttatgaagag ctgttcgacc tgctgttgcc
    60001 gcatctggaa gcgttgcaac agagcgcgag cgcgctggcg gaactcgacg tgctggttaa
    60061 cctggcggaa cgggcctata ccctgaacta cacctgcccg accttcattg ataaaccggg
    60121 cattcgcatt accgaaggtc gccatccggt agttgaacaa gtactgaatg agccatttat
    60181 cgctaacccg ctgaatctgt cgccgcagcg ccgtatgttg attattaccg gcccgaacat
    60241 gggcggtaaa agtacctata tgcgccagac cgcactgatt gcgctgatgg cctacatcgg
    60301 cagctatgta ccggcacaaa aagtcgagat tggacctatc gatcgcatct ttacccgcgt
    60361 aggcgcggca gatgacctgg cttccgggcg ctcaaccttt atggtggaga tgactgaaac
    60421 cgccaatatt ttacataacg ccaccgaata cagtctggtg ttaatggatg agatcgggcg
    60481 tggaacgtcc acctacgatg gtctgtcgct ggcgtgggcg tgcgcagaaa atctggcgaa
    60541 taagattaag gcattgacgc tgtttgccac ccactatttc gagctgaccc agctaccgga
    60601 gaaaatggaa ggcgtcgcca acgtacatct cgatgcgctg gagcacggcg acactatcgc
    60661 ctttatgcac agcgtgcagg acggcgcggc gagcaaaagc tacggtctgg cggttgctgc
    60721 tctggcgggc gtaccgaaag aggttattaa gcgcgcacgg caaaaactgc gtgagctgga
    60781 aagcatttcg ccgaacgccg ccgcgacgca agtggatggt acgcagatgt ctttgctgtc
    60841 agtgccagaa gaaacttcgc ctgcggtcga agcactggaa aatctcgacc cggattcact
    60901 gactccgcgt caggcgctgg agtggattta tcgcttgaag agtctggtgt aataacaatt
    60961 cccgatagtc ttttgctatc gggaatatta acgacaactg acgaatcaaa taaaaatacc
    61021 ctgtataata ggaaagctta ttttacaggg taaaaccatg ccatctacac gctatcaaaa
    61081 aatcaatgcc catcactatc gccatatatg ggtcgttggt gatattcatg gtgaatatca
    61141 gttattacaa tcccgcttac atcaactctc ttttttcccc gaaaccgact tacttatttc
    61201 tgtcggcgat aatattgatc gtgggccgga gagtcttgac gtcctgcgcc tgctaaacca
    61261 gccctggttt atctccgtta aaggcaacca cgaagcaatg gcgctggatg cgttcgcgac
    61321 cggcgatggc aatatgtggc ttgccagcgg tggtgactag tttttcgatt taaatgattg
    61381 caacaagagg caatagatct gttgctgaaa ttccatcacc ttccacatat tattgaaatc
    61441 actaacgaca taataaaata tgtcatcgca catgcagatt atccgggtga tgaatatctc
    61501 tttggcaaag aaatagcgga gagcgaatta ctctggcctg tttatcgtgt gcagaaatcg
    61561 cttaatggcg agttacaaca aataaacggc gctgattatt ttatatttgg acatatgatg
    61621 tttgataaca ttcagacgtt cgctaaccag atttatattg ataccggatc gccgaaaagt
    61681 gggcggctgt cattttataa aataaggtag tctcatgctt cttctgtgaa gcatgagtaa
    61741 ccagatgtta ttgcaggcca ttattcattt ttcgctacca gcaaagagag atcctgcttc
    61801 gccagcgcgc gactggcact ctccggcaaa ccgtcgtctg taataatctg atcaaactcg
    61861 cttaatggta acgccagcca tgtcgccacc tgaccatatt tcgtcgcatc acagaccaaa
    61921 actcgctggc ggctagcact ggcaatcgcc cgtttcaccg tgactttatc ttccgctggc
    61981 gtagaaatcc cccgcacact ccatgacgat gcagaaataa aagcctgatc aatcatcagg
    62041 ctgcgcagca tggtcgcagc ggcttccccg acacaggaac ggttttcccg acacactgca
    62101 ccgccagtgt gaataattgt gcaattactg ttgtcgagca agtagttcgc aataacgaaa
    62161 tcgtttgtga ccacagtcag tgactccatg tgaatcagat gctgtgctat cgctaacgtg
    62221 gtcgttcccg catccagata gatacaactt cccggctgaa caagacttgc cgccagcttg
    62281 ccaatagccg ctttttgcgt cattgccagc gcagttttta cctgatgaga aggttcatgc
    62341 gccacgcgtc ccggagactg gacgcctccg gacaccagca caacggctcc ctgctgctcc
    62401 agtttttgta aatcccgacg aatggtcata tgtgacacat taattctgtc cgttagttca
    62461 gcaatactga caatgccttt ttcagctacc atctcaagga tgatttggcg acgctctacg
    62521 ggtatcaact tttgctcctt cctttgtcct gctgacattc tacgctattt gcctgcgaaa
    62581 cgtgcgcggc gcaactaacg cttagttcac ataaaataac acaaaatgtt aatttatgtg
    62641 aatcagctca ccatgtcgtt atctcccagc gcttatattc acaatgtcaa acaaaatatc
    62701 acttaaatta acaaggagag cagatgaaaa cgggatctga gtttcatgtc ggtatcgttg
    62761 gcttagggtc aatgggaatg ggagcagcac tgtcatgtgt ccgcgcaggt ttttctacct
    62821 ggggcgcaga cctgaacagc aatgcctgcg ctacgttgaa agaggcaggt gcttgcgggg
    62881 tttctgataa cgccgcgacg tttgccgaaa aactggacgc tctgctggtg ctggtggtca
    62941 atgcggccca ggttaaacag gtgctgtttg gtgaaacagg cgttgcacaa catctgaaac
    63001 ccggtacggc agtaatggtt tcttccacta tcgctagtgc tgatgcgcaa gaaattgcta
    63061 ccgctctggc tggattcgat ctggaaatgc tggatgcgcc agtttctggt ggtgcagtaa
    63121 aagccgctaa cggtgaaatg actgtcatgg cctccggtag cgatattgcc tttgaacgac
    63181 tggcacccgt gctggaagcc gttgccggaa aagtttatcg cataggtgca gaaccgggac
    63241 taggttcgac cgtaaaaatt attcaccagt tgttagcggg cgtacatatt gctgccggag
    63301 ccgaagcgat ggcacttgca gcccgtgcgg ggatcccgct ggatgtgatg tatgacgtcg
    63361 tgaccaatgc cgccggaaat tcctggatgt tcgaaaaccg gatgcgtcat gtggtggatg
    63421 gcgattacac cccgcattca gccgtcgata tttttgttaa ggatcttggt ctggttgccg
    63481 atacagccaa agccctgcac ttcccgctgc cattggcctc aacagcattg aatatgttca
    63541 ccagcgccag taacgcgggt tacgggaaag aagacgatag cgcagttatc aagattttct
    63601 ctggcatcac tctaccggga gcgaaatcat gatcaagatt ggcgttatcg ccgatgattt
    63661 taccggcgcg acggatatcg ccagttttct ggtggaaaac ggtctaccaa cggtacaaat
    63721 taacggtgtt ccaacaggta aaatgccgga agcaatcgac gcactggtga tcagcctgaa
    63781 aacgcgctcc tgtccggtgg ttgaagccac acagcaatcg ctggcggctc tgagctggtt
    63841 gcaacagcaa ggttgcaaac agatctattt caaatacagc tctactttcg acagtacggc
    63901 gaaaggtaat attggcccgg ttaccgatgc attaatggat gctctcgaca cgccgtttac
    63961 ggtcttctct ccggccctgc cggtcaacgg acgtacggtt tatcaggggt attttttcgt
    64021 aatgaatcaa ctgctggccg aatccgggat gcgccatcac ccggtaaatc ccatgaccaa
    64081 cagctatctt ccccgtctgg ttgaagcgca atccacaggg cgctgcggcg tcgtttcggc
    64141 acatgttttc gaacaaggtg tggatgccgt tcgtcaagag ctggctcgct tacagcaaga
    64201 ggactaccgc tacgcggtgc ttgatgcgct gaccgaacac catctggaaa ttcagggaga
    64261 agccttgcgc gatgccccac tggtaacggg cggttctggt ctggcgattg gcctggcccg
    64321 gcagtgggca caagaaaacg gtaaccaggc tcgcgaagca gggcgtccgc tcgctgggcg
    64381 cggcgtagtg ctctccggtt catgctctca aatgaccaac cgctaggtgg cacattaccg
    64441 tcaaattgca ccagcccgtg aagttgatgt ggcacgctgc ctctcaaccg aaactctggc
    64501 cgcttatgca cacgaactgg cagagtgggt tctgggccag gaaagtgtac ttgctccact
    64561 ggtttttgcc accgccagca ctgacgcatt ggcagcaatt caacagcaat acggtgcaca
    64621 aaaagccagt caggcagtag aaactctgtt ttctcaacta gcggcgcggt tagcagcgga
    64681 aggcatgaca cgctttattg tcgcaggtgg tgagacctcc ggcgtagtca cacagagcct
    64741 gggcataaaa gggtttcata ttggcccaac catttccccc ggcgtgccgt gggtaaacgc
    64801 actggataag cctgtctcac tcgcccttaa atctggcaac ttcggtgatg aagccttttt
    64861 ttcacgagcc caaagagagt ttttatcatg agcgatttcg caaaagtaga gcagtctttg
    64921 cgagaggaga tgacgcggat tgccagttca ttctttcagc gcggctatgc aaccggttcg
    64981 gctggcaatc tgtcgctgct tttacctgac gggaatttac tggcgacacc gacaggttca
    65041 tgcctgggca atctcgatcc gcagcggctt tccaaagtcg ccgcggatgg cgaatggtta
    65101 agtggtgaca aaccctcgaa agaggtgctc tttcatctgg cgctgtatcg caacaatccg
    65161 cgctgtaaag cggtggtgca tttgcacagc acatggtcga cggcgctttc ctgcctgcaa
    65221 gggctggaca gcagcaacgt tattcgtccg ttcacaccat acgtggtgat gcggatggga
    65281 aatgtcccgc tggtgcccta ttaccgaccg ggcgataaac gcatcgcaca ggatctggcg
    65341 gaactggcag cagacaatca ggctttttta ctggcaaatc atggcccagt ggtttgcggt
    65401 gaaagcctgc aagaagccgc caacaatatg gaagagctgg aggaaacggc aaagctgatt
    65461 tttattctcg gtgaccgccc gatccgttat ctgaccgcag gt
//
LOCUS       DAETPZ010000010        65471 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.010, whole genome shotgun sequence.
ACCESSION   DAETPZ010000010 DAETPZ010000000
VERSION     DAETPZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.010"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(127..606)
                     /gene="folK"
                     /locus_tag="K0W59_000750"
     CDS             complement(127..606)
                     /gene="folK"
                     /locus_tag="K0W59_000750"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBI3021899.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(603..2021)
                     /gene="pcnB"
                     /locus_tag="K0W59_000751"
     CDS             complement(603..2021)
                     /gene="pcnB"
                     /locus_tag="K0W59_000751"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HBI3021900.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2060..2986)
                     /gene="gluQRS"
                     /locus_tag="K0W59_000752"
     CDS             complement(2060..2986)
                     /gene="gluQRS"
                     /locus_tag="K0W59_000752"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HBI3021901.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQGR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3023..3478)
                     /gene="dksA"
                     /locus_tag="K0W59_000753"
     CDS             complement(3023..3478)
                     /gene="dksA"
                     /locus_tag="K0W59_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HBI3021902.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3656..4360)
                     /gene="sfsA"
                     /locus_tag="K0W59_000754"
     CDS             complement(3656..4360)
                     /gene="sfsA"
                     /locus_tag="K0W59_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBI3021903.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4375..4905)
                     /gene="thpR"
                     /locus_tag="K0W59_000755"
     CDS             complement(4375..4905)
                     /gene="thpR"
                     /locus_tag="K0W59_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HBI3021904.1"
                     /translation="MSEPQRLFFAIDLPAETREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4979..7408
                     /gene="hrpB"
                     /locus_tag="K0W59_000756"
     CDS             4979..7408
                     /gene="hrpB"
                     /locus_tag="K0W59_000756"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HBI3021905.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAQLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPNDPAN
                     TAPTRRTKKYS"
     gene            7604..10138
                     /gene="mrcB"
                     /locus_tag="K0W59_000757"
     CDS             7604..10138
                     /gene="mrcB"
                     /locus_tag="K0W59_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HBI3021906.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10358..12601
                     /gene="fhuA"
                     /locus_tag="K0W59_000758"
     CDS             10358..12601
                     /gene="fhuA"
                     /locus_tag="K0W59_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HBI3021907.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12652..13449
                     /gene="fhuC"
                     /locus_tag="K0W59_000759"
     CDS             12652..13449
                     /gene="fhuC"
                     /locus_tag="K0W59_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HBI3021908.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13449..14339
                     /gene="fhuD"
                     /locus_tag="K0W59_000760"
     CDS             13449..14339
                     /gene="fhuD"
                     /locus_tag="K0W59_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HBI3021909.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14336..16318
                     /gene="fhuB"
                     /locus_tag="K0W59_000761"
     CDS             14336..16318
                     /gene="fhuB"
                     /locus_tag="K0W59_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HBI3021910.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVMLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLIMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16353..17633)
                     /gene="hemL"
                     /locus_tag="K0W59_000762"
     CDS             complement(16353..17633)
                     /gene="hemL"
                     /locus_tag="K0W59_000762"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HBI3021911.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17858..19279
                     /gene="clcA"
                     /locus_tag="K0W59_000763"
     CDS             17858..19279
                     /gene="clcA"
                     /locus_tag="K0W59_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HBI3021912.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LIGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19303..19368
                     /locus_tag="K0W59_000764"
     CDS             19303..19368
                     /locus_tag="K0W59_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3021913.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19361..19705
                     /gene="erpA"
                     /locus_tag="K0W59_000765"
     CDS             19361..19705
                     /gene="erpA"
                     /locus_tag="K0W59_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HBI3021914.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19752..20375)
                     /locus_tag="K0W59_000766"
     CDS             complement(19752..20375)
                     /locus_tag="K0W59_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HBI3021915.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREISMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20413..21213)
                     /gene="btuF"
                     /locus_tag="K0W59_000767"
     CDS             complement(20413..21213)
                     /gene="btuF"
                     /locus_tag="K0W59_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HBI3021916.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21206..21904)
                     /gene="mtnN"
                     /locus_tag="K0W59_000768"
     CDS             complement(21206..21904)
                     /gene="mtnN"
                     /locus_tag="K0W59_000768"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBI3021917.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPTGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21988..23505
                     /gene="dgt"
                     /locus_tag="K0W59_000769"
     CDS             21988..23505
                     /gene="dgt"
                     /locus_tag="K0W59_000769"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HBI3021918.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23635..25059
                     /gene="degP"
                     /locus_tag="K0W59_000770"
     CDS             23635..25059
                     /gene="degP"
                     /locus_tag="K0W59_000770"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HBI3021919.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25214..26370
                     /gene="cdaR"
                     /locus_tag="K0W59_000771"
                     /pseudo
     CDS             25214..26370
                     /gene="cdaR"
                     /locus_tag="K0W59_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26459..26845)
                     /locus_tag="K0W59_000772"
     CDS             complement(26459..26845)
                     /locus_tag="K0W59_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HBI3021920.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27159..27983)
                     /gene="dapD"
                     /locus_tag="K0W59_000773"
     CDS             complement(27159..27983)
                     /gene="dapD"
                     /locus_tag="K0W59_000773"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HBI3021921.1"
                     /translation="MQQLQNIIETAFERRADITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAIIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28014..30686)
                     /gene="glnD"
                     /locus_tag="K0W59_000774"
     CDS             complement(28014..30686)
                     /gene="glnD"
                     /locus_tag="K0W59_000774"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HBI3021922.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWHIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30748..31542)
                     /gene="map"
                     /locus_tag="K0W59_000775"
     CDS             complement(30748..31542)
                     /gene="map"
                     /locus_tag="K0W59_000775"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBI3021923.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31910..32635
                     /gene="rpsB"
                     /locus_tag="K0W59_000776"
     CDS             31910..32635
                     /gene="rpsB"
                     /locus_tag="K0W59_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBI3021924.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32893..33744
                     /gene="tsf"
                     /locus_tag="K0W59_000777"
     CDS             32893..33744
                     /gene="tsf"
                     /locus_tag="K0W59_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBI3021925.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33891..34616
                     /gene="pyrH"
                     /locus_tag="K0W59_000778"
     CDS             33891..34616
                     /gene="pyrH"
                     /locus_tag="K0W59_000778"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBI3021926.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34908..35465
                     /gene="frr"
                     /locus_tag="K0W59_000779"
     CDS             34908..35465
                     /gene="frr"
                     /locus_tag="K0W59_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBI3021927.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35557..36753
                     /gene="ispC"
                     /locus_tag="K0W59_000780"
     CDS             35557..36753
                     /gene="ispC"
                     /locus_tag="K0W59_000780"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBI3021928.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36942..37700
                     /gene="ispU"
                     /locus_tag="K0W59_000781"
                     /gene_synonym="uppS"
     CDS             36942..37700
                     /gene="ispU"
                     /locus_tag="K0W59_000781"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HBI3021929.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37713..38570
                     /gene="cdsA"
                     /locus_tag="K0W59_000782"
     CDS             37713..38570
                     /gene="cdsA"
                     /locus_tag="K0W59_000782"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBI3021930.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38582..39934
                     /gene="rseP"
                     /locus_tag="K0W59_000783"
     CDS             38582..39934
                     /gene="rseP"
                     /locus_tag="K0W59_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HBI3021931.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRVAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39964..42396
                     /gene="bamA"
                     /locus_tag="K0W59_000784"
     CDS             39964..42396
                     /gene="bamA"
                     /locus_tag="K0W59_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HBI3021932.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFREGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42518..43003
                     /gene="skp"
                     /locus_tag="K0W59_000785"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42518..43003
                     /gene="skp"
                     /locus_tag="K0W59_000785"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HBI3021933.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43007..44032
                     /gene="lpxD"
                     /locus_tag="K0W59_000786"
     CDS             43007..44032
                     /gene="lpxD"
                     /locus_tag="K0W59_000786"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HBI3021934.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44137..44592
                     /gene="fabZ"
                     /locus_tag="K0W59_000787"
     CDS             44137..44592
                     /gene="fabZ"
                     /locus_tag="K0W59_000787"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBI3021935.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44596..45384
                     /gene="lpxA"
                     /locus_tag="K0W59_000788"
     CDS             44596..45384
                     /gene="lpxA"
                     /locus_tag="K0W59_000788"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HBI3021936.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45384..46532
                     /gene="lpxB"
                     /locus_tag="K0W59_000789"
     CDS             45384..46532
                     /gene="lpxB"
                     /locus_tag="K0W59_000789"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HBI3021937.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46529..47125
                     /gene="rnhB"
                     /locus_tag="K0W59_000790"
     CDS             46529..47125
                     /gene="rnhB"
                     /locus_tag="K0W59_000790"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBI3021938.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47162..50644
                     /gene="dnaE"
                     /locus_tag="K0W59_000791"
     CDS             47162..50644
                     /gene="dnaE"
                     /locus_tag="K0W59_000791"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBI3021939.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50657..51616
                     /gene="accA"
                     /locus_tag="K0W59_000792"
     CDS             50657..51616
                     /gene="accA"
                     /locus_tag="K0W59_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBI3021940.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51715..53856
                     /gene="ldcC"
                     /locus_tag="K0W59_000793"
     CDS             51715..53856
                     /gene="ldcC"
                     /locus_tag="K0W59_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HBI3021941.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53913..54302
                     /locus_tag="K0W59_000794"
     CDS             53913..54302
                     /locus_tag="K0W59_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBI3021942.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54367..55665
                     /gene="tilS"
                     /locus_tag="K0W59_000795"
     CDS             54367..55665
                     /gene="tilS"
                     /locus_tag="K0W59_000795"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBI3021943.1"
                     /translation="MTLTLNRPLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55714..55974)
                     /gene="rof"
                     /locus_tag="K0W59_000796"
     CDS             complement(55714..55974)
                     /gene="rof"
                     /locus_tag="K0W59_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HBI3021944.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55961..56161)
                     /locus_tag="K0W59_000797"
     CDS             complement(55961..56161)
                     /locus_tag="K0W59_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HBI3021945.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56327..56872
                     /locus_tag="K0W59_000798"
     CDS             56327..56872
                     /locus_tag="K0W59_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HBI3021946.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56869..57291
                     /gene="arfB"
                     /locus_tag="K0W59_000799"
     CDS             56869..57291
                     /gene="arfB"
                     /locus_tag="K0W59_000799"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBI3021947.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     APSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57305..58015
                     /gene="nlpE"
                     /locus_tag="K0W59_000800"
                     /gene_synonym="cutF"
     CDS             57305..58015
                     /gene="nlpE"
                     /locus_tag="K0W59_000800"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HBI3021948.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58170..58994)
                     /locus_tag="K0W59_000801"
     CDS             complement(58170..58994)
                     /locus_tag="K0W59_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HBI3021949.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRSIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59047..60765)
                     /gene="proS"
                     /locus_tag="K0W59_000802"
     CDS             complement(59047..60765)
                     /gene="proS"
                     /locus_tag="K0W59_000802"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBI3021950.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60876..61583)
                     /gene="tsaA"
                     /locus_tag="K0W59_000803"
     CDS             complement(60876..61583)
                     /gene="tsaA"
                     /locus_tag="K0W59_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HBI3021951.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61580..61984)
                     /gene="rcsF"
                     /locus_tag="K0W59_000804"
     CDS             complement(61580..61984)
                     /gene="rcsF"
                     /locus_tag="K0W59_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HBI3021952.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62102..62917)
                     /gene="metQ"
                     /locus_tag="K0W59_000805"
     CDS             complement(62102..62917)
                     /gene="metQ"
                     /locus_tag="K0W59_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HBI3021953.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62957..63610)
                     /gene="metI"
                     /locus_tag="K0W59_000806"
     CDS             complement(62957..63610)
                     /gene="metI"
                     /locus_tag="K0W59_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HBI3021954.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63603..64634)
                     /gene="metN"
                     /locus_tag="K0W59_000807"
     CDS             complement(63603..64634)
                     /gene="metN"
                     /locus_tag="K0W59_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HBI3021955.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64822..65397
                     /gene="gmhB"
                     /locus_tag="K0W59_000808"
     CDS             64822..65397
                     /gene="gmhB"
                     /locus_tag="K0W59_000808"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HBI3021956.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15463 a        16220 c        18045 g        15743 t
ORIGIN      
        1 gggattgata tttatctgcg ggaatttata ttatgtttct gcaaaaatgt ttaaactaaa
       61 atttttatta aggaaagaga ggatgtatga ttttcagaaa atatttatat tcgtaatata
      121 aataaattac catttgctta atttgtcaaa tgctcttgta tgtaagactt cacgcaacgt
      181 ctccccatca ggaaacgcca gctccggtgc aatctcaaac agcggccaca gcataaatcc
      241 acgattcttc atatcgtagt gcggaacggt caggcgttcg gtatttatca cttcattacc
      301 aaacagcatg atatcgagat ccagcgtgcg tggtccccag cgttcagctt tacgaacacg
      361 accctgttgc agttctatgc gctgtgtatg gttgagtaac tcttccgggg cgggcgaagt
      421 ttcaagcgcc acggcggcgt tgaggtaatc gggttgatct tgcggcccca gcggtggggt
      481 gcggtaaaac gaagaaacgg caagaatgcg gctttcaggg atatcgccta atgctttcag
      541 ggcagcattg acctgctcca gcggagaggc cagattgctg cctatggcaa tatacgccac
      601 tgtcatgtgg taccctcacg acgcggtgcg cgtttgcgtg gacgacgagt acgacgacgc
      661 ggtgacggtt cttcatccag ctcgttaagc atcccttttt gatctggtgg tgcggaaacc
      721 tggaactcac cccaccattt caccagacgc tgcagttcag cattacgttc aacttcagct
      781 cgcaaggcca acaggtcata agccgcacgg aacttaggat gctccagcag tttccatgcg
      841 cgtttaccct gacgacggga catacgcaac tgcaactgcc agatatcgcg ggttaacgtc
      901 gtcagacgtt tcgggattgc cagtgaacgg caggcttcgt ccagcacgtc gttcatcgcc
      961 agcgcgaaag cgtcgtgata ggtcaggccg ctttcctggg cgatcttctg tgccgtctcc
     1021 agcagtgggt accagaacat ggcggcaaac aagaacgccg ggttcacgcg catatcgtta
     1081 tggatacgcg tatcggtatt cttcagcacc tgttcaatga tccgctccat cgggctgtcg
     1141 ccattttccg tgaagtagcg ggtaatggtc gggaacagcg gctggaacag atgatattca
     1201 cacaacagct tataggtttc gtaaccgtag cccgcttgta gcagtttaag cgattcttca
     1261 aacaggcgtg ccggtgggat atcgttcagc agggtagcga ggcgagggat cggttctgcg
     1321 gtttccgggc tgatgcgcat acccaatttg gcggcaaaac gtaccgcgcg cagcatacgt
     1381 accggatctt cacggtagcg cgtttccggg ttaccaatca gacggataac gccgtccttc
     1441 agatccttca taccgccaac gtaatcacgg acggtaaaat ccgctacgct gtaatacagg
     1501 ctgttgatag tgaaatcgcg gcgctgggcg tcttcttcga tggagccgaa aatgttgtcg
     1561 cgcagcaaca tgccgttttg cccgcgttgg gaggtcgtgc ggtcgctgac gttaccttcg
     1621 tggtgtccac ggaaggtcgc aacttcgata atctccgggc caaacattac atgagccaga
     1681 cggaaacggc gacccaccag gcggcagtta cggaacagtt tgcgcacctg ctcaggcgtg
     1741 gcgttagtgg ttacgtcaaa atctttcggc tttttgccaa gtaacaggtc gcgcacgccg
     1801 ccgccaacca gccaggcttc gtatcccgct ttgttgagcc tgtacattac cttcagggca
     1861 ttttcactga tatctttgcg ggaaatagca tgctgctcac gcgggatcac tgtcacctgt
     1921 ggacgggcga ctgcctgttc agcctcgctt tcctcgcggc ttagcacctt gcggcaaaaa
     1981 ttagcgactc gggtaaaaat agtacacctc ggtagtgtca aacatcattc aggacaaaaa
     2041 aatagcggct aatcatagct cagcatgacg catttgagaa tgttgaattt acaattgccg
     2101 actcgggcac ggcggtcagc atccagtttt tgacggctga ctgaaggatt tgctcgacgc
     2161 tgaaatcctg ccagtgtgct tctgcctgct gccccagaaa ttgaagtgcc gcgattagta
     2221 ccgggcgtgg atcgcctttc ggcaacgcag gcgcatgatt ctgcttggaa agtttagcgc
     2281 cttgtggatt aagcgccagc ggcagatgaa tgtaatctgg cactttccag ccaaaaagct
     2341 ggtacagcga gatttgcctt actgttggtt caatcagatc agccccacgc actatttctg
     2401 taacgccctg gaaatgatca tcaaccacaa cagccaggtt gtaggcgaac aacccatcac
     2461 ggcgatgaat gataaaatct tcccgtgcca gtttttcgtc ggcgtgaata atgcctcgca
     2521 gcaggtcagt aaattgcgtg accggatgct gctggcggat acgcactgcg gcgttgtctg
     2581 gtccatgatg caacacccgg caatgaccgt cgtaaatacc gccaatgctt tgaatacgcg
     2641 cacgcgtaca ggtgcagtaa taacttagtc cttgttcatg taaccaggcg agtgcttcac
     2701 gataggcgtc gtgacgttgc gattgccaga gaacatcgcc gtcccagtgc agaccgtaat
     2761 gttccagctg gcgcaggata gtttctgcgg caccgggaac ttcacgaggc gggtcgatat
     2821 cttctatgcg taccagccag cgaccttgcc gggcgcgacc ctgcaaatag ctgccgagcg
     2881 cggcgatcag agagccaaaa tgaagctcgc cggaaggaga gggggcgaag cggccaatat
     2941 actgtgtgtc tgtcatctct ttgaacaaaa aataaggcgg gagcatttcc cgcctgtggt
     3001 aaacgtgatg gaacggctgt aattagccag ccatctgttt ttcgcgaatt tcagccagcg
     3061 ttttgcagtc gatgcacaga tcggctgtcg ggcgcgcttc cagacggcga ataccaattt
     3121 caacaccgca ggattcgcag tagccgaaat cttcgtcttc cacttttttc agcgtcttct
     3181 cgatcttttt gatcagctta cgctcgcgat cgcggttacg cagttcgagg ctgaactctt
     3241 cttcctgggc tgcacggtct accgggtccg ggaagttggc tgcttcatcc tgcatatgtg
     3301 taacggtgcg atcgacttca tccctgagtt gattacgcca tgcttccaga atacgacgga
     3361 agtgcgccag ctgggcttca ttcatatact cttcgcccgg cttctcctga tatggttcca
     3421 ccccagcgat ggcgagaata ctcagggacg atgttttacg gttttgccct tcttgcatgt
     3481 tgcttctcct taacacgcac tatcgatccc catgttcggg ggaaaaatga ggccgctata
     3541 aatagcagat gcttttccgg atagcaatta tctaaacgta acacttgaca actgtgtgag
     3601 gaaaagcgta ttcgcgcacg cgaccagaat gtaaattaac cagttactta ctttactaca
     3661 atgtaaccgg cagtgatttt ttaagagcca tgccttcagc agaaatttcc gctttgtaag
     3721 ccagaatttc tacccccctc tgttgagctt ctgacaatag ttgcgcgtat ttctcatcga
     3781 tgtggcgcgc gggtgaaaac cgtgtaatgg ctgaatgcag cacggcgaaa aagataaccg
     3841 cacgctggcc ttcagccgct acgctcatca actcccgaag gtgtttctga cctcgttcag
     3901 tgaccgcatc gggaaaatat ccctgttcgt tctccgctaa cgtaaccgat ttcacttcaa
     3961 tatagcagtc tggacgcgaa tccgcctgca acataaagtc aatacggctg cgttcggagc
     4021 cgtattttac ttcgcttttc agcgagctat agcctgacag ttctgaaatt gattcattaa
     4081 ggatagcctc tttcgtcaac ctgttagccc aaagcgtatt gacgcaaata aatgcgccgc
     4141 tctggctttg agttaattcc caggtgtgtg ggtatttccg tttggtgttg tctgaagtcg
     4201 aataccagac ggtatcgcca ggcgttgcac aaccggtcat cgcacccgta ttcgggcagt
     4261 gtagcgttaa ttcgcgacca tcgggtgtga tcacatcggc taaaaaacgt ttgtaacgct
     4321 gaattagcgt cgcgcgctgt agagggggag aaaattccat tgcgacaatc cttgttattg
     4381 cgttagcgcc cagcgtttta gcggcgtgta gcgtgtgcgt ccacgggcaa acgaggaggc
     4441 gtaaagggtg aactccgtca ccgcataaga ccagttaaaa cccggcggcg ggaatgtcac
     4501 cgcctcgctg gcgtcgcgca ataaggtaat atgtggatga aacggacgat tgctttgaaa
     4561 acaaccgctg cgggcagcct gtgaacggag catattcgcc agctggatta agccgcgtgg
     4621 aggctgacgc atccctaacc acaccacacg cgaacgcagc cattgtccgg cgtcatcaag
     4681 cgtgagtgtg aaacctggtt gacgaatccg tccggctaaa agagaaagcg ccttctcttt
     4741 ctctgcgctc acttcgccta aaaatgccag agtcagatgc aaattatcgg cggcgaccgg
     4801 acgtcccgcc tcaggtggga agtgtgtggc gcgccaatgg ataatctgtt cgcgggtttc
     4861 tgcaggtaag tcgatagcaa agaacagacg ttgcggttca gacatgtgag gcactcggtt
     4921 atgaattacc gcgatgctac aatgtggcgc gaagaatgtt aaccctctgg agcgttttgt
     4981 gtcgtcgttg cccgttgctg ccgtcttacc tgaattactt accgctctcg attgtgcgcc
     5041 gcaggtatta ttaagtgcgc cgaccggggc cgggaaatca acctggctgc cgctgcaact
     5101 gctggcgcat cccggcatta acgggaaaat tatcctgctg gagccgcgtc gtctggcggc
     5161 gcgtaacgtc gcgcaacggc tggcggagct gcttaacgaa aagccaggcg ataccgttgg
     5221 ctaccggatg cgtgcgcaaa actgcgtcgg gccgaatacc cgcctggaag tggttaccga
     5281 aggcgtgctg acgcggatga tccagcgtga cccggaactg agcggtgttg gattggtgat
     5341 ccttgatgag tttcatgagc gcagtttgca ggcggatttg gcgttggcgc tgttactcga
     5401 tgtgcaacaa ggtctgcgtg atgaccttaa actgctgatt atgtcggcta cgctggacaa
     5461 tgaccgcttg cagcaaatgc tgccggaagc gcccgtcgtc atctcagaag ggcgctcgtt
     5521 tccggttgaa cgccgttatt taccgctgcc cgcgcatcag cgttttgacg aagccgttgc
     5581 ggtagccacc gccgaaatgg tgcgtcagga aagtggatca ttactgttat ttttacctgg
     5641 cgtcggagaa attcagcgcg tgcaggaaca actggcttcg cgcatcggca gtgatgtggt
     5701 gctctgcccg ctgtatggcg cgttgtcact gaacgatcag cgaaaagcga tcctcccggc
     5761 accgcaaggg atgcgcaaag tggtgctggc gaccaatatt gctgaaacca gtttaaccat
     5821 cgaaggtatt cgtctggtgg tggattgtgc ccaggagcgt gtggcgcgct ttgatccgcg
     5881 cacggggctt acgcggttga ttactcaacg cgttagtcag gcatccatga cgcagcgtgc
     5941 cgggcgcgcc gggcgtctgg agccgggtat cagcctgcat ttaatcgcca aagaacaagc
     6001 agaacgcgcc gcggcgcaaa gtgagccgga gatcttacaa agcgatcttt ccggtttgct
     6061 gatggaatta ctgcaatggg gatgcagcga tccggcgcag atgagctggc tggatcaacc
     6121 gccaacggtg aatctactgg ccgcgaaacg cctgttacaa atgctggggg cgctggaggg
     6181 tgaacggctt agtgcgcaag ggcaaaaaat ggcagcgctg ggtaacgatc cgcgtttagc
     6241 ggcaatgctg gttaacgcaa agagcgatga cgaagctgct accgcggcaa aaattgccgc
     6301 cattctcgaa gagccgccac ggatgggcaa tagtgacctg ggcgtggcgt tttcgcgcaa
     6361 tcaaccagcc tggcagcaac gtagtcagca actgttaaaa cgcttaaacg tacgcggcgg
     6421 tgaggcagac agttcgctta tcgcgccgct acttgccggg gcgtttgccg atcgcattgc
     6481 tcatcgccgt gggcaagatg gacgctatca actggcaaac ggcatgggag cgatgctcga
     6541 tgccgacgac gcgctaagcc gccacgaatg gttgatcgca cagttattat tgcagggcag
     6601 cgcctcgccg gatgcgcgga ttttactggc gctaccggtc gatattgatg aattagtaca
     6661 acgctgtccg cagctggtgc agcaatctga caccgtggaa tgggatgacg cgcaaggtac
     6721 gctgaaagcc tggcgtcggc tacaaatcgg tcagttgacg gtgaaagtgc agccgctggc
     6781 gaaaccgtca gaagacgagt tgcatcaggc gatgcttaat ggcatccgtg ataaaggttt
     6841 aagcgtgctc aactggacgg cggaagcgga acagctacgc ttgcgtttgt tatgcgccgc
     6901 aaagtggttg ccggaatatg actggccagc ggttgatgat gaaagtttgt tggcgacgct
     6961 ggaaacgtgg ctgctgccac atatgaccgg cgtacattca ctacgcggcc tgaaatcact
     7021 cgacatttat caggcactac gcggattact tgattgggga atgcagcaac gtctggatag
     7081 tgaattgcct gcgcattaca ctgtgccgac gggaagccgg atcgccattc gttatcatga
     7141 agataacccg cccgcgctgg cggtgagaat gcaggagatg tttggtgagg ccactaatcc
     7201 gacgatcgcc caggggcgcg tgccgctggt gctgaagttg ctttcccctg cccaaaggcc
     7261 gctgcaaatc acgcgtgatt tgggggcctt ctggaaaggg gcgtatcgtg aggtgcaaaa
     7321 ggagatgaaa gggcgttatc ccaaacacgt ctggccgaac gacccggcaa acacggcacc
     7381 gacgcgacgg acgaaaaagt attcgtaagg ttggttttct ccctctccct gtgggagagg
     7441 gccggggtga gggcatcagc gcgcacgttc accctcaccc taaccctctc cctcaaggga
     7501 gaggggaccg atcgagcaca aattttgaga gatatcttct tctgtcttgt aacagaagaa
     7561 cagaaaatcg ggcttttgcg cctgaatatt gcggagaaaa agcatggccg ggaatgaccg
     7621 cgagccaatt ggacgcaaag ggaaaccgac gcgtccggtc aaacaaaagg taagccgtcg
     7681 tcgttacgaa gatgacgatg attacgacga ttatgatgac tatgaggatg aagaaccgat
     7741 gccgcgcaaa ggtaagggca aaggcaaagg gcgtaagcct cgtggcaaac gcggctggct
     7801 atggctactg ctaaaactgg ctatcgtttt tgccgtgctg atcgccattt acggcgttta
     7861 tctcgatcaa aaaattcgta gccgtattga tggcaaggtc tggcaactgc ccgcggcagt
     7921 ttatggccga atggtcaacc ttgaaccaga tatgaccatc agcaagaacg aaatggtgaa
     7981 gctgctggag gcaacacagt atcgtcaggt gtcgaaaatg acccgtcctg gcgaatttac
     8041 cgtgcaggcc aacagcattg agatgatccg ccgtccgttt gatttcccgg acagtaaaga
     8101 agggcaggtg cgtgcgcgtc tgacctttga tggtgatcat ctggcgacca tcgtcaatat
     8161 ggagaacaac cgtcagttcg gtttcttccg tcttgatccg cgtctgatca ccatgatctc
     8221 ctcgccaaac ggtgagcagc gtttgtttgt gccgcgcagt ggtttccctg atttgctggt
     8281 ggatactttg ctggcgacag aagaccgtca tttttacgag catgatggaa tcagtctcta
     8341 ctccattggg cgtgcggtgc tggcaaacct gaccgcaggt cgcacggtgc agggtgcgag
     8401 tacgctgacg caacagctgg tgaaaaacct gttcctctcc agcgagcgtt cttactggcg
     8461 taaagcgaac gaagcttaca tggcgctgat catggacgcg cgttacagca aagaccgtat
     8521 tcttgagctg tatatgaacg aggtgtatct cggtcagagc ggcgacaacg aaatccgcgg
     8581 cttcccgctg gcaagcttgt attactttgg tcgcccggta gaagagctaa gcctcgacca
     8641 gcaggcgctg ttagtcggta tggtgaaagg ggcgtccatc tacaacccgt ggcgtaaccc
     8701 gaaactggcg ctggagcgac gtaatctggt gctgcgtctg ctgcaacagc aacagattat
     8761 tgatcaagaa ctctatgaca tgttgagtgc ccgtccgctg ggggttcagc cgcgtggtgg
     8821 ggtgatctct cctcagccag cctttatgca gttggtgcgt caggagttgc aggcaaaact
     8881 gggcgataag gtaaaagatc tctccggcgt gaagatcttc actacctttg actcggtggc
     8941 ccaggacgcg gcagaaaaag ccgccgtgga aggcattccg gcactgaaga aacagcgtaa
     9001 gttgagcgat cttgaaactg cgattgtggt cgtcgaccgc tttagtggtg aagttcgtgc
     9061 gatggtcgga ggttctgagc cgcagtttgc gggctacaac cgtgcgatgc aggcgcgtcg
     9121 ttcgattggt tcccttgcaa aaccagcgac ttatctgacg gctttaagcc agccgaaaat
     9181 ctatcgcctg aacacgtgga ttgccgatgc gccaatcgcg ctgcgtcagc cgaatggcca
     9241 ggtctggtca ccgcagaacg atgaccgtcg ttatagcgaa agcggcagag tgatgctggt
     9301 ggatgcgttg acacgttcga tgaacgtgcc gacggtaaat ctggggatgg cgctggggct
     9361 gcctgcggtt acggagacct ggatcaaact gggcgtaccg aaagatcagt tgcatccggt
     9421 tccggcaatg ctgctggggg cgttgaactt aacgccaatc gaagtggcgc aggcattcca
     9481 gaccatcgcc agcggtggta accgtgcacc gctttctgca ctgcgttcgg taatcgcgga
     9541 agatggtaag gtgctgtatc agagcttccc gcaggcagaa cgcgctgttc cggcgcaggc
     9601 ggcgtatctg acactatgga ccatgcagca ggtggtacaa cgtggtacgg gtcgtcagct
     9661 tggggcgaaa tacccgaacc tgcatctggc agggaaaaca gggactacca acaataacgt
     9721 agatacctgg tttgcaggca ttgacggcag cacggtgacc atcacctggg taggccgtga
     9781 taacaaccag ccgaccaaac tgtatggagc cagcggggca atgtcgattt atcagcgtta
     9841 tctggctaac cagacgccaa cgccgctgaa tcttgttccg ccagaagata ttgcagatat
     9901 gggcgtggac tacgacggca actttgtttg cagcggtggc atgcgtgtct tgccggtctg
     9961 gaccagcgat ccgcaatcgc tgtgccagca gagcgagatg cagcagcagc cgtcaggcaa
    10021 tccgtttgat cagtcttctc agccgcagca acagccgcaa cagcaacctg ctcagcaaga
    10081 gcagaaagac agcgacggtg tagccggttg gatcaaggat atgtttggta gtaattaaca
    10141 tctaagcgtg aaataccgga tggcgagttg ccatccggta aaataacatc ctatctaaga
    10201 tattaaccct ttcttttcat ctggttgttt attaaccctt caggaacgct cagattgcgt
    10261 accgcttgcg aacccgccag cgtttcgaat attatcttat ctttataata atcattctcg
    10321 tttacgttat cattcacttt acatcagaga tataccaatg gcgcgttcca aaactgctca
    10381 gccaaaacac tcactgcgta aaatcgcagt tgtagtagcc acagcggtta gcggcatgtc
    10441 tgtttatgca caggcagcgg ttgaaccgaa agaagacact atcaccgtta ccgctgcacc
    10501 tgcgccgcaa gaaagcgcat gggggcctgc tgcaactatt gcggcgcggc agtcagctac
    10561 cggcactaaa accgatacgc cgattcaaaa agtgccacag tctatttctg ttgtgaccgc
    10621 cgaagagatg gcgctgcatc agccgaagtc ggtaaaagaa gcgcttagct acacgccggg
    10681 tgtctctgtt ggtacgcgtg gcgcatccaa cacctatgac catctgatca ttcgcggttt
    10741 tgcggcagaa ggccaaagcc aaaataacta tctgaatggc ctgaagttgc agggcaactt
    10801 ctataacgat gcggtcattg acccgtatat gctggaacgc gctgaaatta tgcgtggccc
    10861 ggtttccgtg ctttacggta aaagcagtcc tggcggtctg ttgaatatgg tcagcaagcg
    10921 tccgaccacc gaaccgctga aagaagttca gtttaaagcc ggtactgaca gcctgttcca
    10981 gactggtttt gactttagcg atgcgttgga tgatgacggt gtttactctt atcgcctgac
    11041 cggtcttgcg cgttctgcca atgcccagca gaaagggtca gaagagcagc gttatgctat
    11101 tgcaccggcg ttcacctggc gtccggatga taaaaccaat ttcaccttcc tttcttactt
    11161 ccagaacgag ccggaaaccg gttattacgg ctggttgccg aaagagggaa ccgttgagcc
    11221 gctgccgaac ggtaagcgtc tgccgacaga ctttaatgaa ggggcgaaga acaacaccta
    11281 ttctcgtaat gagaagatgg tcggctacag cttcgatcac gaatttaacg acacctttac
    11341 tgtgcgtcag aacctgcgct ttgctgaaaa caaaacctcg caaaacagcg tttatggtta
    11401 cggcgtctgc tccgatccgg cgaatgctta cagcaaacag tgtgcggcat tagcgccagc
    11461 ggataaaggc cattatctgg cacgtaaata cgtcgttgat gatgagaagc tgcaaaactt
    11521 ctccgttgat acccagttgc agagcaagtt tgccactggc gatatcgacc acaccctgct
    11581 gaccggtgtc gactttatgc gtatgcgtaa tgacatcaac gcctggtttg gttacgacga
    11641 ctctgtgcca ctgctcaatc tgtacaatcc ggtgaatccc gatttcgact tcaatgccaa
    11701 agatccggca aactccggcc cttaccgcat tctgaataaa cagaaacaaa cgggcgttta
    11761 tgttcaggat caggcgcagt gggataaagt gctggtcacc ctaggcggtc gttatgactg
    11821 ggcagatcaa gaatctctta accgcgttgc tgggacgacc gataaacgtg atgacaaaca
    11881 gtttacctgg cgtggtggtg ttaactacct gtttgataat ggtgtaacac cttacttcag
    11941 ctatagcgaa tcgtttgaac cttcttcgca agttgggaag gatggtaata ttttcgcacc
    12001 gtctaaaggt aagcagtatg aagtcggcgt gaaatatgta ccggaagatc gtccgattgt
    12061 agttactggt gccgtgtata atctcactaa aaccaacaac ctgatggcgg accctgaggg
    12121 ttccttcttc tcggttgaag gtggcgagat ccgcgcacgt ggcgtagaaa tcgaagcgaa
    12181 agcggcgctg tcggcgagtg ttaacgtagt cggttcttat acttacaccg atgcggaata
    12241 caccaccgat actacctata aaggcaatac gcctgcacag gtgccaaaac acatggcttc
    12301 gctgtgggct gactatacct tctttgacgg tccgctttca ggtttgacgc tgggcaccgg
    12361 tggtcgttat actggctcca gctatggtga tccggctaac tcctttaaag tgggaagtta
    12421 tacggtcgtg gatgcgttag tgcgttatga tctggcgcga gtcggcatgg cgggctccaa
    12481 cgtggcgctg catgttaaca acctgttcga tcgtgaatac gtcgccagct gctttaacac
    12541 ttatggctgc ttctggggcg cagaacgtca ggtcgttgca accgcaacct ttcgtttcta
    12601 atttctcttt tggggcacgg atttccgtgc ccatttcaca agttggctgt tatgcaggaa
    12661 tacacgaatc attccgatac cacttttgca ctgcgtaata tctcctttcg tgtgcccggg
    12721 cgcacgcttt tgcatccgct gtcgttaacc tttcctgccg ggaaagtgac cggtctgatt
    12781 ggtcacaacg gttctggtaa atccactctg ctcaaaatgc ttggtcgtca tcagcctccg
    12841 tcagaagggg agattcttct tgatgcccag ccgctggaaa gctggagcag caaagcgttt
    12901 gcccgcaaag tggcttattt gccgcagcag cttcctccgg cagaagggat gaccgtgcgt
    12961 gaactggtgg cgattggtcg ttacccgtgg catggcgcgc tggggcgctt tggtgcggca
    13021 gatcgcgaaa aggtcgagga agctatctcg ctggttggct taaaaccgct ggcgcatcgg
    13081 ctggtcgata gtctctctgg cggcgaacgt cagcgggcgt ggatcgccat gctggtggcg
    13141 caggatagcc gttgtctgtt gctcgacgaa ccgacttcgg cgctggatat cgcccaccag
    13201 gttgatgtgc tggcgctggt gcaccgttta agtcaggaac gtggcctgac ggtcattgcc
    13261 gtgttgcacg atatcaatat ggcggcacgc tactgtgatt atctggtcgc cctgcgcagt
    13321 ggtgaaatga ttgctcaggg aacgcctgcg gaaattatgc gcggcgaaac cctcgaaatg
    13381 atttatggca tcccgatggg tattttgccg catccggcgg gtgctgcacc tgtgagtttt
    13441 gtttattgat gagcggctta cctcttattt cgcgccgtcg actgttaacg gcgatggcgc
    13501 tttctccgtt gttatggcag atgaataccg cccacgcggc ggctattgac cccaatcgta
    13561 ttgtggcgct ggagtggttg ccggtggaat tactgctggc gctcggcatc gtgccttacg
    13621 gcgtggcgga taccatcaac tatcgcctgt gggtcagcga accaccattg ccggactcag
    13681 tgattgacgt cggtttgcgt acagaaccta accttgaact gctgaccgaa atgaaaccgt
    13741 cgtttatggt ctggtcggca ggatatggcc cttcatcaga aatgctggca cgtattgcgc
    13801 cgggtcgcgg atttaacttc agtgacggca aacatccgtt ggcgatggcg cgtaaatcgc
    13861 tgacggaaat ggcagattta cttaacctgc aaagcgcagc ggaaacgcat ttagcgcaat
    13921 acgaagactt tatccgcagc atgaaacccc gctttgtgaa gcgtggtgca cgcccgttac
    13981 tgctgacgac gcttatcgat ccgcgccata tgctggtctt cggtccaaac agcttgttcc
    14041 aggaaattct cgatgagtac ggcatcccaa atgcctggca aggggaaacc aacttctggg
    14101 gcagtaccgc cgtcagtatc gatcgtctgg cagcgtataa agacgttgat gtgctctgtt
    14161 ttgatcacga caacagcaaa gacatggatg cgctaatggc aaccccgctg tggcaggcca
    14221 tgccgtttgt ccgtacaggg cgctttcagc gcgtacccgc agtctggttt tatggtgcaa
    14281 cgctttcggc aatgcacttt gtgcgtgttc tggataacgc catcggaggt aaagcgtgag
    14341 taaacgaatt gcgcttttcc cggcgttatt gctggcgctg ttagtgattg tcgctacggc
    14401 gctcacctgg atgaacttct cacaggcgct gccgcgcagt cagtgggcgc aggccgcctg
    14461 gtcgccggat attgacgtca tcgagcagat gatttttcac tacagcttgt tgccgcgtct
    14521 ggcgatttcg ctgctggtgg gcgcgggcct ggggctggtg ggcgtgctgt ttcagcaagt
    14581 actgcgtaac ccgctggcgg aaccgacgac gttgggcgtt gcaacaggcg cgcaactggg
    14641 gattaccgtc accacgctct gggcgatccc tagcgcgatg gcgagccagt ttgctgcgct
    14701 ggcaggggct tgcgttgttg gcttaattgt ctttggcgtc gcgtggggga aacggctgtc
    14761 gccggtaacg ctgattctcg cggggttggt agtgagcctt tattgcggcg caatcaatca
    14821 gttactggtt atcttccatc atgaccagtt gcaaagcatg tttctgtgga gcactggaac
    14881 gctgacgcaa accgactggg gcggtgttga gcgtttatgg ccgcagttgc tgggcggtgt
    14941 gatgctgacg ttgctgctac ttcgtccgtt aaccctgatg gggcttgatg atggcgtggc
    15001 gcgcaatctc gggctggcct tgtcgcttgc gcgtctggcg gcgctgtcgc tggcgattgt
    15061 catcagtgcg ctgctggtga acgctgtggg gattatcggc tttatcgggt tgttcgcgcc
    15121 gctgctggca aaaatgctgg gggcgcggcg tctgctgcca cgactgatgc tggcgtcgtt
    15181 gattggtgcg ctgatcctct ggctttccga tcaaatcatc ctctggctga ctcgcgtgtg
    15241 gatggaagtg tccaccggtt cggtcactgc gttgatcggt gcgccgctgc tactgtggct
    15301 gctgccgcgt ttacgcagca ttagcgcgcc ggatatgaag gttaacgatc gtgtcgcgac
    15361 tgaacgccaa catgtgctgg cgtttgccct cgcgggcggc gtgctgttgt tgatggctgt
    15421 ggtggtggcg ctgtcgtttg gtcgtgatgc gcacggctgg acgtgggcga gcggggcgtt
    15481 gctcgaggat ttaatgccct ggcgctggcc gcgaattatg gcggcgctgt ttgcgggcgt
    15541 catgctggcg gtagcgggct gtattattca gcgactgacc ggcaacccga tggcaagccc
    15601 ggaagtgctg gggattagct ccggcgcggc gtttggtgtg gtgttaatgc tgtttctggt
    15661 accgggtaat gcctttggct ggttgttacc cgcaggcagt ctcggggcgg cggtgatgct
    15721 gttgatcatt atgatcgccg ccggacgcgg tggattttcc ccacaccgca tgttactggc
    15781 ggggatggcg ttaagtaccg cgttcaccat gcttttgata atgttgcagg caagtggtga
    15841 cccgcgaatg gcgcaagtgc tgacctggat ttccggttcg acctacaacg cgaccgatgc
    15901 tcaggtctgg cgcaccggaa ttgtgatggt gattttgctg gcgattactc cgctgtgccg
    15961 ccgctggctg accattttac cgctgggtgg tgataccgcg cgggcggtag ggatggcgct
    16021 gacgccgacg cgaattgcgc tgctgctgtt agcggcttgc ctgacggcga ccgcgacgat
    16081 gactattgga ccgttgagtt ttgttggttt aatggcaccg catattgcgc ggatgatggg
    16141 ctttcgacgg acgatgccac atatcgtaat ttcggcgctg gtgggtggtt tactgctggt
    16201 gttcgctgac tggtgtgggc ggatggtgct gtttccattc cagatcccgg cggggctgct
    16261 gtcgaccttt atcggtgcgc catattttat ctatttgctg agaaagcaga gccgttaata
    16321 gaatgcctcg cccggtgggc gaggcaaaat tatcacaact tcgcgaacac ccgacgtgca
    16381 gcatcgatgg tgttattgat atcttccatg ctgtgcgcca cagacataaa gcccgcttca
    16441 aacgctgacg gtgccaggta aacaccttca tccagcatca tatggaagaa acgcttaaaa
    16501 cgctcgacgt cacaggccat cacatcctga tagcacgtca cggactcggc gtcggtaaag
    16561 aagataccga acataccacc gacgtggtta acgaccagcg gaattcccgc ttcttctgcc
    16621 gcttccagca gaccttctgc cagacgtgtt gtcagctcat ccagcgtttc gtgaatgccc
    16681 ggctgcgcga cttcattcag acaggcgaaa cccgctgcca tcgcaatcgg gttaccggaa
    16741 agcgtacccg cctgatagac cggacctgtc ggggccagcg catccattac atcacgacga
    16801 ccaccgaatg cgcctaccgg cattccaccg ccgatgattt ttccgaggca ggttaaatct
    16861 ggcactacgc cgaaataatc ctgtgcgcca gctagcgcta cgcggaaacc ggtcatcact
    16921 tcatcgatga tcagcaacgc gccaaattcg tcgcacagcg cacgcagacc tggcaggaac
    16981 tctggcagcg gcggaacaca gttcatattg cctgccaccg gctcgacgat aatacaggca
    17041 atctcttgcg ggtattgctc aaacgcggcg cgtacagagg ccagatcgtt ataagtacag
    17101 gttaaggtat gtttggcgaa atctgccgga acgcccggcg agtttggctg gcctaacgtg
    17161 agtgcgccag aaccggcttt caccagcagg cagtcagcgt gaccgtggta acaaccttca
    17221 aatttaatga ttttgtcgcg accggtaaaa ccacgggcca ggcggatggc gctcatcgtc
    17281 gcctcggtgc cggagttcac catgcgcacc atatccatgg tcgggaccag ttcggtcacc
    17341 agttgcgcca ttttcacttc catttcggtc ggtgcaccaa agcttaaacc acgctcggcg
    17401 gcttcaatca cggcattgcg gatcgccgga tggttatggc ccagcaccat cggcccccag
    17461 gaaccgacat aatcgatata ggctttgcca tcaacatcgt acagataagc gccgtccgct
    17521 ttttcgataa acagtggagt gccgcccacg ccagtaaagg cgcgaacagg ggagttcaca
    17581 ccgccaggga tcagctcgcg cgctgcgctg taaagatttt cagacttact catggagggt
    17641 tcctgattcg tagaaaaagt gaatggctgc tattctatgt tattcataac aagttaaata
    17701 ctcgtcaaac atcaggctgc ttgtactggt gcaatcctga tttcttagag tataaaagtt
    17761 ttgtgcattt gaaacattac gctttgcaaa ggattttcat ggaacgtgcg agtaaaatgc
    17821 cgtcatctta tttgtatgac caataagtga tcattggatg aaaactgata ctccctcttt
    17881 agaaacaccg caggccgcgc gcctgcgacg cagacaactg attcgccaac ttcttgagcg
    17941 cgataaaacc ccgttagcca ttttgtttat ggcggcagtc gtcggcacgc ttatcgggct
    18001 ggctgcggtt gcttttgaca aaggtgtcgc ctggttgcag aaccaacgta tgggggcgct
    18061 ggtacatact gctgataatt atccgctact gttaaccgtc gcttttctct gttcggcggt
    18121 gctggcgatg ttcggctact ttttggtgcg caaatacgcg cccgaagcag ggggatcggg
    18181 gatcccggaa attgaagggg cgctggaaga tcaacgtcct gttcgctggt ggcgtgtatt
    18241 gccggtgaaa ttctttggcg ggctggggac actcggcgga ggcatggtgc tagggcgcga
    18301 aggaccaacc gtgcagatcg gcggcaatat cggtcgcatg gtgcttgata ttttccgcct
    18361 gaaaggtgat gaagctcgcc atacgctgct ggcaaccggt gctgctgcgg ggctggctgc
    18421 ggcctttaac gcgccgctgg cgggtatttt gtttattatc gaagagatgc gtccgcagtt
    18481 tcgctatacg ttaatttcga ttaaagcggt atttattggc gtcattatgt cgaccattat
    18541 gtaccggatt tttaatcatg aagttgcgtt gattgacgtc ggtaaacttt ctgatgcgcc
    18601 gcttaatacg ctgtggcttt atctgatcct cggtattatt tttggcattt tcggccctat
    18661 ttttaataaa tgggtgctgg ggatgcagga tttgctgcac cgtgtgcacg gcggcaatat
    18721 taccaaatgg gtactgatgg ggggggcgat tggcggtctg tgtggattgc tggggtttgt
    18781 ggcaccagca acgtcgggcg gcggttttaa cctgattcct atcgctaccg cggggaattt
    18841 cagcatggga atgctggtgt ttatcttcgt cgctcgggtc attaccacct tactctgctt
    18901 ctcttccggc gcgccgggcg gtatttttgc cccgatgctg gcgctgggta ctgtgctggg
    18961 aaccgctttc ggaatggttg ccgttgagct gtttccgcaa tatcacctgg aggcggggac
    19021 gtttgctatt gccggaatgg gggcattact ggcggcatct attcgcgcgc cgttaacggg
    19081 gatcattctg gttctggaga tgaccgataa ctaccagctc attttgccaa tgattattac
    19141 cggtcttggc gcaacactat tagcgcaatt taccggcggg aaacctctat actcggcgat
    19201 tcttgcgcgc acgctggcaa aacaggaagc tgagcaactg gcgcgaagca aggccgcatc
    19261 agccagcgag aatacttgaa cgaaatacca gggtattaga taatggcgat tattattggg
    19321 ttagaatttg cccgattgcc gatgtcgttt ggagcaaaat atgagtgatg acgtagcact
    19381 gccgctggag tttaccgacg cagcagccaa caaagttaaa agcctgatcg ctgacgaaga
    19441 taacccgaat ctgaaattac gcgtgtatat caccggtggc ggttgcagcg gcttccagta
    19501 tggtttcacc tttgatgatc aggtgaacga aggcgatatg accatcgaaa aacagggcgt
    19561 tggcctggtg gttgatccga tgagcctgca atatctggtc ggcggttccg ttgactacac
    19621 cgaaggactg gaaggttctc gtttcatcgt gaccaacccg aacgcgaaaa gcacctgcgg
    19681 ttgcggttct tcctttagca tctaatctgt tgttcatgtg ccggataaac tatccggcac
    19741 atcttctctc ttcaacgccc attctcatcc agcgcaaacg tcggtagctt aagatgccaa
    19801 cgaatagccg ccagccgaat caatagcgtc acgaccatgc ccatcatact ggctgtttcc
    19861 agtggtacgg aaaatgtgta ataagccgta gcgtggacaa taccgccgat aatacaggca
    19921 gttgcgtaga tttctgtacg taaaatcatg gagatttcgc gggccagtac atcacgaatg
    19981 atcccgccgc caacgccagt aatgacgccc atacaaaccg cgattaacgg accggcttcc
    20041 gcattaaagg ctttattcac gccaatgccg acaaacaccg ccagaccaac ggcgtccagc
    20101 accggcaaca tccattttgg taagcgtctt ggctggcgca ccagcacgat ggtcagcatg
    20161 ctggtgacca tggcaacgac cagatcggtg ggatctttca cccaaaatac cgggccgtga
    20221 tccagcgcca tgtcgcgaat tgttccgtcg cctactgcgg taaccacgcc cagtaccaga
    20281 acaccaaaag ggtccatacg caattttccg gctaacaaaa cgccggagat ggcaaatacc
    20341 gctgtgccga ctatatccag ccaatagacg agcattgttc aatccccact gagcacctga
    20401 aaaggtcagg cactaatcta cctgtgaaag cgcattacag agctgttgtg ctgcgaggat
    20461 aatacgtggg cttgcacgtt caaaccagtc actcgtgaga ggaataacgg gaattttgag
    20521 ctgttcaccc cagtattgtt tgattttagg aatttggtcc ggtccgcctg taatgacaat
    20581 cgcctgtggc gagcgtgcta acacctgttc gcggctaact tgcggccagg gaacccggct
    20641 gtctttaaag atgttttctc cgccacaaac ttcgagtacc tggttctgaa tcgactcttt
    20701 tccactggta aatggcggat taatgccgaa ttgcagaaaa acacgttttt taggtttatc
    20761 agcatattgc gctttcaatt gcacgtactg atccagcagg gattgcgcgg cttgttcggc
    20821 cttgtctggt tgcggactcc agggggccag ttgacgtaac gcattggcaa tttgttcaat
    20881 gcttgtcgca tcgacccaca tcacttttat tcccagcgaa gccagctggt caacctgccg
    20941 ctcggcatta cctccacgcc aggcaatcac cagatcgggt ttcagcgcga caatgcgttc
    21001 cagattcatc ccctgccagg tggaaacctg ctcaatcttt tgcgcttgtg gaggatagtc
    21061 ggaatagctg ctgaccccaa ccggcgtgat cccggcggca aaggcaagtt cagtgttggc
    21121 gggagaaagc gtgatgacgc gcggcgcggc gttgagccac agtggcgcga gaaaagacag
    21181 ggcgaccagc gccctgaaca gtgatttagc catgtgccag tttctgcacc agtgactcaa
    21241 ccatcaggct ggactgttta gcggcaacag ccaggaactc atcgaagcta agatgagact
    21301 gttgatcggc cacgtcggag atggcgcgta cgacaacaaa cgggacgttg aaattgtggc
    21361 agacatgggc gattgccgtc gcttccatct ctacagcaat ggcctgtggg aagttgtggc
    21421 ggattttcgc cagaccaaca gaaccgttga tgaaagcgtc gccgctaaca atcaggccac
    21481 gtacagcgtt aagattcagt tcggcaatgc aggcctcagc ggcagcgatc agtttatcgt
    21541 cagctttaaa gcctgtcgga cagcctggta actgaccgta ttcataacca aatgccgtga
    21601 catccgcgtc gtgataacgc gcttcgtccg agacaacgat atcgcccact ttcagcgttg
    21661 gtgccaggcc accggcagaa ccggtgttaa taatcacatc tggcttgcag tgttccaaca
    21721 gcagagtggc acccagcgcc gcagcgactt taccgatgcc cgatttcaga agcgcaacct
    21781 cggttccatt cagttggccg gtatagattt cgcaaccgcc gagactgata gtttgacggt
    21841 tttcgatttt gtcacgcagc agcgtaactt cttcttccat tgcaccaatg atgccgattt
    21901 tcatagattt actcgcgata agcccgattt gaaggcatag tttaccatgc gcttacgggg
    21961 aagcgtattt ctcacgcggg agaggacatg gcacagattg atttccgaaa aaaaataaac
    22021 tggcatcgtc gttaccgttc accgcagggc gttaaaaccg aacatgagat cctgcggatc
    22081 ttcgagagcg atcgcgggcg tatcatcaac tctccggcaa ttcgtcgtct gcaacaaaag
    22141 acccaggttt ttccactgga gcgcaatgcc gccgtgcgca cgcgtcttac ccactcgatg
    22201 gaagtccagc aggtgggacg ctacatcgcc aaagaaattt taagccgtct gaaagagctt
    22261 aaattgctgg aagcatacgg cctggatgaa ctgaccggcc cctttgaaag cattgttgag
    22321 atgtcatgcc tgatgcacga tatcggcaat ccgccgtttg gtcattttgg cgaagcagcg
    22381 ataaatgact ggtttcgcca acgtttgcac ccggaagatg ccgaaagcca gcctctgact
    22441 gacgatcgct gcagcgtggc ggcactacgt ttacgggacg gggaagaacc gcttaacgag
    22501 ctgcggcgta agattcgtca ggacttatgt cattttgagg ggaatgcaca aggcattcgt
    22561 ctggtgcata cattgatgcg gatgaatctc acttgggcac aggttggcgg tattttaaaa
    22621 tatacccgtc cggcgtggtg gcgtggcgaa acgcctgaga cacatcacta tttaatgaaa
    22681 aagccgggtt attatctttc tgaagaagcc tatattgccc ggttgcgtaa agaacttaat
    22741 ttggcgcttt acagtcgttt tccattaacg tggattatgg aagctgccga cgacatctcc
    22801 tattgtgtgg cagaccttga agatgcggta gagaaaagaa tatttaccgt tgagcagctt
    22861 tatcatcatt tgcacgaagc gtggggccag catgagaaag gttcgctctt ttcgctggtg
    22921 gttgaaaatg cctgggaaaa atcacgctca aatagtttaa gccgcagtac ggaagatcag
    22981 ttttttatgt atttacgggt aaacacccta aataaactgg taccctacgc ggcacaacga
    23041 tttattgata atctgcctgc gattttcgcc ggaacgttta atcatgcatt attggaagat
    23101 gccagcgaat gcagcgatct tcttaagcta tataaaaatg tcgctgtaaa acatgtattt
    23161 agccatccgg atgtcgagca gcttgaattg cagggctatc gggtcattag cggattatta
    23221 gagatttatc gtcctttatt aagcctgtcg ttatcagact ttactgaact ggtagaaaaa
    23281 gaacgggtga aacgtttccc tattgagtcg cgcttattcc acaaactctc gacgcgccat
    23341 cggctggcct atgtcgaggc tgtcagtaaa ttaccgtcag attctcctga gtttccgcta
    23401 tgggaatatt attaccgttg ccgcctgctg caggattata tcagcggtat gaccgacctc
    23461 tatgcgtggg atgaataccg acgtctgatg gccgtagaac aataaccagg cttttgtaaa
    23521 gacgaacaat aaatttttac cttttgcaga aactttagtt cggaacttcg ggctataaaa
    23581 cgaatctgaa gaacacagca attttgcgtt atctgttaat cgagactgaa atacatgaaa
    23641 aaaaccacat tagcactgag tgcactggct ctgagtttag gtttggcgtt atctccgctc
    23701 tctgcaacgg cggctgagac ttcttcagca acgacagccc agcagatgcc aagccttgca
    23761 ccgatgctcg aaaaggtgat gccttcagtg gtcagcatta acgtagaagg tagcacaacc
    23821 gttaatacgc cgcgtatgcc gcgtaatttc cagcagttct tcggtgatga ttctccgttc
    23881 tgccaggaag gttctccgtt ccagagctct ccgttctgcc aggggggcca gggcggtaat
    23941 ggcggcggcc agcaacagaa attcatggcg ctgggttccg gcgtcatcat tgatgccgat
    24001 aaaggctatg tcgtcaccaa caaccacgtt gttgataacg cgacggtgat taaagtccag
    24061 ctgagcgatg gtcgtaagtt cgacgcgaag atggttggca aagatccgcg ctctgatatc
    24121 gcgctgatcc aaatccagaa cccgaaaaaa ctgaccgcaa ttaagatggc ggattctgat
    24181 gcactgcgcg tgggtgatta caccgtagcg attggtaacc cgtttggtct gggcgagacg
    24241 gtaacttccg ggattgtctc tgcgctgggg cgtagcggcc tgaatgctga aaactacgaa
    24301 aacttcatcc agaccgatgc agcgatcaac cgtggtaact ccggtggtgc gctggttaac
    24361 ctgaacggcg aactgatcgg tatcaacacc gcgatcctcg caccggacgg cggcaacatc
    24421 ggtatcggtt ttgctatccc gagtaacatg gtgaaaaacc tgacctcgca gatggtggaa
    24481 tacgggcagg tgaaacgcgg tgagctgggt attatgggga ctgagctgaa ttccgaactg
    24541 gcgaaagcga tgaaagttga cgcccagcgc ggtgctttcg taagccaggt tctgcctaat
    24601 tcctcggctg caaaagcggg cattaaagcg ggtgatgtga tcacctcact gaacggtaag
    24661 ccgatcagca gctttgccgc actgcgtgct caggtgggta ctatgccggt gggcagcaaa
    24721 ctgaccctgg gcttactgcg cgacggtaag caggttaacg tgaacctgga actgcagcag
    24781 agcagccaga atcaggttga ttccagctcc atcttcaacg gcattgaagg cgctgagatg
    24841 agcaacaaag gtaaagatca gggcgtggta gtgaacaacg tgaagacggg cactccggct
    24901 gcgcagatcg gcctgaagaa aggtgatgtg attattggtg cgaaccagca ggcagtgaaa
    24961 aacatcgctg aactgcgtaa agttctcgac agcaaaccgt ctgtgctggc actgaacatt
    25021 cagcgcggcg acagcaccat ctacctgtta atgcagtaat ctccctcaac cccttcctga
    25081 atacgggaag gggttctcct tacaatctgt gaacttcgcc acaactccat acatcttcat
    25141 catcctttgg gcatttgcac aatgccgtac gttacgtact tccttatgct aagccgtgca
    25201 taacggagga cttatggctg gctggcatct tgataccaaa atggcgcagg atatcgtggc
    25261 acgtaccatg cgcatcatcg ataccaatat caacgtaatg gatgcccgcg ggcgaattat
    25321 cggcagcggc gatcgtgagc gtattggtga attgcacgaa ggtgcattgc tggtactttc
    25381 acagggacga gtcgtcgata tcgatgacgc ggtagcacgt catctgcacg gtgtgcggca
    25441 ggggattaat ctaccgttac ggctggaagg tgaaattgtc ggcgtaattg gcctgacagg
    25501 tgaaccagag aatctgcgta aatatggcga actggtctgc atgacggctg aaatgatgct
    25561 ggaacagtcg cggttgatgc acttgttggc gcaggatagc cgtttgcggg aagaactggt
    25621 gatgaacctg attcaggcag aggagaatac tcccgcactt ctgaatgggc gcaacggctg
    25681 gggatcgatc tcaatcaacc gcgagtggtg gctattgttg aggtcgacag cggtcagctt
    25741 ggcgtggaca gcgcaatggc ggagttacaa caactgcaaa acgcgctgac tacgcccgag
    25801 cgtaataatc tggtggcgat tgtctcgcta accgaaatgg tggtgttgaa accggcgttg
    25861 aactcttttg ggcgctggga tgcagaagat catcgtaagc gagttgaaca actgattacc
    25921 cgcatgaaag agtacggcca gctgcgtttt cgcgtttcac tgggcaacta ttttaccggt
    25981 cctagcagta ttgcccgatc ctatcgtacg gcgaaaacga cgatggtggt gggtaaacag
    26041 cggatgccag aaagtcgctg ctatttttat caggatctga tgttacctgt gttactcgac
    26101 agtttgcgtg gcgactggca ggccaacgaa ctggcgcgac cgctggcgcg gctgaaagcg
    26161 atggacaata acggcttgct gcgacgaacg ctggcggcgt ggtttcgcca caatgtgcaa
    26221 ccgctggcaa cgtcaaaggc gttgtttatt catcgtaata ccctggagta tcggcttaat
    26281 cgtatatcgg aactgaccgg gcttgatttg ggcaattttg atgacaggtt gctgctgtat
    26341 gtggcgttac aactggatga agagcggtag attatgcgtt aaggttgagg cggtgctggc
    26401 tcattcccct cagcccgaag gggagagggg aatgcccgag ttaaccgtca attcgggatt
    26461 atttacgcgt tagtttttcc agatcggctt caatctcgct gatcttattg gttacgactg
    26521 actccaggtg acgtaagtcg tcgaggatct tacgcttaag atcaacttcg ctgcggtcac
    26581 gctgacagat ttgatcaagc tcatcaatga tataccgtag attcgggctg atttcctgga
    26641 cttctttata accctgaccc acaccatcag cgacgaccgt tttacgctga cgcggatatt
    26701 taaacttaac gctcttggcg aaaaactcgc ctttgtcttt ctggaaatag attttcagaa
    26761 tatcgttgtt ggcttcctgc cggaggctgt aacgatcaat ttcttcagga ttggtaatac
    26821 ccagactttt cagattgtcg tacatagcgt tacctcaaaa tgagtcagta aattgtgctt
    26881 attttagcat ttggcttggc ccgcccggct cgactgtgat cgcagaaagc ctttttcttc
    26941 cagacttcta tcatggcgta aaaatcaaaa aattacctgc tttattgtgg cgatattcca
    27001 tgggatggat tagcacattt atcttcgttt tgcgaattac atgacagtcc gaaacaagat
    27061 gaaaaagtat ttaaaaaagg gggaggaaga atgagtagga cgaattattc tgagccagaa
    27121 agcatacggg cagcatcatg ctgcccgtat atgtactctt aatcgatggt acgcagcagt
    27181 tcgttaatgc cgactttgcc gcgagttttc gcgtcaactt tcttaacgat aatcgcacag
    27241 tagaggctgt atttgccatc ttttgacggc agattacctg aaacaaccac agaccccgcc
    27301 ggaacgcgac cgtagtggat ttcgccggtt tcacggtcgt aaatacgggt gctctgacca
    27361 atgtatacgc ccatagagat gacggaacct tcttcgacaa tcaccccttc aaccacttca
    27421 gagcgtgcgc caatgaagca gttatcttca ataatggtcg ggttagcctg cagcggctcc
    27481 agcacgccgc cgatgcccac gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa
    27541 gaaccgacgg tcgcccaggt atcaaccatg gtgccttcat caacatatgc gccgatgtta
    27601 acgtaagacg gcatcagcac ggtgttacgg gcaataaacg caccctgacg taccgccgct
    27661 ggtggcacaa cgcggaagcc ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc
    27721 atcggcactt tgtcgaagta gcggctttct gccccttcga tcacctgatt atcattaata
    27781 cggaaagaga gcagcaccgc ttttttcaac cactgatgcg tcacccactg accgtcaatt
    27841 ttttccgcta cacgcagtgc gccggaatcc agcagggcga tcacctgatt taccgcttcg
    27901 cgggtaacgg tgtctgcatt ggctggcgtg atatcggcac ggcgttcaaa agcggtttca
    27961 ataatgttct gtaactgctg cattgttaaa ctcttttcat atcagtaaac acatcaccct
    28021 ttatcgtttg gattgagggc ctctgtcaac cgctgatgca cttcctgctg caactcgtta
    28081 ttaagcgcac gccggtcagc ggtggcaatt atgaataaat cttctactcg ctcgccaatg
    28141 gttgtaattc gggcaccatg aagcgaaatt cccagatcgg caaaaatttt cccgactcgc
    28201 gccagcagtc caggttggtc gagggcgatc agttcgagga acgatttgcg gtcggtatgg
    28261 gtcggcaaaa acgttacttc ggtttcaaca gtaaaatggc gtaatttggc gggttggcga
    28321 cggggctgcg gtggctgcca gctactttgc gtcagcacct gctccagacc aaaccgaata
    28381 acctcatggc gatctgcgga cagcgggctg ccatcgggtt ccagcacgat aaaggtatcc
    28441 atcgccatac cgtcgcgagt ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg
    28501 tctaattcgg cacagacggc ggcaaacaga taggggcggt ccgggctcca gataaaaatc
    28561 tcggtgcctc cacgcgtagc ctgcgggcta agcaatacca gcggtttgct taaatcatgc
    28621 tgtaataaat ggcgggcatg ccaggccagt tgatttgggc tatggcggac aaaatagtta
    28681 gcacgacagc gtgaccaaat ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt
    28741 agtgccagtg cctggagttg gtgatggcga acccgttcgc gcatatccgg cgtgttttgc
    28801 attccgcgtc gtagctgctt ttcggtggca aaatagagtt cgcgcaacag actttgcttc
    28861 cagctattcc acagcgtttc gttggtggcg caaatgtcag ccactgtcag gcataccaga
    28921 tagcgcagac gattttccgt ttgaacttct tcggcaaact gcttgatgac ttcagggtcc
    28981 tgaatatcgc ggcgttgtgc tgtcaccgac atcagcaagt gctggcgaac cagccaggca
    29041 accagctgtg tttcgcgtga gttcagcccg tggagttctg caaaatgcac tacatcctga
    29101 gcaccgagaa tggagtggtc gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg
    29161 atgaaaatca gctcagttga cggcaggcgc ggccagacgt ccacacacaa cggatggcgc
    29221 tggcgcgttt cttcactggc aaaactctcc agtttcagca tcacgcggat agtatgttca
    29281 tccaccgtgt aggcgtggaa cagatcaaac tgcatctgcc cgacgatatg cgaccattgc
    29341 ggcatatacg cgccgagcac gctatggcga tgcattggca atagcccgcg ccgcaccgct
    29401 ccggggtgac gcagaatgct caaaaacagt tttcgtgctt gcggaatatt acacagcggt
    29461 tgttgcagat ggcgacgggc atggcgtaac tggcgcagcg tggtggagta aataccggtg
    29521 atcgcactgt tgcgcaccat ggtgtagaac atacgcaaga tggcttccgg ctggcgcata
    29581 aatagtgttt catcacgcag gtcgattagc gtaccgcgta gctgaaactc atcgtcgagt
    29641 ggacgtggtt tttcgtcggc gggaagggcg aggatggctt catcgaacag ttgcagcagc
    29701 atctggttga gttcactgac gcggcgtgta acgcggaagt aatccttcat catccgctcg
    29761 accggttcgt tgccttcacc actgtaattc agacgctgag cgacgctaag ttggcgatcg
    29821 aataacagac gattatcgta acggctgacg accagatgca gggcaaagcg aatatgccac
    29881 agtatatgca gacattcgtt taattccgcc cgctccgctg aggttaagaa gccaaacccg
    29941 accatttcat ccagcgatgt tgcgccaaaa tgacggcggg ccacccattg cagagtgtga
    30001 atatcgcgca agccgccagg gctacttttg atgtctggtt caaggttgta gctggtgcca
    30061 tggtaacgct gatggcgctg gttctgttct tcaactttcg ctgcgtagaa cttgtcggaa
    30121 ggccagaatc cttcgctaaa aatatgtttt tgcagttcga ggaagagcgc aacatcgcca
    30181 attaataagc gggattcgat taaattggtg gcgacggtta aatccgataa cccttccaac
    30241 atgcactctt caagcgtgcg cacactatga ccgacttcca gctttacatc ccagagcagc
    30301 gttaacagct cgcccacttt ttgcgcctga tcgtccggga gctttttacg gcttaaaatc
    30361 agtaaatcga tgtcagaaag tggatgcagc tcgccacgac cgtagccacc gacggcaacc
    30421 aatgccaggt cggcaatctg gctgaatccc gcttcaatcc ataatcgttg caggagctgg
    30481 tctatgaact cggtacgtgc ctcaatcaac tgttctgcag agatcccatt gtcaaaggca
    30541 tcacccagcc aacgctggaa agtatcgata tgggctttta tcccacagac ggttaattca
    30601 tcacggggcc aggcgcatgg attttgcggt tgaccgggca gggtggagag agcggtgttt
    30661 gcgtactgtt ctggaagggt attcattgtg cgccacccat aagattaaat tatcgcatta
    30721 aaaaagccgg cattcgccgg cttcatctta ttcgtcgtgc gagattatcg ccgggatggt
    30781 gtcatccttg cgtagcgtca aaatttcgca gccgttatca gtcaccacaa tagtatgctc
    30841 atattgtgca gacaagctgc gatctttggt ttttaccgtc cagccatctt tcatggtgcg
    30901 gatctctttt ttacccgcgt tgaccattgg ctcaatggtg aacgtcatcc ccggtttcag
    30961 cacgacgttg gtttcacggg agtcatagtg cagcacctgc ggttcttcat ggaagccgcg
    31021 accaataccg tgtccgcaat attcacgaac gacggagaag ccttctgctt cgacaaattt
    31081 ctgaatcgcc gcaccgattt cgcgcagatt aatgcctggt tttaccatgc gtagcgccag
    31141 gtacaggctt tcttgcgtga tgcggcacag gcgttcgccc atgatggtcg gcttaccgac
    31201 gataaacatt ttcgaggtat cgccgtggaa accatctttg attacggtga catcaatgtt
    31261 aacgatatcg ccatctttca gcagcttagc atcgtccggg ataccgtggc acaccacttc
    31321 attaatagag atgcaaacgg atttcggata gccgtgatag ccgaggcagg cagaaaccgc
    31381 gtgttgttca ttaacaatgt aatcattaca gatgcgatcc agctcgccgg tgctgacgcc
    31441 cggtttaaca tacggttcga tcatctccag cacttcggca gccagtcggc cagcgacgcg
    31501 cattttttcg atatcttctg gggtcttgat tgagatagcc attaattctg tccatcagcg
    31561 tcggtgatac cgacaatata tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg
    31621 agaatcattc tgaatttcgc caaacgtgcc actgaaggtt ttctataata gaaaattcga
    31681 cgtctgatgc tgtacacagc gccaacaatt attggtgtcc acgacgtatt tgtggtataa
    31741 agcgcgccgg acttccgatc catttcgtat acacagactg gacggaagcg acaatctcac
    31801 tttgtgtaac aacacacacg tatcggcaca tattccgggg tgccctttgg ggtcggtaat
    31861 atgggatacg tggaggcata accccaactt ttatatagag gttttaatca tggcaactgt
    31921 ttccatgcgc gacatgctca aggctggtgt tcacttcggt caccagaccc gttactggaa
    31981 cccgaaaatg aagccgttca tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga
    32041 gaaaactgta ccgatgttca acgaagctct ggctgaactg aacaagattg tttctcgcaa
    32101 aggtaaaatc cttttcgttg gtactaaacg cgctgcaagc gaagcggtga aagacgctgc
    32161 tctgagctgc gaccagttct tcgtgaacca tcgctggctg ggcggtatgc tgactaactg
    32221 gaaaaccgtt cgtcagtcca tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg
    32281 tactttcgac aagctgacca agaaagaagc gctgatgcgc actcgtgagc tggagaaact
    32341 ggaaaacagc ctgggcggta tcaaagacat gggcggtctg ccggacgctc tgtttgtaat
    32401 cgatgctgac cacgaacaca ttgctatcaa agaagcaaac aacttgggta ttccggtatt
    32461 tgctatcgtt gataccaact ctgatccgga cggtgttgac ttcgttatcc cgggtaacga
    32521 cgacgcaatc cgtgctgtga ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg
    32581 ccgttctcag gatctggctt cccaggcgga agaaagcttc gtagaagctg agtaataagg
    32641 cttgataact cccccaaaat agttcgagtt gcagaaaggc ggcaagctcg agaattcccg
    32701 ggagcttaca tcagtaagtg accgggatga gcgagcgaag acaacgcatc tgcggcgcga
    32761 aatatgaagg gggagagccc ttatagacca ggtagtacac gtttggttag ggggcctgca
    32821 tatggccccc tttttcactt ttatatctgt gcggtttaat gccgggcaga tcacatctcc
    32881 gaggatttta gaatggctga aattaccgca tccctggtaa aagagctgcg tgagcgtact
    32941 ggcgcaggca tgatggattg caaaaaagca ctgactgaag ctaacggcga catcgagctg
    33001 gcaatcgaaa acatgcgtaa gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt
    33061 gctgctgacg gcgtgatcaa aaccaaaatc gacggcaact acggcatcat tctggaagtt
    33121 aactgccaga ctgacttcgt tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt
    33181 ctggacgcag ctgttgctgg caaaatcact gacgttgaag ttctgaaagc acagttcgaa
    33241 gaagaacgtg ttgcgctggt agcgaaaatt ggtgaaaaca tcaacattcg ccgcgttgct
    33301 gcgctggaag gcgacgttct gggttcttat cagcacggtg cgcgtatcgg cgttctggtt
    33361 gctgctaaag gcgctgacga agagctggtt aaacacatcg ctatgcacgt tgctgcaagc
    33421 aagccagaat tcatcaaacc agaagacgta tccgctgaag tggtagaaaa agaataccag
    33481 gtacagctgg atatcgcgat gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt
    33541 gaaggccgca tgaagaaatt caccggcgaa gtttctctga ccggtcagcc gttcgttatg
    33601 gaaccaagca aaactgttgg tcagctgctg aaagagcata acgctgaagt gactggcttc
    33661 atccgtttcg aagtgggtga aggcatcgag aaagttgaga ctgactttgc agcagaagtt
    33721 gctgcgatgt ccaagcagtc ttaattatca aaaaggagcc gcctgagggc ggcttctttt
    33781 tgtgcccatc ttgtaaattc agctaaccct tgtggggctg cgctgaaaag cgacgtacaa
    33841 tgtcgctagt attaattcat ttcaatcgtt gacagtctca ggaaagaaac atggctacca
    33901 atgcaaaacc cgtctataaa cgcattctgc ttaagttgag tggcgaagct ctgcagggca
    33961 ctgaaggctt cggtattgat gcaagcatac tggatcgtat ggctcaggaa atcaaagaac
    34021 tggttgaact gggtattcag gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg
    34081 ctggtctggc gaaagcgggt atgaaccgcg ttgtgggcga ccacatgggg atgctggcga
    34141 ccgtaatgaa cggcttggca atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc
    34201 tgatgtccgc tattccattg aatggcgtgt gcgacagcta cagctgggca gaagctatca
    34261 gcctgttgcg caacaaccgt gtggtgatcc tctccgccgg tacaggtaac ccgttcttta
    34321 ccaccgactc agcagcttgc ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag
    34381 caaccaaagt tgacggcgtg tttaccgctg atccggcgaa agatccaacc gcaaccatgt
    34441 acgagcaatt gacttacagc gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg
    34501 ccttcacgct ggctcgtgac cataaattac cgattcgtgt tttcaatatg aacaaaccgg
    34561 gtgcgctgcg ccgtgtggta atgggtgaaa aagaagggac tttaatcacg gaataattcc
    34621 cgtgatggat aaataagggt aagattccgc gtaagtatcg cgggggcgta agtctggtta
    34681 taaggcgtta ttgttgcagg cagtttggtc acggccagcg cgcagcaacc ggagcgtaca
    34741 aaagtacgtg aggatggcga gcactgctcg gggccaaaat ggcaaataaa atagcctaat
    34801 aatccagacg attacccgta atatgtttaa tcagggctat acttagcaca cttccactgt
    34861 gtgtgactgt ctggtctgac tgagacaagt tttcaaggat tcgtaacgtg attagcgata
    34921 tcagaaaaga tgctgaagta cgcatggaca aatgcgtaga agcgttcaaa acccaaatca
    34981 gcaaaatacg cacgggtcgt gcttctccca gcctgctgga tggcattgtc gtggaatatt
    35041 acggcacgcc gacgccgctg cgtcagctgg caagcgtaac ggtagaagat tcccgtactc
    35101 tgaaaatcaa cgtgtttgat cgttcaatgt ctccggccgt tgaaaaagcg attatggcct
    35161 ccgatctcgg cctgaacccg aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc
    35221 tgacggaaga acgtcgtaaa gatctgacca aaatcgttcg tggtgaagca gaacaagcgc
    35281 gtgttgcagt acgtaatgtg cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag
    35341 ataaagagat cagcgaagac gacgatcgcc gttctcagga cgatgtacag aaactgactg
    35401 atgctgcaat caagaaaatt gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt
    35461 tctgatttct tgaacgacaa aaacgccgct cagtagatcc ttgcggatcg gctggcggcg
    35521 ttttgctttt tattctgtct caactctgga tgtttcatga agcaactcac cattctgggc
    35581 tcgaccggct cgattggttg cagcacgctg gacgtggtgc gccataatcc cgaacacttc
    35641 cgcgtagttg cgctggtggc aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa
    35701 ttctctcccc gctatgccgt aatggacgat gaagcgagtg cgaaacttct taaaacgatg
    35761 ctacagcaac aggggagccg caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg
    35821 gcagcgcttg aggaggttga tcaggtgatg gcagccattg ttggtgctgc tgggctgtta
    35881 cctacgcttg ctgcgatccg cgcgggtaaa accattttgc tggccaataa agaatcactg
    35941 gttacctgcg gacgtctgtt tatggacgcc gtaaagcaga gcaaagcgca attgttaccg
    36001 gtcgatagcg aacataacgc tatttttcag agtttaccgc aacctatcca gcataatctg
    36061 ggatacgctg accttgagca aaatggcgtg gtgtccattt tacttaccgg gtctggtggc
    36121 cctttccgtg agacgccatt gcgcgatttg gcaacaatga cgcccgatca agcttgccgt
    36181 catccgaact ggtcgatggg acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa
    36241 ggtctggaat acattgaagc gcgttggctg tttaacgcca gcgccagcca gatggaagtg
    36301 ctgattcacc cgcagtcagt gattcactca atggtgcgct atcaggacgg cagtgttctg
    36361 gcgcagctgg gggaaccgga tatgcgtacg ccaattgccc acaccatggc atggccgaat
    36421 cgcgtgaact ctggcgtgaa gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc
    36481 gcaccggatt atgagcgtta tccatgcctg aaactggcga tggaggcgtt cgaacaaggc
    36541 caggcagcga cgacagcatt gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg
    36601 caacaaatcc gctttacgga tatcgctgcg ttgaatttat ccgtactgga aaaaatggat
    36661 atgcgcgaac cacaatgtgt ggacgatgtg ttatctgttg atgcgaacgc gagtgaagtc
    36721 gccagaaaag aggtgatgcg tctcgcaagc tgaggataat ccggctacag agagtcgcgc
    36781 tatttgttag cgtagggctt caatgatata gtctgcgcca tctgatcgta agtagttggc
    36841 tttataaggt caggtatgcc gtggttttac acggcttttt tttgtatagg cttcagtatt
    36901 cctgagtacc gtaaaccctg tcagggaata aaaaacgcgt gatgttgtct gctactcaac
    36961 cacttagcga aaaattgcca gcgcatggct gccgtcatgt tgcgatcatt atggacggca
    37021 atggtcgctg ggcaaaaaag caagggaaga ttcgtgcctt tgggcataaa gccggggcaa
    37081 aatccgtccg ccgggctgtc tcttttgcgg ccaacaacgg tattgaagcg ttaacgctgt
    37141 atgcctttag tagtgaaaac tggaaccgac cagcgcagga agtcagtgcg ttaatggaac
    37201 tgtttgtgtg ggcgctcgat agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc
    37261 gtattattgg cgataccagt cgttttaact cgcgtttgca agaacgtatt cgtaaatctg
    37321 aagcgctaac agccggaaat accggtctga cgctgaatat tgcggcgaac tacggtggac
    37381 gttgggatat agtccaggga gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc
    37441 aaccagatca gatagatgaa gagatgttaa accagcatgt ctgtatgcat gaactggccc
    37501 ctgtagattt agtaattagg actgggggag agcatcgcat tagtaacttt ttgctttggc
    37561 aaattgccta tgccgaactt tactttacag atgttctctg gcccgatttc gatgaacaag
    37621 actttgaagg ggcgttaaat gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc
    37681 ccggtgatga aacagcctga tgggggtcgc ttttgctgaa gtatcgcctg atatctgctt
    37741 ttgtgttaat acccgtcgtc atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca
    37801 ttgtaacgct ggtggtctgc atgctggcag cgtgggaatg gggacagctt agcggtttta
    37861 ccactcgttc gcagcgagta tggttggcgg tgttatgcgg gttattgttg gcgctgatgc
    37921 tttttctgtt gccggaatat caccgaaata ttcatcaacc gctggttgaa atctcacttt
    37981 gggcttcgtt gggttggtgg attgtcgcgc tattgctggt gctgttttac ccaggttccg
    38041 cagcaatctg gcgtaactct aaaacattgc gccttatttt tggcgtgcta accattgttc
    38101 ccttcttctg gggcatgctg gcgttacggg cctggcacta tgacgagaat cattacagtg
    38161 gcgcaatatg gctgctctat gtcatgatcc tggtatgggg cgctgactcc ggcgcatata
    38221 tgtttggcaa attgtttggt aaacataagc tggcaccgaa ggtttctccg ggtaaaacct
    38281 ggcaaggctt tataggtgga ctcgctactg cagcggtaat ctcatggggt tatggcatgt
    38341 gggcgaatct cgacgtcgct cccgtcacct tactcatttg ctctattgtc gcagcgttag
    38401 cctcagtgct cggcgatctg accgagagta tgtttaagcg tgaagcagga attaaggaca
    38461 gcggtcattt aattccagga cacggtggta ttttagatcg tattgatagc ctgacggctg
    38521 cggtaccggt ctttgcttgc ttgttgttac tggtattcag gacgctttaa cggaaggtaa
    38581 tatgctgagt tttctctggg atttggcttc gttcatcgtt gcactgggtg tacttatcac
    38641 cgtgcatgaa tttggtcatt tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt
    38701 ctcaataggg tttggtaagg cgctctggcg gcgaactgat aagctcggca ccgaatatgt
    38761 tatcgccctg atcccgttgg gcggttatgt caaaatgctg gatgagcgcg cagaaccggt
    38821 cgttccggaa ctccgccacc atgccttcaa taataaatct gtcggccaac gagtggcgat
    38881 tattgccgca ggtccggttg caaacttcat ttttgctatc tttgcctact ggctggtttt
    38941 tattattggt gtgcctggcg tacgtccggt ggttggcgaa atagcagcca attcgatagc
    39001 tgcggaagca caaattgcac caggtacgga actaaaagcc gtagatggta tcgaaacgcc
    39061 tgattgggat gccgtgcgtt tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat
    39121 tacagtagcg ccatttggca gcgaccaacg gcgggatgta aagctcgatt tacgtcactg
    39181 ggcgtttgag cctgataaag aagatccggt atcttcgctg gggattcgtc ctcgtgggcc
    39241 gcaaattgaa cctgtactgg aaaatgtgca gccaaactcg gcggcaagca aggcaggttt
    39301 gcaagcaggc gacaggatcg ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt
    39361 tgtgatgctt gtccgggata acccgggtaa atccttagcg ttagaaatcg aaaggcaggg
    39421 gagtcccttg tctttgacat taatcccgga gagtaaaccg ggtaatggta aagcgattgg
    39481 ttttgtcggt attgagccga aagtcattcc tttgccagat gagtataaag ttgtacgcca
    39541 gtatgggccg ttcaacgcca tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct
    39601 gacggtcagt atgctgggaa aattgatcac cggtgatgtg aaactgaaca acctcagtgg
    39661 gccgatttct atcgccaagg gggctgggat gacagcggaa ctcggggtag tttattacct
    39721 gccgtttctt gcgcttatta gcgtgaactt agggataatt aacctgtttc cgttgcccgt
    39781 acttgacggg gggcatctgc tgttccttgc gatcgaaaag atcaagggcg gaccggtatc
    39841 cgagcgggtt caagactttt gttatcgcat tggctcgatt ctgctggtgc tgttaatggg
    39901 gcttgcactt ttcaatgatt tctctcggtt atgagagtta gttaggaaga acgcataata
    39961 acgatggcga tgaaaaagtt gctcatagcg tcgctgctgt ttagcagcgc caccgtatac
    40021 ggtgctgaag ggttcgtagt gaaagatatt catttcgaag gccttcagcg tgtcgccgtt
    40081 ggtgcggccc tcctcagtat gccggtgcgt acaggcgaca cggttaatga tgaagatatc
    40141 agtaatacca ttcgcgctct gtttgctacc ggcaactttg aggatgttcg cgtccttcgt
    40201 gatggtgata cccttctggt tcaggtaaaa gaacgtccga ccattgccag cattactttc
    40261 tccggtaaca aatcggtgaa agatgacatg ctaaagcaaa acctcgaggc ttctggtgtg
    40321 cgtgtgggcg aatccctcga tcgcaccacc attgccgata tcgagaaagg tctggaagac
    40381 ttctactaca gcgtcggtaa atatagcgcc agcgttaaag ctgtcgtgac cccgctgccg
    40441 cgcaaccgtg ttgacctaaa actggtgttc cgggaaggtg tgtcagctga aatccagcaa
    40501 attaacattg ttggtaacca tgctttcacc accgacgaac tgatctctca tttccaactg
    40561 cgtgacgaag tgccgtggtg gaatgtggta ggcgatcgta aataccagaa acagaaactg
    40621 gcgggcgacc ttgaaaccct gcgcagctac tatctggacc gcggttatgc ccgtttcaac
    40681 atcgactcta cccaggtcag tctgacgcca gataaaaaag gtatttacgt cacggtgaac
    40741 atcaccgaag gcgatcagta caagctttct ggcgttgaag tgagcggcaa ccttgccggg
    40801 cactccgctg aaattgagca gctgactaag atcgagccgg gcgagctgta taacggcact
    40861 aaagtgacca aaatggaaga tgacattaaa aagcttctcg gtcgctatgg ttatgcctat
    40921 ccgcgcgtac agtcgatgcc tgaaattaac gatgccgaca aaaccgttaa attacgcgtg
    40981 aacgttgatg cgggtaaccg tttctacgtg cgtaagatcc gttttgaagg taacgatacc
    41041 tcgaaagatg ccgtcctgcg tcgcgaaatg cgtcagatgg aaggtgcatg gttggggagc
    41101 gatctggtcg atcagggtaa ggagcgtctg aatcgtctgg gcttctttga aactgtcgat
    41161 actgataccc aacgtgttcc gggtagcccg gaccaggttg atgtagtcta caaggtgaaa
    41221 gaacgtaaca ctggtagctt caacttcggg attggttacg gtactgaaag tggcgtgagc
    41281 ttccaggctg gcgtgcaaca ggataactgg ttaggtacag gttatgctgt tggtatcaac
    41341 gggaccaaaa acgattacca gacctatgct gaactgtcgg taaccaaccc gtacttcacc
    41401 gtagatggcg taagcctcgg tggtcgtctc ttctataatg acttccaggc agatgacgcc
    41461 gacctgtccg actataccaa caagagttat ggtacagacg tgacgttggg cttcccgatt
    41521 aacgaatata actcgctgcg tgcaggtctg ggttatgtac ataactccct gtccaacatg
    41581 cagcctcagg ttgcgatgtg gcgttatctg tactctatgg gtgaacatcc gagcacttct
    41641 gatcaggata acagcttcaa aacggacgac ttcacgttca actatggttg gacctataac
    41701 aagcttgacc gtggttactt cccgacagat ggttcacgtg tcaacctgac cggtaaagtg
    41761 accattcctg gatcggataa cgaatactac aaagtgacgt tagacacggc gacttatgtg
    41821 ccgatcgatg acgatcacaa atgggttgtt ctgggtcgta cccgctgggg ttatggtgat
    41881 ggtttaggcg gcaaagagat gccgttctac gagaacttct atgccggtgg ttccagcacc
    41941 gtgcgtggct tccagtccaa taccattggt ccgaaagcag tttacttccc gcatcaggcc
    42001 agtaattatg atccggacta tgattacgaa tgtgcgactc aggacggcgc gaaagacctg
    42061 tgtaaatcgg atgatgctgt aggcggtaac gccatggcgg ttgccagcct cgagttcatc
    42121 accccgacgc cgtttattag cgataagtat gctaactcgg ttcgtacttc attcttctgg
    42181 gatatgggta ccgtttggga tacaaactgg gattccagcc aatattctgg atatccggac
    42241 tatagtgatc caagcaatat ccgtatgtct gcgggtatcg cattacaatg gatgtcccca
    42301 ttggggccgt tggtgttctc ctacgcccag ccgttcaaaa agtacgatgg agacaaggca
    42361 gaacagttcc agtttaacat cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt
    42421 ggtagtgtag cgatgacttt aggcgatcaa tataagatcg ccgggccacg caaagaactg
    42481 caccctccgg tgcaaatggg atggtaagga gtttattgtg aaaaagtggt tattagctgc
    42541 aggtcttggt ttggcactgg caacttctgc tcaggcggct gacaaaattg caatcgtcaa
    42601 tatgggcagc ctgttccagc aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa
    42661 tgagttcaaa ggccgtgcca gcgaactgca gcgtatggaa accgatctgc aggctaaaat
    42721 gaaaaaactg cagtccatga aagcgggcag cgatcgcacc aaactggaaa aagacgtgat
    42781 ggctcagcgc cagacttttg ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg
    42841 ttccaacgaa gaacgcggca aactggttac tcgtatccag actgctgtga aatccgttgc
    42901 caacagccag gatatcgatc tggttgttga tgcaaacgct gttgcttaca acagcagcga
    42961 tgtaaaagac atcactgccg acgtactgaa acaggttaaa taagtaatgc cttcaattcg
    43021 actggctgat ttagcgcagc agttggatgc agaactacac ggtgatggcg atatcgtcat
    43081 caccggcgtt gcgtccatgc aatctgcaca aacaggtcac attacgttca tggttaaccc
    43141 aaaataccgt gagcatttag gcttgtgcca agcgtccgcg gttgtcatga cccaggacga
    43201 tcttcctttc gcgaaaagtg ccgcgctggt agtgaagaat ccctacctga cttacgcgcg
    43261 catggcgcaa attttagata ccacgccgca gcccgcgcag aacattgcac ccagtgcggt
    43321 gatcgacgcg acggcgaagc tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga
    43381 gtccggcgtt gaactgggcg ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa
    43441 cagcaaaatc ggtgcaggtt cgcgtctctg ggcgaacgta accatttacc atgagatcca
    43501 gatcggtcag aattgcctga tccagtccgg aacagtggta ggcgcagacg gctttggtta
    43561 tgccaacgat cgtggtaact gggtgaagat cccacagatt ggtcgcgtaa ttattggcga
    43621 tcgcgtggag atcggtgcct gcacaaccat cgatcgcggc gcgctggatg acactgttat
    43681 tggcaatggc gtgatcattg ataaccagtg ccagattgca cataacgtcg tgattggcga
    43741 caatacggcg gttgccggtg gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg
    43801 catgatcggc ggagccagcg taatcaacgg gcatatggaa atatgcgaca aagtgacggt
    43861 tacgggcatg ggtatggtga tgcgtcccat cactgaacca ggcgtctatt cctcaggcat
    43921 tccgctgcaa cccaacaaag tctggcgcaa aaccgctgca ctggtgatga acattgatga
    43981 catgagcaag cgtctgaaat cgcttgagcg caaggttaat caacaagact aacgttccat
    44041 cttttgttcg ccaaacttta cggcctgtct cattcttacg attgcggcag gccgtgttat
    44101 tattgtcgtt tcttatattt tgacaggaag agtatcttga ctactaacac tcatactctg
    44161 cagattgaag agattttaga acttctgccg caccgtttcc cgttcttact ggtggatcgc
    44221 gtgctggatt ttgaagaagg tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag
    44281 ccattcttcc agggccattt ccctggaaaa ccgattttcc cgggtgtgct gattctggaa
    44341 gcaatggcac aggcaacagg tattctggcg tttaaaagcg taggaaaact ggaaccgggt
    44401 gagctgtact acttcgctgg tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc
    44461 gatcaaatga tcatggaagt cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa
    44521 ggggttgctc tggtcgatgg taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc
    44581 cgggaggcct gatacgtgat tgataaatcc gcctttgtgc atccaaccgc cattgtggaa
    44641 gagggcgcgt caattggcgc gaacgcacac attggtcctt tttgtatcgt tggaccccat
    44701 gtcgaaattg gtgagggtac cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa
    44761 attggccgcg ataatgagat ttatcagttt gcctccatcg gcgaagttaa ccaggatttg
    44821 aaatatgctg gcgaaccgac ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc
    44881 gtcaccattc atcgtggcac agtccagggc ggtggattga cgaaggtggg cagcgacaac
    44941 ttactgatga tcaacgcgca cattgcgcac gattgtacgg taggtaaccg ctgtattctc
    45001 gccaacaacg caacgctggc gggtcacgta tcggttgacg acttcgcgat catcggcggc
    45061 atgaccgcag tccatcagtt ctgcatcatt ggtgcgcatg tgatggttgg cggctgctcc
    45121 ggtgtggcgc aggacgtccc tccttatgtc attgcgcagg gtaaccacgc aacgccgttc
    45181 ggtgtcaata tcgaagggct gaagcgccgc ggattcagcc gtgaggcgat taccgctatc
    45241 cgcaatgcgt ataagctaat ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa
    45301 attgctgaac tggcggaaac atatccggaa gtgaaagcct ttaccgattt ctttgcacgc
    45361 tcaacgcgcg gtctgattcg ttaatgactg accagcgtcc attaacgatt gccctggtcg
    45421 ccggagaaac ctccggcgat atcctggggg ccggtttaat ccgcgctctg aaagaacgtg
    45481 tgcccaacgc ccgctttgtt ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag
    45541 cctggtacga aatggaagaa ctggcggtga tgggcattgt tgaagtgctc ggtcgtctgc
    45601 gtcgcttact gcatattcgt gccgatctga caaagcgttt tggcgaactg aagccagatg
    45661 tttttgttgg tattgatgcg cccgacttca atattactct tgaaggtaac ctcaaaaagc
    45721 agggtatcaa aaccattcat tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg
    45781 ttttcaaaat aggcagagcc accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt
    45841 tttatgacaa atacaatgta ccgtgccgct ttatcggtca taccatggct gatgccatgc
    45901 cgttagatcc agataaaaat ggtgcccgtg atgtgctggg gatcccttac gatgcccact
    45961 gtctggcatt gttgccgggc agccgtggtg cagaagtcga aatgcttagt gccgatttct
    46021 tgaaaactgc ccagcttttg cgccagacat atccggatct cgaaatcgtg gtgccgctgg
    46081 tgaatgccaa acgccgcgag cagtttgaac gcatcaaagc tgaagtcgcg ccagacctgt
    46141 cggttcattt gctggatgga atgggccgtg aggcgatggt cgccagcgat gcggcactac
    46201 tggcgtcggg gacggcagcc ctggagtgta tgctggcgaa atgcccgatg gtggtgggat
    46261 atcgcatgaa gccttttacc ttctggttgg cgaagcggct ggtgaaaact gattatgtct
    46321 cgctgccaaa tctgctggcg ggcagagagt tagtcaaaga gttattgcag gaagagtgtg
    46381 agccgcaaaa actggctgcg gcgctgttac cgctgttggc gaacgggaaa accagccacg
    46441 cgatgcacga taccttccgt gaactgcatc agcagatccg ctgcaatgcc gatgagcagg
    46501 cggcacaagc cgttctggag ttagcacaat gatcgaattt gtttatccgc acacgcagct
    46561 ggttgcgggt gtggatgaag tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc
    46621 ggtgatcctt gacccggcgc gcccgattgc cgggctgaat gattccaaaa agctgagcga
    46681 aaaacgccgt ctggcgctct atgaagagat caaagagaaa gcgttgagct ggagtctggg
    46741 ccgcgcggaa ccccacgaaa tcgacgagct gaacattctt catgcgacca tgctggcgat
    46801 gcagcgtgcc gtcgctgggc tgcatattgc gccggaatat gtgttgattg atggtaaccg
    46861 ctgcccgaaa ttaccgatgc ctgcgatggc tgtggtgaaa ggggatagcc gcgtaccaga
    46921 aatcagtgcc gcgtctatcc tagcgaaagt gacgcgtgac gccgaaatgg cggcgctgga
    46981 tattgttttc ccgcaatatg gttttgccca acataaaggg tatccaaccg cttttcatct
    47041 ggaaaaactg gctgaatatg gcgcgactga acatcatcgt cgcagctttg ggcctgtcaa
    47101 acgcgcactg ggacttgcgt cctgattctt gtgtcgagat taagtaaacc ggaatctgaa
    47161 gatgtctgaa ccacgtttcg tacacctgcg ggtgcacagc gactactcga tgatcgatgg
    47221 cttggccaaa accgcaccgc tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc
    47281 gatcaccgat ttcaccaacc tttgcggtct ggtgaagttc tacggagcgg gacatggcgc
    47341 agggattaag cctatcgtcg gggcagattt taatgtccag tgcgacctgc tgggtgatga
    47401 gttaacccac ctgacggtac tggcggcgaa caataccggc tatcagaatc tggcgttgct
    47461 gatctcaaaa gcgtatcagc gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg
    47521 gcttatcgaa ttaaacgaag ggttgatcct tctttccggc gggcgcatgg gcgacgtcgg
    47581 acgcagtctt ttgcgtggta acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga
    47641 acacttcccg gatcgctatt ttctcgagct gatccgcacc ggcaggccgg atgaagaaag
    47701 ctatctgcac gcggcggtgg aactggcgga agcgcgcggt ttgcccgtcg tggcgactaa
    47761 cgatgtgcgc tttatcgaca gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca
    47821 cgacggcttt accctcgacg atcctaaacg cccgcgtaac tattcgccgc agcaatatat
    47881 gcgtagcgaa gaggagatgt gtgagctgtt tgccgacatc ccagaagccc ttgccaacac
    47941 cgttgagatc gccaaacgct gtaacgtaac tgtgcgtctt ggtgaatact tcctgccgca
    48001 gttcccgacc ggggacatga gcaccgaaga ttatctggtc aagcgtgcaa aagaaggcct
    48061 ggaagagcgt ctggcatttt tattccctga cgaggaagaa cgtcttaaac gccgcccgga
    48121 atatgacgaa cgtctggaga ctgaacttca ggttatcaac cagatgggtt tcccgggcta
    48181 cttcctcatc gttatggaat ttatccagtg gtcgaaagat aacggcgtac cggtaggacc
    48241 aggccgtggc tccggtgcgg gttcactggt ggcctacgcg ctgaaaatca ccgacctcga
    48301 tccgctggaa tttgacctgc tgttcgaacg tttccttaac ccggaacgtg tctccatgcc
    48361 tgacttcgac gttgacttct gtatggagaa acgcgatcag gttatcgaac acgtggcgga
    48421 catgtacggt cgtgatgcgg tatcgcagat tatcaccttc ggtacgatgg cggcgaaagc
    48481 ggtgatccgc gacgtaggcc gcgtgctggg gcatccgtac ggctttgtcg accgtatctc
    48541 gaaactgatc ccgcccgatc cggggatgac gctggcgaaa gcgtttgaag ccgagccgca
    48601 gctgccggaa atctacgaag cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa
    48661 actggaaggg gtcacccgta acgccggtaa gcacgccggt ggggtggtta tcgcgccgac
    48721 caaaattacc gattttgcgc cgctttattg cgatgaagag ggcaaacatc cggtcaccca
    48781 gtttgataaa agcgacgttg aatacgccgg actggtgaag ttcgacttcc ttggtttgcg
    48841 tacgctcacc atcatcaact gggcgctgga gatgatcaac aagcggcggg cgaagaatgg
    48901 cgagccaccg ctggatatcg ccgcgatccc gctggatgac aagaaaagct tcgacatgct
    48961 gcaacgctcg gaaaccacgg cggtattcca gcttgaatcg cgcggcatga aggacctgat
    49021 caagcgtctg caacctgact gcttcgaaga tatgatcgca ctggtggcac tgttccgccc
    49081 tggtccgttg cagtcaggga tggtggataa ctttatcgac cgtaaacatg gtcgtgaaga
    49141 gatctcctat ccggacgtgc agtggcagca tgaaagcctg aaatcggtac tggagccaac
    49201 ctacggcatt atcttgtatc aggaacaggt catgcagatt gcccaggtgc tttctggtta
    49261 taccctcggt ggcgcggata tgctgcgccg tgcgatgggt aagaaaaagc cggaagagat
    49321 ggctaagcag cgttctgtat ttgctgaagg tgcagaaaag aacggaatca acgccgaact
    49381 ggcgatgaaa atcttcgacc tggtggagaa attcgcgggt tacggattta acaaatcgca
    49441 ctctgcggcc tatgctttgg tgtcatatca aacgttatgg ctgaaagcgc actatccggc
    49501 ggagtttatg gcggcggtaa tgaccgccga tatggacaac accgagaagg tggtgggcct
    49561 ggtggatgag tgctggcgga tggggctgaa aatcctgcca ccagatataa actccggtct
    49621 ttaccatttc cacgtcaacg acgacggcga aatcgtgtat ggtattggcg ccatcaaagg
    49681 ggtaggtgaa ggtccgattg aggccatcat cgaagcccgt aataaaggcg gctacttccg
    49741 cgaactgttt gatctctgcg cccgtaccga caccaaaaag ttaaaccggc gggtgctgga
    49801 aaaactgatc atgtccgggg cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa
    49861 ctcgctgggt gatgcgttaa aagcggcaga tcaacacgcg aaagcggaag ctatcggtca
    49921 ggccgatatg ttcggcgtgc tggccgaaga gccggaacaa attgaacaat cctacgccag
    49981 ctgccaaccg tggccggagc aggtggtatt agatggggaa cgtgaaacgt taggcctgta
    50041 cctgaccgga caccctatca accagtattt aaaagagatt gagcgttatg tcggaggcgt
    50101 aaggctgaaa gacatgcacc cgacagaacg tggtaaagtc atcacagctg cggggctcgt
    50161 tgttgccgcg cgggttatgg tcaccaagcg cggcaatcgt atcggtatct gcacgctgga
    50221 tgaccgttcc gggcggctgg aagtgatgtt gtttactgac gccctggata aataccagca
    50281 attgctggaa aaagaccgca tacttatcgt cagcggacag gtcagctttg atgacttcag
    50341 cggtgggctt aaaatgaccg ctcgcgaagt gatggatatt gacgaagccc gggaaaaata
    50401 tgctcgcggg cttgctatct cgctgacgga caggcaaatt gatgaccagc ttttaaaccg
    50461 actccgtcag tctctggaac cccaccgctc tgggacaatt ccagtacatc tctactatca
    50521 gagggcggat gcacgcgcgc ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga
    50581 tcgtttatta aacgatctcc gtggcctcat tggttcggag caggtggaac tggagtttga
    50641 ctaatacagg aatactatga gtctgaattt ccttgatttt gaacagccga ttgcagagct
    50701 ggaagcgaaa atcgattctc tgactgcggt tagccgtcag gatgagaaac tggatattaa
    50761 catcgatgaa gaagtgcatc gtctgcgtga aaaaagcgta gaactgacgc gtaaaatctt
    50821 cgccgatctc ggtgcatggc agattgcgca actggcacgc catccgcagc gtccttatac
    50881 cctagattac gttcgcctgg catttgatga atttgacgaa ctggctggcg accgtgcgta
    50941 tgcagacgat aaagctatcg tcggtggtat cgcccgtctc gatggtcgtc cggtgatgat
    51001 cattggtcat caaaaaggtc gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc
    51061 agcgccagaa ggttaccgca aagcactgcg tctgatgcaa atggctgaac gctttaagat
    51121 gcctatcatc acctttatcg acaccccggg ggcttatcct ggcgtgggcg cagaagagcg
    51181 tggtcagtct gaagccattg cacgcaacct gcgtgaaatg tctcgccttg gcgtaccggt
    51241 agtttgtacg gttatcggtg aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga
    51301 taaagtgaat atgctgcaat acagcaccta ttccgttatc tcgccggaag gttgtgcgtc
    51361 cattctgtgg aagagcgccg acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc
    51421 tccgcgtctg aaagagctga aactgatcga ctccatcatc ccggaaccgc tgggcggcgc
    51481 tcaccgtaac ccggaagcga tggcggcatc gttgaaagcg caactgctgg cggatctggc
    51541 cgatctcgat gtgttaagca ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag
    51601 ctacggttac gcgtaattcg caaaagttct gaaaaagggt cacttcggtg gccctttttt
    51661 atcgccacgg tttgagcagg ctatgattaa ggaaggattt tccaggagga acacatgaac
    51721 atcattgcca ttatgggacc gcatggcgtc ttttataaag atgagcccat caaagaactg
    51781 gagtcggcgc tggtggcgca aggctttcag attatctggc cacaaaacag cgttgatttg
    51841 ctgaaattta tcgaacataa ccctcgaatt tgcggcgtga tttttgactg ggatgagtac
    51901 agtctcgatt tatgcagcga tatcaatcaa cttaatgaat atctcccgct ttatgccttc
    51961 atcaacaccc actcgacgat ggatgtcagc gtgcaggata tgcggatggc gctctggttt
    52021 tttgaatatg cgctggggca ggcggaagat atcgccattc gtatgcgtca gtacaccaac
    52081 gaatatcttg ataacattac gccgccgttc acgaaagcct tgtttaccta cgtcaaagag
    52141 cggaagtaca ccttttgtac gccggggcat atgggcggca ccgcatatca aaaaagcccg
    52201 gttggctgtc tgttttatga ttttttcggc gggaataccc ttaaggctga tgtctctatt
    52261 tcggtcaccg agcttggttc gttgcttgac cacaccgggc cacacctgga agcggaagag
    52321 tacatcgcgc ggacttttgg cgcggaacag agttatatcg ttaccaacgg aacatcgacg
    52381 tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca gtacgctgtt gatcgaccgc
    52441 aactgccata aatcactggc gcatctgttg atgatgaacg atgtagtgcc agtctggctg
    52501 aaaccgacgc gtaatgcgtt ggggattctg ggggggatcc cgcgccgtga atttactcgc
    52561 gacagcatcg aagagaaagt cgctgccact acgcaagcac aatggccggt tcatgcggtg
    52621 atcaccaact ccacctatga tggtttgctc tacaacaccg actggatcaa acagacgctg
    52681 gatgtcccgt cgatccactt cgattccgcc tgggtgccgt atactcattt tcatccaatc
    52741 taccagggta aaagtggtat gagcggcgag cgtgttgcgg gaaaagtttt cttcgaaacg
    52801 caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt cactgatcca cattaaaggc
    52861 gagtatgacg aagaggcgtt taacgaagcc tttatgatgc ataccaccac ctcgcccagt
    52921 tatcccattg ttgcttcggt tgagacggcg gcggcgatgc tgcgtggtaa tccgggcaaa
    52981 cggctgatta accgttcggt ggagcgggcg ctgcattttc gcaaagaggt ccagcggctg
    53041 cgggaagagt ctgacggttg gtttttcgat atctggcaac cgccgcaggt ggatgaagcc
    53101 gaatgctggc ccgttgcgcc tggcgaacaa tggcacggct ttaacgatgc ggatgccgat
    53161 catatgtttc tcgatccggt taaagtcact attttgacac cggggatgga cgaacagggc
    53221 aatatgagtg aggaggggat cccggcggcg ctggtagcaa aattcctcga cgaacgtggg
    53281 atcgtagtag agaaaaccgg cccttataac ctgctgtttc tctttagtat tggcatcgat
    53341 aaaaccaaag caatgggatt attgcgtggg ttgacggaat ttaagcgctc ttacgatctc
    53401 aacctgcgga tcaaaaatat gctgcccgat ctctatgctg aagatcccga tttctaccgc
    53461 aatatgcgta ttcaggatct ggcgcaaggg atccataagc tgattcgtaa acacgatctt
    53521 tccggtttga tgttgcgggc attcgatact ttgccggaaa tgatcatgac gccacatcag
    53581 gcatggcaga gacagattaa aggtgaagta gaaaccattg cgctggaaca actggtcggt
    53641 agagtatcgg caaatatgat cctgccttat ccaccgggcg taccgctgtt gatgccagga
    53701 gaaatgctga ccaaagagag ccgcacggta ctcgattttc tactgatgct ttgttccgta
    53761 gggcaacatt accccggttt tgaaacggat attcacggcg cgaaacagga cgaagacggc
    53821 gtttaccgcg tacgagtcct aaaaatggcg ggataacttg ccagagcggc ttccgggcga
    53881 gtaacgtgct gttaacaaat aaaggagacg ttatgctggg tttaaaacag gttcaccata
    53941 ttgcgattat tgcgacggat tatgcggtga gcaaagcttt ctactgcgat attcttggtt
    54001 tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc gtggaaaggg gatttggcgc
    54061 ttaatgggca atatgtgatt gagcttttct cttttccgtt cccgccggag cgccccagcc
    54121 gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt tgatgacatc gatgcggcag
    54181 tggcgcacct tgaaagccat aacgtgaagt gtgaagccat ccgtgtcgat ccatacacgc
    54241 aaaaacgctt caccttcttt aacgatccgg acgggctgcc gttggaactg tatgagcagt
    54301 aaggcttgtc atcgccgcat ttgcccggta acgtgccggg cattgctact gtaaaatcgc
    54361 accatcatga cactcacgct caatagacca cttctcacct cacgccagat tctggtggcc
    54421 tttagcggcg ggcttgactc caccgttctg ctgcatcagt tggtgcagtg gcggacggaa
    54481 aatccgggcg tcactctgcg cgctattcat gtgcatcacg gtttaagtgc caatgccgat
    54541 gcctgggtta cgcattgcga aaacgtctgc caacagtggc aggtgccgct ggtggtcgaa
    54601 cgcgtacaac ttgcgcaaga ggggctgggg attgaggccc aggcgcggca ggcgcgttat
    54661 caggcatttg cccgtaccct attgcccggc gaagtgctgg tcaccgcgca acatctcgac
    54721 gatcaatgtg aaacctttct gctggcgcta aaacgcggta gcggccctgc cgggctttcg
    54781 gctatggcgg aagtttcgga gtttgctgga acacggctta tccgcccgtt gcttgcccgc
    54841 acgcgggggg aactggagca gtgggcattg gcgcatggtt tacgctggat tgaagacgaa
    54901 agtaatcagg acgactcata cgatcgtaac tttctgcgcc tgcgcgtagt gccgttattg
    54961 cagcagcgtt ggccgcattt tgcggaagca acggcccgca gcgccacact ttgtgctgaa
    55021 caagagagtc tgttggatga actgctggcg gatgatttag cacactgcca aacgtcgcag
    55081 gggacgctgc agattgcgcc aatgctggcg atgagtgatg cccgtcgcgc ggcgattatt
    55141 cgccgctggc tggcagggca gaatgcaccg atgccttccc gcgacgcgct ggtgcgcatc
    55201 tggcaggaag tggcgctggc gcgggaagat gcttccccct gtttacgttt gggcgccttt
    55261 gaaatccgcc gctatcagtc gcaactgtgg tggattaaat ccgtcaccgg gcaaagcgaa
    55321 accattgtgc tgtggcagac gtggctacaa ccgctggaat taccggcggg gctgggaacc
    55381 gtacagctta ctgcgggagg cgatattcgc cctccgcgtg cagacgaagc ggtcagcgtg
    55441 cgtttcaaag cgccaggatt gctgcatatt gtcgggcgca acggcggacg taagctgaag
    55501 aaaatctggc aagagttagg cgtgccgccg tggctacgtg acaccacgcc actgctgttt
    55561 tatggagaaa cgctgattgc ggcggcaggg gtatttgtga cgcaagaagg tgtggctgaa
    55621 ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta gttaagtgaa agccggataa
    55681 gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat tcgcttacca ccaccgtacc
    55741 gatttcaggg tggctaaagc tggtaatttt atccagacgc agctcgcggg tttcgccagc
    55801 ggcctcgacg accagatact ccacattttt gcgggagact aaatcactgg cttttgcctg
    55861 caatttttcg ccatctttca gctcaagtgt cagcattaaa tgatgctggc aggcgagctc
    55921 aagattatcg taatcatcac aattaattgg ttgatacgta tcattcattg acataatcgc
    55981 tcaccagtaa gtttgccgca gcgtatgctg ctttttccct gacagcctca gaaagggcgt
    56041 cgtcggcagc catttcattc agcactttca aaacgcagcc cagcgcgtcc ggaacgtatc
    56101 ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat taactcacaa tatttttcca
    56161 catgccctcc tgtcagcact ctgacttaac cgtggatgca agtctaagcc tacgaagata
    56221 aactctgttt cgcaaggtga ctataccaca ctcatttctg caatatcagc gccgcaactg
    56281 cacgtattcc gttacaatgg cctcctgatt cgaaaggagt tttcttatgg cgcttaaagc
    56341 gacaatttat aaagcgacgg ttaatgtggc cgatctcgac cgcaaccagt ttctcgatgc
    56401 ttctctgacg ctggcgcgcc acccttcaga aacccaggag cgtatgatgc tgcgcttgct
    56461 ggcgtggctg aaatatgctg atgaacgtct gcaatttacc cgtggtttgt gtgccgatga
    56521 tgagccggaa gcgtggctgc gtaacgatca cctgggcatt gatttgtgga ttgagctggg
    56581 gctgccggat gagcgacgaa ttaagaaagc ctgcacccag gccgcagaag tggcgctgtt
    56641 tacctataat agtcgggcgg cgcaaatctg gtggcagcaa aatcagagca aatgtgtgca
    56701 gtttgccaat ctttccgtct ggtatctgga cgatgaacaa ctggcgaaag taagcgcctt
    56761 tgccgatcgt accatgacgc tgcaggcaac gattcaggat ggtgttatct ggttatcgga
    56821 tgataagaat aatctggaag ttaacttaac cgcctggcaa caaccttcat gattgtgatt
    56881 tcccgacatg ttgctattcc cgatggtgag cttgagatca ccgccattcg tgcgcagggc
    56941 gctggcgggc agcatgttaa taagacctca acggctattc atctgcgttt tgacattcgg
    57001 gcgcccagcc tgccagagta ttacaaagag cgtctgctcg ccgccagcca tcatttgatc
    57061 agcagtgatg gcgtgattgt cattaaggca caggaatacc gcagtcagga actgaaccgc
    57121 gaagccgctc tggcccggct ggtggcagtg attaaagatt taacaaaaga acaaaaagcc
    57181 cgacgaccca cgcggcccac ccgtgcatcg aaagagcgca ggctggcatc gaaagcacaa
    57241 aaatcaagcg tgaaggcgat gcgcggcaaa gtacgcagcg gtcgggaata aaaagaagga
    57301 atggatggtg aaaaaagcga tagtgacagc gatggctgta atcagcctct ttactctgat
    57361 gggatgtaat aatcgggccg aagtcgatac gctttctccg gcgcaggctg ccgaactgaa
    57421 accgatgccg caaagttggc gcggcgtgct accgtgtgcc gattgcgaag gaatcgaaat
    57481 ctctctgttc ctcgaaaaag acggaacatg ggtgatgaat gagcgttatc tcggtgctcg
    57541 tgaagaacct tcctccttcg cttcctacgg tacatgggcg cgaaccgctg acaagctggt
    57601 attaaccgac agcaaaggtg aaaagtcata ttatcgggcg aaaggcgatg cgctggagat
    57661 gctcgatcgt gaaggcaatc caattgaatc gcagttcaac tatacgctgg aaccggcaca
    57721 atccagttta cctatgacgc cgatgaccct gcgaggcatg tatttttata tggctgatgc
    57781 ggcgaccttc actgattgcg cgaccggaaa acgtttcatg gtagcgaata acgcagagct
    57841 ggagcgtagc tacctggctg cgcgcggtca cagtgaaaaa ccggtgttac tgtcagtaga
    57901 aggtcacttt acgcttgagg ctaatccgga tactggtgcg ccaaccaaag tattagcgcc
    57961 cgatacggcg ggtaaatttt acccgaataa ggattgcagt agtttggggc agtaaacctg
    58021 cttaaggcag aaacaaacgc aaaattgcct gatgcgctgc gcttatcagg cctacagcat
    58081 ataatgcaac attttgaatt agcacgattt tgtaggccgg ataaggcgtt tacgccgcat
    58141 ccggcatttt gtgcacgagg ctggctttat cactgagtca gcccaacaaa gcgacccgcc
    58201 ttgatataaa tcccaggctt caggtgccca acatactgta actgcgtttc tggcttaaac
    58261 gcagagacat caccaatacg catatgcatc agatcggcag ggctaatcca gcccgattgc
    58321 gccagtgtaa gtggatgccc ggctttggca aatgcatcag tgacaaattc cgaacaaaac
    58381 cacgactttt tgtctccttc gcacacactg cttaactgcg ctttcgccag gtcgctgacg
    58441 cactgttggc gaaaatcctc ggagaacgga ttcagtgagc acatctggcg agtcaccata
    58501 aagggaataa attcgacaat gctgcgatag ttataaccgc tatctttgat tttattggca
    58561 aacgcggtga tatctgtggc ttgttgcggg gtaagatccg ggactcgtaa gacgaaaagc
    58621 ttatcactat gcttcatcgc ttttttaagg gaaacaatct ggacgccagc gcctgtcgct
    58681 tctgcaacgt tattatcacc cagaaagatt gcaacgtgac tcacagagga agtgctgaag
    58741 acgcggattc caaatgaggt taccccaagg cttgaggaga acagcaaatc gccgggtttg
    58801 agatcaggtg ccgttatttc ttttattgat tgttcggtga aagagctttg atgctggaat
    58861 ttgacagccc atgtttttgc ctcggcatct actgctgtgg ctgatgggtc tggctggctg
    58921 atatcaacgg tacaagccga aagtaaaaga aagctgggga gaagcaggcg gcagtacgcc
    58981 tttggtttat ccattttata caatccatgt aaaaaagggc cctgaaattc aggacccttt
    59041 ctggcatcag cctttaatct gtttcaccag atattcgacg atgtcaccag tcttaattaa
    59101 ctgtttctcg ccgttgcgac gatatttata ttcgatatcg tcgttgtcga ggttacggtc
    59161 gcccagcaca atagtgtgcg gaataccgat cagttccata tcagcaaaca tcacgcccgg
    59221 acgctctttg cggtcatcca gcagcacttc gataccttgt gcacgcagtt cgctgtacag
    59281 tttctcagca agctcttgta cgcggaagga tttgtgcatg ttcatcggca gaatcgccac
    59341 ctggaacggc gcgatagcgt caggccatac gatgccgcgt tcgtcgtagt tctgctcaat
    59401 cgccgcagct accacacgcg ttaccccgat accgtagcaa cccatcgtca ggatttggtt
    59461 acggccatct tcaccctgta cggaggcttt cagtgcttcg gagtacttgg tacccagctg
    59521 gaagatgtga ccaacttcga taccacgttt gatcagcagc gtaccctggc catccgggct
    59581 tggatcgcca gccaccacgt tacggatatc tgcaacttcc ggggtagcga catcgcgatc
    59641 ccagttgata ccgaagtagt gtttaccatc gatgttagca ccagcagcga aatcactcat
    59701 cgccgcaacg gtacggtcaa tcaccaccgg aatcggcatg tttaccggac ccagtgaacc
    59761 cggaccggct ttaaccacag cacgaatttc ttcttcggtc gcgaaagtca gcgggcttgc
    59821 aacctgcggc agtttttctg ctttaacttc gttcagctcg tgatcaccgc gcaccagcag
    59881 cgcaaccagc gggaagctac tgccttcaac cgctttaacc agcagagtct taaccgtttt
    59941 ctcaatcggc agattgaact gttcaaccag ttccgcgatg gttttcgcgt tcggcgtatc
    60001 aaccagcgtc atttcctggg tagcagcagc gcgcggttct ttcggcgcga tagcttctgc
    60061 cagttcaatg ttcgctgcat agtcagaggt gtcggagaag accacatcgt cttcaccgct
    60121 ctgcgccagc acctggaatt cgtgagaggc gctgccgccg atagaaccgg tgtcggcctg
    60181 tacggcgcgg aaatccagcc ccatgcggct gaagattttg ctgtaggccg catacattgc
    60241 atcgtaggtt tcctgcaggg attcctgaga agtatggaaa gagtaagcat ctttcatcag
    60301 gaattcgcgg gaacgcatga cgccgaaacg cggacgcact tcgtcgcgga acttggtctg
    60361 gatctgatag aagttcagcg gcagctgttt gtaagagcta agctcgttac gaatcaggtc
    60421 agtgataact tcttcatgag ttgggccgag tacgaacgga cgctcgccac ggtcaacaaa
    60481 acgcagcagt tccggaccgt actgttccca acgaccactc tcttgccaca aatcggctgg
    60541 ctgaaccacc ggcatcgaca cctcgatcgc accggcgttg ttcatctctt cacgcacgat
    60601 gttttcgact tttttcagaa cgcgcacgcc ggtcggcagc caggtatata acccggaggc
    60661 cagcttgcgg atcatcccgg cgcgcagcat cagctgatgg ctgatcacct cggcgtcggc
    60721 aggtgtctcc ttgagagtgg agagcaggta ttggctagta cgcatgttgt tacggttcca
    60781 gttggaaggt agaacaggct caaggcgagc ctgggacaaa aaaagtgatt tagtttacca
    60841 gtgcaaaaga aatgtcaaaa gagaagggcg tgaatttaac gcggttccag cgcaaagact
    60901 tcaaaacctg cgtcggtgac gcgccagcga acgttaaaat catgtagcca gacggcatag
    60961 gttttgcccg tttcctcacc tttacgatag gccgggcgcg ggtcctgcgc cagtacttcg
    61021 cggataaaca gcgttaactg cggataacgc ttctccagcg tcaaaagctg cttttcgacc
    61081 tctgcggtaa aactcaccgc catctctgca gctggcgcac tttgcgcata gctggcactg
    61141 gcatcgggaa gcgattcggc aaaggggaga tacggtttga tatccactac cggcgtacca
    61201 tcgaccagat ccagactgcc gagcttcaga atcacgctgt ctttatggca aacaacctct
    61261 ttcagctcta ccagcgacat gccaattggg ttagggcgga aagtagagcg tgttgcgaaa
    61321 acccccattc tggcgttacc gccgaggcgc ggtggacgca cagtcggacg ccagccgcct
    61381 tccatcgttt gatgaaagac gaaaaggatc cataaatggc tgaatgcttc caggccgcga
    61441 acggcgtcgg cctggttgta ggaagcaatg agatgcagtt cgccgttggc gctttttacc
    61501 agacctggct ggcgcggaac ggcgaatttt tctttatagg gcgagcgaat aacgcctatt
    61561 tgctcgaact ggaaactgct catttcgccg taatgttaag cgcagagccg atacatacag
    61621 cctgacgata gcagcccggc gtaccgctgg tgacttcgca gctatgcagt aatacagcat
    61681 tggctttcat tttagaggca ttgatttgca tctgcttacg tgcggttgga atgctcggcg
    61741 gagagtcctg attagaggcc tggcaagagt cgccactgac ttcaccgaga tcgcggaacg
    61801 gtttgccgac taattcttct gcattggtat aaattcggac cggcgtggcg cgcggcgctt
    61861 tcggttttgc aggctccgct ttcggctggg gtgcagtgct ttgaacgggt tcgacagggg
    61921 atctgcttaa catggaacag ccgcttagca tgagtgctac taaacagatc ggtaaagcac
    61981 gcatagtatt tcctcaatgt atgatcaaaa cgtcaatatt gaatcaggag cttgtaaaaa
    62041 tgacaagacg ggcaagcgcc cgtcctgaat gatattacaa attgtggaaa cagcctaaaa
    62101 attaccagcc tttaacagcg ccgccgttaa acactttgtt tgctgcttcg taaacttcat
    62161 cagactgata agcctggacg aatttcttca cgttctcggc gtctttgtta tcttcacgcg
    62221 ttacgatcag gtttacgtat ggggagtctt tatcttcgac aaagataccg tctttcgccg
    62281 gagtcaggcc aatctggctg gcataggtgg tattgataac tgccagagcg atttgcgcgt
    62341 cgtccagaga acgcggcagt tgcggtgctt ccagttcaac aattttcaga ttttttgggt
    62401 tctcaacaac atcaagaact gtcggcagca ggccaacgcc atctttcagt ttgatcaagc
    62461 ccactttttg cagcagcagc agtgaacgac caagattagt tggatcgttt ggcacggcaa
    62521 cctgcgaacc atcctgcagt tcatccagtg atttgatttt cttggagtaa ccagcaatcg
    62581 gataaacaaa agtgttgcct actgcgacca gtttgtagcc acgatctttc agttgctgat
    62641 caaggtacgg tttatgctgg aaggcgttgg cgtcgatatc gcctttgctc aatgcttcgt
    62701 ttggcagaac atagtcgttg aaggttacca gctcaacgtc caggccatat ttgtctttcg
    62761 caactttctg cgcgacttct gctacctgct gttcggcacc aacaatcacg ccgactttaa
    62821 tgtggtttgg atctttttca tcctgaccgc agcctaccag tgccagtgat ccaatcaggg
    62881 ctcccactgc cgcaaaggtt ttgaatttga acgccatacc ttattccttc ttcaattatt
    62941 tatgttgtgt tgaacgttac ttgcgagtga cagcccggac gatgcggtcg cctgcgaact
    63001 gaattaaata aaccagaatg accagcaata ccagtaccgt attcatcacc gtcgcgttgt
    63061 agccgatgta gccatactga tagccaatct gacctaaacc accggcaccg actgcaccac
    63121 ccatcgcgga ataaccgacc agggtaatca gggtgatagt tgccgcattc accagacccg
    63181 gcaacgcttc cggtaacagc actttacgga cgatctgcat tggcgtggca cccattgcgc
    63241 gggaagcttc aattaacccg gttgggatct ccagcagagc gttctcgacc atacgggcaa
    63301 taaacggtgc tgcaccaacg gttaacggaa caattgctgc ctgcaaacca atcgatgtac
    63361 cgacaataac gcgggtaaac ggaatcatcc ataccagcaa gataatgaac gggatggaac
    63421 ggaaaatgtt cacaatcgca gaaacggtac gatacagctt cgcgttagca ataatttgcc
    63481 ccggacgcgt gacataaagc agaacgccaa ccggcagacc aatcacaaac ccaaaaaatc
    63541 cggatacgaa ggtcattgcc agcgtttccc atacgccacg aaccagcagc cacatcatcg
    63601 gctcagacat aacccagtac ctctactttt acatggtgtt cctgcagcca ggcaatggcg
    63661 gcttgcgtat cttgttgtgt gccgtgcatt tcagtcagca tgatgccgaa tttaacgcca
    63721 ccggcgtagt ccatctgcgc gctaataatg ttgttgttga cgttgaaacg acgcgcggtt
    63781 tcagaaagca gtggggcatc gaccgattga ccggtaaact ccagacgcag catcggcacg
    63841 cagtcagtaa atggctccgt ttgcagacgt tcctggtaat cttccgggat atccagatgc
    63901 agggtcgact gaataaactt ctgcgccagc ggcgttttcg gatgcgagaa cacttcactt
    63961 accgtgtcct gctcgatcag ttcgccattg ctgatgaccg ccacgcaatc acaaatacgc
    64021 ttcacaacgt ccatttcgtg ggtgatcaac agaatcgtca aacccagacg gcggttaatg
    64081 tctttcagca gttcgagaat agaacgtgtc gttgccgggt ccagcgcgct ggtggcttca
    64141 tcacacagca atactttggg attgctggct aatgcacggg caatcgccac acgttgtttc
    64201 tgcccaccgg aaagattcga cgggtagcta tcatgcttat cgccaagacc aaccaatgac
    64261 agcaattccg tcacgcgacg tttgatctcg tctttcggtg tgttgtccag ctccagcggc
    64321 agagccacgt tgccaaaaac agtacgcgaa gagagcaggt taaaatgctg gaaaatcata
    64381 ccaatctggc ggcgagcttt ggtcaactcg gattctgaca gcgtggtcaa ttcctggcca
    64441 tcgaccagca cgctaccctc ggttgggcgc tccagcaggt ttacacaacg tataagcgta
    64501 ctcttacccg cgcctgaggc accgataacg ccataaattt gtccagctgg cacatgcagg
    64561 ctgacgttgt tcaacgcctg gatggtgcgg gtgccctggt ggaacacttt ggtgatattc
    64621 gaaagtttta tcattgatta tttattatcg tcattaagtt agtcgtagca tctcgaatgc
    64681 ctgaaacggg caacgccgtc aatgaaatgg atgttaaggc atccagacgt ctaaatcaat
    64741 caggtttatg cgaagagcac tttcttgcag gtcgaaacat gcgatactag cgtcccatgc
    64801 cttattaagg agctataaaa ggtggcgaag agcgtacccg caatttttct tgaccgtgat
    64861 ggcaccatta atgtcgatca cggctatgtc catgagatcg acaactttga atttatcgac
    64921 ggtgttattg acgccatgcg cgagctaaaa aaaatgggct ttgcgctggt ggtagtaacc
    64981 aaccagtctg gcattgctcg cggtaaattt accgaagcac agtttgaaac gctgaccgag
    65041 tggatggact ggtcgctggc ggaccgagat gtcgatctgg atggtatcta ttattgcccg
    65101 catcatccgc aggggagtgt tgaagagttt cgccaggtct gcgattgccg caaaccacat
    65161 ccggggatgc ttttgtcagc acgcgattat ttgcatattg atatggccgc ttcttatatg
    65221 gtgggcgata aattagaaga tatgcaggca gcggttgcgg cgaacgtggg aacaaaagtg
    65281 ctggtgcgta cgggtaaacc tattacacct gaagcagaaa acgcggcaga ttgggtgtta
    65341 aatagcctgg cagacctgcc gcaagcgata aaaaagcagc aaaaaccggc gcaatgatta
    65401 aaagatgaac ggttgaaata aaaatgcatt tttccgcttg tcttcctgag ccgactccct
    65461 ataatgcgcc t
//
LOCUS       DAETPZ010000011        65142 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.011, whole genome shotgun sequence.
ACCESSION   DAETPZ010000011 DAETPZ010000000
VERSION     DAETPZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.011"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="K0W59_000809"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="K0W59_000809"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBI3021957.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="K0W59_000810"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="K0W59_000810"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBI3021958.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="K0W59_000811"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="K0W59_000811"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBI3021959.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="K0W59_000812"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="K0W59_000812"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HBI3021960.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPTRGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="K0W59_000813"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="K0W59_000813"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HBI3021961.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLHQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="K0W59_000814"
     CDS             7826..9559
                     /locus_tag="K0W59_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HBI3021962.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="K0W59_000815"
     CDS             9708..10559
                     /locus_tag="K0W59_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3021963.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLRGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="K0W59_000816"
     CDS             complement(10546..10887)
                     /locus_tag="K0W59_000816"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBI3021964.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="K0W59_000817"
     CDS             complement(10889..11767)
                     /locus_tag="K0W59_000817"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HBI3021965.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCSWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="K0W59_000818"
     CDS             complement(11733..14030)
                     /locus_tag="K0W59_000818"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBI3021966.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQGRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGHDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="K0W59_000819"
     CDS             complement(14081..14401)
                     /locus_tag="K0W59_000819"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBI3021967.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="K0W59_000820"
     CDS             complement(14416..15495)
                     /locus_tag="K0W59_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBI3021968.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..18305
                     /gene="ptsP"
                     /locus_tag="K0W59_000821"
     CDS             15804..18305
                     /gene="ptsP"
                     /locus_tag="K0W59_000821"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBI3021969.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="K0W59_000822"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="K0W59_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20082
                     /gene="gldA"
                     /locus_tag="K0W59_000823"
                     /pseudo
     CDS             18990..20082
                     /gene="gldA"
                     /locus_tag="K0W59_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20357..20974
                     /locus_tag="K0W59_000824"
     CDS             20357..20974
                     /locus_tag="K0W59_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HBI3021970.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPDGDVPQECGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21001..21906)
                     /gene="yijE"
                     /locus_tag="K0W59_000825"
     CDS             complement(21001..21906)
                     /gene="yijE"
                     /locus_tag="K0W59_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HBI3021971.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22000..24180)
                     /gene="katG"
                     /locus_tag="K0W59_000826"
     CDS             complement(22000..24180)
                     /gene="katG"
                     /locus_tag="K0W59_000826"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HBI3021972.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGVNGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24509..25399)
                     /gene="metF"
                     /locus_tag="K0W59_000827"
     CDS             complement(24509..25399)
                     /gene="metF"
                     /locus_tag="K0W59_000827"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBI3021973.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGICHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25748..28180)
                     /gene="metL"
                     /locus_tag="K0W59_000828"
     CDS             complement(25748..28180)
                     /gene="metL"
                     /locus_tag="K0W59_000828"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HBI3021974.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28183..29343)
                     /gene="metB"
                     /locus_tag="K0W59_000829"
     CDS             complement(28183..29343)
                     /gene="metB"
                     /locus_tag="K0W59_000829"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBI3021975.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29620..29937
                     /gene="metJ"
                     /locus_tag="K0W59_000830"
     CDS             29620..29937
                     /gene="metJ"
                     /locus_tag="K0W59_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HBI3021976.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30121..30729
                     /locus_tag="K0W59_000831"
     CDS             30121..30729
                     /locus_tag="K0W59_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HBI3021977.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30790..31002)
                     /gene="rpmE"
                     /locus_tag="K0W59_000832"
     CDS             complement(30790..31002)
                     /gene="rpmE"
                     /locus_tag="K0W59_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBI3021978.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31205..33403
                     /gene="priA"
                     /locus_tag="K0W59_000833"
     CDS             31205..33403
                     /gene="priA"
                     /locus_tag="K0W59_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBI3021979.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33559..34584
                     /gene="cytR"
                     /locus_tag="K0W59_000834"
     CDS             33559..34584
                     /gene="cytR"
                     /locus_tag="K0W59_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HBI3021980.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34676..35635
                     /gene="ftsN"
                     /locus_tag="K0W59_000835"
     CDS             34676..35635
                     /gene="ftsN"
                     /locus_tag="K0W59_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HBI3021981.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35728..36258
                     /gene="hslV"
                     /locus_tag="K0W59_000836"
     CDS             35728..36258
                     /gene="hslV"
                     /locus_tag="K0W59_000836"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HBI3021982.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36268..37599
                     /gene="hslU"
                     /locus_tag="K0W59_000837"
     CDS             36268..37599
                     /gene="hslU"
                     /locus_tag="K0W59_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HBI3021983.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37666..38592
                     /gene="menA"
                     /locus_tag="K0W59_000838"
     CDS             37666..38592
                     /gene="menA"
                     /locus_tag="K0W59_000838"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBI3021984.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38685..39170
                     /gene="rraA"
                     /locus_tag="K0W59_000839"
     CDS             38685..39170
                     /gene="rraA"
                     /locus_tag="K0W59_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HBI3021985.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39255..39500)
                     /gene="zapB"
                     /locus_tag="K0W59_000840"
     CDS             complement(39255..39500)
                     /gene="zapB"
                     /locus_tag="K0W59_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HBI3021986.1"
                     /translation="MTMSLEVFDKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39926..40771
                     /gene="glpF"
                     /locus_tag="K0W59_000841"
                     /pseudo
     CDS             39926..40771
                     /gene="glpF"
                     /locus_tag="K0W59_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40811..42301
                     /gene="glpK"
                     /locus_tag="K0W59_000842"
     CDS             40811..42301
                     /gene="glpK"
                     /locus_tag="K0W59_000842"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBI3021987.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYISSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGTSIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLSAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42531..43541
                     /gene="glpX"
                     /locus_tag="K0W59_000843"
     CDS             42531..43541
                     /gene="glpX"
                     /locus_tag="K0W59_000843"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBI3021988.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43638..44384
                     /gene="fpr"
                     /locus_tag="K0W59_000844"
     CDS             43638..44384
                     /gene="fpr"
                     /locus_tag="K0W59_000844"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HBI3021989.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44389..44817)
                     /gene="uspD"
                     /locus_tag="K0W59_000845"
     CDS             complement(44389..44817)
                     /gene="uspD"
                     /locus_tag="K0W59_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HBI3021990.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44844..45143)
                     /locus_tag="K0W59_000846"
     CDS             complement(44844..45143)
                     /locus_tag="K0W59_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HBI3021991.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAAFSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45355..45795)
                     /locus_tag="K0W59_000847"
                     /pseudo
     CDS             complement(45355..45795)
                     /locus_tag="K0W59_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45896..46495
                     /locus_tag="K0W59_000848"
     CDS             45896..46495
                     /locus_tag="K0W59_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HBI3021992.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46603..47370
                     /gene="tpiA"
                     /locus_tag="K0W59_000849"
     CDS             46603..47370
                     /gene="tpiA"
                     /locus_tag="K0W59_000849"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBI3021993.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47425..48180)
                     /gene="cdh"
                     /locus_tag="K0W59_000850"
     CDS             complement(47425..48180)
                     /gene="cdh"
                     /locus_tag="K0W59_000850"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HBI3021994.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMSTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48287..49276)
                     /gene="sbp"
                     /locus_tag="K0W59_000851"
     CDS             complement(48287..49276)
                     /gene="sbp"
                     /locus_tag="K0W59_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HBI3021995.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49596..50558)
                     /gene="pfkA"
                     /locus_tag="K0W59_000852"
     CDS             complement(49596..50558)
                     /gene="pfkA"
                     /locus_tag="K0W59_000852"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBI3021996.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50739..51641)
                     /gene="fieF"
                     /locus_tag="K0W59_000853"
     CDS             complement(50739..51641)
                     /gene="fieF"
                     /locus_tag="K0W59_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HBI3021997.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51789..52289)
                     /gene="cpxP"
                     /locus_tag="K0W59_000854"
     CDS             complement(51789..52289)
                     /gene="cpxP"
                     /locus_tag="K0W59_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HBI3021998.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52439..53137
                     /gene="cpxR"
                     /locus_tag="K0W59_000855"
     CDS             52439..53137
                     /gene="cpxR"
                     /locus_tag="K0W59_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HBI3021999.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53134..54507
                     /gene="cpxA"
                     /locus_tag="K0W59_000856"
     CDS             53134..54507
                     /gene="cpxA"
                     /locus_tag="K0W59_000856"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HBI3022000.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTKLLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54613..55287)
                     /gene="yiiM"
                     /locus_tag="K0W59_000857"
     CDS             complement(54613..55287)
                     /gene="yiiM"
                     /locus_tag="K0W59_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HBI3022001.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(55436..56419)
                     /gene="kdgT"
                     /locus_tag="K0W59_000858"
     CDS             complement(55436..56419)
                     /gene="kdgT"
                     /locus_tag="K0W59_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HBI3022002.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAIIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(56679..57299)
                     /gene="sodA"
                     /locus_tag="K0W59_000859"
     CDS             complement(56679..57299)
                     /gene="sodA"
                     /locus_tag="K0W59_000859"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HBI3022003.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            57584..58618
                     /gene="rhaT"
                     /locus_tag="K0W59_000860"
                     /pseudo
     CDS             57584..58618
                     /gene="rhaT"
                     /locus_tag="K0W59_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(58587..59480)
                     /gene="rhaR"
                     /locus_tag="K0W59_000861"
     CDS             complement(58587..59480)
                     /gene="rhaR"
                     /locus_tag="K0W59_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HBI3022004.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRISIRRKINSPCRCRRC"
     gene            complement(59536..60372)
                     /gene="rhaS"
                     /locus_tag="K0W59_000862"
                     /pseudo
     CDS             complement(59536..60372)
                     /gene="rhaS"
                     /locus_tag="K0W59_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
     gene            60660..62129
                     /gene="rhaB"
                     /locus_tag="K0W59_000863"
     CDS             60660..62129
                     /gene="rhaB"
                     /locus_tag="K0W59_000863"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBI3022005.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELITLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            62126..63385
                     /gene="rhaA"
                     /locus_tag="K0W59_000864"
     CDS             62126..63385
                     /gene="rhaA"
                     /locus_tag="K0W59_000864"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBI3022006.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            63627..64451
                     /gene="rhaD"
                     /locus_tag="K0W59_000865"
     CDS             63627..64451
                     /gene="rhaD"
                     /locus_tag="K0W59_000865"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBI3022007.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLAVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            64461..64775
                     /gene="rhaM"
                     /locus_tag="K0W59_000866"
     CDS             64461..64775
                     /gene="rhaM"
                     /locus_tag="K0W59_000866"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBI3022008.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            65076..>65142
                     /locus_tag="K0W59_000867"
     CDS             65076..>65142
                     /locus_tag="K0W59_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="with FrvBRX is part of the
                     phosphoenolpyruvate-dependent sugar phosphotransferase
                     system involved in the transport and phosphorylation of
                     sugars; possibly responsible for the sugar specificity of
                     the system; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBI3022009.1"
                     /translation="MAALTASCIDLNIQGNGAYSVL"
BASE COUNT        15882 a        17551 c        16665 g        15044 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gccaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccaacacg cggagttaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acacaaccag ctgatgcgtc
     5521 gcctgcacca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctaggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttcct ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atagcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgcgcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac attttgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgagcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc agcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cgtgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggccctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccaatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggagtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt cgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctggctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gccgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaag agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcctag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgtgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcagtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt taggttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctctaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctcac
    19741 gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca cggtgaaaaa
    19801 gtggcatttg gtacgctgac gcagctggta ctggaaaacg cgccggtgga ggaaatcgaa
    19861 accgtagctg cacttagcca tgcggtaggt ttgccaataa ctctcgctca actggatatt
    19921 aaagaagatg tcccggcgaa aatgcgcatt gttgcaaaag catcttgtgc agaaggtgaa
    19981 accatccaca acatgcctgg cggcgcgacg ccagatcagg tttacgccgc actgctggta
    20041 gctgaccagt acggtcagcg tttcctgcaa gagtgggaat aacctactcc aaactcccgg
    20101 cttgtccggg agtttgaacg caaaattgcc tgatgcgcta cgcttatcag gcctacgcaa
    20161 tctctgcaat atattgaatt tgcgtgcttt tgtaggccgg ataaggcgtt cactccgcat
    20221 ccggcactct gtgccagtaa tctgaaactc ccacgttgtt caggagtttt ttatccgctt
    20281 ctggacattt tctccacaga acaatcgtta gcccggaagg tcgaatctgt gactcttatt
    20341 ttcacggacg aaccagatga aagcttcact ggcgctgctc agcctgctaa cggcatttac
    20401 cagccactcc ttaaaatctc ccgccgttcc accaaccgtg gtacaaatcc aggccaatac
    20461 caaccttgcc atcgcagacg gtgccagaca gcaaattggc agcacgctat tttacgatcc
    20521 ggcgtatatg cagcttacct atcctgacgg tgatgttccg caagaatgcg gtgtatgttc
    20581 cgatgtggtg atccgcgcat tgcgcagcca gaaggtcgat ttgcagaaac tggttcacga
    20641 agatatggcg aagaattttg ctgagtaccc gcaaaaatgg aagttaaagc gcccggacag
    20701 caacatcgat caccgtcggg tgcctaatct ggaaacctgg tttagccgcc acaacaaaac
    20761 acgccccatc agtaaaaatg ccggtgacta tcaagcgggc gatattgtct cctggcgatt
    20821 agataacggg ctggcgcata ttggggtggc gtcagatggc ttcgcccgcg acggtacgcc
    20881 gctggtgata cataacattg gcgcaggcgc gcaggaagaa gatgtgctgt ttagctggcg
    20941 gatggtgggg cattatcgtt attttgttaa ataagagatc gccccacatg aagaaaaaat
    21001 tcagatgctt tttacactga cagcttcttt tttcttacgg ctcaccagcg ccagtgccag
    21061 cacaatcagc acaataccgc taccttcaac ggcccccgga ttctcaccga gcagccacca
    21121 ggaaaagagt acgccacaaa cgggaacggc cagtgtgctt aagctggcaa tactggcagg
    21181 caagtttttc agcacaaaca gccataagct ccacgctagc gccgtcgcca gaatcgcact
    21241 gtaggccagc gcccagaaca cggtgggctg ccagtcaatt tcacgttgcg gcaccagtaa
    21301 agcgaccaca ctcatcacca gcgccgcata cagcatctgc caggatgtta acgataataa
    21361 atccacgcgc ggatgacggg cgtataggcg tttagcaaca atcgcgctcg ccccccaact
    21421 aacaccggag aggattgcca gcatggcact tttcatcgaa gagaaatcga gttgccacgg
    21481 ctgcaacacc aaaaataagc cgaaagcggc aatcagaatc gcgaaatatt gcccacgtcg
    21541 caggcgttca ccgagaaaca acgcggcgaa aatcaccacc cagaacggca tggtatagct
    21601 caggatcgcc accttccccg cacctccgct gaccaacgcc cactgcgcca gaccaaccat
    21661 cccgcaggtt tgtaacaggg caatggctaa ggtgtattta aacggcgtcg ggcgcattcc
    21721 gcgaccacgt aataaaagga cgatgaataa aacgagagcg ccgaaaatgc agcgtaaggc
    21781 ggtaaagtcg aaggcaccga tgtaactggt gacttgcttc atgaaaatcc agctataact
    21841 ccagataagt gtgagcacaa ccaggccact gattgccagt gggttgctct ttcctgcggc
    21901 agacatgaga gatccagtgt gtagttttcc tgtggacact atacgccggt aaagaaactt
    21961 cagcgactgc ctgaaagaca gtcgctgaag aaggtcagat tacagcaggt cgaaacggtc
    22021 gaggttcatc actttcaccc atgccgctac gaagtcttta acaaacttct cgtgggcatc
    22081 gctgctggcg taaacttccg ctactgcacg caggacagag ttagaaccaa ataccagatc
    22141 cgcacggctg gctgtgtatt tcacttcgcc ggtttcgcgg tcacgtcctt cgaacagctc
    22201 tttcgattcg tcagtcgctt tccactcgta acgcatatcc agcaagttca cgaagaagtc
    22261 attgctcaat acgccaacgc ggtcagtgaa gacgccgttt ttgctgccat cgaagttggc
    22321 acccagtaca cgcatcccgc ccaccagcgc agtcatttcc ggcgcggtca gcgtcagttg
    22381 ctgtgctttg tcgatcagca gtgactcggt ggtggaaacg tccagacgag cgcgatagtt
    22441 acggaaaccg tcagcaattg gctccagcag ctcaaacatc tcaatgtcag tctgatcctg
    22501 acgcgcatca acgcgacccg gcgcaaacgg tacatgaatg ctcaaacctg cggcgcttgc
    22561 ggctttctca acaccaacca caccagccag cacgatgata tccgccagcg aggctttacc
    22621 agactctttc tggattttct ccagaacagg cagagcacga acggctgcgg cgttcacatc
    22681 ccagtcgcgc tgcggcatta atgccagacg cgcaccgttg acaccaccgc gtttgtcgcc
    22741 accacggaag gtagaagcag atgcccaggc caccgatacc agctcactaa cagacagacc
    22801 agaatccgca atcgcgaatt tcagatcgat aatgtcctgc tcggtcgggt tgtagatcgg
    22861 ctgcggcagc ggatcttgcc agatcagatc ttctttcggc acttccggcc cgatgtagcg
    22921 agatttcggc cccatatccc tgtgcgtcag tttgaaccag gcacgggcaa aggcttcgtt
    22981 gaacgcctgc ggatcgttga ggaaacgacg agagatcttc tcgaactcag gatcaaaacg
    23041 cagcgtcagg tcggtcacca gcattgtcgg tttacgtttc ttcgacggat cgaacggatc
    23101 cgggataatt tccggtgcgt ctaccgcttc gaactggatt gctccagccg gactgcgggt
    23161 ctgtacccac tcatacttga acagattctc gaagaaatag ttgctccact gggtcggcgt
    23221 ctgggtccag actacttcca gaccagaggt aatggcatct gcgccaacgc cgctgccgta
    23281 agtgctcgcc cagcctaaac cttgttcttc aatcggtgca gcttctggat caggacctac
    23341 gtttgatgtc ggaccggcac cgtgggtttt acccagcgta tgaccacccg caatcagcgc
    23401 cacggtttct tcgtcgttca tgcccatgtt gccgaaggtc gcgcggatag ctgctgccgc
    23461 agaaagcggt tcgccgctgt gatccgggcc ttccgggtta acgtaaatca ggcccatctc
    23521 ggttgcaccc agcggtgctt tcgccagcgc ttccggatga cggtgagtca gccaggcttt
    23581 ttcatcaccc cagttaacat ccagatccgg ttcccagacg tcttcacgac cggcaccaaa
    23641 accgaaggta cggaagccgg agttttccag cgccacgtta cctgcgagga taaacaggtc
    23701 ggcccaggag attttctgac catatttctg tttgattggc cacaacaggc gacgcgcttt
    23761 atcgaggctt acgttatccg gccaggagtt cagcggtgca aaacgttgct gaccacgacc
    23821 cgcgccgccg cgtccatcga ttgaacggta agtacctgcg ccgtgccaag ccatacgaat
    23881 aaacagaccg gcgtagctgc cccagtcggc tggccaccac ggctgagatt ctgtcagcag
    23941 ggctttcaga tcttttttca ggccgtagta atctaatttg ctgaattctt tgcggtagtc
    24001 aaagtcctca cccagcgggt tagaacgatt agaatgttgg tttaacaggt caacacggag
    24061 ttgatttggc caccagtcgc gagtggttgt gcccgccccc gcgctctggt cgtgaccgcc
    24121 ctgatggaac gggcatttgc cagtggctgt ggtgttatgg atatcgtctg acgtgctcat
    24181 caatgtgctc ccctctacag tgttaccgtt acgatacaca gcgttagaga gaagttataa
    24241 ttgaattaat ccacggatgc gatagttgag atcttacata ttgttggtta aagagatgta
    24301 gatcaaattg atcgtaatta agcttttttg attatgtccg aattcggaca aatgctttat
    24361 aaaaaagggt tttagcttca tatccttcaa gtaatgaggg agaaaacaag gctgacaacc
    24421 ttattttccc gccctcattt cgaggcagca ttttgtgctc tgtttaaaat ttgtgatcac
    24481 tgtgtgattt tcacaaaagc cacactattt ataaaccagg tcgaacaccc agcgtatggc
    24541 aaatcgcgta actcatttca gcgcggttaa gcgtatagaa gtggaaatct ttcactcctt
    24601 cacggcttaa aatcttcacc atatccatgg caatattcgc gcccaccagt ttgcgggttt
    24661 cggcatcgtc atccagcccg tcaaacattt gcgccatcca cgccggaata cgcacgttgg
    24721 tcatgtcggc aaatttcttc gcctgtttaa agttagatac cggcaaaatg cccgggatga
    24781 tttccacatc aatgcccgcc gatacacagc ggtcacgaaa acgcaggtag ctttcgacat
    24841 cgaagaagaa ctgagtaatc gcgcggttgg ctccggcatc cactttgcgt ttcagattaa
    24901 gcaaatccgc ctgagcgctt tttgcttccg ggtgaacttc cggatacgcc gccacggata
    24961 tatcgaaatc tgccacttct tttaacagcg tcaccaggtc agaagcatac atttctggct
    25021 taccacttcc cggcggcaga tcgccacgca gtgccacgat atgacaaata ccgttattcc
    25081 agtagtcgcg tgcaatggtg cgcagctcgt cgggcgtcgc atcaatgcaa gtaagatgcg
    25141 gtgccgcttc cagaccagtg cgatctttaa tgcctttaat aatgctgtgc gtacggtcgc
    25201 gctcgccgga gttcgcgcca taggtcaccg atacaaactt cggtttcagg ctgctaaggc
    25261 gatcgatgga gttccacagg gtctgctcca tttcactggt acgcggcggg aaaaactcga
    25321 acgaaacgtt aatctgcccc tggacttctg ccaggctctg attcagggca tcccgctggc
    25381 tggcgtgaaa aaagctcata ccttacctca tcaatcgctt gtagttatat gttgtgttgt
    25441 gcacatctat ccatttagac gtccagatgt aaagatgaag gaaaagccga agggcgtcaa
    25501 ccgaaaaatg accaatgatg gtgaggaaaa atcagggaag atgaaaaaac ttcaggatgg
    25561 taagaaaaag aaaatccgat atgcaaagca aaccggactg caaatataaa ctaaccatgt
    25621 acttattgct tcttgccgga ttcgtcgtaa acgcttcatc cggcctaatt ctatgataat
    25681 tcaatatatt tcattagaaa ccatagcccc gaaaacatga gtaccgggca ttattaaatt
    25741 tctgaaatta caacaactgt gccagccggt tgatatccga ctgaatcgcc ccggcggtga
    25801 cgtcgcgccc agcgccaggc ccacggatca ccagcggatt atcgcggtac cagcggcttt
    25861 cgatggcaaa gacgttatcg catggcagca gtgatgccag cggatggtct tcacgcaccg
    25921 cctccacgcc cacacgcgct ttgccgttgg catcgaaacg cgccacgtag cgcagcacca
    25981 gccccatttc gcgggccgct tccagccgtt gcaccatctg ctcgttaagt tcatcgccat
    26041 tttcaaagaa atggtcgatg ctgccacctt cgcaatgagc aggcaccagc gattccacac
    26101 gtacctggtc cggttcaatg ttgtaacccg cttcacgcgc cagaatcacc agcttgcgca
    26161 ttacgtcttt gccggagagg tcgtcacgcg gatcaggctc ggttaagccc tgctgccacg
    26221 cctgatccac cagctcggta aacggcacgc taccgtcgaa ttgcaggaac agccaggaga
    26281 gcgtgccgga gaagatccca ctgatcgaca aaatggtatc gccgctgtcg atcagatcgc
    26341 gcacggtatg gttgatcggc aagcccgcac caacggtggc gttgtatagc cagtgacgcc
    26401 cggttttttc gaaggcgtcg tggatctggc gatatttatt gctgtcgctt gctcccgcca
    26461 gtttgttggc actgatgacg tggaaaccgt ggctggcgaa atcgagatac tgatcagcaa
    26521 gctgctggct ggcggtaacg tccaacacca ctaaatcatc atacggatgg gcgcgcatcc
    26581 acaggaaaag cgactcttca tcctgctcga ccgcttcatc gttgaagaag gctaacgcgc
    26641 ggctggcgtc cagcccgtca taactcaaca gactgcggcg gctgtccacc acgcctgcca
    26701 gcacaaattc aaagccagta cgcgccgaaa gcgtgctctg ctcgcgggcg aacagttcca
    26761 gccagcgaga gccgatattg cctttgccga acaataccag gccgatgcgt ttttctgcgc
    26821 ggaagacgga ctgatgcagc ccctgaatca ggctttcggt cgggccggtg cgcagtactg
    26881 ccaccagact gatgccgtca tcggactgcc acgtaaactc gaccggctgg cctttcagtt
    26941 gctgccagaa gcggtggcaa tgcagcgggt tacgggtgac acccgccccg accatcgcca
    27001 ccagcgccag cccctgacgc aggcgcagtt ctccaggtaa tcccgcttcg tcgaggattt
    27061 tcagcgcact gtcggccact tctgaggtgt agcaaaattg cagcaactgg cgatcgttat
    27121 gcacgccaac cgccagtgga cgcacctggg cacgtttcag gatctggtcg atctctttat
    27181 gcgccagttt gaaatcctga ctggcgggca cctgaaactc aatcaaacag acatcatcgt
    27241 ggctggtgac aatacgcgca ccagtaccgg aggccagcac gcgttcaatg cgcgtggaac
    27301 cttgatccgg cgtgtagcta cagcgcagct gcaggtcgat ttcgctgcca gaaactggct
    27361 gtaaagtacg ggcgtgaaga acgggagccg ccagacgcgc cagttcgctg gcctcatcca
    27421 gacgcagcaa cggcagcagg caggcatctt tcactttacg cgggtcggca ctgtataccc
    27481 cggcgacgtc gctccagatg gttacacgag aaacacccgc cagcgcaccg atttgtgtcg
    27541 cggaatagtc ggaaccgtta cgccccagca acaccgtttc accggcgttg ttacggctga
    27601 taaacccggt caccaccaga cgtttgctcg gatgttgtac cagtagctgt tgcagcaacg
    27661 ggtaagaaag cccttcatca acctgcggtt gcgcagcgcg ttcagcgcgt aaaaactcac
    27721 gggcatcaag ccaggccgct ggcagccctt gttgattaag taccgcagac atcaggcgcg
    27781 ccgaccacac ttcaccgtgg cccaccactt ccgcatacac cgcatcgtta ataccactgt
    27841 cgagcaacgc cgccaggcgc tcaagatcgc tgacaaaagc gctaatgagg ctatcggctt
    27901 cttcagcggg tagcagaccg ctaatcagat cgcactgata gcgacgcagc gtttgttgaa
    27961 cctgatgcgc agagagacga tcggtctggc ttagtttcaa ccagttaatc aactggttag
    28021 tggtgctacc ggcggcggaa accaccatca tatcgtcagg ctgagagaac tccgccataa
    28081 tgcccgcgac acgcaaataa cacttcacat cagccagact actgccacca aatttatgca
    28141 gctgacgacc tttcgcccct gcctgcgcaa tcacactcat ttttacccct tgtttgcagc
    28201 ccggaagcca ttttccaggt cggcaattaa atcttcgcca tcttcaatac cggtggagat
    28261 acgcagcagc gtctcggaga tcccggcggc agcacgcgct tctggtgcca tgcctgcatg
    28321 tgtcatggtt gcggcgtgag agattaaact ttccactccc cctaatgatt ccgccagcgt
    28381 aaacaacgac agcccgccca ggaaacgaca cagcgtctgc tcatcgccat ccagttcaaa
    28441 actcaacatt gcgccaaagc ctttttgctg acgcgcggca atttcatgcc cctgattttc
    28501 cggcagcgac gggtgataca gttttttcac caacggctgg gtttgcagat atttcacaat
    28561 cgcctgcgcg ttgcgctgcg ccagctccat acgcggcacc agcgttcgca acccgcgtag
    28621 cagcagatag ctgtcaaacg cgccgcccgt cacgccaata ttgtttgccc accaggcaag
    28681 ttcagtgaca acgtccgggt ctttagcaat caccacgcca gccaccacgt cagagtgacc
    28741 gttcagatat ttcgtacatg aatgcaacac cagatcggca cccaatgcca gcggattttg
    28801 taatgccggg cttaagaagg tgttatccac cacgctcacc gccccgactt cccttgccag
    28861 atggcagatt ttcgcaatat ccacgacgcg taacaatgga ttacttgggc tttctaccag
    28921 taccagtttg ggtttttctg ccagcgctgc ccgtaatgcc tgttcatcgc cttgatcaac
    28981 aaacaacacg cgatagcaac cgcgtttcgc cagactgtcg aacaggcgat agctaccgcc
    29041 gtagcagtcg tgcggcgcaa ccagcagatc gccaggtttc aaaaagacgg tcgttaccag
    29101 gtgaatcgcg gacatgccgg tattagtaag tactgcgcca gcaccgcctt ccagttctgc
    29161 cagcgcacgc tgaaccacat cgcgcgttgg gttgccgcga cgcgagtaat catgcgcgcg
    29221 cggttcatta aatccggtaa agttataggt gctggaaaga tggatgggag ggacaacgca
    29281 accatactgt tcgtcgtcat ttaacccgct acgcactgcg atggtggcct gtttacgcgt
    29341 catgtgatga agtttcctgg gctttgtcgg tgaaatgtca ggcaccagag taaacattgt
    29401 gttaatggac gtcaatacat ctggacatct aaacttcttt gcgtatagat tgagcaaatc
    29461 ccaaatagcc gttaaaatta tatgcattat cacgccgaca ggtgcattac acgatgtcac
    29521 ggtaacgcct gtacggtaaa ctatgcgggt ttacggtcag taccaacata aattgtgggg
    29581 tctggtctca atttattgac gaagaggatt aagtatctca tggctgaatg gagcggcgaa
    29641 tatatcagcc catacgctga gcacggcaag aagagtgaac aagtcaaaaa gattacggtt
    29701 tccattcctc ttaaggtgtt aaaaatcctc accgatgaac gcacgcgtcg tcaggtgaac
    29761 aacctgcgtc acgctaccaa cagcgagctg ctgtgcgaag cgtttctgca tgcctttacc
    29821 gggcaacctt tgccggatga tgccgatctg cgtaaagagc gcagcgacga aatcccggaa
    29881 gcggcaaaag agatcatgcg tgagatgggg attaacccgg agacgtggga atactaaagc
    29941 ggaaaagagc ggcgcggagt ggaatcgcct gatgcactac gcttatcagg cctacgtcat
    30001 attgcaattt attgaatttg cacgaacttg taggccggat aaggcgttca cgccgcatcc
    30061 ggcataaaca acgagcacgt tgtctgcgac ccaccgcttt ttatacatgg acgtttaact
    30121 atgaaaaaca ggctgctgat cctcagcctg ctggtttctg tacctgcctt tgcctggcag
    30181 ccgcaaactg gcgacatcat ctttcaaatc tctcgctcat cgcaaagtaa agcgatccaa
    30241 ctggcaaccc attccgatta cagccacacc ggtatgctgg tgatgcgtaa caaaaagccc
    30301 tacgtttttg aagcggtcgg cccggtgaaa tacaccccgc tcaagcagtg gattgcccat
    30361 ggtgaaaagg gcaactacgt tgttcgccgc gttgaaggcg gactgagtgt tgaacaacag
    30421 caaaaactga cgcaaacggc aaaacgttat ctcggtaaac cgtatgattt cagcttctca
    30481 tggagcgacg atcgccagta ctgttcggaa gtggtgtgga aggtttacca gaacgcgctg
    30541 ggaatgcgcg tgggcgaaca gcagaagttg aaagagtttg atctcagcaa cccgctggtc
    30601 caggcgaagc tcaaagaacg ctacggtaaa aatattccgc ttgaggaaac ggtagtttca
    30661 ccacaagccg ttttcgacgc gccacaactc accacggttg ccaaagagtg gccgctgttt
    30721 tcgtggtgat acgaccaaaa ttcaggcaca aaaaaagcgc cgtgcggcgc ttttttcgga
    30781 aatccggtct tatttgctgc ccgggatgtt gaaacgcttg ttgaagcggt caacacggcc
    30841 accggtagca acatcacgct gtttgccggt gaagaacggg tggcacttgc tgcacacgtc
    30901 gaggttcagg tcatgaccaa cggtggagcg gattttcatt acgttaccgc aagagcagct
    30961 agcagtaatt tcttcgtatt tcgggtgaat atcttttttc atgggaaaac ctcggtttaa
    31021 ggccgcgtcg ctcttccagc cctaacgcca gacaccacgc gatgttaaaa gtatagcttc
    31081 aatacgatca tttcgtacga aggcgcgaaa tcatacagaa attaaccagc gtatgcaaac
    31141 tgatccgcac tcttctacgg caatgtgtat actaacccac cgaatttcaa gtcaggatga
    31201 tgctatgccc gttgcccacg ttgccttgcc cgttccgctt cctcgtacct ttgactatct
    31261 gctgccagaa ggcatgacgg ttaaagctgg gtgtcgcgtg cgcgtgccgt ttggcaaaca
    31321 gcaggagcgc atcgggattg tggtatcagt tagcgatgcc agcgaactgc cgctcactga
    31381 gctaaaagcg gtagtcgaag tgctggatgg tgagccggtg tttactcact ccgtctggcg
    31441 attgctgcta tgggcggcag attactatca tcatccgatt ggcgatgtgc tgtttcatgc
    31501 cttgccgatt ttactacgcc aggggcggcc tgcggcgaac gcgccgatgt ggtactggtt
    31561 tgccactgaa caaggtcagg cggtggatct gaacagcctg aaacgctcac ccaagcaaca
    31621 acaggcgctg gcagcgttac ggcaaggcaa aatctggcgc gaccaggttg ccacgctcga
    31681 atttaatgat gccgcgttgc aggcgctgcg caaaaaaggt ctgtgtgatt tagcaagtga
    31741 aacaccagag tttagcgact ggcgaacgaa ctatgccgtt tctggtgagc ggttgcgatt
    31801 gaataccgaa caggccaccg ccgttggcgc aattcatagc gcggcagata ctttttctgc
    31861 ctggctgctg gcgggcgtta ccggttccgg taaaacggag gtttatctca gtgtactgga
    31921 aaacgtgctc gctcagggca aacaggcgct ggtgatggtg ccggaaatcg gcctgacacc
    31981 gcaaactatc gcccgttttc gtgaacggtt taatgcgccc gtggaagttc tgcattccgg
    32041 tctgaacgac agcgagcgtc tttcggcgtg gctgaaagcg aaaaatggcg aggcggcgat
    32101 tgtgatcggc acccgctccg cgctgtttac gccgtttaaa aatctcggcg tgattgtcat
    32161 tgacgaagag cacgacagtt cctacaagca gcaggaaggc tggcgctatc atgcccgcga
    32221 cctggcggtg tatcgtgcgc atagcgagca aatcccgatt attcttggct ccgcaacgcc
    32281 cgcgctggaa acgttgtgca acgtccagca gaaaaaatac cgcctgctgc gcctgacgcg
    32341 ccgggcgggg aatgcgcgtc cggcaattca acacgtgctg gatttaaaag gtcagaaggt
    32401 gcaggcaggt ctggctccgg cgttaatcac ccgtatgcgc cagcatttac aggccaacaa
    32461 tcaggttatc ctctttctta accgccgtgg ctttgcgcct gcgctactgt gccacgactg
    32521 cggctggatt gccgaatgcc cacgttgcga tcactactac acgctgcatc aggcgcaaca
    32581 gcatttgcgc tgtcaccact gtgacagtca gcgtccggtg ccgcgccagt gcccttcctg
    32641 cggttccacg cacctggtac ccgtggggct gggcaccgaa cagcttgaac agacgcttgc
    32701 gccgctgttc cctgatgtgc ccatttctcg tatcgaccgc gataccacca gccgcaaagg
    32761 ggcgctggaa cagcaactgg cggaagtaca tcgcggcggt gcgaggattt tgatcggcac
    32821 acaaatgctg gcgaaaggtc accactttcc ggatgtgacg ctggtggcgt tactggatgt
    32881 ggacggcgcg ctgttttctg ccgattttcg ctcggcagag cgtttcgctc agctttacac
    32941 ccaggtcgct ggtcgtgccg ggcgtgcggg taaacaaggc gaagtggtgc tgcaaacgca
    33001 ccatccggaa catcctctgt tgcaaacgtt gctctataaa ggctacgacg cctttgccga
    33061 acaggcgctg gctgagcgac ggatgatgca actgccgcca tggaccagcc atgtgattgt
    33121 gcgtgcggaa gatcataaca atcagcatgc gccgctgttc ctgcaacaac tgcgtaatct
    33181 gatcctctcc agtccgctgg cagacgacaa actgtgggtt ctcggtccgg ttccggcact
    33241 ggcacctaaa cgtggcggtc gctggcgctg gcagatattg ttgcagcacc cttcccgcgt
    33301 gcgcttgcaa cacatcatta gtggtacgct ggcgctcatc aatacaatac cggattcccg
    33361 caaggtgaaa tgggtactgg atgttgatcc gattgagggt taaaccgctt acgatgcgag
    33421 gcggatcgaa aaattcaata ttcatcacac ttttcatgaa aattctgtaa ccgttttcac
    33481 gcgctatctg ctaaaaatgt tgccgatgtg aagtaaacat ggatgtagta cgcctgacgt
    33541 accaggcgag gagtgagtgt gaaagcgaag aagcaggaaa ctgccgcgac catgaaagac
    33601 gttgccctca aggcaaaagt ctctacagcg actgtctccc gagcattaat gaatcccgat
    33661 aaagtctccc aggccacccg taatcgggtt gaaaaagcgg cccgggaagt gggttattta
    33721 ccgcagccta tggggcgcaa cgtcaagcgt aatgaatccc gcaccattct ggtgattgtc
    33781 ccggatatct gcgatccctt ctttagcgaa attattcgcg gtatcgaagt tacggcggca
    33841 aatcacggat atctggtgct gattggcgac tgtgcgcatc aaaatcagca ggaaaaaacc
    33901 tttatcgatt tgatcatcac caagcaaatt gatggcatgt tgctgctggg ttcaaggctg
    33961 ccgtttgatg ccagcattga ggaacagcgt aatctgccgc cgatggtgat ggcgaacgaa
    34021 tttgcaccgg aactggagct gcctaccgtt catatcgaca atctgaccgc cgcatttgat
    34081 gcagtaaatt atttatatga gcaagggcat aaacggattg gctgtatagc cggtcccgaa
    34141 gagatgccac tgtgtcacta ccgcctgcaa ggctatgttc aggcgctgcg tcgctgcgga
    34201 attatggttg atccacaata catcgcccgt ggcgacttca ccttcgaagc cggaagcaaa
    34261 gcaatgcaac agctgcttga tcttccacaa ccgcctactg cagtcttctg ccatagcgat
    34321 gtgatggcgc tcggcgcact ttctcaggca aaacgccagg ggctgaaagt cccggaagac
    34381 ctttccataa tcggttttga taacatcgac ctgacgcaat tttgtgatcc gccgctgaca
    34441 accatcgcgc agccgcgtta cgaaatcggt cgggaagcta tgctgttatt gcttgatcaa
    34501 atgcaggggc aacacgttgg cagtggctct cgtttaatgg actgcgaact tatcatccgg
    34561 ggatcaacac gcgcgttacc ttaaagtaaa cctttaagac tgccgtgact ggtcaaaggc
    34621 ccgccgctta agtaacatgg cgggctgacg aacgaataaa tacagcgaaa cgatagtggc
    34681 acaacgagat tatgtacgcc gcagccaacc ggcaccttcg cggcgaaaaa agagcacctc
    34741 acggaaaaag caacgaaatc tgcctgcggt ttctcccgct atggtcgcta ttgctgccgc
    34801 cgttcttgtg acctttatcg gtggtctgta cttcattacg catcacaaga aagaagagtc
    34861 cgagacgctg caaagccaga aagtgaccgg aaacggacta ccaccaaaac cagaagaacg
    34921 ctggcgctac attaaagagc tggaaagtcg ccagccgggt gtgcgtgcgc ccacagaacc
    34981 ttctgccggt ggtgaagtga aaacgccgga gcaactgaca ccagaacaac gtcagcttct
    35041 tgaacaaatg caggctgata tgcgccagca gccaacgcag ctggttgagg ttccgtggaa
    35101 tgaacagacg ccggaacagc gtcagcaaac gctacaacgc cagcgtcagg cgcagcaatt
    35161 agcggagcag caacgcctgg ctcagcagtc gcgtacgact gaacaaagct ggcagcagca
    35221 gacgcgtacg tcgcaagccg cgccggtaca ggcgcaaccg cgccagtcta aaccagcttc
    35281 tactcagcag ccgtaccagg atctgctgca aacccctgcg cacacgaccg cgcagtcgaa
    35341 gccgcagcaa gctgcgccgg ttgctcgtgc cgctgacgcg ccaaaaccga cggcggagaa
    35401 aaaagacgaa cgccgctgga tggtgcagtg cggttcgttc agaggcgcgg aacaggcaga
    35461 gacggtacgt gctcagctgg cgttcgaagg atttgactcg aaaatcacca ccaacaatgg
    35521 ctggaatcgt gtggtcattg gcccggtgaa aggcaaagag aacgcagaca gcaccctcaa
    35581 tcggttgaag atggcgggtc atacaaactg cattcggctc gccgccgggg gttgaaaccc
    35641 tcaaaatccc ccccatctat aattgcatta tgccccgtac ttttgtacgg ggtttgtact
    35701 ctgtattcgt aaccaagggg tcagctcgtg acaactatag taagcgtacg ccgtaacggc
    35761 catgtggtca tcgctggtga tggtcaggcc acgttgggca ataccgtaat gaaaggcaac
    35821 gtgaaaaagg tccgccgtct gtacaacgac aaagtcatcg cgggctttgc gggcggtact
    35881 gcggatgctt ttacgctgtt cgaactgttt gaacgtaaac tggaaatgca tcagggccat
    35941 ctggttaaag ccgccgttga gctggcaaaa gactggcgta ccgatcgcat gctgcgcaaa
    36001 cttgaagcac tgctggcagt cgctgatgaa actgcatcgc ttatcatcac cggtaacggt
    36061 gacgtggtgc agccagaaaa cgatcttatt gctatcggct ccggcggccc ttacgcccag
    36121 gctgcggcgc gcgcgctgtt agaaaacact gaacttagcg cccgtgaaat tgctgaaaag
    36181 gcgttggata ttgcaggcga catttgcatc tataccaacc atttccacac catcgaagaa
    36241 ttaagctaca aagcgtaagg atctcccatg tctgaaatga ccccacgcga aatcgtcagc
    36301 gaactggata agcacatcat cggccaggac aacgccaagc gttctgtggc gattgctctg
    36361 cgtaaccgct ggcgtcgcat gcagctcaac gaagagctgc gccatgaagt gaccccgaaa
    36421 aatatcctga tgatcggccc gaccggtgtc ggtaaaactg aaatcgcccg tcgtctggct
    36481 aagctggcga atgcgccgtt catcaaagtt gaagcgacca aatttaccga agtgggctac
    36541 gtcggtaagg aagttgattc tattattcgc gatctgaccg atgccgccgt gaaaatggta
    36601 cgcgtccagg ctatcgagaa aaaccgttat cgcgctgaag aactggcaga agaacgtatt
    36661 ctcgacgtgc tgatcccacc tgctaaaaac aactggggac agactgaaca gcagcaggaa
    36721 ccttccgctg ctcgtcaggc attccgcaaa aaactgcgtg aaggccagct tgatgacaaa
    36781 gaaatcgaga tcgatcttgc cgcagcaccg atgggcgttg aaattatggc tcctccgggc
    36841 atggaagaga tgaccagcca gctgcagtcc atgttccaga acctgggcgg ccagaagcaa
    36901 aaagcgcgta agctgaaaat caaagacgcc atgaagctgc tgattgaaga agaagcggcg
    36961 aaactggtga acccggaaga gctgaagcaa gacgctatcg acgctgttga gcagcacggg
    37021 atcgtgttta tcgacgaaat cgacaaaatc tgtaagcgcg gcgagtcttc cggtccggat
    37081 gtttctcgtg aaggcgttca gcgtgacctg ctgccgctgg tagaaggttg caccgtttcc
    37141 accaaacacg ggatggtcaa aactgaccac attctgttta tcgcttctgg cgcgttccag
    37201 attgcgaaac cgtctgacct gatcccggaa ctgcaaggtc ggctgccaat ccgcgttgaa
    37261 ctgcaggcac tgaccaccag cgacttcgag cgtattctga ccgagccgaa tgcctctatc
    37321 accgtgcagt acaaagcact gatggcgact gaaggcgtaa atatcgagtt taccgactcc
    37381 ggtattaaac gcatcgcgga agcggcatgg caggtgaacg aatctaccga aaacatcggt
    37441 gctcgtcgtt tacacactgt tctggagcgt ttaatggaag agatttccta cgacgccagc
    37501 gatttaagcg gtcaaaatat cactattgac gcagattatg tgagcaaaca tctggatgcg
    37561 ttggtggcag atgaagatct gagccgtttt atcctataat cgcgttcaat cattttcatc
    37621 attgtttgat ggggctgaaa ggccccattt ttattggcgc gtattatgac tgaacaacaa
    37681 attagccgaa ctcaggcgtg gctggaaagt ttacgaccta aaaccctccc cctcgccttt
    37741 gctgcaatta tcgtcgggac ggcgctggca tggtggcaag gtcacttcga tccgctggtc
    37801 gccctgctgg cactaattac cgccgggcta ttacagatcc tttctaacct cgccaatgat
    37861 tacggcgatg cggtaaaagg cagcgataaa cctgaccgca ttgggccgct acgcggcatg
    37921 caaaaagggg tcattaccca gcaaaagatg aaacgggcgc tcattattac cgtcgtgctc
    37981 atctgtctct ccgggctggc actggttgca gtggcatgcc atacgctggc cgattttgtc
    38041 ggtttcctga ttcttggcgg gttgtcgatc attgccgcta tcacctacac cgtgggcaat
    38101 cgtccttatg gttatatcgg tctgggtgat atttccgtac tggttttctt tggctggttg
    38161 agtgtcatgg ggagctggta tttacaggct catacattga ttccggcact gatccttccg
    38221 gcgaccgcat gcggcctgct ggcaacggca gtactgaata ttaataacct gcgtgatatc
    38281 aatagcgacc gcgaaaatgg caaaaacacg ctggtggtgc gcttaggtga agtgaacgcg
    38341 cgtcgttatc atgcctgcct gctgatgggc tcgctggtgt gtctggcgct gtttaatctc
    38401 ttttcgctgc atagcctgtg gggctggctg tttctgctgg cggcaccatt actggtgaag
    38461 caagcccgtt atgtgatgcg ggaaatggac ccggtggcga tgcgaccaat gctggaacgt
    38521 actgtcaagg gagcgttact gactaacctg ctgtttgttt tagggatatt cctaagccag
    38581 tgggcagcat aactgacaaa tatcaattaa caattgatga ttttgccaac agcccacata
    38641 gcgcgatata ctgaaaattc tcgcagcaac tgaatgttaa gcctatgaaa tacgatactt
    38701 ccgagctttg tgacatctat caagaagatg ttaacgtcgt ggaaccgctg ttctccaact
    38761 ttggcggacg ggcgtcgttt ggcggacaaa taatcacggt aaaatgtttc gaggacaacg
    38821 ggttgctgta cgatctgctc gaacagaatg gccgtggtcg tgttcttgtc gtcgatggcg
    38881 gtggttctgt tcgtcgcgca ctggtcgatg ctgaactggc gcgtctggca gtacaaaatg
    38941 aatgggaagg tctggtcatt tacggcgcgg tgcgtcaggt agatgacctg gaagagttgg
    39001 atatcggcat ccaggcgatg gcggcaattc cggttggtgc cgctggcgaa ggcattggcg
    39061 aaagcgatgt ccgcgtcaat tttggcggtg tcaccttctt ctccggcgac catctttatg
    39121 ccgacaatac cgggattatt ctttcagaag atccgctaga tattgaatga taagaaaggc
    39181 accgcaaggt gccttttttc tgcgttacct gttggcctac acagtaaaga aattacgcga
    39241 aagatgaagc gtaatcagac ctcttccatg cgacccagca gggcctgcag acgttcctgc
    39301 cagccgttct gctgttcttt cagatggttg ttctcacgct ccagctcttc gcgctgatgc
    39361 tgggcatttt gaacttcctg cgacagtgag ttgttttttt ctttcagctc ttcgatttcc
    39421 atctgcaaca gagtgatggt atcaatcgcc tgctgtactt ttgcttccag tttatcaaac
    39481 acttctaatg acattgtcat acctctcctg aattgcaagg cgttgatgga taaaaaatcc
    39541 tcgtcccgat taccggtgac gccttaataa atacgagcgc actttagtta gctccgattg
    39601 tatgaagccg cgccatcgct gtccagcggc acgccttgca gattacggtt tgccacactt
    39661 ttcatccttc tcctagtgac ataatccaca tcaatcgaaa atgttaataa atttgttgcg
    39721 cgaatgatct aacaaacatg catcatgtac aatcagatgg aataaatggc gcgataacgc
    39781 tcattttatg acgaagcaca cacattttaa gttcgatatt tctcgttttt gctcgttaac
    39841 gataagttaa cagcatgcct acaagcatcg tggaggtccg tgactttcac gcatacaaca
    39901 aacattaact cttcaggatc cgattatgag tcaaacatca accttgaaag gccagtgcat
    39961 tgctgaattc ctcggtaccg ggttgttgat ttttttcggt gtgggttgcg ttgcagcact
    40021 aaaagtcgct ggtgcgtctt ttggtcagtg ggaaatcagt gtcatttggg gactgggggt
    40081 ggcaatggcc atctacctga ccgcaggggt ttccggcgcg catcttaatc ccgctgttac
    40141 cattgcattg tggctgtttg cctgtttcga caagcgcaaa gttattcctt ttatcgtttc
    40201 acaagttgcc ggcgctttct gcgctgcggc tttagtttac gggctttact acaatttatt
    40261 tttcgacttc gagcagactc atcacattgt tcgcggcagc gttgaaagtg ttgatctggc
    40321 tggcactttc tctacttacc ctaatcctca tatcaatttt gtgcaggctt tcgcagttga
    40381 gatggtgatt accgctattc tgatggggct tatcctggcg ttaacggacg atggcaacgg
    40441 tgtaccacgc ggccctttgg ctcccttgct gattggtcta ctgattgcgg tcattggcgc
    40501 atctatgggc ccattgacag gttttgccat gaacccagcg cgtgacttcg gtccgaaagt
    40561 ctttgcctag ctggcgggct ggggcaatgt cgcctttacc ggcggcagag acattcctta
    40621 cttcctggtg ccgcttttcg gccctatcgt tggcgcgatt gtaggtgcat ttgcctaccg
    40681 caaactgatt ggtcgccatt tgccttgcga tatctgtgtt gtggaagaaa aggaaaccac
    40741 aactccttca gaacaaaaag cttcgctgta atatgactac gggacaatta aacatgactg
    40801 aaaaaaatat atcgttgcgc tcgaccaggg caccaccagc tcccgcgcgg tcgtaatgga
    40861 tcacgatgcc aatatcatta gcgtgtcgca gcgcgaattt gagcaaatct acccaaaacc
    40921 aggttgggta gaacacgacc caatggaaat ctgggccacc caaagctcca cgctggtaga
    40981 agtgctggcg aaagccgata tcagttccga tcaaattgca gctatcggta ttacgaacca
    41041 gcgtgaaacc actattgtct gggaaaaaga aaccggcaag cctatctata acgccattgt
    41101 ctggcagtgc cgtcgtaccg cagaaatctg cgagcattta aaacgtgacg gtttagaaga
    41161 ttatatcagc agcaataccg gtctggtgat tgacccgtac ttttctggca ccaaagtgaa
    41221 gtggatcctc gaccatgtgg aaggctctcg cgagcgtgca cgtcgtggtg aattgctgtt
    41281 tggtacggtt gatacgtggc ttatctggaa aatgactcag ggccgtgtcc atgtgaccga
    41341 ttacaccaac gcctctcgta ccatgttgtt caacatccat accctggact gggacgacaa
    41401 aatgctggaa gtgctggata ttccgcgcga gatgctgcca gaagtgcgtc gttcttccga
    41461 agtatacggt cagactaaca ttggcggcaa aggcggcacg cgtattccaa tctccgggat
    41521 cgccggtgac cagcaggccg cgctgtttgg tcagttgtgc gtgaaagaag ggatggcgaa
    41581 gaacacctat ggcactggct gctttatgct gatgaacact ggcgagaaag cggtgaaatc
    41641 agaaaatggc ctgctgacca ccatcgcctg cggcccgact ggcgaagtga actatgcgtt
    41701 ggaaggtgcg gtgtttatgg caggcacatc cattcagtgg ctgcgcgatg agatgaagtt
    41761 gattaacgac gcctacgatt ccgaatattt cgccaccaaa gtgcaaaaca ccaatggtgt
    41821 gtatgtggtt ccggcattta ccgggctgag tgcgccgtac tgggacccgt atgcgcgcgg
    41881 ggcgattttc ggtctgactc gtggggtgaa cgctaaccac attatacgcg cgacgctgga
    41941 gtctattgct tatcagacgc gtgacgtgct ggaagcgatg caggccgact ctggtatccg
    42001 tctgcacgcc ctgcgcgtgg atggtggcgc agtagcaaac aatttcctga tgcagttcca
    42061 gtccgatatt ctcggcaccc gcgttgagcg cccggaagtg cgcgaagtca ccgcattggg
    42121 tgcggcctat ctcgcaggcc tggcggttgg cttctggcag aacctcgacg agctgcaaga
    42181 gaaagcggtg attgagcgcg agttccgtcc aggcatcgaa accactgagc gtaattaccg
    42241 ttacgcaggc tggaaaaaag cggtcaaacg cgcgatggcg tgggaagaac acgacgagta
    42301 atgtcaggca cctgaatcga ttgcctgatg ctgatcgcct gatgcgacgc ttacgcgtct
    42361 tatcaggcct acgagccaat gccattttgt gtaggcggga taaggcgttc acgccgcatc
    42421 cggcatcacg cagactctga aaccgtgcgg gggcaacccc gcacacatca ataatcccac
    42481 ccttcccctg tgctacactt cgcgccattc cttactgctt agagtttgct atgagacgag
    42541 aacttgccat cgaattttcc cgcgtcaccg aatctgcggc gctggctggc tacaaatggt
    42601 taggacgcgg cgataaaaac accgcggacg gcgcggcggt aaacgccatg cgtattatgc
    42661 tcaaccaggt caacattgac ggcaccatcg tcattggtga aggtgaaatc gacgaagcac
    42721 cgatgctcta cattggtgaa aaagtcggta ctggtcgcgg cgacgcggta gatattgctg
    42781 ttgatccgat tgaaggcacg cgcatgacgg cgatgggcca ggctaacgcg ctggcggtgc
    42841 tggcagtagg cgataaaggc tgcttcctca atgcgccgga tatgtatatg gagaagctga
    42901 ttgtcgggcc gggagccaaa ggcaccattg atctgaacct gccgctggcg gataacctgc
    42961 gcaatgtagc ggcggcgctc ggcaaaccgt tgagcgaact gacggtaacg attctggcta
    43021 aaccacgcca cgatgccgtt atcgctgaaa tgcagcaact cggcgtacgc gtatttgcta
    43081 ttccggacgg cgacgttgcg gcctcaattc tcacctgtat gccagacagc gaagttgacg
    43141 tgctgtacgg tattggtggc gcgccggaag gcgtggtttc tgcggcggtg atccgcgcat
    43201 tagatggcga catgaacggt cgtctgctgg cgcgtcatga cgtcaaaggc gacaacgaag
    43261 agaatcgtcg cattggcgag caggagctgg cacgctgcaa agcgatgggc atcgaagccg
    43321 gtaaagtatt gcgtctgggc gatatggcgc gcagcgataa cgtcatcttc tctgccaccg
    43381 gtattaccaa aggcgatctg ctggaaggta ttagccgcaa aggcaatatc gcgactaccg
    43441 aaacgctgct gatccgcggc aagtcacgca ccattcgccg cattcagtcc atccactatc
    43501 tggatcgcaa agacccggaa atgcaggtgc acattctctg agtgatttga tcgattgagc
    43561 cttccagtcc ttcgggactg gaattttttt gttcggagaa cgaagataag gcaagtcaat
    43621 caaaacagga gaaaaacatg gctgattggg taacaggcaa agtcactaaa gtgcagaact
    43681 ggaccgacgc cctgtttagt ctcaccgttc acgcccccgt gcttccgttt accgccgggc
    43741 aatttaccaa gcttggcctt gaaatcgacg gcgaacgcgt ccagcgcgcc tactcctatg
    43801 ttaactcgcc cgataatccc gatctggagt tttacctggt caccgtcccc gatggcaaat
    43861 taagcccacg attggcggca ctgaaaccgg gcgatgaagt gcaggtggtt agcgaagcag
    43921 ctggcttctt tgttctggat gaagtaccgg actgcgaaac gctatggatg ctggcaaccg
    43981 gtacagcgat tggcccttat ttatcgattc tgcaactagg caaagattta gatcgcttca
    44041 aaaatctggt cctggttcac gccgcacgtt atgccgccga cttaagctat ttgccactga
    44101 tgcaagaact ggaaaaacgc tacgaaggca aactgcgcat tcagacggtg gtcagtcggg
    44161 aaacggcagc ggggtcgctc accggacgga taccggcatt aattgaaagt ggggaactgg
    44221 aaagcgcgat tggcctgccg atgaataaag aaaccagcca tgtgatgctg tgcggcaatc
    44281 cacagatggt gcgcgataca caacagttgc tgaaagagac ccggcagatg acgaaacatt
    44341 tacgtcgccg accgggccat atgacagcgg agcattactg gtaagcggtt acttatcgat
    44401 aaacggcacg atgagcaaat ccgcactcat cttattgatc atcccgcgat atgccggcat
    44461 caaacggttg ataaatgagt gatgatgacc acagacaagg aggtcgcact gctctttttg
    44521 cataatttcc agcagtgttt ccggcatttc tccgcgttca atacgcagtt ttgtcttcgg
    44581 ccattgaata tttttcgtca gtttatacag cttgttatcc gacttattct tcaacaattg
    44641 aagaatatct tctgttgcag ggaagtagat acccggatac aactcgctta agccatcatc
    44701 aatatgaatt aacgtcaggt gagcgtcatt atgtctggcg agctccaggg ctttattcac
    44761 cagtaaggca tcttcttcat tcccggaaat tgccacgcca atgtgtttat aagccatgtt
    44821 taactccttc taaagcaact ccatcaagct aacgaacgca gtgataactc aaaaataatc
    44881 atctcggcct ggcatgagaa agtgaaggcc gcatcaagct caacttgccc ctccagatcc
    44941 ttaatcttat aggccacgct ggccccttca tctgcagaga atgcagcggc ggcaagctct
    45001 ttcagtttgg tcataaaaga ctctgcttct tcgcgggtgg caaaaaagcg cgaaaactta
    45061 ctggtacagt tgtcgttatc aatcaccgta ccgatatcaa tcgcacatcc tttagtactg
    45121 catttatcta cgacatcttt catagggcac ctctgtatat tcgcctctct gtcgattacg
    45181 acttaagaat aatgcgccgt taagggaaga acaaacaagt aatgatctta tggattacca
    45241 ttttttttga tattgcttaa ggtgaagagt tcagaaggat tttccgggta ttgtcatttg
    45301 catcgtggaa ataacaatcc cggaaaaaaa tagcagtaag agatatatta aaggtcaatt
    45361 acttgattta ttgtcggctt tatacttcac atcctgagta tctttaccat atttattttc
    45421 gccttgtgtg ccaacaaacg caccaagatc gataagcatc atcaccagaa tcaacgtcgg
    45481 gacaaaacgc cccaccgccc attgccagac acccggtaaa atcgctcagt tacccgccag
    45541 cagcatccac gccacaatca tcagaaatgc ccatgcgccg gaacgcccgc gatcatgcaa
    45601 gcgcttaaca gttactgccg ctgttggcca gagcaagcac acaaggcaaa acgccgcggt
    45661 ctgaatatcg agtagattct tacccgccag tgagaaaagc accagcatgc ctgcgaacca
    45721 caggcctatc caaatccaga aatcacggcg tccaatacgc cctttaaatg agaataacca
    45781 ttgctgtatg gtcatgtaag ttccttgatg gttgtctttt ccaggattct acccttttga
    45841 caagggcgac agatatcgtt ttaatcggag ccagtcataa caaaaggtac tgtcaatgaa
    45901 gccagggtgt acgctgtttt ttctcttatg ttctgcatta accgttacaa caacggcgca
    45961 tgcacaaaca ccagatacgg caacgaccgc gccttatctg ctggctggag cccctacttt
    46021 cgatctctcc atcagccagt ttcgagaaga ctttaacagc cagaatccca gcctgccact
    46081 gaacgaattt cgtgccatcg acagcagtcc cgacaaagcc aatctcactc gtgctgcaag
    46141 taaaattaac gagaacttgt atgcttctac agcgctggag cgcggtacct taaaaatcaa
    46201 aagcattcaa atgacctggc tacccatcca ggggccagag caaaaagcag cgaaagcgaa
    46261 agctcaggaa tacatggcag cagtgatccg cacactcacc ccattaatga ccaaaacaca
    46321 aagccagaaa aaactgcagt cgctactaac ggcggggaaa aacaaacgtt attacaccga
    46381 gacagaaggt gcactgcgtt atgttgtcgc ggacaacggc gaaaaggggc tgaccttcgc
    46441 tgttgaaccg attaagctgg cgctatctga atcgcttgaa ggtttgaata aatgacaaaa
    46501 agcaaagcct ttgtgccgat gaatctctat actgtttcac agacctgctg ccctgcgggg
    46561 cggccatctt cctttattcg cttataagcg tggagaatta aaatgcgaca tcctttagtg
    46621 atgggtaact ggaaactgaa cggcagccgc cacatggttc acgagctggt ttctaacctg
    46681 cgtaaagagc tggcaggtgt tgctggctgt gcggttgcaa tcgcaccacc ggaaatgtac
    46741 atcgatatgg cgaagcgcga agctgaaggc agccacatca tgctgggtgc gcaaaacgtg
    46801 gacctgaacc tgtccggcgc attcaccggt gaaacttctg ccgctatgct gaaagacatc
    46861 ggcgctcagt acatcatcat cggccactct gaacgtcgta cttaccacaa agagtctgac
    46921 gaactgattg cgaaaaaatt cgcggtgctg aaagagcagg gtctgactcc ggttctgtgc
    46981 atcggtgaaa ccgaagctga aaacgaagcg ggaaaaactg aagaagtttg cgcacgtcag
    47041 atcgacgcgg tactgaaaac tcagggtgct gcggcattcg aaggtgcggt tatcgcttat
    47101 gaacctgtat gggcaatcgg tactggcaaa tctgcaactc cggctcaggc acaggctgtt
    47161 cacaaattca tccgtgacca catcgctaaa gttgacgcta acatcgctga acaagtgatc
    47221 attcagtacg gcggctctgt aaacgcgtct aacgctgcag aactgtttgc tcagccagac
    47281 atcgacggcg cgctggttgg cggtgcttct ctgaaagctg acgctttcgc agtgatcgtt
    47341 aaagctgcag aagcggctaa acaggcttaa gtctgacagg tgccggattt catatccggc
    47401 acttactttc cttaactcct cgccttaacg caaaatctca cactcatgat cctggatttc
    47461 ctcggctgaa gcacggttca gcgtcagtag atttcgttgt gtcgccagca acacaaacga
    47521 attatcactc tgccgtacca tcgccagccc gtagcttccc atatgttccc gcgcctcagg
    47581 cacttcttct gcaagcatca taaatgggct gcgttgtacc agttcgcttt ccgttacccg
    47641 acgcgccagg tattcatgcc cgcgcaaacc acctggcagt ggcaaccagc ggctgctgat
    47701 gttcgccaga tttttatcca gttgttcgcg cacatcagga cgaatacaag agatatgaat
    47761 atgaaaatgg ttttgcgtac gcccggtgcg ggagttgatc gccaaagaaa ccgcgcgatc
    47821 gggaaccggc tggccgtatt ttttgctcat aaaatcacgc gcctgccagg ccaaccaaaa
    47881 gaagttcggc gttgaaggat cggtcaacaa aggactttca gtaccgttaa tacgatacgt
    47941 tgacatcaac agatattgca gtggaccatt aagatctttt aaaaccacgt atccggcatt
    48001 gggtttgact tccgcacatg gcgaaggatt ttgattttgc tgctgattgg gcaaacattc
    48061 ctcaaggaca atcttacgta atgtatccga ctcttcaccg gttaatttcc agtaaccaat
    48121 accggcagcc acaacggcga taactatcat caccaaaaaa agaagacccg cttttttcat
    48181 ctttttttcc ctgtacctca aagagatgca agggtaacgc aaaatcgtga caaataaaaa
    48241 acccggtcgc gagaaaccac cgggtttgat aattatccta gggaaatcag cgtttgctga
    48301 tctgatcgaa cgtaccgccg ttagcaaaat gctctttttg cgctttcgtc cagccgccga
    48361 actcttcatc aatggtgaat aacttcagct ttggaaacgc attttcgtac tttttcgcta
    48421 cttcagcgtc gcgcggacgg tagtagtttt tcgcggcaat ttcctgacct tctggcgagt
    48481 agagatattt caggtaggct tccgccacct ctttggtgcc ttttttctcg accactttat
    48541 cgaccaccga caccgttggc tctgcgagga tagactcact cggcgtgacg atttcgaatt
    48601 tatctttccc cagttcattc gctgccagca gagcttcgtt ttcccaggcg atcagtacat
    48661 cgccaatccc gcgctcgaca aaggtgttag tggagccgcg cgcgccagaa tccagaactt
    48721 cgacgttttt atacagtgcc cgaacaaaat cctgtgcttt tgcctgatcg ttgttgttgt
    48781 gatgcagcgc gtagccccag gcagccaggt agttccagcg tgcgccacca gagcttttcg
    48841 gattaggcgt gatcaccgaa acacccggtt taatcagatc gttccagtca tggatctgct
    48901 tcggattgcc cttacgcacc aggaaaacaa tggtggaagt gtacggtgcg gagttatccg
    48961 gcagacgttt gatccactct ttatcaatcc gcccgcgttc cgcaatcgcg tccacgtcat
    49021 aggccagagc cagcgtgaca acatcagctt caataccgtt gattaccgac gtcgcttgtt
    49081 tacctgaacc accgtgtgac tgacgaatca ccacgttatc accagtttgc tgtttccagt
    49141 gggcgctgaa tgccttgttg tactgttcgt acaattcgcg cgttggatca tatgaaacgt
    49201 taagaagctg aatatccttt gccataacgc tggttgccgc cagcaaaaat gttaacccta
    49261 cgccccactt gttcatcgcc cgactctctt atgttgtgtt gtgatgagca aagcgtgcca
    49321 gaaggttaac caaacattaa agaataaaaa aagattggct ataacttgcg ggtatatgtt
    49381 gagggattaa aaaggcggaa gaaatccgcc tcatattgct gacaaagtgc gctttgtcca
    49441 tgccggatgc ggcgcgaacg ccttatccgg cctacaaaag tttgcaaatt caataaattg
    49501 cagaattcat gtaggcctga taagcgaagc gcatcaggca tttttgcttc tgtcatcggt
    49561 ttcaggctaa aggaatctgc ctttttccga aatcattaat acagtttttt cgcgcagtcc
    49621 agccagtcac ctttgaacgg acgcttcatg ttttcgatag cgtcgatgat gtcgtggtga
    49681 accagctgtt cgttctggat acctacacaa cgaccgccgt aacctgccag cagcagatcg
    49741 atagcgtaag cgcccatacg ggaagccaga atacggtcgt aaggcaccgg agaaccaccg
    49801 cgctggatgt ggcccagcac agttgcgcgg gtttcacgac cggtttcttt ctcgatgaaa
    49861 tgcgccagtt cgtcaacatc acacatatgt tcggtaatcg ccacgatcgc gtgtttttta
    49921 cctttcgcga tacccgcttt gatttcgttt accaggtctt cacggctgaa ttcaacttcc
    49981 ggaactacaa cgaattcaca gccaccggca atggccgcag ccagggtcag atcgccacaa
    50041 taacggccca tcacttccac cacggaaata cgctggtgag aagaagaggt gtcacgcaga
    50101 cggtcgatcg cttctacaac ggtgctcagc gcagtgaaga aaccgatagt gtagtcagtg
    50161 cctttgatgt cgttgtcgat agtgcccggc agaccgatgc acgggaagcc catttcggtc
    50221 agacgcattg cacccatgta ggaaccgtca ccgccgataa ccaccagcgc gtcgatacca
    50281 cgttttttca ggttttcgat agccacggcg cggatgttct cgtcgcggaa ttccgggaaa
    50341 cgcgcagaac cgaggaacgt accgccacgg ttgatcatgt cagacacgct gtaacggtct
    50401 agctgtacca tacggtcttc atacagaccc agatagccgt cataaatacc cattacttcc
    50461 agaccttctg tcagcgcaga acgaacaacc ccgcgaattg cggcgttcat gcctggcgca
    50521 tcaccgccgc ttgtcaacac acctattttc ttaatcatga ctacctctga actttggaat
    50581 gcaaaatgaa atctgttgcc ggaaatcttc ttgcacatcg aagtgatcca acgaatgtgc
    50641 aaatagtata acaatcactt cctgctgaat tgattcaggt caggccaaat ggcggtattt
    50701 tatacacaaa atgcgggtct ggctctcttt tatactgatt atgaaagcat agaccgttta
    50761 ccctccctgg gtacgacgga acaggggtcc tgatggataa tcacatccga tcccggaaaa
    50821 cgccgtaaaa tagcctgctc tacctgatcc gccaccatat gtgcctgaac caaaggcaga
    50881 gagtcttcca tttccaaatg aatctgaata aagcgggtcg gccctgactg ccgcgtgcga
    50941 agatcgtgag cgccgctaac acccggccag gaagtcacga tatcaataat ttcttgccgt
    51001 tcttcatcag gcaatgcgcg atccagtaat gactgtaccg cctcatatcc catgcgtaac
    51061 gcgctatata aaatatagat gccgattccc aatgcaaaca gagcatcggc gcgatgccag
    51121 ccgtaccaag acaaccccag cgccagcaga attgcgccgt tcatcataac atcagactgg
    51181 taatgtagca tatcagcccg caccgcctgg ctttgcgtcc ggcgcaccac ccaccgctga
    51241 aacgagacaa ggataatcgt acaaattagc gccacaattg tcacgataac cccgacgcct
    51301 ggatctgtca tcggtgttgg agataccaga tgttgaatac ccgtcaaaaa caggaatagt
    51361 gccgaaccgg agataaacat actttgcgcc agcgccgcga gggactcagc tttaccgtga
    51421 ccaaacgagt gattatcgtc ggcaggttgc agggaatatc gcaccaccag taaattcgtc
    51481 aacgacgcgc cgatatccac cagcgaatcc accagcgcgg cgagaatact caccgacccg
    51541 gtataccacc atgcaaaaat tttaatcagc aatagcaacg aagccatcgc cgtcgcagca
    51601 atcgccgccc gactgaccag ccgtccataa gattgattca taaatactcc cgctatcaac
    51661 tgacgctagt ataacggaag caaatcatct gcaatgcatt aagcagcagg caaattgagg
    51721 ataaaaaaaa ccccacatca tgtgggggaa gacagggatg gtgtctatgg caaggaaaac
    51781 agggtttact actgggaacg tgagttgcta ctactcaata gcttcaacga tgaacttttt
    51841 tgccattgcg tcacgtcacg caactgctcc attcgttgtt gatgtttctc gtttaaaacc
    51901 gcttgctgct ccggcgttaa caggcgatac atttggttgc ggacttttgc catctcaacc
    51961 tgacgagcaa tttgctcatt cgccattttt tctgcctgtg cgcgcacagc gttttcatca
    52021 aaattttctg cggtgacaag gcgatgcatt gtctccagtt cgctaacatt aacaggaggc
    52081 tgttcgtgcc gggcctgttg cataagatct cgcatctgct gacgctgatg ttcggttaaa
    52141 cttatgccgt cgaacatatg gctctgcgta ctgcgctgcg taagttcttc acccggatgc
    52201 cagttatcgc ctgaaccgac ttcagcagcg tggcttaatg aactgactgc cagcgttgag
    52261 gccatgacgg cagcggtaac tatgcgcatc atttgctccc aaaatctttc tgtcgcgatt
    52321 caacgataga gagtttacga ttcaggctgc aaacatgcgt cagggggtgt aaaacaacgt
    52381 aaagtcatgg attagcgacg tctgatgacg taatttctgc ctcggaggta tttaaacaat
    52441 gaataaaatc ctgttagttg atgatgaccg agagctgact tccctattaa aggagctgct
    52501 cgagatggaa ggcttcaacg tgattgttgc ccacgatggg gaacaggcgc ttgatcttct
    52561 ggacgacagc attgatttac ttttgcttga cgtaatgatg ccgaagaaaa atggtatcga
    52621 cacattaaaa gcacttcgcc agacacacca gacgcctgtc attatgttga cggcgcgcgg
    52681 cagtgaactt gatcgcgttc tcggccttga gctgggcgca gatgactatc tcccgaaacc
    52741 gtttaatgat cgtgagctgg tggcacgtat tcgcgcgatc ctgcgccgtt cgcactggag
    52801 cgagcaacag caaaacaacg acaacggttc accgacactg gaagttgatg ccttagtgct
    52861 gaatccaggc cgtcaggaag ccagcttcga cgggcaaacg ctggagttaa ccggtactga
    52921 gtttaccctg ctctatttgc tggcacagca tctgggtcag gtggtttccc gtgaacattt
    52981 aagccaggaa gtgttgggca aacgcctgac gcctttcgac cgcgctattg atatgcacat
    53041 ttccaacctg cgtcgtaaac tgccggatcg taaagatggt cacccgtggt ttaaaacctt
    53101 gcgtggtcgc ggctatctga tggtttctgc ttcatgatag gcagcttaac cgcgcgcatc
    53161 ttcgccatct tctggctgac gctggcgctg gtgttgatgt tggttttgat gttacccaag
    53221 ctcgattcac gccagatgac caagcttctg gatagcgaac agcgtcaggg gctgatgatt
    53281 gagcagcatg tcgaagcgga gctggcgaac gatccgccca acgatttaat gtggtggcgg
    53341 cgtctgttcc gggcgattga taagtgggca ccgccaggac agcgtttgtt attggtgacc
    53401 accgaaggcc gcgtgatcgg cgctgaacgc agcgaaatgc agatcattcg taactttatt
    53461 ggtcaggccg ataacgccga tcatccgcag aagaaaaagt atggccgcgt ggaactggtc
    53521 ggtccgttct ccgtgcgtga tggcgaagat aattaccaac tttatctgat tcgtccggcc
    53581 agcagttctc aatccgattt cattaactta ctgtttgacc gcccgctatt actgctgatt
    53641 gtcaccatgt tggtcagtac gccgctgctg ttgtggttgg cctggagtct ggcaaaaccg
    53701 gcgcgtaagc tgaaaaacgc tgccgatgaa gttgcccagg gaaacttacg ccagcacccg
    53761 gaactggaag cggggccaca ggaattcctt gccgcaggtg ccagttttaa ccagatggtc
    53821 accgcgctgg agcgcatgat gacctctcag cagcgtctgc tttctgatat ctctcacgag
    53881 ctgcgcaccc cgctgacgcg tctgcaactg ggtacggcgt tactgcgccg tcgtagcggt
    53941 gaaagcaagg aactcgagcg tattgaaacc gaagcgcaac gtctggacag catgatcaac
    54001 gatctgttgg tgatgtcacg taatcagcaa aaaaacgcgc tggttagcga aaccatcaaa
    54061 gccaaccagt tgtggagtga agtgctggat aacgcggcgt tcgaagccga gcaaatgggc
    54121 aagtcgttga cagttaactt cccgcctggg ccgtggccgc tgtacggcaa tccgaacgcc
    54181 ctggaaagtg cgctggaaaa cattgttcgt aatgctctgc gttattccca tacgaagatt
    54241 gaagtgggct ttgcggtaga taaagacggt atcaccatta cggtggacga cgatggtcct
    54301 ggcgttagcc cggaagatcg cgaacagatt ttccgtccgt tctatcggac cgatgaagca
    54361 cgcgatcgtg aatctggcgg tacaggattg gggctggcga ttgttgaaac cgccattcag
    54421 cagcatcgtg gctgggtgaa ggcagaagac agcccgctgg gcggtttacg gctggtgatt
    54481 tggttgccgc tgtataagcg gagttaaact ccgcatttgt aggcaggata aggcgtttac
    54541 gccgcatccg gcatttgagc aggatgcctg atgcgacgct gatagcgtct tatcaggcct
    54601 acactccggg ggttattttc cccacaaccg gcgggaaaaa tcttcgatct tgccgctcag
    54661 tcggcgcttt tgcatcgtcc gcgtccagct tttcgataac ccggcggcgg agagtaaacg
    54721 gtgatactga tcgtcatcaa acggcatatg ccatgcgatg gcggcagcct cctgcacggt
    54781 cacatcgctg acacgggaaa ccaactccag cggcgcgtcc gcagacactt tccctggcgc
    54841 tatcacgcta tacaaccacc ccaccttacc tgtgttttgc atcaactgcg ctatatcgct
    54901 gatatcaaaa tggtaattga gtttatagca aggcgagcgc ggctggctga cctgaattaa
    54961 tgcctctccc cagcggaaaa tatcgcccat atgaacatta ctttccgtga gaccgtcggt
    55021 tgagagattt tcgccaaacg caggcgcaac aaacaactcc gcctgttccg gaaattcccg
    55081 cacccagtag agatagtgct cacgaggata atgacacagc gctctgtctg gcccgccgtg
    55141 aacctttttc tccgcctgct cgtcaccttc cagccccagc tccgtcagca tcaactcacc
    55201 atcaacctgg attttggcaa ttgcgctggg tttgccttcg ggataagcct gaatcttgcc
    55261 tgtgtataca tcaaccggat atcgcatctg ttccctctcc acgagaaatt tccccattag
    55321 accatattta ttttatgtca gccgtgctgt tttcgcggta agtatccagg caggaactca
    55381 atagcaaacc ggacgaacgt tagttcatcc ggctgtgggg attaaggaag caggtttatt
    55441 tcaccgtacc taaaatttcg attttcgctg ctctggcttt gactttacgt gaccagatag
    55501 aggtaagaat tggcaccaga atcgaagtca caatgaccgc cgtcgctacc agcgaagttg
    55561 ctgccggagc catcggttta aacgcaggca ccatttctgc aatcagcaca ggtgtcgcta
    55621 ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt gccatcgcca ccgccaatca
    55681 atttatcggc gataatcaac ggaataccgg tcacgataat tattgccaca cccaacagga
    55741 tccccagcaa accagtctgg gcaatcacag tcaaatcaat ggtattgccc agcgcgaagg
    55801 caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa ttctcgtaat tcagggtcaa
    55861 gattccccag ggcaaagccc accaggaacg gtaatactgc gccgacgaaa acatgcggtt
    55921 caaacgaggc aatcccggca gtgcccagaa taatcatcgt catgagcggc ccggactcca
    55981 acgacatcaa cacaaatgcc ccagcttctt ctttcgtacc gtactgctgc atgatggaag
    56041 cgtaaagtcc gccgttggtc atatccatcg ccgccaccag cgccagcgtt gaaagtccgg
    56101 caaagaatcc aacttcaaca ccatgttccg gaataatgcg cgaggcaatt gccgcaacca
    56161 cccacgcgac ggcaatttta gttaccacca gcgtaccgga tttacgcagt accgttcccg
    56221 tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca caccgccaga atgggcaccg
    56281 taccggtaat catcccgttg gtgaatgatc caaaatattt ccccgccccc ggcgagaagg
    56341 tgtggcacag tgcgccaagg aatagcggga cgagcatcat cccccccggg attttctcaa
    56401 tcgagcgttt tatctgcatt tccatcacct gtcggcatat ttttaagggc tgatgcgtaa
    56461 ttcctcaatg gagcatcagt gttgatggaa acgaagatag acattcattt aatcaataaa
    56521 agtgatcaaa accacatcaa ttgaaacgct gttttatttt tataggatca ttgtttttat
    56581 tttggaattg atcacaaaaa aaccaccgcc gttggcgatg gttcattttt taagctgata
    56641 tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt attttttcgc cgcaaaacgt
    56701 gccgctgctt cgtcccagtt caccacgttc cagaactctt tgatgtagtc cggacggcgg
    56761 ttctggaatt tcaggtagta agcatgttcc cacacatcca ggcccagaat cgggaagccg
    56821 gaagcgccag aaatagcttc acccatcagc ggagaatcct ggttagcagt agaaaccacc
    56881 gccagtttat cgcctttcag caccagccat gcccagccgg aaccaaagcg ggaagctgcc
    56941 gctttttcaa attctgcttt gaagttatca acggagccga agtcacgttc gatagccgct
    57001 ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt tccagaacag gctgtggtta
    57061 gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt ctgctggcag ctggtccagt
    57121 ttggtgatca gctcttcaac cggcaggttg gcaaattctg gcaggctttc cagcgccgcg
    57181 ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt ggatttccat ggtctgctta
    57241 tcgaagtgcg gttccagggc atcgtaagca tacggcaggg atggcagggt atagctcata
    57301 ttcatctcca gtattgtcgg gcggccgatt gttaatgccg cgtaagcagt tggtttatta
    57361 tagttaatta aatgatattg aaaatgatta tcaatgccgc acttttcgta agggtatggt
    57421 tttgcaggaa aatgcccgag atgtgaagca aatcacccac ttaatgccgt gattgccagt
    57481 aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca gcacgatttt tacactcatc
    57541 tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag aagatgagta acgcgattac
    57601 gatggggata ttttggcatt tgatcggcgc ggccagtgca gcctgttttt acgctccgtt
    57661 caaaaaagta aaaaaatggt catgggaaac catgtggtca gtcggtggga ttgtttcgtg
    57721 gattattctg ccgtgggcca tcagcgccct gttactaccg gatttctggg cgtattacag
    57781 ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc ggcgctatgt gggggatcgg
    57841 taatatcaac tatggcctga ccatgcgtta cctcggcatg tcgatgggaa ttggcatcgc
    57901 cattggcatt acgttgattg tcggtacgct gatgacgcca attatcaacg gcaatttcga
    57961 tgtgctgatt aacaccgaag gcggacgcat gacgttactc ggcgttctgg tggcgctgat
    58021 tggcgtaggg attgtaactc gagccgggca gttgaaagag cgcaagatgg gcattaaagc
    58081 cgaagagttc aatctgaaaa aagggctggt gctggcggtg atgtgcggca ttttctctgc
    58141 cgggatgtcc tttgcgatga acgccgcaaa accgatgcat gaagccgctg ccgcacttgg
    58201 cgtcgatcca ctgtatgtcg ctctgccaag ctatgttgtc atcatgggcg gcggcgcgat
    58261 cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg aaggatttgt cgctaaaagc
    58321 cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg ttactctcgg cactgggcgg
    58381 gttgatgtgg tatctgcaat tctttttcta tgcctagggc cacgcccgca ttccggcgca
    58441 gtatgactac atcagttgga tgctgcatat gagtttctat gtattgtgcg gcggtatcgt
    58501 cgggctggtg ctgaaagagt ggaacaatac aggacgccgt ccggtaacgg tgttgagcct
    58561 cggttgtgtg gtgattattg tcgccgctaa catcgtcggc atcggcatgg cgaattaatc
    58621 tttctgcgaa ttgagatgcg ccactggctg ggcgtcatcc cggtttcccg ggtaaacacc
    58681 accgaaaaat agttactatc ttcaaagcca cattcggtcg aaatatcact gattaacagg
    58741 cggctatgct ggagaagata ttgcgcatga cacactctga cctgccgcag atattgattg
    58801 atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa cgcgctcact gcacgatgcc
    58861 tcatcacaaa atttatccag cgcaaaggga cttttcaggc tagccgccag ccgggtaatc
    58921 agcttatcca gcaacgtttc gctggatgtt ggcggcaacg aatcactggt gtaacgatgg
    58981 cgattcagca acatcaccaa ctgcccgaac agcaactcag ccatttcgtt agcaaacggc
    59041 acatgctgac tactttcatg ctcaagctga ccgataacct gccgcgcctg cgccatcccc
    59101 acgctaccta agcgccagtg tggttgccct gcgctggcgc taaatcccgg aatcgctccc
    59161 tgccagtcaa gattcagctt cagccgctcc gggcaataaa taatattctg caaaaccaga
    59221 tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa tataaaagag atcgccacgg
    59281 gtaatgcgat aaggacgatc gttgagtaca tgcagaccat taccgcgcca gacaatcacc
    59341 agctcacaaa aatcatgtgt atgttcggca aagacatctt gcggataacg gtcagccaca
    59401 gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga gaagttttaa ctgatgcgcc
    59461 accgtggcta cctcggccag agaacgaagt tgattattcg caatatggcg tacaaatacg
    59521 ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc tggcgaatat cacgcggtga
    59581 ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta ctgtcgctga atccacagcg
    59641 ataggcgatg tcagtgacgc tggcctcgct gtggcgtagc agatgtcagg ctttcatcag
    59701 tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt tgctgcttaa gctgccgatg
    59761 tagcgtacgc agtgaaagag aaaattgatc cgccacggca tcccaattca cctcatcggc
    59821 aaaatggtcc tccagccagg ccagaagcaa gttgagacgt gatgcgctgt tttccaggtt
    59881 ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc ataaacaaga tctcgcgact
    59941 ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc atctgtgcaa ccagctgtcg
    60001 cacctgctgc aatacgctgt ggttaacgcg ccagtgagac ggatactgcc catccagctc
    60061 ttgtggcagc aactgattca gcccggcgag aaactgaaat cgatccggcg agcgatacag
    60121 cacattggtc agacacagat tatcggtatg ttcatacaga tgccgatcat gatcgcgtac
    60181 gaaacagacc gtgccaccgg tgatggtata gggctgccca ttaaacacat gaatacccgt
    60241 gccatgttcg acaatcacaa tttcatgaaa atcatgatga tgttcaggaa aatccgcctg
    60301 cgggagccgg ggttctatcg ccacggacgc gttacaagac ggaaaaaaat ccacactatg
    60361 taatacggtc atactggcct cctgatgtcg tcaacacggc gaaatagtaa tcacgaggtc
    60421 aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa aacgtcgatt tttcaagata
    60481 cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac cacaattcag caaattgtga
    60541 acatcatcac gttcatcttt ccctggttgc caatggccca ttttcctgtc agtaacgaga
    60601 aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga aattcagcag gatcacatta
    60661 tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc cagtgggcgc gtgatgctgg
    60721 cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga aatccatcgt tttaacaatg
    60781 ggctgcatag ccaaaacggt tatgtcacct gggatgtgga tagcctggaa agtgccattc
    60841 gccttggatt aaacaaggtg tgcgaggaag ggattcgtat cgatagcatt gggattgata
    60901 cctggggcgt ggactttgtg ctgctcgacc aacagggtca gcgtgtgggc ctgcccgttg
    60961 cttatcgcga tagccgcacc aatggcctga tgccgcaggc acaacagcaa ctcggcaaac
    61021 gcgatattta tcagcgtagc ggcatccagt ttctaccctt caatacgctt tatcagttgc
    61081 gtgcgctgac ggagcaacaa cctgaactta ttccacacat tgctcacgct ctgctgatgc
    61141 cggattactt cagttatcgc ctgaccggca agatgaactg ggaatatacc aatgccacca
    61201 ccacacaact ggtcaatatc aatagcgacg actgggacga gtcgctactg gcgtggagcg
    61261 gggccaacaa agcctggttt ggtcgcccga cgcatccggg taatgtcatt gggcactgga
    61321 tttgcccgca gggtaatgag attccggtgg tcgccgttgc cagccatgat accgccagcg
    61381 cggttatcgc ctcgccgtta aacggttcac gcgccgctta tctctcttct ggcacctggt
    61441 cattgatggg cttcgaaagc cagacgccat ttaccaatga cacggcgctg gcagccaaca
    61501 tcaccaatga aggcggggcg gaaggtcgct atcgggtgct gaaaaatatt atgggcttat
    61561 ggctgcttca gcgagtgctt caggaacggc aaatcaacga tctcccggcg cttatcgccg
    61621 cgacacaggc acttccggcc tgccgcttca tcatcaatcc caatgacgat cgctttatta
    61681 atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg ggaaacggcg caaccgatcc
    61741 cgggaagtga tgctgaactg gcgcgctgta ttttcgacag tctggcgctg ctgtatgccg
    61801 atgtgttgca tgagctggcg cagctgcgcg gtgaagattt ctcgcaactg catattgtcg
    61861 gcggcggctg ccagaacacg ctgctcaacc agctatgcgc cgatgcctgc ggtattcggg
    61921 tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat cggcatcgag ttaattacgc
    61981 tggatgaact caacaatgtg gatgatttcc gtcaggtcgt cagcaccacc gcgaatctga
    62041 ccacctttac ccctaatcct gacagtgaaa ttgcccacta tgtggcgcag attcactcta
    62101 cacgacagac aaaggagctt tgcgcatgac cactcaactg gaacaggcct gggagctggc
    62161 gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag gcgctgcgcc aacttgatcg
    62221 tttacccgtt tcaatgcact gctggcaggg cgatgatgtt tccggttttg aaaacccgga
    62281 aggttcgctg accgggggga ttcaggccac aggcaattat ccgggcaaag cgcgtaatgc
    62341 cagtgagcta cgtgccgatc tggaacaggc tatgcggctg attccggggc cgaaacggct
    62401 taatttacat gccatctatc tggaatcaga tacgccagtc tcgcgcgacc agatcaaacc
    62461 agagcacttc aaaaactggg ttgaatgggc gaaagccaat cagctcggtc tggattttaa
    62521 cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt acgctctcgc atcccgacga
    62581 cagcattcgc cagttctgga ttgatcactg caaagccagc cgtcgcgttt cggcctattt
    62641 tggcgagcaa ctcggcacac catcggtgat gaacatctgg atcccggatg gtatgaaaga
    62701 tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg gcagcactgg atgaggtgat
    62761 cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt gagagcaaat tgtttggcat
    62821 tggcgcagag agctacacgg ttggctccaa tgagttttac atggggtatg ccaccagccg
    62881 ccagactgcg ctgtgcctgg acgccgggca cttccacccg actgaagtga tttccgacaa
    62941 gatttccgcc gccatgctgt atgtgccgca gttgctgctg cacgtcagcc gtccggttcg
    63001 ctgggacagc gatcacgtag tgctgctgga tgatgaaacc caggcaattg ccagtgagat
    63061 tgtgcgtcac gatctgtttg accgggtgca tatcggcctt gacttcttcg atgcctctat
    63121 caaccgcatt gccgcgtggg tcattggtac acgcaatatg aaaaaagccc tgctgcgtgc
    63181 gttgctggaa cctaccgctg agctgcgcaa gctggaagcg gcgggcgatt acactgcgcg
    63241 tctggcactg ctggaagagc agaaatcgtt gccgtggcag gcggtctggg aaatgtattg
    63301 ccaacgtcac gatacgccag caggtagcga atggctggag agcgtgcggg cttatgagaa
    63361 agcgattttg agccagcgtg ggtaaacact gccggatgcg gcacgagcgc cttatccggc
    63421 ctacgggtcg aatgtccatg cgggctgtca ccctcacccc ggccctctcc ctgaaagggc
    63481 gagggagaaa aacatagcct cgtgcggcaa cggagctgaa caccgctccg gacagtcccc
    63541 tcgcccctac ggggagaggg ttagggtgag gggaacaagc cgcaccatgt ctaaacaagg
    63601 caacctaatt tacaggaaca cagaacatgc aaaacattac ccagtcctgg tttgtccagg
    63661 gaatgatcaa agccaccacc gatgcctggc tgaaaggctg ggacgagcgc aacggcggca
    63721 acctgacgct gcgcctggat gacgccgata tcgcgcttta tcacgacaac ttccacccac
    63781 aaccgcgcta tattccgctc agccaaccca tgcctttact ggcaaatacg ccgtttattg
    63841 tcaccggctc cggcaaattc ttccgtaacg tccagctcga tcctgcagct aacttaggcg
    63901 tcgtaaaagt cgacagcgat ggcgcgggct accatattct ctgggggtta accaacgaag
    63961 ccgttccaac ttctgaactt ccggctcact ttctctccca ctgcgagcgc attaaagcca
    64021 ccaacggtaa agatcgggtg atcatgcact gccacgccac caacctgatc gccctcacct
    64081 atgtactgga aaacgacacc gcggtcttta ctcgccaact gtgggaaggc agcaccgaat
    64141 gtctggcagt attcccggat ggcgttggca ttctgccgtg gatggtgccc ggcacggacg
    64201 aaatcggcca ggcgaccgca caagagatgc aaaaacattc gctggtgttg tggcccttcc
    64261 acggtgtgtt cggttccgga ccgacgctgg atgaagcctt tggtttaatc gacaccgcag
    64321 aaaaatcagc acaagtatta gtgaaggttt attcgatggg cggcatgaaa cagaccatca
    64381 gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt tacaccacta gccagtgcgc
    64441 tggcgctgta aggagcaaac atgatccgca aagcctttgt catgcaggta aaccccgacg
    64501 cccacgaaga gtatcagcgt cggcataatc ccatctggcc ggaactggaa gcagtgttga
    64561 aatctcacgg tgtgcataac tacgccattt acctcgacaa agcgcgtaat ctgctgtttg
    64621 ccatggtaga gattgaatct gaagaacggt ggaatgcggt agccagtact gatgtttgcc
    64681 aacgttggtg gaaatatatg accgatgtta tgcccgctaa cccagataac agcccggtga
    64741 gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc caatgtgccg gagatgcctc
    64801 cggcacaata ttttaaattt cgatttgaaa aattaaacat attacatctc ccggtaagtt
    64861 atatttccct gatacattgt gagtaaatca caaaaataat gaataaccca ttaatgattc
    64921 atgtggttta tttaaataac cattatgtgc attactccgc aaatctgacc tttcactctg
    64981 tctcaaattg tcattgttta ccgacatatc gcctctcatc acttaatcgc tccaccatta
    65041 caattgatta aaatatattt tatggagtcc gattaatggc agctcttact gcaagctgta
    65101 ttgacctgaa tattcagggc aatggcgctt attccgttct ga
//
LOCUS       DAETPZ010000012        61097 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.012, whole genome shotgun sequence.
ACCESSION   DAETPZ010000012 DAETPZ010000000
VERSION     DAETPZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 61097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.012"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(192..593)
                     /gene="pemK"
                     /locus_tag="K0W59_000868"
     CDS             complement(192..593)
                     /gene="pemK"
                     /locus_tag="K0W59_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK"
                     /protein_id="HBI3022010.1"
                     /translation="MLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQG
                     TRPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRT
                     IDMKARGGKRLERVPETIMNEVLGRLSTILT"
     gene            complement(526..783)
                     /gene="pemI"
                     /locus_tag="K0W59_000869"
     CDS             complement(526..783)
                     /gene="pemI"
                     /locus_tag="K0W59_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HBI3022011.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            complement(876..1529)
                     /locus_tag="K0W59_000870"
     CDS             complement(876..1529)
                     /locus_tag="K0W59_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBI3022012.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
     gene            complement(2468..3325)
                     /gene="repA"
                     /locus_tag="K0W59_000871"
     CDS             complement(2468..3325)
                     /gene="repA"
                     /locus_tag="K0W59_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HBI3022013.1"
                     /translation="MTDLHQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(3318..3392)
                     /gene="tap"
                     /locus_tag="K0W59_000872"
     CDS             complement(3318..3392)
                     /gene="tap"
                     /locus_tag="K0W59_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HBI3022014.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            complement(3630..3884)
                     /gene="repA"
                     /locus_tag="K0W59_000873"
     CDS             complement(3630..3884)
                     /gene="repA"
                     /locus_tag="K0W59_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HBI3022015.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGNPLSDAEKQRLSVARKRASFKEV
                     KVFLEPKYKAMLMQMCHEDGLTQAEVLTALIKSEAQKRCM"
     gene            complement(4124..4714)
                     /locus_tag="K0W59_000874"
     CDS             complement(4124..4714)
                     /locus_tag="K0W59_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBI3022016.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            complement(4752..4961)
                     /gene="hha"
                     /locus_tag="K0W59_000875"
     CDS             complement(4752..4961)
                     /gene="hha"
                     /locus_tag="K0W59_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBI3022017.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            complement(5007..5468)
                     /locus_tag="K0W59_000876"
     CDS             complement(5007..5468)
                     /locus_tag="K0W59_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HBI3022018.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLINIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            complement(5713..5925)
                     /locus_tag="K0W59_000877"
     CDS             complement(5713..5925)
                     /locus_tag="K0W59_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HBI3022019.1"
                     /translation="MNGTGRDSQNSGVWRYRQVGRRAVRLEVRGDWPGAAEAWLRAAQ
                     SAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            complement(6054..6614)
                     /locus_tag="K0W59_000878"
     CDS             complement(6054..6614)
                     /locus_tag="K0W59_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HBI3022020.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELTAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEV
                     YAAERLDKIRRQNRIKAELQAVLDEQ"
     gene            complement(6717..7577)
                     /locus_tag="K0W59_000879"
     CDS             complement(6717..7577)
                     /locus_tag="K0W59_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI3022021.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLIVIAGQDSVNPPEQGKALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            complement(7636..8382)
                     /gene="traX"
                     /locus_tag="K0W59_000880"
     CDS             complement(7636..8382)
                     /gene="traX"
                     /locus_tag="K0W59_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HBI3022022.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSYRLYRAEDRAERLALLACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            complement(8402..13672)
                     /gene="traI"
                     /locus_tag="K0W59_000881"
     CDS             complement(8402..13672)
                     /gene="traI"
                     /locus_tag="K0W59_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HBI3022023.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQEGMVFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYDRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISLQ
                     KTGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGETFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREIAGQERDRADITEREAALPESVLRESQREQEAVREV
                     ARENLLQERLQQIERDMVRDLQKEKTLGGD"
     gene            complement(13672..15897)
                     /gene="traD"
                     /locus_tag="K0W59_000882"
     CDS             complement(13672..15897)
                     /gene="traD"
                     /locus_tag="K0W59_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HBI3022024.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTSLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGDPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQAEQPQQPQQ
                     PQQPQQPQQPQQPQQPQQPQQPQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEM
                     EQQLPPGISESGEVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDD
                     F"
     gene            complement(15948..16685)
                     /locus_tag="K0W59_000883"
     CDS             complement(15948..16685)
                     /locus_tag="K0W59_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022025.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGHHHRHTGYTWSVSVN"
     gene            complement(16888..17619)
                     /locus_tag="K0W59_000884"
     CDS             complement(16888..17619)
                     /locus_tag="K0W59_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HBI3022026.1"
                     /translation="MKMKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(17668..18153)
                     /locus_tag="K0W59_000885"
     CDS             complement(17668..18153)
                     /locus_tag="K0W59_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HBI3022027.1"
                     /translation="MIMRCLTHITLVTVIQFIACYLASWGNAETIFMLFFIVLWQGLF
                     IWLFSQIRKKRNVSDEFKFSKGVWYITMPVSSLLSPLLSLMVFIIGTLYELRRVSGCV
                     SVREWMQSQVNEQTNEDLHLDFDNMDFHRTNPATGLPMSGIGVDIGGNSYGYSSKRYD
                     E"
     gene            complement(18169..20991)
                     /gene="traG"
                     /locus_tag="K0W59_000886"
     CDS             complement(18169..20991)
                     /gene="traG"
                     /locus_tag="K0W59_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HBI3022028.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVQVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDSNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASHIMRQNV
                     TMNALKEGITSNAARNGDTASLVSLATTSSMEKQRLAHVSIGHVLMRNLPMVQTILVG
                     ITIGIFPLLVLAAVFNRLTLSVLRGYVFALMWFQTWPLLYAILNSAMTFYAKQNGAPV
                     VLSELSQIQLKYSDLASTAGYISVMIPPLSWMMVKGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNSLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASRSTRASAGMY
                     GDAHAEWGVKPKILGVGGGLGVRGGGRAGIDWEDNDAHTASSNTQSSHNARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIDQRTQDSNIRND
                     VKHQVDNMVTEYKGNIGDTQNSVRGEENIVRGQYSELQNHHKTEALSQNNKYNEEKSA
                     QERMPGADSPQELMKRAKEYQDKYKQ"
     gene            complement(20988..22361)
                     /locus_tag="K0W59_000887"
     CDS             complement(20988..22361)
                     /locus_tag="K0W59_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HBI3022029.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQATVPEIKTAKDFLQKMASDINSMNLSSCQVAQGIVGG
                     LFPQTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNI
                     NIIWDSLSKNRMLDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKVYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDDREKGF
                     ISSTTVPVFKYLIDPQMLGVSSSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QTVLDSVLENLNQAQQQIAVFQGQVKVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            complement(22348..22773)
                     /gene="trbF"
                     /locus_tag="K0W59_000888"
     CDS             complement(22348..22773)
                     /gene="trbF"
                     /locus_tag="K0W59_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HBI3022030.1"
                     /translation="MNHRKYQRKLIMKEKRNDAELKNRKTKRDYDYERRVSDIYFDLF
                     FVFVAAGTFLWVIMHSIFDACIDSWKADPELNNFRYMWNILMYVIPYTLWAFAGGFLI
                     VYVRNPLNELINGGIRIFRLKRRMRREKKLREGGNNASH"
     gene            complement(22721..23068)
                     /gene="trbJ"
                     /locus_tag="K0W59_000889"
     CDS             complement(22721..23068)
                     /gene="trbJ"
                     /locus_tag="K0W59_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HBI3022031.1"
                     /translation="MPPVLWRGWIPFCRCTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMNGLNESS
                     EISEETYNERKKK"
     gene            complement(22998..23543)
                     /gene="trbB"
                     /locus_tag="K0W59_000890"
     CDS             complement(22998..23543)
                     /gene="trbB"
                     /locus_tag="K0W59_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HBI3022032.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQMYGEEKGTK"
     gene            complement(23530..23814)
                     /gene="traQ"
                     /locus_tag="K0W59_000891"
     CDS             complement(23530..23814)
                     /gene="traQ"
                     /locus_tag="K0W59_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HBI3022033.1"
                     /translation="MISKYRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            complement(23933..24277)
                     /gene="trbA"
                     /locus_tag="K0W59_000892"
     CDS             complement(23933..24277)
                     /gene="trbA"
                     /locus_tag="K0W59_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HBI3022034.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSSAWSG
                     VMWFFIRGLMTG"
     gene            complement(24291..25034)
                     /gene="traF"
                     /locus_tag="K0W59_000893"
     CDS             complement(24291..25034)
                     /gene="traF"
                     /locus_tag="K0W59_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HBI3022035.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(25027..25284)
                     /gene="trbE"
                     /locus_tag="K0W59_000894"
     CDS             complement(25027..25284)
                     /gene="trbE"
                     /locus_tag="K0W59_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HBI3022036.1"
                     /translation="MKVIFTSNRLVDFLIRLSITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            complement(25311..27161)
                     /gene="traN"
                     /locus_tag="K0W59_000895"
     CDS             complement(25311..27161)
                     /gene="traN"
                     /locus_tag="K0W59_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HBI3022037.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFARQIQGQGTGSIQG
                     FKPQESIPSYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEINRSEFTNYTCERDTMVEEYCTRT
                     ASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFL
                     NSRYTFMNTTFNVGLANGQSDTYPLSGATGLQVTQGQVLTGSGCTANGNCLPHGNGDR
                     KVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLTGTECSEPGGTKT
                     GVMEGKPWNITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLH
                     EYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKSSDFGEAVSQLAALAAAGKDVA
                     ALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLT
                     VSVGEFCSKKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKHPDCRGIT
                     VDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(27158..27796)
                     /gene="trbC"
                     /locus_tag="K0W59_000896"
     CDS             complement(27158..27796)
                     /gene="trbC"
                     /locus_tag="K0W59_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HBI3022038.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            complement(27805..28110)
                     /locus_tag="K0W59_000897"
     CDS             complement(27805..28110)
                     /locus_tag="K0W59_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022039.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVMVPVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCAFMAVFLNYLFSDEFMPVNSRK"
     gene            complement(28140..29132)
                     /locus_tag="K0W59_000898"
     CDS             complement(28140..29132)
                     /locus_tag="K0W59_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HBI3022040.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSVKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(29129..29761)
                     /gene="traW"
                     /locus_tag="K0W59_000899"
     CDS             complement(29129..29761)
                     /gene="traW"
                     /locus_tag="K0W59_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HBI3022041.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(29758..30144)
                     /gene="trbI"
                     /locus_tag="K0W59_000900"
     CDS             complement(29758..30144)
                     /gene="trbI"
                     /locus_tag="K0W59_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HBI3022042.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(30141..32771)
                     /gene="traC"
                     /locus_tag="K0W59_000901"
     CDS             complement(30141..32771)
                     /gene="traC"
                     /locus_tag="K0W59_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HBI3022043.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(32897..33244)
                     /locus_tag="K0W59_000902"
     CDS             complement(32897..33244)
                     /locus_tag="K0W59_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022044.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            complement(33272..33490)
                     /locus_tag="K0W59_000903"
     CDS             complement(33272..33490)
                     /locus_tag="K0W59_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022045.1"
                     /translation="MHTFIFLVLIKNINGGGSWNVTPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPLNNVMETLNGGGQ"
     gene            complement(33571..34044)
                     /locus_tag="K0W59_000904"
     CDS             complement(33571..34044)
                     /locus_tag="K0W59_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022046.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNQINNILAYCACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDVR
                     QELLNRLLSGKKLTGRDEQDIRRLWQEKVDAMQESATRQREQDTIRKFTEEKKSE"
     gene            complement(34037..34450)
                     /locus_tag="K0W59_000905"
     CDS             complement(34037..34450)
                     /locus_tag="K0W59_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022047.1"
                     /translation="MHKSENEKIDFILDEHEQIFRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQETQRKHYA"
     gene            complement(34443..34664)
                     /gene="traR"
                     /locus_tag="K0W59_000906"
     CDS             complement(34443..34664)
                     /gene="traR"
                     /locus_tag="K0W59_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HBI3022048.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(34799..35314)
                     /gene="traV"
                     /locus_tag="K0W59_000907"
     CDS             complement(34799..35314)
                     /gene="traV"
                     /locus_tag="K0W59_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HBI3022049.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNSDFECDATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTTSVQAVAATTPSRSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVAPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(35314..35664)
                     /locus_tag="K0W59_000908"
     CDS             complement(35314..35664)
                     /locus_tag="K0W59_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HBI3022050.1"
                     /translation="MTEQTEATEIKMNMRNINVITARAVPDKNVSDDFMHAVLSNCTT
                     RIALPAPKKFSSESLPHNFNMAAVGVMKKGMSMSLFSSPGNTVVSGQPCAGKTNLTVE
                     LISAVVESNKKGEG"
     gene            complement(35618..36205)
                     /locus_tag="K0W59_000909"
     CDS             complement(35618..36205)
                     /locus_tag="K0W59_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HBI3022051.1"
                     /translation="MPARRAEMAGDALTFRVAFVVRWLLWVVRFVVIWPLATMALMAL
                     FVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAAD
                     AADYVRETDRSLMQLVTAFWATLALMYMSFAAGTGKYPMWPGKMKCVRVGTADEHLKE
                     VYTEDASLPEKIRKCPVYFHDDRTNRSNGDKNEHA"
     gene            complement(36174..37625)
                     /locus_tag="K0W59_000910"
     CDS             complement(36174..37625)
                     /locus_tag="K0W59_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HBI3022052.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGVLYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALEQSQSTAEEGNPESPVPNPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGDKNPLGGDAGKEGGNGG"
     gene            complement(37625..38353)
                     /gene="traK"
                     /locus_tag="K0W59_000911"
     CDS             complement(37625..38353)
                     /gene="traK"
                     /locus_tag="K0W59_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HBI3022053.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPAVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADSVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(38340..38906)
                     /gene="traE"
                     /locus_tag="K0W59_000912"
     CDS             complement(38340..38906)
                     /gene="traE"
                     /locus_tag="K0W59_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HBI3022054.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNQMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(38928..39239)
                     /gene="traL"
                     /locus_tag="K0W59_000913"
     CDS             complement(38928..39239)
                     /gene="traL"
                     /locus_tag="K0W59_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HBI3022055.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGIIT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(39254..39616)
                     /gene="traA"
                     /locus_tag="K0W59_000914"
     CDS             complement(39254..39616)
                     /gene="traA"
                     /locus_tag="K0W59_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HBI3022056.1"
                     /translation="MNTVLSVQGASAPVEKKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGTDLMAKGQETVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLV
                     GFAILSVFIAVGMSVAGF"
     gene            complement(39650..39877)
                     /gene="traY"
                     /locus_tag="K0W59_000915"
     CDS             complement(39650..39877)
                     /gene="traY"
                     /locus_tag="K0W59_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HBI3022057.1"
                     /translation="MRRRNARGGISRTVSVYLDEDTNNRLIRAKDRSGRSKTIEVQIR
                     LRDHLKRFPDFYNEEIFREVIEENESTFKEL"
     gene            complement(39971..40657)
                     /locus_tag="K0W59_000916"
     CDS             complement(39971..40657)
                     /locus_tag="K0W59_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBI3022058.1"
                     /translation="MCALDRRERPLNSQSVNKYILNVQNIYRNSPVPVCVRNNNRKIL
                     YANGAFIELFSREDKPLSGESYIRLQVEIFLSSLELECQALGHGSAFCRRFNFHGEIY
                     QIRMENVSFYNDESVVLWQINPFPDYPFFALNHSGSNTNTSDKLTIWNDLSPGTLVVF
                     SFYMLGVGHATIARELGITDRASEDRIKPVKRKIKGFFEHFDLFRVSCIYKGEIDSLL
                     SIIREFYGVK"
     gene            complement(40851..41234)
                     /locus_tag="K0W59_000917"
     CDS             complement(40851..41234)
                     /locus_tag="K0W59_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HBI3022059.1"
                     /translation="MAKVQAYVSDEIVYKINKIVERRRAEGAKSTDVSFSSISTMLLE
                     LGLRVHEAQMERKESAFNQAEFNKVLLECAVKTQSTVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIRDKVSSEMERFFPENDEE"
     gene            41577..42167
                     /locus_tag="K0W59_000918"
     CDS             41577..42167
                     /locus_tag="K0W59_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBI3022060.1"
                     /translation="MVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFINGIC
                     EAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQHFNEL
                     ARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQRRLA
                     YASEVYRIYTWIKSSKGIRVPTTKKSLSQINSVQKN"
     gene            complement(42464..43285)
                     /locus_tag="K0W59_000919"
     CDS             complement(42464..43285)
                     /locus_tag="K0W59_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBI3022061.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMETMKEIHLNSDEQHLFGRAALMARYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(43396..43692)
                     /locus_tag="K0W59_000920"
     CDS             complement(43396..43692)
                     /locus_tag="K0W59_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022062.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
     gene            complement(44611..44769)
                     /locus_tag="K0W59_000921"
     CDS             complement(44611..44769)
                     /locus_tag="K0W59_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBI3022063.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAA
                     FMAYESGK"
     gene            complement(45049..45768)
                     /locus_tag="K0W59_000922"
     CDS             complement(45049..45768)
                     /locus_tag="K0W59_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HBI3022064.1"
                     /translation="MSARSQALVPLSAEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELRGNRSQWLYAVDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            complement(45765..46199)
                     /gene="psiB"
                     /locus_tag="K0W59_000923"
     CDS             complement(45765..46199)
                     /gene="psiB"
                     /locus_tag="K0W59_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HBI3022065.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            complement(46268..48291)
                     /locus_tag="K0W59_000924"
                     /pseudo
     CDS             complement(46268..48291)
                     /locus_tag="K0W59_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
     gene            complement(48352..48585)
                     /locus_tag="K0W59_000925"
     CDS             complement(48352..48585)
                     /locus_tag="K0W59_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HBI3022066.1"
                     /translation="MSEYFRILQGLPDGPFTRKQAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGTMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            complement(48643..49170)
                     /locus_tag="K0W59_000926"
     CDS             complement(48643..49170)
                     /locus_tag="K0W59_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBI3022067.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMKEQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQVQPQPQQNGQSQSADATKKGSAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            complement(50013..50576)
                     /locus_tag="K0W59_000927"
     CDS             complement(50013..50576)
                     /locus_tag="K0W59_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBI3022068.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVSPAPICDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQSVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(50623..51984)
                     /locus_tag="K0W59_000928"
     CDS             complement(50623..51984)
                     /locus_tag="K0W59_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HBI3022069.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPRQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVEEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(52036..52266)
                     /locus_tag="K0W59_000929"
     CDS             complement(52036..52266)
                     /locus_tag="K0W59_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022070.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            complement(53304..53495)
                     /locus_tag="K0W59_000930"
     CDS             complement(53304..53495)
                     /locus_tag="K0W59_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022071.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSINR"
     gene            complement(53492..53914)
                     /locus_tag="K0W59_000931"
     CDS             complement(53492..53914)
                     /locus_tag="K0W59_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HBI3022072.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFAHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(53961..54386)
                     /locus_tag="K0W59_000932"
     CDS             complement(53961..54386)
                     /locus_tag="K0W59_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBI3022073.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(54804..55580)
                     /locus_tag="K0W59_000933"
     CDS             complement(54804..55580)
                     /locus_tag="K0W59_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022074.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(55631..56065)
                     /locus_tag="K0W59_000934"
     CDS             complement(55631..56065)
                     /locus_tag="K0W59_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HBI3022075.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMEISLT
                     GHEIRTVLARLEDIPEDQRIESGISSAAVMEIIRNESENRLVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQEVVNQARTLLKNNTHEND"
     gene            complement(56079..56300)
                     /locus_tag="K0W59_000935"
     CDS             complement(56079..56300)
                     /locus_tag="K0W59_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022076.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            complement(56301..56984)
                     /locus_tag="K0W59_000936"
     CDS             complement(56301..56984)
                     /locus_tag="K0W59_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBI3022077.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(57061..57354)
                     /locus_tag="K0W59_000937"
     CDS             complement(57061..57354)
                     /locus_tag="K0W59_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022078.1"
                     /translation="MFLGFVWRVTKDVAGLLFLLRRLTRRGRNAACGQCPASGRARDG
                     RFYRSRLALVTVYASPSPFSDERPSSRFRGISSPSKRRRLRYSTVGLTRYRTR"
     gene            57501..58463
                     /gene="parM/stbA"
                     /locus_tag="K0W59_000938"
     CDS             57501..58463
                     /gene="parM/stbA"
                     /locus_tag="K0W59_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HBI3022079.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            58466..58816
                     /locus_tag="K0W59_000939"
     CDS             58466..58816
                     /locus_tag="K0W59_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HBI3022080.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRTPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQK
                     SDEETKKNAMKLIN"
     gene            complement(58939..59220)
                     /locus_tag="K0W59_000940"
     CDS             complement(58939..59220)
                     /locus_tag="K0W59_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022081.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSKSVAEMFHNAAWGVLSLW
                     FELVTKIDLDIHKKNRYASYDFRRKIEMQHEEFQKMTEREQVPLLKLPE"
     gene            complement(59348..59582)
                     /locus_tag="K0W59_000941"
                     /pseudo
     CDS             complement(59348..59582)
                     /locus_tag="K0W59_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421253.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(61031..>61097)
                     /locus_tag="K0W59_000942"
     CDS             complement(61031..>61097)
                     /locus_tag="K0W59_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3022082.1"
                     /translation="SKSVELHDKVIGHYLNIKHYQ"
BASE COUNT        14475 a        17038 c        14494 g        15090 t
ORIGIN      
        1 aacttcaaaa ctgcgcaaaa cacgcaaata cgcacaagaa agtagggctg cgcttggaat
       61 tcggaaaata aaccacatca aaactacttt ttagccaaat aacgtttggg aatcaccaga
      121 atggtgggac aacagcggtt ttagtgccct aaatcgtacg ttttcatcca gttgcccctc
      181 aaaccccatg ttcaagtcag aatagtggac aggcggccaa gaacttcgtt catgatagtc
      241 tccggaaccc gttcgagtcg ttttccgccc cgtgctttca tatcaattgt ccggggttga
      301 tcgcaacgta caacacctgt ggtacgtatg ccaacaccat ccaacgacac cgcaaagccg
      361 gcagtgcggg caaaattgcc tccgctggtt acgggcacaa caacaggcag gcgggtcacg
      421 cgattaaagg ccgccggtgt gacaatcagc accggccgcg ttccctgctg ctcatgacct
      481 gcggtaggat caagcgagac aagccagatt tcccctcttt ccatgtcaga tttcctcctg
      541 accagtcgcc ggtgcatcca gccattctcg ttcttcagct gatatttcag cattcggatc
      601 acactgtgcc agtagctcag ccagtgaata ttgcgggcgt ctgtacggct caacaatcag
      661 ccggccatta tcaatgacca tgccaacttc attatctgtg cccagagaca gcgcattcag
      721 cagtgccggt gggacggtca gcataactga gccgccaacc ctcttcagtc gggtggtatg
      781 cattcttcac ctccataaaa gttatattta aatataacat ccactaaaaa aacacaccag
      841 gctttaacgc acaatgttta ataaaaatat aactttcaac caaacagtaa acccagcgtg
      901 gttgcttcca tatgcagcaa taccggcagc agcaggccac cacttctgat cctggccact
      961 gatgtaatca accccaccag gaacagttct gccagtgtca gcaggttctg atactggctg
     1021 tgcgcggcga cgaacaacaa cgacgttatc agcgccccca gccacatcgt ccagcagtac
     1081 cgtgaacgga agacgttcag cataatcccc cggaacagcg tttcctcatt caacggggca
     1141 aggataaaga tggtcagcaa cgtcaggatc acgtcaggta tggacttatc ggcaaaaagt
     1201 ttcgtcataa atggctcagc aggcagagcc agcgccttac cgagcagaaa tacaccgaca
     1261 tacaccacgg ccatcgcacc gaccagccac ggtacgccaa cgttgcgcag ctgaccaacg
     1321 accggtagcg gagctatcca acggcggtat accaggaaaa cacacagcag gtacatcaga
     1381 acagtaccat gactgaagaa caaatagttt tttcctgatc cataaagcag aacggcctgc
     1441 tccatgacaa atctggctcc ccaactaatg ccccatgcag ccagcataac cagcataaac
     1501 tgcagatatt gattacgtgt ttgaatcatt gcatcgcctg taaattttta acttgtccta
     1561 tttttgtcat taccacgtat atacacatgt ataacaattc agatatcgtt accaggatat
     1621 gccgcatcag cggcatggaa ggcggcactc tgttgtttca tatgatacag gagtaaaacc
     1681 gccgaagccc ggcgtaagcc ggtactgatt gatagatttc accttaccca tccccagccc
     1741 tgccagacca tacccgcttt cagccatgag agagcttctg tgcgcggtcg gagtggtccc
     1801 gacgagggtt tacccgaagt cggggcgtat ctccgcgtta gcgggccgtg agggccgctt
     1861 acgagcgtgt actgagaact tccagcgaga agactgacag cgatgaagat gtagttacaa
     1921 cattcataat taaaagcgac tctgttccgg ccctttgggc cggggcgggg ccgcttttca
     1981 gttatgaggg aggggctttg tggtttcggt tctgcgctgg accggggttt ttctggaggt
     2041 tgtttttgtg tgttgtaact aaagtggctc cggtcggggc ccgccgcttg cggtgggagg
     2101 tgcatatctg tctgtccaca ggacaggcag tgaataggtt ttctttttaa atgaatgtaa
     2161 ttaagtagtt taaaggagat ataaacaggt gtttaaaaga tacattgcac cctgtaagac
     2221 tggcggctgg cgctttatga catgaacggt tgtaacctta tggggaagtc ccttgcagtt
     2281 aaatgtggat aagcaaaatt ccccgtctgt gaggcgtgtt ttgtatcgaa aacagggggg
     2341 gccggatgca ccagaagctg gatgatgagg ttgttttttg tatgtggtgc tgattttttg
     2401 tgcgctgtcg ggcttcagac gtgcggaagc ctccggcgca ggccggatta ttcagaggag
     2461 gtcactttca gggggaagct gtggccagcc ggctgtaatt acggttacgt gacagaatca
     2521 tgcgctcctt cacacgacgc tcaacttcgc gttttaccgc ctcacgattg gcagtgaagc
     2581 gcccttccgc gatttcgcgc gtcagctgcc gtttcaccag ggtgacaata tcctgacgtt
     2641 ccctgtccgc atcacgacgc gcacgggcac gctttattcc ccgggactta agctctgtct
     2701 gataactgcg aaaacgctca cgaacaaaac gccaggcttt cgctatcagt tcatccatgc
     2761 ccagggtatc cagcccctgc tttttgcgtt gtttgttttc ccataccaca cggctgcggc
     2821 gcgcggcggc cactgcctcc tctgatacat cgagggcagc aaacagtgca gatgtgaacg
     2881 tgatatcggt cggaatgtag cacccgataa gcgggtcata ttccgtctgg taggtaatca
     2941 gtcccagctc tgacaggaac gtcagggccc gggtggcacg ggtgatggag agttttccgg
     3001 cagcagactc cgtcgccagt ccgcactcaa tggccagcgt ggtgatggag cactggacgc
     3061 ggttggccag cgggtcatag tggaaacaca gcccctgcag gagcgcatca atagcccgtc
     3121 gacgcagcac tggtggcatg cgtcgacgca gaccacgcga acgggcatgc gccacatgaa
     3181 tggcgaaatc aaaacgggaa gtgaagccca ccgccttttc catcagtttt tcgcagaact
     3241 tcagcgttcc tgcaccttca cggggtgtaa acaccggatt cgggttcttt acctggcggt
     3301 aatacgtttg gtgaagatca gtcacaccat cctgcactta caatgcgcag aaggagcgag
     3361 cacagaaaga agtcttgaac ttttccgggc atataactat actccccgca tagctgaatt
     3421 gttggctata cggtttaagt gggccccggt aatcttttcg tactcgccaa agttgaagaa
     3481 gattatcggg gtttttgctt ttctggctcc tgtaaatcca catcagaacc agttccttgc
     3541 caccttacgg cgtggccagc cacaaaattc cttaaacgat cagtaatcta gcactaatct
     3601 tctgaacact caagaatgta agcccatcat cacatgcatc gtttttgcgc ttcacttttt
     3661 atcagtgcgg tcagaacttc agcctgagtc agaccatctt catgacacat ttgcatgagc
     3721 atggccttat actttggttc aagaaatact tttacttcct tgaacgaagc tcttttacgg
     3781 gccactgata atctttgttt ctctgcatca gaaagcggat tcccctttct gtatgctcgt
     3841 tttgcgccag atgaggaagt cactgcattt tctgtctgcg acatctcgcc tcctcaatac
     3901 tcaaacaggg atcgtttcgc agaggatact acagtttttt gaaatcagcg acttgagaat
     3961 tgtgacgaag atctgtgttg gcgcacaaat caacggggat tactgtcgtt taatgtgatt
     4021 taaactgtga aatagtatgg ttttcagtta ttgaaacgcc gggagcgggg aaaacttgct
     4081 ttttcccgtt tccggggttg gacaactgag caacgcgaag gcgtcagcta cgatgttccg
     4141 gggactgctg atcagcccct gttgtattca gccattctcc ggtcatctgc agcagttttc
     4201 tggcatcgtg gcttctgagc aacggaatac cagcctgcct cagaacctct tccagaagaa
     4261 tatcccggcg tctgcgttcc ggtcgtaaat ggctggcatc atccagctct actgctgcaa
     4321 caatactgaa cgaacgccgc tcaacgataa ccacatcaac atgccactga gacaccatcc
     4381 tgaataactg ccaccactgg cgtgatcgtg gccggatatt cccgttcagc tggacgatat
     4441 ccgcgacccg tacctgcgga cacagatacc accgcttcat atccacaatt ctgaacagtc
     4501 ctttcataaa actgacttcc ctttctgtca gcagcgtctt ctgacgttgc cagtattcac
     4561 cgccggacag tattcgctct gcatcaccgg tccgtatacc tgccgcatgc aattgcatca
     4621 gcaaaggaga atgctgattt acacttctga ggataatccg gataagtaac gctgcaataa
     4681 tcagaacaat aagaataaaa ataatcagct tcattgttta tctgctcctg gtaattaatt
     4741 atgctgaatt attaccttac atatttccat atctcttttg gtatcctgtc atataacttt
     4801 ccggtgatta attccgctaa ccggtggtct gctgctgaat taaatgattc acgttcactg
     4861 tttaataaac tgtctctgtt tttatgaata attttttcca gcgtattgac tgatgaacat
     4921 cgccgtaact gatacagcca ttcctgtttt gttttcgcca tttttattcc cgctgagaac
     4981 agatgaatga taagattgcc atcgctttat ttacggtgtc tccatatcca ggggggaacc
     5041 ggatcagcat ctgaccagag cccacgtttt tgttgtctgg cttcgttctg aaggacaggc
     5101 agcacgggat ctgtgttgta ctgctcatat acccaggcag ccccggcccg gaccataaac
     5161 tgattaacat tcattccgtc tggtgcataa acctgcccga gaatacgacc ataacgatct
     5221 cgctgaaaat atgtgacggt aaccgttttt cctgcaacca gtgatttcat catgtccgtt
     5281 gaccaccggc cataatcctg tttcttttcc ggtgcatcaa tatttatcag cctgatccgc
     5341 actgccttcc gtgagtccat gacctcgatg gtatcaccgt ccagtacacg aacaacccgt
     5401 ccatgaatat ctgcacacaa taccggccat gaaaagataa ataataccag tggaatatat
     5461 ttcctcatgt tattctccat atcagataaa tggcacctgt atttcaggta atcctgaata
     5521 tcgcagcaca cactgaataa caccttattt tgtgaaatta atcacaaaat ataaaatcac
     5581 gacttaatac ggaaaaaact ctgtgctact cagaactgtc cggtcatact tcccgaccgg
     5641 atattaaaaa aagcaaatct catgcattat tcagccttct gaatccctgg agtaatgaag
     5701 gctgcttttt cgttacaccc ttccccggca gcgccggtgg cagtgttcag ccctttttcg
     5761 ggcaaactgc tgccagtctg tccggggagc agactgcgca gcccgtagcc atgcctcagc
     5821 ggctcccggc cagtcccccc tgacttccag ccggacagcc cggcgtccga cctgccggta
     5881 gcgccatacc ccggagtttt gcgaatcacg ccctgttccg ttcattttcc ttcccccttt
     5941 aatccaggcc ggtgaatgtg ttataaattt ttataacctt cttctgccgg gtatggaaga
     6001 aggtttcaga gaagccttat ttttccgcct cctttagtgt gaaggaggct tttttattgt
     6061 tcatcaagca cggcctgaag ttctgccttt atccggttct ggcggcggat tttatccaga
     6121 cgctctgccg catacacttc ttcctcctga gaaatatgct ccgtcacata cccttccgtg
     6181 tcataccggc aggcaccggc tttcatggca cacagatagc tttctgaacg ggtgatggcc
     6241 ttcagcgccc tgcgcagttt tttatgcgag agcgggatat tccgctgcgc cacgtcttcc
     6301 agtaacacgt cccggatacc gcaggccaga agccggggtg tgtcaccgtc aaataatccc
     6361 ggccaccagg gtttcagggt gttcacggct tcatccagtg tgggcaggtt cagataaatg
     6421 gacagcgcct gtctggcctg cgcttttttt gctgtcagct ctgcttcccg ggcagccttc
     6481 tccgccagtt tctgcttttt caccttccat tttggtggcg tggtgacatt gatgatggtt
     6541 ttccggctcc gggtcggtgt ttccccttcc gttttccgtt tcagtgtcag taccggtcgt
     6601 ttctgctctg tcatgattgc cctgaccttt gtgaaaatat gtcctccagg tcgttgaaac
     6661 tactcactat gatattaagc tgacctcaac gacctctgac atattaatac cgacaattaa
     6721 agatgttttt taaaccattg cgtctgtact gccgccacac gttcaaaaaa cgcgccttca
     6781 taaatatcat aatggcatgc gtcagcttcc tcgtataatt ccttagtacc agaagcaaca
     6841 gcatcatata aggcttttcc ctgttctggt ggattaacac tatcctggcc ggcaataaca
     6901 atcaaaactg ggcactgtac tttggctgcg ctctctgccg gtttatattg caacgtctcc
     6961 ataaccgtaa ggaaaggtat ttttatatcc atttcaggat actgaccttt taccttctcg
     7021 aagaatacct ttgattcatt atcactcagt acccgggtaa cccccacaaa catctctttc
     7081 ccggtgtttt tcttcttctc tgccatttta ttcagcgtgg acagaaaaga cgctctctcc
     7141 gattcattca tctctccggt aaccagaaca tcaccgtcag caaaagccag ctggctgaca
     7201 atacatttta cgcgttggtc ctgcgcagct gcactgaaga catgcccccc tcccagcgat
     7261 gttccccata acccaatacg ctgattatca atacattcct gcttttctgc ccaattgata
     7321 actgaaataa tatcttcggt ctgcattgca ggaaccagac gccctcgttc tccatcactc
     7381 tcaccgaatc ctcgataatc aaaagtaatc gttgcgaatc ctgcttctgt gaaggcattc
     7441 gcaaagcaag ggagcagaac attcctgata ccgcaaaacc catggcataa gataatcaaa
     7501 ggatgtttta tatttccttc aggaacacga aatgtcaggg caataccttc agagagtttg
     7561 tgatccgtaa ttttcataaa tatcctgtgt tatgttgttt attaataatc agaatcagcg
     7621 tcagtatcat accagtcaca acgccagaac gcccagcaca gccagatgac aggcgtagaa
     7681 taccgggaaa aaatccccgg gccagaaacg gggtaatgat tttcctgcac acgacaccag
     7741 accaacggtc agcaccgtca ttacaagacc ggccaccgcc gctgcatcac tggaggcaag
     7801 gttaagtgcc ggaataacgg ccagcaggca tgcaagcagc gccagacgtt ccgctctgtc
     7861 ttccgcccgg tacagtcggt aactgaccgc cagcatcagc agcccggcaa tgccgtaact
     7921 ggtgccggac aaaggccccc acagggccat cagaagaatg gccgctgctg tacgccaccc
     7981 actgcgcgtt tcacaccacg tcagcacctg tgccgccact gcaaaggcaa acaggatatt
     8041 cccctcatac cagggaaagc cggccagata atatgcgaac tgggcaataa ttccccatcc
     8101 ccacagccgg ttaatggccg gttgccggat atgcgcatga cgggacagat tcagccccca
     8161 caccagggca aacagcggaa aggctcctcg cccggccaga aacatccatt cctgcttcag
     8221 ctggaatatc agattgatgt ggtccagtac catcagcacc agcgccaccg ttttgatgat
     8281 atcccgctgt ccgggtgacc agaccagaaa cgactgcaac caggtatcgg tccgtgcagc
     8341 cagtgaatcg ttacgtgtcg tgttcgtgtt atccgttgtc atcagcgttt atcttcctgt
     8401 atcagtctcc gcccagggtt ttctctttct gcaggtcacg aaccatatcc cgctctatct
     8461 gctgcagacg ctcctgcagc agattttccc gggcaacctc ccggaccgct tcctgctccc
     8521 gttgtgattc acgcagcaca ctctccggca gcgcggcttc ccgttcagtt atgtcagccc
     8581 ggtcacgctc ctgcccggca atttccctga cagcctgctc tgttttgcca tccggcaggt
     8641 caggtttgtt ctctgccatt ttacggacaa tttcatctgc acggcgttcc gtttcacggc
     8701 ggatggcctc ttcagcctgc cgctgtgcca gcacctctgc cgtgaccggt tcgccatttc
     8761 cggctcccgg ctggacgctg ctgtccggga tatcccccca cacgcgacca ccggtgatgg
     8821 caccgggatt ccacggacga ccttcaccgg caattcttac caccacaccg ctgtcaggat
     8881 tatcacgggc aatccggaca ccatcctgca tattatcagc cagcaggctc tctccgttac
     8941 ggctaccctg cagggccacg aactgcgcat ccccgctgcc tttcacccgt ccttcaccgc
     9001 tgaatccccg cagcccgttt ccgtcatccg tggtcagtgg gttcagccag ataccggcgg
     9061 acttgccgtt acggtcaaac gccggcagtg ccacatacgg ctgcggatat ttacgtcccg
     9121 gtgcaataaa ccgtgcaggg ctgtctcccc cggccagccc tgcctgacgg agaacggcac
     9181 ggcctgccgc cacgtcccgc agttcccgtg ccgtactgaa cagccgctgc gcattcatga
     9241 cctcccggtc cggtttcggc tccagcacat cgtgggcagt tcctttctgt acggcattgt
     9301 taatggcatc cgtccagccc tgacggttat cggtgtacac ctgcacatgc tgcttcatac
     9361 gcgacagggc cacgtaggct gactcaaagc cggccatcag tttccggtta ccttccgtgc
     9421 cttcaagcgc gatggcaaag gtttcactcg ccccctgcgc accgtgggcg gtgatggcat
     9481 aagccaggtc aatatgttgc tctgcccgct cctggccggg gcgaatcacc cgggtctgct
     9541 gtccgtctga caacgtgaca ctgtcaccgg aaaccgctgt caccgtccag acgctgttgg
     9601 ccacataacc gcgctcccgg tcactcttcg tgaagcgcat ccggtcgccg gtccccaccc
     9661 ggattttgtc cggggtgtac agtgtgacac cttcagccac cgcctcccgg ggcgaaatca
     9721 gccgcgtgtt accttccgca tcctccaggg ttatcagccc gtcatccttg ctgataccgg
     9781 caatccggtg atacacacta tccaccaggg caagggcatc cgggttattc tcccaggtgg
     9841 agagacgacg cagctccccg tcacgtatat tcgctgtgtt caggacaggc accatgacct
     9901 gctctttccc cagctcaccg gccttttccc gtgcatcatg aatcatgctg ttcagtacgc
     9961 gccggtcctc attcaggtgc gtgacaatca gcgtctgctc ccgtgcttcc ggtgtcctgc
    10021 cggtatagtc gcggacaatg gcttcataca gtgtcatggg gacatccgga aaagtctcac
    10081 ctttcagcat cgccttctgc tgcgcttctg ccagtttcgc ttcctggctg tgactgaact
    10141 ccgtcacgga gtgctccggt gcccatgcgc cctcctgacg gggaacctga gacggtttca
    10201 cactctcaag cccggacagt gccctttcca catcccggtt aatcaggctg tataccgcct
    10261 cccgcagttc cggcgtctga cgcacaatct ccttcatgat gaccacatcg gcagcactgc
    10321 gcgtctgctg gagacggaaa ggctgaccgg gcgcgatggc ctgcagctgg tccgtgtcac
    10381 cactggccac ggcacgaccg ccaccggccg caatcagggc gtatgcccgt gccatgtcgg
    10441 tattgcccac cattgagctc tcatcgagca ggaacagcgt gttgctgaaa tccggcgttt
    10501 ctccgctgcg ctgctgcagc tgcgtgtcat gcagaaagga cgccagcgtc tgtgcatcca
    10561 cgccggcgct gcgcatctca ccgaccgcac ggtgcgtggg ccccagcccc acaactcggg
    10621 gacgctcact cgccggcagc atgttcacgg ctgacatcac cgcccggaac tgtgtggtct
    10681 tacccacacc ggcataaccc tgtaccaccg tgaaacggtc ggacgtttcc agtatcatac
    10741 gggtggcggc acgctgtccc gacgttaacg tctccatgag ttcgccgggt actctctcca
    10801 tcagcggcgt gaccgcctcc ttaccttcga gaatatggcg aagaatactt ttttctgcct
    10861 catacgacgc acgggaaacc agcaggcctg tgccatagcc ttttgccaca tccacataca
    10921 gtaagtcacc gcgttttatc tgcgcattga tttcccctcc cagttcagta aaaccggttc
    10981 cttctgcagc aaacgacttc gcctctgtca gcaggtccac catgctgaag gccaggtttt
    11041 tactttccag cacaggaagg gccagatgaa tggcctgctg tgccggcgtg tgcagcccgg
    11101 ttttctgcag actgatagcg gtttccagcg atgtctcacc ggcccgcgcc tttatctgct
    11161 cagaaaccac cgtaaaggag ggatggcggg caagtttttc cgcagtacgg gtttcatcca
    11221 gtgaggaata cagccggaca tcacgaccgc tgcgggccag accgttcagg gtggcgttgt
    11281 ccattgccat ctgtgtgacg gaggcaaaca ccgtcgcact gtcactgacg gaatgcccgg
    11341 gcgtttccac ccagccgttc tccagcttca gtgccgtgaa cggtgaatca gccacaggca
    11401 gggtggccgg ttcagcccgc cccggcacaa caaccgtcat cgcatcttca ctgacggatg
    11461 ccacctgcag gcggtcaccg ccggagacgc ggagtccggg aattttccct gtcaccctca
    11521 gtcgctcgcc gtctgccacc ggcatttttt ccggccggaa cagcgaccag ctgctgtcca
    11581 gggaggaaat acgcaccacc tgcgtttcgc cctgcgcatc ccgcagggtc aggctgtgac
    11641 tctgcgccgt cacccggtcg ataacatagc ggtcatgact gcgtgtctcc gggttccact
    11701 gctccatcac catccccggt cggtacatat cccgcagata acggctccgg ctgtccagcc
    11761 agaccggtga cagggcggtc atggtcacct ccgggtgtcc gagcacgccc tgtgttttca
    11821 gctcactgcg aatggcctgt gtcagtatgg cctgttcccg taccccgctg acctgtgcca
    11881 cgctctcttc tccggctttc acgctggccg caaaatctcc ggccagccgg tcatagcgga
    11941 cattccggtc cggttcactg atgatggttg ccggtcgctg ttctccgccc tgccagcgat
    12001 acgtgttcac cccggcatcc ttcatggcca tcagcgcact gcctgtaccg gttcgctgcc
    12061 cgctgtcggt tatcaggacc tgtacgttat gacgtgcggc accatccagc agggttaacg
    12121 tctctttcag ggagagtttt tcgccctggt cgacgataac agtactgccc ggcgtgaaga
    12181 ccatgccctc ctgcagctga cgacgtccgg ttatcagctc accggacagc cgttcatcct
    12241 gcttcaggtt catctgcgag cgacggtcag cagcgataat ctgcacctcc cgcccctgct
    12301 cccgggccat catgaccagt tcagccaccc gctcacgctg cccggctgca ccgccctgcc
    12361 cggacacaat ggccagcgac ggacggtcct gtgccagaac actgaccgca tcgctgtaac
    12421 cagccgtccg ggggacactt ttctccggat gtacggtcac ccggttctgt ttcatgatgt
    12481 cacgactgag tgcccggacg gacagctcat cgagcacatg aattcctgat gtgaacagcc
    12541 ccttctcacg gtcgaggggg ataagctgct cacggctgat ggcctcatcg ataccggcgc
    12601 gtgcccgttc aatcacacca ttttccggcg gcagtatgcc gaccgtcctg gccagcacgt
    12661 ccgtatacgt gaactgcact tttcgttcac ttaatccggc aatcgcctgt gtcaccgcct
    12721 gctgcacatc cggcccgtcc tgtgaagcag gcccgggcgc ctgcgtgcgg atctccgcac
    12781 gctgatccgc cgcgtcacga tatgcccgga tgtcgaaccc ggtttccttc agcgtctgca
    12841 tccattcagc cattctgact tcaggatcga cgtgctgttt ggattttcgc gtatccagtg
    12901 ccgccacatc ccgggatttc agcgaggcgt cttcaccgac ggcctccctg atagcctgtg
    12961 aacgactgga aaaggcctcc accggtacgc ccggcatctc ccacataccg tgcttaccga
    13021 ccacttcggt ttcataaccc agcgcctcaa cgcgctgacg taactcactc tgatatattt
    13081 tcccgaacgc aatccggttc gctagcacgt tctcggaaaa tccggttttc cccactttgt
    13141 cactgctcag tgtcttccac tcgccgttat gctgcgtgac attagccacc actacatgcg
    13201 tgtgtaactg tgggtcctga tcgcgactgg tgtcatggtt aaacagtgcc atcaccagat
    13261 taccggtcag caccgtttct gactgtccgt ccgtcatcac ccgtgtggaa gccagcgcct
    13321 ccacctgacg gacagcaaaa tccacggcct ggttatgtgc atcaataaga cgtttatccc
    13381 ccccgagcat ggccatcatg gagacacttt tgggggcgga gaaggtcagg tcgtagccgg
    13441 gacgatgctt attactgcca tcctgcatgc ggcttaaatc cgccccgtcc ggcagcctgc
    13501 cctccagaag acgggtaaaa acatccttat cgacgctgcc ctgcagccca agctgttcag
    13561 ccccctgacc agcccagcgt tctcccatgc tgcccagcac atagtaatta tccttgtcgg
    13621 tgtaatagtt tccggcactg cctgccgatt taacaacgct gaaactcaac atcagaaatc
    13681 atctcccggc tcaacatcct ccccgcgctc ccggtgcaca ttaatgttca cctcttcacg
    13741 acgctgcata tgctgctgga tgtccggatg attttcctgt tgccatgcct cataagcggc
    13801 catatccacc acttcaccgg attcactgat cccgggtggc agttgctgtt ccatctcttc
    13861 ttccggtttc attttcagct cctgctcgat cccccctgcc ggaacactca cacctgcatc
    13921 tgacttctta tcattgatga cagaagacac tggctgttgt ggctgttgtg gctgttgtgg
    13981 ctgttgtggc tgttgtggct gttgtggctg ttgtggctgt tgtggctgtt gtggctgttg
    14041 tggctgttca gcctgagtca catcttctcc ggaggcaacc tccggttcaa agaggctggc
    14101 catctgacga ccttctgctt cccttgcggc aagtacggca ctcagacggt tctccatttc
    14161 cgggttgatg tcacgcggaa taaactccgg agcgaccttc ggtcgtgcct gatatttcag
    14221 agagagtttg actgccggat acggtccggg cagagtgaca tagcaggtca gatccggcag
    14281 agactgaatg tcggaataac tgaccagcgt ctgacgctcc atatccttac cggtcgatac
    14341 cccgtcacgt accgggtcag caccgtagga atactgctcg ctggctttca ggtgctcttt
    14401 ctcaccaatc tcaccggcag cgaactctgc aatcttatgg ctcggggaac ggaaaaaagc
    14461 acgggtgttc atgacgtcaa acagcgtggc cgccgctttc tcaccgtaga tatcttccag
    14521 ctgggcatag gactggatac caaacacata acagccaccg aacttacggg cttccggcag
    14581 gatctccacc agatccggca gtttgtgtaa cgtgggtaac tcatcacaga aaaaccacac
    14641 acgacggtta cggttttctc ccattgccag cagaccacga atggcaatgg acagccacat
    14701 ggagatcaca ggcttcaggg aggcatgggt gtcggcgttc gacgaaataa acagccagcc
    14761 gtttttctga tcttcccgga caccacgcat ccagtcacgg atggtgaagg gatcaccgtt
    14821 atgctcaatc ccctgcaggt aacggatggc tttcacgtag ttggtcagca cagcacggat
    14881 ggaaatcgcc gttttctcaa ttttctcttc caccaggttg gccgccggtg aattacgcag
    14941 gaaggtacgc agtttttcaa ttttgatgga aagcagtgtg tccaccagtt tgctgtagct
    15001 gcggttgggg tcattacgca tcaggtacgc cgcttccgca aaaatggtac gtcctgaacc
    15061 ctgccagaac gggtcctctt ttgtacccat agggatcagc gtgttcgcgg tattatcaaa
    15121 atccggctgt gtcaggcact ccttccacaa atcccatgcg gcacagcggg catccagcgg
    15181 attcaggatt ttatcgatgg agggatcata gtaactttta acaaattccc ctgaacggtc
    15241 ataaatcacc accatatcac cgcgctgacg ggcatagttg gccagacgac ggataacctc
    15301 cgacttaccg gccccgaccg tgccgtgcag acagaagttc tggatttcgg aatcccggat
    15361 aatcggcagg tcgccaatcc ggatatcgga gtccttgccg tcttttttca gcatccgggc
    15421 aacgtctttc ggattgtctg tcagctgacg accacctgtg acttcatttt cgctctgttg
    15481 tttaccctga cgccccagaa tccaggagac aacaaagaag gtgatcaggc aaataaccag
    15541 ggccacgaca gacgccagaa cgaatgcaga ccatagctgc tctccgcacc agatcatata
    15601 tttatcatgc agtacctggg cagcgttcat ccggaaggtt ttgccgtaat actggatctc
    15661 atagaccggc tgggatttta ttaaatcccg catgccttcc agtgacgtac accaccagta
    15721 aatacagccg ttgataaacg tctgccagct tatttttacc cataagacca gaccaatgag
    15781 gatccagaaa aaaataaaca ggcagtaaag catgatattg gcgatctggc tgaacatgcg
    15841 gatacgcatg gacgcaatct gaccgccctg ggtcatatcc tttgcgttaa aactcatgat
    15901 gaaactccgg gaaaatctgg aacagggcag aaaaggatga tgtggggtta attcacagaa
    15961 acagaccagg tgtaccctgt atgccggtgg tgatgcccgc ctgcagtcct gccggaagag
    16021 accttctctg taacaatgtc agtcaggcgc aatacttcag accagtcact cccatgcgga
    16081 aactggatct gaataaaaag gcgtacccgt tcacggtgat ccagaactgt gtccggttca
    16141 taaaaacggc cactgtcagg agaaacacag gacgttttat tgatgagatc atacttatcg
    16201 gcaggagact gcgatgaaat cgcccaggtg atattctccg cctggtggcc atcaatacga
    16261 ctgctccccg gcgtccatgc aatttcctgc gcactcgcca cgatgatctc cctggaaact
    16321 tcagccagct ttctgggtgt acagaatacc ggtgccagca cacacaggga caacaaatta
    16381 cagctgaccg cctccggttt aagcaaattg ctcccggatt tttcaggaaa agatattttt
    16441 gtcagtattt cagttaattc cttatgcctg agagaattaa taaagtacat gataagcatc
    16501 atgagaaaca atataacaat aagccctccg ataaccacta aaaggtcagt atcaatatta
    16561 ttgctgtaca gatagtcttt agtggtcact gtattatcct gtatcaccgg ggggtattca
    16621 gttacaggct tctcagtaaa ttgattattc tcaccaacat attcagcgat attgtctgat
    16681 gtcataatga ctttctccgg aagatcccgg cttcgccggc aacacttcac ggtcgcacaa
    16741 agaatttgca caaccgctca gtgttttcat atttatttcg gaaacgccgg gaggtgacaa
    16801 aatttatttt gttacatccc ggggttggac aacgagcaac gcgaggcgtc agcaaggagg
    16861 ccggtcagat cagcctcctg acgaggttca gagaatattt gcgattgatt tggccagttg
    16921 gtcttcgaga acaggcttcg cctcttcaaa tttcaggtta accttgttcg catttgaaac
    16981 cacacgggtc tggtatttat gctggttacc tgtttcagta ctggtctgaa ttttcgcacc
    17041 tgatgtgccc tgacgcaggg cggcaacatt atccgttgtc accgttgcct tagtacgctc
    17101 tgcaatctgt acatccgtga tcatggtata gttcacatct tccaccatcg catctgcagc
    17161 catacccacc agaccagcag caaggcctac accgagtgtg gcaccggcag aatttgagtt
    17221 atagccggta ataccggcac ctaacgctgc accaactgct gcgccttcat aaccacggtt
    17281 cagccatccc tgagactccc gcagatccat cttatcggcc ttcagcacat tcgcctgaat
    17341 ccagtagtag gctttatccg gagaagtcac cacctgatat ccttttgctt tcacagcatc
    17401 agcaattttg ccctgcagcc cactcatgtc tttatcagac gtgtttttga tctgcagaaa
    17461 taccgtgcgt tcgctggcgg gttcaagcca gatggtctca ctcatctgag tcttcacctc
    17521 aaggttacgc ttcttgattg ctgtgctcat cgcaccacac cctgaaaggg ccagagtgga
    17581 actgaccagt gcaaccatca tcaatttttt cattttcatc aatgttcctt catattgaat
    17641 aaaacgtcat aatatacaaa ttaagattca ctcatcatat cgttttgaac tataaccata
    17701 tgaattacca ccgatatcca caccaatacc tgacataggt aaccctgttg caggattagt
    17761 acgatgaaaa tccatattat caaaatccag atgcaaatct tcattagttt gttcatttac
    17821 ctgagattgc atccattctc ttacgcttac acaaccacta actcgcctta attcgtatag
    17881 tgtaccaatg ataaaaacca tcaggcttaa taatggagag agtaaggaag atactggcat
    17941 cgttatatac caaacgcctt tactaaactt aaactcatcc gacacattcc gtttttttcg
    18001 tatttgagag aacaaccata taaacagtcc ctgccatagt actatgaaga acagcataaa
    18061 aattgtttcg gcattccccc aactagccag ataacaggcg ataaactgta tcaccgttac
    18121 cagagtaata tgggttaaac atctcattat catctcccaa aaacagcatt attgcttata
    18181 cttatcctga tattcctttg cccttttcat cagttcctgt ggactgtcag ccccgggcat
    18241 tctttcctga gccgactttt cttcattata tttattattc tgagataatg cctctgtttt
    18301 atggtgattc tgcagttcag aatactgtcc tcttacaata ttttcctcgc cacgtacgct
    18361 gttctgagta tcacctatat tccctttata ttctgtgacc atattatcaa cctgatgttt
    18421 tacatcatta cggatattac tgtcctgcgt tctttgatca ataatggcct gatgcccctg
    18481 atggtcagca ataatatcct gactgccacc acccgaaggt acgctctcca ttcctttacc
    18541 aatatcacca cgggattccc gccaggcatt atcaacacca ggctgtacct gttcctgcac
    18601 aaaagaccag gccatcgccc gacgacgttc tgcaatctcc ggtgaactgg tgtttgtcag
    18661 aatagcttct gcatcctgtg gcgcgtgttt cattacatac tgtgcaaact gttgcgacag
    18721 gtcttcactc atctgcccac tcatgctttc agtacgggat gccatttcag catattcatg
    18781 gctgcgggtc atattcgtcg tgtactggtc gtaactctgc ttcgctgaat tcagggctgc
    18841 agacaactga tccacacggg aatcagcatt attgtccgta tggctgccgg actcactgac
    18901 cttgcgactg gtaaaataat cgctggcctc tttaaagtct tgtgtagctc tggcatcgat
    18961 atcatgacga gcattatggc tcgactgagt attactactt gccgtatgcg catcattatc
    19021 ttcccaatca ataccagccc gaccaccacc tctaacgcct aatcctccac caacaccaag
    19081 aattttaggt ttaacccccc attcagcatg agcatctcca tacatcccgg cagaagctct
    19141 cgtacttctg gatgccagtt cctgagtcgc ctgctcatta ctgatattat gcgccttcgc
    19201 atagctttcc actgcactgc gcatacggct ggccatcatg gagtcctgtg cgctcatcgt
    19261 gctgtcagca ccacctgtga cagaatcact gctcccccgg ttagaaccaa actggctgag
    19321 cgagttccag gcactggcaa tactgctgct gaacccatgc agggcgcttt ctgctctgtt
    19381 cgacgcctcc cgggccattt cctgttgtgc cgctgcaatc tgacgcgttg cattgatacc
    19441 gaccggtaac cgggacatcg ccccgctcgc atccatcacc atattaccgt cacgggtctg
    19501 tgtggcagtt gcgccactgc cggtctggta catcatctga ccaaacgacg tggtgctgtt
    19561 ggtgctccag ctgaagccgt tcacgttttc cgtctgcatg ttgccgtagg agtaattacc
    19621 gtcaaccaca ctacctgccg cactggcagt cgggctgata gcagaggaag cgaaatggct
    19681 gtacacgctg gaaaaacctg cgccaaggcc ttttaccatc atccatgaca acgggggtat
    19741 catgacagat atatatccgg cagtggacgc caggtcggag tatttcagct gtatctgaga
    19801 gagttcagag agcacgaccg gtgcaccatt ctgctttgca tagaatgtca tggcactgtt
    19861 cagaatggca tacagcaacg gccaggtctg gaaccacatc agagcaaaca catagcctct
    19921 cagaacagac agcgtcagtc tgttgaagac agccgccagt accaggagcg ggaagatacc
    19981 aatggtaatg cccaccagaa tggtctgcac catcggcagg tttcgcatca gcacatgacc
    20041 aatggagaca tgtgccagac gctgtttctc catcgacgaa gtcgtggcca gactgaccag
    20101 gctggcggta tcaccgttac gggctgcatt actggtgata ccctctttca gggcattcat
    20161 ggtgacgttc tggcgcataa tatgactggc agactggctg gagccgtaga aataactgta
    20221 actgtcactc accagttgcc tgaataacag gtccgggtcc ggtctgccgc caaatatctg
    20281 ctgcacataa taatgccagg tcttgcctcc cgtctttgta tcgagattca gcctgtcttt
    20341 cagcgtgacc gacgcatctt tacaggttat gaaattatta ttgctgtcat aaacgccccg
    20401 tagcgggctg ggccgggaaa aaatcagcgt gtaggggtca gcggaggcca tcaggtcttc
    20461 cagcgtgtat ttgtggttca ggtaaatatc ccccagcacg cagttctgga cgtagtcctg
    20521 aaacagattg atgatttccg gattccggga cagaaaatcg gtgcttttca caatcaggtt
    20581 cgccccgaac agcatgcctg tcttgctgta ggtggcgctg tcaggctggg tgaatatcat
    20641 ctcgtaactg gccaccattg catgcccgat acgggtcgtc agtgaaagtg gcatcgccag
    20701 tccgacaggc acattatcca cccggtgaac ctggaccagg tcactgttat caataatctg
    20761 caccgatgta cggacattaa ccagcaggct gataagcaca aacacggcca cccagcccag
    20821 caaatccatc acgttgtgcc gctgtaccca catcacggcc accgcgagaa cagacaatgt
    20881 gagcgcaatt ttttcgatgg aatcccacgt ccgggtcccc ataaaggcgg caatggcgtt
    20941 caggttattg cgtaaccact caccaccggc aatcacatag acttcattca cagcgtgctc
    21001 cctccgaagt gatagttgtt ctggtaacgg ctgagcatgc gggcggaaag ctgctggcgc
    21061 atatagctca tctgacggtc aacaaccagc aatgcatcct gctggacctt tacctgcccc
    21121 tggaaaaccg caatctgttg ctgtgcctga ttcagattct ccagcacact gtccagcacg
    21181 gtctgtggat agttcccggt ggcaatcatg gcccgggcct gctgaagcag ttcctgaatg
    21241 tactggagca tgatgtcgta gccgatatag tccgtcagct ggtaaaccac gctgctggat
    21301 acaccgagca tctgcgggtc aataagatat ttaaagacgg ggacggtggt gctggaaatg
    21361 aagccttttt ccctgtcatc cagtggagta tcactcaccg ctttattctg aatactggtg
    21421 agcagttttg taatctgtga tttcagggct ttatcccggg cgatggtcac atttgcatcc
    21481 gccaccacct tcagacactt gtccgaatcg ttgcagtgat aaacttttgc cgttcccccc
    21541 tccataatcg cccggaggat atcgcggtca gtggtacgcg ccggcagggg ggtaatctcg
    21601 ctgtcttccc caaaaatcag cgtaccggtg agcgtcataa taaattcttt cagttctttg
    21661 ttaccatcaa gcatcctgtt tttggacagc gagtcccaga tgatattgat gttcttcagc
    21721 acccgttcct tatctttgtc actggccttg tcacgaacag acgatgactg tcctccgacc
    21781 gtgcagccct gacgggacgc ggcccagtca gcaaaaatat tgctctcacc ggcaatgtcc
    21841 tgacagacct tctgctgtga cacctgagtc tgaggaaata atcctccgac aatcccctgc
    21901 gccacctgac aggagctgag gttcatactg ttaatgtcgc ttgccatttt ctgcaggaag
    21961 tcttttgcgg ttttgatttc cggcaccgtt gcctgcaggg caaggtcaaa aaagtaaccg
    22021 gcagcgttgc tcataatctg cttaacaaaa cgctgcaact gatcgccatt aataaaactg
    22081 aacgagccga gataggcgtc gatgccgcca catccggcat tgatatccgg cagcgtcatg
    22141 gaaatcagct ggatattttt gacctgggtg cgggcataca gggagccacc ataggcatac
    22201 ccggcggcct gcccctgcca gacgcccggt tgtgtggtat tggaagcaaa tcccagctta
    22261 ttaaagaact gattcatgtc gctgttcaca tccgctgacg ctgcgggtgt gaccgtcagt
    22321 aaagcggcac aaagaaccag aagaggttta atgcgaagca ttattgccac cctcccttaa
    22381 tttcttctcc cggcgcatac ggcgtttcag ccggaatatt ctgatccccc cgtttattaa
    22441 ttcatttaaa gggttcctga cgtatacgat aagaaatcca cctgcaaacg cccataacgt
    22501 atacggaatg acatacatca ggatgttcca catataacga aaattattta attccgggtc
    22561 agctttccac gaatcaatac aggcatcaaa aatactatgc ataatcaccc ataaaaaagt
    22621 tccggcagca acgaacacaa aaaacagatc aaaatatata tcagaaaccc tgcgttcata
    22681 atcataatcc cgtttagttt ttctgttttt tagttccgca tcatttcttt tttctttcat
    22741 tataagtttc ctctgatatt tccgatgatt cattcagacc gttcatgcgg ctgtaatata
    22801 tttcagacac cgaaatccat cccacaattg cccccacaaa aaacacaaca gaaacagaaa
    22861 aggtaagaca cagcgaaacg acaatttcca cagggctggc gggataaaaa agcccctgac
    22921 ggataaaggg gatattcagg ctgacgatta ttcccgtcgc gatgccggat attccggcaa
    22981 tgagtaacac cacctgctta tttcgtacct ttttcctctc cgtacatctg cagaacggta
    23041 tccatccgcg ccataaaact ggcggcatcc gttgccccct gcaaaagcgg taatgcctca
    23101 agtgtgttga cgttgaccag gaaggtagtc ggtgtggcca ccgggatatt cgggaagaag
    23161 gtctgcatca cgtccggtgg caccggtaat gcttccggaa aggcggtatc cccctgacca
    23221 tccagggtgt agggaaaaac ggaaaagccg tactgctgtg ccagctgttt cagtaccggg
    23281 tcaaactggt gacagtaagg gcaatgcccc tgcataaaca gcaccacctt ccagtcggcc
    23341 aggttaacct gcctgccgtt gctgaggcgg aaccagcggg gagccggctt ttctgttctg
    23401 gctgatgtgt ctgctgccgg ttgtgcaggc tgcacggcca taccctgcgg attccagagt
    23461 cgttcaattt catcccgggt ggaggcctgc acacctgtgg ccatcatcaa caaaatgacc
    23521 ggcagtaatt tagtgagaga catgtccgcc ctcctgctgc ccttccatca tggcctgttg
    23581 cctggcttcc agtgcctcac gctcttccag gctgacacgt gcaatccatg catccagcac
    23641 ctggtatgcg ccgaacagca caaggatggc cgcaatcaac tccgccagaa aaaaagccat
    23701 ccagggtttg ctgtaaacaa gacgggcgac gatatgaaac cagacaccca gggaaaatac
    23761 ccacattccc gtaatatcaa gccggggtaa agagaatctg tatttactta tcatgttaaa
    23821 ctccggttat tatttacatg agagtattca tgtctgtaat attcgcgttt gtgtgatatt
    23881 aatcagatca acctcattat tttgtatggt ttttattacc gttttatatg acttagcctg
    23941 tcataagccc ccttataaaa aaccacatca ctccggacca tgctgatgac aacataatac
    24001 aaacaaacat ccttctgaaa ctgtcaaact tcgtgataac cggtatggta ctgactgcca
    24061 ttgaaacaac cggtacggca cactgattca taaccgcacc acaaagaaac aagaaattat
    24121 ctggcagcat acatacactt atgacattta ctacaaagaa gacaaacgag ctcagcagat
    24181 aaactccttt attatttggt cctgtaaaga aataaccaac aacccccacg ataaacaggc
    24241 tggcacctgt aaaaaaacac aaatcccctg tatgcatata taacctttgg ttaaaaattc
    24301 ggtttaaaat cttcagaaac gttcaggaac tgttttgcca ggtcgtcctg tgaaataaag
    24361 ccgtatgata acgggcgaac actgccctgt ttcgggtcaa ccagcatcat ggccgggaaa
    24421 tatttcacac cgaggcgctg cgcctgcccc tggtcagtcc gggaatccgg caacagcgga
    24481 ttaatcacgc catccacgga aacgggaata acactcagac cataggtatc cctgaagcca
    24541 ttaatgacct gcgccagttg cccgtcgatg gggtcctgcc cccggtaaaa gaacatgatg
    24601 ccgtagtgtt cagccagttt cgcaatggcc tgtcgctgct gcgcctggtc tgccgccagc
    24661 tggttccgta ccgtgccgtt gtaatggctg tactgcaggt tatagtccag ttcgggatgt
    24721 gccagcatgg cctttttagc actcatggtg aaaagcccgg cctgctgagt ccagtaattc
    24781 tgcagccgga aatatttaac aaaattatcc acgccgggat acagaatggc ttcgtacagc
    24841 gcccgctttg ttgccgtctg cagcgcggcc agtttctgca taatatccgg ctcctgacgg
    24901 ggggctgcag gtacaggttt attttctttt tccttcggat ttattttctc gttataccac
    24961 tgccagcctg catctttccc tgacgccgga aaaataaagc agcagagtaa cagtggcagt
    25021 aatgctttat tcatcttcat tcctttcatc attttttctt tcatcatctt tctgcaggcc
    25081 agtcagggca gaaaaacaaa aatacagaat aaacaggagc acaccggagt aaaggataac
    25141 gaaagcagcg gcaagcatcc cgtctgccgt ggtttctttt accgtatccc acgaccagat
    25201 aattaccggg gaaatgacaa tcgctgtaat cgaaagccgg ataagaaaat caacaagacg
    25261 attagaagtg aatataactt tcatcatttc ccccggttat tttccatacc ttactgtcct
    25321 gcctgtttca gctggtcagc aatctgctct ttcactttct gcgtcagaac gccactgtcc
    25381 gggattttct ggttattcat caggtcttcg tagaagttag tgaaatccag tctgtcgaac
    25441 tgaattttct gcagctcatc aaccgtaatc ccccgacagt caggatgctt cgcactgcca
    25501 aacccgatac gcaactgccc gttacgcccc tgctgctgga ctatctgcgc cagcttcgaa
    25561 tcaaactggc agtagctgcg ctttttctgc agacagacac ccagcacttt cttcgaacag
    25621 aactctccga cactgacggt cagtttgttg tcctttgcct ttcccagcgc cttttcttcg
    25681 ctgttgcact tcgccagacc cacatcctga ccccagccac catccttaca gcagttactg
    25741 aagccggctg cggccttacg gcagaatttc gcctcacccg tgaaagccct gacatccaca
    25801 ccattcaggg cggcaacatc cttacctgcg gcggcaagcg cagccagctg tgatactgct
    25861 tcaccaaaat cactgctttt tccgctctgt gccttatcgc attcaccgtc aaggcagaag
    25921 acatcaccac cacagaccat cacttttcct gatgttctgg actcgcagga gtacgtggcg
    25981 tactcatgca gacaggttcc ctcatcggaa taaaacgcgc actgacgtga ggccagcgta
    26041 caggccggat tatcgacata tttctgacag gtgccgttat ctgcggactg agtgacatat
    26101 ttatcccggt atgcccagca tgcctgtgtg atgttccagg gtttaccttc catcactccg
    26161 gttttcgtac cacccggctc tgaacactca gtgcctgtca gcacgccatc cgctttatta
    26221 aacggacaac tctccaccca ctccacccgg ggaacccatt ctttatcacg gaccttcatc
    26281 ctgagtttca gcgtaaaggt ggaagcacca ctgacaagcg attcatagac tttcctgtca
    26341 ccattcccgt gcgggaggca attaccgtta gcagtacagc cactaccggt cagaacctgc
    26401 ccctgtgtca cctgcaaacc tgtcgccccg gacagggggt aagtatccga ctgcccgttt
    26461 gccaggccga cattaaaggt tgtattcatg aaggtataac gggagttcag gaagaaaaaa
    26521 gcggcgtata cagacaatga tgcactttcc actgtaccgg ttaccggagc cgtgacactg
    26581 aacaccagtt tcagcccgtt catactgaac cggaactgtg agtgcggtat cgtcacttca
    26641 cgatattcat ccgtgtattt ccagtccccg gtgatactgg cagtccgtgt gcagtactcc
    26701 tccaccatcg tgtcccgctc acaggtatag tttgtgaatt cactgcggtt tatctcctgc
    26761 gcactgcact gctgtccggt atttcccaca atgctgtcag cccggttcac cacatccctg
    26821 ccggtctgaa tgaacggtgc atccggtgac agaatgtctt tcggcttgtt catgaaggac
    26881 tctgtgatgg tttttccggt ttcaccggtc gcccattccg tggtgccgtc atttttcagg
    26941 ccgccatccc cgccggcagt cacgccaccg taatattttg tctcgtccgg attcgcgtta
    27001 tagctgggga tactctcctg tggcttaaag ccctgaatac tgcctgtacc ctgcccctga
    27061 atctgacggg caaaatcaga accggcccgg taatcactgt tgctgtcagc ctgtgccatg
    27121 ccggcaacca gagccagtat cagaggtaaa atacgtttca tttcccggaa tctcctttcc
    27181 ctgccagtaa atcatgagcc acctgccggc agtcacccgt ggcggccact ttctccagcg
    27241 cctggccgac ccgcaggttt ccccggatga tgtcgtatcc ctgactgcag aacaccacca
    27301 gtgccggtac actgcgaatg ccgtactgtg aaaacagcgt cgggtcgatc tgaacaccat
    27361 cagtcgcgcc gtctttcacc agggacagca cggcttcagc ggtggtcttc aggtcattgt
    27421 tcaccatgcc ccgcagtgtg gcgggtatac cgtagtgccg ggtttcgccc agcatccgtt
    27481 tcagcccctc ttcgggaatg gaaaacgaca caaaatacag ggcaccctgc cggggtttcc
    27541 cgtcctgcga agcctgctgt ttacgaacca gctcatccag aaaatggtta tctgaacgct
    27601 gaagggggtt ttccagcacc tgtttctccg cccaggcttt cagctgatgg tcaggtttct
    27661 ctcgcagttg tctgcttaaa ttttcctgct gcttcaggaa ctggcggttt tcaggtgtgt
    27721 tcacattttc tgaagcatga acagcaagat gcatccctgc tgtcagagca aaaagtgcaa
    27781 atattttccg gttcatgata tttctcactt tctgctattt acaggcatga actcatcaga
    27841 aaaaagataa ttgagaaaaa cagccataaa cgcacatgta aatgtcacct tcagatataa
    27901 tctgacaagt gtcacttgtt ctgatatatc tgccttcttc cattcatgaa taatttcagc
    27961 gagaaaactg tcaggtgcaa aataaaataa tataacgggc accattacaa gaccaaaagg
    28021 aaataatttt aatgttctgc ttatcagcct gagcagcaca gcacttattt tcctgaggtg
    28081 ctgaatatat ttactgtcca ttgttgccat tttcatttcc tcttattcat taaaatgatt
    28141 tacaggaata cacagttacg tttacgccac atcaggtagc cgaagttttt ctttgtgttg
    28201 ggcggatttt ttcccgtctc ccagcgcatc acactgcgcc cgaacggatg gcactgcccg
    28261 ctgtccggat acatattcac catctgataa cgccagcgtt ctttgggcag gattggggac
    28321 ggatattcat tacagacggc gttatttttc ccgatggttt ccataatcat gccctgacgg
    28381 tgtagtttga acgccatgcg ttcactgacc agcagggagg actgcaacgg actggactca
    28441 ttactcaccc agccattgaa cgggtacata cttccctgcg aaccggcaca ccagaacaga
    28501 acatcgagag gcatattaaa ggcgctggca atggcatctg ctgcacaggc cccctgtgct
    28561 atcggattgg caaagatgac agcttccgga ttgagaatgg tggtcaggct gctgtccgtc
    28621 caggtggggt caatttcaga aagataagcg atatccaggt caccaccttc cagacagccc
    28681 agcgatgtga tgatgttcag ccagtacgtc agcgggtatt tgtaccagtg aacgtgatag
    28741 aacgccccgt tcacctgctt ctcatccttt ttcgccgttc cctgtgccgt tttaccaaaa
    28801 gccggcaggc tgaagcccag gttaaccatg catcccggtg accgggtgac gtccgtcagc
    28861 gccatcggct cccagtaacc aatggccagc ccgatacgcc tgaacagcgg cggcggtgcc
    28921 ggacaaatct gaatgggcat cgacgggttc gccgtgtcgg ggaccttacc ctgactgact
    28981 ttgacactgc ccagcgagag cgggaaaata cagctccagc agatatccgt gatcgggttt
    29041 acaaaacgcc cctcacaggc agaatccgca gacgctgcag ggacatgacc ggcgagaagg
    29101 aataacatca gcagccacag gcttcgcttc attttctgcc ctcctctgcc ggaataaatt
    29161 ccaccttcag gaagcgatcg ccgggaacgg cgctcacccg tgccgggacc tgatcaatgc
    29221 cgaggcgctg gcagagcaca ccgttctggt caaagtaaac acggctgtcc agagacttct
    29281 gcatctccgg gatactgccc tgcacgagga taattttgct ctccagtgtg ggcggcgtct
    29341 ggcgtttcat ccaggccacc tgcgccggat catcgccatt gatgaaatac agcgtctggt
    29401 taaaaggcac atactgcagc ggattcatca cttcaccctg acgggcaaac acccgccctt
    29461 cgttatcccg gatatccgca gccagtctga cggacgggtc aaacaaccgg ctgccgtatt
    29521 tctccgtgcg tccgataccg ggcacggcag ggggccgcag actgttgcgg atcacccgct
    29581 ccttaaaggc atccattttc cggcccatct caccggactg ctccagtgcc gtcaggcgct
    29641 gcatgatcac cgtcagcatg tccggctcct ttaccggcca cagatcgccc caggtgccaa
    29701 gatcggcggc ggccacactc tgcccccata tcagcagggc aatcagcccc cggcatctca
    29761 tggttccgct ctcattcgcc gggcaatatc ctgctggatt tcccgggtga tatccggagc
    29821 cccctgtacc acggcaggcg acaccagaat gaccgcgtgg tgtttctgct gccacgcctg
    29881 caggctggct tccagtgccg tgttaaaacg cgctgaaagg gcttttgact gtgcttctga
    29941 cagttgtttc tgactggcgc tgtcaaaaaa tgcatccaca gtctgtttca tgttgaaggc
    30001 ggcagtcacc ggtgcgttca gtctgacaat gccgtaactg acggctgcgt tcagtaacac
    30061 cattgccagg catcccggca ccgtccacca ccagtggctg cgtcgggggg ctgtaacatc
    30121 agtcgttttt tgcgttgtgg tcatgcaaca ctcctgtatt tctcatgttc ttccagccag
    30181 gcttccagcg aagccatctc cggccctgac ttcttccacg ccagctgcca caccgcctca
    30241 tgaatactca ggccctccct gcgtttctgc tgcacaaact caaaatccgg gccgtcagag
    30301 ctgtacatgg cgcggcttaa cgggtccaca aacagacggt gccaggagga atggttttcc
    30361 acctgcagca ggaaggaact gaaccactgg tctttggccg caccaaactt gccaatcatg
    30421 tcgcgctgca gtggctggaa ctgatccggg aataactggt tatatttcgc gaactccttc
    30481 gcactctgtt tgaggataat tttgtaggag gagttacccc atgccgcacg ggccgcactg
    30541 gaggccttgt cagagtcaaa gtcgacgatg ttctgtgtga tggtgatata ggcaccggta
    30601 tggcggcgac aggtgcggta acctttttga ataaactcac cgactttacg atttttgaag
    30661 tccagcagac gccagccttc atcaatgacg ttcagtttct tgagagtacg cggcgtgcgg
    30721 tacatcctgt tctcgatgta gataatcagg gagaacatca ccgcaaccag cagtgacgga
    30781 cggtcttcca gtccgcccag ctccagcacc accattttcg catcatcccg cagggacggc
    30841 tcatcggagt taaaatactg gccgtaagtg ccgttggcag tgtactggtc aagcagcaca
    30901 atcatttcgt ccagacggct gcggatggtc ggtgacccgg catactgctc gctgtcactg
    30961 gcgtttttca ggaaatccac cacgtcatca atacgtgccc tgttctcttt ggccagccag
    31021 gaggccctga ccgcctgcag cagcagacct tcatgcactt catccaggtt accgttgggg
    31081 ctggccatca ctgacaactg gtcacggaca cgctccgctg actggtcaat atcggtgatg
    31141 ttcgcaaacg ggttaaaacg cagggtttca ccgtccagat atacgccccc catgttctca
    31201 cacagggact tgtagccatc ccccatgtcg aacaccacgg caaagccccc ggagtccagc
    31261 acgctgcgga taagtggctg tatcagtccg gttttaccgg ccccggaggt gccacagacc
    31321 gccatgttgt agttggtgtt attcatcccc ttaaagaaga tatcgataaa cgccagctgg
    31381 ttacggtagg tgggcgccag cagacctgcc ggtgtcaggg ggttatccgc cactaacggc
    31441 atcaggttgg ccacattaaa gctctctgcg cgctgaacca caccggcctc tttcagctgt
    31501 ttaaacagcc ctttcccggc cataaagggc aggcaggtca ggaaattgcg catgtggtta
    31561 aagcgcggtg aaatcagctc aaaaccgttt ttacggaagc tgttcaggat gtcctgctcc
    31621 acttccagtg ccgtttcatt attgtccttg cagaaggctg tgatgttgag gaagtaggac
    31681 acgacagaag actggccgga gcccagccgc tggcgcagtt ccccccactc cttcgcctct
    31741 ttctccacgg acggaaacca tttggcatag gaggtcttcg acttcttctc caggtccatg
    31801 tacttcaggt tggcttcgct gtgggttttc acctggtctt ccaccaccag agtcagcgtc
    31861 aggatgaacg ggcaggaaat ggacatttcc gggttcagca gattgctgta gttgtcggcc
    31921 atgttccaca ggaaggcgat ttccgggtta cgggccagat ggaaattcag gatgcgggcc
    31981 gtgctgttcc tgccgttctc acgcaggccc agcgtcaggt aatcagcccg gacattcagg
    32041 tcaaaactgt cctccacaca ctgataattc agatcagaat acgggtccag ctgacgtctt
    32101 ttcgggtaca gagagtccgg attatggtta atcatctccc cgacaatctc gatgaaagcc
    32161 tgtgcatcca ctgtctgtgt ggtgattttt gcgccgtgaa aagaggcacg gatgattttc
    32221 accagatttt ccatttccag aatgtcagcc cggctttttt tcttcgaggg agaacagtac
    32281 gagataaaca cccggtaatg gcgcagggtc aggggcagat tcatcccctc cggcagcggg
    32341 aactgtgtcg ctgccgcttt catataatag gcacgggtaa tggcgttaaa ccgttcagcc
    32401 tgttcacctg accaggagaa ctcacgcagc ccgtattcaa tcctgtcacc aaccagctga
    32461 ctggacatca gatggataca caacggaata ccgcgcggca gtttggtgcg cagcatgtga
    32521 tcgagtgcct ccacaatgga cttattcgcc ccattgatgg gaatggcttc cagcataaag
    32581 cccatcgtgg tgtcattcat gaacagaccg gattcctggt tgtaatcacg gtacggcagc
    32641 agacggctga actgcgggaa gctcatttcg cccagaactt cattggcctt tgcggattcg
    32701 tcaggcagtt tcagtgctgt gacgagggag ttaaccgcct gagtgacggc ctcaagtggg
    32761 ttattactca tatgagtatt ccttagtgcg tttaaaaaag gaaaatatca gtctgtggcc
    32821 gtggatatat cacgcagtgc cattcctgaa accacagcac aaataagaga tggaggcttt
    32881 taacaggaaa ccaggattaa cgttccttta ctgaatgagc atcatttaat gcctgaattt
    32941 gtttttcgcg aatcttattg cgctcatttt ctcttacccg tttcacctct gccatttcgt
    33001 ttctttttct gcattctgat gcgattttat ttgccttacc aagtgttaaa ggttcagact
    33061 gataattaag tgtctcctga aatgcttctg ctgcacaggg agtttcctgt aaaatctctg
    33121 tcagttgttc tggtgttgca ggttgttgcg caaggtaaac gatgtaaaaa ggaatacata
    33181 caatcatagc cataacgcca acaaatataa aagccagaac agattttaaa tatatcgact
    33241 tcatttcatt ttccttcgaa gcagtaagca ctcattgccc accaccattt agtgtttcca
    33301 tcacgttgtt caaaggttca tttgttttaa cttcaataca ttcccctgta tttccaaagc
    33361 ccctgcctcc gtgtgcttcg attgtccgaa gaacctgcac gcatacgtcc gtatttggca
    33421 tgggggtaac attccagctt ccgccaccgt taatattttt tatcagcaca agaaaaataa
    33481 aggtatgcat tctttattac ctccgcttat atattaatca ctgaatatcc tttcagttaa
    33541 cttatgaggt cataaaacac tgcagatttt tcattcggat tttttctctt cagtaaactt
    33601 cctgatggta tcctgttcac gctgacgggt ggcagactcc tgcattgcat ccactttctc
    33661 ctgccagaga cgccggatat cctgttcatc ccgaccagtc agttttttac cggacaacaa
    33721 acggttaagc agttcctgtc tcacatctgg tacgtcttca gtcagacgca taagctgaag
    33781 caggctctga cggctgagga ccatgagtga ttccggcagg agggtgaaga agaaaataaa
    33841 aaagcatacc gcaccacagc aggcaaggac cgcaaacagg attttcagat atataccatc
    33901 aacgccgaac gcagcaataa tacagggaat ccatgcaata cacgcacaat acgccaggat
    33961 gttatttatt tggttatgtc tgcgatatat ctgttgatgt tcatccagaa tagaatctat
    34021 tttttcagct tctgatttat gcatagtgtt tcctctgcgt ttcctgccag agacgccgga
    34081 tatcctgttc atcccgacca gtcagttttt taccggacaa caaacggtta agcagttcct
    34141 gtctcacatc tggtacgtct tcagtcagac gcataagccg gagtaattcc tgacgactga
    34201 gtaccatgcg tgattcctcc ggtacgctgt aataattaaa aatacgactg aataccccac
    34261 aacagcaggc aacggacagg cacagggatt tcagatacag gccagaaaca cagaatgtgg
    34321 caacaccaca aagaatagct ataactaaaa agcccttacc cagtgccata cacaaagaac
    34381 cctgttttct gaatatctgc tcatgctcat ccagaataaa atctattttt tcattttctg
    34441 atttatgcat agtgtttcct ctgcctttcc tgatacgcct gacattcaac gcacaacgtc
    34501 acaccgggaa atattttcct ccgggcttcc ggaataggat ttccgcatgc ttcacagaga
    34561 taaacaggaa taccatgaac atttattttc tggcgtatcc ggtttattcc tgtcatggtc
    34621 agttgttctg tcactgaata tgcttcatcg gcttcatcac tcacggaata ttcctcccga
    34681 catgttctga cacgctaccg cccatccctt ttcacgcaca gggcactcgt tttacaggca
    34741 gattttgaat acagaaagcc cgccggcatt gccggctgat atacaacttc agggccagtt
    34801 aattaatacg tggttttccc cacgcagacg gtttaataac aaaaaacaca ctggatggct
    34861 gatgataaac atcctgatta tcaatataag gcgctatcca taatgcagcc gtctgctcac
    34921 ccgttcttag cggacgggga ggtgttaccg gcgcaactga actgaccgga acaaccgttt
    34981 tcacttcccg ggcagcggta aacagcgggc gtggtgccag cagtttctgc tccggatgtg
    35041 ctgtcaccgc aggcctgctg cgggacggcg ttgtcgccgc aacggcctgc accgacgtgg
    35101 tccggaagtt cccttcagcc aggcgcggca gtgatgccgc aaccggcttt gcttctgagg
    35161 actgctccag ttttttggcc ttctcattag cctgctccat cgtcatacag gtatctgacg
    35221 tggtggcatc acactcaaaa tcagagttcg ttcctgcaca gccggaaagt aataaggttc
    35281 ccagcagagg aataaataaa gaaatctgtt tcattaccct tctccttttt tattactctc
    35341 cacaacagca gaaattaact ctactgtcag atttgttttt cctgcacagg gttgtccgga
    35401 gactactgta tttcccggag aagaaaataa actcatcgac atacccttct tcatcactcc
    35461 gacagcagcc atattaaaat tgtgcggcaa tgattcagaa ctgaatttct tcggcgcagg
    35521 aagtgcgatt cttgtggtgc agttactgag aacagcatgc ataaaatcat ctgatacgtt
    35581 tttatccggc acagcgcggg cagtaataac attaatatta cgcatgttca tttttatctc
    35641 cgttgcttct gtttgttctg tcatcatgga aataaacagg gcatttcctg attttttccg
    35701 gcagggaagc gtcttccgta taaacttcct tcagatgttc atctgcggtt cccacccgga
    35761 cacatttcat ttttccgggc cacatcggat atttcccggt gcctgccgca aacgacatat
    35821 acatcagtgc cagcgttgcc cagaatgcgg tgaccagttg catcagtgac cggtcagtct
    35881 ccctgacata atctgcagca tcagccgcgc ggtaatggca gatatcctgt atttctgatc
    35941 tgcctgaaga atcagaggaa gaaaaccagc attcactgac cgggaactgt ccggcaggtg
    36001 ctgtttgtct tacgaagtta atctccttaa ccagtaattt ccccggcgtg gtgttgtcct
    36061 tccataaaac aaacagagcc atcagggcca ttgtggccag cggccagata accacaaacc
    36121 tgacgaccca cagcagccag cgaacgacaa acgccacacg gaatgtcagt gcatcacccg
    36181 ccatttccgc cctccttgcc ggcatccccg cccagcgggt tcttgtctcc ggacataaac
    36241 tgctgcggat tcagattgcc cagctgtttc agcatctggt cggtgttaaa accgttcagg
    36301 tggctttctg ccgagggagg attcggcaca gggctttccg ggttaccctc ttccgctgta
    36361 ctctgtgact gctccagcgc catttcctcc acggttttca gctggaagcc gtcctggaac
    36421 accacggtca cttcgttgcc cgcaccaatc ggtatcaccg ggtgatactg ttcggcacgt
    36481 ttgatgtagt agtcactgag cgtctgtgcg gctttcgatg cgccgccacc gatacccatt
    36541 ttcaggacat caccagcccc gtaagcggct gtggcaccca gcccgacagc cggttgggag
    36601 gcgcgctcca ttccctgacc gataccgtca acaaatcccg cgccccatgc ccagccgagg
    36661 attttgccgt tacgcatcac cacttcgccc ttgataccgt ttttaccccg gaagctgaca
    36721 tgccccttaa tcggcatatc aatagttttg ccgtccttca ggcaactgat attgcgggtg
    36781 cgtacaatgg cacgttcact ggacacatcc ccccaggctt ccagccccac aaaacatccc
    36841 gttgcgtcat acgtcttgct gttcggcatt tccaccaggc cggtgatacg caactgcatc
    36901 ggcaccgtgg attcattacc ggtgactgag gcattggcat ccgccccttc aatcagcatg
    36961 gctttcgcaa aacttcctga cggaatgtac ggcagcgatg gtccctgttt tccctcatta
    37021 cgggtaaaca ccttacgctg tatccggtta ggcaccggca cagactggta cgtcacctgt
    37081 ggtggtggcg tgacaccatt cccgggataa aacgccgtcg gcggtggaac agcaacgctg
    37141 ccctgcggcg ggaaggatac gggggtgttt cctggctgtg gttcgccttc cggcccgggc
    37201 ggtgaggcag gcatctgagg tacaggctca ccggtcgccg tgacgggatt agcacccagg
    37261 gcttttacct gctctgccag ggcggcattg tcctgtccca gcttttcaat acgacgctgg
    37321 tcatcaccgc gctgtttgtt caggacatcc agttcacgac ggatttcctc atactgcttc
    37381 tgcatctgcg ctgccgtcac ctgcatctct gtggtggcat gctgacgcac cttgtcatca
    37441 aaggtcgtat ccaccacacc tgtcatatcc ggcacaggct cctgttcagc caccgcctca
    37501 ccgttaccgg acatgtccac atcagacaga tacagtaccc caccaatcgc ggaggctgca
    37561 ccgacaacca caatccccag ccacaggtac tgcttgcgtt tcacaatggt attgatactg
    37621 gccatcagtt gccctccccg tcacggatga catacacatc catgcgcgca cctgccagta
    37681 actggctggc aggctggctg aacatcacgg cacgggttcc cggctgccag aaatcacttt
    37741 cccggatatt cagggcagac agcgttttat tttccacggc aaagcggacc atcttcaggt
    37801 gattccccgt ccatacggaa tctgccactg aagacagccc cgccggggcc tgcagggttt
    37861 cctttgtcac aggaacctga taccagcctg caggtaattt tccgccacgg acagcctggc
    37921 tgatggtcac aagcagggat tcgtaaggcg tggacgtttc ccacgctccg gcttcttctc
    37981 cggttccgcg caggtcactg accagctgaa tggtacgccc cgcgccttca cggggaacag
    38041 cctgaatgga aaggttcaga ccacgctctg tttccagaat gaacgtaaag ggctttttgt
    38101 tgacggtggc caccaccaca ccgccggagg cggtctgctc attattggtc agggcaccat
    38161 ccagactgtt cacggcgata atccggtcac cgggaacggt aaacagattc gggctggtat
    38221 tgctgatggc aatactggcc tgaccaccgt tcaccattgg caccacagcg ggggccagcg
    38281 taccgtttgc ggccatcacg ctgcaggaaa aaaacaggcc gccgaatatt attgccgtat
    38341 tattttttct catcgtctgt ttccccgaaa ttatccagcc aggtcacccc gttttcccgc
    38401 ttcagaataa ggatgtaatg tttgatatca gtaaaaggtt cggagttacc aatccatgtt
    38461 ttccgaattc cacggatttc cacacgacca tactgaggcc agacacggac actggtctgg
    38521 aaaaaagcag aattcacgtt atctttttta atgagtttcg cttcttcagc cagaataact
    38581 ttcatctggt tctgtgcacc cgggcggata tattgcagaa gcgcctgatg tgaagcatcg
    38641 acgatttccg gtgagacatt cagacggagg gcaataaatg acagcgccat ctgctgcaaa
    38701 taagaggcat cggcactgtt ctgtgacacg gcaaagggca cattaaatcc cattggggtc
    38761 acagcagtgc gttgctcatt ctgaagacgg tagttattca ccccctgaat gaggttaaca
    38821 gagaggctga gaacaataag cactgacata aatataaagg cgatggccat tacacgactg
    38881 gtacttaaac gggcaccgtg ttccatataa caatcctggt atcagttcta tttaatccac
    38941 tgccggaaac acgaatcggg aacattatga aaaataccgc gcagcagggc tgttggcata
    39001 taccagtaaa tcaggtcacg taaccaggaa ctgccccgcc cttttttcag ttttttaatc
    39061 ccgaaataaa ccagaaccgc tgcaccaata ccgaacagat atttcgatgt tatgataccc
    39121 cagccaatac agattgctgc ggggatcagt tcatccagtg gcaggccaaa ccagcggctc
    39181 tggttggtca gtgtttccgg gaaacgatat ttcttaagtt tattctcgtc tcccgacatc
    39241 gttttatttc ctgtcagaat ccggcaacag acatacctac tgcaataaat acagagagta
    39301 tggcaaagcc gaccaggaac ttgacgtttt tggtcatcat gtacatgact gcaccgacca
    39361 gaacttcagc cagaacaacc catttaacga tactggagtc cttaccgaag gtcgccttaa
    39421 ccgtttcctg acctttagcc atcaggtccg tgcctttacc agcagccata gctaactcag
    39481 ggaacaacat catcagaaca gcaaccggga tcactgcacg aaccaggcga agcatattca
    39541 gacgagtgaa tttagaaaaa aacgatttct tttcaacggg cgcagaagca ccctgaacac
    39601 ttaaaacagt attcatatga ttcctctgat taatattgac agatgaaaac taaagttctt
    39661 taaatgttga ctcgttctct tcgatcacct ccctgaatat ttcttcatta tagaaatcag
    39721 gaaaccgttt cagatgatct ctgagacgga tctgaacctc tatagtttta cttctaccgc
    39781 tacgatcttt ggcccgaata agtcggttat ttgtatcttc atcgagatac actgaaactg
    39841 ttctgcttat cccgcctctc gcgttacgcc tcctcactca attcacctct gtttcccgac
    39901 taataaaatc atgattgcac agaaaccgac gaaaaaaaac acgccaaaat tggcgtgcct
    39961 tatttaacat ttacttaaca ccataaaatt cacgaattat acttaatagc gaatctattt
    40021 ctcctttata gatacatgac actctgaata aatcaaagtg ttcaaaaaat ccttttattt
    40081 tccgtttaac tggtttaatt cgatcctcag atgctctgtc tgtaataccc aactctctgg
    40141 caattgttgc gtgaccaaca cccagcatat aaaaagagaa aacaaccaat gtccctggag
    40201 aaagatcatt ccatatcgtt aatttatcag aagtatttgt attacttcca ctatgattta
    40261 acgcaaaaaa tggataatca ggaaacggat taatttgcca taaaacaaca gattcgtcat
    40321 tataaaaaga aacattctcc atccttatct gatagatttc gccatgaaaa ttaaaacgac
    40381 gacaaaatgc agagccatgt ccaagagcct ggcattccag ttcaagtgat gaaagaaaaa
    40441 tttcaacctg cagacgtata taactctctc cggataaggg tttatcttct ctggaaaaga
    40501 gttcaataaa agccccattg gcataaagga ttttccggtt gttgttacgg acacaaaccg
    40561 gaacgggaga atttctgtag atattctgaa cgttaaggat gtatttattt acagattgac
    40621 tgttaagtgg cctttctcta cgatccagcg cacacatagg aacctccgtt aaggattcta
    40681 tggacagtcg atgcagggag gtctgaactc cctgcatcgg cgattttcag tttcaatttt
    40741 taacgtggca ttaaccacgt aattaatata acctttatgc ggttaattgt catcaaattg
    40801 aaccagatca aaatcctgtt atttgaagca aatcaacttt taattccttt ttattcctca
    40861 tcattttctg gaaagaacct ctccatctca gatgacacct tatccctgat atcttcgacc
    40921 atattcgcat attcaaactt tggattcccg gaaacatgag ggcttagtga ctcaatacct
    40981 aaaatcttag caactgttga ctgtgttttt accgcacatt caagaagtac cttattaaac
    41041 tcagcctgat tgaacgctga ctctttacgc tccatctgag cctcatgtac acgaagccct
    41101 aattcgagaa gcatagtgga tatgcttgag aaacttacat ctgtactttt agctccttct
    41161 gcacgtcttc tttccacaat tttatttatt ttatagacaa tttcgtcact gacataagcc
    41221 tgcactttcg ccatatgtat acctctttat tttcttatcg catcaaattt atttcacata
    41281 taaaaaaatg acaattcatc gatgaatcga atcgtgacgc aagttacgaa aattacgatt
    41341 catgcagcaa atcacatcac gaattgaatc tagagtcaat tcgctttaac tcgttgtttt
    41401 attattactg attcatctat gagttgcgtt aaattcatag gtgaatcata tgcgattcac
    41461 ctatgaatcc tttttataat gtaaaataaa ttaaaataca ttatttaaaa cataagttaa
    41521 tgattcaaat agcaaatcag caaaaacttg tttttgcgta gtgtgtggtg cttttggtgg
    41581 tgagaaccac caacctgttg agcctttttg tggagtgggt taaattattt acggataaag
    41641 tcaccagagg tggaaaaatg aaaaaatgga tgttagcaat ctgcctgatg tttataaatg
    41701 ggatctgcga agccgccgat tgctttgatc ttgcaggtcg ggattacaaa atagacccgg
    41761 atttactgag agcgatatca tggaaagaat cccgttaccg ggttaatgcc atcggtatta
    41821 atccggtaac gggatatggc agcggactga tgcaggtaga ttcccagcat tttaacgaac
    41881 tggcccgcta tggaattaag ccggaacatc tgacaacaga tccctgtatg aacatttata
    41941 ccggtgctta ttatctggca atagccttta aaaaatgggg cgtctcctgg gaggccgttg
    42001 gtgcatacaa tgccggattc aggaagaccg aacgccagaa ccagagacgt cttgcctacg
    42061 catcagaggt ttaccggatt tatacctgga taaagagcag taaaggcatc cgggttccga
    42121 ccacgaagaa atcactttcc caaattaaca gtgtgcagaa aaattaatat tgtatacatt
    42181 gtcaaatacc ggttatgccg cgacagcggc atggaaggcc acacacaaaa atatcatatg
    42241 agtgataatg ctgactggcc gaagccccgc aaagcgggat cccatcttat atatatccgg
    42301 taactgcgca gcctcctgcc agaccatttt tcgcgggcac aatatttaca gactaccgaa
    42361 aagccagttg atgaaattga ggcatatcag acgacacagc aatgccgcag gatgataata
    42421 taagtaaagc cccgaaaaat ttttcggggc tttacttata ttatcatgac ttccactccc
    42481 tgaactgttc ggcaatcatc cataatgctt tgttgattcg gatatcaccg tcaatgcctg
    42541 taatggctct tgttctggta tttttaccgg aggcacttcg ccccgataat ccacctttta
    42601 tcatattctc ctgaacccgc tgccaggttg tccagagatc gttctgttta tcctcccaac
    42661 ggcggggagt aattatttgt tctggcgtca ctggcgtttt attttcatct tcgtacctgg
    42721 ccatcaatgc agctctgcca aataaatgtt gttcgtcact gttaagatgt atctctttca
    42781 ttgtctccat attttcagtg actttatcaa agacaccgag cacttcatac gctccctcaa
    42841 taacctgccc gacaatatca cctttatgtg gaacgcggat ttcgccaaaa ttattcccac
    42901 acaccaggcc atttgtgcag acaaaacgaa aaattcctgg gatcatctga taactggatg
    42961 aaccatcatg tgaattaagc aggataattt caggaacttc tttcccgtta atatgccctt
    43021 ccctgcgaag acgtaacata tgtttactgt attcgcggcg tcccaaatca cgaacccgac
    43081 tctgacaggc aaagaatggc tggaaacctt catcacgtaa cttattgatg atattgatgg
    43141 ttgggatata tgtataacgc tcactccggg attcatgttt atcaccagaa aagacagatg
    43201 gaacaaactg cattaattcg tcatccgtta acgggcgctc acggcgaata gaattatatc
    43261 gaccaaaacg ggaagctaat cgcataacag taactccttt atgaaggcat aaaattaata
    43321 aaagtttttt cgcttaatat catcccccct cggttatcgt taacaaaaag acaacaattc
    43381 cgaggggaag gaccatcaaa tcaggtcagt tcgggactga ccaacctgcc gagtgaaaag
    43441 cagtaacagc gcgatcaact catcagcgtt ttttctgaca agaggagtat gattctccag
    43501 gctatccagt aagtcatgca gctcattatt gacctggtgg agcaccttaa agagtgcacc
    43561 agaatcccgg cagctctgat atgcaagatt tgcctgaaca gcacgattct gcatgatccc
    43621 gatttgtgcg attgcgtctt tctgctgatc gcctgttggc tgataagtgg tgttaaaaga
    43681 aaatgcgctc ataattcctc ctgtatctga tgttttcagc ccccatcatc tgttgagttt
    43741 cgggagccgc ggttttgacg ttttgcgggg acctgccttt tgttccctcc ggtattcacc
    43801 atcacctgtt tgagaacctt cgccgcaagg ggcatcactg ccacggcctg acgcactgac
    43861 tgtcctgggc agaacgcaaa attttttacc gggttttggg taaaaaattt tgtgggctgt
    43921 tcatttaccc tggacagtca gtgcggcagg ttgtggctgt atcagccccc ggcgacaggg
    43981 gcttaaacag gtgaatggca ccggacacaa caacgggcag ggacccgccg caacgacgtg
    44041 aagcgcggtt tgcgaaacgg cgttgcgcct tacatcccgg cggccttttg ccgggatgcc
    44101 aggagcgcag gccacggcgc gacagaggcg gcgcgaccgg aacggtcgct gcaagccggg
    44161 ggttttgcgt cagggatgga agcccgccag ggcggagaca gcttcgctgg ctccgttcac
    44221 gacagcccgc cccgccaggg ggacgcccgg aaaaggcata agcaggagaa caacaaaaca
    44281 gacaataatc agggcagcag taatcagagt cagagcagta atcagagtca gagcaggagg
    44341 aaaaagagaa taatcaaagg gaaggtgatg tgatttctgg taagtcataa acagagaagg
    44401 cgggggaaaa gaacgggcgc cgcaggcgca cgttcatccc cgttcccgga gggaaacggc
    44461 cttcctggca gtctggttgt tcatgcccgt acaatgccag caaggagaaa ggggccaccg
    44521 gcgaaccagc agccccttta taaaggcgct tcagtgcagt cagaccagca tcagccctga
    44581 aaaggcgggc ctgcgcccgc ctccaggttg ctacttaccg gattcgtaag ccatgaaagc
    44641 cgccacctcc ctgtgtccgt ctctgtaacg aatctcgcac agcgattttc gtgtcagata
    44701 agtgaatatc aacagtgtga gacacacgat caacacacac cagacaaggg aacttcgtgg
    44761 tagtttcatg gccttcttct ccttgcgcaa agcgcggtaa gaggctatcc tgatgtggac
    44821 tagacatagg gatgcctcgt ggtggttaat gaaaattaac ttactacggg gctatcttct
    44881 ttctgccaca caacacggta acaaaccacc ttcacgtcat gaggcagaaa gcctcaagcg
    44941 ccgggcacat catagcccat atacctgcac gctgaccaca ctcacttttc ctgaaaataa
    45001 cccgctcatt caggctgttt acgggtaatc tgtgcgattg ttgccgcatc acgccgcctc
    45061 ccggagtttg ttcggcacca tccaccgctc catctcacgg acagcgtgac cggcctctct
    45121 tgctgcaaga ctggcttccg caataacctg ccagaacggc atatcctgcc actgccagcg
    45181 ttccattccg gccagtgacg gaaaacgctc accccactgc cagaacaggg tttccaggtc
    45241 atgctctgcc acccggtcac tccagcgggc atgccagctt ccgactgttt ccggcgaatg
    45301 gaatgccagt tcgatttcag cctgagcgac cagcgcctgg tacgcacggg ctttctgctc
    45361 tgcctccctc gcctgctggc ggctgtactt ttgcatgacc agtgcggatt tgtggcgact
    45421 gcgctccctg acacggaacc gcaccgacgg gaacagccgc tctgctgcat cgccgggaag
    45481 gggaagaagg cagacctcgc cctgagtttc aatcagcaca tcgacggcat acagccactg
    45541 agaacggttg ccgcgcagtt cttctgcggt cagggacggc atgaaaaatc gcaggtcaga
    45601 cagcgatatc ctgcggcttc cgttcagaca gcggaaaaac gcccccgcat acggatattc
    45661 cgccagcgtg tttccctggt ggcggcgttt ttctgtctcc gccaccgccc gccatgcggc
    45721 ctgttgctct gcgctgaggg gaaccagtgc ctgtgaacgt gcgctcatgc ctgacctcct
    45781 tccgccatca ggatatggat gatgtcactg acgctgtacc cctgcgcgtc cagtgacgcc
    45841 gccagtgcca gtgaatggct gacggcttcc ggtgcaaacc gccgctgtac ctgaaccacc
    45901 gcaaacggct caccaccggc attcaccaga accagcaccc agacctgaga gacatcgccc
    45961 ggtgaacata acgccaggtg aaaacgttcg tgggcaggcg tgaaacggac ctggacgggg
    46021 aaaaagccgc cgaactcgct gccatactcg ccgttcatcg tccagttatc cggcgcatcc
    46081 agctcccgca tcacggcatg tgtgagttca cgacgttcat cacttccggc agcccggata
    46141 tcttcatatt cctgtgcgct catggtctgt aatacattca gggtcagttc agttttcatc
    46201 acggtatctc ccttgctgcc gccccgtctc cggcagggcg gcgtgataaa tggtttatga
    46261 ctggcggtca ggcggcttct gcctggtcgt cacggtactc cgtggtttcc gtctctgcct
    46321 gtggacgtgg tgtacacatc cagccaggca cccagcggtt gtctttcatg tggaactctg
    46381 catgttcagc ggcatcgcct ttcttcattt tctccgcgtc ccgcgcagca ccggacagtc
    46441 cggcatcatt cagggcatcg ataatctgcg gcttttgcag gtgtccgaag aagtttgctt
    46501 tcgtcggctg ccaccagtcg cgcatgtgga atccgatagc gctctccagc gagtccagcg
    46561 gactgcgtga cgtgtgacca tgctcgcggg tctgaacgcc gtggatgctg cacgcggtgc
    46621 agaaactgag cagggataac agtacctcct ggctgagtga caggaacgtc gtcatatccc
    46681 gtgaccatcc ctccggtaac agggctgcaa gacgggcttt ttctgccatc agcgccagga
    46741 atgcggcacc gtccttcccc gatggcgcat cgctggtcag cgaataatgt ttacattcca
    46801 ggctgatttg tgctggttta ctgtacgctc cgctgccaaa cacattcagg cagagcgctg
    46861 atgaatcccc taatgatttt ggtaaaaatc attaggggat tcatcagggc agagcgtcca
    46921 tgccagcagt gccagagatt tgtccggttg ctgcatcagt gctgcctgga ctgccagcgt
    46981 gcgttcggaa gacattttcg tcagcagtgg cagactgatt tcctccacgg gcgctttctc
    47041 ctgcacctgt tccgtgcggt cagcgtcgtc agccgcatca tcctcgctgc gcaactgcac
    47101 accacgctgg acacacacgt tgccataacg ccaggacacc accacaccgc ttccggcacg
    47161 catctccggc gtccacgccc tgaccttcgc catgcagcgc atcagcttca tttctgcttc
    47221 cagcaggtca gattcctcac actggttttc cagcgcgtcg taacgcgtca tcagttcgtt
    47281 cagacgttct tcttccgcct ccgtcagcac cgcttccggc tccggcagac agcggtatgt
    47341 tccggcatcc tcacggcact caccgacagg ctccatgcgt ccggcgcacc atccccagcc
    47401 ttcggcttcc cgaaggtgtt cagcaacagc ctggagtttt tccagcaggg cggcatcgag
    47461 cgccacgcag tccacatagc caccctcgtc gtcgctgaac aggtcagtgc gcagttcatc
    47521 tggcgagaag gcatcagccc ccacgaagcg gaatttactg ttccctgcca ccgccacttc
    47581 actttcggtc accagacgac gaatggtctg tacttccggt ttaccgcccc atcccgactg
    47641 acaggcggct tcaaacacct gcacctgacg cgcggtgtcg ttctccagcg ccagcgcctg
    47701 acagtgctcg gtggtgatga ggtcttctgc cagcgcatcg aggatgacag gcgcaaggtc
    47761 agccagtttc agcattcgct gaacgtggcg gggtgaatag cccagcaaat caccgatttg
    47821 tgcaggtgtt ttgccttcct gcgccattgc acagaatccg gcaatctgtt cggctgggtg
    47881 catatcccga cgatgaccgt tctcggtcat cgatgcggcc gtcgccagtt cctgcgaaat
    47941 aattttcacg cggacaggcc agtcagccgg aatgatgcca cgctctgcca gcatgttgag
    48001 tgctgccagt cggcgaccac ctgcggcgac accgtggcgg tcacctggca gggcatgaac
    48061 aaccagattc tgcagcaggc cgaccccctt aatggaatca gccagttcgc tgacggactc
    48121 cgcagaatac ggcaccgtgc gcacattcag tggtgactta atcagcgagg ccagcggcac
    48181 gctcacttcc tccgtctgcg ccagcagggc agacaggaca gtttcctgcg tttttgcagg
    48241 tttacggctt gctttacggg tagattttgg ttcagacttt gttactgaca tgactatccc
    48301 cttattgtgt agttaatgtc tgccggacgt gatccccgtc cggcacctct cttacttaag
    48361 gatcccgaaa tcacggatga catggttcat ccagtacccg gcaccgtcct caaaattcca
    48421 ggtacgccag accattgttc cgttattacg gacaaccagg cgaaactgtt cgccgtggtc
    48481 atcctcgata aagacgttcc ggtactgtgc ggcaacggct tcggcctgtt tgcgggtaaa
    48541 aggaccgtca ggcagtccct gaagtattct gaaatattcg ctcatcctca tatctccggt
    48601 gatgccccgc gcaggacggg gcggttatca cagtcaggcc agtcagaacg ggatatcgtc
    48661 tgaaaacccg taatcctccc cctccggtgg ttgcggctga ggctccggct gcgcagcctt
    48721 acgtccacgg cctttcgttt tcgcgctccc ttttttcgtc gcgtcagcac tctgtgactg
    48781 accattctgc tgaggctgcg gttgcacctg agcgttctgc tgtggtgcac tccccagcat
    48841 ctgcatggtg cccgtggttt taacaaggat ttcggtgacg taacgggtaa taccgttatc
    48901 ttcccagcta cgggtgcgaa gctggccttc aatgtagacc tgcgccccct tacgcagata
    48961 ttcacctgcc acttccgcga gcttgccgaa cagcaccacg cggtgccatt ccgtctgctc
    49021 cttcatttcc cccgtctgtt tgtcgcgcca ggtttctgac gtggccacct gcaggtttgc
    49081 cactgcgccc ccgttgggga tgtaacggac ttccggatct tttcccagac gaccgacaag
    49141 aatgaccttg ttaatgccac gaactgccat gatgtgtctc cttctgttga tgttttcagc
    49201 ccccatcatc tgttgagttt cgggagccgc ggttttgacg ttttgcgggg acctgccttt
    49261 tgttccctcc ggtattcacc atcacctgtt tgagaacctt cgccgcaagg ggcatcactg
    49321 ccacggcctg acgcacctgt cctgggcaga acgcaaaatt ttttaccggg ttttgggtaa
    49381 aaaattttgt gggctgttca tttaccctgg acagtcagtg cggcaggttg tggctgtcta
    49441 tgcccccggc gacaggggct taaacaggtg aatggcaccg gacagaacaa cgggcaggga
    49501 cccgccgcaa cgacgtgaag cgcggtttgc gaaacggcgt tgctccttac atcccggcgt
    49561 cttttgccgg gatgccggga gcgcaggcca cggcgcgaca agggtggcgc gaccgttccg
    49621 gtcgctgcaa acccgggttt ttgcgtcagg gatggaagcc cgccagggcg gagacagctt
    49681 cgctggctcc gttcacgaca gcccgccccg ccagggggac gcccggaaaa ggcataagca
    49741 ggagaacaac aaaacagaca ataatcaggg cagtagaaaa aagaagaaca gtagcagtag
    49801 cagtagcagt agcagtagca gtagcagtag caggaacaac aacatcatca gtaacagaca
    49861 aatgacaggt ctgaaaagac agaagccccc atcagaacgt ccgcgtaatg aaaggggcgg
    49921 cgcgtgagcg cggccccttt cagcgccacc ggcgcgcggt tttttcccgg cgcggacaga
    49981 cgggcattta tcctcccgcc agccctgtgt catcaggcac gcaaacgaat aatcatcgtc
    50041 gggacatcgg tataagcaaa cgtgccgcgc ggcagctcct cgcggacgtc agaaaacggt
    50101 aacagcttct cctgctggcg tggcccgttg agacagacgg caaccagaac gccccccgga
    50161 cgcaacaggg aaaaagcccg caggatatgc cggatatcct gcccgtgact gaacggcgga
    50221 ttcataatga tccggctgta atactgcacc gactgccatt ccatgaagtc gccacactgt
    50281 accctgacac cgttaaaatt ttcccgcaga taccggacca gcccgctgtt aatctccacc
    50341 gcatcacaca tggcctccgg cgcggtatcc cgaatcgccc gcagtatggc cccggtgccg
    50401 gcagagggtt ccagaatgtg atcacggttg ctgatttccg ccagcgtcac caggcgatca
    50461 cagatcgggg caggactcac aaaaagctga gattcagccc ggatttcagc aaccggcgcg
    50521 gcgttatcca gccttgcgcg catcagccgg atacgttctc ttaactgact gtgcatatca
    50581 ttatctccgt catcccccgg ggatttcccg gggggatata ccttatttct gtgggatttc
    50641 cgtgattttc atgtcggtga tatacacatt caccagacgg aaattattgt ccattgtgcg
    50701 gcggtagcgg tatgccccgt ggtcttctgc ttcttccaca ctgcgcacgg ccttacagtc
    50761 ccggggcagg gcggcccact gcgcctttgt catttcccgg aaaccttccc ccggatagtt
    50821 gactaccggc ggacgcttcg cggcctgact ggcgacggca gcctcttctg ccgatggtgc
    50881 tttgtagtcc gtgatgcgat cgggcgtcac tttcatcgtg ccgctgtacc cgagaaaact
    50941 gtaatttggc gttgtgactg aactcaccgc cccgttgctt ttgttcacgc ggatgatagt
    51001 cagccactcg ccccggctga aaacctgtcc gcccggctca aaatcctgtg tccgggtaac
    51061 cacgccgccg ctttcgtcca gcatggcacg ctcatagatc aggcggttct gatagtggtt
    51121 aacccagcgt tgctgatgct gaatctgccg ttcatgacag cggatcgcaa tttcgcgggc
    51181 ctgctccgtg gtgatgatgt catcatccag ggccgaccat aacgaccggc tcccttcata
    51241 ctggcttttt tctgccgggc gcggatacgt atccagcggg aaacaggcgc tgatatggtc
    51301 atgactgctg atgagttttg ccatattcag atccagcgat tcagcccgcc acatcgtcag
    51361 atatttctgc gactgtgcga tggtcttttc agccttgcgc aaatcagctt cgattttttt
    51421 gatacgacgc cagcgaacgt ccgggcgttc tttatacttc gcgtgaagca gtgccgaccg
    51481 cgcccgttct tcccagtatt ccgcacgttc aaagagcatc acggcacgtt tcatgccgtt
    51541 ttcaatacgc tgcgcatcac gacgggcgcg gcgttcgctg tgatgcccca caagaatggg
    51601 ctgaccgggc gggatcatcg cggccagtct ttccacttca tcaagtgcct gtgcggattc
    51661 actggcccgc tttccgctgt atccggtaaa ccgctccgcc cgtgcttcct gacgttcagc
    51721 gagcgtgctg tcttcatcct caatctctcc ggcaagtgag aggagcacgt cttcccggcc
    51781 cggtgtccag gctggcgcga caaacagcgc ctgtctgggt gcccagcgga aaccggcatc
    51841 atgcactttt ttatacgtct ccgggtcaag acgtgacgcg gcatacaggc gcagtttgtt
    51901 gtcatccggt gagtaggtcg cgcggtacat ctgcacaggg ctggcggctt ccggggcggt
    51961 catggtgatt gcagctaatg tcataaaatg ttctccttcc gtctgttgcc gcccctcgcg
    52021 gggcggctca cctcatcagt taaaagcgcg tttcagttcg agtgtgcgga gcacctgccc
    52081 gaaagccgta tttttcatgg cggggatgtg tacaccacgt ccccggtgaa aactccgggc
    52141 aatgtgtcgc ccgaaggctt tcagcacggg gtcaatttcc ggcgcgggga tgtcttcgcg
    52201 tctgaaacgg ggatgacggc ggggctttac agccgctgcg gaaggtgaaa caatacttac
    52261 cgtcataaat gaaactcctt atgactgttg aatggaaccg cagaaactgc tcgaacagtt
    52321 tttgcggtgt ggattaagca ggtcctcccc ggcctgcttt tttcatatct gcgtctgacg
    52381 ttctccggtg ttttcctcct tctgttcctg cgttctcatt acctgccagc cggatgactg
    52441 acagaacaaa taaatgcgta ttgccggagc ctgaaataaa tcctgatttt taaagaacat
    52501 cacccgttga gtttcgggag ccgcggtttt gacgtttcgc gggaacctgc tgctgtattc
    52561 cctgtggtat gaacttcacc cgtttgagaa ccttcgccgc aaggggcatc actgccacgg
    52621 cctgacgcac ctgtcctggg cagaacgcaa aattttttac cggtttttgg gtaaaaaatt
    52681 ttgtgggctg ttcatttacc ctggacagtc agtgcggcag gttgtggctg tctatgcccc
    52741 cggcgacagg gactcatacg ggtgtgtggc acccggcagg caatgagcag gggcccgccg
    52801 caacgacgga gagcgcgtgc gcgaaacggc gttgcccctt acatcccggc ggccttttgc
    52861 cgggatgccg ggagcgcagg ccacggcgcg acagaggcgg cgcgaccgga acggtcgctg
    52921 caagccaggg gttttcacgc atcggaccgt agcggcagcc cgaaaccgcg ccagcgggtt
    52981 cggcggagac tggcaggtgc gccagcagct gcctgtcgga gtcgggcctg acgaccggat
    53041 gccgccaggc agccgggagc cgaaggtgcg cccggcagac agaacaacac tgcagtagtc
    53101 agaatcagag cagtagataa aggaacaaca acatcaggaa cagttccata acataagaaa
    53161 gaaacactcc tgagtcagac acatcacaga cgggaagggt aacgggcgac gcgccagcgt
    53221 ggcccgcttc ccttccggca ggcgtcagcc tgccaggggt tttcgcgcgg gctgacaggc
    53281 ggcctccacc gcctgcccct gtatcacctg tttatgctgc cctgaaggat gtagtgaccg
    53341 cccaggaaaa ccgcctgctg attcgccaca aaatcgcaga tttcatcaac aatctgcgca
    53401 gtggtgagcg gtttctgccc catttcacga cgccgcgcat taatcacggc tttgtaattg
    53461 cgcagaattt cgcgggtttc tgtgctgata ttcatgacat gtgctccccg tccagcgcct
    53521 gtaacacaac gtccattgct tcgcgttctt ccctgacgaa tgcgtcaccg tcgcccccgt
    53581 tctcgctccc gacggcatac aggcgcttca tttcactgcc tgccagcgcc atcacttttt
    53641 ccagcatcac cgccggaact gtcacctgac ggctggcgcg cttttcttcc atcagctcac
    53701 gaacaacgtc gtgaacaatg ctgacatccg caccatgcgc aaaggcatcg tccagtcgct
    53761 gcatgacggt ttccagctca tcgtctgtca gactccagtc ctgggctatg tgacgcacat
    53821 ccccacgggt cagcaccacg gcaaaaacgc attcactgtc cggcacgtct gtgttcagca
    53881 cttcgcggat aatttcttta acagtacagt acatgttctc gtctccttta caggggcggc
    53941 attgccgccc cgtccgttaa tcagtccagt gccgcgtaaa tcgcgttgca ttcggggtga
    54001 aattcgatgt gacgccacaa ctgcgcatcg cgcatccggt aaagctgggt cagtcctgca
    54061 tccccgctgt cgtggtacag ccacaactgg cggttaatca caagggaagt cagaatgatg
    54121 cctgcggcat cagcactcac ggtacggtca aaccagtttg caccgttcac catgtggaaa
    54181 cggtcgccat ccggcatcat gtaaccgcca ccgtccggca ggcggatgaa gtgccagaag
    54241 cctccctgat attccggcat cattttttgc gccagcgcgt acacatacgc ctcggcatac
    54301 agaaaatcgt tgccaaacag atacggtaga aaggagagac ggtcgcactc ttcaacgttc
    54361 agagtgacag gtttcgcata ttgcatgatg ttttcctccg ttgttcacat attccgcgac
    54421 accgggcggt gccgtgcctc cataaaagcc gggcgataag gcggggatgc acagggcgca
    54481 agggaagccg cagcctgacg cacacggctg gcgggagttg gagggtgaga ccgcgaaatc
    54541 tgagcgggtg aacgaccgga aaatcacggc agacgggaag gagggaccgg gtgatcgcag
    54601 cggcgaagcg tacccttgca tccctgccag cccggctacg atggaggcac ggcacagccc
    54661 gcacacgacg ctgaggccac ataactgaaa acccacaagg ccatgccccg catggccggg
    54721 taaagcggag ccggcggcga cttcctttgt gctctgtccg gggaccggca ggaaaaaggc
    54781 ggggatttac cccgccaacg tcatcatttc cggatttttg ccagggcatc cgccatgctg
    54841 cggatttcgg cccgggcttc ccggaccacc tcccgcagaa caccacggtc aaaccagcgg
    54901 cggggcggtt catccggtat acgcagcaca tcgcaggcca gcccactgga caggatatgc
    54961 tcaatacaat cgctgaccag gtacatttcc accgacgaaa aacgtgctcc ccggttcacg
    55021 atggcatcga gcaccacggg caggttttcg tcatcttcat cgagccagca ggggtgatcg
    55081 ctttccttca caaccagacg aaagcggcgc gtctggcgag tcagcaacgt ctgaccggtg
    55141 gtggtcagcg ccgcccggat acgccgcgca tcttctgcca cccggcaact gacactgcgt
    55201 accgcatcgc gcaccgccgc aagtacagcc ctgatttcgt cttcggtcag ccattctccg
    55261 gcatacggga aagacacctg cgttccgtct gccggaagta ccggcagcga ctgccactga
    55321 ctgccggaca gtaccagttc cgccccggtt ccggtcagtt tctgaatggc ggaggccagg
    55381 agcggcggca gtacgccgcc gtgacggttg ataagtaacg tcgccagccg gggcgcatcc
    55441 agcagaacat aaatgctgat ccagcccgga tacttcgggt tacgctggtc aacatccgtc
    55501 tcttccggcc agccctgcgc cagcagcagg ttgcgggcgt gacgacaaat cagtgcgttc
    55561 agcgtgctgt tttcgttcat gattaagcgt tctccctgta tcgccgcccg cagggcaggc
    55621 ggcgataaca tcagtcgttt tcgtgtgtgt tgtttttcag cagggtgcgg gcctgattaa
    55681 ccacctcctg acggcgggtg acgcattccg ggacggcgag gccatgatcc cgggcggccc
    55741 attcacgttt ccacaatgcc tgttcagcgg tctgaatcag ggacgccagc agttcagcag
    55801 ggacggtcac caggcggttt tcgctctcgt tgcggataat ctccatcacc gcagcggaag
    55861 aaataccgga ctcaatccgc tggtcttccg ggatgtcctc caggcgtgcc aggacggtcc
    55921 tgatttcatg accggtcaga gaaatttcca taccgtcagc cagtgcctga atctcttccg
    55981 gggaccagat aaccagcatc agcggcatgt ctcccggata ctttactgcc agctcccggc
    56041 atagcgtttc gcatgttccg tacattctcg tctccttctc aatctttaaa gcacgcttcc
    56101 agttccagaa tttcggtata tgcctccatc aggaggcggt ttgcgcgaaa cagcgtctga
    56161 cgcaccagac gttccggcag ggtgtcggca ttaaagctgt accgctgctc catctcattc
    56221 atggttgtac gcaggtcgca catggcattg gccacctccg ccacttcggc gcgcagtttt
    56281 tcgtgtcctg catagttcat ttacgcagcc tccggcataa accagtcatc attcgcggcc
    56341 ccctgctgca tggcccgttg cacggcggca aggcgttgct gcccggcacg gtgatactgc
    56401 tcaagcagct cgataccgat ataccggcgt ccggactgga gggcggcgac gcaggttgag
    56461 ccgctgcctg caaacgggtc cagcacaatt gcgttcgggt gtgtgaagct ctcaatcagc
    56521 ggttgcaggc tggtaacggg cttttccgtc gggtgatggc gattgcccga atatttccag
    56581 cccagcacgt ccggcagcgg gttttgtggc agacgtggac ggccttttgc caggatgtag
    56641 gcacattcgt ggcgatagcc cacatatgcg gccttcgatg tgtagttttt ggtgaagacc
    56701 aggtgaccaa caacgctgaa tcccgcattt ttccaggcgg ccataaagcg atcgacgcgg
    56761 ttccagccat agaagctcac cattaacgcg tcttttttga gtacgcggta catttcatta
    56821 caggctggtt gcagccattc atcggtttta tcgccggcga tggtgcgccc ggaacggtca
    56881 cggaaaccga cgagatacgg cgggtcggtg aggatgaaat cgacagcatt gcccggaaac
    56941 gtggccataa cacgcacgca gtcacccagg ataaaacggg acataacagc actccttctc
    57001 ttacagaggg caccgggagg gcacccctgt ggtgcccgtt ccccgctgcc tgtgggctgt
    57061 tcaccgcgtc cggtaacggg tcaggccgac cgtggaatac cgcagccgtc gtctttttga
    57121 cggcgaggat atgccgcgaa agcggcttga agggcgttca tcagaaaagg gcgacgggct
    57181 ggcgtacact gtcaccaacg ccagccggga gcggtaaaag cgcccgtccc gtgcccggcc
    57241 ggacgcggga cactgaccac aggcagcgtt cctgccgcgc cgcgtcagcc ggcgcagtaa
    57301 aaagaggagc ccggcgacat cttttgttac ccgccaaaca aaacccaaaa acaacccata
    57361 cccaacccaa taaaacacca aaacaagaca aataatcatt gattgatggt tgaaatgggg
    57421 taaacttgac aaacaaaccc acttaaaacc caaaacatac ccaaacacac accaaaaaaa
    57481 caccataagg agttttataa atgttggtat tcattgatga cggttcaaca aacatcaaac
    57541 tacagtggca ggaaagcgac ggaacaatta aacagcacat tagcccgaac agcttcaaac
    57601 gcgagtgggc agtctctttt ggtgataaaa aggtctttaa ctacacactg aacggcgaac
    57661 agtattcatt tgatccaatc agcccggatg ctgtagtcac aaccaatatc gcatggcaat
    57721 acagcgacgt taatgtcgtt gcagtgcatc acgccttact gaccagtggt ctgccggtaa
    57781 gcgaagtgga tattgtttgc acacttcctc tgacagagta ttacgacaga aataaccaac
    57841 ccaatacgga aaatattgag cgtaagaaag caaacttccg gaaaaaaatt acattaaatg
    57901 gcggggatac attcacaata aaagatgtaa aagtcatgcc tgaatctata ccggcaggtt
    57961 atgaagttct acaagaactg gatgagttag attctttatt aattatagat ctcgggggca
    58021 ccacattaga tatttctcag gtaatgggga aattatcggg gatcagtaaa atatacggag
    58081 actcatctct tggtgtctct ctggttacat ctgcagtaaa agatgccctt tctcttgcga
    58141 gaacaaaagg aagtagctat cttgctgacg atataatcat tcacagaaaa gataataact
    58201 atctgaagca acgaattaat gatgagaaca aaatatcaat agtcaccgaa gcaatgaatg
    58261 aagcacttcg taaacttgag caacgtgtat taaatacgct caatgaattt tctggttata
    58321 ctcatgttat ggttataggc ggtggcgcag aattaatatg cgatgcagta aaaaaacaca
    58381 cacagattcg tgatgaacgt tttttcaaaa ccaataactc tcaatatgat ttagttaacg
    58441 gtatgtatct cataggtaat taatgatgga caagcgcaga accattgcct tcaaactaaa
    58501 tccagatgta aatcaaacag ataaaattgt ttgtgataca ctggacagta tcccgcaagg
    58561 ggaacgaagc cgccttaacc gggccgcact gacggcaggt ctggccttat acagacaaga
    58621 tccccggacc cctttccttt tatgtgagct gctgacgaaa gaaaccacat tttcagatat
    58681 cgtgaatata ttgagatcgc tatttccaaa agagatggcc gattttaatt cttcaatagt
    58741 cactcaatcc tcttcacaac aagagcaaaa aagtgatgaa gagaccaaaa aaaatgcgat
    58801 gaagctaata aattaattca attattattg agttcccttt atccactatc aggctggata
    58861 aagggaactc aatcaagtta ttttcttacc agtcattaca taatcgttat tatgaaataa
    58921 tcgtttgcac tgtctctgtt attcaggcaa tttcaataaa ggcacttgct cacgctctgt
    58981 cattttctga aactcttcat gctgcatttc gatttttctt ctaaaatcat aacttgcata
    59041 cctgttcttt ttatgtatat ccagatctat ttttgtgacg agttcaaacc acaatgatag
    59101 cactccccat gcagcgttat gaaacatttc cgcaacagat ttagagtctg tattaaccgc
    59161 ttcagtcatg tagtcgctaa tacgttctgc agtgattttc gttatttcat cgtaattcat
    59221 tttatcccct acaagactat ttaatggaat gaaaaatatt tggtctgata acaatgctcc
    59281 tgaattaaca cctgcaggct atgttaaagt tgccctcaca cacaatctac ctgttttctc
    59341 gtgtgtgtca caggcgcaat acataaatag tctggccatt aagttcgtaa cggatttcgt
    59401 actcgccagc aaaaatccgt ctgacatccc tggtttcaaa ctgaaacaac tgttcatccc
    59461 atacgtggat gagttaacag aatgaccggc gcctgtgtca gggactacac cgttttggct
    59521 gcagctggtt tactgaccaa cgccagaaaa tcatataact gcgtcaatgt cagcgtatcc
    59581 agcgcgactg tattattttt ctctgcaacc agaatattgc attaggttat taccaggtgt
    59641 tctaatgtac aggtaaagac gttctttata atctgaaatc tctactttta atccctcaga
    59701 ctttgcctgc tgaaagccct cgtcaaggtg cgcttttatt aggttaatgt ctggatgtct
    59761 tgtatcgggg gcaattctgt attggtgtaa cgatggggtg gatgttccct gcaataagac
    59821 ctccagatag agattggcac aaccattccc attatttcta atagtttatt ggtaatcaca
    59881 aactgtatat ttagccatta tattgaatcc tgtgaagtaa acggcgacgg ctcaaaattt
    59941 tatcaaacct aagataattc taccatcaaa gttaataatt tgaatttata tgacggctct
    60001 atgggggggt aaaaatcccc gtgcagagag gcgattttca gagttgaaaa cgtcgtcgtt
    60061 aagaacacca aagtcagtac caccaacccg gaaagtagct tcatgcaccg ggacaacagc
    60121 agttttggtg tccgaaatcg tacaattctg tacatctacg caaggaaatg tacggaagta
    60181 agccaaatgg ttatcggacg gtcggaacat ttcgtacagc ctttacgttc agtgagttag
    60241 ctggcctaga tgtaaccact cgttcaactc aattctctta gcgtgggaaa atttccgttt
    60301 tccaagcggc cccctcaagc ggacccctta tagtacaata attttcgata tccaaactgc
    60361 ccccaattac atctccagtc tctggcagca gagatcatat atttccattg ccgccgtagc
    60421 cacgatattc tggctattca caaaaacaat ttagccccaa agcgcagtaa aaaagtacac
    60481 tatgaaggtg tatgatacct tcatagtgct taagtaatta tgagtaatat aatctgtgta
    60541 aactactaat tgcttcaccg tcagtgatta aatgcgtagc tgttccttgt cttatataat
    60601 acctattttc aaaatgacag acttctccga cactttttat ttcgattttt aaaacatgca
    60661 gtccttcata tgaacataat gaaatatctt ttagtatttg ttgtttcaaa gcctcattaa
    60721 aattaaatga ttctattttt tgttttatga acagaaagta attatctatg ttttttgatt
    60781 gaattacagc ttcatgatca attccattga tataatagcc attgcactca ataggggtaa
    60841 caccatataa agtcttaact ctgtctgccg tagctttggt atcggaaaca ccaactaaaa
    60901 tataaccatt agattctcta ccgatgttat ttatggcgac agccgtttgg actatattaa
    60961 tgaaacattc ttcgtctatt ttattctcgc ctgagagaat aaataatccc tgtttggtaa
    61021 tgactccaac ttattgatag tgttttatgt tcagataatg cccgatgact ttgtcatgca
    61081 gctccaccga ttttgag
//
LOCUS       DAETPZ010000013        60685 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.013, whole genome shotgun sequence.
ACCESSION   DAETPZ010000013 DAETPZ010000000
VERSION     DAETPZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.013"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..320
                     /locus_tag="K0W59_000943"
     CDS             <1..320
                     /locus_tag="K0W59_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3022083.1"
                     /translation="VGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDS
                     NSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQV
                     TYP"
     gene            433..1227
                     /gene="panB"
                     /locus_tag="K0W59_000944"
     CDS             433..1227
                     /gene="panB"
                     /locus_tag="K0W59_000944"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBI3022084.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1239..2090
                     /gene="panC"
                     /locus_tag="K0W59_000945"
     CDS             1239..2090
                     /gene="panC"
                     /locus_tag="K0W59_000945"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBI3022085.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSTLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVGLA"
     gene            complement(2164..3066)
                     /gene="rpnC"
                     /locus_tag="K0W59_000946"
     CDS             complement(2164..3066)
                     /gene="rpnC"
                     /locus_tag="K0W59_000946"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HBI3022086.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIPQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3340..3720
                     /gene="panD"
                     /locus_tag="K0W59_000947"
     CDS             3340..3720
                     /gene="panD"
                     /locus_tag="K0W59_000947"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBI3022087.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3724..4953)
                     /locus_tag="K0W59_000948"
                     /pseudo
     CDS             complement(3724..4953)
                     /locus_tag="K0W59_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(5017..5457)
                     /locus_tag="K0W59_000949"
     CDS             complement(5017..5457)
                     /locus_tag="K0W59_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBI3022088.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5562..6332)
                     /locus_tag="K0W59_000950"
     CDS             complement(5562..6332)
                     /locus_tag="K0W59_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3022089.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6329..7255)
                     /locus_tag="K0W59_000951"
     CDS             complement(6329..7255)
                     /locus_tag="K0W59_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI3022090.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCLNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7364..8026
                     /gene="can"
                     /locus_tag="K0W59_000952"
     CDS             7364..8026
                     /gene="can"
                     /locus_tag="K0W59_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HBI3022091.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8067..8603)
                     /gene="hpt"
                     /locus_tag="K0W59_000953"
     CDS             complement(8067..8603)
                     /gene="hpt"
                     /locus_tag="K0W59_000953"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBI3022092.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8809..11199
                     /gene="gcd"
                     /locus_tag="K0W59_000954"
     CDS             8809..11199
                     /gene="gcd"
                     /locus_tag="K0W59_000954"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HBI3022093.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11246..12795)
                     /gene="cueO"
                     /locus_tag="K0W59_000955"
                     /pseudo
     CDS             complement(11246..12795)
                     /gene="cueO"
                     /locus_tag="K0W59_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
     gene            12961..13308
                     /locus_tag="K0W59_000956"
     CDS             12961..13308
                     /locus_tag="K0W59_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HBI3022094.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13414..14280
                     /gene="speE"
                     /locus_tag="K0W59_000957"
     CDS             13414..14280
                     /gene="speE"
                     /locus_tag="K0W59_000957"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBI3022095.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQQS"
     gene            14296..15090
                     /gene="speD"
                     /locus_tag="K0W59_000958"
     CDS             14296..15090
                     /gene="speD"
                     /locus_tag="K0W59_000958"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBI3022096.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15128..15490)
                     /locus_tag="K0W59_000959"
     CDS             complement(15128..15490)
                     /locus_tag="K0W59_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HBI3022097.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15666..18263)
                     /gene="acnB"
                     /locus_tag="K0W59_000960"
     CDS             complement(15666..18263)
                     /gene="acnB"
                     /locus_tag="K0W59_000960"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HBI3022098.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIINRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18618..20471
                     /locus_tag="K0W59_000961"
     CDS             18618..20471
                     /locus_tag="K0W59_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HBI3022099.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTLPATVSAPPVIPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDSNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRIQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSTVRERLSERGARRLER"
     gene            complement(20542..21984)
                     /gene="lpdA"
                     /locus_tag="K0W59_000962"
     CDS             complement(20542..21984)
                     /gene="lpdA"
                     /locus_tag="K0W59_000962"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBI3022100.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPNPNPNPKAKKK"
     gene            complement(22309..24201)
                     /gene="aceF"
                     /locus_tag="K0W59_000963"
     CDS             complement(22309..24201)
                     /gene="aceF"
                     /locus_tag="K0W59_000963"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBI3022101.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24216..26879)
                     /gene="aceE"
                     /locus_tag="K0W59_000964"
     CDS             complement(24216..26879)
                     /gene="aceE"
                     /locus_tag="K0W59_000964"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HBI3022102.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27040..27804)
                     /gene="pdhR"
                     /locus_tag="K0W59_000965"
     CDS             complement(27040..27804)
                     /gene="pdhR"
                     /locus_tag="K0W59_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HBI3022103.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28345..29718
                     /gene="aroP"
                     /locus_tag="K0W59_000966"
     CDS             28345..29718
                     /gene="aroP"
                     /locus_tag="K0W59_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HBI3022104.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29761..30615)
                     /gene="ampE"
                     /locus_tag="K0W59_000967"
     CDS             complement(29761..30615)
                     /gene="ampE"
                     /locus_tag="K0W59_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HBI3022105.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30612..31163)
                     /gene="ampD"
                     /locus_tag="K0W59_000968"
     CDS             complement(30612..31163)
                     /gene="ampD"
                     /locus_tag="K0W59_000968"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HBI3022106.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31251..32144
                     /gene="nadC"
                     /locus_tag="K0W59_000969"
     CDS             31251..32144
                     /gene="nadC"
                     /locus_tag="K0W59_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBI3022107.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32347..32787
                     /gene="ppdD"
                     /locus_tag="K0W59_000970"
     CDS             32347..32787
                     /gene="ppdD"
                     /locus_tag="K0W59_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HBI3022108.1"
                     /translation="MDKQHGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32797..34182
                     /gene="gspE"
                     /locus_tag="K0W59_000971"
     CDS             32797..34182
                     /gene="gspE"
                     /locus_tag="K0W59_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HBI3022109.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNTVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTTETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDTVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPAIRQLISANTEVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGK"
     gene            34172..35374
                     /gene="hofC"
                     /locus_tag="K0W59_000972"
     CDS             34172..35374
                     /gene="hofC"
                     /locus_tag="K0W59_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HBI3022110.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALHYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35409..36452)
                     /gene="guaC"
                     /locus_tag="K0W59_000973"
     CDS             complement(35409..36452)
                     /gene="guaC"
                     /locus_tag="K0W59_000973"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBI3022111.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36614..36652)
                     /locus_tag="K0W59_000974"
     CDS             complement(36614..36652)
                     /locus_tag="K0W59_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022112.1"
                     /translation="MIIKTLKMSARK"
     gene            36677..37297
                     /gene="coaE"
                     /locus_tag="K0W59_000975"
     CDS             36677..37297
                     /gene="coaE"
                     /locus_tag="K0W59_000975"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBI3022113.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37297..38040
                     /gene="zapD"
                     /locus_tag="K0W59_000976"
     CDS             37297..38040
                     /gene="zapD"
                     /locus_tag="K0W59_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HBI3022114.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38050..38247
                     /gene="yacG"
                     /locus_tag="K0W59_000977"
     CDS             38050..38247
                     /gene="yacG"
                     /locus_tag="K0W59_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HBI3022115.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38347..38736)
                     /gene="mutT"
                     /locus_tag="K0W59_000978"
     CDS             complement(38347..38736)
                     /gene="mutT"
                     /locus_tag="K0W59_000978"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HBI3022116.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38796..41501)
                     /gene="secA"
                     /locus_tag="K0W59_000979"
     CDS             complement(38796..41501)
                     /gene="secA"
                     /locus_tag="K0W59_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBI3022117.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41563..42075)
                     /gene="secM"
                     /locus_tag="K0W59_000980"
     CDS             complement(41563..42075)
                     /gene="secM"
                     /locus_tag="K0W59_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HBI3022118.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42306..43223)
                     /gene="lpxC"
                     /locus_tag="K0W59_000981"
     CDS             complement(42306..43223)
                     /gene="lpxC"
                     /locus_tag="K0W59_000981"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HBI3022119.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVCVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43324..44475)
                     /gene="ftsZ"
                     /locus_tag="K0W59_000982"
     CDS             complement(43324..44475)
                     /gene="ftsZ"
                     /locus_tag="K0W59_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBI3022120.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44536..45798)
                     /gene="ftsA"
                     /locus_tag="K0W59_000983"
     CDS             complement(44536..45798)
                     /gene="ftsA"
                     /locus_tag="K0W59_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBI3022121.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45795..46625)
                     /gene="ftsQ"
                     /locus_tag="K0W59_000984"
     CDS             complement(45795..46625)
                     /gene="ftsQ"
                     /locus_tag="K0W59_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBI3022122.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46627..47547)
                     /gene="ddlB"
                     /locus_tag="K0W59_000985"
     CDS             complement(46627..47547)
                     /gene="ddlB"
                     /locus_tag="K0W59_000985"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBI3022123.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLKASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47540..49015)
                     /gene="murC"
                     /locus_tag="K0W59_000986"
     CDS             complement(47540..49015)
                     /gene="murC"
                     /locus_tag="K0W59_000986"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBI3022124.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49069..50136)
                     /gene="murG"
                     /locus_tag="K0W59_000987"
     CDS             complement(49069..50136)
                     /gene="murG"
                     /locus_tag="K0W59_000987"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBI3022125.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50133..51377)
                     /gene="ftsW"
                     /locus_tag="K0W59_000988"
     CDS             complement(50133..51377)
                     /gene="ftsW"
                     /locus_tag="K0W59_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HBI3022126.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51377..52693)
                     /gene="murD"
                     /locus_tag="K0W59_000989"
     CDS             complement(51377..52693)
                     /gene="murD"
                     /locus_tag="K0W59_000989"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBI3022127.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPSL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52696..53778)
                     /gene="mraY"
                     /locus_tag="K0W59_000990"
     CDS             complement(52696..53778)
                     /gene="mraY"
                     /locus_tag="K0W59_000990"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBI3022128.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53772..55130)
                     /gene="murF"
                     /locus_tag="K0W59_000991"
     CDS             complement(53772..55130)
                     /gene="murF"
                     /locus_tag="K0W59_000991"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBI3022129.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55127..56614)
                     /gene="murE"
                     /locus_tag="K0W59_000992"
     CDS             complement(55127..56614)
                     /gene="murE"
                     /locus_tag="K0W59_000992"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBI3022130.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56601..58367)
                     /gene="ftsI"
                     /locus_tag="K0W59_000993"
     CDS             complement(56601..58367)
                     /gene="ftsI"
                     /locus_tag="K0W59_000993"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HBI3022131.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58383..58748)
                     /gene="ftsL"
                     /locus_tag="K0W59_000994"
     CDS             complement(58383..58748)
                     /gene="ftsL"
                     /locus_tag="K0W59_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBI3022132.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58745..59686)
                     /gene="rsmH"
                     /locus_tag="K0W59_000995"
     CDS             complement(58745..59686)
                     /gene="rsmH"
                     /locus_tag="K0W59_000995"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBI3022133.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59688..60146)
                     /gene="mraZ"
                     /locus_tag="K0W59_000996"
     CDS             complement(59688..60146)
                     /gene="mraZ"
                     /locus_tag="K0W59_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBI3022134.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCAIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14537 a        16811 c        14921 g        14416 t
ORIGIN      
        1 aggttggtac tgaaaacggg caactccttg gtaatactct cacaggtaat gacgcagcaa
       61 aaggagtcgg tgtactcata gaaggtttag caactagtaa aaatcctcta atgacattga
      121 aacctaatga ttcaaattct gtttataaag attacgaccc gagaggcaaa gacgatacaa
      181 caggaggagt ttacccggat caagatacag gtataacata ccctctccat ttccaggcca
      241 cgctacaaca ggatggaact ataccaatag aagctggtga gtttaaagcc accagtactt
      301 tccaggtaac ctacccttaa taagtccacc gcaccgccat catctggcgg tgcggtaatt
      361 gataaatcat tgcccccacc aatgtgactg ataaaatgcc aggcaacacg acatcattta
      421 tcaggatacg ttatgaaacc gaccaccatt gcttcacttc agaaatataa gcaggataaa
      481 aagcgtttcg cgaccatcac cgcttacgac tacagcttcg cgaaactatt tgctgatgaa
      541 gggcttaacg tcatgctggt aggcgactcg ctgggcatga ccgttcaggg ccacgattcc
      601 actctgccag taaccgttgc tgatatcgcc taccacactg ccgccgtacg tcgcggcgca
      661 ccaaactgcc tgctattggc tgacctgccg tttatggcgt atgccacgcc ggaacaagcc
      721 tttgaaaacg ccgcaacggt tatgcgtgcc ggtgccaata tggtcaaaat tgagggcggt
      781 gagtggctgg tcgaaaccgt aaaaatgctg accgaacgtg ccgttcctgt atgtggtcac
      841 ttaggtttaa ccccacagtc agtgaatatt ttcggtggct acaaagtaca ggggcgcggc
      901 aatgaagcga gcgatcgact actcagcgat gcattagcct tagaagccgc gggggcacag
      961 ctgctggtgc tggaatgcgt gccggttgag ctggcaaaac gtattaccga agcactggcg
     1021 atcccggtta ttggtattgg tgcaggcaac gtcactgacg ggcaaatcct cgtgatgcac
     1081 gacgcctttg gtattaccgg cggtcacatt cctaaattcg ctaaaaattt cctcgccgaa
     1141 acgggcgaca tccgcgcggc tgtgcggcag tatatggctg aagtggagtc cggcgtttat
     1201 ccgggcgaag aacacagttt ccattaagga gtcacgttgt gttaattatc gaaaccctgc
     1261 cgctgctgcg tcagcaaatt cgccgcctgc gtatggaagg caagcgcgtg gcgctggtgc
     1321 ctactatggg taatctgcac aatggccata tgaagctggt cgacgaagcc aaagcccgcg
     1381 ccgatgtggt cgtcgtcagt attttcgtta acccgatgca gttcgaccgc ccggaagatc
     1441 tggcacgtta tccacggacc ttgcaggagg actgcgagaa gctaaacaaa cgtaaagtgg
     1501 atttagtttt cgccccttcg gtaaaagaga tctacccgaa cggtactgaa acccacactt
     1561 acgttgacgt tcctggcctt tcaaccatgc tggaaggtgc cagccgtccg ggacattttc
     1621 gcggcgtttc gactattgtc agcacgctgt tcaacctggt ccagccggac atcgcctgct
     1681 tcggtgaaaa agattttcag caactggcgt tgatccgcaa aatggttgcc gatatgggct
     1741 tcgatattga gattgtcggt gtgccaatta tgcgcgccaa agacggtctg gcgctaagtt
     1801 cccgtaacgg ttatctgacg gcggaacaac gcaaaattgc gcctggtctg tacaaagttt
     1861 taagttcgat tgctgacaaa ttgcaggctg gggaacggga tctcgatgaa attattacca
     1921 ttgcggggca agaactgaat gaaaaaggct tccgcgccga tgatattcag attcgcgatg
     1981 ccgacacatt gctggaagtt tctgaaacca gcaaacgggc agtaattctg gtagccgcct
     2041 ggcttggcga tgctcgcctg atcgacaaca aaatggtcgg gctggcgtaa tacttaactg
     2101 gcgctacggc tgatggcgcc agttattaat ttaccccacg tcatctgtca taacacaggt
     2161 aacttaaata aggttaatta ccttatcaat ctcagcaaga ggtaaatttg ccatctctgc
     2221 aacgtcttcc cgagacattc ctttacttag aagacgcagg gcgaattctt gcctttcctc
     2281 ctgtcttccc tgcggaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2341 catcatagac ttccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2401 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2461 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2521 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2581 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gcgccagctc
     2641 cggcgagtaa aacatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     2701 ataaaacagt atcggcacca ccagcggcag cttatcgtgg ccagcctcca gatgccggtg
     2761 catggcagca atagaataac gcatcatgcg aaaggccatt ttcttatccg gcttgctttg
     2821 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     2881 gtccgtgctg tgacctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     2941 gttgagatca caaagttcgc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3001 cgtctccgca tgcattaaaa attgtttaaa taccgcgtca tgcggtgtgg tactcggtgc
     3061 atccatcttc cctgtcttcc gtgtggcctt gcgtgaaggc agtatccagt caaggcgtgc
     3121 tatcgtcagc cacctcttta ttgtttaccg agcagcattc ataaaaatat caattcgtta
     3181 caggcgttac atggcacgct tcggcgcggt acgcaaactg ctgacgcaaa tgccatcaga
     3241 tgagtagaca ctcatcaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcac
     3301 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3361 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3421 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat cgatatctgg
     3481 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3541 attatttctg ttaacggtgc ggcggcccac tgcgccagcg tcggcgatat tgtcatcatc
     3601 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa tgtcgcctat
     3661 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     3721 ttgttatccc tgcggctggt tactcaccag ccgcgacatc ttctccagcg aatccgttct
     3781 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     3841 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     3901 gccgccaaac ggatacgaaa gataccagac atgtggatta aattgcgcca gagcgcggcg
     3961 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4021 ataaccatct acccgatgca aaaaatgggt atgtgactgg aaatcaaaaa catcgcgaat
     4081 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggctccact tctgcgggtg
     4141 acgtttgatg cgtgaggtaa caataaacac cgtcgccttc atgccatatt gtttcaacac
     4201 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4261 cgcagggaga ttgatcgtat tcttcacgta accttccagc tgagccatgc tcagtgtcgc
     4321 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4381 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4441 cggcaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4501 acggatctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4561 caaattgtcc gccagtaccc caaatggcgc actgcccaca ctcggcgcgt tgtagaccgg
     4621 cgtatctttc taggtaatta agttctgatt actcagcggc ttgttaaggt cacccaaacc
     4681 gtcttcaact ttttgtcgcc cctgaactgg ctcgagatac cctttatcga taaacccttt
     4741 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     4801 tccggcccga atattcccca cggtcaccat cttgtcacca atttgcgccc agaccgcagc
     4861 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     4921 catcagcagg agaataacag cttgtttata catgaaaaaa taaccgaggg tgaaacaacg
     4981 cttttattgt agcaaaagcg ccatcagaat cgcgggttaa tactcataca aattatgttt
     5041 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5101 cagttcgaca atacactcac gaaactctga actggtcatg gtttcacggc aagccatcat
     5161 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5221 taataatgaa gccacgcgat acggtggcgc tccggctata tcggttaaga agatgacacc
     5281 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5341 taatccgcgc cagaaattca cggcccggca ctgaagaagt gccccatgtt ttttctccag
     5401 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5461 cttcctcctt agccgaaaat cagcctaccc tgaccattgt tgataaaggg tgatagccgt
     5521 caaaactcct ctcccccgaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5581 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     5641 ccaaaggtag tgaccagcgg cacatcattt ataccgagga agccgtagcg gaaaccactg
     5701 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     5761 agtgagtaaa agaccccgcc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     5821 ctgatgtcat cgaacgtttt ggcaaacacg ccgttcagca aacccgcaag ggagaatagc
     5881 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     5941 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6001 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6061 ttacgctgga acttggcacc aaaaaatgat gacgcgacgt tagcgtaggc gttggtgatc
     6121 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6181 ccaatacgcg aacctatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6241 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6301 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6361 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6421 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgtagc acttcaactt
     6481 ccagcgtcgc agtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6541 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6601 aggtattttc caccagctca ccatgttgaa taatgccgat attgaggcac agcatttctg
     6661 cttcttccag gtagtgtgtg gtaagaatga tggtggtgcc tttgtcgttt aaatccttca
     6721 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     6781 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     6841 cagataacat acgcgcacgt tcgttgcgtt ttccccataa atcgagctgc ttaagatatt
     6901 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     6961 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7021 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7081 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7141 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7201 gaacgccgcc tggatagttt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7261 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7321 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7381 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7441 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7501 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7561 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7621 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     7681 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     7741 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     7801 gtgtgaactg aacgtcatgg aacaggtgta taacctgggc cactccacca ttatgcaatc
     7861 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     7921 cttgctgcgt gatctggatg ttaccgccac caaccgcgaa acccttgagc aacgttaccg
     7981 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8041 atgcaacaca tccggcaact tcacacttac tcgtccagca gaatcacttt gccaatatac
     8101 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8161 atcgagaaac cgataaattc aaccgggacg ttcacttcac gacgggacgg tttatccagc
     8221 agcgtacaaa tcgccaacga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8281 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8341 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8401 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8461 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8521 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8581 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     8641 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     8701 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     8761 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     8821 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     8881 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     8941 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa acgcgccgcg
     9001 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9061 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9121 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9181 gtggtcgcac tgctgattag cggtggtatc ctgacctggg ccggatttaa cgatccgcag
     9241 gagatcaacg gcaccttaag cgccgatgcc acacctgccg aagctatctc ccccgttgcc
     9301 gatcaggact ggcctgcgta tggccgcaat caggaaggtc aacgcttttc accactgaag
     9361 caaattcacg ccgataacgt ccacaagctg aaagaagcct gggtgttccg tactggcgat
     9421 gtgaagcagc cgaacgatcc gggtgaaatc accaatgaag tgacgccgat taaagtgggc
     9481 gatacccttt acctgtgtac cgctcaccag cgcctgtttg cgcttgatgc cgccagcggc
     9541 aaagagaaat ggcattacga tcctgagctg aaaaccaacg agtctttcca gcacgtaacc
     9601 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
     9661 gattgcccgc gtcgtatcat tcttccagtg aatgatggtc gcctgattgc gattaacgct
     9721 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
     9781 atgccagaca ccaaaccggg tctgtatgag ccgacttctc cgccaattat caccgataaa
     9841 accatcgtga tggccggttc agtcaccgat aacttctcaa cccgcgaaac gtctggcgtg
     9901 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc gggcgcgaaa
     9961 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcacc aaactcgtgg
    10021 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10081 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10141 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10201 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10261 gtgccgatta tttacgctcc ggcgaaaacc ggtaacattt ttgtgctcga tcgtcgtaat
    10321 ggcgaactgg tggttccggc cccggaaaaa ccggttcccc aaggtgctgc caaaggcgat
    10381 tacgttaccc ctactcaacc attctcggaa ctgagcttcc gtccgacgaa agatttgagc
    10441 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10501 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggtacgct ggtcttcccg
    10561 ggtaacctgg ggatgttcga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10621 attgccaacc caatggcact gccgtttgtt tcgaaactgc tcccgcgtgg tcctggcaac
    10681 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    10741 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccatttgg tctgccatgt
    10801 aaacagccag catggggtta tatctcggcg ctggatctga aaactaatga agtggtgtgg
    10861 aagaaacgta ttggtacgcc gcaggacagt atgccgttcc cgatgccggt tccggtgccg
    10921 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    10981 atcgccgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11041 tggcagggtc gtttaccggc gggtggtcag gcaacaccga tgacctatga agtgaatggc
    11101 aagcagtacg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11161 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagaaatcc
    11221 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11281 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11341 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11401 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcgtg ccgtgaatat
    11461 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11521 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11581 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccatgc cccatatggc
    11641 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    11701 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    11761 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    11821 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggtgcaatcg
    11881 ccatccccat ctggctgacc ggcagcgtca ccaggtcaaa gggtttgttg tcgttaacct
    11941 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12001 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12061 aattgagcga gcgggcatta cagccattga gcaaacgcag gcgcagccaa ccacgcgggg
    12121 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12181 cggcggtcat cacatccagt tgataatcaa tctgcccgtc ggcgctaaat ttcttatcct
    12241 gaacgatcac cggaacatca tcaatacccc actgttttgg cagcattaat ttcaggatct
    12301 cgtcatcttc aatcaccacc agaccagcca gccccatcgc cacctgtcgc cggtcttgcc
    12361 atgttgatgt ggatggaacc agcaggtagc ggcaggttga tcaacgttca acgtcaccga
    12421 gcgcttgcca cctggcggaa taattccctg tgggccgccg tcgacgtcac ccggtacttc
    12481 cagcccgtgc cagtgcaacg tcgtctcttc cgccagttgg ttgtagatat caaccgttac
    12541 cgctttgccg cgctgtaatt tcaccgccgg ccccagcaga ttgccgttat agccccaggt
    12601 agttgcagtt ttctcgccaa aggtggactg acctgcgcca atagttaact gaatgcgatt
    12661 acgggcatcg gtcgtgagca aatcagggat cggtaacgtt gggcgttccg ccgcaaagac
    12721 tgcacggctc cacagtggca aggctgaagc cacacccagt gcgacggaat atttcaagaa
    12781 atcacgacgt tgcatagtta tttccttatt cttaagcagg cgaaacaaaa tcaaacgttg
    12841 agcatagtcc ttccccttac gggaaggtca agccgcagac acaatttaat cgacaataat
    12901 ggtcgtcgcc tcgctcgcag tgtgctaacg tttatcttct ttaagcccct ggtagaagca
    12961 atgaagacgt ttttcagaac agtgttattc ggcagcctga tcgccgtttg cgcaaacagt
    13021 tacgcgctca gcgagtctga agccgaagat atggccgatt taacggcagt ttttgtcttt
    13081 ctgaagaacg attgtggtta ccagaactta cctaacgggc aaattcgtcg cgcactggtc
    13141 tttttcgccc agcaaaacca gtgggatctc agtaattacg acaccttcga catgaaagct
    13201 ctcggtgaag acagctaccg cgatctcagc ggcattggca ttcccgtcgc taaaaaatgc
    13261 aaagccctgg cccgcgattc cttaagcctg cttgcctacg tcaaataatc cctgatacct
    13321 ttttgtagaa atattgaccg tgcatccgcg gtcaatgtta gctattatgt tgcgcccttt
    13381 ttttacgggt gttaacaaag gaggtatcaa cccatggccg aaaaaaaaca gtggcatgaa
    13441 acgctacacg accagtttgg gcagtacttt gcggtagata acgttctgta tcatgaaaag
    13501 accgatcacc aggatctgat catttttgag aacgctgcat ttggtcgcgt aatggcgctg
    13561 gatggcgtag tacaaaccac cgagcgcgac gagtttatct atcatgagat gatgacccat
    13621 gttccgctac tggcccacgg tcacgcgaaa catgtgctga ttatcggcgg cggcgacggt
    13681 gccatgctgc gtgaagtaac ccgacataaa aacgttgagt caatcacgat ggtggaaatc
    13741 gatgcgggtg tcgtgtcgtt ctgccgtcag tatctgccca accataacgc cggtagctac
    13801 gacgatccgc gctttcaact agtgatcgac gatggcgtca atttcgttaa tcaaaccagc
    13861 cagacctttg atgtcattat ctccgactgc accgatccta tcggtcccgg cgaaagcctg
    13921 ttcacctcag cattttatga aggctgcaaa cgttgcctga atcctggcgg tatcttcgtc
    13981 gcacaaaacg gcgtctgctt tttacagcag gaagaagcca tcgacagcca tcgcaaactc
    14041 agccattact tcagcgacgt tggcttttat caggcggcga tcccgaccta ttacggcggt
    14101 atcatgactt ttgcatgggc gacagataac gacgccttac gccatctctc aaccgaaatt
    14161 attcaggcgc gttttctcgc ttctggcctg aaatgccgtt attacaatcc ggcaatccat
    14221 acggcagctt ttgccttacc tcagtatctg caagacgcac tggcttcaca gcagtcctaa
    14281 ggagaagata agaaattgaa aaaactgaaa ctgcatggct ttaataatct gaccaaaagt
    14341 ctgagttttt gtatttacga tatctgctac gccaaaactg ccgaagagcg cgacggttat
    14401 attgcttata tcgatgaact ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc
    14461 tgttccatta tcggggccaa tattcttaac atcgcccgcc aggattacga accacagggt
    14521 gccagcgtca ctattctggt gagtgaagaa ccggttgacc cgaaactcat cgacaaaaca
    14581 gaacaccccg gcccactgcc agaaacggtg gttgctcatc tcgataaaag tcatatttgc
    14641 gtacatacct acccggaaag tcatcctgaa ggcggtttat gtaccttccg cgccgatatt
    14701 gaagtctcta cctgcggcgt gatttctccg ctgaaggcgc tgaattacct gatccaccag
    14761 cttgagtccg atatcgtaac cattgattat cgcgtgcgcg gttttactcg cgacattaac
    14821 ggtatgaagc actttatcga ccatgagatt aattcgattc agaactttat gtctgacgat
    14881 atgaaggcgc tgtatgacat ggtggatgta aacgtctatc aggaaaatat cttccatacc
    14941 aagatgttgc ttaaagagtt cgaccttaag cactacatgt tccacaccaa accggaagat
    15001 ttaaccgaca gcgagcgcca ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt
    15061 tattacgggc gcaatatgcc agctgtttaa cggctctggc ggagctccca ggctccgcca
    15121 gatttattta cttctgctgc acgaaattgc ggtaagccgc cacgacctgc agaaaatcct
    15181 caacgccgca tagcgacagg ctttcttcgt cgtagtagtt catcccctct tccatttcat
    15241 cgccagcgaa ttccagttga ttggcgcgaa ccatcacctc ttcagcgtcc atccacaggg
    15301 tgtattcatg ccctgcccgt tgccaggacc gttcgctacc tttcagtgcg tgcgctgctt
    15361 gttccacttc atcaagcaag gccaggtttt ctttcacctc ttcattaaac cagtgcccga
    15421 ccacttcatg ccccatggac atacgcacct ttaccactcc ggtaatatcg cgcagaaatt
    15481 cgtaatccat agtgtgttcc tctgctcccg gcaatgcgcc gaaatcgcat tgcgccattg
    15541 atgtaattat cgcagcctta ccggagaaaa acagcggaac ggcgatggtt tagaaataaa
    15601 aaatgcccgg gaatctcatt caccgggcat tggtgtcgtt tatgcgcagc gcgtgcgctt
    15661 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    15721 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    15781 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    15841 gcagaagcca ggaagacatt cgcgccagta cccaaacggt tcgggaagtt acgggtagag
    15901 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagggaacag
    15961 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttccgtc
    16021 agttgcgcgg cgtccatacg ggttggcggt gccacccaca ggcgggtcgg caactgacct
    16081 ttgtgcgcat ccagcagttt accagcggca cggaagtgac cgatgttagt catgcaagaa
    16141 ccgataaata cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcatcc
    16201 ggatcgttcg gagcacacag gattggctct ttaatctccg ccagatcgat gtcgatcact
    16261 gccgcgtatt ccgcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16321 atgccctgaa tacggcgttc cagggtacga cgatcgccgt aaccttccgc gatcatccac
    16381 ttcagcagga cgatgttgga gctcaggtat tcgatgatcg gttctttgtt cagcttgata
    16441 gtacaaccag cggcagaacg ctcggcggac gcatcggtta gctcaaaggc ctgctcaact
    16501 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16561 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcggaatggc gtgtaccaga
    16621 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    16681 atatccagcg gcattacgcc agttgcggca gcaaacgcca ccagaccaga acccgccggg
    16741 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    16801 agcagcatac ggttcagcca ggagtgaatg acgccgtcac ccggacgcag cgacacaccg
    16861 ccacggttaa taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    16921 tacgccgcgg tgtggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    16981 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgagagcc tacagaagtc
    17041 attttcggtt cgcagtacgc gcccggacga atgcctttca cgccacaggc acggcctacc
    17101 attttctgcg ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17161 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17221 atcagcggaa tacggccgcc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17281 aaggtcgcca gcagttcgcc tgtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17341 tcaatcacgt cgcccatgtt caggttagag acgttgactt cgattggcag tgcacccgcg
    17401 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17461 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17521 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17581 tgttgcagag cttcaatctg tttgatcggg ccaacaacac ctggctggtc tggctcaata
    17641 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgtgaccac
    17701 gcatccggtg ccggagaaag gtcatcggtg ttggtttcgc cagtgacttt aaagacggta
    17761 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    17821 tgcattacct gcttcgcata ttcgttgcct gctttcgctt tctcttctac gtcatagaag
    17881 ttatcgaaca tcagcagcgt gtgagaaagt gctttggcag caataggtgc cagtttggca
    17941 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt gcccagcagt
    18001 tcgatagctt tttccggagt cagcagaggg gatttagctt cgcctttagc gatagcagcc
    18061 aggaagcctg cttttacata ggcggcttca tcgacgcctg ggggaacacg gttggttaac
    18121 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagcgcg
    18181 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18241 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18301 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18361 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18421 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18481 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18541 cgcctcttta tactcggatt cacagcacct gcgggtggca gttcgccgac cattgcgatt
    18601 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcgctaa
    18661 tttgcagtgc cgggctttgt gctgcctctg ccgggctata tataaaaagc cgcacagtgg
    18721 aagcgcctgt agaaccgcaa tcgacacaac tggctgtgtc tgacgctgcc gctgttacgc
    18781 ttcctgcaac ggtttccgca cctcccgtaa tacccgcagt cgtcaaatcc gcattcagca
    18841 ctgcacaaat agatcaatgg gtcgcacccg tcgcgctgta tcccgacgcc ctactttcgc
    18901 aggtgctgat ggcatcaacc tatccgacaa acgttgctca agcagtgcaa tggtcgcacg
    18961 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19021 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19081 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19141 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19201 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19261 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19321 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19381 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19441 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19501 acgcactatt cagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19561 atgatgatta tcatcaccac gatggcggtc atcgtgacag taatggctgg caacacaacg
    19621 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    19681 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    19741 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    19801 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    19861 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    19921 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    19981 tgacccagca gcaaaaggac gccgctcgtc agcgttatca gtcggcctct cctgagcagc
    20041 gccaggcagt ccgcgagaaa atgcagacta acccgcagat ccagcagcga agggaggcag
    20101 cacgccagcg tattcagtcc gcctcgcctg agcagcgcca ggcggttcgc gagaaaatgc
    20161 agactaaccc gcagaaccag cagcgaagag aggcagcgcg tgagcgtatt cagtctgcaa
    20221 ctcccgaaca gcgccaggtg tttaaggaaa aagtacagca gcgcccactg aaccaacagc
    20281 aacgtgataa cgcccgccag cgtatacaat cggcatcacc tgaacaacgt caggtttttc
    20341 gggagaaagt tcaggagagc cgcccacaac gtctaaacga cagtaaccat actgtcaggc
    20401 tgaataacga gcaacggtca acagtacgcg aacgtctctc tgaacgtgga gcaagaagac
    20461 tggaaaggta aattgcagac gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg
    20521 gatgttccgg caaacgaaaa attacttctt cttcgctttc gggttcgggt tcgggttcgg
    20581 gttcggcagg tcggtaatgc taccttcgaa cacttctgcc gccaggccca cagactcgtg
    20641 cagagtcggg tgcgcgtgga tggtcagtgc gatgtcttca gcatcacaac ccatttcgat
    20701 tgccagcccg atttcaccca gcagctcgcc gccgttggta ccaacaatcg caccaccgat
    20761 cacgcggtga gattctttgt cgaaaatcag cttggtcata ccgtctgcgc agtcagaagc
    20821 gatagcacga ccagaagcag cccacgggaa ggtggcggtt tcatagctga tgcctttctc
    20881 tttcgcttct ttctcagtca gacctaccca tgcaacttct ggttcggtat aggcgatgga
    20941 cgggataact ttcggatcga agtagtgttt cttaccggcg ataacttcag cggcaacgtg
    21001 accttcgtga acacctttgt gtgccagcat cggctgaccg acgatatcgc cgatagcaaa
    21061 gatgtgcggt acgttggtac gcagctgttt gtcaacgcgg atgaaaccac ggtcgtcaac
    21121 ttccacgcca gctttgcctg cgtcgaggtt tttaccgttc ggcacacgac caatcgctac
    21181 cagcacggcg tcgtaacgct gcggttcagc tggtgctttt ttgccttcca tcgtcacata
    21241 aataccgtct tctttcgctt caacggcggt aactttggtt tccagcatca ggttgaattt
    21301 cttgctgata cgcttggtga agactttaac gatgtctttg tcagctgccg ggataacctg
    21361 gtcgaacatt tcaaccacgt caatctgtga acccagcgcg tggtaaacgg tgcccatttc
    21421 cagaccgatg ataccgccac ccattaccag caggcgttct ggcacttctt tcagttccag
    21481 cgcgtcagtg gagtcccaga tacgcggatc ttcatgcgga ataaacggca gttggatcgg
    21541 gcgagaaccc gctgcaatga tcgcattgtc gaagttgatc acggttttgc cgttctcacc
    21601 ttcaacttcc agggtgttag ccccggtgaa tttacccaga ccgttgacca ctttgacttt
    21661 gcggcctttc gccataccag ccagaccacc ggtcagctga ttgatcactt tctctttcca
    21721 ggtacgaatc ttgtcaatat cggttttcgg ttcgccgaag acgataccgt gttcagccag
    21781 cgctttggct tcttcgataa cttttgctac gtgcagcagt gctttagaag ggatacagcc
    21841 gacgttcagg caaacaccgc caagggtgtt gtaacgttct acgattacgg tttccagacc
    21901 taaatcagcg caacggaagg cagcggagta acctgcgggg cctgccccaa gtaccacgac
    21961 ctgagttttg atttcagtac tcatcatgac ctctatatat ttatctccgg cggtcatacc
    22021 cgtcgtcttt caggccgcag cggcgttagc ttcacgccct caccccagtc acttacttat
    22081 gtaagctcct ggggattctg gcgcttgcta ccttgctgca cccagaaatc catagggtat
    22141 gtgaagtaat accctaacca ccaccgggtc gttctatccg tcggtatttt acaaaattgt
    22201 taacaatttt taaacaacaa acggcaaccg atttgtctat tcgctaataa cctcaataca
    22261 ttcatgagat taccagaaaa aagccggccg ttgggccggc tcttttactt acatcaccag
    22321 acggcgaatg tcagacagcg tgttgttaat gatggtaatg aaacgggcac catcagcacc
    22381 gtcgatcacg cggtggtcga aggagagaga aatcggcagc atcagacgcg gcacgaactc
    22441 tttaccattc cacaccggct ccatcgcgga cttggaaacg ccgaggatag ccacttccgg
    22501 cgcgttcaca atcggcgcga agtgggtagt acccaggccg ccgatgctgg agatggtgaa
    22561 gcaaccgccc tgcatttcgc ccgcagtcag cttaccgtca cgcgctttct tagaaatagt
    22621 catcagctcg cgagacagct cgatgatgcc tttcttgttg acgtctttga ataccggaac
    22681 aaccagaccg ttcggggtat ccaccgccac accgatgttg atgtatttct tcagggtcag
    22741 acgctgaccg tcttccgaca gcgaactatt gaagcgaggc atctgctcaa gagctgcagc
    22801 aacggctttc atgatgaaga caaccggggt gatcttcaca tccagcttac gtttcgccgc
    22861 ttcttcgttc tgctgtttac ggaacgcttc cagctcggtg atatcggttt tgtcgaagtg
    22921 ggtaacatgc gggatcatca cccagttacg gctcaggtta gcaccagaaa ttttctggat
    22981 acggcccagt tccacttctt cgatttcgcc aaacttgctg aagtccacct tcggccacgg
    23041 cagcatgcca gggataccac cgccagtcgc cgccggagct gcttctgcac gtttgatagc
    23101 ttctttcacg taagcctgaa cgtcttcgcg caggatacga cctttacggc cagtgccctt
    23161 cactttcgcc aggttaacgc caaactcgcg tgccagacgg cggatcagcg gggtcgcgtg
    23221 aacgtaagcg tcgttttcag caaattcaga tttgccttcc gctttcgcag ccggagctgc
    23281 tgccggagct tcagcttttg ctgccggagc cggcgctgcc gcttcctgtt tcgcaggagc
    23341 tgccgcaggc gctgcgcctt caacttcgaa gatcataatc agcgaaccag ttttcacttt
    23401 atcgccaacg ttgactttca gttccttcac gacgcctgca aacggagccg gaacttccat
    23461 agaagcttta tcgccttcta cggtaatcag tgactgttca gcggcaactt tgtcgcccac
    23521 tttcaccatc acttcggtca cttcaacttc gtcaccgccg atatccggaa cgttaacttc
    23581 tttcacgcca gctgctggtg cgggggtcgc tgccggagtc gcttcctgtt tagccgccgg
    23641 agctgccgcg cctgcttcac ccgcgacttc gaagaccata atcagcgagc cggtagacac
    23701 tttgtcaccc acgttcactt tgatctcttt cacggtacct gcaaacggag ccggaacttc
    23761 catagaagcc ttgtcgcctt ctacggtgat cagcgactgt tcagcttcaa ctttatcgcc
    23821 aactttcacc aggatttcgg tcacttcaac ttcgtcgctg ccgatatccg gaacgttaac
    23881 gtcttttgcc gccgcagccg cgggtgctgc tgccggagct gcttctttct tctcttctgc
    23941 ctgagcaggt gcagcgtctg ctgcaccgtc ggcggaatcg aaaatcataa tcagtgcgcc
    24001 ggtctgggtt ttatcgccaa cagagacttt gatctcttta acgatacccg cctgcggaga
    24061 tggaacttcc atagaggctt tgtcgccttc tacggtgatc agcgactgtt cggcttcaac
    24121 tttgtcgccc actttgacca ggatctcggt gatttcaact tcatcagccc cgatgtccgg
    24181 tactttgatt tcgatagcca ttattctttt acctcttacg ccagacgcgg gttaacttta
    24241 tctgcatcga tgttgaattt ggcgattgcg tcagcaacca ctttcttatc gatttcgcca
    24301 cgtttagcca gttcgcccag cgccgcaacc acgacataag aagcatcaac ttcgaagtgg
    24361 tgacgcaggt tctcacggct gtcggaacga ccgaagccat cagtacccag tacgcgatag
    24421 tcgtcagccg gtacgtaagt acggacctgc tcagcgaaca gtttcatata gtcggtagat
    24481 gccactgccg gcgcgtcgtt catcacctga gcgatatacg gaacgcgcgg agtttccagc
    24541 ggatgcagca tgttccagcg ttcacaatcc tgaccatcac gcgccagctc ggtgaaggag
    24601 gtcacgctat aaacgtcaga acctacgccg taatctttcg ccaggatctc tgccgcttca
    24661 cggacgtgac gcaggatgga accggagccc agcagctgaa ctttaccttt gctaccttca
    24721 atggtttcga gtttgtagat acctttacgg ataccttcct cagcaccttc cggcattgcc
    24781 ggcatgtggt agttttcgtt cagcgtagtg atgtagtagt aaacgttctc ttgtttttca
    24841 ccgtacatac gctccagacc gtcatgcata atgacagcaa cttcgtaagc gtaagccggg
    24901 tcgtaagaga tacagttcgg gatagtcagc gactgaatgt ggctgtgacc atcttcgtgc
    24961 tgcagacctt caccgttcag ggtggtacga ccggaagtac cgccgatcag gaagccacgc
    25021 gcttgctggt ctccagccgc ccagcacaga tcgccgatac gctggaagcc gaacatcgag
    25081 taatagatgt agaatgggat catcggcaga ttgttcgtgc tgtaagaggt cgccgctgcc
    25141 agccaggaac accctgcgcc cagctcgttg atcccttcct gcagaatctg acctttctcg
    25201 tcttctttat agtaagcaac ctgctcgcgg tcctgcgggg tgtactgctg accgttcggg
    25261 ctgtaaatac caatctgacg gaacagacct tccataccga aagtacgcgc ttcgtcggcg
    25321 atgatcggta ccagacgatc tttgatcgac ttgttcttca gcatcacgtt cagagcacga
    25381 acaaaagcga tagtggtaga gagctctttg ctctgctctt ccagcagcgc gccgaagtct
    25441 tgcaggctcg gcagctcaag cttctcggtg aagttcggct gacggcttgg cagataaccg
    25501 tgcagtttct gacgctgagc gtgcagatag gtatgctctt cagaaccttc cgggaaggtg
    25561 atgtacggca gtttttcgat atctgcatca gacaccggca cattgaaacg gtcgcggatg
    25621 tgacgcacgc cgtccatgtt cattttctta acctggtgcg cgatgttttt accttcagcc
    25681 gcgtcgccca tgccgtaacc tttaatggta tgagcaagga ttactgtcgc tttgcctttg
    25741 gtttcctgcg ctttcttgaa tgcagcgtag attttcttcg gatcgtgacc accacggttc
    25801 agtgcccaga tctgctcgtc agtccagtct gcaaccagtg ctgcggtttc aggatattta
    25861 ccgaagaagt gttcacgaac gtacgcacca tctttcgatt tgaaggtctg gtagtcgccg
    25921 tcaacggttt cgttcatcag ctggatcagt ttaccgctgg tatctttacg cagcagttca
    25981 tcccaacggc taccccacat cactttgatc acgttccagc cagcaccttc gaagatgcct
    26041 tccagttcgt tgatgatctt gccgttaccg gtgaccgggc cgtcaagacg ctgcaggtta
    26101 cagttgataa cgaaaaccag gttatccagt ttttcacggg tagcgatggt gatcgcacct
    26161 ttggattccg gttcgtccat ttcaccgtcg ccgaggaacg cgtaaacggt ctgtttagag
    26221 gtatctttca ggccacggtg ttccagatat ttcaggaatt tagcctggta aatagcacca
    26281 atcggaccca gacccataga tacggtcggg aactgccaga attccggcat cagtttcggg
    26341 tgcggatagg aagagaggcc attgccgtga acttcctgac ggaagttatc cagctgctcc
    26401 tgagtcagac gaccttccag gaaagcacgg gcgtaaacgc ccggggagat gtggccctgg
    26461 aagtaaacca ggtcgccgcc atcctgctcg ttgcgtgcac ggaagaagtg gttaaagcac
    26521 acatcataaa tggttgcgga agactggaag gacgccatgt ggccgcccag ttcgaggtct
    26581 tttttcgacg cacgcagcac cgtcatgatg gcgttccagc ggatagctga acgaatacgg
    26641 cgttccagtt ccagattacc cggatactcc ggttgttctt caacggggat ggtgttgatg
    26701 tagttgctga tacctgtgcc tgcggctacg tttacaccgc ctttgcgggc ttcagcaagc
    26761 agttggtcga tcagatactg agcacgctca acaccttctt cacggatgac cgattcgatc
    26821 gcctggagcc agtcgcgagt ttcgatcgga tccacgtcat ttgggaaacg ttctgacatg
    26881 ggttattcct tatctatcta ataacgttga gttttctgga acctgtccca ttgaactctc
    26941 gccagaaagc tcaataagac aggttctacg tttagttacc gcgcttttat atgcgcttga
    27001 tttacaacat cttctggata atttttacca gaaaaatcac taattctttc gttgctccag
    27061 acgacgcaga gaacgctcac ggcggctctc ttcacggctt ctgtcgagca aaatttcttc
    27121 gataaaggcc agatggcgat gcgatgcttc gcgcgcttct tccggcttac cggccataat
    27181 cgcttcaaat atgcgggtgc ggtgactact caccagcggc agcatctcgc gacgcgaata
    27241 gagcaattcg aagttctggc ggacattctg ggccaacatc ggctccatac accttagcag
    27301 atgaagcaga accacattgt gggccgcttc ggtgacggca atctgatact ggagtacggc
    27361 gtttgattcc gcgtccagat cgccagactg ctgcgccagc tctatggcgt ggtggagttc
    27421 acggatgcgt tccttgtctt catcggtact acgcagcgcg gcgtaataag cggcgatacc
    27481 ttccagggcg tgtcgtgttt cgagcaagtc atactgtgat tcaggatggt cggagagcag
    27541 ctccaccagc ggatcgctga agctttgcca taggctgctc tggacaaaag tgccgccacc
    27601 ctggcgacga agcaacaagc ccttcgcttc gagacgttga atcgcctcac gcaaggaggg
    27661 acgggagacg tcgaactgtt ttgccagttc gcgttccggt gggagttttt cgcccgggcg
    27721 gagagtgcct tcgaggatca aaaactccag ttgctgctca atcacatcgg agagttttgg
    27781 ttggcggatt ttgctgtagg ccatgagttc ctgtcttaag ccacttgccg aagtcaattg
    27841 gtcttaccaa tttcatgtct gtgacgctaa agtaacaaag tattcacctt atgtccatac
    27901 aggttttgat tgaaatcatg aaactgtgca cattttaaca acttgacata tataacgttt
    27961 caaagttgta actatgcaca aatgtagact ttacgtagga aagggatttt ttaacgatta
    28021 ttaaactata aaaatccgaa ttgaaccgat tcacttacca attttttgat ttttaagtca
    28081 ataaaaacga atttaaattc actctgcata ttgagagggg ttgaggctga gctttacaaa
    28141 cggtttcttt ttaagcaact catcttcaac catgcataaa gcgggtgcat tcgctgccgc
    28201 ataccattat tcttgatctg acggaagtct ttttgtaaca attcaaactt ccttgatgta
    28261 aataaatcaa tacaacaaac ggaattgcaa acttacacac gcatcactgc gtagatcaaa
    28321 aaaacaatca ccgcacgagg tttcatgatg gaaggtcaac agcacggcga gcagctaaag
    28381 cgcggcctta aaaaccgcca tattcagctt atcgcgctgg gtggcgcgat agggacaggg
    28441 ctattcctgg gtagcgcctc cgtaatacag tccgcagggc cagggattat cctgggttac
    28501 gccattgctg gttttatcgc ctttctgatc atgcgtcagc tgggtgaaat ggtggtcgaa
    28561 gaacctgtcg caggctcctt tagccacttt gcttataaat actggggcag ttttgccggt
    28621 ttcgcctctg gctggaacta ctgggtactg tacgttttag ttgccatggc agagctgact
    28681 gccgtgggta aatacattca gttctggtat ccggaaatcc caacctgggt ttctgccgcc
    28741 gtattctttg tggtgattaa cgccatcaac ctgaccaacg ttaaagtgtt tggcgagatg
    28801 gagttctggt ttgccattat caaagttatt gcggtagtag cgatgatcat cttcggcggc
    28861 tggctgctgt tcagtggcaa cggcggcccg caggcgagcg ttagcaacct gtgggatcaa
    28921 ggtggtttcc tgccgcacgg cttcaccgga ctggtgatga tgatggcgat tatcatgttc
    28981 tcgttcggtg gtctggaact ggtggggatc accgcagcag aagctgataa cccggagcaa
    29041 agtatcccga aagcaactaa ccaggttatc taccgcatcc tgattttcta tattggctcg
    29101 ttagccgttc tgctctcact gatgccgtgg acccgcgtta ccgccgatac cagtccgttt
    29161 gtgctgatct tccacgagtt aggcgatacc tttgtggcga atgcgctgaa catcgtggta
    29221 ctgactgcgg cgctctccgt gtacaacagc tgcgtatatt gcaacagccg tatgctgttt
    29281 ggtctggcac aacaggggaa cgcgccaaaa gcgctggcgt ctgtcgataa acgcggcgta
    29341 ccggttaaca ccattctggt gtctgcgctg gttacagcat tgtgcgtatt gattaactat
    29401 cttgctccgg aatccgcatt tggcctgtta atggcactgg tggtatccgc actggtgatc
    29461 aactgggcga tgatcagtct ggcgcatatg aagttccgtc gcgccaagca ggaacaaggc
    29521 gtggtaactc gcttccctgc tctgctttat ccgctgggta actggatctg cctgctgttt
    29581 atggcggcgg tactggtgat tatgctgatg accccaggaa tggcgatttc ggtatacctg
    29641 atcccggtat ggctgatcgt gttaggtatc ggctatctgt ttaaagagaa aaccgccaaa
    29701 gccgtaaaag cgcattaatc tctctacgcc ctcacccgta cagggtgagg gcaataatct
    29761 ctacaccaac gccccgtaaa tcgtcagtag cgcaatcacc accacgacca cgaacgaggt
    29821 tttcttcgcc attgaaaccg ctgccttcgg cgtctccacc ttatcgacat gcggttcacg
    29881 cgccagagag aactgcgcca gacgcgttaa tacctgatat tgcgaagtat ggaaatcacc
    29941 cagcgaagca aaccaggccg gtaacgcttt ctcaccatga ccgatcaagg catataccac
    30001 acccgcaaga cgaaccggca cccaatccag tacatgaagc actgcatcaa tgccggactg
    30061 taaacgatga tgcggcgtct ggtatctcgc cagccagtat tgccatgcac gcaagaacgc
    30121 ataccccatc agcgtaacgg gtccccaggt tccccccaca atcagccaga acagcggtgc
    30181 aagataaaaa cgaaagttaa tccacagcaa tgcattttgc agctcacgca aatactcacg
    30241 ttcgtcgcag cctgccggga cgccgtgaat catggtgagt tcgccagcca tcgtggcacg
    30301 ggcatggcta tcattacgtg aagcagctgt cagataagca tgataatgaa gacgaacttt
    30361 acctgcgcca atacacagca aaccaatcag cagccacacc agcagcgtgg gaacgttgaa
    30421 caatactccc tgcaatgcgc gtaacagtaa aaaagtcaca cccatcgcaa taatggtcat
    30481 gcctaacgtg cgcccgagag aaaaatgttt cacccgccga aagaacgctt caagacgatg
    30541 atcaagctgc cagtgctcgc ccaacttaaa caggcgctcg aaaattaaca ccagtaaggt
    30601 tgtaaatagc gtcatgttgt ctccttgctg accagcgcac gaaaccgtgc ccaatcaaat
    30661 gcaggaccgg gatcggtttt ccgatccggc gcaatatcac aatggcccgt catgttatta
    30721 gcgatatcgg gatagcgatc tatcagtgcc cgcgtaaccg ccgcaagctg ttgatactgc
    30781 gcatcggtat acgccagagt atcggtgcct tcaagctcaa tcccaataga aaaatcattg
    30841 cagcgttcac gcccctgata ctgagagact cccgcatgcc aggcacgttt atcgaaagga
    30901 acatactgga ctatttcacc atcacggcga atcaaacagt gagcggagac gcgcaaatgg
    30961 gcgatctcag caaagaaagg atgtgcctgc ggatcaatag ttccagtgaa taatgcgtcg
    31021 atccacggac cgccaaactc gcctggcggc aggctaatat tgtgcaccac cagcagggtg
    31081 ggtgtttcgt catccaggcg gcaatcgtaa tgtggtgagg gaacgcggcg cgcgccaacc
    31141 agccacccct gttctaacaa catgcaggat ctccttatat gtggtgctaa tacccggttc
    31201 agagtagcat gtttctacct tatgattcgt tagctatctg gagttttaac atgccgcctc
    31261 gccgctacaa ccctgacacc cgacgtgacg agctgctgga acgcattaat ctcgatatcc
    31321 ccggcgcggt ggcccaggcg ctgcgggaag atttaggcgg aacagtcgat gccaacaatg
    31381 acattacggc aaaactttta ccggaaaatt ctcgctctca tgccacggtg atcacccgcg
    31441 agaatggcgt attttgtggc aaacgctggg ttgaagaggt gtttattcaa ctggcaggcg
    31501 acgatgtcac cataatctgg catgtggatg acggcgatgt catcaacgcc aatcaaccct
    31561 tgttcgaact tgaaggccca tcccgcgtgc tgttaacggg cgaacgcact gcgcttaatt
    31621 ttgtgcaaac cctttcagga gttgccagta aggtacgcca ctatgtcgaa ttactggaag
    31681 gcaccaacac gcagttgttg gatacgcgca aaaccttacc tggcctgcgt tcagctctga
    31741 aatacgcggt actttgcggc ggcggagcga atcaccgtct ggggctttct gatgccttcc
    31801 tgatcaaaga aaaccatatt attgcctccg gctcagtgcg ccaggcggtc gaaaaagcgt
    31861 cctggctgca tccggatgcg ccagtagaag tcgaagtaga gaatctggaa gaacttgatg
    31921 aggccctgaa agccggagcc gatatcatca tgctggataa cttcgaaaca gaacagatgc
    31981 gcgaagccgt caaacgcacc aacggcaagg cgctactgga agtgtctggc aacgtcactg
    32041 acaaaacact gcgcgaattt gccgaaacgg gcgtggactt tatctccgtc ggcgcgctaa
    32101 ctaaacacgt acaagcactc gacctttcaa tgcgttttcg ctaattgctg acagatttat
    32161 ccgctccatc gcgggcggat aatctcccct tccccaactt tcttcgtaac gcctcgcaaa
    32221 tttcttgtct ttcgctgcaa cgaaataaaa aacctcggaa tctgctttcc cgtttcgttt
    32281 tttgcccctc gcctgtgctg atggcgtttg ccaaagtagc accaaccaaa tcaaggagcg
    32341 aaacagatgg acaagcaaca cggttttaca cttatcgaac tgatggtggt tattggcatc
    32401 attgccattt taagcgccat tggtattccc gcttatcaaa actacctgcg caaagccgca
    32461 ctcaccgaca tgctacaaac ctttgtgcct taccgtaccg ccgtagagtt gtgcgcgctg
    32521 gaacatggtg gattagatac ctgcgacggt ggcagcaatg gcattccctc gcctaccacc
    32581 acccgctatg tttcagccat gagtgtggca aagggcgtgg tgtcgctgac cgggcaagaa
    32641 agtctcaatg ggctaagcgt cgtcatgacg ccaggttggg ataacgcaaa cggcgtcacc
    32701 ggctgggcgc gcaactgcaa tattcaaagt gacagcgcat tgcagcaagc ctgcgaagat
    32761 gtcttccgct ttgatgacgc caactaagga gcggcaatga atattccaca gctcacggcc
    32821 ctgtgcctgc gttatcaggg agtcttgctg gatgccagcg aagaagtggt tcatgttgcg
    32881 gtggtcgatg ccccctcaca tgagttgctg gacgcattgc atttcgctac caccaaacgt
    32941 attgagatca cctgctggac gcgccaacaa atggaaggtc acgccagtcg cacacaacag
    33001 acattgcccg tagctgttca ggagaagcat cagcccaaag cagagttgct gactcgaacg
    33061 ttacaatctg cgctggaaca acgcgcgtct gatattcata tcgaaccagc ggacaatgcc
    33121 taccgcatcc gcttgcgtat cgacggcgta ttgcatcctt taccggatgt ttcaccggat
    33181 gccggagtcg cattaacagc cagattaaaa gtgctgggaa acctggatat tgcggaacat
    33241 cgcctgccgc aggacggcca attcactgtc gaactggcag gaaacaccgt ctcatttcgt
    33301 attgcgacct taccatgtcg gggtggtgaa aaggtggtat taaggttgtt acagcaggtg
    33361 agccaagcac tggatgttaa cacgctggga atgcagccgt tacaactggc ggactttgct
    33421 catgccttgc aacaaccaca gggactggtg ctggtaactg gccctacagg cagcggcaaa
    33481 acggtcacgc tttatagtgc cctgcaaacg ctgaataccg ctgacattaa tatttgtagc
    33541 gtcgaagatc cggttgagat ccccatagcc ggactaaacc agacgcaaat ccatccgcgt
    33601 gccgggctca cctttcaggg tgttttgcgt gcgttattgc gccaggatcc tgacgtcatc
    33661 atgatcggag agatccgcga tggcgaaacg gcagaaattg ccattaaagc cgcgcaaacc
    33721 ggtcacctgg tgttgtccac gctacacacc aattccacca ctgaaacgct ggtacgttta
    33781 cagcaaatgg gggtcgcccg ctggatgcta tcatcggcgc ttacgctggt aatagcccag
    33841 cgtctggtac gtaaactttg cccacattgt cgccgacagc aaggggagcc catccatatt
    33901 ccagacactg tatggccgtc gccgctgccc cactggcagg cacccggttg tgtacattgc
    33961 taccacggtt tttatggtcg taccgccttg tttgaagtcc tgcccattac accagccata
    34021 cgccagctta tttccgctaa tactgaagtt gaatcgctgg aaacgcacgc ccgacaggcg
    34081 ggtatgcgta cgctttttga aaacggctgc ctggccgtgg agcaaggctt aaccaccttt
    34141 gaagagttaa tccgcgtact ggggatgccg catggcaagt aagcaactct ggcgctggca
    34201 tggcataacc ggcgacggca atgcgcaaga tgggatgcta tgggcagaga gccgtacttt
    34261 actgcttatg gcactacagc aacagatggt taccccacta agcctgaagc gaatcgccat
    34321 caattctgcg cagtggcgag gagataaaag cgcggaagtc attcatcaac tggcgacgct
    34381 acttaaagcc gggttaacgc tttctgaagg gctggctctg ctggcggaac agcatcccag
    34441 taaacaatgg caagcgttgc tgcaatcgct ggcgcacgat ctcgaacagg gcattgcttt
    34501 ttccaatgcc ttattaccct ggtcagaggc atttccgccg ctctatcagg cgatgatccg
    34561 cacgggtgaa ctgaccggta agctggatga atgctgcttt gaactggcgc gtcagcaaaa
    34621 atcccagcgt cagttgaccg acaaagtgaa atcagcgttg cattatccca tcatcatttt
    34681 agcgatggca atcatggtgg ttgtggcaat gctgcatttt gttctgccgg agtttgccgc
    34741 tatctataag accttcaaca ccccactacc ggcactaacg caggggatca tgacgctggc
    34801 agactttagt ggcgaatggg gctggctgct ggtgttgttc ggctttctgc tggcgatagc
    34861 caataagttg ctgatgcgcc gcccgacctg gcttatcgcg cggcagaaat tgctgttacg
    34921 catcccgatt atgggttcac tgatgagggg acaaaaactc acgcagatct ttacgattct
    34981 ggcgctgaca caaagtgcag gcattacttt tttacagggc gtagagagcg tcagagaaac
    35041 aatgcgctgc ccgtactggg tgcaacttct gacacaaatc cagcacgata tcagtaacgg
    35101 tcatcccatc tggctggcgc taaaaaatgc cggtgagttt agcccgctct gtttgcaatt
    35161 agtgagaaca ggagaggcat ccggctcgct ggacctcatg ttagacaacc tcgcccatca
    35221 tcatcgggat aacacaatgg cgctggcgga taacctcgca gccttactgg aaccggcgtt
    35281 gctgatcata acgggaggaa ttatcggtac gctggtggtg gcaatgtatc tgccaatttt
    35341 ccatttaggc gatgcgatga gtgggatggg ataacgctgg cgtgttgctc cacgccagcg
    35401 ttgggagatt acaggttgtt gaagatgcgg ttttcttgtt ccagcacacg aataaacgtg
    35461 gtgcgcttgg tcagctcttt caggcgtgaa gccccaacgt atgtacaagc tgaacgcagg
    35521 ccgcccaaaa tatcacgcgc ggtattttca accgggcctc gcagcggcag cttgacggtt
    35581 ttaccttctg ctgcgcggta ttccgcaacg ccgccaacgt gacgtttcat cgcagactcg
    35641 gagctcatgc cgtagaacag cataaatttc tcgccgttct cctcaacgat gcgaccgccg
    35701 ctctcttcgt ggcccgccag catgccgcca agcatgacga aatcggcacc gccgccaaag
    35761 gctttcgcca catcgcccgg cgtggtacag ccaccgtcgc tgacgatcat tccgcccaga
    35821 ccgtgcgccg catcggcaca ttcgattacc gcagaaagct gcggataacc gacgcctgtt
    35881 ttgacgcgag ttgtacaaac agaacctggg ccaatgccaa ctttaacgat atcagcaccg
    35941 gagaggataa gctcctcaca catttcacca gtcactacgt tgccagcaca aatggttttg
    36001 gtcggccacg cttcacgcgc tttcgcaacg aactgcacga agtgttcgga ataaccattc
    36061 gccacatcaa tacaaacgaa gtttaatgcc gggttcaggt cgagaatctg tttagttttt
    36121 tcgaaatccg catcagacgt gccggtagaa accatcacat gtttcagcac atcagcggaa
    36181 gaattgttga taaacgcttg ccactcttcg acagaaaagt gtttatgcac agcagtcaga
    36241 atatcaaaag aagccagcgc agaggccata gaaaatgtgc ctacggtgtc catatttgcg
    36301 gcgataatcg gcacgccgga ccagctctga cctgaatgtt tgaaggtgaa ttgacgttcc
    36361 agttcaacat cggaacggct tttaagagtg gagcgtttag ggcggatgag aacgtcttta
    36421 aaacctaact tcagatcttc ttcaatacgc atgtgcggat tcctggggtt aatggctata
    36481 agggtaagag cgcaactcca gtgacgctat catacgcact aatcaatgcg gcgcaagact
    36541 gcgaaattgt ctttttttac gctacaatcc cataaattta cctggtaaat agtgagcgaa
    36601 cgtttcatca atttcacttt cgcgcgctca tttttaacgt tttgataatc atgatgaatt
    36661 cccgggaata taaattatgg ggtatatagt tgccttaacg ggaggcattg gcagtggtaa
    36721 gagtaccgtt gccaatgcgt ttgctgatct cggaattaac gtcattgatg ccgatattat
    36781 tgcgcgtcag gtggttgaac caggtgcacc tgcgctacat gccattgctg atcactttgg
    36841 cgaaaacatg attgctgctg atggaacatt gcagcgccgg gccttgcgcg agcggatctt
    36901 cgccaacccg gaagagaaaa actggcttaa cgacctgctg catccgctga ttcagcaaga
    36961 gacgcaacac cagatccagc aagcaacttc cccctatgta ctgtgggttg tgccattgct
    37021 ggtagaaaac tcactgtata aaaaagcgaa tcgagtgctg gtggtggatg tcagcccaga
    37081 aacgcaactt aagcgcacca tgcagcgcga tgatgtaact cgcgaacatg tcgaacaaat
    37141 ccttgctgct caggcaacgc gcgaagcccg ccttgccgtg gcagatgacg tcattgataa
    37201 taacggcgca ccggatgcta tcgcatcgga tgttgcccgc ctgcacgcac actatttgca
    37261 gcttgcgtcg cagtttgtct cacaggaaaa accgtaatgc agacccaggt cctttttgaa
    37321 catccactaa atgaaaaaat gcgtacatgg ctgcgcattg agtttttgat tcagcaactc
    37381 accgttaatt tacccatcgt tgaccacgct ggcgcgctgc atttcttccg taatgtcagt
    37441 gaattactgg atgttttcga gcgcggcgaa gtccgcactg agctgttgaa agaacttaac
    37501 cggcagcaac gtaaactcca gacctggatt ggcgtgcctg gcgtggacca gagccgtatt
    37561 gaagcattaa ttcagcagtt aaaagcggcg gggagcgtat taatttccgc gccgcgtatc
    37621 gggcaatttc tgcgtgaaga tcgtttgatt gctctggtgc gtcagcgact gagcatccca
    37681 ggcggctgtt gcagctttga tttacctaca ttgcacattt ggctgcatct accccaggcg
    37741 cagcgcgaca gccaggtaga aacctggatt gccagcctga acccgctcac ccaggcactt
    37801 accatggtgc tggatttaat tcgccagtcg gcccccttcc gtaaacaaac cagcctgaat
    37861 ggtttttatc aggataacgg tggcgatgcc gacttgctgc gcctgaatct gtcgctcgat
    37921 tcacagcttt atccgcaaat ttccggacat aagagccgtt ttgccattcg ttttatgccg
    37981 ctggacactg aaaacggaca ggtaccggaa cgtctggatt tcgaactggc ctgttgctaa
    38041 ggagtaaaaa tgtcagaaac tattacggtg aattgcccaa cctgcgggaa aacggtggtg
    38101 tggggtgaaa tcagcccgtt tcggccattt tgctccaaac gttgtcagct gatcgacctc
    38161 ggagaatggg ctgctgaaga aaaacgaatc cccagcagtg gcgatctttc cgaaagcgat
    38221 gactggagcg aagagccaaa gcagtgacat ttgtctgatg cctcacgtag gcctgataag
    38281 acgcggacag cgtcgcatca ggcatcttgt gcaaatgtcg gatgcggcgt gaacgcctta
    38341 tccaacctac agacgcttaa gcttcgcaat taccggttca ttggctggcg gaaaatcatc
    38401 ggcattaaga ccgaccagcg acatccactc accgggttgc ccttctttac cccacggcac
    38461 cccttcccaa ctttcgacca gccagaacca cagagttata tgcctgtccg ggaattcata
    38521 ttccagtttt tcaaatagcg aaaaatgttg tggggtaatc ccgacctctt cctgaagttc
    38581 acgcaccacc gcctgttccg gcgtttcgcc catttcaatt ttaccgccgg gaaactccag
    38641 tttattcgcc atgtgcgcat ctgctgcgcg acgtgttata aagatttcat tgttctcgtt
    38701 gcgaataata cctaccgcaa tttgcagctt tttcattgtc ttaaacctat aaaaaaggcg
    38761 cagaatcctg cgccttttac ttcaactgtt agcttttatt gcaggcgacc atggcactgc
    38821 ttgtattttt taccagaacc gcacgggcaa ggatcgttac gtcctacttt gcgttcaccg
    38881 gtttgcgccg ccagtgcagc tgcggctgca gagtcgtcat cctgatggct aagctgctgc
    38941 atttgtgcta aacgctcggc ttccatacga cgctgttgtt ccagctcctc aacctcttca
    39001 ggcatacgta cctgaacttt gctcagcgta ctgataactt catatttcaa cgactccagc
    39061 atcgctgcaa acatggagaa cgattcacgt ttgtattcct gcttcggatc tttctgtgca
    39121 tagccacgca ggtggatacc ctgacgcaga tagtccatcg ctgccaggtg ctctttccac
    39181 agggagtcaa gcgtttgcag catgacgcct ttctcgaagt gacgcatcat ctcagcacca
    39241 accacttctt ctttacgctg atacacttcg atggactgcg ccagaatgcg ctcacgcagc
    39301 gtctcttcat gcagttctgg ttctttatcc agccactcgg caattggtaa atcgaggtcg
    39361 aaatcgttct tcagacgttc ctgcagcccc ggaatatccc acatttcttc cagcgactgt
    39421 ggcggaatgt aggcatcaat ggtggctttg aacacatctt cacgaatgct gttgatggtt
    39481 tcgctcacat cgctgacatc cagcagttcg ttacgctggg agtaaatggc gcgacgctga
    39541 tcgttagcca cgtcatcata ttccagcagt tgcttacgaa tgtcgaagtt acgactttca
    39601 actttacgct gggcgttggc aatcgcttta gtcacccacg ggtgttcaat ggcttcgcct
    39661 ggcttcatac ccagtttacg catcatgccg gatactcggt cggaagcaaa aatacgcatc
    39721 agcgcatctt ccatcgacag gtagaaacgg gaagaaccag catccccctg acgaccagaa
    39781 cgaccgcgca actggttatc gatacgacgg gattcgtgac gctcggtacc gatgatatgc
    39841 aggccacctg cttccagtac cgcatcgtga cgtacctgcc agtcggcttt aattttttca
    39901 atttgctctg cggtcggatt ttccagcgcg gcaacttctg cctgccagct acctccgagc
    39961 acaatatctg taccacgacc cgccatgttg gtcgcgatag tcaccgcagc cggataacct
    40021 gcctgagcaa caatcgccgc ttcgttggcg tggaatttgg cgttcaggac attgtgctta
    40081 ataccggctt tggtcagttc gtttgacacc agctccgatt tttcgatgga gatagtaccc
    40141 accagcaccg gctggccttt cgcagtacgt tctttgatat cttcaatgat cgcctgaatt
    40201 ttttccgctt cagtcatgta gaccaggtcc ggcagatctt tacgaatcat tggacggttg
    40261 gtcggaacaa cgacggtatc cagtttgtag atggagctaa attcgaaagc ttcggtatca
    40321 gcagtaccgg tcatccccgc cagtttttca tacagacgga agtagttctg gaaggtgatc
    40381 gaagccagcg tttggttttc gttctggatc tgcacacctt ctttcgcttc cacagcctgg
    40441 tgcagaccat cggaccagcg tcggccctgc atggtacgac cggtgtgttc gtcaacgatg
    40501 ataacttcac catctttaac gatgtagtcg acgtcacggg taaacagcgc atgagcgcgc
    40561 agcgccgccg ttacgtggtg catcagcatg atgttggccg gagagtacag agactcccct
    40621 tcatccatga tgccctcttt caccagcagt tcttcaatca gcaccagacc acgttcggtc
    40681 aggttcacct ggcgagattt ttcgtccacc gagaagtggc cttcgccctg gaaggtttcg
    40741 gagtcttctt tttcctgacg gatcaggtgc ggaataattt tattcacgcg tttatacatt
    40801 tccgagctgt cttctgccgg gccggaaatg atcagcggtg tacgcgcttc atcgatcagg
    40861 atggagtcca cttcgtccac cagcgcatag tgcagtttac gctgtacacg ttcttcaggg
    40921 ctgaacgcca tgttgtcgcg caggtagtca aagccgtatt cgttgttcgt accgtaagtg
    40981 atgtcagctg cgtaagcttc gcgctttgcc ggtgctggca tgcccggcag gttgatacca
    41041 acagtcaggc caaggaattc aaacagcgga cggttgtttt cggcgtcacg ttgcgccagg
    41101 tagtcgttga cggtaactac gtgcacgcct ttaccggtta gtgcgttcag gtaagcaggc
    41161 agcgttgcgg tcagggtttt accttcaccg gtacgcattt cggcgatgca gcgttcgtta
    41221 agaaccatac cgccgagtaa ctgaacgtcg aagtgacgca taccaaagac gcgcttactt
    41281 gcctcacgca ccacggcgaa agcttccggg atcagatttt ccagcacttc gcctttttcc
    41341 agacgcgcac gaaactctgc ggttttccct ttcagttctt catcggagag tttttccatc
    41401 tccggttcca tggcattgat gatgttgacc actttgcgca tccggcgcag ggtgcgatcg
    41461 ttacgactac cgaaaacttt agttaacaat ttgattagca taataaaatc ccaaacgccc
    41521 cgcgttacgg agttaataaa atgaagtaaa ggtttattgt tgttaggtga ggcgttgagg
    41581 gccagcacgg atgccttgcg cctggcttat ccagacgggc gtgctgaatt ttgcttgtgg
    41641 ggtaaaatgc gcataatcaa tgcgataacc cttttcagac ggcgtgcctt cctgggtcag
    41701 cagcgcgctg agcgtatcca gtaatgcaag atgttgcgcc tgaagaggca aagattcttc
    41761 agcaacgggc agtgtttgcg gtgccattgc gaaagaaaga tgacggatta ccgtgcgaat
    41821 agcatgttga tgccagtaat caacggaata gttcgaattc gggcggcgcg tgttcgcttc
    41881 cagcaaggcc aattgaccaa agttaacttt ggctgaaggc tcgtggttgc gggttgtcgc
    41941 ttttgcgggc gcgtttggtt cggcggcgtt gctgagcgca ggcaaaccta aactcgccgc
    42001 gaccatccct aataagagat gcggccagaa gtagcgttta ccaaactgtc gccagcgcgt
    42061 cagtattcca ctcacgttat tgccatcccg atcgaattca aacgtattta gcgcacaaat
    42121 cttatcatta aagcctgaag tccacagcac gaatgaagat aagttgtgcg taaaagtgct
    42181 taagaaaaca gcgttcgcac cgttttttat agctcttata aaattcaaaa aaaaacgact
    42241 ggttcacctg gccggagaga gtgccagatt tgccagtcga attttatacg acagtataaa
    42301 tgtcgttatg ccagtacagc tgaaggcgct ttgaaggcca acggcagttc tgcgtcgtcc
    42361 tggaaggtca catattccca ggcttcctgt ttcgccagga cagcctgcag cagtttgtta
    42421 ttcagtgcat gaccggattt ataagcggta aatgcaccaa taatattgtg accacacatg
    42481 aacaagtcac cgatcgcatc gagcattttg tgacgcacaa attcgtcttc aaaacgcagg
    42541 ccgtcttcgt tcagtacgcg ataatcgtca acaacgatgg cacaatcgaa gctgccgccc
    42601 aggcacaaac cacgggactg cagatattcg atatcacgca tgaaaccgaa cgtacgcgca
    42661 cggctgatct ggcgcataaa cgcatcagcg gagaagttca tcgcatagcg ctggttgctg
    42721 gaatcaatag ccggatggtt aaaatcgatg gtgaaatcca gcgaaaaacc attgtacggc
    42781 ttaaattcag cccacttatc gccatcttcg acacaaacag tctctttgat gcgaacaaat
    42841 tttttggcgc agttcaactc gtcgataccg gcgtcaagca gcaggtatac aaacggagcg
    42901 gcgctgccgt ccatgatcgg gatttccggc gcgttaactt cgataacaat gttatcgatg
    42961 cccaggcccg cgagagcagc attgaggtgc tctacggttg aaatccgtac atcatgctcg
    43021 ttgaccagac acgtacagag catggtatca cgcacagatt tggcatcggc cgggaaatct
    43081 accggtggat tcaagtcggt gcgacgatag atgaccccgg tgttggccgg cgcagggcgt
    43141 aacgtcaggg tgactttctt gccggtatgt aaaccgacac ccgtcgcctg aacgatacgt
    43201 ttaagtgtcc tttgtttgat catcgtatta tctcgccaaa ttacctatcc aaccgaagtg
    43261 tactatacat tcggcgggcc agtttagcac aaagagcctc gaaacccaaa ttccagtcaa
    43321 ttcttaatca gcttgcttac gtaggaatgc tgggatatcc agataatccg gctctttcgc
    43381 agtttgcggc gcattgtcat tcactacttt agcaaccggc ttctgctcct gggtcagcgg
    43441 agccatccca tgctgctggt agcgatccat cactggctgc tgaacctgct tattggtcac
    43501 cagagtgatt tcaggacgtt tgtccatgcc gatacctgtc gcaacaacgg ttacgcgcag
    43561 ctcgtcattc atatccgggt caagagaagt accgataacc acagtcgcgt tgtcggaagc
    43621 aaatgcacgg atggtgttac ctaccgtttc gaactcatcc agacgcaggt cgaagcccgc
    43681 cgtgatgtta accagcacgc cgcgcgcgcc agacaggtcg atatcttcca gcagcggaga
    43741 agagatagcc atttcagcag cttcttccgc acggtcttca ccgctcgcca cgccagaacc
    43801 catcattgcg tagcccattt cagacattac ggtgcgtacg tctgcaaagt ccacgttcat
    43861 caaacccgga cgagtaatca gttcggcgat accttgcaca gcgcctttca gtacatcgtt
    43921 cgctgcgcca aacgcatcca gcaaggagat accgcggccc agaactttca gcagtttgtc
    43981 gttcgggata gtgatcagag agtccacatg cttggacagt tcagtgatcc cctgctcggc
    44041 gaatgccata cgcttcttgc cttcaaagtt gaaaggctta gtgacgacag caacggtcag
    44101 gatacccaaa tcttttgcca cttcagcgac gactggtgct gcacctgtac cggtaccacc
    44161 acccataccc gcagcaataa agaccatgtc tgcaccttcc agcgccgcac gcaatgcatc
    44221 gcgatcctca tcagccgcat tgcggccaac ttctggatta gcgccagcgc ccagtccttt
    44281 ggtgataccg ctaccgattt gaatcgtctg tccaaccgct gttttacgca gcgcttgtgc
    44341 atcggtattt accgcgaaga attcaacacc ttcaatacgc tcgcgcacca tgtgttcaac
    44401 agcattaccg ccgccgccgc cgacgccgat gactttaatc accgcgtcat tggtaagttc
    44461 cattggttca aacatagttt ctctccgatt tgtgcctgtc gcctgaggcc gtaatcatcg
    44521 tcggcctcat aaaaattaaa actcttttcg cagccaacta ttgagtcgct tgatccacga
    44581 gccaactgat gctgtaacac gtttttctac ttcagcttca ccgttaagat gtgactcttt
    44641 cccatagtga agcaatccca ccgccgtcga ataatacggc tcctgagcat aatccgttaa
    44701 accggtaatg ttcagcggcg cgccgatacg cacttgcgta tgaaacacgc gctgagcaca
    44761 ggctgcaaga ccttcgatct gcgctgcgcc accggttaat acaatgcctg ccgccaggtg
    44821 atgtttaacc ccttgttggc gaagcttttc ctgcaactgc aatatctctt cgttgaccag
    44881 gttgagcaac tcggtatagc gcggctcgat cacctctgcc agggtctgac gttgcagact
    44941 ccgcggcgga cgaccgccta cgctcggcac ttccacgctc tcatcttttc caacgatgga
    45001 acccagcgca caaccgtggc gaactttaat cgcttcggcg tcgcttggcg gcgtgccaaa
    45061 ggcgtaagcg atatcactgg tcacgacatt gccagcataa ggaattacct tagtgtggcg
    45121 caatgcccca ccggtataaa cggcgatatc cattgtacca ccaccgatat cgacgacgca
    45181 gacacccagt tcacgttcat cttccgtcaa taccgaataa cttgatgcca gtccggcaaa
    45241 tatcagttgg tcaactttca gcccacaacg ttcaaccgct ttgacgatgt ttttcgccat
    45301 atcgttgtga catgtgatca ggtgcacttt tgcctgcatc cgcacacccg aaagtcctac
    45361 cggattcttg atcccttcct gatagtcaat cgcatactct tgcgggatca catgcagcac
    45421 acgatgctca tcgcgcacac gcaccgattt cgcggtatgg acgacgtttt ccacatcttc
    45481 ttgcgtcact tcttcttcag aaataggcac cataccaatt tcattctggc agctgatgtg
    45541 cttaccagaa agcgccagat ataccgaaga gatctgacaa tctgccatca attctgcctg
    45601 gtcaatggcg cgttgtacgc acttgaccac ggattcgagg tcgttcaccc cgcctttatc
    45661 cataccacgt gacgggcagc tgcccacgcc aatgatattg accataccgt cgggcagaac
    45721 ttcccctact aaagcggcaa ccttcgcggt accaatttcc agtcctacta ccagttttct
    45781 gtccgtcgcc ttgatcattg ttgttctgcc tgtgcctgat tttgttgctg agtagattcc
    45841 tctggcggca agggcgccca gcctactgcc gctccagagt cataacgcaa atcaacgtag
    45901 ctaatccgtt tgccatcggt ttgcgcctgc tgctgtaaaa ccggataaag ttctacaaag
    45961 cgagccaaac gtttcatcgt atcgccccgg ccaagattga gcttaatatc gttattcagc
    46021 gtcaactgcc aggaacgccg cgcggtcatc gccgcttcct tcagagtaaa tctgtccttt
    46081 gccagcatct gccccatttc gcgatagccc tgcaacactt cattagcgct gccttccggg
    46141 ccatacagca ttggaagcac ctgcttgctg gtgcgatctg gcggcacgct gaaggtgttt
    46201 ccttccgcgt ctaccatgtg ttgatcattc caccgcgcaa tcggcacata ttcaaccaga
    46261 tgaatcttca attcatcagg ccactgcttt ctgacgctca cctgcttaat ccacggcagg
    46321 cgttgttcta tttgcgtctg gatgatgttg acatcctggg tcataaaggt acccggctca
    46381 cccaatgcca ggatcgactg ccggatatcg tcattacgcg tgtaatggcg ttcaccggtc
    46441 aacaccagct ttgagagcgg caggcgttgc gcatcttcca tccagctcaa cacgacccag
    46501 ccgctcacca acactgtcgt taaaacggtc agcaggaaaa ggatccccgc cagacgcgtt
    46561 ccattattgc ggcgagaaga aacctcttct tcgctgtttc gcgtgttcag agcagcctgc
    46621 gacatattag tccgccagtt ccagaattcg tactaccaac tgcgagaagc tcatacctgc
    46681 ctgacgtgcc gccatcggca ccaggctgtg gctggtcata cccggtgagg tattggcttc
    46741 cagcagataa aactgtccat cactgtccag cataacgtca atacgtcccc atcctttgca
    46801 acctaacgtc gtccatgctt tcagcactaa tgcctgcaaa ttggcctctt gtgacgcttt
    46861 cagacctgcg gggcagaaat actgtgtctc atcagagaga tacttcgcct cataatcata
    46921 gaaggttccg gacggttgaa tacgtattga cggtaaaatt tcttcaccga gtatcgcaac
    46981 cgtgaactcc ggcccactta gccatttttc aatcaatact tcttcatcgt gctgaaatgc
    47041 caatcttaat gcatcttgta gagcattttc tgctactact tttgacattc ccacactgga
    47101 accttcgcgg ctcggcttaa cgataaccgg caaacccaga gcagaaattt ctgctaactg
    47161 cttatcgctc aggccttttt caaactctgc gcgggttaac gctacccacg gcgcgaccgg
    47221 taaaccggca ccttgccaca gaagtttgct gcgtagttta tctattgaaa gcgcagatgc
    47281 catcactccg ctgccggtat aaggcaagcc catcagctcg agcatgccct gcagcgtacc
    47341 atcttcaccg ccgcgaccgt gtagcgcgat aaacactttc tgaaaaccca tcgacttcag
    47401 ttgcgtcacg tcgacttctt tcgggtcgac aggatacgca tcaataccgc cttcacgcag
    47461 cccggctaac accgctgcgc cagaattcag agaaacttcc cgctcagcgg aagtcccgcc
    47521 caacaggacc gcgattttat cagtcatgtt gttcttcctc cggagtttgc ggcttcagtt
    47581 tgatttcagc taaagaacgg gcaatttttc cgatattacc agccccctga acgagaatca
    47641 ggtcgttacc ggttaatacc ggtgccagca tctcggctac ctgcgcaggg tccggcacca
    47701 gaatgggatc aattttccca cgtccacgaa ttgtgcgaca cagcgaacgg ctgtccgctc
    47761 ccggaattgg cgcttcgcca gccggataca cttccagcat caacagggta tcaacctgcg
    47821 tcagcacatt ggcgaaatca tcatacaggt cgcgcgtacg ggtaaaacgg tgcggctgaa
    47881 acagcattac caggttttta tccggccagc ctgcgcgcgc cgctttaatg gtggcgtcaa
    47941 cttccgtcgg gtggtggccg tagtcatcga ccagcattgc cgtaccgctt ttaccattca
    48001 ctggctccag cgggaattca ccgaggaaat caaaacggcg accagtcccc tggaagctct
    48061 ccagcgcacg caaaatagcc tcgtcgtcaa ttccctcttc cgtagcaacc gcaaccgcag
    48121 ctgcggcgtt cagcgcgtta tgacggcctg gcgcattcag ggtgacgcgc atcggctctt
    48181 tgtcctgacg cagcagcgta aagtgcccct gcgggccaat ctgctgataa tcttctacac
    48241 gcacgtcggc atcttcgctg aagccgtaag ttgtggtctg acgtcccaca cgcggtaaca
    48301 attcgcggat caccggatca tcaacacaca tcaccgcacg accgtaaaaa ggcaggttgt
    48361 gcagaaaatt aataaaggtc tgttttaaat tctcaaagtc gccctggtag gtatccatgt
    48421 ggtcggcttc gatattggtg acaatcgcca ccatcggttg cagatgcagg aacgatgcat
    48481 cactctcatc tgcttcggca atcaggtacc gaccatgccc caaacgcgca tgaacccccg
    48541 ccgcttttac cagcccgccg ttaacgaagg ttgggtcgag ccccgcttct gcgtagatgc
    48601 tggaaaccat cgcggtagtt gtcgttttgc cgtgcgttcc tgcaatggcg atgccatgac
    48661 gaaaacgcat taactcagcc agcatttcgg cacgacggat caccggaata cgcgcttcat
    48721 gagctgcgac aatttccggg ttgtcggcag aaatggcgct ggaaacaacg accacgctgg
    48781 catcacgtac gttttccggg cgatggttga aataaatcgt cgcacccaga ttcagtaact
    48841 gctgcgtgac cggatttggc gctaaatcgg aaccactgat ctgataacct tcattggcca
    48901 gaacttcggc aataccgccc ataccggcac caccaatgcc gacaaaatgt atgtgccgaa
    48961 cgcgacgcat ttcgggcacg atggaacgca gttttgccaa ttgttgtgta ttcattcttt
    49021 acgccattaa cttcttaaat tcatacgatg caaaaggcat cgctacaatt acgcccgggc
    49081 agcccggctc acttcatttg ccactcgctc ggtggcatcc ggaatggatg cagcgcgggc
    49141 gcgttctgcc atggttaata aggtttctcg cgaccacccg gccagggtgt tggcgacagc
    49201 atccacgcta agctgtggct gctcgataat tttggctgcg cccgcttttt ccagcggtag
    49261 cgcattccag tattgctggc ggtctttatg ttgaaacggc acaaacaacg ccggtagtcc
    49321 tgccgcggcg atttcactca cagttaaagc accggagcgg caaacgacga catccgccca
    49381 cgcatacgcc gccgccatat catcaataaa ttcagtcact ttatgctgcg gttgccccgc
    49441 ttcggcatac gcctgttcaa cggattgttg cgaacctttg ccgctctgat gccagatagt
    49501 gactgaatca cccagtttcg cagcaacctg cggcattgtc tggttaagaa tgcgtgcgcc
    49561 ctgggaacca cccactacca acacacgaac cggaccttca cgtccagcca aacgttgctg
    49621 cggcaacggc agcgccagca catcggtacg caccgggtta cccaccactt ccgcattagg
    49681 gaaagcgcct ggaaacgcct gcatcacttt ggtagcaatc ttcgccagcc atttattggt
    49741 taagcccgca ataccgtttt gttcatgaag tacaaccgga atgcctaacg accacgcggc
    49801 cagaccacct ggacctgata catagccgcc cataccgagt accacgtcag gtttgtacgc
    49861 tttcataatc gcccgcgcct gacgccaggc gttgaagata cgcagcggcg cagctatcag
    49921 tgcttttata ccttttccac gcaaaccaga gatacgaatg aaatcaattt cgatgccatg
    49981 ttttggcact aagtccgctt ccatacggtc ggcagtcccc agccagcgaa cttgccaacc
    50041 ctgagccatt agatagtgcg caaccgccag tcccgggaat acatgtccac cggttccgcc
    50101 tgccatcacc attaatcgct ttccttgacc actcatcgtg aacctcgtac aaacgcctgc
    50161 gctttctcca gacgcgtttc ataatcaata cgcaacagca tcatgatggc tgtcgacata
    50221 atcagtaagc tcgaaccacc gtaactgatt agcggcaatg tcagaccttt ggtcggtaac
    50281 atccccgccg ccgcgcctac gttaaccagc gcctggaagc taaaccagat gccaatagaa
    50341 caggcgagaa aaccggaaaa acggtggtca atttctaatg ctttgcggcc aatcgacatc
    50401 gcacgaaaag cgacgaagaa taccattaaa agtgctagta ccacaccgac ataacctaat
    50461 tcttcgccga taatggcgaa aataaagtca gtgtgcgctt ccggcagata ctccagtttt
    50521 tgtaccgaat tacctaaacc ttgcccccaa agttcgccgc gaccaaacgc catcagcgat
    50581 tgcgttaact gatagccgct gccaaaggga tcttcccacg ggttccagaa tgcggtaaca
    50641 cggcggatac ggtacggttc ggcgaggatc agcaacacaa ccgctgaaat gcccataccg
    50701 ataatggcaa tgaactgcca caatttcgct cccgccagga acaacatcgc cagcgtagtc
    50761 acaaacaaca ccaccaccgt accaaggtct ggctgtgcca gcagtaacac tgccaacacc
    50821 agaatcacgc ccatcggttt caggaagccg cgcaggttat tacgtacttc gtcgccttta
    50881 cgcaccagat agttggcgat atagcaaaac aacgacagtt ttgtcagctc cgcaggctgg
    50941 atacgcagca aaccgagatc gatccaacgc gatgcccctt taacagcgct acctactacc
    51001 agcacgatca tcagcaggat gatagatccg agcagcatcg tggcactgta gcgttgccag
    51061 aactccatcg gcagacgcag cgtaatgatc gccagaataa acgccaaaat cagatagaca
    51121 ccatcacgct tcgcgaagaa gaacggatcg ttggttaagc gttgccctat gggcattgac
    51181 gccgaggtca ccatgataaa gccaatcgcc gcgaggccga aggtcagcca cagtaaggtg
    51241 cgatcgtaca tgatcaggct gtcggtatct ttttcccgcg agcccatcac ccagcccttt
    51301 agcgccgtgg agatccagac caagatactg aatcctggca ggcgcggcat tttcaggcga
    51361 gggagagata aacgcatcaa cctaactcct tcgccagacg ggcaaactca ttgcctcgtt
    51421 gttcaaagtt cttgaactga tcaaggctgg cacaggccgg ggagagcaga accatatcgc
    51481 ccggctgaac acgcggagcc agcaagcgca tagcctgttc catagtttcg gtttgttctg
    51541 ccacttccgg gcgtagcgcc gccagctgcg cgccgtcacg accgaaacaa tacagacgta
    51601 cgttatcgcc attcaggtaa cgcgccagtg ggctaaagtc cgccgattta ccatcgccgc
    51661 ccagcaacaa atgcagcgtg ccgtctacgt gcaggccatt cagcgccgct tccgtactgc
    51721 cgacgttggt cgcttttgaa tcgttaatcc agcgcacacc gttatgctcc agcaccactt
    51781 caaagcgatg cggcagaccg gtgaaggtgg ttaacgcttt caggctgctg gcgcgcggta
    51841 accctgcggc atctgccagc gccagcgccg ccagcgcatt ggtgtagtta tgctgcccgg
    51901 aaagtttcat ctctttcaca ttcagcactt tctcgccttt aacccgcagc caggtttcgc
    51961 cctgctgatg attcaggtga tagtcaccca tgttgacgcc aaagctgacg cagcgttcat
    52021 ccgcaccgcg aatcggcatt gttaaggcat catcagcatt aaccacgcaa actttcgcgt
    52081 tttcgtaaat gcgcagtttt gctgcacgat actgttgtaa accaaacggg tagcgatcca
    52141 tatgatcttc agtcacgttc agaatggtcg ctgctaccgc ctgtaagctg gaggtggttt
    52201 ccagctggaa gctcgacagt tccagcacgt acagttcaca ctcatcatcc agtagcatca
    52261 acgcaggcag gccaatattg ccacccacac caacgttaac ccccgccgct ttcgccattt
    52321 cacccactag cgtggtgacc gtgcttttgc cgttagaacc ggtaatcgcc acaatcggtg
    52381 cttgtgcttc gcgacaaaac agctcgatat cgccaacgat ttcgattccg gcatcagcgg
    52441 cagcgcttaa ggatggatgc gccagtgcaa taccgggact ggcgacaatc agatctgccg
    52501 ccatcagcca ttcatcattc agactgcccg tgtggcgttc tacggcttcg ggtaatttat
    52561 ccaggctagg cggtgtcata cgcgtatcca taacgcgcgg cgtcacaccg cgagcgagga
    52621 aaaagtccac gcaggaaagc ccggtgaggc ccaggccgat aatgacaaca tttttacctt
    52681 ggtaatcagc catgattaac gtaccttcag cgttgccaga ccaatcagga ccagcatcag
    52741 cgaaataatc cagaaacgca caatgacgcg cggttccggc cagcctttca gttcatagtg
    52801 gtgatgaatc ggtgccatgc ggaaaatacg ttgtccgcgc agtttaaagg agccgacctg
    52861 caggatgaca gaaagcgttt ctaccacgaa cacgcccccc ataatcacca gcaggaattc
    52921 ctgacgtagc agtacggcga taatgcctaa cgcaccacct aacgccagcg aaccgacatc
    52981 gcccataaag acctgcgccg gataggtgtt aaaccacagg aagcccagtc ctgccccgac
    53041 tatcgcggta cagacaataa ccagttcccc ggcgtgtcgc agatacggta tgtgtaaata
    53101 gctggcaaag ttcatgttgc cggtcgccca cgccaccagc gcaaaaccac cggcgacaaa
    53161 tacggtcggc ataattgcca gaccgtcaag accatcggtc aggtttaccg cgttgccagt
    53221 acccacaatg acgaagtaag ccagcagaat gtagaacagc cccagttgcg gcatcacatc
    53281 tttaaagaat gggaccacca gctgcgttgc gggcgtgtct ttgccggcaa ggtacaaagc
    53341 gaaagcaaca cccagcgcaa tgaccgacat ccagaaatat ttccaacgag cgatcaaccc
    53401 tttggtatct ttacgcacca ctttgcgata atcatcaaca aagccaataa caccgtaacc
    53461 taccagcacc accaacacgc accagacgta cggattggac gggtaagccc acagcagcac
    53521 ggagatcaca atcgctgtca ggatcataat cccgcccatg gtcggcgtac cgcgcttgct
    53581 gaagtgtgat tcaggaccgt cgttacgcac tacctgacca aaggaaagtt tttgcaaatg
    53641 agcaatcatg cgcgggccca tccacaatga gatgaacagc gcggtcagca ggctgacgat
    53701 ggcgcgaaac gtcagatagg aaaagacgtt aaagccggaa taatatttga ccaaatgttc
    53761 ggccagccaa actaacatgt cccattctcc tgtaaagcgc gtactacctc ttccatggca
    53821 gcactacgtg aacccttaac taaaatcgta attacctgtt gctcagcaat cagtgattta
    53881 agacgcgtaa ttaacgcagt tttatcagca aaatgttcgc caacgccgct ggcggtgctg
    53941 atagcatggc tttgtttacc catgcttaac acgcggtcaa taccagcagc ttttgccgcc
    54001 tcgcccacct gtacatggca ggcttcgctt tcagcgccca gttccgccat atcgcccacc
    54061 accagcacgc ggtagcccgg catttcagcc agtacctgaa cagctgcggt cattgaaccg
    54121 acattggcgt tgtaggagtc gtcgagcagc aactggtttt ctgccagttg gatggggaac
    54181 agacggccag gaacagcttt cagatttgcc agccccgctt tgatagcatc aagcgttgcg
    54241 cccacggaca tggagagcgc agcggctgcc agcgcattcg caatattgtg acgccccggc
    54301 aacggcagca gaacatcgac gctaccggtt ggggtttgta gggtaaattc cataccgtgc
    54361 gaggtcacat ggatattggt ggcggtgaaa tcgctgttgg cggcattggg tgagaaacgc
    54421 cacactttgc gtgagccaat tacgctctgc cagttcagcc agtcgttgtt gtcggcgttc
    54481 ataatggcga taccgttttc cggcaggccg ctaaagattt cacctttcgc tttcgcgaca
    54541 cccgcaagcg agccaaaacc ttccagatgc gccgctgcca ggttgttgac cagcgccgct
    54601 tccgggcgag tcagactcac agtccaggca atctcgccct gatggttcgc gccaagttca
    54661 ataactgcgt aatcgtattc cggcgttaag cgcaacagcg tcatcggtac accgatgtcg
    54721 ttgttgagat tgcctgccgt ataaagcgtg ttgccgcact ggcttaaaat cgccgccgtc
    54781 atctctttaa cggaggtttt gccggaggac cccgtcagag caaccacgcg cgccggaact
    54841 tgctggcgaa cccatgcagc cagttcacca aacgccagac gcgtatcctt gacgattaac
    54901 tgcggcaggt cgatgtccag cggacggcta accagtagtg cgcctgcgcc gccagctttc
    54961 gcctggtcgg caaaatcgtg ggcatcaaaa cgttcacctt tcagggcaac aaacaggcag
    55021 cccggcgtca gttttcgggt atcagtggtt acagcatcaa gggtgatatc tgcaccttgc
    55081 agttcaccgt tgagaatatc ggtaagttgg ctaagggtta cgctaatcat gcaatccccc
    55141 ccagcagacg cgccaccgtg acgcgatcgg agtagtccag acgctgattg ccaacaatct
    55201 ggtaatcctc atggcctttg cccgcgacca gtaccacatc attctcttta gcctgcataa
    55261 cggcgcaagt caccgcttca gcacggcctt ccatcacttt ggcatgtccg gcatctaaca
    55321 ttcccgccag aatatcgttg atgatggcac gcggttcttc ggtacgcggg ttatcgtccg
    55381 tcaccaccgc cacgtcagca aactcttcgg caattgcgcc catcagtgga cgcttacctt
    55441 tatcgcgatc gccaccacag ccaaagacgc accacagctt gcccgcacag tgcaggcgcg
    55501 ccgcctgtaa ggctttttcc agtgcatccg gcgtatgcgc gtaatccacc accaccgtcg
    55561 gtttgcctgg cgcagtgaac acttccatac gtccgcaaac cggttgcaga cgcgcggcgg
    55621 ttttcagcag atcagccagt ggatagccca gcgccagcag tgttgccagc gcgagcaaca
    55681 ggttgctgac gttaaaagcg cccatcagat ggctttcaat ttcgccatcg ccccagcttg
    55741 aactaaagcg aatggtcgcg ccgctgtcgt gatagttcac ttcggtcgct ttcaaccagc
    55801 gtccgtgaca gttcggattg atatgatctt ccattgatac cgcaaccgcg tccggcagtt
    55861 ttgccagcca gcggcggccc acttcatcgt cggcgttaat aatcgcctga ccgcaatgat
    55921 gttcagaata aagcagccat ttcgcagctt cgtagtgttc catatcacca tgataatcaa
    55981 ggtgatcgcg gcttaagttg gtaaagaccg acgccggaaa tttcaatgcc gccacacggt
    56041 gctgtaccag cccgtgggag gaaacttcca ttgcgcaaaa agtcgcgccc tgatccacca
    56101 gccccgccag ctcatgctga acatcaactg ccgaaccggt agtattttct gtcgggatca
    56161 ctttccccag cagaccatta ccaacggtgc ccattaccgc gctggtttcg ccaagcagtt
    56221 ggctccactg cgccagaagc tgggtcgtcg tggttttgcc gttggtgccc gttacgccta
    56281 cgagacgtaa attgtcagag ggttcatggt aaaagcggcc cgccagtgca gataaacgct
    56341 cgttgagctg gctgagatag atgaccggta cgccgtgcat ttcacggatt tcaccgtcgg
    56401 tcgcctcatc tttcgcctct gcaataatgg cagccacacc ttgcgctatc gcctgcggga
    56461 tatatcgacg cccgtccgcc tgatgaccta ctacagctac aaagagatcg cccgccgcag
    56521 ccacacggct gtcgagtgtc atctctcgca gtgctcgcga aggtgcgtct ggcacccacg
    56581 gagcaagaag gtcgcgcaaa ttacgatctg ccacctgtcc cctcgccttg attaatcaca
    56641 aattcatttt tatcgcccgt tgtcagcgca tccggctcga tgttcatggt acgcaatacg
    56701 ccgcccatga tggcaccaaa gaccggcgcg gaaacggcgc cgccgtagta tttacccgcc
    56761 tgcggatcgt tgataacaac aaccagcgcg aagcgcggct gactcgcagg cgcaacgcct
    56821 gcggtataag caatatattt attgatgtag cgaccgtccg gcccgacctt tttcgcggta
    56881 ccggttttaa tggcgatacg atagccttta atcgccgcct tcacgccgcc gccgcctggt
    56941 agcgccacgc tttccatcat atgcaccacg gtgcgaacaa tggattccgg gaagacacgt
    57001 tcaccgggaa ccggggggtc aactttggta atcgacagtg ggcgataaat gccgtagctg
    57061 ccgatagttg cgtagactcg cgctaactgt aacggtgtta ccattagccc gtagccgaaa
    57121 gagaaggtgg ccctctctat gtcagaccac cgttgttttt gaggatataa gccactgcgt
    57181 tctccgacca accccaaatt ggtcgctttt cccagtccaa aacgtgagta agtatctact
    57241 aacgctgagg acggcatcgc taacgccagc ttggaaacac cgacgttact cgacttctgt
    57301 aataccccgg tcagggttaa ttcgctgtag cgtgccacgt ctttgatttc gtggccgtta
    57361 attcgataag gaatggtatt gagtaccgag ttttcccgca ccacgccacg ttgcaacgcg
    57421 gtcatcacca ccatcggttt aaccgttgag cccggttcaa acacgtcggt gatggtacgg
    57481 ttacgcatcg cctctttcgg cgtgccgctc agattgttag ggttgtatga cgggctgtta
    57541 gccatcgcca gcacttcacc tgtgttgaca tccaccagca cggcgctacc agattcagcc
    57601 ttgttaaagg ccaccgcgtt gttcagttcg cgataaacca gcgcctgcag gcgttcatca
    57661 atactcagcg ccaggttgtg cgctgcctgg ctgtcagtag aagaaatatc ttcaattacg
    57721 cgaccatagc ggtctttacg cacaatgcgc tcacccggct gcccggtaag ccatttatcg
    57781 aaactcttct caacgccctc aatcccttga ctatcgacgt tagtaaagcc gatgaggtga
    57841 gcagtcactt cgccggacgg atagtaacgg cgagactctt cacgcagatg aatccccggc
    57901 agtttcagtt ttttgatgta gtccgccatg tcagagttta cctgacgcgc cagataaata
    57961 aagcgccctt tcgggttggc gttaatgcgg gcagaaagct gatccagcgg aatattgagc
    58021 gcgttagcca gcgccttcca gcggtcaccg acgctgatac cgccagcgtc atgcacttct
    58081 ttcgggtcag cccaaatcgc ttttaccggc acgctcaccg ctaacgggcg accagaacgg
    58141 tcagtaatca tgccgcggga ggtggaaact tgctgaacgc gaagagaacg catgtcgccc
    58201 tctttcacca gcatatccgg ggagataact tgtaaccacg ctacgcgtcc gagcagaaaa
    58261 gccagcgcca ggagaataca gccgcataac aacgcaaaac gccaactgat aaagttggca
    58321 tgttcttcct gacgttttgg tttctgcgtt ttcgccgctg ctttcatgcg tcgcgtttat
    58381 ccttattttt gcactacgat attttcttgt gacggatcaa catgctgcat ttgcagcttt
    58441 tccgtggcga tcctttccac ccggctatgg tcgccgagcg cattctcttc aaggatcagg
    58501 ttgcgccatt caatgtctaa agcatctcgc tccagcacca gttgttcgcg ctgagcggtc
    58561 agtaaacggg tatggtgcgc cgtggttacc acggtcaccg ccgtcaaaat aatgcaaatg
    58621 aacaggcaga gtggcagctt cccaaatcgc aaaagatcgt caccgataac accaggcaat
    58681 gcatggcgct cgtggcttcc catcgatcct ttaactttgc ttagagcttc tgtcactctg
    58741 ctgatcatgc gttcgtcctc tctgcaatac gcagaactga actacgggca cgagggttct
    58801 cagccacctc ttcttcgccc ggcattaact tgcctagtgc tcgcagctga cggccaccca
    58861 gttttttgag ctgctcttca gtcatcggta accctgccgg aacttgcgga ccgcggctgt
    58921 tttcacgcat aaaacgtttc acaatacggt cttccagcga gtggaagctg atgatcgaaa
    58981 gccgcccacc cggggccagc acgttgagcg agctttttag cgcctgctct atctcctcca
    59041 gttcactgtt tacccaaatg cgcaccgcct ggaaggtacg ggtcgcggga tgtttaaact
    59101 tatctttcac cggcgttgca gcagccacga cttccgccag ttctttggtg cgggtcatcg
    59161 gctgttcgcg gttacgctcg acaatagcgc gggcaatgcg tttggcaaaa cgctcttcac
    59221 cataggtttt caatacccag gcgatatcgg cttcttctgc ggtttgtagc cattcagcgg
    59281 ctgactgccc acgggttggg tccatacgca tgtccagcgg accatcgcgc ataaaggaaa
    59341 agccgcgttc agcatcatca agttgcggtg aagagacgcc aagatcgagg agaatgccgt
    59401 cgatcttgcc aataagatcg cgctcggcaa cgtattcgcc cagcgcggag aaaggtccgt
    59461 ggatgatgga gaagcgcgga tcatcaatag tcttcgcaac ggcgatagcc tgcgggtcgc
    59521 gatcgatcgc cagcaaacgc ccctcttcgc caagctgcga gaggatcaga cgtgagtgac
    59581 caccgcgacc aaaagtccca tcaatgtaga tgccatcagg acggatattg aggccattaa
    59641 cggcttcatc cagcagcacc gtagtatgtt tatagttttc catcatttta tagagacaag
    59701 tcctgcagtc gctccgataa gtctccggta gccaactgct ctgcgtcgat atcttccttg
    59761 acctgttgat gccaggttgt ttcatcccac agctcaaact tgttgaactg tccaaccagc
    59821 atcacttctt ttgtcagccc ggcatgttgc cgcagtactg gcgcgattaa caatcgacct
    59881 gcgccatcca tctgacattc gctggcatga cctaacagta ggcgctgcac acggcgctca
    59941 accgggttca tgctcgacag acgcgataat ttttgctcga taatttccca ttcaggcagg
    60001 gggtaaagca gcaggcacgg gtgatgaatg tcaatggcgc aaaccatttg accggcagcg
    60061 ttctcaagca gctgttcccg ataacgggta ggcactgata agcgcccttt gctgtcgaga
    60121 ttgactaacg ttgctccccg gaacatgcca gcctcacccc ttattcccac tttgccccac
    60181 aatttcccac tgaaaggagt ttacggagcg gaggaaaagc ttgtcaagcc agtcacaagg
    60241 cataccgagg taaaaaaccc gatgtttacg gctaatatca gcgttgttta ttttacgtcc
    60301 aacgtgcgaa gcaaattaac gttatgaata gctgtaagaa aaaatccctc atcgtcatca
    60361 ctgttttaaa atgactcact attttccaca gcaaatataa agtgtcagtt tgcgacgcga
    60421 gcggcatttt aggacatatc ttccccggtt aacagtcctt gttacgtctg tgtggcgctt
    60481 gatgccagcg ggtttgcgca aaagcaaaga aaagtgtttg ttaaatcggc aattcatcgc
    60541 cgcatgtaac aaaataatgc aaaaacgcgc tgtcgatatc acttaattat tgtgaatttc
    60601 agctggtgag ttaattaacg ggcgggttta aaaccattta tcaataatag tcaggaatag
    60661 tcttatttac tttaaggata ttgat
//
LOCUS       DAETPZ010000014        59523 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.014, whole genome shotgun sequence.
ACCESSION   DAETPZ010000014 DAETPZ010000000
VERSION     DAETPZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.014"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="K0W59_000997"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="K0W59_000997"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HBI3022135.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="K0W59_000998"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="K0W59_000998"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBI3022136.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="K0W59_000999"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="K0W59_000999"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBI3022137.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="K0W59_001000"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="K0W59_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HBI3022138.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="K0W59_001001"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="K0W59_001001"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HBI3022139.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="K0W59_001002"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="K0W59_001002"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBI3022140.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="K0W59_001003"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="K0W59_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HBI3022141.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWCGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="K0W59_001004"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="K0W59_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBI3022142.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEMAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="K0W59_001005"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="K0W59_001005"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBI3022143.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="K0W59_001006"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="K0W59_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HBI3022144.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="K0W59_001007"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="K0W59_001007"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HBI3022145.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="K0W59_001008"
     CDS             complement(15665..16702)
                     /locus_tag="K0W59_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBI3022146.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17034..17522
                     /gene="yhhY"
                     /locus_tag="K0W59_001009"
     CDS             17034..17522
                     /gene="yhhY"
                     /locus_tag="K0W59_001009"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBI3022147.1"
                     /translation="MSEIVIRHAETRDYETIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17581..19303)
                     /gene="ggt"
                     /locus_tag="K0W59_001010"
                     /pseudo
     CDS             complement(17581..19303)
                     /gene="ggt"
                     /locus_tag="K0W59_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            19423..19863
                     /locus_tag="K0W59_001011"
     CDS             19423..19863
                     /locus_tag="K0W59_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HBI3022148.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19850..20593)
                     /gene="ugpQ"
                     /locus_tag="K0W59_001012"
     CDS             complement(19850..20593)
                     /gene="ugpQ"
                     /locus_tag="K0W59_001012"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBI3022149.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20590..21580)
                     /locus_tag="K0W59_001013"
                     /pseudo
     CDS             complement(20590..21580)
                     /locus_tag="K0W59_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21582..22427)
                     /gene="ugpE"
                     /locus_tag="K0W59_001014"
     CDS             complement(21582..22427)
                     /gene="ugpE"
                     /locus_tag="K0W59_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HBI3022150.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22424..23311)
                     /gene="ugpA"
                     /locus_tag="K0W59_001015"
     CDS             complement(22424..23311)
                     /gene="ugpA"
                     /locus_tag="K0W59_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HBI3022151.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23409..24725)
                     /gene="ugpB"
                     /locus_tag="K0W59_001016"
     CDS             complement(23409..24725)
                     /gene="ugpB"
                     /locus_tag="K0W59_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HBI3022152.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25122..25835)
                     /gene="livF"
                     /locus_tag="K0W59_001017"
     CDS             complement(25122..25835)
                     /gene="livF"
                     /locus_tag="K0W59_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HBI3022153.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25837..26604)
                     /gene="livG"
                     /locus_tag="K0W59_001018"
     CDS             complement(25837..26604)
                     /gene="livG"
                     /locus_tag="K0W59_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HBI3022154.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26601..27878)
                     /gene="livM"
                     /locus_tag="K0W59_001019"
     CDS             complement(26601..27878)
                     /gene="livM"
                     /locus_tag="K0W59_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HBI3022155.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27875..28801)
                     /gene="livH"
                     /locus_tag="K0W59_001020"
     CDS             complement(27875..28801)
                     /gene="livH"
                     /locus_tag="K0W59_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HBI3022156.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28849..29958)
                     /gene="livK"
                     /locus_tag="K0W59_001021"
     CDS             complement(28849..29958)
                     /gene="livK"
                     /locus_tag="K0W59_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HBI3022157.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLACSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30382..30765
                     /gene="panM"
                     /locus_tag="K0W59_001022"
     CDS             30382..30765
                     /gene="panM"
                     /locus_tag="K0W59_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HBI3022158.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30964..32067)
                     /gene="livJ"
                     /locus_tag="K0W59_001023"
     CDS             complement(30964..32067)
                     /gene="livJ"
                     /locus_tag="K0W59_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HBI3022159.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32338..33192)
                     /gene="rpoH"
                     /locus_tag="K0W59_001024"
     CDS             complement(32338..33192)
                     /gene="rpoH"
                     /locus_tag="K0W59_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HBI3022160.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33437..34495)
                     /gene="ftsX"
                     /locus_tag="K0W59_001025"
     CDS             complement(33437..34495)
                     /gene="ftsX"
                     /locus_tag="K0W59_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HBI3022161.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34488..35156)
                     /gene="ftsE"
                     /locus_tag="K0W59_001026"
     CDS             complement(34488..35156)
                     /gene="ftsE"
                     /locus_tag="K0W59_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBI3022162.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35159..36655)
                     /gene="ftsY"
                     /locus_tag="K0W59_001027"
     CDS             complement(35159..36655)
                     /gene="ftsY"
                     /locus_tag="K0W59_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBI3022163.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36805..37401
                     /gene="rsmD"
                     /locus_tag="K0W59_001028"
     CDS             36805..37401
                     /gene="rsmD"
                     /locus_tag="K0W59_001028"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HBI3022164.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37391..37660
                     /locus_tag="K0W59_001029"
     CDS             37391..37660
                     /locus_tag="K0W59_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HBI3022165.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37663..38022)
                     /locus_tag="K0W59_001030"
     CDS             complement(37663..38022)
                     /locus_tag="K0W59_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBI3022166.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38163..38789
                     /locus_tag="K0W59_001031"
     CDS             38163..38789
                     /locus_tag="K0W59_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HBI3022167.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38863..41061
                     /gene="zntA"
                     /locus_tag="K0W59_001032"
     CDS             38863..41061
                     /gene="zntA"
                     /locus_tag="K0W59_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HBI3022168.1"
                     /translation="MSTPDNHGKKAPQFAAFKPITTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41163..41408)
                     /gene="tusA"
                     /locus_tag="K0W59_001033"
     CDS             complement(41163..41408)
                     /gene="tusA"
                     /locus_tag="K0W59_001033"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HBI3022169.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41629..42294
                     /locus_tag="K0W59_001034"
     CDS             41629..42294
                     /locus_tag="K0W59_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HBI3022170.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42367..42924
                     /gene="dcrB"
                     /locus_tag="K0W59_001035"
     CDS             42367..42924
                     /gene="dcrB"
                     /locus_tag="K0W59_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HBI3022171.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42928..44145)
                     /locus_tag="K0W59_001036"
                     /pseudo
     CDS             complement(42928..44145)
                     /locus_tag="K0W59_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            44277..45326
                     /locus_tag="K0W59_001037"
     CDS             44277..45326
                     /locus_tag="K0W59_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBI3022172.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLYLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTRVCKIWMETTKGGSKL
                     AILLGPDRPKSRLPG"
     gene            45381..45968
                     /gene="acpT"
                     /locus_tag="K0W59_001038"
     CDS             45381..45968
                     /gene="acpT"
                     /locus_tag="K0W59_001038"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HBI3022173.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46079..47653
                     /gene="nikA"
                     /locus_tag="K0W59_001039"
     CDS             46079..47653
                     /gene="nikA"
                     /locus_tag="K0W59_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HBI3022174.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPLETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47653..48597
                     /gene="nikB"
                     /locus_tag="K0W59_001040"
     CDS             47653..48597
                     /gene="nikB"
                     /locus_tag="K0W59_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HBI3022175.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPTTL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48594..49427
                     /gene="nikC"
                     /locus_tag="K0W59_001041"
     CDS             48594..49427
                     /gene="nikC"
                     /locus_tag="K0W59_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HBI3022176.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49427..50191
                     /gene="nikD"
                     /locus_tag="K0W59_001042"
     CDS             49427..50191
                     /gene="nikD"
                     /locus_tag="K0W59_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HBI3022177.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50188..50994
                     /gene="nikE"
                     /locus_tag="K0W59_001043"
     CDS             50188..50994
                     /gene="nikE"
                     /locus_tag="K0W59_001043"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HBI3022178.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKNLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51000..51401
                     /gene="nikR"
                     /locus_tag="K0W59_001044"
     CDS             51000..51401
                     /gene="nikR"
                     /locus_tag="K0W59_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HBI3022179.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51521..51880)
                     /locus_tag="K0W59_001045"
     CDS             complement(51521..51880)
                     /locus_tag="K0W59_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBI3022180.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51877..52152)
                     /locus_tag="K0W59_001046"
     CDS             complement(51877..52152)
                     /locus_tag="K0W59_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBI3022181.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            complement(52211..53335)
                     /locus_tag="K0W59_001047"
     CDS             complement(52211..53335)
                     /locus_tag="K0W59_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3022182.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53335..56070)
                     /gene="rbbA"
                     /locus_tag="K0W59_001048"
     CDS             complement(53335..56070)
                     /gene="rbbA"
                     /locus_tag="K0W59_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HBI3022183.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQSFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQLTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56067..57134)
                     /locus_tag="K0W59_001049"
     CDS             complement(56067..57134)
                     /locus_tag="K0W59_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBI3022184.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKTAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57500..59122)
                     /locus_tag="K0W59_001050"
     CDS             complement(57500..59122)
                     /locus_tag="K0W59_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBI3022185.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNHGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKSQFADRTYFSNCSPVDPTSTRPTYFGDTDSSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGVQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINLGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
BASE COUNT        14066 a        16425 c        15074 g        13958 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa accaggaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggaatgg tttttatctt ccactgcagc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cgaatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccacacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgtttgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tgcctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggtgcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcaga cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 tttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tattacgggt aactgttatc ggtaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctggat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tagcaaaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgaa cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaagcca gcacccggct ggctaagtaa tactggaagc
    16861 aatgtgagca atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc gcgagacaat
    16921 aataatcatt ctcattcgca cttgtccaac actttttgca aaaaaatgca tttgactcgc
    16981 atttgaaagt caatgatgtt gaaagggaca tttaccccaa agaggacaaa ggaatgagtg
    17041 agatagtaat acgccacgca gaaacacggg attacgagac catcaggcag attcacgccc
    17101 agccggaggt gtattgcaac acactacagg tgcctcatcc ttccgatcat atgtggcagg
    17161 agcgactcgc cgatcgtccc ggcatcaagc aactcgtcgc ctgtattgat ggagacgtcg
    17221 tgggccatct caccattgac gtgcaacagc gcccacgccg cagtcatgtt gccgattttg
    17281 gtatctgtgt cgactctcgt tggaagaacc gcggcgtcgc cagcgccctg atgcgagaga
    17341 tgattgaaat gtgcgacaac tggttacggg tagatcgcat tgaactaacc gtgtttgtcg
    17401 ataacgcgcc agcaattaag gtctataaaa aattcggctt tgagattgaa gggaccggta
    17461 agaagtacgc attgcgtaat ggtgaatatg tcgatgcgta ttatatggcg cgggtgaaat
    17521 agagaatttg caatctaagt ttgtcattta agccctcacc taccgggagg gcttaagata
    17581 ttaatacccc gccgttaaat catccaccga gcgcgggtcg gatacgccgt acaactcacc
    17641 gtccggccca accataatgc tttgtgtgct gcccatcgcc tctttcagcg ccactttctg
    17701 accttttgct tccagcagct tgagcgtatc cgggctaaac cctttttcga cacgcagctc
    17761 gtccggcaac cactgatggt ggaaacgcgg cgcattggtc gcttcggcga cgttcatgcc
    17821 ataatcgatg ctattcacca ccatttgcag caccgtggtg ataatccggc taccgcctgg
    17881 gctaccggta accagccagg ttttaccgtc tttcaccaca atggtcggcg acatcgacga
    17941 cagcgggcgt ttgttcggcc cgacggcgtt ggcatcaccg cccaccagcc cgtaaacgtt
    18001 cggtacgccc ggtttggcgg agaaatcatc catctggtta ttaagcagaa taccgctctc
    18061 gcccgcgaca atacccgtac cgaaggtggt gttcagcgta taggtcaccg ccaccgcgtt
    18121 accgtcttta tccactacag agtaatgggt agtttgatta ctctcataag gcgcaagctt
    18181 gccagggcga atctcactgg acggcttcgc ttttttgata tcaatttgat cggcaatgga
    18241 tttggcatag gctttattgg tcagtgcctg ccacgggact ttgacaaaat ccgggtcgcc
    18301 aagatattcc gagcggtcgg cgtaggcgta tttctccgct tctgccatga tttgcatcgc
    18361 atcggcgctg ccaaagccgt atttcttcat atcgaagttt tccagaatat tgaggatttg
    18421 tacgatatgg atcccgccgg aggatggcgg tggcatggag taaacctgat acccgcgata
    18481 atcgccgctt atcggagtgc gttcgaccgc tttataggcc gctaaatctt ctttagtgat
    18541 caagccaccg tttttctgca tctcctgggc gatctgttcc gcaatcgtgc ctttatagaa
    18601 ttcgtccggg ccgttttcag caatcatctc caggctcttt gccaggttcg cctgcaccag
    18661 cgtgccgctc tttttcagcg gctcgccctc tttccagaag atagctttac tgttttcgtg
    18721 attcggcaac acttcgctac cgtaggtttt gagatcgtca gccagcgcgt cgttaacgat
    18781 aaaaccatcg cgtgccagtt taaacgcggg ctgcacgact ttgttcagcg gcatggtgcc
    18841 gtatttatcc agcgccagcg agaaacctgc taccgtaccc ggtgtgccgg aagccagatg
    18901 cgaagtgagt gattttttgc tgtccgggtt gccctgatca tcgaggaaca tatcgcgggt
    18961 cgctttggcg ggtgccattt cgcggaaatc gatagccgtg gtattgccat ttttcgagcg
    19021 gattaacata aaaccaccac cgcccagatt ccctgcctgc ggatgcgtta ccgccagcgc
    19081 gtagcccacc gccacggcgg catcaacggc attcccgccc tccttgagga tatccacccc
    19141 cacctgagtg gcagtggcgt ccacagacgc taccattccc tgtttcgcgc gtaccggatg
    19201 gaagacatct tcctccacac catacgagac gggcggcgct aaaacaactt cctgagagca
    19261 gagcagcaat ggccacccgg cgtaaaaacg tcggttttat catcgttatt ctccagagat
    19321 taaggggcaa ccccagctaa gcctggtata taactctgaa ttaatcatcg ttttgtcggg
    19381 aagcgagtaa acttaaagga tatcttcaga ggaggatacg cgatgaaacg acttctgatt
    19441 cttacggcac tcctgccgtt tgtcggcttt gcacagccca ttaatactct gaacaaccct
    19501 aaccagccgg ggtatcagat acccagccag cagcggatgc aaacccagat gcagactcag
    19561 caaatccagc aaaaagggat gctgaatcag caactgaaaa cgcaaactca gttgcaacag
    19621 cagcatttag aaaaccagat aaacaataat tctcagcggg tgttgcagtc acagccgggg
    19681 gagcgaaatc ccgcccggca gcaaatgctg cccaacacca acggcgggat gttaaacagc
    19741 aaccgtaatc cggatagttc gttgaatcag cagcatatgc tgccggagag gagaaacggc
    19801 gacatgctga atcagcccag cacgccgcag cctgacattc cgttgaaaac tattgggccg
    19861 taaagttcgg accaatcacg tcaatcgcat cggtacagat gcaatccacg ccccagcgca
    19921 gcaactctgc cgcgcgctgg ggtttattga cggtataaac cagaatccgt aatccggcgt
    19981 ctttcaactg catcactcgc gctttatcga gtaacttatg attgagatga atagagacgc
    20041 agcccagccg cgcggtcagt tcgcgccagt cgtcgcgcca ctcatcaagc aataaaccac
    20101 gcggcagttc cggtgccgcc tgttgtgcag cttctaaagc atcaatctca aacgatgaca
    20161 gcagcggcgg cgtcataccg gcccacagtt ggcgtgccgc cagcgccacc attttgcccg
    20221 ttaatggtcc agtgccagtg gtgggtttga tttcgatatt cgccatcatc ccgtgttcgc
    20281 gacagcgttc cgccacctgc gaaagcaacg gcagcggctc acctttaaac gctttgctgt
    20341 accaactgcc cgcatccacg cgcagtaaat cctgccagtt cagttcaccc gcgacgcccc
    20401 agccgttgct ggtgcgttcg agattgtcgt catggagcag gaagatctcg ccatctttcg
    20461 ataacttcgc gtcaaattcg atcatcttat gaccgtattt tgccccgacg tcgattgccg
    20521 ccagggtgtt ttccggggcc agcttaccgc cgccacgatg agcgacgatg cggggataag
    20581 gccagttact catactcgtt gtcctgtttc accatcaaaa agatgcagct gattttccgc
    20641 cagatgcagc cacagcgtgc tgcctgccgt cgggcgctcc tgatgcgcca gtcgcaccac
    20701 cagcttctgc ttgcgagctt agcgcaatat gttccgggcg aatgccgagg gtcattttac
    20761 gcccggcata ctgacggtag ccaccgttta gcggtagcac gataccgccg tccagttcga
    20821 aatgcgtgcc ttcattattc atgcggcctg tcagcaggtt catcgccggg ctgccgataa
    20881 aactcgccac aaacaggctg gcgggctttt cgtagacttc caccggcgtg ccaatctgtt
    20941 cggcaacgcc gccgttcatc accattactc gctgggcgag cgtcatcgcc tcaacctgat
    21001 cgtgagtaac gtagagtgaa gtcgttttca ggcgacggtg cagctgttgc agttcaagac
    21061 gcatctgtac gcgcagcttg gcatcgaggt tagagagtgg ctcatcaaac aagaacaccg
    21121 ccggatcacg cacaatcgcg cgtcccatcg ccacacgctg gcgctgaccg ccggaaagct
    21181 cgcgcgggcg acgtttgagc agaccgtcca gctccagaat gcgcgccgct tctttaacgc
    21241 gctcggctat ttgctgcttg cccatgccgc gaattttcag cccccacgcc atgttttctt
    21301 cgacgctcat atgcggataa agcgcgtaat tctggaacac catcgcaatc ccgcgatctt
    21361 tcggctccat ttcggtcacg cgctggtcgt tgatccagat atcgccttcg gtcaccctct
    21421 ccagcccggc aaccatacgc agcagcgtcg atttcccgca gccagacggc ccgaccatca
    21481 cgataaattc gccatccgcc acatcaaggg tcagcggttt aatgacctag gttttgccat
    21541 cccagctttt ggttactgcc tgtaatttca gtcctgccat cttatttctc actatcgacc
    21601 aggccgcgca cgaaggcacg ctgcatcact aaaacaatca ctactggtgg gatcagggtt
    21661 aacaacatcg ccgccatcac tgagttccat tccgtggtgc cttcgccagt ggcgatcatc
    21721 cctttgatcc ctgccacggt ggtgccgaga tccacgtcgg taataatcaa caacggccac
    21781 aaatactggt tccagccgta aataaaggtg atcacaaaca gcgccgccag attagttttg
    21841 gagagcggaa aaacgatgtc gcaaaagaag cgcattggtg acgcaccgtc gatccgcgcc
    21901 gcttccacca gttcatccgg cagcgtcata aagaactggc gaaacagaaa cgtggcggtc
    21961 gccgaggcca tcagcggcag cgttaaaccg gcgtagctgt cgagcatctt caggttggcg
    22021 atgacttcca ccgtcgggaa gatacgtact tcaaccggca gcatcagggt gataaaaatc
    22081 atccagaaga agaggttacg tagcggaaaa cgaaaccaga caatggcaaa tgccgagagc
    22141 atcgagacag taattttgcc gagcgtaatg ctgaacgcca tcacaaagct gttaagcaac
    22201 atccgccaga acggcgcgct atttgtgcct accccgttca cccagatgtt gtggatgttt
    22261 tccagcagat gcgtgccggg gatgagcgtc atcggcgcgg cgtagacggc ctgtttatcc
    22321 agcgtcgccg cgacaaacgc cacgtacagc gggaagagga tcaccgcgat cccgaggatc
    22381 agcatggtat ggctgaatat cgtcagccac ggacggttct caatcattgg taacgcacct
    22441 tgctttcaac atagcggaac tgcaccaccg tcagcacgat gacgaggaac atcaacacca
    22501 ccgactgtgc ggcagacgaa gccagatcca gtccggtaaa accttcgcgg tagatcttat
    22561 aaatcagcgt cgtggtggcc tgaaccggcc cgccggacgt ggcggcgtcg atcaccggga
    22621 aggtgtcgaa gaaggcatac accaggttca ctaccagcag gaagaaactc accggggcga
    22681 taagcggcag cgcaatctta aagaagcggc gaatcggccc tgcaccgtcg atggctgcgg
    22741 cttcgatcaa cgaacgggga atggattgca gcgcggcata gaagaacaga aagttgtagc
    22801 tgatttgctt ccacaccgag gcaaacacca ccagaaacat cgcctgaccg ctattttgcg
    22861 cgtggttcca gtcgtagccg aactcagcga gaaaatgggt gatcagcccg cgaccggggt
    22921 taaacaggaa gatccacaat acggcggcaa cggcgggagc cacggcatac ggcagcaaca
    22981 ttaaggtttg gtagaaacga ctgccgcgca cgatgtactc caccagcgcc gcaaagaaca
    23041 gcgataccag caaaccgctg acggtgacaa aggtgctgaa ttttatcgtc gtccagaagg
    23101 agtcgagata atagctgtca tgaaacagcg tgacgaagtt atccagaccg acaaactggc
    23161 tggagaaacc aaacggatcg acgctttgta gcgagtacca caacgcttcg cccgcaggcc
    23221 agataaaaaa gataacggtg atgatgagct gcggcgcgac cagcagataa ggcagccagc
    23281 gcgagcggaa caccggacgg gatgatgaca ttacggttaa ttcctgaact gtgccggatg
    23341 cgcttcgctt atccggccta cacgcgatgc caggtcagat aagcgaaacg catccggcat
    23401 tacactgatt aagacttcgt cgatttctca aagcggcgca gtaactggtt cccacgctca
    23461 actgcggtat ccagcgcctg ctgtggcgtc ttcttaccgg tccacacgct ctcaagctct
    23521 tcatccacaa tcacgcggat ctgcggcatg ttgcccagac gaagcccttt ggtgaacggc
    23581 aacggcggct tgttcagcat ctgacgcgtc gcggtatccg cacctgggtt tttctcgtaa
    23641 aagccctgct cacgggtcag gtcatacgcc gctttggtaa ttggcagata accggttttc
    23701 tgatgccact cagcagcgtt ttctggcttc gcgaggaagt cgagaaactt cgccacgccg
    23761 gtatacgttt ctttatcttt gccctgcatc acccacaggc tggctccgcc gataatggcg
    23821 ttttgtggcg catctttcgc atcggcgtcg taaggcatca tacctacgcc gtagttaaat
    23881 ttggcgtact cgcgaatgtt ggcaagagaa ccggaagagg cggtggtcat cgcgcaatca
    23941 ccgttataga acttctcggt ggattcatcc ttacgaccga cgtagctgaa gtcgcccttc
    24001 ttgttcatct cctcgagcat ggcgatgtgt ttcacctgct ccggcttgtt gaactccagc
    24061 accgcgtccg tgccgtcaaa gccgttgttt ttgctggcaa acggcagacc gttccaggcg
    24121 ctaaagtttt ccagttggat ccagccctgc cagccgctgg cgtagccgca cttaataccg
    24181 gaggctttca gttttgcgga atagtccgcc agatcctgcc aggttttcgg cggctgttcc
    24241 gggtctaacc ccgctttctt gaaggcgtct ttgttgtaat agagaacggg agtcgagctg
    24301 ttgaacggct gggagagtaa gtggccggtt ttgctgtcgg agtagtaacc ggaaaccgtc
    24361 ggcacaaact gcgactcatc gaactgaatc cctgcctctt taaacacgtc atacaccggt
    24421 ttaatggctt tcgacgccat catggtggcg gtgccaactt cataaacctg caaaatagcc
    24481 ggagcgttgc cggtacgaaa tgcggcaatc cccgcgctta aattctgttc gtagttgcct
    24541 ttataggtcg gtacaatttt gtaatccgga ttttcggcgt taaaacgttg ggccagagaa
    24601 tccacctctt tacccagttc cccttccata gaatgccaga acggaatggt cgtcactgcc
    24661 tgtgcattcc ccattaacgc cagtccgagc gccagtgctg aagctgtata atgtaacggt
    24721 ttcatcgttt atctctcttg ttgtaccgaa tgcgcgaatt cacgcgtttt atgctcgcgg
    24781 ggtaacatga catgctcgaa ttacagaaaa ataacttttt tgttacattt gtaagatagt
    24841 aaggtgtcag aaagatgaca aggcggtgac ggcgtgggtg agggaaaatg ggagatgggg
    24901 cacggataag cggaaaaata gaaggtctga atcaaactct acagattact catcgtttca
    24961 tgccggatgc ggcgtaaacg ccttatccgg cctacaagat cgtgcaaatt caatatattg
    25021 caactcaccc agtaggcctg ataagcgcag cgcatcaggc aattttgcat ttgtcacctg
    25081 tctcaaagga gtcttttgac tccttatcaa tcaacgtgtt attacccgcc taaatacgca
    25141 cttctcactg cttcgttcgc cagcagcgcg tcgccggtat cggagagcac cacatggccg
    25201 ttttccagca cgtagccgcg atccgccagc tttagcgcct ggttggcgtt ctgctcgacg
    25261 aggaagatag tcatcccctg ctcgcgcagc tgctcgatgg tgtcgaaaat ttgctggatg
    25321 ataatcggcg caagaccgag cgatggctca tcaagtaaca acaaacgcgg attgctcatc
    25381 agcgcgcgac caatcgccag catctgctgt tcaccgccgg acatggtgcc cgcccgctga
    25441 acacggcgct catgcagacg tggaaacagt tcatacaccc actttatgcg ctcctgaaac
    25501 tggtcacgtt cggcaaagaa gccgcccatc gccaggttct cttccaccgt catccgcgag
    25561 aagacgcgac gcccttccgg gacaatcgct accgcttcgc gcatgatttt cgccgtctgc
    25621 cagtcggtaa tgtctttatc atcaaacaca attcgcccgc tggtggcgcg cggatcgccg
    25681 cataacgtgc cgagcaaggt ggttttcccc gccccgttcg cgccaatgag agtgacaatc
    25741 tcgccctgat tgatatgcag gctcacctca tgcagcgcct ggattttgcc gtagtgggcg
    25801 ctgactttgt caaaggacaa catgactttt tccatcttat gcctcaccta aataggcacg
    25861 gatcacgtcc gggttattac ggatttgctc cggcgtaccg tttgccagtg gcgtcccctg
    25921 attgaccaca taaattcggt ctgaaatccc catcaccagc ttcatatcgt gttcaatcaa
    25981 caagatagtg gtgttgtgat gattgcgcag ttcggcaatc agctcatcca gctctttggt
    26041 ttcttttggg ttaagacctg ccgccggttc gtcgagcatt aaaatctccg gctgcgtcac
    26101 catgcagcgg gcaatctcca gacggcgttg gtcaccatag gccaggttac tcgcctgacg
    26161 gttggcgtgt tccagcaaac caatgcgctc aagccaggtg gcggcgcggt cgagcgcttc
    26221 gctctgggcg cgacggaaag aaggcgtttt caacaggcca gagaacagcc cggttttcag
    26281 ttgctgatgc tgcgccacca gcaggttttc aattaccgtc atttcacgga acagacgcac
    26341 atgctggaag gtgcgcacca cgcccatgcg agcaatttgc tgccccggta aaccttcaag
    26401 atgctgatcg cgcagtaaaa tggtgccgcc ggtgggtttg tagaaaccgg tcaggcagtt
    26461 aaaaaccgtg gtttttccgg caccgttagg gccgattaac gagacgattt cctgcgggta
    26521 cagttcaaga ttgacgttgt tcaccgccag caggccgccg aagcgcatca tcagaccgtt
    26581 aacagataat aatggctgac tcatgcctgc tctcctttcg ctgcgccgtt tttcagcttc
    26641 agttgcgggc gcgtcatggg cagcaaaccc tgcggacgcc agatcatcat cagcaccatc
    26701 aaaccaccga gcattaacat gctgtattcg ttgaaatcac gcatcaattc gcgcgacacc
    26761 accagcagaa cagccgccag aatcactgca aattgcgagc ccataccgcc gagcaccact
    26821 atcgccagca caaaggccga ttcagcaaag gtgaaggatt ccgggctgac aaagccctga
    26881 cgcgccgcaa acagcgttcc ggcaaaaccg gcaaatgcgg cgctgatggt aaaagcggtc
    26941 agcttgatac ggcgcgggct taagcccagc gaacgacagg cgatctcgtc ttcacgcaac
    27001 gcttcccacg cgcggcccag cggcatccgc agcaggcggt taatgacaaa caggcttaac
    27061 accaccagca gcaacgccac caggtagagg aagataacgc gatcggacgg atcgtatttc
    27121 aggccaaaga aattactgaa cgtgtcccag ccgccttcac gagcggtacg gctgaactcg
    27181 agtccgaaga gtgtcggttt cgggatctga ctgattccgt tcgggccgcc ggtgatttca
    27241 gtgttattga gcagcaatat gcgcacaatt tcgccgaaac caagggtaac gatcgccaga
    27301 tagtcaccgc gcagacgcag caccggaaaa ccgagcagga aacccgccgc cgctgccatc
    27361 agtccggcaa tcggcaaaca ggtccagaac cccagaccgt aatagtgatt gagcagcgca
    27421 aaagtgtaag cgccgatggc gtaaaaaccg ccgtacccca gcaccagcag accagaaaga
    27481 ccaacaacca cattcagccc gagaccgagg ataatgtaga tcatggtcag agtggcgata
    27541 tccaccgtcc cacgtgacac cataaacggc cacgccacca caagcaccag cagcgccacg
    27601 aggaacagct tttgcttcac cgtggaccca tcaatcgcgg gcagaataaa cttcggcccg
    27661 gaaacgcttt tcaacccttt ctggaaagcc ggtcgcaaaa gctggaagaa aaagaccacc
    27721 gccgtgccga taaacatcca ctgccaacgg atgtctgaag ccgtgtcgac caccagtttg
    27781 gtgccatcca gctccagttg tacgcccata aagacgcccg ccaggacaaa gaacatcgcg
    27841 gcagagagca gtgccattgc aatatgcatc ggtttcatac tttctctacc tccgggcgac
    27901 ccagaatacc ggtcggcatc accagcagca ccagaatcag cagggcgaat gagaccacat
    27961 ctttatattc cgtactcaga taggcagaag agagcgcctc cgcaatcccc aggatcagcc
    28021 cgccaatcat cgcccctgga atgctgccaa tcccaccgag caccgcagcg gtaaaggctt
    28081 tcatcccggc cataaagccg atataggggt taatgacgca gtagaactga ccgagcagta
    28141 cgccagccac tgccgccatc gccgcgccaa taacaaaggt caaagcaatc acccggtcgg
    28201 tgttgatgcc aagcaggctc gccattttca gatcttccgc gcaggcacga caggcgcgcc
    28261 ccatacggga atagcgaatg aaaatcgtca gcgccagcat ggcgaggaag gtgacaatcc
    28321 agatcaccgc ctgcatggtg gtaatagagg cagagaagtt ttcgctatgg cccaccaccc
    28381 actgaccgtt aaacaggctc ggcagcgcca cgtcgcgcga accttcggtc aggctgacgt
    28441 agttttgcag gaagatggac ataccgattg cggaaataag cgcaatcagg cgcttagagt
    28501 tacgcaccgg gcggtaagct acccgttcga tactccagcc gtaggcgctg gcgatgacga
    28561 ttgcgccgac gaatcccgcc gccaccagca gccagccggt atcaatgccc atcatcatca
    28621 gcgcggcgat gatcataaat gagacgtagc tgccaatcat ataaacctcg ccgtgggcga
    28681 agttgatcat gccgataatg ccgtaaacca tggtgtagcc gatggctatc agcgcgtagg
    28741 tactgcccag cgtgacgccg ttaaacatct gctgcaagaa atacaaaaac tgctcagaca
    28801 taaggtaacc tttctaaacc cgcccgcatt ttacgggcgg tgggatgatc acttggctgc
    28861 cgtggatgaa ccgtcggcgt gccactggaa gacaccaaaa tcaaatccct taagatcgcc
    28921 tttttcatcc cagttcagcg gcccaatcac ggtgtttgca ccgttagctt ttaaatcttt
    28981 caccagcgcc agcggctcat cgctgccggt acgctcaagg gcagttgcca gagattgcac
    29041 tgccgcgtaa gtgatccaga cataaggccc ggacggatct ttcttgtctg ctttcagcgc
    29101 atcaacgatg ccctggtttg ccggatcctg gtcatagcgt tttggcatag tgaccaacat
    29161 gccttcggcg gcatcaccgg caatgttcga cagcgatgcg ttaccgacac cttccggccc
    29221 cataaactgg gttttcaggc caacggaacg ggcttggcgc agcatctgcc ccatttccgg
    29281 gtagtaaccg ccgtagtaaa cgaagtcgat attttctttt ttcaggcggg cgatcagcgc
    29341 ggagaaatct ttctccccgg cggtaatacc gtcgaagaag acgacgttgg cgttagccgc
    29401 tttcagcccg tcctgcaccg aacacgccag cccttcgcca tactgttgtt tgtcgtgaat
    29461 gatggcgatg cgctggggct tcaccgtctc aagaatgtat tttgccgccg taggcccctg
    29521 ggaagagtcc agcccggcag tacgcataat gtgttgataa ccgcgttggg tcagctccgg
    29581 gttggtcgct cccggcgaga tcatcagaat accttcgtct tcgtagatat ccgacgctgg
    29641 ctgggtagag gaagaacaca gatgaccaat aacgtattta atgccgtcgt taacgatttt
    29701 gttggcgacc gcaacggctt gtttcgggtc gcaggcgtcg tcatattcca cgccaaccag
    29761 cttgtcgccc ttaattcccc ctttgacatt aatgtcttta atcgcctgac gcgcgccgtt
    29821 aaattccata tcgccccact gggcaatcgg gccggacatc gcgccgacaa cggcgacttt
    29881 aatatcgtca gccatagcgg tgtgtgaaat tgccagtgca atcatccctg cgatgatagt
    29941 tttcgcattc cgtttcatag tcaaaaatcc ccattcgtga tgttgtgttg ctttgttttt
    30001 atgtgttaac aaatcagact gttctttttt tatactgcac tgtttttgcc tgtctgatta
    30061 aggggttagc gcagtatttt gtgatgatag cgattaaaat ccctattttt cagtcgatta
    30121 agaacagata atattctgaa tttattgata gataaacaga aaaaagtgcc tttgtcagca
    30181 taaaataacg gcacaaaggg cggaataatt cactatcatt caggggatta tgctggacat
    30241 ttttcattct ctaatgtttt aactttgtaa ttattgctgt taaaaaatta atcacctgcc
    30301 aaaagaaata aaaaagagaa agcctccgat taaattattt cgctacactg gttccacttt
    30361 tgtgatttac acgggttacc catgaagctg accatcattc gattagaaaa ctttagcgac
    30421 caggaccgga ttgacctgca aaagatctgg ccggagtatt ccccttcctc tttacaggtt
    30481 gacgataacc accgtatcta cgccgcgcgt tttaacgagc gtctgcttgc ggccgtgcgg
    30541 gtaaccttaa gcggcaccga gggagcactg gattctctac gcgtgcggga agtcacccgc
    30601 cgtcgcggtg tggggcaata tctgctggaa gaggttttgc gtaacaatcc tggcgtttca
    30661 tgctggtgga tggcggatgc aggcgtggaa gatcgcggtg tgatgacggc gtttatgcag
    30721 gcactgggat ttacggcaca gcagggcggc tgggagaagc gttaatcgtc aagttgattt
    30781 caaaagtgat gaccaacgag aaattgcctg atgcgctacg cttatcaggc ctataatgtg
    30841 tgctgcaatt tactgatttc tttggttctt gtaggccgga taaggcgttt acgccgcatc
    30901 cggcatgaag caacgtattc gatattagca atttggcggc aacccaaagt tgccgattaa
    30961 caattacttc gcatcggtcg ccgtgccgtt ggcgtgccag tcaaatacgc cgaactcaaa
    31021 gccctttaga tcgcctttct catcccaggt cagtggcccc atcacggtat ccacggagtt
    31081 cgcttttagg tatttggcga tttcagccgg atcgtcagac tgattcaggc ccgcctgcaa
    31141 agattgcagc gcggcgtagg tggtccagac gaatgcgccg cttgggtcct gttttttcgc
    31201 tttgatcgca tcaacaatgg gtttgttcgc cggaacctga tcgtagttct tcggcttggt
    31261 caccagcagc ccttccgctg attcgcccgc aatgttagac agcgaaacgt tagccacacc
    31321 ttccggcccc ataaactgag ttttcagccc tgccgcgcgt gcctgacgca ggatttgccc
    31381 catttccggg tgataaccgc cgtagtaaac gaagtcgata ttctcttttt tcagacgcgc
    31441 caccagcgtt gagaaatctt tttccccggc ggtgatacca tcaaagaaca ccacgttagc
    31501 attgcctttc ttcaggccgt cctgcaccgc tcgtgccaga ccttcgccgt attgctgttt
    31561 gtcgtgaacg atagcaatac gctgcggttt cactttctca agaatatatt tcgccgccgt
    31621 cggcccctgg tcggagtcca ggccggtggt gcgcaggatc agctgatagc cacgggcggt
    31681 cagctccggc gcggttgccg ctggggtgat cattaaaatg ccttcgtctt cgtagatgtc
    31741 agacgcaggc tgcgttgatg aagaacagag gtgaccaatc acatatttaa tgccgtcgtt
    31801 aacgactttg ttcgccaccg caaccgcctg tttcgggtca caggcatcgt catattttac
    31861 gatttgcagt ttgttgcctt taatgccgcc tttagcgttg atatccgcaa ccgcctgctc
    31921 tgcgccggta aactcctggt caccgtactg cgcaaccgga ccggacattg cacccacgac
    31981 cgcgacttta atatcttctg ccagagccat attgctgaat gccagcgcga tacatcctgc
    32041 cagtaacgct ttacccttta tgttcatcct gagaatcccc attcttctgg ttattacgtg
    32101 tgttgtgatg ttgttgttca gcactttatt tcgttttatg catgactacc cgtgctttag
    32161 cagcatactc tgctaaaaca tacccgattt ttatgatatt ggaatagcta ttttgacagt
    32221 ttattaacaa tctgcgtggg gattggcgtt ttgccggagg gggaattgat tattgtagag
    32281 gcccaaaaaa caaaaacccc ggactctcat ccagggttct ctgcttaata gcggaaatta
    32341 cgcttcaata gcggcgcgaa gttttttcat cgcgttcttt tccagttggc gcacacgttc
    32401 agcggaaaca ccgtaacggt cagccagttc ctgcaacgtg gacttgttgt cttcgtccag
    32461 ccagcgcgca cggatgatgt cctggctgcg ttcgtccaga ccatgcatcg cgtcggtcag
    32521 acggtttgcc gcctgctctt cccagttatc atcttcgatg ccgtcggcaa agttagatga
    32581 tttatcctgc agatagagca ccggtgccat cggctggctg tcggaatcgt cgtcggaaga
    32641 caggtcaaag gtcatgtcct gtgccgccat acgtgattcc atctcacgta cgtctttgct
    32701 ggttacgccc agttcacggg ccaccatttc gacttcatcc tggttaaacc agcccagacg
    32761 ctgcttggtt ttacgcaggt tgaagaacag tttgcgctgc gctttggtgg tcgcaacttt
    32821 gacgatacgc cagttacgca gaacgtattc gtggatctct gctttgatcc agtggacggc
    32881 gaaggagacc aggcgcacac ccacttccgg gttaaaacgg cgcactgctt tcatcaggcc
    32941 gatgttacct tcctgaatca aatccgcctg tggcaggcca tagcccgcat aattacgagc
    33001 aatatgaaca acaaaccgca ggtgagacag gatcagcgtt ttagctgctt ccagatcgcc
    33061 atggtaatgc agcttttcag ccagcgcccg ctcctcgtca gccgacaaca tcggccacgc
    33121 gttagctgcc cggatgtagg aatccaggtt gccaactggg gctaaagcta aactttgcat
    33181 tttgtcagtc attcaaatcc tctcaatcga tatcttctgg cgcttcagtg gtagcaacaa
    33241 ctgtgccaaa gcttaagagc aacgaggtta tcattcactg ttttatcaga ccgtgatttt
    33301 atccacaagt tcaatgcaag cttgtgaata aattacgcac aaaatgtgac atagagatga
    33361 aataccggga agagacaacg gggtctcttt ccctgctacg gaacccattg cagggaaaga
    33421 gtataacacg cttttattat tcaggcgtaa agtggcgtaa atgttgtacc gtggcaagcc
    33481 acgctgccac ccagccaatc atcgagcata ccagcagcaa tagcaggcat tcatcgaacg
    33541 ataaaccatt gatatcaaac ttcgttccga aaacctgtgc cacttccgcc accgctgacg
    33601 acaaacgcag taccagaatt tctgacaaaa ttaatgacaa caatgcgcca gaaaatccca
    33661 gcagcgcacc accgtacagg aacgggcgaa ggatgaaacc atccgtcgca ccaatcagtt
    33721 tctgcacgtt gatggagtca cggcgagcaa agatgctcag acgtacgctg ttgccgatga
    33781 cgaggaacac tgccgccacc atcaacacgc cgatcatcgc cgaaacgcgc ccaaccagcc
    33841 cggtcaacgc cgccagacgg gcaaaccagc tgtcatccat ccgcacttcg tcaatgccgt
    33901 taatctgcgt gatacgatca cgcagcgtat tgagtgattc cgtcccctga aaatcgagtt
    33961 tcgggatcac caccgccact gccggaagcg ggttttcttc cagcatatcc agcgcaccac
    34021 caaaaccaga ccagttacgg aactcgccca gtgcgtcttc acgagaaaga tagttcactt
    34081 tctccacgcc ttgctcggcc tgcaactgtg ccaccacgcc cgcagcggcg tcatcgtcca
    34141 gcgttttttg caagtaaaca gtgatttgcg gtgacggata atactgcgtc gccgcctgat
    34201 taacgttttt gtacaccata taacagacgc tgggcagcgt cagggagatg gcgataacca
    34261 tcaccgttaa aaacgtggcg aacggtttgc ttttcagatc ctgcaatgcg ccgtggaagg
    34321 catagcgcac ctgttcgttg aaaacgttgg ttttgcgatt taccggtttt ggcgaggatt
    34381 tcgcgcgttt cggtgcgtta cgaccgccgt cgcctgagcc gccgaccgat ttacggaagc
    34441 gatccagacg cccaccaaac tgccgaatat gattgattgc atcgcgctta ttcatggccc
    34501 acgcctccat gcaagtgacc atcgctcagg gtgagcatgc gataggaacg ccgtgaaatc
    34561 aggttaatgt cgtgcgttgc catcaatacg gttaccccaa cgcggttaaa ctcttcaaac
    34621 agacgtagaa tcccttccga caacgcgtcg tccaggttac cggtcggttc gtccgccagc
    34681 agtaccgcgg gcttattcac caccgcgcgg gcaatgccaa cacgctgttg ttcaccgccg
    34741 gaaagctgaa tagggaagtt cttcgctttg tccagtagcc cgactttatc cagtgccgcc
    34801 gacacccggc gacgaatatc gtcaccgctg gcaccagcga taatcagcgg gatcgccacg
    34861 ttatcgtaga cagtacggtc catcagtaga tggtgatcct ggaaaatcat gccaatctgg
    34921 cggcgcagaa acggaacttc acggtttttc agacgcgtga tgtcatggcc gctaaaccag
    34981 attttcccgg cgctgggccg ctcaatccca cagatcagct tcaggagggt acttttccct
    35041 gcgccggaat gaccggtcag aaacgccatc tcacccggct gcatatggaa cgtaacgccc
    35101 tgcagcgcct gtctcccacc gagataagcc ttgctgacat gttcaaagcg aatcattgtt
    35161 aatcctctcg ggcaaaaagt gcctctataa agtcgtccgc cttaaacgga cgcaaatcct
    35221 caatacgttc gccgacacca atgtagcgga tagggatacc aaactggtca gccaccgaga
    35281 aaattacccc gcctttcgcc gtgccgtcca gtttcgttag cgtgatgccg gttaagccaa
    35341 cagcttcgtg gaacagtttg gcctggctta ccgcgttctg cccggtgctg gcatcaatag
    35401 tcagcataac ttcatgcggc gcttcaacgt cgagtttctt catcacgcgg acgattttct
    35461 tcaactcttc catcagatgc gatttgttct gcagacgtcc ggcggtatcg gcaatcagga
    35521 cgtcgatatt acgcgcttta gctgcctgaa tggcgtcgaa gataacagag gcggaatccg
    35581 caccggtatg ctgggcaatc accggaatat tgttgcgctg accccagacc tgaagctgtt
    35641 caaccgcagc tgcacggaaa gtatcgcccg ccgccagcat caccgattta ccctgctgct
    35701 caaactgacg cgccagctta ccaatcgtcg tggttttacc cacaccgttg acgcccacca
    35761 tcaggatcac gaacggcgtt ttatcttcaa cattcagcgg ctcatcgact ttcgccagaa
    35821 tctcacccat ctcttctttc agcaggccat agagcgcctc ggcatcacga agctgcttgc
    35881 gggatgcgcc ttccgtcaga ttggtgataa ttttacgtgt ggtttccacg cccacatcgg
    35941 cgatcaacag ctgttcttcc agctcttcaa acagatcatc gtcgattttt ttaccgcgga
    36001 acaggctgat aaatccggaa ccgagatttt ctttggtttt taacaggctg cgtttcaggc
    36061 gcgcgaagaa gccttctttg gtcggttttt cttgctcctg ggcaatttct tcaaccggct
    36121 gttcttcttc tgccggagga actaccatca ccgcctcttc tgccgcttcg gcagccagcg
    36181 cctgtgcttc cagctcttcg tcggtgattt cttctttagc cgcttcttct tctgccgctt
    36241 cgacaatctc tacggtttcc gcttcagcct gccactcttc tggcgaaacc tcttcggcgt
    36301 tgacgtcttc cggcaacggc agctcttcac gttcgatagc cactggctcc ggcgtttctt
    36361 ctacgaccgg ttccggctgt gcaacaactt ccgcttcagg ctgcgctttt tcactttcaa
    36421 caacctgttc agtgacttcc acaacgtcgg cagcaaaagt ttccgcctcg gcttcagtat
    36481 gcgcctgcgg ctgctcttca acggcttgtt cagaagcctt cgcaggctct tgcgcctgaa
    36541 cgatttcttc tacaaccggt tgttcattct gaacttctgt ctctttttcc ggggtctgct
    36601 ctttttgacc aaagcccagc caggaaaaaa agccacgtct tttttctttc gccatttgcg
    36661 actacactcc tcgctgttgc ttcatggcac agcgttaacg ctatgtacat agcagctaaa
    36721 aaaatgatga aatagtctat cacttaactt aattcacatc accgcctgca agtatgtgtt
    36781 atctggcgga ttgagcaatt tatcatgaaa aaaccgaatc attccggcag cggccaaatc
    36841 cgcattattg gcgggcagtg gcgaggccgt aaactcccgg ttcctgatag cccaggtctg
    36901 cgccccacca ccgaccgcgt acgcgaaacg ttgtttaact ggctggctcc ggtcattgtt
    36961 gacgcccaat gtctggattg cttcgccggg agcggcgcgc tggggctgga agcgttatcg
    37021 cgctacgctg cgggggcaac gttgattgag atggatcgcg tggtttctca gcagttaatt
    37081 aagaatctgg cgacactaaa agcaggcaat gcacgcgtgg tgaacagcaa cgcaatgtca
    37141 ttcctggcgc aaaaaggcac accgcataat atcgtgtttg tcgatccacc gttccgccgt
    37201 ggcttgttag aagagacgat aaatttactg gaagataacg gctggctggc tgacgaagcc
    37261 ctgatttatg tcgaaagcga agtcgaaaac ggtctgccca ctgttccggc aaactggtca
    37321 ttacatcggg aaaaagtggc gggtcaggtg gcttatcggc tgtatcaaca cgaagcacaa
    37381 ggagaaagtg atgctgatta atattggtcg tttgttaatg ctctgcgttt ggggattttt
    37441 aatcctcaat ctggtgcatc ccttcccgcg cccgctaaat attttcgtta acgtggcgct
    37501 gatttttacc gtgctgatgc atggtatgca gctggcgcta ttgaaatcca ctttaccgaa
    37561 agatggcccg cagatgacca ccgccgaaaa ggtgcggatt ttccttttcg gcgtgtttga
    37621 actgctggtc tggcagaaga aatttaaagt taaaaaataa ccttattgtt cgccaacaaa
    37681 gctaacaaag cgcgttcctt tatagctcag cgtaccttta tcgcccacgg tcagggcgtg
    37741 gtactgctgg gcgtcgagac gaaacgtctg ctccattcct ccgctttgcg gtttgaagct
    37801 tgcctcatag cgcatactgg tgcctgccgg agtcacttcc tgctggcgcg aacggcgatc
    37861 gttaatcggt ttttcccgct tgttgctcac caccaccagc ttttgctgga gcggagccat
    37921 atcattatca gctttttccc gtcgctgttg cataaaacga aacgatgcgg cgacgacaat
    37981 taagccaatg ataacaataa agaaaagagg tggtttactc atctttatcc ctcatcggaa
    38041 aatgcggaat taagcatacc ctgccagtta tggtgttgtc atccgtccac cctcgctact
    38101 aaactggaag caagaccgta ggcattccgc ttacgaaaaa ataacgaatt caaggaacta
    38161 agatgctttg gtcgtttatc gctgtctgtc tttccgcatg gctatctgtg gatgcatcgt
    38221 atcgtgggcc aacctggcaa cgctgggtgt ttaaaccgtt aacccttctt ctcctgctgt
    38281 tactggcctg gcaagcaccg atgttcgacg ccattagcta tctggtgctg gcgggactgt
    38341 gcgcctcact gctgggcgat gcgctaaccc tgttgccacg tcaacgtctg atgtacgcca
    38401 tcggcgcgtt tttcctctcg cacctgctgt acaccatcta tttcgccagt cagatgacgc
    38461 tctctttctt ctggcctctg ccactggtgt tgctggttct gggtgcgctg ctactggcga
    38521 ttatctggac gcggctggaa gagtaccgtt ggcctatctg cacgtttatc ggcatgacgc
    38581 tggtgatggt gtggctggca ggtgaactgt ggttcttccg tccgaccgct ccggcgctct
    38641 ctgcgtttgt tggcgcttcg ttgctgttta tcagtaactt tgtctggctg gggagccact
    38701 atcgccgacg cttccgtgcg gataacgcga ttgctgcggc ctgctacttt gccggtcact
    38761 tcctcatcgt ccgctcactg tatctctgat aaaacttgac tctggagtcg actccagagt
    38821 gtatccttcg gttaatgaga aaaaacttaa ccggaggatg ccatgtcgac acctgacaat
    38881 cacggcaaga aagcccctca atttgctgcg ttcaaaccga taaccacggt acagaacgcc
    38941 aacgactgtt gctgcgacgg cgcatgctcc agctcgccaa cgctctctga aaacgtctcc
    39001 ggcacccgct atagctggaa agtcagcggc atggactgcg ccgcctgtgc gcgcaaggta
    39061 gaaaatgccg tgcgccagct tgcaggcgtg aatcaggtgc aggtgttgtt cgccaccgaa
    39121 aaactggtgg ttgatgccga caatgacatc cgtgcccagg ttgaatctgc ggtgcaaaaa
    39181 gcgggctatt ccctgcgcga tgagcaggcc gccgacgagc cacaagcatc gcgcctgaaa
    39241 gagaatctgc cgctgattac gcttatcgtg atgatggcaa tcagctgggg tctggagcag
    39301 ttcaatcatc cgttcgggca actggcgttt atcgcgacca cgctagttgg gctgtacccg
    39361 attgctcatc aggcattacg gctgatcaaa tccggcagct acttcgccat tgaaacctta
    39421 atgagcgtag ccgctattgg tgcactgttt attggcgcaa cggctgaagc tgcgatggtg
    39481 ttgctgctgt ttttgattgg tgaacgactg gaaggctggg ccgccagccg cgcgcgtcag
    39541 ggcgttagcg cgttaatggc gctgaaacca gaaaccgcca cgcgcctgcg taacggtgag
    39601 cgggaagagg tggcgattaa cagcctgcgg cctggcgatg tgattgaagt cgccgcaggt
    39661 gggcgtttgc ctgccgacgg taaactgctc tcaccgtttg ccagttttga tgaaagcgcc
    39721 ctaaccggag aatccattcc ggtggagcgc gcgacgggcg ataaagttcc tgcaggagcc
    39781 accagcgtag accgtctggt aacgctggaa gtgctgtcag aaccgggtgc cagcgccatt
    39841 gaccggattc tgaaactgat cgaagaagcc gaagagcgcc gcgcccccat tgagcggttt
    39901 atcgaccgtt tcagccgtat ttatacgcca gcgattatgg ccgtcgccct gctggtaacg
    39961 ctagtgccgc cgctgctgtt tgccgccagc tggcaggagt ggatttataa aggtctgacg
    40021 ctgctgctga ttggttgccc gtgtgcgtta gttatctcaa cgcctgcggc gataacctcc
    40081 gggctggcag cagcagcgcg tcgtggggcg ttaattaaag gcggtgcggc gctggaacaa
    40141 ctaggtcgtg ttacccaagt ggcgtttgat aaaaccggta cgctgaccgt cggtaaaccg
    40201 cgcgttaccg cgattcatcc ggcaacgggt attagtgaat ctgaactgct gacactggcg
    40261 gcggcggtcg agcaaggcgc gacgcatcca ctggcgcaag ccatcgtacg cgaagcacag
    40321 gttgctgaac tcgccattcc caccgccgaa tcacagcggg cactggtcgg gtctggcatt
    40381 gaagcgcagg ttaatggtga gcgcgtgttg atatgcgctg ccgggaaaca tcccgctgat
    40441 gcatttgctg gtttgattaa tgaactggaa agcgccgggc aaacggttgt gctggtagta
    40501 cgtaatgatg acgtgctggg tatcattgca ttgcaggata ccctgcgcgc cgacgctaca
    40561 actgccatca gtgaactgaa cgcgctgggg gttaaaggag tgatcctcac cggcgataat
    40621 ccacgcgcag cggcggcaat tgccggggag ctggggctgg agtttaaagc gggcctattg
    40681 ccggaagata aagtcaaagc ggtgaccaag ctgaatcaac atgcgcccct ggcgatggtc
    40741 ggtgatggta ttaacgacgc gccagcgatg aaagctgccg ccatcgggat tgctatgggt
    40801 agcggcacag acgtggcgct ggaaaccgcc gacgcagcat taacccataa ccacctgcgc
    40861 ggcctggtgc aaatgattga actggcacgc gccactcacg ccaatatccg ccagaacatc
    40921 accattgcgc tgggactgaa agggatcttc ctcgtcacca cgcttttagg gatgaccggg
    40981 ttgtggctgg cagtgctggc agatacgggt gcgacggtgc tggtgacggc gaatgcgtta
    41041 aggttgttgc gcaggagata aggcaaaccg atcgcaacat tgagcgcgat cggtcccctc
    41101 gcccctctgg ggagagggtt agggggtgag gaaaaggcgg catcgaagcc aatcagcccc
    41161 tatcaaccgc ctttacgaat caaataacga taaggcagtc catccgtctc tttcgcaacc
    41221 agttcgtgtt ccataaaggt acaaaaccca ggaatatcgc gggtagtggc cggatcgtcg
    41281 gcgataatca gcaacgtttc gccaggctgc atattgcgca cggttttgcg caccatcatc
    41341 accggttccg ggcagcgcag gccaagcgcg tcgagtgtgt ggtcagggct ggaaaagaga
    41401 tcggtcattt tcttctcatc acttaaaaaa acggcgctag tttacgccct gtgagtccgt
    41461 aagcaaacca ggttaacgat tgcgtgaaaa ttaaccattg cattgtcaac gtaaagcagt
    41521 atcatgcggc ggctcgaaaa aagggtaagc acgttattat gttaaggtaa cagacgtgtc
    41581 gtacgtattg ggttccctca ccccaatggt taatcaaaaa ggtacaatat gaacgttttc
    41641 tcgcaaactc aacgctataa ggcgttgttc tggttatcgt tatttcatct tctggtgatc
    41701 acctccagta actatctggt tcagcttccc gtctccattt ttggtttcca taccacctgg
    41761 ggcgcgttta gctttccgtt tatttttctt gctaccgacc tgaccgtgcg tatttttggc
    41821 gcaccgctgg cccgacgcat tatcttcgcg gtaatgatcc ctgcgttatt aatctcctac
    41881 gtcatctcgt cgctattcta tatgggttcc tggcagggat tcggcgcact cgcccacttc
    41941 aacctgtttg tcgcccgtat cgccaccgcc agtttcatgg cctacgcgct ggggcaaatc
    42001 ctcgacgtgc acgtttttaa ccgcctgcgt cagagtcgcc gctggtggct ggcaccgaca
    42061 gcgtccacac tgttcggtaa cgtcagcgac acgctggcct ttttcttcat tgccttctgg
    42121 cgtagcccgg atgcctttat ggctgaacac tggatggaaa tcgcgctggt cgattactgt
    42181 ttcaaagtgt taatcagtat cgttttcttc ctgccaatgt atggcgtatt actcaatatg
    42241 ctgttgaaaa gactggcaga taaatccgaa atcaacgctt tgcaggcgag ttaaaggttc
    42301 gttatcagag ttgtgataag atggatgaat gagccgttat ggccgtttat cgaaaggaag
    42361 aagtcaatgc gcaatctggt taaatatgtc ggaattggcc tgctggttat ggggcttgcg
    42421 gcctgtgatg ataaagacac taacgctacg gcgcagggtt cggtcgcgga aagtaacgct
    42481 accgggaatc ccgtcaacct gcttgatggc aagttaagtt tctcgctgcc agcggatatg
    42541 accgaccaga gcggtaagct gggaacgcag gccaataaca tgcatgtctg gtccgacgcc
    42601 accgggcaga aagcagtcat cgtcatcatg ggcgatgatc cgaaagaaga tctggcggtg
    42661 ttggcgaagc gtctggaaga tcagcaacgt agccgcgatc cgcagctgca agtggtaacc
    42721 aataaagcca ttgagctgaa aggtcacaaa atgcagcagt tagacagtat tatctccgcg
    42781 aaaggccaga cggcgtactc ttccgttatt ctgggtaacg tgggtaatca actgctgacc
    42841 atgcaaatta cgctgccagc tgacgatcag caaaaagcgc aaaccaccgc agaaaacatc
    42901 attaatacgc tggttattca gtaagtttta agatgatgag gcggcctcag ggacgtgttc
    42961 cggaggccgt ttttttaatc gccacgtcag taataacgcg attgcgacca gtcccgccgc
    43021 cgccagataa atcaccggta cgcccgccca gctcatcacc agcccagcca gtggtccagt
    43081 cacgccaagc gataaatcca taaatacggt gtaagttgcc agcgccgccc cctaattttg
    43141 ctgcggaacc gcttttaccg ccactacacc caatgccggg aacaccagcg aaaacccggc
    43201 ccccgccagt aagacgccga ttttcgccat ccacggcata gtcgccacgc caaccagtag
    43261 caggccgatt atctcaacgc taaagcaaat catcgctacg tttaagccac cgatacggtt
    43321 aatgccgtta gggaataaca aacgcgtacc gacaaacgca cagctaaaca gcgtcagcgc
    43381 gaaagccgca ccgtcccaac ctttagcgtc ataaaacagc gtgataaagg tggcgatgac
    43441 gccaaatccg gcggaagcca gtgccagcgc cataccgtac agccagacgc gcccaagcac
    43501 cgcgcgaaac ggcagcggtt tgcctttact ggcttttacc gtcggacgcg ggatcgccaa
    43561 caaaatggcc accagcgcca cgcccataat gattaacgct aacgcctgca agccgcccca
    43621 gtgataaaac acgacgccta acggcgcacc catcgccatc gccccgtaag tgacaatgcc
    43681 gttccacgaa atcacccgcc cgatatgcag cgagccaacc actccaacgc cccatagggt
    43741 cgatcccgtt ccggcaaaac tttgcccaat cccaaggatg acgcgcccca ggcaaagtaa
    43801 taacaggctg atgacaggca gactggcggt taatcctgcc gtcagatacc ccagaccgct
    43861 caaaaagcag ccgcataaac cgaagacgac aatctttttg ggtcccagca aatcggcgta
    43921 acgtccggca tgagggcggc tcagcaaggt ggcgaaatat tgcaggctga taaccaatcc
    43981 tgcccagaag gcgctaaagc ccatcacatc atggacatag cccggtaata cagcgagcgg
    44041 caacccgatg gtgaggtagc tggcgaagtt aaacatgaca atggagacaa tgcgcaaatt
    44101 caggcgcaat ccgtttagcg cgggttcggc tacgggttcg ggcatgagga tcaccacatt
    44161 tttacaacag tgtttcattt ttaccatgtg ctgacgtgaa aatcagcagt aagaatcaga
    44221 atattgctgg cgtgactccc gctacactta acacaaaaag tcacaaggaa gccccaatgg
    44281 aaacccctca acccgataaa acgggcatgc acattctgct caagttggcc tcgctggtag
    44341 tgatcctcgc gggcattcac gcagcggcag atatcattgt gcagctgtta ctggcgctgt
    44401 tttttgccat cgtcctcaac ccgctcgtta cctggtttat tcgtcgggga gtacaacgcc
    44461 ccgttgccat tacgattgta gtggtggtga tgctgatcgc actaaccgcg ctggtcggcg
    44521 tactggcggc atcgtttaac gaatttatct ctatgctgcc gaagtttaat aaggagctga
    44581 cgcgcaaact ttttaaattg caggagatgt tgccttttct ttatttgcat atgtcgccgg
    44641 agcgaatgct gcagcggatg gactcggaaa aagtggttac cttcaccaca gcgctaatga
    44701 ccgggctttc cggggcaatg gcgagcgtgc ttttgctggt gatgaccgta gtttttatgc
    44761 tgtttgaagt gcgccacgtc ccttacaaaa tgcgttttgc gctgaataac ccacagattc
    44821 acatcgcagg attacaccgc gcacttaaag gcgtttcgca ttatctggcg ttaaagacgc
    44881 tactcagttt atggacaggt gtcatcgtct ggctggggct ggcgctaatg ggcgtacagt
    44941 ttgcgctgat gtgggcagta ctggcgtttt tgctcaacta cgtgcccaat atcggcgcgg
    45001 taatttccgc cgtaccgcca atgattcagg tgctgctgtt taatggtgtt tacgaatgta
    45061 ttctggtcgg cgcattgttt ttagtggtcc atatggtcat cggcaatatt ttagaaccac
    45121 ggatgatggg ccatcgcctg gggatgtcca ccatggtggt atttctttca ttgttaatct
    45181 ggggatggct gctcggcccg gtagggatgc tactttcggt gccattaacc agagtgtgta
    45241 aaatctggat ggaaacgacg aaaggtggca gcaaactggc gattttactg gggccggaca
    45301 gaccgaaaag tcggttaccg ggatgaggcg acaagtgata cgatacgcac tttcattttc
    45361 cattaaacgt tggccctgat atgtatcgga tagttctggg gaaagtttcg accttaagcg
    45421 cagctccact gccaccgggt ttacgcgagc aagcaccgca aggtccacga cgcgaacgct
    45481 ggctggcggg gcgtgcattg ctttcgcaca cgctttcccc gctaccggag atcatctatg
    45541 gcgaacaagg caaacctgca ttcgcgccgg aaacgccgct atggttcaac ttaagccata
    45601 gcggtgacga tatcgctctg ctgttgagcg atgaaggcga agtcggctgc gatatcgaag
    45661 tgattcgccc gcgcgccaac tggcgctggc tggcgaacgc cgtgttcagc ctcggggaac
    45721 acgctgaaat ggacgccgtg catcctgatc agcaactgga aatgttctgg cgcatctgga
    45781 cgcgcaaaga agccatcgtt aaacagcgtg gcggcagcgc ctggcaaatc gtcagcgtag
    45841 atagcaccta tcactcctcg ctgtcagtca gccattgtca gctagaaaat ttaagccttg
    45901 cgatctgcac ccccaccccc tttacgctca ccgccgacag tgtgcaatgg atcgattcag
    45961 ttaactgatc cgcccacccg actgcccatc tattgatcca gaacaggtaa tcagtatgac
    46021 gaatacttaa aatcgtcata cttatttccg ccatctattt taatccattg gggttaccat
    46081 gctctccaca ctccgccgca ctctatttgc gctgctggct tgtgcgtctt ttatcgtcca
    46141 tgccgctgca ccagatgaaa tcaccaccgc ctggccggtg aatgtcgggc cactaaaccc
    46201 gcacctttac acgcctaacc agatgttcgc ccagagcatg gtttatgaac cattggtgaa
    46261 atatcaggca gacggttcgg tgatcccgtg gctggcaaaa agctggactc attcagaaga
    46321 tggtaaaacc tggaccttca ccctgcgtga tgacgtgaaa ttctccaacg gtgaaccgtt
    46381 cgatgccgag gcggcggcag aaaacttccg cgcagtgctc gataaccgtc aacgtcacgc
    46441 ctggctggag ctggcaaacc agattgttga tgttaaagca ctcaataaaa cagagctgca
    46501 aattaccctg aaaagcgcct actatccttt cctgcaagaa ctggccctgc cccgtccttt
    46561 ccgttttatc gctccctcgc agtttaaaaa ccatgaaacc atgaacggaa ttaaagcgcc
    46621 gattggcacc ggaccgtgga ttttgcagga atcgaaactg aatcagtacg atgtcttcgt
    46681 ccgtaacgaa aactactggg gcgaaaagcc agcgattaaa aagatcacct ttaacgtcat
    46741 cccggacccg actacccgcg cggtggcgtt tgaaactggc gatatcgacc tgctgtacgg
    46801 aaacgaaggg ttattaccgc tcgatacctt cgcccgcttt agccaaaatc cggcttacca
    46861 cacccaactg tcacagccgc tcgaaaccgt gatgctggcg ctcaataccg ccaaagcccc
    46921 caccaacgag ctggcagtac gtgaagctct taattacgcg gtaaacaaaa aatcgctgat
    46981 tgataacgcg ttatatggca cccagcaggt cgccgacacc ctgtttgccc cttctgtgcc
    47041 ttacgctaat ctcggcctga aaccgcgcca gtacgatccg cagaaagcga aagagttgct
    47101 ggaaaaagcc ggttggacgc tgcctgcggg caaagacatc cgcgagaaaa atggtcagcc
    47161 gctgcgcatt gaactgtcgt tcatcggcac cgatgcgtta agcaaatcga tggcggaaat
    47221 cattcaggct gatatgcgcc agattggcgc agatgtctcg ctgattggcg aagaagagag
    47281 cagtatctat gctcgtcagc gcgacggtcg ttttggcatg attttccacc gcacctgggg
    47341 cgcgccatac gatccacacg ccttcctgag ttcaatgcgc gtaccgtcgc acgctgactt
    47401 ccaggcacag caaggattag ccgacaagcc gctgattgat aaagagatcg gcgaagtgct
    47461 ggcgacccat gacgaaacgc aacgtcaggc gctgtatcgc gacattctga cccgtctgca
    47521 tgacgaggcg gtttatctgc ctatcagcta catctcgatg atggtggtat caaaaccgga
    47581 gctgggtaat atcccctacg cgccgatcgc caccgaaatt ccgttcgaac agattaaacc
    47641 ggtgaaacct taatgttgcg ttacgtatta cgccgctttc tgctgctgat cccgatggtg
    47701 ctcgccgcct cggtgatcat ttttctgatg ctgcgcctcg gtaccggcga cccggcgctc
    47761 gattatctgc gtttatctaa cctgccgccg acgccggaga tgctggcctc tacccgcacc
    47821 atgctcgggc tggatcagcc gctgtacgtc cagtacggta cctggttgtg gaaggcgctg
    47881 catcttgact ttggtatctc attcgccagc caacgcccgg tactggacga tatgctgaac
    47941 ttcctgccca ccacgctgga acttgcaggt gcggcgctgg tattaattct gctcacttcc
    48001 gtaccgctcg gtatctgggc ggcgcgccac cgcgatcgtt tgccggattt cgccgtgcgc
    48061 tttatcgcgt ttcttggcgt gtcgatgccc aacttctggc tggcattttt gctagtgatg
    48121 gcgttttcgg tgtatctgca atggctaccc gcgatgggct acggcagctg gcagcacatc
    48181 attttacctg cggtttccat cgcctttatg tcgctggcga ttaacgcgcg actgctgcgc
    48241 gccagtatgc tggacgtcgc cggtcagcgt cacgtcacct gggcgcggct acgcggcctg
    48301 aatgacaaac agaccgaacg ccgccatatt ctgcgcaatg cctcgctgcc gatgatcacc
    48361 gccgtgggga tgcacattgg cgaattgatt ggcgggacga tgattatcga aaacatcttt
    48421 gcctggcctg gcgtcgggcg ctatgcggtg tcggcgattt ttaaccgtga ctatccggtg
    48481 attcagtgct ttacgctgat gatggtggtg gtttttgtgg tctgtaattt gattgtcgat
    48541 ttgctcaacg ccgcgctgga cccgcgcatt cgtcgtcatg aaggagcgca cgcgtgaact
    48601 ttttcctctc ttcccgctgg tcggtacgcc tggcgctgat cattatcgcc ctgctggcgc
    48661 tgattgcgct caccagccag tggtggctgc cgtacgatcc acaggcaatt gatttgccgt
    48721 cgtgcctgct ttcgccggat gcgcagcact ggctgggcac cgatcactta ggtcgcgata
    48781 ttttctcgcg gctgatggca gcgacccgcg tgtcgctcgg ttcggtaatg gcctgcctgc
    48841 tgctggtgct gacattaggg ctggttattg gcggcagcgc cgggttgatt ggcgggcgcg
    48901 ttgatcaggc caccatgcgc gtcgccgata tgtttatgac cttcccgacc tcgattctgt
    48961 cgttctttat ggttggcgtg ctcggcaccg ggctgaccaa cgtaattatc gccatcgccc
    49021 tgtcgcactg ggcgtggtat gcacgcatgg tgcgcagctt ggtgatttca ctacgccaac
    49081 gcgagtttgt gctggcgtca cggctttccg gtgcgggcca tgtgcgggtg tttgtcgatc
    49141 atctggcagg cgcggtgatc ccttcgctgc tggtgctggc aacgctggat atcggccata
    49201 tgatgctgca cgtcgcgggg atgtctttcc ttggcctcgg tgtgaccgcg ccgaccgccg
    49261 aatggggcgt gatgattaac gacgcgcgcc agtatatctg gacccagccg ctgcaaatgt
    49321 tctggccggg gctggcgctg tttatcagcg tgatggcctt taacctggtg ggtgacgcac
    49381 tgcgcgatca tctggatcct catctggtga cggagcacgc acactaatgc cgcaacagat
    49441 tgaactacgt aatatcgcgc tacaggccgc gcagccgctg gtacacggtg tatcgttaac
    49501 cctgcaacgc gggcgcgtgc tggcgttagt cggcggtagc ggcagcggga aatcattaac
    49561 ctgcgccgcg acgctgggca ttttgcccgc tggcgttcgc cagacggcgg gggaaatttt
    49621 agccgatggc aaaccggttt cgccttacgc cctgcgcggc atcaaaattg ccaccatcat
    49681 gcagaacccg cgcagcgcct ttaatccact gcacaccatg cacacccacg cgcgggaaac
    49741 ctgcctggcg ttagggaaac ccgccgatga cgctacgctt accgctgcca tagaagcggt
    49801 ggggctggaa aacgccgcgc gcgtgctgaa gctgtacccg ttcgagatga gcggcggcat
    49861 gttgcagcgc atgatgattg cgatggcggt gctgtgtgaa tcaccgttta tcatcgccga
    49921 tgaaccgacc accgacctcg acgtggtagc acaggcgcgc atcctcgatc tgctggaaag
    49981 cattatgcaa aaacaagcgc cgggaatgct gctggtcacc catgatatgg gcgttgtggc
    50041 gcgtctggcg gatgacgtgg cggtgatgtc acaaggtaaa attgtcgaac agggcgatgt
    50101 agaaacgctg tttaacgccc ccaaacatac ggtgacgcgc agcctggttt ccgctcatct
    50161 cgccctctac ggtatggagc tggcatcatg accttactta acgtctgcgg cctttcccat
    50221 cactatgccc acggtggatt tagcggaaaa catcaacatc aggcggtgct gaataacgtt
    50281 tccctgaccc tgaaaagcgg cgaaaccgtc gccctgctgg gacgcagcgg ctgcggaaaa
    50341 agcaccctcg cgcggctgct ggtaggctta gagtctccct cacaagggaa tattagctgg
    50401 cgcggcgaat cgctggcgaa acttaatcgc gcccaacgta aagccttccg ccgtgatatt
    50461 cagatggtgt ttcaggactc catcagtgcc gtgaatccgc gcaaaaccgt gcgcgagatc
    50521 ctgcgtgaac cgatgcgcca cctgctatca ctgaaaaaat ccgaacaact ggcgcgcgcc
    50581 agcgaaatgc tgaaggcggt cgatctcgat gacagcgttc tcgacaaacg cccaccgcag
    50641 ttaagcggcg gccagctcca gctcgtctgc ctggcacgcg cgctggcggt cgaaccgaaa
    50701 ttgctgattc tggatgaagc cgtttctaac cttgatctcg tgttacaggc gggcgtcatt
    50761 cgcctgctga aaaatctaca acaacagttt ggcactgcct gcctgttcat cactcatgac
    50821 ttacgcctgg tggaacgctt ttgccagcga gtgatggtga tggacaacgg acaaatcgcc
    50881 gaaacccagg cggtgggaga taaattaacc ttttcctctg acgccggacg tgtgctacaa
    50941 aacgcggtat tacccgcatt ccccgtgcgc cgtcgcacct cagaaaaggt ttaacgccaa
    51001 tgcaacgagt caccatcacg cttgatgacg atttactgga gacgctggac agcctgagcc
    51061 agcgtcgtgg ttataacaac cgttccgaag ctatccgcga tattctgcgt agtgccctgg
    51121 cgcaggaagc tacccagcag cacggcacac aaggtttcgc ggtgctgtcg tatgtgtatg
    51181 aacacgaaaa acgcgactta gccagccgta ttgtttccac ccagcatcat caccacgacc
    51241 tctccgtcgc cacgctacat gtgcacatca accacgacga ctgtctggaa atcgccgtgt
    51301 tgaaaggtga catgggtgac gtgcagcatt ttgccgatga cgttatcgcc cagcgcggcg
    51361 tgcggcacgg gcatttgcag tgcctgccga aggaagattg agttatttct aaaacaagga
    51421 aagaaaagat attaactgaa cttaatttat aaaattaagt tcagttgcat agatagatcc
    51481 tgtaaaggca gatattctgc gtaatgtacg tcgctaaaat ttataaatga ttcaaacgct
    51541 cattcagtgt ttcaataata taggtattaa cagagacttg ttgctgcgca gccgcattat
    51601 taaggcgttc actcagcgat tctggatagc gtaacgtaaa ggtcttaatc ttttctgtac
    51661 gcgcatacgg ctcaataccc gcagctttac aatcctcaag atactctcgc agggaaagct
    51721 ctccttcttt ttgcagcccc tgaatactgt cagagacaaa atcacaataa cctgacaagc
    51781 ccaggaattt gccacgaaat gcattcagtt ctggcacgta ggtaataaca gctggttgcc
    51841 cggcaatctc catagagttt ggcgtcttca gtttgatcat ggttttaccc ctatacttag
    51901 taaccagtca cgcacgcttt ctacagcgcc tttatcggta tctggcgacg gatgaggccg
    51961 atggaaacac gcaacgctca tatttagtat gaacttgcaa cgcgaacctc ttccttcctt
    52021 aatttctccg cctaatgctt taaccagtga ctctatatca gaccacttga tcccttgagg
    52081 aacaggtgtt ttaaatattt gatccaatgt atttttttgt ttcttccgta aagcctgtac
    52141 cttggttctc atggcatgtc cttatgactt cactttatga catcacagta tgaagtcaag
    52201 tttcccttcc ttacgccatt gtcccaatcg tcttcctgaa tcgcagcagt gcgatggtga
    52261 aaaacgcgcc gccaattgcc atcagcgtca ggaactgcgg ccagacgatt tcgaatccgg
    52321 caccccggta gaggatggcc tgcgcgaggc taacaaagtg tgtcgtcggc atggtcagca
    52381 taatgtcctg caccatctgc ggcatacttt cgcgcggcgt ggatccaccg gaaagcattt
    52441 gcagcggcag cagcaccaga atcaccagca gccccagttg cggcattgaa cgcgctatcg
    52501 tccccataaa aatgccgatt gacgtggtgg caaacagact gagcgccacg cccagcataa
    52561 acagcgggat cgagccttca atcggtacgc ccagtacacc tttcaccatc agcaccagcg
    52621 ataatcccga taccaccagc accaccagcc ccatcgacca gatcttcgcc atcatgatct
    52681 caaacggcgt tatcggcatc accagtaaat gttccaccgt gccgtgttca cgctcgcgga
    52741 tcagcgccga tccggtcaat acaatcgcca gcatggtaat gttgttgatg atcgccatca
    52801 ccccgccaaa ccacgcggga tcaaggttcg ggttaaagcg catccgggtt tccagcgata
    52861 ccaacggttc gctgttatca cggtagcgcg cgacaaagct gttcacttca ccgttgataa
    52921 tattctggat atacccattg ccggtaaatg cctggctcat gcgcgtggca tcgacgttca
    52981 cctgaatatc cggctggcgt ccggcgagga catcacgctg aaaattaggc ggaatattta
    53041 tcgcgaaggt atagcgtccg gcgtccagtc cggcatccat ctcatcggcg gtgatcatct
    53101 ccggtggcaa aaaccacgga cgatagaagc tgttaacgat ccggttcgat aactgcgatt
    53161 gatccatatc ggcaatggcg atcggcgcga ggttcaacga tcctggcgta acggtcgctg
    53221 acgaatacac cgacaccgta aacgagaaga caatcagcgt cagcatcgct ttatcaccga
    53281 gcagactgcg caactctttg atacccagat taaaaatatt gcgtaaatgg cgcatcatcc
    53341 ctcctgtttt ttcagcagca ggatgcttaa gcccatcacc agcgggatgg ctatcagcaa
    53401 cgggataaaa agttgccaca aatcagtcag atccagcgct ttcgagaacg ttccgcgggc
    53461 gatagtcaga aaatgactgg tcgggtaaac ctcgccgatc caacgtccag gcccttccag
    53521 cgaagctacc ggatcgatca tcccggaaaa ctgtgtcgcc gggatcaatg tgataatcgc
    53581 cgttccgaaa atggcggcaa tctggctttt cataaaggtg gagatcagca gccccattcc
    53641 agtggcaatg atgatataca gcagcgccgc caaggtgagc gtcaggaaac tgcctttatg
    53701 cggtacgcca aacacaaaca ccgacaggcc gcagagcagg aaaaagttca gcatccccag
    53761 cgcgatgtac ggcagctgtt tgccaagcaa aaattcgcta cgggtggtgg gggtcacgta
    53821 aaggttgata atcgacccta gctctttttc ccgcaccacg ctaagggcgc ttagcattga
    53881 cgggatcatc atcagcagaa gcgggatcac cgccggaaca atcgctggca ggctttttac
    53941 gtccgggtta tagcgataac gcgtctcaat gttcatcagc ccgctttggc tggcgggtgt
    54001 caattgtcgg ctcgccacat cctgaagcca actctggtgc attgcctgca cgtaaccttt
    54061 taccgtttcg gcacgactgg gcatcgctcc gtcgatccag acgccgagtt ccacaggcgt
    54121 accacgcgcg atatcgcgcc cgaaattggg cgggatctca atggccaccg tgatatcgcc
    54181 cgcacgcatc cgacgatcaa gctcgtcata actggtgagc ggcggctgtt cgataaagta
    54241 acgggaaccg gagaggttga gtgtccacgc ctggctactg acggtctggt cgcggtcgag
    54301 caccgcaaag cgcaggtttt ccacatccat actgatgccg taacccatta tcagcatcag
    54361 gatcaccgtt cccatcagcg ccagcgtcga acgtactgga tcgcgtcgca gttccagcgc
    54421 ttcgcggcgg ctgtagctaa acagacggcg caggctaaat ccctgacgcg gcgcgtgggt
    54481 ggtgtcgtgt accacgggcg gcgcttcggc ttcgttgctc tgccctgccg cttcctgcaa
    54541 ataggcgata aatgcctctt ccagactggc ggctccgcgt ttctcaacca gttcctgcgg
    54601 tgtaccgctg gcaagcactt ttccggcgtg catcagtgag atgcggtcgc aacgttccgc
    54661 ttcgttcata aagtgggtgg agatgaagat agtcactttg tcctggcgcg agagatcgac
    54721 catcaactgc cagaacatat ccctcgccac cggatcgaca ccagaagtag gctcatcgag
    54781 gattaacatc tccgggcgat gaatcaccgc caccgccagc gaaagccgct ggcgaatgcc
    54841 gagcggcaat gactccggca gaacatcttc aacgtcgttg agcttaaaac gctcgctcat
    54901 ttcagccact cgcgcgggaa tttccgcttc cgggatgtga aacaaacggg catgtaactc
    54961 caggttttgc cgcacggtga gttcgttata gagcgaaaac gactgcgaca tatagcccac
    55021 acgacggcgg gtatcgatat cttttggatc aaccggttgc ccaaatagcc acgcctcacc
    55081 ttcgctggcg ggcagcagtc cggtaagcat tttcatggtg gtggatttac cgcagccgtt
    55141 cgaaccaagg aatccaaaaa tctccccgcg tggaatgcgg aaattgacgt gatcaacggc
    55201 aacgaaggaa ccaaaacgca tggtcagatc gcgcgcttcg atggcaatct ctgcgttttc
    55261 aggttgatac ggtgggatca ctaccgcctg atgcgcctgg cgttgcgctt gcggtaacag
    55321 attaataaat gcttcttcca gcgtggcgct ttgcgtttgc tgccgcagct cttcggcgct
    55381 gccggttgcc agcacttctc cggcattcat cgctaccagc cagtcgaagc gttcggcctc
    55441 ttccatatag gcggtggcga ccagcacgct catattgctc tgccgctggc gaatactgtc
    55501 gatcagatcc cagaactggg cgcgggagag cgggtcaacc cccgttgttg gctcatcaag
    55561 gatcaacagt tccgggtcgt ggattaacgc gcagcacagc ccaagttttt gcttcatccc
    55621 gccggagagt ttacctgccg gacgatcgcg aaacggtgct aacccggtgc tggtcagcag
    55681 ctcattaatt cgcacttccc gctccgcttt atcgtgaccg aacaggcgag cgaaaaaatc
    55741 gacattttca tacaccgaca aggtgtggta gaggtttttg cccagcccct gcggcatcca
    55801 ggcgatgcgc gggcagacgt ctcggcgatg cttcgggtcg tgcatatcgc cacccagcac
    55861 caccacattg ccctgctcaa tgacgcgggc accggaaatc agtgacaata aactcgactt
    55921 cccgacgcca tccggaccaa tcagcccgac catgcaacgg gccggaatat cgagagtgat
    55981 attgttcagc gcaacggttt ttccataatg ctggctcacg cccgccagtt gcgcgacagg
    56041 cgggacagga accagttcca gatgcgtcat tgcggcaacc tcaccacgag gtcgtcaggc
    56101 cacggaagtt cttcattcac ccgcacccac gctacgcccg gtaaaccggt tttgacatat
    56161 tccagatgct gctggagtaa ttccggtggg atacgcgctt tgacgcggaa catcagtttc
    56221 agccgttcat cgctggtttc gacggttttt ggcgtgaact gggcgacact ggcgacaaaa
    56281 ctgatggttg caggaatacg cagatctggc gcggcatcga ggatcagccg ggcttcaccg
    56341 cccagtttca gcgtgcccgc ctgttcggtt ggcaggaaga aggtcatata gacgtcgctg
    56401 agatcgacca tattcagcac ccgaccgcct gccgccagca cttcccctgg ctcggcaacc
    56461 cgatactgca cgcgtccgtc acgcggggct ttcagttcac tgtcatcgat atctgcggca
    56521 atgcgccgtt cagtggcttg cgctgcttcg acccgagttt gcgcctgaat gatattggtg
    56581 cgtgctgctt ctatagccgc tttagaagcc gaaacctggg ctttcgctga ttccagcgca
    56641 gctcgggcgc tctcagcggc ggcgcggtcg tcatccagct gttgcgcaga aatcgcccct
    56701 cgttgggcga gcgagcggga acgcgtatga cgtttggcaa cagagtccag ttcggcctgg
    56761 cgttgattga ccagcgactg tgcggcacga gtttcacttt gtcgttgctc cagcaaagcc
    56821 tgcgcggcgg caacggcgct ttgtgcctct ttgatttgcg cgatggcttc cagtcgctgt
    56881 tcctgcaaca cgcgagtatc catcttcgcc agcacttcgc cttcgcgaac aaactggcct
    56941 tctttcacca gaatggtgtc gatacgcccg gcagttttgc tggcgatatc cacttccgtc
    57001 gcttcaatgc gcccattgct gacagcaaag ccttccggca cacccgccgg gcgcaacagc
    57061 caccacgcca cgacggcagc caccgccagt gccccgacaa cccaccacgc cagatggcgc
    57121 ttactcttat ccataatcga cccgccataa tccctgtaag caaacgacgc gtgttacaac
    57181 tgccgccgaa cgacgttcag cggattcttc catccgggat aagaaggaaa cgacaaatac
    57241 gaccagcgcg cagagcattg agcgccaacg gggaaggctt agcgaaaacg ggtagtaatg
    57301 ttgtcatcat cagcattcct ggccgtaaat gaaacacgca tactcataag tgtgatctat
    57361 aaaactggct gacaaaacgg tatccatcct gttggatcgc gtttcacgcc tgttcagttg
    57421 caggaacaga gcattccttt ttacgtttta tcaacagtct caaccagata gtaacattaa
    57481 tattcatgtt gtctatggtt cagagctgta acattgtgcg ccttctaatc aaaggcataa
    57541 gtccatttct gtttttttca tgctggtaag acttccaggg agtggttatc tctggccctg
    57601 caccaaggtt aatttggcaa ttatgcatgc tgaacattct tccagcatgt ttactgtcac
    57661 gggcgttaag acctaaaact gtccccgttt cacctatgcg ttctctccca ccatttccgt
    57721 tatgtgcaac aacttgattt atacctaaca tttgtgaaca tgctaatagt gcttcaagtt
    57781 taggtctgta atctgtcgaa acagaaaaat aacgattaaa ggatcgacaa gagatagccc
    57841 ggctagtttt aaactttttg ccattaatta tagcatgatg ctttttatta ataatttccg
    57901 ctaattcgac cggatcgaga gcattggtca tttcatcaat agagacagtt atcggtccta
    57961 taatgtaacg ttttcgctga tcatcaaaag ttatcccatg atgatttgcc attattttgt
    58021 atttggggtc atagaaacat acaggataat cttttatccc gtcgtaggta tcctcatcaa
    58081 gtggtttcat gcgcgtgaag ttttgcatat agttaccatt gcaattcgtt tcatgatttc
    58141 cagctaatac atgaacattt tcattgatac tcattatgcg ttttaacaaa tcgatgataa
    58201 attgaacgcc acagatatta ctaaatcgat cgccggtttg gtcgccaaga aacgtacatg
    58261 gcgtgactgc gtctttatta ataaccaaat aaggctgaag actttctaaa acattatctc
    58321 ttctgttcag tatactgatt attgtattaa cattagtgtc aagttgctca ttaaagttct
    58381 cccttagcaa tacgttgaga acttcatctt ctatattaag caactggcta aggaaatttt
    58441 ctccttcagc catgattccc aaatgtcctg ttgcaaaaag cgcgtacaaa actgcaccga
    58501 ctgagctatc ggtatcgcca aaataggtcg gcctggtact tgtgggatca acaggtgaac
    58561 aattggagaa ataggttctg tcagcgaact gggattttat aattgtttcg aatttaatat
    58621 caaatacatt gtattgcgat atttcccagt tccagatggg tttgtacgac tcagtaactg
    58681 caacatcatt agcattatca tttactgtca tattattaag taaataaaca atgctgcacc
    58741 aattaatatc ggtattatca agagggcaac cgtgattttt actattaatt gtcacaataa
    58801 actgataaca gttattccta cgtagaatat gagcgcgcat gcccgatagc atactatatt
    58861 tttctgaatg attcgtttgt atggtaaatg aaattttgtc atcttgaata gattgttgga
    58921 aacgttctat ttgccaatca tcaatttttt ccacaacttc accgagcatt gtcctggcaa
    58981 tacttgatat atcatcacct ataccatttg caatataccc aaacagttct ttagtgaagt
    59041 tggtaacatg ctcttgcacc atatcatttg ttgcgattgt cgttgtactg ccattagtgc
    59101 ctgccacagt tccaattttc atcttttacc tcatattatt caccgcgagg aatacatagt
    59161 taacatttta tcttatgaca tacacctcat attcgatggg aaaataatta tattttgcac
    59221 aaatcattca gccttaaagc ataatatgaa atttcttatc tcatagcaga gaaagtccct
    59281 aaagattgta gaggcgtcat cagtacatac ctctacaatc gattaagaaa acctatgaaa
    59341 tactggcaaa attaaaaaga gataccacag cactcatgaa gttttattga ggttgaaaac
    59401 aactaagccc tgggacacct tttttatcac cacgttcgtt aggtgctatc ttccaagaat
    59461 ttagccccat ggtataatta tcaatttttt gatgctggtc tacccctaat ccataatggc
    59521 caa
//
LOCUS       DAETPZ010000015        59445 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.015, whole genome shotgun sequence.
ACCESSION   DAETPZ010000015 DAETPZ010000000
VERSION     DAETPZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.015"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            545..1834
                     /gene="sdaC"
                     /locus_tag="K0W59_001051"
     CDS             545..1834
                     /gene="sdaC"
                     /locus_tag="K0W59_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HBI3022186.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLSMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1892..3259
                     /gene="sdaB"
                     /locus_tag="K0W59_001052"
     CDS             1892..3259
                     /gene="sdaB"
                     /locus_tag="K0W59_001052"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HBI3022187.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDISIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3370..4125
                     /gene="xni"
                     /locus_tag="K0W59_001053"
     CDS             3370..4125
                     /gene="xni"
                     /locus_tag="K0W59_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HBI3022188.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTLAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4178..5326)
                     /gene="fucO"
                     /locus_tag="K0W59_001054"
     CDS             complement(4178..5326)
                     /gene="fucO"
                     /locus_tag="K0W59_001054"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBI3022189.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5354..6001)
                     /gene="fucA"
                     /locus_tag="K0W59_001055"
     CDS             complement(5354..6001)
                     /gene="fucA"
                     /locus_tag="K0W59_001055"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HBI3022190.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6548..7864
                     /gene="fucP"
                     /locus_tag="K0W59_001056"
     CDS             6548..7864
                     /gene="fucP"
                     /locus_tag="K0W59_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HBI3022191.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRITGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7897..9672
                     /gene="fucI"
                     /locus_tag="K0W59_001057"
     CDS             7897..9672
                     /gene="fucI"
                     /locus_tag="K0W59_001057"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HBI3022192.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHTCGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9781..11200
                     /gene="fucK"
                     /locus_tag="K0W59_001058"
                     /pseudo
     CDS             9781..11200
                     /gene="fucK"
                     /locus_tag="K0W59_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11202..11624
                     /gene="fucU"
                     /locus_tag="K0W59_001059"
     CDS             11202..11624
                     /gene="fucU"
                     /locus_tag="K0W59_001059"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HBI3022193.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11682..12413
                     /gene="fucR"
                     /locus_tag="K0W59_001060"
     CDS             11682..12413
                     /gene="fucR"
                     /locus_tag="K0W59_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HBI3022194.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGAPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGYLDEVTHIISDERQVATSLVTA"
     gene            complement(12457..13557)
                     /gene="rlmM"
                     /locus_tag="K0W59_001061"
     CDS             complement(12457..13557)
                     /gene="rlmM"
                     /locus_tag="K0W59_001061"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HBI3022195.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13550..13945)
                     /locus_tag="K0W59_001062"
     CDS             complement(13550..13945)
                     /locus_tag="K0W59_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HBI3022196.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13964..14881)
                     /gene="gcvA"
                     /locus_tag="K0W59_001063"
     CDS             complement(13964..14881)
                     /gene="gcvA"
                     /locus_tag="K0W59_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HBI3022197.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKIPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15232..15459)
                     /locus_tag="K0W59_001064"
     CDS             complement(15232..15459)
                     /locus_tag="K0W59_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HBI3022198.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15651..16856
                     /gene="csdA"
                     /locus_tag="K0W59_001065"
     CDS             15651..16856
                     /gene="csdA"
                     /locus_tag="K0W59_001065"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HBI3022199.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16856..17299
                     /gene="csdE"
                     /locus_tag="K0W59_001066"
     CDS             16856..17299
                     /gene="csdE"
                     /locus_tag="K0W59_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HBI3022200.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELELRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17350..18156)
                     /gene="tcdA"
                     /locus_tag="K0W59_001067"
     CDS             complement(17350..18156)
                     /gene="tcdA"
                     /locus_tag="K0W59_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HBI3022201.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18395..19492)
                     /gene="mltA"
                     /locus_tag="K0W59_001068"
     CDS             complement(18395..19492)
                     /gene="mltA"
                     /locus_tag="K0W59_001068"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HBI3022202.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGKTHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19700..19776
                     /locus_tag="K0W59_001069"
     tRNA            19700..19776
                     /locus_tag="K0W59_001069"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19734..19736,aa:Met,seq:cat)
     gene            19810..19886
                     /locus_tag="K0W59_001070"
     tRNA            19810..19886
                     /locus_tag="K0W59_001070"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19844..19846,aa:Met,seq:cat)
     gene            complement(19961..21214)
                     /gene="amiC"
                     /locus_tag="K0W59_001071"
     CDS             complement(19961..21214)
                     /gene="amiC"
                     /locus_tag="K0W59_001071"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HBI3022203.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21446..22777
                     /gene="argA"
                     /locus_tag="K0W59_001072"
     CDS             21446..22777
                     /gene="argA"
                     /locus_tag="K0W59_001072"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HBI3022204.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22839..24665)
                     /gene="recD"
                     /locus_tag="K0W59_001073"
     CDS             complement(22839..24665)
                     /gene="recD"
                     /locus_tag="K0W59_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HBI3022205.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMLL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24665..28207)
                     /gene="recB"
                     /locus_tag="K0W59_001074"
     CDS             complement(24665..28207)
                     /gene="recB"
                     /locus_tag="K0W59_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HBI3022206.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEVIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNDDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28200..31088)
                     /gene="ptrA"
                     /locus_tag="K0W59_001075"
     CDS             complement(28200..31088)
                     /gene="ptrA"
                     /locus_tag="K0W59_001075"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HBI3022207.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKTEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31264..34632)
                     /gene="recC"
                     /locus_tag="K0W59_001076"
     CDS             complement(31264..34632)
                     /gene="recC"
                     /locus_tag="K0W59_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HBI3022208.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPLWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34645..34968)
                     /locus_tag="K0W59_001077"
     CDS             complement(34645..34968)
                     /locus_tag="K0W59_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBI3022209.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(34953..35360)
                     /locus_tag="K0W59_001078"
     CDS             complement(34953..35360)
                     /locus_tag="K0W59_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HBI3022210.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35357..35920)
                     /locus_tag="K0W59_001079"
     CDS             complement(35357..35920)
                     /locus_tag="K0W59_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBI3022211.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35911..36381)
                     /locus_tag="K0W59_001080"
                     /pseudo
     CDS             complement(35911..36381)
                     /locus_tag="K0W59_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36565..37359)
                     /gene="thyA"
                     /locus_tag="K0W59_001081"
     CDS             complement(36565..37359)
                     /gene="thyA"
                     /locus_tag="K0W59_001081"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBI3022212.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(37366..38241)
                     /gene="lgt"
                     /locus_tag="K0W59_001082"
     CDS             complement(37366..38241)
                     /gene="lgt"
                     /locus_tag="K0W59_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBI3022213.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38392..40638)
                     /gene="ptsP"
                     /locus_tag="K0W59_001083"
     CDS             complement(38392..40638)
                     /gene="ptsP"
                     /locus_tag="K0W59_001083"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBI3022214.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSI
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40651..41181)
                     /gene="rppH"
                     /locus_tag="K0W59_001084"
     CDS             complement(40651..41181)
                     /gene="rppH"
                     /locus_tag="K0W59_001084"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HBI3022215.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41866..42555
                     /gene="mutH"
                     /locus_tag="K0W59_001085"
     CDS             41866..42555
                     /gene="mutH"
                     /locus_tag="K0W59_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HBI3022216.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42624..43337
                     /locus_tag="K0W59_001086"
     CDS             42624..43337
                     /locus_tag="K0W59_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBI3022217.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43475..43693
                     /gene="ygdR"
                     /locus_tag="K0W59_001087"
     CDS             43475..43693
                     /gene="ygdR"
                     /locus_tag="K0W59_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HBI3022218.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43801..44841
                     /locus_tag="K0W59_001088"
     CDS             43801..44841
                     /locus_tag="K0W59_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HBI3022219.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44874..46067)
                     /gene="lplT"
                     /locus_tag="K0W59_001089"
     CDS             complement(44874..46067)
                     /gene="lplT"
                     /locus_tag="K0W59_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HBI3022220.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FCPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACVLAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(46060..48219)
                     /gene="aas"
                     /locus_tag="K0W59_001090"
     CDS             complement(46060..48219)
                     /gene="aas"
                     /locus_tag="K0W59_001090"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HBI3022221.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSVITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48608..49639
                     /gene="galR"
                     /locus_tag="K0W59_001091"
     CDS             48608..49639
                     /gene="galR"
                     /locus_tag="K0W59_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HBI3022222.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDENGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49646..50908)
                     /gene="lysA"
                     /locus_tag="K0W59_001092"
     CDS             complement(49646..50908)
                     /gene="lysA"
                     /locus_tag="K0W59_001092"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBI3022223.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51030..51965
                     /gene="lysR"
                     /locus_tag="K0W59_001093"
     CDS             51030..51965
                     /gene="lysR"
                     /locus_tag="K0W59_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HBI3022224.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(51952..52644)
                     /gene="ygeA"
                     /locus_tag="K0W59_001094"
     CDS             complement(51952..52644)
                     /gene="ygeA"
                     /locus_tag="K0W59_001094"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBI3022225.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGKFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52773..54186)
                     /gene="araE"
                     /locus_tag="K0W59_001095"
                     /pseudo
     CDS             complement(52773..54186)
                     /gene="araE"
                     /locus_tag="K0W59_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
     gene            complement(54501..55262)
                     /gene="kduD"
                     /locus_tag="K0W59_001096"
     CDS             complement(54501..55262)
                     /gene="kduD"
                     /locus_tag="K0W59_001096"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HBI3022226.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55292..56128)
                     /gene="kduI"
                     /locus_tag="K0W59_001097"
     CDS             complement(55292..56128)
                     /gene="kduI"
                     /locus_tag="K0W59_001097"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBI3022227.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVAVKD
                     LR"
     gene            complement(56448..57596)
                     /locus_tag="K0W59_001098"
     CDS             complement(56448..57596)
                     /locus_tag="K0W59_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBI3022228.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGG"
     gene            57851..59080
                     /locus_tag="K0W59_001099"
                     /pseudo
     CDS             57851..59080
                     /locus_tag="K0W59_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        14657 a        15972 c        14485 g        14331 t
ORIGIN      
        1 cggtgacgtt tgttgccggg ttttctcgtt tttggtcact tactcatcaa ctcatttcat
       61 ttgttatatg aatgtttctt accaccctca cgcgacaaat atcatcacag ttaatatgcc
      121 atacaattta tgttgcaacg caaacgtttc cctattttca taaaccgtat tttatcgcta
      181 tgaaaaagaa attatcgcca tgattaacta aaagtattga ttttttcagt tcaacctaca
      241 tatattgcgc gccccggaag aagtcagatg tcgtttaatg ggcaaatatt gcccttaaat
      301 tctcttttac ttttgattta cagagtaaag cgttgggata atctatcttc caagcagatt
      361 attgtatttg ggatcaagat cactgataga tacataactt gtgtgtatct ttccgccctc
      421 aaattattac agcggtaaat gattaagcca tcgccgatag acagatttca tttttacggt
      481 caggcacctt cccgggctga actggctaaa agctgaatta tttgcattcc tccaggagaa
      541 atagatggaa acgactcaaa ccagcacgat tgcgtcgaaa gactctcgta gtgcctggcg
      601 caagacagac accatgtgga tgctgggcct ttacggcacg gcaatcggcg cgggcgtgct
      661 gttcctgcca atcaacgccg gtgttggcgg tatgatcccg ctgatcatca tggctatcct
      721 tgcgttcccg atgacgtttt ttgctcaccg cggcctgact cgcttcgtac tgtctggtaa
      781 aaacccgggc gaagacatca ccgaggttgt agaagaacac tttggtattg gcgcaggtaa
      841 actgattacc ctgctctact tcttcgctat ctacccgatc ctgctggttt atagcgtggc
      901 aatcaccaat accgttgaaa gcttcatgtc tcaccagctg agtatgacgc caccgccgcg
      961 tgcgattctg tcgctgatcc tgatcgtggg tatgatgacc atcgttcgct ttggtgagca
     1021 gatgatcgtt aaagcgatga gtattctggt attcccgttt gttggcgtac tgatgctgct
     1081 ggctctgtac ctgatcccgc agtggaacgg cgcagcactg gaaacgctgt ctctggacac
     1141 tgcatctgca accggaaacg gtctgtggat gaccctgtgg ctggcaattc cggtaatggt
     1201 gttctcgttc aaccactctc cgatcatctc ttctttcgcc gttgcgaagc gtgaagagta
     1261 cggcgatatg gcagaacaga aatgctcgaa gatcctggca ttcgcacaca tcatgatggt
     1321 gctgaccgta atgttcttcg tcttcagctg cgtactgagc ctgactccgg cagacctggc
     1381 tgcggctaaa gagcagaaca tctcgattct gtcttacctg gctaaccact ttaacgcacc
     1441 ggttatcgcg tggatggctc cgattatcgc gattatcgct atcaccaaat ccttcctcgg
     1501 ccactacctg ggcgcacgtg aaggcttcaa cggtatggtg attaaatctc tgcgtggtaa
     1561 aggtaagtct atcgaaatca acaagctgaa ccgtatcact gcgctgttca tgctggtaac
     1621 gacctggatt gttgccaccc tgaacccgag catcctgggt atgattgaaa ccctgggcgg
     1681 cccaatcatc gcgatgatcc tgttcctgat gccgatgtac gcaattcaga aagtaccggc
     1741 aatgcgtaag tacagcggtc acatcagcaa cgtattcgtt gtcgtgatgg ggctgattgc
     1801 aatctccgca atcttctact ctctgttcag ctaagtcctt tcgcgccgct ttcgggcggc
     1861 gcttcctccg ttttaacgcg atgtatttcc tatgattagc gtattcgata ttttcaaaat
     1921 cggcattggc ccttccagtt ctcataccgt tggaccaatg aaagcgggta aacaatttac
     1981 cgacgatctg attgcccgta acctgcttaa agacgtgacc cgcgtggtgg ttgacgtgta
     2041 cggctcgctc tctctgactg gtaaaggcca ccacacagat atctccatta ttatgggcct
     2101 ggcgggtaac ctgccggata ccgtggatat cgattccatc cccggtttta ttcaggatgt
     2161 gaatactcat ggtcgcctga tgctggcaaa cggtcagcat gaagtggagt tcccggttga
     2221 tcagtgcatg aacttccatg cagacaacct ttctctgcat gaaaacggta tgcgcattac
     2281 cgcgctggcg ggcgataaag tcgtttacag ccagacttac tactctattg gcggtggctt
     2341 tatcgttgat gaagagcatt ttggccagca ggatagcgca ccggttgaag ttccttatcc
     2401 gtacagttca gcagccgatc tgcaaaaaca ttgtcaggaa accgggctgt cactctctgg
     2461 cctgatgatg aaaaacgagc tggcgctgca cagtaaagaa gagctggaac agcacctggc
     2521 gaacgtctgg gaagtcatgc gtggcggtat tgagcgcggt atttccaccg aaggcgtgtt
     2581 gcctggcaaa ctgcgcgttc cacgccgtgc tgcggcacta cgccggatgc tggtcagcca
     2641 ggataaaacc accactgacc cgatggcggt tgttgactgg atcaacatgt ttgcactggc
     2701 agtgaacgaa gagaacgctg ctggcggtcg cgtggtgact gcgccgacta acggtgcgtg
     2761 cgggattatc ccggctgtgc tggcgtacta cgataagttt atccgcgaag tgaacgctaa
     2821 ctcactggct cgttacctgc tggtagccag cgccattggt tctctttata agatgaacgc
     2881 gtcgatttct ggtgccgaag tgggttgcca gggtgaagtt ggcgtggcgt gctctatggc
     2941 ggcagctggt ctggcagaac tgttaggcgc aagcccggcg caggtttgca tcgcagcgga
     3001 aatcgcgatg gagcacaacc tcggtctgac ttgtgacccg gtcgcggggc aggttcaggt
     3061 accgtgcatt gagcgtaacg ccattgcagc agtgaaagcg gtgaacgccg cgcgtatggc
     3121 actgcgccgt accagcgaac cgcgcgtctg cctcgataaa gttatcgaaa ccatgtacga
     3181 aacaggtaaa gacatgaacg ccaagtaccg cgaaacctct cgcggcggcc tggcgatgaa
     3241 gatcgttgcc tgcgattaat ctctcccaga ggcctcgttt tgcgaagcct cttcctgttt
     3301 tctcaaccag ccatagcctg ttccggcatc gaatgttacc cttatcgcct gatctttaag
     3361 ggggttatcg tggctgttca tttgcttatt gtcgatgcac tgaatcttat ccgtcgcatt
     3421 catgccgttc agggttcgcc ctgtgtcgaa acctgccagc atgcgctcga tcagctcatt
     3481 atgcacagcc agccaaccca cgcggtcgcc gtttttgatg atgaaaaccg cagtagcggc
     3541 tggcgtcatc agcgtttacc agattacaaa gcggatcgac cgccaatgcc ggaagagttg
     3601 cacgacgaga tgcctgcatt acgcgccgcc tttgagcaac gcggcgtccc gtgctggtca
     3661 gccagcggca acgaagccga tgacttagcc gccacactgg cggtcaaagt gacactggcc
     3721 gggcatcagg caacgattgt ttcgacagat aaaggctact gtcagttact ttcaccgaca
     3781 ttacgtattc gtgattactt ccagaaacgt tggctggatg cgccatttat cgataaagaa
     3841 tttggcgttc aaccgcagca gttgcccgat tactggggac ttgcggggat cagcagttca
     3901 aaggtaccgg gtgttgcggg aatcggacca aaaagcgcca cgcagctgct ggtcgagttt
     3961 cagagtctgg aagggatata tgagaatctg gatgcggttg ccgaaaagtg gcgcaaaaaa
     4021 ttagaaaccc ataaagagat ggcgtttctg tgccgcgata ttgcccgctt acaaaccgat
     4081 ttgcatatcg acggcaattt acagcaattg cggttggtac ggtaacggcg agccggatac
     4141 gccgcaaatc gtatccggca ttatcacatc agcgcattta ccaggcggta tggtaaagct
     4201 ctacaatatc ctcaagcgtt gcttcacgcg ggttgccacc ggtacaaaca tcatccagtg
     4261 ccgcctgagc cagtgccgaa atgtcttcct tgcgtacacc aacatcacgc aaatgtggcg
     4321 gaataccgac atcacggttg agggcaaaca ccgcttcaac agcggcatta cgcgcccctt
     4381 ccaggctcat accttccact ttcacgccca taacgcgcgc gatatcgcgg tacttctcac
     4441 cggtaaagtc agcgttatag cgcatgacat gcggtaacag gatggcgttc gcaacaccgt
     4501 gtggagtgtt ataaaacgcg cccagtggat gcgccatacc atgcaccaac cctaacccaa
     4561 cattcgagaa gcccataccc gcaacatact gcccgagcgc catttcttct ccggcatcct
     4621 tatcaccagc aaccgatcct cgcagcgccc cagcaatgat ttcaatcgct ttaatgtgca
     4681 gtgcatcggt tagtgcccac gcgccacggg taatataccc ctcaatagca tgagtgagcg
     4741 catcgacacc cgtcgcggct ttcagcgccg gaggcatacc atccatcatg tcagcatcaa
     4801 taaacgccac ctgcgggata tcatgcggat caacgcaaac aaacttgcgc cgtttttctt
     4861 cgtcagtgat cacgtagtta atggtcactt ctgccgcagt acctgctgtg gtggggattg
     4921 ccagaatcgg tacactgggt ttattggtcg gggaaagccc ttccaggcta cgcacatcgg
     4981 caaactccgg gttgttgctg ataatgccaa ttgctttaca agtatcctgt ggagaaccac
     5041 caccaatagc gatcaggtaa tccgcgccgc tattctggaa tacaccgagc ccttctttga
     5101 cgacagtaat tgttgggttg ggcactacgc cgtcgtaaat cgcccatgcc agccctgcag
     5161 catccatctt atcggtcact ttcgccacca cgccgcattg caccagcgtt ttatcggtga
     5221 cgatcagcgc cttctgataa ccacggcgtt tcacctcatc ggttaaagcc ccaacagcac
     5281 cccgaccaaa ccatgccgtt tcgttcagaa tcattctgtt agccatcatc cttctccttg
     5341 ttgctttacg aaattactct tcaatccgta acccataggt tttgaatttc tccagcacta
     5401 cggcaatctc ttcatcgctc agcactggca ccgggtccgt aatcgccagg gtcgtcaggt
     5461 aaagttgcgc cagcacttca acttcatgcg ccagccataa cgctttttcc agattcacct
     5521 cacaagcgat aagcccatga tgttgtaaca aagttgcctt acgatttttg agagccagcg
     5581 caacatgttc agaaagttcg cgtgttccaa aggtcgcata aggcgcgcaa ggaatagaat
     5641 taccgccagc cgccgcaatc atgtagtgaa tagcggggat cgatcggtta agaatggaaa
     5701 ctgccgtgca atgaacggca tgattgtgaa caaccgcgtt ggcatccggt ctgctttgat
     5761 aggctgccat atggaaacgc cattcgcttg aggggagctt tccttcctca tgtttaccgt
     5821 tgccatcaat aaagacaata tgcgactccg tcagtttttc atatggaatg cctgtaggcg
     5881 taatcagcat cccatcctga taacgtacac tgacgttccc cgctgtcccc tggttcagtc
     5941 ccaggcgggt catttccagg caagtgtcaa taatctgacg agcaagttta tttcgttcca
     6001 ttagctacct ctctctgatt caaaacaggg caataatgtt gttcctttca cactattgaa
     6061 ttagccgttt aattacccac catcttcttc ctgattaaca agaaagaaat tcacaagctt
     6121 atattttgtg acctagctca actaatcaca gtaaataact gcaagttctc tttttataac
     6181 cccgttaaaa atgaccgctc ttaaaaatat ttatcaaaac ggtcattttt ccatttcccc
     6241 tcgccagaaa tgcccgtttt cggcacaaac aattaatacg gtcatctggt ttgtgttttt
     6301 tatgatttat tttctgaaac gggcatgaaa tttcgattat taaagtgatg gtagtcacat
     6361 aaagtcacct tccagctaat aagtgtgacc gccgtcatat tacagagcgt tttttatttg
     6421 aaaatgaatc catgagttca tttcagacag gcaaatattc accgatatga agcccgaact
     6481 cgctggtttt gcacttttga aaacataacc gattacgtgc ttaagcttct gaacctaaga
     6541 ggatgctatg ggaaacacat caatacaaac gcagagttac cgtgcggtag ataaagatgc
     6601 agggcaaagc agaagttaca ttattccatt cgcgctgctg tgctcactgt tttttctttg
     6661 ggcggtagcc aataacctta acgacatttt attacctcaa ttccagcagg cttttacgct
     6721 gacaaatttc caggctggcc tgatccaatc ggccttttac tttggttatt tcattatccc
     6781 aatccctgct gggatattga tgaaaaaact cagttataaa gcagggatta ttaccggatt
     6841 atttttatat gccttgggcg ctgcattatt ctggcccgct gcagaaataa tgaactacac
     6901 cttattttta gttggcctat ttattattgc agccggatta ggttgtctgg aaactgccgc
     6961 aaaccctttt gttacggtat tagggccgga aagcagtagt cacttccgct taaatcttgc
     7021 gcaaacattt aactcgtttg gcgcaattat cgcggttgtc tttgggcaaa gtcttatttt
     7081 gtctaacgtg ccacatcaat cgcaagacgt tctcgataaa atgtctccag agcaattgag
     7141 tgcgtataaa cacagcttgg tattatcggt acagacacct tatatgatca tcgtggctat
     7201 cgtgttactg gtcgccctgc tgatcatgct gacgaaattc ccggcattgc agagtgataa
     7261 tcacagtgac gccaaacaag gatcgttctc cgcatcgctt tctcgtctgg cgcgtattcg
     7321 tcactggcgc tgggcggtat tagcgcaatt ctgctatgtc ggcgcacaaa cggcctgctg
     7381 gagctatttg attcgctacg ctgtagaaga aattccaggt atgactgcag gctttgccgc
     7441 taactattta accggaacca tggtgtgctt ctttattggt cgtatcaccg gtacctggct
     7501 catcagtcgc ttcgcaccac acaaagtcct ggccgcctac gcattaatcg ctatggcact
     7561 gtgcctgatc tcagccttcg ctggcggtca tgtgggctta atagccctga ctttatgcag
     7621 cgcctttatg tcgattcagt acccaacaat cttctcgctg ggcattaaga atctcggcca
     7681 ggacaccaaa tacggttcgt ccttcatcgt tatgaccatt attggcggcg gtattgtcac
     7741 tccggtcatg ggttttgtca gtgacgcggc gggcaacatc cccactgctg aactgatccc
     7801 cgcactctgc ttcgcggtca tctttatctt tgcccgtttc cgttctcaaa cggcaactaa
     7861 ctgaacatat tttccgaata aagtgaggaa tctgtaatga aaaaaatcag cttaccgaaa
     7921 attggtatcc gcccggttat tgacggtcgt cgcatgggtg ttcgtgagtc gcttgaagaa
     7981 caaacaatga atatggcgaa agctacggcc gcactgctga ccgagaaact gcgccatacc
     8041 tgcggagctg ccgtcgagtg tgtcatttcc gatacctgta tcgcgggtat ggctgaagcc
     8101 gctgcttgcg aagaaaaatt cagcagccag aatgtaggcc tcaccattac ggtaacgcct
     8161 tgctggtgct atggcagtga aaccatcgac atggatccaa cccgcccgaa agccatttgg
     8221 ggctttaacg gcactgaacg ccccggcgct gtttacctgg cagcggctct ggcagctcac
     8281 agccagaaag gcatcccggc attctccatt tacggtcatg acgttcagga tgccgatgac
     8341 acatcgattc ctgccgatgt tgaagaaaaa ctgctgcgct ttgcccgcgc cggtttggcc
     8401 gtcgccagca tgaaaggtaa aagctatctg tcgctgggcg gcgtttcgat gggtatcgcc
     8461 ggttctattg ttgatcacaa cttctttgaa tcctggctgg gaatgaaagt ccaggcggtg
     8521 gatatgaccg aactgcgtcg ccgtatcgat cagaagattt acgacgaagc cgaactggaa
     8581 atggcactgg cctgggctga taaaaacttc cgctatggcg aagatgaaaa taacaaacag
     8641 tatcaacgca atgccgagca aagccgcgca gttctgcgcg aaagtttact gatggcgatg
     8701 tgtatccgcg acatgatgca aggcaacagc aaactggccg atattggtcg cgtggaagaa
     8761 tcacttggct acaacgccat cgctgcgggc ttccaggggc aacgtcactg gaccgatcaa
     8821 tatcccaatg gtgacaccgc cgaagcgatc ctcaacagtt catttgactg gaatggcgtg
     8881 cgcgaaccct ttgtcgtggc gaccgaaaac gacagtctta acggcgtggc aatgctaatg
     8941 ggtcaccagc tcaccggcac cgctcaggta tttgccgatg tgcgtaccta ctggtcacca
     9001 gaagcaattg agcgtgtaac ggggcataaa ctggacggac tggcagaaca cggcatcatc
     9061 catttgatca actcaggttc tgctgcgctg gacggttcct gtaaacaacg cgacagcgaa
     9121 ggtaacccga cgatgaagcc gcactgggaa atctctcagc aagaggctga cgcttgcctc
     9181 gccgctaccg aatggtgccc ggcgatccac gaatacttcc gtggcggcgg ttactcttcc
     9241 cgcttcctta ccgaaggcgg cgtcccgttc accatgactc gtgtcaacat catcaaaggc
     9301 ctgggaccgg tactgcaaat cgcggaaggc tggagcgtgg aattgccgaa ggatgtgcat
     9361 gacatcctca acaaacgcac caactcaacc tggccaacca cctggtttgc accgcgcctc
     9421 actggtaaag ggccgtttac ggatgtgtat tcggtaatgg cgaactgggg tgccaaccac
     9481 ggagttctga ccatcggcca cgttggcgca gactttatca cccttgcctc catgctgcgt
     9541 atcccggtat gtatgcacaa cgttgaagag accaaagtgt atcgtccttc tgcctgggct
     9601 gcgcacggca tggatattga aggccaggat taccgcgctt gccagaacta cggtccgttg
     9661 tacaagcgtt aatacattac accctgatgt gatgcccggt cgctccggct accgggcctg
     9721 aacaagcaag agtggttagc cggataaaca atgttatccg gctatattgc aggagcgatt
     9781 atgaaacaag aagttatcct ggtactcgac tgtggcgcga ccaatgtcag ggccatcgcg
     9841 gttaatcggc agggaaaaat tgttgcccgc gcctcaacgc ctaatgccag cgatatcgcg
     9901 atggaaaaca acacctggca ccagtggtct ttagactgcc attttgcaac gctttgctga
     9961 ttgctgtcgg caaatcaata gtgaactgac tgaatgccac atccgcggta tcgccgtcac
    10021 cacctttggt gtggatggcg ctctggtaga taagcaaggc aatctgctct atccgatcat
    10081 tagctggaaa tgtccgcgaa cagcagcggt tatggacaat attgaacggt taatctccgc
    10141 acagcggttg caggctattt ctggcgtcgg agcctttagt ttcaatacgt tatataagtt
    10201 ggtgtggttg aaagaaaatc atccacaact gctggaacgc gcgcacgcct ggctcttcat
    10261 ttcgtcgctg attaaccacc gtttaaccgg cgaattcact actgatatca cgatagccgg
    10321 aaccagccag atgctggata tccagcaacg cgatttcagt ccgcaaattt tacaagccac
    10381 cggtattcca cgccgactct tccctcgtct ggtggaagcg ggtgaacaga ttggtacgct
    10441 acagaacagc gccgcagcaa tgctcggctt acccgttggc ataccggtga tttccgcagg
    10501 tcacgatacc cagttcgccc tttttggcgc tggtgccgaa caaaatgaac ccgtgctctc
    10561 ctccggtaca tgggaaattt taatggttcg cagcgcctag gttgatactt cgctgttaag
    10621 tcagtacgcc ggttccacct gcgaactaga tagccaggca tggttgtata acccaggtat
    10681 gcaatggctg gcatccggcg tgctggaatg ggtgagaaaa ctgttctgga cggctgaaac
    10741 accctggcaa atgttgattg aagaagctcg tctgatcgca cctggcgcgg atggtgtgaa
    10801 aatgcagtgt gatttattgt cgtgtcagaa cgctggctgg caaggagtga cgcttaatac
    10861 cacgcggggg catttctatc gcgcggcgct ggaagggtta actgcgcaat tacagcgcaa
    10921 tctacagatg ctggaaaaaa tcgggcactt taaagcctat gaattattgt tagtcggtgg
    10981 aggaagtcgc aacacattgt ggaatcagat taaagccaat atgcttgata ttccggtaaa
    11041 agttctcgac gatgccgaaa cgaccgtcgc aggagctgcg ctgttcggtt ggtatggcgt
    11101 aggggaattt aacagcccgg aagaagcccg tgcacagatt cattatcagt accgttattt
    11161 ctacccgcaa actgaacctg aatttataga ggaagtgtga aatgctgaaa acaatttcgc
    11221 cgttaatttc tcccgaacta ttgaaagtgc tggcagagat gggacatgga gatgaaatta
    11281 ttttttccga tgctcacttt cccgcccatt cgatgggacc gcaggtgatc cgcgctgatg
    11341 gcctgttggt gagcgacttg ctccaggcga ttatcccgtt atttgagctg gacagttatg
    11401 ccccgccgct ggtgatgatg gcggcggtag agggtgacac tctcgatcct gaagtagaac
    11461 gacgttaccg taatgcgctt tcactacaag ccccgtgtcc tgacatcatc cgcatcaatc
    11521 gttttgcgtt ttatgaacgg gcgcaaaaag cctttgcgat cgttatcaca ggcgaacgag
    11581 cgaagtacgg gaatattctt ttaaaaaaag gggtaacacc gtaatctcat accggtacgc
    11641 ccgcatgacg cgggcggtta tcgaatgatg gggtgaaaaa tatgaaagcg gcacgccagc
    11701 aagcgatagt cgacctgctg ctgaaccata ccagcctgac cacggaagct ctctctgaac
    11761 agctaaaggt cagtaaagaa accattcgtc gcgatctcaa tgaattacag acgcagggta
    11821 aaattctgcg caatcatgga cgcgctaaat atatccaccg tcaaaatcaa gacagtggcg
    11881 ctccctttca catcaggctg aaaagccatt atgcgcataa agcagatatc gcgcgcgagg
    11941 cgctcgcgtg gattgaagaa gggatggtga tagccttaga cgccagttca acttgctggt
    12001 atctggcacg ccagttgcct gacatcaaca ttcaggtctt caccaatagc catccgattt
    12061 gccatgaact cggtaaacgc gaacgcattc aactgatcag ttccggcggc acacttgagc
    12121 gcaaatatgg ctgttacgtc aatccctcgc tgatttccca actaaaatcg ctggaaatcg
    12181 atctgtttat tttttcttgt gaagggatcg atagcagcgg cgcactgtgg gactccaatg
    12241 cgatcaacgc tgattacaaa tcgatgctat taaaacgtgc cgcgcaatcg ttgttattga
    12301 ttgataaaag taaatttaat cgttcagggg aagcccgcat cgggtatctg gatgaggtaa
    12361 cgcacattat ttctgatgag cgccaggttg caacttcttt ggtaacagcc tgacggaaag
    12421 ggtagcaggc cggagacgac gccccggcct tgcctgttat cgctcgtcgc gacgaccacc
    12481 caccgcagcc cagatgcggc ggacgtgcac cgtcacttct tcgcgatcgt gatacaactg
    12541 ccgcgcctga atctgagcat ttatgccatg ttcatcaagc tgtgcctgaa tatacgccag
    12601 attgtgtgat acttcttcgt agcgtttttt catcggcagt ttgagattga aaatggtttc
    12661 acggcaccag ccattaacca gccactgcgc catcaatgcc gcaactttag ccggtttttc
    12721 aaccatatcg cataccatcc aggagatatt gctgcgtgtc ggacggaatt tgaaaccgtc
    12781 ttcccgcagc cacgtcacct gcccggtatc catcagactt tgcgccatcg ggccgttgtc
    12841 gacggaataa acccacatgt tgcgcttcac cagttggtag gtccagccgc cagggcaagc
    12901 gcctaaatcc accgcccaca tcccgttcgc caggcgttca tcccactcat ccgcaggaat
    12961 aaacacatga aatgcctctt ccagtttgag cgtggaacga ctcggcgcct ctgccggaaa
    13021 tttcaggcgc ggaatgccca tatagaacgg cgaattattg ttgctgtatg agtaaccggt
    13081 atagcagcat cctggtgcaa taaagaatac atgcacaacc ggacgcttcg gcgtttcata
    13141 gttcgccagc accccagcat cgcgcaaggc agcgcgtagc ggaacagtga acttacggca
    13201 gaatttcagc aactctttgc tttcgttggt atcggcaact tcaacacgca gttcaccgcc
    13261 cttctctact acaccctgta acatgccgac aatgggggta atacgatctt ctggcggcaa
    13321 atgctgcagg agttccccca ccacaaacca ctggcgggca aaaattaatg aactgaacgg
    13381 cagctcacgg attaacttat cgccatcatc aggttgataa cattcataaa tgacataacc
    13441 cgcattctct ttcacgcggg caaaaccgaa aatttcccgc tggccggctt tatcggtaat
    13501 ttctgcggcg cactcttttt caaagcccgg acggcacagc aatacaacct tattcatgac
    13561 ttacgccctt gcgctttaaa cggatagcac caactaacat taacgcccag cccgcaagga
    13621 agctcacgcc gccaaccggt gtgacaaacg cccacaaacg cagatgggac agcgccaggc
    13681 aataaaggct gccgctgaac agcaccgtgc ctaacgcgag gaaaacgcta ctccagtaaa
    13741 accagatact gatgcgacgc tgcattgcca ccgccagacc taagatcgcc agcgtatgaa
    13801 acgcctggta ttcgagaccg gtttggatcc agcccatctc aacggccccc atggttttac
    13861 ttaacacatg cgcaccaaaa gcgcccagtg ccacaaaaat gaagccgcta atggcggcga
    13921 aaatcagcat aaaacggctg gtcatggtcg taccctacgt aaattattgt tcataacgaa
    13981 agcggaattt ttcttgttca gcagcggctt tcgccaggat ccattggcga aaggcggcta
    14041 ttttacccag ttctgcctgg ctgtcatgac aaaccagata aaaagcattt ttactgacca
    14101 gaacatcatt aaacgggcaa acaagacgtc cggcctcgat ttcagattgc gccatcacgt
    14161 tatttgccag cgccactccc tgcccgtgaa tagccgcttg cagcaccatg gcgctatgac
    14221 taaaaattgg cccttgctga acgttgatat gatttaaccc caactgtcgg gtatatgtct
    14281 gccagtcacg gcgtgacgca tcatgtaata acgtatgttt agccagatct tccgggatct
    14341 tcaagggttt ttcgcctgtc agcagtagcg gcgaacacac cggcaataaa tattcggcgt
    14401 acagtttttc cacccgtagc cccggccagt tgccccgacc ataaaatatc gccacatcaa
    14461 catcatccgc cagcttatct tcctgacgat caaccgcctg gattcgaacg tcaattcccg
    14521 gataagctga attaaagctg gaaagtcgcg gaaccaacca atgaatggcg aaactgggga
    14581 gtaaactgac cgtcaacgcc cccttggcgc tacgggcctg gagtttacgc gtcgcttcgg
    14641 ttaattgcga aaatatctct ttgatatcga ggaaatagct ttgcccttcc tcggtcagca
    14701 ggagtgaacg attacggcgg cggaacagtt ttagccccaa aaaatcctca agagacttga
    14761 tttgatgact tactgcggcc tgggtcacaa aaagctcttc tgctgcgcga gtaaaactta
    14821 aatggcgtgc tgcggcatca aaaactcgta aggcatttag cggtggtaat cgtttagaca
    14881 tggcttttaa actttgatgt taaatgaatt taacaattag atcacactgt gtaacctatt
    14941 agttttttta atctgagcca ttataaattg tccgttgagc ttctaccagc aaatacctat
    15001 agtggcggca cttcctgagc cggaacgaaa agttttatcg gaatgcgtat tctggtgaac
    15061 ttttggctta cggttgtgat gttgtgttgt tgtgtttgca attggtctgc gattcagacc
    15121 atggtagcaa agctaccttt tttcacttcc tgtacattta ccctgtctgt ccatagtgat
    15181 taatgtagca ccgcctaatt gcggtgcttt tttttacctt gcgatcgcga attactgttc
    15241 cagttcgacc atctctttca cgtcagtacg gttgatctgc tgtttgttgc cattagcgtc
    15301 tttatacgaa atcataccgg tatcgttatc agtctgtggt ttgccgtcag agacgatggt
    15361 acgtccgtca ttggtgtgca tcacatagtt cgaaccggaa caggcgctca gggcaaaagt
    15421 cagcatacag gcagaaataa ttgcggcagt ctttttcatc atcttctcct taaagcgatt
    15481 aataaatctt aatagtattg attaacaggc taaaattaac gcctaacact attcagcata
    15541 tgttacttac gcggcttcgc caggatatcc agataattct gatggttagc actctccttg
    15601 tatcaaagtg aattttgcgt cacgatcggt gcatcaagcc gaggagtacc atgaacgttt
    15661 ttaatcccgc gcagtttcgc gcccagtttc ccgcactaca ggatgcgggc gtctatctcg
    15721 acagcgccgc gaccgcgctt aaacctgaag ccgtggttga agccactcga cagttttaca
    15781 gcctgagcgc cggaaacgtc catcgcagcc agtttgccga agcccaacgc ctgaccgcgc
    15841 gttatgaagc tgcacgagag aaagtggcgc aattactgaa tgcaccggat gataaaacta
    15901 tcgtctggac gcgcggcacc actgaatcca tcaacatggt ggcacaatgc tatgcgcgtc
    15961 cgcgtctgca accgggcgat gagattattg tcagcgtggc agaacaccac gctaacctcg
    16021 tcccctggct gatggtcgcc caacaaacta gagccaaagt ggtgaaattg ccgcttaatg
    16081 cgcagcgact gccggatgtc gatttgttgc cagaactgat tactccccgt agtcggattc
    16141 tggcgttggg tcagatgtcg aacgtgactg gcggttgccc ggatctggcg cgagcgatta
    16201 cctttgctca ttcagccggg atggtggtga tggttgatgg tgctcagggg gcggtgcatt
    16261 tccccgcgga tgttcagcaa ctggatatcg atttctatgc tttctctggt cacaaactgt
    16321 atggcccgac gggtatcggc gtgctgtatg gcaaatcaga actgctggaa gcgatgtcgc
    16381 cctggctggg tggcggcaaa atgattcacg aagtgagttt tgacggtttc accactcaat
    16441 ctgcgccgtg gaaactggaa gcaggaacgc caaatgtcgc tggcgtcata ggattaagcg
    16501 cggcgctgga atggctggca gattacgata tcaaccaggc cgaaagctgg agccgtagct
    16561 tagcaacgct tgcagaagaa gcgctggcga aacgtccagg ctttcgttca ttccgctgcc
    16621 aggattccag cctgctggcc tttgattttg ctggcgttca tcacagcgat atggtgacgc
    16681 tactggcgga gtacggtatt gccttgcggg ccgggcaaca ttgcgctcag ccgctactgg
    16741 cagaattagg cgtaaccggc acactgcgcg cctcatttgc gccatataat acaaagagtg
    16801 atgtggatgc gctggtgaat gccgttgacc gcgcgctgga attattggtg gattaatgac
    16861 aaacccgcaa ttcgccggac atccgttcgg cacaaccgta accgcagaaa cgttacgcaa
    16921 taccttcgca ccgttgtcgc aatgggaaga taaatatcgc cagttgatca tgctggggaa
    16981 acagcttccg gctttgccag acgagttaaa agcgcaggct aaagagattg ccggatgcga
    17041 aaaccgcgtc tggctgggat ataccgtggc tgaaaacggc aaaatgcact tctttggcga
    17101 cagcgaaggg cgcattgtgc gcggcctgtt ggcggtgttg ttgactgccg ttgaggggaa
    17161 aaccgccgcc gaattacagg cacagtcgcc gctggcattg tttgatgagc tggaattacg
    17221 tgcgcagctt agcgcctcac gcagccaggg gttaaatgcg ttaagcgagg cgattatcgc
    17281 tgcgacgaag caggtttaag cacagaaccg ggtcggataa gacgttcgcg tctcatccga
    17341 cctgattgtt taaccctgac gcgccgcttt cgccatcatc ttcttcagcg catgagaaac
    17401 cgcaacaaac ccaaaggtgg cggtcaccat cgttgccgcg ccaaatccag atgcacaatc
    17461 catccgcttc ggcccttctg ccgtggcttt catcgcacac accgtaccgt ctgactgcgg
    17521 gtacaccagc gcttcagtag aaaacacgca atccacgccg agcttacctt tactgttttt
    17581 cactacgcca aaatcgcttt tcaggcgctc gcgcaacttc gccgccagcg gatcctgaat
    17641 cgttttcgcc agatcggtaa cctgaatctg cgtcgggtca atctgcccac ccgcgccacc
    17701 ggtcgtgacc agcgggattt tattgcgccg acaataagca atcagcgccg ctttgggccg
    17761 tacactatca atggcatcaa tcacgtacga ataaccggca ctcatatatt gcgctacgtt
    17821 atccggcgtc acgaaatcat ccaccaccgt tacacggcac tccgggttaa tctggcgaat
    17881 gcgctccgcc atgacttccg cttttgccag cccgacgtta tcgcgcagag cgtgaatttg
    17941 ccgattggta ttggtgacgc acacatcatc catatcgata agcgtgattg cgccaatccc
    18001 cgtgcgcgcc agcgcttccg ccgcccagga accgacacca ccgataccaa ccacacaaat
    18061 atgcgcgtca gcaaacagtt gcaacgcttt ttcaccatac agacgcgctg tgccaccaaa
    18121 acgctgacgc catgcatcac taattaccac agacatagca cctcagaatt aaaaagggcg
    18181 aggttatccc tcaccctaaa cgcataaatg cctgatgcgc tacgcttatc aggcctacat
    18241 aactcctgca atattttgga tttgcatgat ttgtaggccg gataaggcgt tcacgccgca
    18301 tccggtatga acaacgcgca cttagtcaat aatcaggatg tgaggttacc cctcaccctg
    18361 tcatatccgt aaaaacggca tacagaatat cacatcagcc gctaaagacg ttacctgcgc
    18421 ccggagccgt tttcagcacc caaacccgtc catagtggtt gtaccaacct gcgcggtgtc
    18481 cggcttccgg cccgatccct tgatagatat cgaagtgttg gcctttgatt gcgcccccga
    18541 catccaacgc caccatcaga cgcaattcgt actgaccgtt aaatttgccg ttattatcca
    18601 gcagcggcac ttctgccagc aaggtagtac ctggcggaat aattgaacga tcggaggcaa
    18661 cagaagcacg gccaaccagc ggcaccgcac ttgccccttt caccggtgca aaagattgcg
    18721 gtttaaagaa gacgaaagac gggttctgtt ccagcagctc gcgaacctcg gcttcactgt
    18781 gtgttttgcc ccaatgacga atcgcctgca tcgacatatc ttcttttttc acttcgccac
    18841 ggtcgatcag caccttacca atgctgcgat aggcatgacc gtttttccct gcatagctga
    18901 aaaagttaag cggactgcca tcaccaaagt cgatataccc actaccctgc acatccataa
    18961 tgaagttatc catcagagag ttgctgtaag cgaggatgta tttatcgccc aatgccccgg
    19021 cgtagatctc cgcacgagac ggcagacgac cacgttttgg cggcatacgg taaataggat
    19081 actggaactc gccctggcgg gtatggcgcg cctgaattac cggcgtgtaa tagcctgtaa
    19141 actgcacgtt accatagttg tcggcacctt ccatctgcca ggcatcaatg ccgaactggc
    19201 gcatattgcg ggtatcaccg cctgcgcgca gccactcttg caccgcgtta taaacattac
    19261 tctggttgcc atacagacgc ggtgacgaat tacggatatg gttaatttgc tcggcaaaat
    19321 caccggcgtt aatcggcgcg ccaacggcat ctggctggtt caccagagag aaaggctggg
    19381 taaatttccc gtctttatat tgctgtccgc gatcggttgg tttggaagag caggcggcaa
    19441 gcattgccac aaccgtgccc ataagaaggt actttaccca acgtcctttc attgttctct
    19501 tcttcaggtt aaaaaataag gcgcaacgaa gataacaaac cgccgcgttt aatgaaatga
    19561 gcagcctctc cctgacgcaa attttgcaca aaaaataagc ttagtggttt gtttttgttc
    19621 aaaatcatgc taaatccgtg atcggggtaa aaaaaaggtt gcatgaaaac gcgagcggag
    19681 tatagtgcgc atccacggac gcggggtgga gcagcctggt agctcgtcgg gctcataacc
    19741 cgaaggtcgt cggttcaaat ccggcccccg caaccaatca aatttgatga agtaaaagca
    19801 gtacggtgac gcggggtgga gcagcctggt agctcgtcgg gctcataacc cgaaggtcgt
    19861 cggttcaaat ccggcccccg caaccaatta ttgaacaccc taacgggtgt ttttttgttt
    19921 ctgatctccc ataaaaaaag cgccattcag cgccttttta tcatcccctt ctcgccagcg
    19981 ttgccccatc ggcaaaatac gctttaatcc ccgcaagaat agactccgca acttcctgct
    20041 ggaaagtcgc cgttttcagt ttacgctctt cctcaacgtt actgataaac gccgtttcga
    20101 ccagaatgga gggaatatct ggtgccttta gtacggcaaa cccggcctgt tcaacttgat
    20161 ttttatgcag cttgttgatt tttccgagct tattcagtac cgctttacca aacttaaggc
    20221 tgtcagcaat agtcagcgac tgcaccatat cgaacatggt gtggtcgaca tagcggtcac
    20281 cgcttttgct cacgccacca atcaagtccg aggcgttctg ggtttgtgcc agatattttg
    20341 ccgcagtact ggttgcgcct ttggttgaga gcgcaaacac cgaggaaccg ctcggctgac
    20401 gactggtaaa ggcgtcggca tggatagaga caaacaggtc agcacgctgt ttctgggctt
    20461 ttgctacgcg cacttgcaac ggaatgaaga tgtcttcatt gcgcgtcatg tacaccttca
    20521 tattgccctc tttctcgatc agagagcgca aacggcgagc tatttgcaat accacatctt
    20581 tttcgcgcgt tttgtatttc cccaccgcac cggaatcttc gccaccgtgg ccagggtcaa
    20641 gcataatgac aatcggacga tcgcgccctg ctttacccgg ttgtggacca ctttgcgctg
    20701 gcggcacctg cttttcgagg tcgcctttgt tgtaatcctc cagcagcgcc agcagcgggt
    20761 cctgcatatc ctgtgcattg gccggataga ggtccatcac cagacgctct ttaaacccgg
    20821 cgaccggcgc aagggcaaac agctgcggtt ttacgttttg ctttaattca aaaaccatgc
    20881 gtacggtttg cgggtcaaat tgcccgacgc gcgccgactt gatgaacggg tcgtctgcac
    20941 gaatttgtgc cgccatcccc ttgagtaccg agttcaggtt tacatcttcg atatccacca
    21001 ccacgcgttc agggttactc aacgcgaact gcttatattt cagctgacga tttgattcta
    21061 ccgtcacgcg ggtgtagctg gacgcaggcc agacgcgcac cgccacgacc tggctaaccg
    21121 cagccaggct gacctgactt acgctcaata gccacatagc gcccgcgcct tgcagtaaac
    21181 gacgacggct gattgcagtg ttggatcctg acatgcctct cccgagcaaa agaaatctaa
    21241 gctgtgtaac aagtaaacga ctaatttgac cggaaacttt agcgaaagac gcataatctg
    21301 tcatctaata aacggtaaac attcttttta tattcacggc attactgata aaaaagtcgc
    21361 tctcgcataa aatttacact tgcaccctgc gaaaaaacag aataaaaata caataatttc
    21421 aaataatcat gcaaagaggt gtgccgtggt aaaggaacgt aaaaccgagc tggtcgaggg
    21481 attccgccat tcggttccct atatcaatac ccaccgggga aaaacgtttg tcatcatgct
    21541 cggcggtgaa gccattgagc atgagaattt ctccagtatc gttaatgata tcgggttgtt
    21601 gcacagcctc ggcatccgtc tggtggtggt ctatggcgca cgtccgcaga tcgacgcaaa
    21661 tctggctgcg catcaccacg aaccgctgta tcacaagaat atacgtgtga ccgacgccaa
    21721 aacactagaa ctggtgaagc aggctgcggg aacattgcaa ctggatatta ctgctcgcct
    21781 gtcaatgagt ctcaataaca cgccgctgca gggcgcgcat atcaacgtcg ttagtggcaa
    21841 ttttattatt gcccagccgc tgggcgtcga tgacggcgtg gattactgcc atagcgggcg
    21901 tatccggcgg attgatgaag acgcgatcca tcgtcaactg gaaagcggtg caatagtgct
    21961 aatggggccg gtcgctgttt cagtcactgg cgagagcttt aatctgacct cggaagagat
    22021 tgccactcaa ctagccatca aactgaaagc tgaaaagatg attggttttt gctcttccca
    22081 gggcgttact aatgacgacg gtgatattgt ctccgaactt ttccctaacg aagcgcaagc
    22141 acgggtagaa gcccaggaag agaaaggcga ttacaactcc ggtacggtgc gctttttgcg
    22201 tggcgcagtg aaagcctgcc gcagcggcgt gcgccgctgt catttaatca gttatcagga
    22261 agatggcgcg ctgttgcaag agttgttctc acgcgacggt atcggtacgc agattgtgat
    22321 ggaaagcgcc gagcagattc gtcgcgcaac aatcaacgat attggcggta ttctggagtt
    22381 gattcgcccg ctggagcagc aaggtattct ggtgcgccgt tctcgcgagc agctggagat
    22441 ggaaatcgac aaattcacca ttattcagcg cgataacacg actattgcct gcgccgcgct
    22501 ctatccgttc ccggaagaga agattgggga aatggcctgt gtggcagttc acccggatta
    22561 ccgcagttca tcacggggcg aggttctgct ggaacgcatt gccgctcagg cgaagcagag
    22621 cggcttaagc aaattgtttg tgctgaccac gcgcagtatt cactggttcc aggaacgtgg
    22681 atttaccccg gtggatattg atttactgcc cgagagcaaa aagcagttgt acaactacca
    22741 gcgtaaatcc aaagtgttga tggcggattt agggtaagcg atgaaaatca ccggatgcgg
    22801 caggcgtaac actcgtgcgt cgcatccggc aattacgttt attcccgcga actaaacaac
    22861 gccgccagac cactgcgccg ctcagtacga gtggcgattg ccgcacttaa tatacgctca
    22921 tcggcataca gcgacagacg acgacgcgcg cgggtcaccg cggtgtatac cagctctcgc
    22981 gttactaccg gcgtgcgttg gctcggtaaa atcaacgccg catggtcgaa ctccgatccc
    23041 tgcgatttat gtaccgtcat cgcccacgta gtttcgtgct ctggcaagcg actcggttgc
    23101 acagacttaa tattgccgtc cggcatcgca aaccagacgc gcgtcccctg cccgcgatcc
    23161 agcgcaatac cgatatcgcc attaaacaac ccaagcgcgc tgtcattacg ggcaatcatc
    23221 accggtcggc cttcgtacca acgagagtgc ggatgacgat gaattttgcg cttctgttgc
    23281 ataaactgct caattcgctc attcagtcca gccacgccaa acggcccttc ccgcagggcg
    23341 cacaaaagct ggtactcatt gaacgcctga atgattaaat ccggctcggc acgcgcttgc
    23401 agcagatcca gataacgtcc gtaacccgca agagcttcct caagcatcgc aatataatct
    23461 tcgccgctct gtaaaagtcg tttttcgata tcagtaaaat cctgctgaaa aacggttttc
    23521 actgccgttt tatcaccacg gttgatcgcc gcagctaact gaccaatgcc agaatcgctg
    23581 ccgaaacgat agcttttttg cagcaggcag agactgtcgc gcaaagatgc cgcttctgtg
    23641 ccggttcctg ccggaacgtg agtccccgtc aggcggctta gctgcctggc acgctcggcg
    23701 gtaaagcccg cgttggcata agcgcagata tcgcccagca cagccccagc ctcaaccgag
    23761 gccagttgat cacgatcgcc gagaaagatc actcgcgcat gatctggcaa ggcgtcgatc
    23821 agtctcgaca tcataggcag atcgatcatt gacgcttcat ctaccaccag cacatcaaga
    23881 tgcagcgggt taccggcatg atgacgtaaa cgctggctac ccggctgcgc gcccagcaat
    23941 cggtgcaaag tgctggcatc ttccggaatg cgtttctttt gttcatcggt cagcggtaac
    24001 tgtcgcaaag ccttgccgag agattcggtt aagcgcgcgg cagctttacc cgttggtgca
    24061 gccagacgga tacggcagcg ttcgccgtcg gccatttgaa ttaacgctgc cagcaacttc
    24121 gccacggtgg tcgttttacc ggtgcctggg ccgccggaaa tcaccgagat ccgccgcgtc
    24181 agcgccactg ccgctgcaac tttttgccag ttaatttcat cgcttactgg aaaaagtttg
    24241 tccagggttt gcgccagtag agcttcatca acctcaatgg tatgattcac ctcattgaag
    24301 aagcgcgcta ccgtccgctc gttacaccac atgcgattca aataaagacg atcgccacag
    24361 aggagcatcg gcgtgggttc atctcccctg ctgaccgctt gagaagccag caagcattct
    24421 tcccaatttt gtagctcacc gatttcactg acacaggtcg ccaacagcgg atgcgacgcc
    24481 tcgttatttt ccagtcgtga aagcggcaaa cagacgtgtc cctctccggc atcatgactt
    24541 aacagtgccg ccgcgagggt gacagcagga tgttcatctc ccgccacggt cagggcaaat
    24601 tgcacatcca gcgggcgtag ctgtttgtgc tccacagctt ccagtaattg cttttgcaat
    24661 ttcattacgc ctcctccagg gtcataccgg caaacatctc atccatcagg gcaatcaacc
    24721 cggcgttggg tcgggttgtg taaatccctt gttgcggatg ttctttatca acgccacgca
    24781 ggaacagata gataacgccg ccaaagtgaa gatcatagtc gtaatcagca atgcgatggc
    24841 gcagataacg atgcagtgcc agggtataaa gctgatattg cagatcatag cggtgtgcct
    24901 gcatcgctgc tgccatagcc tgttgggtgt aagccgaact gtcttcaccc agccagttgg
    24961 atttatagtc gagcaggtaa taacgccctt cgtggcggaa taccaggtcg ataaagcctt
    25021 ttaacatgcc gcgtacctgc atgaactcca gcggcgggca gccagcggat agcgggtcaa
    25081 actggcgaat tagtgcatca agctggctgg cgatgagcgg ttcgctaatc ggcaggtaaa
    25141 actccatctc cacctgttta tcgcggtcgg aaagctgact caggctaaca cccgtttcat
    25201 tgagaggtgc ctggaggaca gccgtgatcc actcggtcaa caccggttcc cactgcgatt
    25261 caaagccgcc gagttccagt ttttcctgta cccagttcgg gtcaaccggc tgggtaaaat
    25321 cgaggtcttc aaacaaactg tgcaagaatg tccccggtga cgcaccgcgc gggaactgat
    25381 gcggtgttaa cgtcggttct tcaacgacgc tgaccacgcc tgcggcatca acatccagcc
    25441 gtggcatcag atcctgagcg ataccgtgac cacgctgctg caagccggag tagctggtga
    25501 cacgccagtt atcgccgggc aatcgttgta gtgtcctcgc attcagttct gcagttaaag
    25561 catcattaac ctgccagggc tggttatcac cagtttgtgc cgtttgccag gcaatatcat
    25621 catcgcataa cgcttcaata caggtgcgaa gccctgccgc atcttgcggt tccccttttt
    25681 gcagcaaacg cccgagcgca ctttggtgga cgtcggtgtc acctttttta tcgccacgac
    25741 ggcgcaccag cggtgcaacg ccgagactgc aatgccaaac cgaacgcgtc agcgccacgt
    25801 aaagcaaacg cagatcttcc gccagacgtt cgacctccgc gaggtcgacg ctttctggcg
    25861 cagcattaag atccaaaact gcctcaaacg agtggcgatc gtgataaaac gcctgatcct
    25921 ggacgcggaa attggtaata aatggcaacc agaccagcgg atattccagc cctttcgatt
    25981 tatggatcgt gacaatctgc accagatgtt tatcactttc gaggcgcatt tgttggctgg
    26041 aggcattact gtctggctcg aggatatgtt gcgataacca gcgtaccagc gcatgttcac
    26101 tttccagctg cgttccggct tcttgaagca gttcgctgat atgcaagata tcggtaagac
    26161 gccgctcacc gcctgccgtt gccagcaagt tttctgcaat gttacgcgcc gacatcagcg
    26221 cccgcagcat cggcataacg ccacgtttgc gccagatttg ccgataacca tcgaactctt
    26281 cgactaccgc atcccacgca tgttcgtcat tattcagcgt ttcaatatcc agcgcattca
    26341 gccccatcat tgacgttgcc agcgcactgc gcagggtgtt ctcacgttcg ggcgtcatca
    26401 ccgcctgcaa caaccaaagc atttcctgcg cttccagagt ttcaaaaaca ctgtcgcggt
    26461 tcgaaaggta aacggaaggg atttccagca acgttaaggc atcgcgcacc tgggcggcct
    26521 cctggcggct gcgcaccagc acgctgatgt ccgaagcacg caccggacgc gcgtcgtcgt
    26581 cgttcatcag caacgcttcg ccccgctgtc cggcttgtag ccagtcgcgg atttgcgcag
    26641 cacatacctg cgccatggta ctttgatagt cgccaacgcc gcagctttcg ccttccatca
    26701 gccacatttt catcgcaggc tgcgtttcac ctttaaatac aaaacgtaac gcctgatttt
    26761 tcccggctga tttcactgga ataaacggta tttcgcgaaa catgaacgcg tcatcagtct
    26821 ggctgaaaag cttgttcaca ctgttcacca ttccaggtgc ggagcgccag ttggtgtcta
    26881 aagtgtagtg ggcgtgaact tcactacgcg ccttcatata agtgaagata tccgcacccc
    26941 ggaatgcata tatggcctgc ttcgggtcgc caatcagcaa caatgcggtt tccggctgat
    27001 ggtgccagat acggcgaaaa attcggtact gctgggggtc ggtatcctga aattcatcga
    27061 tcattgccac cgggaatcgc gtacggatcg ccgctgccaa cacctcgccg ctttcgctac
    27121 gcagcgcgga atcgagccga cttaacatgt catcaaaacc caattcgcca cggcggcgtt
    27181 tttcacgcgc tactgtttcg cggatctcag ccaatgctcg ggtgatcacc agatcgcgga
    27241 tcgacaatgg ttctgcaagc agttgatcga tcacctcaaa cagtggatgt cgcggggttt
    27301 cccccccggc cttcgtgcga tcttctaaga aacgctggga gaatttttcc agcgactccg
    27361 gcaactgata actgtttgtc tcttcttctg cccaagcgct gatcttttcg atccatttag
    27421 cctgattgct acggttaaac ttgcgtcgat caataccaga agattcgatc agcgcatcca
    27481 gttcacccac tgcgtcgcgc cactgctgtt ttaccgtatc aatacgcgcc acaatttgcg
    27541 cgtggcggga agccagcgtt tcatcatcgg gcggcggtgc tttgataacc ggcgcttcgc
    27601 cttgcagata acgattaata tcgcgcagca acgcctgcgg ccctttccag gtttcaaaga
    27661 cgacctgggc tatttcacgc ggcagcgggt agcagtggcg acgccagaaa tcggcgcagg
    27721 cctggtagcg tagcagagac tcatcttcaa tcagctgctg ctcaaacagc atgccggatt
    27781 caaaggcatt caggttgagc atgcgctggc aaaagccgtg aatagtaaag actgccgctt
    27841 catccatctg ccgttcggct aacaacaacc actgcgcggc ttgcgcttta tcgtcgatct
    27901 cttccagcag gcgttcgtac agtggattgt cggtggtttc acgcagacag gcgatgcgca
    27961 actcgtggat attgctacgg atacgaccgc gcaattctgc cgtggcagcc tcggtaaagg
    28021 tgaccaccag cagttcttca acgatcagcg ggcggggaaa ggcggcggaa ccgcctagtc
    28081 caagtaacag gcgcaaatag agcgccgcaa tcgtaaaggt tttgcctgtg ccggcagagg
    28141 cttcaatcag gcgctcgccc tgtaagggca agcgcaaagg atctagtgtc tcggcgacat
    28201 cactcattct tttcactcat caggggcatt gtttgctgca acgcgctgac gttctcccac
    28261 actttccagc cttcaggatg cacatattcg gctttcccgt tctggctgcc ggaaatctgc
    28321 gacagaatag ccatgccttg cggctcgacc accgcctgat ggaagaaatc agcaagtttt
    28381 tgcggcgtca gcagttttat ctgggccacg attttatcac gcgaatcgaa gcgcatattg
    28441 ccgcgatcga aatctttact taacttcgat gcttcttcgc cgagcgtttg cggtgcctgc
    28501 agcatctggg taattaccgc ctgctggatt tgcgcaaact catctggctt catcgctcgc
    28561 aattttgcct ctgcggttgg gaaaaacgcc ttgtaacgct cccacaagaa tgaaggctgt
    28621 ttatcattgc tttgcaaaag gaagcccatg ccccactgac gccccacgct cattggaaac
    28681 gcaaacacgg catagcccaa ttgttcttcg gtacgcaact gattgtagaa ccacggctgt
    28741 acgatctgcc ccaacagaga gctataggct gagctggtgt attcatcgta gccagtcggt
    28801 acaaataccg ctgccagtgc ggagtcggtg ctgttaccgg ctttttcaaa gatgacggat
    28861 tgttttttat cgaccactac atctttgttt cgacaccact ctgaaccatc agcgcccaac
    28921 tgtttttgca catcgcgtgc cagcgttgtt gcctgggcct cggtcatgtt gccgataacc
    28981 ataaactctg gtcgagcccc tgattttaag gcgtcgcgat aggccagcac ctctttcaac
    29041 gtaatggagg gcaaaatttt acgccgttca tctcgcgaga agtacggcac ttgcgagagc
    29101 atctgcgcgg gcataatcgc ctgctcaaac gctttaccct tttctgcgga atccatcatc
    29161 tggttatacc aggacttcgc ctgctcaagc tgctcttccg tagcggtata gctaaagtac
    29221 ccctcgagca atgcctggaa cagctgcggc agacgctggg tgtaaccatt agcattaacc
    29281 ataaggccgt tgttagcgtt ggtggaaaaa cttatgccac caaccgacgc ctggttgctt
    29341 aactgatcaa gcgccagccc tgcgagataa tcattgagcg caaacatcac ctgattgcgg
    29401 gcgctgtcca tggctttcgg attacgcaaa atcaggctga catcagcttt gggctcgctg
    29461 gcaaaataac ggcttggcgc atacaccacg cgcagattcg actcatcaac aatcagctct
    29521 ggatggtcgt atttcttctc tgacttaatc agcgagaaat catcaggaat ataagggtta
    29581 agctctggca aagagagcgc aatgtcggcg gcttttttct gccagtcggc gaaagtttgt
    29641 gcgctgattt tatcgacctg atacggcgca tcgacaaagt aagccgtttt gttgtgcggc
    29701 tctttcgggc tgatatacca gatacgcgca ttctgcggcg tcatcatcgc cagacgttcc
    29761 tttactgctt tagcatcgta ccgatcggca atattgactg catccagcgt atgctcaaca
    29821 ggaacgcgaa tcatggtatc tgccagccat tcgacgtaat ccatatcacg ggtgatcgac
    29881 ggataacgga agtcgatatc cagcacattc gccagttcat cgaagtattg tttatcaatg
    29941 cctttttcac gtaacagatt gagatagcta aaaattgccg ccacaacctg atcgcgatta
    30001 gccaggcctt tatcagttaa agacgcagag atcgctaata cgccgctgtt gccgttgacg
    30061 ataggatcgg agttggcgct aatgccctca actaatccct gcttttgcag ccagtcagaa
    30121 agtgtacctg ggctgcgatt gccaatcaga taggtaatca attcatcggt tttactacgg
    30181 aacttcgctg agttgttatc gatgcgaaac tcaacgcgca acactttacg cggcagcgca
    30241 gggacgtaat gaatgataat gcccttttgc gcgtcggtga ctaccggcac ggtgatttcc
    30301 gtttttttgc tctctttgtt cggcacgcga ccaaaggtgt ccgccgccat ttttgccaac
    30361 tccggcagcg gtttattact gtaaataacc gccttcatca aattggcgga atagtacttc
    30421 tcgtggaaat ctttcagcgc ctgctgcacc ggattaccag gtttgtcgct taaagtttcg
    30481 aggttaccac cagaaaactt tgaaccgggg tgtgccgggt taatggtttc tgcactgacc
    30541 tgtgccatgc gcatcccgtc acgcgtacgc gccatggtta attcagcgtt cactgcatta
    30601 cgctcacgtt cggcgtattt cttgtcgagc aagggttctg caatagcatc ggccaggcgg
    30661 tctaccgcac cgggcaaggc gtcgttctca acttccagat agaaagccgt gcgatacggc
    30721 gcagtgctgg cattgtgact accgccgtgc attttgagat attcggccag actgtcagcc
    30781 tgcgggtact ttttcgaccc catcagactc atatgttcaa ggtaatgtgc cagcccctgg
    30841 tacgcctcgg gatcttccag cgacccaacg ggcactacca gcgccgagag cgatttaact
    30901 gcctgcggat cagaaaccag caagaccacc ataccgttat ccagacgtat agcctgatac
    30961 tggcggttat ctttatcact tttacggatg gtttcctgaa tcggctgcca tcccgtttct
    31021 gcctgactta atggtgccca gagggcaaca aacaacaata atgctttgaa ccaggtgctg
    31081 cggggcattc acggacctca taatcaactt tattttctgg acagattcaa caggttaacg
    31141 caaatcatct gccagaattt aatcttgtgc tgcacgagtc agcctatgtt tatataacca
    31201 tcagtccgtg actggtgcgc atcataaagt aagcggatag attgcgcaat ttttatacaa
    31261 cactcatgac tgattaaagc gaaacagcgg taacaggaaa cgttgcgact gttcaacgat
    31321 ggcctccatt gtctctggtg ttaattgccg ccagagccgt tgataccaga tatcatcacc
    31381 ttcgccacgc accatcatgt tgccttcgta agcctgaagg aatttcgtac gggctttttg
    31441 caacgtggaa tcgtcatcca gcatggcatc gttttgcgcg tcataacagg tttttagcca
    31501 cgcgccgcca ctttcaggta acaccagcaa tggcgcggac attccttcac gatacccctc
    31561 aatcagttgt gagaggtaat gcaaagcctg ttcggctgca agcggcggaa aacgccactc
    31621 accgtctttg cgtagaaaaa ggcgactttc gccattacca ccgctggcac agtagacaag
    31681 gtgttccagc caaagttgca ttccctgcgc cacacttaat aaagagggac gccagcgcaa
    31741 caggccatcc ggctgcacct gcggcaacca gccagttatc tgcacaccgt tgcaggcgag
    31801 atcaatttcc atactctgcc ccggctggcg acaggcaatg actctgtcgg caagctgctg
    31861 catctcctgg cactgtgttt cccagaaaat ttcaccaaaa gcgccatacg gtaaatcccc
    31921 tgccgcccgg aagcggcgga acaagcgttc agcatcatcc tgctcaacca gtgcattcaa
    31981 taactgctga ttgatttgat aacggctaag tccttccaga ataaatggct cggtgtctgg
    32041 gatttcgctg tcttcagtac ggaagttcac ctgcaaacgc atctggaaga acgcccgcac
    32101 cggatgtgcc cagaatcgtt gtagcgtttc cagcggcacg gtttccggta aggtaaacgg
    32161 cagcggctga acaaattcag aatgtgcttt accagcctgg ctggccgcag gtagccattc
    32221 acgagcatag ctttgtcgtt cgcctggctg gtagttttgc ggatcaaacg gcatccgggt
    32281 atggaggcaa gtaagatgcg cttttaccct tgcctcgctt tcatcacagt tgagcgcttc
    32341 atcgcccggt agataatgac tttgcccgat gtagtcgatc agttcctgca ccagtaccga
    32401 cgggaaacgt tcactgttat cctgaatgga acgcccgata tagctgatat agagtttttg
    32461 ctgcgcggaa attaacgctt ccaggaacag gtagcggtcg tcatcgcgac ggctacggtc
    32521 gccacgcttc ggtttctggc tcatcaggtc aaagcccaat ggcgcaagct gacgtggata
    32581 aacgccgtcg ttcattccca gcaggcaaac cactttgaac ggaattgaac gcattggcat
    32641 cagagtacaa atattaaccg gtccggcgag aaaacgctgg ctgatacgtt cttgatccag
    32701 acgctgtgcc agttcatcac gcaatagtga cagcggcacc gcgtcgccat actgcgcacc
    32761 taaaccttcg gcgataatcg cctgccattg ttgttcgatc agcgtcatcg ccgcttcggt
    32821 ttccgcatcc ggcaggaaga aggcgttgag catatcgcga caaaccggca accactcttc
    32881 tagcggacgc tcctgtgcca gcccgcgacg ccagatgttt agctgcatta gcagtgaagc
    32941 cagatgcccc accagttctg caattaagcc gctcgattca tcataaggta gaaccgattg
    33001 ccactcgccc tgcgcgctct ccatcgcgta gcccagcaac atacgcgtca ggccaaatcg
    33061 ccaggtgtgt tgtccggtgg cggggagttc cagctcgcga acgttgtcgt catctatccc
    33121 ccaacgaatg ccggattcgt tgacccactg gcgtaaataa cgcagccctt cttcggtgat
    33181 gtcaaaccgc gccgccagca ccggcacatc cagcagcgcc agcacatcct ctgacacaaa
    33241 gcgactgtca ggcagtgata acaggctgat aaacgcttcc agtaccggat gtgactgccg
    33301 cgcacgacgg tcggaaatgg cgtaaggcag gtaacgatcc gcaggtgcgc taccaaacac
    33361 tgcctgaata aacggactgt agctgtcgat atcagccacc atcacgatga tgtcgcgcgg
    33421 agtaagtgtc ggggcttcct ccagcatcgc cagcaagcga tcgtgtaaaa cttcaacttc
    33481 acgctgcggg ctatggcaaa cgtggaatgt gatactgcta tccagtggat caagcgggcg
    33541 tttgttatcg ctacgggaaa actcttcgat gttcacaccc gcaacggcgc ggttttccag
    33601 ttccagaatg tcagactgaa tgttatgcag caggttatct ggcgtcacat cgacaaaagc
    33661 gtccagctcc tggctgctct ccaggtcaga aaggagataa atgtagtcgc gcccaagctt
    33721 cccccatgag gccagcagcg ggttgccgac atcctgttca ccatcgctgt taaagagctg
    33781 tccggcattt tcgctatcgc gaaatagcgg caattcgcga tcttcaaaac tgtgtcgacg
    33841 ctgacgagtc agcagtttcg ccagataagc aggatcttta atatcgcccc agtaataacg
    33901 gcaggggttg gtaaacagga gatggatttc aatatgttta cccagcgcct ggagcgcctg
    33961 gagataaaca ggcggtaacg cggaaatacc gcatataaag acgcgcgaag gtaaccccgg
    34021 cgggcaggtc gtcgcggact ccagcgtttc gataaagcgc tgatagagat tcgcgcggtg
    34081 ccagagcggt tgtccgagtt catgggtata ttccaccagc gccttccaca acggggcttg
    34141 ccaggcctgt gcttctccca gcccttcaac caaatgtcct gtttcccact gtgccagcca
    34201 gtccggacga tagaccagat actggtcaaa caggtccgcc gcttttgagg aaagctggaa
    34261 cagttttcgc ttgtcgctat cgtcagtcag ataatgccgc aacagggtaa agtcttcgcg
    34321 ctccagcaat tgcggcagca gagtcatcag tttccagctc atgctctgtt tgttaaaggc
    34381 gctctctttg gggatttccg gtaacacccg gacgaacata tcccagataa agctcgctgg
    34441 cagcggaaaa tcaatgtttg ccgcaatacc aaacttttgc gacagggtca tttgcagcca
    34501 ctgcgccata ccggtacttt gcaccagaat catctctggt tcgaaaggat cgtccagccg
    34561 ttcgcgttcg acaataaact ccatcaacgc ttccagcacg tccagacgat tggaatggta
    34621 gacccttaac atagcggctc ctgactactg acgattcggg caatgcaggc gggtcatctc
    34681 gccttctctg cccccgggtg aaactagcgt aacgctgatg ctgacacatc ccgcctgcga
    34741 tgtctgcatt cggttgacct gccagttggc aggtggcgaa atcgcgcgca gttgcgtttg
    34801 ctgccaacca tgccgccaga gctgttggta ctggtttctg ctggcgagac tgttcattaa
    34861 tgttcgctgg atacccgata acgcagtgac aatcatcacc atcagcacca tcgcaaccat
    34921 tacctccggc aggctaaagc cttgttgatt cttcagggaa gctgacataa tgccccctct
    34981 ttcagcggac aaaaatcgct ccagccgcgt ggcgaaaaaa caatttttcc atcaatgact
    35041 tcgcctgttc gccacaacga aacgccttca taaccggcaa tcaataaggc ttcattatct
    35101 gccagtaaac gcaaacaaac ctgggcatcg gttccagcgt actgcgagca ctgaactgct
    35161 ggctgcgtct gccaggagtg catttttccc cacgccagcg ccgactgaac gatggcctgg
    35221 cggcgcaatg actgactttc catgctcacg cgagaggcaa aactgcgatc ctgctgactc
    35281 attccttgca atagcaagct acccaaaacc agcagcatca ggaccagagc cagtgacgaa
    35341 acaccttttt cgcggttcac aggttgaatc ctgtcacgct atagctggca tccaccacgg
    35401 tttgcggttc agacttactg gcagcacgca tattaaccgt caacaccggc gagaagccgc
    35461 tgacatcctg acgtacgacc tgaaaagtgt cgataatgat ggcatccgga ttagtgactt
    35521 tatcccagcc cttaccttca caggatgtcg caccgcgtag cgtttccagc acatgctcct
    35581 tcagacgaaa tccaatctgg tcggactctt ttaccggttc gcgatcccag ataccgttac
    35641 tgttcgcatc ccactgcaca atgatacagt caccctgtcc gacaatttcc agcccttcgc
    35701 cggtacagct gccatgacaa taacccgccc tctggagatg cttcgcgacg gtaaataccc
    35761 gcagccagat ttcatcttcc agtgccagct tacgagtgct cgttaaactt tcacgctgta
    35821 acgcaggcag aaagcgtgca gcccccagca acaatacgct actgatcgcc atagcaatca
    35881 acacttccag cagagaaaaa ccttgctctt ttacaggcat ccttctgttt ctccttgctg
    35941 ataaagccgg agtcttcccc acggcgaaac caccagccac cactcgcccg ttgagttttt
    36001 gaagcgaata tgcccggccc atgcggtatt gcgcaggcca aagaaagcaa gcgaaggtgt
    36061 caggtcgctc atttcgactt cgggccagcg tggcacaaag accaatggtg aactgccatg
    36121 acaggtattg gccccagcag cggaactcac aaggcaccat aacgtcccct ccctgataac
    36181 gctgatactg tggtcgcggt tatgccagtt ggcatcttca cgtaaataga gcaaatagtc
    36241 ccgcgcctag ctggcggttt gccaaagccg ttgcgactgc tgccagtatt gccagccata
    36301 gagtccactt gcgcttagca tgaccaaaat cagcatcgcg accagcgttt caatcagcgt
    36361 ataaccacgt tgtgttttca tgccggcagt atggagcgag gagaaaaaaa gacgagggcc
    36421 agtttctatt tcttcggcgc atcttccgga ctatttacgc cgttgcagga cgttgcaaaa
    36481 tttcgggaag gcgtctcgaa gaatttaacg gagggtaaaa aaaccgacgc acactggcgt
    36541 cggctctggc agtatgtttc gtaattagat agccaccggc gctttaatgc ccggatgtgg
    36601 atcgtagcct tcaatctcaa agtcttcgaa acggtagtcg aagatggatt cgggtttacg
    36661 tttgataatc aacttcggca gcggacgcgg ttcgcggctt aattgcagat gagtttgatc
    36721 catatggttg ctgtacaggt gcgtatcgcc accggtccag acaaaatcac ccacttccag
    36781 atcgcactgc tgcgccatca tatgcaccag taacgcgtag ctggcaatgt tgaacggcag
    36841 gccgaggaag acgtcacagg agcgctgata aagctggcaa gagagtttgc cgtctgccac
    36901 atagaactgg aagaatgcat ggcacggtgc cagcgccatt ttatccagtt cgcctacgtt
    36961 ccacgctgaa acaataatgc ggcgcgaatc cgggtcgttt ttcagctggt tcagtaccgt
    37021 agtaatctgg tcaatatgac gaccatctgg cgttggccag gcgcgccact gtttaccata
    37081 cactggcccg aggtcgccat tttcatcggc ccattcgtcc cagatggtga cattgttttc
    37141 gtgtagataa gcaatgttag tatcgccctg caggaaccac agcagttcgt ggatgatgga
    37201 acgcaggtgg caacgtttgg ttgtcaccag cgggaatcca tcctgcaggt taaaacgcat
    37261 ctgatgacca aaaatggaaa gcgttccggt tccggtacgg tcgtttttct gtgtgccttc
    37321 gtcgagcact ttttgcatca gttctaaata ctgtttcatg gttcctcagg aaacgtgttg
    37381 ctgtgggctg cgacgatatg cccagaccat catgatcaca cccgcgacaa tcatcgggat
    37441 ggaaagaatt tgccccatgc tgatgtactg cacccaggca ccggtaaact gcgcgtcggg
    37501 ctggcggaaa aactcaacaa tgatgcgaaa cgcgccgtag ccaatcagga acaagcctga
    37561 gacagctccc attgggcgcg gtttacgaat atacaggttg aggataataa acagcaccac
    37621 accttccagc agcagttcgt aaagctgtga tgggtggcgc ggcagcacac cgtaagtgtc
    37681 gaaaatggat tgccactgcg ggttggtttg cagcagcaaa atatcttctg tacgggagcc
    37741 tgggaacagc atggcaaacg ggaagttcgg gtcaacgcgg ccccacaatt caccgttaat
    37801 aaagttgccc agacgcccgg caccaagacc aaacggaatg agtggtgcga taaaatcaga
    37861 gacctggaag aaggaacgtt tagtacggcg ggcgaagata atcatcacca cgataacgcc
    37921 aatcaggccg ccgtggaaag acatgccgcc gtcccagaca cggaacagat acagcggatc
    37981 ggccataaac tgcgggaaat tgtagaacag aacataacca atacgtcccc cgaggaagac
    38041 gccgaggaag cccgcataga gtaagttttc aacttcattt ttggtccagc cactgcccgg
    38101 acgattcgcc cgacgtgttg ccagccacat tgcaaaaatg aaacccacca gatacatcag
    38161 gccgtaccag tgaagcgcca cgggtcctat tgagaaaatg accggatcaa actccggaaa
    38221 atgcagatag ctactggtca tctgtcacca caagttcttg ttatttcgct gaaagagaac
    38281 agcgattgaa atgcgcgccg caggtttcag gcgctccaaa ggtgcgaata atagcacaag
    38341 gggacctggc tggttgccgg ataccgttaa aagatatgta tatgatccgc gctataaccc
    38401 tccgcgaatc agcccgccca tgccgcgacg ctccataaag gctgcaacct gatgacgaac
    38461 ttcggtcgcc agttgcgctt ccagactacg ctgcgcaaga ttttctgctt cggcaaaatc
    38521 aatgcgccgc agcaggtatt ttacccgcgc tacagaacgt ccgttcatag acagatggcg
    38581 atacccaagc ccaatgagga ttgccacgca catgggatcg cccgccattt caccgcacaa
    38641 acggagatcg ataccatgta tttccgcttc ccgggcgatc atcgccagag ctcgtaacat
    38701 tgcaggatga agactgtcgt aaatgttcgc cacccgggta ttgttgcgat caacggccag
    38761 aatgtattga gtcagatcgt tggtgccaac agagatgaaa tcgacccgct ttgccagatg
    38821 cggcagcata aataccattg acggcacttc cagcatgatg ccgatacgtg gtttgggaat
    38881 ttcgtaaccg atcatctcct cgacttcacg tccggcacgt tcaatcaggc ggcgtgcttc
    38941 gtcaacttca tcgaggctgg tgaccatcgg caacagaata ttcaggttgc ccgtagcggc
    39001 attagcacgc agcatcgccc gcacctggat caagaagatc tccggctgat cgagcgtaat
    39061 gcgaatacca cgccaaccca ggcatggatt ctcttcgctg atcggcatgt aaggcagctg
    39121 cttatctgct ccgacatcca gcgtacgcaa ggtgacgggt ttatcattga acatttgcag
    39181 catcccctga tactgcgcca cctgttcttc ttccgacggg aaaccacttt gcagcatgaa
    39241 tgggatttca gtgcgataaa gaccgatgcc atcaatacgg ctgcccagtt tttcttcatg
    39301 ttccgggctt aaaccagcat tgagcatgac ttttatacgc tcaccgcttt ttaactgcgc
    39361 gggtaaattg acgtcatctt ccgccagacg gctaagctca atctcttcac taattagccg
    39421 ctgatattct tgcagcagta ccggctccgg atcgaccagc aattcaccgc gatagccatc
    39481 aacgatcagc gtcctacgat gcagcaccga aggctgaata tccgcgccca tcacggtagg
    39541 gatccccagc gcacgtacca tgatcgcagc atgggagttg gctgcgccat ctcgcacgac
    39601 aacaccgact aagcgatcct ggggcagctc agcaagagtt gtcgctgaca gttcatctgc
    39661 caccagaatg aaacgttccg gccaggcgtt cggcccttga ttagcgtcat caagatgaaa
    39721 cagcaatcgc tgacccagcg cacgtaaatc gccagcccgc tctttgagat agttatcgct
    39781 tagcgcggca aactgttcgg caaatttttc aatgaccgtt tttaccgccc actctgccac
    39841 cgagccttta tcaacctcgg caaacaattc gcgacgcagc cgggtgtccg aaagcaggtg
    39901 cgagtaaaga tcgaaaatag ccgccgtttc tttttgtgct ccggcggcaa agcgtttgct
    39961 gtagcggcga aactcgtttg ccgcttcttc cagcgccccg gtcagtcgtt cgcgttccag
    40021 agccggatcc agcgttgatg cctgatacac ctgttccatt aaaggtaacg tggcatcctg
    40081 ccagccttcg gcaatcgcca caccaggtgc tgccggtaat gcgcggattc gcgtctggcg
    40141 atattgccca aacagggcag tcaactgcga ctgagaaaga atagctgcca tctgggtggc
    40201 aagcgtcacc aggaaggatt cttcactttc gtcatactgg cgcaactctc gttgctgtac
    40261 caccagtaca ccaagcaact ggcgacgttg aataattggt acgcctaaaa acgcgcggaa
    40321 acgttcttct tttatggagg ggatgtattt gaagctgggg tgcttttgcg catctgcaag
    40381 gtttatcggt tccgccagcc tgccaaccag gccgacgatc ccttcatcaa acgcgagcgt
    40441 tacagtgcga ccgcgtggtt ttttcagccc ccgggtcgcc atcaggtagt aacaacgtcg
    40501 atcatgatcg gccaggtaga ccgaacagac ctcggtatcc atcgcaagac agatgtcggt
    40561 aaccagaata tttaacgcct cattcaggcg tggtgcgctg gctacctttt cgactatttc
    40621 gcgcaggcga gtgagcataa ttggcgtgac ttaacctctt ttacgtcgat aagcagatgc
    40681 gttttgtggt tttggcgtat tttcctgcag tgacatcacc acactcgcga actctttcat
    40741 taccctacgg tagacatcac gtttaaatga caccacctgt ctgaccggat accagtaact
    40801 tacccatcgc cagccgtcaa actctggtgt actgctggtt tgcatattga tttctgcatc
    40861 gccgcttacc agctgcaaga gaaaccattt ttgtttttgg ccgatacaaa ccggcttcgt
    40921 gtcccaacgc accaaacgtt tcggtaattt gtagcgcaac cagttacgcg ttgaagcaag
    40981 gattcgaacg tctttgcggc ttaatcctac ttcttcaaac aattcacggt acatcgcctg
    41041 ctctgcggat tctccggggt tgattccgcc ttgcggaaat tgccaggagt gctgaccaaa
    41101 tcgccgggcc cacattacct gcccctggcg attacaaatc acgataccta cgtttgggcg
    41161 gtagccatcg tcatcaatca ccggactacc tcaaaataaa gctttatata cgaatgattg
    41221 tttcatactc caggaagacg gtaaaccact ctctgcaggg cattacacac taataacaat
    41281 tgaataactc acagttatgt gcagagttat aaacagagga aggggtggat agccgttttt
    41341 attcaccttt tctgtggata gagttgtgaa gaactacgga attactacgg gaaaacccgg
    41401 agcattctga ataagcccga tatagacatt ttaaaatatt cattattagt caatgcatta
    41461 catgtaattt gagtaatgaa aaagagtaat tcgtgaccca ggtcacacct ctcatttacg
    41521 ggttggcgaa agatcaacca atgccgtatt tatccacaga atgtgccact aagttaagca
    41581 ctgaaccact aaaaactgga gtttcgtcgc acgtcaaggc tgtaaatgaa aacagtagtg
    41641 gaggtttttc acagttatcc cagctttctg tggataacat ggtgtaagat cctgtttatt
    41701 ttcagtgacc agatttggaa aacctattgc agtgttgcgc aactcgatta ccggcagcct
    41761 aaaaagcaat ataaatcaga gaattgaaca acacatgtgg aaaaagttac actgcgaata
    41821 ttcggcacat aattgctgtt tgttttttaa ccaaggcatc atgacatgtc ccaacctcgc
    41881 ccactgctct ctcctcccga aactgaagaa cagttgttag cgcaagcaca gcaactttct
    41941 ggttatacat tgggagaact ggcggcactt gccgggctgg ttacgccaga gaatttaaaa
    42001 cgcgataagg gctggattgg cgtgttactg gagatctggc taggtgccag cgcagggagt
    42061 aaacctgagc aagattttgc tgctctgggc gtggaactta aaactatccc tgtggatagt
    42121 cttggtcgtc cgctggaaac aacattcgtt tgtgttgccc cgttaacggg taatagcggg
    42181 gtgacctggg aaaccagcca tgtgcgccac aagctcaaac gcgtactgtg gataccggtt
    42241 gaaggcgagc gcagcatccc gctggcgcag cgtcgcgtag gatcaccgtt gctgtggagc
    42301 ccgaatgaag aggaagaccg acagctacgc gaagactggg aagaattaat ggatatgatt
    42361 gttctcggtc aggttgagcg gatcaccgct cgtcacgggg aatatttaca gataagaccg
    42421 aaagcagcga atgcgaaagc gcttaccgaa gccattggtg cccggggcga acggattctg
    42481 acgctgccac gcggctttta tttgaagaag aatttcacca gtgcactact ggcccgtcat
    42541 tttctgatcc agtagccatc gctttgacct gccgctttcc gggcatataa ttaccgcttc
    42601 atttttttgg cagggctttt tagatgttat ttgcatggat aaccgatcct aacgcctggc
    42661 ttgcgctcgg tacgctgacg ctgctggaga tcgttcttgg gatcgacaat attattttcc
    42721 tttctctggt ggtggcaaag cttcccacag cacaacgtgc tcatgcgcgc cgtctggggt
    42781 tggcgggagc catggttatg cgtctggcgc tgctggcatc aatctcttgg gttacgcgcc
    42841 tgacaaatcc gctttttaca atattcagtc aggaaatttc tgcccgtgat ttgattctgc
    42901 ttctgggtgg cttgttcctt atctggaaag ccagcaagga aatccacgaa tccattgaag
    42961 gtgaagaaga agggctgaaa acacgcgttt catcattcct cggcgctatc gtgcagatta
    43021 tgctgctgga tattatcttc agcctcgact cggtgattac cgctgtgggt ctgtcagatc
    43081 acctgtttat tatgatggca gccgtggtga ttgccgtagg cgtgatgatg ttcgccgcgc
    43141 gctcgattgg tgattttgtc gaacgccatc cttcggtaaa aatgctggcg ctctctttcc
    43201 tgattctggt gggctttacc ctgattctgg aaagtttcga catccacgta ccgaaaggtt
    43261 acatctactt cgcgatgttc ttctctattg cggttgaaag cctcaacttg attcgcaaca
    43321 aaaagaatcc gctctgatac tccgtacgct ctcctgcggg agggcgtact cactcctttt
    43381 accctcacag tttaagattt tcctgctttc aagatatata acgtcgattt ataaacagac
    43441 tattatcata ggtaggccaa atgaggataa ccggatgaaa aaatgggcag taataatttc
    43501 tgcagtcgga ctggcgtttg ctgtttccgg gtgttccagt gattacgtca tggcgaccaa
    43561 agatggccgt atgattttga ccgatggaaa acctgaaatt gatgatgata ccgggctggt
    43621 gagttatcac gatcagcaag gtaacgcgat gcaaattaac cgtgatgatg tttcgcaaat
    43681 tattgaacgt taacaaataa ggtcagcatc cggctggcct taagattttt ctctttccct
    43741 tttcccttcc ctctgccatt tttatattcc ttatgtcgtg attataaaaa ggaaacggct
    43801 atgcaatatc accgtatacc ccacagttcg ctggaagtca gcacgctggg gcttggcacg
    43861 atgacgtttg gtgaacagaa cagcgaagcc gacgcccacg cacaactcga ctatgccgtc
    43921 gctcagggca ttaaccttat cgacgttgcc gaaatgtacc cagtacctcc gcgccccgaa
    43981 acgcaagggt taaccgaaac ctacgtcggc aactggctgg cgaaacatgg cagccgcgaa
    44041 aagttaatta tcgcctccaa agtgagcgga ccgtcgcgca ataatgacaa gggcatccgc
    44101 ccggatcagg cgctggatcg gaagaatatc cgcgaagcgc tgcatgacag cctcaagcgc
    44161 ctacagactg attacctcga tctttatcag gtgcactggc cgcagcgccc gaccaactgc
    44221 ttcggcaaac tcggttatag ctggacggat tctgcgcctg cggtttcgct gctggatacg
    44281 ctggacgcac tggcagagta ccaacgcgcg ggaaaaattc gttatatcgg cgtgtcgaac
    44341 gaaactgcat ttggcgtaat gcgctacctg catctggcgg acaaacacga tctgccgcgt
    44401 attgtcacca ttcagaaccc ttacagtctg ttaaaccgca gttttgaagt aggtctggca
    44461 gaagtcagcc agtatgaagg ggtcgaactg ctggcctatt cgtgcctggg tttcggcacg
    44521 ctgaccggga aatatctcaa tggtgcaaaa cccgctggcg cacgtaatac gctctttagt
    44581 cggttcaccc gctatagcgg tgagcaaacg caaaaagccg tcgcggcgta tgttgatatc
    44641 gccagacgtc atggccttga tcctgctcag atggcgctcg cgtttgttcg ccgtcaaccg
    44701 tttgttgcca gcactctgct gggcgcaacc acgatggatc agctgaaaac taacatcgaa
    44761 agtttgcatc tggagttaag cgaagacgta ttagctgaaa ttgaagcggt acatcaggtt
    44821 tatacttatc ctgcaccata aagattaacg ccggttaaaa ccggcgttaa atattaatga
    44881 cggcgctgcc agatccacag cgccgttatt gccagcgcaa atagcgcacc gaagccaatg
    44941 ccaatgggca cgaccgggat gcctaccatt accgccagcg agtaaatgcc cagcatcaac
    45001 aacatggcgc tgttttcgcc aaggttttgt actgcaatcg cattccccgc cccgacgctt
    45061 tttttacccc gctcctgtag caacgcattg agcggaacga caaaaaagcc ccccagcacg
    45121 ccaatcagca tcaacaaggc ataggctggc agcagctcgt gttgcaggga gaaaatcagt
    45181 accaccacgc caatcaaaat cccggctggc atacagcgtg acacggtttc cagcgtaacc
    45241 agcttcgctg ccgcacctgc gccaaccacg ataccaatcg ctaccatcgc gttgagatag
    45301 gtcggcgtag cgttatcggt aatgcccagc gccaccggta cccacagcac caacaggaaa
    45361 cgcagcgtga cacccgctcc ccagaataaa ctggtgccca ccagcgaaaa acgcgtttca
    45421 ccattgcacc atagcgaggt gcaggcattc aggaaactgc gggtcatgtt gatgagattc
    45481 caggactgcc ccggacgcgc cgccgccagt ttgggaatgt agatattggc aacgaccgca
    45541 ccaccgtagg ccagtacgca tgcggccagg gcgaccagga catgccagtc agccagcaca
    45601 ccaccggcta cggaaccgag taaaatcgcc gctatggcag aagcttccat taaaccgtta
    45661 gcttttacta acttactacc cgtggttaat tcgccgagaa taccgtattt cgccggtgaa
    45721 taggctgcag caccaacacc caccagcgta tagccgagaa acggattgat accaaagcag
    45781 atactggctg cgcccagcag cttcaggccg ttggcaaaca tcatcacccg gcctttggcg
    45841 aagctatccg ccacctgccc gacaaacggc gccaaaagaa tgtaagcacc tacaaacacc
    45901 atttgcagga tgggctggct ccactccgga cagaactgcg ctttcagtaa cgccagagtg
    45961 gcaaacagta gcgcgttatc gccaaacgca gagagaaact gcgccacgat aaccgctttc
    46021 atccccttcg accacaacga agtgttagtg tgcactgact cactcatcgt gttgttccgc
    46081 ttcgtctacc cagcttttca acgtgacaaa gtcaggtttg ccgctgccaa gtaatggcat
    46141 ctgtttcaga tagcgaatat cgcgcggtac agcaagctcc ggcacgccgt gctcgcgggc
    46201 atactgttgc aacttatcgc gcgtcagttc gttatctgtg gtgaaaagca ccagtgcctc
    46261 gcctttgctg gcatcgctct taatcgcagt ggcatggact ttatctggcg aaacaccaag
    46321 tgccagttgt tccaccattt ccagcgacac catttcgcct gcaattttgg caaagcgttt
    46381 tgcgcggccc tgaatctgca caaagccctg ctcgtcaaaa cgcacaatat cgccagtgtc
    46441 ataccagccg cgctccattt cgccgcgaac attctcggcg gtgggcactt ccagtacacc
    46501 tggcttctcc acccgcagat agccgttcat tatgttcggc cctttcagtt gcaggcgtcc
    46561 gccctcttcg ataccaggga ccgacaacag gcgtgcatcc attcctggta gaatacgccc
    46621 taccgtaccg ggtttcgccg ccatcggtac gttgatagaa acgacaggcg cgcattcggt
    46681 cacaccgtag ccttcaagga tgcgcaggcc aaatttatcc tgccaaagct gtttggtact
    46741 ttcttgtagt ttttctgcgc ctgccaccac atagcgtaga cgatagaagt catacgggtt
    46801 ggcgaaacgc gcgtagtgac cgaggaaagt cgaggtaccg aacaacacgg tgcaactgcg
    46861 gtcatacacc aagtccggca caatgcggta atgcagcggg cttggataaa ggaacacttc
    46921 tgcacctgta agcagtggcg taaacaggcc taccgtcagc ccaaaggagt gaaacagcgg
    46981 taatgccgac ataaagcgat cgttggtggt gaagtcggca atcgttttaa tctgctcgac
    47041 gttcgccaga atgcttttat ggctatggac gacgcctttt ggatggcctt cagaaccgga
    47101 ggtaaagagg atcagcgcct cttcttccgg ctgctgtttt acctgcgcca ggcgcggcat
    47161 cagcaaatga gcgaagatcc atactttgtc ggcagtggtg acatctgctt ttaaatcttc
    47221 cagatagacc cagcgcacct gagtaagttg ctccggcaga tgccagagtt tgcctttatc
    47281 gagaaactgg cgggaagtga agatggtttt gatttcagcc gccgtaataa cactggtcag
    47341 cccttttacc ccggcagtgt agttcatcat tgcgggaatg cggcgacgcg cgatggcccc
    47401 aaaaatcact gccgcactga tgcctgcatt gggcagcatt aagccgatgc gttcgccttc
    47461 aacactgtat ttttcaagga tgcgtccaac aaacagcgtt ttcgtaagca atttgcgata
    47521 ggagtctggg gtaaagttga cgtcttcgac acatttcttc ccggctccga agcggtacat
    47581 tgcactcagt aaagattcgt acagagtttc acgcgggcgc accgccatac gcgcttccat
    47641 cattatttga tgcagcattt cgccagcgat tttgcgacgg tcacgggcac gcggcgcatc
    47701 cggcatcgcc acctgcgttg gaggcaaaat atgcagagta atttgcggga acaagcgacg
    47761 tttaaccaga cctttcaggc ggctgaagtg cgtaagttcc gccccttcaa tacgcacagg
    47821 aataaccgtt gcaccagact tcgccgcgac aaaacccgcg ccatcgtaga ttttcatcag
    47881 cgagcctgta gtggtgatgc gtccttcagg gaaaatcacc actggtcggc cctgttccac
    47941 cagacgtacc agatgtttaa tagccatagg ttgcgtcggg tcgagaggaa caaagtcgat
    48001 aaatgatttc agccaacgca tataccactg ttggcttatt gaggtgtaaa cggcaaacac
    48061 tggacgcaca ggtaaaaaca gtccaagcaa aatgccatca ataaaagaga cgtgattagg
    48121 cgtaattaga acgcgctcgc ccttcagtgc ctgggtgtca cccgtaacgc gaacgcgata
    48181 caaaacacgg cacaaatttc gaaaaaagct aaaaagcatt ccaactccct ttgctctgat
    48241 tcagtaaaag cgaatggagg gagattacac gagataaaga acgcgagcga cagtaaatta
    48301 ggtgcgaaaa aaaacctgcg catccgcgca ggttggtgca agagacaggg tacgaagagc
    48361 gtaccgaata atctcaccaa tcaatacctc tgggatcacc actttagcaa cctgaagcca
    48421 aacgccacca gcggtcaatc gcaacagcgt ttcgcaaagt gtaaccaaag gtttgcaatc
    48481 tctttttttg tcttacttat ttgcgtttgg ctcacattcc cacgatgaaa acacgccacc
    48541 ccttgaacca acgggcgttt tccgtaacac tgaaacaatg taagcgttta cccactaagg
    48601 tattttcatg gcgaccataa aggatgtagc ccgactggca ggcgtttcag tcgccaccgt
    48661 ttcccgcgtc attaataatt cacccaaagc cagcgaagct tcccggctgg ctgtgcatag
    48721 tgcaatggag tctcttagct atcacccgaa cgccaacgcc cgtgcgctgg cgcagcagac
    48781 cactgaaacg gtcggtctga tcgttggtga tgtttccgat ccgtttttcg gtgcaatggt
    48841 gaaagcggtc gaacaggtgg cttatcacac cggtaatttc ttattgattg gcaacggtta
    48901 ccacaacgaa caaaaagagc gtcaggctat tgagcaactg atccgccatc gctgtgctgc
    48961 gttggtcgtc catgccaaaa tgatcccgga tgccgattta gcctcattaa tgaaacaaat
    49021 gcccggtatg gtgctgatca accgtatcct gcctggcttt gaaaaccgtt gtattgctct
    49081 tgacgatcgt tacggtgcct ggctggcaac gcgtcattta attcagcaag gtcatacccg
    49141 cattggttat ctgtgctcta accactctat ttctgacgcc gaagatcgtc tgcaagggta
    49201 ttacgatgcc cttgctgaaa gtggtattcc ggccaatgat cggctggtga catttggcga
    49261 accagacgaa aacggcggcg aacaggcaat gaccgagctt ttgggacgag gaagaaattt
    49321 cactgcggta gcctgttata acgattcaat ggcggcgggc gcgatgggcg ttctcaatga
    49381 taatggtatt gatgtaccgg gtgagatttc gttaattggc tttgatgatg tgctggtgtc
    49441 acgctatgtg cgtccgcgcc tgaccaccgt gcgttaccca atcgtaacga tggcgaccca
    49501 ggctgccgaa ctggctttgg cactggcgga taatcgccct ctcccggaaa ttactaatgt
    49561 atttagtccg acgctggtac gtcgtcattc agtgtcaact ccgtcgctgg aggcaagtca
    49621 tcatgcaacc agcgactaac cgcagttaaa gcaattccag cgccagtaat tcttcgatgg
    49681 tctggcgacg gcgaatcaac cgcgcctgac cattatcaaa cagaacttct ggtaacagcg
    49741 gacggctatt gtagttggat gacattgatg cgccatatgc ccctgtatca tgcagtacca
    49801 gataatcacc tgccttcact tccggcaagg cgcgggtttc aacatttccc ccttcctgct
    49861 gggtaaagac atcgcccgat tcacataacg gcccggcgac gacggtttcc accgttggcg
    49921 cgtgttccag agaacgacca tcagctgcca gggcactgat atggtggtaa ctaccgtaca
    49981 ttgccgggcg catcagatcg ttgaacccgg catcaaccag cacaaagtgg cggctaccca
    50041 tttgtttgac gctccgcacc tgagtaatta atacgccaga ctgcgctacc aggaagcgac
    50101 ccggttcaat ttccagtttc acagggtggc ccaaatggcg agcgatttgc tcacgcgcgg
    50161 cattccacag accataataa tgttcggtat caaccgcctc ttcaccctgt tgataaggaa
    50221 tagaaagccc accgcccgca gaaatagcct gtaaatcctg accgaattcg atgacctgac
    50281 gcaccatagc accacacacc tgctccagat gggcataatc aacgccagaa ccaatgtgca
    50341 tgtgaatgcc gactagctgc agatgatgac gttgtatcac gtccagtgcg gcgggcagat
    50401 cggtgtacca gataccgtgc ttgctgtttt cgccaccggt attggttttt tggctatgtc
    50461 cgtgaccaaa ccccggatta acgcgcagcc atacccgatg ccctggcgaa acctggccca
    50521 gttggtcgag catatcaaca gaacccgcat tcaccggaat ttgcaattca ctgacgcgtt
    50581 caagcgtcgc ctgatcgata acatctgccg taaaaacaat atcatcgggg tgcgtttgcg
    50641 gattgtaacc cgccgccaac gcacgctcta tttcgcctaa cgagacggaa tccactttca
    50701 cgccctgctc acgcattaag cgcaaaatat gaatattgga acaggctttc tgtgcaaagc
    50761 gcaccacttc aaactgtttc agcgctgcaa tctgccgacg aataatttgc gcatcgtaga
    50821 cccacaccgg gcagccaaat tcagcgggca aacgcagcag attttcggcg gtgagatcgg
    50881 tatcggtgct gaacagtgaa tgtggcataa caaactccag atgagtgctt ttttatgatt
    50941 acgccacatc ataaaaagaa taaaaaatat cgatttatgt cgaatctatg caaaaatgat
    51001 atggattacc ggattgcgag agagcgctaa tggccgccgt taacttacgt catattgaaa
    51061 tttttcatgc ggtaatgacc gccggaagcc tgactgaggc agcacacctg ctacacacct
    51121 cacagccaac cgtcagccgc gaactggcgc gctttgagaa ggtgatcggg ctgaaattgt
    51181 ttgagcgcgt acgtggacga ttacatccta ccgtgcaagg actgcgtctg tttgaagaag
    51241 tgcaacgatc ctggtacgga ctggatcgca ttgtcagcgc cgcagaaagt ctgcgcgagt
    51301 ttcgccaggg agaactgtct attgcctgcc tgccggtctt ttcgcaatct tttttaccgc
    51361 agctcctgca accctttctg gcacgttatc ccgatgtcag cttaaatatc gtgccccagg
    51421 aatcaccgct acttgaagag tggctctcgg cccagcgtca tgatttagga ctcactgaga
    51481 cactccatac gcctgcggga acagaacgta ccgaattact ctctttagat gaagtgtgtg
    51541 tgttacctcc gggtcatccg ctggcggtaa aaaaggtatt aacgccggat gattttcagg
    51601 gtgagaatta catcagcctt tcccgtactg acagctatcg ccagttgctg gatcagctat
    51661 ttactgaaca tcaggttaaa cgacgcatga tcgtagaaac ccacagcgcc gcgtcagtct
    51721 gcgcaatggt acgggcgggg gtaggtgttt cggtggttaa cccgctcacc gcactagatt
    51781 atgcggcaag cggtttagtg gtgcggcggt tcagtattgc ggttccgttc accgtcagcc
    51841 tgatccgccc cctgcaccgc ccgtcatcag cgctggttca ggcgtttagt gggcatttac
    51901 aagcggggtt accgaaactg gtcacttctc ttgacgctat tttgtcgtca gctacgacag
    51961 cataaaagcg acagcatcct cggcatggat cgccgcggta tcaaacacag gcaaaacact
    52021 gcgctcttct ggcaccagta aaccaatttc tgtgcagcca aaaatgacgc cctgtgcgcc
    52081 ctgttctgca aggcgagcaa tcacttgcgc ataataagcg cgtgacgctt cggtaaattt
    52141 ccccagacac agttcttcaa aaataatctg attaattttc gcccgttcat ccgcttcagg
    52201 aataagacaa ttgatggaaa attgttccgt cagccgcccg cgataaaaat cctgttccat
    52261 ggtgtaacgc gtacccagca gcgccacacg agtcattccg gccccggtaa ttgcacgtcc
    52321 ggtggcatcc gcaatgtgta agaaaggcag agtgcaacgt gactcaatgg catccgccac
    52381 tttatgcatc gtattggtac ataacacaat accttctgcg cccgcccgct gtaagccaag
    52441 cgccgcctca gccagaatgt ccccggtttt atcccattcc ccgcgacgct ggcactcttc
    52501 tatttcatga aaatcgacgc tatgtagcag catttgcgca gagtgaagcc caccaagccg
    52561 ctgtttaatg ccttcattta tcagacgata gtaaggaatg gtggattccc agctcattcc
    52621 tcccagcaaa ccaattgttt tcattcctct ctcctgatgt gtgttaccgc aacaaggctg
    52681 aataaggaac gagacaaacg cctcaacggc caggtgccca atctctatta acgaaaaaag
    52741 ggccggatgt acagcacatc cggcccgtga aatcagacgc cgatatttct caacttctcg
    52801 cctgccatca gtttgcgttc gatatgttcc agcgtgacat ttttggtttc cagaatgagc
    52861 cagaaagtaa tgcccacaaa cgcaatgttc aacgcagtgt agagccagaa cgtaccggca
    52921 gcgccaatgc tatcaagcag tgtcaggaag gccgcgccga taatcatatt cgacacccag
    52981 tttgtcgtcg tcgaacaggt aataccgaaa tcgcggcatt tcagcggctg aatttcagag
    53041 cacaggatcc acaccactgg cgcggcgctc atcgcataac cggcaataca catcatcgtc
    53101 atgccaacag agagccagga caagccactg gaagccgtac cgttatcaaa ctgcatcagg
    53161 caatagccca gcaccagagt gcctaacgcc atcacgctga aaccaatttt cagagccggt
    53221 ttacgacctg ctttatctac cgtaaagacc gcaatgaagg tcgcgaacat aaaggtcagt
    53281 cccacgacca gagtcgcaat catctgttgt tctgtggtcg taaagcccgc cattttgaag
    53341 atacgcggcg cgtagtacat gatgatgttc ataccggtaa actgctgcat cgcctgcaac
    53401 aacataccga gaaacacagc acgacggacg ttacggttga tcttaaacag tgcccaaccg
    53461 ccctgtttta acttcaggct ttcacgaatt tcgttgagtt cttctcgcgc tttttccgac
    53521 gtatcgcgca gcatacgcaa cacttcttcc gcctcaatat gacgcccctt ttccgccagc
    53581 cagcgcgggc tatttggcag gaagaccacc agaataatca gcagaactgc tggtaaagca
    53641 agaaccccaa cattgcgcgc cagttaccgc tataactgaa cgctgtatcg gataaaaacg
    53701 ccagcacgat gccgagtgtg accatcaact ggtacatact gatcatctta ccgcgaacgt
    53761 acttgccatt tcagaaagat acagaggagc ggtgtaagac gcgatcccga cagcaatgcc
    53821 cagcaccaca cgagcggcga ttaacatctc tacgctggtc gcaaaagcgg accctataga
    53881 accgagtaca aacaggatgg cccccgccat caggctgtat ttacgcccca ggcggaacga
    53941 cagccaacca ttaaacagcg caccaattgc tgcgccgagc atcatgctac taaccaccca
    54001 ttcctgcaaa cgactggtca gcacaaagtg atcggtaatg aacggcaacg ctccggcgat
    54061 tacgccgata tcaagaccaa ataacaatcc tgcgaccgca gcagctaccg aaacaaacat
    54121 attcatacgc cgcgtatcac gcaaagaacg tggcgttaaa gcagattccg tattgatagt
    54181 aaccattttt tcctgccagc agagagtaag acattgtgaa aaaatacgtg aacaattcac
    54241 acaggtgtca ggtcggaaac agcataaata tggattaaat tgctgcgaca tgccgttatg
    54301 tgatggatat tccaatttta aaattaagtt gaattattga gattattatt aaccacctaa
    54361 ttttaaagta gataaaattc ataaagttca ttaattgata attgatatgg attatttcat
    54421 aaccatgata tggattatga tgatctagag atataaaaaa ccctgccatg cggcagggtc
    54481 ataaaagtaa gaggaatgaa ttaacgcgcc agccaaccgc catccacagc aatggtataa
    54541 ccatttacat aatctgaagc gctggaggca agaaacacta ccggccccat caggtcactc
    54601 ggcagtcccc aacggccagc tggaatgcga tcgagaattt ccgcgctacg ttgttcatct
    54661 gcccgcagtt gttgggtatt gttggtcgcc atgtaacccg gagctatcgc attaacatta
    54721 atgttgtgtt tagcccattc gtttgccatc aatcgcgtca cacccatcac tccgcttttt
    54781 gatgcggtat aagaaggcac acggatcccg ccctggaagg agagcattga cgcgatattg
    54841 ataatcttgc cgccattgcc ttgcgcgata aagtgtttcg ccgctgcctg agacatgaag
    54901 aatacgctct tgatattcag gttcatgaca tcgtcccagt ctttttcgct gaactcgaga
    54961 gcgtcttcgc ggcgaatcaa tccggcgtta ttcaccagga tatcaatatg accaaattca
    55021 gctaccgcgc gatccagcag tccaggaatg ccatcaatct ttcgcagatc ggcggtcagg
    55081 cttaaaaaac gacgccccag cgccgtgacc tgctcgatgg tttcagtcgg ttcaacgatg
    55141 ttaatgccaa caatgtcaca gcccgcttgc gccagcccca acgccatccc ctggcccagc
    55201 ccagtatcac aaccagtgac gaccgcaact ttaccttgga gagaaaatgc acttaaaatc
    55261 ataacaacac cttattcgtt tatgcccaca actagcgcaa atctttaacg gccacatggt
    55321 ccatatcatt aaagacctgg ttttcaccga ccatgcccca gataaaggta taagctttgg
    55381 ttccgacgcc ggaatggatc gaccagctcg gggagatcac cgcctgctcg ttatgcatca
    55441 caatgtgacg cgtttcttgc ggctgcccca tcatgtggaa aacgcaggcg tcatcatcca
    55501 tattgaaata gaaataaact tccatccggc gctcgtgggt gtgacacggc atggtgttcc
    55561 acaagttacc tggagccagc tccgtcagcc ccatactcaa ttgacaggtt tccagtacat
    55621 ccgggacaaa atatttgtta atcgtgcgac ggttactggt gaggttatcg cctaacgtga
    55681 ctggagatac ttcgtccggt gtgacttttt tggtgggata cgtcgtatgc gcgggtgcgc
    55741 aattgtaata aaacttcgcc ggagtggcgg tatcaatact ggcaaagaca acttcttttg
    55801 cacctttacc aacatacagg gcgtcgcggt gaccgatttc atagcattga ccatcgacag
    55861 taatcgtacc ggcaccgcca atattgataa cacctaactc gcgacgttca aggaaatagc
    55921 ttacgccgag ttgtttacca acttccccgc caacggaaac cgttttagtt atcggcataa
    55981 tgccgccaac aataattcgg tcaatgtggc tgtaaaccat ggtgtactca tcggcgacaa
    56041 ataccttttc aaccaaaaat tcattacgca gcccttgggt atccagcgtt tttgcgtgcg
    56101 caccgtggat gctctgtctt acgtccacat caacctccga taatcagtga agcgtgaaaa
    56161 tagaaaacga tagtgaaaca ctgtttcgtt ttctctataa agggatgata tgcccatggg
    56221 ctagggtttt caattaaaaa taaaacaatg ttttattttt aatttaagga ttaaaaaaaa
    56281 tgcagtgtcg atcacgaata tgtcatgaga atggaaaaat gagggagccc agaaaatgta
    56341 tgtcgacttg ccagccatca ttattcaaca gattaatgac ggcagatatt tatcggtaag
    56401 gaggaaatgc agagctactc gtcacgttca atggtcaatg ccacgcccta gcccccgccg
    56461 atacaaagcg ttgccagccc tttgcgggca ttacgtttca ccatttcatg aaccagagaa
    56521 accaggattc ggcaaccgga agcgcctatc gggtgaccga gtgcgatcgc gccaccattg
    56581 acattgaccc gacgctcatc ccactcaagc atcttgccaa ccgaaagcgc ctgtgcagca
    56641 aacgcttcat tagcctcgat aagatcgaca tcagccaact gccagcctac acgctccagg
    56701 caacggcggg tcgcatacac cggcgcaatt cccatcaatg ccggatctac accaacgctg
    56761 gcaaatgcgc ggatgcgggc cagcacgggt aaattcaacg ctcgtgcttt ggcttcgctc
    56821 atcatcatta ccgcagctgc gccatcgttt atggatgatg cattccccgc ggtcacagaa
    56881 ccgagactat caaatgaagg atttaaacgg gctaaacctt ctgcactggc gtcagtgcgt
    56941 ggctgttcat cggtatcaac aaccaacgtc tgaccgttac tttggatcat taccgggacg
    57001 atctcatctt taaatcgtcc ggcgtcaatc gccgctcgcg ctttttgttg cgagctaagt
    57061 gcgtaagcat cctgcaactg acggctgatg ccatattcgc gagccagatt ttcggcggtg
    57121 acaccaatat gataatcatt gaaggcatcc cacaacccat catgcaccag actgtcaacc
    57181 aactggctat tgccaagctg tgcaccggtg cggctatcag tcagaacatg tggtgcgcgg
    57241 ctcatgtttt cctggccacc ggcgatgaca atatcagcct cgccacactg tatcgcctga
    57301 gtagccagat gcagtgcttt aagaccggaa ccgcaaacgt cattaatcgt gattgcagaa
    57361 acgctgttag gaagaccacc tttaatagcc gattgccttg ccggattctg ccctgcccct
    57421 gcagtcaaca cctgaccaag aattacttca tccaccgcat atgcaggaac gccggtacgt
    57481 tctattaacg ctttcacgac cagactacca agttccacgg cggaatgacc cgctaacgca
    57541 ccacgaaagc agccgatagg tgtccgtaac gccccgacaa tcacaacgtc tttcatcaca
    57601 acctcgcacc aattaacagt gcaatagtaa atgattgtta attacagtta tcttaattgt
    57661 ttaaaaaaag tgatttttat cacaaaggaa atatgcctga gcagtagtca gagtcataac
    57721 tggcacgtaa ggtttgcaac cactaaccca ccaatagatg ggtagatagg gcgttaatct
    57781 cccatactta acttggttta tggtaaattg ccctccattt tgtttaattt gtagatgata
    57841 cgttcagata atgtctaata tttggtctaa agaagaaact ctgtggagtt tcgcgctcta
    57901 cggcacagcc gttggtgcag gcacgctatt ccttcctatt cagttaggtt cggcaggggc
    57961 tgtggtcctg tttattactg ctctggtcgc ctggccttta acatattagc tacataaagc
    58021 cttatgccag ttcatcctct catcaaaaac atcagcaggt gaagggataa cgggcgcggt
    58081 aacacactac tatggcaaga agattggtaa tctgattacc acgctgtact tcatcgcctt
    58141 ttttgtcgtc gtgttgatat atgcagtggc aattaccaac tcacttacgg aacagctggc
    58201 aaagcatatg gttattgatc ttcgcatccg tatgttggtg agtctgggtg ttgtattaat
    58261 tctgaatctc atttttctga tgggacgtca tgccactatt cgggtaatgg gatttttggt
    58321 attcccattg attgcctatt tcttatttct ttccatttac cttgtcggta gttggcaacc
    58381 tgatctatta acaacccagg tagagttcaa tcagaatacc cttcaccaga tatggatatc
    58441 gattcccgtg atggttttcg cctttagcca tacgcccatt atttctacgt ttgccataga
    58501 cagacgtgaa aaatatggcg aacacgctat ggataaatgc aaaaaaatta tgaaagtcgc
    58561 ttatctcatc atctgcataa gtgtactgtt ctttgtcttt agctgcctgc tttctattcc
    58621 atcttcgtat attgaagcgg ctaaagaaga aggggtcacc attttatcgg cgctttctat
    58681 gctgccgaac gccccagcat ggttgtcaat ttccgggatt attgtcgcag tagttgcgat
    58741 gtcgaaatca ttcctgggta cgtactttgg cgttattgaa ggtgccacag aggtcgtcaa
    58801 aacaacacta cagcaggttg gtgtaaagaa aagtcgtgca tttaaccacg cactatcaat
    58861 tatgttggta tcgctgatta ccttcattgt ttgttgcatt aacccgaacg cgatttcgat
    58921 gatttacgcg atcagcggcc cgctcattgc catgatactt ttcatcatgc ctacgctgtc
    58981 aacgtatctc atcccggcgc ttaaaccctg gcgttccatc ggaaatctga ttacgctgat
    59041 cgtgggtatc ctgtgcgtat cggtaatgtt ctttagctaa tatccgttgt ggatggttca
    59101 aaattgctac aattttgaac cattccattt ttcataatat tcatttgtta ttattccgcc
    59161 ctatcacttc tctcgtcata tcctagaatt atcagttagt taaacacaca aagagatctg
    59221 aacatcttaa ataagactat ttaagatgca taacttagat tcgcaagata tcttctggtc
    59281 attcaaaaac aatttgcgat atagatattc tcataccatg agatagttat ctggaccacg
    59341 ttcgaccatg caagattaaa tcagtgaaac ataatcatat ttaataacga gaaatgcatt
    59401 tttaaatatc ttaatcagaa ttatagcaaa tacagattaa tcgca
//
LOCUS       DAETPZ010000016        58109 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.016, whole genome shotgun sequence.
ACCESSION   DAETPZ010000016 DAETPZ010000000
VERSION     DAETPZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.016"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(26..280)
                     /locus_tag="K0W59_001100"
     CDS             complement(26..280)
                     /locus_tag="K0W59_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBI3022229.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            435..1007
                     /gene="yeiP"
                     /locus_tag="K0W59_001101"
     CDS             435..1007
                     /gene="yeiP"
                     /locus_tag="K0W59_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HBI3022230.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1230..2696
                     /locus_tag="K0W59_001102"
     CDS             1230..2696
                     /locus_tag="K0W59_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBI3022231.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2814..3800
                     /gene="yeiR"
                     /locus_tag="K0W59_001103"
     CDS             2814..3800
                     /gene="yeiR"
                     /locus_tag="K0W59_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HBI3022232.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQLL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3839..4552
                     /gene="lpxT"
                     /locus_tag="K0W59_001104"
     CDS             3839..4552
                     /gene="lpxT"
                     /locus_tag="K0W59_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HBI3022233.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4964..5530
                     /gene="mepS"
                     /locus_tag="K0W59_001105"
     CDS             4964..5530
                     /gene="mepS"
                     /locus_tag="K0W59_001105"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HBI3022234.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAMG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5711..7267
                     /locus_tag="K0W59_001106"
     CDS             5711..7267
                     /locus_tag="K0W59_001106"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBI3022235.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVARHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHLVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            7349..9163
                     /locus_tag="K0W59_001107"
     CDS             7349..9163
                     /locus_tag="K0W59_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBI3022236.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWHFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9164..10258
                     /locus_tag="K0W59_001108"
     CDS             9164..10258
                     /locus_tag="K0W59_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HBI3022237.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10258..11283
                     /locus_tag="K0W59_001109"
     CDS             10258..11283
                     /locus_tag="K0W59_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3022238.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11285..12874
                     /gene="yejF"
                     /locus_tag="K0W59_001110"
     CDS             11285..12874
                     /gene="yejF"
                     /locus_tag="K0W59_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HBI3022239.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12878..13222)
                     /locus_tag="K0W59_001111"
     CDS             complement(12878..13222)
                     /locus_tag="K0W59_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022240.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13555..14745)
                     /gene="bcr"
                     /locus_tag="K0W59_001112"
     CDS             complement(13555..14745)
                     /gene="bcr"
                     /locus_tag="K0W59_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HBI3022241.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAVFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14773..15468)
                     /gene="rsuA"
                     /locus_tag="K0W59_001113"
     CDS             complement(14773..15468)
                     /gene="rsuA"
                     /locus_tag="K0W59_001113"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HBI3022242.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15618..17378
                     /locus_tag="K0W59_001114"
     CDS             15618..17378
                     /locus_tag="K0W59_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBI3022243.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKDGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDPPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17503..17787
                     /gene="rplY"
                     /locus_tag="K0W59_001115"
     CDS             17503..17787
                     /gene="rplY"
                     /locus_tag="K0W59_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HBI3022244.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEINVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17926..18933)
                     /gene="yejK"
                     /locus_tag="K0W59_001116"
     CDS             complement(17926..18933)
                     /gene="yejK"
                     /locus_tag="K0W59_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HBI3022245.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19115..19342
                     /locus_tag="K0W59_001117"
     CDS             19115..19342
                     /locus_tag="K0W59_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HBI3022246.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            19362..21122
                     /gene="pbgA"
                     /locus_tag="K0W59_001118"
     CDS             19362..21122
                     /gene="pbgA"
                     /locus_tag="K0W59_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HBI3022247.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDTVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21197..21273
                     /locus_tag="K0W59_001119"
     tRNA            21197..21273
                     /locus_tag="K0W59_001119"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21231..21233,aa:Pro,seq:ggg)
     gene            complement(21376..23967)
                     /gene="yejO"
                     /locus_tag="K0W59_001120"
     CDS             complement(21376..23967)
                     /gene="yejO"
                     /locus_tag="K0W59_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HBI3022248.1"
                     /translation="MHQSCSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVDKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24287..24934
                     /gene="narP"
                     /locus_tag="K0W59_001121"
     CDS             24287..24934
                     /gene="narP"
                     /locus_tag="K0W59_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HBI3022249.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGNKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25145..26197)
                     /gene="ccmH"
                     /locus_tag="K0W59_001122"
     CDS             complement(25145..26197)
                     /gene="ccmH"
                     /locus_tag="K0W59_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HBI3022250.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDTNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26194..26751)
                     /gene="dsbE"
                     /locus_tag="K0W59_001123"
     CDS             complement(26194..26751)
                     /gene="dsbE"
                     /locus_tag="K0W59_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HBI3022251.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26748..28691)
                     /gene="ccmF"
                     /locus_tag="K0W59_001124"
     CDS             complement(26748..28691)
                     /gene="ccmF"
                     /locus_tag="K0W59_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HBI3022252.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28688..29167)
                     /gene="ccmE"
                     /locus_tag="K0W59_001125"
     CDS             complement(28688..29167)
                     /gene="ccmE"
                     /locus_tag="K0W59_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HBI3022253.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29164..29373)
                     /gene="ccmD"
                     /locus_tag="K0W59_001126"
     CDS             complement(29164..29373)
                     /gene="ccmD"
                     /locus_tag="K0W59_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HBI3022254.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29370..30107)
                     /gene="ccmC"
                     /locus_tag="K0W59_001127"
     CDS             complement(29370..30107)
                     /gene="ccmC"
                     /locus_tag="K0W59_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HBI3022255.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30149..30811)
                     /gene="ccmB"
                     /locus_tag="K0W59_001128"
     CDS             complement(30149..30811)
                     /gene="ccmB"
                     /locus_tag="K0W59_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HBI3022256.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30808..31425)
                     /gene="ccmA"
                     /locus_tag="K0W59_001129"
     CDS             complement(30808..31425)
                     /gene="ccmA"
                     /locus_tag="K0W59_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HBI3022257.1"
                     /translation="MLEAREVLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31444..32046)
                     /gene="napC"
                     /locus_tag="K0W59_001130"
     CDS             complement(31444..32046)
                     /gene="napC"
                     /locus_tag="K0W59_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HBI3022258.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32056..32505)
                     /gene="napB"
                     /locus_tag="K0W59_001131"
     CDS             complement(32056..32505)
                     /gene="napB"
                     /locus_tag="K0W59_001131"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HBI3022259.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32502..33365)
                     /gene="napH"
                     /locus_tag="K0W59_001132"
     CDS             complement(32502..33365)
                     /gene="napH"
                     /locus_tag="K0W59_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HBI3022260.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33352..34047)
                     /gene="napG"
                     /locus_tag="K0W59_001133"
     CDS             complement(33352..34047)
                     /gene="napG"
                     /locus_tag="K0W59_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HBI3022261.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34054..36540)
                     /gene="napA"
                     /locus_tag="K0W59_001134"
     CDS             complement(34054..36540)
                     /gene="napA"
                     /locus_tag="K0W59_001134"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HBI3022262.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36537..36800)
                     /gene="napD"
                     /locus_tag="K0W59_001135"
     CDS             complement(36537..36800)
                     /gene="napD"
                     /locus_tag="K0W59_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HBI3022263.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36790..37284)
                     /gene="napF"
                     /locus_tag="K0W59_001136"
     CDS             complement(36790..37284)
                     /gene="napF"
                     /locus_tag="K0W59_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HBI3022264.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37692..38180
                     /gene="eco"
                     /locus_tag="K0W59_001137"
     CDS             37692..38180
                     /gene="eco"
                     /locus_tag="K0W59_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HBI3022265.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38330..39361)
                     /locus_tag="K0W59_001138"
     CDS             complement(38330..39361)
                     /locus_tag="K0W59_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HBI3022266.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRTKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFELVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39595..39954)
                     /locus_tag="K0W59_001139"
                     /pseudo
     CDS             complement(39595..39954)
                     /locus_tag="K0W59_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40172..41815)
                     /gene="yojI"
                     /locus_tag="K0W59_001140"
     CDS             complement(40172..41815)
                     /gene="yojI"
                     /locus_tag="K0W59_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HBI3022267.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPHAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVPLLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41891..42485)
                     /gene="alkB"
                     /locus_tag="K0W59_001141"
                     /pseudo
     CDS             complement(41891..42485)
                     /gene="alkB"
                     /locus_tag="K0W59_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
     gene            complement(42485..43549)
                     /gene="ada"
                     /locus_tag="K0W59_001142"
     CDS             complement(42485..43549)
                     /gene="ada"
                     /locus_tag="K0W59_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HBI3022268.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43623..44678)
                     /gene="apbE"
                     /locus_tag="K0W59_001143"
     CDS             complement(43623..44678)
                     /gene="apbE"
                     /locus_tag="K0W59_001143"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HBI3022269.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44790..45911)
                     /gene="ompC"
                     /locus_tag="K0W59_001144"
     CDS             complement(44790..45911)
                     /gene="ompC"
                     /locus_tag="K0W59_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBI3022270.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46649..49321
                     /gene="rcsD"
                     /locus_tag="K0W59_001145"
     CDS             46649..49321
                     /gene="rcsD"
                     /locus_tag="K0W59_001145"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HBI3022271.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPRIEKYISDIDSYVKSLL"
     gene            49338..49988
                     /gene="rcsB"
                     /locus_tag="K0W59_001146"
     CDS             49338..49988
                     /gene="rcsB"
                     /locus_tag="K0W59_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HBI3022272.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGLLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50188..53037)
                     /gene="rcsC"
                     /locus_tag="K0W59_001147"
     CDS             complement(50188..53037)
                     /gene="rcsC"
                     /locus_tag="K0W59_001147"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HBI3022273.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53312..54088)
                     /locus_tag="K0W59_001148"
     CDS             complement(53312..54088)
                     /locus_tag="K0W59_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HBI3022274.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQISGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54093..55742)
                     /locus_tag="K0W59_001149"
     CDS             complement(54093..55742)
                     /locus_tag="K0W59_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HBI3022275.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLAFLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVSSMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPGSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55743..56465)
                     /locus_tag="K0W59_001150"
     CDS             complement(55743..56465)
                     /locus_tag="K0W59_001150"
                     /inference="COORDINATES: protein motif:HMM:NF036531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022276.1"
                     /translation="MTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGG
                     GEDWRWVGQGVPDILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEE
                     EMSFTLQPVTSNEIDSDALYLDEITLTCEQDAVLRYGQVEVPLPPGADVERTTWGISV
                     NKPNAAKQQGQLLEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYV
                     RSYAPAQQSVAAGSEWTGMQVK"
     gene            complement(56462..58021)
                     /locus_tag="K0W59_001151"
     CDS             complement(56462..58021)
                     /locus_tag="K0W59_001151"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022277.1"
                     /translation="MTTSELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFY
                     PLGRNNVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPS
                     LTTDKQGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVY
                     RVGDKPAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLS
                     AELQQNGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETP
                     QEIFRNNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRL
                     MQLAGPGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQA
                     DNMPLLHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILD
                     TPESPLADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGG
                     DATQTAAILMV"
BASE COUNT        14190 a        15677 c        14291 g        13951 t
ORIGIN      
        1 tttgcttact gcggattaaa gatgtttaag gcgcggtcag catttccaga tcgataagcc
       61 aggtcatggc ctgctgacgc gagttaccgc acatttcagc gctggcttgt aagcctgcac
      121 agactttcgg tcgcagtggc gaggtgaaaa ttttacagcg ttgctgttca tcaagctgaa
      181 tgcagggcgt attggcgggc ttgccatctg gcataccggg aatagggctg gaaattgaag
      241 gggcagtgca acacgccccg caacccggac ggcattccat acgttgttct cttaaggtta
      301 accaccggat tgatgagcgc gcacagtacc accttttttg caccagcaaa agtgcgaata
      361 ccacttgccc gaaaggccca tcgcgagtac tttgtcgcga tatttttgac attttcgact
      421 acaggaattt ttcgatgcca agagcgaacg aaattaaaaa aggtatggta ctgaattaca
      481 acggcaaact gctgctggct aaggatattg atattcagtc gcccactgcc cgcggtgccg
      541 ctacgctgta caaaatgcgt ttttctgatg tccgtaccgg gctgaaagta gaagagcgtt
      601 tcaaaggtga tgatatcgtt gacaccgtga cgctgacccg ccgttacgtt gacttctcct
      661 atgtcgatgg caacgaatat gtctttatgg ataaagaaga ctataccccg tataccttca
      721 ccaaagatca gattgaagaa gagttgctgt ttatgccaga gggcggcatg ccggacatgc
      781 aggtgctgac ctgggacggt caactgctgg cgcttgagct gccgcagacc gttgatctgg
      841 aaatcgttga aaccgcacca ggcatcaaag gggcatctgc cagcgcccgt aacaaaccgg
      901 cgacattgag cactggtctg gtgattcagg taccagaata cttaagcccg ggcgaaaaaa
      961 ttcgtatcca tatcgaagaa cgccgttata tggggcgtgc tgactaactt cagccgcatg
     1021 cagaaaaggg atagctcagg ctgtcccttt tttaatttat tataccaatc ttctattttg
     1081 cgcttcctga acaccttacg ccctcaattt tcactcgttg attgatcgcc ctcacacttc
     1141 atcgcattaa caatccagac caatttcaat tgctgtcata taactttaca ctgtcattgt
     1201 taatttatcg ttactaagac gtgactccta tgaagacaat tgcctccgtt acgctcccgc
     1261 atcatgtaca cgctccacgc tacgatcgcc agcagttgca atcacgtatc gttcattttg
     1321 gctttggcgc ctttcaccgc gctcatcagg cgttactgac cgatcgtgtg ctgaatgccc
     1381 agggcggcga ctgggggatc tgtgaaatca gcttgttcag cggtgatcaa ctgatgagcc
     1441 agctccgcgc acagaaccat ttatataccg tgctggagaa aggtgcggac ggcaatcagg
     1501 tgataattgt cggtgccgtt cacgaatgcc ttaatgcaaa actggattcc ttagcggcaa
     1561 ttattgagaa attttgcgag ccacaggtgg caattgtttc cctgacgatt accgaaaaag
     1621 gctattgtat tgacccggcc accggtgcac tcgacaccag taatccgcgg attattcacg
     1681 atctacaaac ccctgaagaa cctcactccg caccgggtat tctcgtcgaa gcactgaaac
     1741 gccgccgtga gcgcggcctt acaccgttta ccgtgctctc ctgcgacaat attcccgaca
     1801 atggccatgt ggtgaaaaac gcggtgctga gaatggcaga aaaacgttcg ccagaactcg
     1861 ccgggtggat aaaagaacac gtcagttttc cgggaaccat ggtcgaccgc attgttccgg
     1921 ctgcaaccga cgaatcactg gcagaaatca gccagcatct gggggtaaat gatccctgcg
     1981 cgattagctg cgaaccgttt atccagtggg tggttgaaga taacttcatc gctgggcgtc
     2041 ctgcctggga agtcgcaggt gtacaaatgg tgaatgatgt cctgccatgg gaagagatga
     2101 aactgcgtat gcttaatggc agccactctt ttctcgctta tctgggttac ctctcaggat
     2161 tcgcctatat cagtgattgc atgcaggatc gcgtatttcg ctatgctgcc agaacattaa
     2221 tgctggatga gcaagcgccg acactgcaaa ttaaagatgt cgatttaaca caatatgcgg
     2281 ataagttaat tgcacgtttt gctaatccgt cgctgaaaca taagacctgg caaatcgcga
     2341 tggatggcag ccagaaatta ccgcaacgta tgctggcagg tattcgcata catctggggc
     2401 gtgaaacgga ctggtcgttg ctggcattag gcgttgctgg ctggatgcgt tacgtcagcg
     2461 gcgttgatga tgccggaaat gccattgatg ttcgcgatcc gcttagcgat aaaattcgcg
     2521 aacttgttgc gggcagcagc agtgaacaac gcgtaaccgc cctgctttcc ctgcgtgaag
     2581 ttttcggtga tgatctgcca gataacccgc attttgtgca ggccatcgaa caagcctggc
     2641 aacaaatcgt acaattcggc gcacatcagg cgctattaaa caccctcaaa atttaacgat
     2701 ttctgcggtt aaagcggatg aagctcacct tcgtccgctc tccccttctc ttttctgcct
     2761 tttttagcca ggattaacgc tcagttaact taccagagta ttgcggagcc gttgtgacca
     2821 ggaccaacct catcaccggt tttctcggca gcgggaaaac cacgtcgatt cttcatctgt
     2881 tagcccataa agatcccaac gaaaaatggg cggtactggt taatgaattt ggggaagtcg
     2941 gaattgatgg tgctttgctc gccgatagcg gcgcattgct gaaagagatc cccggcggct
     3001 gcatgtgctg cgttaatggt ttacccatgc aggtagggtt gaatacctta ctgcgtcagg
     3061 gaaaaccaga ccgcttgttg atagagccga ccgggctggg ccatccgaaa cagctcctcg
     3121 atctgttaac cgcaccagtc tatgaaccgt ggatagatct gcgcgccacc ttgtgcattc
     3181 tcgatccacg cctgctgctg gacgaaaaaa gcgccagcaa tgaaaacttc cgtgaccagc
     3241 tggctgccgc agacatcatt gtcgccaata aatccgaccg tgcgacgccc gaaagtgagc
     3301 aagcgctaca gcgttggtgg cagcaaaatg gtggcgatcg acaattaatt cacagtgagc
     3361 atgggaaagt tgacggtcat cttctggatt tgccgcgtcg caatttagcc gagttgcccg
     3421 ccagcgccgc gcattctcat cagcatgtcg tgaaaaaagg gttagcagcg ttaagcctgc
     3481 cagagcatca acgctggcgt cgcagtctga acagcgggca aggatatcag gcctgcggct
     3541 ggatattcga cgctgatacg gtattcgaca ccattggcat tctggaatgg gcgcgacttg
     3601 caccggtgga acgcgtcaaa ggcgtgctgc gtattcccga agggctggta cgaatcaacc
     3661 gtcagggcga tgacctgcac attgaaacgc aaaacgttgc gccaccggac agccgtattg
     3721 agctgatttc cagcagcgaa gctgactgga atgccttgca gagcgcgctg ttgaagcttc
     3781 gtttagcgac taccgcgtaa ggttgcctgc gtttttcagt aagataatta gagaaaatat
     3841 gattaaaaat ttgccgcaaa tagtgttgtt gaatattgtc ggccccgcgc tgtttctttc
     3901 ctggtatatt cccgttaatc atggattctg gttgccgatt gatgcggata ttttttattt
     3961 ctttaatcag aaactggtcg aaagtaaggc ctttttatgg ctggttgcat tgactaacaa
     4021 tcgcgccttc gacggttgtt cactgctggc gatgggtatg ttgatgctga gtttctggct
     4081 gaaagaaaac gcccctggca gacgacgtat cgtgattatg ggtctggtca tgctattaac
     4141 tgcagtggta ttaaaccagc tgggtcaggc attaattcct gtaaaacggg ccagcccaac
     4201 attgactttt accgatatta accgcgtcag cgaactgctc tctgttccca cgaaagatgc
     4261 ctcacgagat agctttcccg gcgatcacgg catgatgctg cttatttttt cggcattcat
     4321 gtggcgttat ttcggcaaag ttgcaggcct tatcgccctt attatttttg tggtttttgc
     4381 atttcccaga gtaatgattg gcgcacactg gtttactgac atcattgtcg gttcgatgac
     4441 cgtgatattg atcggtttgc cctgggtgtt gctgacgcca ttaagtgatc gattaatcac
     4501 cttttttgac aaatcactac caggaaaaaa caaacatttc caaaacaaat aactcacagt
     4561 aattaacatc atcagggtta tttttatagt gaggataatc ctgatgatgc gcaccgtgct
     4621 ttcatctatc gaacgcaaaa atcattctct aagtaaatga atggattgca tgcgtttcac
     4681 tcaattgtac tttaattgac caaccccgct tattaacttt ctgtatcact ttttcttata
     4741 aaaaatcatg taaaaccgct cgccaagacc gcaccaatcg ggtaatctcg aactcgtttt
     4801 gcctcggcgg tagattatcc tcacagcata taattttgtg cgttagtcca cagatttggc
     4861 cttaaggaat tgtttcaaca tgcccaggta attagtctcg tgtcgcttgg cattttttta
     4921 taacgatatt tgtcgttaag gacttcaagg gaaaacaaac aacatggtca aatctcaacc
     4981 gattttgaga tatatcttgc gcgggattcc cgcgattgca gtagcggttc tgctttctgc
     5041 atgtagtgca aataacaccg caaagaatat gcatcctgag acacgtgcaa tgggtagtga
     5101 aacatcatca ctgcaagctt ctcaggatga atttgaaaac ctggttcgta atgtcgacgt
     5161 aaaatcgcga attatggatc agtatgctga ctggaaaggc gtacgttatc gtctgggcgg
     5221 cagcactaaa aaaggtatcg attgttctgg tttcgtacag cgtacattcc gtgagcaatt
     5281 tggcttagaa cttccgcgtt cgacttacga acagcaggaa atgggtaaat ctgtttcccg
     5341 cagtaatttg cgtacgggtg atttagttct gttccgtgcc ggttcaacgg gacgccatgt
     5401 cggtatttat atcggcaaca atcagtttgt ccatgcttcc accagcagtg gtgttattat
     5461 ttccagcatg aatgaaccgt actggaagaa gcgttacaac gaagcacgcc gggttctcag
     5521 ccgcagctaa taaaccgttt ggatgcaatc ccttggctat cctgacgagt taactgaaag
     5581 cactgcttag gcagtgcttt tttgttttca ttcatcagag aaaatgatgt ttccgcgtct
     5641 tgatccaggc tatagtccgg tcattgttat cttttaaatg ttgtcgtaat ttcaggaaat
     5701 taacggaatc atgttcatac gcgctcccaa ttctggacgt aagctcctgc ttacctgcat
     5761 tgtagcaggc gtgatgattg cgatactggt tagctgcctt cagtttttag tggccaggca
     5821 taagcacgaa gtcaaatacg acacactgat tatcgacgta caaaagtatc tcgataccta
     5881 ttttgccgac ctgaaatcca ctactgaccg gcttcagccg ctgaccttag atacctgcca
     5941 gcaggctaac cccgaactga ccgctcgcgc ggcgtttagc atgaatgtcc gtacgtttgt
     6001 gctggtgaaa gataaaaaaa cattctgttc atctgcgact ggtgagatgg acattccact
     6061 caatgaattg attccggcgc tcgacattaa taaaaacgtc gatatggcga tcttacccgg
     6121 cacgccgatg gtgccgaaca aacccgcaat cgtcatctgg tatcgcaacc ctttgctgaa
     6181 aaatagcggt gtgtttgccg ctctgaatct caacctgacg ccttctctct tttatagttc
     6241 acggcaggaa gattacgatg gcgtcgccct cattattggc aatactgcgc tatctacctt
     6301 ttcttcacgt ttgatgaacg ttaatgaatt aaccgacatg ccggtccgtg aaactaaaat
     6361 tgcgggcatt cctctgaccg ttcggcttta tgcggatgac tggacatgga acgatgtgtg
     6421 gtacgcattt ttactgggcg gcatgagtgg aactgtcgtt ggcctgcttt gctattacct
     6481 gatgagcgta cgtatgcgcc ccggcagaga aatcatgacc gccatcaagc gcgaacaatt
     6541 ttacgtggcg tatcaaccgg tggtggatac acaagctttg cgggtaacgg gcctggaagt
     6601 actgctacgc tggcggcatc ctgtagcggg agaaattccc ccggatgcct tcattaactt
     6661 tgccgaatcg caaaagatga ttgtgccgct gactcagcac ctgtttgagt taattgcccg
     6721 cgatgccgca gaattagaaa aagtactgcc ggtaggcgtc aaatttggca ttaacattgc
     6781 gccggaccat ctgcacagcg aaagctttaa agcagatatc cagaaactgc tcacttccct
     6841 gcccgcacac catttccaga ttgtgctgga aattaccgag cgcgatatgc tgaaagagca
     6901 agaagccaca caactcttcg cctggctgca cttggtcggc gtagaaattg ctattgatga
     6961 cttcggcacc gggcacagcg cgcttatcta tcttgagcgt tttacgctcg attatctgaa
     7021 aattgaccgt ggatttatca acgccatcgg tacggaaacg atcacttcac ccgtacttga
     7081 cgcggtgctg acgctggcga aacgtctcaa tatgctgaca gttactgaag gggtcgaaac
     7141 gccggaacaa gcgcgatggc taagcgaacg cggcgttaat ttcatgcaag gctactggat
     7201 tagtcgcccg ttaccgctgg acgattttgt tcgctggctg aagaaaccgt atacgccgca
     7261 gtggtaaggt gtgcttacgt cccttattat tcatagtgaa agcatgccgg attgcggcta
     7321 atgatgagta aaaggaaatc cgttgcagat gattgtgcgc atactgctgc tgtttatcgc
     7381 tctgttcacc ttcggtgcgc aggcgcaggc tatcaaggaa agctatacct ttgccgtgct
     7441 gggcgaaccc cggtacgcgt ttaatttcaa ccattttgat tatgtgaacc ccgccgcgcc
     7501 aaaaggtggg cagataacgt tgtcagccct cggtaccttc gataatttca accgctatgc
     7561 actgcgcggc aacccgggcg cacgcaccga gcagctgtac gacacgttat ttacgacttc
     7621 cgatgacgaa ccaggcagtt attacccgct gattgctgaa agtgcacgct atgctgacga
     7681 ttattcctgg gtggaggtcg ctattaatcc gcgcgcccgt tttcatgatg gttctcccat
     7741 tactgcccgc gatgtagagt ttacttttca aaaatttctg accgaaggcg tgccgcaatt
     7801 tcgtctggtc tacaaaggca ccaccgtcaa agccattgcg ccgttaaccg tgcgcattga
     7861 gttagctaaa cccggcaaag aagatatgtt gagtctgttt tcgctgccgg tatttccaga
     7921 aaagtactgg aaggatcaca aacttagcga cccgctcgcc acgcctccgc ttgccagtgg
     7981 tccgtaccac gttacgtcct ggaaaatggg gcaaaatatt gtctattccc gcgtgaaaga
     8041 ttactgggca gcaaacttac cggtaaaccg tggacgctgg catttcgaca ccattcgcta
     8101 cgattattac ctcgatgata atgtcgcctt tgaagcgttt aaagcaggtg cctttgattt
     8161 gcgtatggaa aacgacgcta aaaactgggc cacgcgttat accggtaaaa atttcgataa
     8221 aaaatacatc atcaaagatg agcaaaagaa cgaatcagcc caggatacgc gctggctggc
     8281 gtttaatatc caacgtgcgg tattcagcga tcgccgggtg cgggaagcaa tcactctcgc
     8341 ctttgacttt gaatggatga acaaggcgtt gttttacaac gcctggagtc gcacgaacag
     8401 ttattttcaa aataccgaat acgcggccag aaattacccc gacgccgcgg agctggtgct
     8461 tctggcacca atgaaaaaag atctaccgcc agaagtcttc acacaaatct accagccgcc
     8521 ggtatctaaa ggcgatggct acgatcgtga caacctgtta aaagccgaca aacttctcaa
     8581 cgaagcgggc tgggtgctga agggtcagca acgcgttaat gccacaacgg gtcagccgct
     8641 cagctttgaa ttattgcttc ccgcaagcag caatagtcag tgggtattgc cgttccagca
     8701 cagcctgcaa cggctgggta tcaatatgga cattcgcaag gtggataact cgcaaatcac
     8761 taaccgcatg cgcaatcgcg actatgacat gatgccgcgc ctatggcggg cgatgccgtg
     8821 gcccagttcc gatttacaga tttcctggtc atcggaatat atcaattcca cttataatgc
     8881 ccccggcgtg caaagcccgg ttatcgactc gctgatcaac caaattattg ccgcgcaggg
     8941 aaataaagaa aaattactgc cgttggggcg agcactggat cgcgtattaa cgtggaatta
     9001 ttacatgctg ccaatgtggt acatggcgga agaccgtctc gcctggtggg ataaattctc
     9061 ccaacccgct gtacgccctg tttacagcct aggtatcgat acctggtggt atgacgttaa
     9121 taaagcggca aaactgccgt cagccagaca acagggagag tagatgggcg cttacctgat
     9181 tcgccgtctg ttgctggtga tcccaacgtt atgggctatt atcactatca actttttcat
     9241 cgtgcaaatt gcgcctggcg gtccggtcga tcaggccatc gccgccattg agtttggtaa
     9301 tgccggagta ttacccggcg caggcggtga aggtgttcgt gccagccatg cgcaaacggg
     9361 tgtcggcaat atcagcgaca gtaattaccg tggcggacgc ggattagatc cagaagtgat
     9421 cgctgagatc actcatcgct acggtttcga taagccgatc cacgaacgtt acttcaaaat
     9481 gctctgggac tacatccgct ttgattttgg tgatagcctg tttcgcagcg cgtcggtgct
     9541 gacgctgatt aaagacagtc tgccggtttc catcaccctc ggattgtgga gcacgctgat
     9601 tatctatctg gtgtcgattc cgttaggcat tcgcaaagct gtttataatg ggagtcgctt
     9661 tgacgtctgg agtagcgcat ttatcatcat cggctacgcc attccggcct ttttgtttgc
     9721 catcctgctg attgtcttct tcgcgggcgg cagctatttc gacctgttcc ctctacgcgg
     9781 cctggtttcc gctaactttg attcgctgcc gtggtatcag aaaatcaccg attatctgtg
     9841 gcatatcacg ctaccggtgc tggcgacagt gattggtggc tttgcggcgc tgaccatgct
     9901 gaccaaaaac tcattccttg atgaagtgcg caagcaatac gtggtgaccg cgcgtgcgaa
     9961 aggggtaagt gagaaaaaca ttctctggaa acatatgttc cgcaacgcca tgctgctggt
    10021 gattgccggt tttccggcga cgtttatcag catgtttttt accggctcgc tgctgattga
    10081 ggtgatgttt tcactcaatg gtcttggctt actgggctac gaagcgaccg tctcgcgcga
    10141 ttatcctgta atgtttggta ccttgtatat tttcaccctg attggcctgc tgctgaatat
    10201 tgtcagtgat atcagctata cgctggttga tccgcgtata gattttgagg gacgttaatg
    10261 tcgcgactca gccccgtcaa tcaggcccgt tgggcgcgtt ttcgtcataa ccgtcgcggc
    10321 tactggtcgt tatggatttt cctcgttttg tttggtttga gtttgtgttc tgaacttatc
    10381 gctaacgata aaccgttgct ggtgcgttat gacggcagtt ggtatttccc gttgttgaaa
    10441 aactacagcg aaagcgattt tggcggcccg ctggcaagtc aggctgatta tcaggacccg
    10501 tggctgaaac aacggctgga aaataacggc tgggtactgt gggcaccgat tcgctttggt
    10561 gctaccagta tcaactttgc taccgataag cccttccctt ctccaccctc ccggcaaaac
    10621 tggctgggaa cggatgcaaa cggcggcgat gtgctggcac gcattctcta tggcacgcgg
    10681 atctcggttc tgtttggcct gatgctgact ctctgttcca gcgtgatggg cgtgctggcg
    10741 ggggcgctac aaggctatta cggcggtaaa gtcgatctct gggggcaacg ctttattgaa
    10801 gtatggtcgg ggatgccgac gctgtttttg attattttac tttccagcgt cgtacagcct
    10861 aacttctggt ggctgctggc aattactgtc ttgtttggct ggatgagtct ggtcggcgtg
    10921 gtgcgggcgg agtttttacg tactcgtaat ttcgactaca ttcgtgcggc acaggcgctt
    10981 ggcgtcagcg atcgcagtat catcctgcgt catatgttgc ctaatgccat ggtcgcgacc
    11041 ctcacctttt taccgtttat tttatgtagt tcgataacca ccctgacctc gctcgatttc
    11101 ctcggcttcg gtctaccgct cggttcaccg tcactcggtg aactgctgtt acaagggaaa
    11161 aataaccttc aggccccgtg gcttgggatc accgccttct tgtcggtggc gatattattg
    11221 tctttgctga tctttattgg tgaagccgtc cgcgacgcat ttgatcctaa taaggcggtg
    11281 tagcatgacg caaactctgt tagcgattga aaatttgtcg gtgggttttc gccatcagca
    11341 aaccgtacgt acagtagtca atgatgtttc actacagatt gaggctggcg aaacgctggc
    11401 gctggtgggt gagtcaggtt caggcaaaag cgttaccgcg ctgtcaattt tacgcctgct
    11461 cccttccccg ccggttgaat atctctccgg cgatattcgt tttcatggcg aatcgctgct
    11521 tcatgccagc gatcaaacgt tgcgcggtgt acgcggtaat aagatcgcca tgatttttca
    11581 ggaaccgatg gtgtcgttaa atccattgca taccctggaa aaacagcttt atgaagtgct
    11641 ttcactccac cgcgggatgc gtcgggaagc ggctcgtggc gaaattctta actgccttga
    11701 tcgcgtcggt atccgccagg cggcaaaacg gctgacagat tatccgcatc agctctccgg
    11761 cggcgaacgc cagcgggtga tgattgcgat ggcgctgtta acgcgaccgg aattattaat
    11821 tgccgatgaa ccgaccaccg cactggacgt ctctgtccag gcgcagattt tacagctgtt
    11881 gcgcgaactg caaggcgagc tgaatatggg catgctgttt attactcata acctcagcat
    11941 tgtcagaaaa ctggcccacc gcgtggcggt aatgcaaaac ggtcgctgtg tcgagcaaaa
    12001 taacgccgct acgctatttg cctcacccac tcatccttac acacaaaagc tactcaacag
    12061 tgaaccgtca ggcgatccgg taccgttgcc agaacctgcc tcaacgttgc tggatgttga
    12121 acagcttcag gttgccttcc ccattcgcaa agggattttg aagcgcattg tggatcataa
    12181 tgtggtggtg aaaaacatca gttttacgct acgggcgggt gaaacactgg gtttagtggg
    12241 cgagtctggt tccgggaaaa gcacgacggg actggcgctg ctgcgactga ttacctctca
    12301 gggcagcatc gtctttgacg gtcagccact gcaagattta aatcgccgcc agctgttacc
    12361 tactcgtcat cgtattcagg tggtatttca ggatccaaac tcctcgctca acccacgact
    12421 caacgttttg cagattattg aggaaggctt acgggttcac cagccgacgc tttctgccgc
    12481 acaacgcgaa caacaagtga tagccgtgat gcatgaagtg ggattagatc ctgaaacacg
    12541 ccaccgttat ccggcggagt tctctggtgg tcagcgacaa cgtattgcga ttgccagggc
    12601 attaattctt aagccctcgc tgatcatact tgatgaaccg acatcatcac tcgacaaaac
    12661 ggtacaggcg caaatattga cgcttttgaa atcattgcaa caaaagcatc aactggccta
    12721 tttgtttatc agccacgatt tgcacgttgt ccgcgcgtta tgtcatcagg ttatcgtact
    12781 gcgacaaggg gaagtagtgg aacaaggacc gtgcgcgcgc gtgtttgcca caccgcagca
    12841 ggagtatacg cgtcagttac tggcgttgag ctgacgctta aaaaggattg tagtttgaaa
    12901 agggttcggc aattgccaca ccaaaatttt tcaatcggca tgttgcagca aactcgtcct
    12961 ggcgatttac aaacaggcac ggctcacctt cacactccaa caccgaacat ggaacttcga
    13021 tgtcgcttaa cgcctggctt agtttataca tcaccgacca tgcattatca ccatccgggc
    13081 taagcaattt aagcgccacc aggctgttgt caccgcacgg tccattttgc ggaatcggtt
    13141 caaccttcga acctgcgaaa ccagcagacc agcgatagtt ctgcggcagt cgatggacga
    13201 ttgagagttg taatgaagtc acttattttc cccggaagta cattcacttc acaatttgtt
    13261 tacatcaatt ttaacacatc atcaacaatc cgtctttata cagatcgtaa agattgatgc
    13321 ctcgcctgcg tggcctctat ggctctgatt taagtaatat ctgcgggcgt ggtcacgttt
    13381 gcggggctat atgtacccgt ttctgcgatc taactcaacc tttttaacta caatgatgtg
    13441 actttttaca taaattgatt ttacataaaa taaacatata tcggggaaat gatagatttg
    13501 tggttgacat gcatcaacaa aggaagcctt ttaccttcct cgttgtgcaa tagatcaccg
    13561 ttttttcggc cgactggcgt acagacagaa gagaatggag ctggttgcgc agaatgcaat
    13621 tgaccaaatc atcggccatg cagagttaaa ggtcgcaaga gaaagcaatg cgccaacaat
    13681 tgcccctatg ccaaaacgga aggttcctgc cagcgaagat gccgttcccg ccatatgggg
    13741 aaactcatca agaatgaccg ccatcgcatt ggatgacacc atcgacacgc agcccacaaa
    13801 caccgcaacg ccaaccacca gcgaccagaa tcccagcccc agcagcgcac tgatgaccat
    13861 ccacgctgcc ataataaatt gtatccacaa ccccgagcgg aacatattta acgcgccaat
    13921 gcggcggacg aagcggctgt taaagatggt catcacgaac agaaaaacaa tgtttagcgc
    13981 aaagtaataa ccaaagtttt ccggcgcgac gtggttaatt tcaatataaa caaacggtcc
    14041 ggcgcttaag aatgagaaca tcccggcaaa gctgaaacca ctggcaagca tgtagctcag
    14101 gacacgttta tggcggaaca gcgccgcaaa gttaccaata gtggtacgaa tgtgaaatgg
    14161 ctgacgacgc tccggtggta aggtttcttt aatcaggaag aaaatcattg ccgaagccag
    14221 aatcgccgct aatgccagga tccagaagat gtaatgccag ctcagccaca ccagcaccca
    14281 gccgccaact atcggtgcca tcagcagtgc aatggtcgtc accagcatga caaacgacat
    14341 catccgcgag aactcttctt tcgggtaaat atcacgcatc aaagcgttga taaccacgct
    14401 ggccgcagcc gcagccagcc cgtggaagaa acgcatcaca atcagctgat cgatggtttg
    14461 tgccaacgca cacgccaccg cggcggcggc aaacaccagc gtcccgccaa gaaccaccgg
    14521 cttacgcccg aagctgtctg ccatcggccc gtagattaac tgccccaacg caaagcccag
    14581 aatataagta ctgagggtca tctgcgtact gcccgccgat acgccaaact gcgctgaaat
    14641 taccggtagc gcgggcagat acatatcaat cgacagcggc atcaacatgg ccagcaggcc
    14701 aaggataaaa acaatagcaa acgacgaatg ctgtcgggtg gtcacaacgg gctcctgaaa
    14761 gtcattgaaa agttagacga cgctggcaat ttcttcttca gttaacggac gatattcacc
    14821 gggggctaaa tcagcatcca gcgtaatacc gccaatacgt tcacgatgca gctcaaccac
    14881 gtggttaccc acggcggcga acatgcgttt cacctgatga taacgccctt cgctgatggt
    14941 cagacgaacc tgcgttgggg taatcacttc aagcaccgcc ggcttagtga gatctttttc
    15001 gttatgcagc tgcacgcctt tagcaaattg ctctgccgta tcgtcagcta caggtgattc
    15061 cagtgtcacc agataggtct tctcgcaatg atggcgcgga gaagtaatgc ggtgcgacca
    15121 ctgaccatca tcagtcatca gcaccagacc ggtggtatcg atatccagac gtcctgcggc
    15181 gtgcagtttc cacgctaccg gttcatccag aaaatagagc accgtcgggt gatccgggtc
    15241 gtccgtggag caaacatagc cctgaggctt attgagcatg aagtaacgtg gaccgtgttg
    15301 ctgcgccagc gggttgccat cgtaagcgac atcatgttca gggagcagtt tgaacgctgc
    15361 attacgaacg atttcgccat cgacggtgac acgattgccg cggatttcac gcccggcaat
    15421 agcacggcta acgccgagtt gctgtgcgat aaatttatca agtcgcatgt gtgtgatttt
    15481 gcctgtaaaa aacggaggtc gggtaatgac ccgaaaatct gaactgttgt ctgcccagta
    15541 tagcggtcta ataacttccc tcaagggaaa aagaaccgtg gcatactatt agcagaataa
    15601 tctacctacg cgaaaccatg atttttacac tccgcccata tcagcaagaa gccgtggatg
    15661 ccacgctcaa ccattttcgt cgtcataaaa cccctgccgt tatcgtgctg cccaccggcg
    15721 caggtaaaag cctggtgata gcggaactgg cacggctggc gcgtggtcgc gtactggtgc
    15781 tggcacacgt taaagaactg gtggcgcaaa accatgcaaa gtatcaggcg ctggggctgg
    15841 aagccgatat ttttgccgcc gggctaaagc gcaaagagag ccacggtaaa gtggtatttg
    15901 gcagcgttca gtcggtcgcc cgtaatcttg atgcctttca gggtgaattt tcgctgttga
    15961 ttgtcgatga atgccaccgt attggtgacg atgaagagag ccagtatcag caaatcctca
    16021 ctcacctgac taaagtgaat ccccacttac gcctgctggg gctgactgcc acgccctttc
    16081 gattgggcaa aggctggatc taccagttcc attatcacgg catggtacgc ggcgatgaga
    16141 aagccctttt ccgtgactgc atttatgagc tgccgctgcg ttatatgatt aaagacggct
    16201 atctgacgcc gccagaacga ctggatatgc cagtagtgca atacgatttc agccgcttgc
    16261 aggcacagag taacgggctg ttcagcgaag ctgatctcaa ccgtgagctg aaaaaacagc
    16321 aacgcattac gccacatatc atcagccaga tcatggagtt cgccgaaaag cgcaaagggg
    16381 taatgatttt cgccgccacc gttgaacacg caaaagagat tgtgggatta ctgcctgccg
    16441 aagatgctgc actgattact ggcgaccccc ccggcgctga gcgcgatgtg ttaattgaag
    16501 attttaaagc ccagcgtttt cgctatctgg tcaacgtcgc agtactgacc accggatttg
    16561 acgccccgca cgtcgatctt atcgccattc tgcgccctac cgaatcagtg agtctttacc
    16621 aacaaattgt cgggcgcggt ctgcgtctcg ctccgggcaa gactgattgt ttaattcttg
    16681 attatgcggg taatcctcac gatctctacg ctccggaagt tggtacaccg aaaggcaaaa
    16741 gtgacaacgt tccggtacag gttttctgcc ctgcctgcgg ttttgccaac accttttggg
    16801 ggaaaacgac cgccgacggg acattgattg aacactttgg tcgccgctgt cagggatggt
    16861 ttgaagatga cgacggtcat cgcgaacagt gtgacttccg tttccgtttt aaaaattgcc
    16921 cgcaatgtaa cgcagaaaat gatattgccg ctcgccgctg ccgcgaatgt gacacggtac
    16981 tggttgatcc ggatgatatg ttaaaagcgg cgctacgact gaaagatgcg ctggtattac
    17041 gctgtagcgg catgtctttg cagcatgggc acgacgagaa aggcgaatgg ttgaaaatca
    17101 cctattacga tgaagacggc gcggatgtga gtgagcgttt ccgtctgcaa acgcccgccc
    17161 agcgtaccgc cttcgagcag ctttttatcc gcccgcatac gcgcacaccg ggcatccctc
    17221 tgcgctggat caccgccgcc gatatcctcg cccagcaagc cttactgcga catccggatt
    17281 ttgtcgtcgc ccgcatgaaa ggtcagtact ggcaagtgcg tgaaaaagtg ttcgattacg
    17341 aaggtcgttt tcgtcgggcg cacgaattac gcggttaatg gtagttttga ttgatgtata
    17401 aggcgattga gtatagaatc tcgcccgctt ttgcatacgc aaagcagatc acttacctgt
    17461 tgctgggtcg cctgtagcag gattaattta agagagaaag aaatgtttac tatcaacgca
    17521 gaagtacgta aagagcaggg taagggtgcg agccgccgcc tgcgtgccgc taacaagttc
    17581 ccggcaatca tctacggtgg caaagaagca ccgctggcta tcgagctgga tcacgacaaa
    17641 gtcatgaaca tgcaagctaa agctgaattc tacagcgaag ttctgaccat cgttgttgac
    17701 ggtaaagaaa tcaacgttaa agctcaggac gtacagcgtc acccgtacaa accgaagctg
    17761 cagcacatcg acttcgttcg cgcttaattg ctgaataagt tgtaaaaaac cccgctccgg
    17821 cggggttttt tgtatctgca gattatgcct gattacggta ttgctatttt ttgcaggcca
    17881 gataaggcgt tcacgccgca tctggcattc agaacacaaa accgattaat tgccgccaga
    17941 cgtccggcgc tgcaattggt cgcgcaaatt cggcggcgtg cctttaatgg tcaaggtatc
    18001 ggttgctgga tcccagaaga tgcgttcacc taacagcatc gcatcaaagt taatagtcaa
    18061 gccaccgccg ctaccggcaa acttcgtcaa ctgacgcagt gtgctacggt ctgccgggaa
    18121 gctctcttcc agttcgtagc ctttttcagc cgcaaactcc gtgaagctca cttcgcttac
    18181 gcctgccagc tctttcgaca gcgattccag ttcgatctct tcgcctgcct gcagctgctc
    18241 attacagtag ctataaacct gctggcgcac gttctgtcgc tctgctttat ccagttgtgc
    18301 ttcggcagtg aaatcatcaa ccgcttgcaa cagcccacgg ttttgcgctt tcgcgttcaa
    18361 tccttcgctg gccccgagga aatccataaa gaaatccgcc actttgcgcc ctactcgccc
    18421 tttcaggaaa gtgagatagc gggtggactc tggattggtt tcccattcgg ttaaatcgat
    18481 acgcgcaaca atatccgcat gattgatatc aagataatgg gttgggttga tgtcgagatt
    18541 ttcgttaacg cgcatactgc tcaggttgct cagaactgcc accagcagat actccaccgc
    18601 cagataacgg tagtggcaaa acagcacgaa tccaccgtca gcaaaaggat atttcgctaa
    18661 ttcgtcacgc aggcgaccgg ttgccgcgcg gctaaatgcc agaaagtcct cttcgccctg
    18721 acgctgtaag cgcagcgtct gcgccagttc actctcttcg ctaaacaagc cgtaagcttt
    18781 atttttggcg ctatagaccc gatgcagttc tgccaccatc tcaacgacgg tttctgtcgg
    18841 ttccagcaat gaatcgcgca acaccagctc aaggttctgc tcatcacgct tgataagctg
    18901 gtgcagagca atctggttga tatccagact catgataaac tctcctttaa gaccgggcgg
    18961 tattcaacca ccgccggtta agcgacgcaa taaaagatgg cggtcatttt acagaaggca
    19021 gcgtaacgag aatgatttaa cgcataaaaa agcagaaaaa aaaccgttgc tacggtaata
    19081 tgttgccctt tcatcaacta actgatttcg atttatgcca caaatttccc gctacagtaa
    19141 tgaacaggtt gaacaattgc tcgcagagct gctcaacgta ctggaaaaac ataaggctcc
    19201 gaccgacctt tccctgatgg tgttaggcaa catggtcact aaccttatca acaccagcat
    19261 tgccccggcc caacgccagg caattgccaa ctcttttgcc cgcgccttac agtcctctat
    19321 caacgaagac aaagcgcact aagggaaaca gataacaggt tatggtaact catcgtcagc
    19381 gctaccgtga aaaagtctcc cagatggtca gttgggggca ctggtttgca ctgttcaata
    19441 ttctgctttc gctcgtcatt ggcagccgtt acctgtttat cgccgactgg ccgacaacgc
    19501 ttgctggtcg catttattcc tacgtaagca ttatcggcca tttcagcttc ctggtgttcg
    19561 ccacctactt gctgatcctc ttcccgctga cctttatcgt cggctcccag aggctgatga
    19621 ggtttttgtc cgtcattctg gcgacggcgg gaatgacgct attactgatc gatagcgaag
    19681 tctttactcg tttccatctc catcttaatc ccatcgtctg gcagttggtt atcaacccag
    19741 acgaaaatga gatggcgcgc gactggcagc tgatgttcat cagcgtgccg gttattttat
    19801 tgcttgaact ggtgtttgcg acgtggagct ggcaaaagct gcgcagcctg acgcgtcgtc
    19861 gacgcttcgc gcgcccgctg gccgcattct tatttatcgc ctttatcgcc tcgcatgtgg
    19921 tgtatatctg ggccgatgcc aacttctatc gcccgatcac catgcagcgc gctaacctgc
    19981 cgctttcgta cccgatgacg gcgcgacgtt tccttgagaa gcatggcctg cttgatgcgc
    20041 aggagtatca acgccgtctt attgagcaag gtaatccaga caccgtttcc gtacagtatc
    20101 cgttaagcga actgcgctat cgcgatatgg gcaccgggca gaatgtgctg ttgattactg
    20161 tcgatggcct gaactactca cgcttcgaga agcagatgcc tgcgctggca ggttttgctg
    20221 agcaaaatat ttcgttcacg cgccatatga gctccggcaa cactacagac aacggcatct
    20281 ttggcctgtt ctatggcatc tcgccgagct atatggacgg cattctgtcg acccgtacgc
    20341 ctgcggcatt aattactgcg cttaatcagc aaggctatca gctggggtta ttctcatcag
    20401 atggctttac cagcccgctg tatcgccagg cattgttgtc agatttctcg atgccgagcg
    20461 tacgcaccca atctgacgat cagaccgcca cgcagtggat caactggctg ggccgctacg
    20521 cacaagaaga taaccgctgg ttctcgtggg tttctttcaa tggcactaac attgacgaca
    20581 gcaatcagca ggcatttgca cggaaatata gccgggcggc aggcaatgtc gatgaccaga
    20641 tcaaacgcgt gctcaatgca ctgcgtgatt ctggcaaact ggacaatacg gtggtgatta
    20701 tcactgccgg tcggggtatt ccactgagcg aagaggaaga aacctttgac tggtcccacg
    20761 gtcatctgca ggtgccatta gtgattcact ggccaggcac gccggcgcag cgtattaatg
    20821 ctctgactga tcataccgat ctgatgacga cgctgatgca acgcctgctg catgtcagca
    20881 cacctgccag cgaatattcg caaggtcagg atttgttcaa ccctcaacgc cgtcattact
    20941 gggttactgc tgcggataac gatacgctgg caattaccac cccgaaaaag acgctggtgc
    21001 tgaacaataa cggtaaatac cgcacttaca acttacgtgg tgaaagagtg aaagatgaaa
    21061 aaccacagtt aagtttgtta ttgcaagtgc tgacagacga gaagcgtttt atcgctaact
    21121 gattaattat aaatcagtta gcgaaatatc ttacttgcaa tcggtgtgga aaacggtagt
    21181 attagcagcc acgagtcggc acgtagcgca gcttggtagc gcaccgtcat ggggtgtcgg
    21241 gggtcggagg ttcaaatcct ctcgtgccga ccaaaaatcc caagaaaaaa ccaaccctta
    21301 cggttggttt ttttatatct gcaattaatt cgataaacag accgtgacac atcacagcct
    21361 gtttattttc tgttatcaga acgtccagac cacacccgcc tgagtattcc acggcgattc
    21421 tacccctgcg ccattgccat agctgactga caaatgaccg cttaacgtcg gggtaaacga
    21481 tgaccttatc ccagcctgat aaacgccact ggttccagcc acatcattat taaatttgcc
    21541 atcgtcatta actttcacct ggttagaatc ggcgtattcc tgacgcacgg cggctttcag
    21601 ccagggttcc agcgtcgtac cgttttgcag gtccatgtga tagcttaccg ccgttcccgc
    21661 ttcagcgcgt aatatccggg tatttcccac atccgcgcgc atgccgtttg ataacgtgta
    21721 gtcctgacca tctgtggtaa agccggtaaa ggccagatag ggtctaacac tccacaatcc
    21781 gtcaacccaa cggaacccgc tctcaacatg agcacccgcg ccgttactat tgtaatcgcc
    21841 aaacgctgtt gccccattac tcatcttgcc atggatggtg ttggcaaaac ggtcaacttt
    21901 caccacccca tcaacatagg caccgttctg atgctcccaa ccggcataag cccccagggt
    21961 atagctatcg atattacctt ttccgccgcg atcaaaacca atatcagaat gagagtaacc
    22021 aaagaacaag ccgcgaattg tactgctttc ttcacgggag aaacggctat cgataccgag
    22081 cgtcaggccc gtcaatgttt gctcaaaacc agctcccgca tcagtggtca cgttgttgcg
    22141 ggtgttaatt gccgaactcc acatcaccgt atcgtaacta acgcctttta cgctaccaag
    22201 acgctcacgc acggtgtcca gttctgcatc aaataccagc ggttgtgcgg ctgccatgtt
    22261 cagcacatca gtggttgaag gggtaatttg cgcgcgattc tctgcgaggc tccagctatg
    22321 gttgccatta tccagcaagg tatattcata cgtaccgata tcaacaacgc ctccggcatt
    22381 gcccaacgta aatgcagcat caccgccgcc cgttgttacc agtgtaaggc tatctcctgc
    22441 tgccgggctg gcaccggtgt ccgtcacgaa tattttgaaa tcacctgttg cctgaccggt
    22501 gacgttgagc tgatcgctct gatgattggc catatcggta cgcatataaa aattgccgtt
    22561 tcccgacagg gtattggtgg tcaacgtaat gaaattttcc gcacttgccg tcacagatga
    22621 acgagtcact ggctcagcca tcataatcac atttgcgccg gacatcgcaa ggttggctaa
    22681 gttgaggcga gtgttttggt tcgtcgtagt gatatcggca atgatggtcc atgcgctatt
    22741 ttcgagcgaa acgtccgctt tgctggtatc cacggcacca tgcgttctaa aagaagcgcc
    22801 ctcagaaacc actaccttat ccaccaacat agcgggtgca taatttattt gcggcttcat
    22861 gacctcaaga atgccatcat tccctctgac tgacacgtta accattgtgc cagcccagac
    22921 attagcgcgg ccattgttga ttaccatatt ttcagagatg gcttttgagg aatacgtgat
    22981 acttccattc tggtctgatc gaccgagatc atataccgca tttgcctgca ctttcgtacc
    23041 agtatccttt cccagcgatt gcatagtggc atgctcatca aggatagtgt caatggcctg
    23101 cgtgtcctcc ataacaatga gcccggaacc atcatccagt tcataatttt ttgacacacc
    23161 atcctttata ctaaattggc ctttactgtt tgtaccactc acttccagcg cattcgttga
    23221 gacaattaat ttcccgcctg cttttttatc aacgccagtt gcagtcccgc catccataac
    23281 ctccagtaag ccgccactat ccacagtagt attataagcg aagtcatttt cttcaactcg
    23341 caaacttccg ccgttttcca acaacatatt attagcaata ccattcttga tatcgaactg
    23401 accgagacgg tttgttccga atacctccat ggcccgcgtg gttgttttaa tagcaccgcc
    23461 tgctttctga tctaccgcaa gcgcaaatcc cccctcttta acctccagta gtcctccctt
    23521 ttcgatagcg gcaaaaacgg tccccccatc tttttcaacg attaagcgcc cgcctttaat
    23581 cgtggtatca ttcgctgggc cataaacctg tagcacacca ttattgaggg tagtctggtt
    23641 tgccgcgcca cccatttgaa caatttgata accatcattt aatactgagt attctgcggt
    23701 accattcatt tcaatgtatt gataccctcc gttaatttca gtattactac ttacaccaaa
    23761 ttcttttata tgctgttctc cgccaggatt gattttcgta ttattggcgg tcccataaac
    23821 cagctggata tctttttcca ggataacacc atcaactgtc tcaccatact caactgttga
    23881 tgccaatgct atgggtgaat ataacgctgt agccaccaga acagatattg cggaacgaca
    23941 aagagaaaca gaacaagatt gatgcattga gctttcatcc tatgaaatta attgcagtta
    24001 aaagcattgg gtacagaaaa tacccatagc tccatacccg gagtcagttt ttaaaaactg
    24061 tttaaagaaa tgcacaagta ttgtgattga ttttttagtt gtttttcttg atgagaagct
    24121 gatgcaaaat tccgtcttta taatgaaaat gatgccaaag cgaacgacaa ggttgtagtt
    24181 ttcactacat gtccatacat aaaatggggt aacattcacg cgcctggtag cgttaccaac
    24241 gctacgctca aacataatga ttctaataaa acctcaggag actactatgc ctgaagcaac
    24301 gccttttcag gtgatgattg tggatgatca tccacttatg cgacgcggtg ttcgtcagtt
    24361 actggagctt gatcctggct ttgaagtggt cgccgaagcg ggcgacggcg cgagcgcgat
    24421 cgatctggcg aatagactgg atatcgacgt gatcttgctg gatctcaata tgaaaggtat
    24481 gagtggcctg gataccctca atgccttgcg cagggatggc gttaccgcgc aaattattat
    24541 cctgaccgta tccgatgcct ccagcgatgt ctttgcgctg atagacgcag gcgcagacgg
    24601 ttatctgttg aaagacagcg acccggaagt attgctggaa gcgattcgta ccggagcgaa
    24661 aggcaacaaa gtctttagcg aacgcgtcaa tcagtactta cgtgaacgtg aaatgtttgg
    24721 cgcggaagaa gatcccttca gcgtgctgac ggagcgcgag ctggatgttc tgcacgagct
    24781 ggcacagggg ctgtcaaata aacagattgc atcggtgttg aatatttccg agcagacagt
    24841 aaaagtacat attcgcaatc tgctgcgtaa actcaatgtc cgctcacgcg tggcggccac
    24901 cattctgttc ctgcaacaac gcggggcaca ataaaaatag cccgatggat ttatcatcgg
    24961 gctgagattt atgacaaacg caaaactgcc tgatgcgcta cgcttatcag gcctacgtgg
    25021 atcgatcaat ttattgaatt tacacaattt tgtaggccgg ataaggcgtt cacgccgcat
    25081 ccggcataaa ctaagcgcac tttgtcatca gtctagcccg atggcatcac catcgggcct
    25141 ctttttattt actctcctgc ggcgacaaat gttgcatcgc ctgcgcgata ctacgttcaa
    25201 tcaccgcacg gcgagtatcg ttggcaggta agagtttcaa catcatctcc cacgcggcaa
    25261 cggcttcgcc aaatcgctgc tgctcaaagg cattaaacgc atacatgctt agcacacgga
    25321 tattgctatg gtccgttctc accagctgac gtagcagttc accgccgagg cggttgtcgt
    25381 tggtatcaga tgaacgagtc aacgcttcag cgtatcccag tgcggcatcg ctgttcttcg
    25441 gatcgaggcg atacgcagta gcgtatgcat cggtggcgat actggcgtta cccagcgcca
    25501 tgccaacgcg gcccaacata atccagcctt ctatatctcc cggatttttt tgcagttgag
    25561 tacgcatccc cagcgcaagg cgcgacatct cttcttcgtt gagcggatcg gctttcgggt
    25621 ccagcgccct gtccagcaac gccggagcct gtgccgtggc ctgctgccag attttcacct
    25681 gctgataatt gccagtctgg tagtagctga cgccagccac aattaacgcc accacaatac
    25741 ccggcagata aacaacatat ccggcacgct taccttccgg cacgctttgt tcaggaaacg
    25801 cttccggcac cacgcgtacc cgacgccgcg agcgggcgta tatgacccaa ccgccaatgc
    25861 caatagccac tactggcagc acccacagca gcacggtcag cggcgttaac ggcggatcgt
    25921 aagtgacgaa gttgccgtaa cgcgccacca tataatcgac aatctctttt ttacttttac
    25981 cttcctgcat cagttcatac actttctgac gcaggtcggt ggcaatcatc gagttggaat
    26041 cggcaatgct gttgttctgg catttcgggc agcgcagttc ttcagtgagc tgacggaact
    26101 gttgttcctg tgcttcatct ttaaactgca acacgtcgat ggtcgccagc gctgagccgg
    26161 agatcatcag catcagcacg cccaataaaa acctcattgt gcggcctcct tactgtattt
    26221 ctcccacagc ggcttgatct cttcttccca gacgcgagga ttgagatcgc ccgcatggcg
    26281 atagcgaatg ataccgttgc cgtcaataag gaaggtttct ggcgcgccat agacaccgag
    26341 atccagccct aacatgccgt cgccatcaaa caggcttagc gcgtaaggat tgcccagctc
    26401 tttcagccag ctgattgcct tctggcgatc gtctttatag ttcatgccga ccacgcgaat
    26461 gccctgcgct gaaagctgat tcagatattg atgttccgca cggcaggtcg gacaccaagt
    26521 cgcccagacg ttaagcagta ctggtttgcc ctgagtcagc acatccgcct gataaaactg
    26581 ccccggattg tccagtgatt cgagacgaaa cttcggcaca ggcttgccaa tgagcgccga
    26641 ttccagattg gtcggatcat ccccttcggc attacgcgcc agctgccaca gcagcgccgc
    26701 ggcaatcgcc aggaagataa tcaacggaat taacaatact ttgcgcttca tacggcctcc
    26761 ggcgcagttt tttgcggact cacgcgctta cgatagcgag gatcaaacag acacagcagt
    26821 ccgcccaacg ccatcatcag cccgcccgcc caaatccagc gaacaaatgg tttgtagtaa
    26881 agacgcacgg cccacgcgcc gttttccagc tcttcaccga gcgcggcgta caggtcacgc
    26941 gtgatgccgc cgtcaattgc cgcttcggtc atcatcgacc cggcagtgtt gtaataacgt
    27001 ttttccgcat acagcaccgt ttccggttta ccctctcgcg ttacgccaat agtcgccaca
    27061 ccgccgcgcc agttcgggcc agtcacctct ttgacatcac ggaaggtgaa gcgatattca
    27121 tgaatatcga cgctatcgcc ggacttcatg cgcacatcac gctcaacgct ataattctgg
    27181 ctaaaggcaa tgccaacaat tgtcactgcc agccccaggt gagccgccac catcccccaa
    27241 taactgaagg tggttttcgt gccgcgtgaa atacgcagcg cagcttccgc agttgccagc
    27301 accgcaatcc agcaggccat tgccaggccg agcaccgtca tcgccacaac tttgctttcg
    27361 aacagccacg gcaacagcag agacagcacc agcgtagaga tgaaggcgat aatcaataaa
    27421 ttgcggattt tacgtgggcg atcgcgcccc cagcgcacca gaggaccgac accaagcagt
    27481 agcgcaaacg gcaccatcag ccaggtaaac atggtgttga agaacggttc gccaatcgaa
    27541 atactgccca gtccaagttg cttgtgcacc agcggcagca gcgtccccag caacaccacc
    27601 agcatcgcgg cgaccagcaa aacgttgttc gctaacagca aagattcccg cgaccacagc
    27661 gcattgttta cgcgtgagcg aactttgtgt ccacgcgcgg caaacagcag cagcgaaccg
    27721 ccaatcacca gcaccataaa ggcgaggata aacataccgc gcgccggatc agacgcaaac
    27781 gcgtgtaccg ataccagcac gccggaacgc acgaggaaag tccccaacag acacaacgag
    27841 aaggcactga ttgccagcag taatgtccac gctttgaagc tggcgcgttg ttcagtgacc
    27901 gccagtgagt gcatcagtgc agtccccacc agccacggca taaacgaggc gttttctacc
    27961 ggatcccaga accaccagcc accccagccg agttcgtaat aggcccatgc ggaaccgagc
    28021 acaatgccga gcgtcaggaa aatccacgcc gccagcgtcc acgggcgagt aaaacgcgca
    28081 taagtgctgt ccagacgccc gctcagcaaa gaagcaatgg caaaagcaaa cgccaccgag
    28141 aaacccacgt accccatata aagcagaggc ggatggaaga tcagccccgg atcctgtaac
    28201 agcgggttaa gatcgcgccc ttcaatcggg aagttcggca gcgtgcgtga gaacgggtta
    28261 gaggtaaaga gaatgaacag caaaaagccg acgctgacca tccccattat cgccagtaca
    28321 cgggccacaa tatccagcgg aatacgctga ctaaaaatcg ccaccgcaaa ggtccagccg
    28381 ctcatcagca gcacccacag cagaagcgaa ccttcatgcg cgccccaggt agccgccacg
    28441 cgataccaca ccggaagctg ggtattggag ttgctggcaa cataggtgac ggtaaagtcg
    28501 ttgaccacga aagcattgac cagcaccaga aatgcgccag ccacagacat aaacagcagc
    28561 caggcaaaca agcgggaaga cgccatcatg cgcgcatctc cgcgcgccac gccccatagc
    28621 ggatacacgg acagcagcag cgcaatccct aacgccaagc acagcagccc gtttccaatt
    28681 tccggcatca tgatgctggg tccttataaa cactcgccgg gcgacggtgg ttagcttcca
    28741 tcgctttctc aacttctggc ggcgtgtagt tttcgtcgtg tttcgccagc acttctttcg
    28801 cgaggatatg attgcctttt tccagctcgc cctgcaccac aacgccctgc ccttcacgga
    28861 acagatccgg caaaatgcct tcgtaagaga catccactga gccttcagca tcgtaaatgg
    28921 tgaaggtcac tttcagcgaa ttgggatcgc gctgcacgct acccggcatc accatcccgc
    28981 caacgcgcag acgctgaccg acttccggca tctgctgagt ttcacgcttg ccgtagagaa
    29041 tttcccccgg cgtataaaag agatcgatat tcgagcgcag cgcatatagc accagaccga
    29101 tagtcagcgc cagccctgcc aacacggcac aggcaatcca caagcggttt ttacggcgaa
    29161 tattcatgca gcctcctgct gttgcgcagc acgtaaacgc gcctcacgcg cccgctgttg
    29221 cgccacacca cgcagaattg cgcgatgttg catcaccgag tgcacgacca aaaccaccag
    29281 cggaataacg gtcatcacca ccgccagcca gacaaaaaag gcgtaaccgc ccattgcgaa
    29341 aaattcattc caggaagcaa atgcaggggt catttacggc ctcttttcag tatcagttca
    29401 ctcacccacg gacggcgttt ttccatcagc aaaatcaaat tacgcatccg catcagcgtc
    29461 agcgtggcag acaggagcag gaagccaaaa atcgaccagc gcagcggcga acgcatcgcc
    29521 ggatcgatac tttgctgcat ccgcgttgat ccctgatgca gggtgttcca ccactccacg
    29581 gagtaatgaa taatcggcag attcaccacg ccaatcagca ccaggatacc tgccgcacgg
    29641 cccgccagac ggcggtcgtc gaaggcgtgc cacagggcaa tcacacccac atacaaaaac
    29701 agcagcacca gttcagaagt cagacgtgca tcccataccc accaggtgcc ccacatcggt
    29761 tttccccatg cagagccggt aaccagggca ataaaggtaa acacggcacc aatgggggcc
    29821 atcgccgcca ccgccaggtt ggccattttc atctgccaga caaggccaat aaacgccgcc
    29881 accgccattg atgcataaat gcccatcgac cagatcgccg caggcacatg caggtagata
    29941 atgcggtagc tatttccctg ctgataatca gccggagcaa agccgaatcc ccagatccag
    30001 ccgacggtaa gcacgaccac actggcaatt gccagccacg gtataaacca gccacagatt
    30061 tgatacagcc gtggtgggat cgccagttga tgcagtgttt tccacatagt ttcgatacca
    30121 gactcgaaca aaaatcagta atccagcgtt attgaatgct gattcgtaac gctgccgccg
    30181 tcgcaaaagg acttaatgtc gcggtgcctg ccagcaacgc gcctaaaatt gccagatacc
    30241 catcaacggg caaatgcata gaagccgcgt ccatcgcggc ggtggcaaag atgagtaatg
    30301 ggatagtcag cggtaacacc agtatgctga gcagcacacc accgcgctta agtcccactg
    30361 tcagcgccac gcccggtgca ccgagaaagc caagcgtagg cgttcccagc agcagcgtca
    30421 gcgccatcac ttgccagcca taaacatcca ttcccagtag cattgctacc agtggcgaaa
    30481 ggatgagtaa cggcagaccg gttaccatcc agtgcgccat caccttcgcc agcacaacgg
    30541 cgggcaaggg taacggcaac aacatcaatt gttcaagact gccgtcctgc aaatcgtcac
    30601 ggaacagtcg ttccagcgcc agcaaggatg aaagcagcgc agcaacccag ataatgcccg
    30661 gtgcaatacg cgccagcagt tgcggctccg gaccgatact gagcggaaaa agggtaatta
    30721 caatcaggaa gaaccacagc gggttggcga tttcggcgct atggcgaaac gctacgcgca
    30781 gttcaagacg gaaaatacgc cagaacatca tgcggccctc gtttgcgtca gtgaaatgcg
    30841 gcgaatttta ctttcagcaa cgttgagcgg ctggtgggta gtcagaatca caatcccccc
    30901 ctgctccgta tgctgcgcca tacgctgggt cagacgatcg acgccgttaa cgtcaatcgc
    30961 ggtaaaaggc tcgtcgagga tccataatgt ggcacgggtc aaccacagac gcgctaaagc
    31021 gacgcggcgt tgttgcccgg cagaaagttg atttaccgga atatcttcga atccggcaag
    31081 cccggcctgc gccaaggctt ccagacattg tgcggcatcg ccatcgcgat gataaaagtg
    31141 cagattttct aacgccgtca gccgggtttt gatccccggc tgatggccta tccataacag
    31201 gttttgatgg tagctgtcgc gtacctgatg caagggctgc ccttgccaga gaacctcgcc
    31261 agcgtcaggg cgagacaacc ccgtcagcaa acggagaagc gttgtcttcc ccgcgccgtt
    31321 gctaccggtg atttgtaccc actctcctgc gttcagcgta aatgacaagc cactaaataa
    31381 ggttcgttca tcccgctcac aaagtacctc tctggcttca agcataccca cgcaataacc
    31441 ctgttaaaaa cctggctcga cttcacgcat atcgggcagc ttgtgcgcga tccctttatg
    31501 gcaatcaata caggtttgcc catctttcac cgcctggtca tgcatcttcg cggcaaccga
    31561 tttctgggcg gttgtatcca tatactcgaa gttgtgacag ttacggcact cctgcgagtt
    31621 attgtccttc atgcgccgcc actcattctg tgccatcgtc agacgatgag cttcaaattt
    31681 ctgcggcgtg tcaataacgc caaaaatttt accatacagc tctttacttg ctttgagctt
    31741 gcgtatcatc ttcggcacaa actcgtgcgg aacgtgacaa tccggacagg tcgcacggac
    31801 gccgctacgg ttgttgtagt gcacggaatc catgtattcc tgatacaccg tgttgcgcat
    31861 ttcgtggcag ctaatgcaga actcttcggt attggctttt tccatcccgg tgttaaagcc
    31921 cccccagaag acgatgccgc caacaaaacc gatcaacagc agcgtcccca gcgccagatg
    31981 gctgggggta cgccaccatt tccacaggcg cttaatcaga ccaggcttac ggtcagaatt
    32041 tcccataata acctcttatt tcccgtaacc ttttgatggg gtaaaggtat tccccacgat
    32101 tggcgcggta tcggcctgcg gtacgtgaca ttgcagacag aaataacgac gcggagccac
    32161 ttccgcgccc actttgccgt cgctgtccat aaagtgagta ggactgatac gcggcgcgcc
    32221 agtggtgcga tagctttcga caccgtggca ctgcaagcag cgattggtat tggtcgttac
    32281 ctgataacct tcaacgctat gcgggatcat tggcggctga ttcacatagt tcagcggcat
    32341 ccgatcctgc tcttttggca tccgaatggc cccttcctgc gtcccggata cttccggcga
    32401 ctggctaaaa tcgacgccat tagccgccca aaccgcgccg cttaccacca gggccagcat
    32461 cgccgtccat tgacacagcg ctttcttcag gtcatggctt ttcatgattt cgctcccgaa
    32521 ctccatcgtg tagttattgt aaatacatcc tcagaacaga catccacgca gcgaccgcac
    32581 gtcatgcaat cgcggctggt gacctgcacc gggctttgtt catccagcac cggggcacgt
    32641 agcacatgcg gttccgggca aacatgaaaa caatccatac agcggttaca tttctgccga
    32701 tcgctggccg caacggtaat cacacctttg ctacccagca cgccatacag cgcgccaacc
    32761 ggacaaatgt gcccgcacca gccgtgttca acgaccagta gatcaaataa aaacagcgcg
    32821 agaataagca gcgcgccgct gccgaagccc attaccaggc tacgccccat cagagaaacg
    32881 gggttgatcc attcccaaat gagcgtgcca gttaaggctg atcccaccag aatgaccacc
    32941 aacagcacgt agcgaatgtg gcgagggatc gtcgcagact gattgaggtc aaagcgcctg
    33001 cgtaaccagt tcgctaagtc ggtaatcggg ttcagcgggc agacccagct gcaaaataat
    33061 cgctttcccg ccaaggcgta gagcacggtg ataatcaccg caccggtcaa cgccaccgtg
    33121 gcaggcagat gaccactggc aagactttgc agcgtcatca gcggatcggt cagcggaacg
    33181 gtgtcgaata acaggctact gctgtagttg ccgtgcaaga tccacacacc aaaccatgga
    33241 ccgctcaaaa acatccccag cacgaagaac tgacaaaggc gacgtaacac cagccaacgg
    33301 tgactgcgcc accagccttt tttctccagc gcctcgcgcc cggcgtcacg tttacgattt
    33361 gccattgttc ccctccagcc agccgaagcg gtaatggtga cctaactccc ctttcgccag
    33421 tgacagcggt aacaccttga ttgccggttg ttccagcacg catacttttt cgcacttacc
    33481 gcaaccggta caggcgtcgc tgtgaaccgt cggcagaaag cgggcgtgct taccggtacg
    33541 cgtgttgcgc tccagctcca aggtgatggc ctcatcaatt ttcgggcatt cgcgataaca
    33601 aacatcgcag cgcagcccct gaaagttgag acagttttcc tggtccacca gtaccgccag
    33661 ccccatccgc gcgtcgtcga tcgattcaat ctcacgatcc agcgcaccgc ttgggcacac
    33721 tttggcgcac ggaatgtcct cacacatttc gcaaggaata tcccgtgcga caaaatatgg
    33781 cgtgcccgcc gacagaccag aggccagcgt cgccagtttt aaggtgtcgt aagggcaagc
    33841 ctgaacacac tgaccgcaac gcacacaggc actggcaaag gcgttctcgt ttatggctcc
    33901 gggcgggcgc aaccgcacgc cagatgcgcg tgcggtttgc tgttgtaacc ccagcgccac
    33961 acccacggca gccagcccgc ctgctgtgcg aacgacatcg cgcagaaagc ggcggcgacc
    34021 attttgaggt ttcgctgacc gggacataat ggcttacacc ttctccagtt tgaccgcgca
    34081 cttcttgaag tccgtctctt tcgagagcgg atcggtcgca tccagcgtca gtttgttaac
    34141 cagctgtgcg gcgtcgaaga acggcatgta caccaggccc tgtggcggac ggttacgacc
    34201 gcgcgtttca acaatcgaga tcacttcgcc acggcgagaa accactttca ctttgtcgcc
    34261 acggcgcaga tcgcgcgctt tcgcatccag cgggtgaata aacaagaccg cttccgggaa
    34321 ggcgcggtgc agttccggca cacggcgagt catactgccg gtgtgccagt gctccagaac
    34381 gcgtccggta gagagccaca ggtcgtactc ttcatccggt gcttccgccg ccggttcgaa
    34441 cggcagtgcg aagatcaccg ctttgccatc cggtttaccg tagaacttat agccttcgcc
    34501 cgctttcacg tacgggtcgt taccttcgct gtaacgccac tgtgtttctt taccattcac
    34561 caccggccag cgcagaccgc gcgctttgtg gtagtcatcg aacggtgcca gatcgtgacc
    34621 gtgaccgcga ccaaaccatg cgtactcttc gaacagccct ttttgcagat agaagcccag
    34681 ctcgcgggat tcatcgttca gctgatcttc cgccagttcg gataccggga atttgctcac
    34741 ttcgggggtg gcatacagaa cttcgtacag cgttttgcca cgcagttccg gtttcttcgc
    34801 cagcagctct tccggccata cttcttcagt tttgaagcgg cgggagaact ggactaactg
    34861 ccagagatcc gatttcgctt cgcctggcgc ctgtacctgc tgacgccaga actgagtacg
    34921 gcgttcggcg ttaccgtaag cgccctcttt ctctacccac attgcggtcg gcaggatcaa
    34981 gtcggcggcc agcgcactga ctgtcggata cggatcggag acgatgatga agttgcgcgg
    35041 atcgcgccag cccggcatac gctcttcatt gatgttcggc ccggcctgca tgttgttggt
    35101 acacatggtc cagtaaacat tcagcttgcc gtctttcagc gcacggtctt gtgccaccgc
    35161 atgaagaccg attttcgccg gaatggtgcc gctcgggata ttccacttct tctcacagat
    35221 atcacgatgt ttctcgttag ttaccaccat gtccgcaggc agacggtgag caaaggtgcc
    35281 cacttcacgc gcagtaccac acgcagaagg ttgcccggtc agggagaacg gaccgcaacc
    35341 cggctgggaa attttgccgg tcagcaggtg caggttgtag accaggttgt tggcccacac
    35401 accacgggta tgctggttga agcccatcgt ccagtaggag atgactttct tgttcggatc
    35461 ggcatacagc tgcgccagtt gttctaactg gtctttcggc acgccggtca tttcagcggt
    35521 tttttccagc gtatactcag caacgaaggc tttgtaatct tcaaagctca tcggttcgga
    35581 ggcgtcagaa cccggattct tcgctgcttt ttccagcgga tgggtcggac gtaaaccgta
    35641 gccgatgtcc gtcgcccctt tgcgcaggtt aacgtgcttg ctgaagaagt cctgatttat
    35701 cgcattgttt tgaatgatat agttggcgat gtagttcagg atcaccaggt cagattgcgg
    35761 cgtaaagatg atgccgttat ccgccagctc gaagctacga tgctggtagg tagaaagcac
    35821 cgccacggtg acgttctggt tagagagacg acggttagtg atgcgtgacc agaggatcgg
    35881 gtgcatctcc gccatgttag cgccccacag cacaaacgcg tcagcctgct cgatgtcgtc
    35941 atagcagccc atcggctcat ccataccaaa ggtacgcata aagccaacta ctgccgacgc
    36001 catacagtga cgtgcgttcg ggtcgatgtt gttcgaacgg aagcccgctt tgaacagctt
    36061 ggacgcggca taaccttccc agatagtcca ctgaccagaa ccgaacatac cgatcgattc
    36121 cggccctttt tctttcaggg cggttttgaa tttctcttcc atcacatcga aggcctgatc
    36181 ccaggtgatt ggggtaaatt cgccttcttt gtcatattta ccgtttttca tacgcagcag
    36241 cggctgcgtc aaacggtctt taccgtacat gatcttgggc aggaaatagc ccttaatgca
    36301 gttcaggcca cggttaaccg gtgcgtccgg gtcgccctga caggccacca cacgcccctg
    36361 ctgcgttccg accagaacgc cgcaaccagt accgcagaaa cggcacggcg ctttatccca
    36421 tttaatggct tcctgctgac caacaacggc gcgggcaacg cccggcacgc tgagaccggc
    36481 agccgccgca gcggccgcaa cggcgttagc tttcataaag ctacgacgac tgagtttcat
    36541 ggtgtttcct caccttgctc ttcctgctgg tgataaacca gcgacaccgc cagcacgccc
    36601 tctacgttgc gtactgactc aatggtttgg atcagcgttt cgctgtcttc tgcttccacc
    36661 accacaatca actgaccgct cggcgcgtcg ctgacagcaa cttcacagcc gggaaaggcg
    36721 ttcagttggg tgctgatgtc tgaaattcgt tcgcttttgg cctgcacgac caggctgcaa
    36781 acttgccagt tagtgtgcat ggagatactc cgcagttatg gctgataccg gacagctggc
    36841 tgcacatgct ccacatccgt tgcaaagttg gctattaagt tgcgcctggt agatcccgga
    36901 gagcgtcggg cgaaagataa ttgccattgg ctcacagcta tcctgacagc ggcggcattc
    36961 aactgactga tacgccaggc acgcgtcccc gatggtgaac tgcaaatccc aggccctggt
    37021 gtggcgcgga gaaaatagcg attcggggca agcctgcgcg caggcgtaac agaagctgca
    37081 ctcgttattt ttgaaattaa cgctcggata gccacccgtg ccgcgttgca gaatgttgtt
    37141 ttcacaagca ttaatacagg cgtcgcagcg ggtgcaatgg gtcagaaaat gagattcatc
    37201 accggaccag ggcgggcgga taccgttact ggctttgcgc cagcgaccag tgagtatgcc
    37261 ccgacgggat gcatcaatct tcacattgac cttccatcat taacgcgctc tgaaaattga
    37321 gagcgaccaa atagaccgca taattaataa gccattttta tagccgctaa gatattaaag
    37381 gatgtgtcaa agatgcatac cccgatcggg gtaaaacctg cagcaggatc aaaagagtgg
    37441 cgggaagcgt ggcaaaaacg ggcttttgct cacatttcaa atgtttataa acatatttat
    37501 atagcgatta attcaccaga gatatttctg ctggtttgct ccctcatcag aatttaacac
    37561 taaaagagca ggtaaaattg tctgaatgtt ctttaagtta ttcataaagc aaattaataa
    37621 atctgatgaa tatgttaacc ttcagcgaca tcatcggtga aaacctataa atgaagaagg
    37681 aaagcaaaaa aatgaagacc attctacctg cagtattgtt tgccgctttc gctaccactt
    37741 ccgcctgggc ggcagaaagc gtccagccac tggaaaaaat cgcgccttat ccacaagctg
    37801 aaacaggcat gaagcgtcag gtgattcagt taaccccgca agaagatgaa tctaccctga
    37861 aagtagaact gttaatcggt cagacgctgg aagtcgattg caatttgcat cgtctcggcg
    37921 ggaagctgga aagcaaaacg ctggaaggct ggggctatga ttattatgtc tttgataaag
    37981 ttagctcgcc ggtttccacg atgatggcct gcccggatgg caagaaagag aagaaatttg
    38041 tcaccgcgta tctgggcgat gctggaatgc tgcgttacaa cagcaagctg ccgatcgtgg
    38101 tgtatacgcc agacaatgta gatgtgaaat accgcgtctg gaaggcggaa gagaaaattg
    38161 acaacgcgga agttcgctaa actgttgtga agtacggcac cccgttggtt ggataaggcg
    38221 gttacgccgc atccaacatc taacgcccga gccggttgcc tgatgcgacg ctggcgcgtc
    38281 ttatcagtat acgccacatc cggcatacca tgccggatgt ggcacatcat tacaacgcaa
    38341 tatccgccac ttctttttga acgggttgcg gtttcaactg cggtttcggc gtactatctg
    38401 ctgcttgcgg tttgtcgtat ttcagcccca gcacttcgct ggtgtactgc aactcgcgtt
    38461 ctgttgccgc tacatcaccg ttcagcttgc gtccataaga cggaacgatc gctttcaacg
    38521 tagcctgcca ttgcgggctg gaaacgcgat cgccaaatac tttttccagc agattcaaca
    38581 taatcggcgc ggcggttgac gcccctggcg atgcccccag cagcgccgca atggtccctt
    38641 gctggtcact gaccacttcg gtacccagac gcagtacgcc acctttctcg gcatcacgct
    38701 tgataatctg cacacgctgc cccgcttgcc acaaacgcca gtcctctttt ttcgcttgcg
    38761 gatagtactc tttcaacgct tcaaaacgat cctcttcact caacatcacc tgactcacca
    38821 gatatttcac cagctcgaaa ttatccagcc cgacgtgcat catcggcatc acgttagagg
    38881 tggtggtgga actcatcaga tcccacaatg aaccgttttt gaggaatttg gttgagaagg
    38941 tggcaaatgg cccaaacagc actacgcgtt taccgtccag aacacgggta tcgatatgcg
    39001 gaaccgacat cggtggtgcg ccaacggatg ctttaccgta aactttcgcc agatggcgat
    39061 taaccacgtc tggattttcc gaaacaagga actgtccgcc caccgggaaa ccggcgtagt
    39121 ctttcgcttc cggaatcccc gattcctgta acagcttcag cgccgcaccg cccgcgccga
    39181 taaagacaaa tttggtgcgg atgttctgtg cagtgccgtt tttcagatcg gcaacggtaa
    39241 cggtccaggt gttatcgtca ttacgcttta gggcgcggac ttcgctgctg agttgcagcg
    39301 agaagttaga tttcttctgt aaggaagcaa ttaactggcg ggtgatctca ccatagttca
    39361 catcagtgcc aatttctgtt gcggatcgcg cccttccatc actaacggtg cccactcttt
    39421 gatctgcgcg tgatcttcag agtaacgcat accgcgaaac agcgagcttt gttgcaacgc
    39481 ggcgtaacgg gcgcgcagga aattaacgtt atcctcgccc cagacaaagc tcatatgcgg
    39541 aacgatattg ataaatgaac gcggagtacg cagcacgccg cgctcgacct ggtgcgccca
    39601 gaactggcgg gaaatctgaa atgcttcgtt aatggcgact gctttttcaa tactgatgct
    39661 gccatcggcg ttttgcgggg tgtagttcag ttccatcagt gcagaatgcc cggttccggc
    39721 gttgttccag ccgttcgaac tctcctgcgc gcccccctcc aggcgctcca ccatggtcat
    39781 cgaccattca ggctccagct cgcgtaaata ggtccccaac gtggcgctca taatgccgcc
    39841 gccaatcaac agtacatcag tttcctgctc ctcggacgct ttcgcttttg ccgccatcga
    39901 aacggcatta agccccacgg ccatcgagaa gagcatggca gtcacttttt tcatcttgtt
    39961 aatgccttac ttttagtcgc tttattgcag gtgagatttg cgcggcatca acggtaacac
    40021 ttaagtaaca acatttaaat aatgtttata aattatattc taattttaga aacattataa
    40081 tttcgttgat taattatagg gttattagaa atgaagggat ttttagcata ttgacctgac
    40141 ggcagcagtc cgccaggtca gcgtcgtaat attatgccgt ccgggcaacg gcatcacgcg
    40201 aagcggcatc gcgctcttcg cccgtcagct cgctaagttg cccattgcgc atttccagca
    40261 ggcggtcggc gtggataaag taatgatcat catgactgat agcgaaaata gttttaccca
    40321 tctcctgcat cagcggcagc aacacctgat aaaactcacg acggaagtgt ggatcctgat
    40381 ccgccgccca ttcatccagc aggataatat cgcgttcttc tgccagcgcc agcaacagcg
    40441 gcacgcgttt tttctgccct tttgataact tcaggttaac aatacgcccg ttgcttaact
    40501 caagcttatg agccattttc agctgcgcca gccacttctc aaccagttgc gggttagcgg
    40561 gttgcccctc cggccccagc agttgatcaa acagccagac atcggtaaac actgccgaaa
    40621 acagtttgcg ataatcttcc ggttgttcgc cgctgacagg tttgccatcc agcaagattt
    40681 cgccgctttg tggctgatac aagcccgtca acaacatcgc cagcgtcgat tttccgctac
    40741 cgttgccgcc aatcagaaac agcagctcgc cacgtttgat ggtgagatta atcggaccaa
    40801 cggaaaacgc gttatcctga taagcaaacg tcacgttacg cagctccagc gtttgccagt
    40861 tgggaaacgc atgcgggcgc ggaaactcag ctttgaaagg cgcgagcgcg aatttgttca
    40921 gcttgttaaa cgccacctgc gccgtcagca gcgtcggcaa tgcgccaacc gccgaaagca
    40981 gcggcgtacg caggaataac agcgtcaacg aataggtcgc agcaacgttg gtatcagccc
    41041 aaccgagact gttcgccatc cagaacacca ggccgatagc gcccagcatc atgatgtttg
    41101 accagttcac ggcactaaga tggaaggtgt ctgcgcgaat aatatgatgg cgatactctt
    41161 gcgcatcggg gatgtagagg ttgttaaaca catactcggc gcgttcccgg ttcagagtca
    41221 gctctttgcg cccttcaagt acagtttgaa aatccgtgta cagcttgtct tcggtttcac
    41281 gtagggtcgc catatgtttg tacacccgcg ccaccagcac aaaaccgccc cagatggtga
    41341 tcgccatcca gatagccgtt accagcaaca ttttgcccga cagcatccac agatacgccg
    41401 atgaaccgat agtgagaatg atcccctgcc ccagttccgg cagacgcaca aaagcaatgg
    41461 tgatattgcg cacatcgctg gttaatcccg ccagcaacga ggcgctaccg agttgttcaa
    41521 tgcgctcgac gtgggtatcc agaatccgct tgataaattc actacgcagt cggtaaacga
    41581 agtgatgccc caaagtggtg agcgccagtt gcgatccgag agtgactgcc atcaacagca
    41641 gcaataatcc cagaaactcc ggtaacacca gcagactggt atccgccgtt tcgataaggc
    41701 gctgattgat aaaagcaatt aagccaatgc ctaatgccgc actggcgagg cttagcgcca
    41761 tcacactgat aaatggccag cgatactgcc gccagacaag tacaagaagt tccatgcaga
    41821 aaacccggac aatgaattac agcccgcagt ttaaacatct tcgcgcgcac agcaataata
    41881 attcttattt ttattctttt ttacctgcct gacggaatgt caggttgtag cggcagtcgg
    41941 tggtgagtgg atgaaacccc gctttcaacg gttgaatacc gtgataaaac agccgcgatt
    42001 caccgcccca ggccaccaaa ttgaaaaatc gcgggtaagc ccagagaaac agaaacaatt
    42061 ggcgcgcgca gatccggttc gtctttatcc tgatgcagcg acagtttcgc gccaggagcg
    42121 tagcggttga taagacaagc atctggctgg aaatctggat agcccgccgc cgtagccgca
    42181 cgttgacata aatcatgaaa gctttgtggc atggcgggcc acggtttatt tgtttgcgga
    42241 tcaatgggcg aatagagata accttgccga tgggtcgtcc agcccagatg cccacagttg
    42301 gtcatcgcca ccgacatggt atatccgcct ggagcgacca tctggcgaaa cggcgactgg
    42361 ctggcaacgt cattaatatc gcggattagt tgctccgcag cattaaaagc aaaacgccgt
    42421 aaaattaccg caccagccgc cagtggctct tgccacggtt cagcatcggc aaacagatcc
    42481 aacattacct ctcctcattt tcagcttcgc ggcgcagcag ttgcgcttta cgcgacacgc
    42541 cccagcggta accggaaagt gtgccatcac cacggaccac tcgatgacag ggtattacga
    42601 tagccagctt gttggcggca caggcgctgg caacggcccg taccgctttc ggtttgccga
    42661 tggcgttagc cagttgctga taactgacgg tttcaccaca aggtatcgtg cgcagtgcct
    42721 gccagacttg ttgctgaaaa gcagtgccgc gaatatccag cggtaacgtc agcggcgtat
    42781 cgcgttgatt gaggctggcg atcacttcac gcacatgttg ctgaaacgtc agatcggcag
    42841 gcgcgttgtc ggcagcggga aacatctgct gcaactcgct gattaatgtc gcgtcatcat
    42901 cgcccagcaa tatcgcgcaa atcccccgct cgctttctgc caccaggcaa cgacccagct
    42961 cacaatcagc cagcgcgtaa cgcaccgcca gattttcgcc accgtgacgg aattgtttag
    43021 ccgtcatgcc cagcgtttca tcggctttgc ggtagtaact gctgctgtcg gggaatccgg
    43081 cgttaagaat agacgtcgtc acgctctccc ctttcgccag cgattcgcgc aaacggcgag
    43141 cgcgccaggc ctgttgccag gctttaggcg tcattccggt agtagcttta aacaaccgat
    43201 gtagatgaaa tggactcatc gccacctggt cggctaaggc ttccagcgtt acaggcgttt
    43261 cctgttccag cagtcgacac gcgtgggtga ttttatccaa ccgatgttgc cggggattgg
    43321 ctttgtctgg ctgacaacgt ttgcaggggc gaaatccggc ggcgagtgcc tcgctggcat
    43381 ttgcgtagaa ggagacattt tcccgcaagg catgtctggc gcggcaagac ggacggcaaa
    43441 agatacccgt ggtgcgcacg gcgaaaacga attcgccgtc ggcattcggg tcgcgggcta
    43501 agacagattg ccagcgttga tcgtcagtta agcatgtggc gtttttcata atcagctccc
    43561 tggttaagga tagcctttag tctgcccggt caccatcacg caaaaaccaa caatcttgcg
    43621 ctttaatttt tttcgctgac aaggaagctt ttaaactgtg gtgacatcca ggttttaaag
    43681 ctatcgcctt ctttggtgat catataaacc gccagccctt cccggcgaac aacctctttg
    43741 gctttctccg gcccgagtac catcaagcca gtatcccagg catcggcttc cagcgccgtc
    43801 ggagcaatca ccgtcacgga taccagattg tgttcgatgg gacgcccggt ttgcggatcg
    43861 ataacatggg aaagacgttt gccgtccagt tcgtaatagt tacggtagct gccagaggtg
    43921 ctaatcccat gaccgttgat atccaccaca gcctgaaccg cgttttcttt atcggttggt
    43981 ttttgaatcg ctacccgcca cggctggcct tcaccgttca taccacggct gttcagcgcg
    44041 ccgcccaccg acaccagata gcgggaaatc ccttcctgct ccatcaagcg tgccaggtga
    44101 tccgccgcat aaccttcacc gacggtggag agatcgacat ataaatccgg caggtctttt
    44161 tgcagatatt gctgatgcga ctgattgatg accgtcaggt gctgtaagcc ggttttggct
    44221 ttcatcgcat cgatctgttc ctggctcgga atttgaaccg gctgttgttc cgggccaaag
    44281 ccccacagat tcaccagcgg cccgacggta atatccatcg caccatcggt ccttgcccca
    44341 atgcgcagcg aggtggtgac gatatcggcc atcgcttcac ttaccggcca cggcgacaaa
    44401 ctttgcgagt cgttaaagcg catcagcgcg gaatcttttt tataggtcga aagcagctga
    44461 tcgtcggcgt ccagctgggt ctgaatcttt tctttaagtt cggcactgcg tttggcatca
    44521 atgcccggga tgctggcacg ccagaaggta cccatggttt tgccttcaag aacggtaact
    44581 tcagtagcgt gggttttggc gggttgtggt ttttgatcgc aaccaacaaa gaagagcgcg
    44641 gcaaccagca gtgccacgcg ggtaaagctt atttccatac gtgattatcc tcatgcgaac
    44701 ggtcgcaaga gtacaccaaa aaactgtgtt tgtacgctga aaacaatgaa aaaagggccc
    44761 gcaggccctt tgttcgatat ccatcgagat tagaactggt aaaccagacc cagagctacg
    44821 atgttatcag tgttgatgcc agcgtcacga gtgaactggt tgtcgtccag caggttgatt
    44881 ttgtagtcaa cgtaggtgga catgtttttg ttgaagtagt aggtcgcacc aacatcaaca
    44941 tatttcagga tatcttcgtc gtcgtagtta cgaccattga tgacacccag gtttttacct
    45001 ttagactgca ggtaagctag ggacggacgc agaccgaagt cgaactggta ctgagcaaca
    45061 gcttcgaagt tctgtgcttt gttcgcccaa cccaagttgc ctacgcgagt tgcgttgtag
    45121 gtctgggtgt actgagcagc taggtagatg ttgttcgcgt cgtatttcag accaccggtg
    45181 taggtttcag cgcggtcgcc attaccgatg taacgctcat cataacggtt cagaccgaag
    45241 ttctggtcat cagtacgttt ggagctggaa actgcagcac cgataccgaa gccttcgtaa
    45301 tcataagtaa tagaaccgcc aacgccgtca ccgttctgac gcagagcttc acgaccgttg
    45361 ttggtcatgc cttcaccttc cgggctaccg tttttgccct gatactgaac agcgaagttc
    45421 agaccgtcaa ccagaccaaa gaagtcggtg ctacggtagg ttgcgaagcc gttaccacgc
    45481 tgctgcatga agttgtcaga accgtaagtg tcaccaccga attctggcag tacgtcggtc
    45541 caggaagtta cgtcgtaaac tacgccgtag ttacgaccgt agtcgaaaga acctacatcc
    45601 tggaatttca gacctgcgaa tgccacacgg gtccaggagt tgttttcgtt ttcagcgctg
    45661 ttgccctgga tctgatattc ccactggccg taaccggtca gctggtcagt aacctgagtt
    45721 tcacctttga agccaagacg catgtaggtc tggtcgccat ctacagactt gtcgtcagag
    45781 aaatagtgca ggccgtctac tttaccgtac agatctaatt tgttgccgtc tttgttgtaa
    45841 acttcagcag cgtttgctgc tcctgctacc agcagagctg ggaccaggag ggacagtact
    45901 ttaactttca tgttattaac cctctgttat atgcctttat ttgctttttt atgccactgc
    45961 atactgatta accctcatta atcagtcggc aagtccattc tccccaaaaa tgcagaataa
    46021 tccaacacga atatgatact aaaactttta agatgtttca tttatcgcta tagatgtttc
    46081 aaaatgtaaa tacaagggaa ctttttaaga ttattgcgga atggcgaaat aagcacctaa
    46141 catcaagcaa taataattca aggttaaaat caataactta ttcttaagta tttgacagca
    46201 ctgaatgtca aaacaaaacc ttcactcgca actagaataa ctcccgctat catcattaac
    46261 tttatttatt accgtcattc atttctgaat gtctgtttac ccctatttca accggatgcc
    46321 tcgcattcgg tttttttacc cttctttaca cacttttcat tattctgtgc taccacagaa
    46381 aaactataac gcttgttaac tatttcacaa ataattaaca tcagcataat ttccagcaat
    46441 ctttgtttat ttacaattaa tttcgttggg ctttttgtag gttatttgta cagcaaaatg
    46501 gcgcttgtac atctatttcc ccccatacag gatgataaat atcacgggag aatagagaat
    46561 catcaatcag gtaagagtct ggaatttcac actgtaccct ttatactgcc ctatcacttc
    46621 gcgaagtttt aacaggtcat aaacacgaat gcgtcagaaa gagacaacgg ccacgacccg
    46681 cttttcactc ctaccgggga gcattacccg cttcttttta ctgttgatca ttgtgttact
    46741 ggtgacgatg ggggtaatgg tacaaagtgc cgttaacgcc tggctgaaag ataaaagtta
    46801 ccagattgtc gacattaccc acgcaatcca aaagcgcgtc gatacctggc gttacgtgac
    46861 caggcagatc tacgacaaca ttgccgcgac gacctccccc tcctccggcg aaggtttaca
    46921 agagacgcgc ctgaaacagg atgtctacta tctggagaaa ccacgccgca aaacggaagc
    46981 gttaatcttt ggctctcacg acaactcaac gcttgagatg actcagcgga tgtccactta
    47041 tctggataca ttgtggggtg cagaaaatgt accgtggtcg atgtattacc tgaatggtca
    47101 ggataacagt ctggtgctga tctcaaccct accccttaaa gatctcacct ccggatttaa
    47161 agaatcgacc gtcagtgaca ttgttgattc acgtcgtgca gaaatgttgc aacaggccaa
    47221 cgccctcgat gaacgcgaaa gtttttctaa catgcgccgc ctggcctggc agaacggtca
    47281 ttactttacc ttgcgtacca cgttcaacca gccgggacat ctggcaacgg tcgtggcttt
    47341 tgatctgccg attaatgatt tgatcccacc gggtatgccg ctggacagtt tccgccttga
    47401 gccagacgcg acggcaacgg gaaacaatga taatgagaaa gaagggacgg atagcgtcag
    47461 tatccacttt aacagtacga agattgaaat ctcctcggca ctcaactcta ccgatatgcg
    47521 cctggtctgg caggttcctt atggcacctt attgctggat acgttgcaaa acattctgct
    47581 gccactgctg ctgaacatcg gtttgctggc gctggcgtta tttggctata ccacattccg
    47641 ccatttctcc agccgcagta cagaaagcgt ccccagcacg gcggtcaata acgaattgcg
    47701 cattttacgg gcaattaatg aagagatagt ctcactgttg ccgctcggcc tgctggttca
    47761 cgatcaggaa tcgaaccgca ctgtcataag taacaaaatt gccgatcatt tgctgccgca
    47821 tctgaatctg caaaacatca ccaccatggc ggaacagcat caggggatta ttcaggcgac
    47881 gatcaataac gagctgtatg agatccgcat gttccgcagc caggtcgcgc cgcgcacaca
    47941 aattttcatt attcgcgatc aggatcgcga agtgctggta aacaagaaac tcaagcaggc
    48001 gcagcgtctg tatgagaaaa accagcaggg gcggatgacc tttatgaaaa acattggcga
    48061 tgcgctgaaa gaacccgcac agtccctggc ggagagtgcg gctaaactca acgccccgga
    48121 aagcaaacaa ctggcgaatc aggcagatgt gctggtgcgg ctggtcgaag aaatacagtt
    48181 agcgaacatg cttgcggatg atagctggaa aagtgagacg gtgctgttct ccgtgcagga
    48241 tttaattgat gaagttgtgc cttcagtgtt gcctgccatc aagcgtaaag gtctgcaact
    48301 gctgattaac aatcatctga aagcacacga tatgcgccgc ggcgatcgcg atgccttacg
    48361 acgtattttg ctgctactga tgcaatatgc cgtgacctca acgcaattgg gaaaaatcac
    48421 ccttgaggtt gatcaggatg agtcctctga agaccgcctg acattccgca ttctggacac
    48481 gggagaaggc gtaagtattc atgaaatgga taatttgcac ttcccgttta tcaaccagac
    48541 ccaaaacgat cgttatggaa aggcggaccc gctggcattc tggctgagcg atcaactggc
    48601 acgtaaactg ggcggtcatt taaacatcaa aacgcgggat gggcttggta cacgctactc
    48661 tgtgcatatc aaaatgctcg ccgctgaccc ggaagttgaa gaggaagaag agcgtttact
    48721 ggatgatgtc tgcgtaatgg tggatgttac ttcggcagaa attcggaata ttgtcactcg
    48781 cctgttagaa aattggggtg caacctgtat cacacccgat gaaagattaa ttagtcaaga
    48841 ttatgatatc tttttaacgg ataatccgtc taatcttact gcctctggct tgcttttaag
    48901 cgatgatgag tctggcgtac gagaaattgg gcctggtcaa ttgtgcgtca acttcaatat
    48961 gagcaacgct atgcaggaag cggtcttaca attaattgaa gtgcaactgg cgcaggaaga
    49021 ggtgacagaa tcgcctctgg gcggagatga aaatgcgcaa ctccatgcca gcggctatta
    49081 tgcgctcttt gtagacacag taccggatga tgttaagagg ctgtatactg aagcagcaac
    49141 cagtgacttt gctgcgttag cccaaacggc tcatcgtctt aaaggcgtat ttgccatgct
    49201 aaatctggta cccggcaagc agttatgtga aacgctggaa catctgattc gtgagaagga
    49261 tgttccaaga atagaaaaat acatcagcga cattgacagt tatgtcaaga gcttgctgta
    49321 gcaaggtagc ctattacatg aacaatatga acgtaattat tgccgatgac catccgatag
    49381 tcttgttcgg tattcgcaaa tcacttgagc aaattgagtg ggtgaatgtt gtcggcgaat
    49441 ttgaagactc tacagcactg atcaacaacc tgccgaaact ggatgcgcat gtgttgatta
    49501 ccgatctctc catgcctggc gataagtacg gcgatggcat taccttaatc aagtacatca
    49561 agcgccattt cccaagcctg tcgatcattg ttctgactat gaacaacaac ccggcgattc
    49621 ttagtgcggt attggatctg gatatcgaag ggatcgtgct gaaacaaggt gcaccgaccg
    49681 atctgccgaa agctctcgcc gcgctgcaga aagggaagaa atttaccccg gaaagcgttt
    49741 ctcgcctgtt ggaaaaaatc agtgctggtg gttacggtga caagcgtctc tcgccaaaag
    49801 agagtgaagt tctgcgcctg tttgcggaag gcctcctagt gaccgagatc gctaaaaagc
    49861 tgaaccgcag tattaaaacc atcagtagcc agaagaaatc tgcgatgatg aagctgggcg
    49921 tcgagaacga tatcgccctg ctgaattatc tctcttctgt gaccttaagt ccggcagata
    49981 aagactaatc atctgtaggc cagataagac gcgttagtgt cttatctggc atttgcaccg
    50041 attgccggat gcggcgtaaa cgccttatcc ggcctacgat tcccattatt tcaacagatt
    50101 acattaaagt aggccagata agacgcgtca gtgtcttatc tggcatttgc actgaatgcc
    50161 ggatgcggcg taaacgcctt atccgtccta cgaatcccgc gatttcctga ccctctcggc
    50221 atataccgtc agcgtctgtt ttatcacatc cagcgttacc ggcttcgaga ggcagctgtc
    50281 cataccggac tccagacacc gctgcttctc ttcagccaac gcattagcag ttactccgat
    50341 taccggcaac gtcagtccca actgacgaat gcgttgcgtc aagcggtaac catccatatt
    50401 tggcatgttg acgtcgctaa gcacgatatc aatatgattc ttactaagta cattaagcgc
    50461 atcgacgcca tcattcgcgg ttttacattg atagcccaac gatcccaact gatctgccag
    50521 cagacgtcgg ttaatcggat gatcatccac gaccagaatc atcatatcgt cgttatcgct
    50581 gaccgctttg tccgtcgacg gcagagcgtt agcaggatcg tcgctctcca tctcgatcaa
    50641 ataaatacgc gccaacaatg ccggtagctc atgcggagca gccacactgt gtacccactc
    50701 ccctggcgct ttctccagcg gaataccaat atggcgacga cagaaggtca ctaccgctct
    50761 gccctgccat tttttactca ctacctcgtc agtgattaac acatcttcgg gagtcggttc
    50821 ctgcccttcg tatgttgtaa cgacgatgcc gctgcgctgc aaactggttt ccaggaactg
    50881 acagagcgac gcattgcgga ccgccagcca gcagcgttta ccactcaacc cttccacgcc
    50941 ttttttctgc gggtactgag cgccgtacaa cggaatacgc acggtaaact ggctgcccat
    51001 tcccggttct gaatctaccg agatatcgcc gtccatcatg ctgatcagtt tttcacaaat
    51061 cgccagaccc agaccggtcc cctggaagtt tcgctgtacg cccgttccaa cctggaagaa
    51121 gggatcaaac aagcgcacca cttctttcgc cggaatcccc acgccggtat cgcgaacacg
    51181 gatagagaga taatcgccat cagcgcgaac atgcaaaact atacagccgg tatcggtgaa
    51241 ttttatggcg ttactcaaca ggttggagat gacctgctgt aaacgcatcg ggtcgccatt
    51301 taaggccact ggcacatccg gttcaataaa gcagtacaag cctaactgct tgcgtaccac
    51361 cagcggtaaa tagttggcgg tgatgtggtt catcacttca cgcggtgaaa actcacgcgg
    51421 ttcgatcttc aactgttccg attcaatctt cgagaaatcg agaatatcgc tgataatttt
    51481 caacaacagg ctggaagagt tgttcattgc cgtcaccaga cgatcgacgc ctttcggtaa
    51541 ctctttggtt tgcaacagat ccaggttacc gataatgcca tacagcggcg ttcgcagctc
    51601 atgactgacg gtggcaagga acatcgattt tgactggctc gcctgttccg ctgcttgtgc
    51661 catctcctgc aacgactctt ccatcttcac gcgcgaagaa acatccacca gcacacaaat
    51721 ggccacgttt tcattacgat agcgcgaatg gacgaagctg atttgcagat tggtattgtt
    51781 gctggtcagg acatcaacaa aattgacctg ctgcccacag ataatttgcg tcagtcgttg
    51841 gcggtcctca tgcgtaagca tattgagata ggtatgcgcc agttcgttac ttaaaatatt
    51901 gacgccatca gcggtacgca aaatgcagat acccactggc gcggaggcga caatcttgcg
    51961 attgaactgc tcatgttctt ccagtcgcag ggcgtcgctt tccgccggaa tgaaaatacg
    52021 tcgctcgtac atccgcgcga gagtaaacaa tgcagctccg gcaagcacat tcagcaaaat
    52081 tgcgttaagg atcaacatgc gaatgcgttc cagcacctta tcaaccggca ccgaatacac
    52141 gatgcttagc gatgagggtg gcagattttt cttcagcacc agctcccgga acccttccgt
    52201 atagccaaac caggagcgtt cctgcatcca gcgaggatcg cccttaattt tactttctgg
    52261 tccggtaagc gaaatcaggg tatgaccatt ttcatcaaga atggtaaccc ccatcggcaa
    52321 cgtacccggt aagaaaaagt tctccatccg gatggtctgc tcgacaccca aaagcgcctg
    52381 caaccggttc gccagataaa ctggcgtcaa cgcgtaaaaa tacccgacgc cagggcgcgg
    52441 accttcgctg atccagtaga ggttactgcc gctatcatct tgtggtgcat ttcgatattt
    52501 attgatgcgt tcatgcaacg ctttcaacgc ggtatcgcgt tccactggca tatcacgcag
    52561 accgaaattg gccatgcaga ggttatcact gccgattaaa aatacccggt tgagatcgta
    52621 agccgcagaa aaattatcgc gccagtagcg cataaaccac gccaatgact ccagagaacc
    52681 tcgccaggtg ttactcattg cggaacaatc ggagtcggca aacagcggtt caaacgcagg
    52741 cacatccgcc tgcgtttctc gtccacgcgg ggaaagcaca ccgttttctg ccgataagcg
    52801 attttcggcg atgtacttaa gctctttcat cacatcagag gtgcgttgaa taaagcgctg
    52861 agcctgatcg gaactcagat taaattcctg acgaatttcc gattctcgct gatgtaacgc
    52921 attaacgatg taaaaaacgg atgaaaaagc aatcaacagc cagagcacta acgccaatgc
    52981 tctgaacatg tagcgcgagg ctttcagggt tgtacgaaaa gaagcaaggt atttcaaagg
    53041 ggcgaagctc cgcctcaggt gaccgatgga gtgtggttaa ggtagcggta aaagcgtgtt
    53101 accgcaatgt tctgtcacca ttgcaataat gcgtatcgct tagaaaccat tgccttaacc
    53161 aggaaagccg gagtagatgg acagttctca gtacgaaaaa taagaaatga ccaatctgcc
    53221 gacgcttgtt aatggttata tcctggcgtc ggcagatcgc ccagcagtct tatctgaaaa
    53281 aagagagagc caggttgtgt gaagggatgg atcaccagtc aaaacttttc accagcgtca
    53341 gctcgccagc attacgcatc ggtacaataa atgtttcctg tttctcattg accgatcctt
    53401 catcggtgat cagcgtcagt tgggcggtcg tcaattccgt ttcgctgcgc ccaccatagt
    53461 agttgatata cacctgatag cgcccgtgaa ctggtgctgg catggcgaaa atctcgggtc
    53521 cgtaccccgt cgtgacatcc atatccagtg caccactgtt tttcagcacg gtgttaccgt
    53581 accaggcgtg ttcgccatcg ggcgtgacaa catggagatc gagatcggta ttgtccgtat
    53641 cccacgagag aaccagccgt aaacgtgcac gaatagttcc tgtacccggc gttgagtaaa
    53701 actgcatttt ttgccggctt tgcccatccg ggctgatgac ctgcacgctg ttgctgcctt
    53761 cagtgaaaat ataagggcgt gcaaatgaac catcggattc aattctctgc ggcatactgg
    53821 caccgttgac caccagccgg ccctgctgaa ctttacccgc cgcagcataa ttctttattt
    53881 tgccgctgat ctgcgctgag atattttgat catctgccat gttgacggac gacgccgggt
    53941 aattgatcga ttgtgaaaaa ctggcatctt cgccctctgc cggatgccag ccagagagcg
    54001 gtgcgtcaat ctcgacttcc tgcacacctt cactgtgagc gacaggcgaa agtgccacca
    54061 gtaacaacgg aagaaaaatc tttcgcatag cattagtcca ttaataattg tcgggtcagc
    54121 gtttcgatat agttctcatc aagcccggtg ggataaccat caaaggccaa gtgcagatac
    54181 tcatgggtta aatccagccg atcctgaagc gtaaagaagt tacgaataaa cagccgtttc
    54241 tgctgccgat cggtataggg gaaacctgag actaaacgac agaccgcaaa cacgtctggt
    54301 tcgttgtacc ccgtctcgcc ttgtaatata cgccgccact gcggcatttt tttcgccagc
    54361 caggctttcg ctttgggtaa taactggcag gttgagcgtg gagctcccca gcggctaagg
    54421 ctgttgtcgg gataagcaaa cgcgagtatc tggtcgtaac gctccccctg ccccgcctgg
    54481 gccgtggctt gccgccagga aagtgtccct tcggtagcgc gactgccatg ataatgaacc
    54541 ggatcacctg catagatgag ctcctgcgtc caggcggtca tcgttcgcgc ccccgtggtc
    54601 gctggcgaag cagaaacacg ctgcgtggcg ctgctgccgg ggatggtcag gcaatcacct
    54661 tcccgattgg cgttttgctg taaaaaagta cgtatcgcca cagtcatggc tttcgctgcc
    54721 tcaggtggcg tacttttcgc ctcgcgatcc agaactctgg caacgtactc ttcacgatcg
    54781 agatgcgact gcaatttcag tttgcccttt tcgctcagta aggtcgtctc accgtggctg
    54841 acaaacgtaa tgtggttacc attagtaaac gtcacacgat agcgcccatt aagtacgctg
    54901 ggttttactg ccgtcgtact cttttccgct gtgattttct tcagcggata gcgggcaaac
    54961 agctccacct ctacacattg cccactggca acctgcgtcg gcactggcaa aacccgatta
    55021 agcaccgtcg cataacgggt tagtaccgtt ttgctggtgc cactgccagt aacccacagc
    55081 ggtgtaccat cggttaacca gccagcaaaa ccaccctgac ggatttcctg tttatcatcg
    55141 gcaaaccagc tccaggtttt cacccgtacc cgactaccga gcatggatga gacaccgatt
    55201 ttcgcctcgt ccagcaccac atccagtaag acttcctgag ctttgttttg cgcaggtaac
    55261 gtcgctaatg aatcaagcag cgatttaacc gttaccgagg tttctggctt cagcgttgta
    55321 agaaacgcta accacgcagg tgcctgacgc tgttgccagt attgccccca cacatctgcg
    55381 ccaatatgca accgctgcgg cgcaaaatag agcccacagg aacgtaccag cgcggtatcg
    55441 cgcgttatgc tctccccagc ctgacaacaa taaacctctt ccggggagtt tccgcgacac
    55501 tggtagggct gctccggttg atgggtatcc tccagccagg cgtaaacata cagtttccac
    55561 aaactcccta acggcgtggt gagcgagtca ggtaatgctg agactttggt cacgcctgat
    55621 gacgacagct gatacagttg atcgtgctgc gcaccgcgca gcaccagttg taacggcgtc
    55681 tcctccgcaa gcgttggcag agtaaccaac gccaatagcc agacgattct gcgccagttc
    55741 acttatttca cctgcatccc ggtccattca ctccctgccg cgacactttg ctgcgcaggt
    55801 gcataggaac gcacataacg agcaggaggc agaacgaatt gccctttttg cgagaagcgc
    55861 agcaaatggc ggaaagtgac cgttcccgtc agttctttca ccggcaccat ataggccagt
    55921 tcgcccattt cattacgcgc tttttccagc aattgcccct gctgtttcgc ggcgttgggt
    55981 ttattgactg aaatgcccca tgttgtgcgc tcaacgtcgg ctcccggcgg gagcggtact
    56041 tccacctgac catagcgcag aactgcatcc tgctcgcagg taagcgtaat ttcatcgaga
    56101 tacagcgcat cgctgtcaat ctcattgctg gtcaccggct gcagagtaaa gctcatctct
    56161 tcttcaccgg ggataagccg atacaactgg cgttcaacgg tcaccggaat gttactttgt
    56221 tgagccgttt ttgccggttc acgccagcgg acctgcacat tttgcggcga taattcgtca
    56281 ccaaaagaga gaatgtccgg cacaccctga ccaacccaac gccagtcttc gccccctcca
    56341 gttaacttat gtttagccca tgcgcccgca ggcgcaggca aaacaactgg aggcattgtc
    56401 gccatatatt tcgccagcca gttcatggcc agcgcgcgct caatagtgga ttgctcagcg
    56461 gtcaaaccat taaaatagcg gccgtctggg tagcgtcacc accgctattg agcagcacaa
    56521 ccgtgtgcgc cagcggctga ttgctgtttg ccgctttatc ccacgcatat tgctgaacct
    56581 gtggcatcac cgtggacttc aactgcgctt ttttcagcaa cgtcatggtt aagacgtttg
    56641 ccaccgcatc tgccagtgga gattccggtg tatcgaggat caggctgtca ttgatatcgc
    56701 ggatgtcttc ctcagagaaa tcttcatctt tagttccgcg ccgggcgata gcttcatccc
    56761 cccctttcaa caacgttttg cacggcagat tcatctcctg tgcccacgcc agcaccagcg
    56821 cccgatgcaa taacggcatg ttatccgcct gctcggcata actgtcgagc atatgctgcc
    56881 agtattccgg ttgttgcgtt acgccgagcg cctggctggc ctgccaatcg gcgtaccatg
    56941 cccatgccgt aaggaaggcg tcaccattgc catcttcacc ccaccaggta aagcgcgctc
    57001 cgggccccgc cagttgcatc agccgcagac ggttatcctg aatcatctga cgaatgtcgt
    57061 tagcggcggc actttgatga tcggcaagcg aacgccaggc gagactgagc gggatcagac
    57121 ggctaccggt gttgatcacc ccaccccacg gttcatcgac taacgcatca agattgttgc
    57181 ggaaaatctc ctgcggcgtt tcactacttt gtagccggat attactcgcc tgctcgggca
    57241 acatcagcgc gttctcgcca ccgccgagca tgacattttt ctgctgttca acgggccagc
    57301 tgttgtccac aaaagacagt tttgtgctaa tgctgtcctg cacttgccca ttttgttgca
    57361 gttctgcact taacaagcca gattgctgaa tattctgcgt cagcgaaata taattcgccc
    57421 ctttgtgcag cgtcagcgtc tggcgcatct cagcgcctgc aaatttagtc accagcgcca
    57481 ccggttcgtt atcctgctga ctgaagataa acagtcctgc cgccggtttg tcgcccacgc
    57541 gatacaccgt tggcatactc cacttcatgt agagattttt ttccgaacgc agataagcac
    57601 gcccctgccc gaccagcccg tcgccgttca tcccacgcgc ggtgatacgc cagcgggtta
    57661 acgaatcagg catcaggaac gtgaaatacg ctttgccttg tttatcggtt gtgagtgacg
    57721 gcatccatgc cgcggtatcc acctcttcac gccgtggacg ttcaagcatt tttactcgcc
    57781 gctcgctgcg gttagtcgcg ccaggcgcaa ccggctcgct ggagagcgcc tggtcatagc
    57841 tgataaacga cagactggag ctggtacgca cattgttacg ccccagcgga tagaaaaatt
    57901 tgccgatatt cggcgcgatt tctggttgca gcgcgtagat catttcatcg accacgccta
    57961 ccgttagctg cgcagaaaca ggtttacctt tcagcgacga ggttaattct gaagtggtca
    58021 acaaaaactg gccaccgagt tagagttttt ccagtatcga ttttccgatt cgtttggggg
    58081 taacccaccg ttatattcgt gcggtctta
//
LOCUS       DAETPZ010000017        55740 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.017, whole genome shotgun sequence.
ACCESSION   DAETPZ010000017 DAETPZ010000000
VERSION     DAETPZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.017"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            77..865
                     /gene="modE"
                     /locus_tag="K0W59_001152"
     CDS             77..865
                     /gene="modE"
                     /locus_tag="K0W59_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HBI3022278.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNAATSLQQGQNVTAYFNADSVIIATLC"
     gene            933..2405
                     /gene="modF"
                     /locus_tag="K0W59_001153"
     CDS             933..2405
                     /gene="modF"
                     /locus_tag="K0W59_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HBI3022279.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            2666..3682
                     /gene="galE"
                     /locus_tag="K0W59_001154"
     CDS             2666..3682
                     /gene="galE"
                     /locus_tag="K0W59_001154"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBI3022280.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            3692..4738
                     /gene="galT"
                     /locus_tag="K0W59_001155"
     CDS             3692..4738
                     /gene="galT"
                     /locus_tag="K0W59_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBI3022281.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            4742..5890
                     /gene="galK"
                     /locus_tag="K0W59_001156"
     CDS             4742..5890
                     /gene="galK"
                     /locus_tag="K0W59_001156"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBI3022282.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            5884..6924
                     /gene="galM"
                     /locus_tag="K0W59_001157"
     CDS             5884..6924
                     /gene="galM"
                     /locus_tag="K0W59_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HBI3022283.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYTNRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            7127..7879
                     /gene="gpmA"
                     /locus_tag="K0W59_001158"
     CDS             7127..7879
                     /gene="gpmA"
                     /locus_tag="K0W59_001158"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBI3022284.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(8046..9098)
                     /gene="aroG"
                     /locus_tag="K0W59_001159"
     CDS             complement(8046..9098)
                     /gene="aroG"
                     /locus_tag="K0W59_001159"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HBI3022285.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYAIRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            9414..9794
                     /locus_tag="K0W59_001160"
     CDS             9414..9794
                     /locus_tag="K0W59_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HBI3022286.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            9908..10849
                     /gene="zitB"
                     /locus_tag="K0W59_001161"
     CDS             9908..10849
                     /gene="zitB"
                     /locus_tag="K0W59_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HBI3022287.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(10846..11565)
                     /gene="pnuC"
                     /locus_tag="K0W59_001162"
     CDS             complement(10846..11565)
                     /gene="pnuC"
                     /locus_tag="K0W59_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HBI3022288.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(11603..12646)
                     /gene="nadA"
                     /locus_tag="K0W59_001163"
     CDS             complement(11603..12646)
                     /gene="nadA"
                     /locus_tag="K0W59_001163"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBI3022289.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKTRADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAVTLRG"
     gene            complement(12895..12970)
                     /locus_tag="K0W59_001164"
     tRNA            complement(12895..12970)
                     /locus_tag="K0W59_001164"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12935..12937),aa:Lys,seq:ttt)
     gene            complement(12974..13049)
                     /locus_tag="K0W59_001165"
     tRNA            complement(12974..13049)
                     /locus_tag="K0W59_001165"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13014..13016),aa:Val,seq:tac)
     gene            complement(13101..13176)
                     /locus_tag="K0W59_001166"
     tRNA            complement(13101..13176)
                     /locus_tag="K0W59_001166"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13141..13143),aa:Lys,seq:ttt)
     gene            complement(13179..13254)
                     /locus_tag="K0W59_001167"
     tRNA            complement(13179..13254)
                     /locus_tag="K0W59_001167"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13219..13221),aa:Val,seq:tac)
     gene            complement(13391..13466)
                     /locus_tag="K0W59_001168"
     tRNA            complement(13391..13466)
                     /locus_tag="K0W59_001168"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13431..13433),aa:Lys,seq:ttt)
     gene            complement(13631..14422)
                     /gene="cpoB"
                     /locus_tag="K0W59_001169"
     CDS             complement(13631..14422)
                     /gene="cpoB"
                     /locus_tag="K0W59_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HBI3022290.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAHAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(14432..14953)
                     /gene="pal"
                     /locus_tag="K0W59_001170"
     CDS             complement(14432..14953)
                     /gene="pal"
                     /locus_tag="K0W59_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HBI3022291.1"
                     /translation="MQLNKVLKGLMIALSVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(14988..16280)
                     /gene="tolB"
                     /locus_tag="K0W59_001171"
     CDS             complement(14988..16280)
                     /gene="tolB"
                     /locus_tag="K0W59_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HBI3022292.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(16413..17654)
                     /gene="tolA"
                     /locus_tag="K0W59_001172"
     CDS             complement(16413..17654)
                     /gene="tolA"
                     /locus_tag="K0W59_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HBI3022293.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(17719..18147)
                     /gene="tolR"
                     /locus_tag="K0W59_001173"
     CDS             complement(17719..18147)
                     /gene="tolR"
                     /locus_tag="K0W59_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HBI3022294.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(18151..18843)
                     /gene="tolQ"
                     /locus_tag="K0W59_001174"
     CDS             complement(18151..18843)
                     /gene="tolQ"
                     /locus_tag="K0W59_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HBI3022295.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(18840..19244)
                     /gene="ybgC"
                     /locus_tag="K0W59_001175"
     CDS             complement(18840..19244)
                     /gene="ybgC"
                     /locus_tag="K0W59_001175"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBI3022296.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQHIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(19394..19687)
                     /gene="ybgE"
                     /locus_tag="K0W59_001176"
     CDS             complement(19394..19687)
                     /gene="ybgE"
                     /locus_tag="K0W59_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HBI3022297.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(19687..19800)
                     /gene="cydX"
                     /locus_tag="K0W59_001177"
     CDS             complement(19687..19800)
                     /gene="cydX"
                     /locus_tag="K0W59_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HBI3022298.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(19815..20954)
                     /gene="cydB"
                     /locus_tag="K0W59_001178"
     CDS             complement(19815..20954)
                     /gene="cydB"
                     /locus_tag="K0W59_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBI3022299.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTGGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(20970..22538)
                     /gene="cydA"
                     /locus_tag="K0W59_001179"
     CDS             complement(20970..22538)
                     /gene="cydA"
                     /locus_tag="K0W59_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HBI3022300.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILIANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(23387..26065)
                     /locus_tag="K0W59_001180"
     CDS             complement(23387..26065)
                     /locus_tag="K0W59_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBI3022301.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGFRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLEPLEPLEPLEPLEPLATLAWTLGFE
                     YHHGLLEKIWKEILKNHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIAD
                     NMPQSDADKLVLFNLMPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPG
                     LIDRQIVHYGNYDPFMEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENA
                     FWQIALNEDGTLQLVDKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQ
                     CEIIHEAWQSRAVIRYEMAVPRNLQQRSARQSTGRVGVELVVTLSHNNRRIDVDINLD
                     NQADDHRLRVLIPTSFNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNML
                     NYAALQEGRNGMAVFSEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGI
                     KMPVPDSQLRGLLSCRLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKA
                     GFNVPESYSLLKMPPVGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISC
                     SETMMDEHITTEENQGSNLSGPFLPGQSRTFSYRLA"
     gene            complement(26083..27999)
                     /gene="mngA"
                     /locus_tag="K0W59_001181"
     CDS             complement(26083..27999)
                     /gene="mngA"
                     /locus_tag="K0W59_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBI3022302.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWESVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYLVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNA
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMQ"
     gene            28169..28891
                     /gene="mngR"
                     /locus_tag="K0W59_001182"
     CDS             28169..28891
                     /gene="mngR"
                     /locus_tag="K0W59_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HBI3022303.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(28995..29864)
                     /gene="sucD"
                     /locus_tag="K0W59_001183"
     CDS             complement(28995..29864)
                     /gene="sucD"
                     /locus_tag="K0W59_001183"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HBI3022304.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(29864..31030)
                     /gene="sucC"
                     /locus_tag="K0W59_001184"
     CDS             complement(29864..31030)
                     /gene="sucC"
                     /locus_tag="K0W59_001184"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HBI3022305.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(31124..32341)
                     /gene="odhB"
                     /locus_tag="K0W59_001185"
     CDS             complement(31124..32341)
                     /gene="odhB"
                     /locus_tag="K0W59_001185"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HBI3022306.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(32356..35157)
                     /gene="sucA"
                     /locus_tag="K0W59_001186"
     CDS             complement(32356..35157)
                     /gene="sucA"
                     /locus_tag="K0W59_001186"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HBI3022307.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(35458..36174)
                     /gene="sdhB"
                     /locus_tag="K0W59_001187"
     CDS             complement(35458..36174)
                     /gene="sdhB"
                     /locus_tag="K0W59_001187"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HBI3022308.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(36190..37956)
                     /gene="sdhA"
                     /locus_tag="K0W59_001188"
     CDS             complement(36190..37956)
                     /gene="sdhA"
                     /locus_tag="K0W59_001188"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HBI3022309.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(37956..38303)
                     /gene="sdhD"
                     /locus_tag="K0W59_001189"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(37956..38303)
                     /gene="sdhD"
                     /locus_tag="K0W59_001189"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HBI3022310.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(38297..38701)
                     /gene="sdhC"
                     /locus_tag="K0W59_001190"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(38297..38701)
                     /gene="sdhC"
                     /locus_tag="K0W59_001190"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBI3022311.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            39082..39189
                     /locus_tag="K0W59_001191"
     CDS             39082..39189
                     /locus_tag="K0W59_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022312.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            39395..40678
                     /gene="gltA"
                     /locus_tag="K0W59_001192"
     CDS             39395..40678
                     /gene="gltA"
                     /locus_tag="K0W59_001192"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HBI3022313.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            41069..41635
                     /locus_tag="K0W59_001193"
     CDS             41069..41635
                     /locus_tag="K0W59_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBI3022314.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            41686..44145
                     /locus_tag="K0W59_001194"
     CDS             41686..44145
                     /locus_tag="K0W59_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBI3022315.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWYSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQY
                     CSLHLPEHMEDTANRLILPCH"
     gene            44160..44891
                     /locus_tag="K0W59_001195"
     CDS             44160..44891
                     /locus_tag="K0W59_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBI3022316.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            44888..45949
                     /locus_tag="K0W59_001196"
     CDS             44888..45949
                     /locus_tag="K0W59_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3022317.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIHVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            46101..47147
                     /locus_tag="K0W59_001197"
     CDS             46101..47147
                     /locus_tag="K0W59_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBI3022318.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(47144..47935)
                     /gene="nei"
                     /locus_tag="K0W59_001198"
     CDS             complement(47144..47935)
                     /gene="nei"
                     /locus_tag="K0W59_001198"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HBI3022319.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(47971..48705)
                     /gene="pxpA"
                     /locus_tag="K0W59_001199"
     CDS             complement(47971..48705)
                     /gene="pxpA"
                     /locus_tag="K0W59_001199"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HBI3022320.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKETQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(48695..49627)
                     /gene="pxpC"
                     /locus_tag="K0W59_001200"
     CDS             complement(48695..49627)
                     /gene="pxpC"
                     /locus_tag="K0W59_001200"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HBI3022321.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(49621..50277)
                     /gene="pxpB"
                     /locus_tag="K0W59_001201"
     CDS             complement(49621..50277)
                     /gene="pxpB"
                     /locus_tag="K0W59_001201"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBI3022322.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(50300..51043)
                     /gene="ybgI"
                     /locus_tag="K0W59_001202"
     CDS             complement(50300..51043)
                     /gene="ybgI"
                     /locus_tag="K0W59_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HBI3022323.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLENDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            51314..52795
                     /gene="dtpD"
                     /locus_tag="K0W59_001203"
     CDS             51314..52795
                     /gene="dtpD"
                     /locus_tag="K0W59_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HBI3022324.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTLF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(52945..54363)
                     /gene="phrB"
                     /locus_tag="K0W59_001204"
     CDS             complement(52945..54363)
                     /gene="phrB"
                     /locus_tag="K0W59_001204"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HBI3022325.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(54360..54869)
                     /locus_tag="K0W59_001205"
     CDS             complement(54360..54869)
                     /locus_tag="K0W59_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HBI3022326.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            54998..55315
                     /locus_tag="K0W59_001206"
     CDS             54998..55315
                     /locus_tag="K0W59_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HBI3022327.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGMARYYSLHLK
                     GDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELREELYQVKQVVKGMRDGMFSVL
                     NES"
     gene            complement(55370..55690)
                     /locus_tag="K0W59_001207"
     CDS             complement(55370..55690)
                     /locus_tag="K0W59_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022328.1"
                     /translation="MPGYRTWIEISLIDELKPSYPFRLEEYAMIGHSPYANECNKDEA
                     KFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELKTRLSTLHVNLEQFINSSEDD
                     EYTL"
BASE COUNT        13635 a        14859 c        13645 g        13601 t
ORIGIN      
        1 gcactcccct tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac aataagactt
       61 ttgccaggac attgttatgc aggccgaaat ccttctcacc cttaagctcc aacaaaaatt
      121 attcgccgac ccgcgccgca tttcgctact aaaacacatt gcgctttccg gttccattag
      181 ccagggagcg aaagatgccg gtatcagcta taaaagcgcc tgggatgcca ttaacgagat
      241 gaatcagtta agtgagcata ttctggtcga gcgcgcaaca ggcggtaaag gtggcggagg
      301 cgcagtactg acccgctatg gtcagcgact gattcagctc tatgacttac tggcgcaaat
      361 ccagcaaaaa gcctttgatg tgttaagtga tgatgacgcc ctgccgctga acagcctgct
      421 ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt aaccagtggt tcggtaccat
      481 caccgcccgc gatcatgatg acgttcaaca gcatgttgat gtcttactgg ctgacggaaa
      541 aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc tggatgaagg
      601 caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc attactcagg acgaagcggt
      661 cgcacaaaac gccgacaacc aattaccggg tattattagt catattgagc gcggcgcaga
      721 gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt
      781 aaatgcagcg acttctcttc agcaagggca gaatgtcacg gcctacttta atgccgacag
      841 cgtgattatc gccacgctgt gctaagcgtg ttgacaattt gttatgaaac acgtatccct
      901 gtcagtaatc gctgcacaaa atgggatata aaatgtcatc gttgcaaatt ttgcaaggca
      961 cgtttcgtct tagcgacaca aaaacgctgc aattgcctca gctaacgtta aacgcgggtg
     1021 atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc ggcgctggcc cgcgcgctgg
     1081 cgggggaact tccgcttttg aaaggtgaac ggcaaagcca gttttcccac atcactcgtc
     1141 tctccttcga gcaattgcaa aagctcgtca gcgacgaatg gcaacggaat aacaccgata
     1201 tgctcggccc tagcgaagat gacaccggac gcactacggc tgagattatt caggatgaag
     1261 taaaggatgc accgcgttgc atgcaactgg cgcagcagtt cggtattacc gccctcctcg
     1321 accgacgctt taaatacctt tccactggcg agacgcgaaa aaccctgctg tgtcaggcgc
     1381 tgatgtcgga gcctgacttg ttgattcttg atgagccgtt cgatggcctg gatgttgcct
     1441 cacgtcagca gctggctgag cgactcgcct cgttacatca gtccggtatt actttggtac
     1501 tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca gtttgctggc gtgctggcgg
     1561 attgtacgtt agcggaaact ggcgctaaag aggaactgct ccagcaagca ctcgtcgcgc
     1621 aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc ggagccggat gaaccttcag
     1681 cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct gaacaatggc gtggtttctt
     1741 ataatgatcg ccccattctt aataacctta gctggcaggt gaatccaggc gaacactggc
     1801 aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt aagcctgatt actggcgatc
     1861 atccgcaagg ttacagcaac gatttgacgc ttttcggccg acgtcgcggc agcagcgaaa
     1921 ccatctggga catcaaaaag catatcggtt acgtcagcag tagtttgcat ctggattacc
     1981 gggtcagcac taccgtgcgt aatgtgattc tttctggcta ttttgattcg attggcattt
     2041 atcaggccgt ttcggatcgc cagcaaaaac tggtgcagca gtggctggat attctcggca
     2101 ttgataaaca cacggctgac gctccgttcc atagtctttc ctggggacag cagcgtctgg
     2161 cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct tattctcgat gaaccactac
     2221 aggggcttga tccgctcaat cgccaactta tccgccgttt tgttgatgtg ctgattagcg
     2281 aaggtgaaac gcaattgttg tttgtttcgc accacgctga agatgcgcct gcctgtatta
     2341 cccatcgcct tgagttcgtg ccggacggtg gactctatcg ctatgtgctg acaaaaatat
     2401 attgagtcgg tagtgctgac cttgccggag gcggcctaag cgccctctcc ggccaacggt
     2461 tcgacgcatg caggcatgaa accgcttctt ttttcagata aaaagcgcaa tcattcataa
     2521 accctctgtt ttataatcac ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg
     2581 attccactaa tttattccat gtcacacttt tcgcatcttt gttatgctat ggttatttca
     2641 taccataagc ctaatggagc gaattatgag agttctggtt accggtggta gcggttacat
     2701 tggaagtcat acctgcgtgc aattactgca aaacggtcat gatgtcatca ttcttgataa
     2761 cctctgtaac agtaagcgca gcgtactgcc tgttatcgag cgtttaggcg gcaaacatcc
     2821 aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg accgagatcc tgcacgatca
     2881 cgctatcgac accgtgatcc acttcgccgg gctgaaagcc gtcgccgaat cggtacaaaa
     2941 accgctggaa tattacgaca acaatgtcaa cggcactctg cgcctgatta gcgccatgcg
     3001 cgccgctaac gtcaaaaact ttatttttag ctcctccgcc accgtttatg gcgatcagcc
     3061 ccaaattcca tacgttgaaa gcttcccgac cggcacaccg caaagccctt atggcaaaag
     3121 caaattgatg gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg actggagcat
     3181 tgccctgctg cgctacttca acccggttgg cgcgcatccg tcgggcgata tgggcgaaga
     3241 tccgcaaggc attccgaata acctgatgcc atacatcgcc caagttgctg taggccgtcg
     3301 cgactcgctg gcgatttttg gtaacgatta tccgaccgaa gatggtactg gcgtacgcga
     3361 ttacatccac gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa
     3421 caagccaggc gtacacatct acaacctcgg tgctggcgta ggcagcagcg tgctggacgt
     3481 ggttaatgcc ttcagcaaag cctgcggcaa accggttaac tatcattttg caccgcgtcg
     3541 cgaaggcgac cttccggcct actgggcgga cgccagcaaa gccgaccgtg aactgaactg
     3601 gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc tggcactggc agtcacgcca
     3661 tccacaggga tatcccgatt aaggaacgac catgacgcaa tttaatcccg ttgatcatcc
     3721 acatcgccgc tacaacccgc tcaccgggca atggattctg gtttcaccgc accgcgctaa
     3781 gcgcccctgg cagggggcgc aggaaacgcc agccaaacag gtgttacctg cgcacgatcc
     3841 agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc ccgattacac
     3901 cgggacttac gttttcacta atgactttgc ggccttgatg tctgacacgc cagatgcgcc
     3961 agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg
     4021 cttttcaccg gatcacagta aaacgttgcc agaactgagc gttgcggcat tgacggaaat
     4081 cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt
     4141 ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg catccgcacg gacagatttg
     4201 ggcaaatagc ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa aagaatattt
     4261 tgccgaacag aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg cagacggtag
     4321 ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg ccttactggg ctgcctggcc
     4381 gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc
     4441 ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc agtcgttatg acaacctctt
     4501 ccagtgctcc ttcccctact ctatgggctg gcacggcgca ccatttaatg gcgaagagaa
     4561 tcaacactgg cagctgcacg cgcactttta tccgcctctg ctgcgctccg ccaccgtacg
     4621 taaatttatg gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca
     4681 ggcagcagag cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga
     4741 aatgagtctg aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac
     4801 tcacaccatt caggcgcctg gccgcgtgaa tttgattggt gaacacaccg actacaacga
     4861 cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg caccacgcga
     4921 tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat cagctcgacg agttttccct
     4981 caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg aactacgttc gtggcgtggt
     5041 gaaacatctg caactgcgta acaacagctt cggcggtgtg gacatggtga tcagcggcaa
     5101 tgtgccgcag ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt
     5161 attgcagcag ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga
     5221 agcagaaaac cagtttgttg gctgtaactg cgggatcatg gatcagctaa tttccgcact
     5281 cggcaagaaa gatcatgcct tgctgattga ctgccgctca ctggggacta aagcagtttc
     5341 catgccgaaa ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg
     5401 cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc
     5461 agctctgcgc gatgtcacca ttgaagagtt caacgctgtt gcacatgagc tggacccaat
     5521 cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg aagctgccag
     5581 cgcgctggag caaggcgacc tgaaacgtat gggcgagttg atggcggagt ctcatgcctc
     5641 tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa
     5701 agctgtgatt ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat
     5761 cgtcgcgctg atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata
     5821 tgaagcaaaa acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg
     5881 acagtgctga acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttaact
     5941 ttgcgtaaca acgcagggat ggtagtcacg ctgatggact ggggtgcgac tttactttcc
     6001 gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg tgccagtccg
     6061 gaatgctatc aggatcaggc tgcgtttctg ggggcctcta ttggtcgtta taccaaccgc
     6121 atcgccaata gtcgttatac ctttgacggt gaaaccgtga cgctttcgcc aagccagggc
     6181 gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca gattgtgaac
     6241 cagaacgatc gtcaggtgct gtttgccctg agttcagatg atggcgatca gggcttcccg
     6301 ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat ctccattact
     6361 tatcgcgcca cagttgataa accttgcccg gtgaatatga ctaatcacgt ctatttcaat
     6421 cttgacggcg agcagtctga cgtgcgcaat cacaagttgc agattctggc ggacgaatat
     6481 cttccggttg atgaaggcgg cattccgcac gacggcttga aatctgtcgc cggaacgtct
     6541 tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc
     6601 aaagtgaaag gttacgatca cgcattcttg ttacaggcca aaggcgatgg caagaaagtg
     6661 gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga aggtctacac caccgctccg
     6721 gctcttcaat tctactccgg caacttcctc ggcggcacac cgtcgcgggg aaccgaacct
     6781 tacgccgact ggcaagggct ggcgttggaa agcgagtttc tgccggacag cccgaaccac
     6841 cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc cagcctgacg
     6901 gaatatcagt ttattgctga gtaatgttaa gccctcgtca tgagggcttt atcttcatat
     6961 tgttcaaatc accagcaaac accgacatat ttgcaactca atattcacaa taaccttaca
     7021 ctgcgccact attttcgcta tggttatgcg taagcattgc tgttgcttcg tcacggcaat
     7081 ataatgagaa ttattatcat taaaagatga tttgaggagt aagtatatgg ctgtaactaa
     7141 gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt tcaccggttg
     7201 gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca aaagcagcag gtaagctgct
     7261 gaaagaggaa ggttacagct ttgactttgc ttacacttct gtgctgaaac gcgctatcca
     7321 taccctgtgg aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg agaaatcctg
     7381 gaaactgaac gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg aaactgctga
     7441 aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt tttgcagtga ctccgccgga
     7501 actgactaaa gatgatgagc gttatccggg tcacgatccg cgttacgcga aactgagcga
     7561 gaaagaattg ccgctgacgg aaagcctggc gctgaccatt gaccgcgtga tcccttactg
     7621 gaatgaaacc attctgccgc gtatgaagag cggtgagcgc gtaatcatcg ccgcacacgg
     7681 taactccctg cgtgcgctgg tgaaatatct tgataacatg agcgaagaag agattcttga
     7741 gctgaatatc ccgactggcg tgccgctggt gtatgagttc gacgagaatt tcaaaccgct
     7801 gaaacgctat tatctgggta atgctgacga gatcgcggcg aaagcagcag ccgttgcgaa
     7861 ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg agaaagccga ctgcaagtga
     7921 gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat tgcctgatgc gacgctgcgc
     7981 gtcttatcag gcctgtggtg attcatcgga tacgccactc tgacggcgca tccgacaatt
     8041 aaaccttacc cgcgacgcgc ttttactgca ttcgccagtt gacgtaacag agcatcggta
     8101 tcttcccagc caatgcaggc atcggtgatg ctcttaccgt aggccagcgg ctccccgcta
     8161 tcgaggctct gattgccttc caccagatgg ctttccacca tcacgccaat aatggccttt
     8221 tcgccaccgg caatctgctg gcaaacgtca gcacaaacat ccatctgctt tttgaattgt
     8281 ttggacgaat tagcatggct gaaatcgatc atcacctgag ctggcagacc tgctttgttc
     8341 agcccttctt tcacttcagc aacgtgcttc gcgctgtagt taggctcttt accgccgcgc
     8401 agaatgatat ggcaatcgcc gttaccactg gtattcacaa tcgccgaatg cccccattta
     8461 gttacggaca ggaagcagtg cggcgcaccg gcggcattaa tggcatcgat agccacttta
     8521 atcgtaccgt ctgtgccatt tttaaaacct accggacaag aaagaccaga cgccagttcg
     8581 cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc cccagctcat caggtcagcg
     8641 agatactgag gagtgatcat atcgagaaac tcacccgccg ctggcagacc gctgtcgtta
     8701 atatcaagca gcaatttacg ggctatacgc agaccgtcgt tgatctggaa gctgttatcc
     8761 atatgcggat cgttaatcaa ccctttccag cccaccgtgg tacgcggctt ttcaaaatag
     8821 acgcgcatta cgatttccag ctcatctttc agctcttcac gcagcgccag caagcgaatg
     8881 gcatactctt tagccgcgac aggatcatga attgagcacg ggccaatcac caccaacagg
     8941 cgatcatcat tacctttcag gatcttatgg atcgcttttc gggcatgagc aaccgtattc
     9001 gcggcatttt cagttgcggg gaatttttcc agcaatgcga caggaggaag taactctttg
     9061 atttctttga tgcgtaaatc gtcgttctga taattcatgt ctgttccagt gtcgccatac
     9121 ttatcttagt gaatgcaata cttccaatct atatcttccg tcagaatgtg taaacggggt
     9181 tttacactat gaacggatta atcctagaat tagcaagaaa aacgccagat tgtcgcgaaa
     9241 aacgagatct ttcctacaat ttctaactgt aactcctttg tttgttaatt atttcaagat
     9301 tctctgctgc gtttcataaa ctggctgaaa tcttaaacca atgccttata ttcacctgca
     9361 aatgcactgt tggaagaggt tatccgacat aacgaccata acaggagcat cctatgaaaa
     9421 tgacaaaact ggccacatta tttctgactg ccactctaag ccttgccagc ggtgccgcac
     9481 tggctgccga tagcggagcg caaactaata acggccaggc aaacgccgca gctgatgcgg
     9541 gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa taacgtcgac aataacgggg
     9601 taaataccgg ttctggcggc acaatgctgc attcggatgg ttcttcaatg aacaatgacg
     9661 gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa agatggtcga tgtccggata
     9721 tcaataaaaa ggtacaaacc ggtgatggca tcaataatga tgtcgatacc aaaaccgacg
     9781 gtaccacgca gtaaccctgc aaaatagcca ataatgggag agcggagtct ctccctttta
     9841 atttttcact tctaaaagca gtatgatgtc ataaagttaa gtgagaataa ttaagggatg
     9901 actcgtcatg gcgcactcac actcacacac gtcctcacac ctgccagaag ataataatgc
     9961 tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt atgctggtag aagtcgttgg
    10021 tggttttctt tctggttctc tggcattgct ggccgatgcg ggtcatatgt tgaccgatac
    10081 tgccgccctg cttttcgccc tactcgccgt gcaattttca cgtcgtcctc ccaccattcg
    10141 ccacactttc ggttggctaa gactgaccac gctcgcggcg tttgtgaatg ccatcgccct
    10201 cgtggtaatt accattttga ttgtctggga ggcgatagaa cgcttccgca cgccgcgtcc
    10261 ggtcgaggga ggcatgatga tggcaattgc cgtggctggg ttgctggcaa atatactttc
    10321 tttctggtta cttcatcacg gcagcgaaga gaaaaacctc aacgtacgag cagcggcact
    10381 gcatgtgctc ggggacctgc tgggttcggt tggggctatt attgccgcgt tgattattat
    10441 ctggactggc tggacacctg ctgaccccat tctctcgata ctggtgtcgc ttctggttct
    10501 gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa ttacttgaag gtgcaccggt
    10561 atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg gaaatcccgg aagtccgcaa
    10621 tgtgcaccat gtacatgtat ggatggtagg cgagaagccg gtgatgacgt tgcatgtgca
    10681 ggtgatcccg ccacacgatc acgatgcctt gttggatcag atccaacact acctgatgga
    10741 tcactatcag attgagcacg ccaccattca gatggaatat caaccttgtc atgggccaga
    10801 ctgccacctg aatgaagggg tgtctggcca ttcacatcat caccattaat gggacagcgc
    10861 gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg ctgccgttga gcgcaataaa
    10921 ggtcaggatg atgtactcca gagacatggc gtaaacgccc tgaagtgcaa aaataacgac
    10981 gctaatcacg ttaataatca cccacaacag ccagttttcc acatacttac gcgtcatcag
    11041 aatcattgcc acgatagata acaccatcat gcatgaatcc cagaacggga aagcgtccgg
    11101 ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc atgatcatga ctgccacgcg
    11161 ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc agaccaatcg aaacaacgca
    11221 aaccgccaac cagctgagtg ccttcggcaa tggcaaccag cgaattttca actccgcctc
    11281 gttctgactg gtttgtcgcg accacgcata ccagccgtaa atattcgcgg caaagaaaaa
    11341 cacctgtaat agcaggctgg catacagctg aatctgaaag aaaataatgc caaacaaggt
    11401 aacgttgatc aggccaaaga agtagttgct gatcttctcc agactggcaa ggccaataca
    11461 cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag agatcataac cgcccgcccc
    11521 tattggtata tgtaccagga tattctgcac actaaaaaaa tccatctttc ccccaaagcg
    11581 taacgttacg ccttattatt cgttatccac gtagtgtaac cgcaaaatcc agcatacgat
    11641 tgagcggcac cagcgccctc tctcgcagcc tctcatcgac atgaacctcg tgattacttc
    11701 cttcctgttc taatgcctct gcgatagcct gaaggccatt catggccatc cacggacaat
    11761 gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg tgcttccagt aactctttat
    11821 ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc ggttgccaca ataagcctct
    11881 gatgaggcaa tgcttttgca gcagcgatca gttgactggt ggaaccgacc gcatccgcca
    11941 tatcgacaat agcttgtggt gattctggat gcaccagtat ggcagcatcc gggtattctt
    12001 cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg cacaatgcag gcaccctgcc
    12061 agcataaaat gtctccaccc gtctgttttt gcacgtaacg ccccagatgt ttgtcgggtg
    12121 cccagatgat tttttcaccc aaactatcaa gatgatcaat aagttcgacg gcaatgcttg
    12181 aagttaccac ccaatctgcg cgcgttttta ccgcagcaga agtgttggcg tagacgacga
    12241 cagtacgatc gggatgggca tcgcaaaatg cgttaaattc ttcaacaggg cagccgagat
    12301 ccagtgaaca ctcaacctga agtgtcggca tcagaattgt tttttccgga ctgagaattt
    12361 tggcggtttc tcccataaat ctcaccccag cgactaacaa agtagaagcg ggatgctttg
    12421 caccgaagcg cgccatttcc agagaatcag aaatacagcc accggtttct tctgccagtt
    12481 gttgaatttc gggatcggta tagtagtggg caaccatcac cgcattacgt tcttttagca
    12541 gacgttttat cttctcgcgg taatacgctt tttcatcaat gcttaacggc gtcggcttcg
    12601 gggggaaagg ataaatcgcc gtgtctggat caaacattac gctcatctta ccatctcgtt
    12661 ttacaggctt aacgttaaaa ccgacattag cgtaacattc gcttttatcg gtgtaatttg
    12721 ttttatatgc taaacaagat agtcgaatgg tggggaaaag tcactcgaat tacgcctaat
    12781 tccaccgacg tagagttgct tgcacagtaa gctctgacga tgtcatcagg tttacaaagt
    12841 gaaattgagg atgcctgaat agcaaaaaag cgcctttagg gcgctttttt acattggtgg
    12901 gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt caactgctct accaactgag
    12961 ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc cgcccccctc cttgtaaggg
    13021 aggtgctctc ccagctgagc taatcacccg atactgcgct ggatactaca aactacttcc
    13081 accgaaccca ccgtaattag tggtgggtcg tgcaggattc gaacctgcga ccaattgatt
    13141 aaaagtcaac tgctctacca actgagctaa cgacccggtg gtgggtgatg acgggatcga
    13201 accgccgacc ccctccttgt aagggaggtg ctctcccagc tgagctaatc acccgacact
    13261 acgctggata ctacaactaa ctatgttagt ggtgggtcgt gcaggatgac tcgacttcgt
    13321 ctcgcccttc ggcgccgttg ctgatgcaac gttatccttc acattttact accgcttcca
    13381 ccttagcgat tggtgggtcg tgcaggattc gaacctgcga ccaattgatt aaaagtcaac
    13441 tgctctacca actgagctaa cgacccactt tttcgttgct ttcggtttgt ttgatatccc
    13501 gtggcaacgg cggcatatat tactgatttc agacttgagc gcaacaaaaa tttcgatgca
    13561 gatcactcaa ctgcttatga ttcgcacgac acgaccagaa ataatgcgac ttctggtcgt
    13621 gtgttatgca ttacatcgcg ttcagacgtt tttgtgcctg tttagcgcca tcagtaccag
    13681 ggtatttact gataacctgc tggtatacgg ctttcgcttt tgcggtgtca cctttgtcct
    13741 gcatgatgac gccgacttta aacatcgcat cagcagcctt tggtgacttc ggatagtttt
    13801 tcactaccga agcaaaatag tacgccgcat catctttttt acccttgttg tagtttaact
    13861 gacccagcca ataattggcg tttggcaggt aagttgaatc agggtaattt ttgatgaaat
    13921 tctgaaatgc caccattgcg tcatcctggc gggatttatc ctgcaccagc gcaatagctg
    13981 cattgtaatc cgtgtttgca tcgccgcttt tcaccggcgc gccagcattc gcagtaccag
    14041 catcagctgt cggcgtcgtt gatgccgccg caccgctttg atcgctgctg gttgattgcg
    14101 ccgctgcacc accgctgctg aggctgtcga tctgcaacag gatctgcttt tgccgctcca
    14161 cgacctgatt cagttgatac tgattttcct gaatctgacc acgcagggaa tcaatatcgg
    14221 attgattatc agaaagttgt tgctggagtt gggttaaaag ctggctgtga gcattagaaa
    14281 tacgctcaag ttgagtgacg cggtcttcga ccgagcctga gccgacacta ctgattggtg
    14341 catgagcaaa agcggcccag ggggccgcta taccaaccag taacgacaga ctcaatagtt
    14401 gatgtctgaa gttactgctc atgcaattct cttagtaaac cagtaccgca cgacggtttt
    14461 tggagtatgc tgcttcgtca tgacccagta ctgcaggttt ttctttaccg taagaaacga
    14521 tggagatctg gtctgcagaa acgcctttac cctgcaggta catcttaacg gcgttcgcac
    14581 gacgttcacc cagggagatg ttgtattccg gagtaccacg ttcgtccgcg tgaccttcta
    14641 cggtgacttt gtaagacggg ttgctacgca ggaagtttgc atgtgcatcc agcatttgag
    14701 cgaagtcaga acggatatcg tacttgtcca gatcgaagta aacgatgttg ttctgctgca
    14761 gctgttgcat ttgcagacga gcctgctctt cggaagacat gttgccgttg ccgccgttcg
    14821 catccatacc agtgccagca cccagcatgc cttcgctgcc gtcattgctg gcgttcttgt
    14881 tggaagaaca tgccgcaatt gccataacag acagagcaat catcagccct ttcagcactt
    14941 tgttcagttg catttcaatg attcctttac tattcaatta attattatca cagatacggc
    15001 gaccaggcag ggaatttgac ctgtccatca gttgccggaa gacgcgcttt gaaacgccca
    15061 tctgtagaaa ccaaattcag cacggatccc atcccctgag aagagctgta gattaccata
    15121 gtgccgttag gtgccagact tggcgtttca tccaggaacg tggacgacag aacttgtacg
    15181 cctcccgttg ccagatcttg tttggcaatg tgctgctgcc caccattgga gctgaccatt
    15241 accataaatt taccgtcgct gctgacatcc gcatcctggt tctgcgaacc ttcccaggta
    15301 atacgttgtg gcgcaccgcc gttgatattc actttataaa cctgcggacg accggcctgg
    15361 tcagaagtaa atgccaggtt ctggctatcc gggaaccagg tcggttcggt attgttactg
    15421 cgaccatcag tcacctggcg gatctgacca gaagccaaat ccattacgta caggttcaga
    15481 ctaccggttt tcgacaaggc gaatgccagt ttgctgccgt ctggcgagaa tgcaggtgca
    15541 ccgttgtgac gcgggaatga agccacctga cgtacagcgc catttgccag cgtctgaata
    15601 accagcgcgg aacgaccgct ttcgaaggtc acataagcca gtttagaacc gtctggtgac
    15661 cacgccggag acatcagcgg ctgcggtgaa cggtgaacga caaactggtt gtaaccgtca
    15721 tagtcagata cgcgcagttc atacgggaac tgaccgccgt tggtctgaac aacgtaggca
    15781 atacgggtac ggaacgcacc tttaatgccg gtcagttttt caaacacttc atcactggcg
    15841 gtatgaccag cataacgcag ccactgcttg ttcactttgt acgagttctg agcaagtaca
    15901 gtacccggtg cgccgccagt gtcaacaagt tgataagcaa cattgtaaga accatccgga
    15961 ttcggagtga cctgaccgac aactacagcg tcaatgccca gtgcggacca tgcagctggt
    16021 tgtacttcct gcgcactacc cggctgctgt ggcagacgag cgcgatctaa cggattaaat
    16081 ttaccgctgt tacgcaagtc agcagcaacg atgccgccaa tatcttcagg tgccgcacca
    16141 ggccccgccc actggaaagg aacaacacca ataggacgac cggaatctac accgctgtcg
    16201 atcacaatgc ggacttcagc atgcagaact gatgcccaca gtatgagaaa accaaatgct
    16261 actcgtaatg cctgcttcat catatctccc ttatctggac cgatggccca cgataattta
    16321 gcagaatgtt aacaaactaa aatacacaaa actaccagaa ccccgtggca accggtgcct
    16381 gatgttgacc gtccgaacag tcaacatcgc gattacggtt tgaagtccaa tggcgcgttt
    16441 ttgaacactt catatactgc ctggcttggt ggtttcggga tcttcgcaag tttagctgct
    16501 gccaacgcag cctgacaaag tgcgggatcg ccaccttcag gtttgatatc cagtaacata
    16561 ccatcgggtg ccagttttat gcgcagcgta caggttttgc ctgcatagga cgatgcgtca
    16621 tagaacttac tttcgatagc agatttaatc tgcccggcat agttattgat atcggcccct
    16681 gatgcgccat tgtttttagt attaccactc ccggcaggcg aagcattgtt ccctttcgcc
    16741 cctccccccg ttttcggtgc attcttacca gagcttagct caccgaaaat atcatctgcc
    16801 tctgcggcag cttttgctgc agcggctttt tcagctgctg cttttgccgc tgccgctttt
    16861 ttgtctgcgg cggctttttc tgccgctgct ttcttatcag ctgctgcctt atcagcagcc
    16921 gctttttctg ctgctttttt gtcggctgca gctttctctg ctgccgcttt cttatcagct
    16981 gcagcctttt cagcagccgc tttcttctca gcttctgcct tggctttttc agcagcttca
    17041 gttgccgctt tctttcttgc ttcagctgca gctgcttctg ccgcttccgc tttcttcttc
    17101 agtgccgcag cggctacctc ggcttttttc tgcgcttcgg ctgcggcttt ggcggcttct
    17161 gcttctgctt ttttcttcgc gtctgcagcc gctttcttcg cagcttcttc cgcagcttta
    17221 gcatctgccg ccgctttcgc tgccgccact tcagcttgct tctgctttaa ctcggcctgt
    17281 tttgcggctt cttcagcctg ctttttctgc tcctgagccg ctaaccgctc tttctcaagt
    17341 tgcttcaggc gttcctgttc agccgcttgt ttctcacgca gttcttcagc agcctgctgt
    17401 tccttcatct tgcgctgttc atcagaacgc ttcgcgcttg attcctggct ttgcatgcgt
    17461 ttgtactgct caactaccgc acctgaatca accatgacag cgtcgatgga cgaaccaccg
    17521 ccgcctccgg ctgaagcttc tatattctca tcgaacgaac tccagatcag cgccgcaaat
    17581 aagatgacat gcagcactgc tgaaataatt atcgcccgct tgagcttgtc gttttgttcg
    17641 gttgcctttg acactctcgg tttccaaaaa ctgttcgcct gttacccgct ctctttcaag
    17701 caagggaaac gcagatgttt agataggctg cgtcattaaa ccaaccgatt tcacacccgc
    17761 actatgtaac aagttcagtg ctttaattat ttcatcgtaa ggcacatctt ttgcgccacc
    17821 gatcagaaag accgttttcg ggttggcctt gaaacggctg gacacttccg ccaccacctg
    17881 ctctggtggt aaacgctcca ggcgatcttt ctcaaccacc acggtgtact gaccaatacc
    17941 agacacttca acaatcactg gcggattatc gttactgctc accgcctgtg attcagtagc
    18001 gtctggcaga tcgacctcca cgctctgggt gatgatgggc gctgtcgcca taaagatcag
    18061 caacagcacc agcagtacgt ccagcaacgg tacaatgttg atttcggact tgagatcgcg
    18121 acgacctcgt ccacgcgctc tggccatggc ttaccccttg ttgctctcgc taacggtaaa
    18181 cgcctggcgg tgcagaatcg cggtaaactc ttccataaag ttgtcgtaat tcagttccag
    18241 tttgtttacg cgctggttga gacggttata agccattacc gccggaattg cggcgaacag
    18301 accaattgcc gtcgcaatca acgcttctgc gataccgggc gcaaccattt gcagtgttgc
    18361 ttgttttacc gccccgaggg cgataaaggc gtgcatgatc ccccagaccg taccaaacag
    18421 accaatatac gggctgatgg agccaaccgt accgaggaac ggaatgtgcg tttccagatt
    18481 ttcaagttca cggttcatgg agatacgcat agcacgcgac gccccttcca ctacggcttc
    18541 cggcgcatgg ctattggcac gatgcaggcg cacaaactct ttgaacccgc tgtaaaagat
    18601 ttgttccgaa ccagtcagat tatcccgttt cccctgactc tcttgataga ggcgagagag
    18661 ttcgattcca gaccagaatt tatcttcaaa cgcttcggct tcacgcgccg ctgcgttaag
    18721 aatacgggtt cgctggataa taatggccca ggatgcgatt gaaaaaccaa tcaaaatcaa
    18781 catgataagt ttaaccagaa ggctagcctt caggaacaaa tcaaggatat tcatgtcagt
    18841 cactgcttaa actccgcgac aatagacttg ggaagcgcac gaggcttcat tttgagtggg
    18901 tcaacgcaaa caaccagaac ctctgcttca ttcagcaaag tattctcggc gttgacaata
    18961 tgttgcgtga aaaccaaaga ggtgccacgc attgatgtta tttcagtctg tatttcgagc
    19021 atatcgtcga gccgcgcagg tgcgtaatat tccaccgtca ttttacgtac cacaaaggca
    19081 acgcgttcag ccatcagcgc ctgctgactg aagtgatgat gacgcagcat ctctgtgcgt
    19141 gctctttcat aaaaagcgac gtaactggcg tggtacacca caccaccggc atcggtatct
    19201 tcatagtaga cgcgaaccgg ccatcgaaac agcgttgtat tcactttaca tcccggtaat
    19261 gcaacaaaag ttagagcttt taaacgctgc tactatacgc gcgcgattga ggtttgggaa
    19321 tgagggcaag ttaagggagc gtaaaaattt atgggcctgg agcggcccat aattttttaa
    19381 agagaattct gatttaaaag aagaagaaaa tcagcccgac aatgagaaca atatcggcaa
    19441 gcaatgggca aaaaatccct tgccaaagaa ccttctgcgg acgaaagccc acgccgtgaa
    19501 tcacaccagc acagacggcc cacatcagca ataaaccatg ccagatttcc agttcactgg
    19561 tcttcgcggc aaaacgagat gggtcccaaa acatacatcc tgctaacaga agcgccatca
    19621 cgaaggaaag cgcccgcagg gggcgcttgt ccattaccgc atacaaggtc gcgataatct
    19681 tactcatcag atgtcttctt gaccggcttt gcctgattcg acgtgttcaa gcgccagcgc
    19741 ggtgattacc ccaaacgaac aggcaagaag cgttcccaga atccatgcga aataccacat
    19801 ttttagctcc ttacttagta cagagagtgg gtgttacgtt caatatcttc tttggtgata
    19861 cgaccgaaca ttttccagta acaccaggcg gtgtagagca gaatgatcgg taccagaacc
    19921 accgcaaccc aggtcatgac gttaagcgtc agctggctgg aagttgcatc ccacattgtc
    19981 agacttgcgt tcatcatggt gctggacggc atcacaaacg ggaacattgc gataccggct
    20041 gtcaggatga tgcaggccag cgtcagggag gagaacacaa acgcccacgc ggctttatcc
    20101 atacgtgcag tcaggatggt cagcagcggc agaaccacac ccagtgccgg aatagcccac
    20161 agaattggcg tgttgttgaa gttaaccagc catgcgccag cttcacgaac cacttcttta
    20221 ttcagtgggt tagaggctgc gtaatggtcc attgtcgatt tcacgacata accatcgata
    20281 ccgtacatca cccatacgcc agccagtgcg aaacagacca gtgtcaccag cgcagccacc
    20341 tgagccgttg cacgggtacg caggtgcagt tcgcccccgg tacgcatttg cagataggtt
    20401 gcgccctgag taatgatcat ccctacgctc accacgcctg ccagcaggcc gaacgggtta
    20461 agcaactgga agaagttacc ggtgtagtac agacgcagat attcatcaac gttgaacggt
    20521 acgccctgca acaggttacc gaacgctaca ccaattacca gcggcggaac gaagctacca
    20581 atgaagatgc cccagtccca catgttacgc cagcgggttt cttcaatctt ggagcggtag
    20641 tcaaaaccga ccggacggaa gaacaaagac gccagcacga ggatcatcgc cacatagaag
    20701 ccggagaacg cagcggcata gaccatcggc caggcagcaa agagtgcgcc gcccgcggtg
    20761 atcagccaaa cctggttacc gtcccagtgt ggtgcaatgg agttaatcat aattcgacgc
    20821 tcggtgtcgt tacgaccgag gaaacgggtg agcatgccca cccccatgtc gaaaccgtca
    20881 gtgactgcaa aaccaatcag cagaacgcca accagcagcc accagataaa acgcaatact
    20941 tcataatcga tcatttgacg actcctgtct tagcgtgccg gctgagtagt cgtggaagac
    21001 tgctcaaagt gatagcgacc ggttttcagg ctgcttgggc cgaggcgtgc aaacttgaac
    21061 attaagaaca attctgccac caggaacagg gtatacaggc cgcaaatcag caccattgag
    21121 aagatgagat cgcctgcggt cagtgacgaa ttcgccacag ctgtcggcag cacttcaccg
    21181 atagcccacg gttggcggcc atattcagcc acgaaccagc ccgcttctac agcaatccac
    21241 ggcagcggaa taccgtacag cgcggcgcgc agaagccatt ttttctcgcc aatgcggttg
    21301 cggatgacac tccagaaaga gagcgcgatg attgccagca gcaggaagcc acacgccacc
    21361 atgatacgga acgcaaagta cagcggcgct acacgcggga tggagtcttt ggttgcctgt
    21421 tgaatctgcg cttcagtcgc atcagccacg tttggcgtat agcgtttcag cagcagaccg
    21481 taaccgaggt ctttcttcat gctattgaac tggtcacgaa ccgcctggtc ggtagaacca
    21541 gaacgcagtt gttcgagcag agagtacgcc ttcatcccgt tacgaatgcg ttcttcatgc
    21601 tgcaccatca gctctttcag gccgataacc ggggtatcca cggaacgcgt tgcaatgatg
    21661 cccagtgcgt aagggatctg aatcgcaaat ttgttcgtct cctcttcctg atcaggaatg
    21721 ccgaacaaag taaaggcagc aggcgcaggt tgcgtttccc actcggcttc aatagcagcc
    21781 agtttggttt tctgcacgtc gcccatttcg tagccggatt catcacccag aacaataaca
    21841 gacagaacag cagccatacc gaagctggca gcgatagcaa aggagcgttt agcgaaggcg
    21901 aagtcacgac ctttcagcat ataccatgcg ctgataccga ggatgaacat cgcgccagtc
    21961 acataaccag acgctacagt gtgaacgaat ttcacctgag caaccgggtt aagcaccagc
    22021 tcggagaagc tcaccatctc catacgcata gtttcaaagt tgaaatcgga cgcgattggg
    22081 ttttgcatcc agccgttcgc aatcagaatc cacagtgcgg acaggtttga accgagcgcc
    22141 accagccagg tgacacacat atgctgaact ttacccagac gatcccaacc gaagaagaac
    22201 agacctacaa aggtggattc gaggaagaag gccatcagac cttcgattgc cagcggcgca
    22261 ccgaagatat cccctacata gtgggaatag taagaccagt tagtcccgaa ctggaactcc
    22321 atggtcagac cggtagccac acccagagcg aagttgatac caaacaactt gccccagaac
    22381 ttggtcatat ctttataaat ctgtttgccg gagaggacgt agaccgtttc cataatggcc
    22441 aggaggaacg ccataccgag cgtcagtggc acaaaaagga agtggtacat cgcggtcaag
    22501 gcaaactgta agcgcgacag ttcgactata tctaacatca tgactccttg ctcatcgcat
    22561 gaagactccg agagtgaacc ccgttagaaa gggtcacacg catgccccaa tagaaatttg
    22621 tttgaacact ttcatcatac catgtgaatg cacacaaaaa cggcgtaaag aaaggttgca
    22681 aacatgttaa taaaaactca aattgatccc acgtatatat tacgccgcaa aatccttaca
    22741 ataaacaggt ttttgttgaa gcaaatttgc atttttcgac ggtgatcaat ttatagctaa
    22801 attgccgcct ttcagccaat ttgatcgaga acaatttatc tctttttgat gcccatttcc
    22861 aagacttata cattgataaa tatcaatttc tacaaattat gttaatcatg tttcaacaag
    22921 gtaactttag tgacatttat gtttaaaatg tgtgaattat aagactgaaa ttagtttcgg
    22981 ttggcaatta aagataaaaa gagacgaagc aaaaacagca cggttgacct taattctttt
    23041 tttggtcact ggctaatacg acgcagagtt tcaggattat taataaaggc ataaatctcc
    23101 tccccgccga tattaacatt taattaactt ataattatat tataattaac tcacccgggg
    23161 ttattatttt gttaaaaata aagccacata tacgtagctt gagagtgaag ataaacgcaa
    23221 aattacctga tgcgctacgc ttgccagtgt ctacatcatc tctgcaatat attgaatttg
    23281 cctgcttttg tagaccgaat aaagcgttca tgccgtatcc ggcatgaaca aagcgccctt
    23341 tgtcaacaat ctggccgcgc atatgtggct tcggtttgat tgctattcag gcaagccggt
    23401 aactgaacgt ccgtgactgg ccgggtaaaa aaggccccga tagatttgaa ccttgatttt
    23461 cctcggtggt aatgtgttca tccatcatcg tttctgagca agaaatcacc tcgcgactga
    23521 aagcaacagt cgcatcacag gttgctgatt cagccggatt aaacagccgt aaaatcactt
    23581 cttgtcggtc ttcagctttc ttaagtgcgc ttatcaggca tcccactggg ggcattttca
    23641 acaaactata actttccggc acgttgaatc cggctttgtt gagcttcatc gcatcccatg
    23701 ggattttgtt gtaacactgt actggagtca gccatgctcg cgcctgctga gctacaccag
    23761 cggcggttgg cgtaccggta taactcaata aacttaggcg acaagaaagc agaccacgta
    23821 gttgtgagtc cgggactggc attttaattc ccgaaggccg cctaggcctt aaaagcagat
    23881 cttctttccc cagtaagccc acgccgcgca gcaacgtaat ggcaaaggtt ttcttctctt
    23941 caccgatgac ttcaaattca cgtagccctt cgctaaagac agccatgccg ttacgccctt
    24001 cctgtaaggc tgcatagttg agcatattcc ataccggaac cggcgcttct ttccagcctt
    24061 cttgctgcca gttgttcatt gcactgtcgt tcacggggcg cgttagcgaa ccaaactggg
    24121 tatctgccag aacactatcg gtgttaaaag atgtagggat caggacgcga aggcgatgat
    24181 cgtcagcctg gttatcaagg ttgatatcca catcaatgcg cctgttgtta tgactaagag
    24241 tgacaaccaa ctcgacacct actctgccag tggattgtct tgcgctgcgt tgttgcagat
    24301 tacgcggcac cgccatttca tagcggataa cagccctgct ctgccaggcc tcatgaataa
    24361 tctcgcattg cggtttcgca ttcgccgaag taattaccca ctcttctttt gcgggtgaat
    24421 agtcatattc atcaccatca tcagagcttt cttcaatttg caacacccgg tcatagcgca
    24481 caccgctatc tttatctacc agttgtagcg ttccatcctc attgagcgca atttgccaga
    24541 aagcattttc cagtatccct tcagcgtcac tttttgccgc aatcacgttg ccaggctgat
    24601 tcgcttcgat ataaagcgtg cgatagccca tagaagggac aatctggttg atctgtatat
    24661 caaactccat aaagggatcg taattaccgt aatgaactat ttgccgatcg attaggcctg
    24721 gatcgatctc acgggcatgg cgaataaaat acggtacagg ctgaccgcga tcgtctcgta
    24781 gattaaactg gctggcgcgc agccgcacag tggtgttgat cacttcttcc cgcggccagg
    24841 gcatcaggtt aaacaggacg agtttgtcgg cgtcgctctg cggcatgttg tcggcgattt
    24901 tacgcatata gaaacgcagc agattatccg ccatgtcttc agccagttcg aagcgggcga
    24961 cgatttcgcg atgaactttg tcactacagc agcagccgat gctgtcgtgg gcatgatttt
    25021 ttaagatctc tttccatatt ttctccagca agccgtggtg gtattcaaaa cccaacgtcc
    25081 aggccagcgt tgccagcggt tccagcggtt ccagcggttc cagcggttcc agcggttcca
    25141 gcggttccag cagattaaca atcttatttt caatacgtgc gtgggcaact ttgatatcca
    25201 tgcgcgtgga accgatggtg cgatgcacgc gcatatattt gccatcaata aattcccctt
    25261 tcagggttgc cagattatct cgctgcgctt cgatcttctc aaatacctct tcaaagcggc
    25321 tcatcacaaa ctttcgttga ggataaattt cgcgcagttt ctccatcact tcgaagatat
    25381 tttgctgcaa tggcatctga tcatgcccat tcggcagcaa aatctctttg gttacagacg
    25441 ctttttccag cacgtcaaaa taactgtcga ggcgtttgcg gaatccgttt tcgtccgcag
    25501 gtaagtactt accgatggca taacccaacg gcatcacctg cgccgtcact tcgctaccgt
    25561 cactgctttg ccacaaaaac tcggttttat ccgtaccgtg gcgctcagaa catccacgcc
    25621 agaacatggt gcaggtaatg ccaaatccat tgtagatatg cggcagttgc ccggacatgc
    25681 caaaggaatc gggtaaataa cctattttca tcggctcgcc aaacgcgagg cagtcacgca
    25741 ttccgtacat cagattacgg acgatggatt ccgcagaaac aatcgtggta tcggtctggg
    25801 tataccaggg gccgataatc aacttgccgg cttctaccag tttcttcacg cggtctttgt
    25861 tttccggttt caccgcgaaa taatcttcga ggatcgccgt ttgcccgtcg agtacgtaat
    25921 atttgtattc gttgtcctgt tccagtcggc acaggatctc ttccatatta ttgaccagca
    25981 gaatgcgtga ctcttcggtg gtgaaatacc actcccgatc ccagtgcata tgcggggtga
    26041 tgtgaacgcg agataccgct ttcatcgtcg tttccttttt gtttattgca ttacgccatc
    26101 agtcagatag ttgccatgct taaccgcgtg acgccgccag atcaggagaa ttgcagtcga
    26161 gattgcagcc cccaccaatg ccgcaccaaa ccagccaatt gccgccataa caccgcccgc
    26221 gccattatca tgaagtaaaa agagcgagaa aatgccagca ccgggtgtcg aaaggccgat
    26281 attcatcgca ccgacaatag cgcccgttac catagagccc agcacaaacg aaccaataac
    26341 ccgcagcgga tcttcaatcg ccatcgggat cgccccttcg gtaatacctg ccagccctaa
    26401 cagccaggtg gatttcccgg tctcaatttc aaactcttta aacaggcgcg gtgcgagcat
    26461 cgtggaagcg gttacggtaa atgccgaaac cattttgacg gaggcgaaaa tggcatacgg
    26521 gccgtaaacg ccgttcgcca ttgcgcccag gcagaatgca taagcggctt tattcactgg
    26581 ccctccaagg tcaaaggaac acataaaacc gagaatggca cccagcaaca gcgcgttact
    26641 tcctgacaga gcgttcagcc aggcggtaag cgagttattg atccaggcga caggttcccc
    26701 caccacaaac agcatcagac tgcccgctcc caatgtaccg agcaccaggt agagataaaa
    26761 agtcaggaag ccattgaatt tactgctaag acgcaagtga tttttcatcc agcgtatcaa
    26821 gtaaccggct atcaatccgc caacgaccgc gccgagaaac ccggagccga tcatgttggc
    26881 ggcaagtcca gccgcaaagc ctggcgctaa cgccggttta tctgccagcg aataggcggt
    26941 ataggctgcc agcaccggta ccatcaaaat tccgagcagc ccgccgccca gtttgcggta
    27001 catccacagc caggagtttt cttcattaaa cagatcttgt agcccgaaga tttgcgacag
    27061 taataccgcg accgccagca ccgtcccccc cgcgacaatc aacggtacgg caaaagagat
    27121 cccgctcaac agtgcctgtt tcagctccgt tttgacgctt ttcaccggtt gcgtatcttg
    27181 ctgtacggta cgcgtctcat cgccacgctc gagggtaagc gattgttgga tcaacgcttc
    27241 tgcatggcga atcggctcgg caacaggcac agaaagcgca ggaatgccgt taaaacgctc
    27301 actctccttg atggcgactt cagccgcaaa aatacaggca gttgcactat tgagttgatc
    27361 cgccgttaaa cgcccttcaa taccgttagc accttgcttt tcaacgtaga cattcacgcc
    27421 gagtttgcgt ccggcttttt ccagatactc cgcagccatg taggtgtgag caatacccgc
    27481 cgggcaggcc gttacgcaaa cgatagttgg cgcgttggaa aaagaggcag aaggttgcgt
    27541 gcctcccttg tcatccagcg ccgagagcaa ttcatcaggc gtcgtcgccg actgtatacg
    27601 cgcccgaatc tcatcatccg ccaggcgcgt ggtcagcgct gtcagcagtt gcatgtgcgt
    27661 agtacccgct tcattggggg gaatcgccag caacaccact aaatcaaccg cttccgggcc
    27721 atccacgctt tcccactgaa gcggctcgct gagtgtggcg accgcaaacg ccgcttcttt
    27781 taccgcagca gttttgccat gcggcacagc caacccttca cctaaggcgg tcgggccaag
    27841 gctttcacgg cgatacactt ctttcagaaa ttgctcagta ctggaaattt tccccagagc
    27901 agcaagacgt tgagtcaacg cgtgaatggc ctcttcacgg ctggtaaagc gcgcattcag
    27961 acacaacgca tcgcggtggg tcagagtcgt caggttcatc atgatcctca cttgatcgtt
    28021 tttatagtcg cgccagtgtg cccgataaaa caataccatt gcgtaatcaa tttcacttga
    28081 ttgaaattat ttgcattgta tttgtattat ttacaggatg aatttatcgg gcataatggc
    28141 taaattcgta ttaatgagat gctaaataat gggacacaaa cccttatacc gacagattgc
    28201 tgatcgcatt cgtgagcaaa ttgcgcgtgg cgagcttaaa cctggcgatg cgttacctac
    28261 cgaatcggca ttacaaacag agtttggcgt cagccgggtt acggtgcgtc aggctttgcg
    28321 ccagttagtc gagcaacaga tcctcgaaag cattcagggc agcgggactt acgtcaaaga
    28381 agagcgggtc aattacgata tttttcagtt aaccagtttt gatgaaaaac tgtcggaccg
    28441 tcacgtcgat acacacagtg aagttctgat atttgaagtg attccggctg acgattttct
    28501 tcagcaacag ctacaaatca ccgtgcagga tcgtgtgtgg catgtgaagc gcgtgcgcta
    28561 tcgcaagcaa aagccaatgg cgctggaaga aacctggatg ccgcttgcgt tgttcccgga
    28621 tctcacctgg caggtcatgg aaaattcgaa atatcacttt attgaagaag tgaagaagat
    28681 ggttatcgat cgtagcgaac aggaaatcat tcctctgatg ccaaccgaag agatgagccg
    28741 tctgcttaat atcagccaga caaagccgat tcttgaaaaa gtatcgcgtg gatatttagt
    28801 tgatggtcgg gtgtttgaat atagccgcaa cgcttttaat accgatgact ataaatttac
    28861 cctgatagcg cagcgaaaat catcgcgata agcacaaaaa aggccatcat acgatggcca
    28921 accatgtcga aaccgacagc ccttcgccat tcctgtagag ggaagcggcg agggctattt
    28981 cttattacag atatttattt cagaacagtt ttcagtgctt caccgatatc cgccaggctg
    29041 cgaacggttt tcacgcctgc ggcttccaga gcagcgaatt tctcatccgc agtccctttc
    29101 ccaccggcaa tgatcgcacc cgcgtggccc atacgtttgc ctttcggcgc agtcacacca
    29161 gcgatgtaac ccacaactgg cttggtaaca tgctctttga tgtacgctgc cgcttcttct
    29221 tcagcgctac cgccgatctc accgatcatc acgatcgctt cggtctgcgg atctttttcg
    29281 aacatttcga gaatgtcgat aaagttagag ccagggatcg ggtcaccgcc gataccgaca
    29341 caggtcgact gaccgaaacc gtaatccgtg gtctgtttaa ccgcttcata ggtcagtgta
    29401 ccggaacggg aaacgatacc cactttaccc ggtttgtgaa tgtgacccgg ctggataccg
    29461 attttgcatt cacccggggt gataacgcct gggcagttcg ggccgatcat acgaacgcct
    29521 gcttcgtcca gtttcacttt cacggtcagc atatccagcg tcgggatgcc ttcagtgatg
    29581 gtgataatca gtttgatgcc tgcatcgata gcttccagaa tggagtcttt gcagaacggt
    29641 gctggtacgt aaataacaga cgcggaagcg ccagtagcag caacagcttc acgcacggtg
    29701 ttgaacaccg gcaggccgag gtgggtagtg ccgcctttac ctggagttac gccaccaacc
    29761 attttagtgc cgtatgcaat ggcctgttct gagtggaaag tcccctggct accggtaaag
    29821 ccctggcaga taaccttggt gtttttatca attaaaatgg acattatttc ccctccactg
    29881 cggcaacaac ctgctgagct gcatccgtca gaccttttgc tgcaataata ttcaggccgc
    29941 tgtcagccag tttcttcgcg ccgagttcgg cgttgttacc ttccagacgt accacgaccg
    30001 gtacgttaac acccacttcc gctaccgcgc cgatgatacc gtcagcgatc aggtcgcaac
    30061 gaactatacc gccgaagatg ttaaccagaa cggctttcac tttgtcgtca gagaggatga
    30121 ttttgaacgc ttcggttaca cgttctttgg ttgcgccgcc gccaacgtcc aggaagttag
    30181 ccggttcgcc gccgtgcagt ttaactatgt ccatcgtacc catcgccaga cctgcgccgt
    30241 taaccataca accgatgtta ccgtccagcg caacgtagtt cagttcccac tgtgcagcct
    30301 gtgcttcacg cggatcttcc tgcgactggt cacgcatttc gcgcagatca ggctggcgga
    30361 acagggcgtt gccatcagcg cccagtttgc cgtcgaggca aatcagatcg ccctgtttgg
    30421 tgatgaccag cgggttgatt tcgatcagcg ccaggtcgcg ctccaggaaa atggtcgcca
    30481 ggcccatgaa gattttggtg aactgctgaa ccagtttacc ttccagaccc agtttgaacg
    30541 ccagctcgcg tccctgatac ggcatcgggc cagtcagcgg atcaagcgca actttatgga
    30601 tcaggtgcgg agtttcttcc gccacttttt cgatttccac gccgccttcg gtggaggcca
    30661 taaagaccac gcgacgggaa ctacggtcta caacggcacc gagatacagc tctttagcga
    30721 tatcggtcgc cgcttcaacc agaatctggt taaccggttg gccattggca tctgtttgat
    30781 acgttaccag acgcttgccc agccagtttt ctgcaaaagc acggatgtct tctttgctgt
    30841 ttacaacttt cacaccgccc gctttaccgc ggccaccagc gtgaacctga catttcacta
    30901 cccacggacc ggcaccgatt tttgaagcgg cttcttctgc ttcgcgcgga gtagtacagg
    30961 cataacccac cggtgctggt aagccatagc gggcaaacag ttgttttgcc tgatattcat
    31021 gtaagttcat gtgttctgtc catccttcag taatcgttat cttttaaacc gtagaccgac
    31081 accctatcca cgtcggtcta cagtgcaggt gaagcttaaa ctactacacg tccagcagca
    31141 gacgcgtcgg ctcttccagc aactctttga tcgttaccag gaagcccacg gattcgcgac
    31201 catcgatcag acggtgatcg taggacagcg ccaggtacat catcggcagg atctcaacct
    31261 gaccattcac cgccatcgga cgatctttga tagcgtgcat acccagaatt gcgctctgcg
    31321 gcgggttgat gatcggcgta gacatcaggg aaccgaacac accaccgttg gtgatggtga
    31381 agttaccacc ggtcagatct tcaaccgtca gcttgccatc acggcctttg actgccagct
    31441 ctttgatttt cttctcgatg tctgccatgc cgagggtatc gacatcacgc agaaccggcg
    31501 tcaccaggcc gcgcggcgta gaaaccgcca tgctgacgtc gaaatagttg tggtaaacca
    31561 cgtcatcgcc gtcgatagac gcgttcactt ccgggtaacg tttcagggct tcaaccaccg
    31621 ctttcacgta gaaggacata aagcccagac ggatgccgtg gcgtttttca aacgcttcac
    31681 cgtactgctt acgcagatcc ataatcggct tcatgttgac ttcgttgaac gtggtcagca
    31741 tggcggtgga gtttttcgct tccagcagac gctctgccac acgcttacgc aggcgagtca
    31801 tcgggacacg tttttcacta cgtgcagcca gagccggttg cgccgccgga gcagccgctg
    31861 ccggagcaga ctctttcgcc ggggctttcg ccagatgttt ttccacatct tcacgagtca
    31921 gacgaccacc cacaccggtg cctttaatgg cgctggcgtc gagattgtgt tcagccagca
    31981 ggcgacggat cgccgggctt aacgcatcgt tgttttgctc ttccagagac gcctgctggc
    32041 gttgcgccgg agtggacgct ttctcttcag atttggcgct ggtttcttta ccggcgctgt
    32101 tgccttcacg caggcgacca aggatctgac gagacgttac cgttgtacct tcatcttcca
    32161 gaaccgcatc cagaatgccg tctgctgatg ccggtacttc cagtaccact ttgtcagttt
    32221 cgatttctac cagcacttca tcacgtacga ctgcgtcgcc gggtttttta tgccaggttg
    32281 cgacggtggc atcggctacg gattcaggca ggtcagggac cagaatatct acgctactca
    32341 ttgtgtatcc tttatttatt cgacgttcag cgcgtcatta accagatctt gttgctgttt
    32401 ctggtgaacg gacatatacc ctaccgccgg agaggcggag gccgggcggc ctgcataacg
    32461 cagagaagcc ccaaacggaa tcacttcacg gaaatgatgc tggctgcagt accatgcgcc
    32521 ctggttgagc ggctcttcct ggcaccagac aaaatccttg acgtgagcaa actgctgcaa
    32581 cacttcctgc atcgctttat gcgggaacgg gtagagttgc tcgatacgca caatggcgac
    32641 atcgtgttga ttgttcttac gacgctgttc cagcaggtcg taataaacct taccagaaca
    32701 catcactacg cgcttcacgc ccttcggatc aagttcgtcg atttcaccga tggctggcag
    32761 gaaggtgccg ttcgccagtt cttcgaggct ggaaaccgcc agcggatgac gcagcaggga
    32821 tttcggcgac atcacgacca gcggacgacg catcccgcgc agcgcctgac gacgcagcat
    32881 gtggtaaacc tgtgccgggg tagacggtac gcaaacctgc atgttttgct cagcacaaag
    32941 ttgcagataa cgttccagac gcgcggagga gtgctccggc ccctgccctt cgtaaccgtg
    33001 cggcagcaac atcaccagac cacacatccg gccccatttc tgctcgccag aggagatgaa
    33061 ctggtcgata accacctgcg caccgttggc gaagtcaccg aactgcgctt cccagatggt
    33121 cagggtgcgt ggttctgcgg tggcataacc atattcaaac gccagcacgg cttcttctga
    33181 cagtacggag tcccagacac ggaacgcgcc ctgaccgtta tggatgtgtt gcagcggcgt
    33241 gtaagtggaa ccgttagact ggttgtggat caccgcgtgg cggtggaaga aggtaccgcg
    33301 accggagtct tcacccgaca ggcgaaccgg aatgccttca tcaaccagcg tggcgtaagc
    33361 gaggttttcc gcaccgcccc agtcgaacag tttctcaccg gctgccatcg cctggcgatc
    33421 gccataaatc ttggcaacgc gagactgcat ttcaactgct tctggtaccg tactgatacg
    33481 tttagccagt tcctgcaggc gcttcatctc aactttgttc gggtactctt cgtcccattc
    33541 gtggttgagg tacggcgacc aggtgaaaga gtgcatgttc atcggacgcc actctgccac
    33601 tacgcaatcg ccagcatcca gcgcatcgcg gtacaggtta accatctcgg tggcatcttc
    33661 cagcgtcgcc actttttcct gctccagctt gtcagcgtag attttgcgcg gtgtcggatg
    33721 ttttttgatt ttctgataca tcagtggctg ggttgcgctc ggctcgtcgg cttcgttgtg
    33781 gccgtgacgg cggtagcata ccaggtcgat gaagacatca cgtttaaagg tgttacggaa
    33841 atcgagcgcc agacgggtca caaaggcaac ggcttccgga tcatccgcgt taacgtggaa
    33901 aatcggtgcc tgaaccatct taccgatatc agtacagtac ggtgtagaac gggcatccag
    33961 cgggttagag gtggtgaaac caacctggtt gttgataacg atacgtaccg taccgccaac
    34021 ttcataacca cgcgctttcg acatgttcag agtttcctga accacgccct gcccggtcac
    34081 tgcggcgtca ccgtggatgg tgattggcag cactttgttg ctgctcggct catcaagtct
    34141 gtccagacgg gcacgaacag agccgataac gaccgggctt acaatctcaa ggtgagacgg
    34201 gttaaacgcc agcgccaggt gcaccaggcc gccatcggtc tggaagtcag acgagaagcc
    34261 catgtggtat ttcacgtcgc ctgtgccgag gtgttcttta tgtttaccgg cgaactcgtc
    34321 gaacaagtct tgcggttttt tacccagcac gttcaccagc acgttcagac gaccacggtg
    34381 cgccatcccg agaaccactt cgcgggtgcc gctgttgcca gcgtggcgga tcatctcttt
    34441 aagcatcggg attaacgcgt caccgccttc cagcgagaag cgttttgcgc cagggaattt
    34501 tgcgccgagg taacgctcaa ggccttcagc ggcggtcagt tcgcttaaga agcgtttttt
    34561 ctcttcgcta ttgaaagtcg cgcgaccaga ctcgatacgc tgttggatcc agcgtttttc
    34621 ttcggtgctg gtgatgtgca tatactcggc accaatcggg ccgcagtagg tttgcttgag
    34681 ggcggaaata agctcgccga gtttcatggt ttctttgccg ctggcaaatg aaccgacgtt
    34741 gaaggtttcc tggaagtctg cttcggtcag atcgtggaaa gacggatcca gatcggccac
    34801 tttatcttgc tgccacagtc ccagcggatc tagattcgca tgctggtgac cacggaagcg
    34861 gtatgcgtta atgagctgca ggactttaac ctgcttcaca ttggtgtcag ggtcggagat
    34921 cgttgaagag taacgtgaag cgtctttcgc caggcggcgg aaatattcac gcgtttgaga
    34981 gtggaattga tccggtttga ctcccgtacc aggtaactgc tggaacgtcg aacgccagtt
    35041 agcgtcaacc gagtcaggat cggttaagaa gtcttcatag agctgttcta tccagctctg
    35101 gtttgcgcca gagaggtaag aagagtccaa ccaggctttc aaagcgctgt tctgcatcgt
    35161 gatcccttaa gcatcttttt tatgcttact tcgccgtgga tactaccacg cacagtgatg
    35221 tgcgtgccgg ggttcacttg cgagctcttg tctacgtagt ggctcgcgaa ggaaccttta
    35281 aaaactgtct tagtgtcagg ggcagttttt aaagatttcc tgcatttctt tgttacgcct
    35341 gatgcgctgc gcttatcagg cctgcgggta atgacttgta ggccggataa ggcgttcacg
    35401 ccgcatccgg cactggttgc ctgatgcaac gcttgcgcgt cttatcaggc ctacagttta
    35461 cgcattacgt tgcaacaaca tcgacttgat atggccgatg gcgcgcgtcg ggttcagccc
    35521 cttcggacat acactgacgc agttcatgat gctgtgacag cggaatacgc tgaatgcatc
    35581 gctcaaaccg tcgaggcggc tgtcagtctc ggtatcacgg ctatcgatca ggaaacgata
    35641 tgccgctaac aagcctgccg ggccgataaa cttatcggga ttccaccaga aagacggaca
    35701 agaggttgaa caacatgcgc agagaataca ttcatacagc ccgtcgagtt tttcgcgctg
    35761 ctctggcatc tgtaaatgct cgcgagctgg cggattttgt ccattattca acaggtaagg
    35821 cttaattttc tcatattgcg catagaattg tcccatgtct accaccaaat cgcggatcac
    35881 cggtaaacct ggcagcgggc gaatcacaat cttcttgccc ggctggttga gtgccgaaat
    35941 cggggtaata caggccagac cattcttgcc gttcatgttc agaccgtcgg aaccgcacac
    36001 accttcacgg caggagcggc ggaacgacag gctgggatct ttctctttca gctggataag
    36061 cgcatccagc agcatcatgt cgcgaccttc ttccgcttcc agggtgtaat cctgcatacg
    36121 cggagcatca tcaacatccg ggttatagcg ataaattgaa aactcgagtc tcattttcct
    36181 gtctccgcat tagtaagtac gaatcttcgg cgggaatgcc gggcgcagtt tcggttccat
    36241 gttgacgctt cggcgcgtca tggattccga ctcaggcaga tacagggagt ggcacagcca
    36301 gttttcatca tcacgatccg ggaagtcgaa gcggctatgt gcgccacggc tttcggtacg
    36361 gaagttggca gaaacagccg ttgcatacgc cgtttccatc aggttatcca gttccaggca
    36421 ctcaacgcgc tgggtgttga actcgctgga agtgtcatcc agacgggcat ttttcagacg
    36481 ctcgcggatc actttcaact gctcaagccc tttcgccatc gcatcacctt cacggaagac
    36541 cgagaagtta tgctgcatac attcttgcag cgctttacgg atcgccaccg gatcttcacc
    36601 gttacgatta ttgttccacc ggttcaggcg atccagagac gcttcaacat cagactcgct
    36661 ggcatcgcgc agtgcgccct gctcggcgat agactcttgc agatgcagac ctgccgcgcg
    36721 accaaagacc accaggtcca gcagcgagtt gccgcccaga cggttagcgc cgtgtaccga
    36781 tacacaagcg atttcaccaa cggcaaacag tcccggaaca accacatctt cgcctttctc
    36841 attcacagtc agcgcctgac cggtaacttt ggtcggaata ccgcccatca tgtagtgaca
    36901 ggttgggata accggaatcg gctctttcac cggatcgacg tgagcgaagg tacgggaaag
    36961 ctccaggata cccggcagac gggattcgag aacttcttta cccaggtgat cgagtttcag
    37021 tttcgcgtgt ggcccccacg gaccatcaca gccgcgacct tcacggattt caatcatgat
    37081 ggaacgcgcc accacatcac ggcccgccag gtctttggcg ttcggcgcat aacgctccat
    37141 aaagcgttcg ccatgtttgt tcagcagata accgccttca ccacggcaac cttcggtgac
    37201 cagtacgccc gcaccggcaa tgccggtcgg gtggaattgc cacatttcca tatcctgcac
    37261 cggtacgcct gcgcggatag ccatgccgac accgtcgcca gtgttaatgt gggcgttagt
    37321 ggtggactga taaatacgcc ctgccccgcc agtcgccagc acggtagcgc gggctttgaa
    37381 ataaaccact tcaccggttt cgatgcacag tgcggtacaa cccaccaccg cgccatcctg
    37441 gtttttcacc agatccagcg cataccactc ggagaaaatg gtggtgtggt ttttcaggtt
    37501 ctgctgataa agcgtgtgca acagtgcgtg accggtacgg tcagcagccg ccgcagtgcg
    37561 tgccgcctgc tcgccgccga agtttttcga ctgaccgcca aacggacgtt gatagatacg
    37621 accatcatcg agacgcgaga acggcaggcc catgtgttcg agttccagaa tcgcttccgg
    37681 cccggtttta cacatatatt caatcgcgtc ctggtcaccg atatagtccg acccttttac
    37741 ggtgtcgtac atatgccatt cccagttatc ttcatgggta ttacccagcg caacggtaat
    37801 gccgccttgc gcagaaacgg tatgggaacg ggtcgggaag actttagaga gcagcgcaca
    37861 ggtctggccg ctctgggaaa tttgcagcgc cgcgcgcata cctgcgccac cggcaccaat
    37921 cacaactgca tcaaattctc tgactggcaa tttcatcaca ccccccacac cacaacgaat
    37981 ccataaatca cgtaaaccac cagtgcaacg acaatcacca gttgcagcat caggcgcaag
    38041 gccagcggtt taacgtagtc ggtcaacacc tgccacatgc cgatccaggc atgaatcaag
    38101 atagaaaaca gcgccagcag ggtgaacact ttggtgaacg cagaggcgaa gaaaccgatc
    38161 cagacttcat atgtcagttc gccactggtg gcgaaaaaac cgaccatata aatgatgtag
    38221 agcgtcagga cgatagcggt agcgcgaacg aggatgaaat catgtacgcc attgcgtcct
    38281 aatgcggagg cgttgcttac catacgagga ctcctgcgag aagtgaaagc acgacagtaa
    38341 taacaaagga gattttggcg gagcgtttac ccgcttcgaa tgtttcttcc agatagccaa
    38401 aatccatcat catgtggcga atacctacga cgacgtgata cgccagagcg gtaaggatgc
    38461 cccacatgat aaatttgacg aagaagctgc ccataatcgc ggaagcttgc tcgaaacctt
    38521 caggggaaga gaggctggta cccagaagcc acagcaggat gcccactgca acaaaggtga
    38581 tcacaccgga aacgcgatgg agaatggacg ctatcgccgt gatggggaac cggatggtct
    38641 gtaggtccag attaacaggt ctttgttttt tcacatttct tatcatgaat aacgcccaca
    38701 tgctgttctt attattccct ggggactacg ggcacagagg ttaactttct gttacctgga
    38761 gacgtcggga tttccttcct ccggtctgct tgcgggtcag acagcgtcct ttccataact
    38821 gcgcgtcatg caaaacactg cttccagatg cgaaaacgac acgttacaac gctgggtggc
    38881 tcgggattgc agggtgttcc ggagacctgg cggtagtata ggctgttcac aaaatcatta
    38941 caattaacct acatatagtt tgtcgggttt tatcctgaac agtgatccag gtcacgataa
    39001 caacatttat ttaattttta atcatctaat ttgacaatca ttcaacaaag ttgttacaaa
    39061 cattaccagg aaaagcatat aatgcgtaaa agttatgaag tcggtatttc acctaagatt
    39121 aacttatgta acagtgtgga agtattgacc aattcattcg ggacagttat tagtggtaga
    39181 caagtttaat aattcggatt gctaagtact tgattcgcca tttattcgtc atcaatggat
    39241 tctttacctg caagcgccca gagctctgta cccaggtttt cccctctttc acagagcggc
    39301 gagccaaata acaaacgggt aaagccaggt tgatgtgcga aggcaaattt aagttccggc
    39361 agtcttacgc aataaggcgc taaggagacc ttaaatggct gatacaaaag caaaactcac
    39421 cctcaacggg gacacagctg ttgaactgga tgtgctgaaa ggcacgctgg gtcaagatgt
    39481 tattgatatc cgtactctcg gttcaaaagg tgtgttcacc tttgacccag gcttcacttc
    39541 aaccgcatcc tgcgaatcta aaattacttt tattgatggt gatgaaggta ttttgctgca
    39601 ccgcggtttc ccgatcgatc agctggcgac cgattctaac tacctagaag tttgttacat
    39661 cctgctgaat ggtgaaaaac cgactcagga acagtatgac gaatttaaaa ctacggtgac
    39721 ccgtcatacc atgatccacg agcagattac ccgtctgttc cacgctttcc gtcgcgactc
    39781 acatccaatg gcagtcatgt gtggtattac cggcgcgctg gcggcgttct atcacgactc
    39841 gctggatgtt aacaatcctc gtcatcgtga aattgccgcg ttccgcctgc tgtcgaaaat
    39901 gccgaccatg gccgcgatgt gttacaagta ttccattggt cagccatttg tttatccgcg
    39961 caacgatctc tcctatgccg gtaacttcct gaatatgatg ttctccacgc cgtgcgaacc
    40021 gtatgaagtt aatccgattc tggaacgtgc tatggaccgt attctgatcc tgcacgctga
    40081 ccatgaacag aacgcctcta cctccaccgt gcgtaccgct ggctcttcgg gtgcgaaccc
    40141 gtttgcctgt atcgcagcag gtattgcttc actgtgggga cctgcgcacg gtggtgctaa
    40201 cgaagcggcg ctgaaaatgc tggaagaaat tagctccgtt aaacacattc cggaatttgt
    40261 tcgtcgtgcg aaagataaaa atgattcttt ccgcctgatg ggcttcggtc accgcgtgta
    40321 caaaaattac gacccgcgcg ccaccgtaat gcgtgaaacc tgccatgaag ttctgaaaga
    40381 gctgggcacc aaagatgacc tgctggaagt ggctatggag ctggaaaaca tcgcgctgaa
    40441 cgacccgtac tttatcgaga agaaactgta cccgaacgtc gatttctact ctggtatcat
    40501 cctgaaagcg atgggtattc cgtcttccat gttcaccgtc attttcgcaa tggcacgtac
    40561 cgttggctgg atcgcccact ggagcgaaat gcacagttac ggtatgaaga ttgcccgtcc
    40621 gcgtcagctg tatacaggat atgaaaaacg cgactttaaa agcgatatca agcgttaatg
    40681 gttgattgct aagttgtaaa tattttaacc cgccgttcaa atggcgggtt aatttttata
    40741 tgcctaaaca caaaaaattg taaaaataaa atccactatc ggacattcat atattctaaa
    40801 aaaaaatata tcggtataaa ttataaacaa atcgatgatt tttaatcatc tatcttcctc
    40861 tcagttacat gaataaacca ccaaatatag cgcaccccta taatttccaa tactttattc
    40921 atcaaacatt taattcatac catcaattaa ttacttttac atttaataaa attaataata
    40981 tttttaagaa aaactaaaaa cattcgacaa tcaaataaga tattcttgca ccgatatact
    41041 tttttctttt ctgttaaagg actcagttat gtttaaagga caaaaaacat tggccgcact
    41101 ggccgtatct ctgctgttca cagcacctgt ttatgcggct gatgaaggat ccggtgaaat
    41161 tcactttaaa ggtgaagtta ttgaagcacc gtgtgaaata catcaggatg atattgataa
    41221 agaggttgaa ctcggtcagg tgaccaccag ccacattaat cagtcacatc acagcgatgc
    41281 cgttgctgtc gacctgctct tagtcaactg tgatctggaa aactccagca acggttccgg
    41341 tggcaagatt tctaaagttg cagtcacctt tgatagctca gcgaaaacca ccggcgcaga
    41401 tccgattctc aacaacacca gcacgggtga agccaccggc gtcggcgtac gtttaatgaa
    41461 taaagatcaa agcaatatcg ttttaggcac cgctactcca gatatcgacc tggctccgac
    41521 ctccagcgaa caaacgctga atttctttgc ctggatggaa caaattgatc aggctacacc
    41581 tgtaacgcca ggcgcagtta cggcgaatgc aacctacgtg ctggattata aataagtatt
    41641 actttggcgg cgctatcgcc gcctttcata aaatatttat caggtatgga caccgtgaat
    41701 atttatcgac tctcttttgt atcctgcctg gtcgtggcga tgccttgcgc attggcggtc
    41761 gaattcaatc tgaatgttct cgataaatca atgcgcgacc gcattgatat ttcattatta
    41821 aaggaaaaag gagtcattgc tcccggtgag tattttgtta gcgttgcggt aaataataac
    41881 caaatcagta acgagcaaaa gattaactgg cacaaaaatg acgataaaac cattccgtgc
    41941 atcaatgatt tactggtcga taaatttggc ttaaaacctg aagtccgtca gtcattacca
    42001 ttgataaatc agtgcgtcga ttttagctcc cgacctgaaa tgctcttcaa tttcgatcaa
    42061 gccaatcagc aactgaatat tagtattccg caagcctggc tggcgtggta ctcagaaaac
    42121 tggaccccac cctccacatg gaaagaaggt gtcgccggtg tcctgatgga ttacaacttg
    42181 tttgccagca gctaccgccc acaggatggc agcagcagca ctaatcttaa cgcctatgga
    42241 acagccggaa tcaacacggg ggcatggcgc ttacgcagtg attatcagtt gaatcatact
    42301 gatagcgatg ataaccatga acagtcaggc gaaatatcac gtacctatct ttttcgcccg
    42361 ttaccgcaat taggttcaaa attaaccctc ggcgaaacgg attttagttc caatattttc
    42421 gacggttttt cttataccgg cgcggcactg gcaagtgacg atcgaatgtt gccatgggaa
    42481 ctacgcggct acgccccaca aattagcggt attgcacaga ccaatgccac ggtgacgatc
    42541 agtcaatcag gccgcgtcat ttaccagaaa aaagtcccac caggcccatt tatcattgac
    42601 gaccttaatc agtctgttca gggcacactg gatgtcaaag tgacggaaga agatggtcgg
    42661 gtgaacaatt tccaggtttc agcagcatcg acgcccttcc tgactcgtca gggacaggtt
    42721 cgctataaac tggccgcggg tcagccacgg ccctccatgt cacatcaaac tgaaaatgaa
    42781 acctttttta gcaatgaagt ttcctggggg atgctgtcaa acacctcgct gtacagcggc
    42841 ctgctgcttt ctggtgatga ctaccattct gccgcaatgg gtattgggca aaatatgctg
    42901 tggcttggtg cgctgtcgtt tgatgtcacg tgggccagta gccattttga tactcagcag
    42961 gacgagcggg gcttaagcta ccgttttaat tacagcaaac aagtggatgc cactaacagc
    43021 acgatttccc tcgccgctta tcgtttctcc gatcgtcatt ttcacagcta cgccaactat
    43081 ctggatcaca aatacaacga cagcgatgcg caggacgaaa aacagacgat cagcttatct
    43141 gtgggccaac cgattacccc actaaacctc aatctttacg ccaacctgct acatcaaacc
    43201 tggtggaatg cagacgcctc cacgaccgcc aacatcacgg caggttttaa tgttgatatt
    43261 ggtgactgga gagatatctc tatttctacg tcattcaata caacccatta cgaagataaa
    43321 gatcgcgaca accagattta cctgtcgatt tcgctcccct tcggtaacgg tggtcgggtt
    43381 ggctatgaca tgcaaaacag tagccacagc accacacacc gcatgtcgtg gaacgatacg
    43441 ctggatgaac gtaatagctg gggcatgtct gccggactgc aatccgaccg tcctgacaat
    43501 ggagcccagg tgagcggtaa ctatcagcac ctgagttcag cgggtgagtg ggatatttct
    43561 ggtacctatg ccgccaatga ttacagttcc gtcagcagca gctggagcgg ttctttcacc
    43621 gcaacccaat atggtgcagc gtttcatcgc cgcagctcca ccaatgaacc acgcctgatg
    43681 gtcagcaccg atggcgtggc agatattccg gttcagggca atctcgacta caccaaccat
    43741 tttggcattg cggtggtgcc gttgatttcc agttaccagc cttccaccgt ggcggtgaac
    43801 atgaatgact tacccgacgg cgtaacagtt gcagaaaacg ttatcaaaga aacgtggatt
    43861 gaaggcgcga taggttacaa atcactggct tcccgttccg gtaaagacgt taacgtcatc
    43921 attcgcaacg ccagcggtca gtttcctccc ctcggagcgg atatccgcca ggatgacagc
    43981 ggcattagcg tggggatggt tggcgaggaa ggacatgcct ggttaagcgg agtcgctgaa
    44041 aatcaaaagt ttaccgtggt ctggggtgat agccagtatt gctcgctcca tcttcctgaa
    44101 catatggaag acaccgcaaa tcgcctgatt ttaccttgtc attaattaag gaaatgaaaa
    44161 tgacatttat gaaaggacta ccccttttgt tgttggttgc cagtctgtgc agccacgctg
    44221 cactacaacc cgatcgcact cgtattgtgt ttaacgctaa tgataaagct accagtctgc
    44281 gggtggataa tcgtagcgat aaactcccct acctcgctta ttcctggctc gaaaatgaaa
    44341 aaggggagaa aagcgatgat cttctggtcg ccctgccgcc gatacaacgt ctggaaccga
    44401 aagcaacaac gcaggtacgg atagtaaagc aagcctcgac cacaaaatta ccgggcgatc
    44461 gcgaaacgct atttttctac aatatgcgtg aaattcctcc cgccccggaa aaaaacagcg
    44521 accatgccgt tctgcaagtc gccattcaga gtcgtatcaa ggtgttctgg cgaccggctg
    44581 cattacgcaa gaaagcagga gaaaaggtcg aactgcagtt acaggtcagc cagcagggca
    44641 accaactgac actgaaaaat cctaccgcgt attatctgac cattgcctac ctgggacgaa
    44701 atgaaaaagg cgttctcccc ggcttcaaaa ccgtgatggt tgcgcctttt agtacggtca
    44761 acacaaacac cggaaattat agcggcagcc agttttatct cggttatatg gatgattacg
    44821 gcgcgttacg catgacgacg ctcaactgca gcggacaatg ccatttacaa gccgtggagg
    44881 cgaagaaatg agtgctggca agggagtgtt gctcgtcatc tgtctgttat ttctgccgtt
    44941 gaagtctgcg cttgccctga actgctactt tggttcatcc ggtggctcgg tagaaaaatc
    45001 agaagcgatt caaccttttg ctgtaccagg caatgccaaa ctcggcgata agatctggga
    45061 atctgacgat attaaaattc ccgtctattg tgacaacaat accaacggca actttgaaag
    45121 cgaacatgtt tacgcatggg taaaccccta cccgggggtg caggatcgtt attatcaact
    45181 cggagtaact tacaatggcg ttgattatga cgccaaccag ggaaaaagcc gcatcgacac
    45241 taaccagtgt atcgacagta agaacattga catttatacc cctgagcaga tcatcgcgat
    45301 gggatggcag aacaaaattt gctcgggtga tcccgctaat attcacatgt cgcgtacctt
    45361 tctcgctcgc atgcggttat acgtcaaaat acgagaaatg ccgccgcatg attatcaaag
    45421 tacgcttagc gactatatcg tcgtgcaatt tgacggtgcc ggtagcgtta acgaagaccc
    45481 tactgcccaa aacctgaaat atcatattac tggtctggaa aacattcacg tgctggattg
    45541 cagcgtcaat ttttccattt caccggaaac acaggtgatt gattttggta aatttaattt
    45601 gctggatatt cgtcgccaca caatgtcgaa aacattcagt attaagacga caaaatcaca
    45661 aaatgaccaa tgcaccgacg gatttaaagt cagttcctcc ttttataccg aagaaacgtt
    45721 ggttgaagaa gataaagcat tgttgattgg taatggtctg aagctacgtt tgctggatga
    45781 aaacgcctcg ccttatacct tcaataaata tgctgaatac gccgatttca ccagcgacat
    45841 gttggtctac gaaaaaacct atacagctga actttcgtcc attgcaggta cccccatcga
    45901 ggctgggccc ttcgatacag tggtgctttt taaaattaac tataactgat ttgccatgat
    45961 gccctttgca gccggaaagg gcatctcttt cagaaaattg ctgattttcc ccttttcctt
    46021 gattgctgtt attttttcct agcccggctt tctttatgct gtatcgatac accaaagaga
    46081 aaataatgag ggagcgaagg atgccagttt tgcagtgggg aatgttatgt gtgctatcac
    46141 ttctcctttc tattggtttc ctcgcggtac atctcccggc ggcgctatta cttgggccga
    46201 tgatcgccgg gatcatcttc agtatgcgcg gaattactct gcaacttccc cgctccgctt
    46261 ttctcgccgc gcaggccatt cttggctgca tgattgcgca aaacctcact ggttcgattc
    46321 tcaccacgct ggccgccaac tggccgatcg tgcttgctat tttactggtg acgctgcttt
    46381 ccagcgccat cgtgggttgg ttattggtgc gctatagctc actgcctgga aataccggtg
    46441 cctggggttc ttctccaggc ggcgcagcgg caatggtcgc tatggcgcaa gattacggcg
    46501 cagatattcg cctggtggcg tttatgcaat atctgcgagt gctgttcgtc gcgggggccg
    46561 ctgttctggt cacccgcatg atgctgggtg ataacgctga agcggttaac cagcaaatcg
    46621 tctggttccc gccagtgagc attaatctcc tgcttacgat tttgctcgca gtcgttgccg
    46681 gtacggcggg atgcatgctg cgtcttcctt ccggcacgat gctcatcccg atgctggcgg
    46741 gcgcagtgct ccagtctggt cagctcatca ccatcgaact accggaatgg ctgctggcga
    46801 tggcgtatat ggcaattggc tggcggattg gtcttggttt cgataaacaa atcttactgc
    46861 gggcactacg cccgctaccg caaatcctgc tgtcgatttt tgctctgctg gctatttgtg
    46921 caggtatggc gtggggactg acccggttta tgcatattga ttttatgact gcctacctcg
    46981 ccaccagtcc cggcgggctt gataccgtgg cggtcatcgc cgcagggagc aatgccgata
    47041 tggcgcttat tatggcgatg caaaccctgc gcctgttcag tattttgctg acagggccag
    47101 ccattgcgcg gtttatttca acctatgcgc cgaagcggtc ggcctagtgc tggcagcctg
    47161 gacaccagta aaacgggcgc gaggacaacg tggttttctc aatgatgccg ccacagcgtt
    47221 cgcacagttc gccatcacga tgaaaaacct taaagcgaaa cagcgcccca tgatgcttat
    47281 tctcatccac ctgcccccgc gtagcgtaag aaaaccgagg aatctccagt aacgcgtgtg
    47341 cgagtgcatc cagttgcgcc gcattgagat ctttcgcttt atgatttcca gtcaacccaa
    47401 cctgccagag gatctccacc cgcaaataat tgccaagccc agccagaaac gcctgatcga
    47461 gcagtaatcc tgcaaactga cggttacgaa agcgcggcga caataaccgt tcctttacca
    47521 cctccggcgt cagattcgga tccagcacat cgggaccaac gcgttgtaaa aacggatgcg
    47581 tggtcagttg ttccggcctc aacatctcaa tatccgaggc gctataaagc agaatggttt
    47641 tgtcagccgt ttgcagtttt acccgcaata ctcgcgtggt ctgcggctct tcgccggtat
    47701 caaccacgcg ccagacgccg taaagctgat tatggctgta gagcgttaaa ccgttgggaa
    47761 aatgagttaa caacgcctta ccacgcgttt ccacatgggt aacgtgttga ccgataagtt
    47821 gtgattgata agtttttaac tgcgggaagg caaaccagac atcagttagt ggtttgcctt
    47881 tgatcgccgc ctccaggtta tccgctgcac ggcggatctc cgggccttca ggcatggtga
    47941 tatccttttt tcattcttta ttaagcgggg ttatgctgcg acaacaatcc cctttttggc
    48001 aaatgtagct cgtaatcggc gggcgaacgc cagagcgtgc tcgccgtcgc catgcaggca
    48061 gaccgtttgc gccgcgaccg ttgcccattc gccggtgata cttttgactc tgccgtgttg
    48121 caccatctcc agcgtttgcg ccaacgcctg ttcttcgttt tcaatcaacg cgcctggctg
    48181 acttcgcggc accagcgagc cgtcagcctg ataaccgcga tcggcaaaca cttcctcgcg
    48241 cgttgtcagg ccatattgct tgcctgcacg aatcagctcg cttcctgcca gcccaacgag
    48301 aaccaatgcc ggatcgcaag cgtataccgc tctggcgatg gcgtctgcca gttgtgtttc
    48361 tttcgccgcc tggttgtaca acatgccgtg cggtttgaca tgacgcatta cgccgccttg
    48421 cgcacgggca atcgttgcca gcgcgccaat ttgatacagc gtctgggcgt aaacggtttc
    48481 cggcggcagc tgcatggcgc tgcgaccaaa attttccctg tcgggaaaac tcgggtgagc
    48541 gccaatcgcg acaccatttt ttattgcttc acgcacgcaa gcctgcatgg tttgcgcatc
    48601 gcctgcatga aatccacaag caatattggc agaggaaacc agcgttaata gctctgcgtc
    48661 gctggcgcag ccttcgccca gatcggcgtt caggtcaatt ttcattgtgc agccgccacg
    48721 ctaattgttc aaaataacgt tgctgatctt gccgcgcttt cagtgcctct tccagtgaac
    48781 actggacaaa atgaatcggc tgaccgagcg gaatttgcgc cagatggtac atatcagcct
    48841 caatgataca ggcaatacgt ggataaccac cggtggtctg tgcgtcgttc atcaacacaa
    48901 tcggctgccc gttatgcggc acctgcacca cgcccggtaa caaaccgtga gataacagtt
    48961 cgcgatcggt gatgcgtttt aaaatttgcc cctgtaaacg atagcccatt cggttacttt
    49021 gcgagctaag ctgccagggc gaacgccaga atgcatcctg cgaggcgcga tcgaactcat
    49081 gatattccgg ccccggcaag gcgcgaatgc ggttgcccca cagcagctgt ttaacgccct
    49141 gcgcttccat aaaatcacgc ttcgatttgc caatcgggag tcggtcacca tccttcagta
    49201 aacggccttc cagcccgcca atccccactt tgagatcggt gctgcatgac cccattaccg
    49261 gcggaacatc aataccaccc gcgaccgcca gataactgcg catcccgtgc tgcgggcgtt
    49321 taagcgttaa acgctggcct gctttcatcg gcaatcgcca gccggtccag acggcgttat
    49381 catccaaccg cgcttcgcaa cctgcacccg tcagggcaaa ccacccatca gtttcgaact
    49441 caactgttaa ctgaccgagc gtgatctcca gcgcgggggc attggcgtca ttacccacca
    49501 gcaggttagc aatgcgcagc gcaggcatat ccagtgcgcc acagtggctg atacccgact
    49561 ggcgaaaacc gtgacgaccg ccatcctgca cagtggtgta catgcccgca cgaataatct
    49621 tcagcatatt ccctccttct gcggtacaaa tcgcacgctg tctcccggac gtaataagat
    49681 gggttcgtca cgcgccggat caaacaggct gagtgaggta tgaccaatca actgccagcc
    49741 acccggcgtt gccagcggat aaacaccagt ctgcggcccg ccgatcccga cagaacctgc
    49801 cggaacgagc aagcgcggtt cagcgcgccg tggcgtgtgt agttgttccg gcaaactccc
    49861 gagatacggg aagcccggtt gaaaacctaa aaaccagacc acgtattcca cggaggagtg
    49921 caattcaaca acctgttttt cgctcaaccc gcaatacgcc gcgaccaccg ccaaatccgg
    49981 tccgcctgca ccaccgtaaa ccaccggaat ttcaataaag cgagactcag gttccagggc
    50041 ctcgctctcc tcccaccagc gttgcaaacg ctctatggca tccagcgcca gcgactcagg
    50101 attacgcaaa atcaccgtga tattgttcat gccgggaatg gcttcaacca cattcggcat
    50161 atccaccaga cgctgcgcca gtcgccagat ccgcttctgg ctagccagcg tcaccggcgg
    50221 ttccagttcc agcactaccg ccgtttcacc tatcagataa caacgcgctc gttgcacttt
    50281 cgatccctct gattattcgt tatgcaggat taggaatatc aataaaggtc acatcaagat
    50341 cggtattttc attcagccac tcgctcaatg cgcgaatacc accgcgttca gtggcatggt
    50401 gacctgcagc ataaaaatgc aatccctgct cgcgggctga atgaatggtc tgttcagaaa
    50461 cttcgccagt aataaaagca tccacgccaa aacgcgcggc gctatcgata aaactttgcc
    50521 cgccgcccgt gcaccaggcg acgcgctgga ccacctcagg tccggtatcg ccacaccata
    50581 acggcttacg tcccagacgc gcttcaatcc aggaggctag ttccattccc ggcacaggca
    50641 tggtcagctc tccccacggc accaacggct caatttcgcc catgaccgtg atccccagta
    50701 acgccgccag ctgtgcgtta ttgcccagct caggatgtgc gtcaagcggc aaatgccagc
    50761 catacaggtt gatatcattc tccagcaacg tttttaaacg gttacgcttc attccgcgaa
    50821 tgaccggaga ctctcctttc cagaagtagc catgatgcac aatgaccgca tccgcgccca
    50881 gacgcactgc ctcatcgagc aaagcctggc tggcggtgac accggtaaca attttttgca
    50941 ccgtctcttt gccttccacc tgcaaaccat tcggcgcgta atcactaatc gccgcgctgt
    51001 tcagtttttc gttaatcagt tgttccagtt cggtgttttt catcttcgcg ccttccgtat
    51061 tctgatagct acaccgttaa catagcgtta cctcgcctgt tcgtcgatag ccaatctttt
    51121 gacatattaa gaaacaaacc cgacgccaaa agacgcaaat tgtttatatc atcacgcttc
    51181 agctaatgta tatttatgca atcagacagc atattgcata aataatcaga tctgtatcaa
    51241 agttttaact ttaaggaaac acaagcacgc cgcgcaacta ttacgtgacg ttgccgtcaa
    51301 ttgtggaatt attatgaata aacacgcatc acaaccgcgc gctatttact atgtcgttgc
    51361 gctgcaaatc tgggaatatt ttagctttta cggcatgcgt gccctgctga ttctctatct
    51421 caccaatcaa ctaaaataca acgatactca cgcctacgag ttatttagcg cctactgttc
    51481 gctggtgtat gtcacgccaa tcctcggtgg ctttttggcg gataaagttc tcggcaatcg
    51541 catggcggtg atgctggggg cgttgttgat ggcgatcggt catgtggtgc tgggtgccag
    51601 tgagatccat ccgtcattcc tctatctgtc cctggcgatt atcgtctgcg gctatggcct
    51661 gtttaaatct aacgtaagct gtctgctcgg cgagctgtat gagccaaccg atccgcgtcg
    51721 tgatggcggt ttctcgctga tgtatgcggc gggtaacgtg gggtctatta tcgcacctat
    51781 cgcctgtggt tacgcccagg aagagtacag ctgggcgatg ggctttggcc tggcggcggt
    51841 tggcatgatc gcgggtctgg tcattttctt atgtggcaat cgtcatttca ctcatacccg
    51901 cggcgttaac aaaaaagtac tgcgtgcgac aaactttctc ctgccgaact ggggatggct
    51961 gctggttctg ctggtggcaa cgcctgcact gattaccgtg ctgttctgga aagagtggtc
    52021 ggtatacgcc ttaattgtcg cgactattat tggcctgggt gtactggcaa aaatttatcg
    52081 caaagcagaa aaccagaaac agcggaagga gctggggctg attgtgacgc tcaccttatt
    52141 cagtatgttg ttctgggcct tcgcacaaca gggcggtagc tcgattagcc tttatatcga
    52201 ccgcttcgtt aaccgcgata tgtttggtta taccgttccg accgcgatgt tccagtcgat
    52261 taatgccttc gcagttatgc tgtgcggtgt gttcctggcg tgggtggtta aagaaagcgt
    52321 cgcaggtaat cgtaccgtgc gcatctgggg aaaatttgct cttggccttg gcctgatgag
    52381 cgccggattc tgcattctga ccttaagcgc ccgctggtcc gcaatgtatg gtcactcttc
    52441 tctgccactg atggtgttag gcctcgcggt gatgggcttt gcggaactgt ttatcgaccc
    52501 ggttgccatg tcgcaaatta cgcgcattga aattccgggc gtgaccggcg tattaaccgg
    52561 catctacatg ctgctttctg gcgcgatcgc gaactatctg gcgggcgtga ttgccgatca
    52621 gacatcgcag gcttcgtttg atgcttccgg ggcgatcaac tactccatca atgcatatat
    52681 tgaagtattt gatcaaatta cctggggcgc actggcgtgt gtaggactgg tactgatgat
    52741 ttggctgtat caggcgctga aattcagaaa ccgcgcgctg gcgctggagt cataataggc
    52801 cagcaccgat gcctgatgcg acgcttgacg cgtcttatca ggcctacgta tttcctgcaa
    52861 tttattgaat ttgcacaaat ttgtaggctg gattaggcgt tcacgccgca tccggcatct
    52921 gggctcgatt gcctgacgcg tctgttattt ccccttccgc gccgcctcat acgctgccaa
    52981 cgtttgtact ctcgcctctt tgtgctcgac tatcggttgc ggataatcca gcttcacacc
    53041 tgctttctgc gcccacttcc acggctcatg caccgctttc cctggtacat cgcgcagctc
    53101 cggtaaccat cgacgaataa actcgccctc acggtcaaat ttctcgccct gagttatagg
    53161 attgaaaata cgaaaatacg gcgcagcatc agtacccgta gaagccgccc actgccagcc
    53221 accgttatta gctgccaaat caccatcaat cagctgcgac atgaaatatc gctcgccttc
    53281 gcgccagtcg atcaacaaat ctttcaccag aaaactggct gtaatcatcc gtagccggtt
    53341 atgcatccag ccagtgctgt taagctgacg catagcggca tcaataatcg ggtatcccgt
    53401 tttgcctttc tgccaggcct gtaaatgtgc gggattgctt tgccactgca cacgatccgt
    53461 ccaggcaata aatggacgat gtttacacaa cgaggggtaa tacgtcatca gatgacggta
    53521 gaactcgcgc cagatcagct cactaagcca gacactaccg gccccaccgt ccagcgcctg
    53581 cggctgttcc gccaacaagc gatgcaaaca ctggcgaggc gataacccgc ccgttgccaa
    53641 gctggcggac aaacggctgg tgccttccac tgccggaaaa tctcgttgtt gctcatactc
    53701 tccagcaccg ttctcgcaaa actgccgtaa ttgcgcaatc gccgcttttt cttccaccgg
    53761 gaaatgcgca gtatcgaaag actgacgagg ataattcagc gtaatggatg gcgcgggctt
    53821 tatcgatccg ctactacgaa cttttggtgc agcgacgcac tccggcatcc cttcccgcag
    53881 ccgtttcagc caggcattct taaaaggcgt aaagactttg tacatctcgt gattgccggt
    53941 catcaccgcc cccggcggca ggatcacgct gtcatcaaat ccttcacaca ccacgttacg
    54001 cagcgctctt tcaacgtcca catcccgcgc tcgctcattc acctcatact gatagttata
    54061 aaaaagatga gtaacgctgt tttccgcgca cacctgttta actatttcga cactggcgac
    54121 aaagtcatcc acttcacgga acaataaagg aatacctttt tccgccagcg cgatttgtag
    54181 cccattcagt tgagcattga taagttcagc ctgacgcggc gacatgttat gcgtctccca
    54241 ctggcgtggt gtagcgatat acagcgccag cacgcgtgca gacgaattgc ggcaggcggc
    54301 agccagtgcg agattatcgt gcagacgtaa atcctggcga aaccaggcca gatgggtagt
    54361 cataaaactc ctgaatggcg caagttaatc acacgcggcc aaagttcgaa ataacgctgc
    54421 cctgaaagcc aggcgtcagg ataaagcgcc atatagtgtt tgatacgcat cagcggcgca
    54481 agaagtggtt gctcgccacg gcgataactg tcgataagcg cagccagctg ctggcgttct
    54541 gtggaatcaa tatgcgggcg aaaataaccc tgaacgtgca tcaaaacgtt ggtgtgatcg
    54601 cgcgggttgg cgggatgaga aagcaaaaca attacgcgtt ggcggtactg gttgtaaaaa
    54661 tcatccagat tttgccactg atgaatatca gccacaaacg ggccaagttt gcggtactcg
    54721 ggctgagaat gggccagaaa gacgagttta tagcggctgt gataatcgag aagcgccccg
    54781 cgcgttaagc catgttcttt cagtcgatgt aactcatgaa gggcgaagat acggattagg
    54841 gtgctgtcat caaatcgttg tagattcatt actgctcctg aacggtatac aggagtaagt
    54901 gtagacgttg gtaaatgatg tttgtgaagt taatccgcta gcagggcaag attacgaaag
    54961 cgcgctcccc gcaagcaact aatcaaggta gttaatcatg gctgaacacg atattacgcc
    55021 agaagtcacc atttccaaaa cacagcgccg ctataaagtc ggctatgtca gagcccgcca
    55081 ccaggaccgc aaaaccggta tggcccggta ttatagcctg caccttaagg gcgactggct
    55141 gaaagaggcc ggatttgata ccggacgggg tgtcacggtg aagatttcgg aggggggcct
    55201 gaccatcatc gctgacagtg acgaggtgca ggaactgcgg gaggaacttt atcaggttaa
    55261 acaggtagtt aaggggatgc gggatgggat gtttagtgtg ctgaacgaaa gttaaatatt
    55321 aaaaaagctg ggaagattta aaaccttcct agctttttat tctgtcttat tataatgtat
    55381 actcatcatc ttccgatgaa tttataaatt gctccaagtt tacatgaaga gtacttaacc
    55441 tagtttttaa ctcattaagt gaagcgataa tttcatattt tccaacataa tcatatgatg
    55501 gatcgtattc attatctatg tcagaagcta aaaatgatga tattcgtagt ttaaatttag
    55561 cttcatcttt attacattca ttggcataag gtgaatgccc tatcatagca tattcctcta
    55621 atctaaaagg ataagatggt tttaattcat cgatcaatga aatttcaatc catgttctat
    55681 atccaggcat taatggacta atttctttga gcaaaacctt tccacttaat aatgtattga
//
LOCUS       DAETPZ010000018        55426 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.018, whole genome shotgun sequence.
ACCESSION   DAETPZ010000018 DAETPZ010000000
VERSION     DAETPZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.018"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..374
                     /locus_tag="K0W59_001208"
     CDS             <1..374
                     /locus_tag="K0W59_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3022329.1"
                     /translation="IAGYYMKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQIL
                     NAIFLGILCGIGMLYFQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHAL
                     FWLAIGMLGIAMICLLFIKDI"
     gene            485..1408
                     /locus_tag="K0W59_001209"
     CDS             485..1408
                     /locus_tag="K0W59_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBI3022330.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVKGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(1412..2230)
                     /gene="nlpA"
                     /locus_tag="K0W59_001210"
     CDS             complement(1412..2230)
                     /gene="nlpA"
                     /locus_tag="K0W59_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HBI3022331.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVQLVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            2452..2745
                     /locus_tag="K0W59_001211"
     CDS             2452..2745
                     /locus_tag="K0W59_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022332.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(2786..3976)
                     /gene="nepI"
                     /locus_tag="K0W59_001212"
     CDS             complement(2786..3976)
                     /gene="nepI"
                     /locus_tag="K0W59_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HBI3022333.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4187..4639)
                     /locus_tag="K0W59_001213"
     CDS             complement(4187..4639)
                     /locus_tag="K0W59_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HBI3022334.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHPPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(4692..6026)
                     /gene="adeQ"
                     /locus_tag="K0W59_001214"
                     /gene_synonym="yicO"
     CDS             complement(4692..6026)
                     /gene="adeQ"
                     /locus_tag="K0W59_001214"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HBI3022335.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVVLAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            6201..7967
                     /gene="adeD"
                     /locus_tag="K0W59_001215"
     CDS             6201..7967
                     /gene="adeD"
                     /locus_tag="K0W59_001215"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBI3022336.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(8013..9404)
                     /gene="uhpT"
                     /locus_tag="K0W59_001216"
     CDS             complement(8013..9404)
                     /gene="uhpT"
                     /locus_tag="K0W59_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HBI3022337.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEVSEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(9542..10861)
                     /gene="uhpC"
                     /locus_tag="K0W59_001217"
     CDS             complement(9542..10861)
                     /gene="uhpC"
                     /locus_tag="K0W59_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HBI3022338.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(10871..12373)
                     /gene="uhpB"
                     /locus_tag="K0W59_001218"
     CDS             complement(10871..12373)
                     /gene="uhpB"
                     /locus_tag="K0W59_001218"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HBI3022339.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(12373..12963)
                     /gene="uhpA"
                     /locus_tag="K0W59_001219"
     CDS             complement(12373..12963)
                     /gene="uhpA"
                     /locus_tag="K0W59_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HBI3022340.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(13038..13328)
                     /gene="ilvN"
                     /locus_tag="K0W59_001220"
     CDS             complement(13038..13328)
                     /gene="ilvN"
                     /locus_tag="K0W59_001220"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBI3022341.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(13332..15020)
                     /gene="ilvB"
                     /locus_tag="K0W59_001221"
     CDS             complement(13332..15020)
                     /gene="ilvB"
                     /locus_tag="K0W59_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HBI3022342.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPSFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQLRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPVAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPLGAANTEMVGE"
     gene            complement(15126..15224)
                     /gene="ivbL"
                     /locus_tag="K0W59_001222"
     CDS             complement(15126..15224)
                     /gene="ivbL"
                     /locus_tag="K0W59_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HBI3022343.1"
                     /translation="MTTSMFNVKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            15789..15878
                     /gene="tisB"
                     /locus_tag="K0W59_001223"
     CDS             15789..15878
                     /gene="tisB"
                     /locus_tag="K0W59_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HBI3022344.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            16158..17342
                     /gene="emrD"
                     /locus_tag="K0W59_001224"
     CDS             16158..17342
                     /gene="emrD"
                     /locus_tag="K0W59_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HBI3022345.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYKRTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(17350..17847)
                     /locus_tag="K0W59_001225"
     CDS             complement(17350..17847)
                     /locus_tag="K0W59_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBI3022346.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPSRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(17844..18206)
                     /locus_tag="K0W59_001226"
     CDS             complement(17844..18206)
                     /locus_tag="K0W59_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HBI3022347.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(18196..18543)
                     /locus_tag="K0W59_001227"
     CDS             complement(18196..18543)
                     /locus_tag="K0W59_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HBI3022348.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            18652..19065
                     /locus_tag="K0W59_001228"
     CDS             18652..19065
                     /locus_tag="K0W59_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022349.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(19112..20554)
                     /locus_tag="K0W59_001229"
     CDS             complement(19112..20554)
                     /locus_tag="K0W59_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBI3022350.1"
                     /translation="MVGCYSGKPLNTQNIDSLAAEGIRFNSAYTCSPVCTPARAGLFT
                     GIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWHLDGHDYFGTGECPPEWD
                     ADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETFTWAHRISNRAVDFLQQP
                     VRAEEPFLMVVSYDEPNHPFTCPVEYLEKYADFYYELGEKSQDDLANKPEHHRLWAQA
                     MLSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQRENTWVIYTSDHGEMMGAHK
                     LISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPTMMALADIEKPEILPGEN
                     ILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLVLNLFTSDELYDRRNDPN
                     EMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLRPWRKDARPRWMGAFRPR
                     PQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(20589..22304)
                     /locus_tag="K0W59_001230"
                     /pseudo
     CDS             complement(20589..22304)
                     /locus_tag="K0W59_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
     gene            22471..23364
                     /locus_tag="K0W59_001231"
     CDS             22471..23364
                     /locus_tag="K0W59_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBI3022351.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVLEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(23361..24175)
                     /locus_tag="K0W59_001232"
                     /pseudo
     CDS             complement(23361..24175)
                     /locus_tag="K0W59_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24175..25791)
                     /locus_tag="K0W59_001233"
     CDS             complement(24175..25791)
                     /locus_tag="K0W59_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBI3022352.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            26086..26760
                     /locus_tag="K0W59_001234"
     CDS             26086..26760
                     /locus_tag="K0W59_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBI3022353.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNGLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(26799..28460)
                     /locus_tag="K0W59_001235"
     CDS             complement(26799..28460)
                     /locus_tag="K0W59_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HBI3022354.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(28656..29084)
                     /gene="ibpB"
                     /locus_tag="K0W59_001236"
     CDS             complement(28656..29084)
                     /gene="ibpB"
                     /locus_tag="K0W59_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HBI3022355.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(29196..29609)
                     /gene="ibpA"
                     /locus_tag="K0W59_001237"
     CDS             complement(29196..29609)
                     /gene="ibpA"
                     /locus_tag="K0W59_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HBI3022356.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(29603..29675)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            29914..30246
                     /locus_tag="K0W59_001238"
     CDS             29914..30246
                     /locus_tag="K0W59_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBI3022357.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(30248..31385)
                     /locus_tag="K0W59_001239"
                     /pseudo
     CDS             complement(30248..31385)
                     /locus_tag="K0W59_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
     gene            31486..32504
                     /locus_tag="K0W59_001240"
                     /pseudo
     CDS             31486..32504
                     /locus_tag="K0W59_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(32465..33802)
                     /locus_tag="K0W59_001241"
     CDS             complement(32465..33802)
                     /locus_tag="K0W59_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3022358.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(33877..35025)
                     /gene="dgoD"
                     /locus_tag="K0W59_001242"
                     /pseudo
     CDS             complement(33877..35025)
                     /gene="dgoD"
                     /locus_tag="K0W59_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(35022..35639)
                     /gene="dgoA"
                     /locus_tag="K0W59_001243"
     CDS             complement(35022..35639)
                     /gene="dgoA"
                     /locus_tag="K0W59_001243"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HBI3022359.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(35623..36490)
                     /gene="dgoK"
                     /locus_tag="K0W59_001244"
                     /pseudo
     CDS             complement(35623..36490)
                     /gene="dgoK"
                     /locus_tag="K0W59_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
     gene            complement(36487..37176)
                     /gene="dgoR"
                     /locus_tag="K0W59_001245"
     CDS             complement(36487..37176)
                     /gene="dgoR"
                     /locus_tag="K0W59_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HBI3022360.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            37454..38110
                     /locus_tag="K0W59_001246"
     CDS             37454..38110
                     /locus_tag="K0W59_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022361.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(38156..38968)
                     /gene="yidA"
                     /locus_tag="K0W59_001247"
     CDS             complement(38156..38968)
                     /gene="yidA"
                     /locus_tag="K0W59_001247"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBI3022362.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMGNAIPSVKEMANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(39082..39480)
                     /locus_tag="K0W59_001248"
     CDS             complement(39082..39480)
                     /locus_tag="K0W59_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HBI3022363.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(39720..42134)
                     /gene="gyrB"
                     /locus_tag="K0W59_001249"
     CDS             complement(39720..42134)
                     /gene="gyrB"
                     /locus_tag="K0W59_001249"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBI3022364.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(42163..43236)
                     /gene="recF"
                     /locus_tag="K0W59_001250"
     CDS             complement(42163..43236)
                     /gene="recF"
                     /locus_tag="K0W59_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBI3022365.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(43236..44336)
                     /gene="dnaN"
                     /locus_tag="K0W59_001251"
     CDS             complement(43236..44336)
                     /gene="dnaN"
                     /locus_tag="K0W59_001251"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBI3022366.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(44341..45744)
                     /gene="dnaA"
                     /locus_tag="K0W59_001252"
     CDS             complement(44341..45744)
                     /gene="dnaA"
                     /locus_tag="K0W59_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBI3022367.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLSLYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            46041..46121
                     /locus_tag="K0W59_001253"
     CDS             46041..46121
                     /locus_tag="K0W59_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022368.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            46351..46491
                     /gene="rpmH"
                     /locus_tag="K0W59_001254"
     CDS             46351..46491
                     /gene="rpmH"
                     /locus_tag="K0W59_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBI3022369.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            46508..46867
                     /gene="rnpA"
                     /locus_tag="K0W59_001255"
     CDS             46508..46867
                     /gene="rnpA"
                     /locus_tag="K0W59_001255"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBI3022370.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            46831..47088
                     /gene="yidD"
                     /locus_tag="K0W59_001256"
     CDS             46831..47088
                     /gene="yidD"
                     /locus_tag="K0W59_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBI3022371.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            47091..48737
                     /gene="yidC"
                     /locus_tag="K0W59_001257"
     CDS             47091..48737
                     /gene="yidC"
                     /locus_tag="K0W59_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBI3022372.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            48843..50207
                     /gene="mnmE"
                     /locus_tag="K0W59_001258"
     CDS             48843..50207
                     /gene="mnmE"
                     /locus_tag="K0W59_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBI3022373.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            50449..50523
                     /gene="tnaC"
                     /locus_tag="K0W59_001259"
     CDS             50449..50523
                     /gene="tnaC"
                     /locus_tag="K0W59_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HBI3022374.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            50744..52159
                     /gene="tnaA"
                     /locus_tag="K0W59_001260"
     CDS             50744..52159
                     /gene="tnaA"
                     /locus_tag="K0W59_001260"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HBI3022375.1"
                     /translation="MENFKHFPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            52250..53497
                     /gene="tnaB"
                     /locus_tag="K0W59_001261"
     CDS             52250..53497
                     /gene="tnaB"
                     /locus_tag="K0W59_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HBI3022376.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWSAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGARLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            53629..54804
                     /gene="mdtL"
                     /locus_tag="K0W59_001262"
     CDS             53629..54804
                     /gene="mdtL"
                     /locus_tag="K0W59_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HBI3022377.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            54779..>55426
                     /gene="yidZ"
                     /locus_tag="K0W59_001263"
     CDS             54779..>55426
                     /gene="yidZ"
                     /locus_tag="K0W59_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HBI3022378.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTW"
BASE COUNT        13608 a        14753 c        13938 g        13127 t
ORIGIN      
        1 taatcgcagg ctattacatg aaacgtattg gcaagcgact attaatgctc attgctatcg
       61 tgagtggtat gtgtttttac gccagcgtac tcatggcgac gactccggcg gttgagctgg
      121 aattgcaaat tctaaatgcc atcttccttg gtattctctg tggtatcggc atgctttatt
      181 ttcaggactt gatgcctgaa aaaataggct ctgcgacaac gttatatgca aatacttcac
      241 gcgtcggctg gattatcgcc ggctctgttg acggaattat ggttgaaatc tggagctacc
      301 atgcgttgtt ctggctggcg atagggatgt tgggtattgc gatgatttgc ctgctgttta
      361 ttaaagatat ttagctcacc agtctgggct aagaaaaatc ggcgtggcgc aaacagagcc
      421 atcctcgcca tttttgctat catgcctgca tacataaacg acaaaacagt atgcagaggg
      481 aaaaatgggt tccaccagaa aggggatgct gaacgttctg attgccgccg tgttgtgggg
      541 aagttcaggg gtctgtgcgc aatacatcat ggagcaaagc cagatgtcgt cgcagttttt
      601 gactatgacg cgtttgatat tcgccggttt gattctgctg acgctgtcat ttgttcatgg
      661 cgataaaatc ttttctatta ttaacaatca caaagatgcc attagtctgc tgattttttc
      721 cgtggttggc gcgctaaccg tacagctcac ttttttgcta accatcgaaa aatcgaacgc
      781 agccacggca acggtgctgc aattcctctc accgacgatt atcgtcgcct ggttctcact
      841 ggtacgtaaa tcgcgcccgg gcattctggt tttctgcgct attttgacat cgctggtcgg
      901 gactttttta ttggtgacac acggtaatcc gacgtcatta tcgatctctc ctgccgcgtt
      961 attctggggc attgcctcgg catttgctgc tgcattctat accacctatc cctcaacgct
     1021 gattgcccgc tatggcacgt tgcctgtcgt cggctggagt atgctgatcg gcggtctgat
     1081 tctattgcct ttttatgcca gacaagggac aaactttgtc gttaaaggca gtttgatttt
     1141 ggcgtttttt tatttgatcg tcattggtac gtccctgaca tttagtctgt acctgaaagg
     1201 agcacaatta attggcggtc caaaagccag cattttgagc tgtgcagaac cattaagtag
     1261 cgcgctactc tctttgctgt tgctggggat cacgttcaca ttaccggact ggctgggaac
     1321 gctgctgatt ctgtcatcgg taattttgat ttcaatggat tcccgtcgcc gcgccagaaa
     1381 aataaatcgt ccggcgcggg atgagtgaga attaccagcc aggcaccgcg ccaccgttaa
     1441 agatggtttc tgccgcttta gcgacttcgg atgattgata agattgcaga aattccttca
     1501 cgttttctgc attcttatta tcttcccgtg ccaccaaaat attcacatac ggcgaatttt
     1561 tatcttcaat aaatacactg tcgtgcaccg gagaaagccc ggtctgctga atgtaagtgg
     1621 tgctgataat cgccacatca actttgggat catccagtac gcgcgggagc tgcgccccct
     1681 cgagttccat aatctgcaaa tgacgaggat tatcggttat atccagcgcg gtaggtaata
     1741 acccttttcc ctctttcaga gtaattagct tctctttttg caacagtaaa agcgcacgac
     1801 caaggttggt cgggtcgttg gggatcgcca ctgtcgcgcc ttcttttatt tgcgccacgg
     1861 ttttgatttt tttggaataa cccgccatgg ggaagacaaa cgtatttccc accgctacca
     1921 gtttataacc atgcgcctga ttatcttgtt caagaaaggg tcgatgctgg aagacgttcg
     1981 catcaagttc gccatgattt gtcgcatcgt tcggtagcaa cgaaccgcta aatcctacca
     2041 gttgaacatc gaggccatat ttctcttttg ccaccttttt agcgacttcc gcgacatctt
     2101 gttctgcgcc atttataacg ccaactttaa tgtgttttgc atcgctgcta ctctggtcgc
     2161 aacctgccag cagaattccg gccagcaata atgcggcccc tgtccgtaga tgatgtgttg
     2221 tcagtttcat ttttttatcc ttttattaat gattacctga ctatagcggt aaggtcgctg
     2281 cggtttaaaa aacgaaacgc tatcgataag aataaaaagg aataaaagtg gaatataaag
     2341 tctggcactt cttactgacc acgcaagcgc gtttcgtaca gcatgatgag agcgatgaaa
     2401 gcaaactaca cttatgcttc attcgataca catttgtaaa aggttgaatc catgaaacca
     2461 acgacactac tgcttatttt cacttttttc gctatgccag gcatcgtata cgccgagtcg
     2521 ccttttagct cactgcagtc cgcaaaagag aaaaccacgg tattacaaga tttacgtaaa
     2581 atttgcacgc cacaggcgtc attatcagat gaagcgtggg aaaagttaat gttgtcagat
     2641 gaaaacaata aacaacatat acgcgaggcc atcgtggcga tggaacgtaa taatcagagc
     2701 aattattggg aagcgttggg taaggtagaa tgcccggata tgtaagaaca gcaacaacta
     2761 ctgaccaacg cttctttacc tgatatcagg atttcttcat tttcaccttt gcagtaacca
     2821 acaatgcagt cagcaacatc aatgtgccgg acaacatcaa cggcgaagtc agaccaatat
     2881 tatccagcgc ataaccgccg attgccgcgc cacaggtatt agcaagctga ataaccgcca
     2941 cctgaataga cccggctttt tctgcctgat cggccagcga gcgggtgatc cacgttgacc
     3001 agccgacggg aaccaatgca aaagttagcc cccagataat cgccacgccg gtagcaacga
     3061 ttttatcgct tccccacaac gtcagtacca acgcactcac agccagtatt aacggcgcgc
     3121 ctgctaaggc cagttttacc gaacgtttaa gaatgaacga cgaaagcgac gtaccaataa
     3181 agctggcgat accaaaactc aacagcacca gcgttaagcc atccacgccg aatcccgcca
     3241 ggttcatata caccgggcga atatacgtga agaaagcaaa ctgcccggcg aaagacatga
     3301 agatggcgat catccctgcc atcacacccg gacgttgtaa taagcggaaa gtattttgtt
     3361 tctgatgcga gggttcgcct ggcagtgaag gcaatgattt gataatccag aaaatacaca
     3421 gcacgcccat caccgccgcc gcattaaaga cattgcgcca accgataagc tcgcctaaaa
     3481 aactgcccaa cggcgcggca atcaccagcg caatagaaac cgcgccgaag atcaccgaca
     3541 gcgccttcgg caccgtacgc ggcggtacca gacgcatggt cagcgacgcc gacatcgccc
     3601 agaacccgcc cagcgccagc cccagacagg cacgaccgat taaaagcaaa ctgaatgagt
     3661 tagcaaagga aaccagcaag caggagagcg tcagcaaaac ggcaaacaaa ataacaacgt
     3721 agcggcggtc agtggcctga attgtctggg taataaacaa actggcaaac attgccacaa
     3781 aggcggtcac ggtcaccgat tgcccggcaa ccccttccga aatgcctaaa tcctgggcca
     3841 ttggcgtcaa caaactgacg ggcaaaaact caacgataat cagacaggcg acacaaaacg
     3901 ccaccgagaa aacggctgac cagttcggtc gggtgatggc atccgcgccg cggttttcgg
     3961 caataaattc actcatggtg ttacccgtgg catgtttcag gggaaaagcc gaccacttta
     4021 acattgaaat gtgtgacgca tttaacgttt ttgcaacttt tacggtgttg cttgagctgg
     4081 agttaagatc gaaacggaac agggcaaaga ttaccggatg cggcgtgaac gtcttatcca
     4141 cccgacaggt caggcgcggt ctgtaggctg ataagacgaa acggtttcaa tgcaaccagt
     4201 gaacgccctc ggcaggctga aagaacgcgt tatagcgcat ctgaaaagca ttaatatcct
     4261 gcatttcagg ttcggtttca cgcagaaaac ccagcgccag cctgacctga gcatcggtaa
     4321 tcggcgcgga aaggcgggct ttttgtaaaa tccggtgcca ttgcagcagg ttctgttcgc
     4381 tatcatcgac aatgacaatt tgccagataa gcaacacctg cgcggcgttt tgcaggtgcg
     4441 actcatccgg gggatgcaaa taacgtaaaa attcgtcacc ggctgacttt cccatttgat
     4501 cgaccatcga ctccacgggt acgacatcga tattcagccg atcgctgaga cggcgaatcg
     4561 cttttctggc cccggatgtc agcacccgaa aaccaaccac aaacactacc gccagcgtgg
     4621 cgagcattat ccagatcatg agttctcctg cgtaatcaga actcatgata tctggaaacc
     4681 ctcgccagtt actaatccac cagaataatc ttcaatgcga acagagctgc gaccaccacc
     4741 acacacaggt tcagatcgcg ccagcgcccg gtgcacactt tcatgatgca gtacgacata
     4801 aagccgagtg caatcccttc ggtgatcgag aaagtaaagg gcatcatcac cgtggtaata
     4861 aacgcaggca ccgattcggt aaaatcatcc cagttaacgc gcgccaggct cgaagtcatc
     4921 agcacgccaa caaagattaa cgctccagcg gttgcgtaag gaggaaccat cgccaccagc
     4981 ggtgagaaga acataactaa caggaacata acgccgacca cgacagccgt tagccccgtg
     5041 cgaccaccga ctgccacacc agaagtactt tcaatatagg cagtaacaga cgaggtaccg
     5101 ataaatgcgc ctgccaccga actgacgcta tcaacataca gcgccttatt catattgggg
     5161 aatttaccgt tgctatctat taagcccgct ttatcagtta caccaattaa tgttcctgat
     5221 gaatcaaata ggttgatcag cataaaggag aaaatgatac cggcgagttc aagtgttaac
     5281 gcgccgctca aatctacttc accaatgacg ccgctaatat caggcggaat ggaatagacg
     5341 ccgctaaaat gaacatcacc gaaaaataat ccacagcaag acgtcaccac aatagaaacc
     5401 agcaccgcgg catgaaaatg acgtgatgac aacacggtta taataaaaaa ccctaaaata
     5461 cctaacaaca cgccgtgaga acttaaatcg ccaatcatca ccagcgtgtc tttattggcg
     5521 acaataacac cagtgttttt taatcccatt aaggcaataa ataatccgat tccactggtg
     5581 ataccaatac gtaaacttaa tggaatgttg gagatcatcc agtaccggat acgaaacagc
     5641 gtgagcaaaa atagcccaat tgcgccccag aatatcgcgc ccatcccggt ctgccaggag
     5701 atccccatcg cccccacgac cacgaacgca aagaaggcgt tcagccccat tgccggagcc
     5761 agcaccacgg gtaagttagc aaatatcccc atcgcaatac tgccgatacc ggcaatcaaa
     5821 caggtggtaa caaacaccac tttcgggtcc atttgtgccg cgccgaggat ttgcgggttc
     5881 acaaaaacaa tgtacaccat ggttaaaaaa gtggtcatcc ccgcaatcaa ttctgtgcgg
     5941 acggtggtcc catgctgagg tagtttaaat aatcgcgaaa gcgtccctga ttcattactc
     6001 acgtaatcgg tattgtcatt attcattttt ttgtccattg gaggagattt aatcccttgc
     6061 ttttgtaaaa caaatgacaa caacggttca gtgataatta tcacatttca attgcacatt
     6121 aatagatatt ctttaataat ctcgcgacgt ttctttatga taaataataa tcaaattgat
     6181 aaaatcaaaa tgagaaaaat atgaataatt ccattaacca taaatttcat cacattagcc
     6241 gggctgaata ccaggaattg ttagccgttt cccgtggcga cgctgttgcc gattatatta
     6301 ttgataatgt ctctattctc gacctgatca atgccggaga aatttccggc ccaattgtga
     6361 ttaaaggacg ttacattgcc ggtgttggcg cagaatacgc tgatactccg gctttgcagc
     6421 ggattgatgc tcgcggcgca acggcggtgc cagggtttat tgatgctcac ctgcatattg
     6481 aatccagcat gatgacgccg gtcacttttg aaaccgctac cctgccgcgc ggcctgacga
     6541 ccgttatttg cgaccctcat gaaatcgtca acgtgatggg agaagccgga ttcgcctggt
     6601 ttgcccgctg tgccgaacag gcaaggcaaa accagtactt acaggtcagc tcttgcgtgc
     6661 ccgctctgga aggctgcgat gttaacggtg ccagttttac ccttgaacag atgctcgcct
     6721 ggcgggacca tccgcaggtt accggccttg cagaaatgat ggactaccct ggcgtaatta
     6781 acgggcagaa tgcgctgctc gataaactcg atgcatttcg ctacctgacg ctggacggtc
     6841 actgtccggg tttgggtggc aaagaactta acgcctatat tgctgcgggt attgaaaact
     6901 gccacgaaag ttatcagctg gaagaaggac gccggaaatt acaactcggc atgtcgttga
     6961 tgatccgcga agggtccgct gcccgcaatc tcaacgcact ggcaccgttg atcaacgaat
     7021 ttaacagccc gcaatgcatg ctctgtaccg atgaccgtaa cccgtgggag atcgcccatg
     7081 aagggcatat cgatgcctta attcgccgcc tgatcgaaca acacaatgtg ccgctgcatg
     7141 tggcatatcg cgtcgccagc tggtcgacgg cgcgccactt tggtctgaat cacctcggct
     7201 tactggcacc cggcaagcag gccgatatcg tcctgttgag cgatgcgcgt aaggtcacgg
     7261 tgcagcaggt actggtgaaa ggcgaaccga ttgatgcgca aaccttacag gcggaagagt
     7321 cggcgagact ggcgctgtct gccccgccat atggcaatac cattgcccgc cagccagttt
     7381 ccgccagcga ctttgccctg caattcacgc ccggaaaacg ctatcgggtc attgacgtca
     7441 tccataacga attgattacg cactcccgct ccagcgtcta cagcgaaaat ggttttgatc
     7501 gcgatgatgt gtgctttatt gccgtacttg agcgttacgg gcaacggctg gctccggcct
     7561 gtggtttgct cggcggcttt ggcctgaatg aaggcgcgct ggctgcgaca gtcagccatg
     7621 acagccataa tattgtggtg atcggtcgca gtgccgaaga gatggcgctg gcggtcaatc
     7681 aggtgattca ggatggcggc gggctgtgcg tggtacgtaa cggccaagta caaagtcatc
     7741 tgccgttacc cattgccggg ctgatgagca ccgacacggc gcagtcgctg gcggaacaaa
     7801 ttgacgcctt gaaagccgcc gcccgtgaat gcggtccgtt acccgatgag ccgtttattc
     7861 agatggcgtt tctttctctg ccagtgatcc ccgcgctaaa actaaccagt caggggctat
     7921 ttgatggcga gaagtttgcc ttcactacgc tggaagtcac ggaataataa aaaaagcccg
     7981 gcgtcatgcc gggcaaaagt caccagttac gtttatgcca ctgtcaactg ctgaattttt
     8041 ttctcgcggc ggattttgcg ttcttccatt accgctacta tcgccatcag gcagatacaa
     8101 ccaatcgcgg cgatatccag cgcggcgaag gtgcctgccc agccggtaag gccgaatacc
     8161 ggcgtcccat cggcaatcat tcccagacct aacttggcaa agctgtcacc aatcaggtaa
     8221 gcaaaggtgc ctttaatacc atcggcagcg ccaatcgctt ttttaggtac aaagccaaca
     8281 gcagccacac caatcaacaa ttgcgggcca aagaccagga aacccaacgc aaagagagaa
     8341 gccagataga tatattcgtt actggcatgt tgatacacac cgagcgtggc gataatcagc
     8401 gccagcgcga tgcaggccac caggccacgg cgaccgttcg ccaggtcaga gagccagccc
     8461 cacagcagcg taccgaccag cgcaccagct tcaaacagcg taaagccctg aatcgccacc
     8521 gctttagaga gtttcagttc ctggaacgcg tatacggttg accactggtc gataccaata
     8581 cgtaccacat agaggaaaat gttggcgaag cacagcagcc agatcacttt gtttttcagc
     8641 acatactcaa caaagatctg ccacttggtc atatcggtag attctgtctc tttgtcctct
     8701 tcgctgacct cctcgccgaa cagttcttca gctttgccga ggccataaga ttccggggag
     8761 tcgctaccgt aacgcaggcc gataaaaccg acaatcagcg cgataatcga cgggaagata
     8821 aacatgccga tgacatggcc atcgaacagg taatttgccc cgaacagcgc cacacctgct
     8881 gcgcctgcac cgccaaggtt gtgagaaata ttccagaaac cgaggaatgt cccgcgttta
     8941 cgacgcggcg tccatttggt gatggtggag taactgcacg aaccgccggt actctggaaa
     9001 aagccgctta aggcgtagaa ggcaatcatc aggaacaggc taaccgagcc gctgcccata
     9061 ctggcactga agcccagcat acaaatggca gagaggataa gcatgaacgg caggaattgt
     9121 ttggtgtttt tgccgtcggc gtagtatgaa accaacgttt tacccacgcc ataagtgatg
     9181 gagaaaccca ggccgatcat ccccagctgc gtcatgctca acccgtaggt cgaaatcata
     9241 tcgttctgcg cgatgttaaa gttcttgcga atcaggtaca tcgtcaggta gccgataaag
     9301 accaccaggt aggattgcat gaacggtttg aaccacattt tgcgccgcac ttcgagcgga
     9361 aggtccaggg tcggcttgcg aacctggttt aggaaagcca gcatgggtta ctcctgaaat
     9421 gaatacctgc gtgaggcatg cattgtaaaa atcagcagca aatcaggcct gagatagcgt
     9481 ccaagaaaaa cctggaaaat ttcttagttt tgccggatat gaagtgaaaa ggtgagatgc
     9541 atcacgcttc acgcggtgtc tgggcgttca aaaagggcag taacagcagt gcggaaatcc
     9601 cggcggcgat agagataacc acaaaaaatc cgctccagtg ccaggtatcg agcactttcg
     9661 ccagcggcca accagcaagc gacgccccca gataagcaaa caagccgaca aaccccgtcg
     9721 ccgcccctgc cgcctctttg tgggaacact ctgccgccgc cataccaatt aacatctgcg
     9781 ggccaaagac aaaaaaacca atggtgaaga agcaggttgc ctgcatcacg tagctggcaa
     9841 atggcatcag ccacagcgag ccgactgaaa gcaaaattcc ggcggcgaaa atcaaattca
     9901 tcggcccacg gttgccgtta aacaatttgt ccgagcccca accggctacc agcgcaccga
     9961 taaatccgcc cagttcaaac atcgtcactg ccgtattcgc cgtgaccaga tcgacgccca
    10021 gcgtctcgga catatacaaa ttgccccagt cgttgatcgc cgcccggacc acatagacca
    10081 gcacatagca aaacgaaagc agccagatat acggattcag caacacatat ttggtgagga
    10141 tctctttacg cgtcaaccct gccccttctt gttgttgagc aatttccagc gcgtcgtgcc
    10201 gccattcacc gaccgccggt aaacctaacg cctgcgggcg atcgcgtagc cgccagcaga
    10261 gaaaaatccc cacgactatc gccatacaac cagcaatcat catcccggca cgccagccgt
    10321 aatgcagcgc gctcgctgcc atcacaatgg gaatgagtgc gccgccgacg ttatgcgccg
    10381 tgttccataa tgcccaccaa ccgccgcgct cggtacgtga ataccaggcc gttaacagac
    10441 gcgcacacac cggtgaaccc cagccctgga aaaaggcgtt cagcacccag agcacggcaa
    10501 acgcccatag cgacgtcgag aagccaaaca gaatgttcat gatgcccgtg gcgataagcc
    10561 ctatccccat aaaataacgg gcatttgagc gatcgctgac aatgccggag acaaacttcg
    10621 acacgccgta agtaatgtaa aacagggtcg ctaacaggcc gatatcgcta cggctgagca
    10681 cgccgttagc aaggatttct ggtaccgcgg cgttaaaact tttccgcgtg aagtaaaaca
    10741 gcgcgtaacc cagccagatg gtcagcagaa tatgccgacg ccagtagcga taacgggcat
    10801 caatttcatg tttatcagtc attaatggcg catcggcagg cgctttcaga aacggcaaca
    10861 tcatcgaacc ttagacataa cgttgaggta gagaaacgct gacacgcgtg ccgtgcagac
    10921 aggaaatgtg taatgtgccg cccagcgccg ttacgcgctc gcgcattccg gtgaggccaa
    10981 aaccttgttg cccggaaccc ggcggcaaac cgctgccatc gtcttcaatc accagcatca
    11041 accgttcatc ctgctgccag ccttgcagcg taaccgcgct ggcatcggca tgtttcacaa
    11101 tgttgttcag cccttcctgg cagacacgaa acagcgtcac gcgctggttt tcgcttaatg
    11161 ctgattcatc gatacgccat tcgagatggc tgacaatgcc gcgcccttcc agctccattt
    11221 cccgcatcag tgagcggatg gcctgctcca gggtgagatc atccagctgg cgcggacgta
    11281 accgtcctaa caaacggcgc accgcgtcgt aaacgcccag cgacagttgt tcgatgagct
    11341 gcccgctctg cttcacactg gcgttatctg ccgccagccg ctgaacaatg cccgcctgag
    11401 tacgaatagc agtgatggtc tgaccgatat cgtcgtgcag ctcacgcgcc acgtcgcggc
    11461 gcacgctctc ttcggtttct aacaaacgtt cagccagatg ctgattgcgc gccagttcct
    11521 tttgtagcga ctggttaagt tcacgcaacc gctggatgcc tgcgcccagt aacaaccctg
    11581 tcagactttg caccagcaac gagagtaata aatccaccag atgatcgcgc caggtttgac
    11641 tggcgatcag cgcgatggcg ttcatcaacg tcgcaatcag cgctccttgc cagccatagt
    11701 gccaggccag cgcgataatc ggcagcgcca gacagaatgg cgtaaagcgc gacagttcgt
    11761 ccggcaatcc caactggagc cagagactga taacaaacag cagcaagtac cagaccagat
    11821 gccgcccgcg ccagttgatg ggctgagaaa ccagtgacgg gccgagcggc agccaggtgt
    11881 tattggcgag atagtgccag aacaccagac atatcggggc cagcgtcaga ccgccagtta
    11941 aagtcagcaa cagcgcattc cacgactctt tgccgtgcca aagccagggc agcgactgca
    12001 acaacgccgc cgccgttaac gccgcaccct gtaacagcaa ggtgcgccag tcacgctgat
    12061 ggcgatagcg cgagatcagc gctacgggca gtaacgtcag taaactaccg atcatcaata
    12121 acggaaaatg ggttaaaccg accgcctgcg ttagccagta aatcagcagc cactccgcac
    12181 ccagcaatac gggccagtat ccgcgcgggc attgcagcat tagccccaga cgcagaccaa
    12241 acggaaataa cagcaccgcc atatcagggc gctcaaccag atgcaggctg atactccata
    12301 ggcaaaacca tgcggcagag aagataaaaa agcaggcaat aacggtaatt aagcgggaga
    12361 acaacgtctt catcaccagc catcaaacat gcggcgcgcc agctctacgt cgttactgac
    12421 gcccagtttt tccatcagat tggcgcgatg gacgtgtacc gttttcggtg acaagcccag
    12481 ttcggcggca atctctttca ccgccattcc ttgcgccagt ttttccgcca cctggcgttc
    12541 acgtttggtc agcgggtcct gacgaccgga tgccagttta atggcaatat ccggcgtcag
    12601 ataacagccg cccgtggcaa ccgtatgcac cgcagcaatg agttcatccg ggctacagcg
    12661 tttggaaaga aagccgcgtg cccccgcgtt aagcgcctgc tcaaccagcg caggactgtc
    12721 gtgaacggag agcataatcg tcgccatacc tttcggcagc tggcttagca gctccagacc
    12781 ggagatatcg ggcatggaga tatcgcaaat acacacctgc acaccgcgcc ccggcagccc
    12841 cgccagcgcc tcgcgccccg aaccaaactc ggcaactacc tgcaaatcag gttccagccc
    12901 cagcagctgc gcaaagccgg agcggacgat gaggtgatcg tctataaggg caacggtgat
    12961 catggtcttg tcctggcggg taaaaaaaaa cgcgcttacc ttaacgataa gcgcgatgtt
    13021 gttcaagcct tgagcggtta ctgaaaaaac accgcgatct tgttaaacat cgtcggatcg
    13081 gactgattac gctgcacttt cacgacatct tccagcttat cgatttggct tatcatctgc
    13141 tccagacgct ggtcgtcatt gaccagtagc cagatatggc ttttgtcgct gtcctgaatc
    13201 ggcagacaaa gaatgccttc aacgttaaaa gcgcggcggg caaaaaggcc acaaacgtgg
    13261 gtcattacgc ccggatggtt gcgaacggtg agctccagaa ttacgttgtt atgagttgtg
    13321 ttttgcatgg cttattcccc caccatttca gtattcgccg cacctagcgg caccatcggg
    13381 taaacttttt cttcggcatc aatgcgcaca tggatcagcg ccgggccagg gcgattgatt
    13441 atttcctgca atgcagcctg cggatcggct tcgttattca aatcacaggt ttcgaggccg
    13501 aatccggcgg caatctgcat aaagttgatt ttgcccggat aggtggcggc aaaaacgcct
    13561 tgctcgtaga acagactctg ttgctgatgc accagcccca gcgcttcgtt gttcatcaga
    13621 atgattttga catccagctg attttcactg gcggtcgcca tctcctgaat attcatcatc
    13681 aggctgccgt cgccggagaa acacaacact ttgcgatccg ggttcgccag cgccgcgcca
    13741 atcgccacag gcaggccaaa acccatcgtg cccagcccac cggaggtcag ccactggcgt
    13801 gggcgattga gcggataagc ttgcgcggtc cacatctgat gctgacccac atcggtggtg
    13861 ataatcgcat tgtcatcgac acaggcggca acggcgttga tcaggccgta atggcttaat
    13921 ggatcgcacg ctttcgggat tggacacggg aattcgcgct gcaaatccgc taccaactgg
    13981 tgccactctg cacgcagttg cgcttccacc tgcgggatca attgcgccag cacgtcatca
    14041 acatccgcct gaatcgccac atgcggctgc ttgattttac ccagctctgc gcgatcgata
    14101 tcgacatgaa taattttggc attcggacag aactgctcgg ttttgccaat cgcccggtca
    14161 tcaaaacgcg caccgagcac aatcaacaaa tccgcctcct gcaaaatata gttggtactg
    14221 cgcacgccgt gcatccccag catacccagc gacaacggat gcgcttttgg cagcatgccc
    14281 agcgccatta aagtcatggt ggtaggcagt tgcgctttct ccgccagttc acgcacccgc
    14341 gcaggcgcat tgatcacacc accacccaga taaagcaccg ggcgtttggc agcgttaatc
    14401 attgtagctg cgtcacgaat gctttcttcg ctaaaggagg gtgcagcggc tttttctgcc
    14461 accgcgggct gagcttcaat ctcaaaaacc gccgtttgta catccttagg aatgtctatc
    14521 cacaccgggc tagggcggcc tgattgcgca atgcggaagg catcgctcat gacctgtggg
    14581 agctcttcga tatgtctgac cagatagttg tgtttggtga tggggataga gatgccgtag
    14641 gtgtccactt cctggaaggc gtcggtgccg atcatcgagg cgggaacctg accagtgatg
    14701 caaatcagcg ggatggagtc cagccgcgca tcagcaatgg cggtcaccag gttagtcgca
    14761 cccggcccgc tacaggccat acagaccgcc ggtttaccgt cggtgcgcgc cattccctga
    14821 gcgataaacc ccgcgccctg ttcatggcga gccagaatat ggcggatttg cgtgctttgg
    14881 cttaaggcgt cgtaaacagg caggatagaa ccgcccggaa tgcccgtcac aatcttaatg
    14941 ccctgctgtt ccaggaaatg aacgataaat tctgcgccgg taaagcgctt acgcgtcgat
    15001 gttgtgcccg aacttgccat gctccagtcc ttttcttctg ggccgacttt ccgggaggtg
    15061 cttaaacgaa aaaccccgcc cggtttgcgc cggcggggtt ttggaatcgt gtgttgttcc
    15121 agtccctacg gcgcattgcc gacgaccacc accacacgca cgacgaccac tgcggcggat
    15181 ggcgcagttg gtagtagttt tacgttgaac atggaagtag tcattgggga ccttgttggt
    15241 tttgtgttta acaatattta tacaagcaca gctttacagg ggagacaatg gaaaattttt
    15301 cagcaaggga aaattgaggg gttgatcacg ttttgtactg aattgcagat aacaaaaaac
    15361 cccgccggag cgaggtttcg tcagccgcct gcggctggta accgcaaagc acactgtatt
    15421 atgtcaacac tgaaagtata cgtgttccgc gcagaacgcg caatttcggc acgaattttg
    15481 acgtatttag tgcatagttg agtatcgttc acagtttgcg ttttgtccaa atattactgt
    15541 ttatttatac agtaaacttc tataatatca ctgttcgcaa tgtgttatgc gggggccgca
    15601 tcgttacccg gcgcactaag tcctggctga aacgggtggt gccgtcagcg ccttaacccc
    15661 gcgtgagcac actgtgttat gtcaacaagc acaacgtttc tccttgagat accgcgtgca
    15721 caacagctgg caacaggcag cggaaaggta cgtcagctgg cagtgctcct gaaccacagg
    15781 agacgcgtat gagcctggtg gatatcgcca ttcttatcct caaactcatt gttgcagcac
    15841 tgcaactgct tgatgctgtt ctgaaatacc tgaagtaatt cagattcaag tcgcaccaaa
    15901 ggggagcggg taaccgctcc ccttttatat ttagcgtgcg ggttggagtc ggatgcgatg
    15961 ctgacgcatc ttatccgccc taccatccct cccggcaaca tttattgccg cttttgttta
    16021 catattctgc cgctaaacaa ttccccattc ctggcgtata tctggctaac attcatcaat
    16081 gtgatagatt cctctcccgc atttatggga atgcgtagtg acttattcta attattttta
    16141 taaaagcatc cgtgataatg aaaaggcaaa gaaacgtcaa tttgttattg atgttggtat
    16201 tactcgtggc cgtcggtcag atggcgcaaa ccatttatat tccagctatt gccgatatgg
    16261 cgcgcgatct caacgtccgt gaaggggcgg tgcagagcgt aatgggcgct tatctgctga
    16321 cttacggtgt ctcacagctg ttttatggcc cgatatccga ccgtgtcggt cgccgaccag
    16381 tgatcctcgt cggaatgtcc atttttatgc tggcaacgct ggtcgcggtc acgacctcca
    16441 gtttgacggt attgattgcc gccagcgcga tgcaggggat gggcaccggc gttggcggcg
    16501 taatggcgcg tactttgccg cgtgatttat ataaacggac acagttgcgc catgctaaca
    16561 gcctgttaaa catggggatt ctcgtcagtc cgttgctcgc accgctaatc ggcggtctgc
    16621 tggatacgat gtggaactgg cgcgcctgtt atctcttttt gttggttctt tgtgctggtg
    16681 tgaccttcag tatggcccgc tggatgccgg aaacgcgtcc ggtcgatgca ccgcgcacgc
    16741 gcctgcttac cagttataaa acgcttttcg gtaacagcgg ttttaactgt tatttgctga
    16801 tgctgattgg cggtctggcc gggattgctg cctttgaagc ctgctccggc gtgctgatgg
    16861 gcgcggtgtt agggctgagc agtatgacgg tcagtatttt gtttattctg ccgattccgg
    16921 cggcattttt tggggcatgg tttgccgggc gtcctaataa acgcttctca acgttgatgt
    16981 ggcagtcggt tatctgctgc ctgctggctg gcttgctgat gtggatcccc gactggtttg
    17041 gcgtgatgaa tgtctggacg ctgctcgttc ccgccgcgct gttctttttc ggtgccggga
    17101 tgctgtttcc gctggcgacc agcggcgcga tggagccgtt ccccttcctg gcgggcacgg
    17161 ctggtgcgct ggtcggcggt ctgcaaaaca ttggttccgg cgtgctggcg tcgctctctg
    17221 cgatgttgcc gcaaaccggt cagggcagcc tggggttgtt gatgacctta atgggattgt
    17281 tgatcgtgct gtgctggctg ccgctggcga cgcggatgtc gcatcagggg cagcccgttt
    17341 aagcgcacgt caccgcagca tcgtcatcag ctccatggga gaacgatgct gctttatcag
    17401 atcacgcatc acccgcatat gcggtgcgga gtaagaataa aacgcctgat agcccgcaca
    17461 aagcacgctg tctggcgtct cctgccgagc gtgcgggcac aggcgatgcc agtcgcacgc
    17521 ctggcagtca cggctcggtt gcgggttttc gctgccacac catttttgta aggtggcgtc
    17581 aaaaagctca atcgagatac gactgatatc tgcccgcacc caacactcaa acagggcaat
    17641 taaaaatttc ccccacagct ctgctgtcac cgaagcggcc gtcagatcgc cccggtcatc
    17701 aaattcaaga tggggaatga gctgtacgga gcggatgccc cgctgtttga aattgtgata
    17761 aaaatcggca ggttgcagag catccttgta ctctacaacc agcttatgcc tgtcctcttc
    17821 agcatcaatg acctgacttc ctgtcatgcg actctctcaa taaatacgat aagttgatgt
    17881 atatgcgtta cagcaaacag tattgcgaga gataacaccg cgagggagat caaaaatttg
    17941 tcacggacta cgtgaaattg ggaaaaatcg ctattcgtga catccattaa attgcgatta
    18001 cgggtgtagt gccagaggat cagcgccacg atggcgagga tgccaatcga aatccagaat
    18061 aacatgcccg cctggtgcca gttgtgtttg atagccaacg ccatcaacgc gccgtagccc
    18121 agcatggtac gaaaccacgc cagtgatgtg cgctccggct gcagccctgg gtcggcaatg
    18181 cgtctggctt tacggctatc cggcatacaa caccagtccc ataacgatca ccgccacaac
    18241 catcaatatt aagctgatga ttaataagct gttggtgtag ggcaggtcct ctttcagacg
    18301 catcgctttt tcattgcgta accagcgcag gtagccatac attgctaacc cgccggaaaa
    18361 cagacacaac aacaacgcca gcagttcacg aatgacgggc gtggcgaaat ctggcgcaag
    18421 ttgatcaagc ccaacccccg ccgccagaaa tcctagtgcg gtacggatcc acgccagaaa
    18481 agtacgctca ttagccagcg agaagcggta atcgggcgct tctccaaggc gggaaatttt
    18541 cattgttgct cctttgcaat ttcactaacg ctctatcttt agcggttttt tttaagaatt
    18601 tcgtccgccc gtttcctgat atgcttttgc catccgctac aaggaggtca gatgggtatc
    18661 atagcgcaaa ataaaatcag ttcattgggc atgctgttcg gtgccatcgc tttaatgatg
    18721 gggattatcc acttttcatt cggccctttt tccgcaccac cgccaacgct tgaaagcatc
    18781 gtggcggaca aaacggctga aattaaaaga ggtcttctcg ccggaataaa aggcgaaaaa
    18841 attaccactg tggagaaaaa agaggacatg gatatcgata aaatcctcga tcaaagcggt
    18901 atcgcactgg ccattgctgc gctactttgc gcatttattg gcggtatgcg taaggaaaat
    18961 cgctggggca ttcgcggcgc gctggttttt ggtatcggca tcgtgtgcag cattttgctg
    19021 atattcctga ttttttcctt cctgaccggc ggttcgctgg tttaagcagc ctgaataatg
    19081 cccgccgggt gatcatcagc gggcattatc gtcagaattt ctgttttttc tcctccacct
    19141 tcactccttg cgtcggtagg ccggtgtcat agtcgcgtac cactggcgaa tagccatctt
    19201 gtgggcgtgg gcgaaacgcc cccatccagc gcggtcgtgc atctttacgc cacggacgca
    19261 gactccattg gtaactgcgg aacggatcgc gaattttgtc catgtaatcc aataaggcgt
    19321 catgcatttt gctgcgaacg tctgcaaaac ggatatcatc gatcaggtta tgcatttcat
    19381 ttgggtcatt acggcgatcg taaagttcat cactggtgaa gaggttgagc accagtttaa
    19441 agtcatccgt cacccagcaa cgcaccggaa taaaaccgcc aaagctgtca tgctcaatct
    19501 cgtagcggtt aaattccacc atcacgccgc gtggctcttt cacggcaagg atattttccc
    19561 ccggcagaat ctctggtttt tcaatatctg ccagcgccat cattgtcggc agtaaatcaa
    19621 tatgactgac tggcgtatcg acctgtcgcc gctccccttg cggcgaacgg atgatcagcg
    19681 gaatacgggt gatgtcgtca tacatcgccg ctcctttact gatcagctta tgtgcgccca
    19741 tcatttcacc gtgatcggag gtataaatga cccacgtatt ttcacgttgc tctggcgtta
    19801 aggcattgat gacacgtccg atttggtcat caacaaagtc attgcaggca aaatagagcg
    19861 gatggtgata aagcccgtca tcaccgactg gcgatagcat cgcctgcgcc cataagcggt
    19921 gatgttccgg tttgttcgcc aggtcatcct gtgatttctc gccaagttcg tagtaaaaat
    19981 cagcgtattt ctctaaatac tccaccggac aggtgaacgg gtgattcggc tcatcatacg
    20041 aaaccaccat caggaagggt tcctcggcgc gcacgggctg ttgcagaaaa tctaccgctc
    20101 gattgctgat gcggtgcgcc caggtgaagg tttcgtcaat atggttcgcc tgtaaatctt
    20161 cgacgctgtt taggccattg cgccacaggc tgatctcttt ttccgtcagt tcgctaagat
    20221 agttcgcccc atcaaaccag taatcagcat cccactccgg cggacactcg ccagtgccga
    20281 aatagtcatg accgtcgaga tgccatttgc cgatgtaaca ggtgtgatag ccggcatcct
    20341 taaagtagcg ccccatagtg gagatgtttt tgcctggcgc gacgttgttg gtccacgggc
    20401 cggactggtt agcgtagata ccagtgaata gtccggcgcg tgcaggcgta caaaccggtg
    20461 aacaagtgta ggcggaatta aagcgaatac cttccgccgc cagactatca atattttgcg
    20521 tattcagcgg tttaccgcta tagcaaccga ccatattggt ggcctgggta tcggtcaatt
    20581 ggggcgtttc ataggctatc cttcgcgtcg ggagtaaagg ttacggctgg gtcgtgacga
    20641 tgccgccagc tgtcaaaaat caggtagaca atcactacgg cagcgatgaa ataactgatc
    20701 atcgctaacc agcgcgtacc gtagccgccg aattcagcca acccgacata gacgccaatc
    20761 atcgcgaaca ggataccaat tgacgcgatc ttgacgtttt tccacggttt catgtccacc
    20821 gcaaacgcat ctttgaaggt gaacggcgtg gcgcgcgctt tgataaaacc gatcaccagc
    20881 atcacgacca cgttgatgca gaacgtacag gccagcacat agaggaaatg gaagtcgaac
    20941 ttcaccagat agttgatggt gatgtagctg attatgccaa tccccatcgc cacttttgcc
    21001 gccagcgccg ggatgcgcgg gaagaaaaag cccataatga tgatggtaac cagcggcacg
    21061 ttgtaaatgc cgttgagctg tttcatccag ctatacagcc cctgcggcgc gttggcgatc
    21121 cacggcgcta ccaaaaccga aacgatagcg ataaagaaac caaatttgcg cccgacggtg
    21181 accagctgct gcggctcggc attctggtta atgatgcgac ggtaaatacc catactgaat
    21241 aatgtactgg cgctattcag aaagccgttg aaggtactga tcaccgcacc aaataacacc
    21301 gcgccgaaga aacccaccag tggcactggc agaacgttat tgaccagcgt cgggtaggcc
    21361 atgtctgctt tcggtagatc ctgatacaga tgaaacgcaa tcaaccctgg cagcaccagt
    21421 accagcgggt cgagcatttt cagcaccgcc gttaacagcg cccccttttg cccttccgcc
    21481 aggctttttg acgccagcgt gcgttgcacg atgccctgat ttgtacacca gtaaaaggtg
    21541 ttcaccagaa ttaaaccggt aaatgccgcg ccaatcggca agggatcggt cgggccaccg
    21601 actgagttta atttctcggc gtgaacggtg gtaatttgct caatgccctg cataaagctg
    21661 cccttgccca tcgcgatcag gccaaatacc ggcaccatca acccgccgat aaccagccca
    21721 ataccgttga tggagtccgc cactgccatt gcgcgcagtc cgccgatcac cgcatacaaa
    21781 attcccgcca gaccaagcaa aattaccagt agccagatag ccgcaccgtg agaaatctgt
    21841 agcgattccc cgacgtgaaa caggctgttg agcgccaacg cgccggagta gagaacaatc
    21901 ggcagaaagc agacgccggt ggcaatgagg aagcagaagt cgatgataat acgcgtcgtt
    21961 ttatcataac gttcctccag aaaatcgggg atggtggcaa tgccgcgctt cagatagcgc
    22021 ggtaaaaaga ttagcgcgag gaagatcagc gtcaccgctg aagtcacttc ccagcccatc
    22081 accgacatgc cgcttttgta ggcctgcccg gaaagaccaa ccagttgttc cgtggaaagg
    22141 ttggttaaca ttaacgaagc ggcaataacc ggcgctttta gtgaacgtcc ggcaagaaaa
    22201 tagccttgtt gcgatccggt atctgttttg cgaaccttcc actaggtgat caccgccacc
    22261 agcagcgtaa aaccgacaaa actcaagatt tgtaacgaat tcatctcata gcccttattt
    22321 atgtttatgt cccggacgca tgaatatagg gagaggtaaa atgtgtcggg tcaggcagtc
    22381 agattcttaa aatacaaacg tcgtatccct gaacggattg ttttctgtta agttcaggtt
    22441 gtgagcataa ttcgccggag ggatattaaa atgaatggaa aattgcaaag ttcagatgta
    22501 aaaaacgaaa ctccgtacaa tattccctta ttgattaacg aaaatgtgat ctccagcgga
    22561 atttctctga tctcgctctg gcatacctac gccgacgagc attaccgggt gatctggccg
    22621 cgggacaaga agaaaccgct tatcgccaac tcatgggttg cggtgtatac cgtacaagga
    22681 tgtgggaaaa ttcttttaaa gaatggcgaa caaataactc tgcatggcaa ctgtattata
    22741 tttttaaagc caatggatat tcactcttat cactgtgaag gtttagtctt ggaacagtac
    22801 tggatggaat ttacccccac cagtatgatg gatattcccg ttggtcagca aagcgttatt
    22861 tataatggcg aaatttataa tcaggaactc accgaagttg ctgagttaat aacttcacca
    22921 gaagcaataa aaaataatct ggcagtcgct tttctgacga aaattattta tcagtggatt
    22981 tgtcttatgt ccgcagatgg taaaaaagat ccacaacggc ggcaaattga aaaattaatt
    23041 gccactttac atgccagtct gcaacaacgc tggagcgtag ctgatatggc tgccacgatc
    23101 ccctgtagcg aagcctggtt gcgtcgtctg tttttacgct ataccggcaa gacgccgaaa
    23161 gaatattacc tcgatgcgcg tctggatctg gcgctatcgc tattaaagca acaaggaaac
    23221 tcggttggcg aagtcgctga tacgctcaac ttcttcgact cctttcattt cagcaaagcc
    23281 tttaaacata aatttggtta tgcgccgtca gccgtgctaa agaatacgga ccagcaccca
    23341 acggatgcca gtccacacaa ttaacgtaac tctggccagt acgctttgtt ggcctccagc
    23401 aactcatcca gaatagcttt ggcgaccgag gcgctcggta ccgttttcga caacgtaatc
    23461 gcctgccaca ggtgctgata tgaacgctgt tcccaggcgt ccacgaccag tttttccacc
    23521 gccacttgct gactcattaa ccctttctga aactgcggga tatcgccgac caccagtggt
    23581 tcaggccgta gtagcgcacg cgcatctgtt tgcgatcttg cagcccaaca atttgcgcca
    23641 tccggctttc aataccgatt ggcatgtcac agatgttgag gattttcgca ttcgggcgca
    23701 gacgacgcgt ggcttctgcg acaattgctg ccgggttgga gtagttgagc atctaggcgt
    23761 ttggtgaata tttttccata taatccacca gttccaggac gccgccaatg gaacgcatgc
    23821 cgtacgctat tccgccgggt ccgcaagttt cctgaccaac aacaccgtgg cgcagtggga
    23881 ttttttcatc cagttcgcgc atcgggtact tgccgacgcg gatgtgcgcc atgacaaaat
    23941 caacgtcgct gaacgccact tcaggatcgg tggtgtaact aaacgcaatg tccggcgctt
    24001 tttctttaag gatgacttta caggcttcgg caatcacttc ctgccgcgcg ccatcgttat
    24061 cataaaattt cagtgcacga agcgggaaac ggtcctgatt cgccaggagc atcaacacga
    24121 tgcctggcgt aaaggtgctt ccaccgcctg cgacaaccac tgagaatttg gtcattatac
    24181 tgcctccgta atggcaacat tttctgctga ttgatgagaa ttaattaagc tatcgagctg
    24241 ttcacgcaat tgggatacat gcagaccaat gatcacctga atggcatcgc cactacggaa
    24301 gacgccgtgc gctcccagct ttttaaagac ttcgtcatcc agcgtttgtg acatgtcatg
    24361 cagtgcaata cgtagacgcg tcgcgcaatt gttaatgctg gagatattgc cgaccccgcc
    24421 cagggcttgc aggataccgg cagcctgatc cagctctttt tttggctctg cagcggtggt
    24481 ttggcctcgc gaggctttgt attcggcttt tgagtagagt ttcacttccg catcttcacg
    24541 tcccggcgtg cacatgttga actgcagaat cagggtgcgg aaaaccacga agtacagcag
    24601 ggtaaagcac aacccaatgg cgatttgggt cagcatcata tccgcgtggt tgctgaacat
    24661 cgggatccag ttttgcggta aaacctggtc aatcagacct ccgcccatgt tgcccaccac
    24721 accaaagaga tacattacgg tcgacattga ggccgccagc acggcgtgta ccgcaaacag
    24781 caacggtgaa atgaacagga aggtaaattc cagcggttcg gtaattccca ccagcatggc
    24841 ggttaaggtt gcgggaatca gcaagcccgc cacttttacc cgattttccg gtgcggcagt
    24901 gaagtacatc gctaaagaaa tgcccacggc accaaagatt tttgagttac cgtgcagggc
    24961 aaaaccgcct tccgggaaca acgatttcag cggctcggcg ctcagactga actcttgcag
    25021 atgctgcgcc cagtacatct gaatgccgcc ttcaacagct gccggaccaa agataaacgg
    25081 tccgtagatg aagtggtgta aaccggttgg gatcagaata cgttcgagga aggtgtaaac
    25141 ccacacccca agcgcacccg ccgaacgcag gaacgcttgc agagattcaa tccccatttg
    25201 tacttttggc cagccgagca atgtcagcca ggcacagggg atcatcacca ggaaggcgat
    25261 aatcaccaca taagacgtcc cctggaaaat gccgagaaaa accggcagtt ttttatcgaa
    25321 cagacggtta tgcagcgccg tcacaatgcc ggaaatgata attgcgccga taatgctggt
    25381 atcgagggtt ttaatcccgg ccatcattgt cagaccacta cctgccaccg cgtcctgagt
    25441 gaaatcgacg ccgaagtagc ttccccaggt cattcccatc gcattgatga aatagttcca
    25501 ggtcaggaaa ctcaccatca ccgccagaca agcacgtccc tgcgcttgct tagcaaggcc
    25561 aatgggtaaa ccgacagcaa aaatcagcgg catattacgg aataccgtcc aaccgccctc
    25621 ttcaataatg tgtacgattt gcgcgaataa actgttcgga tcggtcagtg attccccgac
    25681 aaacatcggg ttttgcagca agatggcaag acccaccaca atcccggcga agggaaacag
    25741 cagcactggc gtgaacatcg cgccgccaaa gcgttgaatt tgactgagca tttccacatc
    25801 ctcttttctc aattctgtaa gtaggaagcc tgattggtta tgcggcagac ctggagcgag
    25861 gataggaaga tgcgatcggc agaacatgac gacccaatgg gattcgtgat cgctttcatg
    25921 cttttcgttg ggcggtagag atctacgttt tcgcgaaaaa gtagacatct gtgagcacag
    25981 gtacacagca tcgaacacat ctttaaaaaa aagatttttt tcaatcgatt aagcagaact
    26041 tgtgggcgca ttacccgtgc ttgcaggcaa aaaagagagc tagagatgat ctacaaaagc
    26101 attgcggagc ggttaagaat tcgacttaat tccgcggatt tcacgctaaa cggccttctt
    26161 cccggtgaaa aaaagctggc ggaagagttt gcggtatcgc ggatgactat ccgtaaagcc
    26221 attgacctgc tggtagcgtg ggggctggtg gtccgccgcc acggcagcgg cacttacctg
    26281 gtgcgcaaag atgtgctgca tcaaaccgcc agcctgaccg gactggtgga ggtgttaaaa
    26341 cggcagggaa aaacggtcac cagccaggtg ctgatttttg aaatcatgcc tgcgcctccg
    26401 gccattgcca gccagttacg gattcaaatc aacgagcaga tctacttctc ccgtcgcgtt
    26461 cgttttgtgg aagggaaacc gctaatgctg gaagacagct atatgccggt aaaactgttc
    26521 cgtaaccttt cactgcaaca tctggaaggg tcgaagtttg aatatattga acaagagtgc
    26581 gggattttga ttggcggtaa ttatgaaagc ctgatgccgg tgctcgccga tagactgctg
    26641 gcgcggcaaa tgaaggtagc ggaacacacg ccactgctgc ggatcacctc gttgtcatat
    26701 agcgagagcg gggagttttt gaattattca gtgatgttca gaaatgccag cgaatactag
    26761 gtggagtacc atttacggcg accccacccg gaaaagagtt aaccgatact ccagaagagc
    26821 accgccagta attggggggt gataatgcgc aggaacatca ctaacggata gacagtggcg
    26881 taagagagcg ccgccgcacc actggttgga tgaagattat tagcaaacgc cagcgccgga
    26941 ggatcggtca tggaacctgc cagcatcccg cacatggtca ggtaattcat tttggctaac
    27001 atccgcgcca gaatgccaac agtaatcagc ggaacggcgg tgatcagggc accataacca
    27061 atccagctta gcccttcgcc attgaccagg gtattcacaa aatccccacc agatttcaga
    27121 ccaacgaccg agaggaacag cacgatcccc agctcccgca gcgcgaggtt ggcacttggc
    27181 ggcataaacc agtacagctt gccgatactg ccgatacgcc cgaggatcaa cgccataatc
    27241 agcggaccgc ccgccagccc cagtttcaac gcggccggga atcctggcac aaagacggga
    27301 atagaaccta acaatacgcc aagcccgatg ccaataaaca ctggcagcat ctgaacctgt
    27361 tgcagttttt gctgcgcatt ccccagcaca ttggcaacgg catcaattgc ggacggacgc
    27421 cccaccaggt tgaggatatc gccgaactgc aggctgatat cgccactggc gaccagttcg
    27481 accccggcac ggttcaggcg cgagataaca acgtcatagc gttctttaaa gtgcaggtcg
    27541 cgaatacgtt ttccgagcac gttttcattg gtgaccacca cacgctcgac gcgcaaatca
    27601 gtgcctttcg tagacagcga ggtatcgacc tcctgaccaa tcaccagttg cgcattatgt
    27661 aaatccgctg gctgccccac cagatgcagc aaatcgccca gttggataat ggtatctggc
    27721 gaaggtactt ttagggtttc ttctcgtttc agacgcgagc agataatttt gtcgccgttg
    27781 agaatcggta catctttaat ggctaaatca tgcaggttag ggttctcaac gcgaatattg
    27841 atagtcttga tcagcgcgcc gccattggtg cgtgaagact cgtgctgctg agcttctgtc
    27901 tcgacattga cgcggaaaat aacccgcaac atccacatgg tgaacaaaat cccgcaaatg
    27961 ccgaatggat acgccatcgc gtaactcatc cccatctgat cgaccatttc cattggtgta
    28021 cccaggtcgc gcaagatctg ctgccctgcc cccagcgctg gcgtattggt taccgcaccg
    28081 gagaaaatcc ccagcactac cggcagtgga atatcaaaca gtttatgcag gatggcggta
    28141 accagaccac cgatgatgac gatcagaaca gcaaacaggt tgaggcgtaa tccggagacg
    28201 cgcaatgagg caaagaagcc cggccctacc tgaatcccga tggtataaac gaacaggatc
    28261 aggccaaatt cctgaataac atgcagcata tcgctactta atgtcatccc cgcctgagaa
    28321 acaaaatggc cgacgatgat cccaccaaac agcacgccgc caatacctaa tcctatgccg
    28381 cgaaatttga cgttgccgat aaacaaaccg acgactgcca ccaaagccag aatactgacc
    28441 gttaatgcta tatcactcat cgtccatttc tcttgagata acattttaat tgctacggat
    28501 tatggctgaa tcggtgaagc agatttgcct tatagcgcac attatcacgg gaaagaacgt
    28561 gccgaaatat cttaaacagt cgaaatttaa cgcattgttg aagaagaaag acaaaaaaag
    28621 ccccaccaga atggcggggc aaagagaata gctagttagc tatttaacgc gggacgttcg
    28681 ctgatagcga tacgctgcgc tgcgatgggt tcaggctcat tacgaattaa atcaatatgc
    28741 agtaaaccgt ttacgaaggt tgcgccagag acttccatat tttcagccag cgtaaaactc
    28801 aggctaaatg gctgattcat aagcccttga tgcagccatt ttttctcttc ttttggctgc
    28861 tccggcgtgc cttttacgct caggcgcgta ccttccagtt gaatctctaa atcttcctga
    28921 cggaaacctg ccagcgcaag ggtaatgcgg tagtggttat cgtcgctttt ctcaatgttg
    28981 tacggcggga agctctggct ttcaccggcg ttttgcagtg cgttggccag tttgtcaaaa
    29041 ccgatccatt gacgcatcag tggggataaa tcgaagttac gcatagtcat ttctccttct
    29101 aagaagcgag taagtacctg caaatccgaa gattcgcata tgctccctga cggcgagcat
    29161 ggagatgtca ggccgcgcca ggcggcctta gggaattagt tgatttcgat acggcgcggt
    29221 tttttcgctt ccggaatcac gcgttcgaga tcgatataca gcaaaccatt taccaggtta
    29281 gccccacgaa catgaatgtt ctcagctaac tggaatttgc gttcaaagtt gcgttcagcg
    29341 atgccctggt acagataggt gcgctctttt tgttcgtcgg cgtgagcacc tttcaccacc
    29401 agcagattat cctgggcggt aatttccagt tcgctctcag caaaaccagc cacagcgata
    29461 gcaatgcggt aatggttttc gtctaccagt tcaacgttat acggagggta gccgccatta
    29521 ctctggctct ggttgttttc taagtggtta aacaaacggt caaatccaat agcagaacgg
    29581 taaagcgggg ataaatcaaa gttacgcata atcaatagct cctgaaatca gcgagaatgt
    29641 aagaccttcc acaatggaca ggtcaggtag ccagaacacc catcaggcgc gtcctcatcg
    29701 actacgatgt aaaatgggtc tggaaatgaa cttttcaagc cttatcagcg gacttttttt
    29761 tgcagtttat ggtctattgc atagactgag ggggcagcac gaaacgttaa agtgcgatag
    29821 ccgccacagt gcgacgtaat gatgatgtta ttttcagcaa gggatcgcta taactcatca
    29881 ggccaacacc cacgatcaac agatgaattt attatgataa gaaatgtgtt gttagcgttc
    29941 atgatatgca gcggaatgac attactcgga ggatgctcca gcgtgatgtc ccataccggg
    30001 gggaaagaag gaacgtatcc cggcacccgc gccagcgcca ccatgattgg cgacgatgaa
    30061 accaactggg gcactaagtc gctggcgatt ctcgatatgc cgtttaccgc tgtgatggac
    30121 acgcttttac tgccgtggga tgtgttccgc aaagacagct cggtgcgctc gcgcgttgaa
    30181 aaaagcgaag ccaacgcgca agccaccaat gcggttattc caccggccag aatgcctgat
    30241 aactgattta gcgatcggtt tcggtgatcc acagctggcc accttccatc cacgccagcc
    30301 attgaccatc cggcgagaag accacggcgt ccgcagaagg tggattggcg tggttttcgg
    30361 ttaaatactc aacctcgccg ctttgcgcat gggcacaaac aattcgatta tccagcacaa
    30421 agcccaacca ttctcctgac ggatgccagt taaatgcaga ctgaatatcc gttttgttat
    30481 gggttaactt gcgcggttcg ccgccctgtg gcgagataag ccacagttgc acaatgccgt
    30541 tatcatcacg cattaaaaac gcgatttgcg taccctgcgg attacagcgc acccagtggc
    30601 gcgggtaggg ttgtttccgt tccacttaac ggcgcatcac ctgccgattt ccagctagct
    30661 tcatcttgcg gtaactcaac gataaatagc tccggcactt tctcgccctt tggcgaaagt
    30721 gtgtcgccaa taaacgccag cgcgtgattt cctacccatc cttcttcata agcacgattg
    30781 atttcatcac tgccaggctg cggcgtgggc gtggttttac tcaccagcac acaccagtgg
    30841 ctaccgctgt attcacgcgg atgttgcttt tgtacgttga ccgggccaaa cggtgcagca
    30901 accccgacgt ttcgcaaatc cagcgccgga tcgagttgat gcattacatg gtcgttatag
    30961 gtaaagctcc ccctttcacc gttcgggcta aagacatgca catggctgcc accgcgcagc
    31021 gctcctggcg tatacggcgc ggtaatatcc attgcatcga gattgctcac cttgccgcct
    31081 tcagcaatga ctccgcgtcg atgatggaag tcgtaatgcc atgtttcatc aggattttca
    31141 gggccgtgaa taaagacata tttctctgac ttcggatgaa cggtcaccac gccgacgtgt
    31201 gcgccctgtg acgcgcgata gataacctcg acctcgcctg tatggatatt cacacgctca
    31261 atggtctcgc cggtaaacga cgccccagaa ggacgcacgt caaataccag ccactggctg
    31321 tcgggcgtcc aggtattggt attggtgagc aggtgattac ggggagcaaa ggttatctgt
    31381 ttcatggcgc ggtcctgata caaaacggtt cccgccatca tacttcacaa ggacttcact
    31441 ttcagacaat agtcttttgc catcgtgatg aatgtgagtg aaaagatgga acatttcgac
    31501 gtggcgatta ttggcctcgg cccggcaggg tcggcgttgg cacgaaagtt agccggcaaa
    31561 atgcaggtga tcgcgctgga taaaaagcac cagtgcggta ctgaaggttt cagcaaacct
    31621 tgcagatttt cagcgtcaaa actgtcgacg tcgccgcatc gctcacgcgt aactaccagc
    31681 gaagctatat caatattaat cgccatgctt tcgacttgtg gatgaaatcg ctgatccccg
    31741 ccagcgttga ggtttatcac gatagcctgt gccgaaaaat ctggcgtgag gatgataaat
    31801 ggcatgtcat ttttcgtgca gacggctggg agcaacatat taccgcccgc tatctggtcg
    31861 gtgccgatgg cgcaaactcg atggtgcggc gacatctcta cccggatcat caaatccgta
    31921 aatatgtcgc tatccagcag tggttcgcag agaaacatcc ggtgccgttc tactcctgca
    31981 tctttgataa tgcgataact gactgttact catggagtat cagcaaagat ggttatttta
    32041 tctttggcgg tgcctatcca atgaaagacg gtcagacgcg tttcacgacg ctgaaagaga
    32101 aaatgagcgc ctttcagttc cagtttggca aagcggtgaa aagtgagaaa tgcacggtgt
    32161 tgtttccctc acgctggcag gattttgtct gtggtaagga caacgccttt ctgattggtg
    32221 aagcggcggg atttatcagc gccagctcgc tggaggggat tagctatgcg ctggatagcg
    32281 cagagattct gcgtgcggtg ttcctgaaac agccggagaa gagcaacgcc gcttactggc
    32341 gcgccacacg caaactgcgt ttaaaactct tcggcaagat agtaaaaagc cgatgcctga
    32401 ccgcaccggc tttaagaaag tggattatgc gcagtggtgt ggcgcatatt ccacagttga
    32461 aagattatcc aacgcgcttc acatcaccca ccagcaggat gtaagagagc gcgccaatca
    32521 acgcgacggc ggagatataa accagtgcag gcgcgaaacc gtaaccctgc gccaggtacc
    32581 ccaccaccag cggaacggtg atgccgccca gaccaccggc gaagttaaac acgccgccgg
    32641 ttaaaccaat caggcgcatc ggtgccagag aagagaccag cgaccaggta atcgaagcaa
    32701 aaccgttgcc gaagaatgcc agcgccatca ggcacataat catcatcgga tcgttggtgt
    32761 agttagcgcc cataatgcag gtggagatca gcaagccgca gataatcggc gttttacgcg
    32821 caaagcccag tgaaaagccc ttacgtacca gcagatccgc gacccagcca gagagcagga
    32881 cgccgacaaa cgccgcgagg aatggcaccg tggtcataaa gcctgctttc agcgccgtga
    32941 ttcctttttc ctgggttaaa tagttcggga accaggttaa gaaaaaccac agtgcagaag
    33001 ccaccgcaaa ttgcccaaga taaacgccga tcagtttacg atggaacacc agtttccagt
    33061 ctttggctgt taacggctga cgcgcctctt tcttcaccgg cgcatcacca tccaccagac
    33121 cgccgccatc acgaatgtaa tccagttcag ctttgctgat acctttggtc aggcgcggcg
    33181 gctgataaac cttaaaccaa atcagcgacc agataatgcc gataccacca gtgacaatga
    33241 acacccagtg ccagctcaac atctcctgaa tccagatcag cagcggagtc aaaaacgcca
    33301 gaccgacaaa ctgaccagac gtataaaaac caacggcgga agcgcgttca tgttccggaa
    33361 accagctggt caccatccgg ttattggtcg ggaaagcggg cgcttcgaaa ataccggtta
    33421 tcgcacgcag accaattaat gacattaatc ccgtggcaaa gccctggaat aaagtcgcca
    33481 ctgaccaacc aaatatcgca ataaaataag tcacgcgaga acctacgcga tctaaaaacc
    33541 aaccgccggg gatctggcat agcgtataaa gccaggcgaa ggccgaaaat acatagccca
    33601 tttccgcttt cgtaatgccg aactcttcct gaatatgggc ggaagccacg gccaggttag
    33661 cgcggtcaac ataacaaatg accaccgtaa taaagatcat caccagcgtc agataacgcc
    33721 gacgccccgg ctttgctgca ttaacgggaa tatccatagc gagctttctc cagattttgg
    33781 gcatagcgaa gccgctcacc atgccctgta atttacagag ggttatattt ttgtattgct
    33841 gttttagtgc ccgatgaggg gcttacgtgg caggaattac cactctgcta cgctgttatc
    33901 ttcatgacgc cagagcggat tacgccagtc cggggcattt ttactgaact caataacttt
    33961 agcttcgtcg atttccacac ctaagcccgg tttcgttaac ggtttaaaga agccgccaac
    34021 catgctgaag tcttctttgt ttttcacaaa gtcgagtaac tccgcgcctt tgttgtaatg
    34081 aatgcccata ctttgttact gaagtaccgc gttataggaa acaaagtcga tatgcaggca
    34141 agccgccagt gcaatcggtc cgaacggaca gtgcggcgca agggtcacat cataggcttc
    34201 tgccattcca gcaattttgt agcattcggt aataccgcct gcatgggaga gatccggttg
    34261 cagaatcgaa ataccgcctg cctccagcac gcgtttaaaa tcgaaacgtg agaacatgcg
    34321 ctcacccgcc gccagtggaa tatgcgtttg tgccgccaat ttcgggtagt attcggcttg
    34381 ttccgccagc accggctctt caataaacag cgggcgatac ggctccagtt ctttaatcag
    34441 cactttcgcc attggcgcgc tgacgcgacc gtggaaatca agaccaaact caatctgatt
    34501 gccaaaagct tcacgaattt gtgccacggt gttgactgcc gcatctaccg cgcgggagtt
    34561 atcaattagc cccagttctt cacaaccgtt cagtttgaag gtatcgaagc cgatttcgcg
    34621 cagggtttta atgccgtcga taacatccgc cggacgatcg ccgccgaccc aactgtaggc
    34681 tttaattttg tcgcgaacca ggccgcccat cagttgccag actggcgcat tcagcacttt
    34741 gcctttgata tcccataacg cctggtcgat cccggcgatg gcgctcatca ggattggacc
    34801 gccacgataa aatccggcgc gatacatcac ttgccataag tcgttgatgc gcgaaggatc
    34861 ctgaccaatc aaatagtcac ccagctcgtg aactgccgct tccaccgtac gggcgcggcc
    34921 ttcaatcacg ggctcgcccc aaccgaccac gccttcatcg gtttcaattt tcaggaacat
    34981 ccagcgggga ggtaaacgat acgtggtaat tttggtgatt ttcattgcac tgcctctcga
    35041 tacgccttaa caaatgctgc tgcctgctgc gcggtgcgtt ctacggactg cccggcgcga
    35101 taaagatcgc tgcctaagcc cgccccaaca caacctgcgt ctatccactg cgccaggttt
    35161 tctggcgtca cgccgccaac ggcaaagact gcgatgtccg gtggtaatac cgcttttaac
    35221 gctttgatgt attgcggacc aaaagccgat gacggaaata ttttcagcgc ctgcgcgccc
    35281 gcttcgagcg cggtaaaggc ttcggtcgcc gtcgcgcagc cggggcaaac ggtcatgccg
    35341 tagcccaccg cgcggcggat cacttcacta tggatattgg gcgtaacgat gagctgacag
    35401 cccatcctgg cgagcgcatc gacctgttca ggtttcagta ccgtacctgc gccaatcaac
    35461 gccttgtcgc catacgcatc aacgatggcg ggaatgctct gctcccattg tggggaattc
    35521 agcgggattt caaccgcgtc gaacccggcg tcaatcaccg cgccaacatg cgccagcgcc
    35581 tcgtcgggcg taatgccgcg caaaatggcg atcagcggga gtttagtttg ccactgcatg
    35641 agcgatgctc cttataccag cctgaaatgc cgtgtcgccc gccaccgccg ccacgtcgca
    35701 acccatcgcc tgaaaggctt gctggtagcg cgcggtcagc gatgttccgg cgacaagggt
    35761 gatggcgtgt tgatgggtca catagtcgcg catactggca acctctgcgc caatcaacaa
    35821 accagagaga atgttcgcgg agaagtgttc ccagcacatg cgaggcgcga acttcaaaaa
    35881 gctgcggcaa tatggcgggc gcattaaggc cgcgctcaag gccagccgcg aaggcatcgg
    35941 cagagttttc ctgcggcggc aaacctgcgc caatcaatga gtgatttaac agtaaatgat
    36001 gtaattcacc ggtcatcacg gtgcgaaaat cgttgatttg ctggctatcg gcctgcaccc
    36061 atttgcaatg cgtaccgggc ataacataaa gagaggaagg agccagagct cgcgcgccga
    36121 tcaattgtgt ttcttcgccg cgcatcacat tgtggttatc gtcatgagag acgcataatc
    36181 cgggaataat ccagatattg tcgccaacag acgttaattg ttcgccaata gacgaaaaac
    36241 gggcaggaac agataaatac ggagcaactt tccagccgac attgctgccg accattcctg
    36301 ccatcaccac tggcgtattt tcttcacgcc agtcggtcgt gacttctgct aacaccgcag
    36361 ccggagattt tccgttcagg cgcgtgacgc ctgcttctga ttgcctgctc tccaggcagt
    36421 ggtcgccctg ataaagccag gcgcgcagat tggtcgatcc ccagtcaatt gcgatgtagc
    36481 gagctgtcat gtgatttcct ttaaccttcg tgtcgagctg gcaatcatgg taagcgccgc
    36541 ctgctctgcc gcatcgccgt cctgatgccg tatcgcatcg aacagcgcct tatgttcctg
    36601 gagcgtttgc ggcatgttgg cctcatcgcc catccaggtt cgttcaaaaa ccgcccgctg
    36661 cagcgaactg atcgcaatgc taagttgctg taacaccggg ttatgcaccg actgcagcac
    36721 cgcctcgtgg tagcgaatat ccgcttcgtt aaacgcttcg cggtcctgat tgttggcaat
    36781 catctcgttc agcgccgatt caatctgcgc cagatcgctg gaggtcgcgc gctctgctgc
    36841 ccaacgggca atcgccggtt ccaccagatt tcgcacttcg ctcatggcac tgataagccg
    36901 tgggtcgtag tcgttttcca gcacccattg cagtacgtca gtgtcgaggt aattccactg
    36961 gttacgcggt gccacaaacg ccccgcgata acgtttcatt tcaatcagcc gcttcgccat
    37021 cagcgaacgg aacacctcac ggatgatgtt gcgcgaggtt gcaaactctt cacagagctc
    37081 cgcctcagcc ggaagtggcg agccaggtac gtatttgccg tgaacgatct gtttacccag
    37141 cgtaatgaca atgcgatcgg ttttattgag agtcatggag agtccttgtg ctctgtatgt
    37201 tcttctctac tttaccccga tcgatgcata acgcggcaac tttgtagtac cagcgtgatg
    37261 acgttcgcgt ttgccgtgcg tgtaatgtag tacaaactta tattgttgtg ctacaattta
    37321 gatcaaaaaa agaacaatgc ataaaaaaat gacatgcgtc gggcagaaat ctgaaaaggg
    37381 atatcaggcg ctaaacagga gggaaagaag agtatgcttt caacggctta gctactcgtt
    37441 taaaagatta atcatgaagt tgaattttaa gggatttttt aaggctgccg gtttattccc
    37501 actggcgctg atgctttcag gctgtatctc gtatgctctg gtttcccata ccgcaaaggg
    37561 tagttcagga aagtatcaat cgcagtcaga caccatcact gggctatcgc aggcaaaaga
    37621 tagtaatgga acaaaaggct atgtttttgt aggggaatcg ctggattacc ttatcactga
    37681 tggtgccgat gacatcgtta agatgctcaa tgatccagca cttaaccggc acaatattca
    37741 ggttgccgat gacgcaagat ttgttttaaa tgcggggaaa aagaaattta ccggcacaat
    37801 atcgctttac tactactgga ataacgaaga agaaaaggca ctggcaacgc attatggttt
    37861 tgcctgtggt gttcaacact gtaccaggtc actggaaaac ctaaaaggca caatccatga
    37921 gaaaaataaa aacatggatt actcaaaggt gatggcgttc taccatccat ttaaagtgcg
    37981 attttatgaa tactattcac ccagaggcat tccggatggt gtttccgcag cattactgcc
    38041 agtgactgtt acgctggaca tcattactgc accgctgcaa tttctggttg tatatgcagt
    38101 aaaccaataa tcagtaagcg ggcaaacgcg tttatgctgt ttgcccgccc acagattaat
    38161 tcagcacata cttctcaata gcaaacgcca cgccatcttc aaggttggat tttgtgacaa
    38221 agttcgccat ttctttcacc gaaggaatag cgttacccat cgccacaccg acgcctgcat
    38281 attcaatcat tgcgatgtcg ttttcctgat cgccaatcgc catgatttct tccggtttaa
    38341 tacctaacac gtcggccagt gatttcacac ccgtaccttt gttaacgcgt ttatcgagga
    38401 tttcgaggaa gtacggcgca cttttcagca cggtatattt ctctttcact tcctgcggaa
    38461 tacgcgcgat agcctggtcg aggatggcgg gttcatcaat catcatcact ttcaggaact
    38521 gggtattggg gtccattttc tccgcttcgc agaacaccag cggaattgtg gcaacgaagg
    38581 attcatgcac cgtgtagtag ctgatatcgc ggttagcggt gtacagcgtg gtgcggtcca
    38641 gggcgtggaa atgagaaccg acttcgcgag agagtttttc caggaaacga tagtcgtcat
    38701 agctgagagc ggtttgcgcc acggtgctac catcagcggc cttctgtacc agcgcgccgt
    38761 tataagtaat gcagtagtcg cccggctgtt ccatatgcag ctctttcagg tagttgtgca
    38821 cacctgcata cggacgaccc gtcgttagca cgacattcac gccacgggcg cgagctgcgg
    38881 caatcgcatt tttaacggcg ggtgaaatgg tgtgatcggg cagcagaagg gtgccatcca
    38941 tatcgatagc aatgagtttg atagccatga gttcccaagg tagattagtt cctgacccat
    39001 gctaacgcga ttccgctcaa aaatcagtac aacacccgag ggaaaagggg atgcaacgcg
    39061 ggttgcatgc tccccatttg cttagcggaa gagtttccct ttcagcagtt ccatgcctgc
    39121 ggaaagcaga tcgttattgg cttgtgctga cacttcacct tgcggtgaga gcgcatcaat
    39181 aatcttcggc aattgctctg ccagtaaact ggaagccgta ctggtatcca cgccaagttt
    39241 ttgcccgaga tcggacaccg catttgtgcc gagcgccgat tccagttgct cgccgctaac
    39301 tgattgattt ctctgttgat tactcaacca agttgagaga atggccccta agccgccact
    39361 ttgcagtttt tccagcagca cctgaatgcc accctgctcc tcaacccaac ttaaaatcgc
    39421 ctgatatttc cccgcatcgc ctttcagaaa ggcaccgaca acttcatcaa aaagccccat
    39481 gataatcacc tgtaaagcgt tacgtgttga cccaaaaagt atagatctgc gggatgataa
    39541 ttgcggattg cagaaataaa aagggcggag atgatctccg cccttttctt atagcttctt
    39601 gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc atgcaaaatc aatatattgc
    39661 aggagtctcg taggactaat aagcgtagcg catcaggcac gctcgcatgg ttaacgccat
    39721 taaatatcaa tattcgccgc tttcagggcg ttctcttcaa taaacgcacg acgcggttct
    39781 acggcgtcgc ccatcagcgt ggtgaacaac tggtcagcgg caatcgcatc cttaacggta
    39841 acgcgcagca tacgacggct ttccgggtcc atggtggttt cccacagctg ttccgggttc
    39901 atctcgccca gacctttata acgctggatg gagaggccac gacgggactc tttcaccagc
    39961 cagtccagcg cctgctcgaa gctggctacc ggctgacgac gctcgccacg ttcgataaac
    40021 gcatcttctt ccagcaagcc acgcagtttc tcacccagcg tgcagatacg acgatattcg
    40081 ccaccggtga taaactcgtg atccagcgga tagtcagtat ccacaccgtg ggtacgcacg
    40141 cgaacaatcg gctcgaacag gttttgttcg gcattggtgt ggacatcaaa cttccactgg
    40201 ctgccgtgct gttctttgtc gttcagttcg ctgaccagcg cgttcaccca gcgggtaacg
    40261 gtctgctcat cagaaaggtc agcttccgtc aacgtcggct gatagataag ctctttcagc
    40321 attgctttcg gataacgacg ctccatgcgg ttgatcattt tctgcgtcgc gttgtactca
    40381 gataccagtt tctctaacgc ttcgccagcc aatgccggtg cactggcgtt ggtgtgcagc
    40441 gttgcgccgt ccagcgcgat agagatctgg tactgatcca tcgcttcgtc gtctttaatg
    40501 tactgttcct gcttgccttt cttcactttg tacagcggcg gctgagcgat gtagacgtga
    40561 ccgcgttcaa cgatttccgg catctgacga tagaagaagg tcaacagcag cgtacgaatg
    40621 tgcgagccgt cgacgtccgc atcggtcatg atgatgatgc tgtgataacg cagtttgtcc
    40681 gggttgtact cgtcacgacc gataccacag ccaagcgcgg tgataagcgt cgccacttcc
    40741 tgagaagaga gcatcttatc gaagcgcgct ttctcgacgt taaggatttt acccttcagc
    40801 ggcagaatcg cctggttctt gcggttacgc ccctgcttcg cagagccgcc cgcggagtcc
    40861 ccttccacca ggtacagttc ggaaagcgcc ggatcgcgtt cctggcagtc tgccagtttg
    40921 cccggcaggc cagctaagtc gagcgcacct ttacggcggg tcatttcacg cgcgcgacgc
    40981 gccgcttcac gggcacgggc agcatcgata attttgccaa ccacgatttt cgcatcagtt
    41041 gggttttcca gcaggtattc cgccagcagt tcgttcatct gctgttcaac cgccgatttc
    41101 acctcagaag aaaccagttt gtctttggtc tgggaggaga atttcgggtc cggcactttt
    41161 acggaaacga ccgcaatcag gccttcacgc gcatcgtcac cggtggcgct gactttggct
    41221 tttttgctgt agccttcttt gtccatgtag gcattcaggg tacgggtcat cgccgcacgg
    41281 aagcctgcca ggtgagtacc gccgtcacgc tgcggaatgt tgttggtaaa gcagtagatg
    41341 ttttcctgga agccatcgtt ccactgcagc gccacttcaa cgccaatacc gtctttttcg
    41401 gtggagaagt agaagatatt tgggtggatc ggcgttttgt tcttgttcag atattcaacg
    41461 aacgctttga tgccgccttc atagtggaag tggtcttctt tgccgtcacg cttgtcgcgc
    41521 agacgaatgg aaacgccgga gttgaggaac gacaactcac gcagacgttt cgccagaatt
    41581 tcatattcga actcggtcac attggtgaag gtttcgaggc tgggccagaa acgcaccatg
    41641 gtgccggttt tttcagtctc gccggtaacc gccagcggag cctgcggtac accgtgttcg
    41701 tagatctgac ggtgaatttt accctcgcgc tgaataacca gctccagttt ttgcgacagg
    41761 gcgtttacta ccgaaacacc aacgccgtgc agaccgccgg acactttata ggagttatcg
    41821 tcaaatttac cgcctgcgtg cagaacggtc atgatcactt ccgccgccga tacgccctct
    41881 tccgggtgaa taccggtcgg aatgccgcgc ccgtcatcct gtacagagac agagttatcg
    41941 gcgtgaatgg tgacgataat ttctttacag tgacccgcga gcgcttcgtc gatagcgtta
    42001 tctaccacct cgaataccat gtggtgcaga ccggtgccgt catccgtgtc gccgatatac
    42061 atacccgggc gcttacgcac cgcatccagc cctttcagga ctttgatact ggaggagtca
    42121 taagaattcg acatcaacgt ttctcgctca tttatacttg ggttaatccg ttattttacc
    42181 cttttccacg gtaaacatct tcgaattttc gtccgacatg tctataacgt gttcagcact
    42241 gatcgcgctg acaaagacct gtgattgcgt cgcttttaag cggctggcaa gcaacccgcg
    42301 acgctcatca tcaagctcag aggcaaaatc atctatcagg tagagacacc gccgcccgct
    42361 ttcacgagtg aggaactctc cttgcgccag acgtaaggcg cacatcaaca gcttaagctg
    42421 cccacgcgat aaggtatctt ccaccggcgc accgtcggcg cgaatgcgta agtccgcttt
    42481 atgcgggcca tgcgcggtgt aggttagctg gcgatcgcgt tcaaaattac gttccagcac
    42541 ctcagcgtac tctgtctctt tctcccagcc gcgctggaaa gagaaagtca gagaaaactc
    42601 agggagaaat tgcttacagg tatcatccat atcggccgcg ataccggcgc tatactccgc
    42661 gcgccaggtg ctgatttgct ccgccagcgg gatcagttct ttatcccacg ggcgtagctg
    42721 ttcgtaacga gtcacctggc gcagcgccgc attgcgctgc ttgagcaatc gcttgagatt
    42781 gctccaggcg gtgaaaaatc caggttcgtt gtgaaagcat ccccagtcga ggaatgctct
    42841 tctgtatttg gggccgccgt tgagtaaagt aaacccttct ggcgttatca actgcatcgg
    42901 catcaggtgc gccagttccg cgactttatg cccgtccgta ccgtcaatac ggactttgct
    42961 gtcgccctgt ttgtctttgg ttaagccaat cgccgtctcg cgctcttcgc cctgtaatcg
    43021 cccgtggaga acaaatgcct cctgctcatg gcgaatgacg cgaccaatct gcaaactgcg
    43081 aaacgcccga ccatggccga gcgtatagat ggcttccagc acgctggttt tgccactgcc
    43141 gttggcacct accagaaagt taaagccggg agataaagcg agatccgcgg tttcaatatt
    43201 gcggaaatcg cggatcaaca agcgggtgag ggacattaca gtctcattgg catgacaaca
    43261 taggcagccg actgtgatgc ggcatcttca atctgcacgc tggaaaccga atcggtcagc
    43321 atcatgcgga cgttttcgca tttcagcgcg ttcagaacat ccagcacata gctgacgttg
    43381 aagccgattt ccatctccgc accgctatag gtaacgtcga ggatctcttc cgcttcttcc
    43441 tgttccgggt tgttggcagt aattttcagc tggttttcgc tgacatacag gcgcacaccg
    43501 cggaatttct cgttagagag aatcgccgcg cgagcaaacg cctgcttgag cagatcgcag
    43561 cctgcttcca gatgtttgtc cgggttcttc ggcagaacgc gacggtaatc cgggaagcga
    43621 ccatccacca gtttcgaggt gaaaataaag tcgccaacgt gggcacgaat gttattgctg
    43681 ccaatctgca cacgcagcag attgtcgccg ccgtcgagca tacgcatcag ttcaatcacg
    43741 cctttacgcg gtacgatcac cgaatggctt ggcaaagatt gaccaattgg cattgaacag
    43801 accgccagac ggtggccgtc ggttgccacg gtgcgcagtt cttcaccttc ggtttcaaac
    43861 agcataccgt ttaagtaata gcgaacatcc tgatgcgcca tcgaaaactg ggttgcttca
    43921 atcagacgct tcatcgtcgc ctgcggcagg gtaaattcga cttcgctctg ccagtcatcg
    43981 aggttcggga aatccgccgc tggcagggta gacagcgaaa aacggctacg cccggagcgc
    44041 accagcatcc gttcaccttc cagctgcacg gcaatttccg cgccttcagg cagaccacgg
    44101 cagatatcaa agaatttgcg cgccggaacg gtcgtcgctc ctggctcgtg tggctgaacc
    44161 agcgcaacgc gtgccaccat ttccatctcg agatcagtac cggtcagcga caacgtaccg
    44221 tcagcaacct gtaacagcag attaccgaga atcggtagcg taggacgacc acctaacgga
    44281 ccgctcacct gttgtagcgg ttttaataaa tgctcacgtt ctacggtaaa tttcataggt
    44341 ttacgatgac aatgttctga ttaaatttga aaaatcttct ttgatatcgt ggctctcttc
    44401 acgcaactgc tcgatcttac ggcaggcatg aagcaccgtc gtgtggtcac gcccaccaaa
    44461 cgcatcgcca atctctggca gactgtggtt agtcagctct ttcgctagcg ccatcgccat
    44521 ctggcgcgga cgcgccaccg agcgggatcg acgcttggaa aggagatccg cgactttgat
    44581 cttgtagtac tccgccaccg tcttctgaat attgtcgatg gtgaccagtt tttcctgcaa
    44641 tgccagcaag tcgcgcagcg cctcacgcac gaagtcgatg gtgatcgccc gtccggtaaa
    44701 gttggcgtta gcaataacgc ggttcagcgc cccttccagc tcacgtacgt tagatcgtag
    44761 acgcttggcg ataaagaacg ccacttcacc cggcaaacga atgtcatttt cgtcggcctt
    44821 tttcatcagg atcgccacac gggtttccag ctctggcggt tcgatcgcca cagtcagtcc
    44881 ccaaccgaag cgggatttca aacgatcctc aacgccgttg atctctttcg gatagcgatc
    44941 cgaggtgaga atgatctgtt gattaccttc cagcagggcg ttgaaggtgt ggaaaaactc
    45001 ttcctgagat cgttctttat tagcaaaaaa ctgaatatcg tcgatcagca gtgcatctac
    45061 ggaacggtag tagcgtttaa actcttcgat cgcgttgttt tgcagggctt taaccatgtc
    45121 ctgaacaaag cgctcggagt gcatataaac cactttggca ttcggcttgc gcgccataat
    45181 gccgttaccc accgcatgca gcaggtgagt tttacccaga cccgtgccgc cataaaggaa
    45241 caacgggtta taggcaccgc cagggttatc cgccacctgg cgagccgccg cgcgcgccag
    45301 ttggttagat ttaccttcaa cgaagttatc aaacgtgtgt ttgacgttta cgttagaacg
    45361 ataggtcggt tctgccgggg ccgggacgtt atcccaacct gagcgcgtag aaggcgcagc
    45421 acgttgcggc tgcgtttgcg ccacctgtgc aggggccgcg acgttgctcg tcactgccgc
    45481 ttgtggcgtt tgcgtcaccg gtttggtgcc gacttcaaaa cgcagctgtg gggcatccgc
    45541 tccgcaaaaa ctggttagca gtccattgat attattaagg tacttgtccc gtacccaatc
    45601 gaggacaaaa cggtttggcg cgtacaggga cagcgtgtta tcgctcagtt ccgcctgcaa
    45661 tgggcgtatc cacatactga attctgtggc tggtaactca tcctgcaatc gggcaagaca
    45721 ctgctgccaa agcgaaagtg acacggcgga ctccactcga acaaaagtcg ataatgacta
    45781 aggctgaaac attcatgatt gttgacgtac gtcgaaaaga ccctgcttga gggtgacgca
    45841 cgaaccgctg tctgcggtta tatgcccgat caagatcctg caaaacgatc gggaccgcgg
    45901 atcatagcct aaactgcgca agagatcttc tgtttctcac agattttccc tatttatcca
    45961 caggactttc cagaactcgc taagtgtaaa cgatcctgcc gcgaggcggg cacgatttac
    46021 gccgcatatt ggaaaattta atgaccatag acaaaaattg gcttaatcga tctaataaag
    46081 atccaggacg atccttgcgc tttacccatc agcccgtata atcctccacc cggcgcgcca
    46141 tgctggtttc cactggtgtg aggtcgtaca ttttccctgc gaaaaggtgc ggaaaagcgc
    46201 ggtaaataag gaaagagaat tgactccggt gtgtacaatt attacaatcc ggcctcttta
    46261 atcacccatg gcttcggtgt ccatcgtttc atttttcggc ggatatccaa taaagccatt
    46321 gaatttattc aagtttaggt agaaatcgcc atgaaacgca cttttcaacc gtctgtactg
    46381 aagcgcaacc gttctcacgg cttccgtgct cgtatggcta ctaaaaatgg tcgtcaggtt
    46441 ctggcacgtc gtcgtgctaa aggccgcgct cgtctgaccg tttctaagta ataaagctaa
    46501 cccctgagtg gttaagctcg catttcccag ggagttacgc ttgttaactc ccagtcaatt
    46561 cacattcgtc ttccagcagc cacaacgggc tggcacgccg caaattacca ttctcggccg
    46621 cctgaattcg ctggggcatc cccgtatcgg tcttacagtc gccaagaaaa acgttcgacg
    46681 cgcccatgaa cgcaatcgga ttaaacgtct gacgcgtgaa agcttccgtc tgcgccaaca
    46741 tgaactcccg gctatggatt tcgtggtggt ggcgaaaaaa ggggttgccg acctcgataa
    46801 ccgtgctctc tcggaagcgt tggaaaaatt atggcgccgc cactgtcgcc tggctcgcgg
    46861 gtcctgatag ccctcattcg ggtctatcaa cgcctgatta gtccgctact cgggccgcat
    46921 tgtcgtttca ctccaacctg ttcaagctac ggaattgagg cattgcgcag gtttggagtg
    46981 ataaaaggca gttggttgac ggtgaaacgc gtattaaaat gccacccttt acaccctggt
    47041 ggtgacgatc ccgtcccgcc cggaccattt gataccagag aacactaacg atggattcgc
    47101 aacgcaatct tttagtcatc gctttgctgt tcgtgtcttt catgatctgg caagcctggg
    47161 agcaggataa aaacccgcaa cctcaggccc aacagaccac gcagacaacg accaccgcag
    47221 cgggtagcgc cgccgaccag ggcgtaccgg ccagtggcca ggggaaactg atctcggtta
    47281 agaccgacgt gcttgatctg accatcaaca cccgtggtgg tgatgttgag caagctctgc
    47341 tgcctgctta cccgaaagag ctgaactcta cccagccgtt ccagctgttg gaaacttcac
    47401 cgcagtttat ttatcaggca cagagcggtc tgaccggtcg tgatggcccg gataacccgg
    47461 ctaacggccc gcgtccgctg tataacgttg aaaaagacgc ttatgtgctg gctgaaggtc
    47521 aaaacgaact gcaggtgccg atgacgtata ccgacgcggc aggcaacacg tttaccaaaa
    47581 cgtttgtcct gaaacgtggt gattacgctg tcaacgtcaa ctacaacgtg cagaacgctg
    47641 gcgagaaacc gctggaaatc tcctcgtttg gtcagttgaa gcaatccatc actctgccac
    47701 cgcatctcga taccggaagc agcaacttcg cactgcacac cttccgtggc gcggcgtact
    47761 ccacgcctga cgagaagtat gagaaataca agttcgatac cattgccgat aacgaaaacc
    47821 tgaacatctc ttcgaaaggt ggttgggtgg cgatgctgca acagtatttc gcgacggcgt
    47881 ggatcccgca taacgacggt accaacaact tctataccgc taatctgggt aacggcatcg
    47941 tcgctatcgg ctataaatct cagccggtac tggttcagcc tggtcagact ggcgcgatga
    48001 acagcaccct gtgggttggc ccggaaatcc aggacaaaat ggcagctgtt gctccgcacc
    48061 tggatctgac cgttgattac ggttggttgt ggttcatctc tcagccgctg ttcaaactgc
    48121 tgaaatggat ccatagcttt gtgggtaact ggggcttctc cattatcatc atcaccttta
    48181 tcgttcgtgg catcatgtac ccgctgacca aagcgcagta cacctccatg gcgaagatgc
    48241 gtatgctgca gccgaagatt caggcaatgc gtgagcgtct gggcgatgac aaacagcgta
    48301 tcagtcagga aatgatggcg ctgtacaaag ctgagaaggt taacccgctg ggcggctgct
    48361 tcccgctgct gatccagatg ccaatcttcc tggcgttgta ctacatgctg atgggttccg
    48421 ttgaactgcg tcaggcaccg tttgcactgt ggatccacga cctgtcggca caggacccgt
    48481 actacatcct gccgatcctg atgggcgtaa cgatgttctt cattcagaag atgtcgccga
    48541 ccactgtgac cgacccgatg cagcagaaga tcatgacctt tatgccggtc atcttcaccg
    48601 tgttcttcct gtggttcccg tcaggtctgg tactgtacta tatcgtcagc aacctggtaa
    48661 ccattattca gcagcagctg atttaccgtg gtctggaaaa acgtggcctg catagccgcg
    48721 agaagaaaaa atcctgattc ggtgagtttt cgctaaaata agggcggtca gttgaccgcc
    48781 ttttttcttt tcgtagggcg gataagcacc gcgcatccgc cacacaaagc aacaggaaca
    48841 tcatgagcga taatgacact atcgtagccc aggccacacc tccgggacgt ggcggcgttg
    48901 gcatcctgcg catctccggc ttcaaagccc gtgaagttgc cgaaaccgtg ctgggtaaac
    48961 tgcctaagcc gcgctacgcc gattatcttc cgtttaaaga cgccgacggc agcgtgctcg
    49021 atcaggggat tgcgctatgg ttccctggcc cgaactcgtt caccggcgaa gatgtgctgg
    49081 aactgcaagg tcatggcggt ccggtgatcc tcgacctgct gttaaaacgc attctgacca
    49141 ttcccggcct gcggattgct cgccctggtg agttttccga acgcgcgttt cttaacgata
    49201 aacttgactt agcccaggcc gaggcgattg ccgatcttat cgacgccagt tcggaacagg
    49261 cggcccgttc ggcacttaac tcgctgcaag gcgcattctc cgcacgggtt aatcatctgg
    49321 tagaagccct cacccacttg cgcatttacg tcgaagcggc aattgatttc cctgatgaag
    49381 agatcgattt cctctccgac ggcaaaattg aagcccagct taatgacgtg atggccgatc
    49441 tcgatgcagt gcgtgctgaa gcacgtcagg gtagtttgtt gcgcgaaggg atgaaagtag
    49501 tgattgccgg acgtcctaat gccggtaaat cgagcctgtt aaacgcgctg gcgggccgtg
    49561 aagcggcaat cgtaaccgat atcgccggaa ccacgcgtga cgtgctgcgt gagcatatcc
    49621 acattgacgg aatgccgctg catatcatcg acactgccgg gctacgtgag gccagtgacg
    49681 aagtggaacg tattggtatc gagcgcgcgt ggcaggaaat tgaacaggcc gaccgcgtgc
    49741 tgtttatggt cgatggcacc acgacagacg ccgttgatcc ggcagagatc tggccggaat
    49801 ttatcgcccg tctgcccgcg aaactgccga tcaccgtggt acgcaataaa gccgatatca
    49861 ccggcgaaac gctgggaatg agtgaagtga acggtcacgc gttaattcgt ctctcggcaa
    49921 ggactggtga aggcgtggac gtgctgcgta accatctcaa acagagcatg ggctttgaca
    49981 ccaatatgga aggcggcttc ctggcgcgcc gtcgccactt acaggcgctg gaacaggcag
    50041 cggagcattt gcagcaaggt aaagcgcaac tgttgggagc ctgggcaggt gaactgctgg
    50101 cggaagagtt gcgcctggca cagcagaact taagcgaaat caccggggaa tttacttcag
    50161 acgacctgct ggggcggatt ttctccagct tctgtattgg taagtaaccg cgcttacgaa
    50221 gccgtattct gactgtcaga tgcggcttcg cttcattgtt agcactcctg ttatgcctca
    50281 accctttttt aaacattaaa attcttacgt aatttataat ctttaaaaaa agcatttaat
    50341 actgctcccc gaacgattgt gattcgattc acatttaaac aatttcagaa tagacaaaaa
    50401 ttctgagtgt aataatgtag cggcgtgtct ttcgaggata agtgcattat gaatatctta
    50461 catgtatgtg tgacctcaaa atggttcaat atcgacaata agattgtcga tcaccgccct
    50521 taatttaccc ttctgtagcc atcaccagag ccaaaccgat tagattcaat gtgatcgatt
    50581 agtttgctat atcttaattt tgccttttgc aaaggccatc tctcgtttat ttacttattt
    50641 tagtaaatga tggtgcttgt atatatacat ggtgaattaa tggatattgc agatgtaata
    50701 ttcacaggga tcactgtaat taaaataaat gaaggattat gtaatggaaa actttaaaca
    50761 tttccctgaa ccgttccgca ttcgtgttat tgagccagta aaacgtacta ctcgcgctta
    50821 tcgtgaagaa gcaattatta aatccggtat gaacccgttc ctgctggata gcgaagatgt
    50881 gtttatcgat ttactgaccg acagcggcac cggggcggta acccaaagta tgcaggcagc
    50941 gatgatgcgc ggcgacgaag cctacagcgg cagccgcagc tactatgcgt tagccgagtc
    51001 agtgaaaaat atctttggtt atcaatatac tattccgact caccagggcc gtggcgcaga
    51061 gcaaatctat attccggtac tgattaaaaa acgcgagcag gaaaaaggcc tggatcgcag
    51121 caaaatggtg gcattctcta actatttctt tgataccacg cagggccata gccagattaa
    51181 cggctgtacc gtgcgtaacg tctatatcaa agaagccttc gatacgggcg tgcgttacga
    51241 ctttaaaggc aactttgacc tcgaaggatt agaacgcggt attgaagaag ttggcccgaa
    51301 taacgtgccg tatatcgttg caaccatcac cagtaactct gcaggtggtc agccggtttc
    51361 actggcaaac ttaaaagcga tgtacagcat cgcgaagaaa tacgatattc cggtggtaat
    51421 ggactccgca cgctttgctg aaaacgccta tttcatcaag cagcgtgaag cagaatacaa
    51481 agactggacc atcgagcaga tcacccgcga aacctacaaa tatgccgata tgctggcgat
    51541 gtccgccaag aaagatgcga tggtgccgat gggcggcttg ctgtgcatga aagacgacag
    51601 cttctttgat gtgtacaccg agtgcagaac cctttgcgtg gtgcaggaag gcttcccgac
    51661 atatggcggc ctggaaggcg gcgcgatgga gcgtctggcg gtaggtctgt atgacggcat
    51721 gaatctcgac tggctggctt atcgtatcgc gcaggtacag tatctggtcg atggtctgga
    51781 agagattggc gttgtctgcc agcaggcggg cggtcacgcg gcattcgttg atgccggtaa
    51841 actgctgccg catatcccgg cagaccagtt cccggcacag gcgctggcgt gcgagctgta
    51901 taaagtcgcc ggtatccgtg cggtagaaat tggctctttc ctgttaggcc gcgatccgaa
    51961 aaccggtaaa caactgccat gcccggctga actgctgcgt ttaaccattc cgcgcgcaac
    52021 atatactcaa acacatatgg acttcattat tgaagccttt aaacatgtaa aagagaacgc
    52081 ggcgaatatt aaaggattaa cctttaccta cgaaccaaaa gtattgcgtc acttcaccgc
    52141 aaaactgaaa gaagtttaat taatactaca gagtggctat aaggatgtta gccactctct
    52201 taccctacat cctcaataac aaaaatagcc ttcctctaaa ggtggcatca tgactgatca
    52261 agctgaaaaa aagcactctg cattttgggg tgttatggtt atagcaggta cagtaattgg
    52321 tggaggtatg tttgctttac ctgttgatct tgccggtgcc tggtttttct ggagtgcctt
    52381 tatccttatc attgcctggt tttcaatgct tcattccggg ttattgttat tagaagcaaa
    52441 tttaaattat cctgtcggct ccagttttaa caccatcacc aaagatttaa taggtaacac
    52501 ctggaacatt atcagcggta ttaccgttgc cttcgttctc tatatcctca cttatgccta
    52561 tatctctgct aatggtgcga tcattagtga aacgatatca atgaatttgg gctatcacgc
    52621 taatccacgt attgtcggga tctgcacagc cattttcgtt gccagcgtat tgtggataag
    52681 ttcgttagcc gccagtcgca ttacctcatt gttcctcggg ctgaagatta tctcctttgt
    52741 gatcgtgttt ggttctttct tcttccaggt cgattactcc atcctgcgcg atgccaccag
    52801 caccactgcg ggaacgtctt acttcccgta tatctttatg gctttgccgg tgtgtctggc
    52861 gtcatttggt ttccacggca atattcccag cctgattatt tgctatggca aacgcaaaga
    52921 taagttaatc aaaagcgtgg tatttggttc gctgctggcg ctggtgattt atctcttctg
    52981 gctctattgc acgatgggga atattccgcg cgaaagcttt aaggcgataa tctcctcagg
    53041 cggcaacgtt gattcgctgg tgaaatcgtt cctcggcacc aaacagcacg gcattatcga
    53101 gttttgcctg ctggtgttct ctaacttagc tgttgccagt tcgttctttg gtgtcacgct
    53161 ggggttgttc gattatctgg cggacctgtt taagattgat aactcccacg gcgggcgttt
    53221 caaaaccgtg ctgttaacct tcctgccacc tgcgttgttg tatctgatct tcccgaacgg
    53281 ctttatttac gggatcggcg gtgccaggct gtgcgccacc atctgggcgg tcattattcc
    53341 cgcagtgctt gcaatcaaag ctcgcaagaa gtttcccaat cagatgttca cggtctgggg
    53401 cggcaatctt attccggcga ttgtcattct ctttggtata accgtgattt tgtgctggtt
    53461 cggcaacgtc tttaacgtgt tacctaaatt tggctaaatc cttcaagaag ccagccattc
    53521 gctggcttct tgcctctcag gaaatcactt atgtccaaat ggcaactcgc cttatcctcc
    53581 ttcaccacgt atgctttgcg tcaccttact atcaggacgc tttagcccat gtcccgcttt
    53641 ttgatttgta gttttgccct ggttttactt tatcccgccg ggattgatat gtacctcgtt
    53701 ggtttaccgc gcatcgccgc cgatctcaat gccagcgaag cgcagttgca tattgcgttc
    53761 tccgtatatc tggcggggat ggcagctgcg atgctatttg ccggtaaagt ggccgatcgt
    53821 tcagggagaa agccggtcgc catacccggc gcggcgctat ttattattgc ctcggtgttc
    53881 tgttcactgg ctgaaaccag cacgttattt cttgcaggcc gatttctaca ggggttgggc
    53941 gcaggctgtt gttacgtggt ggcgttcgcc attttgcgcg acacgctgga tgatcgacgt
    54001 cgggctaaag tgctgtcatt actcaacggt attacctgca tcattccggt gttagcgcca
    54061 gtgctcggac atctgattat gcttaaattc ccgtggcaga gtctgttctg ggcgatggca
    54121 atgatgggca tcgcggtact gatgttgtct ttgtttattt taaaagaaac gcgcccagcg
    54181 tcccccgctg cttcggacaa accacgagaa aatagcgagt cgctgcttaa tcgttttttc
    54241 ctcagccgtg ttgttatcac caccctcagc gtttcggtga tcctcacttt cgtcaataca
    54301 tcgccggtat tgctgatgga aatcatgggg tttgagcgcg gagaatacgc caccattatg
    54361 gcgctgaccg ctggcgtcag catgaccttt tcattctcga cgccatttgc gctgggaatt
    54421 tttaagccac gtacgttgat gatcacctcg caggtgttat tccttgcggc ggggatcacc
    54481 cttgccgttt caccttccca tgcggtttct ctgtttggta tcacgctgat ttgcgccggt
    54541 ttctcggtag gttttggcgt agcgatgagt caggcgttag gaccattttc attacgcgcg
    54601 ggcgtagcca gctcgacctt aggtattgcg caggtttgcg gttcgtcact gtggatttgg
    54661 ctggccgcgg tggttggtat cggcgcatgg aatatgctga tcgggattct gattgcctgt
    54721 agcattgtga gcctgttgct gattatgttc gtcgcgcctg gacgccccgt tgccgctcat
    54781 gaagaaatcc atcaccacgc ttgatctcaa tctgctgctc tgtctgcaac tgctgatgca
    54841 ggagcgcagc gtgaccaaag cggcgaagcg gatgaacgtg accccttcag cggtgagtaa
    54901 gtcgctggca aagttaagag cgtggtttga cgacccgctc tttgtgaact caccgctggg
    54961 gctgtcgccc acacagctaa tggtcagcat ggagcaaaat ctggcggaat ggatgcaaat
    55021 gagcaatctg ctgctggata aaccgcacca ccagacaccg cgcggcctga agtttgagtt
    55081 ggcggcggaa tcgccgctga tgatgatcat gcttaatgcg ctgtcgaaac ggatctacca
    55141 acgttacccg caggcgacca tcaaactacg taactgggat tacgattcct tagatgccat
    55201 tactcgtggt gaagtggata ttggtttttc cggtcgcgaa agccatcctc gctcgcggga
    55261 gctgttaagc tcgctaccgt tagccattga ttatgaagtg ctgtttagtg atgtaccctg
    55321 cgtctggtta cgcaaagatc atccggcact gcatgaaacg tggaatctgg acaccttttt
    55381 acgttatccg catatcagca tttgctggga acagagcgac acctgg
//
LOCUS       DAETPZ010000019        55342 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.019, whole genome shotgun sequence.
ACCESSION   DAETPZ010000019 DAETPZ010000000
VERSION     DAETPZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.019"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..355
                     /locus_tag="K0W59_001264"
     CDS             <1..355
                     /locus_tag="K0W59_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HBI3022379.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(393..1292)
                     /gene="nhaR"
                     /locus_tag="K0W59_001265"
     CDS             complement(393..1292)
                     /gene="nhaR"
                     /locus_tag="K0W59_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HBI3022380.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1358..2524)
                     /gene="nhaA"
                     /locus_tag="K0W59_001266"
     CDS             complement(1358..2524)
                     /gene="nhaA"
                     /locus_tag="K0W59_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HBI3022381.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLPFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFSNAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3110..3262
                     /gene="mokC"
                     /locus_tag="K0W59_001267"
     CDS             3110..3262
                     /gene="mokC"
                     /locus_tag="K0W59_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HBI3022382.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3366..4496)
                     /gene="dnaJ"
                     /locus_tag="K0W59_001268"
     CDS             complement(3366..4496)
                     /gene="dnaJ"
                     /locus_tag="K0W59_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBI3022383.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4585..6501)
                     /gene="dnaK"
                     /locus_tag="K0W59_001269"
     CDS             complement(4585..6501)
                     /gene="dnaK"
                     /locus_tag="K0W59_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBI3022384.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRATD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSSRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6878..7282
                     /locus_tag="K0W59_001270"
     CDS             6878..7282
                     /locus_tag="K0W59_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HBI3022385.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7308..8021
                     /gene="msyB"
                     /locus_tag="K0W59_001271"
     CDS             7308..8021
                     /gene="msyB"
                     /locus_tag="K0W59_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HBI3022386.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8170..8736
                     /gene="satP"
                     /locus_tag="K0W59_001272"
     CDS             8170..8736
                     /gene="satP"
                     /locus_tag="K0W59_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HBI3022387.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8771..9358)
                     /gene="mog"
                     /locus_tag="K0W59_001273"
     CDS             complement(8771..9358)
                     /gene="mog"
                     /locus_tag="K0W59_001273"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HBI3022388.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9473..10426)
                     /gene="tal"
                     /locus_tag="K0W59_001274"
     CDS             complement(9473..10426)
                     /gene="tal"
                     /locus_tag="K0W59_001274"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBI3022389.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10705..12177
                     /locus_tag="K0W59_001275"
                     /pseudo
     CDS             10705..12177
                     /locus_tag="K0W59_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12247..13023
                     /gene="yaaA"
                     /locus_tag="K0W59_001276"
     CDS             12247..13023
                     /gene="yaaA"
                     /locus_tag="K0W59_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBI3022390.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13176..13472)
                     /locus_tag="K0W59_001277"
     CDS             complement(13176..13472)
                     /locus_tag="K0W59_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBI3022391.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13686..14972)
                     /gene="thrC"
                     /locus_tag="K0W59_001278"
     CDS             complement(13686..14972)
                     /gene="thrC"
                     /locus_tag="K0W59_001278"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBI3022392.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14973..15905)
                     /gene="thrB"
                     /locus_tag="K0W59_001279"
     CDS             complement(14973..15905)
                     /gene="thrB"
                     /locus_tag="K0W59_001279"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBI3022393.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15907..18369)
                     /gene="thrA"
                     /locus_tag="K0W59_001280"
     CDS             complement(15907..18369)
                     /gene="thrA"
                     /locus_tag="K0W59_001280"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HBI3022394.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18395..18514)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18450..18515)
                     /gene="thrL"
                     /locus_tag="K0W59_001281"
     CDS             complement(18450..18515)
                     /gene="thrL"
                     /locus_tag="K0W59_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HBI3022395.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18729..19415)
                     /locus_tag="K0W59_001282"
     CDS             complement(18729..19415)
                     /locus_tag="K0W59_001282"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBI3022396.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSSDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19815..19955)
                     /locus_tag="K0W59_001283"
     CDS             complement(19815..19955)
                     /locus_tag="K0W59_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022397.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20051..20767
                     /gene="arcA"
                     /locus_tag="K0W59_001284"
     CDS             20051..20767
                     /gene="arcA"
                     /locus_tag="K0W59_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HBI3022398.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20826..22178)
                     /gene="creD"
                     /locus_tag="K0W59_001285"
     CDS             complement(20826..22178)
                     /gene="creD"
                     /locus_tag="K0W59_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HBI3022399.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22236..23660)
                     /gene="creC"
                     /locus_tag="K0W59_001286"
     CDS             complement(22236..23660)
                     /gene="creC"
                     /locus_tag="K0W59_001286"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HBI3022400.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNTIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRNFT"
     gene            complement(23660..24349)
                     /gene="creB"
                     /locus_tag="K0W59_001287"
     CDS             complement(23660..24349)
                     /gene="creB"
                     /locus_tag="K0W59_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HBI3022401.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGVDDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24362..24835)
                     /gene="creA"
                     /locus_tag="K0W59_001288"
     CDS             complement(24362..24835)
                     /gene="creA"
                     /locus_tag="K0W59_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HBI3022402.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25046..25915
                     /gene="robA"
                     /locus_tag="K0W59_001289"
     CDS             25046..25915
                     /gene="robA"
                     /locus_tag="K0W59_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HBI3022403.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGKFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25912..26559)
                     /gene="gpmB"
                     /locus_tag="K0W59_001290"
     CDS             complement(25912..26559)
                     /gene="gpmB"
                     /locus_tag="K0W59_001290"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HBI3022404.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26611..27132
                     /gene="yjjX"
                     /locus_tag="K0W59_001291"
     CDS             26611..27132
                     /gene="yjjX"
                     /locus_tag="K0W59_001291"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HBI3022405.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(27217..27543)
                     /gene="trpR"
                     /locus_tag="K0W59_001292"
     CDS             complement(27217..27543)
                     /gene="trpR"
                     /locus_tag="K0W59_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HBI3022406.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27633..29570)
                     /gene="sltY"
                     /locus_tag="K0W59_001293"
     CDS             complement(27633..29570)
                     /gene="sltY"
                     /locus_tag="K0W59_001293"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HBI3022407.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29781..31448
                     /gene="ettA"
                     /locus_tag="K0W59_001294"
     CDS             29781..31448
                     /gene="ettA"
                     /locus_tag="K0W59_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HBI3022408.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLDQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGSQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31555..31722)
                     /locus_tag="K0W59_001295"
     CDS             complement(31555..31722)
                     /locus_tag="K0W59_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022409.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31756..32988)
                     /gene="nadR"
                     /locus_tag="K0W59_001296"
     CDS             complement(31756..32988)
                     /gene="nadR"
                     /locus_tag="K0W59_001296"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HBI3022410.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(33009..34391)
                     /gene="radA"
                     /locus_tag="K0W59_001297"
     CDS             complement(33009..34391)
                     /gene="radA"
                     /locus_tag="K0W59_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBI3022411.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34440..35408)
                     /gene="serB"
                     /locus_tag="K0W59_001298"
     CDS             complement(34440..35408)
                     /gene="serB"
                     /locus_tag="K0W59_001298"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HBI3022412.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAARSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVLANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35514..36158
                     /locus_tag="K0W59_001299"
     CDS             35514..36158
                     /locus_tag="K0W59_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HBI3022413.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTQLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36186..37202
                     /gene="lplA"
                     /locus_tag="K0W59_001300"
                     /pseudo
     CDS             36186..37202
                     /gene="lplA"
                     /locus_tag="K0W59_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37234..37497
                     /locus_tag="K0W59_001301"
     CDS             37234..37497
                     /locus_tag="K0W59_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3022414.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37658..38377)
                     /gene="deoD"
                     /locus_tag="K0W59_001302"
     CDS             complement(37658..38377)
                     /gene="deoD"
                     /locus_tag="K0W59_001302"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBI3022415.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38434..39657)
                     /gene="deoB"
                     /locus_tag="K0W59_001303"
     CDS             complement(38434..39657)
                     /gene="deoB"
                     /locus_tag="K0W59_001303"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBI3022416.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLIRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39709..41031)
                     /gene="deoA"
                     /locus_tag="K0W59_001304"
     CDS             complement(39709..41031)
                     /gene="deoA"
                     /locus_tag="K0W59_001304"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HBI3022417.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MVLCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDH
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41158..41937)
                     /gene="deoC"
                     /locus_tag="K0W59_001305"
     CDS             complement(41158..41937)
                     /gene="deoC"
                     /locus_tag="K0W59_001305"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBI3022418.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRYMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42195..43736
                     /locus_tag="K0W59_001306"
     CDS             42195..43736
                     /locus_tag="K0W59_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HBI3022419.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSL"
     gene            43708..44571
                     /locus_tag="K0W59_001307"
     CDS             43708..44571
                     /locus_tag="K0W59_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HBI3022420.1"
                     /translation="MNSRCALVCKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEVTTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44785..45567)
                     /locus_tag="K0W59_001308"
     CDS             complement(44785..45567)
                     /locus_tag="K0W59_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBI3022421.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45564..46637)
                     /locus_tag="K0W59_001309"
     CDS             complement(45564..46637)
                     /locus_tag="K0W59_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HBI3022422.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46759..46920)
                     /locus_tag="K0W59_001310"
     CDS             complement(46759..46920)
                     /locus_tag="K0W59_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HBI3022423.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(47047..47652)
                     /gene="osmY"
                     /locus_tag="K0W59_001311"
     CDS             complement(47047..47652)
                     /gene="osmY"
                     /locus_tag="K0W59_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HBI3022424.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKDYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(48045..49634)
                     /gene="prfC"
                     /locus_tag="K0W59_001312"
     CDS             complement(48045..49634)
                     /gene="prfC"
                     /locus_tag="K0W59_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBI3022425.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49725..50402)
                     /gene="yjjG"
                     /locus_tag="K0W59_001313"
     CDS             complement(49725..50402)
                     /gene="yjjG"
                     /locus_tag="K0W59_001313"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HBI3022426.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50417..50863)
                     /gene="rimI"
                     /locus_tag="K0W59_001314"
     CDS             complement(50417..50863)
                     /gene="rimI"
                     /locus_tag="K0W59_001314"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBI3022427.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50832..51245)
                     /gene="holD"
                     /locus_tag="K0W59_001315"
     CDS             complement(50832..51245)
                     /gene="holD"
                     /locus_tag="K0W59_001315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HBI3022428.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRVNPTARAALWQQICTYEHDFFPRND"
     gene            51348..52379
                     /gene="rsmC"
                     /locus_tag="K0W59_001316"
     CDS             51348..52379
                     /gene="rsmC"
                     /locus_tag="K0W59_001316"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HBI3022429.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            52578..52664
                     /locus_tag="K0W59_001317"
     tRNA            52578..52664
                     /locus_tag="K0W59_001317"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52612..52614,aa:Leu,seq:cag)
     gene            52693..52779
                     /locus_tag="K0W59_001318"
     tRNA            52693..52779
                     /locus_tag="K0W59_001318"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52727..52729,aa:Leu,seq:cag)
     gene            52814..52900
                     /locus_tag="K0W59_001319"
     tRNA            52814..52900
                     /locus_tag="K0W59_001319"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52848..52850,aa:Leu,seq:cag)
     gene            complement(52939..53175)
                     /locus_tag="K0W59_001320"
     CDS             complement(52939..53175)
                     /locus_tag="K0W59_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HBI3022430.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            53316..54104
                     /gene="fhuF"
                     /locus_tag="K0W59_001321"
     CDS             53316..54104
                     /gene="fhuF"
                     /locus_tag="K0W59_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HBI3022431.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            complement(54142..54819)
                     /gene="bglJ"
                     /locus_tag="K0W59_001322"
     CDS             complement(54142..54819)
                     /gene="bglJ"
                     /locus_tag="K0W59_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HBI3022432.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(54777..>55342)
                     /locus_tag="K0W59_001323"
     CDS             complement(54777..>55342)
                     /locus_tag="K0W59_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBI3022433.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        13252 a        14920 c        13823 g        13347 t
ORIGIN      
        1 ttatcagttt catcatataa gatttgactg catccagggc gagcaaggtg aattagtttt
       61 tgaacccagt gacgttgaat attactttga accagtgacg atacaggagt caggaaccag
      121 catattaaaa aatgagttag aaaacagtga ggacggtgcg ggtcaaatag gctttcaact
      181 ctccaatgat ggtacgaatg aaatccagta tggtaaaagc aattactact cttttcagca
      241 tccccatgaa ggaagtaatc agattcctct atttattcgc ccgcgtacat atggaaacaa
      301 tgtctcaagc gggcaaataa tgagccgagt caaaatcgtg gtaatgtaca actaatctat
      361 ctcccccacc ttaactttgg gggagctgcc gattaacgca ccgctggact aaaaagcgca
      421 gaataatccg tattgcagat tcgctgtacc gccgggtgct gaatcatccg ctcagcaaaa
      481 atagcatggt actcttccat cacattctcg acgcgaccaa tttctacgac agttttatcg
      541 gcataaaaat catatgcata aagcgttggg gcaacgaaga ttgcattgtg catcgcacca
      601 aaagctttca tcaaagcggc atcatcaaac tcgccgagga tttctacgtt taatccctgg
      661 gagttaaacc agttaagcaa tttgcgccct aacattgaac gtcgcccagg aatcaaaagt
      721 cgccgttctt ccagacaagc cgggaacggt ttttctggtg gtggatttgt acaccagaaa
      781 ctcacgccac attcgccaat tctcacggag aacaggcctt cctgctgcgt agagtctatc
      841 ggacagtcag aaatgatcat atccagttta tgctgactta attgctccag cagcatttcg
      901 tgggtggatt cgaagcagcg aagatgaatg ggctcgcctt ctaccactgc ggcgttaagt
      961 acgctactga ccaggcgttt ggaaagtgca tcagccacgc caacgtcaaa caataaattg
     1021 gattctttgc gatagttcac aatatccagc atttcctggc ttaaggtgaa cattttatcg
     1081 gcatagcgat agaccagttc tcccagctcg ctgggttcga gaccacgtcc cttgcgttta
     1141 aataatttgc cttgcaggcg ctcttccagc gctcgaatct gtccggtaat ggtttgtgga
     1201 gttaaataaa gcgcctccgc tgcgccaacc acggaacctt ctttatagac atgccagaag
     1261 taatacaagt ggttgtaatt gatatgagac atgctcattt ctctccctga taacaatgaa
     1321 aagggagccg tttatggctc cccggtaaac cgtcctgtca aactgatgga cgcaaacgaa
     1381 cgcgtaacca gctgtatcca attaccgccg aagagataga accgaccagg ataccgagtt
     1441 tcgcccagtt aatcagttct ggatctacgc taccaaaggc caggctggca ataaagatag
     1501 acatagtaaa accgataccg cacaggatcc ccaccaccat aatttgctga taagtcgttc
     1561 cctcaggcag atgcgccagt ttcaaacgca gcgccaacca gcagaacaga ctaatcccca
     1621 gcggtttgcc aatcagcaaa ccagcgatga tccccaatgg cagaatggag gtcaagccat
     1681 ccagcgtgac gccttgcagt gaaacgccag cattagaaaa tgcaaacagc ggcaaaatca
     1741 gatacgccac ccacgggtgc aacacatgct ccagtcgctt cgctggagaa cgcccatgct
     1801 tctctttcaa aggaataaag aagccgacaa ttacccccgc cagagttgcg tgaacccccg
     1861 atttcaacac cgcagtccac aacaccacgc caacaagaat atagacgccc gtgcggcgta
     1921 caccacacag attcaatacc gcgagtaccg caattgctac agccgcgacg ccaagagagg
     1981 ccatcgataa gtcattagtg tagaacaatg cgatgataat gatggcccca agatcgtcga
     2041 taatagccag agccatcaaa aagatcttca gcgctaacgg aacacgactt cccaacagcg
     2101 ccagtacacc aagtgcaaaa gcaatgtcag tagccgccgg gatcgcccac ccttcgcggg
     2161 taatcggatc ggcatagtta aaaggcagat agagtaatgc cggcacaatc atcccaccaa
     2221 tagcggcgat aactggaaat gcggcctggc gtaagctggc tagcgatcct tgcatcagtt
     2281 cacgtttaac ttccagaccg actaacagga aaaataccgc catcagcgcg tcatttatcc
     2341 ataacagcat gtttttgttg atttcgagtg aaccaacccg gagctgaacc ggcgtctcca
     2401 gaaagtcgtg ataccatcca ctggttgcgc cgctgttggc cataatcatc gccaggatag
     2461 cggcaatgat aagtataatg cctcccgagg catcactgct aaagaatcga tgcagatgtt
     2521 tcacttttta tttctctttc aggtgaatag atcgttttta ctattttacc ccgcacgaat
     2581 catcgttaaa atcagattat agtgtaggaa taattcgttt tttacgagct attgaagaat
     2641 ttgcgccatc agcggtgaga tgcgtttttg acggctgtaa gcgctcacga gtgaccctaa
     2701 gcccagataa tcatagctta aggtgaggat tcctgaggga ttaattagga aaagtaataa
     2761 ttaatttgtt gtcagtcggc gatgcttgtt aggccaatat ttcaaaaata atccagcttg
     2821 tcgtcaataa ttgcgatgaa gagataaatt aaagttaaat aattaaatca ttaaaaaatt
     2881 acgccacaaa aatgcgaggc gtcttcagga ttagaatcga taaaacaaaa tgggttaaag
     2941 agagcgatat aatagcggcg ggtgcttgag gctgtctgtc tcaggcatta gctgaacggc
     3001 agatagagaa aagccccgag tgatatttta ccatcaaccc gaggcctcct atatgctgaa
     3061 cacatgtaga gtgcctctta ctgaccgtaa ggtcaaggag aagagagcaa tgaagcagca
     3121 taaggcgatg attgtcgccc tgatcgtcat ctgtatcacc gccgtagtgg cggcgctggt
     3181 gacgagaaaa gacctctgtg aggttcacat ccgaactggc cagacggagg ttgctgtttt
     3241 cacggcttac gaatccgagt aagagcaacg gcggggagtg atccccgcca ctttacaggt
     3301 gctcgcatat cttcaacgca ccctattttt acccaggcct gcccacgggc aggcttttgg
     3361 ggaggttagc gggtcaggtc gtcaaaaaac ttcttcacac catcaaagaa gctctttgag
     3421 cgcgggctgt tgtgctcgcc ggttgggcca ccgaagcttt cttgcagctc ttgcagcagc
     3481 tgtttctgct tttcgttcag gcctaccggt gtttcgacga caacgcggca cagcaaatca
     3541 ccctgtgcac caccgcggac agacttgacg cctttaccgc gcatacggaa tagcttaccg
     3601 gtctgggttt cgccaggcac tttcagtttg acgcgaccat caagggtcgg tacttcgatt
     3661 tcgccaccca gcgccgccat agcgaagttg atcgggactt cgcaatacag gttgttgcct
     3721 tcacgctcga aaatcgggtg ctgtttaacc tgaacctgaa cgtacagatc gcctgccggt
     3781 gcgccatgct cgcccgcttc accttcgccc gcaagacgga tgcggtctcc agtgtccacc
     3841 cctgccggga ttttaacgga cagcgttttg ctgcgctcaa cacgaccatg accatgacat
     3901 ttgttgcacg gatctttgat cagcgtaccg cggccctgac agtgtggaca ggtctgctgt
     3961 acagcgaaga atccctggcg catctgcacc tgaccagaac catgacaggt cggacaggtc
     4021 tgcggctgtg tacctggttt tgcaccgcta ccgtggcaaa cgtcacactc ttccagagtc
     4081 ggaatgcgga tttctttggt cacgccacgt acagcttctt cgagggtgag ctccatgtta
     4141 tagcgtaaat cagcaccgcg cgccgcacgt tgacgaccac gtccgccgcc aaaaatatcg
     4201 ccgaaaacgt caccaaaaat atcgctgaag tctgcgccgc cgccaaaacc accgccgccc
     4261 atgcctcctt gctcaaacgc agcatgacca tactgatcgt atgccgcacg tttttgcgag
     4321 tcggtcagaa cttcataagc ttccttgatc tctttaaatt tcgcctcggc ctctttgtca
     4381 ccctggttac ggtccgggtg gtatttcatg gccaggcgtt tgtaggcctt tctgatttca
     4441 cgctcttccg ctgttttgga aacgcctaaa atctcgtaat aatcttgctt agccatcttt
     4501 tttaaattgc ccctagatga atccacgggc ggagaggaaa ttccccttcg cccgtgtcag
     4561 tataattacc cgtttatagg gcgattattt tttgtctttg acttcttcaa attcagcgtc
     4621 gacaacatcg tcatctttcg cgttgtttgc agaagcatca gcaccggcag tctgctgctg
     4681 ggcatgttgc tgctgggcga tttccatcag tttctgggaa acctgtgcca gttcctgcat
     4741 tttcgcttcg atagcggctt tgtcttcacc tttcagagca gtttccagtg cagtcagcgc
     4801 agactcgata gcagttttgt cgtcagccgg cagtttgtcg cctgcttctt caacctgctt
     4861 acgggagctg tgcagcagat ggtcgccctg gttgcgagtc tgtaccagct cttcaaactt
     4921 acggtcagct tcggcgttag cttctgcgtc gcgtaccatt ttctggattt catcttcgtt
     4981 cagaccagaa gaagccttga tggtgatctt ctgctcttta ccgctgtttt tatctttcgc
     5041 ggaaacgtgc aggataccgt cagcatcgat atcgaaggta acttcgatct gcggcatgcc
     5101 gcgcggtgcc gggttgatac catctaggtt gaactgaccc agagatttgt tatcagtcgc
     5161 acgtttacgt tcaccctgca gcacatggat ggttaccgca gactggttgt cttcagcggt
     5221 agagaacacc tggctgtgct tggtcgggat agtggtgttt ttcgcgatca gcgtcgtcat
     5281 cacaccgccc atggtttcga tacccagaga cagcggggta acgtccagca gcagtacgtc
     5341 ttttacgtca ccagtcagaa caccaccctg aacagcagca ccgattgcta cagcttcgtc
     5401 cgggttaacg tctttacgcg gctctttacc aaagaactca gcaactttct tctgaaccat
     5461 tggcatacga gtctgaccac caacgaggat aacgtcgtcg atatcagata cggacaggcc
     5521 agcatcctgc agtgcaactt tcagcggctc aatggaacgg tttaccagat cttcaaccag
     5581 gctttccagt ttcgcacgag tcactttgat gttcatgtgt ttcggaccgg tcgcgtctgc
     5641 agtgatgtat ggcaggttaa cgtcggtctg ctgagcggaa gacagttcga ttttcgcttt
     5701 ttctgccgct tctttcaggc gctgcattgc cagcggatcg ttgcgcaggt caatgccctg
     5761 atctttcttg aattcttcaa ccagatagtt gatcagacgg ctgtcgaagt cttcaccacc
     5821 caggtgggta tcaccgttgg ttgccagaac ttcgaaggtt ttttcgccgt caacttcgtc
     5881 gatttcgata atagaaatat cgaaagtacc accccccagg tcataaaccg cgatagtacg
     5941 gttgccagtg cctttgtcca gaccgtaagc cagcgcagct gcggtcggtt cgttgatgat
     6001 acgttttact tccagaccag cgatacggcc tgcgtctttg gttgcctgac gctgagcatc
     6061 gttaaagtat gccggtacgg tgataacagc ttcagttacc ggttcaccca ggtaatcttc
     6121 agcggttttc ttcatttttt tcagcacttc agcagaaatc tgcggcggtg ccattttctg
     6181 gcctttaact tcgacccatg cgtcgccgtt atcagcagca ataattttga acggcatgat
     6241 ggaaacatca cgctgtactt cttcgtcctg gaagcggcgg ccaatcaggc gtttaatcgc
     6301 aaacagggtg ttttgcgggt tcgtcactgc ctgacgttta gccggctgac caaccagagt
     6361 ttcaccatcc tgggtatagg caatgataga aggcgtggtg cgatcgcctt cggcgttctc
     6421 cagcacgcga ggaatggtgc catccataat cgctacacaa gagttggtag tacccaggtc
     6481 gataccaatt attttaccca tctaaacgtc tccactatat attcggtcat catgtggttg
     6541 tgagtctgta ataggggcga aacgtctggt ttcaactgcc caatttcatt tttttgcaga
     6601 ctcactcact gcggttgact actaaatggg gtcgtaaccc acgtcatcaa gggggagatg
     6661 caaaaatttt ttgtgctttt gcgcagaaat ttcgcgatat ggcaggacaa gacgtaaaat
     6721 tgacagcaat tttattcata taaagaatga atcgcagcca aggcattcat caaaaaattg
     6781 taataaaaag aaaagattac gtgcctgaat cttctcttta tcagcagtaa actagtgggt
     6841 attcatcccc ctacctcttc ccactaagag aatccttatg aaatccgttt ttacgatttc
     6901 cgccagcctg gcgattagcc tgatgctgtg ctgcacggcg caggcaaacg accataaact
     6961 cctcggcgtc attgcaatgc cgcgtaacga aaccaacgat ctggcgctga aacttcctgt
     7021 ttgtcgcatt gtgaaacgca tacaactctc tgccgaccat ggcgatttac agttaagcgg
     7081 tgcatcggtt tatttcaaag ccgcccgtag cgccagtcag agcctgaata ttccttcaga
     7141 aataaaagaa gggcaaacca ctgactggat caacattaac agcgataacg acaataaacg
     7201 ctgcgtctca aaaatcacct tttccggtca tacggtgaac tcatcggata tggccacgct
     7261 gaaaattatc ggcgacgact aacgccagaa tatagaagcc acaaaaaatg aatgttaatt
     7321 acctgaatga ttcagatctg gattttcttc agcattgtag tgaggaacag ttggcaaatt
     7381 tcgcccgatt gctcacccat aatgaaaaag gcaaaactcg cctctccagc gtactgatgc
     7441 gcaacgaact gtttaaatcg atggaagggc atcccgagca acatcgccgc aactggcagc
     7501 tgattgccgg agaattacag cattttggtg gcgatagtat cgccaacaaa ctgcgcggac
     7561 acggtaaatt gtatcgggcc attttgctcg atgtttcaaa gcgattgaag ctgaaagccg
     7621 acaaagagat gtctacgttt gaaattgagc aacagttact ggaacaattt ctgcgtaata
     7681 cctggaagaa aatggacgag gaacataagc aggagtttct gcacgcggtc gatgccaggg
     7741 tgaatgagct ggaagagctg ctgccgctgc tgatgaaaga caaattattg gcaaaaggtg
     7801 tgtcgcattt gctttccagc caactgaccc gcattttacg cacccacgca gcaatgagcg
     7861 tacttgggca tggtttgctg cgcggagcgg ggctgggagg ccctgtgggc gcagcattaa
     7921 atggggttaa agcggtcagc ggcagcgcct atcgcgtgac gattccagcc gtactgcaaa
     7981 tcgcctgcct gcgccggatg gttagcgcca ctcaggtctg ataccggaaa ccggggaaaa
     8041 ttttcatttt cccctcaaaa gatcgtagac actgccccac tggctgatta ttatgccgcg
     8101 ccctgaaaac actacagtta ttcagggaaa ttatttcacc attcattcga tgatgatttt
     8161 tgaggaatta tgggcaacac taagttggct aatccggcac cgctgggcct gatgggcttc
     8221 ggcatgacca ccattctgct taacctgcac aacgtgggtt atttcgctct ggacggtatt
     8281 attcttgcca tgggcatttt ctacggcggc atcgcgcaaa tttttgctgg tctgctggag
     8341 tacaaaaaag gcaacacttt cggtttaacc gcattcacct cttacggttc tttctggctg
     8401 acgctggttg cgattctgct gatgccgaaa ctgggtctga ccgatgcgcc aaatgcacag
     8461 ttccttggtg tctacctggg tctgtggggc gtatttacgc tgtttatgtt cttcggcact
     8521 ctgaaaggcg cacgcgtcct gcaattcgtt ttctttagcc tgaccgtgct gtttgccctg
     8581 ctggcgatcg gtaacattgc cggtaatgcc gcgatcatcc actttgccgg ctggattggt
     8641 ctgatctgcg gtgccagcgc aatctatctg gcgatgggtg aagtgctgaa cgagcagttt
     8701 ggtcgcaccg ttctgccgat tggtgaatcc cactaattgt cttgagatcc ccccgctcgg
     8761 ggggattttt ttattcgcta acgtcgcgtc ttgcactctt cggtctgaat gcagcaacca
     8821 cttctggtgc cgtttcaacg tatggccctt ccagcaactg aatgcagtac ggtacgctgg
     8881 caaaaatacc atgcaccaca acgttaccct cagcgtcctt cacaccttcc agcgtctctt
     8941 taatagactt cggctgaccg ggtaagttaa ggatcagcgc ctgtttacga atcacgccca
     9001 cctgacgcga aaggatcgca gttggtacaa aatgcaggct gatctggcgc atctgttcac
     9061 caaagccagg catctcgcgg tccgctactg ccagcgtcgc atcgggcgtt acgtcacgac
     9121 gcgccgggcc agttccgccc gtggtgagca ccagatggca actcatttca tccaccagct
     9181 cacacaacgt ttgctcgatg atcgcctgct catcggggat taagcgggtt tccagttcaa
     9241 acggcgtggt tagcgccgat gtcagccatt cttccagcgc tggaatgcct ttatcctgat
     9301 aaacgccgct ggatgcgcga tcagagatgg aaactaagcc aatacgtaaa gtattcatca
     9361 agatttttcc gtttaaacag tctcgttaaa cagaatgata cactgcgaag ggagtgacag
     9421 acaggcttca gaagaggtag cgtgaccgac ttcccggtca cgctaagaat gattacagca
     9481 gatcgccgat cattttttcc agtttttcct ggtcaatagc aaacttacgg ataccttccg
     9541 ccagtttatc tactgccatt ggatcctggt tgtgctgcca caggaactcg gactcagtga
     9601 tacgcgccgg acgcgctttc acttcgccgg tgtaagacag tttacgttcg atagcccctt
     9661 cgctctccgc cagctctttc agcagtgccg gtgcgatggt cagacggtca cagcctgcca
     9721 gttccagaat ttcgccgatg ttacggaagc ttgcgcccat aaccacggtt tcataaccgt
     9781 gctctttgta gtactggtag atttcagata cagaaaccac gcccggatct tctgccggag
     9841 cgtactcttt cttatcggta ttcgctttgt accagtcaag aatacggcca acaaacggcg
     9901 agatcaggaa cacgcccgcc tccgcacaag cacgagcctg agcgaaggag aacagcaggg
     9961 tcaggttaca gttgatacct tctttttcca gctgttctgc agcacggata ccctgccagg
    10021 tagaagccag tttgatcaga atacgatcgt tgctaatacc tgcatcgttg tagagtttga
    10081 tcaggcgttt tgctttcgca attgacgctt cggtgtcata ggaaagacgc gcatcaactt
    10141 cagttgagat acggcccgga accagtttca ggatttccag accaatattt actgccagtt
    10201 tgtcggtcgc gtccacgatc tgctgcgcgc gatcgttgct ctgctgtttc gcccaggcga
    10261 cagcatcatc aatcaactta cggtattccg gaatctgcgc tgcgttaaga atgagagaag
    10321 ggttggttgt ggcatcctgc ggttgataca gcttcattgc cgcgatgtcc ccagtgtcgg
    10381 ccactacggt ggtgtactga cgaagggagg tcaatttgtc cgtcatgata gtatttctct
    10441 ttaaacagct tgttaggggg atgtaaccgg tctgccctga tgatatcacg acgcaacgtc
    10501 agcgcaaccg ccgacaagac ggttatcgcc aggcgagact gtttcggatt tctgacactg
    10561 cgttgatatc gcccgccatt tttatacaaa acctcatgta tgctacgcag aagttatcaa
    10621 gtacctcgta gcgtatatac ttcttaaaca attggtaacg tttacacagg aaagtcatcg
    10681 cgaccggcaa taagagggat atgcatgcca gattttttct cctttattaa cagcgtcctt
    10741 tggggatcgg taatgattta cctgctcttc ggcgcaggtt gttggttcac ttttcgcacc
    10801 ggatttgtgc agtttcgcta catccgccag tttggcaaaa gtcttaaaaa tagcattcat
    10861 ccacagccag gcggtttaac ctcatttcag tcattgtgta ccagtcttgc ggcgcgcgtg
    10921 ggtagcggca acctggccgc cgttgcgctg gcaattaccg ccggtggacc aggtgccgtc
    10981 ttttggatgt gggttgctgc gtttatcggc atggcgacct cgtttgccga atgttccctt
    11041 gcacaacttt ataaagaacg tgacgtcaat gggcagtttc gtggcggacc ggcatggtat
    11101 atggcgcgcg ggctggggat gcgctggatg ggcgttctgt tcgccgtctt tttgctcatc
    11161 gcctacggca taattttcag cggagttcag gcgaacgccg ttgcccgagc cctgagtttt
    11221 tcttttgatt ttcctccgct ggtgacgggc attattctcg ctgtctttgc tctgctggca
    11281 atcactcgcg gtcttcatgg cgtcgcccgg ctcatgcagg gttttgtccc gttgatggcg
    11341 ataatctggg tactgaccag cctggtgatt tgcgtaataa atatcgggca acttccccac
    11401 gtcatttggt ctatttttga gagtgctttt ggctggcagg aagcggcagg cggcgcggcg
    11461 ggatatacct taagccaggc gattactaac ggttttcagc gcagtatgtt ttccaatgag
    11521 gcgggaatgg gatcgacgcc aaacgcggca gcggcagcgg cagcggcagc ggcagcggca
    11581 gcggcagcgg cagcggcagc tgcatcctgg cctccgcatc cggcagcaca agggattgtc
    11641 cagatgattg gcatttttat cgacaccctg gtcatctgta cggcaagcgc catgctgata
    11701 ttactggcgg gtaacggcac aacctacatg ccgctggaag gtattcagct tatccagaag
    11761 gcgatgcggg tgctaatggg ttcctagggt gctgaatttg ttaccctcgt ggttattctg
    11821 tttgccttca gctccatcgt tgccaactac atttatgccg aaaacaatct cttcttttta
    11881 cgcctgaaca accctaaagc gatctggtgt ttgcggatct gcaccttcgc aacggtcatc
    11941 ggcggcacct tgctaagcct tccgctgatg tggcaactgg cagatatcat aatggcctgc
    12001 atggctatta ccaatttgac cgccatttta ctgctctcgc ctgtggttca taccattgcc
    12061 agtgattatc tacgccagcg taaactcggc gtgcgcccgg tgtttgatcc gttgcgttat
    12121 ccggatatcg gtcgccagct ttctccggac gcgtgggatg atgtttcgca ggagtaatca
    12181 caactatcga tcaactcatt ctcttttttt gctaaagtcg gcataaattt cctgcaagga
    12241 ctggatatgc tgattcttat ttcacctgcg aaaacgcttg attaccaaag cccgttgacc
    12301 accacgcgct atacgctgcc ggagctgtta gacaattccc agcagttgat ccatgaagcg
    12361 cggaaactga cgcctccgca gattagcacg ctgatgcgca tcagcgacaa actggcgggt
    12421 atcaacgccg ctcgctttca tgactggcag ccagatttca cgccggcgaa tgcccgccag
    12481 gcgattctgg cgtttaaagg tgatgtctac accggcttgc aggccgaaac cttcagcgaa
    12541 gacgatttcg attttgccca acagcatttg cgaatgcttt ccggcttgta tggcgtactg
    12601 cgcccgctcg atttaatgca gccttatcgt ctggaaatgg ggatccgtct tgagaatgcc
    12661 cgagggaaag atctgtatca attctgggga gatatcatca ccaacaagct gaacgaggcg
    12721 ctcgcagcac aaggcgataa tgtggtgatt aatctggcgt cagatgagta ttttaaatcg
    12781 gtgaagccga agaaattgaa tgccgagatt atcaagccgg tgttcctcga tgagaagaac
    12841 ggcaaattta agatcatcag cttctacgct aagaaagcac gcggtctgat gagtcgtttc
    12901 attattgaaa atcggctgac caaaccagag caactgactg gttttaatag cgaaggttac
    12961 ttctttgatg aagattcctc cagcaatggc gaactggtgt ttaaacgcta cgagcagcgt
    13021 taagtttgta gtcaatgccg gatgcggcgt gaacgcctta tccggcctac aaaagcatgc
    13081 agattcaata tattgcagaa ttaacgtagg cctgataaga cgcgccagcg tcgcatcagg
    13141 cgctgaatgc cggattgtta cccggcattt gtcatttagc ggtgatgttt gcctggacca
    13201 tgaccgccgt gatgatcatg aggagctttc ttatggtggc gcggcggtgg cggcggtccg
    13261 tgtgggtgcc agcgattgcc tcgccattca taatgttgtt tccaccagcc gtggtcgcgc
    13321 cagtgacctc catcccagta atagccacga ttatcacgat cgcctatctg taattttact
    13381 gacgggacta acgtaatttc cgcagcctgt gctgccatgg gagcgaccag aaccagggaa
    13441 agtgcgagta cgatagattg catctttttc acaggttact ccttttatca ttgcccgtta
    13501 tgggtcgata tccggagatt acgcgatgag aacgacggtt gttattctcc gcaatcctaa
    13561 tctctaagtt accgcattta ttgataattt ctcctttttc cctgtcgttt ttctccataa
    13621 tttcttcata aaaaagccgg gctgcataaa agcaaacccg gcctgattga gataatgaat
    13681 agattttact gatgattcat catcaattta cgcaacgcag aaaaatcggc gggcagatta
    13741 tgtgaaagca agggtaaatc agcacgttct gccagctctt ttggcagatc caacgtttca
    13801 ccgagaatcg cttccacgct ctctttaaat ttcgccggat gcgcggtgcc gaggaacaag
    13861 ccatattcgc ctggattcaa ctggtcacgc agcgcacgat acgctacggc agcgtgcggc
    13921 tccgaggtgt agcccagttc ttttaactca cgcattgtct gttgcgtggt ttcatcatcc
    13981 acggctgcat aacccagctc tttcagttgc cagattttgc ggcggaacaa ctcttccaca
    14041 cgcggccagt tgttcggctg actcacgtcc atcgcgttgg ataacgtcgc ctgagtcgct
    14101 ttgggtgacc actgaccgtc gtgcaggaaa cgtggcacgg tatcgttcac gttggtcgca
    14161 gcaataaaac gtttcaccgg cagaccgaat gacttcgcca gcagacccgc cgtcaaatcg
    14221 ccgaagtttc cgcttggcac cgagacaacc agctggttgc gcgcctcctg cggcagctgc
    14281 gcaacagctt caaagtagta gcaaatctgc gccagcaaac ggctgatgtt aatcgagtta
    14341 gccgagttta accccagcgc cactttcagt tcttcatcat caaacgcctg cttcaccagc
    14401 gcctgacagg catcgaaatc gccgtcgatg gcaacagttt cgatattgcc gcccaacgta
    14461 cagaacagtt tttcttgcag tggactgatt ttgcctcgtg gatagaggat aaccactttc
    14521 acattcggca aaccgtagaa agcatgagcc actgcagctc cggtatcacc ggaggtcgcg
    14581 gtcagaatgg tcactggctt atcatccgca atatgggtca gcatttgtgc cataaagcga
    14641 ccgccgaaat ctttaaatgc cagcgttggc ccgtggaaca attccagaca accgacatcg
    14701 ctttcaacat tggcgaccgg agccgggaag gcaaacgccg cgcgcacgcg ctcttccagg
    14761 atttcctgtg ggatttcatc accaataaac gccgagagga tcttcgcact gcgggtgaca
    14821 aaatccagct tcagcatctc atcaatttca gtcaggctga attccggcag gtcgtgcgga
    14881 aaaaacagcc cctgattttt gcccaacccc tgggttacgg cttgcgcaaa gctgacctgc
    14941 tcgttgtgat ctttcagatt gtagagtttc atttagtttt ccagtactcg tgcgcccgcc
    15001 gtatccagcc ggcaaatatg aacaaaacct tcctgatttt gcaggtagtt cttacccaac
    15061 cagtcggcaa cgcgctgggc ggtatccggc ttgtcacata gagcgaacaa ggtcgggccg
    15121 gagccggaga taccgctcgc taccgcgccg atttccgcga ccgcctgccg cgcctgccgg
    15181 aagccaggca gtaaccgttc acggtagggt tcagcgataa catctttcat cagcttcgcg
    15241 gcaagctcag gctgacggga atagcaggcg tgaatgaagc cagccagatg tcgcccgtgc
    15301 gcaatgcaat cctggcggcg atactgcgcc ggtaaaatag cccgggcttc tgccgtcgag
    15361 actttaattc ccggatacgc cagcacccac agccactcat caaaccctgg cacttgctgg
    15421 ctgatgatgt cgttttcttc gatcatcaac tgcataccac caagaaaaca cggtgccacg
    15481 ttgtcgtaat gaatgctgcc ggagatacgt ccttccagct cgcccatcaa agccagcaaa
    15541 cgagtgtcat taagcggctt gccgcagtgt tcattcatcg ccatcagcgc cgcgaccacc
    15601 gaacaggcgc tggagcctaa gcccgaaccg atcggcatat tcttttccag agtcatcgcc
    15661 actggaattt gcttgccaag ctcctggcaa aaacgctccc agcactgata aacgatattt
    15721 tcccgtggtt ctgacggcag cttatcggca aagcgtccga ggttgttgag actgaatgtc
    15781 tctgccgcct caaccgtgac tacatctccg agcaatgcac catcaacggg tgccaccgcc
    15841 gccccgagca catcaaaccc gacgctcata ttggcactgg aagccggggc ataaacttta
    15901 accatgtcag actcctaact tccatgagag ggtacgtagc agatcggcaa agacaccggc
    15961 agcggtaacg tcattgcccg caccatatcc gcgcagcacc aacggcagcg gctgataata
    16021 gtggctataa aaggccaggg cgttttcgcc atttttcact ttgaacagcg gatcattccc
    16081 atccacttcg gcaatcttca cgcggcagac accatcttca tcaatattgc caacatagcg
    16141 caaaactttt ccttcatcac gggccttcgc cacgcgcgcg gcaaagagat cgtcgagctg
    16201 tgacagattc gccataaaag cggcaacatc accctcggcg ttaaactctg cgggcagcac
    16261 aggttcaatt tcaatatccg ccagctccag ttcacgtccc gtttcacgag cgagaatcaa
    16321 tagtttacgc gccacatcca taccagaaag atcatctcgc gggtccggtt cggtataacc
    16381 catttcccgc gccagcgtgg tcgcctcgga gaaactcatg ccttcgtcta acttgccgaa
    16441 gatataagaa agcgaacctg aaagaatgcc ggagaacttc accaattcat cacctgcatt
    16501 gagcagattt tgcaggttct caataaccgg taatccagcc ccaacgttgg tgtcatagag
    16561 gaatttacgc cgcgattttt ccgccgcata acgcaactga tgatagtaat ccatcgacga
    16621 ggtgttggcc tttttgttcg gcgtgacaac gtggaaacct tcgcgcagga agtcggcata
    16681 ttgatccgcc actgcctggc tggaggtgca gtcaacaatg accgggttca gcagatgata
    16741 ttctttcacg aggcgaatta agcgcccgag attaaacggc tctttggctt gtgcaagttc
    16801 ttcctgccag ttttccagat taaggccatg cacactagtg agcagagcct tcgagttggc
    16861 aacgccgcag acacgtaagt cgatatgttt attcttcagc cagctttgct gacgcttcag
    16921 ttgctccagc agcgcaccgc caacgccacc gacgccaatc acaaacactt cgataacctg
    16981 atcggtattg aacagcatct gatgagtaac gcgcacgcca gtggtcgcat catcgttatt
    17041 taccacgaca gagattgagc gttcagaaga tccctgagca atggcgacaa tgttgatatt
    17101 ggcgcgggcc agcgcggcaa agaatttcgc cgagatccca cgcaaggtgc gcataccatc
    17161 acctacaacc gagataatgg ccagccgttc cgtcaccgcc agcggctcca gtaagccttc
    17221 tttcagttcc aggtagaact cttcctgcat tgcccgttca gctcgcacac agtcgctttg
    17281 tggaacgcag aaactgatac tgtattcgga agatgattgc gtaatcagca ccacggaaat
    17341 acgggcgcgt gacattgcag caaagacgcg cgccgccatg ccaaccatcc ctttcatccc
    17401 cgggccggaa acgctgaaca ttgccatgtt attcagattg gaaatgccct tgaccggtaa
    17461 ttcgtcttca tcacggctgg caccaatgag cgtacctggt gcttgaggat ttccggtatt
    17521 tttaatcagg caagggatct ggaactgggc gatgggggta atggtgcggg ggtgaagaac
    17581 tttagcgccg aagtaggaaa gctccatcgc ttcctggtag gacatcgact tcaacaacct
    17641 cgcatcgggc acctgacgcg ggtcgcaggt atagaccccg tcaacgtccg tccaaatctc
    17701 gcaacaatcg gcgcgtaaac aggcagccag caccgccgcg gagtagtcgg aaccgttacg
    17761 tccaagcacc accagttcgc ctttttcatt accggcggtg aaacctgcca tcaacaccat
    17821 gtgatcagcc ggaatgcggc ttgccgcaat acggcgggtg gactcagcaa tatcgacggt
    17881 agattcgagg taatgcccca ctgccagcag tttttcgacc ggatcgataa cggtaacgtt
    17941 gtgaccacgc gcttctaaca cgccggccat aatggcgatc gacattttct cgccacggca
    18001 aatcagcgca gcgttgatgc tatccgggca ctgccccaac aaactaatgc catgcaggac
    18061 atgttttatt tgggcaaatt cctgatcgac gaaagttttc aattgcgcca gcgggaaccc
    18121 cggctgggcg gcggcgagtc ccgtcaaaag ttcggcaaaa atacgttcgg catcgctgat
    18181 attgggtaaa gcatcctggc cgctaatggt tttttcaatc atcgccacca ggtggttggt
    18241 gattttggcg ggggcagaga ggacggtggc cacctgcccc tgcctggcat tgctttccag
    18301 aatatcggca acacgcagaa aacgttctgc atttgccact gatgtaccgc cgaacttcaa
    18361 cactcgcatg gttgttacct cgttaccttt ggtcgaaaaa aaagcccgca ctgtcaggtg
    18421 cgggcttttt tctgtgtttc ctgtacgcgt cagcccgcac cgttacctgt ggtaatggtg
    18481 atggtggtgg taatggtggt gctaatgcgt ttcatggatg ttgtgtactc tgtaattttt
    18541 atctgtctgt gcgctatgcc tatattggtt aaagtattta gtgacctaag tcaataaaat
    18601 tttaatttac tcacggcagg taaccagttc agaagctgct atcagacact ctttttttaa
    18661 tccacacaga gacatattgc ccgttgcagt cagaatgaaa agctgaaaaa tacttactaa
    18721 ggcgtttttt atttggtgat atttttttca atatcatgca gcaaacggtg caacattgcc
    18781 gtgtctcgtt gctctaaaag ccccaggcgt tgctgtaacc agtcgaccag ttttatgtca
    18841 tctgccactg ccagagtcgt cagcaatgcc atgactcgtt cgcgtaaagc ttgcagttga
    18901 tgttggtctg ccgttgtgtc acttttcgcc ggttgttgta ttaatgttgc taattgatag
    18961 caatagacca tcaccgcctg ccccagattg agcgaaggat aatccgccac catcggcaca
    19021 ccagtaagca cgtcagccaa cgctaactct tcgttagtca gcccggaatc ttcgcgacca
    19081 aacaccagcg cggcatggct catccatgaa gatttttcct ctaacagcgg caccagttct
    19141 actggcgtgg cgtagtaatg atatttcgcc cgactacgcg cagtggtggc gacagtgaaa
    19201 tcgacatcgt gtaacgattc agccagtgtc gggaaaactt taatattatc aataatatca
    19261 ctagatccat gtgcgaccca gcgggtggct ggctccaggt gtgcctgact atcgacaatc
    19321 cgcagttcgc taaaacccat cgttttcatt gcccgcgccg ctgccccaat attttctgct
    19381 ctggcgggtg cgaccaggat aatcgttata cgcatattgc cactcttctt gaccaaataa
    19441 ccgcgaacag ggtgatcact gtcaacttat tacgcggtgc gaatttacaa attcttaacg
    19501 taagccgcag aaaaagccct ttacttagct taaaaaaggc taaactattt tctgactgca
    19561 ctaacggttt aattgttaaa aaatgctaca tatccttctg tttacttagg ataattttat
    19621 aaaaaataaa tctcgacaat tggattcacc acgtttatta gttgtatgat gcaactagtt
    19681 ggattattaa aataatgtga cgaaagctag catttagata cgatgatttc atcaaactgt
    19741 taacgtgcta caattgaact tgatatatgt caacgaagcg tagttttatt gggtgtccgg
    19801 cccctcttag cctgttatgt tgctgttaaa atggttagga tgacagccgt ttttgacact
    19861 gtcgggtcct gagggaaagt acccacgacc aagctaatga tgttgttgac gttgatggaa
    19921 agtgcatcaa gaacgcaatt acgtacttta gtcatgttac gccgatcatg ttaatttgca
    19981 gcatgcatca ggcaggtcag ggacttttgt acttcctgtt tcgatttagt tggcaattta
    20041 ggtagcaaac atgcagaccc cgcacattct tatcgttgaa gacgagttgg taacacgcaa
    20101 cacgttgaaa agtattttcg aagcggaagg ctatgatgtt ttcgaagcga cagatggcgc
    20161 ggaaatgcat cagatcctct ctgaatatga catcaacctg gtgatcatgg atatcaatct
    20221 gccgggtaag aacggtcttc tgttagcgcg tgaactgcgc gagcaggcga atgttgcgtt
    20281 gatgttcctg actggccgtg acaacgaagt cgataaaatt ctcggcctcg aaatcggtgc
    20341 agatgactac atcaccaaac cgttcaaccc gcgtgaactg acgattcgtg cacgcaacct
    20401 gctgtcccgt accatgaatc tgggtactgt cagcgaagaa cgtcgtagcg ttgaaagcta
    20461 caagttcaat ggttgggaac tggacatcaa cagccgttcg ttgatcggcc ctgatggcga
    20521 gcagtacaag ctgccgcgca gcgagttccg cgccatgctt cacttctgtg aaaacccagg
    20581 caaaattcag tctcgtgctg aactgctgaa gaaaatgacc ggccgtgagc tgaaaccgca
    20641 cgaccgtact gtagacgtga cgatccgccg tattcgtaaa catttcgaat ctacgccgga
    20701 tacgccggaa atcatcgcca ccattcacgg tgaaggttat cgcttctgcg gtgatctgga
    20761 agattaatcg gctttaccac cgtcaaaaaa acggcgcttt ttagcgccgt ttttattttt
    20821 caaccttatt tccagatacg taattcatcg tccgttgtaa cttctttact ggctttcatt
    20881 ttcggcagtg aaaacgcata ccagtcgata ttacgagtca caaacatcat gccggccagc
    20941 gccaccacca gcacactggt tcccaacaac agcgcgctat cggcagagtt gagcagtccc
    21001 cacatcactc catccagcaa caacagcgcg agggtaaaca acatgctatt gcgccagcct
    21061 ttcaataccg cctgcaaata aataccgttc attaacgccc caatcagact ggcgattatc
    21121 catgccacgg taaaaccgat atgttcagaa agcgccagca agagcaaata aaacatcacc
    21181 aatgaaagcc ccaccagcaa atattgcatt gggtgtaaac gttgcgcggt gagcgtttca
    21241 aaaacaaaga acgccataaa agtcagtgca atcagcagaa tggcgtactt agtcgcccgg
    21301 tccgttaatt ggtattgatc ggctggcgtc gtcactgcga cgctaaacgc ggggaagttt
    21361 tcccagccgg tatcattgcc tgaagcaaaa cgctcaccga gattattagc aaaccagctg
    21421 ctttgccatt gcgcctgaaa acctgactcg ctagcttccc gtttggctgg tagaaaatca
    21481 cctaaaaaac tgggatgcgg ccagttgctg gttaaggtca tttcgctatt acgcccggca
    21541 ggcaccacag aaagatcgcc ggtaccgctt aaattcagtg ccatattcag ctgcaggttc
    21601 tgcttccgcc agtccccttc gggtaaaggg atatgcacgc cctgcccgcc ttgctctaac
    21661 ccggtaccgg gttcaatggt cagtgccgtt ccgttaactt caggcgcttt caccacacca
    21721 ataccacgcg catccccgac gctaatcaca ataaagggct tgcctaagat gatatttggc
    21781 gcgttgagct cgctaagacg cgaaacatcg aaatcggctt ttagcgttaa atcactgtgc
    21841 cagacctgac cggtataaat ccctatcttg cgttcttcca cgttctgatt gccatcaacc
    21901 atcaatgact caggtaacca aaaatggata aaacttcgtt tccgctccac ggttttatcc
    21961 tcttcctgca ccgtataaag ctcggtcaca ggaatagcga taagcggccc aacgagtttt
    22021 tgcggcccgc tggtactttg gcgaatcgca tcttccacat cgctacggta atcggcacgt
    22081 tcgacaatca cttgccgaat cagcattatc ggaatcaaca acagcaatac tgcaccaaac
    22141 aaggtggtca ttttccagaa caggggggat ttcaacatag tcttctcctt tgcaatggca
    22201 gcaggatacg aaggctatgt gggaagaatt tgaagctatg tgaagttacg gtgaagtcgt
    22261 agcgaggcca gcacgccacc ttcctgcacg ttgcgcagcg tgacttcgcc gttaaacaaa
    22321 cgggcgacct cactgacgaa cgccaacccc agaccgctgc ttttttgccc atttgcacgc
    22381 ggcaaagagt aaaagcgttc aaaaatacgt gacagcgcgt agtcaggaat accactaccg
    22441 gtatccagca ccttaagcgt gacgtgttcc tgatccactt cggcgcttag cgttatgcaa
    22501 ccgctctcgg gagtaaaatc gatggtgtta tccagtaaat tccccagcgc ctgatccagt
    22561 aacgccggtt cagcagcaac attaacctcg gtgggcataa catgcaaagt gatgtttttt
    22621 tctgccaact gcacggtgcg cgcttcgctg acgcggcgaa ataatgccgc cacatcaaca
    22681 gcagtcagaa cgacttcctg acgattctcc agtcttgcct ggcgtagtaa cgtttccacc
    22741 agcgcctgca ttcgcgcatt ttgtgtcaga atgttgtcag taaaacgagc cactacttca
    22801 ggcggcggac cttcgcgtaa aatttccgcc gcgccacgaa tcgccgccag tgggcttttt
    22861 agctcatggg ttaacgcata aacatactgc tcaatatagt ttttcccttc cagcttcacg
    22921 cgcatacttt ccagcgcctg cgcgagttta cgcagctcgc tactaccgag atcgggaaga
    22981 ggaacgggct tattgtcagt gacggaatca gcatagcgag tgagccgggc aatggagcgg
    23041 ttgatccacc aaaccatgcc tgcgccaatc accagtgcaa tccccaacaa aatggcgctg
    23101 gcccataaaa ttcgccgctc gctgcgttta atgaccggag ccatcgccgc gttcggtttg
    23161 cctacgctca aaacgccaat aagccgcgag ccgcccataa tcggcgcggc aacatacatc
    23221 accgaacttt cgggatcggc aggattttgc aacgtgctgc gcgcaccata ctgaccacgc
    23281 aacgttagcc agacgtcatt ccagcgcgaa taatcctgtc caacggcttt atttgccgaa
    23341 tcgaacaata ctttgccatg cgcatcggtc atatagacat gatattcatt gcgcactttg
    23401 ttaatgccac caatattggc gcgaaacggg cgatgttgta gctgattaaa cgcctgcgcc
    23461 agttgcccat gcgttgggtc cccagagagc aaatcgggac gcgccagctc cgcaagtaac
    23521 gttgccgtgt cgatcaacgt cccctccgtt gctcttcgca cgcccggttt aacttctttg
    23581 acaaaaatag ccagtacgaa ccaggccgcc accgccacca gtaaaaaata gcccagcagc
    23641 aaccgcatgc cgatacgcat tacaagcccc tcaggctata tcccatgccg cgatgagtat
    23701 taatcggtga aagatcgggg ttgatggcgc gcagcttggc acgcagcgtt ttaatgtgga
    23761 tatcgacggt gcgatcgtag gtgtcctgcg catcttccca cacgctatcc attagttgct
    23821 ggcgggacca gacgcggcct ggcgacttga gtaacgtctt cagcaataaa aactcatacc
    23881 gcgtcagcgt taatggcgtg tcaaaccagc tgatctgcgc cgcgggttca ttcaattcaa
    23941 aatgaccaat acggatgacg ggagacggcg tcgagaactt cttcacccga cgcagtaagg
    24001 tgcgcaccct ggcgcacact tcgcggggtg aaaacggttt agccacatag tcgtcaacgc
    24061 caatttccag cccaagcagg cgatcgacct cttcacttcg ggccgtcagg aacagtacag
    24121 gtaacgccgg atggagcgcc agtaactggc ggcacaattc aaagccactg atatcaggca
    24181 aaccaacgtc gagaatcatg acgtcgggaa cctgctggcg agctttatcc agcaccggca
    24241 aacctcgctc aaagacctcg acggcaaaac cttcctgctg caacatgtag accagcgtgt
    24301 cggctatccc ttgctcatct tccactaacc agaccgtttc ccgttgcata aatcgcctct
    24361 gttattgccg ccacggcatg acaggaaccg cgctaatcgc gtttttcggc gaaccttcta
    24421 caactttgtc ggagtaagcc agataagcga gcgcgttgcg tttggcgtca taaaagcgca
    24481 cgacctgtaa cgacttaaag accagggacg tgcgtttttt gaatactacc tcgccctgag
    24541 ctttgccgtt tttaatacga tccgacagtt caatcggccc gacttgctga caagaaatgg
    24601 ccgcatcgga ggtatcttct gccaggccca atcccccttt aataccaccg gttttcgccc
    24661 ggctcacata acaggtgaca tttttcacat cgggatcgtc aaaggcttcc acaacaattt
    24721 tgtgatccgg gccgatcatt ttaaatacgg tgtcgaccga accaatctct tctgcatgag
    24781 ccaatggccc cagcattatc aggcttaaag acaggatcaa atgcttgtat ttcatattgt
    24841 taccattttt atctattaca cgagcaatta gttcgtcacg gtaaataaca tattgattaa
    24901 gaacaaaatc tcaattcttt tatttccgtc aggccctaaa acatactcta ctaagaaaaa
    24961 aacactgaat gctaaaacag caaaaaatgc tattatccaa ttacctgatg tcaggtgctc
    25021 gttgttgaaa ggatgaggat attttatgga tcaggccggc attattcgcg accttttaat
    25081 ctggctggaa ggtcatctgg atcagcccct gtcgctcgac aatgtagcgg cgaaagcagg
    25141 ttattccaag tggcacttac agagaatgtt taaagatgtc actggccatg ctattggcgc
    25201 gtatattcgt gctcgtcgtt tgtcgaaatc ggcggtcgca ctacgcctga ctgcgcgtcc
    25261 gattctggac atcgcgctgc aataccgctt cgactctcaa cagacattta cccgcgcatt
    25321 caagaagcag tttgcccaga ctcctgcact ttaccgccgt tctcctgaat ggagcgcctt
    25381 tggtattcgc ccgccgctac gtctgggtaa attcacaatg ccagagcaca aatttgtcac
    25441 cctggaagat acgccgctga ttggtgttac ccagagctac tcctgttcgc tggagcaaat
    25501 ctctgatttc cgccatgaaa tgcgttatca gttctggcac gattttctcg gcaacgcgcc
    25561 gaccattccg ccggtgctct acggcctgaa tgaaacgcgt ccgagtcagg ataaagacga
    25621 cgaacaagag gtattctata ccaccgcgtt agcccaggat caggcagatg gctatgtact
    25681 gacggggcat ccggtgatgc tgcagggcgg cgaatatgtg atgtttacct atgaaggtct
    25741 gggaaccggc gtgcaggagt ttatcctgac ggtatacgga acgtgcatgc caatgctcaa
    25801 tctgacgcgc cgtaaaggtc aggatattga gcgatactac ccggcagaag acgccaaagc
    25861 gggagatcgc ccaattaatc tgcgctgtga actgctgatt cctatccgcc gttaacgctg
    25921 cagctcatct aatgcagggg cgtccagatg cgagatgtcc cctgcggttt caacgaccca
    25981 gccagacgcc agccacaggc tttcctgata atccacgcgc gaaatagaac agttacgcag
    26041 acgtaagcgg cgctctgccc atgctggtaa tccgagaatc gtactcacca gacatcccag
    26101 tgcaatacca tggctcacca gcagcggtcg gcttccctgc ggtaagtccc ggcaagattc
    26161 cagtgccgca ttgacgcgat cgctgagctc ctgcattgac tcgccttcag gaatacgccc
    26221 gtcaacggtg ccattgacca gctgccgacg ccagttctct tcttcttcgg tcagagaatc
    26281 gatatgtctt ttttccagca cacccatgtt taattcacgc aggcgagaat caaagatgat
    26341 gtcacagccg caggcctggg cgatgatttc cgccgtacgc cgggtgcgtc ctaaatcgct
    26401 actgataata tgcgtaatgc caagctcttt ggcacgggtt gccacctgca tcgcctgttg
    26461 ctcacctttg gcggtcagcg ggctgtcaga ctggccctga atacgtcgct cggcgttcca
    26521 ctgcgtttca ccgtggcgga ctaggtatac ctgtaacatg ctttttttcc gtatactgct
    26581 gtaacgttaa ttttctgagt aatgctgatt atgcaccaag ttgtctgtgc gaccaccaat
    26641 cccgctaaaa ttcaggccat tctgcaggca tttcacgaga tcttcggcga aggatcctgc
    26701 catattgcat ccgttgccgt cgagagcggt gtaccggaac aaccctttgg cagtgaggaa
    26761 acgcgcgctg gcgcacgaaa tcgggtagca aatgcccgcc gtttacttcc agaggctgat
    26821 ttttgggtgg cgattgaagc tggcattgat ggcgacagca ctttcagttg ggtggtgatt
    26881 gaaaacgcca gccagcgcgg cgaggcgcgt tcggcaactt tgccgttacc tgcggtgatt
    26941 ctggaaaaag ttcgtgaagg cgaggcgctt ggtcccgtga tgtcgcgtta taccggtatt
    27001 gatgagattg gtcgtaagga aggggcgatc ggcgtgttta ccgccgggaa actcactcgc
    27061 gccagcgtct atcaccaggc ggtgatcctg gcgctgagtc cgtttcacaa tgccgtgtat
    27121 caggcattgt aggacggata aggcgttcac gccgcatccg acattcggtg cacgatgcct
    27181 gatgcgacgc tgccgcgtct tatcaggcct acaaaatcaa tcgcttttca gcaacacctc
    27241 ttccagccac tgacgcagct ccacgggcgc ggctttcagg ctgttagatc cacgcgtaat
    27301 cgtcgcgatg cccgcgccga gttcattttt taactcacgc tgactcattt cgccacgcaa
    27361 cagctcttcg acaatacgca cgcgagtccc caacgcttcg cgctcatctg gcgtcagcat
    27421 caggtttaac aacggtaaat ggagatcgtt ttggtaggca ttcttaagca ggtcgacaaa
    27481 acgtaaccac tcctggtgac gctgttctgc catcgctgct gaatagggtg attgttgggc
    27541 cataatatgt cgccattgtt agcgggggaa gcaaaatgac tcccccggtt gtactcgcta
    27601 aagagtacga tagcatatca taaacgtgcg gatcagtaac gacgtcccca ttccgtggcg
    27661 ctcatcaacg tcggtttatc ccccatgaaa tagcggtagt aagcatcata agccagcacg
    27721 ttcttcacat aaccgcgcgt ctcggagaac ggaatactct cgacaaatgc cacagcgtcg
    27781 atacgcccgg cgctgttgcc aagccaggtt cgcacccgtc caggtccggc gttataagct
    27841 gctgaggaga aaatacggtt attgccaaac tgctgataaa catattgcag gtaactggtg
    27901 ccaatgttga tgttcgtttc cggatccagc aattgcccag ggctgctata accaggaata
    27961 gagaacatct tcaccgtatg ggtcgctgta ccaggcataa tctgcatcag gccgctggcc
    28021 cctaccggtg atttcacttt cggattccag gcgctctcct gacgggcaat cgccatcgca
    28081 tagctttgcg ggatctcttt cccactggtg taacgtttga aaagatcgtt gtaagccagc
    28141 gggaatcgct cttccagatg atcccaaagc ttcccggcga tcgttgcctg aacgctaaga
    28201 tcccaccatt ggttattgaa agcataccga gccagttgag cctgctctgt ttttgacttg
    28261 ctcttcacca gattggccca ctcgctacgc gcggtattat cgagattcca gtacatcaac
    28321 tcgcgcacgc gcgccatctc cggcccctga gtcagggtgc tgtcaacatt ctgcggcgct
    28381 ttatcaatct tcagctcata ctcttcaccg atgcgttgtg ctgcaaccat cgggtagaaa
    28441 ccacgctgtt gcatgagttg atgcaaaatc tctttcgctt cagcttcacg tccgcgttcc
    28501 agcaataaat ccgcctgcca gtaacgccat tcatctttct cttttgcttc catcggcaga
    28561 cgcgccagcc aggtattcag gccgcgacga tcgccggtgc caagcgccat tcgcacacgg
    28621 cgttcaatca acgacgtgga ttgcgagcgc ataatggcgt catcgcgcca tttcgcctgc
    28681 tcatcggtga catcattgcc catcaaacgc caggcgacga tatcgcgcag ctcctgaatc
    28741 tgatcttcat taagctgctg cgcctgggca agcgacggga tcatcagccg tgcattttcg
    28801 gcatcctgcc gcgccacact ggcaaacgcc accgccgcca tttgacgggt aaaatcggtc
    28861 gcgccggttg tacgcgcgaa ggtcagtacc gtattagggt tgttcgccag tgaaatgatt
    28921 gccgaggcga tagtctggta atcggcaggc atctgccctg ccagcactgt taccaggcct
    28981 gtgttacccg ctttcatcgc cagacggata cgctctaaat acgccagcgg atcttgttta
    29041 cctgacgcac gccagacgct aaataacttg tcacaggcgt taggctggct cttgccggtt
    29101 agccacagct ctttcgcccc ttgccaggct tcttcactct gcccggtgtt ccatttcgca
    29161 tagtagtaat tacattgcgc ttcggtagtt ccgggctttt ccgggctaaa ggctaacaag
    29221 ccacgccagt cttcacgccg cgccagttca ttgacgaaac gagattgcag cgtgcgagcg
    29281 ggaggaagcg tggggttagc gcgaacaaag ttagtgaccg tcaccgccgg ttgattcatc
    29341 aggtcatcgg tgatctggcg gtattccagg tagggataaa gcggataatc cttcagtcca
    29401 ggcatcattt gttcgaccac atccatctgt cgattatccc aggcctgctt gatttgcgcg
    29461 taacggctac gctgctcatc cagtgagtcg gctcgcgcca cgctgctgac cgtcagcaga
    29521 cagacaccgg cagccaacag ccgccaggta acttgtttgg ctttttccac aagcgcatcc
    29581 tctaagtgta aatacatcaa tgcagcgtca taccgcgtaa tagatttatg ctaaccagtc
    29641 attgccgttt acgccacgtt acggacactt ttttactttt actgcgaagg tgatcgacca
    29701 actgggatta ggcggggaaa caggctacac ttcgcctttg aaaactcatc atcacgataa
    29761 acatagaggc gaagtccaac gtggctcaat tcgtttatac catgcatcgt gtcggcaaag
    29821 ttgttccgcc gaaacgtcat attttgaaaa acatctctct gagtttcttc cctggggcaa
    29881 aaattggtgt cctgggtctg aacggcgcgg gtaagtccac cctgctgcgc attatggcgg
    29941 gcattgataa agacatcgaa ggtgaagcgc gtccgcagcc agacatcaag attggttatc
    30001 tgccgcagga accgcagctg aacccggaac acaccgtgcg tgagtccatt gaagaagcgg
    30061 tttcagaagt ggttaacgcc ctgaaacgcc tggatgaagt gtatgcgctg tacgccgatc
    30121 cggatgccga ttttgacaag ctggccgctg aacaaggccg tctggaagag atcattcagg
    30181 ctcacgacgg tcataacctg aacgtacagc tggagcgtgc ggcggatgcg ctgcgtctgc
    30241 cagattggga cgcgaaaatc gctaacctct ccggtggtga gcgtcgtcgc gtggcgttgt
    30301 gccgcctgct gctggaaaaa ccagacatgc tgctgctcga cgaaccgacc aaccacctgg
    30361 atgccgaatc cgtggcgtgg ctggaacgct tcctgcacga cttcgaaggc accgtggtgg
    30421 cgattactca cgaccgttac ttcctcgata acgttgcagg ctggatcctc gaactggacc
    30481 gcggtgaagg tattccgtgg gaaggtaact actcctcctg gctggatcag aaagatcagc
    30541 gtctggcgca ggaagcttca caagaagcgg cgcgtcgtaa gtcgatcgag aaagagctgg
    30601 agtgggtacg tcagggaact aaaggcagcc agtcgaaagg taaagcacgt ctggcacgct
    30661 ttgaagagct gaacagcacc gaataccaga aacgtaacga aaccaacgaa ctgtttattc
    30721 cacctggacc gcgtctgggc gataaagtgc tggaagtcag caacctgcgt aaatcctacg
    30781 gcgatcgcct gctgattgat gacctgagct tctcgatccc gaaaggggcg atcgtcggga
    30841 tcatcggtcc gaacggcgcg ggtaaatcga ccctgttccg tatgatctcc ggtcaggaac
    30901 agccggacag cggcaccatc actttgggtg aaacggtgaa actggcatcg gttgatcagt
    30961 tccgtgactc aatggataac agcaaaaccg tttgggaaga agtttccggc gggctggata
    31021 ttatgaagat cggcaacacc gagatgccaa gccgcgccta cgttggccgc tttaacttta
    31081 aaggggttga tcagggtaaa cgtgttggcg aactttccgg tggtgagcgc ggtcgtctgc
    31141 atctggcgaa gctgctgcag gttggcggca acatgctgct gctcgacgaa ccaaccaacg
    31201 acctggatat cgaaaccctg cgcgcgctgg aaaacgccct gctggagttc ccgggctgtg
    31261 cgatggttat ctcgcacgac cgttggttcc tcgaccgtat cgccacgcac atcctggact
    31321 accaggatga aggtaaagtt gagttcttcg aaggtaactt tactgagtac gaagagtaca
    31381 aaaaacgcac gctgggcgca gacgcgctgg agccgaagcg tatcaagtac aagcgtattg
    31441 cgaagtaatg cgtaaaatgc cggatgcggc gcgaacgcct tatccggcct acaaaaccag
    31501 caatttcaat acgttgtaaa actgtaggcc tgataagacg cggtaagcgt cgcatcaggc
    31561 attgagcacc gaatgccgga tgcggcgtga acaccttttc cggtctacaa cagccttagc
    31621 atcctcaatt tcgtggcggg tcacccgtgc aggttttttc aaaccttgtt taatctcgac
    31681 agcctcttcc agagaggttt tcaactcatc aaaaaaactc attttatacc tcgcctttga
    31741 gcagtttcgt cacggttatc cttgctcccc catcatctcc cgcaccagct ccacgcagcg
    31801 caggaaacgg ctgtcgtaat cgtcctcttc aacccgcacg aattcgatat tgttctcttc
    31861 cagcatctcc accagcaagt tctggaactc tttgcgatcc accgaactgc cgaggctgcg
    31921 taaaccatcc gccacccacg gcgtgttgtt ctccagcagg atcaccagat cgaaacggta
    31981 ttcatcaatc agcgcctgca cgaacggatg ctcacgccct tcgtactttt tgcagaacgc
    32041 ctgggtagtg acaaaatcgg tgtcaataaa cgccacttta ttggcgtatt tcactgcaaa
    32101 atcaatgtat tgtgcgtggc ccaacgcgat tttatcgtag tcggaatact gcaatgcgat
    32161 ctcatcaccg ccgaggtgtg aaaagacata atcgcggcca tattcccacg cactggtggt
    32221 gttgaagata ttggcaagtt tgtttaccag ggtggattta ccgctcgact cgccgccaag
    32281 gatcgccacg gtacgcacaa aaaacggctt cacttcggta ggaatatatt cccagtagcg
    32341 gaacgggttt tcgcggatct gcgcaccgct gatactcata aaggtacgtt tcggatcaac
    32401 cagcaccgtc tcgatcccca gatgttccat atactgtggc gcatcggctt cttccgaggt
    32461 gtagatcagg tccggctgga tccccttttc agccataaac tttttgatgc cgttgctcca
    32521 cacatcccag ccatgcggat acggctccat gccctcttcg ttgaaagcat gaatacggat
    32581 atttttctga tatttaaagg tttgcaatag ccagcgcaga cgatccggca ccgtgggctg
    32641 ctgcgacatg gcgctgtctt caaacaacgc gcggtcgcgg gtatcgtcaa aacccataat
    32701 gatatgcagc tcgtcaacct ggctacaggc gcgctggata aggtagatat gtccggtatg
    32761 cagcgggtag aacttaccga atacgacacc aatggttttc ttctgccggg gaaattcaag
    32821 ccccaaaaaa cggtgcaatg cctccagctt ttgcgcgctg gggcttttaa ttttggcatt
    32881 cagtaactgg cttaaatacc ctttggtcat accgctggca tcagctacct gctgtagcgt
    32941 gcagccctgt tgcttgatgg cagttttcag gtaatcaaat gacgacatat ctccctccgt
    33001 atctctcatt ataagtcgtc gaacacgcta agcgcgtcgg agagtttttt aacgccaaaa
    33061 atctgcatcc cttccggcgc ttttttcggt acgttagccg ccggaacaat cgcccggcga
    33121 aaaccgtgtt tcgccgcttc agaaatccgc tcctggccac tgggcaccgg gcggatctcc
    33181 cctgccagcc cgacttcacc aaacaccacc agatcctgtg gcagcggtct gtcacgcagg
    33241 ctggaaacca tcgccagcag taacgctaag tcggcgctgg tttcggttac cttcacgccg
    33301 ccgaccacgt tcacaaacac atcctgatcg gccatttgca gaccaccgtg acggtgcaag
    33361 acagccagca ggattgccag acggttttgt tccagcccca ctgccacgcg gcgcggattc
    33421 gccattatcg agtgatcgac cagcgcctga atctccacca gcaacggacg cgttccttcc
    33481 cacaccacca tcacggagct gccggaagtc acttcatctc cgcgacttaa gaaaattgcc
    33541 gaagggttgc tgacttcacg cagcccctgt tcggtcatcg cgaagacgcc cagctcattc
    33601 accgcgccga agcggttttt atggctgcgt aaggtgcgaa aacgggagtc ggcatcgcca
    33661 tccagaagca ccgaacagtc gatacagtgt tccagcactt tcgggccagc cagcgaacca
    33721 tctttggtta cgtgtccaac catgacaatc gccacaccgc gcgttttggc gaaacgcgtc
    33781 agataagccg ccgtttcacg cacctgcgcc acgctaccag gcgatgattg tacatccgcc
    33841 atatgcatca cctggatcga gtcaattacc atcagcttcg gttgctcttc ttcggcaatc
    33901 aggcagatct gctcgatgct ggtttccgac aacatattga gattgtcagt cggcaggcca
    33961 aggcgatgag cgcgcattgc cacctgttgc agcgactctt cgccggtgac atacagcgtt
    34021 ttcatctgct gggccagttt gcacagcgtt tgcagcaaca gcgtggattt ccccgcacca
    34081 gggttaccgc caatcagaat ggcacttcct ggcaccacgc cgccgcctag tacgcggtcg
    34141 aactctttaa atccggtgga aaaacgcggc agctcttcaa ggctgatatc ggagagtttc
    34201 tggacttttg ccaccccggc gctaccggca tagccgctga gacgctcgtt acgcgccacc
    34261 gttggcgacg cagcaagacg cacctcggtg atggtgttcc aggcatgaca ggcactgcac
    34321 tgcccctgcc agcgcggata atcggccccg cattcattac aaacaaaggc gcgttttgga
    34381 gcttttgcca cggtgtacct cgttaatgct gtgccgcccg caggatgacg ggcaggcaat
    34441 tacttctgat tcaggctgcc agagaggatg cagaataccc ccatcaggtc agcgtgacgg
    34501 atggtgactt ccgccttttc attcactttt ggcttggcat ggtaggcaat ccccagccct
    34561 gctgctttga tcatcggcag gtcattagct ccatcgccaa tcgccacggt ctgcgccagc
    34621 gggatttcat actcctgcgc gaggcgagtc agagttttcg ctttgtactg tgcgtctacg
    34681 atgtcgccga tcacattgcc ggtaaattta ccgtccatga tctccagttc attggctagc
    34741 acggctgtca ggcgcagctt gtcgcgcagg tattcagcaa agaaagtaaa gccgccggag
    34801 gcaatcgcca ctttccagcc cagcgtttcc agcttgagca ccagctgcgt taagcctggc
    34861 atcagcggca gattttcacg cacctgttgc agaatattgg cgtcagcgcc tttcagcgtc
    34921 gccacgcggc tgcgcaggct ggcggtaaaa tcgagttcgc cgcgcatcgc ccgttcggtt
    34981 acctccgcta ccatctcgcc cgttccggcc agcttggcaa tttcatcaat acattcaatc
    35041 tggatggcgg tggaatccat gtccatcacc agcaaacccg gcgtgcgcag gtgcgggatt
    35101 ttccccagcg gggcgacatc cagctgcgct tcgtgggcca ggcgtgtagc ccgtgcggtg
    35161 agtgaacctg ccagacgaat cacctgataa tcttccacgc accaggcggc aacaatcacc
    35221 atcgccgcac ccagtttgct ctggtattgg gtcagacgct gtttatccag cccacgacca
    35281 tacagcagcc agccgctacg agctgcgtgg taatccagtg gcatcacttc atcaccactt
    35341 aatgaaagag gcagacccgg ccataaagag acatcttcag gcaggtcgca ccaggtaatg
    35401 ttaggcatta aggctcctgt aaaatcgttc gaagcaggga aaataacgca tgaggctacc
    35461 ttgtatccat tgcttctggc aacattaagt ctcaaatttt caaagggtgg aagatggctc
    35521 gcacaaaact gaaattccgg ctacatcggg cagtgattgt cctgttctgt cttgccttgt
    35581 tagtggcact gatgcaggga gcgtcatggt ttagtcaaaa ccaccagcga cagcgtaatc
    35641 cacagctgga agaactggcc cgcaccctgg cgcgtcaggt gacgctgaac gttgcaccgc
    35701 tgatgcgtac cgactcaccg gatgaaaaac gcattcaggc gatcctcgat cagttaacgg
    35761 atgaaagccg tatcctcgac gcgggtgtgt atgacgaaca aggcgatctt atcgcacgtt
    35821 ctggcgaaag cgtcgaagtg cgcgatcggc tggcgctcga cggtaaaaaa gcaggcggct
    35881 attttaacca gcagattgtc gagccaattg cgggtaaaaa cggaccgctc ggctatctgc
    35941 gcctgacact cgacacccat acgctcgcca ccgaagccca acaggtggat aacaccacta
    36001 acattttacg cctgatgttg ctgctctcac tggcaatcgg cgtagtgctg acccgcacgc
    36061 agctacaggg taaacgcacc cgctggcagc aatcgccctt cctgttaacc gccagcaaac
    36121 cggtgccgga agaggaagaa agcgagaaaa aagagtgacc cattactaca agaaaggaaa
    36181 tcgttatgtc cacattacgc ctgctcatct ctgactctta cgacccgtgg tttaacctgg
    36241 cggtggaaga gtgtattttt cgccaaatgc ccgccacgca gcgcgttctg tttctctggc
    36301 gcaatgccga cacggtagta attggtcgcg cgcagaaccc gtggaaagag tgtaataccc
    36361 ggcggatgga agaagataac gtccgcctgg cgcggcgcag tagcggtggc ggcgcggtgt
    36421 tccacgatct cggcaatacc tgctttacct ttatggctgg caagccggag tacgataaaa
    36481 ctatctccac gtcgattgtg ctcaatgcgc tgaacgcgct cggcgtcagc gccgaagcgt
    36541 ccggacgtaa cgatctggtg gtgaaaaccg tcgaaggcga ccgcaaagtc tcaggctcgg
    36601 cctatcgcga aaccaaagat cgcggcttcc accacggcac cttgctactc aatgccgacc
    36661 tcagccgcct ggcaaactat ctcaatccgg ataaaaagaa actggcggcg aaaggcatta
    36721 cttcagtgcg ttcccgcgtg accaacctca ccgagctgct gccggggctc acccatgagc
    36781 aggtttgcga ggccataacc gaggcctttt tcgcccatta tggcgagcac gtggaagcgg
    36841 aaatcatctc cccggacaaa acgccagact tgccaaactt cgccgaaacc tttgcccgtc
    36901 agagtagctg agagtggaac ttcggtcagg ctccggcatt ctcgcatctg ctggatgaac
    36961 gctttagctg gggcggcgtg gaactgcatt tcgacgttga aaaaggccat atcacccgcg
    37021 cccaggtatt taccgacagc ctcaaccccg cgccgctgga agccctcgcc gggcgactgc
    37081 aaggctgcct gtaccgcgcg gatatgctgc aacaagagtg cgaagcgctg ttggttgact
    37141 tcccggacca ggaaaaagag ctacgggagt tgtcgacgtg gatagcgggg gcggtaaggt
    37201 aactcgctga ttaaaatatc caaggaagga aatatgatcg atctggaaaa ccaggaacgg
    37261 gagattatca atttaatgtt cagccagcgt atttcctggc tagccgccgt gcgtatcagg
    37321 cataaattat cgcttgctga agtaagtaaa atgttaggta tctcgattaa ttctctaaaa
    37381 cagatcgaaa agacggaacg gcttagctca aacatcaaaa gcaaaatggc agaaatttat
    37441 ggttgtccac cagagttgct gatatgccct tcctggatga cggctgaaca taaatgaata
    37501 gattcgtgaa tagatacttg aatgtatttt attttctatc catatgaaaa ataatagtaa
    37561 atctataaat cagttgctat tgatgaatag atactaaaaa gccggagcag tcccccactc
    37621 cggcttaaca cgacaatcgc ctttcagcga aacacaatta ctctttatcg cccagcagaa
    37681 cggattccag tgcgattttg atcatgtcgt tgaaggtggt ctgacgctca gcggcagtgg
    37741 tctgctcgtg agtgcggatg tggtcagaca cggtgcagat ggtcagggct ttcgcgccaa
    37801 actctgcagc cacgccgtag ataccagccg cttccatttc cacgccgagg atgccgtatt
    37861 tttccatcac gtcgaacatt tcgccgtccg gagagtagaa caggtcagcg gagaacaggt
    37921 tacccacgcg agcatcaacg cccagtgctt tagctgcatc tactgcgtta cgcaccatgt
    37981 caaagtcagc gatagcggca aagtcatggt ctttaaaacg gatgcggtta actttggaat
    38041 cggtgcaggc ccccataccg ataacgacgt cgcgcagttt tacgtgcggc agaactgcgc
    38101 cacaggaacc aacgcggata attttcttca cgccgaaatc ggtgatcagt tctttggtgt
    38161 agatggagca ggacgggata cccataccgt gacccattac ggaaattttg cggcctttgt
    38221 aagtaccagt gaagcccagc ataccgcgaa cgttgttcac ttcacgggca tcttcaagga
    38281 aagtttcagc aatatacttc gcacgcagcg ggtcgcctgg catcaaaact acgtcagcga
    38341 aatcgcccat ttctgcatta atgtgtgggg tagccattgt tttatccttt ttgtgacata
    38401 acaaaggcgg agtcaacgct ccgcccaaat ccatcagaac atggctttgc catattccat
    38461 atcagaagta ccaaaatatt ttgccagagt ctggccgata tccgcgaagg tttcacggtg
    38521 acccagtgag cccggtttta ctttcgggcc atataccagt accggaatgt gttcacgcgt
    38581 gtggtcagta ccggtccagg tcggatcgca accgtggtca gcggtgagga tcaggatgtc
    38641 gtcatcgcgc agcagagaca tcagctccgg cagacggcgg tcgaacagtt ccagacccgc
    38701 agcataaccg gcgacgtcgc gacggtggcc ccaggaagag tcgaagtcaa cgaagttggt
    38761 gaagacgatg gtgttatcac ccgcttcttt catctctttg atggtggcgt caaacagcgc
    38821 gtccaagcca gtcgctttca cttttttggt gataccgcag ttggcgtaga tgtctgcaat
    38881 tttaccgaca gaaaccacct ggccgtgttt ttcatcaacc agtttctgca gcacggtcgg
    38941 tgctggcggc tcaacagcca ggtcgtgacg gttaccggta cgctggaagt taccagcttt
    39001 gtcaccgata aacggacgag cgataacacg accgatattg tagccgccgt tggtcagctc
    39061 ttcacgggcg atttcgcaca gttcgtagag tttatccaga ccgaaagttt cttcatggca
    39121 ggcaatctgg aacacggagt cagcggaggt atagaaaatc ggcttgccgg ttttcatgtg
    39181 ctcttcgccc agttgatcca gaatgaccgt accggaagag tggcagttac cgaggtaacc
    39241 cggcagatta gcgcgttcga ccagtttatc cagcagctct tgcgggaagc tgttttcgtg
    39301 atcggagaaa tatccccact caaacagaac cgggacaccg gcgatttccc agtgaccaga
    39361 cggggtatct ttaccggatg acatttcgtg cgcccatgcg tacgcgccga taacttcagc
    39421 gttgccgtcc attcccgccg gaatgaaacc ggtagaacct tcgtgtgctt tcgccagccc
    39481 cagacggatc agatttggca gattgagcgg ccctttacga ccattatcag cttcgccttt
    39541 ggcacaagct tctgcgatat gacccagggt gtcagccccg acgtcaccaa agcgttctgc
    39601 atcttctgta gcgccgatgc cgaatgagtc cagcaccata ataaatgcac gtttcatatg
    39661 ttctccgtac tcagtgcttc aaatttaaat gatcagatca gtataccgtt attcgctgat
    39721 acggcgatag acagttggtg tgctttccgg tgcgttatcg gcaagtttaa ttgccgcttt
    39781 caccgctttc gccgcttcct gccagctgtt ttcgtctttc gcgaggataa ccgccagcgg
    39841 acgctgaccg tctacatggt cgcccagacg cgccatatca gtaaagccga cgctgtaatc
    39901 gatggtgtca gatgcctgac gacgaccgcc gcccattgca accactgcca tccccagcgc
    39961 gcgggtatcc atttcactga caaaaccttc ggtatcagca tagactgctt tcgtcagcat
    40021 cgctgtcggc agatacttcg cgtagttctc aacgaagtcg gtcgggcctt tttgtgccgc
    40081 taccatacga ccaaagactt ctgccgcttt accgttgtcc agcaccgcct gcaatttcgc
    40141 gcgcgcttcg gcgtcatctt tcgccagttt gccggagatc agcatctcca cgcacagcac
    40201 catcgtgaca tcaaacagac gcgggttacg atactcaccc gtcaggaact gcaccgcttc
    40261 acgaacttca accgcgttac ctgcactgga ggccagtacc tgattcatgt cggtgagcag
    40321 cgcggtggtg cgcacgccag cgccgttagc cacgccaaca atcgcttcgg caagggcttc
    40381 agagagttcg taggtcggca taaacgcgcc gctacccact ttcacgtcca tcaccagcgc
    40441 gtccagacct tccgccagtt tcttcgccag gatcgaggca gtgatcagcg ggatggagtc
    40501 cacggttgcg gtaatatcac gggtcgcgta gaaacgttta tcagccggag ccagtgaact
    40561 ggtctgaccg ataatcgcca cgccgacgtc tttaataatt tcgcggaaac ggttgtcatc
    40621 cgggaaaatg tcgaagccag ggatggattc cagtttgtcg agcgtaccgc cagtatgacc
    40681 gaggccgcga ccggagatca tcggaatata gccgccgcag gctgcgacca tcggccccaa
    40741 catcagcgaa gtgacatcgc cgacgccgcc ggtcgagtgt ttatcaacaa tcgggccatt
    40801 cagatgcagg cttttccagt cgagaacggt tcctgaatct cgcatcgcca tggtcagcga
    40861 gacacgctca ggcattgtca tatcgtggaa gaaaatggtc atcgcgaggg cggcaatctg
    40921 cccttcggaa atagtgttgt cgcgaatacc gttgataaag aaacgaattt cttcatcgct
    40981 cagcgcatga ccatcacgtt ttttacgaat aatttcttgt gcgagaaaca aggtaacctc
    41041 ccgaggaaaa gagtatgagg cggcttttgc cggatgcgtc tacgccttat ccggcctacg
    41101 ggtagatacg tctcgtaggc ctgataagcg cagcgcatca ggcgtaaagc atcttactta
    41161 gtagctgctg gcgctcttac cgtcgccgtg gcccagcgct ttcagcaggc ttgccagcag
    41221 gctggaagca ccaaagcggt agtgacgcgc atcagcccag tcagcaccga acagttcatc
    41281 tgcgatggcg agatatttct gcgcatcttc cgcagtacgc acgccgcccg ccggtttgaa
    41341 accaacggtt ttttctacgc ccatataacg gatcacttcc atcatgatgc gagcgctttc
    41401 cggcgtcgcg ttcacagcca ctttaccggt agaggttttg atgaagtccg cacccgcttt
    41461 gatggagatt tcagacgctt tacggatcag cgcttcgtct ttcagttcgc cggtttcgat
    41521 gatcactttc agcagtacat tcgctgccgc gcaagcctct ttacaggctt tcaccaggtc
    41581 aaaaccaacc tgctcgtcac ccgccatcag cgcgcggtac gggaacacca cgtcaacttc
    41641 atcagcaccg taggcgattg ccgcacgggt ttctgccagc gcgatttcga tgtcgtcgtt
    41701 accgtgtggg aagttggtta ccgtagcgat acggatttcc ggggtgccct gctctttcag
    41761 agttttgcga gcaatcggga taaaacgagg atagatacag atagcggcgg tattgccgac
    41821 cggagttttg gcctgatgac acagagcaat tactttctcg tcggtgtcgt cgtcattcag
    41881 ggtggtcagg tccatcaatt tcagtgcacg caggctgctt gctttcagat cagtcatttc
    41941 attctcctgg cttgtcgcca ataaaattca ccttgcgagt ttgttagtat tctaacatct
    42001 actccgcaac acacttcgat acacatcaca attaaggaaa tctacttaca agtttgcatc
    42061 actgtaatgc gatctggttc aaataattca ctttcaaatg aatgcgtcag tggtggcaaa
    42121 cgcatcagga tcaaagtgaa catcacgaaa cttcttacaa tggcgcatca ccttcggcat
    42181 aaaaggaaaa gaagatgccc acttctcatg aaaatgcact gcaacaacgt tgccagcaaa
    42241 ttgtcaccag cccagtgctt agcccggagc agaagcgcca ttttctggca ctggaagcag
    42301 aaaacaatct gccttaccca cagcttcctg ccgaagcccg ccgcgcgctg gatgaaggtg
    42361 taatctgcga tatgtttgaa ggtcatgcgc cgtacaaacc gcgctatgtc ttacccgatt
    42421 acgctcgttt tctggcgaac ggttccgaat ggctggagct ggaaggcgcg aaagatcttg
    42481 atgacgcact ctctctgctg accattcttt accaccacgt accgtcggtc acatcgatgc
    42541 cggtctacct ggggcaactg gatgcgttgt tgcaaccgta tgttagaatt ctaacacaaa
    42601 acgagatcga tgttcgaata aaacgtttct ggcgttacct cgacagaacc ctgccagatg
    42661 cctttatgca cgccaatatc ggcccgtctg attcgcccat tacccgcgcg atcttacgtg
    42721 cagacgcgca ggtttcaccg aacctgacct ttatctacga tcctgaaatc acccctgatg
    42781 acctgctgct ggaagtggcg aagaacatct gtgaatgtag caaaccgcac atcgccaacg
    42841 gtccggtgca tgataaaatt ttcacaaaag ggggctacgg gattgtgagc tgttacaact
    42901 cactgccgct ggcgggtggt ggcagcacgc tggtacgcct taacctgaaa gccattgccg
    42961 agcgcagcga atcgctggat gacttcttta cgcgcactct accgcactac tgccagcagc
    43021 agatcgccat catcgatgcg cggtgtgaat tcctctatca acaatcacac ttctttgaga
    43081 atagcttcct ggtgaaagaa gggctgatta accctgaacg ttttgtgcca atgtttggca
    43141 tgtatgggct ggcggaagcg gttaacttgc tgtgtgagaa agaagggatt gccgcgcgct
    43201 acggtaaaga agccgccgca aatgaagtgg gttatcgcat cagcgcgcaa ctggcggagt
    43261 ttgtcgccaa tacccccgtg aaatatggct ggcaaaaacg cgccatgtta cacgcacagt
    43321 cggggatcag ttccgatatc ggcaccacgc cgggcgcgcg tttgccgtat ggcgatgagc
    43381 cagatccgat cacccatctg caaactgtcg ccccgcatca tgcttattat tattccggca
    43441 tcagcgacat tctgacgctc gacgaaacca tcaaacgtaa cccgcaggca ctggtacagc
    43501 tttgcctcgg tgcctttaaa gccggaatgc gtgaatttac cgccaatgtc agcggtaacg
    43561 atctggttcg cgttaccggt tatatggtgc gtttgtcgga tttagaaaaa tatcgcgccg
    43621 aaggttcacg caccaacacc acctggctgg gcgaagaagc cgcacgcaac actcgtattc
    43681 tggaacgcca gccgcgcgtg ataagccatg aacagcagat gcgctttagt ctgtaagatt
    43741 atccccttct cctgcgttga cgggccaggc agtcgtctgg ctctgttttt gcagggctgc
    43801 aatctgcgct gcaaaaactg tcacaatccg tggacgatgg gacgttgcaa tgactgtggg
    43861 gagtgcgtgc cacagtgtcc gcatcaggcg ttgcagattg ttgacggcaa agtggtgtgg
    43921 aacgctgtgg tctgcgagca atgtgatacc tgcctgaaga tgtgtccgca acatgccaca
    43981 ccaatggcgc aatccatgag cgtggacgaa gtgcttagcc atgtgcgcaa agcggtgctg
    44041 tttattgaag ggataacggt aagcggcggc gaagtcacga cccaactgcc gtttgtagtg
    44101 gcgctgttta ctgctatcaa aaacgatccg caactgcgcc atctcacctg tctggtggac
    44161 agtaacggta tgttgagcga aaccggctgg gaaaaattac tcccggtgtg tgacggcgca
    44221 atgctcgatc tcaaagcatg gggtagcgaa tgtcatcaac aattaaccgg acgcgataat
    44281 caacagatta agcgcagcat ctatttgctg gcagagcgcg gcaagctggc ggaactgcgt
    44341 ttgctgatga tcccaggcca ggtggattat ttgcaacaca tcgaagaact ggcggcgttt
    44401 atcaagggac ttggagatgt tccggttcgc ctgaacgcgt ttcatgccca cggcgtgtat
    44461 ggcgaggcgc aaagctgggc gagcgccacg ccggaagacg ttgagccgtt ggctgatgca
    44521 ttaaaggtgc gcggggtgag ccggttgata tttccggcgc tctatttgtg acatgccgga
    44581 tacggcctac ggttcggcac agacttgtag gcctgataag acgcgtcaag cgtcgcatca
    44641 ggcattgtgc gccaactgcc ggatgcggcg tgaacgcctt atccggccta cggttcggca
    44701 caaacctgta ggcctgataa gacgcgttaa gcgtcgcatc aggcattatg cgccgactgc
    44761 cggatgctgc ctacgaaggg caaactaccc acgaacgtta aacaccgcat acgtgttatt
    44821 aagcaacact tcggcaatct catccgcggg ttccgggcgt aactcgcaaa gcacggcaaa
    44881 cacacgggca gcctgttccg ggcggttagg ctgcccctga aaaccgttga gcggcatatc
    44941 cggcgcatcg gtttccagca acaacgatgc cagcggtaat tttgcgatga catcgcgggt
    45001 tttactggcg cgtggatagg tgatagtacc gcctacgccg attttgtagc ccagctgtac
    45061 aaaccgttca gcctgttgct ggctgccaga aaatccgtgg acaacaccag cacgcggcaa
    45121 atcgtggcgt ttaagatgca tcgccagttt gtcgtgcgtg cgccgtgaat gcaggatcac
    45181 cggcagatcg tagcgtttcg ccagtttcag ttgttcgtcg agtaaccact gctgcctctc
    45241 aaattgcgga tcgtcgccga agagatccag accgatctcc cccaccgcca ccaccttcgc
    45301 cggacgcctt tccagcgcct gctgtagctg ctcaagagac acatcgctat gtttttccaa
    45361 cataccagga tgcaagccca atgcggcata cagcggttga taattttccg ctaacgcctg
    45421 cacgcgggca aaattttccg cctctgtcgc cggaacaatg atcttgccta cgcccgcttg
    45481 tgccgcgcgt tgcaggctgg cctcttcatc gccaataaaa ggcgggaaat caaaatggca
    45541 gtgagtgtcg ataaaacggc aaatcaagcc agatcctcat tgttaaatgt cgggtcgttc
    45601 gcctgcggag cgtcgctcac ttcggcaacc agcgtatcgt tggcgactgg cgcaggcgga
    45661 atgacaatcg attccggcgt caccactggc accagatggc gggtaagcgg cgctttttca
    45721 gttaatagct tgccaaccgt ggcgaggaaa taacgaccgc ataaacgccc aagtttatag
    45781 tcttcacgca gcgccggaat ccgactgcca agcgcgatac tatgtaatgg cttcggcgga
    45841 taaatttcgg atatccgcag cttgcccggt ggtttctcaa taaattgctg aatgtcacga
    45901 tagctggttt catgatgctg caccagattg accagcggct gcaggctact gtcacccagc
    45961 cagcgttcca tgcgtttgaa ccactgcggc gtgtagtaca tttgtgacgg cacagtgcgg
    46021 atgacgacca acgttttagc gccctgcctt gccgcctctt taaccggaat cgcatcactg
    46081 atcccgccat ccaggtagtt aatgccttcc agcgacactc cgctacgata aaagccaggt
    46141 atcgcactgg aggcgcgaat cacatccagc cagttttgtt tggttggtaa aaagtaattc
    46201 ggcgcgtagt catcctgacg acaggcgcac atataaaacg atttgccgct gtcaaacaac
    46261 cgcgcggcgg tatccatttg caacggcatc tggcttgcgg tggcctccac cagccagtcg
    46321 agatcgataa gatttcctcc acggacaaag cgcaatggat cgaaaaattc gcgttttgtg
    46381 gtatagcgca tgatgacttt gcgcgcgtaa ccgggctgat tgcagataaa cgccgagagg
    46441 ttctgcgccc cggcagatgt gccgagataa agatcgaaag gattaaactg cgcgcgcata
    46501 aactcatcca gcacgccagc cgtgaaaatt ccacgctgtc cgccgccttc gcacaccaga
    46561 gctattcgtc caggctggaa cggccttagc gacaacggcg caatattacc aagcgttaca
    46621 ggtattcgct gccccacttc tgccttcctg tttttgttgt gaaagaaaga caaagtaacg
    46681 caatttatta ttcgctaaaa ccgcaaaagc cagtccgctg gactggcttt ggataaaatt
    46741 ttcttaatgg tctgaaatct agggtcgttt tcggcccatg aacaaactca ctaggaacag
    46801 aataatcccg acgacaaaga caattttagc tgcgcctgca gcggtaccgg ccagaccacc
    46861 aaacccaagt gcggcggcga ttaacgcgat aaccagaaat atgatgcccc aacgaaacat
    46921 aagcgtctcc tttaccatag ttaatgtcac cgcttgatat gagcgtgaaa atcactcaat
    46981 gcgatatttt tagtgtggtg cacatcgcgc ctcccgacaa agttcgggag gacgaattac
    47041 gacgaattac ttagttttca gatcattttt aacgcttttc acaccatcta ccgctttggc
    47101 gatactttca gcacggtcac tttgtgcctg agaatctacg gtaccggaga gctgaaccac
    47161 gccatcggtg gtttcaactt tcactttacg tgaagggacg atatcgtccg ccagcagttt
    47221 ggctttgatt tcactggtgg tggcggtgtc acccgcgtag tccttcaccg agccttcttt
    47281 agcgtcgcga acgtgcagtt tgtcgctgac agaggtcacg ccttcaacgc ctttcgccac
    47341 tttcactgcc tcttcggcct gggcctgact ttcaacgaaa ccgctcaggg tcacgacttt
    47401 ttgatcggtt ttgacagaga tatcggtgct cttgatgttg tcatgatcca ccagcgccgc
    47461 cttcactttc gcggtgatgg cgctgtcatc catgaaatta ccgactttat tcatagagct
    47521 atcgactttt tgccctgcgc tttcattggt agtctgcgcg ttgttttccg cgtaggcaga
    47581 gccggtcgcg acggcagagg tcaacattac agccagcaga gttttcgaaa tcttcagtct
    47641 tgtcatagtc atcgatttat tcctgtatgt ttgctcgtaa tttgagccta gcaacacgag
    47701 gttgcattgc tgaataggga gagacttcac cctctacaga agtcaatggt cgccatcaca
    47761 aaagcgatgg gtgatgaata acgaccatta cagtctctga atcagttatt aatatcggta
    47821 gaatgacaat cgacggcttt agatactgat atctacgcat tgaacggtat ttaacgccat
    47881 cagaaatgtc atcactttgt taaatataga tcacaatttt gaaaccgctc gggatatcag
    47941 cgaaaacata agcaaaagtg aatgttttaa gaacattccg taagcggcta ataaggaagg
    48001 gaaattgaca gggcgcagcg gctaccgcgc cctggaggca agaattaatg ctcgcgggtc
    48061 tggtggaact gaacgtccgg ataacgttcc tgtgccaggc gcaggttgac catgctggta
    48121 gcgatgtaag cgaggttatc gccgccatca agcgccagtt ggctttcgtt cttacgcttg
    48181 aactcttcga atttcttcgc gtctgcacat tctacccagc gggcagtggc aacgttgact
    48241 gactcataca ctgcttcaac gttgtattcg ctcttcaggc gcgctaccac cacatcaaac
    48301 tgcagcacac caaccgcacc aacgatcaga tcgttgttgg agattggacg gaacacctgc
    48361 accgcgccct cttcggaaag ctgtaccagc cctttgagca gctgtttttg cttcagcgga
    48421 tctttcaggc ggatacgacg gaacagttct ggtgcgaagt tcggaatacc ggtgaacttc
    48481 atcatctcac cctgggtaaa ggtgtcgccg atctgaatgg tgccgtggtt gtgcaggccg
    48541 aggatatcgc ccggatacgc ttcttcaacg tgcgaacggt cacccgccat aaaggtcagc
    48601 gcgtcggaga tcaccacatc tttcgcagtg cgcacctggc gcagtttcat gcctttttca
    48661 tatttaccgg acaccacacg cataaacgcc acgcggtcgc ggtgtttcgg gtccatgttg
    48721 gcctgaattt taaatacgaa gccggtaaat ttatcttcgc tcgcttctac ggtacgggta
    48781 tcggtctgac gcggcatcgg cgcaggcgcc cactccacca ggccattcag catatgatca
    48841 acgccgaagt tacccagcgc agtaccgaag aataccggag tgatttcgcc cgcaaggaac
    48901 agctctttgt cgaactcgtt agacgcgcct ttaaccagtt ccagttcgtc acgcagctgc
    48961 tgtgccagat cttcaccaac cgcagcatcg agatccgggt tattcagccc tttaacaatg
    49021 cggacttcct gaatggtgtg ccctttaccg ctctgataga gataggtttc atctttataa
    49081 aggtggtaaa cgcctttaaa cagcttgccg cagccaattg gccaggtgat cggtgcgcag
    49141 ccaattttca gctcgttctc aacttcatcg agaagttcca tcgggtcgcg gatatcacgg
    49201 tcaagtttgt tcataaaggt gaggatcggc gtgtcgcgca gacgggtaac ttccatcagc
    49261 ttacgggtac gatcttcaac accttttgcg gcgtcgataa ccatcaggca gcagtccacc
    49321 gccgtcaggg tacgataggt atcttccgag aagtcttcgt gccccggggt gtcgagcagg
    49381 ttaaccaggc aatcgtgata cggaaactgc atcacagacg tagtaatgga gatcccacgc
    49441 tgcttttcca tctccatcca gtccgactta gcgtgctggt tggaaccacg gccttttact
    49501 gtaccggcgg tctgaatggc ctgtccgaac agcagcacct tctcggtgat ggtagtttta
    49561 ccggcgtccg ggtgagaaat aatggcaaaa gtgcggcgct tcgccacctc ttgcaaataa
    49621 ggagacaacg tcataattct ttcttcttga gtaagcggca tcgcgccgcg cttgttgaaa
    49681 acgaaaaatt gcggctattt tacccatcaa cgggggggag gcaatcagtg tttacacagg
    49741 agctgctcca gttcgtgcaa cgaagaaacg gtccaggtgg gcgcgatgcc ttctggttgc
    49801 tcgcgattgt gcgcattcag ccagcaggtc gcaagcccgg cgttgatgcc accgagaata
    49861 tcggactcgg cagtgtcgcc aaccatcagc acgcgtgaac ggtcaggatt gcccgcctgt
    49921 tccagcgcat aatcgaaaat tttcttattc ggtttggcaa cgccaacttc ttcggaaatc
    49981 accagcaaat cgaagtaatc acgcaggccc gtccgttcca gacgcacttg ctgcaaggcg
    50041 ctaaaaccgt tggtgatgat gccgatttta gcgttgccac gaatggcgtt aagcagagaa
    50101 accgcgcccg gcaacggcgt acagatttcc gccatcgcat taataaaggc atcgttaagc
    50161 aagcctgctt caactttcag ccgttcggcc cagctctcga aacgcccgtg ctgaagctgt
    50221 aatgaagtga tcgcgccgtt ttgataatcc acccacagtg gcttgttaac ggcctgatag
    50281 tcctgaaaat cttcagcggt aaaggtgacg ctgtaatcaa gaaacatccg ctgcaggccg
    50341 gtgaatgagt caaaggtaaa cagcgtttca tcggcatcaa agaaaatcca gtcccacttc
    50401 attattccac cttgtcttac atactgattg gcaacgccat gatgatggcg tcttcgcgac
    50461 cgtccgtggt ggggtagtaa ttgcggcgaa tcgtcgcctc gttaaagcct aaactttcgt
    50521 acagggcaat ggcggcagca tttgaggcac ggacttccag ccatagtgtc gccacgccgc
    50581 gtttttccag ttcgtcgatc agatgttcca gcagcgccct acccagcccc tgacgctgat
    50641 agtcgggatc gaccgcaata ttgaacaatg tagcttcatc caacaccact tgcgtaatcg
    50701 caaacgccgc cattttgccg ttttgcgtta actgaaagtt gagataacgc tcgccctggt
    50761 tgctggcaaa ggttttttca ctccacggaa aggcgtgggc gcgttgttca atgtggtaag
    50821 ccgctggtaa atcagtcgtt tcgagggaag aaatcgtgtt catatgtgca aatttgttgc
    50881 cataacgcgg cgcgtgccgt tgggtttacc cgtaattcgg tgagcgccgg tgatgccacc
    50941 tgagcgcctt ccaatgatag cggttcgtca gtacccaacc gccagctgtt gcagcgactg
    51001 ccttgcggca gcatcgcgat tttttctggc gtcagttgca gcacttgatc ggggctgacg
    51061 gttaatgcgc gcagaacatc gctcactaaa ggatcagtca gggcgggaag atcgtttgcc
    51121 accatcacca gacggacgtg tgccgggctc gcaatggcaa tctccccctg caacgcgcca
    51181 gggcgacgca gcgaccactg ggtaatgccc agttgctgta actgccagtc tcgtcgggat
    51241 gtcatagcga atcgctcctg ttgtcagggg cgcaaatata gcaaattcgt cgataccgcg
    51301 ccaacatatg gctataatcg ccgccagtat caattgagga gcattccatg tctgcattta
    51361 ccccggcaag tgaagtcttg ctgcgtcaca gtgatgattt cgaacaaagc cgtattctgt
    51421 ttgccggaga cttacaggat gacctgcccg cgcgtttaga taccgcggcc agccgtgctc
    51481 atacccagca attccaccat tggcaggtgt taagctgcca gatgggggat aacgcccgtt
    51541 ttagtctggt cgccacggcg aatgatgtcg cagattgcga tacgctgatt tactactggc
    51601 cgaagaacaa accggaagcc cagttccagt tgatgaattt actttctctg ctgccagtgg
    51661 ggacagatat ttttgtcgtt ggcgagaacc gcagcggcgt gcgcagcgct gagcagatgc
    51721 tggcagatta tgcgccgttg aataaagtcg acagcgctcg tcgctgtggc ctctattttg
    51781 gtcgtctgga aaaacagcca gtatttgatg cggataaatt ctggggcgaa tacagcgtcg
    51841 atggcctgac ggtcaaaacg ctgcctggcg tgtttagccg cgacggtctg gatgtcggta
    51901 gccagttgct gctctcgacg ttaactccgc acacgaaagg taaagtgctg gatgtcggct
    51961 gtggcgcggg tgtactttca gttgcctttg cgcgccattc gccgaaaatt cgtctcacct
    52021 tgtgcgatgt ctctgccccg gcggtagaag ccagccgcgc aacacttgca gccaacggtg
    52081 ttgaaggtga agtctttgcc agcaacgtct tttccgaggt gaaaggttgt tttgatatga
    52141 tcatctccaa cccgccgttc cacgatggga tgcaaaccag cctggatgcg gcgcaaacgc
    52201 tgattcgtgg cgcggtgcgt catcttaata gcggcggcga gctgcgaatt gtagcgaacg
    52261 ccttcctgcc ttacccggac gtgctggatg agacatttgg cttccatgaa gttatcgcgc
    52321 aaaccgggcg cttcaaggta tatcgcgcca ttatgacccg ccaggcgaag aaaggttgag
    52381 tatctgatat ctgtcgccgc ataccgtgtc ggatgcggcg tgaacgcctt atccgaccta
    52441 caaaacctgc acgctagttt cctgatggac atttttccag caattacaca tcaggcgata
    52501 attaactatt gacgaaaagc tgaaaaccac tagaatgcgc ctccgtggta gcaattcttt
    52561 ttaagaattg atggtatgcg aaggtggcgg aattggtaga cgcgctagct tcaggtgtta
    52621 gtgtccttac ggacgtgggg gttcaagtcc cccccctcgc accaaaaacc acgttgatat
    52681 tgctcgcact gggcgaaggt ggcggaattg gtagacgcgc tagcttcagg tgttagtgtt
    52741 cttacggacg tgggggttca agtccccccc ctcgcaccaa acgaggcgat atcaaaaaaa
    52801 gtaagatgac tgtgcgaagg tggcggaatt ggtagacgcg ctagcttcag gtgttagtgt
    52861 ccttacggac gtgggggttc aagtcccccc cctcgcacca attatcttta cttcctttct
    52921 tgtttcttcc ctgttttgtc atccctgatt caaattcatt atcatcagca tgatgccagc
    52981 aataagcgcc aggcacgatg gtagcaaaat gtaatgccgc aactgtttgt gatacagcat
    53041 caccggcgtc aatactaatc ccatcagaat gacaatccgc cactgatccg gcgataaatc
    53101 cgcatacatc agccctgcaa tcagcaatcc cggcagcaac actccccagc cactgcccag
    53161 cgtacgttgc aacatggttt catctctttc attgataatg aaaaccaata tcatatgata
    53221 atttttatca tttgcaagcc agagaaatcc cttgctatcg agtaaaccta tcgataggat
    53281 tagcaatcat tatcatttag attactatca cgattatggc ctatcgttcc gcaccgctct
    53341 atgaagatgt catctggcga acgcatctcc agccgcagga tgctgggctt gcacaagccg
    53401 tacgggcgat gatcgccaaa catcgtgaac atttgctgga gtttatccgc ctggatgaac
    53461 ccgctccgct taacgccatg acgctggcgc aatggtcatc accgaatgcg ctaagttctc
    53521 tactggcggt ctattccgat catatctatc gcaaccaacc aacgatgatc cgcgagaaca
    53581 aaccgctgat ctcactatgg gcacaatggt atatcggcct gatggtgcca ccattaatgc
    53641 tggcgctact gacgcaggaa aaagcattag atgtgtcgcc ggaacatttc catgctgagt
    53701 ttcacgaaac cggacgcgtc gcctgtttct gggtcgatgt gtgtgaagat aaaaacgcaa
    53761 caccacattc gccgcagcag cgaatggaaa cgttaatcag ccaggcgctg gttccggttg
    53821 tgcaagcact agaagcgacc ggagagatca acggcaaact tatctggagt aataccggtt
    53881 atttgatcaa ctggtatctc actgagatga agcaactgct cggcgaggct actgttgaat
    53941 cgctgcgcca tgccctcttt tttgagaaaa cgctcacgaa cggtgaagat aatccactct
    54001 ggcgtaccgt ggtactgcgc gacggcctgc tggtgcgccg cacttgctgc cagcgttatc
    54061 gcttaccgga tgtgcagcaa tgtggctatt gtacgctgaa atgaaagcac tgccggggaa
    54121 gtaaacccgg catcatgcgg attaataggg atgcaacaca ttacttgctt cgcaatgccg
    54181 catacataac agaatatctg cggcctccaa caaccctgca tcagaactga cgcccagttt
    54241 cgacatcaca ttaaatttat gcgctcggat cgttttgata ttacgtttaa gctgctcggc
    54301 aatttgtgtc attgagtagc cacgcgacat aaagcgcaat atttctctct ccgtcgggct
    54361 taacgtccgg ctttggttaa tgtaccactg attgttcagt cggtcggtcg cctgacgtac
    54421 accatttaat gacaaaaaga gttcctgatg aaaaatctcc agcgttgacg ctttacttaa
    54481 tacaccgtcc agcggtgatg gcgacaatga accaatcagc cgagcttcaa tatcatcatc
    54541 cacaataact aaacgccggg tacgcggaaa cttaatcgcc agctcagtca ggcaagataa
    54601 tccctctctg cgctcacttc tcatggcaga aaaagaaaaa atgaccgccg caaacgagat
    54661 ccgcgacatg gcatcctgaa atgactcctg actggtatag gtatggagct tataagggtt
    54721 gcccgcaaac tttctgaata aactctcaat accgatacta ctcatgacgc atttttctat
    54781 gagtgcgaca tttctcttct taattcggct gtgttccatt ctgcggcatc tccataaaca
    54841 gaaggcaagt taagttctct tgcaccctgc gcgctattta tccatgcata catttcagcg
    54901 ttgctgtgaa ttgccagtcg acgcatcgca ctgttttttt gggcgctgat agttttatta
    54961 cttttcttaa gcaatgaagc tatctggttg atgccccatc ctttacctaa tagccgcagt
    55021 accttgcgct ctgaaagagt gagaatgacg gagcgatagc taaaatcatg agtttcagtc
    55081 attgccggag aggtcagcat ggcgctgata cgctctgcat gcgtattgcc ggaacgtacg
    55141 gtcttgacca gattctcaat gggttcaaca tcagacaata acgtcgccgt agggcacatg
    55201 agcagttcta cagcctggga ataccaggag cgtgacacca tgaaaaccca gtgaatttcc
    55261 cgatattgtg agattaaaga ataataatgt tcacaaacag aacgtggatc ttcactttca
    55321 ccggctaaat cagcaatgac ta
//
LOCUS       DAETPZ010000020        54205 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.020, whole genome shotgun sequence.
ACCESSION   DAETPZ010000020 DAETPZ010000000
VERSION     DAETPZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.020"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..520
                     /locus_tag="K0W59_001324"
                     /pseudo
     CDS             1..520
                     /locus_tag="K0W59_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
     gene            524..1336
                     /locus_tag="K0W59_001325"
     CDS             524..1336
                     /locus_tag="K0W59_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022434.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(1348..2952)
                     /locus_tag="K0W59_001326"
     CDS             complement(1348..2952)
                     /locus_tag="K0W59_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HBI3022435.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPCEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(3078..3383)
                     /locus_tag="K0W59_001327"
     CDS             complement(3078..3383)
                     /locus_tag="K0W59_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBI3022436.1"
                     /translation="MATLLQFHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(3491..3567)
                     /locus_tag="K0W59_001328"
     tRNA            complement(3491..3567)
                     /locus_tag="K0W59_001328"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3531..3533),aa:Val,seq:gac)
     gene            complement(3572..3648)
                     /locus_tag="K0W59_001329"
     tRNA            complement(3572..3648)
                     /locus_tag="K0W59_001329"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3612..3614),aa:Val,seq:gac)
     gene            3956..5212
                     /locus_tag="K0W59_001330"
     CDS             3956..5212
                     /locus_tag="K0W59_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HBI3022437.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYAYGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYCLGT
                     DGLQLYSSGKTQNLSVNVGDRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(5253..6626)
                     /gene="mdtK"
                     /locus_tag="K0W59_001331"
     CDS             complement(5253..6626)
                     /gene="mdtK"
                     /locus_tag="K0W59_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HBI3022438.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            6841..7482
                     /gene="ribE"
                     /locus_tag="K0W59_001332"
     CDS             6841..7482
                     /gene="ribE"
                     /locus_tag="K0W59_001332"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBI3022439.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(7522..8670)
                     /gene="cfa"
                     /locus_tag="K0W59_001333"
     CDS             complement(7522..8670)
                     /gene="cfa"
                     /locus_tag="K0W59_001333"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HBI3022440.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(8961..10172)
                     /locus_tag="K0W59_001334"
     CDS             complement(8961..10172)
                     /locus_tag="K0W59_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HBI3022441.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRTALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            10285..11217
                     /locus_tag="K0W59_001335"
     CDS             10285..11217
                     /locus_tag="K0W59_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3022442.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(11214..12239)
                     /gene="purR"
                     /locus_tag="K0W59_001336"
     CDS             complement(11214..12239)
                     /gene="purR"
                     /locus_tag="K0W59_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HBI3022443.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            12538..12627
                     /gene="ynhF"
                     /locus_tag="K0W59_001337"
     CDS             12538..12627
                     /gene="ynhF"
                     /locus_tag="K0W59_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HBI3022444.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            12793..13962
                     /locus_tag="K0W59_001338"
                     /pseudo
     CDS             12793..13962
                     /locus_tag="K0W59_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(14108..14689)
                     /gene="sodB"
                     /locus_tag="K0W59_001339"
     CDS             complement(14108..14689)
                     /gene="sodB"
                     /locus_tag="K0W59_001339"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HBI3022445.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(14817..15644)
                     /gene="mepH"
                     /locus_tag="K0W59_001340"
     CDS             complement(14817..15644)
                     /gene="mepH"
                     /locus_tag="K0W59_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HBI3022446.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATAHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            15979..16326
                     /gene="grxD"
                     /locus_tag="K0W59_001341"
     CDS             15979..16326
                     /gene="grxD"
                     /locus_tag="K0W59_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HBI3022447.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(16365..16865)
                     /gene="tnpA"
                     /locus_tag="K0W59_001342"
     CDS             complement(16365..16865)
                     /gene="tnpA"
                     /locus_tag="K0W59_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBI3022448.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            16873..18081
                     /locus_tag="K0W59_001343"
     CDS             16873..18081
                     /locus_tag="K0W59_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3022449.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNAAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            complement(18125..22742)
                     /locus_tag="K0W59_001344"
                     /pseudo
     CDS             complement(18125..22742)
                     /locus_tag="K0W59_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(22835..23482)
                     /gene="rnt"
                     /locus_tag="K0W59_001345"
     CDS             complement(22835..23482)
                     /gene="rnt"
                     /locus_tag="K0W59_001345"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HBI3022450.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(23585..23992)
                     /gene="gloA"
                     /locus_tag="K0W59_001346"
     CDS             complement(23585..23992)
                     /gene="gloA"
                     /locus_tag="K0W59_001346"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBI3022451.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(24073..25170)
                     /gene="nemA"
                     /locus_tag="K0W59_001347"
     CDS             complement(24073..25170)
                     /gene="nemA"
                     /locus_tag="K0W59_001347"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HBI3022452.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGRAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(25207..25806)
                     /gene="nemR"
                     /locus_tag="K0W59_001348"
     CDS             complement(25207..25806)
                     /gene="nemR"
                     /locus_tag="K0W59_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HBI3022453.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLIFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            25909..26148
                     /locus_tag="K0W59_001349"
     CDS             25909..26148
                     /locus_tag="K0W59_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HBI3022454.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            26197..27093
                     /locus_tag="K0W59_001350"
     CDS             26197..27093
                     /locus_tag="K0W59_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HBI3022455.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGMLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            27174..27695
                     /gene="sodC"
                     /locus_tag="K0W59_001351"
     CDS             27174..27695
                     /gene="sodC"
                     /locus_tag="K0W59_001351"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HBI3022456.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(27696..29678)
                     /locus_tag="K0W59_001352"
                     /pseudo
     CDS             complement(27696..29678)
                     /locus_tag="K0W59_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(29671..30462)
                     /locus_tag="K0W59_001353"
                     /pseudo
     CDS             complement(29671..30462)
                     /locus_tag="K0W59_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            complement(30465..30701)
                     /locus_tag="K0W59_001354"
     CDS             complement(30465..30701)
                     /locus_tag="K0W59_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HBI3022457.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            30902..31336
                     /gene="slyA"
                     /locus_tag="K0W59_001355"
     CDS             30902..31336
                     /gene="slyA"
                     /locus_tag="K0W59_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HBI3022458.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(31383..31850)
                     /gene="slyB"
                     /locus_tag="K0W59_001356"
     CDS             complement(31383..31850)
                     /gene="slyB"
                     /locus_tag="K0W59_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HBI3022459.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            32124..33233
                     /gene="anmK"
                     /locus_tag="K0W59_001357"
     CDS             32124..33233
                     /gene="anmK"
                     /locus_tag="K0W59_001357"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBI3022460.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            33331..33660
                     /gene="mliC"
                     /locus_tag="K0W59_001358"
     CDS             33331..33660
                     /gene="mliC"
                     /locus_tag="K0W59_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HBI3022461.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGFSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            33719..34375
                     /gene="pdxH"
                     /locus_tag="K0W59_001359"
     CDS             33719..34375
                     /gene="pdxH"
                     /locus_tag="K0W59_001359"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HBI3022462.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            34504..35778
                     /gene="tyrS"
                     /locus_tag="K0W59_001360"
     CDS             34504..35778
                     /gene="tyrS"
                     /locus_tag="K0W59_001360"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBI3022463.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            35837..36706
                     /gene="pdxY"
                     /locus_tag="K0W59_001361"
     CDS             35837..36706
                     /gene="pdxY"
                     /locus_tag="K0W59_001361"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HBI3022464.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAIAAIDKLHTCDAVLSGYLGSAEQGEHVLGIVRQV
                     KAANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAV
                     NNVEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGM
                     RQPVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDR
                     IAKPEHYFSATKL"
     gene            complement(36750..37355)
                     /gene="gstA"
                     /locus_tag="K0W59_001362"
     CDS             complement(36750..37355)
                     /gene="gstA"
                     /locus_tag="K0W59_001362"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HBI3022465.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(37461..38963)
                     /gene="dtpA"
                     /locus_tag="K0W59_001363"
     CDS             complement(37461..38963)
                     /gene="dtpA"
                     /locus_tag="K0W59_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HBI3022466.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(39574..40209)
                     /gene="nth"
                     /locus_tag="K0W59_001364"
     CDS             complement(39574..40209)
                     /gene="nth"
                     /locus_tag="K0W59_001364"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBI3022467.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKERVDI"
     gene            complement(40209..40904)
                     /gene="rsxE"
                     /locus_tag="K0W59_001365"
     CDS             complement(40209..40904)
                     /gene="rsxE"
                     /locus_tag="K0W59_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HBI3022468.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(40908..41528)
                     /gene="rsxG"
                     /locus_tag="K0W59_001366"
     CDS             complement(40908..41528)
                     /gene="rsxG"
                     /locus_tag="K0W59_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HBI3022469.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFGQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(41532..42590)
                     /gene="rsxD"
                     /locus_tag="K0W59_001367"
     CDS             complement(41532..42590)
                     /gene="rsxD"
                     /locus_tag="K0W59_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HBI3022470.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(42591..44717)
                     /gene="rsxC"
                     /locus_tag="K0W59_001368"
     CDS             complement(42591..44717)
                     /gene="rsxC"
                     /locus_tag="K0W59_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBI3022471.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVAAAIARVQAKKAAQQKVVNED"
     gene            complement(44710..45288)
                     /gene="rsxB"
                     /locus_tag="K0W59_001369"
     CDS             complement(44710..45288)
                     /gene="rsxB"
                     /locus_tag="K0W59_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HBI3022472.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(45288..45869)
                     /gene="rsxA"
                     /locus_tag="K0W59_001370"
     CDS             complement(45288..45869)
                     /gene="rsxA"
                     /locus_tag="K0W59_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBI3022473.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(45946..46386)
                     /locus_tag="K0W59_001371"
     CDS             complement(45946..46386)
                     /locus_tag="K0W59_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HBI3022474.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(46472..46687)
                     /gene="ydgT"
                     /locus_tag="K0W59_001372"
     CDS             complement(46472..46687)
                     /gene="ydgT"
                     /locus_tag="K0W59_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HBI3022475.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(46960..47085)
                     /gene="blr"
                     /locus_tag="K0W59_001373"
     CDS             complement(46960..47085)
                     /gene="blr"
                     /locus_tag="K0W59_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HBI3022476.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            47328..48368
                     /locus_tag="K0W59_001374"
     CDS             47328..48368
                     /locus_tag="K0W59_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBI3022477.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(48404..49405)
                     /gene="add"
                     /locus_tag="K0W59_001375"
     CDS             complement(48404..49405)
                     /gene="add"
                     /locus_tag="K0W59_001375"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBI3022478.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(49508..50680)
                     /gene="malY"
                     /locus_tag="K0W59_001376"
     CDS             complement(49508..50680)
                     /gene="malY"
                     /locus_tag="K0W59_001376"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HBI3022479.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(50690..52282)
                     /gene="malX"
                     /locus_tag="K0W59_001377"
     CDS             complement(50690..52282)
                     /gene="malX"
                     /locus_tag="K0W59_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HBI3022480.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            52457..53485
                     /gene="malI"
                     /locus_tag="K0W59_001378"
     CDS             52457..53485
                     /gene="malI"
                     /locus_tag="K0W59_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HBI3022481.1"
                     /translation="MAAAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            53597..>54205
                     /gene="hdhA"
                     /locus_tag="K0W59_001379"
     CDS             53597..>54205
                     /gene="hdhA"
                     /locus_tag="K0W59_001379"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HBI3022482.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFAWRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITP"
BASE COUNT        13680 a        13578 c        14078 g        12869 t
ORIGIN      
        1 aagtgggctg ataaatcact ttgcgatggt gggcgcaact gcggttaaaa gtctggttgc
       61 ggtgcatgaa gtttgctgat ttttgttact ggcatgctgg ctcggctttg tgctgatcaa
      121 tgccgttggg gataatggtc accactatcg cattcgtcgt caggggtggc tggaacgagc
      181 ggcaaaacaa acgcgatttt atttgtttgg cattatgcag ggggaagaac atcctttccc
      241 ggcaacaaca cagtctaaat ttaatccctt acagcaggtc gcctatgttg gtgtcatgta
      301 tggattgctg ccgttgttac tattgacggg gctgttgtgt ctctatccgc aagccgtggg
      361 agatgtgttt cctggcgtaa gatactggtt attgcaggcg cattttgctc tggcatttat
      421 aagcctcttt tttatcttcg gtcatcttta tctttgcacc acggggcgta cgccacacga
      481 aacctttaaa agcatggtcg atggctatca ccggcactaa cgaatgatca ttacacgagc
      541 agacctgcga gaatggcgta tcggagcggt gatgtatcgc tggttcctgc gccatttccc
      601 acgcggcggt agttatgccg atattcgtca tgcactgatt gaggaaggat atacggactg
      661 ggcggagagt ctggttgagt atgcgtggaa aaaatggctg gcggatgaaa acttcgcgca
      721 tcaggaagtt tcctctatgc aaaaactggc cacagacccc ggagagatac cattttgcag
      781 tcagtttgcc cgcagcgacg atcatgctcg catcggctgt tgtgaggata acgcccgaat
      841 cgcaaccgct gggtatgccg cgcaaattgc cagtatgggt tatagcgtgc ggattggaag
      901 tgtcggcttt aacagtcaca ttggcagctc cggtgaacgc gcacgggttg ctgtaaccgg
      961 taattcttcg cggataagca gcgctggaga tagtagtcgg attgcgaata ccggaatgcg
     1021 agtacgcgtt tgtacattgg gtgaacggtg tcatgtcgcc agtaatggtg acctggtgca
     1081 aatcgccagc tttggcgcaa acgccagaat tgccaacagc ggcgacaatg tacacatcat
     1141 tgccagcggt gaaaattcaa cggtagtgag cacgggcgtg gtcgactcaa ttattctcgg
     1201 gctaggggga agcgccgcac tggcatatca tgacggcgaa agagttcgct tcgccgtcgc
     1261 cattgaaggt gaaaacaata ttcgaaccgg agtccgctat cgtcttaatg aacagcatca
     1321 gtttgttgag tgctgatagc tttaccttta atcaaacgaa agacgccgac gagcacggga
     1381 tgcaggcttt acgaccgccc gagccatcgc ctcaccaatt tctgcacatg ccgtaagtcc
     1441 ctgaacgaaa ggctggtcgt gcatcagcca gggcaacgcg ccgaacgcta cgcgtccacg
     1501 aatatcttcg ggttgctgta acgtataatc ttcgccaaca tcagggattt catcccctgt
     1561 tttctgtaat tgctcgcgta gcccagggaa aggaatatct ttcactttaa gcggacgctg
     1621 tccgcgggca tcaataaaaa cgtcaaacga gtagctgttg tcttccgttt tcaggacggt
     1681 gcgcgactca ttaatttcca tttcgtagtc ttcaccgagg gcgagaatat gaatgatccc
     1741 cgcttcacgt aaggccagta gtcgacgaat agactctgaa gggattgccg cataattatc
     1801 gatgaaaacc ctggcaaggc ctttactgaa ccgtttatgg tcgtgttcat tcagatgagg
     1861 aacaatttcc tgtacggctt catgcaggcg cagaatgaca taacgccagg gaacagtatg
     1921 tttttctcgt ttattgcgtt cgacttcctg gagatttttt tctgcccagt caaatgggtc
     1981 gcgttgtttg cgctcggcaa accaggcttg agcaaaggaa tcgacattca ggctctctaa
     2041 ggctatgcgt tggctccagt ctggatcagc aaacttgatt tcctctacta tcaatctaaa
     2101 tacccgatcc aaaaggccct cttcaccttt ttgaatctca gcatttaatg cctgatcggt
     2161 gacgatgtgt aagggctcgc agggaatagg gcaatagaaa tcggcttcgg gtaaaatacc
     2221 cgtgcgcgac atcaacgtaa tatttagctt ttcacttgcg ttatcgcggt gaaaaacgac
     2281 gtgttgttta tcatcttcaa tgaacgaacc atgctgaata gccactgcca ttgccgcatc
     2341 cagtccgctc aaggatgttc ccataatacc cacgttacac gcatcgacct ttgcttccat
     2401 caggcctgac cacgggctgg gaaaatacgt tctggttgct tcttcttcat caggccagac
     2461 gtgacccgtg gcaatcaccg ctaaatcaaa cgtctcgctg ggtaaatcct gatttgtagc
     2521 gagcatgacg ccagcatttg taatttgcag atcggtaacc tggcatgatt cataaacagc
     2581 cactgcaaat ttttgctgtc gtgcctggtc tactaatcgt aaaaattgat cgcggaaata
     2641 ttcgcccagc agaattcgcg gtaaaaactg acgatcgtgc aaggtttctt ttttaacgcc
     2701 ataacgctgg agatggctgg cttcttgctt ttgtagccat tcgagatacg tacaattaat
     2761 cggcggtatt tcaatactgg caatatttgc cagcatcatt tttgagtttt cctcatcact
     2821 gtatggcatc ccgacaccgg cctcgtcagc ctgctcgaag atagaaatag aaagtggagt
     2881 ttgttgctgt agaagcgaga ataaggtgta gatccccgta ggcccggcac ccacaatagc
     2941 aatttttttc attgttactt acctgtacgc attctttcga atttagtgtt atcagataag
     3001 tttggttgag atttttagaa tttgagaaat ttcagacagg gatgaaaagg gcgctaaccg
     3061 cccttgatta aattctgtca ggcgagtttt gcctgattga tttgactaag tggctcattg
     3121 acatcgaaaa ctttggcgac aacttcctca acgccgagat ttttcagtcg ggcagtatgt
     3181 ttttccagat aggcaagggc gcttttttca tcagtgaaaa ggtagatccc accggcttcg
     3241 tggttctttt cactttctgt ccataccttc cacagaaaac caggttcctg attaatcgac
     3301 tcagcaagtg gtttaagctg ctcagccatt gcgtcgccaa atgggccatt aaaagcaaaa
     3361 tgaaattgta acaaagttgc catagcgatt ctccttctga taacgcagac gaagacagtg
     3421 taaatctttt aacgtattgg atacagagaa gaaacagagg gaaaaaggaa aagaccagat
     3481 taagaaaatc tggtgcgtcc gagtggactc gaaccaccga cccccaccat gtcaaggtgg
     3541 tgctctaacc aactgagcta cggacgcagg atggtgcgtt caattggact cgaaccaacg
     3601 acccccacca tgtcaaggtg gtgctctaac caactgagct atgaacgcaa cgttgtaggt
     3661 gacaacgggg acgaatatta gcggcagagt ggaaaggtgg caagaggcaa aacgtaattt
     3721 tctgcgctat ttcgaccgtt tgcagagctt ttaagcaaat tggctatatt ttgttgattt
     3781 gcaagggcga tttttattca ggatcgcatt tacatctgat acaacccaat aattcgtgta
     3841 tcagagttta aacagagttg tttgccacca tctcaaatct gtcgtggata aatcctccgt
     3901 agcctttaca cttaggtagc aataaaggca cacagatgca gcaaaggaat aaatcatggg
     3961 atctgatgcg aaaaacttga tgagcgacgg gaatgtgcaa attgttaaga ccggcgaggt
     4021 cattggcgcg acgcaactta ctgaaggcga gttgattgtt gaagctggcg gaagagccga
     4081 aaataccgtg gtcacggggg ctggctggtt gaaagtggca accggtggga tcgccaaatg
     4141 cacacagtac ggtaacaatg gcacgctatc ggtcagcgac ggtgccattg ccacagatat
     4201 tgttcagtcc gagggaggcg caattagtct ctctacgctc gctacggtta atggccgcca
     4261 tcccgaaggt gaattcagcg ttgatcaggg ttatgcctac ggtttgttgc tggaaaatgg
     4321 cggtaacctg cgtgtactgg aaggacatcg cgcggaaaaa atcattctcg atcaagaggg
     4381 cggcctgttg gttaatggga caacctcagc ggtcgtggta gatgaaggtg gtgaattgtt
     4441 ggtgtatcca agtggagaag ccagcaattg tgagattaat cagggcggcg tttttatgct
     4501 ggccgggaaa gccagtgata cgttgcttgc tggtggcacc atgaataatc tcggtggtga
     4561 agactctgac actattgttg agaatggatc catttattgt ctggggacgg atggccttca
     4621 gctctacagt tccggtaaga cgcaaaacct gtcggtgaat gtgggggatc gggctgaagt
     4681 gcatgccggt acgctggaaa atgcggtaat acaaggtgga acagtgatcc tgttgtcacc
     4741 caccagcgcg gacgaaaatt ttgtcgtaga ggaagatcgc gcaccggttg aactgaccgg
     4801 gagtgttgca ttactggacg gcgcttcaat gattattggt tatggcgcag agctgcaaca
     4861 atcaacgatt actgtacagc agggcggtgt gttgattctc gacggcagta cggtaaaagg
     4921 tgacagcgtc actttcattg ttggtaacat caatctgaat ggcggaaaac tgtggctgat
     4981 cactggtgcg gcaacgcatg tgcaactgaa agtgaaacgc ctgcgcggag agggagcgat
     5041 ttgcctgcaa accagtgcga aagaaatttc acctgacttc atcaatgtga aaggggaagt
     5101 taacggggat atacgcgttg agataacaga tgccagtcgg caaactctgt gtaacgctct
     5161 gaaattacag ccagacgaag acgggattgg cgcaacgctc cagcctgcgt aaaaacacag
     5221 gcgcaacccg tgaaggttgc gccttgtctt tattagcggg acgctcgttg cagaatgatg
     5281 gctgacggca tacgttgcag gaaccgcata cgcagcatca tcataatggc tgccgatgtc
     5341 aggccgataa taaagcctat ccagaagcct gctggcccca taggttcaac gaccagatcg
     5401 gtcagtgcca gaatatagcc gcttggcaag cccagcaccc agtaagctgt aaaggtgata
     5461 tagaaaatgg aacgcgtatc tttataacca cgcaaaatcc cactgccaat cacctggatt
     5521 gagtcagaaa tctgatatac cgccgccagc aacatcaaat gcgcagccag cgttacaacc
     5581 tcgggattgt cgttgtacaa cagggcgatt tgctcccgca gtgaaaccgt gaaaatggcc
     5641 gtcagggttg ccatacagac acccaccata agcccggtcc gcgcagcggt ttgcgcatcc
     5701 agcgttgagc cctgacccag acgataacct acgcggatag ttaccgctgc cgccagcgac
     5761 attggaagca caaacatcag tgaactaaag ttcagggcaa tctggtgtcc tgcgacatca
     5821 acaataccga gcggagacac taacagagcc acgacggcaa acagtgtcac ttcaaagaac
     5881 agtgccagcg caatcggcaa accgagttga atcagtcgtt tcataaccgc aggatcgggc
     5941 ttagcggtgc ctttttcgtt acgaatatcg cgcatggagc gggcgcgttt aatgtaagaa
     6001 accatggcaa ggaacatgac ccaatacacc gccgcagtag ccacgccaca accaacgcca
     6061 ccgagctcag gcataccgaa atgaccataa ataaagatat agttcaccgg gatgttcacc
     6121 agcaggccga taaagcccat taccataccc ggcttggttt ttgccagacc ttcacattgg
     6181 ttacgggcaa cctggaagaa cagatatccc ggcgcgcccc acaacaacgc acgcagataa
     6241 cccacggctt tgtccgccag agccggatcg atgttttcca tggagcggat aatgtaacct
     6301 gcattccaca gcaccagcat aatgagaacg gaaacaaaac ctgccagcca gaaaccttgt
     6361 cgcacctgat gcgcaatgcg ctcacgtcga ccggaaccat ttaattgcgc gataaccggc
     6421 gttaatgcca gcagcagtcc gtgaccaaag aggatcgccg gaagccagat agaagtaccg
     6481 atagcgaccg ccgccatgtc ggtggcacta tagccgcccg ccatcacggt atcgacaaaa
     6541 cccatcgcag tttgggcgat ttgcgcgaga atcaccggga ttgctaatgc taataacaga
     6601 cgcgcttcac tgatatactt ctgcacgtga acacctttta tttgtagtta tatgagagac
     6661 taaaaagccg cctgaatggg cagcaaaaag aagaagcagg ggaaattcag tctattgtag
     6721 cgaggattta ttaattctcc agtgaaaaaa tagccattct taccccttca ctggtaaggg
     6781 cgttttccac ctgctattgt gctgaacaga atgtcttaac tgatttcagg agttgtaagt
     6841 atgtttacgg ggattgtaca gggcaccgca aaactggtgt cgattgacga gaaaccaaat
     6901 tttcgtacgc atgtggtgga gttacccgac cacatgctgg acggcctgga aaccggtgct
     6961 tccgtggcgc ataacggttg ctgcctgacc gtgacggaaa ttaacggcaa ccatgtcagt
     7021 tttgacctga tgaaagaaac gttacgcatt accaatcttg gcgatttaaa agtgggggat
     7081 tgggtaaacg ttgagcgtgc ggcgaaattc agtgatgaaa ttggcggaca cttaatgtca
     7141 ggtcatatta tgaccaccgc tgaagtggcg aaaatattaa cctcggaaaa taatcgccag
     7201 atctggttta aagtccagga tagtcagttg atgaaatata tcctgtacaa aggatttatt
     7261 ggcatcgacg gtattagcct gaccgtcggc gaagtcacgc caacgcgttt ttgcgtccat
     7321 ttaattccgg aaacactgga acgcacgact cttgggaaga aaaaacttgg cgcacgcgtc
     7381 aacattgaaa tcgatccaca aactcaggca gtggtagata cggtagaacg tgtgctggcg
     7441 gcacgagaaa atgccatgaa tcaaccaggt acagaagcct gatctgaact cgcccccgaa
     7501 gagggggcga tagaatagcc tttagcgagc gactcgaagg ccgttttcaa caccgcgtga
     7561 gaacaccacc tgccagagct gaatatcacg ggcgcggaaa gcacctgcgc aggcattcag
     7621 ataataggta aacatccgtt taaagcgttc actgtagtta tccgcaattt ctggccatgc
     7681 ggcgaggaat cgttcatacc acgccatcaa cgtagtatcg taatcagcac cgaagttatg
     7741 ccagtcttcc atcacaaagt ggggttcgct ggattgagca atctggcgta cagagggcag
     7801 gcaaccgttc ggaaaaatat atttattaat ccagggatca acattcagat cggttttttt
     7861 cgaaccgata gtatggagca ggaatatacc ttccggtttc aaattacgat ccaccaccgc
     7921 aaaataggta tcgtaatttt tcggtccgac gtgctcgaac atccccacag aaacaatacg
     7981 atcaaactgg tcgttcaggt cacgataatc ttgcagcaaa atggtgacat ccaggccttt
     8041 acagcgttcc tgagccattt tttgctgttc ggcagaaatg gtgacgccga ccacgcttac
     8101 gtcatagtta gatgccatgt agtgcgccag accgccccag ccgcagccaa tatccagtac
     8161 gcgcatccct ggttttaact gcaatttttc acaaatcatt ttgagcttcg cctgctgggc
     8221 agattccaga ttatcggcat ctttccagta agcgcaggaa tattgcatga agggatcaag
     8281 catgcggctg aacaagtcat tacccaaatc gtaatgctcc ttgccgacta tccaggcacg
     8341 ttttttactc tgcagattga agagacgagc gccggcaata cgcagcgtgt ctttgaaatg
     8401 atgggggagt tggttttcga gacctgcgcg taagactttg ctaaaaaaca tatccagtcg
     8461 gtcacattcc caccagccat ccatataact ttcgcctaac cccaaagagc cttcctgcag
     8521 aacgcgttta aaaaaatcgg ggtttttcac acgaatatcc gccggggcag aaccgttaat
     8581 ggctataccg gcacggctaa gtaattcgtt ggcgatacgg taccagttgt catccggtac
     8641 actgacttct tctatacacg atgaactcat agtttctcca tcactggtcg gtgatcagaa
     8701 ccttaaaaca gcgtagacgc tttttgggct ttgtgagaaa tctcgcagaa aaaaccgcga
     8761 gtctataatc cgacgtagaa cagagggaaa ggaggaaccc ttgccgaaat gaccatccat
     8821 agtgagtcgg gaacgatcct gtccccgtaa aaacataata tttatcgtag ctttaacaac
     8881 tcgaaattag tataggccgc taatttcaat catacaacaa aattttgtta gcagcctaag
     8941 tataagtata tcgatataga tcagtgtgat tcgctatgag cgacttcggc attgccatga
     9001 ttcgggcagt caacttcttc acaacgttgc atcatgtaac ccagcgcgac cagcactact
     9061 gttgataaca tcacgctggt ggtggtgagc aatggcgtgc tgatactaat aagccaggaa
     9121 actaccagac ttgcgaggaa gcacagaccc agttgcagag tgttctgcaa cgctgcggcg
     9181 cgaccggttg catgtgggaa gggacgcagc gcctgggcga caacaatagg gtagatcgcg
     9241 ccattggcaa tcgccatcac acagaatggg atcaggattt cgaccagcga cacatggcta
     9301 atgaagcccg cagcccaggt cgcaatgacg ctgacagcaa acagcaccag caaccacggt
     9361 aataactgct tgccttgcca tttctgcagt gcggtgcgac agccataacc accaatcaga
     9421 aacgcgatag tttgcgggac ataacttaaa ccaataactg ccgggctgta gcccatttca
     9481 ctaaggatga acggtgaacc ggtcagccat gcaaaaaaac tggctgaaca ggctgcgtat
     9541 atcagcacgt tgccgcgata ggttttagaa cgtagcaggt cggtaaaggt cagaccatcc
     9601 tgactattgt tacgggcctt cgtcgtgggt ttgagccaga aaataggcag aatcagcacc
     9661 acggtaatgg caaacagggt ggcgaaaatc gcctgccagg aaaaatggac cagcagccag
     9721 cttcctaaca ggggagccag tgccggagac agacccacca gcggcatgat ggtcgcaaaa
     9781 atacggttga ctttctgtga aggataataa tctgtcacta atgcttgcca gataaccgcc
     9841 gcggcgcaga cccccacagc ctgtacaaaa cgcaatacca gcagcgtggc ggcgttttct
     9901 acccacagca tccccagact acctaacgca aaaattgtca ggccgattaa taacaccggt
     9961 ttacgaccat aacggtcgga gagtggcccc cacagaagct gggctgcggc aaaaccggca
    10021 aggaacagac taaggctggc actgacagca gacgcaggcg tttgcaggtc ggcctgtatg
    10081 gcggcgaaag caggcagata catatcggtt gccagaaaac cgagtacgct caaacccgcc
    10141 agccagacta aaaatctttt cccaggttgc atgtatattc tctaaatctc tcaattaaac
    10201 accgccgcaa agtgtaggga gtgaaattag tgttgtgaaa cggtaatatt tgatgactgg
    10261 tttcaaaaat tttgcaggct gactatgtgg tcagaatatt cactcgaagt tgttgatgcg
    10321 gtagcgcgta atggtagttt tagcgctgcg gcacaggagc tgcatcgcgt tccttctgcg
    10381 gtcagctata ccgtgcgtca gctggaagag tggctggcgg taccgctctt tgaacgacgg
    10441 caccgtgatg tggagctgac cgctgctgga gcgtggtttc tcaaagaagg gcgctctgtt
    10501 gtcaaaaaaa tgcagatcac ccgccagcaa tgtcagcaga tagcgaacgg ctggcgcggt
    10561 cagttagcta tcgcagtgga taatattgtc aggccagaac gtacacggca gatgatcgtt
    10621 gatttttatc gccattttga tgatgttgaa cttcttgtct ttcaggaagt gttcaacggt
    10681 gtctgggatg cgctttccga cgggcgcgtg gaactggcaa ttggcgcaac acgcgcgatt
    10741 ccggtaggcg gtcgttatgc cttccgggat atggggatgc taagctggag ttgcgttgtt
    10801 gccagtcacc acccgctggc gttgatggat ggcccgttca gcgatgatac gttgcgcaac
    10861 tggccgtcgt tggtgcgcga agacacctcg cgaacgctac ctaaacgtat tacctggttg
    10921 ctggataacc aaaaaagact tgtcgtacct gactgggaat catcggcaac ctgtatctcg
    10981 gcaggattat gcatagggat ggtgccaaca catttcgcca aaccgtggct taatgagggg
    11041 aagtgggtag cacttgaact ggaaaacccc ttcccggatt cggcatgttg tctgacatgg
    11101 cagcaaaatg atatgtcgcc tgcgttaacc tggctgcttg agtatctcgg cgatagtgag
    11161 acgctgaata aggagtggct gcgggagccg gaagagactc ccgcaacggg tgattaacga
    11221 cgatagtcgc ggaacgggcc gtcagccacg gagcggcgtt caatcaaacg cggatgcact
    11281 tcaatggact gcggttcttc acgtttgttg acgatacgat ccaacagcat gttgaacgct
    11341 gtttcaccca gcgaatcttt tggctgatgg atcgtggtca gcgccggcgt aaaatagcgc
    11401 gcgttgcgca cgttatcata accgatcagc gaaacatcct gcgggacgcg caggcccatt
    11461 tcatcagcag cacaaagtgc gcccattgcc atgatatcgc caccacagaa gacggcagta
    11521 gggcgatgcg gctgcgacag gatttgctgc atggcgcgat aaccggattc aggttcaaag
    11581 tcaccctgca caatccagct ttccggcacc ttgatcatcg cttcttccat cgccttcata
    11641 aaaccggcaa ggcggcctgc gccggtgtta cgttccagcg ggccggggat gacgccgatt
    11701 tcgcggtgac cgcgttcaat cagataacgc ccggccatgt agccgccttc gaacgcgtta
    11761 tcaatgaccg catcggtgaa gtcagctttt gcttcacccc agtccatcac caccattggg
    11821 atatggcgat actcttccag catcgccagc aacggctctg ggtactcaga acacatcacc
    11881 agcagaccat cgacgcgttt ttgcgccatc atcgacagat aagcccgctg tttctcaaga
    11941 ttgttccacg cattacccag aatcagggtg taacctttct ggaagcaatt tttttcaact
    12001 gcttcaatga tctcggcaaa ataggccgct tcgctgctgg tcgccagcaa accgatagac
    12061 ttggtgtggt taaccttcag gctacgcgcc accgcgctag gggagtagtg taattcttta
    12121 atcgctgccc acacggcgtt gcgcgtttct tcagcgacga aacgtgtttt gttgatcacg
    12181 tgtgacacag ttgtagtgga aacgtttgct cgtttcgcta catcttttat tgttgccatt
    12241 ccatttcact ccagacccta acttcagctc ctgcaaaatc gcaaggtaaa cgtttgcctt
    12301 tacacacctt acgcacaaca aaagtgttgc ggtacgccgg aaaaacgaca cggagcgtca
    12361 ggaaggggtc aatggccagt acgcaaatag tgtaagcgtg gaattttggc cgatcttgac
    12421 gaaaagggga agtggtaaaa acacttatca ttataaatga cgggagattt tttcatcaca
    12481 gtgtgtaaaa atgcgtaata tcattttttg tgtggtaata aaaaaggaga aaacttgatg
    12541 agcaccgacc ttaaattttc actggtaaca acgattatcg tcctcggttt gatcgtagcc
    12601 gtgggtttga ctgccgcgct gcactgattt cctctcgagg gagcgcgttc tccctcgcat
    12661 ctccttcctg tttgttagca aaatggcaaa agtgttttct ctttttgtta ttttcatttt
    12721 tttgtgattg ctgtcatgct ttcgccactt taatgaatat cgcgtaatga cgcgaagagg
    12781 tggagataag gcatgaaaat taactatccg ttgctggcgc tggcgattgg cgcgtttggt
    12841 atcgggacaa cggagttctc gccaatgggc ttgttgcccg tcattgcgcg cggtgtggat
    12901 gtctcgattc ccgctgccgg aatgttaatc agtgcctatg cagttggcgt aatggtgggc
    12961 gcgccgctga tgacgcttct actttctcat cgtgcccgcc gcagtgcgtt gattttcctg
    13021 atggcaattt tcacgctagg caacgttctt tccgccatcg cgccggatta tatgaccctg
    13081 atgctttcac gtattttgac cagcctgaat cactgagcat tttttggttt gggttcagtc
    13141 gtggccgcaa gcgtggtgcc aaaacataaa caggccagcg cagttgccac tatgtttatg
    13201 gggttaaccc tggcaaatat cggtggcgtg ccggcggcga ccaggttggg tgaaaccatc
    13261 ggctggcgga tgtcatttct ggcaacggcg gggctgggag tgatttcaat ggtaagtctg
    13321 ttcttctcat tacctaaagg tggtacaggg gcacgacctg aagtgaaaaa agagctggcg
    13381 gtattaatgc gtccgcaggt gctgtctgca ttgctgacga cggtactggg agctggtgca
    13441 atgtttaccc tctacaccta tatctctccg gtactgcaaa gtattaccca cgcaacaccg
    13501 gtgttcgtca cggcaatgct ggtgctgatt ggtgtcggat tctctatcgg taactatctc
    13561 ggcggcaaac tggcagatcg ttcagttaac ggcacgttga aaggcttttt gttgctgctg
    13621 atggtgatta tgctggcaat cccgttcctg gcccgcaatg agttcggcgc agctattagc
    13681 atggtggtgt ggggcgcagc aacctttgcg gttgtacctc cgttacagat gcgcgtgatg
    13741 catgtcgcca gtgaagcgcc aggtctgtct tcatcagtca atattggtgc ctttaatctt
    13801 ggaaatgcgc tgggagcagc tgctggtggt gcggtaattt ccgctgggct gggatacagc
    13861 tttgtgccgg tgacgggggc gattgtcgcg ggactggcat tattgctggt gtttatgtca
    13921 gccagaaaac aacctgaaac agtttgcgtt gctaacagct aattaaacaa aaacgcagaa
    13981 ggataacctt ctgcgttaca gttgctgaca acgtgcgatt tatcttgccg aatgcgctgt
    14041 taacgcctca tccgcgttgg catggtaagc gaagcgcatc aggcaatgct gcatttgcca
    14101 tcagttatta tgcagcgaga tttttcgcta cgaattccca gttcaccagc gcccagaagt
    14161 gctccagata gccaggacgt gcattgcgat agtcgatgta ataagcgtgt tcccagacat
    14221 caacggtcag cagcggagtc gcatcggtgg tcagcggagt acccgcgtta gaggttgaaa
    14281 cgatagccag tttgccatcg ctgtttttca ccagccaggt ccagccagaa ccaaagtttt
    14341 tgatcgctgc atcagtaaac tgcgctttga aatcggcaaa gctgccaaaa gatgcggcga
    14401 tagcttcagc gacttttcca gtcggttcgc caccggcgtt cggtgccagg cagttccagt
    14461 agaaagtatg gttccagacc tgagctgcgt tgttgaatac gccaccttca gagctgcgaa
    14521 taatctcttc cagtgattta ccttcaaacg cggtaccttt aatcaggttg ttcaggttag
    14581 tgacataagt ctgatgatgc ttgccgtagt gatactcgat agtttccgca gaaatgtgcg
    14641 gtgccagagc atctttagca tatggtagtg caggtaattc gaatgacatt gctactctcc
    14701 tttattatta atttgcatac gtacaatagc cttattgtgc gtatggatag ggtagcaaat
    14761 taaaaggtga gacaaaagat gaacttaccc tgttgccgta acaacagggt aaagttttag
    14821 cgaagtgttt ttggggtcat tacccgacga gcgccaacat agtggcgctg ccagtagtct
    14881 tcactgagag aagtgatttg aatttcctga cctgtgcgcg gtgactgaat aaatttgccg
    14941 ttgccgacat acacgccgac atgatcggct gtaccgcgtc cctgagtacg gaaaaagacc
    15001 aggtcgccgt ttttcagctc actacgttcg ataggtcctg catcacgcag gtgatacatt
    15061 tcattcgccg tacgcggaat acgaattttc accaaatctt tataagcgta ataaaccagg
    15121 ccgctgcaat caaaaccggt acgcggtgag ctgccgcccc aacgatatgg cttaccaatt
    15181 tgctgcatca gtttattcat tgccactttc gtggcttttt gcaccttcgc tttgtgcgcg
    15241 tcggcaaggg ttcctgacgc ggcatttttg actttcacac aatgcgattt atagccctta
    15301 cgtttggtac acttttcggt aaagctggct gaggccgttt tattgacggc gtgtttgctg
    15361 cgagttttgg cgtttttaga agcggtaatg gaagattttt taacggaaga agcggttttg
    15421 cttttggtcg tacttttact ggcggctttt ttcgctgttt tctggctggc tttctgggtc
    15481 tttttggtgg ttgccgtgct tttcttttta tctgcttttt tggtgatatg agcggtagca
    15541 gaactctccc tggcttgctt tgaagcatgg gccataggcg ttaaaggcag ggtggtaaaa
    15601 agtaaagcac agagcgtgat cgaaatacgg tttatccgcg ccactgagcg tccctctgtt
    15661 aaggcaggtc tatcatgcct gcgtctgatt tacaaaactt gatgattcta atgcatagaa
    15721 gaagagaaag ttaatagctt ttattgttaa aaaatacgtt tcgttggcga gcgcaaaaaa
    15781 atgcagcagt gaaagctaaa aatggttata aatacgccag tttgccgcgc atcagagaag
    15841 aaacgaatat ttgaaatgca acaaatagag caaagggata aaatagcaaa agcgtgatga
    15901 aaatgttatt ttccgatgcc gcactgaccc gctacaatgt cagattactg ccgtaaagaa
    15961 gtttgaggaa gcaagacaat gagcaccact atcgaaaaaa tccaacgcca gattgctgaa
    16021 aacccgatcc tgctgtacat gaaaggttca ccgaaactgc cgagctgcgg tttctctgcc
    16081 caggcagtcc aggcgcttgc cgcatgtggc gaacgttttg cctatgttga tattctgcag
    16141 aatccggaca ttcgtgcgga actgccgaaa tatgctaact ggccgacctt cccgcaactg
    16201 tgggttgacg gcgagctggt cggcggttgt gatatcgtga tcgaaatgta tcagcgtggc
    16261 gaactgcagc agctgatcaa agaaactgcc gctaaataca agtctgaaga gccggacgcg
    16321 gaataatccg tcctgcaata ctgacggatg cgaacgggca tccgtcagtc ctttatccgg
    16381 tgcgccgtaa aacctcgtcc ttcagggcgg ggatataagg cgcggttttc cacctgacca
    16441 ggtgtttgct gttgttcaat gtattggcgg atgacggata ttggcgcacc ttcgcagcta
    16501 ctggcaaaat agccaggact ccacaaaacg cctttgtagt aatacctgac tgcaatatct
    16561 ggtcgatctc gtcgcagtaa cctattcgat atgcctttga ggctgtttac cagactggat
    16621 atcgccagtt tgggaggata gttaattaac aaatggacgt gatctggttc gccatccatt
    16681 tcaaccagtt cagctgcaaa atcagcacat acatttgaaa agtaagtgcg tagtttttct
    16741 gtcgtgtcat ggtcaaaaat ctggcgtcgg tatctggtga caaagaccag gtgaacatgc
    16801 atcaggaaaa cacaatgcct tccacgccgg atatcggttt cttttttcac agaccaaagt
    16861 atcatttgac ctgtgaaacg attacaggca tttaaattcc agttaagacc cggtggtcaa
    16921 caggagcgtg aaatgaggcg cttcgcaggc gcatgtcgtt tcgttttcaa tcgtgctctg
    16981 gcgcttcaga atgagaatca tgaggccggt aataaataca tcccttacgg gaaaatggct
    17041 tcctggctgg ttgagtggaa aaacgccact gaaacgcaat ggcttaaaga ttcgccctca
    17101 cagccattgc aacagtcact gaaagacctt gagcgggctt acaaaaactt cttccggaag
    17161 cgggctgctt ttccccgatt caaaaagcgg ggacagaatg ctgcattccg ctacccgcag
    17221 ggcgttaagc tcgatcagga aaacagccgt atttttctgc cgaaacttgg ctggatgcgc
    17281 taccggaaca gccgtcaggt cacgggagtt gtgaaaaatg tcactgtcag ccagtcctgc
    17341 ggtacgtggt acatcagtat tcagacagaa agtgaagtat caactccggt tcacccttca
    17401 gcatcaatga tcgggctgga tgctggcgtg gctaaactcg ccacgctgtc agatggcaca
    17461 gtctttgagc ctgtaaacag ttttcagaaa aaccagaaga cgctggcgag acttcagcga
    17521 cagttaagcc ggaaggtcaa attcagcaac aactggcaga agcagaaacg caaaatacag
    17581 cgactgcatt cctgtatcgc aaatatccgc agggactacc ttcacaaagt cacaacgacc
    17641 gtcagcaaaa accacgcaat gatagtcatt gaggatttga aggtcagcaa catgtcaaag
    17701 tcagcagcgg gtacggtcag ccagccgggg cgcaatgtcc gggcaaaatc aggtttaaac
    17761 cgttcgatac tggatcaggg ctggtatgaa atgcgccgcc agcttgagta caagcagctc
    17821 tggagtggcg gtcaggtgct tgctgttccg ccagcgtaca caagccagcg ttgcgcgtgc
    17881 tgtggtcata cagcgaaaga aaaccgcctg tcacaaagta aattcagatg ccaggtatgt
    17941 ggatatacag cgaacgccga tgtaaatggc gctcgtaaca ttttagcggc ggggcacgcc
    18001 gttcttgcct gtggagagat ggtgcagtca ggccgctcgt tgaagcagga acccaccgaa
    18061 atgattcagg cgacagcttg aacgtagcag ggaaccacgt ctttcagggc gtggaggatg
    18121 tcaactatcc ccaatccagc ccttgtggcg aacttgaaaa tcccgcctca cgcagcagcg
    18181 taaacatcgg cgtttgccgg accggaagat cattcacttc tgttagcgta aagcgcagcc
    18241 ttggttcgcg acgcagtgcg gtggtcagcg cctggaaaat ctctggggcg agtaattcct
    18301 ccttttcctg ccagaccagc atttttttgc cgccttgcgc cagatagagt aacaatttgc
    18361 cgccagaaac gaccaccagc gcacccgccc gacgcgttgg taccagcgct gccggatgtg
    18421 caggccaggg cagaagattt ccccacacat tagccggatc gttggcggag agcgccactg
    18481 gtgtatagtg gcgtgtttgc gtggcttgtg tcgccagatc gcgcaatcgg tcaatagtca
    18541 gacgttcagc gaattgcgcg ccacccagac cttctacaaa acgacctcgc ataattcgcc
    18601 cggagtcttc catacttcga caaagtgttt gcatcgatgg aaacccgcca gggatatttt
    18661 cggctatcac cgcctgacga ctgatgatgc cgtagcggtc gagcatattt tccgccagcg
    18721 ccagcatcct ttcggtatcg tttagtggct ccacctgcaa taacgaccag cgtccagcca
    18781 gatttggtgt gttgtaagat acccgcggcg agacgggttg tgcatagaca ggacgcccac
    18841 gacgagcccg gtgactgcgg cgagttgagg tgcgtgcgtt ggaactgctg cgggtgaggg
    18901 cgcgtaacgg tgcccaaata tcgctggtaa tgacgccttg ccagaccagc gcccataacg
    18961 cctcttgcag tgccgaggaa tcaaccgatt cgccgatttt atcgcgtatc cgttggctga
    19021 tttgttgtgc aaaccaggct cctccgtcag ccagaacagc gattatcgct tgctgcagcg
    19081 ccgaacgatt cgcctgatcc gcttcggcgg gagtgaacga ttctgcggca tatttctgta
    19141 gatgcagtgc caccaggccg tcatcttcac ccagcttttt tttgtcccga ccagataacc
    19201 gctccggttg ccagtaattc atcgagcatt tctggcgaat agtcgcgtac gcgggcaggc
    19261 aggatctggc tttcccagag tgaggcgggt aaaccgactc cggcaagctg ttcgatcacc
    19321 cgcatcacgc catctacgcc ctcataaacg cctggcgatg ttgaggcaaa gagcgccggg
    19381 ctaccatcgg tggcgggtaa tacgccctga cgttccagca gcaatcgcgc ataggtcgtg
    19441 gctgcaacgg gacgcgtcgc ttctctggcg gcttgcagcg agcgcaaacg cagacgacga
    19501 aatacttcat cgctcaccca gatgtcttgt tgcagattca tcaccagacc ctgttcacgc
    19561 agttgctgaa gctgctcttc gacaatggca ataccgagac taaactcatg agccagttgt
    19621 tcagccgtga ccagagcatg agcccggaga tagcgcagaa agaggtcgcg aagcgggtaa
    19681 ctgactctat gtaaataaat ctctggcaat gactctggta gtcgtacgcc gagggcatca
    19741 cgcagcctgg cggcatcatc catacaggca agacgctcct gtccgctaat catcgcaggg
    19801 aagattcgtt ttactgcaag aagattttcc agatacgacg caacctcttc actgctgcct
    19861 gtatgccgtt gcgccaggtc ttcaacggtc attggcccca gttcgcgcag caggtcgaac
    19921 aatccttctt cacctttcgc tcttctgcca ggagccagtc gttgcaactc ttcttccacc
    19981 tggcgaatga cctgcgggtc gagtaattcc cccggatcga cctgtccgag tagattgcgc
    20041 agtaactcgc tgtccagcga cagtacggat gcccggcgct ctgccagcgg ggcgtcgctc
    20101 tggtacataa attccgcgac atagccgaac aataaacttg tggcgaaagg cgagggcgta
    20161 gtggtcgtta catcggatat ttgaatttcg ccaccgttca ggcgacgcat caaacgttcc
    20221 agtgcgggaa gatcataaac atcttgcaga cattcgcgta gggtttcgag aatgaccgga
    20281 aaatccggat atccctgagc gatttccagc aactgactgg cgcgcagccg ttgttgccaa
    20341 agcggggtgc gatggcccgg cgtgcggccc ggcattaata atgcccgcgc ggcgcattcg
    20401 cgaaaacggg cggcgaaaag tgccgagctg cctaccgcct cgcggacaat ttgcagcaac
    20461 ttttctggtt caaacaaaaa aatcgcggcg tcgggcaatt taccatcagt gtcaggaatg
    20521 cgcgcaacaa tgccgtcatc actggcgacc acagacgcgt cagcgcccca tagggcatgt
    20581 attcgcccgg caatcgccag cgcccagggt tcatgcaccc gtcttccata gggagagtgc
    20641 aaaataatac gccagtcgcc aatttcatca cggcaccgtt cgaggaccag atggcgactc
    20701 cccggaacga tgcccgtcgc gttgcgttgc tcgtcaatca acccctgaat attggcgttc
    20761 gtattttctt ctgccagcca cgggggaata gtcccggaaa agaacgcgcc atccgccagt
    20821 aaatgaagaa aatcgccgat catctcgcct aattcagccg gacgtccgtt accttcacca
    20881 cgccagaagg ggagccgggc agaacgaccc ggagcaggag tcacaatcac ctgatcgcgg
    20941 gtgatttgct ggatccgcca tgaggtagcg ccgagagtga taatgtcgtt cacccgcgac
    21001 tcatatacca tctcctcatc cagttcaccc acccgccgcg aaccggcctt ttcttcacct
    21061 tcgggtaata acacgctata catgccacga tccggaatgg tgccgccgct ggtaaccgcc
    21121 aacaattgag cgccaggtcg ggcggtcaat atcccggtct ccctgttcca gaccagtttg
    21181 gggcgaaaag cagaaaaatc gccagaggga tagcgcccgg aaagcatatc cagcgtggcg
    21241 tcaaaaacac gtcttggcag atctttccac ggtgcggcac ggcgtacgcg ggagtaccat
    21301 tcgtctacct gtaatgcatc catcgccgcg gcggcaacgg tttgctgcgc aaggacgtcg
    21361 agaggattat gcggtggtgt caggttttcc agcctgcctg cgaacataca ctctacaatg
    21421 actgcggaat cgactaaatc acgccgggta cgggggaaaa acagcccttt agatacaccg
    21481 ccaacctgat gtccggcgcg accaatgcgt tgtaacccac tggcaacaga aagcggcgtt
    21541 gcctcctgaa tcaccagatc caccgcgccc atatcaatcc ccagttcaag actggaggtt
    21601 gcgaccacgc agcgtaattc acccgatttc agcgcctgtt cggtgattgc tcgttgttct
    21661 ttagagacgg agccgtggtg tgagcgggca ataaaaacgt cgctactttg tacacggtta
    21721 gaggttgcgc cggaggtcga ctcgaaatgg gccgcatcaa cggcgataga cggggaacgc
    21781 tgtaagcgtg cggcgtaaag ctcatttaat cgtgccgtca gtttttccgc cagcccgcgt
    21841 gaattagtaa agacaatggt cgagcgatgg cgcaacactt catcaaggat acccgtttca
    21901 atatatggcc agatggagcc ttcccggccg gcatggctgt cttcgccggt gccgctggcg
    21961 accgatgaga catcatccat attggcgacc ggtacgacaa ttcgtatctg cggatggcgc
    22021 attgcgggcg ggttgactac cgtaaccggg cgatcgccac caagaaatgc tgccacatcg
    22081 ctggctgagc gcacagtggc agaaaggcca attcgctgtg ctgaggtgtg gagcagcgca
    22141 tcgagccgct ccagacttaa cgccagatgc gcaccacgtt tactgcccgc taccgcgtgg
    22201 acttcatcaa taattaccgt ttcgacgccg tgtagcgttt cgcgcgcgcg ggaggtcagc
    22261 atcagataga gtgattcggg tgtggtgatc agaatatccg gcggattacg ggtgagtttg
    22321 ctgcgttcct gtgcaggcgt atcgccagta cggatcccta cgcgaagatt gacttccgtt
    22381 tcgccgcgcc gccgccgttc atcggcaata cccttcaacg ggatctgcaa gttgcgctga
    22441 acgtcggtgc ccagggcttt tatcggtgaa atatagagga tgcgtgaggt tttacgctta
    22501 tgcgcctcgc gggtatcttc gccgccttcg cggaagagcc gatcgagggc gtagagaaat
    22561 gctgccagcg ttttcccgga gccagtcggt gcaatcacca gcgcatgttc gcctcgcgcc
    22621 gccacatgcc aggtttgcga ctggacagcg gtcggctgtt taaaggcgcg aagaaaccag
    22681 tcgcgggtcg ccggtgaaaa cacgtccggc aggagcgatg aagggtctgg attatctgcc
    22741 attaacgcta ttccccgtga atatcatgct tgatgcgtgg aatagcctca ctatagatgc
    22801 ctgaatcatt gcatgtcatc aggcatcgac tcgattacac ctcttcggcg gcaggtagcg
    22861 gccagcctcc cagacgtttc cagcggttga cgatttcaca aaacagcaca gcagtgcgtt
    22921 cggtgtcgta cagcgcggag tgcgcctggg tgctgtcgaa gtccatgcca gcggtctggc
    22981 aagcctttga caatacggtt tgtccgagtg ccagcccggc cagtgcagca gtgtcaaaag
    23041 tggcgaaagg gtggaacggg ttacgtttca gcgaggcgcg ttctgcggcg gccatcataa
    23101 agctgtgatc aaaattggcg ttgtgcgcca ccataatggc gcggttacag ccgctcgctt
    23161 taataccttt acgtacaact ttaaaaattt cgtgcagcgc ctcgtattcg ctgaccgcgc
    23221 cgcgatcggg atcgttcggg tcaatgccgt tgaaggcgag ggcttctggt tgcaaatttg
    23281 cgccgacaaa tggttcgacg tggaaatgta atgtggtgtc cggcatcagc cagccttgtt
    23341 catccatttt cagggtgatg gcggcaatct caagcagcgc atcggttttg gcgttaaatc
    23401 cggctgtttc aacatcgatc acaacaggat aaaaaccacg aaaacggtcg cacagaccgg
    23461 taagttgagc gttatcggac atctgattct cttaatacgg ataaattgca gcgcgcatta
    23521 tgacaaatat tacttgtcga tgcagtaaag atgcgggcgc gatgagttca cgcccggcag
    23581 gagattagtt gcccagaccg cgaccggcgt ctttctcttc gattaactca attttgtaac
    23641 cgtccggatc ttccacaaac gcgataaccg tagtaccgcc ttttaccgga cccgcctcac
    23701 gggtcacgtt acccccgttt tgacggattt tttcgcacgc ttcagcggcg ttatctacgc
    23761 taagcgcgat gtgaccataa gcagtgccga gttcgtattt atccacgccc cagttgtagg
    23821 tcagttcaat caccgcttct tcggtttccg ggccgtagcc aacaaacgcc agtgagtatt
    23881 tgtattccgg gttttcgctg gtacgcagca gtttcatgcc cagcactttg gtataaaaat
    23941 cgatggagcg ttgcaaatcg ccaacgcgca gcatggtatg aagaagacgc atctttttat
    24001 cctcaaaatg gctaacagat tcaaaatgtt gttttagtat agcggcggct ttacgccgct
    24061 cgcaatgttg gattacaacg tcgggtaatc ggtatagcct tccgcgccgc caccgtagaa
    24121 actttcggca cgctgtgggt taagctcagc tttgagctgc aagcgggcga ccagatccgg
    24181 gttcgcaatc cagtcacgac caaatgccac cgcatcaatt aaccctttgc cgatcagcgt
    24241 ttcagctttt tctactgtgt atgcacctgc gccgataatc ggaccgtgga aacgggcgcg
    24301 tactttttcg cggaacgcat cagtatacgg ttcacccccc gcccaatctg gttctgacat
    24361 atgcagataa gcaatgccgc gtttacccag ttgttcaatc agatacagtg catcggcttc
    24421 ttcattcggg ccgttatctg tgttctggaa agtaccgatt ggcgaaacgc gaatgccaat
    24481 gcgatcggca ccccattctt caatcccggc atcgaccact tccagtacca aacgtgcgcg
    24541 attttccacg ctgccgccgt actgatcggt acgatggttt gaagaagggg aaaggaactg
    24601 atgcagcaaa taaccgtgag cagagtggag ctctaccaga tcaaaaccgg cttcacgcgc
    24661 gttagcaatg gcctgacgga aatcattgac gatacccgga atctcttcca gttcaagcgc
    24721 acgcggcatg gatgtttcaa cgcggatcgc ccgaccattt tcatcgcgca gagaagtacg
    24781 tgttcccgcg ctaagtgctg aaggcgctac cggtgcctga ccgccaggtt gcaggctggc
    24841 gtgagaaatg cgtccggtgt gccacagctg caaggccata tgaccatttt cagcatgaac
    24901 gccagcggtg atttttttcc atgcggcaat ttgctccgga ctatggatgc caggcgcacc
    24961 tgcatatcct tttgcctggg cagaaatttg cgtggcttca ctaataatca aaccggcact
    25021 ggcacgttgg cgatagtatt ccgccatcaa cggggtagga atgtcacccg gttcaatact
    25081 gcgcagacgc gtcagcggtg ccataaaaat acggtttgcc gccgtgatcg cgcccacttt
    25141 cagtggggaa tacagttttt cagatgacat aaaggctccc ggtcgtctgg tgataaaaaa
    25201 tgcctgctaa acggcaggcg tcgcaataat gttttttaca tgggccagcg cgttttccag
    25261 tggctcgaaa ctgcgcgaaa ttttggcctg cagattcgcg ccaagccata gtgcgtaaag
    25321 cacttgtgcc tgttgtagcg gttcgccaca aaagattaaa caatggttct cacggccatt
    25381 ttccagagcc tgcgagagca gggcgatcac gccgcgagca cctttatcca tcgcgctgcg
    25441 catatcttct gacagatcgc acacttcggc agagagtttt actgtcaggc aaccactgat
    25501 ggttccatgt tggcaaaact ggttcagtgt ttgctggtaa taagccagta tgcggtcgcg
    25561 gtagttacct tcgccggatt gcagcaactc agccagtcgc tggtgatatg cggcgtaatg
    25621 acgctcaagc atggcaacgc caaacgcttc tttagagcga aagtagtgat agaaggaccc
    25681 tttcggcact tcagcggttt ttagtaattc gcttagcccc atcccggtga atccacgttg
    25741 caggcaaagt tgctcgcccg tcgccaagag atgttcgcga gtatcatgtt cggtgtgttt
    25801 gttcatgcgt tggagtgtag tagaccagtc ggtctagtgc aagcaaagtt gctactttgg
    25861 acattaacag cttcaataat ggtaataatc ttatctgagg agggcgaggt ggcggagcaa
    25921 ttagagttct ttcccgtcca gagcccgtgc cggggaattt gccagtctga cgaacgcggg
    25981 ttttgtcgcg gctgttttcg tagtcgggat gagcgtttta actggaataa aatgagcgat
    26041 ggcgaaaaac aagaggtgct gcgcctttgt cggcagcgat taatgcgcaa attacgcgca
    26101 aataagccag cgtcatcaga cgaaccggag caaccatcac tcttttgaac ctaatcttgc
    26161 gtatactcct ctcatatctg ataagaggaa gcgattatgg ttcagcgtat tactattgcg
    26221 ccggaaggcc cggagttttc ccgttttgtg atgggctact ggcgattgat ggactggaat
    26281 atgtccgccc gccagctggt cagttttatt gaagagcatc tggatctcgg cgtgaccacc
    26341 gtggaccatg ctgatattta tggtggctat cagtgcgaag cggcgtttgg cgaggcactg
    26401 aaactggcac ctcacctgcg tgaacggatg gaaatcgtca gtaaatgcgg tatcgcgacg
    26461 accgcgcgtg aagaaaacgt cattggtcat tacatcactg accgcaatca catcattaag
    26521 agcgccgaac agtcgctaat taatctcgcg accgatcatc tggatctgct gttaatccac
    26581 cgaccagacc cgttaatgga tgccgatgaa gtggcggacg cgttcaaaca tctgcatcag
    26641 agcggcaaag tgcgtcattt tggcgtatcg aactttacgc ctgcgcaatt tgccctgttg
    26701 caatcacgtc tgccgtttac ccttgccact aatcaggtgg aaatatcccc ggtgcatcag
    26761 ccgttactgc tggatggcat gctcgaccaa ctacaacaac tgcgtgttcg tccgatggcg
    26821 tggtcctgcc ttggtggtgg tcgtctgttt aatgatgatt atttccagcc gctgcgtgat
    26881 gaactggctg tggtggcgga ggagttaaac gcgggctcga ttgaacaggt ggtttacgcc
    26941 tgggtattac gtttaccgtc gcagccgctg ccaattatcg gctcaggtaa aattgagcgc
    27001 gtacgggcag ctgtcgaagc agaaacactg aaaatgaccc gtcaacaatg gtttcgtatc
    27061 cgtaaagctg cactggggta cgacgtaccg taagcacttt taggaatagc cgccgttcaa
    27121 aaatgtgtca ctggtttaca cttattcagg aatgcacaat gaacggaggt cctatgaaac
    27181 gttttagtct ggccattctg gcgctggttg ttgcgaccgg cgcacaagct gccagtgaaa
    27241 aagtcgagat gaacctcgtc acgtcgcaag gggttgggca gtcaattggt agcgtcacca
    27301 ttactgaaac cgataaaggt ctggagtttt cacccgatct gaaagcatta ccccccggtg
    27361 aacatggctt ccatattcat gccaaaggaa gctgccagcc agccaccaaa gatggcaaag
    27421 ccagcgccgc ggaatccgca ggcgggcatc ttgatccaca aaataccggt aaacatgaag
    27481 ggccagaagg taccgggcat ttaggcgatc tgcctgcact ggtcgtcaat aatgacggca
    27541 aagctaccga tgccgtcatc gcgcctcgtc tgaaatcact ggatgaaatc aaagacaagg
    27601 cgctgatggt ccacgttggc ggcgataata tgtccgatca acctaaaccg ctgggcggtg
    27661 gcggtgaacg ctatgcctgt ggtgtaatta agtaattacg aggccagcgt accttgcggt
    27721 ggtgcttgct caagttgcga aagcgagcag tacagccgcc agacaattgc cgccagctca
    27781 cgggcggcag gttgatgatg acgggcaagg ctgtcgcaaa tccgctgtaa ttcttcaagt
    27841 gtggccgcca gtgatttttg ctgaacgcca cgctcactca tcactccccg caacagtgaa
    27901 atacagttat cccgtactcg cgataacgga tcggaacgcg attcccagtc gcgcaattgc
    27961 cagacaacat gagaacagtt cagcagcact acaccccagc gtaataacca acggcgagcc
    28021 agcgcatcct ggctgttact caactgactg acgtgatgat aagtgagcga ttcaaattcg
    28081 ctttcactca gtgttggatg gcggcttagc tgatcgacaa aatcccggcg cagcgcgcga
    28141 atatggcggc ggcttttacg agcatccgat cctggacgca gaatggcgaa cgctagccaa
    28201 gccagcgcaa cgccaacgat ttttgccaga ttatcgttaa gaaaatcagc aaaatcatac
    28261 accgggggat tagtgacagc gataaacgaa cccataaaaa caatcagttg cccccacagt
    28321 gcggcaaatt tgggcatctg caatttaaga agctgcattg tcgccagcag tggaaagaga
    28381 aacagtaaaa attgccacag atcgctaatc tggaccatca ggccaaattt gaccacaaag
    28441 ctgaatagcg aaagtaacac cagcgtgcgc atcagcagtg acaacgactt aaacggtgct
    28501 gcgacggcgg agtagagtac gcagctaatt gctgccagcg ttaatgcatt ggcaccggca
    28561 tcccattgcg atgcaatact ccatgcgcca atcatcatca acgtacaaaa tgtacgcagc
    28621 ccgctccaca tagcttcggc gttgtcggta tgacgagcca gagcggatgt ccgtgcgagt
    28681 ctggcatgat catctacacc gctttgcaga cgatgtaatt cctgactact ttgcagataa
    28741 aggcggcaaa aataacgtag tcgctgccag aaggcgacgt gccggtagtc ggcgacgttg
    28801 gtcgggcgta gcggggcgat aatacgtgcg acggtgtaag catctgtttg cgaactggcg
    28861 agcgccgtca gtaattgttc gagaatttct cgcgtggcac ctggcggtga gggccagttg
    28921 agcaacatac gtcgcaggct ggagatgaca ctggtcatac gtaattgctg gtggagtagc
    28981 gcattaaggc gcgcgttttg ctggcgaaaa cgatagtggc tccagaaagc ctggatacgc
    29041 agcaaattca tggtcagtat ctgcccaatc accccctcat gtgctgcacg aatggcatcg
    29101 tttgtttcag gctgccagag taaactggca tgttccagta atcgggcgtg catgtttttc
    29161 aatgcagtta aaagggcagt agcatcggaa ctgctaggca ggatcatcat catcatgccg
    29221 ccgcacaaaa taccgactat cacctcgcaa acgcgcgcct gagcgatatc ccacagctgg
    29281 ctggcctcag taatattaac catcggaaag gcgatgatgg cagccgtgta gcccgccagt
    29341 tgaaatgcat acgcgacgtt attcgtgaag tgcgcacagg cctaggtaca aaagccaagc
    29401 cacgccgaca tgctcaatag aaaaaaccac ggctcattga gcgtatgtcc ggcaagaagc
    29461 agtgccgcaa tggctcccag taaactgcca gcgatgcgtc cgaggctttt gctgatgaca
    29521 ccgccaacgg tgggaaagct aaccactgca gccgaggtca tcgcccaata gggttcatcc
    29581 agattcaaat aataggcaac cgtcagcgcc agacacatgg caatggtatt gcgtaacgca
    29641 taacgccatt gcgccagcgt ggccctgaac cagcgcaaag cggacgggaa cgcgttgggc
    29701 gagacgaacc caggggacgt taggtttcac atccggaata aggcttgagt cactttcaac
    29761 gctctgatca taaatcgccc gaccgatact ggaaacgtga ccctgtaacg ttttgttatc
    29821 actatagagg gtaatttgtg ctggtgcacc ttcgcggata tggcgcaact tggtttcttc
    29881 aaaataacca atgacgtaaa acgagtggct gtcgacaagg gcaaacagcg gtttcccggt
    29941 gtcagcgtaa tcgccgatgc gggtagtgag gttagtcacc catccactca ccggggcgcg
    30001 aatttccgtt tgcgccagtt gccattgtgt ctgtttcagc gtcgcctgtg cggcattaac
    30061 actggcctgc atcgctttaa cattgaggtt agcggtatcg agttcttcgg caaaaataaa
    30121 attttgtgag agatgacgac gacgattcgc ttcgttattg gcttttgcga ggtcagattg
    30181 cgctttcgcc agctgagcct gtgcgttcag ctcggcgatc tgaaagggcg ttttatcgat
    30241 cgtgagcaga agttcccccg cgttaaccag ttggttgtct tttatattca gctcaacaat
    30301 acgtcccgac acctgtgggg tgatagaaac ctgttctgcg tgtattttcc cgtcgcgtgt
    30361 ccacggagat tgcatgtaat aattccacag ccaccatccg gcaagaaccg caactatcgc
    30421 tacaatgatt gttgagaaat acttaattgt tttaattgac atagttacca cgcaatcagt
    30481 attgccagag caaggcaaac gcaaatcgca aacagcgata aatccattaa caagggatgc
    30541 cagatgtcac cggcgtagat ccagccacga agcagacggt gtacgacaag ccagatgacg
    30601 aatccaaacg cgaatgcttt gaaaaacgga ggaaaataga cggacgcacc gaacaccaga
    30661 tcttgtaagg gcaatcctgt tgcattgagc ataaacttca caaagtagat tcctttacga
    30721 ccgaatatat tgcgttagat taataaatat tctttaagtg cgaaaaattt acgcgcaatt
    30781 tctgaaaaat atgtctgacg gtaaccaaat gcagcaatac atttgtttta gcaatacaat
    30841 tgctgcacac tattctaaaa gccgcataat atcttagcaa gctaattata aggagatgaa
    30901 attggaatcg ccactaggtt ctgatctggc acggttggtg cgcatatggc gtgctctgat
    30961 agaccatcgc ctgaaaccgc tggagttaac acaaacccat tgggttacgt tacacaatat
    31021 ccatcagtta cctccagacc agtcgcaaat tcaactggca aaagcgattg gcatcgagca
    31081 gccatcactg gtccgtactc tggaccaact ggaagaaaaa gggttaattt cgcgtcaaac
    31141 ttgtgccagc gatcgtcggg ctaaacgtat taaactgacg gaaaaggcag agccactgat
    31201 cagcgaaatg gaagctgtta ttaacaaaac ccgcgcggaa atattacatg gcatctccgc
    31261 agaggaactg gagcaactga ttacgctcat cgcaaaactt gagcataata tcattgagtt
    31321 acaggccaaa gggtgaaatg aagggggcgt gtggtcagta acctgaccac acgcaaactt
    31381 atttagcgcg gagaaacggt cacctgactg ccattgctgg ccagtacgac acgttggccc
    31441 ggagagaaac gagtgttgcc ttgtttctgt accaccatga tggtattacc atcgtcttta
    31501 cgaatttcca gctcgacacc ctgcgttttg ttcattgcac tctgtacgcc ctgaccagct
    31561 acgccacctg caacagcgcc tgctgcagta gccagagaac gcccggttcc gccaccaaca
    31621 gtattcccca ggaaaccacc aagaacagca ccgccaattg caccgataac gttggaatca
    31681 tcaccgccct gaatctgtac cggacgtacg ttaacgatgg tgccatagct gacattctgt
    31741 acttgtttcg cttcagaagc ggtataaaca tcccctgaca gggtgtcgtt attaacacaa
    31801 ccgacaagag acagacctac cattgaaaca accaatacgc gtttaatcac tgaaaaatct
    31861 cctgttcacc atgaaacgcc acgcaagcat ccctcatggt caaagtatat ggcatatgtg
    31921 aatccatggt tacatgttct accaaatatg agcaaatcat aatgaaagta ggatcaacca
    31981 attataaaca aaacattcat gatgattatt gatttgaaga taatgtttta ctaccaacgg
    32041 taaaaatcgg tcattccgca tggtattcac gaaaagttta tgtttgagtg gtgcgcaaac
    32101 gtcagattga aaaaggatga actatgaaat cgggccgctt tattggcgtt atgtcaggca
    32161 ccagccttga tggtgttgat gttgtgttgg cgacaattga tgaacaccgg gtcgcacagc
    32221 tggcaagctt gagctggccg atcccggtgt ctctgaaaca ggctgtactg gatatttgcc
    32281 agggccagca gcttacactt tcgcagtttg gacagcttga tactcaactc gggcgacttt
    32341 ttgctgatgc ggtcaatgcc ttgcttaagg aacaaaacct gcaggcgaga gatatagttg
    32401 cgatcggctg tcacggtcaa accgtctggc atgaaccgac gggcgtggca ccacacactt
    32461 tacagattgg cgataacaat caaattgtgg cacgcaccgg aattacggtt gtcggtgatt
    32521 ttcgccgtcg cgatattgcc ttgggaggac aaggcgcacc gctggtacct gcgttccatc
    32581 atgccttgct ggctcaccca accgagcgac gaatggtgct caatattggc ggcatcgcca
    32641 atctgtcact gctcattcct gggcagccgg ttggcggcta cgataccggt cctggtaaca
    32701 tgctgatgga tgcctggatc tggcgtcagg caggtaaacc ttacgataaa gatgccgagt
    32761 gggcacgggc gggtaaagtt attctcccac tgctgcaaaa tatgctcagc gacccgtatt
    32821 tctcgcaacc tgcaccgaaa agcaccggac gcgaatactt taactacggc tggctggagc
    32881 gccatttacg ccattttccg ggtgttgatc cccgtgatgt tcaggccaca ctggcagaac
    32941 tcaccgccgt gaccatttct gaacaagttt tgttgagcgg tggctgcgaa cgattgatgg
    33001 tatgtggtgg aggtagtcgt aatccgctac tcatggcgcg tctggcggta ttactgctag
    33061 gcacagaagt caccaccacc gatgccgttg gcattagtgg cgatgacatg gaagcattgg
    33121 ctttcgcctg gcttgcctgg cggacgctgg cgggattacc aggaaatctg ccttccgtca
    33181 ctggcgcaag ccaggagacg gtactggggg ctattttccc cgccaactcg tgatatcaga
    33241 attaactgaa ttttttctta tcgtgtctgc cgttacactt ggaagattaa ggagggtatt
    33301 tgccctccgg accaggaaag tcttcgggat atgactatga aaaaactgtt aattatcatt
    33361 ttgccggtcc ttctctccgg ttgtagcgcc tttaatcaac tggttgagcg aatgcaaacc
    33421 gatacgctgg aataccagtg tgatgaaaaa ccgttgacgg tcaaactgaa taatccgcgc
    33481 caggaagtca gttttgttta cgataatcaa ctactgcatc tcaaacaggg cttttcagcc
    33541 tctggcgcgc gttactccga cgggatctat gttttctggt cgaaaggtga ggaagcgact
    33601 gtctataaac gcgaccgcat cgtcttgaat aactgtcagt tacaaaatcc acagcgttga
    33661 gatttttcca ggggcggcgc acaatagcgt cacccactga caatccgtaa agaaaaccat
    33721 gtctgataac gacgaattgc agcaaatcgc gcatctgcgc cgtgaataca ccaaaggcgg
    33781 gttacgccgc cgcgatcttc ccgccgatcc attaaccctt tttgaacgtt ggctctctca
    33841 ggcttgtgaa gccaaactgg cggaccctac cgcgatggtg gtcgctaccg tggatgaaca
    33901 tggtcagcct tatcagcgca tcgttttact caaacattac gacgaaaaag gcatggtgtt
    33961 ttacaccaac ctcggtagcc gtaaagcaca tcaaattgaa aataatccgc gcgttagcct
    34021 gctgttcccg tggcataccc ttgaacgcca ggttatggtg atcggtaaag cagaacgact
    34081 ttcgactctc gaagtgatga aatattttca tagccgcccg cgtgatagcc agattggtgc
    34141 atgggtttcg aagcagtcca gtcgcatttc tgcccgcggt atccttgaaa gtaaattcct
    34201 ggagctgaag cagaagtttc aacagggcga agtgccattg ccgagctttt ggggcggttt
    34261 tcgcgtcagc cttgaacaga ttgagttctg gcagggcggt gagcatcgcc tgcatgaccg
    34321 ctttttgtac cagcgtgaaa atgatgcgtg gaagattgat cgtcttgcac cctgaaaaga
    34381 tgcaaaaatc ttgctttaat cgctggtact cctgattctg gcactttatt ctatgtctct
    34441 ttcgcatctg gcgaaaagtc gtgtaccggc aaaggtgcag tcgttatata catggagatt
    34501 ttgatggcaa gcagtaactt gattaaacaa ttgcaagagc gggggctggt agcccaggtg
    34561 acggacgagg aagcgttagc agagcgactg gcgcaaggcc cgatcgcgct ctattgcggc
    34621 ttcgatccta ccgctgacag cttgcatttg gggcatcttg ttccattgtt atgcctgaaa
    34681 cgcttccagc aggcgggcca caagccggtt gcgctggtag gcggcgcgac gggtctgatt
    34741 ggcgacccga gcttcaaagc tgccgagcgt aagctgaaca ccgaagaaac tgttcaggag
    34801 tgggtggaca aaatccgtaa gcaggttgcc ccgttcctcg atttcgactg tggagaaaac
    34861 tctgctatcg cggcgaacaa ctatgactgg ttcggcaata tgaatgtgct gaccttcctg
    34921 cgcgatattg gcaaacactt ctccgttaac cagatgatca acaaagaagc ggttaagcag
    34981 cgtctcaacc gtgaagatca ggggatttcg ttcactgagt tttcctacaa cctgttgcag
    35041 ggttatgact tcgcctgtct gaacaaacag tacgatgtgg tgctgcaaat tggtggttct
    35101 gaccagtggg gtaacatcac ttctggtatc gacctgaccc gtcgtctgca tcagaatcag
    35161 gtgtttggcc tgaccgttcc gctgatcacc aaagcagacg gcaccaaatt tggtaaaact
    35221 gaaggcggcg cagtctggtt ggatccgaag aaaaccagcc cgtacaaatt ctaccagttc
    35281 tggatcaaca ctgcggatgc cgacgtttac cgcttcctga agttcttcac ctttatgagc
    35341 attgaagaga tcaacgccct agaagaagaa gataaaaaca gcggtaaagc accgcgcgcc
    35401 cagtatgtac tggcggagca ggtgactcgt ctggttcacg gtgaagaagg tttacaggcg
    35461 gcaaaacgta ttaccgaatg cctgttcagc ggttctttga gtgcgctgag tgaagcggac
    35521 ttcgaacagc tggcgcagga cggcgtaccg atggttgaga tggaaaaggg cgcagacctg
    35581 atgcaggcgc tggtcgattc tgaactgcaa ccttcccgtg gtcaggcgcg taaaactatc
    35641 gcctccaatg ccatcaccat taacggtgaa aaacagtccg atcctgaata cttctttaaa
    35701 gaagaagatc gtctgtttgg tcgttttacc ttactgcgtc gcggcaaaaa gaattactgt
    35761 ctgatttgct ggaaataatg cattaagtgg aaagggggag tgagaaatca ctccccctgg
    35821 tttttataca gggaacatga tgaaaaatat tctcgctatc cagtctcacg ttgtttatgg
    35881 tcatgcgggt aacagtgcgg cagagtttcc gatgcgccgc ctgggcgcga acgtctggcc
    35941 gctgaacacc gttcaatttt ctaatcacac ccaatacggc aaatggactg gctgcgtgat
    36001 gccgcccagc catttaaccg aaattgtgca aggcattgcc attgccgcca ttgataaatt
    36061 acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg gtgaacatgt
    36121 cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt tttgcgatcc
    36181 ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag agtttcatgt
    36241 acggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc tggaaatact
    36301 ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg aactcattga
    36361 gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca gccgtgaccg
    36421 ttttgaaatg ctgctggtca ccgccgatga agcctggcat atcagccgtc cactggtgga
    36481 ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac tgctggtgaa
    36541 actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg cagtctacga
    36601 aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg ctgctcagga
    36661 tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata tctcatttcg
    36721 gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga cagcgcgtct
    36781 tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc taacccttcc
    36841 agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc atcagcaatt
    36901 gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac atattgcagc
    36961 ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc tgggcgaaac
    37021 agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc gatggtttta
    37081 tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct gtcggcaaga
    37141 tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag caatgcaggc
    37201 acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg ttttttcatt
    37261 aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat atgggaagcg
    37321 agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg tctgtggttt
    37381 aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt tagcggcttg
    37441 taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc tgcagcgtca
    37501 tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc aataacggca
    37561 gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat cagcgggtcg
    37621 gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag gtttgcaccg
    37681 gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg aacgagttgt
    37741 gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg caggccatag
    37801 cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt cgcacccagc
    37861 ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt ggttggcatc
    37921 ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc gatgatgatc
    37981 cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag aatggagtgc
    38041 tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta cagcacgaag
    38101 aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg cgcagcacct
    38161 ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac aacgcccagc
    38221 gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat cagcgccaca
    38281 acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc tggttttgaa
    38341 ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat cagcaggcct
    38401 acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca cggggtggct
    38461 atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa tgcaccgtcc
    38521 agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt agctttaaac
    38581 aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc gtcgtgacca
    38641 gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat aattacgcgt
    38701 ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc ataaaccagg
    38761 gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag ttgtttaacc
    38821 aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca taactcaatc
    38881 gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac gctttcagtt
    38941 ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat taacgggggg
    39001 gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga ggggaaacgg
    39061 caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc tgttacatat
    39121 aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa tttttcaaaa
    39181 aacgtaacgg cagatttttt cactgcattg agagggggat gatggcttta cggagatatg
    39241 ttctgctatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat agccagtgta
    39301 atgttttgca ggtcaatatt tgttgcgtgt gtaaatttaa atcattttaa cctgattctt
    39361 gcaatgtttt tacattgcca cagcatcgtg atcttgatca cgaatgaata gaaaatttcg
    39421 ctattaaaaa aacaattaac ctgcattttt ggttatccgc aaacggatta cctgatgata
    39481 acgctaagca atggcatcat tgacgttaaa aagatgtttt aaaagaggat aaagaaaggt
    39541 tatcaatggg gtaatcggtg ttaccccttt tcttcagatg tcaactctct ctttgtattc
    39601 acaaagatct tcaataatac aagagccaca gcggggcttg cgggcaatgc aggtataacg
    39661 cccgtgcagg atcaaccaat ggtggcagtc gactttaaac tctgccggaa ccactttcag
    39721 tagcttttct tctacctgtt cgacgttttt ccccggcgca aattgagtac gattacaaac
    39781 gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg aatgcagtgt ttaatacgac
    39841 gttggctgtt ttacgaccta cgccgggcag ggcttcaagc gcagcacgat cttccggaac
    39901 ctcgccatta tgttgttcca gtaatatacg gcaggttttg atgatatttt ctgctttgct
    39961 gttataaagg ccaatcgttt tgatataggt tttcacccct tcaacgccca gttcaagcat
    40021 cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc gccttattaa cactgatatc
    40081 ggtcgcctga gcggaaagca gtacggcaat cagcaattca aaaggcgaac tgaaattaag
    40141 ctcggtggtg ggatgaggat tgttctcacg caggcgagtg aggatctcca gacgttttgc
    40201 tttgttcatc agacattccc tgtttcaccg tttggcaatg cacgttcggc agctgcttca
    40261 gcacggcgct ttttcattct ttcatcaatc aggtattttc ctgccagcat cagtcccaag
    40321 ccaataaatg cacctggtgg cagcatcgcc agcaggaaag gggagtcggt gtggaaaatc
    40381 tccacgcgta atacttttgc ccagctacct aacagcgcat ctgcaccgtc aaacaacgtg
    40441 ccattgccga taatttcgcg tagtgaaccc agcacgaaca tggcgcaggt tgcgcccgta
    40501 ccaattgaaa agccgtccag tgccgaaagc gccggacctt ttttggcggc gaaggcttca
    40561 gcgcggccca caacgataca gttagtgaca atcagcggaa taaaaatccc taatgattga
    40621 tacaggccaa aggcgtaggc gttgatcagc atctgtacag cgctgaccac cgaggcgatg
    40681 atcatcacgt aaatgggaat gcggatctcg gctggcgtcc agtgacgcag cgtcgaaatg
    40741 gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc ctaaacccag agcgttagtg
    40801 gcagtggacg tgaccgccaa tagcggacaa aggccgagca gttggaccag tgcagagttg
    40861 tttttccaca acccctgaac aataacgtct ttaatttcgc tcacgcttta ttctccacag
    40921 gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt acaatccggc gcgttttacc
    40981 gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga actggtcgaa atcaccacca
    41041 tctttcttca ccgcccagtg cgcatcatcc gcaccactga ttttttttcc cgcaaaatgg
    41101 gtgagccagt cagaaaggcg caattcgatt ttatcgccaa gccctggcgt ttcgtggtgc
    41161 tctgtcacgc gcgtgccaag taccgtgcca ttaaaatcgg ctcccaccag cagctgaatc
    41221 gcaccggaat agccatctgg cgcggttgct tccagaacgg cggctaccgg tttgtcatcc
    41281 tgtttggcga tgtaaacccg atgctcacct ttacctaact ctggcgcagt taccagatag
    41341 caactctgtg cgagcgcatt gttatagcgt tcggctggca gcacctgacc aaataacgcc
    41401 ttttgttgca gactggcctg ttcagcaatt gtcgttttgg tcatctggtt gatggccgca
    41461 gttaaccctg tagaacccgc tgcaaatagt gccagtgtaa tgccgtgttt tcggatagtt
    41521 ttcagcatgg tttacccttt gcgatggccg tagacgcgcg gacgcgtgta gtaatcgatc
    41581 agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca cgccgtcagg atagccgccg
    41641 aaactgcgga tcaaccagac taataagccc gccagcgcgc cgaacatcag ccgaccacga
    41701 ttggtcgtag aagcagtaac tgggtcagtc aaaataaaga atgcgccgag catagtcgca
    41761 ccagacagca gatgaatttg cggtgctgcc agtgtttctg gtgagaacaa ccagcccaac
    41821 gttgcgcata acgccagcgt tactaagaag ctgaggggaa tatgccagcg aatcgctttc
    41881 tgccatagca accacacgcc accagccagc caggcgagat ttacccattg ccaaccagcg
    41941 cccgccagaa taccgctgta gatcggatat tgcataatct gttcaaccga atgacctgcg
    42001 cggacagagg ttttgaatgt atccagcggt gtcgcctgac taatgccatc aatacctaag
    42061 cgtagcgtat tcatatcacc accactggcg gtatgaccgc tgaaaataac ctgtatggcg
    42121 tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg gtaaccagct ggtcatctgc
    42181 accgggaagg agatcagtaa gaccacataa ccaatcattg ccggattaaa cgggttttgc
    42241 cccagaccgc catacaactg tttagcgatg atcaccgcaa acaccgtacc cagtacgacc
    42301 atccaccatg gcgctagggg gggaatactt accgccagca ataagcctgt cagcaatgct
    42361 gagttatctt tcaacgttgc ggctaccgac tgcttgcgta gtttgagtac gagagcttcg
    42421 gctaacagag cactgaccga cgccaacagg atctgaacga gagtacccca accaaaaaac
    42481 cacagttgcg ctgcgattcc tggcacggct gcgagcaaca ccaacagcat aatgcgcgat
    42541 gtctggcgct ggttatgggt ataaggggag ctagctattc tgaataccat ttagtcctcg
    42601 tttacaacct tttgctgggc ggcttttttg gcctgaacgc gggcaatagc cgcggcaact
    42661 gcggctttgc gcggatcgac ctgttcttct ggttccgcat tagcctgttg ctgttccagc
    42721 ttgcgtgctt tggcgcgggc aatagcggct tcgacggcag ctttgcgcgg atcaacctgt
    42781 tcttctggct ccgcattggc ctgttgctgt tccagcttgc gtgctttggc gcgggcaata
    42841 gcggcttcga cggcggcttt gcgcggatcg acctgttctt ctggttccgc attagcctgt
    42901 tgctgttcca gcttgcgcgc tttggcacgg gcaatagcgg cttcgacggc ggctttgcgc
    42961 ggatcgacct gttcttctgg ttccgcatta gcctgttgct gttccagctt gcgcgctttg
    43021 gagcgggcga tagctgcttc aacggcagtt ttacgtggtt cagcaacggt tgctgcgtcg
    43081 ttagtttgct ccagttctgc ctgtttcgct ctggcttgcg ctttacgggc ttcccgtgct
    43141 gcaataattg cactgttatc cgggcgttcg cccgctttaa tcacaatagg ctgtgtagcc
    43201 tgggcctgtt tctctttcac ccgcgccaga gcggcagcaa tcgcatcttt atctttggct
    43261 gcaggttgaa cggctgcgct cttatgtcgt tcaaggcgag cagctttttc gcgctccaga
    43321 cgagcctggc gcgcttcgaa acgcgctttg gcttctgcgg cgcgcttttc ttcctgacga
    43381 atagccgcaa tttcagcttt ttcctgacgg aaatattgca ccaagggaat attgctcggg
    43441 caaacccacg cgcaagcccc acattcaatg cagtcggcaa tgttatgcgt ggtagcttta
    43501 tcgtgttgct gacctttgct gaaccagtac aactgttgcg gcaaaagatc cacagggcag
    43561 gcgtcagcac aggcgctaca ccggatgcag ctttgttctt cctgtggttc gccaagttca
    43621 ttggcagagg gagccaacag acagttggta atctttacga ccgggacatc cagccatggc
    43681 aaggtaaagc ccattagcgg gccacccata atcaccattt gatcggcaga ggggcagaat
    43741 ccggcatcat tcaataaatg acgcactggc gttcccagcc gtgcccagac gttgcccggg
    43801 cgagcgattg cttcgccagt cagggttaca acacgctcgg taatcggctc gccatcaata
    43861 acggcacgtt tcactgcata agcagtgccg acgttttgca ttaatacgcc gatatcggat
    43921 gaacgcccgc catgtggaac ctgcttcccg gtcagaatgt aggttaattg tttagcaccg
    43981 ccagaaggat atttggttgg aatcacccgc agagaaatat cgttagagtc cgccagcacc
    44041 gcgcgcagca tggaaatcgc ctgcggtttg ttatcttcaa tgccgataag aatttcgcgt
    44101 ggctgcagaa tatgcgcaag aatgcgaata ccctctacga cctgagccgc gcaatcctgc
    44161 atcaaacggt catcggcggt aatgtacggc tcgcactcag cagcgttgat aatcaacgtt
    44221 tcaatcttat ctccaccacc ctgcaattta acgcctgtcg ggaatccggc accgcccagc
    44281 ccggcaacac caaactgatg tatgcgctcg attaactctt cgcgactgcg agagcgataa
    44341 tcgacccagc cgtcgcgcgg gatccagcag tcttcaccat cggcatcaat aatcacgctt
    44401 aattcagcta aagctgaagg atgagccgta gagtggggcg caatagccgt aacggtaccc
    44461 gaggtgggcg cgtgaacagg cagcattttg ccgcgaccac gggtaagcgg ctggccgcgc
    44521 aatactttat cgccgacgct aacgcacaac tcaccttcag cgccaatatg ctgtttcagt
    44581 ggaataacaa aacgctgcgc cagtggtacc tggcgcaggg gggtaccgtt ggactgggtt
    44641 ttcatctccg gtggatggat gccgccgttg aaatcccaga ttttattttt tctgaatgca
    44701 gagaataact taagcatggt gttccacggg aatgatacgc acgggaatgg tgttcagatc
    44761 ccatttccag gagtcaggtg tttctgcgac cggttgcaac gagatgcagt gcgtcgggca
    44821 cggatcaaca cataaattgc agcccgtaca gagatcactc attaccgtat gcatggcacg
    44881 ggtagcgcca acgatggcgt ctaccggaca agcctgaata catttagtgc agccaataca
    44941 gttattttca tcaataaccg ccaccatccg cgcaggcgtt agctcttgcg cttcgccatc
    45001 cagcggctgc ggctcgacat taagcaactc ggcaattttt agcatcacag cttcgccacc
    45061 tggggcgcaa cggttgattt tttcaccgtt acagctgatg gcttccgcgt aggggcgaca
    45121 gccgggataa ccgcactgac cacactggct ctgcggtaag atttcgtcaa ttttctcaac
    45181 gaccggatcg tcttccaccg caaaacggcg ggaggcataa cccagaatgg cgccaaacgc
    45241 caggcccagc aggctcacgg cggcaacggc aatccagata gcattcatta caacttcacc
    45301 aaaccactaa agcccataaa ggccagagac ataagacctg cggtaattaa cgcaatggca
    45361 ttaccgcgaa aaggtgccgg gacatcagcc acagcaaggc gttcgcggat ggcggcgaag
    45421 agcaccatca ccagcgagaa accgacagcg gcggaaaaac cgtacagcgc cgactgcaag
    45481 aaattgtgcc cgagattgat attcagcaac gccacgccga gcactgcaca gttggtggtg
    45541 ataagcggca aaaaaatccc cagcagacgg taaagcaccg ggctggtttt gcgcaccacc
    45601 atctcggtga actgcacgac cacagcaatc accagaataa atgccagggt gcgtaggtaa
    45661 atcagattaa gtgggatcaa aatccacgta tcgataagcc aggcgcaaat agacgtcagc
    45721 gtcatcacaa acgttgttgc cagccccatg cccatcgcgg tttccagttt tttggaaacc
    45781 cccataaacg gacagagacc gagaaacttg accagtacaa agttattgac cagtacagtt
    45841 ccgacaaaca gtaacaggta gtcagtcatt tttcggcctg aaataaaaaa gccgcctatt
    45901 atccgttaat ccagagcagg cgacaacagg ttaactgtaa ggttattacg gattcacaaa
    45961 cgtcgctttt acccgcgacg agcgtttgaa ataggggacg atcaatgcgg ttgccaacaa
    46021 agtaaacagc aactggcgaa cggctatgcc aacttccacg ggtgaaaaag cgaatgcttt
    46081 tactgccagc aatacggaaa taagcagcca gataatatag tgtttaggaa cacaacgccg
    46141 acgtttaaag aaagcgatgg tcaaccacag tgtgtaatac cacatggcga tagcggtaat
    46201 aaaagaaacg ccccataaga tttgtgtcgt cagtgcttgt ccgccgagcg tttgaaatgt
    46261 ttgcggagaa gataacgcag cggtatacag caacaacgcc agcgtcgtac tcaacagagc
    46321 gactaaaagc caggccaacg ggccaagcaa ccagcctcca atacgttgtg gcgttgtggt
    46381 ggtcataggt tctccagaat agcgacgcaa acaggcaaat ctaattttag gggcgttatt
    46441 ataaggcttt tataaccgat cgctactttc tttattggac atagtgccag acggacttcg
    46501 gtacctggcc gaggtcaaac aaacgcccgc cagaaaccag ctctgcgcga cggtgatcgg
    46561 cagcacgata catattgatc agttcctgat cgtccgtcag ggtgtaatta agatggtcgt
    46621 agagtttttc cagactttcg agtgaactga ttttgcgaaa ttttaataag tagtcctgaa
    46681 cagtcataaa aggtccataa aataattaat accaaagggg taggtattat tagtgatgat
    46741 aaaatttcac caaaagtgaa cgcttgtgtt attacaataa taagaatgcg ctgatggcaa
    46801 atattcaggg atgaaaaact gctctgaaaa gagtaacgcg ctttactatc cctgacaatc
    46861 accaacaaca tcgaacaaga taataaattc ctggtttaat atccgacaag tgaaaacatg
    46921 cacccggacg ggcagcatgt cgctccacaa gtgcagagct tacttgtgtt gtaccgaagc
    46981 actctgttca ggtggctgat agttgtcaat gtgactcgcc acgccaagaa gaatgactga
    47041 aacgacaaga acgatccaac ctgttaattc aataagacga ttcattacag cccacatctc
    47101 tcttgattga tccattaact tcagggggta aatgttactt agcaataata gctccagcaa
    47161 gatttttact gaggtttttg cgatatttag cttttgtcgt tggaaaattc gctatttttt
    47221 tgacttaagt taaacaacat cccttattgc tggcaggtta ttaaactgtt gagcgtgggt
    47281 aaaggatagt gtcaaatagc catcatactt caatgagagg caatgacatg agcgacaaca
    47341 tccgtgttgg gttgattggg tatggttatg cgagcaaaac cttccatgcg cccctgattg
    47401 cgggcacgcc cgggctggaa ctggcggtaa tctccagcag tgatgaaaca aaagtaaaag
    47461 ccgactggcc aacggttacg gttgtctctg agccgaagca tctgtttaac gatcccaaca
    47521 tagacctgat tgtcattcct acacccaacg atacccattt cccgttagcc aaagcggcgc
    47581 ttgaggcggg taaacatgtg gtcgttgata aaccctttac cgtgacactg tcacaagcgc
    47641 gagagctgga tgcgctggca aaaagcctgg ggcgtgtgct gtctgtattc cataaccgtc
    47701 gttgggatag cgatttcttg acgctaaaag gtttgctcgc ggaaggcgtg ctgggtgaag
    47761 ttgcttactt tgagtctcat tttgaccgct tccgtccgca ggtgcgcgat cgttggcgtg
    47821 aacagggcgg cccaggcagc ggtatctggt acgatttagc accgcatctt cttgatcagg
    47881 ccattacgct atttggttta ccggtcagca tgacggtaga tttggcacag ttacggcccg
    47941 gagcgcagtc gaccgattat ttccacgcca tcttgtccta tccacagcgg cgagtcattt
    48001 tacacggtac catgctggca gccgctgagt cagcacggta tatcgtgcat ggatcccgag
    48061 gcagttatgt gaaatatggc ctcgatccac aggaagaacg tctgaaaaat ggcgagcgtc
    48121 tgccgcagga agactggggc tacgatatgc gtgatggcgt acttacccgc gtggaaggtg
    48181 aggaacgtgt cgaagaaacg ctgttgacgg tgcctgggaa ttatccggct tactatgcgg
    48241 cgattcgtga tgcgttaaat ggcgatggtg aaaatccggt tccggcaagc caggcaatcc
    48301 aggtaatgga gttgattgag ctgggcatcg aatccgccaa acatcgcgcg acgttgtgcc
    48361 ttgcgtgaaa aatggatggt gcaatttgca ccatccattt ttgttacttc gcggcgactt
    48421 tttctcgcag tgcgcgtttt tcctctgcgc tgaggaaagc catttccaga ccattaatct
    48481 gtgcctggcg gatttgctca cgggataacc cagcagctgg cgcggcaacg gtatattcgt
    48541 gaatgatatc cactccctgt acgccgggat cgtcagtgtt aatgctggca cgaatgccat
    48601 gctcaaggaa cgttttcagc ggatgtgcag ccagctctgc tacggtgctg gtctgaatat
    48661 tggaggtcag acaggattca ataccaattt gttgctcggc gagaaaatcc atcagcgccc
    48721 gatcttcaat ggcttttacg ccatgtccaa tacgctccgc acccagttca cgaatcgcct
    48781 gccagatgct ttccggcccg gcagcttcgc ctgcatggac ggtaatatgc cagcccgcat
    48841 cacgcgcgcg gttgaagtga gaaaggaaca gacttcccgg gaatccaagt tcatcaccag
    48901 ctaaatcaag tgcggtaatc tggtcacggt gggctaaaaa ggcctccagc tcttgctgac
    48961 aggcggcttc gccgaaggtt cggctcataa tgccgataag cttcgcctgc acaccaaagg
    49021 tgcggcaacc ttcacgtacg ccatcgatca ccgcttcgac aacacccgct acaggcagct
    49081 tatgtgccat tgccatgtag cctggtgaaa aacgcagctc gacatagcgc aggccgttac
    49141 gggctgcatc ttcaatgttt tcaaatgcca cgcggcggca ggcatcaaga gaggcgagaa
    49201 ctttaacgcc ccagtcaagt ttggtcagaa agctcaccag atcgggttcg ttggcaatga
    49261 cctgaacgtg gggaatcagt gtttcaaggg attgtgcagg aagcgagata ttatactggc
    49321 ggccaagttc aagaatggtc tggggacgaa tgttgccatc aaggtggcga tggatatcag
    49381 ttaatggcag ggtggtatca atcatggtcg cactcttttt ctggatataa aagtgcgcac
    49441 cattataaaa acaaagcagg gtaaaaatcc acgattgcgc aacgattttt acgttgcgca
    49501 attggggtta gcgaacagcg cggatggcgt ttattaatcc agccacacct ttttccagtt
    49561 tcgaacgtgg gcagccggca ttgagacgga caaaaccacg accttcttca ccataggtat
    49621 accccggcat gatcgcgact ttttcttgct cgataagcgc tttttgcaac gcgttgtcgt
    49681 caatattcaa cggacgtaga tcaagccagg ccagataagt ggattgcggg atctgccagt
    49741 tgagttcagg aaacgcggcg ttcattttat ctgagatata cgtcaggtta tctttcagat
    49801 agacgcgtaa ggcatccagc cacggtgcac cttgctgata ggcggcgata tgggcagtta
    49861 acgccagtac cgaaggggaa gaaagcccat cacgtccttt cagtgccgat aaataggcat
    49921 cacggctgct gctattttct ataatcccgt aagcaccggt cagcgcggga atattgaaac
    49981 ttttcgatcc cgacgtcagc aacgcccagt ctccccgcgc cacattactc cagggaatat
    50041 gcggctgctc gccccagacc atatccatat ggatttcatc ggaaataacc cgcacatcat
    50101 gacgctcgca caggtcagcc atgatctcca gctcatcgca cgtccacact ttcccggtag
    50161 gattctgtgg gttacacagg agcataattt tacattctgg tttcgccaaa acggcttcca
    50221 gcttgcccat atcgcaaaac cagccgtcag cctgcttctc taaagcaacg ggcattactg
    50281 tgcgctggtt accttcaatg gccttgtaaa atgcgtcata ggcgggcgtg tggatcacca
    50341 cgccttcacc tgtttcagac cactgacgaa tcagttctga aaccatatag atgacagaag
    50401 ggccatacac caccgtctgc gtatcaattg cggtgtaatg ctgggtggaa aaccagtggg
    50461 caatagccgc gagaaactca tcgtttttcc agcggctgta gccaaatacg ccgtgcatca
    50521 ggcgctgatt cagcgcctcg ataatgcagg gggcagtggc aaaatccatg tctgaaatcg
    50581 tgaacggtaa caggtcagca gtgccgaaac ggtcagcgac ataatcccac tgtgtacacc
    50641 atgtgccatg acgatccacg acctttgaaa aatcgaacat atcttatcct tatgcctgga
    50701 cagtatgcat cagaccggcc atttcatctt ttactgactg aacttgtggc ccgataacaa
    50761 cctgcaggtt atgttgatta agttgtacta cgccaattgc ccgattgtcc ttcagtgcct
    50821 gcacattaac aagcgacata tcttccacag acaaacgcag acgggtaatg cagttatcga
    50881 ggctgacaat attgtcggca ccgcctaatg cttcgaggat tgcaggaacg ttgtaacctg
    50941 atttacccgg cgcaccggca acggcttttt cgattgagct ggcaacttcg ctatcgcgcc
    51001 ccggggtttt cagattgaag cgggtgatag cgaaacggaa gatgacgtag taaacgacaa
    51061 accagattgc cgccaccact ggcaccatgt accacttggt tgacagacca tgcaaaatac
    51121 cgaacaccac gaagtcgatg atattgccgt cggtattacc gatggtgacg ccgagcacag
    51181 acatgacggt gaagccgagg ccggttaaca gcgcgtggat gacatacaga actggcgcta
    51241 cgaacaggaa caggaattcc agcggttcgg tagtgccgcc aacgacgcag gcgatcaggc
    51301 cagaaatcag cagaccttta attttatggc gattttccgg gcgcgcgcag tgatacatag
    51361 ctaacgctgc acctggcaga ccgccgagaa acgcaggcat tttaccttgc gaaaggaaac
    51421 gcgtgacgct ttcagaaaaa ccgtgagtgg tcgggcaact caattgcgcc tggaagatgg
    51481 tcagtgcgcc gctgacggtt tgaccgcaga cctcctgcgt gccgcctgcg tcggtaaagc
    51541 gaattaatgc caccagaatg tgatgcagac caaacggtaa cagcagacgt tcaccggtac
    51601 caaacagcat cggtccgaaa tcacccgcgc tgtttatcat atggcccaag ccgctaatac
    51661 ccatggcgaa aatcggccag actaatggaa tcaccaggcc gacaaggccc atcaccagcg
    51721 aggagataat tggtacgaag cgcgtaccgc cgaagaatgc cagcgcatcc ggcaggcgga
    51781 tattatggaa acgctcatgc agcatccaga cgataatacc ggcgatcacc gcaccgagga
    51841 tcccggtatc gatcgactgg atcccaagaa tgctctggat gttattggct ttcagaacgg
    51901 cggcatctgt ggttggcaga atgcctttat tggtcaacca gaagtttacc gcgaggttca
    51961 ttaccgcata accgacgaag ccagcgaatg ccgctacgcc tttattttcg cgtgccaggc
    52021 ccagcgggat ggcgatacag aacatgacag gcaggaaact aaaagcaaac gagccaatct
    52081 tactcatcca ggtaaagata gcctgcaaca cggggttgcc caggaccggg atcagggtta
    52141 tgacatcatg gctgctaaga gaactaccaa tgccgagcat aatgccgcag aacgacaata
    52201 atgccacggg taacatgaag gttttgccta actgctggaa gaactcccac agcgtgactt
    52261 tcggtgctgt tttcgccgtc ataaaacgac tcctcgtaga gaacaatctg gattcagtaa
    52321 attgcgcgag aagataaaac gttttatcaa attttagtga gtcataaatc acattacgca
    52381 acgataatag cgggtataag ataaataaaa ggtaaaacgt tttatctgtc acataatcag
    52441 ggagtaggtc atctgcatgg ctgccgccaa aaaaataacc attcatgatg ttgcgctggc
    52501 tgcgggcgtg tcggtaagta ccgtttcgct ggtgcttagt ggcaaagggc gaatctctac
    52561 cgccacagga gaacgcgtta acgccgccat tgaagagctg ggatttgtgc gcaatcgcca
    52621 ggcgtcggcg ctgcgcggcg ggcaaagcgg cgtcattggt ttgatcgtcc gtgatttatc
    52681 tgcgccgttt tacgccgaat tgacggccgg attgacggaa gctctggaag cgcagggacg
    52741 gatggttttt ttgcttcacg gcggtaaaga cggcgagcag ctggcacagc ggttttcact
    52801 gttactgaat cagggtgtcg atggtgtggt aattgccggg gctgcaggaa gcagcgatga
    52861 cctgcggcgg atggcagaag aaaaagctat cccggtgatt ttcgcttccc gtgccagtta
    52921 tcttgatgat gttgatacgg ttcgcccgga caacatgcag gctgcacagt tgttgacgga
    52981 gcatctcatt cgcaatgggc atcagcggat cgcctggctg ggagggcaaa gttcctcatt
    53041 aacccgtgca gaacgggtgg ggggctattg tgcaactcta ctaaaatttg gcctgccgtt
    53101 tcacagcgat tgggtgttgg agtgcacttc cagccagaag caagccgcgg aagctatcac
    53161 ggcgctttta cgtcataacc cgaccatcag tgccgtggtt tgctataacg aaactattgc
    53221 gatgggggca tggtttggtt tgctgaaagc agggcggcaa agcggggaaa gcggagtcga
    53281 tcgttacttt gagcaacagg tttcgctggc ggcatttacc gatgcgacac caaccacact
    53341 tgatgatata cctgttacct gggccagcac gccagcgcgg gaacttggta tcacacttgc
    53401 ggatcgcatg atgcaaaaaa tcacccatga agagacgcat tcacgcaatc ttattattcc
    53461 cgcccggctc attgcggcga aataatcctc tctttatctg ctatacttgg tagtgtcctt
    53521 tcctcaaggt taatgataaa aaatgtggca cacaggcggt gccttttatt tttactgaag
    53581 ttcacaggag gtttatgtgt ttaattctga caacctgaga ctcgacggaa aatgcgccat
    53641 catcacaggt gcgggtgcag gtattggtaa agaaatcgcc attacattcg cgacagctgg
    53701 cgcatctgtg gtggtcagtg atattaacgc cgacgcagct aaccatgttg tagacgaaat
    53761 tcaacaactg ggtggtcagg catttgcctg gcgttgtgat attacttccg aacaggaact
    53821 ctctgcactg gcagactttg ccgtcagtaa actgggtaaa gttgatatcc tggttaacaa
    53881 cgccggtggc ggtggtccta aaccgtttga tatgccaatg gcagattttc gccgtgctta
    53941 tgaactgaat gtgttttctt ttttccatct gtcacaactt gttgcgccag aaatggaaaa
    54001 aaatggcggt ggcgttattc tgaccatcac ttctatggcg gcagaaaata aaaatataaa
    54061 catgacttcc tatgcatcat ctaaagctgc ggccagtcat ctggtcagaa atatggcatt
    54121 tgacctgggt gaaaaaaata ttcgggtgaa tggcattgcg ccgggggcaa tattaaccga
    54181 tgccctgaaa tccgttatta cacca
//
LOCUS       DAETPZ010000021        53212 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.021, whole genome shotgun sequence.
ACCESSION   DAETPZ010000021 DAETPZ010000000
VERSION     DAETPZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.021"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(12..323)
                     /locus_tag="K0W59_001380"
     CDS             complement(12..323)
                     /locus_tag="K0W59_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HBI3022483.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            486..582
                     /gene="ffs"
                     /locus_tag="K0W59_001381"
     ncRNA           486..582
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="K0W59_001381"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            702..1055
                     /locus_tag="K0W59_001382"
     CDS             702..1055
                     /locus_tag="K0W59_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HBI3022484.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1097..2647)
                     /gene="pdeB"
                     /locus_tag="K0W59_001383"
     CDS             complement(1097..2647)
                     /gene="pdeB"
                     /locus_tag="K0W59_001383"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HBI3022485.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FINVIPYSSWQIDAAIIGNAHNVVITSSDKIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2811..3281)
                     /locus_tag="K0W59_001384"
     CDS             complement(2811..3281)
                     /locus_tag="K0W59_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HBI3022486.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3397..3948)
                     /gene="maa"
                     /locus_tag="K0W59_001385"
     CDS             complement(3397..3948)
                     /gene="maa"
                     /locus_tag="K0W59_001385"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HBI3022487.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4120..4338)
                     /gene="hha"
                     /locus_tag="K0W59_001386"
     CDS             complement(4120..4338)
                     /gene="hha"
                     /locus_tag="K0W59_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBI3022488.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4364..4738)
                     /gene="tomB"
                     /locus_tag="K0W59_001387"
     CDS             complement(4364..4738)
                     /gene="tomB"
                     /locus_tag="K0W59_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HBI3022489.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5284..8433)
                     /locus_tag="K0W59_001388"
     CDS             complement(5284..8433)
                     /locus_tag="K0W59_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBI3022490.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAVSLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8456..9649)
                     /gene="acrA"
                     /locus_tag="K0W59_001389"
     CDS             complement(8456..9649)
                     /gene="acrA"
                     /locus_tag="K0W59_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HBI3022491.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9791..10438
                     /gene="acrR"
                     /locus_tag="K0W59_001390"
     CDS             9791..10438
                     /gene="acrR"
                     /locus_tag="K0W59_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HBI3022492.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10566..13928
                     /gene="mscK"
                     /locus_tag="K0W59_001391"
     CDS             10566..13928
                     /gene="mscK"
                     /locus_tag="K0W59_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HBI3022493.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISGLLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14140..14301)
                     /gene="rsmS"
                     /locus_tag="K0W59_001392"
     CDS             complement(14140..14301)
                     /gene="rsmS"
                     /locus_tag="K0W59_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HBI3022494.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14315..14842)
                     /gene="priC"
                     /locus_tag="K0W59_001393"
     CDS             complement(14315..14842)
                     /gene="priC"
                     /locus_tag="K0W59_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HBI3022495.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREAAAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14912..15289
                     /locus_tag="K0W59_001394"
     CDS             14912..15289
                     /locus_tag="K0W59_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HBI3022496.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15442..15993
                     /gene="apt"
                     /locus_tag="K0W59_001395"
     CDS             15442..15993
                     /gene="apt"
                     /locus_tag="K0W59_001395"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBI3022497.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16122..18047
                     /gene="dnaX"
                     /locus_tag="K0W59_001396"
     CDS             16122..18047
                     /gene="dnaX"
                     /locus_tag="K0W59_001396"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBI3022498.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWTAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17380..17444
                     /gene="dnaX"
                     /locus_tag="K0W59_001396"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18100..18429
                     /locus_tag="K0W59_001397"
     CDS             18100..18429
                     /locus_tag="K0W59_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBI3022499.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18429..19034
                     /gene="recR"
                     /locus_tag="K0W59_001398"
     CDS             18429..19034
                     /gene="recR"
                     /locus_tag="K0W59_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBI3022500.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19144..21018
                     /gene="htpG"
                     /locus_tag="K0W59_001399"
     CDS             19144..21018
                     /gene="htpG"
                     /locus_tag="K0W59_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBI3022501.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21199..21843
                     /gene="adk"
                     /locus_tag="K0W59_001400"
     CDS             21199..21843
                     /gene="adk"
                     /locus_tag="K0W59_001400"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBI3022502.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            21975..22937
                     /gene="hemH"
                     /locus_tag="K0W59_001401"
     CDS             21975..22937
                     /gene="hemH"
                     /locus_tag="K0W59_001401"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HBI3022503.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22934..23894)
                     /gene="aes"
                     /locus_tag="K0W59_001402"
                     /pseudo
     CDS             complement(22934..23894)
                     /gene="aes"
                     /locus_tag="K0W59_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24046..25350
                     /gene="gsk"
                     /locus_tag="K0W59_001403"
     CDS             24046..25350
                     /gene="gsk"
                     /locus_tag="K0W59_001403"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HBI3022504.1"
                     /translation="MKFPGKRKSKNYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(25483..27159)
                     /locus_tag="K0W59_001404"
     CDS             complement(25483..27159)
                     /locus_tag="K0W59_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HBI3022505.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27397..28617)
                     /gene="fsr"
                     /locus_tag="K0W59_001405"
     CDS             complement(27397..28617)
                     /gene="fsr"
                     /locus_tag="K0W59_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HBI3022506.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALPGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMISGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28835..30487
                     /gene="ushA"
                     /locus_tag="K0W59_001406"
     CDS             28835..30487
                     /gene="ushA"
                     /locus_tag="K0W59_001406"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBI3022507.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30524..31003)
                     /gene="ybaK"
                     /locus_tag="K0W59_001407"
     CDS             complement(30524..31003)
                     /gene="ybaK"
                     /locus_tag="K0W59_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HBI3022508.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31207..32001)
                     /locus_tag="K0W59_001408"
     CDS             complement(31207..32001)
                     /locus_tag="K0W59_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HBI3022509.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALLVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32139..32480
                     /locus_tag="K0W59_001409"
     CDS             32139..32480
                     /locus_tag="K0W59_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBI3022510.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32695..35199)
                     /gene="copA"
                     /locus_tag="K0W59_001410"
     CDS             complement(32695..35199)
                     /gene="copA"
                     /locus_tag="K0W59_001410"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HBI3022511.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAAFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35461..36393
                     /gene="glsA"
                     /locus_tag="K0W59_001411"
     CDS             35461..36393
                     /gene="glsA"
                     /locus_tag="K0W59_001411"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBI3022512.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVLGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36396..37688
                     /locus_tag="K0W59_001412"
     CDS             36396..37688
                     /locus_tag="K0W59_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI3022513.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37813..38220
                     /gene="cueR"
                     /locus_tag="K0W59_001413"
     CDS             37813..38220
                     /gene="cueR"
                     /locus_tag="K0W59_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HBI3022514.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38221..38679)
                     /locus_tag="K0W59_001414"
     CDS             complement(38221..38679)
                     /locus_tag="K0W59_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBI3022515.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTYVEVIAIEGITLHIRAVSS"
     gene            complement(38676..39593)
                     /locus_tag="K0W59_001415"
     CDS             complement(38676..39593)
                     /locus_tag="K0W59_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBI3022516.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39739..40416
                     /gene="fetA"
                     /locus_tag="K0W59_001416"
     CDS             39739..40416
                     /gene="fetA"
                     /locus_tag="K0W59_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HBI3022517.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EVRYELA"
     gene            40403..41182
                     /gene="fetB"
                     /locus_tag="K0W59_001417"
     CDS             40403..41182
                     /gene="fetB"
                     /locus_tag="K0W59_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HBI3022518.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41245..42099)
                     /gene="cnoX"
                     /locus_tag="K0W59_001418"
     CDS             complement(41245..42099)
                     /gene="cnoX"
                     /locus_tag="K0W59_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HBI3022519.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42160..42969)
                     /gene="ybbO"
                     /locus_tag="K0W59_001419"
     CDS             complement(42160..42969)
                     /gene="ybbO"
                     /locus_tag="K0W59_001419"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBI3022520.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42959..43581)
                     /gene="tesA"
                     /locus_tag="K0W59_001420"
                     /pseudo
     CDS             complement(42959..43581)
                     /gene="tesA"
                     /locus_tag="K0W59_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43552..44238
                     /locus_tag="K0W59_001421"
     CDS             43552..44238
                     /locus_tag="K0W59_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI3022521.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44235..46649
                     /locus_tag="K0W59_001422"
     CDS             44235..46649
                     /locus_tag="K0W59_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3022522.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTDDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFVG"
     gene            complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="K0W59_001423"
     CDS             complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="K0W59_001423"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBI3022523.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48524..49450)
                     /gene="allS"
                     /locus_tag="K0W59_001424"
     CDS             complement(48524..49450)
                     /gene="allS"
                     /locus_tag="K0W59_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HBI3022524.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQHRPEISGFLEIFGNPRS"
     gene            49680..50162
                     /gene="allA"
                     /locus_tag="K0W59_001425"
     CDS             49680..50162
                     /gene="allA"
                     /locus_tag="K0W59_001425"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HBI3022525.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50240..51055
                     /gene="allR"
                     /locus_tag="K0W59_001426"
     CDS             50240..51055
                     /gene="allR"
                     /locus_tag="K0W59_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HBI3022526.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51145..52926
                     /gene="gcl"
                     /locus_tag="K0W59_001427"
     CDS             51145..52926
                     /gene="gcl"
                     /locus_tag="K0W59_001427"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HBI3022527.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52939..>53212
                     /locus_tag="K0W59_001428"
     CDS             52939..>53212
                     /locus_tag="K0W59_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HBI3022528.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12818 a        13705 c        14023 g        12666 t
ORIGIN      
        1 cccggagagg gttagtagtt ccagcgataa cgctgcaagt cgatttgccc gcttcccgat
       61 accatcacac cttctgccag taatgcctgt cgctgacgct gtaaatccgg cccggttagc
      121 gaaattgtgc catggcgatt aaccacccgg tgccagggta aggtgctgcc ttcagggaga
      181 cgctttaaca caccgcccac ctggcgcgcg gcgcggggcg atcccgccag tttcgccaca
      241 tcaccgtaag tggtgacata gccttcggga atagcggcga cgatttgcca gacgcgttgg
      301 ggaaatgaat cttctttttc catcttttct tcctgaggta atttttcagc ataatctgga
      361 aaaacgcccg agtgaagtcg cattgcgcaa gaaaccagca tctggcacgc aatgggttgc
      421 aattagccgg ggcagcagtg ataatgcgcc tgcgcgttgg ttttcaacgc tctcaatggg
      481 ggctctgttg gttctcccgc aacgctactc tgtttaccag gtcaggtccg gaaggaagca
      541 gccaaggcag atgacgcgtg tgccgggatg tagctggcag ggcccccacc catttctgcc
      601 tcccaccgtt tcgtcaaaaa atcccagcat ggctaaactt taaccacaac tgacgtcgca
      661 agaattgtct ggctgcgcag tacgcttcgg aggtatgtct gatgaagtat gttgatggtt
      721 ttgtggttgc cgttcctgcc gataaaaagg atgcctatcg ggaaatggcc gctaaggccg
      781 cgccattgtt taaagagttt ggcgcgcttc gtattgtcga atgctgggcc agcgatgtac
      841 cggatggcaa agtgaccgat tttcgtatgg cggtgaaagc ggaagagaat gaagaggttg
      901 tctttagctg gattgaatac ccttcaaaag aggtccgcga cgcagctaat caaaagatga
      961 tgtcggaccc acggatgaaa gagttcggcg agtccatgcc gtttgacggc aagcgaatga
     1021 tctatggcgg attcgagtca atcatcgacg aataggtagc gtgacgggct gtgcggcgtg
     1081 ccgtcagccc aaaaattcac aaatgttgct cggcccagcg taagaaatct tctttcggta
     1141 atgccttgct gtagagccag ccctgaccgt agtgcacgcc atgctggcgt agccactctt
     1201 cttgtttact ggtttcgatt ccctccgcta ccattttcag tttcagtgtt tttgccattt
     1261 cgatgatatg cggcgtgaca tttttatatt ccagcgcatc aacgaaagat ttatcgatct
     1321 tcagaatgtc gacatccaga tcctgtaaat aacttaaact tgaatacccc gtaccaaaat
     1381 catcaagata aatttcatgg cccgcctccc ggtagcgaga aattatcggg gcgctggttt
     1441 tcggatcggc aaactcgcgt tcagtgagtt caagcgcgat ctgtctggga ttaacctgat
     1501 agtgattgat catttcacgc agcaattgcg ggattttttc cgaggtgagc acggtggatt
     1561 caagattgat cgaaatatgc tgctgtggat gctgacgcaa gcagtcgccc atatcttcaa
     1621 agacgcttct tataatcagt agcgtcaatg gctcagaaag gcccgtttgc tgtgccagcg
     1681 gaataaaact atctggtgac aaccaactac cgtctgtctg cggccagcgc gccagtgcct
     1741 cagcaccgac aattttgcca ttggctaagg agacaatcgg ctgatagtgc acgcaaatat
     1801 cacgattttc gatagcatcc tgcagccgat gatgcggtga ctggatacgg cgcaaaatac
     1861 gcagcacaaa catcgctgcc agcaggccaa tcaccaaccc gagcggtaac caaataaaga
     1921 cttgccgatg ccagcctttc tgcaacattt tcgttgaagc ccaggtgatg atcgaaatat
     1981 tcatctccgg gaagggcagg atatcgtaaa tgatcccatt attttcgata tgctcaccgg
     2041 gtgttttttg tagcctggta ataattccct gagcaatttt atcgctgctg gttatgacaa
     2101 cgttatgggc attgccaata atggcggcat caatttgcca tgagctatag ggaatgacat
     2161 taataaagga agcggggtcg atcatgacga cataatgtgc cgttcccatg gcgaccatgt
     2221 aacggataat gcctaaatcg ttatgcgatg ttaaccagac acgataacca tctttcgaaa
     2281 ttttacctgg ctcgggaaag gtatcgggcg gactttcatg ctccagagac gaacactggg
     2341 gaacgttgtt atcgatataa accacttcct gaatatagcg ataactgtaa gatacccgac
     2401 gcatttccat gagatgggct tcgctacagg cagcgccttg ccatctttcc agctcctgca
     2461 gcgcatcttt cccttgtgtc gccaccttat tggctcgaat agcgacgcgg gaggaatagg
     2521 tatccagctc ttcaataaac gatgtttcta cctgctgatg ggccagccag atgcttaagc
     2581 cgacaggcag caatactgaa agaatcagta ctcccgaaat aaggccgacc agatgtcgtg
     2641 ttctcaccag catatcctta ttaacacagt gtagggaaag tatatctgat tgtggaaaaa
     2701 ggctggcagg agataaggag gtagagctgg tatttcatga agtgatgggt ggaaacgatc
     2761 attgtcattt gaccaggcgc agagctttac gcctggtatg taaccctaac ttaagatgtt
     2821 gattcggcgc gagcgagggt aaaaatatcg taaaaagaca gttcaccttt acggacgatc
     2881 attttttgca gttgttcctt atgaacatcc gcgacacgcg gagagttcaa gatggcttca
     2941 accagcgcgg caggtacaaa gcgcgtgttg taccattcgc cgttatagca aacgcggaaa
     3001 tccagcacgt cgatatcttc atagcggtag cgtgggtaga catcgaaaaa gaagaaacca
     3061 ttaagcagga taaataatac aaccagtagc cagacagagc ctgacagcgt ttcggactgc
     3121 aacatcaccg ccagggtggc aaaaaaagca acgtacatac cgataaacag ccccggatgt
     3181 ttacggataa aactgatact aaagcggggt ttgttgtcgc gtttttcgcg ggtattcaga
     3241 gactcaatcg tttcggtcag caggcgttgt atttcggtca tcgtttgcct tcgtggtatc
     3301 tgcaactttc cagggaacac cctattttaa cgtgggaata gtagagggga agtaacagat
     3361 ctaccacaat tttgcataac agttgcgaaa aaccgattac aattttttaa ttattctggc
     3421 tggattaccg cccacgacaa cgttgtccgg gacacctttt gtgacaactg cacctgaagc
     3481 taccacgacg ttatcaccaa tggtcacacc agggttaatg accgcgcgtc cgccaatcca
     3541 gacgttatta ccgatggtga cgggtttccc cagttcagca ccgctattac gtgctacagg
     3601 gtcgatggga tgtgttgccg tgtagatatg aacgcctggt gccaacatac agttatcacc
     3661 gatgcgaata gggcagacat caagcatcac gcaatcgaag ttggcgaaaa aattattgcc
     3721 gagaaaaatg ttatagccat aatcacagcg aaacgttggc tcaatataag cctctgtcac
     3781 ctgaccgaat aaatcagcga gaatttgctg gcgtaatgtg tgctcttccg ctagggaatg
     3841 attgtatcgg tgaataagct gacgagcgcg caggcgatcg cgagataacg tctcatctgc
     3901 cgagcgatac aactcaccag caatcatctt ttctttttct gtgctcatta ctgacctccg
     3961 gttgctgaaa atgcggcaac acagtaagac gaaaagtcag tgaagagaac gttctcatta
     4021 catctgtgtg ataaagatca catagggtat gtcttcatgg cgaaaaagta taaaattctt
     4081 aataaacagc cggttatagc tccgaaagcg aattatttat tagcgaataa atttccatac
     4141 tgaggaaggg atcttgtcgt acagtttatt catggtcaat tcggcgaggc ggtgatctgc
     4201 ggctgagtaa aataccgcca gttcattatc tgataattcg tatttatttt tctcgataac
     4261 acgctccagc gtgtcaattg tctggcaacg acgtaaacgc attaaataat cggtttttgt
     4321 taaaggtttt tcggacatac ttctacccat ggttgtaata attctaacaa gataaactcg
     4381 caggattctc tttcgtcgca ttgacaaaac aacggaatag tcgattacct gacttccgcc
     4441 atttctgaag agcctgcata ttaataccat aactgctgaa caacataaag gtgtcatcca
     4501 gatattcgtc gatctgctca atgagcttat tgtcttcatt atacttaatt ttgtaattaa
     4561 gtgcgaaggt cgcaatatgc tcaatcagtt cattcaactg gaggttgatc gccgaggttg
     4621 ggtcgttaac ccagccatga ttgctttctt caaggtttgc aagacagtca tgatacaggg
     4681 tttcacagag aaacttaagc tgtgcgatat catgtctttt gggtgagtat tcatccataa
     4741 cgcgtcccct tcttagtggt tgaactaacg gacacctttc gggatggaaa aaacttactg
     4801 acctagactt gccctctcgt ttgttagctt aactataagc cactctttgc aggttttcat
     4861 cgcattatta cgaaacatta caattaggaa ggaatcggga caggactcac tttttcatac
     4921 tagcactaac ccgcagcagg taaaagcagt ttgctcgaac tgttcataac atttcaggat
     4981 tatcgccaga tgaaattggt gacactaata ccaggattgc tctgaatatg acgtaataac
     5041 cgaggaatga ataaagaatt accgcaaata attaagaata gcctctaaat gattatggat
     5101 tattaaatct attagcgcta cttaatataa attaactaac aatcagtatt ttttatgaat
     5161 tttatacgtg gttaatactg gttttcgtat gagatcctga gttggtggtt caattactcc
     5221 ttaatgttcg taggttatgc ataaaaaagg ccgcttacgc ggccttagtg attacatgtt
     5281 gtatcaatga tgatcgacag tatggctgtg ctcgatatct tcattcttgc ggctaaagcg
     5341 gcggcgaacc accacaaaga ataccggaac gaagaagatt gccagtaccg ttgcggtcac
     5401 catcccgccc attacaccgg tacctactgc gttctgcgcg ccggaaccag caccagtact
     5461 gataaccagc ggcataacgc cgaggataaa cgccagcgag gtcatcagga tcggacgtaa
     5521 acgcatccgc accgcatcaa gcgtcgcttc aatcagacct ttaccttctt tatccatcaa
     5581 gtctttggcg aattcgacga taagtatcgc gttcttcgcc gacaacccaa tggttgtgag
     5641 cagtcctact tggaagtaaa cgtcattggt caggccacgg aaggtggcag ccagcaacgc
     5701 accgataacc cccagcggaa cgaccagcat aacggagaac ggagtcgacc agctctcgta
     5761 cagcgccgcc agacacagga atacgacaat caacgaaacc gcgtacagtg aaggtgcctg
     5821 gttgccggag agacgttcct gataggacat ccccgtccag tcatagccaa caccggtagg
     5881 cagtttgctc gccagttgtt ccatcagctc cattgcttca ccggtacttt tacccggtgc
     5941 cgcctggcct aagatttcca tggatggcag gccgttgtaa cgttccagac gcggcgaacc
     6001 gtactcccaa cgagaagagg agaacgccga gaatggcacc atctgaccat cagcagcacg
     6061 aacataccag tcgccgatat catccggcag catacggtat ttcgcttctg acatgacgta
     6121 aactttcttc acacgaccgc ggtcgataaa gtcgttcaca tagctaccgc cccatgcagc
     6181 gcccagagtg gtgttaatgt cgttgataga aacacccagc gcctgcgctt tttcctggtc
     6241 gatatcaatc ttaaactgcg gggtatcttc cagaccgttt ggacgtacgc tggtcagcat
     6301 atcagggtgc ttcgctgctt ctgcaagcag ctggttacgc gcctgagtca gtttttcgtg
     6361 accaaggcca gcctggtcaa tcagctcaaa gtcaaagccg gttgcagtac ccagttccac
     6421 gattgcgggc aggttaaagg cgaaaaccat cgcatctttg atttgcgaga aagcgcgtgt
     6481 tgcacgcatg gtaatcgctt caactttgtt ttcttcgccc ggacgatcgg cccagtcctt
     6541 caaggaaacg aacgcaatac cggtattctg accacgtccc gcaaagccga agccgttaac
     6601 ggcgaacacc gactcaacgt tgtttttttc tttggtcaga tagtaatgcg ttacctcatt
     6661 gagcactttc tgcgtacgtt cctgcgttgc acctgctggc agctgaacca tggtcataaa
     6721 tacgccctgg tcctcatctg gcaagaagga gcttggcaga cgcacgaaca gataggccat
     6781 gccgaccacg atgatcagat acagcactag gtaacgcccc gtactgcgca gaataccgcc
     6841 tacgctgtcg gtgtagtggt gcgtgctctt ctcgaacatg cggttaaacc agccgaagaa
     6901 gcctttttta ccttccccgt gatcgccttt ggcaatcggt ttcagcatgg tggcacaaag
     6961 agccggagtc aggatcaacg ctaccagtac cgacagcgcc attgctgaaa caatggtaat
     7021 agagaactga cgatagatag caccagtaga accgccaaag aaggccatcg gtacgaatac
     7081 cgccgacagt accatcgcga taccgaccag agcgccctga atctgcccca tcgacttacg
     7141 ggtagcttct tttggcggca aaccttcttc cgccataaca cgctccacgt tttctaccac
     7201 aacgatagcg tcatccacca acaagccgat ggcgagcacc atcccgaaca ttgttagcgt
     7261 gtttatcgag aagccaaagg cggcaaggac ggcaaaggtc ccgagcaata ccaccggtac
     7321 ggcaatggtc ggaatcaacg tcgcgcggaa gttctgcagg aacagataca taaccaggaa
     7381 cacgaggatg atcgcttcga ccagcgtttt aaccacttcg tgaatagaga ttttcacgaa
     7441 cggcgtggtg tcgtacgggt aaacaatttt cagacccgac gggaagaacg gttccatctt
     7501 cgccagttca gcacggattg ccgcagcggt atccagcgcg tttgcaccgg tcgccagctt
     7561 gatccccaga ccggaagccg gttggccgtt aaactctgcg atgatgtcgt agttctcacc
     7621 acccagctca atcttcgcca catcacgcag cagtacgcgg gaaccatcct gattcacttt
     7681 cagcaggatt ttgccgaact cttcagtaga ggtcagacgc gtctgagcaa taatagaggc
     7741 gttaagctgt tggcctttca ccggcggcgt accaccgagc tgacccgccg caacctgggc
     7801 gttctgcgct ttgatggcgg taatgacatc aaccggcgtt agctggaatt tgttcagctc
     7861 attcgggttc atccagatac gcatcgcgta ctgtgaaccg aacaactgaa catcacccac
     7921 gcccgacgta cggctgatgg catctttcat attcgccgct acgtagtcag agatatcctc
     7981 ctgcgtcatg gtgccatcgg tgttgataac gccgacaacc atcaggaagc tgctggatga
     8041 tttctcaacg ctcacccctt gctgctgaac ttcttgcggc agcaacggca tcgccagctg
     8101 cagtttgttc tgcacctgaa cctgcgcgat atccgcatca gtaccagact caaaggtcag
     8161 ggtgatctgc acggtacccg tggagtcact gttagaggac atgtacatca ggttatcgat
     8221 accgttcata ttctgttcga taacctgtgt caccgtgtcc tgcactgttt tcgcatcagc
     8281 gccggggtag gaggcggaga tcgttactgc cggcggtgca atcgtaggat attgcgccac
     8341 cggcagtttg aggatcgcca gcccccctgc caacatgatg ataatggcga tcacccacgc
     8401 aaaaatcggg cgatcgataa agaaattagg catgtcttaa cggctcctgt ttaagttaag
     8461 acttggactg ttcaggctga gcaccgcttg cggcttgctg gttattatca gcggtaactt
     8521 cttgtgcttt tacctggaca ccaggacgca ctttctgcag cccacttatt actacgcgat
     8581 cgcctgcttt cagaccttct gtcaccagcc acttatcgcc aatagcctgg cttgcaacga
     8641 tcggacgggt ttccactttg tcatccgcgc caactaccag tacggtggca tcaccacgcg
     8701 gcgtacgggt tacgccctgt tgcgggacta aaatagcgtt tggattaagc ccttcttcca
     8761 gacgtgcgcg cacgaacata cccggcagca gagtgtgatc cgggttcggg aagatagcgc
     8821 gtagggtgat agacccagtg gtctgatcaa cggtaacgtc agagaattcc agcgtaccgt
     8881 cctgcgggaa cttaatgccg tcactggtga tcagcgacac tttggctttg ccgttctctt
     8941 gtttcagcgt gccattcgcc agttcctgtt tcaggcgcag gaagtcgttg ctggactggg
     9001 tcacatcaac gtagatcgga tcaagttgct gcacggttgc cagcgcagtc gcctgaccgt
     9061 tctgtaccaa tgcgccttcc gtcacgttcg acttaccaat gcgaccgcta atcggagagg
     9121 tgactttggt gtaagccaga ttgatccgcg cagtttcaac ggcagctttc gccgcagtta
     9181 ccgcagcatt cgcctgttgc gcatcagcca gagcctgatc gtacccttgc ttactgatgt
     9241 actgagtacc gagcagtttc tgataacgat tcaccgtcaa ttgcgcgata ttggctgcag
     9301 cctgggcttt cgccagatca cctttcgcac tgtcgtatgt cgcctgatag gtcgcaggat
     9361 caatctgata gagagagaca ccagcttcga tgtcgctacc ttctttgaaa ttacgcttca
     9421 ggataatccc gctaacttga ggacgaactt ctgcgatccg gtaggcactg gtgcgacccg
     9481 gaagctcggt tgtgatctgc agaggttcag ttttgactgt tactacgcca acggcgggca
     9541 tctgctggcc accttgttgg gcctgtttgt cgtcacatcc tgttagggct aagctgcctg
     9601 agagcatcag aacgaccgcc agaggcgtaa accctctgtt tttgttcata tgtaaacctc
     9661 gagtgtccga tttcaaattg gtcaatggtc aaaagttaat aaacccattg ctgcgtttat
     9721 attatcgtcg tgctatggta catacattca caaatgtatg taaatctaac gcctgtaaat
     9781 tcacgaacat atggcacgaa aaaccaaaca agaagcgcaa gaaacgcgcc aacacatcct
     9841 cgatgtggct ctacgtcttt tctcacagca gggggtatca tccacctcgc tgggcgagat
     9901 tgcaaaagca gctggcgtta cgcgcggtgc aatctactgg cattttaaag acaagtcgga
     9961 tttgttcagt gagatctggg aactgtcaga atccaatatt ggtgaactag agcttgagta
    10021 tcaggcaaaa ttccctggcg atccactctc agtattaaga gagatattaa ttcatgttct
    10081 tgaatccacg gtgacagaag aacgacgtcg attattgatg gagattatat tccacaaatg
    10141 cgaatttgtc ggagaaatgg ctgttgtgca acaggcacaa cgtaatctct gtctggaaag
    10201 ttatgaccgt atagaacaaa cgttaaaaca ttgtattgaa gcgaaaatgt tgcctgcgga
    10261 tttaatgacg cgtcgggcag caattattat gcgcggctat atttccggcc tgatggaaaa
    10321 ctggctcttt gccccgcaat cttttgatct taaaaaagaa gcccgcgatt acgtcgccat
    10381 cttactggag atgtatctcc tgtgccccac gcttcgtaat cctgctacta acgaataacc
    10441 ctgaatctga ctccaggatt tttcctggac attttcgtcg ttgctattct ggttcactgc
    10501 gtcgtgatat tcttgcggtt tgactttttc aggtcgttct tcaggttcag aaaccttcat
    10561 tcatcatgac tatgttccag tattacaaac gatcacggca ttttgttttt tcagcattta
    10621 ttgcttttgt ttttgtcttg ttatgccaga acacggcgtt tgcgcgggcg tcatcgaatg
    10681 gtgatctgcc gacaaaagcg gacctgcagg cgcaacttga ctcactaaat aaacaaaaag
    10741 atctttctgc tcaggacaaa ctggtgcagc aggatctgac agatacatta gccaccctcg
    10801 ataaaatcga tcgcataaaa gaagagacag ttcagctacg gcaaaaagtc gctgaagcgc
    10861 cggaaaaaat gcgccaggcg accgcggcgt taacagcact tagcgatgtc gataacgacg
    10921 aagaaacgcg caaaattctg agtacgcttt cgttgcgcca gctggaaact cgcgttgccc
    10981 aggcgctgga cgatttgcaa aacgcacaaa acgatctggc gtcttataac agccagctgg
    11041 tttcgttaca gacgcagccc gaacgcgtgc aaaatgcgat gtataacgct tcgcagcagc
    11101 tgcaacaaat tcgcagtcgt ctggatggga ctgatgtcgg cgagactgcc ttacgtccca
    11161 gccagaaagt gttaatgcag gctcagcagg cgttgctgaa tgcggagatt gaccagcagc
    11221 gtaaaagcct ggaagggaac accgtcttgc aggatacctt gcaaaagcaa cgtgattacg
    11281 tgacggcgaa cagcgcccgt ctggagcacc agttgcagct gttgcaagaa gcggtaaaca
    11341 gcaagcgcct gactttaact gaaaaaacgg cgcaggaagc cgtctccccg gatgaagccg
    11401 cgcgtattca ggctaatccg ctggtgaaac aggaactgga aattaaccag cagttaagtc
    11461 agcgtctgat taccgcgact gaaaacggta atcagttgat gcagcaaaac attaaagtca
    11521 aaaactggct ggagcgggcg ctgcaatcgg aacgcaatat taaagagcag attgccgtcc
    11581 tgaagggcag cctgctgttg tctcgtatcc tttaccagca acaacaaacg ctgccctcgg
    11641 cggatgaact ggaaaacatg accaaccgca tcgcggattt gcgtctcgaa cagtttgaag
    11701 ttaaccagca gcgtgatgca ctcttccaga gcgatgcgtt cgtcaacaaa ctggaagaag
    11761 gtcacaccaa cgaagtcaac agcgaagttc acgatgcgtt attgcaagtg gttgatatgc
    11821 gtcgcgaatt gctggatcaa ctcaacaaac agttgggtaa ccagctgatg atggccatta
    11881 acctgcaaat caaccagcag cagttaatga gcgtatcgaa aaacctgaaa tccatcctga
    11941 ctcagcaaat cttttgggtg aacagtaacc gtccaatgga ctgggactgg atcaaagcgt
    12001 tcccgcaaag cctgaaagat gaatttaagt cgatgaaaat cacggtgaac tgggaaaaag
    12061 cctggcctgc cgtttttatc gctttcctcg ccggtttgcc gctgctgttg attgccgggc
    12121 tgatccactg gcgtctgggc tggctgaaag cgtatcaaca gaaactggct tccgctgtgg
    12181 gttccctgcg taacgatagc cagctcaaca caccaaaagc gatccttatc gacctgatcc
    12241 gcgcgctgcc ggtgtgcctg attattctcg cggttggcct gattctgttg accatgcagc
    12301 tcaacatcag cggactgcta tggtcgttca gtaaaaaact ggcgattttc tggctggtgt
    12361 ttggcctgtg ctggaaggta ctggagaaaa atggcgttgc cgtgcgtcac ttcggcatgc
    12421 cggagcagca gaccagccac tggcgtcggc aaattgtccg catcagtctc gcgttgctgc
    12481 ctatccattt ctggtctgtg gtggcagaac tttccccgct gcatctgatg gatgatgtgc
    12541 tggggcaggc gatgattttc ttcaacctgc tgctgattgc cttcctggta tggccaatgt
    12601 gccgcgaaag ctggcgtgat aaagagtcgc acaccatgcg actagtcacc attaccgtgc
    12661 tgtcgattat cccgattgca ttgatggtgc tgactgccac gggttatttc tacaccacgc
    12721 tgcgcctctc cgggcgttgg attgaaaccg tttatctggt gatcatctgg aacttgctct
    12781 accagacggt attgcgcggc ttaagcgtag cggcgcggcg tatcgcctgg cgtcgtgcgc
    12841 tggcgcgtcg gcagaatctg gtgaaagagg gcgcagaagg tgccgaaccg ccggaagaac
    12901 ccaccattgc actggagcaa gttaaccagc agacgctgcg tattaccatg ttgctgatgt
    12961 ttgcgctgtt cggtgtcatg ttctgggcaa tttggtccga tttgatcacc gtgttcagct
    13021 atctcgacag catcacgctc tggcattaca acggcactga agctggcgct gcggtggtga
    13081 aaaacgtcac catgggcagt ctgttgtttg cgattatcgc ctcaatggtg gcctgggcgt
    13141 tgattcgcaa cctgcctggt ttactggaag tgctggtgct ctcgcgactg aatatgcgcc
    13201 agggcgcgtc gtatgccatt actaccatcc ttaactacat cattattgcc gttggtgcga
    13261 tgacggtgtt cggatcgctg ggcgtctctt gggataaact ccagtggctg gccgcagcat
    13321 tatccgtagg tcttggtttt ggtttacaag aaattttcgg taacttcgtc tccggtttga
    13381 tcattctgtt cgaacgtccg gtgcgtattg gcgatacggt aaccattggt agcttctcgg
    13441 ggacggtaag taagatccgt attcgtgcga caacgattac cgatttcgat cgcaaagaag
    13501 tgatcatccc gaacaaagcg tttgttaccg agcgtctgat caactggtcg ttgactgaca
    13561 ctactacgcg tctggtgatc cgtctcggcg tggcctatgg ctccgatctg gaaaaagtgc
    13621 gtaaagtgtt actgaaggcg gcgactgagc acccaagggt gatgcacgaa ccaatgccgg
    13681 aagtcttctt tacggcattt ggtgccagca cgttggatca tgagctgcgt ctgtatgtgc
    13741 gtgaactgcg tgaccgtagc cgtactgtcg atgagctgaa ccgtactatc gatcagctgt
    13801 gccgtgaaaa cgacatcaac attgccttta accagcttga agtgcatctg cacaacgaga
    13861 agggcgatga ggtgacggaa gtaaaacgcg actacaaagg cgacgatccg acaccagcgg
    13921 ttgggtaata accaaagggg gcgacattta gttgccccga gattactgac aaagtgcgcg
    13981 ttgttcatgc cggatgcggc gtgaccgcct tatccggcct acgaaaccgc aagaattcaa
    14041 tatattgcag gagcggtgta ggcctgataa gcgtagcgca tcaggcagtt ttgcgtttgc
    14101 ccgcaatctt aggggacatt tagcggcccc atttatttct cacttttccg cctcatcatc
    14161 gtgcgttaat ttcttttcat aatcacgctt tacaatatcc agcgcgcgca gcacggtact
    14221 ggcagggatc tgattttcct ccagcagcac aatcaaatcg acagccagtt tgacatcgtc
    14281 aggggcattt tcaagtgaca tactctttcc attgctagcg ggttaagcgc gctagcctgt
    14341 tttcgatttt ttccagcgca tggcggcagc gtgccaggcg cgcttcatag gcttccactt
    14401 cacgatgcag cgtttgctgt tccacgagat cggtcacccg cgccaggcgg gctctgcgtt
    14461 cggcaaccat ctcacgtagc cgccgctcaa aatcctgatg ctgaatacgt ttacgctgcc
    14521 agcgggcaat tttcggtggc gcactgtccc actcgcgcaa tgaccaggcg gcggcttcac
    14581 gcgcgatggc ttccagttgt gccgccagat gttccgccag ccaggccact tgcggcagct
    14641 gttgctgctc aactgcatga cgaagcgcag ccagattatc gcccgcctcg tcgagacaag
    14701 cctgtagtgt tgtcgcacga gtctgaaaaa gatgcctgtc gaaacgagca cttagcgtgg
    14761 cgaactgtga caccggggca caacgctgac gcagcgtagc gagctgtcct tccagttttt
    14821 ccagcagcag ggcggttttc acgataagac ctcaatgaaa atgataattg ttatgctaaa
    14881 gtagccactc tgtaagctga caacactcaa tatgcaacga atcattttaa tcatcattgg
    14941 ctggctggcg gtagtgctgg gtacgctggg cgtggtatta ccggtattac cgacgacgcc
    15001 gtttatcctg ctggcggcct ggtgctttgc ccgttcttcc ccgcgctttc acgcctggtt
    15061 gctgtaccgc tcatggtttg gcagctatct acgtttctgg cagaaacatc atgcgatgcc
    15121 gcgcggcgtc aaaccgcggg cgattttgct tattttgctc acgtttgcca tttctctgtg
    15181 gttcgtccag atgccatggg tgcgcatcat gttgctggta attctcgcct gtttgctttt
    15241 ctatatgtgg cgaattccgg tgattgatga aaagcaagaa aagcactgaa gcacaacaat
    15301 cgcagttgca attattgcgt acagccagta tattctggcg ttttcgagca caggcgcagg
    15361 cggtcaaagg ttaaacaaca gttacttttg atacgtttaa aacgcgccgt gagtaccacc
    15421 gtaacaagca ggcatacact tatgaccgcg actgcacagc agcttgagta tctcaaaaat
    15481 agcatcaaaa gcattcagga ctacccaaaa cccggcattc ttttccgcga tgtcaccagc
    15541 ttactggaag acccgaaagc ttacgctctc agcatcgact tgctggttga gcgttacaaa
    15601 aatgcgggca ttaccaaagt tgtcggcacc gaagcgcgtg gcttcttgtt tggcgctccg
    15661 gtagctctgg gtctgggcgt tggctttgta ccggtccgta aaccgggcaa actgccgcgt
    15721 gaaaccatca gtgaaaatta cgacctggaa tacggcaccg atcagctgga aatccacgtt
    15781 gatgccatca aaccgggcga caaagttctg gtggtggacg atctgctggc aaccggcggc
    15841 actatcgaag cgaccgttaa actgatccgt cgtctgggtg gtgaagtggc tgacgctgcg
    15901 ttcattatca acctgttcga tctcggcggc gaacagcgtc tcgaaaaaca gggcattacc
    15961 agctacagcc ttgtcccgtt cccgggccat taattatcgc cagtcttgtg ctgcccacgc
    16021 tacggacagc acaagatgtg cattcagcct cgccgttctg acggggctgt gttagcatta
    16081 ccccttcgtg aatccacctt ccagcgtttc agagcctgcc aatgagttat caggttttag
    16141 cccgaaaatg gcgcccacaa acctttgctg acgtcgtcgg ccaggaacat gtgctgaccg
    16201 cactggcgaa cggcttgtcg ttagggcgta ttcatcatgc ttatcttttt tccggcaccc
    16261 gtggcgtcgg aaaaacctct atcgcccgac tgctggcgaa ggggctaaac tgcgaaaccg
    16321 gcattaccgc gacgccgtgc ggcgtgtgcg ataactgtcg tgaaatcgag caggggcgct
    16381 ttgtcgatct gattgaaatc gacgccgcct cgcgcaccaa agttgaagat acccgcgacc
    16441 tgctggataa cgtccagtac gctccggcgc gtggtcgttt caaagtttat ctgatcgacg
    16501 aagtgcatat gctgtcgcgc cacagcttta acgcactgtt aaaaaccctt gaagagccgc
    16561 cggagcacgt taagtttctg ctggcgacga ccgatccaca gaaattgccg gtgacgattt
    16621 tgtcacgctg tctgcaattt catctcaagg cgctggatgt cgagcaaatt cgccatcagc
    16681 ttgagcacat cctcaatgaa gaacatatcg ctcacgagcc gcgggcgctg caattgttgg
    16741 cgcgcgccgc tgaaggcagc ctgcgagatg ccttaagtct gaccgaccag gcgattgcca
    16801 gcggtgacgg ccaggtttca acccaggcgg tcagtgcgat gctgggtacg cttgacgacg
    16861 atcaggcgct gtcgctggtt gaagcgatgg tcgagggcaa cggcgagcgc gtaatggcac
    16921 tgattaatga agccgctgcc cgtggtatcg agtgggaagc gttgctggtg gaaatgctcg
    16981 gcttgttgca tcgtattgcg atggtacaac tttcgcctgc tgcacttggc aacgacatgg
    17041 ccgccatcga gctgcggatg cgtgaactgg cgcgcaccat accgccgacg gatattcagc
    17101 tttactatca gacgctgttg attggtcgca aagaattacc gtatgcgccg gaccgccgca
    17161 tgggcgttga gatgacgctg ctgcgcgcgc tggcgttcca tccgcgtatg ccgctgcctg
    17221 agccagaagt gccacgccag tcctttgcac ccgtcgcgcc aacggcagta atgacgccaa
    17281 ccctggtgcc gccgcaatca gcgccgcagc aggcaccgac tgtaccgctc ccggaaacca
    17341 ccagccaggt gctggcggcg cgccagcagt tgcagcgcgt gcagggagca accaaagcaa
    17401 aaaagagtga accggcagcc gctacccgcg cgcggccggt gaataacgct gcgctggaaa
    17461 gactggcttc ggtcaccgat cgcgttcagg cgcgtccggt gccatcggcg ctggaaaaag
    17521 cgccagccaa aaaagaagcg tatcgctgga aggcgaccac tccggtgatg cagcaaaaag
    17581 aagtggtcgc cacgccgaag gcgctgaaaa aagcgctgga acatgaaaaa acgccggaac
    17641 tggcggcgaa gctagcggca gaagccattg agcgcgaccc gtggacggca caggtgagcc
    17701 aactttcgct accaaaactg gtcgaacagg tggcgttaaa tgcctggaaa gaggagagcg
    17761 acaacgcagt atgtctgcat ttgcgctcct ctcagcggca tttgaacaac cgcggcgcac
    17821 agcaaaaact ggctgaagcg ttgagcatgt taaaaggttc aacggttgaa ctgactatcg
    17881 ttgaagatga taatcccgcg gtgcgtacgc cgctggagtg gcgtcaggcg atatacgaag
    17941 aaaaacttgc gcaggcgcgc gagtccatta ttgcggataa taatattcag accctgcgtc
    18001 ggtttttcga tgcggagctg gatgaagaaa gtatccgccc catttgatcg taagcacagc
    18061 ttacgttcgt catccttaac gtgattgaga gagaaaccta tgtttggtaa aggcggtctg
    18121 ggtaacctga tgaagcaagc ccagcagatg caagaaaaaa tgcagaaaat gcaggaagag
    18181 atcgcgcagc tggaagttac cggcgaatct ggcgcaggtc tggtaaaagt gaccatcaac
    18241 ggtgcacaca actgccgtcg cgtagagatc gacccgagcc tgctggaaga cgacaaagag
    18301 atgctggaag acctggtggc tgcagcattc aacgacgcag cacgtcgtat tgaagaaacg
    18361 cagaaagaaa aaatggcctc tgtatcctcc ggaatgcagc tgccgcctgg ctttaagatg
    18421 ccgttctgat gcaaaccagc ccgctgttaa cacagcttat ggaagcactg cgctgtctgc
    18481 cgggcgttgg cccgaagtcg gcgcagcgta tggcgttcac gctgcttcag cgcgatcgta
    18541 gcggcgggat gcgtctggcg caggcgctca cccgggcgat gtcggaaatc ggccactgcg
    18601 ccgattgccg cactttcacc gaacaggaag tctgtaacat ctgttcgaat ccgcgtcgtc
    18661 aggaaaacgg tcaaatctgt gtggtggaga gtccggcgga catctacgcc attgagcaga
    18721 cggggcagtt ttctggtcgt tattttgtgt tgatggggca tctgtcaccg ctggacggca
    18781 tcggtccgga tgatatcggg cttgatcgtc tggaacagcg tctggcagag gaaaaaatca
    18841 ctgaagtgat cctcgccacc aatcccacgg ttgaaggtga agctaccgct aactacattg
    18901 ccgagctttg cgcgcaatat gacgtggaag ccagccgaat cgctcatggc gtaccggttg
    18961 gcggcgaact ggaaatggtc gatggcacca cgctgtcaca ctccctcgcc gggcgtcata
    19021 agattcgttt ttaagcaaac gagagcagga gcacctgctc tcgcttgaaa ttattctccc
    19081 ttgtccccat ctctccctca tcctgttttt aaccttaaaa tggcattatt gaggtagacc
    19141 tacatgaaag gacaagaaac tcgtggtttt cagtcagaag tgaaacagct tctgcacctg
    19201 atgatccatt ctctctattc caataaagaa atcttcctgc gtgagcttat ctctaacgcc
    19261 tccgatgcgg cggacaagct gcgtttccgt gcgctatcta acccggacct gtacgaaggt
    19321 gatggcgaac tgcgcgttcg tgtctctttc gataaagaca agcgcacgct gaccatctcc
    19381 gataacggcg tggggatgac ccgcgacgaa gtgattgacc atctggggac tatcgctaaa
    19441 tccggtacca aatcattcct cgaatccctg ggttctgacc aggcgaaaga cagccagctg
    19501 atcggtcagt ttggtgttgg tttctactct gcgtttatcg tggccgacaa agtgaccgtg
    19561 cgtactcgcg cggcaggcga aaaaccagaa aacggcgtct tctgggaatc ggctggcgaa
    19621 ggtgaatata ccgttgccga catcaccaaa gaagatcgtg gtactgaaat caccctgcat
    19681 ctgcgtgaag gcgaagacga gttcctcgat gactggcgcg tgcgttccat catcagcaaa
    19741 tactccgacc atatcgcgct gccggtagag atcgaaaaac gcgaagagaa agatggcgaa
    19801 accgttatct cctgggcaaa aatcaacaaa gcgcaggcgc tgtggactcg taacaagtcg
    19861 gaaatcaccg atgaagagta taaagagttc tacaaacaca tcgcccacga ctttaacgat
    19921 ccgctgacct ggagccacaa ccgcgttgaa ggtaagcagg agtacaccag cctgctgtac
    19981 atcccgtccc aggctccgtg ggatatgtgg aaccgtgatc ataaacacgg cctgaaactg
    20041 tacgttcaac gcgtgtttat catggacgac gctgaacagt tcatgccgaa ctatctgcgc
    20101 ttcgtgcgtg gtctgattga ctccagcgat ctgccgctga acgtttcccg tgaaatcctc
    20161 caggacagca cggtaacgcg taacctgcgc aatgcgctga ccaagcgtgt gctgcaaatg
    20221 ctggaaaaac tggcgaaaga cgacgcggaa aaataccaga ccttctggca acagtttggc
    20281 ctggtactga aagaaggtcc ggcggaagat ttcgctaacc aggaagcgat cgccaaactg
    20341 ctgcgttttg cttctactta taccgattct tctgcgcaga ccgtgtctct ggaagactac
    20401 gtttcccgca tgaaagaagg gcaggagaaa atctactaca tcactgcaga cagctatgcg
    20461 gcagcgaaga gcagcccgca cctggaactg ctgcgtaaga aaggcatcga agttctgctg
    20521 ctttccgacc gcatcgatga gtggatgatg aactatctga ctgagttcga cggtaaaccg
    20581 ttccagtcgg tgtctaaagt tgacgagtcg ctggaaaaac tggctgacga agttgatgag
    20641 agcgcgaaag aagcggagaa agcactgact ccgttcatcg accgcgtgaa agctctgctc
    20701 ggcgagcgcg tgaaagatgt ccgtctgact caccgtctga ccgatacgcc agcgatcgtt
    20761 tcgaccgacg cggacgaaat gagcactcag atggcgaaac tgttcgctgc ggcgggccag
    20821 aaagtgccag aagtgaaata catcttcgaa ctgaacccgg atcacgtact ggtgaaacgt
    20881 gcggcagata ctgaagatga agccaagttc agcgagtggg tagaactgct gctggatcag
    20941 gcgctgctgg cagaacgcgg cacgctggaa gatccgaacc tgtttattcg tcgtatgaac
    21001 cagctgctgg tttcctgatg tcatgccgga tgacctttgt gtcatccggc atttttcttt
    21061 tcatcatctg tactttccgc aaattatctc gccattaacc gtttcagcct caggtgcctt
    21121 tcttgaggca atcgcctgtt ggtggtattg tttatcgctt tttcaaaaaa atcgacacat
    21181 tttaagggga ttttcgcaat gcgtatcatt ctgcttggcg ctccgggcgc ggggaaaggg
    21241 actcaggctc agttcatcat ggagaaatat ggtattccgc aaatctccac tggcgatatg
    21301 ctgcgtgctg cggtcaaatc tggctccgag ctgggtaaac aagcaaaaga cattatggat
    21361 gctggcaaac tggtcaccga cgaactggtg atcgcgctgg ttaaagagcg cattgctcag
    21421 gaagactgcc gtaatggttt cctgttggac ggcttcccgc gtaccattcc gcaggcagac
    21481 gcgatgaaag aagcgggcat caatgttgat tacgttctgg aattcgacgt accggacgaa
    21541 ctgattgttg atcgtatcgt aggccgccgc gttcatgcgc cgtctggtcg tgtttatcac
    21601 gttaaattca atccgccgaa agtagaaggc aaagacgacg ttaccggtga agaactgact
    21661 acccgtaaag acgatcagga agaaaccgtg cgtaaacgtc tggttgaata ccatcagatg
    21721 actgcaccgc tgatcggcta ctactccaaa gaagcggaag cgggtaacac caaatacgcg
    21781 aaagttgacg gtaccaagcc ggttgctgaa gttcgcgctg atctggaaaa aatcctcggc
    21841 taatcaagcc gcagcagatc tgctttcggg caggtctgct catccacctc aggcaatacc
    21901 tctcacagca attagttctt cttcctcact tttccgctac aattatcaac aagttgaatc
    21961 gataagaggc ggtaatgcgt cagactaaaa ccggtatcct gctggcaaac ctgggtacgc
    22021 ccgatgcccc cacacctgaa gcggtaaaac gctatctgaa acaattttta agcgacagac
    22081 gcgtggttga tacctcacgg ttgttatggt ggccgttgct gcgcggcgtg attttgccgc
    22141 tgcgctcgcc gcgtgtggcg aagctgtacg cctctgtctg gatggaagac ggctcgccgc
    22201 tgatggttta cagccgccag caacagcagg cgttggcaca acgtttaccg gatacgccag
    22261 tggcgctggg catgagctac ggctcgccat cactggaaag cgccgtcgat gaactcctgg
    22321 cagagcatgt agatcatatt gtggtgctgc cgctttatcc gcaattctcc tgttctacgg
    22381 tcggtgcggt atgggatgaa ctggcacgca ttctggcgcg caaacgtagc attccgggga
    22441 tatcgtttat tcgtgattac gccgataacc acgattacat taatgcactg gcgaacagcg
    22501 tacgcgcttc ttttgccaaa catggcgaac cggatctgct gctgctctct tatcatggca
    22561 ttccccagcg ttatgcagat gaaggcgatg attacccgca acgttgccgc acaacgactc
    22621 gcgaactggc ttccgcactg gggatggtac cggaaaaagt gatgatgacc tttcagtcgc
    22681 gctttggccg ggaaccctgg ctgatgcctt ataccgacga aacgctgaaa atgctcggag
    22741 aaaaaggcgt aggtcatata caggtgatgt gcccgggctt tgctgcggat tgtctggaga
    22801 cgctggaaga gattgccgag caaaaccgtg aggtcttcct cggtgcgggc gggaaaaaat
    22861 atgaatatat tccagcgctt aatgccacgc cggaacatat cgaaatgatg gctaatcttg
    22921 ttgccgcgta tcgctaaagc tgagcggtaa agaactgagc gccgtcgcga agagcctcgt
    22981 cggcggtttt catcatccgt gaataatgca aaaaggcatg cagcgtacct gggtagagtt
    23041 tgaactcaca gggctgctga tgcgccgcta acgtctggta aagcagacgg ctgtcatcca
    23101 gcagcggatc gaactcctcc ccggcaataa aacagggcgg aacttcgcga gtgagatcat
    23161 tattaaacag acagtaatac ggtgactcgc ggtccgcgtc gttgcttaaa tacgcctctt
    23221 catacatctg caaatcctgt tgcgttaagc catcccagac accgcccaac agacgacgag
    23281 tcacggaatc ccgtaatccg taaagcccat accacagcaa aacacccgca attttaccgc
    23341 aatcgatctg tttatcacgc aaccacaacg cactggcgag cgccagcatg gcacctgcgg
    23401 aatcaccggc aaagccaatg cgggacatat tgatttgata atcctccgcc tgctggtgga
    23461 aataacaaca agcagccaca atttcctcta tcgcttgtgg aaaacgcgct tcaggtgaaa
    23521 gggtgtaatc aataccaatc accgtacatt ggctgtagct tgccagcagg cgcatgatgc
    23581 gatcgtgggt atcgagattg ccgagaataa aaccgccttc catgcaaata aaatagcgtc
    23641 gccgggctat ctggctgcgg acaaaagaga cgtgtttcca cctgcccata ttttgttgga
    23701 accatgtaag ctctggttgc catttctgga gcgcccgcat tccagaatcg gcgctcaagc
    23761 gtgtaatact gtcgttgctc agcaatcgtt cccgttgcgg gccaggacgg taaatccggc
    23821 tgaagagtat tcacaacggt cttcatttca gcagaaataa ggtccagaac aggtagtttg
    23881 ttttccggct tcatacaaaa actcctttca aattacgtca ttgtaaggaa ccactgccat
    23941 gaaaatgcga tcccgcctgc tgatattgaa actggctgcg tctcgcgcgc tcccgtcaga
    24001 ttgtgttaac attcgccgct cagttaacca cccgtaaaaa caaccatgaa atttcccggt
    24061 aaacgtaaat ccaaaaatta cttccctgta aatgcacgcg atccgctgct tcagcaattc
    24121 cagccagaaa acgaaaccag cgccgcctgg gtcgtaggta ttgaccagac gctggtcgat
    24181 attgaagcga aagtggatga tgaattcatt gagcgttatg gattaagcgc cgggcattca
    24241 ctggtgattg aggatgacgt agccgaagtg ctttatcagg agctaaaaca gaaaaacctg
    24301 attacccatc agtttgcggg tggcaccatt ggtaacacca tgcacaacta ctcggtgctc
    24361 gcggacgacc gttcggtgct gctgggcgtc atgtgcagca atattgaaat tggcagttat
    24421 gcctatcgtt acctgtgtaa cacttccagc cgtaccgatc ttaactatct acaaggcgtg
    24481 gatggtccga ttggtcgttg ctttacgctg attggcgagt ccggggaacg tacctttgct
    24541 atcagtccag gccacatgaa ccagctgcgg gctgaaagca ttccggaaga tgtgattgcc
    24601 ggagcctcgg cactggttct cacctcatat ctggtgcgtt gcaagccggg tgaacccatg
    24661 ccggaagcaa ccatgaaagc cattgagtac gcgaagaaat ataacgtacc ggtggtgctg
    24721 acgctgggca ccaagtttgt cattgccgag aatccgcagt ggtggcagca attcctcaaa
    24781 gaccacgttt ctatccttgc gatgaacgaa gatgaagccg aagcgttgac cggagaaagc
    24841 gatccgttgt tggcatctga caaggcgctg gactgggtag atctggtgct gtgcaccgcc
    24901 gggccaatcg gcttgtatat ggcgggcttt accgaagacg aagcgaaacg taaaacccag
    24961 catccgctgc tgccgggcgc tatagcggaa ttcaaccagt atgagtttag ccgcgccatg
    25021 cgccacaagg attgccagaa tccgctgcgt gtctattcgc acattgcgcc gtacatgggc
    25081 gggccggaaa aaatcatgaa cactaatgga gcgggggatg gcgcattggc agcgttgctg
    25141 catgacatta ccgccaacag ctaccatcgt agcaacgtac caaactccag caaacataaa
    25201 ttcacctggt taacttattc atcgttagcg caggtgtgta aatatgctaa ccgtgtgagc
    25261 tatcaggtac tgaaccagca ttcacctcgt ttaacgcgcg gcttgccgga gcgtgaagac
    25321 agcctggaag agtcttactg ggatcgttaa gttatcgtcg gttcgtaggc cagataaggc
    25381 gttcacgccg catctggcat ttggctctcg atgcctgatg cgacgctggc gcgtcttatc
    25441 atgcctacat atttttcata ttttacatcc ggcaaccacc gtttaccccg tcaccacttc
    25501 acccgccggt ggcgtttcca gcagttccag catagtacgg gcaatttcac gttcacccat
    25561 cactacctga ttcgcgccac gttcggtgat atacgtcact tcatcgtcat aatgggcgcg
    25621 ggcgataatc tcaatatccg ggtttttcgc gcgggcagac gccacaatct cacccgcttc
    25681 ataaccgttg gggatcgtca ggatcagcca ttttgcacat tccagatgtg ccagttgcat
    25741 aatttcttcg ttcgctgcat tgcccaatac agcgcggacc ccacgctctc gcagctcatc
    25801 aacacgggtt cgtgacgtct caatcaccac cagcggaata tcagaggcga gcaatttctc
    25861 ccccagcagg ctgcctacac gaccgtaacc caccaatagc gcatggttgc aaatatccac
    25921 tgggatctgt ttctcttctt cgattgcctc ttccagcgtc tgctcttcca gcgtttcggt
    25981 cttcgccaga tatttctcca gtagtgcgaa cagtaccggg ttgagcataa tcgacaggat
    26041 cgcccctgcc agtaccaggt tttgtccggc ctgcggcagt aaattcaatg ccattcccag
    26101 tcccgccagg ataaacgcga actcaccaat ctgcgccagg ctggcggcga tggttaatgc
    26161 cgtacgttgg gagtgaccaa acagacggac aaggaacagg gccgcgaccg atttaccaaa
    26221 cagaataatt gccagcgtcg ccagcactgc cagcggttgc tgaatcagaa ttaacggatc
    26281 aaacaacatc ccgactgaga caaaaaacag caccgcaaac gcgtcgcgca atggcaacgt
    26341 atcgtgggcg gcacggtgac tcaattcaga ctcgttcagt accatcccgg caaagaacgc
    26401 accgagtgca aaggagacat caaacagctc taccgcacca aaggcaatcc ctaacgccag
    26461 cgccagcact gacagggtaa acagctcgcg agaaccggtt gccgcgctgc gtgccataat
    26521 ccacggcacc agacggcgac ctaccagcat cataatggcg ataaatgcga tcaccttgcc
    26581 gatggtgatc cccatatcga ctgcaagagt ggcaaagccc acatcgccct gttccatcat
    26641 tcctgccact gcgggcagca acaccagcgt cagaaccatc accaggtctt ccacaatcaa
    26701 ccaaccgatg gcgatttgcc cacgctgact gtctattaat tgccgttctt caagtgcgcg
    26761 cagtaacacc acggtactgg cggtggaaag acataagccg aacacgatac cggtcattaa
    26821 cgaccagccc agcacggcgg agagcgccat acccagcagc gtcgccacgg ctatctgggc
    26881 gatcgcgccg ggaatggcga tggactttac cgccatcaaa tccttgagcg aaaaatgcaa
    26941 accgacgccg aacatcagca aaataacgcc cagttcagcc agttccggcg caagcttggt
    27001 atcggcaaca aagcccggag tgaatggtcc tgccagcaca cccgctaaca gatatcccac
    27061 cagaggagaa atacgtagtt tattggccag catgccgagg ataaaggcga gcacaaggcc
    27121 gccaacaatg gtggtgataa gcggggtggc gtgatgcatt ccgtctcctt ttcctggtgg
    27181 ttattgtcca tttttggccg ggaaaaccaa aattacaggt aatagtttat gacaatttca
    27241 ttgatgatgt ttatgaataa ttgttgaatt ttgcagaaaa atggaattag ctgcaaaaaa
    27301 agcacggatc ggaaaacaga aggcggttta acagaggaaa ggcttatggc gactgtaaaa
    27361 cgatgcagcc aaagtttgcc tttggctgca atgaaatcag tctttatgcc ggttatcagg
    27421 caggaatatg gtcaacatcc ccaatagtgg caggaaagca cagattttat agactaactc
    27481 gatgctggtg tgatcggcga taagccccag aactgccgct cccagacctc ccatgccgaa
    27541 agcaaaaccg aaaaagagtc cagaaatcat accgatacgt cccggaagta gctcctgagc
    27601 gtagaccaga atggcagaga atgccgaagc gaggataaat ccaataatca ccgttaaaac
    27661 ccccgtccag tgcaggcggg cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat
    27721 agagccccaa atcacatatt tccgtccaat tttatcccct acaggcccgc cgatcaccgt
    27781 acctgccgca acggcaaaca ggaaggcaaa cagatgaagc tgggcattct ggatagataa
    27841 tccgaatttt tgcatcagat aaaaggtgta atagctgctg atgctcgcca tatagaaata
    27901 tttcgagaaa atgaggatta acagaatgct gactgccagt accactttgt tgcgcggcag
    27961 tggattgatt atcgtcgctt tgggttttcc tttattcatt cggtgctgtg ccgagtacca
    28021 acggctaatt tgcgccaaca ccacgatcgc cagcagtgcc gcaagcacaa accaggcaac
    28081 gttgcctttg ccataaggcg cgataatcac cgccgccagc aaggggccca gggaactgcc
    28141 aaagttgccg ccgacctgaa agatagattg cgccaggcca tgccgcccgc cggaagccat
    28201 acgggctacg cgagaagatt ccggatgaaa gaccgatgaa ccggtaccga ccagcgccgc
    28261 cgccagcaga actgcgccaa aactgcccgg cagcgcaagc agcaccagac cacttaaggt
    28321 aaagcacatg ccaattggca acgaccacgg catcggatat ttatcggtcc agtagccgac
    28381 cactggttgc agtagcgaag aggcgagctg gaaggtgagg gttatcatgc caatctgcat
    28441 aaatgtcaga gaaaattctg actgaagcag cggataaatc gccagaatca gcgattggat
    28501 catgtcgttc agcagatgtg agaggctgat agcacctaaa ataccaaacg atgttcgggc
    28561 cttggtcgtt gacgcagccg cgcccgccac aggctgggtt tgttcactca ttgccatagg
    28621 aaagtcactt tttcagggtt gcgatgtaaa gaatgatctt atttgtgatt attacccgac
    28681 taacatacct gtatgcgtcg tctgaaggaa gtctcaacgc cgaatacaga atttctaatc
    28741 tggatgcaga tttatcttca ccggacgcag acttgtctat gatgtcgcgt catactattt
    28801 tttaacacgt tgaaatcagg tcagggagag aagtatgaaa ttattgcagc ggggcgtggc
    28861 gttagcgctg ttaaccacat ttacactggc gagtgaaact gctctggcgt atgagcagga
    28921 taaaacctac aaaattacag ttctgcatac caatgatcat catgggcatt tttggcgcaa
    28981 tgaatatggc gaatatggtc tggcggcgca aaaaacgctg gtggatggta tccgcaaaga
    29041 ggttgcggct gaaggcggta gcgtgctgct actttccggt ggcgacatta acactggcgt
    29101 gcccgagtct gacttacagg atgccgaacc tgattttcgc ggtatgaatc tggtgggcta
    29161 tgacgcgatg gcgatcggta atcatgaatt tgataatccg ctcaccgtat tacgccagca
    29221 ggaaaagtgg gccaagttcc cgttgctttc cgcgaatatc taccagaaaa gtactggcga
    29281 gcgcctgttt aaaccgtggg cgctgtttaa gcgtcaggat ctgaaaattg ccgttattgg
    29341 gctgacaacc gatgacacag caaaaattgg taacccggaa tacttcactg atatcgaatt
    29401 tcgtaagccc gccgatgaag cgaagctggt gattcaggag ctgcaacaga cagaaaagcc
    29461 agacattatt atcgcggcga cccatatggg gcattacgat aatggtgagc acggctctaa
    29521 cgcaccgggc gatgtggaga tggcacgcgc gctgcctgcc ggatcgctgg cgatgatcgt
    29581 cggtggtcac tcgcaagatc cggtctgcat ggcggcagaa aacaaaaaac aggtcgatta
    29641 cgtgccgggt acgccatgca aaccagatca acaaaacggc atctggattg tgcaggcgca
    29701 tgagtggggc aaatacgtgg gacgggctga ttttgagttt cgtaatggcg aaatgaaaat
    29761 ggttaactac cagctgattc cggtgaacct gaagaagaaa gtgacctggg aagacgggaa
    29821 aagcgagcgc gtgctttaca ctcctgaaat cgctgaaaac cagcaaatga tctcgctgtt
    29881 atcaccgttc cagaacaaag gcaaagcgca gctggaagtg aaaataggca aaaccaatgg
    29941 tcgtctggaa ggcgatcgtg acaaagtgcg ttttgtacag accaatatgg ggcggttgat
    30001 tctggcagcc caaatggatc gcactggtgc cgactttgcg gtgatgagcg gaggcggaat
    30061 tcgtgattct atcgaagcag gcgatatcag ctataaaaac gtgctgaaag tgcagccatt
    30121 cggcaatgtg gtggtgtatg ccgacatgac cggtaaagag gtgattgatt acctgaccgc
    30181 cgtcgcgcag atgaagccag attcaggtgc ctacccgcaa tttgccaacg ttagctttgt
    30241 ggcgaaagac ggcaaactga acgaccttaa aatcaaaggc gaaccggtcg atccggcgaa
    30301 aacttaccgt atggcgacat taaacttcaa tgccaccggc ggtgatggat atccgcgcct
    30361 tgataacaaa ccgggctatg tgaataccgg ctttattgat gccgaagtgc tgaaagcgta
    30421 tatccagaaa agctcgccgc tggatgtgag tgtttatgaa ccgaaaggtg aggtgagctg
    30481 gcagtaatcc gaaagtgccg gatgtttgca tccggcacaa tgcttaatcg cggcgggcga
    30541 tatcagcaaa tttggcatcg aggatctttg ccagatcgcc tgccgccagt tcgatatcca
    30601 gtccgcgctt gccgccggaa acataaatag tggcaaattc ttgtgcgggg gcgtcgataa
    30661 tcgttggcag acgttttttc tgccccagtg ggctaatccc cccaaccagg tatcccgtcg
    30721 aacgctgcgc gaccatcgga tcggccatct caactttctt ggcacccagc gcttttgcta
    30781 cttttttaag atccagttga cctgcgaccg gcgtaacggc cacggcaagg tgtttcatat
    30841 caccgttcac tgccaccagc agcgttttgt agacctgatc cggattcaaa cctaattttt
    30901 tgacgacttc atcgccaaaa ttggtttcag ccggatcgtg ctcgtaggta tggatttgaa
    30961 acgaaatctt gtttttttcg agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt
    31021 aaacaaaaaa acgaagcaag catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc
    31081 ctaagagtat tggcaggatg gtgagattga gcgacaatcg agttacaccg tcgcttaaag
    31141 tgacggcata ataataaaaa aatgaaattc ctctttgacg ggccaatagc gatattggcc
    31201 atttttttag cgcaacattt gcggcaaatt cccttctcca tacaggtgta gtgcaccgac
    31261 cgcgaccaca tatcgccccg gcggcatggc gcgtaattta tcccgccagg cgagatttcg
    31321 ctgatgcatc agcacatcgt acagcgactg actgaacgta ttgggcagcg ttatatcatt
    31381 attttgcggc ggtgcattca gccaccagct catcatttgt tgcagcaacc gtgcgttggt
    31441 atgccagtgg gtcagcgtat cgtccagcag cgccagtcct ttgtcaggga gctggagcaa
    31501 catggcaatc tggttttcag ccccttccag ttcaatcacg ggtttatgtt gttgcttcgc
    31561 cgcctgcaat agctggtaat cgataccgta ttctgcccgc agccccagtt tttgtgcctg
    31621 cgtcgcctga agaaccatcg cgatttgcca cagcggttgg gtagaaaaga gtgagggaga
    31681 aatgcccatc tcctggctaa tgtgctgcag gttttggagt tgctcctcgc taatgcgctc
    31741 ttccagcgcc tcgcaggcag gcaaattagc aaaaggcgta tcgctggtgg aaacatccgc
    31801 ctcgacgagc agcgcatcgg cgtttttgag ctttttgagc aaacgggtgg gcaggggagc
    31861 catatcgtgg ctacccatat gaatactgcc aatcagatga aaatggcgat tgccggggag
    31921 ggtgatatcg atggcaggcc aggtgtaatg attaccgcgc agcgcggccc aaagcgtttt
    31981 tacccggtac aacagatcca tacgacctcc ctttgtgaaa tatcatgcta gcgcgcggtg
    32041 agggatggcg caaatggagt tggggacttg tttaatgtgt ttgtatgatt cagtatgttc
    32101 ttgcatcgct attcacaggg aagcaacagt taaaaaccat gaaacaggca acaagaaaac
    32161 cgactacacc cggagatatt cttctctatg aatatctgga accgctcgat ttgaaaatca
    32221 atgagttagc agagttgctg catgttcatc gtaatcgcgt cagtgcactg atcaataaca
    32281 atcgtaaact caccactgag atggcattcc gtctggcgaa agtttttgat accacagtcg
    32341 attttaggct aaacctccag gcggcagttg atctttggga agttgaaaac aacatgcgca
    32401 cccaggaaga attgggacgg attgaaacag tggctgaata tttggcacgc cgtgaagagc
    32461 gtgcaaaaaa ggtcgcgtaa ggccaaaaca tcgcttcatc tcgtaggccg gataaggcgt
    32521 tcacgccgca tccggcatcc gagcaccatt gcctgatgcg acgcttacgc gtcttatcag
    32581 gcctacaaac ctgtgcctga accgtaggcc ggataaggcg tttacgccgc atccggcagt
    32641 catgcgtcga tgccaaatgc gccgccctaa agcagcgcat ccgcaatgac gtacttattc
    32701 cttcggttta aaccgcagca accggttggc gttactcact acggtaatcg acgagagcgc
    32761 cattgccgct ccggcaacta ccgggttaag cagtgttcca gtaaacggcc acaaaatacc
    32821 ggcggcgacc ggaataccga tgctgttgta gataaaggca ccgagcaggt tctgcttcat
    32881 gttgcgcagc gttgcgcggg aaatcgcgag cgcatccgca acgcccatca ggctatggcg
    32941 catcagggta atcgccgcgg tttcaatggc aacatcactg ccgccaccca tcgcaatgcc
    33001 gacatccgcc tgggccagcg ctggcgcgtc gttaatgccg tcgcccacca ttgccacctg
    33061 acgtccttca ctttgcagac gtttgatcgc ttcggcttta ccgtccggca gcactccggc
    33121 aatcacttca tcaattccag cttctttggc gatcgcattg gcggtggttg ggttatcccc
    33181 ggtcaacatc accagacgat atcccgcttt atgcaggcgt tgcagcgccg ccacgctatc
    33241 actacgcaac ggatcgcgta cagccagcag ggctactgct ttcccgtcaa tcgccagcag
    33301 cacaggcgtt gctccttgcg atgcctgagc tgaaatatcg gcttcaatcg ctttagtatc
    33361 gacttgctga tcatttaaca acgcctgatt acccagcaat aacgcatgac cttctgcttc
    33421 accgctcacg cccagcccgc gcaatgtgcg gaaaccgttg acctgcggta gctgcatatc
    33481 gcctgcttta tcaaggatcg ctcgtgccaa tggatggctg gaaccttgct ccagcgccgc
    33541 cgccagacgc agcgcctgcg cttcgtcaac atcagcaaat gttttcactg cgacaacctg
    33601 cggcttccct tcggtcagcg tcccggtttt atcgaacacg acggtatcga gtgtactggc
    33661 gcgttgcagc gcgtcggcgt cccgcaccag cacgccaaac tcagccgccc gcccgacgcc
    33721 ggaaataatc gacatcggcg tcgccagtcc cagcgcacac gggcaggcga taatcagcac
    33781 cgtagtggca atcaccaggg tatagacaat ctgcggtgcc ggaccaaaga aataccagat
    33841 tgccgcactg acaagcgcaa taaccactac taccggcaca aatgcggctg agattttatc
    33901 cgccagctga ccgatttctg gcttgctgct ctgggcctgg cgcaccatgc gaatgatcct
    33961 tgatagcgtg gtatggctgc caaccgcact ggcacgaaac agcacactgc cgtcctgtac
    34021 cactgtcccg gcatggacgc tatcgccttc gcctttttgc tgcgggattg gttcgcccgt
    34081 cagcatcgct tcatccagcc atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg
    34141 atcgccggtc gtcaggcgca gcaacatacc tggctgcact tctgccagag gcacgctttt
    34201 ttcaccttcg tcagtaacca ggcgcgccgt cggtggggtt aaatcgagta atttctccag
    34261 tgccttagaa gaacgctgac gtgcgcgtgc ttccagcata tggccgagat tgatcagacc
    34321 gataatcatc gcactggctt catagtaaag atgtcgcgct tccatcggga accactgcgg
    34381 ccacaggttg acgctcatcg aatagagcca cgccacgccc gttcccagcg ccaccagcgt
    34441 atccatcgtc gctgcaccgt tcagcaggct tttccatgca ctgcggtaaa aatggccgcc
    34501 ggcgaaaacc atcaccgcca gggttatcag gccgataacc aaccacaggc tgcggttgtc
    34561 ggcggtgacc atcatgttat cgccgatcat cccccagacc atcaccggga tacccaccgc
    34621 cagtgcgaca attgcctgcc agcggaagcg cttcatcgta gcgacggcgg tttcttgctg
    34681 gcgttcgcgg cgtttcgcgt catcttcaat cgcttccgcg ccgtagcccg ctttttccac
    34741 cgcctgcact aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag
    34801 gtttacccgt gcctgagtga cgcccggtac gctttgcagc gcattttgta cgcgggtgac
    34861 acagctggcg cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt
    34921 cgctgccgga agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc
    34981 cagcggttta gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc
    35041 gatcagctgt tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc
    35101 ttgttccaca tccgggcgct gttcaagact ttctttcacg cgtttaacgc agtgaccgca
    35161 ggacaggccg tccaggttca ggtcgatagt ttgtgacata aaacactcct ttaagacagt
    35221 tttgactggc tgtgataaag gttaaacctt ccagaaaggg gaaggtcaag aaattaataa
    35281 accaggcggg taaaagtccg taaagattaa aaaatcggct cgatttgcat caggattaga
    35341 catttatttc tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcct
    35401 gaaaatcggc acaggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag
    35461 atgttagatg caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca
    35521 cttaacggcg gacaaaatgc cgattacatt ccctttctgg cgaatgtact aggtcaactg
    35581 gcggcagtgg ctatcgtgac ctgcgatggc aacgtctata gtgcgggtga cagtgattac
    35641 cgctttgcac tggaatccat ctctaaagtc tgtacgttag cccttgcgtt agaagatgtc
    35701 ggcccgcagg cggtacagga caaaattggc gctgacccga ccggattgcc ctttaactca
    35761 gttatcgcct tagagttgca tggcggcaaa ccgctttcgc cactggtaaa tgctggcgct
    35821 attgccacca ccagcctgat taacgctgaa aacgccgaac aacgctggca gcgaatttta
    35881 catatccaac agcaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg
    35941 gaacaaacaa ccaacttcca taaccgggcc attgcctggc tgctgtactc cgccggatat
    36001 ctctattgtg atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc tacgctcatc
    36061 aatactgttg aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgttg
    36121 acgcataaac gcgttcttca ggccgacaac gtgccgtaca ttctggccga aatgatgatg
    36181 gaagggctgt atggtcgctc cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc
    36241 ggtgtaggtg gcggtattct ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca
    36301 ccaccgctgg acgaagaagg caatagtgtt cgcggtcaaa aaatggtggc atcggtcgct
    36361 aagcaactcg gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg
    36421 gtaacaaacc cctcggtcta tggaacgtcg tttccatcgg tattggggca atggtggggg
    36481 cggggatctt cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctggggcg
    36541 cctttgcttt tggcggtatt gtggcgatgt tttccggtta tgcctatgcg cgtctggggg
    36601 cgagctatcc cagcaatggc ggtattatcg acttttttcg tcgcggatta ggcaacggcg
    36661 tcttttcgct agcgctctcg ttgctatacc tgttgacgct ggcggtgagc atcgccatgg
    36721 ttgcccgtgc ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc
    36781 accttatttt gctctacgcg ttggggatca ttgcggtgat gacgcttttc aactccttaa
    36841 gcaaccatgc ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt
    36901 tattgctgat tattgccggt gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc
    36961 cccccagctc cggtgcgttc ttctcctgta ttgggataac cttccttgcc tatgcgggct
    37021 ttggcatgat ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg
    37081 cgtttctggt ggcgattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc
    37141 ttagcgatgt atcggcatta gagttagaaa aatatgctga taccgccgta gcgcaggctg
    37201 cttctccgct gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga
    37261 cggcttcagg cattaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca
    37321 gcgaacgcga actgccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca
    37381 acatcatcgt cgtggtgttg ataatgctga tgacggcggc gctaaattta gactcactcg
    37441 ccagcgttgc cagcgccacc tttttgatct gctatctggc ggtgtttgtg gtggcgatcc
    37501 gcctgcgtca tgatattcac gcctcgttgc cgattcttat cgttggtacg ttggtgatgt
    37561 tgttggtgat cgttggcttt atctacagcc tgtggtccca gggcagccgt gcgttgatat
    37621 ggattattgg tgcaatctta ctcagcctta ttgtggcaat ggtcatgaag cgtaataaaa
    37681 ccgtataaca cctctctgtg cgcagtactt cctgtattat tgtggtggcg gtcgatattc
    37741 gcactggcaa aaaaacgtgc ttgaatatct gttgaaaccc tttaacaaag cacaggaggc
    37801 gttgcgcgaa cgatgaacat cagcgatgta gcaaaaatta ccggcctgac cagcaaagcc
    37861 attcgcttct atgaagagaa ggggctggtg acgccgccga tgcgcagcga aaacggttat
    37921 cgcacctaca cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc acggcaggtg
    37981 ggctttaacc tggaagagag cggcgagctg gtgaatctgt ttaacgaccc gcagcggcac
    38041 agcgccgacg tcaaacggcg cacgctggag aaggtggcgg agatcgaacg acacattgag
    38101 gagctgcaat ccatgcgcga ccagctgctg gcactggcga atgcctgccc tggcgatgac
    38161 agcgccgact gcccgattat cgaaaatctc tccggctgct gtcatcatcg ggcagggtga
    38221 ttaagacgag acggctcgga tatgtagggt tatcccttct atcgcaatga cttcaacata
    38281 cgtacctgca ccgagatcct cgctggcgct gacaggccat gaactgtcac cgacgcgcat
    38341 atgaccgcgc ccgttgacca gcggagattc cagcacaaaa cgtcggccaa tcagctgctg
    38401 cccgcgctgg tttaaatgac tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca
    38461 tttccaccac agccaggcgg cgagcagcgt caggatggca aacattaccc cttgccactc
    38521 ccaacccagc ggcaccagcc agaccaccag gccggtaatc accgctgcca cgccgctcca
    38581 caacaaataa ccatttccgc ccagcatctc ggctgccagc agcaacccgc cgagactgag
    38641 ccagaaaata tgtggatgaa cgaccattaa ctccatcatg gctgagtccg tttgttggcg
    38701 ctgtctttca ccagctcggc aatcccggca atcgacccca tcaggctgct ggcctctaat
    38761 ggcatcatca ctactttgct gttactggag gaaccgatct gctgtaacgc ttcggtgtat
    38821 ttttgcgcta cgaagtagtt caccgcctga atatcaccgg aggcgatagc ttcagacacc
    38881 attttggtgg cgcgggcttc tgcttcggcg gaacgttcac gcgcttcagc ctgtaaaaac
    38941 gccgactgac gttcgccttc cgctttcagg atttgcgact gtttttcacc ttcggctttg
    39001 aggatttccg cctgacggat cccttccgct tcaagaatgt aagcgcgttt ggtacgttcc
    39061 gctttcatct gcgcgttcat tgaagagata agctctgccg gtgggcgcac gtcgcgaatt
    39121 tcaatacggg tgactttaat cccccacggg ttggtggctt catcgacaat acgcagcagg
    39181 cgtgagttga tgctgtcgcg ctgagagagc atttcgtcaa gttccattga acccaacacg
    39241 gtacggatgt tagtcatggt caggttgatg atcgccagct ccagattgct gacttcatag
    39301 gcagcgcgtg gcgcgtcaat cacctgaata aaacagactg cgtcgatggt aacgttggcg
    39361 ttatctttcg agataacttc ctgggaaggg atatcgagca cttgctccat catattgatc
    39421 ttgcgaccaa tgcgatccat aaacggcacc accagactga gccccggctg taacgttttg
    39481 gtatagcgac caaaacgttc taccgtccat tgatagccct gcggcacgat tttgacgccc
    39541 gcgccgacaa tgaccagcgc gacaaaaatg agaatcggga taaagataag catcggaaaa
    39601 acctcctgtt gtaccgtcca taatcagcaa aattgctgct tgattaaaca aattatacct
    39661 gattactgaa agagagttcc cccttattcc tgcgaaggat aaactgtttt tagtaaaaat
    39721 cagaaaaagg gaacagcgat gcaggaaaat agtcctttgc ttcagctaca aaacgtagga
    39781 tatctggcgg gtgatacgaa gattcttaat aacatcaatt tttcgctgcg tgctggcgaa
    39841 tttaagttaa ttaccggtcc ttctggttgt ggcaaaagta cgctgctaaa aatagttgct
    39901 tcattgatca gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt cagcacacta
    39961 aaaccagaaa tctaccgcca gcaagtctct tactgcgccc agacgccgac gctgtttggc
    40021 gatacggtat acgataatct gatctttccc tggcagatcc gtgaccagca gcctgacccg
    40081 gccatttttc tcgattttct cgaacgcttc gctttgccgg acagcatttt gacgaagaat
    40141 atcgccgagc tatctggtgg tgaaaaacaa cgcatctcat tgattcgtaa cctgcaattt
    40201 atgccgaagg ttttattgct ggatgaaata accagtgcgc tggatgaaag taataaacat
    40261 aacgtcaatg agatgatcca tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg
    40321 acacacgata aagacgaaat taatcatgcg gataaagtga ttacactgca accgcatgcc
    40381 ggagaaatgc aggaagtacg ctatgaactc gcataatatt actaacgaat cattagcact
    40441 ggcattaatg ctggtggtgg tcgcaatctt aattagccat aaagaaaaac tggcgctgga
    40501 gaaagatatt ctctggagcg tcgggcgagc gataattcag ctgattattg tcggctatgt
    40561 gctgaagtat attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat
    40621 ctgctttaat gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat
    40681 ctcatcgttt attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc
    40741 agggtcgatt gaatttatcc cgatgcaggt gatccctgtc gccgggatga ttgccggtaa
    40801 cgccatggta gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca
    40861 gcaacagatt caggagaaac tgagtcttgg cgcgacgccg aagcaggctt cagcgatatt
    40921 gattcgcgac agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg
    40981 cttagtgagt ttaccaggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa
    41041 ggcgattaaa tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac
    41101 cattattgcc tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt
    41161 gactcaattg aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg
    41221 gttcatgtgc cgaacaacgg aatatcaata caacaatgca tacagctggc ggcgatactt
    41281 cgacgccagt gcatcacccg tacccagcgc agcgaggatc tcctggaacg ttttacgcgt
    41341 ctgaccgtct gcggcggtga gatctttacg cagatgcccg aacagcaact ccagcgcctc
    41401 ttcattgcgc ccaacctgat gcagttgcag cgccagttgc gtcgccagtg cggcatcttc
    41461 tggattctcc gccacctgct gttgcaactg ttgaatttcc ggcttatcag ccgcctgctt
    41521 cagcagttcg atttgcgcta ccaggccctg gtagcgggtg tcctgatcct gcaacggaat
    41581 ggttttcagc accgcttccg catcttcaga acggttcagc gcaatcagcg tttctgccag
    41641 cagcaggccg atctccccgt tctgattcga caactgccag gcgtctttca gcaatggcag
    41701 ggcatcggtg taattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc
    41761 ttcgcgcggc agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg
    41821 gaagccatct accggttgcc cgttctggaa cagatacacg gtcggaatcg cgcgcagacc
    41881 aaactgggcg gcaatcatct gctccgcgtc gcagtccagc ttcgccagaa taaattgccc
    41941 gttgtactgc gccgcgaggc tttccaggat cggggttaac tgcaaacagt gctggctacg
    42001 ttcagaccaa aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag
    42061 gttagattcg ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga
    42121 tattttcttt gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc
    42181 catcacgcgc ccaggcagca ggcgctcgag caccattact gcccaggtca ccagcgttac
    42241 cggatagcgc atcttcggct tctcgctaat aaaagcatgg cgcactttgt cgaccaccgc
    42301 ttccggtccc aacgtaaagc gggcggcgat gccgggattt tcgactggtt tatcactttg
    42361 cgtctggttg acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac
    42421 tttaattccg ctgtggcgta gctccatgcg cagcgcgtct gaccacgcct ccagcgcata
    42481 tttactggcc gcgtaagcgc cacgacccgg cgtggagatt aatcccatca ccgatgatgt
    42541 catcacaata cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg
    42601 gtgtgcgccg aaaaagttgg cggaaaactg ctgttccatc tgcgcacggc tgatggtgga
    42661 aagggggcca tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt
    42721 cagggcgatc acctcgtctg ctgcgcgatt aacactttct ggtgaatcca gatcgatcaa
    42781 cacgccggta aatcccatgt tgttcatacg ctcaacatca ttcggttttc ggcaacctgc
    42841 cagcacatga aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga
    42901 acatccggta attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt
    42961 atgagtcatg atttactaaa ggctgcaact gcttcgccat ccagtcggca ataaacggct
    43021 gggcgtcgcg gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct
    43081 cttccataaa aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg
    43141 cgctaaaggc ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca
    43201 atggttcagc gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt
    43261 gctgtggctg aaaaccacgc aaaccatcat tgcgcccagt tcaaccagca cccaacgcgg
    43321 ctgatgctgt ttgagcagag ccggaaggcg cgccagccct tgttgcgagg tgtcgccgct
    43381 gatgctggca ttaactaccg acgttttact ctgccactta tcattcaaca aggcaggcca
    43441 ggccgcgctg gcagacattc gatatccggc gctcaggcta tcacccagaa tcaataacgt
    43501 gtccgctgcg gcggcacgga aggttaacag gaccaggaac aggaagggca aatgccagcg
    43561 gaaaacattg ttgaagttca tcatcttaag aagtccgtcg gtcaggggga gcatgaactc
    43621 tccatcctca ccggagttga gctggttgtc aaacgtggcg agaccatcgc actggtgggc
    43681 gagtcgggat cgggtaagtc aaccttgctg gcgatcctcg ccgggcttga tgacggcagc
    43741 agtggcgaag tgagtctggt gggacaaccg ctacataata tggacgaaga agcgcgggca
    43801 aagttgcgcg cgaagcacgt cggctttgtt tttcagtcat ttatgttaat tcctaccctt
    43861 aacgcgctgg aaaacgtcga gcttccggct ctgctgcgcg gtgagagtag cgcggaaagt
    43921 cgtaacgggg cgaaagcgtt gctcgaacag ttagggctgg gtaaacgtct ggatcatctt
    43981 ccggcacagc tttccggcgg tgaacagcaa cgagtggcgc tggcacgagc ctttaatggt
    44041 cgacctgatg tgctgtttgc cgacgaaccc accggcaacc ttgaccgcca gacgggcgat
    44101 aaaattgccg acctgctgtt ttccctcaac cgtgaacatg gcaccacgtt gattatggtg
    44161 acccacgacc tgcaactggc ggcacgctgc gaccgctgct tacggctggt gaacgggcag
    44221 ttgcaggagg aagcatgatt gcacgttggt tctggcgcga atggcgttcg ccgtcgctat
    44281 taattgtctg gctggcgcta agcctggcgg tggcctgcgt gctggcgctg ggcaatatca
    44341 gcgatcgcat ggagaagggc ttaagccagc aaagccgtga gtttatggcg ggcgatcggg
    44401 cgttgcgcag ttcacgcgaa gtgccgcaag cgtggctgga ggaagcgcaa aagcgcggcc
    44461 tgaaagtcgg caagcagctg actttcgcca caatgacctt tgcaggcgac acaccgcagc
    44521 tggcgaacgt caaagcggtg gatgatatct acccgatgta tggcgatctg caaactaatc
    44581 cccctggcct gaaaccgcag gcgggcagcg tattgctggc cccacgcctg atggcactgc
    44641 ttaacctgaa aacggacgac accattgacg tgggcgatgc caccttgcgg attgccggag
    44701 aagtgattca ggaaccggat tccggtttta accccttcca gatggctccg cgtctgatga
    44761 tgaatctggc ggatgtcgat aaaaccggag ccgtgcagcc ggggagtcgg gtcacctggc
    44821 gttataaatt cggcggcaac gagaaccagc tcgacggcta tgagaaatgg ttgttacctc
    44881 agcttaaacc cgaacaacgc tggtacggtc tggaacagga cgaaggcgcg ctggggcgat
    44941 cgatggaacg ctcgcaacag ttcctgctgc tttcggcgct tctgaccttg ctgctggcag
    45001 tggcagcggt ggcggtagcg atgaatcatt actgtcgcag tcgctacgat ctggtggcga
    45061 tcctcaaaac gctgggggca gggcgagcgc aactgcgtaa gctaatcgtc ggtcagtggt
    45121 tgatggtgct gacgctttca gccgttaccg gtggggccat aggcctgttg ttcgaaaacg
    45181 tgttgatggt gctgctcaag cccgttctgc ctgctgcact accgccagcc agcctctggc
    45241 cgtggctgtg ggcgcttggc accatgacgg tcatctcgct gctggtgggg ctacgaccat
    45301 accgcttgtt gctggcaacg cagcctttac gcgtattacg taatgatgtg gtcgcgaacg
    45361 tctggccgct gaagttttat ctgccgattg tcagtgtggt ggttgtgctg ctgctcgccg
    45421 gattaatggg tggcagcatg ctgctttggg cggtgctggc gggcgcggta gtactggctt
    45481 tgctgtgcgg tgtgctgggc tggatgctgc tgaatgtact tcgccgcatg acgctgaaat
    45541 cgctgcctct gcgcctggcg gttagccgcc tgttacgtca gccgtggtca acgttaagcc
    45601 agctttcggc attttcgctc tcctttatgc tgctggcact gctgctggtg ctgcgtggcg
    45661 atctgctcga ccgctggcag cagcagctac cgccagaaag cccgaactac tttttaatta
    45721 acatcgccac agaacaggta gcaccgctaa aagcgttcct cgcggaacat catatagtcc
    45781 cggaatcatt ttatccggta gtgcgggcgc ggctgacggc gattaacgat aagccgacag
    45841 aaggtaatga agatgaggcg cttaatcgcg aactcaatct tacctggcaa aatacgcggc
    45901 ccgatcataa tccgattgtc gccggtaact ggccgccaaa agccgatgaa gtgtcgatgg
    45961 aagaggggct ggcgaaacgc ttaaacgttg ccctcggtga taccgtgact tttatgggcg
    46021 atacccagga gttccgcgct aaagtgacca gcctgcgcaa agtggactgg gaaagtctgc
    46081 ggcctaattt ctattttatt ttccctgaag gggcattaga cgggcaaccg cagagctggc
    46141 ttaccagttt ccgctgggag aatggcaacg gcatgttgac gcaactcaac cgccagttcc
    46201 cgaccattag cctgttagac attggcgcga ttttaaaaca ggtcggtcag gtgctggagc
    46261 aggtaagtcg ggcgctggag gtgatggtgg tgctggtcac cgcctgcggt atgttgctgt
    46321 tgctggcaca ggtgcaggtg ggaatgcgcc agcgtcatca ggagctggtg gtatggcgca
    46381 cactcggtgc ggggaaaaaa ctgctgcgca cgacattgtg gtgtgagttc gccatgcttg
    46441 ggtttgtttc cggcctggtg gccgcaattg gtgcggaaac ggcactggca gtgttgcagg
    46501 cgaaagtgtt tgatttcccg tgggagccag actggcgatt gtggattgtt ctgccgtgca
    46561 gcggtgcgct gctgctgtcg cttttcggcg gctggctggg tgcgcgactg gttaagggta
    46621 aggcgctgtt caggcagttt gtggggtgat gacaggtgtt agattgcctg atgcgctacg
    46681 cttatcaggc ctacgaagag cgtgcaatat attgattttg caagattgtg taggccggat
    46741 aaggcgttta cgccgcatct ggcatgaaca gtgtgcattt tggcaataaa tatgaaacca
    46801 actgtcatgt cggttttttt aaaggccgaa ggaggtaaac cttcggcctt agctacgttt
    46861 cctcagttat tcaataaact gacggatcag ttccagcagc gcgattagcg ccttaagaac
    46921 gatgagaact ttatcgagaa aagttttcat ccgttgctcc cttaagggag aaagacaatc
    46981 acagcttacc cttacgttgc ctgtaactat gattttcttc atgtgctaga tgaggactgc
    47041 ctctctccaa attagagcac cagaatcagc accacctgta tcctgaccag gacttaaaag
    47101 tacttttccg cactgcggta atcccttgag gcagtatcac tactagcgtc gggattgtat
    47161 cgccaacata gctgtttgaa tatgtaggca tcctgactta taattcactt gctagatgag
    47221 gactgtttaa tttctgcccg gaacaaccaa gtcttgggcg gaaaaaataa aaaaaccgac
    47281 tgtcatgtcg gttttttata tctgtattct ggtgcaacca ttttgttctc acgacaatcg
    47341 ccgtttcaga ataccgccca tcactgcgcc ttaacccata ccgccacttc cgcccactca
    47401 ccgcgaaaga caactttttc cgcttttttc tcaagctgat agcgatacat tgggtcgtaa
    47461 tattctttaa gtaacggcac cagccaggcc agatgaccgt cggtgctgcc ggtggtgagt
    47521 tgcgttgtca gcgccgtatc cagctgggca gcgagttcgt tatagcgctg taaccccagc
    47581 cgacgcttaa tcgccgaaag tccgtgatgc aggtattcgc aatattcctg ccagccctgt
    47641 tcgtcgccat aagcgtgggt aaaatcatga tgcatacgca agaaatactc ttcattcagg
    47701 cgttcaagac ggatctcaaa cggatcttct accaccgcaa tcgccgcctg agtcattcgc
    47761 tcgcgcagac attccggcag gtgattcgaa ccgatcatcc ggctttcatc ttccagcacc
    47821 cacaggcgca aatcctgacg ggcgtcggtt tttagcattt cggcagccag caggttttca
    47881 aaactcgcct ggctaagttg tggttgtaac gtgcgaccaa acgccgaacc gcgatgacgc
    47941 gctagccctt ccagatcaac accgtttggc tgttgttgca ctaatagcgt tttaccgctg
    48001 ccggtacaac cgccaatcag cactatcggt ttttgtgcca gttcaatggt cgcctgaatc
    48061 gcggtctggc gcagtgcctt ataaccgcct tccaccagcg gataatcaat ccccgcttca
    48121 tgcagccagc gttgcacaat atgtgaacgt tgaccgccac gggcgcagca gagaatacct
    48181 tgcggatttt gcaggcacgc tgcccgccag gcgtacatac gctgctgacg aatttcaccc
    48241 gccaccagtt tatgccccag cgccagcgct gcgtctgagc cttgctgttt atagcaggtg
    48301 ccaacggcgg cgcgttcatc gttattcatt aacggcagat tgatagcggc gggcattgcg
    48361 ccttgctcaa actcgatagg ggcgcgaaca tcaataatgg gcgtatcagc aatcagcagg
    48421 gcacgatagt cctgttccgt gtgtctctct tgcatagtta aaagtgaacc tcaaatcagc
    48481 ttgcgcgcta ttttacgcgc caacgcgcaa ggaaacttga tttttaactg cgtgggttgc
    48541 cgaaaatttc taaaaatccg ctgatttccg gcctgtgttg ggtaaacaag gtcacaatat
    48601 cttctaccgc tttgccgctg ccaaatttgc gccatgccag actcaatggc gaaggagggc
    48661 gcatcgttgg gattacccgg ctgaccagtt gttgattatc gatcattgac tggcaaagtg
    48721 attttggcaa aaaaccaatg ccaacacccg ccagatgggc ggcgattttc gtttccatat
    48781 cgggaacaat aatctctttt tgccccggca atcgccaggc gacgcgtttg gttaaggtgc
    48841 gggcgctgtc ttcaatattg accgccggaa agcgacgcaa ctgcgcttct gttagcggct
    48901 cttcaacgtt cgccagcgga tgatccgccg ccatgacaaa tcgccattgc accgatccta
    48961 agggatcaag actaaaggta tttgccagcg cctcagttcc cgtgaccccg atagccagcg
    49021 aaaaaccttc gtacaatagc gagtcccaga cgcccatata gatttgtcgg gagatgtgaa
    49081 actgggtaaa ggggtaacgc tcattcagcc acgccagcaa ctgggcgacg gcctgggggt
    49141 tatagagcag gttgttgatg acaatattca cctggcgttc cacgccatca ttcacctgtt
    49201 gcagctcgct gggcatactt tccagccagc tcagccagtc tctggcctgg gaaagtagat
    49261 gctcgccagc cgctgtcaac gttacgctac gagtcgtacg gaaaaacagc gctactccgg
    49321 tattctcttc cagaagttta atgcgataac tgatcgtcgc cgtggtttta cataatcgtt
    49381 ctgccgcttt tgaaaaactt cctgtttcag caaccgcaat gaaagtccgc aaggtttctg
    49441 gatcgaacat cttcaggtat ccccttttaa atccgcaagt tgcgtgattt tcttatcctc
    49501 tgatttatca gtatttttac atgataatcc tgttcaattt gtggactaaa tctagttttg
    49561 gaaaaacatt ccaacttttg tattgatgtt gttctcttaa ggttttagat tgcctgttat
    49621 tgaaaccaag ctgaccggtc ggcggtggtt gaacggaatt atgttacaag gacaaaaaga
    49681 tgaaacttca ggtattaccg ttaagtcagg aagcctttag tgcttatggc gacgtaatcg
    49741 aaacgcagca acgggatttt ttccatatta acaatggcct ggtggagcgt taccacgatt
    49801 tggcgctggt tgagattctt gagcaagacc gtacgcttat cagcattaac cgcgcgcaac
    49861 cggcgaatct gccgctgacc attcacgaac tcgaacgtca tccgctgggc actcaggcct
    49921 ttatcccaat gaaaggtgaa gtgtttgtgg tggtcgtggc gttaggcgac gacaaaccag
    49981 acctgtcaac gctgcgggcg tttatcacca acggtgaaca gggggtgaat taccatcgta
    50041 acgtctggca tcacccactt ttcgcctggc agcgcgtcac cgattttctg accatcgatc
    50101 gcggcggcag tgacaactgt gatgttgaaa gtattcctga acaggaactc tgttttgcgt
    50161 gacgcctgca accgacttgc ataagataaa ctaattgttc attgtttatg ctcacttgta
    50221 ggtcggagtt aacgtaggta tgacggaagt tagacggcgc ggtaggccag gacaggcgga
    50281 gcctgtggca cagaagggcg cacaggcgtt agagcgggga attgcgattc tgcaatatct
    50341 ggaaaaaagt gggggaagtt cgtcggttag cgatatttct ctcaatctgg atttgccgct
    50401 ctccacgacc tttcgcttgc tgaaggtttt acaagcagcg gattttgtct atcaggacag
    50461 tcaattaggc tggtggcata taggattagg tgtctttaac gtcggtgcgg cgtacatcca
    50521 taaccgcgat gtcctctccg tcgccgggcc gtttatgcgc cgcctgatgt tactttccgg
    50581 cgaaacggtc aatgtcgcga tccgtaacgg caatgaagcg gtattaattg gtcagttaga
    50641 gtgtaaatcg atggtcagga tgtgtgcgcc actgggcagt cgtctgccgc tgcatgcttc
    50701 cggtgcgggc aaagcgctgc tttatccgct ggcggaagag gagttgatga gcatcattct
    50761 gcaaaccggt ttgcagcagt ttacgccaac tacgcttgtg gatatgccca ccttgctgaa
    50821 ggacctggaa caagcgcgtg aactgggcta taccgtagat aaagaagagc atgttgtagg
    50881 tctgaattgc atagcttcag caatttacga tgatgtcggt agcgttgttg ccgctatctc
    50941 catctccggg ccttcatcaa gactgacaga agatcgtttt gtcagtcagg gtgagctggt
    51001 cagagacacc gcccgcgata tcagcacggc gttgggactg aaagcacatc catagtgtct
    51061 gtcgcatccc gctctgcgga gcgggttttt ttgacaaaat ttaaaagttg gaaaattttt
    51121 ccaattaata gaggtaggaa taaaatggca aaaatgagag ccgttgacgc ggcaatgtat
    51181 gtgctggaga aagaaggtat caccaccgcc ttcggcgttc cgggagctgc aattaatccg
    51241 ttctactcag cgatgcgtaa gcacggcggt attcgtcaca ttctggcgcg tcatgtggaa
    51301 ggtgtttcgc acatggcgga aggttatacc cgcgcaacgg cagggaatat cggcgtatgt
    51361 ctggggactt ccggtcctgc gggcacggac atgatcaccg cgctctattc cgcttctgct
    51421 gattccattc ctattctgtg cattaccggc caggcaccgc gcgcccgtct gcataaagaa
    51481 gattttcagg ccgtagatat tgaagcaatt gctaaaccgg tcagcaaaat ggcggttaca
    51541 gttcgtgaag cggcgctggt gcctcgcgtg ctgcaacagg catttcacct gatgcgttct
    51601 ggtcgtccgg gtccggtact ggtggattta ccgttcgacg ttcaggttgc ggaaatcgag
    51661 tttgatcctg acatgtacga accgctgccg gtctacaaac ctgctgccag ccgtatgcag
    51721 atcgaaaaag ctgtagaaat gttaatccag gccgaacgtc cggtgattgt tgccgggggc
    51781 ggggtaatca atgctgatgc agctgcactg ttacaacagt ttgctgaact gaccagcgtt
    51841 ccggtgatcc caacgctgat gggctggggc tgtatcccgg acgatcatga actgatggcc
    51901 gggatggtcg gtctgcaaac cgcgcatcgt tacggtaacg caacgttgct ggcgtccgac
    51961 atggtgtttg gtatcggtaa ccgttttgct aaccgtcata ccggttcggt agagaaatac
    52021 accgaagggc gcaaaatcgt tcatattgat attgagccga cgcaaattgg tcgcgtgctg
    52081 tgtccggatc tcggcattgt ctctgatgct aaagcggcgc tgacactgct ggttgaagtg
    52141 gcgcaggaga tgcaaaaagc gggtcgtctg ccgtgtcgta aagaatgggt cgccgactgc
    52201 cagcagcgta aacgcacttt gctgcgcaaa acccacttcg acaacgtgcc ggtgaaaccg
    52261 cagcgcgtgt atgaagagat gaacaaagcc tttggtcgcg atgtttgtta tgtcaccact
    52321 attggtctgt cacaaatcgc tgcggcacaa atgctgcatg tctttaaaga ccgccactgg
    52381 atcaactgtg gtcaggctgg tccgttaggc tggacgattc cggcggcgct gggggtttgt
    52441 gccgctgatc cgaaacgcaa tgtggtggcg atttctggcg actttgactt ccagttcctg
    52501 attgaagagt tagccgttgg cgcgcagttc aacattccgt acatccatgt gttggtgaat
    52561 aacgcctatc tcggcctgat tcgtcagtca caacgcgctt ttgacatgga ctactgcgtg
    52621 caactcgctt tcgagaatat caactccagt gaagtgaatg gctacggtgt tgaccacgta
    52681 aaagtagcgg aaggtttagg ttgtaaagct attcgggtct tcaaaccgga agatattgcg
    52741 ccagcctttg aacaggcgaa agccttaatg gcgcaatatc gggtaccggt agtcgtggaa
    52801 gttattctcg agcgtgtgac caatatttcg atgggcagcg aactggataa cgtcatggaa
    52861 tttgaagata tcgccgataa cgcagcggat gcaccgactg aaacctgctt catgcactat
    52921 gaataaggga gataaatgat gttacgtttc tctgctaatt tatcgatgtt atttggtgaa
    52981 tatgattttc tcgcccgttt tgagaaagct gcgcagtgtg gttttcgcgg cgttgaattt
    53041 atgtttcctt atgactacga cattgaagaa ttaaaacagg tactggcgag taataaactc
    53101 gaacatacgc tgcacaattt accggcaggt gactgggcgg cgggcgagcg cggtattgcc
    53161 tgtattcctg gccgtgaaga agagtttcgg gatggcgtag cagcagcgat tc
//
LOCUS       DAETPZ010000022        49573 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.022, whole genome shotgun sequence.
ACCESSION   DAETPZ010000022 DAETPZ010000000
VERSION     DAETPZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.022"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..1224
                     /locus_tag="K0W59_001429"
     CDS             <1..1224
                     /locus_tag="K0W59_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBI3022529.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGIIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="yggF"
                     /locus_tag="K0W59_001430"
     CDS             1221..2186
                     /gene="yggF"
                     /locus_tag="K0W59_001430"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBI3022530.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVICEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="K0W59_001431"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="K0W59_001431"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HBI3022531.1"
                     /translation="MSTLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="K0W59_001432"
     CDS             2714..3427
                     /locus_tag="K0W59_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HBI3022532.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="K0W59_001433"
     CDS             complement(3399..4076)
                     /locus_tag="K0W59_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBI3022533.1"
                     /translation="MLTLNKISYRWPGAATDCLCGISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="K0W59_001434"
     CDS             complement(4070..4747)
                     /locus_tag="K0W59_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBI3022534.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5118)
                     /locus_tag="K0W59_001435"
     CDS             complement(4735..5118)
                     /locus_tag="K0W59_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBI3022535.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5134..5442)
                     /locus_tag="K0W59_001436"
     CDS             complement(5134..5442)
                     /locus_tag="K0W59_001436"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022536.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5443..6021)
                     /locus_tag="K0W59_001437"
     CDS             complement(5443..6021)
                     /locus_tag="K0W59_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBI3022537.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="K0W59_001438"
     CDS             complement(6044..6475)
                     /locus_tag="K0W59_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBI3022538.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="K0W59_001439"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="K0W59_001439"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HBI3022539.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="K0W59_001440"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="K0W59_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBI3022540.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="K0W59_001441"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="K0W59_001441"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HBI3022541.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /gene="mscS"
                     /locus_tag="K0W59_001442"
     CDS             10564..11424
                     /gene="mscS"
                     /locus_tag="K0W59_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HBI3022542.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="K0W59_001443"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="K0W59_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HBI3022543.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQHIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="K0W59_001444"
     CDS             12291..13031
                     /locus_tag="K0W59_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HBI3022544.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14148
                     /locus_tag="K0W59_001445"
                     /pseudo
     CDS             13198..14148
                     /locus_tag="K0W59_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="K0W59_001446"
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="K0W59_001446"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HBI3022545.1"
                     /translation="METQWTRMTADEAAEIILHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="K0W59_001447"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="K0W59_001447"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HBI3022546.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="K0W59_001448"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="K0W59_001448"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HBI3022547.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="K0W59_001449"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="K0W59_001449"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HBI3022548.1"
                     /translation="MPNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLGKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKMDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMTEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRAARDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVAGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /gene="argP"
                     /locus_tag="K0W59_001450"
     CDS             complement(19724..20617)
                     /gene="argP"
                     /locus_tag="K0W59_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HBI3022549.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="K0W59_001451"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="K0W59_001451"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBI3022550.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /gene="serA"
                     /locus_tag="K0W59_001452"
     CDS             21915..23147
                     /gene="serA"
                     /locus_tag="K0W59_001452"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBI3022551.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="K0W59_001453"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="K0W59_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HBI3022552.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="K0W59_001454"
     CDS             23768..23827
                     /locus_tag="K0W59_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBI3022553.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23924..24526)
                     /locus_tag="K0W59_001455"
     CDS             complement(23924..24526)
                     /locus_tag="K0W59_001455"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBI3022554.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24547..24730)
                     /gene="ssrS"
                     /locus_tag="K0W59_001456"
     ncRNA           complement(24547..24730)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="K0W59_001456"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24772..25101)
                     /gene="zapA"
                     /locus_tag="K0W59_001457"
     CDS             complement(24772..25101)
                     /gene="zapA"
                     /locus_tag="K0W59_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBI3022555.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25269..25847
                     /gene="ygfB"
                     /locus_tag="K0W59_001458"
     CDS             25269..25847
                     /gene="ygfB"
                     /locus_tag="K0W59_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HBI3022556.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25873..27198
                     /gene="pepP"
                     /locus_tag="K0W59_001459"
     CDS             25873..27198
                     /gene="pepP"
                     /locus_tag="K0W59_001459"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HBI3022557.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHARGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27195..28373
                     /gene="ubiH"
                     /locus_tag="K0W59_001460"
                     /gene_synonym="visB"
     CDS             27195..28373
                     /gene="ubiH"
                     /locus_tag="K0W59_001460"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HBI3022558.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28396..29598
                     /gene="ubiI"
                     /locus_tag="K0W59_001461"
                     /gene_synonym="visC"
     CDS             28396..29598
                     /gene="ubiI"
                     /locus_tag="K0W59_001461"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HBI3022559.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLSEWLR"
     gene            complement(29672..29755)
                     /locus_tag="K0W59_001462"
     CDS             complement(29672..29755)
                     /locus_tag="K0W59_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022560.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30046..31140
                     /gene="gcvT"
                     /locus_tag="K0W59_001463"
     CDS             30046..31140
                     /gene="gcvT"
                     /locus_tag="K0W59_001463"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HBI3022561.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31164..31553
                     /gene="gcvH"
                     /locus_tag="K0W59_001464"
     CDS             31164..31553
                     /gene="gcvH"
                     /locus_tag="K0W59_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBI3022562.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31672..34545
                     /gene="gcvP"
                     /locus_tag="K0W59_001465"
     CDS             31672..34545
                     /gene="gcvP"
                     /locus_tag="K0W59_001465"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HBI3022563.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEEIGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34812..35555
                     /locus_tag="K0W59_001466"
     CDS             34812..35555
                     /locus_tag="K0W59_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI3022564.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGRSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVVQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35612..37045)
                     /gene="bglA"
                     /locus_tag="K0W59_001467"
     CDS             complement(35612..37045)
                     /gene="bglA"
                     /locus_tag="K0W59_001467"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HBI3022565.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37090..37401
                     /locus_tag="K0W59_001468"
     CDS             37090..37401
                     /locus_tag="K0W59_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBI3022566.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLPELKKVIADIYPGQTQFYMIEFKC
                     L"
     gene            37565..38224
                     /locus_tag="K0W59_001469"
     CDS             37565..38224
                     /locus_tag="K0W59_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBI3022567.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38420..39400)
                     /gene="ygfZ"
                     /locus_tag="K0W59_001470"
     CDS             complement(38420..39400)
                     /gene="ygfZ"
                     /locus_tag="K0W59_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HBI3022568.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39643..39909
                     /gene="sdhE"
                     /locus_tag="K0W59_001471"
     CDS             39643..39909
                     /gene="sdhE"
                     /locus_tag="K0W59_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HBI3022569.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39890..40297
                     /locus_tag="K0W59_001472"
     CDS             39890..40297
                     /locus_tag="K0W59_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HBI3022570.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40337..40858)
                     /gene="fldB"
                     /locus_tag="K0W59_001473"
     CDS             complement(40337..40858)
                     /gene="fldB"
                     /locus_tag="K0W59_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HBI3022571.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40970..41866
                     /gene="xerD"
                     /locus_tag="K0W59_001474"
     CDS             40970..41866
                     /gene="xerD"
                     /locus_tag="K0W59_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBI3022572.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41891..42601
                     /gene="dsbC"
                     /locus_tag="K0W59_001475"
     CDS             41891..42601
                     /gene="dsbC"
                     /locus_tag="K0W59_001475"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HBI3022573.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42607..44340
                     /gene="recJ"
                     /locus_tag="K0W59_001476"
     CDS             42607..44340
                     /gene="recJ"
                     /locus_tag="K0W59_001476"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBI3022574.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44431..45529
                     /gene="prfB"
                     /locus_tag="K0W59_001477"
     CDS             join(44431..44505,44507..45529)
                     /gene="prfB"
                     /locus_tag="K0W59_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBI3022575.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45539..47056
                     /gene="lysS"
                     /locus_tag="K0W59_001478"
     CDS             45539..47056
                     /gene="lysS"
                     /locus_tag="K0W59_001478"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBI3022576.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47099..47647)
                     /gene="idi"
                     /locus_tag="K0W59_001479"
     CDS             complement(47099..47647)
                     /gene="idi"
                     /locus_tag="K0W59_001479"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBI3022577.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARILLSAFTQLK"
     gene            47702..47773
                     /locus_tag="K0W59_001480"
     CDS             47702..47773
                     /locus_tag="K0W59_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022578.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47770..47895)
                     /locus_tag="K0W59_001481"
     CDS             complement(47770..47895)
                     /locus_tag="K0W59_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022579.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47897..49345)
                     /gene="uacT"
                     /locus_tag="K0W59_001482"
     CDS             complement(47897..49345)
                     /gene="uacT"
                     /locus_tag="K0W59_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HBI3022580.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        11967 a        12404 c        13348 g        11854 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg ggattattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggccgcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tctgcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg tcgactctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa accgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcagacgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggtgctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agtcgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ctatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgccgcaa
     4021 agacaatccg ttgccgcgcc tggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggcgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgaccacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatttctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc caggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgggta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagcca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctagat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 tgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcccttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcatt gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ttggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcacattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgtgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgtgattg ggctttctcg tttctcgccg ttataaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggtgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgcg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagcc
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgtaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgcaggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ctaggttata
    15961 cgggacaccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg accgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttattgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgacgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccgctacg tgaacgtcgt tttctacggc caggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggc ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcggtca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tccattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttaccc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttaggcat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caggcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gttaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcacagggga ggagcaatcc tcccttaccc ttactcacta aattaggtta
    23881 aagaatcaac gatgtcaatc agggcgatgc aggtgtatcg cccttaccac tcccagactt
    23941 tcgacggtgt aaccaccgca ggaagaggga tatcccactc ttcaacgggg agtttttcca
    24001 ccaactgaca atcatgcgca taacccaccg gttgcgtttt atagtgctgc cagttttgta
    24061 aggtccgatc ataaaaacca ccgcccattc ccaggcgctg accgtactca tcaaaggcga
    24121 ccagcggtgt gatcagcacg tctaatcggg aaaggggtag cacgtcacgc acatccagtt
    24181 ttggctcatg gatcttcaac ctgttcatca ccagttcgct ttgcggatgg taattcagga
    24241 acagcaaatt accggcacta aagggatgca aaactggaag atatacgcgc ttaccggcgc
    24301 gccagagttg ttctattagc ggctgggtgt cgagttcacc atcaaaagag aggaatacag
    24361 cgaccgtatg cgccatcacc accgggggat aagtcatcat ccgggtagcg gcttgttgac
    24421 ccatttccag ctgttgttcc ggcgttaacg cacgacgacg ttgccgaatc attttgcgga
    24481 tttcttgtcg ggataatgtc agtgggagct ctggtagttg cgtcatggct ggtgccagat
    24541 aagaagggaa tctccgagat gccgccgcag gctgtaaccc ttgaaccctt ggttcaaggt
    24601 gaatgtgtcg tcgcagtttt aaggcttctc ggacgaaccg agcatgctca ccaaccgcgg
    24661 agcgccacat tcttgtggta tgaaatatcg gctcagggga ctggcccgct tgcgaacatc
    24721 tcagagaaat tttgtcttca cggttactct accacagtaa accgaaaagt gtcattcaaa
    24781 gttttggtta gttttttcgg tgatgcgacc ttgttcaagt aacgcttgtt ctatggtctg
    24841 ctgcagcatc cgaatacgct gttccatact tgccgcgtag tcacgagtct ttgctttttc
    24901 ttgcgctaac tcatagctga tattcaatgc ggcaatgaag accaactgtt cagtatttgt
    24961 gactctagtg cgttctttca gatcttgcaa ccgttggttc agatcgtccg ctgcctgatt
    25021 caacgcatcc ctttggtcag gcgggcagtt cacacgcagt gaacggccaa aaatttggat
    25081 atcgacgggt tgtgcagaca tgccaccttc ctgctgattg actgcgctgc cttcgtcttt
    25141 agacccatgg tctgcgaagg ggcgacgcta tagctaccct gatgagaaga gacaagccct
    25201 tttctggtcc accagggccc aaagtggtag catatcatga atattcctcc ctttgacgac
    25261 gaatgcttat gtctatacag aacgaaatgc ctggttacaa cgaaatgaac cagtatctga
    25321 accaacaagg gacgggtctg acccctgccg agatgcatgg tttaatcagt gggatgatat
    25381 gtggcggtaa cgatgacagc tcatggctgc cgctacttca cgacctgacg aacgaaggca
    25441 tggctttcgg tcatgagctg gcacaggcac tgcgtaaaat gcactctgcc accagcgatg
    25501 ccctgcagga tgacggcttc ctttttcagc tttatctgcc tgatggcgat gatgtcagcg
    25561 ttttcgatcg ggctgatgca ctggcaggtt gggtcaatca cttcctgctt ggtcttggcg
    25621 ttacgcaacc gaagctggac aaagtgaccg gcgaaaccgg tgaagccatc gacgatctgc
    25681 gtaacatcgc gcagctgggc tacgacgaag acgaagatca ggaagagctt gaaatgtcgc
    25741 ttgaagagat catcgaatac gttcgtgttg ccgcgctgtt atgccacgac acctttactc
    25801 atccgcaacc gaccgcgcca gaagtacaaa aaccgactct acactaaaaa caaaaaacgt
    25861 aaggagagtg ttatgagtga gatatcccgg caagagtttc agcgtcgccg tcaggccctg
    25921 gtggagcaaa tgcaacccgg cagcgccgcg ctgatttttg ctgcaccaga agtaacacgt
    25981 agcgccgaca gcgaataccc ctatcgtcag aacagtgact tctggtactt caccggcttt
    26041 aacgaaccgg aagcggtgct ggtgctgatt aaaagcgatg acactcataa ccacagcgtt
    26101 ctgtttaacc gcgttcgcga cctgacggcg gagatctggt ttggccgtcg cttaggccag
    26161 gatgccgcgc cagagaaact gggcgttgac cgcgcactgg cattcagcga aatcaatcag
    26221 caactttatc aactacttaa cggcctggat gtggtttacc atgcccgggg cgaatatgca
    26281 tatgctgatg aaatcgtgaa cagtgcgctg gaaaaactgc gtaaaggttc gcggcaaaat
    26341 ctcaccgcac cggcaacgat gatcgactgg cgtcctgttg ttcatgaaat gcgcctgttc
    26401 aaatcgccag aagagattgc cgtactccgc cgcgcgggag aaatcaccgc catggcacat
    26461 acacgggcga tggaaaaatg ccgtccggga atgttcgagt accatctgga aggcgaaatt
    26521 caccacgaat ttaaccgcca cggtgcgcgc tatccttcct ataacaccat tgtaggcagc
    26581 ggtgaaaacg gctgcattct gcactacacc gaaaacgaga gtgaactgcg cgacggtgac
    26641 ctggtgttga ttgacgcggg ttgtgaatac aaaggttacg cgggcgatat tacccgcacc
    26701 ttcccggtca acggcaaatt cacccaggcc cagcgtgaaa tctacgacat tgtgctggag
    26761 tctctcgaaa ccagcctgcg cctgtatcgt ccgggaactt ccattctgga agtcactggt
    26821 gaagtggtgc gcatcatggt tagcggcctg gtaaaactcg gcatcctgaa aggtaatgtt
    26881 gatgaactga tcgctcagaa cgcccatcgt cctttcttta tgcatggcct tagccactgg
    26941 ttaggactgg atgtccatga cgttggcgtt tatggtcagg atcgctcgcg cattctggaa
    27001 ccggggatgg tactgaccgt agagcctggt ctgtatatcg cgccggatgc ggatgtgcca
    27061 gaacaatatc gcggtatcgg cattcgtatt gaagacgaca ttgtgattac cgaaaccggc
    27121 aacgaaaacc tcaccgccag cgtggtgaaa aagccggaag aaatcgaagc gttgatggct
    27181 gctgcgagaa agcaatgagc gtaatcatcg tcggtggcgg catggcgggc gcgacgctgg
    27241 cgctggctat ttcccggtta agtcacgggg cgctgccggt acatttgatt gaagcgactg
    27301 cgccagagtc acatgctcat ccgggctttg atggacgagc gatagcgctg gcggcgggta
    27361 cctgtcagca actggcgcgc atcggcgtct ggcaatctct ggctgattgc gcaaccgcca
    27421 tcactaccgt gcatgtcagc gatcgtggtc atgctggatt cgtcaccctc gccgcagaag
    27481 attaccaact ggcggcgctg gggcaggttg tcgaattgca taatgttggg caacggctgt
    27541 ttgcattgct gcgaaaagca cctggcgtaa tgctgcattg ccccgatcgc gtagctaacg
    27601 ttgcccgtac tcagagtcac gttgaagtga cgctggagag cggcgagatg ctgacgggcc
    27661 gcgtgctgat agccgctgat ggcacccatt cagcgttagc caccgcgtgc ggcgttgatt
    27721 ggcagcagga gccttacgaa caactggcag tgattgccaa cgttgctact tctgttgcgc
    27781 acgaaggccg cgcttttgaa cgcttcacgc aacatggccc gctggcgatg ttgccgatgt
    27841 ctgacggacg ctgttcgctg gtctggtgtc atccactgga acggcgcgaa gaggtgctgt
    27901 cgtggagtga cgagaagttt tgccgtgaac tccagtctgc ctttggctgg cgactggggc
    27961 aaattaccca cgccggtaaa cgcagtgctt atccgctggc attaacccgc gccgccagac
    28021 cgattaccca tcgcactgtg ctggtgggca atgcggcgca aactctgcac ccgattgccg
    28081 ggcaagggtt taacctcggt atgcgagatg tcatgagcct tgcggaaacc ctgactcagg
    28141 cgcatgagcg cggagaagac atcggtgatt acggcatatt gtgccgctat cagcagcgtc
    28201 gacagagcga tcgcgaagca accatcggcg tcacggacag cctggtacat ctttttgcca
    28261 accgttgggc accgctggtt gtcgggcgca acatcggact gatgacgatg gaattattca
    28321 ccccggcacg cgatgtgctg gcgcagcgca ccctcggttg ggtggcgcgt tgaaggcggt
    28381 tttaaggaat aaacaatgca aagtgttgat gtagccattg ttggcggcgg catggtgggg
    28441 ctggcggttg cctgcggctt acaggggagc ggcttacgcg ttgccgtact ggagcagcgc
    28501 gtaccggaac ctctggcggt agatgcacta ccacaactgc gcgtttcggc tatcaatgcc
    28561 gccagcgaaa aattactcac ccgtcttggc gtctggcagg acattctcgc tcgtagggcc
    28621 agttgctatc acggcatgga agtgtgggac aaagacagtt ttggtcacat ctcgtttgac
    28681 gatcaaagca tgggctatag ccatcttggg cacatcgttg aaaactcagt gattcactac
    28741 gcgctgtgga acaaagcgca gcagtcgtca gatattactc tgttggcccc cacagaatta
    28801 cagcaggtcg cctggggaga aaatgaaacc ttcctgacgc tgaaagatgg cagcatgtta
    28861 acggcgcgtc tggtgattgg cgcggacggt gctaattcct ggttgcgcaa caaagcagat
    28921 attccgctga ctttctggga ttatcagcat cacgcgctgg tagcgaccat tcgcacggaa
    28981 gaaccgcatg atgcggtggc gcggcaggtt ttccatggcg aaggcattct ggccttttta
    29041 ccgcttagcg atccgcatct ttgctcgatt gtctggtcac tgtcgccaga ggaagcgcag
    29101 cggatgcagc aggcaagtga agacgaattt aatcgcgcgt taaatatcgc ttttgataat
    29161 cgccttggct tatgcaaggt tgagagcgcg cgtcaggtgt tcccgctgac ggggcgttat
    29221 gcgcgccagt ttgccgcaca ccgtctggcg ctggtgggcg acgccgcgca taccattcac
    29281 ccactggcgg ggcagggcgt taatctcgga tttatggacg ccgcagagct gattgctgaa
    29341 ctgaaacggt tgcatcgtca gggtaaagac atcgggcagt acatttacct gcgccgctat
    29401 gaacgtagcc gcaagcacag cgcggcgctg atgctggctg gtatgcaggg ttttcgcgat
    29461 ctcttttccg gcgctaatcc ggcgaaaaaa ctgctgcgtg atattggttt gaaactggcc
    29521 gacacgcttc ctggcgttaa gccgcaactt atccgccagg caatgggatt aaacgatttg
    29581 tctgaatggc tgcgttaaaa atttctcctc tgttgtttat ttgataccca tcacactttc
    29641 atctcccggt tttttcgccg ggagattttc ctcatttgaa ataaactaat ttcacctccg
    29701 ttttcgcatt atattttcta atgccatgat tttttgattt agtgtttttt gacatttttt
    29761 tagctcttaa tattgtctta ttcaaattga ctttctcatc acatcatctt tgtatagaaa
    29821 ttggtgtatt ttttggtttt ttattctgtc gcgatttttg cattttttaa ccataagcta
    29881 atgtgatgat caattttacc ttatggttaa cagtctgttt cggtggtaag ttcaggcaaa
    29941 agagaacgat tgcgttgggg accgggagtg gctccgatgc tgggttttgt ggtgataatt
    30001 tcaccatgaa aaagttgtca gccccgctta ttcaatgagg acaagatggc acaacagact
    30061 cctttgtacg aacaacacac gctttgcggc gctcgcatgg tggatttcca cggctggatg
    30121 atgccgctgc attacggttc gcaaatcgac gaacatcatg cggtacgtac cgatgccgga
    30181 atgtttgatg tgtcgcatat gaccatcgtc gatctccgcg gcagccgcac ccgggagttt
    30241 ctgcgttatc tgctggcgaa cgatgtggcg aagctcacca aaagcggcaa agccctttac
    30301 tcggggatgt tgaatgcctc tggcggtgtg atagatgatc tcatcgtcta ctactttact
    30361 gaagatttct tccgcctcgt tgttaactcc gccacccgcg aaaaagacct ctcctggatt
    30421 acccaacacg ctgaaccttt cggcatcgaa attaccgttc gtgatgacct ttccatgatt
    30481 gccgtgcaag ggccgaatgc tcaggcaaaa gctgccacac tgtttaatga tgctcagcgt
    30541 caggcggtgg aagggatgaa accgttcttt ggcgtgcagg tgggcgatct gtttattgcc
    30601 accactggtt ataccggtga agcgggctat gaaattgcgc tgcccaatga aaaagcggcc
    30661 gatttctggc gtgcgctggt ggaggcgggc gttaagccct gcggtctggg ggcgcgtgac
    30721 acgctgcgtc tggaagcggg catgaatctt tatggtcagg agatggacga aactatttct
    30781 cctttagccg ccaacatggg ctggactatc gcctgggaac cgacagatcg tgactttatc
    30841 ggtcgtgaag ccctggaagt gcagcgtgag catggtacag aaaaactggt tggtctggtg
    30901 atgaccgaaa aaggcgtgct gcgtaatgaa ctgccggtac gctttaccga tgcgcagggc
    30961 aaccagcatg aaggcattat caccagcggt actttctccc cgacgctggg ttacagcatt
    31021 gcgctggcgc gcgtgccgga aggtattggc gaaacggcga ttgtgcaaat tcgcaaccgt
    31081 gaaatgccgg ttaaagtgac aaaacctgtt tttgtgcgta acggcaaagc cgtcgcgtga
    31141 tttacttttt tggagattga ttgatgagca acgtaccagc agaactgaaa tacagcaaag
    31201 aacacgaatg gctgcgtaaa gaagccgacg gcacttacac cgttggtatt accgaacatg
    31261 ctcaggagct gttaggcgat atggtgtttg ttgacctgcc ggaagtgggc gcaacggtta
    31321 gcgcgggcga tgactgcgcg gttgccgaat cggtaaaagc ggcgtcagac atttatgcgc
    31381 cagtaagcgg tgaaatcgtg gcggtaaacg acgcactgag cgattccccg gaactggtga
    31441 acagcgaacc gtatgcaggc ggctggatct ttaaaatcaa agctagcgat gaaagcgaac
    31501 tggaatcact gctggatgcg accgcatacg aagcattgtt agaagatgag taacggcttt
    31561 attcctcttc tgcgggagag gatcagggtg aggaaaattt atgcctcacc ctcactctct
    31621 tcgtaaggag agaggttcac aattcactgc acgtttcagg aaccatcgct catgacacag
    31681 acgttaagcc agcttgaaaa cagcggcgct tttattgaac gccatatcgg accggacgcc
    31741 gcgcaacagc aagaaatgct gaatgccgtt ggtgcacaat cgttaaacgc gctgaccggc
    31801 cagattgtgc cgaaagatat tcagcttgcg acaccaccgc aggttggcgc accggcgacc
    31861 gaatacgccg cactggcaga actcaaggct attgccagtc gcaataaacg cttcacgtct
    31921 tacatcggca tgggttacac cgccgtgcag ctaccgccgg ttatcctgcg taacatgctg
    31981 gaaaatccgg gctggtatac cgcgtacact ccgtatcaac ctgaagtctc ccagggccgc
    32041 cttgaagcac tgctcaactt ccagcaggta acgctggatt tgactggact ggatatggcc
    32101 tctgcttctc ttctggacga ggccaccgct gccgccgaag caatggcgat ggcgaaacgc
    32161 gtcagcaaac tgaaaaatgc caaccgcttc ttcgtggctt ccaatgtgca tccgcaaacg
    32221 ctggatgtgg tccgtactcg tgccgaaacc tttggttttg aagtgattgt cgatgacgcg
    32281 caaaaagtgc tcgaccatca ggacgtcttc ggcgtgctgt tacagcaggt aggcactacc
    32341 ggtgaaattc acgactacac tgcgcttatt agcgaactga aatcacgcaa aattgtggtc
    32401 agcgttgccg ccgatattat ggcgctggtg ctgttaactg cgccgggtaa acagggcgcg
    32461 gatattgttt ttggttcggc gcaacgcttc ggcgtgccga tgggctacgg tggcccacac
    32521 gcggcattct ttgcggcgaa agatgaatac aaacgctcaa tgccgggccg tattatcggt
    32581 gtatcgaaag atgcagctgg caataccgcg ctgcgcatgg cgatgcagac tcgcgagcaa
    32641 catattcgtc gtgagaaagc gaactccaac atttgtactt cccaggtact gctggcaaac
    32701 atcgccagcc tgtatgccgt ttatcacggc ccggttggcc tgaaacgtat cgctaaccgc
    32761 attcaccgtc tgaccgatat tctggcggcg ggcctgcaac aaaaaggtct gaaactgcgc
    32821 catgcgcact atttcgacac cttgtgtgtg gaagtggccg acaaagcggg cgtactgacg
    32881 cgtgccgaag cggctgaaat caacctgcgt agcgatatcc tgaacgccgt ggggatcacc
    32941 cttgatgaaa ccaccacgcg tgaaaacgta atgcagcttt tcagtgtgct gctgggcgat
    33001 aaccacgggc tggagatcga cacgctggac aaagacgtgg ctcacgacag ccgctctatc
    33061 cagcctgcga tgctgcgcga cgacgaaatc ctcacccatc cggtgtttaa tcgctatcac
    33121 agcgaaaccg aaatgatgcg ctatatgcac tcgctggagc gtaaagatct ggcgctgaat
    33181 caggcgatga tcccgctggg ttcctgcacc atgaaactga acgccgccgc cgagatgatc
    33241 ccaatcactt ggacggaatt tgccgaactg cacccgtttt gcccgccgga acaggctgaa
    33301 ggttatcagc agatgattgc gcagctggct gactggctgg tgaaactgac cggttacgac
    33361 gccgtttgta tgcagccgaa ctccggcgca cagggcgaat acgcgggcct gctggcgatt
    33421 cgtcattatc atgaaagccg caacgaaggg catcgcgata tctgcctgat cccggcttct
    33481 gcgcacggaa ctaaccccgc ttctgcacat atggcaggaa tgcaggtggt ggttgtggcg
    33541 tgtgataaaa acggcaacat cgatctgact gatctgcgcg cgaaagcgga acaggcgggc
    33601 gataacctct cctgtatcat ggtgacttat ccttctaccc acggcgtgta tgaagaaacg
    33661 atccgtgaag tgtgtgaagt cgtgcatcag ttcggcggtc aggtttacct tgatggcgcg
    33721 aacatgaacg cccaggttgg catcacctcg ccgggcttta ttggcgcgga cgtttcgcac
    33781 ctcaacctgc ataaaacttt ctgcattccg cacggcggtg gtggtccggg tatgggaccg
    33841 atcggtgtga aagcgcatct ggcaccgttt gtaccgggtc atagcgtggt gcaaatcgaa
    33901 ggcatgttaa cccgtcaggg cgcagtttct gcggcaccgt tcggtagcgc ctctatcctg
    33961 ccaatcagct ggatgtacat ccgcatgatg ggcgcagaag ggctgaaaaa agcaagccag
    34021 gtggcaatcc tcaacgccaa ctatattgcc agccgcctgc aggatgcctt cccggtgctg
    34081 tataccggtc gcgacggtcg cgtggcgcac gaatgtattc tcgatattcg cccgctgaaa
    34141 gaagaaatcg gcatcagcga gctggatatt gccaagcgcc tgatcgacta cggtttccac
    34201 gcgccgacga tgtcgttccc ggtggcgggt acgctgatgg ttgaaccgac tgaatctgaa
    34261 agcaaagtgg aactggatcg ctttatcgac gcgatgctgg ctatccgcgc agaaattgac
    34321 caggtgaaag ccggtgtctg gccgctggaa gataacccgc tggtgaacgc gccgcacatt
    34381 cagagcgaac tggtcgccga gtgggcgcat ccgtacagcc gtgaagttgc ggtattcccg
    34441 gcaggcgtgg cagacaaata ctggccgacg gtgaaacgtc tggatgatgt ttacggcgac
    34501 cgtaacctgt tctgctcctg cgtaccgatt agcgaatacc agtaattcac tgattcgagt
    34561 attttctaaa ggcgcttcgg cgccttttta gtcagatgac aaagtacaaa agtgctcaga
    34621 cagtcccctc gcccctttgg ggagagggtt agggtgaggg gaacaggccg gcactggcgc
    34681 gaatatttac cctcaccccg accatctccc tgaaagggcg agggggaaaa gcgtgccaac
    34741 attgaagatt gagccagttt gttagcaatc tcaaagatac gtcaacgaat taatttttct
    34801 cggaaaaaca aatggctata gcacttgtga ccggtggcag tcgcggtatc gggcgggcaa
    34861 ctgcattact gttggcgcaa gaagggtata cggtggcggt taattatcag caaaacctcc
    34921 acgcggcgca ggaagtgatg aacttaataa tgcaagccgg tggcaaagca ttcgtgctcc
    34981 aggcggatat cagcgacgaa aaccaggtcg ttgcgatgtt tacagcaatc gatcagcacg
    35041 atgaaccgct agcagcgctg gtcaataacg ccgggatctt gtttacccag tgcaccgttg
    35101 aaaaccttac cgcagagcga atcaaccgag tactttccac caacgtgacg ggatattttc
    35161 tctgctgccg cgaggcggta aaacgcatgg cgcttaaaaa tggtggcagt ggcggcgcta
    35221 tcgtcaatgt ctcttcggtg gcctcacggt tgggttcgcc aggggaatat gttgattacg
    35281 cggcatcgaa aggggcgatt gatacgttaa ccaccggacg atcgctggaa gtcgccgcgc
    35341 aggggatccg cgttaactgc gtgcggccag ggtttattta taccgaaatg cacgccagcg
    35401 gcggcgagcc tggacgcgtc gatcgcgtta agtcgaacat ccccatgcag cgtggtggac
    35461 aggcagaaga ggtcgtgcag gccattgtct ggctactaag tgataaagcc tcttacgtca
    35521 cgggaagttt tatcgatttg gcgggcggga aataaaacag ggaagttgtc tgaccggatg
    35581 caacaagtat tgcatccggt acttcatcga cttaaagctt ctcgccgttg ctggcaatca
    35641 cctctttgta ccagttaaag ctcttcttac gtgaacgcga catatcgcca gtaccgtcgt
    35701 catgtttatt cacatagata aagccgtagc gtttgctgta ctgcccggta gtgaatgaca
    35761 cgcagtcgat gcagccccac ggtgtgtagc ccatcagatc cacaccatcg taagtcactg
    35821 cttttttcat ctcttcaata tgggcgcgca ggtagtcaat gcggtagtcg tcgttgatgc
    35881 tgccatcttc ttccactttg tcgtaagcgc caaaaccgtt ttcgacaata aacagcggcc
    35941 tctgataacg ctcatacagt tcgcaaaggg catagcgcag acctactgga tcaatctgcc
    36001 agccccagtc agacgcttta acatacgggt tcggtacgct gccttcaaaa ccagagatcg
    36061 catcgccggt gccgccttcg gccttcactg cgttggtcat gtaatagctg aaaccaagat
    36121 aatcgcaggt gccttcgcgc agtacatcga gatcgccgtc ttccattttg atattaaatc
    36181 cgcgacgttc ccactcgttc aatacatagg acgggtaata gccgcatagc tgcacatcgg
    36241 taaagacgta gcgttcgcgc atcgactcct gagcgaacat cacatcgtcc gggttacatg
    36301 agtagggata gagcggcacc atcgccagca tacagccgac tttcatctcc gggttaatgc
    36361 gacgcgcggc tttcactgcc agggcgctgg cgacaaactg gtgatgcagc acctggtaca
    36421 tcgtctcttc cgggttgtca tgctcggtat acaccacgcc ggagcagcag taaccaaaca
    36481 gcggcgcacg ccagttacgc tggttgttta tttcgttgaa ggtcatccag tatttgactt
    36541 tatgcttgta gcgttcgaat accacttccg cgaaacggac aaagaaatca acgaatttac
    36601 ggttggtcca gctaccgtat tgctgcacca gatgcagcgg catttcaaag tgggagaggg
    36661 ttatcaccgg ttcgatgttg tatttcagca gttcatcgaa cagagagtcg taaaacttca
    36721 gcccttcttc gtttggctga gcttcatcac cttttggaaa aatacgcgtc caggcgatgg
    36781 aagtccgaaa acatttgaag cccatttcgg caaatagctt gatgtcttcc ttgtagtgac
    36841 cataaaaatc aacggcttca tggtttggat agtattttcc tggcaagact tctttggtga
    36901 tttcgcgcgg cacgccgtgt gcgccaccgg tcagaacgtc acaaatgctc ggtcctttgc
    36961 cgcctttgtt ccagccgcct tcgacctgat gagcggcaac ggcgccaccc cataagaaat
    37021 ctttcggtaa ggtgagtttt ttcactatca taggctccag atcagtcaat tcttacagga
    37081 aatagtacca tgcagccaaa cgacatcact ttttttcaac gtttccagga tgacattctg
    37141 gctgggcgta aaaccatcac catccgcgac gagtctgaat cgcacttcaa aacgggtgat
    37201 gtgcttcgtg tcggacgttt tgaagatgac ggttattttt gcacgattga agtcaccgca
    37261 acctcaaccg taacgctgga tacactgacg gaaaaacatg cagagcagga aaatatgacc
    37321 ctgcctgaac tgaaaaaggt cattgctgac atctatcccg gtcagacaca attttatatg
    37381 attgaattta aatgtcttta aattgaactc acgcctgtta gttgaaattt cgcatttaat
    37441 ttcatgattt ttaggagatt agtctcaaat cagaatttat tttagctaac aggtgttcac
    37501 tggaactatt ctcagttacg ctggagaggt atacatgtgc gtgtacgttt ctctggagta
    37561 agttatggtt cagaagcccc tcattaagca gggatattcg ctggcagagg aaattgccaa
    37621 cagcgtcagt cacggcattg ggctggtgtt tggtatcgtt gggctggtgt tgctactggt
    37681 tcaggcggtg gatctcaatg ccagcgccac agcgataacc agttacagcc tctatggcgg
    37741 cagtatgatc ctgctgttcc tcgcttcgac gctctatcac gccattcctc atcaacgggc
    37801 aaaaatgtgg ctgaagaaat ttgaccattg cgctatttac ctgttgattg ccggaaccta
    37861 cacgccgttt ttgctggtgg ggctggattc tccgttagcg cgcgggttga tgattgttat
    37921 ctggagccta gcattgctgg ggattctgtt caaactgacc atcgcgcacc gattcaaaat
    37981 tttatctctg gtgacttatc tggcgatggg ctggctgtcg ctggtggtaa tttatgaaat
    38041 ggcagttaag ctcgcggcgg gcagcgttac cttactggcg gtaggcggcg tggtttattc
    38101 gctcggggtg attttctacg tctgcaaacg cattccatac aaccatgcca tctggcacgg
    38161 cttcgtgctc ggcggtagtg tgtgccactt tctggcgatc tatttgtata ttgggcaggc
    38221 gtaatgggta tgcctttgta ggcctgataa gacgcgtcag cgtcgcatca ggcatcggag
    38281 cgcaaccgcc ggatgcggcg tgaacgcctt atccgatcta cgggcctcgt ccctgttgtg
    38341 ataaaggcat caaatcccag acatgataag acgcgtcagc gtcgcatcag gcgataaatg
    38401 cctgatgcga tacagagatt tactcttcga gcgaatacgg cagcggctcg atatgcagtg
    38461 tattcgcatc gtcgcgtacg cggaagatgc tgtccggttc catatcgtta ttcatgacga
    38521 cctgtaccac gacctgacca tcttccagtt ttaccgcagc cagcaccgta ccggtacgac
    38581 gccagttctc gcccattttc agctctaagt cttcaccagc ttccggcagt cggctggcac
    38641 tacctgccag caaccagagc gcgcgtttgt tggcaccacg gaattttgct cgcgccacca
    38701 tctcttgtcc ggtataacag cctttcttaa agctgatacc gcccagcgcc tggagattgg
    38761 tcgcctgtgg gataaactgc ccgctgttgg cggcatcaat caccgggaaa ccagcttcaa
    38821 tgtttaatgc cagccactgt tggctattgt tcagttccgc ttcaccgcgc agtttatcgg
    38881 tcagcatatt agcagtagct tcatcggtta cgatcaggaa acgttctgcc gggtgttcaa
    38941 accatagcaa agtggtcgcg ccttctttga ctacctgttt ttctttcgaa ggcagttcgc
    39001 taaagagatt tgccagcgcg gcgcgcgcct gaaaaccggc aacaccaagc agcacacgct
    39061 cgtcgtctgg cgcgatggtc actttagaga ataccgcata ttttttcagt tcagtcagct
    39121 gcggttcacg cacgctgcgc cgttcaatcc atgcaaagcc atcgccgtcg cggaacagac
    39181 gtaaattgct ccacatttta cctttggcgt cgcaatgggc ggcgagcagg tgctgatctt
    39241 ctgccatctg gctgacatct gctgtcacct gaccctgcat atatttttcg ctgtccgcgc
    39301 cagtaatggt ggcaagcgcc cagtcatcaa gcgtcatcag cgtcagcggt aaacgggcag
    39361 aagccgtagg ctgacgtgga ggaaaaggtg taaaagccat aatagagtcc tgagttgctt
    39421 aacggcgtat taatgactaa tggtaaaaga gctatgaggc aatgcaagtg ctttcactac
    39481 gcgatctgtg ccttctgcga ggcgactgcc aaaaaaatga agcccttgaa ttgttgtgaa
    39541 ttaatttcgc gatcgcgatg gtgtttactg atattcccgt aaaagaagtc gaaaaacacg
    39601 ttacaatagc cgggtactgg attttcgtga gacaggaaga agatggacat taacaacaaa
    39661 gcccgcattc attgggcatg ccgccgtggt atgcgcgaac tcgatatttc aatcatgccg
    39721 tttttcgaac atgagtacga cagcttaagc gatgacgaaa aacgcatctt tattcgtctg
    39781 ctggaatgtg acgatccgga cctgtttaac tggctgatga atcacggtaa accggccgat
    39841 gcagaactgg aaataatggt ccgactcatc cagacacgga accgggaacg tggtcctgtg
    39901 gcaatctgat ttgcgcgtct cctggcgcgc acagtggctt tccttgctga ttcatgggct
    39961 ggttgccgct gttattttac tcatgccctg gccgctcagt tacaccccgt tatggatggt
    40021 gttactttcg ctggtggtat ttgattgcgt tcgcagccag cggtgtatta atgctcgcca
    40081 gggggaaatt cgcttgttga tggacgggcg tttgcgttgg caagggcagg agtggagcat
    40141 cgtcaaagca ccgtggatga ttaagagcgg catgatgctg cgtttacgtt ctgatggcgg
    40201 taaacggcaa catttatggc tggcagccga cagcatggac gaagccgaat ggcgggattt
    40261 acggcggatt ttgttgcaac aagagacgca aagataaaga tgacggcaaa gcatatgcgc
    40321 taagccgtca gtgagatcag gcgtaatgct ctgccatttc gttgaggatt tgctcgcacc
    40381 agctctgaat acgctcgtcg ctaaggtcat actggttagt ttcatccagc gccagaccca
    40441 cgaacagttg cccgtcagca atcaccggtt tcgggctggt aaattcatat ccttccgttg
    40501 gccagtagcc gacgaacttc acgcctttgg tcgagagttt gtcatgcagc ataccgagcg
    40561 catcgaggaa ccactcgccg tatcccagtt gatcgccaag cccatacagc gcaataattt
    40621 taccttcaag gttcaggtcg tcgagctgat cccagacggc ttcccagtct tcttggattt
    40681 caccaaaatc ccaggtcggg atacccagaa tgagcacatc gtactgctcc attaatttcg
    40741 gggagtcgtc cttgaggtta tgtaaggtca ccagttctgg gccgataata tcgcggattt
    40801 tttctgccgc catttcggtg taacaggtgc tggaaccgta aaaaagaccc atattcataa
    40861 cgtaagcttc tcgattctgc tgtggctttt gcagctagtg tatcagaatc gctttagtca
    40921 cggcacaata aggcataatg gatcaaatga gtgaccataa ggggcgcaag tgaaacagga
    40981 tctggcacgc atcgagcagt ttcttgatgc tctgtggctg gaaaaaaatc tggctgaaaa
    41041 tacgttgaac gcttaccgtc gcgatctgtc aatgatggtg gagtggttgc atcaccgcgg
    41101 gttgacgctg gcgacggcgc aaagtgacga tttgcaggca ttactggcag aacggctgga
    41161 gggcgggtat aaagccacca gctcagcgcg tttgctgagt gcagtgcgcc gattgttcca
    41221 gtatctttat cgcgaaaagt ttcgtgaaga cgatcccagt gcgcatctcg cttcaccgaa
    41281 attgccccag cgtttgccaa aagatttaag cgaagcgcag gtcgaacgtt tattacaggc
    41341 gccattaatt gatcagccac tggagctacg cgataaagcc atgcttgaag tgttgtatgc
    41401 taccgggctg cgtgtctctg aactggtcgg gctgacaatg agtgatatca gcctgcgtca
    41461 gggcgtagtg cgggtcattg gtaaaggcaa caaagagcgc ctggtgccgt taggggaaga
    41521 ggcggtttac tggctggaaa cctatctgga acatgggcgt ccgtggctgc tgaatggtgt
    41581 gtcaattgac gtgttgtttc ccagccagcg tgcgcagcag atgacgcgac agaccttctg
    41641 gcaccgcatt aaacattatg ccgtgctggc gggtatcgac agcgaaaagc tgtcaccgca
    41701 tgtgttgcgt cacgcttttg ccactcattt attaaatcat ggtgcggatt tacgcgtggt
    41761 gcagatgcta ctgggccaca gcgatctttc caccacgcaa atttatacgc atgtcgctac
    41821 cgagcgtctg cggcaacttc atcaacagca tcacccgcgg gcgtgatgtc tgaaaagaac
    41881 gggaagattt atgaagaaag gttttatgtt gtttactttg ttagcggcgt tttcaggctt
    41941 tgctcaggct gatgacgcgg caattcaaca aacgttagcc aaaatgggca tcaaaagcag
    42001 cgatattcag cccgcgcccg tagctggcat gaagacagtt ctgactaaca gcggtgtgtt
    42061 gtacatcacc gatgatggta aacatatcat tcaggggcca atgtatgacg ttagtggcac
    42121 ggctccggtc aatgtcacca ataagatgct gttaaagcag ttgaatgcgc ttgaaaaaga
    42181 gatgatcgtt tataaagcgc cgcaggaaaa acacgtcatc accgtgttta ctgatattac
    42241 ctgtggttac tgccacaaac tgcatgagca aatggcagac tataacgcgc tggggatcac
    42301 cgtgcgttat cttgctttcc cgcgccaggg gctggacagc gatgcagaga aagaaatgaa
    42361 agctatctgg tgtgcgaaag ataaaaacaa agcgtttgat gatgtgatgg caggtaaaag
    42421 cgtcgcacca gccagctgcg acgtggatat tgccgaccat tacgcacttg gcgtccagct
    42481 tggcgttagc ggtactccgg cagttgtgct gagcaatggc acacttgttc cgggttacca
    42541 gccgccgaaa gacatgaaag aattcctcga cgaacaccaa aaaatgacca gcggtaaata
    42601 attcgcgtga aacaacagat acaacttcgt cgccgtgaag tcgatgaaac ggcagacttg
    42661 cccgctgaat tgcctccctt gctgcgccgt ttatacgcca gccggggagt acgcagtgcg
    42721 caagaactgg aacgcagtgt taaaggtatg ttgccctggc agcaactaag cggtgtcgaa
    42781 aaggccgttg agatccttta caacgctttt cgcgaaggaa cgcggattat tgtggtcggt
    42841 gatttcgacg ccgacggcgc gaccagcacg gctctaagcg tgctggcgat gcgctcgctt
    42901 ggttgcagca atatcgacta cctggtacca aaccgtttcg aagacggtta cggcttaagc
    42961 ccggaagtgg tcgatcaggc ccatgcccgt ggcgcgcagt taattgtcac ggtggataac
    43021 ggtatttcct cccatgcggg agttgagcac gctcgctcgt tgggcatccc ggttattgtt
    43081 accgatcacc atttgccggg cgacacatta cccgcagcgg aagcgatcat taaccctaac
    43141 ttgcgcgact gtaatttccc gtcgaaatca ctggcaggcg tgggtgtggc gttttatctg
    43201 atgctggcgc tgcgcacctt tttgcgcgat cagggctggt ttgatgagcg cggcatcgca
    43261 attcctaacc tggcagaact gctggatctg gtagcgctgg ggacagtagc ggacgtcgtg
    43321 ccgctggacg ctaataatcg cattctgacc tggcagggga tgagtcgcat ccgtgccgga
    43381 aagtgccgtc cagggattaa agcgctgctg gaagtggcaa atcgcgatgc acaaaaacta
    43441 gccgccagcg atttaggttt tgcgctggga ccgcgtctca atgctgccgg gcgactggat
    43501 gatatgtccg ttggtgtggc gctgttgctg tgcgacaaca tcggagaagc gcgcgtgctg
    43561 gcaaatgaac tcgatgcgct aaaccagacg cgaaaagaga tcgaacaagg aatgcaggtt
    43621 gaagccctga ccctgtgcga gaaactggag cgcagccgtg acacgctacc cggcgggctg
    43681 gcaatgtatc accccgaatg gcatcagggc gttgtcggta ttctggcttc gcgtatcaaa
    43741 gagcgttttc accgtccggt tatcgccttt gcgccagcag gtgacggtac gctgaaaggt
    43801 tccggtcgct ccattcaggg gctgcatatg cgtgatgcgc tggagcgatt agacacactc
    43861 taccccggca tgatgctcaa gtttggcggt catgcgatgg cggcgggttt gtcgctggaa
    43921 gaggataaat tcgaactctt tcaacaacgg tttggcgaac tggttactga gtggctggcc
    43981 ccttcgctat tgcaaggcga agtggtgtca gacggtccgt taagcccggc cgaaatgacc
    44041 atggaagtgg cgcagctgct gcgcgatgct ggcccgtggg ggcagatgtt cccggagccg
    44101 ctgtttgacg gtcatttccg tctgctgcaa cagcggctgg tgggcgaacg tcatttgaag
    44161 gtgatggtcg aaccggtcgg cggcggtcca ctgctggatg gtattgcttt taatgtcgat
    44221 accgctctct ggccggataa cggcgtgcgc gaagtgcaac tggcttataa gctcgatatc
    44281 aacgagtttc gcggcaaccg cagcctgcaa attatcatcg acaatatctg gccaatttag
    44341 cgtcatcttc tctataaaaa agagcgtgga ttgggtacaa tcccgctctt atcaccgcat
    44401 tttgactagc tcaataaaag aaatcagacc atgtttgaaa ttaatccggt aaataatcgc
    44461 attcaggacc tcacggaacg ctccgacgtt cttagggggt atctttgact acgacgccaa
    44521 gaaagagcgt ctggaagaag taaacgccga gctggaacag ccggatgtct ggaacgaacc
    44581 cgaacgcgca caggcgctgg gtaaagagcg ttcctccctc gaagccgttg tcgacaccct
    44641 cgaccaaatg aaacaggggc tggaagatgt ttctggtctg ctggaactgg ctgtagaagc
    44701 tgacgacgaa gaaaccttta acgaagccgt tgctgaactc gacgccctgg aagaaaaact
    44761 ggcgcagctt gagttccgcc gtatgttctc tggcgaatat gacagcgccg actgttacct
    44821 cgatattcag gcggggtctg gcggtacgga agcacaggac tgggcgagca tgcttgagcg
    44881 tatgtatctg cgctgggcag aatcgcgtgg tttcaaaact gaaatcatcg aagagtcgga
    44941 aggtgaagtg gcgggtatta aatccgtgac gatcaaaatc tccggcgatt acgcttacgg
    45001 ctggctgcgt acagaaactg gcgttcaccg cctggtgcgt aaaagcccgt ttgactccgg
    45061 cggtcgtcgc cacacgtcgt tcagctctgc gtttgtttat ccggaagttg atgatgatat
    45121 tgatatcgaa atcaacccgg cggatctgcg cattgacgtt tatcgcgcgt ccggcgcggg
    45181 cggtcagcac gttaaccgta ccgaatctgc ggtgcgtatt acccacatcc cgaccgggat
    45241 cgtgacccag tgccagaacg accgttccca gcacaagaac aaagatcagg ccatgaagca
    45301 gatgaaagcg aagctttatg aactggagat gcagaagaaa aatgccgaga aacaggcgat
    45361 ggaagataac aaatctgaca tcggctgggg cagccagatt cgttcttatg tccttgatga
    45421 ctcccgcatt aaagatctgc gcaccggggt agaaacccgc aacacgcagg ccgtgctgga
    45481 cggcagcctg gatcaattta tcgaagcaag tttgaaagca gggttatgag gaaccaacat
    45541 gtctgaacaa cacgcacagg gcgctgacgc ggtagtcgat cttaacaatg aactgaaaac
    45601 gcgtcgtgag aagctggcga acctgcgtga gcagggaatt gccttcccga acgatttccg
    45661 tcgcgatcat acctctgacc aattgcacgc agaattcgac ggtaaagaga acgaagaact
    45721 ggaagcgctg aacatcgaag tcgccgttgc tggccgcatg atgacccgcc gtattatggg
    45781 taaagcgtct ttcgttaccc tgcaggatgt gggtggccgc attcagctgt acgttgcccg
    45841 tgacgatctg ccggaaggcg tttacaacga gcagttcaaa aaatgggacc tcggcgacat
    45901 cctcggcgcg aaaggtaaac tgttcaaaac caaaaccggc gaactgtcta tccactgtac
    45961 cgagctgcgt ctgctgacca aagcactgcg tccgctgccg gataaattcc acggcttgca
    46021 ggatcaggaa gcgcgctatc gtcagcgtta tctggatctc atctctaacg atgaatcccg
    46081 taacaccttt aaagtacgct cgcagatcct ctctggtatt cgccagttca tggtgaaccg
    46141 tggctttatg gaagttgaaa cgccgatgat gcaggtgatc ccaggcggtg ccgctgcgcg
    46201 tccgtttatc acccaccata acgcgctgga tctcgacatg tacctgcgta tcgcgccgga
    46261 actgtacctc aagcgtctgg tggtcggtgg cttcgagcgt gtattcgaaa tcaaccgtaa
    46321 cttccgtaac gaaggtattt ccgtacgtca taacccagag ttcaccatga tggaactcta
    46381 catggcttac gcagattaca aagatctgat cgagctgacc gaatcgctgt tccgtactct
    46441 ggcacaggat attctcggta agacggaagt gacctacggc gacgtgacgc tggacttcgg
    46501 taaaccgttc gaaaaactga ccatgcgtga agcgatcaag aaatatcgcc cggaaaccga
    46561 catggcggat ctggataact tcgactctgc gaaagcgatt gctgaatcta tcggtatcca
    46621 cgttgagaag agctggggtc tgggccgtat cgttaccgag atcttcgaag aagtggcaga
    46681 agcacatctg attcagccga ccttcattac tgaatatccg gcagaagttt ctccgctggc
    46741 gcgtcgtaac gacattaacc cggaaatcac agaccgcttt gagttcttca tcggtggtcg
    46801 tgaaatcggt aacggcttta gcgagctgaa tgacgcggaa gatcaggcgc aacgcttcct
    46861 ggatcaggtt gccgcgaaag acgcaggtga cgacgaagcg atgttctacg atgaagatta
    46921 cgttaccgca ctggaacatg gcttaccgcc gacagcaggt ctggggattg gcatcgaccg
    46981 tatggtaatg ctgttcacca acagccatac catccgcgac gttattctgt tcccggcgat
    47041 gcgtccggtg aaataagcat tacgttatgc tcacaacccc ggcaaatgtc ggggtttttt
    47101 atttaagctg ggtaaatgca gataatagta ttctggcttc gcgatttgtc gcctgcatca
    47161 ccatccacgg actgaacgcc cacggcgtgg catcaatacc gcgtaataca tcagctaaat
    47221 cacaccattg ataatccatc acttcatcat cgttgatctg taacgcactg gtggtgcgtg
    47281 cggcaaatac cggacacact tcattttcca caatgccact cggatcggtg gcgcggtagc
    47341 gaaagtcagg atagatagat tcaggaggcg taatttccac gccaagctca taacggcaac
    47401 ggcggatcac cgcgtcttcg ctgctttctc ccagttgtgg gtgcccacaa accgagttag
    47461 tccacacgcc aggccacgct tttttgctca gtgcgcggcg ggtaactaat aattgcccct
    47521 tggcattaaa cagccaactg gaaaacgcga gatgtaagag ggtgtctgcc gtgtgtgcgg
    47581 catacttttc cagcgtaccc gtgggaactc cctgtgcatt caataaaatg acgtgttccg
    47641 tttgcataat ttctcacatg taattctgat cactcatcat tatagataat tgaagagtga
    47701 aatgattaac caagagagcg tttatcgaca accgcccgtt ttgagcggat gccgacaggt
    47761 aaaaaccatt taaaaaagta gcatcatata tttgaagcga ctttcaatgg ccattgcaac
    47821 acagtcagaa atgacaggaa tagagagagt aaaaagcaac aacagactga atatagcgcg
    47881 cattaaaaaa ttcatattat tctccatgct catttttctt cagcttgcgc atcagaagcc
    47941 acatacgtac tgtcctgact tttaaatctt tatcagagac ggattccttc accaggtcag
    48001 catgatgctg atagccatta aagaagacgt tcagcacaac ggcgctaagg gttgcgagca
    48061 taatgccgct atgtagcagc ggttgcagta cggccggtaa cttagaaaag aaatcgtgag
    48121 agagcgtcgg agtcatgcca acgccgagac tgatcgccac aatatagagg ttgtaacggt
    48181 tggtggtgta gttacagcgc gacagaattc gaatccctgt cgccagtacc atgccgaaca
    48241 tcactagacc agcgccgccc agcacaaatt gcggaatgga ggctaccagc accgccattt
    48301 ttggcaccat gccaaacagg attaatataa ttcccgaaga aatacacacc cagcggctat
    48361 gaacgcgcat cacgctaacc aggccaacgt tttgagaaaa tgacgtgtgg gggaagctgt
    48421 taaacgtgcc gcctatcatt gtccctacgc catcgacacg cagcccgcga ataatatcgt
    48481 gtgaagagag tttacgaccg actatttcac ccagtgccag gaacatcccc atcgactcga
    48541 taaacacgat gattaacacg gcagtcatgg tcagaatgga aacgggatcg aaaatcggca
    48601 taccaaacga catcggggtg acaatcgcaa accacgaggc atcatgtagc ccggataaat
    48661 tgacttcatt catcatccac gaaagtaaaa agccaaatac aatccccagt aatacggcga
    48721 cgttggacat aaatcctttc gcatagcgag taatgagcaa gataaaaatt aagacggcaa
    48781 aggagatacc taaataaacg ggattaccat attgcggatt ccctttacct ccagcggccc
    48841 agtcaatacc cacctgaatg atgctaagcc caatagaagt aataaccaca ccggtaacca
    48901 gtggcgggaa taaaggcatc aagcgaccaa taagtggcgc taataatgtg gtgataaaac
    48961 ccgcggcgat agtggcacca aatatcccca gcaggccgat atccgggttc atcccaatgg
    49021 ctatcattgg tgttacagca gcaaaggtca ccgacataat caccggcagg cggatcccca
    49081 taaagcggcc gataccgata cattgcaata atgtgacgat cccgcagcaa aagagatccg
    49141 agctaatgag catcgcaata gcatcttttg agaggcccag tcggtcacca atcataagag
    49201 gaacagcgac tgcacctgcg tacatgacaa ggacgtgttg cagaccgagt atgatcagct
    49261 ttcctggtga taatatgcgg tcaacctcat cagttgggcg gtcttgccca gaagatgagg
    49321 gaagttggga atctatggcg ctcatcagtg tttctccttt cgcccgccag aataaaaagc
    49381 aaccgtttac gttaatgaaa ctgtctaaac acatgctcgt ggagtgccag tttttgcgag
    49441 gaaggggagt tagagacagg aattgcggag gcaatcacat aatcgtgctt aaaccacgaa
    49501 aatgacaaga gaacctgata atggaattat ctgtaatttt ctcatattga tatttcatta
    49561 atcattctgg cat
//
LOCUS       DAETPZ010000023        48169 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.023, whole genome shotgun sequence.
ACCESSION   DAETPZ010000023 DAETPZ010000000
VERSION     DAETPZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48169
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.023"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..164
                     /locus_tag="K0W59_001483"
     CDS             <1..164
                     /locus_tag="K0W59_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022581.1"
                     /translation="ALTAIVGFAGMIITWIVKSFDEDVDYYVPVAEIEKLENQHFDEI
                     TKAGLKNGN"
     gene            154..768
                     /locus_tag="K0W59_001484"
     CDS             154..768
                     /locus_tag="K0W59_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HBI3022582.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            768..1097
                     /locus_tag="K0W59_001485"
     CDS             768..1097
                     /locus_tag="K0W59_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HBI3022583.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            1109..1999
                     /gene="cyoE"
                     /locus_tag="K0W59_001486"
     CDS             1109..1999
                     /gene="cyoE"
                     /locus_tag="K0W59_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HBI3022584.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FSANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            2184..2495
                     /locus_tag="K0W59_001487"
     CDS             2184..2495
                     /locus_tag="K0W59_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBI3022585.1"
                     /translation="MLGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            2479..3021
                     /locus_tag="K0W59_001488"
     CDS             2479..3021
                     /locus_tag="K0W59_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI3022586.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVM
                     LGGARKYQKRGFGQDLLCDFFEHVKKIHQALPIKGVYLDADPATINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(3077..4012)
                     /locus_tag="K0W59_001489"
     CDS             complement(3077..4012)
                     /locus_tag="K0W59_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBI3022587.1"
                     /translation="MLPNRILFSLMLIVSSGVVASKKAHELYDSIYGGKSAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGSIYYD
                     GEQVRVDYAKAYALFNQAAQQGMNLAWSRLGMMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVEDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            complement(4023..4256)
                     /locus_tag="K0W59_001490"
     CDS             complement(4023..4256)
                     /locus_tag="K0W59_001490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022588.1"
                     /translation="MSKEYTLSVLPFSLNTLGVDMYNNSSYIRKSYIDIPMYNRNLCD
                     FHDLTYSIKSATAILNGKQRDLLKEEHIQVQDK"
     gene            complement(4249..5525)
                     /locus_tag="K0W59_001491"
                     /pseudo
     CDS             complement(4249..5525)
                     /locus_tag="K0W59_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105027.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            5919..7289
                     /locus_tag="K0W59_001492"
     CDS             5919..7289
                     /locus_tag="K0W59_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3022589.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFIVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAITAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQRVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPENVMADEKLEKCLLTTPGVKDVLLAKLEHCVYVKVDSKITNRFEIERV
                     INEVSY"
     gene            7471..9625
                     /locus_tag="K0W59_001493"
                     /pseudo
     CDS             7471..9625
                     /locus_tag="K0W59_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
     gene            9678..11408
                     /locus_tag="K0W59_001494"
     CDS             9678..11408
                     /locus_tag="K0W59_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022590.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSDPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDEIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQYVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(11468..11959)
                     /gene="yajQ"
                     /locus_tag="K0W59_001495"
     CDS             complement(11468..11959)
                     /gene="yajQ"
                     /locus_tag="K0W59_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HBI3022591.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            12127..13038
                     /gene="panE"
                     /locus_tag="K0W59_001496"
     CDS             12127..13038
                     /gene="panE"
                     /locus_tag="K0W59_001496"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBI3022592.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARTHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            13001..13591
                     /gene="yajL"
                     /locus_tag="K0W59_001497"
     CDS             13001..13591
                     /gene="yajL"
                     /locus_tag="K0W59_001497"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HBI3022593.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDAQVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(13645..15093)
                     /gene="thiI"
                     /locus_tag="K0W59_001498"
     CDS             complement(13645..15093)
                     /gene="thiI"
                     /locus_tag="K0W59_001498"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBI3022594.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            15299..15541
                     /gene="xseB"
                     /locus_tag="K0W59_001499"
     CDS             15299..15541
                     /gene="xseB"
                     /locus_tag="K0W59_001499"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBI3022595.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            15541..16440
                     /gene="ispA"
                     /locus_tag="K0W59_001500"
     CDS             15541..16440
                     /gene="ispA"
                     /locus_tag="K0W59_001500"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HBI3022596.1"
                     /translation="MDFPQQFEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            16465..18327
                     /gene="dxs"
                     /locus_tag="K0W59_001501"
     CDS             16465..18327
                     /gene="dxs"
                     /locus_tag="K0W59_001501"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBI3022597.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTICVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            18382..19356
                     /gene="yajO"
                     /locus_tag="K0W59_001502"
     CDS             18382..19356
                     /gene="yajO"
                     /locus_tag="K0W59_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HBI3022598.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRGDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPMARGR
                     LTRPWGETTARLVSDDVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(19410..19928)
                     /gene="pgpA"
                     /locus_tag="K0W59_001503"
     CDS             complement(19410..19928)
                     /gene="pgpA"
                     /locus_tag="K0W59_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBI3022599.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(19906..20883)
                     /gene="thiL"
                     /locus_tag="K0W59_001504"
     CDS             complement(19906..20883)
                     /gene="thiL"
                     /locus_tag="K0W59_001504"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HBI3022600.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(20961..21380)
                     /gene="nusB"
                     /locus_tag="K0W59_001505"
     CDS             complement(20961..21380)
                     /gene="nusB"
                     /locus_tag="K0W59_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBI3022601.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(21400..21870)
                     /gene="ribE"
                     /locus_tag="K0W59_001506"
     CDS             complement(21400..21870)
                     /gene="ribE"
                     /locus_tag="K0W59_001506"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBI3022602.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(21962..23062)
                     /gene="ribD"
                     /locus_tag="K0W59_001507"
                     /pseudo
     CDS             complement(21962..23062)
                     /gene="ribD"
                     /locus_tag="K0W59_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
     gene            complement(23066..23515)
                     /gene="nrdR"
                     /locus_tag="K0W59_001508"
     CDS             complement(23066..23515)
                     /gene="nrdR"
                     /locus_tag="K0W59_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HBI3022603.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            23666..24205
                     /locus_tag="K0W59_001509"
     CDS             23666..24205
                     /locus_tag="K0W59_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBI3022604.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            24504..25388
                     /gene="tsx"
                     /locus_tag="K0W59_001510"
     CDS             24504..25388
                     /gene="tsx"
                     /locus_tag="K0W59_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HBI3022605.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKIRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(25565..25912)
                     /locus_tag="K0W59_001511"
     CDS             complement(25565..25912)
                     /locus_tag="K0W59_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HBI3022606.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(26041..27012)
                     /gene="secF"
                     /locus_tag="K0W59_001512"
     CDS             complement(26041..27012)
                     /gene="secF"
                     /locus_tag="K0W59_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HBI3022607.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(27023..28870)
                     /gene="secD"
                     /locus_tag="K0W59_001513"
     CDS             complement(27023..28870)
                     /gene="secD"
                     /locus_tag="K0W59_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HBI3022608.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(28898..29230)
                     /gene="yajC"
                     /locus_tag="K0W59_001514"
     CDS             complement(28898..29230)
                     /gene="yajC"
                     /locus_tag="K0W59_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBI3022609.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(29253..30380)
                     /gene="tgt"
                     /locus_tag="K0W59_001515"
     CDS             complement(29253..30380)
                     /gene="tgt"
                     /locus_tag="K0W59_001515"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBI3022610.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(30435..31505)
                     /gene="queA"
                     /locus_tag="K0W59_001516"
     CDS             complement(30435..31505)
                     /gene="queA"
                     /locus_tag="K0W59_001516"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBI3022611.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            31598..32171
                     /gene="acpH"
                     /locus_tag="K0W59_001517"
                     /pseudo
     CDS             31598..32171
                     /gene="acpH"
                     /locus_tag="K0W59_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(32176..33993)
                     /gene="malZ"
                     /locus_tag="K0W59_001518"
     CDS             complement(32176..33993)
                     /gene="malZ"
                     /locus_tag="K0W59_001518"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HBI3022612.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYLFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQSEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPSCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(34149..35522)
                     /gene="proY"
                     /locus_tag="K0W59_001519"
     CDS             complement(34149..35522)
                     /gene="proY"
                     /locus_tag="K0W59_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HBI3022613.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKIAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(35598..36917)
                     /gene="brnQ"
                     /locus_tag="K0W59_001520"
     CDS             complement(35598..36917)
                     /gene="brnQ"
                     /locus_tag="K0W59_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HBI3022614.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(37324..38619)
                     /gene="phoR"
                     /locus_tag="K0W59_001521"
     CDS             complement(37324..38619)
                     /gene="phoR"
                     /locus_tag="K0W59_001521"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HBI3022615.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEASPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(38677..39366)
                     /gene="phoB"
                     /locus_tag="K0W59_001522"
     CDS             complement(38677..39366)
                     /gene="phoB"
                     /locus_tag="K0W59_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HBI3022616.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLLGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            39556..40758
                     /gene="sbcD"
                     /locus_tag="K0W59_001523"
     CDS             39556..40758
                     /gene="sbcD"
                     /locus_tag="K0W59_001523"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HBI3022617.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            40755..43898
                     /gene="sbcC"
                     /locus_tag="K0W59_001524"
     CDS             40755..43898
                     /gene="sbcC"
                     /locus_tag="K0W59_001524"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HBI3022618.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADMKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVELDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTTLQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            44024..45205
                     /gene="araJ"
                     /locus_tag="K0W59_001525"
     CDS             44024..45205
                     /gene="araJ"
                     /locus_tag="K0W59_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HBI3022619.1"
                     /translation="MKKVILSLALGTFGLGIAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALFSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(45348..46256)
                     /gene="mak"
                     /locus_tag="K0W59_001526"
     CDS             complement(45348..46256)
                     /gene="mak"
                     /locus_tag="K0W59_001526"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HBI3022620.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            46381..47292
                     /gene="rdgC"
                     /locus_tag="K0W59_001527"
     CDS             46381..47292
                     /gene="rdgC"
                     /locus_tag="K0W59_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HBI3022621.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELHDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(47370..47825)
                     /locus_tag="K0W59_001528"
     CDS             complement(47370..47825)
                     /locus_tag="K0W59_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022622.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAAGRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(47822..>48169)
                     /locus_tag="K0W59_001529"
     CDS             complement(47822..>48169)
                     /locus_tag="K0W59_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HBI3022623.1"
                     /translation="LQVFFAELATIKDKSDDEWWRALLQSRNDHLRQTALRNAHTPAS
                     LLTTLTEPQDRSLAINNPQLAADVKTAWLKEDPSLLLFVEQPDLSQLRDLVKTGATRK
                     IRSEARHRLEEKQ"
BASE COUNT        12472 a        12365 c        11697 g        11635 t
ORIGIN      
        1 gggcgcttac ggcgattgtt ggcttcgcag gcatgatcat cacctggatc gtgaaaagct
       61 tcgacgagga cgtggattac tacgtgccgg tggcagaaat cgaaaaactg gaaaaccagc
      121 atttcgatga gattactaag gcagggctga aaaatggcaa ctgatacttt gacgcacgcg
      181 actgcccacg cgcacgaaca cgggcaccac gatgcaggcg gaaccaaaat cttcggattt
      241 tggatctacc tgatgagcga ctgcattctg ttctcaatct tgtttgctac ctatgccgtt
      301 ctggtgaacg gcaccgcagg cggcccgaca ggtaaggaca ttttcgaact gccgttcgtt
      361 ctggttgaaa ctttcttgct gttgttcagc tccatcacct acggcatggc ggctatcgcc
      421 atgtacaaaa acaacaaaag ccaggttatc tcctggctgg cgttgacctg gttgtttggt
      481 gccggattta tcgggatgga aatctatgaa ttccatcacc tgattgttaa cggcatgggt
      541 ccggatcgca gcggcttcct gtcagcgttc tttgcgctgg tcggcacgca cggtctgcac
      601 gtcacttctg gtcttatctg gatggcggtg ctgatggtgc aaatcgcccg tcgcggcctg
      661 accagcacta accgtacccg catcatgtgc ctgagcctgt tctggcactt cctggatgtg
      721 gtttggatct gtgtgttcac tgttgtttat ctgatggggg cgatgtaatg agtcattcta
      781 ccgatcacag cggcgcgtcc catggcagcg taaaaaccta catgacaggc tttatcctgt
      841 cgatcattct gacggtgatt ccgttctgga tggtgatgac aggggctgcc tctccggccg
      901 taattctggg aacaatcctg gcaatggcag tggtacaggt tctggtgcat ctggtgtgct
      961 tcctgcacat gaataccaaa tcagatgaag gctggaacat gacggcgttt gtcttcaccg
     1021 tgctaatcat cgctatcctg gttgtaggct ccatctggat tatgtggaac ctcaactaca
     1081 acatgatgat gcactaagag cggcggttat gatgtttaag caatacctgc aagtaacgaa
     1141 accaggcatc atctttggca acctgatctc ggtgattggg ggattcctgc tggcctcaaa
     1201 gggcagcatt gattatcccc tgtttatcta cacgctggtt ggggtgtcac tggttgtggc
     1261 gtcgggttgt gtgtttaaca actacatcga cagggatatc gacagaaaga tggaaaggac
     1321 gaagaatcgg gtgctggtta aaggcctgat ctctcctgct gtctcgctgg tgtacgccac
     1381 cttgctgggt attgctggct ttatgctgct gtggtttagc gcgaatccgc tggcctgctg
     1441 gctgggggtg atgggctttg tggtttatgt cggcgtctat agcctgtaca tgaaacgcca
     1501 ctcagtctac ggcacattga ttggttcgct ctccggcgct gcgccgccgg tgattggcta
     1561 ctgtgcagta accggcgagt ttgatagcgg cgcagcgatc ctgctggcta tcttcagcct
     1621 gtggcagatg cctcactcct atgccatcgc cattttccgc tttaaggatt accaggcggc
     1681 aaacattccg gtattgccag tggtaaaagg catttcggtg gcgaagaatc acatcacgct
     1741 gtatatcatc gcctttgccg ttgccacgct gatgctctct cttggcggtt acgctgggta
     1801 taaatatctg gtggtcgccg cggcggttag cgtctggtgg ttaggtatgg ctctgcgcgg
     1861 ttataaagtt gctgatgaca gaatctgggc gcgcaagctg ttcggcttct ctatcatcgc
     1921 catcactgcc ctctcggtga tgatgtccgt tgattttatg gtaccggact cgcatacgct
     1981 gctggctgct gtgtggtaac aaaacctctc tattaaaaag gtgctacggc acctttttta
     2041 ttgtctgaga tcagagatac ggctaataac cgtatctctt gacagagtga gcagatgcac
     2101 ataaattaat catacggttg atagccgtat tatgtatgag atcgactccc tacgatttca
     2161 ttcagttaat caaagaagtc ataatgcttg gaaaaacagc cgctctatac gatgtcgata
     2221 aaacgctgaa aaatgcgcgg gtcgaactca aaactagtcc cgacgccaaa aacaaacttc
     2281 gtgaagctgc gcaagccgtt ggggtagatt taagcgcttt tattttgagc gctgctatgg
     2341 aacgcgccga aagtgtgctt gataaccaac gtcgccgtga gctttcgaat caaagctggg
     2401 aactgatgaa ccaactcatc gctgaacctg ctcaaccgac gctcgccctc aaggcgttaa
     2461 tgaaaaggaa aaacagcgat ggtcgacaag catgaagaga ttactctgcc catagtcctc
     2521 tcctgtaatt atcagtctga tattacttat cctgggcaaa aacagtttga ttgcggtaac
     2581 cctgttatcg ataaatttgt acgcgcatcg ctaaagaaaa gtgtgcgtaa tagcgactgt
     2641 gcggctaaag cacttattga cagacaaagt ggtgaactga ttggcatctg tacttttacg
     2701 gcatattcgc tggaaagaca acgcgtttct ggcgtccttc agggttcaca accttcagaa
     2761 attggtgttg tcagattagt catgttgggg ggagcgcgga agtatcaaaa gcggggcttt
     2821 ggtcaggacc tactatgtga tttttttgaa catgtaaaaa aaattcacca ggcattacca
     2881 attaaagggg tttatcttga tgctgaccct gccaccatta acttttatgc tcgtctcggc
     2941 tttgttcagc tttcagcgac accaaatgct tttggtgctg tacctatgtt tttggcgatt
     3001 cagcatattc tcgcggctta gattattatc gccacccgcc agggcaagtt ttgccttagc
     3061 cgggggctga gcagcgttac cacttttgtt caattgcccc cgctttcagc aactccacat
     3121 ccttgccgtt aatagttgcc gttgcggcaa cgattgtggt ccttacagtt gcacaagatt
     3181 ttcgctcaaa ttccggttta taaagaataa gagacgaatt actggtaaat gaaccaaata
     3241 ggtaatcagt aaagctaata ttcagccggt ttaaaccgaa aggcgcgaag ccaaccgttt
     3301 ggttaatctc cttaccggat gggcgacgaa tgctgaatgt cacgcgaaga ttggccactt
     3361 cgcctaattt attggtggtg gcgaagaggc tatccatgca ggaaaaacct ttatctaaaa
     3421 aattatcccg catcccagaa cgggtaaccg tgatcacggg taacgtatcc tcaaccgttt
     3481 ttctgtcaat catgtctttt cgacaagtag ccagaaagtc ttccgggcca ccataaatgt
     3541 ggacaccttt atcaaggtat tccttcgctt tcttacaatc tacctcaaca tactgaccat
     3601 tggcgtacat catgcccaac ctggaccatg ctaaattcat accttgctgc gcggcctgat
     3661 taaatagtgc atacgctttg gcataatcca cccgcacctg ctcaccgtcg tagtagatac
     3721 tccccagcgc taacggagct tctctgtcac cttgtttagc ggctaactcg aaccatttaa
     3781 tcgcttcctg aacatcaggt ttggtatcat agcgaggctg ataatattcc cagcctaaca
     3841 ggctctgcgc gtcgatatct cccgattcag ccattttatg cagtgtattt ataacatcgg
     3901 gagcagattt accaccgtat atgctgtcat acaattcatg cgctttctta cttgccacca
     3961 cgcctgatga aactatcagc attaaagaaa ataatattct attaggcaac ataaaatatc
     4021 tcttatttat cttgtacctg aatatgctct tcttttaata aatcacgttg ttttccattc
     4081 agtatcgctg tcgcagattt aatagaataa gtcaaatcat gaaaatcgca taaattacga
     4141 ttatacattg ggatgtctat atagcttttt cttatataac tggaattatt atacatatcc
     4201 acaccgagcg tattcaaact aaaaggaagg acagaaagag tatattcttt actcactccc
     4261 tccccccccc ccttttagct caagcgtcaa ctgcaaatta gttatactac taattttatc
     4321 tgcattaatt tcaatactga catcgcatat tttggttttt tcatttgcgt taaagccata
     4381 ggtcccgtaa tgttttaacg tgatgtaagg taaagtatca ccctgtgttt ctcgggtttt
     4441 ctgatcttga ttacaacggg ttaaatattc atccttattg aatttcattg aatatttccc
     4501 cgcacctttt tcgtaataat cccatacctt atcacaatca agcgtttcac ctaatccgtt
     4561 gtaatacatc caacttaacc acaatgcggc ttttttgccc cttgctgttc agccttgtca
     4621 aaaagcatgc tcgctttaga ataatcaacc tcaccggtca aaccagaata gtatatattc
     4681 cccagggcca ctaatgcttt ttcgtcattc ttatttgcag ctaattcgta ccattttttg
     4741 gcttcgacag catcaatctt taaaccagca ctgccatttt cgtaggtatc gcccatggta
     4801 tactgcgcca tcggataacc tttttctgcc gcagtacgaa accattgcag tgctttcttt
     4861 atggtgtcta aagaacgtct atcatcagaa ggtgaaaaat catcgagata aagatttccg
     4921 acaatataat ccatttcggg gtcgccttcc tgcgcccata gagtcgccag acgcagaatt
     4981 tcaggtttac tgaaaccaat tcgcccccaa aaaagcaaat cattctcggg tgcctgaccg
     5041 acataacgac tgacaaattc aattaaactc gcccgtttcg ttttgtctgt ctcagccccg
     5101 gatttcaatt cataggcctg attgattatt ttattcatca aatcaggcgg aatattaaga
     5161 tcgtcagttt tcgacttact ggcatacttc tgtgctcgtg tcacatctag ggtgacgcct
     5221 ttaccatatt gaaagaaata acgtgcatta taacaaccgt attcgccccc ctggttacag
     5281 gctttctcat accactgaat cgcctttggg atgtcaacag atactgtcac tccgtgttca
     5341 taaataaatc ccagtagcac tagcgcctgc gtgttattgg tatcagctaa ttttttcagt
     5401 tcttttaacg ccgtctgatc tttattgaca atacgttcat acagttgatc aatatctgtt
     5461 gaagcatgac aaaaagaagc cagagataaa cccaatgata ataaaataac tttaccagac
     5521 cacatacatt cctccttgat atattcaacc accaaacatc cacctcatga ataaatacgc
     5581 tgccataacg gcccaggtaa taagtaaaca tttaagctga aacagcataa ccagtgcctc
     5641 caacgcttct atttattgcg ataaaataac accatccttt gataaaaagt cacacgccca
     5701 tcaataaatc caaaaataat gtcattagtt gcaggataaa atcatttgag cttttcattt
     5761 gttatattta acgcatgatg tattaatggc aagaatatga gagaaactca tacctgacgt
     5821 aaaaatcact ctcttatgta atatttactt attaacactc tgtttaccta acatgcgctc
     5881 cgcactacac tatgtgggca attttttaag aggtggtaat gaacgattat aaaatgacgc
     5941 caggtgagcg gcgcgcgacc tggggtttag ggaccgtatt ttcattgcgc atgctgggca
     6001 tgtttattgt actgccggtt ctgactacgt acggtatggc actgcaaggc gccagcgaag
     6061 cattaatcgg tattgccatt ggtatttatg gtctgaccca ggccgttttt cagattccgt
     6121 ttggcctgct ttccgaccgc attggtcgaa aaccattaat tgtcggtggg ctggcagtgt
     6181 ttgccgccgg tagcgttatc gctgcgcttt ctgactccat ctggggaatt attctgggcc
     6241 gggcgctaca aggctccggt gcgattaccg ctgccgttat ggcgctgctt tccgatctca
     6301 cgcgcgaaca aaaccgtacc aaagcaatgg cgtttatcgg cgtgagcttt ggcattacct
     6361 ttgccattgc gatggtgctt ggcccgatca tcactcacaa acttgggctg cacgcgctgt
     6421 tctggatgat cgctattctg gcaacgaccg gcattgcgtt gaccatttgg gttgtgccca
     6481 acagtagcac tcacgtactt aatcgtgagt ccggaatggt gaaaggcagt ttcagtaaag
     6541 tgctggcgga accgcggctg ctgaaactca actttggcat tatgtgtctg catattttgc
     6601 tgatgtcgac gtttgttgcc ctgcccggac aactggctga tgcggggttc ccggcggctg
     6661 aacactggaa ggtctatctg gcgacaatgc taatcgcctt tggctcggtc gtgcctttca
     6721 ttatctacgc tgaagttaag cgcaaaatga agcaagtctt tgtcttctgc gtcgggttga
     6781 tcgtggttgc ggaaattgtg ttgtggaacg cgcaaacgca gttctggcaa ctggtggtcg
     6841 gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc cctgctgccc tcacttatca
     6901 gtaaagagtc gccagcaggt tacaaaggta cggcgatggg tgtttactcc accagccagt
     6961 ttcttggcgt ggcgattggc ggttcgctgg gcggctggat tgacggcatg tttgacggtc
     7021 agcgggtatt tctcgctggc gcaatgctgg ccgcagtgtg gctggcagtc gccagtacca
     7081 tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat cccagaaaac gtaatggcag
     7141 atgaaaaact ggaaaaatgc ttactgacaa cgcctggtgt caaagatgta ctgctggcga
     7201 agttagaaca ttgcgtctat gtgaaagtag acagtaaaat caccaatcgt tttgaaatag
     7261 agcgagtaat caacgaagta tcttattaaa taataagcac ggtgctattc atgaaaaata
     7321 aaatagcacc gtacttcgta acaccataat cctttaaacc ctccattatt aatacttcgc
     7381 ttaattccat ataactggac gattacatta gaagtaaact atacttttta tgaaatatca
     7441 gggataaatt attcacatgg aaaatacatc atgtacaaat cattatttac tattatcatt
     7501 ctactgtata gcgccagttc atatgcaaaa agcgaatccc ctcccgcaag cactataact
     7561 ttcattgcac aaatcgatca tgttgagcta aataaaacag cattatctca tgatatgaaa
     7621 ctcgttcttg ccgatcgttt ttatttcaaa accagtaatc cttgcgaaac aaattcaatt
     7681 tctgctcgac cagaatcaat ggatattact gacaaagcct atgccaacaa attaaaaaaa
     7741 ttagaaactc cttttcttga tgatcgctat ttctttatga ccatcaatca gtgtgacggt
     7801 aatggcactc ccatgctgac agacataaaa gtatgtacaa cagaattatg tggtgctgaa
     7861 tatacaaaga aaatgtctac tgtatggctc gatgaaactt taaaaccaac tgctaaacgt
     7921 ctggcaattt attttattca atctccttta ccctatgacg aggagaaaaa acaatggaaa
     7981 gtcaccggat ggtatatctc atcaactgat aatcagtctt caactaaaca gattgcattt
     8041 gaaggatata cagacacaga taacttctcc gattctaaat ttatttcttc atttaaatcc
     8101 tggtttgaaa gtggaaagat aaaaaacatt gctaactata atccaaaagg agaaagagat
     8161 ggaaaatacg aaagttatta caatagcaat ggtaacgtct atgaatctct aagtttttct
     8221 aatggtctta tcaacggtga gtttattaca tattatgaaa atggggccat tgaaagtaaa
     8281 cgtcatttta ataacgacaa aattaaagat ggtgaatgcc ctcattttta tgaagatggg
     8341 aaaatcaaac agcaacatag ctatctgaat agtaaactcg atggtcctgc atatgaatat
     8401 tatcctgatg gcaaactcat gcaggaaaat ttctatcagc aaagtgtgtt gattggaaac
     8461 gataccagtt actaccaatc cggaaaaata agctctatac acaatagaaa ttcccgtggt
     8521 cagtatgatg gagctaacga acgttatagt gaagagggta agttacagtc taaatccgtc
     8581 tataaagacg gaaaacaaat ttctgttcaa acctggtatg aaaatgggca gaaagaagag
     8641 gagaaacatt ttgatgagca aggccaatta aatagtctgg ttaagcaatg gtataaaaat
     8701 ggtaatcttg caaaatcaca aaactataaa catgacatac tcgatggtaa cagtgaagag
     8761 tggtacgaaa atggcattcc tgaaagtttg tacccttata agaatggaaa aaccgacggc
     8821 attgccaaat catggaataa gtatggtaaa ttaacctatt caatcgaata caaaaatggc
     8881 gtagagaatg gggcatatcg taactggagc aaaaacacag gtaaactaac caaagaaacc
     8941 ctgtacgtca atggtatcag acagggagtg gaaaaagagt ttaacgatcg cacaggtaaa
     9001 ctattaacct ccacgcaata tgttaataat aaaagacatg gtacggagga aacttatgat
     9061 caaaacggaa taaaatatat tacatgctat caaaatgatc agaaattatc gtctttagac
     9121 aatccaacac aaataaaaga taatgcaaca acaggcgatt ccagtgctca atttgcatta
     9181 gggaaatacg aattcatctg tgcaaatata gatgaaggga ttaaatggtt aaccaaatct
     9241 gcagagcaaa aaaaacactg atgcgatata ctttttagct acagcctaca aaggcaatgg
     9301 tatacctgca aacaatgaaa aatacctagc atatctgcaa caagctgcaa cactgggcag
     9361 tagtaatgcc caggcggaaa ttggctattt atatctcatc ggaaaagaac taccacagaa
     9421 tcttcctgat gctggtgtct ggttcaaaaa agcagctgca caaggtaatt ttgccgcaca
     9481 tttctatctg ggaaggatgt atcagaatgg tgacggcgtt gagagaaata tggaaaaagc
     9541 caggtttcat ctatctaatg cggcagaagg tggaatagaa cctgcgctta aagcattaaa
     9601 tgaacttgaa catcaaacaa aataaagcgc tcagggacca cgtaaggttc atcaaacaga
     9661 aaaggatata gtttgcgatg aaagcgaaat ctactttgtt attaatgatt ttatctgttt
     9721 tttattctca tcaggtttta tccagcgacc ccactcagcc ggatgtttat tctgtcgtcg
     9781 agaaaaaact aaactctgct ttacctctgg aagaaaatac caccttcaga tcgcaggctg
     9841 aatggtttaa tttgcaatat gaattattag tatctggcta ccctgaacgt gcttacaatt
     9901 tactgagcaa acttgagaca gaatcgaaaa acgctaaata ttatctggat ttaactgcct
     9961 ccagagtcgc tgaagaagaa gcaccagaga cagccttaac ttttttatca aaaattgggt
    10021 taaataaacc ggctactctt acatcctttg agagctatat caatagctgg ataaaaagca
    10081 aacaacccga aaaggctatg cagcttctca gcctcgatga gaatgccctg aattattatt
    10141 taccgactgt gttagtcgca tatcagatcg ttcctgataa ggcaataagt atttataatt
    10201 ctgtatatgg agataatgtt gttataccct atcagcaagt caccatgtta cttacagttg
    10261 cggagggtta tcaaacaaaa ggcgatataa aaaacgccat tcattatgcc gataaggcgc
    10321 tgaatatgtt tgataaagct attgctgtag atagcagtcg tgaatcccat tatagtgatg
    10381 agtatttgaa actcatgaat atctacgccg ccaatggcaa caaagaaaaa gcggtggttt
    10441 tatctcaacg tctacacaaa gccattaccg aatctcaatc gaattactta agtactctgg
    10501 aaggaatttt attgttttat agaaataatg atatgcagca agactatcaa aacagccttt
    10561 caaattacat agtatttatc gatgaaattt ttgccttttc cccatcaaca agaagtgaat
    10621 taagcctcat taatttatta aattcattaa atgaggtgga attgatgaga aaacgtctgg
    10681 cgtttctgat gacagcacca gaatacgctt gttatgatag tcagtattgt tatgaagaca
    10741 aagtcagcgc attaaaaatc ctgtatcgtc atcatgacaa tgagctaatg gacaaatatt
    10801 tcacgactct tcttaaagag atcagtaatc aaccattaga agactgggat ataataataa
    10861 gtaatactgg tgaaaaattt agtgaagtgg gtctaaccaa atatgctcaa catctcgcat
    10921 ctttcgctga agaattttat ataaaagagc aaaaatcaca ttctgaaagt gaaatgagag
    10981 gactattttc atcacttgcc gaattatata gttttggtaa tgatactgcc agcgcaaacc
    11041 gagtgttcca tcagtatgtc cctgccttca atacagatca tatgattcaa tattttataa
    11101 atgccaaaga atggaacaat gccagggaat tattgataaa agaggaaatg ttgggtatgc
    11161 ataatctgat attgttggag aatatttgta tgcaaaaaaa tgcagaatgc atggcacaca
    11221 ttacctttac gcttaataaa ttaacaacac aaccagcaat aacaaaaata gatagcgtgg
    11281 gtaatgagca actttatcag attggcaaaa tctaccataa gttggaaatt aaacctgagc
    11341 cagagcaaca ggttttaatt cagtcgcttt atgaccgggc atcaggaagc gcctctgcaa
    11401 ctcaataaat gcatggtcaa aagggccacc acggtggccc tgacaatcag tagctatatc
    11461 cagaaagtta atcgcggaag tttttgaact ggaacggctg accgagatcg ccaccgcgca
    11521 ccatcgccat gacagcctgc aaatcatcac gagatttgcc agtcacgcgg atctcatcgc
    11581 cctgaatttg cgcctgcact ttcagtttgc tgtctttgat catcttgacg atttttttct
    11641 gagtggcgct ctcaatgccc tgtttcagtt tcgcttccac aaaccaggtt ttaccgctat
    11701 gaacgatatt ttccggtaca tccagtgaac tgccttcaat gccgcgcttc agcagcttgg
    11761 cacgcagaat atccagcaac tgattgacct ggaagtcgga ctcgctcaac actttgatgg
    11821 ttttgctggc gtcgttcagc tcaaatgagg cttcaacgtt acggaagtca aaacgggact
    11881 ccacttcgcg actcgcgtta tcgaccgcgt tacgtgcttc ctgaagatca acttcagaga
    11941 caatatcgaa agatggcatc ttttcctctc ccttcatttt tgatgcgaag cataatacct
    12001 gcaaagttaa ataaccaaca gtcctgcacc aggacgtatc ccttaattgt gcatagtcgc
    12061 aacagcatgc acaattaagg gatagcctta ttgaacaccc ggagtggttg tgggtgagga
    12121 ggaacaatga aaattaccgt attgggatgc ggtgccttag ggcaattatg gcttacagca
    12181 ctttgtaaac agggtcatga agttcagggc tggctgcgcg taccgcaacc ttattgtagt
    12241 gtgaatctgg ttgagacaga tggttcgata tttaacgaat cgctgaccgc caacgatccc
    12301 gattttctcg ccaccagcga tctgctcctg gtgacgctga aagcatggca ggtttccgat
    12361 gccgtcaaaa gcctcgcatc cacactgcct gtaactacgc caatactgtt aattcacaac
    12421 ggcatgggca ccatcgaaga attgcaaaac attcagcagc cattactgat gggcaccacc
    12481 acccatgccg cccgccgcga cggcaatgtc attattcatg tggcaaacgg aatcacgcat
    12541 atcggtccgg cacggcaaca ggatggcgat tacagttatc tggcggatat tttgcaaacc
    12601 gtgttgcctg acgttgcctg gcataacaat attcgcgccg agctgtggcg caagctggcc
    12661 gtcaactgcg tgattaatcc actgactgcc atctggaatt gcccgaacgg tgaattacgt
    12721 catcatccgc aagaaattat gcaaatatgc gaagaagtcg cggcagtgat cgaacgcgaa
    12781 gggcatcata cttcagcgga agatttgcgt gattacgtga tgcaggtgat tgatgccaca
    12841 gcggaaaata tctcgtcgat gttgcaggat atccgcgcgc tgcgccacac cgaaatcgac
    12901 tatatcaatg gttttctctt acgccgcgcc cgcacgcatg ggattgccgt accggaaaac
    12961 acccgcctgt ttgaaatggt aaaaagaaag gagagtgaat atgagcgcat cggcactggt
    13021 ttgcctcgcc cctggtagtg aagagactga agccgtcacc acgatcgacc tcttggttcg
    13081 cggcggtatc aaagtcacca ctgccagcgt cgacagcgat ggcaacctgg caattacctg
    13141 ctcgcgcggc gtgaagctgc tggcggatgc gccgctggtc gaagtggctg atggcgaata
    13201 tgacgtgatc gtgctgcctg gtggcattaa aggcacggag tgttttcgcg atagcactct
    13261 gctggttgaa accgttaaac agttccaccg ttccgggcgt atcgtcgcgg ctatttgcgc
    13321 cgcgccagcc accgtgctgg tgccgcacga tatctttccg attggcaata tgaccggctt
    13381 cccgacgctg aaagacaaaa ttcccgctga acaatggcag gacaagcgcg tcgtctggga
    13441 tgcacaggta aaattgctga ccagccaggg gccgggtaca gctatcgact ttggtctgaa
    13501 aattatcgac ctgctggttg ggcgcgagaa agcccatgaa gtggcatcac aactggtgat
    13561 ggcggcaggg atttataatt attacgagta gtgtcggatg cggcaaacgt cgcatctgac
    13621 cagatgcgac gtaaaaacta caaattacgg gcgatatacc ttcacattgt taaagccttg
    13681 ctcgcgcaga tagagcgcct gcagacggct catcaccccg cgctcacacc acagcagcca
    13741 ggttttgttc tggtcgagat cgccaaattt ggtgctcagt ttatagaacg gcagagaaac
    13801 cacatcaatc ccttcgactt tcagtggctt atcttcctgt tcatcgacag aacggatatc
    13861 gagaatcacg tcgttcgggc cgaagccgtt gacggtttcc acttccacca cttcctgctc
    13921 ggtctgctgg gcgatttcgc ggatatcaac gttattcgct tcctcaacca ctttatcgag
    13981 aatactgaag tcgaacttct cttcttccgc ttcaatcttc gatttaaccg ctttcaccgt
    14041 cgggcttttg gagatcacgc cacaatattc cggcatcgtg cgagcaaagt cttcggtgcc
    14101 aatctggcga gccaggttga tgatgtgctc tttgtcgtaa gaaatcagcg gacgcaggat
    14161 cagcgtgtca gagacgttat caatcaggcg caggttggtc agcgtctgac tggacacctg
    14221 gccgagcgct tcgccggtga ccagcgcctg tacgccgtaa cgttcagcca ctttagatgc
    14281 ggcacgcacc atcatacgtt tgaggataac gcccatctga ccgtcgtcga ttttctcgag
    14341 aatttccccg acaaccggtt cgaaattaat ggcgacaaaa cgcacgcggt gggagctgcc
    14401 aaagcggttc cacagataat gcgccacctg acgcacgcca atttcatgcg ctgcgccgcc
    14461 gaggttaaag aagcagtaat gcacgcggca gccgcgacgc atcaacatat aactggaaac
    14521 accggagtcg aaaccaccgg aaatgagcga cagcacatct tcctgggtgc caatcgggaa
    14581 accaccaata ccttcgtagc gacctttaat cagcaggagg cgatcgtctt ccacttccag
    14641 atgaacagtt acatccgggt tagtcagctt cacgcgcgcg gattcaatat gctgatttaa
    14701 accgccgccg acgtaacgtt ccacatcaat cgagctaaaa tcatgtttgc cacggcgctt
    14761 cacgcgtacg cagaaggttt tgccttccag ctgatcgcga tactgaacca acgctttctc
    14821 aaaaatatcg tgcatgtcgg taaacggcac gtcttcgact tcgagaatat ggtggatacc
    14881 cggaatacgg gtcagggcgt cgcgaatagt cagacgctgg ttttcatctt tcgcgcgaac
    14941 ttcgatgtta tcccagtggc ggacgacagc gagcgtctca tcatagtgct ttaaaacgtt
    15001 acgaatgttc ccggtaagga tttttataaa gcgcaagcgc acagattggc ttttgatggt
    15061 gatttccggg aacaatttaa tgataaactt catggcggca atggttcgtt ggcaagcctt
    15121 aagcgacttg tatagggaaa aatacagcag cccacacctg cggctgcatc caggcgcgga
    15181 agtataccac taacatcgct ttgctgcgca catcacctta ccattgcgcg ttatttgcta
    15241 tttgccctga gtccgttacc atgacggggc gaaaaatatt gagagtcaga cattcattat
    15301 gccgaagaaa aatgaggcgc ccgccagctt tgaaaaggcg ctgagcgagc tggaacagat
    15361 tgtaacccgt ctggaaagtg gcgacctgcc gctggaagag gcgctgaacg agttcgaacg
    15421 cggcgtgcag ctggcacgtc aggggcaggc caaattacaa caagccgaac agcgcgtaca
    15481 aattctgctg tctgacaatg aagacgcctc tctaacccct tttacaccgg acaatgagta
    15541 atggactttc cgcagcaatt cgaagcctgc gttaagcagg ccaaccaggc gctgagccgt
    15601 tttatcgccc cactgccctt tcagaacact cccgtggtcg aaaccatgca gtatggcgca
    15661 ttattaggtg gtaagcgcct gcgacctttc ctggtttatg ccaccggtca tatgtttggc
    15721 gttagcacaa acacgctgga cgcacccgct gctgccgtag agtgtatcca cgcttactca
    15781 ttaattcatg atgatttacc ggcgatggat gatgacgatc tgcgtcgcgg tttgccgacc
    15841 tgccatgtga agtttggcga agcaaacgcg attctcgctg gcgacgcttt acaaacgctg
    15901 gcgttctcga ttctaagcga tgccaatatg ccggaagtgt cggatcgcga cagaatttcg
    15961 atgatttctg aactggcgag cgccagcggt attgccggaa tgtgcggtgg tcaggcatta
    16021 gatttagacg cggaaggcaa acacgtacct ctggacgcgc ttgagcgtat tcatcgtcat
    16081 aaaaccggcg cattgattcg cgccgccgtt cgccttggtg cattaagcgc cggagataaa
    16141 gggcgtcgtg ctctgccagt actcgacaag tacgcagaaa gcatcggcct tgccttccag
    16201 gttcaggatg acatcctgga tgtggtagga gatactgcaa cgttgggaaa acgccagggt
    16261 gccgaccagc aacttggtaa aagtacctac cctgcacttc tgggtcttga gcaagcccgg
    16321 aagaaagccc gggatctgat cgacgatgcc cgtcagtcgc tgaaacaact ggctgaacag
    16381 tcactcgata cctcggcact ggaagcgcta gcggactaca tcatccagcg taataaataa
    16441 acaataagta ttaataggcc cctgatgagt tttgatattg ccaaataccc gaccctggca
    16501 ctggtcgact ccacccagga gttacgactg ttgccgaaag agagtttacc gaaactctgc
    16561 gacgaactgc gccgctattt actcgacagc gtgagccgtt ccagcgggca cttcgcctcc
    16621 gggctgggca cggtcgaact gaccgtggcg ctgcactatg tctacaacac cccatttgac
    16681 caattgattt gggatgtggg acatcaggct tatccgcata aaattttgac cggacgccgc
    16741 gacaaaatcg gcaccatccg tcagaaaggc ggtctgcacc cgttcccgtg gcgcggcgaa
    16801 agcgaatatg acgtattaag cgtcgggcat tcatcaacct ccatcagtgc cggaattggt
    16861 attgcggttg ctgccgagaa agaaggcaaa aatcgccgca ccatctgtgt gattggcgac
    16921 ggcgcgatta ccgctggcat ggcgtttgaa gcgatgaatc acgcgggcga tattcgtcct
    16981 gatatgctgg tggtcctcaa cgacaatgaa atgtcgattt ccgaaaatgt tggcgcgctt
    17041 aacaaccatc tggctcagct gctttccggt aagctttact cttcactgcg cgaaggcggg
    17101 aaaaaagttt tctctggcgt gccgcccatt aaagagctgc tcaaacgtac cgaagaacat
    17161 attaaaggca tggtggtgcc tggcacgttg tttgaagagc tgggatttaa ctacatcggc
    17221 ccggtggacg gtcacgatgt gctggggctt atcaccacgc taaagaacat gcgcgacctg
    17281 aaaggcccgc agttcctgca tatcatgacc aaaaaaggtc gtggttatga accggcagaa
    17341 aaagacccga tcactttcca cgccgtgcct aaatttgatc cctccagcgg ttgtttgccg
    17401 aaaagtagcg gcggtttgcc gagctattca aaaatctttg gcgactggtt gtgcgaaacg
    17461 gcagcgaaag acaacaagct gatggcgatt actccggcga tgcgtgaagg ttccggcatg
    17521 gtcgagtttt cacgtaaatt cccggatcgt tacttcgacg tggctattgc cgagcaacac
    17581 gcagtgacct ttgccgccgg tctggcgatt ggtggataca aacccattgt cgcgatctac
    17641 tccaccttcc tgcaacgcgc ctatgatcag gtgctgcatg acgtggcaat tcaaaagcta
    17701 ccggtcctgt tcgccattga ccgcgcgggc attgttggtg ctgacggtca aactcaccag
    17761 ggcgcttttg atctctctta cctgcgctgc ataccagaaa tggtcattat gaccccgagc
    17821 gatgaaaacg aatgtcgcca gatgctctat accggctatc actataacga tggcccgtcc
    17881 gcggtgcgct acccgcgcgg caacgcggtt ggcgtggaac tgacgccgct ggaaaaacta
    17941 ccaattggca aaggcattgt gaagcgtcgt ggcgagaaac tggtgatcct taactttggt
    18001 acgctgatgc cagaagcggc caaagtcgcc gaatcgctga acgctacgct ggtcgatatg
    18061 cgttttgtga aaccacttga tgaagcgtta attctggaaa tggccgccag ccatgaagcg
    18121 ctggtcaccg tagaagaaaa cgccattatg ggcggcgcag gtagcggagt gaacgaagtg
    18181 ctgatggccc atcgtaaacc agtacccgtg ctgaacattg gcctgccgga cttctttatt
    18241 ccgcaaggaa ctcaggaaga aatgcgcgcc gaactcggcc tcgatgccgc cggtatggaa
    18301 gccaaaatca aggcctggct ggcataatcc ctactccact cctgctatgc ttaagaaatt
    18361 attcatggca ggagtggcag catgcaatac aaccccttag gaaaaaccga ccttcgcgtt
    18421 tcccgacttt gcctcggctg tatgaccttt ggcgagccag atcgcggtaa tcacgcatgg
    18481 acactgccgg aagagagcag ccgtcccata attaaacgcg cgctggaagg tggcataaat
    18541 ttctttgata ccgccaatag ctattccgat ggtagcagcg aagagatcgt tggtcgcgca
    18601 ctgcgggatt tcgcccgccg tgaagacgta gtcgttgcca ccaaagtgtt ccatcgcgtt
    18661 ggtgatttac cggaaggatt atcccgtgca caaattttgc gctctatcaa cgacagcctg
    18721 cgacgtctcg gcatggatta tgtcgatatc ctgcaaattc atcgcgggga ttacaacacg
    18781 ccgatcgaag agacgctgga agccctgaac gacgtggtaa aagccgggaa agcgcgttat
    18841 atcggcgcgt catccatgca cgcttcgcag tttgctcagg cgctggaact acaaaaacag
    18901 cactgctggg cgcagtttgt cagtatgcag gatcactata atctgattta tcgcgaagaa
    18961 gagcgcgaga tgctgcctct gtgttatcag gagggcgtgg cggtgattcc gtggagcccg
    19021 atggcgcggg ggcgactgac gcgtccatgg ggagaaacta ccgcacgact ggtgtctgat
    19081 gatgtgggga aaaatctcta taaagaaagc gatgaaaatg acgcgcagat cgcagagcgg
    19141 ttaacgggcg tcagtgaaga actcggtgca acacgagcac aagttgcgct ggcctggttg
    19201 ttaagtaaac cgggcattgc cgcaccgatt atcggaactt cgcgggaaga acagcttgat
    19261 gagctgctga acgcggtgga tatcactttg aagccagaac agattgccga gctggaaacg
    19321 ccgtataaac cgcatgcggt cgtaggattt aaataagagt ccaggcctga tgagacgtga
    19381 caagcgtcac atcaggcatc ggtgcacaac tacgacagaa tacccagcgg ccagtgatga
    19441 ccgataaagt acaggatgcc tgcggaaatc accccggcga caatatcgtc gatcatgatc
    19501 cccatgccgc catgtacatt gcgatcaaac cagcggatcg gccacggctt ccacatatcc
    19561 agaatacgga aaatcacaaa cccggcggca acccactgcc agtcattggt cggcagcgcc
    19621 atgagcgtga tccacatacc aataaattcg tcccagacaa tgctgccgtg atcgtgcacg
    19681 cccatatctt tcgccgtttg atgacaaaga tagacgccga tacagatccc caacatcacc
    19741 accagcgagt agagctgcca gggtaaaaag gtcatcagat accagaaagg gatcgccgcc
    19801 agcgatccca tcgtgccagg aacgattggg cttaatccac ttccgaatcc gacagcaagc
    19861 agatgccacg gattactcat cttcaggcga cttttcgcga catctttatg gcgtgacaaa
    19921 atggtcatat cctttccagt caaacgtgac aggttcgccg tcacgaataa aacaaagccc
    19981 ttcgatatcg gcggtcattt gcccgataca ggtaaacggt acgccaaggt gtccgagagc
    20041 aacatccagc gcgccacggt tcagttccgg caccgtgaaa cacaactcgt aatcttcacc
    20101 gccagagagc gcccagcgca gcgcctgttc cggttcaaca tggcgagaaa gcgcatcaga
    20161 aaacggcagc aatgccaggt caatacgtgc gccgcagtcg ctggctttca cgatatgccc
    20221 gagatcggaa atcagaccgt cagagagatc gatagctgaa tttgccagat cgcgcagtgc
    20281 ctgcccctgt aaaatacgcg gcgatggacg gagatgacgt ttgatcaagt agtcagcatc
    20341 tttagcatcg gcaacctgca aacggttttg caaaatcgcc agcccggcgg cgctatcgcc
    20401 cggtgtaccg gtcacataga tccagtcacc cggtttcgcc ccagagcgcg ttaaggctcg
    20461 tcccatcgga acaaagccgt ggatacccaa cgtcattgat aatggcccac gcgtggtatc
    20521 gccgccaatg agttgcatat cgtaataatt gagaagatca aacaaactgt cgctgaagga
    20581 ctcaagccac gcttcgtcta cgtccggtaa ggttaatgcc agcgtcagcc aggccggatc
    20641 ggcccccatc gctgccagat cgcttaggtt caccgccagt gctttataag ccagatcagc
    20701 aggatcgata tcagggagga aatggttgcc cgccaccagc gtatcagtgc tgatcgccag
    20761 ggtctgtttc tcggggatat tgagaagagc gcagtcgtcg ccgatgccca gttcgacatc
    20821 aagacgagaa cttcttacac ggtcaaaata acgggcaatc agggagaact cgccacatgc
    20881 catacgttat gcctcagcag gtaaaaaaga aaaggccggc gatcatggaa cagtcttccg
    20941 tgaatctacc ggcctggata ttactttttg ttagggcgaa tcacaggtgc tgctttatcg
    21001 agtacgccgt tgacgaactt atggctgtct tctgcgccga acgatttcgc cagttcgatc
    21061 gcttcgttaa tggccacttt gtatggcaca tcactacgtt tagacagttc gtacagcgca
    21121 atgcgcagta ctgctttttc tacctggccc agttcttcca gcaggcggga caggtagggc
    21181 ttcatcagtc cgtcgaggta tgcggtgtta gtcgccaccc cggccagcag ctcacggaag
    21241 tacaggacgt caacgtcttt tacatcctgt tcagccagga actggtattc aacatcagcg
    21301 atgtcgttct gggacaactg ccaggagtag agcgcctgga cggcacactc acgagcgcgg
    21361 cgacgagcag caggtttcac ggatttcccc ttactaattt caggccttga tggctttcaa
    21421 tacattaatc atttcaagcg cggtcagtgc agcttctgca cctttgttgc cagctttggt
    21481 gccagcacgt tcgatcgctt gttcaatgct ttcagtggtc agaaccccaa aagcaaccgg
    21541 aatttcgctg tcctgggcaa catgcgccag gccgttgctt gcaccaccag cgacatattc
    21601 aaagtgggca gtgccaccac gaataaccgt acccagcgca atcaccgcgt cgtatttacc
    21661 ggttttagcc agtgcacccg ccgccagcgg cagctcatag gcaccaggca cccaaacaac
    21721 ggtaatgttt tcatctttta cctgaccgat acgtttcagt gcgtcaattg caccttccag
    21781 caggctgtca ttgataaagt tgttgaaacg cgcgatggtg atggcgacgc gagcgtccgg
    21841 ggtagcaacg ttagcttcaa taatgttcat tttcttcctt cgggttcgag tatggccccg
    21901 caggggggcg gattttagca taatatttcg tgcgctgctt ccctttcgag ccgggagagc
    21961 atgcccccac taaatgcagg caaacatccg ggcctacatg acgtatctct ttgaatttaa
    22021 attggggggc gtcggctaat ttctcaagcc ctggcagcga gcataatcca cgggcgtcgc
    22081 tgcctaatag tttaggtgcg atatagacaa tcagttcatc gactaaaccc gcctgtagca
    22141 atacgccagc gagcgttggc cccgcttcca cccagatgct gttaatctgc tgtttaccca
    22201 gttgcatcat cagtacaacc agatccagat gacctttatg ctctggaatc agcaaggtac
    22261 gcaccgtttc cggccactca cgagaatctt cctgcgtacg cgcgaaccag gtttcgccgg
    22321 gctgctgcac aatgcgatgt tccggcgtca cgcgattttg gctatcaatc acaatacgta
    22381 ctggctggcg gagattttgt tgcgggtaga gcgcctgagt ttgttcatcc agttcagacc
    22441 aacgcactgt taaggctgga tcatccgcca gcaccgtggc gctgctggtt aaaatggcat
    22501 gactttgtgc gcgctgtcgt tgtacgtcgc gccgcgcctg aggcgaagtg atccactggc
    22561 tttcgccgct cgccatcgcc gtgcggccat caagcgatgc gccaagtttt aactgaatat
    22621 aaggaaagcc ggtgcgcatc cgcttgagaa agcctttatt caattgctcg gcttcactca
    22681 tcatcagccc gtggctgacg tcaatgccag cctgttgcag acggtaaagt ccacgcccag
    22741 cgacctgcgg gttaggatct tgcatcgctg cgaccacgcg cgctacgcca gcggcgatga
    22801 gtgcgtcaca gcacggtggc gtacggccat gatggctaca gggttcgagt gtcacatacg
    22861 ccgttgcgcc tttggctttt tcacccgcca tacgcaacgc gtgtacttcg gcatgtggtt
    22921 cacccgctcg ttggtggtaa ccttcaccga caatttcgcc atctttgaca atgacgcacc
    22981 cgacattcgg gttggggtgg gtggtaaaac gtcctcgttg cgccagcttt agcgcccgcg
    23041 ccatgtaata ctcgtcctgc acggcttagt cctccaggcg cgcgatctct tcgccaaatt
    23101 ctttgatatc ttcgaaactg cggtagacag aggcaaaacg gatataggcg actttatcga
    23161 gctttttcaa ttgctccatc accagattgc caatcatctt gctcggtact tcgcgctcac
    23221 cggtggcgcg cagctgcgat ttaatatgat tgattgccat ttcaacgtaa tcggaactca
    23281 ccggacgttt ttccagcgcc cgcagcattc cgctacgcaa tttctcttca ttaaacggtt
    23341 cacgcacgtc gttgcttttt acaacacgcg gcataaccag ctccgccact tcaaaggtgg
    23401 tgaaacgttc attacacacc agacactgcc gacggcggcg tacggatgaa ccctcgccca
    23461 cgagacgaga gtcaattacc ttagtgtcca cggcgaaaca gaatgggcaa tgcatacggc
    23521 gtcctgacca ggtggttaac ggaaatctat tttaccctga actgacgata caacaaaggc
    23581 gcaacgcttt tgagataaag gatcgatgct ttcgctgctt ttaccctcta ccattaggct
    23641 aattgcgacg agaaagggac gacctatgaa tactaacgtt tttcgactgc tcctgctggg
    23701 aagcctgttc agccttagcg cctgtgtgca gcaaagtgaa gtgcgacaga tgaaacacag
    23761 cgtcagcacg ctgaaccagg agatgacgca actcaatcaa gaaacagtca aaatcactca
    23821 gcaaaacagg ctgaatgcaa aatccagcag tggggtttac cttctgcctg gagcgaaaac
    23881 accggcaaga ctggaaagcc agatcggtac tttacgtatg tcgctggtga atattacgcc
    23941 tgatacagat ggcaccaccc ttacactacg tattcagggt gagtcgaatg atcccttgcc
    24001 cgcattcagc ggaactgttg aatatgggca aattcaggga acaatagaca actttcagga
    24061 aatcaatgtg cagaatcaat tgattaatgc ccctgccagc gtcctcgccc ccagcgatgt
    24121 tgatattccg ttacaattaa aggggatttc tgtggatcag ttaggctttg tacgtatcca
    24181 cgacattcaa cctgttatgc agtaaacgta tttcgggacg attttgtgcc tcccgcaaca
    24241 tctttccccg tcattttgtt actctgctta catcacttgg attgatagta aaagtttgca
    24301 acaagggcga aagtcagtac aatccccgcc cgaatgtgtg taaacgtgaa cgcaatcgat
    24361 tacgtaaatg atagaactgt gaaacgaaac atatttttgt gagcaatgat ttttataata
    24421 ggctcctctg tatacgaaat atttagaaac gcaatttgcg cctttttcac tcccgcaagg
    24481 gattttcaaa cagtggcata catatgaaaa aaacattact ggcagccggt gcggtactgg
    24541 cgctctcttc gtcttttact gtcaacgcag ctgaaaacga caaaccgcag tatctttccg
    24601 actggtggca ccagagcgtt aacgttgtcg gaagctatca cacccgtttc ggaccgcaga
    24661 tccgcaacga tacctacctt gagtacgaag cattcgctaa aaaagactgg ttcgacttct
    24721 atggttatgc ggatgcgccg gtattcttcg gcggtaactc cgatgctaaa ggtatctgga
    24781 accacggttc tccgctgttt atggaaatcg aaccacgttt ctccatcgac aagctgacca
    24841 atactgacct tagcttcggt ccgttcaaag agtggtactt cgcgaacaac tacatttacg
    24901 acatgggtcg taataaagat ggtcgccaga gcacctggta catgggtctg ggtaccgata
    24961 tcgacactgg cctgccgatg agcctgtcca tgaacgtcta tgcgaaatac cagtggcaga
    25021 actatggcgc agcgaacgaa aacgagtggg acggttaccg tttcaaaatt aaatactttg
    25081 tgccgattac cgatctgtgg ggcggtcagc tgagctacat cggcttcacc aacttcgact
    25141 ggggttccga tttaggggat gacagcggta acgcaatcaa cggtattaag atccgtacta
    25201 ataactctat cgcttccagc catattctgg ctctgaacta cgatcactgg cactactctg
    25261 tcgtagctcg ttactggcac gacggtggtc agtggaacga cgatgcagaa ctgaacttcg
    25321 gcaacggcaa cttcaacgtt cgctctaccg gctggggtgg ttacctggta gtaggttaca
    25381 acttctgatt atgaaaatgc cgggatttat tcccggcatt tctgattgtt aacaacgtgt
    25441 gctttgttca tgccggatgc ggcgtaaacg ctttgtccgg cccacaaaag cacgtaaatt
    25501 caatatattg ctgaaataac gtaggcctga taagcgtagc gcatcaggca attttacgtt
    25561 ttaatcactt cttcttgttc atcatcgctt tcaggtcagc gaatgggttg tacttcgctt
    25621 caccgacatc tttctgcgca tcttcccctg cgataacggt cgtaccatac tgatccgctt
    25681 cggtatattt cgaatgctca tgatcgtggc aatagagaca caacaattcc cagttactgc
    25741 catcttccgg gttattggta tggtcgtgat caatgtggtg aacggtaagt tcacgcaggt
    25801 tagaataaac aaactcgcgg gaacagcgac cgcagaccca cggatagatt tttaatgctt
    25861 tttcgcgata gccgctttct aaccgtgcat agttttttgg gatgatagcc atgaaaagtt
    25921 acctggatga aatgtagggt tacgcgcagt ttcccaagat gcggggattt tcgcaatctc
    25981 catacatcaa aaaaaatccc gatcttctga ccgggatttt caacatcaac gggaacttga
    26041 ttaaggcaga attgacggct gatccgcccc ttctttttcc actttctgct gcaacatgtg
    26101 ttcgcgcttc atacccagtt tcagagccaa cgcagatgct acgtagatgg aagatgcagt
    26161 gccgatggaa acaccgataa gcatggtcag cgagaagcct tccagtaccg gaccaccgaa
    26221 gagatacagc atcaggataa ccatcaaggt agtaccggat gtgatcaagg tacggtgcag
    26281 cgtctgggtc aaggacacgt taaagatttc gtaaggcgta ccgcgacgga tcttgcggaa
    26341 gttttcacga attcggtccg ataccacgat actgtcgtta agcgagtaac cgataaccga
    26401 catcaacgat gccacaatgg tcaggtcaat ctcgatatgg aataacgaca aaatacccag
    26461 cgtaatgata acgtcgtgcg ccagcgcaat aaccacccct gccgccagtc gccactcaaa
    26521 gcggaaacct acgtacacga ggatagacag cagcgctgcc atcaacgcca tcgcaccggt
    26581 ttgcgcaagg tctgccccca cgctcggacc gacgaactca atacgcttca ctgctgcatt
    26641 ctgattggtg gattcgttaa tcaccttcag aacttggctg cccagcacct gaccgccggt
    26701 ttcgccttca gcaggcggca tacggaccat gatgtcgtgg ctgctaccaa agttttgcag
    26761 cattggctct tcaaaaccgg ctttttgcaa tgcatcacgc attacgtcaa tttcagccgg
    26821 tttttcgagc gtaatttcga taaccgtacc accggtgaaa tccagccccc agttaaagcc
    26881 gcgcacgccc ataataacga tagcagcgat taacagcaga ccagagatgc cgaaagccca
    26941 gtagtcccag cgcataaagt catagacttt acggccgtgg tttagttgtt caacagtata
    27001 ttcctgtgcc acatcgcact cctcagattg acagcttctt gacgcgcttg ccgccatata
    27061 gcaggtttac gatggcacgc gtaccgacaa tcgcggtaaa catcgacgtc gccacaccga
    27121 taccggtagt aatcgcgaac cctttaattg ccccggtacc cactgcgtac aggatgatga
    27181 ctttaatcag cgtggtgatg ttcgcatcga agatagaact gaatgcgcca cgataacctt
    27241 catcaattgc ctgttgaaca gtacgtccgt tgctcaactc ttctttaata cgttcgttga
    27301 tcagtacgtt cgcatcgacc gctaccgcaa gggttaagac gatacccgcg atacctggca
    27361 tactcagtgt tgcgcctggc aacagcgaca taataccgac gatcaagatc aagttggcaa
    27421 tcagagcact ggtcgcaatc agaccaaact tcttatagaa gatgatcatg aacagaatag
    27481 aaaccagcag accggcaagg caagcctcca gcccctgctc aatgttctgc atacccaggg
    27541 tcgggccaat tgtacgttct tcaacaatct gaatcggcgc gatcaacgca ccggcacgca
    27601 gcagcagtga cagctgacgg gcttcgttcg ggttgttgat gccggtgata cggaagctgt
    27661 tacccagacg agactggatg ttggcgatgt taatcacctc ttcctgtttc accagaaccg
    27721 cacgaccatt tgcatctttc ttaccgctgt ctttgtactc cacaaacagg gttgccatcg
    27781 gtttgccgat gttgtccttg gtgaagttag acataatgtt gccaccagcg ctatcgagcg
    27841 agatgttaac ctgcggctgg ttgtattcgt cctggctgga agtggagtcg gtgatatggt
    27901 caccagtcag aattacgcgt ttgtacagca caaccggctg accttcgcgg gtctgtttca
    27961 cttcagagtc gcccggtacg cgaccggatg ccgcagcggc ctgatcaaca ttggtgttta
    28021 ccagacggaa ttccagcgtt gccgtcgcgc ccagaatctc tttcgcacgc gcagtgtcct
    28081 gaataccagg cagttcaaca acgatacggt cagcaccctg acgctgaacc accggctccg
    28141 ccacgccaag ttggtttaca cggttacgca ggatattaat gttctgctgc accgcatatt
    28201 cacgcgcttc actcagacga gcatcgctca ttaccgcacg cagctggttg ctgccctggc
    28261 tgctaatcac caggtccgga tggcgcttgc tcagatacgc aatggcttca tcacgagctt
    28321 tagcatcgcg gaaagtgatg ctcaggccgt agttgttttc tttacgaaca gtggtatacg
    28381 ggatgccctt ttcgcgcagg tcactgcgta ggctatcgat attttgttcc tggagtttgc
    28441 caagcgcggt gtccatatcc acttccatca ggaagtgaac gccgccacgc aggtcaaggc
    28501 cgagcttcat cggctcagca tgaatagctg ccagccagcg cggcgttgcc ggggcaaggt
    28561 taagcgccac gacgtatttg tcacccataa cgcccattaa tgcttcacga gcgcgcaact
    28621 gggtgtcagt ggagtcgaag cgcgcaagaa tagcgccctc ttccagtgcc acagacttag
    28681 cagttatttt ttcttcttgt aacgttttct ggacctggat cagcgtttgc tcactggcgg
    28741 cgactccgcg cgcaccagtg atctgaacag ccggatcctc accaaacagg ttgggaagcg
    28801 catacagcag accgatgaca atcaccacga tcagcatgac gtacttccac aaaggataac
    28861 ggtttaacac ggcaattccc ttagggaaaa attttaatta cagcgccttc atggtgcctt
    28921 tcggcaggac ggcagctacg aagtcacgtt taataactac ttcagtggtg tcattcagcg
    28981 cgatagcaat gtagccgttt tccgctactt tggttacgcg accaaccagg ccaccgttcg
    29041 tcagaacttc atcacctttg gcaatggagt ccatcagctt tttgtgttct ttggtgcgct
    29101 tctgctgtgg acgcaggatc atgaaataga aaatcagacc gaataccacc agcatcaaaa
    29161 tcaaagacat cgggctacct tgcgccggtg cacccgttgc cgctaccgca tcagaaataa
    29221 aaaagctcat taaatttccc tcattattaa tattaatcaa cgttcaaagg tggtacttct
    29281 cgcccctgac gctggtaaaa atcagttacg aagctctcta atttaccctc ttcaatagcc
    29341 ttgcgtaaac ccgccatcaa acgctggtag taacgaaggt tatgaatggt gttgagtcgc
    29401 gcgcctaata tttcgttgca acggtcgaga tgatgcaagt aagcgcgtga ataattgcga
    29461 caggtgtagc aatcacactc aggatcgagt gggccagtat cgctcttata tttcgcattg
    29521 cggattttca ccacgccatc ggtcacgaac aaatgaccat ttcgggcgtt gcgggttggc
    29581 attacgcagt caaacatatc gataccgcga cgtacgcctt caaccaggtc ttccggttta
    29641 ccaacgccca tcaggtaacg cggtttgtct gccggaattt gcggacacac atgctccaga
    29701 atacggtgca tatctgcttt cggctcaccc acagccagac cgccgacagc gtagccatca
    29761 aagccgatat ctaccagacc tttaacagat atatcacgta aatcttcgta aatactgccc
    29821 tgaattatac caaataacgc atttttgtta ccaagactgt caaaacgctc acggctacgc
    29881 ttcgcccaac gcagagacat ctccatagag cgttttgcgt aatcccagtc agcaggatac
    29941 ggcgtacact catcaaagat catgacgata tccgaaccaa gatcgtactg aatctccatc
    30001 gacttttccg ggtcgaggaa aatcgggtcg ccgttgatcg ggttacggaa gtgaacgccc
    30061 tgttcggtga ttttacgaat atcaccaagg ctgaagacct ggaagccgcc ggagtcggta
    30121 agaatcggtc ctttccactg cataaaatcg tgcagatcgc cgtgcagttt catgatttcc
    30181 tggcccgggc gcagccacag gtggaaagtg ttgccgagga taatttgcgc gccagtggct
    30241 tcaacttctt ccggcgtcat cccttttacg gtgccgtagg tgccaacagg cataaaacaa
    30301 ggcgtttcca ctacgccacg atcaaagacc aggcggccac ggcgtgcgcg accgtcggtg
    30361 gtgtccagtt caaatttcat tttttctcca ctacgtcaga aaaacagtcc aacgtttaaa
    30421 ccagcgccgc ggaattactc cccgacgcgc tcattaattg cctgcggatt gtacgtgata
    30481 aacatcgcat caccgtaact aaaaaagcga tatttctctt ctaccgccgc tttataggca
    30541 ttcatggtgt gttgataacc ggcaaaggcc gaaaccagca taatcagcgt cgactctggc
    30601 aagtggaagt tcgtcaccag cgcatcgacc actttgtact ggaagcccgg atagataaag
    30661 atttgggtat cgtcaaagaa cggttcgata agatcgtttt ttgccgcctg tgccgcgctt
    30721 tccagcgaac gtactgaagt ggtgccaacc gcaatcaccc ggttaccgcg cgctttcgcc
    30781 gccagtaccg cgtctaccac atcctgcggt acttcagcgt attccgagtg catgatgtga
    30841 tcttcaatgg tgtcgacgcg caccggctgg aaggtgcccg caccaacgtg caacgtcaca
    30901 aacgccatct ccacgccttt ggcgcgtaat ttttccagca aaggctcgtc aaaatgcaga
    30961 ccagcggtcg gggctgcaac cgcgcccggt ttttcgctat aaacggtttg ataaagttcg
    31021 cggtcagcgt cttcgtccgg acgatcgata tacggcggca gcggcatatg gccgatgctg
    31081 ttgaggatat ccagtaccga gcgttcatca ttaaattcga cttcaaacag tgcgccgtgg
    31141 cgcgcggtca ttgttgcgtt gatactttcg tcatcgccca gcaacagttc tgcgccaggt
    31201 tttggcgctt tcgaggcgcg aatatgcgca agaatgcgtt tgtcgtcgag catccgctca
    31261 accagcactt caatcttgcc gccgctggct ttacgcccaa acagacgcgc cgggatcacg
    31321 cgggtattat taaaaaccag aagatcgccg gggttgagct tatcgagtaa atcggtgaaa
    31381 gtaccgtgcg tcagcgcacc cgtcggcccg tccagcgaca gtaaacgaca gctactgcgt
    31441 tcaggcatgg gatagtgggc aatcagggat tcgggcaatt caaaggagaa atcggtaacg
    31501 cgcatgacac tgactcagac taaaataaga gtcgggtagt ctagtgccgg ggcgctctcc
    31561 ctgcaacctt tacccccctt tggatagaat ataatcgatg aattttttag ctcacctgca
    31621 tttagcccat ctcgcggaaa gctcgctttc tggcaattta ctggctgatt tcgtacgcgg
    31681 aaatcctgaa gaaagttttc cgcccgacgt cgtggctggc attcatatgc atcgacgtat
    31741 tgactgacaa tctgccggaa gtccgcgaag cacgggagtg gtttcgtagt gaaacgcgcc
    31801 gcgttgcgcc tattacgctg gatgtcatgt gggatcactt tctttcccgc cactggtcgc
    31861 agctgtcgcc ggactttccg ctacaggaat ttgtctgtta tgcccgcgag caagtgatga
    31921 cgattttgcc ggactcaccg ccacgtttta tcaatctgaa caattacttg tggtcagagc
    31981 agtggctggt gcgctatcgc gatatggatt tcatccagaa cgtgttaaac ggcatggcaa
    32041 gccgccgccc acgtctggat gccctgcgtg actcctggta cgatttagac gctcattatg
    32101 acgccctcga accccgcttc tggcagtttt atccgcggat gatggcgcag gcgtcacaca
    32161 aggcgttata aaacgttagt tcatccatac cgtagccgaa atggcaggca aagccagaat
    32221 cccgtcagtc agttgcccat gcccttcttt gcattgccat tgcacggcat tgagaaacgg
    32281 tgacgcgggt agcaccactt cactggcctc gccacggttg attgccacca gtacacgttg
    32341 ctgattcagc acgcggacaa ataccactac gttatcttcc gcatacagca cctgacagcc
    32401 gccacgacgc agcgcctgac ttttcttacg cagcacaatc attcgccggt acagcgcgaa
    32461 taacgccgta tcctgctttt ccacctgcca ggggaacggt ttacggcaag acggatcgtt
    32521 tttaccatcc agtcccactt catcaccgta ataaatgcac ggtacaccag gccaggtgaa
    32581 cagccagacc accgccagcg gcaggcgcgc aatatcccga ccgagcagcg ttttaaatcg
    32641 cgcagtatcg tggctatcga gctgattaaa catacgtaat tgttgttgat gagaaagccc
    32701 tgcgcggtaa ttatccatcc aggccataca ggtttgggcg tcaatttgct gcggatcata
    32761 agagatgtcg gtattggcaa gaaatcccca caacgggaat gtgaagccac gatagttcat
    32821 ggcagcatct tccacatcgg cctgtaacca ttgccgtgca tcgccaaaat gttcgccgac
    32881 aatatacgct tccgactggg tttctttcgc cgcttcggtg atcccggcaa cgtgctgcat
    32941 attattgcgc gccccacccg cctcccccag catatgcacc acatccagcc gccagccgtc
    33001 catattccac ggcgctttca gccagtggcg gacaatactg tcttccccgc gataaatttc
    33061 attcaccaga ctttccgact gataatccag cttcggcaag ctggcatagc caagccagtc
    33121 gagcgccgtg ccatcatcac taaacaagta ccagtcgcgc cagggcgatt cagagttgtg
    33181 acaagctccc cccgtgccaa gattgtgcct gtcaaaccag gcatgggaat cgccactgtg
    33241 gttaaacacg ccgtccagca ccagccgcat tcccagctgc tgcgtattgt gtcgcaaacg
    33301 cagcaacgcc ccatcaccgc caaactgcgg atcgacatgg cgataatcct cggtatcgta
    33361 tttatgtacg ctgggagctt taaacaccgg attgagatac agcgctgtca cgccaagctt
    33421 tttcagatac ggcagttttt cgcttatccc gtccagatcg ccgccataga acgttgatcc
    33481 gcccgcctgc gccgtgaccg gttcatccca gtcacgcaag atgatctctt gtccggctgc
    33541 atgatggtaa tagacatgat cctgttcagc ttcacgagga agactacgcg caaaacgatc
    33601 agggaagatc tgataaaaaa tctgatccgc agcccattgt gggccgatat ccggtacatc
    33661 gacggcaaac tgctccagtc gtgccggcgg cattcggctg aagccctgcg gtgtaaacca
    33721 acgctggcga tcgtgccaca gcagtttgaa actgtaacgc cgccggggtt gtccgctgga
    33781 gagatcaatc gccgcacgcc atgcggtgac gcctggctgc ggctgactgc gctgcttatg
    33841 cattggtact gacatttctt cgttatcgtg ttctgtacgc agcataatgc gctgcggtgg
    33901 gtcttcgccc gtcagccaca gtgtaatgag cagttgatct ttgctttgtt taacaaatgg
    33961 gggcaccggc aggtgccatg catttaacat cataattccc ctgtgatgaa aatctgatca
    34021 ccttgccata gcctaatgca ggatcactca tcatctgaag gatatttggg ggaggaggac
    34081 gggggcggat aactttcgga atagccggat caggatttcc tcctgaaccg gcttacagaa
    34141 cgcaagtatt attggttttc agccagctga cgatcgtggc ggcgtttaaa catccagcca
    34201 atcaacagca caacaatcca cgcgaaaccg acatacagcg agatacgcgt atccgggtga
    34261 taaccaatca acccgataat aaagagcagg aaaatcagcc cgccgatggt cgttgctacc
    34321 ccacccggca ctttaaattt cagcgcctta acttcttctg gcggcaaacg gcgacggaag
    34381 gcgatttgcg acagcaggat cataatccac acccataccg tggcgaaggt tgccagcgaa
    34441 gcgatcacca ggaagacgtt ttccggcatg atgtagttca gatacaccgc aaacagcagc
    34501 gcggtagtca tcaccagcac cgtaacccac ggaataccgc gacgcgacgt tttgctgaaa
    34561 attttcggtg cgctgccctg ctctgccata ccgtggagca tacggcctac gccaaatacg
    34621 tcactgttaa ttgccgacag cgaagcggtc agcacaacaa agttaagaat gctggcggca
    34681 aaggtaatgc ccatatgctg gaacgtcagc acgaacgggc taccggcagt gccaacctga
    34741 ttccacgggt agatagacat aatgacgaac agcgtaccga cgtagaacac cagaatacgc
    34801 atcggcacgg agttaatcgc acgcggaatc gatttctcag gatctttcgc ttcaccggcg
    34861 gtaatcccga taatttcgat cccaccgtaa gcaaacatca ccatttgcaa cgacattacc
    34921 atgccaagcc agccgttact gaagaagccg ccgttgctcc acaggttatg aataccggtc
    34981 ggttgcccgc cgttgccaat cccccagatg atgatgccga aaccggcgac aatcatgatg
    35041 atgatggtgg cgactttaaa gaacgagaac cagaattcca gctcaccgaa gaccttcacg
    35101 ctcatcaggt ttacggcgca aatgatcagc accacgctca gtacccaaat ccagtgcggc
    35161 accgtcggga acaagacacc catatagata ccaaaagcgg tcacatcggc gatggcgaca
    35221 ataaggattt caaagcagta ggtccatccg gtaatgtaac ctgccagcgg gccaaggttt
    35281 tcctgcgcat aacgcgagaa agagctggcg gccgggttat gtaccgacat ttcccccagc
    35341 gcacgcataa tgatatacgc cgcgatacca ccgataatat aggccaacaa cacgctcgga
    35401 ccggctattt tgatggcgtc tgccgaaccg taaaacagcc cggtgccaat tgctgaaccc
    35461 agtgccataa agcgtatgtg gcgggtactt agcccacgct ttagcttatt cttactttcc
    35521 atcaattcct aacccatcaa cacaataaaa aaccacgggg catcgcagcc ccgtggttaa
    35581 aacaaatgtt cagtgattta gtgagcgctg gaggtcacct gacgacctgc cgcgcgatcc
    35641 cagataatgg ccagaaccac catcaccact gttggcatta accacgccag accttgttcg
    35701 gccagcggta aacgctgcgc ccaggacggt aagatatcgc tgaatgcaga tgctttgatc
    35761 ccgtcgagaa taccaaaaag caggctgata aacatcggcg gagcaatcac gcgggacgaa
    35821 ttatgccacc atgagcgtgt aaaacttaat acaaccagtg cgatacacgg cggataaata
    35881 gcggtcagca ccggtacgga gatctggatc agctggctta agccgaggtt agacaccacc
    35941 atcgagaagc cgccgaggat aaacaccagc gtacgataag agagcggtac gtactgggca
    36001 aagaattctg cacaagcaca ggtcaggcca accgccgtga ccagacaggc gatgaagatt
    36061 aacgccgcca gcaggaagct accgccgccg ccaaaggtgt gctgaacgta agcatgcaga
    36121 atagcagcgc cgtttgcaga ctgatcgacc agcgacgcgc tgtctgaacc cagacagaac
    36181 agcgccaggt acagcaaagt cagaccaaca cccgccatca ggccagccca gacggtataa
    36241 cgggtcagca gacgcgcttc ggtaacgcca cgagaacgcg ccgcgttaac aataacgata
    36301 ccaaacacca ttgcgcccag cgtatccatg gtcagatagc cgttaacgaa gccgttagaa
    36361 aatgcagcgt tttgataagc ctcagtcgcc gtgctgatag aacccgccgg ccagacaatc
    36421 gcgacaacag acaggatgac cagcgcgata attttcagcg gcgcaaggaa gttgcccacg
    36481 gtatccagca gcttgcccgg atagagcgaa accagaataa cgatagcgaa atagaccagg
    36541 ctgtagataa acagcggcag cgcggaatca cccgtcagcg gcgcaatccc cacttcaaag
    36601 gaaacggtag ctgtacgcgg cgtagcgaaa agcggcccca ccgccaggta acaaaccgtt
    36661 gccagcagta cgccagcgac tttaccgatt ggcgtgctga ggctgtcaac accgccgcca
    36721 acttttgcca gcgccactac cgttaatacc ggcaggccaa cggcagtaat gaggaagccg
    36781 aatgccgcag tccagacgtg ttcgcctgcc tgtaagccga ccattggagg gaaaataatg
    36841 ttacctgcgc cgacgaacaa cgcaaatgtc ataaagccca gagcgatgat atcgcgcgat
    36901 cttaattgat gggtcataaa tcatactgcc tgtggatgtg gtgttgtaac gttaaatttt
    36961 tgtgccaatc cctggggatg atacagcggt atatttaaac aattttagcg ggaaatactc
    37021 ccaaccaggc gcggcgaaga atagtttttc gatttaccgc gttaatgcag tcagtatgac
    37081 agcacctgaa gcgcaattta aacgcttata acgttttaaa gcaaggaggg atctaaaaac
    37141 cagaggtata tagcgataag aaatcaccaa ccagtataaa acactatttc cgtgaaaaag
    37201 acatggctaa tcatgcgaac aaaaaatcac caaccgttgt aaaatctaac gcccgtattt
    37261 tgactgcaaa gaaaataagc cagcatcggc tggcttatgg caataaaaga tgacaaaggc
    37321 ggattaatcg ctgtttttgg caattaaacg ttccgggata acaaaactga aacgtgttcc
    37381 ttttcctact gtactctcaa tattcaggcg actttcgtga tgattcaccg catgtttcac
    37441 gatcgctaac cctaatccgc taccgccggt ttgccgggaa cgcgctttat caacgcgata
    37501 aaaacgctcg gtcaggcgcg gaatatgctc cggtgcaatg cccggtccgt tatcttcaac
    37561 gctaaattcg gcaccgtgcg gcactcgctg ccagcgtacg gtgatatgcg tgccttccgg
    37621 cgtatgattc acggcgttat agaccaggtt cgaaatcgca ctgcgtagct gatcttcgtt
    37681 gccagacacc ttgaggccgt tatctatctc aaaggtaaat gtctgttttt tctgactcag
    37741 agtctgagcc tcgcgctcaa caacgcgcag catcatcggc acatcaacct tttcattgag
    37801 caattgcgtc ggcgaggctt caattttcga cagcgttagc aactgcttca ccagcccttc
    37861 catccgctgg gtctgctcgc gcatggtgtg caacgctttt tcgcgtaccg cgccttccag
    37921 cggctgctca tccatcatct ccaggtaacc ctgtaacacg gtcaatggcg tacgtaactc
    37981 atggctcacg ttggcaaaaa agttacgccg cgccccttcc agttgatgca tttgcgtgac
    38041 atcacgcgcc accatcaaca actgtttgtg ggtataaggc atgacgcgaa tttccagatg
    38101 ccgcccggtg ttgagcacca gattgagcgg gcgagaaaaa tcacgcgttt tcagatattg
    38161 cgtaaactcc gggtaacgca gtaggttaag gatgttctgc ccgttatctt ccggccagcg
    38221 caaaccaaga atttgttgcg ccagaccgtt acaccagaaa ataccgccct cttccgtggt
    38281 cagcaccacc gcgtcgggca gcgactccgc gccgctacga aagcgtttaa tcagattgcc
    38341 cagttcacgg cggcgttttt tatttcgcag ctgcatctgg tgtaagccgt atagtagcgg
    38401 ttcccagcta ccacgccccg gtggcggggt catactgcga tccacccaca gccaccatga
    38461 aaggcgcaat aaattccaga aatgccagat aagcagtcct gttaccgatg ccagcaaaaa
    38521 ccagggcagg taaccaaaaa atgcacccag gatgaaagcc gggaggcagc aaagtagcag
    38581 ctccagcacc agccttttcc acgacagccg ttccagcacg taagatactc cagttaagaa
    38641 atcataagcc ctgctctgcg tccgatgagc aaggcgttaa aagcgggttg aaaaacgata
    38701 tcctgtaccg cgcacggtct gcaccatgcg gtcatgcccg ccgggctcca gtgctttacg
    38761 caggcgacga atgtggacat cgaccgtgcg gtcttccaca tacacgttag ttccccagac
    38821 gtggtttaac agctgctcgc ggctgtacac gcgctcagga tgcgtcataa aaaagtgcag
    38881 cagtttaaat tctgtcggcc ccatctccag cggctcttcg cccgccatca ctcggtgaga
    38941 tgtcgggtcg agacttaatc cctgcatctc aatcacctct tccaccgcca ttggcgaaat
    39001 acggcgcatt accgctttga ttcgcgccac cagctccttc ggcgaaaatg gcttggtgat
    39061 atagtcatcc gcgccggttt caaggccgcg cacgcgatct tcttcttccc ctctggcggt
    39121 caacatcacc actggaatat cccgggtcat cgactcgcgc ttgaggtgtt tgatgaactg
    39181 gataccggag ccgccaagta acatccagtc aaggagaatt aaatccggcc aaggttcatt
    39241 cagttgattc acagcactgt cataatcttc cgcttcgacc ggctgaaagc cattttgttc
    39301 gagcacgaag cagaccattt cgcgaattgg agcttcatct tctacgacca gaatacgtct
    39361 cgccatgatt tgccctgttg taataaatag gttgcgatca ttaatgcgac gtcattatgc
    39421 gtcagattta tgacagattt atgaaaagct cgtcgcacat atcttcaggt tattgatttc
    39481 cgtggcgcag aaaaaagcaa atggcacatc tgtttgggta taatcgcgac catgcttttt
    39541 cgccagggaa ccgttatgcg catccttcac acctcagact ggcatctcgg ccagaacttc
    39601 tacagtaaaa gccgcgaagc tgaacatcag gcttttcttg actggctgct ggagacagca
    39661 caaacccatc aggtggatgc gattattgtt gctggtgatg ttttcgatac cggctcgccg
    39721 cccagttacg cccgcacgtt atacaaccgt tttgttgtca atttacagca aactggctgc
    39781 catctggtgg tactggcagg aaaccatgac tcggtcgcca cgctgaatga atcgcgcgat
    39841 atcatggcgt tcctcaatac aaccgtggtc gccagcgccg gacatgcgcc gcaaatcttg
    39901 cctcgtcgcg acgggacgcc aggcgcagtg ctgtgcccca ttccgttttt acgcccgcgt
    39961 gacattattt ccagccaggc ggggcttaac ggtattgaaa aacagcagca tttactggca
    40021 gcgattaccg attattacca acaacactat gccgatgcct gcaaactgcg cggcgatcag
    40081 cctctgccca tcatcgccac gggacattta acgaccgtgg gtgccagtaa aagtgacgcc
    40141 gtgcgtgaca tttatattgg cacgctggac gcgtttccgg cacaaaactt tccaccagcc
    40201 gactacatcg cgctcgggca tattcaccgc gcacagatta ttggcggcat ggaacatgtt
    40261 cgctattgcg gttcccccat tccactgagt tttgatgaat gcggtaagag taaatatgtc
    40321 catctggtga cattttcaaa cggcaaatta gagagcgtgg aaaacctgaa cgtaccggta
    40381 actcaaccca tggcggtgct gaaaggcgat ctggcgtcga ttaccgcaca gctggaacag
    40441 tggcgcgatg tatcgcagga gccacctgtc tggctggata tcgaaatcac tactgatgag
    40501 tatctgcatg atattcagcg caaaatccag gcattaaccg aatcattgcc tgtcgaagta
    40561 ttgctggtac gtcggagtcg tgaacagcgc gagcgtgtgt tagccagcca acagcgtgaa
    40621 accctcagcg aactcagcgt cgaagaggtg ttcaatcgcc gtctggcact ggaagaactg
    40681 gatgaatcgc agcagcaacg tctgcagcat cttttcacca cgacgttgca taccctcgcc
    40741 ggagaacacg aagcatgaaa attctcagcc tgcgcctgaa aaacctgaac tcattaaaag
    40801 gcgaatggaa gattgatttc acccgcgagc cgttcgccag caacgggctg tttgccatta
    40861 ccggcccaac cggtgcgggg aaaaccaccc tgctggacgc catttgtctg gcgctgtatc
    40921 acgaaactcc gcgtctctct aacgtttcac aatcgcaaaa tgatctcatg acccgcgata
    40981 ccgctgaatg tctggcggag gtggagtttg aagtgaaagg tgaagcgtat cgcgcgttct
    41041 ggagccagaa tcgggcgcgt aaccagccgg acggtaattt gcaggtgcct cgcgtagagc
    41101 tggcgcgctg cgccgacggc aaaatcctcg ccgacaaagt gaaagataag ctggaactga
    41161 cagcgacgtt aaccgggctg gattacgggc gcttcacccg ttcgatgctg ctttcgcagg
    41221 ggcaatttgc tgccttcctg aatgccaaac ccaaagaacg cgcggaattg ctcgaggagt
    41281 taaccggcac tgaaatctac gggcaaatct cggcgatggt ttttgagcag cacaaatcgg
    41341 cccgcactga gctggagaag ctgcaagcgc aggccagcgg cgtcacgttg ctcacgccgg
    41401 aacaagtgca atcgctgaca gcgagtttgc aggtacttac tgacgaagaa aaacagttaa
    41461 ttaccgcgca gcagcaagaa caacaatcgc taaactggtt aacgcgtcag gacgaattgc
    41521 agcaagaagc cagccgccgt cagcaggcct tgcaacaggc gttagccgaa gaagaaaaag
    41581 cgcaacctca actggcggcg cttagtctgg cacaaccggc acgaaatctt cgtccacact
    41641 gggaacgcat cgcagaacac agcgcggcgc tggcgcatat tcgccagcag attgaagaag
    41701 taaatactcg cttacagagc acaatggcgc ttcgcgcgag cattcgccac cacgcggcga
    41761 agcagtcagc agaattacag cagcagcaac aaagcctgaa tacctggtta caggaacacg
    41821 accgcttccg tcagtggaac aacgaactgg cgggttggcg tgcgcagttc tcccaacaaa
    41881 ccagcgatcg cgagcatctg cggcaatggc agcaacagtt aacccatgct gagcaaaaac
    41941 ttaatgcgct tgcggcgatc acgttgacgt taaccgccga tgaagttgct accgccctgg
    42001 cgcaacatgc cgagcaacgc ccactgcgtc agcgcctggt cgcgctgcat gggcagattg
    42061 ttccccaaca aaaacgtctg gcgcagttaa tggtcactat ccagaatgtc actctggaac
    42121 agacgcaacg taatgtcgca cttaacgaaa tgcgccagcg ttataaagaa aagacgcagc
    42181 aacttgccga tatgaaaacc atttgcgagc aggaagcgcg catcaaaacg ctggaagccc
    42241 agcgtgcaca gttacaggcg ggtcagcctt gtccactttg tggttccacc agccacccgg
    42301 cggtcgaggc gtatcaggcg ctggagcctg gtgttaatca gtctcgatta ctggcgctgg
    42361 aaaacgaagt taaaaagctc ggcgaagaag gtgcggcgct gcgcgggcaa ctggatgcat
    42421 taacgaagca gcttcagcgc gatgaaaacg aagcgcaaag cctccgacaa gatgagcaag
    42481 cacttactca acaatggcaa gccgtcacgg ccagcctcaa tatcaccttg cagccacagg
    42541 acgatattca accgtggctg gatgcacaag atgagcacga acgccagctg cggttactca
    42601 gccaacgaca tgaattacaa gggcagattg ccgcgcataa tcagcaaatt atccagtatc
    42661 aacagcaaat tgaacaacgt cagcagcagc tcttaacggc attggcgggt tatgcactga
    42721 cattgccaca ggaagatgaa gaagagagct ggttggcgac acgtcagcaa gaagcgcaga
    42781 gctggcagca acgccagaac gaattaaccg cgctgcaaaa ccgtattcag cagctgacgc
    42841 cgattctgga aacgttgccg caaagtgatg atctcccgca cagcgaagaa actgtggagc
    42901 tggataactg gcggcaggtt catgaacaat gtctcgcatt acacagccag cagcagacgt
    42961 tacagcaaca ggatgttctg gcggcgcaaa gtctgcaaaa agcccaagcg cagtttgaca
    43021 ccacgctaca ggccagcgtc tttgacgatc agcaggcgtt ccttgcggcg ctaatggatg
    43081 aacaaacact aacgcagctg gaacagctca agcagaatct ggaaaaccag cgccgtcagg
    43141 cgcaaacgct ggtcactcag acagcagaaa cgctggcaca gcatcaacaa caccgacctg
    43201 acgggttggc tctcactgtg acggtggagc agattcagca agagttagcg caaactcacc
    43261 aaaagttgcg tgaaaacacc acgagtcaag gcgagattcg ccagcagctg aagcaggatg
    43321 cagataaccg tcagcaacaa caaaccttaa tgcagcaaat tgctcaaatg acgcagcagg
    43381 ttgaggactg gggatatctg aattcgctaa taggttccaa agagggcgat aaattccgca
    43441 agtttgccca ggggctgacg ctggataatt tagtccatct cgctaatcag caacttaccc
    43501 ggctgcacgg gcgctatctg ttacagcgca aagccagcga ggcgctggaa gtcgaggttg
    43561 ttgatacctg gcaggcagat gcggtacgcg atacccgtac cctttccggc ggcgaaagtt
    43621 tcctcgttag tctggcgctg gcgctggcgc tttcggatct ggtcagccat aaaacacgta
    43681 ttgactcgct gttccttgat gaaggttttg gcacgctgga tagcgaaacg ctggataccg
    43741 cccttgatgc gctggatgcc ctgaacgcca gtggcaaaac catcggtgtg attagccacg
    43801 tagaagcgat gaaagagcgt attccggtgc agatcaaagt gaaaaagatc aacggcctgg
    43861 gctacagcaa actggaaagt acgtttgcag tgaaataact attcagcagg ataatgaata
    43921 cagaggggcg aattatctct tggccttgct ggtcgttatc ctgcaagcta tcactttatt
    43981 ggctacggtg attggtagcc gttctggtgg ttgtgatggt ggtatgaaaa aagtcatttt
    44041 atctttggct ctgggcacgt ttggtttggg gatagccgaa tttggcatta tgagcgtgct
    44101 cacggagctg gcgcataacg taggaatttc gattcctgct gccgggcata tgatctcgta
    44161 ttatgcactg gtggtggtgg tcggtgcgcc aatcatcgca ctcttttcca gccgctactc
    44221 actcaaacat atcttgttgt ttctggtggc gttgtgcgtc attggcaacg ccatgttcac
    44281 gctctcttcg tcttacctga tgctcgccat tggtcggctg gtatccggct ttccgcatgg
    44341 cgcatttttt ggcgtcggag cgatcgtgtt atcaaaaatt atcaaacccg gaaaagtcac
    44401 cgccgccgtg gcggggatgg tttccgggat gacagtcgcc aatttgctgg gcattccgct
    44461 gggaacgtat ttaagtcagg aatgctggcg ttacaccttt ttattgatcg ctgtttttaa
    44521 tattgcggtg atggcatcgg tctatttttg ggtgccagat attcgcgacg aggcgaaagg
    44581 aaatctgcgc gaacaatttc actttttgcg cagcccggcc ccgtggttaa ttttcgccgc
    44641 cacgatgttt ggcaacgcag gtgtgtttgc ctggttcagc tacgtaaagc catacatgat
    44701 gtttatttcc ggtttttcgg aaacggcgat gacctttatt atgatgttag ttgggctagg
    44761 gatggtgctg ggaaatatgc taagtggcag gatttcagga cgttattcac cactgcgcat
    44821 tgcagcagtg actgacttta tcattgtact ggcactgctg atgctctttt tctgcggcgg
    44881 catgaaaaca acgtcgctta tttttgcttt tatttgttgc gcgggattat ttgccctttc
    44941 agcacctctg caaatattgt tactgcaaaa tgccaaaggc ggagagttat taggtgccgc
    45001 aggtgggcaa atagcgttta acctcggtag cgccgtcggc gcatattgcg gaggtatgat
    45061 gctgacgctg gggctggcat ataattacgt ggcgctgcct gccgccctgt tttcgtttgc
    45121 tgcgatgtca tcgttgctgc tgtatggtcg ctataagcgc cagcaagcgg cggatagtcc
    45181 ggtgctggcg aaaccactgg ggtagattac tgtcacggtg cgcgttgctt attgccggat
    45241 gcggcgtgaa cgccttatcc gccctacgcg gttctggcac attttgtagg cctgataaga
    45301 cgcggcaagc gtcgcatcag gcatgatgcg ccaattgcct acgtttttta ctcttgtggc
    45361 cataaccacg cagcgccgcg tacgccgctg gaatcaccgt gcttcgcctt actcaccggc
    45421 gtttcacatt cgccgccgaa gacaaattgt ttaatcaact gcccaaccgt ttgatataaa
    45481 cggtctacat tgctcatccc gccccccagg acaatcacat ccggatcgag aatattcacg
    45541 acatgtgcca gcgattttgc cagccgcagc tcgtagcgac gcaatgccag ttccgctacc
    45601 ggatcgcttt cttcaaccag gcggataatt tcactgcctt tcagcgcatg tccgctcaaa
    45661 cgacgataat ccgtcgcgaa tcccgtgccc gaaataaagg tttcaataca accttgttta
    45721 ccgcaataac aagggacttc ctcgcgataa cgcagttcgt cttcgtccat ccacggtagc
    45781 ggattgtgtc cccactcacc tgccgtgcca ttgccgccga tatgcgtccg cccattgaat
    45841 gccacgcccg cgccgcatcc cgtgccgata atcacggcaa ataccgtctg cgctcccgct
    45901 gccgcgccat ctactgcttc tgaaaccgcc agacagttgg catcatttgc cagccgcact
    45961 tcccgctgca acctcgcgct taagtcttta tcgaatggct gaccgttgag ccaggttgaa
    46021 ttggcattct tcaccacacc ggtgtagggc gaaatcgaac ctggaatgcc catacctacc
    46081 gttccgcgct gccctgtcgc ctgctccgcc atatcaacca acgtggcgat cgtttcaata
    46141 gtctgccggt aatcatcacg cggcgtgggc agacgatggc ggtacaactg ctcccctgca
    46201 tcgcccagtg caatcacttc agttttggtg ccgcctaaat cgatacctat acgcactgta
    46261 ctctccttat ttttttcaat atcaatagcg tagagacgga caaccggatt ggcaatgcaa
    46321 ggccgcctac aattcgttat catgcccgct aaatttaacg acaaggccgt ggaaattatc
    46381 atgctgtggt tcaaaaattt aatggtttac cgtcttagcc gcgagatttc gctgcgtgca
    46441 gaagagatgg aaaaacagct agcctcgatg gcatttaccc catgcggcag ccaggacatg
    46501 gcgaagatgg gctgggttcc tccgatggga tcgcacagcg atgcgttaac gcacgttgcc
    46561 aatggtcaaa ttgttatctg cgcgcgcaaa gaagaaaaaa tcctcccgtc tccggtgatt
    46621 aaacaggcgc tggaagcgaa aatcgccaaa ctggaagcgg aacaggcgcg taagctgaag
    46681 aaaaccgaaa aagattcgct gaaagacgaa gtgctgcact ctctgctgcc gcgtgctttc
    46741 agccgtttta gccagacaat gatgtggctc gacacggtta acggtttgat tatggtggac
    46801 tgcgccagtg ccaaaaaagc ggaagatacg ctggcattac tgcgtaaaag cctggggtcg
    46861 ttaccggttg taccattgag catggaaaac ccgattgaac tgacgctgac cgaatgggtt
    46921 cgctccggta gtgcggcaca gggcttccag ctgcttgatg aagccgagct gaaatcgttg
    46981 ctggaagatg gcggcgtgat ccgcgcgaag aaacaagatc tgaccagcga agagatcacc
    47041 aatcacattg aagccggaaa agtggtgact aaactggcgc tcgactggca gcagcgcatt
    47101 cagtttgtga tgtgcgacga tggttcgctc aagcgtctta agttctgcga cgagctgcac
    47161 gatcaaaacg aagatatcga ccgtgaagat ttcgcccagc gttttgatgc cgatttcatc
    47221 ctgatgactg gtgaactggc agcgttaatt caaaacctga ttgaaggatt aggtggcgaa
    47281 gcacaacgtt aattgctgat tttcctttaa tgccggatgc gacgcctgcc gcgtcttatc
    47341 cggcgtacga agccacacca ggcatataat tattcgctac ggcgagcaat aatttttagc
    47401 gcagcaatat tatgcgtttt acgccgtaac tgatctaatc gcctcatccc tattcgcctg
    47461 ccagcagcac gcagaatatt cactaacccc catttatcgc tggtaaattg ttttgttaac
    47521 agctccgctt ttggggtatc aatatattca cgcaataaat cagaaattga acaaatatcc
    47581 gtcaggtaag gataccagca agtttcaccg tgaaaattac gcactccagc gttacctgca
    47641 ataaagtctt tcgggtaatc ccagatcacg tttttatcca acgtgatcca ataacgcggt
    47701 aaatcggtac ttccaccatt ttggctgcgc atgggataac gggtacagtg aatttgcagg
    47761 ttgatcgttg gcgtcagtaa gtcatagatc tcccgctgta acttgctcca tggacgttgt
    47821 gtcattgttt ttcctcaagc cggtgacgcg cttcactgcg aattttccgc gttgccccgg
    47881 ttttcactaa atcacgtaac tgcgaaagat cgggttgttc gacaaataag agtaatgatg
    47941 gatcctcttt taaccacgcc gttttcacat cggcagccag ctgtggatta ttaatagcca
    48001 gcgaccgatc ctgaggctca gtcaatgtcg ttaacaacga cgccggggta tgtgcgttgc
    48061 gtaatgccgt ttggcgcaaa tgatcattgc gactttgcag caatgcacgc caccattcat
    48121 catctgattt atcttttatc gtagccagtt ccgcaaaaaa tacctggag
//
LOCUS       DAETPZ010000024        44105 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.024, whole genome shotgun sequence.
ACCESSION   DAETPZ010000024 DAETPZ010000000
VERSION     DAETPZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.024"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1296..2978)
                     /gene="glcA"
                     /locus_tag="K0W59_001530"
     CDS             complement(1296..2978)
                     /gene="glcA"
                     /locus_tag="K0W59_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HBI3022624.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVAFAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFEPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
     gene            complement(3333..5504)
                     /gene="glcB"
                     /locus_tag="K0W59_001531"
     CDS             complement(3333..5504)
                     /gene="glcB"
                     /locus_tag="K0W59_001531"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HBI3022625.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLCELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMVPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5526..5930)
                     /locus_tag="K0W59_001532"
     CDS             complement(5526..5930)
                     /locus_tag="K0W59_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HBI3022626.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            complement(5956..7158)
                     /gene="glcF"
                     /locus_tag="K0W59_001533"
     CDS             complement(5956..7158)
                     /gene="glcF"
                     /locus_tag="K0W59_001533"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HBI3022627.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVENVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIV"
     gene            complement(7169..8221)
                     /gene="glcE"
                     /locus_tag="K0W59_001534"
     CDS             complement(7169..8221)
                     /gene="glcE"
                     /locus_tag="K0W59_001534"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HBI3022628.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(8221..9720)
                     /gene="glcD"
                     /locus_tag="K0W59_001535"
     CDS             complement(8221..9720)
                     /gene="glcD"
                     /locus_tag="K0W59_001535"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HBI3022629.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKESLDINPVGRRARVQPGVCNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNYILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            9966..10743
                     /gene="glcC"
                     /locus_tag="K0W59_001536"
                     /pseudo
     CDS             9966..10743
                     /gene="glcC"
                     /locus_tag="K0W59_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
     gene            complement(10867..12009)
                     /locus_tag="K0W59_001537"
                     /pseudo
     CDS             complement(10867..12009)
                     /locus_tag="K0W59_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            12413..14101
                     /locus_tag="K0W59_001538"
                     /pseudo
     CDS             12413..14101
                     /locus_tag="K0W59_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
     gene            14098..15012
                     /locus_tag="K0W59_001539"
     CDS             14098..15012
                     /locus_tag="K0W59_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBI3022630.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            15045..15293
                     /locus_tag="K0W59_001540"
     CDS             15045..15293
                     /locus_tag="K0W59_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBI3022631.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLNVKPPAQLMETLLPWLENK"
     gene            15293..16465
                     /locus_tag="K0W59_001541"
     CDS             15293..16465
                     /locus_tag="K0W59_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBI3022632.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFVVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISPGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(16501..17580)
                     /gene="lptG"
                     /locus_tag="K0W59_001542"
     CDS             complement(16501..17580)
                     /gene="lptG"
                     /locus_tag="K0W59_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBI3022633.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17577..18647)
                     /gene="lptF"
                     /locus_tag="K0W59_001543"
     CDS             complement(17577..18647)
                     /gene="lptF"
                     /locus_tag="K0W59_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBI3022634.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18678..19238)
                     /locus_tag="K0W59_001544"
     CDS             complement(18678..19238)
                     /locus_tag="K0W59_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBI3022635.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(19250..20074)
                     /locus_tag="K0W59_001545"
     CDS             complement(19250..20074)
                     /locus_tag="K0W59_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022636.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(20074..21423)
                     /locus_tag="K0W59_001546"
     CDS             complement(20074..21423)
                     /locus_tag="K0W59_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HBI3022637.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNIVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21469..22227)
                     /locus_tag="K0W59_001547"
     CDS             complement(21469..22227)
                     /locus_tag="K0W59_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022638.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(22259..22972)
                     /locus_tag="K0W59_001548"
     CDS             complement(22259..22972)
                     /locus_tag="K0W59_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBI3022639.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            23146..23838
                     /locus_tag="K0W59_001549"
     CDS             23146..23838
                     /locus_tag="K0W59_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBI3022640.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23887..25386)
                     /gene="pitB"
                     /locus_tag="K0W59_001550"
     CDS             complement(23887..25386)
                     /gene="pitB"
                     /locus_tag="K0W59_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HBI3022641.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGVNDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25678..27537)
                     /gene="gss"
                     /locus_tag="K0W59_001551"
     CDS             complement(25678..27537)
                     /gene="gss"
                     /locus_tag="K0W59_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HBI3022642.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVGHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27742..28608
                     /gene="yghU"
                     /locus_tag="K0W59_001552"
     CDS             27742..28608
                     /gene="yghU"
                     /locus_tag="K0W59_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HBI3022643.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGNEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28731..28979)
                     /gene="hybG"
                     /locus_tag="K0W59_001553"
     CDS             complement(28731..28979)
                     /gene="hybG"
                     /locus_tag="K0W59_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HBI3022644.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(29113..29454)
                     /gene="hypA"
                     /locus_tag="K0W59_001554"
     CDS             complement(29113..29454)
                     /gene="hypA"
                     /locus_tag="K0W59_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBI3022645.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29447..29935)
                     /gene="hybE"
                     /locus_tag="K0W59_001555"
     CDS             complement(29447..29935)
                     /gene="hybE"
                     /locus_tag="K0W59_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HBI3022646.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29928..30422)
                     /locus_tag="K0W59_001556"
     CDS             complement(29928..30422)
                     /locus_tag="K0W59_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HBI3022647.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALELVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30422..32125)
                     /gene="hybC"
                     /locus_tag="K0W59_001557"
     CDS             complement(30422..32125)
                     /gene="hybC"
                     /locus_tag="K0W59_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HBI3022648.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVSGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(32122..33300)
                     /gene="hybB"
                     /locus_tag="K0W59_001558"
     CDS             complement(32122..33300)
                     /gene="hybB"
                     /locus_tag="K0W59_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HBI3022649.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(33290..34276)
                     /gene="hybA"
                     /locus_tag="K0W59_001559"
     CDS             complement(33290..34276)
                     /gene="hybA"
                     /locus_tag="K0W59_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HBI3022650.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVMVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(34279..35397)
                     /gene="hybO"
                     /locus_tag="K0W59_001560"
     CDS             complement(34279..35397)
                     /gene="hybO"
                     /locus_tag="K0W59_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HBI3022651.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPYNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35586..35873)
                     /locus_tag="K0W59_001561"
     CDS             complement(35586..35873)
                     /locus_tag="K0W59_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HBI3022652.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35992..36879)
                     /locus_tag="K0W59_001562"
     CDS             complement(35992..36879)
                     /locus_tag="K0W59_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBI3022653.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            37036..38076
                     /gene="gpr"
                     /locus_tag="K0W59_001563"
     CDS             37036..38076
                     /gene="gpr"
                     /locus_tag="K0W59_001563"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HBI3022654.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKIRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(38116..38610)
                     /locus_tag="K0W59_001564"
     CDS             complement(38116..38610)
                     /locus_tag="K0W59_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HBI3022655.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38801..39685
                     /gene="yghA"
                     /locus_tag="K0W59_001565"
     CDS             38801..39685
                     /gene="yghA"
                     /locus_tag="K0W59_001565"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBI3022656.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     ARLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39957..40382)
                     /gene="exbD"
                     /locus_tag="K0W59_001566"
     CDS             complement(39957..40382)
                     /gene="exbD"
                     /locus_tag="K0W59_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HBI3022657.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40389..41123)
                     /gene="exbB"
                     /locus_tag="K0W59_001567"
     CDS             complement(40389..41123)
                     /gene="exbB"
                     /locus_tag="K0W59_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBI3022658.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41375..42562
                     /gene="metC"
                     /locus_tag="K0W59_001568"
     CDS             41375..42562
                     /gene="metC"
                     /locus_tag="K0W59_001568"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HBI3022659.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVVRVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42702..43361
                     /locus_tag="K0W59_001569"
     CDS             42702..43361
                     /locus_tag="K0W59_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI3022660.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43401..>44105)
                     /gene="yqhC"
                     /locus_tag="K0W59_001570"
     CDS             complement(43401..>44105)
                     /gene="yqhC"
                     /locus_tag="K0W59_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HBI3022661.1"
                     /translation="RVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLNVDILQLQ
                     ELLMDIGEDEHFQPSMAASGINSATLSEEILFAAERLLDVMERPLDARILGKQIIREI
                     LYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEANMSVSAFH
                     HNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSREFKRYFG
                     VTPGEDAARMRAMQGN"
BASE COUNT        10941 a        11762 c        10842 g        10560 t
ORIGIN      
        1 cgtaccgttt aaacaaagac aatacgtctc tttatttctc ataaaacccc aatcccttac
       61 agggcttatt gaaaaattta aaaatcacaa atttctcaat actcgaaatg agatcacata
      121 agaaatcaaa aagccttctc atctatttta agttttccca acggaaaata agagctttca
      181 ttaatactga accactttac tgttctattt gttcaggtat tgcattacgg tgtgctgaga
      241 tccatcggtg catccctgta tcccgcgatg agactaaaat agtcgtgatg taagcttgta
      301 tcagattctt agtgaatacc acatttgtta taattaagtg aaaagataca ttgctgattt
      361 acattatttg gtttacaaaa tgtcatcact tgcagagtta tcgctgacag atacctttaa
      421 tctcttacta ctttcgtgtt attgcaatac aaccatccgt aaagcagggc tatttttagc
      481 aggaaatact caggtgtcaa cattcgaact ccaactctga aatattcata taaattaatg
      541 attttttcat ttcttcgcgc ctaataaaag tattcaaatt cagatgattt ttctgctcaa
      601 cagacacaaa caggcaataa tactctccaa agcgaacaat atttggcatt acgatttaat
      661 gcttcataat caccaaatta tttaaccata aattttctta tttctaacaa aatgattggt
      721 gtgttgttgg agttaaaata catacataat attaacatct gccaacattt cactttccgc
      781 aagacggatg atttcgaatc ctcatccacg ttaccctatc ccaaatatct ttattaatag
      841 gcagtaacga acactgttat agcaagatta acttattaac ctaacggcat cttttatgta
      901 acaagtggag ataaattgct taaaattgat cctcgacaaa tcttaaaagt tctcattatt
      961 ctgcaaaatc agctttgcga ataatcttat gtttttggga gttttgggaa ttttgatcta
     1021 ctcattgttc tagtgaaaaa tataaataag agaaaaaacg ccattcgtca tgaaagcaac
     1081 agatgaagca atgtaataac atactaaaat tgatctcatg aaagtgggta ctttataacc
     1141 agcacagaag ccaagttgta taaatattaa acaattcgca tcgtgaaacg gagactacgt
     1201 ataaagaagc caatcgtaaa aacggttccc tggcgagata aacgacagac aaatggaaag
     1261 ccgggtgata tttaccttca tccggcttta ttgatttacg agactaacat cccggtaaac
     1321 acatacgcct gcagcagggt gataacgccg ataacgctgg caaaaatcag actgtgcttc
     1381 acggtgtagc ggaacagttc agattctcgg cccaccatgc ccgtcgcggc gcaggccacg
     1441 gcgatagatt gcggggagat catcttgcca gttacgccgc cgctggtatt tgctgccacc
     1501 agcagagtgt cagagacgtt gatttgctgc gccgtggtcg attgcagtga accaaacagg
     1561 gcgtttgagg aagtgtccga cccggtaagg aatacgccca gccagccgag aaacggtgag
     1621 aaaaacggga acatcacgcc tgtacctgcc agtaccagcg ccagcgtggt ggacatgccg
     1681 gaatagttgg tgacgaaggc gaatgccagc accatgccaa tcgacaatat cggccacttc
     1741 agactaatta gcgtttcggc aaagacgcca atccctttct tgatgcccac gccgaggatg
     1801 aagatagaga taatcgccgc aataaaaata gcggtgccgc cagccgagag aggttcgaat
     1861 ttaaacaccg catccattgg cgttggttgg gcgacaatgg gtgccgcttt caacacttgt
     1921 tgatgcaaat gagggatctg gaaattaatc accagtgaat aaaacgcgcc gcccggagca
     1981 aataacgctt taaacggctt catggtccag atggtgacca gcaccgttaa gattaaaaac
     2041 ggtgaccacg cacgaatgat ttgccccaga ctatattctg aaggcacggg accgccagaa
     2101 gatggcttat ttaccaccat cgcacctgcg gattgcccca tgctgattgc cgtttcggta
     2161 tttttcggcc gccagacttt aaggaataaa gcgagtgaga cgatactcac cagcgccgaa
     2221 gtaatatccg gcagttccgg accaatatag ttagaggtaa agaactgagt gacagcgaag
     2281 cttcccccag caaccagcgc cgctggccac gtctctttca cccctttcca gccgtccatc
     2341 attgctacca gccagaacgg cacaagaacc gacaggaacg gtaactgacg tcccgccatt
     2401 gcgccaatgt ggaacggatc gattcccgtt acctgaccgg cgaccagaat cggcacgccc
     2461 aacgcaccaa acgccaccgg cgcagtattg gcaatcagac acagccccgc cgcgtataac
     2521 ggtttgaagc ccaggcccac cagcagcgca ccggtaatcg ccaccggcgc accaaagcca
     2581 gccgctcctt ccagcaacgc accaaaggag aaaccaatca gtaacacctg caaacgctga
     2641 tcgtcggtga tggagataac cgagctgcgg ataatatcga actgcccgct ggcaacggtt
     2701 aatttataca ggaacaccgc cgcgacaata atccacgcaa ttggccataa tccataaata
     2761 aagccatagc ccgcagcagc aaatgccata tcaatcggca ttttaaaggc gaatatcgca
     2821 atcaggatag ataatataag ggttattgct ccagcgacat gtcctttcag acgtaatacc
     2881 gcgaatgcaa cgaagaagaa tattatcggg atcagggcga ccagagcgga tagccccagt
     2941 cctcccatcg gcatatacat ttgggtccag gtaaccatat tgttcagtct cttattatct
     3001 ttatatgctt gatatactta aggttgtaat aagcaaaaga ggactgaact gtaaaatata
     3061 ggcgttatac tttacagcaa cagtacgccg ctaacgcaat tgctacctct ggcataacaa
     3121 gtatatcggg taagggtttc tgttccgcac acgcaggcgc agagtatcgt taagatgtcc
     3181 atattgttgt tttaggcccg ctagtaatgc gctacgggta tttaatattg ttaaaccctg
     3241 ataatcgctc cggttatttc cgggataaat gtactaccgc agttactatc atagccccga
     3301 caataaaact tgccggggct tttttgacgc tattaatgac tttctttttc gcgtaaacgc
     3361 caggcgtgta ataacggttc ggtataaccg tttggctgtt tcacgccgag gaagattaaa
     3421 tcgctggcag ctttaaaagc acacgagtta gcgaaattcc ccgccatcgg acgataagcc
     3481 ggatcgccag cattttgctg atcaaccact ttcgccatat tctccagcga cgcctgcacc
     3541 tgttctttgg tcagaatacc gtgacgtaac cagttggcga tatgctggct ggagatacgc
     3601 agcgttgcgc ggtcttccat cagcgccacg ttgtgaatat ccggcacttt tgaacaacca
     3661 atcccctgct ccacccaacg caccacgtac cccagaatcc cctgcacgtt gttatccagc
     3721 tcttgttgga tctcttgcgc cgaccagtta gcgttttcag ccaccggaat agtcagcaga
     3781 tcgtccagca gcggttcaaa ttcagcattg aactcggtct gggcaatgtt ggcttgcacg
     3841 ctctgtacgt tggtttggtg gtagtgcagc gcatggagcg tagcagcggt tggtgacgga
     3901 acccaggctg tgtttgcccc ggcacgcagt tggtcgccct tctggctgta catgtctgcc
     3961 atcaggtccg gcattgccca catgccttta ccgatttgcg ctttaccgcg cagcccacag
     4021 aacagaccgg aaagcacgtt attacgttcg taggctttga tccacggcgt cgatttcatc
     4081 tggtttttac gcagcatcgg gccagcttcc atcaccgaat gcatttcatc gccggtacgg
     4141 tcgaggaaac cggtattgat gaacgccacg cggttgcgcg cctgagcgat acagctccgc
     4201 aagttcagcg aggtccggcg ttcttcatcc ataatgccca ttttcagggt attcggtacc
     4261 ataccgagca ttgtctcaat gcgggtaaac agtttgttgg cgaacgccac ttcctgcgga
     4321 ccgtgcattt tcggtttcac aatatagacg ctgccagtgc gcgagttttt ctgcactttt
     4381 aaatcataga gggcaatcgc gccagtcatg acgccatcaa gaataccttc cgggatttca
     4441 ttgccttcgc tgtcccaaat cacaggaatg gtcatcaaat gacccacgtt gcggataaac
     4501 agcaacgagc gtccgtgcag agaaatttca gagccatcgg cggcggtgta ctgacgatcg
     4561 tcattcagtt tacgtacgat ttgccgaccg tttttctcca ttttctcttg cagagtcccc
     4621 tgcatcaggc ccagcagatt gcggtacagc aggattttat cttccgcatc aaccgccgcg
     4681 accgaatctt cgcagtcgag aatggtactg atagcagctt cgacgataac atcgttgatg
     4741 tgcgccggat cgtctttgcc aatccgcccg ttggcatcaa tttgcagttc aatatgcagg
     4801 ccgttatttt tcagcaaaat gcaggtcggc gcagcggcat cgccacggta accgacaaac
     4861 tgtgctggag tacgtaacgt ggtttcttta ccatttttca actggatgcg taattgttta
     4921 tcgaccacct taaacgccac cacatcctga tagctgccgt tttccagcgg tagtgattca
     4981 tcgaggaaac gtcgaaccca ggcgataacc tgctcaccgc gctgcggatc gtagccactg
     5041 accatcgccc cttcctgcgg gatgatgtcg ctgccgtata acgcatcgta cagtgagccc
     5101 cagcgagcgt tcgccgcgtt cagcgcgtag cgggcgttca ttgccggaac caccagctgt
     5161 ggccccgcct gactggtgat ttcgctgtca atgcccgtgg tttccaccgt cacgcgctcc
     5221 ggttgcggca ccaggtagcc cagttcacaa aggaaagatt tataggccgc tttatctttt
     5281 accggccccg gattgctgcg atgccactca tcaagcgccg cctgaatgcg atcgcgttct
     5341 gccagcaact gacgattttc tggtgccaga tcgtgaacga tctcatcaac attgtgccag
     5401 aatgcagcag cgtccagttc cacgcccggc aaaacttctc catccacaaa acgtttaaaa
     5461 ttggcgtcaa tgcgtaaacg gccctgggtt atggtttgac tcattgtttc tctcctcgtt
     5521 ttcgcttatt tcgccaacac cgctgctgcc gctttcgcga cctgcgcatc ctgtgctccg
     5581 gttaaaccag aaacgcccac ggcaccaata atttgcccat caacaacaac cggtacgccg
     5641 ccttccagcg acgtcagtaa cggcgcagtc acgaacgcgg tacgtccgtt gttcaccatc
     5701 tcttcatagc ccttagtttc acgacgcccc agcgcggcgg tacgcgcttt ctcctgggag
     5761 atgtaagccg caatcggcgc gcaatcgtcc atgcgactta acgccagcag atgaccgccg
     5821 tcatcggcaa cagcaatgga aacagaccag ttatttttct gcgcctcttc ctgacctgcg
     5881 gcaataattg cactcgccat ttgctggcta agaatgactt tagttttcat ttttttattc
     5941 cttttcaagg gcttgttata caatttcaat ccagtgacgc acagaggtac gaccggcgct
     6001 cgccagatgc gtctggcaac caatgttggc ggtgacgatc atttccggtt tgccgctttc
     6061 cagcgcattc attttgttat cccgcagctg gcgtgccaga tcgggatgcg ttaacgcata
     6121 tgttcccgct gaaccgcagc acagatggct gtcgggaacg tccgttaagg taaatccaag
     6181 acgaagtaac acgttttcca cttcgccgtt cagcttttgc gcatgttgta gggtacacgg
     6241 gcagtggaag gccagttttt tatcgccgcg aattgccagt ttttccagcg gttcctcgcg
     6301 cagaagttcg actaaatcga ccgccagttc actgacctga cgcgctttat cggcatataa
     6361 cgcatcgttt ttcagcatct gcccatactc tttgacgaac gcgccgcaac cgctggcggt
     6421 ttgcaaaatt gcctcggcac ctgcttcaat cgcgggccac caggcatcaa tattattgcg
     6481 cgcccgtgcc aaccctttct cctgcgcatt aagatgatag tccaccgcgc cacaacagcc
     6541 tgcttcgtta gctgacatga cgctgatccc cagacgatcc agcactcgcg cagttgccgc
     6601 gttggtgttg ggcgaaagcg taggctgggc gcagccttcc agcattaaaa cccgacgctt
     6661 atggcgcagc ggcggacgcg gtttagcttt caccgtttca gcaggcagtt ttgctctgac
     6721 ctgttccggt aaaaacggtc gcagcaccag ccctacctgc gtcagcgcac ggaagaccgc
     6781 cggacgcggc actacctggc gcaatccttc gcgcagcatt cgctccggca gtgggcgttt
     6841 cactttctgc tcgacaatat cacgcccgat atccagcaaa ttgtgatagc gcacaccaga
     6901 aggacaggtg gtttcacaat tgcggcaagt gaggcagcga tcgagatgct cctgtgtttt
     6961 aagcgtgact tcgttgcctt ccagcacctg tttaatcaga tagatgcgcc cgcgcggccc
     7021 gtccagttca tcgcccagaa gctgataggt tgggcaggtt gcggtacaaa atccgcagtg
     7081 aacacaggcg cgcaggatgc tgtcggcttc cagcgcgcgc gcgttctgcc gcatctcttc
     7141 ggttaatcgg gtttgcatag cctgctcctc aaagttccgc gtacatgcga ccggggttaa
     7201 acacgccgca agggtcgagc tgctgtttaa gctgctggtg atagcggaat aaaggagccg
     7261 atagcggggc aaagccacca tctccggcac taaagcgggt cgcatgaccg ccagcgttgc
     7321 gggcgatgcg atggatttga ttgtcatcgg ctgtcgattt cagccagcgt aacgccccgc
     7381 cccagtcgat cagttgctcg ccgggtaaat ccatcatcgg cgcatcgctg ggtaatgaaa
     7441 tgcgccataa ggtacctggt aacgagaaga acggcagttg ttgttcacgc aattgctgcc
     7501 agaactgacc cgcaacctct tcgccaccca gcagttcacg cgctgctttt accgatcctt
     7561 cgccgccctc aaggcggatc cacaacgcat tgtcgaagta acataagcca ctaatgggta
     7621 atggctggag ttgccactcg gcgatttcac tcatggcttc ttgcaggctg atttcccgac
     7681 gcaggctcag agaggcacgc ggtcgcggta acactttcat tgagatttca gtgagcacgc
     7741 caagacaacc gtagcttccg gccattaacc gtgagagatc gtatccggca acgtttttca
     7801 tcacttcgcc accaaaacgc agatgttttc cagtgccggt aatgatgcgc gtgccgagga
     7861 caaaatcgcg gaccgaaccg ctccacgggc gacgcggccc cgccagcccg caggcgacca
     7921 tcccgcccca ggtggcttct tcaccataat gcggcggctc acagggaagc atttgcccgg
     7981 cgctctccag cgccgcttca atcgccacca gcggcgttcc ggcacgagcg gttatcacca
     8041 gctcggtcgg gtcgtaatta acaatgccgc gatgacaacg cacatccagc gtttgcccgg
     8101 tgactgggcg acctaaaaag gctttgctat tgctgccctg aatcaccagc ggcgttttat
     8161 cgctaattgc ctgattcacc tgctccagca gcgcctgact gtaatcacac tcgcgtagca
     8221 tcaaaagcgc tccagttcag gaaaaggtaa atgaccgtga tgcacatgca tggcaccaaa
     8281 ttcagcacag cggtgtagcg tgggaatgtt tttcccaggg ttcagcaaac catcggggtc
     8341 aaacgccgcc ttgaccgcat ggaaggtcgt gatttcatcg ctgttgaact gggcgcacat
     8401 ttgattgatt ttttctcgcc cgatgccatg ttccccactg atgctgccgc caacttcaac
     8461 gcagagttcg aggatcttcc cgcccagctc ttccgcgcgg gcaaattcac caggttcgtt
     8521 ggcatcgaaa aggattaacg ggtgcatgtt gccatctccg gcatgaaaga cgttggcaac
     8581 acgtaaatca tattgctgcg ataaacgggc aatgccttcc agtacgccag gcagggcgcg
     8641 acgcgggatg gtgccatcca tgcagtagta atccggggag atacgtccta ccgccgggaa
     8701 cgcatttttg cgaccggccc agaaacgtac gcgctctgct tcgtcctgtg ccaaacggac
     8761 gtcagtcgcg cccgctttca acaagatgta gttaacccgc tcgcagtctt cctgtacgtc
     8821 agactccacg ccgtccagtt cgcataacaa aatcgcttcg gcgtcgacgg gataaccggc
     8881 atgaataaaa tcttccgccg cgcggatcga caggttatcc atcatctcca gcccgccggg
     8941 gataatgcca ttggcgatga tgtcaccaac cgcaagtccg gctttttcta ccgagtcaaa
     9001 gctggctaac agaacccgcg ccacgggcgg cttcggcagc agttttaccg tcacttcggt
     9061 ggtcacgccg agcatacctt ccgatccggt gaacagcgcc agcaggtcaa aaccaggtga
     9121 atccagcgcg tccgatccca gcgtcagcgc ctcgccgtcc agcgtttgca cttcaatttt
     9181 cagcaggtta tgtacggtca gaccatattt caggcagtgg acgccaccgg cattttccgc
     9241 cacattgccg ccaattgaac aggcgatttg tgaggaagga tccggcgcgt aatagagatt
     9301 atgcggcgca acggcctggg agatcgccag gttacatacg cccggctgca cgcgcgcgcg
     9361 gcgaccaacg gggttaatgt cgaggctctc tttaaagcgc gccatcacca acaacacacc
     9421 tttttccagc ggcagcgcgc caccagaaag cccggtgcct gcaccacggg tcaccaccgg
     9481 tacacgcagg cgatggcaga cagccagaat cgctgtcacc tgttccattt gcttaggcag
     9541 aacaaccagt aatggacgcg tgcgatacgc gctcaacccg tcacactcgt aaggaatgat
     9601 ctcctcatcg gtatgcagga tctcaagtcc agggacatgc tcacgcagtg ccatcagtac
     9661 cgatgtgcgg tcgacatcgg gtaaagcgcc atcaagacgc tcttcgtaca agatgctcat
     9721 gagtaggctt cgctttgttg tgtggcagct gatttttgcg cgctgcttct gtgaacagtt
     9781 attaagcggg cttttcgttt tcgtctatct ctttagctac cggtcagacc attttttttc
     9841 cagctctgtg accttgtctt ggttaactta atgttaaatt gatgtaacat aatcacttgc
     9901 gtgatgtgcg tgttttgcga gttaagaaca gaaaaattgg tcctacctgt gcacgaggtc
     9961 cgggaatgaa agatgaacgt cgccctattt gcgaagtggt tgcagagagt atcgaacggt
    10021 taattatcga cggcgtactg aaggtcggtc agccgcttcc ctcggaacgt cgactgtgtg
    10081 aaaagctcgg cttctcacgc tccgcactgc gtgaagggct gaccgtgctg cgcgggcgcg
    10141 ggattattga aacggcgcag ggtcgcgatt ctcatgtcgc acggcttaat cgggtgcagg
    10201 acaccagccc gctgatccat ctgttcagta cgcagccgcg aacgctgtac gatctgctcg
    10261 acgttcgcgc attactggag ggcgaatcgg caaggctggc ggcaacgctg ggaacgcagg
    10321 ctgattttgt tgtgataacc cgctgttatg aaaaaatgct cgccgccagt gagaaccaca
    10381 aagagatttc gctgatcgaa catgcgcagt tggatcacgc tttccatctc gccatttgtc
    10441 aggcttctca caatcaggtg ctggtgttta cgctgcaatc attgaccgat ctgatgttta
    10501 attcagtgtt tgccagcgta aataatctct accatcgacc acagcaaaaa aagcagatcg
    10561 atcgccagca tgcgcggatc tacaacgcgg tgttgcagcg gctgccgcac gtcgcgcagc
    10621 gcgcagcgcg cagcgcgcga tcatgtacgg accgtgaaaa agaatctcca cgatatcgag
    10681 ctggaaggcc accatttgat tcgctcggcg gtgccgctgg agatgaacaa ggtgggaatg
    10741 tgagcagagt tttaccctat ccccaaggcg aggggaccgc ccctgcactt aacaggatct
    10801 ggtgttaaat ctgttcatct ataccctctc ccgaatgacc gcccctgcgc taaacaggat
    10861 ctggtattaa atttgctttt catacaatcg gtaacgcttg tacggctccg ccccaatgcg
    10921 ttccagcatg ttattcatgc ctgtattggt ttcgaggatc cattacatct ccagcgcatc
    10981 gatcttccgg cgggcaaacg gatcgcgtaa ggcttcaatc aataacagcg caatcaccgg
    11041 gccgatgcgg ctgaactgat actcgtcgcg cacgcccatc agcggtactc gcgcagttcg
    11101 cacaccgctg actttcaaac gccacagcaa ttttgcccag ccgaagggaa agagcgatcc
    11161 gttcagatcg gcaatcgcct cgttgatgtt aggcaagccg acaataaacg cgcagggtgc
    11221 agaatcaatc tcagcgatgt agatcatatc gtccggcacc agatatttaa gttggtcgcc
    11281 catggtcgcg aattcatgtt cggtaaacgg cacaaatccc cagttgtgct gccagccaga
    11341 gttgaaaatc tcacgcagga tctgcatctc ttcggcaaac cgctgacgat tgatgcagcg
    11401 aatggtcacc tttttgcgca cctgatccat ccgttttttt agcgccggag agaaagtgag
    11461 atcggttcgc tgcatccacc acgccagtaa atcaatgcct ttgtgataac ccagttgttc
    11521 aatatgcgcg gcataccacg gtttgccgtg tggcatcatc gcacagggtg gtgtgtcaaa
    11581 accttcaatc agtaatccgc tttcctgatt gatattcagg ctgaaaggcc cgcagatctt
    11641 acttgcacct tgtgacttca accacgcttc cgctgcgcca aacaacgcgg caaaatcctg
    11701 cggatcatca atggcgtcaa tcatgccgaa atgaccggta tcttcaccgt aacgctcacg
    11761 gtgcaaggta tctatttgcg cggtaatacg cccaactatc tgccccgctt tttttgccac
    11821 ccacgcctgc cagatgatat ggtccgtccc cggatttttc gcagacaaat gctcgttgcg
    11881 ttcaatgaat aaagggggta tccagtttgg atcgtcggga taaagtgatg acggaaaagc
    11941 gataaatgcc ttaaggtcat ttttattaat tactttttca attttaatca tcagcagatc
    12001 gcattccata tctctgtgga taacaaataa ttaatggtga aaaacaggtt gcaacagcaa
    12061 cgagcggcat cttaataatc aactcaatga atacaaacga cgaataaata gtattaatta
    12121 atgattatga taaggtcaga ggctacacag aaacgcctca atgccttttg tcgatttaag
    12181 gcgaccggac aaggcttctt cttaatatat tcataaatta aatatcattc ataataaaaa
    12241 taaaaaactc aaactcatta tttaaatcaa tgttcatttt cacaccgatg aaaatactat
    12301 ctcatttatc ttttatgcct atataatcca ccgcattatt gtcattttat ttttgatatc
    12361 caattttttc gtggtgtata tgtctaataa aatctttacg cattccctac ctatgcgcta
    12421 tgccgatttt ccaacgctgg ttgatgcttt ggactacgcc gctctgagta gagccggaat
    12481 gaatttttat gacagacgtt gccaacttga agatcaactg gaatatcaga cattaaaaac
    12541 gcgtgccgaa gctggtgcga agcggttgtt atcgctgaac ctgaaaaaag gcgaacgcgt
    12601 ggcgctgatt gccgaaacga gtagcgggtt tgtagaggct ttttttgcct gtcagtatgc
    12661 tggcttagtc gccttcccgt tggcgattcc aatgggcgtc ggtcagcggg attcctggag
    12721 cgccaaactc cagggtttac tggcaagttg ccagcccgca gccattatca ctggtgatga
    12781 gtagttgcca ctggtcaatg ccgcgacgca taacaacccc gaattacatg ttttaagcca
    12841 cgcttggttt aaggcattac cggaagccga tgttgcgctc cagcgtccag ttccgaacga
    12901 tatcgcctac ctccagtaca cctccggcag cacccgtttt ccccgtggcg tcattatcac
    12961 ccatcgcgaa gtgatggcta atctacgtgc tataagccac gatggcatta aattacgccc
    13021 tagcgaccgc tgcgtctcct ggcagccttt ctaccatgat atgggactgg tcggctttct
    13081 cctgcccccc gtcgccacgc aactttcagt agattatttg cgcactcagg attttgccat
    13141 gcgtcctttg caatggctta aattgatcag taaaaatcgc ggcaccgttt ccgttgcgcc
    13201 gccgtttggc tatgaattgt gccaacgccg cgtgaatgaa aaagatctcg ctgaactgga
    13261 tctttcctac tggcgcgtcg ctggtattgg tgctgagccg atctccgcag aacaactcca
    13321 tcaattcgct gaatgtttcc gtcaggttaa ctttgacaat aaaactttca tgccgtgcta
    13381 cggactggca gaaaatgcgc tggctgtcag cttctctgat gaagcctccg gggttgtggt
    13441 taacgaagtg gatcgcgaca tcctcgaata tcagggtaaa gccgtcgcgc cgggtgcaga
    13501 gacacgcgcc gtatcgactt tcgtcaactg cggcaaagcg ttgccggaac atggtattga
    13561 aatccgcaat gaagcaggta tgccggtcgc ggaacgtgtg gtaggccata tttgcatctc
    13621 cggtcccagt ctgatgagcg gttactttgg cgaccaggtt tcgcaagacg agattgccgc
    13681 gacgggctgg ttagacaccg gcgacctcgg ttatctgctg gacggttatc tgtatgtcac
    13741 cggacgcatt aaagatctga ttattattcg tggccgtaat atctggccgc aggatattga
    13801 atatatagcg gaacaggaac cggaaattca ttctggcgat gcgattgctt ttgttaccgc
    13861 ccaggaaaaa atcattttgc agatccagtg tcggatcagc gacgaagaac gtcgcgggca
    13921 gcttatccac gcgctggcgg cacggatcca aagcgaattt ggcgtgaccg cggctatcga
    13981 tctgttgccg ccccacagta ttccccgaac gtcctccggc aagcctgccc gtgcggaagc
    14041 gaaaaaacgt tatcagaagg cttatgctgc cagtcttaat gtgcaggaat ccctggcatg
    14101 aatcaaactg tcgcggtgac gggcgctacc gggtttatcg gtaaatatat tatcgataac
    14161 ctgctcgccc gcggctttca cgttcgcgca ttgacgcgta ctgcccgcgc tcacgtcaat
    14221 gataatctca cctgggtgcg cggttcgctg gaagatactc attcacttag cgagctggtt
    14281 gccggagcca gcgtagtggt ccattgcgcc gggcaagtgc gcgggcacaa agaagagatt
    14341 ttcacccgct gtaacgttga cggcagcctg cgcctgatgc aagcagcaaa agagagcggc
    14401 ttttgccaac gttttctgtt tatctcttcg ctggcggcgc gccatcccga gctctcctgg
    14461 tatgcaaaat ccaaatacgt cgccgagcaa cgactggcag caatggcgga cgaaattacg
    14521 ctgggcgttt ttcgcccgac agccgtgtat ggtcccggcg ataaagagtt aaaaccgctg
    14581 tttgactgga tgctacgcgg cctgctgcca cgacttggtg cgccagatac acaactctct
    14641 tttcttcacg tcaccgattt tgcgcaagca gtgggtcagt ggttaagcgc cgaaactgta
    14701 cagacgcaaa cctatgaatt atgcgatggc gtcgccggtg gctatgactg gcaacgcgta
    14761 cgacagcttg ccgccaaagc ccgttgtggt tccgtgcgaa tggttgacat tcctctgccg
    14821 gtactcacag gccttgcgga tatcagtacc gcgttgagtc gcctggcagg taaagaacct
    14881 atgctgaccc gctcgaaaat tcgtgaatta acccacgccg actggtcggc aagtaataac
    14941 cgtatttctg aagatatcaa ttggtttccc gggattagcc tggaacacgc attacgcaac
    15001 gggctatttt gattgtcttg ttttaaaggt gtgttaacgt ttctatggta aatcgtgaaa
    15061 tagtaatgga ttacatcctt tcctgtttac aggatttggt cgaaaacggt gtggaaatta
    15121 agcccgacag cgatctcgtc aacgatcttg gcctggagtc gatcaaagtg atggatctct
    15181 taatgatgct ggaagacaga ttcgatattt ccattcctat taatattctg ctgaatgtca
    15241 aaccccctgc gcaattaatg gaaactctac ttccatggct ggagaataaa taatggggct
    15301 atacgataaa tatgcgcgcc ttgctggcga gcgtctgcaa tttagtgaca acgggttaac
    15361 accgtttggc acctgtattg atgaagtgta ttccgccacc gaaggccgga tcggcaataa
    15421 aaaagtgatt ctggccggga ccaataatta tctcggttta acctttaatc atgatgccat
    15481 cagcgaaggt caggccgcgt tagcggccca aggcaccggc accaccggtt ctcgtatggc
    15541 aaacggcagt tatgcgccgc acctggcgtt agagaaagag attgccgagt ttttcaatcg
    15601 ccctaccgct atcgtttttt caaccggtta taccgctaat ttaggcgtca tcagcgcact
    15661 tgccgaccat aacgccgtgg tgttactgga tgccgacagc cacgccagta tttatgacgc
    15721 ctgttctctc ggtggcgcgg aaattattcg tttccgccat aacgatgcta aagatctgga
    15781 acggcgcatg gttcgcctcg gtgaacgggc gaaagaggcg attatcatcg ttgagggcat
    15841 ctacagcatg ctcggtgacg ttgcgccgct ggcggaaatt gttgatatca aacgtcgtct
    15901 cggtggttat ttgattgtcg atgaagcgca ttcgtttgtc gtgttaggcg caacggggcg
    15961 cggactggct gaagctgtcg gcgttgaaga tgatgtggat attatcgttg gtactttcag
    16021 taaaagtctg gcctcgattg gcggttttgc tgtcggttcg gaagcgatgg aagtgctgcg
    16081 ctacggcagc cgcccgtata tttttaccgc ctctccttct ccgtcctgca ttgcgaccgt
    16141 gcgctcgtcg ttaagaacta ttgccacaca accagagctg cggcaaaagc tgatggataa
    16201 cgccaaccat ctgtatgacg gtctgcaaaa actgggttat gagttaagtt cacatattag
    16261 cccggtagtg ccagtaataa tcggttcgaa agaggaaggt ttacgcatct ggcgcgagct
    16321 tatctcgccg ggcgtgtatg tgaatcttat cctgccgcct gccgcgcctg caggcatcac
    16381 gctgctgcgc tgtagcgtaa atgctgccca cagtcgtgag caaatcgatg ccatcattca
    16441 ggcatttgct actctgaagc agtaatgtga gcccgccgtg gtgtttgcgc cacggcgggt
    16501 ttaatgttgt ctgtcatacc aatacaccag gctcaacgcc accaacacaa acgctaccgg
    16561 taaaccgagc gcggtaacag gtgcgcttaa cgcaaatagc aatcccagat taaccatgat
    16621 ttgatagctg atccaggtga gtaagcccac gatgacgcct acagcgagac ggctacccat
    16681 cccaggcgaa cgcggggcac taaaggtaaa cggcacagcc agcaaaatca tcgccagggt
    16741 caggatgggt tgccccagtt tttcccacag agcaaggcgg tattcgctgc tgggttgccc
    16801 ggtttcctgc aaataatgga tgtaatgatt aagctgacgg acagaaaaag tattccccgg
    16861 cattgataac tcttcaagat tcatactggt gaaggccgat tgccaggcaa gattatctaa
    16921 tgtttccagc gtttctttgc cattcagcca ttttttatga ttcacaccat gcaggaccca
    16981 cgttttgtcg tcttcaatgg tggcactgcg tgcataaatg taggattcaa gcgagagatc
    17041 gtcgcgataa tgaaatatct ccacgcccac cggctggccc tgctcgttca gcgatttcac
    17101 tgtcacaaat tcattaccac gcctggccca cagcatattg ccggtaatgt catcgtcctc
    17161 ccccaacgcg gtggcggtcg atttgatttg caacgcctgt tgctgcaatg gcgacgccac
    17221 ccactcatct atcgcgccta acgaaacagt ccacaatatc ccggcaacca gcgccaccag
    17281 tgcaatacgg aagatagaaa accccgtgct gcgaatggcg gtaagctcac tgtttttcga
    17341 taactgcccc agtccaacaa taccacccaa taacgcgata aacggcgaaa gttcgaccag
    17401 tgtgcgcggt aaggtcatta gcaccaccag caccgcctga gtccagcgat aaccgcccgg
    17461 gctgacacca tccagttcgt taatcaggtt gaaggtggta aaaagcggca gcaatagccc
    17521 tgcagcggcg gcaaaaccga gaaagagatg acggattaaa tagcgactga aaacattcat
    17581 cgggaaaatt tctgcagcaa agagaagtca cgtgcgacca gtatcagcag gcccattagc
    17641 atgagtccgg gcactaacca caaaccagga atgaggggaa tcgcgccgtt agccaccagt
    17701 gtacggcaga cgtcgccgcc ataaaaaatg gcaataaaca gcaacgtcag tggcaataac
    17761 gttgaaaatc gcccttgccg cggttttacc cgacttaatg aaatggccag cagcgccatc
    17821 aacaatgcgg tcaggccacg gctttgtcgc cattgcagtt cggcatgatc ggcaggaaag
    17881 gcggagcgcg ccagctccgt gaccgatttc gctttgcgtt tgacgttcgg gctttgatcc
    17941 agcggattca gatgtaattg cagattacgg tagatctgtt cgttgtcgtc acgcccctga
    18001 tgatcgagaa gataggcagt gccgttgtgc aacataacgg tcggtttctc gggtgatggg
    18061 tcaaccacat caaccgaacg ggcgcggaaa atacgggttc gattggcagt agaagtataa
    18121 atcagcgcgt cagtcaggcg attattatcc tgatcaaccg tctgcgaaag gatcattcgt
    18181 ccgttatcgt tagtgttaaa tttctttgcc cgcaactggc gaacatccag ctccgactgc
    18241 gactgctgct ccagttgata aatctgcgca taagcccacg gacgaccata catcgacaga
    18301 agggtgacaa aaatacttag cggtatcgcc agataaagaa cggctttgta caagcgtccc
    18361 ggactgcccc ccgccgcaga gatagcggta atttccgagt cggtatacat ctgccctagc
    18421 gtcacgccaa ctgacacata cagaccaaca ggtaacaaca tctccagtgc aatcagtact
    18481 ttgtagaaaa cgatatccag cacaacatca agagctaatg tcccatttgc cgcttcggtc
    18541 agataacgct gtgcggagta gctggcgaaa ataaagatca gaaaaccgac gataatcagc
    18601 accagacggc gcgtaccacg catgatgtag tgttcaacca gcttcatctc actaacgccc
    18661 tcaatccttt gagtttttta ttcattgatc agtttctgcg ccaacgcaat gacatgatct
    18721 acctcttccg gcgtcaggct gccgtccttt gcccactgaa tttgcccggc tttattcagc
    18781 accagaatgg ttgaactctg ctccggcaag cgccaggcaa cgcgtccctg tccattgccg
    18841 tcaataacga attgcgccca cggatagcgg cgtttatttt tttcgatttt gccaacaaca
    18901 aaatagccag taccaaagat ggcatcatcg gtgttcacaa tggtggtggg ctgaaaacga
    18961 tcctgcggaa agtttgccgc ttcaaccgct ttaattaaca gtgagttctt tttcttcgcc
    19021 gacttacgcc cggcgatata ctgaataatg cggacttttc ccgctaattg tgtgctgttc
    19081 cacttttggt agtgaatttc gtcgtcgcta ttcagaagca gctcgccgcc ttcctggata
    19141 taaacagggg taaccgtttt gccagtgacg aaattgtgtg cctgcacacc cggggctaag
    19201 agcatgataa tcaacgcaat aatcaaacga gatgacatca ttgctccggc tatagcggaa
    19261 aacgcgaaat cagcagctct aagcgctgac aaatatcgct atccagcgcc aacggtgcat
    19321 aatacgactg gcggttttcc atatcatccg ccaccatgtc gtcgtgttta tctcccatca
    19381 ggccattaat ggcggcgagg aaacgggaaa tctgtaacgc ctctttattt tcaggatcaa
    19441 gcgatcttaa ggtgtcgtaa taagaaacct gctggctgat gatttttcgc acatcatgaa
    19501 attccctgcc ggttagtcgc ggtttggcaa taatctcttt cagggtatgc atttgctttt
    19561 gccgataggc aataaaactg cttacgcttt ctaactggct atggcgtaat gaatagttca
    19621 ccaacgacca gaccggagag cttaaataaa tccgtaacag gttgccgtaa tacgacacaa
    19681 tatttttctt accgttacga aagccgtcct gaaaacgccc cagaaaaacg gtcgttttgc
    19741 cgaataaaaa tccggcctga tgcttcttta aatacagccg ttgcgcaaaa cgcaggtgct
    19801 tgtagcgcgc tcgcatgtat ttaaattgtt tgcgctcatc agcagtcagc tcatcgcctt
    19861 tggcttgttt gcgaactaaa cgcagtaatt cctgacgatc aacgccattg agccagaatt
    19921 gcaggcataa cgcgtaatta tcatgaatga tttcggtggt acaacttagt gcaatagtcg
    19981 gcggcaaact aacctgtgca tcaacgacat catcttcttc aatcgcaaca aatagacgct
    20041 cgctcgcctc aagcgggaaa tattttctca tcatcaggat ttctctccgg ctgcgttata
    20101 aagcaaggaa tggcgctgaa aaaatgcctt taaggttgga attaacgaga gcgccacgtc
    20161 cagacattgc ccaccgaagt aagcaaacgc tgctcccagc acaccaaaca gatgacagaa
    20221 catgaacagc aatacgatgt agccgattga ggcgatggtt tgcgccacga gaaaagcgcg
    20281 ctgttttccc gccatcaata gcaatgactc ctgcgggaag cccagcatcg aaatcacaat
    20341 tgcgcccagc atcacctgaa tcaggtcata cgcttcgaga tacttaacgc caaacaccag
    20401 cgaaatgagc ggcttgccga cgatcaacac cgcgagcgcc accagaatac cgattccgcc
    20461 cgccagtaaa ccggacttca cacctaacaa ccacggtctg gtggtgcgcg gatctaaacg
    20521 catcacctct gggtaaaaac ttttacccag caaacccgct ggcgttccgg cggcgtcgaa
    20581 gaatgtcatg gcgattttaa ataacccagc ggcagcgggt cctaacacga tccccaccaa
    20641 cactgtgctg cacgagttac gcgccgacca gatggagtgg gcaatgttgg ttgaccagac
    20701 aaaactccac gcgcctttaa tgtgtcgggc agactcaaac agattcaatt tgaaggcgtt
    20761 atggatattt ctacggcgta attcgcgcgc ggcaaaccac cagtacatgg tgccgccaac
    20821 cagattcgac acgtaccagg caataacaaa acccgcaaaa ccaaagtcaa aataccaggc
    20881 tgcgacgctc cccgctgcgc gcagaaaagg tttcgtcgcc tgctgtacag caatcaaatc
    20941 aaagcgatct accgcacgca gaatgccggt cggcgtggag gaagccattg aaggaatgag
    21001 cgtgcaatac agcgccgcca gccaaaagct ttggtcatcc agacctaatg aatgggaaag
    21061 gaatggcagt aaggcaatgc cgccgacaat cgccaccgcg ccgctgacga tatccagcga
    21121 gaaggaaaat gagacgatat tgcgaaattg ctgcggatta ttgttggtca aggctggcgt
    21181 tccgtactga accaccagct gccatgtctg aaacttaata aaatcgctga tcgacttggc
    21241 gtacgattgc acaatcacca gtacaccaaa catggcgggc gtcatccctt taccggcaca
    21301 cgagagcgcc aacagcccca gcaaggcgct ctcgacatta ctggagccta accaggcgct
    21361 attgcgaata atggtgcgaa acgcgccatc tgcaaaccaa tgtttgatgt taaaacccgc
    21421 caattcagcc tgaccttatc gttgatagta aaaaatatcc cgccagcctt aagttaaact
    21481 tcggcggtca gaaacgatgg caaccagaga aaccgccttc tgtacctgtt ccagcacttc
    21541 gctgtagggc gctctggaat caatctcaag aatttttgtg ccgttatagc caatcgtcga
    21601 catgacgccg attttgtcct gcagctcggc atactcatgg tcaggcttgc gggagatggc
    21661 agtatcaata tcaatcccca aacgaatgat taattcagga cggtattgcg ccattttttg
    21721 gtataaactc cgttcgcgct gcgccagaaa catgctgatt ttcccggtcg cacgttcgac
    21781 gccgatcccc ggtccgtcat aataaaagcc cgaaatttca gcctgcggga agcgatcgct
    21841 gaccaccaga acgccacttt gcgccagtcg ctgaaccttg cgtagattcg ccattcttcg
    21901 cagcgagaag cagtacataa tcaccgccgc ccatagcgcc ggagatttgg ttttcatgct
    21961 ttgggttttc gaggatttgg ccgccagtcg ccgttccagc cagacgccaa ccaacggcaa
    22021 tcgtttgatt ttgtcgccgt cttcgccgga gagcagcccc agatagcggc gctcggtttg
    22081 ccagtgttgt tgcagcgatt tcaccaggtc ggtggtcagc gtggatttac cggtgccatc
    22141 acacccgacc accgcaatca gccccggaat gtagttgggc tgcggcgcgg ttgtactatt
    22201 aacagtttgt gtttgtagtg catccattcg ttaaatcatc ccctatccat tttggtgatc
    22261 aggaaagcgc ggcgtgaatt gcgcgtagtg acgcttgcat aatttcatcg gctggttgcc
    22321 gaccatccag ttcaaggatc ttggcgccat tgaatgtcag ttgcggcgta acggcgattt
    22381 tttcctgtaa cgctgccagt tggtggtcag gtttacgcgc aaacgcggtt tgttcatcaa
    22441 tgccaagacg aatcaacaat acgggtaaat aagacgccat ccattggtac agcttcaact
    22501 cgcgctgcct taacattttt atccaaccgt taccgcccgt ggtttttgcc aattgcgggc
    22561 catcaaagcg aaaacccggc acttcaacct gcgggtagcg gtcggtgatg agcagatagc
    22621 cttgctggct tttacacaac attttgcgaa acttgtacgc ccgccagcag gaaagcagat
    22681 agataaccag cgcagtaata ttgccaggcg gtgttgaggg cttttcgtgt acatgtgccg
    22741 ctttacttcg cagataacac ccacaaggtg cgccaataac agggagctgt gaaatccatt
    22801 cgccaattcg cccggacgat tgcccgagat aaatgtgttc tgttggcatt cttgctgcca
    22861 gttcatttac caggcttgcc gtgagggtcg atttacctga accatcacat cctacaatgg
    22921 caataacacg cactggcgtg gaattaatta ttgacatatt acgttgattc acgaaaaacc
    22981 cggcagtaaa taatgtattg aatattacgt ggtccgtgct cagaatatcc gttcaacctt
    23041 attgtgacaa tggttaattg tgacattgac ctgaattacg ctttcataaa aacatattaa
    23101 ccaaataaat atttttaatg gatatttaaa ttaaaggata tattcatgca gtcaataaca
    23161 cctccattaa ttgccgttat tggtagcgat ggttcaggca agtcaacggt gtgtgaacat
    23221 cttattaccg ttgtcgaaaa atatggtgct gccgaaagag ttcatttagg aaaacaggcc
    23281 ggaaatgtcg gtcgtgcagt gacaaaatta cctttgatgg gaaaatcctt acataaaaca
    23341 attgaacgaa atcaggtgaa aacagcaaaa aaattgcccg gaccagttcc ggcgctggta
    23401 attacagcgt ttgtcgcccg tcgcttactg cgctttcgtc atatgcttgc ctgtcgtcgt
    23461 cgcgggttaa ttgttctaac cgaccgttat cctcaggacc aaattcctgg cgcttacgat
    23521 ggtacggtgt tcccacctaa cgttgaaggt ggtcgttttg tctcatggct ggcaagccag
    23581 gaacgtaaag cgtttcactg gatggcgagc cataagcctg atctggtcat caaactcaat
    23641 gttgaccttg aagttgcctg tgcacgtaaa cccgaccata aacgggaatc gctggcgagg
    23701 aagattgcca taacgccaca gttaaccttt ggtggtgcac aactggttga tatcgatgcc
    23761 aatcagccac tggaacaggt gttggttgat gcagaaaaag cgattacgga ttttatgacc
    23821 gcgcgtggtt atcactagtc aaaaatggaa atgcccgatc gccaggaccg ggcattttca
    23881 ggaaggttaa atcaactgca atgctatcca gtacagccca ccagaaagaa aaattgccgc
    23941 cggtaaagta aacacccacg ccatcaggat gctggttacc gttttacgct gtaacccacc
    24001 gccgtccacc accatcgtcc ctgctaccgc agacgagagg acgtgtgttg tggagacagg
    24061 catcccaata taactggcaa ggccgataga cactgccgcc gtcatttgtg ccgccatgcc
    24121 ttgcgcatac gtcatgccgc gcttaccaat cttctcaccg atggtcatcg ctacacgacg
    24181 ccagccaatc atggtgccaa tgccgagcgc cagtgctacc gccatgatga tccacaccgg
    24241 agcgtactca atggtgctta acatatcgct gcgcagtttt ttcagcaggt tctggtcttc
    24301 tttactgacg cctggcagtt tcgccagctt cgcggaggta tcagagatgc acagcataat
    24361 gcggcgcagc tggctgcgct gactcacgct taacggctcg taactttcca tattgcctgg
    24421 cagcatcgtt ttaacgcgcg caatcgcatc aaaggtattt gccggatgac agtgaaactc
    24481 tgttacttgc gtgccatcag tcgatgctgc aggcaatgga ggttccatcg caatcaactt
    24541 ctgcggcagt tcaggatgct gttgcaggta gtgttcgaag ttggtaacgg catcgcgggt
    24601 acgggtaatt tcatagccgg acgcattcat attgacgacg aagccagcag gggcaatccc
    24661 caccagtacc agcattacca ggccgatccc tttctgcccg tcgttcacgc cgtgcgaaaa
    24721 cgccacgcct gcagcggaaa caatcagcgc aatacgcgtc cagaatggcg gtttacgttt
    24781 gccctttttc tttttgcgat cttccggaat gcggtgaata cggtcacgct ttttcgtccc
    24841 actccagaaa cggcgcagca ggaaaatcag accacccgca atgaccaggc cgacgatagg
    24901 ggaaacaatc agcgaggaga aaattttggt tacttcacgc aggtttaacg catccatcac
    24961 cgatgagccg gttaacagcg cgttggttaa accgatgccg ataatcgcac caatcaaggt
    25021 gtgcgaactg gaggccggta aaccaaagaa ccacgttccc aggttccaga taatcgccgc
    25081 cagcagcatg gaaaagacca tcgccagacc gtgagttgac cccatattca gcaacaaatc
    25141 ggttggtaac atatggacaa tggcataggc aacgctaagt ccgcccaata acacgccaaa
    25201 aaagttaaaa aatgccgcca tcaccacagc aagttgtggc tgcatggcac gagtataaat
    25261 aacggttgcc accgcattcg ccgtgtcatg aaaaccattg attgcttcgt agaacaacac
    25321 aaatgccaga gcaagtaata acaaaagccc cgtatatata tcaaggccaa caaataaatt
    25381 tagcatatat tagattacgc cattttgaat ttacgaacgg acgcattatc agtgacttta
    25441 acggcatggg caaagtgaaa tatcattttt taacgataaa aagagtgtta tttcttgtgt
    25501 attagacaat aagcattcat tcaaaacctt tagaaataat caaaacacag gtattccact
    25561 ggttaatata ttcacatatg aaatgaataa atattggaat atataaatat tgaatatttt
    25621 gattaatccc cgtactaatt attcttcata atcagtacgg ggttgctaca acatgtatta
    25681 ctttttcacc acaattaacg gttcaatatc actctctttt ttgatgacca gtgattcatc
    25741 accgcgcaaa cacgtcccac cgtagttgcc gccaacggtg aaggtacata cctgaatgta
    25801 tttaccgtcc actttcggca aacaccacag ttgctgatag atgtttttct gctcggcaaa
    25861 tttaccgctg gttttgtcca gcacctcttc atgatggccg accaggtcga tattgctacc
    25921 acagcgaccg gcgatcggtt tcactgcgta acctgttttc accaattcat cattaacagt
    25981 gaaatcggta tccagcaggt aacgatggtg cgggaacagc gaccagagga tcggcagaat
    26041 cgctttgttg ccggggatca ccgtccacag cggctcaaag accagcactt ccgggcgcag
    26101 caatacgtcg ataagacgca cttcgttttg cggatgaccg gtacggattg gcaccgcagc
    26161 aaactcacgg tcgctaactt cacgaatctg atcaaacgcg gtttcccacg cccaggtttt
    26221 ccacacgcag ttaaccagtc gcccttcccc atcaatcagt tgcccggcag catcccagcc
    26281 cagttcatcc agcccgcgca agatacgcgt ttcaaagccc gcctggtgca gcgcctgctc
    26341 cataaactgc gcgtgatagt tttcctcgat atctttgtcc tgcatgatat ggacaaacgg
    26401 acgtgcacga ctgtgtttcc aggcaccagc cagttcgtta atcagccctt ccgccggatt
    26461 gaagccgttg cctttatagc cctgctccgc ccaacgttcg aggatcaagc ccgcttcggt
    26521 atgacaggag gcggagtcgg cgttgtattc gtaaaccttc aggccacgct catccatgca
    26581 gaaatccata cgaccagtga tcatatggtg acggcgacgc tgccaggaga gacgcaaacg
    26641 tggccagagg attttcggaa tgtcgaacag cgccagcagg ttgtcatctt tcagcacctt
    26701 gtcggttgcg tgaagataca tcagatgcag ctcgttggtg gctttaatta gctcctgctc
    26761 ggcactctcg gtaatggtgt agtaatgata cggatcctga ttgatcacct gaccgttggc
    26821 ctgcacatag gcgttttgta gcggatcttt ttcatccagc catttaccgt caaactggcc
    26881 tttgttttcc agacgcgctc cactgatttt cagcagctcg cctgcaattt ccggctgcgg
    26941 taagctgtat tcagtatctt ccgtctggat catccagccc agaatggtgg tgtcatcaaa
    27001 agtgtcttta agggtatagc agccgttttc gaccaccatc tccagctcgc gcgtccactg
    27061 ttgcccttgc ggcaacgggg aatgaatcac gttctgttcc gcaatacgga ctttgttgcc
    27121 atgcaattgg gtaatgatgg cgacatggcc agtgtcttta aattcaccgc ctttatccca
    27181 gataagaagc gcacccgcga ccggcgcacg cggcgagccg ttaggaaatg cctgcaatgg
    27241 caggatgttg tcattaacca cttctcgcag gaagcgcagc gagaaaatct cccacgccat
    27301 acccacgtca gtaaagacca caccgtaatt cagaaagaga aaacggcgag caaattcaac
    27361 gcattgccac ttgtggccca tatattcgtc gtcgatatag ctacggaata cggcgtcatc
    27421 ttcgtattcc tgcggatcga gagaactgta atctgaagag tagattgcta ccccacctgg
    27481 ggcgtagccc aataatgtcc cgaacggggc atcctggctg gtcgttcctt tgctcatcac
    27541 tttaccttta acaatacaac ctaagcagtt ggcgtaattg ttgtgctctg attacctgct
    27601 cagcgacatt aaccggacag aggtcagact aatcatacac ctcatcgcag cggctggcgc
    27661 aaagggttaa aaaattcaca ttctgtacag caagtgacct gctacactgc ttcaacacta
    27721 ccactcagaa ggcaactcac tatgacagac aatacttatc agcccgcgaa agtctggacg
    27781 tgggataaat ccgctggcgg cgcgttcgcc aatatcaatc gcccgttttc tggtccgacg
    27841 catgaaaaaa cgctgcccgt tggcaaacat ccattgcaac tttattcgct gggaacgccg
    27901 aacggtcaga aagtaacgat tatgcttgag gagctactgg cgctgggcgt tactggtgca
    27961 gagtacgacg cctggctgat tcgtattggc gatggcgatc aattctccag cggctttgtc
    28021 gaagtgaacc caaactcgaa gatcccggcg ctgcgcgatc atacgcataa tccgccgatc
    28081 cgcgtgtttg aatctggttc gatcctgctt tatctggcgg agaaatttgg ctacttcctg
    28141 ccgcaggatc tggcaaaacg gactgaaacg ttgaactggc tgttctggtt acagggtgcc
    28201 gcgccgttcc tcggcggtgg ttttggtcac ttttaccatt acgcgccggt gaaaattgag
    28261 tacgccatca accgctttac catggaagcc aagcgcctgc tcgacgtgct ggataagcaa
    28321 ctggcgcagc ataagtttgt tgcgggcaat gagtacacca ttgcggatat ggcgatttgg
    28381 ccgtggtttg gcaacgtggt gctcggcggc gtgtatgatg ccgcagagtt tctggatgcg
    28441 ggaagttata aacatgtgca acgctgggcg aaagaagtgg gcgaacgtcc ggcagtgaaa
    28501 cgtgggcgta ttgttaaccg caccaacggg ccgctgaacg agcagttgca tgagcgccat
    28561 gacgccagtg attttgagac aaatacagaa gataagcgtc aggggtaagg gttggtgttc
    28621 gtcgcagcaa gccatccagg ccggataagg cgttcacgcc gcatccggca atcgtgcaca
    28681 atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aagctaatca tcacgcgctg
    28741 gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg ctttggcttc atcttcgtcg
    28801 atgatgctca tggcaaatcc gacgtgtacc agcacccact ggcccagcag atcggcaggg
    28861 ttaccttcgc aaatcagggc gatattcacg tcgcgcttga taccacatac ttcaacctgc
    28921 gcaagctggt gaatatcttc accgacagcc agcacctggc ctggaacgcc aatacacatt
    28981 gttaactccg gttatttcga ttgccggaca aacgcgaaaa agaaccttta gcgggtgttc
    29041 cctctcaccc taaccctctc cccggagggg cgaggggacc gtccgtgcgc tttttaggcg
    29101 ttgtcctcag tcttattcaa cttcaatact tttgacgatc agcgaatcgc cggtatcgac
    29161 acgcaaccgc tcgccgtgac agagcggaca ctgcgcatcg tgctgatgaa tctccaccac
    29221 ctggctgcaa tcccagcacc aggcctgggc gggtttatag acgatatgta aatcgcaccc
    29281 ttgcgccacc gttccgtggc agacaatttc aaaactaaaa cggacggcgc tctcctcaac
    29341 gcaggagagc gcgccaattt ccagccacac ggcggtgacg cgcttaacat cgtgctgctc
    29401 cgcctgccgt tggataattt caacggcgct ctggcaaagc gacaactcat gcattttcgc
    29461 ccctcctgcg gccaaacagc agggcgcgac gccctgcgtg tggtacatcc ggattggtga
    29521 ctggcaacga aaggatcatt cgtgcgcagt catccgtcag gcgttggcct tcttcaatcg
    29581 acatgctgtg cgaaagcggc gacatcagcg aacaggagag gtattgcgaa acaccgtcca
    29641 gttcgccaac ggtaaaggtc atggtgccat acggcagttg cagaccaatt ttttcactca
    29701 ctttgcgcag cggccagagt tgatccgggc cggggaagat aactgcactc agcatccacg
    29761 gggtgatcac acaccccgtc cactgacctt cgaacagcgt aaaatcagaa acatacaccg
    29821 gcattgaagg atgcagaaaa gagagatcgt gcatcgaacg ccgggcaatt tcttcaaacg
    29881 ctgcctgtac ttgcgccttc ggggaggtct ggaaacctgc tatctcttca gtcatgaatc
    29941 gcctcccgtg ggatggcttc cacgcctgat tcgcgcagcg cagccagaac cagctcaagc
    30001 gcaggttcaa tcattgcttc aaccgtcggc gttaagccga tgtgtggctc cagcgattcc
    30061 gggatcacgc cgaccagggt cagctttttc ggaaactcac cggtgaagcg cagggccgac
    30121 aatacgtcgg ccaggccaag ctgatgcgga gagattttgt tggtaaacaa cgccggaact
    30181 tcttcatccc gcaggatcat catcgttccc ggcgtgttct ttttcgacac aatggcatcc
    30241 gcaataatca gatgatcgcg atttgccatg tcgccaagca gctccattcc cgccgtgccg
    30301 ccatcgagga tctcaacata atccggcaga atgtatcgtt gctctaacgc ttcgacaatc
    30361 cgcacgccga tggcttcatc ggtcagcaaa atattgccga cccctaagac taaaatacgc
    30421 attacagaac cttcactgaa accacttcgt tgccgtcggc atccactacg tgtaccgcac
    30481 aggccatgca cgggtcgaag gagtgaatgg tacgcaccac ttccagcggt ttattcggat
    30541 cggcaaccgg tgtacccacc agcgactgct cgtaaggacc gacgtcatca ttgaagttac
    30601 gcggaccaga gttccaggtt gatggaacaa ccgcctggta gttgctgatg ataccgtctt
    30661 taataaccat ccagtgagag agcataccgc gcggcgcttc gaggaagcca acacctttga
    30721 actcacccgt tgccggaatg ttcggcttca caaaggtggt gtgatcgcct ttgccgatat
    30781 tggtgatcag tgcactgtat tggttttgca ggatatcctg caattcacag cagtgaacgg
    30841 tacgaccaat aatacggccc agcgtggagt gcagctgtgc cacttccagc gtgttgccag
    30901 tcagtttctg ataaatcgca acgatttcat tcagtttgtt ctgggtagat tcgcgacctg
    30961 ccgccagttt caccagcata ttagccagcg gacccacttc taccgttttg ccgtagaaag
    31021 tcggtgattt cacccaggaa tatttcccgt cgtcagacca accatcataa gccggaatgg
    31081 tggtaccttc ccatggcgcc tgtggcgctt cgtctttata ccaggagtgc ttcgcgcttt
    31141 cctgaatgcc tttgatcagg tattcatcgg aatgagaagt gatcggacga tacgaggaca
    31201 gatccgcatt ctcaatgtag ccgcccggga acaggaagct gccgtttttg ctgtcggtcg
    31261 ggaattccgg cacgctcagg tagttcaccg cacctttacc gcgcgtcagc cattccgggt
    31321 agaacgcggc gataaccgcg gtatcaacct tataaacctg ctcaacaaag tcgctcagtt
    31381 tgtcgatgaa agacttgatg tacatcaggc gctcaaggtt caacacgccc aacccgtcga
    31441 ggttgattgg gttcgcgaca ccactcactg ccaggttctg aatgtgcggc gttttaccgc
    31501 ccagcagcgc cacgacgcgg ttagcgtcac gctggcactc cagcgcctgc aggtagtgcg
    31561 ctaccgcaat caggttcact tccggcggca gtttcatcgc cgggtgaccc cagtagccat
    31621 tggcgaaaat acccaactga ccgctggcaa ccagatcttt gatcttgttc tgaactttag
    31681 tgaactcttc cggactgttc agatgccagg tcgaaacgcc tttcagcatt tcggaggctt
    31741 tggttgggtc agcttgcagt gcagaagtga tgtccaccca gtccagcgcc gaaagctgat
    31801 agaaatgaac gatatggtca tgcgtggtgt gcgcagccag aatgatgtta cggatgtatt
    31861 gcgcgttaac cggaacgtcg atattcagcg cactttctgc cgcacgaacg gaagacagcg
    31921 cgtgagtggt agtacatacg ccacagatac gttgcacaat catccatgca tcgcgcggat
    31981 cgcggttttt cacgatctct tccatgccgc gccacatggt accggaagcc catgctttcg
    32041 aaacgacgcc attttcgatt tcgcaatcga tgcgtaaatg cccctcaata cgggttaccg
    32101 gatcaatagt aattctctgg ctcatgcttt gctcgcctca tgacgattat gatcgttttg
    32161 ttttaaagga ggaagtatcg gcagtagacg aatgagtacg atgtaagcgc aaatctcaat
    32221 agccacaaaa ccaatagaaa tcaacagttc ttcccaggtc gggaagtagg cgtaaccgcc
    32281 gcccgggttg aatgccacca acgaataggt cagacgccag gttgcacaac ctaacagtgc
    32341 gctcagtgct gacaggaaca gcatgcggga atcattacgc agcttcgcca cacgcagaac
    32401 gaccagcggg aagagcatca gcaggacttc aatccagaac atcacggagt agaagtcacc
    32461 ggcaaacgct aacgacagct tgtcgcgata gataagctcg ccaaagcgca gcccgatgaa
    32521 aatcgccagc aacacactga tggtgttggt cagcttgacg aacaggctct tttcatccgg
    32581 accgttgcca cgcagacccg cctgcaccag tgaaccttca aagatgacaa tcgagaagcc
    32641 catgatgaac gccgtcagca gcgagaacag cggcaacatt tcatagctct gccagagcgg
    32701 atgcaccttg tagcccgccg agatcatcag cgaccccatt gaagactggt gcatggtcgg
    32761 cagcagcgca ccgagcgcga tgatgaagaa catcaccttg ttcagacgtt gtagcgacac
    32821 cttccagccc agacgttcaa acagtgccgg agcaaactcc agagccatca cgccgatgta
    32881 gatggtcata cagaccgccg tctcgaacag taccgagttc acgttgaagt gacccggaat
    32941 gtagaagtac ggcaggttcc agtagcgacc cacgtcgata gtgatcgaca agccacccag
    33001 tgagtaacca aacagactcg ccaacagcgc cggacgcacc agcggatggt attgcccacg
    33061 gttaaagacg tataccgccc acgccagcgc ccagccgcca caggcaaagc cggtgccaat
    33121 cagcaggtca aacgcgatcc acacgcccca cgggaagccg ccgttcaggt cagagacaga
    33181 gcccagaccg aacaccagac gcttcacaat caggagcata cagatgacga ttaacggtcc
    33241 aaaaatcatg accggtttac tgatgatttt gccgcccagc ggttgtggat catgactcat
    33301 gatcgtctcc tccgtcgtga tggtcgtttt tggtgttgcg acgaaccagc acggttaagc
    33361 ccgccagcac agccagtggt agcatcatgc ctttatacag ggtgtgttga atattttcgg
    33421 aacgcgcacc ggtagaaaga tcgtccagtt tcggcaggtc gagattttca taaggcacac
    33481 ccgtcagtac cataacctga gtaccgccgc cctctttctc gccgtacaga tgcggataat
    33541 atttcggcac cgtatgcagg taagtgtcgc cagatttcag cgtctgacgc ggatagtggt
    33601 attcgctgcc aggcttcagc gccagacgtt ttttcgcctc cgccatcagc tcttcacgcg
    33661 taccgaaaat caccgcgccc gccgggcaca cttctacgca gccaggcaga ccgcctttat
    33721 cgagacgttc cacacctttc tggttgcaca gctcgcactt atgcagcgca ccaaacgggt
    33781 tgttgtagtc gtacttcggc acgttgtacg gacaggcgac catgcagtaa cggcaaccgg
    33841 tgcacacgtc tttgtcgtaa tggacaatgc cggttttcgg atcttttttc agtgcagaga
    33901 ccgggcacac agagacacag ttcggatcga cgcagtgcat acactgtttc ttaatgtacg
    33961 cgtagccgtt ctcctcctgg tctttgttga cccctgtgcc gctggtccac acctgaatga
    34021 tgttattggt atacggcgac agtttgtcgt tgttcgacca ggtctgttcc ccttgcgggt
    34081 tacgttcagg gaagttgata tcctgacact tggtgacgca agcctggcag cctacgcaca
    34141 aggtcgagtc gtacaacatc cccagcgatc ccggaattgg cgggcggttt tcagcagccg
    34201 catgactgac agacggcagc gcgcccgtca gcaatgcccc gcaggaggct gctttaataa
    34261 aattacgtct gttcacggtt attctccccg tgagtcagcg ttatctttct tttgctgacg
    34321 acccagttca cgcaccgcca tcacgctgac accggcaacc agcccaacca caccgccgag
    34381 caaaccaata gcgcctgcag agacgttgcc gccctcttta gcgttaacat ccggtttctg
    34441 tgaacgcgga gtctgatttt cgacgttggc aagctgatgg atgcctttat ggaagccgat
    34501 acctttttcg ttacagccat agcacgggtg accaatcgcc accggccaca caccgccaac
    34561 atcgcagaat tgcagcgttg agcagttgcc gtaagtttct ggccctttac agccgaggtg
    34621 gtacaggcac cagccttcgc ggtggccttc atcaccgaac tctttggcaa aacgaccagc
    34681 atcgaagtgc gggcgacgtt cgcagtgttc gtgaatcaga cggccatagg cgaaggtcgg
    34741 acggtttttg tcatccagtt tcggcggttt gccgtaagtg atgatgtgcg caacggtcgc
    34801 gaggaagttg tacgggttcg gcgggcagcc cggaatattg ataacggttt tgcctggcag
    34861 aacttcttgc aagctgactg cgccagttgg gttaactcca gctgcggcaa caccgcccca
    34921 cgcggagcag gaaccaatag cgataatggc tgctgcgcct tccgccgctt tgcggatgtg
    34981 atccacaatc ggctcaccgg caaccatgca ataaataccg ttatctttta atgggatgga
    35041 accatccacc actaacacat actgcccttt gtacttctcg agagcgttat gtttgttctc
    35101 ttcgacctga tgaccgaagg cggcggaaag cacttcgtga tattccagag agatagtctc
    35161 cagcacgagg ttttctaccg ttggatgcgt tgcacgaagc agagattccg tacaaccggt
    35221 gcactcctgc gcgccaatcc agataactgg cggacgctgc gggttagtaa ccgattcggc
    35281 catctctgca gcggctttgc tacttaaccc catggtggcg gctaatgctg cacaaagctt
    35341 catgaaatca cgacggttaa tgccgtgaga atggatgagg gtgttatctc cagtcattta
    35401 tagttattcc gttgcgagga cctggcatat attttgcttc aatcgcaaaa tcaataatgc
    35461 gatcgatgcg ccatttacca cacatttatt atggttatcg atatcatgat actgcggcgg
    35521 ggcgctaaaa cgaagggaag aagcattagt gtaattaatt aaagcaagat aatacgtatg
    35581 tttgatcaat tttcgaggcg ataaccggcc ataaaaaagc gcaacgtaga acaggaatta
    35641 ttctcacgaa agaaatccat taccatctct ttatccagtc catagcggcg cgtcagaata
    35701 cccgcttccc aggcgctaag ctggcgatct ccggtctttt cgtacatccg gtgtgagtag
    35761 cctaatacaa aaccgcgttt ataatcggca cagaattttg tcacattaac cgcactgtcg
    35821 gcatgcgttg cttttaatcc cgccattaag cctttaccaa aatggttatt catcgttcac
    35881 cccattcgtt atatatcgcc atatatagcg atattttagg caaagaacga cttttgcgca
    35941 cgctattttt tcatacttgt ttaagacgta atcccgtaag agataaacct atcaggagag
    36001 atatttatcg aaccatttca gtgtcctttg ccaggcaaga tcggcggcag atttgtcata
    36061 acggggcgtg gaatcattat ggaatccgtg attaaccccc ggatagatat aagcctcata
    36121 aaccttatta ttgtctttca acgccgcctc gtaaggaggc cagccctcgt tgattcgggt
    36181 gtccagttcc gcgaagtgga gtagtaaagg cgcttcaatc ttcgccacat cggcagtggg
    36241 tgcctgacga ccataaaacg gcaccgcgca ggccagttcc ggatacgcga cagccgccgc
    36301 gttcgatacg ccaccgccat agcaaaatcc ggtaataccc actttacctg tcgcttgcgg
    36361 atagcgttgc ataaactcaa ttgcggcaaa gaaatcattc atcagtttgg ttggatcaac
    36421 ctgctgttgc agctcacgac ctttatcatc atttcccgga taacctccaa cggaacttaa
    36481 tccgtcaggt gccagggcga tataccccgc cttcgccact cgccgtgcca catcttcgat
    36541 atacggattc agtccacgat tctcatgcac cacccccacg gctggcgttt tgccgctcat
    36601 ttttgcgggt ttcaccagat aaccccgtac ctcgccgtga ccatttggcg aaggatacgt
    36661 gatgtactca gcaacaatct ccgggtcggt aaactctacc tgagtcgcca gcgcataatt
    36721 tggcttgagc aaatcaaaca acgctaatgc cgtcatcccg cccaccgcat acttcgccgc
    36781 aaggttgagg aactcacgtt tcgaaatttt cccgtgaacg taatagtcgt agtaatccaa
    36841 caactcttgt gggaaatctt tggcggttaa acgcggcatc gttgcattcc tcaactggtg
    36901 tttttttaag caaagcataa gcgcgtgttt ttgcccagtt tttcgtcact ctgtgagcca
    36961 gactacggga tacgcgctgg cgaatcgcta aactagaaac attgtttcga aattgaacgg
    37021 tggaaaggag aggtcatggt ctggttagcg aatcccgaac gttacgggca gatgcagtac
    37081 cgctattgcg gaaaaagtgg tttacgcctg cccgcgttat cgctcggttt atggcacaat
    37141 ttcggtcacg ttaacgcgct ggaatcacag cgtgcaatcc tgcgtaaagc gtttgattta
    37201 ggcattacgc actttgattt agccaacaat tacgggccgc ctccaggaag cgcagaagag
    37261 aactttggtc gcctgctgcg cgaggatttt gctgcttatc gcgatgaact gattatctct
    37321 accaaggctg gctacgatat gtggcccggc ccgtacggct ctggcggttc acgtaaatac
    37381 ctgctcgcca gcctcgacca aagcctaaag cgtatggggc ttgagtatgt cgatatcttt
    37441 tactctcatc gcgtcgatga aaatacgccg atggaagaaa ccgcctctgc gctggctcat
    37501 gcggtacaaa gcggtaaggc gctgtatgtc gggatctcct cttactcgcc agagcggacg
    37561 caaaaaatgg tcgagttgct gcacgagtgg aaaattccgc ttttaattca tcaaccttcg
    37621 tacaatttac tgaaccgctg ggtggataaa agcggcctgc tggataccct gcaaaataac
    37681 ggcgtgggct gtattgcctt tactcctctg gctcagggat tgctgaccgg aaaatatctc
    37741 aacggcattc cgcaagattc acggatgcat cgtgaaggga ataaaattcg tggtctgacg
    37801 ccgaaaatgc ttaccgaagc caacctcaac agcctgcgct tattgaatga aatggcacag
    37861 cagcgtggac aatcaatggc gcaaatggcg ttaagctggt tgctgaaaga tgatcgcgtg
    37921 acgtcggtat tgattggtgc cagccgcgcg gagcaactag aggagaacgt gcaggcgctg
    37981 aataatctga catttagcac cgaggagctg gcgcagattg atcagcatat cgccgatggc
    38041 gaactgaatc tgtggcaggc gtcgtccgat aaatgacctg ttaataacgg gcgcgcgaac
    38101 cgcgcccgaa tcagatcagt gattatgacg agtcagtcgg tcaagatagc ccatcacaaa
    38161 tgcagaaagc acaaacgtca gatggataat gacgtaccac atcagtttgt tatccgggac
    38221 atttttcgca tccataaaga cgcgcagtaa gtgaatggaa gaaattgcca caatcgacgc
    38281 tgctactttg tttttcagcg acgttgcgtc cattttcccc agccagttca gcttctcttt
    38341 gttctcggag atatccagct gtgagacgaa attctcataa ccggaaaaca tcaccatcac
    38401 cagcaaaccg ccaaccagcg tcatatccac cagcgacagc aacacgagga tcaaatctga
    38461 ttccgccatc gagaagatat tcggcagtac gtgaataatc tcctggaaga acttcagcgc
    38521 cagggcaact aacgccagcg aaaggccaaa gtacacgggg gcaagcagcc agcgagaagc
    38581 atacattgca ttttcaagaa aacgttccat acattcctgt cgtgtagtta aaacgggcaa
    38641 cagtatatcg caattgagta aacagcacgc aacagcaaat caacatatac gttaaacctt
    38701 tgtcgcataa ttctgaggtt tagggatctt cctggttccc ttatcaacgc atcgagagcg
    38761 tctaccctta aaggacgttg aacctgaagg gagaaaaacg atgtctcatt taaaagaccc
    38821 gaccacgcag tattacactg gtgaatatcc caaacagaaa caaccgacgc caggcatcca
    38881 ggcgaagatg acaccgatac cggattgcgg cgagaaaacc tatgttggta gcgctcgcct
    38941 gaaagatcgt aaagcactgg tgacaggggg cgattccgga ataggtcgcg ctgccgccat
    39001 cgcttacgcg cgagaagggg ctgacgtggc gatcagttat cttcccgtgg aagaagaaga
    39061 cgctcaggat gtgaaaaaga tcattgaaga atgcggacgc aaagccgttc tgctgccagg
    39121 cgatttaagc gatgagaaat tcgcccgttc gctggttcac gaagcgcaca aggcgttagg
    39181 cgggctggat attatggcgc tggtcgccgg gaaacaggtt gccattccgg atattgcaga
    39241 cctcaccagc gaacagtttc aaaagacctt tgccattaac gttttcgcgc tgttctggct
    39301 aacccaggaa gcgatccccc tgctaccgaa aggtgcaagt atcatcacca cttcgtcaat
    39361 ccaggcatac cagccaagtc cgcatttact ggactatgcg gctacgaagg cggcgattct
    39421 gaactacagc cgtggcctgg caaaacaggt cgcggagaaa ggtattcggg tgaatattgt
    39481 cgcgccaggc cctatctgga cggcactgca aatttccggc ggacaaacgc aggataagat
    39541 cccgcagttt ggtcagaaaa cgccgatgaa gcgtgcgggg caaccggcgg aactggcccc
    39601 tgtatatgtt tatctggcaa gtcaggagtc gagctacgtc accgcagaag tgcacggcgt
    39661 gtgcggcggc gagcatttag gttaaaaaaa tgcccggttg tgaaaagcaa ccgggcatca
    39721 ttgtgaattc actcttacct gatgtggcga aaacgcctta tccgcctaca aacgcatgaa
    39781 gattcaacat gttgcaatag attttgtagg cctgataagc gtagcgcatc aggcattgag
    39841 cacctaatgc cggatgcgtc gtgaacgcct tatccggcct acaaacgcac gaagattcaa
    39901 catgttgcaa tagattttgt aggcctgatg agcgtagcgt atcaggcaat tctactttac
    39961 ttcgctttgg cggtttcttc gccgaccaga cctatcttca ggtaacccgc ctgatgcagc
    40021 gtatccatta ccttcatcaa cgtctcgtaa tcgacggttt tgtccgctcg gaagaagatg
    40081 gtggtgtctt tcttgccttc ggttaacgca ttcaacgccg taatcattgt ttcatcggtg
    40141 accggatcgt taccgataaa catcgagttg tctgccttca ccgacagata aaccggtttt
    40201 tccgggcgcg gctgcggcgt gctggtagaa gcaggcaagt tcaccttcac atctaccgtc
    40261 gctaacggtg ccgccaccat aaagataatc agcagaacca acatcacgtc gataaacggc
    40321 gtcacgttga tatcatgcat ttcgccgtta tcgtcgaggt tttcgttaag atgcattgcc
    40381 atcggacatt atcctgcgcg taatttttgt gcgacacgaa ccggatgcgc agcggcgctg
    40441 gcttccagat ccaggtcacg gctttgcagc aacaatacct gcgctgcaac atcacccagc
    40501 atcgctttaa agccgccaat ctggcgtgca aatacgttat agataacgac cgcaggaatc
    40561 gctgccacga ggccgattgc cgttgctaac agagcttctg cgatacccgg cgcaacgact
    40621 gccaggttag tggtctgcgt ttgcgcgata ccaataaagc tgttcatgat gccccagacc
    40681 gtaccaaaca gaccaacgaa cggagaaatc gcgccgatgg ttgccagata gccgttaccg
    40741 cggcccattt gacgacccac tgcggcgacc cgacgctcca gacggaagct ggtacgttct
    40801 ttaatacctt cgttatcgtc gctgccttct gacagttcca gctcattctg cgcttcattg
    40861 agcaaatgca ggcttaagct tttgctacca aaatcagcgg cgatatcgtt ggcctggttt
    40921 aaggaacgtg cttcagccag cagttgctgc tcgcgcttaa ggcgacgctt ctgattgaag
    40981 aactctacgc tcttactgaa gaagattgcc caggtgacta cggaggccaa aataagccca
    41041 atcatcacgc acttaacgac aatatcggcg tgctgataca taccccagac ggaaaggtcc
    41101 gtctgcatta aattattacc cactgtgtat ctccaggacg caagtcacaa aatctgcgca
    41161 taataatatc aaaacgacgt cgaattgata gtcgttctca ttactatttg catgctgccg
    41221 tacctttgct ttcttttcct tgcgtttacg cagtaaaaaa gtcaccagca cgccatttgc
    41281 gaaaattttc tgctttatgc caattcttca ggatgcgccc gcgaatattc atgctagttt
    41341 agacatccag acgtataaaa acaggaatcc cgacatggcg gacaaaaagc ttgatactca
    41401 actggtgaat gcaggacgca gcaaaaaata cactcttggc gcggtaaata gcgtgattca
    41461 gcgcgcttct tcgctggtat ttgagagtat ggaagccaaa aagcacgcga cgcgcaatcg
    41521 cgccaatggc gagttgttct atggacggcg cggaacgtta acccattttt ccttacaaca
    41581 agcgatgtgt gaactggaag gtggcgcagg ctgcgcgcta tttccctgcg gggcggcagc
    41641 ggttgctaat tcgattcttg cttttgtcga acagggcgat catgtgttga tgaccaacac
    41701 cgcctatgaa ccgagtcagg atttctgtag caaaatcctc agcaaactgg gcgtaacgac
    41761 atcgtggttt gatccgctga ttggtgccga tatcgttaag catctgcagc caaacactaa
    41821 aatcgtgttt ctggaatcgc cgggttccat cactatggaa gtccacgacg ttccggcgat
    41881 tgttgccgcc gtgcgcagtg tggtgccgga tgccatcatt atgatcgaca acacctgggc
    41941 agccggtgtg ctgtttaagg cgctggattt tggcatcgat gtttctattc aggccgccac
    42001 caaatatctg gttgggcatt cagatgcgat gattggcact gccgtgtgca atgcccgttg
    42061 ctgggagcag ctacgggaaa atgcctatct gatgggccag atggtcgatg ccgataccgc
    42121 ctatataacc agccgtggcc tgcgcacatt aggtgtgcgt ttgcgtcaac atcatgaaag
    42181 cagtctgaaa gtggccgaat ggctggcaga acatccgcaa gttgtgcgag ttaaccaccc
    42241 tgccctgcct ggcagtaaag gacacgaatt ctggaaacga gactttacag gcagcagcgg
    42301 gctattttcc tttgtgctta agaaaaaact caatgatgaa gagctggcga actatctgga
    42361 taacttcagt ttattcagca tggcctactc gtggggcggg tatgaatcgt tgatcctggc
    42421 aaatcaacca gaacatatcg ccgccattcg cccacaaggc gagatcgatt ttagcgggac
    42481 cttgattcgc ctgcatattg gtctggaaga tgtcgacgat ctgattgccg atctggacgc
    42541 cggttttgca cggattgtat aacattgcca cttttggaca attttgcaga cattttattg
    42601 tgaaaagtct taaattgttg cgtccgggat caaggcgtcc cggacgattc aggagtacaa
    42661 taggcagata aaggcttaaa cgctgttcca caggaaagtc catggctgtt attcaagata
    42721 tcatcgctgc gctctggcaa cacgactttg ccgcgctggc ggatcctcat attgttagcg
    42781 ttgtttactt tgtcatgttt gccacgctgt ttttagaaaa cggcctgctg cccgcctcat
    42841 ttttgccagg cgacagcttg ttgatactgg caggcgcatt gattgcccaa ggggttatgg
    42901 attttctgcc tacgattgcg attctgaccg ccgcagcaag tctgggctgc tggctaagtt
    42961 atattcaggg gcgctggtta gggaatacca aaacggtgaa aggctggctg gcacagcttc
    43021 ctgctaaata tcaccagcgc gccacctgca tgtttgaccg ccacggtctg ctggcgctgc
    43081 tggctggacg ttttcttgca tttgtccgta cgctgctgcc aaccatggcg ggaatttccg
    43141 gtctgccaaa ccgccgcttc cagtttttca actggttaag tggattgctg tgggtcagcg
    43201 tggtaaccag ttttggctat gccttaagta tgattccgtt cgttaaacgc catgaagatc
    43261 aggtaatgac gttcctgatg atcctgccaa ttgccttgtt aaccgctggc ttgttaggca
    43321 cgctgtttgt ggtgattaaa aaaaaatact gtaacgcctg acgattttcc ccgttcccgg
    43381 ctgctgtacc gggaacgtat ttaattcccc tgcattgccc gcattcttgc cgcatcttcc
    43441 cccggcgtca caccgaagta acgtttaaac tcacggctaa attgcgatgc gctttcatag
    43501 ccgacgcgca tcgctgctgc gctggccttc atgccgtcat ggatgatcat catccgcgcc
    43561 ttatgcagac ggtaattctt caaatactgc aacggcgagg tgctggtgac agacttaaaa
    43621 ttatggtgga acgccgatac gctcatgttg gcttctgccg ccagttgctc gacgctcagg
    43681 ttttcggtgt atttattctc aattcgtttc agcacgcggc taatcagact gaagtgagtc
    43741 tggcgactga ccagcgccag taacgcgccg ccgcaaggtc cggtcagcac gtagtacaga
    43801 atttcgcgga tgatctgttt gccgagaata cgcgcatcca gtggtcgctc catcacgtcg
    43861 agtaaccgct ccgccgcgaa taaaatctct tctgataacg tggcggagtt aatcccgctg
    43921 gctgccatcg acggctggaa atgctcatct tcgccaatgt ccatcaacag ttcctgtaac
    43981 tgcaaaatat cgacattgag acgcaaccct gccagcggca cctctgacgt ggcataggtt
    44041 tcgcactcaa acggcaacgg caccgtcagc agcaggtatt cattggcatc ataacgaaac
    44101 acgcg
//
LOCUS       DAETPZ010000025        43604 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.025, whole genome shotgun sequence.
ACCESSION   DAETPZ010000025 DAETPZ010000000
VERSION     DAETPZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.025"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..413
                     /locus_tag="K0W59_001571"
     CDS             <1..413
                     /locus_tag="K0W59_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBI3022662.1"
                     /translation="MYIGAPLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKL
                     FWRPKGLAYPLGEAPAKLRCTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFD
                     KYQFSLPKGAKNSSVTYRTINDYGAETPQLNCKS"
     gene            438..3037
                     /locus_tag="K0W59_001572"
                     /pseudo
     CDS             438..3037
                     /locus_tag="K0W59_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3028..4098
                     /locus_tag="K0W59_001573"
                     /pseudo
     CDS             3028..4098
                     /locus_tag="K0W59_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4110..4652
                     /locus_tag="K0W59_001574"
     CDS             4110..4652
                     /locus_tag="K0W59_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3022663.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4660..5175
                     /locus_tag="K0W59_001575"
     CDS             4660..5175
                     /locus_tag="K0W59_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBI3022664.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5141..5878
                     /locus_tag="K0W59_001576"
     CDS             5141..5878
                     /locus_tag="K0W59_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBI3022665.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRHEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5989..6999
                     /gene="pyrD"
                     /locus_tag="K0W59_001577"
     CDS             5989..6999
                     /gene="pyrD"
                     /locus_tag="K0W59_001577"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HBI3022666.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7173..7715
                     /gene="zapC"
                     /locus_tag="K0W59_001578"
     CDS             7173..7715
                     /gene="zapC"
                     /locus_tag="K0W59_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HBI3022667.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7712..8821)
                     /gene="ycbX"
                     /locus_tag="K0W59_001579"
     CDS             complement(7712..8821)
                     /gene="ycbX"
                     /locus_tag="K0W59_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HBI3022668.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            9065..11173
                     /gene="rlmKL"
                     /locus_tag="K0W59_001580"
     CDS             9065..11173
                     /gene="rlmKL"
                     /locus_tag="K0W59_001580"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HBI3022669.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPSATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11185..13092
                     /locus_tag="K0W59_001581"
                     /pseudo
     CDS             11185..13092
                     /locus_tag="K0W59_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            13222..14475
                     /gene="pqiA"
                     /locus_tag="K0W59_001582"
     CDS             13222..14475
                     /gene="pqiA"
                     /locus_tag="K0W59_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HBI3022670.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQLLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14480..16120
                     /gene="pqiB"
                     /locus_tag="K0W59_001583"
     CDS             14480..16120
                     /gene="pqiB"
                     /locus_tag="K0W59_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HBI3022671.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16117..16680
                     /gene="pqiC"
                     /locus_tag="K0W59_001584"
     CDS             16117..16680
                     /gene="pqiC"
                     /locus_tag="K0W59_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HBI3022672.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16936..17103
                     /gene="rmf"
                     /locus_tag="K0W59_001585"
     CDS             16936..17103
                     /gene="rmf"
                     /locus_tag="K0W59_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HBI3022673.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17173..17691)
                     /gene="fabA"
                     /locus_tag="K0W59_001586"
     CDS             complement(17173..17691)
                     /gene="fabA"
                     /locus_tag="K0W59_001586"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HBI3022674.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17760..19520)
                     /locus_tag="K0W59_001587"
     CDS             complement(17760..19520)
                     /locus_tag="K0W59_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HBI3022675.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPHWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19705..20157
                     /gene="matP"
                     /locus_tag="K0W59_001588"
     CDS             19705..20157
                     /gene="matP"
                     /locus_tag="K0W59_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HBI3022676.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20233..21279)
                     /gene="ompA"
                     /locus_tag="K0W59_001589"
     CDS             complement(20233..21279)
                     /gene="ompA"
                     /locus_tag="K0W59_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HBI3022677.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21635..22144)
                     /gene="sulA"
                     /locus_tag="K0W59_001590"
     CDS             complement(21635..22144)
                     /gene="sulA"
                     /locus_tag="K0W59_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HBI3022678.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22363..22992
                     /gene="sxy"
                     /locus_tag="K0W59_001591"
     CDS             22363..22992
                     /gene="sxy"
                     /locus_tag="K0W59_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HBI3022679.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(22955..25117)
                     /gene="yccS"
                     /locus_tag="K0W59_001592"
     CDS             complement(22955..25117)
                     /gene="yccS"
                     /locus_tag="K0W59_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HBI3022680.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQAPQETPVSA"
     gene            complement(25127..25573)
                     /locus_tag="K0W59_001593"
     CDS             complement(25127..25573)
                     /locus_tag="K0W59_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBI3022681.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            25696..27750
                     /gene="helD"
                     /locus_tag="K0W59_001594"
     CDS             25696..27750
                     /gene="helD"
                     /locus_tag="K0W59_001594"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HBI3022682.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(27782..28240)
                     /gene="mgsA"
                     /locus_tag="K0W59_001595"
     CDS             complement(27782..28240)
                     /gene="mgsA"
                     /locus_tag="K0W59_001595"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBI3022683.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28336..28998)
                     /locus_tag="K0W59_001596"
     CDS             complement(28336..28998)
                     /locus_tag="K0W59_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HBI3022684.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29171..29584
                     /locus_tag="K0W59_001597"
     CDS             29171..29584
                     /locus_tag="K0W59_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBI3022685.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29629..29946)
                     /gene="hspQ"
                     /locus_tag="K0W59_001598"
     CDS             complement(29629..29946)
                     /gene="hspQ"
                     /locus_tag="K0W59_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HBI3022686.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30004..31194)
                     /gene="rlmI"
                     /locus_tag="K0W59_001599"
     CDS             complement(30004..31194)
                     /gene="rlmI"
                     /locus_tag="K0W59_001599"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HBI3022687.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31289..31567
                     /gene="yccX"
                     /locus_tag="K0W59_001600"
     CDS             31289..31567
                     /gene="yccX"
                     /locus_tag="K0W59_001600"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBI3022688.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31564..31893)
                     /gene="tusE"
                     /locus_tag="K0W59_001601"
     CDS             complement(31564..31893)
                     /gene="tusE"
                     /locus_tag="K0W59_001601"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HBI3022689.1"
                     /translation="MLIFEGKEIEMDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(31984..32643)
                     /gene="yccA"
                     /locus_tag="K0W59_001602"
     CDS             complement(31984..32643)
                     /gene="yccA"
                     /locus_tag="K0W59_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HBI3022690.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(32850..32937)
                     /locus_tag="K0W59_001603"
     tRNA            complement(32850..32937)
                     /locus_tag="K0W59_001603"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32901..32903),aa:Ser,seq:tga)
     gene            33363..34481
                     /gene="hyaA"
                     /locus_tag="K0W59_001604"
     CDS             33363..34481
                     /gene="hyaA"
                     /locus_tag="K0W59_001604"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HBI3022691.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLYYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPSCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            34478..36253
                     /gene="hyaB"
                     /locus_tag="K0W59_001605"
                     /pseudo
     CDS             34478..36253
                     /gene="hyaB"
                     /locus_tag="K0W59_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
     gene            36272..36979
                     /gene="hyaC"
                     /locus_tag="K0W59_001606"
     CDS             36272..36979
                     /gene="hyaC"
                     /locus_tag="K0W59_001606"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HBI3022692.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            36976..37563
                     /locus_tag="K0W59_001607"
     CDS             36976..37563
                     /locus_tag="K0W59_001607"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HBI3022693.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGRLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            37560..37958
                     /gene="hyaE"
                     /locus_tag="K0W59_001608"
     CDS             37560..37958
                     /gene="hyaE"
                     /locus_tag="K0W59_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HBI3022694.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            37955..38812
                     /locus_tag="K0W59_001609"
     CDS             37955..38812
                     /locus_tag="K0W59_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HBI3022695.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINSTGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            38946..40490
                     /gene="appC"
                     /locus_tag="K0W59_001610"
     CDS             38946..40490
                     /gene="appC"
                     /locus_tag="K0W59_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HBI3022696.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            40502..41638
                     /gene="appB"
                     /locus_tag="K0W59_001611"
     CDS             40502..41638
                     /gene="appB"
                     /locus_tag="K0W59_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBI3022697.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            41650..41742
                     /gene="cbdX"
                     /locus_tag="K0W59_001612"
     CDS             41650..41742
                     /gene="cbdX"
                     /locus_tag="K0W59_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HBI3022698.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDLRLKS"
     gene            41822..43120
                     /gene="appA"
                     /locus_tag="K0W59_001613"
     CDS             41822..43120
                     /gene="appA"
                     /locus_tag="K0W59_001613"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HBI3022699.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(43235..43465)
                     /locus_tag="K0W59_001614"
                     /pseudo
     CDS             complement(43235..43465)
                     /locus_tag="K0W59_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine kinase"
BASE COUNT        10847 a        10627 c        11593 g        10537 t
ORIGIN      
        1 ttatgtacat tggtgcgccg ttggcgaaag acagagaaac ccttttcttc actagcgtac
       61 gggcagtccc ttcaacaacg aagcgggaag agggaaatac cctgaagatt gccacacaaa
      121 gcgtcatcaa acttttctgg cgaccaaaag gtttagcgta tcccttaggc gaggctccgg
      181 cgaaactgcg ttgcacttcg tcagctgaca tggttacggt cagtaaccca acaccttatt
      241 tcattaccct gacagacctg aaaataggtg gaaaagtagt taaaaatcaa atgatttccc
      301 cctttgataa ataccaattc tctctgccaa agggggccaa aaatagcagc gtaacgtatc
      361 gaaccatcaa tgactacggg gcggaaacgc cgcaactcaa ctgtaaatcg taagccgtct
      421 tcagttaaga gagcgagatg tatagaactc atcgacaaca cagcctgtta agctctggtg
      481 gagtgccatc gtttattggt gggctggtgg tgtttgtgtc ggcagcgttc aatgcacaag
      541 ctgaaacctg gttcgatcct gcttttttca aagatgatcc ctcaatggtg gccgatttgt
      601 ctcgtttcga aaaaggacaa aaaataacgc caggggttta tcgtgtcgat attgttctga
      661 atcagacaat tgtagatacg cgcaacgtca attttgttga gataacgcca gagaagggga
      721 ttgccgcctg tttgacgact gaaagcctgg atgcaatggg tgttaatact gatgcgtttc
      781 cggcttttaa acaactggac aaacaagcgt gtgtgccatt ggcggagatt attccggatg
      841 ccagcgtaac gtttaatgtg aataaactcc gtctggaaat ttcagtaccg caaatcgcca
      901 tcaaaagtaa tgcccgtggt tatgtccccc ctgaacgttg ggatgaaggg atcaacgcgc
      961 tattactggg atattcgttt agcggggcta acagtattca tagcagcgca gacagtgatt
     1021 ctggcgacag ctattttctg aatttaaaca gtggcgttaa tttaggccca tggagattgc
     1081 gcaacaattc aacatggagt cgcagtagtg gccaaaccgc agaatggaag aatctcagca
     1141 gctatttgca gcgggcggtt attccactaa aaggcgaact gaccgtaggt gatgattata
     1201 ctgcaggcga ttttttcgat agtgtcagct ttcgtggtgt gcagctggcg tcagatgaca
     1261 acatgctgcc agacagcctg aaagggtttg cgcctgtggt gcgtggtatc gccaaaagca
     1321 atgcccagat aacgattaag caaaatggtt acaccattta ccaaacttat gtatcgcctg
     1381 gtgcttttga aattagtgat ctctattcca cgtcgtcgag cggtgatttg ttagttgaaa
     1441 tcaaagaagc ggacggcagc gtcaatagct acagcgtacc gttttccagc gtgccattac
     1501 tccagcgtca ggggcgaatc aaatacgcgg tgacactggc gaaatacaga accaatagta
     1561 atgaacagca ggagagcaaa tttgcccagg ccacgttgca gtggggcgga ccgtggggaa
     1621 cgacatggta tggtggtgga caatatgctg aatattaccg tgccgccatg tttggtctgg
     1681 gatttaacct tggcgatttc ggagcaattt cgttcgatgc gacccaggcg aagagtacgc
     1741 tggcagagca aagcgaacat aaaggtcagt catatcgttt tctgtatgcc aaaacgctca
     1801 accaattggg cactaatttt caattgatgg gctatcgcta ttcgacgtcg ggtttctaca
     1861 ccctttccga caccatgtat aaacatatgg atggctacga atttaatgac ggtgatgatg
     1921 aagatacgcc gatgtggtcg cgttattaca atttgtttta caccaaacgt ggcaaactgc
     1981 aggtcaatat ctcccagcaa ttaggcgagt acggttcgtt ttatttaagt ggtagccagc
     2041 aaacttgctg gcataccgat caacaggatc ggctattaca gtttggctac aacacgcaaa
     2101 ttaaagatct ctcgctgggg gtttcctgga actacagtaa gtcccgtggt caacctgatg
     2161 ctgatcaggt gtttgcacta aatttttccc tgccgctcaa tctgttgctc cccagaagta
     2221 atgatagcta taccaggaaa aaaattacgc ctggatgacc tctaacacca gtatcgataa
     2281 cgaagggcac attacacaaa acctgggttt aacggagaca ctactcgatg acggtaacct
     2341 gagctacagc gtgcaacagg gatataacag cgaggggaaa acggctaatg gtagcgccag
     2401 catggactac aaaggggcgt ttgcagatgc ccgagtgggc tacaactaca gcgataacgg
     2461 cagtcaacaa caactgaact acgctctttc aggcagttta gttgcccatt cacagggcat
     2521 taccctgggg caatcgctgg gggaaactaa cgtgctgatt gcagcaccag gcgcagagaa
     2581 tactcgtgtg gcgaacagca ccgggctgaa aactgactgg cgcggatata ccgttgtccc
     2641 ctatgccact tcttatcggg aaaatcgaat cgcacttgat gcggcatcgt taaaacgtaa
     2701 cgtggatctt gaaaatgcag tagtcaacga ggttcccacc aaaggggcgt tggttctggc
     2761 ggagttcaat gcccatgcgg gagcaagggt attaatgaaa acatcaaagc agggtatacc
     2821 gctgcgtttt ggcgcgatag cgacgctgga cggcatacag actaatagcg gcattattga
     2881 tgatgatggc tcgctctata tgtccggttt gcccgctcag ggagcgataa ctgtacgctg
     2941 gggcgaagct cccgatcaaa tttgtcatat cagttaccag cttaccgaac aacaaattaa
     3001 ctctgcgatt acgcgaatgg atgccatatg cagataatct ttggagaaaa atgcgtgtta
     3061 ttactacgac tattttttgc cgccgtctta atgctatggt gcgctcaaac tgctgcttat
     3121 atcgggcagt gtcataccac tcaggggaat ccgtatattg gcgtcaattt tggcgttaaa
     3181 actctggatg aagaagaaaa tacggctgga gtagttaaag acaaatttta tcagtggaac
     3241 gaatcgagtg attattatgt ttcctgtgat tgcgataaag acaatgtcag aagtggccga
     3301 tgggcattcg ccgcggattc accgttagtc tatttaggcg acaactggta caaaattaat
     3361 gactatcttg ccgccaaagt tttattgcag gttaaaggca gttctcctac tgcggttcct
     3421 ttcgaaaacg tgggcacagg ggcagataca cgatggcata tttgcgatcc cggcggtcaa
     3481 cgtttaggtg gctagggggc tagcggtaat agcggtagct tttccctgaa aatattgcag
     3541 ccgttcgttg gttcggtcgt cattcctcct atggcgctgg cgcgattatt tgaatgctac
     3601 aacatacccg caggtgattc ctgcacgact actggtacac cggttttagt gtattacctg
     3661 tctggtacga tcaattcact tggctcatgt tccgtcaatg ccggagaaac aatcgaggtt
     3721 gatctgggcg acgtatttgc ggctaacttt cgtgttgtag ggcataagcc tcttggggcc
     3781 agaacggcag aacttgcaat tccggtcagg tgtaacacgg gaaacgcagg gttagttaac
     3841 gtcaatctga gtctgacggc aacctcagac cccagctatc cccaggcgat taagacgtca
     3901 cgtcctggcg tgggcgtggt ggtgaccgat agccagaaca acattatttc ccctgctggt
     3961 gggacactac cgctctctat tcctgatgat gcagacagta tcgcgcgaat gaatgtctat
     4021 ccagtcagca cgacaggcgt accaccagaa accgggcgat ttgaagccac ggcaacggtg
     4081 agaataaatt ttgattaacg cgtgaacgta tgaagaaaaa aacgatatat cagtgcgtta
     4141 ttttgttctt tagccttctt aacatccatg tcgggatggc tgggcctgaa caagtcagta
     4201 tgcatattta tgggaatgtg gtcgatcagg gctgtgatgt cgccaccaaa agtgcattac
     4261 aaaatattca tattggtgat tttaatatca gtgattttca ggccgcgaat accgtaagca
     4321 ctgctgctga tttgaatatt gatatcaccg gttgtgccgc tggtattact ggcgcggacg
     4381 tcctttttag cggcgaggct gacacccttg cgccgacact gctcaaacta actgacacag
     4441 gcggaagcgg tggtatggca acggggattg ccgtgcaaat tcttgatgcg caaagtcagc
     4501 aagaaatccc gctcaatcag gtccagcctc ttacgccctt aaaagccggg gataacacac
     4561 tcaaatatca acttcgttat aagtccacaa aggcgggagc aacgggcggt aatgcgacgg
     4621 cggttctcta ttttgatctg gtttaccagt gaaggagtga tgttgaaacg gataatctgg
     4681 attctgttct tactgggatt aacggggggc tgtgagctat ttgcccatga tggcacggtc
     4741 aacattagcg gatcgtttcg ccgtaataca tgcgtgctgg cacaggatag caagcaaatc
     4801 aatgtgcagt taggcgatgt ctcgctgaca cgtttttttc atgggaatta tggcccggag
     4861 aaatctttca tcattaattt gcaggattgc ggaacagatg tcagcacggt tgatgtcacc
     4921 ttttcaggaa cccccgacgg agtgcagagc gaaatgctgt ccattgaaag tggtacggat
     4981 gcagccagtg ggctggcaat tgcgattctg gatgatgcga agatattgat tccgcttaac
     5041 caggcgagta aagactacag cctacatagc ggtaaggtgc cattaacttt ttatgcgcaa
     5101 ttgcgacctg tcaatagcga tgtgcagtcg ggtaaagtga atgccagcgc gacatttgta
     5161 cttcattatg actaatacgt ggaacagatt agcgctcttg attttcgccg ttttatcgct
     5221 gctggtggcg ggtgaattgc aggcaggagt ggtggtcggc ggaacgcgat ttatctttcc
     5281 ggcagacaga gaatcgatat ctattttact gactaatacc agtcaggaat cttggcttat
     5341 taacagcaaa atcaatcgcc caacgcgttg ggccgggggg gaagcgtcga cagtgccagc
     5401 actattactg gccgctccgc cacttattct cctgaagccc ggtactacag gcacgttgcg
     5461 cttgctgaga acggaaagcg acatcttgcc tgtggatcgc gaaacgctat ttgagttaag
     5521 cattgccagc gtgccatccg gcaaagttga aaatcagagc gtaaaagtgg cgatgcgctc
     5581 ggtatttaaa ctgttctggc gacacgaagg tttgccgggc gacccgctgg aagcttatca
     5641 acaattacgc tggacacgga attcgcaggg tgtacaactc actaacccaa cgccttacta
     5701 cattaacctg attcaggtga gcgtaaatgg taaagcgtta agtaatgcgg gagtcgtgcc
     5761 gcctaaaagc cagcgtcaga caaactggtg tcaggtaata gcaccgtgtc atgtcgcctg
     5821 gcggacgata aatgattacg gcgggttgag tgcaaagaag gagcaaaatc tgccctgaaa
     5881 caggttctga aaacgtttgc gttttttttg ccgcaggtca attccctttt ggtccggact
     5941 cgcacataat acgcccccgg tttgcacacc gggaatccag gagagttcat gtactacccc
     6001 ttcgttcgta aagccctttt ccagctcgat ccagagcgcg ctcatgagtt tacttttcag
     6061 caattacgcc gcattaccgg aacaccgttt gaagcactgg tgcggcagaa agtgcctgcg
     6121 aaacctgtta actgcatggg cctgacgttt aaaaatccgc ttggtctggc agccggtctt
     6181 gataaagacg gggagtgcat tgatgcgtta ggcgcgatgg gatttggatc gatcgagatc
     6241 ggtaccgtca cgccacgtcc acagcctggt aatgacaagc cgcgtctctt tcgtctggta
     6301 gatgccgaag gtttgatcaa ccgtatgggc tttaataatc ttggcgttga taacctcgta
     6361 gagaacgtaa aaaaggccca ttatgacggc gtcttgggta ttaatatcgg caaaaataaa
     6421 gatacgccag tggagcaggg caaagatgac tatctgattt gtatggaaaa aatctatgcc
     6481 tatgcgggat atatcgccat caatatttca tcgccgaata ccccaggatt acgcacgctg
     6541 caatatggtg aagcgctgga tgatctctta accgcgatta aaaataagca aaatgatttg
     6601 caagcgatgc accataaata tgtgccgatc gcagtgaaga tcgcgccgga tctttctgaa
     6661 gacgaattga tccaggttgc cgatagttta gttcgccata atattgatgg cgttattgca
     6721 accaatacca ctctcgatcg ttctctggtt cagggaatga aaaattgcga tcaaaccggt
     6781 ggcttaagtg gtcgtccgct tcagttaaaa agcacagaaa ttattcgccg cttgtcacta
     6841 gaattaaacg gtcgcttacc gatcatcggt gttggcggca tcgactcggt tatcgctgcg
     6901 cgtgaaaaga ttgctgcggg tgcctcactg gtgcaaattt attctggttt tatttttaaa
     6961 ggtccgccgc tgattaaaga aatcgttacc catatctaat tatttcttcg aattcctctt
     7021 tataagataa tcagggcttt attttcagcc ctggttgttt tatattcatc gctgttgctt
     7081 atttagacat tttgtacttt tattgttgag gttattaagc gaagcaacaa tggattgtgt
     7141 tgctgcggtt ttatagggta ggggagaggc agatgcgaat taaaccagac gataactggc
     7201 gttggtatta cgatgaagag cacgatcgaa tgatgctcga tttagccaat ggtatgctat
     7261 ttcgctcacg ttttgcgcgc aaaatgttga ctccagatgc cttttctccc gcaggctttt
     7321 gcgttgatga tgccgcgctc tatttctctt ttgaagaaaa gtgccgcgat tttaatttat
     7381 ctaaagacca aaaagccgaa ctggtgctga atgcactggt agcaattcgt tatctcaaac
     7441 cgcaaatgcc gaaaagctgg cattttgtct cccatggtga aatgtgggtg cccatgccgg
     7501 gcgatgcggc ctgcgtttgg ttgagcgata ctcacgagca ggtcaatttg ctggtcgttg
     7561 aatctggcga aaatgccgcg ctatgcctgc tggcacaacc ctgcgttgtt attgcgggtc
     7621 gcgcgatgca gttgggcgac gccattaaaa tcatgaacga caggctgaaa ccgcaggtga
     7681 atgttgacag cttcagcctc gaacaggcag tctaacgcgt cagcttaagg gcagtcttcg
     7741 gtacacagct acagcaaaga atggtgccat catcgcccat tgctgatttt ttcagcggcg
     7801 tgacttcgcc ttctaaaagc tgaacacggc aacttccaca aatgcccgcg cggcaagaat
     7861 aagggatacg aattccctga ttttctaatt gctccagcaa cacttgttgg ttatttccac
     7921 gaaatgcctg tccctgccag tcaatatcta catttgcgtc cggttgttgc gtgatgttgg
     7981 cggtatcatc agcggcagct gcgccgtaaa tttttgccgg agccgttgcc agaatttcca
     8041 cctcatcgcc aacgcgaatc acgccgctat tacgggcaat taaattctga ccaaaatcga
     8101 catcgccgtt atcctgggca gtgcggaaag attgcaatgt ttttaatggt tcgcctgccg
     8161 gatgtttttg ccctttttct gggctgacgg tggtgaaaat acagcggcta caaggtttaa
     8221 ccacatcaaa caccacatca ccaatgcgaa tcactttcca gctatcttct tcccaggctg
     8281 acgcgccgga aaccaccaga ttggggcgga attgctccat ttttacactg gccggacaac
     8341 gttgttggag atcacgtaac gaggcttcgt tagcaagaag gtaaggatag ccatcagcaa
     8401 atgacagagg tacagtgttg tggcgtttca cgcgccgggt catttgtggc cccacccagc
     8461 gtaattgcac ttcgcgggag aaaaatccac ttagccattt gttgatcgcg tctggcgcaa
     8521 ttcgcgcggt aaaatgtgtg ccccaaactt cggttggtgc gtcttgtgtg gcgaaatcag
     8581 caaaacgaac atatgcgcta ctgccatctg gtgcggtgag atgcaagcca tcatgcacag
     8641 gtgaaggagt aaaccgtacc atctggggaa actggcgagc ggtaataaac gtaccgtcag
     8701 gttccgtgat cataaagatg cgatcgaagg ccagaccact gacatcagcc agagcatgtg
     8761 taagaccaat gccgcgcatc gatttaacag gatgaataaa aagccgggtt aatgtcgcca
     8821 cggtgcggtc ctcaaatgaa aataagccct caactttatg acatgacgcg cttattagct
     8881 ataatgcgca acaattttct tagcgcctgt cccgttaggc tattttattt gtcagtttgg
     8941 ccctgggttg tgctcgaaat cctcacgtac ttgtgtacgc tccggtttct ccgcgctctc
     9001 catgtccaga ctgactgcaa caattacgcc tactgcggta ggctcttaga gtaaaagtga
     9061 cgatatgaat tctctgtttg ccagtacggc ccgtgggctg gaagagctgt taaaaactga
     9121 actggaaaat ctgggggccg ttgaatgcca ggtggttcag ggtggggtcc atttcaaggg
     9181 cgacacacgg cttgtttacc agagcctgat gtggagccgc ctggcctcgc gtattatgtt
     9241 gccgctgggc gagtgtaagg tttacagcga tttagacctc tatctcggtg ttcaggcgat
     9301 caactggaca gagatgttta atcctagcgc gaccttcgct gtccacttca gtggtttgaa
     9361 tgacaccatc cgcaacagtc agtacggcgc gatgaaagtg aaagacgcga tcgtcgatgc
     9421 tttcacgcgg aaaaatctgc cgcgtccaaa tgttgatcgc gatgcgccgg atatccgcgt
     9481 taacgtctgg ctgcataaag aaaccgccag tatcgctctt gatctgagtg gtgatggttt
     9541 acatctgcgt ggctatcgcg atcgtgctgg tattgcgccg atcaaagaaa ccctggcagc
     9601 cgctattgtg atgcgatccg gctggcagtc aggaacaccg ctgctcgatc cgatgtgtgg
     9661 ttccggtacg ttgctgattg aagcggcgat gctggcgacc gatcgcgcac caggcttgca
     9721 ccgtgggcgt tggggcttta gcggctgggc gcagcatgat gaagctatct ggcaggaagt
     9781 gaaagcggaa gcgcaaactc gcgcccgtaa aggcctggct gagtatagct ctcattttta
     9841 cggttcggac agcgacgcac gggtgattca acgtgcgcgc actaacgccc gtcttgcggg
     9901 gattggtgaa ctgatcacct ttgaggtgaa agatgtcgcg caactgacca atccgctgcc
     9961 gaaagggccg tacggtacag tgttgagcaa cccgccatac ggtgaacgtc tggacagcga
    10021 accggcgctg attgcgctgc atagtctgct gggccgaatc atgaaaaacc agttcggtgg
    10081 ctggaatctc tctttgttta gtgcctcgcc ggatctgcta agctgcctgc agctgcgtgc
    10141 agacaaacag tacaaggcga aaaacggccc gctggactgc gtacagaaaa attaccacgt
    10201 tgccgaaagc acaccagaca gtaaaccggc aatggtagcg gaagattacg ccaaccgcct
    10261 gcgtaagaac ctcaaaaaat tcgagaagtg ggcacgccag gaagggattg aatgttaccg
    10321 cctgtatgac gccgatctgc cggaatataa cgttgccgtt gaccgttatg ccgactgggt
    10381 ggtggtgcag gagtatgcgc cgccaaaaac tattgatgcg cacaaagcgc gtcagcgtct
    10441 gttcgatatt atcgctgcaa ccatttcggt actggggatt gcgccaaaca aactggtgct
    10501 gaaaacccgt gaacgccaga agggcaaaaa ccagtaccag aaactgggcg agaagggcga
    10561 gtttcttgaa gttaccgaat ataacgctca cttgtgggtg aacctgacgg attatctcga
    10621 tactggtctg ttcctcgatc accgtatcgc ccgccgtatg ctcggtcaga tgagcaaagg
    10681 caaagatttc ctcaacctgt tttcttatac cggcagcgcc accgtgcatg cgggattagg
    10741 cggtgcacgc agcaccacca ccgtggatat gtcgcgtact tatctggaat gggcagaacg
    10801 caacctgcgt ctgaatggcc tgactaggcg tgcgcatcgc ctgattcagg ccgattgcct
    10861 ggcgtggctg cgtgaggcaa atgaacagtt cgatctgatc tttatcgatc cgccaacctt
    10921 ctctaactca aaacgaatgg aagatgcgtt tgatgttcag cgcgatcatc tggcgctgat
    10981 gaaagatttg aaacgtctgc tgcgtgcagg tgggacgatc atgttctcga acaacaaacg
    11041 tggcttccgt atggatctcg acggcctggc aaaactggga ctgaaagcac aagaaattac
    11101 gcaaaaaacg ctctcccagg atttcgcccg taaccgccag atccacaact gctggctgat
    11161 taccgcagcc tgaaaggaat agtaatgtca ttaatcagta tgcatggcgc atggctgtcg
    11221 ttcagcgacg cgccgcttct cgataacgca gaactgcata tcgaagataa cgaacgtgtt
    11281 tgtctggtgg gccgcaacgg cgcaggaaaa tcgacgttaa tgaaaatcct caaccgtgaa
    11341 caagggctgg atgacggtcg cattatttat gagcaagatt tgattgtagc tcgtctgcaa
    11401 caggatccgc cgcgtaacgt tgagggtagc gtttatgatt tcgttgccga aggcattgaa
    11461 gaacaagcgg aatatctgaa acgctatcac gatatttcgt gcctggtgat gaacgacccg
    11521 agcgagaaaa atctcaacga actggcgaag gttcaggaac agctggatca ccacaacctg
    11581 tggcagctgg aaaaccgcat caacgaagtg ctggcgcaac tggggttaga tcctaacgtt
    11641 gcgctgtcgt cgctttccgg cggctggttg cgtaaagcgg cattaggacg cgcgctggtg
    11701 agcaatccgc gcgtattgct gctggacgaa ccgactaacc atctggatat cgaaaccatc
    11761 gactggctgg aggggttcct taaaaccttc aatgggacga ttattttcat ctcccacgac
    11821 cgttcgttta tccgcaatat ggcgacgcgc attgttgatc tcgatcgcgg caagctggtg
    11881 acctatccag ggaattacga ccagtacctg caggaaaaag aagaagccct gcgcgtggaa
    11941 gaactacaaa atgccgagtt cgatctcaaa ctggcgcagg aagaagtgtg gattcgctag
    12001 gggatcaaag cacgccgtac ccgtaatgaa ggccgcgtac gcgccctgaa agcgatgcgt
    12061 cgcgaacgtg gtgaacgtcg cgaagtgatg ggtaccgcaa agatgcaggt ggaagaggcc
    12121 agccgctccg gtaagatcgt tttcgaaatg gaagacgttt gctaccaggt taacggtaag
    12181 caactggtga aagatttttc tgcccaggtt ctacgtggcg acaaaattgc cctgattggt
    12241 ccgaatgggt gcggcaaaac cacgctgctg aaactgatgc tcggtcagct tcaagcgggc
    12301 agcgggcgta ttcacgttgg caccaaactg gaagtggctt atttcgatca gcaccgcgcg
    12361 gaactggatc ccgataaaac ggtgatggat aaccttgccg aaggtaagca agaggtgatg
    12421 gttaatggca agccacgcca cgtattgggc tatttgcagg actttctgtt ccatccgaaa
    12481 cgggcgatga cgccagtacg tgcgctttct ggcggtgagc ggaaccgctt gctgctggcg
    12541 cgtttgttcc tcaaaccaag caacttattg attcttgacg aaccgaccaa cgatcttgat
    12601 gtcgaaacgc tggaactgct ggaagaactg atcgacagct atcagggcac ggtattgctg
    12661 gttagccacg atcgtcagtt tgtcgataac accgttacag aatgctggat cttcgaaggc
    12721 ggcggtaaaa ttggtcgtta tgttggcggt tatcatgatg cccgtggtca gcaagaacaa
    12781 tatgtggcac tcaaacagcc tgcggtgaaa aaaagcgaag aagccgccgc gccaaaagca
    12841 gaaactgtaa aacgcagcag tagcaaacta agctataaat tgcagcgcga actggagcag
    12901 ctaccgcaat tgctcgaaga tctggaggcg aagctggaag ccctacagac gcaagtggcg
    12961 gatgcttcct tcttcagtca gccgcatgag cagacgcaaa aagtgcttgc tgatatggct
    13021 gctgcagagc aggagctgga gcaagccttt gaacgctggg agtatcttga agcgttaaaa
    13081 aatggtggct gatcgcaaaa tagtcgattt aaacagagac agctgacaac ggtaaaattg
    13141 cctgatgaac tgggtttatc aggcatgaaa accaacactt tgttagtcat ctgtaaaata
    13201 gcgcatcatt aaggagtacc aatgtgcgaa catcatcatg ccgcgaagca catcctgtgc
    13261 tcgcagtgtg acatgctggt ggcgttaccg cgccttgagc atggtcagaa agcggcatgt
    13321 ccccggtgtg gcacaacgtt aaccgtggcg tgggatgccc ctcggcagcg tcccaccgcc
    13381 tatgcgttgg ctgcactgtt catgctgttg ctgtccaact tgtttccttt tgtgaatatg
    13441 aacgttgcag gagttaccag tgaaattaca ttactggaaa ttcccggcgt gcttttttct
    13501 gaggactacg ccagcctcgg cacctttttc ctgttgtttg tgcaactggt tcccgcgttt
    13561 tgtctgataa ccattctgtt actggtgaat cgcgcggaat taccggtccg tttaaaagag
    13621 caactggcac gggtgctttt tcaactcaaa acctggggaa tggcggagat tttcctcgcg
    13681 ggtgtgctgg tcagtttcgt taaactgatg gcttacggca gcattggggt aggcagcagc
    13741 tttctcccct ggtgtttatt ttgtgtcctg caactgcgcg cttttcagtg cgttgatcgt
    13801 cgctggttat gggacgacat cgccccgatg ccagaactgc gccagctgct aaaaccaggc
    13861 gtcacgggga tacgtcaggg gctgcgttcg tgctcctgtt gtacggcaat ccttcctgct
    13921 gatgaacccg tgtgcccgcg ttgtagtacc aaagggtacg ttcggcgtag aaacagcctg
    13981 cagtggacac tcgcgctgct tgtaacgtcc atcatgctgt atcttccggc taatattttg
    14041 cccatcatgg tgacggattt attaggctcg aagatgccgt cgacgattct cgctggggtc
    14101 attctgttat ggagcgaagg ctcttatccc gtcgctgcgg tgatctttct ggccagtatt
    14161 atggtgccaa cgttaaagat gatcgccatc gcgtggctgt gttgggatgc caaagggcat
    14221 ggcaagcgcg acagtgaaag aatgcatttg atttatgaag ttgttgagtt tgtaggccgc
    14281 tggtcgatga ttgacgtttt cgttatcgcg gtgctctcgg cgctggtgcg tatgggaggt
    14341 ttaatgagta tttatccggc aatgggtgca ttaatgtttg ctttagtcgt cataatgaca
    14401 atgttttctg ctatgacgtt tgacccgcgt ttgtcgtggg atcgtcaacc tgaatcagag
    14461 catgaggagt cctgacagta tggaatctaa taatggggaa gccaaaatcc agaaagtgaa
    14521 gaactggtct cccgtgtgga tatttcctat cgtcacggcg ctcattgggg cctgggttct
    14581 tttttatcat tacagccatc agggaccgga agtgaccctg atcaccgcga atgcggaagg
    14641 aattgaaggt ggcaaaacca ccattaaaag ccgtagcgtt gacgtcggcg tggttgaaag
    14701 cgccacactg gctgatgatt tgacgcacgt tgaaatcaaa gcgcggctga attccggtat
    14761 ggaaaaattg ctgcataaag acaccgtctt ttgggtggtg aaaccgcaga ttggtcgcga
    14821 agggattagc ggcctgggaa cgctgctgtc tggagtttat atcgaactgc agccaggcgc
    14881 gaaaggcagc aaaatggata aatacgattt gctggactca ccaccgttgg ccccgcctga
    14941 tgcgaaaggt atccgtgtga ttctcgatag caaaaaagcc gggcagctct cgccaggaga
    15001 tccggtgctg ttccgtggct atcgggtagg ttcggttgaa accagcacct tcgatacgca
    15061 aaaacgcaat atcagttatc aactgttcat caatgcacct tatgaccgac tggtgaccag
    15121 caatgttcgc ttctggaaag atagtggcat tgcggttgat ctgacgtcag cggggatgcg
    15181 tgtggagatg ggctcattga caacgctgct tagtggcggt gttagctttg atgtgccgga
    15241 aggtctggat ttagggcagc cagtggcacc gaaaacagct ttcgttttgt atgatgatca
    15301 gaagagcatt caggattcgt tgtacaccga tcacattgat tatctgatgt tctttaaaga
    15361 ttcggtacgc ggtctgcaac cgggagctcc ggtagaattc cggggtattc gcctgggtac
    15421 cgtaagcaaa gtgccattct ttgcgccgaa tatgcgtcag acatttaacg atgattaccg
    15481 tattccggta ctgattcgta tcgagccaga gcggctgaaa atgcagcttg gcgaaaatgc
    15541 ggatgttgtt gagcaccttg gcgaattgtt gaaacgtggt ttacgcggat cgctgaaaac
    15601 cggaaacctg gtcactggtg cactgtatgt tgatctcgat ttctatccaa atacgcctgc
    15661 aataaccggt attcgtgaat ttaatggtta tcagattatc ccgactgtta gcggcggcct
    15721 ggcgcaaatc cagcaacgac tgatggaagc gttggataag atcaacaaac tgccattgaa
    15781 tccgatgatt gaacaggcaa ccagtacgct ttctgaaagt cagcgcacaa tgaaaaacct
    15841 gcaaacgacg ctggatagca tgaacaagat cctcgccagc cagtcgatgc agcagttgcc
    15901 gacggatatg cagtcaacgt tgcgtgaatt gaatcgcagc atgcagggct tccagcctgg
    15961 ctccgcagcc tacaacaaga tggtggcgga tatgcagcgc cttgatcagg tgttgcgaga
    16021 actgcaaccg gtgctgaaaa cgctcaatga gaagagtaac gcgctggtat ttgaagcgaa
    16081 ggacaaaaaa gatccagagc cgaagagggc gaaacaatga aaaagtggct agtgacgatt
    16141 gcagcactgt ggctggccgg atgcagctcc ggcgaaatta ataaaaacta ttaccagtta
    16201 cctgtggtgc agagcggtac acaaagtacc gccagccagg gcaatcgtct gttatgggta
    16261 gagcaggtca ctgttcctga ctatctggcg gggaatggtg tggtttatca aaccagtgat
    16321 gtgaagtatg tgattgccaa caacaacttg tgggccagcc cgttggatca acagttgcgc
    16381 aacaccctgg ttgccaacct gagtacgcaa ctgcccggct gggtggttgc ctcccagcct
    16441 ctgggaagcg cccaggacac gctcaatgtt accgtaacgg agtttaacgg tcgctatgat
    16501 ggcaaggtca ttgtcagtgg tgagtggctg ttgaaccacc agggacaact gatcaaacgt
    16561 ccgttccgtc tggaaggagt gcaaactcag gatggttacg atgagatggt taaagtgctg
    16621 gccggtgtct ggagtcagga agccgcttct attgcacaag agataaagcg tctaccttaa
    16681 ttataaagat ttgtaaatat aaccgtctcc ggtatgttgc ctgaggcggt ttttttgttt
    16741 ctaacgtgcg gaaaaatttg ttcctcttca cattttttgt acaaccgaca tgcccgtgta
    16801 gctcacaaat atgacagtgg cgtgaatttt gcgcattgac ggcagttatg attcgcggta
    16861 ttgcttaact gtgattgcac atttagtaat cactgttttc ttttccacca gaaaccagta
    16921 tgagggaaac gaggcatgaa gagacaaaaa cgagatcgcc tggaacgggc acatcaacgt
    16981 ggttatcagg ccggcatcgc cggacgctca aaagaaatgt gtccctatca gacgctgaat
    17041 caaaggtcac aatggctggg aggctggcga gaagccatgg cggacagggt agtaatggcc
    17101 tgattctgtc tctttaaaaa gaaacctccg cattgcggag gtttcgcctt ttgatactct
    17161 gtctgatgat aatcagaagg cagacgtatc ctggaacaga ccgactttca ggtcgctggc
    17221 ggtatagatc agacgacaat caaccagcac ttcgccatct gccagaccca taatcagacg
    17281 acgattaaca atgcgtttaa agtgaatacg gtaggtcact tttttcgctg tcggcagtac
    17341 ctgaccagtg aatttcactt cgccaacgcc cagcgcgcgg cctttacctt cgccgcccag
    17401 ccagccgagg tagaacccta ccagctgcca cattgcgtcc aggcccaggc atcccggcat
    17461 aaccggatcg ccaatgaagt ggcatccgaa gaaccacaga tccggattga tatccagttc
    17521 tgcttcaaca taccctttgt cgaagttacc acccgtttcg gtcattttga ccacacggtc
    17581 catcatcagc atgttcggtg ctggcaattg cgggccttta gcgccaaaca gttcaccgtg
    17641 accagaggca agaaggtctt cttttgtata ggattcgcgt ttatctacca tgttctctgt
    17701 aagccttatt ttattgaagc acgcaggata gctaacacgt gtacgctgaa caagtccgat
    17761 cagttcggaa taaaccagtt cagccaacgt aatggccatg gaaaacggtg acgtccttcc
    17821 tgttgcgatg cttgcgcgat acgttcctgg atggtttgca tcagcgtcgt ttggccttcg
    17881 ccatcccaca ccagatttaa taataacggc agtgcgtcag tcacatcgtc tactgcccag
    17941 atggtgaatt tgccttcttc taccgctttc accagttcac tgtgaagact taaatggcga
    18001 acgttagcag tggggataat gacaccttgt ttccccgtta actcacgttg ctggcaaata
    18061 gcaaagaagc cttcgatttt ctcatttaaa ccaccgaccg gctggacgcg gccgaactga
    18121 tcgactgaac ctgtgatagc gatactctga ttcactggca catcagcgag ggcgcttatc
    18181 agggcgcaga gttcagccat cgaggcacta tcgccatcca cttcactgta tgactgctca
    18241 aatgtcagcg atgctgagaa ggggatctgt tgctcaagct gtagttccga catcaggaac
    18301 gcctgcatga tcatcatccc tttcgcatgg atattgccgc caagctccgc tttgcgttcg
    18361 atgtcggtga attcaccatc gccaatatgc acaacgcagc taatgcgaga aggttcgcca
    18421 aaagcgcgtg gatgacccgg aaattcaatg accgaaaggg cgttaatttg cccgatgcgt
    18481 tcgccttcgg tttcaatcag gatttgctcc tgaaggatct catcctgcat acgttcagcg
    18541 aggaaacctt cgcgccattc acgctgctgc agcattaagt ttagctgctc gccggagaag
    18601 gtgtcgccat cacacaggga ggcgacctct ttacactggc ggaggatcca atgcgggcta
    18661 agcggaagtg tttcttgttc accggtgtag cgtgctgctt cgcggataag taccggccaa
    18721 gcatccgctc ccggggcagg taagtgatta tgtctggcgg taaatgtcac ccagcgacac
    18781 cactggctta ctgactccgc atcgacaatc tgcagagtat cttcaaattc gctataaata
    18841 gcctgctctg aaagctctgg ctccatctcc tggaaatcag ccaatgattc gcgttcgcct
    18901 accagaatga ctttcagctt caatggcatc gaaggcacag agacggggag agggcgcgac
    18961 tcatcaaacg caacccagtc aaaacgctcg cggttaacga tatttttcag ccgcatccac
    19021 agcagaggtt gcgccagcag tgtacgcaaa gagatgatga gaataccgcc atttgcctga
    19081 tgcaccagac caggctgcag ggtaatgtcg ccattaaact ggcgcaggca gccaaagagt
    19141 tgctccgctt ctacccagtc ggcagcgaca acttgcgtta aagtcgcaaa attatcatct
    19201 gcactcactg cgtgacgtaa gcggatggag tggccggaaa cctcatagtg atcgcccacc
    19261 agttggcctg catcgctttg tagcgtacgc gcggcatcgg caataagatt cagatactca
    19321 gactcttccg gggccttagc cagcataaaa gaggaggatg ctcgcgtatg cagcaactgc
    19381 tccagcgcaa attgcagtcg cggttgagta tcactgaata aaggatcgtt ttcgtcaatc
    19441 aaatgtggct gagcaaatat ttcctgatag ctatcggtat caggaaccag gtcacgccat
    19501 gcaagtttcg taatggtcaa agttgatgtt ttttagtctg ttgtcaaagc cgcgattata
    19561 ccgtaaccgg cactacagca cacgtagaaa gcaccgacaa tactcctggc tgggcgttaa
    19621 agctcacagg atggagattc ttttcttcac tggcctaaaa agctgatatt ctgtaaagag
    19681 ttacacggta acattgagat cgctatgaaa tatcaacaac ttgaaaatct tgaaagcggt
    19741 tggaaatgga agtatctggt gaagaagcat cgcgaagggg agttaattac ccgttacata
    19801 gaagccagtg ccgcccagga agccgtcgat gtgttgctct cgctcgaaaa tgaacccgtg
    19861 ctggtaaatg gctggattga caagcatatg aatccggaac tggtcaatcg catgaagcag
    19921 accattcggg ccagacggaa gcgccatttt aatgcggaac accagcatac gcgcaaaaaa
    19981 tcgatcgatc tggaatttat tgtctggcaa cgtctggctg gtcttgcaca gcgtcgcggt
    20041 aaaacgcttt ctgaaacgat tgttcagctg attgaagatg cggaaaacaa agagaaatac
    20101 gcgaataaaa tgtcttctct gaagcaggat ctgcaggcat tgctgggtaa ggaataactg
    20161 acgaaagtca gttcaattta ctaaaggcaa aaaaaacccc gcagcagcgg ggtttttcta
    20221 ccagacgata acttaagcct gcggctgagt tacaacgtct ttgatacctt taacttcgat
    20281 ctctacgcga cgatccggag ccaagcagtc gatcagtgca gcacgctgtt tcacgttgtc
    20341 acaggtgttg ccagtaaccg ggttggattc gcccatacca cgtgcggaga tcttgtctgc
    20401 cgggatacct ttggagatca ggtaatcaac aacagactga gcacggcgct cggacagacc
    20461 ctggttgtaa gcgtcagaac cgatgcggtc agtgtaaccc agaacaacta cggaaccgtc
    20521 tttcggatcc aggttgctca gctggctgta cagctgatcc agagcagcct gaccttccgg
    20581 tttcagggtt gctttgttga agttgaacag aacgtcagac ttcagagtga agtgcttggt
    20641 ctgtacttcc ggtgccggag ctggagcaac taccggagct gcttcgccct gaccgaaacg
    20701 gtaggaaaca cccaggctca gcagaccgtt gtccggacga gtaccgatgg tgtttgcgtc
    20761 accgatgttg ttggtccact ggtattccag acgggtagcg atttcaggag tgatcgcgta
    20821 ctcaacacca cctgcgaaaa ccggagaaac gccagtgtcg tggtctttaa aggatgcgcc
    20881 accaggtacg ttagccttgg tgtctgcacg ccataccata ccacccagac gagtgtagat
    20941 gtccagatcg tcagtgattg ggtaacccag tttagcggtc agctgaacgc cctgagcttt
    21001 gtatgcgccg ttgatgttgt cgcctttgta cggcatacga cctaaccagt cgtaacccat
    21061 ttcaaaacca acatacgggt taacctggta accaccaaaa gcacctgcac ccagttggtt
    21121 ttcgtgggtc ggaccattgt taggaataaa accagtgtca tggtactggg accagcccag
    21181 tttagcacca gtgtaccagg tgttatcttt cggagcggcc tgcgctacgg tagcgaaacc
    21241 agccagtgcc actgcaatcg cgatagctgt ctttttcatt ttttgcgcct cgttatcatc
    21301 caaaatacgc catgaatatc tccaacgaga taacacggtt aaatccttca ccgggggatc
    21361 tgctcaatat taactctacc gatatcttcg gcttatgccg agcacccctg gcgatgtaaa
    21421 gtctacaacg tagttgaaaa cttacaagtg tgaactccgt caggcatatg aaaaaaaagt
    21481 cttgtataag gtatgtttaa tctttttgtc agcgacaatt tacagaaaaa aaacgcggaa
    21541 accgcttcag acaagccttc gcaaggaaaa ttagtcacga ctgaaagcat tggctgggcg
    21601 acaaaaaaag ttccaggatt aatcctaaat ttacttaatg atacaaatta gagtgaattt
    21661 ttagcccgga aagttgtctc gttgtgtgag aggatgcgct taccggacgc ataataaacc
    21721 ccatagcgtt accttcattt gccgcatcaa caagttcagc atgctcttct tcagtcaaat
    21781 catctgtcaa ccaaccgatc accacactgt aattgcccgt gcgtaaagcg cgaaccattg
    21841 actccactgt gtggcaaggg gagagctggc taatctgcat tactttcgtt aagggtagcc
    21901 cagatgcctg aacccattcc cgactcagtt tttgttgcgg tgttaaccag agttgccagc
    21961 gcgattgctg accgagttgc tgtaacaatg gcaacagtag aagttgcgtc atcatgggct
    22021 gatcttcgcg atagacaact tcactgataa gcccggctgt agtgttttcc gtagagacac
    22081 gcgcaatttt acttgctgcg gatgagaacg acgaagaacg atgtgcatag cctgaagtgt
    22141 acataatcaa tccagcccct gtgagttact gtatggatgt gcagtacatc cagtgacaac
    22201 aaagatcaac cctattttcg gaaagagcct cgcaaatttt gtcgttggtg acgggaaaac
    22261 ataaattaat cttgcccctt aagaataagt tgcctatttt cgtagttaac ggatccgtta
    22321 atgtgaatca ttcttttatg ttatgatttt aaaaggaatt ttatgaaaag cctctcctat
    22381 aagcggatct ataaatcaca agaatacctg gcaacgttgg gcacaattga ataccgatca
    22441 ttgtttggca gttacagcct gaccgttgac gacacggtgt ttgcgatggt ttctgatggt
    22501 gagttgtatc ttcgggcttg tgagcaaagt gcacagtact gtgtaaaaca tccgcctgtc
    22561 tggctgacat ataaaaagtg tggccgatcc gttaccctca attactatcg ggttgatgaa
    22621 agtctatggc gaaatcaact gaagctggtg cgtctgtcga aatattctct cgatgcagcg
    22681 ctgaaagaga aaagcacgcg caatacccgg gaaagactga aagatttgcc caatatgtct
    22741 tttcatctgg aagcgattct cggggaggtg gggattaagg atgtacgggc gttacgtata
    22801 cttggggcaa aaatgtgttg gttgcgactg cggcagcaaa acagtctggt gacagaaaag
    22861 attctgttta tgcttgaagg tgccattatc ggcattcatg aagctgcgct cccggtggca
    22921 cgccgccagg agcttgcaga atgggctgac tctcttacgc cgaaacagga gtttcctgcg
    22981 gagcttgagt aatctggcgt tgcagacgac caatctctgg cagtaatgca atcaataacc
    23041 caacttgttg tacgaccaga ggttctttgc tatctgcccg tggttcaagt tgctgcatcc
    23101 gctgtttcag gccagctaat gcctgattga cgcgttcttc atcagcaggt tgatgatgta
    23161 acgcgtcatc aacatagcaa actgcgtcat caagaaacgc cagaatttca ggattagtta
    23221 actgctcccg gtgagcaccg agggctgaga tatagctggt aaacgtatgg ttaaggcaca
    23281 gcaagcgaaa cgcggcttcg cgaatttgcg gggtaacgtt cggttcgctg gacatatttg
    23341 ataccaccga cgccagttca gcatcacggt tgtgtgcatc gcggcgggca atacgatacg
    23401 ccagacggtt atcacgcccc tgatggtatt gctccagtat ggcatcgaga tagcgacagt
    23461 tggcctctgt ggcgcgttcg agcattcgcg gcagattgcg aaacttccag tcaggccaga
    23521 tgtagctcac tgccgcccac gcaatggcac aaccaatcag cgtatcgatt acgcgaggta
    23581 acgctacttc aaaaccttca cccagtaagt taaaacacag tagcaccaaa agtgtgatga
    23641 acatcgttgc atgagcgtat tgcacgttac ggaaggcaaa aaagagcacg ccggtaataa
    23701 ccagcagcac cagctgccct tccagtgatg gcacaaacca cagcacagga atgccaatcg
    23761 cgatacctac cagcgtacca ataatcctta acttcaggcg gtggcgcgtg gcgttatagt
    23821 ttggctggca gacaaacaaa cttgtcagca agatccaata cccgtgatgc attccggtta
    23881 tttgaatgat ggcgtagccg aagcacaaca ccagcgacat tcttaccgca tgacggaaga
    23941 gggcggattc cggcgtgaag tgacggctaa gacgcagcca gatatcactc aatccgtgcg
    24001 ggctgtcatc agcgagctca ttttcgtcat tattacgggg tagtgcctgg gcctgttctg
    24061 attcaattgt tgccagttgg gcatcaatgg cgcgtaaatt gttcagcaaa aatcccagtg
    24121 ttttgagtaa atcggcgggt gcgccgttat cgcgtatccg ctccagcgca gcatcaatat
    24181 gcgtaaaagc gcgctcaaaa tgcggatcat gttgataaga ctgacgcaac aaaatacagc
    24241 gtgacagttg ctggcacgcc tggccctgca tcgacatcag ccgctgaaaa cggaacagca
    24301 cgtcgctgtg gcgaaaatgt tcacgcaatg tttgatactg aatatgagaa gagctggcac
    24361 gctcgtgaat atcctgtgcg acaaagtaat aatgcagcgt gcgacgcgtt ccccgttgac
    24421 cacgatcgcc acgtaagcgg gtcagcagcg agagtttcgt ctgattcaat gtcgccatca
    24481 gctgaccgtt ggcgagagcc aaatcgtaca gcggtgcctg gctttcatct tcaatatcag
    24541 gatcaaacat gcgcgacttg agctcaagat aacgcgccag ttgttcatag caacgcgcca
    24601 ggttgtcctg cagcgggcgg accgggaaca gcagatgacc aataagtgtc aggacgttgt
    24661 accagacggc accggccagc agatacatcg gctgctgata ccagtgctca tacagtgatg
    24721 ttcccaacat agtgtaaatg gcgatcagca atgcaccgaa ggcaattgtt gcatagcgtt
    24781 gacccagacc gccgagcaaa atgaagccgc tggtagagag cgttaagcca atcgcaaata
    24841 gccagggcca gggaaacagc aattctactg aggccgaggc gataaaaaag cagaacagcg
    24901 taatgatgag gttacgcaaa cgtcccgcca gtcggtcatc gagatcggtc agcgccgctg
    24961 ccaccatccc cagcgttagc ggaatcgtca gttttacatc acccagccac cacggaaacg
    25021 ctgtggttcc acaaagcgca ataaaaatac gcgcgtaata cagccaggcg ctgttccaag
    25081 tatagcgttt gagcaaagga cttagcataa aggccatatt agtggattat tcaaaacgac
    25141 gacgtgcatt ggcttcacgc gcagcttgcg ctgtttctac cgataccacg cgacgaccaa
    25201 ccggccatag tgcaatagcg gcaattttaa agttcgcaat gccgacagga atgccaatga
    25261 ttgaaataca ttgtgcgatg cccgttgcaa tgtgcatcag gcataaccac cagccaaaga
    25321 aaatcagcca gaaaatattc aataccgtac cgccagtatt cagcaacaca tttttgccag
    25381 ccgggttcag ttcatcgaca tgaatagctt cattgccata aggcaccaga gacagtttag
    25441 tgatctccca gcaggatcgt gtcagcggta aggtaaaaat cagcacaata ctgaccagag
    25501 tcgccaacag ccagcccaga gtggtggcaa atccgccaag cacaaagttc agaatgttca
    25561 aaacggtacg cataaaacct cgctttactg tggttttcag taatgggcgg caattgtaac
    25621 gtttttttgg gctggagcac gttttctctg acggttacac tgataagaaa taatttcgtg
    25681 tggatctaca gagtcatgga actgaaagcg acaacgcttg gaaaacgtct ggcacagcac
    25741 ccttacgatc gggcggtgat cctcaatgcc gggattaaag tctccggcga tcgccacgaa
    25801 taccttattc ctttcaatca attactggcg attcactgta agcgcggtct ggtatggggc
    25861 gagctggaat ttgtactgcc ggatgaaaaa gtggtgcgtc tgcacggcac agaatggggc
    25921 gagacgcagc gtttttacca tcatcttgat gctcactggc ggcggtggag tggcgagatg
    25981 agcgaaattg cgtctggtgt tttacgccag caactggatt tgattgccac gcgcactgga
    26041 gaaaataaat ggctgacgcg tgagcaaacc tctggcgttc agcaacaaat ccgccaggct
    26101 ttgtcggcgt tgccgttgcc ggttaaccga ctggaagaat tcgataactg ccgtgaggcg
    26161 tggcgtaaat gtcaggcttg gttgaaagat attgaaagcg ctcggttgca gcataaccag
    26221 gcgtataccg aagccatgct taccgagtat gcggattttt tccgccaggt cgagtcttca
    26281 ccgctgaatc cggcgcaggc ccgggcagtc gttaatggcg agcattctct gttagtgctg
    26341 gccggtgcag gaagcggaaa aacgtcggtg ctggtggccc gtgcaggctg gttgctggcg
    26401 cgtggtgaag cgtcccctga gcaaatttta ttgctggcgt ttggtcgcaa agccgctgaa
    26461 gagatggacg agcggattcg cgaacggcta cataccgaag acattaccgc acgcacgttt
    26521 catgcgctgg cgctgcatat tattcagcag ggcagcaaaa aagttccgat agtcagcaaa
    26581 ctggaaaatg ataccgctgc ccgtcatgaa ctctttattg ctgagtggcg caagcaatgc
    26641 agcgaaaaga aagcgcaggc gaagggctgg cggcaatggc tgacggaaga aatgcagtgg
    26701 tcagtgccag aaggtaactt ctgggatgat gaaaaattac agcgtcgcct tgcctctcgc
    26761 ctcgatcgct gggtaagtct gatgcggatg cacggtggtg cacaggcaga aatgattgcc
    26821 agtgcacccg aagagattcg cgatctgttc agtaaacgta tcaagttgat ggccccgtta
    26881 ttaaaagcct ggaaaggtgc gctgaaggca gaaaacgctg tcgatttttc gggccttatt
    26941 catcaggcga ttgtgattct ggagaaaggc cgctttatca gcccgtggaa gcatattctg
    27001 gttgatgaat ttcaggatat ctcgccgcag cgcgcagcgt tgttagcggc attacgcaag
    27061 caaaacagtc agacgacgtt gttcgccgtt ggtgatgact ggcaggcgat ttaccgattc
    27121 agcggtgcgc aaatgtcgct caccaccgct ttccatgaaa actttggtga aggcgatcgc
    27181 tgtgatttag acacgactta ccgttttaac agtcgtatcg gtgaggtggc aaaccggttt
    27241 attcagcaga acccaggcca gctgaaaaag ccgctaaaca gcttaaccaa tggagacaaa
    27301 aaagctgtca cgttattgga tgagagtcaa cttgacgctt tgctggataa gctctctggt
    27361 tatgccaaac cggaagagcg cattctgatc ctggcgcgtt accatcacat gaggcctgcc
    27421 agcctggaaa aagcggcaac acgctggccg aagttgcaaa tcgactttat gaccattcat
    27481 gccagcaaag ggcaacaggc ggattacgtc atcatcgttg gcttgcagga ggggagtgat
    27541 ggttttccgg ctgcggcgcg ggagtcgatt atggaagagg cgctactgcc accggttgag
    27601 gatttcccgg acgctgaaga acggcgttta atgtacgtgg cgctgacccg ggcacgccat
    27661 cgggtatggg cactgtttaa caaagagaat ccctctccct ttgtggaaat actgaaaaat
    27721 ctggatgtgc cggtggcgag aaaaccgtaa gaaacaggtg gcgtttgcca cctgtgcaat
    27781 attacttcag acggtcagcg agataacgct gataatcggg gataagaata tcgaccgcgt
    27841 cgttgaaatg cggcgactgg ataatgaagt ctgccgttgc cacgttggtg gcgaccggaa
    27901 tgttccatac cgtcgccaga cgcagcaagg ctttcacgtc aggatcgtgc ggcacggcat
    27961 ttagtggatc ccagaagaaa atcaatacat caattttccc ttctgagatc aatgcgccaa
    28021 cctgctggtc accccccatt gggccactca acatcgcgtt gacgttcatg ccggtcgcgc
    28081 gggaaatcaa gttaccggta gtgcctgttg catacagtac gtgttgttcc agtaacggtt
    28141 gatgccgttc cacccagctc atcagcattt gtttgcagtg atcgtgtgcc accagcgcaa
    28201 tatgtttccg cgcaggtaaa gtgcgagtcg tcagttccat aatgtacatc cgtagttaac
    28261 tttcctacag attactgtaa gcacttatcg ctgcaagata aagaccgaaa aagcctgcgc
    28321 acaggcacaa aaatctcagg aagatggttg tttttccgcc cattgcagga aagtatttcg
    28381 cgtttgtggg tcagccagtt taaaccaata cttcagccgt tgttctgtga gcacctgaga
    28441 ctgcggtgga atagcatcca gctcggaaac acccgaaagc agcgtactgt catctgccat
    28501 catcgtggca aattgcggta gtgaagcgcg tttcgcggat ttgttatagc gttcaacctc
    28561 gacctcataa tcaatcgttt ttgccgttga ggtaatggcg agaatatcca gctttaccgg
    28621 aatcggcgtc gcatcgccat ccaacaattc aaggcgcggc gcggcatcaa aatggttagc
    28681 ttcccgctca ttctccaggc gaggcaggcg aaaattgacc tggttgatga gctgggtatt
    28741 aaaactgacc accagcggtg gggagatata cagccgttct tcactattgg aaagatgaat
    28801 cgtcttctca acgcgaaaca ctaactgatg cggtccattg tccagttcaa tgctgtccgc
    28861 accgcgcagc agagagctgg aaaccttttt gccgtcgagt accagaagat cgacatcggt
    28921 tgaaagccgc agcgtggtgg caaaaacgga taccggcaaa cataatgcaa tcaaggtggt
    28981 cacgatgccg gttttcatag gtagctcctg tcaaaagacc gcccgaataa taatgtgtca
    29041 aaatttttct ttaatcatac ttactaacat atagacatat ttcccgcttt tgctctcatt
    29101 cattcgtatt agctgcatgg ttggcatgtc ggctttgtcg tacactttgt taaacagtct
    29161 ggaggaaata atgaaagaaa ccgatattgc aggcatttta acgtccactc acaccattgc
    29221 gctggtgggg gcaagcgaca aacccgatcg ccccagctat cgcgtgatga aatatctact
    29281 cgaccagggg tatcacgtca ttccggtttc gccaaaagtt gccggcaaaa cgctgctggg
    29341 ccagcagggg tatggcacgc tggcagacgt tccggaaaag gtcgatatgg tcgatgtttt
    29401 ccgtaattca gaagcggcgt ggggcgtggc acaggaagct attgccattg gcgcgaaaac
    29461 cctctggatg caactggggg tgattaatga gcaagcggca gtactggcac gggatgccgg
    29521 gttaaatgtg gtgatggatc gctgcccggc tattgagatc cctcgcctgg gtctggccaa
    29581 ataaaaaatc cccggaaggc aaaaaccttc cggggatttg ttcagggatt agttacgcag
    29641 acgcggggcc tggagttgtt tgcggatggt ctgcgccagc tcatccatgg atggctgttc
    29701 aggatgttca tcctgcaatt cgctacttag ctgggcttca gccaggtagg tatgaaccgg
    29761 taggccgtta tcgtcctcca tcaccacgtg ataccacgga gcggcgcgaa gctcgtcatt
    29821 caccgccagc tcatcaggcg acggttcaga gagcgaataa accgggtcga tatccacgac
    29881 cactccgaga taacctaaca gggaatggcg gacctgctgg ccgataccga atttgctggc
    29941 aatcatagtc acctcccggg aaatctttac ctttgatata agggtaaaat tccacatttc
    30001 aagttacatg acgcgacagg caaacccttt cagatatagc ccttccgggt aggtagcgat
    30061 caccggatga tcggctgcct gacggaactg ctctataaat tgtacatcac gaccggcatc
    30121 aattgcggca tccgcgatga ttttctgaaa taaatcgctg gtcatcagac cggaacagga
    30181 gaaagtcagg agaataccgc cttcattcag caactgaatc gccagcatgt tgatgtcttt
    30241 ataaccacga cacgcgccca tcaactggct tttattctca acaaacttcg gcgggtccat
    30301 cacgataacg tcaaattttt caccgcgatc gcgataagta cgcagcaatt taaagacatc
    30361 atcacggaca aactcagcct tgctcagatc cagtttgttc agctcaacgt tctgccgtgc
    30421 aatatccagc gcttcctggg aggtatcaac gctgacaacc tggctgcaac cgcccatcag
    30481 tgccgatacg gcgaaaccac cggtatagga gaaacagttc agcacacgtt tattttcaac
    30541 gtagcggcgg gtagccaggc ggctgtctcg ctggtccaga tagtagcccg ttttgtgtcc
    30601 gtgctgaata tccaccagca gcttcatgcc gtgttcttca atcggcagta gggcaggcgg
    30661 caactcgccg gtgacgaggc cctgggtcag ctccatccct tcttttttac gtaccgcaac
    30721 atcgctgcga tcgtaaatcg cacattccgg gtacagcgtt tgcagggcac taattaatgc
    30781 cgcgcgctga tattctgcgc cagcactcag cagttgcagc accagaaaat taccgaaacg
    30841 atcgatagta atacccggca ggccatcaga ttctccggcg attaaacgat agctgtcgag
    30901 gccatctttt tgcgccagcc agtcacgcca tttttgtgct tgttgcaaac ggcgggaaaa
    30961 aaaagcaatg tcgatagact cagacgggtc aaacgtccag acgcgcgccc ggatttgcga
    31021 agctggcgaa taagcgccgc gtgctaacca ttttccctga tgatcaacaa tatcgatggt
    31081 ttcaccgagg ctggctttac cttccatgcg ggcaacggcc ccggaaaaga cccacggatg
    31141 gcgacgaagt aatgattttt cgcgcccttt ggctaacact aaacgtacac tcataattta
    31201 cttttctgtc gatgcaaaga aatgggcgtc attgtccgga gttttccatg gatttgcaac
    31261 gcgtccatta aggataagga gagcagcaat gtcgaaagtc tgcataattg cctggattta
    31321 cgggcgagtt cagggcgtag gatttcgcta caccacacag tacgaagcga aaagactggg
    31381 cttaaccggg tacgccaaaa atcttgacga tggcagcgtg gaagtggttg cctgcggtga
    31441 agaagggcag gtagaaaaat taatgcagtg gttaaagagt ggcggcccgc gttctgcgcg
    31501 tgtagaacgg gtgctcagcg agccgcatca tccctcgggg gaattaaccg attttcgtat
    31561 ccgttaaata cattttaccg gttttggcag gccagcaatt ttggtggctt gcttagccgg
    31621 gcctttcggg aacagtcggt ataaatagcg gctgttacct ttttcttcac caaatttatt
    31681 cgccatcgct tttaccagca tacgaatcgc cggagacgta ttaaattcca ggtagaagtc
    31741 acgtacaaaa cgcaccactt cccagtgttc tggcgacagc gaaatccctt cgttctctgc
    31801 aatcaccact gccagcggtt cactccactg gctgctttct ttgagatagc cttcggtatc
    31861 catttctatc tctttacctt cgaagatcag cataattatt actaccttaa tcagactggc
    31921 ggcagtgtaa caaaaaacaa agccccgcat aaagcgaggc tatgaaagtg ttagcgggtg
    31981 agattaatcg cggctggcga agcccagaat gctcagcagg ctgacgaaga tgttgtacag
    32041 cgaaacatac aggctaaccg tggcacgaat atagttcgtt tcaccgccat gaatgatgtt
    32101 gctggtttca aacaagatag cgccagagga gatcagaatg aagaccgcgc tgatcgccag
    32161 atgcagagca ggcagttgca ggaagatatt agcaaccata ccaatcagta ccaccacaat
    32221 acccgccatc agcataccgc cgaggaacga catatctttg cgggtggtca gcacatatgc
    32281 agagcagcag aagaacacta acgccgttcc gcccagtgcc atagcgatta cgtcacccat
    32341 tccggcagac agataggtgt tcagaattgg tccgaggata taacccagaa aaccggtaaa
    32401 ggcgaatgcg gagataatcc cggtcggctt attcgccgtt ttataggtca ggaacatcaa
    32461 accatacata cccaccagcg tcagaatcag acccggagat ggcagcatca gcaccgtact
    32521 ggcagttgcg gtaatcgccg aaaaggccag cgtcaggctc agcagaaaat aggtattacg
    32581 cagcacctta tgggtgctaa gcagtgatgt acggtcatgt gaagaactaa caatacgatc
    32641 cattgagtca ctctctatga cagatgtaat taattaagca gcataatgat aatgcgtaag
    32701 ggcacccaga agttttaccc atctttacgc atttgatctg gaacaggttt aacagcggat
    32761 tatcaggtca ttaagcaaat ataacgccct gagaattccg acaggcaaaa gaaaaagggg
    32821 ttagcattta gctaacccct tatcttattt ggcggaagcg cagagattcg aactctggaa
    32881 ccctttcggg tcgccggttt tcaagaccgg tgccttcaac cgctcggcca cacttccgga
    32941 atgacgcgca ctataaacat cccgatgcgg cgtgtaaacc cctaatttgt ttgtttgcct
    33001 gaaaaacagc caaaagtgca ttgatagcgt gaaataacag cagattgatc atttcatcac
    33061 catgaattcc ttctctttta ctcgtttagc aaccggctaa acatccccat cgcccggcta
    33121 aagaaaaata ggtccatttt tatcgcaaaa agataaatcc acacagtttg tgttgttttg
    33181 tgcaaaagtt tcactacgct ttattaacaa tactttctgg cgacgtgtgc cagtgcagaa
    33241 ggatgagctt tcgttttcag catctcacgt gaagcgatgg tttgccttgc tacagggacg
    33301 tcgcttgccg accataagcg cccggtgtcc tgccggtgtt gctaggagaa gagacgtgcg
    33361 atatgaataa cgaggaaaca ttttaccagg ccatgcggcg tcagggcgtt acccggcgca
    33421 gctttctcaa atattgtagt ctggctgcca cgtcgctggg attaggcgcg ggaatggcac
    33481 caaagattgc ctgggcgctg gagaacaaac cgcgcattcc ggtggtatgg atccacggtc
    33541 tggaatgtac ctgctgtacc gaatctttta tccgctccgc tcacccactg gcgaaggacg
    33601 tcatcctttc cctgatttcc ctctattacg acgataccct gatggctgcc gccggaaccc
    33661 aggcggaaga agtctttgaa gacatcatca cgcaatataa tggcaaatat atcctcgcag
    33721 tagaaggtaa tccgccgctg ggcgagcagg ggatgttctg tatcagcagc ggtcgaccgt
    33781 ttattgagaa actcaaacgt gccgctgccg gagccagcgc gattatcgcc tggggaacct
    33841 gcgcgtcctg gggctgcgtg caggccgcgc gacctaatcc gacacaggca acgcctatcg
    33901 acaaagtcat caccgacaaa cccattatca aagtacctag ctgcccgccg atcccggatg
    33961 tgatgagcgc catcattact tacatggtga cctttgatcg cttgccagat gtcgacagaa
    34021 tgggtcgtcc actgatgttt tatggtcagc gtatccacga taaatgctat cgccgcgctc
    34081 acttcgacgc cggagagttc gtccagagtt gggatgatga cgctgcccgc aaaggttatt
    34141 gcctgtacaa aatgggctgc aaagggccta ccacctataa cgcctgttcc tccacacgct
    34201 ggaatgatgg cgtttctttc ccaatccagt ctggtcacgg ctgcctgggc tgtgcggaaa
    34261 atggtttctg ggatcgcggt tcgttctaca gccgcgtggt cgatattccg caaatgggta
    34321 ctcattccac cgccgatacc gtcggtttaa ccgcgcttgg cgtggtggca gcagctgttg
    34381 gtgtgcacgc agttgccagc gccgttgacc aacgcagacg tcataaccag caacctacag
    34441 aaaccgaaca tcagccaggc aatgaggata aacaggcatg agcactcagt acgaaactca
    34501 gggatacacc atcaataatg ccggacgccg cctggtggtc gacccgatta cgcgcatcga
    34561 aggccacatg cgctgcgaag tgaatattaa cgatcagaat gtgatcacca atgccgtctc
    34621 ctgcggcacc atgtttcgcg ggctggagat catcctgcaa gggcgcgacc cgcgcgatgc
    34681 gtgggcgttc gttgaacgta tctgcggcgt ctgtactggc gtacacgccc tggcttcggt
    34741 ttacgccatc gaagatgcca tcggtattaa agtgccggac aacgccaata tcatccgcaa
    34801 cattatgctg acaacgctct ggtgccacga tcatctggtg cacttctatc agcttgccgg
    34861 gatggactgg atcgacgtgt tagatgcgct gaaagccgac ccgcgaaaaa cctccgaact
    34921 ggcgcaaagt ctctcctctt ggccgaaatc atcccctagc tatttcttcg acgtacatat
    34981 ttcttcgacg tacaaaatcg cctgaagaaa ttcgttgaag gcgggcagtt ggggatcttc
    35041 cgcaatggct actgggggca cccgcagtac aaattgccgc cagaagccaa cctgatgggc
    35101 tttgcccact atctcgaagc tctcgatttc caacgtgaaa ttgtcaaaat tcacacggtc
    35161 tttggcggta aaaacccgca tccaaactgg attgtcggcg ggatgccttg cgccattaac
    35221 attgacgaaa gcggcgcggt cggggcggtc aatatggaac gcctgaacct ggtgcagtcg
    35281 attatcaccc gcacggcaga cttcattaac aacgtgatga tccccgacgc cttagccatc
    35341 ggtcagttca acaaaccgtg gagcgaaatc ggcacgggtc tttctgataa atgtgtcctc
    35401 agctacggcg cattcccgga tattgccaac gactttggtg agaaaagtct gttgatgcct
    35461 ggcggcgcgg tgattaacgg cgacttcaac aatgtgctgc cagtggattt ggttgatccg
    35521 cagcaggtgc aggagtttgt cgatcacgcc tggtatcgtt atcccaacga tcaggtcggg
    35581 cgtcatccat tcgatggcat taccgacccg tggtacaacc ccggcgatgt caaaggcagc
    35641 gataccaaca ttcagcagct gaatgaacag gaacgctact cgtggatcaa agcgccacgc
    35701 tggcgcggta acgcgatgga agtggggccg ctggcacgca cgttaatcgc ttatcacaaa
    35761 ggcgatgctg cgaccgttga gtcggtcgat cgcatgatgt cggcgttgaa cctgccgctt
    35821 tccggtatcc agtcaacgtt gggccgcagg taagttgcag tatttcttcg acaagctgat
    35881 gaccaacctg aaaaatggca atctcgccac tgcttccact gaaaaatggg aacctgcaac
    35941 ctggccgaca gagtgccgtg gcgtcggctt taccgaagcg ccgcgcggag cgttaggcca
    36001 ctgggccgcc attcgcgatg gcaagattga tctctaccag tgcgtggtgc cgaccacctg
    36061 gaacgccagc ccgcgcgatc ccaaagggca gattggcgct tatgaagcgg cgctgatgaa
    36121 caccaaaatg gcgatccccg agcaaccgct ggagatcctg cgtactctgc acagctttga
    36181 tccgtgcctc gcctgttcaa ctcatgtgct gggcgacgac ggtagcgagc tgatctccgt
    36241 gcaggtgcgt taacagcgaa ggagaatcat catgcaacag aaaagcgaca acgttgtcag
    36301 ccactatgtc tttgaagcac cagttcgtat ctggcactgg ctgacggtgt tatgcatggc
    36361 ggtgttgatg gtcaccggtt actttatcgg caagccgcta ccttccgtca gtggcgaggc
    36421 gacgtatctg ttctatatgg gttacatcag gttaattcac ttcagcgccg ggatgatttt
    36481 taccgtggtc ttgctgatgc ggatctactg ggcttttgtt ggcaatcgtt actcccgcga
    36541 gctgtttatc gtgccggtat ggcgtaaaag ctggtggcag ggcgtgtggt atgaaatccg
    36601 ctggtatctg tttctggcaa aacgtccgag tgctgatata ggccataacc ccatcgccca
    36661 ggcggcgatg ttcggctatt tcctgatgtc ggtctttatg atcatcaccg gttttgcgct
    36721 gtacagcgaa cacagccagt acgctatttt tgcgccgttc cgttatgtgg tggaattttt
    36781 ctactggacg ggtggcaact caatggacat tcacagctgg catcggctgg ggatgtggct
    36841 gattggggcg tttgtgatcg gtcatgtcta catggcgctg cgtgaagaca tcatgtccga
    36901 cgacacggtg atctccacca tggtcaacgg ctaccgtagc cacaaatttg gcaaaataag
    36961 taacaaggag cgttcatgag cgagcaacgc gtggtggtca tggggctggg caacctgctg
    37021 tgggccgatg aaggcttcgg cgtgcgggtg gcggaacggc tgtatgccca ttaccactgg
    37081 cccgagtatg tggagattgt cgatggcggt actcagggac tgaacttgct ggggtatgtc
    37141 gaaagcgcca gccatctgtt gattctcgat gccattgact acgggctgga acctggaagg
    37201 ctgcgaacct atgccggaga acgcattccg gcctatctca gcgcgaagaa aatgagcctg
    37261 catcagaaca gtttctccga agtgctggcg ctggcagata tccgcggaca tctgccagca
    37321 catattgccc tcgtcgggct gcaacccgcg atgctcgacg actacggcgg cagcctgagc
    37381 gaactggcac gggagcaact gcccgctgcg gaacaatcgg cgctggcgca gttggctgcg
    37441 tggggaagtg tgccgcaacc ggctaatgaa tcgcgttgtc tcaattatga ctgtctgtcg
    37501 atggaaaatt acgaaggcgt tcgcttgcgc cagtaccgga tgacacagga ggagcaggga
    37561 tgagcaacga cacgccattt gatgcgttgt ggcaacgaat gctggcgcgc ggctggacgc
    37621 cggtcagtga atcccgtctt gacgactggc ttacgcaagc gccagacggc gtggtgttat
    37681 taagcagtga cccgaaacgc acgccagagg tcagcgataa tccggtaatg attggcgaat
    37741 tactgcacga gtttcccgac tatacatggc aggtggcgat tgctgacctt gagcagagcg
    37801 aagccatcgg cgatcgcttt ggcgcctttc gctttcctgc cactttagtg tttaccggcg
    37861 gaaactatcg cggcgtgctg aatggtattc acccgtgggc ggaactgata aacctgatgc
    37921 gcgggcttgt cgaaccgcag caggagcgtg cctcatgagc gaaacttttt tccatctgct
    37981 ggggccagga acgcaaccga acgatgacag tttcagcatg aatccactgc cgatcacctg
    38041 tcaggtgaat gatgaaccga gtatggcggc cttggagcaa tgtgctcaca gcccgcaggt
    38101 gattgcgctg ttaaacgagt tacaacatca actaagcgaa cgccagccgc cgttgggcga
    38161 ggtgctggca gtcgatctgt taaatctcaa cgccgacgat cgtcacttta tcaatacgct
    38221 tctcggggaa ggggaagtgt cagtgcgcat tcagcaggct gacgacagta aaagtgaaat
    38281 acaggaggcg atcttctgcg gattatggcg ggtgcgcaga cgtcgcggcg aaaagttgct
    38341 ggaggacaaa ctggaggctg gctgcgcgcc gctggcgttg tggcaggcgg caacgcaaaa
    38401 tctcttgccg acagattcgc tgttaccgcc gcccattgat ggcctgatga atggcctacc
    38461 gttggcgcat gagttactgg cacatgtacg taaccccgac gcgcagccgc acagcattaa
    38521 tctgacgcaa ttacccatca gcgaggctga tcggcttttt ctctcacgtc tctgtgggcc
    38581 gggaaatatt cagattcgta ccattggcta tggcgagagc tatatcaact ccacggggtt
    38641 acgccatgtc tggcatttac gctgtacgga caccttaaaa ggcccgttac tggaaagtta
    38701 tgaaatctgc ccaataccgg aagtggtgct ggcagcgcca gaagatttgg tcgactctgc
    38761 gcagcggctt agcgaggtat gtcagtggct ggcggaagct gcaccgacgt aaaaagacgg
    38821 taagtatcgc tttcagtctt atgaatatcg caatcggcga atacctctgg tcgtagagtt
    38881 tcaggataaa gagggagatc taacattatc gggttatttt tctctcttcg cctacaggag
    38941 tgcgcatgtg ggatgtcatt gatttatcgc gctggcagtt tgctctgacc gcgctgtatc
    39001 actttttatt tgtacccctt accctggggc tgattttttt gctggctatc atggaaacca
    39061 tttacgtggt caccggcaaa acaatctacc gcgatatgac gcgcttctgg ggtaagctct
    39121 tcggtatcaa ttttgctctt ggcgtggcta ccggcctgac catggagttt cagtttggta
    39181 ctaactggtc attctattcc aactatgtgg gcgatatttt tggcgcacca ctggcgatgg
    39241 aagcattaat ggccttcttc ctcgaatcca cctttgtcgg gctgttcttc ttcggctggc
    39301 aacggctgaa taaataccag cacctgctgg tgacgtggct ggtggcgttc ggttcaaatc
    39361 tctctgcatt gtggatattg aatgccaacg gttggatgca atacccgacc ggtgcgcatt
    39421 ttgatatcga caccctgcgt atggagatga ccagcttcag cgagctggtc tttaacccgg
    39481 tcagccaggt gaaatttgtg cacaccgtca tggcgggcta cgtgaccggg gccatgttta
    39541 ttatggcgat cagcgcctgg tatttactgc gcggacggga gcgcgatgtc gcattacgct
    39601 cgtttgccat cggttccgtc ttcggtactc tggcgattat cggtaccctg caactcggag
    39661 acagttctgc gtatgaagtc gcgcaagtac aaccggtaaa actggcggcg atggaagggg
    39721 agtggcaaac ggaacctgct cctgcaccgt tccatgtggt tgcctggccg gaacaggatc
    39781 aagagcgtaa cgcctttgcc atcaaaattc ccgcgctgct agggatcctc gccactcact
    39841 cattagataa acccgtgccg ggtctgaaga atttgatggc tgaaacctac ccacgcttgc
    39901 aacgcggacg tatggcctgg ctgttaatgc aggaaatatc tcaaggcaat cgtgagccgc
    39961 atgtgttgca ggcattccgg gagctggaag gcgaccttgg ttacggcatg ttgctctccc
    40021 gctatgcgcc ggatatgaat catgtcacag ccgcacagta ccaggcggcg atgcgtggcg
    40081 cgatacctca ggttgcgccg gtattctgga gtttccgcat catggtgggc tgtggttccc
    40141 tgctgctact ggtgatgctg attgcgcttg tccagacgct gcgtggcaaa atcgaccagc
    40201 atcgctgggt gctgaaaatg gcgctctgga gtttgccgtt gccgtggatt gcgattgaag
    40261 ccgggtggtt tatgaccgag tttggtcgtc agccgtgggc gatacaggac atcttaccga
    40321 catactccgc gcactccgct ttaaccacag gacaactggc tttctcactg atcatgatcg
    40381 taggtcttta caccctgttc ttaatcgccg aagtctacct gatgcagaaa tatgcccgtc
    40441 tggggccgag cgcgatgcag agtgaacaac cgacgcagca acaggggtaa aggagaaaat
    40501 catgtttgat tatgaaacat tgcgcttcat ctggtggctg ctgattggcg tgatcctggt
    40561 ggtctttatg atctccgacg gatttgacat ggggatcggc tgtttgctgc cgctggtggc
    40621 gcgtaatgat gatgaacgcc ggatagtgat taatagcgtt ggtgcgcact gggaaggcaa
    40681 tcaggtctgg ttgatcctcg caggtggggc attatttgcc gcctggccca gagtgtatgc
    40741 agcggcgttt tccggctttt atgtggcgat gatcctggtg ctgtgctcgc tgttcttccg
    40801 tccgctggcc tttgattatc gcggaaaaat tgccgatgca cgctggcgta aaatgtggga
    40861 tgccggtctg gtcatcggca gtctggtgcc gccggtagtc ttcggtatcg ccttcggcaa
    40921 cttgttgctc ggcgtgccgt ttgccttcac accgcaatta cgcgtggagt atctcggcag
    40981 cttctggcaa ctgctgacgc cattcccttt attgtgtggt ttgctcagcc ttgggatggt
    41041 gattttgcaa ggtggcgtct ggttacaact gaaaactgtt ggtgtgattc atctgcgttc
    41101 acagctggcg actaaacgcg ctgcactgtt ggtgatgctg tgctttttgc tggcaggtta
    41161 ctggctgtgg gtcggtattg atggctttgt actgctcgcc caggatgcta acggtccttc
    41221 caatccgtta atgaaactgg tggcagtgct acctggtgcc tggatgaata atttcgtcga
    41281 atcgcccgtt ttgtggatct tcccgctgct ggggttcttc tgcccattgc tgacggtgat
    41341 ggcgatttat cgtggtcgcc cgggttgggg atttttgatg gcatcattga tgcaatttgg
    41401 cgtgattttc acggcaggca tcacgctgtt cccctttgtc atgccgtcaa gtgtgagtcc
    41461 aatctccagc ctgacgttgt gggacagcac ttccagtcag ctgacgctga gcattatgtt
    41521 ggtaatcgtg ctgatatttt tgcccattgt gttgctctac actctctgga gctactacaa
    41581 aatgtgggga cgcatgacaa cagaaactct ccgccgtaac gaaaacgagt tgtactaagg
    41641 agcaggaaca tgtggtattt actttggttc gtcggcattt tgttgatgtg ttcgctctcc
    41701 acccttgtgt tggtatggct ggacctgcgt ctgaaaagtt aacgaacgta ggcctgatgc
    41761 ggcgcattag catcgcatca ggcaatcaat aatgttagat atgaaaagcg gaaacatatc
    41821 gatgaaagcg atcttaatcc catttttatc tcttctgatt ccgttaaccc cgcaatctgc
    41881 attcgctcag agtgagccgg agctgaagct ggaaagtatg gtgattgtca gtcgtcatgg
    41941 tgtgcgtgct ccaaccaagg ccacgcaact gatgcaggat gtcaccccag acgcatggcc
    42001 aacttggccg gtaaaactgg gttggctgac accgcgcggt ggtgagctaa tcgcctatct
    42061 cggacattac caacgccagc gtctggtagc cgacggattg ctggcgaaaa agggctgccc
    42121 gcagtctgct caggtcgcga ttattgctga tgtcgacgag cgtacccgta aaacaggcga
    42181 agccttcgcc gccgggctgg cacctgattg tgcaataacc gtacataccc aggcagatac
    42241 gtccagtccc gatccgttat ttaatcctct aaaaactggc gtttgccaac tggataacgc
    42301 gaacgtgact gacgcgatcc tttgcagggc aggagggtca attgctgact ttaccgggca
    42361 tcggcaaacg gcgtttcgcg aactggaacg ggtgcttaat ttcccgcaat caaacttgtg
    42421 ccttaaccgt gagaaacagg acgaaagctg ttcattaacg caggcattac catcggaact
    42481 caaggtgagc gccgacaatg tctcattaac cggtgcggta agcctcgcat caatgctgac
    42541 ggagatattt ctcctgcaac aggcacaagg aatgccggag ccggggtggg gaaggatcac
    42601 cgattcacac cagtggaaca ccttgctaag tttgcataac gcgcaatttt atttactaca
    42661 acgcacgccg gaggttgccc gcagtcgcgc caccccgtta ttggatttga tcatggcagc
    42721 gttgacgccc catccaccgc aaaaacaggc gtatggtgtg acattaccca cttcagtgct
    42781 gtttattgcc ggacacgata ctaatctggc aaatctcggc ggcgcactgg agctcaactg
    42841 gacgcttcca ggtcagccgg ataacacgcc gccaggtggt gaactggtgt ttgaacgctg
    42901 gcgtcggcta agcgataaca gccagtggat tcaggtttcg ctggtcttcc agactttaca
    42961 gcagatgcgt gataaaacgc cgctgtcatt aaatacgccg cccggagagg tgaaactgac
    43021 cctggcagga tgtgaagagc gaaatgcgca gggcatgtgt tcgttggcag gttttacgca
    43081 aatcgtgaat gaagcacgca taccggcgtg cagtttgtaa tgcataaaaa agagcattca
    43141 gttacctgaa tgctctgagg ctgatgacaa acgaagaact gtctaatgcg tagaccggaa
    43201 aaggcgttca cgccgcatcc ggtcactttc agttttactc tttctcggag taactataac
    43261 cgtaatagtt atagccgtaa ctgtaagcgg tgctggcgcg tttaatcaca ccattgagga
    43321 ttgcgccttt aatattaacg ccagcctgtt ccagacgctg cattgacaag ctcacctctt
    43381 tggcggtgtt caagccaaaa cgcgcaacca gcaggctggt gccgacagag cgtcccacga
    43441 ccgcagcatc gctcaccgcc agcatcgtaa gcgccccatc agcgacgtct tgtgaaaatt
    43501 gtcctgtctg gcaacaatcg cgcccatcta tattgatgga cacgaacgat gaattcccag
    43561 acaacaaaag atattccctg cttccgttct tatttgcctg atgc
//
LOCUS       DAETPZ010000026        40384 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.026, whole genome shotgun sequence.
ACCESSION   DAETPZ010000026 DAETPZ010000000
VERSION     DAETPZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.026"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..789)
                     /locus_tag="K0W59_001615"
     CDS             complement(<1..789)
                     /locus_tag="K0W59_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBI3022700.1"
                     /translation="MTQYSSLLRALAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQVLKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLG"
     gene            1036..1662
                     /gene="gstB"
                     /locus_tag="K0W59_001616"
                     /pseudo
     CDS             1036..1662
                     /gene="gstB"
                     /locus_tag="K0W59_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(1659..2773)
                     /gene="yliI"
                     /locus_tag="K0W59_001617"
                     /pseudo
     CDS             complement(1659..2773)
                     /gene="yliI"
                     /locus_tag="K0W59_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(2884..3267)
                     /gene="bssR"
                     /locus_tag="K0W59_001618"
     CDS             complement(2884..3267)
                     /gene="bssR"
                     /locus_tag="K0W59_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HBI3022701.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3480..4805
                     /gene="rimO"
                     /locus_tag="K0W59_001619"
     CDS             3480..4805
                     /gene="rimO"
                     /locus_tag="K0W59_001619"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBI3022702.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNIKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4852..6180)
                     /locus_tag="K0W59_001620"
     CDS             complement(4852..6180)
                     /locus_tag="K0W59_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBI3022703.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVESINLSTSIGVAHSNKCTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6188..8536)
                     /locus_tag="K0W59_001621"
     CDS             complement(6188..8536)
                     /locus_tag="K0W59_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBI3022704.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNREGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFCVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(8714..9625)
                     /gene="gsiD"
                     /locus_tag="K0W59_001622"
     CDS             complement(8714..9625)
                     /gene="gsiD"
                     /locus_tag="K0W59_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HBI3022705.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9628..10548)
                     /gene="gsiC"
                     /locus_tag="K0W59_001623"
     CDS             complement(9628..10548)
                     /gene="gsiC"
                     /locus_tag="K0W59_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HBI3022706.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10566..12104)
                     /gene="gsiB"
                     /locus_tag="K0W59_001624"
     CDS             complement(10566..12104)
                     /gene="gsiB"
                     /locus_tag="K0W59_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HBI3022707.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVIPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDSGFSFED
                     ADLQ"
     gene            complement(12124..13995)
                     /gene="gsiA"
                     /locus_tag="K0W59_001625"
     CDS             complement(12124..13995)
                     /gene="gsiA"
                     /locus_tag="K0W59_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HBI3022708.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(13982..14947)
                     /gene="iaaA"
                     /locus_tag="K0W59_001626"
     CDS             complement(13982..14947)
                     /gene="iaaA"
                     /locus_tag="K0W59_001626"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBI3022709.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMESVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15151..16386
                     /gene="moeA"
                     /locus_tag="K0W59_001627"
     CDS             15151..16386
                     /gene="moeA"
                     /locus_tag="K0W59_001627"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBI3022710.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            16386..17135
                     /gene="moeB"
                     /locus_tag="K0W59_001628"
     CDS             16386..17135
                     /gene="moeB"
                     /locus_tag="K0W59_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HBI3022711.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17314..17976)
                     /gene="fsa"
                     /locus_tag="K0W59_001629"
     CDS             complement(17314..17976)
                     /gene="fsa"
                     /locus_tag="K0W59_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBI3022712.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            18107..19006
                     /locus_tag="K0W59_001630"
     CDS             18107..19006
                     /locus_tag="K0W59_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBI3022713.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19012..21444
                     /locus_tag="K0W59_001631"
     CDS             19012..21444
                     /locus_tag="K0W59_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBI3022714.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHYELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMAAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21590..22405
                     /gene="ybiV"
                     /locus_tag="K0W59_001632"
     CDS             21590..22405
                     /gene="ybiV"
                     /locus_tag="K0W59_001632"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HBI3022715.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22557..23822
                     /locus_tag="K0W59_001633"
     CDS             22557..23822
                     /locus_tag="K0W59_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HBI3022716.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24063..25655)
                     /locus_tag="K0W59_001634"
     CDS             complement(24063..25655)
                     /locus_tag="K0W59_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HBI3022717.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEGFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            25874..26794
                     /gene="ldtB"
                     /locus_tag="K0W59_001635"
     CDS             25874..26794
                     /gene="ldtB"
                     /locus_tag="K0W59_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBI3022718.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(26852..27970)
                     /locus_tag="K0W59_001636"
     CDS             complement(26852..27970)
                     /locus_tag="K0W59_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HBI3022719.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFQTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGMQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(27967..28434)
                     /gene="mntR"
                     /locus_tag="K0W59_001637"
     CDS             complement(27967..28434)
                     /gene="mntR"
                     /locus_tag="K0W59_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HBI3022720.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28620..28748
                     /gene="mntS"
                     /locus_tag="K0W59_001638"
     CDS             28620..28748
                     /gene="mntS"
                     /locus_tag="K0W59_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HBI3022721.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29020..30603
                     /gene="opgE"
                     /locus_tag="K0W59_001639"
     CDS             29020..30603
                     /gene="opgE"
                     /locus_tag="K0W59_001639"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBI3022722.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYKTFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30651..31166)
                     /gene="ompX"
                     /locus_tag="K0W59_001640"
     CDS             complement(30651..31166)
                     /gene="ompX"
                     /locus_tag="K0W59_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HBI3022723.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGLNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31219..31284)
                     /locus_tag="K0W59_001641"
     CDS             complement(31219..31284)
                     /locus_tag="K0W59_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022724.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31519..32406
                     /gene="rhtA"
                     /locus_tag="K0W59_001642"
     CDS             31519..32406
                     /gene="rhtA"
                     /locus_tag="K0W59_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HBI3022725.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32705..33208
                     /gene="dps"
                     /locus_tag="K0W59_001643"
                     /gene_synonym="pexB"
     CDS             32705..33208
                     /gene="dps"
                     /locus_tag="K0W59_001643"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HBI3022726.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33612..34358
                     /gene="glnH"
                     /locus_tag="K0W59_001644"
     CDS             33612..34358
                     /gene="glnH"
                     /locus_tag="K0W59_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HBI3022727.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEVYKKWFSTEPK"
     gene            34497..35156
                     /gene="glnP"
                     /locus_tag="K0W59_001645"
     CDS             34497..35156
                     /gene="glnP"
                     /locus_tag="K0W59_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HBI3022728.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35153..35875
                     /gene="glnQ"
                     /locus_tag="K0W59_001646"
     CDS             35153..35875
                     /gene="glnQ"
                     /locus_tag="K0W59_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HBI3022729.1"
                     /translation="MIEFKNVSKHFGSTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36136..38360
                     /gene="ybiO"
                     /locus_tag="K0W59_001647"
                     /pseudo
     CDS             36136..38360
                     /gene="ybiO"
                     /locus_tag="K0W59_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38357..39283)
                     /gene="rlmF"
                     /locus_tag="K0W59_001648"
     CDS             complement(38357..39283)
                     /gene="rlmF"
                     /locus_tag="K0W59_001648"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HBI3022730.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39559..39819
                     /locus_tag="K0W59_001649"
     CDS             39559..39819
                     /locus_tag="K0W59_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBI3022731.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            40084..>40384
                     /locus_tag="K0W59_001650"
     CDS             40084..>40384
                     /locus_tag="K0W59_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBI3022732.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT        10047 a        10680 c         9825 g         9832 t
ORIGIN      
        1 ccccagcact acggagatta aacgcatatc accctgggta gccgaagcaa ccagattata
       61 tcccgcacct gccgtggttc ctgtcttcat gccatcaaca ttcagattgc tgctccacag
      121 cagacggtta cggttaggct gacgaatttt gttgaaggtg aactctttct ctttatgaat
      181 ggcgtactct tccggcacat cgtggatcaa tgctttaccc agcaatgcca tatcgcgcgc
      241 ggtactgaac tgccccggcg catccaggcc gtgcaccgtc tggaaggtag tgttggtcag
      301 ccccagtttt ttggcataac cattcatcag accaataaag gactcctggc tcccggcaac
      361 gtaatcggcc agcgcaatac aggcgtcatt accggactgg ataatcacac ctttgttcaa
      421 gtctgccacc gaaacctgat cgcccggttt gaggaacatt accgatgaac cacgcagtgc
      481 cggatttccc gtcgcccagg catctttacc gaccgtcacc atatcggtga gtttaatctt
      541 atcggcctta agcacctgcc caaccacata gctggtcatg attttagtca ggctcgcggg
      601 atccagtttc tcatccgcat tgccttctgc cagcacttta ccgctggcgt aatccattaa
      661 aatccatgca cgcgcatcca cgctcggcgc ctcaacggtt tgttcggccg cgaatgccgt
      721 tggggcaaaa aggaataaaa atgcagaacc cgctgcaaga gcacgaagga gagaggagta
      781 ttgcgtcata aatgccaccc gagtatccat tcaaaaaaaa tacgttatat cgccgtatga
      841 taagttcggt gattaataac gcacacacaa acttaaagaa accgtaagtt ggtaaagttt
      901 ttaaagttta tgcaatatcc tgccgcttcg ctgcttctgg tgtcattttt ttgccagctg
      961 ttgcttaaaa gttaacttta cttcaccatc ggagtcctgt tcagtctgtg gctgcctgtc
     1021 ggaaaagggg tgagtatgat tacgctgtgg ggtcggaata attcaactaa cgtaaaaaaa
     1081 gtattgctga cgctcgaata actggaacta ccttatgagc aaattctcgc gggccgtgag
     1141 tttgggataa atcacgatgc tgattttctg gcgatgaacc ctaacgggct ggtgccgttg
     1201 ttacgtgacg acgaaagtga tctcattctt tgggaatcaa acgccattgt ccgctatctg
     1261 gcggcacagt acgggcaaaa acgcctgtgg atcgactcac cggcacgtcg tgcggaagcg
     1321 gaaaaatgga tggactgggc aaaccagacg ctcagtaatg ctcatcgcgg gatcctgatg
     1381 ggattagtca gaacaccacc ggaagagcgc gatcaggccg ccattgatgc cagttgcaaa
     1441 gagtgcgacg ccctgtttgc cctgctcgat gcggaactgg caaaagtaaa atggttctct
     1501 ggcgacgagt ttggtgtggg cgatatcgct atcgcaccgt tcatctacaa tttgtttaac
     1561 gttggcctga cctggacacc gcgtccaaat ctgcaacgct ggtatcagca actcactgaa
     1621 cgccccgcag tgcgcaaagt ggtgatgatt cccgttagct aattgcgtgg gctaacttta
     1681 agtaattccc cactggactc gtcggtgaga acgtataaat aaccgtcggg tccagtgcga
     1741 acatcacgaa ttcgctgccc tctgtccgtt aaaatacggc catcttctgt cactttgtcg
     1801 ccgttgacgc tcatcacaat gacatcttta tctttcagcg cccaataaat aatttttgct
     1861 gccactgggg gaatttatcg ctgttataga aggccatgcc gctcacagcg ggcgaatctt
     1921 tccagtaaaa aacaggttgc tcggtcccgg cgacgatctc ccctttcgct tccggtatct
     1981 taaagcctga atagttgatt ccccaggttg ccagcggcca gccgtagttt ttgccttttt
     2041 gcgggatatt aatttcatca ccaccgcgcg ggccatgttc attcagccac agtgcattac
     2101 tccacggatt catcgccatt ccttgcggat tacgaatgcc ataagaccag atctcggcgc
     2161 gcgcaccgga ttcctttata aaaggattat catccgggat ttcgccctgg tcggtcagac
     2221 gcaccagttt gccctgtaat ttatccagat cctgcgccgt cgggcgctga ttgttttcgc
     2281 ccagagcaat aaaaagataa cctttaccgt cgaataccag ccgcccgcca aaatggttgc
     2341 cggtagacag ttttggcatc tggcggaaga cggtgcggaa gtcggtcact tttgagagat
     2401 catcacttaa gcggccataa cccacagcag ttccggcttt gccatcatcg ccaacttcgg
     2461 aataacttaa ccagatgcgg cgagactgag caaaatcagg cgctaaaacc acgtccagca
     2521 ggccgccctg cccgtgcgcc caaacgtccg gaactccgga aagcggcgca gataatcctt
     2581 ttcctgcttg ccagtgacgc aactcgccgc cgcgcagagt gattaacata ccgtgattat
     2641 cgggtaaaaa ggccagtgcc cagggatggt cgagtttgtc ttgcagtact tcgacattta
     2701 ccgttgcagg agccgcccag agagcggaag aaagacaaat aaggggcaca aggaaaaagg
     2761 attgtcgatg cataacgcct ccggattgac tctggcttaa agcgtagtca gtggaggaga
     2821 taacaaattc atttttacaa aaacttaaac atgaaggggg agacgctttc tcccccttag
     2881 ttttcaggcc ttctcaagca tggcgtgctt ctgcaggctc tggatactca gcgttaagct
     2941 catcagacaa ttttcaagct tatcggcgtt gacggtaata acagtcgggc aatcatggtg
     3001 cccactcatc aaacatactg cggctgtcgc taatgcttct tcagcatgat gaagagcact
     3061 ccactcttcc tgatccagat gaagattcaa ccgcagcgat ttatcgtgca gttcgcgatt
     3121 cagtttaaaa aagttatctc gtagatgatt gctttcgctg acggacatgt atccttttgc
     3181 cttcctcagt tgcacgtatg cagcgaggtc aacgcgtgcg tcgatcaacc ggttcagcag
     3241 atcgattcgc tgtctgtcaa cgaacatact tcgatcctcc tctttccagc cttgttgcaa
     3301 ttaaatagta tggctatttt ttgagcaaaa tgctgtctag gatcaattct tcacgccgaa
     3361 ttaacaaaat ttacatttcc ttcgcctccg ccagcgaatg agattgcgat acaaatttta
     3421 tccagaactc tgtagaatcc ctgccctgac ctttcctgta actgaatatt ttttaagcta
     3481 tgagcaaagt aactccccag ccgaaaatcg gctttgtttc ccttggctgt ccgaaaaacc
     3541 ttgtcgattc agagcgtatt ctcaccgaac tccgcactga aggttatgac gtggtaccga
     3601 gctatgacga tgcggacatg gtgatcgtca acacctgcgg ctttattgac agcgcggtac
     3661 aagaatcact ggaagccatt ggtgaagcgt tgaatgaaaa cggcaaggta attgtgaccg
     3721 gttgtctggg ggcaaaagaa gatcagatcc gcgaagtcca cccgaaagtg ctggaaatca
     3781 ccgggcctca tagctatgag caggttctgg agcacgttca tcactacgtg ccaaaaccga
     3841 aacacaaccc attcctgagc ctggtgccag aacaaggtgt gaaactgacg ccgcgtcatt
     3901 atgcctatct gaaaatttcg gaaggctgta atcaccgctg caccttctgc attattccgt
     3961 ctatgcgcgg cgacctggtg agccgtccga ttggcgaagt gttaagtgaa gcgaaacgtc
     4021 tggtagatgc gggcgttaaa gagattctgg tgatctcgca ggatacttcc gcctatggcg
     4081 ttgatgttaa acatcgtact ggcttccaca acggcgagcc ggtaaaaacc agcatggtca
     4141 gcctgtgcga acagttatcg aaactgggga tctggacacg tctgcactac gtttaccctt
     4201 atccgcatgt ggacgacgtc atcccactga tggcagaagg caaaatcctg ccgtatctgg
     4261 acattccgtt gcagcacgcc agcccgcgca ttctcaaact gatgaagcgt ccgggttctg
     4321 tagatcgcca actggcgcgc atcaaacagt ggcgcgaaat ctgcccggaa ctgaccctac
     4381 gctcaacctt tattgtcggc ttccctggcg agacggaaga agatttccag atgctactcg
     4441 acttcctgaa agaagcacgt ctggatcgcg ttggctgctt taaatacagc ccggttgaag
     4501 gtgcagacgc caatgccctg cctgaccagg ttccggaaga agtgaaagaa gaacgctgga
     4561 accgtttcat gcagctgcag cagcagattt ccgccgagcg cctgcaagag aaagtgggcc
     4621 gtgaaattct ggtgattatc gacgaagtgg acgaagaagg cgcgattggt cgcagcatgg
     4681 cagatgcacc ggaaatcgac ggcgcggttt atctcaatgg tgaaaccaac attaagccgg
     4741 gtgatatcct gcgtgtgaaa gtcgagcacg ccgatgagta cgatttgtgg ggtagccggg
     4801 tttaattaat tcaggccggg caaaatcatt tgtccggcct gcgaataatg attactgcca
     4861 gctaatctgt gccctgccgt ttttcttact cttatacagc cgctcatccg ccagcatttt
     4921 caagcgttcg acggtagtac atttatttga atgcgctaca ccgatactgg tgcttaaatt
     4981 aatgctttcc acctggaaat gaacgatttc ttttagtatc gatagcaact cttccagcgt
     5041 gccgccagta taaatcaccg caaattcgtc acctccgaag cggtagactt tacctttatc
     5101 taccgggatc tgtgatttaa ggattttcac cactttcatc aacacttcat cgcccagcaa
     5161 atgaccgaag gtgtcgttaa tgtttttgaa tttatcgacg tcaaacagac acaaataacc
     5221 gcctttttcc ggcagcgctt ttaaatcttc atcaaaggat tttctgccca ttgctttggt
     5281 caggtcgtcc aggaacgccg agttatacat actgtagagt tttttgacat cggcagccga
     5341 aaagaaaagc ccgccatcca gtacgcgttc aaaatggtta ttaatgatgg cttctttctc
     5401 cacaatacca cgattaatca ctctgcgtac cagatagagg caactggcac ccgatatcac
     5461 tgccaccaat gcggcaaaga aaataatcaa tgtacaaacc cgcaaataaa catctttttt
     5521 atacgcaaat acgtacaatc cggtcggcaa ataggcgaag tgctccatcg cttccgaaac
     5581 ggtgaagatg gcgtttttag cattatattc atcaccggtc accggtttac cgttcttaaa
     5641 tagagagaac atcaggttct cgctgtccat cgccagcgat tttgacagta aagaaaaacg
     5701 ctccagacca agcaaaaata tcagactacg attgtgagtt accggataga ggtaatagac
     5761 gaaggtttta ccatcaaaag agataaaccc tgtatttttg acatgtcgat acttcacaac
     5821 ctgcataata tctgcacaat aatttttctc gaactccttt acctgaatac attcgtcact
     5881 ggaaatcaga taaggaccga catcgtacaa ataagagggg gaaaccgagt cggcgtaagc
     5941 tgtgcgcaaa ccaaccaaca gctgttcctc cgtgggattt agcgtgttga taagatcaat
     6001 tgccacgcta cgccgatatt cagcaatatt tgccaccgca aaagcagtat caataacgga
     6061 ctgtttcagg ctatacaccc gttcctttac catttgagtg tagatcccgc aggcaactgc
     6121 tgaaatcatg ataaaaatca gcagactgac tgtaagtacg aacttattga ttctggacat
     6181 acgtaaacta ctcttttact aattttccac ttttatccca ggcggagaat ggcatgggtt
     6241 tctggtacag atacccctga gcctgaacac atcctaattt aattaattcg cgcgcaacgt
     6301 cttcgctctc tacgccttcc gcaatcaccg ggatccggtt atagcgtgcc agatcgaaaa
     6361 taatgcgcac aatttttccc gcagtaccga aaatatcgcc ggtaaattgc gagtccacct
     6421 ttatgctgtc tggattaatt tcataaaaac gtttcaggtt tgacaaaccg gtgccaaagt
     6481 catcaatcga tatccgtacc ccttgtttac gcagttgttc aatattgaga taaaaatgtt
     6541 cattaattcg ctcgatcacg gtttcagtga tctcaacaca gaggctgttc gccttcaggc
     6601 cattttcttt catgatggtg gtaaaacgct catgaaagtg cggttcgttt aactgccagg
     6661 gcgaaacatt aatatgcagt aagaaatcat ccttcagcga cgcacgttga ctaaaatgtc
     6721 tgaattcacc cattgccgtt ttaataatct gataacccag ctcattgata aacccgatat
     6781 cttcagcaat ggtaatgaac ttcagcggtg aaatgatccc ccgctccgca gaaacccaac
     6841 ggcacaacgc ctccgcgctc agaatggcgc gggtgttaat atcaacgatc ggttgataga
     6901 cgaggaatag ttcgccaatt tccattgcat ggcgaatatt acgcgcaatc cagatattct
     6961 cttctatttc gttgagtttc gcgtcatcac agaaaataaa ctcggagaac atatggtcac
     7021 ggatactgga aatcgccagt ttcagttttt ctgcgtaact ccaggcgttt tccccctgca
     7081 aaggttcaca aatggcagac ttatggatgc gcagattgcg gtagccaaag ctgctgtcaa
     7141 aatcgtttac catcgctatt ttacgggtaa atgtctgtac attttcccga cacagaagta
     7201 aatagagatc ggcattgaat gtcacgatat cgcagcattg cgcatatttc tcggcaattc
     7261 gctgacacaa tttcctgacg attttattgc gttcatcaac acaaaacacc gcttccacgg
     7321 cttccagacc acacagtttg atgatcccca cagccagata ctgattattg ttggactcaa
     7381 taatcgccat atttttaata aaaccgcttt cctgatcgta agagatcagt gagagcaatt
     7441 tatttgaccg ttcaatttcg gcatctttca tttccgcgat cgaatttttg atgctgataa
     7501 tctctttcgc atcgccttcg cgaattttca gagcctcgat ctgatccgtt gagtcgggca
     7561 aaaacgccag cgtattaacc agctcggtca tttcagaaaa ttgccgacgg aacacacgag
     7621 tggtcatgaa atataccacc agcaacacga taataatgaa cgcaaacggt agataaaaaa
     7681 gtttatctga tgcgttcaat aaattaccgc caggataata aaaatagatt tcgaacaatg
     7741 cattatttgg cactgctgat gtctttgcaa tatactccgt atcgacagaa tcaataatat
     7801 tataattacc ttctctatta taaataatca gtttttcacg agcgagttga ttcttggcaa
     7861 tgatctcatg accttgtttc aaaaatagaa cttcatcatt agcgctgatt tgatcaagat
     7921 actcgttcag ataccgccag tttaaatcga catgaatttt atctaccgct aacaaatcgt
     7981 gatctttgcc gttgatacgt agcgtaaaag agatacattt tttacccgga cgatcaaagc
     8041 aattgtatgc cggattccag ctgttgttag agtcatctga agccaccgac cacggtcgtt
     8101 tcgatgggtt aaaatcggca aaaataaccc gccctgactc gtcgacaagc ggtccattta
     8161 cttgttcctg atgataaaca acgattttat cattcgccct ccgcgaaaag agataatatt
     8221 tattatcatc aagtaccagt cccaccgagt ctaaaaatgg catcatttcc attttcagaa
     8281 tcacggcgcg ttgaatctcc tctgtatttt ttaataaata cagtgagtcc tttaatgtca
     8341 gggccgaacg ttcaatatct ttaaagaaat agccaacgtt tttctgaata agttcaaagc
     8401 ggctatcggc acgttttacc gcgcgttccg ataccagttg atagttaatc atgaaaaaaa
     8461 gaccaataaa tgacagtgct gccaataaaa ataaaattat cagccttatc tttatctttt
     8521 cgtataaact cagcatgtta atacatttaa caaaataact atctgaacgc tgtgccgctg
     8581 caaaaatagt cccttgctgc ggctttttaa ccaaattacc cgattctaca aaatcagcag
     8641 aaagttacaa cacttgctta gggcaataat ttcctgtgag gtgattaccc tttcaagcaa
     8701 tattcaaacg taactatcct ttaattttcg gatccagcgc atcgcgtaaa ccatcgccca
     8761 acaaattgaa cgccagtacg gtcagaaaaa tagccagggc cggaaaaaca gcgacatgcg
     8821 gcgcgataac catatccgct cgtgcctcat tgagcattgc tccccactct ggtgtcggcg
     8881 gctgcgcacc taggccgaga aatgacaggc tggcggcaga gataatcgag gtgccaatgc
     8941 gcatggtgaa aaacaccacg atagaagaga cggtcccagg caggatatga cgcaataaaa
     9001 tggtcatatc gctggcacca atactgcgtg ctgactcaat aaaggtttgc tgtttcaaca
     9061 ccagcgtgtt gccgcgcacc aggcgggcaa acgcggggat ggaaaaaatg gcgactgcga
     9121 taatcacgtt agcaatgccg cttcccaaca ccgcaacaac agcgatcgcc agtaaaatac
     9181 ccgggaaggc aaacagcaca tcgcaaatgc gcatgatcag ccgatcccac cagccttcat
     9241 aatatccagc caacaagccc agcaacgtcc ctatcgccac accgataaac acggcaaaca
     9301 cgcccgccgc cagcgagatt tgcgcaccaa ccaggacacg gctgaaaatg tcgcgcccca
     9361 gtgaatcgac gccaaaccag tgctgcaaag aaggtccgtt attcagattg tcataatcaa
     9421 aataattttc ggcgtcatag ggagcgatcc agcgtgcaaa aatggccacc acaatcaata
     9481 aaataacgaa taatgcggcg gtcatcgcca tatgctgacg gcgaaatcgt cgccagaatt
     9541 catgccacgg tgtacgtacc tggtcaggtt tgaccagtgg catggcgttt aacaccgcct
     9601 gacgtcgcca gttaaatagt cgcatcctta cttgtacctg atagccgggt taatggcggc
     9661 gtaaagcaca tccaccacta agttgataag aataaattcc agcgagaaaa gcagaatttc
     9721 cgcctgaatc accgggtaat cgcgcatttc tacagagtca acgagtaagc gtccaagtcc
     9781 gggccagttg aaaactttct ccaccacgat agaacctccg agcaaaaagc caaactgtaa
     9841 gcccatcatg gtcactaccg ggatcatcgc gttgcgtagc ccgtgtttga ggacaaccca
     9901 ggtttcgctc acccctttcg ccctcgcggt acgcatataa tcttcgctta aaacgtcgac
     9961 aaatgacgca cgggtaaagc gcgccatcac ggcggccact gccgcgccga gcgtcaggga
    10021 gggtaaaatg tagtgctgcc aactgtctgc tcccacagta ggcagccagc ccagttcaac
    10081 ggagaatacc tgaattaaaa acatccccag cgcaaatgcc ggaaacgaga tccccgacac
    10141 cgcaatggtc atactcaatc gatccggcca acgattacgc cagacggcgg cgataattcc
    10201 cgccgccata ccaaatatca ccgcccagac catactggtt atggtcagcc acagcgttgg
    10261 cataaagcgg ctggcaatct catcggcaac cggacgacgc gacaccatcg acaggccaaa
    10321 atccccctgc acagcattgc tgatatagtg ccagaactgg tgatacagcg gctgatccaa
    10381 ccccagctgc tgacgcacca gttctataac ctgcgcatca gcttcgggcc cggcaatcaa
    10441 tcgcgccgga tcgccgggca gcatgtggac aaataaaaac accagcaccg agacgataaa
    10501 cagcgtcgga atcaacccca gtaatcgttt gataacgtaa ttaagcattc cactccctgc
    10561 gttgcttatt gcaaatccgc gtcttcaaag ctgaagccgc tgtctggcat gatccaaaaa
    10621 ccggtcaggt ttttactgtg tgccgacacc agtttttcta ccaccagcgg gatccacggc
    10681 gattcttgcc agatgatatc ctgcgccgcc ttatataagc gggtcttttc cgccggatca
    10741 ttcgttttca gtgcctgagc caggaagtca tccacctgtt tattgctgta aaacgcggta
    10801 ttaaacagcg tcggtggcca gttttgcgag gcaaacagcg gcgatagtgc ccagtcggct
    10861 tcgccggttg aagccgacca gccagtgtag aacatccgca cgccgctctc tttttgccct
    10921 ttaccttcaa cttctgccgc ccgctgtccg gcatccatcg cggtcacctg ggctttaatc
    10981 ccgacctgcg ctaactgctg ctgggtaaat tgcagcactt tctgcgcggt gctgtggtta
    11041 tgtgacgacc acagcgtggt actgaaaccg ttgggatatc ccgcctcttt cagtaattcg
    11101 cgcgctttca ctggatcgta aggccacggt ttataacttt gcgcgtaggc gatacttggc
    11161 ggtatcacac cagtagctgg cgttgcatag cccgcaaagg caactttcac cagcgccgga
    11221 cggttaatgg cgtaattcag cgcctcacgg accttcgggt tatcgaacgg cttttgcgtc
    11281 acgttcatac tgatataacg ctgcataatt gacggactgg ccatcaactc gatatttttg
    11341 tttttctcca gcagtgtggc ctgctcgtag ggaatgggga aagcaaactg cgcttcaccg
    11401 gtttgcagca ttgccgcgcg ggtgttgtta tccgccaccg gacgccaggt tatgctgtcc
    11461 agtttgggca atcctggctg ccagtaaccc gcgaattttt tcaccttcac aaaatcggtc
    11521 tgattccagg tatccagttc atacggtccg gttcccaccg gatgaaaacc aatctccttg
    11581 ccatattttt ccagcgctgt cggtgaaatc atcgcggtcg ccggatgggc aagaatatta
    11641 ataaacgctg agaacggctg tttgagggta atctttaccg ttgtcggatc gatcgcctcc
    11701 gttttggcaa tattcttata caggttatag cgtttaagat gattcgccgg atcgctggcc
    11761 cggtccagat tcgcttttac cgccactgcg ttgaaatcgg tgccatcctg gaatttaatt
    11821 ccttcccgca atttcacggt gtaagtaatg ccgtcatcgg aaacggtata actctccgcc
    11881 agcacgtttt tcagtttcat ctctttatcc agaccgaaca gcccctggta aaacgatttc
    11941 gctacggcct gagataacgt gtcattcgcg tcatacggat cgagcgtggt gaaattcgat
    12001 cctaccgcca ccaccacatc tttggcagcg aatgcacaag atgccatcaa cgctgtcgca
    12061 atgcccagcg ccactaaccc actacggtgt acagctcttg ccattttatt ctccgcctga
    12121 atgttatcta cgcatgaatg cgtattctga ttgtggttgt gcgacgtaat gccccggccc
    12181 gacgcattgc aacgagacgg ctgccacctc ttcgccacgc agatgaatat tgctgggaag
    12241 atcgtccgac agcagtacac gctgcggtcg ttgtcgggac ggttcagcga ccggaactgc
    12301 cgccagtaat ttacgcgtat aaggatgctg cgggttttcg aagaccgcgc accgtgaacc
    12361 aatttcaaca atttgcccga gatacatcac cgccacacga tgactaatcc gctctaccac
    12421 agccatatcg tgggagataa acagatacgc aatgccgaaa tcacgctgga gatcgagcaa
    12481 caagttgata atctgcccgc gaatagaaac atccagcgcc gaaacggctt cgtcggcaat
    12541 gatcactttt ggattcaatg ccaacgcgcg agcaatgcag atgcgctggc gctgaccgcc
    12601 ggaaaactca tgcgggtaac gccaggcatg ttcaggtaac aggcccacgc gctccagcaa
    12661 ccacgcaacg cgtgcagccg cgtctttacc tggcaataaa ccgtgtacac gcagcggttc
    12721 gataatcgaa tcaccgatgg tctgacgtgg gtccagcgaa gcgtaagggt cctgaaaaat
    12781 aaactgaata tcccggcgca atgcctgaag tttgccaggt gacaaggtat cgattcgctg
    12841 accgttaaag ataatttcgc cgccctgcga ttcgaccagg cgcagcaacg cccgcccggt
    12901 agtggattta ccgctgccag actcgcccac cagcgatagc gtttcgccag gccagagatc
    12961 aaaactgact ttctcaacgg catgcacttc ccgcgttacg cgattcaaca aaccgctgcg
    13021 caaagggaaa cgggtgacaa gattacgcac ccgtaaaaca ggttcgccat ccaccaccgt
    13081 tttctgctcg atggggggtt cctgtttcgc tggatgttca agcgatatca atgggaaacg
    13141 tcggggataa tctaaccctt tcatcgcacc aagttgcgga acagcagcta acagcgcacg
    13201 ggtgtaagga tgttgcggtg catgaaaaat ctgttcgacg gtacccgttt ccaccgcctc
    13261 gccctgatac atcaccagta cccgatcggc aatctctgcc accacgccca tatcgtgagt
    13321 gataaagata acgcccatcg acatctcttt ttgcaatact ttgattaatt gcaggatctg
    13381 cgcctgaata gtgacatcca gcgcggtggt tggctcatcg gcaatcagca ccgccgggcg
    13441 gcatgacagc gccatcgcaa tcatcactcg ctggcgcatc ccgccagaga gttgatgcga
    13501 ataacgtgaa agaatggttt gtgcctccgg aatgcgtacc tgatccagca tccgcttcgc
    13561 ctcgaccatc gcttcttcac gactggcgtt ctgatgcaga cgaattgatt cggcaatctg
    13621 ttcacccaca gtaaatactg ggttcagcga tgtcatcggc tcctgaaata tcatcgccat
    13681 atccgcaccg cgcacatggc gcatttgtgc agcgctctgc tcgctaagtt caatcacatc
    13741 acgactgcgc cgccgcaaca gcattttatc gcactgtact aaaccgcccg cctgttccaa
    13801 cagacgcatc aatgccagcg cagtcactga cttaccggag ccggattcgc ctacaattgc
    13861 cagcgtctca ccgcgttgca gactaaaaga gagattgcgg accgcagcta ttttctgctg
    13921 gtcctgcata aaggcaatat taagattttc aaccgccagc acattaccgg catcaagttc
    13981 atcactgtgt ggcaacggtg tccccttttt cacggtagat accggtggtt ggcgtatcgc
    14041 ctgcgtagcc ccaggcgcga tacattcctt cggtgttaaa cggtagcgcg acattccctt
    14101 catggtcgat agcgattaag ccaccgctac cgccaagcgc agggagtttt tccattacta
    14161 cccgctcgca ggcttccgcg agacttaatc cgccgtaatc cattaacgcg gcgatgtcat
    14221 atgccgccag cgcgcggatg aagacttcgc ctgtgccggt acaagaaacc gccacactgg
    14281 cgttattggc gtagcatccg gcacccacta agggactatc gccaactcgt ccgggtaatt
    14341 tattggtcat tccgcctgtg gacgtggctg ccgccagatt gccgtctaaa tccaacgcca
    14401 cggcccccac ggtgcccatt ttctgttttt catccagtgg cgcaccgcta tggtcgagga
    14461 ctgttgcccc ttcctcgcgc gctgccagta gttgttcata acgcaaaggc gtggagaaaa
    14521 tctccggtga gacgctctcc atgccatgag caaacgcaaa attttctgcc ccttcgccaa
    14581 tcatcatcac atgcgggctt tgctccatca ccagccgggc ggcaagaacc ggattacgca
    14641 gatgactaac gcccgccacc gcaccggctt tcagggtgtt accatccatc acacaggcgt
    14701 ccagttcatg ggtttcatca cgcgtaaaga cagcgccaat tccggcgtta aacagtggac
    14761 actcttccag cagacgcacc gcttccgtca ccacatccag cgcactttcg ccggcttcca
    14821 gcattttctg cccggtttca acaatggcag acaacgcctc gatgtagcgt aattcctgtt
    14881 gcagactcat ctgcgcgcgg ctaattgcac ctgcgccacc atgaattgca atgactgctt
    14941 tgcccatagt attcgtccat taagggtggc tcgccacttt tgctataaat atcagaattt
    15001 ttatgaatta ctcattcaat ttttgagtat agaaagatgg atatggcatg taaaggcagg
    15061 agccaacgct acgcccgagg tggcatacag tctatggcgt ttttctgaca taataggcaa
    15121 attcgatttt gcctccgcag gagtgttttc atggaattta ccaccggatt gatgtcgctc
    15181 gacaccgcgc ttaatgagat gctttctcgc gttaccccac tgaccaccca ggaaacgctg
    15241 ccactggttc agtgttttgg tcgtattctg gcgagcgatg tcgtttcgcc actggatgtc
    15301 ccggggtttg ataactccgc tatggacggc tacgcggtgc gtttagccga tattgcctcc
    15361 gggcaaccgc tgcccgttgc cggtaaatcc tttgccggtc agccatacca tggtgaatgg
    15421 cctgcgggta cctgcattcg tattatgacc ggtgcgccgg tgccggaagg ctgcgaagcg
    15481 gtggtgatgc aggagcagac tgaacaaacg gacaatggcg tgcgttttac tgctgaagtg
    15541 cgtagcgggc aaaatattcg ccgtcgcggt gaagatatct ctgcaggtgc ggttgttttc
    15601 ccggcgggaa ctcgcctgac taccgcagag ctgccagtaa ttgcttcact ggggattgcc
    15661 gaagttccgg tgattcgtaa agtgcgtgta gcgctttttt ctaccggtga tgaactccag
    15721 ttgcccggtc agccgctggg cgacggccaa atctacgaca ccaaccgtct cgccgtacac
    15781 ctgatgttag aacagctggg ttgcgaggta atcaacttag gaattatccg cgacgatcca
    15841 catgccctgc gcgccgcatt tattgaagcc gacagccagg cggatgtagt gatcagttcc
    15901 ggcggtgttt cagtgggtga agcggattac accaaaacga ttcttgaaga gctgggggag
    15961 atcgccttct ggaagctggc gattaaacca ggtaaaccgt tcgcattcgg taaactcagc
    16021 aatagctggt tctgcggcct gccgggcaac ccggtttcag cgacgctgac cttctatcaa
    16081 ctggtacagc ctttgctggc aaaactaagc ggcaacaccg ccagcggcct gcccgcgcgc
    16141 cagcgcgtac gcacagcgtc ccgcctgaaa aaaacgccag gacgtcttga tttccagcgc
    16201 ggcgtgctgc aacgcaacgc cgacggtgaa cttgaagtga cgaccaccgg acatcagggt
    16261 tcacatatat ttagctcctt tagtctcggc aactgcttta tcgtgctgga acgcgatcgc
    16321 ggcaatgtgg acgtgggcga atgggtggaa gtagaaccgt ttaacgcgtt gttcggaggc
    16381 ctgtaatggc ggaactcagc gatcaggaga tgctgcgcta caaccggcaa atcattctgc
    16441 gcggttttga ttttgacggt caggaggcgc taaaagattc tcgtgtgctg gtagtcggcc
    16501 tcggtggact cggctgcgca gcttcgcagt atctggcaag cgccggtgtc ggcaacctga
    16561 cgctgctcga cttcgacacg gtttcgctct cgaatctgca acgccagaca ctgcacagtg
    16621 atgccacggt cgggcaaccg aaggtggaat ccgcccgtga cgccctgacg cggatcaacc
    16681 cacatatcgc gattacgcca gtcaatgcac tgctagatga cgcagaactt gcagcattga
    16741 ttgctgaaca cgatctggtg ctcgactgta cggataacgt tgcggtacgt aatcaactga
    16801 acgcaggctg ttttgccgcg aaggtgccgc tggtttccgg cgcggcaatt cgtatggaag
    16861 ggcaaatcac ggtcttcact tatcaggacg gcgaaccgtg ctatcgctgc cttagccgtt
    16921 tgtttggcga gaacgcgtta acctgcgtag aagcaggcgt gatggcaccg ttgatcggcg
    16981 taattggttc gttgcaggca atggaagcga tcaaactgct ggcaggttat ggcaaaccgg
    17041 ccagcgggaa aatcgtcatg tacgatgcga tgacctgtca gtttcgcgaa atgaaactga
    17101 tgcgcaatcc ggggtgtgag gtgtgcgggc agtaattgcc acctccaggc ctgataagac
    17161 gcgacaagcg tcgcatcagg catttgctcc agacgccaga tgcggcgtga acgccttatc
    17221 tgccctacgg ttcgggcaca gatttccaag cgcggcaggc atcggtgcac tccgccacgt
    17281 catcagcaat ttatgaggat gcagaacgtc cggttaaatc gacgttctgc caaacgctcc
    17341 ctgccagtcc tgctcaaact tcgccacagc ggcttccacc gccggatagc taatcatctg
    17401 ttgtgccaca tccagtggca gagtaattga ttcacatccg gccagtaagc agtccagcgc
    17461 ctgacgcggg gttttgaaac tcgctgccag cactttcgcc tgcggcgcat gcattttcaa
    17521 taactggtgt aagtcggtca cagtctgaat gccgctaccg ccctgagcat caatacgatt
    17581 aacgtaaggc gcaacatatt ccgcacctgc cagcgccgac agcagccctt gtgccgcgcc
    17641 atataccgcg gttcccagcg tcggaatccc ttccgctttt aacatcttaa tcgctgccag
    17701 cccctcggct gtcaccggaa ctttcaccac gatatccgca ataatagaac gcagcttaag
    17761 cgcgtcattc accatccctt cagcagtggt agccattacc tgggcaaaca gacgcccctg
    17821 accgcccatc gcttcatgaa gttgcggaag cacaacatcc agcggttttt tacccgcggc
    17881 gataatgctt gggttagtgg tcacacccgc cagcggaaaa atacgtgaca gcgccttcac
    17941 cgcaacaacg tctgaagtat ccagatacag ttccataacc atcctcaaaa tgtacttttt
    18001 gtctgaatgt agactatcac gggaaaactc tctgaacagt tgacgcacat caatctaact
    18061 ttcattcgaa agtattttaa tgtttgaacg aaacagagag gcaactatga ttttcaatat
    18121 tcagcgctac tcgacccatg atggccccgg tatccgcacg gtcgtatttc ttaaaggctg
    18181 ttcgctgggc tgccgctggt gtcagaaccc ggaaagccgc gcccgtacgc aggatctgct
    18241 gtatgacgca cgactgtgtc tggaaggctg cgagctgtgc gctaaggccg cgccggaagt
    18301 gattgagcgc gcgctgaatg gtttgcttat tcatcgggaa aagttaaccc cggagcatct
    18361 gacggcgtta accgactgct gtccgacaca ggcgttaacc gtgtgtggtg aagtgaaaag
    18421 cgttgaggag atcatggcga ccgttctgcg cgataaaccg ttttacgatc gcagcggcgg
    18481 cggcttaacg ctttcgggtg gtgaaccctt catgcagccg gaaatggcga cggcgctgct
    18541 gcaagcctgc cacgaggcag gcattcatac tgcggtagaa acctgtctac atgtgccgtg
    18601 gaaatatatc gccccttctc tgccctatat cgatctgttt cttgccgatt taaaacacgt
    18661 tgccgacgcg ccgtttaaac agtggaccga cggtaacgcc gccagggtgc tggataacct
    18721 gaaaaaactc gccgcagcgg gcaaaaaaat cattatccgc gtgccgctga ttcagggctt
    18781 taatgccgac gaaacctctg taaaagccat taccgatttt gccgccgacg agctgcacgt
    18841 tggcgaaatt cattttctgc cctaccacac gctgggcatc aacaaatatc acttacttaa
    18901 tctgccctat gacgccccgg aaaaaccgct tgatgcgcca gagctgctcg attttgccca
    18961 gcagtatgcc tgccagaaag ggttaaccgc gaccttacga ggataacaac catgaccaca
    19021 ctgaaactgg acacgctcag cgaccgcatt aaagcgcaca aaaatgcgct ggtgcatatt
    19081 gtgaaaccgc cagtctgtac cgagcgcgcg cagcactata ccgagatgta tcaacaacat
    19141 ctcgataagc cgatcccggt acgtcgcgcg ctggcactgg cgcatcacct ggcgaatcgc
    19201 accatctgga tcaaacacta tgagttgatc attggcaacc aggcaagcga agttcgcgcc
    19261 gcgccgatct tcccggaata tactgtctcg tggatcgaaa aagagattga tgatctggca
    19321 gatcgtcccg gtgctggctt tgcggtgagc gaagagaaca aacgtgttct gcatgaagtg
    19381 tgcccgtggt ggcgcggtca gacagtacag gatcgctgct atggcatgtt taccgatgag
    19441 caaaaaggtc tgctggcgac cggcatcatt aaagcggaag gcaatatgac ctccggcgat
    19501 gcgcacctgg cggtcaattt cccgctgctg ctggaaaaag ggcttgatgg tctgcgtgag
    19561 aaagtagcgg aacgtcgctc gcgcatcaat ctgacggtgc tggaagattt gcacggcgaa
    19621 cagttcctga aagcgattga tatcgtgctg gtggcagtca gtgaacacat tgaacgtttc
    19681 gctgccctgg cgcgtgcaat ggccgcgacc gaaacccgcg aaagccgtcg cgatgaactg
    19741 ctggcgatgg cagaaaactg cgatcttatc gcccaccagc cgccgcagac tttctggcag
    19801 gcgctgcaac tgtgttactt catccagttg attttgcaga ttgaatctaa cggtcactcg
    19861 gtgtcgtttg gtcgtatgga tcagtatctc tacccgtact atcgccgcga cgttgaactg
    19921 aaccagacgc tggatcgcga acacgccatc gaattgctgc atagctgctg gttgaaattg
    19981 ctggaagtga acaaaatccg ctccggctcc cactcaaaag cctctgcggg aagtccgctg
    20041 tatcagaacg tcaccattgg cggacaaaat ctggttgatg gtcaaccaat ggacgcggtg
    20101 aatccactct cttacgcgat cctcgaatcc tgcggtcgcc tacgttccac tcagcctaac
    20161 ctcagcgtgc gttaccatgc aggaatgagt aacgatttcc tcgacgcctg cgtacaggtg
    20221 atccgctgcg gcttcgggat gccggcattc aacaacgacg aaatcgtgat cccggaattt
    20281 attaaactcg gtattgaacc gcaggacgct tatgactacg cagcgattgg ttgtatagaa
    20341 accgccgtcg gtggcaaatg gggctatcgc tgtaccggca tgagctttat caacttcgcc
    20401 cgcgtgatgc tggcggcgct ggaaggcggg cgtgatgcca ccagcggcaa agtgttcctg
    20461 ccacaagaaa aagcgttgtc ggcaggtaac ttcaacaact tcgatgaagt gatggccgcg
    20521 tgggatacgc aaatccgtta ctacacgcgt aaatcaatcg aaatcgaata tgtcgtcgac
    20581 accatgctgg aagagaacgt gcacgatatt ctctgctcgg cgctggtgga tgactgtatt
    20641 gagcgagcga aaagtatcaa gcaaggcggc gcgaaatatg actgggtttc tggcctgcag
    20701 gtcggtattg ccaacctcgg caacagcctg gcggcagtga agaaactggt gtttgaacag
    20761 ggcgcgattg gtcagcaaca gctggctgcc gcactggcgg atgacttcga tggcctgact
    20821 catgagcaac tgcgtcagcg gctgattaac ggtgcgccga agtacggcaa cgacgatgat
    20881 accgtcgata cgctgctggc tcgcgcttat cagacctata tcgacgaact gaaacagtac
    20941 cataacccac gctacggtcg tggtccggtt ggcggcaact attacgcggg tacgtcgtct
    21001 atctccgcta acgtaccgtt tggcgcgcaa actatggcaa cgccggacgg gcgtaaagcc
    21061 cacaccccgc tggcagaagg cgctagtccg gcctctggta ctgaccatct cggccctact
    21121 gcggtcattg gctcagtggg taaactgcct acggcagcga ttctcggcgg cgtgttgctc
    21181 aaccagaaac tgaatccggc aacgctggag aacgaatctg acaagcagaa actgatgatc
    21241 ctgctgcgta ccttctttga agtgcataaa ggctggcata ttcagtacaa catcgtttcc
    21301 cgcgaaacgc tgctggaggc gaaaaaacat ccggatcagt accgcgatct ggtagtgcgt
    21361 gtcgcgggtt attccgcctt cttcaccgcg ctctctccag acgctcagga cgatatcatc
    21421 gcccgtactg aacatatgct gtaatctctt cggcccggct gcggcgtcgc cgggctaaat
    21481 cattcacatc atcaatttca tccttacttt cattcgaaat ataatttgtg ctctgcgtca
    21541 cattgttatt acactgttac ggtttcattt tcgagccagg agcacaccta tgagcgtaaa
    21601 agttatcgtc acagatatgg acggtacttt tcttaacgac gccaaaacgt acaaccaacc
    21661 acgttttatg gcgcaatatc aggaactgaa aaagcgcggc attaagttcg ttgtcgccag
    21721 cggtaatcag tattaccagc ttatttcgtt ctttcctgag ctaaaggatg agatctcttt
    21781 tgtcgcggaa aacggcgcac tggtttacga acatggcaag caactgttcc acggcgaact
    21841 gacccgacat gaatcgcgga ttgttattgg cgagttgcta aaagataagc aactcaattt
    21901 tgtcgcctgc ggtctgcaaa gtgcatatgt cagcgaaaat gcccccgaag catttgtcgc
    21961 actgatggca aaacactacc atcgcctgaa acctgtaaaa gattatcagg agattgacga
    22021 cgtactgttc aagttttcgc tcaacctgcc ggatgaacaa atcccgttag tgatcgacaa
    22081 actacacata gcgctggatg gcattatgaa acccgtcacc agtggttttg gctttatcga
    22141 cctgattatt cccggtctac ataaagcaaa cggtatttcg cggttactga aacgctggga
    22201 tctgtcaccg caaaatgtgg tagcgattgg cgacagcggt aacgatgcgg agatgctgaa
    22261 aatggcgcgt tattcctttg cgatgggcaa tgctgcggaa aacattaaac aaatcgcccg
    22321 ttacgctacc gatgataata atcatgaagg cgcgctgaat gtgattcagg ctgtgctgga
    22381 taacacatcc ccttttaaca gctgacctct ctaccggagc catgcgctcc ggttttccct
    22441 tctttttaca tcctgtacat cacatcaaat cattaaactt gcattaactt atttataact
    22501 tgtattgtca catgcaaatc agcatagttt cgaatgaaag ttaatgcgag gtggttatgg
    22561 cttctacttt taccagcgac acattgcctg ccgatcacaa agcagctatc cgtcagatga
    22621 agcacgcgct gcgggcgcag cttggcgatg tccagcagat ctttaatcag ctaagcgatg
    22681 acattgccac gcgagtggct gaaatcaacg cactcaaagc acagggcgat gccgtctggc
    22741 cggtgctgtc ttatgccgat atcaaagcag gtcatgttac tgcagagcag cgcgaacaga
    22801 ttaaacgtcg cggttgtgcg gtgataaaag gccatttccc ccgcgaacaa gcgctaggct
    22861 gggatcagtc gatgctggac tatctgaacc gcaaccgctt tgacgaggtc tacaaaggcc
    22921 ccggcgataa tttcttcggg acgctcagcg cttcacgtcc cgagatttac cccatctact
    22981 ggtcgcaggc gcaaatgcag gcccgccaga gtgaagaaat ggcgaatgcg cagtcgtttc
    23041 tcaatcgtct gtggacattt gaaagtgatg gaaagcaatg gtttaacccg gatgtgagcg
    23101 tcatctaccc tgaccgtatc cgccgccttc cgcccggaac gacctccaaa ggtcttggag
    23161 cgcataccga ctccggggcg ctggaacgct ggctgcttcc agcgtatcag cacgttttcg
    23221 ccaacgtctt taatggcaat ctggcgaaat acgatccctg gcatgcggca catcgtacgg
    23281 aagttgaaga gtacacggtg gacaacacca ccaaatgttc cgtgtttcgg acattccagg
    23341 gctggactgc gctctcggat atgctgcctg gtcaggggtt gctgcacgtc gtgcccattc
    23401 ctgaagccat ggcgtacgta ctgttacgtc cgctgcttga tgatgtgccg gaggatgaac
    23461 tgtgcggcgt agcgcccgga agagtgttgc cggtatcaga gcaatggcat ccactgttaa
    23521 ttgaggcgtt aaccagcatt ccaaaactcg aggccggaga ctccgtctgg tggcactgcg
    23581 acgtcatcca ttccgttgcc cccgttgaaa atcaacaggg ttggggcaac gtgatgtaca
    23641 ttcctgcggc accgatgtgc gagaaaaatc ttgcctacgc gcacaaggtg aaggccgcac
    23701 tggaaaaagg cgcatcgccg ggcgacttcc cgcgcgagga ctatgaaaca aactgggaag
    23761 gacgctttac gcttgccgac ctcaacattc acggtaagcg agcgttgggc atggatgttt
    23821 gagagttaat ggaagtgaat tgatgtggga ttaagaaaat cgattcgacg ttactcagat
    23881 tgctgacaag tgcgctttct taatgcctga tgcggcgtta acgccttatc cggcctacaa
    23941 agttctgcag attcaatata ttacaggtac tgcgtaggcc tgataagcgt agcgcatcag
    24001 gctattttgc atttgtcatc agccgtcgtc accccgacag tatcggggtg acggataatc
    24061 acttactcga tccctttact acgcaggtaa tcttcgtaat taccgctaaa gtcgatcacg
    24121 cgttccgggg tgatttccag aatgcgggtc gccagggagc ttacgaactc acggtcgtgt
    24181 gaaacaaaga tcagcgtgcc ctgatacagt tccagcgcca tgttcagtga ctcgatggat
    24241 tccatatcca ggtggttggt cggttcgtcc atgatcagaa tgttcggctt ctgcatcatt
    24301 aacttaccaa acagcatccg ccctttttcc ccaccggaaa gcactttagc tggctttttg
    24361 atgtcgtcct ggctgaacag caaacgaccg agaatgctgc gtaccgcctg ctcgtcatcg
    24421 ccttcctgct tccactgact catccattcg aacacggtca gatcattttc aaactcatat
    24481 tcgtggtcct gggcgtagta accaatgcgc gcgttttcag accattttac ggtgccgctg
    24541 tccggttgca gatcgcccac cagcgttttc agcagcgttg atttaccgac gccgttggta
    24601 cccagtaccg caagtttttc accgacctcc agcagcaggt tgagattttt aaacagtgga
    24661 ccgttatcaa accctttggt cagaccttcc acttccagcg cgttacggaa cagtttctta
    24721 tcctgttcaa aacggatgaa cgggttctga cggctggagg ctttcacctc ttccagtttg
    24781 attttatcaa tctggcgcgc gcgcgaagtt gcctggcgag atttcgaggc gttggcgcta
    24841 aagcggctaa cgaaagattg caactcagca atctgcgctt tcttcttggc gttatcggcc
    24901 agcagacgtt cacgcgcctg ggtcgccgcc gtcatgtact catcgtagtt acccggataa
    24961 acgcgcagct cgccgtaatc cagatccgcc atgtgggtac agaccatgtt aaggaagtga
    25021 cggtcgtgcg agatgatgat catggtgctg tcacgctcgt tcagcacctg ttccagccag
    25081 cgaatggtgt cgatgtcgag gttgttggtc ggttcgtcga gcaggagaat atccggctca
    25141 gcaaacagcg cctgcgccag aagcacacgc agcttccagc caggagcaac ttcactcatc
    25201 gggccgtagt gctgttccac tggaattccc acgccaagca acagttcacc ggcgcgagct
    25261 tccgcagagt aaccgtccat ttcgccgtat ttcacttcca gatcggccac tttatagccg
    25321 tcttcttcac tcatttccgg caaagcatag atgcggtcgc gctcctgctt cacttcccac
    25381 aactctttat gccccatgat caccgtatcc agcacagtga acccttcaaa ggcaaactga
    25441 tcctgacgca gcttaccaat gcgctcgttg ggatcgaggg aaacgttacc cagcgtcggc
    25501 tcaaggtcgc cgccgagaat cttcataaag gtggatttac cactaccgtt cgcgccaatc
    25561 aggccgtaac ggttgccgcc gccgaatttg acggaaatgt tttcaaacaa cggcttactg
    25621 ccgaactgca tggtgacgtt actggaaact aacacaggcg tatcctgaaa agagatatga
    25681 caaaccgcgc attatgccat aagtctgaaa gaagatcctg ctcctcgctt aaggtctaca
    25741 ctcaagccaa aggcaaaaaa tgtgatttcg tacacatctg atttcactgt gagctggaat
    25801 gaacttataa tgcgcttcca atactctaat attctcaacc caatggcctg ccaggcacaa
    25861 aatctcgctt aacatgaata tgaaattgaa aacattattc gcagcggcct tcgctgttgt
    25921 cggcttttgc agtaccgcct ctgcggtaac ttatcctctg ccaaccgacg ggagtcgcct
    25981 ggttggtcag aatcaggtga tcaccattcc tgaaggtaac actcagccgc tggagtattt
    26041 tgccgcggag taccagatgg gactttccaa tatgatggaa gcgaacccgg gtgtggatac
    26101 cttcctgccg aaaggcggta ctgtactgaa cattccgcag cagctgatcc tgccggatac
    26161 cgttcatgaa ggcatcgtca ttaacagtgc agagatgcgt ctgtattact atccgaaagg
    26221 gaccaacacc gttatcgtgc tgccgatcgg cattggtcag ttaggcaaag atacgcctat
    26281 caactggacc accaaagttg agcataagaa agcaggcccg acctggacgc cgaccgccaa
    26341 aatgcacgca gagtaccgcg ctgcgggcga accgctaccg gctgtcgttc ctgcaggtcc
    26401 ggataacccg atgggtctgt atgcactcta catcggtcgc ctgtatgcta tccatggcac
    26461 caacgccaac ttcggtatcg gcctgcgtgt aagtcatggt tgtgtgcgtc tacgtaacga
    26521 agacatcaaa ttcctgttcg agaaagtacc ggtcggtacc cgcgtacagt ttattgatga
    26581 gccggtaaaa gcgaccaccg agccagacgg cagccgttat attgaagtcc ataacccgct
    26641 gtctaccacc gaagcccagt ttgaaggtca ggaaatcgtg ccaattaccc tgacgaagag
    26701 cgtgcagaca gtgaccggtc agccagatgt tgaccaggtt gttcttgatg aagcgatcaa
    26761 aaatcgttcc gggatgccgg ttcgcctgaa ttaatcgtca aaacgtaaaa caaaggcgga
    26821 ctcataatcc gcctttttta tttgccagac cttaccaggc cgggatcata actaacaaaa
    26881 cgtatcccac cagcgccgcc cacaacagca tcggtattga atagagatgg aaacgccacc
    26941 agatgcggcg atcgttcgcc atacgtagcg caatcaaatt tgccagcgat cctggtaata
    27001 aaccaaagcc ccccacgttt accgcccatg ccagtaataa agacggaggt acataattga
    27061 gcagcaaaat ggtactcggc acgttactga tcacctgcga taaaccgact gccgttaacc
    27121 ataacccggg ttcagataaa tgactcacgt tacccaacac gccttgcaac gctggaagct
    27181 gggtcagtaa atggacgtcg ataaacatcg ccataaacac cagcagcaac gtccagtcca
    27241 cactcaacac cacgcgacgc gccagcagca caaagcccgc cgccacaatc accagtcccc
    27301 acagctcttg tttgaactcc agcgccgtca gaaaaacgat atacagcccc agacaattcc
    27361 acaccagccg cggtttccac tccggtgttt gcatccccgt atggtattgc agcgcctttc
    27421 cagggaaaca acaccagcac aacagcagga gtgtcagcat cattgcgcca gccagcggtg
    27481 ccatttgggc aataaatccg gcaaacgaaa gaccagaacg tccccagata agaatatttt
    27541 gcgggttgcc aattggcgtc agtagcgaac cagcgttgac tgccagcgcc tcaaaaataa
    27601 tcagccgatt aaccgggatc tcacacagtc tttttagcgt gatagtcagc ggaacaacaa
    27661 taaacagcgc gacatcgttg gtctgaaagg tagaaagcag cgccgccgcc agcaccataa
    27721 acatcgccag ccgacgctcc gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa
    27781 aataaccgct taactccaca cctttggtca gcagcatcag gccgcttaag gtgatgatgg
    27841 tgtgccagtc gatagcagca ggccaggatt tcggtgcaaa gggcacgaaa aagcttaatc
    27901 cgataccaac aagaattaat aactgaaaaa aacgatcgcc ttgcagcgtg cgtaaaaaag
    27961 gcaggctcat ttggcaccgt gtttctgggt aaacaaacga aaagcgtcga gcgtctcttc
    28021 actaacatgg tgctccatgc cttccgcgtc gcgacgggcg atttccggac tgacgcccaa
    28081 caccaacaag aaattttcga ctatctgatg tcgctcgcgg ctttcctgcg ccagcttctc
    28141 tccttctgcc gttaaaaaca cgccacgcca ggggatcatt tcaatcagcc ccatggttgc
    28201 cagccgctta agcattttag ccaccgtcgg ttgcgaaact cccagacgag cagccatgtc
    28261 cacctgacga gcttccccca cttccctgat caagtcagaa atcagctcaa cgtaatcatc
    28321 aataagctcg cgccgatgcg cctctctgac ctggcggaac ccttcaacgt gctcttccac
    28381 gttcactaac tgcgtcactt tttttgctgt tggcgtacct gcgcgacgac tcattgtgct
    28441 tcctcattat ggtgacgcat acatagcgtt ctcatttcgt gcgcacattg taaaccagag
    28501 ttgcgaaggt acaaaaaatt aacgttttag caatagctat ataatatagc ctgtgctata
    28561 tctgtatgta atgcaatcat ccctcaagga tcgacgggat tagcaagtca ggaggtctta
    28621 tgaatgagtt caagaggtgt atgcgcgtgt ttagtcattc tccctttaaa gtacggttaa
    28681 tgctgctctc tatgttgtgc gatatggtca acaacaaacc gcagcaagat aaaccttcag
    28741 ataaatagcg gcgtcgcggt acgccgcttc actcctgctt ttatgcaggc ataacgcgtt
    28801 ttggtctgaa aaaaaccact ttttgtcgga tttgcaatcc ccttcgcaaa agatttgttc
    28861 gtcagtagtt gacctgaacg gcggctcgct ctatcttctt gcagccctgc gtatattgcg
    28921 gctcgcggat gcggccccct ttcccctctt cacgcactct tgcaggtatt gacccttgac
    28981 gccagggtaa gcacatggcg tttgttacca tagtggcata tgaatttaac cctcaaagaa
    29041 tcgcttgtta cccgtagccg ggtatttagc ccgtggactg cgttctactt tttacagtcg
    29101 ctattaatta acctcggctt aggttacccc ttcagtttgc tctacaccgc tgcgtttacg
    29161 gctattttgc ttttgctatg gcgaacattg cctcgcgtac aaaaagttct ggtcggtgtc
    29221 agttcgctgg tggcggcttg ttatttccct tttgctcagg cctacggcgc gcctaatttc
    29281 aatacattgc tggcattgca ctccaccaat atggaagagt cgaccgaaat cctgacgatt
    29341 tttccgtggt acagctacct ggtcggctta tttatttttg cgctcggcgt aatagcaatc
    29401 aggcgaaaaa aagagaatga aaaagcgcgc tggaatacct tcgacagcct gtgtctggta
    29461 ttcagtgtgg cgacattttt tgttgctccc gtgcaaaacc tggcctgggg tggcgtattt
    29521 aaactgaaag atactggcta tccggtattt cgttttgcta aggatgtcat cgtcaataat
    29581 aacgaggtga ttgaagagca agaacggatg gcaaaacttt ccggaatgaa agatacctgg
    29641 acggtcactg ccgttaagcc gaagtatcag acctatgtgg tagtgatcgg tgaaagcgcg
    29701 cgtcgcgatg ccctcggtgc ctttggcggt cactgggaca ataccccgtt tgccagcagc
    29761 gttaacggtt tgatatttgc tgactacatt gccgccagtg gctccacgca gaaatcgctt
    29821 ggcttaacgc tcaatcgcgt agtcgatggc aaaccacagt ttcaggataa ctttgtcacc
    29881 ctagcaaatc gcgcgggctt ccagacctgg tggttttcca accagggcca aatcggcgaa
    29941 tacgatactg ctatcgccag catcgccaaa cgtgcagatg aagtgtactt cctgaaagaa
    30001 ggtaattttg aagcagataa aaacacgaaa gacgaagcgt tactggatat gaccgctcaa
    30061 gtgctggcgc aagagcactc gcaaccgcag ctgattgttc tgcatctgat gggctcgcat
    30121 ccgcaggcct gcgacaggac acaaggcaaa tacaaaacct ttgtgcaatc gaaagaaacg
    30181 tcgtgctatc tctataccat gacgcaaacg gacgatttac tgcgcaagct gtacgatcag
    30241 ttacgcaaca gcggcagcag cttctctctg gtttactttt ctgaccacgg tctggccttt
    30301 aaagagcgcg gcaaagacgt gcaatacctt gcccatgatg ataaatatca gcaaaatttc
    30361 caggtgcctt ttatggtcat ttccagcggc gataaagcgc atcgggtgat taaagcccgc
    30421 cgctcagcca atgacttctt aggctttttc tcgcagtgga cgggaattaa agcgaaggaa
    30481 ataaatatca aatacccgtt tatatctgag aagaaagccg ggccgatata catcaccaac
    30541 ttccagttac agaaggtgga ttacaaccat ctcggaaccg atattttcga cccgaaacct
    30601 taaaaacaaa aatccgcccc gagaggcgga tttttatatc accaaagtga ttagaagcgg
    30661 taaccaacac cggcaatcca ggtgcctacg tcaacgctac gaatacggct ctgctcgtaa
    30721 gagaagtcca gagcaacgtt ttccatcggg ttgaactgca gaccagcacc gtaggagaaa
    30781 ccgtagtcgc tggtgtcgtg tttgtaggtc gggtattcag tggtctggaa tttaccataa
    30841 cccacaccca ctacaccgta gatgcttgcc cagtcgttaa tgcggtaagc cggaccagca
    30901 gtgatgccgt agtactggtt tttgttgtag tcaccagagc ttgcagtacg gcttttctcg
    30961 gtgtaagtga aagaaccgat cacacccagc gggctgttgt cttcttcata gcggtatttc
    31021 aggtttaaac cgcccatttt gttcatttgg ccctgagcgt cgctctgtgc gtaaccgcca
    31081 gttacggtag aagtcgcagc tacggaagta cctgcggtga aagccagaac tgcggccagt
    31141 gctgaaagac atgcaatttt tttcataacc acctcaaatg tgattcaaat aagtcctaag
    31201 ttttaaatat atcaaaaatt aatgggaaac tcttcgcgat ttgtgatgtc taacgggcca
    31261 tttcatgtaa cagaacgttt ccatacaccg ctatccatct aaatttaaat cactttttca
    31321 gagaactgcg taagtattac gcatgttttc cctgtcattc atccagatta ttcctaatca
    31381 ccagactaat gattccatca atcctggcgc attttagtca aaacggggga aaattttttc
    31441 aacaaatgct caaccagcat tgggtatatc cagtacactc cacgctttac ttaagtctag
    31501 atatttgtgg gagaaaggat gcctggttca ttacgtaaaa tgccggtctg gttaccaata
    31561 gtcatattac tcgttgccat ggcgtctatt cagggtggag cctcgttagc taagtcactt
    31621 tttcctctgg tgggcgcacc gggtgtcact gcgctgcgtc tggcattagg aacgctgatc
    31681 ctcatcgcgt tctttaagcc atggcgactg cgctttgcca aagagcaacg gttaccgctg
    31741 ttgttttacg gcgtttcgct gggtgggatg aattatcttt tttatctttc tattcagaca
    31801 gtaccgctgg gtattgcggt ggcgctggag ttcaccggac cactggcggt ggcgctgttc
    31861 tcttctcgtc gcccggtaga tttcgtctgg gttgtgctgg cggttcttgg tctgtggttc
    31921 ctgctaccgc tggggcaaga cgtttcccat gtcgatttaa ccggctgtgc gctggcactg
    31981 ggggccgggg cttgttgggc tatttacatt ttaagtgggc aacgcgcagg agcggaacat
    32041 ggccctgcga cggtggcaat tggttcgttg attgcagcgt taattttcgt gccaattgga
    32101 gcgcttcagg ctggtgaagc actctggcac tggtcggtta ttccattggg tctggctgtc
    32161 gctattctct cgaccgctct gccttattcg ctggaaatga ttgccctcac ccgtttgcca
    32221 acacggacat ttggtacgct gatgagcatg gaaccggcgc tggctgccgt ttccgggatg
    32281 attttcctcg gagaaacact gacacccata cagctgctgg cgctcggcgc tatcatcgcc
    32341 gcttcaatgg ggtctacgct gacagtacgc aaagagagca aaataaaaga attagacatt
    32401 aattaaattt acatttctgt atggttatgc ataaccatgc agaatttctc gctacttttc
    32461 ctctacaccg tctttatata tcgaattatg caaaagcata tttattccga aaattcctag
    32521 cgagcagata aataagaatt gttcttatca atatatctaa ctcattgaat ctttattagt
    32581 tttgtttttc acgcttgtta ccactattag tgtgatagga acagccagaa tagcggaaca
    32641 catagccggt gctatactta gtctcgttaa ttactgggac ataacatcaa gaggatatga
    32701 aattatgagt accgctaaat tagttaaatc aaaagcgacc aatctgcttt atacccgcaa
    32761 cgatgtctcc gacagcgaga aaaaagcaac agtagagttg ctgaatcgcc aggttatcca
    32821 gtttattgat ctttctttga ttaccaaaca agcgcactgg aacatgcgcg gcgctaactt
    32881 cattgccgta catgaaatgc tggatggctt ccgcaccgca ctgatcgatc atctggatac
    32941 catggcagaa cgtgccgtgc agctgggcgg tgtagctctg gggaccactc aagttatcaa
    33001 cagcaaaacc ccgctgaaaa gttacccgct ggacatccac aacgttcagg atcacctgaa
    33061 agaactggct gaccgttacg caatcgtcgc taatgacgta cgcaaagcga ttggcgaagc
    33121 gaaagatgac gacaccgcag atatcctgac cgccgcgtct cgcgacctgg ataaattcct
    33181 gtggtttatc gagtctaaca tcgaataaat ccatcgctga tggtgcagaa ctttagtacc
    33241 cgataaaagc ggcttcctga caggaggccg ttttgttttg cagcccacct caacgcactt
    33301 atttagtgca tccatctgct atccccagct gattaagtaa attttttgta tccacatcat
    33361 cacacaaccg ttacataaag attgtttttt catcaggttt tacgctaaat aatcactgtg
    33421 ttgagtgcac aattttagcg caccagattg gtgccccaga atggtgcatc ttcagggtat
    33481 tgctctataa atcgtgcatc acgtttttgc cgcatctcga aaaatcaatg agttgcaaaa
    33541 ctggcacgat tttttcatat atgtgaatgt cacgcagggg atcgtcccgt ggatagaaaa
    33601 aaggaaatgc tatgaagtct gtattaaaag tttcactggc tgcactgacc ctggcttttg
    33661 cggtttcttc tcatgccgcg gataaaaaat tagttgtcgc gacggatacc gccttcgttc
    33721 cgtttgaatt taaacagggc gataaatatg tgggctttga cgttgatctg tgggctgcca
    33781 tcgctaaaga gctgaagctg gattacgaac tgaagccgat ggatttcagt gggatcattc
    33841 cggcactgca aaccaaaaac gtcgatctgg cgctggcggg cattaccatc accgacgagc
    33901 gtaaaaaagc gatcgatttc tctgacggct actacaaaag cggcctgtta gtgatggtga
    33961 aagctaacaa taacgatgtg aaaagcgtga aagatctcga cgggaaagtg gttgctgtga
    34021 agagcggtac tggctccgtt gattacgcga aagcaaacat caaaactaaa gatctgcgtc
    34081 agttcccgaa catcgataac gcctatatgg aactgggcac caaccgcgca gacgccgttc
    34141 tgcacgatac gccaaacatt ctgtacttca tcaaaaccgc cggtaacggt cagttcaaag
    34201 cggtaggtga ctctctggaa gcgcagcaat acggtattgc gttcccgaaa ggtagcgacg
    34261 agctgcgtga caaagtcaac ggcgcgttga aaaccctgcg cgagaacgga acttacaacg
    34321 aagtctacaa aaaatggttc agtactgaac cgaaataata acgctacacc tgtaaagcgc
    34381 actggcagtt ccctctcccc cctggggaga ggattagggt gaggggcgca aacccgctcc
    34441 ggggccatta attaccctga atttgattat ttacaccacg gtaacaggaa caacatatgc
    34501 agtttgactg gagtgccatc tggcctgcca ttccgcttct gattgaaggt gccaaaatga
    34561 ccctgtggat ttcggtcctc ggtctggctg gcggtctggt aatcggattg ctggcaggtt
    34621 ttgcacgcac cttcggtggt tggatagcca accacgtcgc gctggtcttt attgaagtga
    34681 tccgcggcac acctatcgtc gtccaggtga tgtttatcta tttcgccctg ccgatggcgt
    34741 ttaacgactt acgcatcgac ccatttactg cggcggtggt caccatcatg atcaactccg
    34801 gcgcgtacat tgcggaaatc acgcgtggtg cggtgctgtc tatccacaaa ggttttcgtg
    34861 aagcaggact ggcgctcggt ctttcacgtt gggaaaccat tcgctacgtc attttaccgc
    34921 tggcactgcg tcgtatgctg ccgccgctgg gtaaccagtg gatcatcagc attaaagaca
    34981 cctcactgtt tattgttatc ggcgtggcgg aactgacccg tcaggggcaa gaaattattg
    35041 ccggtaactt ccgcgccctt gagatctgga gcgccgtggc ggtgttctat ctgattatta
    35101 ccctggtgct gagctttatt ctgcgtcgtc tggaaagaag gatgaaaatc ctgtgattga
    35161 atttaaaaac gtctccaagc actttggctc aacccaggtg ctgcacaata tcgatttgaa
    35221 cattgcccag ggcgaagtcg tggtgattat cgggccgtcc ggttccggta aatcgaccct
    35281 gctgcgctgc atcaacaaac tggaagaaat cacctccggc gatctgattg tcgatggcct
    35341 gaaggttaac gatccgaaag ttgacgagcg cctgattcgc caggaagcag gtatggtgtt
    35401 ccagcagttt tacctcttcc cgcatctgac ggcgctggga aacgtcatgt ttggtccgct
    35461 acgcgtgcgt ggcgcgaaca aagaagaggc ggaaaaactg gcgcgtgagc tgctggcgaa
    35521 agtcggtctg gcagaacgtg cacatcacta cccttccgaa ctttctggtg gtcaacagca
    35581 gcgtgtggcg attgcccgcg cgctggcggt gaagccgaaa atgatgctgt ttgatgaacc
    35641 gacttccgct cttgacccgg aactgcgcca tgaagtgctg aaggttatgc aggatctggc
    35701 tgaagaaggg atgacgatgg tgatcgtgac ccacgaaatc ggttttgccg agaaagtagc
    35761 ttcgcggctg atctttatcg acaaaggccg gattgcggaa gatggcaatc cgcaggtgtt
    35821 gatcaagaac ccgccgagcc agcgcttgca ggaatttttg cagcacgtct cttaataaga
    35881 cacattgcct gatgcgctac gcttatcagg cctacaggat atctggcaac ttattaaaat
    35941 tgcatgaact tgtaggacgg ataaggcgtt cacgccgcat ccggcaaaaa agcccgcacg
    36001 ttgtcagcaa ccggcttaat atcccttcct ccctttcacc cgaaagggag gcacaccaga
    36061 ttcctctcat ttaaaatcgc ccctccccca gcatctatac ttatcttttt gctctgtttt
    36121 ctcactggag gagtcatgcg gtggatcctg ttcatcctct tctgcctgct gggcgcacct
    36181 gcccacgcgg tatccatacc cggcgttaca accacaacga caacggactc aacgactgaa
    36241 ctggccccgg aaccggatat cgaacaaaaa aagcggccta tggcgcactg gcggatgtgc
    36301 tggataacga cacctcgcgt aaagagttga tcgaccagtt gcgtactgtc gccgcgacgc
    36361 cccctgcgga accggtcccg aagatcgtgc cgccgacgct ggttgaagag caaaccgtgc
    36421 tgcaaaaggt caccgaagtc agccgccatt atggtgaagc cctttccgcc cgcttcgggc
    36481 aactttatcg caatatcacc ggctccccgc ataagccatt taatccacaa accttcagca
    36541 atgcgctgag ccatttttca atgttagcgg tattagtgtt tggtttttac tggctgattc
    36601 gcctgtgcgc actgccgcta tatcgcaaaa tgggtcagtg ggcgaggcaa aaaaatcgtg
    36661 agcgcagcaa ctggttgcag cttccggcga tgattattgg ggcgtttatt atcgacctgc
    36721 tgttactggc cctgacatta tttgtcggcc aggtattaag cgacaacctg aatgcaggta
    36781 gtcgcaccat cgctttccaa caaagtttgt ttctcaacgc ctttgccctg attgaatttt
    36841 tcaaagccgt actacgcctg attttttgcc caaacgtggc ggagctgcgc ccgttcacga
    36901 ttcaggacga gagcgcccgt tactggagtc gccgcctgag ctggttaagc agcctgattg
    36961 gctatggcct gattgtggcc gtgccgatta tctctaatca ggtgaatgta cagataggtg
    37021 cgctggcgaa cgtcatcatt atgctgtgca tgaccgtctg ggcgttgtac ctgatcttcc
    37081 gaaataaaaa agagattacc cagcatttgc tcaactttgc ggagcattcg ctggcttttt
    37141 tcagcctgtt tatccgcgcc tttgcgctgg tgtggcactg gctggcaagc gcctatttta
    37201 tcgtgctgtt tttcttttcg ttgttcgatc cgggcaacag tctgaaattt atgatgggtg
    37261 caacggtgcg cagcctggcg attattggta tcgcagcgtt tgtttccggt atgttttccc
    37321 gctggctggc gaaaaccatc accctctcgc cacatactca gcgtaactat ccggagctac
    37381 aaaaacggct gaatggctgg ctttcggcgg cgctgaaaac ggcgcgtatt ctgaccgtct
    37441 gcgtggcagt aatgttgctg ttgagcgcat ggggattgtt cgatttctgg aactggctgc
    37501 aaaacggcgc ggggcagaaa accgtagata tcctgatccg catcgcactc attcttttct
    37561 tctcggcggt tggctggacg gtgctcgcca gtttgatcga aaaccggctg gcttcggata
    37621 ttcatggccg cccgctaccc agcgcccgta cgcgtaccct gctgacgctg ttccgtaacg
    37681 cgctggcggt gattatcagt accatcacca tcatgattgt gttgtcggaa atcggcgtca
    37741 atatcgcgcc attgctggca ggtgccgggg cattaggtct ggctatctcg tttggttcgc
    37801 aaacgctggt aaaagatatt atcaccgggg tatttattca gtttgaaaac ggcatgaaca
    37861 ctggagattt ggtgactatc gggccgttga ccggcacagt ggaacggatg tcgattcgct
    37921 ccgtgggcgt gcgccaggat accggggcgt atcacatcat tccgtggtct tcgataacca
    37981 cctttgctaa cttcgtccgc ggtattggtt cggtagtggc aaactacgat gttgatcgcc
    38041 atgaagatgc tgataaagcc aatcaggcac tgaaagatgc ggtaacggaa ttgatggaaa
    38101 acgaagaaat tcgcgggctg attattggtg aaccgaactt tgccgggatt gtcggcttaa
    38161 gcaataccgc gtttacactg cgtgtttcgt tcaccacgct gccactcaaa cagtggacgg
    38221 tacgctttgc cctcgacagc caggtgaaaa aacatttcga cctggcgggc gttcgcgcgc
    38281 cagtgcagac ttatcaggtg ctgcctgctc cgggcgcgac cccggctgaa ccgctgccgc
    38341 cgggggagcc aacgctttaa cgttggcgat taacaaaacg gcggcgctgc tcgtcgttca
    38401 taaaggtcca ggcaataaag cgactctgct tttgcccctg ggccatctct ttttttacca
    38461 ccttcaccgc gcccacgtcg gtcagagcac gatacaacgg cggtaagttt tcaccacgag
    38521 ataccagcga ggtaaaccac atcacctgct tcgcgaagcc tttgctctct tcaatcatct
    38581 ttttgataaa gttgacttca ccgccttcac accacaactc ctgttgctga ccaccaaagt
    38641 tcagtgcatc gtctttgttc agccccaggt tacggcgttt acgctcacta cctgcccgcg
    38701 ccgcagcggc ggaatcgtgg aatggcgggt tacacaaggt cgcgtcgtat tgctcgtttt
    38761 tgtggatgat gccgttaaaa atcgccccac tctctttttg ccgacgcaga cgaatggcgc
    38821 ggttaagccc cggattagca ctgataatcg cctgcgcact gcttaacgcc tggctgctgg
    38881 tttcactacc ggtaaaacgc cagccatatt catgtacgcc aatcagcgga taaatacagt
    38941 tcgcaccaac gccgatatcc agaatgctgg cattcgctgg aattgttccg cttgcttcag
    39001 ccagtaaatc ggcaaggtga tgaatataat ccgcccggcc cggtactggt gggcagagaa
    39061 aaccgtcggg gatatcccag ttcgctacgg cgtaaaaatg ggccagcaac gccttattga
    39121 gcgccttcac cgccagcgga ttggcaaagt ctacgctttg ctcccctgcg ggtgtaagcg
    39181 tgaggaattg cctgagttca ggattgacct gacaaagcgt ggcgagatcg tagcggctgt
    39241 gatgacggtt gcgcggatgc aaccccggtt tctgggcgga catagcattc tcctttaagc
    39301 agcggcgtaa gatacccgca taacgcgaca gggtaaatat ctggctgcgt ttatcattcc
    39361 ccaaaacctt aacatggcta tggtttatgg caattcatca taaaaatcag cattatgcat
    39421 gtcattgatt tgtattattt tgtaaaaata tatcgttcaa aaaataagca tatctctgtc
    39481 aattttttgc gtcatttgca tcgcgcaggc gacctacact tatcaggcac tacccgttgt
    39541 atttatgagg atgtcattat gaaaaagtgc ctcacactac tgattgccac cgtcctgagc
    39601 ggaatctctc tcacggctta tgccgcgcaa ccgatgagta acctggacag cggtcagcta
    39661 cggcccgccg gtaccgtttc ggcaactggc gcatcaaacc taagcgatct ggaggataaa
    39721 ctggcagaaa aagcgcgcga acaaggcgcg aaaggttatg tcattaattc cgcaggcgga
    39781 aatgaccaga tgttcggtac tgcaaccatc tacaaataac cccaccaacg taactgcatt
    39841 cgcctctgga tgcagttttc gcctgttttg taatcaaaaa acatcaaata tgaacgcaat
    39901 gtaaataaat gtatttcttt ttcgcgcaat gggtgataga aaatcgcgtc aagtgataat
    39961 acttatcaaa attattatca cttgcacaaa cactatcacg ggattaacag tggcatcgca
    40021 tccgcagaga tgctttctcg tggcagtgaa aatttcaaca tataagaaaa agtcacctgc
    40081 aaaatggaaa acaatcgcaa tttccctgcc agacaatttc attcgctcac gttctttgcc
    40141 ggtctttgta ttggtattac gcctgtggca caggcactcg ccgccgaagg gcaagctaac
    40201 gcggatgaca cgctggttgt cgaagcatca acgccttcgc tttatgcgcc acaacaatct
    40261 gccgatccga aattctcgcg tccggtagcg gatactaccc gcacgatgac ggtgatttct
    40321 gaacaagtga ttaaagatca gggcgcaacc aaccttaccg acgcgctcaa aaacgtcccc
    40381 ggcg
//
LOCUS       DAETPZ010000027        39558 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.027, whole genome shotgun sequence.
ACCESSION   DAETPZ010000027 DAETPZ010000000
VERSION     DAETPZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.027"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..190)
                     /locus_tag="K0W59_001651"
     CDS             complement(<1..190)
                     /locus_tag="K0W59_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HBI3022733.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKKG"
     gene            complement(331..2364)
                     /gene="metG"
                     /locus_tag="K0W59_001652"
     CDS             complement(331..2364)
                     /gene="metG"
                     /locus_tag="K0W59_001652"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBI3022734.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDKYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2496..3605
                     /gene="apbC"
                     /locus_tag="K0W59_001653"
     CDS             2496..3605
                     /gene="apbC"
                     /locus_tag="K0W59_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HBI3022735.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3867..4148
                     /locus_tag="K0W59_001654"
     CDS             3867..4148
                     /locus_tag="K0W59_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HBI3022736.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4441..4983
                     /locus_tag="K0W59_001655"
     CDS             4441..4983
                     /locus_tag="K0W59_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3022737.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            5064..5738
                     /locus_tag="K0W59_001656"
     CDS             5064..5738
                     /locus_tag="K0W59_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBI3022738.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            5754..8234
                     /locus_tag="K0W59_001657"
     CDS             5754..8234
                     /locus_tag="K0W59_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBI3022739.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPLVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            8250..9283
                     /locus_tag="K0W59_001658"
                     /pseudo
     CDS             8250..9283
                     /locus_tag="K0W59_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(9365..9703)
                     /gene="rcnB"
                     /locus_tag="K0W59_001659"
     CDS             complement(9365..9703)
                     /gene="rcnB"
                     /locus_tag="K0W59_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HBI3022740.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9922..10770)
                     /gene="rcnA"
                     /locus_tag="K0W59_001660"
     CDS             complement(9922..10770)
                     /gene="rcnA"
                     /locus_tag="K0W59_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HBI3022741.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTV
                     FWMFWRTWRGERNWLENMHGHDYEHHHHHHDHEHHQDHEHHHDQGHHHHHEHGEYQDA
                     HARAHANDIKRRFDGREVINWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLV
                     VSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHG
                     FMGIMR"
     gene            10891..11163
                     /gene="rcnR"
                     /locus_tag="K0W59_001661"
     CDS             10891..11163
                     /gene="rcnR"
                     /locus_tag="K0W59_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HBI3022742.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      11255..11351
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11386..12174
                     /gene="thiM"
                     /locus_tag="K0W59_001662"
     CDS             11386..12174
                     /gene="thiM"
                     /locus_tag="K0W59_001662"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBI3022743.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            12171..12971
                     /gene="thiD"
                     /locus_tag="K0W59_001663"
     CDS             12171..12971
                     /gene="thiD"
                     /locus_tag="K0W59_001663"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBI3022744.1"
                     /translation="MKRINALTIAGTDPSGGAGILADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            13036..13854
                     /locus_tag="K0W59_001664"
     CDS             13036..13854
                     /locus_tag="K0W59_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HBI3022745.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVDELQAFVDGIKETP"
     gene            13906..14652
                     /locus_tag="K0W59_001665"
     CDS             13906..14652
                     /locus_tag="K0W59_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBI3022746.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(14626..15591)
                     /locus_tag="K0W59_001666"
     CDS             complement(14626..15591)
                     /locus_tag="K0W59_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBI3022747.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIRAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15588..16591)
                     /locus_tag="K0W59_001667"
                     /pseudo
     CDS             complement(15588..16591)
                     /locus_tag="K0W59_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(16588..17064)
                     /locus_tag="K0W59_001668"
     CDS             complement(16588..17064)
                     /locus_tag="K0W59_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3022748.1"
                     /translation="MLARSSFHRKGIQKVLLCGIVIAYLTLPVNFTYALLFLGILLHG
                     VSYDFYYVTAYIYVDKKAPVHMRTAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQE
                     PVNGLTFNWSGMWTFGAVMIAIIAVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            17218..18270
                     /gene="fbaB"
                     /locus_tag="K0W59_001669"
     CDS             17218..18270
                     /gene="fbaB"
                     /locus_tag="K0W59_001669"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HBI3022749.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVAVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            18578..19432
                     /gene="gatY"
                     /locus_tag="K0W59_001670"
     CDS             18578..19432
                     /gene="gatY"
                     /locus_tag="K0W59_001670"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBI3022750.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRLKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNLAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            19461..20723
                     /gene="gatZ"
                     /locus_tag="K0W59_001671"
     CDS             19461..20723
                     /gene="gatZ"
                     /locus_tag="K0W59_001671"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HBI3022751.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAVVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            20733..21185
                     /gene="gatA"
                     /locus_tag="K0W59_001672"
     CDS             20733..21185
                     /gene="gatA"
                     /locus_tag="K0W59_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HBI3022752.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            21216..21500
                     /gene="gatB"
                     /locus_tag="K0W59_001673"
     CDS             21216..21500
                     /gene="gatB"
                     /locus_tag="K0W59_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HBI3022753.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQYRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            21504..22860
                     /locus_tag="K0W59_001674"
                     /pseudo
     CDS             21504..22860
                     /locus_tag="K0W59_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
     gene            22908..23948
                     /gene="gatD"
                     /locus_tag="K0W59_001675"
     CDS             22908..23948
                     /gene="gatD"
                     /locus_tag="K0W59_001675"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBI3022754.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLDPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            24054..24827
                     /locus_tag="K0W59_001676"
     CDS             24054..24827
                     /locus_tag="K0W59_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBI3022755.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQEAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(24909..25808)
                     /gene="yegS"
                     /locus_tag="K0W59_001677"
     CDS             complement(24909..25808)
                     /gene="yegS"
                     /locus_tag="K0W59_001677"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HBI3022756.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            26214..26531
                     /locus_tag="K0W59_001678"
     CDS             26214..26531
                     /locus_tag="K0W59_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022757.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(26873..28234)
                     /gene="yegQ"
                     /locus_tag="K0W59_001679"
     CDS             complement(26873..28234)
                     /gene="yegQ"
                     /locus_tag="K0W59_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HBI3022758.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(28381..28703)
                     /locus_tag="K0W59_001680"
                     /pseudo
     CDS             complement(28381..28703)
                     /locus_tag="K0W59_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
     gene            complement(28894..29616)
                     /gene="baeR"
                     /locus_tag="K0W59_001681"
     CDS             complement(28894..29616)
                     /gene="baeR"
                     /locus_tag="K0W59_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HBI3022759.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHESGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(29613..31016)
                     /gene="baeS"
                     /locus_tag="K0W59_001682"
     CDS             complement(29613..31016)
                     /gene="baeS"
                     /locus_tag="K0W59_001682"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HBI3022760.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLIDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(31013..32428)
                     /locus_tag="K0W59_001683"
     CDS             complement(31013..32428)
                     /locus_tag="K0W59_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3022761.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(32429..35494)
                     /gene="mdtC"
                     /locus_tag="K0W59_001684"
                     /pseudo
     CDS             complement(32429..35494)
                     /gene="mdtC"
                     /locus_tag="K0W59_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
     gene            complement(35495..38617)
                     /gene="mdtB"
                     /locus_tag="K0W59_001685"
     CDS             complement(35495..38617)
                     /gene="mdtB"
                     /locus_tag="K0W59_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HBI3022762.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPSASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLNVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLVLWTK
                     SRFARHEEEA"
     gene            complement(38617..>39558)
                     /locus_tag="K0W59_001686"
     CDS             complement(38617..>39558)
                     /locus_tag="K0W59_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HBI3022763.1"
                     /translation="TGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDP
                     SQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETEGT
                     IKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDL
                     VFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKA
                     RFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKHLV
                     TPGIQDSQKVEVVEAQSATTPEEKATSREYAKKGARS"
BASE COUNT         9919 a         9685 c        10238 g         9716 t
ORIGIN      
        1 accccttctt caccttctcc gcaatcagct tcagttccgc tttttccgcc gccgcagcat
       61 ctgaaaaact ttttatctga ctttgccctt tggtgccaac ttttccccaa ctgatatgca
      121 actcgtttcc ctgttgctcc accgtccaga atttatgtga tttttcgtcc tgatagataa
      181 agtgtctcat tctgcattcc tgtaatttta tccagttgaa tgcagatgct accagtattt
      241 atacgggtta gagagagaca aaatcgcaag aaaacaactt taggaattta taaagaaaag
      301 gcgctgtcga tgcagcgcct tgaaggggga ttatttcacc tgatgacccg gtttagcacc
      361 ggcatccggg cttagcagga aaatatcttt cccgccagga ccggcagcca tcaccatgcc
      421 ttcagagata ccgaagcgca ttttacgtgg tgccaggtta gccaccataa tggtgtgacg
      481 accaatcagt gcctgcggat ccgggtaagc agaacgaata ccggagaaga cattgcgttt
      541 ttcaccgccg agatccagcg tcaggcgcag cagtttgtca gaaccttcaa caaactctgc
      601 gttttcgatc agcgccacgc gcaggtcaac tttggcgaag tcgtcaaagg tgatggtttc
      661 ctgaatcgga tcatctgcca gtgggccagt aactggcgcg gtagtggctt tcacttcttc
      721 tttagaggct tccaccagag cttcaacctg tttcatatcg atgcggttat acagcgcctt
      781 gaacggattc actttgtggc ccagcagcgg ttgctggata ccatcccagg tcagttccgt
      841 attgaggaag gcttctgcac gctcggtcag tttcggcagt accggcttca ggtaagtcat
      901 cagcacgcgg aacaggttga tgcccattga gcaaatcgcc tgcaggtcag catcgcgacc
      961 ttcctgtttt gcgactaccc acggagcctg ctcgtcgaca tagcggttag ccaggtcagc
     1021 cagtgccatg atttcgcgca ctgctttacc aaattcacgg ctttcccacg cttcaccaat
     1081 cacttcagcg gcatcggtaa aggttttgta cagctcaggg tcagccagct cgcttgccag
     1141 cacaccgtca aaacgcttgt tgataaagcc cgcattacgg gaggccaggt taaccacttt
     1201 gttcacgata tcggcattca cacgctgaac gaaatcttcc aggttgagat cgatatcatc
     1261 aatgcgcgaa gagagtttcg cagtgtagta gtaacgcagg ctgtcagcgt caaaatgatt
     1321 cagccaggtg ctggctttaa taaaggtgcc gcgagacttg gacatcttcg cgccgttcac
     1381 cgtcacatag ccatgaacaa acaggttggt cggcttgcgg aagttgctgc cttccagcat
     1441 ggcaggccag aacaggctgt ggaagtaaac gatatcttta ccgatgaagt ggtacagctc
     1501 ggcggtggag tctttcttcc agtatttatc gaagcttacg ctgtcgccgc gcttgtcgca
     1561 cagattcttg aaagaaccca tgtagccaat cggtgcgtcc agccagacgt agaaatattt
     1621 gcccggcgcg ttcggaattt caaaaccaaa gtatggcgcg tcgcgggaga tatcccactg
     1681 ttgcaggccg gattcaaacc actcctgcat tttgtttgcc acctgctcct gcaacgcacc
     1741 gctgcgggtc catgcctgca gcatttcgct gaaagagggc agatcaaaga agaagtgttc
     1801 agaatcacgc atcactggtg tagcaccaga aaccaccgat ttcggctcga tcaattcagt
     1861 cgggctgtag gtcgcgccgc agacttcgca gttatcgccg tattgatccg gggatttaca
     1921 tttcgggcag gtgcctttca caaaacggtc cggcaggaac atgccttttt ccggatcgta
     1981 cagctgagag atggtgcggt ttttaataaa accgttttct ttcaggcgag agtagataag
     2041 ttctgacaac tggcggttct cttcgctgtg cgtcgagtga tagttgtcat agctgatgtt
     2101 aaagcctgcg aaatcagtct gatgctcctg actcatttcg ccaatcatct gctccggggt
     2161 gataccgagc tgctgagctt tcagcatgat cggtgtaccg tgggcatcgt cggcgcagat
     2221 gaagttgacc tcgtggccgc gcattcgctg gtaacggacc cagacatcag cctggatgtg
     2281 ctccagcata tggccgaggt ggattgagcc gttagcgtac ggcagtgcgc acgtcaccag
     2341 aattttcttc gcgacttgag tcatagtagg cattacttct taatagtgaa aaggggttag
     2401 atagtaccaa atgggaaaat gttaagtaag tacttctcta tattcttcag gtaaactatc
     2461 agctgatggt ttatctatac aaaaaaggag tcgggatgaa cgaacaatcc caggccaaat
     2521 caccagaagc cttgcgcgca atggtcgccg ggacactggc caattttcag cacccaaccc
     2581 tgaagcataa cctcaccacg ctgaaagcgt tgcaccatgt cgcctggatg gacgacacgt
     2641 tgcatgtgga actggttatg ccgtttgtct ggcatagcgc gttcgaagag ttaaaagagc
     2701 aatgtagcgc cgaactgctg cgcatcaccg gggcgaaagc tatcgactgg aaactgtcgc
     2761 ataacatagc cactctgaaa cgcgtgaaga atcagccggg aattaacggt gtgaagaaca
     2821 ttatcgcggt gagttccggt aaaggcggcg tgggtaaatc gtccacggcg gtaaacctgg
     2881 cgctggcact ggcggcggaa ggggcgaaag tcggcattct ggacgccgat atctacggcc
     2941 catcaattcc taccatgctg ggcgcagaaa atcagcgtcc aacttcgccg gacggtaccc
     3001 acatggcacc gattatgtct catggcctgg caaccaattc aattggttat ctggtcactg
     3061 acgacaatgc gatggtgtgg cgtggaccga tggcgagcaa ggcgttaatg cagatgttgc
     3121 aggaaacctt gtggccggat ctcgactatc tggtgcttga tatgccgcca ggcaccggtg
     3181 acattcaatt gacgctggcg caaaacattc cagtaactgg tgcagtcgtg gtcaccaccc
     3241 cgcaggacat cgcgttgatc gatgcgaaaa aaggcattgt gatgttcgag aaagtcgagg
     3301 ttccggtgct cggtattgtc gagaacatga gtgtgcatat ttgcagtaac tgtggtcacc
     3361 atgaaccgat tttcggtacc ggtggtgcag agaaactggc tgaaaaatac catacacagc
     3421 tgctggggca gatgccgtta cacatctcct tacgtgagga tctcgataaa ggcacgccaa
     3481 cggttatcag ccgtccggag agcgaattta ccgctatcta ccgccagctg gctgaccgcg
     3541 ttgcagctca gctctactgg cagggtgaag tcattcctgg cgagatttcc ttccgcgcgg
     3601 tctaacgcct attaaccatt ctggttattc ttcatacacc gcagagatgt tatattgatg
     3661 cggtgtattt gtttttgccg cattaatgaa cacttattta atactgcgaa tattctgcgg
     3721 accttcgtcc gaaactctac aaagtcgagt gatcgtacag gcaatgcttc attatcatcc
     3781 ttcgtgctgt gtagtggatg tacagctata gtttgttatg cactcattct atccaggcat
     3841 aacctttact acagggataa cgttcaatga ataagtattg gctgtcagga attatttttc
     3901 ttgcctatgg attggcttct cccgcagtct cttcagagac tgccacatta acgattaatg
     3961 gcaggatcag tcccccaaca tgcagtatgg ctatggttaa tagtcaacca caacagcatt
     4021 gtggtcagtt aacgtataat gttgacaccc gtcatcaggt ttcttcgccc gtcaaaggtg
     4081 ttacgacaga agtggttgtt gcaggtagcg atagcaaacg tcgaatcgtg ttaaatcgct
     4141 acgattaatt tggcaacctg attttaattg catttagaaa gacgaaatca tttatataaa
     4201 tgatttcgtc ttttttattg atatcaacaa taccatttac ataaatctac attaattaat
     4261 agatgtgata aatatttaat attcttaagt atttgttgta tttatattgc attggttaat
     4321 ttgttttaaa tcaataaaac aatatgttta cattgcggca gcttttttga tgtgagaaat
     4381 ttgtttccaa gagatacaga ctcttaacaa ccaaaaataa attttattaa ggaacatatt
     4441 atgaaacgtt caattattgc tgccgctgtc ttttcttctt tttttatgag cgctggagta
     4501 tttgctgcag acgttgatac cggaacatta accattaaag gaaatattgc agaatctccg
     4561 tgtaaattcg aagcgggtgg tgattcagta agtattaata tgccgactgt accaaccact
     4621 gtctttgaag gtaaagctaa atattctacc tatgatgatg cagtcggtgt aaccagcagc
     4681 atgttaaaaa ttagctgccc gaaagaagtt gctggtgtaa aactctcgtt gatcacaaac
     4741 gacaaaataa ccggtaacga taaggcgata gccagtagca acgataccgt gggttactat
     4801 ctctatttag gtgataacag cgatgtccta gatgtttctg caccttttaa cattgagagt
     4861 tataaaacag cagaaggtca atatgctatt ccgtttaaag caaaatacct gaaactgaca
     4921 gataactcag tgcaatcagg tgatgtgtta tcttctctgg ttatgcgtgt agcgcaggat
     4981 taataaataa aattgcaagt ttctccaggt aataaacatg gccgctatcc ccatggcggc
     5041 tttttaattt aagaggcatt aagatgaaag gattattatc tttactcatt ttttctatgg
     5101 tccttcctgc acatgccgga attgttatct acgggacgcg cattatttac ccggcagaaa
     5161 ataaagaagt tatggtgcag ttgatgaacc agggaaatcg ttcttcgctg ctgcaggcgt
     5221 ggattgatga tggcgatacg tcgttaccac cagaaaaaat tcaggttcct ttcatgttaa
     5281 cgccaccagt ggcaaaaata ggggcaaatt ccgggcagca agtaaaaatc aaaattatgc
     5341 cgaataaact gcccactaat aaagaaagca ttttttatct gaatgttctg gatattccac
     5401 caaatagtcc agagcaagaa ggcaagaatg cactgaagtt tgcgatgcaa aacagaatta
     5461 agttgtttta ccggccagcg ggtattgctc cggtaaataa agcgaccttt aaaaagttac
     5521 tggtaaatcg cagtggcaat ggattggtga taaaaaatga ctcagctaat tgggtgacga
     5581 tttcggatgt caaagctaat aatgttaaag tcaattatga aactattatg attgccccct
     5641 tagaaagtca gagtgttaat atcaaaagta ataatgcaaa taactggtat ctgaccatta
     5701 tcaatgacca cggcaactat attagtgaca aaatttaatt gcaggagctg cctatgttga
     5761 gaatgacccc acttgcatca gcaatcgtag cgttattgct cggcattgaa gcttatgcag
     5821 ctgaagaaac ctttgatacc cattttatga taggtggaat gaaagaccag caggttgcaa
     5881 atattcgtct tgatgataat caacccttac cggggcagta tgacatcgat atttatgtca
     5941 ataagcaatg gcgcgggaaa tatgagatta ttgttaaaga caacccgcaa gaaacatgtt
     6001 tatcaagaga agttatcaag cggttaggca ttaatagcga taacttcgcc agcggtaagc
     6061 aatgtttaac atttgagcaa cttgttcagg gtgggagcta tagctgggat atcggggttt
     6121 ttcgtctcga tttcagtgtc ccgcaggctt gggtggaaga actggaaagt ggctatgttc
     6181 caccggaaaa ctgggagcgg ggtattaatg cgttttatac ttcttattat gtgagtcagt
     6241 attacagcga ctataaagcg tcgggtaaca acaagagtac atatgtacgt tttaacagcg
     6301 ggttaaattt actggagtgg caactgcatt ctgatgccag tttcagtaaa acaaataaca
     6361 atccaggggt gtggaaaagc aataccctgt atctggaacg tggatttgcc caatttctcg
     6421 gcacgcttcg cgtgggtgat atgtacacat caagcgatat ttttgattct gttcgcttca
     6481 gcggtgtgcg gttgtttcgt gatatgcaga tgttgcctaa ctcgaaacaa aattttacgc
     6541 cacgggtgca ggggattgct cagagtaacg cgctggtaac tattgaacag aatggttttg
     6601 tggtttatca gaaagaggtt cctcctggcc cgtttgcgat tacagatttg cagttggccg
     6661 gtggtggagc agatcttgat gttagcgtga aagaggcgga cggctcggta accacctatc
     6721 tggtgcctta tgcagcggtg ccaaatatgc tgcaacccgg cgtgtcgaaa tatgattttg
     6781 cggcgggccg tagccatatt gaaggggcga gcaagcaaag tgattttgtc caggcaggtt
     6841 atcagtatgg ttttaataac ttattgacgc tgtatggcgg cacgatggtt gctaataatt
     6901 actatgcgtt taccctcgga acgggttgga acacgcgcat tggcgcaatt tccgtcgatg
     6961 ccacgaagtc gcatagtaaa caagacaacg gcgatgtgtt tgacgggcaa agttatcaaa
     7021 ttgcctacaa caaatttgtg agccaaacgt cgacgcgttt tggtctggcg gcctggcgtt
     7081 attcgtcgcg tgattaccgg acatttaacg atcacgtttg ggcaaacaat aaagataatt
     7141 atcgccgtga tgaaaacgat atctatgaca ttgccgatta ttaccagaac gattttggcc
     7201 gtaaaaatag cttttctgcc aatatgagcc agtcattgcc agaaggctgg ggttctgtgt
     7261 ccttaagtac gttatggcga gattactggg ggcgtagcgg cagcagtaag gattatcagt
     7321 tgagttattc caataactgg cggcggataa gctataccct cgcggcaagc caggcgtatg
     7381 gcgagaatca tcatgaagag aaacgtttta atatttttat atcaattccc tgtgattggg
     7441 gtgatgacgt tacgacgcct cgtcggcaaa tatatatgtc taactcaacg acgtttgatg
     7501 atcagggttt tgcctcaaat aatacgggat tatcaggaac agtagggagt cgggatcagt
     7561 tcaattatgg tgtcaacctg agtcatcaac atcagggaaa tgaaacgaca gctggggcga
     7621 atttgacctg gaacgcgccg gttgcgacag tgaatggcag ttatagtcag tcgagtactt
     7681 atcgacagac tggagccagt gtttcagggg gcattgtcgc ctggtcgggt ggcgttaatc
     7741 tggcgaaccg tctttccgaa acgtttgctg tgatgaatgc gccaggaatt aaagatgctt
     7801 atgtcaatgg gcaaaaatat cgcacaacaa accgtaatgg agtggtggta tacgacggaa
     7861 tgacacctta tcgggaaaat cacctgatgc tggatgtgtc gcaaagcgat agcgaagcag
     7921 aattacgtgg caaccggaaa attgccgccc cttatcgcgg cgcggttgta ctggttaatt
     7981 ttgataccga tcagcgcaag ccctggttta taaaagcgtt aagagcggat ggtcaaccat
     8041 taacgtttgg ttatgaagtc aatgatatcc atggtcataa tattggcgtt gtcggccagg
     8101 gaagccagtt atttattcgc accaatgaaa taccgccatt ggttaatgta gcaattgata
     8161 agcaacaagg actttcatgc acaatcacct tcggtaaaga gattgatgaa agtagaaatt
     8221 atatttgcca gtaatagtca ggtggttcta tggaaattcg cataatgcta tttatattaa
     8281 tgatgatggt tatgcctgtg agctatgcgg catgttatag tgagttatct gttcagcaca
     8341 atttggttgt tcaggggaat tttgcactta ctcaaacaca aatagcgaca tatgagcata
     8401 attttaatga ttcgtcatgc gtgagtacaa atactatcac ccctatgagc ccatcggata
     8461 ttattgttgg actttataac gataccatta aattaaattt acattttgaa tggaccaata
     8521 aaaacaacat cacgttgtca aataatcaga gcagtttcac cagtggttat tcggtaacgg
     8581 tgacacctgc ggccagtaat gcaaaagtga atatttctgc ggggggcggt ggttcagtga
     8641 tgattaatgg tgttgcgaca ttatccagtg cttcatcatc gacacgcggg agtgccgcag
     8701 tacaatttct actgtgttta ttaggtggca agtcatggga tgcatgtgta aatagctaca
     8761 gaaatgcatt ggcacaaaat gcaggtgtct attccttcaa tctgacattg tcatacaacc
     8821 cgataaccac aacctgcaaa caggacgatt tattaattac tttagacagt attcccgttt
     8881 cacaattacc agccacaggt aacaaagcaa caataaatag taaaaaaagg gatattattc
     8941 tgcgttgtaa aaatttatta ggtcaacaaa atcaaacatc acggaaaatg caggtgtatt
     9001 tatcaagttc tgacttgtta accaacagcg acacaatact gaaaggtgcg gaagataatg
     9061 cgtaggattt attcttgaaa gtaatggttc gccagtcaca cttttaaata tcactaacag
     9121 cagtaaagga tacacaaatt taaaagaaat tgcggcaaag tcaaaactta cagatacaac
     9181 ggtttcaatt ccgataacag ccagttacta cgtctacgat acaaacaaag ttaaatctgg
     9241 cgcactggag gcaaccgcat taatcaacgt gaaatacgac taataacaag caagacgagc
     9301 aagtggctaa taataaaaaa taacaaggtg tctgcatttc cccctcatga tgagggggct
     9361 ttttttagcg atgataaaaa atctcaccgt cgtaggcttt aatgatttta ccgtcggtgt
     9421 cgctgatcaa cacgtacgcg ccacccatat aggtccagtg cgtcccggca tcaggcgcgg
     9481 gcaggttacg taactgccac tgcttaatgt tgtactcatc ggtgcggtac atctctggga
     9541 cggtatcacc tggtttgaaa tgcttaaagt cagcaatgaa actacttaac tcatacttag
     9601 aaattcccga ggtattggtc gaacccgcgg tggctggtgc ggcccaggcg gcactggtaa
     9661 ccagcaaaag cacacccagc aacatcttat ttttaatggt cataaactct ccactttggg
     9721 gatctgtaat atcagtcgca tatctttcct gccatacaat ataactgaca agcgatattt
     9781 tgttagtgat cccatgattt cgtaaaaagt gtaaatgaaa ggatgcccta aaagcatcct
     9841 tagcgagcag agagtcagcg gaaatagctg atttaagaat catcctgggc tgaagataac
     9901 atgcaagcct aaaggattga gttatcgcat tatgcccatg aagccatgta cgcccatata
     9961 cacaccgact aagccaatca acagactgga aaaatagggg gcgcgtttag cgagagtgtt
    10021 aaatccgctc cagcgttttg cgacctgctg aacgctgatt gctgcgccaa cgcctacggt
    10081 gacaagcgtt aacgccaggc caatgctgaa actgacgacc agtgttgcgc ccagtgtcag
    10141 ggctttcaac tgaatgcata tcaacagcac ggtaattgct gccgggcagg ggataaggcc
    10201 accggttaag ccaaataaca aaatttgcca gttgatgacc tctctaccat caaagcgtcg
    10261 tttaatgtca ttggcatggg ctcgtgcatg ggcatcctga tactcgccat gttcgtgatg
    10321 gtgatgatgt ccttggtcgt ggtgatgttc gtggtcttga tgatgttcgt gatcgtgatg
    10381 atggtgatga tgctcataat catgcccgtg catattctcc agccagttgc gttcgccgcg
    10441 ccaggtacgc cagaacatcc agaacacggt gccaataatg atcactgcgg aaatcagctg
    10501 gagccacggt tctgctgatt gagcagtaaa gcgcttactg atcaccatcc cgccaaaggc
    10561 aatcaaccag accaccgcgg tatgcgaaat ggttgctgcc agtccgagca tcaccgcctg
    10621 tttaatggtg cctttgatgg cgatgataaa cgccgccatc atcgtttttg agtgccctgg
    10681 ttccaggcca tgaagcgcac caagtaagat ggcgctgggg atgaagaacc aggcgtttcc
    10741 ttgctgaaga agagttgtaa attcggtcat gataataatt cttagtatta attcggcaat
    10801 ctgattctac tcccccccag tacctgatac taccccccag tagattaata gtgctatgat
    10861 ttttcatatt cttgttaacc aggtgttgcc atgtctcata caatccgtga taaacagaaa
    10921 ctgaaagcgc gtgccagtaa gattcagggc caggtcgtgg cgctcaagaa aatgctcgac
    10981 gagccgcacg aatgcgctgc agttttacaa cagattgctg ctatccgtgg agcggtaaac
    11041 ggtctgatgc gggaagtgat taaaggtcat ctgacggaac acatcgttca ccagggggat
    11101 gagctaaaac gtgaagaaga tctggatgtc gttctgaagg tgctggattc atatatcaaa
    11161 taatttatta acgcgattgt aaaactgccg tttttcctcg tttacaacgg gtgcgctgga
    11221 cattaccatc ctcctctgcg atttatcatc gcaaccaaac gactcggggt gcccttctgc
    11281 gtgaaggctg agaaataccc gtatcacctg atctggataa tgccagcgta gggaagtcac
    11341 ggaccaccag gtcattgctt cttcacgtta tggcaggagc aaactatgca agtcgacctg
    11401 ctgagttcag cgcaatctgc gcacgcgtta cacctttttc accaacattc ccctcttgtg
    11461 cactgcatga ccaatgatgt ggtgcaaacc tttaccgcca ataccttgct ggcgctcggt
    11521 gcatcgccag cgatggttat cgaaaccgaa gaggccagtc agtttgcggc tatcgccagt
    11581 gccttgctga ttaacgttgg cacactgacg cagccacgcg cacaggcgat gcgtgctgcc
    11641 gttgagcaag caaaaagctc tcaaacacgc tggacgcttg atccagtagc ggttggtgcg
    11701 ctcgattatc gccgccattt ttgtcatgaa cttttatctt ttaaaccggc agcgatacgt
    11761 ggtaatgctt cggaaatcat ggcattagct ggcattgcta atggcggacg gggagtggat
    11821 accactgacg ccgcagctaa cgcgataccc gctgcacaaa cactggcacg ggaaactggc
    11881 gcaatcgtcg tggtcactgg cgaggtggat tatgttaccg atggacatcg tatcattggt
    11941 attcacggtg gcgatccgtt aatgaccaaa gtggtaggaa ctggctgtgc attatcggcg
    12001 gttgtcgctg cctgctgtgc gttaccaggc gatacgctgg aaaatgtcgc atctgcctgt
    12061 cactggatga aacaagccgg agaacgcgca gtcgccagaa gcgaggggcc aggcagtttt
    12121 gttccacatt tccttgatgc gctctggcaa ttgacgcagg aggtgcaggc atgaaacgaa
    12181 ttaacgctct gacgattgcc ggtactgatc cgagtggtgg tgcggggatt ctggccgatc
    12241 ttaaaacctt ctcggcactt ggcgcttatg gttgctcagt tattactgca ctggtggcgc
    12301 aaaatacccg tggcgtacag tcggtgtatc gcattgagcc tgattttgtc gccgcccagc
    12361 tcgattcggt gttcagcgat gtgcgaatcg ataccactaa aatcggtatg ttggcggaaa
    12421 ccgatattgt tgaagcggtg gcagaacggt tgcaacgtta tcagatccaa aacgtggtac
    12481 tcgacaccgt tatgctggca aaaagcggcg acccgctgct ttcaccttcg gcggttgcta
    12541 cgctgcgcag tcgattattg ccacaggttt cattaataac gccaaacttg cccgaagctg
    12601 ccgccttgct cgacgcgcca cacgcgcgca ccgaacagga aatgctggaa caagggcgat
    12661 cgctgttggc gatgggctgt ggcgcagtgc taatgaaagg tggtcatctg gatgatgagc
    12721 aaagcccgga ctggctgttt acccgcgagg gtgaacaacg gtttaccgca ccgcgcatta
    12781 tgaccaaaaa cacccacggc actggttgta cactctctgc ggcgttggct gcactacgcc
    12841 cgcgccatac aaactgggct gacaccgtac aggaggcaaa aagctggctt tcatcggcgt
    12901 tagcccaggc cgacacgctg gaagttggtc acggtattgg tccggttcac cacttccacg
    12961 cctggtggtg agcaccgtac tatagctgct ttggttattg tcagcctttg gcactgacat
    13021 tcagggatga aaccaatgca gctaagaatt accagtcgaa aaaaactcac cgctctatta
    13081 tgcgctcttg ggctaatttc catcgttgca atttatccgc gtcagacggt gaattttttc
    13141 tattcgacag cagttcagat tacagactac atccacttct acggttatcg cccggttaaa
    13201 tctttcgcta ttcgcattcc tgccagttac accattcacg gattagatgt ttcgcgctgg
    13261 caggagcgta tcgactggca gcgggtggca aaaatgcgcg acaacgatat ccgcttacag
    13321 tttgctttta ttaaggctac ggaaggcgaa aagctggtgg acccctattt ttcgcgtaac
    13381 tggcaattaa gccgcgaaaa tggcctgctg cgcggggcgt atcattattt ctccccgtcg
    13441 gtatccgctt cagttcaggc gcgattattt ctgcaaacgg tggatttctc acaaggcgat
    13501 ttccctgccg tgctggacgt agaagaacgg ggaaaattat cggcaaaaga attacgcaag
    13561 cgggtaagtc agtggctaaa aatggtcgaa aaacgcacgg gaaaaaagcc gattatttac
    13621 tcaggagccg ttttttatca caccaatctg gcgggctatt tcaatgaata tccgtggtgg
    13681 gtggctcact attatcaacg tcgtccggac aatgacggca tggcctggcg cttctggcag
    13741 cattccgacc gtggacaggt agatggcatc aatggtccgg tggattttaa tgtgtttaat
    13801 ggcacggtgg atgagttgca ggcattcgtt gatgggatta aagaaacgcc ttaaataaga
    13861 agcagtgata tactcccacc agactccctg gcaggaaaaa gacaaatgga acaagcgcat
    13921 acccagctga ttgcccaact gaacgagcgt attttagcgg cggataacac gccgctttat
    13981 attaagtttg ccgaaacggt aaaaaatgcc gtgcgcagcg gggtgctgga gcatggcaat
    14041 attttgcccg gtgagcgtga tttaagtcag ttaaccggcg tttcgcgcat tacggtgcgc
    14101 aaggcgatgc aggcgctgga agaagagggc gtggtgacgc gttcgcgcgg ttacggcacg
    14161 cagatcaaca acatcttcga atactcgttg aaagaagcga gaggtttttc tcagcaggtg
    14221 gtattgcgcg ggaaaaagcc cgatacgcta tgggttaata agcgtgtcgt gaaatgtccc
    14281 gaagaagttg cgcagcagct ggcggtcgaa gcgggaagtg atgtcttttt gcttaagcgt
    14341 attcgctatg tcgatgaaga agcggtatcg attgaggaat cgtgggttcc ggcgcattta
    14401 attcatgatg ttgatgccat cggaatttct ctttatgact atttccgcag ccagcacatt
    14461 tacccacaac gtacgcgttc ccgcgttagc gcccggatgc cggatgccga gtttcagtca
    14521 catattcagt tagatagcaa aataccggtg ctggtgatca agcaagtggc gcttgaccaa
    14581 cagcaacggc ctattgagta cagcattagc cactgtcgca gcgatctata cgtttttgtg
    14641 tgcgaggagt agttcctcgc gcgttggcgc acaatcaccg ccccgatgcc cgacaaccca
    14701 cgacgccact gcattgccca gtaatacggc atccgccagt ggcagaccag aggccagccc
    14761 ggcaagtacg ccacctgcat gactgtcgcc cgccccaatg gtgtctacaa cttgcgttgg
    14821 aaatgcccga atgcagccag aagcgtcgtt gctgaaatac catgcgcctt ctttatcgag
    14881 acgaacgatc aacggcgcgg caaatttctc ctgccattgc ttgccaagtg ttgttatctc
    14941 tgcagataaa gcaaaacgtt cggcggcaat ctcagcctct tgacgattga gtgacactaa
    15001 aggtcgacag gccatgatcc gcgccagtaa tgcgtccggg atatcgccaa tacgtgggcc
    15061 aaaatcgata aacggcgtca cgtcttgcag cttttccagc cattccacta acaattcgcc
    15121 gcagggcgag gccagttgat aaccggaaaa atagagcagg ttgccaggcg caacggttaa
    15181 tcgtgccaac cattggcgat tccattgatt ttccacaccg ctgaatgaca taaaagtgcg
    15241 ttcgccatcc ggctcaacca gtgccagaca ccagccgtta tcgctttcgg cgttatcgat
    15301 caggctgatt aacccctctt tcgccatccg gttgcgaata atctccgccc acacgccctg
    15361 accgagcggc aaggcattac ccgcttcgat gccgaggcgc tttaacgcca cggcaatatt
    15421 cagtgcgcag ccgccaacgt taacgctctg ctgtttcagt tcgatatcgc acccacgcca
    15481 ggggagggca taagcgtcgg cgatcacgtc aatgaccgcc gcgccgacga ccatcaccgg
    15541 ctgacgcgtg gttaattcag gcagcagtgt gtgtaatcga gcgccgctca taccgcctcc
    15601 cgttgttgac gatatttcgc cagcgctgtg gcatagcggt tgaaatcaag ctgatttacc
    15661 gcatccagct ccgcctttaa cgcaggatcg acagcgttaa tgccatgcaa cgcgccgcaa
    15721 atcgccgtcg ccatagcacc tatggtgtct gtgtcgccgc caagattggc gcacaggacg
    15781 gcgcagcggt ttgggtcggt ttgtgccagt tcaactagcg caatggcgca cggaacggac
    15841 tcaatagtgc tggtgcctgc gccaacgacc tggtaaagct gttcgctggc ggattcggtg
    15901 ccgtcggcat tacgcacaat tttcagcgca atctccagac gtgccgccag tgaggcgctg
    15961 aacgtggtgg tgcgtttttg ctgtgcatgt cgcgcaattg aaggcaatga atcaacaatc
    16021 gctgaccagc gttctccgtc aatggcacga gaaatcgccc atgcgatgac taccgcgcct
    16081 gcaaccgcca gatcggattt atgggtcggg ctggacgcca gcgccacatc atcaataaag
    16141 gaatcaacat cacgcgctgg aagcaaacaa cctaatggcg agacgcgcat cgctgcgccg
    16201 ttggttacgc cgttgttttc cagttcagca acgggtttac cgtcgcgaat ggcgttaagc
    16261 gcaatcttcg aggtcggacc tagtacgttt ttgttaaagg cgtcgaaacg caacgcccag
    16321 tcgagaatat tacgcccaat cagattcgga tcgatcttgc cttcacattc cagtaacgca
    16381 tccgccagac acaggccatc gaggtatcgt cggtgaattc ggcgcggtta aaataacagg
    16441 ccgcgttatt ctcctttggt ccaggcagaa aacggtcaat ccagccaaag tgtgctttaa
    16501 cgcggctgcg tggccaaagc tcggagggca tccccatcgc atcccctaac gcctgcccat
    16561 aaagagcacc gagaatacgt tctgttttca tttaacttcc ccttgtgtca acgcaatatc
    16621 tcgatcatcg accttgatag ccgtaatttc gttgtcggat tcgcggaaaa aaatcatgaa
    16681 cagcacggcg ataatggcaa tcatcaccgc gccgaaagtc cacatcccgg accagttgaa
    16741 agtcagtccg tttaccggtt cctgataagc gaacatcttt tccatcatca caccgccaag
    16801 acgatagccg agcaaactgc cgaagccctg gcagcagagc gtgatcagcc cctgcgcagc
    16861 ggtacgcata tgcacggggg cttttttatc gacatagatg taagcggtaa cgtagtaaaa
    16921 atcgtaactt acgccgtgaa gcaaaatacc gaggaacagt aacgcgtagg tgaaattgac
    16981 aggtaatgta aggtacgcga tgacaatacc acataataat accttttgaa tacctttgcg
    17041 gtgaaaactt gaccgcgcta acattttttc tgatgaatcg agccaacaga aaacgctgaa
    17101 aaaacatcca aaagatggaa aaactcgccc gtaacgacca ggattatgat tgcccgcaaa
    17161 aggcgggcca ggacaatcca tagccgatat ccaatcggaa tttacgggag catagtaatg
    17221 acagatattg cgcagttgct tggcaaagac gccgacaacc ttttacagca ccgttgtatg
    17281 actattcctt ctgaccagct ttatctcccc ggacatgact acgtagaccg cgtgatgatt
    17341 gacaataatc gcccgccagc ggtgttacgt aatatgcaga cgttgtacaa caccgggcgt
    17401 ctggctggca caggatatct ttctattctg ccggttgacc agggcgttga gcactctgcc
    17461 ggagcttcat ttgctgctaa cccgctctac tttgacccga aaaacattgt tgaactggcg
    17521 atcgaagcgg gctgtaactg tgtggcatca acttacggcg tgttggtgtc ggtatcgcgg
    17581 cgctatgcgc atcgcattcc atttctcgtc aaacttaatc acaacgagac gctaagttac
    17641 ccgaatacct acgatcaaac gctgtatgcc agcgtggagc aggccttcaa catgggcgcg
    17701 gtggcggttg gtgcgactat ctattttggc tcggaagagt cacgtcgcca gattgaagaa
    17761 atttctgcgg cctttgaacg cgcgcacgag ctgggcatgg tggcagtgct gtgggcctat
    17821 ttgcgaaact ccgcctttaa gaaagatggc gttgattacc atgtttccgc cgacctgact
    17881 ggtcaggcga accatctggc ggcaaccata ggtgcagata tcgtcaaaca aaaaatggcg
    17941 gaaaataacg gcggctataa agcgattaat tacggttata ccgacgatcg cgtgtacagc
    18001 aagttaacca gcgaaaaccc gattgatctg gtgcgttatc agttagctaa ctgctatatg
    18061 ggccgggctg ggttgataaa ctccggcggt gctgcaggcg gtgaaactga cctcagcgat
    18121 gcagtgcgta ctgcggttat caacaaacgc gcaggcggaa tggggctgat tcttggacgt
    18181 aaggcgttca agaaatcgat ggctgacggc gtgaaactga ttaatgccgt gcaggacgtt
    18241 tatctcgata gcaaaattac tattgcctga tacgcctcat acgaacagca ccaccccgta
    18301 ggacggataa ggcgtttaca ctgcatccgg caacctatgc ctgatgcgac gcggaagcgt
    18361 cttatcatgc ctacatagca ctgccacgta tgtttacacc gcatccggca taaaaacacg
    18421 cgcactttgc tacggcttcc ctatcgggag gccgtttttt tgcctttcac tcctcgaaca
    18481 attttcatat tgtcgttttt gtgatcgtta tctcgacatt taaaagcaaa taatttcatt
    18541 atattttgaa atcgaaagca aacgacaaga tatgaaaatg tacgtggtat cgacaaagca
    18601 gatgctgaac aacgcacagc gcggcggtta tgcggttccg gcattcaata ttcacaatct
    18661 cgaaacgatg caagtggtgg tggaaaccgc tgccaacctg catgcgccgg tcatcatcgc
    18721 cggaacgcct ggcacattta ctcatgctgg tacagaaaat ctgttggcgc tggtcagcgc
    18781 gatggcgaag caatatcacc atccactggc aattcatctc gaccatcaca cgaaatttga
    18841 cgatatcgct cagaaggttc gttctggcgt gcgctcagtc atgattgacg cctcgcattt
    18901 gccttttgcg caaaatattt cacggctcaa agaggtggtg gatttttgcc atcgctttga
    18961 tgtcagcgtc gaggcggagc tggggcaact tggcggccag gaagatgatg tgcaagtcaa
    19021 tgaagccgat gcgttgtaca ccaacctcgc acaggcgcgt gaatttgccg aggcaaccgg
    19081 gattgattcc ctggcggtcg ccatcggcac ggctcacggt atgtatgcca gcgcaccggt
    19141 gcttgatttt tctcgactgg agaacattcg ccagtgggtg aacttaccgc tggtgctgca
    19201 tggcgcgtca ggattatcga ctaaggatat tcagcaaacc atcaaactgg ggatatgcaa
    19261 aatcaacgtt gcaacggagc tgaaaaatgc cttctcgcag tcgttaaaaa attacctgac
    19321 cgagcacccc gaagcgaccg atccccggga ttatttgcag tcggctaaat ccgcaatgcg
    19381 cgatgtggtg agcaaagtga ttgccgattg tggctgcgag ggcagggcat aacgcacctg
    19441 ccatttaaca aggaaaaaac atgaaaacgt taattgcccg gcataaagct ggtgaacata
    19501 tcggcatatg ttcagtctgt tctgcccatc cgttggttat cgaagcggcg ctggcatttg
    19561 atcgcaacag cacgcgcaaa gtgctgattg aagcaacgtc aaaccaggtc aatcaatttg
    19621 gcggttatac cggaatgaca ccggcagact ttcgcgaatt tgtttttacg attgccgata
    19681 aagttgggtt tgcacgcgaa cgcattattc tcggcggcga tcatctgggg ccaaactgct
    19741 ggcagcaaga aaatgcggat gcggcgatgg aaaaatccgt cgagctggta aaagcatatg
    19801 ttcgtgctgg cttcagtaaa attcatcttg atgcgtcaat gtcctgcgcg ggggatccca
    19861 taccgttagc accagaaacg gttgcggaac gagctgttgt gctttgcttt gctgcggaaa
    19921 gtgtggcgac agattgccag cgtgagcaac tgagctatgt cattggcact gaagttccgg
    19981 ttccgggcgg tgaggccagc gccattcagt cagtacacat cacccatgtt gaagatgccg
    20041 ccaatacttt acgtacgcat caaaaggcct ttattgcccg tgggctgaca gaggcgttaa
    20101 cacgtgtgat tgccatcgtg gtgcagccgg gtgtggaatt tgatcacagc aatattatcc
    20161 attatcagcc gcaggaagcg caggcgctgg cgcaatggat agaaaacacc cgaatggttt
    20221 atgaagcaca ttctaccgat taccagaccc ggacggctta ttgggaatta gtccgcgatc
    20281 actttgcaat attgaaagtc ggtcccgcat taacctttgc tttacgcgag gcgatatttg
    20341 cactggcaca aattgagcag gaacttatcg cccccgaaaa tcgcagcggt tgcctggcgg
    20401 taattgaaga agtgatgctc gacgaaccgc aatactggaa aaaatattat cgcacgggtt
    20461 ttaacgattc attactggat attcgttaca gcctgtcgga tcgtattcgt tattactggc
    20521 cgcatagtcg gattaaaaat agcgttgaaa cgatgatggt gaatctgcaa ggcgtggaca
    20581 tcccactggg catgattagt cagtatcttc ccaaacaatt tgaacgcatt cagtccgggg
    20641 aattatcagc aatgccgcat cagcttatta tgaataaaat ttatgatgtt ttgcgcgcct
    20701 atcgctacgg ctgtgcggaa taaggacggt atatgactaa cctgtttgtt cgtagcggaa
    20761 tttcttttgt cgatcgtagc gaagttttaa cctatatcgg taatgagatg ctcgcgaaag
    20821 gtgtggttca tgatacctgg ccacaggcat taattgccag agaagcagaa ttccctaccg
    20881 ggataatgct tgagcagcac gccattgcaa taccgcattg tgaggcgatt catgctaagt
    20941 cgtcagccat ttatctgtta aggccaacaa ataaagttca ttttcagcaa gcggatgatg
    21001 ataacgacgt ggcggtatcg ttggttattg cgttgattgt ggaaaatccg cagcagcaat
    21061 tgaaactttt acgctgttta tttggcaagt tacaacagcc cgatatcgtc gagacactaa
    21121 ttactcttcc tgaaactcag ttaaaggaat acttcacaaa gtatgtttta gattcagacg
    21181 aataaatccc tctgtaacaa taaaaaggac tatttatgaa acgcaagatt attgtcgctt
    21241 gcggaggcgc ggttgcgacc tctacgatgg cggcggaaga aattaaagag ttgtgtcaga
    21301 gtcataatat tcctgttgaa ttaatccagt atcgggttaa tgaaatagaa acctatatgg
    21361 atggtgtgca tttgatatgt accactgcca gggtggatcg tagttttggc gatattccgt
    21421 tagttcacgg catgcctttt gtttctggtg tcggtatcga agcattacaa aataaaattc
    21481 tgactatctt acaggggtga cctatgtttt cagaagtcat gcgttatatt ctcgacctcg
    21541 gccctacagt gatgctgccg gttgtcatca ttattttttc taaaatatta ggcatgaagg
    21601 caggcgattg ctttaaagcg ggtctgcata tcgggattgg ctttgttggc attggccttg
    21661 tgattggctt aatgctggat tccattggtc cggcggcgaa agcgatggcg gaaaatttcg
    21721 acctgaatct gcatgtggtc gatgtcggct ggccgggctc ttcaccaatg acctgggcgt
    21781 cgcaaattgc gctggtggcg attccgattg cgatattggt taacgtggcg atgttactga
    21841 cccgtatgac gcgggtggta aatgttgata tctggaatat ctggcatatg accttcaccg
    21901 gcagcgttgc tgcacctggc aaccggttca tggatgatag ggatggcggg tgtggtaatt
    21961 cacgcggcgt ttgtttataa gctcggcgac tggtttgccc gcgatacccg aaatttcttt
    22021 gagctggaag gtattgctat tccgcacggt acgtcggcgt atatggggcc gattgcggtg
    22081 ctggtcgatg ctatcatcga gaaaatccca ggcgttaacc gaattaaatt tagcgccgac
    22141 gatattcagc gcaaatttgg cccttttggc gaacctgtca ccgtgggttt tgtgatgggg
    22201 ctgattatcg gcatcctcgc gggttacgat gtcaaaggtg tattgcagct ggcggtaaaa
    22261 acggcggcag ttatgctgct gatgccacgg atgattaaac ccatcatgga tggtttaacg
    22321 cccatcgcta agcaggcccg tagtcgttta caggcgaagt tcggcggtca ggagttcctg
    22381 attggcctgg atcccgcatt actgctgggg catacggcgg tggtatcggc aagcctgatt
    22441 tttattccgc tcaccatttt aattgctgtt tgtgttccgg gtaatcaggt actgccgttt
    22501 ggcgatcttg ccactatcgg cttcttcgtg gcgatggcgg tcgcggtgca tcgtggaaat
    22561 ctgttccgca ccttaatctc gggtgtcatc attatgagca tcaccctgtg gatcgcgacg
    22621 caaactattg gtttgcacac ccaactggcg gctaatgctg gggcgttaaa agccgggggt
    22681 atggtggctt caatggatca gggcggttct cccattacct ggttactgat tcaggttttc
    22741 tccccgcaaa atattcccgg tttcattatt atcggcgcaa tttatctgac cggtattttc
    22801 atgacctggc gtagagcgcg tggttttact aaacaagaga aagccgttct cgcagaataa
    22861 tttttacctg agggggaggt tatccccctt aacaatcagg agtttttatg aaatcagtag
    22921 tgaatgatac tgatggtatc gtgcgcgttg caaaaagcgt cattcctgaa attaaacatc
    22981 aggatgaggt gcgggtaaaa attgccagct cgggcttatg tggttccgat ttacccagga
    23041 tatttaaaaa tggtgcacat tattatccaa taacgttagg ccatgaattt agcggctata
    23101 ttgatgcggt gggatccggt gttgatgatt tacaccctgg cgatgcggtt gcctgtgtgc
    23161 cgttattacc ctgttttact tgtccagagt gtttgaaagg gttttattcc cagtgcgcaa
    23221 aatatgattt tattggctcg cggcgtgatg gtggatttgc tgaatatatt gtcgttaagc
    23281 gaaaaaatgt ctttgctcta cccacggata tgcctattga ggatggggct tttattgagc
    23341 cgattaccgt tggcctgcat gcttttcatt tagcgcaagg ttgtgagaat aaaaacgtta
    23401 ttattattgg tgccggaacc attggcctgc tggctattca gtgcgctgtc gcgctgggag
    23461 caaagagtgt gacggcgatc gacattagct cagaaaaact ggcactggca aaatctttcg
    23521 gtgcgatgca aacatttaac agtagcgaaa tgagcgcgcc gcaaatgcag agcgttttac
    23581 gcgaactgcg ctttaatcag cttatcctcg agacggctgg cgtaccgcaa actgtcgaac
    23641 tggcggtaga gattgccggt cctcatgccc aactggcgct ggtgggcacg ttgcatcagg
    23701 atctgcattt aacatcggca acgtttggca aaatattgcg taaagagctg acggttatcg
    23761 gcagttggat gaactattcc agcccttggc cggggcagga gtgggaaacg gcgagccggt
    23821 tgctgacaga acgtaagtta agcctggatc cattaatcgc tcaccgtgga agctttgaaa
    23881 gcttcaccca ggcggtgcgt gacatcgctc gtaatgccat gccgggcaaa gtgttgctca
    23941 ttccatgaaa ccgcgggcca gcgtgatgct ggcccggtat tgtgcaaaac agatcattca
    24001 ccaatggtcc cccttcgttt acactagcca caattgaata tgggtaaatg acgatgaatt
    24061 cattcgagcg aaggaataag atcatccaat tagtgaatga acagggaacc gtgcttgttc
    24121 aggatctggc gggagtattt gctgcctcgg aagcgacaat ccgtgccgat ttgcgctttc
    24181 tcgaacaaaa aggcgtggtt acgcgctttc atggcggtgc ggcgaaaata atgtccggta
    24241 atagtgaaac cgagacccag gaagtcgggt ttaaagagcg atttcagctc gccagcgcgc
    24301 caaaaaacag aatagcgcag gaggcagtca aaatgatcca tgaagggatg accgttattc
    24361 tcgacagcgg aagtaccaca atgcttatcg ccgaaggatt aatgaccgct aaaaatatca
    24421 cggtgattac caacagtctc ccggcagcgt ttgccctttc cgaaaataaa gacattactc
    24481 tggtcgtctg tggtggcacg gtccgccata aaacgcgctc gatgcatggt tccattgccg
    24541 agcgttcatt gcaggatatt aatgccgatt taatgtttgt cggtgctgat ggtattgatg
    24601 cggttaatgg tattacgacc tttaatgaag gttattcgat tagcggggcg atggtcaccg
    24661 ccgcaaataa agtgattgcc gttctcgatt catcgaaatt taatcgtcgc ggttttaatc
    24721 aggtactgcc aatcgaaaaa attgacatta ttgttaccga tgatgctgtg tcagaggtgg
    24781 ataaattggc attgcagaag acacgggtaa aattaattac ggtgtagtat tgttctgtat
    24841 gccggataag gtgtttacac cgcatccggc attacggatt ctccctggtt caagagagaa
    24901 tagtttgatt aacgcaataa tggacaatct ggtggtaatc gacaacgcaa cgccgccgga
    24961 agtatttcaa tatggaagtt ttgcccactc aacggttcgc catcaagatt aaaagtgatt
    25021 tcgtgtgggg cttgtatatc aaaccacgac gaagcgcctt cgataatatt cgggttatct
    25081 tcgtcagatt ttaaggttga tacgagagcc ggaagaattt catcgccggt aaaaatgcgc
    25141 agttgcagca agccatcgtt aattaacgcg ttcgggcaca attgttgacc gccaccggcc
    25201 tgacgcccgt taccaatacc aatgaccagg gcgtcacctt gccagtgaaa gttttcaccg
    25261 cggatttcac aacggtccgg ttgcagagta tccatgcgca ttaagccatg aatgatgtaa
    25321 gagacgccac ccagcgcggc ttttaatttt tccggcgttt ctgtggtaat acgcgtccca
    25381 aatccgcctg tcgccatatt aataaaacag gtttgtttgt tgacctgcgc catatctatc
    25441 gcaatggcat taccggcaat tgccagtttc agcgccttat ccagtgcctc aggaatccct
    25501 acactggtgg caaaatcatt ggcggttcct aatggcaaaa ttcccagcgc gggtatgtca
    25561 tccccctcac actgaatcaa cgccgtagaa acttcattaa tggtgccatc gccaccaccg
    25621 gcaatcaccg ttgcgacgcc caacttccgg gcctcctcta caaatcgtgc ggcatcacct
    25681 ttctcccagg tgacccgcac atggatcgtc attccttcct cacgcaacag cataattgct
    25741 tcgcgcaagg gtagattgtc agtacttttg ccattaagaa tcagtaagct ggcgggaaat
    25801 tctgccatga tcgtatgtgc ctttatgatt ggtctgtaga tagtgtagag cagaaaacaa
    25861 aaggtggagt cagaacagga tgaaagtcgg aggataaaaa gtcagttcaa gtatggagga
    25921 ttcagtggtt accaccagga gcttgcaatg gggacagcga atactaacgg ctgccaccga
    25981 taaatttcaa aaaagagcat atacctaata ttcaactaaa cagtggcatc ttcaatataa
    26041 tatattaaag cccccatgga gttaccctga agggcctcaa tgtccgtaat tcctacttat
    26101 gtaggaaatg ttgtacagaa catttattat aatcctattc aattataata atcatgccat
    26161 tattatattt aaacactaga gagtgtcgtt ggtatttaat gggggaaggt gagatgaaaa
    26221 agatagctgc tatatcatta attagtattt ttcttatatc tggttgtgct gtgcataatg
    26281 atgaaacaag tatcggtaaa tttggtcttg catataaaag taatattcag cgtaaactcg
    26341 ataaccaata ctacaccgaa gccgaagctt ctttagccag gggcagaata tctggtgcag
    26401 aaaatatagt aaaaaatgat gcagcccatt tctgtgttac tcagggcaaa aaaatgcaga
    26461 tagttgacct gaagacagaa ggtgcaggat tacatggcgt cgctcgtctg acattcaaat
    26521 gtggagagtg agaatatttt ttggtaagcg tcaaacatgc gcgttctggc tgtgcgtagc
    26581 cggaacctgg gcgagcacga taccgttacg tgaaaggcat tgtgctatga agggggattc
    26641 tatcgatgtg gtcaatggaa gacggttatc agggatcaat ccttctttac agcttatgta
    26701 caaaaaataa gcccgtgtaa gggagattac acaggctaag gaggtggttc ctggtacagc
    26761 tagcatttta tggattatgt ttttcagcga aacggatgat aaccttaata aatgcagctg
    26821 tatgtgatcg gtttctaaga attttccatc cgggaaaaat aatcgaaatt aatcacttac
    26881 cgtgggggtt acgcgtggtt tccccggaga aattacgcat tagcagcgcg taatttagct
    26941 caagatcctg cgggaccggg agccacacag tataaccatc gcctggtgcg accggcatag
    27001 cttcgccttt ggcgttttcc atgtgctcaa gggtaaagtt aatgttgcct tgcggcgtca
    27061 tcagctcaag actgtcgcca acggagaatt tatttttcac cgctaccgct gcgaggtccc
    27121 ccttgcgctc accggtaaac tcaccaacaa actgctggcg gtcagaaact gaataaccgt
    27181 attcgtagtt ctgataatcg tcgtgagtat gacgacgcag gaaaccttcg gtatagccac
    27241 gatgcgccag accttccaga gtttccagca ggctggtatc gaacggtttt cccgcagcgg
    27301 cgtcatcgat agctttgcgg taaacctgtg cggtgcgtgc gcaatagtag aaagatttgg
    27361 tacgaccttc gattttcagc gaatgcacgc ccattttggt caggcgttct acatgggcga
    27421 tggcgcgcag atctttcgag ttcatgatgt aagtgccgtg ctcatcttca aacgcggtca
    27481 tatactcgcc cggacgctgg gcttcttcga tcataaacac tttgtcggtt ggcgcgccga
    27541 tacccagcgt cggctcaaca ttttgcaccg gaatcggctc gtacttgtgt acgatgttgc
    27601 caacgtcatc ttctttccct tcctggacgt tgtactccca gcggcaggcg ttggtgcagg
    27661 tgccctggtt cgggtcgcgc ttgttgatat agccagagag caggcagcga ccggagtagg
    27721 ccatgcacag tgcgccgtga acgaagatct cgatctccat atccggcacc tgattatgga
    27781 tctcttcaat ctcttccagc gacagctcgc gagagaggat cacgcgggtc agtcccattt
    27841 gctgccagaa tttcaccgtc gcccagttca cggcgttagc ctgcaccgag aggtggatcg
    27901 gcatttctgg gaagtgctca cgcaccagca taatcagccc tggatcggac ataatcagcg
    27961 catccggccc catttccacc accggtttca ggtcacggat aaaggttttc agcttggcgt
    28021 tgtgtggtgc aatgttgacc acgacataaa actttttccc cagcgcgtgg gcttcattga
    28081 tgccgagctg aagattttcg tggttgaatt cgttgttgcg cacacgtagg gagtaacgcg
    28141 gctggcccgc ataaacagca tctgcgccat aagcgaaagc gtaacgcata tttttcagcg
    28201 ttcccgccgg ggaaaggagt tccggtttaa acataattct ctcgttctga tgacaggtca
    28261 gatccgcttc acctgatgaa gcggttaggg gaggccccca ctttaagggc gggcattgta
    28321 gcgctgtagg ggggatctgg taaagcgccg gacgccaaca acccacgccc ggcatcgtaa
    28381 ttacgtattg tctttcaccg tctggcttgt gccattggct ttaacggagg caattccggt
    28441 ttcacgagat tgcgcggtgg cgtacatctg gctggagccg ataatttgat gattagcggc
    28501 tttcagattg aaatagaatt tgccgttttt ttctcatagc gctcttcttg cgggctgttg
    28561 ctacgcaccg acgcgatgcc cttttccgca gaggcttttg aggtatacag ctcgctggtg
    28621 aggatagtct caccattgcc cgcttttagc acaaaccgga actgattatc actgctctta
    28681 ctgagttcaa accaaccagc cataatgact ccttgattga aattgcttat cgaaaacagc
    28741 atctaaaggt gctgtttact gattaaagta tgagcgattt tctgatgcct gccagggcgg
    28801 agagataatg tcagtttgct gaccatgcgt ggaataatgt tgccggatcg ggtctgaacg
    28861 ccatctccgg ctaacaaagt aatgtcgcta aaactaaacg atgcggcagg cgtcggcttc
    28921 ccagcggtaa ccaacgccgt aaacggcgcg gataaatgac tgttcggcgt cgagagattc
    28981 cagcttgcgg cgcagatttt taatgtggct gtcaatggtg cggtcggtta ctacgcggta
    29041 gtcgtcataa agatgattga gcaattgctc gcgggagaac acttttcctg attcgtgaga
    29101 gagcgtttta agcagacgaa attccgcagg cgtcaggtca agcattttac cgcgccatga
    29161 agcctgaaaa cgaccttcgt cgataatcaa cgggctttcg gcatcctgtt gttgaagttc
    29221 gcgttgcggt ttacaacggc gcaaaatggt tttgacacgc gctaccactt cccgtgggct
    29281 gtacggcttg cagatgtagt catcggcgcc aatctccagc cccagcaggc ggtcgatctc
    29341 ttcgattttt gccgtcacca tcacgatcgg aacatcggaa aaacgacgaa tttcccggca
    29401 taatgtcagg ccatcggtgc cagggagcat cagatccaac aggatcagat ccggtggtgt
    29461 ctggcgcaca tacgccagta cctgatcgcc gtggctgata agcgtcggcg catagctcgc
    29521 agcacgcaga taatcaatga gcaactgccc cagcttcggt tcatcttcca cgatcaaaat
    29581 acgcggtgtg ttttcgtcga ttggtaactc ggtcatactt ctctctgtaa atcccgttcc
    29641 agcggtaact ctactgtaat gcttaccccg ccaaaaggcg aatgggcggc aataatgcgg
    29701 ccattatggg cttcaacaat gttcaggcaa atcgccagcc ccagcccgga accgccgctg
    29761 gcgcggttgc gggaaccttc ggtgcgataa aaacgttcaa acaatttttg tagctgatcg
    29821 tcactgacac ctggcgcact gtcggcaaag gtcaggcgca ccgttttgtc gtgctgctcg
    29881 gcagagattt tcaggctgcc gccgctgtca gtgtagcgca ggctgttttc cagtaagtta
    29941 ttgaataact gcattaaacg gtcgcgatcg ccaaatacgg tgatactgtc tggcagggaa
    30001 aattgcagtt tcaggccacg gctggcgaat cgttcgcgaa atgcgccgcc cgccacttcc
    30061 agcagtggga tcaaatctac cggtgctttt tgataggcga gagcgccttc atcagacatc
    30121 gacaactgat gaagatcgtc aaccagtttg gtcagtgtac cgacctccgc ctgtaaagac
    30181 gccaccgtct ccggcgtgaa tttgcgcacg ccatcctgaa tggcttccag ttcaccgcgc
    30241 agcaccgcta atggcgtacg cagttcgtga gaaatatcgg ccataaaatc gcgccgcatt
    30301 tgctggtttt tctccagcgt gctggcgagc tggttgaagt cttgcgccag tttgcccagt
    30361 tcatcttcac tggtgggcgt tacgcgggta gtgaaatcgc ccgccgccag tttgtgcgtg
    30421 ccatcgataa gtcgttttac cggtgccagc aaaccgcgcg ccagcagaaa ggtggcgagc
    30481 gccgcgagta acgttgccag ggcgacgatc agccagctgg tttgccgctg ttgtttatcg
    30541 aaattgatat cagtattgcg ggtcagccgc tcaacagggg aggcaatcac cgcgcccact
    30601 tccgcaccgt tgaccagaat gggtcgtcgt gtaccgtcag gtggaatcgg cgctcgcgga
    30661 ccaaccagta ctttgttgtt ttgatcgacc acccagaact gggtacgcca gccgtgcggc
    30721 ggcatacccg ggccgggttt atcttccgaa ttatcgtgtt caaatgaacg caggatctga
    30781 aagacaaagc gatcgttgtt gcgcaggaag cgccagttac catgctgcgc atactgctcg
    30841 ccaagcgcat cacttaacag ctgtaatcgc tgttcattgc cgtgtttgat gtaatcaata
    30901 aagccacgct caaaactgat acgcaccgcc cagtgcatac tgatcagcaa gacaatgcag
    30961 gtggcgaaaa tcgccagaaa cagtttgccg gtaataccgg gtcgccagaa cttcattgcg
    31021 cgctcctttt tcgccgcgaa atagctacat tttgatgcgt atcgttcggc actctggcaa
    31081 agatgaacgc cggaagggcg atgataaacg ccatgctaag ccaggtgtac ataaagacgg
    31141 tttgtgtggt gccgctgtcg acgctgacat gctgcgaacc aaaaagcccc agcaacagcc
    31201 cggcgatggt gacgccgata ctcatcgaca attgcataat catcgacagc aggctgtttc
    31261 cgctgctcgc cagattgtcc gggagatctt tcagcgtcag ggtgttcatg gaggagaaac
    31321 gggtcgagtt gaccatccct tgtaaaaaca ggacgaacgg caaaacgtag taccagccca
    31381 acagagcggt agtcataaac aacagggtga ccagcgacag acccagcgtg gtcgctacca
    31441 gtacccgacg ataaccaaag cgattcacca cctgtaccac aattcgcttc attcccatgc
    31501 tgccaagcac catcgggatc atcatcagtc cggcatgaaa cggcgagaaa ccgaggccaa
    31561 tttgcaggaa aaccggtgtc ataaagggca acatgccact gccaatacgt ccggcaaagc
    31621 tccccgccag gcccagcgaa aaggtacgag tgcggaacag tttcagactg aacagggcgc
    31681 ggttgttatt tctggcgtgc agcagataaa gcaccagtgc caccacgcca actgcgacca
    31741 ggccagcaat cgccagcggc gataaacctg tacctttact gccgtccagc gccagggtta
    31801 ataccgccat gccaaccgcc agcaataaaa agccggaaag atcaaagcgc cgcgtctgca
    31861 tggtgtagtt tggcattaac atcaatgtgg cgatcgcacc gataatcccc accggaatgt
    31921 tgatcaaaaa gatccagtgc cacgacgcgt attctaccag cagaccgccg agcgccggac
    31981 cgagtagcgg accgacctga ccgggtaacg tgacaaaggt catcgccgcc atatattgct
    32041 cgcgcggtac gattttcatc accgtcaacc tgccgaccgg caccatcatc gcaccgccaa
    32101 cgccctgtaa cgcgcgtgcc agcaacagtt cgttcagcgt gccggaaagc gcgcaaaaca
    32161 gtgaaccgag agtaaacagc acgatggcgg taaagaaaat attgcgcacg ccgactttgt
    32221 ccgccagcca gccgctggcg ggcagcatca ccgccacggt cagcacataa gagacaatga
    32281 ccatgtgcat atgcaacgga ctttccccga ggctttgcgc cattgaggga agggcggtgt
    32341 ttacgatggt ggtgtccagc gactgcataa agaagccgaa agccacaatc cataattgcc
    32401 agcgggtgct gtcgggaaga tctgtcattt actcggttac cgtttgttta ggtttacgcg
    32461 aaaaacgcag ccgcagacgg tcgaaaaaga gatacaccac cggcgtggta tacagcgtca
    32521 ggagctggct catcaccaga ccgccgacaa tggtgatcct caggggttgc cgcagctccg
    32581 agccgtcacc gcccgacaat accagcggca gcgcgccaaa cagcgccgcc agggtggtca
    32641 tcataatcgg gcgaaaacgc agcagacagg cctggaaaat agcttcctgc ggcgtcaggt
    32701 taccgtgccg ttgggcttca agcgcaaaat cgaccatcat aatggcgttt ttcttcacga
    32761 tgccgattaa tagcatgatc cctatcaggg cgattaggct gaacggggca ttgaacagct
    32821 ccagcgccaa cagcgctcca acgcccgccg agggcagggt ggagagaatc gtcagcggat
    32881 gtacgtaact ctcgtaaagg atacccagca cgatatacac cgtggcgatg gcggcgataa
    32941 tcaggatcac ctgcgagttc atcgtctcct ggaaaacctg cgccgtgccg gcaaaactgc
    33001 cgcgcaccgt cgaaggcaca ccaagctggg tcattgcgcg atcgatcgcc gcactggcgt
    33061 ccgagagcga ttttccggtc ggcaggttga acgagatggt cgaggccgcc gataatccct
    33121 gatgattcac cgatagtggg gcattcgccg gttgccattt agcgaaatac gacagcggga
    33181 tcgctttgcc ttcgttattg ataacgaaca ttttttccag cgcactgatg tcctgggtat
    33241 agcgcggatc cacttccatc accactttat actggttcat cggctggtaa atggtcgaga
    33301 tttgccgctg accgaaggcg ttatttaaca gactgttggc ggcctgtacg tcgattccca
    33361 gccgtgccat ggtgtcgcgg tcgtaaacca gattcatctc cgcgccgtta tcctgctgat
    33421 cggagttcac gtccgccagt tccggcaacg tcgccagttt tttgcggatt ttcggctccc
    33481 attcgcgcag tgccgccagg tcgtcggata acaacgtgta ctggtagctg gcgttcgact
    33541 gacgcccacc aacgcgaata tcttgtaccg ccatcaggaa cagattcgcc cccggttctt
    33601 tcgccagctt cacgcgcaga cggtcgataa tttgctgcgc cgtttcgctg cgttcgtcgc
    33661 gtggcttgag ggtgataaac atcatcccac tgttaactcg tgaaccgccg gtaaagccgg
    33721 tgacattatc cactgccgga tcgtcacgga taattttcat gaaatcctgc aatttgccgc
    33781 gcatcgcctg aaacgaaata ctctgatccg cctgtatccc acccatcagt acgccggtgt
    33841 cctgctccgg gaagaaggtt ttcgggatcg agatattcag cgcaatggtg ccaagcagca
    33901 ccacgcccac cagacgggta tgattgagca cccatttcag cgacttgccg tagccttgtt
    33961 gcagggctac caacatgcgg ccaaaaccac gcagtcgctt ttgctcacgc ggcttgctgg
    34021 cttttagcat ccagccgcac atcattggcg ttagcgtcag ggaaaccagc aacgaaatac
    34081 caatggcgac agaaagcgtc acggcaaact cgcgtaacag tcggcccggc aatccgccca
    34141 tcaacagcag cggcaggaac accgccacca gtgacagact catcgacagc accgtaaagc
    34201 cgacttcgcg agtaccttgc agcgcggcct gtagtggttt catccccgct tccagatgac
    34261 gggcaatgtt ttccagcacc acaatggcgt catccaccac gaaaccagta gcaatggtga
    34321 gcgccatcag cgaaagatta ttgagactga atccgcacag gtacatcgcc gcaaacgtac
    34381 caatcagcga aaccggcacc gcaacggcgg gaataatagt ggcgcgaccc gagcgcagga
    34441 ataaaaagac caccagaatc accagcgcca ccgagataat cagcgtttgc tcgacttctt
    34501 ccagcgaggc gcgaatggtg ggggagcgat cctgggcaat ttgcagatca atcgccgccg
    34561 gaatggtctc ctgcaactct ggtaattttg cccggatgct gtctaccgtc tggataatat
    34621 tggcttccgg cagtttgcgg atcatcagta aaatagccgg tttggcgttg gtcatcccgg
    34681 cgttgcgcac atcctgcact gagtcagtca ccgtcgccac atcgcccaga cgaaccgcgc
    34741 cgccgttgtt gtagtgaata atcagcggct gatattcagc ggcggttttc agttcatcgt
    34801 tggtctggat ctgccagcgg tgagtgccat cttccagcgc cccctgcggt ttacgcacgt
    34861 tggcattgct gatggcagtg cgtacgtcgt ccagcgacac gccctgatta aacagcgcct
    34921 gcgggttcag cccgacgcgc acggcgggca gcgagctgcc accgacatcg acatcaccga
    34981 caccgtcgat ttgcgaaatc gtcggagcca gctgcgtcga ggcgaaatcg tacagttcac
    35041 cctgcgaata agtatcggat gtcagcgtga ggatcataat tggcgcatcc gacgggttcg
    35101 ctttgcgata ggtcgggcgg ctgggcatcc cgctgggcag caaactttgt gcagcgttga
    35161 tcgccgcctg cacatcacgc gctgcgccgt tgatatcccg gtcaaaatca aactgcaaaa
    35221 taatgcgcgt gctgccgagc gaactgctgg aggtcatttc actaaccccg gcaatgcgcc
    35281 caagttagcg ctccagcggc gtggcaacgg aagacgccat tgtctccggc gacgcaccgg
    35341 gcagcgaggc gctgaccata atcaccggaa aatcgacctg cggcagcggg gcgaccggca
    35401 gcatacggaa acccagtatg ccgcacaggg taatggcgac cgacagtaaa atcgtcgcca
    35461 ccgggcggta aatgaagagg gcaaaaaact tcacttacgc ctcctcttca tggcgagcaa
    35521 agcgactttt ggtccacaat accaagcggt cgaacagcag ataaatcacc ggcgtggtga
    35581 acagcgtcag cacctggctg acaatcagac cgccgaccat gccgatacct aacggacgac
    35641 gcagttccgc gccgaccccg gtactcaaca tcagcggcag cgcgccaagc agagccgcca
    35701 gggtggtcat caggatcgga cgaaaacgca acagacaagc ctggtagatt gcatcgcgcg
    35761 gcgacatgcc ttgctcgcgc tcagcagcca gcgcgaagtc gatcatcatg atggcgttct
    35821 tcttcacaat accgatcagc aaaataatgc cgataatcgc aatcacatcc agttcgctac
    35881 cggcaatcat taatgccagc agcgcgccga cccctgcggt gggtagcgtc gagagaatgg
    35941 tgatcgggtg aataaagctc tcgtacagaa tgccgagcac gatatacatc gccaccaccg
    36001 ccgcgacaat cagccagaca gtgctgccca gcgccgactg gaaggcgagg gtgctgccct
    36061 ggaattgcgt ggtgatatcc accggcagat tcagcgtctt ttcggtgtcc ataatcgcct
    36121 gcaccgcatc gcccagcgaa tagttatccg gcacgttaaa ggagatggtt gttaccggga
    36181 actgatccag atggttgatg gaaagcggcg caaaacgctg ctcaattttg gcaattgagc
    36241 ttaacggcac cacgccaccg tcgctgctgg tcaggcgaat ggtatccagc gccgcgaggc
    36301 ctggggtatt ttcggtattg tgctccagca ccacgcgata ctggttagcc tgagtataaa
    36361 tagtggaaat cagtcgctga ccaaacgcgt tgtacagggc gttatcgacg tccgccatgc
    36421 tgatccccag acggctggcg ctgtcgcgat caacattgac atacgccacc agccctttgt
    36481 cctgccagtc gctggagaca tcagaaagct gtggcagttg ctggagtttt tccatcaact
    36541 gtggcaccca ggtactgagc gcatccagtg acgtggcctg caaggtaaac tggtactggg
    36601 tgcggctgac ctgagtatcg atggtcaggt cctgcgttgg ttgcaggaag agatcgacgc
    36661 ccggcacttt atctaccgcc gtttgcagac gggcgatgac tttttgcacc cgatcatcac
    36721 gttcatccaa cggtttgagg ttgatttgta aacgtgcact attcagcgac gggttagtgc
    36781 catcaacgcc aacaaatgag gtcaggcttt gcactgccgg atcctgcaaa atcacgtccg
    36841 cgacctggcg ttgtcgctgg gccatattgg caaaggagct ggattgcggt gcctgcaaag
    36901 tgccctgaat aatgccgttg tcctgtaccg ggaagaaacc tttcggaatg aacacccaca
    36961 gcagcacgct aagcagcagc gtgctgagtg ccacgcttaa ggtcagccac ggatgattca
    37021 gcactttcgc cagtccacga ccataggcgg cgattatcct gtcgaacatt ttttccgagg
    37081 cacgggagaa gcggttctgt ttacgcaacg actcctggct gagcatccgc gcgcacatca
    37141 tcggtgtcag ggtcagcgac accaccgctg agatcaaaat cgctaccgcc agggtaatag
    37201 caaattcgcg gaacagtcgc ccgacgatat cgcccataaa cagcagtggg atcaacaccg
    37261 caatcagtga gaaggtcagc gagataatgg taaagccgat ttcacctgcg cccttgagcg
    37321 ccgccgccaa cggtttttcg cctttttcga tatagcggga aatgttttcg atcaccacga
    37381 tggcgtcatc gaccacgaat ccggtggcga tagttaacgc catcagtgtc aggttattga
    37441 ttgaaaaatc gagaaacacc ataaccgcga aagtgccgat taacgacagc ggtacagcaa
    37501 caccgggaat gatggtcgcc ggaatattgc gcaaaaacag gtagataatc atgactacca
    37561 gcgcgatagc catcatcaat tcaaactgag tatcatcgac ggatgcgcgg atattggtgg
    37621 tgcgatcgga aagcactgtc accttcaccg atttcggcag actctcagtg agctgtggca
    37681 gcatctgccg aatgctgtcg gcggtggaga taatgttagc accgggctgg cgctgaacat
    37741 tcatcacaat ggcctgttct ttgttcgccc acgcgccgag ccagctgttt tctgcacctt
    37801 gctctacagt tgctacatcg cccagacgaa ttggcgcgcc gttctggtag gcgatgatta
    37861 gctggcgata ctcttcggcg gattgcatct ggtcgttcgc ggaaagcgtg accgcacggg
    37921 aagggccgtc gaggctacct tttgccgagt taacgttagc gccggtaatg gcggtgcgca
    37981 cggtttcgct ggtcaggccg agggcggcaa tcgcctgagc gttaagtttg acgcgaacag
    38041 ccggacgctg accgccggaa agcgtcacca ggccgacgcc ggaaatctgc gagattttct
    38101 gcgcgacgcg ggtttccacc atatcttcca cttgcgtcat cggcatggcg gttgaggtga
    38161 cggcgagcgt catgatcggc ggatctgccg ggttcacttt gctgtaaacc ggcgggttag
    38221 gcagatcgct cggcaacaag ttggtcgcag cgttaatcgc ggcctgcact tcctgctcgg
    38281 cgacattgag cggtaatgtt agctggaact gcaaagtgat aactgacgca ccgccggaac
    38341 tttgcgacga catctgtttc aggccagaca tctgcccgaa ctggcgttcg agcggcgcgg
    38401 taacggcaga ggtcatgaca tccgggctgg cacttgggta gagcgtgacc acctgaatgg
    38461 tcggatagtc cacttccggc agcgccgaaa cgggcagggc gcgataaccg ataatcccgg
    38521 cgagtaagat cgccaccatc agcagcgtgg tggcgacagg acgcataata aacaggcgcg
    38581 acgggccgcc tgtgctgctc gggggtaaca cctgcatcag gagcgtgctc cttttttcgc
    38641 gtattcgcgg ctggtggctt tctcttccgg agtggtggcg ctctgggctt ccaccacttc
    38701 cactttctga ctgtcctgaa tgcccggcgt caccagatgt ttgctgacct tgttttcgct
    38761 attcagcacc cagacaaaat ggccttcatt gcccatttgc agggcggctg ttgggatcac
    38821 tacggcgttt tgttcggtgt cgactaacat gcgcgcgtta acaaactgat tgggaaacag
    38881 cgcatcatcc tgattattaa agcgtgcttt cactttaatc gtaccggttg tggcatcgat
    38941 ttggttatcg aggcttaaca gcgtgccttc acttaatttc ttcgaattgg tgcgatccca
    39001 ggcttctacc atcagcggtt ttccggcttt ttgcgcctgc actacagtag cgatatcgct
    39061 ttccggcagg gtaaagacta aatcgatagg atgcgtctgg gtgatcacca caattccggt
    39121 ggtatcacca ctggagattt ggttaccaac atcaacttgc ttgagaccaa cgcgaccatc
    39181 gactggtgcg gtgatgcggc tccagtcgag ttgcaactgc gcgctggcga cgcttgcttc
    39241 atcagcctta atggtgcctt cggtttcact gaccagcgcc tgttgggcat ccagctcctg
    39301 acgggagacg agattggttt ttgccagttg ctgataacgc gccagatcac ggcgggcgtt
    39361 agcaagcgtg gctttatctt ttgccagttg gccctgggtt tgtgctaatg caactttgaa
    39421 ctggctgggg tcaatttctg ccagtaaatc gcctgctttg acctgctggc cttcctggaa
    39481 atgtaacgcc atcagttgac cgtccacgcg gctgcgcacc gtaacagtat tagcggcggt
    39541 aatggtgcca agcccggt
//
LOCUS       DAETPZ010000028        38755 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.028, whole genome shotgun sequence.
ACCESSION   DAETPZ010000028 DAETPZ010000000
VERSION     DAETPZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.028"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="K0W59_001687"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="K0W59_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HBI3022764.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            530..580
                     /gene="hisL"
                     /locus_tag="K0W59_001688"
     CDS             530..580
                     /gene="hisL"
                     /locus_tag="K0W59_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HBI3022765.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    556..679
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            726..1625
                     /gene="hisG"
                     /locus_tag="K0W59_001689"
     CDS             726..1625
                     /gene="hisG"
                     /locus_tag="K0W59_001689"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBI3022766.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1631..2935
                     /gene="hisD"
                     /locus_tag="K0W59_001690"
     CDS             1631..2935
                     /gene="hisD"
                     /locus_tag="K0W59_001690"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBI3022767.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2932..4002
                     /gene="hisC"
                     /locus_tag="K0W59_001691"
     CDS             2932..4002
                     /gene="hisC"
                     /locus_tag="K0W59_001691"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBI3022768.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            4002..5069
                     /gene="hisB"
                     /locus_tag="K0W59_001692"
     CDS             4002..5069
                     /gene="hisB"
                     /locus_tag="K0W59_001692"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HBI3022769.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KEGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5069..5659
                     /gene="hisH"
                     /locus_tag="K0W59_001693"
     CDS             5069..5659
                     /gene="hisH"
                     /locus_tag="K0W59_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBI3022770.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5659..6396
                     /gene="hisA"
                     /locus_tag="K0W59_001694"
     CDS             5659..6396
                     /gene="hisA"
                     /locus_tag="K0W59_001694"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBI3022771.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGISLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6378..7154
                     /gene="hisF"
                     /locus_tag="K0W59_001695"
     CDS             6378..7154
                     /gene="hisF"
                     /locus_tag="K0W59_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBI3022772.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7148..7759
                     /locus_tag="K0W59_001696"
     CDS             7148..7759
                     /locus_tag="K0W59_001696"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBI3022773.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7945..8922)
                     /gene="wzzB"
                     /locus_tag="K0W59_001697"
     CDS             complement(7945..8922)
                     /gene="wzzB"
                     /locus_tag="K0W59_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HBI3022774.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9068..10234)
                     /gene="ugd"
                     /locus_tag="K0W59_001698"
     CDS             complement(9068..10234)
                     /gene="ugd"
                     /locus_tag="K0W59_001698"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HBI3022775.1"
                     /translation="MKITISGTSYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRTSSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(10484..11890)
                     /gene="gndA"
                     /locus_tag="K0W59_001699"
     CDS             complement(10484..11890)
                     /gene="gndA"
                     /locus_tag="K0W59_001699"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBI3022776.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11999..12358)
                     /locus_tag="K0W59_001700"
     CDS             complement(11999..12358)
                     /locus_tag="K0W59_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022777.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(13008..13571)
                     /locus_tag="K0W59_001701"
     CDS             complement(13008..13571)
                     /locus_tag="K0W59_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022778.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPSIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13779..14717)
                     /locus_tag="K0W59_001702"
                     /pseudo
     CDS             complement(13779..14717)
                     /locus_tag="K0W59_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14780..15154)
                     /locus_tag="K0W59_001703"
     CDS             complement(14780..15154)
                     /locus_tag="K0W59_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HBI3022779.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15158..16306)
                     /gene="wzy"
                     /locus_tag="K0W59_001704"
     CDS             complement(15158..16306)
                     /gene="wzy"
                     /locus_tag="K0W59_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HBI3022780.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFLCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16326..17231)
                     /gene="rfbG"
                     /locus_tag="K0W59_001705"
     CDS             complement(16326..17231)
                     /gene="rfbG"
                     /locus_tag="K0W59_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HBI3022781.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGQKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17224..18114)
                     /locus_tag="K0W59_001706"
     CDS             complement(17224..18114)
                     /locus_tag="K0W59_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HBI3022782.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18144..19400)
                     /gene="wzx"
                     /locus_tag="K0W59_001707"
     CDS             complement(18144..19400)
                     /gene="wzx"
                     /locus_tag="K0W59_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HBI3022783.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19416..19961)
                     /gene="rfbC"
                     /locus_tag="K0W59_001708"
     CDS             complement(19416..19961)
                     /gene="rfbC"
                     /locus_tag="K0W59_001708"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBI3022784.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19966..20844)
                     /gene="rfbA"
                     /locus_tag="K0W59_001709"
     CDS             complement(19966..20844)
                     /gene="rfbA"
                     /locus_tag="K0W59_001709"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBI3022785.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20903..21802)
                     /gene="rfbD"
                     /locus_tag="K0W59_001710"
     CDS             complement(20903..21802)
                     /gene="rfbD"
                     /locus_tag="K0W59_001710"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBI3022786.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21802..22887)
                     /gene="rfbB"
                     /locus_tag="K0W59_001711"
     CDS             complement(21802..22887)
                     /gene="rfbB"
                     /locus_tag="K0W59_001711"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBI3022787.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(23260..24153)
                     /gene="galF"
                     /locus_tag="K0W59_001712"
     CDS             complement(23260..24153)
                     /gene="galF"
                     /locus_tag="K0W59_001712"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HBI3022788.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24328..25722)
                     /gene="wcaM"
                     /locus_tag="K0W59_001713"
     CDS             complement(24328..25722)
                     /gene="wcaM"
                     /locus_tag="K0W59_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HBI3022789.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLNHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFIRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25733..26953)
                     /gene="wcaL"
                     /locus_tag="K0W59_001714"
     CDS             complement(25733..26953)
                     /gene="wcaL"
                     /locus_tag="K0W59_001714"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HBI3022790.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26950..28230)
                     /gene="wcaK"
                     /locus_tag="K0W59_001715"
     CDS             complement(26950..28230)
                     /gene="wcaK"
                     /locus_tag="K0W59_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HBI3022791.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28410..29888)
                     /gene="wzxC"
                     /locus_tag="K0W59_001716"
     CDS             complement(28410..29888)
                     /gene="wzxC"
                     /locus_tag="K0W59_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HBI3022792.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29890..31284)
                     /gene="wcaJ"
                     /locus_tag="K0W59_001717"
     CDS             complement(29890..31284)
                     /gene="wcaJ"
                     /locus_tag="K0W59_001717"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HBI3022793.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLAHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGGNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31339..32709)
                     /gene="cpsG"
                     /locus_tag="K0W59_001718"
                     /gene_synonym="manB"
     CDS             complement(31339..32709)
                     /gene="cpsG"
                     /locus_tag="K0W59_001718"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HBI3022794.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32814..34250)
                     /gene="cpsB"
                     /locus_tag="K0W59_001719"
     CDS             complement(32814..34250)
                     /gene="cpsB"
                     /locus_tag="K0W59_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HBI3022795.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADKDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34253..35476)
                     /gene="wcaI"
                     /locus_tag="K0W59_001720"
                     /pseudo
     CDS             complement(34253..35476)
                     /gene="wcaI"
                     /locus_tag="K0W59_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
     gene            complement(35473..35952)
                     /gene="gmm"
                     /locus_tag="K0W59_001721"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35473..35952)
                     /gene="gmm"
                     /locus_tag="K0W59_001721"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HBI3022796.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35952..36920)
                     /gene="fcl"
                     /locus_tag="K0W59_001722"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35952..36920)
                     /gene="fcl"
                     /locus_tag="K0W59_001722"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HBI3022797.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENSQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36923..38044)
                     /gene="gmd"
                     /locus_tag="K0W59_001723"
     CDS             complement(36923..38044)
                     /gene="gmd"
                     /locus_tag="K0W59_001723"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBI3022798.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38070..38618)
                     /gene="wcaF"
                     /locus_tag="K0W59_001724"
     CDS             complement(38070..38618)
                     /gene="wcaF"
                     /locus_tag="K0W59_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HBI3022799.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38634..>38755)
                     /locus_tag="K0W59_001725"
     CDS             complement(38634..>38755)
                     /locus_tag="K0W59_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HBI3022800.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10346 a         9697 c         8725 g         9987 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgag ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcaccatca tcctgactag tctttcaggc gatgtgtgct
      601 ggaagacatt cagatcttcc agtggtgcat gaacgcatga gaaagccccc ggaagatcac
      661 cttccggggg cttttttatt gcgcggttgt taacggttca gacaggttta aagaggaata
      721 agaaaatgac agacaacact cgtttacgca tagctatgca gaaatccggc cgtttaagtg
      781 atgactcacg cgaattactg gcgcgctgtg gcattaaaat caatcttcac acccagcgcc
      841 tgatcgcgat ggcagaaaac atgccgattg atattctgcg cgtgcgtgac gatgacattc
      901 ccggtctggt aatggacggc gtggtagacc ttgggattat cggcgaaaac gtgctggaag
      961 aagagctgct taaccgccgc gcccagggtg aagatccgcg ctactttacc ctgcgtcgtc
     1021 tggatttcgg cggctgccgc ctttcgctgg caacgccggt tgatgaagcc tgggacggtc
     1081 cgctctcctt aaacggtaaa cgtatcgcca cctcttatcc tcacctgctc aagcgttatc
     1141 tcgaccagaa aggtatctct tttaaatcct gcttactgaa cggttctgtc gaagttgcgc
     1201 cgcgtgccgg gctggcggat gcgatttgcg atctggtttc caccggtgcc acgctggaag
     1261 ctaacggcct gcgcgaagtc gaagttatct accgctcgaa agcctgcctg atccagcgcg
     1321 atggcgaaat ggaagaatct aaacagcaac tgatcgacaa gctactgacc cgtattcagg
     1381 gtgtgatcca ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc gaacgcctag
     1441 atgaagtcat cgccctgctg ccaggtgccg aacgcccaac cattctgccg ctggcgggtg
     1501 atcaacagcg cgtagcgatg cacatggtga gtagcgaaac cctgttctgg gaaacgatgg
     1561 aaaaactgaa agcactgggt gccagttcaa tcctggtcct gccgattgag aagatgatgg
     1621 agtgatcgcc atgagcttta acacaatcat tgactggaat agctgtactg cggagcaaca
     1681 acgccagctg ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc gcaccgttaa
     1741 cgatattctc gataacgtga aaacgcgtgg cgatgaggcc ctgcgtgaat acagcgcgaa
     1801 gtttgataaa accacggtta ccgcgctgaa ggtgtctgct gatgagatcg ccgccgccag
     1861 cgaacgcctg agcgacgagc taaaacaggc gatggcggtg gcagtaaaga gtattgaaac
     1921 cttccacact gcgcaaaaac tgccgccggt agatgtagaa acgcagccag gcgtgcgttg
     1981 ccagcaggtc acgcgcccgg tggcttcagt gggtttgtat attccgggcg gctccgcccc
     2041 gctcttctca acggtattaa tgctggcaac tccggcgcgt attgcgggct gtaaaaaagt
     2101 ggtgctgtgc tcaccgccgc cgattgccga tgaaattctt tatgcggcgc agctgtgcga
     2161 tgtgcaggac gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg cgtttggtac
     2221 ggaatctgtg ccgaaagtgg acaaaatctt cgggccgggt aacgcctttg tcaccgaagc
     2281 aaaacgccag gtaagccagc gtctggacgg tgcggcgatc gatatgcccg caggcccgtc
     2341 ggaagtgctg gtgattgctg acagcggcgc tacgccggat tttgtggctt ctgatttgct
     2401 ttctcaggct gaacacggcc ctgactcaca ggtaatttta ctgacccccg atgctgatat
     2461 ggcgcgtcgc gttgccgagg ccgtcgaacg ccaactggca gaactgccgc gtgccgaaac
     2521 cacccgccag gcactgaacg ccagccgcct gatcgtgact aaagatttag cgcagtgcgt
     2581 ggagatctcc aaccagtacg gcccggagca cctgatcatt cagacccgca acgcccgcga
     2641 actggtcgat ggcatcacca gcgccggttc ggtatttctt ggtgactggt caccggaatc
     2701 ggcaggcgac tacgcctccg gcaccaacca cgtgctgccg acttacggtt acaccgccac
     2761 ctgttccagc ctcgggctgg cagatttcca gaaacgcatg accgtacagg aactgtcgaa
     2821 agaaggtttc tccgcgctgg cttcgaccat tgaaacactg gccgccgccg agcgcctgac
     2881 cgcccacaaa aatgccgtta ctttgcgtgt taacgtcctt aaggagcaag catgagcacc
     2941 gtgactatta ctgatttagc gcgtgaaaac gtccgcaacc tgacgccgta tcagtcggcg
     3001 cgtcgtctgg gcggtaacgg cgacgtctgg ctgaacgcca acgaatatcc cacagccgtg
     3061 gagtttaagc ttactcagca aacgctcaac cgctacccgg aatgccagcc aaaagcggtg
     3121 attgaaaatt acgcgcaata tgcaggcgta aaaccggaac aggtgctggt cagccgtggt
     3181 gcggacgaag gtattgaact gctgatccgc gctttttgcg aaccgggtaa agacgccatc
     3241 ctctactgcc cgccaacgta cggcatgtac agcgtcagcg ctgaaaccat tggtgtcgaa
     3301 tgccgcacag tgccgacgct ggaaaactgg caactggact tacagggcat ttccgacaag
     3361 ctggacggcg taaaagtggt ctatgtttgc agccccaaca accccaccgg gcaactgatc
     3421 aatccgcagg attttcgcac cctgctggag ctaacccgcg gtaaggcgat tgtggttgcc
     3481 gatgaagctt atatcgagtt ttgcccgcag gcatcgttag ctggctggct ggcggaatat
     3541 ccgcacctgg cgattttgcg taccctttcg aaagccttcg cgttggcggg gcttcgttgc
     3601 ggatttacgc tggcaaacga agaagtcatc aacctgctga tgaaagtgat ccccccctac
     3661 ccgctctcga cgccggttgc cgacattgcc gcacaggcgt taagcccgca gggaatcgtc
     3721 gccatgcgcg aacgggtggc gcaaattatt acagaacgcg aatacctgat tgccgcactg
     3781 aaagagatcc cctgcgtgga gcaggttttc gactctgaaa ccaactacat tctggcgcgc
     3841 tttaaagcct ccagtgcagt gtttaaatct ttgtgggatc agggcattat cttacgtgat
     3901 cagaataaac aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac ccgtgaagaa
     3961 agccagcgcg tcattgacgc cttacgtgcg gagcaagttt gatgagtcag aagtatcttt
     4021 ttatcgatcg cgatggaacc ctgattagcg aaccgccgag tgattttcag gtggaccgtt
     4081 ttgacaaact cgcctttgaa ccgggcgtga tcccggaatt gctgaagctg caaaaagagg
     4141 gctacaagct ggtgatgatc actaatcagg atggtctggg aacacaaagt ttcccgcaag
     4201 cggattttga tggcccgcac aacctgatga tgcagatctt cacctcgcaa ggcgtgcagt
     4261 ttgatgaagt gctgatttgt ccgcacctgc ccgccgatga gtgcgactgc cgtaagccga
     4321 aagtaaaact ggtggagggt tatctggctg agcaagcgat ggatcgtgcc aacagttatg
     4381 taattggcga tcgcgcgacc gacattcaac tggcggaaaa catgggcatt aatggtttac
     4441 gctacgaccg cgaaaccctg aactggccaa tgattggcga gcaactcact aaacgagacc
     4501 gttacgccca tgtagtgcgc aacaccaaag agacgcagat tgacgtccag gtgtggctgg
     4561 atcgcgaagg tggcagcaag attaacaccg gcgttggctt ctttgatcac atgctggatc
     4621 agatcgccac ccacggcggt ttccgcatgg aaatcaatgt caaaggcgac ctctatatcg
     4681 acgatcacca caccgtcgaa gataccggcc tggcgctggg cgaagcgtta aaaattgccc
     4741 tcggcgacaa acgcggtatt tgccgctttg gttttgtgct gccgatggac gaatgccttg
     4801 cccgctgcgc actggatatc tctggtcgcc cgcacctgga atataaagcc gagtttacct
     4861 accagcgtgt gggcgatctc agcactgaaa tgatcgaaca cttcttccgt tcgctctctt
     4921 acaccatggg cgtgacacta cacctgaaaa ccaaaggtaa aaacgatcat caccgtgtag
     4981 aaagcctgtt caaagccttt ggtcgtaccc tgcgccaggc catccgcgtc gaaggcgaca
     5041 ccctgccctc gtcgaaagga gtgctgtaat gaacgtggtg atccttgata ctggctgcgc
     5101 caacctgaac tctgtgaagt ctgccattgc gcgccacggt tatgaaccca aagtcagccg
     5161 tgacccggac attgtgttgc tggccgataa actgttttta cccggcgtcg gcaccgcaca
     5221 agcagcgatg gatcaggtgc gtgagcgcga gctgtttgat ctcatcaaag cctgtaccca
     5281 accggtgctg ggcatctgct tagggatgca actcctggga cgacgcagcg aagagagcaa
     5341 tggcgtcgac ttgctgggca tcatcgacga agacgtgccg aaaatgaccg actttggtct
     5401 gccactgcca catatgggct ggaaccgcgt ttatccgcag gcaggcaacc gcctgtttca
     5461 ggggattgaa gacggcgcgt acttttactt tgttcacagc tacgcaatgc cagtcaatcc
     5521 gtggaccatc gcccagtgta attacggcga accgttcacc gcggcggtac aaaaagataa
     5581 cttctacggc gtgcagttcc acccggagcg ttctggtgcc gctggcgcta agttgctgaa
     5641 aaacttcctg gagatgtgat gattattccg gcattagatt taatcgacgg caccgtggtg
     5701 cgtctccatc agggcgatta cggcaaacaa cgcgattacg gtaacgaccc gctgccgcgc
     5761 ttacaggatt acgctgcgca aggtgccgaa gtgttgcacc tggtggatct gaccggggca
     5821 aaagatccgg ctaagcgtca aatcccgctg attaaaaccc tggtcgcggg cgttaacgtc
     5881 ccggtgcagg ttggcggcgg tgttcgtagt gaagaagacg tggcggcgtt actggaagct
     5941 ggcgttgcgc gcgtggtggt cggttccacc gcagtgaaat caccggacgt agtgaaaggc
     6001 tggtttgaac gtttcggtgc cgatgcgctg gtgctggcac tggatgtccg tattgacgag
     6061 caaggcaaca agcaagtggc agtcagcggc tggcaagaga actcgggcat ttcactggaa
     6121 caactggtgg aaacctatct gcccgtcggc ctgaaacatg tgttgtgtac cgatatctcg
     6181 cgcgacggca cgctggcagg atctaacgtc tctttatatg aagaagtgtg tgccagatat
     6241 ccgcaagtgg cgtttcagtc ctccggcggc attggcgaca ttaacgatgt tgctgcccta
     6301 cgtggcactg gcgtacgcgg cgtaatagtt ggtcgtgcat tactggaagg taaattcacc
     6361 gtgaaggagg ccatcgcatg ctggcaaaac gcataatccc gtgtctcgac gttcgtgatg
     6421 gtcaggtggt gaaaggcgta cagtttcgca accatgaaat cattggcgat atcgtgccac
     6481 tggcaaaacg ctacgccgaa gaaggtgcag atgaactggt gttctacgat atcaccgctt
     6541 ccagcgatgg ccgtgtggta gataaaagct gggtatctcg cgtggcggaa gtgatcgaca
     6601 ttccgttttg tgtggcgggt gggattaagt ctctggaaga tgccgcgaaa attctttcct
     6661 ttggcgcgga taaaatttcc atcaactccc ctgcgctggc agacccaaca ttaattactc
     6721 gcctggccga tcgctttggc gtgcagtgta ttgtggttgg tattgatacc tggtacgacg
     6781 ccgaaaccgg taaatatcat gtaaatcaat ataccggcga tgaaagccgc acccgcgtca
     6841 ctcaatggga aacgctcgac tgggtagagg aggtgcaaaa acgcggcgcc ggagaaatcg
     6901 tcctcaacat gatgaatcag gacggcgtgt gtaacggtta cgacctcaag cagctgaaaa
     6961 aagtgcgtga agtttgccac gtcccgctga ttgcctccgg tggcgcgggc accatggaac
     7021 acttcctgga agccttccgc gatgccgacg ttgacggtgc gctggcagct tccgtattcc
     7081 acaaacaaat aatcaatatt ggcgaattaa aagcgtacct ggcaacacag ggcgtggaga
     7141 tcaggatatg ttaacagaac aacaacgtcg cgaactggac tgggaaaaaa ccgacggtct
     7201 gatgccggtg attgtgcaac acgcggtatc tggcgaagtg ttaatgctgg gctacatgaa
     7261 cccggaagcc ttagacaaaa ccatcgaaag cggtaaagtc accttcttct cgcgcactaa
     7321 acagcgactg tggaccaaag gcgaaacctc gggcaatttt ctcaatgtgg taagtattgc
     7381 cccggactgc gacaacgaca cattactggt gctggcgaat cccatcgggc caacctgcca
     7441 caaaggcacc agtagctgct tcggcaacac cgctcaccag tggctgttcc tgtatcaact
     7501 ggaacaactg ctcgccgaac gtaaatatgc cgacccggaa acctcctaca ccgccaaact
     7561 gtatgccagc ggcactaaac gcattgcgca gaaagtgggc gaagaaggcg tggaaaccgc
     7621 actggcagca acggtacatg atcgctttga gctgaccaac gaggcatctg acttgatgta
     7681 tcacctgctg gtgctgttgc aggatcagga tctggattta acgacagtaa ttgagaacct
     7741 gcataaacgg catcagtgag ttacgtgctg agcaggtgtg atgttgttgt cacacccgga
     7801 gccattgccc gatgcgcaaa cttatcgtgc ctacacatta tctcaacaca cgtaggccgg
     7861 ataagatgcg ccagcatcgc atccggcaaa aaaaacgggc atggtgtcac caccctgccc
     7921 gtttctctta aatgcacaat aatattactt cgcgttgtaa ttacgcagag cgttacgccc
     7981 caacacaatc cccgcgccaa ccatgccgcc cagaagcact gccagaatca aggtgattgc
     8041 ctttttcgga ctatcgcgac gaataggtaa cgtcggtttc atcacatagc ggtaagcatg
     8101 aatatcaaga tcatcaaact ttaatttttc aatatccaac aggttttgac gtgtctgata
     8161 gtagtttggt gagaacacca acggacgagt cgcttcatgc ttaatcatcg actccagcgc
     8221 ttcgctccct agaaggaaca acgtatcttg cgtcacatct tcagtctgct gaacctgtgg
     8281 ctttgtcacc tgcgcctgat tcgcatactg caacgcttcc tgaatctgac ggatacgcag
     8341 atctttctgc tcctgcgcga ccacttcctg ggttcttaaa gagtcctgca agtttttccg
     8401 tcccagagca atgttgtcct tgagatcctt ttctagctct tgattcactt tatcatcaac
     8461 ttgctgaatg tattgggcca acttcatttg tgcgccctct gcagtttgcc caacataaga
     8521 aacagtcaat ggtaattgtt ggttcttaac agaaggttcg atggtaagtt tttctggctc
     8581 ttcctgatta tccagcgttt ctgctaatgc agagaaggca gaactgaagc gaccaattaa
     8641 cgtctcctgc aaatccgata ctttcggtgc agcctgacca tagataacat tcatggcatt
     8701 gttatagcca gcaatttgcc ccacgtcggg ctgagtgata attgctgttg acgtccattt
     8761 ctccttcgct actgccaaat aaccaatagc cagggcaata gccacaatga cggaaatgat
     8821 aattgtcatc ttgccacgcc acaactgcac tagtaaatca atcaaatcaa tctgttccgg
     8881 gtcatggttt tgcccagaaa cattattatt ttctactctc atcattaccc taactgacga
     8941 aaatttccaa gtccgcatag tttagcgata atccccgtga atgctatagg ataagtgata
     9001 aaaataatga attattgcgg aggtaacaaa agacaatcag ggcgtaaata gccctgataa
     9061 caagatgtta gtcgctgcca aagagatcgc gggtgtagac cttatctgcc acatccttaa
     9121 gctcttctgc catacggttg gagataatga cgtcggcttg ttgtttgaag gtggcgagat
     9181 cacgttccag gcgagagttg aagaatgagt cttctttcat caccggctcg tagatgatca
     9241 cttcaacgcc tttcgccttg atacgcttca taatcccctg aatagaagac gtacggaagt
     9301 tatctgaccc gctcttcata atcagacgat aaatacccac cacttgcggt ttgcgtgaca
     9361 aaatggcatc ggcaataaaa tctttacgcg tgcggtttgc atcgacaatt gccgagatca
     9421 ggttattcgg cacagactgg tagttcgcca gtaactgctt ggtatctttc ggcagacaat
     9481 aaccaccata accaaacgac ggattgttgt aatggttgcc aatacgcgga tcgagacaaa
     9541 cgccttcgat aatctggcga gtattcagac ctaaactttc tgcataacta tccagttcat
     9601 taaagtatgc tacgcgcatc gccagatagg tattagcgaa aagtttaatc gcttctgctt
     9661 cagtggagtc ggtaaacagg gtcgggatat tttgcttaat cgcgccttcc tgtaacaacg
     9721 cagcaaaacg ttctgcgcgt tctgaacgct caccgatgac aatacgtgac ggatggagat
     9781 tatcgtaaag ggctttacct tcgcggagga attccggaga gaaaataata ttttcagtac
     9841 gatatttctt atgcatcgct gcggtaaaac caacgggaac cgttgatttg atgaccataa
     9901 ccgcataagg atttatctca actacgtctt taattactga ttctacactg gatgtattga
     9961 aataattagt tttaggatca tagtcggttg gagtggcgat gatgacataa tcagcatccc
    10021 ggtaggcttc atttttatct aatgtggcat taaagtgtat tttatctgat tgcaaaaact
    10081 gctgaatttc cttatcaaca ataggagata tccgatcatt cagcatagca acgcgtgacg
    10141 gtaaaatatc taatgccaca acctcatgat tttgtgcgat tagaagcccg tttgacaagc
    10201 ctacatagct agtaccggaa atggtgattt tcatttcatg ctctcagaat taacttaact
    10261 gtgaatcatg atgtttttag catcgtgata agatgctaaa agttttaacg ctacgttgtc
    10321 agatggtcaa cgcaacgaaa aacaattcag attttcttta taagaatagc tgatgtttat
    10381 taagttaata ttaagcagtg aaatttagtc tataagatat ttggcaaaaa aaagcccggt
    10441 gcaatatacg ccgggcctca attttattgt tggttaaatc agattaatcc agccattcag
    10501 tatggaacac accttcttta tcaatgcgct tataagtatg cgccccgaaa tagtcacgct
    10561 gtgcctggat caggttcgca ggcagaaccg cggcacggta gctatcgtaa taggcaaccg
    10621 cagcggcgaa ggtcggaacc gggataccgt tctgtactgc ataagcgacg acatcgcgca
    10681 gcgcctgctg gtagtcatcg gcaatttgct tgaagtacgg ggccagcagc aggttagcga
    10741 tctgcggatt ttcggcataa gcatcggtga ttttctgcag gaactgcgca cggatgatgc
    10801 agccagcacg gaaaatcttc gcgatttcgc cgtagttcag atcccagttg tattcttcag
    10861 acgcagcacg cagctgagag aagccctgag cgtaagaaac gattttgccc agatacagcg
    10921 cactgcgaac tttttcgatg aactcagcct tgtcgcctgc tgactgagct tgcggaccag
    10981 agagaacttt agatgcggca acgcgctgat ctttcagaga agagatataa cgtgcaaaca
    11041 cagactcggt aatcagcgac agcggttcgc cgagatccag cgcgctctgg ctggtccatt
    11101 taccggtgcc tttgtttgct gcttcatcca gaatcacatc aaccaggtag ttaccgtctt
    11161 catctttttt ggtgaagata tctttagtga tgtcgatcag gtagctgcta agttcaccgt
    11221 tattccactc ggtgaaagtc tgggccagtt cttcattaga tagattcagg ccacctttga
    11281 gcagagaata cgcttcagca atgagctgca tatctccgta ttcaatacca ttgtgaacca
    11341 tcttcacata atggcctgcg ccgtcggcac caatataagt aacgcatggc tcgccgtctt
    11401 ctgccacggc agcgatttta gtcaggatag gcgcaaccag ctcataagcc tctttctgcc
    11461 cgccaggcat aatagaagga cctttcagcg cgccctcttc accgccggaa acaccggtac
    11521 cgatgaagtt aaagccctct gctgaaagct cacgattacg acgaatggtg tcctggaaga
    11581 aggtattacc accatcaatg atgatgtcac ctttatcgag gtatggtttc agggaatcaa
    11641 tcgcagcatc cgtgcctgca cctgctttca ccattaacag gatgcgacga ggcgtttcca
    11701 gagattcaac aaactctttc accgtatagt aaggagccag tttcttgcct ggattttcag
    11761 caatcacttc ttccgtcttt tcgcgggagc ggttgaaaat agagacggta taaccacgac
    11821 tttcgatgtt gagcgcaagg ttgcgcccca tcactgccat accgactacg ccgatctgtt
    11881 gctttgacat tgtttactcc tgtcttctat attattaacc gtctattaac cgttatatta
    11941 ttaaccgtcg tgattttcat cttctaatca aggaatacat atggttacag catgcttctt
    12001 actttatagc gataagtatg ccattgggtg tatccataag aactttgaat tttggatact
    12061 tttctggatt aaaattagca tagcttcgtt tagatataac aataaacgaa ctattaaaag
    12121 atttcatctt ctctattaaa tcatcctgtg ggattacctc acactcggga ttactaatag
    12181 tccaatcact atatcttgct aatccaaaga cgtcaccaat tgccatccct ttatagaagt
    12241 aaataccatc ctccaagcct aattggataa gattatctcc atataactca ctatattcac
    12301 ttgctttctt catcagttca tagctatagc gactttctaa gcgtgaatta aaattcaagt
    12361 attcagaata aaaatatttg aattcaaaat gcaacgatcg ccccgaaaaa aaaataatac
    12421 acacaaggat aaaatatcgt cccaacttgt aatttgtaat gttgatatct tttattaaac
    12481 ttgcaattga gatcgcaacc agcaatactg acaatgggta aatgggtgat aaatacctgt
    12541 caccttgcgt aacagaaaac caaaataaga catatgttaa aacaatcgag aaaaataccc
    12601 aaagtatttt attattccta agtgtaaaga cagacagtat agcataaaaa acaacatcac
    12661 tgccaacttt attaattgct tgttgcaaat ctaatgtggc aggacttata ccatgtcgag
    12721 cttgttctgc tgtttgaact tgtagatcaa tttcattcca taggtagtga ccaaatatat
    12781 ttcctccagc aggactaaaa ggatcaccaa agatccagaa actacggaca taccaataaa
    12841 tgccaccaag aattgttgcc aagctaaaaa taagaaatgt ctgtatgttt ctctttttcg
    12901 ctatcaatat aacaaatatg atggctccat atatggcacc aaatagtttt acgccacaag
    12961 caattccaat aaaaaaacca cataaaaaaa ttaattcatt ttgtgtttta ttgttttctg
    13021 agcgaatata tataagaata ctggctgcag caaaacaaag atagcgttaa atcaatatat
    13081 gcataaccaa gatgatcttt aagtggattg atagtaaata gtaggattgt agcaataatg
    13141 ctaggataat aagttttcgt aatttcccat gaacagccaa caagtccaca ggctattaca
    13201 aaaaaacaga tatttgctaa aaactgagct aaccttacat ctcctaactg taaacctaaa
    13261 gccattaaaa gatctgcatt ttgcgggaac agaggaaaac gcaaatattg ttcaacaaca
    13321 attgcatggt gctcaactaa tgaacgagcc aaaggtaagt gatacatagt atcatcccaa
    13381 tcaccgggag ctcgcaggga cgataacaat gtaaccacaa aaatcaaaac aagaaataaa
    13441 ataacaaaca gcgtactgaa ctcaatatta tttattgcca gccatctaaa ttgctgacgt
    13501 ttaataatgc ttaaaaccca acacaccgta aaaattaaaa agatagcaat cttattaaat
    13561 tgcccgacca tgcccagaaa gtaaattatc aaaatattta aagacatccc tatgcatagg
    13621 caataaaaaa ataaagaaaa tttgttaagt cctgccaccc cctccttaaa aaaagatgac
    13681 aatacaatat taccaatgac aaaaaatgtc attccaaaag caaaacaata cacaacatac
    13741 aaatacaacg gcaattcaat catagtaaaa tactctattt acattatcag tttatagttt
    13801 ttttcagaaa gataaaataa tgccttcatt tcacgtctag ttgttgtatt tgaatgcaaa
    13861 ataatgccac atacaaatgc cattattgac gataacatga tggaggcagt taaaaaggca
    13921 gtcggaaatc ttctcaccaa acctgtatca atataatcaa caatgatggg caatcctaaa
    13981 attatagaca aagcagcaag taaaacgccg ataatactaa aaaagtatag tggccgttca
    14041 agcgagtaaa gtttaattat tgttttcaga atcttgaacc catcactcca agttgatagt
    14101 ttgctcacac tcccttcaga tcgctcgcca tattttgtat taacctcacc atacttcata
    14161 cgtaattcta aagcatgaat tgtaagctca gtttcgattt caaatcccct tgatagacat
    14221 ggaaaagatt taacatagcg tctggaaaat acacgatacc ccgacaacat atcagtaaaa
    14281 ttccctttaa agatagtctg aacagtacca gtcaacagtt tatttcctca gcgatgccct
    14341 tttctgtatg tattttgatc tccattctct tgacgacatc ctacaaccat atccaattga
    14401 ttttttatga gatggttaat tatttctgga gcagaacttg catcataagt gccatctcca
    14461 tcaaccatta gataaatatc cgcatctaca tctgagaaca tacgtcttac gacattcccc
    14521 ttgcctttca gaggaacaga gtgaactgtt gctccagcat cctctgccac ttgtgctgta
    14581 ctgtcagtcg aattattatc aaatacatat atagaagcag ttggaatatt cgttttgaaa
    14641 tcatttatga cttttgctat tgccttctgc tcattataac aaggaataat tacagcgata
    14701 ttaaaagagt gatacataag cccctcatat aaactattta gctaatgtgc aagtaattgt
    14761 gtttttaaca aatcgtttac taacgaaaaa tgaaatattt tgatgcgcaa tatcctaaaa
    14821 ccaaactaaa aagagaaaag gtgaacaatg taaatattgg agataaagct aagagatcaa
    14881 ataaaacgcc gaataaataa ctcatcgccc ccataaaaaa tataaatata aaatatctag
    14941 ccttggttgc agatacttta aatgagcatc ttgcatttgc aaaaaaagaa aaggttgctg
    15001 cacatataaa accaacaatg ttacttcggc cttgaccaaa agctaaatta ttataacacg
    15061 cataaaatac cccccaatgt aaagcagtat tgacaattcc aattaaaaaa taactaaaaa
    15121 aagaactggt caataacttc cctattttta acatccttta ttttgctcca gaagtgaggt
    15181 tattactaat ttggatattt tctatagaaa atacccataa aactacaacg cctataataa
    15241 aaattcccat gggtgtaaaa agaaatggat tagttataga aacggtaata ctgaaaaaaa
    15301 gtattaacga catcattaat gatgtttttg tactatttaa aaaatagcct ttcaaaaaga
    15361 taagcaacat aggaactaat gatataaata caccaataac gcctgttttc tgaagtatct
    15421 caagaggtac tatttcaatc cttaatcgat ctaatataaa tgaaccaaat cctcttccaa
    15481 acaagaacgt cgctaaatca acatttttat aataaaaata taaatcatta aatctaacag
    15541 agtcagaatc ttcactgcgt gatccgacca tgtaaagcat aattataaat agaacgctgc
    15601 ccaatattat tataatagta tttaaatata gcttatgtaa tttaaaactg gctaataaca
    15661 gtgataatgt tgtaaaaagt aatacacctc ttgattctgt caataatatc gcaagcacta
    15721 ttatgagaca ttttaaatat tttttgtcga caaaactgat aatcgcaccg acaccaaaaa
    15781 agtagaagga tttggaaaaa aaagccccat cgggacgaaa aaagaactcg ctagtcaatg
    15841 ataagtgttc ataaattaaa ttaaaattta acaaaccgaa attcaacaaa attatgtatg
    15901 aaatatataa cagtgatcca tataatgcaa atgcattaac tattttaata aaaatctcac
    15961 ataaaaatac ttttaaagta tatcttagtg taaatagata tataagtgac agcataaaaa
    16021 ggtatggttg cacatcaaac agtatatcat ttattttatt accagctaat aagccgacaa
    16081 agaagccata aaaaggaaat aaaagcaacc atataaaaag actgacacat ttttgggtga
    16141 ttgaaaaatt gaatgtaact aaattaaaaa ttaaaagtat aaaactaaat acaaataata
    16201 aatatcgcaa agggaatatt cccaatggct ccagtaatct tccaccacca ccaataatca
    16261 gttcaataca taaaaatgtt ataaaaattt tatttatatt attcatattc gtaaggtgat
    16321 gttttttaaa atatgtttaa aatcaacatt aataaaatat aaattttatc agcctttttc
    16381 cgccttaact tccaaaaact taaagccaag atcaaattgc ctatataacc tttacccaat
    16441 tgattattca caaagctatc atcagctaag gcattagcta ttttagttac ttcttttcga
    16501 taccacttgt ttctcaccaa accaagccgt ttgtattttg ctttaaaact aatgtttgct
    16561 cccacttggt tgttttcatg ctgtctgtac agcattgttg gttttctatc aatataccac
    16621 gaataatttc tcgtccgcgc aaatgaatat aaaaaccaat catgcagaca gatatcttgt
    16681 gcagcatttt tattatttat gatgaacttc ttaaattcaa taagcgtttc tttttttata
    16741 acatatgtac accccggacc tgctgcttca aaaaaataat cgaattgagt ttgcgactga
    16801 gccttatcaa ctaaattttt ttgccctgat ggataatatg caattacatt gctggaataa
    16861 caattcgcat tattaaatac caagcagtca attgcatttt ttattttatc tttataccaa
    16921 atatcgtcct gatcactaaa acagatataa tcatagtttt caagatcgac atctttaatt
    16981 aatctataaa aatttttacc tgccccccca aacttattga cgcttggaag tatatttata
    17041 attgagggat atgataattg aaattcttca caaatattta atgtattatc agttgataaa
    17101 tcatctgata taaaaattga gatgtctaca tctttttgtt caataatact ttttaattgt
    17161 tcctcaatcc agcattcgcc attatacgta gccataagta cagctactct tttttgatca
    17221 gaactatgca ttataacgac cgcccccagt aattcctctt attccatcca gcgtagccaa
    17281 aatatgtttt ttcatatatt caattttatt tttttgagtt gcgataatta aagtcgtatg
    17341 aattaaagaa aaaacaactt ccctaataga gagtaaaaga ggcacatgat tttttctaag
    17401 caaaagaaat gcatttctta ctcgataata acgacgtact ggcgagtgaa ttggtatgtt
    17461 aatcccacag atttttactg acttatcccc aatagcatga accatttcta tatcaggaat
    17521 gatatgaact aatatgccat agtttaagca tctaagacac cattctgtat cgacataatc
    17581 aataaaaagt tcctcatcca tcattccaac aatctcaaaa acttcttttg aaaccatggt
    17641 tccggatgag attgttactg aacttttaaa cggtgtctgt ccttcagtga catgaatttt
    17701 ctctcgaaga ccattttttt tgatattaca aataggataa taatgtgact tatctctatc
    17761 aataaaaact gggccagcta tttttgcatt ttcattatcc atatagcagg aaagtttttt
    17821 gataaactca ttgcgaatac ggctatcttg atcaaaaaaa attatcttct ctgccccttt
    17881 ttcaagagaa tatcgaatcc caatattttg tgcttttgct ataccttcat tcctgccaag
    17941 acaaattaaa tgaacttttt caatgtctgc taatttatct ttcaaggtga aattagtcct
    18001 attatcgact actacaacgt aacaattttg ctcctttagt tcagtgatca gtgcattcag
    18061 atttttaagt tcgggattat atgtcacaat gacagcgtaa atattactat tcattatcca
    18121 agtgactcag taattggtta attttagttt tcatatacag aacacattct agtataaagc
    18181 cattgtaata acagagcatc taaaattaca cgtatgctcc aggatattgc ggcacccagt
    18241 aacccatatt tttcgaccat aaaatacaaa agggctaaat aaggaaccaa ttcagcacaa
    18301 tgaatcaaag cagtaatttt agattttcct aatgcctgga ttgcagagaa tggtatcatt
    18361 gccagcgcat tgaagaaaaa accgatcaat aacacattta atatattaaa ggaattaatt
    18421 tggtaattct caccaaacca tattttcaat acaagccctg aatataacaa gcctatgatt
    18481 atcaccggca aacagattag aaacattaaa agcaatgatt tatttacatt acgtttaaat
    18541 tctttaaagt ctttgatgtt acttaacctt ggaaacactg ccctcccgat tgctgcaggt
    18601 attattccta attttagaat aacctccgct ggtgcagaat aaaatgcaac tttatctgcc
    18661 cccataatat ttgacactat aaatcgatca aaatacacca tgaccggact tatgatatta
    18721 cttactgtca tccaaccgcc aaagaaaaag agtcgtttaa aggttttata acaaaaatga
    18781 acccccgaaa ttttaatatc attcctgaca taataggcag agactaaaat cgaaataact
    18841 cttgcaaata ttaaaccagc aaccgctgcc gacaatgtag cactataaaa aacaaatatg
    18901 gccggaattc ccgcgatgca agagcttgat atagattttt gaatatttac aatgccaaat
    18961 ttttcatccc cctcaagaat ggctgaccat aattgattta gaataaataa tggaatgcaa
    19021 atagccaaca gtttaaatgc tagttgtata tcactatgtt caacaccaga aatttttaga
    19081 taattaacaa ttccatcaga gaaaatcaat aataaaaaag caccaaagca tgaaaatagc
    19141 accaaaaaag atgtacttgt tgaaattacc ttggttcttt catgatgatt atcacgatga
    19201 attgcaattt ctctaataac agagcgagtc aggcctacat caaaaattcc agcataccca
    19261 acaagtgcaa ttgcaattgt ataaacaccg aaaccttcag gccccaaacc tcgagcgaga
    19321 aatcctagcg aggggatagc aattaaagtt ggtattgcat agccaaaaag gttccagaca
    19381 ctatttttta ttatgctcat tattacattt ctttatcata taaaataatc agcatctaca
    19441 aatttttgac cattaatatc tttatcagaa agcaatggat tttgaaccgg ccattctata
    19501 tttatacttt tgtcgctcca taaaatccca cgctcttgca tttttgaata gaaattattt
    19561 gttttgtaga caaattctgc ttcatcacta agaacgcaaa acccatgagc gaatccttcc
    19621 ggtatccata attgacgctt attctccgcc gacaaattta caccaaccca cttgccaaat
    19681 gttggtgatg atttccgaat atcaacagct acgtcaaaaa cttcaccgac aacacagcgt
    19741 accagttttc cttgagcata aggttccagc tgatagtgca gtccgcgtaa cacaccctta
    19801 gttgatttag aatggttatc ctgaacaaac tcaacctttc gtcctacagc ctcttcgaaa
    19861 actttctgat tgaagctttc cataaaaaag ccccgctcat caccaaatac ttttggttca
    19921 aaaattagca catcaggaat ttcagtttta attacgttca ttttattaat aacctttaat
    19981 cattttcagc agatactgac cataagcatt tttcttcagc ggctcggcta ataccttcac
    20041 ttgctcagca tcaataaagc ctttacggta agcaatttct tccgggcagg aaactttcag
    20101 cccctgacgc tcttcaattg ttgcaatgaa gttgcttgct tcaataagac tttgatgtgt
    20161 ccccgtgtcc agccatgcat aaccacgccc catcatagcg acagacaaac gcccttggtc
    20221 catataaata cggttaatat cggtaatttc cagttcgcca cgtgcagatg gtttaaggtt
    20281 tttcgccatt tctacaacgt cattgtcata gaaataaagc ccagtaaccg catagttact
    20341 ttttggttcc agcggttttt cttccagact aattgccgta ccgttatcat caaattccac
    20401 cacaccgtag cgctcaggat catttacgtg ataggcaaaa accgttgcgc cactctctct
    20461 gttaacagcg gtatccatta atttcggcag gtcgtgacca tagaagatat tatcaccaag
    20521 taccagcgca caatcatcac cacctataaa ttcttcacca atgataaatg cttgcgccag
    20581 accatccgga ctcggttgta ctttatattg cagattaagc ccccactggc ttccatcacc
    20641 aagcaattgt tgaaaacgtg gtgtatcctg cggcgtactg ataatcagaa tatctcgaat
    20701 acccgccaac ataagcgttg aaagcggata ataaatcatc ggtttatcat aaatcggtag
    20761 cagttgtttg ctcacagcca ttgtcacagg ataaagacga gtgccggaac caccagccag
    20821 aataatacct ttacgcgttt tcatttcact attcctttta attcatctcg ctctggcatc
    20881 atgagcgaga tgcaataatt tgttaaattg ccgtggtcgt aaataattcg ttgagcatac
    20941 gtttcacgcc cacctgccag tcaggcaaga caagcgcaaa gttctgctga aatttttcgg
    21001 tattgaggcg agaattatgg ggacgacgtg ctggtgtggg ataggccgtt gtcggcacgg
    21061 cattaagttt gttaagagca aggttgatcc ccgctctgcg cgcttcttca aataccagag
    21121 cggcataatc gtgccaggtt gttgtaccac cagcaaccag atggtacaaa ccagcaactt
    21181 ctggtttatt tgcggccacg cgaatggcat gagcggtgca atcagccagt aattcagccc
    21241 ccgttggtgc gccgaattga tcgtttatta cagccaactc ttcgcgttct tttgccaggc
    21301 gcaacatcgt tttggcaaag ttatttcctt taccagcgta tacccagctg gtacggaaaa
    21361 taagatgctt tgcgcaatgt tcttgtaacg ctttttcccc agctagcttg gtttcaccgt
    21421 agacatttag cggtgcggtt gcatccgttt ccagccatgg cgtgtcaccg ttgcctggga
    21481 aaacataatc agtggagtag tgtataaccc aggctccgac ttcattagct gcttttgcaa
    21541 tcgcttcgac gcatgtcgcg ttaagtaatt gcgcgaaatt cggttctgat tccgctttgt
    21601 ctactgcagt gtgagcagca gcattaacaa taacatcggg gcgaattttt ttgacggttt
    21661 cagccacacc ttcggggttg ctgaaatctc cacaataatc agtggagtga acatcgagcg
    21721 caatcaagtt acccagagga gccagagcac gctgcagttc ccaacccacc tgccctgttt
    21781 tgccgaaaag caggatattc attactgacg gccctcatag ttttgttcaa tccacgactg
    21841 ataagcaccg cttttcacat tctcaaccca atttgtatta gccagatacc attccaccgt
    21901 tttacgaatc ccgctctcaa acgtttcttg cggtttccag cccaattcgc ggctaatttt
    21961 atcggcatca atggcgtaac ggcggtcatg ccccgggcga tcggcaacat aagtaatttg
    22021 ctcacgataa gatttctctt tcggtacaat ctcatccaac aaatcacaaa tagtgagcac
    22081 tacgtcgatg tttttctttt cgttatgtcc accaatgtta taagtttcac ccgctttacc
    22141 ttcggttacg acggtatata acgcacgagc atgatcctct acatacaacc agtcgcgaat
    22201 ttgatcccct ttaccataaa taggtaatgc cttaccttcc agagcattca gaataaccaa
    22261 tggaataagt ttttccggga agtgataagg accataattg ttcgagcaat tagtcacaat
    22321 ggttggtaaa ccataagtcc gcttccaggc acgtaccaaa tgatcgctgg aagctttaga
    22381 cgcagaatac gggctacttg gcgcgtaagc tgtcgtttcc gtaaatagcg gtaacgcttc
    22441 gttattattt gcttcatccg gatggggtaa gtcaccataa acttcatcag tagaaatatg
    22501 atgaaagcgg aaactttttt tcttttcatc attcagagca gaccagtaat tgcgcgccgc
    22561 ttctaaaaga acataagtcc ccacaatatt ggtttcaata aatgccgcag ggcccgttat
    22621 tgaacggtca acgtggctct ctgccgccag gtgcatcacc gcgtctggct ggtgctgtgc
    22681 gaaaatacgc gacatggcta cagcatcgca gatatctgca tgctcaaaag cataacgttc
    22741 agaatcagaa acatcagcga gtgattctag gttcccggcg tatgttaatt tatcgacatt
    22801 aacaacacta tcttgcgtat tatttattat gtgacgaaca acagcagaac caataaaccc
    22861 tgcgccacca gtaacaagta tcttcacttt tttattccat atagctatag agcatgctgt
    22921 gaaatagact gctccagatt tgattaatag aggtattaat acacgctacc gcccctggct
    22981 taacagctac cagtgcactg catgcatctc aacgatgtgg cgagcgttag ccactcgcgc
    23041 caaatccgaa aaattcaaac gttaattgtc ttaccaaaca gccgtagaaa caaggaaaat
    23101 cctggaaaaa tttgaataat atgagctatg taacttcttg tttttatagg ctttataaaa
    23161 acggcaacgg caggaaaatt caatgagcaa aacttatgcc acgaatcttc actgccgtta
    23221 taattttctt atcaaccgtt acatccggtc agattttcat tattcgctta acagcttctc
    23281 aataccttta cggaacttcg ccccttcttt caggttgcgt aggccatact tcacaaacgc
    23341 ctgcatatag cccatttttt tgccgcagtc gtaactgtcg ccggtcatca gcattgcatc
    23401 aacggattgt tttttcgcca gctcggcaat agcatcagtc agctgaatac gtccccatgc
    23461 accaggctga gtacgttcca gttccggcca aatatcggca gaaagcacat agcgacctac
    23521 ggccatgatg tctgagtcca gcgtctgcgg ctgatccggt ttttcgataa attcaacaat
    23581 acggctgact ttaccctcac ggtccagcgg ctctttagtc tggatgacgg agtattcaga
    23641 gaggtcaccc ggcatacgtt ttgccagcac ctggctgcgg cccgtttcgt tgaaacgtgc
    23701 aatcatggca gcaaggttgt aacgtagcgg gtcggcgctg gcatcgtcga tcacaacgtc
    23761 tggcagtacc acgacaaatg ggttgtcacc aatggcaggt cgcgcacaca aaatggagtg
    23821 gcctaaacct aaaggttcgc cctgacgcac gttcataatg gtcacgcccg gcggacagat
    23881 ggactgtact tccgccagca gttgacgctt cacgcgctgc tcaaggagtg actctaactc
    23941 ataagaggtg tcgaagtggt tttcgaccgc gttcttggac gcgtgagtta ccaggaggat
    24001 ttctttgatc cctgcagcca caatctcgtc aacaatgtac tgaatcattg gcttgtcgac
    24061 gattggtagc atctctttgg gtatcgcctt agtggcaggc aacatatgca tcccgagacc
    24121 cgctacagga ataactgctt ttaaattcgt cattatttca tccacctgta aaatggttgc
    24181 tgaattatag cttgttcgat ttttttcgcc agcatcaatt accctgaatt gattactgaa
    24241 ttacttgtga tgttacgccg cttcgttgtg gattgcagta gcattgttcc taagtatggc
    24301 tccatttttc caggaatggt cgcaaatcta ctccctccgt tccggcaatc taaagttaat
    24361 cttttccaca ttaacaatat ggtgattaat cctgtcgata tcgacggagc tttgccctct
    24421 ttcattcacc gcatgaacat ttgcaagaga cagcagtgtt tcttttttcg ccataaagac
    24481 gccgcgaacg tctttgcgca tgtcgaagtt catgctcaat gctggtccaa ctgaggattc
    24541 ctgcatcaca ttgatatttc gtataaaaag atgttgcggc ttgttgtgta actccagcga
    24601 ggcacgcttc atctcaatgt tagttagcga cacaaaggag acggcattcc cggcggagat
    24661 ttggatgccg cgcaatttat aagcaagatg ggtattatcc agttgaatat tattcactcg
    24721 gaaattttgc ggtatcgaga gatatttgcc tttaattacc ccatagccga ttaacatccc
    24781 ggcgctatta atcatttcaa tattatcaat cacgaaattg tcacaaccgt aaatagcgac
    24841 tgtcgcgtta tcaatgcccg ctttcttact gaaatccggc gtgatattgc gggcattgat
    24901 attacgaata acaaaatgtt taccattttc aacatggttc aactgccgac aatccgatcc
    24961 cgtgatattc gccacgacaa agtttttcac tgcctggtct tccgggtagt tgttatcata
    25021 agtgcttccc gcaaggccta tgccgatgcc ccagttgatt ttgccgttgg tacagttgat
    25081 acgctcgatg acatggtcag atatcaaaat atcactgtcg ttaattgcca cgttccattc
    25141 aatggcgtcg ccctgtaagt cgctgaactt acagttggta atgttggcac cgataatctg
    25201 gttatgaaat ccctggcgta agatggcgta attagcgtgg ctgacagtga ggttatcgat
    25261 ggtcaggttg cgcatgaccc gtttgttttt accgccaata taaatctgca ttaccgggcc
    25321 aaagccgctc atcgccagcc ctttgatggt gcagtcagaa ccacgcacat ccagggtaat
    25381 gttatgcata ccgccgccct ctccccctgt cacctggctg ccgtcctgta agacaaatcg
    25441 ccctctgccg ttgccgcgca ggcttccaag gatgtgtaac gttttgccgg gagggatgaa
    25501 gatgccggtg ttgatattgt cacaaacgaa tccggcaggc acgacgaccg tttgcccttc
    25561 gctgaaggct tgtttaaatg aggcgatcca gtcatgtggg ttgtagtcgt taatgttaac
    25621 gctttgtcgg gcgggaagtg cgcgagcgaa aggggtatgg aggaaggcaa gcgccgagct
    25681 tgccgtcagg aaggtgcgtc gggagagtgt tttaaatggc atacgttctc ctctataaag
    25741 cctgcagcaa gctggcgagt tctcgattaa tcacctgctg gttaaaatcg tgttcgactt
    25801 tttcgcgcgc acgtttgacg accggagcca gttcgtcggt atccagttgg ctaaacgccg
    25861 ccagacgttg tgccagtgcg cgagcatcgt tctcaggcac cagccagccg gatttatcgg
    25921 cctccaccag ttcaggaatt ccgctatgca gagtagaaac caccggaata ccgaccgcca
    25981 tcgcttccat tagcgccacc ggaatgcctt ccatatcgcc atccgcgccc gttaccgatg
    26041 gcaacaggaa gacatccgcg tcgtcgagca tctccttcac ttcatggctc ggtttaaagc
    26101 ccggcatctc taccacatct tccagttgat attgttcgat gagggtgcgc aggcgtcgtt
    26161 cccacaggcc aatgccgagg atgcgatagc gaaatgccac gccctgctct ttcaactgac
    26221 ggcaggcttc aatcgccaca tgcaggcctt ttttctcggt taagcgtgcg acagatatga
    26281 tttccagcgg cgttgcgggc gctttcaccg gacgcgggct aaagcgcgtc atgtccacgc
    26341 ccatacgcga tacggcgatt ttttctctgg ggcagcccat tttttgcagc cttccggccc
    26401 acagatcgct tatcggtaac atcaggtcgc cacggcgaaa cagttgttga tattcgggag
    26461 tgtagtggtt gagcacttcc cgactggaga tatcaatacc gtggaagatg gtggcaattt
    26521 tgccgcgaat gatacccagt tcgcgtagtt ttgctgcggt tacccccgcc gggccaaaat
    26581 gagcgatgaa cacatcggcg cgaaacggtg ttgcgacctg accacaaatg gcagacaaaa
    26641 tcaggttccg cgactcggca ccatagcgtt tgaggttgag cgcatgccag gtatttttac
    26701 gatgaatgcc acgtaaggtc tggctggcgc ggtggcgcag tttcgctact ttgcccgcag
    26761 gttcgtcctg taaccagcgg gttctggcga caaggttgta tttcgtccat gccgcgtggg
    26821 tgttctctgt atcgcctttt tgcaaagcga caatctccac ttcaaagccc atatcaataa
    26881 acgcggtaat ttgattgagg acgaaggttt ccgacgacag cgggaatttc aataagaaga
    26941 agccgacctt catttcacct ccccgatgcg ctcaagcaca gactgcacca tctgcattcc
    27001 cgtctgacgt tcgcgactga cggcttcgtt aaatcgagcg ttcagcaccg gaagctggcc
    27061 taaggtatcc gcaaccatcg cttgcaggct gccgtccagt aaatgacgga tatcaatcgc
    27121 catctctggc agtcccagct gctgcataat cccggcggat ttatgttcat agttgatggc
    27181 aatcgccgga gtagcaaagt tcattgagat aatggcggag tgcaggcgcg taccgacggt
    27241 gagttcacag gctccgagaa ttttgcccat ttccagatcg ttgagttcat ccatcactac
    27301 gtggtaacgg gcaggatcgc tgatgtgctg gcgcagattg agcgccacca tgcggtcgtc
    27361 tttgttatag ctgtcgatac ccgtacaggt ggagagcgca atcacctgat agccttcatc
    27421 gagaatgcga ttgaccaccc cggcaaaggc tttttcatac gcttgttgag tggtgccgag
    27481 acgtttgtcg aacggtgcca gttcgcgcag ggtaattgcc actgttttct gttgtgcggc
    27541 aacgtccagc cagtgttgaa cggcatagct ggcggtgaag tcttcggtgt ggtgatcgac
    27601 cagccacgcg gtatcgacgc catgttccac ttttgcggtg gtgatattgc tgcgtttcat
    27661 caaatcaagg ctgaccgatt cgcgcaggat cagcgcgtcg cagtgaccaa agacatagtt
    27721 cgccagttgg ttaaattgct catcctggaa ggggccgacg ctgtgaccaa tcataaacag
    27781 cggctttttc gccataaatg tgcaaagcgc atgttcaaac tgcggcacgc cgtagagatc
    27841 gacaaaaaac gatccgccga cctgaataat ggcgtcgtaa cctgacagca ggcgcacgaa
    27901 gtcggtgaat ccctgggcga tggcgatatt gcgcagcttg ccagtgtcgg tgacgcgtga
    27961 gagcaatacc tgatgctggt agcggcgacg gaggactttt ttaacgcgcc cgacaacgcc
    28021 cgccgcgcta ttgtgttgtg tcatctgcag gaacagcgga tcgcccatta ccgggcggtt
    28081 gagcagccag gaagaactga ccggatagcg gctcatcacg tccacttcgg tatgtggatt
    28141 gaggatgttg atggcatcaa gtaagccgcg caagatggcg ctgtcgccac gattgccgca
    28201 agtgtggttg cccagaataa gtaatttcat tttttcctca taaatttgat gccaggtgag
    28261 gctgtgtttg ttgtgttcgt tgccgttttt tgcctgatgc gacgctggcg cgtcttatca
    28321 ggcctacagg tcccgagcac agaaccgtag gacggataag gcgttttacg ccgcatccgg
    28381 caaccgttgt cggaaccgaa aacagcaatt caccccgccc gtaaaagcat tttcattttt
    28441 tcgctgcgac aaaactgacg cttcacttcc accaccagcg gatggcgtga cagcacaatc
    28501 atcaccacaa acgccagcac gcccgcggct atttgcaccg ccagcagcat ccccagcgcc
    28561 agttgccctt tcagcacaat gcccagcgca taactgacca ccagcgtcgg caacgagaga
    28621 taaaacggca gccacaaact caggatgtac tggcgataac tggaaccaag aaccggttta
    28681 atcatcacga agtaactcag aatggtgttg ataatctgca ccagcaggaa accgagcgtg
    28741 acgccgattg cgcccgccat ctgcccacca ataacaatcg ccggaataaa cagaaatgtt
    28801 ttgaatacat tgaatttaaa gctgatatcg acccgcgctt tcgccatcag cagcgaacca
    28861 atcgggttac ccacggagcg cagcagaccc acaatacaca gcaattgcag caccggaata
    28921 atgctgttcc acttttcgcc gaataccagc ggtacgaagt tattcgacac caccatcagc
    28981 cccagcagcg ccggaaagtt gataatcccc actatcgaca gcagcttgta gaagttaaca
    29041 cgcagctttt cggtgtcgtc ctgaattttg gcaaacgccg gaaacaacac gcgggtgatg
    29101 attgggttca gcttcatcgg cggtacgacg gccacgttgt acgccaggtt gtatccccct
    29161 gccacgcccg cgccaagaat acgcgccagc accagcgttg aaaggttggt gttgagatag
    29221 ttgatgatgc tgtccgccgt cagccaggca ccaaaacgta agttcggtgc cacagacgcc
    29281 agcgagaagt gcagaccggg gcaataaatt ttgcgtccaa agtagccaaa cagcagtgtt
    29341 ctcaccgcac tattgaccag ataaccgagg atcgcggtca tcgccagcgg ccagaaatgg
    29401 gcgctaacta ccgtaaaagt gaagcccgcc agcaccgcgc tggtttcgat catgccgatt
    29461 ttgttgaact ccagctcctt ctgcatcaac gcgcggaact gttgcccgtg ggggatcacc
    29521 acaaacgcca gcgacaatgt tttaatcaac ggtgccaggt ccgggttatt cagcacgtcg
    29581 ccgatgacat cactcaacaa aaacaccgcc acacatacca caagccccag cccgacgttc
    29641 agccagtaca gcgtggtgag ttcaaggtga ctgatttctt ttcgctgaat aatcgagtta
    29701 gcgataccga agtcagaaag cgtatctgcc agcgcgataa tcaccagcga cacagtcagc
    29761 aggccgaact ggtggttgtc gataatccgc gccagcacgg tcatctgcac cagcccgagg
    29821 ccgatgatga tcaccgtggc aatagccgac cacttcgcgc cgctgatggt tttttcacgt
    29881 aagctcatat caatatgccg ctttgttaac gaagcccttg aataccgtca ggaaaacgat
    29941 tttgatatcg aaccagacgc tccattcgcg gatgtactca aggtcgaact cgacgcgttt
    30001 ttccattttc tccagcgtgt cggtttcgcc gcgccagcca ttaatctgcg cccagccggt
    30061 aatgcccggt ttcactttat ggcgcagcat gtagccttca atgagctggc gatactgttc
    30121 gttatgcgct actgcgtgcg gacgtggacc gacaatcgac atccccccgg tcagcacatt
    30181 gataaactgc ggcaattcgt ccagcgaggt gcggcgcaga aagttcccca ctttagtgac
    30241 gcgcggatcg ttctgcgtcg cctgggtcac cactttgtcg ttctccatca ctttcatgga
    30301 acggaacttc cacactttga tcggcttgcc atccatacca tagcgagtct ggcggaaaat
    30361 aaccggccct ggtgaactga gtttcaccgc cagcgcaata cagcacagca ccggggagat
    30421 cagcagcaga ataagcgtcg ccagcacaat gtcttccgca cgttttagca ggcggttacc
    30481 ccccgaaagc ggcgtgtcat acagcggcac caccggaacg ccgttcatct cttcgagacg
    30541 tgaatggaga atgttgaagg taaagacgtc ggggatcagc agcaccgaac aggtggtatc
    30601 cgccagttga tgggccagtt ttttcactcg tgcgccgtcg cacatttgca tcgcgatata
    30661 gacgttatga atcttgcccg cttttgcatc ctcgaccagc tgttgcaggt tacccgccca
    30721 gtcgttagaa acgccgcccg gtttcgggtc gtggtaaacg cccaccactt caaaccctaa
    30781 ccacggctga ttacggaaac tctccatcag catttgtccg gcggctaaat cccccgctac
    30841 ggcgaccatc cgcttgttgt agccatgatt acgcagccag cccgccccaa tgcgaataca
    30901 tgaacggcaa accaccagtc cgatgctggt cagcccatac cacgccagcc agattttcag
    30961 ttgcgtgtcg aaatcattgt tgaacgccac cagtccggcg ctgaaaatca cgcttaaggt
    31021 ccagttttgt agcaacaggg caaattctgt cgctgcccga acaccgcgcc atgagcgata
    31081 aaaatcggtg atgccgccca gcatctggaa caccaccagc gtaatcagcg ccaccaacag
    31141 gtgcatgtag aggaatgaca gtccgctgac ttcgcaaacc agccatagcc cggcaaacat
    31201 gatggtgata tctgaaaagc gttgcaccat agagattaac gatgcattgg ttttcgctcg
    31261 ctcgcgcttt tttagatttg tcatcgttgt tcctgttatt agccccttac ccgcagtggg
    31321 gtaagggaag atccgacatt actcgttcag caacgtcagc agagttcgcg ttcgcgcttc
    31381 catcagcggc acatctccgc gcgattccac attcaggcgc accaccggtt cggtattgga
    31441 ggtgcgcagg ttaaagcgcc agtcggcaaa ggtcatgctg atgccatcgc tgcgatccac
    31501 cgccagcgcc tcacggctaa aatgctgttc cacgcggtta atcgcctcaa cgggttgcgc
    31561 cagtttgctg ttgatctcac cgcttgccgg aaacgccgcc atccggtcgc gcaccagttc
    31621 gcccagcgtt tttcctttca ggcacaccag ttcggcgacc agcagccacg ggatcatgcc
    31681 gctgtcgcag taagcgaaat cacggaagta atggtgggcg ctcatttcgc caccgtagat
    31741 ggcgtcttcc ttgcgcatcc gttctttaat aaaggcgtgt ccggttttcg acattaccgg
    31801 cgtgccgcct gcagcagtca ccacatcaat agtgttccag gagagacgcg gatcatggat
    31861 gatcttcgcg ccgggatttt tttcgaggaa cgcttctgcc agcaggccga caatgtagta
    31921 gccctcgata aactgccctt tttcgtcaaa cagaaaacag cggtcaaaat cgccatcaaa
    31981 ggcaatgccc atatccgcgc cgtgtttgat gaccgcattg cgggtgtcgt cgcgacattc
    32041 cggcagcagc gggttaggaa taccgttggg gaaattgccg tccggtgtgt tgtgtacttt
    32101 gattaattcc acgggcgcgc cgagggcttt aaagcgggct tcaatggcgt ccaccaccgg
    32161 acccgctgcg ccattcccgg agttgatcac cagcttgagc ggcgtgagat tcttgacatt
    32221 gatataaccg aacaggtgat caacgtaagc gtcacgcagg ttgatttgct gatagcgacc
    32281 gcgtttggtt tcatcgacgg gaggaaagtc gttggcttca gccagacgct ggacgtcgcg
    32341 cagtccggta tcaccgctga tcgggcgagc cccctcgcgc accagcttca tgccgttgta
    32401 atccatcgga ttatggctgg cagtaacttc aatgccgcca tccacgccga gatggaacgt
    32461 ggcgaaatag atctcttcgg tgccggacat accgatatcc agcacatcga cgcccgcatc
    32521 ctgtaaacct tttgccagcg ccagttttaa ggtttcgctg gtgagacgga catcaccgcc
    32581 taacacaatg gttttcggtt tgagaaattc gccataggcg cgaccaatgc gccaggcgat
    32641 atcttcattc agttcttcgc ctaatttccc gcgaatatca taggctttaa agcaggttaa
    32701 ttttttcatg tcgttaccct ttttcaggca atagttggcc ctgaccgaaa cggccaattt
    32761 tatttttgtc gttatttaca ccgcggtttc gcattcattg cctgatgcga cgtttacacc
    32821 cgtccgtagc gatccgcgaa gcgcaccaca tcatcctctt cgagataaga gccggagcgc
    32881 acttcaatta aatcgagcgg aattttcccc gggttttcca ggcagtgcgt cgcccccagc
    32941 ggaatataaa tggactcgtt ttcaccaagc agtttgatat caccatcaat ggtgactttt
    33001 gccgttcccg cgaccactac ccagtgttcc gcgcggtgat ggtgcatctg taccgacaaa
    33061 ccctcgcccg gtttcacggt gatgcgtttc acctggtagc ggtcgcccgc gtcgatagag
    33121 tcatatttgc cccacggacg atacacttcg cgatgcaccc gatgctcatg gcgaccatcg
    33181 gctttgatct gctcgaccac ttttttcaca tcctgtaccg cgttacggtc ggcaatcagc
    33241 accgcatctt tggtctgcac cactaccaga tctttcaccc cgacggtggt gactaggcct
    33301 gattcggcat acacatagct gttttcagtt ttgtgattaa tcacatcgcc gtggcaaacg
    33361 ttgccctcgg cggtgtgggc gctgatctcc cataatgaag accaggaacc aacatcgctc
    33421 cagcccgcat ccatcggcac cacaacagca tctgccgtac gttccatgac cgcgtaatcc
    33481 accgactctt ccgggcaggc gagaaacgct tcttcatcta cacgaataaa atcgagatcc
    33541 ggatcgacgg cgctcatcgc tttttcgcag gcatcgagga tatccgggcg atattttttc
    33601 agttcttcga gatagcgtcc ggcgcggaac aggaacatgc cgctgttcca gtaatattcg
    33661 ccgcttgcta cataggcctg ggcggtttcc agattcggtt tttcgacaaa ctgcgccact
    33721 tcaaaggcca ccgtatcctg ctcacccgca gacacttcac cgcgacgaat atagccataa
    33781 ccggtttctg gtagatccgg cacaatgcca aaggtcacca gcttgcccgc ttcggcataa
    33841 ggcatggcat tacgcacggc ggcacggaac gcgtctttat cggcaatcac atgatccgcc
    33901 gccaatacca gcattaacgg gtcgctctcc gggctatgac gttttgccgc cagcgccgcc
    33961 agtgcaatgg caggtgcagt gttacgccct gccggttcga gaataatgtt ctcggtgagt
    34021 ttgttcagtt gacgcagctg ttccgcgaca ataaagcggt gctgctcatt gcaaatcacc
    34081 accgggcttt cgcactccac gccgttcagg cggcagatgg tggtttgcag catggtgaga
    34141 tcgcctttca ggcataaaaa ctgcttgggg taaagtacgc gggaaagcgg ccataaacgg
    34201 ctaccggagc cacctgccat cacaactgga tagagtttcg actgcgccat aattatcccc
    34261 gaatatcatt tataaattga cgtaacacgt tctctttatc gagcgtgcgt tcggcatatt
    34321 cacgtgccac cgtgttatgt ttgggcagca ggagcgcctg acgaatcccc gccaccagcg
    34381 cctcgaccga ttccggttct acgcaaaccg caatacccgg aaaggtttcg caaagctgcc
    34441 ccagttctgt gtgggcctct gcggtaatca ccgcgttacc gcctaccgcc agaatattgg
    34501 tcagtttcga cggcaatacg gcatctgccg cgccgcgttt ttacaccacc agatggcaat
    34561 cgcccatctt cagcagtgcg ggtaaagcgt catacgattg cagcgggaaa aattgcatgt
    34621 tgcgcagtcc acgctgctgc gccatttttt ccagccgcgc tttgccgccg ccctgcccga
    34681 caatggcaaa aagcagcggt tcatcgcgca ggagatcggc agcttcaata acgttttcca
    34741 gcccctgctt ttcaccaata ttgccggagt aaagaatgat ttttttgtta tccggcaggc
    34801 caagctggtt acgaagggca tcaacatcgg cgtctgcaac atgctgaaaa cgggcaattt
    34861 ccgaccagtt ggggaagaag atgacgttat ccgccgccac gcctttttcg atggctttat
    34921 tcatcatcga acgcgaaatc gtggaaacgt tatcgacgtt atgcagtccg ctacgttcga
    34981 acgccgttgc cagctgtgcc actttgccgc ctttgccttt tccggcaagg cccagcccca
    35041 gcatggcgtc cacttcgtaa tcctgaatat gcagcacggt acgcgcacca gagagcttcg
    35101 ccagcaggcg cattcccggc gtgcaaaaca gcgttggcac tacgccaata atgcgatccg
    35161 gcttccagcg acgttgcgcc atcagtggaa agaaactgct gacggcaaaa ctgccgagat
    35221 gcaacaagcg tttcagggtg ctcggctgtt ttggcacgta cagcgggcag cgccacaccg
    35281 tggcggcccc ttcttctcgt ttgtagcgcc aggcggaata gttctcaccc acctgccatt
    35341 gcgggtagta aggcggtgcg gtaatgaccc gcacctcatg accttgtgcc gccaaccatt
    35401 ccaccatctc gccggtgtat ttgccgatgc cggttaactc cggcgagtag ttaatgccgt
    35461 agaccagtat tttcataatc cgggtactcc ggcacgcttc tcagcgagaa aataggcgcg
    35521 gctgttagcg tgaacatcgt tactggcgag cagcgcgtcc ggcgtcagcc agcggtaatc
    35581 gtcatgctgc tcatccggca gtaacagctc ttcttccgct actctgaagc gaaaaccgag
    35641 caccacatag tgagtggtga aatccgtgcc ggagaagtta tcgtcataaa aatgctgcca
    35701 gacaccgtaa aactggcctg ctgttatcgg cagacgcagc cccagttccg ccatcgtcag
    35761 ccgctcaaat gcggcttcca gcgtttcgtc tttctgcacg cgccctcccg gcacaaacca
    35821 gtaaccctgc gccgggcggt tggttctttt gccaagcaga aactcgccgc gactgttctc
    35881 gacaataaag tcgagagaga caagcggtgt ggagcgcact acagtggcaa agtcttcctg
    35941 acgtaaaaac atcattcccc ccgaaagcgg tcttgattct caaggaacca ctggtaagtg
    36001 ctggcaagcc ccgcttccag tgagatttcg tgataccagc caagctgatg caggcgcgtc
    36061 acatccagca gtttgcgcgg cgtaccatcc ggtttgctgg catcaaaaac cacccgacct
    36121 ttgtaaccca ccactttggc gatggtttgc gccagctcgc ggatagtgca gtcaacgccc
    36181 gtgccgacgt taatgtgcga cagcatcggt tggctattct ccagccagac ttcatgcgcc
    36241 agctccatga catgaatgct cgccgccgcc atatcatcga cgtgcaggaa ttcacgcatc
    36301 ggtgtaccgc tgccccatac caccacgtcc ggcgcattct gtgccgtcgc ctcgtggaag
    36361 cgacgcagca atgctggtat cacatgggag ttactcgggt ggaagttgtc atgcggcccg
    36421 tacaggttgg tcggcatgac agagcggtaa tcgcgtccat actggcggtt gtaagattcg
    36481 cacagtttga tcccggcgat tttggcaatg gcgtaaggct cgttggtcgg ctccagcgtg
    36541 ccctgcaata actcgctttc tgccatcggc tgttttgcca gtttcgggta gatgcaggac
    36601 gatccgagaa acatcagttt gttcacgtcg ttctgatgcg cggcgtgaat gatgttgctc
    36661 tcaatcatca tgttctggta gatgaaatcc gccggatagg tgttgttggc aacaatgccg
    36721 cccactttcg ccgccgccag atagacctgg tcaatgcgtt cgctggcaaa gaaatcatgc
    36781 acggcgcggc tgtccagcag gttcagttca tcgcgggtac gtaataccag ctccacatca
    36841 ccgcgctgtt cgagctgccg cttgatggca gaaccgacca tcccacaatg gccagcgata
    36901 aaaatgcgtt gtttacttat gcttatgact ccagcgcgat cgccacgtcg tagccgtgag
    36961 atttcagcag agagtgtttt ttcgccgctt cgaggtcatt agccaccatt tcagacacca
    37021 tctctctgag ggtgatttcc ggtttccagc ccagtttttc gtgcgctttg gtcgggtcgc
    37081 cgagcagcgt ttcaacttct gccggacgga agtaacgcgg atcaacggcg ataatcacat
    37141 cacccggttt aacgcccggc gcgtcatgcc cggtgacgga aaccacaatg cccttctctt
    37201 caacacccgt gccttcaaag cgcagtttga tgcccagctg tgctgccgcc atttccacaa
    37261 actgacgtac ggagtactga acgccggtcg cgataacgaa atcttccggc tgttcctgtt
    37321 gcagcatcat ccactgcatt tttacgtagt ctttggcgtg gccccagtca cgcagggaat
    37381 ccatatttcc gaggtacagg cacgactcca gcccctgggc gatgttggcg attgcgcggg
    37441 tgattttgcg ggtaacgaag gtttcgccgc ggcgcgggga ttcatggttg aagagaattc
    37501 cgttacaagc gtacatgccg taggattcgc ggtagttaac ggtgatccag taggcgtaca
    37561 gtttggcgac cgcatacgga gatcgcgggt agaacggcgt ggtttctttc tgcggaattt
    37621 cctgcaccag accatacagt tcagaggtgg aagcctgata gaaacgagtt ttcttttcca
    37681 gaccgaggaa gcggatcgcc tccagcaggc gcagcgtacc catcgcgtcg acgtcagcgg
    37741 tatattctgg tgactcaaaa gagaccgcaa cgtggctcat tgcgcccagg ttgtacactt
    37801 catccggctg tacttcacgc aaaatgcgcg tcaggttaga ggtatcactc aggtcgccat
    37861 aatgcagatg gaatttcggg ttgcaggtgt gcggatcctg ataaatgtga tccacgcgct
    37921 cggtgttgaa tgacgatgcg cgacgcttaa taccatgcac ctcgtaacct ttttccagca
    37981 gaaactctgc caggtaagaa ccgtcttgtc cggttacacc ggtgatgaga gcgacttttg
    38041 acatgtctta ttcctctgta tttttgaatt tattcagttt caacgcgttc gcgtatcacc
    38101 actgcgggat tcccccggca aaccacattt gccggaagcg atttaaaaac actgcttcgt
    38161 gcacccacga cggtgccgtc gccgattgtg acgcctgggg caacaaagac atcggttgcc
    38221 agccagcatt tctcgccaat cacaataggc gtggcgttaa tggtgaaatg ttgacttgca
    38281 tggtcgtggc taccggtgca taaataactt ttttgcgata tcaccgaatg tgcgccaatg
    38341 gttatttcac cgagggtata taaattgacg tcatcgccaa cccacgcgta atcacctaag
    38401 gttaatttcc acggataggt aatttttact gacggacgaa taactacgtt ttttcctatt
    38461 tttgctccga ataaacgtaa taaaaaagcc cgccagcgat acaatacttg tggcgaccag
    38521 gcaaatattg ttgcctgtac tgcccaccat aattgcactt taatagcgtt gccgccccgg
    38581 aaccctttcg gcaccgagaa accgcttaaa tcttgcattg gttttcctta tattcagact
    38641 ttgttatata aggctttagt cttacctgtc gtacgttggc gtaaatgcca ggataattct
    38701 gcccagaagc caggtacatg taatatctgc cgttggactt ttttggcgtc agcac
//
LOCUS       DAETPZ010000029        37869 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.029, whole genome shotgun sequence.
ACCESSION   DAETPZ010000029 DAETPZ010000000
VERSION     DAETPZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37869
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.029"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..2005
                     /gene="speF"
                     /locus_tag="K0W59_001726"
                     /pseudo
     CDS             1..2005
                     /gene="speF"
                     /locus_tag="K0W59_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            2002..3321
                     /gene="potE"
                     /locus_tag="K0W59_001727"
     CDS             2002..3321
                     /gene="potE"
                     /locus_tag="K0W59_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HBI3022801.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGTSLSPVQIGLATIGVQWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLPLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(3377..5017)
                     /gene="pgm"
                     /locus_tag="K0W59_001728"
     CDS             complement(3377..5017)
                     /gene="pgm"
                     /locus_tag="K0W59_001728"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HBI3022802.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5043..5588)
                     /gene="seqA"
                     /locus_tag="K0W59_001729"
     CDS             complement(5043..5588)
                     /gene="seqA"
                     /locus_tag="K0W59_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HBI3022803.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5773..6537
                     /gene="ybfF"
                     /locus_tag="K0W59_001730"
     CDS             5773..6537
                     /gene="ybfF"
                     /locus_tag="K0W59_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBI3022804.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            6677..6970
                     /gene="ybfE"
                     /locus_tag="K0W59_001731"
     CDS             6677..6970
                     /gene="ybfE"
                     /locus_tag="K0W59_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HBI3022805.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7110..7640
                     /gene="fldA"
                     /locus_tag="K0W59_001732"
     CDS             7110..7640
                     /gene="fldA"
                     /locus_tag="K0W59_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HBI3022806.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7850..7936
                     /gene="uof"
                     /locus_tag="K0W59_001733"
     CDS             7850..7936
                     /gene="uof"
                     /locus_tag="K0W59_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HBI3022807.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7929..8375
                     /gene="fur"
                     /locus_tag="K0W59_001734"
     CDS             7929..8375
                     /gene="fur"
                     /locus_tag="K0W59_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HBI3022808.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8459..8785)
                     /locus_tag="K0W59_001735"
     CDS             complement(8459..8785)
                     /locus_tag="K0W59_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022809.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8822..10228)
                     /gene="chiP"
                     /locus_tag="K0W59_001736"
     CDS             complement(8822..10228)
                     /gene="chiP"
                     /locus_tag="K0W59_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HBI3022810.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSYFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYNDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(10806..12470)
                     /gene="glnS"
                     /locus_tag="K0W59_001737"
     CDS             complement(10806..12470)
                     /gene="glnS"
                     /locus_tag="K0W59_001737"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HBI3022811.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(12673..14619)
                     /gene="nagE"
                     /locus_tag="K0W59_001738"
     CDS             complement(12673..14619)
                     /gene="nagE"
                     /locus_tag="K0W59_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HBI3022812.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVIINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            14952..15752
                     /gene="nagB"
                     /locus_tag="K0W59_001739"
     CDS             14952..15752
                     /gene="nagB"
                     /locus_tag="K0W59_001739"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBI3022813.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            15812..16960
                     /gene="nagA"
                     /locus_tag="K0W59_001740"
     CDS             15812..16960
                     /gene="nagA"
                     /locus_tag="K0W59_001740"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBI3022814.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAGAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPNFKITKTIVNGNEVVTQ"
     gene            16969..18189
                     /gene="nagC"
                     /locus_tag="K0W59_001741"
                     /gene_synonym="nagR"
     CDS             16969..18189
                     /gene="nagC"
                     /locus_tag="K0W59_001741"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HBI3022815.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALKERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18237..18979
                     /gene="nagD"
                     /locus_tag="K0W59_001742"
                     /pseudo
     CDS             18237..18979
                     /gene="nagD"
                     /locus_tag="K0W59_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            19376..21040
                     /gene="asnB"
                     /locus_tag="K0W59_001743"
     CDS             19376..21040
                     /gene="asnB"
                     /locus_tag="K0W59_001743"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HBI3022816.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYARDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            21420..21496
                     /locus_tag="K0W59_001744"
     tRNA            21420..21496
                     /locus_tag="K0W59_001744"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21454..21456,aa:Met,seq:cat)
     gene            21505..21589
                     /locus_tag="K0W59_001745"
     tRNA            21505..21589
                     /locus_tag="K0W59_001745"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21539..21541,aa:Leu,seq:tag)
     gene            21613..21687
                     /locus_tag="K0W59_001746"
     tRNA            21613..21687
                     /locus_tag="K0W59_001746"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21645..21647,aa:Gln,seq:ttg)
     gene            21722..21796
                     /locus_tag="K0W59_001747"
     tRNA            21722..21796
                     /locus_tag="K0W59_001747"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21754..21756,aa:Gln,seq:ttg)
     gene            21812..21888
                     /locus_tag="K0W59_001748"
     tRNA            21812..21888
                     /locus_tag="K0W59_001748"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21846..21848,aa:Met,seq:cat)
     gene            21937..22011
                     /locus_tag="K0W59_001749"
     tRNA            21937..22011
                     /locus_tag="K0W59_001749"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21969..21971,aa:Gln,seq:ctg)
     gene            complement(22165..23340)
                     /gene="ubiF"
                     /locus_tag="K0W59_001750"
     CDS             complement(22165..23340)
                     /gene="ubiF"
                     /locus_tag="K0W59_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HBI3022817.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGVGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            23486..24910
                     /gene="miaB"
                     /locus_tag="K0W59_001751"
     CDS             23486..24910
                     /gene="miaB"
                     /locus_tag="K0W59_001751"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBI3022818.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            25063..26103
                     /locus_tag="K0W59_001752"
     CDS             25063..26103
                     /locus_tag="K0W59_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBI3022819.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAREEAEQKRKAAL
                     AAERKREEQEQK"
     gene            26100..26567
                     /gene="ybeY"
                     /locus_tag="K0W59_001753"
     CDS             26100..26567
                     /gene="ybeY"
                     /locus_tag="K0W59_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBI3022820.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     MVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            26657..27535
                     /gene="corC"
                     /locus_tag="K0W59_001754"
     CDS             26657..27535
                     /gene="corC"
                     /locus_tag="K0W59_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HBI3022821.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFGMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            27560..29098
                     /gene="lnt"
                     /locus_tag="K0W59_001755"
     CDS             27560..29098
                     /gene="lnt"
                     /locus_tag="K0W59_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HBI3022822.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            29495..30402
                     /gene="gltI"
                     /locus_tag="K0W59_001756"
                     /pseudo
     CDS             29495..30402
                     /gene="gltI"
                     /locus_tag="K0W59_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
     gene            30572..31312
                     /gene="gltJ"
                     /locus_tag="K0W59_001757"
     CDS             30572..31312
                     /gene="gltJ"
                     /locus_tag="K0W59_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HBI3022823.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTASRVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLT
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            31312..31986
                     /gene="gltK"
                     /locus_tag="K0W59_001758"
     CDS             31312..31986
                     /gene="gltK"
                     /locus_tag="K0W59_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HBI3022824.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            31986..32711
                     /gene="gltL"
                     /locus_tag="K0W59_001759"
     CDS             31986..32711
                     /gene="gltL"
                     /locus_tag="K0W59_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HBI3022825.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            32829..33764
                     /gene="rihA"
                     /locus_tag="K0W59_001760"
     CDS             32829..33764
                     /gene="rihA"
                     /locus_tag="K0W59_001760"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HBI3022826.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTLGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            33848..35518
                     /gene="hscC"
                     /locus_tag="K0W59_001761"
     CDS             33848..35518
                     /gene="hscC"
                     /locus_tag="K0W59_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HBI3022827.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDKQRKHTRLAADLAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASASDNFLGGEDFTHMLVDDVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(35578..37029)
                     /gene="djlC"
                     /locus_tag="K0W59_001762"
     CDS             complement(35578..37029)
                     /gene="djlC"
                     /locus_tag="K0W59_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HBI3022828.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIINRMLLAKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(37026..37733)
                     /locus_tag="K0W59_001763"
     CDS             complement(37026..37733)
                     /locus_tag="K0W59_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBI3022829.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         9257 a         9688 c         9587 g         9337 t
ORIGIN      
        1 aaatgaagaa cgtgtaccag cagaatatct gccacgtatt tctggtgtct tcgagcattg
       61 cgaatcacgg aaagagtttt atggacgtca gttagaaacc gctgctagcc attatgaaac
      121 gcaactgcgc ccgcccttct tccgcgcact ggtcgattat gtcaatcagg gcaatagcgc
      181 ttttgactgc ccgggtcatt agggaggtga gtttttccgt cgtcatccgg cagggaatca
      241 gttcgtggag tacttcggag aaacgctgtt ccgttccgac ttatgcaacg cagacgtagc
      301 aatgggcgat ctgctgattc acgaaggcgc gccatgcatt gcacagcaac atgcggcaaa
      361 agtgtttaat gccgataaaa catacttcgt tctaaatggc acttcatctt ctaacaaagt
      421 ggttttaaac gccctgctaa caccgggtga tctggtgctg tttgatcgca ataaccacaa
      481 atctaaccac cacggagcgt tgctacaggc tggtgcaaca ccggtttatc tggaaacggc
      541 acgtaacccg tatggcttta tcggtggcat tgatgcgcac tgttttgaag aaagttacct
      601 gcgtgagctg atcgcggaag tcgcaccgca gcgggcaaaa gaggctcgtc ctttccgcct
      661 cgctgtgatt cagttaggca cctacgacgg tacgatttat aacgcccgcc aggtggtgga
      721 taaaattggt catctgtgtg actacattct gtttgactcc gcatgggttg gctatgagca
      781 atttatcccg atgatggctg actgttcgcc gctgttgctg gatcttaatg agaacgatcc
      841 gggtattcta gttacgcaat ccgtacataa acagcaggct ggtttttctc aaacttcaca
      901 aatccataaa aaagacagcc acatcaaagg gcaacagcgt tatgtaccgc acaaacgcat
      961 gaacaacgcc tttatgatgc acgcctccac cagcccgttc tatccgctgt ttgccgcact
     1021 ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt aatatgtgga tggattgtgt
     1081 ggtaaatggc attaatgccc gcaaactgat cctcgataac tgtcagcata ttcgtccgtt
     1141 cgtacctgaa ctggtggatg gtaaaccctg gcagtcgtat gaaacagcgc aaattgcggt
     1201 tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg cattcttttg aaggctatgc
     1261 agagaatcaa tactttgtcg atccttacaa actgttgctg acaaccccag gtattgatgc
     1321 acgtaacggc gaatatgaag cgttcggtgt acccgcgacg attcttgcta acttcctgcg
     1381 cgaaaatggc gtagtgccgg aaaaatgcga tcttaactcc atcctcttcc tgctgactcc
     1441 ggcagaagat atggccaaac ttcagcaact tgttgccctg ctggtacgct tcgaaaaact
     1501 gcttgagtcc gacgcgccat tagcagaagt gctgccttcc atctacaaac agcatgaaga
     1561 gcgctacgcc ggttataccc tgcgtcagtt gtgccaggaa atgcatgatt tgtatgcccg
     1621 ccacaacgtg aaacaactgc aaaaagagat gttccgcaaa gcgcacttcc cacacgtcag
     1681 catgaatccg caagaagcca actacgccta tttacgcggt gaagtggagc tggttcgtct
     1741 gccggatgcc gaaggtcgta tcgctgccga aggggcgctt ccttatcctc cgggtgtgct
     1801 gtgtgttgtt ccgggtgaaa tctggggtgg tgctgttttg cgttacttca gcgctctgga
     1861 agaagggatc aacctgctgc caggttttgc accggagctg cagggtgtct atatcgaaga
     1921 acatgatggt cgtaagcaag tttggtgcta tgtcatcaag cctcgtgatg cgcaaagcac
     1981 cctgttgaaa ggggaaaaat tatgagtcag gctaaatcga acaaaatggg cgtcgttcag
     2041 ttaaccatac tgacgatggt caacatgatg ggctccggta tcatcatgct gccgacaaag
     2101 cttgccgaag ttgggacaat ttcaattatc tcctggctgg tgacagccgt aggctcaatg
     2161 gcactggcat gggcattcgc aaaatgcggt atgttcagcc gtaaatcagg tggtatgggc
     2221 ggttatgccg agtatgcttt cggtaaatcc ggtaacttta tggcgaacta tacctacggc
     2281 gtctcactgc tgattgctaa cgtcgcgatt gctatttcgg cggttggtta cggcaccgaa
     2341 ctgctcggca cgagtttgtc gccagtgcag attggtcttg cgaccatcgg ggtgcagtgg
     2401 atttgtaccg tggctaactt tggtggtgcg cgcattaccg ggcaaatcag tagcattacc
     2461 gtgtgggggg tcattattcc ggtggtaggc ctgtgcatta tcggctggtt ctggtttagc
     2521 ccgacgcttt acgttgattc ctggaatccg catcatgcac cgttcttcag tgcagtaggt
     2581 tcttccatcg ctatgacgct gtgggctttt cttggtctgg agtctgcgtg tgcgaatact
     2641 gatgtagtgg aaaacccgga acgtaatgtg ccaatcgcgg tactcggcgg tacgttaggt
     2701 gcggcggtga tttatatcgt ctccaccaac gtgattgcag ggattgtgcc taatatggag
     2761 ctggcaaatt caacggcacc atttggcttg gccttcgcac agatgttcac accagaagtg
     2821 ggtaaagtca ttatggcgct gatggtgatg tcctgctgcg gttcgctgct tggctggcag
     2881 ttcaccattg cccaggtgtt taaatcttca tctgatgaag gctacttccc taaaattttc
     2941 tcccgtgtaa ccaaagtgga tgcaccggtg cagggaatgt tgaccattgt gattatccag
     3001 agcgggttgc cgctgatgac gattagcccg tcgctaaaca gtcagttcaa cgtgctggtt
     3061 aacctggccg tggtgaccaa tatcattccg tatattctgt caatggcggc attagtcatt
     3121 attcagaagg tcgctaatgt gcccccctca aaagcgaaag ttgcaaactt tgttgctttt
     3181 gttggcgcga tgtatagctt ttatgcgctg tactcatccg gggaagaagc catgctgtac
     3241 ggttccatcg tgaccttcct tggttggaca ctgtatggtc tggtatcacc acgctttgaa
     3301 ctgaaaaata aacacggtta aaaaagcaaa gtaaaaaagg gcgatcttgc gaccgccctt
     3361 tttttattaa atgtgtttac gcgtttttca gaacttcgct aacaatctca accgcttctt
     3421 tctcaatctg cttgcgatgt tcttcaccga ggaagctttc gcagtagatc ttatatgcgt
     3481 cttccgtgcc tgacggacgc gcggcgaacc agccgttgtc agtcatcact ttcagaccgc
     3541 caatagaagc accgttgccc ggagcagcag tcaggcgcgc ggtgatcggg tcacctgcca
     3601 gggtgctggc gctcaccatt tccggagaca gcttagacag cgccgctttt tgtgcggaag
     3661 tcgcagctgc ctgcaaacgg ttgtagctcg gcgcaccaaa gcgttttgcc agttcgttgt
     3721 agtgttcctg cgggttctta ccggtgacag cggtgatttc cgccgccagc agacacataa
     3781 tgatgccgtc cttgtcggtg gaccacggcg tgccgtcgaa acgcaggaag gaagcccctg
     3841 cgctctcttc gccgccaaag ccgaagctgc cgtcgaacag accatcaaca aaccatttga
     3901 aacctaccgg tacttctacc agcttacggc ccaagtcatt gaccacacgg tcgatcatcg
     3961 cagaagaaac cagcgtttta ccgacagcaa catctttgcc ccactgcgga cgatgctgga
     4021 acaggtagtt gatcgccacc gccaggtagt ggttcggatt catcaaacct gccggagtga
     4081 cgataccgtg acggtcataa tccgggtcgt tagcaaacgc cagatcgaac ttatcacgca
     4141 gtgccagcag acccgccatc gcacactcgg aggagcagtc catacggatc gcaccgtctt
     4201 tatcgaggtg cataaagcgg aaggtttgat cgacctgatc gttaacgatg gtcaggttga
     4261 ggttgtaata ctcgccaatg cgcttccagt attcgatacc ggaaccgccc aacggatcaa
     4321 cgcccagcgt caggcccgct ttctgaatcg ccgccatatc aacgatatcg gccagccctt
     4381 ccacgaacgg ctgcaccaga tcctgctctt tcacatagcc ggatgccatc gcttcgtcga
     4441 gggagatacg cttcacgcct ttcaggccat cggccatcag tgcgttggcc ctgtcttcca
     4501 ccactttggt gacgttagta tcagccgggc caccatttgg cggattgtac ttgataccac
     4561 catcttccgg cgggttatgg gacggtgtaa tcacgatacc gtctgccagc gggccacctt
     4621 ttttattgtg aaccaggatg gcattggaaa cggcaggcgt cggagtgaag ccattgtttt
     4681 cttgcacaat gacatcaacg ccgttcgctg ccagcacttc cagtacggaa atgaacgcag
     4741 gctcggacag ggcgtgagta tctttaccca catagcaagg gccagtgatg ccgtttttcg
     4801 cacgttcttc agcaattgcc tgagcgatcg ccagaatgtg cggctcgtta aagctgtggc
     4861 gcgctgcact gccacggtga ccggaagtac cgaatttcac cgcgtgctcc gcattccctg
     4921 cttctggttt cagtacataa tattgcgccg tcagttgggc gacgttaatc aaatcactct
     4981 gttgtgcagg ttggcctgca cgattgtgga ttgccattgc tttgtccttt gtctgcaacg
     5041 ttttagatag ttccgcaaac cttctcaatc aattccgccg ggaattgcat cgactgcatg
     5101 atgtgttcga tcatgctgca tttacggccg gtgttggtgt tggtgatcac ccaatacggc
     5161 gtgcctggca catgtttcgg cttggtctga ttaccatttt tcagcagcgt ttgttcatct
     5221 gccgcaaagt acacgcgtgt acgaccgtgc aacgattccg ttgcttcggc aaacgcctgg
     5281 gcgtcaagag aatatagtgt agacaacagc agcataaagc gattgaccgc tcgcttttgc
     5341 tctgcgtatt cgtccgaaag cagaagttca cgcattgcgc gaaccttgtc tttaatcgtt
     5401 ttgaccggct tcgcttcgac gatagcaggt gacgcaacgc gaacctcttt cgtcaccgga
     5461 gcagcaggct gtgatgcggc ggaaaatttc aacatacgcc gtaaaatgtc ggatgcgctc
     5521 tcgccgatat gcttagtgtg gctggcaata tagctgtaga gttcatcatc aacttcaatc
     5581 gttttcatct taatccagtg cagtgtctta gctgaataca agtcgccaag agtatatgaa
     5641 taaatcccgg cagcggatag cgtcaagcct ggcttgcaga aaaactcgga ataaacctta
     5701 tttgttgcag ccctgtggca caatggtcaa cataataccc taacccgaca acgcgaaaaa
     5761 aagaactttg ccatgaaatt aaatatccgc gcgcaaactg cacaaaacca gcacaataat
     5821 tctcccatcg ttcttgtcca tggtctgttt ggtagcctcg acaaccttgg cgtactggct
     5881 cgcgatctgg taaacgatca caatatcatc caggttgata tgcgtaacca cggtctttca
     5941 ccaagagagc cggtaatgaa ttacccggcg atggcgcagg atcttgttga taccctggat
     6001 gcactgcaga tcgacaaagc aacatttatc ggtcactcca tgggcggtaa agcggtaatg
     6061 gcacttactg cactggcccc cgatcgcatc gataaactgg tggcgatcga tatcgcaccg
     6121 gtcgactatc acgtacgccg tcatgatgag attttcgcag ctatcaacgc ggtcagtgaa
     6181 tcggacgcac aaactcgcca gcaagcagca gcaataatgc gccagcatct taatgaagaa
     6241 ggggtgattc agtttctgtt gaaatctttt gttgacgggg agtggcgctt taacgtaccg
     6301 gtattgtggg atcagtatcc gcatattgtg ggttgggaga aaatcccggc atgggatcac
     6361 cctgccctgt ttatccctgg cggcaattct ccgtatgtta gcgagcagta ccgtgatgat
     6421 ttactggcac aatttccaca ggcacgggcg catgtgattg caggcgcggg tcactgggtc
     6481 catgctgaaa aaccggatgc ggtattacgc gctatccgtc gctatctcaa tgattaacgc
     6541 aactgattaa aaacccagcg tccgcgtcgc tttccgcgcg tgggcgttgg cgtgactcat
     6601 cgcgctgatg tattatggcg cgctatccat ccgggcagag gcctggctga ttgtttcccc
     6661 cgaagtcacc aagatcatgg ccaaagaaca aacggaccgt acgacattag atctgttcgc
     6721 gcacgagcgt cgaccgggac gaccgaaaac taatccgctt tcgcgcgatg aacagctgcg
     6781 tattaataaa cgcaaccagc taaaacgcga caaagtacgt ggccttaagc gtgtcgaact
     6841 gaagctgaac gcggaagctg tcgaggcgct gaacgagctg gcggaatcgc gtaatatgag
     6901 ccgtagcgaa ctgatcgaag agatgttaat gcagcaactg gcggcgctgc gtagtcaggg
     6961 catcgtttaa atttccactt tcatgtagca cagtgtgcag tcctgctcgt ttgctgattc
     7021 cgcaagactg cctgttctgc tatgattgcc tttatccgtg ggcaattttc cacccccatt
     7081 tcaataagtt tcaagaggtt atttcactca tggctatcac tggcatcttt ttcggcagcg
     7141 acaccggtaa taccgaaaat atcgcaaaaa tgattcaaaa acagcttggt aaagacgttg
     7201 ccgatgtcca tgacattgca aaaagcagca aagaagatct ggaagcttac gacattctgc
     7261 tgctgggcat cccaacctgg tattacggcg aagcgcagtg tgactgggat gacttcttcc
     7321 cgactctcga agagattgat ttcaatggta aactggttgc gctgtttggt tgtggtgacc
     7381 aggaagatta cgccgaatat ttctgcgacg cattgggcac catccgcgac atcattgaac
     7441 cgcgcggtgc aaccatcgtt ggtcactggc caactgcggg ctatcatttc gaagcatcaa
     7501 aaggtctggc agatgacgac cactttgtcg gtctggctat cgacgaagac cgtcagccgg
     7561 aactgaccgc tgaacgtgta gaaaaatggg ttaaacagat ttctgaagag ttgcatctcg
     7621 acgaaattct taatgcctga tgtgatgcgg cgtagactca tgtctacgcc gtattaatag
     7681 ataatgccaa tcaaaataat tgctacaaat ttgtaacttt tgctgttgta cctgtacaat
     7741 gtcccagtgt tcaagtggcc ttgccgttgt aaatgtaagc tgtgccacgt ttttattaac
     7801 aatatttgcc agggacttgt ggttttcatt taggcgtggc aattctataa tgatacgcat
     7861 tatctcaaga gcaaattctg tcacttcttc taatgaagtg aaccgcttag taacaggaca
     7921 gattccgcat gactgataac aataccgccc taaagaaagc tggcctgaaa gtaacgcttc
     7981 ctcgtttaaa aatcctggaa gttcttcagg agccggacaa ccatcacgtc agtgcggaag
     8041 atttatacaa acgtctgatc gatatgggtg aagaaattgg tctggctacg gtatatcgcg
     8101 tactgaacca gtttgacgac gctggtatcg tcacccgcca caattttgaa ggcggtaaat
     8161 ccgtatttga actgacacag caacatcacc acgatcacct gatttgcctc gactgcggca
     8221 aggttatcga atttagtgat gattccatcg aagcgcgtca gcgtgaaatt gccgcaaaac
     8281 atggcattcg cctgactaac cacagtctct atctttacgg tcactgtgcc gaaggcgatt
     8341 gccgcgaaga tgagcacgcg cacgaaggca aataagccag cctgaacgag aaaagccaac
     8401 ctgcgggttg gcttttttat acaagggaaa taagaaaagc gagagttaca gctctcactt
     8461 atttgttatt actgcgtatt tcctgccaga ccttatcaca atcggctttc accgcctgat
     8521 catttccggt ttgcgttgca cgcagacact gctgataatc cagtacgcgt acactttcct
     8581 ggtcggcaaa ctgctgatgt tgtttctctt tcttcagcac gtttaacacg ctctgacagg
     8641 cttcaatttt ttctggcgaa ccttgcgcgg tgttgataca ggcgctgtat gcctctttca
     8701 gacggctgtc ttcctgtggt gcggttgatt gcgcacaagc taccagcccc gatgccatca
     8761 tggcgatgag aatcagtttt ttcattgaat gcggcaggca caaaacctgc cgcgtcgggc
     8821 atcagaagat ggtgaatggt gcgattacca taaatttcac gtcacgctca tcctggaaga
     8881 tgttaccgta accgccgccc cagcttggga tgtcggagtg gttgtcgtat tcggtgaagt
     8941 gcagtttgaa catcgtgcct ttggcgcgac cgtcctgaat ggtgtagacc gcatccaggc
     9001 tgtaggcaga ctcttcaata gtccggtttt tgtcgttgta cgcatccgga ttgctctgcc
     9061 aggtcgcagg tttagcatcc catgcgtaaa cgtaggaagc gccgatggcg aagcctggaa
     9121 gattccagtt tttcaggtca tacatcgcac cgaagaagac cgctttttcg ccgttagcgt
     9181 tgaagtagga acggttatcc caccagatat ccaggcggcc gttggaggaa gcgtaggttg
     9241 gggtcatacg ttgcaggaag tatccctgct gactgtcagc cttaacccag gtgccttcga
     9301 ggcgcaaatc cactacgtca gccgcccggt aaccaaaggt caacgcttgc agccaagcgg
     9361 tgccgtcata aagatcgttg acgctgcgat cgtcaacttt atcgcgggta ccatagaact
     9421 ggtagctggt ggttaacggg ctaccggcga tatcaaattt gtagctggct ttggcaaaat
     9481 attgatcgat atacccttcc gcctgaccaa atgccgcttc cagtacgaag ttatttttga
     9541 agtcgtattt cgcaccgatg gagtgcagat aatcaacttt ggtggtttta tcgttctgat
     9601 aaaactcatc catttccaga tgccacggcg ctttgtattc gttggtccac atgtaggaga
     9661 aactcaaggc accagcatcg ccgtaatcaa aattcgcccc ggcttccgcc ccctgataag
     9721 tacccggcat aaagctccag tgcggcgcta acagcgtttg accggttggc tgaatgtaac
     9781 ctgccctcgc ccaaaccgga ccgtatttaa atttggccgc agctttatac aggcttatac
     9841 cgcttttgtc gccggaccag tcttcgtcat aggctttatt actttttgaa aacgcgattt
     9901 cgttcgggtg ggagctgtcg ccgttttccg ccatttcaat cgccgtaaac gcagcaatat
     9961 caaggccgaa catatcagca gcataaccgg actgaaaatc gaggttggca ttccaggtgg
    10021 agtgagaaag gttggttttg tatttgtcgc cgtcggtaac atctttacgg tcgcgttcac
    10081 gctgccagta atagataccg ccggttaagg ttgaatcgtc gatgaatcct tcggcgcgag
    10141 cctccggcat tgccataaag cccgacattg ctgtaacacc ggcaatagcc agcgccagcg
    10201 tactacgttt gccactaaac gtacgcatgg gttaatcctc tttgacgtat aaattgctgc
    10261 accaaaggtg aacagcgaaa aaaataaaaa ttaaaaaagt agcgttgaga aaagtctcgc
    10321 tgactacagg aacagactat ttttcgcgac gcgaattatc aatctctttc tgtaaccaga
    10381 atgtaagaac atgagcaacc gcacatattt tgatgatttt tgttacaagg cttaagtttg
    10441 cggttgaaag gcttgcagaa gaataaaaac gtgaaattcc agcaagatga aagcggttac
    10501 attttattta attatgtttt gtgattttaa atttatttta tggataaatt gcgaagcgga
    10561 attaattcgc gaagaaaagc agaaaaaacg ccgcagagcg cggcgtttgg gattgtttgc
    10621 tatttcttgc cggatgcggc gtgaacgcct tatccggcct acaaaagcac gcaaattcaa
    10681 tatattgcag agatcatgta ggcctgataa gcgaagcgca tcaggcaatt tagcgtttga
    10741 aatttattgt ctatttattg ccggatgcga cgtaaatgcc tcacccggct tggtagaact
    10801 taaaattact cgcctacttt cgcccaggta tcacgcagcc caacggtgcg gttaaatacc
    10861 ggtttttcgg ccgtggaatg gcggctgtcg aggcagaagt aaccttcacg ctcaaactgg
    10921 aatgctttac ccgctaccgc atctttcagc gacggttcag caaagccctg tttgatcacc
    10981 agcgattccg ggttaatcac cgacaggaaa tcatccgcag cacctgggtt aggcacgctg
    11041 aacagacgat catacaaacg gatttcaacc ggcagcgcat gtgccgcgct cacccagtga
    11101 ataacacctt tgactttacg accatctgcc ggatctttgc ttaaggtgtc ggcgtcataa
    11161 gtacagaaga tggtggtgat attaccttcg gcatctttct ccacgcgttc agccttaatg
    11221 acgtaagcat tacgcagacg cacttcttta cccagcacca gacgtttgta ctgcttgtta
    11281 gcttcttcgc ggaaatcggc gcgatcgatc caaatttcac cgctaaacgg cacctgacgg
    11341 ctccccattt ccggtttgtt cggatggttc ggcatggtga ccatttcgcc ttcgccctga
    11401 tagttttcga taaccagttt caccggatcg ataactgcca ttgcgcgcgg cgcattttcg
    11461 ttgagatctt cacggatgca ggattccagc gacgccatct caatggtgtt gtcctgcttg
    11521 gtcacgccga tgcgtttgca gaactcacga atagaagccg cagtgtaacc acgacgacgc
    11581 agaccggaaa tggtcggcat acgcgggtca tcccagcctt caacgtgctt gtcggtcacc
    11641 agcaggttca gcttacgctt ggacatcaca gtgtattcca gattcaggcg cgagaattcg
    11701 tactggcgcg gatgaacagg aatggtgatg ttatccagta cccagtcgta cagacgacgg
    11761 ttgtcctgga actcaagcgt acacagagag tgcgtaatac cttccagcgc atcgctgatg
    11821 caatgggtga agtcgtacat cgggtagatg caccacttgt tgccagtctg gtggtgttca
    11881 gcaaatttaa tgcggtacag caccggatcg cgcattacga taaatggcga cgccatgtcg
    11941 attttcgcac gcaggcaggc tttaccttct tcaaaaccac cggcacgcat tttttcgaac
    12001 agcgccaagt tctcttcaac gctgcggtcg cggtacgggc tgtttttacc cggctgcgtc
    12061 agggtaccgc ggtattcgcg gatctgttcc ggtgtcagtt catcaacgta cgccaggcct
    12121 ttattgatca gttcgatcgc ataggcgtgg agctgatcaa aataatcgga ggagtaacgg
    12181 acgttaccag accagtgaaa acctaaccac tctacgtcgt ttttgatcga atcgacatac
    12241 tcgatatctt cttttaccgg gttagtgtcg tcgaaacgca ggttgcactg gcctttatag
    12301 tcctgggcga tcccgaagtt caggcagata gatttcgcat ggccaatatg cagatagcca
    12361 ttcggctccg gcgggaaacg ggtgtgtact gtggtgtgct taccactggc cagatcttca
    12421 tcgatgatct gacggataaa gttagtcggg cgggcttctg cctcactcat cgtggattcc
    12481 tcaaagcgta aacaacgtat aacggcgtat gatcttataa gcgggacagg ctgacaactg
    12541 ttagtttttt aaaattgata atctaacaag gattatgggg atgattgatg aaaaaaaaag
    12601 cggcagagaa gatctccacc gcttgtcatt gttggatgcg acgctgccgt gtcgcatcag
    12661 gcatacagca gattactttt tgatttcata cagcggtgtt tgacccgcca caacatggcc
    12721 ctgagcttta atgatcaagc cactgaaatc gtcgatattg ctgcaaacca ccgggctaat
    12781 catcgagcgg gcgttagcgt tcaggtaatc cagatccatt tccagaatcg gttgccctgc
    12841 gcttacctgc gcaccctctt ccaccagacg tttaaagcct ttaccttcca gcgctacggt
    12901 gtcgataccc atatggacga cgatctccgc gcctttttcg gtttccaggc agaacgcgtg
    12961 gttggtgttg aagattttca cgattgtccc tgcggctggt gatacgacga ttttatctgt
    13021 cggtttcacc gccacaccgt cacccaccgc tttgctggcg aatgcttcgt caggaacctg
    13081 atccagtgcc acgacatcac cggtaatcgg cgataccagc tccgcgatag ataccgcgtt
    13141 tggtacagcc tgcggttttg ctacaggcgc ggcagttgcc ggagttgctt cagctgacgc
    13201 agcggctacc ggaccacggg caacgacttt cttcatcgca tcgccgatgg attctgcttt
    13261 cgcgccaaca atcacctgaa tagtctgttt gttcagtttc actacaccag aagcacccag
    13321 acgtttacac atcgtatcgt tgacgcgggc agagtcagcc acggtcaggc gcagacgggt
    13381 aatacaggca tcaatcgctt tcaggttgtc agtaccgcca accgcagcaa tatagttggt
    13441 tgccagttga gtcagacctt cttcagtatt gctgttggct tcttcagtaa cgatctcgtc
    13501 ttctttatct tcacgacccg gcgttttcag gttgaacatg cggataacca aactgaacac
    13561 cacgaagtag atagcgaaga agataacgcc catcaccagc agcatccaga cgttctggct
    13621 ggcggccggc aggttataca tcaacgcgta gtcgatagca cccgcagaga aagagaagcc
    13681 ggcatggata cccagcagcg ttgccacaaa caggctgata ccggtcagca gtgcgtgcag
    13741 gaggtacagc agcggcgcaa ggaacatgaa caggaattcc agcggctcag tcacaccggt
    13801 caggaacgca gtaacagcaa cagaaagcag catcccgcca accatcggac gacgctcttt
    13861 cggtgctgcg aagtacatcg ccagcgccgc acccggcaga ccgaacatca tgatcgggaa
    13921 gaagccggac atgaacatcc ccgcggtgcc gtcaccagcg tagaaacggt tgatgtcgcc
    13981 gtggaaaacc gtacccgccg cgttggtgaa ttcaccaatc tggaaccagg cgatggtgtt
    14041 cagcacctga tgcagaccgg ttgggatcag cagacggttg atgaaaccaa agataccgga
    14101 acccagcgcg cccgcagaaa cgatccactc gccgcctgca tggatagcgt gctgtaccgg
    14161 cggccagacg taaccaaaaa tggccgccag caccagacag aagaagccgg tggcgatcgg
    14221 cacaaagcgt ttgccgccga agaagctcag gaagtccggc agtttaatat cggaccaacg
    14281 gttataggct gcgccaccaa ccagaccggt aatgataccc gccagtacac ccatgttaat
    14341 ttctgggttg atgatcacca tcgctttggt taacacaaag taacctaccg cacccgccag
    14401 cgccgccgca cctgcgctgt ctttcgacca gctggatgcc acaccgatgg cgaagattaa
    14461 tgcgaggtta tcaaaaattg caccgcccgc ctgggcaata aacgcaacgt taagtaaatc
    14521 tggctgaccg aatcgcagca acagcgccgc caccggcagc accgcgatag ggagctgtaa
    14581 cgccctaccg agtcgctgga aaaaacctaa aatattcatc ttattccccc tacgagaacc
    14641 ctatttggct cgtttaaagc cgtattttta ttttgctgca aattttactg ccgatgttct
    14701 gtaatcagat tgttagatca tctgctacag agtgtgtgaa aatttaattc gtatcgcaaa
    14761 ttaaacgcgt gtcttttgtg agttttgtca ccaaatatcg ttatcatcac tcccttttac
    14821 tggctaaacc agaaaactta ttttatcatt caaaaaatca agtcggattg acgcctgtct
    14881 gcgcaaatcc aggttacgct taaagatgtc taatccgcca acggcttaca ttttacttat
    14941 tgaggtgaat aatgagactg atccccctga ctaccgctga acaggtcggc aaatgggctg
    15001 ctcgccatat cgtcaatcgt atcaatgcgt tcaaaccaac tgccgatcgt ccgtttgtac
    15061 tgggcctgcc gactggcggc acgccgatga ccacctataa agcgttagtc gaaatgcata
    15121 aagcaggcca ggtcagcttt aagcacgttg tcaccttcaa catggacgaa tatgtcggtc
    15181 tgccgaaaga gcatccggaa agctactaca gctttatgca ccgtaatttc ttcgatcacg
    15241 ttgatattcc agcagaaaac atcaaccttc tcaacggcaa tgccccggat atcgatgccg
    15301 agtgccgcca gtatgaagaa aaaatccgtt cttacggaaa aattcatctg tttatgggcg
    15361 gtgtaggtaa cgacggtcat attgcattta acgaaccggc gtcttctctg gcttctcgta
    15421 ctcgtatcaa aaccctgact catgacactc gcgtcgcgaa ctctcgtttc tttgataacg
    15481 atgtcaatca ggtgccaaaa tatgccctga ctgtcggtgt tggtacactg ctggatgccg
    15541 aagaagtgat gattctggtg ctgggtagcc agaaagcact ggcgctgcag gccgccgttg
    15601 aaggttgcgt gaaccatatg tggaccatca gctgtctgca actgcatccg aaagcgatca
    15661 tggtgtgcga tgaaccttcc accatggagc tgaaagttaa gactttaaga tatttcaatg
    15721 aattagaagc agaaaatatc aaaggtctgt aattgttatc cctgccctgg ttccttgctc
    15781 agggcaatat tttttaaaat cgggggtcag aatgtatgca ttaacccagg gccggatctt
    15841 taccggccac gaatttcttg atgaccacgc ggttgttatc gctgatggcc tgattaaaag
    15901 cgtctgtccg gtagcggaac tgccgccaga gatcgaacaa cgttcactga acggggccat
    15961 tctctccccc ggttttatcg atgtgcagtt aaacggctgc ggcggcgtac agtttaacga
    16021 caccgctgga gcggtcagcg tggaaacgct ggaaatcatg cagaaagcca atgagaaatc
    16081 aggctgtact aactatctgc cgacgcttat caccaccagc gatgagctga tgaaacaggg
    16141 cgtgcgcgtt atgcgcgagt acctggcaaa acatccgaat caggcgttag gtctgcatct
    16201 ggaaggtccg tggctgaatc tggtaaaaaa aggcacccat aatccgaatt ttgtgcgtaa
    16261 gcctgatgcc gcgctggtcg atttcctgtg tgaaaacgcc gacgtcatta ccaaagtgac
    16321 cctggcaccg gaaatggttc ctgcggaagt catcagcaaa ctggcaaatg ccgggattgt
    16381 ggtttctgcc ggtcactcca acgcgacgtt gaaagaagca aaagccggtt tccgcgcggg
    16441 gattaccttt gccacccatc tgtacaacgc gatgccgtat attaccggtc gtgaaccggg
    16501 cctggcgggc gcgatcctcg acgaagctga catttattgc ggtattatcg ctgatggcct
    16561 gcatgttgat tacgccaaca ttcgtaacgc taaacgcctt aaaggcgaca aactgtgtct
    16621 ggttaccgac gccaccgcgc cagcaggtgc caacattgaa cagttcattt ttgcgggtaa
    16681 aacaatatac taccgtaacg gactttgtgt ggatgagaac ggtacgttaa gcggttcatc
    16741 cttaaccatg attgaaggcg tgcgtaatct ggtcgaacat tgcggtatcg cactggatga
    16801 agtgctgcgt atggcgacgc tctatccggc gcgtgcgatt gacgttgaaa aacgtctcgg
    16861 cacgctcgcc gcaggtaaag tagccaacct gaccgcattc acacctaatt ttaaaatcac
    16921 caagaccatc gttaacggta acgaggtcgt aactcaataa gagaaagtat gacaccaggc
    16981 ggacaagctc agataggtaa tgttgatctc gtaaaacagc ttaacagcgc ggcggtttat
    17041 cgcctgattg accagtacgg gccaatctcg cggattcaga ttgccgagca aagccagctt
    17101 gcccccgcca gcgtaaccaa aattacgcgt cagcttatcg aacgcgggct gatcaaagaa
    17161 gttgatcagc aggcctccac cgggggccgc cgcgctatct ccatcgtcac cgaaacccgc
    17221 aatttccacg caatcggcgt acggcttggt cgtcatgacg ccaccatcac tctgtttgat
    17281 ctcagcagca aagtgctggc agaagaacat tacccgctgc cggaacgtac ccagcagacg
    17341 ctggaacatg ccctgctgaa tgccattgct cagtttattg atagctacca gcgcaagctg
    17401 cgcgagctaa tcgccatttc ggtgatcctg ccagggcttg ttgacccgga cagcggcaaa
    17461 attcattaca tgccgcatat tcaggtagaa aactgggggc tggtagaagc actgaaagaa
    17521 cgttttaaag tgacctgttt cgttggtcac gatatccgta gtctggcgct ggcggagcac
    17581 tacttcggtg caagtcagga ttgcgaagac tccattctgg tgcgtgtcca tcgcggaacc
    17641 ggggccggga ttatctctaa cgggcgcatt tttattggcc gcaacggtaa cgtcggtgaa
    17701 attggccata ttcaggtcga accgctgggt gaacgctgcc actgcggcaa ctttggctgc
    17761 ctggaaacta tcgccgccaa cgctgccatt gaacaacggg tgttgaatct gttaaagcag
    17821 ggctaccaga gccgtgtgcc actggacgac tgcaccatca aaactatctg caaagccgcg
    17881 aacaaaggcg atagcctggc ctcggaagtg attgagtatg tcggtcgtca tctgggtaaa
    17941 accatcgcca ttgctatcaa cttatttaat ccgcaaaaaa ttgttattgc tggtgaaatc
    18001 accgaagccg ataaagtgct gctccctgct attgaaagct gcattaatac ccaagcgctg
    18061 aaggcgtttc gtactaatct gccggtggta cgttctgagc tggatcaccg ctcggcaatc
    18121 ggcgcttttg cgctggtaaa acgcgccatg ctcaacggta ttttgctcca gcatttgctg
    18181 gaaaattaat gtgcttttat agtggcgctt attgttgtca atattctggg tagtccatga
    18241 ccattaaaaa tgtaatttgc gatatcgacg gcgtgctgat gcacgataac gtcgccgtac
    18301 cgggtgcagc ggaatttttg cacgggatta tggataaagg cctgccgctg gtgttgctga
    18361 ccaactatcc ttcgcagact gggcaagatc tggcgaaccg ctttgccact gccggtgtcg
    18421 atgtgccgga tagcgtgttt tatacctctg cgatggcgac cgccgatttc ctgcgtcgcc
    18481 aggaaggcaa gaaagcgtat gtggtgggcg aaggcgcact gattcatgaa ctgtacaaag
    18541 ccggtttcac tattaccgat gtgaaccctg atttcgtgat tgttggcgaa acacgttcct
    18601 acaactggga catgatgcat aaagcagcct atttcgtcgc taacggtgca cgctttatcg
    18661 ccaccaaccc ggacacggtt tttatcccgc ttgtggcgcg ttgtgtgcag ggattgagaa
    18721 aatctccggg cgcaaaccgt tctatgttgg taagcccagc ccgtggatca tccgcgcggc
    18781 attaaacaaa atgcaggcgc attcggaaga aacagtgatt gtcggcgata acctgcgtac
    18841 cgatattctg gcgggcttcc aggccggtct ggagacgatt ctggtgcttt ctggtgtttc
    18901 atcgctcgac gatatcgaca gtatgccgtt ccgcccaagc tggatttacc cgtcagtcgc
    18961 tgaaatcgac gttatctgaa taccccccac gtctgcggac gtggttggct ctgatgacaa
    19021 acgccaaact gcctgatgcg ctacgcttat caggcctacg cagctcctgc aatatattga
    19081 attttcatgc ttttgtaggc cgtataaggc gttcacgccg catccggcat gaacaaatcg
    19141 cactttatca gcaatttggc ccccgtaaaa tataaccatt caataaaacc cgctaaaaat
    19201 tatcgattca tcaataaata catcctcaca ccagaccctt tttcaccaat cgacaatcac
    19261 cattacgttt tttatttttg cactgccaaa tagcttgcgc acagtgctgc tttgcggcat
    19321 ttttttaaac aagcaaacac aacaagcaac aaataccagg ttaacggaga aggttatgtg
    19381 ttcaattttt ggcgtattcg atatcaaaac agacgcagtt gagctgcgta agaaagcact
    19441 cgagctgtca cgcctgatgc gtcatcgtgg cccggactgg tccggtattt atgcccgcga
    19501 taacgccatt ctcgctcacg aacgtctgtc aattgttgac gttaacgcgg gggcgcaacc
    19561 tctctacaac caacaaaaaa cccacgtact ggcggtaaac ggtgaaatct acaaccacca
    19621 ggcattgcgc gctgaatatg gcgatcgtta tcagttccag acagggtctg actgtgaagt
    19681 gatcctcgcg ctgtatcagg aaaaagggcc ggaatttctc gacgacttgc agggcatgtt
    19741 tgcctttgcc ctgtacgaca gcgaaaaaga tgcctacctg attggtcgcg accatctggg
    19801 gatcatccca ctgtatatgg ggtatgacga acacggtcag ctgtatgtgg cctcagaaat
    19861 gaaatcgctg gtgccagttt gccgcacgat taaagagttc ccggcgggga gctatttgtg
    19921 gagtcaggac ggcgaaatcc gttcttatta tcatcgcgac tggttcgact ttgatgcagt
    19981 gaaagataac gtgaccgaca aaaacgagct gcgtcaggca ctggaagatt ccgttaaaag
    20041 ccatctgatg tctgatgtgc cttacggtgt gctactttct ggtggtctgg attcctcaat
    20101 tatttccgct atcaccaaga aatacgcagc ccgtcgcgtg gaagatcagg aacgctctga
    20161 agcctggtgg ccgcagttac actcctttgc tgtaggtctg ccgggttcac cggatcttaa
    20221 agcagcccag gaagtggcaa accatctggg cacggtgcat cacgaaattc acttcactgt
    20281 acaggaaggt ctggatgcca tccgcgacgt gatttaccac atcgaaactt atgatgtgac
    20341 cactattcgc gcttcaacac cgatgtattt aatgtcgcgt aagatcaagg cgatgggcat
    20401 taaaatggtg ctgtccggtg aaggttctga tgaagtgttt ggcggttatc tttacttcca
    20461 taaagcgccc aacgctaaag aactacatga agagacggtg cgtaaactgc tggccctgca
    20521 tatgtatgac tgcgcgcgcg ccaacaaagc gatgtcagcc tggggcgtgg aagcacgcgt
    20581 tccgttcctc gacaaaaaat tccttgatgt ggcgatgcgc attaacccgc aggataaaat
    20641 gtgcggtaac ggcaaaatgg aaaaacacat cctgcgtgaa tgttttgagt catacctgcc
    20701 cgcaagcgtg gcctggcggc agaaagagca gttctccgat ggcgtcggtt acagttggat
    20761 cgacaccctg aaagaagtgg cggcgcagca ggtttctgat cagcaactgg aaactgcccg
    20821 cttccgcttc ccgtacaaca cgccgacctc aaaagaagcg tatctgtacc gcgagatctt
    20881 tgaagaactg ttcccacttc cgagtgccgc tgagtgcgtg ccgggcggtc cttccgtcgc
    20941 gtgttcttca gctaaagcga tcgaatggga tgaagcgttc aaaaaaatgg acgatccgtc
    21001 tggtcgtgcg gttggcgttc accagtcggc gtataagtaa gaaatgcggt actgccccgg
    21061 agaaatccgg ggctgtttta gttattgcgc tgcttgttga ttctgatatg cagtaaggaa
    21121 atgttccggg tttgccacat cactgtttga caactcatca ctgtaggcca gtatttcata
    21181 gattaatttc ccaccactat tgttgaattc cgaatttcat cctgtacgac aaaagaatgg
    21241 ctgtagggtg gggaaaaaac acaaagtaaa caataattga cgaatatagc gccacgctgt
    21301 tcgcaaccta accaaacagt cactttcgag caattttcct tgaaaaagag gttgacgctg
    21361 caaggctcta tacgcataat gcgccccgca acgccgataa ggtatcgcga aaaaaagatg
    21421 gctacgtagc tcagttggtt agagcacatc actcataatg atggggtcac aggttcgaat
    21481 cccgtcgtag ccaccatctt ttttgcggga gtggcgaaat tggtagacgc accagattta
    21541 ggttctggcg ccgcaaggtg tgcgagttca agtctcgcct cccgcaccat tcaccagaaa
    21601 gcgttgtacg gatggggtat cgccaagcgg taaggcaccg gtttttgata ccggcattcc
    21661 ctggttcgaa tccaggtacc ccagccatat tcttcgagta agcggttcac cgcccggtta
    21721 ttggggtatc gccaagcggt aaggcaccgg tttttgatac cggcattccc tggttcgaat
    21781 ccaggtaccc cagccatcga agaaataatc tggctacgta gctcagttgg ttagagcaca
    21841 tcactcataa tgatggggtc acaggttcga atcccgtcgt agccaccaaa ttctgaatgt
    21901 gtcgaatacg ttcggcaaat tcaaaaacca atttgttggg gtatcgccaa gcggtaaggc
    21961 accggattct gattccggca ttccgaggtt cgaatcctcg taccccagcc acattaaaaa
    22021 agctcgcttc ggcgagcttt ttgcttttct gcgttcattc aatgtcgaat gcgatgttga
    22081 cacgtcttat ccttcaatgt cggatgcgac gctgccgcgt cttatccgac ctacggttgg
    22141 cacgcatccg gcaatgttgt aaggctacaa ccctaacgca tatttcagcg cctgacgttt
    22201 caacacgcca gcacgctctg ccgccattaa cccgagatta cgcacaaaac gcagcggcgg
    22261 cagattatta ctgaatccgg cataaaacag atccatacca ctttgcataa tgaagttatc
    22321 cgccatgcgc cgcatctggt aacgcttgag gacaggataa ctggcccacg cttcaccgta
    22381 gctgcgggca ttcaccagaa catcaatcag ggcatcgaca tcacgataac caagattcac
    22441 cccctgcccc gccagcggat ggatggtatg cgcggcatcg cccaccagcg caagccccgg
    22501 ctgcacatac tgcaacgcat gtcggcgcgt cagcggaaac gcaccagcgg caagcggtgt
    22561 aacgtaaccc agacgcgacg ggaaatgctt cgcgatttcc gcctggagtt gcgccatatt
    22621 catattctgc aactggcgaa tacgcgccgg agagtcatac cacaccagcg atgcccagtt
    22681 atcaaacaac ggcagaaacg cacgcggtcc gtccggggta aattgctgcc aggtgctgtc
    22741 gccgggatcg ttctcacact gtacgctaat caacatgcac gactgcgcat actgccaggc
    22801 atgaacgcca attcccgcca tctgccgcac ctgagaattt gcgccgtcgg caccaatcac
    22861 cagtttcgcg cggatcgttt caccgccctt cagttccagc tcctgaagat cattatctcg
    22921 atgtaatgca atcagcgagg ttggcacacg taacgttact ttcggatgcg cttccagcgc
    22981 ctgccacaac gcctgttgca ggactgtgtt ttccaccatg tagccaagca acggcaactt
    23041 taattcggct gcgtcaaata ccacatgtgc cgtttcccac tcccaggttt ccagtctgcg
    23101 gtaaggatgg caacgcatag cctgtactgc atcccagacc cctacccctt taagcaatga
    23161 taccgaagcc gcgctgatcg ccgagatccg cacgtccggt tggctgtcag cgacaaacgg
    23221 tgcaggttct gcgtgctcga tcaccgttac cgaaaatccg tgctgtgcca gccccagcgc
    23281 cagtgcgccg ccgaccattc ctccgccgac aatggcaatt tccgttggtt gatttgtcat
    23341 ggtcaatcat cctgttagca atatgcttta agtttacagg atttttaccc ccaagggctg
    23401 ataacgctca tactggtcac aacggcagca aagcattaca ctatgcgccc tgcattcctg
    23461 gctactattt cgcaagagca agtcgatgac caaaaaactc catattaaaa cctggggctg
    23521 tcagatgaac gagtacgatt catcgaagat ggccgatctg ctggatgcca cccacggcta
    23581 tcaactgacc gacgtggcgg aagaagcgga tgtgctactg ctgaacacct gctcaatccg
    23641 cgagaaggct caggaaaaag tcttccatca gttgggtcgc tggaaactgt taaaagagaa
    23701 gaatccagac ctgattatcg gcgtcggtgg ctgcgtggca tcgcaagaag gcgagcacat
    23761 tcgccagcgc gcccactatg tcgatattat ttttgggccg caaacgctgc accgtctgcc
    23821 ggagatgatc aactccgtgc gcggcgaccg cagcccggtt gtagatatca gcttcccgga
    23881 aatcgagaag tttgaccgtc tgccggaacc gcgcgccgaa gggccgaccg cgtttgtctc
    23941 catcatggaa ggctgcaata aatattgcac ctactgcgtg gtgccttaca cccgtggtga
    24001 agaggtaagc cgtccgtctg acgatattct gtttgagatt gcccagcttg cggctcaggg
    24061 cgtgcgtgaa gtcaacctgc tcggtcagaa tgtgaatgcc tggcgtggtg agaactacga
    24121 cggcaccacc ggatcgtttg ccgatctgtt gcgtctggtt gctgcgatcg acgggatcga
    24181 tcgtattcgc tttaccacca gccatccgat cgaattcacc gacgatatca tcgaagtgta
    24241 ccgcgacacg cctgagctgg tgagcttcct gcatctgccg gtacagagcg gttccgatcg
    24301 cattctgaac ctgatgggac gtacccatac ggcgctggag tacaaagcga tcatccgtaa
    24361 actgcgtgcg gcgcgtccgg atattcagat cagttctgac ttcattgttg gcttccctgg
    24421 cgaaaccacc gaagacttcg agaaaacgat gaagctgatt gccgacgtca atttcgacat
    24481 gagctacagc tttatcttct ctgcacgtcc aggaacgcct gccgccgata tggttgatga
    24541 tgttccggaa gaagagaaga agcagcgtct gtatattctg caagaacgta tcaatcagca
    24601 agcgatggca tggagccgtc ggatgctcgg caccacccag cgtattctgg tagaagggac
    24661 ttcgcgtaag agcatcatgg agctttccgg gcgtaccgaa aataaccgcg tggtcaactt
    24721 cgagggcaca ccggatatga tcggtaaatt cgtcgatgta gaaattaccg acgtctaccc
    24781 gaactctctg cgcggtaaag tggtgcgaac tgaagatgaa atgggtctgc gcatggcaga
    24841 aacaccggag tcggtgattg cccgcacccg caaagaaaac gaccttggcg tgggttatta
    24901 tcagccgtaa ttctcaggcc tgccgttccg gtgggccttg ttttttctct ctttatcccc
    24961 atctttatcg caatgcttgc gcctttttcc tgtggcgtga ataatttcct tataggccgg
    25021 tgaatgttca gcttccggcg gcatacagat aagaggaacg gtttgaacat agacactcgc
    25081 gaaatcaccc tggagccagc agacaatgcg cgtctgttga gcctgtgcgg cccgtttgat
    25141 gacaacatca agcagctcga acgccgtctc ggcatcgaga tcaatcgccg cgataaccac
    25201 tttaaactga ccggccgtcc gatttgcgtc accgctgcgg cagacattct gcgtagcctg
    25261 tatgtcgata ctgccccgat gcgcggtcag attcaggata tcgaaccgga acagatccac
    25321 cttgcgatta aagaagcgcg ggtactggag caaagcgcgg agagcgtgcc ggagtacggc
    25381 aaagcggtca atatcaaaac taaacgcggc gtaattaagc cgcgcacgcc aaaccaggcg
    25441 cagtacatcg ccaatattct cgaccatgac attaccttcg gcgttggccc ggcgggtacg
    25501 ggtaaaacct acctggcagt ggctgcggca gttgatgccc tggagcgtca ggaaattcgc
    25561 cgtattctgc tgactcgtcc ggcggtcgaa gccggtgaga aactgggctt cctgcctggc
    25621 gatttaagcc agaaagtaga cccgtatctg cgcccactgt acgacgcgct gtttgaaatg
    25681 ctgggctttg agaaagtcga gaaactgatt gagcgcaacg ttattgaagt cgcgccgctg
    25741 gcctatatgc gtggtcgtac gctgaacgac gcgtttatca ttctcgatga gagccagaac
    25801 actaccatcg aacagatgaa gatgttcctg acccgtatcg gttttaactc aaaagcggtt
    25861 atcaccggcg acgtcacaca gatcgacctg ccgcgtaata ctaaatcagg cttacgtcat
    25921 gccattgaag tgctggccga tgtcgaagag atcagcttta acttcttcca cagcgaagac
    25981 gtggttcgtc acccggtggt ggcgcgtatc gttaacgctt atgaagccag ggaagaagca
    26041 gaacaaaaac gtaaagcggc gctggcagca gaacgcaagc gcgaagaaca ggaacaaaaa
    26101 tgagtcaggt gatcctcgat ttacaactgg catgtgaaga taattccggg ttaccggaag
    26161 agagccagtt tcaaacatgg ctgaatgcgg tgatcccgca gtttcaggaa gagtcggaag
    26221 tgactattcg catggtcgat accgccgaaa gccacagtct gaatctgacc taccgcggta
    26281 aggataagcc gaccaacgtg ctctccttcc cgtttgaagt gccgcctggc atggagatgt
    26341 cgctactggg cgatctggtt atctgccgtc aggtggttga gaaggaagct caggagcaag
    26401 gcaaaccact ggaggcgcac tgggcgcata tggtggtgca cggcagtctg catttgttag
    26461 gttacgatca cattgaagat gacgaagcag aagaaatgga agccctcgaa acagagatta
    26521 tgcttgctct gggctatgag gatccgtaca ttgccgagaa agaataattt gccaggcatt
    26581 tgactggcag acgtccccgc cgttggtgat taacgacggc gttttgatta actaacatga
    26641 gaacccatac gacgccatga gcgacgacaa ttcacacagt agtgacacga taagcaacaa
    26701 gaagggattt ttctccctgt tactcagcca acttttccac ggtgaaccga aaaaccgtga
    26761 cgaactgctg gcgctgatcc gtgattccgg gcagaacgac cttatcgacg aagatacgcg
    26821 cgatatgctc gaaggggtga tggacatcgc agaccaacgc gttcgcgaca tcatgatccc
    26881 ccgctcccag atgattaccc tgaaacgcaa ccagacgctg gacgaatgcc ttgatgtcat
    26941 catcgagtcc gcccactcac gtttcccggt gattagcgaa gacaaagatc acattgaagg
    27001 gattctgatg gcgaaagact tgctgccgtt tatgcgcagc gatgctgaag ccttcggcat
    27061 ggacaaagtg ttacgtcagg cggttgtcgt tcctgaaagt aagcgcgtag accggatgct
    27121 gaaagagttt cgctctcagc gttaccacat ggcgatcgtt attgacgaat tcggtggggt
    27181 ttccggtctg gtgaccattg aagacatcct ggaactgatt gttggtgaga ttgaagacga
    27241 gtatgacgaa gaagatgata tcgacttccg tcagctgagt cgtcatacct ggaccgtgcg
    27301 cgcactggct tccattgaag acttcaacga agcgttcggc acccacttta gcgatgaaga
    27361 agtcgacact atcggtggtc tggtgatgca ggcgtttggg catcttccgg cgcgtggcga
    27421 aactatcgac atcgacggtt accagttcaa agtggcgatg gccgatagtc ggcgtattat
    27481 tcaggttcat gtcaaaatcc cggatgactc accccagcca aagctggatg aataaaaccg
    27541 aaactggata gataactaca tggcttttgc ctcattaatt gaacgccagc gcattcgcct
    27601 gctgctggcg ttattattcg gtgcctgcgg aacgctggcc ttctctcctt acgacgtctg
    27661 gcctgcggcg attatttcgc tgatggggct tcaggcgttg acctttaacc gtcgcccact
    27721 ccagtctgcc gctattggct tttgctgggg atttggcctc tttggcagcg gtattaactg
    27781 ggtctatgtc agcatcgcga cctttggcgg gatgcctggc ccggttaaca tcttcctggt
    27841 ggtgctgctg gcggcgtatt tgtcgctgta taccggactg tttgctggcg tgctgtcgcg
    27901 tctgtggccg aaaaccacct ggctgcgcgt ggcgattgcc gcccctgctc tctggcaagt
    27961 gaccgagttt ctgcgcggtt gggtgctgac tggcttcccg tggttacagt tcggctatag
    28021 ccagattgat ggcccgttaa aagggctggc accgctaatg ggcgtggaag ccattaactt
    28081 cctgctgatg atggttagtg gcctgctggc actggcgttg gtcaaacgca actggcgtcc
    28141 gctggtggtg gccgtcgtgc tgtttgccct acccttcccg ctgcgttaca tccaatggtt
    28201 taccccgcaa ccggagaaaa ccattcaggt ttcgatggtt cagggcgata ttccgcaatc
    28261 gctgaaatgg gacgaaggcc agcttcttaa tacgctaaag atttactaca acgcaacggc
    28321 accgctgatg ggcaaatcat cgttgattat ctggccggag tcggcgataa ccgatctgga
    28381 aattaatcag caaccgttcc tcaaagcact ggacggtgag ttgcgtgata aaggtagctc
    28441 gctggtaacc gggattgtcg acgcgcgtct caataagcag aaccgctacg atacctacaa
    28501 caccatcatc acgctgggta aaggtgcacc gtacagctac gaatcagccg atcgctataa
    28561 caaaaaccat ctggtgccgt ttggcgagtt tgtcccgctg gagtcgattc tgcgtccgtt
    28621 agcaccgttc tttgatctgc cgatgtcgtc gttcagccgt gggccatata tccagccgcc
    28681 gctgtcggca aatggtattg agcttactgc ggctatttgc tacgagatca ttctcggcga
    28741 gcaagtgcgc gataacttcc gcccggatac cgactacctg ctgactatct ccaacgatgc
    28801 gtggtttggt aaatctattg gtccatggca acacttccag atggcgcgaa tgcgtgcgct
    28861 ggagctggcg cgcccactgt tgcgcagcac caacaacggc attacggcgg tgattggccc
    28921 gcagggtgag attcaggcga tgatcccgca gttcacccgc gaggtgttaa ccactaacgt
    28981 gacgccgacc accggactca caccatacgc acgtaccggc aactggccgc tgtgggtgct
    29041 gacggcactg tttggttttg ctgctgtgtt gatgagtctg cgtcagcgac gtaaataatc
    29101 ccttcttatc gtgccggaat acatcccata tttcggcacg ctcaccggat gcggcatcag
    29161 tgcattatcc ggataacaat attctcctat ccaactttgg cacatctatt gctttgttat
    29221 acaaggcaaa gcttgaacca gcatcagtac aacccggttg caccacagca atccaccggt
    29281 agcaccaaaa gagtgcattt taagaatttt gcttcagaat ggtgcggtgc acctcgcaaa
    29341 aacaaacaat aaaacatcaa tatctttaca tttagccaaa ctaaatgtta cctaacaggc
    29401 acaacactgc acaataaagt tgcagacgat aacaacacaa acactcacaa cgggtatcca
    29461 tgcgttctta acgcagaaga taaaggagtt ggatatgcaa ttacgtaaac ctgccacagc
    29521 aatcctcgcc ctggcgcttt ccgcaggact ggcacaggca gatgacgccg ccccggcagc
    29581 aggcagtacg ctggacaaaa tcgccaaaaa cggtgtgatt gtcgtcggtc accgtgaatc
    29641 ttcagtacct ttctcttatt acgacaatca gcaaaaagtg gtgggttact cgcaggatta
    29701 ctccaacgcc attgttgaag cagtgaaaaa gaaactcaac aaaccggact tgcaggtaaa
    29761 actgattccg attacctcac aaaccgtatt ccactgctgc aaaacggcac tttcgatttt
    29821 gaatgtggtt ctaccaccaa caacgtcgaa cgccaaaaac aggcggcttt ctctgacact
    29881 attttcgtgg tcggtacgcg cctgttgacc aaaaagggcg gcgatatcaa agattttgcc
    29941 gacctgaaag gcaaagccgt agtcgtcact tccggcacta cctctgaagt tttgctcaac
    30001 aaactgaatg aagagcaaaa aatgaatatg cgcatcatca gcgccaaaga tcacggtgac
    30061 tctttccgca ccctggaaag cggtcgtgcc gttgccttta tgatggatga cgctctgctg
    30121 gccggtgaac gtgcgaaagc gaagaaacca gacaactggg atatcgtcgg caagccgcag
    30181 tctcaggaag cctacggttg catgttgcgt aaagacgatc cgcagttcaa aaagctgatg
    30241 gatgacacta tcgctcaggt gcagacctcc ggtgaagcgg aaaaatggtt tgataaatgg
    30301 ttcaaaaatc caattccgcc gaaaaacctg aacatgaatt tcgaactgtc agacgaaatg
    30361 aaagcactgt tcaaagaacc gaatgacaag gcactgaact aattacaaga accaggggcg
    30421 gaaattccag ccctctcgat tgttacgtag cacggacaga ctatacgcct gatggtcgtt
    30481 ccccatcggg cctgaaaacc gcaatacgct gggtaacaat cttcgagggt agcagttaac
    30541 gctgctaccc tcttttttct ggagtagatt tatgtctata gactggaact ggggtatttt
    30601 tttacaacaa gccccgttcg gcaataccac ctatcttggt tgggtctgga gtggttttca
    30661 ggtgacgatc gctttgtcga tctgcgcctg gattatcgct ttcctcgtcg gttcattttt
    30721 cggcatttta cgtaccgttc caaaccgttt tctctccggt ctgggtacgt tgtatgtcga
    30781 actgttccgc aacgtgccgt tgattgtgca attctttacc tggtatctgg tgatcccgga
    30841 gctgctgccg gagaaaatcg gcatgtggtt taaggccgag ctggatccca atattcagtt
    30901 tttcctttca tccatgctct gcctggggct gtttaccgcc tcccgtgttt gcgaacaggt
    30961 acgcgccgct attcagtcgc taccgcgtgg gcagaagaat gccgcgctgg cgatgggcct
    31021 gacgctaccg cagacttatc gatacgtact gctgcctaat gcttatcgcg ttatcgtccc
    31081 gccgatgact tccgagatga tgaacctggt gaaaaactcc gccatcgcct cgaccatcgg
    31141 tctggtggat atggcggcgc aggcgggtaa attgctcgac tactcggctc acgcctggga
    31201 gtcgtttacc gccatcacgc ttacgtatgt gttgattaac gcttttatca tgctggtgat
    31261 gacgctggtt gaacgtaaag ttcgcctgcc cggcaacatg gggggcaaat aatgtacgag
    31321 tttgactgga gttccattgt tccttccctg ccatatctgc tcgacgggct ggtgatcacc
    31381 ctgaaaatca ccgtcacggc agtcgtgatt ggtattttgt ggggtaccat gctggcggtg
    31441 atgcgcttgt ccagcttcgc gcctgtttcg tggtttgcca aagcctacgt taacgtattc
    31501 cgctcgattc ctttagtcat ggttttgctg tggttttact tgattgtgcc gggatttctg
    31561 caaaatgtgc tgggattatc gccaaaaaat gatattcgtt taatttcggc gatggtggcg
    31621 ttttcgatgt ttgaagcggc ctactattca gagattatcc gtgccggtat tcaaagtatt
    31681 tcccgtggtc agtcgagcgc cgcgctggcg ttggggatga ctcactggca gtcgatgaaa
    31741 ctgattattc tgccgcaggc tttccgcgcg atggtgccgc tgctgctcac tcagggcatc
    31801 gtactgttcc aggatacctc gctggtgtat gtgttatctc tggccgattt cttccgtacc
    31861 gcctcaacca ttggtgagcg tgatggtacg caggtcgaga tgatcctgtt tgccggattt
    31921 gtttatttcg ttattagcct tagcgcgtcg ttgttggtca gctacttgaa aagaaggaca
    31981 gcataatgat taccctgaaa aatgtttcaa aatggtatgg tcactttcag gtgctgaccg
    32041 actgctcaac cgaagtgaaa aaaggcgaag tggtggtggt ttgcggcccg tctggttccg
    32101 gcaaatcaac gctgattaaa accgtcaacg gcctcgaacc agtgcagcaa ggtgaaatca
    32161 ccgtcgatgg tatcgtggtt aacgacaaga aaaccgatct ggcaaagctg cgctcccgcg
    32221 tcgggatggt attccagcat ttcgagctgt tccctcatct gtcgattatc gaaaacctga
    32281 ccctggcgca ggtgaaagtg cttaaacgcg ataaagcgcc cgctcgtgaa aaagcattga
    32341 aattgctgga gcgcgtggga ctttccgcac atgccaataa atttccggcg cagctttccg
    32401 gcggtcagca gcagcgtgtg gcaatcgctc gcgcattgtg tatggctcct attgcgatgc
    32461 tgttcgacga accgacatcg gcgctggatc cggagatgat caacgaagtg ctggacgtga
    32521 tggtggaact ggcgaacgaa ggaatgacca tgatggtggt gacccacgaa atgggctttg
    32581 cccgcaaagt ggcgaatcgg gtgatcttta tggacgaggg taaaattgtc gaagactcgc
    32641 cgaaagacgc tttcttcgat gatccgaaat cggaccgcgc aaaagacttc ctcgcgaaaa
    32701 tcctgcatta atcactctgg cgcgcatacc caattgcgcg ccatctcacg ctttatcgtt
    32761 tcgcccctcg tatctgttac atcctggcgt tacccttgtc gcaaagaagc acacaacaag
    32821 gagcaacaat ggcactgcca attctgttag attgcgaccc aggtcatgat gacgctatcg
    32881 caatagttct cgccctcgcc tcaccagagc ttgatgtcaa agcaattacg tcttccgccg
    32941 gaaaccagac accagaaaaa accttacgca atgttctgcg tatgctgacc ttgcttaatc
    33001 gcaccgatat tccggtggca ggcggcgcgg taaaaccgtt aatgcgtgag ttgattatcg
    33061 cggacaatgt gcacggcgaa agcggtctcg acggcccggc attaccggaa ccgacgttcg
    33121 caccgcaaaa ctgtacggcg gtggagctga tggcgaaaac gctgcgcgaa agtgcggaac
    33181 ctgtcacgat tgtttctacc ggaccgcaaa ctaacgttgc cttgctgctc aatagccatc
    33241 cggaactgca tagcaaaatt gcccgtatcg tgattatggg cggcgcaatg gggcttggta
    33301 actggacgcc tgcggctgaa tttaatattt acgttgaccc ggaagcggca gaaattgtct
    33361 tccagtcagg gatcccggtg gtgatggccg gtctggatgt tactcataaa gcgcaaatcc
    33421 acgttgaaga caccgagcgt ttccgcgcga ttggcaaccc tgtttcaacc attgttgccg
    33481 aactgctgga tttcttcctc gaatatcata aagacgaaaa atggggcttt gtcggcgcac
    33541 cgctgcatga cccatgcacc atcgcctggc tgttgaaacc cgagttgttt accactgttg
    33601 agcgctgggt tggcgtggaa acacagggga aatataccct gggtatgacg gttgttgatt
    33661 attattatct gactggcaat aaaccgaatg ccactgtaat tgtcgatgtt gatcgtcagg
    33721 gctttgttga tttactggct gatcgtctga aattttacgc ttaacactcg tcattgatta
    33781 agatcaccac gcgttacctg ggtggtctta atctatttat ctctttttct ttcaagatat
    33841 aaatgcaatg gataatgcag aactcgccat aggtatcgat ctcggcacta ccaatagttt
    33901 aattgccgtc tggaaagacg gtgccgcgca attaattcca aataagttcg gtgaatattt
    33961 aacaccatcc ataattagca tggatgaaaa taatcatatt ttagtcggaa aaccggctgt
    34021 atcacggcgt acttcgcatc cggataaaac ggcagcgtta tttaaacgtg caatgggcag
    34081 taataccaac tggcggttag gcagcgacac atttaacgcg ccagaactgt cctctttggt
    34141 attacgctca ttaaaagaag atgccgaaga atttctgcaa cgtccgatta aagatgtggt
    34201 gatctccgtt ccggcttatt tcagcgataa acaacgcaag catacccgtt tagcagcgga
    34261 cttagccggg ttaaatgcgg tacgcttaat taatgaaccc acagcagctg cgatggcgta
    34321 tggcctgcat acccaacaaa atacccgttc gctggtgttt gatctcggtg gcggcacgtt
    34381 tgacgttacg gtgcttgagt acgccacgcc ggtgattgaa gttcacgcct ccgctagcga
    34441 caacttcctc ggtggcgaag attttaccca tatgttggtc gatgacgttt taaagcgcgc
    34501 ggatgtcaca aggaccacgc ttaacgagag tgaactggca gccttgtacg cctgtgtgga
    34561 agcggcaaaa tgtagcaatc aatcgccaat gcacattcgc tggcagtacc aggatgaaac
    34621 gcgggaatgc gaattttacg agaacgaact ggaagatttg tggttgccgc tgctcaatcg
    34681 cttgcgagtg ccgattgaac aggcgttgcg cgatgcgcgt ctgaagccga gtcaaatcga
    34741 cagtctggtg ctggttggcg gcgcgtcaca aatgccgctg gtgcagcgaa tcgccgtgcg
    34801 tctgtttggc aaattaccgt atcaaagtta cgatccgagc accattgtcg cgctgggcgc
    34861 agcaatccag gccgcctgcc gcttacgcag tgaagatatt gaagaagtga tcctcactga
    34921 tatttgccct tactcgttgg gcgttgaggt taaccgccag ggcgtttccg gcattttctc
    34981 gccgattatt gaacgaaaca ccactgtgcc cgtgtcgcgt gtagaaactt attcaaccat
    35041 gcacccggaa caggattcaa ttacggttaa cgtctatcag ggagaaaacc acaaagttaa
    35101 aaacaacatt ctggtggaat ccttcgatgt gccgttgaag aaaaccgggg cttatcagtc
    35161 gattgatatt cgctttagtt atgatatcaa cgggttgctt gaagttgacg tgcttctgga
    35221 agacggcagc gttaagtcca gagtgattaa ccacagcccg gtaacattga gcgcgcagca
    35281 gattgaagag agtcggacgc ggttatccgc attgaaaatt tatccgcgcg atatgctcat
    35341 caatcgcacc tttaaagcca aactggaaga gttgtgggcg cgggcgctgg gtgacgagcg
    35401 agaagagatt ggccgggtga tcaccgattt tgatgcggcg ctgcagtcaa acgatatggc
    35461 ccgcgtcgat gaggtcaggc ggcgggcgag cgtttattta gccattgaga cctcataatc
    35521 acggctgccg gatgcggcgt aaatgctgca tccggcatcg gcgcaaaaaa cacatcgtca
    35581 actaaacaac atcgcgattt tgccgagagc ataacctata accagcaccg ttaaactgat
    35641 tttgataatg ctggtaatgc tctgtggttt ttcaccctca tcattttttt cttcatggtt
    35701 ttcaggtgtt ggctcatcga catcttcatc ctccggctcg agttttactt tcgccaacag
    35761 catccggttg ataatggcct ctaatggatg agcatctccg ccttccagcc ttgtattaag
    35821 acgggccagc atctcttgtt tcggaaacgc ttcaagtact acctgttccg ctacccgata
    35881 caaatccagt aacggtgaat cttcttctgt ctcgctaccg actctcgatt tccaccacgc
    35941 ccatgctttg cgtttgaaga ataacgactc aataaacgat tgacctaacg gcgataattt
    36001 tccgctatgt aaaatttccg cccataacag ccgcgtccgg gcgctctcac caggaacata
    36061 tttcagcggt tgaccttgcg catctaccat gctccgcgct tcgataacca tcactaataa
    36121 cgcgaaataa tccgggcaat gacgcttgct ccactgcaac aacaaattat cagcttgtgt
    36181 agatgggtaa ctttcccagt acgcgccgag atcagcaagc aggctttccc cctcgccgca
    36241 gtacaaacgt tgtgcacact ggtaataccg ggcatcttcg ctttctggtt cttccgcttg
    36301 ccactgtttg gctacaggaa tgagttccac catacctgtt tgcaccgagc tgtaccagcg
    36361 caacagtttg cgatgtaact ttgcatcatc aggaatgaac cacggtcctt ccatcgccaa
    36421 aaacgcagtg acatagtcag gatggtaacg gcagcaacgt tccagcgcga agtaatattc
    36481 aacagtctgg ttctgtaacg cgacgggata ttccagaagg ctaaggaaat cgaaacaatc
    36541 accagcctga atggcctcaa gaaaagcctc cctttcttcc tcatccattc cttcgctgtc
    36601 attaaacgaa tgccagttca aacgctctgc caacaaagaa gcacaattta atgacagata
    36661 tcgcgcttct atagcgattg cacacagcgg ccagcgtaat tgatcgacat cctccatgtt
    36721 ccaggtattt aattgctgaa taaatttctg ccaagcggag ggctgaaaac gatcactttc
    36781 tgaatccagt aatgtcctga atgcacgtag tatttcatgt tcagcggtgg catctttttc
    36841 ttcatcatct gcttcctcga caggatttac cgccagtcgc agcgcctctt cataggcctg
    36901 gcgtaatgct ttaaacccct gcggatcggt ttcgggatga cacaacggta agcgagcaag
    36961 ataagcctgg cggataatgt ctatttgcgt cgtgctttca atttcgagga tttgccaaca
    37021 tgttttcata ggtcgctcac atccttaagc gttaccgggc attcaggctg gacagttaat
    37081 ggctgccagg gaagcgtggc atagaaacgg tcgcttcccg ctgccagctc ttcgaacatc
    37141 cgactcccgg cgctatcgta tttgtacagg gttagcgcct cgcgtagcga agcgttgtct
    37201 tcgcactgag aagattgcca gtagagacgc cctagtgaat aggcagaaaa atagtgcatc
    37261 cagctgtgat agtaatggtg agctcgtgca tagacgcgcg attgcagcca taaactctct
    37321 tcttccgtca gccagttgtt gagcacaccg gcccgtgaca gaaaccccat tcgaacataa
    37381 tcccaggctt tgattccccc ttctccacag cacatagcgg tttgggcaac gaattgggta
    37441 taaatcaacc ctctctccga gccaccagca attaatgctt tccactcttc aggggaagag
    37501 cgttgccatg tcagatataa gccagctaaa tcattggcat gaccatcatc agtcatctga
    37561 taaatagtct gaattaaatc atcacgatta tttattcccc aggatgatga gagatcgaat
    37621 tcttttatcc cggtatacat ttttggcgag tcatgcgcgg gtttgcaact ctgattaagt
    37681 atttccatgg gcgcagaaag cgcaaaaaga agatattgtt cttccttatt cattttttaa
    37741 taatccctta agtgcattaa ctgactactt ttttattatt gatgagtata aacttgacgc
    37801 tttcaaaata aaaagaaaat cgaagcattc acacatgaat aaaaaattaa tgtatatatt
    37861 cgcaatttt
//
LOCUS       DAETPZ010000030        37626 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.030, whole genome shotgun sequence.
ACCESSION   DAETPZ010000030 DAETPZ010000000
VERSION     DAETPZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37626
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.030"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..175)
                     /locus_tag="K0W59_001764"
     CDS             complement(<1..175)
                     /locus_tag="K0W59_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HBI3022830.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQYR"
     gene            complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="K0W59_001765"
     CDS             complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="K0W59_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBI3022831.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     YYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1229..1525)
                     /gene="fis"
                     /locus_tag="K0W59_001766"
     CDS             complement(1229..1525)
                     /gene="fis"
                     /locus_tag="K0W59_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HBI3022832.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="K0W59_001767"
     CDS             complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="K0W59_001767"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBI3022833.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="K0W59_001768"
     CDS             complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="K0W59_001768"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBI3022834.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKTEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNAVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3738..5189)
                     /gene="panF"
                     /locus_tag="K0W59_001769"
     CDS             complement(3738..5189)
                     /gene="panF"
                     /locus_tag="K0W59_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HBI3022835.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5179..5421)
                     /locus_tag="K0W59_001770"
     CDS             complement(5179..5421)
                     /locus_tag="K0W59_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HBI3022836.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5530..6879)
                     /gene="accC"
                     /locus_tag="K0W59_001771"
     CDS             complement(5530..6879)
                     /gene="accC"
                     /locus_tag="K0W59_001771"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBI3022837.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVQSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNTIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6890..7360)
                     /gene="accB"
                     /locus_tag="K0W59_001772"
                     /gene_synonym="fabE"
     CDS             complement(6890..7360)
                     /gene="accB"
                     /locus_tag="K0W59_001772"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBI3022838.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8338..9312)
                     /locus_tag="K0W59_001773"
     CDS             complement(8338..9312)
                     /locus_tag="K0W59_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBI3022839.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPSTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9464..11404
                     /gene="csrD"
                     /locus_tag="K0W59_001774"
     CDS             9464..11404
                     /gene="csrD"
                     /locus_tag="K0W59_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HBI3022840.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVIEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11709..12752
                     /gene="mreB"
                     /locus_tag="K0W59_001775"
     CDS             11709..12752
                     /gene="mreB"
                     /locus_tag="K0W59_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HBI3022841.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12818..13921
                     /gene="mreC"
                     /locus_tag="K0W59_001776"
     CDS             12818..13921
                     /gene="mreC"
                     /locus_tag="K0W59_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBI3022842.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13921..14409
                     /gene="mreD"
                     /locus_tag="K0W59_001777"
     CDS             13921..14409
                     /gene="mreD"
                     /locus_tag="K0W59_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBI3022843.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14418..15011
                     /gene="yhdE"
                     /locus_tag="K0W59_001778"
     CDS             14418..15011
                     /gene="yhdE"
                     /locus_tag="K0W59_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HBI3022844.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15001..16470
                     /gene="rng"
                     /locus_tag="K0W59_001779"
     CDS             15001..16470
                     /gene="rng"
                     /locus_tag="K0W59_001779"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HBI3022845.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16538..20338
                     /gene="yhdP"
                     /locus_tag="K0W59_001780"
     CDS             16538..20338
                     /gene="yhdP"
                     /locus_tag="K0W59_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HBI3022846.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QSAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKAR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20494..21939
                     /gene="tldD"
                     /locus_tag="K0W59_001781"
     CDS             20494..21939
                     /gene="tldD"
                     /locus_tag="K0W59_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HBI3022847.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="K0W59_001782"
     CDS             complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="K0W59_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HBI3022848.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23179..23382
                     /locus_tag="K0W59_001783"
     CDS             23179..23382
                     /locus_tag="K0W59_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HBI3022849.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23390..24322
                     /gene="aaeA"
                     /locus_tag="K0W59_001784"
     CDS             23390..24322
                     /gene="aaeA"
                     /locus_tag="K0W59_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HBI3022850.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24328..26295
                     /gene="aaeB"
                     /locus_tag="K0W59_001785"
     CDS             24328..26295
                     /gene="aaeB"
                     /locus_tag="K0W59_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HBI3022851.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGMLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26387..26659
                     /locus_tag="K0W59_001786"
     CDS             26387..26659
                     /locus_tag="K0W59_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HBI3022852.1"
                     /translation="MNIYAFDFNEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="K0W59_001787"
     CDS             complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="K0W59_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HBI3022853.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27344..27814)
                     /gene="argR"
                     /locus_tag="K0W59_001788"
     CDS             complement(27344..27814)
                     /gene="argR"
                     /locus_tag="K0W59_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HBI3022854.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28249..29187
                     /gene="mdh"
                     /locus_tag="K0W59_001789"
     CDS             28249..29187
                     /gene="mdh"
                     /locus_tag="K0W59_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBI3022855.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(29249..30316)
                     /gene="degS"
                     /locus_tag="K0W59_001790"
     CDS             complement(29249..30316)
                     /gene="degS"
                     /locus_tag="K0W59_001790"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HBI3022856.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30406..31773)
                     /gene="degQ"
                     /locus_tag="K0W59_001791"
     CDS             complement(30406..31773)
                     /gene="degQ"
                     /locus_tag="K0W59_001791"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HBI3022857.1"
                     /translation="MKKQTQLLSALALSAGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPSSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(31927..32325)
                     /locus_tag="K0W59_001792"
     CDS             complement(31927..32325)
                     /locus_tag="K0W59_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HBI3022858.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            32519..33646
                     /gene="zapE"
                     /locus_tag="K0W59_001793"
     CDS             32519..33646
                     /gene="zapE"
                     /locus_tag="K0W59_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HBI3022859.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33865..34293
                     /gene="rplM"
                     /locus_tag="K0W59_001794"
     CDS             33865..34293
                     /gene="rplM"
                     /locus_tag="K0W59_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBI3022860.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34309..34701
                     /gene="rpsI"
                     /locus_tag="K0W59_001795"
     CDS             34309..34701
                     /gene="rpsI"
                     /locus_tag="K0W59_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBI3022861.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35096..35734
                     /gene="sspA"
                     /locus_tag="K0W59_001796"
     CDS             35096..35734
                     /gene="sspA"
                     /locus_tag="K0W59_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HBI3022862.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLINTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35740..36237
                     /gene="sspB"
                     /locus_tag="K0W59_001797"
     CDS             35740..36237
                     /gene="sspB"
                     /locus_tag="K0W59_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HBI3022863.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36280..37626)
                     /gene="dcuC"
                     /locus_tag="K0W59_001798"
                     /pseudo
     CDS             complement(36280..37626)
                     /gene="dcuC"
                     /locus_tag="K0W59_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
BASE COUNT         9109 a         9412 c         9904 g         9201 t
ORIGIN      
        1 ttctgtactg aaaattcagt tcgtcacagt taaccagttt tagtggatta actgagttca
       61 tattacggca tttgtctgtt tctgttgtca gcagttcaat ccactgcatc agtaaagtat
      121 caaacagaaa aacgaagaca gcgaaaacga cgagccacca atacttgcga atcattacct
      181 gagcctgtgc atgtttaacc aagtctggca ggaaatatac acgaaatgaa taatctgaaa
      241 accaggctta cacttccttt actttgcaat gagatcgggg tcaacttcgc ttaaccggct
      301 tcgttttgac aggttgccgg tctttctttt tttcactttc gccagttctt ccgcggagta
      361 atgcgacgcg acatccagcc gtcgaagccc cattttgatg tactcgctgt tgatctcaat
      421 accaatgaat tttcgtccgc tggcgatggc tacggcaccg gtagtaaagc taccggcaaa
      481 cgggtcgaga acgatatcgc ctgggttgga agaggcgaga ataatgcgtt tcagtaaggc
      541 tttcggtttt tgcgtcgggt ggttctcata ttcatccatc aaataacgca cgcgcggaaa
      601 atcccagacg ttacccggta ctttttgatg attgtatggc tgtggaggat ttttgcgata
      661 atagatcaac gcgcgctgcg atccggtttt ggcttctacc agaatagcat caccgttgaa
      721 tgtgtagttc tttgcgtctt tcaccatcat caggatgggt tcgtacatgg agccgtagtg
      781 ttttttcgcc tgtactccag aactgtcata tgaccagacg atgcgacttt tgatggtaaa
      841 aagcttgcgg cactggagat cgataaaggg catgttttcc gtactgttca taatgtacat
      901 gctgccctgc tttttcagaa cgcggtggca ctctgcaatc acttcaaaca gccagtcgat
      961 aaacagatct tctttccagg cttcgatcag accatcaaaa tttttaccga tgttatacgg
     1021 tgggtcggca aagagcagat cgacgctttc agtaggtagc tttttaagtt cggcaagggc
     1081 atcaccgtga ataatggtct tagcttcatt accaaaccgg gtcggttcac atcctgttct
     1141 catggtcact ccctttgtga cacctataaa aaaggcgctt ccctatgccg agtagcgcct
     1201 ttttaatcaa gcatttagct aacctgaatt agttcatgcc gtattttttc aattttttac
     1261 gcagcgtacc acggttgatg cccatcatca gcgcagcacg ggtctggtta ccacgggtgt
     1321 attgcatcac catgtccaac aggggctgtt ctacttcagc cagtaccagc tcatagaggt
     1381 cattcacatc ctgaccattc agttgagcaa aatagttctt cagtgcctgt ttaaccgagt
     1441 cacgcagggg tttttgggtt acctgatcct gagagttaac ggtagaaacg gtcagtacgt
     1501 cagaatttac gcgttgttcg aacatagttc tgtcagctct ttatttctgt ttacgcaaaa
     1561 ttttcgaagt atgcctccaa cgcctccagc tgttcgctgg catcctcaat ggcgttgaat
     1621 gtgcgccgaa actggtcatt tggagcgtgt tcctggagat accaggaaac gtgtttacgt
     1681 gcaattcggt acccttttgc cggaccataa aagtcatgca gttcccgaac gtgcgcgcaa
     1741 agcaagcgct taacctctgc caaaggcagc gggggcagca actccccagt gtccagataa
     1801 tgctggattt cccgaaagat ccagggtctt ccctgagctg cgcggcctat catcagggca
     1861 tccgcccctg tatagtcgag cacagctctg gctttaagcg ggtcagtaat gtcgccattc
     1921 gcgataaccg gaatggaaac tttctgctta actgcccgaa tactgtcgta ctcagcttct
     1981 ccattgaaca aacaggcgcg tgtacggcca tgaatggtca gagcctgaat gccacagtct
     2041 tcagccagtt gggcaatctc ttcgcagtta cggtgttccg gtgcccagcc ggtgcgaatc
     2101 ttcagggtaa caggaacgtc cactgcattg acgacctcgg taaggatcga tttaacgaca
     2161 tccgggtact gcaagagggc tgaacctgcg agcttgcgat tcactttttt agccgggcaa
     2221 cccatattga tatcaataat ctgggcaccg ctttccacgt taatacgtgc tgcatctgcc
     2281 atttctttcg gagcgctacc agcaatttgc acggtgcgaa taccgggttc atcaatgtgc
     2341 accatccgta aacgagattt gtcgctttcc caaacctgtg ggttagaaga catcatctcg
     2401 gatactgtca atccggctcc catctcgtag cacaacgtcc gaaaaggtct gtctgtaatg
     2461 ccagccatgg gcgctgcgat caggcgattt ctgagctgat attgtccgat gcgcatgagt
     2521 taagaaatga ccatactgtg actgcaaggc ggcgtatatt acgcattttt tgcacgagat
     2581 gaaaggccaa actttgacca atcctctgag atggatcaaa gaattgcatt taaaatgagc
     2641 gtggtgcgat aattactcat aaaaatcatc atattagaaa atagtgacta aaatttacac
     2701 tcaaagaaat ttgagtaagt tctcaatttt tctttatgaa tgaaaatttt ggcacgcaaa
     2761 tttgcgtaaa taatcggcaa atttacgtgc cttttgtgag cttgctcgca cttcgccccg
     2821 cgtcacccta cggcgatgcg aaggttaatt cttacgaccg gtaatacggc accactcttc
     2881 tttttccacg accgggtcca gtgcgaagct atcggcataa gcttcacaaa cgctctctgc
     2941 ctggcttgcc agaataccgg aaaggcccag caaaccgcct gaaaccggca ggacgctgat
     3001 taacggtgcc agttcacgta atgggcctgc aaggatgtta gcgaccacca cgtcggcttt
     3061 catttcttct ggctgatctt tcggtaagta gagttccaga cggtcagaaa cggcattacg
     3121 ttcggcgtta tcgcggctgg cctgaatcgc ctgcggatcg atatcaatac caatggcttt
     3181 tgctgcaccc agtttcagcg ccgcgatcgc cagaatgccg gaaccacagc caaagtcgat
     3241 gactgtttta ccggttaaat cgaggctgtc gagccattgc aggcacagag aggtggttgg
     3301 atgggtaccc gtaccaaacg ccagccctgg atctaacatc acgttgacgg cgttttcgtc
     3361 cggcacatca cgccagctag ggcagatcca cagtcgttca ccaaagcgca tcgggtggaa
     3421 attatccatc cattcgcgct cccagtcttt atcttctagt tgttcggttt tatgcgcgaa
     3481 gcctgcgccg agcagcggat ggttttccag aatcgccacc acgtcgttca tatcggtttc
     3541 agcgtcgaac agaccaatca catcggtgtc gccccacagg cgcgtttcgc ccggcagcgg
     3601 ctcaaatact ggcgtatcgt gggtatcctg aaaagtgata gaaacggcac ccgcttccat
     3661 cagcgcatcg ctaagatctt ccgcgttcgc gccggtggtg ttcagtttca gttggatcca
     3721 aggcatggca aaactcttta tttatcagta gtcaaaacgg tagcttgcgg gacggatgta
     3781 ccgaaacggt ttccgaccag gaaagccagc aaacttagta gtaacgaggg cacgataggg
     3841 tggaagccca ggtactgaat attcagcgtc gcgagtacgg catacagcac gccgccaacg
     3901 atcatcgcac ttagcgcgcc tttggcgttg gcgcgttccc agtaaagacc cagcaccagc
     3961 ggccacagga aaacggcttc cagcccaccg aaggccagca aattcagcca gatgatcatt
     4021 tctggcggct tccaggcggc aagcagcagc aacgcgccga gaactaacgt aattaccgcc
     4081 gacatccgct tcagacgcgt ctcgttttgc atttgatccg gacggatatt cagatagaga
     4141 tctttaatga tcgtagcgga actttgcagc agttgggcgt taattgtcga catgatcgca
     4201 gccataggtg cagccagaaa gatcccggca gcaaaaggtg gcagcacttt taccattaac
     4261 gtcgggatca ccagatccgg tacggtgaga tcggggatca ctgcacgacc taacgctccg
     4321 gctaggtgca taccgaacat caggattgcg actacaatcg taccgatgat gatcccccgg
     4381 tgtactgctt tgctgtcttt ataagagata cagcgcaccg cagtatgcgg caggccaatc
     4441 acgccaaagc acaccagtac ccagaacgac gtcataaagg caggcgacag aatatcgtca
     4501 gcgccttgtg gcgtaaccag ttgcggatcg atggtttgca aggtctgtac tgcgttgctt
     4561 aagccgccag cggcatgtac cacgccaata agcagcacaa cggtgccaat cagcatcaca
     4621 agcccttgca tggtgtcgtt cagcacgctg gcgcgaaagc caccaaaggc ggtatataac
     4681 gcaatgctga taccaaaaat cagcagcccg gtttcataag gaatacccgc cgcggtttcc
     4741 agcaggcgcg caccgccgat aaactgcacg gtcatcgcac caacgaacgc aaccagcaaa
     4801 ctcaaactcg ccagccacac cagaagacga ctctggtagc gggcaaacag catatcgttc
     4861 agcgtcactg cattgtagcg gcgcgcaaga atcgcaaact tcttgccgag aataccgagt
     4921 gaaagccaga cagcaggaag ctgaatcatc gccagcaata cccagcccag cccgtattta
     4981 taagcagctc ctggcccgcc gataaacgaa ctggcactga tataggtcgc ggtgagcgtc
     5041 atcgccagca caataccgcc catagagcgg ctgccgagga aatactcatt aaggaaggtg
     5101 ccggtgctcc gtttacgcat cgcataaacc gagataccga acaccaccac cagataggcg
     5161 accagcggta gaattacttc aagctgcatc gtcatcctcc agtgggatat cgcgatagat
     5221 aaatttcacc atcgcccagc acagtccaat aaacagtagc ggcgtcagga tgcaggccat
     5281 ctcaaaccag cgcggaaagc cggtaaaacc gggggcaacg ccaggtaagt aagcatctac
     5341 taaccaaact gccagataca aaagggtcag ccccagcgcc cagcgcgcct ctttatgggc
     5401 ctgaacaaaa cgagtgtcca ttttttgtcc ctgatgggtg aagaaagcga ggattgtacc
     5461 ttatgggggt tgtcgatccc cagtaataaa aaaggccgga aaatccggcc ttttgacgct
     5521 tttgcagtct tatttttcct gaagaccgag ttttttctcc agatagtgga tgttagtgcc
     5581 accatgctgg aagttctcgt cattcatgat gcggatctgc agatcaacgt tggttttgat
     5641 accgtcgatg atcagctcct gcagcgcatt cttcatgcgg gcaatcgcca cgtcacggtt
     5701 ttcaccgtag caaatcagct taccgatcat tgagtcatag tacggcggta cggtgtagcc
     5761 cgcgtagata tgagactccc aacgtacgcc aaaaccgcca ggtgcgtgga aacgggtgat
     5821 tttgcctggg cttggcagga aggtgttcgg atcttcggcg ttgatacgac attccaccgc
     5881 atggccgcga acgtgaactt cttcttgctt gatcgacagc ggttgaccgg cagcgatacg
     5941 cagctgttct ttgatcaggt caacgccggt gatcatttct gtaaccgggt gttctacctg
     6001 aatacgggtg ttcatttcga tgaaatagaa ctcgccgttt tcgaacagga actcgaaagt
     6061 acctgcaccg cgatagccga tatcaacaca cgctttagcg caacgttcgc cgatgtagcg
     6121 acgcagttcc ggggtaatgc ccggtgctgg tgcttcttcg accactttct ggtggcggcg
     6181 ctgcatggag cagtcacgtt ccgccagata gatagtgttg ccctgaccgt cagccagtac
     6241 ctgaatctcg acgtggcgag gattctccag gtatttctcc atgtaaacca tatcgttgct
     6301 gaaagcagct ttcgcttccg cacgggtcat ggagatggat tgtgccagtt cagcgtcgcc
     6361 gcgcactacg cgcataccgc gaccgccgcc gccgccggag gctttgataa tcaccggata
     6421 accaatgcgt ttagcaatgg cgcggttttt atccatatcg tcgcccagcg ggccgtcaga
     6481 accaggtacg caaggaacgc ccgctttttt catcgccgcg attgcggata ctttgtcgcc
     6541 catcaggcga atggtttctg ctttcgggcc gatgaagata aagccggagc gttcaacctg
     6601 ctcggcaaag ttggcgttct cggagaggaa gccgtaaccc ggatggattg ctactgcgcc
     6661 ggtgatttca gcggcgctga tgattgccgg gatgttcaga taactttgta ctgacggagc
     6721 agggccaata cagaccgttt catctgccag taatacgtgt tttagatcgc gatccgcgct
     6781 ggagtgcaca gcgacagtct tgatgcccag ttctttacag gcacgaagaa tacgcaatgc
     6841 aatctcgccg cggttggcaa taacaatttt atccagcatg ttcgcctcgt tactcgatga
     6901 cgaccagcgg ctcgtcaaat tctaccggtt gtccactttc gaccagaatt gctttcacgg
     6961 taccggattt gtccgcttcg atctggttca tcattttcat ggcttcaacg atgcacaggg
     7021 tatcgcccac gttgactttc tgacccactt cgatgaacgc ttttgcgtcc gggcttgggg
     7081 tgcggtagaa agtaccaacc atcggggaac gtacgatgtg accactgatt tccgctgctg
     7141 ctggcgcttc catggaagga acggtcgccg gagcggctgc gttagattga gctggctgct
     7201 gcatcattgg tgcagcgtaa gcttgttgca tcacagggaa acttgcggca ggagctgcac
     7261 ggctaatgcg tactgactct tcgccttcag aaatttccag ttcggagatg cctgattctt
     7321 caaccagctc gatcagtttt ttaatcttac gaatatccat gagtgggttc cgtactcttt
     7381 gtttagtgtg attgtgacag gcgtttcacc gccgtctgta aagcgtatgc cccgacagct
     7441 gtatgcatag cgataaattc cagcaggccg ggacgccggt ctattttgcc ccagagtgtc
     7501 aatgttagac ttgacggaca ttgtgcagcg atctgctgct atcctccggc aaaaaacaaa
     7561 atataccttc gttgcgccaa tgtcaccttt tccgcagcgc aaaaactgcg tcagggagga
     7621 cgaggtcgca cattataacg atttcgtagc aattggcagc taaatactgg tcttatcagg
     7681 gaagataatc aacagctaac atgtaaataa ccttcaacac cgtgtaattt gcaacaagcc
     7741 gcacaattca cgaaattagc gccatcatcg acggaacttc ttataacgta aggtgaaaag
     7801 ctgaaaagcc agccctgcga agatgagggg ctgcaatgag ataatcttca cagaccacaa
     7861 ataatgtagg gatgccagga tcgcgacaag atagacgaca ttacgcagcg gttgctgggg
     7921 attacccaat tttcactgca tcgtctggat agatgtgaat gctaaagcac gcaaaatcaa
     7981 ccaactgaca attctcagca ttaaataggt tcgagtggtt aatttgtagt caaataaaat
     8041 cagattattc accccatggt accagaaatc agtaacaggt taagcgtagt gtcgcccagg
     8101 caaagcacca taaacctaac aggcgacgga ggcgtatcaa taatggctgt ttagaggagt
     8161 atcaatgcgc atcaatttgt ggtcagtcga aatcatgact ttccagtttg gatgatgttg
     8221 tacaacttcc tgaccaaggt gttcgacgaa agctgtccgg ctaaccgctt ttcccgttaa
     8281 ccgattgaat aatgatgtca aaatgagttc cctctcttat tattccttgc taaatggtta
     8341 gttaaccttc accagcgtgc gaccctggat ctggttatta atgatggcct cggcaaagtt
     8401 cggtgcctct gacagagata tctcttttgc cgcctgggta tagaatgatt ccggtaaatc
     8461 ggcgaccagt cgctgccagg cttgtgcgcg gcgttctggt ggcgtcatta ctgaatccac
     8521 cccttgcaaa cggacattac gcagaataaa tggcatgacc gtgcttggca gagtaaaacc
     8581 acccgccaga ccacaggccg ccacgcagcc gccgtaattc atttgcgcca gcacttttgc
     8641 cagcactttg tcgccaacag tgtcaattgc cccagcccag acttgttttt ccagaggacg
     8701 ggattcggca aactcatcac gagggagaat acggctggca cctaaacttt tcagatattc
     8761 atgggtactt tcgcgaccgg aaacggcaac gacctgataa cccaacttat gcagcagcgc
     8821 cacggcggta ctgccgacgc caccactggc acccgtcacg acaatctccc cgtcctgcgg
     8881 gcgaacaccg gcatcttcca gcgccatcac acacagcatg gcggtaaaac cggcagtacc
     8941 gataatcatt gctttacgcg cgtccagccc ttgcggcatg gcaaccagcc agtcaccttt
     9001 cactcgcgcc tgctccgcca gcccacccca gtggttttca ccaacgcccc agccagtgag
     9061 taacacctcc tgaccggcat gaaaacgcgg atcttcgctg gtgcgtacag ttccggcaaa
     9121 atcgatccca ggaatcatcg gaaaattacg gatgattttt cccttaccgg taatcgccag
     9181 cgcatcttta tagttcaggc tcgaccagtg aacatcgacc gtgacatcgc cctccggcag
     9241 gcgactttcg tccagagtct gtactgatgc gagagttttg ccgtcctgct gttctaaaag
     9301 taacgcctgc ataagtggtc ctcatgtgca tgatggattg gaaaataatt tctgaagact
     9361 atactcgcta atggaaaagc aaagccgatg aagcgcaata aattgcgaga taaatctgat
     9421 ttgctagtat gcccgcttcc tcactatcgg agttaacaca aggatgagat taacgacgaa
     9481 attttcggcc tttgttacgc tgctcaccgg gttaacaatt tttgtgactt tgctgggctg
     9541 ttcgctaagt ttctacaacg ccattcagta taagtttagt catcgcgttc aggcggtggc
     9601 gacggcgatt gatacccacc ttgtgtcgaa tgacttcagc gcattaaggc cacaaattac
     9661 cgaattaatg gtgtcagcag atatcgttcg tgtagacctg ctgcatggtg ataaacaggt
     9721 ttataccctg gccagaaatg gtagttatcg tccggttggc accagcgatc tgtttcgtga
     9781 actgagcgtt ccgttgataa agcatccggg gatgtcgttg cgtctggttt atcaggatcc
     9841 gatgggcaac tatttccatt cgttgatgac caccgcgccg ctcacggggg cgattggctt
     9901 tatcattctt atgctctttc tggcggtacg ctggttacaa cggcaacttg ccgggcaaga
     9961 attgctggaa acccgggcta ctcgtatctt aaacggtgag cgtggctcta atgtgttggg
    10021 aaccatctat gaatggccgc ccagaaccag cagtgcgctg gatacgctgc ttcgtgaaat
    10081 tcagaacgca cgcgaacaac acagccgtct tgatacgctg atccgctctt atgccgcaca
    10141 ggacgtgaaa accggcctca ataaccgact ctttttcgat aatcagttag caacgttact
    10201 ggaagatcag gagaaagtag gtacccacgg gatcgtgatg atgattcgtc tgccggattt
    10261 caatatgttg agcgatacct gggggcacag ccaggttgaa gaacagttct tcactctgac
    10321 gaatctgctg tcgacattta tgatgcgcta ccctggcgca ctgctggcgc gttaccaccg
    10381 cagtgatttt gctgcgctgt taccgcaccg gacgttaaaa gaggcagaga gcatagccag
    10441 tcagttaatc aaagccgtcg ataccttgcc gaacaataaa atgctcgatc gcgacgatat
    10501 gatccacatt ggtatctgtg cctggcgtag tggtcaggat accgagcagg taatagaaca
    10561 tgcagagtct gccacgcgta atgcgggatt gcagggtggc aatagctggg ctatttatga
    10621 tgactcgttg cctgaaaaag gacgcggtaa tgttcgctgg cgtacgctta tcgagcaaat
    10681 gctcagtcgc ggcggcccgc gcctttatca aaaaccggcg gttactcgcg aaggtcaggt
    10741 tcatcatcgc gaactcatgt gccgcatctt cgatggtaat gaagaggtta gctcggcgga
    10801 gtatatgccg atggtcttgc agtttggctt atcggaagag tatgaccgtc tgcaaatcag
    10861 ccgtcttatt ccactattgc gttactggcc agaggaaaat ctggcgattc aggttaccgt
    10921 tgagtcgctg attcgtccgc gttttcagcg ttggctgcgt gatacgttaa tgcaatgtga
    10981 aaaatcacaa cgaaaacgca taattattga acttgcagag gccgatgtag gtcaacatat
    11041 cagtcgttta caacctgtta ttcgtttagt gaatgcttta ggggtacggg tagccgtcaa
    11101 ccaggctggt ttgacgctgg taagcaccag ttggatcaaa gaacttaatg ttgagttact
    11161 caagctccat ccggggctgg tcaggaacat tgagaagcga acggagaacc agctgctggt
    11221 tcaaagcctg gtggaagcct gctccgggac cagcacccac gtttacgcca ccggcgtgcg
    11281 ttcgcgaagc gagtggcaga ccctgattca gcgcggtgtt acaggcgggc aaggggattt
    11341 tttcgcgtcc tcacagccac ttgatactaa cgtgaaaaaa tattcacaaa gatactcggt
    11401 ttaacctgcc gtttaatccg ttttcacgta gaataacgcg cgctgcgtct catgggagtg
    11461 tgcttgtctg ctcgccagat tgttgcagca catatgcaga tgaatgacct tacgcggttg
    11521 caaacaggcg aggaatgctg ctgatgcatt aagcctttct ggactcaggc agagatttgt
    11581 aacaaaggaa acgaactgca ctaattttca ccgtagcaga tgatttttgc gccttgtcgc
    11641 tgctgcgtgt ggttggtaaa gtaagcggat tttcttttcc gccccagctt tcaggattat
    11701 cccttagtat gttgaaaaaa tttcgtggca tgttttccaa tgacttgtcc attgacctgg
    11761 gtactgcgaa taccctcatt tatgtaaaag gacaaggcat cgtattgaat gagccttccg
    11821 tggtggccat tcgtcaggat cgtgccggtt caccgaaaag cgtagctgca gtaggtcatg
    11881 acgcgaagca gatgctgggc cgtacgccgg gcaatattgc tgccattcgc ccaatgaaag
    11941 acggcgttat cgccgacttc ttcgtgactg aaaaaatgct ccagcacttc atcaaacaag
    12001 tgcacagcaa cagctttatg cgtccaagcc cgcgcgttct ggtttgtgta ccggttggcg
    12061 cgacccaggt tgaacgccgc gcaattcgtg aatccgcgca gggcgctggt gcccgtgaag
    12121 tcttcctgat tgaagaaccg atggctgccg caattggtgc tggcctgccg gtttctgaag
    12181 cgaccggttc tatggtggtt gatatcggtg gtggtaccac tgaagttgct gttatctcct
    12241 tgaacggcgt ggtttactcc tcttccgtgc gcattggtgg tgatcgtttc gacgaagcta
    12301 tcatcaacta tgtgcgtcgt aattacggtt ctctgatcgg tgaagccacc gcagaacgta
    12361 tcaagcacga aatcggttcg gcttacccgg gcgatgaagt ccgtgaaatc gaagttcgtg
    12421 gccgtaacct ggcagaaggt gttccacgcg gttttaccct gaactccaac gaaatcctcg
    12481 aagcactgca ggagccgctg accggtattg tgagcgcggt aatggttgca ctggaacagt
    12541 gcccgccgga actggcttcc gacatctccg agcgcggcat ggtgctcacc ggtggtggcg
    12601 cactgctgcg taaccttgac cgtttgttaa tggaagaaac cggcattcca gtcgttgttg
    12661 ctgaagaccc gctgacctgt gtggcgcgcg gtggcggcaa agcgctggaa atgatcgaca
    12721 tgcacggcgg cgacctgttc agcgaagagt aatcggatgc aggcagggga agcgtctgtt
    12781 taccctgcct ggtctgatac gagaatacgc ataacttatg aagccaattt ttagccgtgg
    12841 cccgtcgcta cagattcgcc ttattctggc ggtgctggtg gcgctcggca ttattattgc
    12901 cgacagccgc ctggggacgt tcagtcaaat ccgtacttat atggataccg ccgtcagtcc
    12961 tttctacttt gtttccaatg ctcctcgtga attgctggat ggcatatcgc agacgctggc
    13021 ctcgcgtgac caactagaac ttgaaaaccg ggcgttacgt caggaactgt tgctgaaaaa
    13081 cagtgaactg ctgatgcttg gacaatacaa acaggagaac gcgcgtctgc gcgagctgct
    13141 gggttccccg ctgcgtcagg atgagcagaa aatggtgact caggttatct ccacggttaa
    13201 cgatccttat agcgatcaag ttgttatcga taaaggtagc gttaatggcg tttatgaagg
    13261 ccagccggtc atcagcgaca aaggtgttgt tggtcaggtg gtggccgtcg ctaaactgac
    13321 cagtcgcgtg ctgctgattt gtgatgcgac ccacgcgctg ccaatccagg tgctgcgcaa
    13381 cgatatccgc gtaattgcag ccggtaacgg ttgtacggat gatttgcagc ttgagcatct
    13441 gccagcgaat acggatattc gtgttggtga tgtgctggtg acctccggtc tgggcggtcg
    13501 tttcccggaa ggctatccgg tcgcggttgt ctcttccgta aaactcgata cccagcgcgc
    13561 ttatactgtg atccaggcgc gtccgactgc aggactgcaa cgtttgcgtt atctgctgct
    13621 gctgtggggc gcggatcgta acggcgctaa cccgatgacg ccggaagagg tgcatcgtgt
    13681 tgctaatgaa cgtctgatgc agatgatgcc gcaggtattg ccttcgccag acgcgatggg
    13741 gccaaagtta cctgaaccgg caacggggat cgctcagccg actccgcagc aaccggcgac
    13801 aggaaatgca gctactgcgc ctgttgcgcc gacacagccc gctgctaatc gctctccaca
    13861 aagggctacg ccgccgcaaa gtggtgctca accgcctgcg cgtgcgccgg gagggcaata
    13921 gtggcgagct atcgtagcca gggacgctgg gtaatctggc tctctttcct cattgcgctg
    13981 ttgctgcaaa tcatgccctg gccggataac ctgattgttt tccggccaaa ctgggtgtta
    14041 cttatcttgt tgtattggat cctggccttg ccccatcgcg taaatgtggg cacaggtttt
    14101 gtgatgggtg ccatactgga tctgatcagc ggctcgatgc ttggcgtacg cgtattggcg
    14161 atgagcatca ttgcttacct ggtggcgctg aaataccagc ttttccgcaa cctcgcatta
    14221 tggcagcagg cgctggtcgt catgttgctt tcgctggtgg tggatattat tgttttctgg
    14281 gcagagtttt tagtgattaa cgtctctttc agaccggaag tgttctggag tagtgtagtc
    14341 aatggggtgc tctggccgtg gattttcttg ctgatgcgta aagtccgtca gcagtttgcc
    14401 gtgcaataaa ggtttctatg acttctctgt atttagcttc cggttctccg cgtcgtcagg
    14461 agttacttgc gcaacttggc gtgacctttg aacgtattgt tacgggcatt gaggagcagc
    14521 gtcagccgca ggagagcgcg cagcagtatg ttgtgcgtct ggcacgcgag aaagcacagg
    14581 cgggtgtcgc gcaaacggca caggatctcc cggtgctggg tgcggatatt atcgttatcc
    14641 tgaacggaga agtgctcgag aaaccgcgcg acgcagagca tgcggcgcag atgttgcgca
    14701 aattatcggg tcagacccat caggtgatga cggcagtggc gttggccgac agccagcata
    14761 ttctcgattg cctggtggtc accgatgtga ctttcagaac gttaacagac gaagacatcg
    14821 cgggctatgt cgccagcggt gaaccgttag ataaagcagg tgcatacggt attcaggggc
    14881 tgggtggctg ttttgtcagg aagataaatg gcagctatca cgccgtagtc ggcttaccgc
    14941 tggttgaaac gtatgaatta ttaagtaatt ttaacgcact gcgtgagaaa agggataaac
    15001 atgacggctg aattgttagt aaacgtaacg ccttcggaaa cgcgagtggc gtatattgat
    15061 ggcggtattc tgcaggaaat tcatattgaa cgtgaggcgc gacgcggaat agtaggcaat
    15121 atctacaagg gtcgtgtaag tcgtgtactt ccgggtatgc aggcggcttt tgtagatatt
    15181 gggctggata aagccgcgtt tcttcatgca tccgacatca tgccgcatac cgaatgtgtg
    15241 gcgggtgaag aacaaaagca attcacggtg cgcgacatct cggaactggt tcgtcagggg
    15301 caagatctga tggtgcaggt ggtgaaagat ccgcttggca ctaaaggtgc gcgcctgacc
    15361 accgatatca cgctgccttc tcgctatctg gtatttatgc caggggcttc tcacgttggg
    15421 gtttcccaac gaattgaaag cgaatcagaa cgtgaacgcc tgaaaaaagt ggtcgcagag
    15481 tattgcgacg agcagggcgg gtttatcatc cgcaccgcag cggaaggggt tggcgaggct
    15541 gaactggcct ccgatgccgc ttatctgaaa cgcgtctgga ccaaagttat ggagcgtaaa
    15601 aagcgcccgc agacccgtta tcagctgtac ggcgaactgg cgctggcgca gcgtgttctg
    15661 cgtgattttg ccgacgccga actggaccgc attcgcgttg actcacgcct gacttacgaa
    15721 gcattgctgg agtttacctc ggagtacatt cccgagatga ccagcaagct ggagcattac
    15781 accggacgcc agccgatttt cgatctcttt gatgtcgaaa acgaaatcca gcgagcgttg
    15841 gaacgcaaag tagaactgaa atccggtggt tatctgatta tcgaccagac cgaagcgatg
    15901 accaccgtgg acatcaatac cggagcgttt gtcggtcatc gcaatctgga cgacactatt
    15961 ttcaatacca atattgaagc gacgcaggct atcgctcgcc agttacggtt gcgtaatctg
    16021 ggcgggatta tcattattga tttcatcgat atgaataatg aagatcaccg ccgccgcgtg
    16081 ctgcattcgc tggagcaggc gttgagcaaa gaccgggtga aaaccagcgt taatggtttt
    16141 tcggcgctgg ggctggtgga gatgacgcgt aaacgcaccc gcgaaagcat tgagcacgta
    16201 ctgtgtaacg aatgcccaac ctgccacggt cgcggaacgg tgaaaaccgt ggaaacggta
    16261 tgctatgaaa tcatgcgcga gattgttcgt gtccaccatg cttacgactc cgaccgtttc
    16321 ctggtctatg cttctccggc agtagccgaa gccttgaaag gcgaagagtc acactcgctg
    16381 gcggaagtgg aaattttcgt tggcaaacag gttaaagtac aaattgaacc gctctataac
    16441 caggagcagt ttgacgtcgt aatgatgtaa acagatgctg ggccgccatc cggcaaaggg
    16501 tttttgagtc acatttttag cagacaagga gtgacgggtg aggcgattgc cggggatttt
    16561 actgcttact ggagccgcgc tcgttgtgat cgctgccctg ctggttagcg gcctgcgtat
    16621 tgctttaccg catcttgacg cctggcgtcc ggaaatcctc aacaaaatag aatccgcgac
    16681 tggcatgccg gtagaagcca gtcagctctc agccagctgg cagaattttg gcccgacgct
    16741 tgaagcacac gacatccgtg cagaactaaa agatggcggc gaattttcgg ttaaacgcgt
    16801 tactctggcg ctggatgtct ggcagagcct gttacatatg cgctggcagt ttcgcgacct
    16861 cactttctgg cagctgcgct ttcgcaccaa cactcctatc accagcggtg gtagcgatga
    16921 cagtctggaa gccagtcaca tcagcgatct gtttcttcgt caatttgacc atttcgatct
    16981 ccgcgacagt gaagtcagtt ttctgacgcc atccggtcag agcgccgagc tggcgatccc
    17041 acaactcacc tggctgaacg atccacgtcg acaccgtgcg gaaggcctgg taagcctctc
    17101 cagccttacc gggcagcacg gcgtgatgca ggtgcgcatg gatttgcgcg atgatgaggg
    17161 gttgttaagc aatggtcgcg tctggctaca ggcggatgac atcgacctga agccgtggct
    17221 cggtaaatgg atgcaggaca atattgctct ggaaacggca cagttctccc ttgaaggctg
    17281 gatgacgatc gacaaaggcg atgtaaccgg cggtgacgtc tggctgaaac agggcggtgc
    17341 cagctggttg ggcgagaagc aaacgcatac gctgtcggtg gataatctga ccgcgcatat
    17401 tacgcgtgaa aatccgggct ggcagttctc tattcccgat acacggatca cgatggacgg
    17461 caaaccctgg ccgagcggag cattgacgct ggcctggata ccggaacagg acgttggcgg
    17521 caaagacaat aaacgcagtg acgaactccg gattcgcgct agtaatctgg agctggcagg
    17581 cctggagggc atacgcccgc tggccgcgaa actttcacct gcactgggtg atgtttggcg
    17641 ctccacacaa ccgagcggca agattaacac tctggcgctg gatatcccgc ttcaggcggc
    17701 agacaagacc cgttttcagg catcgtggag cgatctggcc tggaagcaat ggaaattatt
    17761 accgggtgcg gaacacttct ccgggacgct ttccggcagc gttgaaaatg gtttgcttac
    17821 cgcgtcgatg aagcaggcaa agatgcctta cgaaacggta ttccgtgcgc cactggaaat
    17881 cgccgacggc caggcaacta taagctggct gaacaatgac aaaggtttcc agctggatgg
    17941 gcgtaatatt gacgttaaag ccaaagccgt ccatgcgcgc ggcggttttc gttacctgca
    18001 acctgctaac gatgaaccct ggctgggtat tctggctggc atcagtaccg atgatggttc
    18061 acaagcctgg cgctatttcc cggaaaactt gatgggtaaa gacctggttg attatttaag
    18121 tggtgcgatt cagggcggtg aagcggataa cgcgacgctg gtttatggtg gcaatccgca
    18181 actcttcccc tataaacaca acgaaggtca gtttgaagtg ctggtgccgc tgcgcaacgc
    18241 gaagtttgcc ttccagccgg actggcctgc attaactaac cttgatattg aactggactt
    18301 tattaacgac ggtttatgga tgaaaaccga tggcgttaat ctgggcggcg tgcgcgcgag
    18361 taatctcacc gcagtgatcc ctgactactc aaaagaaaaa ctgctgattg acgctgacat
    18421 taaaggtccg ggtaaagccg ttggccctta ctttgatgag acaccgctga aagattctct
    18481 gggtgcgacc ctgcaagaac tccagctcga cggcgatgtg aatgctcgct tacatcttga
    18541 tatcccgctg aacggcgaac tggtaaccgc gaaaggtgaa gtgacgctgc gtaataacag
    18601 tctgtttatc aaaccactcg acagcatcct gaaaaatttg agcggtaaat tcagctttat
    18661 caatggcgat ctgcaaagtg aaccactgac agcaagctgg tttaatcagc cgttgaacgt
    18721 ggatttttcc accaaagaag gggcaaaagc ctaccaggtt gcggtgaatc tcaacggtaa
    18781 ctggcaaccg gcgaaaaccg gcgttctgcc tgaagcggtg aacgaagcat tgagtggcag
    18841 cgtggcgtgg gatggtaaag tgggcattga tctgccttat catgctggcg cgacctataa
    18901 cgtagagctg aacggcgatc tgaataatgt gagtagtcac ttaccttcac cgttagccaa
    18961 acctgcgggt gaaccactag cggtaaacgt taaggttgat ggcaatctca acagctttga
    19021 attaaccgga caggctggtg cggataacca tttcaatagc cgctggttgc ttggtcaaaa
    19081 gctgacgctc gatcgtgcta tttgggcggc agacagtaaa acgctcccgc cgttgccgga
    19141 acaaagtggc gttgaactta atatgccgcc gatgaatggt gccgagtggc tggccctgtt
    19201 ccagaaaggc gctgcggaga gtgtcggtgg tgcagcgagt ttcccacaac acataacatt
    19261 acgtacgcct atgttgtcac tgggaaatca gcaatggaat aacctgagta ttgtttcgca
    19321 accgacggca aatggcaccc tggttgaggc gcaagggcgt gaaatcaacg ccacgctagc
    19381 gatgcgtaat aacgcgccgt ggctggcgaa tatcaaatat ctttattaca acccgagcgt
    19441 ggcgaaagct cgtggtgatt caacgccgtc atcacctttc ccgacaacgg agcgcattaa
    19501 cttccgtggc tggccggacg cacaaatacg atgcacagag tgctggttct gggggcaaaa
    19561 attcggtcgc attgacagtg atatcaccat ttctggcgat acgttaacgc tgaccaatgg
    19621 actgattgat actggtttct cgcggcttac tgccgatggt gaatgggtta ataatccggg
    19681 gaatgaacgt acctcgctga aaggaaaact gcgtgggcag aaaattgatg ccgccgcaga
    19741 attttttggt gtcacgacgc ccatacgcca gtcgtcattt aatgtggatt acgatttaca
    19801 ctggcgcaaa gcaccctggc agccagatga agcgacgttg aatggcatca ttcatactca
    19861 actgggtaaa ggcgaaatta ccgaaatcaa taccggacat gccgggcaat tgctgcgctt
    19921 attgagcgta gatgccctga tgcgtaagct gcgttttgat ttcagagaca cttttggcga
    19981 agggttctat tttgactcca ttcgcagcac cgcgtggatt aaagacggcg ttatgcacac
    20041 cgacgacacg ctggtggatg gcctggaggc ggatatcgcc atgaaagggt cggtaaatct
    20101 ggtacgtcga gacctgaata tggaagcggt tgtcgcacca gagatttctg cgacggtggg
    20161 cgtggctgcg gcttttgcgg ttaaccccat tgttggcgcg gcagtgtttg ccgccagtaa
    20221 agtgctgggg ccgctgtgga gcaaagtctc cattttgcgc tatcacattt cgggtccgct
    20281 ggatgatccg caaatcaacg aagtgttgcg ccaaccgcgt aaagaaaaag cgcaatgatt
    20341 tgacgagggc gcctaattgc cccaatctca tatgataatc gttgccaaag gccaacgagc
    20401 cggaacataa ctgtaggccg gatagggcgt tcacgccgca tccggcagcc gtaaaaaatc
    20461 ctctactgca gtaactaacg agtagcaaaa acgatgagtc tgaacctggt aagtgaacaa
    20521 ttgctagcgg cgaacggcct gaaacatcag gacttgttcg cgatcctcgg tcaactggcc
    20581 gaacgtcgcc ttgattatgg cgatctctat tttcagtcga gctatcacga atcctgggtt
    20641 ttagaagacc gcattattaa agatggttct tacaacatcg atcagggcgt tggcgtgcgt
    20701 gcaatcagcg gtgaaaaaac cggatttgct tacgctgacc aaatcagcct gctggcgctg
    20761 gaacagagtg cgcaagcggc gcgcaccatc gtccgtgata gtggtgatgg caaagtacag
    20821 acgctgggcg cggtagagca tagcccgttg tatacctcgg tagatccgct gcaaagcatg
    20881 agccgtgaag agaagctgga tatcctgcgt cgcgtcgata aggttgctcg cgaagcggac
    20941 aagcgcgtac aggaagtgac tgccagcctc agtggtgtct atgaattaat tctggttgcg
    21001 gccaccgacg gcacgctagc ggcggatgtc cgtccgctgg tgcgtctttc cgtgagcgtt
    21061 ctggtcgaag aagatggcaa acgcgaacgc ggtgccagtg gcggcggcgg tcgttttggt
    21121 tatgagttct tccttgccga tcttgacggc gaagtccgtg cggatgcatg ggcaaaagaa
    21181 gcagtgcgta tggcgctggt caatctttct gccgttgctg caccagcggg caccatgccg
    21241 gtagtacttg gcgcaggttg gccgggcgtg ctgttgcatg aagcggtagg tcacggtctg
    21301 gaaggcgact tcaaccgccg tggcacttca gtatttagtg gacaggtcgg ggagctggtg
    21361 gcttcagaac tgtgtaccgt ggttgatgac ggcacgatgg tcgatcgccg tggttcggtg
    21421 gcgattgatg acgaaggtac gccaggccag tacaacgtgc tgattgagaa cggcattctg
    21481 aaaggctaca tgcaggataa actcaacgcg cgtttgatgg ggatgacgcc gactggcaac
    21541 ggtcgccgtg aatcctacgc ccatctgccc atgccgcgta tgaccaacac ctatatgctg
    21601 ccgggtaaat cgaccccgca agaaattatt gaatccgttg agtacggtat ctatgcaccg
    21661 aactttggtg gcggtcaggt ggatatcacc tccggcaaat tcgttttctc cacttcagaa
    21721 gcatatctga ttgaaaacgg taaagtaacg aagccggtga aaggcgcaac gttgattggt
    21781 tccggtatcg aaaccatgca gcagatttcg atggttggca acgacctgaa actggataac
    21841 ggcgtgggtg tctgcggtaa agaagggcaa agtttgccgg ttggcgtggg ccagccaacg
    21901 ttgaaagtcg ataacctgac tgttggcggt actgcgtaat aatttcatta ttttcagaag
    21961 gataattaat ctttctacgt gcacgaacgg tcccctcgct cctctggggt tagggtgagg
    22021 ggaacccgtt ggcacaggtt tgtagaacgt aacagtacaa tatgaattac ttctctttcc
    22081 cgcgcccgtg catctcctga aacaatttac cgacctcaac aaaataatcc gtcagcgagt
    22141 tgatcacgac ctgtaccttc agcggcagct tatctttttc ggtatataac gcataaaccg
    22201 ggcgtggatc tgactgataa cgcggcagca ggatctccag ctccccacga ttgatctcgt
    22261 tgatcaccca catcagcggc acgtaggcga tcccggcacc cgccgttagc cagcgcacca
    22321 gcgtcatcgg atcattagtc acaaatctcc cttgcgggat caggcgagtc gagatccctt
    22381 ccggtgcaat cagttcaaat tcattgtcgg gccgcacgct gtattcaagc catgaatggc
    22441 tacttaaatc ggcgggtttt tccggtatgc cgtattgtgt gagatagctt ttcgcggcgc
    22501 acaccaccat tggcatcgcg ccaagacggc gggaaaacag gctggaatcc tgcaacgcgc
    22561 cgacgcggat caccacatcc agaccgtcgg caatcaggtc gggggctgga attccggtaa
    22621 ccagattggc gctcaaacct gggtattctt tcagcatttt ggctgtcagc ccggcgagaa
    22681 cattttgtgc catagttgaa gaacagccaa tgcgtagcgt cccgatgggg gtgttattga
    22741 aggcatacag ttgctcatga acatcctgca cttcatgaag catacgacgg cagccctggt
    22801 agtaaattct accggcttcg gtcaggccaa tgctgcgtgt gctacggttt aacagcttta
    22861 cctgcaactc atcttccagt tttgacaccg tctgactgat ggacgaaacg ctcatctgta
    22921 gctgtctggc ggcggcggta aaagagccaa attcaactac tttggcaaac accgacatgc
    22981 gttttagtcg ttccattatt cactctgact taaaagtgat ttagatcaca taatatagat
    23041 aacagcataa cagttacgct aatatattaa atatcaatct acagcaatgt tgctctcgcc
    23101 cggctttcca tgcccttctc tgcggcgaca gatgctgaaa ataataacgc ctgctctctc
    23161 tatacaacca aggtcaacat gagtctgttt cccgttatcg tggtgtttgg gctgtccttc
    23221 ccaccgatat tttttgaatt gcttttatca ctggcgattt tctggctggt gcgccgggta
    23281 cttgtgccaa caggtatcta cgactttgtc tggcatccgg cgttgttcaa caccgcgctc
    23341 tattgctgct tgttttattt gatatcgcga ctgttcgttt gaggttgaag tgaaaacact
    23401 aataagaaaa ttctcccgta cggccatcac ggtcgtatta gtcattctgg ccttcatcgc
    23461 aatttttaat gcctgggtct attacaccga atccccctgg acgcgtgacg cgcgctttag
    23521 cgctgacgtc gttgcgatcg cgccggacgt ttctggactc attacccagg tgaatgttca
    23581 tgataaccag ctggtgaaaa aaggacagat actgttcacc atcgaccagc cgcgctatca
    23641 aaaggcgctt gaggaagcgc aagccgatgt tgcttattat caggtactgg cacaggagaa
    23701 acgccaggag gccggacgtc gtaaccgtct cggtgtgcag gcgatgtctc gcgaagagat
    23761 cgaccaggct aacaacgtac tacaaacggt tctgcatcag ttagcgaaag cgcaggcgac
    23821 ccgcgatctg gcaaaactgg atcttgaacg cacggtgatc cgagcgccag cagatggctg
    23881 ggtgaccaac ctcaacgtct ataccggtga gtttattact cgaggatcaa cggcggttgc
    23941 gctggtgaaa cagaactcct tctatgtact ggcctatatg gaagaaacta agctggaagg
    24001 ggtgcgtccg gggtatcgtg cagagatcac gccgcttggc agtaacaaag tgctgaaagg
    24061 gactgttgat agtgttgccg ctggggtcac caacgccagc agcacgcgtg acgacaaagg
    24121 gatggcgacc atcgactcta accttgaatg ggtgcgtttg gcgcaacgtg ttccggttcg
    24181 tattcgtctc gacaaccagc aagagaacat ctggcctgcg ggcaccactg ctacagtggt
    24241 ggtcactggc aaacaagatc gcgacgaaag ccaggattcg ttcttccgta aaatggccca
    24301 tcgcctgcgt gagtttggtt aatcacgatg ggtattttct ccattgctaa ccaacatatt
    24361 cgctttgcgg taaaactggc gaccgccatt gtactggcgc tgtttgttgg ctttcacttc
    24421 cagctggaaa cgccacgctg ggcggtactg acagcggcga ttgttgccgc cggtccggcc
    24481 tttgctgcgg gaggtgaacc gtattctggc gctattcgct atcgtggctt tttgcgcatc
    24541 atcggcacat ttattggctg tattgccgga ctggtgatca tcattgcgat gatccgcgca
    24601 ccattattga tgattctggt gtgctgtatc tgggccggtt tttgtacctg gatatcctcg
    24661 ctggtacgaa tagaaaactc gtacgcgtgg gggctggccg gttataccgc gctgatcatt
    24721 gtgatcacca ttcagccgga accattgctt acgccgcagt ttgccgtcga acgttgtagc
    24781 gagatcgtta tcggtattgt gtgtgcgatt atggcggatt tgctcttttc tccgcgatcg
    24841 atcaaacaag aagtagatcg agagctggaa agtttgctgg tcgcgcaata tcaattaatg
    24901 caactctgta tcaagcatgg cgatggtgaa gttgtcgata aagcctgggg cgacctggtg
    24961 cgacgcacca cggcactaca aggcatgcgc agcaacctga atatggaatc ttcccgctgg
    25021 gcgcgggcca atcgacgttt aaaagcgatc aatacgctat cgctgacgct gattacccaa
    25081 tcctgcgaaa cttatcttat tcagaatacg cgcccggaat tgatcactga tactttccgc
    25141 gaattttttg acacgccggt agaaaccgcg caggacgtcc acaagcagct caaacgcctg
    25201 cggagagtta tcgcctggac cggggaacgg gaaacgcctg tcaccattta tagctgggtc
    25261 gcggcggcaa cgcgttatca gcttctcaag cgcggcgtta tcagtaacac aaaaatcaac
    25321 gccaccgaag aagagatcct gcaaggcgaa ccggaagtaa aagtagagtc agccgaacgt
    25381 catcatgcaa tggttaactt ctggcgaacc acactttcct gcattctggg cacgcttttc
    25441 tggctgtgga cgggctggac ttccggcagt ggtgcaatgg tgatgattgc ggtcgtgacg
    25501 tcactggcaa tgcgtttgcc gaatccacgc atggtggcga tcgactttat ctatgggacg
    25561 ctggccgcgc tgccgttagg gatgctctac tttttggtga ttatccctaa tacccaacag
    25621 agcatgttgc tgctgtgtat tagcctggca gtgctgggat tcttcctcgg tatagaagta
    25681 cagaaacggc gactgggctc gatgggggca ctggccagca ccataaatat tatcgtgctg
    25741 gataacccga tgactttcca tttcagtcag tttctcgaca gcgcattagg gcaaatcgtc
    25801 ggctgtgtgc tcgcgttcac cgttattttg ctggtgcggg ataaatcgcg cgacaggact
    25861 ggacgtgtac tgcttaatca gtttgtttct gccgctgttt ccgcgatgac taccaatgtg
    25921 gcacgtcgta aagagaacca cctcccggca ctttatcagc agctgttttt gctgatgaat
    25981 aagttcccag gggatttgcc gaaatttcgc ctggcgctga cgatgattat cgcgcaccag
    26041 cgcctgcgtg atgcgccgat cccggttaac gaggatttat cggcgtttca ccgacaaatg
    26101 cgccgcacag cagaccatgt gatatctgcc cgtagcgatg ataaacgtcg tcggtacttt
    26161 ggccagttgc tggaagaact ggaaatctac caggaaaagc tacgcatctg gcaagcgcca
    26221 ccgcaggtga cggaaccggt tcatcggctg gcggggatgc tccataagta tcaacatgcg
    26281 ttgaccgata gttaagtcaa aaccgacgcc aaaagcgtcg gttttttcat ggctatactt
    26341 agcgatgcac ggcagaactc gccgcgaaac gtgacggtgg caacagatga atatttatgc
    26401 ctttgatttt aatgagattg agagtcagga ggatttttat cgtgacttta gccaaacctt
    26461 tggtctggct aaagataagg tacgcgatct cgactcacta tgggatgtgt taatgaacga
    26521 tgtcctgccg ctaccacttg agattgaatt tgttcatctg ggagagaaaa cgcgtcgccg
    26581 ttttggcgcg ttaatattgc tgtttgatga ggcagaggaa gagctggaag ggcatttgcg
    26641 ttttaatgtt cgtcattagc gcaaaaaaaa agcccccgaa ccgggggcaa tatcgtcgga
    26701 caaaacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt caccgctacg
    26761 agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc
    26821 acgtatatcc attggcgaag acgccacacc acttacggat acggtcccga ttgcttcacg
    26881 attttgtgct tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag agagaacaga
    26941 aagtacgctt aatgcagcta cagtggtttt gattttcatg atttttacct cgacataatc
    27001 ttttagctgg gcctttgttt cgtgacccat atcacaaaat caagtacaca ctaattattg
    27061 aactaattaa taccacgcta actgtttcag ttgatactaa gtgttaaaaa tgtgaatttt
    27121 aataacaaaa aagtataaaa aacacggtta tttattatta attatagtat tttcaattag
    27181 ataatgaatt ttgcataatg tgcttttttt accgtatgta ttcattttgt gaatgatgtg
    27241 tcgcggtaaa acgcactatt cgttaaatgt atcgcaggag aaagaagggg aatgagaggg
    27301 ataagcaaca ttttccccgc cgccagaagc gacggggcag agattaaagc tcctggtcga
    27361 acagctctaa aatcgcttcg tacaggtctt tgacggtgaa accgttagca ggggtggtaa
    27421 agatggtgtc atcgccagcg atggtgccca gaataccttc tgctttgccc agtgagtcca
    27481 gcaggcgagc aattaactgc gccgcgccag ggctggtatg aatcacgaca actgcatcgt
    27541 tgtagtcgat atccagcacc agattcttca atggactgga ggtggttggt acacccagtt
    27601 cagctggcag gcagtaaacc atttccattt tggcattgcg tgtacgtaca gcaccaaact
    27661 tggtcaacat ccgcgagact ttagactgat taatattgtc aaagccttgc tcctgcaacg
    27721 cggcgacgat ttcgccctgg gagctaaatt tctcttcttt aagtaatgct ttaaatgctt
    27781 taactagttc ttcttgctta gccgagcttc gcataagtca cccgatatgg tggttgatac
    27841 aacattattg tgcatacaga tgaattttta tgcaaacagt cagccctgaa gaaggctgaa
    27901 ataatgttat gaaagagcgg gattttatca aatttcgtta ttgagaaaca tgcctgcgtc
    27961 acggcatgca aattctgttt aaaagtaaat taattgttat caaattgatg ttgttttggc
    28021 tgaacggtag ggtatattgt caccacctgt tggaatgttg cgctaatgca taagcgactg
    28081 ttaattacgt aagttaggtt cctgattacg gcaattaaat gcataaacgc taaacttgcg
    28141 tgactacaca ttcttgagat gtggtcattg taaacggcaa ttttgtggat taaggtcgcg
    28201 gcagcggagc aacatatctt agtttatcaa tataataagg agtttaggat gaaagtcgca
    28261 gtcctcggcg ctgctggcgg tattggccag gcgcttgcac tactgttaaa aacccaactg
    28321 ccttcaggtt cagaactctc tctgtatgat atcgctccag tgactcccgg tgtggccgtc
    28381 gatctgagcc atatccctac tgctgtgaaa atcaaaggtt tttctggtga agatgcgact
    28441 ccggcgctgg aaggcgcaga tgtcgttctt atctctgcag gcgtagcgcg taaaccgggt
    28501 atggatcgtt ccgacctgtt taacgttaac gccggcatcg tgaaaaacct ggtacagcaa
    28561 gttgcgaaaa actgcccgaa agcgtgcatt ggtattatca ctaacccggt taacaccaca
    28621 gttgcaattg ctgctgaagt gctgaaaaaa gccggtgttt atgacaaaaa caaactgttc
    28681 ggcgttacca cgctggatat cattcgttcc aacacctttg ttgcggaact gaaaggcaaa
    28741 cagccaggcg aagttgaagt gccggttatt ggcggtcact ctggtgttac cattctgccg
    28801 ctgctgtcac aggttcctgg cgttagtttt accgagcagg aagtggctga tctgaccaaa
    28861 cgtatccaga acgcgggtac tgaggtggtt gaagcgaaag ccggtggcgg gtctgcaacc
    28921 ctgtctatgg gccaggcagc tgcacgtttt ggtctgtctc tggttcgtgc actgcagggc
    28981 gaacaaggcg ttgtcgaatg tgcctacgtt gaaggcgacg gtcagtacgc ccgtttcttc
    29041 tctcaaccgc tgctgctggg taaaaacggc gtggaagagc gtaaatctat cggtaccctg
    29101 agcgcatttg aacagaacgc gctggaaggt atgctggata cgctcaagaa agatatcgcc
    29161 ctgggcgaag agttcgttaa taagtaattg attagcgaat aataaaaaac cggagcacag
    29221 actccggttt ttgttttgag cactcgactt aattggttgc cggatattcc tgaatggtga
    29281 cctgcagcgt taactgctta tcatcacgca tcactacaac cgggatcacc gaaccagggc
    29341 gaatttccgc cacctgatcc atcgtctcca gagcagagat ggccggtttg ttatccaccg
    29401 aaataatcag atcgttgacc tgaatacccg cattcgccgc tgggccgtca ggtgacactt
    29461 cattaaccac gatcccttgc agttgatcta taccaccgct ctgcgcgtgc agtggtgcga
    29521 tctcgcgtcc gccgatacca atgtagccgc ggatcacgcg accatcgcgg atcagcttat
    29581 ccataatttt ggttgctaac tggaaaggaa tcgcaaagcc gataccttcc ggcgtttcgc
    29641 catcgttact cttatcaaac gacagcgtgt taatgcccat cagttctccc agcgagttca
    29701 ccagcgcgcc gccagagtta ccgtggttaa tggaagcatc ggtttgtagg aagttttgcc
    29761 gcccggtcgg gttcagaccg attcgacccg tggcactaat aatcccttgg gtaatggtct
    29821 gcccgaggtt gtacgggtta ccgatcgcca gtactacgtc gccaatgtgc ggtacgcgac
    29881 gtgcattaat tggaatggta ggtaaaccgc cagtggcatt aattttaagt accgccagat
    29941 cggttagaga gtcagatccc accagcaatg cttcaaatac acgtccatcc tgtaaggcga
    30001 cgatgatctg atcggcgtcg ttgatgacgt gtttattggt gatgatataa ccgcgttgat
    30061 ccatgattac accggatccc agggtgcgga tctcaagctg gttgtgagag ttggtgttca
    30121 aaccacggtt gtaaacgtta accaccgctg gcgcggcgcg gcgaaccgcc agattatagc
    30181 tggcaggcgt ctcatcggta ctgtcaaatt gcggagtgga aagcgggtta aggctgcgca
    30241 gcgaaggcat ggcaaccagc agaatagcgc cgacaattaa tccaatcgca acggaacgta
    30301 agagcttcac aaacatgatg gaggcgtcat taaaaaaggg aacggcagca gcataccacg
    30361 agttaaccgg acatcacacg tcagcctgat gcccggttta cgacattaac gcatcagcag
    30421 atagatgctt tcattgccgc gtacaatttg cagggcgatg atggccggtt ttgccgccag
    30481 cactttacgc atttcagcaa tcgagttcac ccgatcgcgg ttgacgccaa tgatcacatc
    30541 gtctttttgc aagccagcct gagcagctgg gcttcctttg acaacttcat ctatcttaat
    30601 acctttgccg ccatctttta gctgaccatc gctcaacgtt gcaccttcca gcgctggcgt
    30661 gatcatttca gcgctggccg acgaagaggt gctggtatca agcgtcactt ctacttccag
    30721 tggtttgccg ttacgcagca ggccaagctt cactttcgtg cccggctctg tagtcgcgat
    30781 acgagagcgc aactcagcaa agctattcag cggtttgccg ttgaggctgg taataatatc
    30841 gcccgctttg acgcccgctt tcgctgagcc agaacttggc aacacttcgc tgacaaacgc
    30901 gccacgctgc acgtcaaggt tgaaggcttt ggcgatatcg gcactcatct cggtgccttt
    30961 gatgcctaac aaaccgcgtt tgatttcacc aaagtcgata agctgctgcg ccagtgttcg
    31021 cgccatatta ctggggatgg caaatccaat cccgacgctc ccgccgccag gcgcaaggat
    31081 tgcagtgttg atgccaatta actcaccgtt aaggtttaat agtgcaccgc cggagttacc
    31141 gcggttaatg gaagcatctg tctggataaa gttttccaga ccttcaagat tcaacccgct
    31201 gcggcctaat gcggaaacaa tgccagaggt ggcggtttgc ccaaggccaa atgggttacc
    31261 gaccgctacg gcaaaatcac cgacgcgcaa tttatcggag tcggcaatag cgatttgcgt
    31321 taatttgctc gggttttgaa tttgtaacag ggcgatatcg ctctggtcat cgctaccaat
    31381 cagttttgca tcaaactcgc gcccatcatt gagctgaata ctgattttct gtgcctgatt
    31441 aatcacatgg ttgttggtca gcacatagcc tttactggcg ttgatgatga caccggaacc
    31501 taaaccttcg aagggttgtg caggttgatc cggtaaatca tcaccaaaaa actttttgaa
    31561 ttcttccggg attttctgtc cctgactggc cgttccttcc acccgtacgc tcaccactgc
    31621 cggaagcact ttttccagca ttggagccag actggggaga ggggcctgat cggcaacctg
    31681 gcctggaatc gacgcgacgg cctgaaatga cgccgagaga gttaacccgg cacttaacgc
    31741 taatgcactc aacagctggg tttgtttttt cattattcct gctctcgtac ctgaatgata
    31801 agaaaagaga ttcaaaacag tttgatgtta ttgaattttc agacggctaa caatgagaca
    31861 ccagattaaa tgatagtcgg ggagggagag aagaggaggg cgcaatggct gcgcccgaaa
    31921 aataaattag tcgcgcttcg cgccagtacg cagcaggccg gatgcgcctt cagaatagtc
    31981 gcgaggcatc tgcaccggtg cctgatcgtt gctggcttca gactctgcca gacgattacg
    32041 gaacgggttt gcttcagcag acagttccgg cagcaggctg ctggagcttt ttgccatgtg
    32101 ctgatacagc tggcgatagt cgtgcgccat ggtatccagt aattccgcgc tgcgggcaaa
    32161 gtggctaacc agctcttcgc gatactcgtc cagttcagct ttattctttt ccagttcgta
    32221 ctgcaacgcc tgttgctggc gtagtttacg attaccaaaa cgcatggcca cagcaccaat
    32281 aatgatgccg acgactaacc caattagcgc atattcccag gtcatgaaca tctcccgttg
    32341 tcttttgttt ccgtagggtg ttggcttcag gctccatgcc tgcggctgat tatgccacta
    32401 taaccgttaa ttccacagaa gtggaatccc gactgcatat cgcgtagtgt agaacggcct
    32461 ttttttcgtc aaccgtgaac aacggcgcac cgattattca aggaataaca ataagatcat
    32521 gcaaagcgtt accccaacat cgcaatacct gaaggcgctc aatgaaggca gccatcaacc
    32581 cgacgacgtt caaaaagagg ccgtcagccg cctggaaatt atttatcaag aactcatcaa
    32641 tagcaggcca ccagccccca ggacgagtgg gctaatggcg cgggtcggta agctgtgggg
    32701 taaacgcgaa gacacaaagc atacgccagt gcgtggctta tatatgtggg gcggtgtagg
    32761 acgcgggaaa acctggctga tggacctttt ctatcaaagc ctgccgggag agcggaaaca
    32821 gcgcctgcac tttcaccgtt ttatgctgcg ggtgcacgaa gagctaactg ccttacaggg
    32881 gcagaccgat ccgctggaaa ttattgccga tcgctttaaa gccgaaactg acgtgctctg
    32941 ttttgacgaa ttttttgttt ctgatattac cgacgccatg ctacttggcg gtctgatgaa
    33001 agccctgttc acccgcggta ttaccctggt agcgacgtca aatattccgc cggacgaact
    33061 ttatcgaaat ggcctgcaac gtgcgcgttt tctgcctgca atcgatgcca ttaaacagca
    33121 ttgtgatgta atgaacgtgg acgctggtgt tgattatcga ctgcgtacac tcactcaggc
    33181 gcatctgtgg ctttcgccac tcaacgatga aacccgggcg cagatggata aactatggtt
    33241 ggcgctggcg ggggtgaaac gagaaaattc accgacgtta gaaatcaacc atcggccatt
    33301 ggcgacaatg ggcgtcgaga accagacgct ggcggtttct tttactacgc tgtgcgtcga
    33361 cgcccgcagt cagcatgact atattgcgct ctcacgcctg tttcacacag taatgttgtt
    33421 tgatgtgcca gtcatgacgc ggttgatgga gagcgaagcg cggcgcttta ttgcgctggt
    33481 ggatgagttt tacgagcgcc atgtcaaatt agtggtgagt gcagaagtgc cgctgtatga
    33541 aatttatcag ggtgagcggc tgaagtttga gttccagcgt tgcctgtcac gtctgcaaga
    33601 gatgcaaagc gaagagtatc tgaagcgcga gcatttggcg ggttaaaacc tgccacaaat
    33661 cacaaaaagg ggtcgatctt tgaccccgac ttctctataa tcctgcgacc ccacgttaca
    33721 agaaagtttt tttcccaaaa ctttttgtgt gctggcatag gctattcgaa ggggtaggtt
    33781 tgccggactt tgtcgtgtga acctcaacaa ttgaagacgt ttgggtgttc accaacgtgt
    33841 aactatttat tgggtaagct tttaatgaaa acttttacag ctaaaccaga aaccgtaaaa
    33901 cgcgactggt atgttgttga cgcgaccggt aaaactctgg gccgtctggc tactgaactg
    33961 gctcgtcgcc tgcgtggtaa gcacaaagcg gaatatactc cgcacgtaga taccggtgat
    34021 tacatcatcg ttctgaacgc tgacaaagtt gctgtaaccg gcaacaagcg tactgacaaa
    34081 gtgtactatc accacaccgg ccacatcggt ggtatcaaac aagcgacctt tgaagagatg
    34141 attgctcgcc gtcctgagcg tgtgattgaa atcgcggtta aaggcatgtt gccaaaaggc
    34201 ccgctgggtc gtgctatgtt ccgtaaactg aaagtttacg cgggtaacga gcacaaccac
    34261 gcggcacagc aaccgcaagt tcttgacatc taatcgggat tataggcaat ggctgaaaat
    34321 caatactacg gcactggtcg ccgcaaaagt tccgcagctc gcgttttcat caaaccgggc
    34381 aacggtaaaa tcgtaatcaa ccaacgttct ctggaacagt acttcggtcg tgaaactgcc
    34441 cgcatggtag ttcgtcagcc gctggaactg gtcgacatgg ttgagaaact ggacctgtac
    34501 atcaccgtta aaggtggtgg tatctctggt caggctggtg cgatccgtca cggtatcacc
    34561 cgcgctctga tggaatacga cgagtccctg cgttctgaac tgcgtaaagc tggcttcgtt
    34621 actcgtgacg ctcgtcaggt tgaacgtaag aaagtcggtc tgcgtaaagc acgtcgtcgt
    34681 ccgcagttct ccaaacgtta attggcttct gctccggcag aaaacaattt gcgaaaaaac
    34741 ccgcttcggc gggttttttt atggctaaat tctgaatcag cgtaaaaaat gggaagttgc
    34801 tttttgctgc cgtctgaaag acaggtaaaa caaaaaccac atcgccaata agggtctaag
    34861 tcaactattt cagactaaag cgcatctctt tttccccatt tccggcatcc actcaccaca
    34921 aaggtcgcaa aatctggtaa actatcatcc aattttctgc ccaaatgtcg ggtattgctc
    34981 attttttgtt tgattttcga acaaagagag tcgttcctta ttgggtaaca caacttctga
    35041 ctggccacct ggtggctggt agcagtaaaa attctgacta tacctggagg ttttcatggc
    35101 tgtcgctgcc aacaaacgtt cggtaatgac gctgttttcc ggtcctactg acatctatag
    35161 ccatcaggtc cgcattgtgc tggctgagaa aggtgtaagt ttcgagatcg aacacgtgga
    35221 aaaggacaat ccgcctcagg atctgattga cctcaacccg aatcagagcg ttccgaccct
    35281 ggtggatcgt gagctgaccc tgtgggaatc tcgcatcatt atggaatatc tggatgagcg
    35341 tttcccgcat ccgccactga tgcctgttta cccggtagct cgcggtgaaa gccgtctgta
    35401 catgcatcgc atcgaaaaag actggtacac gctgataaac actatcatca acggttcagc
    35461 ttctgaagca gatgccgcac gtaagcaact gcgcgaagaa ctgctggcga ttgcgccggt
    35521 cttcggtcag aagccgtact tcctgagcga tgagttcagc ctggtcgatt gctaccttgc
    35581 tccgctgctg tggcgtctgc cgcaactggg catcgagttc agcggcccgg gtgcgaaaga
    35641 gctgaaaggc tatatgaccc gcgtctttga gcgtgactct ttccttgctt ctttaactga
    35701 agcagaacgt gaaatgcgtc tgggccggag ttaatctgta tggatttgtc acagctaaca
    35761 ccacgtcgtc cctatctgct gcgtgcattc tatgagtggt tgctggataa ccagctcacg
    35821 ccgcacctgg tggtggatgt gacgctccct ggcgtgcagg ttcctatgga atatgcgcgt
    35881 gacgggcaaa tcgtactcaa cattgcgccg cgtgctgtcg gcaatctgga actggcgaat
    35941 gatgaggtgc gctttaacgc gcgctttggt ggcattccgc gtcaggtttc tgtgccgctg
    36001 gctgccgtgc tggctatcta cgcccgtgaa aatggcgcag gcacgatgtt tgagcctgaa
    36061 gctgcctacg atgaagatac cagcatcatg aatgatgaag aggcatcggc agacaacgaa
    36121 accgttatgt cggttattga tggcgacaag ccagatcacg atgatgacac tcatcctgac
    36181 gatgaacctc cgcagccacc acgcggtggt cgaccggcat tacgcgttgt gaagtaatac
    36241 aaaacaggcc caggcggcct gttttgtctt tttaatgact taataaaata gcgtgattgt
    36301 ggcaatcata ttcaccacga aaccgactgc catggggatc gctgtccgct tcaccaccgc
    36361 aaaaggcgaa acgcctgcaa ttcctgacgt aacgaccacc accgcagtaa tcggcgaaac
    36421 cgcgcgcgct agcgtcgtgg caaaatgcat cggcataatc attacaaccg ctggtacatg
    36481 tagtccggct gcgatattcg gaataagact ggcaaatgac ataaacggcg cattgccaga
    36541 gcccatcaca atggcacaaa tggcaatgac tagcgccata ataatcatca cgccaatacc
    36601 gcccagacca gaatgctccg cacccctgat aaccgcatca acagtgccaa tcgtcgttaa
    36661 gcctttcgca aatatttcac ccgcgaccac cagcgttacc acgttgacaa actgcgtacc
    36721 catgccgtca aaaaacgcct gcacatcgtc cattgtttcg cgcaagttat gcttgcggaa
    36781 gaactcaaca aacatcgtca ccgtcaaact tagtaacatc accaccacca gatgcagttc
    36841 tgacttcatc agcccgacgt gggcgaggaa cagcgagccg agcatcagga ttaacggcat
    36901 cacaggtaaa atggcgtaat agagcggcgg gacattatcg agagtttttt gctctgcctg
    36961 ttcgtgattg atatttttat cttttttgtc aaaagcgcgt tgcacgaaaa agtgggagat
    37021 cgccaccgag ataatgacgc aagaggcgac cggaagctgg tagtggaaga agtatgtggc
    37081 aattttcatt cccgcgacct gggctgcaaa aatggagttc gtttccagaa tcccccactc
    37141 aatggacatc gtggttgcga taaccgccac cgcagagagg cgacttactc ccagactcac
    37201 cagcgtcgga aataaggtga ccatcagcaa catacccaga ccggaggcgc tggtaataaa
    37261 ctgcgccatg atttggccga tgacgtaagt tgccgacaga ataatatacg gcgagcgaat
    37321 gagttttaac gggcggctta gcaagctcac catcgcgcga ctggctccta tgcgctccat
    37381 gtagcgggca taaccgccca ccgccataat cgacagcccc aatccggcaa tgcggttgga
    37441 cagcatgcgc aggatttcat tataaatgtc gacgatcaag tagccgctgc ttttggcggg
    37501 atcgagtaca ccaccgaacc ctaaccagac tccgcacatc atcaggaaga cacctgcggt
    37561 ttgtagtttt tcaggatcaa atagcccatc gtaattaata cgatgacaga tataattatg
    37621 ccgaac
//
LOCUS       DAETPZ010000031        36622 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.031, whole genome shotgun sequence.
ACCESSION   DAETPZ010000031 DAETPZ010000000
VERSION     DAETPZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36622
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.031"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(156..1391)
                     /locus_tag="K0W59_001799"
     CDS             complement(156..1391)
                     /locus_tag="K0W59_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBI3022864.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="K0W59_001800"
     tRNA            complement(1538..1614)
                     /locus_tag="K0W59_001800"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="K0W59_001801"
     tRNA            complement(1657..1743)
                     /locus_tag="K0W59_001801"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="K0W59_001802"
     tRNA            complement(1764..1839)
                     /locus_tag="K0W59_001802"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="K0W59_001803"
     tRNA            complement(1898..1974)
                     /locus_tag="K0W59_001803"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="K0W59_001804"
     CDS             complement(2077..3462)
                     /locus_tag="K0W59_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI3022865.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYITMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="K0W59_001805"
     CDS             complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="K0W59_001805"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HBI3022866.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="K0W59_001806"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="K0W59_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HBI3022867.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIFRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="K0W59_001807"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="K0W59_001807"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HBI3022868.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHACEFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTWMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="K0W59_001808"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="K0W59_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HBI3022869.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTVMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="K0W59_001809"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="K0W59_001809"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HBI3022870.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="K0W59_001810"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="K0W59_001810"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HBI3022871.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10723)
                     /gene="rfbA"
                     /locus_tag="K0W59_001811"
     CDS             complement(9860..10723)
                     /gene="rfbA"
                     /locus_tag="K0W59_001811"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBI3022872.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFDYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTDTHDSLIEASTFVQTVEKRQGFKIAWRNGWLDDEGVKRAASSLAKTGYGQYL
                     LELLRARPRQY"
     gene            complement(10742..11809)
                     /gene="rffG"
                     /locus_tag="K0W59_001812"
     CDS             complement(10742..11809)
                     /gene="rffG"
                     /locus_tag="K0W59_001812"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBI3022873.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGHYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11809..13071)
                     /gene="wecC"
                     /locus_tag="K0W59_001813"
     CDS             complement(11809..13071)
                     /gene="wecC"
                     /locus_tag="K0W59_001813"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HBI3022874.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13068..14198)
                     /gene="wecB"
                     /locus_tag="K0W59_001814"
     CDS             complement(13068..14198)
                     /gene="wecB"
                     /locus_tag="K0W59_001814"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBI3022875.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14254..15300)
                     /gene="wzzE"
                     /locus_tag="K0W59_001815"
     CDS             complement(14254..15300)
                     /gene="wzzE"
                     /locus_tag="K0W59_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HBI3022876.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15312..16415)
                     /gene="wecA"
                     /locus_tag="K0W59_001816"
     CDS             complement(15312..16415)
                     /gene="wecA"
                     /locus_tag="K0W59_001816"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HBI3022877.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16655..17914)
                     /gene="rho"
                     /locus_tag="K0W59_001817"
     CDS             complement(16655..17914)
                     /gene="rho"
                     /locus_tag="K0W59_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBI3022878.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(17999..18100)
                     /gene="rhoL"
                     /locus_tag="K0W59_001818"
     CDS             complement(17999..18100)
                     /gene="rhoL"
                     /locus_tag="K0W59_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HBI3022879.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18241..18570)
                     /gene="trxA"
                     /locus_tag="K0W59_001819"
     CDS             complement(18241..18570)
                     /gene="trxA"
                     /locus_tag="K0W59_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HBI3022880.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18701..19966
                     /gene="rhlB"
                     /locus_tag="K0W59_001820"
     CDS             18701..19966
                     /gene="rhlB"
                     /locus_tag="K0W59_001820"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HBI3022881.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20100..21584
                     /gene="gppA"
                     /locus_tag="K0W59_001821"
     CDS             20100..21584
                     /gene="gppA"
                     /locus_tag="K0W59_001821"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HBI3022882.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21631..23652)
                     /gene="rep"
                     /locus_tag="K0W59_001822"
     CDS             complement(21631..23652)
                     /gene="rep"
                     /locus_tag="K0W59_001822"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HBI3022883.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23739..24020
                     /gene="ppiC"
                     /locus_tag="K0W59_001823"
     CDS             23739..24020
                     /gene="ppiC"
                     /locus_tag="K0W59_001823"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HBI3022884.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24066..25541)
                     /gene="ilvC"
                     /locus_tag="K0W59_001824"
     CDS             complement(24066..25541)
                     /gene="ilvC"
                     /locus_tag="K0W59_001824"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBI3022885.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMVDWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25691..26581
                     /gene="ilvY"
                     /locus_tag="K0W59_001825"
     CDS             25691..26581
                     /gene="ilvY"
                     /locus_tag="K0W59_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HBI3022886.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26578..28122)
                     /gene="ilvA"
                     /locus_tag="K0W59_001826"
     CDS             complement(26578..28122)
                     /gene="ilvA"
                     /locus_tag="K0W59_001826"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HBI3022887.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDSHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28125..29975)
                     /gene="ilvD"
                     /locus_tag="K0W59_001827"
     CDS             complement(28125..29975)
                     /gene="ilvD"
                     /locus_tag="K0W59_001827"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBI3022888.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(30040..30969)
                     /gene="ilvE"
                     /locus_tag="K0W59_001828"
     CDS             complement(30040..30969)
                     /gene="ilvE"
                     /locus_tag="K0W59_001828"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HBI3022889.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(30989..31252)
                     /gene="ilvM"
                     /locus_tag="K0W59_001829"
     CDS             complement(30989..31252)
                     /gene="ilvM"
                     /locus_tag="K0W59_001829"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HBI3022890.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31249..32895)
                     /locus_tag="K0W59_001830"
     CDS             complement(31249..32895)
                     /locus_tag="K0W59_001830"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HBI3022891.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAIAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32898..32948)
                     /locus_tag="K0W59_001831"
     CDS             complement(32898..32948)
                     /locus_tag="K0W59_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022892.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33034..33132)
                     /gene="ilvL"
                     /locus_tag="K0W59_001832"
     CDS             complement(33034..33132)
                     /gene="ilvL"
                     /locus_tag="K0W59_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HBI3022893.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33484..35004
                     /locus_tag="K0W59_001833"
     CDS             33484..35004
                     /locus_tag="K0W59_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBI3022894.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(35029..35367)
                     /gene="maoP"
                     /locus_tag="K0W59_001834"
     CDS             complement(35029..35367)
                     /gene="maoP"
                     /locus_tag="K0W59_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HBI3022895.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35486..36325
                     /gene="hdfR"
                     /locus_tag="K0W59_001835"
     CDS             35486..36325
                     /gene="hdfR"
                     /locus_tag="K0W59_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HBI3022896.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLSGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGSLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36421..36496)
                     /locus_tag="K0W59_001836"
     tRNA            complement(36421..36496)
                     /locus_tag="K0W59_001836"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36461..36463),aa:Trp,seq:cca)
     gene            complement(36505..36581)
                     /locus_tag="K0W59_001837"
     tRNA            complement(36505..36581)
                     /locus_tag="K0W59_001837"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36545..36547),aa:Asp,seq:gtc)
BASE COUNT         8946 a        10027 c         9224 g         8425 t
ORIGIN      
        1 gccaccgcac gcaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccaccctgcc aagtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gccatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgccgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttatgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgcaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa cgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaaa attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacgcc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccac gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgcg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cacgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcataacggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atcttaaagc cctggcgttt ttctactgtc tgcacaaaag
    10021 tgctggcctc aatcaggctg tcgtgagtgt cggtatccag ccaggcaaat ccgcgcccga
    10081 gcagttcaac ggtcaggttg cccgcctcga ggtacatctg gttgatggag gtaatctcca
    10141 gttcaccgcg ctccgacggc ttcacctgct ttgcgtactc cacgacttta ctgtcgtaga
    10201 aataaagccc ggtcaccgcc cagtttgact tcggctgttt tggcttttct tcaagcgaga
    10261 tagcgcggaa attgtcgtca aattccacca cgccaaagcg ttccgggtcc atcacctgat
    10321 agtcaaaaac cgtcgcccct tccgtgcgcg ccgcaacatg acgcagcttc ggactgaagc
    10381 cctgaccgaa gaagatgtta tcgcccagca ccagacaaga aggttcacca ttaaggaagg
    10441 tttcaccgat gataaaggcc tgcgccagac cgtccgggct gggctgttcg gcatattcca
    10501 gctggatacc gaactcacta ccatcgccca gcaggcgctg gaaataacct ttatcttccg
    10561 gcgtagtgat gatgagaatt tcgcggatac cggccagcat cagcaccgac agcggatagt
    10621 aaatcattgg cttatcgtaa atcggcaaca gttgcttcga tacgccgcgc gtaatcggat
    10681 gcaatcgggt accggaaccg cccgccagga taataccctt catgtttgcc tccgctgaaa
    10741 attagccttt cagacctaaa cgctcaccct gatagctgcc gtcctgcacc tgcttccacc
    10801 agctttcatt agccagatac cactgaaccg ttttacgcat tccactttca aaggtttcct
    10861 gcggcagcca gccaagttca cgggcaattt tcgaagcatc aatggcataa cgcagatcat
    10921 gccccggacg gtcagcgaca aaggtgatca ggtcacgata gtgcgccacg ccgtgcggct
    10981 tgttcgaagc cagttcttcc agcagctcgc aaatggtttc cacaacatcg agattcttac
    11041 gctcgttgtg accaccaata ttataggttt caccgacttt cccggtggtc gccacgcaat
    11101 acagcgcgcg ggcgtgatct tccacataca gccagtcacg gatttgctgc ccgttgccat
    11161 ataccggcag cggtttaccc gccagcgcgt tgaggatcat cagcgggatc agtttttccg
    11221 gaaagtggta atgaccgtag ttattcgagc agttggtgat cagcgtcggc agaccgtagg
    11281 tccgcagcca ggcgcgcacc agatggtcgc tgctggcttt tgacgcggaa taagggctgc
    11341 tcggcgcata cggcgtggtt tcggtgaaga aatcatccgt cgagtgcagg tcaccatata
    11401 cttcgtcggt ggagatatga tgaaaacgga acgctgattt tttatcttcc gtcagcgtat
    11461 tccagtaagc ccgcgccgct tcaagcaatg tataagtccc gacaatgttg gtttcaataa
    11521 acgctgccgg gccgtcaata gaacggtcaa catggctttc ggctgccaga tgcatgacac
    11581 agtctggttg atgctcagtg aatacgcgtg ccagttctgc ccgatcgcag atatcaactt
    11641 tctcaaaggc aaagcgctcg ctttgcgcga ccggtgccag cgacatcagg tttccggcgt
    11701 aggtcagctt atcgaccact accaccgcat cgctcgtttc gttgatgata taacgcacca
    11761 gcgccgagcc aataaacccg gcaccacctg ttatcagaat ttttctcatc agcgccagac
    11821 tcctttggca tcgacgacat actgctgatg gacattgtcg ccattgataa ctttgaactg
    11881 actatgatcg accagcatca ccagcacgtc tgccgttgcc agcgcctcgt caagctgcgc
    11941 cagagtacaa agcccggtca gttttttcgg caactggtgg atgttaggct caacaaccag
    12001 cgtttcgccg ctgtgccact gggcgatcag ttcagcgatt tccatcgccg ggctttcgcg
    12061 caggtcatca atattcggtt taaacgccag accaaagcag gcgattttca gttcactggc
    12121 gcgtttatcg gtagcagcaa ggcagtcggc aaccgccgct ttcacctgat cgataaccca
    12181 gaacggtttg tgatcgttaa cttcgcgcgc ggtacggata agccgcgcct gctgggggtt
    12241 ctgtgccacg ataaaccacg gatcaacagc aatgcagtga ccgcccacgc cagggccagg
    12301 ctgaagaata ttaacgcgag ggtgacgatt cgccaggcga atcagttccc agacgttaat
    12361 cccctgatcg gcacaaatca gcgacaattc attagcaaaa gcgatattca catcgcggaa
    12421 gctgttttcg gtgagcttac acatttccgc cgtccgcgag ttagtgacga cacactcacc
    12481 ttcgaggaaa attttgtaca attcgctggc gcgggccgaa caaaccggcg tcataccacc
    12541 aatcacgcga tcgtttttaa tcagctcgac cataacctgg cccggtaata cacgttccgg
    12601 gcagtaagca atgttgacgt ccgcctgctc gcccacctgc tgtgggaaag tcagatccgg
    12661 gcgcatctct gctaaccatt cagccatctt ctcggttgac cccaccggcg aggtggattc
    12721 aaggatcacc agggcgcctt ttttcagcac tggcgcaatg gagcgagcag ccgattcaac
    12781 gtaggtcata tctggctcat gatcgccttt aaacggcgta ggtacagcaa tcagccaggc
    12841 atccgcttca actggcgtcg tgctcgctcg taaaaaaccg ccttctacgg cagtttttac
    12901 tacgctcgcc aaatcaggct cgacgatatg gatttcgcca cgattgatgg tatcaaccgc
    12961 gtgttggttg atatcgacac caatcacctg tttttgccgt gaggcaaacg ctgctgctgt
    13021 aggcagcccg atgtaaccca gtccgataac agaaatggtc gcaaaactca tagtgatatc
    13081 cgattatttt ttaacgcttc cagaatgcga gagcatgcct gaccatcacc atacgggtta
    13141 tgggcgcggc tcatagcttg atattcgttt tcgtctttta aaagacgcgt cacttcctcg
    13201 acaattcgct gcttatccgt gcctaccaga cgcaccgtac ccgccgtcac cgcttccgga
    13261 cgctcagtgg tatcgcgcat caccagcacc ggtttcccca gcgaaggcgc ttcttcctga
    13321 atgccgcctg agtcagtcaa aatcagccag gcgtggttca tcagccagac aaacggtaaa
    13381 tactcctggg gatcgatcag aatgacattt ttcacatgcc ccagaatgcg attgaccggc
    13441 tctctgacgt tcgggttgag atgcaccgga tagacaatct ggatgtcctg gtgcgtggtg
    13501 gcgatgtctg ccagcgcgtg gcagatttct tcaaagccac gaccgaaact ctcacgccta
    13561 tgaccggtca ccagaatcat ctttttatcg ggggcgataa acgggtaatt tgccgccagt
    13621 tctgaacgca gcgtgtcgct gctcatcacc tggtcacgca cccataacag tgcatcaatg
    13681 actgtattac cggtaatgaa gattcggcta tccgcaacgt tttcacgcag caagttttgc
    13741 cgggaagttt cggttggaga gaagtgatac atcgccagat gcccggtcaa tgtacggtta
    13801 gcctcttccg gccacggcga atagagatcg cccgtgcgca gaccagcctc aacgtgacca
    13861 acaggaatac gctgataaaa cgccgccagg ctggttgcca gcgtcgtcgt cgtatcgccg
    13921 tgaaccagca caacgtctgg tttgaactcg gcaagaatag gttttagccc ttccagaatc
    13981 cgacaggtta tctctgtcag gccctgtcct ggctgcatta tgttgagatc gtagtcaggt
    14041 acaatggaaa agagtttcag tacctgatca agcatctccc gatgctgcgc agtgacgcaa
    14101 actttagcct caaaaaaagg atcttttgcc aacgcatgca ccaacggagc catcttgatg
    14161 gcttccgggc gcgtaccaaa tacagtcagt actttcacat cgattctctt cgaataagcg
    14221 gcgagcgcct ttgcgctcac cgcagcagtg ttgctatttc gagcaacggc gggttaatgc
    14281 gacaccagcc ccgatcagcc ccccgacaat gccccacata atcatcagga aggcacgacg
    14341 tgggctatcg cgttttaccg gttcttccgg cgtacgcaaa tagcgatagg tctgaaaacg
    14401 cggatccagg gttggaccaa cattcagggt gtttaacatg gctcgattct gatcatagtc
    14461 gagatcaaag gccggaccga cggcctgtaa attttccagt cgagcctgga gcattggacg
    14521 cccaagcagg aacatttctg aatcaggtaa ttcctcggca ggcacatctg tcgcactgcg
    14581 cgaaatatta tgctgctcag caattttcag cgcctgctca atgctgttca tccggcggtc
    14641 gtagatggct ttcgccacct cttcctgacg cttcacctga gctttcatct ggatggtgcg
    14701 tgccgcccat gcgcctttca gctcatcatt cagatggctg gctgcacgct ggctggcaaa
    14761 agcaacatac tgacgtaaca ggttattagc gtcaggcgcg gtttcggcaa taagcttcac
    14821 gctgtcattg accgcgcgag taaagtctcc ggggataaac tggatgttgt taatcatttc
    14881 atccagcaac gccgcatcgg ctttgctgtt gcccaccatc cgctgtttgt aatagtcggt
    14941 ttgcagccag aactctctgc gggtatccca cgaggccagt tgcataacaa actctttata
    15001 ggcttcgtcc atgaccgatg gttgatcggc agaagccatg tttgaacgga catccaggtt
    15061 acgcaaaaat tgctgctgcg agtaatatcc ccccagcata ttcaccgttg gacgatcggt
    15121 aatcgccgtc gagctccact cctgacgagc aaaaaaagta tacgccagcg cgattaacgc
    15181 aaacgccagc cccatgccaa taatccatag cttcccagcc cacaaggtac gaaacaaccc
    15241 acgaatatcc agttcatttt cagcgtcttc ggccggtttc ccaggcattg gttgtgtcat
    15301 cacatcctca tttatttggt taaattgggg ctgccaccac gatttctacg cagtctgcgt
    15361 tttacgcgct taataaagcg agcaactttc caggcacgct taatgcaata tccatagagg
    15421 aagaatgcta gcaaaaagag caccagcatg acccactccg ggacaaaatg agaatattct
    15481 gccagcacgc caatggaagc gagcagtgct gcggcaaggg taatcagcac aaacgcctga
    15541 cgggaagtaa acccggcacg catgatcaaa tggtgaatat gctggcggtc aggagagaat
    15601 gggctcatgc ctttacgcag gcgacggtac ataatcgcca ccatatccat tagcggaatg
    15661 gcgattatcc acaaagcggt aaccgggctg atgggatggg ttttgccctg ggtcgtttcg
    15721 agcaggatcc agataacggt aaaaccaatc agcgtactgc ccgcatcacc cataaagact
    15781 ttgtagcggc gacccaggat accaaggtta agcatgatgt atggcaggat ggcggcgatc
    15841 atcgcaaagc accagattgc gaggctggtt tgcccgtcga accacaaaat cataccgatt
    15901 gctgcaaacg agacgcagga caacccgccc agcaagccat caatgccatc aaccatgttg
    15961 aacgcattaa tggccgccca gacggcaaat agcgtcagga agtaaccaaa cggtccgagc
    16021 accatctccc aggagccaaa gatataaccc aggctactga gataaagttt gccgaacacc
    16081 atcataacaa tgccaacagc ggcctgtatg gtggcacgga tttttacgct gatatcaaaa
    16141 cgatcatcca gcgcgccaat gaaaacaagc acaccggcac aagcgagata gagagatgca
    16201 tgcggaatat agtaatcgac aattccgaac gtgaagcaaa tccctgcgta aaccgaaatc
    16261 cccccaacga gaggtatcaa tccctggtga cgtttgcgga agtttggttt atccactaaa
    16321 ccgacttttt ttgccacctt acgggcaaaa aacagaaaca gtgtcgtgaa taaaaaaata
    16381 ctgatgagat cagtactcac tgtcagtaaa ttcacaatgc atgctctcag agaaaattat
    16441 tagcagaagt ataaccacga agacctttat tcagaaggga aagtccgttc cgaacccacc
    16501 agccctgtgc aaaaattaac catttgatat gcaaattgca taacatgcca gcaaattcca
    16561 ggaattttcc taacatcgcc agcgcgacat taagattaca gatataaaag caaaacgcca
    16621 cgtaaacacg tggcgttttt ggcataagac caatttatga gcgtttcatc atttcgaaga
    16681 aatcgtcatt ggtcttggtc atggccagtt tattaatgag gaattccatt gcatcgattt
    16741 cgcccatcgg gtgaatgatt ttgcgcagga tccacatttt ctgcagttct tcctgagtcg
    16801 tgagcagctc ttctttacgg gtaccggaac ggttgtagtc gatagccggg aagacgcgtt
    16861 tttcagcgat cttacgagag aggtgcagtt ccatgttgcc tgtaccttta aactcttcgt
    16921 aaataacttc gtccatttta gaaccggtat cgataagcgc cgtcgcgata atggtcaggc
    16981 tgccgccctc ttccacgtta cgcgccgcac caaagaagcg tttcggacga tgaagggcat
    17041 tggcgtccac accaccggtc aacactttac ctgacgccgg aacaacagtg ttgtaagcgc
    17101 gcgccagacg agtgatggag tcgagcagaa tgataacgtc tttcttgtgc tcaaccaggc
    17161 gtttggcctt ctcgatcacc atttccgcaa cctgaacgtg gcgagatgcg ggttcgtcaa
    17221 aggtagaagc aacaacttca ccttttacca gacgctgcat ctcggttact tcttccggac
    17281 gttcgtcgat cagcagaacc atcagcacac aatccgggtg gttgtaagca atgctctgag
    17341 caatgttctg cagcagcata gttttaccgg ctttcggcgg tgccacaatc agaccacgct
    17401 gaccacgacc gataggtgat gccagatcca gtacgcgagc agttaaatct tcagtagaac
    17461 cgttaccacg ttccatacgc agacgagagt ttgcgtgcag cggggttaag ttctcaaaga
    17521 ggattttgtt gcgggcgttt tcaggtttgt cgaagttaac ttcgttaact ttcagcagcg
    17581 caaaatagcg ttcaccttct ttcggcgggc gaatcttacc agagatggta tcaccagtgc
    17641 ggaggttgaa acggcggatt tggctagggg aaacgtagat gtcatcagga ccggcgaggt
    17701 aggagctgtc tgcggaacgg aggaaaccaa atccatcctg caatatctcc agtacgccat
    17761 caccaaagat atcttcgcca ctctttgcgt gctgcttcag gatggcaaaa ataatgtcct
    17821 gcttacgcat gcgagccagg ttttccagcc ccatattttc gccgagagtg atcagctcag
    17881 aaaccggcgt attctttaat tcggtaagat tcatagtggt gtgagttctt aaacttgggg
    17941 taaatctcga acttaatgtt gtgaatggta tggcaggatc atccatgcct gtttacggtc
    18001 atcgactcat gtcttttcgc tgcctggtca caggagagta cgcagaactg aaacgacaag
    18061 acggattgag tgacgagcca gaaatctgtt cacttcgcat ttaagataaa acgggaagcg
    18121 ttggaaatta caagattcaa acttaataag gtatgtttaa tacgaagtca acactaagtt
    18181 agcatgactc acgccgggcg tccagttttt agcgacgggg cacccgaaca tgaaattccc
    18241 ttacgccagg ttagcgtcga ggaactcttt caactgacct ttagacagtg cgcccacttt
    18301 ggttgccgcc acttcaccgt ttttgaacag cagcagagtc gggataccac ggatgccata
    18361 tttcggcgca gtgccagggt tttgatcgat gttcagtttt gcaacggtca gtttgccctg
    18421 atattcgtca gcgatttcat ccagaatcgg ggcgatcatt ttgcacggac cgcaccactc
    18481 tgcccagaaa tcgacgagga tcgccccgtc cgctttgagt acatccgtgt caaaactgtc
    18541 gtcagtcagg tgaataattt tatcgctcat atataactcc acaggaataa gcctggcgtg
    18601 ttggtttcgt tgttggtgta acattaacca actaaaggtt gactttattt caccggatac
    18661 gctttcgtaa agcaatagta agctgatatt ctaccacact atgagcaaaa cacatttaac
    18721 agaacagaag ttttccgact tcgccctgca tccgaaggtt gtagaagccc ttgaaaaaaa
    18781 agggtttcat aactgtacgc ccattcaggc actggccctt ccgctgacgc tggcgggtcg
    18841 tgacgtagcc gggcaggcgc aaaccggtac cgggaaaacg atggcgtttc ttacgtcaac
    18901 gtttcattat cttctctctc atcctgcgat tgccgatcgc aaggtgaatc aaccgcgtgc
    18961 cttaattatg gcaccgacgc gtgaacttgc cgtgcagatc cacgccgacg cggaaccgct
    19021 ggcggaagct accggcctga agctgggcct ggcttacggt ggtgatggct atgacaaaca
    19081 gctgaaagtg ctggaaagcg gcgttgacat tctgattggc accacgggtc gtttaattga
    19141 ctacgccaag cagaaccaca ttaacctcgg tgccattcag gtagtggtac tggacgaagc
    19201 cgatcgcatg tacgatctag gctttattaa agatatccgc tggctgttcc gccgtatgcc
    19261 gcctgcaaat cagcgcctca acatgctgtt ctccgccacg ctttcgtacc gggtacgtga
    19321 actggcgttc gagcagatga acaatgccga atatattgaa gtggaaccgg aacagaaaac
    19381 gggccaccgt attaaagaag agcttttcta cccttctaac gaagaaaaaa tgcgtttgct
    19441 gcaaacgctg atcgaagaag agtggccaga cagagcgatt attttcgcca ataccaaaca
    19501 ccgttgtgaa gagatctggg gccacctggc ggcagatggt catcgtgtcg gtttgttgac
    19561 aggcgatgtc gcgcagaaaa aacgtctgcg tattcttgat gaatttaccc gtggcgatct
    19621 ggatattctg gttgccaccg acgttgccgc gcgtggtttg catattccgg cagtgacgca
    19681 cgtctttaac tacgatttac ccgatgactg tgaagattac gttcaccgta ttggtcgtac
    19741 aggtcgcgca ggcgcaagcg gtcactctat cagcctggcg tgtgaagagt atgcattgaa
    19801 tttgcctgct attgagacct atattggtca ctcaattccg gtaagcaaat acaatccgga
    19861 cgcattgatg accgatctgc caaaaccgct gcgcctcacg cgcccgcgca caggcaatgg
    19921 tccgcgtcgt actggcgctc cgcgtaatcg tcgtcgttca ggttaaataa aatgtcatac
    19981 tcaaaatcat tcaaactgta tcaagatggc aagagaatga atcccgatga gcttactgta
    20041 gtaagtgatt cgggtgaaag aatgcagcca acacagagac agattgaagg atgaagagta
    20101 tgggttccac ctcgtcgctg tatgcagcca ttgatctcgg ttcgaatagt tttcatatgc
    20161 tggttgtgcg cgaggtggct ggaagcatcc agacgctgac gcgaattaaa cgcaaagtgc
    20221 gtctggctgc tggcctgaat agcgaaaatg acctctctaa tgaagcaatg gagcgcggtt
    20281 ggcaatgtct gcgcctgttt gctgaacgtc tgcaagatat ccctccctcg caaattcgcg
    20341 ttgtcgctac ggcgacatta cgacttgccg tcaatgcggg tgattttatt gccaaagcgc
    20401 aggaaatcct cggttgtccg gtacaggtga tcagcggtga agaggaagcg cgtctgattt
    20461 atcagggcgt tgctcacacc actggtggtg ccgatcagcg cctggtggtg gatataggcg
    20521 gtgccagtac tgaactggta accggcacgg gtgcacaaac cacctcgttg ttcagcctgt
    20581 cgatgggctg cgtcacctgg ctggaacgct attttgccga tcgtaatctg gggcaagaaa
    20641 attttgatgc tgcagaaaaa gcggcacgcg aagtgttacg tccggttgcc gatgaattac
    20701 ggtatcacgg ctggaaagtg tgcgttggtg cttccggcac cgtgcaggcg ttacaggaaa
    20761 tcatgatggc acaggggatg gatgaacgca ttaccctgga aaagttgcag caattgaaac
    20821 agcgagccat tcattgcggt cggctggagg aactggagat tgacgggctg acgctggaac
    20881 gtgcgttagt gttcccgagt ggtctggcga tcctgatcgc catttttacc gaactgaata
    20941 ttcagtgtat gaccctggcg ggcggtgcgc tgcgtgaagg cctggtctac ggcatgttgc
    21001 atctggccgt cgagcaggat attcgcagcc gtacactgcg taatattcaa cgccgcttta
    21061 tgatcgatat tgatcaggca cagcgcgtag ccaaagttgc ggctaacttc ttcgatcagg
    21121 tggaaaacga atggcatctt gaagcaataa gccgcgattt gctcatcagc gcctgtcagc
    21181 ttcatgaaat cggcctgagc gttgacttca aacaagctcc gcaacacgct gcttatctgg
    21241 tgcgtaacct ggatcttccc ggttttaccc ccgcacagaa gaaactgctg gcgacgctac
    21301 tgctcaacca gactaacccg gtcgatctct catcgctgca tcagcaaaat gccgtaccac
    21361 cgcgcgtcgc agaacaactc tgccgtttac tccgcctggc tatcattttt gccagccgtc
    21421 gccgtgacga tctcgtgcca gagatgacat tacaggctaa ccatgaactg ttgaccttga
    21481 cgcttccgca aggttggcta acccaacatc cgctgggtaa agagattatt gctcaggaaa
    21541 accagtggca gagctatgtc cactggccgc tggaagtgca ttaatgagta agtgccggat
    21601 gcgatgctga cgcatcttct ccggccttga ttatttccct cgttttgccg ccatcatcgc
    21661 tttcagattc gccagatggc tttgcccttt ctgcatccgt tcttcggcac tgaccacttt
    21721 gcgctcctgt tcccaaatca gatcatcctg cggcagctcc agcaaaaagc ggctcggctc
    21781 cgggcgcacc agttcgccgt actgacggcg ttctttacac agcgtaaagg tcaattcctt
    21841 ctgggcgcgg gtaatgccga cataggccag ccgccgctcc tcatcgatat tatcttcatc
    21901 gatactgctc tggtgcggca aaaacccttc ttccataccg accatgtaga cataaggaaa
    21961 ctccagccct tttgacgcat ggagagtcat cagttgcacc tgatccagct cttcttcact
    22021 ctcaccacgc tccatcatgt cgcgtaaagt aaagcgcgtc accacctggg tgagcgtcat
    22081 cggctcatcc agttcgctgc cttccagcat ctccgtcatc cagctaaaca gttggttgac
    22141 gttcttcatg cgcatttcgg cagctttcgg gctgggcgat gtttcgtaca gccaggattc
    22201 ataatccatg ccgtggatca gatcgcgcac cgcggcaatc ggctcccgct ccgccagacg
    22261 ctggatttct gccaaccagt gagtgaagcg ggtcaatgct tcataaccac gtccgctaag
    22321 cgtctgactc aggcccatat caaagctggc ggtaaacatg cttttattgc gcgtcatcgc
    22381 ccactcaccc agctttttca gcgtcgccgg gccaatctct cgcttcggcg tgttaacgat
    22441 acgcagaaat gcgctgtcat cgtccgggtt agtcagcacg cgcagataag ccagcaagtc
    22501 cttgatttca ggacgagaga aaaacgacgt accaccagaa attttgtacg ggatgcggtt
    22561 ttgcatcagg aatttttcaa acacccgcga ctgatggtta ccgcgataaa gaatagcgta
    22621 atctttgtac tgcgttttat tgacgaagtg atgcgcaatc agttcaccag tgacgcgctc
    22681 ggcttcatgt tcttcgttat tcgcgcttaa aacttttagc tctgtgccgt aacccagttc
    22741 ggagaacaga cgcttttcaa agacgtgcgg gttattggcg atcaggatgt tcgccgcttt
    22801 caaaatacgt ccggaagagc ggtagttctg ctcaagctta atcaccttca gcgccggaaa
    22861 atcctggctc agcagcacca ggttttgcgg acgtgcgccg cgccaggagt agatcgactg
    22921 gtcatcgtca cccaccacgg taaagcgcgc gcggctgccc accagcagtt tcaccagttc
    22981 atactggctg gtgttggtgt cctgatattc gtccaccagc aggtagcgaa ttttgttctg
    23041 ccagcgctcg cggacttctt cattgcgttg cagaagtagc gtcggcaata aaatcagatc
    23101 atcgaagtcg agcacattac aggctttcag gtgtgcatca tacagcccat aacaatgggc
    23161 aaaaatacgg tcccgctcgc caatcgcact ggctgccgcc tgggacggtg ttttgagatc
    23221 gttcttccag ttagagatgg tcgaaatcaa ctgttgcagg agaactttgt catcttcaat
    23281 cagcccttcg gtcaactctt tgagcaacgc cagctgatcg gtatcgtcaa acagcgaaaa
    23341 gttcgccttc atccccagcg ccgcatactc acgcttgatg atatccagcc ccagcgtgtg
    23401 gaaggttgag atcatcagcc cacgcgcctc tttgcgaccc agcgtctgtc cgacacgctc
    23461 tttcatctcg cgcgccgcct tattggtaaa ggtcaccgcc gcgatatgtc gcgcctgata
    23521 accgcacccg cggatcagat gggcgatttt attggtgata acacgagttt taccggaacc
    23581 cgcacccgcc agcaccaggc aggggccggt aacgaattcg acagcttgtt gttggccggg
    23641 gtttagacgc ataggaaaat cgctcaatga aagtcgggag gggaaaataa cagcgtggta
    23701 gtatagccag cctcatcctt accactcaag gcacgatcat ggcaaaaaca gcagcagcac
    23761 tgcatatcct tgtaaaagaa gagaaactgg ctctggatct tctcgtgcag attaagaacg
    23821 gtgccgattt cggcaagctg gcgaagaaac actccatttg cccatcaggc aaacgcggcg
    23881 gtgatttagg tgaattccgc cagggtcaga tggttccggc gttcgataaa gttgtgttct
    23941 cttgtccggt actggagcca accggcccgc tgcacaccca gttcggttac cacatcatta
    24001 aggtgctgta ccgtaactaa tagcaaggcc ttctccggga gaaggccttg aactctgaat
    24061 cgctattaac ccgcaacagc aatacgtttc atatccgtca tatagccgcg cagtttctta
    24121 cctacttgct caatcgcatg gctgcgaatc gcttcattta catcacgcag ctgcgcgtta
    24181 tctaccgcac cttccggaat agctttaccc aaatcgcccg gttgcagttc tgccataaac
    24241 ggtttcagca gcggcacaca agcgtaagag aacagatagt taccgtactc ggcagtatca
    24301 gagataacca cgttcatttc atacagacgc ttacgggcga tggtgttggc aatcagcggc
    24361 agttcgtgca gtgattcata gtaagcagat tcttcgatga tgccggaatc aaccatggtt
    24421 tcgaacgcca gttcaacgcc cgctttcacc atcgcgatca tcagtacgcc tttatcgaag
    24481 tactcctgct cgccgatttt gccttcatac tgcggcgcgg tttcgaaggc ggttttgccg
    24541 gtctcttcac gccaggtcag cagtttctta tcatcgttgg cccagtccac catcataccg
    24601 gaggagaatt ctccggagat gatgtcgtcc atatgtttct ggaacagcgg tgccatgatc
    24661 tctttcagct gttcagacaa cgcgtaagca cgcagtttcg ccgggttaga gagacggtcc
    24721 atcatcagtg tgataccgcc ctgcttcagc gcttcggtga tggtttccca accgaactga
    24781 attagttttt ctgcgtatgc cggatcggtg ccctcttcca ccagcttgtc gaagcacagc
    24841 agcgagccag cctgcaacat accgcacagg atggtttgct cacccatcag gtcagatttc
    24901 acttccgcaa cgaaggacga ttccagcacg cccgcacggt gaccaccggt tgctgccgcc
    24961 catgctttag cgatcgccat gccttcgcct ttcggatcgt tttccgggtg aacggcaatc
    25021 agcgtcggta cgccgaatcc acgtttgtac tcttcacgca cttcggtgcc agggcatttc
    25081 ggcgcaacca tgactacggt gatgtcttta cggatctgct cgcccacttc gacgatgttg
    25141 aaaccgtgcg agtaacccag cgccgcgccg tctttcatca gtggctgtac ggtgcgcact
    25201 acatcagagt gctgcttgtc cggcgtcagg ttaaccacca gatccgcctg tgggatcagt
    25261 tcttcgtaag tacccacttt aaaaccattt tcggtcgctt tacgccagga cgcgcgcttc
    25321 tcggcaatcg cttctttacg cagagcgtag gagatatcga gaccagaatc acgcatgttc
    25381 agaccctggt tcagaccttg tgcgccacag ccgacgatga ctactttttt accctgaagg
    25441 tagctcgcgc catcggcgaa ttcatcgcgc cccataaagc gacatttgcc cagctgtgcc
    25501 agctgctgac gcagattcag tgtattgaag tagttagcca tggtgattcc tcgtgatgtt
    25561 gtgcttctta ttgttcggtt cgcgtttgcg agattgaatt cactatatga caggaaattt
    25621 attgcggaaa ttgatatatt cacaacgtca cattgcaatt tctgcaacgt caacattgag
    25681 ggctgcgcca ttggatttac gcgatctgaa aaccttcctg catctggcgg aaagccgcca
    25741 ttttggccgc agcgcgcggg cgatgcacgt tagcccgtcc acgctctcac ggcagattca
    25801 gcgcctggaa gaagatctcg gccagccgct gtttgtgcgc gataaccgca cggtgacgct
    25861 gactgaagcg ggcgaagaac tgcgcgtttt cgcccagcaa acgctgttgc agtatcagca
    25921 gctgcgccac accatcgacc agcaagggcc gttgctctat ggcgaattac atatcttctg
    25981 ctcggtaacc gccgcttaca gccatctgcc gccgattctg gatcgcttcc gcgcggaaca
    26041 cccgtcggtg gagatcaaac tcactaccgg tgatgcggca gatgcaatgg aaaaagtcgt
    26101 cacaggtgaa gctgatctgg cgattgccgg gaagccagag actctgcccg gcgcggtggc
    26161 gttttcgatg ctggagaatc tggcagtggt gttgattgca cccgcattgc cctgcccggt
    26221 gcgtaatcag gtttctgtcg aaaagccgga ctggtcaacg gtaccgttta tcatggccga
    26281 tcaggggccg gtgcgccgcc gcattgagtt gtggttccga cgcaataaaa tcagtaaccc
    26341 gatgatttac gccacggttg gcgggcatga agcgatggta tcgatggtgg cgctcggctg
    26401 tggcgtggca ttgttgccgg aagtggtgct ggaaaacagc cccgaaccgg tgcgtaaccg
    26461 cgtgatgatt ttagagcgca gcgatgagaa aacgccgttt gagctgggcg tgtgtgcgca
    26521 gaaaaagcgg ctacatgagc cgctaattga ggcattctgg aagattttgc cgaaccatta
    26581 acccgccaaa aagaacctga acgccgggtt attggtttcg tcgtggcaat cgtagcccag
    26641 ctcattcagc cgggtttcga aatccggttc atggtcgcca agttcgaacg ccgccagtac
    26701 gcgcccgtag tcggtgccat ggctgcgata gtggaacaaa gaaatgttcc agtgcgtacc
    26761 cagcgtgttg aggaagcgca gcagcgcgcc cggtgattcc gggaattcga agctgtagag
    26821 gcgttcctgc aacggatgtg acggacgccc gccgaccata tagcgcacat gcaacttcgc
    26881 catttcgtcg tcggagagat ccaccacgct gtagccgccg tcgttgagca tctgcaaaat
    26941 ttctttgcgc tcttcgaggc cgcggctcag gcgcacgccg acaaagatgc aggcgttttt
    27001 ggcatcggca aaacggtagt tgaactcggt gaccgatcgc ccgccaagca gttggcagaa
    27061 tttgaggaag ctgccttttt cttccggaat ggtcaccgcc aacaacgctt cacgctgttc
    27121 gcccagttcg cagcgttccg agacgtagcg caggccgtgg aagttcacgt tggcaccgga
    27181 aagaatatgc gccagccgtt cgccgcgaat gttgtgctgg gcgatatatt ttttcattcc
    27241 cgccagcgcc agcgcgccag agggttccgc caccgcacgc acatcttcga ataaatcctt
    27301 catcgccgca cagatcgcat cgctatcgac ggtgatgatg tcgtcgagat actcctggca
    27361 taaacggaag gtttcgtcac cgatgcgttt taccgcgaca ccttcggcga acagtcccac
    27421 gcgtggcaga tcaaccggat gacccgcatc cagcgctgct ttcaggcagg cggagtcttc
    27481 cgcttctacg gcgatcactt tgatttgcgg catcagttgt ttgatcagca ccgctacgcc
    27541 cgccgccaga ccgccgccgc cgactggcac aaatacgcgg tcgagatggg agtcctgctg
    27601 gagcagttcc agcgccagcg tgccttgccc ggcaatcacc atcggatggt cgaacggcgg
    27661 cacccaggtg aacccctgct gctgtgacag ttcgatcgct ttagctttcg cttcgtcaaa
    27721 gttcgcgcca tgaagcagca cttcgccgcc gaatccacgt accgcatcga ctttgatgtc
    27781 ggcggtggcg gttggcataa cgatcagggc cttcacgcct aaccgcgcag aagaaaacgc
    27841 gacgccctgc gcgtggttac ccgcagaagc agtgatcacg ccgtgcgctt tttgttcttc
    27901 cgtcaggccc gccatcatgg cgtatgcgcc gcgcagctta aagctgtgca ctggctggcg
    27961 atcttcgcgc ttcaccagaa tgacgttatc aagacgcgac gacagttttt ccattttttg
    28021 tagcggcgta acctgcgccg cctcgtaaac cggcgcgcgc agcactgctc ttaaatattc
    28081 ggcaccttcc ggagcaccgg acaggggttg cgagtcagcc attattaacc ccccagtttc
    28141 gatttatcgc gcaccgcacc tttgtcggcg ctggttgcca ggctggcgta ggcgcgcagg
    28201 gcaaaggaaa cctgacgttc acggtttttc ggcgtccagg ctttgtcacc ccgagcttcc
    28261 tgcgcttcac gacgcgccgc cagttcggca tcgcttacct gtaactgaat gccacggttc
    28321 gggatgtcga tagcgatcag gtcaccatct tcaatcagac caatgctgcc gccgcttgcc
    28381 gcttccggtg agacgtggcc gatggaaaga ccagaggtgc cgccagagaa acgaccgtcg
    28441 gtgatcagcg cacaggcttt accgagaccc attgatttca ggaagctggt tgggtagagc
    28501 atttcctgca tgccagggcc gcctttcggg ccttcatagc ggatcaccac cacatcgccc
    28561 gcgacaactt tgccaccgag gatcgcttct accgcgtcgt cctggctttc gtacactttc
    28621 gccgggccgg tgaatttgag gatgctgtca tcgacgcccg cggttttcac gatgcagccg
    28681 ttttcagcaa aattaccgta gagcaccgcc aggccgccgt ctttgctgta ggcgtgttcc
    28741 agcgagcgga tacagccgtt tgcgcgatcg tcatcgagag aatcccaacg gcaatcctgc
    28801 gagaatgcct gtgtagtacg aatgcccgca gggcctgcgc ggaacatatt ttttaccgcg
    28861 tcatcctggg tcagcataac gtcgtattgt tccagagttt gcggcaacgt caggccaagt
    28921 acgtttttca catcacggtt cagtaacccc gcgcgatcca gttcaccgag aataccgata
    28981 acaccaccag cacggtgaac atcttccata tggtattttt gggtgctcgg cgcaactttg
    29041 cataactgcg ggactttacg cgaaagtttg tcgatatcac tcatggtgaa gtcgatttcc
    29101 gcttcctgcg ccgccgccag caggtgaagt accgtgttgg tagagccgcc catcgcgata
    29161 tccagcgtca tggcattttc aaacgccgct ttgctagcaa tattgcgcgg cagtgcactt
    29221 tcgtcgtttt gctcgtagta acgtttggtc aattcaacaa tgcgtttacc ggcattgagg
    29281 aacaactgct tacggtccgc gtgggttgcc agcagcgagc cgttgcccgg ctgcgacagg
    29341 cccagcgctt cggtcaggca gttcattgag ttagcggtaa acatcccgga gcaggagccg
    29401 caggtcggac acgcggaacg ttcaacctga tcgctctggg agtcagaaac tttcgggtct
    29461 gcgccctgga tcatcgcatc aaccagatcg agtttgatta tctgatcgga cagtttggtt
    29521 ttcccggcct ccatcgggcc gccggaaaca aagatcaccg gaatattcag gcgcagggaa
    29581 gccatcagca tccccggggt gattttgtcg cagttagaga tgcagaccat ggcgtcggcg
    29641 cagtgggcgt tgaccatata ctcaacggaa tcagcgatca gttcgcgaga tggaagtgaa
    29701 taaagcatcc ccccgtggcc catggcaatc ccatcatcca ccgcaatggt gttgaactct
    29761 ttggcaacgc cgccagccgc ttcaatttgt tcggcgacca gtttaccgag atcgcgcaga
    29821 tggacgtgac ccggtacaaa ttgggtgaac gagttcacaa ccgcgataat cggcttgcca
    29881 aaatcggcgt cggtcattcc ggtggcgcgc cacagcgcac gagcacccgc catattacga
    29941 ccatgagtgg tggtggcgga acggtactta ggcatacttt atttactccc agtgtctgtc
    30001 tcgtaaatgg gacggtgcgt gccgtcccat tttttgtatt tattgattaa cttgatctaa
    30061 ccagccccat ttatcttcgg tttcgccagt gaagaggccg aagaaggctt gctgaatgcg
    30121 tttggtaacc gggccacaac ggccttcgcc aatctgaata ccgtctacgc tgcgcactgg
    30181 cgtgatttct gccgccgtac cggacataaa gacttcatcc gccaagtaca gggattcgcg
    30241 cgacagcacc tgctcacgca cttcaattcc cagctctttc gccagtttga tgatggcatc
    30301 acgggtaata cccggcagcg cggaggaggt gaacggtggg gtgaacagca caccatcttt
    30361 cacttcaaac aggttttcgc ctgcgccttc agagatataa ccgttcacat ccagcgcgat
    30421 accttcctga taaccgtggc ggcgcgcttc gctacccacc agcagggaag agagatagtt
    30481 accaccggct tttgccgcag ttgggatggt gtttggtgct gcgcggttcc aggaggaaac
    30541 catcgcatcg atcccctgct ccagcgcttc tgcgcccaga tacgctcccc acgggaaagc
    30601 ggcgataatc acatcggttg agtatcccgc tggcgggttt actcccatgc caacatcacc
    30661 gacgaagatc agcggacgga tataggcgct ggtgagattg tttttgcgga tcacgtcacg
    30721 acaagcttcc atcagctcat caatgctctg tgaaaccggg aagcgataga ttttggcgga
    30781 gtcatgcaga cgctgcatat gctcacgatg gcggaataca accggtcctt tgtgtgagtc
    30841 gtagcaacgg atgccttcaa aaaccgaggt gccatagtgc agcgcgtgcg acatcacatg
    30901 caccttcgcg tcttcccagc gaaccatctc cccattgaac caaatgtaat cagctttctt
    30961 cgtggtcatt tttatattcc ttttgcgctc aggcgcggat ttgttgtgat gtggttgtgc
    31021 tctggcagat ggcaacgtgt gcgacgtcca ccagtttatt taactgacta aacagtaagt
    31081 cgaccgaccg tgggctggca acggtcaatt cgatatttat attttgtgca tcgctggcgg
    31141 cagccatatt cattgagcag acgtggaaac cacgatgacg caccacgcgt aaaacacgtt
    31201 ctaaggtttc cggattgaag cgagccgata cattgacctg atgttgcatc atgataattt
    31261 ctccaacatt tctgaattac tggcacctgg cggcaccagc ggccagacgt tctcaagttc
    31321 gtcgattgag acatgaagca ggtatggccc atcactgttc agcatggtgt cgagtgccgc
    31381 ttcaacctgg tctttacggg taatgtgttg gccagggatg ccgaaggcgc tggctaacat
    31441 gaggaaatcg gggttatcgg taagggtggt ttcgctgtat cgttcctgaa aaaacagttg
    31501 ctgccattgt cgaaccatcc ctaaccgttg gttatcgagt aagacgattt tcaacggtaa
    31561 ctgcttgcgt tttacggtgc ccagctcttg cacattcatc atgaaagagc cgtcaccgga
    31621 gatacagaca acggtatcgt tcggtcgcgc gacttgtgcg ccaactgccg ccggtaaacc
    31681 aaaacccatg gtacctaagc cgctggaggt gatgaaattt tccgggcgag tgtgggcgat
    31741 gtgctgcgca gcccacatct ggtgctgccc cacatctgtg gtcacgacgc aatccgcagg
    31801 tttacgatcc gacagttgtt ttaacaacaa cggagcgtag atagcgtcac cgggatggtc
    31861 gtaacgccag gaatgttcat cacgcagctg cgcgcagtgt tgctgccagt cattgatatt
    31921 taacggctgc tgtaatgctg gtaacagagc atttaaatca ccttgtaatg ccacatgtgc
    31981 ctgacgcagc ttgttcattt ctgccgggtc gatatccata tggataacac tggcgtgtgg
    32041 tgcgaaggtg ttcagtttgc cggtcacccg gtcatcaaaa cgtgcaccca cggcgatcag
    32101 caggtcacac tcctgcaccg cgaagtttgc cgctttggtt ccgtgcatcc ccagcatgcc
    32161 cagatagtac ggataatctg cttctactgc gcccagccct ttcagcgtac aggtggcagg
    32221 catttttgtg gcagcgagaa attcacgcaa agccggaact gcctgcgcca tccccacgcc
    32281 accgccaacg tacagcatcg gtttttgcgc ttttgccagc atctggcgcg cttgctcgac
    32341 ttcggcatgt gggaaagtca cttcgttttc aacggtggtg aaccacggtt ccagatcgcc
    32401 gctggctaac tggatatctt ttgggatatc gaccagaacc ggaccaggac gacctgagct
    32461 ggcaacgtcg aatgcttcag ccatgatgcg cggcaactct tccagagact gcaccagaaa
    32521 gctgtgcttg gtacaggcta acgacaatcc caggacatcc acttcctgaa atgcgtcggt
    32581 gccgataaac ggtgcggaca cctgaccagt gatggcaacg acagggatgg aatctaacag
    32641 tgcgtccgca agcccggtta tcaggttggt tgcgcccgga ccagacgtgg cgatacatac
    32701 gccagtttta ccggtagcac gggcataacc gatagccgct attgccgcac cctgctcatg
    32761 tcggcacagc aagtgctcca cgccgccgtc atacaatgca tcgtaaaccg gcataattgc
    32821 gccacccgga taaccgaaaa cggtgttcac accctgtgcc cgcaacgcat gtaccaccca
    32881 ctgtgcgcca ttcatagtta gttccccgtc ctgaatcttg agaaacagaa ttttgtgctg
    32941 ttattcattg tctgctcctc ggttatgttt ttaaggtcaa aaaaaccccc ggacctttcg
    33001 gtgcgggggt cttagttcgt taaggcttga tctctaagcc tttcctcgtc caagtgcagc
    33061 cccgcacggt gggataataa tcaccaccac gctaatcacg accaggctaa tcactcgtag
    33121 aagggctgtc attttgtctt ttcttgcatc ttgttcgaag gaatgcctaa agagttacca
    33181 tagattttgc gaatagcaca agatattttt gggcctattt ttatgatggc tttaataatt
    33241 tcaatagaaa ttgttgtttt ttaaaaagaa aatagtgagt gaaaaaattc aatttcagta
    33301 taaatgttgt tcccttcctc gtagttcatc ctttgtccgc tgcatttttg cgagcatcat
    33361 tccaccgcca gaacaactca acttcacttg cacaaataaa caggaatcag ccactaaaat
    33421 tcacgaatta gcgggctaca gcagcgatat cttatgcaca taatggtgac tcaaggaggg
    33481 tttatgtcac tgtcaattgt tcatacccgc gcagccctgg aagtaaatgc gcccccaatc
    33541 actgttgagg tacatatcag taaaggtcta cccggcttaa cgatggtggg cttaacagaa
    33601 acaacggtga aagaagctcg cgatcgcgtg cgcagcgcca ttatcaatag cggatatgaa
    33661 tatccggcga aaaaaatcac catcaacctt gctccagccg atctgccaaa agaaggggga
    33721 cgatatgatt tacctatcgc cattgcgttg ctggcggcct cagaacagct tacagccaat
    33781 aagttagatg aatatgaatt agtcggagaa ctagcgctta caggcgctct gcgtggcgtt
    33841 cccggtgcaa tctccagtgc aactgaagct attaagtcgg gcagaaaaat tatcgtcgcg
    33901 aaagataacg aagatgaagt ggggctaatt aacggtgaag gatgcctgat agccgatcat
    33961 ctgcaggctg tctgtgcgtt tctggaaggt aagaacactc tcgaacgccc gaaaccaact
    34021 gatgcagtat cccgggcgct acaacatgat ctcagtgatg ttgtcggtca ggagcaagga
    34081 aaacgaggac tggaaattac cgccgctggt gggcacaacc ttttactgat tgggccgccg
    34141 ggaacaggta aaacaatgct cgccagccgt attaatggcc tgttgccaga tttaagcaat
    34201 gaagaggcac tggagagcgc tgcgatatta agtctggtaa atgctgaatc agtacaaaaa
    34261 caatggcggc agcgcccgtt ccgctcacct catcacagtg cgtcgttaac tgcgatggtg
    34321 ggcggtggtg caattccagg gccaggtgaa atttcgttgg cgcataacgg cgtgcttttt
    34381 cttgatgagc tacctgaatt tgaacggcgt acactggatg ccttgcgaga gccgattgaa
    34441 tccgggcaga tccatctttc acgcacacga gcaaaaataa cctatccagc ccgtttccag
    34501 cttgttgcgg cgatgaatcc cagccctacc ggacattatc agggaaacca taaccgctgc
    34561 acgccagaac agacattacg ttatctcaac cggctctcgg ggccctttct cgaccgcttc
    34621 gatctctcac tggagatccc attaccaccc cccggcattt tgagtaaaac ggtagtgccg
    34681 ggagaaagca gcgccaccgt taaacaacgc gtaatggccg ccagagagcg ccaatttaag
    34741 cggcagaata aactgaatgc ctggctggat agtccggaaa tacgccaatt ctgcaaactt
    34801 gagagcgaag atgcgatgtg gttggaagaa acgctgatcc atctggggtt atcgattcgt
    34861 gcctggcagc ggttactgaa agttgcacga accattgctg atattgatca gtctgacatt
    34921 atcacacgtc agcatttgca ggaggcagtt agctatcgtg cgattgaccg tttgctcatc
    34981 catctgcaaa aactactgac ataaaaaaag ggcatttcgc cctttttatt aatcgtcaga
    35041 atcggtgtag tcttcagcac cttcaacctg cggtttaccg ccggaaaggg tgtgaaaacg
    35101 ttttggacgc ttaatacgcg tcatatactt ggaccacacg cgttctgctt ctgtcactgg
    35161 ctcacgttcg ccacggcata ctgctacgaa gagtttctct tcctcggtaa ccggctcgcg
    35221 tttgccaaga tccaactcat tgaaggcata accatgacgc tcaagcagtt gtgcctcttt
    35281 gatggtgaaa tcaccatgac gagagaatcc acgtggataa tgtttattgt cgaaatatcg
    35341 attagtcgtc gtaaagcttt ccgccatcct gcacgctcct aattctttga ccgagctagt
    35401 tatggcgcgg agtattagtt acgcttgaca gagtgtaaaa caaaacattt aaatcataac
    35461 gacaaataat tttgcggaga gcactgtgga tacggaattg ttaaaaactt tcctggaagt
    35521 tagccgaacg cgtcactttg gtcgagcggc tgaatcgctc tatctgaccc agtcagcagt
    35581 gagctttcga atcagacaac tggaaaatca actgggtgtg aaccttttca cccgccacag
    35641 aaacaatatc cgtttaaccg ctgccggtga aaaactactg ccttatgcag aaacgctcat
    35701 gagcacctgg caggccgccc gcaaggaggt ggcgcatacc tcacgacata acgagttttc
    35761 tatcggtgcc agcgcctcgt tgtgggaatg tatgcttaat cagtggttgg gacgcttgta
    35821 tcaaaatcag gatgcccata caggcttaca gttcgaagcg cgaattgccc aacggcagtc
    35881 tctggtaaaa cagctgcatg aacgccagct tgatcttctt attaccactg aagcgcccaa
    35941 aatggacgaa tttagtagtc agttgctggg gtatttcact ttagcgcttt ataccagtgc
    36001 cccttctaaa ctaaagggag atcttaacta tctgcgactg gagtgggggc cagattttca
    36061 acagcatgag gcaggtttga gcggtgctga cgaagttccc attctgacaa ccagttctgc
    36121 tgaactggca cagcaacaga ttgcgatgct taatggttgc acctggctac ccgtcagctg
    36181 ggcgcgtaaa aaaggcagcc tgcataccgt tgtcgatagc acaacacttt cacggccgct
    36241 ttatgccata tggctgcaaa atagcgataa aaatgcgttg attcgcgatc ttttgaaaat
    36301 taacgtgctg gatgaagtgt attaatctga atggctggca aggatgccgg tagaaggatt
    36361 tacttcggag aggattattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc
    36421 tggcaggggc ggagagactc gaactcccaa cacccggttt tggagaccgg tgctctacca
    36481 attgaactac gcccctaatt agggtggcgg aacggacggg actcgaaccc gcgaccccct
    36541 gcgtgacagg caggtattct aaccgactga actaccgctc caccgaattc ttttacaacc
    36601 accggtttta tgaccggctt ac
//
LOCUS       DAETPZ010000032        36607 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.032, whole genome shotgun sequence.
ACCESSION   DAETPZ010000032 DAETPZ010000000
VERSION     DAETPZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.032"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            27..2294
                     /gene="adiA"
                     /locus_tag="K0W59_001838"
     CDS             27..2294
                     /gene="adiA"
                     /locus_tag="K0W59_001838"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HBI3022897.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     INEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVIHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            2619..3380
                     /gene="adiY"
                     /locus_tag="K0W59_001839"
     CDS             2619..3380
                     /gene="adiY"
                     /locus_tag="K0W59_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HBI3022898.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            3517..4854
                     /gene="adiC"
                     /locus_tag="K0W59_001840"
     CDS             3517..4854
                     /gene="adiC"
                     /locus_tag="K0W59_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HBI3022899.1"
                     /translation="MSSDADAHKVGLIPVILMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            4958..6601
                     /gene="eptA"
                     /locus_tag="K0W59_001841"
     CDS             4958..6601
                     /gene="eptA"
                     /locus_tag="K0W59_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HBI3022900.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRTLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            6598..7266
                     /gene="basR"
                     /locus_tag="K0W59_001842"
     CDS             6598..7266
                     /gene="basR"
                     /locus_tag="K0W59_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HBI3022901.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            7267..8367
                     /gene="pmrB"
                     /locus_tag="K0W59_001843"
     CDS             7267..8367
                     /gene="pmrB"
                     /locus_tag="K0W59_001843"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HBI3022902.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(8343..8432)
                     /gene="pmrR"
                     /locus_tag="K0W59_001844"
     CDS             complement(8343..8432)
                     /gene="pmrR"
                     /locus_tag="K0W59_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HBI3022903.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(8544..10046)
                     /gene="proP"
                     /locus_tag="K0W59_001845"
     CDS             complement(8544..10046)
                     /gene="proP"
                     /locus_tag="K0W59_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HBI3022904.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(10310..11188)
                     /locus_tag="K0W59_001846"
     CDS             complement(10310..11188)
                     /locus_tag="K0W59_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HBI3022905.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(11185..13413)
                     /gene="crfC"
                     /locus_tag="K0W59_001847"
     CDS             complement(11185..13413)
                     /gene="crfC"
                     /locus_tag="K0W59_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HBI3022906.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            13815..14150
                     /locus_tag="K0W59_001848"
     CDS             13815..14150
                     /locus_tag="K0W59_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HBI3022907.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            14308..14751
                     /gene="yjdN"
                     /locus_tag="K0W59_001849"
     CDS             14308..14751
                     /gene="yjdN"
                     /locus_tag="K0W59_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HBI3022908.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            14884..15672
                     /gene="phnC"
                     /locus_tag="K0W59_001850"
     CDS             14884..15672
                     /gene="phnC"
                     /locus_tag="K0W59_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HBI3022909.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            15697..16713
                     /gene="phnD"
                     /locus_tag="K0W59_001851"
     CDS             15697..16713
                     /gene="phnD"
                     /locus_tag="K0W59_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI3022910.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            16819..17598
                     /gene="phnE"
                     /locus_tag="K0W59_001852"
     CDS             16819..17598
                     /gene="phnE"
                     /locus_tag="K0W59_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HBI3022911.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAEEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            17619..18344
                     /gene="phnF"
                     /locus_tag="K0W59_001853"
     CDS             17619..18344
                     /gene="phnF"
                     /locus_tag="K0W59_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HBI3022912.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVAEALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            18345..18797
                     /gene="phnG"
                     /locus_tag="K0W59_001854"
     CDS             18345..18797
                     /gene="phnG"
                     /locus_tag="K0W59_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBI3022913.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHTERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            18794..19378
                     /gene="phnH"
                     /locus_tag="K0W59_001855"
     CDS             18794..19378
                     /gene="phnH"
                     /locus_tag="K0W59_001855"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBI3022914.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAISEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDDGIS
                     SKQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            19378..20442
                     /gene="phnI"
                     /locus_tag="K0W59_001856"
     CDS             19378..20442
                     /gene="phnI"
                     /locus_tag="K0W59_001856"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HBI3022915.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGVADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLVHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            20435..21280
                     /gene="phnJ"
                     /locus_tag="K0W59_001857"
     CDS             20435..21280
                     /gene="phnJ"
                     /locus_tag="K0W59_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBI3022916.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHVLEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            21277..22035
                     /gene="phnK"
                     /locus_tag="K0W59_001858"
     CDS             21277..22035
                     /gene="phnK"
                     /locus_tag="K0W59_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HBI3022917.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            22146..22826
                     /gene="phnL"
                     /locus_tag="K0W59_001859"
     CDS             22146..22826
                     /gene="phnL"
                     /locus_tag="K0W59_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HBI3022918.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            22823..23959
                     /gene="phnM"
                     /locus_tag="K0W59_001860"
     CDS             22823..23959
                     /gene="phnM"
                     /locus_tag="K0W59_001860"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBI3022919.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLLLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHLLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     SGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTQN
                     PAQALNLQDRGVIGEGKRADLVLAHRKDNRIHIDHVWRQGKRVF"
     gene            23959..24516
                     /gene="phnN"
                     /locus_tag="K0W59_001861"
     CDS             23959..24516
                     /gene="phnN"
                     /locus_tag="K0W59_001861"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HBI3022920.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAEIRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANSLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQALARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            24503..24937
                     /gene="phnO"
                     /locus_tag="K0W59_001862"
     CDS             24503..24937
                     /gene="phnO"
                     /locus_tag="K0W59_001862"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HBI3022921.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVSMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTALSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            24939..25697
                     /gene="phnP"
                     /locus_tag="K0W59_001863"
                     /pseudo
     CDS             24939..25697
                     /gene="phnP"
                     /locus_tag="K0W59_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
     gene            complement(25844..26173)
                     /locus_tag="K0W59_001864"
     CDS             complement(25844..26173)
                     /locus_tag="K0W59_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022922.1"
                     /translation="MKPYSTLFLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNSDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRCQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(26242..26874)
                     /locus_tag="K0W59_001865"
     CDS             complement(26242..26874)
                     /locus_tag="K0W59_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBI3022923.1"
                     /translation="MIVMPTTYSPMTVARSFRVVEEIELSGRLLQVIYDNQTPRVAII
                     EADIEVFAGVPRHRVLAALDLQRKKEFGSDIIAVERWWEDSDLVQVEGICVSPGERDK
                     GLATRLYEALILQCGITLISDFDQYDGGKMLWQKIARESDQISVFVLDTEQGAFWPYD
                     GTKVIYDGGCIPEERIWSISPDQKCHGIVLVAENKQRANQLMEESAPYRI"
     gene            complement(26955..29170)
                     /locus_tag="K0W59_001866"
                     /pseudo
     CDS             complement(26955..29170)
                     /locus_tag="K0W59_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
     gene            29284..30804
                     /locus_tag="K0W59_001867"
     CDS             29284..30804
                     /locus_tag="K0W59_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBI3022924.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLTVAENI
                     FLGSELSHGGLVQRKEMLVQAQRVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSCL
                     QVLSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAVENITMATLERDASWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPTRGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVHVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            30801..31775
                     /locus_tag="K0W59_001868"
     CDS             30801..31775
                     /locus_tag="K0W59_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBI3022925.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVLCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSTSRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            31806..32747
                     /locus_tag="K0W59_001869"
     CDS             31806..32747
                     /locus_tag="K0W59_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBI3022926.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKDLKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNKNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     ISYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            32802..33485
                     /locus_tag="K0W59_001870"
     CDS             32802..33485
                     /locus_tag="K0W59_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HBI3022927.1"
                     /translation="MKRSAINNILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     VVFDLKLGWDVTAFGGNNFAAQGLMLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTVDTDVTVVIDGCRQTHVAGSQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFL"
     gene            33500..34360
                     /locus_tag="K0W59_001871"
     CDS             33500..34360
                     /locus_tag="K0W59_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HBI3022928.1"
                     /translation="MPLISLAEGLAHAREHHYALGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARQAIPVVLNLDHGLHFESVAQALRLGFSSVM
                     FDGSTLGYEENIRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGIRDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            34357..35358
                     /locus_tag="K0W59_001872"
     CDS             34357..35358
                     /locus_tag="K0W59_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HBI3022929.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLLLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHCPEGAWGETPEQCGVWIPSQKLEQREIIGS
                     VGAGDVFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(35355..36061)
                     /locus_tag="K0W59_001873"
                     /pseudo
     CDS             complement(35355..36061)
                     /locus_tag="K0W59_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            36352..>36607
                     /locus_tag="K0W59_001874"
     CDS             36352..>36607
                     /locus_tag="K0W59_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022930.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYY"
BASE COUNT         8526 a        10223 c         9724 g         8134 t
ORIGIN      
        1 attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc gagtttctcc
       61 atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta agccagcaaa
      121 atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc tcttcaaacg
      181 aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa catcaaaacg
      241 tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc ttcctgttgg
      301 gcgatcggga aaaagccctc gccgcaatgg accgggatct gctggagctt gtcgatgaat
      361 tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgccgtt gccgcgatga
      421 cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa tatagtgaca
      481 tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt accaaaacac
      541 ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc gacatgggca
      601 tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc gaaagcgaaa
      661 aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc ggtacttccg
      721 gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg gtcgttgacc
      781 gtaactgcca taaatccatc gaacaaggtt tgatgctgac aggcgcgaaa ccggtctata
      841 tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag gaaatgcaac
      901 ctgaaacctt gcaaaagaaa atcagtgaaa gcccgctgac caaagacaaa gccgggcaaa
      961 aaccgtctta ctgcgtggta accaactgca cctatgacgg cgtgtgttat aacgctaaag
     1021 aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa gcctggtacg
     1081 gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc gaacctggcg
     1141 atcacaacgg tcctaccgtt ttcgccaccc actccatcca caaactgctg aatgcgctgt
     1201 cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc tcccgcttca
     1261 accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc gcatccaacg
     1321 acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacacag gaagtgatta
     1381 acgaagcggt tgatttccgt caggcgatgg cgcggctata taaagagttc accgctgacg
     1441 gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa accggcaaaa
     1501 cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac tgctgggtaa
     1561 tacatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg agtatgctcg
     1621 acccaattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa ctggaagaaa
     1681 ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt gtgcctaccc
     1741 gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt gggaaatggg
     1801 ggactctggt taacaccctt tgctccttca aacgccacta tgacgccaac acaccgctgg
     1861 cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac atggggattc
     1921 acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc gcacggttga
     1981 acgaagccta ttccggcctg ccggtggcgg aaatcacccc gcgtgaagcg tacaacgcga
     2041 ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc atcgcggcaa
     2101 actcagttat cccgtatccg ccaggaatcc cgatgctgct gtctggtgaa aacttcggcg
     2161 ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac caccatttcc
     2221 ctggatttga acacgaaact gaagggactg aaattattga cggtatttac cacgttatgt
     2281 gcgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac attacgccgc
     2341 ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca aacaaaaatg
     2401 tcatactttt tgcgcggccc caccccgcgc ttttttttgc ctgttattta tcctgtaaaa
     2461 aaatatgtac atgagaaatt actataaaaa tttgtaatat tagtaaaact cgttattttt
     2521 atgcatgttt atattcatca tacaattata taaccatttc ccggtatcgc tttgctttag
     2581 cgagaactgg tcttttattc gcactcagga gtacatgtat gaggatttgc agcgaccaac
     2641 cttgtattgt tttattgact gaaaaagatg tctggataag ggtgaatggg aaagaaccta
     2701 ttagccttaa agctaaccat atggcgttat taaattgtga aaataatatt atcgacgtct
     2761 cctctcttaa caacactttg gttgctcata ttagtcacga catcatcaaa gattacctcc
     2821 ggtttctgaa taaagatctc tcgcaaatac cagtatggca acgtagcgct acgcccatac
     2881 tcaccctgcc atgcctgacg ccagacgtct ttcgcgttgc cgcgcaacac agcatgatgc
     2941 ccgcagaaac tgagtcagaa aaggaacgaa cacgcgcatt attattcacg gtgctatccc
     3001 gttttctcga cagtaaaaaa ttcctttcat taatgatgta tatgttacgt aattgtgtaa
     3061 gtgacagcgt ttatcaaatt attgaaagcg atattcacaa agactggaat cttagtatgg
     3121 tagccagttg tttatgtctt agcccaagtc tgttaaagaa aaagttgaaa agcgaaaaca
     3181 ccagttatag ccaaataatc accacctgcc gcatgcgtta tgccgtaaat gaattaatga
     3241 tggacggtaa aaatatctct caggtatcac agtcctgcgg ctacaacagt acgtcgtact
     3301 ttatttctgt ctttaaagac ttctacggta tgacgccgct gcattatgtt agtcagcaca
     3361 gagaacgcac tgtcgcctga tttttaacct taacgaagag ctatattaat aacggcatca
     3421 gcgataaccc ggtcgataat aattcaacta tcgaatgcag gcgtatgata tgacgtaatt
     3481 tattgtcacg aagctcgcct tcgcaggagt ttaattatgt cttcggatgc tgatgctcac
     3541 aaagtgggct taatccccgt catcctgatg gtgtcgggga atattatggg gtcaggtgtt
     3601 tttctgttac ctgcaaacct ggcctccact ggcgggattg ccatttatgg atggttggtg
     3661 acgattatcg gtgcgctggg gctctcgatg gtatacgcca aaatgtcgtt cctcgaccca
     3721 agtcctggtg gttcttacgc ttacgcccgc cgctgctttg gcccgtttct cggttatcaa
     3781 accaacgtcc tctactggct ggcctgctgg atcggcaata tcgccatggt ggtcattggc
     3841 gtaggatatt taagttactt cttcccgatt ctgaaagatc cattggtatt aaccatcacc
     3901 tgcgtcgtgg tgctgtggat cttcgtcctg ctgaacattg tcggtccgaa aatgatcacc
     3961 cgcgtgcagg ccgttgccac tgtgctggcg ctgatcccca tcgtcgggat tgccgtattt
     4021 ggctggttct ggttccgtgg tgaaacctat atggcggcat ggaacgtcag cggcctgggc
     4081 accttcggtg cgattcaaag tacccttaac gttacgctgt ggtcgttcat cggtgtggaa
     4141 agtgcctccg ttgccgcagg tgtggtgaaa aacccgaaac gcaatgtccc tatcgccacc
     4201 attggtgggg tattgattgc cgccgtttgc tatgtgctct ccaccaccgc gattatgggg
     4261 atgatcccta atgccgcact gcgcgtttct gcctcgccat tcggtgatgc cgcacggatg
     4321 gcgttgggtg acaccgctgg ggccattgtt tccttctgcg cagctgcggg ttgcttaggt
     4381 tcactgggcg gctggacgtt gctggcgggt caaacggcga aagccgctgc cgatgacgga
     4441 ctgttcccac cgatttttgc ccgtgtaaat aaagcgggta cgccagtggc ggggctgatt
     4501 atcgtcggta ttttgatgac catcttccag ctcagcagca tttcaccaaa cgcgaccaaa
     4561 gagttcggtc tggtttcttc cgtctcggtc atctttacac tggtgccata tctttacacc
     4621 tgtgcggcgt tactgctgct cggccacggt cactttggta aagcacgccc ggcatatctg
     4681 gctgttacta ccattgcctt cctctactgc atttgggccg tggtggggtc cggggcgaaa
     4741 gaggttatgt ggtcatttgt caccctgatg gtcatcaccg ccatgtatgc gctgaattac
     4801 aaccggctac ataaaaaccc gtatccctta gatgcaccaa taagcaaaga ttaattcccc
     4861 ttaatccagc aaacataaaa gccaacctta agaacttaag gttggcttaa ttttgctttg
     4921 cgagcatatg cgcactttgt tcgatggaaa caccgtgatg ttgaagcgcc tactaaaaag
     4981 accctctttg aatttactcg cctggctatt gttggccgct ttttatatct ctatctgcct
     5041 gaatattgcc ttttttaaac aggtgttgca ggcgctgccg ctggattcgc tgcataacgt
     5101 actggttttc ttgtcgatgc cggtcgtcgc tttcagcgtg attaatattg tcctgacact
     5161 aagctctttc ttatggctta atcgaacact ggcctgcctg tttattctgg ttggcgcggc
     5221 tgcacaatat ttcataatga cttacggcat cgtcatcgac cgctcgatga ttgccaatat
     5281 tattgatacc actccagcag aaagttatgc gctgatgaca ccgcaaatgt tattaacgct
     5341 gggattcagc ggcgtgcttg ctgcgctgat tgcctgctgg ctaaaaatca aacctgccac
     5401 ctcgcgtctg cgcagtgttc ttttccgtgg agccaatatt ctggtttctg tactgctgat
     5461 tttgctggtc gccgcactgt tttataaaga ctacgcctcg ttgttccgca ataacaaaga
     5521 gctggtgaaa tccttaagcc cctctaacag cattgttgcc agctggtcat ggtactccca
     5581 tcagcgactg gcaaatctgc cgctggtgcg aattggtgaa gacgcgcacc gcaacccgtt
     5641 aatgcagaac gaaaaacgta aaaatttgac catcctgatt gtcggcgaaa cctcgcgggc
     5701 ggagaacttc tccctcaacg gctacccgcg tgaaactaac ccgcggctgg cgaaagataa
     5761 cgtggtctat ttccctaata ccgcatcttg cggcacggca acggcagttt cagtaccgtg
     5821 catgttctcg gatatgccgc gtgagcacta caaagaagag ctggcacagc accaggaagg
     5881 cgtgctggat atcattcagc gagcgggcat caacgtgctg tggaatgaca acgatggcgg
     5941 ctgtaaaggt gcctgcgacc gcgtgcctca ccagaacgtc accgcgctga atctgcctga
     6001 tcagtgcatc aacggcgaat gctatgacga agtgctgttc cacgggctgg aagagtacat
     6061 caataacctg cagggtgatg gcgtgattgt cttacacacc atcggcagcc acggtccgac
     6121 ctattacaac cgctatccgc cgcagttcag gaaatttacc ccaacctgcg acaccaatga
     6181 gatccagacc tgtaccaaag agcaactggt gaacacttac gacaacacgc tggtttacgt
     6241 cgactatatt gttgataaag cgattaatct gcttaaagaa catcaggata aatttaccac
     6301 cagcctggtt tatctttctg accacggtga atcgttaggt gaaaatggca tctatctgca
     6361 cggtctgcct tatgccatcg ccccggatag ccaaaaacag gtgccaatgc tgctgtggct
     6421 gtcggaggat tatcaaaagc ggtatcaggt tgaccagaac tgcctgcaaa aacaggcgca
     6481 aacgcaacac tattcacaag acaatttatt ctccacgcta ttgggattaa ctggcgttga
     6541 gacgaagtat taccaggccg cggatgatat tctgcaaact tgcaggagag tgagtaaatg
     6601 aaaattctga ttgttgaaga cgatacgctg ttattgcagg gactgattct ggcggcgcaa
     6661 accgaaggct acgcgtgcga tggcgtgaca accgcgcgga tggcggaaca aagccttgag
     6721 gacggtcatt acagcctggt ggtactggat ttagggttac ccgacgaaga tggactgcat
     6781 tttctcgccc gtatccggca gaaaaaatac accctgccgg tactgatcct caccgctcgc
     6841 gatacgctga ccgacaaaat cgccgggctg gatgtcggtg ccgatgacta tctggtgaag
     6901 ccttttgcgc tggaagagtt acacgcccgt atccgcgccc tgctacgacg ccataataat
     6961 cagggcgaaa gtgagctgat tgttggcaat ctgacgctga atatgggtcg ccgtcaggta
     7021 tggatgggcg gtgaagagtt gattctgacg cccaaagaat atgctctgct gtcacggtta
     7081 atgctcaaag caggcagtcc ggtacatcgg gaaattctct acaacgacat ctataactgg
     7141 gacaatgaac cctcgaccaa caccctggaa gtgcatatcc acaatctgcg cgacaaagtg
     7201 ggcaaagccc gtatccgcac cgtgcgcggc tttggctata tgctggtcgc gaatgaggaa
     7261 aactaattga atctgatgcg ttttctgcgc cgaccaatat cgctgcgcca acggctgata
     7321 ttgaccatcg gggccatttt gttggtgttt gagctgatca gcgtcttctg gctatggcat
     7381 gaaagtaccg agcagattca actgtttgag caggcgctac gcgacaatcg caacaacgat
     7441 cgccacatca tgcgtgagat ccgcgaggcg gtcgccagcc tgattgtccc cggcgtcttt
     7501 atggtcagcc tgacgctatt tatctgctat caggcggtac gccgcatcac ccgcccgctg
     7561 gcggagctgc aaaaagagct ggaagcgcgc accgccgaca acttaacgcc cattgccatt
     7621 cacagcgcca ccctcgaaat cgaagcggtg gtttcggcgt tgaacgatct ggtcagtcgc
     7681 ctgaccagca cgctggataa cgaaaggttg tttaccgctg acgtcgcgca cgaactgcga
     7741 acgccactgg cgggggtgcg tttgcatctg gaactgctgg cgaaaacgca tcacattgat
     7801 gtagcaccgt tagtggcacg gcttgatcag atgatggaga gcgtctccca gctgctgcaa
     7861 ctggcgcgtg ccggacagtc attttcttcc ggtaattatc aacatgtaaa actgctggaa
     7921 gatgtcattc tcccctcgta tgacgaactc agtaccatgc tcgaccagcg acagcaaacc
     7981 ctgctattgc cagagagcgc cgccgacatc accgttcagg gtgatgcaac cctgctgcgg
     8041 atgttattgc gaaacctggt agaaaacgcc catcgttaca gcccgcaagg cagcaacatt
     8101 atgattaagc tgcaagaaga tggcggagcg gtcatggcag ttgaagatga aggaccaggt
     8161 attgatgaga gtaaatgcgg ggagttgagt aaagcgtttg tacgtatgga cagccgttat
     8221 ggcgggattg gtctggggtt aagtattgtc agccgcatta cacagttgca tcacgggcag
     8281 tttttcctgc aaaaccggca agagacttcc ggcacgcggg cctgggtacg gctgaagaaa
     8341 gatcagaacg tggcaaacca gatataaaga aagctgctga ccaccagcac gctgaacacg
     8401 gtagttaaac tttcataaac acggtttttc attgcactct cctccgttaa cctggaggag
     8461 agtatgcgcg tcagagatta agtgaacctt aagagttcaa ttggcctgat aagacagcgt
     8521 cacatcaggc catccgtttc agcttattca tcaattcgcg gatgttgctg caccaggcgg
     8581 gtacgtttcg cctgcaaatc ggcaatctcg tggtcaatat tatcgatttt ctgctcgata
     8641 ttatcgtaat gctcgacgag aatttccttc gcttcctgta tatctgacgc cgccggtgtc
     8701 gcacctttca acggacgatt tgccgtctct ttcatggtta cgccggtgat taaaccaatc
     8761 accgccacta ccatcaggta ataggcaggc atcatcagat tctgcgagct ttcgaccagc
     8821 caggccgcca gtgttggcgt cagaccggca accagcaccg aaatattaaa tgccgccgcc
     8881 agcgcgctgt aacggatatg cgtcgggaac atcgctggca aggtagaagc cataacgccc
     8941 ataaagcagt taaggatcac cgccagcatc agtaacccgg caaaaatcag gccgatgacg
     9001 ttactgttaa tcagaataaa cgccgggatc gccaacacaa acagtgcaac actaccaagt
     9061 agcacaaacg gacgacggcc aaaacggtca ctcagcaagc ccatcaccgg ctggacaaac
     9121 agcataccga tcataatggc gataataatc agcaccccgt ggtcttcgga gtaatgcagg
     9181 ttatgcgaca aataactcgg catataggtc agcaacatgt agtaagtcac gttggtggca
     9241 attaccagac caatacatgt caacaggctg cgccagtatt tagtggcaat ctctttaaac
     9301 gagactttcg ggccatcctg caaaccttcg cggtcgccct gttccagttt atcaacatgc
     9361 tgctggaacg ccggagtttc ttccagcgca tggcgcaggt aaagcccgat aatccctaac
     9421 ggcagagcaa taaagaacgg aatacgccag ccccagtcga ggaagttcgc ttcgccgaca
     9481 atggtcgaaa ttaacaccac cacgcccgca cccagcacaa acccggcaat agaaccgaag
     9541 tccagccagc tgcccataaa gccacgttta cggtcggggg agtattccgc aacaaatatc
     9601 gacgccccgg tatattcacc accgaccgag aaaccttgtg ccatcttaca gatcaacagc
     9661 agaatcggtg cccaaatacc aatcgtgtcg taggacggta ttaagccaat acagaacgta
     9721 ctgatcgaca taatcacaat agtgatagcg aggatcttct ggcgaccata tttatcgccc
     9781 aacataccaa agaagagtcc gccaagcggt cgaatcagaa agggaacgga gaaagtggca
     9841 agtgcagcaa ccatctgcac gctggggtca gcccccggga aaaaaacttt acctaatgcg
     9901 taagcaacaa aaccataaac accaaaatca aaccattcca ttgcattacc cagtgatgct
     9961 gcggtaatgg ctttacgcag tttaccgtca tcaataatgg tgacatcacg aagggtaatc
    10021 ggttttactt ttttcctttt cagcatagct ttcctcgcag agttgaccca tttccgcaaa
    10081 cgccagaaaa atcagcgcga cggccgcaaa aggggagcgg gaattgcagg atgcaatctc
    10141 tgtaacaagc gtagcgggtt tgcttacacc ctccggttaa ggaatgtaca atcaaaccag
    10201 ttgacgcctg aattgggcag ttaatgacct ttttacccta ccagtgttta aatttgtgat
    10261 caacttcaca catggatttc gtttaggact cattgatgta actgattatt tacgccaaat
    10321 ttacagagaa gatttcctcg accattgccc ccaccaaacg ctgcgctgaa cagagtcgtg
    10381 gcatccccag cgcgacagtt tgccagcgtt gggtcactga ccagcaaacg ggttgtttat
    10441 ctgccgtggt ccactttccc agccaactta aaaaatcagc atgatccccg cccgtcggaa
    10501 cggtcgaaga ggcaaaccac tgatggtaat agtgacgcgt ggtcggtgcc gcattgatac
    10561 tctctgccag atgttgtgtc gctttctcgc gcaaatcacc cagcaacaac accaccgctt
    10621 gcgggcttcc cagacggcag gcatcgccaa atgcacccca gcgcaactcc tccagaacga
    10681 taaaacagcg cccaggcaac gaccaggcgt tatacgcccc agcacgccag cgggtaaaaa
    10741 tttgctcgct ctgatcacct gcctgcacca gtaggcctcg ttgcgttaac gccttttcct
    10801 tccagcgaca acgggctgaa aaccactcat acaaggcggc aatttgctgg gggagctccg
    10861 ggaaagcaga gtgctggcgg tgatccattt tttgcagcag cgccagatgc tgcgcgatct
    10921 gcgtcagcgc cagatgaccg ggatccagca tcgacgccag tttttccacc attgtcagtc
    10981 gcagactaag cggcgcattt aacatgccgc cgttcgtcca tgtctgaagc tgcacacgcg
    11041 aaagaagttc ctgcatcagt cgttcacgaa actgctgctg atgggcttga ggatggcggg
    11101 catcatcgcc ctgcgccaga tccactaaaa agcgcggatg aaccgactcc agcacgcgac
    11161 cggggccgtc cagtaacgtc ttggtcaata tcgttctgcc gtgaaaagtg tttgaatatc
    11221 atcgcgtaac agctgagcgt cggtgtaaat ccagctgtga gtcatcacag tctgctgcaa
    11281 ttgctgctgc atcagcctga ccaccgattc attttgttga cgcagagcca gactttcgcg
    11341 taaacgcgtc tgtaattctg tcaaacatga agcgaactca gcgaaaaaag tattcatgcc
    11401 tgccgtaaca gattcatcga cctgctctgc cagaacttta gccatttgtt ggcaatagag
    11461 atcgacttct gcgcttaatg ctcgttgcaa cacactgtaa tcaaccgttt ctgtcgggga
    11521 tttctcattt ccccgtcccc agtcgggctg attcaaccag cgcgaaaaag tctcacgcac
    11581 aacgcctaaa cgcgtgctct gctcgtccgt tgcttcctgg cgcgaaatga cttcactgaa
    11641 cagctggcga gtgttgaagt ggggaactac gccgtgaaaa acaggaaaat gaaacccagg
    11701 acgaaaccct gactcgctca attccatttt gacttgttgc tcaatggggc gaataacatc
    11761 ggttaacacc cggcaaaggg tggattccag ctcggcaaaa cgcagcgtaa agtcgcgact
    11821 gatggtgttc tgcgccgtct gtaacagtgt ctcacagcgg gtacgaatct cgcttaacgg
    11881 ctccgaatca tcctgaaaca aggcggctaa ctgcgcattc accgcatctt gctgttgacg
    11941 cagaaagtgg ttggcggagg tcagggccag ctcgatttca tgtttaatct cgccgctcac
    12001 ctgcgcctga ttgagttgca atagctgcaa actttcttcg acctgatgga tattttgccg
    12061 caattgttca caagcgacgt ttaacccgtg cgcacgaaaa tccaggtatt cccgcgcctg
    12121 ctgcgcgtaa ttcaacagtt tatgcgcagc agatcgcaga gcatacaatg aggcgttagc
    12181 gtaagcggca tgaagcaacg cctgaattgg ctgggcgaac agcgaatctt cccacaactg
    12241 atcggcagca tgacgaatat gttcgaggtc cgccagatcg gcatgacgcc agcgcctgcc
    12301 gagcgcggca tgggcaaaat cttccaccca gcgttgttgc tctggcgctg gtaacttacc
    12361 gttgttggct aactcatagc gcgcccgatt cgccaggtag ccccacatcg acgacaccgg
    12421 gaatatctgc tgtggcgtaa tacagccttt catcagcgtc ccggaaatca gtgcccgcac
    12481 ctggtcggcg tcgtcactgt tacgatcctg ttgatcgaac ttattgacca gcacatacag
    12541 cggcaccgat tgccccaccg ccaaaatcgc ctcacggacc tcttcatcgg agatcgattt
    12601 cagttgcgta taatccagca ccgccagtac cgccgaggcg cgtgccagct gctggttaag
    12661 cattttttgc agatgcggtt gcccggcttc atttggcccg ggggtatcca gtaacgtcaa
    12721 ctgaccggga taactttcca gccccgccag atggacaaac tccacttcaa tcacgggaat
    12781 atgctcaatg gcggcgtaag cagaaaaagg aaaatcgacg tccagcgcct tcgccagtcg
    12841 cactaaatca ttcaaacttt tcagacaatg aaaaataggc tgggcaccca gataatattt
    12901 ttcgaaagcg acgccatttt cgatccgctg cataagcgca cgcatatctt tatctatttc
    12961 cagcacatcg gtcagatgct taatatcgca atcacgcagg cgctgttgta attgttgaat
    13021 taaacaatcg attggcgcga catgtgaaaa atgcagtacc ggttcctttt gcccgggcgt
    13081 atggcgaata agcgtcggca gcgcagtcat tgggcgatta cgattaggca gaacctccgt
    13141 accaacaatg gcattaatgg tggttgattt ccctgctttc atggtaccga caattgccag
    13201 caccatttcc agtcgggaaa ttttacgcaa ctcattattc agcatcgcgt gacgttcggc
    13261 gatattaggc tgactccagg gtaaagccag ttgtggcgcg tcgtctccgg gtacagagag
    13321 aggcattttt tccagtaact gcaactgttg gcgagaaagc tgtaacaggc gttcagcctc
    13381 ctgacttaac tcatacaggg tctgtgtgta catagaaaat tcttccttaa agcaaatttt
    13441 gttactttta tttagccaga ttgtttttga gttctgtttt cggcttttat aattactgca
    13501 agaaataatt ttatatttag tgtgttgttt tttatcagaa taaataacgt cttctgatgc
    13561 gtttaaaacg tcagaaagat aaaaatatca tgtaaataaa aaaaagaaca agtagagcat
    13621 taacattatc ttaaataata aatagaggca aaaagattat tttctttttg cgtttccttt
    13681 caaatgaaaa cgatcgtcgt ctaaaatcag cagtaccccc gacaaactca gggattttgt
    13741 gtataattgc ggcctttttc ggcaatctgc cgttttttgg cgcttttgcc ctgctgactt
    13801 ttgaggaaat ccacatgtca ttaccacact gcccaaaatg caactccgaa tacacttacg
    13861 aagataacgg catgtacatc tgcccggaat gtgcctacga atggaacgac gcagaacctg
    13921 cacaggaaag cgacgagctg atcgttaaag atgctaacgg caatctgctg gctgacggcg
    13981 acagcgttac catcattaaa gatctgaagg tgaaaggtag ctcttcgatg ctgaaaattg
    14041 gcaccaaagt gaaaaacatc cgcctggttg aaggcgacca taacatcgat tgcaaaatcg
    14101 acggttttgg tccgatgaaa ctgaaatctg agtttgtgaa aaagaactga ttgtattgtg
    14161 atcggtaagc cgaataaggc gctcgcgccg catccggcaa cgatgccgac tgcctgatgc
    14221 gacgcttgcg cgtcttatca ggcagcctct catctgtata aatttcgaac tacacttaac
    14281 tggcttctct taactgaggt caccatcatg ccgttaagtc cctacctctc ttttgccggt
    14341 aactgttccg acgcgattgc ctattatcaa cgtacgttgg gcgcggaact gctctataaa
    14401 atcagcttcg gcgaaatgcc gaaatcagcg caggacagcg ccgagaactg cccttccgga
    14461 atgcaatttc ccgataccgc catcgctcat gccaacgtgc gcattgccgg aagcgacatc
    14521 atgatgagcg atgctatacc gtcaggaaaa gccagctact ccggctttac gctggtgctc
    14581 gattcgcaac aggtcgaaga aggaaaacgc tggtttgata accttgccgc taacggaaaa
    14641 atcgaaatgg cctggcagga aactttctgg gcgcatggct ttggcaaagt caccgataaa
    14701 tttggcgtac cgtggatgat taatgtcgtc aaacaacaac caacgcaata acccgccggg
    14761 aggcccgccc tcccgcactg tcatcgaatt cccgttaact cttcatctgt tagtcacttt
    14821 taattaacca aatcgtcaca ataatccgcc acgatggagc cactttttta gggaggctgc
    14881 atcatgcaaa cgattatccg tgtcgagaag ctcgccaaaa ccttcaatca gcatcaagcg
    14941 ctgcatgcgg ttgatctgaa cattcatcac agtgaaatgg tggctctgct tgggccgtcg
    15001 ggttccggca aatccaccct tttacgtcat ttaagcggtt tgattaccgg cgataaatcc
    15061 gccggtagcc atatcgagct actgggccgc actgtccagc gcgaaggccg cctggcccgc
    15121 gatatccgca aaagccgcgc caacaccggc tacatctttc aacaattcaa cctggtgaac
    15181 cgcctgagcg tactggagaa cgtgctgatt ggcgcgctcg gcagcacgcc gttctggcgc
    15241 acctgtttta gctggttcac cggcgagcag aaacagcgcg cgttacaggc gctgacccgc
    15301 gttggcatgg tgcattttgc ccatcagcgc gtttccaccc tctccggcgg ccagcagcaa
    15361 cgtgtggcga ttgcccgtgc gctgatgcag caggcgaaag tgattctggc cgatgaaccc
    15421 atcgcctcgc tggacccgga atcagcgcgc atcgtgatgg acaccctgcg cgacatcaac
    15481 cagaacgacg gcatcaccgt ggtcgtcacg ctgcatcagg tggattacgc cctgcgctac
    15541 tgcgaacgca tcgtcgccct gcgtcagggg cacgtcttct acgacggcag cagccaactg
    15601 tttgataacg aacgttttga ccatctctac cgcagcatta accgcatcga agagaacgcg
    15661 aaagctgcct gatatcccca tcattgagga aaacgaatga acgctaagat aattgcctcg
    15721 ctggccttca ccagcatgtt cagcctcagc accctgttaa ccccggcaca cgccgaagag
    15781 caggaaaagg cgctgaattt cggcattatt tcaacggaat cacagcaaaa cctgaaaccg
    15841 caatggacgc cgtttttaca ggatatggag aagaaactgg gcgtgaaggt caacgccttc
    15901 tttgccccgg actacgcggg cattatccag gggatgcgct tcaataaagt ggatattgcc
    15961 tggtacggca acctgtcggc gatggaagcg gtggatcgcg ccaatggcca ggtcttcgcc
    16021 cagacggtcg cggcggatgg atcgccgggt tactggagcg tgttgatcgt caacaaagac
    16081 agcccgatca acaacctgaa cgatctgctg gcgaagcgga aagatctcac cttcggcaat
    16141 ggcgatccta actccacctc tggcttcctc gtccccggtt actacgtctt cgctaaaaac
    16201 aatatctccg ccagcgactt caagcgcacc gtcaacgccg ggcatgaaac caacgcgctg
    16261 gccgtcgcca acaagcaggt ggatgttgcc accaacaaca ccgaaaacct cgacaagctg
    16321 aaaacctccg cgccggagaa gcttaaagag ctgaaggtga tctggaaatc gccgctgatc
    16381 ctaggcgatc cgatcgtctg gcgcaagaat ctgtccgaaa ctaccaaaga caagatctac
    16441 gacttcttta tgaactacgg aaaaacgccg gaagagaaag cggtgctgga acgcctgggc
    16501 tgggcgccgt tccgcgcttc cagcgacctg caactggtgc cgattcgcca gctcgcactg
    16561 tttaaagaga tacaaagcgt gaaaggcaat aaagggctga atgagcagga caagctggcg
    16621 aaaaccaccg agattcaggc gcagctggat gacctggacc gcctgaacaa cgcgttaagc
    16681 gcgatgagtt cggtgagtaa agcggtgcag taaatcgtag gtcggataag acgccccggc
    16741 gtcgcatccg acaatgtgca ggcgttgatg ccggatgcgg tgcaagcacc ttatccggcc
    16801 tacagaccgg agccaaacat gcaaaccatc accatcgccc cacccaagcg tagctggttc
    16861 tcgcttctga gctgggccgt tgttctcgcc gtgctggtcg tctcgtggca gggcgcggaa
    16921 atggccccgc ttacgctgat taaagacggc ggcaacatgg caaccttcgc tgccgacttc
    16981 ttcccgcccg atttcagcca gtggcaggat tacctcaccg aaatggccgt cacgctgcaa
    17041 atcgccgtct ggggcaccgc gctggcagtg gttctctcca tccccttcgg tctgatgagc
    17101 gccgaaaact tggtgccgtg gtgggtttac cagcccgttc gccgcctgat ggacgcctgc
    17161 cgcgccatta acgaaatggt cttcgccatg ctgttcgtgg tcgccgtcgg cctcggcccg
    17221 ttcgctggcg tgctggcgct gtttatccac accaccggcg tgctctccaa gctgctttcc
    17281 gaagcggagg aagcgatcga acccggcccg gtggaaggca ttcgcgccac cggcgccaac
    17341 aaactcgaag agatccttta cggcgtgctg ccgcaggtga tgccgctgct gatctcctac
    17401 tccctctatc gcttcgaatc caacgtccgc tcggcgaccg tcgtcggcat ggtcggcgcg
    17461 ggcgggatcg gcgtcaccct gtgggaagcg attcgcggtt tccagttcca gcaaacctgc
    17521 gccctgatgg tgcttatcat cgtcacggtc agcctgctgg atttcctctc tcaacggttg
    17581 cgtaagcact ttatctgata agcgaggcat tgatatctat gcacttgtct acacatccga
    17641 ccagctaccc aacacgctat caggagatag ccgcaaaact tgagcaggag cttcgtcaac
    17701 actaccgctg cggcgactat cttcccgccg agcagcaact ggcggcgcgc tttgaggtga
    17761 accgccacac cctgcgccgc gccatcgacc aactggtgga aaaaggctgg gtacagcgcc
    17821 gtcagggcgt cggcgtgctg gtgctgatgc gcccgttcga ttacccgctc aacgcccagg
    17881 cgcgttttag ccagaacctg ctggaccagg gcagccatcc caccagcgaa aaactgctgt
    17941 cggtattgcg ccccgcgtcc ggtcacgttg ctgaagcgct ggggattacc gagggcgaga
    18001 acgttatcca tttgcgcacc ctgcgccggg tcaacggcgt cgcgctctgt ttaatcgacc
    18061 actactttgc ggacctcacc ctttggccga cgctgcaacg cttcgacagc ggttcgctgc
    18121 acgattttct gcgcgagcaa accggaattg cgctgcgccg cagccagacg cggatcagcg
    18181 cccgccgcgc ccaggccaaa gagtgccagc gccttgaaat cccgaatatg tcgccgctgc
    18241 tgtgcgtgcg cacccttaac caccgtgacg gtgaaagcag cccggcggag tactccgtca
    18301 gcctgacgcg cgccgacatg attgaattca ctatggagca ctgaatgcac gcagataccg
    18361 cgacccgcca gcactggatg tccgtactgg cgcacagcca accggctgaa ctggcagccc
    18421 gcctgaacgc gctgaacatc accgccgact atgaagtgat ccgcaccgcc gaaaccggcc
    18481 tggtacagat tcaggcgcga atgggcggca ccggcgaacg cttttttgcc ggcgacgcca
    18541 cgctgacccg cgccgccgta cgcctgactg acggcaccct cggctacagc tgggtgctgg
    18601 ggcgtgataa acagcacacc gaacgctgcg cgctgattga tgcgctgatg cagcaatctc
    18661 gccactttca aaacttatca gaaaccctta ttgccccgct ggacgctgac cgtatggcac
    18721 gcattgccgc acgccaggcc gaagtgaacg ccagccgggt cgacttcttt acgatggttc
    18781 gcggagacaa cgcatgaccc tggaaaccgc ttttatgctt cccgtgcagg atgcccagca
    18841 cagttttcgt cgcctgttaa aggccataag cgagccgggc gtgattgtcg ccctgcatca
    18901 gctcaagcgc ggctggcagc cgctgaatat cgccaccacc agcgtgctgc tgacgctggc
    18961 tgataacgac acgccggtgt ggcttgccgc gccattgagt aacgatatcg tcaatcagag
    19021 cctgcgtttt cataccaacg cgccgctggt cagccagccg gaacaggcga ccttcgcggt
    19081 gacggatgac ggaatttcca gcaaacagct caacgccctt tccaccggca ccgccgttgc
    19141 gccggaagcg ggcgcgacgc tgattttaca ggtcgccagc ctgagcggcg ggcgcatgtt
    19201 gcgtctcacc ggcgcgggta ttgccgaaga acgaatgatc gctccgcagc tgccggagtg
    19261 cattctgcac gaactcaccg agcgcccgca cccgttcccg ctcggcatcg acctgatcct
    19321 gacctgcggc gaacgcctgc tggctattcc gcgaaccact catgtggagg tgtgctgatg
    19381 tacgttgccg tgaaaggggg cgaaaaggcg atcgacgccg cccacgccct gcaagagagc
    19441 cgacgccggg gcgataccga tttgcccgaa ctgagcgtcg cccaaattga acagcagctt
    19501 aatctcgcgg tggatcgcgt gatgaccgaa ggcggcgttg ccgaccgcga actggcggcg
    19561 ctggcgctga aacaggccag cggcgataac gttgaagcaa ttttcctgct gcgcgcctac
    19621 cgcaccacgc tggcgaaact ggcggtaagc gagccgctcg acaccaccga gatgcgtctc
    19681 gaacggcgta tttcggcggt ttataaagac attcccggcg gccagctgct tggcccaacc
    19741 tacgactaca cccatcgcct gctcgatttc accctgctgg caaacggtga agcgccgacg
    19801 ctgaccaccg ccgacagcga acaacagccg tcgccgcacg ttttcagcct gctggcgcgt
    19861 caggggctgg cgaagtttga agaagatagc ggcgcacagc cggatgacat cacccgcacg
    19921 ccgccggttt acccctgctc acgctcctcc cgcctgcaac aattaatgcg cggggacgaa
    19981 ggctatttgc tggcgctggc ctactccacc cagcgtggtt acggacgcaa tcacccgttc
    20041 gcgggcgaga tccgcagtgg ttacatcgac gtgtcgattg tgccggaaga gctgggattt
    20101 gcggtaaacg tcggcgaact gctgatgacc gagtgtgaaa tggtcaacgg ttttatcgac
    20161 ccgccggatg agccgccgca cttcacgcgc ggctacgggc tggtattcgg catgagcgag
    20221 cgcaaagcga tggcaatggc gctggttgac cgtgcgttgc aagccccgga atacggcgag
    20281 cacgcgacag gcccggcgca ggatgaagag ttcgtgctgg tgcatgccga caacgtcgaa
    20341 gccgcaggct ttgtctcgca cctcaaactc ccccactacg tcgatttcca agccgaactg
    20401 gagctactca aacgtctgca acaggagcag aaccatggct aatctgagcg gctacaactt
    20461 tgcctacctc gacgagcaga ccaaacgcat gatccgccgc gccatcttaa aagcggtggc
    20521 tatccccggt tatcaggtgc cgtttggcgg gcgcgagatg ccgatgccct acggctgggg
    20581 aaccggcggc attcagctca ccgccagcgt gattggcgaa agcgacgtgt tgaaggtgat
    20641 tgaccagggc gcagacgaca ccactaacgc cgtgtcgatt cgcaacttct tcaagcgcgt
    20701 gaccggggtg aacaccactg aacgtacgga cgatgcgacg cttatccaga cgcgccatcg
    20761 catccccgaa acgccgctga ccgaagatca gatagttatt ttccaggtgc cgatccccga
    20821 accgctgcgc tttatcgagc cgcgcgaaac ggaaacccgc actatgcacg tgctggagga
    20881 gtacggcgtg atgcaggtga aactgtatga agatatcgcc cgcttcggtc atatcgccac
    20941 cacctatgct tatccggtga aggtgaacgg gcgctacgtg atggacccgt cgccgatccc
    21001 gaaattcgat aacccgaaaa tggacatgat gcccgccctg cagctgttcg gcgcagggcg
    21061 cgagaagcgc atctatgcgg tgccgccgtt tacccgcgtg gaaagtctcg atttcgacga
    21121 tcacccgttc accgttcagc agtgggatga gccatgcgcc atctgcggat cgacccacag
    21181 ctatctcgac gaagtggtgc tggatgacgc cggaaaccgc atgtttgtct gctccgatac
    21241 cgattattgc cgccaacaga gcgaggcaaa aaaccaatga atcaaccgtt actttcggtc
    21301 aataacctga cccaccttta cgcgccgggc aaaggcttta gcgatgtctc ttttgattta
    21361 tggccggggg aagtgctggg catcgtcggg gaatccggct ccgggaagac cacgctgctg
    21421 aaatcaatct ccgcgcgcct gacgccgcag cagggggaga ttcgctacga gaatcgttcg
    21481 ttgtatgcga tgagcgaggc cgaccgccgt cgcctgctgc gcaccgaatg gggcgtggtg
    21541 catcagcatc cactcgacgg cctgcgtcgc caggtctcgg caggcggcaa tatcggcgag
    21601 cggctgatgg cgaccggggc ccgtcattac ggcgacatcc gcgccaccgc gcagaagtgg
    21661 ctggaagagg tggagattcc cgccaaccgg atcgacgacc tgccgaccac cttttccggc
    21721 ggcatgcagc aacgtttgca gattgcccgc aacctggtga cgcatccgaa gctggtgttt
    21781 atggacgagc caaccggtgg gctggatgtg tcggtgcagg cacgcctgct cgatctactg
    21841 cgcggcctgg tggtggagct gaacctggcg gtggtgattg tcacccatga tttaggcgtc
    21901 gcccgcctgc tagcggaccg tttgctggtg atgaagcagg ggcaagtagt ggagagtggg
    21961 ttaaccgacc gcgtgctcga cgacccgcac catccgtata cccagctgct ggtgtcatcg
    22021 gttttgcaga attgagccgg tgccggatgc ggcgtaaacg ccttatctgg cctacaaatg
    22081 cgctccccgt aggtcggata agacgcatca gcgtcgcatc cgacacccga accacgaggc
    22141 gaaaaatgat taacgtacaa aatgtcagta aaaccttcat cctgcaccag caaaacggcg
    22201 tgcgcctgcc cgtcctcaac cgcgcctcgc tcaccgtcaa cgcgggcgaa tgcgtggtgc
    22261 tccacggcca ctccggcagc ggcaaatcaa ccctgctgcg ctcgctgtac gccaactatc
    22321 tgcccgatga agggcaaatc cagatcaaac acggtgacga gtgggtagac ctggtcaccg
    22381 cgccagcgcg caaagtggtg gagatccgca aaaccaccgt cggctgggtg agccagtttc
    22441 tgcgcgtcat cccgcgtatt tcagcactgg aagtggtgat gcagccgctg ctcgataccg
    22501 gcgtaccgcg tgaagcctgc gccgctaaag ccgcgcgtct gctcacccgc ctgaacgtgc
    22561 cggaacgcct gtggcacctg gcgccatcaa ccttctccgg cggcgaacag cagcgcgtca
    22621 acatcgcccg cggctttatc gtcgactacc cgattctgct gctcgacgaa cctaccgcct
    22681 cgctggacgc caaaaacagc gccgcggtgg tggaactgat ccgcgaagcc aaagctcgtg
    22741 gcgcagccat tgtcggcatc ttccatgacg aagccgtacg taatgacgtt gccgaccgcc
    22801 tgcacccaat ggaagcctct tcatgattat caataacgtc aaattggtgc tggaaaacga
    22861 agtggtacac ggttcactgg aggtgcagga cggcgaaatc cgcgcctttg ccgaaagcca
    22921 gagccgcctg ccggaggcga tggacggcga aggcggctgg ctgctgccgg ggctgattga
    22981 gctgcatacc gataatctgg ataaattctt caccccgcgc ccgaaagtcg actggcccgc
    23041 ccattcggcg atgagcagcc acgacgcgct gatggtggca agcggcatca ccaccgtact
    23101 ggacgccgtg gcgattggtg atgtgcgcga cggcggcgat cggttggaga atctggagaa
    23161 gatgatcaac gccatcgaag agacgcagaa acgcggcgtc aaccgcgccg agcaccgtct
    23221 gctcctgcgc tgcgaactgc cgcatcacac cacgctgccg ctgtttgaaa aactggtgca
    23281 gcgcgagccg gtgacgctgg tgtcgctgat ggaccactcg ccgggccagc gccagttcgc
    23341 caaccgcgag aagtatcgcg aatattatca gggcaaatac tcgctcaccg atgcgcagat
    23401 gcagcagtac gaagaagagc aactggcgct tgccgcacgc tggtcgcagc cgaatcgcga
    23461 atccatcgcc gccctgtgcc gcgcgcgaca aattgcgctc gctagccatg atgacgccac
    23521 ccacgcccac gttgccgaat cccacctgct tggcagcgtg atcgccgaat ttcccaccac
    23581 gtttgaagcg gcggaagcct cgcgcaagca tggcatgaac gtactgatgg gcgcgccgaa
    23641 tatcgtgcgc agcggttcgc actccggcaa cgtggcggcc agtgaactgg cgcagcttgg
    23701 cctgctggat atcctctctt ccgactacta ccccgccagc ctgctggatg cggcatttcg
    23761 cgtcgccgat gaccagagca accgctttac gctgccgcag gcggtgaagt tagtgaccca
    23821 aaatccggcg caggcgctta atcttcagga tcgcggggtg attggcgagg gcaaacgcgc
    23881 ggacctggtg ctggcacatc gcaaggacaa tcgtattcat atcgaccacg tctggcgtca
    23941 gggtaaaagg gtgttctgat gatgggaaaa ctgatttggt taatggggcc gtccggctcc
    24001 gggaaagaca gcctgctggc ggaaatccgc ctgcgggaac aaactcagtt actggtggcg
    24061 catcgctaca ttacgcgcga cgccagcgcc ggaagtgaaa accatatcgc cctgagcgag
    24121 caggagtttt ttacccgcgc ggggcaaaat ctgttggcct taagctggca cgctaacagt
    24181 ctgtattatg gcgtcggcgt tgagattgat ctctggctgc acgccggatt cgacgtgctg
    24241 gtcaacggct cacgcgccca tctgccgcag gcgctggcgc gctatcaatc ggcgctgctg
    24301 cccatttgct tacaggtttc gccggagatc ctgcgccagc gcctcgaaaa ccgtggtcgt
    24361 gaaaatgcca gtgaaattaa cgcccgacta gcacgcgctg cccgctatac tcctcaggat
    24421 tgtcttacgc tcaataatga cggcagcctg cgccagtcgg tcgacaagct gctgacgctg
    24481 attcatcaga aggagaaaca ccatgcctgc ttgtgagctt cgcccggcta cgcagtacga
    24541 caccgacgca gtttacgcgc tgatttgtga gctaaaacag gcggagtttg accaccatgc
    24601 gtttcgcgtg ggctttaacg ccaacctgcg cgacccaaac atgcgctacc atctggcgct
    24661 gctggatggc gaagttgtca gcatgatcgg cctgcatttg cagtttcatc tgcatcatgt
    24721 caactggatc ggcgaaattc aggaactggt ggtgatgccg caggcgcgcg gtctgaacgt
    24781 cggcagtaaa ttactggcat gggcagaaga agaagcccgc caggccgggg ccgaaatgac
    24841 cgcgctttcg accaacgtga aacgccacga cgcgcaccgt ttctatctgc gagaaggcta
    24901 cgagcagagt cacttccgct tcaccaaggt gctgtaacat gagcctgacc ctcacgctca
    24961 ccggcactgg cggcgcacag ggcgttccgg catggggctg cgagtgcgcg gcctgcgacc
    25021 gagcgcagcg ctcaccgcag tatcgccgcc agccatgcag cggcgtagtg aagtttaacg
    25081 acgcaatcac cctgatcgac gccgggctgc acgatctcgc cgatcgctgg tcgcccggat
    25141 cgttccagca gtttttgctg acgcattatc atatggatca cgcccagggg ctgtttccgc
    25201 tgcgctgggg cgtgggcgat ccgatcccga tctacggccc gccggatgac cagggttgcg
    25261 acgatctgtt taaacatccg ggcctgcttg atttcagcca cacagtggaa ccgtttgtgg
    25321 tgtttgattt gtaggggttg caggtcacgc cgctgccgct taaccactca aaactgacct
    25381 tcggttatct gctggaaacg gcacatagcc gggtggcgtg gctatctgac accgctggcc
    25441 tgccggaaaa aacgctgaaa tttttacgca ataatcagcc acaggtaatg gtgattgatt
    25501 gcagccaccc gccgcgcgcg gatgcgccgc gtaatcactg tgatttaaat accgtgcttg
    25561 cgctgaatca ggttatccgc tcgccacggg tgattctgac ccatatcagc caccagtttg
    25621 atgcgtggct gatagaaaac gcactgccgt cggggtttga ggcggggttt gatgggatgg
    25681 agattggggt ggcgtgatga gagggaatgt gcgcgctggt cccctcaccc taaccctctc
    25741 cccagagggg cgaggggagc gattgtgctc gatattgaat attgcgctgg ttttctccct
    25801 ctccccattg gggtgagggg cgatgcctgc tccataccca acctcatcgc ccatactcat
    25861 cttccattct ccgctcttca tcttctagct gccgacgctc ctgatcaagc tggcgctggc
    25921 gatcgtccag ctgcctgcgg cgatcttcaa actggcagcg gcggtcgtca tattgtctgc
    25981 gccgatcgtc gctcacttca cgctgccagc cgtcgtcgcg cgaatcttca tagtctcgcc
    26041 cacggtcaga gttataagcg tcattaatcg cctgctgaat attaccaata gtatcgtcga
    26101 taatatcggc ctgggccgga acggtggaca gcgtgagcag ggtgaaaaga aaaagcgtgg
    26161 agtaaggttt cataagccag tctctgcgat ggactggctt aacgttagtt gagtgtgaat
    26221 gaccggagta gcggagtaat ctcaaattcg gtaaggcgca gactcttcca ttagctggtt
    26281 agctcgctgt ttattttccg ccaccaatac aatgccatga catttttgat ccggtgaaat
    26341 actccagata cgctcttccg gaatgcagcc gccatcgtag atcactttcg tgccatcgta
    26401 gggccaaaat gccccttgtt cagtgtctaa tacaaacacg gaaatctgat cggactcgcg
    26461 cgcgatcttc tgccagagca tcttcccgcc atcatattga tcaaaatcgc tgattagcgt
    26521 gataccgcat tgcaggatta gcgcttcata cagccgagtt gccagaccct tatcgcgctc
    26581 acctggagag acacaaatac cttccacctg taccagatcc gagtcttccc accagcgttc
    26641 aacggcaatg atatcgctgc cgaactcttt tttacgctgc agatcgagag cggcaagtac
    26701 ccgatgacgc ggcacgcctg caaaaacttc aatgtccgct tcaatgatgg ccactcttgg
    26761 cgtctggttg tcataaatta cctgtaacag acgtccagaa agctcgatct cttccaccac
    26821 gcgaaaagaa cgagcaaccg tcatagggct ataggtggtt ggcatcacga tcatatgtaa
    26881 atcacttcaa ttcagaacag aattgctcac aatagcagca cgtttgcttc gagcctcttg
    26941 ataaattctg aaaatcatcc ctcaatttgc cccagcacct gcgccagctg cgcgcgggta
    27001 aagggtttgc gcagcagcgc cacgtccggc agcgcggggt tatggctggg gcgtaaatcc
    27061 tgaccgctga tgagtagcag cgtcaggtgc gggtaaagtt tgcgggccgc attgaccact
    27121 tctgcgccgc tcatgccgcc agcaggtcca gcgcctgttc gccgctgctc gcttccagtg
    27181 tcaaatagcc cagcaggtgc aactgctcgc agatggtctg gcgcaccgcg gcttcatctt
    27241 ccagcactaa caccagttta tcgccattaa cagctgtctg ttctaccgcc gctggcgata
    27301 ggtttttcgc cgccgtcagc gcgcggggaa gctgtaatct gaccgttgtc ccctgccccg
    27361 gcgcgctttc aatttccacg cgcccgccgg actggcgcac gaaaccatag accatcgaca
    27421 agcccagccc gctgccgctc cccgtctgtt tggtggtaaa gaacggctcg aacacctggc
    27481 ttttcaccgc ctgcgacata ccgctgccat gatcgatgac ctccagtgcc accatgtcct
    27541 gacgctgccc actgctgcgg gtaacacgct gattccaggt acgaattttg ataattcccg
    27601 tctgcccttc catcgcgtcg cgggcgttca tcaccaggtt gatgatggcg ttttccagtt
    27661 ggctgacgtc aatccacgcg gaccacgccg gagactgcgc ttcaatctca atcgtcagcg
    27721 tcgccggtaa ggagtggcgc attaattcgc ttaactcttc cagcagtgtt tttagttcaa
    27781 cggcttgcgg atgtaaggac tgcttgcggg aaaatgccaa caatcgctgg gtcagtagcg
    27841 cgccacgttc ggcggcttgc tgcgcccgct gtattcgcgg cgcgtcgggt gagttcgtgt
    27901 tcaccagttc caggctacca ataattaccg ccagcagatt attaaaatca tgggccagcc
    27961 cgcccgtcag ttgcctgacc gcttttaact tctggctgtg caatagcgcc tcttccagcc
    28021 cctgccgctc ggtttgggca atctccatct gcgtggtttt ctctttcaac agccgtgacg
    28081 ttcgttcaag tgatgcggta ttgcgggcaa aaacgttaaa ggcgcgcgcc agttcaccca
    28141 actcatcgcg ccgctgtaac gccggaaaag tgacgtccgc ttcgccgtgc gccaggcggc
    28201 tcatcgcgcg ggaaatcgcc gtcaggttag agccgagatt gcgatagata taccaacctg
    28261 caaacgcggt gatcaccacc gctaacaggg cgaaaatcat gatcagcctc tcgccggagc
    28321 gcaaatcctg gtaactttgc tccacccgct gaccagagag cgtcgccacc tgttcggcgt
    28381 aacggttgat gtcgccatta aggatcgcca ccagcgcttt gatgtgaaac atataccagc
    28441 tgattgccag atcgctttgc tccagagccg ccgaaagtgg cgcgagttta cgcagttcac
    28501 gattaaaatc agcgagtagc gtattaacca atggcgtatc gctgtgagtt ggcaaggcag
    28561 acatcactcc gaccagttga tgaataatcg cctgcggcgc gggggtggca atggcggcaa
    28621 caatcagtcg gttcatctcg ttaagcaaaa tattatcttg cgcgtgagtg actttttgca
    28681 gatgccgcag ataacttaag ttttgataca gcgaacttaa cagcgcgtta cgctccagat
    28741 gccgcctttg cccgcgttcc agcattccgc cgacgctggt ttgcagttcg ttgctacgcg
    28801 tgatgatgcg cgtcaccagc ccggcttcct gctgcgctaa aggcgcgttc gccagttgtg
    28861 caagcgagct ttttaatgcc cgctgtgtct cctgcaaccg ctcggcttcg cctttatatt
    28921 ccagtgctcc caccacctgc gacaatcgca ctgccgccgt tgccacgttt gccgtgtcgc
    28981 gcgccagatt caggctgccg gtcatatcat ctacagtttg ctgctgcacc agctcttgca
    29041 tctggctggc atgacgaaag cccattaccg ccacgccgct gaccatcaac gtcaccgcca
    29101 ccaccagcaa attcaaaagc agcaatcgcc cgcgcgcgct ggcaaaaaag tgacgtccgg
    29161 ggtgcgccat atcacgctcc gtgaagaaaa atcgcaatat gacaaatatg aacggcttct
    29221 gacattttgc atttactttc ctgtgatgga gtgctgatat cgacaacctc gcaggagtcc
    29281 accatgaaca gcacgccggt tcttgagatg cgcaatattg ccaaagcctt cggcaaattt
    29341 tatgcgctca aaggggtgga tttgacggtc taccctggcg agatccatgc cctgatgggg
    29401 gaaaacggcg cgggaaaaag cacgctgatg aaggtgctgg cgggggcgta taccgccacc
    29461 agcggcgaga tcctgataga cggcaagccc tttcacattc gcacgccaaa agatgcctta
    29521 agcgccggta ttacgctgat ttatcaggag atgcagctgg cacccaattt gacggtggca
    29581 gaaaatattt tcctcggcag cgagctttcc cacggcgggc tggtgcagcg aaaagagatg
    29641 ctggtgcagg cgcaaagagt gatcgaccgc ctcggcgcgc agtttaacgc cagcgataag
    29701 gtcatgacgc tgaccattgc cgaacaacag caggtcgaaa tcgcccgcgc gctacatcgc
    29761 aacagccgca ttctggtgat ggacgaaccc accgctgccc tctcctcccg tgaaactcac
    29821 cgtctttttg aactgattat gcggctgcgc gatgaaggga tggcgattat ctacatcagc
    29881 caccgtatgg cggaagtgta tgagctttcc gatcgcgtca gcgtgctgcg cgacgggcaa
    29941 tacgttggca gcctgacccg cgataacctc aatgccgggg agctggtgcg gatgatggtc
    30001 ggcaggccac tgagcgatct gttcaataaa gagcgcgata tcccgctcgg taaagcccgc
    30061 ctgaatgttc accacctgac cgacggcggc aaagtccagc cgagctgcct gcaggtgctt
    30121 tccggcgaaa ttgttggcct cgccgggctg gtgggagccg gacgttccga actggcgcag
    30181 ttaattttcg gcgtgcggaa agcgacaggc ggaatgattg aagtcgatgg cgaaccggtg
    30241 gtgatccact ccccgcgcga agccatcgat cttggcattg gttttctcac cgaaaaccgc
    30301 aaagaacaag gcttattcct tgaaatggcg gcagtcgaaa acatcactat ggcaaccctg
    30361 gagcgcgatg ccagctgggg aatgctcaat cgcaaaaaag cgcaaaccat ttccgatgac
    30421 gccattaagt tgctcaacat tcgcgtgcct catgcccagg tacgcgcggg cgggctttcc
    30481 ggcggcaatc agcaaaaact gttgatctcc cgctgggtgg cgattggccc gcgcatttta
    30541 ctgctcgatg aacccacccg cggcgtggac gttggcgcaa aaagtgagat ctaccggatc
    30601 atgaacgaga tggcgcgcaa gggcgtggcg atcctgatga tctccagcga actgccggag
    30661 atagtgggaa tgagcgatcg cgtccatgtg atgcgcgaag gcagcattgc cggtgagcta
    30721 aatggcaaaa acatcaccca ggaaaacatt atgaccttag cgactggcgt caacgacgcc
    30781 cattcccagg cggtgacctc atgacgacct ctgcaaaaac ctctgccaaa aaaatgctga
    30841 tgagcgatct gatgcaaacg gtcggcattt tgcccatttt gatcctgatc gtagcggtat
    30901 ttggctttat cgcgccaaac ttttttaccg aaagcaacct gctaaatatt acccgccagg
    30961 cgtcgatcaa cattgtgctg gcggcgggga tgaccttcat cattttgacc ggtgggatcg
    31021 atctttctgt aggttcaatc ctcggcacca cggcggtggc ggcaatggtt gtctccctta
    31081 gcccggagtt cgccatgttg tctatcccgg cggcgctgat gctcggcctg ctgctggggc
    31141 tgttcaacgg cgcgctggtg gcctttgccg gattgccgcc gtttatcgtc acgcttggca
    31201 cctatacggc gctgcgcggg gcggcgtatc tgctggctga tggcaccacg gttatcaact
    31261 ccaacattaa tttcgagtgg ataggcaata actatctcgg gccggtcccg tggctggtgg
    31321 tgattgcgct ggcggtgatc gtgctgtgct ggtttattct gcgtcgcacc acgcttggcg
    31381 ttcatatcta cgccgtcggt ggcaatatgc aggcggcgcg tcttaccggc attaaagtct
    31441 ggctggtgct gctgtttgtt tatggcatga gcggcctgct ttcagggctt ggcgggatta
    31501 tgagcacctc gcggctgtac agcgccaacg gcaacttagg tatgggttac gaactggatg
    31561 ccattgcggc ggtgatcctc ggcggcacca gttttgtcgg cggcatcggc accatcaccg
    31621 gcacgctggt gggggcgcta attatcgcca cgctcaacaa tggcatgacc ttaatgggcg
    31681 tctcttactt ctggcagctg gtgatcaaag gggcggtgat catcattgcg gtgcttatcg
    31741 acaaataccg tacccgacat catcaaagtg cataacaaca ataccctaca catgaggaat
    31801 acagcatgag attgaagccc ttagtgaccg cgctctgtgc aggcgcgctg ctggccgcag
    31861 caccgttcgc ccaggcgaaa gatctgaaat cgataggcgt aacggtaggt gacctggcga
    31921 acccgttctt cgtacagatc accaaaggcg cggagctgga agcacgcaaa ctggcgggcg
    31981 ataacgtcaa agtgacgctg gtttccagcg gctacgatct gggtcagcag gtctcgcaga
    32041 tagacaactt tatcgccgct aacgtcgata tgatcatcct taacgctgcc gattcaaaag
    32101 ggatcggccc ggcggtgaaa cgggcaaaag acgccgggat cgtggtggtg gcggttgacg
    32161 tggcggcaga aggtgccgat gccaccatca cttccgataa cacccaagca ggcgaaatgg
    32221 cctgtaaata catcaccgat cgcctgaaag gcaaaggcaa cgtggtgatc atcaacggac
    32281 cgccggtgtc cgccgtgcaa aaccgcgtcg aaggctgcca gaccgaattt aaacgccatc
    32341 ctgacatcaa ggtgctctct gacaacaaga acgccaaagg cagtcgtgag ggcgggctgg
    32401 aggtgatgac cgggctgctg gcggcaaatc cgaagatcga cggcgtattt gcgattaacg
    32461 atccgacagc gatcggtgcc gatctggcag caaaacaggc gcaacgtaac gagttcttta
    32521 ttgtcggcgt ggatggaagc ccggacgccg aagaggcgct caagcgtgag aactcgttgt
    32581 ttgtcgctac tccggcgcaa gatccacagg tgatggcagc gaaagcggtt gagatcagtt
    32641 atgacatttt gcagggcaaa cctgcgccga aagagccggt gctgatcccg gtgacgatga
    32701 tcgataaaaa caacgtcggc aattacaaag gctggacggt gaaataagtc cacacgccgg
    32761 gcggcgatgc tgcccggcca ccgctaagga gaacgctttc gatgaaacgc tccgctataa
    32821 acaacattct cggccataca cggcaattct tttcgcaaca tgacgtacat ctgccgccct
    32881 ttgccagttt ctctcctgct caatggcagc agctcgacac cgccgcgtgg gaggtggttt
    32941 ttgatctcaa actcggctgg gatgttaccg cttttggtgg taacaacttt gcagcacagg
    33001 ggctgatgct gtttaccctg cgtaacggct cagcaaaagg aatgccgtat gtgaaatgtt
    33061 acgccgagaa gatcatgcat gtgcgcgatg cgcaggtcac gccaatgcat tttcactggc
    33121 gtaagcgtga ggacatcatc aatcgcggcg gcggcaattt gattgttgaa ctgtggaatg
    33181 ccgatagcaa cgagcaaaca gttgataccg acgttacggt ggtgatcgac ggctgccgcc
    33241 agactcatgt cgcaggcagt cagttacgcc tgtcccccgg cgaaagtatc tgcctgccgc
    33301 ccggcctgta tcacagcttt tgggcggaaa ccggttttgg cgatgttctg gttggcgaag
    33361 tctcttctgt caatgacgac gaccacgata accacttttt acaaccgctg gaccgctaca
    33421 acctgattga cgaagacgaa ccggcccagc tggtgttgtg taacgaatac cgccagttcc
    33481 tctgaataag gataacgtta tgccgttgat ttctctcgct gaagggctgg cacatgcccg
    33541 cgaacatcac tatgcgttag gtgcgtttaa tgtgctggac tcacactttt tacgcgcctt
    33601 gtttgctgct gccagacaag aacgttcgcc ctttattatc aacattgccg aagtacattt
    33661 taaatatatc tcgctggatt ccctggtcga gacagtgaaa tttgaagcgg cgcgtcaagc
    33721 gattccggtg gtattaaatc tcgatcacgg gctgcatttt gaatcggtgg cgcaagcgtt
    33781 gcggctggga ttcagctcgg taatgtttga tggctccacg ctggggtatg aagagaatat
    33841 ccgccagacg cgggaagtgg tgaaaatgtg ccatgccgtt ggcgtatcgg tagaagctga
    33901 actgggcgcg gtgggtggtg acgaaggcgg tgcgctttac ggtcatgccg atgaatctct
    33961 gtttaccgat ccgcagctgg cgcgtgattt tgtcgaccgt accggcattg acgcgctggc
    34021 cgtggcgatt ggcaatgcgc acggcaaata tcgcggcgaa ccgaaactcg atttcccgcg
    34081 cctcgacgct atccgccagc aggcggctat cccgctggta ttacacggcg gttcagggat
    34141 tagagatgcc gatttccgcc gcgccatcga actgggtatc cacaaaatca atttctacac
    34201 cggcatgtca caagcggcgc tggctgccgt tgaggcccga atggcgcacc gtcagccgat
    34261 gtatgacgag tttgccgagt tactactagc agtggaagcc gccatcagcg atacggttgc
    34321 acaacagatg cgtattttcg gcagtgcggg gcaggcatga tggaacgtaa agggattatc
    34381 gccgcaggca atatgctggt tgaccacgtc caccagatta tccagtggcc agagcgcggc
    34441 tggctggcgg agattaccca cagcgaacgc tccaccggcg gcgcgccgct taacgtcctg
    34501 ctgacactgg cgaaaatgcg caccgggttg ctgttacagg cggtcgggct aatcggtcag
    34561 gacagcgacg gcgattacat tatggcgatg cttgagcaat atcacgtcaa tcgtcagcat
    34621 gtgcagcgca ctacctttgc cccgacgtca atgtcgcagg taatgactga tccaagcggg
    34681 cagcggacgt ttttccattc gccaggtgca aacagacttt tggatcttcc ggcgttcgat
    34741 cagctcgata acaccatgaa gattttccat ctcggctatt tactgttgct cgacagcctg
    34801 gatatgccgg acgatgttta cggcacccgt agcgcacgcc tgctggcgca aatgcgtgat
    34861 actggctttg aaacctcact cgacctggtc tcccgcaagg gcgatccgcg ttatcagccg
    34921 ctggtgttgc ccgcgttgcg ccatctggat tacctggtaa ttaacgaact ggaggccggg
    34981 gagtttagcg ggctggaaat tcggttaccc aacggcgaac cgcatatcgc gcatattgct
    35041 gccgccgcgc gtgaactgtt agatgcgggc gtgcgccagc gtgtggtgat ccactgcccg
    35101 gaaggggcgt ggggagagac cccggaacag tgcggcgtgt ggatcccttc gcaaaagtta
    35161 gaacaacggg aaattatcgg cagcgtcggt gcgggcgatg tattttgtgc cggattttta
    35221 tatggctgcc atgaacgctg gacgcttacc gagagcatca aactggcaca cgcctgcgcg
    35281 cgagcaagcc tgttatgcgc caatgccatc gacggggcga aaacgctgga agagctgcaa
    35341 acagaaatgt cggattaatg cacgtcggca gcgaagacat accccagacc gcgcagagtt
    35401 ttaatcaaca tcggctgatg cgggttgagt tcgattttgc gtcgtaagcg catgattaat
    35461 acgtcgatgg tgcgatcaaa cacctccacg ctgtcgctat gagtgaacgc cagcagctgt
    35521 tcgcggctta atacccgacg ggcattttga gccagggcca gtaacaagcc atattcgccc
    35581 tgagtcagct cgacatgttg ttgtagcgga ctatgcagtt ggcagcgaat ggtatcgaga
    35641 taccagccat tgaatgacag cccggcgttt ttctcctcat taaccgccag cgcgccaacc
    35701 cgtcgcaaca cgctttcagt cgtgcgacca ccacccgtgg ggtgaacggc ttgcagatgt
    35761 aatcatccgc gcccatttcc agcccgacca ccacatcaga ctcactcccc attccggtca
    35821 acatcaccac aggcaatgcc ggacgcagtt tctggatttg ctgcaatacc ctcaggccgt
    35881 tggtatcggg cagcatcata tccagcacca ccagcgcaat gtcaggctcg ccctcaatac
    35941 gcgatatggc ctcctgtccg gtatggctga ctgaaaccgt aaagacatgt tcacttaaca
    36001 cgtcctgcaa cagtgcgcag atagccgtgt catcatccac caccagcacg acgggtttca
    36061 tggttacact ccgcatcatt catctaatta atcattgtgc gtacttcttg tcgaagtgaa
    36121 agcggaaagt aatataaata ataaaaatat aagtcaggtc acattattga tgatagtaat
    36181 aaatatgccg tgcttttccc tcttcagcac aaatgcctaa taaaatggcg cgttccgctc
    36241 acacttccac ttattaaagt cgcaaattcc caattatgat aaccccagct ttaaatgacg
    36301 tattttgcat ggattacata tttacgacac aaatgataaa tggagattta tatgaataac
    36361 tctcggttat tccgtttgag cagaattgtt attgcgttaa ctgccgccag cggcatgatg
    36421 gtaaataccg ctaacgcgaa agaggaagcg aaagccgcca ctcaatatac ccaacaggtt
    36481 aatcagaatt acgccaaatc attaccgttt agcgatcgtc aggattttga cgacgctcag
    36541 cgtggattta tcgctccgct gctggatgaa ggtattctgc gcgatgctaa tggcaaacca
    36601 tattatc
//
LOCUS       DAETPZ010000033        35744 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.033, whole genome shotgun sequence.
ACCESSION   DAETPZ010000033 DAETPZ010000000
VERSION     DAETPZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.033"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..218)
                     /locus_tag="K0W59_001875"
     CDS             complement(<1..218)
                     /locus_tag="K0W59_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBI3022931.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRTLAEVY"
     gene            complement(399..1874)
                     /locus_tag="K0W59_001876"
     CDS             complement(399..1874)
                     /locus_tag="K0W59_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBI3022932.1"
                     /translation="MIQSTRISLGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNEKTNVKLIPQMNYLMVVVALFFLNA
                     IIFLFMLMKCFTNKQILPTLILSLALLSGLIYLVETIVVIHKPISDSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTFFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHNI"
     gene            complement(2021..3300)
                     /locus_tag="K0W59_001877"
                     /pseudo
     CDS             complement(2021..3300)
                     /locus_tag="K0W59_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
     gene            complement(3413..5347)
                     /gene="yeaG"
                     /locus_tag="K0W59_001878"
     CDS             complement(3413..5347)
                     /gene="yeaG"
                     /locus_tag="K0W59_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HBI3022933.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEDNYNGTEGISALPFNGITLAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5783..6529
                     /gene="mipA"
                     /locus_tag="K0W59_001879"
     CDS             5783..6529
                     /gene="mipA"
                     /locus_tag="K0W59_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HBI3022934.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            6619..7380
                     /locus_tag="K0W59_001880"
     CDS             6619..7380
                     /locus_tag="K0W59_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBI3022935.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLVQAGRLRNGLLKNAVVNEIAHV
                     QQNAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(7431..8315)
                     /locus_tag="K0W59_001881"
     CDS             complement(7431..8315)
                     /locus_tag="K0W59_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HBI3022936.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGSVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(8399..9394)
                     /gene="gapA"
                     /locus_tag="K0W59_001882"
     CDS             complement(8399..9394)
                     /gene="gapA"
                     /locus_tag="K0W59_001882"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBI3022937.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            9736..10149
                     /gene="msrB"
                     /locus_tag="K0W59_001883"
     CDS             9736..10149
                     /gene="msrB"
                     /locus_tag="K0W59_001883"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBI3022938.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            10191..10463
                     /locus_tag="K0W59_001884"
     CDS             10191..10463
                     /locus_tag="K0W59_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HBI3022939.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            10833..11909
                     /locus_tag="K0W59_001885"
     CDS             10833..11909
                     /locus_tag="K0W59_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBI3022940.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            11936..13304
                     /locus_tag="K0W59_001886"
                     /pseudo
     CDS             11936..13304
                     /locus_tag="K0W59_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13321..14366
                     /locus_tag="K0W59_001887"
                     /pseudo
     CDS             13321..14366
                     /locus_tag="K0W59_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            14387..15223
                     /locus_tag="K0W59_001888"
     CDS             14387..15223
                     /locus_tag="K0W59_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBI3022941.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            15228..16176
                     /locus_tag="K0W59_001889"
                     /pseudo
     CDS             15228..16176
                     /locus_tag="K0W59_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
     gene            16186..17166
                     /gene="ydjG"
                     /locus_tag="K0W59_001890"
     CDS             16186..17166
                     /gene="ydjG"
                     /locus_tag="K0W59_001890"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HBI3022942.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDPALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            17303..18061
                     /locus_tag="K0W59_001891"
     CDS             17303..18061
                     /locus_tag="K0W59_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBI3022943.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            18179..19537
                     /locus_tag="K0W59_001892"
     CDS             18179..19537
                     /locus_tag="K0W59_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3022944.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFLRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(19630..20271)
                     /gene="pncA"
                     /locus_tag="K0W59_001893"
     CDS             complement(19630..20271)
                     /gene="pncA"
                     /locus_tag="K0W59_001893"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HBI3022945.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(20282..21298)
                     /gene="ansA"
                     /locus_tag="K0W59_001894"
     CDS             complement(20282..21298)
                     /gene="ansA"
                     /locus_tag="K0W59_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBI3022946.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRL
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNVLAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(21465..23321)
                     /gene="sppA"
                     /locus_tag="K0W59_001895"
     CDS             complement(21465..23321)
                     /gene="sppA"
                     /locus_tag="K0W59_001895"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBI3022947.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            23482..24033
                     /locus_tag="K0W59_001896"
     CDS             23482..24033
                     /locus_tag="K0W59_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HBI3022948.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            24150..25193
                     /gene="selD"
                     /locus_tag="K0W59_001897"
     CDS             24150..25193
                     /gene="selD"
                     /locus_tag="K0W59_001897"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBI3022949.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSKMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            25198..27159
                     /gene="topB"
                     /locus_tag="K0W59_001898"
     CDS             25198..27159
                     /gene="topB"
                     /locus_tag="K0W59_001898"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBI3022950.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIEQDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            27286..28326
                     /locus_tag="K0W59_001899"
     CDS             27286..28326
                     /locus_tag="K0W59_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBI3022951.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMRLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIAVLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(28443..29786)
                     /gene="gdhA"
                     /locus_tag="K0W59_001900"
     CDS             complement(28443..29786)
                     /gene="gdhA"
                     /locus_tag="K0W59_001900"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBI3022952.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARAITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDTAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            30022..30294
                     /locus_tag="K0W59_001901"
     CDS             30022..30294
                     /locus_tag="K0W59_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HBI3022953.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(30260..30667)
                     /gene="nudG"
                     /locus_tag="K0W59_001902"
     CDS             complement(30260..30667)
                     /gene="nudG"
                     /locus_tag="K0W59_001902"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HBI3022954.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            30754..31374
                     /locus_tag="K0W59_001903"
     CDS             30754..31374
                     /locus_tag="K0W59_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBI3022955.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKSGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(31383..32690)
                     /gene="ynjE"
                     /locus_tag="K0W59_001904"
                     /pseudo
     CDS             complement(31383..32690)
                     /gene="ynjE"
                     /locus_tag="K0W59_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(32757..33410)
                     /locus_tag="K0W59_001905"
     CDS             complement(32757..33410)
                     /locus_tag="K0W59_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBI3022956.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(33410..34944)
                     /locus_tag="K0W59_001906"
                     /pseudo
     CDS             complement(33410..34944)
                     /locus_tag="K0W59_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(34917..35744)
                     /locus_tag="K0W59_001907"
                     /pseudo
     CDS             complement(34917..35744)
                     /locus_tag="K0W59_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         9073 a         8462 c         9140 g         9069 t
ORIGIN      
        1 cagtacacct cagcgagagt gcgcatcaca tgcgcggcaa tactctgatt ctgatactta
       61 tcataaagaa aggaggagtc tgctttctgc acgatatagc gcagataggc actcattgct
      121 ctgtcgctgg cgacaagttc aaaaataagg aaggaagatg tcagcacaat gacacttgcg
      181 gcgataaaat gccggagcat tctatggtgc aatttcatga taaatggcac tccttcggtt
      241 agtgaaacga gaaaaccaga taaagttcga ttgcggcctg gcacaggggc gctaattata
      301 cttatctctg cgccgctggg gaggggaaat ttaggggtaa tagtaataaa ttgtatgcaa
      361 aagccacgca aaaagcatgg ctctgaatat ttttgcactt aaatattatg aataatcact
      421 ttgttgcccc cctcgctttt cgcttcgcgt aaggctttat ctgcttccgt catgacaagc
      481 gagatattac gagtgtcacc cgttgaaaaa aacgtgccaa tactgatggt catttgttca
      541 ggaacatcat atctgttact gaagccggtc aggagttcga catttttacg catacgttcc
      601 gcaatgattt ttgcctgggc actattgagt tcggtaaata gcaagccaaa cacctcaccc
      661 tccagtcgcg ctaaaatatc atcggggcgg atgctttgct ggataatatc gacaattgaa
      721 accagcactt tatcacccac acgatgtccc cattgggcat tgatgcgttt gaactgatta
      781 atatcaacca gcatgacaca gaagtcctta acaacaggtc gcgacaataa tgactctacg
      841 ctgttgaaaa agtagcgccg attataaata ttggtcagca catcatgaac tgccagtttg
      901 ctggagagtt gcagttcctg aaaaatgtta taaatcagaa aagacacaac aaacagttta
      961 ctggagactt caatcccgcg actgatgtac cagatggtgt aattatactc atccagagtc
     1021 agtagaagta agttgcagca tagcgatgcc agacataata atgctattaa tggccatagt
     1081 tcgctggcta atcgtgtacg catgataata aagaacagta aaaatgccca cagacaaacc
     1141 aatatttttg tatagttgat tccccaggtc gcagtatggt tgtcaggaca gtaatcgacg
     1201 acatacaaag aatagtcagc gttataactg ctcagattgt gtgccagaag gggaaaaact
     1261 aaaaaaggaa tcagcgccag caacagaatg ccagtttttt tcttattgtt gtcaaggatg
     1321 ttgtctttac cataacaaaa gagcgccaac gaggttaaac aaataaaact gagttggcgg
     1381 aaaatataga aaatagaaac atcattcgac tttgtctgga tcagtgtact gtcgctgata
     1441 ggtttatgga taacaacaat agtttcaact aaatagataa ggccacttaa aagtgcaaga
     1501 cttaaaatga gtgttggtag aatttgtttg ttagtgaaac atttcattaa catgaaaaga
     1561 aaaatgatgg cgttaaggaa aaacaaagcc acaacgacca tcagataatt catttgtggg
     1621 atgagtttta cattcgtctt ttcgttatat aatgtggtga tgcaaaataa aattaacgtc
     1681 agcaataaac tgaaaataaa aaagctttga gcttttaatt tttgtgtgcc catagaaacc
     1741 ataaaaagga gagcaatgtg agtgctggat tttattgatg gcagagatat atagttttga
     1801 ccaagatcaa ttttcgttaa cgataaaaaa tatttaaaga ataaccccag ggaaatacgt
     1861 gttgactgaa tcatttttcc gaatgatttt tttctggaac taaattgtgt aaaggttatg
     1921 ctgtcatatt taaagcgatt gtaagctaat gtatgtaata aatgagataa tttacagtaa
     1981 gctaaaaatc agccaggcga taatgacccg gctgatagtt ttagtcttta gctgttgcat
     2041 tttgtttatg aaacagttca cggaacaccg gataaatatc atcctggtcg cggatgtgct
     2101 gcatcgcaaa gttgtcgaaa gtagattgca gatgctcata ttctcgccac aatgtctgat
     2161 gtgcacgacg ggtaatttcg atatagctgt aataacgaac aacaggtaat aattttttcg
     2221 ccaggatttc atggcaaagc ggagagtcat cggcccagtt atcgccgtcc gatgcttgtg
     2281 cagcgtaaat attccactgt gccgggttat aacgctcttt cactacctca tccatcagtt
     2341 tcagggcgct ggaaacaatg gtgccgcctg tttcctgcga gtagaaaaac tcatgttcat
     2401 cgacttcttt cgcctgggta tgatggcgga tgtatacgac ttccacgttc ttatacgttc
     2461 tgctgaggaa cagatacagc agaatataaa aacgcttagc catatcttta gtggattgat
     2521 ccattgaacc ggaaacgtcc atcaggcaaa acatcactgc ctggctggag ggatcgggcc
     2581 gcttctcgta gttcttgtaa cgtaaatcga aggtgtcaat aaaagggacg tgttcaattt
     2641 tggcacgtaa ttctgcaatt tctttgcgca gacgttcctc ttccagcagc tgcgcaggtt
     2701 cactgttgct gatgatggcc aaattctctt ccagtgcatg aagttcccgc cgcttgcctg
     2761 ccgtcatggc tgtgcgtcgc gccagtgagt tctgcaagga acgcacaacg ctgatattgg
     2821 ctggaacgcc gttagcggta taacccgccc gatgcgtttt atattcggtc agctggcgtt
     2881 gttggttttg tttcagattc ggtaaggcca aatcttcaaa gagcagatca agatactcat
     2941 ctttcgaaat ctgaaagaca aattcatcct gaccttcacc atcctggcct ggccctgacc
     3001 actgccggaa cctccgccgc caccctgggg acgttcaatt cggtcgttct gaacgaaatg
     3061 gtcattgccc ggatgcacgc ggtggcgcag accgccacgc ccctgatgaa acattggttc
     3121 gctaatatct tccgtgggaa tggatacgga ttcgctgctg tcgacgtcag tcaccgaacg
     3181 cttattaatg gcctcggaga tcgactgttt aatttgcgct ttataacggc gtaaaaaacg
     3241 ctggcgattc accatgcttt tgtttttgcc gttcagacgc cggtcaataa accaggtcat
     3301 atgccccccg tactacattt gccaactgta tgctacgcct gtgttgccgc ttctcaggcc
     3361 cacggttcgc tatcgttgta ggcccggtag catgtgtacc gggccagggt tgttaagacg
     3421 atttacgtac gcgcaaatac cattcgcaca gtaaacgcac ctgtttacgg gtgtagcctt
     3481 tttccatcat acggtcgaca aagtcgtcgt gtttcttctg ctcgtcggtt gacgttttgg
     3541 cgttaaacga gataaccggc aacagctcct cggtattgga gaacattttc ttctcgataa
     3601 ccgtgcgcag tttttcatag ctggtccagt tcggattgcg tccgctgtta ttcgctctgg
     3661 cgcgcagtac gaagttaaca atctcgttgc ggaaatcttt tggattactg atccccgccg
     3721 gtttctcgat tttctccagc tcggcgttaa gagactcgcg gtcaaacagc tgcccggtat
     3781 ccggatcgcg atactcctga tcctgaatcc agaaatccgc gtaggtaaca taacggtcga
     3841 aaatgttttg cccatattcg gaataggatt caaggtaggc cgtctggatc tctttgccga
     3901 taaactcggc atatttcggg atcagataac ctttcaggaa ctccagatag cgttctgcct
     3961 gctcttgcgg gaactgctcg cgctcgatct gctgttccag gacgtagaac agatggaccg
     4021 ggtttgccgc cacttctaca tgatcgaagt tgaacacgcg ggagaggatc ttaaacgcaa
     4081 aacgcgtcga cagaccgttc atcccttcat cgacaccggc gtagtcacga tattcctgat
     4141 acgacttggc tttcggatcg gtgtctttca gactttcgcc atcataaacc cgcatctttg
     4201 aataaatgct ggagttttct ggctctttca ggcgcgaaag aatggaaaaa cgtgacagtg
     4261 tttcgagcgt gccaggggcg catggggcgt gagtcaattc actgtgatta agcaatttct
     4321 cgtagatttt gatctcttcg gaaatgcgca agcaatacgg caccttcacg atgtaaacac
     4381 ggtcgaggaa ggcttcgttg tttttgttat tacggaaagt gacccattcg gactcgttcg
     4441 agtgggcgag agtaatcccg ttgaacggca gggcggagat accttccgtc ccgttgtagt
     4501 tatcttcctg agtggcggtt aacaagggat gcagcacttt tatcggtgct ttaaacatct
     4561 caacgaattc catgatcccc tgattggcgc ggcacagcgc accggaatag ccgtaggcgt
     4621 ccgggtcatt ctgcgcgtag tgttcgagtt tacgaatatc gactttccca accagcgcgg
     4681 agatgtcctg gttgttctca tcaccgggtt ccgttttggc gatagcaatt tgttgcagaa
     4741 ttgacggcca gaccttcact acccggaact tagtgatatc gccaccaaat tcatgcaggc
     4801 gttttgccgc ccatggcgac atgatggtgc cgagataacg gcgaggaatg ccatactctt
     4861 tttccagaat ctgagcatct tcctgcggat tgaaaagaca gaacggatga tcgttgaccg
     4921 ggctacgctc accgttggcg ctcaatacat aaatcggtac gagctgcatt aatgatttca
     4981 gtcgctcagc aagcgatgat ttacccccac ccacaggccc cagcagatac aggatttgtt
     5041 tcttctcttc cagcccctga gccgcgtgtt tcagataaga gacaatctgt tcaatcgcgt
     5101 cttccatgcc gtaaaactct tcaaatgccg gataacgtgc aatgacccgg ttagaaaaga
     5161 gtcgagaaag tctgggttcc tgggctgtat cgaccatgac aggctcaccg atagccatca
     5221 atagccgctc agccgcgttg gcataagcac tgcgatcttg ccgacaagtg gtaagaaact
     5281 cctgcagtgt gaactcttcg tccttggcag cttcatatcg ctggcgatag tgatcgaata
     5341 tattcatggt atgccgtcct ttcgtttttt agcacaggtt aagagccgtt catatgaata
     5401 gtagaggctc ccggaagagg taaactgaac gacgtctctt gctaaaacac agcaactctc
     5461 atgccatttt atcgacgatg agaacaacgt gcccggtgat acaccttcaa aattaagcgt
     5521 agatggcaaa tggaaaactt gcctgcacgg ctttcttaat ttcaatggca tatcaacaac
     5581 tcatccataa attttgcata attaatgtaa agaccaggct cgccagtaac gctaaatcca
     5641 tttggctgta agcgcggtgt catccgcggc aggaaaatta aacagttact ttaaaaaatg
     5701 aaaacgtaaa aaggttgggt ttcgatgtat tgacgggtaa actttgtcgc ccgctaaaca
     5761 tttgtttaag gaatgattaa ttgtgaccaa actcaaactt ctggcacttg gagtgcttat
     5821 cgcaacgtct gcaggcgttg cgcacgctga aggtaaattt tccctgggcg caggcgtagg
     5881 tgtcgttgag cacccatata aagattacga taccgatgtt tacccagtac cggtaatcaa
     5941 ctatgaaggc gataacttct ggttccgtgg cttaggtggt ggttactacc tgtggaatga
     6001 cgcaacggat aaactttcaa ttaccgctta ctggtcgccg ctttacttca aagcgaaaga
     6061 cagtggcgat caccaaatgc gtcacctgga tgaccgtaag agcaccatga tggctggtct
     6121 gtcttatgct cactttaccc agtacggtta cctgcgtacc accctggctg gcgataccct
     6181 ggataacagc aacggcattg tctgggatat ggcttggttg tatcgttaca ccaacggtgg
     6241 cctgaccgtg actccgggta ttggtgtgca gtggaacagc gaaaaccaga acgaatacta
     6301 ttatggcgta tcgcgcaaag agtccgctcg cagcggtctg cgtgggtata acccgaatga
     6361 cagctggagc ccttaccttg agctgagcgc cagctacaac ttcctcggcg actggagtgt
     6421 ttacggtacc gcgcgctaca cccgtctgtc tgatgaagtt actgacagcc cgatggtgga
     6481 taaatcctgg actggcctga tttctaccgg gatcacctac aaattctgat aatgcatctt
     6541 tccagggcat tttgtgcata aaaatagggc gtcacgccct gttttgcatt acggtggtgc
     6601 aatacctggg aggaaacaat gcaacaaaaa atgattcaat ttagtggcga tgtctcactg
     6661 ccagccatag ggcagggaac atggtatatg ggcgaagatg ccagtcagcg caaaacagaa
     6721 gttgctgcac tacgcgcagg cattgaactc ggtttaaccc tcattgatac cgccgaaatg
     6781 tatgccgatg gcggtgccga aaaggtggtt ggggaagcat taaccggtct gcgagagaag
     6841 gtctttctcg tctctaaagt ctatccgtgg aatgctggcg ggcaaaaagc gataaatgca
     6901 tgcgaagcca gtttacgccg tctcaatact gattatctcg atctctactt attacactgg
     6961 tctggcagtt tcgcttttga agagactgtt gctgcgatgg aaaaattgat cgcccaggga
     7021 aaaatccgtc gctggggcgt ttctaacctt ggttatgccg atatgcagga actctggcag
     7081 ctgccggggg gaaatcagtg tgccactaat caggtgcttt accatctcgg ttcacgaggg
     7141 attgagtacg atctactccc ctggtgccag caacagcaga tgccggtgat ggcttacagt
     7201 ccattagtcc aggccgggcg gttgcgcaat ggactgttaa aaaacgcggt agtcaacgaa
     7261 attgcacatg tccaacaaaa tgcagctgcg cttgaggtcg aactttcgtc agcggaatta
     7321 gctatgctgg ataaggcata tccggcacca aaaggaaaaa ctgcgctgga tatggtgtga
     7381 tgttgatacg tgccggatgc atcgcacccg gcacacaaca ttaacgcaaa ttaacgtttg
     7441 gcaacgcgaa tggattgcgc cagatgtgca ggtttctctt tggtgacttt ttgcgtttct
     7501 gaagcgtaag ccgtttctac acaaacaaag gtcttgtagc catcgtccgg catgtcgccc
     7561 atgctaactg aaagcgccgg tcccgggttc cagccgacaa cgttcagatg atgctggtgg
     7621 cctacggcga taatacgatt cagcgcttca tcattaatca cgctgcaatc ttgcggattc
     7681 agatacacgc gatcggtacg gtcagggaag gtctgaatac cgtcggtcag tacatcttct
     7741 ttcgcgtcat tcactttatc aatgaagcga tcgcccagcc cactgacgct taccttagcg
     7801 atatcaccca cgttaaagta ggtatgcagg gcagaggtgg tttcaaattc gccatgtgat
     7861 tcaagatcga tttcacaggt tttacccacg cggaaatgcg ctaacagcgt aaagtcgtgc
     7921 ggccagaatt ttttcgtctc ttcgctttgt gtcaattcaa aagtcagcgc tacgccatca
     7981 gcatcttcat gatgtgattt cagcgtccac ggcaggttgc gggcaaaacc gtgcgcaggc
     8041 agaccttgtt gtgccgccgg accaaaccac ggccagcaaa ccggtacgct accgcgaata
     8101 gcgacgccat ttttgaacgg tgtgttgttg ctcaaccaca gaacttcttc ttcacccgca
     8161 ggtttccacg agagaaggtg tgcgccctgt aatgcaaaag aggcttttac ctggggatga
     8221 tcgaccacaa tgaggtccag ttcatccagt ttacgacggg agaggacagg ggagatttgt
     8281 tcgatgaccg gaagggcaaa aattttctta atcatgacgc agtcctttaa cttcatttta
     8341 tcaggtaaaa aaaagagcga ccgaagtcgc tctttttaga tcacagtgtc atctcaactt
     8401 atttggagat gtgagcgatc aggtccagaa ctttgttgga gtaaccggtt tcgttgtcgt
     8461 accaagatac cagtttcacg aagttgtcgt tcagagcgat accagcttta gcatcgaaca
     8521 cggaagtgca aacttcgccg ttgaaatcgg tagatactac gtcatcttcg gtgtagccca
     8581 gaacgccttt catttcgcct tcagcagcag ctttaacggc agctttgatc tgctcgtaag
     8641 ttgcagcttt ttccagacga acggtcaggt caactacgga tacgttcggg gtcggaacgc
     8701 ggaacgccat accagtcagt ttgccattca gttctggcag tactttacct acagctttag
     8761 cagcaccggt agaggacggg atgatgttct gggaagcgcc gcggccgccg cgccagtctt
     8821 tgtgagacgg gccatcaacg gttttctgag tagcggtagt agcgtgaacg gtggtcatca
     8881 gaccttcgat gatgccgaag ttatcgttga taactttagc cagcggagcc aggcagttgg
     8941 tggtgcagga agcgttggaa acgatgtcct ggccagcata tttgtcgaag ttagcgcctt
     9001 taacgaacat cggagtgttg tctttagacg gaccagtcat aaccactttc ttcgcaccag
     9061 cggtgatgtg tttacgagca gtttcgtcag tcaggaacag accagttgct tcagcgacaa
     9121 cgtcaacacc cacttcgtcc catttcaggt tagccggatc acgttcagcg gtaacacgga
     9181 tttttttacc gttaacgatc agatgaccgt ctttcacttc aacggtaccg tcgaaacggc
     9241 cgtgagtgga gtcatatttc agcatgtatg ccatgtaatc agcgtctaac aggtcgttga
     9301 ttgcaacgat ctcgatgtca gaacgtttct gagcagcacg gaaaacaatg cgaccgatac
     9361 ggccaaaacc gttgatacct actttgatag tcatatattc caccagctat ttgttagtga
     9421 ataaaaggtt gcctgtaaaa ttacaaaaac cttacgcagc gtcaagcgga atcgtgtcaa
     9481 tcattgcgac aaatcaatcc tgtgcctaag cattacgcgg ctgactcgcc tcactcttcc
     9541 tttgggcttg agaccacatg gggtcggcgc cccgaatttt aaagggcaat tacgataaaa
     9601 atgtgatttt catcacgatt taggtgcagc catttcgtcg agatcaagtt taggacaaaa
     9661 ttttgccagt tggtgttaat gttttgttag aatcagtcac gtaatgtgag cacgattaaa
     9721 gtgagatgtg agtaaatggc gaataaacct tcggcagaag aactgaaaaa aaatttgtcc
     9781 gagatgcagt tttacgtgac gcagaatcat gggacagaac cgccatttac gggtcgttta
     9841 ctgcataaca agcgtgacgg cgtatatcac tgtttgatct gcgatgcccc gctgtttcat
     9901 tccgaaacca agtatgattc cggctgtggc tggcccagtt tctacgaacc ggtgagtgaa
     9961 gaatccattc gttatatcaa agacttgtca catggaatgc agcgcataga aattcgttgc
    10021 ggtaactgtg atgcccatct ggggcatgtc tttcccgacg ggccgcaacc aacaggcgaa
    10081 cgttattgtg ttaactctgc ttctttacgc ttcactgatg gcgaaaacgg cgaagaaatc
    10141 aacggttgaa aaaacgattc agcaacttat tccacaggag cggaaaaatt atgaatcttg
    10201 atgacattat caacagcatg acgcctgagg tataccagcg tttgtcgacc gccgttgaac
    10261 tggggaaatg gcctgatggc gttgcgttga ccgaggaaca aaaagagaat tgcctgcaac
    10321 tggtgatgct gtggcaagcc cgccataata ccgaagcaca gcatatgaca attgacacaa
    10381 atggtcagat ggtcatgaag agcaaacagc agttaaaaga agattttggt atctcagcaa
    10441 agcctattgc catgtttaag taagtggttc atcccctttt tgccgcggct atgaagggga
    10501 tagccaacaa tgaccccacg actcgtctca attatctcga cccgacctgg ctaagtccca
    10561 gtccgatctc ttgcagtcgc gttcacgcct gaatgacttc tcctgaactt aagtctcaga
    10621 cctatttggc cggtaatccc tctcgaattt gctctttttg cgtcccgcct tccaccaacc
    10681 tgtaacagac ccagccattt tctgcgtaat tgtgatctaa gttgtaaaat gcaggtcatt
    10741 atctgtgttg tgcagattta atgttgacct caaaaatcat gtgggtaagt atgatggcca
    10801 tctaagcaaa acaaaacaca gaggatgacg caatgaaagc actggctcgg tttggcaagg
    10861 cctttggcgg ctacaagatg attcatgtcc cacaacccat ttgtggcccg gaagacgtag
    10921 tgattgaaat taaagccgcg gcaatctgcg gcgcagacat gaagcactac aatgtcgata
    10981 gcggttctga tgagtttaac tctatccgcg gccatgagtt cgcaggttgt attgcgcagg
    11041 ttggtgaaaa agtcaaagac tggaaagtgg ggcaacgcgt cgtgtcggat aacagcggtc
    11101 acgtttgcgg cgtttgtccg gcctgtgaac agggtgattt tctgtgttgt acagaaaaag
    11161 taaaccttgg tctggataac aatacctggg gcggtggttt ttccaaatat tgtctggttc
    11221 ctggtgaaat tctcaaaatt catcgtcatg cgttgtggga aatccctgat ggtgttgatt
    11281 atgaggacgc agccgtactt gaccctatct gtaatgccta taaatccatc gcgcagcaat
    11341 cgaaattcct tcctggtcag gatgttgtcg tcattggcac tggcccactc gggctgtttt
    11401 ccgtacaaat ggcgcggatt atgggggcgg taaatatcgt cgtcgttggt ctgcaagaag
    11461 atgtggcggt ccgcttcccg gttgcaaaag aactgggggc gacggcagta gtaaatggtt
    11521 ctaccgaaga tgtggtggcg cgctgccagc aaatttgtgg caaagataat ctgggactgg
    11581 tgattgaatg ctccggtgcc aatatcgcac tgaaacaagc catcgaaatg ctccgcccga
    11641 atggggaagt ggtacgcgtt ggaatgggct tcaaacctct tgatttctcg attaatgaca
    11701 ttaccgcctg gaataaaagc atcattgggc atatggccta tgactccacc tcatggcgta
    11761 acgctatccg gctattagcc agcggcgcta tcaaagtcaa accgatgatc acgcatcgta
    11821 tcggcctgtc gcaatggcgc gaagggtttg atgcgatggt cgataaaacc gcaatcaaag
    11881 tgatcatgac ttacgacttt gatgaataac tactctcctg aaggaacgtt atcgcatgga
    11941 acagataaca aaaccgcatt gtggtgtacg gctggatcgc ttaccggatt gccgctggca
    12001 ttcgtcaatg tttgccatcg tcgcgtttgg cttgcttgtc tgctggagta atgccgttgg
    12061 tggcttgatc ctcgcgcagc tgaaagcgtt gggctggaca gataattcca ccacagccac
    12121 attctcagca atcacgaccg ccggaatgtt tctcggtgcg ctggtgggcg gcatcattgg
    12181 cgacaaaacc ggtcgcagaa atgcgttcat cctctatgag gccattcata ttgcctcgat
    12241 ggttatcggt gctttctcac cgaatatgga ttttcttatt gcctgccgtt ttgtgatgga
    12301 cgttgggctg ggagctttac tggttacact gtttgctggt ttcaccgaat atatgcccgg
    12361 tagaaatcgc ggaacgtggt ccagtcgggt ttcctttatt ggcaactggt catatccgct
    12421 ctgctcactg attgcgatgg gactcacgcc gctgattagt gcagagtgga actggcgggt
    12481 acaactgctt attcctgcaa tattgtcgct tatcgctacg gcgctggcct ggcgctactt
    12541 tcctgaatcc ccgcgctggc tggaatcgtg cggacggtat caggaagccg agaaagtcat
    12601 gcggagtata gaagaaggcg tcatacgcca gacgggtaaa cctttgccgc ctgtggttat
    12661 tgctgatgac ggtaaagcgc cacaagcggt gccgtattca gccttactga caggagtatt
    12721 actgaaacgc gtgatattag gctcttgtgt gctgattacc atgaacgttg tgcagtacac
    12781 actaattaac tggttgccaa caatattcat gacccagggg attaatttaa aagactcgat
    12841 tgttttaaat accatgagta tgtttggcgc gccatttggt atttttattg ccatgctggt
    12901 gatggataaa attccgcgta aaacaatggg tgtggggcta ttaatcctga ttgcggtgct
    12961 cggatatatc taccagtatg ttgctcataa cgctgattgg tttcttcctg attactttcg
    13021 tctatatgta cgtttgctat gcctcggcag tgtatgtccc tgaaatctgg ccgacagagg
    13081 ccaaactccg tggctccggt ctggcgaatg cggtagggcg aatcagtggt attgccgcac
    13141 cttatgccgt tgcagtgctg ctcagtagtt atggtgtaac gggagtcttt attcttctgg
    13201 gggctgtttc aattattgtc gcaattgcta tcgccaccat tggaattgaa accaaaggtg
    13261 tctccgttga aagtttaagt attgatgcag tagccaataa ataatacggg tggattcaaa
    13321 atgaaaaatt caaaagcaat attgcaggtg ccgggcacaa tgaaaattat ttcagcagaa
    13381 ataccagtgc ctaaagaaga tgaagttttg attaaagtag aatatgtcgg tatttgtggt
    13441 tcagatgtac atggttttga atcaggcccg tttattccgc ctaaagaccc aaatcaagaa
    13501 attggcctgg gtcatgaatg cgccgggacg gttgtggctg tgggaagccg cgtgcgcaaa
    13561 tttaaaccgg gggatcgggt aaatatcgaa cctggcgttc cttgcggtca ctgtcgttac
    13621 tgtctggaag gcaaatataa catctgcccg gacgttgatt ttatggcgac acaacccaac
    13681 taccgcggcg cattaacgca ctatctgtgt catccggaga gctttactta caaactgccc
    13741 gacaatatgg acacgatgga aggggcgctg gtggagcctg ccgcagtcgg gatgcatgcc
    13801 gcgatgctgg cagatgttaa accgggtaag aagataatta ttctgggagc aggttgtatt
    13861 ggtttgatga cgttgcaagc gtgcaaatgc ctgggcgcaa cggaaattgc cgtcgttgat
    13921 gtactggaaa aacgtctggc aatgggggaa cagcttggtg cgacagtggt tattaatggc
    13981 gcaaaagagg acactattgc acgctgtcag caatttaccg aagacatggg cgcagatatt
    14041 gttttcgaaa cagcgggttc tgcggtcacc gttaaacagg cacctttaat ctggtaatgc
    14101 gcggcggtaa aattatgatt gttggtactg tacccggcga ttcggcaatc aatttcctca
    14161 aaatcaatcg cgaagtcact atccagacgg tattccgcta tgccaatcgt tatccggtca
    14221 cgattgaagc tatttcttca gggcgattcg atgtgaaatc gatggtgacg catatttacg
    14281 attatcggga tgtacaacag gcatttgaag agtcagttaa caacaaacgc gacattatta
    14341 aaggcgttat taaaattagc gattaattaa tacgggagaa cagaacatgc tcgcagatat
    14401 cagatattgg gaaaacgatg ccacaaataa acattatgca attgcccatt ttaacgtatg
    14461 gaatgcagaa atgttgatgg gcgttaaaga cgcagccgaa gaagcgaaat ccccggttat
    14521 tatttctttt ggtacaggtt ttgtcggtaa cacctcattt gaagatttct ctcacatgat
    14581 ggtatcaatg gcacaaaaag caacggtgcc ggtaataact cattgggatc atggtcggag
    14641 tatggagatt attcataacg cctggactca tggtatgaat tcattaatgc gtgatgcttc
    14701 tgcatttgat ttcgaagaaa atattcgttt aaccaaagag gctgttgatt tctttcatcc
    14761 gctgggtatt ccggtagagg cggaattagg gcatgtcggt aatgaaaccg tttatgagga
    14821 agcgctggcg ggctatcact ataccgatcc tgaccaggcg gctgaatttg ttgaacgtac
    14881 gggctgtgac tcattggctg tcgccatcgg caaccagcat ggggtttata cgtcagagcc
    14941 gcaattgaac tttgaggtcg tcaaacgcgt acgcgatgct gtttctgttc cgctggtttt
    15001 gcatggcgca tcggggatca gtgatgccga cattaaaact gcaatttcgt tgggtattgc
    15061 gaaaatcaac attcatacgg agctctgtca ggccgcaatg gtcgcagtga aagaaaacca
    15121 ggatcagcct ttcctgcatt tagaacgtga agtacgtaaa gctgtaaaag aacgggcatt
    15181 ggaaaaaatt gaactgttcg gttctgatgg caaagcggaa taatgatatg gataatctcg
    15241 acgttatttg tataggtgcc gctattgttg atattccatt acaaccggtc agtaaaaata
    15301 tctttgatgt agattcttat cctcttgaaa gaatcgcaat gagcaccggg gggcgatgca
    15361 ataaatgaag caacaattat ttctcgtctg gggcatcgta ccgcattaat gagccgcatt
    15421 ggtaaagatg cctcagggca atttattctc ggccattgtc gcaaagagaa tattgatatt
    15481 caaagcctga aacaggacgt cagcatagat acatctatta acgttggact ggtgacggag
    15541 gatggcgagc ggacgtttgt cacgaaccgt aacggcagtt tgtggaaatt aaatattgac
    15601 gatgttgatt ttgcgcggtt tcctcaggcg aaattattat cactggccag tattttcaac
    15661 agtcctctat tggatggtaa agcgctaaca gaaattttta cgcaagcgaa agcccggcag
    15721 atgatcattt gtgccgatat gatcaaaccg cggttgaatg aaacgctgga tgatatttgc
    15781 gaagcattga gttatgtcga ttatctgttt ccaaattttg ccgaggcaaa attactcaca
    15841 gggaaagaga cactggatga aattgctgac tgctttcttg cgtgcggcgt aaaaacggtg
    15901 gtgattaaaa cgggtaaaga cggctgcttt atcaagcgtg gtgacatgac gatgaaggtg
    15961 ccggcggtcg caggaataac cgccatcgac accattggcg cgggcgataa ctttgcttca
    16021 ggttttattg cggcactgtt agaaggcaaa aatctgcgtg aatgcgcacg ctttgccaat
    16081 gcaacggcgg ctatctcggt tctaagcgtc ggtgccacca ccggcgtaaa aaacagaaag
    16141 ctggtcgaac aattgctgga agaatacgaa ggataatgaa ggcaaatgaa aaagatacct
    16201 ttaggcacaa cggatataac gctttcgcga atggggttgg ggacatgggc cattggcggc
    16261 ggtcctgcat ggaatggcga tctcgatcgg caaatatgta ttgatacgat tcttgaagcc
    16321 catcgttgtg gcattaatct gattgatcct gcgctaggat ataactttgg caatagtgaa
    16381 gttatcgtcg gtcaggcgtt aaaaaaactg ccccgtgaac aggttgtagt agaaaccaaa
    16441 tgcggcattg tctgggaacg aaaaggaagt ttattcaaca aagttggcga tcggcagttg
    16501 tataaaaacc tttccccgga atctatccgc gaagaggtag aagccagctt gcaacgtctg
    16561 ggtattgatt acatcgatat ctatatgacg cactggcagt cggtgccgcc attttttacg
    16621 ccgatagctg aaactgtcgc agtgcttaat gagttaaagg ctgaagggaa aattcgcgct
    16681 ataggcgctg ctaacgtcga tgctgaccat atccgcgagt atctgcaata tggtgaactg
    16741 gatattattc aggcgaaata cagtattctc gaccgggcaa tggaaaacga actgctgccg
    16801 ctatgtcgtg ataatggcat tgtggttcag gtttattccc cgctagagca gggattgttg
    16861 accggcacca tcactcgtga ttacgttccg ggcggcgctc gggcaaataa agtctggttc
    16921 cagcgtgaaa acatgctgaa agtgattgat atgcttgaac agtggcagcc actttgtgct
    16981 cgttatcagt gcacaattcc cactctggca ctggcgtgga tattaaaaca gagtgattta
    17041 atctccattc ttagtggggc tactgcaccg gaacaggtac gcgaaaatgt cgcggcactg
    17101 aatatcaact tatcggatgc agacgcaaca ttgatgaggg aaatggcaga ggccctggag
    17161 cgttaaatac tcaacacgat tcacctctgt ttctgtgaaa actgtgatga aaatctgtga
    17221 ttcaaatagg ttattttgaa agtaaacatc ggttatgcga taatcgcgct aatgtgatgt
    17281 aaaaagatac aggggtgtaa tcgtggcggc aaaagacagg attcaggcaa ttaagcaaat
    17341 ggttgccaac gataaaaagg tgacagtctc aaatttgagt gggatttttc aggtaaccga
    17401 agaaaccatt cgccgcgatc ttgagaagct ggaagatgaa ggctttttga ccagaaccta
    17461 tggtggtgct gttttaaata cagcggtgtt gacggagaat atccattttt ataagcgcgc
    17521 ttcatcgttt tatgaagaga agcagcttat tgcacgcaag gcactaccct ttatcgacaa
    17581 taaaaccacc atggcagccg attccagtag taccgtaatg gaattgctca aattgttaca
    17641 ggaccgtagt gacctgacgt tgctaaccaa ctcggcggaa gcgattcatg ttctggctca
    17701 gtcagaaatt aaagtcgttt caacgggtgg ggaactaaac aaaaatacac tttccctgca
    17761 aggaagaatt actaaagaga tcatcagccg ctaccatgtc gatatcatgg taatgagttg
    17821 taaaggtctt gatattaaca gcggcgcgct ggactctaat gaagcggaag ctgaaatcaa
    17881 aaagacaatg atccgtcagg cgacagaagt tgcgttattg gttgatcact ctaagtttga
    17941 tcgtaaagct tttgtccagt tagctgactt tagtcatatt aattatataa taactgataa
    18001 atcaccgggt gcagagtgga tagcattttg caaagacaat aatatccagc tggtgtggta
    18061 atttaagcac acgttgtcaa tattatttgt tcctgcgcct ggaaataaaa tgatgataaa
    18121 gatgcggcaa gtgaatttgc cgtaattgct accttttata gcaaatccga ggcacccaat
    18181 ggaacaatat gatcaaattg gcgcaagact ggaccgcttg cctttggccc ggtttcatta
    18241 tcgtatattt ggtataataa gctttagtct gttattaacg gggtttttga gttactcagg
    18301 taatgtcgtc ttagcaaagc tggtaagcaa tggatggtca aataatttcc tcaatgccgc
    18361 ctttacctcg gcattaatgt ttggttattt catcggctca cttactggtg ggtttattgg
    18421 tgactacttt gggcggcgca gggcgtttcg cataaatctt ctcatcgtcg gtattgctgc
    18481 aacaggggcc gcttttgtcc ctgatatgta ctggctcatt ttcttgcgct tcctgatggg
    18541 aacaggaatg ggggcgctga ttatggttgg ctatgcctca tttacggagt ttatccccgc
    18601 gacggtgcgt ggaaaatggt ccgcgcggct ctcatttgtt ggtaactggt cgcccatgct
    18661 gtctgcggcg ataggcgtgg tggttatcgc tttttttagt tggcgaataa tgtttcttct
    18721 gggtggtatt ggcatactgt tagcctggtt tctctcaggt aaatacttta tcgagtcgcc
    18781 acgatggctg gcagggaaag ggcaaatcgc cggtgcagaa agccaacttc gtgaagtaga
    18841 gcagcaaatt gaaagggaga agagtattcg tttaccccag cttactttga accagagcaa
    18901 cagcaaggtt aaggtaatca agggtacctt caggctcctg tttaaagggg aaatgttacg
    18961 acgtacatta gttgcgataa ctgttttaat tgcaatgaac atttcgcttt ataccattac
    19021 cgtatggata ccgaccatat ttgttaactc cggcattgat gtcgataaat caatattaat
    19081 gaccgctgtt attatgattg gtgctccggt aggaatattt attgcggcat taattattga
    19141 tcattttcct cgtcgattat ttggctccgc cttacttatt attattgccg tgttaggcta
    19201 tatctattca agtcagacta cagagtgggc gattttaatc tatggtctgg tgatgatctt
    19261 ctttttatac atgtatgttt gcttcgcgtc ggcggtttat atcccggagc tttggccaac
    19321 gcatttacgc ctgcgcggtt cgggtttcgt taatgccgtc ggacggatcg tcgcagtttt
    19381 cacgccctat ggcgttgcgg cattattaac acattatggg tcgatcacgg tgtttatggt
    19441 gcttggtgtc atgttagtgc tctgtgcgct ggttctctcc atttttggca tcgaaacgcg
    19501 gaaggtgtcg ctggaagaga tttctgaggt gaattaaatc tttcgttgat ggcatcaatt
    19561 gccagatgta ccactgacgt gttttatttg gcctacaatg ccagtacaga attgtaggcc
    19621 agcgtaaaat tacccctgcg tctcttccca gtctgccagc gtatatagcg ttgccccagc
    19681 tgctgacatc tccataaacg cgtgtgcact gtcctggggc tggatattca cgccacgaca
    19741 gccatcggta atcacgttta ccttataacc taactgtaac gcgtccagca cggtaaactt
    19801 cacgcaatag tcagtagcca ggcccataac gatcaattca tcgatttcat gatcgcgcaa
    19861 ccagtcatca agcgcggttt tctgccgacg tccgttatca aaaaaggcac tgtaactgtc
    19921 aactaaagga ttttcgcctt tatggaacac cgctgcgatc tctttttgat tcagtaacgg
    19981 atgtaattgt gcgccttcac tgttctgcac acagtgttca ggccagaaag tttgccgcag
    20041 gccgtcgagt tggcctggcg tataaggctc tacgccgtgc tggctggcaa agctgccgtg
    20101 attcgccgga tgccagtcct ggctggcgat aaccgcttct ccgcgcgact ggcaccagtc
    20161 aataaggcgg ttagcaacct ccaccgtact gtcaccttcc ggtacggcga gcgcgccacc
    20221 agcacagaaa tcattttgta aatcgaccag taacagggcg cgatggggca ttgcagtctc
    20281 cttaatcatc cggcgtcagt tcgccgcgca ggttttggct catggccttg cgaatggttt
    20341 cagtatccag ttcctggctc agcaggtaat gcagtttggt tagcgtggct tctacagtca
    20401 tatctgcacc gccaataacg ccggcatggg cgaggacgtt accggtggca taaccactca
    20461 tattcacttt accggacata cattgtgtca ggttgaccac cacaataccg cgatcgctgg
    20521 cttcttgtaa ttcctgcagg aaggctttgt tttgtggcgc attacccacg ccataggagc
    20581 gcagaatcaa tgctttcacc ggttggcgca gaaaattgcg cacgacgtca gcagaaatcc
    20641 ctggataaat cgtcactacg ccaattggtt gtggcgtgat tggatgaacg attaattccc
    20701 cttcaccgtg cggggcgggt ggcgtattca aacgacgaat atggatacca gcttccagta
    20761 acggaggaag gtttggagag gcaaacgcat caaaaccatc ggcatgggct ttggtagtgc
    20821 ggttgccgcg atacaatcgg ttattgaaaa agagcgttac ttcgttgatc ggataattcg
    20881 ccgcaacgta caacgcattc agcagattaa tttgtccgtc agagcgtaac tcagccagcg
    20941 ggatttgtga ccctgtcaca atgaccggtt taccgagatt ctcgagcatg aacgacagcg
    21001 cagaggcggt atacgccatc gtgtcggtac cgtgtagaat gacaaaacca tcatagtcgt
    21061 catagtgcgc tttaatatct tcagcaatat gctgccagtc ttccggcgtc atatcagaag
    21121 aatccatcaa cggcgtatat tcatgaatgg tgaaatctgg catctccagg cgatggaatt
    21181 ccggcatcag cgccagttgg cgttgtagat gacctgacac cggaatataa ccctgctcgg
    21241 aacgctgcat cccgatggtc ccgcccgtgt aggcgacgta aattgatttc ttttgcatga
    21301 tattgatatg aatgatatcg aaagagcaaa agtatatacg tgaggcagga ggagcgcacc
    21361 tgatactatg cggataagga aatatggggc gagacagcgg gaagaggatt cgtcgaggta
    21421 aaaaataatg tggccgacga tctgtcggcc actcagtaca agacttaacg cacgttggcg
    21481 caggtcaggc aaaacgcata acggttttgt gggtcgttaa acgcggccag cttgtcgctt
    21541 tcacttttaa cggtagaggc taccgaggca agcggtgcag gtaacatggc ctggaacgca
    21601 tctggcaaca ttgcccggac agaaccagac atgttgtcca tcactttgtc gaagaaggtc
    21661 ggttcatcaa cgtagtattc cagatgccac tgtttcactt ttgcgagctc tgctgctttg
    21721 gcaaccgcat catcgaaatc cccgagacta tcgaccagcc cgttagcttt tgcatcctga
    21781 ccggtccaga cgtggccctg ggcgatttta tcaatttgct ccggcgtcga atgacgcgca
    21841 tcagcaacca gcgtgataaa gcgtttatag ccattctcaa tgcttaattg catcatctgc
    21901 tgcgcttccg gcggcagtgc cctggtgata gaaacatccg ccagcggtga agttgagaca
    21961 ccatcagtat gaacaccaat cgaatccaga ctattttcta cggtggtgat cacgccgaag
    22021 ataccgatag aaccggtcag ggtgctgggg ttagccacaa tgtaattagc tggcgtggaa
    22081 atccagtaac caccagatgc cgccatgccg cccatcgata caaccacaga cttacccgct
    22141 gcccgggctg ctgccagttc agcgcgaatc acttcagacg cggtaacgct gccgcctggg
    22201 ctattaacac gcaggacaat cgctttcact ttcgggtcaa ggcgagcgtc gcggatttgt
    22261 gccgcagtgg tatcaccgcc aacattcccc tgagtttcct cgccatccat aattgcgcca
    22321 ttagcgaaga cgacaccgat gctgtcaccg gtatctgccg gcgttttcaa tgcgtaatcg
    22381 taataactga tggcgcgata atttttatca gtcttactcc agccgaactc tttggtcaga
    22441 gctttttcga tttcggcact cgatgccagt gcatcgacca gcttgttttc cagtgcatat
    22501 ttcgcggtat cgccaccggt tttggttaaa ccctcaagca acccttgcgc gccagggaat
    22561 acctgctgag cagggatctg ccggttagcg gcaacagtat tcagatagtt ttgccacagc
    22621 tcaccaatcc agcggctgtc agcttcgcgg gctgccggtg acatatcatc acgaataaac
    22681 ggttcaacgg cagatttata cgtacccacg cggaacacat gggtggaaac tttcagctta
    22741 tccagcaacg atttgtagta cagaccgttg gtagcaaaac cgtgcagatc aaccacgcct
    22801 tgcggtgaca gccaaatttt attggcgaaa ctggcgagat aatattgccc ctggctgtag
    22861 ttctcgccaa cggcataaac cggtttcccg ctgtcacgaa actctttcag ggctttgccg
    22921 atgtactgca tagacggctg gtcgccgcct gcgaagtttt tcagatccat cacgataccg
    22981 gtgatattgc ggtcgtcctt cgcctggcga atggtgttga cgatatcaaa cagtgagttt
    23041 tcctgcagac gatcggaact ggcaccaagc agctggcggc ttaatttact aaaccgctga
    23101 gaactgtctg gtttatcgac gatcacacca gaaatgtcca gcagcagtgc gccacgactg
    23161 gccgtttctt tcgaatcacc accactgacc tgcatccaga tccccacacc aaccagtacg
    23221 aggaaaataa agaacaggtt aagtaccatt tcacggacga aattcagcag acgccacgtc
    23281 catttaaaaa atccggcaat aaatcgccaa agggttcgca tgtattctcc caacttaagg
    23341 tcacacctgt cacaggcgca atagatgtgg ttatcgtaat gacccaaccg gcacttgtca
    23401 gcaggaattg cggactgtgc tgtaacaaaa tccactctcg tgttaatttt gtgaataaat
    23461 atcacgacag gagttaatca aatggatgca ctcgaactat tgatcaatcg ccgtagcgcc
    23521 tcccgcttgg ctgaacccgc gccaacgggt gaacaactgc aaaacatcct gcgtgcgggt
    23581 atgcgtgcgc cggaccataa gtccatgcaa ccgtggcatt tttttgtgat tgaaggggaa
    23641 gggtgcgagc gtttcagcgc cgtactggaa cagggggcga ttgctgccgg tagtgatgat
    23701 aaagctatcg acaaagcccg taatgcgccg ttccgcgcac cgctcatcat cacggtggtg
    23761 gcgaaatgca aagaaaatca taaagtcccg cgatgggaac aggaaatgtc tgccggatgc
    23821 gcggtcatgg cgatgcaaat ggcagccgtt gcccagggat ttggcggcat ctggcgcagt
    23881 ggcgcattaa ctgaaagtcc ggtagtgcgt gaagcattcg gttgccgtga gcaggataaa
    23941 attgtcggtt ttctctacct cggcacgccg cagttgaaag catctacgtc gattaacgtc
    24001 ccggacccga cgccgtttgt aacttatttc tgataattgt cgcgaaactg tctggattgt
    24061 gagcaaaggc gcaggaattc agacactctc acttatcact tcacggaatg agggttacca
    24121 tacccggatt gcattgacag gagatgtcca tgagcgagaa ctcgattcgt ttgacccaat
    24181 acagccacgg agctggttgc ggctgtaaaa tttccccaaa agtgttggaa accatcctgc
    24241 acagtgagca ggcgaagttt gttgatccga atttgcttgt gggtaatgaa acccgcgacg
    24301 atgcggcggt gtacgatctg ggcaatggca ccagcgttat cagtaccacc gacttcttta
    24361 tgccgatcgt tgataatcct ttcgattttg gccgcattgc ggcgactaac gccatcagcg
    24421 atatcttcgc gatggggggc aaaccgatta tggcgattgc gatcctcggc tggccgatta
    24481 acaaactttc cccggaaatt gcccgcgaag taaccgaagg tggacgctat gcatgccgtc
    24541 aggcgggtat tgcgctggct ggcggtcact ccatcgatgc gccggagccg atttttggtc
    24601 tggcggtaac ggggatcgta ccgaccgagc gggtgaagaa aaacagtacc gcacaagccg
    24661 gatgcaaact gttcctgacg aaaccgctgg ggatcggcgt tcttaccacg gctgagaaaa
    24721 aatcactgtt gaaaccagaa catcagggac tggcgacgga agtgatgtgc cggatgaaca
    24781 tcgcaggcgc gtcctttgcc aacatcgaag gcgtaaaagc gatgaccgac gttacgggct
    24841 ttggtctgct gggccacttg agcaaaatgt gtcagggggc tggtgtgcag gcacgcgtcg
    24901 actatgaagc gatcccgaaa ctccccggtg ttgaagagta cattaagttg ggcgcagtac
    24961 ctggcggcac tgaacgtaac tttgccagct acggtcatct gatgggtgaa atgccgcgtg
    25021 aagtgcgcga tctgctgtgt gatccgcaaa cttctggcgg tttgctgctg gcggtcatgc
    25081 cggaagcaga aaatgaggtt aaaactacag ccgccgagtt tggcattgaa ctgacggcga
    25141 ttggcgaact ggtgccagcg cgcggcggtc gtgccatggt tgagatccgt taattcaatg
    25201 cggttgttta ttgccgaaaa accgagtctg gcgcgcgcca ttgctgatgt cctgcccaaa
    25261 ccgcaccgga aaggcgatgg ctttatcgag tgcggtaatg gtcaggtggt gacttggtgt
    25321 atcggtcacc tgcttgagca ggcgcagcca gacgcctacg acagccgcta tgcgcgctgg
    25381 aatcttgcgg atttgccgat tgtcccggaa aagtggcaat tacagccacg accctccgtg
    25441 accaaacaac ttaacgtcat caaacgcttc ctgcatgaag ccagcgaaat cgttcacgcc
    25501 ggggacccgg atcgtgaagg gcaattgctg gtggatgaag tgctggacta tctgcaactg
    25561 gcaccggaaa agcgccagca ggtgcagcgt tgcttgataa acgacctgaa cccgcaggcg
    25621 gttgagcggg cgatcgaccg tcttcgttcc aacagtgagt ttgtgccatt gtgcgtttct
    25681 gcgctggcgc gagcgcgtgc cgactggctg tacggcatca atatgacccg tgcgtatacc
    25741 attctcggtc gcaatgccgg ttatcagggc gtactttccg tgggacgcgt gcagacaccc
    25801 gtgctcgggc tggtggtgcg ccgcgatgaa gagattgaaa acttcgtggc gaaagacttc
    25861 tttgaagtca aagcacatat cgtgacacct gccgatgagc ggtttaccgc tatctggcaa
    25921 ccgagcgaag cgtgtgaacc gtaccaggat gaagaagggc gcttgttaca tcgtccactg
    25981 gcggagcatg tggttaaccg cattagtggt caaccggcta ttgtcaccag ctataacgat
    26041 aaacgggaat cagaatccgc gccgctgccg ttttcgcttt cggcgttgca gattgaagcg
    26101 gcaaaacgct tcggtctgag cgcgcagaac gtgcttgata tctgccagaa gctgtacgaa
    26161 acgcacaagc taatcactta tccgcgttcc gattgtcgct atttgccaga agaacatttt
    26221 gccggacgcc acgcggtgat gaatgccatc agcgttcatg caccggatct gttgccgcag
    26281 ccagtggtag atccagatat acgcaaccgc tgttgggatg acaaaaaggt cgatgcgcac
    26341 cacgccatca tcccgaccgc acggagttct gcgatcaacc tgacggagaa cgaagcgaag
    26401 gtctataacc tgattgcccg tcaatacctg atgcagttct gcccggatgc ggtgttccgc
    26461 aagtgtgtta tcgaacagga cattgccaaa ggcaaatttg tcgctaaagc ccgttttctt
    26521 gctgaagcag gctggcgcac gctgttaggc agcaaagagc gcgatgaaga aaacgacggc
    26581 acgccattgc ctgtggtggc gaaaggcgat gagttgctgt gtgaaaaagg tgaagtggta
    26641 gagcggcaaa cccagccgcc gcgccatttt accgatgcaa cactgctttc ggcgatgacc
    26701 gggatcgcgc gctttgtgca ggacaaagat ctgaaaaagg tccttcgtgc gaccgatggt
    26761 ctggggacag aagcaacccg tgccgggatt attgaactgc tgttcaagcg tggttttctg
    26821 accaaaaaag ggcgctatat ccactccacc gacgccggaa aagcgttatt ccattcgctg
    26881 ccagaaatgg cgacgcgacc ggacatgacc gcgcactggg aatcggtgct gacgcaaatc
    26941 agcgaaaagc agtgtcgcta tcaggacttt atgcagccgc tggtggggac gctatatcag
    27001 cttattgatc aagccaaacg tacgccggtg cggcagtttc gcggcattgt ggctccgggc
    27061 agtggtggca gtgctgataa gaaaaaggct gcaccgcgta aacgtagtgc gaaaaaaagt
    27121 ccgccagcag atgaagccgg aagcggggcg atagcgtaag cgagcgaatc tttcgtgcta
    27181 ttcgagtcat attctgaaat atccagcgga tcaagaaaat tcgttggata tttttttgca
    27241 tggataaaat tatcgcctct aaagtatgta ataacaggga atgtgatgaa aaaagtttta
    27301 ttacaaaacc atcctgggag cgagaagtat tcttttaatg gctggggaat atttaatagt
    27361 aattttgaac gggtgattaa agaaaataag gccatgctgc tttgtaagtg ggggttttat
    27421 ttaacatgtg ttgtcgctgt aatgtttgta ttcgcagcga taacatccaa cggtttgaat
    27481 gaaagaggcc tgattaccgc gggatgctct tttctttatc tattaattat gatgaggctt
    27541 attgttcggg ccggttttaa agcaaaaaaa gaacaactac attattatca ggctaaaggt
    27601 attgagccgc tcagtatcga gaagttacag gcgctacaat tgatcgcacc ttatcgattc
    27661 tatcataagc aatggtctga aacgctagag ttctggccgc gaaagcctga acctggcaaa
    27721 gataccttcc aatatcatgt gcttcctttt gactcgatcg atatcatcag taaaagacgc
    27781 gagtctttag agaatcaatg gggtatcgaa gatagcgaaa gttattgtgc cttaatggag
    27841 cattttcttt ctggcgacca tggagccaat acctttaaag caaacatgga ggaagcccca
    27901 gagcaggtta tcgccgtgtt gaataaattt gctgtttttc cctcagacta tatctctgat
    27961 tgcgctaata atcgctccgg taaatcctcg gcgaagctaa tatgggcggc ggaattatca
    28021 tggatgatct cgatatcaag cacagctttt caaaacggga caattgaaga agaactggcc
    28081 tggcattata taatgcttgc ttctcgaaag gcgcacgagt tgttcgaaag cgaagaagat
    28141 tatcaaaaaa atagtctaat gggatttctt tactggcata tctgctgcta tcgcagaaag
    28201 ttaacggatg ctgaacttga agcgtgttat cgttacgaca agcagttttg ggagcactac
    28261 agtaagaaat gccgttggcc cataagaaat gttccctggg gagcatcatc cgttaaatac
    28321 tcataaacgc ctgaaatttt gccgggggcg ctgcgcttgc ccggcctaca ttagagcgta
    28381 actgcaatga attgtgccca tctgtaggcc tgataagcgt agcgccatca ggcatttaca
    28441 acttaaatca caccctgcgc cagcatcgca tcggcaacct tcacaaaacc ggcaatgttc
    28501 gcgccctgca cgtagttggt ttgctcacct tcaccaccat gctcaacaca ggcatggtgg
    28561 atatccagca tgatgtgatg caaacgtgcg tcaactttct cggctttcca gcccaggcgc
    28621 gcagcgtttt gtgccatttc caggcccgac gttgctacac cgccagcatt agccgcttta
    28681 cccggtgcaa acagtacgcc tgcctgctgg aacagttcag tcgcttcgat ggtggtcggc
    28741 atatttgccc cttcagcgac ggctttaacg ccattggcga taagctgatg cgcggtgtca
    28801 acatccagtt cgttctgggt ggcgcaaggc agggcgatat caaccggtac agaccacggc
    28861 tgttggcctt cgagatagac cagaccaaat tctttggcgt aatctgccac tcgaccatcg
    28921 cggctggctt tgatttcgat aagacgtgcc agtttctctt tcgtgaatcc gctttcatca
    28981 actacagtgc cgctggagtc tgacgcagtg atcgcacgag caccaaattc catcgctttt
    29041 tcgatagcgt actgggcgac gttgccggag ccagaaacgg aaacgcgcat cccttcaaaa
    29101 cccataccgt ggcgttttaa cattgcttct gtgaaataaa ccagaccgta gccggtagct
    29161 tccgggcgaa taagactacc gccaaatgaa aggcccttac cggtgaagac gcaggcggta
    29221 ttgttggaga gctttttcat catccccgcc ataaagccga cttcacgacc accaacgccg
    29281 atatcacctg ccggaacgtc ggtatccgcg cccaggtggc gatacagttc agtcatcagc
    29341 gcctggcaaa aacgcatcac tttaccttcg ctttttcctt tcggatcgaa atcgctgccg
    29401 cctttaccac cgcccatcgg cagggtagtc agggcatttt tgaaggtttg ttcaaagccg
    29461 aggaatttga gaatggaaag gttaactgac ggatggaagc gcataccgcc tttgtacggg
    29521 ccgatggcag agctgaactg cacacgccat gcacggttga cctgtatctg gttgcgatca
    29581 tcaacccata ccacgcgaaa ctggatcacg cgctccggtt caaccagacg ctccagtaat
    29641 gacatctggc gatattttgg attttgttca agaaaaggcc agagtgtggt cattacttca
    29701 cgaacggctt gcgcgaactc ggtttgattc gggtcgcgct tttggacatg gttgaggaat
    29761 gactccagag aatatgtctg atccatagat ataaaaacct tatatattaa tacgattgtg
    29821 cttatgttgt ttatgtcatg tgcttttgca gttttcgact ataccatcaa gaatgtgacg
    29881 gtaagcaaga aaaaaatgac ccaggaaagc aaattaataa cgagagtaat ctcataacca
    29941 aatgcgataa ctcagacgct aaagttttgc cacccggcta ccgttatcag tagcagagac
    30001 aataacgtta aggagttccc tgtgagtcga gcattgttcg ccgtagtatt agcatttcca
    30061 ttaattgcac tggctaaccc gcattatcgt ccggacgtgg aggtaaacgt accgccagaa
    30121 gtgttcagtt caggcgggca gtcagcgcag ccctgtactc agtgctgcgt ttatcaggat
    30181 caaaattatt ctgaaggggc ggtaattaaa gccgaaggaa tactgctgca atgccagcgt
    30241 gatgacaaaa cccttagcac taatccgctg ctctggcggc gcgtaaagcc ataaacgcct
    30301 ctaataatgg aatgtcagca ggggccagcg gatattgcag cgcctcttca ggtgagcacc
    30361 agaccagcgc ctgatgttca tgtgcctgta acgtcccgtg gaagtcgggt acgtgccagg
    30421 catgaagatg gataatccgg cccgaaactt ctcgctgatg gctggcaaca tactcaccca
    30481 cagttgcttc gatgtccaat tcttcgttta actcacgcac cagcgcctgc tgctggcttt
    30541 catccagctc gactttacct ccggcaaact cccataatcc cgcctgatcg ctctgggcgg
    30601 ggcgttgcgc gagtaaaatt ttgccatcac gttcaatgat ggcggcaaca acttcaatca
    30661 ttttcataaa ttacacacgg gcagataagc gaaaaatgcc ataatatccg cactgttgct
    30721 caatcagaaa ggctttatat ttaaggagtg cctgtgctag accgccatct tcatccccgg
    30781 attaaaccgt tgttgcatca gtgcgtgcgg gttcttgata aatcgggcat tacgccagat
    30841 ggtttaacat tagtgggatt tgccatcggc gtgctggctc tgccgtttct ggcgctgggc
    30901 tggtatctgg cggcgctagt cgtcattttg ttgaacaggc tgcttgatgg tctggacggt
    30961 gcgctggcgc ggcgcagagg gcttaccgac gcgggcggct ttcttgatat ttctctcgat
    31021 tttctctttt acgcgctggt gccgtttggc tttattcttg ctgcaccgga gcaaaatgcg
    31081 ctggcaggcg gctggttgtt gtttgcgttt atcggcacgg gcagcagttt tctcgccttt
    31141 gctgcactgg cagcaaaaca tcagattgat aaccccggtt atgcacataa gtcgttttac
    31201 tatctggggg gattaaccga aggcacagaa acgatcttac tgtttgtgct gggatgttta
    31261 ttccctgcat ggtttgcgtg gtttgcgtgg atcttcggtg cgttgtgctg gatgacgacc
    31321 tttacgcggg tgtggagtgg gtatctgacg ctgaagtcgc tccagcgtca gtaattgcaa
    31381 tgttatttgc tactgcccgg gccgcgttca ccggttgcaa ccggattttt tggatcgctg
    31441 ctccattcgt accagccgcc gtcatacacg gaaacattct tccaacccat ggcgcgtgcg
    31501 tacataaagg tttcggacgc gcgccagccg gtgccgcagt agaatgaaac ttgctgatct
    31561 ggtttgatat tccatgcttt ccacatagcg gtaatatcat cggcgctacg catggtgcca
    31621 tccgggttat ggaaatcttc catatgcgtc gagtcgctac cagcgtgtcc ccaacgtgct
    31681 ccggctattt caccttttgg tttaatatag ctgtaaccgc tggtcgtacc gataaattct
    31741 ggccacgaac gaatgctcac cagcgatgca tcctggcgat gcagcagtcc acgggcctgt
    31801 tccatatcga gcatcaactg cggttgtgcc gggatcttca cgccgaaatc tggttccgct
    31861 ttcactttcg gtggcgttcc tcgctcaaca ggcagtcccg cgtcggacta ggtttgccag
    31921 ccgccatcca gcagacgcac atctttcacg cctgcgtaga gcataatttg cgccacacgc
    31981 gctccagcgt atacgtcacg cccgtacaga atgaccgtgg tgtcatggcg aatgccgtgt
    32041 tttgccagca tcgcttttag ttgttcatca gaaactttgt tccacagcgg ttcgctttcc
    32101 acttcgttgg tatcgatgta gtcagcgcca ggaatatggc tgataaggta aagcttagga
    32161 gcgccccagg ccgcttcaat gactttccag ccaccgacag gtttcgccgt aacctcttta
    32221 ccttgttgca ggtcatgcag ccattgcgga taaaccagct gctcaaagtg cggcagtttt
    32281 tgtagacggg aaggttcgct tagcgtgtca ctcagaatgg agatatgcgt aaaacctgct
    32341 ttttgcagtc gcgttttgac ggcgtcgaca tctttgccat taccgtacag cgccaccggg
    32401 gcatcggctt tcaggttatg ttgcttgatc cacgcgttga gctgttcggt gctcattttg
    32461 tcgagccagc tagcagagag gtttaaggcg gcaggttcat gaccagaagg gccatttaag
    32521 gtttgtggcc agccgttata aaacgcgctg gggcgagtat ctatcgcttt gccattttgt
    32581 tgctgaagtt ggtcaagtgt aagaggcttc gccagttcag cggcccacga agaagcgcaa
    32641 gccagcccta aagccattgc cagcgcggtc atttgagaaa cacgtttcat cgaaaatccc
    32701 gacattatag ttttgagtgc gccattctga actgacgcat tgagaaaaac tttgcgttat
    32761 cgcagtttgt tgtaattttc tgaccactgc gccatatcca gaacagaact atcagcagga
    32821 acatcctgga gatcgtgcgt tacctgaacg acggggatcg ccagggcgcg aacttcgctg
    32881 aacacccact ggcgaaaatt atcgcgcaga gccacatcaa gacggctgaa tggctcatcc
    32941 aggagtaacg cttttggctg ggcgagaagg gcgcgtagta gagcaacgcg cgctcgctga
    33001 ccgccagaca aagtggcagg atcctgatgg aaagctccgt caaggcctga acgctcaagt
    33061 gcatcattca cggcattacg tcgggcattc cccttaagtg tcgccggtag cgccagcagt
    33121 aaattttgcc cgacgctgaa ctggtcaaat aacagtgcat cctgaaaaag aatgccaatc
    33181 tgacgctgtg cggtgggtag catgtcaatc cgttgctcat tgagccatag ctcacctgta
    33241 caagaaaact gtccggccag tgcaccaatc atccatgaaa acagagtgga ttttccacag
    33301 ccagatggcc ccattaacgt gacgatgtca cctttatcaa ccgtaaagtt aacgtttgtc
    33361 agcaagcggc tttctggtaa acgtagcgaa acatttttca cgcagagcat tagcggattc
    33421 cttgtctgac ataacctacc cattttgcga ctaacgcggt cagggcaaaa ataataagcg
    33481 gtaacaacag ttgccataaa gcctgggcgg cgagaatacc gttgctgccg ccgctgctta
    33541 atgccaccgc ctcagtggtg agcgtcggaa aacgccccgc gcccagccac agcgtcggca
    33601 tatactgcgc aatactgact gaaaatccca ccgcgaaggc aatcagcaca gggcgcaaca
    33661 tgagtgggca tttcacgtaa aagaagattt tggcccgcga ccagcccagt gtttgcgcaa
    33721 tcaaaattaa ccgtgaatca atgcgctgcc aggcgggttg caggataaac agcatccacg
    33781 gcattaccca cagcagatgc ccccagacca ccgccgtcca gcttccatcc aggttcagcc
    33841 atagcgccag cgtgtactgg cccgccacca gtggcagagc gggcaataga atgggcaacc
    33901 atagccacaa ctggcgacgc tgtgcccccc attccagcca cagcagtaac aggagcaaag
    33961 cgatgaatgt tgctaccagc cccattgtca gactgttgat gagtgcttca ctattgatcg
    34021 tcgactgatc cgcgaggatc gccagcagaa ccacacacag cacaccggtt aagggtaaaa
    34081 aactcgccag cgtagtgcct ggtaataaag gtgtggcagg cttgcgaatg ccatctacgc
    34141 ggggaatagt gcgccgccag ctgcgccata gcagatagct cagcaaaacg taggcggcga
    34201 gtaacagcat cagcagcaga ctggcgagtg cgcctttcgt ttgttgatcc gcgtcgccct
    34261 gagttaacca ctgccagcta attactgcca gcgtcggtgg attacctggc ccaagaataa
    34321 tcgccacatc cacgaccgaa agcgaccagg caacaatcgc cagcatcgcc attgccagcg
    34381 aaggcgcgac ggagggcaac agcagccagt tcaggcattg ccagcggctg tagcccagcg
    34441 aatccagcac aatgacctgc tgcaacagcc gtttttcgct caatactgcc actaagatcc
    34501 acagcagaaa tgcgctttct ttcactgcaa gggtgaggcc cagaccgatc caaatcggtc
    34561 cattggcgga gtgaaatacg ggaaatagtc atatagcagc cctccgtcag caaagagcag
    34621 cagggcgctg gtggcaaaag ccacatgggg aatggcgagc agccacggca gacgggcgca
    34681 catacgctgc catttcggcc caggccatag cgccacaatc accagcaggg caatcaacaa
    34741 tgccccgacc gccacgatgg ttgtcgacac cagcgttgcc agtaatgcct gcggtaactg
    34801 tggatcggca aataacgcct gccagcgtgt caacgacaac gcaggcgata tcagcgtgag
    34861 agctgccggg atcagcggtg cataaatcac cgccaccatc gcccacagca aaaagattaa
    34921 tgcgtaccgt aacggcgtaa ccattcttgt tccagtgcat tcacccatcc tgcgtgcggt
    34981 tcagccagta ccggcggcag atcctgcggc attcttgatt gcaatatttc gcgctgcccg
    35041 tcaggcagtt tttgcggatc gagaacagaa ggatcgcccc agacaacggg atctgcttta
    35101 cgcagttgcg catcgggtga gagcaggaaa ttggcaacta ccttcgccgc agcactggca
    35161 ttcgcgttgg ctggaatggt gacgaaatgc acgttgccaa tcatcccctc gcgaaagcca
    35221 aaactgtaac tgcttgccgg caaatcgccg ctggcgattt tttgctgcgc atgcgcgggg
    35281 ttaaaggtca gcgacaggcg caatgtgccg gctttcagca gagcatccat ccgcgcgggc
    35341 gaaggcggga aatcctttcc ttcgcgccac aaatacggat gcagtgcatc aagatattgc
    35401 cacaagggcg cagtgacacg ggcgaaagtc gcatcgtccg gcgcttcttt taatgcggcg
    35461 ggatctggcg tcagcataat cagcaactgt tcaagaaacg ccgtgccggt aaagtccggt
    35521 gggcgcggat aagtaaccgt gccgggattg gctttagcaa actccagtaa ggcttgcggc
    35581 gtttgtggtg gctgtggtaa acgtcagttg tgcgccgccc cagggcgatt ccgcaccttg
    35641 tgtcggcact gaaaaatctt cccgcaccgg cagctgtgtg tcgacatagc gccagttggg
    35701 cagagtctcc gcccagcccg tttgtaataa attggcctct ttta
//
LOCUS       DAETPZ010000034        35407 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.034, whole genome shotgun sequence.
ACCESSION   DAETPZ010000034 DAETPZ010000000
VERSION     DAETPZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.034"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..126
                     /locus_tag="K0W59_001908"
     CDS             <1..126
                     /locus_tag="K0W59_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3022957.1"
                     /translation="RHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(414..1442)
                     /gene="epmB"
                     /locus_tag="K0W59_001909"
     CDS             complement(414..1442)
                     /gene="epmB"
                     /locus_tag="K0W59_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBI3022958.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1484..2050
                     /gene="efp"
                     /locus_tag="K0W59_001910"
     CDS             1484..2050
                     /gene="efp"
                     /locus_tag="K0W59_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBI3022959.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            2102..2227
                     /gene="ecnA"
                     /locus_tag="K0W59_001911"
     CDS             2102..2227
                     /gene="ecnA"
                     /locus_tag="K0W59_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBI3022960.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            2338..2484
                     /gene="ecnB"
                     /locus_tag="K0W59_001912"
     CDS             2338..2484
                     /gene="ecnB"
                     /locus_tag="K0W59_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBI3022961.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2660..2889
                     /locus_tag="K0W59_001913"
                     /pseudo
     CDS             2660..2889
                     /locus_tag="K0W59_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(2886..3419)
                     /gene="blc"
                     /locus_tag="K0W59_001914"
     CDS             complement(2886..3419)
                     /gene="blc"
                     /locus_tag="K0W59_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBI3022962.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3508..4641)
                     /locus_tag="K0W59_001915"
     CDS             complement(3508..4641)
                     /locus_tag="K0W59_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBI3022963.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            complement(4704..5063)
                     /gene="frdD"
                     /locus_tag="K0W59_001916"
     CDS             complement(4704..5063)
                     /gene="frdD"
                     /locus_tag="K0W59_001916"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBI3022964.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(5074..5469)
                     /gene="frdC"
                     /locus_tag="K0W59_001917"
     CDS             complement(5074..5469)
                     /gene="frdC"
                     /locus_tag="K0W59_001917"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBI3022965.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5480..6214)
                     /gene="frdB"
                     /locus_tag="K0W59_001918"
     CDS             complement(5480..6214)
                     /gene="frdB"
                     /locus_tag="K0W59_001918"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBI3022966.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDSMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(6207..8015)
                     /gene="frdA"
                     /locus_tag="K0W59_001919"
     CDS             complement(6207..8015)
                     /gene="frdA"
                     /locus_tag="K0W59_001919"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBI3022967.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            8340..9317
                     /gene="epmA"
                     /locus_tag="K0W59_001920"
     CDS             8340..9317
                     /gene="epmA"
                     /locus_tag="K0W59_001920"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBI3022968.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEANIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9536..11038
                     /gene="yjeM"
                     /locus_tag="K0W59_001921"
     CDS             9536..11038
                     /gene="yjeM"
                     /locus_tag="K0W59_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HBI3022969.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(11189..14500)
                     /gene="mscM"
                     /locus_tag="K0W59_001922"
     CDS             complement(11189..14500)
                     /gene="mscM"
                     /locus_tag="K0W59_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HBI3022970.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQGLRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALRSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     AQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(14522..15490)
                     /gene="psd"
                     /locus_tag="K0W59_001923"
     CDS             complement(14522..15490)
                     /gene="psd"
                     /locus_tag="K0W59_001923"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HBI3022971.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDARPLVDDKKDQV"
     gene            complement(15587..16639)
                     /gene="rsgA"
                     /locus_tag="K0W59_001924"
     CDS             complement(15587..16639)
                     /gene="rsgA"
                     /locus_tag="K0W59_001924"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HBI3022972.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            16734..17279
                     /gene="orn"
                     /locus_tag="K0W59_001925"
     CDS             16734..17279
                     /gene="orn"
                     /locus_tag="K0W59_001925"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HBI3022973.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            17490..17565
                     /locus_tag="K0W59_001926"
     tRNA            17490..17565
                     /locus_tag="K0W59_001926"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17523..17525,aa:Gly,seq:gcc)
     gene            17723..17798
                     /locus_tag="K0W59_001927"
     tRNA            17723..17798
                     /locus_tag="K0W59_001927"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17756..17758,aa:Gly,seq:gcc)
     gene            17834..17909
                     /locus_tag="K0W59_001928"
     tRNA            17834..17909
                     /locus_tag="K0W59_001928"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17867..17869,aa:Gly,seq:gcc)
     gene            17945..18020
                     /locus_tag="K0W59_001929"
     tRNA            17945..18020
                     /locus_tag="K0W59_001929"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17978..17980,aa:Gly,seq:gcc)
     gene            18253..18306
                     /locus_tag="K0W59_001930"
     CDS             18253..18306
                     /locus_tag="K0W59_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3022974.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(18289..19428)
                     /gene="queG"
                     /locus_tag="K0W59_001931"
     CDS             complement(18289..19428)
                     /gene="queG"
                     /locus_tag="K0W59_001931"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBI3022975.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            19427..20974
                     /gene="nnr"
                     /locus_tag="K0W59_001932"
     CDS             19427..20974
                     /gene="nnr"
                     /locus_tag="K0W59_001932"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBI3022976.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            20946..21407
                     /gene="tsaE"
                     /locus_tag="K0W59_001933"
     CDS             20946..21407
                     /gene="tsaE"
                     /locus_tag="K0W59_001933"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBI3022977.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            21426..22763
                     /gene="amiB"
                     /locus_tag="K0W59_001934"
     CDS             21426..22763
                     /gene="amiB"
                     /locus_tag="K0W59_001934"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HBI3022978.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVLVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            22773..24620
                     /gene="mutL"
                     /locus_tag="K0W59_001935"
     CDS             22773..24620
                     /gene="mutL"
                     /locus_tag="K0W59_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBI3022979.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            24613..25563
                     /gene="miaA"
                     /locus_tag="K0W59_001936"
     CDS             24613..25563
                     /gene="miaA"
                     /locus_tag="K0W59_001936"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBI3022980.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQVVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            25649..25957
                     /gene="hfq"
                     /locus_tag="K0W59_001937"
     CDS             25649..25957
                     /gene="hfq"
                     /locus_tag="K0W59_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBI3022981.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            26033..27313
                     /gene="hflX"
                     /locus_tag="K0W59_001938"
     CDS             26033..27313
                     /gene="hflX"
                     /locus_tag="K0W59_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBI3022982.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            27399..28658
                     /gene="hflK"
                     /locus_tag="K0W59_001939"
     CDS             27399..28658
                     /gene="hflK"
                     /locus_tag="K0W59_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HBI3022983.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            28661..29665
                     /gene="hflC"
                     /locus_tag="K0W59_001940"
     CDS             28661..29665
                     /gene="hflC"
                     /locus_tag="K0W59_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HBI3022984.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            29747..29944
                     /locus_tag="K0W59_001941"
     CDS             29747..29944
                     /locus_tag="K0W59_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HBI3022985.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            30048..31346
                     /gene="purA"
                     /locus_tag="K0W59_001942"
     CDS             30048..31346
                     /gene="purA"
                     /locus_tag="K0W59_001942"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBI3022986.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            31551..31976
                     /gene="nsrR"
                     /locus_tag="K0W59_001943"
     CDS             31551..31976
                     /gene="nsrR"
                     /locus_tag="K0W59_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HBI3022987.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLLE"
     gene            32015..34456
                     /gene="rnr"
                     /locus_tag="K0W59_001944"
     CDS             32015..34456
                     /gene="rnr"
                     /locus_tag="K0W59_001944"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBI3022988.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            34636..35367
                     /gene="rlmB"
                     /locus_tag="K0W59_001945"
     CDS             34636..35367
                     /gene="rlmB"
                     /locus_tag="K0W59_001945"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBI3022989.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
BASE COUNT         8308 a         9002 c         9693 g         8404 t
ORIGIN      
        1 cgacataatc tgaatctgag acaacatctg gcaaggctgg gacggaagtc actgtcgttc
       61 tcaaaatcgg tggagctgca tgacaaggtc atcgggcatt atctgaacat aaaacactat
      121 cagtaagttg gagtcattac cgaaaagaaa ggttaattat tggtgttagc tatataaaaa
      181 gtaacaacag caatgcatat gaataattaa aggttatgca acgtgcaaag attaaatttc
      241 tcagtttaat gacaggttgc tgtacttgcg tttgttgctc ttgccggatg cggcgtaaac
      301 gccttatccg gcctacagtt cattgcaaat ccattcaatt tcaatgcacg atgtaggcct
      361 gataagcgta gcgcatcagg caattttaat gtttaacttc cctgtttaat cagttactgc
      421 tggcgtagct ggagatccag cggcgttttg ctgggttcgc cgccaatttc tcgcgccagt
      481 ttcggcacca gatatcccga caccaatgtc agcaactcac gcataatctg ccgtgcttcg
      541 tcatcactca ccataaaatg cgccgcgccc tgtactttat ctagcacatg caggtaatac
      601 ggcatcacgc cggcatcgaa caacgcatta ctcaggtttg ccaacgtttg tgcgttatcg
      661 ttcacaccac gtaacagaac gctctggtta agcagggtga caccgacacg gcgcaattta
      721 gccatcgcct gacggaacgt ttcatctacc tcattggcat gattgatgtg attcaccagc
      781 aagatttgca gcgtagaacg ggcaaagcgt tcaaccagcg cctcggtgat acgtgccggg
      841 atcacaatcg gcagacggct gtgaatccgc agacgtttga tatgcgggat ggcttccagt
      901 tgtgtgagca accagtccag ctcgtgatct ttcgccatca gcggatcgcc gccggagaaa
      961 atcatctcgt ccagttccgg atgcgcagca acatactcaa gtgcagtctg ccagttacgc
     1021 ttgttgccct gattttcggc ataggggaag tggcgacgga agcaatagcg gcaatttacc
     1081 gcgcagccgc ctttgaccag caaaagcgcc cggttgtggt atttatgcaa caaaccaggc
     1141 actacgctgt gctgttcttc cagtgggtcg gtggagaatc cggacgcgac gacaaactca
     1201 tcttgcgagg taagtacctg acgcaaaaga ggatcgtccg gattgccttt ctccatgcga
     1261 tcgataaatg agcggggcac acgcagggca aacagctttt tggcgctgcg tccggccaac
     1321 agtttttcgt ccgcgtctat attcaaaaga cgcagaagtt catcaggatc ggtcacaaca
     1381 tcggcaagtt gcgttaacca atcttctctg gatggggtat ttagggttac aatatgcgcc
     1441 attttgtggc ttagctacca attaacaaat ttcagagggc cttatggcaa cgtactatag
     1501 caacgatttt cgtgctggtc ttaaaatcat gttagacggc gaaccttacg cggttgaagc
     1561 gagtgaattc gtaaaaccgg gtaaaggcca ggcatttgct cgcgttaaac tgcgtcgtct
     1621 gctgaccggt actcgcgtag aaaaaacctt caaatctact gattccgctg aaggcgctga
     1681 tgttgtcgat atgaacctga cttacctgta caacgacggt gagttctggc acttcatgaa
     1741 caacgaaact ttcgagcagc tgtctgctga tgcaaaagca attggcgaca acgctaaatg
     1801 gctgctggat caggcagagt gtatcgtaac tctgtggaat ggtcagccga tctccgttac
     1861 tccgccgaac ttcgttgaac tggaaatcgt tgataccgat ccgggcctga aaggtgatac
     1921 cgcaggtact ggcggcaaac cggctaccct gtctactggc gctgtggtta aagttccgct
     1981 gttcgtacaa atcggcgaag tcatcaaagt ggatacccgc tctggtgaat acgtctctcg
     2041 cgtgaagtaa tgcggttgtg gtgcggcctg caggctgcac catcacttat tcaggtcaga
     2101 gatgatgaaa cgccttatcg ttcttgtttt gcttgccagc acgctgctca cgggctgtaa
     2161 caccgcttgc ggtttcggcg aagacatcaa acatctcggc aactccatct ctcgcgctgc
     2221 cagctaattt ttcttctctt ccgaaaaatc atcagatttc catcattttt ggcgatgttg
     2281 tctattatta atttgctata ggcaaacata aataacatta cctaaaagga agacgttatg
     2341 gtgaagaaga caattgcagc gatcttttct gttctggtgc tttcaacagt attaactgcc
     2401 tgcaacacca cgcgtggcgt tggtgaagac atttctgatg gcggtaacgc gatttctggc
     2461 gcagcaacga aagcgcagca ataagcaata acggtacgac agcagtgtcg tgccgtttgt
     2521 tttttctgcg atcgtcataa aggtaatagt tgaaattccc ctgccacctg gcaaaatatc
     2581 cgttcaacca tcagctttgc aggacgacct gcaaacgcct cttttcaccg gggacggccc
     2641 caaatttccg gagcctgata tgtcctggat tatcttagtt attgctggtc tgccggaagt
     2701 ggtatgggcc gttggcctga aatataccca cggctttagc cgtttgatgc cgtgtggacg
     2761 ggtattggcg cagtcggcgc ggccatcacc ggcattgtgc tgctcggtga gtccgctaac
     2821 ccgatgcgtc tggcgagtct ggcgttaatc gtattgggga ttattggtct gaaactcagc
     2881 actcactaac taccgggctg ctgtacccaa ataaacttac tgacatcaaa cccttcccgg
     2941 gtcgcgactg ccagcatctc ctgtttcact tcgtcagaaa tggttggcgt gcgggagagt
     3001 atccacaggt agtcgcggtc cgggccgcaa accagcgcat ggcggtattc ccgatcgagt
     3061 gcaataacgt tataaccgcc atagaaagga ccaaagaatg acactttcag cgcagcgcga
     3121 gttggtgcgc cggtaaagta cgctttccct tcactctgct gccacattcc tctgtcaggg
     3181 ttatagcctt tattaatgac attcaggccg ccgtcatcac gcaggctgta tgttgcggtg
     3241 actttttcca gtccacgttc aaaacggtga tcaaaacggg caatctcata ccaggtaccg
     3301 agataacgtt tggcgtcgaa attattcact acggtcacgc cacgcggcgg cgtaggagaa
     3361 ctacaggcaa cgaccagaaa tgcagctgtc gctgcggcaa cgagagggag caggcgcata
     3421 aatgtttcct tactggtttt tttctaagtg tagatgacag caaggaaaag cgcagaaaag
     3481 gtccgaaaat tcggacccga tgaaatttta ctgtagagcg ttgagaatct gacaggcggc
     3541 ggcgactctc tctggattgg gatagttttt gttagccagc atcacgatac ccagctcttt
     3601 ttctggaata aacgcgacat agctaccaaa tccgccggtc gcccccgttt tatgtaccca
     3661 tgatgcgcgt actgcaggag ttgggggctt aatcgctttt accgggcgtg ctgccagtgc
     3721 aattttattg tcactgccgt taatgatgct gtcaggattg accggccagt ccagcatttc
     3781 ccagcccagg ccctgataca tatcgccggt ttgccagtag cgagattgtg ccagttgtat
     3841 cccttgttga agcgttttct cattgatatc aagggggggt aaattgcttt gcacccagcg
     3901 ggccatatct tcaatggtcg acttcacacc ataagcttca gcatctaacg cccctggcga
     3961 aacatgcact gccttacctt cgcgatatcc ccaggcgtaa ttcttttctt ctgggggagg
     4021 tacattaatc cacgtatggt tgagtttgag tggctggaag acacgagttt gcatcgcctg
     4081 ctcaaaactc aaaccagacg gcttcacagc cagtgcgccg aacaaaccga tactggagtt
     4141 ggcatacaga cgttgtgttc ctggagccca tgcaggctgc cagttttgat agaagcgcag
     4201 caagtcgctt gaggatttca cttcatccgg cacctgcaat ggcaggccgc cagcggtgta
     4261 ggtcgcaaga tgtaatagtg tgatcccatt ccactgttta gcggtaagtt caggccagta
     4321 ttttgttgcg ggatcgctta acttgatttc ccctcgagca atagcgtcgc caccaagcac
     4381 gcccgtaaat gttttgctga ccgaacctaa ctcaaacaac gtttgctgtg tgacgggctg
     4441 ctttttggcg atgtccgcat agccccaggt aaagtaataa ggtttaccct gataaattac
     4501 cgccaccgcc ataccgggga tcttttgttg ctctataagc ggggtaattg tgcgatgcac
     4561 aatatcgttg atttgttgag gggcagcaaa tgtggagcaa gaggcggtaa ttaataaggc
     4621 gcagagcgtc gttttgaaca taaggtctgg tttccataca aaacggcccg ccataggcgg
     4681 gccggattta cattggcgat gcgttagatt gtaacgacac caatcagcgt gacaactgtc
     4741 aggatagcag ccagaccgta gaaaacccat ttgcccgcag gcacgtggat tttcagatcg
     4801 tgcatcgcgt ggtgcatacg gtgtaaacca caccacagcg gcagaacgat catcaggaac
     4861 aggaatacgc gaccaatgaa gctctgcgcg aacgccagaa cgcgctcgta gctcagcgca
     4921 tcacccggaa acagccccag tggcagcaga atacccacca gcaggatcat caccggcgca
     4981 ataattgcgc tccacatacc accggccccg aagaggcccc agaataccgg ttcgtcagaa
     5041 cgctttggat ttggattaat catctcaggc tccttaccag tacagggcaa caaacaggat
     5101 tacgatggtg gcaaccacag ttaccgccca gagacttttg ataattggct ctggtcccat
     5161 tttttcgtct tttacaatga tattggccgc tttcggtgcc agttcaaacc aggttttggt
     5221 gtgcagcaga gctgccgtca gagtgatcag gttaatgatc acgataaccg ggttttgtaa
     5281 aaagtcgacg aatcccgccc aggcttccgg gccatttttc agggcaaaca gcccgaaaat
     5341 cagttcaatg ctgaaccaca cagccggaac cgccgtgcct tcgcgcagca tgtaaaagcg
     5401 ataaaacggc aattttttcc accaggtgga cgtcattggc cgtacatacg gtttacgttt
     5461 agtcgtcatg ttgcactcct tagcgtggtt tcagggtcgc gataagaaag tctttcgaac
     5521 tttctacttt gccctgctga atggccgcag ccggatcgac gtgtttcggg cagacttcgg
     5581 agcaatagcc cacgaaagta cagctccata caccgttctg gctgttcaac tgcgccatac
     5641 gctccttctt accgtggtcg cggctatctt cgttataacg atgcgccagc gtaatggcag
     5701 ccggaccgat gaactctggg ttcaggccaa actgcgggca cgcggcgtag cacagaccac
     5761 agttgatgca accggagaac tggtgatact ttgccatctg cgccggggtc tggatgttag
     5821 taccctgatc cgcggtgcgg gagttgccga tgatgtacgg tttgatcgct tccagacttt
     5881 cgatgaagtg ggtcatatcg accaccagat cgcgttcaat cgggaagtta gctaacgctt
     5941 caaccttcat actgtcggtg taatcacgca ggaaggtttt acatgccagt tttggcacgt
     6001 tgttaaccat catgccgcag gaaccacaaa tcgccatacg gcaggaccag cggtaactca
     6061 ggtccggtgc caggttgtct ttgatgtagc ccagcgcatc cagtaatgag gtagttgcgt
     6121 cataaggcac ttcatagaat gcgctatgcg gtgcggtatc gacttccggg ttatagcgca
     6181 ccacctcaat tttcaggttt ttcatctcag ccattcgcct tctccttatt attggctgct
     6241 tccgccttat cggctgcatc cgcttcgcca ccgtaaacgc gtttagctgg cggcagcgta
     6301 gtaatcttca cgtcgctgta ctccaggcga gtcgtgccat cagcatcgcg gaaggcgagg
     6361 gtgtgtttga gaaagttgac gtcgtcacgc tcggtgcaac cttcgtccag acgctggtgt
     6421 gcgccgcggg actctttacg tgccattgcg gagtgcgcca tacattcagc aacgttcaga
     6481 ccgtggctca gttcaatggt gtagagcagg tcggtgttga acacgctgga ggtgtcggtg
     6541 atgcgcacgc gcttgaagcg ttcctgcagc tctgccagct tgtcgatggt tttctgcatc
     6601 agttccggcg tacggtagat accgcaacct tcttccattg ccaggcccat ttcgtcgcgg
     6661 atcttcgccc agttttcgcc gccatcctgg ttaaccagat ctttcagacg ttgttcaacg
     6721 ccagctgcct gcgcttcaat tgccgcttcg ttgccattac cggcagttgc tgcacgctct
     6781 gtcgcttgtt caccggccag acggccgaag accaccagtt ccgccaggga gttggagccc
     6841 agacggtttg caccgtgcag accaacagag gaacattcac ccacggcgaa cagaccttta
     6901 atgcgggttt cacagttctg atcggtttcg ataccgccca tggtgtagtg tgcagtcgga
     6961 cgtaccggaa tcggttcttt aaccggatcg acgccaacgt acgctttcgc cagttcgcag
     7021 atgaacggca gacgttcatg cagttttttc tcgccgaggt gacgcaggtc gagataaacc
     7081 acatcaccac gcggcgtgga gatagtgttg cctttacgcc attcgtgcca gaaggcctga
     7141 gaaactttgt cgcgtggacc cagttccata tatttgtttt tcggctcgcc cagcggagtt
     7201 tccgggccca tgccgtaatc ttgcagataa cggtagccat ttttgttgac cagaataccg
     7261 ccttcaccac ggcagccttc ggtcatcagg ataccggaac ctggcagacc ggttgggtga
     7321 tactgaacga attccatgtc acgcagcgga acgccgtggc ttagcgccat acccataccg
     7381 tcaccggtaa cgatgccgcc gttggtgttg taacgataaa cgcgacccgc accgccggta
     7441 gccataacga ccgcgttagc acggatctgc accaacgtgc cttccatcat gttcattgct
     7501 accaggccgc gaacatgacc atcatcaacc agaatatcca gcacgaaatg ttcgtcaaaa
     7561 cgctggatct gcgggaattg cagagaggtc tggaacagcg tgtgcagcat atggaagccg
     7621 gtcttatcgg cggcgaacca ggtgcgctcg attttcatgc cgccgaagcg acgtacgttg
     7681 acgctaccat ccgggcgacg gctccatggg catccccaca gttccagttg ggtcatttcg
     7741 gttgggcagt ggtggacgaa ataatccacg acatcctgct cacacaacca gtcgccaccc
     7801 gctactgtat cgtgaaagtg atattcgaag ctgtcatgat cctgcgcgac agcggcggag
     7861 cccccttctg cagcaacggt atggctacgc atcgggtata cttttgagat tagtgcgatt
     7921 tttgcatttg gatttgcctg cgcggcagca attgcagcac gtaatcccgc gccaccggcg
     7981 cctacaatgg caagatcggc ttgaaaggtt tgcacgacat tcctccagat tgtttttatc
     8041 ccacagcccc gtacttcagg gtaagtgcct gaaagttacg gtctgcgaac gctattccac
     8101 tgctccttta taggtacaac agtatagtct gatggataag tctgaaattt gacgagatcg
     8161 atttttttag tgcgcaagag ggctaaatta tcactgaaga tgattaattt aattactaaa
     8221 ccatcagatc gtgctttttt tagtcactgc ccaccgctgt ttgattcctg cgtaaaattt
     8281 gtctcgccgt cgcgttgcga gtagacttcg tgcccttgtc aaaaactgga gatttaacta
     8341 tgagcgaaac ggcatcctgg cagccgagcg catccattcc taacttatta aaacgcgcgg
     8401 cgattatggc ggagatccgt cgtttctttg ccgatcgtgg agtgctggag gtggagacgc
     8461 cttgtatgag ccaggcgacg gtaaccgata ttcatttggt cccgtttgag acacgtttcg
     8521 ttggccccgg gcattcgcag gggatgaatc tctggttaat gaccagcccg gaataccata
     8581 tgaaacgcct gctggttgct ggttgtgggc cggtattcca gctgtgccgc agcttccgta
     8641 atgaagagat ggggcgttat cacaaccctg agttcactat gctggagtgg tatcgaccgc
     8701 actatgatat gtaccggttg atgaacgagg tggacgatct cttacaacag gtgctggact
     8761 gcccggcagc agaaagcctt tcttatcaac aagctttctt gcgttatctg gaaattgacc
     8821 cgctctctgc cgacaaaacg caactgcggg aagtcgcagc gaaactggat ttgagcaatg
     8881 ttgctgatac cgaagaagac cgcgacacgt tgctacaatt gctgtttacc tttggcgtag
     8941 aggcaaatat tggtaaagaa aaaccgacct ttgtgtacca ctttccagcc agccaggcat
     9001 cactggcgca aatcagtacc gaagatcatc gggtcgctga acgctttgag gtttattata
     9061 aaggtattga gctggcgaat ggtttccatg aattaacgga tgcccgtgag cagcaacaac
     9121 gctttgaaca agataaccgt aagcgcgcgg cgcgcggttt gccgcagcac cccattgacc
     9181 agaatctgat tgaagccttg aaagtcggta tgcctgactg ctccggcgtg gcattaggtg
     9241 ttgatcgtct ggtgatgttg gcgctgggcg cggagacact ggctgaagtc atcgccttta
     9301 gcgttgaccg ggcataattc tgaaaaatta ctgaattaac agcgaagaat ggcgtgataa
     9361 gcgccattct gtagcatatg ttttcatttt tccgttaaag gccacaattt aagactggat
     9421 atttgatatc atcaaggtat caattctgta ttggttttta cgctgcctgc tcaatgttgc
     9481 gcagtgcagt tcagtgggca cgtgttatac acgcgctgaa atgaaggatg gtttcatgcc
     9541 tcacacgata aaaaagatga gtctgatagg actcatattg atgatcttta cttccgtatt
     9601 tggatttgcc aatagcccat cggcttatta cttaatgggt tatagtgcga ttccctttta
     9661 tatattttct gcattgttat tctttattcc attcgcctta atgatggctg aaatgggagc
     9721 tgcttatcgc aaagaagaag gtggtatcta ttcctggatg aataatagtg tcggaccacg
     9781 ttttgccttc attggtacgt ttatgtggtt ttcctcttat atcatctgga tggtgagtac
     9841 ctccgcgaaa gtttgggtac cgttctcaac attcctctat ggtagtgaca tgacccagca
     9901 ctggcgtatt gccggactgg agcctacgca ggtggttggt ctgctggcag tagcatggat
     9961 gattctggtc accgtcgttg cttcaaaggg gattaataaa attgcccgca ttactgcggt
    10021 gggcggtatt gcagtaatgt gtctgaattt agtattgctg ttagtaagca ttactatttt
    10081 gttattaaat ggtgggcatt tcgcgcagga tattaatttc cttgcatcac cgaacccggg
    10141 ttatcagtcc ggtctggcaa tgctatcgtt tgtggtattt gctatttttg cctatggcgg
    10201 aattgaagcg gttggtggtc tggtcgataa aacggaaaat ccagaaaaga actttgccaa
    10261 aggtattgtt tttgccgcta ttgttatttc aatcggttat tcgctggcaa tatttttatg
    10321 gggcgtcagc acaaactggc agcaggtatt aagtaatggt tccgttaacc ttggcaatat
    10381 tacctatgtg ctgatgaaga gcctcggggt gacgctgggt aacgcactgc atttgtcacc
    10441 tgaagcgtca ttgtcgctgg gcgtatggtt tgcgcgtatt accggacttt cgatgttcct
    10501 cgcttatacc ggtgcgttct ttacgctttg ctattcaccg ctgaaagcca tcatccaggg
    10561 gacgccgaaa gcgttgtggc cggaaccgat gacgcgcctg aatgcgatgg ggatgccgtc
    10621 tatcgccatg tggatgcagt gcgggttggt tactgtcttc attctgctgg tttcgtttgg
    10681 tggcggtacc gcatcggcgt tctttaacaa gctgacgctg atggcgaacg tgtctatgac
    10741 gcttccttac ctgttcctcg cgctggcttt cccgtttttt aaagcacgtc aggatctcga
    10801 cagaccattt gtgattttca aaacgcgtat gtcggcaatg attgcgacgg tggttgtcgt
    10861 tctggtggtg acatttgcga acgtcttcac cattattcag cctgtggttg aagccggaga
    10921 ctgggacagc acattgtgga tgattggcgg ccctgtcttc ttctcgctgt tagcgatggc
    10981 gatttaccag aactattgca gtcgcatggc gaataaacct gagttagctc tcgactgatc
    11041 atatgaaatc ctgcaactca tcgtctctcc ggcgatgagt tgtcaggaag taatgcgtat
    11101 ggatgatact ctccagggcc ggtgataact gaagatgatg ttaagctgct aaatgagcca
    11161 cgttgacatc atttggcagc ttcatgcatt acaaacttcc cgcctgacga cctttgcccg
    11221 cagacgtcag cgttctcccc gtttgtttac cgttgaggct ttccagacgc atctggaagg
    11281 gcgggaatgg catatcgata ccgtgggcgt ggaagcctgc cagaatcagc tggtggatct
    11341 catggcgtag cggcatacgg tgacccatct cagcggcgta aatacgcagc tcgaaaatct
    11401 gaatcccctg ttgcagatcc accaggaaga cttccggtgc cgggttgtcg atcaccagcg
    11461 agcagcgacg cgctgcggtg agcaggattt ccgtcacttc ttcactgttg gcatcggcag
    11521 gggccggaat cgtcaacact acgcgcgtga ccgagtcaga gagcgaccag ttgataaact
    11581 gcgcggtaat aaacgccttg ttcggcacga ttatctcttt acggtcccag tcgctgatgg
    11641 tggtggcgcg ggtgttaatt tttgtcacgc taccagtaag atcgcggatc gttaccgtat
    11701 cgccaatgcg aatcggtttt tcgaacagga tgatcaggcc agagataaag ttggcgaaaa
    11761 tttcctgcaa accaaaaccg agaccaacac cgagcgcggc aaccagccac tgcaatttcg
    11821 accactcaat accaatcatt gagaagccga ccagcccgcc aatcagcatc agcagatatt
    11881 ttgtgatggt ggtgatggcg taacccgtac ccggcgttaa atccaggtgc tgcaaaatcg
    11941 ccagttccag cagcgcgggc aggttgcgca ccagctgcgt ggtgatgata aataccagaa
    12001 tggcaatcag caccgcaccg agggtaattg gctccagact ttctacgccc tgtaccgtgg
    12061 aggtgacatc ccacagcgaa atattttcga ggaagccgaa agcggaatgg atttctgacc
    12121 acagcacaat gaccgaacgc agggcgatca acatcaaaat tgagcgcacc agccgcaagg
    12181 attgcgcact gatggcatcg agatcgactt cgctttcatc gacttcaatt gctccttccg
    12241 ggctactgtg atgatgcgct tcctcttcgc cacgcgcacg ttgcgctaac atctctgccc
    12301 ggcgatgctt cgcccggtca aacgccagcc tgcgacgctg gatcagcatc cagcggcgga
    12361 taacgtgata aaccaccagt agcaggaacc agatggcaac cgaggtttca agcctcgcta
    12421 acagtgcctg cgccgttgcc agataaccca ccgccgacgc cagaatggca accagtggtg
    12481 cgccaatcat catgttccac agcatatggt tggtaatgtt gtcgccgctg ccttctttgt
    12541 tgagatacaa cggaataccg gcttttttca ggcttagggt gaccaccgcc agcgcaccgc
    12601 aaatgaggat aaagcaaagc cgtcccagcg aaccggaaaa ttcgcggtcg tcgaggttat
    12661 cgaacatcat cagcgccata atcagcccga tgctcatcag gtagtagcgc atcccacggg
    12721 aaacacgttc gcgcggccag ccaaaatgag cgataaacaa gccgttcggg cgggcaaagg
    12781 tggcgcaaat catcactacc cacagcagcg gcacggtggc cgttacacca tcgccaatcg
    12841 cgaccgccag cggataaggc cacgcctcgc gcaagccgta acccagcgtc atccacagca
    12901 ccggtaacgg tgacgcgacg agaatcgacc agaaaagagt gcgcaacgtc agccagaagt
    12961 gatcctgagt cactttgccg actttcgccg ccgaacgttc aagaaaacgg gtgaaatagc
    13021 ggcgcgagta aatactgcaa ccgaccagaa tcaacgcgcc aaacagcggc aaaattgtct
    13081 ctttgctggt cagcatcatc acactggctt tgcccaactg actgaaggtg tccagcgaaa
    13141 tgagacgacg cagatcctgg gcgatttcca gcggccaggc gatggtcatc gggcgcacgt
    13201 cagaggtcca gaacagatag cggtgcgttg cttcgttcac ctctttcagc gcatcctcca
    13261 gttgcccgtt ggagactttc agcttggtca gttccaatag tagcgtgtcg ccaccctgca
    13321 acaatgagtt cagcaactca cgctgagtgc gcagttgtgc ttccagaata cggttttgct
    13381 cggcggtcag cggctgaccg tcggcctgat ggatttgccg tagcagcggc tgtttattaa
    13441 gtaaatcctc ataacgtaac cgttgcacac gcaactgcgc catttcggta tcaagctgtt
    13501 gtggttttgg catttctggt agccgtgcca cctgcgcccg cagcgcttcg ccgagcaggt
    13561 tggacgatcc cagccattgc gactgttcac gcagcgtatt caacgcctgc cggacctgta
    13621 acgtctggct ggcagcctga cgctgttgcg aggcaacgag ctccatccgc tgcgcctgtt
    13681 gattcagcgc tgccgatagt tcgcggttaa ttttgaattg cgcgacgata tctttcggca
    13741 aatcggcact gttttctgcc agtaattcgg tactttccag cgcccgctcc gcctcaagct
    13801 gacgttggct attcaactga ttacgtaagc cctgcaaata cgcatccagt tgctggctct
    13861 ctttttccgc cagctctgag cgtaagcgcg ctaattcctg gcggttattg gcagacagct
    13921 gcgccagctc cagttcatca acgagcgcct taagacgtgc agagtcagac tgcaacgcga
    13981 aattttgtgc ctgattgagc ggagtattgc cggtaagcgt tcccaggcgg cgctcgatct
    14041 catttaactg acggcgggcg tcggtttgct gttgcggcag ttgattcagc gaatcggcaa
    14101 tctcgcgggc gcgctcctgc tcttgctggg cctgacggct tttatccagc aactggctgc
    14161 tgacctggag aatttcctga ttcagcgcgt cggtagacat tcccggcgac acgctgcgcg
    14221 gctcgtcacg catgttgttt aattgtgcgc gcagagtagc ggagagtttc ggataattat
    14281 cgataacttg ctgatattgt ttgatgcgct caagggaacc ttttcgttcc tcaagcgcat
    14341 ttaaggcaga ctggagcgcc tctacgactt ccggctgtgc gggtttcgcc gcttttgcct
    14401 gctccagttc ctgagtgatt tgtttgctat cgggggccgt cgcggcgtac gccccccaac
    14461 tgaggcacca ggccatcaga aaagtgataa tcaggcgcac gtcagcgttt cctttgatgg
    14521 attagacctg gtctttttta tcgtcaacca atggcctggc gtcgtgttct gcttcgatct
    14581 cttcagcagg aagtggggcg ggttcagcgt ctggcgtaac aaaggtttcg gtagatactg
    14641 ccagcggctg gccaattttc gtgacagaca ggctttccag ttgctcaacc agattcactt
    14701 tacccggtgc aaacaggttg ataacggtgg aaccgagttt aaagcgaccc atttcctggc
    14761 ctttcagcag tgccacagaa ccgtcgtttt ccccggcagg ccaggtccag cgcttgatga
    14821 taccttcgcg cggcggcgta atggtgcccg cccagaccgt ctcaatgctg ccaacaatcg
    14881 tcgctccgac cagaatctgc gccattgggc caaattcggt atcgaaaagg caaatcacgc
    14941 gttcgttacg ggcaaacaga ttcggcacgt tctgagcggt gagatggtta acggaaaaga
    15001 gatcgcccgg cacgtagatc atctcacgca gaataccgtt gcacggcatg tgtacgcgat
    15061 ggtagtcacg cggggagagg taagtggtca caaacgtacc gttgcggaac aggtccgcca
    15121 tcagatagtt gcctgccagc agggcttcga ggctgtagtt gtggcctttg gcttgcagga
    15181 ttttatcttc ttcgatttta cccaactggc tgataacgcc atcggcaggc atgaccagta
    15241 cattcggatc ggtatcgatt gggcgtactt cgtcacgcag cggacggaca aagaattcgt
    15301 taaaggtgcg gtagctggcg gtgtccggct tttgcgcctc tttcatgtcg accttgtagt
    15361 atttaacgaa cagatcgata accagttttg tcagccatcc tgcccgcttg cttgcgcccc
    15421 aacccgccag gcgagtaagc catagtttcg gcagaatgta ctgtagcgaa agtttaaatg
    15481 aatttaacaa ggtagcctcc aggccattgt tttgtcgttc ctgatccggc caacatgccg
    15541 gatcctgaaa aaaaggggac gattctaacg acggttagct taattgtcag tcatccgtat
    15601 cagaaaagtt tttacgcgtt tttacctgcg ccatgctttc cagaatacgg tgatagtttt
    15661 cgaaacgggt ttccgcgatt ttcccttcct caaccgcttc ccggatagcg cagcccggat
    15721 cggtatcgtg tttgcaatcg cgatatttac acagacctaa atagtcatgg aattcgacaa
    15781 agccctgagt gatttgttcc ggctccaggt gccagaggcc gaactcacgc acaccagggg
    15841 aatcaatcac atcaccgccg tgcgggaagt gatacagccg agcggcggtc gtggtgtgct
    15901 gaccgagccc cgagttgtca gagacatcgt tggtcaggat ctctttttgc agccccagca
    15961 acgcgttcag caggctggat ttaccgacgc cagactgccc ggcaaaaatg ctgatgcgcc
    16021 cggtcaacgc ctcttccagc ggtttcagcc catcctgagt atggctggaa accatcaata
    16081 cgcgataacc gatgttgcgg tagatatcca tctgctcgtt gacgaacgcc atgccttcgt
    16141 cgtccagcag gtctatcttg ttgagcacga taatcggctc aatctgcaag gtttcgcagg
    16201 ccaccaggta acggtcgata atattgagcg acagctccgg caaaatggcg gagacaatga
    16261 caatctggtc gatgttggcg gctatgggtt tcacgccgtc gtagaaatcc ggacgcgtta
    16321 acaccgaggt gcgctcatgc accgcttcca cgatcccttt gacatttacg ccttccgccg
    16381 ccggtttacc cggacgccag actacgcggt cgccggttac cagcgaacgg atggtacggc
    16441 gaatattgca gcggtgaacg tcgccatcgg cggattccac atcagcgtgc ataccaaagc
    16501 ggctgatgac gataccttca tcaggctcgc caaacagatt gtcgtcgtag tcgggcttct
    16561 ccttagacgt tttaagacga cgctggtgat tggcgttcac gcggcgctgc tggcctttgg
    16621 agagtttatt tttactcaat cgtactgact cctggtcgcc cctgatgggc aaaacatcta
    16681 tgatacacgc aattgtggat caatatagtc actgtgaatg ggtggaaaat agcatgagtg
    16741 ccaatgaaaa caacctgatt tggatcgatc ttgagatgac cggtctggat cccgagcgcg
    16801 atcgcattat tgagattgcc acgctggtga ccgatgccaa tctgaatatt ctggcagaag
    16861 ggccgaccat tgcagtacat cagtccgatg aacagctggc gctgatggat gactggaacg
    16921 tgcgcaccca taccgccagc gggctggtag agcgcgtgaa agcgagcacg atgggcgatc
    16981 gggaagctga actggcaacg ctcgaattct taaaacagtg ggtgcctgcg ggaaaatcgc
    17041 cgatttgcgg taacagcatc ggacaggacc gtcgtttcct gtttaaatac atgccggagc
    17101 tggaagtcta cttccactac cgttatctcg atgtcagcac cctgaaagag ctggcgcgcc
    17161 gctggaagcc ggaaattctg gatggtttta ccaagcaggg gacgcatcag gcgatggatg
    17221 atatccgtga atcggtggcg gagctggctt actaccgcga gcattttatc aagctgtaaa
    17281 atttttcctg gtcacgtaag cgccggaatc cacttgcggc ggcgtgattt tgacgctaaa
    17341 tcgaacgtct tgctgataat ttcagcactt gagataaaaa cgcaaaaaaa acttttttgg
    17401 ggggttgcag agggaaagat ttctcgtata atgcgcctcc cgtaacgacg cagaaatgcg
    17461 aaaattacga aagcaaaatt aagtagtacg cgggaatagc tcagttggta gagcacgacc
    17521 ttgccaaggt cggggtcgcg agttcgagtc tcgtttcccg ctccaaaatt tgaaaagtgc
    17581 tgcaaagcac agaccaccca atcgcagtat ttcgaattgc gacaaggcgg caactgaacg
    17641 aatccttggg aacttacaaa agtatgtgac taaggtgagt gaaggcagcc aaggcagtag
    17701 cggttcgaaa gacgaagatt aagcgggaat agctcagttg gtagagcacg accttgccaa
    17761 ggtcggggtc gcgagttcga gtctcgtttc ccgctccaaa atttgaaagt gctgtaaggc
    17821 acagaccacc caagcgggaa tagctcagtt ggtagagcac gaccttgcca aggtcggggt
    17881 cgcgagttcg agtctcgttt cccgctccaa aatttgaaag tgctgtaagg cacagaccac
    17941 ccaagcggga atagctcagt tggtagagca cgaccttgcc aaggtcgggg tcgcgagttc
    18001 gagtctcgtt tcccgctcca aattcttctc tcaataaaat atccacagcg acgcgatgcg
    18061 ttattgctgg tttttgttgt ctctgacaaa ctcttgtaaa cagagttatc cacagcctca
    18121 ggctgtaatc ttaatttcaa agaaacttcg cacggtgaat ggtatttttt aacctattga
    18181 taaataagtt aaaaatcaag attccgtttt gtcgagtcga tcacttgacg attttattcg
    18241 tcttgaattg caatgcgttt ttatttttat tcacaagctg tggatgaatc aggcgtcacg
    18301 cggtaaccct ttttcaatca cccgaaccag acgctgtttt ttcggcaatt gcacttcgac
    18361 tatgcacgca tttcgtctct ctatttgctg cgcaatcgcc cacgctatgt gctcatcgag
    18421 aagtgggtgc tcacctttac gactttccag cgctgtcaaa atcgtttcat cccaaggggc
    18481 attgcctaat gccacggcga tattacgcag ccagcgcagg tgacctatac ggcgaatcgc
    18541 cgatccttcc gtgactttta aaaacttctc ttcgctccag gcaaataact caatgagttc
    18601 cggtgcgtgt agcggcttac gcgggctgaa atcgtcttct gtagtgagtt gcgaatagcg
    18661 attccacggg cagataagct ggcagtcatc gcaaccgtaa atacggtttc ccattaacgg
    18721 tcgcaactct tccgggatcg ccccttcaag ttcgatggtg agataagaga tacagcggcg
    18781 agcatcgacg gtatatggct cgacgatggc accggtcggg caaatcgtca tacaggccac
    18841 gcatttgccg catccttcct cgactggttg atccacgggc agcggaatat cgaccagcaa
    18901 ttcgcctaaa aagaagaacg aaccggcctc gcgattgagg ataagtgagt gcttacctgt
    18961 ccagccgagc ccagcttttt cagctaacgg gcgctcgaga ataggcgcag aatcgacaaa
    19021 cggtctaaaa ttcagcgaaa cacaatgttg ctgaatcatc tcgcccagct ttttgagtcg
    19081 gttgcgcaga agtttgtgat agtcacggcc cagcgcataa cggctaacat agccgagttt
    19141 ggggtttttc agcgtgctgg caaatgcggc gttagcagga aggtaattca tccgcacgct
    19201 gattacacgc agcgtaccgg gtaataactc atgggggcga gcgcgcagca taccgtgacg
    19261 tgccatccag tccatttcgc cgtggtattg tttgtccagc catgcttgca gtttgggctc
    19321 ggactcgctg agatcggtat cggtaatacc tacctgctga aagcccagtt ccagccccca
    19381 ctgtttaatt ttttgcgcta actgattgag atcgaggggc tctgacatga cggaccatac
    19441 aatgaagaaa aaccccgtaa gtataccaca caccgtctgg tacgccgacg atatccgccg
    19501 cggagaacgc gaggcggcag atgtgctggg gctcacactc tatgagctga tgcttcgcgc
    19561 tggcgaggcc gcattccagg tgtgtcgttc ggcgtatcct gacgcccgcc actggctggt
    19621 gctgtgcggt catggtaata acggcggcga tggctacgtg gtcgcgcgac tggccaaagc
    19681 ggtcggcatt gaggtcacgt tgttggccca ggagagcgac aaaccgttgc cggaagaggc
    19741 cgcgctggca cgcgaagcat ggttaaacgc gggtggcgag atccatgctt cgaatattgt
    19801 ctggcccgaa tcggtagatc tgattgttga tgcgctgctc ggtaccggct tgcagcaagc
    19861 gccccgcgaa tccattagcc agttaatcga ccacgctaat tcccatcctg cgccgattgt
    19921 ggcggttgat atcccttccg gcctgctggc tgaaaccggc gctacgccag gcgcagtgat
    19981 caacgccgat cacaccatca cttttattgc gctgaaacta ggcttgctca ctggaaaagc
    20041 gcgggatgtt acaggacaac tgcattttga ctcactgggg ctggatagct ggctggcagg
    20101 tcaggagacg aaaattcagc ggttttcggc agaacaactt tctcactggc tgaaaccgcg
    20161 tcgcccgact tcgcataaag gcgatcacgg gcggctggtg attatcggtg gcgatcacgg
    20221 cacggcgggg gctattcgta tgacggggga agcggcgcta cgtgctggtg ctggtttagt
    20281 ccgagtactg acccgcagtg agaacattgc gccgctgctg actgcacgac cagaattgat
    20341 ggtgcatgaa ctgactatgg actctcttac cgaaagcctg gaatgggccg atgtggtggt
    20401 gattggtccc ggtctgggcc agcaagagtg ggggaaaaaa gccctgcaaa aagttgagaa
    20461 ttttcgcaaa ccgatgttgt gggatgccga tgcattgaac ctgctggcaa tcaatcccga
    20521 taagcgtcac aatcgcgtga tcacgccgca tcctagcgag gccgcacggt tgttaggctg
    20581 ttccgtcgct gaaattgaaa gtgaccgctt acattgcgcc aaacgtctgg tacaacgtta
    20641 tggcggcgta gcggtgctga aaggtgccgg aaccgtggtc gccgcccatc ctgacgcttt
    20701 aggcattatt gatgccggaa atgcaggcat ggcgagcggc ggcatgggcg atgtgctctc
    20761 tggtattatt ggcgcattgc ttgggcaaaa actgtcgccg tatgatgcag cctgtgcagg
    20821 ctgtgtcgcg cacggtgcgg cagctgacgt actggcggcg cgttttggaa cgcgcgggat
    20881 gctggcaacc gatctctttt ccacgctaca gcgtattgtt aacccggaag tgactgataa
    20941 aaaccatgat gaatcgagta attccgctcc ctgatgagca ggcaacatta gacctgggcg
    21001 agcgggtagc gaaagcctgc gatggagcaa ccgtaatcta tctgtatggc gatttaggcg
    21061 caggtaaaac cacctttagc cggggctttt tacaggctct gggtcatcag ggtaatgtca
    21121 aaagccccac ttatacgctg gtcgaaccct atacgctcga caacttaatg gtctatcact
    21181 ttgatttgta ccgccttgcc gatcccgagg agctggagtt tatggggatc cgcgattatt
    21241 ttgccaacga tgccatctgc cttgtggagt ggccacaaca aggtacaggt gttcttcctg
    21301 acccggatgt cgaaatacac attgattatc aggcacaagg ccgtgaggcg cgcgtgagtg
    21361 cggtttcctc tgcgggtgaa ttgttgctgg cgcgtttagc cggttaacct ttgaaaggtg
    21421 gcgggatgat gtatcgcatc agaaattggt tggtagcgac gctgctgctg ctgtgcacgc
    21481 cggtgggtgc cgcgacgctc tctgatattc aggtttctaa cggtaatcaa caggcacgga
    21541 taacgttgag ttttattggc gatcctgatt atgcgtttag ccatcaaagc aaacgcaccg
    21601 tggcgctcga tatcaaacaa acgggcgtga ttcagggact gccgttgttg ttcagcggca
    21661 ataatctggt gaaggcgatt cgctctggaa cgcctaaaga tgcacaaacg ctacggctgg
    21721 tggtcgatct taccgaaaac ggtaaaaccg aagcggtgaa gcggcagaat ggcagcaatt
    21781 acactgtcgt ctttacgatt aacgccgatg tgccgccacc gcctcctccg ccgcccgtgg
    21841 ttgcgaaacg cgttgaaacg cctgcggttg tcgcaccgcg cgtcagcgaa ccggcgcgca
    21901 atccgtttaa aacggaaagt aaccgcacta cgggtgttat cagcagtaat acggtaacgc
    21961 gtccggcagc gcgcgcgacg gctaacactg gcgataaaat tatcatcgct attgatgccg
    22021 gacacggcgg tcaggaccct ggcgctatcg gccccggtgg tacgcgggag aaaaatgtca
    22081 ccatcgccat cgcgcgtaaa ttgcgtactt tgctcaatga cgatccgatg tttaaaggcg
    22141 ttttaacccg tgacggggat tactttatct cggtgatggg gcgcagtgat gtggcacata
    22201 agcaaaacgc caatttcctc gtgtcgattc acgctgatgc cgcaccgaac cgcagtgcga
    22261 ctggcgcttc cgtattggtg ctctctaacc gtcgcgccaa cagtgaaatg gccagctggc
    22321 tggagcagca cgagaaacag tcggagctgc tgggtggggc gggtgatgtg ctggcgaaca
    22381 gtcagtctga cccctattta agccaggcgg tgctggattt acagttcggt cattcccagc
    22441 gggtagggta tgatgtagcg accagtatga tcagtcagtt gcaacgcatt ggcgaaatac
    22501 ataaacgtcg accagaacac gccagccttg gcgttctgcg ctcgccggat atcccatcag
    22561 tactggtcga aaccggtttt atcagcaaca acagcgaaga acgtttgctg gcgagcgacg
    22621 attaccaaca acagctggca gaagccattt acaaaggcct gcgcaattac ttccttgcgc
    22681 atccgatgca atctgcgccg cagggtgcaa cggcacaaac tgccagtacg gtgacgacgc
    22741 cagatcgcac gctgccaaac taaggacgat tgatgccaat tcaggtctta ccgccacaac
    22801 tggcgaacca gattgccgca ggtgaggtgg tcgagcgacc tgcgtcggta gtcaaagaac
    22861 tagtggaaaa cagcctcgat gcaggtgcga cgcgtatcga tattgatatc gaacgcggtg
    22921 gggcgaaact tatccgcatt cgtgataacg gctgcggtat caaaaaagat gagctggcgc
    22981 tggcgctggc tcgtcatgcc accagtaaaa tcgcctctct ggacgatctc gaagccatta
    23041 tcagcctggg ctttcgcggt gaggcgctgg cgagtatcag ttcggtttcc cgcctgacgc
    23101 tcacttcacg caccgcagaa cagcaggaag cctggcaggc ctatgccgaa ggacgcgaca
    23161 tggacgtgac ggttaaaccg gcggcgcatc cggtgggaac gacgctggag gtgctggatc
    23221 tgttctacaa caccccggcg cggcgcaaat tcctgcgcac cgagaaaacc gaatttagcc
    23281 atattgatga aatcatccgc cgcattgcgc tggcgcgttt cgacgtcacg atcaacctgt
    23341 cgcataacgg taaaattgtg cgtcagtacc gcgcagtgcc ggaaggcggg caaaaagaac
    23401 ggcgcttagg cgcgatttgc ggcaccgctt ttctcgaaca agcgctggcg attgaatggc
    23461 aacacggcga tctcaccctg cgcggctggg tggccgatcc aaatcacact acgcccgcac
    23521 tggcggaaat tcagtattgc tacgtgaatg gtcgcatgat gcgcgatcgc ctgatcaatc
    23581 atgcgatccg ccaggcctgc gaagacaaac tgggggccga tcagcaaccg gcatttgtgt
    23641 tgtatctgga gatcgacccg catcaggtgg acgtcaacgt acaccccgcc aaacacgaag
    23701 tgcgtttcca tcagtcgcgt ctggtgcatg attttatcta tcagggcgtg ctgagcgtgc
    23761 tacaacagca actggaaacg ccgctaccgc tggacgatga accacaaccc gcaccgcgtg
    23821 ccatcccgga aaaccgcgtg gcggcggggc gcaatcactt tgcagaaccg gcagctcgtg
    23881 agccggtagc tccgcgctac tctcctgcgc ccgcctcagg cagtcgtccg gctgccccct
    23941 ggccgaatgc gcagccaggc taccagaaac agcaaggtga agtgtatcgc cagcttttgc
    24001 aaacgcccgc gccgatgcaa aaaccaaaag cgccggaacc gcaggaacct gcacttgcgg
    24061 cgaacagtca gagttttggt cgggtactga ctatcgtcca ttccgactgt gcgttgctgg
    24121 agcgcgatgg caacatttca cttttatcct tgccagtggc agaacgttgg ctgcgtcagg
    24181 cacaattgac gccgggtgaa gcgcccgttt gcgcccagcc gctgctgatt ccgttgcggc
    24241 taaaagtttc tggcgaagaa aaatcggcat tagaaaaagc gcagtctgcc ctagcggaat
    24301 tgggtattga tttccagtca gatgcacagc atgtgaccat cagggccgtg cctttaccct
    24361 tacgccaaca aaatttacaa atcttgattc ctgaactgat aggctacctg gcgaagcagt
    24421 ccgtattcga acctggcaat attgcgcagt ggattgcacg aaatctgatg agcgaacatg
    24481 cgcagtggtc aatggcacag gccataaccc tgctggcgga cgtggaacgg ttatgtccgc
    24541 aacttgtgaa aacgccgccg ggtggtctgt tacaatctgt tgatttacat ccggcgataa
    24601 aagccctgaa agatgagtga tatcagtaag gcgagcctgc ctaaggcgat ttttttgatg
    24661 gggccgacgg cctccggtaa aacggcgtta gccattgagc tgcgtaaaat tttaccagta
    24721 gagttgataa gcgttgattc tgcccttatt tacaaaggga tggatatcgg gacggcgaag
    24781 ccgaacgctg aagagttact cgccgcgccg caccgattgc tggatattcg cgatccgtcg
    24841 caggcttact cggctgctga ttttcgccgc gatgcgctgg cggaaatggc cgatatcacc
    24901 gcggcggggc ggatcccact gttagtgggc ggtacgatgt tgtatttcaa ggcattgctg
    24961 gaagggttgt cgccgctacc gtcggcagac ccggaagtgc gggccagaat tgagcaacag
    25021 gcggcagagc aaggttggga gtcattgcat cgtcaacttc aggtggtaga tccggttgcg
    25081 gcagcaagga ttcatccaaa tgatccacaa aggctttccc gggcactgga agtttttttc
    25141 atttcgggta aaactttaac ggaactgacg caaacgtcag gagacgctct accgtatcag
    25201 gtgcatcagt tcgccatcgc cccggcgagc cgtgaactgc tccatcaacg cattgagcag
    25261 cgttttcatc agatgttggc ttcaggtttt gaagcagaag tccgggcgct ttttgcccga
    25321 ggagatttgc atacggactt gccttccatt cgttgcgtgg gttatcgcca gatgtggtct
    25381 taccttgaag gcgaaatctc atacgatgaa atggtttatc gaggtgtttg cgccacgaga
    25441 cagttggcga agcggcagat aacctggctg cgtggttggg aaggggttca ctggcttgac
    25501 agtgaaaaac cagaacaggc gcgtgacgaa gtattacagg ttgttggtgc tatcgcaggc
    25561 tgaatgtgta caattgagac gtatcgtgcg caattttttc agaatcgaaa ggttcaaagt
    25621 acaaataagc atataaggaa aagagagaat ggctaagggg caatctttac aagatccgtt
    25681 cctgaacgca ctgcgtcggg aacgtgttcc agtttctatt tatttggtga atggtattaa
    25741 gctgcaaggg caaatcgagt cttttgatca gttcgtgatc ctgttgaaaa acacggtcag
    25801 ccagatggtt tacaagcacg cgatttctac tgttgtccca tctcgcccgg tttctcatca
    25861 cagtaacaac gccggtggcg gtaccagcag taactaccat catggtagca gcgcacagaa
    25921 tacttccgcg caacaggaca gcgaagaaac cgaataaggt ttcgggctgt ttttttacac
    25981 ggggagccag cgatcctgcg ttccccgctg atctatttag agggttatac gcttgtttga
    26041 ccgttatgat gctggtgagc aggcggtact ggtacacatc tattttacgc aagacaaaga
    26101 tatggaagac ctccaggagt ttgaatctct ggtctcttcc gccggtgtcg aagcattgca
    26161 ggtgattacc ggtagccgta aagcgccgca cccaaagtat tttgtaggtg aaggtaaagc
    26221 agttgaaatt gcggaagctg tcaaagcgac gggtgcttcg gtcgttcttt ttgaccatgc
    26281 cctgagcccg gcgcaagagc gtaacctgga gcgtttgtgc gagtgtcgtg ttatcgaccg
    26341 caccggcctt attttagata ttttcgccca acgtgcgcgt acccatgagg gtaagttgca
    26401 ggttgagctg gcgcagctgc gccatctggc tacgcgcctg gtgcgtggct ggacccacct
    26461 tgaaagacag aaaggcggga taggtttgcg tggtccgggt gaaacccagc tcgaaaccga
    26521 ccgtcgtttg ttgcgtaatc gcatcgtgca gatacagtcg cgcctggaaa gagttgaaaa
    26581 gcagcgtgag caggggcggc aatcgcgtat caaagccgac gttcctactg tttcgctggt
    26641 gggatatacc aacgccggta aatctaccct tttcaatcgc atcaccgaag cgcgggtcta
    26701 cgcggcagac cagttgtttg ccaccctcga cccgacgttg cggcgtattg acgttgcaga
    26761 tgtcggtgaa accgtacttg cagataccgt agggtttatt cgccacctgc cgcacgatct
    26821 ggtggcagca tttaaagcca cgttacaaga gacgcggcaa gccacattac tgctgcacgt
    26881 cattgatgcg gcggatgtgc gtgtacaaga aaacatcgaa gcggtgaata cggttcttga
    26941 agagatcgac gctcacgaga tcccaaccct gctggtgatg aacaagatcg atatgctgga
    27001 agatttcgaa ccgcgtattg atcgggacga agagaacaaa ccgatccgtg tctggctttc
    27061 cgcacagacc ggagcgggga taccacagct ttttcaggct ttgacggagc ggctttccgg
    27121 cgaggtggcg cagcatacat tgcgtctgcc accgcaggaa gggcgtctga ggagtcgttt
    27181 ttatcagctt caggcaatag aaaaagagtg gatggaggag gacggcagcg taagtctgca
    27241 agttcgtatg ccgatcgttg actggcgtcg cctctgtaaa caagaaccgg cgttgatcga
    27301 ttacctgatc taacggcgta gcgtctgaag cgtggagtca tatcctctgg cgtcgaaaga
    27361 caacagggat caccgcataa caaatatgga gcacaaacat ggcgtggaat cagcccggta
    27421 ataacggaca agaccgcgac ccgtggggaa gcagcaaacc tggcggcaac tctgagggaa
    27481 atggaaacaa aggcggtcgc gatcaagggc cacctgattt agatgatatc ttccgcaaac
    27541 tgagcaaaaa gctcggtggt ctgggcggcg gtaaaggcac cggatctggc ggtggcagtt
    27601 catcgcaagg cccgcgcccg cagcttggcg gtcgtgtcgt taccatcgca gcggcagcga
    27661 ttgtcattat ctgggcggcc agtggtttct ataccattaa agaagccgaa cgcggcgtgg
    27721 taacacgctt tggtaaattc agccatctgg ttgagccggg tctgaactgg aaaccgacgt
    27781 ttatcgacga agtcaaaccg gtgaacgtgg aagccgtgcg tgaactggcc gcttctggtg
    27841 tgatgctgac gtcggacgag aacgtagtgc gcgttgagat gaacgtgcag taccgcgtca
    27901 ccaatccgga aaaatatctg tatagcgtga ccagcccgga tgacagcctg cgtcaggcta
    27961 ccgacagcgc cctgcgtgga gttatcggta aatacaccat ggaccgcatt ctgacggaag
    28021 gtcgtaccgt gattcgtagc gatactcagc gcgaactgga agagacgatt cgtccgtatg
    28081 acatgggtat cacgctgctg gacgtcaact tccaggctgc tcgtccgccg gaagaagtaa
    28141 aagcggcgtt tgacgatgcg attgccgcgc gtgaaaacga acagcaatac attcgtgaag
    28201 cagaagcgta taccaacgaa gttcagccgc gtgcgaacgg tcaggcgcaa cgtatcctcg
    28261 aagaggcgcg tgcgtacaag gcccagacca tcctggaagc tcagggtgaa gtggcgcgct
    28321 ttgctaaact tctgccggaa tataaagccg cgccggaaat tactcgcgag cgtctgtata
    28381 tcgagacgat ggaaaaagtg ttgggtaaca cccgcaaagt gctggttaac gataaaggtg
    28441 gcaacctgat ggttctgccg ttagaccaga tgttgaaagg tggtaacgcc cctgcggcga
    28501 agagcgataa cggtgccagc aatctgctgc gtctgccgcc agcctcttcc tccacaacca
    28561 gtggagcaag caacacgtcg tccaccagtc agggcgatat tatggaccaa cgccgcgcca
    28621 acgcgcagcg taacgactac cagcgtcagg gggaataacg atgcgtaagt cagttatcgc
    28681 gattatcatc atcgtgctgg tagtgcttta catgtctgtc tttgtcgtca aagaaggtga
    28741 gcgcggtatt acgctgcgtt ttggtaaggt actgcgtgac gatgacaaca aacctttggt
    28801 ttatgagcct ggtctgcatt tcaagatacc gttcattgaa acggtgaaaa tgctcgacgc
    28861 acgtattcag accatggaca accaggccga ccgctttgtg accaaagaga agaaagacct
    28921 gatcgtcgac tcttacatca aatggcgcat cagcgatttc agccgttact acctggcaac
    28981 gggtggtggc gacatttcgc aagcggaagt gctgttgaaa cgtaagttct ctgaccgtct
    29041 gcgttctgaa attggtcgcc tggacgtgaa agatatcgtc accgattccc gtggtcgtct
    29101 gaccctcgaa gtacgtgacg cgctgaactc cggttctgcg ggtacagaag atgaagttac
    29161 taccccggca gcagataacg ccattgccga agcggcagag cgcgtaacgg ctgagacgaa
    29221 gggcaaagtt ccggtcatca acccgaacag tatggcggcg ctgggtattg aagttgtcga
    29281 tgtgcgtatc aagcagatca acctgccgac cgaagtgtct gaagcgatct acaaccgtat
    29341 gcgcgccgag cgtgaagcgg tagcgcgtcg tcaccgttca caaggtcagg aagaagcgga
    29401 aaaactgcgc gcgactgccg actatgaagt gaccagaacg ctggcagaag ctgagcgtca
    29461 gggccgcatt atgcgtggtg aaggcgatgc cgaagcagcc aaactgtttg ctgatgcatt
    29521 cagtaaagat ccggacttct acgcattcat ccgtagcctg cgtgcttatg agaacagctt
    29581 ctctggcaat caggacgtga tggtcatgag cccggatagc gatttcttcc gctacatgaa
    29641 gacgccgact tccgcaacgc gttaatataa cgactgcggt acaggtcaat aaagccaccg
    29701 catcctcagg gatgtcggtg gttttctttt tctataagga taatgaatga attcgacaat
    29761 ctggctggcg cttgccctgg ttttggtact ggaaggttta gggccgatgc tttacccgaa
    29821 ggcatggaag aagatgatct ctgcgatgac caatttgccc gataatattt tacgtcgttt
    29881 tggcggtgga cttgtggttg cgggcgttgt ggtctactac atgttgagga aaacgattgg
    29941 ctgaacaaaa aacagactgt tcgaggtcat ttttgagtgc aaaaagtgct gtaactctga
    30001 aaaagcgatg gtagaatcca tttttaagca aacggtgatt ttgaaaaatg ggtaacaacg
    30061 tcgtcgtact gggcacccaa tggggtgacg aaggtaaagg taagatcgtc gatcttctga
    30121 ctgaacgggc taaatatgtt gtacgctacc agggcggtca caacgcaggc catactctcg
    30181 taatcaacgg tgaaaaaacc gttctccatc ttattccatc aggtattctc cgcgagaatg
    30241 taaccagcat catcggtaac ggtgttgtgc tgtctccggc cgcgctgatg aaagagatga
    30301 aagaactgga agaccgtggc atccccgttc gtgagcgtct gctgctgtct gaagcatgtc
    30361 cgctgatcct tgattatcac gttgcgctgg ataacgcgcg tgagaaagcg cgtggcgcga
    30421 aagcgatcgg caccaccggt cgtggtatcg ggcctgctta tgaagataaa gtggcacgtc
    30481 gcggtctgcg tgttggcgac cttttcgaca aagaaacctt cgctgaaaaa ctgaaagaag
    30541 tgatggaata tcacaacttc cagttggtta actactacaa agctgaagcg gttgattacc
    30601 agaaagttct ggatgatacg atggctgttg ccgacatcct gacttctatg gtggttgacg
    30661 tttctgacct gctcgaccag gcgcgtcagc gtggcgattt cgtcatgttc gaaggtgcgc
    30721 agggtacgct gctggatatc gaccacggta cttatccgta cgtaacttct tccaacacca
    30781 ctgctggtgg cgtggcgacc ggttccggcc tgggcccgcg ttatgttgat tacgttctgg
    30841 gtatcctcaa agcttactca actcgtgtag gtgcaggtcc tttcccggcc gaactgtttg
    30901 atgaaactgg cgagttcctc tgcaagcagg gtaacgaatt cggtgcaact acgggtcgtc
    30961 gtcgtcgtac cggctggctg gacaccgttg ccgttcgtcg tgcggtacag ctgaactccc
    31021 tgtctggctt ctgcctgact aaactggacg ttctggatgg cctgaaagag gttaaactct
    31081 gcgtggctta ccgtatgccg gatggtcgcg aagtgactac cactccgctg gcagctgacg
    31141 actggaaagg tgtagagccg atttacgaaa ccatgccggg ctggtctgaa tccactttcg
    31201 gcgtgaaaga tcgtagcggc ctgccgcagg cggcgctgaa ctacatcaag cgtattgaag
    31261 agctgaccgg tgtgccgatc gatatcatct ctaccggtcc ggatcgtact gaaaccatga
    31321 ttctgcgcga cccgttcgac gcgtaattct ggtacgcctg gcagatattt tgcctgccgg
    31381 gctaacagtg tgatacattg ctgtgtcggt taagccatta cgctatccga cacagtgtta
    31441 aatcctcgct tttttccttc cccgaactga aataaattag cgacacagct tgtggctggt
    31501 ttatcatcaa tataaatgta ttttttcccg atttcccttt tgaggttgat gtgcagttaa
    31561 cgagtttcac tgattacgga ttacgtgcgc tgatctacat ggcgtcattg ccagaagggc
    31621 ggatgaccag tatttctgaa gtgactgacg tctacggcgt ctcccgtaat catatggtca
    31681 aaataatcaa tcaacttagt cgtgccggct acgtgactgc tgttcgtgga aaaaatggcg
    31741 gcattcgcct gggtaaaccg gcgagtgcga tacgtattgg tgatgtggtg cgcgagctgg
    31801 agcccttatc gctggtgaat tgcagcagtg agttttgcca cattacacct gcctgtcggt
    31861 tgaaacaggc gctttctaag gccgtgcaaa gttttcttac ggaactggat aactacacgc
    31921 ttgccgattt ggttgaagag aatcaaccgc tttataaatt attgctgttg gagtgacgaa
    31981 aatcttcatc agagatgaca acggaggaac cgagatgtca caagatcctt tccaggaacg
    32041 cgaagctgaa aaatacgcga atcccatccc tagtcgggaa tttatcctcg aacatttaac
    32101 caaacgtgaa aaaccggcca gccgtgatga gctggcggta gaactgcaca ttgaaggcga
    32161 agagcagctt gaaggcctgc gtcgccgcct gcgcgcgatg gagcgcgatg gtcaactggt
    32221 cttcactcgt cgtcagtgct atgcgctgcc ggaacgcctc gacctggtga aaggtaccgt
    32281 tattggccac cgtgatggct acggctttct gcgggttgaa gggcgtaaag atgatttgta
    32341 tctctccagc gagcagatga aaacctgcat tcatggcgat caggtgctgg cgcagccgct
    32401 gggtgctgac cgtaaaggtc gtcgtgaagc gcgtattgtc cgcgtactgg tgccaaaaac
    32461 cagccagatt gttggtcgct actttaccga agcgggcgtc ggctttgtgg ttcctgacga
    32521 tagccgtctg agcttcgata tcttaatccc gcccgatcag atcatgggcg cgcggatggg
    32581 ctttgtggtc gtagtcgaac tgactcagcg tccgactcgc cgcaccaaag cggtgggtaa
    32641 aatcgtcgaa gtgctgggcg acaatatggg caccggcatg gcggttgata tcgctctgcg
    32701 tacccatgaa attccgtata tctggccgca ggctgttgag caacaggttg cggggctgaa
    32761 agaagaagtg ccggaagaag caaaagcggg ccgtgtcgat ttgcgcgatt taccgctggt
    32821 caccattgat ggcgaagacg cccgtgactt tgacgatgca gtttactgcg agaaaaaacg
    32881 cggcggcggc tggcgtttat gggtcgcgat tgccgacgtc agctactatg tgcgtccgcc
    32941 aacgccgctg gacagagaag cgcgtaaccg tggcacgtcg gtgtacttcc cttcgcaggt
    33001 tatcccgatg ctgccggaag tgctctctaa cggcctgtgt tcgctcaacc cgcaggtaga
    33061 ccgcctgtgt atggtgtgcg agatgacggt ttcgtcgaaa ggccgcctga cgggctacaa
    33121 attctacgaa gcggtgatga gctctcacgc gcgtctgacc tacaccaaag tctggcatat
    33181 tctgcagggc gatcaggatc tgcgcgagca gtacgccccg ctggttaagc atctcgaaga
    33241 gttgcataac ctctataaag tgctggataa agcccgtgaa gaacgcggtg ggatctcatt
    33301 tgagagcgaa gaagcgaagt tcattttcaa cgctgaacgc cgtattgaac gtatcgaaca
    33361 gacccagcgt aacgacgcgc acaaattaat tgaagagtgc atgattctgg cgaatatctc
    33421 ggcggcgcgt ttcgttgaga aagcgaaaga accggcactg ttccgtattc acgacaagcc
    33481 gagcaccgaa gcgattacct ctttccgttc agtgctggcg gagctggggc tggagctgcc
    33541 gggtggtaac aagccggaac cgcgtgacta cgcggaactg ctggagtcgg ttgccgaccg
    33601 tcctgatgca gaaatgctgc aaaccatgct gctacgctcg atgaaacagg cgatttacga
    33661 tccagaaaac cgtggtcact tcggtctggc attgcagtcc tatgcgcact ttacttcgcc
    33721 gattcgtcgt tatcctgacc tgacgctgca ccgcgccatt aaatatctgc tggcgaaaga
    33781 gcaggggcat cagggcaaca ccactgaaac cggcggctac cattattcga tggaagagat
    33841 gttgcaactg ggtcagcact gttcgatggc ggaacgtcgt gccgacgaag caacgcgcga
    33901 tgtcgctgac tggctgaagt gtgacttcat gctcgaccag gtaggtaacg tctttaaagg
    33961 cgtaatttcc agcgtcactg gctttggctt cttcgtccgt ctggacgact tgttcattga
    34021 tggtctggtc catgtctctt cgctggacaa tgactactat cgctttgacc aggtagggca
    34081 acgcctgatg ggggaatcca gcggccagac ttatcgcctg ggcgatcgcg tggaagttcg
    34141 cgtcgaagcg gttaatatgg acgagcgcaa aatcgacttt agtctgatct ccagtgaacg
    34201 cgcaccgcgc aacgtcggta aaacggcgcg cgagaaagcg aaaaaaggcg atgcaggcaa
    34261 aaaaggcggc aagcgtcgtc aggtcggtaa aaaggtaaac tttgagccag acagcgcctt
    34321 ccgcggtgag aaaaaaacga agccgaaagc ggcgaagaaa gacgcgagaa aagcgaaaaa
    34381 gccatcggcg aaaacgcaga aaatagccgc agcgaccaaa gcgaagcgtg cggcgaagaa
    34441 aaaagtggca gagtgatcaa taccctcttt aaaagaagag ggttagattg ctgacaaaat
    34501 gcgctttgtt catgccggat gcggcgtgaa cgccttatcc ggcctacata atcacgcaaa
    34561 ttcaatatat tgcagagatc atgtaggccg gataaacgta gtgcatcagg caaaacgtaa
    34621 acaacgagta cattaatgag cgaaatgatt tacggcatcc acgcagtgca ggccctgctg
    34681 gagcgcgccc ctgaacgttt tcaggaagtc tttattttaa aaggccgtga agataaacgt
    34741 ctgttaccgc tgattcacgc ccttgagtct cagggcgtgg ttatccagtt ggcaaaccgc
    34801 caatatctcg acgagaaaag cgacggtgcc gtgcatcagg gcattatcgc ccgcgtgaag
    34861 ccaggacgtc agtatcagga aaacgatctg ccggatctga tcgcttcgct cgatcaaccg
    34921 ttcctgctga tcctcgacgg cgtaaccgat ccgcacaacc tcggcgcgtg cctgcgtagt
    34981 gcggacgccg caggcgttca tgcggtgatt gtgccgaaag atcgctccgc acagctcaac
    35041 gctacggcga aaaaagtagc ctgcggcgcg gcagaaagcg ttccgctgat tcgggtgact
    35101 aaccttgcgc gcaccatgcg tatgttgcag gaagagaata tctggatcgt cggtacggca
    35161 ggcgaggcgg atcatacact ctatcagagc aaaatgaccg gacgcctggc gctggtgatg
    35221 ggcgcggaag gtgaaggtat gcgtcgcctg acccgtgaac attgcgatga gttgatcagc
    35281 atcccaatgg caggaagtgt ttcttccctg aacgtttcgg ttgcaaccgg aatttgctta
    35341 tttgaagcgg ttcgtcagcg cagctagttc ctctgaatta tgaaaagcca tccagatttg
    35401 gatggtt
//
LOCUS       DAETPZ010000035        35272 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.035, whole genome shotgun sequence.
ACCESSION   DAETPZ010000035 DAETPZ010000000
VERSION     DAETPZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.035"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            606..2891
                     /gene="nrdA"
                     /locus_tag="K0W59_001946"
     CDS             606..2891
                     /gene="nrdA"
                     /locus_tag="K0W59_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HBI3022990.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3125..4255
                     /gene="nrdB"
                     /locus_tag="K0W59_001947"
     CDS             3125..4255
                     /gene="nrdB"
                     /locus_tag="K0W59_001947"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBI3022991.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4255..4509
                     /gene="yfaE"
                     /locus_tag="K0W59_001948"
     CDS             4255..4509
                     /gene="yfaE"
                     /locus_tag="K0W59_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HBI3022992.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4563..5213)
                     /gene="inaA"
                     /locus_tag="K0W59_001949"
     CDS             complement(4563..5213)
                     /gene="inaA"
                     /locus_tag="K0W59_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HBI3022993.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSRVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5377..5619
                     /locus_tag="K0W59_001950"
                     /pseudo
     CDS             5377..5619
                     /locus_tag="K0W59_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5661..6737)
                     /gene="glpQ"
                     /locus_tag="K0W59_001951"
     CDS             complement(5661..6737)
                     /gene="glpQ"
                     /locus_tag="K0W59_001951"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBI3022994.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6742..8100)
                     /gene="glpT"
                     /locus_tag="K0W59_001952"
     CDS             complement(6742..8100)
                     /gene="glpT"
                     /locus_tag="K0W59_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HBI3022995.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8373..10001
                     /gene="glpA"
                     /locus_tag="K0W59_001953"
     CDS             8373..10001
                     /gene="glpA"
                     /locus_tag="K0W59_001953"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HBI3022996.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIAHHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9991..11250
                     /gene="glpB"
                     /locus_tag="K0W59_001954"
     CDS             9991..11250
                     /gene="glpB"
                     /locus_tag="K0W59_001954"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HBI3022997.1"
                     /translation="MRFDTVIMGSGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNKENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQITQRAGGQQ"
     gene            11247..12437
                     /gene="glpC"
                     /locus_tag="K0W59_001955"
     CDS             11247..12437
                     /gene="glpC"
                     /locus_tag="K0W59_001955"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HBI3022998.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12630..13529
                     /locus_tag="K0W59_001956"
     CDS             12630..13529
                     /locus_tag="K0W59_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HBI3022999.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDESDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGFEREIVLAATQLTDADIPNCY"
     gene            13533..13769
                     /locus_tag="K0W59_001957"
                     /pseudo
     CDS             13533..13769
                     /locus_tag="K0W59_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13810..14280)
                     /locus_tag="K0W59_001958"
     CDS             complement(13810..14280)
                     /locus_tag="K0W59_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBI3023000.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14335..15537)
                     /locus_tag="K0W59_001959"
     CDS             complement(14335..15537)
                     /locus_tag="K0W59_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HBI3023001.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15624..16166)
                     /locus_tag="K0W59_001960"
     CDS             complement(15624..16166)
                     /locus_tag="K0W59_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023002.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16445..16870
                     /gene="nudI"
                     /locus_tag="K0W59_001961"
     CDS             16445..16870
                     /gene="nudI"
                     /locus_tag="K0W59_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HBI3023003.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16909..17511)
                     /locus_tag="K0W59_001962"
     CDS             complement(16909..17511)
                     /locus_tag="K0W59_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBI3023004.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17819..18958
                     /gene="arnB"
                     /locus_tag="K0W59_001963"
     CDS             17819..18958
                     /gene="arnB"
                     /locus_tag="K0W59_001963"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HBI3023005.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDTIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18962..19930
                     /gene="arnC"
                     /locus_tag="K0W59_001964"
     CDS             18962..19930
                     /gene="arnC"
                     /locus_tag="K0W59_001964"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HBI3023006.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19930..21912
                     /gene="arnA"
                     /locus_tag="K0W59_001965"
     CDS             19930..21912
                     /gene="arnA"
                     /locus_tag="K0W59_001965"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HBI3023007.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYDS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            21909..22799
                     /locus_tag="K0W59_001966"
     CDS             21909..22799
                     /locus_tag="K0W59_001966"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HBI3023008.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22799..24444
                     /gene="arnT"
                     /locus_tag="K0W59_001967"
                     /pseudo
     CDS             22799..24444
                     /gene="arnT"
                     /locus_tag="K0W59_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
     gene            24441..24776
                     /gene="arnE"
                     /locus_tag="K0W59_001968"
     CDS             24441..24776
                     /gene="arnE"
                     /locus_tag="K0W59_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HBI3023009.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24776..25162
                     /gene="arnF"
                     /locus_tag="K0W59_001969"
     CDS             24776..25162
                     /gene="arnF"
                     /locus_tag="K0W59_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HBI3023010.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25156..25428)
                     /gene="pmrD"
                     /locus_tag="K0W59_001970"
     CDS             complement(25156..25428)
                     /gene="pmrD"
                     /locus_tag="K0W59_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HBI3023011.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25533..26888)
                     /gene="menE"
                     /locus_tag="K0W59_001971"
     CDS             complement(25533..26888)
                     /gene="menE"
                     /locus_tag="K0W59_001971"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HBI3023012.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26885..27847)
                     /gene="menC"
                     /locus_tag="K0W59_001972"
     CDS             complement(26885..27847)
                     /gene="menC"
                     /locus_tag="K0W59_001972"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HBI3023013.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFTKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(27847..28704)
                     /gene="menB"
                     /locus_tag="K0W59_001973"
     CDS             complement(27847..28704)
                     /gene="menB"
                     /locus_tag="K0W59_001973"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HBI3023014.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDICYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28719..29477)
                     /gene="menH"
                     /locus_tag="K0W59_001974"
     CDS             complement(28719..29477)
                     /gene="menH"
                     /locus_tag="K0W59_001974"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HBI3023015.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIIEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29474..31144)
                     /gene="menD"
                     /locus_tag="K0W59_001975"
     CDS             complement(29474..31144)
                     /gene="menD"
                     /locus_tag="K0W59_001975"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HBI3023016.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIGNIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31233..32528)
                     /gene="menF"
                     /locus_tag="K0W59_001976"
     CDS             complement(31233..32528)
                     /gene="menF"
                     /locus_tag="K0W59_001976"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HBI3023017.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAKGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGERLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32607..32912)
                     /gene="elaB"
                     /locus_tag="K0W59_001977"
     CDS             complement(32607..32912)
                     /gene="elaB"
                     /locus_tag="K0W59_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HBI3023018.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGEPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32967..33428)
                     /locus_tag="K0W59_001978"
     CDS             complement(32967..33428)
                     /locus_tag="K0W59_001978"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI3023019.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDCIPHIGMAREVIQA"
     gene            33493..34410
                     /gene="rbn"
                     /locus_tag="K0W59_001979"
     CDS             33493..34410
                     /gene="rbn"
                     /locus_tag="K0W59_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HBI3023020.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34598..35272
                     /locus_tag="K0W59_001980"
                     /pseudo
     CDS             34598..35272
                     /locus_tag="K0W59_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8673 a         9421 c         8716 g         8462 t
ORIGIN      
        1 tgcaatggct attttgttat tatttgatga ataatatcag tgcgtcataa ttcaagttaa
       61 taaccttaag ggataccagt tatatttaaa ctaaattaaa gtaatgaata attttcttat
      121 aatataaggt aaattaacaa aatggcttcg catttaacaa taaccgaata gaaaacaacc
      181 atttcgacat caacaatcgc ttacattcgc ttatatattg accacaactg atacatcaga
      241 ttatgtgatg actcgtgctt agatcaattt ttgcaatcat tagcaaaaag attaataaac
      301 catctatatc aatttatcca acctattctg ccgttcaaga aatcgccgaa cagttatttt
      361 taacaaattt ttctcttccc attgactttc ccggacacct tgtctgacct aaggtgcgcg
      421 aaagccactt tttccttcct gagttatcca caaagttatg cacttgcaag agggtcattt
      481 tcacactatc ttgcagtgaa tcccaaacat accccctata tatagtgttc taagcagctt
      541 cccttactac aggtagtctg catgaaacta ttgcggaaag aattccaaaa acaggtacga
      601 catacatgaa tcagaatctg ctggtgacaa agcgcgacgg tagcacagag cgcatcaatc
      661 tcgacaaaat ccatcgcgtt ctggattggg cggcagaagg actgcataac gtttcgattt
      721 cccaggtcga gctgcgctcc cacattcagt tttatgacgg tatcaagacc tctgacatcc
      781 acgaaactat tatcaaggct gccgcagacc tgatctcccg tgatgcgccg gattatcagt
      841 atctcgccgc gcgcctggcg atcttccacc tgcgtaaaaa agcctacggc cagtttgagc
      901 cgcctgcgct gtacgaccac gtggtgaaaa tggtcgagat gggcaaatac gataatcatc
      961 tgctggaaga ctacacggaa gaagagttca agcagatgga cacgtttatc gatcacgacc
     1021 gtgatatgac cttctcttat gctgccgtta agcagctgga aggcaaatat ctggtacaga
     1081 accgcgtgac cggcgaaatc tacgaaagcg cccagttcct ttatattctt gttgccgcgt
     1141 gcctgttctc gaactacccg cgtgaaacgc gcctgcaata cgtgaagcgt ttttacgacg
     1201 cggtttccac atttaaaatt tcgctgccga cgccaatcat gtccggcgtg cgtaccccga
     1261 ctcgtcagtt cagctcctgc gtactgatcg agtgcggtga cagcctggat tccatcaacg
     1321 ccacctccag cgcgattgtg aaatatgttt cccagcgtgc cgggatcggc atcaacgccg
     1381 gacgtattcg tgcgctgggc agcccgattc gcggtggcga agcgttccat accggctgta
     1441 tcccgttcta caaacatttc cagacagcgg tgaaatcctg ctctcagggc ggtgtgcgcg
     1501 gcggtgcggc aacgctgttc tacccgatgt ggcatctgga agtggaaagc ctgctggtgt
     1561 tgaaaaacaa ccgtggggtt gaaggcaacc gcgtgcgtca tatggactac ggggtacaaa
     1621 tcaacaaact gatgtatacc cgcctgctga aaggtgaaga tatcaccctg ttcagcccgt
     1681 ccgacgtacc ggggctgtac gacgcgttct tcgccgatca ggaagagttt gaacgcctgt
     1741 ataccaaata tgagaaagac gacagcatcc gcaagcagcg tgtgaaagcc gttgagctgt
     1801 tctcgctgat gatgcaggaa cgtgcgtcta ccggtcgtat ctatattcag aacgttgacc
     1861 actgtaatac ccatagtccg tttgatccga ccatcgcgcc agttcgccag tctaacctgt
     1921 gcctggaaat tgccctgccg accaaaccgc tgaacgacgt caacgacgag aacggtgaaa
     1981 tcgcgctgtg tacgctgtct gctttcaacc tgggcgcaat taatagcctg gatgaactgg
     2041 aagagctggc gattctggcg gttcgtgcac ttgacgcgct actggattat caggattacc
     2101 cgatcccggc cgccaaacgt ggagcgatgg ggcgtcgtac gctgggtatt ggtgtgatta
     2161 acttcgctta ctatctggcg aagcacggta aacgctactc cgacggcagc gccaacaacc
     2221 tgacgcataa aaccttcgaa gcgattcagt attacctgct gaaagcgtct aatgagctgg
     2281 ctaaagagca aggcgcgtgc ccgtggttta acgaaaccac ttacgcgaaa gggatcctgc
     2341 cgatcgatac ctataagaaa gatctggatg ccatcgctaa tgagccgctg cattacgact
     2401 gggaagcatt gcgtgaatca atcaaaacgc acggcctgcg taactccacg ctttctgccc
     2461 tgatgccgtc cgagacctct tcgcagatct ctaacgccac taacggtatt gaaccgccgc
     2521 gcggttacgt cagcatcaaa gcgtcgaaag acggtatttt gcgccaggtg gtgccggact
     2581 acgagcacct gcacgacgcc tatgagctgc tgtgggaaat gccgggtaac gatggctacc
     2641 tgcaactggt aggtatcatg cagaaattta tcgatcagtc gatctctgct aacaccaact
     2701 acgatccgtc acgtttcccg tcaggaaaag tgccgatgca gcagttgctg aaagacctgc
     2761 tcaccgccta caaattcggg gtcaaaacgc tgtattatca gaacacccgt gacggcgctg
     2821 aagacgcgca ggacgatctg gtaccgtcaa tccaggacga tggctgcgaa agcggcgcat
     2881 gtaagatctg atattgagat gccggatgcg gcgtaaacgc cttatccggc ctacggctcg
     2941 gtttgtaggc ctgataagac gcgccagcgt cgcatcaggc tccgggtgcc ggatgcagcg
     3001 tgaacgcctt atccggccta cggctcggat ttgtaggcct gataagacgc gccagcgtcg
     3061 catcaggcac aggatgcggc gtaaaatgcc ttatccggca ttaaactccc aacaggacac
     3121 actcatggca tataccacct tttcacagac gaaaaatgat cagctcaaag aaccgatgtt
     3181 ctttggtcag ccggtcaacg tggctcgcta cgatcagcaa aaatatgaca tcttcgaaaa
     3241 gctgatcgaa aagcagctct ctttcttctg gcgtccggaa gaagttgacg tctcccgcga
     3301 ccgtatagat taccaggcgc tgccggagca cgaaaaacac atctttatca gcaacctgaa
     3361 atatcagacg ctgctggatt ccattcaggg tcgtagcccg aacgtggcgc tattgccgct
     3421 tatttctatt ccggaactgg aaacctgggt cgaaacctgg gcgttctcag aaacgattca
     3481 ttcccgttcc tatactcata tcattcgtaa tatcgttaac gatccgtctg ttgtgtttga
     3541 cgatatcgtc accaacgagc agatccagaa acgtgcggaa gggatctcca gctattacga
     3601 tgaactgatc gaaatgacca gctactggca ccttctgggc gaaggcaccc acaccgttaa
     3661 cggtaaaacc gtaaccgtta gcctgcgcga gctgaagaaa aaactgtatc tctgcctgat
     3721 gagcgttaac gcgctggaag cgatccgctt ctacgtcagc tttgcttgtt cttttgcctt
     3781 tgcagaacgc gaactgatgg aaggtaacgc caagattatt cgcctgattg cccgcgatga
     3841 agccctgcac ctgaccggta ctcagcatat gctgaatctg ctgcgcagcg gcgcggatga
     3901 tccggagatg gcggaaattg ccgaagaatg taagcaggag tgctatgacc tgtttgtcca
     3961 ggcggcacag caagagaaag actgggcgga ttatctgttc cgtgacggtt cgatgattgg
     4021 tctgaataaa gatattctct gccagtacgt tgaatacatc accaatatcc gcatgcaggc
     4081 agtcggtttg gatctgccgt tccagacgcg ctccaacccg atcccgtgga tcaacacctg
     4141 gctggtgtct gataacgtgc aggttgctcc gcaggaagtg gaagtcagtt cttatctggt
     4201 cgggcagatt gacgcggaag tggacaccga cgatttgagt aacttccagc tctgatggcc
     4261 cgcgttaccc tgcgcatcac tggcacacaa ctgctgtgcc aggatgaaca cccttccctt
     4321 ctggcggcgc tggaatccca caatgtggcg gttgagtacc agtgccgcga aggttactgc
     4381 ggctcctgtc gcacccgact ggtcgcaggt caggtggact ggattgccga accgttagcc
     4441 tttattcagc cgggggaaat tttgccctgt tgttgccggg caaaaggcga tattgaaatc
     4501 gagttgtgat cgggtactag cgccagatac gacgcttacg cgtattatct ggcctacaac
     4561 gattacatgg cgtagtaata cgctttcacc tgctcccagt ccgctttagg gatcggctcc
     4621 agatattttt ccagctggcg gaagtcatga ttaatcgctt tatcgcgacg caagcggcga
     4681 cgacttttct ccagatcaag aaaaccagct tctgccttac cttctgtttt cacatagata
     4741 tggcgaacat agcaacagcc atgctgacga ttaacgctat gcattttctt aaacgccagt
     4801 gccactgctt tcaacatggc ttgccgtact tcgtcagaat aaggcgatac tgcatgccgg
     4861 gcataccagt cagcaatgct gatgaacccc gccatatctt cagtcaccag caacgcgcgc
     4921 cattcaccct caattttcac cgcttcacca aaaacgatct tcggcacgat gacaccggcc
     4981 cgttccagtt ctttaattac cgcaacctca cggacaatcg ttggtcggcc gaacggataa
     5041 cgtactgaat gaaacagatg atgcgtcatg cgctttacat acagcttttt gccgttgcgc
     5101 tcgacgcatt gcaccctgct cataccatta cggcgatagt taggctcttc aacccagtcg
     5161 ccctctgttg cccaccagtg attaaattcg tcgtactttg ctgaaactgc cataccctat
     5221 cgcctgtcat ttttattaac gacaatgact ataggtggtt acctgaggaa aatcttaatg
     5281 aaacgtgtcg tattccgcta caggtgtgac accgcttttg cccttcattt gcagcgcata
     5341 gattccacca gccactacca ctaatagtgc gcccagcaga taaaatgtga atttaatcag
     5401 tcggatacag cgatgaattt tattcgacag ggattaggca tcgccttaca accagagtta
     5461 acgctgaaaa gcattgcagg tgaattgtgt tccgttcctc acgaaccaac cttctatcga
     5521 cagatttcgt tgctgactaa agaaaagccg gtagaaggca gtccactgtt tttactacaa
     5581 atgtgcatgg aacaattagt ggcgattgga aaaatttgat atgagacagg atggcgcatg
     5641 aacgccatcc tgcagtaaca ttactcttta ttaagaaatt ttactgcctt atcagggaaa
     5701 tcagtaaaca gcccatttac acccgctttg ttatacagag catcatataa ctgattcaca
     5761 tcaggagtgt attcaggcag tttatctgac cgcacggtat aaggatgcac taccagttta
     5821 ttctgctgag catcttgcac catgccagtg agtttgatat tacccggctg cgatgtctcc
     5881 tcaatcaaca tatggtaatc cggaccaata ccatctgcat attccgccac ctgtttcatg
     5941 gcacccggct taaacatcca gtcgtagttg taattaaccc agcttccatc cggctgtttc
     6001 tgctgcgttt cattccagtc ggtataggca atcagctgta ccagattgag ctccatgccc
     6061 attttgggtt ccagctcatt cttaatacgc ttcagctcat cagcatcaaa acattgcaaa
     6121 taaactttat cgtctttacc ggtgtaacca tatttcttca gcacttccag cgtttttgcc
     6181 gcaatatcct tcccttcctg atgatggaac cacggcgctt tgatttctgg atagataccg
     6241 atatttttcc cggtagagtg atttaacccc tgaacaaatt caatctcttc ttcaaaggtg
     6301 tgcacccgga agtcggactt acccattggg aaacgccccg gataagtctg tactttttta
     6361 ccgttttcaa tatcgaaacc ttcggtaaat ttcagcgact taatttcatc cagcgtgaaa
     6421 tctatcgcgt agtaacgacc gtctttgcgc gcccgatccg ggaaacgatc ggcaacatca
     6481 gtaacacgat cgagataatg gtcatgcaga acaaccagat ggtcgtcttt ggtcatcacc
     6541 aaatcctgtt ccagataatc cgctccctgc gcatacgcca tcgcttttgc tggcagcgta
     6601 tgctccggca aatatccact ggcaccgcga tgggcgatga ctattttttc gttgctgtcc
     6661 gccgccattg cactgcttcc catgactata gtgctcatca tgatcgccat gctaaggttt
     6721 ttcagcttca atttcatgcc attagcctcc gttgcgtttt tgcagtaatt gttcatggcg
     6781 acgtttttcg ccaatcatca caacaatcaa caagataacc gccagaatgc tgccgccaat
     6841 cattaccata aagccgccat cccagccgaa gaagtccacg gtgtagccaa caatcgcgct
     6901 cgccgccacc gaaccgccca ggtaaccaaa cagcccggta aagcccgctg ccgtacctgc
     6961 cgcttttttc ggtgccagtt ccagcgcatg cagaccgatc agcatcacag gaccgtagat
     7021 caggaagccg ataacaatca tacaaatcat atcgacggtt gggttacctg ccgggttcat
     7081 ccagtaaacg atagtcgcga tggtcaccag tgtcataaag aagacgccgg ttgccccacg
     7141 gttaccacgg aagactttgt ccgacatcca gccgcacagc agggtgcccg gaatacccgc
     7201 atactcatag aagaagtagg cccaggagga tttatccagc gcgaaatgct taacctcttt
     7261 cagataagtc ggtgaccagt cgaggatgcc gtaacgcagc agataaacga acacgttggc
     7321 gatggcgatg taccacagca gtttgttcgg cagcacgtac tgcatgaaga tttgcttcgc
     7381 ggtcagctcc tgttccgctt tttcgttata gtcgtccgga taatcatttt tgtactcttc
     7441 gatcggcggc aagccacagg attgcggggt atcgcgcatc atcgcaaagg cgaataatgc
     7501 caccagaatg gcgcagaaag caggcatata gagcgccgca tgccagtcat tgaaccaggc
     7561 catccccagc aggaacagca gcggcggaat accaccaccg acgttgtgcg cacagttcca
     7621 cactgacaca atgccgccac gttctttctg cgaccaccag tgcaccatag tacgaccaca
     7681 cggcggccac cccatcccct ggaaccaacc gcagaggaac aacagtacaa acatcaccgc
     7741 aatgctcgac gtcgcccatg gcacaaagcc cataaacaac atcactgccg ccgccagaat
     7801 caaacctgcg ggcaggaaaa cgcgcggatt cgagcgatcc gataccgaac ccatgatgaa
     7861 tttcgaaaat ccataagcaa tcgagatccc cgaaagggca aaacctaaat caccgcgtga
     7921 gaatccctgc tcaaccagat aaggcatagc aagcgcaaag ttcttacgaa ccaaatagta
     7981 agccgcatag ccaaagaata tccccaggaa aatttgccag cgcaatcgac gataagtcgg
     8041 atcgatctcc gcggcaggta agcgcgcttt gtgtggcgct ggtttaaaaa tactcaacat
     8101 tgatagcctc cgtggcccgt ggtcttattt atgattaaca gcctgattca gtgagagaac
     8161 ctgccgtttc ttgagttgcc gcgatgttaa gaaaacattc ataaattaaa tgtgaattgc
     8221 cgcacacatt attaaataag atttacaaaa tgttcaaaat gacgcatgaa atcacgtttc
     8281 actttcgaat tatgagcgaa tatgcgcgaa atcaaacaat tcatgttttt actatggcta
     8341 aatggtaaaa aacgaacttc agagggataa caatgaaaac tcgcgactcg caatcaagtg
     8401 acgtgattat cattggcggc ggcgcaacgg gagccgggat tgcccgcgac tgtgccctgc
     8461 gcgggctgcg cgtgattttg gttgagcgcc acgacatcgc aaccggtgcc accgggcgta
     8521 accacggcct gctgcacagc ggtgcgcgct atgcagtaac cgatgcggaa tcggcccgcg
     8581 aatgcattag tgaaaaccag atcctgaaac gcattgcaca tcactgcgtt gaaccaacca
     8641 acggcctgtt tatcaccctg ccggaagatg acctctcatt ccaggccact tttattcgcg
     8701 cctgcgaaga agcagggatc agcgcagaag ctatagaccc gcagcaagcg cgcattatcg
     8761 aacctgccgt taacccggca ctgattggcg cggtgaaagt tccggatggc accgttgatc
     8821 catttcgtct gaccgcagca aacatgctgg atgccaaaga acacggtgcc gttatcctta
     8881 ccgctcatga agtcacgggg ctgattcgtg aaggcgcgac ggtgtgcggt gttcgtgtac
     8941 gtaaccatct caccggcgaa actcaggccc ttcatgcacc tgtcgtggtt aatgccgctg
     9001 ggatctgggg gcaacacatt gccgaatatg ccgatctgcg cattcgcatg ttcccggcga
     9061 aaggatcgct gctgatcatg gatcaccgca ttaaccagca tgtgatcaac cgctgccgta
     9121 aaccttccga cgccgatatt ctggtgcctg gcgataccat ttcgctgatt ggtaccacct
     9181 ctttacgtat tgattacaac gagattgacg ataatcgagt gacggcagaa gaggttgata
     9241 ttctgctgcg tgaaggggaa aaactggccc ccgtgatggc gaaaacgcgc attttgcggg
     9301 cctattctgg cgtgcgcccg ctggttgcca gcgatgacga cccgagcgga cgtaacgtca
     9361 gccgtggcat cgtgctgctc gaccatgctg aacgcgatgg tctggacgga tttatcacca
     9421 tcaccggtgg caaactgatg acctatcggc tgatggctga atgggctacc gacgcggtat
     9481 gccgcaaact gggcaacacg cgcccctgta cgactgccga tctggcactg cctggttcac
     9541 aagaacccgc tgaagttacc ttgcgtaaag tcatctccct gcctgccccg ctgcgcggtt
     9601 ctgcggttta tcgtcatggc gatcgcacgc ctgcctggct gagcgaaggc cgtctacacc
     9661 gtagcctggt atgtgagtgc gaagcggtaa ctgcgggtga agtgcagtac gcggtagaaa
     9721 atttaaacgt taatagcctg ctggatttac gccgtcgtac ccgtgtgggg atgggcacct
     9781 gccagggcga actctgcgcc tgccgcgctg ccggactgct gcaacggttt aacgtcacga
     9841 cgtccgcgca atctatcgag caactttcca ccttccttaa cgaacgctgg aaaggcgtgc
     9901 aacccatcgc ctggggagac gcactgcgcg aaagcgaatt tacccgctgg gtttatcagg
     9961 gactgtgtgg tctggagaag gagcagaaag atgcgctttg atactgtcat tatgggcagc
    10021 ggcctcgccg gattactctg tggcctgcaa ctgcaaaaac acggcctgcg ctgtgccatt
    10081 gtcacgcgtg gtcaaagcgc gttgcatttc tcatccggtt cgctggattt gctgagccat
    10141 ctgccagatg gtcaacccgt ggcagacatt cacagtggac tggaatcttt gcgtcagcag
    10201 gcacctgcac atccttactc tcttctcggg ccacaacgcg tgctcgatct cgcttgccag
    10261 gcgcaggcat taatcgctga aagcggtgcg caattgcagg gcagcgtaga actggctcac
    10321 cagcggatta cgccgctcgg cactttgcgt gcaacctggt taagttcgcc agaagttccc
    10381 gtctggccgc tacccgcgaa gaagatatgt gtagtgggga taagcgggct gatggatttc
    10441 caggcgcatc ttgcggcggc ttcgttgcgc gaactggatc tttcggttga aaccgcagaa
    10501 atagagctgc cggaactgga tgtcctgcgc aataacgcca ccgaatttcg cgcggtgaat
    10561 atcgcccgtt tccttgataa taaagaaaac tggccgctgt tacttgatgc gcttattcct
    10621 gtcgccaata cctgcgaaat gatcctgatg cccgcctgct tcggcctagc cgatgacaaa
    10681 ctgtggcgtt ggttgaatga aaaactacct tgttcactga tgcttttgcc aacgctgccg
    10741 ccttccgtgc tgggcattcg tctgcaaaac cagttacagc gccagtttgt gcgccagggt
    10801 ggagtgtgga tgccgggcga tgaagtgaaa aaagtgacct gtaaaaatgg cgtagtgaat
    10861 gaaatctgga cccgcaatca cgcagatatt ccgctacgtc cacgtttcgc ggttctcgcc
    10921 agcggcagtt tctttagtgg cggactggta gcggaacgta acggcattcg cgagccgatt
    10981 ctcggccttg atgtgctaca aaccgccacg cggggtgaat ggtataaggg cgattttttt
    11041 gcgccgcaac cgtggcagca gttcggtgta accactgatg agacgctacg cccgtcgcag
    11101 gcagggcaaa ccattgagaa cctgtttgcc atcggttcgg tgctgggcgg atttgatccc
    11161 atcgcccagg gatgcggcgg cggtgtttgt gccgtcagtg ctttacatgc cgctcaacag
    11221 attacccaac gcgcaggagg ccaacaatga acgacaccag cttcgaaaac tgcattaagt
    11281 gcaccgtctg caccaccgcc tgcccggtga gccgggtgaa tcccggttat ccagggccaa
    11341 aacaagccgg gccggatggc gagcgtctgc gtttgaaaga tggcgcactg tatgacgagg
    11401 cgctgaaata ttgcatcaac tgcaaacgtt gtgaagtcgc ctgcccgtcc gatgtgaaga
    11461 ttggcgatat tatccagcgc gcgcgggcga aatatgacac cacgcgcccg tcgctgcgta
    11521 attttgtgtt gagtcatacc gacctgatgg gtagcgtttc cacgccgttc gcaccaatcg
    11581 tcaacaccgc tacctcgctg aaaccggtgc ggcagctgct tgatgcggcg ttaaaaatcg
    11641 atcatcgtcg cacgctaccg aaatactcct tcggcacgtt ccgtcgctgg tatcgcagca
    11701 tagcggctca gcaagcacaa tataaagacc aggtcgcttt ctttcacggc tgcttcgtta
    11761 actacaacca tccgcagtta ggtaaagatt taattaaagt gctcaacgca atgggtaccg
    11821 gtgtacaact gctcagcaaa gaaaaatgct gcggcgtacc gctaatcgcc aacggcttta
    11881 ccgataaagc acgcaaacag gcaattacga atgtagagtc gatccgcgaa gctgtgggag
    11941 taaaaggcat tccggtgatt gccacctcct caacctgtac atttgccctg cgcgacgaat
    12001 acccggaagt gctgaatgtc gacaacaaag gcttgcgcga tcatatcgaa ctggcaaccc
    12061 gctggctgtg gcgcaagctg gacgaaggca aaacgttacc gctgaaaccg ctgccgctga
    12121 aagtggttta tcacactccg tgccatatgg aaaaaatggg ctggacgctc tacaccctgg
    12181 agctgttgcg taacatcccg gggcttgagt taacggtgct ggattcccag tgctgcggta
    12241 ttgcgggtac ttacggtttt aaaaaagaga actaccccac ctcacaagcc atcggcgcac
    12301 cactgttccg ccagatagaa gaaagcggcg cagatctggt ggtcaccgac tgcgaaacct
    12361 gtaaatggca gattgagatg tccacaagtc ttcgctgcga acatccgatt acgctactgg
    12421 cccaggcgct ggcttaaact cccttctgat gcccggtaag catgtggtta ccgggcattt
    12481 ttgcgtacac gattccgtgc ccaatgtatg cgttgcaacg cagtgaaaat tcctctgaaa
    12541 acgtctcgca aaggctgaaa ctggcagatg tcaaaggcct gagataaccg taatgtcgcg
    12601 tcatcataaa tatcaggtga cggacaacca tgaccgaatc aacaacctcc tccccgcatg
    12661 atgcggtatt taaaaccttt atattcacac ccgaaaccgc acgggatttt ctcgaaatac
    12721 atttaccaga accactgcgc aagctttgca acctgcaaac cttacgcctg gaacccacta
    12781 gttttattga aaaaagttta cgcgcttact actcggatgt tttgtggtcc gtggaaacca
    12841 gcgacggtga cggctatatc tactgcgtga ttgaacatca aagctctgca gaaaagaata
    12901 tggcttttcg gccaatgcgc tatgccactg ccgccatgca gcgtcaccta gataaaggct
    12961 atgacagagt tccgctggtg gtgccattgc tgttttatca tggcgaaaca tcgccctacc
    13021 cgtactcact taactggctg gatgagtctg acgatccgca acttgcccgg cagttgtaca
    13081 ccgaagcttt tccgttggtg gatatcacca tcgtacctga cgatgagatc atgcaacatc
    13141 ggcgtatagc tctgctggaa ctgattcaaa agcatattcg cgaccacgat ttaatcggca
    13201 tggtcgacag gatcaccacg cttttggtta gaggcttcac taatgacagc cagctacaaa
    13261 cactgtttaa ttatctgctg caatgcggcg atacctcccg tttcacccgt tttattcagg
    13321 agattgccga acgttcacca ctacaaaagg agagattaat gactattgct gaacggctac
    13381 ggcaggaagg gcatcaaatt ggctggcagg aaggtatgca tgaacaagcc attaaaattg
    13441 ccttgcgcat gctggagcag ggctttgaac gtgagattgt gctggcggca acccaactca
    13501 ctgatgctga tattccgaac tgttattaaa aggagaggga gagattgatg actattgctg
    13561 aacggctacg gcaggaagga catcaaattg gctggcaaga aggtaaatta gtaggtttgc
    13621 agcaaggtaa attagaaggt ttgcagcaag gtatgcatga acaagccatt aaaattgctt
    13681 tgcgcatgct ggaacagggc attgatcgtg accaagtgct cgcggccacc cagctaagcg
    13741 aagccgatct ggcagcgaat aaccactaat taacacaggc ccacagccga tccccatggg
    13801 cctttgatat caataactac cttttatgcg tggcccattt ttgcctgatt taaacatcgc
    13861 cagtcgttga tccagggcat cgctgtagag catcgtgtca acgccaacag cgacaaagtt
    13921 cgctccccac gccaggcatt gctgcgccat atcaggagcc acagccagaa aaccagccgc
    13981 tttacccgca gcacggatcc gccgaatgct ggtttcaata attcgctgca cttccgggtg
    14041 cccggcgtta tccgggtagc ccaacgacgc agaaagatcc gctggtccaa taaacacgcc
    14101 atcaatccct tcgacgtcga ggatctcgtc caggttatcc agtgccgttt tactttccac
    14161 ctgcactaac agacaaagcg aatcgttgac ttgcgccatg taattctcaa tgcgtcccca
    14221 gcgcgcagcc cgtgccacac tggccccaac accacgctca ccgtagggag ggttggacaa
    14281 attttgccgg agggggaatg tgccggtgca gtaaaccggc acagagtggc gaaatcagac
    14341 ggataaagtc atggactcaa caacctcaat ccagccatgc tcactagcga tgtcctggcc
    14401 gtttaaccaa cggcgtagca tattcagtgc catcattgcg cacacttcct gacggatagc
    14461 caggctgtag cgtgtagtgc tgaaacgcac acgcagagcg aaagtgccgt ctggcgtggc
    14521 tagcgcaaag ttgagatgct cgttctcgaa acccgaaaca gccagtgcca gcccggcaaa
    14581 atggttggcc cgccgttctg taatccagtg cgcagtttgc gccagggttt cttcctgtga
    14641 aggaatcact tcacacgcca gcaatggagc acctgcgcga gaaagttgca aagccaataa
    14701 accaccggtg aattgctcgc tcaacgtcag gctgaactgg cggttttgca attcgcgact
    14761 gatctgcgcg ggcagtcctt cggtgccttc gaaaatcacg ctctgtccgg caacacgttt
    14821 aacgtccagc cacagttttt ccatcgcctg ttgctcgctt gccggtccgg tgagtttcag
    14881 ttcgatgatc ggcattgagg agcgatagcc cattgttacg cccggcggca gttgtagagt
    14941 gtccaggctt tgtgccagat cgctttccga acgaccaaag gtagttaaac gcagacaaac
    15001 cggcggctgt ggtaaagaaa aacgctcgcg caggcgcggc aggatttcgt gctcgaccat
    15061 caccttaaat tctgacggta cgccgggagt aaagaacatc aggcaacgat taagctgcac
    15121 ggcaaaacca caggcggtgc ctaccgggtt attgataaat tcagcactgg caggcagctc
    15181 cgcttgttta cggttgctcg gtgccattac tcgtccacgt tcgtggaaat agcgttccat
    15241 ctctttgagc caggcttcat gcagcacaag gccttcacct tttgctgtcg cagcggcgag
    15301 tgcgcttaaa tcatcgctgg tcggtcccag cccgccgtta acgatcagca catcggcgtg
    15361 ctgactacgt tcgcgaagaa tggtgactaa gtcatcaagg ttatccccca ccgtattgcg
    15421 gcgagataat ggcagcccct gatgaaagaa aaaatcggcc agccaggcag cgttagtgtc
    15481 aacgatttgc ccgtataaca cttcatcccc ggtggatagc atttccactt ttaacatggt
    15541 tatctcctgc ttttgttatc gaatcactat aacgcaaagg gagataaggc gtagtaagaa
    15601 actgaatgcc gcgccggaag ggatcagaaa ctggcgttaa ccccaacata caggccgtca
    15661 gccacagcgt tgtcgcggtt accgtcttta cccgacagat tcaggtagcg ataacccgcc
    15721 tcaatactga ctggacgcat aatggtgtaa cgcgcgccag cattcgcttc ctcataactt
    15781 tgaataccgc tggagagcga atccggagag tagtaatact cgccaaacaa acggaagctg
    15841 ttgccaattt tccactgcaa accacctcct accgctgcgg cataaccttc atcgccgtaa
    15901 ttcgggttgg tgtacacgcc ttttccgcca acggtcgcca ttaacggccc gagaggcaga
    15961 ttcaacccca gccccacgcc cgcgacgtca ccgtcgtcgt cgttatgtgt ccagttaccg
    16021 cttaaagcca ggcccgtcga ttcagtccca aaaccgacac caatattggt gtattcttta
    16081 cccgcctggc cgctgatgct cattgcattg accgatgcag aaaccaacag cattccggcc
    16141 agacctgcta gcgcaatttt tttcattatc ccctttccca caagcaatta atcaaaattc
    16201 ccaaagctgc gggattgtac tgcctggcac agaggattca atgagcgaaa aaagggatct
    16261 agtcacttat tgtaactaaa tacttcttaa ataattaaac gccgaaaaaa taaacacact
    16321 ttaccgatac acacaaaaat aataataagt gcagttatca ttgcaaaaga gattactgtc
    16381 actttcacat atttctgagt taccgtattc tcatggtttc tttttattta taaggaatac
    16441 ccctgtgcga caacggacta ttgtatgccc tttgattcaa aatgatggtg cttatttgct
    16501 gtgcaaaatg gccgacgatc gcggcgtttt ccccggtcaa tgggcgcttt cgggtggcgg
    16561 cgtggagtct ggcgaacgaa ttgaagaggc actacgccgc gaaattcgcg aagaactggg
    16621 agaacagctg cttttgacag aaatcacgcc gtggaccttc agcgatgata ttcgcaccaa
    16681 gacatatgca gatggtcgca aggaagagat ttatatgatt tacctgattt ttgactgcgt
    16741 ttctgccaac cgagaagtga aaataaacga agagtttcag gactacgcgt gggtaaaacc
    16801 tgaagatctg gtgcattatg atttaaatgt tgccacccga aaaacgttac gtttgaaagg
    16861 tcttctttaa caatgatgac agccatcact ggctgtcaga ttgaaaattt agtggttaac
    16921 gaattcccca tccagataaa ctttgccttt ttccacatgc atgactaaac catccagata
    16981 atcaggttta aatatcgcat cacgcttatt tttagcaata tatgtcaggc aatgattatg
    17041 ggtgaaaata acgatatttt tatcaggcgc tttgctttgt aagtccttaa ttgcactata
    17101 aatctcatta ccgcactgaa gaagtcgttt atctaccgtc agctttttac ccgcggaaaa
    17161 ccaggtcgcc gactgaatgg tccggacggt attactggaa taaagatcga aatcagggat
    17221 gtcagcgcta aaagcgttgc ccagttcacg ggcatcctgg gtacctttaa ccgtaatacc
    17281 tgttttatct gacaagcatt gattggttga acgatcgcaa cgttcagcat ggcgaaacaa
    17341 aacgacaacc gggtgctgct gtgccagtct ggccaatgtt ttattgtcga tacgtggcaa
    17401 accattgcta ctccaggcgg catgagtacc cagtccggca attgcagcga gcgccagtaa
    17461 aatgatgaaa tatttttttg acttcaatga tgagcggcaa aaagctaaca cgtaaactcc
    17521 acctgtagac aagcgcaacc agacaattac cgtgaaattg agcgacattt ctggcgataa
    17581 ttagcagttg gtgtaatatt aaaaatccta cgatgtcggc aaaatgcctc aaatttttgc
    17641 caaatgcaaa gcctaaataa gaaaaaatat aaaaatttca atatttacgt ctaatattag
    17701 tttcttaagg ttaagttaat attctatcct taaaatttcg ctccaaatgg caaaatatac
    17761 acaacactct ttatagcaaa tataagtgga caggtattca atggcggaag gaaaagcaat
    17821 gtcagaattt ttgccttttt cgcgaccagc aatgggcgtg gaggaactcg ctgcagttaa
    17881 agaggttctc gaatccggtt ggatcacaac cggtccgaag aatcaggcgc ttgagcaagc
    17941 cttttgccag ttgacgggaa atcagcatgc catcgcggtc agttcagcca ccgccggaat
    18001 gcatatcacg ctaatggcgt tggaaattgg caagggcgat gaagtgatta cgccttccct
    18061 gacctgggtt tcaaccctca atatgatttc cttgctgggt gcaacgccgg taatggtgga
    18121 tgtcgaccgc gatacgctga tggtcacgcc tgaagcgatc gaatcagcca ttacgccacg
    18181 cactaaagcc atcattccgg tgcattatgc cggtgcgcca gcagatattg acaccattcg
    18241 cgccattggc gaacgttacg gcatcgcagt tatcgaagat gctgcccatg ccgtcggtac
    18301 gtattacaaa gggcgacata ttggcgcaaa aggtaccgct attttttcat ttcatgccat
    18361 taaaaatatt acctgtgctg aaggtggcct gattgtaact gataatgaaa accttgcccg
    18421 ccagctacgg atgctgaaat ttcacggtct gggtgtcgat gcctatgaca gacaaacctg
    18481 gggccgtgca ccgcaggctg aagtcttaac accgggctat aagtacaatc tgaccgatat
    18541 taacgccgcg attgccctga cacagttagt caaattagag cacctcaaca cccgtcggcg
    18601 cgaaattgcc cagcaatatc agcaagcact ggcagctctc ccctttcagc cattaagcct
    18661 tcccgcctgg ccgcacgttc acgcctggca tctgtttatt attcgtgtcg atgaacaacg
    18721 ttgtggtatc agtcgcgatg cgttgatgga agcgttaaaa gaaagaggca ttggtaccgg
    18781 gttacatttc cgcgccgctc acacacaaaa atattatcgc gagcgttttc ccacgctgtc
    18841 gttaccgaat accgaatgga atagcgaacg catctgttcg ttgccgctgt tcccggatat
    18901 gactaccgcc gatgccgacc gcgtcatcac agcccttcag caactcgcag gacaataagc
    18961 catgtttgaa atccaccctg ttaagaaagt ctcggtggtt attcccgttt ataacgagca
    19021 ggaaagctta ccggaattaa tcaggcgcac caccacagcc tgtgaatcgt tggggaaaga
    19081 gtatgagatc ctgctgattg atgacggcag tagcgataat tccgcgcata tgctggtcga
    19141 agcctcacaa gcggagaaca gccatattgt gtctattttg cttaaccgca attacgggca
    19201 acattcagcg attatggcgg gattcagtca cgttactggc gacttaatta ttacccttga
    19261 tgccgatctc cagaatccgc cagaagaaat cccccgcctg gtggcaaaag ccgatgaagg
    19321 ttacgacgtg gtagggactg tacgccagaa ccgccaggac agctggtttc gtaaaacagc
    19381 ttcgaagatg attaaccggc ttattcagcg caccactggc aaagcgatgg gtgactacgg
    19441 ttgtatgctg cgcgcctatc gccgtcatat tgtcgatgcg atgttgcact gccatgaacg
    19501 cagcaccttt atcccgattc tggcgaatat cttcgcccgc cgtgcaattg aaattccagt
    19561 acatcatgcc gagcgtgagt ttggtgaatc caaatacagt tttatgcgcc tgattaattt
    19621 gatgtacgac ctggtgacct gccttaccac aacgccgcta cgtatgctga gtctgctcgg
    19681 cagcattatt gcgattggag gttttagcat tgcggtgctg ctggtgattt tacgcctgac
    19741 cttcggacca caatgggcgg cagaaggcgt ctttatgcta tttgccgtgc tgtttacttt
    19801 tattggcgct cagtttatcg gcatgggatt actcggtgaa tatatcggca ggatctacac
    19861 cgatgtccgc gcccgccccc gctattttgt tcagcaagtt atccgtccat ccagcaagga
    19921 aaatgaataa tgaaaaccgt cgtttttgcc taccacgata tgggatgcct cggtattgaa
    19981 gctctgctgg ctgccggtta cgaaattagc gccattttta cccataccga taatcccggt
    20041 gaaaaagcct tttatgattc ggtggctcat ctggcggcgg aaagagacat tccggtttat
    20101 gcgccggata acgttaatca tccgctgtgg gtggaacgca ttgcccaact gtcaccagag
    20161 gtgattttct ctttttatta tcgccatctt atttgcgacg agatttttca gctcgctccc
    20221 gcaggtgcat ttaatctgca cggttcgctg ttaccaaaat atcgtggtcg cgcgccgctg
    20281 aactgggtgc tggtgaacgg tgaaacggaa acgggcgtta cattgcaccg aatggtaaaa
    20341 cgtgccgatg ctggggccat tgtggcgcaa ctgcgcgttg ccattgcgcc agacgatatc
    20401 gctattacgc tgcatcataa attgtgccat gccgcgcgcc agctactgga acagacatta
    20461 cccgccatta aacacggtaa tattctggaa atcgcccagc gcgaaaacga agccacctgt
    20521 tttggtcgca gaacgccgga tgacagtttc cttgaatggc ataaaccggc atccgtactg
    20581 cacaacatgg tacgtgccgt tgccgatccg tggccgggtg ccttcagcta tgttggcaat
    20641 cagaaattca ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg
    20701 agcgtgattt ctgttgcgcc actgctgatt gcctgtggcg atggcgcgct ggaaatcgtc
    20761 accggacagg cgggcgacgg cattactatg cagggctcgc aattagcgca gatgctgggc
    20821 ctggtgcaag gttcacgctt gaatagccag cctgcctgca ccgcccgacg ccgtacccgg
    20881 gtactcatcc tcggggtgaa tggctttatt ggcaaccatc tgacagaacg cctgctgcgc
    20941 gaagatcatt atgaagttta cggtctggat attggcagcg atgcgataag ccgttttctg
    21001 aatcatccac attttcactt tgtcgaaggc gatatcagta ttcattccga atggattgag
    21061 tatcacgtca aaaaatgtga tgtcgtcttg ccgctggtgg cgatagccac gccgattgaa
    21121 tatacccgca acccgctgcg cgtatttgaa ctcgattttg aagagaatct gcgcattatc
    21181 cgctactgcg ttaagtaccg taagcgaatc atcttcccgt caacttcaga agtttatggg
    21241 atgtgtagcg ataaatactt cgatgaggac cattctaatt taatcgtcgg cccggtgaat
    21301 aaaccacgct ggatttattc ggtgtcaaaa caattacttg atcgggtgat ctgggcctat
    21361 ggcgaaaaag agggtttaca gttcaccctc ttccgcccgt ttaactggat gggaccacga
    21421 ctggataacc ttaatgcagc gcgaattggc agctcccgcg ctattacgca actcattctc
    21481 aatctggtag aaggttcacc aattaagcta attgatggcg gaaaacaaaa acgctgcttt
    21541 actgatatcc gggacggtat cgaggcgttg taccgcatta tcgaaaatgc gggaaatcgc
    21601 tgcgacggcg aaattatcaa cattggcaat cctgagaacg aagcgagcat tgaggaactg
    21661 ggcgagatgc tgctggcgag cttcgaaaaa catccgctgc gccatcattt cccaccgttt
    21721 gcgggctttc gcgttgtcga aagtagcagc tactacggca aaggatatca ggacgtagag
    21781 catcgtaaac cgagcatccg caatgcccac cgctgcctgg actgggagcc gaaaattgat
    21841 atgcaggaaa ccatcgacga aacgctggat ttcttcctgc gcaccgttga tcttacggat
    21901 aaaccatcat gaccaaagta ggcttacgca ttgatgtcga tacctttcgt ggcacccgtg
    21961 aaggcgtgcc gcgtctgctg gaaatcttga gtaagcataa tattcaggcc agcatttttt
    22021 tcagcgtcgg cccggacaat atgggccgcc atctctggcg actggtgaag ccacagtttt
    22081 tgtggaagat gctgcgctca aacgcggcat cgctttatgg ctgggatatt ttactggcag
    22141 gtacggcctg gccaggtaaa gagattggtc atgccaatgc cgatatcatt cgtgaagcgg
    22201 ctaaacatca cgaagtcggt ctgcacgcct gggatcacca tgcctggcaa gcccgtagcg
    22261 gtaactggga tcggcaaaca atgatcgacg atattgcacg cggtcttcgc actctggaag
    22321 agattatcgg tcaaccggta acctgttctg ccgctgcggg ctggcgtgcc gaccagaagg
    22381 tgatcgaagc aaaagaagcg ttccatttgc gctacaacag cgattgtcgt ggggccatgc
    22441 cgttccgtcc actgctcgaa tcaggaaacc ctggcactgc gcaaattccg gtgaccttac
    22501 ccacctggga tgaagtgatt ggtcgggatg tgaaagcaga agattttaac ggttggttac
    22561 tcaaccgcat cctgcgagat aaaggcacgc cggtttatac cattcatgca gaagttgaag
    22621 gctgcgctta tcagcataat tttgtggatc tcctcaaacg cgcagctcag gaaggcgtga
    22681 cattttgccc tttaagcgaa ctgttatcag agacgttgcc gctcggacaa gttgttcgcg
    22741 gaaatattgc cggacgtgaa ggctggctgg gttgccaaca aattgcgggt agtcgctgat
    22801 gaaatcggta cgttacctta tcggcatctt cgcgtttatt gcctgctatt acctgttacc
    22861 gatcagcacg cgtctgctct ggcaacccga tgaaacgcgt tatgcggaaa tcagtcgaga
    22921 aatgctggca tccggcgact ggattgtgcc ccatctgtta gggctacgtt atttcgaaaa
    22981 acccattgcc ggatactgga ttaacagcat tgggcaatgg ctatttggcg cgaataactt
    23041 tggtgtgcgg gcaggcgtta tctttgcgac cctgttaact gccgcgctgg tgacctggtt
    23101 tactctgcgc ttatggcgcg ataaacgtct ggctctactc gccacagtaa tttatctctc
    23161 attgtctatg ccatcggcac ttatgccgtg ctcgatccgt ttattgcatt ctggctggtg
    23221 gcgggaatgt gcagcttctg gctggcaatg caggcacaga cgtggaaagg caaaagcgca
    23281 ggatttttac tgctgggaat cacctgcggc atgggggtga tgaccaaagg ttttctcgcc
    23341 cttgccgtgc cggtattaag cgtgctgcca tgggtagcga cgcaaaaacg ctggaaagat
    23401 ctctttattt acggctggct ggcggttatc agttgcgtac tgacggttct cccctgggga
    23461 ctggcgatag cgcagcggga gcctgacttg tggcactatt ttttctgggt tgagcatatt
    23521 caacgctttg cactagatga tgcccaacat agagctccgt tctggtacta cgtgccggtc
    23581 atcattgccg gtagcctgcc gtggctggga ttactccccg gtgcactgta cacaggctgg
    23641 aaaaaccgca agcattccgc aaccgtctat ttgttgagct ggacgataat gccgctgctg
    23701 tttttctccg tcgctaaagg caaattgccc acctatattc tttcttgctt tgcacctctg
    23761 gcaatgctgt tggcgcatta cgctttgctg gcagcaaaaa ataatcctct ggcgctgcgg
    23821 attaatggct ggattaacat cgcttttggc gtcactggca ttattgccac atttgtgatc
    23881 tccccctggg gaccaatgaa cacgccggtg tggcaaacct tcgagagcta taaagtcttt
    23941 tgtgcctggt cgattttttc gctatgggca tttttcggct ggtacacctt aacaaacgtc
    24001 gaaaagacct ggtctttcgc cgcgctttgc ccgctggggc tggcgttgct ggtaggattt
    24061 tcaattcctg acagagtcat ggaaggaaaa catccgcaat tttttgtcga gatgacacaa
    24121 gaatcacttc agccaagccg ctatattctt actgatagcg tcggtgttgc cgcaggtctg
    24181 gcatggagcc tgcaacgcga tgacatcatc atgtatcgcc agacaggtga gttgaaatac
    24241 ggccttaatt atccggatgc gaaagggaga tttgtcagcg gtgatgagtt cgcaaactgg
    24301 cttaatcaac atcgtcagga ggggattatt acactcgtgc tttcggttga ccgcgatgaa
    24361 gatatcaaca gtctcgccat tccgcccgca gatgccatcg ttcgtcagga gcgtctggtg
    24421 ctgattcagt atcgtcccaa atgatctggc taacattagt ctttgccagc ttgcttagcg
    24481 ttgccgggca gttgtgtcag aaacaggcaa cctgctttgt ggcgataaac aaacggcgca
    24541 aacatatcgt gctgtggctg ggactggcgc tggcttgtct tggtcttgcc atggtgctct
    24601 ggctgctggt cttgcagaac gtaccggtag gcattgccta cccgatgtta agtctgaatt
    24661 ttgtctgggt gacgctggct gcagtaaaac tgtggcacga accggtatcg ccgcgtcact
    24721 ggtgtggggt ggcgttcatt attggtggca ttgtgatcct cgggagtacg gtgtaatggg
    24781 cctgatatgg ggattattca gcgtcattat tgcctcagtt gcgcaattaa gcctgggttt
    24841 tgcggcaagt catctgccgc cgatgacgca cttatgggat tttatcgcgg ctctgttagc
    24901 ttttggcctg gatgcccgga tactgttact cgggctgctg ggttatctgc tttccgtttt
    24961 ttgttggtat aaaacgctac ataaacttgc gctaagcaaa gcctatgcct tgctcagtat
    25021 gagttatgtg ctggtatgga ttgcttcaat ggtacttccc ggctgggaag ggactttttc
    25081 gctgaaagca ctactgggag tagcctgtat tatgagcggg ttgatgctga ttttcctgcc
    25141 cacgacaaaa caacgttact gagttttccc tgccgctgta cactgccgtt cccactcatc
    25201 ggggctataa ctgcttgccg agaggacttt caccgtatgc agcttttcac gattaataca
    25261 ataaagcgca ttctgcaaag gcgagagtaa atcccccact ttcacggcga agtcactctt
    25321 cacttcggcg atcattttta tcgcgccgcc agcatcgcag agcataagta catgtctgtt
    25381 atcttgttta ttgcagcacg attttttgtg ccagccattc cattgcatta tcctgtttgc
    25441 taagagtttt cacatcaatt tacagattaa cagataaact ctttatcaac ggcaatggaa
    25501 tggcagaaat gagaagtgaa acggagggtt aattattgct gacgttgcac ccactctttt
    25561 agcgcctgac gtgaaatttt aataccgccg tttttcagct ccggcggtaa agtcagccag
    25621 cgcaccggtt gttgaaaacg agccagctta tctttcaccc attcgccaag atcaacggtt
    25681 tgctggtcat actccaccac cgctaccggc cgatgaccaa actccttgtc ggcaacggaa
    25741 acgataaaca cctgcaaaac cgcaggatgt gcagcaataa cacgctcgac ttcttccggc
    25801 tgaataccct caccgccact gaagaacaga ttgtctaaac gtccgacaat ggtcagcttg
    25861 ccattatgca tctcaccgcg atcgcgcgta gcgtaccagc cttcgtcatt aaccagtgaa
    25921 accagttgcc cgttacgcca gtaaccttct gccatactgg cagcccgcag ccacacttca
    25981 ttattaacga ttttcacttc ccgacccggc agcggcaaac caacgtctgc caggccgtcg
    26041 gcttctttcg cacataccgt ggaggcaaac tcggtcagac catagccgca aaagcaacga
    26101 atcccctgct cgcgcgcctg ttccgtcaac tcgaccggga tagccgcgcc gccaagtaac
    26161 accgctttca gggaaacgga actgcggtta accagcaaac gccagagttg tgttggcacc
    26221 agtgaagcgt gagtacagcc tgccagcatt tgctccaatg gctgtttatc acgcaccgtc
    26281 atccgcgcac cagcgtataa ccagcgccac ataattccct gaccggagac gtgaaacagc
    26341 ggtaaagaga gcaaccagtc atcgtgatcg ccaaacggaa tcagcgataa cacaccttgc
    26401 gcactggcaa gatgggcctg ataagtatgg acagcggctt tcggcaaacc ggtagaaccc
    26461 gaggtcaacg tcattgagca cagacgcgtc ggctgccacg cagcggcatg tacgccttca
    26521 accagctgaa tgtgcagcga cgttaatgcc ggaaacgtgt tttccccatc cggcaccagc
    26581 gcaaattgca gcgtcagatt gggcagcaat tcttcaagca acggttgcgg cagctgaggg
    26641 ttcacgggca acacccgcgc cccgcattgc agtaacgcca gccaggcgag cagcgtttgc
    26701 ggcgtattcc acgcccgcaa catcacgccg ctgccctcaa ccacaccctg cgccgcaaag
    26761 cccgtagcta actcatcgac gcgagcacaa agctcgcgcc agttgagttg ctcgtcatta
    26821 agacgtaagg cgatggcttc tccccgcact tgccgccagt gacgccacgg ccagtcagag
    26881 aagatcataa caactgctcc agtacatcag cgtccactaa cggcagtggg ctccccggcc
    26941 agcgacgaac ctgctgcgcc tgcatcagat ccagcgtgtc cagtcccgga atggtgtctg
    27001 gcgttaacca ggcagcaatc cgcgccagtt gcgttagacc taagctcgac tcaatggaag
    27061 aactgatcac cgccgtcagc cccagcgcat gcgccgcctg tacctgctcg cgtacttttt
    27121 ccagactgcc cgtgagcgtg ggtttgataa ctaccgcgcg cacgccctct tcagccacaa
    27181 aggcaaaatc cggctcgcgc aggctttcat cccaggcaat ggcaatgccg gtttcacggg
    27241 caaacgctcg cgaatcatcg cgggttttgc acggctcttc gagaaacgcg atgcggtggc
    27301 gataatccgg gttaacatat ttggtaaact gctgaccttt cagcggtgtc caggcgcgat
    27361 ttgcgtcaag acgcaaatgc agatccggaa ttgcctccag caacagattc accaccatgc
    27421 cgtcgcgcac cgcttcgtac aatcccactt tgaccttcgc cactttctcg cccggcatat
    27481 cggcaagttt gaggatcaga tcgtccggat cgccattaca cagcggtgcc gcacggtagt
    27541 tggctgcttg cggcaacgta tctgtcagtt ctgccaatgc acagcttacg ccaaaggcca
    27601 cggaaggcat ctgcggtagc tcgcaatcgc ctgccagcca gttatttacc caggcaagca
    27661 gcacgctttg cgcctcttcc cagttttcct ggctgaagcc cggcagtggg gagatctccc
    27721 cccacccttc gcgctcgccc tcacgcaggc aaacgtacag cccgtcgcgg gtttttaacc
    27781 gcctgtcgcg cagaaccacc cccgcgtcca tggggatctg ccagcggtat acctgcgcgc
    27841 tacgcattac ggattccgtt taaatttgct gaaatcaggc tgacgtttct ggttgaaagc
    27901 gttgcgacct tcctgacctt cttccgtcat gtagaacagc atggtggcgt tgcccgccag
    27961 ctcctgcagc cccgcctgcc cgtcacagtc ggcgttcagc gcagctttca ggcagcgcag
    28021 cgccatcggg ctgttttgca gcatttcgcg gcaccaacgg acggtttctt tttccagatc
    28081 cgccagcggt accacggtgt tgacaaggcc catatccagc gcctgttttg cgtcgtactg
    28141 acggcacagg aaccagattt ctcgcgcttt tttctgcccg acgatacgag ccatgtagga
    28201 agcgccccag ccgccgtcga aggaaccgac tttcgggcca gtctgaccga agatggcatt
    28261 atctgctgcg atagtcaggt cgcacatcat gtgcagaacg tgaccaccgc cgatggagta
    28321 gccagccacc atcgcgacaa ccggtttcgg acaggtacgg atctgacgct ggaagtccag
    28381 cacgttcagg tgatgtacgc cggaatcatc tttatagccg ccgtaatcac cacgcacttt
    28441 ctggtcacca ccggagcaga acgctttatc gcctgcgcca gtcagaatga tcacgccgat
    28501 attgtcgtca taacgcgcat ctgccagtgc ctggatcatc tcttttaccg tcagaggacg
    28561 gaaggcattg cgcacctgcg gacgattaat ggtgattttt gcgataccgt cggtggattt
    28621 ttcataacaa atgtcctcga aaccttcgga gcagtcgtgc cattcaaccg gtgcgtaagg
    28681 cattgcttca tcaggataaa tcatattgtg tcctttagtc aaaaacgcaa gatctgcgcc
    28741 agacttgcga ttacgccagc gggattttcc cgatgcgcgt tatgtccggc gcgaggaatg
    28801 acatggcagt cggcagccag ttccgccgcc agggcgcgga atttgctgtc acgttcacca
    28861 cataaataat aaaacgcaaa tgtgcgggcg ctaaggttag cacgtaaatc aggctggacg
    28921 gcgagagaag tcgcctccag catggcggca agggttgcgc cattattgtt gctgcgcagc
    28981 gccaccagct cccggcgttg atcgtcattg agcgaggcaa aaacaggctg ttgataccag
    29041 tcggcaaata ccgccgttaa cggttctgtg cgaaaacgct gcgcccattg gcgatcggaa
    29101 cgctgacgtt ccgcacgttg ttcagcattt tgcagccccg gatgcccgcc ttcgataata
    29161 accccacaaa gccccgccaa cccctggcaa gccgccatca tcgccacccg tccgccaagc
    29221 gagtacccca ccagccagaa gtcaaggatg ttgtaactaa ccaaggtttt acgcagtaag
    29281 tcggtgacat catcaaatcc atcgacgcta atcgccgccg aaccaccatg acctgggaga
    29341 tcaacataca accgtgagta gtcggcaaac gcctcgccca cttcttgcca ttcgtggcaa
    29401 tcgccggaaa aaccgtgcaa aaacaccagc cagggtaaac ctggttttcc gtgttttgcc
    29461 tgcgcgtgca ggatcataaa tggcttacct gcgccagaag ttgctggagc gtttgcgcac
    29521 catcggtgtc gttaaccacc atttcaatca ccgtggtggt tggcgtgcgc caggcgtcgg
    29581 caaatgccgt ttcaagttcc tgccagtttt gcggacgatg atatttcagc tcgaacatcg
    29641 cggcggcgtg ctcaaaatgg acgttttgcg gcatcagata gaaacgctca cgctcgcttt
    29701 gcggcgttgg caacagcgag aaaatttgcc cgccgttgtt gttcaccaca attaatacca
    29761 gcggcgcaga aacctgacgc aataacgcca gcgcgttgag atcgtaaagt gcggagagat
    29821 cgcccacaat cgccagcgtc ggtttgccgc ttgcccgctg aacgcctgcg gcggtcgaaa
    29881 gcagtccgtc gataccgctg gcccctcggt tgctgtacac cgggtaacct gccggaagtt
    29941 gcgaaagcgc atcaatcaga cgtaccacca ggctgttacc aacaaacaat tgcccctgtt
    30001 caggcagata gtcgctgatg cgatgcgcca gttgcgcttc gccaaacgca tcacggcggg
    30061 caataaccgc ctgcattgcc tgttccgcca ggcgcgggat ttcaacgcac cagggctggc
    30121 gtttttctgc cggatgcagc tccagccagt cggcaatatt gccaattaag cgacgtccgc
    30181 gatggtgtgc cggatcaagt cgcccttcaa tgtcatcaac aatccagtac tcttctggtt
    30241 cacagcttgc ctgccattgc aggagccgtt tccccgtcag gctgcttccc agttgcacca
    30301 caatttgtgc ctgctgcagc tcgctggtcg ctttggcatt gcctaaccaa agatcggcac
    30361 acggcagcgg ctgcccggtt tgtgacagca catcgccaat cagcggccag ccaagagttt
    30421 gcgcccacag ggcaactttt ttgccctctt ccgcactcat gcgcccggca accaccacgc
    30481 cgcgcttttg tcgccagaag aaccagtcgc gctgtttttc actttccaga cgaggcgctt
    30541 cacgcagcca cggtttgtcg tcctgccacc agtcacccag acgctgttgc cagctaagcc
    30601 cggtatcgtc catttcgcca tacagcggtt cagcaaacgg gcagttgata tggactcccc
    30661 ccgcatgaag cgtaccgaga gcgtggtcga tggtagaaac cagccaacgt gcggggatat
    30721 cctgggtcgg gcgcggcaat gaaatactgt gcgtggggtg agaggcgaac attcccggct
    30781 ggcgaattgc ctgattcgcg ccgcagtcaa ttagctccgg cgggcgatcg gcggttaaga
    30841 gaatcagttt ttctccggtt aacccggctt caatcagtgc cggatagaga tttgccaccg
    30901 ccgtgccgga ggtcacaatc accgccaccg gctgcttgct gactttcgcc agccccagcg
    30961 ccagatgccc caacccacgc tcatcgaaat gggtgtggtg aatgaatgcg gaattctccg
    31021 ccgccgctaa cgttaacggt gtagaacgcg agcctggggc gatacagatg tgtctgacgc
    31081 cgtgacgcgt taatgcttcc agaatgaccg ccgcccagcg tcggttaaat gcgcttactg
    31141 acatgagttt gtccggtatc aatattgcgg ctaagtataa ggagctacaa aaatagatta
    31201 ttgatatgaa tcggtaatga tgcgactcat tactattcca tttgtaataa agtacgcagc
    31261 cctgccgctt tgttgtcgat ttcctgccac tcttgctcgg ggtcggaacc acggacaatg
    31321 cccgcgccag catataatcg cacgacattg ccgctaattt ttgctgagcg cagggaaacg
    31381 cagaattcgc tttgttgtaa tgagagatag cccgcagaac cggcgtacca ttctcgggtg
    31441 aacggttcgt gacgggcgat aaactgtcgc gccagatcgc gcggtaagcc agcaactgct
    31501 gccgttggct gcaactgatg taaacagatc acatcatccg ctttgttgag tgaagtccag
    31561 atacagcggc gaagatgctg cactttacgc agacgcagta cctgcggcgg taaaacatcc
    31621 agcgtctggg tatcggcctg taatcgttgg cagatatctt ccaccaccag catgttctcg
    31681 cgctggtttt tatcatccgc catcagcctc tctcctaact gctgcgcctg cttatcatca
    31741 ggatgatttg ctactgttcc cgccagcgct tcagtacgca gcgctttgtc acgccgccgc
    31801 cataaccgtt ccggtgaaga gccaagaaaa gcattttcgc catcaaaggc catgtaaaaa
    31861 tggtagcaat tcagattcag tcgacgactg gcagccatca tcgccgccgc gttgaccgga
    31921 cttgcgaaat gcaggtcagt tgcccgagcg agcaccactt tgtcgagctc acctttggcg
    31981 atggttttcg ttgccagttc gattaattgc gtccagcccg ttttgtccgg ccagtgttgt
    32041 tctcgcgtgg tggttaaatg taacccaggc aagggcttga tactcaccag tgtggcgata
    32101 aattcttttg cctgaatcgc atcgtgctga agggagcttt cgctgaatag cgtcaaccgc
    32161 agcgtggctt taccgccaca gcgtcgccat tccaggcggg gtaaaagtaa attgccctgc
    32221 gacgggtcaa aagcattcag cccccaaatg cgtaagtcgg cgtgttccgg gtgctggcga
    32281 agaaaacgtt gtgcctggtc caacgacgta aaacgggtaa tcgcgcccag gacggcagct
    32341 tcttcatcac cattacgttg ttgccagtag aattgcgggt acgtttgctg actggccagc
    32401 cagctcaagg catcaaaagc gtctttgaga gggaaaggaa tatcgataac ccgaatgccg
    32461 ggtgtcgccg gaatctcttg cgacaaatgg cgcagtagat tttccagcgc cgtagtaagt
    32521 gattgcacgc ggacctctcc ctgttaaaag cctcacatta tacggggtac tacaaaaaaa
    32581 atgcagtacc ccggtgtagg gaggttttaa cggcgtgcca gcaacagtcc tagtaccagc
    32641 ccgacggccg cgcccacacc aattccttgc cagggttttt cgtggacgta gtcatcagca
    32701 cgataaacag cctgcttcgc ccgatagtaa taactgtctg aagcctggct aacccgtttt
    32761 ttcacatcat ccagcgcttt ttctgcacgc gctttcagct caacatattt ctgatcggcg
    32821 ggctcgccag aggagcggag cacctcttcc agtgtttcac taagcagcgt caggtcgtca
    32881 tcgatacgtg tatcaccaaa ctgattagac attctcgttc tccatttgcg taaaacctgt
    32941 cactaactat agacaagagt tgttgattac gcctgaatta cctcgcgtgc catcccaatg
    33001 tgtgggatac aatcttcttc atagacctcc gtcacaggga taaaaccgaa actctggtag
    33061 aagttttgca gatgcgcctg cgccccgagg taaacaggct tatcaggcca gtgatgcgta
    33121 catgtttcga gtgttttact catcagctgc tgccccactt tttcaccacg caacgcttca
    33181 ctgacaatca cccgacctat aacgaccggc tcaagatcat catcactttt cagaatcctc
    33241 gcatacgcca ccagttcatc atttttccaa ccgagaatat ggcgattatc ccccgtcaaa
    33301 tcatcaccat cgatatcctg ataagggcaa ttctgctcga caacaaacac cgcgcaacgc
    33361 agttgtaata aggcataaag ctgggaaacc gataattcag agtgatgcag atcttgccat
    33421 tcaatcataa tgctctcctt ttagatgtat gccttatact agccggaata ttttttgaaa
    33481 cgtgatgaac tcatggaatt aattttttta ggtacttcag ccggtgtgcc aacccgcacg
    33541 cgcaatgtca cggcaatatt gcttaacctg caacatccga cccagagcgg actttggttg
    33601 tttgactgcg gtgaaggcac ccagcatcag ctactgcata ccgcctttaa ccctggaaaa
    33661 ctggacaaga tttttatcag tcaccttcat ggcgatcatc tttttggttt acccggcttg
    33721 ctgtgcagtc gttctatgtc aggcattatc caacccttaa cgatttatgg tccgcaaggt
    33781 atccgtgaat ttgtggaaac cgcgctgcgg attagcggct catggaccga ttatccgctg
    33841 gaaattgtcg aaattggcgc tggcgaaatt ctcgatgatg gcctgcgcaa agtaaccgct
    33901 tatccgctgg aacacccact ggaatgttat ggctatcgta ttgaagaaca tgataaaccg
    33961 ggtgcattaa atgcccaggc attaaaagct gctggcgtgc cgcctggccc actgtttcag
    34021 gaattaaaag cgggcaaaac aatcacgctg gaagatggaa ggcagattaa cggcgcagat
    34081 tacttagctg ctccagtgcc aggtaaagcg ctcgctattt tcggcgatac cggcccctgc
    34141 gatgccgcac ttgacctggc taaaggtgtc gatgtcatgg tgcacgaagc gacgctggat
    34201 ataaccatgg aagccaaagc caatagtcgc ggccatagct ctacacgcca ggctgcgaca
    34261 ctagcccgtg aggctggagt cggcaagcta atcattaccc acgtcagctc gcgctatgat
    34321 gacaaaggtt gtcaacacct gttacgtgaa tgcaggtcaa ttttcccggc gactgaactg
    34381 gcgaatgatt tcaccgtgtt taacgtttaa ctctgcttat tattaaaaac agggtgaaac
    34441 ttgccctgtt atagcaaccc gcgctacata tatccaccca cacaggcata ttgagcgatt
    34501 tttgatattt attcatctcc ttacgcacta tattcagagc aataagttgc ctcttttcat
    34561 cttgattcag cacaaccttt gcaactaaaa ggagcccatg atggttacag ttgtcagcaa
    34621 ttattgtcaa ttatctcaaa cccaactcag tcagacattt gcagaaaaat ttactgtgac
    34681 cgaggaatta ctgcagtctt taaaaaaaac agcgttatcc ggagatgaag aaagtattga
    34741 gttactgcat aatattgcgt taggttatga tgaatttgga aaaaaagcag aagatattct
    34801 ttaccatatt gttagaaacc caacaaatga gacctatcga ttatccgact tattaaaaat
    34861 gcctgtttaa aattatataa tttggcacat accgcaacca actcccacct taaaccaact
    34921 ggtccggata attcagatgt tctcctgttc aaaaagctat tctccccttc gaaattaatg
    34981 acaattatcg gtaatgaaat tcctcttata tctgaaaaac agtcgctttc aaaggtgctt
    35041 ttaaatgatg agaataatga actgagtgat ggtacaaact tctgggataa aaatcgtcaa
    35101 ttaaccacag atgaaatagc ttgctatctt cagaagatcg ccgctaatgc aaaaaatact
    35161 caagtcaatt atcctactgg tctctacgtc ccctactcca ccagaactca cctggaagac
    35221 gctctcaatg aaaatattaa gagcgatcca tcatggccga atgaagtcca gt
//
LOCUS       DAETPZ010000036        35253 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.036, whole genome shotgun sequence.
ACCESSION   DAETPZ010000036 DAETPZ010000000
VERSION     DAETPZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.036"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..1152)
                     /gene="setB"
                     /locus_tag="K0W59_001981"
     CDS             complement(<1..1152)
                     /gene="setB"
                     /locus_tag="K0W59_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HBI3023021.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATL"
     gene            1520..2650
                     /gene="fruB"
                     /locus_tag="K0W59_001982"
     CDS             1520..2650
                     /gene="fruB"
                     /locus_tag="K0W59_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HBI3023022.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2650..3588
                     /gene="fruK"
                     /locus_tag="K0W59_001983"
     CDS             2650..3588
                     /gene="fruK"
                     /locus_tag="K0W59_001983"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBI3023023.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3605..5296
                     /gene="fruA"
                     /locus_tag="K0W59_001984"
     CDS             3605..5296
                     /gene="fruA"
                     /locus_tag="K0W59_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HBI3023024.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5720..6661
                     /locus_tag="K0W59_001985"
     CDS             5720..6661
                     /locus_tag="K0W59_001985"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HBI3023025.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6649..7587
                     /gene="psuG"
                     /locus_tag="K0W59_001986"
     CDS             6649..7587
                     /gene="psuG"
                     /locus_tag="K0W59_001986"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HBI3023026.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7681..8931
                     /locus_tag="K0W59_001987"
     CDS             7681..8931
                     /locus_tag="K0W59_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBI3023027.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFIA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9003..9701)
                     /gene="yeiL"
                     /locus_tag="K0W59_001988"
     CDS             complement(9003..9701)
                     /gene="yeiL"
                     /locus_tag="K0W59_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HBI3023028.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLMNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLT
                     LEMDPENKFSGMMQ"
     gene            9832..10773
                     /gene="rihB"
                     /locus_tag="K0W59_001989"
     CDS             9832..10773
                     /gene="rihB"
                     /locus_tag="K0W59_001989"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HBI3023029.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10873..12123
                     /locus_tag="K0W59_001990"
     CDS             10873..12123
                     /locus_tag="K0W59_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBI3023030.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVIGILIRILGGIFQKALNISKIESFVAVTTIFLVQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12179..13263)
                     /locus_tag="K0W59_001991"
                     /pseudo
     CDS             complement(12179..13263)
                     /locus_tag="K0W59_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            complement(13266..14123)
                     /gene="nfo"
                     /locus_tag="K0W59_001992"
     CDS             complement(13266..14123)
                     /gene="nfo"
                     /locus_tag="K0W59_001992"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBI3023031.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14197..15246)
                     /locus_tag="K0W59_001993"
     CDS             complement(14197..15246)
                     /locus_tag="K0W59_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HBI3023032.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15345..16226
                     /locus_tag="K0W59_001994"
     CDS             15345..16226
                     /locus_tag="K0W59_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3023033.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16432..17901
                     /gene="lysP"
                     /locus_tag="K0W59_001995"
     CDS             16432..17901
                     /gene="lysP"
                     /locus_tag="K0W59_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HBI3023034.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVLAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18193..20184
                     /gene="cirA"
                     /locus_tag="K0W59_001996"
     CDS             18193..20184
                     /gene="cirA"
                     /locus_tag="K0W59_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HBI3023035.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YCVSHNGRWDYGTSELKSYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGGESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYSVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(20216..21052)
                     /gene="yeiG"
                     /locus_tag="K0W59_001997"
     CDS             complement(20216..21052)
                     /gene="yeiG"
                     /locus_tag="K0W59_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HBI3023036.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATYYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            21310..21978
                     /gene="folE"
                     /locus_tag="K0W59_001998"
     CDS             21310..21978
                     /gene="folE"
                     /locus_tag="K0W59_001998"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBI3023037.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21995..23152
                     /locus_tag="K0W59_001999"
     CDS             21995..23152
                     /locus_tag="K0W59_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HBI3023038.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWWLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGLAISKTSR"
     gene            23294..24333
                     /gene="galS"
                     /locus_tag="K0W59_002000"
                     /pseudo
     CDS             23294..24333
                     /gene="galS"
                     /locus_tag="K0W59_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
     gene            24613..25611
                     /gene="mglB"
                     /locus_tag="K0W59_002001"
     CDS             24613..25611
                     /gene="mglB"
                     /locus_tag="K0W59_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HBI3023039.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25672..27192
                     /gene="mglA"
                     /locus_tag="K0W59_002002"
     CDS             25672..27192
                     /gene="mglA"
                     /locus_tag="K0W59_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HBI3023040.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIGMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27208..28218
                     /gene="mglC"
                     /locus_tag="K0W59_002003"
     CDS             27208..28218
                     /gene="mglC"
                     /locus_tag="K0W59_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HBI3023041.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28254..29489)
                     /gene="preA"
                     /locus_tag="K0W59_002004"
     CDS             complement(28254..29489)
                     /gene="preA"
                     /locus_tag="K0W59_002004"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HBI3023042.1"
                     /translation="MLTKDLSITFCGVNFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLTALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVKKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(29483..30722)
                     /gene="preT"
                     /locus_tag="K0W59_002005"
                     /pseudo
     CDS             complement(29483..30722)
                     /gene="preT"
                     /locus_tag="K0W59_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            complement(30916..31155)
                     /locus_tag="K0W59_002006"
     CDS             complement(30916..31155)
                     /locus_tag="K0W59_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HBI3023043.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31158..31877)
                     /gene="sanA"
                     /locus_tag="K0W59_002007"
     CDS             complement(31158..31877)
                     /gene="sanA"
                     /locus_tag="K0W59_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HBI3023044.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(32027..32911)
                     /gene="cdd"
                     /locus_tag="K0W59_002008"
     CDS             complement(32027..32911)
                     /gene="cdd"
                     /locus_tag="K0W59_002008"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBI3023045.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(33041..33736)
                     /locus_tag="K0W59_002009"
     CDS             complement(33041..33736)
                     /locus_tag="K0W59_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HBI3023046.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEITASILPKSVTTPIAMSVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33733..34131)
                     /locus_tag="K0W59_002010"
     CDS             complement(33733..34131)
                     /locus_tag="K0W59_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBI3023047.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34370..>35253
                     /gene="dusC"
                     /locus_tag="K0W59_002011"
     CDS             34370..>35253
                     /gene="dusC"
                     /locus_tag="K0W59_002011"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HBI3023048.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVR"
BASE COUNT         8662 a         8615 c         9306 g         8670 t
ORIGIN      
        1 cagagtggcg ataatcatca ccatcgcaaa ccagaacaca gcgtgataat tccagatctc
       61 ggcgacgatg cccgccactg atcctgcgat gatccagccc acgcgcgacg tgttggtata
      121 gagcgtggtg gctgaacctg cctgaccggg catcaaatcc tgaaaataga gcatcccgat
      181 gccgcccaga atgccaataa aaatagcatt tagcagctgc aagcccaaca gaatggcagg
      241 tgaatgcgcc atcagcattc ctgcgtaaaa acagacgcca cccacggcag caacgcgcat
      301 taagaaacgc ttacccagac gtttggcgaa atatccggca atcaacatcg tcgggatttc
      361 cagcccggcg gcggtcccca tcatcacacc ggccagtttc tcgggaagat gcagttcgtt
      421 gataataaat agcggcatgt tgatgatgta caggctgttc gagccccaca tcaatgtaca
      481 aatgacaaac agcagtagcg tatcgcgacg gttacggcgc ggcgcttcaa ccgtgccggt
      541 cgccagcgga agctcttttt gcatcgacgg taaaaacagc cacaccatca cgccgcaaac
      601 aataaacgct accgctgcgc tcagatacat taccgtaaag ctgaaaccca tcgctaacgc
      661 ataagccagc ggtgggccaa tgacccatgc cagtgaaacc tgagcgcgta aaaaagagct
      721 gaacatcacc gcctcacgtc cggttttgtc ggcatgttca cgggcaaggg caaacatttg
      781 cgggttagcg gtcgagccaa agctgctaag aaagacgcca acgaatagca aaacaaagta
      841 gttgcgattc caggcaaaaa gggtgcaggc cagcacgcct aacaggcagc aaaagacaat
      901 cagcgatttg cgatcgccgc gcttatcaga gcgcccagcg agaaactgac ttaccagaat
      961 cccaatgaca gcgctgccgg taaagaagaa tcccaccatc gccggacggg catgtacttc
     1021 atcggtaagg aaaatactga gtgtcggggt ttgcagagca cccgcaatac cggtgagaaa
     1081 ggcaacgatt aaaaacgccg tcgaggtcag gtcaaacgat ttcgcgctgg agactgcggg
     1141 ggagttatgc atgctggtcg atcactggaa gaggaagacg cggagtttac gctgcttgtc
     1201 agaaaataaa cagacagcga aatcaaaagc gccacgaata atcaaaatgc catttcactg
     1261 ttgctgattg gctgctgacg ctgctgaaat gaaattgctg ataacggatt ttcccatcag
     1321 caattaggaa aaatgacaaa aaattgtgca gcacatcaaa cttttgctca taactttacg
     1381 gctttccttg cgtgctgaaa cagaagtatt atgctttctt gaaacgtttc agcgcgatct
     1441 tgtctttaac cctaagccag gttggcgctt tttttctcat agaggctgaa tcgtttcaat
     1501 tcagcaagag aggagaacta tgttccagtt atccgtacag gacatccatc cgggcgaaaa
     1561 ggccggagac aaagaagagg cgattcgcca ggtcgctgcg gcgctggtgc aggccggtaa
     1621 tgtagcagaa ggctacgtca atggcatgct ggcgcgcgaa cagcaaacct caacgttcct
     1681 cggcaatggt attgctattc cacacggcac taccgacacc cgcgatcagg tgctgaaaac
     1741 cggcgttcag gtatttcagt tcccggaagg cgtcacctgg ggtgacggtc aggtagcgta
     1801 cgtggcaatc ggtattgctg ccagctcgga taaacatctg ggcctgctac gccagctgac
     1861 ccacgtactg agcgatgatt ccgttgctga acaactgaag tcagcaacaa cagcagaaga
     1921 acttcgcgca ttactgatgg gcgaaaagca gagtgagcag ctgaagctcg acaacgaaat
     1981 gctgacgctg gatatcgtcg ccagcgatct gctgactctt caggcgctga acgctgcgcg
     2041 tctgaaagag gcgagggcag ttgacgccac tttcgtcacc aaagccatca atgaacaacc
     2101 gctgaatctc ggacagggta tctggctgag cgatagcgcc gaaggcaatc tgcgtagtgc
     2161 gattgcggta agccgtgcgg caaatgcttt tgatgtggac ggcgaaacgg cagccatgct
     2221 ggtgagtgtg gcgatgaatg acgatcagcc catcgcggtt cttaagcgtc tcgctgattt
     2281 gttgctcgac aataaagctg accgcttgct gaaagcggat gcggcaacgt tgctggcgct
     2341 gctgaccagc gatgatgcgc cgaccgacga cgtgttaagc gcggagtttg tggtgcgcaa
     2401 tgaacacggc ctgcatgctc gtccaggtac catgctggtc aataccatta aacaatttaa
     2461 cagtgatatt accgtgacaa accttgatgg caccggcaaa ccggcaaacg gacgtagtct
     2521 gatgaaagtt gtggcacttg gcgttaagaa aggtcatcgc ctacgcttta ccgcccaggg
     2581 tgcagatgct gaacaggcgc tgaaagccat tggcgacgct atcgctgctg gtcttgggga
     2641 gggcgcataa tgagcagacg tgttgctacg atcaccctta atccggctta tgaccttgtt
     2701 ggtttctgcc cggaaattga acgcggcgaa gtgaacctgg tgaaaaccac cggtctgcat
     2761 gcggcgggta aaggcatcaa cgtggccaaa gtattaaaag acctgggaat tgatgtcacc
     2821 gttggcggct tcctgggtaa agacaatcag gatggttttc agcaactgtt cagcgagctg
     2881 ggcattgcca accgtttcca ggttgtacag gggcgcaccc gaattaacgt taagctgacg
     2941 gaaaaagacg gcgaagtgac cgacttcaac ttctcgggtt ttgaagtcac ccccgccgac
     3001 tgggaacgct ttgtgactga ttctctgagc tggctcggtc agttcgatat ggtctgtgtc
     3061 agcggaagct taccgtcagg cgtcagcccg gaagcgttca ccgactggat gactcgcctg
     3121 cgtagtcagt gtccttgcat tatctttgat agtagccgtg aagcgttagt agcaggtttg
     3181 aaagcggcac cgtggctggt gaaacctaac cgccgcgagt tggaaatctg ggcaggccgt
     3241 aaactgcctg aaatgaaaga tgtgattgaa gctgcacatg cgctacgtga acaaggtatt
     3301 gcgcatgttg ttatttcact gggtgccgaa ggcgcgcttt gggttaatgc ctccggcgaa
     3361 tggatcgcta aaccaccgtc agtcgatgtc gtaagcaccg ttggcgcagg ggattctatg
     3421 gttggtggcc tgatttatgg cttgctgatg cgtgaatcca gtgaacacac actgcgtctg
     3481 gcgacagctg ttgcagccct ggcggtaagt caaagcaatg tgggtattac cgatcgtccg
     3541 cagttggccg caatgatggc gcgcgtcgac ttacaacctt ttaactgaca gcaggagagg
     3601 cataatgaaa acgctgctga ttattgacgc taatctcggt caggcacgcg cctatatggc
     3661 gaagaccctg ctgggcgcgg cggcgcgaaa agcaaaactg gaaatcatcg acaatccgaa
     3721 cgacgcagaa atggcgattg ttctcggtga ttccatcccg aatgacagcg cgctgaacgg
     3781 taaaaatgtc tggctgggtg atatttcccg ggcagttgcg caccctgagc tgttcctgag
     3841 tgaagccaaa ggccatgcga aaccttacac tgcgccggtc actgcgacag caccggttgc
     3901 cgccagcggt ccgaaacgcg tagttgcggt gactgcttgc ccgactggcg tagcacacac
     3961 ctttatggcg gctgaagcca ttgaaaccga agcgaaaaaa cgtggctggt gggtgaaagt
     4021 tgaaacccgt ggttctgttg gcgcgggtaa tgcaatcact cccgaggaag tagccgcagc
     4081 ggatctggtg attgtggcgg cagatatcga agtggatctg gcgaaatttg ctggtaaacc
     4141 gatgtatcgt acctctaccg gtctggcgct gaagaaaacc tcgcaggaac tggataaagc
     4201 ggttgctgaa gcaacgccgt atgaaccggc gggcaaagct caaacggcga ccactgaagg
     4261 taagaaagag agtgcaggcg cttatcgtca cttgctaacg ggcgtctctt acatgctgcc
     4321 gatggtcgtt gctggtggtc tgtgtatcgc gctttctttt gcttttggta tcgaagcgtt
     4381 taaagagccg ggtacgttgg cagcggcgct gatgcagatt ggtggtggtt cagcctttgc
     4441 gctgatggtg ccggtactgg caggttatat tgccttctcc attgccgatc gtccgggcct
     4501 cacgccgggt ctgattggcg gtatgctggc ggtcagcacc ggttctggct tcattggcgg
     4561 tattattgcg ggcttcctgg ctggttacat tgcgaagtta atcagtacgc aactgaaact
     4621 gccgcagagt atggaggcgc tgaaaccgat cctgatcatt ccgctaattt ccagtctggt
     4681 ggtcggtctg gcgatgatct acctgatcgg taaaccagtt gctggcattc tcgaagggct
     4741 gactcactgg ctgcagacca tggggactgc gaatgcggtt ctgctggggg cgatcctcgg
     4801 tggcatgatg tgtactgaca tgggcggtcc ggtaaacaaa gcagcgtacg cattcggtgt
     4861 gggtctgctg agtactcaaa cctatggccc gatggcggcg attatggcgg caggtatggt
     4921 gccaccgctg gcaatgggcc tggcaacaat ggtggcgcgt cgcaaattcg acaaagcgca
     4981 gcaggaaggt ggcaaagccg ctctggtatt ggggctgtgc ttcatttcgg aaggtgcaat
     5041 tccgtttgct gctcgtgatc cgatgcgtgt gctgccgtgc tgtatcgtgg gtggggcgct
     5101 gactggcgca atctcaatgg cgattggtgc gaaactgatg gcaccgcacg gtggtctgtt
     5161 tgttctgctg atccctggcg ctattacgcc ggtattgggt tacctggtag caattattgc
     5221 cggtacgctg gtggcgggtt tggcctatgc cttcctgaaa cgtccggaag tggacgcagt
     5281 agcgaaagca gcgtaataaa aggtgtgtta cagggcagaa atttcctgcc ctgtaacatt
     5341 tgatgagcgc aaagagaatg accagcttgt gaatgtaatt ttatgaattt aaatggtttt
     5401 atctttcagt agaaacttca aacgttcatc aaacactaag gttatttcct caagggcaga
     5461 ttattttctg ccctttttta gtgttcctgt tctgtgtatc ccgtcacaaa ttcctcgctt
     5521 ttgtaattca ggcatttaat cgcattttgt gagcctgctc aaatctgccc attttgcctc
     5581 tctggcggta atttacttta cgtaaaacat gcatactatg agcacatgtt taaatatgaa
     5641 cacatgttta aaccgtcttt gcagtatcta tattcttgtg gattgacagg gttggtaatt
     5701 ttttaccaga ggacgtgcta tgcgcgaaaa ggattatgtc gtaattatag gttcggcgaa
     5761 tattgatgtc gccggatatt cacatgaatc attaaattat gcggattcaa atccaggtaa
     5821 aataaaattt acgcctggtg gagtagggcg caatattgca caaaacctgg cgttgctggg
     5881 taacaaagcc tggctactga gcgccgtagg cagtgatttt tatggtcaat cgctgctaac
     5941 gcaaaccaat caatctggtg tttatgtcga taaatgcctg attgtgccgg gagaaaatac
     6001 gtcgagttat ttatcattac tcgataatac cggtgaaatg ctggttgcta taaatgacat
     6061 gaatattagc aacgctatta cagctgaata tctcgcacag caccgtgaat ttattcagag
     6121 ggcaaaggtc attgtcgcgg actgtaatat cagtgaagag gcactggcat ggattctgga
     6181 taatgccgcc aacgtacccg tatttgtcga tccggtttca gcgtggaaat gtgtcaaagt
     6241 gcgcgaccgt ctaaatcaga tccacactct gaagccaaac cgccttgaag cggaaaccct
     6301 gagtgggatt gcgctgtcag ggcgtgacga tgtggcaaaa gttgctgcct ggttccatca
     6361 acatggcctg aaccgactgg tattgagcat gggcggcgac ggcgtttatt acagcgatat
     6421 cagcggtgaa aatggctggt ctgcgccgat caaaaccaat gttattaatg ttaccggagc
     6481 gggcgatgcc atgatggcgg gacttgcttc gtgttgggta gacggaatgc cgtttgccga
     6541 atctgttcgt ttcgcacagg gatgttcgtc aatggcgctc tcctgtgaat acaccaataa
     6601 ccccgattta tcgattgcca acgttatatc gttagtggag aacgcagaat gtctgaatta
     6661 aaaatttccc ctgaattatt acaaatttct ccggaagtgc aggacgcttt aaaaaacaaa
     6721 aaaccggttg tggcgctgga atcaaccatt atttctcacg ggatgccgtt cccacaaaat
     6781 gcccagaccg caattgaagt agaagaaact attcgtaaac agggcgcagt acctgccact
     6841 atcgccatta ttggcggcgt gatgaaagtg ggtttaagca aagaaaaaat tgaattactg
     6901 ggtcgtgaag ggcataacgt gaccaaagtt agtcgtcgcg atttaccttt tgttgttgcc
     6961 gccggaaaaa atggcgcaac cactgtggct tcaacgatga ttattgcggc gcttgccgga
     7021 attaaagtat ttgccaccgg gggaattggt ggtgtgcatc gcggggcgga acataccttc
     7081 gatatttctg ccgatttgca agaactggca aatactaatg tcaccgttgt ttgtgccggg
     7141 gcgaaatcta ttctcgattt aggattaacc actgagtatt tagaaacctt cggtgtgccg
     7201 ttaattggtt atcagactaa agcgctgcct gcgtttttct gccgtaccag ctcgtttgac
     7261 gtcagcattc gtctcgacag cgccagtgaa attgcccgtg caatggcggt gaaatggcaa
     7321 agcgggctga acggtggcct cgtggtagcg aacccgatcc cggaacagtt tgcgatgccg
     7381 gaggaatcta tcaatgcagc catagatcaa gccgtcgccg aagccgaaga gcagggcgtt
     7441 attggtaaag aaagtacacc gttcctgctg gcgcgcgttg ctgagctgac cggcggtgac
     7501 agcctgaaat ccaatatcca gctggtgttc aacaacgcca ttctggcgag tgaaattgcc
     7561 aaagaatacc agcgtctcgc gggttaattt ctgacgggca gaaagccgtc gcccgtactc
     7621 atcaagcata ccaaaacctg gcgctgacgc cgggtttact ggcatgaagg gaaaatcatt
     7681 atggatataa tgagaagtgt tgtggggatg gtggtgttac tggcaatagc atttctgttg
     7741 tcagtgaata aaaagagcat cagtttgcgc acggttggag ccgcactgct gctgcaaatc
     7801 gctattggtg gcatcatgct ctacttccca ccgggaaaat gggcagtaga acaggcggca
     7861 ttaggcgttc ataaagtgat gtcttacagt gatgccggta gcgccttcat ttttggttcg
     7921 ctggttgggc cgaaaatgga tgtcctgttt gacggtgcgg gttttatctt cgcctttcgc
     7981 gtacttccgg cgattatttt cgttactgcg ctcatcagtc tgctgtacta cattggcgtg
     8041 atggggctgc tgattcgcat ccttggcagc attttccaga aagccctcaa catcagcaaa
     8101 atcgaatctt ttgttgcggt taccactatt ttcctcgggc aaaatgagat cccggcgatc
     8161 gttaaaccgt ttatcgatcg cgtgaatcgc aacgagttgt ttaccgcaat ttgtagcggg
     8221 atggcgtcca ttgctggttc gatgatgatt ggttatgccg gaatgggcgt accaattgac
     8281 tacctgttag cggcatcgct gatggcgatc cccggcggta ttttgtttgc acgtattctt
     8341 agcccggcaa ccgagccttc gcaggtcaca tttgaaaatc tgtcgttcag cgaaacgccg
     8401 ccaaaaagct ttatcgaagc ggcggcgagc ggtgcgatga ccgggctgaa aatcgccgct
     8461 ggtgtagcga cggtggtaat ggcgtttgtc gcaattattg cgctgatcaa cggcattatc
     8521 ggcggaattg gcggctggtt tggtttcgcc aatgcctctc tggaaagtat ttttggctat
     8581 gtgctggcac cgctggcgtg gatcatgggt gtggactgga gtgatgccaa ccttgctggt
     8641 agcctgattg ggcagaaact ggcgattaac gaattcatcg cttacctgag tttctcccca
     8701 tacctgcaaa cgggcggcac gctggaagtg aaaaccgtgg cgattatatc ctttgcgctt
     8761 tgtggttttg ctaactttgg ttctatcggt gttgtcgttg gcgcattttc ggctatttcg
     8821 ccaaaacgtg ctccagaaat cgcccagctt ggtttacggg cgctggcagc agcaacgctt
     8881 tccaacctga tgagtgcgac tattgccggg ttctttattg gtctggcgta atgctacgat
     8941 ttgtagaccg gataaaacgt attaagcgtt gcatccggca atcgcgctat tgcaaataat
     9001 ttttactgca tcatcccgga gaatttattc tccgggtcca tctccagcgt cagtcctgac
     9061 aactgctttc tgtttttaat tagatatcct ttcttgcttt taattaataa accgtcgtga
     9121 atgaattgcg cgagaacata taaaagatgt cgataagaaa cgcctaaata ctctgccgct
     9181 tgcgtatgct tttcgtgata aagatcacct tcttgtgaga gtaatataaa tgctgccagg
     9241 cgattcatta acggaaatga ttgattctga gttaaagaaa caatattacg ataattttta
     9301 tgacttaagg tgacgcaaag ttttcgtaaa aatagggtgt cgtttaataa cagcggacgg
     9361 taatgtttca tagggagcgc aaggcaccaa cactcttcaa tagcctgcac cgcacgcggt
     9421 tcatggtctt tgtcgattaa ctcaatctcg ccaataaaac agggagcagc aaagaaatcg
     9481 atcagcgaca cgcgaccatt agcaagcgtg gcgtaaagcc tggcgcggcc tcgcgtcaga
     9541 taaaacagcc atgacggctg ttgcccttcc tgcacaatgt agtcacgcgc taaaaaatga
     9601 aacagccgcg tatcggctga aacatccgtt gaaaagcaat ccttaaacgc agattcactc
     9661 atcaattgct gcttaaggtc attattgtgg atttctttca cgatgtttat ccgctatgag
     9721 atttctcata tttagcatac gtacgctgtt gttaagattc aatcatcagc ccggtcgaat
     9781 tttctttgct gtagaaaaaa ataacgcaac tggaaacaga ggaaataaac aatggaaaag
     9841 agaaaaatta ttctggattg tgagccgggt catgatgatg ctattgcaat gatgatggca
     9901 gcgaaacatc cggcaataga tttattaggc atcactattg tagcgggtaa tcagacgctt
     9961 gataaaacat taattaatgg cctgaatgtt tgccagaaac tggagattaa tgttccggtt
    10021 tatgcgggga tgccgcaacc cattatgcgt aaacaaatcg ttgccgataa tattcacggt
    10081 gaaaccggac tggatggccc ggtattcgag ccgctgaccc gccaggcaga aagtacccat
    10141 gcggtgaaat atatcatcga taccctgatg gcaagcgatg gcgatatcac tctggtgccg
    10201 gttggtccgc tttcaaatat cgcggtggca atgcgtatgc aacccgcaat cctgcccaaa
    10261 atccgtgaaa ttgtgctaat gggcggcgct tacggtacag gcaacttcac cccatctgcc
    10321 gagttcaata tctttgccga cccggaagcc gcccgcgtgg tgttcacctc cggcgttcca
    10381 ttagtgatga tgggcctcga tcttaccaac cagaccgttt gcaccccgga tgtaattgct
    10441 cggatggaaa gggtaggcgg ccctgccgga gagctgttca gcgacatcat gaacttcact
    10501 ctcaaaacgc agttcgaata ctacggcctg gctggcggcc cggtgcatga cgccacctgc
    10561 attggttatc tgatcaaccc tgatggcatt aaaacccagg atatgtacgt cgaagtggac
    10621 gtcaacagcg gcccttgcta cgggcgtacg gtctgcgacg aactgggcgt tcttggcaag
    10681 cccgctaata ccaaagtcgg catcactatt gataccgact ggttctgggg cttagtcgaa
    10741 gagtgcgtgc gcggctacat caaaacccat taagtgttga taacaagccg ggacagtatc
    10801 gcaagatgca ggcccggcta tttctgatgg gcagaaagcc gtcgcccaaa atgacatgaa
    10861 gagataatca ctatggatgt catgagaagt gttctgggaa tggtggtact gctggcaatt
    10921 gcgtttttac tgtcagtaaa caagaagaag atcagcctgc gtaccgttgg cgcggcgtta
    10981 gtgttacagg tcgtgattgg cgggattatg ctttggttac cgccagggcg ttgggtcgca
    11041 gaaaaagtcg cttttggcgt ccacaaagtg atggcgtaca gcgacgcagg tagcgcattt
    11101 atcttcggtt ctctggtcgg accgaaaatg gataccttat ttgatggcgc aggatttatc
    11161 tttggtttca gggtattacc ggcaattatc ttcgtcactg cactggtgag tattctctac
    11221 tacatcggtg tgatagggat cttaattcgc attctcggcg gtatcttcca gaaagcttta
    11281 aatatcagca agatcgagtc attcgtcgcg gtcaccacca ttttcctcgt gcaaaacgaa
    11341 attccggcga tcgtcaaacc ctttatcgat cgtctgaatc gcaatgaatt atttacagcg
    11401 atttgtagtg gcatggcctc gattgctggt tcgacaatga ttggttacgc cgcactgggc
    11461 gtacctgtgg aatatttgct ggcggcatcg ttaatggcga tccctggcgg gatcttgttt
    11521 gcccgcctgt taagcccggc tacggaatct tcgcaggttt cttttaataa cctctctttc
    11581 accgaaacac cgccaaaaag cattattgaa gccgatgcga caggggcaat gaccgggctg
    11641 aaaatcgccg ccggtgtagc gacagttgtt atggcatttg tcgccatcat tgcgttaatt
    11701 aacggtatta tcggcggcgt tggcggctgg tttggttttg aacatgcctc gctggagtcc
    11761 attttaggtt acctgctggc cccacttgcg tgggtaatgg gtgttgactg gagtgatgca
    11821 aatcttgccg ggagtttgat tggacagaaa ctggcaataa atgaatttgt cgcttatctc
    11881 aatttctcac cctatctgca aacggctggc actctggatg ctaaaaccgt ggcgattatt
    11941 tccttcgcgt tgtgcggttt cgctaacttt ggttctatcg gggtggtggt gggggcgttt
    12001 tctgcggttg cgccacaccg tgcgccggaa atcgcccagc ttggtttacg cgcgctggcg
    12061 gcggcgacac tttctaatct gatgagtgcc accattgccg ggttctttat tggtttagcg
    12121 tagtgtgcaa tttatgagcc ggattagatg cggtcaagcg tcgaatccgg caacattgtt
    12181 acaccattgg cactaatgac agcgcgttat cggcagacag ggtagggttg tttaagctgc
    12241 cgctggcgcg tgaaattgcc gcgcaggcca tcgcaaaacg ggcgctgtca cggaaattgt
    12301 ttccctccag aaagctatat accagacccg ccataaaacc atcgtcagca ccaaaactgt
    12361 ctaccgtcgt atgcgctggc acagtcagca aaaattgttc accatccttt tcgctgcaat
    12421 aaactgactc atcgggcaaa taaacagttg ctgaacacct tgctgatgca atgcattcac
    12481 tgcggcatta cggtcagcat cgctggtgat cgcctgtccc cataaaattt ccagctccgg
    12541 taaagtgggt ttcagggtgt gaatatgcgc cagccagtgt ttgattttgc ccgctttgaa
    12601 ttctgaaacg gtatcgacaa acaccgggat ctcatcagcg agggtaaaga cccattccag
    12661 cgcctcggct gtcaggttgc aatcaaccag taccacgccc gcatgacgaa gtaaatcgcg
    12721 cgacccgttc agtagttgcg gtgtcaactg ttccagcaga tgggtatcgt taatcgccag
    12781 cacggtttga tcgtctcgat tggcaattgc cagatacgtc gatgtgcttt gaccatgcaa
    12841 acgaacgcag ccggagacat tcacgccggc acggcgcgtt tcttccagga gcatttcgcc
    12901 ataaaagtca tcgccaatca ctgaaagcaa atggacgtca cggcctaaca gcgccagatt
    12961 gtgggcgata ttgcgtccaa cgccgcctgc cgaacaatga attgtaccgg gatgagaagc
    13021 cgcttgcggg taatggatat ccgccatccc gcgaatatcc atattgattg tccccaccac
    13081 tacgcagtat tcctgctcgg tgagaatgta acctttgcct ttaatccggc ctttgcgcat
    13141 taaatccata atatgcgccg caacgcgcga acgactgatt tgcagcatgt ccgcaatttc
    13201 gttctgctga atcagcgggt tacgccgtaa aattgcaagg atctcctttt cccggttatt
    13261 catcttcagg ctaccgcttt ttcagtttgt tgcgctttca gccaggcgat ctcttctgcc
    13321 cagatatccg ggttgatggt ttcaaggatc agcggaatgc cgtcgaaacg gtcgttctgc
    13381 ataatccagc ggaacgcatc atgaccgata ttgccttcac cgaggctatg atggcggtca
    13441 acgcggctgc caaaggtact tttcgcatcg ttaaggtgca tcccgcgcag atacttaaag
    13501 ccgacaatac gggcaaaatc agcgaacgtt ttttcgcatt cggctggagt acgcaaatca
    13561 tacccggcag cgaaagcatg gcaggtatca atgcagacgc cgacgcggga tttatcttcc
    13621 acgccgtcga taatcgccgc gagatgttcg aatttaaacc ctaagttact gccctgaccg
    13681 gcggtgtttt caatcaccgc tgttacacct tgagttttat ccagcgcaat gttgatggat
    13741 tcggcaatac gcgcaaggca atcctcttct gaaatctgca tcagatggct gccagggtgg
    13801 aagttgagca aagaaagccc cagttgttcg caacgctgca tttcatctat aaaggcatcg
    13861 cgcgattttt ccagagcttc agtgaccgga tgtccgaggt tgatcagata actgtcgtgg
    13921 ggaagaattt gcgccgatgt gtagtgatat ttttcacagg cggctttgaa ttcatcgatg
    13981 gtttgcgtcg tgagcggtgc ggcacgccac tgacgttggt ttttggtgaa caaggcaaac
    14041 gcggttgcgt cgatttcggc ggcgcgaatt gcggcatttg ccagaccgcc agcagcacta
    14101 acgtgcgctc caatgtattt catgcgagga ctcctgttaa acccgctgga ggaaaacggt
    14161 aatgatagcg ggttaacagg agcaagaagt agtggtttat gcgatgacgc tttgaatcac
    14221 atagttaatc gcaccaccac caacaatcag ccaggcaaac agtaccagtg ccatcagcag
    14281 aggtttcgcc ccagcttttt tcagcgcgct gacgtgagtg gtcagaccca gcgccgccat
    14341 cgccattgcc agcaggaagg tatccagcgt taccagcatg ttcaccacgc tctgcggtaa
    14401 caggtggaac gagttaaaga tggcaactac gatgaacaag atggcaaacc acggaatagt
    14461 gattttgctt ttctcgccgc tgttcgcccc agacagctgt ttaacacgcg ccgccagcag
    14521 gatgaggaac ggagccagca tcatcacgcg cagcattttg gaaataactg ctgcgttttc
    14581 tgcatccggg ctgatggcat gacctgccgc caccacctgc gccacttcgt gcacagtaga
    14641 accaatgtag ataccgaaag tttccggact aaaccattga gacatcagcg gatatatcgc
    14701 cgggtagagg aaaatcgcga cggtcccgaa gataacaacg gttgcaacag ccacggttac
    14761 tttactggct tccgctttca ctaccggctc agtcgccagt accgcggcag caccacagat
    14821 actgctaccg gcaccgatca accaactggt gtgcttatcc agaccaaaca ctttctgccc
    14881 caggaagcaa gccagcagga aggtactgga cagcgtcagc acgtcaatga tgatcccact
    14941 gataccgaca tcggcaattt gcgagaacgt cagacggaag ccataaagaa tgatacccag
    15001 acgtaataaa tattgcttgg caaacagcac accaccgtca cagcttttcc agatgtgcgg
    15061 atagatggtg ttgcctaaaa ccatccccaa caagattgcg agggtgaggg cactaaaccc
    15121 ggcacccgca accgcgggaa tggaaccacc ccacagggcg accccggtga taactgcact
    15181 cagggctaac cccggaataa aatgccacag tgtacgatgt tgtttctgta aggtgatatt
    15241 cgtcataacc ctctccttta accgggcata aggttacgac taactggttt aaaaataaaa
    15301 ttgattatat atttataatt aatctttata agtggtaagc gactatgcac atcaccctcc
    15361 ggcagttgga agtttttgca gaagtattga aaagtggatc aaccacccag gcgtcggtga
    15421 tgctggcgtt gtcgcaatca gcagtgagcg cagccttgac tgacctggaa gggcagcttg
    15481 gcgttcaact gtttgatcgc gtggggaaaa gactggttgt taatgaacac gggcggctgc
    15541 tctatccgcg agcgttggca ctgcttgaac aggcggttga aatcgaacaa ctgtttcgcg
    15601 aagacaacgg cgcgattcgt atctatgcca gtagtaccat cggtaactac attctgcctg
    15661 cagttatcgc ccgttatcgc catgattatc cgcagttgcc gattgaactt agcgttggga
    15721 acagccagga cgtgatgcaa gcggtgctgg atttccgcgt tgatattggc tttattgaag
    15781 gaccgtgcca cagcactgaa atcatttctg aaccgtggct ggaagacgag ctggtggttt
    15841 tcgccgcgcc gacttcgccg ttggctcgtg gtccggtcac cttagaacag ctggccgctg
    15901 cgccgtggat cctgcgtgaa cgcggttccg gcacgcggga gattgtcgat tatctgttgc
    15961 tgtcacattt accgaagttt gagatggcga tggaattagg taactccgag gcaatcaaac
    16021 atgcggtgcg tcatgggttg ggaattagtt gcctgtcgcg acgtgtgatt gaagatcaat
    16081 tgcaggcagg cacattaagt gaagttgcgg tccctctgcc gcgcctgatg cgtacgttgt
    16141 ggcgtataca tcatcggcaa aaacaccttt ccaacgcgct acggcgcttt ctggactatt
    16201 gcgatcccgc aaatgtgccg cgttaagttg ctgtacaaga acatgctggt gctgtgtcga
    16261 tttcgtgacg cagcgccttc agcatgcatt cgccagaaaa gagattggct gctttactta
    16321 taatccctgg gcgatcatga aggtgtctta taaccgtgta tttctgccgg aaggattgcc
    16381 aatcgtctgc tacaatcgcg ccatcatttt taagatggat agcatttttg tatgggttcc
    16441 gaaactaaaa ctacagaagc gccgggctta cgccgtgaat taaaggcgcg tcacctgacg
    16501 atgattgcca ttggcggttc catcggtaca ggtctttttg ttgcctctgg cgcaacgatt
    16561 tctcaggcag gtccgggcgg ggcattgctc tcgtatatgc tgattggcct gatggtttac
    16621 ttcctgatga ccagtctcgg tgaattggct gcatatatgc cggtttccgg ttcgtttgcc
    16681 acttacggtc agaactatgt tgaagaaggc tttggcttcg cgctgggctg gaactactgg
    16741 tacaactggg cggtgactat tgccgttgac ctggttgcag cacagctggt catgagctgg
    16801 tggttcccgg atacaccggg ctggatctgg agtgcgttgt tcctcggcgt tatcttcctg
    16861 ttgaactaca tctctgttcg cggctttggt gaagcggaat actggttctc actgatcaaa
    16921 gtcacgacag ttattgtctt tatcatcgtt ggcgtgctga tgattatcgg tatcttcaaa
    16981 ggcgcgcagc ctgcgggctg gagcaactgg acaatcggcg aagcgccgtt tgctggtggt
    17041 tttgcggcga tgatcggcgt agctatgatt gtcggcttct ctttccaggg aaccgagctg
    17101 atcggtattg ctgcaggcga gtccgaagat ccggcgaaaa acattccacg cgcggtacgt
    17161 caggtattct ggcgaatcct gttgttctat gtgttagcga tcctgattat cagcctgatt
    17221 attccgtata ccgatccgag cctgctgcgt aacgatgtta aagacatcag cgtcagtccg
    17281 ttcaccctgg tgttccagca cgcgggtctg ctctctgcgg cggcggtgat gaacgcagtt
    17341 attctgacgg cggtgctgtc agcaggtaac tccggtatgt atgcgtctac tcgtatgctg
    17401 tacaccctgg cgtgtgacgg taaagcgccg cgcattttcg ctaaattgtc gcgtggtggc
    17461 gtgccgcgta atgcgctgta tgcgacgacg gtgattgccg gtttgtgctt cctgacctcc
    17521 atgtttggca accagacggt atacctgtgg ctgctgaaca cctccgggat gacgggtttt
    17581 atcgcctggc tggggattgc cattagccac tatcgcttcc gtcgcggtta cgtattgcag
    17641 ggacacgaca ttaacgatct gccgtaccgt tcaggtttct tcccactggg gccgatcttc
    17701 gcattcattc tgtgtctgat tatcactttg ggccagaact acgaagcgtt cctgaaagat
    17761 accattgact ggggcggcgt agcggcaacg tatattggta tcccgctgtt cctgattatt
    17821 tggttcggct acaagctgat taaaggaact cacttcgtac gctacagcga aatgaagttc
    17881 ccgcagaacg ataagaaata agttttctcc cttccttgct aagccctctc aaccgagagg
    17941 gctttttcaa ttccatttcc ctgacaaatc atgcggatat aaaatttaac atttggattg
    18001 ataattgtta tcgtttgcat tatcgttacg acgcaatcaa aaaaggctga caaatcagag
    18061 gctgttccgg ctttctggga tgatcacctg catacaaata agtccaccgc gatgctgccg
    18121 tacgcaaggg gacgtaaaga agatgtgagc gatacccatt ttattttcgt agttacctca
    18181 tggagatatg gaatgtttag gttgaaccct ttcgtacggg tcgggctgtg tttgtccgct
    18241 atttcttgtg catggcctgt gttagcggtc gatgatgatg gcgaaacgat ggttgtcact
    18301 gcatcttccg tggaacaaaa tcttaaagat gcacccgcca gtatcagcgt cattacccag
    18361 gaagacctgc agcgaaaacc ggtacagaat ctgaaggatg tcctcaaaga agtgcctggc
    18421 gtacaactga cgaacgaagg ggataaccgt aagggcgtta gtattcgtgg tctggacagc
    18481 agctacaccc tgattcttgt cgacggtaaa cgcgttaact cccgcaatgc cgtcttccgc
    18541 cacaatgatt tcgatctgaa ctggatcccg gtcgattcca tcgaacgtat tgaagtggtg
    18601 cgtggcccga tgtcgtcgct atacggctcc gatgcgctcg gcggtgtagt gaatatcatc
    18661 accaaaaaaa tcggtcagaa atggtcgggt accgttaccg tcgataccac cattcaggaa
    18721 catcgcgatc gcggtgatac ctataacggt cagttcttta ccagtggacc attaattgat
    18781 ggtgtgctgg gaatgaaagc ttacggcagc ctggcaaaac gtgaaaagga tgacccgcaa
    18841 aactcaacga ccaccgatac cggagaaacg ccgcgtattg aaggattctc cagccgcgac
    18901 ggcaatgtcg aatttgcctg gacaccgaat caaaatcacg attttactgc cggatacggt
    18961 ttcgaccgtc aggatcgtga ttccgactcg ctggacaaaa accgcctgga acgccagaac
    19021 tactgcgtca gccataatgg gcgttgggat tacggcacca gcgaactgaa atcctacggt
    19081 gagaaagtcg agaacaaaaa ccctggcaac agcagcccga taacttccga aagcaatacg
    19141 gtcgacggca aatacacgtt gccgctgacg gcgattaatc agtttctcac ggttggcggt
    19201 gaatggcgtc acgacaaact tagcgatgcg gtgaacctga ccgggggaac cagctccaaa
    19261 acgtctgcca gccagtacgc gctgtttgtg gaagatgaat ggcggatctt cgagccgctg
    19321 gcgctgacga ccggcgtgcg tatggacgat cacgaaacct acggtgaaca ctggagtccg
    19381 cgtgcctacc tggtttataa cgccaccgac accgtaacgg tgaaaggggg ctgggcgacg
    19441 gcatttaaag caccttctct gttgcaactt agccctgact ggacgagcaa ttcctgccgt
    19501 ggcgcatgta agattgtggg tagcccggat ctgaaaccag aaaccagcga aagttgggag
    19561 ctggggcttt actacatggg tgaagaaggc tggctggaag ggggtgaatc cagcgttatc
    19621 gttttccgta acgatgtgaa agatcgtatc agcatcagcc gtacgtctga cgtcaacgct
    19681 gcaccgggct accaaaactt tgttggtttt gagacgggcg ctaacggacg gcgcataccg
    19741 gtatttagct actacagcgt taacaaagct cgtattcagg gcgtggaaac cgaactgaaa
    19801 attccgttca acgatgaatg gaaactgtcg atcaactaca cctacaacga tggtcgtgat
    19861 gtcagcaacg gcgaaaacaa accgctatcc gatctgccgt tccatactgc taacggtacg
    19921 ctggactgga aaccgctggc gctggaagac tggtcattct atgtttctgg tcactatacc
    19981 gggcagaaac gcgccgacag cgcgacggct aaaacaccgg gcggttatac catctggaat
    20041 accggcgcgg cctggcaggt gactaaagac gtcaaactgc gcgcaggcgt gctgaacctt
    20101 ggcgacaagg atctcagtcg tgacgactac agctataacg aagacggacg tcgttacttt
    20161 atggcagtgg attatcgctt ctgatgagaa gatgcccggc gaaccgggcg gactttcact
    20221 tcagtaaata ctgcgcatgg aagcgcaggt gatcctctat aaaagaggcg atgaagtagt
    20281 aactgtgatc atatcccggc tgaatacgca gcgtcatcgg ccacgctttc tggcgcgcgg
    20341 cttctgccaa taccgcaggt tgcaactggt cggcaagaaa ctgatcatta tcgccctgat
    20401 cgataagcgt cgggatcgca tcctgcgcgt tactggcata catcagcgcg caactatccc
    20461 attccagcca tgcatttttg tcctcaccta aataggtgct aaacgctttg attccccacg
    20521 ggacgctgca cggattcaca atgggcgcaa aggccgaaac gctggtgtat ttacccggat
    20581 ttttcagcgc cataatcagc gcaccgtgac cacccattga gtgaccgcta atggcgcagc
    20641 ggtcgctgac attaaattgc gactgaacca gcgccggtaa ttcatcgcgc agataatcat
    20701 acatccggta atacgtcgcc cacggcggtt gcgtggcatt aagataaaag cctgcgccct
    20761 ggcctaaatc gtagccatcg tcgttggcaa ccttttcgcc gcgcgggctg gtgtctggca
    20821 tcaccagtac aatccccagt tccgccgcta cccgctgggc acccgccttg gtggtgaagt
    20881 tctcgtcatt gcaggttaat ccggaaagcc agtacagcac tggtggcggg gtgtgatcac
    20941 gagggggtgg gagaaagata ctgaacgtca tcgggcagtt taaggtactg gagtcgtgtc
    21001 gccagcgttg ctgccagcct tcaaaacagc ggtgctcttc gagcatttcc atgcgtggct
    21061 ccttgttgtg ttgtttgcaa tttcttcatc ataccgttta ttcaatgtgc tgtgagtaac
    21121 tttcacttcc gtatttgcat aacgatgttt taacatctgc tgatgaaagg cagcggcaat
    21181 tacaataatt atcgctgtga atactggatt atgtgcgccg cctcacgcac aataatcaag
    21241 ctgtaaatca gcttaataac tttgccccca cgcagggcgg aggcgtcaca cctgcaggag
    21301 aaatcataaa tgccatcact cagtaaagaa gcggccctgg ttcatgaagc gttagttgcg
    21361 agaggactgg aaacaccgct gcgcccgccc gtgcatgaaa tggataacga aacgcgcaaa
    21421 agccttattg ctggtcatat gaccgaaatc atgcagctgc tgaatctcga cctagctgat
    21481 gacagtttga tggaaacgcc gcatcgcatc gctaaaatgt atgtcgatga aattttctcc
    21541 ggtctggatt acgccaattt cccgaaaatc accctcattg aaaacaaaat gaaggtcgat
    21601 gaaatggtca ccgtgcgcga tatcactctg accagtacct gtgaacacca ttttgttacc
    21661 atcgatggca aagcgacggt ggcttatatc ccgaaagatt cggtgatcgg tctgtcaaaa
    21721 attaaccgta tcgtgcagtt ctttgcccag cgtccgcagg tgcaggaacg tctgacgcag
    21781 caaattctta ttgcgctaca aacgctgctg ggcaccaata acgtggctgt ctcgatcgac
    21841 gcggtgcatt actgcgtgaa ggcgcgtggc atccgcgatg caaccagtgc cacgacaacg
    21901 acctctcttg gtggattgtt caaatccagt cagaatacgc gccacgagtt tctgcgcgct
    21961 gtgcgccatc acaactgatt aaaaggcagg aaccatggag cgcaacgtca cgctcgattt
    22021 tgttcgcggc gtcgccattc tggggatcct gctattaaac atcagcgcct ttgggctacc
    22081 aaaggcggct tatctcaatc ccgcctggta cgatgctatt acgccgcagg atgcatggac
    22141 ctgggcattt ctcgatctca tcggccaggt gaaattcctc acgctttttg cgctgctgtt
    22201 tggtgctggc ctgcaaatgt tgctgccccg tggcagacgc tggatccagt cacggttaac
    22261 gctgttagtc ttgttgggct ttattcacgg tttattgttc tgggacggcg atattctgct
    22321 ggcttacggg ctggtgggct taatctgctg gtggctggtg cgcgatgcgc catcggtaaa
    22381 aagcctgttt aataccggcg tcatgcttta tctggtgggg cttggcgttt tgctgttatt
    22441 ggggctgatt tccgacagcc agaccagccg cgcctggacg ccggatgcat cggctatttt
    22501 gtatgaaaaa tactggaagc ttcacggcgg cgttgaagcg atcagtaatc gtgccgatgg
    22561 tgttggcaac agtttactgg cactgggcgc acagtatggc tggcaactgg cagggatgat
    22621 gctcattggt gcggcgctga tgcgcagcgg ctggctgaaa gggcagttca gcttacgtca
    22681 ctatcgtcgt actggttttg tgctggtggc gattggggtg accattaacc ttcctgccat
    22741 cgccctgcaa tggcagctgg actgggcgta tcgctggtgt gccttcttac ttcaaatgcc
    22801 gcgggaactg agtgcgccgt ttcaggcgat tggctatgcg tcgctgtttt atggcttctg
    22861 gccgcaattg agccgcttta agctggtgct tgcgatcgcc tgcgtcggac ggatggcgct
    22921 gaccaactat ctattgcaaa cgctgatttg taccacgctt ttttaccacc tcggtttgtt
    22981 tatgcatttt gaccgcctgg agctgctggc gtttgttatt ccggtatggc tggcgaatat
    23041 cctcttctct gttatctggc tgcgttactt ccgccagggg ccggtggaat ggctctggcg
    23101 tcagttaact ttgcgtgctg ccggactggc aatatctaaa acatcaagat aacgatctgg
    23161 atcacattcg ttaacaaaac ggctgtaacc gtttccattg ctgtgactcg attcacgaag
    23221 tcctgtattc agtgctgaca aaatagccgc cagcaagcag tcatttactg caatctcata
    23281 acaggtagtg aatatgatca ccattcgtga tgtagcgcgt caggctggcg tctctgtggc
    23341 aacggtttcc cgggtgctca ataacagcac gctggtcagt gccgacacgc gtgaagcagt
    23401 aatgaaagcc gtgagtgagc tggattatcg gccaaacacc aatgcccagg cgctggcaac
    23461 tcaggttagc gacaccattg gcgtggtggt gatggacgtt tctgatgcgt ttttcggcgc
    23521 gctggtaaaa gcggtggatc tggtcgccca gcagcatcag aaatacgtgc taatcggcaa
    23581 tagctatcat gaagcggaaa aagagcgtca cgccattgag gtgttaattc gccagcgttg
    23641 taatgcgttg attgttcact caaaagcatt gagtgacgat gaactggcac aatttatgga
    23701 taacattccc ggtatggtgt taatcaaccg cgttgtgccg gggtacgccc atcgttgcgt
    23761 ttgcctagat aatctcagcg gtgcccgaat ggcgacgcgc atgttgctga ataacggtca
    23821 tcaacgtatt ggttatcttt cttccagcca cggcattgaa gatgacgcca tgcgtaaagc
    23881 aggctggatg agtgcgttga aagagcagga tattattccg ccggaaagct ggattggcac
    23941 tggtacgccg gacatgccgg gcggtgaggc ggcgatggtt gaactgctgg ggcgcaatct
    24001 acaacttacc gctgtatttg cttataacga caatatggcc gctggcgcac tgacagcatt
    24061 aaaagataat ggcattgcga ttccgttaca tctctcaatc atcggtttcg atgatattcc
    24121 catcgcccgt tacaccgacc cgcaattaac gaccgtgcgt tatcccattg cttcaatggc
    24181 taaattagcc accgaactgg ccttgcaggg ggcagcaggc aatattgatc ctcgtgccgc
    24241 cactgtttta tgccgacgtt agtgcgtcgc cattctgtcg caacgcgcca gaatgcggcg
    24301 gcgatcacta actcaacaaa tcaggcgatg taaccgcttt caatctgtga gtgatttcac
    24361 agtatcttaa caatgtgata gctatgattg caccgtttta atgttgtaac ccgtatgtaa
    24421 cagtgaataa tcactttcac cgaggtaata gcgtcataac aacaattaaa gccgttttct
    24481 ggagcgttac cgggcatgga agaacgaatt ttaaaaagtg agcttcggcg ttcagtaaca
    24541 cttcattaac tctactgccc cgccgagcat ttatctcaag cactaccctg cataagaaaa
    24601 accggagata ccatgaataa gaaggtgtta accctgtctg ctgtgatggc cagcatgtta
    24661 ttcggtgccg ctgcacacgc tgctgatact cgcattggtg taacaatcta taagtacgac
    24721 gataacttta tgtctgtagt gcgcaaggct attgagcaag atgcgaaagc cgcgccagat
    24781 gttcagctgc tgatgaatga ttctcagaat gaccagtcca agcagaacga tcagatcgac
    24841 gtattgctgg cgaaaggggt gaaggcactg gcaatcaacc tggttgaccc ggcagctgcg
    24901 ggtacggtga ttgagaaagc gcgtgggcaa aacgtgccgg tggttttctt caacaaagaa
    24961 ccgtctcgta aggcgctgga tagctacgac aaagcctact acgttggcac tgactccaaa
    25021 gagtccggca ttattcaggg cgatttgatt gctaaacact gggcggcgaa tcagggttgg
    25081 gatctgaaca aagacggtca gattcagttc gtactgctga aaggtgaacc gggccatccg
    25141 gatgcagaag cacgtaccac ttacgtgatt aaagaattga acgataaagg catcaaaact
    25201 gaacagttac agttagatac cgcaatgtgg gacaccgctc aggcgaaaga taagatggac
    25261 gcctggctgt ctggcccgaa cgccaacaaa atcgaagtgg ttatcgccaa caacgatgcg
    25321 atggcaatgg gcgcggtaga agcactgaaa gcacacaaca agtccagcat tccggtgttt
    25381 ggcgtcgatg cgctgccaga agcgctggcg ctggtgaaat ccggtgcact ggcgggcaca
    25441 gtactgaacg atgctaacaa ccaggcgaaa gcgacctttg atctggcgaa aaacctggcc
    25501 gatggcaaag gtgcggctga tggcaccaac tggaaaatcg acaacaaagt ggtccgcgta
    25561 ccttatgttg gcgtagataa agacaacttg gctgaattca gcaagaaata agactgattg
    25621 ggcgcagtcg tttactgcgc ccttattgta tggccaacaa ggtaaaatta tatggtcagc
    25681 tcaacgactc cgtcttccgg ggaatacttg ttggaaatga gcggtatcaa caagtccttt
    25741 cctggtgtta aggcacttga taacgttaat ttaaaagtcc ggccacattc tatccatgca
    25801 ttaatggggg aaaacggtgc aggaaaatcg acattattaa aatgcctgtt tggtatttat
    25861 caaaaagact ccggcaccat tttattccag ggtaaagaga tcgatttcca ttccgccaaa
    25921 gaggcactgg aaaatggtat ttcgatggta caccaggagt taaacctggt attacaacgt
    25981 tcggtgatgg ataacatgtg gctggggcga tatcccacca aaggcatgtt tgtcgatcag
    26041 gacaaaatgt accgcgaaac caaagcgatt tttgatgaac tggatattga tatcgatccg
    26101 cgtgcgcgcg tcggcacatt atccgtttcg caaatgcaga tgatcgaaat cgccaaagcg
    26161 ttttcctata acgcgaaaat tgtgattatg gatgaaccga cttcttcgtt aaccgaaaaa
    26221 gaggtcaatc atctgttcac tattattcgt aaattaaaag agcgcggctg cggtattgtt
    26281 tatatctcgc ataaaatgga agaaatcttc cagttatgtg atgaagttac cgtattgcgc
    26341 gacggtcagt ggatcgccac cgaaccgctg gcaggactga cgatggacaa gatcatcggc
    26401 atgatggttg ggcgttctct taatcagcgt tttcctgaca aagaaaacaa gccgggcgaa
    26461 gtcatcctcg aggtgcgtaa cctaacgtca ctgcgccagc cgtcgattcg cgatgtctcg
    26521 tttgatctgc ataaagggga gatcctcggt attgccgggc tggtgggggc gaaacgtacc
    26581 gatattgttg agacgttatt tggtattcgc gagaaatcgg ctggcaccat tacgttgcac
    26641 ggcaaacaga tcaataacca taatgccaac gaagccataa accacggatt tgcactggta
    26701 actgaggagc gccgctcaac gggaatttat gcctatctgg atattggttt taactcgtta
    26761 atttccaata ttcgcaacta caaaaataaa gttggtttac tggataactc gcggatgaaa
    26821 agcgataccc agtgggtgat tgattcgatg cgggtaaaaa cgccgggtca tcggacgcaa
    26881 attggttcgc tctccggtgg taatcagcaa aaggtgatta ttggtcgctg gctactaacg
    26941 caaccagaaa tattaatgct cgatgaaccg acgcgcggta ttgatgtcgg ggcgaagttt
    27001 gaaatttatc agttaattgc cgaactggcg aagaaaggca aggggattat tattatctcc
    27061 tctgaaatgc ctgagttgtt agggataaca gaccgtattc tggtcatgag caatggtctc
    27121 gtttccggaa ttgtcgatac aaaaacaaca acgcaaaacg aaattctgcg tcttgcgtct
    27181 ttgcaccttt aagattaggg gcttcccatg agtgcgttaa ataagaaaag ttttcttact
    27241 tacctgaaag agggcggtat ttacgtcgtt cttttagttt tgctggcgat tattattttc
    27301 caggacccaa catttttaag tctgttgaac ttaagtaata ttctcaccca gtcatcggtg
    27361 cgtattatta tcgcgctcgg tgtggcaggg ttaattgtca cccaggggac cgatctttct
    27421 gctggtcgtc aggtagggct ggcggcagtg gtggctgcga cattattgca gtccatggat
    27481 aacgccaaca aagtgttccc ggaaatggcg acgatgccga ttgcgctggt tattctgatt
    27541 gtctgtgcca ttggtgcggt gatcggtttg atcaacggtc tgattatcgc ttatctcaac
    27601 gtgacgccgt tcattaccac gctcggcacg atgatcatcg tctatggcat caactcgctc
    27661 tattacgact ttgtcggggc gtcgccaatt tctggttttg acagtggctt ctctaccttt
    27721 gctcagggct ttgtcgcgct ggggagtttc cgtctctctt acatcacctt ctacgcgttg
    27781 attgcggtgg cgttcgtctg ggtgttgtgg aacaaaaccc gcttcggtaa gaacattttt
    27841 gccattggcg gtaacccgga agcggcaaaa gtatctggtg tcaacgtcgg cctgaacctg
    27901 ctgatgatct acgcgttgtc tggcgtgttc tatgcctttg gcgggatgtt agaagccgga
    27961 cgtatcggct ctgccaccaa caacctcggc tttatgtatg agctggatgc tatcgcggcg
    28021 tgcgtggtag gcggtgtatc gttcagcggc ggtgtgggga cggtgattgg cgtggtgacc
    28081 ggggtaatta tttttaccgt catcaactat ggcctgacgt atatcggcgt aaacccatac
    28141 tggcagtaca tcatcaaagg ggcgattatt atcttcgccg tagcgctgga ttcactgaaa
    28201 tacgcgcgta agaagtgatg tgattgcgga tattgccgga tgcgtctctg gttttacaac
    28261 gttaccgggt gttctttctc gcctttctta aacttcactt ccccgagctc aatacaaccc
    28321 accgggcaga cgtgaccaca aagcagacaa cccacacatt tctcggtatt acaatgcggt
    28381 gtgcgggttt tctcgctcca ttccatcgcc tggtgaccgc cgtcgtaaca ggaaatataa
    28441 cagcgtccac agccaacaca tttgtcaaga ttgatacggg gatagacaat ataactgcgg
    28501 tctaaatctt ccgccgggac aatattgtta ttcgccagac ctaccatctc ctgcaacgaa
    28561 tcaaatccct gatcggcgag ataatgcgat aacccgctcg ccatatcttc tacgatgcga
    28621 tagccgtact gcataatgcc ggtggtgacc tgtaacgttg ctgcgccgag caataaaaac
    28681 tccgccgcat cttcccaggt ttcaatgccg ccgataccgc tgattgggaa atcgcgcagt
    28741 tctggatggg tgcgcatttg ctgaatgaag cgcagggcga tcggttttac cgctttaccg
    28801 gaatatccgg aaatactcga tttaccgtta acgatcggca taccgatttt ctgattaaga
    28861 tcgatattgg tgatggattt aacggtgtta attgccgcaa tgccatctgc gccgccgcgc
    28921 ttcgccgcca gcgccacttc gcacatatca ccgatattcg gcgtcatctt cgccagcatt
    28981 ggcagcgtgg aaccccgttt cactgcccga caatattttt ttaccagctc cgggctttgc
    29041 ccgacatcgc tacccatcgc atgagaagtc atttgcggac aggagaagtt acactcgatc
    29101 atatccgcgc cagcttcttg caccaggcgc gccagctcct cccattgctg ctcattttcc
    29161 cccatgatcg aagcgatcaa tactttgtcc gggtaatctt ccttcagccg acgcagggcg
    29221 gtcagattct cttccaacgg atgttcagca atctgctcca tatttttgaa gccgataaaa
    29281 ccggtatctt ctttcaccag atgatcaaaa cgcggcgaga cttcgttggc gataaaaaag
    29341 ccgatcgttt taaacaccac accgccccaa cctgtgtcgt aggctttggc acacatctca
    29401 tagcagttgc ctaccggcga agaagagagg cagaacgggt tgggaaagtt cacgccgcaa
    29461 aaagtaatcg aaagatcttt cgttaacatg agcaagctcc ctctaatata gtgatgaatc
    29521 gcctcggcgg cttctttccc ggttttcacg gcatagacca cggttttgtc accctcaaca
    29581 atatcgccag cagcaaagac ttgcggatcg cgggtctgat aattttgtgt tttgatggtg
    29641 ttacgctgct gctctaactc cgcaaaggca tccagtctgg catgctgacc gacggcgaga
    29701 ataattttat ctgccgccat cgtcagttcg cctgataacc gtacatgctt aaacgtgact
    29761 ttattgcctt ccacggctac tggcgtgaat ccatcgatga tcgaaacgcc cagctcccgg
    29821 gcgctggcaa actctttttc gcttgccgga aactcatcta actcttcacg tgctacgcaa
    29881 gttaccgcct gacagccgag aactttcagc gtgctggcta cgtccatcgc gacatcaccg
    29941 ccgccgataa ttaatgcgct ttgtggaatg ctgatatcgc cttgcgcctg tcgtacacgt
    30001 tgcaggaagt cgacggcaat ctcaacgtcg ctatgctcaa acagcggtag cccggaaccg
    30061 cttgataacc ccacggtgac cagtaccgcg cggttttccg ctttcagttg ctccagggtg
    30121 agtgttttac cgatttcgtt attgcactta atggtcacgc ccattttttc aatacgggcg
    30181 atctctgcat ccagcactga ttgcggtaac cggaattgcg gaataccgtt acgcaaccag
    30241 ccaccggggt gcgcttcttt ctcataaatc gtgacgtcat aaccctggtt tgtcagtgtc
    30301 acactggcct gcaatccggc aggacctgcg ccaataatcg cgactttgcc gagcgtttta
    30361 gtaccgggct gataaatttc catcccggtt tgttgttcaa aatcagtaac aaaacgctgt
    30421 aagcggccga tatcaatggg agcatcaaca ccggcacggg tacaaccgct ttggcataat
    30481 ttctccgtcg ggcacactct ggcacaaacg gcaccgaggg cattattttc gcgaattgtc
    30541 tcggcagcgc ctttaaaatt acgaaagtag attgagcgaa taaatttccc cggatcggtc
    30601 tgtgccgggc aagcctgact acagggagcg tcgtgacata ataaacagcg agaggcttct
    30661 ttaatcgcca gtaacggcgt aaaagccgga gtgagttcat ccagataatt ttgttgcggc
    30721 atgtgatctt ccttaatgct atgagtaatt atggcgaacg ctaaataaat attgcgtgct
    30781 gatattaagg ctatgcgcgt ggggaaattt aaactgtgaa aggattcaca aatagctgcg
    30841 gttcagaatg ttatatccgc agcttattga gtgaagtaat aattattctc aataattaac
    30901 agaaaagaga aaatattaac gatagaaaat aagataaaca atcatgccga tagataaaat
    30961 tccgaagcca atatatgcca ccatataagc gaagaagagt ccgggatttt ttgctcgcca
    31021 gacatacacc ggttccggtg catttttaac ccgtttatca atcgcgcctg cgcgccagcc
    31081 tttccatgct tcgcggaaac aaaatatcgg gataaggaaa aaaacggcaa cgacaaaaaa
    31141 ctgctgtaca tccataacta ctttccttgt ttcttttgta attcaagtaa ctgttcgggt
    31201 gtaacggcgg gatacccctg cgcatcttcc ggcacctggt gcatagccgg aatagggacc
    31261 agcggcccta aaaaacgcgg ttcacgttta aaaatataaa ggtcagccag cgcaccgaaa
    31321 cgggcggcaa attcgcgaat acgtactgac agcatatctt tcggtgacgg tacggcataa
    31381 cactgagcct gaatccccat atgcagcgca ataaataatg ctcgctcaca gtggaaacgt
    31441 tgggtgataa taatgaaatc attagtatcg aaaactttgc gtgtacgcac gatggaatcc
    31501 agcgtacgaa agcctgcgta atcgagaaca atatctgatg ggtcgacacc agcagcgatt
    31561 aaatctttgc gcatggtcat cggctcgtta taactttgca atgcgttatc gccgctcagt
    31621 aatagataat ttaccttacc gctgttatag gcattaatcg ctccttgaat gcggtagcga
    31681 taatactgat taataacgcc ggtacgataa tattttgctg tgccgagcac cacgccgacc
    31741 tggcggtagg ggagatcctg caattcgtcg tagatataag gcgcggtttt ccagctcatc
    31801 cagcgatcga ggccgagcac agtcaacagc agcaagccga tcaggactaa caggctgagg
    31861 aacacgcgct ttaacatgca ggagactcga gctaaggaaa atttccatca ggctacttta
    31921 cccgctggtt ttgcgcaaga aacggcaaac tgtttgccgg gaatttcgac accaggagag
    31981 ggctggcaag ccggacaagg cgttcacgcc gcatccggca ccaggcttaa gcgagaagca
    32041 cgcggtcgat actgtgacag ccgagagctt tcagcgttgc ggaggtggca tcccactgaa
    32101 tcaacggcgc atcggctttt tctgccagaa ccgcgcgctg gatatccggg taatcataac
    32161 ccttgagatt caacagaatt aacgccccct gcaacggcgg cagagtcggg ttgaatgcgg
    32221 cgttttcagc gtagctgccg ctgaaaatac gaccgtcttt acattccagc gcgacaccgc
    32281 ttggtgactt actgtaaggc atgtgcgaac ggtttgccgc tgcaatcgcc gcctgagaaa
    32341 gcgcatcacc agtcagcgca tagccgtgat cctgctcgtc catcagcagc gttttaatct
    32401 ccagatcttt cggcccaaag gcatccggca gatagtcacg cagcgcgtgt gcctcgcggc
    32461 ccggcaaatg aatacgcaga tccagaccgc tgttcagttc attcataaac tgacggcagt
    32521 gaccacaagg cgtatagtta acggtgatgg ctgcaagcgc tttttcacca ctcaaccagg
    32581 cgtggctgat cgcgctttgt tcggcatgaa cggtttgctg cattgttgca ccaataaatt
    32641 ccatattggc accaaaatac caggttccgc tcacaccgcg cgcaattgcg ccaacattaa
    32701 aattcgacaa tggcgtacgc gcacaggccg ccgccagcgg aagtagtgcg aatgccagcg
    32761 cgtcttcgtc cagccccgtt gcgctcttca gcgatgagac ttgctccccg gtcaacaaag
    32821 cggggaagta cttgtctgcc agaataggtt ccagtgcaga ttgcaagtta tccgcaagtt
    32881 gggcaaaagc ggtttgaaaa cgtggatgca tggcgttgcc tcataatcat gtaatgggat
    32941 aacagtttac gaacccgtta tggctttata tgtgatctga atctcattaa ttatgcaaca
    33001 cgtgaaactt acatcaaaaa tgcgcgacgt atcgcaaatt ttagcccatt actgccagaa
    33061 taatcgggaa aaggaacggt gcgatcagcg aagtaattat cccgcataac accagcgcta
    33121 gcgaactaaa tgcaccttcc tgataatcca gctcggcgca gcgcgccgta ccgagggcgt
    33181 gcgaggcagt ccccattgcc agaccgcgcg cagctttggt acgaatacgc atcgcattaa
    33241 gcaatgtatg gccaaatacc gcgccgagga tgccgacgaa aatcacgcaa actgcgctga
    33301 ttgccggaat accgccaata ctgccgccaa ccgacattgc aataggcgtg gtgactgatt
    33361 tcggcaggat tgacgcggtg atttccggtg aagcgcccat caacaatgcc acggaagtcc
    33421 ccgtcaccat cgcaaccacg ctgccgataa aacagatggt gatgatcgat ttccagcgcg
    33481 cgcggatctg gtgtagctgc tcatataaag gataggccag cgcgacgacc gccggttgca
    33541 gcagatcgtt tagcacttcg ctacctttaa agtaactgtc gtaagagatg ccggtcagca
    33601 ttaaaaaagg aatgatcacc accattgcta ccagcaacgg gttaagcagg ggaaacttat
    33661 accgtgccgc cagtttgcgg gcggcaaaaa agacaatcaa cgtcagcggt aatgaccacc
    33721 agatattcgc catcattctt ctgatccttt ctgacctacg actttacgtt caccgtgtac
    33781 cagttgtgaa ctccagctca ccaccagaaa aaccaccagc gtactgacag cacaggaaac
    33841 cactaccggg ccaaactgcg cgcggagcaa atcaaaatat tgcatgacgc ctacgccaat
    33901 cggcacaaac aatagcgcca tatagcgaat cagtacgtag caccccggat tgacccattt
    33961 cgccggaaga atttgcaagg ccagcaggac aaacaggatc agcatcccga tgatgctgcc
    34021 cggaatggtt accggtagca gggaagcaat gaaaatgcct gcatacaggc aggcataaat
    34081 caggacgaaa gcgcgtaaat attgccagat aatgttcagt gtcttactca taatcaacac
    34141 cccagatgag atggattcat catacaatta attcgtaaaa cgtgctaccg atcacattaa
    34201 gaaatcagcg cttatttttt cgctacccgg tattacgcaa tgagcagcga actcaggcat
    34261 acatcaagca tagagaggga ttgcagtttg cagcaatccc tctttgcaga cctttaatcc
    34321 gctgaccata ggcgctaaca tagcgcctca tttttttgcg ggtgatgata tacgtgtgtt
    34381 actggcaccg atggagggag tgcttgactc tctggtgcgt gaattgctga ccgaagttaa
    34441 cgactacgat ctgtgcatca ccgagtttgt ccgcgtggtg gatcaactgc tgccggtaaa
    34501 agtctttcat cgcatttgcc ctgagctaca aaacgccagc cggacaccat ctggtacgct
    34561 ggtgcgcgtg cagttgttag gtcagttccc acaatggctg gcagagaacg ccgcccgtgc
    34621 ggtggagtta ggttcctggg gcgtggatct caattgcggc tgcccgtcga aaacggttaa
    34681 cggtagcggc ggcggggcga cgttactcaa agatcctgaa ctcatctacc agggggcaaa
    34741 agcgatgcgt gaagctgtac cggcgcattt gcctgtcagc gtgaaagtgc gtctgggctg
    34801 ggacagcggt gagaagaaat ttgaaatcgc cgatgcggtt caacaggctg gcgctacgga
    34861 gctggtggtg catgggcgga cgaaagagca gggttaccgc gcggagcata ttgactggca
    34921 ggcgattggc gagattcgcc agcggctgaa tattccggtg attgccaacg gtgaaatctg
    34981 ggactggcag agcgcgcaac aatgcatggc gatcagcggc tgcgacgcag tgatgattgg
    35041 tcgcggggcg ctcaatattc ccaacctgag ccgggtggta aaatataacg aaccgcgaat
    35101 gccgtggccg gaggtggttg ctttgctgca aaaatatacc cgtctggaaa agcagggcga
    35161 taccgggtta tatcacgttg cgcggattaa acagtggttg agttatttgc gtaaagaata
    35221 cgatgaagca acggaattat ttcagcatgt tcg
//
LOCUS       DAETPZ010000037        35098 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.037, whole genome shotgun sequence.
ACCESSION   DAETPZ010000037 DAETPZ010000000
VERSION     DAETPZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.037"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            88..1116
                     /gene="murB"
                     /locus_tag="K0W59_002012"
     CDS             88..1116
                     /gene="murB"
                     /locus_tag="K0W59_002012"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBI3023049.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            1113..2078
                     /gene="birA"
                     /locus_tag="K0W59_002013"
     CDS             1113..2078
                     /gene="birA"
                     /locus_tag="K0W59_002013"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HBI3023050.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGSGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(2107..3057)
                     /gene="coaA"
                     /locus_tag="K0W59_002014"
                     /gene_synonym="panK"
     CDS             complement(2107..3057)
                     /gene="coaA"
                     /locus_tag="K0W59_002014"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBI3023051.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            3419..3494
                     /locus_tag="K0W59_002015"
     tRNA            3419..3494
                     /locus_tag="K0W59_002015"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3452..3454,aa:Thr,seq:tgt)
     gene            3503..3587
                     /locus_tag="K0W59_002016"
     tRNA            3503..3587
                     /locus_tag="K0W59_002016"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3537..3539,aa:Tyr,seq:gta)
     gene            3704..3778
                     /locus_tag="K0W59_002017"
     tRNA            3704..3778
                     /locus_tag="K0W59_002017"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3737..3739,aa:Gly,seq:tcc)
     gene            3785..3860
                     /locus_tag="K0W59_002018"
     tRNA            3785..3860
                     /locus_tag="K0W59_002018"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3818..3820,aa:Thr,seq:ggt)
     gene            3975..5159
                     /gene="tuf"
                     /locus_tag="K0W59_002019"
     CDS             3975..5159
                     /gene="tuf"
                     /locus_tag="K0W59_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBI3023052.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVG
                     VLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFR
                     TTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKV
                     LS"
     gene            5389..5772
                     /gene="secE"
                     /locus_tag="K0W59_002020"
     CDS             5389..5772
                     /gene="secE"
                     /locus_tag="K0W59_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBI3023053.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            5774..6319
                     /gene="nusG"
                     /locus_tag="K0W59_002021"
     CDS             5774..6319
                     /gene="nusG"
                     /locus_tag="K0W59_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBI3023054.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            6478..6906
                     /gene="rplK"
                     /locus_tag="K0W59_002022"
     CDS             6478..6906
                     /gene="rplK"
                     /locus_tag="K0W59_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBI3023055.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            6910..7614
                     /gene="rplA"
                     /locus_tag="K0W59_002023"
     CDS             6910..7614
                     /gene="rplA"
                     /locus_tag="K0W59_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBI3023056.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            8027..8524
                     /gene="rplJ"
                     /locus_tag="K0W59_002024"
     CDS             8027..8524
                     /gene="rplJ"
                     /locus_tag="K0W59_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBI3023057.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            8591..8956
                     /gene="rplL"
                     /locus_tag="K0W59_002025"
     CDS             8591..8956
                     /gene="rplL"
                     /locus_tag="K0W59_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBI3023058.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            9276..13304
                     /gene="rpoB"
                     /locus_tag="K0W59_002026"
     CDS             9276..13304
                     /gene="rpoB"
                     /locus_tag="K0W59_002026"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBI3023059.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EGGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            13381..17604
                     /gene="rpoC"
                     /locus_tag="K0W59_002027"
     CDS             13381..17604
                     /gene="rpoC"
                     /locus_tag="K0W59_002027"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBI3023060.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(17846..18979)
                     /gene="thiH"
                     /locus_tag="K0W59_002028"
     CDS             complement(17846..18979)
                     /gene="thiH"
                     /locus_tag="K0W59_002028"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBI3023061.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANNCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(18976..19746)
                     /gene="thiG"
                     /locus_tag="K0W59_002029"
     CDS             complement(18976..19746)
                     /gene="thiG"
                     /locus_tag="K0W59_002029"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBI3023062.1"
                     /translation="MLRIVDKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(19748..19948)
                     /gene="thiS"
                     /locus_tag="K0W59_002030"
     CDS             complement(19748..19948)
                     /gene="thiS"
                     /locus_tag="K0W59_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBI3023063.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(19932..20687)
                     /gene="thiF"
                     /locus_tag="K0W59_002031"
     CDS             complement(19932..20687)
                     /gene="thiF"
                     /locus_tag="K0W59_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HBI3023064.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(20680..21315)
                     /gene="thiE"
                     /locus_tag="K0W59_002032"
     CDS             complement(20680..21315)
                     /gene="thiE"
                     /locus_tag="K0W59_002032"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBI3023065.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(21315..23210)
                     /gene="thiC"
                     /locus_tag="K0W59_002033"
     CDS             complement(21315..23210)
                     /gene="thiC"
                     /locus_tag="K0W59_002033"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBI3023066.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(23291..23391)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(23444..23920)
                     /gene="rsd"
                     /locus_tag="K0W59_002034"
     CDS             complement(23444..23920)
                     /gene="rsd"
                     /locus_tag="K0W59_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HBI3023067.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            24015..24788
                     /gene="nudC"
                     /locus_tag="K0W59_002035"
     CDS             24015..24788
                     /gene="nudC"
                     /locus_tag="K0W59_002035"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HBI3023068.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            24828..25892
                     /gene="hemE"
                     /locus_tag="K0W59_002036"
     CDS             24828..25892
                     /gene="hemE"
                     /locus_tag="K0W59_002036"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HBI3023069.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            26049..26720
                     /gene="nfi"
                     /locus_tag="K0W59_002037"
     CDS             26049..26720
                     /gene="nfi"
                     /locus_tag="K0W59_002037"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HBI3023070.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            26763..27353
                     /locus_tag="K0W59_002038"
     CDS             26763..27353
                     /locus_tag="K0W59_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HBI3023071.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            27540..27812
                     /gene="hupA"
                     /locus_tag="K0W59_002039"
     CDS             27540..27812
                     /gene="hupA"
                     /locus_tag="K0W59_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HBI3023072.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            27825..28520
                     /locus_tag="K0W59_002040"
     CDS             27825..28520
                     /locus_tag="K0W59_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HBI3023073.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(28522..28947)
                     /gene="zraP"
                     /locus_tag="K0W59_002041"
     CDS             complement(28522..28947)
                     /gene="zraP"
                     /locus_tag="K0W59_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HBI3023074.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            29185..30561
                     /gene="zraS"
                     /locus_tag="K0W59_002042"
     CDS             29185..30561
                     /gene="zraS"
                     /locus_tag="K0W59_002042"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HBI3023075.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            30558..31883
                     /gene="zraR"
                     /locus_tag="K0W59_002043"
     CDS             30558..31883
                     /gene="zraR"
                     /locus_tag="K0W59_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HBI3023076.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(31880..33169)
                     /gene="purD"
                     /locus_tag="K0W59_002044"
     CDS             complement(31880..33169)
                     /gene="purD"
                     /locus_tag="K0W59_002044"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBI3023077.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(33181..34770)
                     /gene="purH"
                     /locus_tag="K0W59_002045"
     CDS             complement(33181..34770)
                     /gene="purH"
                     /locus_tag="K0W59_002045"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBI3023078.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         8364 a         8986 c         9557 g         8191 t
ORIGIN      
        1 ctacaaactc ttcctgtcgt catatctaca agccatcccc ccacagatac ggtaaactag
       61 cctcgttttt gcatcaggaa agcagctatg aaccactcct taaaaccctg gaacacattt
      121 ggcattgatc ataatgctca gcacattgta tgtgccgaag acgaacaaca attactcaat
      181 gcctggcagc atgcaaccgc agaaggacaa cccgttctta ttctgggtga aggaagtaat
      241 gtactttttc tggaagacta tcgcggcacg gtgatcatca accggatcaa aggtatcgaa
      301 attcatgatg aacctgatgc gtggtattta catgtaggag ccggagaaaa ctggcatcgc
      361 ctggtaaaat acactttgca ggaaggtatg cctggtctgg aaaatctggc attaattcct
      421 ggttgtgtcg gctcatcacc tatccagaat attggtgctt atggcgtaga attacagcga
      481 gtttgcgctt atgttgattg tgttgaactg gcgacaggca agcaagtgcg cttaactgcc
      541 aaagagtgcc gttttggcta tcgcgacagt atttttaaac atgaatacca ggatcgcttc
      601 gccattgtag ccgtaggtct gcgtctgcca aaagagtggc aacctgtact aacgtatggt
      661 gacttaactc gtctggatcc tacaacagta acgccacagc aagtatttaa tgcggtgtgt
      721 catatgcgca ccaccaaact ccctgatcca aaagtgaatg gcaatgccgg tagtttcttc
      781 aaaaaccctg ttgtatctgc cgaaacggct aaagcattac tggcgcaatt tccaacagca
      841 ccaaattacc cccaggcggg tggttcagta aaactggcag caggttggct tattgatcag
      901 tgccagctaa aagggatgca aatgggtggg gctgcggtgc accgtcaaca ggcgttagtt
      961 ctcattaatg aagacaatgc aaaaagcgaa gatgtggtgc aactggcaca ccatgtaaga
     1021 caaaaagtgg gtgaaaaatt taatgtctgg cttgagcctg aagtccgctt tattggtgca
     1081 tcaggtgaag tgagcgcagt ggagacaatt tcatgaagga taacaccgtg ccactgaaat
     1141 tgattgccct gttagcgaac ggtgaatttc actctggcga gcagttgggt gaaacgctgg
     1201 gaatgagccg ggcggctatt aataaacaca ttcagacact gcgtgactgg ggcgttgatg
     1261 tctttaccgt tccgggtaaa ggatacagcc tgcctgagcc catccagtta cttaatgctg
     1321 aacagatatt gggtcagctg gatggcggta gtgtagccgt gctgccagtt attgactcca
     1381 cgaatcagtt ccttcttgat cgtatcggag agcttaaatc gggcgatgcc tgtgttgcag
     1441 aataccagca ggctggccgt ggtcgccggg ggcggaaatg gttttcgcct tttggcgcaa
     1501 acttatattt gtcgatgttc tggcgtctgg aacaaggccc ggcggcggcg attggtttaa
     1561 gtctggttat cggtatcgtg atggcggaag tattacgaaa gctgggagca gataaagttc
     1621 gtgtcaaatg gcctaatgac ctctatctgc aggatcgcaa gctggcaggc attcttgtgg
     1681 agctgactgg caaaactggc gatgcggcgc aaatagtcat tggatccggg atcaacatgg
     1741 caatgcgccg tgttgaagag agtgtcgtta atcaggggtg gatcacgctg caggaagcgg
     1801 ggatcaatct tgatcgtaat acgttggcgg ccatgctaat acgtgaatta cgtgctgcgt
     1861 tggaactctt cgaacaagaa ggattggcac cttatctgtc gcgctgggaa aagctggata
     1921 attttattaa tcgcccagtg aaacttatca ttggtgataa agaaatattt ggcatttcac
     1981 gcggaataga caaacagggg gctttattac ttgagcagga tggaataata aaaccctgga
     2041 tgggcggtga aatatccctg cgtagtgcag aaaaataaga aaggggagta ttcgctcccc
     2101 tgcaaattat ttgcgtagtc tgacctcttc taccacatga ttagcacttt tcgtcaggat
     2161 taaactggcg cgctcacgag taggtagaat attttgcttt aagttcagcc agttgatctc
     2221 tttccacaat gtcatggcag tcttaatcgc ttcttcttta gttaatttcg cgtagttatg
     2281 aaaataggaa tccgggtctg taaaagcccc ttcgcggaat ttcagaaaac ggttgatata
     2341 ccacgtctga agtaagtctt ccggtgcatc aacatatatc gaaaaatcga caaaatcgga
     2401 aacaaataca tgatgtggat cgtgtggata atccatcccg ctctgtaaga catttaaccc
     2461 ttcaagaatt aaaatatcag gctgaacaac agttttatct ccatccggga tcacatcata
     2521 aataagatgc gagtaaacag gggctgtaac gtttggcacg ccggatttga gatcggaaac
     2581 aaacttcacc aggcgatgca tatcatacga ttccgggaag cctttcttct tcatcagacc
     2641 acgttctttc agaacctgat tagggtgaag gaagccatct gtagtgatca gttcaacacg
     2701 acgatgttcc ggccaacggc ttaatagcgc ctgcaataca cgggcggttg tacttttccc
     2761 caccgcgaca ctgccagcaa tactgataat gtaaggaatg cgttgcccgt tggtaccaag
     2821 aaactgttcc agaactgcct gacggcgcag attcgagctt atatagaagt tcagcaaacg
     2881 tgacaaaggt aaatagatct cggcaacttc ttctaacgag agatcttcat taataccttt
     2941 gagacgggcg atctcatctt ccgataacgt cataggtacg gaatcacgca gagctgccca
     3001 ctggttgcgg tcaaactgta ggtaaggcgt cattaacgtt tgctctttta tactcataag
     3061 catgtttctg gcggtcatag ctctatcggc aaagcgatat ttgccatggc gcgtcacagt
     3121 tctgttgaat gggttaatgg gcaggagggt aacaccagat ggggaggaat aataagaaaa
     3181 aatctctgcc aggaagctat cgttgaaaag caatgtgaca gatattccat ctgataaaaa
     3241 gaattatggt ttagcaggag cgcattgttg agcacaatga tgttgaaaaa gtgtgctaat
     3301 ctgccctccg ttcggctgtt tcttcatcgt gtagcataaa atgtgaccaa taaaacaaat
     3361 tgtgcaattt tttagttgca tgaactcgca tgtctccata gaatgcgcgc tacttgatgc
     3421 cgacttagct cagtaggtag agcaactgac ttgtaatcag taggtcacca gttcgattcc
     3481 ggtagtcggc accatcaagt ccggtggggt tcccgagcgg ccaaagggag cagactgtaa
     3541 atctgccgtc acagacttcg aaggttcgaa tccttccccc accaccaatt tcggccacgc
     3601 gatggcgtag cccgagacga taagttcact taccggctcg aataaagaga gcttctctcg
     3661 atattcagtg cagaatgaaa atcaggtagc cgagttctag gatgcgggca tcgtataatg
     3721 gctattacct cagccttcca agctgatgat gcgggttcga ttcccgctgc ccgctccaag
     3781 atgtgctgat atagctcagt tggtagagcg tacccttggt aagggtgagg tcggcagttc
     3841 gaatctgcct atcagcacca cttcttttct cctccctgtt ttttccttct gtttattgca
     3901 ttcaacaagt cgggcatgtt gcctggttga tgtggtgata tcaccgattt atccgtgtct
     3961 tagagggaca atcgatgtct aaagaaaagt ttgaacgtac aaaaccgcac gttaacgtcg
     4021 gtactatcgg ccacgttgac catggtaaaa caacgctgac cgctgcaatc actaccgtac
     4081 tggctaaaac ctacggcggt gctgctcgcg cattcgacca gatcgataac gcgccggaag
     4141 aaaaagctcg tggtatcacc atcaacactt ctcacgttga atacgacacc ccgacccgtc
     4201 actacgcaca cgtagactgc ccggggcacg ccgactatgt taaaaacatg atcaccggtg
     4261 ctgctcagat ggacggcgcg atcctggtag ttgctgcgac tgacggcccg atgccgcaga
     4321 ctcgtgagca catcctgctg gggcgtcagg taggcgttcc gtacatcatc gtgttcctga
     4381 acaaatgcga catggttgat gacgaagagc tgctggaact ggttgaaatg gaagttcgtg
     4441 aacttctgtc tcagtacgac ttcccgggcg acgacactcc gatcgttcgt ggttctgctc
     4501 tgaaagcgct ggaaggcgac gcagagtggg aagcgaaaat cctggaactg gctggcttcc
     4561 tggattctta cattccggaa ccagagcgtg cgattgacaa gccgttcctg ctgccgatcg
     4621 aagacgtatt ctccatctcc ggtcgtggta ccgttgttac cggtcgtgta gaacgcggta
     4681 tcatcaaagt tggtgaagaa gttgaaatcg ttggtatcaa agagactcag aagtctacct
     4741 gtactggcgt tgaaatgttc cgcaaactgc tggacgaagg gcgtgctggt gagaacgtag
     4801 gtgttctgct gcgtggtatc aaacgtgaag aaatcgaacg tggtcaggta ctggctaagc
     4861 cgggcaccat caagccgcac accaagttcg aatctgaagt gtacattctg tccaaagatg
     4921 aaggcggtcg tcatactccg ttcttcaaag gctaccgtcc gcagttctac ttccgtacta
     4981 ctgacgtgac tggtaccatc gaactgccgg aaggcgtaga gatggtaatg ccgggcgaca
     5041 acatcaaaat ggttgttacc ctgatccacc cgatcgcgat ggacgacggt ctgcgtttcg
     5101 caatccgtga aggcggccgt accgttggcg cgggcgttgt agcaaaagtt ctgagctaat
     5161 tgccgataac atttgacgca atgcgcacta aaagggcatc atttgatgcc ctttttgcac
     5221 gctttcgtac cagaacctgg ctcatcagtg attttctttg tcataatcat tgctgagaca
     5281 ggctctgttg agggcgtata atccgaaaag ctaatacgcg tttcgatttg gtttgcctcg
     5341 cgatcgcggg gtgaaaatgt ttgtagaaaa cttctgacag gttggtttat gagtgcgaat
     5401 accgaagctc aaggaagcgg gcgcggcctg gaagcgatga agtgggtcgt tgtggtggca
     5461 ttgctcctgg cggcgattgt cggcaactac ctttatcgcg acattatgct gccgctgcgt
     5521 gcgctggccg tagtaattct gattgctgca gcgggtggtg tcgcgctgtt aacgacaaaa
     5581 ggtaaagcta ccgttgcttt tgcccgtgaa gcgcgtaccg aagtccgtaa ggtcatttgg
     5641 ccgactcgcc aggaaacatt gcacaccacg ctgattgtgg ctgcggttac cgcagtaatg
     5701 tcactgatcc tgtggggact ggatggtatt ctggttcgcc tggtatcctt tatcactggc
     5761 ctgaggttct gagatgtctg aagctcctaa aaagcgctgg tacgtcgttc aggcgttttc
     5821 cggttttgaa ggccgcgtag caacgtcgct gcgtgagcat atcaaattac acaacatgga
     5881 agatttgttt ggtgaagtca tggtaccaac cgaagaagtg gttgaaatcc gtggcggtca
     5941 gcgtcgcaaa agcgaacgta aattcttccc tggctacgtc ctcgttcaga tggtgatgaa
     6001 cgacgcgagc tggcacttgg tgcgcagcgt accgcgtgtg atgggcttca tcggcggtac
     6061 ttccgatcgt cctgcgccaa tcagcgataa agaagtcgat gcgattatga accgcctgca
     6121 gcaggttggt gataagccgc gtccgaaaac gctgtttgaa ccgggtgaaa tggtccgtgt
     6181 taatgatggt ccgttcgctg acttcaacgg tgttgttgaa gaagtggatt acgagaaatc
     6241 tcgtctgaaa gtgtctgttt ctatcttcgg tcgtgcgacc ccggtagagc tggacttcag
     6301 ccaggttgaa aaagcctaac ccagcgatca aaaaagcggc gatttaatcg ttgcacaagg
     6361 cgtgagattg gaatacaatt tcgcgccttt tgtttttatg ggccttgccc gtaaaacgat
     6421 tttttatatc acggggagcc tctcagaggc gttattaccc aacttgagga atttataatg
     6481 gctaagaaag tacaagccta tgtcaagctg caggttgcag ctggtatggc taacccgagt
     6541 ccgccagtag gtccggctct gggtcagcag ggcgtaaaca tcatggaatt ctgcaaagcg
     6601 ttcaacgcaa aaactgattc catcgaaaaa ggtctgccga ttccggtagt aatcaccgtt
     6661 tacgctgacc gttctttcac tttcgttacc aagaccccgc cggcagcagt tctgctgaaa
     6721 aaagcggctg gtatcaagtc tggttccggt aagccgaaca aagacaaagt gggtaaaatt
     6781 tcccgcgctc agctgcagga aatcgcgcag accaaagctg ccgacatgac tggtgccgac
     6841 attgaagcga tgactcgctc catcgaaggt actgcacgtt ccatgggcct ggtagtggag
     6901 gactaagaaa tggctaaact gaccaagcgc atgcgtgtta tccgcgagaa agttgatgca
     6961 accaaacagt acgacatcaa cgaagctatc gcactgctga aagagctggc gactgctaaa
     7021 ttcgtagaaa gcgtggacgt agctgttaac ctcggcatcg atgctcgtaa atctgaccag
     7081 aacgtacgtg gtgcaactgt actgccgcac ggtactggcc gttccgttcg cgtagccgta
     7141 tttacccaag gtgcaaacgc tgaagctgct aaagctgcag gcgcagaact ggtaggtatg
     7201 gaagatctgg ctgaccagat caagaaaggc gaaatgaact ttgacgttgt tattgcttct
     7261 ccggatgcaa tgcgcgttgt tggccagctg ggccaggttc tgggtccgcg cggcctgatg
     7321 ccaaacccga aagtgggtac tgtaacaccg aacgttgctg aagcggttaa aaacgctaaa
     7381 gctggccagg ttcgttaccg taacgacaaa aacggcatca tccacaccac catcggtaaa
     7441 gtggactttg acgctgacaa actgaaagaa aacctggaag ctctgctggt tgcgctgaaa
     7501 aaagcaaaac cgactcaggc gaaaggcgtg tacatcaaga aagttagcat ctccaccacc
     7561 atgggtgcag gtgttgcagt tgaccaggct ggcctgagcg cttctgtaaa ctaatgcctt
     7621 tacgtgggcg gtgattttgt ctacaatctt acccccacgt ataatgctta atgcagacgt
     7681 atatccgaga tattcgggtt gtggcaaggc ggcaactgag tgagtcgcca ggagcatagc
     7741 taactatgtg actggtgcga atgaaggaag ccaacgccgc cacaagttga atagcgacgg
     7801 atagaaaaga tttgttcgtt ggagcctggc ctatccaggc ctccgtcgaa gaccgcagga
     7861 gtttcgcaag aaacttaatc ccctgcgtag acggtgacag aacgctaaga ttattctttt
     7921 atattctggc ttgtttctgc tcaccgtaat taagacgctc tctccgtttg gaggagtgaa
     7981 gtgagttcca gagattttct ctggcaaaca tccaggagca aagctaatgg ctttaaatct
     8041 tcaagacaaa caagcgattg ttgctgaagt cagcgaagta gccaaaggcg cgctgtctgc
     8101 agtagttgcg gattcccgtg gcgtaactgt agataaaatg actgaactgc gtaaagcagg
     8161 tcgcgaagct ggcgtataca tgcgtgttgt tcgtaacacc ctgctgcgcc gtgctgttga
     8221 aggtactccg ttcgagtgcc tgaaagacgc gtttgttggt ccgaccctga ttgcatactc
     8281 tatggaacac ccgggcgctg ctgctcgtct gttcaaagag ttcgcgaaag cgaatgcaaa
     8341 atttgaggtc aaagccgctg cctttgaagg tgagctgatc ccggcgtctc agatcgaccg
     8401 cctggcaact ctgccgacct acgaagaagc aattgcacgc ctgatggcaa ccatgaaaga
     8461 agcttcggct ggcaaactgg ttcgtactct ggctgctgta cgcgatgcga aagaagctgc
     8521 ttaatcgcag ttatcttttt aacgcattcg cttacgtata aacttattct gatattcagg
     8581 aacaatttaa atgtctatca ctaaagatca aatcattgaa gcagttgcag ctatgtctgt
     8641 aatggacgtt gtagaactga tctctgcaat ggaagaaaaa ttcggtgttt ccgctgctgc
     8701 tgctgtagct gtagctgctg gcccggttga agctgctgaa gaaaaaactg aattcgacgt
     8761 aattctgaaa gctgctggcg ctaacaaagt tgctgttatc aaagcagtac gtggcgcaac
     8821 tggcctgggt ctgaaagaag ctaaagacct ggtagaatct gcaccggctg ctctgaaaga
     8881 aggcgtgagc aaagacgacg cagaagcact gaaaaaagct ctggaagaag ctggcgctga
     8941 agttgaagtt aaataagcca acccttccgg ttgcagcctg agaaatcagg ctgatggctg
     9001 gtgacttttt agtcaccagc ctttttgcgc tgtaaggcgc cagtagcgtt tcacactgtt
     9061 tgactactgc tgtgcctttc aatgcttgtt tctatcgacg acttaatata ctgcgacagg
     9121 acgtccgttc tgtgtaaatc gcaatgaaat ggtttaagcg tgatagcaac aggcattgcg
     9181 gaaagtgttc cattttccgg tcaacaaaat agtgttgcac aaactgtccg ctcaatggac
     9241 agatgggtcg acttgtcagc gagctgagga accctatggt ttactcctat accgagaaaa
     9301 aacgtattcg taaggatttt ggtaaacgtc cacaagttct ggatgtacct tatctccttt
     9361 ctatccagct tgactcgttt cagaaattta tcgagcaaga tcctgaaggg cagtatggtc
     9421 tggaagctgc tttccgttct gtattcccga ttcagagcta cagcggtaat tccgagctgc
     9481 aatacgtcag ctaccgcctt ggcgaaccgg tgtttgacgt ccaggaatgt caaatccgtg
     9541 gcgtgaccta ttccgcaccg ctgcgcgtta aactgcgtct ggtgatctat gagcgcgaag
     9601 cgccggaagg caccgtaaaa gacattaaag aacaagaagt ctacatgggc gaaattccgc
     9661 tcatgacaga caacggtacc tttgttatca acggtactga gcgtgttatc gtttcccagc
     9721 tgcaccgtag tccgggcgtc ttctttgact ccgacaaagg taaaacccac tcttcgggta
     9781 aagtgctgta taacgcgcgc atcatccctt accgtggttc ctggctggac ttcgaattcg
     9841 atccgaagga caacctgttc gtacgtatcg accgtcgccg taaactgcct gcgaccatca
     9901 ttctgcgcgc cctgaactac accacagagc agatcctcga cctgttcttt gaaaaagtta
     9961 tctttgaaat ccgtgataac aagctgcaga tggaactggt gccggaacgc ctgcgtggtg
    10021 aaaccgcatc ttttgacatc gaagctaacg gtaaagtgta cgtagaaaaa ggccgccgta
    10081 tcactgcgcg ccacattcgc cagctggaaa aagacgacgt caaactgatc gaagtcccgg
    10141 ttgagtacat cgcaggtaaa gtggttgcta aagactatat tgatgagtct accggcgagc
    10201 tgatctgcgc agcgaacatg gagctgagcc tggatctgct ggctaagctg agccagtctg
    10261 gtcacaagcg tatcgaaacg ctgttcacca acgatctgga tcacggccca tatatctctg
    10321 aaaccttacg tgtcgaccca actaacgacc gtctgagcgc actggtagaa atctaccgca
    10381 tgatgcgccc tggcgagccg ccgactcgtg aagcagctga aagcctgttc gagaacctgt
    10441 tcttctccga agaccgttat gacttgtctg cggttggtcg tatgaagttc aaccgttctc
    10501 tgctgcgcga agaaatcgaa ggttccggta ttctgagcaa agacgacatc attgatgtta
    10561 tgaaaaagct catcgatatc cgtaacggta aaggcgaagt cgatgatatc gaccacctcg
    10621 gcaaccgtcg tatccgttcc gttggcgaaa tggcggaaaa ccagttccgc gttggcctgg
    10681 tacgtgtaga gcgtgcggtg aaagagcgtc tgtctctggg cgatctggat accctgatgc
    10741 ctcaggatat gatcaacgcc aagccgattt ccgcagcagt gaaagagttc ttcggttcca
    10801 gccagctgtc tcagtttatg gaccagaaca acccgctgtc tgagattacg cacaaacgtc
    10861 gtatctccgc actcggccca ggcggtctga cccgtgaacg tgcaggcttc gaagttcgag
    10921 acgtacaccc gactcactac ggtcgcgtat gtccaatcga aacccctgaa ggtccgaaca
    10981 tcggtctgat caactctctg tccgtgtacg cacagactaa cgaatacggc ttccttgaga
    11041 ctccgtatcg taaagtgacc gacggtgttg taactgacga aattcactac ctgtctgcta
    11101 tcgaagaagg caactacgtt atcgcccagg cgaactccaa cctggatgaa ggaggccact
    11161 tcgtagaaga cctggtaact tgccgtagca aaggcgaatc cagcttgttc agccgcgacc
    11221 aggttgacta catggacgta tccacccagc aggtggtatc cgtcggtgcg tccctgatcc
    11281 cgttcctgga acacgatgac gccaaccgtg cattgatggg tgcgaacatg caacgtcagg
    11341 ccgttccgac tctgcgcgct gataagccgc tggttggtac tggtatggaa cgtgctgttg
    11401 ccgttgactc cggtgtaact gcggtagcta aacgtggtgg tgtcgttcag tacgtggatg
    11461 cttcccgtat cgttatcaaa gttaacgaag acgagatgta tccgggtgaa gcaggtatcg
    11521 acatctacaa cctgaccaaa tacacccgtt ctaaccagaa cacctgtatc aaccagatgc
    11581 cgtgtgtgtc tctgggtgaa ccggttgaac gtggcgacgt gctggcagac ggtccgtcca
    11641 ccgacctcgg tgaactggcg cttggtcaga acatgcgcgt agcgttcatg ccgtggaatg
    11701 gttacaactt cgaagactcc atcctcgtat ccgagcgtgt tgttcaggaa gaccgtttca
    11761 ccaccatcca cattcaggaa ctggcgtgtg tgtcccgtga caccaagctg ggtccggaag
    11821 agatcaccgc tgacatcccg aacgtgggtg aagctgcgct ctccaaactg gatgaatccg
    11881 gtatcgttta cattggtgcg gaagtgaccg gtggcgacat tctggttggt aaggtaacgc
    11941 cgaaaggtga aactcagctg accccagaag aaaaactgct gcgtgcgatc ttcggtgaga
    12001 aagcctctga cgttaaagac tcttctctgc gcgtaccaaa cggtgtatcc ggtacggtta
    12061 tcgacgttca ggtctttact cgcgatggcg tagaaaaaga caaacgtgcg ctggaaatcg
    12121 aagaaatgca gctcaaacag gcgaagaaag acctgtctga agaactgcag atcctcgaag
    12181 cgggtctgtt cagccgtatc cgtgctgtgc tggtagccgg tggcgttgaa gctgagaagc
    12241 tcgacaaact gccgcgcgat cgctggctgg agctgggcct gacagacgaa gagaaacaaa
    12301 atcagctgga acagctggct gagcagtatg acgaactgaa acacgagttc gagaagaaac
    12361 tcgaagcgaa acgccgaaaa atcacccagg gcgacgatct ggcaccgggc gtgctgaaga
    12421 ttgttaaggt atatctggcg gttaaacgcc gtatccagcc tggtgacaag atggcaggtc
    12481 gtcacggtaa caagggtgta atttctaaga tcaacccgat cgaagatatg ccttacgatg
    12541 aaaacggtac gccggtagac atcgtactga acccgctggg cgtaccgtct cgtatgaaca
    12601 tcggtcagat cctcgaaacc cacctgggta tggctgcgaa aggtatcggc gacaagatca
    12661 acgccatgct gaaacagcag caagaagtcg cgaaactgcg cgaattcatc cagcgtgcgt
    12721 acgatctggg cgctgacgtt cgtcagaaag ttgacctgag taccttcagc gatgaagaag
    12781 ttatgcgtct ggctgaaaac ctgcgcaaag gtatgccaat cgcaacgccg gtgttcgacg
    12841 gtgcgaaaga agcagaaatt aaagagctgc tgaaacttgg cgacctgccg acttccggtc
    12901 agatccgcct gtacgatggt cgcactggtg aacagttcga gcgtccggta accgttggtt
    12961 acatgtacat gctgaaactg aaccacctgg tcgacgacaa gatgcacgcg cgttccaccg
    13021 gttcttacag cctggttact cagcagccgc tgggtggtaa ggcacagttc ggtggtcagc
    13081 gtttcgggga gatggaagtg tgggcgctgg aagcatacgg cgcagcatac accctgcagg
    13141 aaatgctcac tgttaagtct gatgacgtga acggtcgtac caagatgtat aaaaacatcg
    13201 tggacggcaa ccatcagatg gagccgggca tgccagaatc cttcaacgta ttgttgaaag
    13261 agattcgttc gctgggtatc aacatcgaac tggaagacga gtaattctcg ctcaaacagg
    13321 tcactgctgt cggggtaaaa cccggcagcg gattgtgcta actccgacgg gagcaaatcc
    13381 gtgaaagatt tattaaagtt tctgaaagcg cagactaaaa ccgaagagtt tgatgcgatc
    13441 aaaattgctc tggcttcgcc agacatgatc cgttcatggt ctttcggtga agttaaaaag
    13501 ccggaaacca tcaactaccg tacgttcaaa ccagaacgtg acggcctttt ctgcgcccgt
    13561 atctttgggc cggtaaaaga ttacgagtgc ctgtgcggta agtacaagcg cctgaaacac
    13621 cgtggcgtca tctgtgagaa gtgcggcgtt gaagtgaccc agactaaagt acgccgtgag
    13681 cgtatgggcc acatcgaact ggcttccccg actgcgcaca tctggttcct gaaatcgctg
    13741 ccgtcccgta tcggtctgct gctcgatatg ccgctgcgcg atatcgaacg cgtactgtac
    13801 tttgaatcct atgtggttat cgaaggcggt atgaccaacc tggaacgtca gcagatcctg
    13861 actgaagagc agtatctgga cgcgctggaa gagttcggtg acgaattcga cgcgaagatg
    13921 ggtgcggaag caatccaggc tctgctgaag agcatggatc tggagcaaga gtgcgaacag
    13981 ctgcgtgaag agctgaacga aaccaactcc gaaactaagc gtaaaaagct gaccaagcgt
    14041 atcaaactgc tggaagcgtt cgttcagtct ggtaacaaac cagagtggat gatcctgacc
    14101 gttctgccgg tactgccgcc agatctgcgt ccgctggttc cgctggatgg tggtcgtttc
    14161 gcgacttctg acctgaacga tctgtatcgt cgcgtcatta accgtaacaa ccgtctgaaa
    14221 cgtctgctgg atctggctgc gccggacatc atcgtacgta acgaaaaacg tatgctgcag
    14281 gaagcggtag acgccctgct ggataacggt cgtcgcggtc gtgcgatcac cggttctaac
    14341 aagcgtcctc tgaaatcttt ggccgacatg atcaaaggta aacagggtcg tttccgtcag
    14401 aacctgctcg gtaagcgtgt tgactactcc ggtcgttctg taatcaccgt aggtccatac
    14461 ctgcgtctgc atcagtgcgg tctgccgaag aaaatggcgc tggagctgtt caaaccgttc
    14521 atctacggca agctggaact gcgtggtctt gctaccacca ttaaagctgc gaagaaaatg
    14581 gttgagcgcg aagaagctgt cgtttgggat atcctggacg aagttatccg cgaacacccg
    14641 gtactgctga accgtgcacc gactctgcac cgtctgggta tccaggcatt tgaaccggta
    14701 ctgatcgaag gtaaagctat ccagctgcac ccgctggttt gtgcggcata taacgccgac
    14761 ttcgatggtg accagatggc tgttcacgta ccgctgacgc tggaagccca gctggaagcg
    14821 cgtgcgctga tgatgtctac caacaacatc ctgtccccgg cgaacggcga accaatcatc
    14881 gttccgtctc aggacgttgt actgggtctg tactacatga cccgtgactg tgttaacgcc
    14941 aaaggcgaag gcatggtgct gactggcccg aaagaagcag aacgtctgta tcgctctggt
    15001 ctggcttctc tgcatgcgcg cgttaaagtg cgtatcaccg agtatgaaaa agatgctaac
    15061 ggtgaattag tagcgaaaac cagcctgaaa gacacgactg ttggccgtgc cattctgtgg
    15121 atgattgtac cgaaaggtct gccttactcc atcgtcaacc aggcgctggg taaaaaagca
    15181 atctccaaaa tgctgaacac ctgctaccgc attctcggtc tgaaaccgac cgttattttt
    15241 gcggaccaga tcatgtacac cggcttcgcc tatgcagcgc gttctggtgc atctgttggc
    15301 atcgatgaca tggtcatccc ggagaagaaa cacgaaatca tctccgaggc agaagcagaa
    15361 gttgctgaaa ttcaggagca gttccagtct ggtctggtaa ctgcgggcga acgctacaac
    15421 aaagttatcg atatctgggc tgcggcgaac gatcgtgtat ccaaagcgat gatggataac
    15481 ctgcaaactg aaaccgttat taaccgtgac ggtcaggaag agaagcaggt ttccttcaac
    15541 agcatctaca tgatggccga ctccggtgcg cgtggttctg cggcacagat tcgtcagctt
    15601 gctggtatgc gtggtctgat ggcaaagccg gatggctcca tcatcgaaac gccaatcacc
    15661 gcgaacttcc gtgaaggtct gaacgtactc cagtacttca tctccaccca cggtgctcgt
    15721 aaaggtctgg cggataccgc actgaaaact gcgaactccg gttacctgac tcgtcgtctg
    15781 gttgacgtgg cgcaggacct ggtggttacc gaagacgatt gtggtaccca tgaaggtatc
    15841 atgatgactc cggttatcga gggtggtgac gttaaagagc cgctgcgcga tcgcgtactg
    15901 ggtcgtgtaa ctgctgaaga cgttctgaag ccgggtactg ctgatatcct cgttccgcgc
    15961 aacacgctgc tgcacgaaca gtggtgtgac ctgctggaag agaactctgt cgacgcggta
    16021 aaagtacgtt ctgttgtatc ttgtgacacc gactttggtg tatgtgcgca ctgctacggt
    16081 cgtgacctgg cgcgtggcca catcatcaac aaaggtgaag caatcggtgt tatcgcggca
    16141 cagtccatcg gtgaaccggg tacacagctg accatgcgta cgttccacat cggtggtgcg
    16201 gcatctcgtg cggctgctga atccagcatc caggtgaaaa acaaaggtag catcaagctc
    16261 agcaacgtga agtcggttgt gaactccagc ggtaaactgg ttatcacttc ccgtaacacc
    16321 gaactgaaac tgatcgacga attcggtcgt accaaagaaa gctacaaagt gccttacggt
    16381 gcggtactgg cgaaaggcga tggcgaacag gttgctggcg gcgaaaccgt tgcaaactgg
    16441 gacccgcaca ccatgccggt tatcaccgaa gtaagcggtt ttgtacgctt tactgacatg
    16501 atcgacggcc agaccattac tcgtcagacc gacgaactga ccggtctgtc ttcgctggtg
    16561 gttctggatt ccgcagaacg taccgcaggt ggtaaagatc tgcgtccggc actgaaaatc
    16621 gttgatgctc agggtaacga cgttctgatc ccaggtaccg atatgcctgc gcagtacttc
    16681 ctgccgggta aagcgattgt tcagctggaa gatggcgtac agatcagctc tggtgacacc
    16741 ttggcgcgta ttccgcagga atccggcggt accaaggaca tcaccggtgg tctgccgcgc
    16801 gttgcggacc tgttcgaagc tcgtcgtccg aaagaaccgg caatcctggc tgaaatcagc
    16861 ggtatcgttt ccttcggtaa agaaaccaaa ggtaaacgtc gtctggttat caccccggta
    16921 gacggtagcg atccgtacga agagatgatt ccgaaatggc gtcagctcaa cgtgttcgaa
    16981 ggtgaacgtg tagaacgtgg tgacgtaatt tccgacggtc cggaagcgcc gcacgacatt
    17041 ctgcgtctgc gtggtgttca tgctgtgact cgttacatcg ttaacgaagt acaggacgta
    17101 taccgtctgc agggcgtaaa gattaacgat aaacacatcg aagttatcgt tcgtcagatg
    17161 ctgcgtaaag ctaccatcgt taacgcgggc agctccgact tcctggaagg cgaacaggtt
    17221 gaatactctc gcgtcaagat cgcaaaccgc gaactggaag cgaacggcaa agtgggtgca
    17281 acttactccc gcgatctgct gggtatcacc aaagcgtctc tggcaaccga gtccttcatc
    17341 tccgcggcat cgttccagga gaccactcgc gtgctgaccg aagcagccgt tgcgggcaaa
    17401 cgcgacgaac tgcgcggcct gaaagagaac gttatcgtgg gtcgtctgat cccggcaggt
    17461 accggttacg cgtaccacca ggatcgtatg cgtcgccgtg ctgcgggtga agctccggct
    17521 gcaccgcagg tgactgcaga agacgcatct gccagcctgg cagaactgct gaacgcaggt
    17581 ctgggcggtt ctgataacga gtaatcgtta atccgcaaat aacgtaaaaa cccgcttcgg
    17641 cgggtttttt tatgggtgtt attcagaggg caaattcttc acggaaaata ttaagaaagg
    17701 cagggcgaaa cgaggaagaa gcttttcgcc ctggacaaca ttcctgctga cggcactacc
    17761 ataaaaaaac acaacgcttt caacaacctt gcgtttttca tcttccgtag cggctcggaa
    17821 taaatttacg ttcttacatt ccgtctcata gtctttgcga ggcgcgtccc agatagctgt
    17881 cccagtcttt ccataccggc tgcaaaccct gagcggttaa cgcggcagca accgcttccg
    17941 gtctgcgatc gtcgtgcggt gagaactgtt ccaactcggg gtgattatcg gcatagccac
    18001 ctggctgcgt tttcgagaag gcgctgacgt tattgatcgc cagcggaata acgcgatcgc
    18061 gaaaccacgg tgattcccgc gtggagagtg acagttcaat ctccggtgca agcagtcgga
    18121 aggcgcagat ggtttgcact aactggcgtt catccataat cgacgcaggc tcaatgccgc
    18181 cagtacacgg gcgcaggcgc ggaaaggaga cagagtaacg gctttgccag taatgctgtt
    18241 gcagccatag caaatgttcg gcaaccatat agcagtcaac gcgccagttg tcggaaaggc
    18301 caattagcgc gccgaggcct atcttatcaa tccccgcacg ccccagccga tccggcgttt
    18361 ccagccgcca gaagaagtcc tgttttttgc ctttcaaatg atggcgggta taagtcgcct
    18421 cgtgatatgt ctcctgataa accatcaccc catccagacc aagttgcttt aactcggcgt
    18481 attccgtctc cgccagcggt tgcacttcca tttgtagtga agagaactgt tcacgcaggg
    18541 cggggagatg acgacgaaag taatccatcc ccactttcgc ctgatgttca ccagtgacta
    18601 atagcagatg ttcaaagccc atctcccgta tagcggcgct ttccctggca atatccgctt
    18661 catccagcgt tttgcgcttg atgcgattac tcatggaaaa tccgcagtac gtgcagttgt
    18721 tagcgcaaag attggaaaga taaagcggga cgtagaaact aactgtgttg ccaaatcgct
    18781 gacgggtcag acgctgcgcc tgttgggcca gttgttccag atagccactg gcggcaggcg
    18841 ataacagcgc catcatgtcg tcgcgggtga gttgcgaggc atttagcgcc cgctctacgt
    18901 cagcagccgt tttgccgttg atacgcaggc ggatgtcgtc ccagtccagt tgtcgccagc
    18961 gattgctgaa ggttttcatg ccgatgcctc cagaaatccg gtcagcgggc tggtggcatg
    19021 agcaaaataa ctgcggctgc ccggtccgga ctgacgtgcc agcaggcctg cttctaccgc
    19081 cagacgaaat gccttcgcca tgttgacggg atcgtccgcg acggcaatcg ccgtattcac
    19141 taacaccgcg tcggccccca tttccagcgc ctgcgcggca tggctgggaa cgccgatgcc
    19201 agcatcgaca accaccggaa ctgtggcctg ctggataata atctccagca tggcacgggt
    19261 ttccagtccc tgattcgagc caatcggcgc gccgagcggc atcaccgctg cacagccgac
    19321 ttcttccaga cgtttacaca ataccggatc ggccccgcag taaggcagca cgacaaatcc
    19381 ctgttgtacc agcgtttcgg cggctttcag ggtttcgatg ggatcgggca acagccagcg
    19441 ggcgtcaggg tgaatctcca attttagcca gtttgtgcct aacgcttcac gtgccagatg
    19501 ggcggcgaaa atggcttctt ccgctgtttt cgccccggaa gtattcggca gcagggccac
    19561 acccgccgcg ataagcggtt cgaggatagc gtcgttgtgc tggcgcaagt cgacacgttt
    19621 catcgccagt gtcaccagct ggctgccgga agcgcggatc gcctccacca tcagttgtga
    19681 agaagcgaat ttccctgtgc cggtaaacag atgtgaatca aacgttttgt ccacaatacg
    19741 taacatctca accccctgca ataacctgaa aaagcaggat ctggtcgcca tcctgcacga
    19801 tatgttgcgt ccactgctca cgcgggacga tttgctgatt aatcgccaga gccgcgcccg
    19861 cttgtcgttg gtcgagttgc tccagtagtt cgtgaacagt ttgcccggcg gcgcactgca
    19921 tcggttgatc gttaaacagg atctgcattg cttcctccgc ataccgggca accactggcg
    19981 cggcgcaacg ccaggctgcg ccactggctc gacttaccgt cgaacagtcg gagttctccc
    20041 gcaggcgtct ctataccgct taataactta atggcttcca gtgcctgcaa agtgcccatc
    20101 accccgacca ccgggccaac cacgcccgcc gtgcggcagt tgcgttctgg ctgctggtta
    20161 tctggccaca ggcagcggta acacccctgc tcccagggcg gtgtcagtac catcaactga
    20221 ccgccaaatc cgaccgcgct ggcggtgata agcggcgtgt tgagtgccac gcaggcggca
    20281 ttaatctcct ggcgagtcgc catattgtcg gtacagtcga gcaccacatc ggcttgtgca
    20341 accgcatctt tcaacgcctc acccgttaac cgttgttgta atgctgtcag ttgaatgtcg
    20401 ggattcaact gtgtcagtcg ctgttggctg acctgcgatt tcgggctgtt gatatcttca
    20461 gtggtaaaga ggatttgtcg ttgcagattg cttaaatgca catcgtcgtc atctgccagt
    20521 accagcgtcc cgacgccagc ccctgccagg tatagcgcag caggtgtacc cagcccgcca
    20581 agaccaataa tcagcacctg gctgtcgagc agtttttgct gcccgtcaag agcgatatcg
    20641 tcgagcagaa tttggcggct ataacgcata aagtcacggt cattcatcgc caactcctgc
    20701 aatttccagc aactgtgccg ttgccaaacg ccagtctgcg gcttgagtaa tggcgctgac
    20761 gacggcgata ctgccgacac ccgttgctat caccgcaggc gcgcgtgcca gactgatacc
    20821 gccaatcgcc acggtgggat aatccgccag gcgctcaaca tgccgtgcca actgttccag
    20881 cccctgcggt gcagaaggca tctgtttggt ttgcgtcggg aacacatgtc ccagcgcgat
    20941 ataagagggg cgtgctgcca gcgcgacgtc gatttccata tcgtcatgtg tcgaaacgcc
    21001 cagccgcagg cctgccgcgc ggatggcatt gagatcggtg gcttgcaaat cttcctgccc
    21061 caaatggacg ccatacgcct gatgcttgat cgcgagccgc cagtaatcgt tgataaacaa
    21121 tcgcgcgtta tagcggcggc ccagcgcaat tgccgccacg acatcggctt ccacctcttc
    21181 atcgcgccga tctttgatgc gtagctggag agtacgtacg cctgcatcca acagacgttc
    21241 gatccactgt acgctgtcca ccaccgggta cagtcctaaa cgaaaaggta caggaggaaa
    21301 atcaggctga tacatcacgc ttcctcctta tgcaggtaga tttctccgcc tctggcacgg
    21361 aagttctccg acatatccgc cattcccact tcaatggttt gcgcggcggc gtaatcgcgt
    21421 acttcctggc tgattttcat cgagcagaat tttggcccac acatggagca aaaatgggcg
    21481 actttgccgg actcttgggg cagggtttca tcgtgataag cgcgggcggt aaacgggtcg
    21541 agggccagat taaactggtc ttcccagcga aattcgaagc gggctttcga catggcgtta
    21601 tcgcgaattt gcgcgcccgg atgccctttt gccaggtcag cagcgtgggc ggcaatctta
    21661 taagtgataa gcccctgctt aacatcttct ttattgggca gacccagatg ctcttttggc
    21721 gttacgtaac agagcatcgc gcagccaaac cagccaatca tcgccgcacc aatccccgac
    21781 gtgaagtggt catagcccgg tgcaatatcg gtagttagcg gccccagagt gtaaaacggc
    21841 gcttcgtggc agtgctctaa ctcctcggtc atattgcggc ggatcatctg catcggcaca
    21901 tggcctgggc cttcaatcat cacctgtacg tcatattccc aggcgatttt ggtcagttcg
    21961 cccagcgtat gcaattcggc aaactgcgct tcatcgttgg cgtcctgaat agaaccgggg
    22021 cgcagaccgt cgcccagcga cagcgaaacg tcataagcgg cacagatttc acaaatttcg
    22081 cggaagtgtt gatagaggaa attttcctga tgatgggaga ggcaccattt cgccataatc
    22141 gaaccgccgc gagagacgat accggtcagg cgtttcgcgg tcatcggcac atagcgcagc
    22201 agtacgcccg catggatagt gaagtaatcc acaccttgct cggcctgttc cagcagcgtg
    22261 tcgcggaacg cttcccaggt aagatcttcg gcgatcccgt taaccttctc cagcgcctgg
    22321 tagatcggca ctgtaccgat cggcaccgga ctgttacgca aaatccactc gcgggtttcg
    22381 tgaatatagc gaccggtgga gagatccatc accgtatccg ctccccagcg cgtggaccat
    22441 accagctttt ccacttcttc ttcgatggaa gaggtgaccg ccgagttgcc gatattggcg
    22501 ttaactttta ccaggaagtt acggccaata atcatcggct ccgattccgg atgattaatg
    22561 ttggccggga taatcgcacg tccggcagca acttcatcac ggacaaattc cgcagtgata
    22621 ttttccggca gatgtgcgcc aaagctcatt cccggatgct ggtggcgtaa aacctcgcta
    22681 cggatgcgct cgcggcccat attctcgcgg atggcgatga attccatttc cggcgtgatg
    22741 atgccctggc gggcgtagtg cagttgggtg acacggcgtc ctgttttggc gcgttttggc
    22801 gttaataagc cgctaaaacg tagctcgtcg agaccatcat ctgccagccg cgttctggtg
    22861 taatcggaac tgcgcacggt aagttcttcg gtatcggcgc gcgcatcgat ccacggctgg
    22921 cgtagttttg ccagcccttg ctgcacgtta atggcgatct gcggatcacc atacgggccg
    22981 gaggtgtcgt agaccggaat cgcttcgttt tcttcgtact gcggctgttc tttgctaccg
    23041 ccaattagcg tcgggctaag ctggatctca cgcatcggca cgcgcacgcc ggggtgtgtg
    23101 ccagtgatat aaatgcgttt tgagttggga aaggcggtgc cttccagggt gtcgataaaa
    23161 tgttgggccc gggcgcgttg ttcgcggcgg gtcagttttg ttgcagacat agctcattcc
    23221 aaaaagttaa ggacgtggct tgtcagacga cggatgaagc aagagacgat cgccgcaaag
    23281 gcgatgcgat agcagattaa ctcttgttcc cttcgcaggt attagcctga tcaggttccg
    23341 cggatcccga ataaacggtc tcagccagtt aaggcactcc gacaagaaat tagcccccac
    23401 aaacggggcg ttgaatgtaa attacgcgtt aacagcgcag aactcaagca ggatgtttga
    23461 cgcgggcggc gtcaagcacc agcagaatca gcttatcttc cagcacaaag cgcgcttcca
    23521 gcgcctcgcc gatgtcagat aaaacctgtt gaaactcaag gtaattatca tgatcgatgg
    23581 cggtttccag gctggaatcg tagtaatcca taatctgttg ggtattggct tcgagttgcg
    23641 gccaaatctt cgcggcgcgc gcgagttgcc cgttgccttc cagcttatga agaatgcgct
    23701 cataaatact gaaatgtccg gcagacaagt aatcgaccag gctctgacaa aaatcatcaa
    23761 gggctttttc gtttagcctc atgtacgatt ctttgccagg cttaatgcca accagattgt
    23821 agtaagccac gagcagatgc ttacgtacat gtagccagcg atcaaccagt ttgttacttc
    23881 ctctgacgcg ctccgtcagg ttatcgagct ggttaagcat gattgactcc gcaagtttgt
    23941 attcaaaaac tgctcagtga gaaatgtaaa aaccatgtta aacatgccag tgatgcaaag
    24001 gtagtgcaag agctatggat cgtataattg aaaaattaga tcacggctgg tgggtcgtca
    24061 gccatgaaca aaaattatgg ttgccgaagg gagaattgcc atatggcgaa gcggcaaatt
    24121 tcgatcttgt gggtcagcgc gcactacaaa tcggcgaatg gcagggggaa cctgtttggt
    24181 taatacaaca gcagcggcgt cacgatatgg ggtcggtacg tcaggtcatt gatctcgatg
    24241 ttgggctgtt tcaactggcc ggacgtggcg tgcaactggc ggagttttac cgatcgcata
    24301 aatactgtgg ttactgcggg catgaaatgt atccgagcaa aaccgaatgg gcgatgctgt
    24361 gcagccattg ccgtgagcgt tactacccgc aaatcgcccc ctgcattatt gttgccatcc
    24421 gtcgcgatga ttcgatcctc ctcgcccagc atacccgcca tcgtaacggt gtccatacag
    24481 tacttgccgg attcgtcgaa gtgggcgaaa ccctcgagca ggcagtcgcg cgggaagtga
    24541 tggaagagag cggaattaaa gttaaaaact tgcgttacgt gacttctcag ccgtggccgt
    24601 ttcctcagtc tttaatgacc gcgtttatgg cggaatatga cagcggcgac atcgtgatcg
    24661 acccgaaaga attgctcgag gcgaactggt atcgctatga cgatttgccg ttactcccgc
    24721 cgcccggcac cgtagcgcgc cgtctgatag aagatactgt ggcgatgtgt cgggcagagt
    24781 atgagtgatg atacactgac cgcctgacgc actaaggaac agccaaaatg accgaactta
    24841 aaaacgatcg ttatctgcgg gcgctgctgc gccagcccgt tgatgtcact ccagtatgga
    24901 tgatgcgcca ggcgggtcgc tatctaccgg aatataaagc cacgcgcgcc caggcgggcg
    24961 attttatgtc gctgtgcaaa aacgccgagc tggcgtgcga agtgactttg caaccgctgc
    25021 gtcgctaccc gctggatgcg gcgatcctct tttccgatat cctcaccgtg ccggacgcga
    25081 tggggttagg gctctatttt gaagccggag aaggtccgcg ttttacctcg ccagtcacct
    25141 gcaaagccga cgtcgataaa ctgccaattc cggacccgga agatgagctg ggttacgtga
    25201 tgaacgcggt gcgtaccatt cgtcacgaac tgaaaggcga agtgccgctg attggttttt
    25261 ccggcagccc gtggacgctg gcaacctaca tggtggaagg cggcagcagc aaagccttca
    25321 ccgtgatcaa aaaaatgatg tatgccgatc cgcaggcgct gcacgctctg ctcgataaac
    25381 tggcgaaaag cgtcactttg tatctgaatg cgcagattaa agccggtgct caggcagtga
    25441 tgattttcga cacctggggc ggcgtgctta ccgggcgcga ttatcaacag ttctcgctct
    25501 attacatgca taaaattgtt gatggtttac tgcgtgaaaa cgacggtcgc cgcgtgccag
    25561 tcacgctgtt taccaaaggc ggcggacagt ggctggaagc catggctgaa accggttgcg
    25621 atgcgctggg cctcgactgg acaacggaca tcgccgatgc gcgccgccgt gtgggcaata
    25681 aagtcgcgtt gcagggtaat atggatccgt cgatgctgta cgcgccgcct gcccgcattg
    25741 aagaagaagt agcgactata cttgcaggtt tcggtcacgg cgaaggtcat gtctttaacc
    25801 ttggtcacgg cattcatcag gatgtgccgc cagaacatgc tggcgtgttc gtggaggcag
    25861 tgcatcgact gtctgaacag tatcaccgct aagagcctga tccatcaggc tattttattt
    25921 gccattttga ggccgggcag tgctcaaaat cctcacgtac tacgtgtacg ctccggtttt
    25981 tccgcgctgt ccggcctcaa actgccttca acaattacgc ctgttgaacc aggctctaag
    26041 gagcgattat ggatctcgcg tcattacgcg ctcaacaaat cgaactggct tcttctgtga
    26101 tctgcgagga tcgactcgat aaagatccac cggatctgat cgccggagcc gatgtcgggt
    26161 ttgagcaggg cggagaagtg acgcgagcgg cgatggtgct gctgaaatat ccctcgcttg
    26221 agctggtgga gtataaagtt gcccgcatcg ccaccaccat gccttacatt ccaggttttc
    26281 tttccttccg cgaatatcct gcgctgctgg cagcgtggga gatgctgtcg caaaagccgg
    26341 atttagtgtt tgtcgatggt catgggatct cgcatcctcg ccgtcttggc gtcgccagcc
    26401 attttggctt attggtggat gtgccgacca ttggcgtggc gaaaaaacgg ctctgcggta
    26461 aattcgaacc gctctccagc aaaccgggcg cgctggcccc actgatggat aaaggcgagc
    26521 agctggcctg ggtctggcgc agcaaagcgc gctgtaaccc gttgtttatc gctaccggcc
    26581 atcgggtcag cgtggacagc gcgctggcgt gggtacaacg ctgcatgaaa ggctatcgtc
    26641 tgccggagcc aacgcgctgg gcggacgcgg tggcctcgga acgtccggcg ttcgtgcgct
    26701 atacagcaaa tcagccctaa ttcaggtaaa ctgcggataa tttccgtatt tgagaactca
    26761 acatgttaca aaacccaatt catctgcgtc tggagcgcct ggaaagctgg cagcatgtca
    26821 ctttcatggc ttgcttatgc gaacgcatgt accccaatta cgccatgttc tgccagcaaa
    26881 ccggttttgg tgatgggcaa atttaccgtc gtattctcga tctcatctgg gaaacgctga
    26941 ccgttaaaga cgcaaaagta aatttcgaca gccaactgga gaaatttgaa gaagcgattc
    27001 cttcagccga cgatttcgat ctgtacggcg tttatccggc aatcgatgcc tgcgtggcgt
    27061 taagtgaact ggtccattcg cgtttgagtg gtgaaacgct cgaacacgcg gtggaagtga
    27121 gtaagacctc catcacgacc gttgcgatgc tggaaatgac tcaggctggt cgcgaaatga
    27181 gcgatgaaga gcttaaagaa aacccagctg tagagcaaga atgggacatt cagtgggaaa
    27241 tattccgact tttagctgag tgcgaagaac gcgatatcga gctgataaaa ggccttaggg
    27301 cagacctgcg tgagtcgggt gagagcaata ttggtataat ttttcagcaa taagaccaga
    27361 aaacgtgatt taacgcctga tttgtcgtac ctggagtctt ccctttcgcc ccccgtctgg
    27421 tctacatttg gggggcgaaa aaaagtggct atcggtgcgt gtatgcagga gagtgctatt
    27481 ctggcatttc cgtcgcactc gatgcttagc aagcgataaa cacattgtaa ggataactta
    27541 tgaacaagac tcaactgatt gatgtaattg cagagaaagc agaactgtcc aaaacccagg
    27601 ctaaagctgc tctggagtcc actctggctg caattactga gtctctgaaa gaaggcgatg
    27661 ctgtacaact ggttggtttc ggtaccttca aagtgaacca tcgcgctgag cgtactggcc
    27721 gcaacccgca gaccggtaaa gaaatcaaaa ttgccgcagc taacgtaccg gcatttgttt
    27781 ctggcaaggc actgaaagac gcagttaagt aagattgcgt ggcagtgaac agttttaacg
    27841 aaggggtggt ttcacccctt ttgtctttct ggcgtcgatc attgatgctg gcaggtgcgc
    27901 tgtttctcac tgcctgtagc cacaactctt cacttcctcc ctttaccgcc agtggatttg
    27961 ctgaagacca gggcgcggta cgcatctggc gaaaagacag cggcgataat gtgcatctgc
    28021 ttgccgtgtt tagcccgtgg cgcagtggcg ataccacgac gcgagagtat cgctggcagg
    28081 gcgataacct cacgctcatc aatatcaatg tttacagcaa accgccggtg aacattcgcg
    28141 cgcgttttga cgatcgcggc gatctgagct ttatgcaacg tgaatccgat ggggaaaagc
    28201 agcagctttc taacgaccaa atcgatttat accgttatcg tgctgatcag atccgccaga
    28261 ttagcgatgc cttacgtcag gggagagtcg tgctgcgcca ggggcgctgg catgcgatgg
    28321 aacagaccgt gaccacctgc gaagggcaaa ccattaaacc tgatttagat tcgcaggcga
    28381 tagcgcatat cgagcgccgc cagagccgct cttctgttga tgtcagcgtg gcatggctgg
    28441 aagcgcccga aggttcgcaa ttactgttag tggcaaactc tgatttctgt cgctggcaac
    28501 ccaacgagaa aacgttctga tttaccagtg gcccataccc atatgaccgc caccaccgca
    28561 gccgccgtag cccatgccca ttccggctcc gcgcggaata cccgcttcag ccatcgcgat
    28621 atctcgtttc acccgtaact catctaacga ctgacgcaaa ttctccatct ctttggcgac
    28681 cgcgttaatt ttgctgctat ccggtgggtt cgcggctaac agggcattgt attcataacg
    28741 cttcgtcacc agttgctgtt gcagtgcgct gctttgagcg taaaagtcat tatggatttt
    28801 ctgccacgct gtctgttgtt cgctggtcaa aggcgcggca ttttgctgcc acataccgtg
    28861 tccgccgtga gcaaatgcag atgtcgatcc catcgccatt gctgaaagcg ccatcattac
    28921 cagggcaatt ttcgtgttcc gtttcatggt taatcctcca gtggttgtct tacttcgggt
    28981 attgcatctt ccgtgccaac gatgaaacgc tgatatgacg ggtaatctgg catgataaac
    29041 gagtaaaaat gactcgcctg ctgcgggtag cgagtcattt ttactcattg aaacttaagc
    29101 ctttgtgtta cagcgcaggg taagcgctga taaaagatgg catgatttct gctgtcagaa
    29161 agggatgagc aggcaaagaa gaagatgcgt tttatgcaac gttctaaaga ctccttagct
    29221 aaatggttaa gcgcgatcct ccccgtggtc attgttgggc tggtagggtt gtttgcggtg
    29281 acggtgattc gcgattatgg gcgcgagact gccgccgcca gacaaacgct gctggaaaaa
    29341 ggcagtgtac ttattcgcgc tcttgaatcc ggctcgcgcg tcggcatggg gatgcgcatg
    29401 catcatgcgc agcagcaggc cttactggaa gaaatggccg ggcagcctgg agtacgttgg
    29461 tttgcggtca cggatgaaca aggaacaatc gtgatgcata gcaactccgg catggtggga
    29521 aaacagcttt attcccctca ggaaatgcag cagttacatc cgggagatga agaagcgtgg
    29581 cggcggatcg atagcgcaga cggcgagcct gttctggaaa tttatcgcca gtttcaaccg
    29641 atgtttgctg ctggaatgca ccggatgcgc catatgcagc agtatgccgc gacaccacaa
    29701 gcaattttca tcgctttcga cgccagtaat attgtgagtg ccgaagatcg tgagcagaga
    29761 aacaccctga ttattctctt cgccctggcg acggtcttgc tggcaagcgt attgtcattc
    29821 ttctggtatc gccgctatct gcgctcgcgc cagcttctac aagatgaaat gaagcgcaaa
    29881 gagaagctgg tggcgctagg gcatcttgcg gcaggcgttg cccacgaaat ccgtaaccca
    29941 ctttcctcga ttaaaggact ggcgaaatac tttgccgagc gcgcgcctgc agggggagaa
    30001 gcgcatcaac tggcgcaggt gatggcgaaa gaggccgacc gtttaaaccg cgtggtaagc
    30061 gagttgctgg aactggttaa gccaacgcat ctggctttgc aggcggtgga tctcaacacg
    30121 ctgattaacc actcattaca gctggtaagc caggatgcaa acagccggga gatccagtta
    30181 cgctttaccg ccaacgacac attaccggaa attcaggccg atccggacag gctgactcag
    30241 gtcctgttga atctctatct caatgctatt caggcgattg gtcagcatgg cgtgattagc
    30301 gtgacggcca gcgaaagcgg cgcgggcgtg aaaatcagcg ttaccgacag cggtaaggga
    30361 attgcggcag atcagcttga agccatcttc actccgtact tcaccaccaa agccgaaggc
    30421 accggattgg ggctggcggt cgtgcataat attgttgaac aacacggtgg tacaattcag
    30481 gtcgcaagcc aggagggaaa aggctcaacg ttcaccctct ggcttccggt caatattacg
    30541 cgtaaggacc cacaaggatg acgcacgata atatcgatat tctggtggtg gatgatgaca
    30601 ttagccactg cactattttg caggctttac tgcgcggctg gggctataac gtcgcgctgg
    30661 cgaacagcgg gcgacaggcg ctggagcagg tgcgggaaca ggtttttgat cttgtgcttt
    30721 gcgatgtgcg aatggcggag atggacggca tcgccacgct gaaagagatc aaagcgttaa
    30781 acccggcaat tccggtgctg attatgactg cgtactccag cgtcgagacg gcggtagagg
    30841 cactgaaaac tggggcgctg gattatctca tcaagccgct ggatttcgat aacctacagg
    30901 cgacgctgga aaaggcgctc gcgcatacgc acagtattga tgctgaaaca cctgcggtga
    30961 ctgccagcca gttcggtatg gtcggtaaaa gcccggcgat gcaacacctg ctcagtgaaa
    31021 tcgccctcgt cgcgccatcg gaagccacgg tgctgatcca cggcgattcc ggcaccggta
    31081 aagagctggt cgccagggcg attcacgcca gtagcgcacg tagcgaaaaa ccgctggtaa
    31141 cgctcaactg tgcggcactc aacgaatcct tgctggaatc tgaattgttc ggtcacgaaa
    31201 aaggggcgtt tactggagcc gataaacgcc gggaagggcg ctttgttgag gcggacggcg
    31261 gcacgctgtt tctcgatgaa attggcgata tctcgccgat gatgcaggtg cgtctgctgc
    31321 gtgcgattca ggagcgcgaa gttcagcgtg tcggtagcaa ccagactatc tcggttgatg
    31381 tccggctgat tgcggcgacc catcgcgatc ttgccgcaga ggtgaatgcc gggcgttttc
    31441 gccaggatct ctactatcgc ctgaatgtgg tggcgattga agtaccatcg ctgcggcaac
    31501 ggcgggaaga tatccctctg ctggctggcc attttcttca gcgctttgcc gagcgtaatc
    31561 gcaaggcggt aaaaggcttt acgccacagg cgatggattt gctgatccac tacgactggc
    31621 cgggaaatat tcgtgaactg gaaaacgcgg tggaacgggc ggtggtgctg ctgaccgggg
    31681 aatatatttc cgaacgcgag ctgccgctgg cgattgccag tacgccgatc ccgctggggc
    31741 aaagtcagga tattcagccg ctggtggaag tggaaaaaga ggtgattctg gcggcgctgg
    31801 agaaaacggg cggcaacaaa accgaagccg cccgtcagtt agggatcacg cgcaaaacgc
    31861 tattggcaaa actgtcgcgt tagttctgct cgcgttcgat ggcgcgccaa ccgatatctt
    31921 tccggcagaa gcaatcatcc cagtggatat cggtcattaa ggcgtaggcg cgtttctgcg
    31981 cttctgccac ggtatgaccc agcgcggtga cgcacagtac gcgcccgccg ctggttacca
    32041 cctgctcgtc atccgccagt tttgtgcccg cgtggaacac tttgccgtca gccacttctt
    32101 ccagcggcag gccgtggatc acatcgccgg tgcggtaatc gcccggatat ccacccgcag
    32161 ccatcaccac gctgagagaa gcacgttcat cccactctga cgttttctcg tccagtttgc
    32221 cttcacaggc cgccaggcag agctcaacca gatcggactt catgcgcagc ataatcggct
    32281 gggtttccgg atcgccaaag cggcagttaa attcaataac cttcggattg ccctgtttgt
    32341 cgatcatcag gcccgcatag agaaaaccgg tgtaggtgtt gccttccgcc gccatgcctt
    32401 tcacggttgg ccagatgata cgttccatgg tgcgctgatg aacttcatcg gttactaccg
    32461 gcgcggggga gtaagcgccc atcccgccgg tgtttggtcc gttatcttta tcgcctacgc
    32521 gtttgtgatc ctggctggta gccatcggca gcacatgctc gccgtccacc atcacgataa
    32581 agctcgcttc ttcgccatcg aggaactctt cgataacgat gcgatgaccc gcgtcgccaa
    32641 aagcgttgcc cgccagcata tcgtgaacag ccgcttccgc ttcttccagc gtcatcgcca
    32701 cgataacgcc tttcccggca gccagaccgt ccgctttaat gacgattggc gcgcctttct
    32761 cacgcagata caccagcgca ggttctacct cggtgaagtt ctggtattcc gccgtaggga
    32821 tcttatggcg ggccaggaaa tctttggtaa acgcttttga gccttccagt tgggccgcac
    32881 ctgcggttgg gccgaagatt ttcagcccgg cggcgcggaa ggtatcgacc acgcctttca
    32941 ccagcggcgc ttccgggccg acgatggtca gatcaatctt ttcgttttgt gcgaaatcca
    33001 acagcgccgg gatatcggtc acgccgatag caacgttttg cagcgcgggt tccagcgcag
    33061 tgcctgcatt acccggagca acaaaaacag tctcaaccag cggcgactgg gccgctttcc
    33121 aggccagcgc gtgctcgcgc ccgccgttac caatcactaa tactttcatc tattgctcca
    33181 ttaatggcgg aagtggcgca tgtcggtgaa gagcatcgca ataccgtgct cgtcggcggc
    33241 ggcaatcact tcgtcatcac ggatggaacc gccaggctgg attacgcagg tcacgcccgc
    33301 agcggcggcg gcatcaatac cgtcgcggaa cgggaagaac gcgtcagaag ccatcgagga
    33361 acctttcact tccaggcctt catcggcagc tttaataccg gcgattttcg cggagtacac
    33421 gcggctcatc tggcccgcgc caataccgat ggtcatattg tttttggcat agacgatagc
    33481 attggatttc acgaatttcg ccactttcca gcagaacagc gcatcacgca gttcctgttc
    33541 ggtcggctga cgttgggtga cgacgcgcag ttcttccgca ccgaccatcc ccaggtcacg
    33601 atcctgaacc agcagaccgc cgttcacgcg tttgaaatca agacccggaa cacgctcgcc
    33661 ccactgaccg caggtcagaa cgcgtacgtt ctgcttggcg gcggtgattt tcagggcttc
    33721 ttcgctggcg gaaggcgcaa taatcacttc gacaaactga cgagaaatga tggcctgtgc
    33781 ggtttccgca tccagctcgc ggttaaaggc aatgatgccg ccgaatgcgg aggttgggtc
    33841 ggttttgtac gcgcgatcgt aagcatcaag aatggaattg ccgatagcta caccgcaagg
    33901 gttggcgtgc ttcacaatca cacatgccgg ctcggcgaac tctttcacgc actccagcgc
    33961 cgcatcggta tcggcgatgt tgttataaga gagggcttta ccctgaacct gggttgcggt
    34021 agcaacggag gcttctttca cattctcttc tatatagaag gcagcctgct ggtggctgtt
    34081 ctcgccgtaa cgcatatcct gcttcttaat gaagttcagg ttcagcgtgc gtgggaagcg
    34141 accggcggct tctttgcttt caccgtggta cgccggaacc atgctgccga agtagttggc
    34201 aatcatgctg tcgtaggcgg cagtgtgttc gaaggctttg atggcgaggt cgaaacgggt
    34261 tgcaagcgtc agcgatcctt cgttgtcatc catctcttta ataatggcgt cgtagtcgct
    34321 gctcttcacc acgattgcga catctttatg gttcttagcg gcggagcgca ccatggtcgg
    34381 gccgccgata tcgatgttct caaccgcatc ttccagcgag cagccttcac gagcaacagt
    34441 ctgggcgaac ggatacaggt taacaaccac catatcgata ggctggatct gatgttcttc
    34501 cataatggca tcgtcctggc cgcgacggcc cagaatgcca ccatgtactt tcggatgcag
    34561 ggtcttcacg cgtccatcca tcatctccgg gaaaccggtg taatcggaaa cttcggttac
    34621 cggcagacct ttttctgcta acagacgggc agtgccccct gtagacagca gctccacacc
    34681 gcgtgcggaa agtgcctggg cgaattcgac gataccggct ttgtcagaaa cactgagcag
    34741 agcgcggcgg actggacgac gttgttgcat ggtaaatccc ctgggtttga ctattacaga
    34801 gagcgttagc tgaatttttc gcgaaaaact cagctaacgc ccctaacggg gcatccttat
    34861 ttttcgcccg cattgtaacg aaaacgtttg cgcaacgctc gcgaattttt ctctttcaat
    34921 ggtgatcaca attttgacag tggttaccgt gggaaaaata cagaaattac attgatgatt
    34981 gtggataact ctgtgcgtaa aaaggtataa agcggggttt tgctggggaa tgcagcagtc
    35041 agtcattttt ctgcaatttt tctattgcgg cctgcggaga actccctata atgcgcct
//
LOCUS       DAETPZ010000038        34283 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.038, whole genome shotgun sequence.
ACCESSION   DAETPZ010000038 DAETPZ010000000
VERSION     DAETPZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34283
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.038"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(95..2191)
                     /locus_tag="K0W59_002046"
     CDS             complement(95..2191)
                     /locus_tag="K0W59_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3023079.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNLGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRLAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYARELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
     gene            complement(2193..2444)
                     /locus_tag="K0W59_002047"
     CDS             complement(2193..2444)
                     /locus_tag="K0W59_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3023080.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            2597..3160
                     /locus_tag="K0W59_002048"
     CDS             2597..3160
                     /locus_tag="K0W59_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023081.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            complement(3436..3858)
                     /locus_tag="K0W59_002049"
                     /pseudo
     CDS             complement(3436..3858)
                     /locus_tag="K0W59_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(3858..4451)
                     /locus_tag="K0W59_002050"
     CDS             complement(3858..4451)
                     /locus_tag="K0W59_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBI3023082.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTIIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            4598..5005
                     /locus_tag="K0W59_002051"
     CDS             4598..5005
                     /locus_tag="K0W59_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBI3023083.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            complement(5125..6117)
                     /gene="rpoS"
                     /locus_tag="K0W59_002052"
     CDS             complement(5125..6117)
                     /gene="rpoS"
                     /locus_tag="K0W59_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HBI3023084.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(6180..7319)
                     /gene="nlpD"
                     /locus_tag="K0W59_002053"
     CDS             complement(6180..7319)
                     /gene="nlpD"
                     /locus_tag="K0W59_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HBI3023085.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKIGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGAQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(7459..8085)
                     /gene="pcm"
                     /locus_tag="K0W59_002054"
     CDS             complement(7459..8085)
                     /gene="pcm"
                     /locus_tag="K0W59_002054"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HBI3023086.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(8079..8840)
                     /gene="surE"
                     /locus_tag="K0W59_002055"
     CDS             complement(8079..8840)
                     /gene="surE"
                     /locus_tag="K0W59_002055"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HBI3023087.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSSASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(8821..9870)
                     /gene="truD"
                     /locus_tag="K0W59_002056"
     CDS             complement(8821..9870)
                     /gene="truD"
                     /locus_tag="K0W59_002056"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HBI3023088.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARNALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(9867..10346)
                     /gene="ispF"
                     /locus_tag="K0W59_002057"
     CDS             complement(9867..10346)
                     /gene="ispF"
                     /locus_tag="K0W59_002057"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBI3023089.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(10346..11056)
                     /gene="ispD"
                     /locus_tag="K0W59_002058"
     CDS             complement(10346..11056)
                     /gene="ispD"
                     /locus_tag="K0W59_002058"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBI3023090.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(11075..11386)
                     /gene="ftsB"
                     /locus_tag="K0W59_002059"
     CDS             complement(11075..11386)
                     /gene="ftsB"
                     /locus_tag="K0W59_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HBI3023091.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(11580..11903)
                     /locus_tag="K0W59_002060"
     CDS             complement(11580..11903)
                     /locus_tag="K0W59_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HBI3023092.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(11953..12558)
                     /gene="cysC"
                     /locus_tag="K0W59_002061"
     CDS             complement(11953..12558)
                     /gene="cysC"
                     /locus_tag="K0W59_002061"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HBI3023093.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVAAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEEPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(12558..13985)
                     /gene="cysN"
                     /locus_tag="K0W59_002062"
     CDS             complement(12558..13985)
                     /gene="cysN"
                     /locus_tag="K0W59_002062"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HBI3023094.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(13987..14895)
                     /gene="cysD"
                     /locus_tag="K0W59_002063"
     CDS             complement(13987..14895)
                     /gene="cysD"
                     /locus_tag="K0W59_002063"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HBI3023095.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15147..16184
                     /gene="iap"
                     /locus_tag="K0W59_002064"
     CDS             15147..16184
                     /gene="iap"
                     /locus_tag="K0W59_002064"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HBI3023096.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(16330..17421)
                     /locus_tag="K0W59_002065"
                     /pseudo
     CDS             complement(16330..17421)
                     /locus_tag="K0W59_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(17615..17767)
                     /locus_tag="K0W59_002066"
     CDS             complement(17615..17767)
                     /locus_tag="K0W59_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBI3023097.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(18031..18765)
                     /gene="cysH"
                     /locus_tag="K0W59_002067"
     CDS             complement(18031..18765)
                     /gene="cysH"
                     /locus_tag="K0W59_002067"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBI3023098.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(18839..20551)
                     /gene="cysI"
                     /locus_tag="K0W59_002068"
     CDS             complement(18839..20551)
                     /gene="cysI"
                     /locus_tag="K0W59_002068"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HBI3023099.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(20551..22350)
                     /gene="cysJ"
                     /locus_tag="K0W59_002069"
     CDS             complement(20551..22350)
                     /gene="cysJ"
                     /locus_tag="K0W59_002069"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HBI3023100.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            22669..23031
                     /gene="queD"
                     /locus_tag="K0W59_002070"
     CDS             22669..23031
                     /gene="queD"
                     /locus_tag="K0W59_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HBI3023101.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            23109..24380
                     /locus_tag="K0W59_002071"
     CDS             23109..24380
                     /locus_tag="K0W59_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBI3023102.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPSWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            24371..24631
                     /locus_tag="K0W59_002072"
     CDS             24371..24631
                     /locus_tag="K0W59_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBI3023103.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GGLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            24648..25223
                     /locus_tag="K0W59_002073"
     CDS             24648..25223
                     /locus_tag="K0W59_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HBI3023104.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEVF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(25371..26231)
                     /locus_tag="K0W59_002074"
     CDS             complement(25371..26231)
                     /locus_tag="K0W59_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBI3023105.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGTEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIDVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(26228..27007)
                     /locus_tag="K0W59_002075"
     CDS             complement(26228..27007)
                     /locus_tag="K0W59_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBI3023106.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRCGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(26985..28394)
                     /locus_tag="K0W59_002076"
     CDS             complement(26985..28394)
                     /locus_tag="K0W59_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3023107.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(28416..29870)
                     /locus_tag="K0W59_002077"
                     /pseudo
     CDS             complement(28416..29870)
                     /locus_tag="K0W59_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
     gene            complement(29940..30726)
                     /locus_tag="K0W59_002078"
                     /pseudo
     CDS             complement(29940..30726)
                     /locus_tag="K0W59_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            31044..32321
                     /locus_tag="K0W59_002079"
     CDS             31044..32321
                     /locus_tag="K0W59_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3023108.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     HGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            32348..33826
                     /locus_tag="K0W59_002080"
     CDS             32348..33826
                     /locus_tag="K0W59_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBI3023109.1"
                     /translation="MSKKYIIGIDGGSQSTKVVIYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRHILFDVQMPGTVLGHITPQGALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         8283 a         9076 c         8423 g         8501 t
ORIGIN      
        1 catcatcaat atcagtcata gtatttcctc ggttatttca aatcagcttt gtttccttta
       61 tttaacagtc cgttcccgcg gactgttttt ttattcaggc cagcggatca tcttcttcct
      121 gcatggcaaa aagtttttgc atccccagcc ggaaatcgcg ttcaaattct tcgtcatgct
      181 cttcttcata ttccggttgc tgaactgcca ccacattacc aaccgggcga taaacattac
      241 tcagccaggg ttcagcctgt ttgtgttcca gaatctgaat accttcatct tcagcatcac
      301 gaattttttc ctcagcgcgt ttgcgcattc ctttaatacg ctgctgttgt ttgtgatatt
      361 cagcactgac agcaaaggct gcgcgtttat tgccgttcca tatcgcctca cagataaagc
      421 tgccgtcctt gcgacgtacc gtaattcgct cggcgtcatg aatgtcataa ctgataagca
      481 ctttgcgacc gtgttcgtca cgcaactccc gcgcgtagta aatattgttc agccactgaa
      541 tttcgcagcg tcttaccggt cgttccacca ttgggcggaa catgtcgcgc agctcaagat
      601 cactcagcca ctcaatttcg gtgtcttctt ctgccaggcg tttttttctg aactctgtcg
      661 ggctgtaatg ccgtccgttc ggtttcatcg gcagctcaga atgcgggcgg ttgttgtacc
      721 attccacgcc ttcacgaata gcctcaataa gctccgccca cgatggcagc tcgcgcattg
      781 ccgctttctg gcggtcgttc agaggcttgt cctgttgcag ggcgttaaac gctgaacgca
      841 gatcacggtt aagtacacgc aacgattccc ggtcagcacc cttgccaaaa taggtgcgat
      901 acctcctggc tatacgcatc ggcagtgtcc ggttcaggcg ttcaataatg ccgcgtccct
      961 gtggattacc ggcaataccg gtagggtgtt taatccccag gcgtggcaga atacccacga
     1021 cctctttgtc cagcatgtca gccgtttcac cggaaccgtt atccgagtaa tacaggaacg
     1081 gttttccgtg gttgcgaatg ccgtgctgta tcgcaccggc aacggcgaac acgttttcgg
     1141 caaggtcaag actccagcca acaacaaacc gtgttccacc atcgatcaca aacgtgactt
     1201 ccggtgagaa tggacgcccg tggatcggat gcgcacattt cagcttcata ccgtgaccgt
     1261 caccaatcca gacataattc accggcatag ctgtccagtc gcggcgggtg aatccttcaa
     1321 gctgacggta ttcactgccg gtaacgcgcc ctttttgttt caccacttcc ggcagttttt
     1381 tcatggcata ccggacaaca tcataggaag gcatcacctc cagcatgtac ggttcgtccg
     1441 catggcgacg ctgccattct gcgacaaaat cctcgtaggc ttctgacatg ggtctgccgt
     1501 tcacctggcg atactgcgcc agaaattcag gcagccagct gatttcttcc ggttttattt
     1561 cgtcgcgttt tcccggtgcc agtaacagca gccgttcggc ggcgttctgc gactggttgt
     1621 atttcagaac ccactggtaa agtggatcgc ggctgatagt acgattagcg ccacgttttg
     1681 cattggcgtt aactgcggcc tgttgcaggc gctccggcag ctcaccttta cgggcttttt
     1741 caacaagaaa gttaatggct ttcagtcgcc cgaggttacc ggcgctttcc agcttcagca
     1801 cttccaccac cagcgcagct cttgcttcag atacccagcg ctggttttcg gtcagcgcat
     1861 tcaggcgttc ttccatcagt tgtggagaac cgcgatatgc ttcgattgcc tggacagcgt
     1921 caggattgcg tgtttttctg gtaacagctt tcacttcaga aacattgttc tctgtagcca
     1981 ccagctgttc cacatagcgt tcacgcagtg cctttcgtgt tatttcaggc agacaatcaa
     2041 tgtggtactc aatggctttt gtgccttccc gacggcgact aacgtcacca agatcacgag
     2101 aaaaacgctg taacgcctgt cggatgcctt ttgtggtggc tggcattcct ggtacgccga
     2161 gcaggtcatt tacggtaacg tacgactgca tatcacgacg ccttcctgat aaatccatcg
     2221 ttacgatacc ggctgggcca gatgatttct ggcggtacac ctatagcgtc agaaattacg
     2281 cgttcgtatt ttttgtttgg tgtgcgaact accgctttca gtgaatcctg agcataacca
     2341 gcatggagtg aaagctcccg gaaggaagac cacccgttat ctttgagcgc cagctgaatc
     2401 ttcagcgaag gccaatcgcg gtcactatct tctctttgtc tcatctttcc tttaacctac
     2461 aaaataaccc gcgcggatat ccgtatggat aaagattaag ataccaaaga ttccctataa
     2521 agggttcttt ggtgctttta tggtttattt tttaatttca ttatttatca atgaattaga
     2581 tagaaaggaa ccaagaatga gaaaagcacc ccaaggttct tttcctgccg atggaaaaga
     2641 accgattatc gaaagaatct ttaggttagt agaacgatac ccttcaagaa atgcggctgc
     2701 tcgggcatgg gggatcaatg aaggcacttt aaaaaattat tacaacaggc gggattacgc
     2761 accgatacca cgcccgcatc agttaaaaaa gatagcagaa agcgaaaatg ttaccttgga
     2821 atggctcatg acgggagagg gtgatgttga actaaaagat tccaaagaac ccaaaaaaga
     2881 gaatgcggat aatgttgatg ctcaaattct aatgttcttg tcatttctta aaccacaaga
     2941 aaaacaacaa gtagctgatg ttcttgggag aaaaggagcg gagcaattac tcattcttct
     3001 ggatgagcac atccaagagt tgcacgctct agttggagtt cgtagagcca tagcgttaag
     3061 ccttggtaat ttgccagacg aaaaggttag agagatttac gcgttgtccg aagctaaggg
     3121 cgatcactta aatctacctc agaaagcagc cagcgcataa cccagtgcaa gtgcctagct
     3181 gtgctaaaat tacattggtt ttggcgcttg cacccatcag cgctataata aacacctgaa
     3241 tcgtttttga acggtcttta aaggctattg taagaacctg tgagttatct tgaatgtttt
     3301 attgatatcg aaccgtttaa catttttgga ctcgctttat cttgaaaacc ttgagttcct
     3361 aacttgttga ttccaatcta ttcccgtctt ttttaatctc ttcccggtta tcttgaataa
     3421 attacctagt catacgagat agaaaccgcc atcgccatca ttcaggcgaa acagcggcag
     3481 aatatcgaac aggttgatct cttcgccatc gacggtgttc tgcgcccagg ctggattggc
     3541 gcggcgctcc ggggcaatcg ggaagttatc ccagcggcgg ataaactcat caatctgctt
     3601 tttgaccggg gtgtttggtg gcaggccaag ggaaatcgcg tggttctgcc aggaaccgat
     3661 ggtattcatc gccacgcggg catcggtaaa gccgcgaata ttatcaaacc acagcgcggg
     3721 cgcgccgtcg ccgatacgcc cggtggcgtt agctgctgct gccagatccg gctcagcgtt
     3781 cacttcttcg ctgattttca gtaactggcc gtggtcatca agcgcctgta aaaagctgcg
     3841 taaatcatca aatgccatta ttcattctcc tgagaaaaat tccgggcctg cggcaatcct
     3901 tgccagcgcc tggcgtgggg atgttcgagg ccaaattgat ccagcacgcg ggctaccaca
     3961 tggtggacaa tgtcatctac cgtttcggga tggttataaa aggcaggcat cggcggcacc
     4021 atcgccacgc ccatgcgtga aagtgcgagc atattttcga gatggatggt gctaagcggc
     4081 atttcacgcg gcaccagcac cagtttgcgg ccttctttga gcacgacgtc cgccgcgcgc
     4141 cctaccaggc catcggcgta accagcgcgg ataccggcga gcgttttcat actgcacgga
     4201 ataacgatca tgccgtcagt acgaaaagaa ccggatgaga tgatcgccgc ctgatccgcc
     4261 gggttatggc tgaagtctgc gagggcagca acatcgcgag cgctgtaagg cgtttccagt
     4321 tcaatgatgg ttttcgccca cttcgacatc accagatgag tctcgacatt cggcatctca
     4381 cgcagcgctt gcagtaatgc cacaccaaga ggcgcaccgg tagcccctgt catcccgacg
     4441 atcagtttca ttgcttattc tcttaattat tcgcatacga acgttattat taacatactc
     4501 cttcatttcc acagcggcaa ggattgaaaa gtaacaacat cttcttcgtc actaaaaaac
     4561 cgctatgata gcgacagaga attagcccgg agtttacatg gcgttacgaa ataaagcgtt
     4621 tcatcagtta cgacagcttt ttcagcagca cacggctcgc tggcagcacg agttacctga
     4681 cctgaccaaa ccacagtacg cggtgatgcg cgccattgct gataaacctg gtattgaaca
     4741 ggtggcactg atagaagcag cagtcagcac caaagcaacg ctagcagaaa tgttggcaag
     4801 aatggaaaat cgtggcttag tcagacgtga acatgacgtc gctgacaagc ggcgacgctt
     4861 tgtctggctg actgccgaag gagaaaagct acttgctgcg gcgataccta ttggtgacag
     4921 cgtggatgag gaatttttgg ggcgtttgag tggagaagag caggagctgt ttatgcagtt
     4981 ggtgcgcaag atgatgagca catagaatgc tgattgccgg gtaggacgct gacgtgtctt
     5041 atccaggcga caaaaacaca agcttcccaa acaaaagaaa aaggccagcc tcgcttgaga
     5101 ctggcctttc tgacagatgc ttacttactc gcggaacagc gcttcgatat tcagcccctg
     5161 cgtttgcagg atttcgcgca aacggcgcag gccttcaacc tgaatctggc gaacacgttc
     5221 acgggtgagg ccaatttcac gacctacatc ttccagtgtt gccgcttcgt accccagcaa
     5281 accgaatcga cgtgccagta cttcacgctg tttggcgttc agctcgaaca gccatttgac
     5341 gatgctctgc ttcatatcgt catcttgcgt ggtatcttcc ggaccgttct ctttttcatc
     5401 ggccaggatg tccagcaacg ctttttcgga atcaccaccc agcggggtgt ctaccgaggt
     5461 aatgcgctcg ttaagacgaa gcatacggct gacgtcatca actggcttat ccagttgctc
     5521 tgcgatctct tccgcacttg gttcatggtc cagcttatgg gacaactcac gtgcggttcg
     5581 caggtaaacg ttcagctcct ttacgatgtg aatcggcaaa cgaatagtac gggtttggtt
     5641 cataatcgcc cgttcaatcg tctggcgaat ccaccaggtt gcgtatgttg agaagcggaa
     5701 accacgttcc gggtcaaact tctctaccgc gcggatcagc cccaagttgc cctcttcgat
     5761 aaggtccagc aacgccagac cacgattgcc ataacggcgg gcaattttta ccaccagacg
     5821 caagttactc tcgatcatcc ggcggcgaga ggcgacatct ccacgcagtg cgcgacgcgc
     5881 aaaataaact tcttcttcgg ccgttaacag tggtgaataa ccaatctcac caaggtaaag
     5941 ctgagtcgcg tccaacacac gctgtgtggc tccctgcgat aacagttcct cttcggccaa
     6001 atcgttatca ctgggttcct cttctactaa ggccttttcg tcaaaaacct caactccgtt
     6061 ctcatcaaat tccgcatctt catttaaatc atgaactttc agcgtattct gactcataag
     6121 gtggctccta cccgtgatcc cttgacggaa cattcaagca aaagcctggt tccgccgatt
     6181 tatcgctgcg gcaaataacg cagcgggttt acggatttcc ccttgtaacg aatttcaaaa
     6241 tgcaagcgtg ttgaactggt tccggtgcta cccatggttg ctattttttg ccccgccttc
     6301 acttcttgtt gttcccggac cagcattgtg tcgttatggg cgtaggcact caggtaatca
     6361 tcattatgtt tgatgataat cagattaccg tagccgcgca gcgcgttacc agcataaaca
     6421 acgcggccat ctgcggtcgc gataattgcc tgtcctttgc tgcctgcgat atcaatcccc
     6481 ttgttgcccc cctcagaagc gccaaaggtt tcgatcactt tgccctcagt cggccagcgc
     6541 caggtggaga taggcgtact ggttgatgta ctgctgacag tcggctcggt tgtgcttgct
     6601 gttggtaccg ttacaggcgc tgtgaccgtg gtcgcagttg gcttgttgtt cggcaacatt
     6661 ttgttagcac tctgtgcacc cgaagactca gaatacgtaa ttgtcggttg cgacgcaaca
     6721 gcaacggtgg aattttgtgc aggcttgatc acaactcctt gctctgctgc gtcggcctgg
     6781 gtaatagcat ttccgccagt gattggcgta ccggaagcat tacccacctg caaggtctga
     6841 ccaacgttca gcgcgtatgg tgcctgaata ttgttgcgct gagcaaggtc acggaaatcg
     6901 ttgccagtaa tccaggcgat atagaaaagt gtgtcacctt ttttcacggt ataggtactg
     6961 ccgctataac tgcctttcgg aatgttccca tactgacggt tatagacgat gcgtccgttt
     7021 tccatctgta ccggctgctg agctactggc tgcactggct ggatttgcgg ttgttgagta
     7081 gcctgaattt gtggctgctg taccggctga atttgcggtt gctgcgctgt agacgtcgtc
     7141 cctattttcg gcggcggcgt aatcaacata ccagaattag tatttgcagg cgcattgcca
     7201 ttaacggagc tgaccggtgc cggtggattt gaagtgtcag aacagcctgc cagccatagc
     7261 gaaaccagtg acaaagccgc aatgcggcga acggtgaatt ttgggcttcc cgcgctcatt
     7321 tatcccccag gaaaaattgg ttaataacca gtgacataat taccgtgcaa ggcaccctac
     7381 tgaacactgg aaaagatgtt cacgatacgc tgacctacgg caaaataacc aggaaaaatc
     7441 caggtatttc ctcacgtttt aagccagctc acccttcact aaagggacaa agcgcacggc
     7501 ctccacggta tcgataataa attcgcctcc ccgacgacgc acccgtttca aatactggtg
     7561 ctcctcccct acgggtaaga cgagaatccc gccttcgtcc agctgcgtca ttagcgcagt
     7621 tggaatttcc ggcggtgccg ccgtaacaat gatagcgtca aacggcgcac gtgcctgcca
     7681 accttgccat ccatcaccat gacgggttga aacattatgt aaatcaagat ttttcaggcg
     7741 gcgacgtgcc tgccactgca agcctttaat ccgttcaacc gagcaaacat gctggacaag
     7801 atgcgccagg attgccgttt gatatcccga accagtgcca atttccagca cccgcgactg
     7861 cggcgtcagc tcgagtaatt cggtcattcg cgccaccata tacggctgcg aaattgtctg
     7921 cccctgacct atcggcaagg cgatattgtc ccaggctttt tgttcaaacg cttcatcaac
     7981 gaatttttca cgcggcacgg cggcaagtgc attcagcacc tgctcatcct gaataccttg
     8041 cgcacgtaat tgatccagaa gtgcttgtac gcgtctgctt accattgcgt gccaactccc
     8101 acgctgttta accagtctga aaccacatct tgcgcgctat gcgcagttaa atccacatgc
     8161 agcggcgtga tggagacata gccctcatct accgcagcaa aatcggtccc cggaccggca
     8221 tcacatttac cgcccggcgg gccaatccag tacagcgtat tgccgcgcgg atcttgctgc
     8281 gggatcacct gatctgccgg atgtcgtgta ccgcagcgcg tcacgcgaat acctttgatt
     8341 tgatccaagg gtaaatccgg aacgttaata ttaagaatac gtccggtgcg cagcggctct
     8401 ttacacagtg cgcgcaaaat tgaacaggtt accgccgcgg cagtgtcgta atgtttatgc
     8461 ccgtcaagcg agacggccag cgccggaaaa cctaaatgac ggccttccat cgcggcggct
     8521 accgtaccgg aataaataac atcatccccc agattcggcc cggcgttaat tccggacaca
     8581 acaatgtccg ggcgcggacg catcagagca ttcacgccaa gatagacaca atcggtcgga
     8641 gttcccattt gcacagcaat atcaccattt tcaaaggtaa acgtgcgcag ggaggattcc
     8701 agtgtcagag aatttgaagc gctgctgcgg ttacgatcgg gggcgaccac ctgaacgtca
     8761 gcaaactcac gcaaggcttt cgccagcgtt tgtataccgg gtgcatgtac cccgtcatca
     8821 ttactcagca atatgcgcat aatcacctgt tgtgttgata agttccctta caacgctggt
     8881 tgcaaaacta cccgccggaa gccagaaacg gatctctacg gtgacgtcat cccaccagtt
     8941 ccagcttaat tgttgcggat acagcagcat cgctctgcgc gcggcttcaa ctttttcgcg
     9001 caccagtaaa gcttgtaatt cagtttctgc ggcgacagct gcttgttcga atgccagcgc
     9061 ttcacgctga gttccccatt cgccactgcc cggcaatgcg gcggttatca tcagctcttt
     9121 atcgttgacg cgacgctgta attccgccag ttcttcggtg gttgctacaa accagctacc
     9181 acgtccggct aattgtagcg catcgccgtc aacaacttga ttaacgtctg cttttttgag
     9241 gcgctcagca acaatctgat taaacaacgc attgcgggct gccgacaacc aaaaactccg
     9301 tttattgcga tcgcgcaccg gagtattggt ttgcgcccag cgcagcgcgc cctgcaagtt
     9361 gctaccgcca atcccaaaac gttgggcacc gaagtagttc ggtacacctt ttacgcaaat
     9421 atcgatcaga cgttgttcaa cgtcatcgcg attgctcact tcgcgcagaa ccagggtaaa
     9481 ggcgttacct ttcagcgccc ctaaacgcag cttgcgcttg tgccgcgcat actccagcac
     9541 ctggcagcct tccagttgaa aggcgctcag atcgggcatt tccttgcccg gcacgcgagc
     9601 acataaccac tgttccgtta cagcatgttt gtctttttgc ccagcgaagc tgacttcacg
     9661 ggaatgaatt ttcaggaatt tcgccagtgc atccgccaca aaacgggtat tgcagccgtt
     9721 tttgaggatt ctaaccagaa tatgctcacc ttcaccatca ggctcaaagc ccaaatcttc
     9781 caccaccaca aagtcttccg gattggcttt cagcagcccg gtgccttgcg gtttaccgtg
     9841 gaggtaagtg agattatcaa actcaatcat tttgttgcct taatgagtag cgccaccgct
     9901 tcacaggcaa tcccttcccc acgtccggta aatccaagtt tttccgtagt agtggctttc
     9961 acgttaacat catccatatg gcagccgaga tcttcggcaa taaacacgcg catttgtgga
    10021 atgtgcggca acatcttcgg tgcctgagcg atgatagtga catcgacgtt gccaagggta
    10081 taacccttcg cctgaatacg acgccaggct tcgcgtagca gctcgcggct gtcggcacct
    10141 ttaaatgccg gatcggtatc cgggaacagt ttgccgatat cccccagcgc cgccgcgcca
    10201 agcaatgcat cggtcaacgc atgaagcgcc acgtcgccat cagaatgcgc cagcaatcct
    10261 ttttcgtaag gaatgcgtac gccaccaatg ataattgggc cttcaccgcc aaaggcgtgt
    10321 acgtcaaaac cgtgtccaat tcgcattatg tattctcctg atggatggtt cgggtgaggt
    10381 aaaactcggc cagtgccaaa tcttccgggc gcgtgacttt aatgttatcc gcgcggcctt
    10441 cgaccaactg cggatgaaat ccgcaatatt ccagcgccga ggcttcgtcg gtaatggtag
    10501 cgccttcatt tagagcgcgc gtcagacagt catgtaacag ctcacgaggg aaaaattgcg
    10561 gcgtcagcgc gtgccataag ccgttgcgat caacggtatg agcaatggca tttttgcccg
    10621 gttcggcacg tttcatagta tcgcgcactg gtgcggcgag gatccctccc gtgcggctgg
    10681 tttcgctcaa cgccaacaat cgcgcgaggt catcctgatg caaacaagga cgagcggcgt
    10741 catgcaccaa tacccactgc acatcgccag cggctttcag acctgccagc acggaatcgg
    10801 cacgctcatc accgccatct acaacggtga tttgcggatg attcgccaga ggaagttgtg
    10861 caaaacggct atcgccagga cttatggcaa tgacgacacg tgtcacccgg ggatgcgcca
    10921 gcagcgcatg caccgagtgt tcaagaatgg tttgattacc gattgagaga tattgcttag
    10981 gacattccgt ttgcattcga cggccaaatc cggccgccgg aaccacggcg caaacatcca
    11041 aatgagtggt tgccatgtta attcccgggc tgatttatcg attgttttgc cccgcagact
    11101 gtgcgcgctt cgacgcgtca ggcaccagac gataaaaagt ttcgcccggc ctggtcatgc
    11161 tgagttcatt acgcgcacgc tcttcgagcg cctcctggcc gccattgaga tcgtcaattt
    11221 cggcaaaaag ttgatcgttt cgcgctttaa gtttcgcgtt tgtagcttgc agtgccgcca
    11281 catcatcatt gacgcgggta tagtcatgta taccgttctt accgaaccac agcgaatact
    11341 gtagccagac cagaatagcc agcaacagca gcgttagttt acccatcctg ccccctgaaa
    11401 aactgcatca tcatcccatg catccgaaga cgactctaca tcctctgttg gggataccgc
    11461 gacaacgcgg gcaaatgtac cacatttgtc cattgttacg tatacccagg gcgtgcagaa
    11521 cataatctca ttattagtta cggtttgaat tatgaacaga ggagacaaga aagtacaaat
    11581 tagcccagta gccacataaa cagtgcgcca aacataatgc ctactgtcac cagggtgaaa
    11641 acaatactgt agcgtagctt tccgtccatc aatgaatgca gcgcaatccc caccactacc
    11701 gcgacaggca tcagtgccag aaagaaaggc caggtgtaga taaagaagaa cagcgtgtta
    11761 gagccataaa tcaacatcgg catcgccagc gcaaataacc aggagataaa accgaccacg
    11821 gcaccaggca gtgaccatgt agtttcttca tcctcagtaa ggctgtcgtt atttgttagt
    11881 gtaatgttat ggctattacg catatttgat cctgttactt tgacgaaccg ggcatggaaa
    11941 cccggtggtg tctcaggatc tgataatatc gttctgtctc aacagatcta ataattgctg
    12001 taccaaattt gttactaatt gttcaccatt gagatgaatt tctgccgatt caggctcttc
    12061 gtaaacggaa tctattcccg taaagttgcg cagttcaccg gcacgcgctt tcttatataa
    12121 gccttttgga tcgcgggctt cgcaaatcgc cagcggcgta tcgacaaaca cttcaataaa
    12181 gcgcccttct cctacgcgtt cgcgaaccat ctggcgttcg gcgcggtgtg gcgagataaa
    12241 tgcggtcagc accaccagtc cggctgcaac catcaaattc gccacttcac cgacgcgacg
    12301 gatattctct ttacgatcgg catcgctaaa accgagatcg ctgcataatc cgtggcgaac
    12361 attgtcgcca tccagcagat acgtactgac gccgagttta tgtaacgcct cctccagcgc
    12421 cccggcgacc gttgatttac cggacccgga gaggccggta aaccacagca ctacaccacg
    12481 atgaccgtgg tgtagttcgc gttgttgcac agtgaccgga tgactatgcc agacgacgtt
    12541 ttcgtcatgc agcgccatta tttatccccc agcaaatcgc gcgcgcccca gtgcggaaag
    12601 tggcgacgaa ccagggcatt caattccagt tcgaatgcac tgaattcaga tgacgcagct
    12661 gttgcctggc taactggctc gtgcaccata ccggcaccca cggtcacatt gctcaggcga
    12721 tcgataaaaa tcagcccacc ggtaaccgga ttttgctgat agcgatctaa caccagtggc
    12781 tcgtcaaaag tgagatccac caggccgatg ccgttcagcg gcaggttttc aacttcgcgt
    12841 tgggtaaggt tattaatatc aacctgataa cgaatgccat caacacgagc acgcgtcttc
    12901 ttaccggcaa ttttgatgtc ataactctgg cccggggaaa gcggctgttc cgccatccat
    12961 accacatcca ccgacgcact ctgcaccgcc ggtaacgctt cgtctgccgc cagcagcaga
    13021 tcaccacggc tgatatcgat ctcatccgtc agcaccaggg tgatcgcttc tccggcaaag
    13081 acttcttcgc gatcaccatc aaaagtcacg atccgcgcga cgtttgattc cacaccagag
    13141 ggcagcactt ttacacgttg cccgacttcc acgcgaccgg atgccagcat tccggcgtag
    13201 ccacgaaaat cgagatttgg gcggttaacg tactgcaccg ggaagcgcat tggctgggca
    13261 tccaccactc gctggatctc cacggtttcc agcacttcga gcagtgtcag accgctgtac
    13321 cacggcatac tttcactttg cgaagccacg ttgtcacctt ccagtgcgga gagcggcaca
    13381 aagcggatat ccagattacc cggcagctgc ccggcaaagg tcagataatc ttcacgaata
    13441 cgggtgaacg tctcttcact gtaatccacc agatccattt tgttgatcgc cacgaccaga
    13501 tgtttgatcc ccaacagtgt ggagataaaa ctgtgacgac gggtttgatc gagcacgcct
    13561 ttacgggcat caatcagtaa gatcgccagt tcacatgtcg atgcgccagt cgccatattg
    13621 cgagtgtact gctcgtgccc tggggtgtcg gcgataataa atttacgctt ctcggtagag
    13681 aaatagcggt aggccacatc aatagtgatg ccctgttcgc gctcagcttg caggccgtcc
    13741 accagcagag ccagatccag cttttcgccc tgggtgccgt gacgcttact gtcgttatgc
    13801 agcgatgaga gctgatcttc gtagatttgg cgggtatcgt gcagcagacg accaatcagg
    13861 gtacttttgc cgtcatcgac gctaccacag gtcagaaaac gcagcaggct tttatgttgt
    13921 tgcgcaatca tccaggcttc gacgccgcct tcattggcga tttgttgtgc aagtacggtg
    13981 ttcatcttaa aaatacccct gacgtttttt cagctccatg gatcccgcct ggtcgcggtc
    14041 aatcacgcga ccctgacgtt cactggtggt ggaaaccagc atctcttcga tgatttccgg
    14101 cagtgtttgt gcatttgact ccaccgcacc ggtcagcggc cagcagccca gcgtacggaa
    14161 acgcaccatc cgttttttga tcacttcgcc cggttgcagg tcgatacggt tgtcatcaat
    14221 catcatcaac ataccgtcgc gttccagaac cggacgttcc gcagcgagat acagcggcac
    14281 aatgtcgata ttttccagcc agatgtattg ccagatatcc tgctcggtcc agttagagag
    14341 cgggaagacg cggatgcttt cgcctttgtt aatttgcccg ttgtagttgt gccacagctc
    14401 cgggcgctga tttttcggat cccagcgatg gaagcggtca cggaaagagt agattcgctc
    14461 tttagcgcgg gatttctctt cgtcacggcg cgcaccaccg aaggcggcat caaaaccgta
    14521 tttgttcagc gcctgtttca ggccttcagt tttcataata tcggtatgtt tcgcgctgcc
    14581 gtgcacgaat ggattaatcc ccatcgccac gccttccggg tttttatgca ccagcagctc
    14641 gcagccgtag gctttcgccg tacgatcgcg gaactcatac atctcgcgga atttccagcc
    14701 ggtatcgaca tgcagcaacg ggaaaggcag cgtacctgga taaaacgcct tgcgcgccag
    14761 atgcagcatg acgctggaat ctttaccgat agagtagagc atcaccggat ttgagaattc
    14821 tgccgccacc tcgcgaataa tgtggatgct ttccgcctcc agttgccgca ggtgagtaag
    14881 tcgtttttga tccataaccg ttcctttgca ataccgctat tttcttgcca tcagatgttt
    14941 cgactatagg gagcgtaaga gaacgaatga aattaccaat tagaatgagt agttccttaa
    15001 cggaataacg atttggcaaa gctaatatca aaaagtgctt aaggcaccgg atttcgggcg
    15061 tttaggaaga tttgaaattg ttttagcgca gcggcagttt catactatgg cggtaaaaaa
    15121 atttgcatgg tatttaagga ctcactatgt tttccgcatt gcgccaccgt accgctgccc
    15181 tggcgctcgg cgtatgcttt attctccccg tacacgcctc gtcacctaaa cctggcgatt
    15241 ttgctaatac ccaggcacga catattgcta ctttctttcc gggacgcatg accggaactc
    15301 cagcagaaat gttatctgcc gattatattc gccaacagtt tcagcaaatg ggttatcgca
    15361 gtgatattcg gacatttaat agtcggtata tttataccgc ccgcgataat cgcaagaact
    15421 ggcataacgt gacaggaagt acggtgattg ccgctcatga aggcaaagcg ccgcagcaga
    15481 tcatcattat ggcgcatctg gatacttacg ccccgctgag cgatgctgac gcagatgcca
    15541 atctcggcgg actgacgtta caaggaatag atgataacgc cgcaggttta ggtgtcatgc
    15601 tggaactggc agaacgcctg aaaaatacgc ctaccgagta tggtattcga tttgtggcga
    15661 ccagcggcga agaggaagga aaattaggcg ctgagaattt actcaagcgg atgagtgaca
    15721 ccgaaaagaa aaatacgctg ctggtgatta atctcgataa cttaattgtt ggcgataaat
    15781 tgtatttcaa cagcggtgta aaaacccctg aagcagtaag gaaattaacg cgcgacaggg
    15841 cgctggcaat tgcacgcagt cacggaatag ccgcaacgac caatccgggt ttgaataaaa
    15901 attatccgaa aggcactggg tgttgtaatg acgcagaaat attcgacaaa gcgggcattg
    15961 ctgtactttc ggtggaagcg actaactgga atcttggaaa taaggatggt tatcagcaac
    16021 gcgcaaaaac acctgccttc ccggcgggaa atagctggca tgacgtaaga ctggataatc
    16081 aacaacatat tgataaggca cttccaggaa gaatagaacg tcgctgccgt gacgttatgc
    16141 ggataatgct accgttggtg aaggagctgg cgaaggcgtc ttgatgggtt tgaaaatggg
    16201 agctgggagt tctaccgcag gggcggggaa ttctaagtga tacccatcat cgcatccagt
    16261 gcgtccggtt tacttccggg aaaaccgcac cgtattgcgc cggtttatct ccgctggagc
    16321 ggggaacacc tctggcgaat agaaagcaag ccaggttttg accagtcgat cgtacatggc
    16381 attggccgtt gccattgttg gcaggccaaa aaacagcccc tgagcgttac ccgcagccat
    16441 caacctgtgc gccaggataa gcgccgcttc tgttttacct gcgccggtca catcttccag
    16501 aataaacaac tgtggtcctg gctgattgat atccagatcc agcacctttt gctgtaacgg
    16561 tgtcgtattc tcaataaaag gaaacagcgt attaattccg gtgaaaggcg cggtttctgc
    16621 ttgtggcgga aagacggtta atgcgttttg agcctgaact aaagcctttt gccagtaatc
    16681 ttcaatatcc attgcctgtg cgacgcgtgg aaaaaatcgc gttgacgacc ctgtccagtc
    16741 tgcaagtacg acggttgcag agatatacca ggaaagttgt tttataagtt caacgccctc
    16801 gtcatcatcc cagaatgtgg ggatctctat tcgcggaaac agtggcttga tttcaaggag
    16861 aaaatctcgt gctgcagctt tgtcttcagg cagaaaatta tccaattcat ccatacgatc
    16921 agggggccga ccatgatgcc ctgtcgtcac gggcatccac atttctatca ctcgcctaag
    16981 tttgcgcgga gagagcgaag atgaaggaag taactcctga cattcactta aataatgatt
    17041 ccacaaccag tagcccagcg ttgaatgaga gattttttcg taattttttc tggcaccttc
    17101 ctgaatcttg aattcagggg gcaggtaaag ttgttgaaaa gagcgggcaa attttccaat
    17161 atcgtgccag cacagcaacc aggcgaaaaa ttgagctgcc tgttccttat cagaaatccc
    17221 taattgacga aaataatcag ccagcccgaa gcaatttctt ttaaccatta aatagcccat
    17281 tgcggccaca tccagcaaat gccagcaaag aaggtgatag tcgtcgctac cctctttctc
    17341 gccacgtcgg ttttttcccc agaaatcaaa gaaagtcaca atatttttat ccttcagtaa
    17401 acttaaagga tatttacgca tattttaaaa actatctgtg atatatatca gccaataaaa
    17461 catttacatt atgaataaat taatgatttt catttgaaat tcataataat aaatagagac
    17521 tatatattca cataaaaaat ataaatttta aaaaagggtg caggagacca aaccatcaca
    17581 attcagtacg cggggtttta cccccgcgtg gtggctactt cttcgattcg taagcgagaa
    17641 ctgccttaaa ctccatacca cgttctctga tactcaactc acataacgag tcacctacca
    17701 tcaacgtgaa tcccagtact gtacaacaca gtacgatgat tgccacaagg gcatattttg
    17761 tcagcatgtc ttgcctccct gcaaagaaga gactaacatc cgaattgcta cgttcgaacg
    17821 cgagcctcag gttgatagaa atatcgcctg gggcttttgt ccatctggaa cctcgcgaat
    17881 gcttaacgcc agacagcctc aagcacccaa cgccattcta tacctgttaa ttttccctgc
    17941 gatgttcacc tttaaatgcc ggaatgctgc tcgcaaaata aaaacgcccc catcatttct
    18001 gacagaggcg tttaatttat ccggcaatat ttacccttcg tgtaatccac attccctttt
    18061 taagccaaag aaacgcgttt cttcttccgc catgccgggt tcccatttac gggttgtatg
    18121 ggtatcgccc accgataaat atccttcgtc ccataatggg tgatatttca ggccatgttt
    18181 ttgcaggtac tgataaatag ttcggttatc ccagtcgata atcggcagca ctttaaatac
    18241 gccacgctga attgccagca ccggtaaatt ggcacggctg ccggattgtt cacgccgcag
    18301 gccagcaaac cacgtttgcg cattcagttc tttcagcgcc cggttcatcg gttcgacttt
    18361 gttgatgtca ttgtactttt caatgccttc aacgccctgt tcccacagtt tgccgtagcg
    18421 tgcttcctgc caggcggcgc tttcggtagc gcggtacact ttcaggttga gcttgagttt
    18481 gtccgttaac tcgtcaataa agcggtaggt ttccgggaac agatagccgg tgtcggtgag
    18541 gatcaccgga atatccgggt gaatctgatt caccagatgc aggctcaccg ccgcctgaat
    18601 gccaaagctg gaagaaagta catattcacc gggcagatta tccagcgccc aggcaacgcg
    18661 gccttcagcg tccagttttt ccagttcggc gttagtttct gccagcgcca gaatgcgatc
    18721 taccttcggc agttcgttca gggcgtttag atcgagtttg gacatagatt cctcactgtt
    18781 tgccttgcct gatgcgacgc tcacgcgtct tatcaggcct acaagaccgg gctgatggtt
    18841 aatcccacaa atcgcgcgcc ggatcgagca ccgggcgaat gatgcccgca cgcaccgtaa
    18901 agtcgccgaa gccttcaccc gcttcgcgct ctttcgccca gcgccctatc agttcatcaa
    18961 gcgacgccag gatttccggc tcggtgatgt tttctttata catccgtggg atacgtgtcc
    19021 caatgcggtt gccgccaaga tgcaggttgt agcgacccgg tgctttaccc accaggccca
    19081 cttccgccag catcgcgcga ccacaaccgt tcgggcagcc tgttacacgc atcacgatat
    19141 gctcatcgct gacaccatgt ttcgccatta aattatcgat gttgtcgata aaagacggca
    19201 ggaaacgctc cgcttccgcc atcgccagcg ggcaagtcgg gaatgacacg caggccatcg
    19261 agttttcacg ctgcggcgtg acggcattca ttaacccgct ctctttggca attttctcga
    19321 tcttcgcttt ctcgctttcc ggcacaccgg cgatgatcag attctggttc gccgtaatgc
    19381 ggaaatcacc tttgtggatc ttcgcgatct ccagcaggcc agttttcagc ggacgccccg
    19441 gataatcaag gatgcgacca ttttcgataa acagcgtcag gtgccagtta tcatcaatgc
    19501 ccttaaccca gccaatacga tcgccgcgtc ctgtaaattc atacgggcgg atcggttcaa
    19561 atttgatccc cgcgcgacgc tccacttccg ctttaaacgt ctcaaccccc acgcgctcca
    19621 gtgtgtattt tgttttggca tttttacgat cggttcggtt accccagtca cgctgagttg
    19681 tcacgacggc ttccgccacc gctagcgtat gctctagcgg cagatagcca aactcactcg
    19741 ccgtgcgggc gtaggttttc ttgttaccgt gttcgataga aagcccaccg cccaccagca
    19801 ggttgaagcc caccagcttg ccgttttccg cgatcgccac gaagttcatg tcgttggcgt
    19861 gcagatcaat atcgttctgc ggcgggatca ctaccgtggt tttgaactta cgcggcaggt
    19921 aggtctgacc aaggatcggt tcttcatcgg tggtggcgac tttttcctga tcgagccaga
    19981 tctccgcata cgcgcgggta cgcggcagca gatgttcaga aatcttcttc gcccactcgt
    20041 acgcttctgc gtgcagctgc gactcgtaag ggttcgaggt gcagagtacg ttacggttca
    20101 tgtcgttagc agtcgccagc gcatcaagac cgaccgagtg cagcatctgg tgcaccggtt
    20161 tgacgttctt tttcagaatg ccgtggaact gaaacgtctg gcggttggtc aggcgaatgc
    20221 tgccatagat ggtgttttca ccggcaaatt tatcgatcgc ctgccactgt ttggtggtga
    20281 taactccacc cggcagacgg cagcgcaaca gcatcgcgtg gcgcggctcc agcttctgtt
    20341 cagcacgttc ggcgcggatg tcgcggtcat cctgctgata catgccgtgg aagcggatca
    20401 gcaggaagtt atcgccctta aagccgccgg tcagaccgtc gtttaaatct tccgcaatgg
    20461 tgccgcgcag gtagttgctt tcaagcttca tgcgctcggc gtctgtcagt tttccttcga
    20521 ccactaaagg ccctggatgt ttttcgctca ttagtagaca tctcgctgat aacggcgctc
    20581 tacgcgcagc tcacttaaaa attcatccgc cgcttcggtg tccatgccac caaattcggc
    20641 aatcacttcc agaagtgcct gctcaacgtc tttcgccatg cgattagcgt cgccgcagac
    20701 ataaatgtgg gcaccgtcat tgatccagcg ccacagctcc gcgccctgtt cgcgcagttt
    20761 atcttgtacg taaacttttt ctttttggtc gcgcgaccag gcgagatcga tccgcgtcag
    20821 cacaccctct ttgacgtagc gctgccactc cacctgatac aggaagtctt ccgtaaaatg
    20881 cggattgcca aagaacagcc agtttttacc tggcgcttcg tcagcggcgc gttgctgcat
    20941 aaaggcgcgg aacggcgcaa taccggtgcc tgggccaatc ataatcaccg gggtttccag
    21001 gttagcgggc aggcggaagt tatcgttatg ttcgataaat acgcggactt cgccctcttc
    21061 ttccacgcgg tcagcgagga agctggaggc accaccggca cgggcgcggc cttccacgtc
    21121 gtaacgcacc acaccaacgg tgacgtgtac ttcgttctcg acttccgcct gcgaggaggc
    21181 aatggaatag agacgcgggg tcagcgggcg cagcagatta attagcgctt cggcatcaag
    21241 ctgtgccggg gagaaacgca ccatgtcaac aatcagcgtc gtcgcggcgt aatgctgtaa
    21301 cttcgcttta tcgcccacca gcggcagcag tgtttcactg cgggtaagcg tggcgtaatt
    21361 ctcaacaatg ttggcggtgt tgacggtcag ttcgaagtgc cactgtagcg cttcgttcag
    21421 aggcaacgtt ttgccctcga cagtgacagg ttcatcgcct gtcagccaca gcagttcgac
    21481 aagttctttc accagtgccg gatcgttctg ataccagacg cccagcgcgt cacccggctg
    21541 gtaacgcagg cccgagtcgc ctaagtcaat ttcgatatgg cgaacgtctt tttcagagtt
    21601 acgcccagta attttctggt taacagagag gctagccacc agcggcgcgt ctttgctgta
    21661 cgggctggtg tggatttcat ttaccgcgcc agtagcgacg gattgcgaag gtgccgcgac
    21721 aggcgcacgc gatttaagcg catcaaccac gcgggcgcgc cactcgctgg cagcagcctg
    21781 gtattcaaca tcagaatcga cacggtcgag caggcgttca ccacccagtt ccgccagctt
    21841 gctgtcaaaa tctttcccgg actggcagaa aaattcataa gagctatcgc cgaggctaaa
    21901 cacggcaaac gcggtgtttt ccagctttgg cgcttttttg gagaacagga acttatgcag
    21961 cgcgacggct tcttccggcg gttccccttc cccttgcgtt gacgtcacta cgatgagcag
    22021 tttttcgctg gcgatttgtt tgaatttata gtcgcccgcg ttcaccagct taacgttcag
    22081 ttttgctgct aataaatcat cgcgtaatgc ttcagcaacc cggcgcgcat tgccggtttg
    22141 cgaagcagag ataatcgtta tacccggcat ttctgcggcg ggcgctggcg tcactgcaag
    22201 cgcaacaggc tgctggttga gcacgcccca gaaatagcca gaaacccagg caagctgggt
    22261 aggcgttaaa tcggtcgtgg ccgcctgaag gcgtgccagt tgctccgggt tcaacggaag
    22321 caacgcggaa ggtgggacct gtgtcgtcat gcgtcgttat gttccagtaa gcaaagctgt
    22381 ttctgcgccc tgtcagcgcc cataaaacag aagagaaggt aaggttaacg gggtgaacgg
    22441 tgtggattaa agacgggata gcgataacta ataaccaaat cgactaacct gttttagcaa
    22501 tagtctttaa caacaaaata gattaaccaa cctaatgaaa aacaaatgaa tttagccaat
    22561 cattaagata aatcagcgat tttgcgcaac aaagtcgttt tagataatgc gaaaaaacag
    22621 cctttccggt actctacggc ggttttatcg tcctgtagag aaattatgat gtccaccacg
    22681 ttatttaaag atttcacctt cgaagccgct caccgcttac cacatgtccc ggaagggcat
    22741 aaatgtggtc gcctgcacgg gcattccttt atggtgcgac tggaaattac cggggaagtc
    22801 gatccgcata cgggctggat tatcgatttc gctgaactga aagcggcgtt taagccaact
    22861 tatgagcgcc tcgatcacca ttatctcaat gatattccag gtctggaaaa cccaaccagc
    22921 gaagttttag caaaatggat ttgggatcag gttaaacccg ttgtgccgct gttaagtgcg
    22981 gtgatggtaa aagaaacctg caccgcaggg tgtatctatc gcggcgaatg ataagagtgt
    23041 gtcggcggtc aatttccctt tagtaacgct atgttagggt gttgtgttct ggatatctgg
    23101 ggcatgacat ggaagacgac tgcgacatta ttattattgg tgccggtatt gcaggcaccg
    23161 cttgcgcgtt acgctgcgcg cgagcggatt tatccgtttt gttactcgaa cgcgctgaaa
    23221 tccccggcag caaaaatctt tccggcaggc ggttatatac ccatgcactc gcggaactcc
    23281 tcccgcaatt tcatctgacc gcgcctcttg aacgacgcat cactcacgaa agcctttccc
    23341 tgttaacgcc ggatggcgca acgacgtttt ccagcttaca gcccggcggt gaatcctgga
    23401 gtgtattacg tgcacggttc gatccgtggc tggttgccga agccgaaaaa gaaggtgtcg
    23461 aatgcatccc cggagcgacg gtggatgcac tgtatgaaga aaacggcaga gtctgtggcg
    23521 ttatttgtgg tgacgatatt ctccgcgccc gttatgtggt gctggcagaa ggtgccaaca
    23581 gcgtcctggc tgaacgtcac gggttagtga ctcgtcctgc tggcgaagcg atggcgttgg
    23641 ggatcaaaga agtgctgtcg ctggaaacat ccgctattga agaacgtttt catctggaga
    23701 ataacgaagg cgcagcgttg ctgttcagcg gcggaatctg tgatgactta cccggcggcg
    23761 catttcttta tactaatcaa caaacgctct cgttagggat tgtttgcccg ctctcttccc
    23821 ttacgcaaag tcgtgttccg gcaagcgagc tgctgactcg ctttaaagcg catccggcag
    23881 tgcgcacgct tatcaaaaac acggaatcac tggagtatgg tgcgcatctg gtgccagaag
    23941 gtggcttgca cagtatgccg gtgcaatacg ccggtaacgg ctggctgctg gtgggcgatg
    24001 cgttgcgcag ttgcgtcaat accggaattt ccgtgcgcgg catggatatg gcgctgactg
    24061 gcgcacaggc ggcggcacaa acgctaataa gcgcctgcca gcaccgcgag ccgcaaaatc
    24121 tgtttgcgct ttatcatcac aacgtagagc gcagcctgct atgggatgtt ctacaacgtt
    24181 atcagcatgt tccggcgctt ttgcaacgcc ctagctggta tcgggcgtgg cctgcgttaa
    24241 tgcaggatat ttcccgcgat ttatgggatc agggtgataa acctgttcca ccgctgcgcc
    24301 agttattctg gcgtcattta cgtcgtcatg gcctgtggca tctggcgggc gatgttatca
    24361 ggagtctgcg atgtctgtag cccgtaatct ctggcgcgtt gctgatgcgc cgcacattgt
    24421 tccggctgac tccgttgagc gtcagatggt acaacggttg attaacgcct gtccggtagg
    24481 tcttttttct ctcacacagg aaggtggctt acgtattgac tatcgcggct gcctggagtg
    24541 tggcacctgc cgtttgctgt gcgacgaatc cacactacaa cagtggcgct atccgccttc
    24601 cggattcggc atcacctacc gctttggata aataataagg ataatttatg cccctcttac
    24661 acctgctccg ccagaatccg gtgattgctg ccgttaaaga taatgccagc ctgcaactgg
    24721 ccatcgattc tgaatgccag tttatttccg tgttgtacgg caatatctgc accatcagta
    24781 atatcgttaa aaagattaag aacgccggga aatatgcttt tattcatgtt gatttgctgg
    24841 aaggcgcgtc aaataaagaa gtggtgattc agtttttgaa actggtgacc gaggcggatg
    24901 gaattatcag caccaaagca tcgatgctaa aagcggcaag agcggaagtt tttttctgta
    24961 ttcatcgcct gtttattgtt gattcgattt cgtttcacaa cattgataag caagttgcgc
    25021 aatcgaatcc ggattgtatt gagatcctgc caggctgcat gcccaaagtg ctgggctggg
    25081 tgacggaaaa aatccgccaa ccgctgattg ctggcgggct ggtatgcgat gaagaagatg
    25141 cgcgtaatgc gattaacgcg ggtgtcgtgg cgctttccac cacgaatacc ggagtctgga
    25201 cgttagcgaa aaaattgctt tgacgggata atcgtaacca attgaatttg ttttgatttg
    25261 ttggccgcac gccacatccg acattcagcg cctgatgcga cgcttaacgc gtcttatcag
    25321 gcctacaggt ccccagtcca gaaccgtagg caggatcatc cggcagcacg ttattggtat
    25381 tctgcgtgaa tgtttgtcac cagtgcctcc agcaccactt tccagtcatc aaccacgtca
    25441 atatctgcct gtgaaaatac cgctgcgctg gcgtcatgat taatcgccac cataaacttg
    25501 ctattgcgta cgcccgccat taacgctgct gcgccggaag cgccaacaac aatgcacacc
    25561 tcaggtgcca gcagatgtcc ggaaatacca atcacttttt cagcatcaac gccaccgttc
    25621 atcactcgcg cccgactgta gcccacttcg gttcccagct tttctgccag cataacgatt
    25681 tcctggttat ctgcctctcc gccctgcccc accaccagca cccgtctggc ctcagctaat
    25741 gggtcgcggc taacattttt cagatcctct gtgctaacca gccagtcggg aagcgcacct
    25801 gggacaatgt tcagttgctg cattccagag ggcaacgtgg cgtttttcgc tgctcccgcc
    25861 tgacgcgcca gggaaaggca taacgggcgt ttttcagttt gcagcgtcgc cgttaacgca
    25921 tttccccagt gggatttgcg catgcttacg gttggaatat ctaacgaagt cacctgacag
    25981 atgcttgcac catgtaaacg ccaggccagc cgggtcgaca gttcatcgcc aaacgttccc
    26041 ggcggaaaga gaacaacgtc cgcaggcgtt cgctgccact gttccaccat cgcatccagc
    26101 acctgttccg ccactacggg ctgaggttct atttgccagt gcgccagcgt acaaccgctg
    26161 aaatcctgcg ccgccagcca gctcgctatt gcggcattct cctgatttat tgtcacaatt
    26221 gcgatattca tggctgcatc ctttggcgta aatagtcctg ccatagcttt tgagcttttt
    26281 ctgctaccgt ttgcccatca atcagcgtcg ctccgcatcg ttgctctgct cttgccagtt
    26341 gcagacactg catcgctggc atttcggcag caacagtttt acggataatt tctgctttcc
    26401 ccgcagccat tcgctggcgc attcccggta ctggcaaggc cacttcaccg cactgacgca
    26461 cggcaatcac cgcaggcaag cgaacccggc aacagcgcaa cccatgttca gtacgctgtt
    26521 cgagggtgat aaacagcgcg tcgagagtga aacgctccac ctgggtgaag cagggccagc
    26581 ccagcatttc cgccagcaaa aatggcgttt gcccattctg cccttcgctg ctttggcagc
    26641 cggtaattat cagatccagc ggattctgac gctgccattc ggcaatatgg cgagcgacaa
    26701 attccggcgc aaagcgcaga tctgccgccg tctccagcaa tacagcttcc tcaaacccaa
    26761 gagccatgag atagcgcagc cagtgcaacg cccgttcatc ccccatgctc aacgcggtta
    26821 aagacatcgg cgtaccgttt tttctctgcg ctaacagcag cgcggcagcg gcctgttcat
    26881 cagcaccgag taaacttcgc agtagcgaaa catccggtcc gctattaccc tgagccgccg
    26941 cctgccactc tttttccgcc agcattccgg catccggttc ggctttaaac gctaacagaa
    27001 tgttcattcg cacctcctac atttcccgcc gccaccagcg ggagggcttt agtttctggt
    27061 gcccatagcc aggtcacaac aaaaccaacc aacaacactg tcgccaggag caataaggtg
    27121 acttgcattc cccactgcgc cagcacccac ggcagcaggc cagtacttac cgccgcgcca
    27181 agtcgactca tggcggtggc aaaaccgacg cccagcgagc gaatgtcagt aggaaaactt
    27241 tccgcaggca aaatgcccac cagattactg actgccgaaa tggtggtgct gaagagaaca
    27301 aaaagcagca gcgttaatga actgccggaa ggcaaacagg ccattactac cagcgttgcc
    27361 gccagcagca aaaaacttcc cagcaaaaat ttgcgatgtg ccagcaggtg cgtcagaact
    27421 aatcccagca gcgcgcccac aattaacaac gcattaagca tcaggctggc agtcagcgca
    27481 tcttccagac caatagtctg ggcgatagtt ggcagccagg tataaatcac aaaccatggg
    27541 attacgaggc agacaaagaa gacgctgtta aacgccgtgc gccgccagta acgcgaagag
    27601 aacaaggttt tgatgtgttt atgggtcgcc gttaccactt catcgcccag taaaacatgg
    27661 ggaccaaaat agcgatgcac gatagcgtga gcttctgcaa aacgcccctg gcacagtagc
    27721 caacgtggcg attctggcgt tccccagcgt aataacgtaa tcaacaacgc gggcaaagct
    27781 gccgatgcca gcaaccagcg ccaggcctcc gggttttcgg aaataaagtg atgtccggca
    27841 atacttgcca acacatagcc tacggtccac accacgctga atgcgcccag caaaataccg
    27901 cgatggcggc gcggggaaaa ttcagccagc aaggtgtgac ctactgagta atcgcctccc
    27961 agaccaatgc cgatcaaaat gcgcagccca ataagatgct ctggcgtggt ggcaaaaaat
    28021 tgcaagaacg aagcgagcgt aatcagcaaa aagctgaagg tgaagatttt ttgccgacca
    28081 atatggtcgg agatccaccc aagaaccagg ctgccaagaa acaaaccaag gagcgccgag
    28141 ccgccgatca ttcccgccat aaacggcgtc agttgcatgg cgggcgtaag ttgaataatg
    28201 gcgtaaccaa tgacgccgag aacataaccg tcggtcaggt gtgcgccgaa agtgagcgca
    28261 gcaattcgac agtgaaaacg gttaagcggt aaatcatcca ttcgcaccgg tgaagtgttc
    28321 atctttaccc cttcattgtc cggtaataag aacggcttct cgcctgagaa gccgaaaagg
    28381 caacgccccg tcatcgcgga acgctgcctg atacattatt tttcaatcgg atagatagta
    28441 cccgtattca taatgccgtt aggatcgaac tgctttttca gcccttccag caacgcccac
    28501 gcgctgccgt gttccagctt gctccagtga acgcgatgtt taccaatgcc atggtggtgc
    28561 accatcgaac cgccgaggcg aatggtttct tcgcagatga ttttgttgag cggattgtag
    28621 tacttgtcga tttcctcttc cggcttacag tcaacaacgt tgtagtcata gacgaagtac
    28681 atgttggtgc cgttctgata gctatgagag gagtgaccgc ccagcatggt gatgtcgtcg
    28741 gcgtgcggga actcagtacg aatacggtta ataacgcttt cgtagatttc gtggatgcag
    28801 ctccagcagc cggacacttc ggtggtaaag cccatgttgc cggttttgag gatctgcaca
    28861 cgttcggcag ccactttatc cggtccccag ttcaggttgt taaaccaggt ttcaatcagt
    28921 ttgctgtcca cgcgctggca ctgcgggtag cgggcaacga tttccgcaat cccttcgccc
    28981 gtcgccttcg caatgcgagg gttaccttca gccataaaga tcagcaccca ttttccgtcg
    29041 gcaaaatggg tgaagtgttg ggtgccatct tcagcgtcat acaaacgagc gatcgacgga
    29101 cgataccctt ccaccatgat ttcacgcagg atgttgaagc cggttttcat gtcttccagg
    29161 atatagccgt agaagaggtt gttttccggg gtgtatttaa agattttcac tgttacttca
    29221 gtgatatagc acaatgcacc ttcgttgccg atgatgatgt gacgaatgtc cgggccagcc
    29281 gcgcggcgtg gcacgttttt aatgcgtgtg acggtgccat ctgccaacac tgcctccaga
    29341 ccaacgacca tatcttcgat tgcgccgtag agtgtggaga actgcccgat actgcgggtt
    29401 gctaccaggc cgcccatctg cgccagcggc tttgactgcg gagaatgccc cgtggtgtaa
    29461 cctttttcac gcaacgcgtt ttccagcact tccagcggaa caccacattg caccgtcgcc
    29521 tgcatattct caatatcaat attaatgatt tgattcatgg cggagccgtc gagcaccacc
    29581 gagttttcta caacagtttc cagcccacct tcggtggcgg aagcaccggt acgcggcaca
    29641 ccgttaattt tgtgcgcatt cataaaattc agcacacggg atacttactc tgtggaaccg
    29701 agttttacga ccgctgccgg aatcggcaaa gtataaatgc catgaatatc cggaaattta
    29761 cgaaaacggt caatactgtt tttctttaat actgtttcat cggtaattac gcgatctgca
    29821 ccaacaattt cctttagctg gtcgacaatc gctgcgcgag ataaagacat agtaattcct
    29881 tcctgataat aaaaatggaa taatcattaa taagagaaaa cagaaaccac cgttatggat
    29941 tagcgcacta aataaccgcc atcgaccact aataaatgcc cgttgacata attcgatgcc
    30001 tgacttgcga gaaacacggc tgcgcccatt aaatcctgag tatcgcccca acggtttgcc
    30061 ggaatatgat caagaacgcg ctgattggtt tctggattac tgcgtgtcgc cagcataata
    30121 tgctgttgca taatagccag gggcgatacc atttacctga atattatatt gacctagttc
    30181 atcacaataa gctttggtga acccggcaag agcatgttta gtggcagaat atgcaggtga
    30241 ccattgtcca cctaagtaag agaacaatga acagatatta ataattttgc cgcttttctg
    30301 cgggatcata atttttgccg cttcatagct taactcgaat gcggcggtca ggttcacatc
    30361 aatcatcgga tcccagtcgg cacgaccgaa gtccagcacc ttattcagct tacaaatacc
    30421 ggcattgtta accagaatat cgacagtacc gaagcgctca cagcaggcag cgataatctt
    30481 ctgcggtgcg ccttctgcgg taatatccac ctgcatgaag tccacttcaa caccctgttt
    30541 ttcaatcatt tcctttgttt cgccgttatc tttgacgaag ctaggaataa aaatatttgc
    30601 gccagctttg gccaacgcca ttgcaaatgc ctgacctaaa ccgctattcc cgccggtaac
    30661 aattgctgtt ttacctttca gggagaaaaa atccattgag aacgcattga gagattcgat
    30721 tgacatagtt agctccaatt tccgtaaggc aaaaaaaaga aaggtaactt cccccgcgag
    30781 agggaagtta cctttctcat tatctctggt aactggagat aatttttagc ataacggcat
    30841 aactgctaat gtgatcgtaa tcacagtgtg atattcgtat tgtcaaatag ttttaaaaag
    30901 cagaagcgtg attactctca cacaacacct ctgctttcca tgttatttct tattgcaatt
    30961 actagagacc tgagaaaagt aatccacctg aaagggcggc ttacttttct cttttttttg
    31021 tctattacaa atgggaatac gatatgcaac acaactcata tcgccgttgg ataaccctcg
    31081 cgataattag ttttagcggc ggcgttagtt ttgacctggc ttatttacgt tatatttatc
    31141 aaattcccat ggcgaaattt atgggattca gcaataccga gataggttta ataatgagta
    31201 cctttggtat tgcggctatt attctttatg cccccagcgg cgttattgcc gataaatttt
    31261 cacaccgcaa aatgattact tccgcgatga tcattaccgg attactgggt ctgttaatgg
    31321 caacgtatcc accgctgtgg gtaatgctct gtattcaggt cgcctttgcg ataacgacga
    31381 ttttaatgct gtggtcggtg tcgattaaag ccgcctcgtt gcttggcgat catagcgagc
    31441 aagggaaaat tatgggctgg atggaagggc tgcgcggcgt cggtgtaatg tcgctggcgg
    31501 tgtttaccat gtgggtcttt tctcgctttg caccggatga cagcgccagc ctgaaaacag
    31561 tcattatcat ctacagtgtg gtttacatct tgttggggat tctgtgctgg ttttttgtta
    31621 gcgataacaa caacctgcgc agtgccaata acgaagaaaa acagtcattc cagcttagcg
    31681 acatccttgc cgttttgcgt atcagcacca cctggtattg cagcatggtg atttttggtg
    31741 tcttcacaat ctacgccatt ctgagttact ccaccaacta tctgaccgaa atgtacggca
    31801 tgtcgctggt ggcagcgagc tacatgggga ttgtgatcaa caaaatcttc cgcgcgctgt
    31861 gcggcccact tggaggcatt atcaccacct acagtaaagt gaaatcccct acccgcgtga
    31921 ttcaaatcct ttccatcatc ggtttgctgg cgttgacagc cctgctcgtc acgaactcta
    31981 acccgcaatc ggtcgcgatg gggattggcc tgattttact gctgggattc acctgttacg
    32041 cctcacacgg gctgtactgg gcctgccccg gcgaagcgag aacaccgtct tacattatgg
    32101 gcaccacggt aggtatttgt tcggtgattg gattcctgcc ggatgtcttc gtttacccga
    32161 ttatcggcca ctggcaagac accctgcccg ctgcagaagc ctaccgcaat atgtggctga
    32221 tgggcatggc ggcgcttgcc atggtgatag tctttacctt tttgctgttc caaaaaattc
    32281 gtactgctga tagcgccccc gcaatggcta gcagcaagta agccaggaaa attacaggag
    32341 aaaaatcatg tcgaaaaaat acatcatagg gattgatggc ggaagtcaga gcacaaaagt
    32401 ggtgatatac gatctggaag gtaatgtggt ttgcgaaggg aaaggcttat tgcagccgat
    32461 gcacacgcca gatgccgata ctgcagaaca tcctgacgac gatttatggg catcattatg
    32521 ttttgccggt cacgatttga tgagtcagtt tgccgggaat aaagaagata ttgtcggtat
    32581 tggtctggga tccatccgtt gctgccgtgc gttattgaaa gccgatggca cgcccgctgc
    32641 gccgttgatt agctggcagg atgcacgcgt aacgcgtccc tacgaacaca ctaatcctga
    32701 cgtggcatat gtcacctctt tttcgggtta tctgacgcat cgcttaaccg gcgagtttaa
    32761 agacaatatc gccaactatt ttggtcagtg gccggtggat tataagagct gggcatggag
    32821 cgaagatgct gcggtaatgg agaagtttaa tatcccccgt catatactgt ttgatgtgca
    32881 aatgccaggc actgttcttg ggcatatcac accacaaggc gcgctggcga cacatttccc
    32941 agctgggctg ccggttgttt gtaccaccag tgataaaccg gtagaagctc tgggggccgg
    33001 attactggat gatgaaacgg cggtgatttc actcggtact tatatcgcgc tgatgatgaa
    33061 cggcaaagcc ttgccgaaag atccggtggc gtactggccg attatgtctt ctattccgca
    33121 aacattgctg tatgaaggtt acggtattcg caaaggtatg tggacggtga gctggctgcg
    33181 cgacatgtta ggcgagtcgt taattcagga tgccagggcg caggatcttt caccggaaga
    33241 tttactcaac aagaaagcct ctagtgtgcc acctggctgt aatgggctga tgacggtgct
    33301 ggattggctg accaatccgt gggaaccgta caaacgcggg attatgatcg gctttgattc
    33361 cagcatggat tacgcatgga tatatcgttc gatactggaa agcgtggcgc tgacgctgaa
    33421 gaacaattac gacaatatgt gtaatgaaat gaatcacttt gcgaagcatg tgatcattac
    33481 tggcggcggt tcgaacagcg atctgtttat gcagattttt gccgacgtgt tcaaccttcc
    33541 ggcacgacgt aacgccatta acggttgtgc aagcctgggg gcagcgatta atacagcggt
    33601 aggtctgggg ctatacccgg attacgcaac ggctgtcgac aaaatggttc gcgtgaaaga
    33661 tatctttatg cctgttgaga gcaatgccaa acgctacgac gcgatgaata aaggtatttt
    33721 caaagaccta accaaacata ctgatgtgat cctgaaaaaa tcgtatgaag tgatgcatgg
    33781 ggaattgggg aatgcggatt cgatccagag ctggtcgaat gcgtaagtga ggaagaccgg
    33841 gcgggaaact gcccggcctg aatatacctg aatggttatc cccgctgacg cggggaactc
    33901 gaataactga ccaacgtctg gctctctaaa tccggtttat ccccgctggc gcggggaaca
    33961 ctctaaacat aacctattat taattaatga ttttttaagc cagtcacaat ctaccaactt
    34021 tacaggatca cacaaacaac acatccatta tgttaaagag cacttaatcc attgattaaa
    34081 aaggtaaata tttaaaataa ctctatacaa actaaaatct accaaacttc gccgcaataa
    34141 ttttcactcc agcaaaaaat taacgccgca gaatttgtag agaatgataa tgattagtcc
    34201 gggatgggca agatggatga gttttcaggg gcgacaaata atacaaatga gtggaatttt
    34261 ttacaagttc cacccattta taa
//
LOCUS       DAETPZ010000039        33880 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.039, whole genome shotgun sequence.
ACCESSION   DAETPZ010000039 DAETPZ010000000
VERSION     DAETPZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33880
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.039"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            535..672
                     /locus_tag="K0W59_002081"
     CDS             535..672
                     /locus_tag="K0W59_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023110.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1272..2051
                     /gene="ipaJ"
                     /locus_tag="K0W59_002082"
     CDS             1272..2051
                     /gene="ipaJ"
                     /locus_tag="K0W59_002082"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HBI3023111.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(2999..3928)
                     /gene="virB"
                     /locus_tag="K0W59_002083"
     CDS             complement(2999..3928)
                     /gene="virB"
                     /locus_tag="K0W59_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HBI3023112.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4145..4381)
                     /locus_tag="K0W59_002084"
     CDS             complement(4145..4381)
                     /locus_tag="K0W59_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBI3023113.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4385..6286)
                     /gene="ipaA"
                     /locus_tag="K0W59_002085"
     CDS             complement(4385..6286)
                     /gene="ipaA"
                     /locus_tag="K0W59_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HBI3023114.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLIDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANKINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSQIDDTSAELLTDDISDLKNNNDITAENNNIYNAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6295..7293)
                     /gene="ipaD"
                     /locus_tag="K0W59_002086"
     CDS             complement(6295..7293)
                     /gene="ipaD"
                     /locus_tag="K0W59_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HBI3023115.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNKALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRK
                     GYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7344..8435)
                     /gene="ipaC"
                     /locus_tag="K0W59_002087"
     CDS             complement(7344..8435)
                     /gene="ipaC"
                     /locus_tag="K0W59_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HBI3023116.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSI
                     SSNAVSLIISVAVLLSALRTAENKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSL
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSITQSRNSAASQIAGNIRA"
     gene            complement(8455..10197)
                     /gene="ipaB"
                     /locus_tag="K0W59_002088"
     CDS             complement(8455..10197)
                     /gene="ipaB"
                     /locus_tag="K0W59_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HBI3023117.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10203..10670)
                     /gene="ipgC"
                     /locus_tag="K0W59_002089"
     CDS             complement(10203..10670)
                     /gene="ipgC"
                     /locus_tag="K0W59_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HBI3023118.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10727..11353)
                     /gene="ipgB1"
                     /locus_tag="K0W59_002090"
     CDS             complement(10727..11353)
                     /gene="ipgB1"
                     /locus_tag="K0W59_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HBI3023119.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSHNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11369..11758)
                     /gene="ipgA"
                     /locus_tag="K0W59_002091"
     CDS             complement(11369..11758)
                     /gene="ipgA"
                     /locus_tag="K0W59_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HBI3023120.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11771..13255)
                     /gene="icsB"
                     /locus_tag="K0W59_002092"
     CDS             complement(11771..13255)
                     /gene="icsB"
                     /locus_tag="K0W59_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HBI3023121.1"
                     /translation="MSLKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGDVTKDTEINYLFEKRLGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDVVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13569..15185
                     /locus_tag="K0W59_002093"
     CDS             13569..15185
                     /locus_tag="K0W59_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HBI3023122.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVYYEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESEHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGEPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIINKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15195..15557
                     /gene="ipgE"
                     /locus_tag="K0W59_002094"
     CDS             15195..15557
                     /gene="ipgE"
                     /locus_tag="K0W59_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HBI3023123.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15557..16015
                     /gene="ipgF"
                     /locus_tag="K0W59_002095"
     CDS             15557..16015
                     /gene="ipgF"
                     /locus_tag="K0W59_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HBI3023124.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16024..17130
                     /gene="mxiG"
                     /locus_tag="K0W59_002096"
     CDS             16024..17130
                     /gene="mxiG"
                     /locus_tag="K0W59_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HBI3023125.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQSNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKKIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFFR"
     gene            17148..17399
                     /gene="mxiH"
                     /locus_tag="K0W59_002097"
     CDS             17148..17399
                     /gene="mxiH"
                     /locus_tag="K0W59_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HBI3023126.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLARNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17412..17705
                     /gene="mxiI"
                     /locus_tag="K0W59_002098"
     CDS             17412..17705
                     /gene="mxiI"
                     /locus_tag="K0W59_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HBI3023127.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17711..18436
                     /gene="mxiJ"
                     /locus_tag="K0W59_002099"
     CDS             17711..18436
                     /gene="mxiJ"
                     /locus_tag="K0W59_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HBI3023128.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18433..18960
                     /locus_tag="K0W59_002100"
     CDS             18433..18960
                     /locus_tag="K0W59_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HBI3023129.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18902..19597
                     /gene="mxiN"
                     /locus_tag="K0W59_002101"
     CDS             18902..19597
                     /gene="mxiN"
                     /locus_tag="K0W59_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HBI3023130.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALSKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19590..19997
                     /gene="mxiM"
                     /locus_tag="K0W59_002102"
     CDS             19590..19997
                     /gene="mxiM"
                     /locus_tag="K0W59_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HBI3023131.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            19981..20409
                     /gene="mxiM"
                     /locus_tag="K0W59_002103"
     CDS             19981..20409
                     /gene="mxiM"
                     /locus_tag="K0W59_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HBI3023132.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20539..21171
                     /gene="mxiE"
                     /locus_tag="K0W59_002104"
     CDS             20539..21171
                     /gene="mxiE"
                     /locus_tag="K0W59_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HBI3023133.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21158..22858
                     /gene="mxiD"
                     /locus_tag="K0W59_002105"
     CDS             21158..22858
                     /gene="mxiD"
                     /locus_tag="K0W59_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HBI3023134.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22877..23944
                     /gene="mxiC"
                     /locus_tag="K0W59_002106"
     CDS             22877..23944
                     /gene="mxiC"
                     /locus_tag="K0W59_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HBI3023135.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSQASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            23957..26017
                     /gene="mxiA"
                     /locus_tag="K0W59_002107"
                     /gene_synonym="virH"
     CDS             23957..26017
                     /gene="mxiA"
                     /locus_tag="K0W59_002107"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HBI3023136.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26030..26431
                     /gene="spa15"
                     /locus_tag="K0W59_002108"
                     /gene_synonym="spaK"
     CDS             26030..26431
                     /gene="spa15"
                     /locus_tag="K0W59_002108"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HBI3023137.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVEFHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26435..27727
                     /gene="spaL"
                     /locus_tag="K0W59_002109"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26435..27727
                     /gene="spaL"
                     /locus_tag="K0W59_002109"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HBI3023138.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27820..28158
                     /gene="spa13"
                     /locus_tag="K0W59_002110"
                     /gene_synonym="spaM"
     CDS             27820..28158
                     /gene="spa13"
                     /locus_tag="K0W59_002110"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HBI3023139.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28145..29023
                     /gene="spa32"
                     /locus_tag="K0W59_002111"
     CDS             28145..29023
                     /gene="spa32"
                     /locus_tag="K0W59_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HBI3023140.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29017..29898
                     /gene="spa33"
                     /locus_tag="K0W59_002112"
                     /gene_synonym="spaO"
     CDS             29017..29898
                     /gene="spa33"
                     /locus_tag="K0W59_002112"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HBI3023141.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29903..30553
                     /gene="spa24"
                     /locus_tag="K0W59_002113"
                     /gene_synonym="spaP"
     CDS             29903..30553
                     /gene="spa24"
                     /locus_tag="K0W59_002113"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HBI3023142.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30568..30828
                     /gene="spaQ"
                     /locus_tag="K0W59_002114"
     CDS             30568..30828
                     /gene="spaQ"
                     /locus_tag="K0W59_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HBI3023143.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30828..31598
                     /gene="spa29"
                     /locus_tag="K0W59_002115"
                     /gene_synonym="spaR"
     CDS             30828..31598
                     /gene="spa29"
                     /locus_tag="K0W59_002115"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HBI3023144.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31605..32633
                     /gene="spa40"
                     /locus_tag="K0W59_002116"
                     /gene_synonym="spaS"
     CDS             31605..32633
                     /gene="spa40"
                     /locus_tag="K0W59_002116"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HBI3023145.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32646..32888
                     /locus_tag="K0W59_002117"
     CDS             32646..32888
                     /locus_tag="K0W59_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023146.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
     gene            complement(33495..33880)
                     /locus_tag="K0W59_002118"
                     /pseudo
     CDS             complement(33495..33880)
                     /locus_tag="K0W59_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT        10540 a         4969 c         6799 g        11572 t
ORIGIN      
        1 tgacacttgt tgactacctg tgcggcatct ggtatcaagg tggcgtgaag ccggttcagg
       61 ttctcatgcg gaacgttgga acctatatcc ccctgatacc aaaggcgaaa tgtaagtgac
      121 aaaatgcaat gaacacgttc tccgttaact gaggagcatg gtgataaata aaggtaaatg
      181 cagaaggtgt agagtctcgc agatttctga catcatagag cctaacatta gatgcataac
      241 gctacagatt gtagaatctc tacaggtgaa atatacactg gttggcgttt atgtcgcaaa
      301 acctgtcatt caggttcatc tcaccaataa ggatatgagt gaagtggagg ataagtgagt
      361 ctgctgtcag agttttctgg tgtatgtcag taactgcgaa ccatgtctgc ttgatatggg
      421 agcttatgga ggctctctgt acaaggttag ggagctaaca aagcagggat aaaaagtccg
      481 cctgatgcag gcacggttcg ttaaatcttt tgccattggt aacaaaatgg tgcgatgaat
      541 gttcaggcta tctttatctt gatggtggtt cagctgtttg gtaaagaaat cgctgtaaca
      601 atagaagaac tgcaggcagt actggtccca catcgtttgc tcagtcaact ggtgaagttc
      661 cgtagcgcgt aaattaatgc tttgcatgaa acgttactgg agtggatgaa agaatacctg
      721 catatatcat tactgcgttg gattaccagt acaacagatt gaatgaactg gacatgttga
      781 tagacggcat tgaaaagcag cttataagca tagtgcggca gagttaattg gagagcactt
      841 cgccttctga gtattaagta agcatggtgg tacctgcgga tactatctat tcactgtgct
      901 cggtcggcag ccttactgac caaggagttg ggggagttga taacaaagtt gaaaaaacag
      961 ctctcaacca aggtggttat tgtaacaata gcaaataagc tggtgtgacc tgtatcacaa
     1021 taaccgatta tggacgtaaa tacgacaaaa atatgtcagt atcaggcggt attaattgct
     1081 gatgctgatg gacattaact cttaataaaa gggtggatgc tgaaaggttg ttatggtgac
     1141 ggacgtgatg acgacggaaa agagtatgag gttttggatt taaagttcgt tcggggaata
     1201 aggacccggt cagctaattc tcatagtagt tcgcattgct ggatgcaata ctgtgctgca
     1261 attactcgtc gatgtcggaa caacggaagc cttgcaaacg gggatgtatt catacaggtg
     1321 ttatgttata tggggtttta ttgcaggggg ctataccaag agaatatatg atatctcatc
     1381 agactgatgt gagggtaaat gagaatagag taaacgaaca aggttgtttt ttggcgagga
     1441 agcagatgta tgacaattct tgtggagctg ccagtttact ctgtgctgca aaggaattag
     1501 gggttgataa aataccacaa tacaaagggt ctatgtccga aatgacaagg aagagttctc
     1561 ttgatttgga taatcgttgt gagcgggatt tatacttaat aactagtggt aattataatc
     1621 cgagaataca caaagataat atcgcggatg ccggttattc tatgcctgat aagatagtca
     1681 tggctacgag gcttcttgga ttgaatgctt atgtcgttga agaaagtaac attttttcgc
     1741 aagtaattag ttttatttat cccgatgcaa gggacttatt aattggcatg ggatgtaata
     1801 tagtacatca acgagatgtg ttatcttcta accaaagagt tttggaagct gttgctgtat
     1861 cctttattgg agttcctgtc ggcctccact gggtgttgtg tagacctgat ggaagctata
     1921 tggatccagc tgtaggagaa aattattctt gtttttcaac tatggaatta ggtgcaagaa
     1981 gaagtaattc taactttata ggatatacta aaataggtat ttccatagtt ataactaatg
     2041 aggctttgta aattgtcgtt ttttatctgt gaaggagctt gaatattttg gggcgggggg
     2101 ggcataacaa atgatattac tgagtacaaa tttagctata ttgggcggat tagttgtgtg
     2161 ggaagacgaa atgtttctct tgaaataagt gaactccctc aaggaatgat tgtgtgctta
     2221 aatatttaag tgctggtaag tttgagaagg ctgacatatt ttgcagagca aacagtgatt
     2281 gacttggatg atatttttat tttagtcact cgttgtaatt ggagtatgct ttattttatt
     2341 tgaaaataga tgtattttat acccgcaatt taaactgtaa gccctatgag gggtatggat
     2401 attataagtt actctttgac taagcataac ttttggggtt agaaaaataa atctcttgtt
     2461 aataattaat atgattttaa aatcaaactt gtttataatt ataaaataat gtaagtcggt
     2521 cattttatgg gaagattagg agaataaaac agtaattata tttgagaaat ctgctaatat
     2581 attcttaaag cttttttgat actgtgaacg aagtctgtta atggtggatt ttttgtttat
     2641 caccatgtaa aaaaattgag tagtaaaagc gttgactgct aatcctcgct gtttttattg
     2701 aaacgattaa aaatacgctg aatattttcg gccagttact catcttcaca taaaattttg
     2761 accatcatgc cgcatccctt agttgcacaa ttcattttca atgatatgaa tattactaat
     2821 ttcaccagat aattttctcg tggtcagcag ggtatgagtt ttcaccgtaa ttttaaggaa
     2881 acacagtgcg tggggattga ataacagata gccgacggaa ttaccccacc ggctgaaagt
     2941 caagttcaac gaagattttt attatctgaa ttgggcagtt tacatcagtg ttcgatgttt
     3001 atgaagacga tagatggcga gaaattatat cccgaatagc ttcatctatt tctctctgaa
     3061 ctgttttgtt tattttagat aatgtaaata ttagagttct gtttgtttta ttctcttttc
     3121 tttttatgta agtccgtttg tctttagaaa taaataacga gtcaactttt agcgaagggt
     3181 tttgctttct gtttttgctt ttctttatga tgtttaaaat ttctttttta tatatgtccg
     3241 ggggcaaatt tgtatcaagg tcttttattt cttcctttaa tattggtagt gtagaactaa
     3301 gagattcatt agccttttca agtcctttat aataattgaa taatatcttg tagtcattaa
     3361 agttaagttc tgacgcgatt ggaaatagag atattatttc ctgtggaacg cttgctgcct
     3421 gaaaggcacg agtgactttc gcgcgagaca gattctcttt tttggctatg tctttatagg
     3481 acatccctga tactttcaga aaattaagac caataccaag ttctcggatg ctatgctctt
     3541 ttgctgtttg tatatcgttt gctagttttc tggcatcaag agtagatata tattcttttg
     3601 aatatagaac ttcaagatct gctcctgcat atattgcaga tgctcttcta cgagtgccat
     3661 ccagaatttc aattctacca tcaatctccc ttcctattac agggaagaat tgttgtagct
     3721 ttatagtttt tatgatatcg gctaatgatt cttctgttag ggattcttga tcgcgtacat
     3781 tcgttttttg gttgacgaag gttaaatctt ggatcctttt atgaggaatc ttggctttga
     3841 taaaggaaac tttattacca gaatggagtg taaactcttt cttttggcct tcctttatac
     3901 ttaacaagtc gttgcacaaa tccaccatat cacaccctgt ttattcatat tgatttatat
     3961 tgacaaaagt taaatgcagt gtgagcaaga ttgtatgtaa ctgttgatga tagtcaacgg
     4021 tagtcttaaa aattactatt ttaatagctg gattgatgca atttatcttt attggataaa
     4081 agaataatat tcttgtggag ctctgatgtg agagctccat agatgaaaaa atgcttatca
     4141 attactatat tgattttgta ctatttttga ctacagaaat aagatctcca atagtaataa
     4201 tgtgttctaa tgcagattca tctatacgta gattgaactc aaaactaatc atatccagca
     4261 tttcaatcag agaaagagaa tcggcagaaa gatcttcaat aaatttagtt tcttcagata
     4321 gtttgctgta cgctatacca tagcaaaagg ccactattga taatattttt tcttttatca
     4381 tattttaatc cttattgata ttctttaata cttttgatag ggaagtggtt acatcttttg
     4441 ctgcgttata tatattattg ttttcagctg taatatcatt gttatttttt aaatcagata
     4501 tatcatctgt aagtaattct gcagaggtat cgtcaatttg tgacaatacc ttgctgaggg
     4561 cgctagatac ttcttttgcc ttttcataga tcgcatgatt tttatcaatt gtatctgcgt
     4621 tggtttctgt tacgtagttt attttatttg catctgtaac ttttaatttg ttgtttattc
     4681 tgtgttctgt tgtggatttt gtaatgtcct ttgatttttc ctgtaactct cgttcttgtg
     4741 ttcctgattt agttccaatg agagatataa gattacttag ggagtttgtt actttttttg
     4801 aagcttcaaa tatcgtttcc cgtgttaaat atgtgtctcc atttcttgat aaaaaatcag
     4861 tctgaagtac ttttgaatca gtgtttgagt tgaatttatt tatttgcgaa gctactctat
     4921 gagaaagaga agcgctactt ttagttgtat ctgatgagtt atcattactg cttgtcactc
     4981 cttccgggtg gagagggggg gtggttgaag agttctgtat gttcagaggc ctgtgtcccc
     5041 gagaaagaga gttatttgca gatctttctt gactactcgt agttaacagg tctgaaccta
     5101 ttccatccga attatctgga gtatggttga aaaccttttc taaagctgaa ttatttgcaa
     5161 atgtctggac agtatctgat atagagtgtg gtagaacgga agttactttt tgaactagtg
     5221 ctctttgtgg atttagtaat gagtttccgt tacgggataa tagatcgttt gtctgagaag
     5281 taggagactg tgcatcattt tctgggttct cgtgatagct attgtcatat actctgttgt
     5341 caaaaactct attatcgtaa gttctattat cattatttat atgatagtga attacaggtt
     5401 gggatgtttt ctcattggct ccgggggttg gtccgccatc tggcatatct tcgggaggtg
     5461 tagggaggcc atcttggata ggatgcgaat cttttatatc ttctaaaata tcattaagga
     5521 cttttctcaa cagctcagga aacacggcta ctgtatttaa gtctcttgac agtttaccta
     5581 taccaaatcc tttactatca atagtatttt tacgttgatt gcgcaaacta tcaaaggcaa
     5641 gttttgcctg tgcgtcaatt aaatcatcta gtttgagaga tgaaacagtt tcattactta
     5701 actttaattc agcaagaata ttattcttga taaatgatga tacttcagta ttgaggagtt
     5761 tttgtcccaa tgataaagct tcaccagatg tgtgttgttg tgctaatagt gcaagtctgt
     5821 gctcaatgtt ttgtcgtaat tgatggagtt tcttattctt tgccgaccat ggtccaaagg
     5881 cagaatcttg ggataattta tcttttataa ttagttccga atactgtgcg acaatttttt
     5941 gaagtccctt ttcgttttca aaattaagac cattttgatg tactaaggag tacaaaacat
     6001 ttgaaaattc gagaatctcg cttttggcat acgcattcat tgagttggaa taaatgcttc
     6061 ttaacacatc ttctttccct gaggaagaaa ataaaaaatc aagacatttc tgattaaaag
     6121 acgttgcaaa gttttctttt tcagacactg atgctggtgt ttttagagaa gtttgcgaac
     6181 tatggatatc ggatattttg ctttttaact cgctgtattc tgttgatgat ggtgaagttg
     6241 ccttatataa gaatgttggc gcttgagtat tatttacatt atgcatggcg cacctcagaa
     6301 atggagaaaa agtttatctg tatctgtaca tgagcttatt gtactactca aaacctttac
     6361 taaattatca aaaatactat tggcattact gtatttttga actaaagttt gaagattatt
     6421 tttcattgtt tcatcttcgg cagagaatcc ggcattccat gcctgatatt ttgcattatc
     6481 tagcacaacc tcgccatttc cacctagatt atctaagctt tttaacatat tgtctattgg
     6541 ggtcatgttt atactgacaa catatccccc gtttttttga gataccttgc cgattgttcc
     6601 acctaattct gtaagccatt tatttgcttg ttcctgacta acagtattat ttgctggata
     6661 tagcggttta tctttatatt tttccttgag ttcttccaat gcctttttaa gcgagttgac
     6721 ttgtaatttc acggagtttc cgtcgttacc tccgggagag atccagccgg caagactgga
     6781 aagaacagcg ctaaaatctt gatacatttg agtatatgaa ctaacggcat gttcatatac
     6841 tttcagatac tgttcattaa tatcattgat ggagtttgca attttagccc acagttctct
     6901 atgagatatc atttgatctc catcaagctc ggcttctttc ggggctgaat gtaataattc
     6961 tcttgcgtct ttattaattg gatatccttt cctggaaaga atatccaata gttgagcgga
     7021 tttatttaca tccatgctaa tctgagatga atgtaatgct atttcttcta gcgatgtttt
     7081 agtcaacgtt ttttgtgaaa gctctttctt taatgcctta tttgttgttc tgatattatg
     7141 aagggtgtcg ttaagcatag taagggaact tacaggatga gaactggtcg ttgtttttat
     7201 atcagaatta actgtttcgg ttgatgaacc gttggtattg tttggactga atgatgaggt
     7261 ggaaatacta ttagtcagag ttgttatatt catggttatt tccttacagg aagagccata
     7321 tattattatg gctcttggat atattaagct cgaatgttac cagcaatctg actggctgcc
     7381 gaattccttg attgggtgat gctgtcaatt atattcaata atttttgtat taattgattt
     7441 gtcgcttggg atgcttcttt agatacttgg gatgcttcct ctgcttgttt actgctggcc
     7501 tgactgatta gttgttcttc ttcttcaaga gcagatgcat aacgccctcc tgatgtggat
     7561 atattaccag caacggctga tgtttccatt gttgcacggc caatgttttg tttttgctgc
     7621 gcagaaaggg tattgagctc gtaagttctt ctctgggtgt caattttatc ctgcaaagaa
     7681 atatcgggag aactaagaga agttttatgt tcagttgaca gggatatatt atctggcgcc
     7741 agtttatttt tacctaaaaa ttttgtgcta gagttagttt gtagtgaggt gatatttgta
     7801 tctatttgtt tatttaaccc taatttagaa cctgcaagct ctttttcaag agattgagca
     7861 gtggcaaggt tctttcttaa ggctcctttt tggtcgctaa tccctgaatg cgtttttttg
     7921 gcaccgatac ccgttatacc tacttgtgtg actgctccag taatgcttga tgatagggct
     7981 gccaggcctt gccgaacaat gttctctgca gctgattttg tagcatcgaa cgcaatcaat
     8041 gacaattgag agcccaattt attttctgca gtgcggagag cagaaagtag aacggctaca
     8101 ctaataatta aagaaacagc attagaagaa atactggaaa tatccagagt gttctctgga
     8161 gtaagggtgt gactcatctc tgaaagatct ttcatctttt tgtcagtaag gcgagcaatg
     8221 atttctgaat catgctgaac ctgctctgat aactttaaaa gttgatcatc atttaatgtg
     8281 gttgttaata cgggattttt accgacatta agtggaatat gcgctgcaat ctgctgataa
     8341 ttttgtgatt tttgtgtttc ggaagaactt tgagtttgtt ttgtggatat atctgtatat
     8401 aaaatctggg ttggttttgt gttttgaatt tccataacat tctccttatt tgtatcaagc
     8461 agtagtttgt tgcaaaattg cttttgcaac atcagttcta ttagcctgag agttggacat
     8521 tgaggctaat agatctgcaa ttacttcctg caattggccg aatttttcta ttgcttcact
     8581 gatatatttt gacagttgtt caacttgata tttcgataat gtcaggtctg ctagatttgt
     8641 cgacgcgctg ttctggaaaa cagcagaagc gacacttcct cccgcttgtg tggcagagtt
     8701 aacactttct cctaataaaa ctgcttggtt taaatgggtg gaaataattt gtttggatat
     8761 tacttcatca cccgctgcac caagaaattt atttaatctg gcaacagcat tagtgattaa
     8821 atcgtccagt tgagaagaaa aattcttgag aaactttggt ataaggtctg tgagggtttt
     8881 acctattatt ttgccaatat tctctgcaag ttttgctgct gcctgtttac caacagtggc
     8941 tacgagaacg actgctgcaa ctaggacaag agcgcctgcg attgccccca gaatagagcc
     9001 aatcattttg gcttttttcg agtcgacgcc caagccttcg agcatttttg taaatgcatc
     9061 tgaaaggagt ttgattaagg gttcaatgac tgctttcatg atcggattca gggcttgttc
     9121 catgaaggaa ttgccggtcg ctgcttgtac tatagcatcc gtaaccataa gagctaaacc
     9181 aacagctgcc agtgctagag aggctcctcc agaaaaagct gctgcaacaa cactaacgat
     9241 agttaaaagt gccccaagta ttttcccaac acaacccatt actctgttga gttcttctgc
     9301 tttacgtact tcagcagcat actcatcaga ttttctctcc atttcagttt ttcttgattc
     9361 ttggagagac tggaatagag ccagatcatt ttttaaagat tcttcattat tttttccaac
     9421 tagttgaata aaggttgcca tcaattgagt aacactggca agtccggtta atgatttctg
     9481 ctgggttgat agctgttcag cagatgctgt gtttgaaaat gcagaaaaag agtctatttc
     9541 tttttcgagt tgcattgatt tatctgtaag tttgctatga attgacaggg ttttctgctc
     9601 aatcaatgtc ctgtctttaa ctgctgcgtc ttttttgata gtgagttgta tttcttcccg
     9661 gcttaatttt ttcttttctg gtgactctgg gtcgagttcg gataatcttg tttgaatttg
     9721 gttaatttta ttttctaggt cttttatttt agaatctgcg ttttttagtt tattaatttg
     9781 tttttcatag tctctggtaa gtccttcagt ttcagataga agagtgttaa ttttatcgga
     9841 gaattctagg tttttttgct gtcttgcctg ttgctgggac ttccaagcag taattttatt
     9901 tgttaatgca gttaaagatt tttcaccgag tatttgaata aggtttccaa ttaaaagcgt
     9961 tagttgggaa cttgcattta atgactttgg tgctttaagt tcagggatta atatatttga
    10021 agttgagtgt gcattagttg tattaatatt ttggttagca gtaagatcag caattgtaag
    10081 agaaaataat ttgttagctg catcatttgc agcttggata gtattgtctc caagctcagt
    10141 ggaagccaat attttggcaa gaggaaaacc agtggttgtg gtgcttacat tatgcataat
    10201 aattactcct tgatatcctg aattgcgtcc aagtatgatt gtgcttttat ttttaatttt
    10261 tcatcattgc tgtgttgaat tacgagttcg aagcactctt tagcttttaa gggggctttc
    10321 aaccgaagct ggcattgtcc agtatggaat actggtgtat agtcattttt tcctaatgca
    10381 aaagcgacag cataaaggtc tgctgcttgt tggaactgtt cttttatctg ataaatagct
    10441 gcgagtccca taatgtagtc tacattgtaa aagtcgtata tacataaaaa tctgaagaaa
    10501 acttcagctt cctctattct tcctttgttg taaaagtcat aagcatatga ataaatgtca
    10561 tccatcatat catcaggaat tgcattaata tctttcagtg tagcgccaga gttaattgca
    10621 tcaattactg cagtagagat gctttcattt tcagtgatat ttaaagacat aaggtctcct
    10681 ttttgtctgc tttactttaa taagtataag atttaatata aaagatttaa tttgtattgc
    10741 tttgacggta tacagccttt agaacctctg cgcttatttc tatttgtaaa acttttgttg
    10801 attcattaaa tattttatcg tagacggaac tggatactag atttgtaaga tccttcattt
    10861 gtgtgttaaa caaatccatg tcactgctat agcgttttaa aatctttgct ctgaggccag
    10921 atgaggcaat ctcgtttagt gctgtatgac tgatagatgt ttgtgctgat ttttcatcaa
    10981 gttttatatc cgggaatttt ttttcaatag ctgcaaatat gttttttttg ttatctgagg
    11041 tcaaaacaac tccattgtgt atggccattt catctatact tctgtttatc attgaagaga
    11101 cataagaggt tcgctcttgg ctcatccaac aaaatagttg attccccgaa ttactatctt
    11161 ttattttgtc tatgacgctg tccctgattt tatttatatt caccgggcat ccaaaattct
    11221 tctgaggaaa gcgttgtttt aagttaaaaa cagataatat tttctttaca agcttattgt
    11281 gacttaacga atcaaatgat gggcgaggaa tcgcgaattc aacctgtgga agtattttgt
    11341 ttagaatttg catgataccc cctatatgtt agttcacttc tgaagtgatg tttgcaatgg
    11401 ttagtttaat acaaaataaa aattcataca aagattcaat gagttcatca ggtgaaagat
    11461 attttttgta gtgtttcttg gtcaaaacac attgatgagt atcagggaga agacagatag
    11521 aagaaccata atctctactc aaagagtagt taaggatgag tatgtgttct ggattaataa
    11581 tattttctgt aaaaagttca tgttcattaa gaagtccggt caaaaagatg tattcatcat
    11641 tatctgttat acaaacaggt atttgttctt cgagcaatat aaaggcttga tttttatcgt
    11701 taaaatcaag ctttgcacct gttatttcat aaagtttatc atatagtttg cgacacattt
    11761 ctatggcctt ctatatatta gaatgagagt tattcaataa aagcttatta atctgtaata
    11821 agtcgtgaat gtaaagtttg gttccaagat ctggcgattt aagagaattg taataatcaa
    11881 taatttttat tgatttttcg ataatttctt cttttttatc tgttgcttcg gtttttaatt
    11941 cgtttaatac acgagtcata ttgctgggtg ctgattttac taagtatgag tggcaagcgt
    12001 tgatgaccac atcaaagtct tctgaagata cattttcagt aggatgtttg taaagtgaaa
    12061 ttgataaagg cttgaatgtt tttggtgtgt ggataaggtt aattttattg aaacttttaa
    12121 gatagctgcg tcgtaattga gtcaatctgg aaagatcaaa atcagattca atatacttca
    12181 ttatttcgtt ataagactga ttgagacttt caatgcgttg cctaacttta tttgcatacg
    12241 cgtatacatc gttaggtgta gcaaccaatt taacgtcagg gagtggaaag tatatttcag
    12301 aatttcctgc tttaagaaca ttaagtgcca taccagcaca gttttcagat ttactgatta
    12361 atttataatt tgccaattgt ctgaaagcat ctgctttctt tttgcatata aattttgaca
    12421 tgtttgtttc atcaaggcca aaaaggttat ggctgatttt accatccttt gttttattat
    12481 cacctgataa tggaatgtat actttatcag cactgacccc ccaataacca taggggtctt
    12541 ttttttgatt agctctggga aaaaatattt ttttgttgtt attttgatct tgttttgaat
    12601 ttaataagtt tctaacctct tggcctgcat caagtctttc ggctgtttgg tctgaaatca
    12661 tatttagttt gtcttgctta taggtatcga cactgtaacc taagcgtttt tcaaaaaggt
    12721 agtttatttc tgtatctttt gtaacatccc cgccgggata ccagctgaga taggtatgag
    12781 ttggttcctg tagtaaattg ttttttatac tgatactagt atgccctaaa tgggttatgc
    12841 ctctatttcc ccagcaacga aaaactaaat gtctttcaag atttttggct gtaccgatgc
    12901 catgaaaacg ctctgcggta ttcaacatta atttgatgtc tctacctgtg aaagagtgtt
    12961 gaattgagtt tttagctgct tcataggcgg caaacgcact ttctgcagat ttttcgctag
    13021 ctaatgtatt gaggaaaagc aataaggatt tttgattctc tagtcgataa ttttttagtt
    13081 gttcatttaa tatctgtgaa tttatctttg cattaatagt gcttaatgtt ctgaaaaaga
    13141 gatcctttag gttgaatttc tgtgctatca atataggtcc atgctctgta tcttcaacgc
    13201 gtataggccc ttttgtattg cttgcgtcaa tgaaattgct aattttgagg ctcatacttt
    13261 attaactctc cattacttgg tgattgtggg gatgtaaaat gtatttggca tataatatat
    13321 tcaattaata aatttagaaa cttgagcctg ttaacataat caaattttct tttgctgtac
    13381 ataatatgta cctcgtgagc atatgtagtg ctcgtttcat catgaaatcc cacaagataa
    13441 agtgcctgat gtatcaggct cggagtgtta tagaaaaaga gagaaccctg ttgaataagt
    13501 atcttttttg ttgtaaaacg aggtacaaaa ctgcaatcaa tgccccattt atgactgagg
    13561 ataattaaat gcacataact aatttgggat tgcatcaggt ttcatttcaa agcggagatt
    13621 cctataaagg cgcagaagaa accggaaagc acaaaggtgt aagcgtgatt tcatatcaaa
    13681 gagttaaaaa tggagaaaga aataagggga tcgaagcgct taatcgatta tatttacaga
    13741 accaaacaag cttaacagga aaaagccttt tgtttgcgcg tgacagagct gaagtttatt
    13801 acgaggcaat aaagttggca ggtggtgata cgtcaaaaat taaagccatg atggaacgat
    13861 tagataccta taaacttggt gaagttaata aaagacatat taatgagctt aataaagtaa
    13921 taagtgaaga gataagagca cagctaggca ttaaaaataa aaaggaattg caaacgaaaa
    13981 taaaacaaat atttactgat tatttaaata ataagaattg gggaccggtc gacaagaata
    14041 ttagtcatca tgggaaaaat tatggttttc aattaacacc tgcctctcat atgaagatag
    14101 ggaataaaaa tatatttgtc aaagagtata atggaaaagg aatttgttgt gcttctacga
    14161 gagagagtga gcatatcgcg aatatgtggc tttcaaaagt ggtggatgac gaggggaagg
    14221 aaattttttc gggtatcaga catggagtta tatcagccta tggattgaaa aaaaactcat
    14281 ctgaaagagc tgttgctgct cgtaataaag ctgaggagtt agtaagcgca gcattatata
    14341 gcagaccaga gttattatca caggctttgt ctggtaaaac agtagattta aagattgttt
    14401 cgacttctct tctgacgccg accagtttaa ccgggggaga ggagagtatg ttaaaagatc
    14461 aggtgaacgc attaaaaggg ttaaactcta aaagggggga gccaacgaaa ttattaattc
    14521 ggaatagtga tggtcttctg aaagaagtaa gcgttaatct aaaagtggtg acatttaact
    14581 ttggtgtaaa tgaattagcg ctaaaaatgg gcttaggctg gaggaatgtt gacaagctta
    14641 atgatgaatc aatatgttct ttgttggggg ataatttcct caaaaatggt gtgattggag
    14701 gttgggctgc agaagcaata gaaaaaaatc caccatgtaa gaatgatgtg atatatctgg
    14761 ctaaccagat aaaagagatt ataaacaaga agttacaaaa aaatgataat ggagagccgt
    14821 ataaattgtc acaaagaatg actctattgg cttatactat aggtgctgta ccttgctgga
    14881 attgtaagag tgggaaggac agaacaggta tgcaggatgc tgaaattaaa agagaaataa
    14941 taagaaaaca tgaaacaggt cagttttctc aattaaatag taaattatcc tcagaagaga
    15001 aaagattatt ttctactatt ctaatgaata gtggtaatat ggaaatccaa gagatgaata
    15061 ctggtgttcc cggaaataaa gtcatgaaaa aattgccgct atcttcgtta gagctatctt
    15121 attctgaaag aataggggac ccaaaaatat ggaatatggt gaaagggtat tcgtcatttg
    15181 tataagagga atatatggaa gatttagcag atgttatttg ccgagccttg ggtatccctt
    15241 taattgatat cgatgatcaa gcaatcatgc ttgatgatga tgtgctaata tatattgaaa
    15301 aagaaggaga ttcaataaat ttattatgcc ctttttgtgc attgcctgaa aatataaatg
    15361 atcttatata tgcgttgagc ctaaattact cagaaaagat atgcttagct actgatgatg
    15421 aaggcggaaa tctaattgcg cgattagatc ttaccgggat aaatgaattc gaggatgtat
    15481 atgttaatac agaatattat atttcgcgtg tgcgttggct gaaggatgaa tttgcgcgaa
    15541 gaatgaaggg gtattaatgt cccgtttcgt atttattttg ctgtgtttta tcccatattt
    15601 agggagagct gattgttggg ataaggctgg tgaaaggtac aatattccat caagtttgtt
    15661 aaaagcgatt gcggaaaaag aatccggatt taataaatct gcagtcaatg ttaataacaa
    15721 tggaagtaaa gattatggta taatgcaaat aaatgacttt cattctaaaa gacttagaga
    15781 aatgggatat tctgaggaaa tgctaattag tcatccatgt ctttctgtac attatgcagc
    15841 aaagttatta aatgagttta tgatgatgta tggaagaggg tgggaggctg ttggagccta
    15901 taatgcagga acgtctccga aaaagaaaaa agaaagatta aagtacgcag aggatatcta
    15961 tagacgctat ttgcggatag cagcagagag caagcagaat aatcgaagga tataagagga
    16021 tttatgtctg aggcaaagaa ctcaaatctt gcaccattca ggttattagt gaaactcaca
    16081 aacggtgtcg gggatgagtt tccgttatat tatgggaata acttaattgt tctcggacgg
    16141 acgatagaaa cacttgagtt tggaaacgac aatttcccag aaaatattat tcctgtaaca
    16201 gatagtaaat cggatggtat aatttatttg accataagca aagataatat ctgtcaattt
    16261 agtgatgaaa aaggtgagca aattgatatc aattcacaat ctaattcatt tgaatatgac
    16321 gggatttctt ttcaccttaa aaatatgagg gaggataaat cgagaggcca tatactaaac
    16381 ggaatgtata agaaccactc ggtgtttttt ttctttgctg ttattgttgt gttaattata
    16441 attttttccc tctctttaaa gaaagatgaa gtaaaagaaa ttgctgaaat tatagatgat
    16501 aagagatatg gaatagtcaa cactggacag tgcaattata ttcttgccga aacacaaaat
    16561 gatgcggtat gggcgtctgt agccttaaat aaaaccggat ttaccaaatg cagatatatt
    16621 ctggtgtcaa acaaagaaat aaatagaatt caacaatata tcaatcaacg attcccattt
    16681 atcaatttgt atgtgttgaa tttagtgagt gataaagcgg aattactggt gtttttaagt
    16741 aaagaaagaa atagttcaaa agataccgaa cttgataaat tgaaaaatgc tctgattgtt
    16801 gaatttccgt atatcaaaaa tattaaattc aactatctta gcgatcataa tgcaaggggg
    16861 gatgctaaag gtattttcac aaaggtaaat gtccagtata aagagatttg tgaaaataat
    16921 aaagttacat attctgtcag agaagaactg acagatgaaa agcttgagct cattaataga
    16981 ctgatttctg aacataaaaa aatatatgga gatcagtata ttgagttttc tgtacttttg
    17041 atagatgatg attttaaagg taaatcatat cttaacagca aagacagtta tgtaatgttg
    17101 aatgataaac actggttttt ttttagataa aaataagtga ggataaaatg agtgttacag
    17161 taccgaatga tgattggaca ttgagttcat tatctgaaac ttttgatgat ggaactcaaa
    17221 cattacaagg tgaactaaca ttggcactag ataaattagc tagaaatcct tcgaatccac
    17281 agttgctggc tgaataccaa agtaaattat ctgaatatac attatatagg aacgcgcaat
    17341 ccaatacagt gaaagtgatt aaggatgttg atgctgcaat tattcaaaac ttcagataat
    17401 agggagcatt catgaattac atttatccag tcaatcaggt tgatattatc aaagccagtg
    17461 attttcaatc tcaagagata tcaagtctgg aagacgtcgt gtcggctaaa tatagtgata
    17521 ttaagatgga tacagatatt caagtatcac aaataatgga gatggtaagc aatccagaat
    17581 cattaaaccc agaatctttg gccaagttac agacgacgct ctcaaattat tcaataggag
    17641 tatcattagc tggcacgtta gcaagaaaaa cagtttcggc tgttgaaact ttattaaagt
    17701 cttaatttat atgattaggt ataaaggttt tattttattc ttgttgctga tgttgattgg
    17761 atgtgagcaa cgtgaagagt taatttctaa tttatctcaa agacaggcaa atgaaataat
    17821 atctgtgcta gaacgccata atattactgc tagaaaagtt gatggaggta aacaggggat
    17881 ctcggtacaa gtcgaaaagg ggacatttgc atcggcagtt gatttgatgc gcatgtacga
    17941 tttgccaaat ccggagagag ttgatatctc acaaatgttt cctacagatt cattagtgtc
    18001 ttctccaaga gctgaaaagg cccgtttata tagtgctatt gagcaacggc tggaacagtc
    18061 tttagtttct attggtggtg ttatttcggc aaaaatacat gttagctatg atcttgaaga
    18121 aaaaaatata tcttcaaaac cgatgcatat atcagtaatc gctatatatg actcaccgaa
    18181 agagtctgaa ctattagtta gtaatattaa gcgatttttg aaaaacacct tttctgatgt
    18241 taagtatgaa aatatatctg tcatattaac tccgaaagaa gaatatgttt atacaaatgt
    18301 acaacctgtt aaggaagtta aatcggaatt tttaacaaat gaagtaatat atttatttct
    18361 cgggatggct gtactagttg tcattctttt ggtatgggca ttcaaaacag ggtggttcaa
    18421 gagaaacaaa atatgataag aatggatgga atttataaaa aatatctttc aataattttt
    18481 gatccagcgt tctatataaa tagaaatcgg ttgaatttgc cttctgaact gttagaaaat
    18541 ggcgtaatca gaagtgagat taataatctc ataattaata aatatgatct aaattgcgat
    18601 attgaacctt taagcggggt aaccgctatg tttgttgcca actggaattt acttccagct
    18661 gttgcttatt ttatcggtag tcaagagtct cggttaatta atcatagtga gatggttatt
    18721 agttattatg ggggcaaaat cagtaagcaa ggggaggccg ctattcgaag tggtttttgg
    18781 catcttattg cgtggaagga aaatatatct gttggtatat atgaaagaat aaacttatta
    18841 tttaatccaa ttgctttgga gggaaattat acgcctgtag aaagaaattt gtcccgatta
    18901 aatgaaggta tgcaatatgc aaaaaggcac tttaccggta tccagacatc atgcctatga
    18961 tggtgttgtt ataaagagaa ttgaaaagga attatgtaaa accataaaag atagagacac
    19021 tgagagtaag aagaaagcaa tttgtgttat aaaggaggct acgaaaaaag ctgaatcact
    19081 gcgtatagat gctgtatgtg atggttatca aattggtata cagactgctt ttgagcatat
    19141 tattgactat atttgtgaat ggaagttaaa gcaaaatgaa aacagaagaa atattgagga
    19201 ctatataact agtttgcttt cagaaaattt acatgatgag aggatcatta gcactttatt
    19261 ggagcagtgg cttagttcgt tgcgtaatac agtaacagaa ttaaaagtgg ttttgcccaa
    19321 gtgtaattta gcactgagta aaaagttaga acttgatctc cataaatatc gctcagatgt
    19381 caagattatt ttaaaatatt ctgaagggaa taattatata ttctgtagtg gtaatcaagt
    19441 agtggagttt agtcctcagg atgtaatatc aggtgttaaa atagaacttg cggaaaaatt
    19501 aacaaaaaat gataaaaaat atttcaaaga attagcacat aagaaactga gacaaattgc
    19561 agaagacttg ttaaaagaga atcctgttaa tgattaatca aataaatgca agcaatgctc
    19621 tgcagcaaag attaaatagt gaagaatttg ttaatttaaa tgagagatta tcatcttcgc
    19681 aatcatttga tgaggatata atctatgaaa taatgcaata tttctcacag agtgaattaa
    19741 attccataga taatgatgaa ctgcacaata agattgaaca attattcaat tcacgttttc
    19801 cttatcttac agctgcacaa aaaagttcac ttcttaataa actcatagat gcaaatcaat
    19861 atgtcgattt gcatgaggga ttttatgcat cattgtccat atataacaat attgatttct
    19921 atattaagac tacaaccttc gattcattaa ttagtgtctt tgaagcaggg agagaggcag
    19981 atgattcgac atggtagtaa taagttgaaa atatttattt taagtatatt gctattaaca
    20041 ctgagtgggt gtgctttaaa gtcatcatct aattctgaaa aagaatggca tattgttcct
    20101 gtaagtaagg attatttttc tattccaaat gatttattat ggtcgtttaa tacaaccaat
    20161 aaaagtataa atgtttactc taaatgtatt agtggtaagg cggtttatag ttttaatgca
    20221 ggtaaattca tgggcaactt taatgttaag gaagtagatg ggtgcttcat ggatgcacaa
    20281 aagatagcta tagataaact attttctatg ctgaaagacg gggttgtttt aaaaggtaat
    20341 aagataaatg ataccatcct tatagagaag gatggggaag ttaaattaaa attaattcga
    20401 gggatataat tgtattgtga gtaaatataa aggtctaaat acaagtaata tgttttacat
    20461 ttactctagt ggacatgaac cagttaacgt tgagcttgta aaagataaag aacgtaacat
    20521 aattgagctg gctccagcat ggaagggttt ttttttgtcc gaaatcaaaa tataaaattc
    20581 agtgataatg tgaactatca ttatcgcttt aacattaata gttgtgcaaa atttcttgct
    20641 ttttgggact atttcagtgg tgctctagta gagcatagcc atgctgaaaa atgtattcat
    20701 ttctatcatg agaatgatct tcgggatagt tgtaataccg aatcgatgct tgataagtta
    20761 atgttaagat tcatcttttc atctgaccag aatgtaagta atgcactggc tatgatacga
    20821 atgactgagt catatcattt ggttctttat cttctgagaa ctattgagaa agaaaaggag
    20881 gtaagaataa agagtctgac agagcattat ggtgtgtcgg aggcttattt tagatcttta
    20941 tgtagaaaag ctttaggggc aaaagtcaaa gaacaactga acacttggcg gttagtaaat
    21001 ggtttattag atgtattttt gcacaatcaa acaatcactt ctgctgccat gaataatggg
    21061 tatgcatcaa catcccattt ttcaaatgag ataaaaacac gactaggctt ttctgcaaga
    21121 gaattatcga atattacttt tttagtgaaa aaaataaatg aaaaaattta atattaaatc
    21181 tttgactctc ttgattgtat tgttaccatt gatagttaat gctaataata ttgactctca
    21241 tttattagaa caaaatgaca tagcgaaata tgttgctcaa agtgatacgg taggatcttt
    21301 ttttgaacgt ttttctgcgt tattaaatta tcctattgtt gttagcaaac aagctgcaaa
    21361 aaagagaata tctggggagt ttgatttatc taatccagaa gaaatgcttg aaaaactgac
    21421 attacttgtg ggactcatat ggtataaaga tggaaatgca ttatacatat atgactctgg
    21481 agaattaata agcaaagtta ttttgctgga gaatatatca cttaattatc ttattcagta
    21541 tctgaaggat gctaaccttt atgatcacag atatcctata agaggaaaca ttagtgataa
    21601 aacattctat atatctggtc ctccggcatt ggttgaactg gtagctaata cagcaacttt
    21661 gcttgataag caggtttctt ctattgggac agataaagtt aattttgggg ttataaaatt
    21721 aaaaaataca tttgttagtg acagaactta taatatgaga ggggaggata ttgtcatccc
    21781 aggggtggct actgttgtag aacgtttatt aaataatggt aaggctctga gtaacagaca
    21841 agcccaaaat gatccgatgc caccctttaa tattacacag aaagtatctg aggatagtaa
    21901 tgacttttca tttagttcag ttaccaatag ttctatatta gaggatgtat cacttattgc
    21961 atatccagaa accaattcta tattagtcaa aggtaatgat cagcaaatac agattattcg
    22021 cgatattatc actcagttag atgtagccaa acgacatata gaattatcat tatggataat
    22081 cgatattgat aaatctgaat taaataacct tggtgttaat tggcaaggaa cggcttcatt
    22141 tggtgattcg tttggtgcgt catttaatat gagctcatca gcatcgataa gcactttgga
    22201 cggcaataag tttattgctt cagttatggc attaaatcag aaaaagaaag caaatgtagt
    22261 ttcaaggccg gtgattttga cacaagagaa tattccggct atatttgata ataacaggac
    22321 tttttatgtg tcattagttg gtgagcgcaa ttcttcgttg gagcatgtta cgtatggaac
    22381 attgataaat gttatacctc gcttttcatc tcggggacaa attgagatgt ctttaacgat
    22441 agaggatgga acaggaaata gtcagtcaaa ttacaattat aataatgaaa atacatctgt
    22501 ccttccagaa gttggaagga ccaaaatcag tacaatagcg agagttccac aaggcaaaag
    22561 tttgttgata ggtggttata cgcatgaaac gaactctaac gaaatagtat ctattccttt
    22621 tctctcctct attcctgtaa tcggtaatgt gttcaagtat aaaacaagta atatcagtaa
    22681 tattgtaagg gtttttctga ttcaaccccg tgagataaag gagtcaagtt attataatac
    22741 tgcagaatat aaaagcctca tatctgaaag agaaatacaa aagactacac aaataatccc
    22801 atctgaaact actttgctgg aagacgaaaa atcattggtt tcatacttaa attactaact
    22861 ataaagtagg tgatgtatgc ttgatgttaa aaatacagga gtttttagct ctgcattcat
    22921 tgataagttg aatgcaatga caaattcaga tgatggagat gagactgctg atgcagagct
    22981 tgattctggc ttggctaata gcaagtatat tgactcatct gatgagatgg cttccgctct
    23041 ttcgtcattt ataaacagaa gagaccttga gaaactgaaa ggaacaaata gtgatagtca
    23101 ggaacgtatt ttagatgggg aagaagatga aattaatcac aagatttttg atttaaagag
    23161 aacgttaaaa gataaccttc ctctagatcg ggattttata gacagactaa agagatattt
    23221 taaagatcca agtgatcaag tcttagcatt aagggaactt ttgaatgaaa aagatcttac
    23281 tgctgaacaa gtcgaattat taactaaaat tattaatgag ataatatcag gtagtgaaaa
    23341 aagtgttaat gctggaataa attcagctat acaggctaaa ttatttggca acaaaatgaa
    23401 acttgaacca cagcttttgc gtgcatgtta tcgtggtttt atcatgggga acatatcaac
    23461 aacagatcag tatatagaat ggcttggtaa ttttggtttt aatcacagac atacaattgt
    23521 gaattttgta gagcagtcac tgattgtaga catggattct gagaaaccga gctgtaatgc
    23581 ttatgagttt ggttttgtgt tatctaaatt aattgcaatt aagatgatta gaacttcaga
    23641 cgtaattttt atgaagaaac tggaatcctc aagcttgcta aaagatggca gtttaagtgc
    23701 agagcagcta ttgctaactt tattatatat ttttcaatat ccaagtgaaa gtgagcaaat
    23761 tcttacttct gttatagaag tatcacaagc cagtcatgag gattctgtag tgtatcaaac
    23821 atatctatct tctgttaatg aaagtcctca tgatatattt aaaagtgaaa gtgaaagaga
    23881 aattgcgatc aatattctac gagagcttgt cacaagtgca tacaagaaag agctttctag
    23941 ataacaggag ataaaagtga tccagtcttt tcttaagcaa gtaagtacta agcctgaatt
    24001 aattatatta gtgcttatgg ttatgatcat tgctatgttg atcataccat tacctacata
    24061 ccttgttgat tttttaatag ggcttaatat tgtacttgct attctcgttt ttatgggatc
    24121 attttatatt gaaagaatat tgagtttttc tacattccct tcggttttgt tgataacgac
    24181 attatttcgt ctcgctttat caataagtac tagtcgacta attttagttg atgcggatgc
    24241 gggtaaaatt attactacat ttggccagtt tgtcattggt gatagtctgg ctgtaggttt
    24301 tgttattttt tcaatagtaa ctgttgtgca gttcattgtt ataacaaaag gatctgaaag
    24361 ggttgcggag gtagctgctc gcttttctct tgacggtatg ccggggaaac aaatgagcat
    24421 tgatgcggat ttaaaggctg gaattattga tgccgcagga gctaaagaaa gacgcagtat
    24481 tctggaacgt gagagtcaat tgtatggttc atttgatgga gcaatgaagt tcattaaagg
    24541 tgatgcaatt gctggcatca ttattatttt tgtcaattta ataggaggta tttctgttgg
    24601 tatgagtcaa catggaatgt ccctatctgg tgcattatct acttatacca tactaactat
    24661 tggtgacgga ttggtctctc agattcctgc tctgttaatt tctataagtg caggatttat
    24721 cgttacaaga gttaatggtg atagcgataa tatgggacgt aacataatgt cccagatatt
    24781 tgggaatcct tttgttctca ttgttacatc agctcttgcc ttggcgatag ggatgttgcc
    24841 aggttttccg ttttttgttt ttttcctgat agcagttact ttgacggctt tattctatta
    24901 taaaaaggtc gtagaaaaag aaaaaagtct gtctgagtct gattctagtg gctatactgg
    24961 tacatttgat atcgataata gccatgactc atctttggca atgatagaaa atctggatgc
    25021 tattagttca gaaaccgttc ctttaatttt attatttgcc gaaaataaga taaatgcaaa
    25081 tgatatggaa ggtcttattg aaaggataag aagtcagttc tttattgatt atggtgtcag
    25141 gcttccaact attttatata gaacaagcaa tgagcttaag gtcgatgata ttgttttgtt
    25201 aataaatgag gtgcgtgccg atagttttaa tatatatttt gacaaagtgt gtattacaga
    25261 tgaaaatgga gatatagatg ctctaggtat tcctgttgtt tcaacttcat ataatgaacg
    25321 tgttatttct tgggtagacg tttcatatac agaaaatcta actaatattg atgctaaaat
    25381 taaaagtgct caagatgaat tttatcacca gttgtcacaa gctttattaa acaacataaa
    25441 tgagattttt ggtatacaag aaacaaaaaa tatgttagat cagtttgaaa atcggtatcc
    25501 tgatctatta aaggaagtct tccgacatgt gactatacag agaatttctg aggtattaca
    25561 aagattgctt ggagaaaata tttctgttcg caatttaaaa cttattatgg agtctttggc
    25621 gctttgggct cccagagaaa aagatgtcat aacattagtt gaacatgtcc gtgcatcact
    25681 ttctaggtat atttgtagta aaatagctgt ttctggtgag attaaagttg tgatgctttc
    25741 cggatatatt gaggatgcaa taagaaaagg gataaggcaa acctctggtg gctctttctt
    25801 gaatatggat atagaggttt cggatgaggt aatggaaact ttagcacatg ctttgagaga
    25861 attgagaaat gcaaaaaaaa atttcgttct tttggtatca gtagatatac gtaggtttgt
    25921 taaaagactt atagataaca gatttaagag tatactcgtt atatcgtatg ctgagattga
    25981 tgaagcatat accattaatg tattaaagac tatttagtga ggtttaaata tgagtaacat
    26041 taatttagtt caattagtta gagatagtct tttcacgatt ggttgtcctc catcaattat
    26101 tacagattta gacagccatt cagcgattac catttctttg gatagtatgc ctgctattaa
    26161 tatagctctt gtcaatgagc aggttatgtt atgggctaat tttgatgcac caagtgatgt
    26221 aaaacttcag tcatctgctt ataacatatt aaatttaatg ttaatgaact ttagttatag
    26281 cattaatgag ctggtagagt ttcatcgttc tgatgagtac ttacagttaa gagttgttat
    26341 taaagatgac tacgttcacg atgggattgt ttttgcagag attctgcatg agttttatca
    26401 gaggatggaa atcttaaatg aggtcttata atcaatgagc tatacaaaat tgctcactca
    26461 attatctttt cctaatagaa tctcggggcc aatcttggaa acaagtctta gcgatgtttc
    26521 gattggtgag atttgtaaca ttcaggctgg aattgaaagt aatgaaattg ttgcaagagc
    26581 tcaggttgta ggatttcatg atgaaaaaac aatattaagc ttgattggaa attctcgtgg
    26641 actttcacgg caaacgttga ttaagcccac tgcccagttt cttcatacgc aagtcggccg
    26701 tggattattg ggagcagtag tcaatccttt aggggaggtt actgataagt ttgctgttac
    26761 ggataacagt gaaattcttt atcgacctgt agataatgct cctccgctat atagtgaaag
    26821 ggctgcaatt gagaagcctt ttttaacagg tattaaggtt attgattctt tactcacgtg
    26881 tggtgaagga cagcgaatgg ggatttttgc gtcagctggt tgtggcaaaa cttttctcat
    26941 gaatatgctc attgaacata gtggtgctga tatatatgtt attgggttaa ttggtgagcg
    27001 aggtcgagag gttacagaaa cggttgatta tttgaaaaac tctgagaaaa aaagcaggtg
    27061 tgttttagta tatgcaactt cggattactc ttcggttgat cgttgtaatg ctgcatatat
    27121 agccactgct atagccgaat tttttaggac tgaaggacat aaagtagcgc tttttattga
    27181 ttcattaaca aggtatgcca gagcattacg tgatgtggcc ttagccgctg gagaatcacc
    27241 tgccagaaga ggctatccgg tttcggtttt tgatagctta cccagacttc ttgaaaggcc
    27301 aggaaagtta aaggcaggtg gctctattac tgcattttac actgttcttt tggaggatga
    27361 tgattttgct gatccattag ctgaagaggt aagatccatt ttagatggac atatatattt
    27421 gagcagaaat ctagcccaaa aaggacaatt tcctgcaatt gattccttaa aaagtataag
    27481 cagggtattt acacaggttg ttgatgaaaa acatcgtatt atggccgctg catttcggga
    27541 gctactttct gaaatagaag agctaaggac aattattgac tttggtgaat acaaaccggg
    27601 ggagaatgcc tctcaggata aaatctacaa caaaatatct gttgttgaga gttttctgaa
    27661 gcaagattat cgactcggtt ttacttatga gcagacaatg gagcttattg gtgaaacaat
    27721 tagataaggt tttaaaaatc aaagataaat atcaacggtc tgttaaatta attgaagcac
    27781 acatattaac actaaaaaaa aaactctttg tatcgagatg tggaggcatt agataaaagg
    27841 attatatact tcctgcaact ggagaatgac ttagaacctg taggcgctca gagcgttagt
    27901 cagcttttca atacaagacg taagatcgct atagtaaaaa agcatattat ccaatatcag
    27961 tctgaacgaa ttcttctaaa gggaagaatt gaagaaattc aaaaagatat tgatgaggca
    28021 aatgcttcta aaagaaaact gcttcataaa gagagtaaaa tatgtaaacg gattggcttg
    28081 attaaaagaa ataactttgc caaacagttg atacttgatg agttatcaca agaggatatg
    28141 aagtatggca ttagataata taaacctaaa ttttagtagt gacaaacaga tagaaaaatg
    28201 tgagaaacta tcttcaatag ataatattga tagtctcgtt ttgaagaaga agaggaaggt
    28261 tgaaattcct gagtactctt taattgcatc taactatttt actattgata agcactttga
    28321 acataagcat gataaaggag aaatttatag tggcattaaa aatgcgttcg aacttagaaa
    28381 cgaacgagcg acatattctg atattccaga atcaatggcc attaaagaaa atattttgat
    28441 accagatcaa gatatcaaag caagggaaaa aataaatatc ggcgatatga gggggatctt
    28501 ttcatataat aagagtggaa atgcagacaa gaacttcgaa agaagtcata cttcttctgt
    28561 aaaccctgat aatctgctag aatctgataa tagaaatggt caaattggtt taaaaaatca
    28621 tagcttgtct attgataaga atattgctga catcatttct ttactaaatg gaagtgttgc
    28681 taaatcattt gagctgcctg taatgaataa aaatactgca gacataaccc catccatgtc
    28741 attgcaagaa aaatcaatag ttgaaaatga taaaaatgtt tttcaaaaaa atagtgaaat
    28801 gacttaccac tttaaacagt ggggggctgg acattctgtt agtatttcag tggagtctgg
    28861 ttcttttgtt ctaaaaccgt cagatcaatt tgtaggaaat aaacttgact taattttgaa
    28921 acaagatgct gagggtaatt acagatttga tagcagtcaa cataataagg ggaataaaaa
    28981 taatagtaca ggatataatg aacagagtga agaagaatgc taagaattaa acattttgac
    29041 gctaacgaaa aactacagat tttatatgca aagcaactct gcgagcgttt ttcgattcag
    29101 acattcaaaa ataaatttac aggcagtgaa agtttagtca ctcttacttc cgtgtgtggg
    29161 gattgggtaa ttcgtattga tacattatct tttttgaaaa aaaaatacga ggtattttca
    29221 ggattttcta cacaagaatc tttactgcat ttatcaaaat gtgtctttat agagtcgtca
    29281 tctgtatttt cgattccaga actgtctgat aagattactt tccggatcac gaatgaaatc
    29341 cagtatgcaa ctactgggag tcatttatgc tgtttttcct cttctttagg tattatttat
    29401 tttgacaaga tgccggtatt acgtaatcaa gtttctcttg acttattgca tcatctttta
    29461 gagttttgct taggttcatc taatgtaagg ctggctactt taaaaagaat tcgcactggt
    29521 gatataatca tagttcagaa actttataat ttattattgt gtaatcaagt tattattggg
    29581 gattatattg tgaatgataa taatgaggca aaaattaatc tgtcagaaag taatggtgag
    29641 tcagaacaca cagaagtttc tttggcatta ttcaattatg atgatatcaa tgtaaaagtg
    29701 gactttattc ttttagaaaa aaatatgaca atcaatgaac taaaaatgta tgtagaaaac
    29761 gaattattca agtttcccga tgacatagtt aaacatgtaa atattaaagt aaatggttct
    29821 ttggttgggc atggggaact tgtttctatt gaggatggtt atggtatcga gattagttct
    29881 tggatggtaa aggagtaatc tcatgctgag tgacatgtcc ctcatagcta cactttcttt
    29941 ttttacgcta ttaccatttt tagttgctgc tggtacctgt tatataaagt tttctattgt
    30001 ttttgtaatg gtacgaaatg cacttggttt gcagcaggtt ccatcaaata tgactctcaa
    30061 tggtattgca ttgattatgg ctttgttcgt gatgaaaccc attattgaag ctggttatga
    30121 aaattatcta aatggccctc aaaaatttga tacaatatct gatattgtcc gtttttctga
    30181 ttctggcttg atggaatata agcaatactt gaaaaagcat acagatcttg agttagcacg
    30241 ttttttccaa aggtctgaag aagaaaatgc tgatttaaag agtgcagaga ataatgatta
    30301 ttcattgttt tccctcttgc ctgcatatgc gctaagtgaa atcaaagatg cgttcaaaat
    30361 aggtttttat ttatatttgc catttgttgt tgttgatctt gttatttcaa gcattttact
    30421 tgcacttggt atgatgatga tgagtccaat cacaatatca gtaccaataa aacttgtatt
    30481 atttgttgcg cttgatgggt ggggtatcct gtctaaggcc ttgattgaac aatatatcaa
    30541 tattcctgct tagatgatgg aggtaatgtg tctgatatag tttatatggg taataaggct
    30601 ctttatttaa tccttatctt ttccttatgg ccagtaggta tagctacggt tattggatta
    30661 tctattggtt tgttacagac agtgactcaa ctccaagagc agacacttcc ttttggtata
    30721 aagcttatag gtgtatcaat atctttgcta cttctttctg gatggtatgg tgaggtttta
    30781 ttgtcttttt gtcatgaaat aatgttttta attaagagtg gggtttgatg gacatttcaa
    30841 gctggttcga aagtattcat gtgtttttaa tactcctgaa cggcgttttt tttagattgg
    30901 ctccattatt tttctttctt ccatttttaa ataacggtat aatttctcca tctattagaa
    30961 tacctgtgat ttttcttgtt gcatcaggat taattacttc tggtaaggta gacataggtt
    31021 cttctgtttt tgaacatgtt tatttcctta tgttcaagga aataattgtt ggcctccttc
    31081 tctctttttg cttgtctctt cccttttgga tatttcatgc tgttggtagc attattgaca
    31141 accagcgtgg ggcaacgctt agtagttcaa ttgatcctgc caatggtgtt gatacgtctg
    31201 agttggcaaa atttttcaat cttttttctg cagttgtatt tctatacagt ggtggtatgg
    31261 tctttatttt agaatccata caattgtctt ataatatatg cccgttattt tctcaatgtt
    31321 ctttccgtgt ctcaaatatc ttaacatttc tgactttatt ggcaagtcag gctgttattt
    31381 tagccagtcc tgttatgata gtattgttac tatcagaagt attacttggt gtattatcga
    31441 gatttgctcc gcagatgaat gctttttccg tatcattaac tattaaaagt ttacttgcaa
    31501 tatttattat tttcatctgt tcttctacta tttacttttc taaagttcaa tttttcctcg
    31561 gtgaacataa atttttcaca aatctatttg ttagataaaa tattatggca aataaaacag
    31621 aaaagccgac acctaaaaaa ctaaaggatg ccgcaaaaaa aggacagtca tttaaattta
    31681 aggatttaac gactgttgtt attattctgg tagggacatt tactataata tcattctttt
    31741 ccttaagtga tgtaatgctc ttatacagat atgtaataat taatgacttc gaaattaatg
    31801 agggtaaata cttttttgca gtggttattg tcttttttaa gataattggc ttcccacttt
    31861 ttttctgtgt tctttcggct gtgttgccaa cattggttca aacaaagttt gttcttgcga
    31921 ctaaagctat caagattgat ttttcagtat taaaccctgt taaagggtta aaaaaaatat
    31981 ttagtataaa gacaataaaa gaatttttca aaagcattct gcttcttatt attctagcat
    32041 taacaaccta tttcttttgg attaatgacc gaaaaataat tttttctcag gtgttttcta
    32101 gtgttgatgg cttatatctt atttgggggg ggctgtttaa ggatataata ttatttttct
    32161 tggcattttc tatttttgtt attattcttg actttgtgat tgagttcatt ttatacatga
    32221 aagatatgat gatggataaa caggagataa aaagagaata tatagagcaa gagggacact
    32281 ttgagacaaa gtcgagaagg cgtgagttgc atatcgagat tctttcagag cagactaaat
    32341 ctgatatacg taattcaaaa ttagtggtaa tgaacccgac tcatattgca attggtattt
    32401 attttaatcc agaaatagcg cctgcacctt ttatttctct cattgaaact aaccagtgtg
    32461 ccttggctgt cagaaaatat gcaaatgaag ttggtatacc gactgtgcgt gatgtgaaat
    32521 tagctagaaa actatataaa acacatacaa aatatagttt tgttgatttt gaacacttgg
    32581 atgaagtcct acgtcttatt gtttggcttg agcaggttga aaacactcat taataagtaa
    32641 aggagatgtg ttatatggga gttaatttct gtaataaaat aggtattgat cagtctgaat
    32701 ttgaaataga atcttccatc attaactcca ttgctaatga ggtattgaac ccaatatctt
    32761 tccttagcaa taaggatata ataaatgttt tactcagaaa gatttcatct gaatgtgacc
    32821 tggttagaaa agacatttat cgttgtgctc tggagttggt cgttgaaaaa acacctgatg
    32881 atttataatc attcataata actcttttgc tgatataaat atttttgttg aagatattta
    32941 tgtagtcaca ggatcaattg tagaaaattg ttatgttttt gttaggaact gcactaaata
    33001 atatatgata agacagcaaa aacgactgac cataatcctt cttttattag gagttgataa
    33061 gagagactat tcatcctgta atgttaaaac tctgttatat agtattcgcg attatgcaaa
    33121 gtctgtaaat gatcatgaga tactaaccga gagtaatcgt ttattatctc attgcatttc
    33181 agatagtaac ggagctttct ttaaaagtag taaatacgtt ccccttaagt acttacgtaa
    33241 aaggagaata gcacgaaaga taccaaatga ttagcacttt gctagtaagc ttgctggtaa
    33301 actatattga ccgttgggtt tgttttgaga ctgtaattta aattgtgtaa ttgcctgttt
    33361 ttgatatgtt tactccaaca acggagacag gtaaattatg gacgaaaaga agcttaaagc
    33421 ccttgcggct gaactggcta aaggcctcaa aactgaggcc gacctcaatc agttttcccg
    33481 tatgctgacg aaatatcccg gctcttaaaa cgatagtggc tcaggctttc atttttcagc
    33541 gttccccaga agatttccat cggagcgttg tcgtaacagt tacctttacg cgacattgat
    33601 gttttcagac cagactgctc ctgtatgacc cggtaatcgt atgcgcagta ctgtgaacct
    33661 cgagcggagt ggtggattag cccggcaggt gggggctggc tcctgagcgc cataaacagg
    33721 gctttacccg tcaactcttt cgtcatgcgc tctcccatgg cgtaaccggc agattgtggt
    33781 cagagcttgt ggtcgccctg aacttgcgtt tctgcttaca gtgcagcctc agctccttac
    33841 gaagacgtgc cagtctgtca cgaccgacga taattccgtt
//
LOCUS       DAETPZ010000040        33048 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.040, whole genome shotgun sequence.
ACCESSION   DAETPZ010000040 DAETPZ010000000
VERSION     DAETPZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.040"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            14..4591
                     /locus_tag="K0W59_002119"
     CDS             14..4591
                     /locus_tag="K0W59_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HBI3023147.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRSSVASG
                     TDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNT
                     IALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLA
                     LGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQ
                     LYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTK
                     GKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVEANTTNITNLTDS
                     VGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKTTNDAV
                     ATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTETTSKIT
                     NVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGEDALKW
                     DKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGGNASVN
                     TDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSAKHGTNGDA
                     SVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYTIANAD
                     YDNVGDALNAIDTTPDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAISAASSD
                     AINGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALDDNALL
                     WDKTANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQNTQII
                     NQLAGNTDATYIEENGAGINYVRTNDNDLAFNDASASGVGATAVGYNAVASGASSVAI
                     GQNSSSTVDTGIALGSSSVSSRVIAKGSRDTSVTENGLVIGYDTTDGELLGALSIGDD
                     GKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTAEDSLAVGEDSLAM
                     GAKTVVNGNAGIGIGLNTLVLADAINGIAIGSNARANHANSIAMGNGSQTTRGAQTGY
                     TAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDERVAQNTQSI
                     TNLNNQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIA
                     AADNSVALGTGSVAEEENTISVGSSTNQRRITNVAASVNATDAVNVSQLKSSEAGGVR
                     YDTKADGSIDYSNITLGGGNGSTTRISNVSAGVNNNDAVNYAQLKQSAQETKQYTDQR
                     MVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSM
                     VSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            4959..6614
                     /gene="lldP"
                     /locus_tag="K0W59_002120"
     CDS             4959..6614
                     /gene="lldP"
                     /locus_tag="K0W59_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HBI3023148.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDITLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            6614..7390
                     /gene="lldR"
                     /locus_tag="K0W59_002121"
     CDS             6614..7390
                     /gene="lldR"
                     /locus_tag="K0W59_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HBI3023149.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            7387..8577
                     /gene="lldD"
                     /locus_tag="K0W59_002122"
     CDS             7387..8577
                     /gene="lldD"
                     /locus_tag="K0W59_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HBI3023150.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            8773..9246
                     /gene="trmL"
                     /locus_tag="K0W59_002123"
     CDS             8773..9246
                     /gene="trmL"
                     /locus_tag="K0W59_002123"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBI3023151.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(9299..10120)
                     /gene="cysE"
                     /locus_tag="K0W59_002124"
     CDS             complement(9299..10120)
                     /gene="cysE"
                     /locus_tag="K0W59_002124"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBI3023152.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(10200..11219)
                     /gene="gpsA"
                     /locus_tag="K0W59_002125"
     CDS             complement(10200..11219)
                     /gene="gpsA"
                     /locus_tag="K0W59_002125"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBI3023153.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(11219..11686)
                     /gene="secB"
                     /locus_tag="K0W59_002126"
     CDS             complement(11219..11686)
                     /gene="secB"
                     /locus_tag="K0W59_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HBI3023154.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(11749..12000)
                     /gene="grxC"
                     /locus_tag="K0W59_002127"
     CDS             complement(11749..12000)
                     /gene="grxC"
                     /locus_tag="K0W59_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HBI3023155.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12142..12573)
                     /locus_tag="K0W59_002128"
     CDS             complement(12142..12573)
                     /locus_tag="K0W59_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBI3023156.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            12818..14362
                     /gene="gpmM"
                     /locus_tag="K0W59_002129"
                     /gene_synonym="pgmI"
     CDS             12818..14362
                     /gene="gpmM"
                     /locus_tag="K0W59_002129"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBI3023157.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            14372..15655
                     /gene="envC"
                     /locus_tag="K0W59_002130"
     CDS             14372..15655
                     /gene="envC"
                     /locus_tag="K0W59_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HBI3023158.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKEHAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQVLNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            15659..16618
                     /locus_tag="K0W59_002131"
     CDS             15659..16618
                     /locus_tag="K0W59_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HBI3023159.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQHEAAKFNASAN"
     gene            complement(16605..17639)
                     /gene="waaH"
                     /locus_tag="K0W59_002132"
     CDS             complement(16605..17639)
                     /gene="waaH"
                     /locus_tag="K0W59_002132"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HBI3023160.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(17878..18903)
                     /gene="tdh"
                     /locus_tag="K0W59_002133"
     CDS             complement(17878..18903)
                     /gene="tdh"
                     /locus_tag="K0W59_002133"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBI3023161.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(18913..20109)
                     /gene="kbl"
                     /locus_tag="K0W59_002134"
     CDS             complement(18913..20109)
                     /gene="kbl"
                     /locus_tag="K0W59_002134"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBI3023162.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKCYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(20384..21241)
                     /gene="yibB"
                     /locus_tag="K0W59_002135"
     CDS             complement(20384..21241)
                     /gene="yibB"
                     /locus_tag="K0W59_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HBI3023163.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            21546..22478
                     /gene="rfaD"
                     /locus_tag="K0W59_002136"
     CDS             21546..22478
                     /gene="rfaD"
                     /locus_tag="K0W59_002136"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HBI3023164.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            22488..23534
                     /gene="rfaF"
                     /locus_tag="K0W59_002137"
     CDS             22488..23534
                     /gene="rfaF"
                     /locus_tag="K0W59_002137"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HBI3023165.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            23538..24530
                     /gene="rfaC"
                     /locus_tag="K0W59_002138"
     CDS             23538..24530
                     /gene="rfaC"
                     /locus_tag="K0W59_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HBI3023166.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLDYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            24527..25735
                     /gene="rfaL"
                     /locus_tag="K0W59_002139"
     CDS             24527..25735
                     /gene="rfaL"
                     /locus_tag="K0W59_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HBI3023167.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(25771..26913)
                     /locus_tag="K0W59_002140"
     CDS             complement(25771..26913)
                     /locus_tag="K0W59_002140"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HBI3023168.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(26922..27935)
                     /locus_tag="K0W59_002141"
     CDS             complement(26922..27935)
                     /locus_tag="K0W59_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HBI3023169.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(27960..28667)
                     /gene="rfaY"
                     /locus_tag="K0W59_002142"
     CDS             complement(27960..28667)
                     /gene="rfaY"
                     /locus_tag="K0W59_002142"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HBI3023170.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(28693..29700)
                     /gene="waaO"
                     /locus_tag="K0W59_002143"
                     /gene_synonym="rfaI"
     CDS             complement(28693..29700)
                     /gene="waaO"
                     /locus_tag="K0W59_002143"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HBI3023171.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(29743..30540)
                     /gene="rfaP"
                     /locus_tag="K0W59_002144"
     CDS             complement(29743..30540)
                     /gene="rfaP"
                     /locus_tag="K0W59_002144"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HBI3023172.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(30533..31657)
                     /locus_tag="K0W59_002145"
     CDS             complement(30533..31657)
                     /locus_tag="K0W59_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBI3023173.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(31654..32712)
                     /gene="rfaQ"
                     /locus_tag="K0W59_002146"
     CDS             complement(31654..32712)
                     /gene="rfaQ"
                     /locus_tag="K0W59_002146"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HBI3023174.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         8734 a         8086 c         7801 g         8427 t
ORIGIN      
        1 gcgagcatca ttaatgaaca aaatatttaa agttatctgg aaccctgcga cagggaatta
       61 tactgttacc agcgaaacgg caaaaagccg tggcaagaaa tctgggcgca gtaagctgtt
      121 aatttctgcg ctggttgcgg gtggaatgtt gtcgtcgttt ggggcattgg cgaatgccgg
      181 gaatgacaac ggtcagggtg ttgattacgg tagtggatca gctggcgacg gctgggttgc
      241 tataggcaaa ggggcgaaag caaatacttt tatgaacacc agtggttcca gtactgctgt
      301 gggttatgac gctatagctg aaggccaata tagctctgcc atcgggtcaa aaacccatgc
      361 gattggtggt gcatcaatgg cctttggggt tagtgcaata tcagaaggcg atagaagtat
      421 agcactgggt gcctcttcgt attcattggg ccaatactca atggccctgg ggcgtagcag
      481 tgtagctagc ggtactgaca gcctcgcatt tggcagacaa tcacttgcca gcgcagcgaa
      541 cgctattgcg ataggtgctg agaccgaagc cgctgaaaat gcaactgcta ttggcaataa
      601 tgcgaaggca aaagggacta atagcatggc aatggggttc ggaagccttg ccgataaagt
      661 caatactatc gcattaggaa atggcagcca ggctctggca gataatgcaa tcgccatagg
      721 ccagggcaac aaagctgatg gcgtggatgc catcgctctg ggtaatggta gccagtcgag
      781 aggcttaaac accattgcct taggcacagc cagtaatgca actggtgata agagtcttgc
      841 gcttggtagt aatagcagtg ccaacggtat taactctgtc gcgctgggcg cagattccat
      901 tgcggattta gacaataccg tctctgtcgg caatagttca ttaaaacgca agatcgttaa
      961 tgtgaaaaat ggcgcgatca agtctgacag ttacgatgcc attaatggtt cacagcttta
     1021 tgccattagc gactcggtag caaaaaggct tggaggaggg gctgcagtag atgttgatga
     1081 cggtactgtt acagcaccaa cctacaattt aaaaaatggt agcaaaaata acgtaggggc
     1141 tgcgctcgct gtacttgatg aaaacaccct gcaatgggac caaaccaaag gcaaatacag
     1201 cgctgctcat ggtactagta gcccaactgc cagcgtaatc accgatgttg cggatggcac
     1261 gatttcagcc tccagtaagg atgcggttaa cggttcccaa ctgaaagcta ccaatgacga
     1321 tgtcgaagcc aacaccacca atatcaccaa cctgacggat tccgttggtg accttcaggc
     1381 tgatgccctg ctctggaacg aaactaaaaa ggcattcagt gcagctcacg gccaggatac
     1441 caccagcaaa ataaccaacg ttaaagatgc cgacctgacg gctgacagca ctgatgctgt
     1501 taacggctct cagctgaaaa ccaccaacga tgctgtggcg acgaatacca ccaatatcgc
     1561 caataacact tccaatattg ccactaacac caccaacatc tctaacctga ctgagacggt
     1621 gactaatctt ggtgaggatg cgctgaaatg ggataaggac aatggtgtat tcacggcagc
     1681 tcatggcacc gagaccacca gcaaaataac caacgttaaa gatggcgacc tgacgactgg
     1741 cagcaccgat gccgttaacg gctctcagct gaaaaccacc aacgatgccg tggcgacgaa
     1801 taccaccaat atcgccacta acaccaccaa catctctaat ctgactgaga cggtgactaa
     1861 tcttggtgag gatgcgctga aatgggataa ggacaatggt gtcttcactg cagctcatgg
     1921 caacaatacc gccagcaaaa tcaccaatat cctggacggc acagtcactg caaccagttc
     1981 cgatgccatt aacggtagcc agctttatga cttaagcagc aatatcgcca cctacttcgg
     2041 cggcaatgct tctgtgaata ctgacggcgt gtttaccggt ccaacctaca aaatcggtga
     2101 aacaaattat tataacgtcg gcgatgcact ggctgcgatt aactcctcat ttagcacgtc
     2161 tctcggcgat gctctgcttt gggatgccac cgcaggtaaa ttcagtgcca aacacggtac
     2221 taatggtgac gcaagcgtga tcactgatgt cgcagatggt gaaatttcag actccagttc
     2281 tgacgcagta aacggctcac aactccacgg cgtgagcagt tatgttgttg atgcgctggg
     2341 gggtggtgcc gaagtcaatg cagacggcac catcactgcg ccgacgtaca ccattgctaa
     2401 tgctgattac gataatgtcg gtgatgccct gaatgctatc gataccactc ctgacgacgc
     2461 tctgctctgg gatgcggacg ccggtgaaaa tggtgcattt agcgccgctc acggaaaaga
     2521 taaaactgcc agtgtaatca ctaacgtcgc taacggtgca atctctgctg ccagcagcga
     2581 cgcgattaac ggctcacaac tctataccac caataagtac atcgctgatg cgctgggtgg
     2641 tgacgcagaa gtcaacgctg acggcaccat caccgcaccg acttacacca ttgcgaacgc
     2701 cgagtacaac aacgtcggtg acgccctgga tgcgcttgat gataacgccc tgctgtggga
     2761 taagactgcc aatggcggtg ctggagccta caatgccagc catgacggta aagccagcat
     2821 catcactaat gtcgctaatg gcagtattag tgaggacagt accgatgcag tgaacggttc
     2881 tcagttgaat gcgacgaata tgatgattga gcagaacacc caaattatca atcagctcgc
     2941 tggtaacacc gacgcaacct atatcgaaga aaatggcgct ggtatcaact acgttcgtac
     3001 taacgacaac gatttagcct ttaacgatgc aagcgcctct ggtgtcggcg ctacagctgt
     3061 aggttataac gctgtcgcgt ctggtgccag cagcgtagcc attggtcaga acagcagcag
     3121 caccgttgat accggtattg cgctgggtag cagctccgtt tccagccgtg tgattgccaa
     3181 aggttctcgt gacactagcg taacggaaaa tggcttggtg attggttatg acaccactga
     3241 cggcgaactg ctaggtgcat tgtcaattgg tgatgacggt aaataccgcc aaatcatcaa
     3301 cgtagctgat ggttcagaag cccatgacgc cgttacggtt cgccagttgc agaacgctat
     3361 tggagcggtc gccactacgc caaccaagta ctttcacgcc aactcaacgg cagaagactc
     3421 actggccgtt ggtgaagact cgctggcaat gggtgcgaaa actgtcgtta atggtaatgc
     3481 aggtattggt atcggtttga acactctggt tctggctgat gcgatcaacg gcattgctat
     3541 cggcagtaac gcacgcgcaa accatgcaaa cagtatcgca atgggtaacg gttctcagac
     3601 cacccgtggt gcacagactg gctacaccgc ctacaacatg gacgcaccgc agaactctgt
     3661 aggtgagttt tctgtcggta gtgaagacgg tcaacgtcag atcacaaacg tcgcagctgg
     3721 ttcagcggat accgatgcgg ttaacgtggg tcagttgaaa gtcactgatg agcgcgtagc
     3781 gcaaaatacc cagagcatta ctaacctgaa caatcaggtc actaatctgg atactcgcgt
     3841 tactaatatc gaaaacggta ttggcgacat tgtcaccacc ggtagcacca agtacttcaa
     3901 gaccaacacc gatggcgtag atgccaacgc ccagggtaaa gatagcgttg ctattggttc
     3961 tggttccatt gctgccgctg acaacagcgt cgcactgggt accggttccg ttgcagagga
     4021 agaaaataca atctctgtag gttcttctac taaccaacgc cgtatcacca acgtagctgc
     4081 cagtgttaat gccaccgatg cggttaacgt gtcgcagctg aaatcttctg aagcaggcgg
     4141 agtgcgctac gacaccaaag ctgatggttc tatcgactat agcaatatca ccctcggtgg
     4201 tggcaacggc agtacgactc gtatcagcaa cgtctccgct ggcgtcaaca acaacgacgc
     4261 ggtgaactac gcgcagttga agcaaagcgc gcaggaaacg aagcaataca ccgatcagcg
     4321 gatggttgag atggataaca aactgtctaa aactgaaagc aagttgagcg gtggtatcgc
     4381 ttctgcaatg gcaatgaccg gtctgccgca ggcttataca ccgggtgcca gcatggcttc
     4441 tattggtggc ggtacttaca acggtgaatc ggcagttgct ttaggtgtat cgatggtgag
     4501 cgccaatggt cgttgggtct acaaattaca aggtagtacc aatagccagg gtgaatactc
     4561 cgccgcactc ggtgccggta ttcagtggta atgatccatt aagttagtgt gactaagggc
     4621 aaacgccaaa ttgcctgatg cgttacgcgc atcaggcaag ataaggcgct ccgggtcaac
     4681 aatctaaggg caattctctg atgaggattg cccttttctt taccagacat ctccccccac
     4741 aagaattggc cctaccaatt cttcgcttat ctgacctctg gttcacaatt ccccaattaa
     4801 aactcacatc aatattgcta atacataaca tttagttaac cattcattgt cattatccct
     4861 acacaacaca attggcagtg ccacttttac acaacgtgtg acaaggagat gagcaacaga
     4921 ctcattacac gatgtgcgtg gactctagga gacctgcaat gaatctctgg caacaaaact
     4981 acgatcccgc cgggaatatc tggctttcca gtctgatagc atcgcttccc atcctgtttt
     5041 tcttctttgc gctgattaag ctcaaactga aaggatacgt cgccgcctca tggacggtag
     5101 caatcgccct tgccgtggct ttgctgttct ataaaatgcc ggtcgctaac gcgctggcct
     5161 cggtggttta tggcttcttc tatggtttgt ggcccatcgc gtggatcatt attgcagcag
     5221 tgttcgtcta taagatctcg gtgaaaaccg ggcagtttga catcattcgc tcgtctattc
     5281 tttcgataac ccctgaccag cgtctgcaaa tgctgatcgt cggtttctgt ttcggcgctt
     5341 tccttgaagg agccgcaggc tttggcgcac cggtagcaat taccgccgca ttgctggtcg
     5401 gcctgggttt taaaccgctg tacgccgccg gactgtgcct gattgttaac accgcgccag
     5461 tggcatttgg cgcgatgggc attccaatcc tggttgccgg acaagtaaca ggcatcgaca
     5521 gctttgagat tggccagatg gtggggcgtc agctaccgtt tatgaccatt atcgtgctgt
     5581 tctggatcat ggcgattatg gacggctggc gcggtatcaa agagacgtgg cctgcggtcg
     5641 tggttgcagg cggctcgttt gccatcgctc agtaccttag ctctaacttc attgggccgg
     5701 agctgccgga cattatctct tcgctggtat cactgctctg cctgacgctg ttcctcaaac
     5761 gctggcaacc agttcgcgta ttccgcttcg gtgacttggg ggcgtcacag gttgatataa
     5821 cgctggccca caccggttac accgcgggtc aggtgttacg tgcctggaca ccgttcctgt
     5881 tcctgaccgc taccgtaaca ctgtggagca tcccgccgtt taaagccctg ttcgcatcgg
     5941 gtggtgcgct gtatgagtgg gtgatcaaca ttccggtgcc gtacctcaat aaactggttg
     6001 cccgtatgcc accagtggtc agcgaggcta cagcctatgc cgccgtgttt aagtttgact
     6061 ggttctctgc caccggcacc gccattctgt ttgctgcact gctctcgatt gtctggctga
     6121 agatgaaacc gtctgacgct atcagcacct tcggcagcac gctgaaagaa ctggctctac
     6181 ccatctactc catcggcatg gtgctggcgt tcgcctttat ctcgaactat tccggactgt
     6241 catcaacact ggcactggca ctggcgcaca ccggtcatgc attcaccttc ttctcgccgt
     6301 tcctcggctg gctgggggta ttcctgaccg ggtcggatac ctcatctaac gccctgttcg
     6361 ccgcgctgca agccaccgca gcacaacaaa ttggcgtctc tgatctgttg ctggttgccg
     6421 ccaataccac cggtggcgtc accggtaaga tgatctcccc gcaatctatc gctatcgcct
     6481 gtgcggcggt aggcctggtg ggcaaagagt ctgatttgtt ccgctttact gtcaaacaca
     6541 gcctgatctt cacctgtatg gtgggcgtga tcaccacgct tcaggcttat gtcttaacgt
     6601 ggatgattcc ttaatgattg ttttacccag acgcctgtca gacgaggttg ccgatcgtgt
     6661 gcgggcgctg attgatgaaa aaaacctgga agcgggcatg aagttgcccg ctgagcgcca
     6721 actggcgatg caactcggcg tatcacgtaa ttcactacgc gaggcgctgg caaaactggt
     6781 gagcgaaggc gtgctgctca gtcgacgcgg cggcgggacg tttattcgct ggcgtcatga
     6841 cacatggtcg gagcaaaaca tcgtccagcc gctaaaaacg ctgatggccg atgatccgga
     6901 ttacagtttc gatattctgg aagcccgcta cgccattgaa gccagcaccg catggcatgc
     6961 ggcaatgcgc gccacacctg gcgacaaaga aaagattcag ctttgctttg aagcaacgct
     7021 aagtgaagac ccggatatcg cctcacaagc ggacgttcgt tttcatctgg cgattgccga
     7081 agcctcacat aacatcgtgc tgctgcaaac catgcgcggt ttcttcgatg tcctgcaatc
     7141 ctcagtgaag catagccgtc agcggatgta tctggtgcca ccggtttttt cacaactgac
     7201 cgaacaacat caggctgtca ttgacgccat ttttgctggt gatgccgacg gggcgcgtaa
     7261 agcaatgatg gcgcacctta gttttgttca caccaccatg aaacgattcg atgaagatca
     7321 ggctcgccac gcacggatta cccgcctgcc cggtgagcat aatgagcatt cgagggagaa
     7381 aaacgcatga ttatttccgc agccagcgat tatcgcgccg cagcgcaacg cattctgccg
     7441 ccgttcctgt tccactatat ggatggtggt gcatattctg aatacacgct gcgccgcaac
     7501 gtggaagatt tgtcagaagt ggcactgcgc cagcgtattc tgaaaaacat gtccgactta
     7561 agcctggaaa cgacgctgtt taatgagaaa ttgtcgatgc cggtagcact ggcaccggtg
     7621 ggtttgtgtg gcatgtatgc gcgtcgtggc gaagttcagg cagccaaagc ggcggacgcg
     7681 catggcattc cgtttactct ctcgacggtt tccgtttgcc cgattgaaga agtcgcgcca
     7741 gccatcaagc gcccaatgtg gttccagctt tatgtactgc gcgatcgcgg ctttatgcgt
     7801 aacgcgctgg agcgagcaaa agcagcgggt tgttcgacgc tggttttcac cgtggatatg
     7861 ccgacaccgg gcgcacgcta ccgtgatgcg cattcaggta tgagcggccc aaatgcggca
     7921 atgcgccgct acttgcaggc ggtgacgcat ccgcaatggg cgtgggatgt gggcctgaac
     7981 ggtcgtccac atgatttagg taatatctca gcttatctcg gcaaaccgac cggactggaa
     8041 gattacatcg gctggctggg gaataacttc gatccgtcca tctcatggaa agaccttgag
     8101 tggatccgcg atttctggga tggcccgatg gtgatcaaag ggatcctcga tccggaagat
     8161 gcgcgcgatg cagtacgttt tggtgctgat ggaattgtgg tttctaacca cggtggccgc
     8221 cagctggacg gtgtactctc ttccgctcgt gcgttgcccg ctattgcaga tgcggtgaaa
     8281 ggtgatatcg ccattctggc ggatagcgga attcgtaacg ggcttgatgt cgtgcgtatg
     8341 attgcgctcg gtgccgacac cgtactgctg ggtcgtgctt tcctgtatgc gctggcaaca
     8401 gcgggccagg cgggtgtagc taacctgcta aatctgatcg aaaaagagat gaaagtggcg
     8461 atgacgctga ctggcgcgaa atcgatcagt gaaattacgc aagattcgct ggtgcagggg
     8521 ctgggtaaag agttgcctac ggcactggct ccgatggcga aagggaatgc ggcatagtcg
     8581 tttgcgcccc ctcaccctaa ccctctccct cagggagagg ggaccgttcg gcgctgtatg
     8641 tactccctca ctctgaaacg acaccgcact ctttttttct ccctcgcccc tccggggagg
     8701 gccggggtga ggggaaaagg ccgcacggtt caccaatttt ctgctatcct gcccccgcac
     8761 taagggggca gtatgctaaa catcgtactt tacgaaccag aaattccgcc aaatactggc
     8821 aacatcatcc gtctttgcgc taataccggc tttcgtctac atatcatcga accgatggga
     8881 tttgcctggg acgacaagcg cctgcgccgc gcggggctgg actatcacga gtttaccgcc
     8941 gttacgcgtc atcatgacta tcgcgcgttc ctcgaagcag aaaatcccca gcgcctgttt
     9001 gccctcacca cgaaaggtac gcctgctcac agcgccgtaa gctatcagga tggcgattat
     9061 ctgatgttcg gcccggaaac acgcggcctg ccagcgagca ttcttgatgc cctgcccgct
     9121 gaacaaaaaa ttcgcattcc gatggtgccg gacagccgca gcatgaatct gtccaatgcg
     9181 gtgtcggtag tggtgtatga agcctggcgg cagctgggat atccgggagc gttgttgaga
     9241 gattagttac tgtatgccgg atagatgatt acatcgcatc cggcacgatc acaggacatt
     9301 agatcccatc cccatactca aatgtatggt taataccgtt gaaatgctgg tccatatcca
     9361 ttgatggctt atcgctgtct ggtttaccga caatacgagc cggaacgcca gcggcggttg
     9421 tatgcggcgg caccggttgc agcaccacgg aacctgcgcc aatcttcgcg ccgcgaccaa
     9481 cttcaatatt gccgaggatt ttcgcgcccg cgccaatcat cacaccttca cgaattttcg
     9541 ggtgacggtc accaccagat ttacccgtac cgccaagcgt cacagattgc agaatcgata
     9601 cgtcgttttc aattaccgcc gtttcaccca caacgatgcc cgtggcgtgg tcgagcataa
     9661 tgccacggcc aatttttgct gccgggtgaa tatcgacctg gaacgtcaca gaaacctggt
     9721 tttgcagaaa gattgccaat gcgcgacgcc cctgattcca caaccagtga ccgatgcgat
     9781 aggcctgcaa ggcatgaaaa cccttcaggt ataacaacgg ggttgagtat ttatcgactg
     9841 ccgggtcgcg ggtacgcacc gcctgaatat cacaggccgc agaggcgatc atttccgggt
     9901 cagcggcgta ggcttcttca accacttcac ggatagcaat agcaggcata attggcgatg
     9961 acagcttgtt cgccagcatg tagctcagtg cactgccaag gttttcgtgc ttgagtagcg
    10021 tcgcgtggta aaaactggcc agcattggct cacagtccgc cagcgttctg gcttcggctt
    10081 taatattgtt ccagacaatt tccagttctt cacacgacat tgcttactcc acacgatgag
    10141 ataatgaccg gcccgttctg cgcgggccgg gtcatagcgg taacaaaggt tccctggggt
    10201 tagtggctgc tgcgctcgtc cttgcgtgca cgacctaata atgtcaatgc tgcctcgcgc
    10261 gcgttttttc cgcaatataa tacttgataa atttcctcgg ttattggcat ttcaacgccg
    10321 aagcgatgcg ccagttcgcg gacttctttc gtattgcggt agccttccac cacctgacca
    10381 atcttctcct gcgcgctttg tacatccatg ccctgaccga gcatcatgcc aaaacggcgg
    10441 ttacgcgact ggttgtcggt acaggtaagc accagatcgc ccagccccgc catgcccata
    10501 aaggtggcag gatcggcacc cagcgccgca ccaagacgcg acatttcagc cagcccacgg
    10561 gtgatcagcg ccgtacgcgc attcgcacca aaaccgatac cgtcagacat ccccgcgcca
    10621 atggcaatga cgtttttcac cgcgccgcca agctgcacgc caatgaaatc cgggttgctg
    10681 taaacgcgga aacttttgcc gcagtgcagc agctgctgga gatcatcggc aaaggtctga
    10741 tcggtcgagg ccagcgaaat agctgtcggt aaacctgccg ccagttcttt cgcaaacgtt
    10801 gggccagaga taaccgccag cggaatttga tcgcctaagg cctcacgcgc cacgtcctgt
    10861 aacagacgtc cggtttccgc ttccagccct ttggtcgccc acaccagacg cgcatcagga
    10921 cgcatcagcg gtttaatctg gcgcagcact tcaccaaaga catggctggg tacgacgacg
    10981 agaatattac gactggctgc cagcgcagtg gcgagatcgc tttcaagatg gagcgtatcg
    11041 ggaaaaggca catcggggag aaacgcggcg ttacagcggt cgcgttcaag cgttgcgata
    11101 tgttcagggt catggcccca gaggacaacc tcgtggccat ttcttgccag ggtaatggca
    11161 agagcggtgc cgtacgagcc ggcaccgatc acagtcattg aagcattacg ttggttcatc
    11221 aggcatcctg atgttcttca gtaccttcgc cagcctgctg ctgcaaatag ttcatgaaca
    11281 gcgcatcgaa gttaaccggc gcaaggttca gttgcgggaa tgtaccgcgg gataccatgc
    11341 tggtgatgca ctcacgagca tacgggaaca gaatgttcgg gcagtatgcg cccaggcaat
    11401 gtgccatctg ggtgccttcg atacccgcga tggagaaaat accgccctgc tgaacttcac
    11461 acaggaacgc ggtttcttcg cccaaggagg ccgttacggt aacacgcagt accacttcgt
    11521 atacgtcatc tgccagttgg gaagatgccg tatccagatc aagtttaact tctggttgcc
    11581 aatctttctg gaaaacgtgc ggcgcgttcg gcgcttcgaa agagatatcc ttggtataaa
    11641 tacgttggat ctggaaagtc atttcagtgt tgttttgttc tgacatgtgt agaaaaccct
    11701 taagtgttgt ccttaaatac tgcgcaatgt aatgccaacg ttcacacgtt atttcagcag
    11761 gggatccagt ccaccacgtg catccaatgc atacaagtca tcacagccgc caatgtgctg
    11821 tgcgtcaata aaaatctggg gaaccgtggt gcgaccgctg cgtttgatca tctcttcacg
    11881 cttggcggca ttgccatcga tcggcaactc ctggaaactc acgcccttgc tgctcagcag
    11941 tgcttttgca cgatggcaat acgggcaggt ttctttggta tagatttcaa cattggccat
    12001 aatttagctc ctgttttttt accctgaaat ttcatgttgc agggcggctg caaaatggag
    12061 tgtccccaga agcttataac aataagtcgc tgggggccta attgcagcta acggcctgca
    12121 tcatgaaaga cgacaggtaa attatttgcc gcgcaccaga ggcaagtttt cgccagccca
    12181 gccagcaacg ccctctttca gcacgaatac ttgcgcaaaa ccagctttcg tcagcgcgtt
    12241 tgcaggctcc tggcactgca tgccagaacc gtctaccacg ataaccgatt tgtctttgtg
    12301 cttctcaagc tcaccaacat tgttggcttt gatttcgctc ggcaacagat tgatagaacc
    12361 tgcgatatgg cctttacgga agtcatcacg ctgacgtaaa tccacaacca cagcgtcttc
    12421 tttgttgatc agacgcgtag cttcaccacg agtaatcacc ttcactttcg aggtcaggct
    12481 cttaaacgta gtcacaagaa ccgccaccag taacgcgatc caggcgatac tcagtatggg
    12541 atgacggcca acaaattgca taatttcttg catggggggt aacaactccc aacgtagtga
    12601 ttaaaaaaac caggaaagga gtatacctgc gcggtgcggc aaatacagcc agcgcgttaa
    12661 ctggaatgca attttgcggg gcacgacgaa aaaaaagcgc aaatctggca tcggcttgcc
    12721 gcgagcggtc gtatattttg atcttcagag gctattttat cgattcagct gtagtaaaat
    12781 tacgcaaatt ttgactcttg agtatgaggt tgtcgcaatg tcggtttcta aaaaacctat
    12841 ggtactggtg attctggatg gctatggcta tcgtgaagaa cagcaggata acgccatttt
    12901 tagtgctaaa accccggtaa tggatgcact gtgggccaat cgtccgcata ccctgatcga
    12961 cgcttccggt ctggaagtcg gtctgcctga ccgtcagatg ggtaactccg aagtaggcca
    13021 cgttaacctg ggtgccggcc gcatcgtgta tcaggacctg actcgtctgg acgttgaaat
    13081 caaagatcgc gctttctttg ctaatccagt gctggctggc gcggtacata aagcgaaaaa
    13141 cgcaggtaaa gcagtacaca ttatgggtct gctctccgca ggcggcgtac acagccacga
    13201 agatcacatc atggcgatgg tagaactggc agctgaacgc ggcgcagaaa aaatctacct
    13261 gcacgcattc cttgacggtc gcgacacccc gccgcgcagt gctgaatcct cgctgaaaaa
    13321 atttgaagag aaatttgctg cgctgggtaa aggccgcgta gcgtccatca ttggtcgtta
    13381 ctacgcgatg gaccgcgata accgttggga tcgcgttgaa aaagcctatg acctgctgac
    13441 tctggcgcag ggtgagttcc aggccgatac tgccgttgct ggtttgcagg cagcttatgc
    13501 tcgcgacgaa aacgacgagt tcgtgaaagc gaccgttatt cgtgcagaag gtcagccaga
    13561 tgcggcaatg gaagacggcg atgcgctgat tttcatgaac ttccgtgctg accgcgcgcg
    13621 tgaaatcact cgtgcattcg tgaacgctga tttcgatggc ttcgcgcgta agaaagtggt
    13681 taacgtcgat ttcgtgatgc tgaccgaata cgccgctgac atcaaaactg cggttgctta
    13741 cccacccgct tccctggtta acaccttcgg cgagtggatg gcgaaaaacg acaaaactca
    13801 gttgcgtatt tccgaaaccg aaaaatatgc ccacgttact ttcttcttca acggtggcgt
    13861 agaagagtcg ttcaaaggcg aagatcgcat tctgatcaac tcaccgaaag tggctaccta
    13921 cgatctgcaa ccggaaatga gctccgcaga gctgaccgaa aaactggttg cggccatcaa
    13981 gagcggcaaa tacgacacca ttatctgtaa ctatccgaac ggcgacatgg taggtcacac
    14041 cggggtgatg gaagcggcgg ttaaagcggt tgaagcactg gatcactgcg tggaagaagt
    14101 cgcgaaagcg gttgagtccg tgggtggaca actgctgatc accgctgacc acggtaacgc
    14161 tgaacagatg cgcgatccgg caacgggtca ggcacacacg gcacacacca acctgccagt
    14221 tccgctgatt tacgttggtg ataagaacgt gaaagcggtt gcaggcggca aactttctga
    14281 catcgcgccg accatgttgt cgctgatggg tatggaaatc ccgcaagaga tgactggtaa
    14341 gccgctgttc atcgtggaat aatccctccc catgagggga aaggcgatta ataccatgac
    14401 acgggccgtg aaaccgcgca ggtttgcaat caggcccatc atctacgcca gcgttcttag
    14461 cgctggcgta ttgttgtgcg ccttttccgc ccacgcggat gagcgtgacc aactcaaatc
    14521 tattcaggcc gatatcgccg caaaagagca cgcggtacgc caaaagcaac aacaacgcgc
    14581 aagcctgctc gcacaattga aaaagcagga agaagcgatc tctgaagcca cccgtaagct
    14641 gcgcgaaacg caaaacacgc tcaatcaact caataaacag attgatgaga tgaacgcgtc
    14701 gattgccaaa ctggagcagc aaaaagccgc ccaggagcgc agcctcgccg cacaactgga
    14761 tgccgcattc cgtcagggcg agcataccgg tattcagcta attctcagcg gtgaagaaag
    14821 ccagcgtgga cagcgtttac aggcttattt cggctatctc aaccaggcgc gacaagaaac
    14881 cattgcccag ttgaagcaaa cgcgtgaaga agtcaccatg cagcgtgctg aactggaaga
    14941 gaaacaaagc gagcaacaaa cgctgttata tgaacagcgc gcccaacagg cgaaactgac
    15001 tcaggttctg aacgagcgta aaaagacgct ggcggggctg gaatcttcca tccagcaagg
    15061 tcagcagcag ctaagtgaac tgcgtgccaa cgaatcccgt ctgcgtaaca gcattgcccg
    15121 tgcggaagcc gcggcgaaag cgcgtgctga acgagaagcg cgtgaggccc aggcggttcg
    15181 cgagcgccag aaagaagcga cgcgcaaagg caccacctac aaaccgaccg aaagcgaaaa
    15241 atcgctgatg tcccgaactg gtggcctggg tgcgccgcgt ggtcaggcat tctggccggt
    15301 tcgcgggccg acgctgcatc gctatggcga gcagctacag ggtgaattac gctggaaagg
    15361 gatggttatc ggtgcctctg aaggtactga agttaaagca attgccgacg gtcgagtgat
    15421 tctggctgac tggctgcaag gttacggtct ggtggtggtg gttgagcacg gtaaaggcga
    15481 catgagtctt tacggctata atcagagcgc actggtgagc gttggttcgc aggttcgcgc
    15541 gggccagcca attgcactgg tgggcagcag tggcggtcag ggtcggcctt cactctattt
    15601 cgaaattcgc cgccagggtc aggcggtcaa tccacagccg tggttgggaa gataagtttt
    15661 gtttccattt cgtcgtaacg ttcttgcatt tgccgctctg ttggcgctct cctcccccgt
    15721 acttgctggc aaacttgcca tcgtcattga tgattttggg tatcgcccgc acaacgaaaa
    15781 ccaagtgctg gcgatgcctt ccgctatctc cgtcgctgta ttacccgatt caccgcacgc
    15841 cagagagatg gcgaccaaag cgcataacag cggtcacgaa gtgttgattc atctcccgat
    15901 ggcaccgttg agtaaacagc cgctggagaa aaatacgcta cgcccggaga tgagcagcga
    15961 cgaaattgag cgcattattc gtagtgcggt caataacgtg ccctatgccg tggggatcaa
    16021 caaccacatg ggcagcaaga tgacctctaa cctgtttggt atgcagaaag tgatgcaggc
    16081 gctggagcgt tacaatcttt acttcctcga cagcgtaacc atcggtaata cccaggcgat
    16141 gcgcgccgcg caaggtactg gcgtgaaggt gatcaaacgg aaggtgttcc tcgacgattc
    16201 gcagaacgaa gcggacatcc gtgtgcaatt taatcgcgca attgatctgg cgcgtcgcaa
    16261 cggttcaacc attgccattg ggcatcctca cccttcaacg gtacgcgtgc tacaacagat
    16321 ggtttataac ctgccgccag acattacgct ggtgaaagcc agcagcttgc tgaatgaacc
    16381 gcaggttgat acttctacac cgccgaaaaa cgctgtgcct gatgcaccgc gtaatccgtt
    16441 ccgtggcgtg aagctgtgca aaccgaagaa accgatagaa cctgtttatg ctaatcgctt
    16501 ctttgaagta ttaagcgaaa gcatcagcca gagcacgctg atcgtttact tccagcatca
    16561 gtggcaaggc tggggcaaac agcacgaagc ggcgaagttt aacgctagcg caaattaaag
    16621 gcgctacgcg taatgcgatg gtgcgaccgc gttttgtcgc gccattgcca taagcggaaa
    16681 gaccacagta acgcctggta accgaccttc acgctgcgca cattggtaat caggcgctta
    16741 tacataccgg aagtaaatat ctcggcaatc atccgttggc gggtaagaat atccggctct
    16801 ttgcgcaccg catggcaaac acgcaatgct tcgtaagtta tttgctgatg aaattccgga
    16861 taaatcgtaa ttttgtcggc ataatttcga tttaatttct ccagcaggcg ggtaatctta
    16921 atatagtgac gttgataatt aaggttttta ttcccttgtc tatgcaaccg actcactgac
    16981 gtattatgca gataatattt atataatgat tgctcggtat atcgcgcacg cagcgcgtta
    17041 aacatgaatt ctgttgtcca gacaatatcc tgatgatgta atccggcaat aaatttaatg
    17101 ttatttttaa caataacatc acggcgataa acccccatcc agacaacgtg agtccaacgg
    17161 cgcgaagaaa gccccatccg cagccagtcc gggccggtta atacaccggt tgagcgaagg
    17221 cgatcgctgg ggatggattg ccaggtttct cccgtttcac gaaaagacca gtcagcgttg
    17281 cactgcgcca cgtcgaggtc gtcctctaac gccatggtca tcagcgtttc gtacatggtg
    17341 ggatagacct catcgtcagc atcgacaaaa gcgacatatt tgcccgtcgc cacctcgatc
    17401 ccacgattac gcgctaccga tgcgccagca ttcgcctgat gcaacaaacg aacgtgcgga
    17461 tagttttctg cgtaatgctt tgctatttca acagaattat ccgttgaacc atcgttaata
    17521 ataatgattt ccagagcagt ccaggtttgc gtaattaaag attccataca agtgcggaaa
    17581 tcatcgcccg cattatataa cggaataata acactaagtt tattggtgct gttcatcata
    17641 aattaccgac gacctctgaa gttatcctga cgttttacag gctttgtatt aagaaactat
    17701 taagcctgtg agatattatt tttacaggtg agagagtcac ttcagggtag agagataatg
    17761 agaacagcta tataaaagat cagcgcaatt acgaactctt tattacactg acaggatttt
    17821 acataattaa cagattatcc tcagataaaa gggctggaat accagccctt gttcgtgtta
    17881 atcccagctc agaataactt tcccggactg gcccgaacgc atagcgtcaa agcccttctg
    17941 gaaatcatcg atagagaaac gatgggtaat gatcggcgag agatcgaggc cagactgaat
    18001 cagcgccgcc atcttgtacc aagtttcaaa catctcacga ccgtaaatgc ctttaatgaa
    18061 caagccttta aagatcactt tggtccagtc gatagacata tcagacggtg gaatacccag
    18121 catcgcaata cgaccgccgt ggttcatggt atcgagcatg gtacgaaacg ctggcggcgc
    18181 gccggacatt tccagaccga catcaaagcc ttcggtcatg cccagttcag ccatcacatc
    18241 attaaggttt tctttcgcaa cgttaaccgc acgggtgata cccattttgc gcgccagctc
    18301 aaggcggtat tcgttaacat cggtgatcac cacattgcgt gcaccaacgt gtttcgccac
    18361 cgccgcagcc ataataccta tcggacctgc accagaaacc agcacatctt cgcccaccag
    18421 atcgaacgac agcgcggtat gcacggcgtt accgaaggga tcaaaaatgg aagccaggtc
    18481 gtcggagata ttgtcgggga ttttgaaagc gttgaacgcc ggaatcacca gatattcggc
    18541 gaagcagccc ggacggttaa caccgacacc aatggtgttg cggcacagat gggtacgacc
    18601 gccacgacag ttgcgacagt gaccgcaggt gatatggcct tcgccagaaa cgcgatcgcc
    18661 gattttgaag cctttcactt cctgaccaat acctaccact tcaccgacat attcatggcc
    18721 cacgaccatc ggcaccggga tggttttttg cgaccactca tcccagttat agatgtgaac
    18781 gtcagtcccg cagatggctg ttttacggat tttaatcagc agatcgttat gcccgagttc
    18841 cggtacaggg acgtcggtca tccagatgcc ctcttccgct ttcagtttgg ataacgcttt
    18901 catctcacat cctcaggcga taacgcccag ttgtttacca atacgcgtga atgcttctac
    18961 tgcacgcgta atttgctcag gggtatgcgc cgcagacatc tgggtacgaa tacgcgcctg
    19021 acctttcgga acgaccggat agaagaaacc ggtgacgtaa atgccctctt tttgcagctc
    19081 tcgggcaaat ttctgcgcca ctaccgcatc accaagcatg accggaataa tggcgtgatc
    19141 ggctcccgcc agggtaaagc ccgccgccga catttgctca cggaactgac gcgcgttcgc
    19201 ccacagacgg tcacgcaact cgctacccgc ttcaaccatc tccagcactt tgatggaagc
    19261 ggcgacaatc gctggtgcca gcgagttgga gaacaggtac ggacgagaac gctggcgcag
    19321 ccactcaacc acttctttgc gcgccgcggt ataaccacca gaagccccgc ccagcgcttt
    19381 accgagcgtg ccggtgatga tgtcgacacg ccccatcaca tcgcagtatt catgggaacc
    19441 acgaccattt tcaccgacaa aaccgaccgc gtgggagtcg tctaccatca ccagggcatc
    19501 gtatttatct gccagatcgc aaacgccctt cagattggca atcacgccgt ccatcgagaa
    19561 cacgccatcg gtggcaatca gcacatgacg cgcaccggct tcacgcgctt ctttcagacg
    19621 agcttcaagc tcctgcatat cgttgttggc atagcgatag catttagctt tacacagacg
    19681 cacaccatca ataatagacg cgtggttcag tgcgtcggag ataatggcgt cttccgcgcc
    19741 cagcagcgtt tcaaacaggc caccgttagc atcaaagcag gaagagtaga gaatcgcatc
    19801 ttccatcccc aggaatgccg ccagtttttg ttcaagctct ttatggctgt cctgagtgcc
    19861 gcaaataaaa cgcaccgaag ccatgccgaa accgtgagaa tccattcccg cctttgctgc
    19921 cgcaatcaga tccggatgat tcgccagccc gagatagttg ttggcacaaa agttaatgac
    19981 gtggcttcca tcagccacgg tgatatctgc ttgctgcgca gacgtaataa tgcgctcttc
    20041 tttaaacaac ccttccgccc gtgcggtttc cagatcgttg gttaactgct gataaaattc
    20101 tccacgcatt gcgattctcc agacagggca aattccagca catattaccc aaacttatag
    20161 gtcgcgacga gatgatgcgt taacacttct gcaaaattca ggataaataa cgtgtatctt
    20221 gatgtctctc aacacctaca gatagtagaa aaactgtaca gttttggctg agcataatga
    20281 tgagtgattt tactgcattg atataaaaag aaaaaatgat gccaaagcat catttttcct
    20341 atctcagttg gcatcaaaac gctacccact aatggaggga aacctatttt cttgaaagga
    20401 aaattctcag cgcctgcatt tttgccccta aagtgttact cctgaagcag cgaaacaaat
    20461 cgaaccattt tcctctcccc agatagttta ggttgaagag atcggaacgt ttgtaatagc
    20521 acatgacaaa aatgccctgg tcatcgtcca caatgttatt gtttagtgtt attttttgac
    20581 tttccagaac taatttatag aattctttcc atttatgctg tgaaccaaca attgcgcccc
    20641 caataatata tacatgattg ccaatcatga aatcaaaagc ctgttgttga gatgtaacag
    20701 taagcccttt ttttatagtg aaaagatgca tcttattttc atcaaatggg aaatcccaaa
    20761 ttttcaatcc tcgagttaca tttggcttac ggcaatagcc aaaatctatc cacgcaacaa
    20821 gaggcgtttt cactaaaccc atgttgatag ctttatttac aaaataggcc ttaaggttac
    20881 ataccaaaac atactctggt gaccagtact ctgggttttt taattgtcga ggctcaagtc
    20941 tatttgtaaa cgactcatct ttttgaattt tttcgatacg acttctgata tatctaaatt
    21001 tcttttttat atcgataacg ataaccgttg ttggttttcc gttgcgaatc gcttcaatcc
    21061 ttggtttcaa atcgggagaa gtgaaaataa tcatctcatt ttcaagtgcg gccagacgct
    21121 caaagtaact aaaataaaca tcaactgaac gagctagttt ttcacggaac cctttattag
    21181 cagtccaatc ccctctacca atgtcaaaat atgcagtgat aattgtggtg gatgatttca
    21241 ttactaataa tgaccattaa tataaaaaaa gaattatatc ataaatatta acagaatgta
    21301 tactgtatat tatccattca aatgattccg ttatttatca tacatcggaa tattgatact
    21361 aaagcactat tcacatgcaa aaccaacatc cgccatgaag gactagctaa aacccaaact
    21421 agtttgttgc aattagcatc cttgcacctc tatgtaaagg gctgaaggga ttcggatgtg
    21481 atggtatgat tacagacatt cgtgtctgag attgtctctg actccataat tcgaaggtta
    21541 cagttatgat catcgttacc ggcggcgcgg gctttatcgg cagcaacatc gttaaggccc
    21601 tgaatgataa aggcatcacc gatattctgg tggtggacaa cctgaaagac ggcaccaagt
    21661 ttgtgaacct ggtggatctg gatatcgcgg actatatgga taaggaagac ttcctgatcc
    21721 agattatggc tggcgaagag ttcggtgatg tcgaagcgat tttccacgaa ggcgcatgct
    21781 cttccaccac cgagtgggat ggcaagtata tgatggataa caactatcaa tactccaaag
    21841 agctgctgca ctactgtctg gagcgcgaaa tcccgttcct gtatgcctct tccgcagcca
    21901 cctacggcgg acgcacctcc gactttattg aatcccgcga gtacgaaaaa ccgttgaatg
    21961 tctacggtta ctcaaaattc ctgtttgatg aatatgttcg tcaaatcctg ccagaagcga
    22021 actcgcagat tgttggcttc cgctatttca acgtttatgg accgcgtgaa ggccataaag
    22081 gcagcatggc gagcgtcgct ttccatctca acacccagct taataacggt gaatcaccga
    22141 agctgttcga aggtagcgag aacttcaaac gtgacttcgt ctatgtgggc gacgtggcag
    22201 atgtaaacct gtggttcctg gaaaatggcg tttccggtat cttcaacctc ggcaccggtc
    22261 gtgcagaatc cttccaggca gtagcagatg ctacgctggc ttatcacaag aaaggccaga
    22321 tcgaatacat tccgttcccg gataagctga aaggccgcta ccaggcgttc actcaggcag
    22381 atctgacaaa tctgcgcgcg gcgggttacg acaaaccgtt caaaaccgtt gctgaaggtg
    22441 taacggaata catggcctgg ctgaatcgcg acgcataaga gctctgcatg aaaatactgg
    22501 tgatcggccc gtcttgggtt ggcgacatga tgatgtcgca aagtctctat cgcacgctcc
    22561 aggcgcgcta tccccaggcg ataatcgatg tgatggcacc ggcatggtgc cgtccattat
    22621 tatcgcggat gccggaagtt aacgaagcta tccctatgcc tctcggtcac ggagcgctgg
    22681 aaatcggcga acgccgcaaa ctgggtcata gcctgcgtga aaagcgctac gaccgcgcct
    22741 acgtcttacc aaactccttc aaatctgcat tagtgccttt cttcgcgggt attcctcatc
    22801 gcaccggctg gcgcggcgag atgcgctacg gtttactcaa cgatgtacgc gtgctcgata
    22861 aagaagcctg gccgctaatg gtggaacgct atgtcgcgct ggcctatgac aaaggcatta
    22921 tgcgtaccgc acaagatctg ccgcagccat tgttatggcc gcagttgcag gtgagcgaag
    22981 gtgaaaaatc atatacctgt aatcaatttt cgctttcatc agaacgtccg atgattggct
    23041 tttgcccggg tgcggagttt ggtccggcaa aacgctggcc acactaccac tatgcggaac
    23101 tggcaaagca gctgattgat gaaggttatc aggtggttct gtttggctct gcgaaagatc
    23161 atgaagcggg caatgagatt cttgccgctt tgaataccga gcagcaggca tggtgtcgga
    23221 acctggcggg ggaaacacag cttgatcaag cggttatcct gattgcagcc tgtaaagcca
    23281 ttgtcactaa cgattctggc ctgatgcacg ttgcggcggc gctcaatcgt ccgctggttg
    23341 ccctgtatgg tccaagtagc ccggacttca caccgccgct atcccataaa gcgcgcgtga
    23401 tccgtctgat taccggctat cacaaagtgc gtaaaggtga cgctgcggag ggttatcacc
    23461 agagcttgat cgacattact ccccagcgcg tactggaaga actcaacgcg ctattgttac
    23521 aagaggaagc ctgacggatg cgggttttga tcgttaaaac atcgtcgatg ggcgatgttc
    23581 tccatacgtt gcccgcactc actgatgccc agcaggcaat cccagggatt aagtttgact
    23641 gggtggtgga agaagggttc gcacagattc cttcctggca cgctgccgtt gagcgagtta
    23701 ttcctgtggc aatacgtcgc tggcgtaaag cctggttctc ggcccccata aaagctgaac
    23761 gcaaagcgtt tcgtgaagcg ctacaagcag agaactatga cgcagttatc gacgctcagg
    23821 ggctggtaaa aagcgcggca ctggtgacac gtctggcgca tggcgtaaag catggattgg
    23881 actggcaaac cgctcgcgaa cctttagcca gcctgtttta caattgtaag catcatattg
    23941 caaaacagca gcacgccgta gaacgcaccc gcgaactgtt tgccaaaagt ttggactata
    24001 gcaaaccgca aacccagggc gattatgcta tcgcacagca ttttctgacg aacctgccta
    24061 cagatgctgg cgaatatgcc gtatttcttc atgcgacgac ccgtgatgat aaacactggc
    24121 cggaagaaca ctggcgagaa ttgattggtt tactggctga ttcaggaata cggattaaac
    24181 ttccgtgggg cgcgccgcat gaggaagaac gggcgaaacg actggcggaa ggatttgctt
    24241 atgttgaagt attgccgaag atgagtctgg aaggcgttgc ccgcgtgctg gccggggcta
    24301 aatttgtagt gtcggtggat acggggttaa gccatttaac ggcggcactg gatagaccca
    24361 atatcacggt ttatggacca accgatccgg gattaattgg tgggtatggg aagaatcaag
    24421 tagagtgtcg ttctacaagt atgtctcttg cagatttgcc agctcaaacc gtttttcaaa
    24481 atttaaatct tgaaataata accaataagt tgacatcgga gataagatga cctcaacatt
    24541 atttttctct ctcgagaaaa aaaactggat agcgtactgg aacagagctc tcgtattctt
    24601 attcattacc acctattttt tgggtgggat tacaaggtat aaacatctta ttgttattct
    24661 tatgacaata acgacaatcg tctatctctg caaacggcca aaacactatc tctcactatt
    24721 taaaacattt ctttttggta gtgttgccat attaactatt gctgcattgc tgtcacttct
    24781 tcaatcccct gatgcaggtg ctagcatgaa ggaaattttc aaagctatta ttgagaatac
    24841 tttactatgc acaatagcaa taccggtcat attgagagac gagaaaagag aagatgtcga
    24901 aaaaatcgtt ttcttctcat ttattagtgc gttgggctta cgctgttttt ctgaattgat
    24961 tacctattat aaggactatc aacaagggat aatgccattc gcagattata gacaccgtag
    25021 catttctgac tcgatggtct ttttattccc tgcattgtta aatctctggc ttatcaaatc
    25081 agcaaaatac cgcatttctt ttgtggttct aagcgttatt tttatttttc tgatattagg
    25141 aactttatcc agaggggcct ggctttccgt gttagtcatt ggattaatat ggattctgat
    25201 gtttaaacaa tggaagttac tattagtagg agtaatggtt tccatcattg cattgtcggt
    25261 tattttcaca cataaggaga tgaccgcaaa gctaacgtat aaacttcaac aaactaatag
    25321 ttcttatcgc tatgcaaatg gtactcaagg cagcgcactc gatctaatat tagaaaatcc
    25381 tgttattggt tatggttacg gtaacgttgc atataaagat gtctataata aacgtgtcat
    25441 tgattatcca gaatggacct ttagacaatc aatagggcca cataattttg cgctattcat
    25501 ctggtttggc actggtttat tagggctggt aagtcttatg atgctatact gtgcaatatt
    25561 gaaagagtgt ataaaaaatg gcgtcaagaa taaatatcgc tcaccatata atgcatatta
    25621 tataatctta ctatctttta taggttattt tgttatccgt ggaaacgtag aacaaattga
    25681 accaaattta ttaggcgttt acgccggctt attattagcg atgaaaaaca agtaaaaaat
    25741 aaaaaaggct gcataatgcg gcctttttat ttattcaaac caattatgaa taacctcttc
    25801 gaatctttga gttacgccat cccaactgta gtgatcaaac acaaagtcct gaccttgctt
    25861 tgcaactgct gttaattcag gattcgctaa cgtttttaaa atatcgctgc ttatggagtc
    25921 tgcagtcata ggttctttta aatggaatcc cgtagtattt tctttgacaa attccgtcat
    25981 ccccccacgc gtactcacta aaacaggctt accagcaccc atagcttcaa ttgccaccat
    26041 gcaaaatggt tcctggaact gggaaggaat gacgactaaa tctgccagtg ggtaataaca
    26101 atgcatcttt tcaggcggta tgcttcctaa cataatgcaa cgatccttca aacgttttgc
    26161 aatttccctg acattgcgtt gatatgcacc tttgtcactt ttactcattt ctgtataatc
    26221 accaatcaca actaatttta aatttttgtg agcagcggcc aatttttcga atgattgcat
    26281 caacagcaat atgcctttgt cagggacgat acgcccagca taaaaaataa ttttctcttc
    26341 aggggagata ttaagttcgc ttctagtgat cggctgaaaa ttagattgat aggtttctaa
    26401 atcaattcca tttggtacaa tctcaatatc tgcatttgct aaataagatt ggtaatactt
    26461 tttcaagtat aaactaggga ctatcatttt aacattttgc tcaagcaatt ttggttcaaa
    26521 tgcattatgc atatgtataa ccactcttgc ctgaggagct cgcttacgaa tttgagtgta
    26581 tagcttcatg ctattatgaa ctacaataac actttctttc gttataggaa aatcatgtgc
    26641 aatattcaat attctctgtg aatatggaag aggatcaagt cttgtccatt tttgaaataa
    26701 ccgcttatat atgcgactga atccaaccct atgaatacta caattatcat taacaaaggt
    26761 gtaatttgag tatcctggat ttttgataca aacaatacga tttggaaaat tggtacgctg
    26821 agcaacctga tacatccacg tttcaacagc agccgctccg cgaggaggaa ttgaaaaaat
    26881 aggagtaacc gtaaatatta ttttatcaac catctcgtat attaaccttt cataacatta
    26941 tatttaattg ctgtaaaaac accatcgaga aattttttct ggtaaagcaa gtgtttatat
    27001 ttttctttgt gctcatgttt tttaacagct tttttataag gtatatttct ccatggtgat
    27061 atattataaa tattacgaaa ataatctgca gaggcgtagt tcgcccaatc atgccatggc
    27121 ttagttatac cagtataatg tatgaagaca gtgtcatcat taataaaccg ggtgtaatat
    27181 tcactatttt tttcttcaaa ttctgacttt atagtataaa tacaattata ttttctcggt
    27241 aatattttag catgatgtag aaacattaag tttaaaatat cttgatccgg gtactttaat
    27301 ttttttataa ttgactcatc tgataataga tcaaagaatt tttccgttag tctttgtttt
    27361 aaccattccc gtaaattaat atacattaca ccagagttaa aatagctacc gttaaaatcc
    27421 tcattacaca aacgctcagc gcttttactt tgcatagcat ctacatcaat taccactgcc
    27481 ccatattcat ctttaaactc taatgctatt aactcgttca atgaaccctt acagacgata
    27541 tcagcatcaa gatataataa tgaatccaat cgcgcagaaa aataatcaaa tgaaagcaaa
    27601 cgataataaa tagatactgg ccagatattt gattgaggta gcgccttcaa tggctcagaa
    27661 tcaatatgat ataatttaat tactgtttga tacttcgttg ctaattgtga taataattca
    27721 atatactctt tgtctaagta atcagcgatt atgtgaaagg taaatttgtt ttgtttattc
    27781 ttttcaagaa ttgaagcaat tgaaatcgca gccccaacct ggtagttctc atcaataccc
    27841 catgagacat taaacgtttc ccgttcatca agtttaacgg ggcgcttgtc cagttcaatt
    27901 atatctttaa attgagtaag atgtttaaaa tccaatttaa ttacccttca tcataatcat
    27961 tatttgctct tcaatcctag tttgactttt acatccctga ttactttttt aattttcttt
    28021 ttgaatataa gatatgtata gccaaagtct ttcaattcat tttttatatt atagtgccgt
    28081 tcaagatcaa tgcgatcttt tgcttttaaa acagcggttg tttttttacc tgaaaggtca
    28141 attagtctta aacctttttc ggatactata aaatttcctt tatgaggatc gccagacacc
    28201 attccatgct gatgcaactc ttttatcgat tctgataatt gatccttcag atcttcagaa
    28261 atttccaaat actcatttaa cccgacacct tcaatatact caatgagcat aatataataa
    28321 tgagcgaaat ttaatgtttt acgttcagct agcaaaaaat agtcattaat tgattggatg
    28381 ccttcaccac gtacccgatc ggtttgataa attaaatttt cataataatc tttttttatg
    28441 caagatttta agaaacgttc ggtcatttta tgctttggcg cataaacttt aagtacaaga
    28501 ggtccacgag cagtgtcaat aagaatgact ttagtatcat caatagaacg aaaaactttt
    28561 aatgttttaa ccctgcaagt taaaaagtca tttagaactt gctcataaaa gggatcatta
    28621 tcttttatga aaactttcaa cccatttatc gttttattat atatcataat gaattatttt
    28681 aaccttaaat ctttagaagc atttttcttt ataatacttt aaataattaa aaatgcctgc
    28741 catataatgc ttttgtttaa atttatgttt tgcacaatac cgatactgat tgctatttac
    28801 aggtttaagt aaatcttctt tactccacgg agaagctgct ttggcgatca aaaaactacg
    28861 tgaaaccgga taatcagccc actcatgcca gggttttgta ggtcccacat agtgtataaa
    28921 cacggtgtca tcattgactg gattatcaac tttgtctttt aattcatagt taatactata
    28981 tcgggtatta tatttctccg aaataaactt cactttacca ttcaataata cattcagtac
    29041 atcttgatca aggtgggtga ttttacttac ccaatctggg tcacgcaaca tttcaatagc
    29101 ttttgacgaa atgttattta gattccactc atcaatattt atcagtaaaa aaccagcatt
    29161 gaaatatcca gaagctaact gtggtgtagt taataccgag gctcgatttt gccaccattc
    29221 aatatctctt tcagctacaa ctgcggcaat ttcattagta gaaaattgat aatctaagag
    29281 ttctttaata ctacccttgc aagcaatatc tgcatcaaga tatagtattt tttcatgttt
    29341 atgatagaaa taatctgcaa ttataaatcg aaaatatgtt gcgtaagtcc agtttttcgt
    29401 gcttggtaat gacttcagct tatcacaatt gataacataa atattaattc ttgaattata
    29461 ttgttttgct aaatcagaaa aatataattt gtctttatca ctgatataat ctgtgaaaac
    29521 atgaaattca caagagattt ctctattgtt taatagaata gatgcgatgg ctacaccaca
    29581 tccaaaaaga aaatttctgt caataccaaa tgctatatca agagtatttt tagatcttat
    29641 attttgataa tgaaaattat actcaaaaag gatgatgtca ctatcattga gttgagacat
    29701 atttattctg tatccaaaat tttggcaaaa ataagttacg ggttacaacg attttcgaat
    29761 cgttctttcc ctgatttttg tggcttttgc ttctgcttgc gacagcagtc cttgttcctg
    29821 cttgagaatg tctttaagcg gggcggcaaa atacaccttc ataaagcgcc agatatcccg
    29881 ctgagtcagg ccaatattca ttgaagaaaa ataaagccct ataagatctt tatcgcgcca
    29941 acgacctgga acgcgcgtgc gaagctgcgc ccggtgtagg tcaattaccg aaatttttaa
    30001 ctcctcttcc ttaccggaaa aaggcaagtg cagcaggaaa tgacagatat aacagtcacg
    30061 atggttaatg cccgcagcat gcatatcgcg caccatcgtc gcgacacgct taataagcat
    30121 acgctttacg cgaacatctg gtgggttggt cgcccagtca gcacagtaat cttccagact
    30181 gatggttggt gtcagatctt cggtaataat aaacgaggtt ctggtcagcg gattcatgcc
    30241 tttttcgccg aatgccaccc catacatagt atcaacgccg acatcccgca gtcgatgaat
    30301 cgcattccat tcgcggtctg cgcctaatac tggcatccgc aatgagagta aatttttgat
    30361 tatctctttc agggtcgtgc cgcgatgcca tttgagaaaa tagctttttc ccgccatttc
    30421 aaagcgcaga gtacggcgag tttccagttc acgaaatacc tcaccctgca aggttttaac
    30481 ttcctcaaaa ggatctttgc cgcgccataa cgtggcaaac ggctctttaa gttcaaccat
    30541 ccagaccacc cgttatgata tccgccgctt tctctggcag actgtataaa tcttgtgtat
    30601 cagcataatg tcgcgcattt tccgcccaag cctggcgcaa tgaagattgc gttaacgctt
    30661 tgcgtaaaat ctcattcaat gtttcctggc ggaatggctc agcaatagcc tcgccgcaat
    30721 tagcgtcgac aatataatgc gcatagccac aaacggcagt tgttagtacc ggtaatcctg
    30781 cagtaatcgc ttccagcagc acaattcccg ccgcttcctg gtaggcagga tgcagtaata
    30841 aatccgccgc cgccattaat tccgagacat cgttgcgccc cgagaagaag tgaacattac
    30901 tgcgcacgcc gcgtttttct gccagtgcct caaattttcg cggtttatcc tgcccaacaa
    30961 catatagcaa tgtgttgtgg cgcagcgaat ccggtaacga agcaagtgct tcaatggaac
    31021 gatcgacacc tttacgcgtg aagtctgaac cgacctgcaa caataaatat tgttgttcgg
    31081 ttattccatt cttcttacgg aagatttcac ggctattggc tggctgctgg ctatatttac
    31141 gatcaggata aatccccggt ggcagaatat gaaaacgctc cgcttcagtc tgataatgtt
    31201 tctggaaatc ggcgatttgc ttatctgtca gcatcagcag ctgtgtcggc ttgccctgtt
    31261 cgaaggttgc ccgctcaaag gcggcataat ggcgataacg tgacgtcagg cgatagaaaa
    31321 agcctttttc ctgcgctact ttctcggcat aacaaacatc agcggcataa taaacgtcca
    31381 gccccggcat tttgttgaat ccaacgactt tatcgacggg atgttcgcgt aaatgttttt
    31441 gcacccaggc aaaatactcc gcattgcgcc cgtgattggt atgcgattta accggcactt
    31501 tgatcagttc aaatacatca gggcattcgc cttcccacga ctgggtataa acccgaacat
    31561 gatgacctcg ggcggcgact gtctgagcaa tacgcataaa atcgcgctgc aaaccgccaa
    31621 agggaaaata tttatataaa caaaaagcaa cgatcataac gacgtacctg tcgtggagga
    31681 gggcagtaat ttatcgacag cagcaatgac atctgccgcc ggaataaccg aaagatacat
    31741 ctcatttcgg tcacgctgat cgcgcgttgg catttcccgg taatctcccg cccagaattg
    31801 aatcatgtta tttgaccagg gacgccagaa aatatgatct gttgcgccaa acagcgatat
    31861 cagcggcgta ttaactgcag cggcaatatg cgccggtgcg gaatcaacgc caataaacag
    31921 ctgcgcatga tcgattaacg caccaagttc cgggaaggtc acctttccag ccagcgccgt
    31981 tactggtggc gtctggcatc cctgcgcaat ttcattgacg caggccagat cgtctttatc
    32041 tgggccggac gtcagaacga cttcataacc acgagcatgt aaggcatcaa tcacagcgga
    32101 aaacttggcg ttgtcccagc atttgaagat ttgccgcgcc gtaggttgga taaccacata
    32161 attttgtcca acaccagcgt gatcaagttc gcgacgcata cgtttccagc ttgcaggcgg
    32221 gtaactcatg gttgtctgct tcaccaacga atcaagtccc aatggtgtca gcacggataa
    32281 gttactttcc accacatttc caccctgcaa cggcaccaaa tgggtgaaac ttttacgcca
    32341 aaaagcagac tgccgatgat gataatcctg ggaaattttc acacgagcat ttaataagcg
    32401 aaccagtata gcaaccatcc attgatcggt aagattgacg ataaggtcat acttattggc
    32461 acgtaatacc ttgatgagat gaaaaaagtt ggcaattttt tctgaggctt ttgctttttt
    32521 attttttatg ccgtagagcg cgttaatctc tggattttca gacaggatcg ggatggtgtc
    32581 ctgataaagc agcacatcga tttttgcgtc agggtaattt tttttcagag aactaatgac
    32641 gggagtagtt aataacatat ccccatgaaa acgcatctta atgagcaaaa ttcttcgaaa
    32701 tggcttatcc acaagtaact ctttttgttg agattatccg attaaggtga agcagaaaaa
    32761 gcacgctacc gccccaggct caacagctac ctgaatactg ataccgcact gatattgacg
    32821 cgctagtgta tcacttcttt cgcggcaatc cgacccgaat gatttttaag cacattgttg
    32881 attttcaata aagaaatcaa tcaaataaaa tcattagcaa ccatattctt tcactgttgc
    32941 tctgcgattc cttcctgcac gtacagcaga aatcaatgaa tcgcataaaa atacaaaaaa
    33001 agtatagatg ttcagcaaga attggcgggt aaattgctat ttaagtca
//
LOCUS       DAETPZ010000041        32327 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.041, whole genome shotgun sequence.
ACCESSION   DAETPZ010000041 DAETPZ010000000
VERSION     DAETPZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.041"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="K0W59_002147"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="K0W59_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HBI3023175.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="K0W59_002148"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="K0W59_002148"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBI3023176.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="K0W59_002149"
     CDS             complement(3736..4092)
                     /locus_tag="K0W59_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBI3023177.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="K0W59_002150"
     CDS             complement(4096..4512)
                     /locus_tag="K0W59_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBI3023178.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRSVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="K0W59_002151"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="K0W59_002151"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HBI3023179.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5960
                     /locus_tag="K0W59_002152"
                     /pseudo
     CDS             5738..5960
                     /locus_tag="K0W59_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6464..7657)
                     /gene="tyrB"
                     /locus_tag="K0W59_002153"
     CDS             complement(6464..7657)
                     /gene="tyrB"
                     /locus_tag="K0W59_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HBI3023180.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7910..8989)
                     /gene="alr"
                     /locus_tag="K0W59_002154"
     CDS             complement(7910..8989)
                     /gene="alr"
                     /locus_tag="K0W59_002154"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBI3023181.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9042..10457)
                     /gene="dnaB"
                     /locus_tag="K0W59_002155"
     CDS             complement(9042..10457)
                     /gene="dnaB"
                     /locus_tag="K0W59_002155"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBI3023182.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10540..11523
                     /locus_tag="K0W59_002156"
     CDS             10540..11523
                     /locus_tag="K0W59_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HBI3023183.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11689..11931)
                     /gene="pspG"
                     /locus_tag="K0W59_002157"
     CDS             complement(11689..11931)
                     /gene="pspG"
                     /locus_tag="K0W59_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HBI3023184.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12065..13102)
                     /gene="dusA"
                     /locus_tag="K0W59_002158"
     CDS             complement(12065..13102)
                     /gene="dusA"
                     /locus_tag="K0W59_002158"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HBI3023185.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13465..14449)
                     /locus_tag="K0W59_002159"
                     /pseudo
     CDS             complement(13465..14449)
                     /locus_tag="K0W59_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14767..15282
                     /gene="zur"
                     /locus_tag="K0W59_002160"
     CDS             14767..15282
                     /gene="zur"
                     /locus_tag="K0W59_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HBI3023186.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15324..15533)
                     /locus_tag="K0W59_002161"
     CDS             complement(15324..15533)
                     /locus_tag="K0W59_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBI3023187.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15649..16974)
                     /gene="dinF"
                     /locus_tag="K0W59_002162"
     CDS             complement(15649..16974)
                     /gene="dinF"
                     /locus_tag="K0W59_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HBI3023188.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17047..17655)
                     /gene="lexA"
                     /locus_tag="K0W59_002163"
     CDS             complement(17047..17655)
                     /gene="lexA"
                     /locus_tag="K0W59_002163"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HBI3023189.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17765..18133)
                     /gene="dgkA"
                     /locus_tag="K0W59_002164"
     CDS             complement(17765..18133)
                     /gene="dgkA"
                     /locus_tag="K0W59_002164"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBI3023190.1"
                     /translation="MVNNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18304..20727
                     /gene="plsB"
                     /locus_tag="K0W59_002165"
     CDS             18304..20727
                     /gene="plsB"
                     /locus_tag="K0W59_002165"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HBI3023191.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20882..21754)
                     /gene="ubiA"
                     /locus_tag="K0W59_002166"
     CDS             complement(20882..21754)
                     /gene="ubiA"
                     /locus_tag="K0W59_002166"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HBI3023192.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVASALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21767..22264)
                     /gene="ubiC"
                     /locus_tag="K0W59_002167"
     CDS             complement(21767..22264)
                     /gene="ubiC"
                     /locus_tag="K0W59_002167"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HBI3023193.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLFDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22487..24067)
                     /locus_tag="K0W59_002168"
                     /pseudo
     CDS             complement(22487..24067)
                     /locus_tag="K0W59_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24296..25216)
                     /gene="malM"
                     /locus_tag="K0W59_002169"
     CDS             complement(24296..25216)
                     /gene="malM"
                     /locus_tag="K0W59_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HBI3023194.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKIQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLIIDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTMEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25368..26708)
                     /gene="lamB"
                     /locus_tag="K0W59_002170"
     CDS             complement(25368..26708)
                     /gene="lamB"
                     /locus_tag="K0W59_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HBI3023195.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26780..27895)
                     /gene="malK"
                     /locus_tag="K0W59_002171"
     CDS             complement(26780..27895)
                     /gene="malK"
                     /locus_tag="K0W59_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HBI3023196.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28260..29450
                     /gene="malE"
                     /locus_tag="K0W59_002172"
     CDS             28260..29450
                     /gene="malE"
                     /locus_tag="K0W59_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HBI3023197.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29604..31147
                     /gene="malF"
                     /locus_tag="K0W59_002173"
                     /pseudo
     CDS             29604..31147
                     /gene="malF"
                     /locus_tag="K0W59_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
     gene            31162..32052
                     /gene="malG"
                     /locus_tag="K0W59_002174"
     CDS             31162..32052
                     /gene="malG"
                     /locus_tag="K0W59_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HBI3023198.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
BASE COUNT         8229 a         8289 c         7801 g         8008 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtgaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgcc tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acgctgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctcttatg ccaaagattt ctttttatac atagaaacac agttaaaaat
     5881 tgcaaaagat ttttgtagac ctggagaaga agtatcaagt tctattgcaa gtaaagtttt
     5941 tcacgcattt attgatttag ttaacaaaat caggggtaag aaagacttta tgtatatttg
     6001 cacgctttgc tgttttgccg aggaggttaa aggtgattat tctcattaca ggaccttttt
     6061 atttgatatt ggtaatcaat ataaggttaa acttatacaa agcggaaaaa aagagttctc
     6121 tttaacatta gaatttaacg atactataat tgaatctcag aaagtcacag gcaataaggc
     6181 aaagcatatt cttgaggata tagaaatatt ctatcgtaac aaacccgata cttattatta
     6241 aatttaataa tggttagatg tgcagaaatt attactccac acgaaaaaat agggaaagga
     6301 attccagata attgggataa acttaacaat aaaagaactg acaacgtttt attatctctc
     6361 acgtagaaca acggcatcaa aaaaatcatc tacaaagaaa tagtgaaagc gacgacagtt
     6421 taatttcact gcaggctggg taactccagc ctgctttcct gcattacatc accgcagcaa
     6481 acgcctttgc cacacgttgt acatttgccg tatttaaccc ggcgacacac atgcgaccgc
     6541 tggcgatgag atagacacca aattcttcac gtagtcggtc aacctgagcg gcacttaaac
     6601 cggtataact gaacatgccg cgctgattaa gcagataatc gaaattgcgt tctggcatct
     6661 ctgtgcttaa taccttcacc aattcctgac gcattgccag aatgcgagta cgcatctctt
     6721 ctacttccgc cagccagctg gctttcaatg cctcgtcatt cagcactgca gccaccacct
     6781 gcgcaccaaa attcggcggg ctggagtagt tgcggcgaac tgttgctttc aattgcccca
     6841 gtacgcggcc agcggcttcg gcatcttcac acagaacaga aagtccgccg acgcgctcgc
     6901 cgtaaaggga gaaaattttc gagaacgaat tgctcaccag agcgggtaat ccagcgctgg
     6961 caatggcgcg aatagcgtag gcatcctctt ccataccggc actaaatcct tgataggcaa
     7021 tatcgaggaa tgggataagc tcgcgggctt tgagaatttc aatcaccgca tcccactgat
     7081 cattagtgag atcggcaccc gttgggttgt ggcaacatgg atgcagcaac acaatactgc
     7141 gggcaggtaa tgttttcagc gtcgccaaca ggtcattaaa gcgcacgctg ttagtcactt
     7201 cgtcatacca ggggtaagta ctcacttcga atccagcccc ggcgaatatt gctacgtggt
     7261 tttcccaagt aggatcgctg acccagacgc ctgattccgg gaagtagcgt ttcaggaaat
     7321 ccgcgcccac tttcaatgcc cctgagccgc caagggtttg aatggttgct acgcgctgtt
     7381 gttgcagtac cggatggtcc gcaccaaaca gcagcggcgc aatggcatgg cgatagctgt
     7441 taagcccttc catcggtaaa taaagcgaag cgccatgggg ctgcgcattc aggcgcgctt
     7501 ccgcctccgc cacggctttc aattgtggaa taattccgtc ttcgttgtag tacagaccga
     7561 tacttaaatt cactttgtcg ctgcgagggt cttctttaaa acgctccata agcgtaagaa
     7621 tcgggtcgcc agcgtaggcg tcaacttttt gaaacacgcg atggttctcc aggtttacgg
     7681 gcaggtggtt aaaacacaat aaaccggaag aaggcgaaga tcgagtggat gttcaggagc
     7741 gaacggcaat tagcaacaga gtgagactaa tgataaacgt acatccgcca gaggcacgac
     7801 ctttataaga gcatgaaaga acatcaactt attgaatttt taggatttta ttggccggat
     7861 aaggcattca cgccgcatcc ggcacagaca atcaaatatt acagaacgat taatccacgt
     7921 atttcatcgc gacccttgaa gtcaggcgcg taataagttc gtaagcgctt actttcgtca
     7981 tttcagcgat acgttctacg ggcaaacctt cgccccataa aatgaccgga tccccggctt
     8041 tgtcctgcgc ctgtggacct aagtctacgc agatcatatc catcgccacg cgcccgacaa
     8101 tcggtacttc gcgaccgttc accagcactg gcgtaccgga cggcgcggcg cgcggataac
     8161 catcgccata gcccatcgcg actacgccaa gacgggtatc acgttcgctt acccaagttc
     8221 caccataacc aacaggctct ccggctttat gctcacgcac ggcaatcagg ctggaggtta
     8281 gtgacatcac tggctgacag ccaaaatcgg caccggtgga gcgatcttcc agcggcgaga
     8341 cgccataaag aatgatgccc gggcgcaccc agtcaaaatg cgactgtggc cacagcagaa
     8401 tgccacccga cgcggcaatg gaacgttgac caggtttgcc ttcgcaaaag gtattaaaga
     8461 tagcgagttg tttctcggtt gcgccacatt ttggttcatc cgcgcgcgca aaatggctga
     8521 cgatattcac cggctgacga acgtttttgc actgggtcag gcgatgataa aacgcctcag
     8581 cctgttccgg ccttacgccc agacggtgca taccggtatc gagtttcatc cagacggtaa
     8641 ccggctcgtc caagctagcc tcttccagcg cagccagctg ttcttcgtta tgcacggcgg
     8701 tatgaaaatg ttgcgcagaa atcgtcggca gatctctggc atcaaaaaag ccttcgagta
     8761 acagtacagg tttggtgatt ccccccgcac gcagtcgcag agcttcttcg agacgggcta
     8821 cgccaaaggc gtcagcatcg gggagcgttc gcgcggtctc aagaagaccg tgaccataag
     8881 cgttcgcttt caccaccgca accattttac tggcaggtgc cagttcacga agacgttgca
     8941 ggttgtgtcg cagagcgcgg cggttaatca caacagttgc cgcttgcatt tgtgttcctt
     9001 gataattgtt tgctttaatt acctaattca taaaataatt attattcgtc gtcgtactgc
     9061 ggccccgcat agttgtcgaa gcgcgaccat tgaccgttaa aggtcaggcg taccgtcccg
     9121 attgggccgt tacgttgttt accgataata atttccgcga tgccttttaa atcactgttt
     9181 tcgtgataca cctcatcacg atagataaac atgatcaagt ccgcatcctg ctcgatagag
     9241 ccagattcac gcaggtcgga gttgaccgga cgtttgtcgg cacgttgttc cagagaacgg
     9301 ttcaactggg acagcgccac caccggcacg ttcagttctt tcgccagtgc tttcagcgag
     9361 cgggagattt ctgcaatttc cagcgtacgg ttatcggaaa gcgccggtac gcgcatcagt
     9421 tgcaggtagt cgatcatgat aagcccgatg ccgccgtgtt cacgggcaat acggcgtgcg
     9481 cgggaacgca cttccgttgg cgtcaggccg gaagagtcat cgatatagat attgcgtttt
     9541 tcgagcaaaa tacccatggt gccggaaatg cgcgcccagt cttcatcatc gagctgcccg
     9601 gtacggattt tagtctggtc aacgcgcgac agcgacgcca gagaacgcat catgatctgt
     9661 tctgatggca tctccagcga gaagataagt actggtttat cctgcaacat tgccgcgttt
     9721 tcgacgaggt tcatcgcaaa tgttgtttta cccatcgacg gacgcgcggc gacgatgatc
     9781 aaatccgacg gctgcaagcc agcggttttt ttgttgagat cgtcataacc ggtgtttacc
     9841 ccggtaacgc catcgtgtgg ctgctgaaac aactgctcaa tacgcgccac ggttgcgtcg
     9901 agcacatcgg cgatgttctt cggcccttcg tctttgtttg cacgactttc ggcaatttta
     9961 aagacgcggg attcagcaag gtccagcaga tcttcgctgg tacgcccctg cggatcaaaa
    10021 ccggcttcag caatctcatt cgcaaccgag atcatctcac ggacaacggc acgttcacgc
    10081 acgatgtccg cataagcact gatgttcgcc gcacttggcg tattttttga cagctctgcc
    10141 agataagcaa aaccaccgac gctatcgagt tgcccctggc gttccaacga ttccgcaagg
    10201 gtaatcagat cgataggact accgctttcc tgcaaacgcg ccatttcagt aaagatatga
    10261 cggtgtgggc gggtgtaaaa atcgtctgct accacacgct cggctacatc atcccagcgt
    10321 tcgttatcca gcattaaacc gcccaacacc gactgctccg cttcgatcga gtgcggaggc
    10381 actttcagcc cggcaacttg tggatcgcgt tcgcggggtt cagcctgctg tttgttgaag
    10441 ggtttatttc ctgccatagt gaatggagtt accgaaataa agaatgggtc gaaactttac
    10501 catatgaagc agaccctgac gatacgttct ggaggacaca tggcaacacg aattgaattt
    10561 cacaagcacg gtggcccgga agtacttcaa gccgtagagt tcactcctgc cgatccggcg
    10621 gagaatgaaa tccaggtcga aaataaagcc atcggcatca attttatcga cacgtatatc
    10681 cgcagcggcc tttacccgcc gccatcgcta cccagcggat taggtaccga agcggcaggc
    10741 atcgtgagta aagtcggcag tggtgtaaag catattaagg ctggcgatca tgtagtctat
    10801 gcgcagtcgg cgttaggcgc ttacagctct gtgcataaca ttaatgcgga taaagcggcg
    10861 attctgcctg cggcaatttc ttttgagcaa gctgcggcat ccttcctgaa aggcttaacg
    10921 gtttattatc tgctgcgcaa aacctatgaa attaaacccg atgaacagtt cctgttccac
    10981 gcagcggctg gcggcgttgg cttaattgcc tgccagtggg caaaagccct gggcgcgaaa
    11041 ctcatcggca ccgtaggaac cgcgcaaaaa gcgcagagcg cgctaaaagc cggcgcgtgg
    11101 caggttatta actatcgtga agagaatctg gtcgagcgat tgaaagagat caccggcggt
    11161 aagaaagtgc gcgtagtgta cgattccgtg ggcagagaca cctgggaacg gtcgctggat
    11221 tgcctgcaac gccgcggctt aatggtcagt tttggcaact catcaggtgc ggttaccggt
    11281 gtgaacttag gcattctcaa tcaaaaaggc tcgttgtatg tgacacgccc ttccctgcaa
    11341 ggctatatca ccacgcggga ggaattaacc gaggccagta atgaactgtt ctctttgatt
    11401 gccagcggtg tgattaaggt cgatgtcgcc gagcagcaga aatatccgct gaaggatgcg
    11461 cagcgtgcgc atgagattct ggaaagccgg gcgacgcaag gttccagcct gctgattcca
    11521 taaaagaaat agggcttcca cctgggaagc cctttctttt tatagttcgg ctgtatgtag
    11581 ggtacagcac gatgaatctg ttagaggcgc aataatgaca gatttgatta tcaattccta
    11641 ttttgttcta aggataaaac cttaggttgt gatcatccgc acaatccctt agtaacgcca
    11701 gcggtcataa cgctgatatt tcggcatttt tggtgcttta atcgccttaa taacccacac
    11761 caccgcaatc gccagtagta accacggcag caacttaatc atcaatgcca gcataccgcc
    11821 gaggaacata atggccgtcg ccacaaccag cgcggcgata atgcccagca acgaaacgcc
    11881 ggtgaccatc agcatgacaa aaaagccaat cacaaaaagt agttccagca tgatgctctc
    11941 ccaaatatga aatctcttgc tggcattaca agaatcatgc caaaaataat ctattgattt
    12001 aacagcaaaa cgccccgcga cggtgcgcag ggcgtggtga atttgactac tttttggtga
    12061 aaagttaacg cttatccgcc accagtttga gcgcgtgttc cagcacgtta atgtcagcac
    12121 ccgctttatg ggcattttca cttaaataac gccgccactg ccgcgcgcca ggaataccct
    12181 ggaacaaacc cagcatatgc cggataatat ggccgagata cgtcccctgg ctgagttcat
    12241 gctcaatgta cggatacatg gcacgcacta ctgccaccgg atcggcatcg gtatccgagg
    12301 aaccaaagat ctcccggtct accgccgcca gaatacccgg attctgatac gcctcgcgcc
    12361 cgaccatcac gccatccata tgttgcaaat gcgctttagc ttcttccagc gacttgatac
    12421 caccgttaat cgacattgtc agatgcggga agtcacgctt cagttgatac acacgcggat
    12481 aatcgagcgg cgggatttca cggttttctt tcgggcttaa ccccgaaagc caggctttac
    12541 gtgcgtggat gatgaacatc tcacactcgc ctttgccgga aacggtattg atgaaatcgc
    12601 agagaaattc atagctgtcc tggtcgtcga tgccaatacg cgttttcacc gtcaccggaa
    12661 tcgacaccac atcgcgcatc gctttcacgc agtcggcaac cagctgcgca ttacccatca
    12721 gacacgcacc aaacatgccg ttctgcaccc ggtcagacgg gcagccgaca ttcaggttga
    12781 tctcatcata tccgcgcgct tctgccagct ttgcacactg cgccagcgcc gccggatcgc
    12841 taccgcccag ttgcaacgct accggatgtt cttcttcact gtacgccaga taatcacctt
    12901 taccgtgaat aatcgcccct gtggtcacca tttcggtata aagcaacgta ttgcgggaaa
    12961 gcagacgcaa gaaatagcgg caatgtctgt ccgtccagtc gagcattggt gcaacactaa
    13021 accgaccact ccagtgaacg tcagtttttt caggcattgc gctggtttgg ttgatttttt
    13081 gcatttcaga attaccgtgc attttcaaat gtagagatta ttttatcgat atatcatggg
    13141 gttatgttat tcagcttcac tgttcaggag gctcaatagc ggggtactat accataacaa
    13201 caggaagcgc ctgtctcatt gcaaaagaaa attgagatcc tctcaaggca tgaagctctc
    13261 acgaagtgat ggaaataatc ttattagccg ttaactttgt taaggccaat gataaacaat
    13321 ccaggttcga cgataaataa aaaatcacac attaaactct ggtgatatat ctccctgcta
    13381 atagcattga tagagaaaaa agaacccaat aaatattggg tccttttata taatgccttc
    13441 catactatcg aagaacttca catattattt ctccgattta accccgaaca agtcataaat
    13501 taatttagaa gtgtctgtaa gtattttaat ctcttccttt gaggttgggt caaacgactt
    13561 cgcgaagcta attaattgtg gtgcagcatc tcgcattttg cttaaaatat caggatcgag
    13621 cgttccttca ttcaccagat gtgacatctt atccagatag ccatcaaaat tcatttctcc
    13681 cccatctgcc agacgcttca tctctcccag gtacttcttc atatcacgtt gatataattt
    13741 ttcaaactga gctttcaggt agctctcatt attttcatta atatacactg tttctgataa
    13801 atctgcgtag gcactagaat atgaaagtga aattaacagc gaagcaaata attttaagct
    13861 gttgtcatca tgttcctggc aggcattaac aaacgttaaa aaaccagaac ctattgcttg
    13921 aaaatgtata ttcgctaaag gttcattatc cttagattcc tcataaaata gctgaaccgt
    13981 ggagggaagg ccattcttag tttctacatc aaaagtacat tgttcaatgg gcattgcatc
    14041 tttattatcc ccaggattac atatactatt aatcgcgaaa tgaaaaatag cccggtcaac
    14101 aactgatgca ataaagtcac gatcgtttaa atcctcatca gtgcaatcgt cagtatactt
    14161 attgacccac attttgagat catttatttg ctcaattaaa aacacatttt ttacgtgcat
    14221 gccataaatt atgcgtgatt cgttgaataa ataaatctat ttttgcaata attatatccc
    14281 gatgacgcag cgctgcataa tcaatagatt taccatatat attatttaat ttacctttga
    14341 ttgaacctct attatccaat ttatctttgg ttgcatcttt attttcgaat tgatttgaat
    14401 tgagtgtttt attaaagtat ccagttgcac cgggtattat cttcaacatg attcatctac
    14461 cttttttgtc gtaccgtgtc ctccttataa ttctttttcc atctggtcac tatcaaaaat
    14521 cgctcataat ttctgaacta caggtatgcg tttaaaccca gttaaataaa tagcgagtta
    14581 acttgaagca atttatttaa aaatgaatta ttaattatta tcgtgttgat aatggtacga
    14641 caaggcaaca caacacctta cctacaaaac agaaaatcaa cattacggca acaataaggg
    14701 ttctcggtgt ttttcctgga acatggtaaa gtaaggacat tcttaacccc cactttgagg
    14761 tgcccgatgg aaaagaccac aacgcaggag ttattagcgc aggctgaaaa aatctgcgcg
    14821 cagcgtaatg tgcgcctgac cccacagcgc ctggaagtgt tgcgcctgat gagtctgcag
    14881 gatggcgcta tcagcgctta tgatctgctt gatttactgc gcgaagctga accgcaagcc
    14941 aagccgccaa cggtttatcg cgcgctggat tttctgcttg agcaaggttt tgtgcataag
    15001 gtggaatcca ccaacagtta tgtgctctgt catctgttcg accagcccac gcatacgtca
    15061 gccatgttta tttgcgatcg ctgcggcgcg gtgaaagaag agtgtgcaga aggcgtggaa
    15121 gacattatgc atacgctggc ggcaaaaatg gggtttgccc tgcggcataa tgtgattgaa
    15181 gcacatgggc tctgtgcggc atgcgtggaa gtggaagcgt gtcgtcatcc tgaacagtgc
    15241 cagcatgatc actctgtgca ggtgaaaaag aaaccgcgtt aagagggcgt acatccttgt
    15301 acacgtcggg caggagggat taattaccag cgatattcat tgcgggtttc ccaatccacg
    15361 acctcttttt ctgcctgatc tttctgataa ccataacgtt cctggatttt accgaccagt
    15421 tgatcacgtt taccttcaat gatcgtcata tcatcatcgg tcagtttgcc ccattgctct
    15481 ttcactttac ctttaaactg tttccagtta ccgccggctt catctttatt cataatcaag
    15541 acctcatcgt taggttgtga atgagagtac gttcactttt cttctgaacg tgagattaag
    15601 tgtagtcagc aatttggctt aggataattt attcagaatt tttaaccgtc acgttgcggc
    15661 aaaccaggta ccgttgcgcc agtgacgccg ccagatagcc gccagagaaa gcccgcgcaa
    15721 cgccagaaag acggttaatg ccagccacaa accatgatta cccagccacg gcagcgtaag
    15781 gagcgtcagc gcaaaacctg cggcggccac cgccatactg ttacgcattt cggcggcacg
    15841 cgttgcgcct ataaacatgc cgtccagcag ataacaccag acgccaacca acggcaaaat
    15901 cacctgccag ataagatagc ggtcagccag ctgctgaatc tgggttaacg acgtcagcaa
    15961 cgcaatgatg tgttcccctg ccagcaaata aaccaccgaa aacagtaacg ctacaatccc
    16021 cgactggcgg cacgctgccc gccatacatc cagcaactgg ctaccgtcgc gcgcaccgta
    16081 tgcctgaccg gagtgcgctt caaccgcgta ggcaaaacca tcaagcgcat aggcggtaaa
    16141 ggtgagtagc gtcatcagaa ccgcgttaac agcgataatg tcactcccca gtcgcgcacc
    16201 aagtacggtg atcgcgccga aacagagttg caacaacagc gaacgcagca tgatatcgcg
    16261 gttaagcgcc agcaagcgac ggaagtttcc tcgccaggca gttttcagca tttcgccgga
    16321 aattccgcgt agtttgagga ttttacgcac cattagcaga ccaatcagca atgttgcata
    16381 ttccgcaata accgtcgcca gcgccgcgcc ctgcacgttc atatgcagcc ccatcaccag
    16441 ccagacatcc agcacaatgt tgaggatatt gccgaccact aacaaaatta ctggcgcacg
    16501 ggcatattgc acgccgagta accaaccgag taataccaga ttcgccagcg acgccggagc
    16561 gcttaaccag cggatttcaa gaaagcgccg cgcctgttcc aggaccgctt cactaccgcc
    16621 aacaatatgc agcgccagat cgataatcgg cgtacgcaac agcgcaatta acgccccggc
    16681 ccccaacgcc aacagcaacg gttgcaccag cgcacgggct aatgcctgag gatttttggc
    16741 accataagcc tgcgcagtca gcccggtggt gctcatgcgt aaaaacagca acagcataaa
    16801 gagaaagctg gtcgccgttg caccaaccgc cacgccgccc aaataaaccg gactatcaag
    16861 atgaccaatt accgccgtat cgaccagtcc cagcaacgga acggtgatat tggagaaaat
    16921 catgggtaag gcgagatgcc agagtgcttt atcagatgaa gtgaggaatg ccatgcagac
    16981 aagcctgatg aagagagatg aaaaacaaac cgcgacgcca ggcggcatcg cggtctcaga
    17041 gatatgttac agccagtcgc cgttgcgaat aaccccaacc gccagccctt caatggtgaa
    17101 gctctgctga cgaaggtcaa cgacaattgg tttaaactcg ctattttctg gcaacagttc
    17161 gactttattg ccctgttttt tcaggcgctt aacggtaact tcgtcatcaa tacgtgcgac
    17221 aacgacctga ccgttacgta catcctgagt tttatgcact gccagcaagt cgccatccat
    17281 aatgccgata tctttcatcg acatcccgct gacgcgcagc aggaaatcag cattcggctt
    17341 gaacaaggaa ggatcgacct gataatgacc ttcaatatgc tgttgcgcca gaagcggttc
    17401 accggcagcc acacgaccta ccagcggcaa cccttcttcc tcttcctgca acagacgaat
    17461 cccgcgtgat gcgccggaaa caatttcaat aacgcctttg cgtgccagcg ccttcagatg
    17521 ttcttcagcc gcgtttgggg aacggaaccc caaacgctgc gcgatttccg cacgcgtcgg
    17581 cggcatacct gtctggctga tgtgatcacg gatgagatca aacacctctt gttgcctggc
    17641 cgttaacgct ttcattccgc cccctgggtg tatatacagt tatactgtga gtatatacag
    17701 caaaaggcga ttttggaacc ataaactgca caataaacca gagatttatc gaaatctgga
    17761 agggttatcc aaaatgcgac cataacagga tgcaccaggt aatcacggcg acgataatgg
    17821 caatcagcac cgcggcggat cccatatctt ttgcgcgtcc ggaaagctca tggtattcag
    17881 agccaattcg gtcaaccact gcttcgatgg cgctattgag gatttccaca atcatcacca
    17941 gcatcacgga gctgataagc agcacgcggg taattgcgtc cacatccagc cagcaggcga
    18001 tgaccaccgc caacaatacc gctacgcctt cctgacggaa tgccgcttcg ttgatccatg
    18061 cagcgcgtaa acctttccag gaatagccag cagctttgat aattcgggtg aatccagtgg
    18121 tattattgac cattgaaaga acctttttac attatgagcg tcaatatcag tgtaccgtta
    18181 aatccgatga ttcgggtttg cgtaacgccg cgcgaaacat gagcggatac cacagaattt
    18241 cccatgactt tctgctatcc ttgccgcgca tttgcattat taaccagagg ctttacatcg
    18301 tttatgtccg gctggccacg aatttactac aaattactga atttaccatt aagcatcctg
    18361 gtaaaaagca agtctattcc ggcagatcct gccccggaac tggggctgga tacctctcgt
    18421 ccaattatgt acgttttacc gtacaactcg aaagcagatt tgctgacgtt gcgcgcccag
    18481 tgtctggcac atgatttgcc tgacccgtta gagccgctgg aaatcgacgg cacgctactg
    18541 ccgcgctatg tgttcattca cggcgggccg cgtgtattca cctattacac gccgaaagaa
    18601 gagtctatta agcttttcca cgactatctc gatttgcacc gtagcaaccc aaatctggat
    18661 gtgcagatgg tgccagtatc ggtgatgttt ggtcgcgcgc cggggcgtga aaaaggcgaa
    18721 gtgaacccgc cgctgcgtat gcttaacggc gtacagaaat ttttcgctgt actgtggctc
    18781 ggtcgcgaca gttttgtgcg tttctcgccg tcagtgtcgc tgcgccgtat ggcggatgaa
    18841 cacggcacgg ataaaactat cgctcagaaa ctggcgcgcg tggcgcgtat gcactttgcc
    18901 cgtcaacgtc tggctgccgt aggcccacgt ctgcctgctc gtcaggatct gtttaataag
    18961 ctgctcgcct cccgcgccat tgccaaagcg gtagaagatg aagcgcgcag caaaaaaatc
    19021 tcccatgaaa aagcgcagca gaacgcgatt gcgctgatgg aagagattgc ggcgaatttc
    19081 tcttacgaga tgatccgcct gactgaccgc attctgggct tcacctggaa ccgattttat
    19141 cagggcatca acgtccataa cgccgagcgc gttcgccagc tggcccacga cggtcatgag
    19201 ctggtgtatg tgccttgcca ccgcagtcac atggactacc tgctactttc ttacgtgctg
    19261 taccaccagg ggctggtgcc gccgcatatc gccgccggga tcaacctgaa cttctggccg
    19321 gccgggccga ttttccgccg tctgggggcg ttctttattc gccgtacttt taaaggcaat
    19381 aaactttatt ccaccgtttt ccgtgagtat ctcggcgaac tgttcagccg tggttattcc
    19441 gtcgagtact tcgtggaagg cggtcgttcc cgcacggggc gtttgctgga tccgaaaacc
    19501 ggtacgctgt cgatgaccat tcaggcgatg ctgcgtggcg gcacgcgtcc gattacgctg
    19561 attccgatct atatcggtta tgagcacgtc atggaagtgg gtacttacgc caaagaactg
    19621 cgcggcgcga cgaaagagaa agagagcctg ccgcagatgc tgcgcggttt aagcaagctg
    19681 cgtaatctcg gtcagggtta cgtcaacttc ggtgaaccaa tgccgttgat gacctacctt
    19741 aaccagcacg taccagactg gcgtgaatct atcgatccca tcgaagcggt gcgtccggcc
    19801 tggttaacgc cgacggtcaa taatattgct gccgatctga tggtacgcat taacaacgca
    19861 ggcgcggcaa acgccatgaa cctgtgctgt actgcgctac tggcatcacg tcagcgctca
    19921 ctcacccgcg agcagttaac cgagcaactc aactgctacc tggatctgat gcgcaacgta
    19981 ccttactcca cggactctac cgttccttca gccagcgcca gcgagcttat cgatcacgcg
    20041 ctgcaaatga acaagtttga agtcgagaaa gacactatcg gcgacatcat cattctgcca
    20101 cgcgagcaag cggtgctgat gacctactat cgcaacaaca ttgcgcatat gttggtgctg
    20161 ccttcgctga tggcggcaat tgtcacccag catcgccaca tctcccgcga cgtattgatg
    20221 gagcacgtca atgtgcttta cccaatgctg aaagcggagc tgttcctgcg ctgggatcgc
    20281 gacgagttgc cggacgttat tgatgcgctg gcaaatgaga tgcaacgtca ggggctgatt
    20341 accctgcaag atgatgagtt gcatatcaac ccggcgcatt ctcgcaccct acagctgctg
    20401 gccgcaggcg cgcgcgaaac gctgcaacgt tatgccatca ccttctggtt gttgagtgcc
    20461 aacccgtcga tcaaccgcgg tacgctggag aaagagagcc gcaccgttgc gcaacgtctc
    20521 tccgtgctgc acggcatcaa cgcgccggag ttcttcgaca aggcggtgtt cagttctctg
    20581 gtgctgacgc tgcgtgatga agggtatatc agcgatagcg gcgatgccga accagcagaa
    20641 acgatgaagg tttatcagtt gctggcggag ttgattacat cagacgtgcg tttgacgatt
    20701 gagagtgcga cgcagggcga agggtaatca gagagaattg ccggatgcgg cgaaaacgcc
    20761 ttatccggcc taccatgacc tgcaaattca ataaattgcg attcaccggg taggcctgag
    20821 aagcgcagcg catcaggcaa cccagaagaa agccggatga tcatccggct tttttacatc
    20881 atcagaaatg ccagtaactc attgccagtc ctaaaaacag taccagacca acatagttat
    20941 tattcataaa tgctttaaag caggcttcac gctcgcggtt ggcaatcagt ttttgttgat
    21001 aaacaaacag cgcgctagcc accacaattg accagtaata tccccagcct aagccattta
    21061 actcaccgat gatcgccatc agtgccagta cgccaatctg caaaatacca ataatcaatt
    21121 tatcgtattg gccgaacagg attgccgtgg atttaatgcc aatcttcaca tcatcatcgc
    21181 ggtcaaccat cgcatactgc gtgtcgtaag ccaccgccca gagaatattg gcgaggaaca
    21241 ttaaccagca actcaatggc accgactcac tcacagcggc aaaagccatt ggaatcgacc
    21301 agccaaacgc cgcgcccagc accacttgcg ggagatgggt ataccgcttc ataaacgggt
    21361 acacccacgc caatgccaga gcggcaatcg acaacagaat ggtcatcgta ttcagcgtca
    21421 gaaccagtaa aaacgaaatc agtaccagca ctacaaacag cgcgcgcgcc tctttctctg
    21481 ttaccgcgcc gctgggaagt ggtcggttcg ccgtgcgctt aacatgacca tcaaacttgc
    21541 gatcagcata atcattcacc acgcaaccag cagcgcgcat cagccagaca cccgcgacaa
    21601 acaccgccag gatccagagc tgaggaacgc ccggtgtcgc cacccacaac gcccataatg
    21661 ttggccagag cagcagtaac gcgccaattg gcttatccgt acgcattaag cgatgaaacg
    21721 ccagcagctt attctgcgtc agactccact ccatattttt ttcctcttag tacaacggtg
    21781 acgccggtaa aaacagttct gttagcaaca gcggtttacc gcttaatcgc aggcgggaac
    21841 gtcgccccca cagtccggca tcacggccta tctcgataaa atcccgggtt agtgtcgatg
    21901 atgtgaacag atagcgtcct aacggcgttt tacccaattt ttgtaacgct agttccggcc
    21961 cgcttaatgt tgatacagga acgacggtac gaccggcaag ccacggttca ccatcggcac
    22021 ataacaaaat ttcacgtaac cagtaacgag actctttcgg cagtagcggc agttcttcgg
    22081 ggatttcatt ctgctcgaca aacccctcgc ggatcatcgt cacactcacc gtttttccct
    22141 gctgttcaaa acgttttgtc atggaatcct ccagcaacag ccagtcgaac agttgcggat
    22201 ccagggcagg gatctcttta aaatagcgca gcgcacgcag ttgcgttaac gcggggtgtg
    22261 acatgccgaa ctctccgtta cataacgtaa aggcattgta tcgcagaaaa gtgtttacgg
    22321 gagatgtaaa gcaaattaag tgatctaatg cgcaacaaaa acgcaacaat cgcatgaagt
    22381 ggtaaaacgc tgttgaacct tacgctttgc cgatgcccgt gctggcgagg gcaaccatag
    22441 gggcatcacc gcccatgatg ctaggaacgg tgatgttttc ctttacttaa atacctttat
    22501 tctttatacg agtagctcgc caactcgcag taaaaattgg gaaaagcgca tatttaccat
    22561 gagatttgga gctgaaaagt gtttcctgag taagattaat aggcaacatc ttatgtctgg
    22621 atttatagat acacgtggaa ttccaattgg tatcttttgt aggattcaac atatcatgta
    22681 agcgctggga gcttaccacc ataacaggcc catcatcgac agaggagaac aggataaata
    22741 tattatcgtt tcgtgttaat ttattagtgt taaaggttct taacttttct tcagtttcta
    22801 taaatatagg tgaaacatca tcactacgaa tccactcttt ataaagatac caggcatttg
    22861 ctttgatcat atcatccgtt gatcgtaagg cctgatcgat aagttggata aaaaaattgt
    22921 tatttttatc aaccagccct ttattcatat taaatctgtt aataaaatgg atcatctgtt
    22981 gggtatcgaa attcacctta ctgaatcgat gttgacgcat aaaattatcg aacttgtcat
    23041 cttccccaga atgtatataa ctacgtataa ttggatcttt tatataagga caatggctaa
    23101 atgttttctt catattctca ataaagtttt tattaacaat attgagaatt tttaattgca
    23161 tcaccagttt tactgccaga acggatttca atatcattgc attatctggc aatgtatcaa
    23221 tcatttttat aatgttattg atataaccat cgagatcttc attcccaaat atatctgaat
    23281 aatcaatagt taattccaat tgttctgtaa ttcaatctta aaaattccct gtcgacattc
    23341 gcacatgatc aaacataaca gctgtcaaat tcgatttaat aaatgatatt tctttggcag
    23401 acagaaactc tgctctggtg aagtgcgaag cgtaaagata acaattatcg aagtttgaat
    23461 ttctaatctg acattcgcga aaattactat gaccaagttg actctgcgag aagtcaactg
    23521 tatcaagaat attttggcta aaatcaacac attcgccact cgttttgaga aaaatcacat
    23581 tgtgtaaacg tcgacattta aatatgacat tatgaactgt tgccttagta aaattgcaat
    23641 tctcaatttc tttacattcg aaagttacat gttgtagatt tgatgataaa aaagacacct
    23701 tagaaataac gtcaccttta aaatgacaat ttgttaaatc aaaatcagac aggtcgatat
    23761 acccctgcgc atctttatcc atcagaaaat tacaagcgca ttctattttt tcatttctgt
    23821 agaattttac acttatagta tcattactaa ttgagacttc acgtaacata tttattgcat
    23881 gatttttttc gttccgttgt tttatattta aaaatatatc actgatataa atgaagttat
    23941 ctctgtttaa acctgtcacc ccattgaaat tacgtagtaa atcaagatct actatattat
    24001 ttccttgcac cacatcatgt ttcatcaaag agttattgcc gccagaattt gtgatatgac
    24061 ctatcataaa acctctgtaa tacagaaata aaataacctg gaaaagtagc ctgagcgtcc
    24121 agacactacc ggcgaaagga aaagaatctc tgataatgca ttgaaataat ggacattctt
    24181 agtgaagaag aagtgaagta ctataaaaaa tcgctcatat cccaggaagg aagcataaaa
    24241 aaaggtgcgc caggagacgc accagttgtt gcaaaatcag cactgtgggg cgtaattacc
    24301 ccttgccttt tacactgctg ataaaggtgg aacgggcaga tgtcgatccc aggcgttcag
    24361 cttcatcaag cagttttaac gccttatcaa catcaccttt cgcgacagcg tttttgatcg
    24421 cggtattaaa ataactttcc gtgtcgttga gcatcggctc gcttttcttc accggcaccg
    24481 gagcaggtgc agccacagct ggtgccgccg tgttgcctac cgtaaccgga gctggagcgg
    24541 aagaaccaaa tagtggtcct accaacacgc tggagctgga gttcgttttg actttcagtt
    24601 tcagtaagcc atcgctggta tgacgagcaa ccggatcggg aatatccggg atcgagttac
    24661 cgacgccttt ggcataggct ttagccgggt cgagcagttg ggtcgtctgc tggagatctt
    24721 tttccatggt aaagaccaga acataaagtt tttgctgccc caacgccggt gtcaggcgca
    24781 taacgccttc cagccgatct gcactcatca cgcctagttc ctggtaggtg aaataactgc
    24841 tggggaagaa ggctgatggg gtcatgttct gatcaataat cagcacgttc ggcgcaaaaa
    24901 cgctggtttg tttgttcact tcgctggtca gcgtcagggt cagttcgcca atgtttgccg
    24961 ggacgctgta cgcagcaacc ggaccactga tgccgggaac gttcagttgt tggccgccgg
    25021 tcgccagttg ggtggtctgg attttagatt gatcgaccgg tgtccaggtg agttgttgca
    25081 gcgcggcaga tggaatggct ggcgcgtcgc tggtgttttg cggtacgtag ttaacatcgg
    25141 caaggctaat tccaggcgcg cttgccagta accctgctga taaacagagg gcgatgagac
    25201 ttttattcat tttcattgtt ttcacctcaa aatctggagc tcagcggtag ccaggcaata
    25261 gcgcgctaaa cccgataatc agaggggctt tcgccccttt cagataatga caacctgttt
    25321 ttatgccgga tgcggcgtaa acgccttatc cggcccaggt tttgctatta ccaccagatt
    25381 tccatctggg caccgaaggt ccactcgtcg ctgtcgccac gaccgaagct gccgccgttg
    25441 aaatcagcag gaacggcttt gccgtagttc gggttaacct tgctatcgcc gttgtagtcg
    25501 taaccccatt tctcatccca cttggcgtag gttgcgaaga cacgaatagc cgggcgtgac
    25561 cagatgctgt cgccagcctg ccattgttgt gcgagggtaa ttttgtactg attgttcttg
    25621 tcgccggtgc gctgggattc gacgttgtcg tagccgattt ccatcacggt gctcatgatt
    25681 ggcgtccact tgtacatcgg gcgaatacca acggtccacc acttggtgcc gttgtcgtta
    25741 tcccagttga tatcctggta catacccacg tacatcatgt cccagttgtc gcccatggag
    25801 atcgcaccgt ggtcgaggat acgcagcatg tgaccgttgt tgttgatatt gtaggcaaat
    25861 ttttcgttat caaacgcgac gccagaaccc tgcgacagac ctttaccctg cgaggtcatc
    25921 gagtcagtag cgtactgaac aacaaacttg ttaaagccct tcaggacact ctgagtatgt
    25981 tcagcagtga ataaccagcc gtctttcgat gcgccatcaa ccagacgata gttatcacgc
    26041 aggttggcac gaccgtagtc gacacccagt tctaatgtgc cgcccgggtt gatttccatc
    26101 tgcgctaaac gcacatcgaa aacgtcgttc gcggtttcgt tggtatagtc ataaatattg
    26161 ttgctggcaa aagaggaaga accaccagct tcagaggagc gggttgctgc cagagagagt
    26221 ttaccgaagc caacatcgat gttttccaga ccggcaccag gaccagaaat atcccagtag
    26281 tagaagtcga tcatatgaac gtcatgacgt tggtagaagc gcttacctgc ccagatggtg
    26341 gaacctggca gccattcgat caggtttttt ccctgcacgt ttgcttcacg gaaggccgga
    26401 tcggtagctt cccagtcatt ctgttgcgcg acggaatagg ccacgttagt gtcgaaatag
    26461 aagctcttat cgccctcttt ccacacttcc tgacccaatt ttaattcagc ataagtttca
    26521 cattcgttgc caagacggta tttactttga gcaccggtag tctggaaaca ctgttgttca
    26581 ccgccgctac ctgtccagcc aataccggaa cgtgcatagc cgtggaaatc aacagccatt
    26641 gcctgagcag acattacgcc cgctgcgacg gcaaccgcca gaggaagttt gcgcagagta
    26701 atcatcattc tatctcctga gtcattgctt ttcttttttc acatcacctg tgacaggctt
    26761 tgtgtgtttt gtggggtgct taaacacccg gctccttatg cagtcgacga catgcagtgc
    26821 catcctcacg gaacagatgg caacgctctg gcggcaggcc gatagcgaat gtggcacctt
    26881 cttctaccaa caccacgtcg ttctggcggt acaccaggtt ttgacgaatg gaagggatct
    26941 ggatatagat ttgggtttcg ttgccgagtt gctcgacgac ctgaacttca ccctcaagga
    27001 tgacgtcagc gatatcactc ggcagtagat gttccgggcg aatacccagc gacatattgg
    27061 ctccaacctg gacatcacgg ctttcaacag gcagccagac ttgttgacga tttggcatcg
    27121 gcagctccac ctgcacttga tcgattgcgg tggcggtcac ttttaccggc aggaagttca
    27181 tctttggcga accgataaat ccggcgacaa aacggtctgc cggatagtgg tacagttcca
    27241 gcggtttccc aacctgtgcc acgcgaccgg cgtccagcac cacgattttg tcggccagcg
    27301 tcatcgcttc gacctgatcg tgggtgacgt aaatcattgt gcggcccagg cgtttatgca
    27361 gacgggagat ttcgatacgc atttgcacac gcagtgcagc atcgaggttg gagagcggtt
    27421 catcgagcaa aaatacgctt ggctcggcca ccagcgtacg gccaatcgcc acgcgctgac
    27481 gctgaccacc ggagagcgct ttcggtttgc gatccagcaa atgcgccagt tgcagcactt
    27541 ccgccacctg gttgacgcgt tggttaatca cctctttttt tgcgccagcc agtttcaggc
    27601 caaatgacat gttttctgct actgacaggt ggggatagag cgcgtaagac tgaaacacca
    27661 taccaacgcc gcgttctgct ggcggagtgt cattcatccg tttctcaccg atgaacaggt
    27721 cgccgctggt gatcgtctca agcccggcaa tcatgcgcag taaagtcgat ttaccgcagc
    27781 cagacggtcc gacaaacacc acgaattcac cttcatggat atcgagattg atatctttcg
    27841 ataccacgac ctcgccccag gctttcgtta cattttgcag ctgtacgctc gccatgccct
    27901 tctccctttg taacaacctg tcatcgacag caacattcat gatgggctga ctatgcgtca
    27961 tcaggagatg gcttaaatcc tccaccccct ggctttttta tgggggagga ggcgggagga
    28021 tgagaacgcg gcttctgtga actaaaccga ggtcatgtaa ggaatttcgt gatgttgctt
    28081 gcaaaaatcg tggcgatttt atgtgcgcat ccccacatta ccgccaattc tgtaacagag
    28141 atcacacaaa gcaacggtgg ggcgtagggg caaggaggat ggaaagaggt tgccgtataa
    28201 agaaactaga gtccgtttag gtgttttcac gagcacttca ccaacaagga ccatagatta
    28261 tgaaaataaa aacaggtgca cgcatcctcg cattatccgc attaacgacg atgatgtttt
    28321 ccgcctcggc tctcgccaaa atcgaagaag gtaaactggt aatctggatt aacggcgata
    28381 aaggctataa cggtctcgct gaagtcggta agaaattcga gaaagatacc ggaattaaag
    28441 tcaccgttga gcatccggat aaactggaag agaaattccc tcaggttgcg gcaactggcg
    28501 atggccctga cattatcttc tgggcgcacg accgctttgg tggctacgct caatctggcc
    28561 tgttggctga aatcaccccg gacaaagcgt tccaggacaa gctgtatccg tttacatggg
    28621 atgccgtacg ttacaacggc aagctgattg cttacccaat cgctgttgaa gcgttatcgc
    28681 tgatttataa caaagacctg ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc
    28741 tggataaaga actgaaagcg aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt
    28801 acttcacctg gccgctgatt gctgctgacg ggggttatgc gttcaagtat gaaaacggca
    28861 agtacgacat taaagacgtg ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc
    28921 tggttgacct gattaaaaac aaacacatga atgcagacac cgattactcc atcgcagaag
    28981 ctgcctttaa taaaggcgaa acagcgatga ccatcaacgg cccgtgggca tggtccaaca
    29041 tcgacaccag caaagtgaat tatggtgtaa cggtactgcc gaccttcaag ggtcaaccat
    29101 ctaaaccgtt cgttggcgtg ctgagcaccg gtattaacgc cgccagtccg aacaaagagc
    29161 tggcgaaaga gttcctcgaa aactatctgc tgactgatga aggtctggaa gcggttaata
    29221 aagacaaacc gctgggtgcc gtagcgctga agtcttacga ggaagagttg gcgaaagatc
    29281 cacgtattgc cgccaccatg gaaaacgccc agaaaggtga aatcatgccg aacatcccgc
    29341 agatgtccgc tttctggtat gccgtacgta ctgcggtgat caacgccgcc agcggtcgtc
    29401 agactgtcga tgaagccctg aaagacgcgc agactcgtat caccaagtaa tgctgtgaaa
    29461 tgccggatgc ggcgtgaacg ccttgtccgg cctacaaaac tgaaacgcat gtaggcctga
    29521 taagacgcat cagtgtcgca tcaggcagtt gttgtcggat aaggcgtgaa agccttatcc
    29581 gtcctggaat gaggaagaac cccatggatg tcattaaaaa gaaacattgg tggcaaagcg
    29641 acgcgctgaa atggtcagtg ctaggtctgc tcggcctgct ggtgggttac cttgttgttt
    29701 taatgtacgc acaaggggaa tacctgttcg ccattaccac gctgatattg agttcagcgg
    29761 ggctgtatat tttcgccaat cgtaaagcct acgcctggcg ctatgtttac ccgggaatgg
    29821 ctggaatggg attattcgtc ctcttccctc tggtctgcac catcgccatt gccttcacca
    29881 actacagcag cactaaccag ctgacttttg aacgtgcgca ggaagtgttg ttagatcgct
    29941 cctggcaagc aggcaaaacc tataactttg gtctttaccc ggcgggcgat gagtggcaac
    30001 tggcgctcag cgacggcgaa accggcaaaa attacctctc cgacgcgttt aaatttggcg
    30061 gcgagcaaaa actgcaactg aaagaaacga ccgcccagcc cgaaggcgaa cgcgcgaatc
    30121 tgcgcgtgat tacccagaat cgtcaggcgc tgagtgacat taccaccatt ctgccggatg
    30181 gcaacaaagt gatgatgagc tccctgcgcc agttttctgg cacgcagccg ctctacacac
    30241 tcgacggtga cggcacgttg acgaataatc agagtggcgt gaaatatcgt ccgaataacc
    30301 aaattggctt ttaccagtcc attaccgccg acggcaactg gggggatgaa aagctaagcc
    30361 ccggttacac cgtgaccacc ggctggaaaa actttacccg cgtctttacc gacgaaggca
    30421 ttcagaaacc gttcctcgcc attttcgtct ggaccgtggt gttctcgctg atcactgtct
    30481 ttttaacggt ggcggtcggc atggttctgg catgtctggt gcagtgggaa gcgttgcgcg
    30541 gcaaagcggt ctatcgcgtc ctgctgattc tgccctacgc ggtgccatcg ttcatttcaa
    30601 tcttgatttt caaaggattg tttaaccaga gcttcggtga aatcaacatg atgttgagcg
    30661 cgctgtttgg cgtgaagccc gcctggttca gcgatccgac caccgctcgc acgatgctga
    30721 ttatcgtcaa tacctagctg ggttatccgt acatgatgat cctctgcatg ggcttgctga
    30781 aagcgattcc ggacgatttg tatgagcctc agcaatggat ggcgcaggtc cgttccagaa
    30841 cttctttaag attacgctgc cgctgctgat taaaccgctg acgccgctga tgatcgccag
    30901 cttcgccttt aactttaaca acttcgtgct gattcagctg ttaaccaacg gcggcccgga
    30961 tcgtcttggc acgaccacgc cagccggtta taccgacctg cttgttaact acacctaccg
    31021 tatcgctttt gaaggcggcg ggggtcagga cttcggtctg gcggcagcaa ttgccacgct
    31081 gatcttcctg ctggtgggtg cgctggcgat agtgaacctg aaagccacgc gaatgaagtt
    31141 tgattaaggg agataacaaa aatggcaatg gtccaaccga aatcgcaaaa agcacgtttg
    31201 tttattactc acctgctact gctacttttt atcgcggcga ttatgttccc gctgctgatg
    31261 gtcgtcgcta tctcgctgcg tcagggaaac ttcgcgaccg gtagcctgat cccggagcaa
    31321 atctcctggg atcactggaa actggcgtta ggttttagcg ttgaacaggc tgatggtcgc
    31381 attacgccac cgccattccc ggtactgctg tggctgtgga actcggtaaa ggtcgctggg
    31441 atttccgcga ttggcattgt ggcgctctcc accacctgcg cctacgcttt cgcccgtatg
    31501 cgctttccag gcaaagcgac gctgctgaaa ggaatgctga ttttccagat gttcccggca
    31561 gtactttcac tggtcgcgtt gtatgcgttg tttgatcgtc tgggtgagta cattccattc
    31621 attggcctga atactcacgg cggcgtaatt ttcgcgtatc tgggtgggat tgcgctgcat
    31681 gtctggacca tcaaaggcta tttcgaaacc atcgacagtt cgctggaaga agcggctgcg
    31741 ctggatggtg cgacaccctg gcaggccttc cgccttgtcc tgttgccgct gtcagtaccg
    31801 attctggcgg tggtattcat cctgtcgttt atcgctgcca ttactgaagt tccggtcgcg
    31861 tcgctgttac tgcgtgacgt aaacagctac accctggccg tggggatgca gcaatacctc
    31921 aacccgcaaa actacctgtg gggtgacttt gccgccgctg ccgtgatgtc tgcattaccg
    31981 atcaccatcg tcttcttgct ggctcaacgc tggctggtca acggcctgac ggcaggtggt
    32041 gtgaaaggtt aaagatgttg ttctgccaat gttatgccgc tgcaccctca acttacgtta
    32101 tcccaacttg tgactattat tcggcgctcc acggagcgcc tttttttctt tcgtctgcaa
    32161 tctgaatcgt tcgccggtta atattcccat catagaactt attattttta cgttatttgt
    32221 tttcccactt acgataattc tctttcgtgc tctgagtcac ggcaatagta ttgttttcat
    32281 caatttttga taattatcac aattaagatc acagaaaaga cattacg
//
LOCUS       DAETPZ010000042        31689 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.042, whole genome shotgun sequence.
ACCESSION   DAETPZ010000042 DAETPZ010000000
VERSION     DAETPZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.042"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(130..1650)
                     /locus_tag="K0W59_002175"
     CDS             complement(130..1650)
                     /locus_tag="K0W59_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI3023199.1"
                     /translation="MRTVITYLRFSSATQGAEGADSTRRQNELFKQWLRKNSDAQVVA
                     SFNDEGLSGYKGKHLSGQFGDMLSRIESGEFPEGTILLVEAIDRIGRLEHLETEALMN
                     RIIAHGIEIHTLQDGLIYTRDALSDDLGISIIQRVKSYIAHQESKQKSFRVSQKWEQR
                     AKLALAGEQRLTRNVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQANNI
                     SSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEAAIDAATFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKSKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDTMKRKASLTDKELPQLKDIDLTTKAGRVECQLI
                     LSKHLKGLTLGKGSVTITLQNDTEITIPTDPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            1741..2472
                     /gene="tonB"
                     /locus_tag="K0W59_002176"
     CDS             1741..2472
                     /gene="tonB"
                     /locus_tag="K0W59_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HBI3023200.1"
                     /translation="MPLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     KPKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRAL
                     SRNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWR
                     YEPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(2512..2910)
                     /gene="yciA"
                     /locus_tag="K0W59_002177"
     CDS             complement(2512..2910)
                     /gene="yciA"
                     /locus_tag="K0W59_002177"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HBI3023201.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3015..3554)
                     /locus_tag="K0W59_002178"
     CDS             complement(3015..3554)
                     /locus_tag="K0W59_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HBI3023202.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAVALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(3584..4327)
                     /locus_tag="K0W59_002179"
     CDS             complement(3584..4327)
                     /locus_tag="K0W59_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HBI3023203.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVCALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(4355..4444)
                     /locus_tag="K0W59_002180"
                     /pseudo
     CDS             complement(4355..4444)
                     /locus_tag="K0W59_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            4684..5322
                     /gene="ompW"
                     /locus_tag="K0W59_002181"
     CDS             4684..5322
                     /gene="ompW"
                     /locus_tag="K0W59_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HBI3023204.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGS
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(5382..5888)
                     /locus_tag="K0W59_002182"
     CDS             complement(5382..5888)
                     /locus_tag="K0W59_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBI3023205.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(5934..6434)
                     /locus_tag="K0W59_002183"
     CDS             complement(5934..6434)
                     /locus_tag="K0W59_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBI3023206.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLPRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(6520..6699)
                     /locus_tag="K0W59_002184"
     CDS             complement(6520..6699)
                     /locus_tag="K0W59_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HBI3023207.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSDKS"
     gene            complement(7080..7886)
                     /gene="trpA"
                     /locus_tag="K0W59_002185"
     CDS             complement(7080..7886)
                     /gene="trpA"
                     /locus_tag="K0W59_002185"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HBI3023208.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(7886..9079)
                     /gene="trpB"
                     /locus_tag="K0W59_002186"
     CDS             complement(7886..9079)
                     /gene="trpB"
                     /locus_tag="K0W59_002186"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HBI3023209.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(9091..10452)
                     /gene="trpCF"
                     /locus_tag="K0W59_002187"
     CDS             complement(9091..10452)
                     /gene="trpCF"
                     /locus_tag="K0W59_002187"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HBI3023210.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(10453..12048)
                     /gene="trpD"
                     /locus_tag="K0W59_002188"
     CDS             complement(10453..12048)
                     /gene="trpD"
                     /locus_tag="K0W59_002188"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HBI3023211.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(12048..13610)
                     /gene="trpE"
                     /locus_tag="K0W59_002189"
     CDS             complement(12048..13610)
                     /gene="trpE"
                     /locus_tag="K0W59_002189"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HBI3023212.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALHITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(13632..13724)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            13883..14764
                     /gene="yciV"
                     /locus_tag="K0W59_002190"
     CDS             13883..14764
                     /gene="yciV"
                     /locus_tag="K0W59_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HBI3023213.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            14761..15381
                     /locus_tag="K0W59_002191"
     CDS             14761..15381
                     /locus_tag="K0W59_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBI3023214.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKS
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            15482..16354
                     /gene="rluB"
                     /locus_tag="K0W59_002192"
     CDS             15482..16354
                     /gene="rluB"
                     /locus_tag="K0W59_002192"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HBI3023215.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(16394..16984)
                     /gene="cobO"
                     /locus_tag="K0W59_002193"
     CDS             complement(16394..16984)
                     /gene="cobO"
                     /locus_tag="K0W59_002193"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HBI3023216.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(16981..17739)
                     /locus_tag="K0W59_002194"
     CDS             complement(16981..17739)
                     /locus_tag="K0W59_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HBI3023217.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            17959..19008
                     /gene="sohB"
                     /locus_tag="K0W59_002195"
     CDS             17959..19008
                     /gene="sohB"
                     /locus_tag="K0W59_002195"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HBI3023218.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     AWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(19044..19295)
                     /locus_tag="K0W59_002196"
     CDS             complement(19044..19295)
                     /locus_tag="K0W59_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HBI3023219.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            19675..22272
                     /gene="topA"
                     /locus_tag="K0W59_002197"
     CDS             19675..22272
                     /gene="topA"
                     /locus_tag="K0W59_002197"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBI3023220.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVIDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            22482..23456
                     /gene="cysB"
                     /locus_tag="K0W59_002198"
     CDS             22482..23456
                     /gene="cysB"
                     /locus_tag="K0W59_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HBI3023221.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            23751..23915
                     /locus_tag="K0W59_002199"
     CDS             23751..23915
                     /locus_tag="K0W59_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HBI3023222.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            23918..24085
                     /locus_tag="K0W59_002200"
     CDS             23918..24085
                     /locus_tag="K0W59_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023223.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            24199..24294
                     /locus_tag="K0W59_002201"
     CDS             24199..24294
                     /locus_tag="K0W59_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023224.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            24458..27133
                     /gene="acnA"
                     /locus_tag="K0W59_002202"
     CDS             24458..27133
                     /gene="acnA"
                     /locus_tag="K0W59_002202"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HBI3023225.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTQYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(27197..27787)
                     /gene="ribA"
                     /locus_tag="K0W59_002203"
     CDS             complement(27197..27787)
                     /gene="ribA"
                     /locus_tag="K0W59_002203"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HBI3023226.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            27957..28721
                     /gene="pgpB"
                     /locus_tag="K0W59_002204"
     CDS             27957..28721
                     /gene="pgpB"
                     /locus_tag="K0W59_002204"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HBI3023227.1"
                     /translation="MRSIARRTAVGAALLFVMPVAVWISGWRWQPGEQSWRLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFSILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            28870..29178
                     /gene="lapA"
                     /locus_tag="K0W59_002205"
     CDS             28870..29178
                     /gene="lapA"
                     /locus_tag="K0W59_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HBI3023228.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            29185..30354
                     /gene="lapB"
                     /locus_tag="K0W59_002206"
     CDS             29185..30354
                     /gene="lapB"
                     /locus_tag="K0W59_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HBI3023229.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAVADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            30547..31284
                     /gene="pyrF"
                     /locus_tag="K0W59_002207"
     CDS             30547..31284
                     /gene="pyrF"
                     /locus_tag="K0W59_002207"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBI3023230.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            31284..31610
                     /gene="yciH"
                     /locus_tag="K0W59_002208"
     CDS             31284..31610
                     /gene="yciH"
                     /locus_tag="K0W59_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HBI3023231.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
BASE COUNT         7943 a         7699 c         8136 g         7911 t
ORIGIN      
        1 tcacagcctc agctaatgag cgagcttcaa aagtgcttaa gtcgggtaat gctttacgga
       61 agtactctgt gaatccgaat aagaagggcc ttattgagac ataaaaaggg gcttcatagc
      121 ccctgaaaat tacagttgat aagcatcaat atccagtagc tctttatcag caatttcaaa
      181 gattggtgac ccatcattta gcggtagcgg gtctgtaggt atggtgattt cagtgtcgtt
      241 ctgtagcgtt atagttactg aacccttacc caatgtaagc ccttttagat gtttggacaa
      301 aattaattga cactcgacac gacccgcttt agttgtcagg tcaatatctt taagctgggg
      361 tagttcctta tcagtgagtg aagccttacg cttcatagta tcaatcgtgg tctgtagttc
      421 tgtgcgcttc tggttaagtt ccttaagctg aatttttagt tcatcaatat catccatctc
      481 aagaagtagg gcggtagctt tcttgattct ggctgtgaca atttcaacct catgctgtaa
      541 cgctgcaatc ttcctctctg attctccatc attccccacg tcaatcatcc ccagaaaatt
      601 atcaatcatc catcggtcaa acggtttacg cttcaacggt ggaacatcac agcgaccgtc
      661 taagtggtta ttgcacctgt agaccccagc atacttactc tttgttccgg taggatggac
      721 actagcccca cactgacacc taaacagacc tttgaagatg ttaatcgtta gtgggttgtc
      781 acttgcaggt gtacggcctt tacgattctt atcaagaatc tcttgagctt tattgaacgt
      841 tgcagcatct atagcggctt cgtagtagtt cggtatagcg gggcggtcat tgcgctgtga
      901 tgctggtagt gtacctatcg ttgtctctga ccttaagata gtacgcacag aatgaacgct
      961 aaatccatta gcatctttac gtcgtgagaa tgaactaatg ttattagcct gtaggtgacg
     1021 tgcaatgtta tgcagacttt caccatctag cagcagcttg aaaatcaagc gtacagtttc
     1081 agcatgttca ttgagtttaa aagtctcagg gtcaatccat ccgggaacat tccttgttaa
     1141 acgttgttca ccagcaaggg caagctttgc acgttgttcc catttctgac taacacggaa
     1201 agacttctgt ttactctcct gatgcgctat gtagctttta acccgctgaa tgattgagat
     1261 tcctaaatca tcggatagag catcccttgt gtagattagc ccatcctgta gagtgtgaat
     1321 ctcgatacca tgagcaataa tgcgattcat caaggcttct gtttcaagat gttcaaggcg
     1381 gcctatgcgg tcgatagctt cgactagtaa aattgtccct tctggaaact cccctgattc
     1441 aatgcgggag agcatatccc caaattgccc tgataagtgt ttacctttgt aaccggatag
     1501 accttcatca ttgaaagatg ccactacctg agcatcgcta ttcttcctta accactgttt
     1561 gaatagctca ttttgtcgtc tggttgaatc agcaccttca gcaccttgtg tggctgatga
     1621 gaatctcaag tatgttatta cggttctcac ggtgataaac tcccatgcaa ttgattatca
     1681 tttaaagatg aacttataaa cgttatggct aatgctacag tgccgaatag ctacaataca
     1741 atgccccttg atttacctcg ccgcttcccc tggccgacgt tactttcggt ctgcattcat
     1801 ggtgctgttg tggcgggtct gctctatacc tcggtacatc aggttattga actacctgcg
     1861 cctgcgcaac caatttctgt cacgatggtt gcgcctgctg atctcgaacc gccacaagcc
     1921 gttcagccgc caccggagcc ggtggtagag ccagaaccgg aacctgagcc gatccccgaa
     1981 ccgccaaaag aagcaccggt ggtcattgaa aagccgaagc cgaaacctaa gccaaaaccg
     2041 aagccaaaac cgaagccggt gaaaaaggta caggagcagc caaaacgcga tgtcaaaccc
     2101 gtagagtcgc gtccggcatc accgtttgaa aatacggcac ccgcgcgccc cacatcaagt
     2161 acggcaacgg ctgcaaccag caagccggtt accagtgtgg cttcaggacc acgcgcatta
     2221 agccgtaatc agccgcagta tccggcacga gcacaggcat tgcgcattga agggcaggtt
     2281 aaagttaaat ttgacgtcac gccggatggt cgcgtggata acgtacaaat cctctcagcc
     2341 aagcctgcga acatgtttga gcgtgaggtg aaaaatgcga tgcgcagatg gcgttatgag
     2401 ccgggtaagc caggcagtgg gattgtggtg aatatcctgt ttaaaattaa cggcaccacc
     2461 gaaattcagt aagcagaaag tcaaaagcct ccgaccggag gcttttgact attactcaac
     2521 aggtaaggcg cgaggttttc cttcaggatc aaccgcgaca tacttaaata atgcttctgt
     2581 cgctttatag cgttgcccga ttggttcaga cgctactttt ttcacccaca cttcaatatt
     2641 aatgctgacc gatgtcgtcc ctttctggac acagcgtgca tagcagcaca ccacatcgcc
     2701 gaccgcaacc ggccgtaaga aagtcattcc ttcaacccgc acagtcacta cgcgaccgtg
     2761 ggcaatttct tttgccagaa tagcgccgcc aatatccatt tgtgacatta accaaccacc
     2821 aaagatgtca ccattggcat tggtatcggc gggcatggct aaagtacgta aaacaagatc
     2881 gccctgaggg acgttatgtg ttgtagacat ggtaaaaccg acttaaaagg aaaatcacag
     2941 gcgcgatgct actatgattt tcctgaggag aacagaggag atacagcgcc agccccgaag
     3001 gactggcagt ctggttagga tttatcttcc tgcggcatgt ggcggtagat atagataccg
     3061 cttaacaatg tgaagattaa ggtaagggcg gtcaggccaa agactttaaa gttgacccaa
     3121 atattttgcg gcagccagaa tgcgatgtag atgtttgcca gaccgcaaag gataaagaaa
     3181 acagcccagg ccagatttag cttcgaccat accggttgcg gcagcgtgag ttctttaccc
     3241 agcatccgct gaattagcgg ctttttcatc acccattggc tgactaacag ggctaccgca
     3301 aacagagcat aaatgactgt aaccttccat ttaataaact catcattgtg gaagaacagc
     3361 gtcaagccac cgaagacgac caccagaaca aaagtgatca gggccatctt ctcaacctta
     3421 cgaaagcgaa cccagctata gataagcaca atcgccgtgg cgacgatcag cgctgcagta
     3481 gccgcataga tgtcataaat cttgtaaaac gcgaaaaaga caaccagcgg taaaaaatca
     3541 agaaactgct tcattttacg attccgtaat caaatgctta aggttattgg cgaatcaaca
     3601 tatacaggcg gaacagatag atgagcaata tggcagaaat caggttgctc aaggtgttcg
     3661 ccagtacggc accaatttcc ggggttaatg cggcaaaaga agaggcaaaa agcagcaaca
     3721 gtgtttttgc cagcaaccag ctcagtactg cgggtgccac cagacgcata ttcgcccaag
     3781 tcagccgcat actgctacgc atcgaggcaa aaatgcccat tttgtcctgt accagcatca
     3841 ccggtgccag ggccagtaaa atggccatga taattcccgg aacgaccacc agcatgatgc
     3901 caatctgtac taaaagggta gtcagaaaaa tcagaataaa taacttgggc aatatcggcg
     3961 cactggcacc aatagctcgc agcgcacaga ctctctgacc cgcagacacc agctggataa
     4021 ttaatattac gcctccggcg agaatggcgt taccgattaa ttctgaaaaa gtggacgccg
     4081 ctgaagcctg cagcaaaatt tgttgctgtt ccggtgacat attctgaacc aggtcgaaca
     4141 acccactact gccgctaacg ggcacgccgt cattgagctg cgcaagctgt gcatcactgg
     4201 gtgagaaaac atgccctaac accactgtga taaacgcaca tagcaacgat accaacagaa
     4261 tggtcataaa ttgattacgg aagaaatttc cggtgtcacg gtatacggac tgcgccgtga
     4321 tagacatgca ctctccttga gttttatgca ggtgttaatt agcgggcaat tgtaccctgg
     4381 ttaagccttg ggtggcagca tcaggtcttg tatggaaaag catatctttg taaagcggag
     4441 gtaattgctg gccttcaggc gtgggcaaac cgcggcaagt ggcgatcgag ttaatatttg
     4501 cgtagagaaa atatttaaaa attgatttaa atcacattaa ccaggattct caatgcaact
     4561 tctaaattaa tccagatcaa taaatggtga attatcatat gtaatgtgat ctatgtagga
     4621 tcatttgtta ctccaatgta ggtatattcg tcacgttttt ataaccataa cgacggagcg
     4681 gatatgaaaa agttaacagt ggcggctttg gcagtaacaa ctcttctttc tggcagtgcc
     4741 tttgcgcatg aagcaggcga attttttatg cgtgcaggtt ctgcaaccgt acgtccaaca
     4801 gaaggtgctg gtagtacgtt aggaagtctg ggtggattca gcgtgaccaa taacactcaa
     4861 ctgggcttga cgtttactta tatggcgacc gacaacattg gtgtggaatt actggcagcg
     4921 acgccgttcc gccataaaat cggcacccgg gcgaccggcg atattgcaac cgttcaccat
     4981 ctgccgccaa cactgatggc gcagtggtat tttggtgatg ccagcagcaa attccgtcct
     5041 tacgttgggg ccggtattaa ctacaccacc ttctttgata atggatttaa cgatcatggc
     5101 aaagaggcgg ggctttccga tctcagtctg aaagattcct ggggagctgc cgggcaggtg
     5161 ggggttgatt atctgattaa ccgtgactgg ttggttaaca tgtcagtgtg gtacatggat
     5221 atcgatacca ccgccaatta taagctaggc ggtgcacagc aacacgatag cgtacgcctc
     5281 gatccgtggg tgtttatgtt ctcagcagga tatcgttttt aattccgcac aaaaacgacc
     5341 ccgtaatata cggggtcaat aaggacatgg tataaagggt attatttctt cgcttctacg
     5401 ccatcagttt cagaacgaat taaaaatttc tcagttgttt gcggaatatg ctgaatcagc
     5461 cagtcggcca tttgcttttc ctcatttaaa atcgcttcga tgatcggaat tgagactgta
     5521 tcaccggcat tttttgctgc tgctaatagt gaggtgtaac aggcgatttc aaattgctcg
     5581 aagacatatc cgctaataga gcctttaact atttcatcag aagggaatat accaccgatt
     5641 gactgcccaa gcgcagccat tttactcatg gaatctttaa tgactgaacg tgaaatgtca
     5701 ttacgatcaa gaatagtttc cagttgaaca atctggtttt tggtttcact aagatgttgt
     5761 tcaatacgag cgcgtagttc aggataatta tctatacggc tggccatgga ttcaagcata
     5821 gattcggctt gcttttccat tgcgtgggcg tcacgtaacc agtcatgata atgttcaata
     5881 cgattcatga aaatgtcctc tttataaaat gactaaaagt taaaattcat atttcaggct
     5941 ttattttcgg ctttcttatt tacgttatta agggccagat cagtcagttt gatgtcggtg
     6001 gccttttctt cttccagggt ttctttcaga agcttcgctg ctttacggta acctaattgt
     6061 tcagccagcg tcgctaatgt cccgtaactg gcaatctcat aatgctcgac tttctgtgct
     6121 gcggcaatca gtgcggcatc acgcacttcg tttttctcgg tactttcgat gacctcatta
     6181 gcttcttcaa taagaccttc cattgccaca catttcatgc gcttaatttt cagatttgat
     6241 tctgattcca caacttgatc aattcgttca atctgtccat gagtttcctc gaggtgcgca
     6301 tgaaaagcct gacttaattt ttcatttgat gttgctcttg cgagttttgc cagtgcccgg
     6361 ggtaattgtt tttctgcgct gtaggtatct gaaagcagtt gaataaatac atcttcaatg
     6421 gtcttcatat tcatattttt ctccagtgaa atcactgcga gccatacggc ccgcagatgc
     6481 aattatcttt atcagctgat attaataatt aacggtgaat caggatttgt ctgatttatt
     6541 accaccgctt tgttgaccgc cttttttacc cgcttcagat gcgcgttgcg gatcattttt
     6601 aaaattaccg ccgctatgct gaccgccttt acggcctgcg tcggatgcct tctcacggtc
     6661 ttcggcgaaa tttcctgaac caccacgatg ttcggccatg ttatttctcc cgttgcgttg
     6721 cattgtttca ttaatatgag tgttgtgtgt cgacactcat taaaattagt cgctaatgag
     6781 aattagtcaa attaagcgca acgagaagat agagggaaaa tatattttga ggaacattct
     6841 ggatatatta acaattacct gaggaataaa tgacttagag aaaattattg attttactgg
     6901 tgttatgttg cgggattaat ttgtttgatt atattactgt tgggcggaaa aatgacgtaa
     6961 gttgacgttc gaccggggta agcgaaacgg taaaaagata aatattaaat gaatttagga
     7021 tttttccggc ttcattaaag aaagttaaaa tgccgccagc ggaactggcg gctgtgggat
     7081 taactgcgcg tcgccgcttt catcggttgt acaaaaactt tcagtgccgc cagcattttc
     7141 tctggctcat taatatgttg ctcgatgatt ttaacaatgg ccgaaccaga aatcgcgccc
     7201 gcagctcctg catcaatcgc tgcttttacc tgatccgggg cggaaatacc aaacccctgc
     7261 aatggaggtg cagcgttgta ctctttcagc ttcgcaacca gatgattgag gggtaacgcg
     7321 gcgcggtttt ctgcgccggt cacgcccgct cgtgacagca aatatgtgta accacgaccg
     7381 taagaggcta tctggcgcag caggtcgtca tcggcatttg gcgggcagat gaagataggt
     7441 gcgacattat ggcgcaacgc ggcctggcgg aagggcgcgg actcttcaac tggcacatcg
     7501 gcaaccagca ccgaatcgac gccgactttc tcgcactggg cataaaactc atcaatgcct
     7561 ttgttaaaca ccagattggc atacatcaac aggccaatgg gaatggtcgg gtgtttctgg
     7621 cgaatcagtg ccagcatttc aaaacattgt gccggagtca cacctgccgc aaaggcgcgc
     7681 agagtggcgt tttgaatggt cgggccatcc gccagtgggt cggagaaggg gatacctaac
     7741 tccagcgcgt cagcaccggc ttcaattagc gtatcgataa ttttcaatga ctgctcaatg
     7801 cccggatcac cgagcgtgac gaaaggaacg aatgcgcctt ctttgcgctc cttcaactgg
     7861 gcaaacagag attcgtagcg ttccatcaga tttcccctcg tgctttcaaa atatcgtgaa
     7921 cggtgaagat gtctttatcg ccgcgaccgg aaaggttaac caccagtagc tgctctttat
     7981 ccgggttttc gcgcatcatt ttcagcgcat gggccagggc gtgggaggat tccagcgccg
     8041 ggatgatccc ttcgtgcagg cacagcgttt tgaaggcttc aagggcttca tcatcggtaa
     8101 tagacacgta atcagcgcgt ccagtgctgt taagatacgc gtgttgtggg ccgacagacg
     8161 ggaaatccag tccggcggag atggagtaag attcttcaat ctgcccgtct tcggtttgca
     8221 tcatcggcgc tttcataccg aaataaatgc ccacgcgacc atgttttagc ggtgcgccgt
     8281 gctcgccagt ttcgataccg tgaccaccag gctccacacc aatcaggccg acgttggttt
     8341 cattgatgaa atcagcaaac atgccgatgg cattcgaacc gccgccaaca caggcgataa
     8401 cggcatccgg caggcgacct tctctttcca gaatctgcgc tttggtttct tcgccaatca
     8461 tccgctgaaa ctcacggaca atggtcggat aaggatgcgg gccagccgcg gtgcccagca
     8521 tatagtgcgc ggtttcataa ctgccggacc agtcgcgtag cgcctcatta caggcatctt
     8581 tcagcgtggc ggaaccgcta tgtaccggga tcacttccgc acccattaag cgcatacgaa
     8641 aaacgttagg cgactggcgt tcaacgtctt tggcacccat ataaatacgg catttcaggc
     8701 cgagcagggc gctggcaagg gccgacgcca cgccatgctg accggccccg gtttcggcga
     8761 tgatttcggt tttacccatc cgcttcgcca gcaacgcctg ccccagcacc tggttggttt
     8821 tatgtgcgcc gccgtgcagc aaatcttcac gcttgagata cagcgtggtg ttcgtcccgg
     8881 ctgtaatgtt ctggcatttg gtcagtgcgg ttggacgccc ggcatagttt ttcagcaggt
     8941 cgttgaactg ggcttgaaat tcaggatctt tttgcgcgct gacaaaagct tcttccagct
     9001 ggcgcagagc aggcatcagg atttgtggca cgtacatgcc gccaaactca ccaaaatagg
     9061 ggttaagtaa tgttgtcatt gttcctttcc ttaatatgcg cgcagcgtct ggaaaaccga
     9121 ggccaaaaga cgtgcgtctt tgatgcccgg ttgcgactct acagcagaat taaaatcaag
     9181 tccggcgcag ccggtttgtg ccgcttccac gcagttatct gcgcctaagc cccccgccag
     9241 cagaacgttg ccaagcgatt gaccatttaa tagtgaccag tcaaaacgtt gcccgcttcc
     9301 accctggccg ttgtctaaaa catatttatc aacgtgctga aactcgcggg cgggcagggt
     9361 ttcaccgacg cttaatgctt tccagatggc aacatgtgct ggcagagctt cacgcagcgt
     9421 atcgatatac agctgatctt cattaccatg cagttgcact gccgccagcg ataacacctt
     9481 agctttgtcc accacatcgg caatatcgtg attgcggaac acgccaacat actgcaacgg
     9541 tgccgcagcc atcacttcct gcgcctgttc aacgttgacg caacgcggtg atgtcgcaac
     9601 aaaaatcaac ccaccgtaaa tcgcgcccgc gtcataagct gctttagcat cttgcccacg
     9661 cgtcaggcca catactttat tctcacccag caacacccgg cgcacggcgg cgtgcaaatc
     9721 gtcatgggcc atcaacgccg aaccaatcag aaaaccgtta gcgaagtggc ttaactcgcg
     9781 cacctgagcg taagtattga tgccggattc gctgattacc gttacgttgt gccccagttt
     9841 cggcgcaagc tcgcgggtac ggttgagatc aatcgacaaa tcacgcagat cgcggttgtt
     9901 gatgccaacg acctttgccc ctaatgcaat ggcgcgctcc agttcctctt cattactgac
     9961 ttcggtcagc acccccatct ccagactgtg agcgacggct gcaagctggc gatattggtc
    10021 gtcatccagt actgaaagca ttaataagca ggcatcggcc tggtaatagc gcgccagata
    10081 gatctggtaa gggtcgataa tgaagtcttt acataaaatc ggctgcgggg cgatttggct
    10141 gacgatgagg aggaaatcaa agctcccctg aaaatatttc tcatcagtca gcaccgaaat
    10201 tgccgaagcg taatgtttat aaatggcggc aatgcgtgcc ggatcgaaat catcacggat
    10261 cacgcctttt gacggcgacg cttttttaca ctccagaata aacgccgtgc gtgcaccctg
    10321 tagcgcatca taaaaatgtc gtgtgctcgg ctgaacctca ttctgaaaac tggccagcgg
    10381 ttgctgctgt ttgcgggttt ctacccaaat cgccttgtct gcgacgattt tcgctaaaac
    10441 ggtttgcatc atttaccctc gtgcagccag cgcggtaact ctgtcgtaag cggaaccact
    10501 gcgcagtacc tcaagaacgg tttgcgcatt ggcttgcaga tcttcatggc catgcaggcg
    10561 cattaacatg gcgacgttcg cagcgacggc tgcttcatgg gcggcgtcgc ctttaccttg
    10621 taacaagcgt gttaaaatgt cacggttttc ttcaggtgtt ccgcctgcca gttgctcctg
    10681 gtggtaggga gtcaggccaa aatcttcagc ggtcaattga tagctcttaa ttttgccgtc
    10741 atgcagctcg gcaacgattg tcggcgcgtg taatgaaact tcatccatcc cgccgctgtg
    10801 caccaccgcc gcgcgttgat accccagcac acgcaaggtt tcggcaatcg gcagcaccag
    10861 ttccggacta taaacaccaa ttaacgccag cggcggatgc gccgggttaa tcaatggccc
    10921 cagcacattg aacagggtgc gggttttcag ttgctggcga accggcatcg cgtggcggaa
    10981 tccggtgtga tatttcggcg caaagaggaa acatacgcct aactcgtcca gcgcctggcg
    11041 cgatttattg gcgttcatat caagattaat accgaatgcc gccagcagat ccgacgagcc
    11101 ggatttactg gagacgctgc ggttaccgtg tttcgccact ttcagcccac aggccgcggc
    11161 gacaaacgca ctggcggtag aaatattgat actgttgctg ccgtcaccgc cagtaccgac
    11221 gatatcagca aacagataat ccgggcgcgg gaacggcgct gcgttttcca gtagcgcggt
    11281 tgctgccccg gcgatctcgt tcgggtgctc accgcgaatt ttcatgctca ccagcgccgc
    11341 cgccagttgt tccggcttca gctcgccacg caccaccgct gaaaacagct ggtggctttc
    11401 ttgttggcta agcgtctgcg cctgatacag tttttccaga atcggttgca gcgtgttggc
    11461 tggctctagt ttctgctgcg cccaggccag cgtttgttcc agcaggcgag cgccctgggt
    11521 ggtgagaatg gattccggat ggaactggaa tccacaaacg cgatccgcat cgtgacgtac
    11581 tgccatcacc atgccattaa aatgggcgtt gatggttaaa ccggccggaa tgttactgcc
    11641 aaccagcgag tgataacgcg ccaccggcag cgggtttgtt aatccggcaa acatcgcctg
    11701 accgtcatgt tcaatgctcg acgctttacc gtgaagaatt tcgcccgcct gaccgacata
    11761 gcccccgtaa gcttcgacaa ttgcctgatg tccgaggcaa atgccaataa tgggcagctt
    11821 gccacgcaag cgggtgagga gttccggcat acaaccggct tcgctcggca caccggggcc
    11881 aggagaaagc atcagtaccg gattgctcat cgtcgccagg cgttcaatta aggtctgcgc
    11941 cggaatatgg ttgcggtaaa tcaccacgtt atgaccattg ctgcgcaact gatctgccag
    12001 gttgtacgta aaagagtcga tattatcgag cagcagaatg tcagccatca gaaagtctcc
    12061 tgtgcatgat gcgcggtggc aatagcgcgc agtacagcgc gggctttgtt acgggtttcg
    12121 tcggcttccg actgcggaac agaatcaagg actacaccag cacccgcttg cacggtggcg
    12181 ataccgtttt ccaccagcgc cgagcggatc acaatgcagg tgtcgagatc gccatgcgcg
    12241 gtgaaataac ctaccgcgcc gccgtagctg ccgcggcgac gaccttccgc ctcggcaatt
    12301 aactgcatag cgcgtacttt cggcgcaccg cttaacgtcc ccatattcat acaggcgcga
    12361 taagcgtgca gggcgtcaag atcgtgacgc agttcgccga ctacgcgaga gacgaggtgc
    12421 atcacatagg aataacggtc aactttggtg agatcggcga cgtagcggct gccaggggtg
    12481 caaatgcgtg ccagatcatt acgggcgaga tcaaccagca tcagatgttc agacagctct
    12541 ttatggtcag tacgcatttc cagttcaata cggctgtcga gatctctgtc cagtgaacca
    12601 tcggcgcgac gaccgcgtgg gcgtgttccg gcaatcgggt agatctcaat ctggcggctg
    12661 gtggcatcat acttgagcga gctttccggc gacgcgccaa atagggtgaa atcattatcc
    12721 tgcataaaaa acatgtacgg gctgggatta ctctttttca gcacgtaata ggccgccagc
    12781 ggtgacgggc agggcagaga gaaacggcga gatggcacca cctggaaaat ttctccagcg
    12841 cgaatcgctt tttgcaacaa acgcactacg ccaccgaact cttcatcgct ctgattacat
    12901 tcacaacgca tatgcggcac ggaaaccact ggcagcggcg gcgcggcttc ggtcagttgc
    12961 tgacgtagtt cgttcaggcg agcagtaaga cgttgttttt cttcttcatt cggagcaaac
    13021 aggctggcct gaatacgggt gctttttttc tgatggtcaa tcaccatcag cgtttcagcg
    13081 agataaaaac agaaatcagg gcagttattt tccgctgaca gttgcggtaa atcttcaaat
    13141 cccgccacaa ggtcataaga gaacaggccg ccgaagaaca ttgcttctcg ttcttccttc
    13201 ggtacattca acagattctg caataaacgg aaagcgtcaa aaaccgaaag ggagcataag
    13261 cgggcgtctt catccagcag tggactgaca ggggggaagc gcagcacacg gcagtttggt
    13321 gattgttcac tttccacacc cgcaggcagg gcgttatcca gtagtgccag cagggcttcg
    13381 ccattgccgg aaagtgcctg aattgtgacg gtgtcaccta atgctgtaat gtgcagcgca
    13441 ctgtctatca gcagcaggct ttttaaatca tctttgctgt cgatatctgc ggattccagc
    13501 agcagcgttg ccggacgatt cccacacaac tggtgaaaaa gcgcagtcgg gttgtcgcga
    13561 taagcgcctt cgcaggttag cagttcgaga gtcggttttt gtgtttgcat tgttattctc
    13621 taattttgtt caaaaaaaag cccgctcatt aggcgggctg ggtatctgat tgctttacgc
    13681 atggtgaata cactgcccgt ttcaggaagt ggccaccaac ctttcagtac gaaaattgct
    13741 ttcattgtcg ataccatttt tacgtgaact tgcgtactag tcaactggtt cgatgattaa
    13801 ttgtcaacag ctcatttcag aatatttgcc agacccgtta tgatgtcggc gcaaaaaaca
    13861 ttatccagaa cgggagtgcg ccttgagcga cacgaattat gcagtgattt acgacctgca
    13921 cagccatacc acagcttccg atggctgcct gacgccagaa gcattggtgc accgtgcagt
    13981 cgagatgcgc gtcggcaccc tggcgatcac cgaccatgac accacagctg caatcgcgcc
    14041 agcaagagag gaaatttcac gttcgggact ggcgctgaat cttattcccg gcgtggaaat
    14101 ttccacggtc tgggaaaatc atgaaattca tattgtcggg ctgaatattg atattactca
    14161 tccgctaatg tgtgagttcc tcgcgcagca gacagaacgg cgcaatcagc gggcgcagca
    14221 aattgccgaa agacttgaaa aagcgcaaat acctggggcg ctggaaggcg cacaacgact
    14281 ggcgcagggc ggcgcagtga cgcgcggtca ttttgcgcgt ttccttgtgg agtgcggcaa
    14341 agccagttca atggcagatg tctttaaaaa gtatctggcg cgcgggaaaa ccggatacgt
    14401 tccgccacag tggtgtacaa tagaacaagc tattgatgtc attcatcatt ctggcggtaa
    14461 ggcggtgcta gctcatcctg ggcggtacaa tcttagtgct aaatggctga aaagattggt
    14521 tgcgcatttt gccgaacacc acggcgacgc gatggaagtc gcgcagtgcc agcaatcgcc
    14581 caatgaacgc acccagctgg ccacacttgc gcgtcagcat catttatggg catcacaagg
    14641 atctgatttt catcagccat gcccgtggat cgaactgggt cgtaaactct ggctgcccgc
    14701 aggcgttgaa ggcgtctggc agctatggga acagccgcag aacaccacag agagggaatt
    14761 atgagccagt ttttttatat tcatcctgat aacccgcagc aacgtctgat caaccaggcg
    14821 gtggagatcg tgcgtaaagg cggggtgatt gtctatccaa ctgattccgg ctatgcgctc
    14881 ggctgtaaaa gtgaagataa aaacgccatg gagcgtattt gtcgtattcg ccagctgccg
    14941 gacggtcaca actttaccct gatgtgtcgc gatctttctg aactctcgac ctattcattc
    15001 gtggataacg ttgcgtttcg tttgatgaaa aacaacacgc cgggcaacta taccttcatc
    15061 ctgaagggga cgaaggaagt gccacgccgt ctgttgcagg agaaacgcaa aaccatcggt
    15121 atgcgtgtgc cttctaaccc gattgcgcag gcgttgcttg aagcactggg cgaaccgatg
    15181 ctttccactt cgctaatgct gccaggcagc gaatttaccg aatccgatcc cgaagaaatt
    15241 aaagaccgtc tggaaaaaca ggtagatttg attattcacg gcggctatct cgggcagaaa
    15301 ccaacaacgg ttattgatct taccgatgat acgccggtcg tggtgcgtga aggcgtaggt
    15361 gatgtgaagc ctttcttata agtgcgtaac tctgtatact acgcggcctt aaaatggcgc
    15421 tatacggcgc cattgattct cctgttcgac gcctgtgaag gcgacaccaa aaggaagctc
    15481 tatgagcgaa aaattacaga aagtgctggc ccgcgctggc cacggctccc gccgtgaaat
    15541 cgaatctatt attgaagccg gtcgtgtgag tgttgatggc aaaattgcta aactcggcga
    15601 tcgcgttgaa gttacccctg ggctgaaaat tcgtatcgat ggtcatctga tttcggtacg
    15661 tgagtccgct gaacaaattt gtcgcgttct ggcctattac aaaccggaag gtgagttgtg
    15721 tacccgtaac gatccggaag ggcgtccgac ggtgtttgac cgtctgccaa agctgcgtgg
    15781 cgcacgctgg attgccgtag gtcgtctgga cgttaacacc tgtggtctgc tgctgttcac
    15841 caccgatggt gaactggcaa accgtttaat gcatccaagc cgtgaagttg aacgtgaata
    15901 cgccgtgcgt gtatttggtc aggttgacga cgcgaaactg cgtgatttga gtcgtggcgt
    15961 gcaactggaa gatggtccgg cagcttttaa aaccatcaag ttcagcggcg gcgaagggat
    16021 caaccagtgg tacaacgtga ctctgaccga aggccgtaac cgtgaagttc gtcgtctgtg
    16081 ggaagcggtt ggtgtgcagg tgagccgcct gatccgtgtt cgttacggtg atatcccact
    16141 gccaaaaggt ctgccacgcg gtggctggac ggagctggac ctcgcccaga ctaactatct
    16201 gcgcgaactg gtggagcttc cgccagaaac cagctctaaa gtcgctgtag aaaaagaccg
    16261 tcgtcgcatg aaggctaatc agattcgtcg tgcggtgaaa cgtcacagtc aggtgagcgg
    16321 cggtcgtcgt tctggcgggc gtaataacgg ttaatcaaag tatgccggac gtcatatccg
    16381 gcatttttac agattaataa tcaatcccta tttgcgcctt aacaccggca tcaaacgcat
    16441 gtctgatggg gcgcagctca cttaccgtat ccgccagttc aagaatatcc cgatgacagc
    16501 ctcgacccgt gataatcacc gtctgttgat gtggacgttc atttaacgcc tgcaccactt
    16561 cttccagtgg caaatagtca tacgccacca tatacgtcag ttcatcaagc aaaaccatat
    16621 ccagtgagga atcagcaagc atccgctttg catgttgcca gacttcgcgg caggcggcgg
    16681 tatcagactc gcggttttgt gtatcccagg taaagcccgt tgccatcacc tgaaactcaa
    16741 cgccatgtgg ctccagcaga ttgcgttcgc cattaggcca ggtgccttta ataaactgca
    16801 cgacgcccac tttttttccg tgaccaactg cgcgtgttgc cgtaccaaat gccgcggtgg
    16861 ttttgccttt tccattgccg gtaaagacga tgataatacc gcgttcatcc tgggcctggg
    16921 ccacacgagc atctactttt tctttcactc gctgctgacg ctgttggtag cgttcatcac
    16981 tcattgggaa attcctggtt tacggcccgg ttgggcgtca aaggtcatgc cggttttacg
    17041 gcggctgtca tcgcccatca gccagaggta gagcggcatg atatcagcgg gtgttttaag
    17101 tttctgtgga tcttcggtcg ggaaggcgct tgcgcgcatt gcggtgcgcg taccgcctgg
    17161 gttaatgcag ttgacgcgca ggcgctgctg atattcatcg gccagtacct gcatcatccc
    17221 ttcggtggca aatttcgacg ctgcatatgc accccagttg gctcgtccct gacgtccaac
    17281 gcttgatgaa gtaaagacca gtgaaccggc gtccgatttg agtaataaag gaagcagtgc
    17341 ctgggtgagc ataaaggtgg cattaacgtt gacctgcatg acgtcctgcc agacctgcgg
    17401 attttgttcg ctcattgggc aaacatcgcc gagcaatccg gcattatgca aaacaccatc
    17461 cagacgcgga taattaacga caatgcgctg tgccagttgt tggcaatttt cggacgtgca
    17521 ggtcagcaaa tcgagaataa accactgtgg ctgacgccca gtttcttcgt ttatgtggct
    17581 ggctacctga cgtaattttt cttcattacg gcccaacaga atcactgtcg caccatagcg
    17641 tgcatacgtc atcgcggctt cacgaccaat cccatcgctg gctcccgtca ccaggataat
    17701 gcgatcattg agtaaatctt gttttggctg gtaatgcatg gctactcctc aacgacgttg
    17761 tctgtcgcgc tgccagtgta ctttatgact ttggggcttt atgcctgaaa cagaagggca
    17821 tttcaatcag cctggggcga taacaccgca aaatggatac tttgtcatac tttcgctgca
    17881 ataacatctc tgcgagacgg cttaacatgc ctgttgtaaa ctgtgagcca aagcgttgtt
    17941 taaccaaggt ggggactcgt ggaattgttg tctgaatatg gtttgttttt ggctaaaatc
    18001 gttaccgttg tgctagcgat tgcggcgatt gccgccatta ttgtcaatgt tgctcaacgt
    18061 aataaacgcc agcgtggcga gttacgggtc aacaatctca gcgaacagta taaggagatg
    18121 aaagaagaac tggccgcggc gctgatggac tcacatcagc aaaaacagtg gcacaaagcg
    18181 cagaagaaaa agcacaagca agaagcgaaa gcagcaaaag cgaaagccaa actgggcgag
    18241 gtggcaactg acagtaaacc tcgcgcctgg gtgctggatt ttaaaggcag catggacgcc
    18301 catgaagtga actcgctacg tgaagagata acggcggtac tcgcagcatt caaaccgcag
    18361 gatcaggttg tgctccgtct ggaaagccct ggtggcatgg tgcatggtta cgggttggcg
    18421 gcttcgcagc tgcagcgtct gcgtgataaa aacattcctt taactgttac ggtagacaaa
    18481 gtcgctgcca gcggcggtta catgatggcc tgtgtggcgg acaaaattgt ttccgcaccg
    18541 tttgctattg tgggttccat tggggtggtg gcgcaaatgc ccaactttaa ccgcttcctg
    18601 aaaagcaaag atattgatat cgaactgcac accgccgggc agtataagcg cacgctgacc
    18661 ttgctgggtg aaaataccga agaagggcgg gagaaattcc gcgaagagct gaacgaaacg
    18721 catcagttat ttaaagattt tgtgaagcgt atgcgtccgt ctctggatat tgaacaggtg
    18781 gcaacgggtg aacactggta cggacaacag gcggtagaga aaggcctggt tgatgaaatc
    18841 aacaccagtg atgaagttat tcttagcctg atggaaggcc gtgaagtggt taatgtacgc
    18901 tatatgcagc gtaaacgact cattgaccga ttcaccggca gcgcggcaga gagcgccgat
    18961 cgattgttgc tacgctggtg gcagcggggt caaaagccat tgatgtaaaa gacaaacgcg
    19021 aggctaagac ctcgcgtttt gctttaatca accagatgat atttttctga aagcacatgg
    19081 gccaggtgtt tgaacatatt aaacaccgcg gtgcttttgg ctgttggcaa tccttgttca
    19141 tctaaaaagt agtcgccggt aaataccagc acgccattac gctgcgtgac gccggtggct
    19201 tcaatccctg cgagcgtatc ctcatgctca cgaatgattt tgttagcctc tttcaacagc
    19261 gtttcgcggt cgatgggttg tgtctctttg ttcattattt actccttaaa caaggacatt
    19321 agtctacgcc aggcatggct tgcagacaaa tataccacgc tggtggcaag agcgccttac
    19381 tggcaacttt ggattttgca tgctaataaa gttgcgtatc ggattttatc aggtacagtg
    19441 tgacgctttc gtcaatctgg caatagattt gcttgacatt cgaccaaaat tccgtcgtgc
    19501 tatagcgcct gtaggccaag acctgttaac tcagtcacct gaattttcgt gaacagagtc
    19561 acgacaaggg ggtgatatcc gcagagagcg agtccatatc ggtaactcgt tgccagtgga
    19621 aggtttatca acgtgcgacg cattcctgga agaatcaaat taggtaaggt gaatatgggt
    19681 aaagctcttg tcatcgttga gtccccggca aaagccaaaa cgatcaacaa gtatctgggt
    19741 agtgactacg tggtgaaatc cagcgtcggt cacatccgcg atttgccgac cagtggctca
    19801 gctgccaaaa agagtgccga ctctacctcc accaagacgg ctaaaaagcc taaaaaggat
    19861 gaacgtggcg ctctcgtcaa ccgtatgggg gttgacccgt ggcacaattg ggaggcgcat
    19921 tatgaagtgt tgcctggtaa agagaaggtc gtctctgaac tgaaacaact ggctgaaaaa
    19981 gccgaccaca tctatctcgc aaccgacctt gaccgcgaag gggaagccat tgcatggcac
    20041 ctgcgggaag tgattggtgg tgatgatgcg cgctatagcc gagtggtgtt taacgaaatt
    20101 actaaaaacg cgatccgcca ggcatttaac aaaccgggtg agctgaatat tgatcgtgtt
    20161 aatgcccagc aggcgcgtcg ctttatggac cgcgtggtgg ggtatatggt ttcgccgctg
    20221 ctgtggaaaa agatcgctcg tggtctgtct gccggtcgtg tgcagtcggt ggcggtccgc
    20281 ctggtggtag agcgtgagcg tgaaattaaa gcgttcgtac cggaagagtt ctgggaagtc
    20341 gatgccagca cgactacgcc atctggtgaa gcgttggcgt tgcaggtgac tcatcagaat
    20401 gacaaaccgt tccgtccggt caacaaagaa caaactcagg ctgcggtaag tctgctggaa
    20461 aaagcgcgct acagcgtgct ggaacgtgaa gacaaaccga caaccagtaa acctggcgct
    20521 ccttttatta cctctacgct gcaacaagct gccagcaccc gtcttggttt tggcgtgaaa
    20581 aaaaccatga tgatggcgca gcgtttgtat gaagcaggct atatcactta catgcgtacc
    20641 gactccacta acctgagtca ggacgcggta aatatggttc gcggttatat cagcgataat
    20701 tttggtaaga aatatctgcc ggaaagtccg aatcagtacg ccagcaaaga aaactcacag
    20761 gaagcgcacg aagcgattcg tccttctgac gtcaatgtga tggcggaatc gctgaaggat
    20821 atggaagcag atgcgcagaa actgtaccag ctaatctggc gtcagttcgt tgcctgccag
    20881 atgaccccag cgaaatatga ctccacgacg ctgaccgttg gtgcgggcga tttccgcctg
    20941 aaagcacgcg gtcgtatttt gcgctttgat ggctggacaa aagtgatgcc tgcactgcgt
    21001 aaaggcgatg aagatcgtat cttaccggca gtcgataaag gcgatgctct gacgctcgtt
    21061 gaactaacac cagcccagca ctttaccaag ccgccagccc gtttcagtga agcatcgctg
    21121 gttaaagaac tggaaaaacg tggtatcggt cgtccgtcta cctatgcgtc gatcatttcg
    21181 accattcagg atcgtggcta tgtgcgagta gaaaatcgtc gtttctatgc ggaaaaaatg
    21241 ggcgaaatcg tcatcgatcg ccttgaagaa aatttccgcg agttaatgaa ctacgacttt
    21301 accgcgcaga tggaaaacag cctcgaccag gtggcaaatc acgaagcaga gtggaaagct
    21361 gtactggatc acttcttctc ggatttcacc cagcagttag ataaagctga aaaagatccg
    21421 gaagagggtg gtatgcgccc gaaccagatg gttctgacca gcattgactg cccgacttgt
    21481 ggtcgcaaaa tggggattcg cacagcgagc accggggtat tccttggctg ttctggctat
    21541 gcgctgccgc cgaaagagcg ttgcaaaacc accattaacc tggtgccgga aaacgaagtg
    21601 ctgaacgtgc tggaaggcga agatgctgaa accaacgcgc tgcgcgcaaa acgtcgttgc
    21661 ccgaaatgcg gcacggcgat ggacagctat ctcatcgatc cgaaacgtaa gttgcatgtc
    21721 tgtggtaata acccaacctg cgacggttac gagatcgaag agggcgaatt ccgcattaaa
    21781 ggttatgacg gcccgatcgt tgagtgtgaa aaatgtggct ctgaaatgca cctgaaaatg
    21841 gggcgtttcg gtaaatacat ggcctgcacc aacgaagagt gtaaaaacac acgtaagatt
    21901 ttacgtaacg gcgaagtggc accaccgaaa gaagatccgg tgccattacc tgagctgccg
    21961 tgcgaaaaat cagatgctta tttcgtgctg cgtgacggtg ctgccggtgt gttcctggct
    22021 gccaacactt tcccgaaatc gcgtgaaacg cgtgcgccac tggtggaaga gctttatcgc
    22081 ttccgcgacc gtctgccgga aaaactgcgt tatctggccg atgcgccaca gcaggatccg
    22141 gaaggtaata agaccatggt tcgctttagc cgtaaaacca aacagcaata tgtctcttcg
    22201 gaaaaagacg gaaaggcgac tggctggtca gcattttatg ttgatggcaa atgggttgaa
    22261 ggaaaaaaat aacctttaat tctgtcaggt ttttataaac aaagggtcgc gaaagcggcc
    22321 cttttttatt gcatattatt ttttcttcac acctatacac taaggctata aatgatatag
    22381 tggttatagt tagcaccttt tttattatta aatcgtatta gtcacccgcc aggtgtgacg
    22441 aaaaaacgat gttctgatgg cgtctaagtg gatggtttaa catgaaatta caacaacttc
    22501 gctatattgt tgaggtggtc aatcataacc tgaatgtctc atcaacagcg gaaggacttt
    22561 acacatcaca acccgggatc agtaaacaag tcagaatgct ggaagacgag ctaggcattc
    22621 aaattttttc ccgaagcggc aagcacctga cgcaggtaac gccagcagga caagaaataa
    22681 ttcgtatcgc tcgcgaagtc ctgtcgaaag tcgatgccat aaaatcggtt gccggtgagc
    22741 acacctggcc ggataaaggc tcgctgtata tcgccaccac gcatacccag gcacgctacg
    22801 cgttaccaaa cgttatcaaa ggctttattg agcgttatcc ccgcgtttct ttgcatatgc
    22861 accagggccc gccgacacaa attgctgatg ccgtctctaa aggcaatgcc gatttcgcta
    22921 tcgccacaga agcgctgcat ctgtatgaag atttagtgat gttaccgtgc taccactgga
    22981 atcgggctat tgtagtcact ccggatcacc cgctggcagg caaaaaagcc attaccattg
    23041 aagaactggc gcaatatccg ttggtgacat ataccttcgg ctttaccgga cgttcagaac
    23101 tggatactgc ctttaaccgc gcagggttaa cgccgcgtat cgttttcacg gcaacggatg
    23161 ctgacgtcat taaaacttac gtccggttag ggctgggggt aggggtcatt gccagcatgg
    23221 cggtggatcc ggtcgccgat cccgaccttg tgcgcgttga tgctcacgat atcttcagcc
    23281 acagtacaac caaaattggt tttcgccgta gtactttttt gcgcagttat atgtatgatt
    23341 tcattcagcg ttttgcaccg catttaacgc gtgatgtcgt tgatgcggct gtcgcattgc
    23401 gctccaatga agaaattgag gtcatgttta aagatataaa actgccggaa aaataatttc
    23461 ttgcgttatt ttcggcacct tttatgtagc gaaggtgccg gaatatattc tcttttgtta
    23521 cttatttcta taccgtgcac aatattttta tccctcatat ttatagggta aattacctgt
    23581 aaagtagtgt ctaattaccc gcctcgcaaa ttccagtttt caaatatcta tttcaggtca
    23641 aaagattgaa ttattcactg gagacgattc gtaaattcgc tggatttttg actaaagttt
    23701 ctttaggatt tgtctcatcg tatgattaat tacaccaatc attcaacgtc ttgctgataa
    23761 gtgatggcga ttatatgagg ttagcaatgc cttctggaaa tcaggaaccc cgccgtgatc
    23821 ctgaattaaa acgtaaagcc tggctggcgg tttttcttgg ttctgcactt ttctgggtgg
    23881 ttgtcgcact gctgatttgg aaagtgtggg gataactatg gtcggtcagg agcaactgga
    23941 gtcgtcacca ttatgccagc atagtgacaa tgaaccagaa acgaaacggg aatgttccgt
    24001 cgttattcca gacgactggc aactaacatc gcagcagcaa gcctttatag aactgtttgc
    24061 tgaagatgat cagccgaaac aataattatc atcattctta ttacccattt ttaatgaatt
    24121 aaagggcttt taatacaccg cagcaataac agcttgagtt atctcaacac aaaataataa
    24181 ccgttaaggg tgtagcctat gatcaacaca aatatgaaat attggtcctg gatgggcgcg
    24241 ttttctctgt cgatgctctt ctgggccgaa ctcctctgga tcattactca ctgatccttg
    24301 accccgctgc ggcggggttg tcatttgctt tgccacaagg tttctcctct tttatcaatt
    24361 tgggttgtta tcaaatcgtt acgcgatgtt tgtgttatct ttaatattca ccctgaagag
    24421 aatcagggct tcgcaaccct gtcattaagg aggagctatg tcgtcaaccc tacgagaagc
    24481 cagtaaggac acgttgcagg ccaaagataa aacttaccac tactacagcc tgccgcttgc
    24541 tgctaaatca ctgggcgata tcacccgtct acccaagtca ctcaaagttt tgctcgaaaa
    24601 cctgctgcgc tggcaggatg gtaactcagt taccgaagag gatatccacg cactggcggg
    24661 atggctgaaa aatgcccatg ctgaccgtga aattgcctac cgcccggcaa gggtgctgat
    24721 gcaggacttt accggcgtac ctgccgttgt tgatctggcg gcaatgcgcg aagcggttaa
    24781 acgcctcggt ggcgatactg caaaggttaa cccgctctca ccggtcgacc tggtcattga
    24841 ccactcggtg accgtcgatc gttttggtga tgatgaggca tttgaagaaa acgtacgcct
    24901 ggaaatggaa cgcaaccacg aacgttatgt gttcctgaaa tggggaaagc aagcgttcag
    24961 tcggtttagc gtcgtgccgc caggcacagg catttgccat caggttaacc tcgaatatct
    25021 cggcaaagca gtgtggagcg aattgcagga cggtgaatgg attgcttatc cggatacact
    25081 cgttggtact gactcgcaca ccactatgat caacggcctt ggcgtgctgg ggtggggcgt
    25141 gggtgggatc gaagcagaag ccgccatgtt aggccagccg gtttccatgc ttatcccgga
    25201 cgtagtgggc tttaaactta ccggaaaatt acgtgaaggt attaccgcca cagacctggt
    25261 tctcactgtt acccaaatgc tgcgcaaaca tggcgtggtg gggaaattcg tcgaatttta
    25321 tggtgatggt ctggattcac taccgttggc ggatcgcgcc accattgcca atatgtcgcc
    25381 agaatatggt gccacctgtg gcttcttccc aatcgatgca gtaaccctcg attacatgcg
    25441 tttaagcggg cgcagcgaag atcaggtcga gttggtcgaa aaatatgcca aagcgcaggg
    25501 catgtggcgt aacccgggcg atgaaccgat ttttaccagt atgttagaac tggatatgaa
    25561 tgacgttgaa gcgagcctgg cagggccaaa acgcccacag gatcgcgttg cactgcccga
    25621 tgtaccaaaa gcatttgccg ccagtaacga actggaagtg aatgccacgc ataaagatcg
    25681 cctgccagtc gattatgtta tgaacggaca tcagtatcag ttacctgatg gcgctgtggt
    25741 cattgctgcg ataacctcgt gcaccaacac ctctaaccca agtgtgctga tggccgcagg
    25801 cttgctggcg aaaaaagccg taactcttgg cctcaagcgg caaccgtggg tcaaagcgtc
    25861 gctggcaccg ggttcgaaag tcgtttctga ttatctggca aaagcgaaac tgacacagta
    25921 tctcgacgaa ctggggttta accttgtggg atacggttgt accacctgta ttggtaactc
    25981 tgggccgctg cccgatccta tcgaaacggc aatcaaaaaa ggcgatttaa ccgtcggtgc
    26041 ggtgctgtcc ggcaaccgta actttgaagg ccgtatccat ccgctggtta aaactaactg
    26101 gctggcctcg ccgccgctgg tggttgccta tgcgctggcg ggaaatatga atatcaacct
    26161 ggcttctgag cctatcggcc atgatcgcaa aggcgatccg gtttatctga aagatatctg
    26221 gccatcggca caagaaattg cccgtgcggt agaacaagtc tccacagaaa tgttccgcaa
    26281 agagtacgca gaagtttttg aaggcacagc agagtggaag gaaattaacg tcacacgatc
    26341 cgatacctac ggttggcagg aggactcaac ctatattcgc ttatcgcctt tctttgatga
    26401 aatgcaggca acaccagcac cagtggaaga tattcacggt gcgcggatcc tcgcaatgct
    26461 gggggattca gtcaccactg accatatctc tccggcgggc agtattaagc ccgacagccc
    26521 agcgggtcga tatctacaag gtcggggtgt tgagcgaaaa gactttaact cctacggttc
    26581 gcggcgtggt aaccatgaag tgatgatgcg cggcaccttc gccaatattc gcatccgtaa
    26641 tgaaatggtg cctggcgttg aaggggggat gacgcggcat ttacctgaca gcgacgtagt
    26701 ctctatttat gatgctgcga tgcgctataa gcaggagcaa acgccgctgg cggtgattgc
    26761 cgggaaagag tatggatcag gctcaagccg tgactgggcg gcaaaaggac ctcgtctgct
    26821 tggaattcgt gtggtgattg ccgaatcgtt tgaacgaatt caccgttcga atttaattgg
    26881 catgggcatc ctgccgctgg aatttccgca aggcgtgacg cgtaaaacgt tagggctaac
    26941 cggggaagag aagattgata ttggcgatct gcaaaatcta caacctggcg cgacggttcc
    27001 ggtgacgctt acgcgcgcgg atggtagcca ggaagtcgta ccctgccgtt gtcgtatcga
    27061 caccgcgacg gagttgacct actaccagaa cgacggcatt ttgcattatg tcattcgtaa
    27121 tatgttgaag taacaagtat ttgcttgccg gttattttgc ttccggcaag caaatgaatt
    27181 acacaatgca agagggttat ttgttcagca aatggcccat tttctcggct ttggtatcga
    27241 gataatgttc gttattgggg ttacgaccta caatcaatgg tacgcgttca acaatattaa
    27301 tccctgcttc ggtcagaatt tcgacttttt tcgggttatt ggttaacaag cggacttcat
    27361 tgacgccaag gagtttgaac atatcagcgc aaagagtgaa gtcgcgctca tcagcggcga
    27421 agcctaactg gtggttagcc tctacggtat cgtaaccttg atcctgcagt gcgtaagcgc
    27481 ggattttatt cagcagacca atgttacgac cttcctgacg gtgatacagc aaaataccac
    27541 ggccttcctc ggcaatttgc gtcaatgccg cttcgagctg gaagccacaa tcgcagcgca
    27601 agctgaacag ggcgtcaccg gtcagacatt cggaatggac gcgcgcaagt accggggtat
    27661 gcccggaaat atcgccatag actagcgcga catgatcgtg tccggttgcc agttcttcaa
    27721 atcccaccat caggaaatcg ccccatgggg ttggcagttt ggcttctgcc acacgtttaa
    27781 gctgcatgaa attctccaga taatgctggt tctgtattgg cttattttgc cataacgaga
    27841 aggggttcac ctaatcacga cgcgtcgatc gttcacggaa tggcacaaat ctgtcaattt
    27901 ttcttggaac tggcattttc agttatgatt gtgggactta tcaaaaagga gaggccatgc
    27961 gttcgattgc ccgacgtacc gcagtgggag ctgcactatt gtttgtcatg ccagtagccg
    28021 tatggatttc tggctggcgt tggcaacctg gagaacaaag ttggcgacta aaagcggctt
    28081 tttgggttac tgaaactgtc acccagccct ggggcgtcat tacacatttg attttgttcg
    28141 gctggtttct ctggtgtctg cgttttcgca ttaaggctgc ctttgtatta ttttccattc
    28201 tggcggcggc aatccttgtt ggacaaggcg ttaaatcctg gatcaaagac aaagtacagg
    28261 aaccacgacc ttttgttatc tggttggaaa aaacacatca tattccggtt gatgagttct
    28321 acactttaaa gcgagcagaa cgcggaaatc tagtaaaaga acagttggct gaagagaaaa
    28381 atattccaca atatttgcgt tcacactggc agaaagagac ggggtttgcc tttccttccg
    28441 gtcacacgat gtttgctgcc agttgggcac tgctggccgt tggtttgctg tggccgcgtc
    28501 ggcgaacgtt aaccattgct atcttgttgg tctgggcaac gggagtcatg ggaagccgcc
    28561 tgctactcgg gatgcattgg ccacgcgatc tggtggtagc tacgttgatt tcgtgggcgc
    28621 tggtggcggt ggcaacgtgg cttgcgcaac gaatttgtgg gccattaaca ccacctgcgg
    28681 aagaaaatcg cgaaattgca caacgagaac aagaaagtta acgctggttg attttccgaa
    28741 tttaggcctt aaatcatcaa caatgcgcgt ggatgccatt tcgcagacgg cgcgaaaatg
    28801 gtactttaaa gggctattgc ggtaagttga ccataattta ttcgctctaa ccacataacg
    28861 ggaagtaatg tgaaatattt actcattttc ttactggtgt tagcgatctt cgtgatttcg
    28921 gtcacgttgg gtgcgcagaa cgatcaacag gtgacgttta attatctgtt agcgcaaggg
    28981 gagtaccgta tttccacatt gctggcggta ttgtttgctg cggggtttgc tatcggttgg
    29041 ttgatttgtg gcctgttctg gctgcgagtt cgtgtttccc tggcgcgcgc tgaacgtaaa
    29101 ataaagcgac tggaaaacca gctttcaccc gcgactgacg tggctgtagt gccgcactcg
    29161 tcagcggcga aggaataact ttctatgctg gagttgttgt ttctgctttt gcctgtagcc
    29221 gctgcctatg gctggtatat gggccgcaga agtgcgcaac aaaacaagca agatgaagcc
    29281 aaccgcttgt cgcgtgatta cgtagcgggg gttaacttcc tgcttagtaa tcaacaggat
    29341 aaagcggtag acctgtttct cgatatgctt aaagaggata caggcaccgt tgaagcccac
    29401 cttacgctcg gaaacctgtt ccgttcgcgt ggcgaagttg atcgcgctat tcgcatccat
    29461 cagaccctaa tggaaagcgc ctcgctgacc tatgaacagc gtctgttggc gattcaacaa
    29521 ctggggcgtg attacatggc cgccgggtta tacgaccgcg cggaagacat gttcaatcag
    29581 ctgaccgatg aaactgactt ccgcattggc gcgctgcaac agttgctaca aatctaccag
    29641 gctaccagtg agtggcagaa agcaattgat gttgccgaac gcctggtgaa gctgggtaaa
    29701 gataaacagc gcgtcgaaat tgcccatttc tactgtgagt tagccctgca gcatatggcc
    29761 agcgacgatc tcgatcgtgc catgaccttg ctaaaaaaag gggcggtggc agataaaaac
    29821 agcgcccgcg tatccatcat gatgggacgc gtgtttatgg cgaaaggaga atacgccaaa
    29881 gccgtcgaaa gtttgcaacg cgtcatatcc caggacagag aactggtcag cgaaacgctg
    29941 gaaatgttgc aaacctgcta tcagcagttg ggtaaaactg ccgaatgggc agaattcctg
    30001 caacgcgcgg tggaagagaa caccggtgcc gatgctgaat tgatgcttgc tgatatcatc
    30061 gaagcgcgcg acggtagtga ggctgcacag gtctatatta cacgccagct tcagcgtcat
    30121 ccgaccatgc gtgtattcca taagctaatg gattaccact taaatgaagc ggaagaaggg
    30181 cgcgccaaag agagtctgat ggtgctgcgt gacatggttg gcgagaaggt gcgtagtaag
    30241 cctcgttata gctgccagaa atgtggtttt accgcataca ccctctactg gcattgtccg
    30301 tcttgtcgtg cctggtcaac tattaaaccg attcgcggtc ttgatggcct gtaattttta
    30361 aaaaaatccg actttagtta caacatacta gttattgatg tcccattgtg ctccggcaat
    30421 gacggagcag aaaaagcctg ctaggggaac aatcgcaact gttaattttt tatttccacg
    30481 ggtagaatgc tcgccgttta cctgtttcgc gccacttccg gtgcccatca tcaagaaggt
    30541 ctggtcatga cgttaactgc ttcatcttct tcccgcgctg ttacgaattc tcctgtggtt
    30601 gttgcccttg attatcataa ttgtgatgac gcgctgtcct ttgtcgacaa gatcgaccca
    30661 cgcgattgtc gtctgaaggt cggtaaagag atgtttacat tgtttgggcc acagtttgtg
    30721 cgcgaacttc aacagcgtgg ttttgatatc tttcttgacc tgaaattcca cgatatcccc
    30781 aacactgcag cgcacgctgt cgctgctgca gctgacttag gcgtgtggat ggtgaatgtt
    30841 catgcctccg gtggggcgcg tatgatgacc gcagcgcgtg aggcactggt tccgtttggc
    30901 aaagatgcac cgcttttgat tgctgtgaca gtgttgacca gcatggaagc cagcgacctg
    30961 gccgatcttg gcgtgacact gtcacctgca gattatgcag aacgtctggc agcactgacg
    31021 caaaaatgtg gccttgatgg tgtggtgtgt tctgctcagg aagctgtgcg ctttaaacag
    31081 gtattcggtc aggagttcaa actggttaca ccgggcatcc gtccgcaggg gagtgaagct
    31141 ggtgaccagc gccgcattat gacgccagaa caggcgttgg cggctggtgt tgattatatg
    31201 gtgattggtc gcccggtaac gcaatcggta gatccagcgc agacactgaa agcgatcaac
    31261 gcctctttac agcggagtgc atgatgagtg attccaacag ccgtctggtc tactcaacgg
    31321 aaaccgggcg tattgatgag cccaaagcgg ccccagtacg ccctaaaggc gatggagcgg
    31381 tgcgtattca gcgtcagacc agtggacgta aaggtaaggg cgtttgcctg attaccggta
    31441 tcgatctcga tgatgccgaa ctgacaaaac ttgcagcgga actgaagaaa aaatgcggct
    31501 gcggcggagc agttaaagat ggagttattg aaatccaggg cgataagcgt gatttattaa
    31561 aatcactact tgaagcgaaa gggatgaagg taaaactcgc aggcggttaa cataaaaagc
    31621 cacggatata tccgtggctt tcgaatattt tactgtgtgt attattcagt tatttcgtac
    31681 tgaacagaa
//
LOCUS       DAETPZ010000043        29734 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.043, whole genome shotgun sequence.
ACCESSION   DAETPZ010000043 DAETPZ010000000
VERSION     DAETPZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.043"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            348..641
                     /locus_tag="K0W59_002209"
     CDS             348..641
                     /locus_tag="K0W59_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HBI3023232.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="K0W59_002210"
     CDS             713..1372
                     /locus_tag="K0W59_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBI3023233.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="K0W59_002211"
     CDS             1464..1910
                     /locus_tag="K0W59_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HBI3023234.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="K0W59_002212"
                     /pseudo
     CDS             complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="K0W59_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3390..3809
                     /locus_tag="K0W59_002213"
     CDS             3390..3809
                     /locus_tag="K0W59_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBI3023235.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3809..5077
                     /gene="umuC"
                     /locus_tag="K0W59_002214"
     CDS             3809..5077
                     /gene="umuC"
                     /locus_tag="K0W59_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HBI3023236.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSTSVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="K0W59_002215"
     CDS             complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="K0W59_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HBI3023237.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="K0W59_002216"
     CDS             complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="K0W59_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HBI3023238.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREGVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7561..8280
                     /gene="fadR"
                     /locus_tag="K0W59_002217"
     CDS             7561..8280
                     /gene="fadR"
                     /locus_tag="K0W59_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HBI3023239.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8332..9864)
                     /locus_tag="K0W59_002218"
     CDS             complement(8332..9864)
                     /locus_tag="K0W59_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HBI3023240.1"
                     /translation="MATIDSINKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARHYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10194..11492
                     /gene="dadA"
                     /locus_tag="K0W59_002219"
     CDS             10194..11492
                     /gene="dadA"
                     /locus_tag="K0W59_002219"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HBI3023241.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11502..12572
                     /gene="dadX"
                     /locus_tag="K0W59_002220"
     CDS             11502..12572
                     /gene="dadX"
                     /locus_tag="K0W59_002220"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HBI3023242.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12740..14166)
                     /locus_tag="K0W59_002221"
                     /pseudo
     CDS             complement(12740..14166)
                     /locus_tag="K0W59_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14776..16512)
                     /locus_tag="K0W59_002222"
     CDS             complement(14776..16512)
                     /locus_tag="K0W59_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBI3023243.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16607..17521)
                     /gene="ldcA"
                     /locus_tag="K0W59_002223"
     CDS             complement(16607..17521)
                     /gene="ldcA"
                     /locus_tag="K0W59_002223"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HBI3023244.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            17621..18232
                     /gene="emtA"
                     /locus_tag="K0W59_002224"
     CDS             17621..18232
                     /gene="emtA"
                     /locus_tag="K0W59_002224"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HBI3023245.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLRYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18234..18968)
                     /locus_tag="K0W59_002225"
     CDS             complement(18234..18968)
                     /locus_tag="K0W59_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HBI3023246.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19169..19423
                     /locus_tag="K0W59_002226"
     CDS             19169..19423
                     /locus_tag="K0W59_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBI3023247.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19473..20933)
                     /locus_tag="K0W59_002227"
     CDS             complement(19473..20933)
                     /locus_tag="K0W59_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBI3023248.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20870..21426)
                     /locus_tag="K0W59_002228"
                     /pseudo
     CDS             complement(20870..21426)
                     /locus_tag="K0W59_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
     gene            complement(21452..22306)
                     /locus_tag="K0W59_002229"
     CDS             complement(21452..22306)
                     /locus_tag="K0W59_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HBI3023249.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22306..23115)
                     /locus_tag="K0W59_002230"
                     /pseudo
     CDS             complement(22306..23115)
                     /locus_tag="K0W59_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(23125..23883)
                     /locus_tag="K0W59_002231"
     CDS             complement(23125..23883)
                     /locus_tag="K0W59_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI3023250.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APIIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23880..24860)
                     /locus_tag="K0W59_002232"
     CDS             complement(23880..24860)
                     /locus_tag="K0W59_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBI3023251.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVSV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGELMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24860..25882)
                     /locus_tag="K0W59_002233"
     CDS             complement(24860..25882)
                     /locus_tag="K0W59_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HBI3023252.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWLPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26948..27388
                     /locus_tag="K0W59_002234"
     CDS             26948..27388
                     /locus_tag="K0W59_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023253.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(27467..29164)
                     /gene="treA"
                     /locus_tag="K0W59_002235"
     CDS             complement(27467..29164)
                     /gene="treA"
                     /locus_tag="K0W59_002235"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBI3023254.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLGTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(29484..>29734)
                     /locus_tag="K0W59_002236"
     CDS             complement(29484..>29734)
                     /locus_tag="K0W59_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="phosphotransferaese subunit; phosphorylates
                     dihydroxyacetone along with DhaK/DhaL; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HBI3023255.1"
                     /translation="PQFNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALI
                     ARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFNRQG"
BASE COUNT         7456 a         7226 c         7638 g         7414 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgtga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcctgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc ttcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgttttatct
     3001 gcaacgattt cagtcataat cattcgcctc tttaaatata taaattgtaa tgaaactcct
     3061 gttttacaac tattaataaa ttttacttca tctaattcat agttagccgg gcgggatgcg
     3121 tcaatgtctt tatttctatt aatatgataa atatcaaaca atgtttaatg tcattatggc
     3181 gaatgcttct attctatttt tttgccgggt gatatttttc atttctgctg gatgagcgtc
     3241 gtcgccagaa ggccacgtga gcacaggata agagaacgaa aaatcagcag tctatgcagc
     3301 gacaaatatt gatagcctga atcagtattg atctgctggc aagaacagac tactgtatat
     3361 aaaaacagta tagcttcagg cagattatta tgttgtttat caagcctgcg gatctccgcg
     3421 aaattgtgac ttttccgcta tttagcgatc ttgttcagtg tggctttcct tcaccggcag
     3481 cagattacgt tgaacagcgc atcgatctga atcaactgtt gatccagcat cccagcgcga
     3541 cttacttcgt caaagcaagt ggtgattcta tgattgatgg tggaattagt gacggtgatt
     3601 tactgattgt cgatagcgct attaccgcca gccatggtga tattgtcatc gctgctgttg
     3661 acggcgagtt tacggtgaaa aaattgcaac tacgcccgac ggtacagctt attcccatga
     3721 acagtgcgta ctcgcccatt accatcagta gcgaagatac gctggatgtc tttggtgtgg
     3781 tgatccacgt cgttaaggcg atgcgctgat gtttgccctc tgtgatgtaa acgcgtttta
     3841 tgccagctgt gagacggtgt ttcgccctga tttatggggt aaaccggtgg ttgtgctatc
     3901 gaataatgac ggttgcgtta tcgcccgaaa cgctgaggca aaggcgctgg gcgttaaaat
     3961 gggcgatccc tggttcaaac aaaaagatct gtttcgtcgc tgtggtgtgg tttgctttag
     4021 cagcaattat gagctttacg cagacatgag caatcgggtg atgtcgacgc tggaagaact
     4081 atcgccccgc gtcgagattt acagtattga tgaagcattc tgcgatctga caggtgtgcg
     4141 taattgtcgc gatctgactg attttggcag agaaattcgc gcaacggtgc tacaacgtac
     4201 ccatcttact gttggtgtgg ggatcgccca gaccaaaacg ttggctaagc ttgccaatca
     4261 tgcggcaaaa aaatggcagc ggcagacggg tggggtggtg gatttatcaa atctggaacg
     4321 ccagcgtaaa ttaatgtctg ctctccccgt ggatgacgtc tgggggattg gacggcggat
     4381 cagcaaaaaa ctggacgcga tggggatcaa aaccgttctc gatttggcgg atacagatat
     4441 ccggtttatc cgtaaacatt ttaatgtcat gctcgaaaga acggtgcgtg aactgcgcgg
     4501 cgaaccctgt ttgcaactgg aagagtttgc accgacgaag caggaaatta tctgttcccg
     4561 ctcgtttggt gaacgcatca cggattatac gtcgatgcgg caggccattt gtagttacgc
     4621 tgcccgggcg gcggaaaaac ttcgcagtga gcatcaatat tgtcggttta tctccacatt
     4681 tattaagacg tcaccatttg cgctcaatga accttattac ggcaatagca cgtcggtaaa
     4741 actgctgacg cccactcagg acagcaggga tatcattaac gctgctacgc gatctctgga
     4801 tgccatctgg caagcgggcc atcgctacca aaaagcgggc gtgatgctgg gggatttctt
     4861 cagtcaggga gtcgcgcagc tcaatttatt cgatgacaat gcaccgcgcc ccgggagtga
     4921 gcaattgatg gcggtaatgg atactctgaa tgctaaagag ggcagaggaa cactctattt
     4981 tgccgggcag gggatccaac agcaatggca gatgaagcga gctatgcttt caccacgtta
     5041 tacaacgcga agttctgatt tactgcgggt caaataaata tagcggcagg aaaaaagcgt
     5101 tcccgcagga gcgccgaatg gattagcgac cgaacagatc acgttttttc gctttaaacg
     5161 gctgggaaat caccaccagc accgcgacaa tcaggtaagc gataaaaata ccgagcagcc
     5221 actgcggcat ttccatacct aaaaaatccc actgacgctc ggcgcaatcg ccagaggcga
     5281 caaacacttg cggcacccat ttatccagcg gcagccattc cgggaaacga accataaaat
     5341 cacaggtggc aaatggcgaa ggatagagct gaagcatggt gtgctcgtaa gttaactgca
     5401 caccgcggaa cgcactatac aaccagataa ccatcgctac ataacgcagc ggagttttcg
     5461 gggcgatcgc gccaatcagc gcagcaccca gaacgccgaa taacgcgcag cgttcataaa
     5521 tacagagcac gcaaggtttc agtaacatca catgctggaa ccacagcgcc gtcagttcca
     5581 gtgccagagc agtaaacgcc atcaacagcc acgcgccccg gccttgtgaa cattggttca
     5641 aaaatcgcaa cataatcatt tccctgcaat atgcatagag tgcgcagttt aaaccaattc
     5701 attcgctgcg ccaccaggag gcagcaaaaa taggacgcaa ttggacaatt atcgggcgaa
     5761 aaggcaaacc gggcatttat atgcccggta agttgttatc aaagcgttgc tatccagccc
     5821 atttgcataa accattcggt tacaggggca agcgtaaact cgacgcagag caagccgacg
     5881 agtgtcagta cgagggtgta aggcagggcc atccacacca tgcggccata agagaggcga
     5941 atcaatggcg cgagtgcaga ggtcagcagg aacaggaacg cagcctgacc gttcggcgta
     6001 gcgacagagg gcagattggt accggtatta atggcgaccg ccagcagctc gtattgcttc
     6061 aacgtgatag cgccactttc cattgccgct ttcgcttcgt tgatataaat cgtccccacg
     6121 aagacgttat ccgaaatgga tgacagcaga ccgttgaaaa tatagaacag cgacagctga
     6181 gcatgttcag atgcctgcaa cacaaactga ataatcggcg aaaacagttg ttggtcgata
     6241 atcaccgcga ctaccgagaa aaacaccgtc aacagtgcgg tgaatggcag agattcggtg
     6301 aaggctttac cgatagcatg ttcatcggtg acaccggtca atgatgttgc cagaataatg
     6361 actgacaaac caatcaagcc aacttccgcc agatgcaacg ccagcgcagt caccagccag
     6421 acgccaataa tcgcctggac aatcagacgg attttatcct gacgggtacg ctggtggcgg
     6481 ctttgatcgt caaactgttg caacacttcg cggactttct ccggcagcgt ttcaccgtag
     6541 ccaaaccaac gcagcttctc taccagcagg caggttaaca ggccacaaat cagaaccgga
     6601 acggtcaccg gcgacatgcg caggaagaaa tcgccaaaat gccagccagc cgctttagcg
     6661 atgatcaggt tctgtggttc gcccaccatg gtcattacgc cgcctaatgc ggtgccgaca
     6721 ccggcatgca tcatcaggct acgcagaaag ccacggaact gttccagaac cacgttgtaa
     6781 tgcttgtcga tatgactatc gtcttgcagg tcggtgtctt cggtacggga agaggctacg
     6841 cgatgataaa taccataaaa accgactgca acgctgatca ccaccgccac gacggttaag
     6901 gcatcgagga acgcggagag gaacgctgcc gccacgcaaa aagagagcga cagcagcatt
     6961 ttggagcgaa tgcttaacag caaacgggta aatatgaaca gcaacagctg tttcataaaa
     7021 tagatacccg ccaccataaa catcaataac agcaagactt caagatttgc cgccacccct
     7081 tcacggacgt gttccgcgct ggtcatgccg atgaataccg cttcgatagc caacagacca
     7141 ccggggagta gcgggtagca tttcagggcc atcgccagag tgaaaataaa ttccgctacc
     7201 agcaaccagc ccgcgacgaa agggctgatg aggaaaatta acgggtttac gattaagaaa
     7261 atgatgaggg cgagtttgta ccagtcgggg gactggccca aaaaattgcg ccatagcgcg
     7321 cggccccagg agatctccat gatggtttcc cttaccttac aaataatcaa tgatgttttt
     7381 gtgtttaaac gcaaagctta acggtcaggc aggagtgagg caagtcttga tagtcaaggg
     7441 gaaagagatg cggaaaatga agccttgatc cctttttctt ctttttgtct gctatcagcg
     7501 tagttagccc tctggtatga tgagtccaac tttgttttgc tgtgttatgg aaatctcact
     7561 atggtcatta aggcgcaaag cccggcgggt ttcgcggaag agtacattat tgaaagtatc
     7621 tggaataacc gcttccctcc cgggactatt ttgcccgcag aacgtgaact ttcagaatta
     7681 attggcgtaa cgcgtactac gttacgtgaa gtgttacagc gtctggcacg agatggctgg
     7741 ttgaccattc aacatggcaa gccgacgaag gtgaataatt tctgggaaac ttccggttta
     7801 aatatccttg aaacactggc gcgactggat cacgaaagtg tgccgcagct tattgataat
     7861 ttgctgtcgg tgcgtaccaa tatttccact atttttattc gcaccgcgtt tcgtcagcat
     7921 cccgataaag cgcaggaagt gctggctacc gctaatgaag tggccgatca cgccgatgcc
     7981 tttgccgagc tggattacaa catattccgc ggcctggcgt ttgcttccgg caacccgatt
     8041 tacggtctga tccttaacgg gatgaaaggg ctgtatacgc gcattggtcg tcactatttc
     8101 gccaatccgg aagcgcgcag tctggcgctg ggcttctacc acaaactgtc ggcgttgtgc
     8161 agtgaaggcg cgcacgatca ggtgtacgaa acagtgcgtc gctatgggca tgagagtggc
     8221 gagatttggc accggatgca gaaaaatctg ccgggtgatt tagccattca ggggcgataa
     8281 tcccttccgt ttaaagagca aacccctcaa acgaggggtt ttttgttgtt tttacagatt
     8341 tcccattctt ggcgggcaac gttccagcaa ctcgatgctg ccgtcttcgt tttgctgttc
     8401 gagcatcaca tcaaatcccc acaggcgatg cacatgcttc aggacttctt tgcgcccccg
     8461 atccagcggt gcgcgattat gtggaatata acgcagcgtc agcgaacggt cgccgcgcaa
     8521 atccacgttc cagatctgaa tattcggctc cagattactt aagttatatt gcgacgataa
     8581 ccggttacgg atctcccgat aaccttcttc attatgaata gcggaaatct ccagataatt
     8641 atgccgatca tcatccagca cggtgaagaa gcggaaatca cgcatcactt tcggtgacag
     8701 aaactggctg ataaaactct catctttgaa atcacgcatc gcgaaatgca gcgtttccag
     8761 ccagtcggaa ccggcgatat ccgggaacca gtatttgtct tcttccgttg gcgactgaca
     8821 aatccgttta atatcctgga acatggcgaa cccgagggca tacgggttga tgccgctgta
     8881 ccacgggctg ttataggggg gctggaagac cacattggtg tggctgtgca aaaactccag
     8941 cataaaacgt tccgttactt tcccttcatc atacagatgg ttaaggatgg tatagtgcca
     9001 gaaggtcgca cagccttcgt tcatcacctg agtctgtttt tgcggataaa aatactggct
     9061 caccttacgc acaatacgca ggatttcacg ctgccatgat tccagcagtg gcgcattttt
     9121 ctccataaaa tagagcaggt tttcttgtgg ttcggacgga tagtggcgcg cttcagcaac
     9181 cgttttctct tcctcgcgct tcggcagggt acgccagagc atattgacct gactttgtag
     9241 atactcttcg cgacttttct gccgggcttt ctcttcttgc agcgagattt tttgcgggcg
     9301 tttgtaccgg tccacgccgt agttcatcag cgcgtggcac gagtccagaa gccgttctac
     9361 ttcatcaacg ccataacgtt cttcgcactc ggtaatatat ttacgggcaa aaatcagata
     9421 atcgacaatc gaactggcgt cggtccaact gcggaataag caattgtttt taaagaaaga
     9481 gttatgccca tagcaggcgt gtgccatcac cagcgcctgc atagtaatgg tgttctcttc
     9541 catcaagtaa gcgatacacg ggttagagtt aatgacgatt tcataggcca gcccttgctg
     9601 accgtgctta tacagccgtt cagtctcgat aaacttttta ccgaatgacc agtgcggata
     9661 gttaattggc atgccgacgc tggaataggc atccatcatc tgttctgagg ttatcacttc
     9721 aatctggtgc gggtaggtat ccagccggta gagtttcgcc acccggtcta tctctgccag
     9781 ataaacatcc agcaggtcga acgtccagtc gggtccatcg ctcaaacgtg tggtgtcctt
     9841 attaatagaa tcgatcgtcg ccatacgcgc acctcattgt tgtcggcgct ctctgtgtgg
     9901 agcacctcat ttcaagcata gaacacctgt taaaaaccgc gtcgccggag aatttttttc
     9961 tttgcgattt cttattatca gagtgccact aatccgcttc tgaacggaat tttatgctgg
    10021 ataaaaaggg cgttcagcag gagatactaa agacgccata ttgccgcaga gtcagggaga
    10081 tgtgagtcag ctcaccataa aaaagccgta tgttgaataa tattttcaac tgagttatca
    10141 agatgtaatt agattattat tcttttactg tatctaccgt tatcggagtg gctatgcgag
    10201 ttgtcatact gggaagtggt gtggtaggcg ttgccagcgc ctggtactta aatcaggcag
    10261 gacatgaggt caccgtcatt gatcgggagc cgggggccgc cctggaaacc agtgccgcga
    10321 atgccgggca aatctccccc ggatatgctg caccgtgggc agcaccaggt gtgcctttaa
    10381 aagcgattaa atggatgttc cagcgccatg cgccgctggc ggttcgtctc gacggtacgc
    10441 agtttcagtt gaaatggatg tggcaaatgt tacgtaactg cgacaccagc cactacatgg
    10501 aaaacaaagg gcggatggtg cgtctggcgg aatacagccg tgattgcctg aaagcattgc
    10561 gcgccgaaac caatattcag tatgaagggc gtcagggtgg gacgctgcaa ctgttccgta
    10621 ccgaacaaca gtatgaaaat gcgacccgcg atatcgccgt gctggaagat gccggcgtac
    10681 cgtatcagct gctggaatcc agccgcctgg cggaagtgga tcccgcgctg gcagaagtag
    10741 cgcacaaact gacgggcggc ctgcagttac ccaatgatga aaccggagac tgccagctgt
    10801 ttacccagaa tctggcgcgg atggcagagc aggcgggggt taaattccgc tttaatacgc
    10861 ccgttgacca actgctttgc gacggcgagc aaatctacgg cgtgaagttt ggcgatgaag
    10921 tgattaaggc cgatgcgtat gtgatggcgt ttggttctta ctcgacggcg atgctcaaag
    10981 gcattgttga tattccggtg tatccgctga aaggctactc gctgactatt ccaattgcgc
    11041 aggaagatgg tgcgccagta tccaccattc ttgatgaaac ctacaaaatc gccattaccc
    11101 gtttcgataa ccgcattcgt gttggcggaa tggcggaaat tgttggtttt aacaccgagc
    11161 tgttgcaacc gcgtcgtgaa acgctggaaa tggtggttcg cgatctctat ccacgcggcg
    11221 gtcatgtcga gcaggcgact ttctggacgg gtttgcgccc gatgacgcca gacggtacgc
    11281 cggttgtcgg gcgtacacgc tttaaaaatc tgtggctgaa taccggtcac ggcacgctcg
    11341 gctggacgat ggcttgcggt tccggtcagt tgttaagcga tctgctctct ggtcgcacgc
    11401 cagcgatccc atatgaggat ctaagcgtag cgcgctacag ccgtggattt acgccatcac
    11461 gtccgggcca tttacatggc gcacacagct aaggaaacga gatgacccgt ccgatacagg
    11521 ccagcctcga tctgcaggca ttaaaacaga atctgtccat tgtccgccag gccgcgccgc
    11581 acgcgcgcgt ctggtcggtg gtaaaagcga acgcttacgg gcatggtatt gagcgtatct
    11641 ggagcgcgct cggggccacc gatggctttg cattacttaa cctggaagag gcaataacgt
    11701 tacgtgagcg cggctggaag gggccgatcc tgatgctgga aggatttttc catgctcagg
    11761 atctggagat ttatgaccag caccgcctga ccacctgcgt acacagcaac tggcagctca
    11821 aagcactgca aaatgcgcgg ctaaaagcac cgttggatat ttatcttaaa gtgaacagtg
    11881 ggatgaatcg gttgggcttc cagcccgatc gcgtgcttac cgtctggcag cagttgcggg
    11941 caatggcgaa tgttggcgaa atgaccctga tgtcgcattt tgctgaagcg gaacatcctg
    12001 atggaatttc cggcgcgatg gcgcgtattg agcaggcggc ggaggggctg gagtgtcggc
    12061 gttcgttgtc caattcggcg gcgactctgt ggcacccgga agcgcatttt gactgggttc
    12121 ggcctggcat tattttgtat ggcgcttcgc cgtccggtca gtggcgtgat atcgccaata
    12181 ccggattacg tccggtgatg acgctaagca gtgagattat tggtgtccag acgctaaaag
    12241 cgggcgagcg tgtgggctac ggcggtcgct atactgcgcg cgatgaacag cgaatcggca
    12301 ttgtcgccgc aggatacgcc gacggttatc cgcgccatgc gcctaccggt actcctgttt
    12361 tagtggacgg cgtgcgcacc atgacggtgg ggaccgtctc gatggatatg ctggcggttg
    12421 atttaacgcc ttgcccgcag gcggggattg gtacgccggt tgagctgtgg ggcaaggaga
    12481 tcaaaattga tgatgtcgcc gccgctgccg gaacggtggg ctatgagttg atgtgcgcgc
    12541 tggcgttacg cgtcccggtt gtgacagtgt aacttattgt aagccggatc ggaggcagcg
    12601 tcttctggtt gcgaaaaaat catccatccg gctggtcagc aactgtagtt gttaatgtga
    12661 cagagccatt gcccatgata gtgtccatta aaagaatgga cactatttcc ccggaacctg
    12721 aactcgccgc acaggcgttc tacataaaac gcttacgctt cattattgac tcggagtcga
    12781 cttagataaa tcacgcttca cccttgatgc agattcgggt tcatgttcag attgttgatt
    12841 aacaggctgc gagtcctgtt gtctttccag actcaacagg cccaactgtt gatccaggag
    12901 ctccaggtcc tcctgtacct tcacgcgctc caggagttcc gggtcatcca ggaactccag
    12961 atccataagg tcctccagct cctcgagtcc ttccaggtca acctagcctt ccttgtgcaa
    13021 aaagtccaaa aggtccttca ggccctgctg ttttttcagg ccctcgacgg ctttgagcag
    13081 cgtcggtaaa ttgtctttgt cccacgggtt accattacag tcaaacacct tcaggctctg
    13141 gggctgcggc acctgtaccg ttttaaactt gttttctcca cagtagtaaa ttattagttt
    13201 gggcggcagc cgtggtgaag agatcaaatc gttactagaa cagtcaaata ctttcaggcg
    13261 agtcaagttc tccagcatct catcaggtat tgagttaata ttgttgccgg cacaggatag
    13321 tttatacagg tactggggca tcgacggcgg taaaaattgc aagaagtttt cagaacagtt
    13381 aatcactctc aggttagtcg acagctcagg cagccagata atacagtttt catagcactt
    13441 cagtaccatc agttttgagg gctgcacctt cggtaacaag atcaagacgt tatctgtaca
    13501 gtcaagcacc agcagccact ttggcatcac cttcggtaac gcggtcaaca cgtttttact
    13561 gcagtaaaat tcaatcagat gtggaggcaa attctcaggg agtgaagtca atcccaggtt
    13621 gctaagattg agcatactcc gtcttgtaat gagacattcc ttcattctgc gcaatgcttc
    13681 tgcccggttt tcaatccggc ttgctggcag gttttcttcc aggatatttt ccttgatttc
    13741 ttccagactt tctttcaggt cttcttgggg aatttctgac tgtttgtttt gctggttttc
    13801 ttccaggatt tcgtacaggt ttttttgcag gttttcttgc aaaccatcct gctgtatagc
    13861 ccattcgcac catttggcga ccatcatgaa ctcgttatgt tgctgattca ggcgagtccc
    13921 caacgtgatg atggtattgt tgtccacctg tatgttcact ttgcccgcga tactgaggtc
    13981 aatgatctgc cagggggcat caccccagct gaatttttgc gggctgagat acttcatgtt
    14041 ctgcaaaatt tgattcttga gttccaccga caggaagagc cgccctggcg ggcgtcgtac
    14101 gtctgggtta acggaagatg ggggaatggc gggggcgaat gggcagatgt ctttaaagct
    14161 agccataatg ctacgtttcc ttgaaataaa tatcctctgg cagcgccgga gacgtgaatg
    14221 tggtcaccgc aaaggagcca caggacgtca tttgcgctga tatcactact catcgttgac
    14281 tttttccctg gtcagaaatg ccgtgtcggg tctggtacat gctgtattac ggcctaggta
    14341 gctctgacgt attaaagaaa atacgagagt gaacgacccg gaaataatcg ttattaagca
    14401 aaggaataac tataagaaat cgctcatgca tccggtgcta caggcggatg aaacgcctat
    14461 tgtgtgggct atgtcagtgc ggcaggttgc gtggggaata tgttcaggcg atgtcaggca
    14521 ggggcccctg atggttgacc ggtaatgcgg ggctggtttt tattacctgt tacagtcgga
    14581 aaaagccctt acgctgactt tcttgagccg gtgttttgag ttcattagcc agagcctgca
    14641 atggtttgtc atccataatt taaccttctt ctgatgttgg gatgagcata tcaaaatcag
    14701 gaaatgacaa gtttattaca ggctcaggct gcgttcagta aagcatcgca tccggcgata
    14761 gatgctgcat taaatttaag attcagcttc ctcttcagcc acccgaacgc caatcttcaa
    14821 cacttcatta tcttctttct cggccaccgt ccagatcatc ccggcaaact ctacctggtc
    14881 accgacaacc ggtgccgcgc ctagcaactg ctggacaatt tcacccagcg tttgctgttt
    14941 atcacgatat tctcgcccgt cttccaggcc atatatcagc gcaacatcag catatttggc
    15001 gctggcttcg agaatgaagt caccaaagaa gcgttgatcc agcgcgaccg gtggcgactg
    15061 gctgaacagt ttaccgagcg ccgggagatc gcgttcccga ccaattacac acaatacatc
    15121 gccttcgcgc agtcgggtgc tgccggtagg atgaagcaac tggttatcac gaaacagggc
    15181 tgcaatacgc gtctcttttg gcatatgcaa atcgcgcagt gccgcaccta cacaccattt
    15241 atcagcactc aattgataaa caaactgctc ccacggattt tccggatgaa tatccaggcc
    15301 aacgcgtgac accggacgtc cgacgggcgg aaccaccact ttggcttttt tcgccgccca
    15361 cgagagtgat gttccctgca agagcagtga aaccagaacc acaaagaagg cgacattaaa
    15421 gaacagtcgc gcattctcca gccccgccat catcgggaac acggccagga tgattggcac
    15481 cgcgccgcgt aatcccaccc agctgataaa cacgcgctca cgcagattga aaccgcggaa
    15541 agggagcaat ccagcaaata ccgaaagggg acgggcgaag aatatcatcc atgccgacaa
    15601 aatgagcgcc ggaatggcaa taggcagcag atcgcttggg ttaaccaata gccccagcac
    15661 caggaacatg gcgatttgcg ccagccaggc gaggccgtcg aaattttgca ggatgccgta
    15721 gcggttgcga atcggccgat tacccagcag aaaaccgcac agataaacag ccagaatacc
    15781 gctgccttcc agcgccgtgg ttaaagcaaa aattaaaata ccgccgctta atgccagcaa
    15841 tggatataat ccggcaggca gggcgatgcg attaatcatt tgcagcagta aataaccacc
    15901 gccaagaccg atgacaatcc cgaggccaaa ttgctgcaga atatcgacca cgaacatcca
    15961 actgacactg ctttcatgct gctggatcat tgcaatcagg gtaatcgtca gaaagaccgc
    16021 cattggatca ttactgccgg attcaatttc cagcgtcgag ccaacacgtt cgttaagccc
    16081 cttaccaccc agcagagaaa agaccgctgc agcatcggtg gagccgacga tagcgccgat
    16141 taataagcct tcaatcaaat caagattaaa cagccacgcc gccatcatgc cggttaaacc
    16201 agaggtgata agtacgccca gcgtcgccag cgacagcgcc ggtcctaacg ccacacgaaa
    16261 ggaactggcc tgagtgcgca tcccgccgtc gagcaaaata atcgccagcg ccaggttact
    16321 taccatgtag gcgaaggggt aattatcaaa cgggataccg ccgacgccat cgactcccgc
    16381 cagcatgccg atcgccagaa aaataaccag aataggaatg ccaagacggg aagaaaatga
    16441 actaagtaat atactgctgg tgacaaggat ggatcctaag atgaaaaggc taattattgt
    16501 tgtggcatcc aacggtcggt tactcctgat tacgctgtct cttatataaa ccctaccata
    16561 ttagcggcag agacagcgtt ttacttagcc ctgaagcgtg atttttttac attttaagaa
    16621 caggatgacc gctaatcgtc aactgagtgc cttcccgggt gttattcaga atggcatgtg
    16681 cgcccaacgg cagcgtaacc gtgcgttgtt catgaccaaa atcgaggccg gtaatgagtg
    16741 gaatcgacag gcgggaacgc aaaaacgcgt acactgactc caggttgtaa cctgcgtcat
    16801 aatcattggg cgtgctgccg ctaaagctac cgagaataat cgccttctga cgtggcaaaa
    16861 ttcccgcatg atagagctgc aacagcatac gttcgacgcg gaaagggtgc tcgttaatat
    16921 cttccagcac cagaataccg ttctcaattt ttggcatcca cggtgtacca atcagtgaaa
    16981 tcagcatcgc aagattgcct ccccacaacg tgccttcggt ctgacatgtc gggccttccc
    17041 cttgccattc aatggtgaag gtttcattgc gtaacgccag ccagaaatgg tgctcggtaa
    17101 aggcgttcag ttcatccgca ccaaagtttg ctaccagcat ggggccgcta aaggtgatga
    17161 cattgccttg cgccagaaga ccgcactgaa tggcggtaaa atcgctatgt ccgcaaatga
    17221 gtaacgggtc atgttgttgg cgagccacca gtgcctgcca gtcaatatct gccagtaaac
    17281 gactggcacc gtaaccgccg cgtacagcca gcacgatggt gttgggggta gtcagtctgg
    17341 caagggaatt gagatcttcc agacgctccg tttccgtacc cgcaaaacgc tcacaacgac
    17401 gggcaataac ctcgacgtta ttgacttgat gccccgcgtc ggtaaggcgc tggataccac
    17461 gcagcgcggc gtgctgttta atgctgtaac ccgatggggc aattaagtga aacagagaca
    17521 tggcaattcc ttgctgacaa cagaaacgaa atgtatatca tgccgcttag gtgtgccgtt
    17581 gtcacctcaa cggcgattcc aggctataag gatagaagaa gtgaaattga gatggtttgc
    17641 ctttttgatt gtgttattag cgggttgttc atcaaagcat gactatacga acccgccgtg
    17701 gaacgcgaaa gttccggtgc aacgtgcgat gcagtggatg ccaataagcc agaaagccgg
    17761 tgcagcctgg ggcgtcgatc cacaattgat cacggcgatt atcgctatcg aatcgggtgg
    17821 taatccgaac gcggtgagta aatcgaatgc cattggtttg atgcagttaa aagcttcaac
    17881 ctccggacgt gatgtttatc gccgtatggg ctggagtggt gagccgacga ccagcgagct
    17941 gaaaaatcca gagcgtaata tttcaatggg ggcggcttac ctgaatattc tggaaaccgg
    18001 cccgctggca ggcattgaag atccgaaggt actgcgatat gcgctggtgg tgtcatacgc
    18061 taacggggca ggtgcgctgc tacggacttt ctcgtcagat cggaaaaagg cgatcagcaa
    18121 aatcaacgat ttggatgctg atgagttcct cgatcacgta gcgcgaaatc atcctgcgcc
    18181 gcaggctccg cgctatatct acaaacttga gcaggcactg gacgcgatgt aaatcagtcg
    18241 cgcactttgt ccgctttttc ccgtgcttct cgctcgagag agaaaataat ccgctgtaat
    18301 tgccgctcca ccgtcgggct gacgttaaga aaacggaagc tcagacgggg agtggtgatg
    18361 gtttcattct tgccatcaat cactttgcgc tcgctggtgg agattaactg ggcgtcaaag
    18421 tgaaaaacac cccattgccc catgttgact tcaatttgag cgaagcgcat accttcttgt
    18481 aattcggcag gctttgctgt ttccagtaat gcgcccatgc cgcctaacga caaatcatac
    18541 aagcggaaac gtaacgtact gttatccgcc agtttggtct ggcaaaaata aggcggatgg
    18601 agtggggcgg agatgcggaa atatcggcgt cgttgtacaa accataaggt gggaggcggt
    18661 acggtaataa atgccggaag ctgcaagtat tcactctgct gtagttgttc aacagtaaac
    18721 tcgactttcg caccctgagt ttcggcggta atggtaatgt gctgtgcctt tagcacggcg
    18781 atgttgtctt cggcttgact gccgaaatcc agcaccagtt tatccggggt tattgccagt
    18841 attttgctga tcagctgccc gccattccaa ctgagacgca aaggaattgc ggctttgtgc
    18901 aaatcgcgta acacgcccag gacggctaac ggattttgtt tcaggaactg ctcatggtaa
    18961 tgactcacgc ggaaaaactc ctgactgaca aactgtcttt ggtttatcgg tcatagttaa
    19021 caaaacttaa tacaaatgcg tgaatatttt ttacatgttg ttcttaaatc agccgacaaa
    19081 cgcccagcat cgatcccgcc cttgcctata cttagagcgt tgacgtaagc atttcttgcg
    19141 accgattcat cgaaacgagg gcctgaacat gggaattatt gcctggatta tttttggcct
    19201 gatagccggc attatcgcca agctaatcat gccggggcgt gatggtggtg gatttttcct
    19261 gacctgtatt ctcgggatag tcggtgcggt ggtcggcggc tggctggcga ccatgtttgg
    19321 cattggcggc tccatcagcg gttttaattt gcacagcttc ctggtggcgg tggtgggcgc
    19381 tatcctggtg ctgggcgtat tccgcctcct gcgaagagaa taagattttc ataaggcgga
    19441 tagcgataca gatgccgcta tccgctttca catcagaacg tatactcgac acctgcccag
    19501 aaattgcgac cttcttcaat aaacccttca ctgtaggcat attgggtatc aaacaggtta
    19561 ttgaccgacg cattgacgct gaagccatga cctaaggtgt aatcggctcg aatgtgggtc
    19621 accgcaaaac cggcggcttt ttgtgaaccg tcactgttgc tgtagctgga ggaacgcgcc
    19681 tcttccgaca gcgttacgct taacggctcc catggtttga gtgtcatcca tgcggtcatt
    19741 gtctgcgttg gaagatcggt tatcttgccg atgtctttgc gtttggcgtc agcgtggatc
    19801 agagcgtagc tcaatcctac atccagtata ttgctgattt tccccttaat accggcatcc
    19861 agaccgctgt aatccaccgt gccgctgttc tgattttgaa tggtatcggc attgatattg
    19921 tgcgagagga tggcatcact cacccggtta tagtaaacgc tgacctcaaa tccccagtcg
    19981 cgcgtgaagg caccattcca ggttaaatcc accccgcgag cgcgttccgg cttgagctgt
    20041 gggttaacaa tcgctatctg gttatacgca ggtttggatg tggtatagcg ttctttcagc
    20101 gtcggaaagc gtgtgcggtc atagtacgaa agcgccagag tgtcttcatt ggcaaagtgg
    20161 tatttcccca tcacctgcca gttaaaagct gactgattgt tgtcgtcata atgggtgatg
    20221 ctgccatctt tctcgtgttt tttcgcttct acgctatcgc gccagtcata gctgattccc
    20281 gccacgacat cgacattatc agcagcagcc cattgatatt cactggcgag cgaccaggta
    20341 cgatcttcat agcgatcgta agcggcgtgc ggcgcacctt tttcccggtg tacgtcatct
    20401 ttccagttaa cggcaaacga cagcagatcg ttttcacgca catcggctgc cagttgtaat
    20461 ccggcaccgt cgctgtaatc ggaataatgg ctgtagctgc cttttttatt tttcaaatca
    20521 gccagcgagt tgtacatcat cagcgtattt tcaaaggtgt cgcgatacag ccgacttttc
    20581 agggtaaaac ggtcgtttag ttgggtcgct ccctgataat aaaaactttc tttgtcatac
    20641 tctggccact gccagtagcg tgatttttga ccacaatttc cgctgtatgg cgggttatct
    20701 ttttcaccat cctgcttaat gtaagtcagc gtgtattcat cgttttcgcg cggtgtaaaa
    20761 ccgagcttca caatgccgcg tttatcatca gccgatgaat taatcatctt gccgtgtttg
    20821 cctgcaatat cattatttac accatgcggc aggccgagaa aatcctgctt tagctggcta
    20881 ccgctgactt gcaaataccc aagatcgctg ctggcggcaa atgaagcatg catatcgtag
    20941 gcattgtcct gactacggct ccatccctgg cgatatccca gacttgcttc ttcagggtgg
    21001 tgatattaat ggctccgccc atctgattag gcccatgaag cagcgacgaa taccctttgg
    21061 aaacttcgac tgcccccaga ttgttggtca gaacccgcgc cagatcgagg ttgccgtcat
    21121 agggaacata aatgggcaca ccgtcgaaat agaccggcac ctgccgacta tcaaagccac
    21181 gaacttttac ctgttcttcg ttgcggctgc ctgacttttg cagcaccacg ccggggacga
    21241 cacttaatgc ctgagcgaca ttctgtttat ccaaggcttt catggtgggt tgatcgagaa
    21301 cggtggtcgt cgtcgatgat accggactgg accatacggt cagcgtgtcg cctcctgagc
    21361 cgctacggcc tgctgggtaa aggcaagtga taatgctgtg cagagataac gttttttgag
    21421 tctcatagaa taaattccgt ttaacaaaga attaaataga ggctgtgggt tgcagactga
    21481 ctttaatgtc agctggtgcc gcgtaataag gcgcggaggt aataaaaagg cgaatgccgc
    21541 agtcgaggta atttttgagt gtcgtcagat taattccgcc ggtcagagcc agcgtgcagt
    21601 gaggagccag cgacggcgct atttgcgcaa tttcagttgc ctgttgcgga ctaaatttgt
    21661 cgagctgaag tacgtctggt tgtgcgcgta acgcggcgat agcctctttc ggcgtgtcgg
    21721 cttcaacaac aattttcttc tcgggtgcgt ggcgacgtaa ctgagcgatt gcgcctgacc
    21781 agccctgatt gtcatgaaga aaatggcggt ggttggcaaa caataatatg gtttccgcac
    21841 atccggcacg atgaattagt cctccggcag ccagaattgc ctgcgaagcc agtaagcggg
    21901 tgcccggaat tgcttttcgg gtgcaggcga tattgccgtc aggataacgt tcacgaagca
    21961 atgccagcat ttgagcgaga taatcagaaa caccgcaact ccactccagc acattctgga
    22021 ctgccttcca tccctgatga agtgctgcgg cattaccctg cgcacggatt agccgttgac
    22081 ccgcgttcgc ttgtgaaccg tcgctgaccg cgtcatcaat agttaatccc agcgtagtta
    22141 acatcttaca cgcgacagaa acgccgctga cgcagccgcc ctgacggtga aaaaacgcta
    22201 tatagccagg ctggtgtcca atatttaatg ctcgcgtggt caggtcacct ccctggatat
    22261 cttccagcag taacgcatcg atttgtgcct gagaaaggaa aatcatcaca gtgcctcctg
    22321 cggtacgcta tcccaccaga tgagcgccca gtcgcggctg gcaggggcga gggcacgagc
    22381 atcttgttgt tgataccagt gatataaccg ctcacgctca tcgtcgttaa gcgcgccaag
    22441 actccagctg acgttctgct caaaacgttc ccaggtgctg ttatcctgtt ggcaattttg
    22501 tccacggata aaatcaacgt gggcataaat tcccatctga tacaggacgt tgagcgcaaa
    22561 gatatagttg ggtaattcga tcacttcccg accagccgcc cgctgaatgg ctggagagac
    22621 gaacgaggtg ctgaccagat gagttgtata gacgcgaagc cgtgcctgat tattcagttt
    22681 gctcattgca tggcgcatat cagccaccag agtggagcgg gaagccacgg caatatcgca
    22741 gcgtggcagg tcactccagt cctcttccca ggcgcgctgg atccagtgga cattgtcagc
    22801 cttcagtgcg gcggcgcggc gggcggcaac gttcagcatc ccctgactgt aatcaacgcc
    22861 ataaaccgta gtgagtttgt cagccagcgc cagcgatacg gtgcctggtc cgcagcccat
    22921 atcgaataag gtctgagcgc cttgcaggtc aattttagcg atcagttgtt gcaggtagct
    22981 gtccgttggc gaggctcaat tttccgccat tttctccgcc cgttgatccc agtgatcggg
    23041 cgttttttct gtgcggtgag ccagtttgag ttgctgcaga taaaggtctg caaaatcaat
    23101 atcatcaatg agcatgattg ttccttagcg gctaattgca gagaggtgat gatgaatttg
    23161 gtcggcagaa acccgataca gggccgccag tttgttcgtt gttaactgtt cggtcggtaa
    23221 gccttgtgtt actcgtccat ctggctctac ctggataatg ctgtcggcaa tggcgttggc
    23281 atggagcgga tggtgagtcg acattaacat tgtcatgccg ttatttttga gctgcgccag
    23341 cgtgtccaga agttggatct gatgaccgaa atcgaggctg gaggcgggct catccagtaa
    23401 caacaagcgc ggttgttgga cgagtgcacg ggcgattaaa gccagctgac gttcaccgcc
    23461 gctaagcgta ttccagcgtc gtgtcgccag atgcaaaatg cccagctttt ccagttgctc
    23521 cgtggctttc aggcgttctt gcttaccggg aacggaaaaa gcgcctatga tgggggccag
    23581 gcccatcaac accatatcca gcacactgaa agcaaaaata ccgtcgtgcg cctgcggcac
    23641 ccaggcaata gcctgtgcgc gctggcgatg gctgagtgtc tgcacaggga ggctatccag
    23701 taggatctcg ccttttaata acggtataac gccaagaatg gagcgcatga gtgtggtttt
    23761 gccgcaacca ttcgcgccaa gcagacaaca aattgttcct tgcgacaggg tgaaggacac
    23821 gtcgcgtagc actggatggc gggagtaacc cagagacgca tgacgaacgg caagcaaagt
    23881 cactggcgac ctccgcgtaa caacaaaagc aggaaaaacg gtgcgccaac aaaagcggtc
    23941 agaatgccaa gtgggatttc tgttgttcca atgctgcgcg ccagcgtatc ggtaagcagg
    24001 agcagaatag cgccagtaca cattgccata ggcaacagct gctgatggtt atgaccggtc
    24061 aacaagcggg caatgtgcgg aacgaccagg ccgatccaac caattatgcc cgctattgct
    24121 accgtgctgg cggtgattag tgtggcgcag acaatcaatc caaagcgcag acgcgtcacg
    24181 ttcagtccca gcgaacgggc ttcgtcgtcg gaaagcgtga gtaaattcat gcgccagcgg
    24241 agaaaaaaca ggggcaagga gcctatgaga atgatcggtg cagcatagca gagatcgcgt
    24301 aaggtcacgg tggacagtcc ccccagcaac cagaaggtaa ttgacggtaa ctgggtgtaa
    24361 ggatcggcca gcgttttaat tagcgatatc cccgctccgc acaacgttcc taacgcaatg
    24421 cctaccagca ccagggttaa gatcggatcg tggcgggtca cccgacgcgt aatcaaccag
    24481 acgcccgcga ccaccatcag ctcgccgcaa aatgcgtaca gctggatgta aacaatgggt
    24541 aatccccaca ggattgccgc gcaggcacca agtccggcac cggcggagac gcctaaaata
    24601 tccggtgaaa ctaagggatt acgcaacatt ccctgatagg tggttccggc tcctgccagc
    24661 gctgcgccca gtaacagcgc agcaagaata cgcggcacgc ggatttgcca gaagacaatt
    24721 tgttcctgca cagacacatt ttcttgacga agaattagcg ccagcagttg ctgcatatcg
    24781 agatgataag ccccacttac ggcggcaaaa gtgatactga tagctgccag cagcaaaata
    24841 catccgttta cgattctcat tagctcgcca ccaatttttg atagtcggca tcactgagtg
    24901 aacaatgcca gaacagttgg gcgtaatgct gcatgtcact tttaaactgg cgattgatgg
    24961 cgggatccag ccaggcgtga agtctgcgta atcccagaag acggttgatt cccggcgggg
    25021 catcgagcca gccaaagggc aagccactta aaaataagat gcgttgttcc gcaacggctt
    25081 tcacgccctg ccagagagga tcacgacgaa taaaatctgc agtaacggcc tcctgaacca
    25141 gaataatatc cggcagccag cgcaggagat tttccatgga aacctgtgtc aggccgtggc
    25201 gatcggctat ttgcgcgacg ttgtgcaaac ctaataattc cgccgcctcg gtatgcaacg
    25261 aaccctgtaa gcccgtttcc agccctcggg gaccgcgcgc agcataaaag cggaggttag
    25321 cggcgggtga ggtggcgaat gcctgagctt caccaacgaa gtggctggcg agattggctt
    25381 gttctgcggc gcggtgctct tcgcctaacg ttttgccaag cgttgttaac tgttctgctg
    25441 attgcgccag ttttccgtta agcaataacc aggggatttg tgtctgttcg ctaacctgcc
    25501 gtgcctggga gatcaaggtt tcatccgtat tgccgcaatc aacgaccaaa tcgggatgta
    25561 acgccataag cccttccagc gatagtgtgc tggcgcgccc ggcgagtcgt cccagcctgg
    25621 gtaactggcg aatgtgctct ggcaatggaa ttaatgcctg acgggcaaaa tcaaaagagg
    25681 aaaaaccgac cattttttct ggcgcgacag ccagcaatag caaatcggcg ggcgcgcctg
    25741 cgctgacgat gcgtgtaatg ttttgggctt cgggaagaga ttgcgaagca aaacgattaa
    25801 ctgtttgtga aaaggacggt agagactgca ggagcagggt agatgcaagt gcctgcgcaa
    25861 acgtccgtct ggttatgggc atggtgaaca tcctgaaaag caaaatcgat atatactttc
    25921 atatatatcg ataaaaatca tgttcaatat caaagtttag ctattgaaaa catgaagatg
    25981 tgacgacttc cgcaagcctg ttgaccctct ctatcattaa aaatgtattt atcaggtggc
    26041 taatattatt taaatcattt aaggctaaat catttaaata tgaatgacat tattgttttt
    26101 tatcgtccac ggttacgcga cgttgaagtt acttgtttta caatgagcga tttttgatat
    26161 ctcgccgaga aaattatcag cattctctcg tttcagtgtc gtgaatatac tgttaacgga
    26221 ggtgaaaaga atgctggggg ttatacaaaa cctatgttgt tgtcgtccgg attcaccgct
    26281 tgctgatagt aagcagcgta accagaagtt accgcaacaa gactttgagg attttaagaa
    26341 cgacgttatt aatgtcatta acaacaatga agatatttat tttcttagag agagtaattt
    26401 actgaatgcg tttattcgaa cgtgcaaaaa aagagatgat aacagttacg gaaatatgat
    26461 atttacgctt tattttgata acgatatcta tgggaacaga attatcaaag catcgctgaa
    26521 cggcggagag ttgcagttat taagcacagg gtgtagcaat gataacattc tccattttat
    26581 taatgcattt ctcattcggg tgaaatttcc gctacctgga cgcataaaac cggaactctc
    26641 gccaaaagcc gggcactccg aaggaattga cgcgttgcgc tgctctgtaa cggcatttac
    26701 cgcaagccat ttatttcgcc agcgacaatc cgcagagaac caaacgcatc aggaaacgac
    26761 tgaacacaat aaatactgta ttgatcaaca tattatactg gcacctccag cacgcagtga
    26821 ggatacactg tatcatcagc gaatcttatc actgcggagc tggttgaacg actttctgag
    26881 cgaaataagt cggttcccgg taacaacaac agaatccttt tttaattatt gtttcgttgt
    26941 ttttggtgtg attcagaaaa taaatatttt agacagtagc cagtcaggcg ttaaaaatgg
    27001 ggcggatagt gaggcggttt tggctgcgct tattgaacat ttagaattga tcaatccttc
    27061 tggcagatta agccaaaata cccgttcggc aatgcttcag ttacgcgaag aggaatggtc
    27121 tgaattcttt ttttggttgc taaattctct ggagtgtctg gattatgtga ttattaattt
    27181 aactccagaa agcaaaaaaa cgttgatgag tgaacaccgt aacaatattc aggtagcaat
    27241 tgatgcttta tatagccaac gtcggcgtaa atcgccgggt gatgaaagtg aaacacttac
    27301 ccgtagaaat gacgctatct tcggcaacca cgtatggcaa acatttgcgc aatacttccc
    27361 gccaggactt gaaaaaccgt ctgtgtagaa aaaaccatag atatggtgtt atctgccgac
    27421 aacgccatcc ggagaagccg aatgacgttg tcggagtaag cgctggttaa ggtgtgggtt
    27481 gtgcctcttt ggttgagggt tgcgtcgttg ctgacttaac ggtcggacgc gtcgccggaa
    27541 cattgtcaca cggttgctct ttcgggcaga tcaaatccag cattttcagc gtcacgccat
    27601 tggtccagcc aaagccatcc tgtaatggat attcgccacc gccgcccccc gttccggtgg
    27661 tgctgacatc atatttttcc accagctttt tctcccggtc ataggtgtgc tgaacattgg
    27721 tcaggaagtg ccagctaatg tccatcgcca cctctttttg cccgtagttt tgtaatcctt
    27781 ctgtcgcgac ccactgtaac ggtgcccagc catttggcgc atcccattgt tgcccacttt
    27841 tcaccgacgt ggtgttcagg ccgccgggtt gcagcagatg tgttttcgtc gccgtcgcca
    27901 ttttgttggc gcgatctttc gctgccgcat tgacgtacag cgggaacagg gcggccgcgg
    27961 ttaactgatt gcgcacttta tgacttttca ggtcgtaatc ggcataccag ccttgttgat
    28021 cgttccacag gtatttttcg atcccttttt gacgggcatt cgccagcgtt tcgtactggt
    28081 ttgccatcgc gttatctcct gcagctttgc tggcgcgggc gaggattttt tccattttaa
    28141 acatcaggct gttcagatcg accggtacga tgctggtggt gcctaaggta tttaactgct
    28201 gcgggttgtc catccagcgc gagctgaaat cccagccaga cgcagcggca gagcgcaggt
    28261 cgcggtaaat ttcagtggca ggtcgattcg gattgctttt ggcggtggca atatcttcca
    28321 cccatgactc tggtcgtggc gtatcgcgat cgtcccagta gcggttgaga agggtaccat
    28381 cctgaagttt tacaacgcgt ttttcctgtt gtccggcttg caggttttca acaccgtcca
    28441 tccagtaagc atattctttt tgcatttgcg gcaggtattg cttcaacgcg gcatcgcctt
    28501 catgctgcgc cagtaactct accatcaggg caaagaaggg cggttgcgag cggcttaaat
    28561 agtaactgcg gttgccgttg ggaatatgac cgtaagtgtc tatttcatga gcaaaattgg
    28621 ccaccatatc cgcgacttta tcccagtgac cgctttcggc aagtcctaac atggtgaagt
    28681 aactgtccca gtaatatacc tcgcgaaaac gtccgcccgg cacgacataa ggttccggca
    28741 gcggtaacag agaatcccat ttttcggtgt tttcggtaga acgcgttaat accggccaaa
    28801 gtccgtcaat atgttcgcgc agtgactgcc cctctggcgg aacatatttc tcgccttctt
    28861 tcggcagggt gaaattgacg ttaacgaaat ggcgcagatc aaatccgctc tggttttgct
    28921 gcatccgata atcagcaagg atcatcagcg gatcgctgtt cggcacggca tcggcaaagg
    28981 ttttttggtc cggaaaaagt ttggcgtttt gcacatcatt aaacagcggc cctaataaaa
    29041 tatcaggcgg ctgtggtgtt accggtgttt cttctgcctg caccgatagc gcagcgaaac
    29101 acaaaaagat acaggctgga attaacgcca ttttttgcgg gcgagaaggt gcgggggatt
    29161 tcatcaatca ttctcctttg gcgaaaccga ataaaacggt attcaagtat cagaaaacct
    29221 tagttcagga tcgcactagc tgcatgatcg aaatctcatt ctgcgaacgg tcaggcattt
    29281 tagaaaaggg aattaaaggg ataaataacg gcgaaataga gtgatcaaat aacgtctgtt
    29341 tattagccag ccagcgacat ttggttcgcg attagcgaag aaaaatcatc ataaattggt
    29401 aaaaaatata acaggtagtt aaaaaacatc agtgctgggt aaattgccgg atgacatcag
    29461 aacgatgcca tccgaacggt ggcttaaccc tgacggttga aacgttgcgt tttaacgtcc
    29521 agcgttagcg tttcttctgg ttgtatcgca tacagtttct caccctgctg gcaaatccag
    29581 ccaatcccca gttcacgggc gattagggcg ctgtgggata ccggacttcc ggcgctaagg
    29641 cagatacctt ttacaaccgc cggatccagt tgcagtactg tggaaggata aatgttctcc
    29701 gccagtagaa tagttggcga gttaaactgc ggga
//
LOCUS       DAETPZ010000044        29418 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.044, whole genome shotgun sequence.
ACCESSION   DAETPZ010000044 DAETPZ010000000
VERSION     DAETPZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.044"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..109)
                     /locus_tag="K0W59_002237"
     CDS             complement(<1..109)
                     /locus_tag="K0W59_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic ABC transporter ATP-binding protein"
                     /protein_id="HBI3023256.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVH"
     gene            complement(179..1168)
                     /gene="araF"
                     /locus_tag="K0W59_002238"
     CDS             complement(179..1168)
                     /gene="araF"
                     /locus_tag="K0W59_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HBI3023257.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAH
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVETPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1965..2468
                     /locus_tag="K0W59_002239"
     CDS             1965..2468
                     /locus_tag="K0W59_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HBI3023258.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(2548..2799)
                     /locus_tag="K0W59_002240"
     CDS             complement(2548..2799)
                     /locus_tag="K0W59_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HBI3023259.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(2914..3000)
                     /gene="azuC"
                     /locus_tag="K0W59_002241"
     CDS             complement(2914..3000)
                     /gene="azuC"
                     /locus_tag="K0W59_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HBI3023260.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            3263..3586
                     /locus_tag="K0W59_002242"
     CDS             3263..3586
                     /locus_tag="K0W59_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HBI3023261.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            3757..4254
                     /gene="ftnA"
                     /locus_tag="K0W59_002243"
     CDS             3757..4254
                     /gene="ftnA"
                     /locus_tag="K0W59_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HBI3023262.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(4291..4530)
                     /locus_tag="K0W59_002244"
     CDS             complement(4291..4530)
                     /locus_tag="K0W59_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HBI3023263.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            4720..5931
                     /gene="tyrP"
                     /locus_tag="K0W59_002245"
     CDS             4720..5931
                     /gene="tyrP"
                     /locus_tag="K0W59_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HBI3023264.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVPSPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(5993..6658)
                     /gene="yecA"
                     /locus_tag="K0W59_002246"
     CDS             complement(5993..6658)
                     /gene="yecA"
                     /locus_tag="K0W59_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HBI3023265.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(6854..6940)
                     /locus_tag="K0W59_002247"
     tRNA            complement(6854..6940)
                     /locus_tag="K0W59_002247"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6904..6906),aa:Leu,seq:taa)
     gene            complement(6953..7026)
                     /locus_tag="K0W59_002248"
     tRNA            complement(6953..7026)
                     /locus_tag="K0W59_002248"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6992..6994),aa:Cys,seq:gca)
     gene            complement(7308..7856)
                     /gene="pgsA"
                     /locus_tag="K0W59_002249"
     CDS             complement(7308..7856)
                     /gene="pgsA"
                     /locus_tag="K0W59_002249"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBI3023266.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(7913..9745)
                     /gene="uvrC"
                     /locus_tag="K0W59_002250"
     CDS             complement(7913..9745)
                     /gene="uvrC"
                     /locus_tag="K0W59_002250"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBI3023267.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(9742..10398)
                     /gene="uvrY"
                     /locus_tag="K0W59_002251"
     CDS             complement(9742..10398)
                     /gene="uvrY"
                     /locus_tag="K0W59_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HBI3023268.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            10694..10870
                     /locus_tag="K0W59_002252"
     CDS             10694..10870
                     /locus_tag="K0W59_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023269.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            10857..11081
                     /locus_tag="K0W59_002253"
     CDS             10857..11081
                     /locus_tag="K0W59_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HBI3023270.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(11114..11734)
                     /gene="sdiA"
                     /locus_tag="K0W59_002254"
                     /pseudo
     CDS             complement(11114..11734)
                     /gene="sdiA"
                     /locus_tag="K0W59_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(11964..12716)
                     /gene="yecC"
                     /locus_tag="K0W59_002255"
     CDS             complement(11964..12716)
                     /gene="yecC"
                     /locus_tag="K0W59_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HBI3023271.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(12713..13381)
                     /gene="tcyL"
                     /locus_tag="K0W59_002256"
     CDS             complement(12713..13381)
                     /gene="tcyL"
                     /locus_tag="K0W59_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HBI3023272.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(13396..14382)
                     /gene="dcyD"
                     /locus_tag="K0W59_002257"
     CDS             complement(13396..14382)
                     /gene="dcyD"
                     /locus_tag="K0W59_002257"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HBI3023273.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(14487..15287)
                     /gene="tcyJ"
                     /locus_tag="K0W59_002258"
     CDS             complement(14487..15287)
                     /gene="tcyJ"
                     /locus_tag="K0W59_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI3023274.1"
                     /translation="MKLAHLGRQALMGVMGVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(15375..15926)
                     /gene="fliZ"
                     /locus_tag="K0W59_002259"
     CDS             complement(15375..15926)
                     /gene="fliZ"
                     /locus_tag="K0W59_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HBI3023275.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(15972..16691)
                     /gene="fliA"
                     /locus_tag="K0W59_002260"
     CDS             complement(15972..16691)
                     /gene="fliA"
                     /locus_tag="K0W59_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HBI3023276.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDAPQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(16856..18508)
                     /gene="fliC"
                     /locus_tag="K0W59_002261"
     CDS             complement(16856..18508)
                     /gene="fliC"
                     /locus_tag="K0W59_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HBI3023277.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIILQAGNSVLAKANQVPQQVLSLLQG"
     gene            18774..20180
                     /gene="fliD"
                     /locus_tag="K0W59_002262"
     CDS             18774..20180
                     /gene="fliD"
                     /locus_tag="K0W59_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBI3023278.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            20205..20615
                     /gene="fliS"
                     /locus_tag="K0W59_002263"
     CDS             20205..20615
                     /gene="fliS"
                     /locus_tag="K0W59_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBI3023279.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            20615..20980
                     /gene="fliT"
                     /locus_tag="K0W59_002264"
     CDS             20615..20980
                     /gene="fliT"
                     /locus_tag="K0W59_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HBI3023280.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            21058..22545
                     /gene="amyA"
                     /locus_tag="K0W59_002265"
     CDS             21058..22545
                     /gene="amyA"
                     /locus_tag="K0W59_002265"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBI3023281.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(22579..22992)
                     /locus_tag="K0W59_002266"
     CDS             complement(22579..22992)
                     /locus_tag="K0W59_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBI3023282.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            23179..24384
                     /gene="yedE"
                     /locus_tag="K0W59_002267"
     CDS             23179..24384
                     /gene="yedE"
                     /locus_tag="K0W59_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HBI3023283.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            24381..24614
                     /gene="yedF"
                     /locus_tag="K0W59_002268"
     CDS             24381..24614
                     /gene="yedF"
                     /locus_tag="K0W59_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBI3023284.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            24721..25391
                     /locus_tag="K0W59_002269"
                     /pseudo
     CDS             24721..25391
                     /locus_tag="K0W59_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(25431..26639)
                     /locus_tag="K0W59_002270"
                     /pseudo
     CDS             complement(25431..26639)
                     /locus_tag="K0W59_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            26707..27322
                     /gene="tnpA"
                     /locus_tag="K0W59_002271"
                     /pseudo
     CDS             26707..27322
                     /gene="tnpA"
                     /locus_tag="K0W59_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            27650..28732
                     /locus_tag="K0W59_002272"
     CDS             27650..28732
                     /locus_tag="K0W59_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HBI3023285.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(29221..>29418)
                     /locus_tag="K0W59_002273"
     CDS             complement(29221..>29418)
                     /locus_tag="K0W59_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3023286.1"
                     /translation="SEGISFQSILDDVRLNNALSAIQTTVKPISEIARENGYKCPSRF
                     TERFHNRFKITPRELRKASRE"
BASE COUNT         7416 a         7406 c         6888 g         7708 t
ORIGIN      
        1 catgaacctg accggcatag cagtcaaaac taatatccgt cagcgcctta acgccgggaa
       61 acgttttacc gatgccgcga aatgagagat acggggtaga ctgttgcata acgtctccgt
      121 gaatcactgg tcgttcacac cccctctgca tcatgcagag gggaattttt ccggcaaatt
      181 acttaccgcc taaacctttt ttctccagtt cttctttaaa gttgtcacgc gtgatcagta
      241 ccacgtctgt aacttcggta aattttggcg tttcaacgtc ttttgctacc cagttgtaaa
      301 gcatttcgct ggatttatag ccatgtacgt ccgggcttgg cagcagggaa ccgtagaagc
      361 cggttgcctg tgctttagac agttcgctta ctgcatccac accgttaata ccaataccga
      421 tgatatcggc tgctttaaag ccctgacctt cggttgcacg tacgccgcca agtacggtgc
      481 tgtcgttcat accgacgatc agccaatgtt taacttccgg atgttgaacc agcattgagt
      541 tggcagcgtc aaatgccccc gggatgtcgt tagatttggt aggtacctga taaatttgtt
      601 tttccgggaa tcctgcagct ttcagtgcgt ccatagaacc cgttgtacgg cggcgggcgg
      661 tatccagttc gttggcggta atcgccatca ctgcgctttc tttgacatcc cagccacgtt
      721 tctgcatctc tttatacagt tcctggccct gacgttcgcc aattttagtc gccgccatca
      781 tcaccagagg aacggtatcc attggcttac ctttggcgtt aacaaactgg tcatccactg
      841 caatgacttt catatcgtag ccatgcgctt tcgcgacgat ggcagagccg agtttcgggt
      901 ccggagtaca aataacgaaa ccttttgcgc cactggctgc caggctgtcg atcgcgttca
      961 atgttttttc gccatccggc acggcaatct taataacctc aaaccctaaa tccttcccgg
     1021 ctttatcggc aaacttccat tcggtctgga accacggctc ttccggttgc ttcaccagaa
     1081 aaccgagctt caggttctcc gccatagcgg attgtgacat aacggctgcc agaccaatgg
     1141 ctgccagggc tttagtaaat ttgtgcatgg ttctctccag ctttagtgtc gttttgtgta
     1201 gggcaaaaac gaatgacatt cgttaaatta atcggaaaac aaagcattac cttttatcta
     1261 aaagataagt gaccgtgttg acatagtttt agcgagaaat taattctcca taggagagca
     1321 atatcacatc gcagaattac agtgagaacg tgcataaatt tagcgggaaa agacataagg
     1381 aaaagccaat ttgtcagaca aattgttgaa tgcacagcag attaatccat aagattagcc
     1441 tggaaatcct tgttgtcttc ggtacccatg cgggatgtct tctttttaac cagtcaatag
     1501 gccgcattac ctggcgttga gtttttgaaa tggtgtaata accacaactc aaagatgtgg
     1561 aaaatgcgcg tcattcattt cgccattaat tatcactgtg ctcattaatt aacagaacac
     1621 gtataatgag agccatctcg caaaaatgaa aaaacgtttt ataaaatcat cacttcatca
     1681 tgaattcaaa ttcatcgatt aatatcaaca agatacaaaa agcactatca ttaaaattca
     1741 ttgcatttac attgatttca ccaatgaaat gtaaaaatat ataaacttga tgatttaagc
     1801 attttcttat aaccgttcag acgttattct tatttcagat catcgtcaga attgactcca
     1861 cgatcacatt tcggaccggc agaaaggaat tattctgcaa acagtaatta tggtgttttg
     1921 atttatcttg cacctctcca cttctggata taaggatatt aggtatggca accgctggaa
     1981 tgcttctcaa actcaactct caaatgaacc gcgagtttta cgcatccaat ctctaccttc
     2041 acctgagtaa ctggtgttct gaacagagtc tgaacggcac cgccactttc cttcgcgccc
     2101 aggcacagag taatgtgacc caaatgatgc gcatgtttaa ctttatgaag agtgtcggcg
     2161 ctacccccat cgttaaagcc attgatgttc caggtgaaaa actgaactct ctggaagaac
     2221 tgttccaaaa aacgatggaa gaatacgagc aacgttcaag cacgctggcg cagttagccg
     2281 atgaagcgaa agaactgaat gatgattcaa ccgtcaattt cctgcgcgat ctggaaaaag
     2341 aacagcagca tgatggcctg ttgctgcaaa ccattcttga tgaagtgcgc agtgcgaaac
     2401 ttgcgggtat gtgccctgtg cagaccgacc aacatgttct gaatgtcgtg tcacaccagc
     2461 tgcattgatc atacatcggc gctaatacat tgcgccgatg aagtttttga gaaaccgctg
     2521 cctcatctgt ttgaagcagc ggttttttta atgggattca ccctgtgggg taaacttgag
     2581 ttcaatcagc gcgatggctt tttggattgc ccgcatggtg accgggtctg cggcagcggg
     2641 atgatgagta aagtcgatat tcttcagctg actggacatt ttttcacgaa cttcaacggg
     2701 cgcgattaca tcgagaacat ccagaatttg tttgataacc aactggcaag ctaccacatc
     2761 agaaaccagt tcctgatcgg cattcagcgg ctgggacatc gtaaactcct gatagcattt
     2821 tgaaagccgt tatagtagcg gcttcacatc ttcagcgata gtcacatcca ccgtcatcag
     2881 gacacaaaaa aacctgccgg agcaggtttt ttgttatcgg aacatattgc ctggcggtac
     2941 gtctttgaac gtcttgcaat agttattgaa catacttttc aggattttgc gcagtttcat
     3001 cgcggcactc cgaccatttg ttatacaggt gttattgtct ttgcgcttat aatatgacaa
     3061 ccatcacaaa aatcaatctt tatgtgatac aaatcacata aatacccctt taatgttata
     3121 aaaatgataa tcaaaaaaca gcccccctat ttctgacacc tacagatggc aagaaatagc
     3181 gcctgccagg catcttttcc ggccattgtc gcagcgctgt aacgcgtaaa atagtgcttt
     3241 ctcttactct tctggctgga ccatgagact tctgattctg actctttcat taataacgct
     3301 ggcggggtgt acggttactc gccaggccca tgtgagcgaa gttgacgcgg caacaggtat
     3361 tgtacggttg gtttatgacc aggctttttt gcagcatgcc cataccgatc gttatgtcag
     3421 tcgcgggatc gccgatcgcg catgccagca ggaaggctat acccacgcga tcccctttgg
     3481 tcagccagta ggcaactgta gcctttttgc gggttcctta tgtttgaata cggaattcac
     3541 tttgtcatat cagtgccatc attccgcctt ccctgtcttt ctctaaatat tttaatcagc
     3601 gaggggatct tcgctgatta aagaaatagc ggtaatgcgt tttattccca ttcgtatttt
     3661 taataattga agtttatatt ttaccttttg caaataataa aataacaaat tatagtgacg
     3721 ccacagcaac aaatataacc tttgtggagc actatcatgc tgaaaccaga aatgattgaa
     3781 aaacttaatg agcagatgaa cctggaactg tactcttcac tgctttatca gcaaatgagc
     3841 gcctggtgca gctatcatac cttcgaaggt gctgccgcgt tcctgcgccg tcacgcccag
     3901 gaagagatga cgcatatgca gcgtctgttt gattacctga ctgataccgg caatttaccg
     3961 cgtattaata ccgttgaatc tccgtttgct gaatattcct cacttgatga attattccag
     4021 gaaacctata aacacgaaca attaatcacc cagaaaatta acgaactggc tcatgctgca
     4081 atgaccaatc aggactaccc aacatttaat ttcctgcagt ggtatgtttc tgagcagcat
     4141 gaagaagaga aactgttcaa atcgattatt gataaattaa gcctggcagg caaaagcggc
     4201 gaaggtctgt attttatcga caaagaactc tctaccctcg acacacaaaa ctaatgctct
     4261 ccggcggcag gtttctgccg ccgtttcaaa tcagtgacgg caaatcttac tctgatcggt
     4321 cgaaaaacct tcttctgaag gtataaattt gccttttgct gccagaaacg ctaccagctc
     4381 tcccgctgtc atcccttctg ccgagcacgt gtgaaaacgt gcctgttcac caaaacgcgc
     4441 tttaatcgca gcttccagac tggcatgcgt atattgctcg cctgattcaa tcatcatatt
     4501 taacacttca tgaccgtgaa tagagtccat cgttcctcct caaaaaaagc ctagcgtagc
     4561 gattaccgct tatgaagact ttgcgccagc gcaggactga atgcttttat tgtacattta
     4621 tatttacaca atatgtaacg tcggtttgac gaagcagccg ttatgcctta acctgcgccg
     4681 cagatatcac tcataaagat cgtcaggaca gaagaaagcg tgaaaaacag aaccctggga
     4741 agtgttttta tcgtggcggg aaccacaatt ggcgcaggca tgctggcaat gccgctggct
     4801 gcggccggtg ttggttttag cgttacgtta atcttgttga ttgggctttg ggcgttgatg
     4861 tgctacacgg cgctattact gctggaggtg taccagcatg ttccggcaga taccggtctg
     4921 ggcacgctgg caaaacgcta tctgggacgc tacggtcaat ggctgacggg cttcagtatg
     4981 atgttcttaa tgtatgctct gactgcagca tacatcagcg gtgccggtga attgttggcc
     5041 tccagcatca gcgactggac aggtatttca atgtcggcaa ccgctggcgt gctgttgttc
     5101 acttttgttg ccggtggcgt ggtttgtgtt ggaacatcgc tggtcgattt atttaaccgt
     5161 tttctgttca gcgccaaaat tatttttctg gtggtgatgc tggtattgct gctaccgcat
     5221 attcacaaag tgaatctttt aaccctgccg ttgcaacagg ggctggcact gtctgcaatc
     5281 ccggtgattt ttacgtcgtt tggttttcac ggtagcgtgc cgagtattgt cagctatatg
     5341 gatggcaaca ttcgtaagct acgctgggtg tttataatcg gtagtgccat ccccctggtg
     5401 gcatatattt tctggcaggt ggcgacgctt ggcagcattg attcaacaac ctttatggga
     5461 ttgctggcta atcatgctgg attaaacggg ctgttacagg cgttacgcga aatggtgccc
     5521 tctccgcatg ttgagctggc ggtgcattta tttgctgatt tagccctcgc cacgtcattt
     5581 ctcggcgttg cgttaggctt atttgattat ctggctgatt tgtttcagcg ttcaaatacc
     5641 gttggtggac gattgcaaac tggtgcaatt acctttctgc cgccgttggc gtttgcactg
     5701 ttttatccac gaggatttgt gatggcgctg ggttacgccg gtgtggcgct ggcggtactg
     5761 gcattgatta tcccttcact attgacctgg caaagcagaa agcacaatcc tcaggcgggt
     5821 taccgggtca aaggtggtcg tccggcgctg gtggtggtgt ttctctgtgg tattgctgtg
     5881 attggcgtgc aatttttgat tgcggcaggg ttgttaccag aagtggggtg atcagatagc
     5941 ctcaaattcc ttattgggtg ccagaattaa cgctgacacc caatgtgacc tcttaatgca
     6001 ggcagcactg cttaaatttc ttaccactgc cgcacggaca aggatcgtta cgccccggtt
     6061 tctcttctgc tttgatcggt tgctgaacag ctttttcctg cggatgcgcc atccagtatg
     6121 catgtaaatc aagcgccgcc agtcgaatgg catctacgct ctcttcaaac gcttctggcg
     6181 acattttctc tacccgctcg aagttttcct cagtaccgtg cagcgcaatc gcctccagcg
     6241 ctggttttaa agaatcgggc aacgttgacc agtcagaaag tgccacgccc cgcatatagc
     6301 caaagcacca ctcctcaaca atcgtcagct cgctgccatc aacttctcgc aagccgaata
     6361 acggctcaaa ctgctccggg aattcgttca gacgctctgc ggtatcggcc atatgttgaa
     6421 aagccaggtt cataaagcgc gtcatctctt tctctgacgc ccaacgcggc acatagtcag
     6481 ccccacccca cacggcaacc agccactgtt ctggttcaat ctcttgcgga gaactcaaca
     6541 ccgccgtcaa taaaccgtcc agctccgcca catcaaggat ggcgtggtca gtgttgtatt
     6601 tggtcagaat atcgtccagc cattccaact cactttcgtt taacggtccc gttttcatac
     6661 gcttttcctt gtggatctca actcgccagc acctatctta catgccggtc cgtatcagag
     6721 atactttttg agtggctttg ccggtgatta aaaattaagg agggtgtaac gacaagttgc
     6781 aggcacaaaa aaaccacccg aaggtggttt cacgacactg cttattactt tgattttatt
     6841 cttatctttc ccatggtacc cggagcggga cttgaacccg cacagcgcga acgccgaggg
     6901 attttaaatc ccttgtgtct accgattcca ccatccgggc tcgggaagaa agtggaggcg
     6961 cgttccggag tcgaaccgga ctagacggat ttgcaatccg ctacataacc gctttgttaa
     7021 cgcgccaaat tcttcaggcc tttcagccag acatccgctt gacgccgatg tcttttaaac
     7081 tggagcggga aacgagactc gaactcgaga ccccgacctt ggcaaggtca tgctctacca
     7141 actgagctat tcccgtattc atcaagcaat cagttaatca cttgatttta ttatcgtctg
     7201 gcaatcagtg ccgccgttcg atgcgttgca ttctacttac ctggcgcgat gagtcaacga
     7261 tatttttcac cacttttgat cgtttgctga aaattacgcc gaaacgatca ctgatcaagc
     7321 aaatctgcac gcgcagcgct caaatattgc aacattgacc acagagtcag taccgcagcc
     7381 acaaagaaaa gtgcaatacc ggcgtactca acccaaatgt tcggacgcca cagcagccag
     7441 gccaacgcca ccatctgggc agtggttttc actttcccaa tccaggagac ggccacgcta
     7501 ctgcgtttac ccaactccgc catccattcg cgtagcgcag aaataataat ttcacgggcg
     7561 atcatcgttg ccgccggtaa tgtcacccac cagctgtgat aatgctcggt taccagcacc
     7621 atggcgatag ccacgagaac tttatctgcc acagggtcaa ggaaagcacc aaaccgggta
     7681 ctctggttcc agcggcgtgc cagaaaacca tcgaaccagt cagtcaccgc cgcgacgcag
     7741 aaaatgagcg cggcggcaaa cggcgaccag gtgacaggca gataaaagac caatacaaag
     7801 aatgggataa ggatgacacg gaacagtgta agcaacgtag ggatattaaa ttgcataatg
     7861 acgggtaact atctgttgtc agtaagatta cccctatgtt gctacagaga catcaatgtt
     7921 tcaacgacca gaagatcttt tctgccagac cttgcgaaat acccggcact tttgcaattt
     7981 cctcgacgct ggcgttacgt aaaccttgca aaccgcccat atatttcaac aacatttgcc
     8041 gacgttttgg cccgacgcct tcaatggttt ccagggaact ggtatttttg accttcgccc
     8101 gttttttacg gtgcccgcca atcgcgtgat catgtgattc atcgcgaata tgctggataa
     8161 catgcagcgc gggtgaatct ggcggcaaac taaatccctc accttccggc tcaaagaaca
     8221 gcgtttccag tccagcctta cgatctgctc ctttggcaac gccaagtagc agcggatgat
     8281 ttttatccca tgagacatcc agttcggcga agacattttt cgcctgcgca agctggcctt
     8341 tgccgccgtc gataaggatc acatccggga tcttactgtc gtcaatggct ttaccataac
     8401 gccgacgcag cacctgattc atcgccgcat aatcatcgcc cggcgtgatg cctgtaatgt
     8461 tatagcgccg atactccgca cgcagcgggc cgttagcatc aaacaccaca caggaagcga
     8521 cggtttgttc gcccatggta tggctgatgt caaagcactc catccgcttc acttccggca
     8581 atttcaacac gctggcaagc gcggtcagtc gctggtgaac ggtagattgc tgcgaaagtt
     8641 tgctggttaa ggccgtcgcc gcattggtgc gcgcgagttt cagataacgc gccctatcgc
     8701 cgcgaggttt ggtttgaaca ttaatcttgc gtcccgccag ttctgaaagg gaatcggcga
     8761 gcagcgtttt atcgctaaga ttaaaatcga gcaggatctc acccggtaag gtgcgcatct
     8821 ggctgccttg taaatagaac tgacctacga aggtttctac cacctcgctc agttccgtac
     8881 cgccaggcac tttcgggaaa tagctgcggc tgccgagcac tttgccctga cgaatgaaca
     8941 atacgtggac acaagccatg cccgcatcga acgccacacc aataacgtca aggtcgtcgc
     9001 cggtattgga aacaaactgt ttttcggtga cgcgtcgcac cgcctgaatt tggtcgcgaa
     9061 tacatgccgc ttcttcaaac tccagattct ggctggcagt ttccatacgg ctaatgagtt
     9121 gcgtaagcac ctgatcatct ttgccagaca aaaacaggcg cacatactcg acctgctgag
     9181 cgtattcttc ttcactcacc agtccttcaa cgcacggtcc cagacagcgc cctatctggt
     9241 attgcagaca cggacgcgag cgattgcgat aaacactatt ttcgcactgg cgaatgggga
     9301 aaatcttttg cagtagcgcc agtgtttcac gtacggcata gccattcggg aacgggccga
     9361 aatattcacc tttggcatgc tttgcaccac gatgcatcgc cagacgcggg tgggtatcgc
     9421 cactcaggaa gataaaagga taagatttat catcgcgtag caaaacgttg taacgcggct
     9481 gatagagttt gatgtagttg tgttccagca acagcgcttc ggtttctgta tgcgtaaccg
     9541 ttacatcaat ttgctggatc tgggcgacca gcgcttcggt tttgcgcgaa gcgaggttgc
     9601 tgcggaaata gctggaaagc cgttttttca ggtctttcgc tttgccgaca tagataaccg
     9661 taccaccagc atcgtacatg cgataaacgc ctggctggct ggttacggtt tttaaaaacg
     9721 cttttgcgtc aaactgatca ctcactgact tgataatgtc tccgcattac acagaccatg
     9781 gcgaattgcc aggtgagtca gctcaacatc gccatgaatg tttagtttac tgaacatacg
     9841 atagcggtag ctgttcaccg ttttcggact gagattgagc tgttctgaga tctcattgac
     9901 cttctggccc ttagtgatca tcagcataat ctgcaattca cgttcagaca aactggcaaa
     9961 tgggctttct gttttttctg gttcgatctg gcttaacgcc atttgttgag cgatatcaga
    10021 agcaatgtaa cgctgccctg aatagacaga acgaatcgca ctcacgactt cctgcggagc
    10081 cgcgcctttg ctgaggtagc cagcagcacc tgcctgcatg actttcgctg gtaaagggtt
    10141 ttctgtatgg accgtaagca tgatgatttt aacatcagct gtggaacgcg cgattttacg
    10201 cgtcgcctca agaccgccaa tgcccggcat actcatgtcc attagcacca cgtcaacggc
    10261 atttgtccgg caccacttaa cggcgtcttc accgcacgat gcctctccga cgacttttat
    10321 accctttata tcttccagaa tgcgtcgtat ccctgcgcgc accagttcgt ggtcatcaac
    10381 aagtagaacg ttgatcaaag gaatatctcc agaaataggg ataacgctac tgatagttag
    10441 tcattcgtat attagcggtt tttattgcaa ctttgaaacg ttaaaaatgg tgggtattcg
    10501 atttttctct cgtttttgtc aattcgattg tccacagtta tggtcacgcc cgtcaccaac
    10561 ggcttaccgg atcgtttcac agtaagccct acgatgaaat gtgacaaaaa ttgactttat
    10621 tcagcaaaaa tgaaaatcag cccgctgaat atgtaacatt aattaacccg aacaatacgg
    10681 gcaaaaaaca ttcatgataa aaatatttat tggtcattat attaacgtat tttatagcac
    10741 tgccgatatc acgctcaaaa aacaaccact gctattttta gcaaagctta tggtatactc
    10801 cgccgcctta acatttttca ccgcaaattt tcattgcaac atgacgagga aaataaatga
    10861 gtacgcctga tttttctact gccgagaata atcaagaact ggcaaatgaa gtctcctgcc
    10921 tgaaagcgat gctgacgctg atgctgcagg cgatgggaca agctgacgcg ggccgcgtga
    10981 tgttaaagat ggaaaaacag cttgcgctga tcgaagacga aacccaggct gcagtatttt
    11041 ccaaaacggt taagcaaatt aaacaagcct accgtcagta atataaaacc ggctgagagc
    11101 gtgcctttca gccggttatt gacaaaatca tcgctatctc tgctgatgtc ttcccttccg
    11161 ccgtccattt cagaatttct ttttcgcgct tgctgaaatt catctctggc gtcatcacta
    11221 tttcatcatt taaacgcatc agagccatca gactttcgcg caccagtaac tgcattttta
    11281 attgcagttc atcactaaga atgggtattt cgcgcgtgct gcaacgggaa aaggacaaaa
    11341 agcccagcgc ccgatttggc agcattaaat actgagtgac accgcggcgt aaaccatgtg
    11401 cgtgcgcggc ttcccataat ggctgtgctt cgctgaataa gtcatcattc cacattaaat
    11461 ggtcctgact aaagttttca gggttcagca caggatcaat tgcgagaaaa ttttttgcct
    11521 gataataact aacccacgac tcagggtaat tggtgtagaa agccacttta ggtcgcgtga
    11581 atggtaccgg gtggcgaaca cataacgaat agtaatcgta ctccagctgc tgagcctgaa
    11641 gctctatttc atggtagacc tcttctgcgg cctccatcct ctgaaaacgc aacagcatcg
    11701 tgcgacgcca gctgaaaaaa tccgtatcct gcatagtaaa ccgcaacgcc cctgagagtg
    11761 agtatcattt ataatgataa ttcaaactat cacataaata agatttatat ataatttata
    11821 ttattcaggc aatgaattac ttttgcaagc catcgcattc tcttatgtta ttaatgagtt
    11881 atgctgattt gttaagcagt tttatcacgc ttgaaatggc gtcccgcccc gacaggtgaa
    11941 tcgtcggggc tgattttttc ttattattgc agcagaagct tctcgaggaa ctggcgggtg
    12001 cgaggctgct ggggatcggc aaataacgct tttgctgccc cctgctcgac tatccgcccc
    12061 tggtccataa agatcgcccg gtccgcaaca tcccgggcaa agctcatttc gtgtgtcaca
    12121 atcaccatcg tgcgcttttc ctgcgccagc tggcggatgg tgttcaggac ttcgcccacc
    12181 agctctggat ccagcgccga cgttggctcg tcaaacaaaa tcacctcagg acgcattgcc
    12241 agtgcacgcg caatcgcaac acgctgctgt tgacctccag acaaacgacg tggatagctg
    12301 gtttctttac cggccagccc aactttcgcc agcagctcgc gagcgcgcgc cgtggcctct
    12361 tctttcggtt cgcctttgac gatcaccggc ccttcaataa tgttttccag caccgtacga
    12421 tgcggaaaca aattaaagtt ctggaagaca aacccgacgt gctgacgcaa ctggcgaatc
    12481 agcgattttt gctgacttaa tgaacgtgca gtatcaatag tgatatcgcc aacggtgatc
    12541 gttcccgctt ccggttgttc cagcagattt atgctacgta gcaatgtggt tttgccggaa
    12601 ccactcggac cgataattgc caccacttcg ccaggcttta cctcgaggtc gataccgtgc
    12661 agcaccgtct gaccgtggaa ttttttcacc aggttcttaa cttcaatggc actcattttg
    12721 gttctctctc ctggcgatta agttgattct caaaatggtt ctgcaacgtc gataacacag
    12781 tcgccatgat ccagtagatg agcgaagccg ccaggtacat ggtgaacacc tccagcgtgc
    12841 gcgaggtaat caactgcgcc tgacggaaca gctccggcac ctggattgtc gcggccagcg
    12901 acgtatcttt taccagactg ataaagctgt tcgacagcgg cggcaacgcc acccgcgccg
    12961 cctgtggcaa aatcgcgcga cgcatggtct gccacggcgt cataccaata ctggcagcgg
    13021 cttcccactg acctttatca atagaagaaa tggcggcgcg cagcgtttcg gcggcatagg
    13081 cggcagtgtt cagcgacaga ccaatcattg ctgacggaat gggatctaat tcgataccaa
    13141 actgcggcag gccgtaatag atcataaaca gttgtgcgat gagcggcgta ccacgaaaaa
    13201 tagagatata aaaacgcgcc agccagcgca cgggccagat tggcgacagg cgcatcagcg
    13261 cgagaataaa cccgagtagt aagccaaaaa acatgccgcc gatactgagt tgcagggtat
    13321 acccagcccc tttcaacagg aacggcaagg aatcaataac cagttgtata ctttcttgca
    13381 tgagcgttcc cggagttaaa cgtggggatg ataggcgaac agcgcaggcg cgccgccggt
    13441 atgaataaac agaatcggcc cttcatcttt gaagcgtttc tgactgatac cgtcaatcag
    13501 ccccgccatc gcttttccgg tatacacagg atcaagcaga ataccttcca gacgcgccag
    13561 caatttcact gcttccatgc cttcgtcgtt cggcacgccg tagccaggtg caaaatagtc
    13621 atcccagagt aaaatttccg ctgatgcggt cagctccagt tctttcgcaa tcgcctgttg
    13681 taggttaacc actttcggca attgatcggc aacggaacgc gacacggtca cgccaatcag
    13741 ttcgctttca ggcatcaggt gttccagccc aacagccagt ccggcgtgag ttccggcact
    13801 gcccgatgcg actaccaccg acgaaatatt aaccgcccct tcacactgtt gcgcgatttc
    13861 cagcgcactc tccacataac ctagcgcgcc aagagcatta gaaccgccaa ccggaatgac
    13921 atacgggcga aagccttgtg cttcgactcg cgtcgccagc tcttccagtt gggcattggg
    13981 atcggtcagt gcgtcgcaca tttcaatctg ggtattgaac agatccagca acaaacgatt
    14041 gccgttggtt aaatagtttt ctgcggttgt gccaatagga ttttccagca gcgccacgca
    14101 gtgcagaccg agtttcgccg cgactgcggc agtctggcgc acatggttag actggatcgc
    14161 gccggcagta atcagcgtat cggcaccttc gcgcagggca tctgccgcga gaaattccag
    14221 cttacgtaat ttattgccgc ccattgccat gggggtgacc tcatcccgtt tgatgaaaat
    14281 ttcccgtcca agataatcag aaaagcgcgg cagatattcg agcggcgttg gcgcgccgat
    14341 aaactccaac cgtggaaaac gggttaaatt atgcagtggc ataacagcct ccgatgtgtg
    14401 ttgttgtgat tttcttatta tgcacgctga aaacgcgtaa ataaaaaagg cgctagtgaa
    14461 agcgcccttt tttgtcatta tgctgattat ttggtcacat cagcaccaaa ccatttttcg
    14521 gaaagagctt gcagagtgcc gtctttttgc atttccgcaa ttgcatcatt caccgctttc
    14581 agcaggtctt catttccttt acgcagcgcc acgccagact cctgacggga gaatgcttca
    14641 ccggttactg ccagcgtatc gttggttttc ttcaccagat ccagcgccgc cagacgatca
    14701 acgaggatcg catcgatacg ccctacgcgc agatcctgat atttggtcgg gtcatcatca
    14761 taggtacgca catcgacacc ctgaacattc tgccgcagcc actcttcata gttggtgccc
    14821 agaccgacac ccactttttt gcctttcaga tcagcagccg ttttaatggt gccttcgtta
    14881 ccttttttca ccagcgcctg aataccggaa atggtgtaag gggtagagaa atcgtatttt
    14941 ttcttgcgct cgtcagaaat ggtgacctga ttaatcacca catcaatgcg tttagagtcc
    15001 agcgacgcca gcataccgtc ccatttggtc ggttttagtg acgcctcaac gccaagatgt
    15061 tttgccagct gttgggcaaa ttccacttca aaaccggtta atttgccgtc atccccctga
    15121 aaactgaacg gcggataagt gccttccagc cctaccagca gcgtgccgcg ctctttgact
    15181 ttattaagca gaccttcatc tgcaaaactt ttaacgctca tgcccgcaac cagcgccacg
    15241 cccatcacac ccatcaatgc ctgacgtccc agatgtgcta atttcatatt caccccgaat
    15301 gttgttatgt ctgtttgcag tgtagagcca ttgtttgtaa acacaaaaac aactccgcta
    15361 catcttattc ttatttaata tatatcagaa gcaggcaggc tggaggattt ctgcacaagt
    15421 ccggtacagg tttggcgctg atagtgttga tacttccgta atgcgatgcg gtaattgttg
    15481 gtgctggtgg tgggcagcca cggggcgagg atttcatcaa ggaaaccgtc ctgcagctga
    15541 tcgagggaaa tattttgctc atgtagatga ttacccaaac ggcgcagacg aacgacatat
    15601 tcacgcaccg tacctgggct catttcagtt tgctcaaaaa gaaatttctt aaagccgata
    15661 atatcgaaaa aatcactctg cgttttgcaa tgtaaatcac cgcaaaatcg gcacaatgcc
    15721 gcccatgatt gttgttccgt ttgccagcct ttttcatcaa gcagcgtgtc caggcgggaa
    15781 atctcgattt tattcactat tttgccgtcg cgaactaagg taatgcgatc gagtaattta
    15841 cggcaatgcg cgcaatgggt ctggctgtgt ttaaagtctt taagatagcg gcttaatggc
    15901 cgtcttttca ggtgctgcac catcattaag aactcctggt agtcaaagtt aaagtgcggc
    15961 atttactgac gttataactt acccagttta gtgcgtaacc gtttaatggc ctggctgtgt
    16021 aactgactga cccgcgattc cccgacctcc agcaccgcgc caatctcttt gagattcagc
    16081 tcttcctggt aatagagggt taataccagt ttttcgcgct ccggcaacgt ttcgatggct
    16141 tccatcaccc gctggcgcag attactgtcc agcagttgtt gtagcgggtt ttctcgctga
    16201 tgatcatcag taaccagttc gatgctatcg ccgtgctctt cacgccactc atcgtaggag
    16261 aagagctggc tgttattggt gtcgagcagc atttgacgat aatcggcaat atcgatccct
    16321 aaacgttccg ctacctcagt ttccgtggcg ttgcggccaa gttcctgctc cagttgccct
    16381 attgcctgtg ccacttcacg cgcgttgcgt cgcacgctgc gcggcaccca gtcacggctg
    16441 cgaagttcat ccagcatagc gccacggata cgctgcactg cgtaagttgt aaatgccgtt
    16501 ccttgtgggg cgtcatagcg ttcgacagca ttaagtaacc caatgccgcc cgcctgtagc
    16561 agatcgtcaa gttccacgct cgcgggcagt cgaacctgca ggcgcaatgc ttcgtgacgc
    16621 accagcggga cataacgctg ccacagcgag tgtttatcca ttacaccttc agcggtatag
    16681 agtgaattca cgataaacag ccctgcgtta tatgagttat cggcatgatt atccgtttct
    16741 gcagggtttt taatcggacg attagtgggt gaaatgaggg gttatttggg ggttacgcaa
    16801 aaagccgaat gtaaaaaacc ccgtcggtgg cggggtttga gaggcattca gacgattaac
    16861 cctgcagcag agacagaacc tgctgcggta cctggttggc ttttgccagc acggagttac
    16921 cggcctgcag gataatctgc gctttcgaca tgttggacac ttcggtcgca tagtcggcgt
    16981 cctgaatacg ggactgcgct tcagacaggt tggtagtggt gttgttcagg ttggtgactg
    17041 cggaatccaa acggttttgc accgcaccca gggaggagcg gaatttgtcg atggagctga
    17101 tggcttcatc cagagctttc aggggatcgg ctgtcgtagc tgcttttgtt tcagcatctg
    17161 tcgtcaattt accatcagca tctttataaa ctgccttacc gctgccgtta gtcactgaac
    17221 catcatcctg taggtaaaaa tctacagtat cagatgtggt caacgcctcg ttgttatcaa
    17281 cataaaccga atcaacacct tttgttacag cgccagttgt gccgttgacg ctataacctg
    17341 cagtagtgaa atcaacattg tttcccgagg ttgatacagc tttatcgaaa gctgcctgat
    17401 caacttttgc acctgttact gaataagtaa tagtgtccgg cgtagtacta ttaccggtaa
    17461 aggagataga ggtaccgcca atgtcaatgg ttgtcgcttt ggtattattg gctgcaagtg
    17521 tggacagact agcctcagtc aaagaagcac cagagccgtt tttcgttaag cgcccagttg
    17581 tatcaatgta gagtttatct ccattgcttg ccgtaatttt accatcactg ctgatctgaa
    17641 catcctgtgc agaaccatcg atagtaatag tgcccttagc ggtatcacca actcccgggg
    17701 tcaaatattt ctgaacatcg gcagctgaag ccgttgtggt atcaaaagag tatgacttac
    17761 ttgcactgtc atacttgtaa ttcgttgcac tagccgccgt tccgaagccg ttattaatgg
    17821 ttgcctgaac agtatcacca tcactaactc cagccagcaa atcagacgct ttagcagcat
    17881 cgtaacccgc actcacagta tatttgttgc cgaccactgt tgcattagca gctaccaagt
    17941 cagctttaga tgcagcagta ttagccacag ccccgccacc attcacatta aacccattca
    18001 gccccagcgt atcagagtca attttcttca gatcaatagt gatagtctgg ccgtcattcg
    18061 caccaacctg aattttcatc gaaccgtctt ttgccagtac gttcacgccg ttgaactggg
    18121 tctgaccgga tacgcggtca atttcgtcga gacgggattt gatttcgtcc tgaatggagt
    18181 ccagatccga atcagagtta gtcccggtag aagcctgaac cgtcagctca cggatacgct
    18241 gtaagttgtt gttgatttcg gacagcgcgc cttcagtggt ctgtgcaaca gaaataccgt
    18301 cgttggcgtt acgtgcagcc tgagtcaggc ctttaatgtt agaagtaaaa cggttagcaa
    18361 tcgcctgacc cgcggcgtca tccttcgcgc tgttaatacg caagccagaa gacagacgct
    18421 cgatagaact cgacagcgca gactggttct tgttgatatt attttgagtg atcagcgaga
    18481 ggctgttggt attaatgact tgtgccatga ttcgttatcc tatattgcaa gtcgttgatt
    18541 acgttttggg tttccacccg tcggctcaat cgccgtcaac cctgttatcg tctgtcgtaa
    18601 aacaaccttt agaatttttt tcacaaacag ccattttttg ttagtcgacg aaatactctt
    18661 ttctctgccc cttattcccg ctattaaaaa aaacaattaa acgtaaactt tgcgcaattc
    18721 aggccgataa ccccggtatt cgttttacgt gtcgaaagat aaaaggaaat cgcatggcaa
    18781 gtatttcatc gctgggagtc gggtcaggtc tggatttaag ttcaatcctt gatagcctca
    18841 ccgccgcgca aaaagcgacg ctaaccccta tttcaaatca gcaatcgtcg tttaccgcta
    18901 aacttagcgc ctacggtacg ctgaaaagcg cgctgacgac tttccagacc gccaatactg
    18961 cattgtctaa agccgatctt ttttccgcca ccagcaccac cagcagcacc accgcgttca
    19021 gtgccaccac cgcgggtaac gccatcgccg ggaaatacac catcagcgtc acccatctgg
    19081 cgcaggcgca aaccctgacc acgcgcacca ccagagatga tacgaaaacg gcgatcgcca
    19141 ccagcgacag caaactcacc attcaacaag gcggcgacaa agatccgatt accattgata
    19201 tcagcgcggc taactcatcg ttaagcggga tccgtgatgc catcaacaac gcaaaagcgg
    19261 gcgtaagtgc gagcatcatt aacgtgggta acggtgaata tcgtctgtca gtcacatcaa
    19321 atgacaccgg ccttgataat gcgatgacac tctcggtcag cggtgatgat gcgctacaaa
    19381 gttttatggg ctatgacgcc agtgccagca gcaacggtat ggaggtctcg gtcgccgccc
    19441 agaatgcgca gctgacggtc aacaacgtcg ccatcgagaa cagcagcaac actatcagcg
    19501 acgcgctgga aaacatcacc ctgaacctga acgatgtcac cacgggcaac cagacgctaa
    19561 ccatcactca ggacacctcc aaagcgcaaa cggcgattaa agactgggtg aatgcctata
    19621 actcgctaat agataccttc agcagcctga ccaaatacac cgccgtagat gcgggagctg
    19681 atagccagag ttctagcaat ggcgcactgc tcggcgactc cacgctgcgg acgattcaga
    19741 cgcagttgaa gtcgatgctg agtaataccg tcagttcttc cagctataaa acgctggcgc
    19801 agattggtat cacgaccgat cccagcgatg gcaaactgga actggatgcc gacaaactca
    19861 ccgctgcact gaaaaaagat gccagcggcg taggtgcatt gattgttggc gatggtaaaa
    19921 aaaccggcat cacaaccacc atcggcagca acctgaccag ttggctttcg acaacgggca
    19981 ttattaaagc cgctaccgat ggcgttagta aaaccctgaa taagttaact aaagactaca
    20041 acgccgccag cgatcgcatt gatgcgcagg tcgcgcgcta caaagaacaa tttacccaac
    20101 tggacgtttt aatgacctcg ttaaacagca ccagcagcta cttaacgcag cagttcgaaa
    20161 acaacagtaa ttccaagtaa tcattattca tcgggagaca ggtcatgtac gcggcaaaag
    20221 gcacccaggc ctatgcgcaa attggcgtcg aaagcaccgt aatgagcgcc agccaacagc
    20281 agttggtcac catgctattt gatggagtgc tgagcgcact ggttagagcg cgactgttta
    20341 tgcaggacaa caatcagcaa ggcaaaggcg tctctttgtc aaaagcgatc aacatcattg
    20401 agaacggact gcgggtgagt cttgatgaag agagcaaaga cgaattaacc caaaacttaa
    20461 ttgctcttta tagctatatg gtcaggcgtt tgctgcaagc caatttacgc aacgatgtct
    20521 ccgcagtcga agaggtggaa gcattaatgc gtaatattgc cgatgcctgg aaagagtctt
    20581 tactctcccc ttcttcgatt caggacccag tctgatgaac aatgcaccgc atttgtattt
    20641 cgcctggcaa caactcgtcg aaaaaagcca gctcatgtta cgcctggcaa cggaagaaca
    20701 atgggatgaa ctcatcgcta gcgaaatggc gtatgtgaat gcggtgcagg agatcgcaca
    20761 cctgactgaa gaggttgccc cgtccactac gatgcaggag cagctccgcc cgatgctgca
    20821 cctgattctc gacaacgaaa gcaaggtaaa gcagttatta cagattcgga tggatgaact
    20881 ggcgaaactg gtcggtcagt catcggtgca aaaatcggtg ttaagtgcct atggcgatca
    20941 gggcggcttt gtgctggctc cgcaggataa cctcttttga atcttaatga gccgatggct
    21001 cgcgaataat ccgattacgg ctacgcttct aatattcccc ttgaatggag tcgaagaatg
    21061 cgtaatccca cgctgttaca atgttttcac tggtattacc cggaaggcgg taagctctgg
    21121 cctgaactgg ccgagcgcgc agacggtttt aatgatattg gtatcaatat ggtctggttg
    21181 ccgcccgcct ataaaggcgc atcgggcggc tattcggtcg gctacgactc ctatgattta
    21241 tttgatctag gtgagtttga tcagaaaggc agcatcccta ctaaatatgg tgataaagca
    21301 caactgctgg ccgccattga cgccctgaaa cgtaatgaca ttgcggtgct gctggacgtg
    21361 gtagtcaacc acaaaatggg cgcggatgaa aaagaagcta ttcgcgtgca gcgtgtaaat
    21421 gctgatgacc gtacgcaaat tgacgaagaa atcattgagt gtgaaggctg gacgcgttac
    21481 accttccccg ctcgtgccgg gcaatactcg cagtttatct gggatttcaa atgttttagc
    21541 ggtatcgacc atatcgaaca ccctgatgaa gatggcattt ttaaaattgt taacgactat
    21601 accggagaag gctggaacga tcaggttgat gatgaactcg gtaatttcga ttatctgatg
    21661 ggtgagaata tcgattttcg caatcatgcc gtgacggaag agattaaata ctgggcgcgc
    21721 tgggtgatgg aacaaacgca atgcgacggc tttcgtcttg atgcggtcaa acatattccg
    21781 gcatggtttt ataaagagtg gatcgaacac gtacaggaag ttgcgccaaa gccgctgttt
    21841 attgtggctg aatactggtc gcatgaagtc gataaactgc aaacgtatat tgatcaggtg
    21901 gaaggcaaaa ccatgctgtt tgatgcgccg ctgcagatga aattccatga agcatcgcgc
    21961 atgggtcgcg actacgacat gacgcagatc ttcactggga cgctggtgga agccgatcct
    22021 tttcacgccg taaccctcgt tgccaaccac gacacccaac cgttgcaagc cctcgaagca
    22081 ccggtcgaac cgtggtttaa accgctggcg tatgccttaa ttttgttgcg ggaaaatggc
    22141 gttccttcgg tgttctatcc ggacctctac ggtgcacatt acgaagatgt cggtggtgac
    22201 gggcaaacct atccgataga tatgccaata atcgaacagc ttgatgagtt aattctcgcc
    22261 cgtcagcgtt tcgcccacgg tgtacagaca ttatttttcg accatccgaa ctgcattgcc
    22321 tttagccgca gtggcaccga cgaatatccc ggctgcgtgg tggtcatgtc gaacggagat
    22381 gatggcgaaa aaaccattca tctgggagag aattacggca ataaaacctg gcgtgatttt
    22441 ctggggaacc ggcaagagag cgtagtgaca gacgaaaacg gcgaagcgac cttcttttgc
    22501 aacggcggca gcgtcagcgt gtgggttatc gaagaggtga tttaaatcca tccccggcgg
    22561 caagccgggg agatttcatt acggcagtgg cgtcggtaac ggcgctttat ccagcgcggc
    22621 ggcacactct gccgtcgggc gatccacacg ctccatcgtc ataccatcgt actcaatggt
    22681 gttaccgtca cgctcaacct cgtacagctc acgttttacg gtaacgttag tgagatcatc
    22741 agacatcaat gtcaacttac ccggcacggc aatcccccgc tgccactgac ggcaatccaa
    22801 ggtatcgccc tctttagtga caatcaggct gcctatcgct tccggactga ccagcgcacg
    22861 ttgcggccct ttggtctgcc agtagcccgc cagccagtcc ggcgcggggg ttttcacgac
    22921 attgttatag ttttcgactt cggcacagcc cgccagcaac atgagcgcgc ctgcaattgc
    22981 gagttttttc atcattcgtc ctgcatgaga agaaaagagg attgtggcat taaagctctg
    23041 atgtcgccag cctttccagg ttcagagaga aatttgatct aactattatt tttgtgaatt
    23101 attaccccga aaataaattt gcatggtgta tgattcgcgg ctcttgtttc aaaccttctg
    23161 agttcagagg ctacattcat gtcatggcag caattcaaac acgcctggtt gattaaattc
    23221 tgggcaccca tccctgcggt catcgcggcg ggtattctct ccacttacta ttttggcatt
    23281 actggcacct tttgggctgt cacgggtgaa tttacccgtt ggggcggtca gctcctgcag
    23341 ctgtttggcg tccatgctga agagtggggt tattttaaaa ttatccatct ggaaggatcg
    23401 ccattaaccc gcatcgacgg catgatgatc ctcggtatgt ttggcggctg ctttgctgct
    23461 gcgctgtggg ccaacaatgt caaactacgc atgccacgca gccgtatccg cattatgcag
    23521 gccatcattg gcggcattat cgccggtttt ggcgcgcgtc tggcaatggg ctgtaacctg
    23581 gcggcgttct ttaccggcat tcctcagttc tcgctgcatg cctggttctt tgccatcgcc
    23641 actgccattg gttcatggtt tggcgcgcgc tttaccctgc tgcccatctt ccgtattccc
    23701 gtgaaaatgc agaaagtttc tgccgcctca ccgctgacgc aaaaaccgga tcaggcgcgg
    23761 cgtcgttttc gtctcgggat gctggtcttt ttcggcatgc tgggctgggc gctgctcacg
    23821 gcgatgaacc aaccaaaact ggggctggca atgctgtttg gcgtcggctt tggtttactg
    23881 attgaacgtg cgcaaatctg ctttacttcg gcgttccgcg atatgtggat caccggacgt
    23941 acccatatgg cgaaagcaat cattatcggt atggcggtga gtgccatcgg gatcttcagt
    24001 tacgtacagt taggcgttga acctaaaatc atgtgggcgg gaccaaatgc ggtaattggt
    24061 ggtttacttt ttggttttgg catcgtgctg gcaggcggct gcgaaaccgg ctggatgtac
    24121 cgcgcggtag aaggccaggt gcactactgg tgggtcggtc tgggcaacgt gatcggctca
    24181 acgattctgg cgtattactg ggatgatttc gctccggcgc tggccaccga ctgggacaaa
    24241 atcaacctgc tgaaaacttt tggtccgatg ggaggcctgc tggtgacata tttgctgctg
    24301 tttgctgcgc tgatgttgat tatcggctgg gaaaaacgct tcttccgccg tgcggcaccg
    24361 cagactgcta aggagatcgc atgaaaaata tcgttcctga ttaccgcctt gatatggtgg
    24421 gtgaaccctg cccttatccg gcagtcgcaa cccttgaggc gatgccgcaa ttaaaaaaag
    24481 gtgagatcct ggaagtggtg agcgactgtc cgcagtcgat caataatatt ccactggatg
    24541 cgcgtaatca cggctacact gtgctggata ttcagcaaga cgggccgacc attcgttatt
    24601 taattcagaa gtaatccctc attcccgctg tggcccacag cgggaacatc gttacctctg
    24661 acacatagat tttacccgcc ccctctccta ttcttttcag tatgatctca ggaggttgct
    24721 atgtgtggac gctgtgccca atcccaaacg cgtgaggatt accttgcgct tctcgcggaa
    24781 gatattgaac gcgatattcc ctacgatccc gaacccattg gcagatacaa tgtcgcgccg
    24841 ggaaccaaag ttcttctgct cagtgaacgt gatgaacacc ttcatctgga tccggttttc
    24901 tggggatatg ctcccggatg gtgggataaa ccgccgctga ttaacgcccg cgtagaaact
    24961 gcctcaacca gtcgcatgtt taaaccgctc tggcaacatg gtcgggcaat ctgttttgcc
    25021 gatggctggt ttgagtggaa aaaagaaggc gacaaaaaaa gccttatttt atctatcgcg
    25081 ctgacggaca acctgttttt atggccgcga taggtagcat accgtttgag cgtggtgacg
    25141 aagccgaagg atttttgatc gtcactgcgg cggcagatca aggtctggta gatattcatg
    25201 accgccgccc gctggttctg tcgccagaag ctgcacggga atggatgcgg caagacattg
    25261 gtggtaaaga agcctcagaa atcgctacca gaagctgtgt gccagcaaac cagttcacct
    25321 ggcaccctgt gtcgcgcacg gtgggtaatg tcaaaaacca gggcgcggag ttaattcaac
    25381 ctgtttgttg acatcctcca cgccctgaat gacgaggccc cctgctacgt tcaggctgtc
    25441 gcctgaatca tttcggtggg ttcctgcttc aacgagcggc ctgactgcac catctctcca
    25501 caggcaagaa cggcgtgccc cgccgctaaa atgttacgag cgccatttac atcggcgttc
    25561 gctgtatatc cacatacctg gcatctgaat ttactttgtg acaggcgatt ttctttcgct
    25621 gtatgaccac agcacgcgca acgctggctt gtgtacgctg gcggaacagc aagcacctga
    25681 ccgccacgcc agagctgctt gtactcaagc tggcggcgca tttcatacca gccctgatcc
    25741 agtatcgaac ggtttaaacc tgattttgcc cggacattgc gccccggctg gctgaccgta
    25801 cccgctgctg actttgacat gttgctgacc ttcaaatcct caatgactat cattacgtgg
    25861 tttttgctga cggtcgttgt gactttgtga aggtagtccc tgcggatatt tgcgatacag
    25921 gaatgcagtc gctgtatttt gcgtttctgc ttctgccagt tgttgctgaa tttgaccttg
    25981 cggcttaact gtcgctgaag tctcgccagc ttcttctggt ttttctgaaa actgtttaca
    26041 ggctcaaaga ctgtgccatc tgacagcgag gcaagtttag ccacgccagc atccagcccg
    26101 accattgatg ctgaagggtg aaccggagtt gatacttcac tttctgcctg aatactgatg
    26161 taccacttac cgcaggactg gctgacagtg acatttttca caacacccgt gacctgacgg
    26221 ctgttccggt agcgcatcca gccaagtttt ggcagaaaaa tacggctgtt ttcctgatcg
    26281 agcttaacgc cctgcggata gcggaatgca tcattctgtc accgcttttt gaatcagggg
    26341 aacgccgacc gcttctggaa aaagtttttg caggcctgct cagggtcttt cagtgactgt
    26401 tgcaatggct gtgacggagc atctttaatc cattgcgttt cagtggcatt tttccactca
    26461 accaatcagg aagccatttt cgcgtaaggg atgtctttgt taccggcctt atgattctca
    26521 ttctgacgtg ccagagtacg actgaaaacg aaacgacatg cgcctgcgaa gcgcctcatt
    26581 tcacgcgcct gttgaccacc gggtcttaac cggaatttga atacctgtag tcgtttcaca
    26641 gggcaaatta tactttggtc tgtgaaaaaa actgatatcc gacgcggaag gcactgtgtt
    26701 ttcctgatgc atgctcaccg ggtctttgtt accaagtacc gacgccagat ttttgaccat
    26761 gacgcgacag aaaaactacg tacttacttt tcaaatgtat gtgctgattt tgaagctgaa
    26821 ctggttgaaa tggatggcga accagagcac gtccatctgt taattaacta tccccccaaa
    26881 ctggcgatat ccagtctggt aaacaaacag cctcaaaggc gtatcgggta ggttactgcg
    26941 acgggatcgg ccagatattg ctgccaggta ttaccgcaaa gatgttttgt ggcgtcccgg
    27001 ctattttgcc agtagctgcg gaggtgcgcc aatatccgtc atccgccaat acattgaaca
    27061 acagcaaaca cctggtcagg tgaaaaaacg agccttatat ccccgccctg aaggacgggc
    27121 ttttacggtg cactggataa gcctggagat tatctacacc tgcatggaca tcattgtact
    27181 atgccccgta catgccccat cacatcctca acatcaaagt attagagcct tgcaaaccat
    27241 tatgcgtctc agtttgtctc atctatctta cgcaaaaaaa atagaactaa tatcaagcgg
    27301 cgctatattg cgtattaaat aaaaccacct caattacatt aataaatcta aataaatatc
    27361 atgcaaataa tttccatggt tatttttagg ctatttaaat taaaaaaata aattatgagt
    27421 caatcataaa taacaacctt taatataatt tgactaccaa atttatattc gttcacaaaa
    27481 tagtcatgca ttgtgtgcaa tagaaacagt tcagataaaa atagggatct acttcacaat
    27541 ttaaatgaga aactaaaact tacatcttga aataatctca ttgattggat gaatatttgt
    27601 agtgcagtga catcattttt aaataatagt tcaaataaaa gggctcacga tgaaaaaatt
    27661 aacagtggca atttctgctg tagctgcatc agtactgatg gcgatgtctg ctcaggcagc
    27721 agagatttac aataaagaca gtaacaagct ggatctgtac ggaaaagtta atgctaagca
    27781 ctacttctct tctaacgatg cagatgatgg cgacacaact tatgttcgtc tgggcttcaa
    27841 aggcgaaact caaatcaacg atcaactgac tggtttcggt cagtgggaat atgaattcaa
    27901 aggcaaccgt gctgaatctc aaggttcttc caaagataaa acccgtcttg ccttcgctgg
    27961 cctgaaattc ggtgactacg gctccatcga ttatggccgt aactacggtg tagcatacga
    28021 tatcggtgca tggaccgacg ttctgccaga attcggtggc gatacctgga cccaaacaga
    28081 tgtgttcatg actggtcgca ccacaggtgt tgcaacctat cgtaacaacg acttctttgg
    28141 tctggttgat ggtctgaact ttgctgctca gtatcagggt aaaaatgacc gcactgacgt
    28201 aactgaagct aacggtgatg gtttcggttt ctccactact tatgagtatg aaggattcgg
    28261 tgtaggtgca acctatgcta aatctgaccg cactaatgat caggttatct acggtaacaa
    28321 cagcctgaat gcatctggtc aaaatgctga agtatgggca gctggtctga aatatgatgc
    28381 gaacaacatc tatctggcta ccacctattc tgaaacccag aacatgactg tttttggtaa
    28441 taaccatatt gccaacaaag cacaaaactt cgaagtagtt gcacaatatc agttcgactt
    28501 cggtctgcgt ccgtccgttg cttacctgca atctaaagga aaagacttgg gtgcgtgggg
    28561 tgatcaggac ctgattgaat atattgatgt aggtgcaacc tattacttca acaaaaatat
    28621 gtccactttt gttgattaca aaatcaacct gattgataag agcgatttca cgaaagcatc
    28681 tggcgttgct accgatgata tcgttgctgt aggattggtt taccagttct aatctgatta
    28741 ctcaaagata tgttgtggga ggctttgcct ccccaacata taagtggcgc cctcaagcca
    28801 ctttccttag aagcactatc ttgcttctga ctatataaat taccctttat tttctgggac
    28861 gtttcaacgc cccattttta ataatactca gtgaacaatt aacgtatcaa ttagatttaa
    28921 taacaacgat atccgtacct aacctgatat ctaatgtcat aagcatccct ttaattatcc
    28981 cctcaacctc ttgtaaattt cccactcttg agctccacat agtccaaacg ccggatgcaa
    29041 aaaaaatcac acgtgcatct ctgaaattgt gatggaggaa gtgagtatca attatatgtg
    29101 cttataaacg ttcccgattg gcacagaagt caccggattg ttcaggctcc aataggttga
    29161 gtatgtcgta tttattttcc aaaataaaaa tcgatgctgt tgctccttaa taaaatactt
    29221 ttactctctg gacgcttttc ttagctctct tggtgttatc ttaaaacgat tatggaacct
    29281 ttcagtaaaa cgagaaggac acttgtaacc attttccctg gcaatctcgc tgatgggttt
    29341 tactgtcgtt tgtatagcag acaacgcatt atttaacctc acgtcgtcca atatactttg
    29401 aaaacttatt ccctcgct
//
LOCUS       DAETPZ010000045        29088 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.045, whole genome shotgun sequence.
ACCESSION   DAETPZ010000045 DAETPZ010000000
VERSION     DAETPZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.045"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            76..1353
                     /gene="waaA"
                     /locus_tag="K0W59_002274"
     CDS             76..1353
                     /gene="waaA"
                     /locus_tag="K0W59_002274"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HBI3023287.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1361..1840
                     /gene="coaD"
                     /locus_tag="K0W59_002275"
     CDS             1361..1840
                     /gene="coaD"
                     /locus_tag="K0W59_002275"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBI3023288.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1879..2688)
                     /gene="mutM"
                     /locus_tag="K0W59_002276"
     CDS             complement(1879..2688)
                     /gene="mutM"
                     /locus_tag="K0W59_002276"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HBI3023289.1"
                     /translation="MPELPEVETSRRGIEPNLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2786..2953)
                     /gene="rpmG"
                     /locus_tag="K0W59_002277"
     CDS             complement(2786..2953)
                     /gene="rpmG"
                     /locus_tag="K0W59_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBI3023290.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2974..3210)
                     /gene="rpmB"
                     /locus_tag="K0W59_002278"
     CDS             complement(2974..3210)
                     /gene="rpmB"
                     /locus_tag="K0W59_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBI3023291.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3427..4095)
                     /gene="radC"
                     /locus_tag="K0W59_002279"
                     /pseudo
     CDS             complement(3427..4095)
                     /gene="radC"
                     /locus_tag="K0W59_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            4267..5487
                     /gene="coaBC"
                     /locus_tag="K0W59_002280"
     CDS             4267..5487
                     /gene="coaBC"
                     /locus_tag="K0W59_002280"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBI3023292.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5465..5923
                     /gene="dut"
                     /locus_tag="K0W59_002281"
     CDS             5465..5923
                     /gene="dut"
                     /locus_tag="K0W59_002281"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBI3023293.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6030..6626
                     /gene="slmA"
                     /locus_tag="K0W59_002282"
     CDS             6030..6626
                     /gene="slmA"
                     /locus_tag="K0W59_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HBI3023294.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6663..7304)
                     /gene="pyrE"
                     /locus_tag="K0W59_002283"
     CDS             complement(6663..7304)
                     /gene="pyrE"
                     /locus_tag="K0W59_002283"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBI3023295.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7370..8086)
                     /gene="rph"
                     /locus_tag="K0W59_002284"
     CDS             complement(7370..8086)
                     /gene="rph"
                     /locus_tag="K0W59_002284"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBI3023296.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8213..9076
                     /locus_tag="K0W59_002285"
     CDS             8213..9076
                     /locus_tag="K0W59_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBI3023297.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9297..10121
                     /gene="dinD"
                     /locus_tag="K0W59_002286"
     CDS             9297..10121
                     /gene="dinD"
                     /locus_tag="K0W59_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HBI3023298.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            10412..11029
                     /locus_tag="K0W59_002287"
     CDS             10412..11029
                     /locus_tag="K0W59_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HBI3023299.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11026..12708)
                     /gene="ligB"
                     /locus_tag="K0W59_002288"
     CDS             complement(11026..12708)
                     /gene="ligB"
                     /locus_tag="K0W59_002288"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HBI3023300.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12966..13589
                     /gene="gmk"
                     /locus_tag="K0W59_002289"
     CDS             12966..13589
                     /gene="gmk"
                     /locus_tag="K0W59_002289"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBI3023301.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13644..13919
                     /gene="rpoZ"
                     /locus_tag="K0W59_002290"
     CDS             13644..13919
                     /gene="rpoZ"
                     /locus_tag="K0W59_002290"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBI3023302.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            13938..16046
                     /gene="spoT"
                     /locus_tag="K0W59_002291"
     CDS             13938..16046
                     /gene="spoT"
                     /locus_tag="K0W59_002291"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HBI3023303.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16053..16742
                     /gene="trmH"
                     /locus_tag="K0W59_002292"
     CDS             16053..16742
                     /gene="trmH"
                     /locus_tag="K0W59_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HBI3023304.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16748..18829
                     /gene="recG"
                     /locus_tag="K0W59_002293"
     CDS             16748..18829
                     /gene="recG"
                     /locus_tag="K0W59_002293"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBI3023305.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18863..20068)
                     /gene="gltS"
                     /locus_tag="K0W59_002294"
     CDS             complement(18863..20068)
                     /gene="gltS"
                     /locus_tag="K0W59_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HBI3023306.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLRLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20348..21739
                     /gene="xanP"
                     /locus_tag="K0W59_002295"
     CDS             20348..21739
                     /gene="xanP"
                     /locus_tag="K0W59_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HBI3023307.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSDFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21860..23569
                     /locus_tag="K0W59_002296"
     CDS             21860..23569
                     /locus_tag="K0W59_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBI3023308.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNREWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     QLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23622..25940)
                     /gene="yicI"
                     /locus_tag="K0W59_002297"
     CDS             complement(23622..25940)
                     /gene="yicI"
                     /locus_tag="K0W59_002297"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HBI3023309.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEYFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25950..27333)
                     /locus_tag="K0W59_002298"
                     /pseudo
     CDS             complement(25950..27333)
                     /locus_tag="K0W59_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27626..27720
                     /locus_tag="K0W59_002299"
     tRNA            27626..27720
                     /locus_tag="K0W59_002299"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27660..27662,aa:Sec,seq:tca)
     gene            28020..>29088
                     /locus_tag="K0W59_002300"
     CDS             28020..>29088
                     /locus_tag="K0W59_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBI3023310.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAH"
BASE COUNT         7185 a         7795 c         7312 g         6796 t
ORIGIN      
        1 aaacgggaaa agtaatggta aagccacagc taaatacata gaatccccag cacatccata
       61 agtcagctat ttactatgct cgaattgctt tacaccgccc ttctctacct tattcagccg
      121 ctgatctgga tacggctctg ggtgcgcgga cgtaaggctc cggcctatcg aaaacgctgg
      181 ggtgaacgtt acggttttta ccgccatccg ctaaaaccag gcggcattat gctgcactcc
      241 gtctccgtcg gtgaaactct ggcggcgatc ccgttggtgc gcgcgctgcg tcatcgttat
      301 cctgatttac cgattaccgt tacaaccatg acgccaaccg gttcggagcg cgtacaatcg
      361 gctttcggga aggatgttca gcacgtttat ctgccgtatg acctgcccga tgcgctcaac
      421 cgtttcctga ataaagtcga ccccaaactg gtgttgatta tggaaaccga actatggcct
      481 aacctgattg cggcgctaca taaacgtaaa attccgctgg tgatcgctaa cgcgcgactc
      541 tctgcccgct cggccgcagg ttatgccaaa ctgggtaaat tcgtccgccg cttgctgcgt
      601 cgtattacgc tgattgctgc gcaaaatgaa gaagatggtg cacgttttgt ggcgctgggc
      661 gcaaaaaata accaggtaac cgttaccggt agcctgaaat tcgatatttc tgtaactccg
      721 cagttggctg ctaaagccgt gacgctacgc cgccaatggg caccacaccg cccggtatgg
      781 attgccacca gcactcacga aggtgaagag agcgtggtca ttgccgcaca tcaggcattg
      841 ttacagcaat tcccgaattt attgctcatc ctggtacccc gtcatccaga acgcttcccg
      901 gatgcgatta accttgtccg ccaggctgga ctaagctata tcacacgctc ttcaggggaa
      961 gtcccctcca ccagcacgca ggttgtggtt ggcgatacga tgggcgagtt gatgttactg
     1021 tacggcattg ccgatctcgc ctttgttggc ggttcactgg ttgaacgtgg tgggcataat
     1081 ccgctggaag ctgccgcaca cgctattccg gtattgatgg ggccgcatac ttttaacttt
     1141 aaagacattt gcgcgcggct ggagcaggca agcgggctta ttaccgttac cgatgccact
     1201 acgcttgcaa aagaggtttc ctctttactc accgacgccg tttaccgtag tttctatggc
     1261 cgtcatgccg ttgaagtact gtatcaaaac cagggcgcgc tacagcgtct gcttcaactg
     1321 ctggaacctt acctgccacc gaaaacgcat tgaggttgtt atgcaaaaac gggcgattta
     1381 tccgggtact ttcgatccca ttaccaatgg tcatatcgat atcgtgacgc gcgccacgca
     1441 gatgttcgat cacgttattc tggcgattgc cgccagcccc agtaaaaaac cgatgtttac
     1501 cctggaagag cgtgtggcac tggcacagca ggcaaccgcg catctgggga acgtggaagt
     1561 ggtcgggttt agtgatttga tggcgaactt cgcccgtaat caacacgcta cggtgctgat
     1621 tcgtggcctg cgtgcggtgg cggattttga atatgaaatg cagctggcgc atatgaatcg
     1681 ccacttaatg ccggaactgg aaagtgtgtt tctgatgccg tcgaaagagt ggtcgtttat
     1741 ctcttcatcg ttggtgaaag aggtggcgcg ccatcagggc gatgtcaccc atttcctgcc
     1801 ggagaatgtc catcaggcgc tgatggcaaa attagcgtag cgtttatgcc ggatggtatg
     1861 ccatccggct cgcatgaatt acttctggca ctgccgacaa taaaacgttg cccgctgcgc
     1921 atgtttagtc gccacaatcg gcgtaccgca cacccgacac ggctcacctt ttcgcccgta
     1981 aacctgcaat tcctgcgcga aatagcccgg tttaccatca ctttgcagaa aatctttcag
     2041 cgttgtacca ccctgctcaa tcgaacgcag caacaccgct ttaatcaccc gagctaacaa
     2101 ttcacactct gccagcgaca gtgatgacgc cagccgatcc ggatggatcc ccgccgcaaa
     2161 cagtgattcg ctggcataga tattccctac ccctaccacc agcttgttat ccatcagcca
     2221 cggtttaatc gccgtttttt tcttcgcgca cttctgatgc agatactcac cattgaaatc
     2281 gtcgctaagc ggctccggtc caagatgggc cagcacatta tgcccttcca gctctttggt
     2341 ccacagccag gcaccaaagc ggcgcggatc ggtgtagcgc agcactttac cgttgctcat
     2401 caccaaatcc acatggtcat gcttttcagg gggaagttct tctggaagga tgcgcaggct
     2461 gccagacatc cccaaatgaa tgataatcca gccctcaggc agctccagca gcagatattt
     2521 agcccgccgc tgcacgctaa gcactggttg atcgcttaat cggtagatct cttctgaaac
     2581 cggccagcgc aagcgtccgt tgcgcaccac cgcatgaaga atggttgcac caacgagatt
     2641 cggttctatg ccgcgacggc tggtttcaac ttcgggtaat tcaggcatag catctccagg
     2701 aatgaacaga tgcaatcaat atgggggcaa gcagataaca aaaaaccccg ccggagcgag
     2761 gtttttcgtt acatcaaagc gagaattatt tgattttcgc ttctttgtag atcacgtgct
     2821 gacgaacaac tggatcgaat tttttcagtt ccagtttttc cggcttagta cgtttgttct
     2881 tcgtagtggt atagaagtga ccagtaccag cagaagaaac cagcttgatt ttctcacgaa
     2941 tacctttagc catgatttat ttcctctaag tacttagtac ttttcgccac gggcacgcag
     3001 ttcagccaga actgtatcga tgcctttttt atcgattaca cgcatacctt tagcagatac
     3061 gcgcagggtg acaaaacgct tctcgctctc aacccagaaa cggtgagagt gcaggttcgg
     3121 caggaaacgg cgtttagtcg cgttcagtgc gtgggaacgg ttgttaccgg tcaccggacg
     3181 cttgccagta acttggcaga ctcgggacat gtctattctc caaaaatcaa attagctcga
     3241 gcttcgtatg gggtattggc gcctcgtcag gctttacagc ccggtcatcg cagttctatg
     3301 tgaactctcg attgccaggc ccaaatgcca aacccgagat tctcaaaggt ggcgtagtat
     3361 acgctgactc agcgatgtgc tcaagtcccg aacagacaaa gatcccgaag gatcgcgcat
     3421 agcgggttaa atccagccgc gttcggcaaa agaaacatac tctccacgcc caatcacgat
     3481 atggtcgagc acgcgtaaat ccatgaactg acaactcttt attatccgtt cagtaatgag
     3541 tttatccgct ttactgggtt cagcacaacc cgaagggtga ttatgtgcaa ggatcagcgc
     3601 cgaggcgttt atttttatcg cttagcggat aatttcccga ggatggactt caacatggtt
     3661 tagcgtgccg gaaaaaagac ggctatgcgt tataacccgg tgttgtgagt cgagaaagat
     3721 caccataaag atctcccgct cctcacccgt gagctggctt tgtaaaaatt cccgcgtcat
     3781 ctccgggctg agtaaagggc tttcttcacg catccgcacg ttgtagtaac gtcgcgccag
     3841 ttcagcaatc ccctttaact gggcaaattt cgctacgcca attccatgaa caccactaaa
     3901 ttgttcatat tcagaggtta acaagccata aagagagccg aaattctcca gcatctcttt
     3961 tgccagggtt aatacatctt taccgcgcgt tccggtacgc agaaatagcg ccagcagctc
     4021 gacatccgtt aaggcgctaa taccaaactt cagcattttt tcgcgcggca tcaacagctg
     4081 tgcattgttt ttcaccttca cctcctttgt ggtgctcgca tcctgtcata gccgttccgt
     4141 taaatcgacg gccagttttc aatcctggaa agagcctcgc aaagtgaatc tcaggtgaag
     4201 gcacgacaac aaaagtattg tgataaaatc gccaacttct ggtgtcaccc aacaggaaaa
     4261 atcatcatga gcctggccgg taaaaaaatc gttctcggcg ttagcggcgg tattgctgcc
     4321 tataaaaccc ctgaactggt gcgtcgtttg cgcgatcgcg gggccgacgt ccgcgtagcc
     4381 atgaccgaag cggcaaaagc ctttatcacc ccacttagct tgcaggcggt ttctggttat
     4441 cccgtttccg acagtctgct tgacccggca gccgaagccg ctatgggcca tattgagctg
     4501 ggtaaatggg ctgatttagt gattctcgcc cctgccacgg cagatttgat tgcccgtgtt
     4561 gctgccggaa tggcgaatga cctggtatcg acgatttgtc tggctacacc tgcgcctgta
     4621 gccgtgctcc ccgccatgaa ccagcagatg taccgtgccg ctgccacgca gcataattta
     4681 gaggtgcttg cttcccgtgg tttgctcatc tgggggccag acagtggcag tcaggcttgt
     4741 ggtgatatcg gtcctggacg aatgctcgat ccgttaacca ttgtggatat ggcggtggcg
     4801 catttttcgc ccgtcaacga cctgaaacat ctgaacatta tgattaccgc cggtccgacg
     4861 cgtgaaccgc tcgatccggt gcgttatatc tctaatcaca gctccggcaa gatgggtttt
     4921 gctatcgccg ccgctgctgc ccgtcgtggc gcgaacgtca cgctggtatc aggtccagtt
     4981 tcactaccga cgccaccgtt tgttaaacgt gttgatgtga tgaccgcgct ggaaatggaa
     5041 gccgccgtga atgcttctgt acagcagcaa aatattttta tcggttgcgc cgccgtggcg
     5101 gattatcgcg cagctaccgt ggccccagaa aaaatcaaaa agcaggccac gcagggtgat
     5161 gaattaacaa taaaaatggt taaaaacccc gatatcgtcg caggcgttgc cgcactaaaa
     5221 gaccatcgac cctacgtcgt tggatttgcc gccgaaacaa ataatgtgga agaatacgcc
     5281 cggcaaaaac gtatccgtaa aaaccttgat ctgatctgcg cgaacgatgt ttcccagcca
     5341 actcaaggat ttaacagcga caacaacgca ttacaccttt tctggcagga cggagataaa
     5401 gtcttaccgc ttgagcgcaa agagctcctt ggccaattat tactcgacga gatcgtgacc
     5461 cgttatgatg aaaaaaatcg acgttaagat tctggacccg cgcgttggga aggaatttcc
     5521 gctcccgact tatgccacct ctggctctgc cggacttgac ctgcgtgcct gtctcgacga
     5581 cgccgtagaa ctggctccgg gtgacactac gctggttccg accgggctgg cgattcatat
     5641 tgccgatcct tcactggcgg caatgatgct gccgcgctcc ggattgggac ataagcacgg
     5701 tatcgtgctt ggtaacctgg taggattgat cgattccgac tatcagggcc agttgatgat
     5761 ttccgtgtgg aaccgtggtc aggacagctt caccattcaa cctggcgaac gcatcgccca
     5821 gatgattttt gttccggtag tacaggctga atttaatctg gtggaagatt tcgacgccac
     5881 cgaccgcggt gaaggcggct ttggtcactc tggtcgtcag taacacatac gcatccgaat
     5941 aacgtcataa catagccgca aacatttcgt ttgcggtcat agcgtgggtg ccgcctggca
     6001 agtgcttatt ttcaggggta ttttgtaaca tggcagaaaa acaaactgcg aaaaggaacc
     6061 gtcgcgagga aatacttcag tctctggcgc tgatgctgga atccagcgat ggaagccaac
     6121 gtatcacgac ggcaaaactg gccgcctctg tcggcgtttc cgaagcggca ctgtatcgcc
     6181 acttccccag taagacccgc atgttcgata gcctgattga gtttatcgaa gatagcctga
     6241 ttactcgcat caacctgatt ctgaaagatg agaaagacac cacagcgcgc ctgcgtctga
     6301 ttgtgttgct gcttctcggt tttggtgagc gtaatcctgg cctgacccgc atcctcactg
     6361 gtcatgcgct aatgtttgaa caggatcgcc tgcaagggcg catcaaccag ctgttcgagc
     6421 gtattgaagc gcagctgcgc caggtattgc gtgaaaagag aatgcgtgag ggtgaaggtt
     6481 acaccaccga tgaaaccctg ctggcaagcc agatcctggc cttctgtgaa ggtatgctgt
     6541 cacgttttgt ccgcagcgaa tttaaatacc gcccgacgga tgattttgac gcccgctggc
     6601 cgctaattgc cgcgcagttg cagtaatatg acgccggatg acttttcatc cggcgagttt
     6661 ctttaaacgc caaactcttc gcgataggcc ttaaccgccg ccagatgttc cgccatttcc
     6721 ggcttctctt ccagataggc aatcagatct ttcagggtga tgatagagat cactttgcag
     6781 ttgtaatcac gctcaacttc ctgaatcgcc gaaatctcgc cgcgcccgcg ttcctgacga
     6841 tcgagcgaaa tcaacacgcc agcaagcgtc gcgccattgg cctgaataat ctccatcgac
     6901 tcgcgaatcg ccgttccggc ggtgatcaca tcatctacca gcattacgcg tccttgtaac
     6961 gcgctaccaa ccagattgcc gccttcaccg tggtcttttg cttctttgcg gttaaagcag
     7021 tacggcaggt ccaggtcgtg atgctccgcc agtgccacag cggttgtggt ggcaatcggg
     7081 atccctttgt aagcagggcc aaacagcaga tcgaactcaa tgccggaatc caccaacgct
     7141 tcagcgtaaa aacggcctaa cagtgccaga tcgcgcccgg tattaaacag cccggcgttg
     7201 aagaaatagg ggcttttgcg cccggatttc agcgtaaact cgccaaactt taacacctgc
     7261 ttgctaagcg caaattcaat aaactggcgc tgatatggtt tcatgccttc gctcctcatc
     7321 ttacttttct acagacaaaa aaaaggcgac tcatcagtcg ccttaaaaat cagtttgcca
     7381 gcgccgcctt ctgcgtcgct acaatggatt cgattccccc tcgggccagc gccaacaagg
     7441 tgagtagctc ttcatgggtg aacggctcgc cttctgccgt cccctgcact tcaatgatgc
     7501 gcccgtcttc ggtcatcact acgttcatgt cggtctctgc ggctgagtct tcaacgtatt
     7561 ccagatcgca aattgcttca ccgtttacaa taccgacaga aactgcggct accatccctt
     7621 tcatcgggtt ggttttcagc ttgccgtttt ccaccagctt ctgtagcgca tctgccagcg
     7681 ccacgcaggc acccgtaatc gacgcggtac gagtgccacc atcagcctga agcacgtcgc
     7741 agtccagcgt aatggtgaac tcacccagcg ctttcaaatc tactgccgcg cgaagagcac
     7801 gggcgatcag acgctggatt tccattgtgc gtccaccctg cttacctttc gccgcttcac
     7861 gagcgttacg ggtgtgggta gaacgtggca gcatgccgta ctctgcggtg atccagccct
     7921 ggccctgacc tttcaggaag cgcggcacgc cttcttcaat agaggcggta cacaatactt
     7981 tggtatcgcc aaattcgacc agcaccgagc cttctgcatg ttttgtatag ttacgagtca
     8041 gggtaacggg acgcacctga ttattgctac ggcctgctgg acgcatattg aaatctccgg
     8101 cttgaaacaa atgtggctgc gcattatacg gacttccggc ggttattcct atcctgacaa
     8161 ggcatcgatg gctataatcc ttccacctct ccttttataa acaggaacgt ctatgatccg
     8221 cagtatgacc gcctacgccc ggcgtgaaat caagggtgaa tgggggagcg caacttggga
     8281 aatgcgctcg gtaaaccagc gttatctgga aacttacttt cgtctgccgg agcagttccg
     8341 tagccttgaa cctgtcgttc gcgagcgtat tcgttctcgc ctgacgcgcg gtaaagtgga
     8401 atgtaccctg cgctatgagc cagatgttag cgcacaaggt gagctgatcc tcaatgaaaa
     8461 actggctaaa cagctggtaa ctgccgcgaa ctgggtaaaa atgcagagtg acgaagggga
     8521 aatcaacccg gttgatattc tacgctggcc gggcgtgatg gcagcccagg agcaggatct
     8581 cgacgcgatt gccgctgaaa ttctcgcggc gttggatggt acgctggacg actttatcgt
     8641 cgcgcgcgaa accgaaggtc aggcactgaa agcattgatc gagcagcgtc tggaaggcgt
     8701 caccgccgaa gtggtcaaag tccgcgccca tatgccggaa attctgcaat ggcagcgtga
     8761 gcgtctggtc gcgaagctgg aagatgctca ggttcagttg gaaaacaacc gtctggagca
     8821 ggagttggtt ctgctggcac aacgaattga cgttgccgaa gagctggacc gcctcgaagc
     8881 gcatgttaaa gagacgtaca acattctgaa gaaaaaagaa gcggttggtc gtcgtctgga
     8941 ctttatgatg caggagttca accgtgagtc gaacactctt gcgtcgaaat ctatcaatgc
     9001 cgaagtgaca aactccgcca tcgagctgaa agtgttgatt gagcagatgc gcgagcagat
     9061 tcagaatatt gagtaactcg taacaccaat tctgactttt cagccttcac aaatgccgcc
     9121 cactcaaaca gagcggcatt tttcttcccc gcaacttcaa ttctgttttg cgtgcctgct
     9181 ccagattttg cgatgttttt ttgcccagca cactgagaac gtgagatact cacaactgta
     9241 tataaataca gttacagatt tactttcttt gcaatcgata tcacatggag tgggcaatga
     9301 acgaacatca tcaacctttt gaagagataa aactgattaa tgcaaacgga gcagaacaat
     9361 ggtcagcaag acaacttggg aaactactgg gttattcaga gtatcgtcac tttatacctg
     9421 tattaacgcg cgccaaagag gcctgtgaga acagtggtca cacaattgat gaccatttcg
     9481 aggagatcct cgatatggtc aaaattggct caaatgccaa aagagcatta aaagacatcg
     9541 tactctcccg ctatgcctgt tacctggtag tacaaaacgg cgaccccgcg aaaccggtca
     9601 ttgcggcagg gcagacttat tttactatcc agacccgacg gcaggagctt gctgacgatg
     9661 aagcatttaa gcaacttcgt gaagacgaaa agcgtctgtt tctgagaaat gagttgaaag
     9721 aacataacaa acaattggtt gaggcagcac agcaagctgg ggtagcgaca gctactgatt
     9781 tcgccatatt tcagaatcat ggttaccagg ggctgtatgg tggattagat cagaaagcca
     9841 tccaccaact gaaggggctg aaaaagagtc agaagatcct ggatcatatg ggttcaacag
     9901 aactggcggc taatctcttt cgagctaccc aaacagaaga aaaactcaag cgggatagcg
     9961 tgaattcaaa acagcaagct aataccacac actttgacgt gggtagcaag gtgaggcaaa
    10021 ccattcagga acttggcgga accatgcctg aggagttgcc gaccccgcag gtcagcatca
    10081 agcagttgga aaacagtgta aaaattacag agaagaaata gcagaaacga aaaactcttt
    10141 ttgttgattg agacacccga tgcgtaagcc aaggtccagg tgcagtgaac acaatggcta
    10201 aacattgcac ctttcttttc ccccctcagt tttaacctat tttttcttat gcattttctc
    10261 agacaagaag tcagaagaat gcatctacgc tacagaaaat agcgatttca cataactaaa
    10321 agttatcaat ttcctctcac cttaaaaaat atcaatcgtg acaatgcgca caaatcgcta
    10381 ccctgccaga cagattttta gggagagaac catgctgtta cacattttgt atttggttgg
    10441 cattactgcc gaagccatga cgggggcgct ggcggccggg cgacggcgca tggatacatt
    10501 tggcgtaatt attattgcaa ccgccaccgc aattggcgga gggtcagtgc gcgatattct
    10561 gctgggccac tatccgctcg gctgggtcaa acacccggaa tatgtgatta tcgtcgctac
    10621 cgccgcagta ctcaccacta tcgttgctcc tgtaatgcct tacctgcgca aagtgtttct
    10681 ggtgctcgat gcgctcggac tggtggtctt ttctatcatc ggcgcacagg ttgcactgga
    10741 tatggggcac ggcccaatta ttgccgttgt cgcggcggta accaccggtg tatttggcgg
    10801 cgttttgcgc gatatgttct gtaaacgcat tccgctggtc tttcaaaaag agttgtatgc
    10861 cggtgtctca tttgcctccg ccgtgctgta catcgcgctg caacactatg tttctaacca
    10921 tgatgtggtg attatctcca ccctggtatt cggctttttt gctcgtttac tggcgctacg
    10981 tcttaagctg ggattaccgg tcttttacta cagccacgaa ggtcactaag gttcaaaacc
    11041 tgtgatctgc tgggcagcca gccaactgcc cagcttcttg atttgcgcat tttccttcca
    11101 ttcaataacc tgtctggcgc gcccagatcc agtccccggc agctgctgcc agaactgctc
    11161 cgtgctaaat aaaagttgcg accaggatcg ttcatcactg gcattaagcg ccgcccgggt
    11221 tagcggtatt cccattgcca tcacccagcg agtaaaaggc tggttacgag ccagattaaa
    11281 ctgatgccat aactgcgcac ttttactttt cgcgatcccc ggcgtgttct gtaattgctc
    11341 tggcgttaat aaaagccagg aaaagatatg ctcaaagcga tgagtctgat gcagcgcgcg
    11401 ccaaccggcc tcaccaatgc catccagccc cagaacctgt tttgacccca gccagactaa
    11461 gcgtgaaatg aactgttcct gacaaacatc agaagcaaag tagcaggtca acgagttaaa
    11521 gcggttttct ggtggtgtcg gttttgtacg ttctgcaccg cgccagacca catcatcaat
    11581 gcgaggaata ccctgaccgg caaggctgac gaggatctga tcaccaggcg caatatccca
    11641 ctcctgccag cgcctgacgg aaccaatatt cacccgctgg acttttttat catccagcat
    11701 gacaggtgcg agtgacgcaa ccaccgatat tttaccgctc ttacccaccg caaactgaat
    11761 cgccttcact tcggcaacct gagctacagg ttgatatttc caggccacca gccactccgc
    11821 ctggcccggt agccaatgac gggattcggg ttccttagcc cctcgcacaa ccacgccatc
    11881 ggtgacgaag ggtaatttcg ctttccacca ctcattgcgt acgcgcgcaa cctcatcagc
    11941 atttttaacc gcacgggtat acctctgcgt tagagtaaaa cctgcggtag ccagctcctt
    12001 taaacgatcg gtcattaact gcggtccatc cggccatgcc cagacaaaaa cagccagtga
    12061 gtttagcgtg tcgctattcc cctggcgcat catcaggcca gcaacttttg cgcgggcatt
    12121 tattcccccc atttgttgtt ggatatgccc ctcgcgctgg agaaatattt ccccctgaag
    12181 cgtactgttg gctaaaggcc cgctaacggt ttgcggcaca gcggaaatta agctaacttt
    12241 ctgcgtccag tcctcgcctt tcaggccatt accgcgactg attgctttgt tcagtttccc
    12301 gtcccgataa accagggtta ccgcaacgcc atcaactttt ggctgcaccc aaagatcgct
    12361 acgttctcgc atccacagac ttaatgcgtt tttatccgcc attttgcgta cacccgtatg
    12421 ggcaaccgga tgcataaccg caccatttaa aggcggcatc atcacgtcgc ggggctcgct
    12481 cacaaagcag cgttgccact gcgtaagacg ggcgcttaac tgatcgtaaa caccgtcttc
    12541 cacctcactt tttccttcct tccagtagtc atcgtcccac tgttttattt gctgttgcag
    12601 gcgggaaatt tcttcctgtg ctctggctgg cgaccaggcc ggacagaccg cccacacaga
    12661 tgattgccag cacaagatac ttattaatat cgccatccat actttcatct tcacctccgc
    12721 tgtagtcagg ctggcagata tacaacgtgg tgaaggcaaa gccgagtggc aaaaacggag
    12781 tctgcgagga cgcttcctga gaatcgtctt tattgcagta aatcacaggc aaatgcggaa
    12841 acagctacgc aaaacgcaac aactttgcgc aaaaagtgtg agcaagggct acgtcacatg
    12901 gccgcgccgt gtataataag ctcgtatgta ggctttattt cgctaatcac atacgaaaga
    12961 tactcatggc tcaaggcacg ctttatattg tttctgcccc cagtggcgcg ggtaaatcca
    13021 gcctgattca ggctttatta aaaacccaac cgttgtatga cacccaggtt tctgtttcac
    13081 acaccacacg ccaaccgcgt cctggtgaag tccacggtga acattatttc tttgttaatc
    13141 atgatgaatt taaagaaatg attagcagag atgcgttcct cgaacacgca gaagtttttg
    13201 gtaattacta tggcacttcg cgtgaggcca ttgagcaagt actggcgacc ggtgtcgatg
    13261 tttttctcga tatcgactgg cagggcgcgc agcaaattcg ccagaagatg ccgcacgcgc
    13321 ggagtatctt tattttaccg ccgtccaaaa ttgaactgga ccgccgtcta cgcggtcgcg
    13381 gtcaggacag cgaagaggtc attgcaaagc gtatggcgca agctgttgca gaaatgagcc
    13441 attacgccga atatgattat ctgattgtga atgatgactt cgataccgcg ttgaccgatt
    13501 tgaagaccat tattcgcgcc gaacgtctgc gcatgagccg ccaaaagcag cgtcatgacg
    13561 ctttaatcag caaattgttg gcagactgaa cctgatttca gtatcatgcc cagtcatttc
    13621 ttcacctgtg gagcttttta agtatggcac gcgtaactgt tcaggacgct gtagagaaaa
    13681 ttggtaaccg ttttgacctg gtactggtcg ccgcgcgtcg cgctcgtcag atgcaggtag
    13741 gcggaaagga tccgctggta ccggaagaaa acgataaaac cactgtaatc gcgctgcgcg
    13801 aaatcgaaga aggtctgatc aacaaccaga tcctcgacgt tcgcgaacgc caggaacagc
    13861 aagagcagga agccgctgaa ttacaagccg ttaccgctat tgctgaaggt cgtcgttaat
    13921 cacaaagcgg gtcgcccttg tatctgtttg aaagcctgaa tcaactgatt caaacctacc
    13981 tgccggaaga ccaaatcaag cgtctgcggc aggcgtatct cgttgcacgt gatgctcacg
    14041 aggggcaaac acgttcaagc ggtgaaccct atatcacgca cccggtagcg gttgcctgca
    14101 ttctggccga gatgaaactc gactatgaaa cgctgatggc ggcgctgctg catgacgtga
    14161 ttgaagatac tcccgccacc taccaggata tggaacagct ttttggtaaa agcgtcgccg
    14221 agctggtaga gggggtgtcg aaacttgata aactcaagtt ccgcgataag aaagaggcgc
    14281 aggccgaaaa ctttcgcaag atgattatgg cgatggtgca ggatatccgc gtcatcctca
    14341 tcaaacttgc cgaccgtacc cacaacatgc gcacgctggg ctcacttcgc ccggacaaac
    14401 gtcgccgcat cgcccgtgaa actctcgaaa tttacagccc gctggcgcac cgtttaggta
    14461 tccaccacat taaaaccgaa ctcgaagagc tgggttttga ggcgctgtat cccaaccgtt
    14521 atcgcgtaat caaagaagtg gtgaaagccg cgcgcggcaa ccgtaaagag atgatccaaa
    14581 aaatcctctc ggaaatcgaa gggcgtttgc aggaagcggg aattccctgc cgcgtcagtg
    14641 gtcgcgagaa gcatctttat tcgatttact gcaaaatggt gctcaaagag cagcgttttc
    14701 actcaatcat ggacatctac gctttccgcg tgatcgtcaa tgattccgac acctgttatc
    14761 gcgtgctcgg gcaaatgcat agcctgtaca agccgcgtcc gggtcgcgtg aaagactaca
    14821 tcgccattcc aaaagcgaac ggctatcagt ctttgcacac ctcgatgatc ggcccgcacg
    14881 gtgtgccggt tgaggtccag atccgtaccg aagatatgga tcagatggcg gagatgggtg
    14941 ttgccgcgca ctgggcttat aaagagcacg gcgaaaccag caccaccgcg caaatccgcg
    15001 cccagcgctg gatgcaaagc ctgctggagc tgcaacagag cgccggtagt tcgtttgaat
    15061 ttatcgagag cgttaaatcc gatctcttcc cggatgagat ttacgttttc acaccggaag
    15121 ggcgaattgt cgagctgcct gccggtgcaa cgcccgtcga cttcgcttat gcagtgcata
    15181 ccgatatcgg tcatgcctgc gtgggcgcac gcgttgaccg ccagccttac ccgctgtcgc
    15241 agccgcttac cagcggtcaa accgttgaaa tcattaccgc accgggcgct cgcccgaatg
    15301 ccgcgtggct gaactttgtc gttagctcaa aagcgcgcgc caaaattcgt cagttgctga
    15361 aaaacctcaa gcgtgatgat tctgtaagcc tgggccgtcg tctgctcaac catgctttgg
    15421 gtggtagccg taagctgaat gaaatcccgc aggaaaatat tcagcgcgag ctggatcgca
    15481 tgaagctggc aacgcttgac gatctgctgg cagaaatcgg acttggtaac gcaatgagcg
    15541 tggtggtcgc gaaaaatctg caacatgggg acgcctccat tccaccggca acccaaagcc
    15601 acggacatct gcccattaaa ggtgccgatg gcgtgctgat cacctttgcg aaatgctgcc
    15661 gccctattcc tggcgacccg attatcgccc acgtcagccc cggtaaaggt ctggtgatcc
    15721 accatgaatc ctgccgtaat atccgtggct accagaaaga gccagagaag tttatggctg
    15781 tggaatggga taaagagacg gcgcaggagt tcatcaccga aatcaaggtg gagatgttca
    15841 atcatcaggg cgcgctggca aacctgacgg cggcaattaa caccacgact tcgaatattc
    15901 aaagtttgaa tacggaagag aaagatggtc gcgtctacag cgcctttatt cgtctgaccg
    15961 ctcgtgaccg tgtgcatctg gcgaatatca tgcgcaaaat ccgcgtgatg ccagacgtga
    16021 ttaaagtcac ccgaaaccga aattaatgtt ttatgaaccc aacacgttat gcacgcatct
    16081 gcgaaatgct cgccaggcgg cagcctgatc tgaccgtctg catggagcag gtccacaaac
    16141 ctcataacgt ttctgcgatt attcgtaccg cagatgccgt tggcgtacat gaagttcacg
    16201 ccgtctggcc tggtagccgc atgcgcacca tggcttcggc agcggcgggt agtaacagct
    16261 gggtacaggt gaaaacacac cgcaccattg gcgatgccgt cgctcatctc aaaggccagg
    16321 gcatgcagat tctggcaacc catctttctg ataatgctgt cgatttccgc gaaattgatt
    16381 acactcgccc gacctgcatt ttgatgggac aggagaaaac gggcatcacg caggaagcat
    16441 tggccctggc ggatcaggac atcatcattc cgatgatcgg catggtgcag tcgctgaatg
    16501 tttccgttgc ctcagccctc attctttacg aagcccagcg tcagcggcaa aatgcaggca
    16561 tgtacctgcg tgaaaacagc atgttgccgg aagcagagca acaacgcctg ttgtttgaag
    16621 gcggctatcc ggtgctggcg aaagtcgcaa aacgcaaagg cctgccttat ccccacgtca
    16681 atcagcaagg cgagatcgaa gctgatgccg actggtgggc tactatgcag gctgcagggt
    16741 aagtgccatg aaaggtcgcc tgttagatgc tgtcccactc agttccctaa cgggcgttgg
    16801 cgcagcactt agtaacaaac tggcgaaaat caacctgcat accgtgcagg atctactctt
    16861 acaccttccc ctgcgctacg aagatcgcac ccatctctac cccatcggag aactactgcc
    16921 gggcgtttat gccacggtgg aaggcgaagt gctgaactgc aatatctcct tcggcggtcg
    16981 gcggatgatg acctgccaga tcagcgacgg ttccggcatc ctcaccatgc gctttttcaa
    17041 tttcagcgcg gcaatgaaaa atagcctggc gacgggccgc cgtgtactgg cttatggcga
    17101 agcaaagcgc ggtaaatatg gtgcggagat gatccacccg gaataccgcg tgcagggcga
    17161 tctcagcacg ccagaattac aggaaacgct cacgccggtt tatccaacaa cggaaggcgt
    17221 aaagcaggcc acgctgcgta aattaaccga ccaggcgctg gatctgctcg acacctgcgc
    17281 cattgaagaa ctcctgccgc cggaactgtc acaaggaatg atgacgctac cggaagcgtt
    17341 gcgcactttg caccgcccgc caccgacgct acagcttagc gatctggaaa ccgggcagca
    17401 tccggcgcaa cgtcgtctga ttctggaaga actgctggcg cacaacctca gcatgttagc
    17461 cttacgtgcc ggagcacagc gttttcatgc ccagccgctg agcgccaatg acacgctgaa
    17521 aaataaactc ctcgccgcct taccgttcaa gccaacgggc gcacaggcac gcgtagtggc
    17581 ggagatcgag catgatatgg cgctggatgt gccgatgatg cgcctggtac agggcgatgt
    17641 aggttccggt aaaacgctgg tcgccgccct cgccgcgctg cgtgcgattg cccacggcaa
    17701 acaggtggca ctgatggcac caaccgaatt actcgccgag cagcacgcca ataacttccg
    17761 caactggttt gcaccgctcg gtatcgaagt gggctggctc gccggtaagc agaaaggcaa
    17821 agcgcgactg tctcagcagg aagccatcgc cagcggtcag gtgcagatga ttgtcggtac
    17881 acacgccatc ttccaggaac aggtgcagtt taacggcctg gcgctggtga ttatcgacga
    17941 acagcatcgt tttggcgtgc atcagcgtct ggcattgtgg gagaaaggcc agcagcaggg
    18001 cttccatccg catcagttga tcatgaccgc cacgccgatc ccccgcacgc tggcaatgac
    18061 ggcgtatgcc gatctcgata cctcggtgat agatgagctg ccgccaggcc gcacgccagt
    18121 gactacggtc gctattcctg atacccgccg taccgacatc attgaccgcg tgcgccacgc
    18181 ctgcataact gaaggtcgtc aggcgtactg ggtttgtacg ttgattgaag agtcagagtt
    18241 actggaagcg caggcggcgg aagctacctg ggaagagttg aaactggcgc taccagaatt
    18301 gaatgttggc ctggtacacg ggcggatgaa accagccgag aaacaggcgg tgatggcgtc
    18361 gtttaaacaa ggtgagctac atctgctggt tgccactacc gttattgaag tcggtgttga
    18421 tgtgcctaac gccagcctga tgattatcga aaaccctgag cgtctgggtc tggcgcagtt
    18481 acaccagctg cgtgggcgcg taggtcgtgg cgcggtggct tctcactgcg tgctgctcta
    18541 caaaacgccg ctttctaaaa cggcgcaaat tcgcctgcaa gtgctgcgcg acagtaacga
    18601 cggttttgtg attgcgcaaa aagatctgga gattcgcggc cctggcgaat tgttaggcac
    18661 gcgtcagacg ggtaatgctg aatttaaagt ggcggattta ctgcgcgatc aggcgatgat
    18721 cccggaagtt cagcgcctgg cgcgacatat tcacgaacgt tacccacaac aggcaaaagc
    18781 cttgatagaa cgctggatgc cggagacaga acgttactcg aatgcgtaaa aggcggcggt
    18841 gagaagaccg ccgcttcatc ggttaaccgg caaaaatcgg caacattaaa tacagcttaa
    18901 tcaccagcgc attgacgata tcgataaaga acgcaccgac catcggcacc accaaaaacg
    18961 ccatgtgcga cgggccaaag cgttcagtga tcgcctgcat gttggcgatt gccgttggcg
    19021 ttgcaccgag gccaaaacca cagtgacccg cagccagcac tgccgcatcg tagtttttgc
    19081 ccatcatgcg ccaggtaacg aagatggcat acaacgccat gaagatggtc tgtaccacca
    19141 gaatcgccag catcggcagt gccagcgaag ccagctccca cagtctcagc cccatcaacg
    19201 ccatcgccag gaacaacgac aagcttacgt tacccagcac ggataccgca cgctcaaaga
    19261 cgcggtaaaa gcccattatt gacagaccgt tgctcagaat cacgccaaca aacagtacac
    19321 agacgaaggt cggcagttca aaagcagtgc cagccaaaag ttgcgcaaca attttcccca
    19381 ccgtcaggca gatagcaatc agcgcgatag tttcaatcag caccaacgag gtgatcatgc
    19441 gtcccacatc cggcttttca aacgccgtcg ggacttcctg gtcatccgga ataccgttcg
    19501 gcgtggtgga gtgtttcacc agatagcgcg ccaccggacc gccaatcaag ccgcccagca
    19561 ccagaccgaa cgttgcacag gccatcgcca cttccgtcgc attggtgaag ccataacgtt
    19621 caatgaacaa tttactccac gcagcgcccg taccgtgacc gccggaaagc gtaatagaac
    19681 cggccaacag ccccatcagc ggatcaagcc ctaacaggct agccatacca ataccaatgg
    19741 cattttgcat caccaacaga ccaacaacca caatcaagaa gatgccaacc acacgcccac
    19801 cggcacgcaa actggcaatg ttggcgttca ggccaatggt ggcgaagaaa gccagcatta
    19861 acggatcgcg cagggacata tcaaagttga cttcccagcc catgcttttt ttcagtacta
    19921 gtagcgccag cgccaccaac aaaccacccg caacaggttc cggtatggtg tatttcttca
    19981 aaaaggagac ggaatggacc aacttacgcc cgagcagcaa cgtcagcgtt gcggcaacaa
    20041 gcgttgctaa agtatcgaga tgaaacatag ttactccttt gtatccgcat cactctttca
    20101 tactcgtcat acttcatcgc gcagtggcaa cttcatgaac tatgacgggt atcgagttat
    20161 gctttcctgg gtcaaattct cagctgttaa tgaacaaatg gcatggattt taagcagaaa
    20221 aataccaaaa gttatataaa aaagtgcatt tatctcattt aaatatttag atgaggatgt
    20281 ggcaaacgtt tgcttttctc tttcggtcag ctaaaatgcc cgctttgcta ccacgggatt
    20341 gttttcaatg tctgtttcca ccctcgagtc agaaaatgcg caaccggttg cgcagactca
    20401 aaacagcgaa ctgatttacc gtcttgaaga tcgtccgccg cttcctcaaa ccctgtttgc
    20461 cgcctgtcag catctgctgg cgatgttcgt tgcggtgatc acgccagcgc tattaatctg
    20521 ccaggcgctg ggtttaccgg cacaagacac gcaacacatt attagtatgt cgctgtttgc
    20581 ctccggtgtg gcatcgatta ttcaaattaa ggcctggggt ccggttggct ccgggctgtt
    20641 gtctattcag ggcaccagct tcaactttgt tgccccgctg attatgggcg gtaccgcgct
    20701 gaaaaccggt ggtgctgatg ttcctaccat gatggcggct ttgttcggca cgttgatgct
    20761 ggcaagttgc accgagatgg tgatctcccg cgttctgcat ctggcgcgcc gcattattac
    20821 gccgctggtt tctggcgttg tggtgatgat tatcggcctg tcgctaattc aggtggggct
    20881 gacctccatt ggcggcggtt acgcagccat gagcgataac accttcggcg caccgaaaaa
    20941 tctgctgctg gcaggcgtgg ttttagcctt aattatcctg cttaaccgtc aacgtaaccc
    21001 ttacttacgc gtggcctcac tggtaattgc gatggcggcc ggatatgcgc tggcgtggtt
    21061 tatgggcatg ttgccagaaa gcaacgaacc gatgacgcaa gaactgatta tggtgccaac
    21121 gccgctctat tacggtcttg gcattgaatg gagtctgctg ctgccgctga tgctggtctt
    21181 tatgatcact tcgctggaaa ccattggcga tatcacggcg acctctgacg tttccgaaca
    21241 gccagtgtcc ggtccgctgt acatgaaacg cctgaaaggc ggcgtgctgg caaacggcct
    21301 gaactcgttt gtttcggcgg tgtttaacac cttcccgaac tcctgcttcg ggcaaaacaa
    21361 cggagtgatc cagttgactg gtgttgccag ccgctatgtc ggttttgtcg tcgcgctgat
    21421 gttgatcgtg ctgggtctgt tcccggcagt gagcgatttt gtacaacaca ttccagaacc
    21481 ggttctgggc ggcgcaacgc ttgtaatgtt tggcaccatc gccgcctccg gtgtgcgtat
    21541 cgtttctcgt gagccgctga accgtcgggc gattctgatt atcgcgctgt cgctggcggt
    21601 tggtctgggc gtgtctcagc agccgctgat tttgcagttt gcccctgaat ggctgaaaaa
    21661 cctgctctcc tccgggatcg ccgcgggcgg tattactgcc atcgtgctga atctgatttt
    21721 cccaccagaa aaacagtaat tcactggcgg cggtaagata attaccgccg cagtctcacc
    21781 tctttcacca ttcccgcctt gaggattgcg cgtaaatcgt gcataactcc cttatgtgca
    21841 tttcacggga tggaagacca tgaaatttat tgggaagctg cttctctaca ttctcatcgc
    21901 tctgttagtg gtgatcgctg gcctctattt tcttctgcaa acccgctggg gagcagaaca
    21961 tatcagcgca tgggtttccg agaatagcga ctatcatctg gccttcgggg cgatggatca
    22021 ccgtttttcc gcgccatctc atatcgtgct ggagaatgtc acgtttggtc gtgatggtca
    22081 gcccgcgccc ctggtggcaa aaagtgtcga cattgcgcta agcagtcgcc aactgaccga
    22141 accacgccat gtcgatacca tcctgctgga aaacgggacg ctgaatctca ccgaccagac
    22201 cgcgccgcta ccgttcaaag ccgatcgtct gcaactgcgt gatatggcgt ttaatagccc
    22261 gaatagagaa tggaaactga gcgcgcagcg ggtaaatggc ggcgtggttc cgtggtcacc
    22321 agaagccggt aaagtgctgg gtacgaaggc gcagattcag tttagtgccg gatcgctttc
    22381 gctcaatgat gttcctgcca ccaatgtact gattgaaggc agtattgata acgatcgcgt
    22441 tacgctgact aacctgggtg ccgacatcgc ccgcgggaca ttaaccggaa acgcgcagcg
    22501 taacgccgac ggcagctggc aagtggaaaa tctgcgcatg gcggatatcc gtctacaaag
    22561 cgaaaaatcg ctaaccgact tctttgcgcc attacgctct gtcccgtcgt tgcagattgg
    22621 tcgcttggaa gtcatcgatg ctcgtctgca aggtccggac tgggcggtga ccgacctcga
    22681 tctcagcttg cgcaacatga ccttcagtaa agatgactgg cagacacagg aaggcaaact
    22741 gtcgatgaac gccagcgagt tcatttatgg ttcgctgcat ttatttgacc cgattataaa
    22801 cgcggaattt tccccgcagg gcgtagcgct gcgccagttc accagccgct gggaaggggg
    22861 tatggtcaga acgtcaggga actggctgcg tgacggtaaa acgttgatcc ttgatgatgc
    22921 ggcaattgcc gggctggaat ataccttgcc gaaaaactgg caacagttgt ggatggaaac
    22981 gacacccggt tggttaaaca gcctgcaact gaagagattt agcgccagcc gcaatctgat
    23041 cattgatatc gaccctgact tcccgtggca gctcaccgcg ctcgatggtt acggtgccaa
    23101 cctgacgctg gttaccgatc ataaatgggg cgtctggagt ggctcggcga atctgaatgc
    23161 cgccgccgcg acattcaatc gtgttgatgt tcgtcgcccg tcgctggcgc tgaccgccaa
    23221 cagcagcacg gtgaatatca gcgaactgag tgcatttact gaaaaaggca ttctggaagc
    23281 caccgcaagt gtttcacaaa cgccacaacg tcagacccat atcagcctga atggacgcgg
    23341 tgtgccggtg aatattttgc aacaatgggg atggcctgaa ttacagttga ctggcgacgg
    23401 caatattcag cttaccgcca gtggagatat tcaggccaat gccccgctga aacctacggt
    23461 tagcgggcaa ttgcatgccg tgaacgccgc aaagcagcaa gtgactcaaa ccatgaatgc
    23521 gggcgtcgtt tccagcggtg aagttacatc gacggagccg gtgcggtaaa catcaatctc
    23581 gccgggtacg actcagacgt acccggcatt ccgtcaatag attacaacgt aattgtcagc
    23641 gcattccctt gaggcgtcac caccagcccc tgctcacttt cagcctgcga accgccttgc
    23701 agaccattta ctttcacaac attgcgcaag cacagcgtcc agttcttcgc ctcgcccgca
    23761 ccagtcacag taatcgtgtt gccagtacgt gctgctttta aagtaaagat caccgatccg
    23821 tcagcagcag gcacttcaca gacggcttca tgcccgtctt gcagattaaa gaggtggaat
    23881 gccgtgcctt cgtgccacac gtaatcggga cgttgatcgt tgttgcccag cgccagtaga
    23941 gtgttatcac gcacataaac gggcagactc aggaagccgt gctgctgttt atgccagcga
    24001 ctaccgtcga gttcatcgtt gtgccacagg tgtgtccagc gaccttccgg caggtagaac
    24061 tgcacatcgc ccgcttcagt gaacaccggc gcaaccatca cgttgtcgcc taacatgtat
    24121 tgacggtcaa ggtaatcaca agccggatcg tccgggaact ccatcatcat ggcccgcatc
    24181 atcggcgtac cccgcgcgtt cgcacgcgca gcttcacgat acagatacgg catcatgcgg
    24241 catttcagtt gcgtgaagaa gcgcaccaca tcacaggact catcatcgta ggcccacggc
    24301 acacgataag atttgctacc gtgtaaacgg ctatggctgg agagcaaacc aaacgcgcac
    24361 cagcgtttgt aaacgtgcgc cggagcggta ttttcaaagc cgccgatatc gtggctccag
    24421 aagccaaaac ctgaaaggcc aatagacaaa ccaccgcgca ggctttccgc cattgattcg
    24481 tagttagcgt aacaatcgcc accccagtgt accgggaatt tctgcgcacc gacggaggcc
    24541 gagcgggcaa acaagacagc ttcttcctca ccaacggtgt ccttgagcac gttccacacc
    24601 agttcgttgt agatgtacgc ataatggtta tgcattttct gcggatcgga accgtcaaac
    24661 cactgaacat cagttgggat acgttcgcca aagtcggtct taaagcaatc aacgcccatc
    24721 gcgaccagac ctttcagttt gtcggcgtac catttgcagg catccggatt ggtaaagtca
    24781 taaatcgcca gacctggctg ccatttatcc cactgccata gcgaaccgtc cgggcgtttg
    24841 agtaaatagc ctttctcttg taactcttta aagacggggg atttttgacc gatatagggg
    24901 ttaatccaga cgcagatttt cagccctttc gctttcaggc ggcggatcat cccttccggg
    24961 tcagggaaag tcagcgggtc ccactcaaaa tcgcaccact ggaaggcttt catccagaaa
    25021 cagtcaaagt ggaaaacatg cagcggcaga ttgcgttccg ccataccatc gataaagctg
    25081 tttaccgtcg cttcgtcgta gttggtggta aatgaagtgg ttagccacag gccgaaggac
    25141 cacgggggcg gcagcgccgg acgaccagta aagcgggtat aacgatcaag taccgctttc
    25201 ggcgtcgggc cgtcgataac aaagtattcg agatattcac tctcaacgct gaactgcact
    25261 ttggagactt tctccgatcc cacttcaaaa gagacacact ggggatgatt gaccagtacc
    25321 ccataaccac ggttagtcat gtagaacggg atatttttat acgcctgttc agtacttgtg
    25381 ccgccgtccc ggttccaggt ctctaccgtc tggccattgc gcaccagggc agtaaagcgc
    25441 tctcccagac cgtaaactgt ttcgccaacg ccaagatcaa gccgctcaaa catataattg
    25501 cgttgattat tcgtgtcctg cacgtagcca ttatttttca cctgactacc ggtaatacgt
    25561 tcgccgttgc gcagaaaatc cagtgaccag aactcacctt tgctgacacg cgcgcttaag
    25621 ttgccacttt taaactcagc ataacgttct gtgttttcga ttgtgacctt cacgtcctgc
    25681 aaaatattga gcggataatg aggaccgtta ttcagcgccc cctgaaaata ctcaatccgc
    25741 acaccgacaa taccttcctg tggggagaaa aagcgcagcg taaataaagg cgtatcaagc
    25801 tgccaggtac gttcacgcac atcacggggg gcagcataga ccaccatttc attatcctgc
    25861 tgttcaacct cgaatacctg aagcgggtga atcaaattga ggccaggttg aatcaaccag
    25921 tttccatcgc taattttcat tgccgttcct tagttctgca cttcttgaga ggtgaattgt
    25981 tgctggcaac ggcgtttacc ctgagccagc tgttccataa cggttttcag attgtgcgtc
    26041 gtgagtgagt agtagcgttt agcgataatc gcgctcagca aataacagat cgccggaaca
    26101 atcgtgaata gcgcaataat gatgctaatc gtggcgctgt tctgcgcttt ttctgccgca
    26161 tcatatccgc cataagccag catccagccg ataagagcgc cgccgaaggc caaccccagt
    26221 ttgagcacaa acagcgtgcc agcaaaactg atcccggtca ggcgtttacc attgcaccac
    26281 tcgccgtagt cgacggtatc gaacatcatt acccactgga taggtgtcac cagttgatgc
    26341 aacacaccaa tcacgaagat gaagacaaac atagtgatgc tggcctgcat gggaacaaag
    26401 aacatcgcga ggctaatcac tgccagcagg gcgttcgtcc accagaagat agtgacttta
    26461 catttccagt cggtcagagg ttttgccagt gcggaaccaa tcaggttacc cacgcaataa
    26521 gtggtgagaa aagcgacaaa cacttccggc gtgcccaaaa tccatgtgac gtaatacatc
    26581 atcgccccac cgcgtacgca caccgccagg atattgaaaa tggttagtaa accgacaatc
    26641 cgccactggt cgttttgcca gatatcacgt aaatcttccc gcatagacgt tgttgtaggt
    26701 ggtgcttcaa cgcgttcttt agtggtgaag aaacaaaatg ccagcatcat gaatgccacc
    26761 acggaaagga ccacgatacc gccctggaaa ccgagtggtt tattatcacc gccaattaaa
    26821 ttaaccagtg gcatcatcag aacagtagaa agcatgcctc ccgcggtcgc cagcacaaaa
    26881 cgccaggatt gcagcgagat acgctgagtc gggtcattgg tgattacacc acccaatgcg
    26941 cagtaaggga tattgacgac ggtataaagt aaggtaagtt agggtgtaag taattgctgc
    27001 atagatcatt ttgccgttca tactgagatc tggcgtgcta taggccagta cacagacgat
    27061 cccgaatggc agtgcgccaa acagtaccca cggacgaaat ttaccccagc gagagcgcgt
    27121 tcgatcggcc aacaacccca tgcaaggatc ggaaatcgca tccagtgcac gagcgaccaa
    27181 aaacatggtt ccgacaaatc cggcaggaat gccaaaaata tcggtataaa agaacatcat
    27241 atataacatt acgttatcga aaataatgtg gctggcggcg tctcccatgc cataaccaat
    27301 tttctcttta acggacaaca cttcactctt catctgcttt tccttgatgc tataggggtg
    27361 ctgagaccga taacgcattt tgtaaaccat ccctgtgaga gcgaatattc cgatatctgg
    27421 ttattaattt atgattcttg ttttatgtga tcgtggtagc gttaattccg ctcatatatc
    27481 attgtaaaat atgggtttta tataaattat aatgctttcg tgataatacg ctgcgtttat
    27541 taggcggaaa aaactgatct gggggatgta gaaactcaag gaagtagcta taatgcgccc
    27601 cgcctccatg tagcaatcga ggcgcggaag atcgtcgtct ccggtgaggc ggctggactt
    27661 caaatccagt tggggccgcc agcggtcccg ggcaggttcg actcctgtga tcttccgcca
    27721 gtttacctct tctgaactct cccaatgtca ataaatctct tctaatccct tgtatatcag
    27781 tatttatttg tatcctgagg tcaacctact tcaccccgta tcaagtttct tttgggggta
    27841 ctttaggggg tgttatttcc tattgaaata caagcacccc cataatagga aaaatgtgat
    27901 acataacaac ggcttttaac aatatctttg ttatacaagg ggtgtattgg gggtatcagg
    27961 ttagttactg gcagaaatcc ccctcaggaa catgaattca tcaaaagccg gaggatgtca
    28021 tggcattaac cgatatcaaa gtgaaaaccg caaaacccaa agacaaaccc tataagcttg
    28081 ctgatggcgg cggtatgtat ctactgatta acactaatgg ttctaaatac tggcgcatga
    28141 agtaccgctt tgctggtaaa gaaaagatgc tttctattgg cgtatacccc gatgtaacgc
    28201 tagccgatgc tcgcgagaaa cgcagtgaag ccaggaaact cctcgctgct ggaggcgatc
    28261 caggggaggc aaaaaaggaa gaaaaaatcg cgcagcaaat gagtctgaaa aatacgttcg
    28321 aggctattgc tcgcgaatgg caccagttaa aagctgatcg gtggtcatta cgctatcgtg
    28381 atgaaatcat tgataccttt gaaaaagaca tttttcccta cattggcaag cgccctattg
    28441 ccgaaatcaa accaatggag ttactggaag cactgcgtaa aatggaaaaa cgtggagctc
    28501 tggagaaaat gcgcaaggtc cgccagcgct gtggtgaggt gttccgctat gcaattgtta
    28561 ctggccgggc agactataac ccagcccccg atcttgccag tgctttagct acaccgaaaa
    28621 aagtacattt tccctttctt actgctaatg aacttcccca cttcctcaac gatctggcgg
    28681 gttataccgg aagtataatc actaagacag ctactcagat cattatgtta accggcgtac
    28741 gaacacaaga actgcgtttt gcacgctggg aagatatcga ttttgagaca aaattgtggg
    28801 agatcccggc agaagtaatg aagatgaagc gacctcatat tgtgccgctc tctgagcagg
    28861 tcattatgct atttaagcag cttgaaccaa tctcaaaaca tcatccccta gtctttatcg
    28921 gtagaaatga tccccgcaaa ccaattagta aggaaagcat taaccaagta attgaattac
    28981 ttggatataa gggaagactc acagggcacg gtttcagaca caccatgagt acaattctgc
    29041 atgaacaagg ctttaattcc gcctggattg aaatgcagtt agctcatg
//
LOCUS       DAETPZ010000046        28463 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.046, whole genome shotgun sequence.
ACCESSION   DAETPZ010000046 DAETPZ010000000
VERSION     DAETPZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.046"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..1198
                     /gene="pdeC"
                     /locus_tag="K0W59_002301"
     CDS             <1..1198
                     /gene="pdeC"
                     /locus_tag="K0W59_002301"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HBI3023311.1"
                     /translation="IYYYRDTPFFSGYKMTYMQRGNYVAVINPLFWSEVMSDDPTLQW
                     GVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATIYSTKRPIAAIVATSY
                     QRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQRALEKHQLCLYYQP
                     IIDIKTEKCIGAEALLRWPGEQGQIMNPAEFIPLAEKEGMIEQITDYVIDNVFRDLGA
                     YLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKFEVTEHAFLDVEKMT
                     PIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIAEH
                     IIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMPPQVFMQWMEQLPAR
                     ELTRGQ"
     gene            complement(1201..1524)
                     /gene="soxS"
                     /locus_tag="K0W59_002302"
     CDS             complement(1201..1524)
                     /gene="soxS"
                     /locus_tag="K0W59_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HBI3023312.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1610..2074
                     /gene="soxR"
                     /locus_tag="K0W59_002303"
     CDS             1610..2074
                     /gene="soxR"
                     /locus_tag="K0W59_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HBI3023313.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2778..4127
                     /gene="ghxP"
                     /locus_tag="K0W59_002304"
                     /gene_synonym="yjcD"
     CDS             2778..4127
                     /gene="ghxP"
                     /locus_tag="K0W59_002304"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HBI3023314.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4278..5927
                     /locus_tag="K0W59_002305"
     CDS             4278..5927
                     /locus_tag="K0W59_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HBI3023315.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(6081..7373)
                     /locus_tag="K0W59_002306"
     CDS             complement(6081..7373)
                     /locus_tag="K0W59_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBI3023316.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(7551..9200)
                     /gene="actP"
                     /locus_tag="K0W59_002307"
     CDS             complement(7551..9200)
                     /gene="actP"
                     /locus_tag="K0W59_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HBI3023317.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLITERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVMLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIISFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(9197..9511)
                     /locus_tag="K0W59_002308"
     CDS             complement(9197..9511)
                     /locus_tag="K0W59_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HBI3023318.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(9711..11669)
                     /gene="acs"
                     /locus_tag="K0W59_002309"
     CDS             complement(9711..11669)
                     /gene="acs"
                     /locus_tag="K0W59_002309"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HBI3023319.1"
                     /translation="MSQIHKHTIPANITDRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPKAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            12062..13498
                     /gene="nrfA"
                     /locus_tag="K0W59_002310"
     CDS             12062..13498
                     /gene="nrfA"
                     /locus_tag="K0W59_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HBI3023320.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALEEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAVLDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            13543..14109
                     /gene="nrfB"
                     /locus_tag="K0W59_002311"
     CDS             13543..14109
                     /gene="nrfB"
                     /locus_tag="K0W59_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HBI3023321.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            14106..14777
                     /gene="nrfC"
                     /locus_tag="K0W59_002312"
     CDS             14106..14777
                     /gene="nrfC"
                     /locus_tag="K0W59_002312"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HBI3023322.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCEHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            14774..15730
                     /gene="nrfD"
                     /locus_tag="K0W59_002313"
     CDS             14774..15730
                     /gene="nrfD"
                     /locus_tag="K0W59_002313"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HBI3023323.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEYDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAVCGASLVGVLMLRFFILYAGQLTVA"
     gene            15750..17468
                     /locus_tag="K0W59_002314"
     CDS             15750..17468
                     /locus_tag="K0W59_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HBI3023324.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLAAFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            17461..17844
                     /gene="nrfF"
                     /locus_tag="K0W59_002315"
     CDS             17461..17844
                     /gene="nrfF"
                     /locus_tag="K0W59_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HBI3023325.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            17841..18437
                     /gene="nrfG"
                     /locus_tag="K0W59_002316"
     CDS             17841..18437
                     /gene="nrfG"
                     /locus_tag="K0W59_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HBI3023326.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            18779..20092
                     /gene="gltP"
                     /locus_tag="K0W59_002317"
     CDS             18779..20092
                     /gene="gltP"
                     /locus_tag="K0W59_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HBI3023327.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWSLIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(20170..20859)
                     /locus_tag="K0W59_002318"
     CDS             complement(20170..20859)
                     /locus_tag="K0W59_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBI3023328.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDQRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(20953..23100)
                     /gene="fdhF"
                     /locus_tag="K0W59_002319"
                     /pseudo
     CDS             complement(20953..23100)
                     /gene="fdhF"
                     /locus_tag="K0W59_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(23298..24764)
                     /gene="mdtP"
                     /locus_tag="K0W59_002320"
     CDS             complement(23298..24764)
                     /gene="mdtP"
                     /locus_tag="K0W59_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HBI3023329.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(24761..26812)
                     /gene="mdtO"
                     /locus_tag="K0W59_002321"
                     /pseudo
     CDS             complement(24761..26812)
                     /gene="mdtO"
                     /locus_tag="K0W59_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
     gene            complement(26812..27843)
                     /gene="mdtN"
                     /locus_tag="K0W59_002322"
     CDS             complement(26812..27843)
                     /gene="mdtN"
                     /locus_tag="K0W59_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HBI3023330.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGDDSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAALEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(27862..28137)
                     /locus_tag="K0W59_002323"
     CDS             complement(27862..28137)
                     /locus_tag="K0W59_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023331.1"
                     /translation="MPTVLSRMAIHLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(28346..>28463)
                     /locus_tag="K0W59_002324"
     CDS             complement(28346..>28463)
                     /locus_tag="K0W59_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023332.1"
                     /translation="ADLVKAKRAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         6485 a         7207 c         7641 g         7130 t
ORIGIN      
        1 tatctattat taccgcgata cgcctttttt ctctggctat aaaatgacct atatgcagcg
       61 gggaaattat gtggcagtta tcaaccctct cttctggagt gaagtgatgt ctgatgaccc
      121 gacattgcaa tggggtgtgt atgatacggt gacgaaaacc tttttctcgt taagcaaaga
      181 ggcctcggca gcaacgtttt ctccgctgat tcatttgaag gatttaaccg tacaaagaaa
      241 tggctattta tatgcgacaa tttattcgac aaaacgccca attgcagcca ttgttgcgac
      301 ttcatatcaa cgtcttataa cccattttta taatcatctt atttttgcgt tgcccgccgg
      361 tattttgggg agtcttgttc tgctattact ctggctacgt attcgacaaa actatttatc
      421 tcccaaacgt aaattgcaac gcgccctcga aaaacatcaa ctttgtcttt attaccagcc
      481 aataatcgat atcaaaacag aaaaatgtat cggcgctgaa gcgttgttac gttggcctgg
      541 tgagcagggg caaataatga atccggcaga gtttattccg ctggcagaaa aggaggggat
      601 gatagaacag ataactgatt atgttattga taatgtcttc cgcgatctgg gcgcgtacct
      661 ggcaacacat gccgatcgct atgtttctat taacctgtcg gcctccgatt ttcatacgtc
      721 acggttgata gcgcgaatca atcagaaaac agagcaatac gcggtgcgtc cgcagcaaat
      781 taaatttgaa gtgactgagc atgcatttct tgatgtcgaa aaaatgacgc cgattattct
      841 ggctttccgc caggctggtt acgaagtggc aattgatgat tttggtattg gctactctaa
      901 cttgcataac cttaaatcat tgaatgtcga tattttgaaa atcgataaat cgtttgttga
      961 aacgctgacc acccacaaaa ccagtcattt gattgcggaa cacatcatcg agctggcgca
     1021 cagcctgggg ttaaaaacga tcgctgaagg cgtcgaaact gaggagcagg ttaactggct
     1081 gcgcaaacgc ggcgtgcgct attgccaggg atggtttttt gcgaaggcga tgccgccgca
     1141 ggtgtttatg caatggatgg agcaattacc cgcgcgggag ttaacgcgcg ggcaataaaa
     1201 ctacaggcgg tggcgataat cgctgggagt gcgatcaaac tgccgacgga aaacgcggga
     1261 gaaggtctgc tgcgagacat aacccaggtc cattgcgata tcaaaaatcg gacgctcggt
     1321 ggtgcgcaac tcaacggcgg ccagtaacag gcggcgttgg cgaatgtaat cgccaagcgt
     1381 ctgatgcgtc accgtgcgga acatccgttg caagtaccac tttgaatagc ctgatttttt
     1441 tgcgacgaca tcaatgttaa gcggctggtc aatatgcacg tcaatccatg cgataagatc
     1501 ctgaataatt ttctgatggg acataaatct gcctcttttc agtgttcagt tcgttaattc
     1561 atctgttggg gagtataatt cctcaagtta acttgaggta aagcgattta tggaaaagaa
     1621 attaccccgc attaaagcgc tgctaacccc tggcgaagtg gcgaaacgca gcggtgtggc
     1681 ggtatcggcg ctgcatttct atgaaagcaa agggttgatt actagtatcc gtaacagcgg
     1741 caatcagcgg cgatataaac gtgatgtgtt gcgatatgtc gcaattatca aaatcgctca
     1801 gcgtattggc attccgctgg cgaccattgg tgaagcgttt ggcgtgttgc cagaagggca
     1861 tacgttaagt gcgaaagagt ggaaacagct ttcgtcccaa tggcgagaag agttggatcg
     1921 gcgcattcat accttagtgg cgctgcgtga cgaactggac ggatgtattg gttgtggctg
     1981 cctttcgcgc agtgattgcc cgttgcgtaa cccgggcgac cgcttaggag aagaaggtac
     2041 cggcgcacgc ttgctggaag atgaacaaaa ctaaagcgcc acaaggacaa gctgactcat
     2101 cgttaactat gaggccgggg tgagggtaaa tatttgcact ggtgctgcct gttcccctca
     2161 ccctaaccct ctccccgaag gggcgagggg actgatcgag ctcttttgga attatccgca
     2221 aacacaaaac taaagcgcca caagggcgct ttggtttgtt ttccggtctt tgtctttctc
     2281 tctatcccgc tggaacacag gagggtttcc cccgacgtca acacacctca ttcgagcacg
     2341 tggtggaggt tccggttggt gttgatgctt taattgtatg tcaccgacgt ttcttcgcca
     2401 gtgtaaaagt atacttttta gccgcaatat ttttgtcatc tcagacgatt ttttatcgca
     2461 atccagaacg gtatacggct cgataacgct gcaatcttgc acatcgacga taacgtttgc
     2521 gcatcaattg cctggttttt catcgtcaag acaataaaag agaaaaaagc agcaaacttc
     2581 ggttgaaaaa gccgctatga tcgccggata atcgtttgct tttttaccac ccgttttgta
     2641 tgcgcggagc taaacgtttg cttttttgcg acgcaccaaa ttgccgcaaa cctagagcag
     2701 gaagataacg tttcgctggc aggggattgt ccgccacgca tcttgacgaa aattaaactc
     2761 tcaggggatg ttttcttatg tctacgccat cagcgcgtac cggcggttca ctcgacgcct
     2821 ggtttaaaat ttcacaacgt ggaagcactg tccgtcagga agtggttgcc gggttaacaa
     2881 cgtttctggc gatggtctac tcggtcatcg tcgttccagg tatgttgggt aaagcaggct
     2941 tcccgcctgc ggcagttttc gttgcaacct gtctggttgc cggactcggt tctatcgtga
     3001 tgggtctgtg ggctaatctg ccgttggcga ttggttgcgc catctccctg acggcgttta
     3061 ccgcattcag cctggtgctg gggcaacata ttagcgtacc tgtcgcgctg ggtgccgtgt
     3121 tcctgatggg ggtgctgttt acggtaattt ctgccacggg tatccgtagc tggattttgc
     3181 gcaacttgcc gcacggtgtg gcgcacggca cggggatcgg tatcggtctg ttcctgctgc
     3241 tcattgccgc taatggtgtc ggtctggtga ttaaaaaccc gcttgatggt ctgcccgttg
     3301 cgctgggtga tttcgcgacc ttcccggtga ttatgtcact ggtaggtctg gcggtgatca
     3361 tcggcttgga aaaactgaaa gtccctggtg gcattctgct gaccattatc ggtatctcaa
     3421 ttgtcggttt gatcttcgat cctaacgtcc atttctccgg cgttttcgcc atgccttcat
     3481 tgagcgatga aaacggcaat tcactgattg gcagcctgga cattatgggc gcgctgaatc
     3541 ctgtagtcct gccaagcgtt ctggcgctgg tgatgacggc agtatttgat gccaccggaa
     3601 ctatccgcgc tgtcgccggc caggcgaacc tgctggataa agatgggcag attatcgacg
     3661 gcgggaaagc actgaccact gactccatga gcagcgtttt ctctggcctg gtgggtgcag
     3721 ctccggcagc ggtatacatc gagtctgcgg cgggtacggc ggcgggcggt aaaaccgggt
     3781 tgacggctat caccgttggc gtgctgttcc tgctgattct gttcctctct ccgctttctt
     3841 acctcgttcc ggggtatgca acggctccgg cgctgatgta cgttggcctg ctgatgctga
     3901 gcaacgtggc gaaaatcgac tttgctgatt ttgttgatgc gatggcgggt ctggttacgg
     3961 cggtattcat cgtgctgacc tgtaacatcg taacaggcat catgatcggc ttcgcgactc
     4021 tggtgattgg tcgtctggtt tccggtgaat ggcgcaagtt gaacatcggt acggtcgtta
     4081 tcgccgtggc gctggtgacc ttctatgcgg gtggctgggc tatctaatct ttcatcggat
     4141 tctgaaaacg ggtggcaatg gctgcccgtt tttattttct ccgcacattg tgtgagcttt
     4201 tgcgatattc tgaaaaaatg agaattcagg cataacgtct ttctcagatc ataaaaaaca
     4261 tcgcaaacag ggaacgcatg gaaatcttct tcaccatact gataatgacc ctcgtggtct
     4321 cgctgtccgg ggtggtcact cgtgtcatgc cctttcagat cccgcttccg cttatgcaaa
     4381 tcgccatcgg tgcgctactg gcgtggccga cgtttggttt gcatgtggag tttgatcctg
     4441 aactcttttt agtcttgttt atcccgccgt tgctgttcgc tgatggctgg aaaacgccga
     4501 cccgtgaatt tctcgaacat ggtcgagaga ttttcggcct cgcgctggcg ctggtggtgg
     4561 tcaccgtggt cggcattggc ttccttattt actgggtggt gccgggcatt ccgctgatcc
     4621 ccgcctttgc gctggcggcg gtactttctc cgaccgatgc tgtggcgctc tccgggattg
     4681 ttggcgaagg gcgcatcccg aaaaaaatca tgggcatttt gcagggcgaa gcgttgatga
     4741 atgacgcttc cggcctggtg tcgttgaagt ttgccgtggc agtggcgatg gggacgatga
     4801 tcttcactgt cggcggtgcg acggtcgaat ttatgaaagt agccattggc ggtattcttg
     4861 ccggttttgt ggtgagctgg ctgtatggtc gctcgctgcg attcctcagc cgctggggcg
     4921 gtgatgaacc cgcgacgcag atcgttctgc tgttcttgct gccattcgct tcttatctga
     4981 ttgccgaaca tattggcgtt tcgggcatcc tcgctgcggt tgccgccggg atgaccatca
     5041 cccgctccgg tgtgatgcgc cgtgcgccac tggcaatgcg cctgcgcgca aacagcacct
     5101 gggcgatgct ggaatttgtc tttaacggca tggtgttcct gctgttaggt ctgcaactgc
     5161 cgggtattct ggagacgtcg ctgatggcgg cagaaatcga ccctaacgtc gaaatctgga
     5221 tgctgtttac cgatattatt ctgatatatg cggcgctgat gctggtccgt ttcggctggc
     5281 tgtggacgat gaaaaagttc agcaaccgct tcctgaagaa gaagccgatg gagtttggtt
     5341 cgtggaccac acgagaaatc ctgatcgcgt ctttcgctgg ggtgcgtggg gcgatcactc
     5401 tggccggtgt gctctcaatc ccgctgctct tgccggatgg taacgtcttc ccggcgcgct
     5461 atgagctggt gttcctggcg gctggcgtca ttctcttctc gctgtttgtc ggcgtggtga
     5521 tgttgcctat tctgctacaa cacattgaag tcgcggacca ttcgcaacaa ttgaaagaag
     5581 aacgtattgc gcgagcggca acggcagaag tggcgattgt ggcgatccag aaaatggagg
     5641 agcgtctggc ggcggatacc gaagagaata tcgataacca gttgcttacg gaggtcagtt
     5701 ctcgcgtcat tggtaacctg cgtcgtcgcg ccgatggacg taacgacgtt gaaagttccg
     5761 tgcaggaaga gaaccttgag cgtcgcttcc gtctggcggc attgcgttct gaacgtgctg
     5821 aactgtacca cctgcgcgcc acgcgggaga tcagcaacga aacgctgcaa aaattactgc
     5881 acgatctcga tttgcttgaa gcgttgctaa ttgaggaaaa tcagtaagcc gaaagcgacg
     5941 taaccaggcc cgaggtaatc tgaaaaaggt gcaatctttt gcacctttaa caggttagct
     6001 cgggcgaaca ttcatgattc taaaacaaat aagttatctc tcttgtaaag tcaattcatt
     6061 ggctgttctt cgacttaacc tcaatctgcg tgttgcataa tatctgcagt aatcttcata
     6121 gcgactgcat taatattatc ctcagtttgt gacatgccag tatgagaggt acaatcttct
     6181 tcaaagaatt tctgtagtgt taccagggta tcactatcaa tggggatatt ttcctcccca
     6241 cggagaatgg aaatgcccat tccctggacg ctagcctcat cgaaattaac ccgatagaga
     6301 cgggtattaa acatacaggc ttttgttaaa atagaacagt tgaaatggca gttgcttaaa
     6361 ttggcattat taaaattaac attagccata ttagagccgg tacatataac catttcgagg
     6421 aatgtatttg tcagccacgt acaaaagagt ttgctatcaa ttaagtttat attctggagg
     6481 attgctttgt tcagattgac gcgagacaga tcagcatgtt caaagttaat taaatcgagt
     6541 ttagagaaac taaggtcggc tttaaataaa ttaacatatg gcgctattac tttataaaga
     6601 tttacctcag ccatgtacgc catataaaaa tttgaatagt caaggaaggt atttttaata
     6661 ttggctctat tcattgctgc attgtagaat tgaacatgca tagcggagca tgcatttaga
     6721 gttccgtcgg ccatttttat ctgagcgaaa tttgagtcat cgagaataca attatcaaaa
     6781 agtatttttt ccattatggc gtaataaaaa ttcgttttat acatattaca gtcctggaaa
     6841 atagcgtttt gtagtctgca acctgcaaaa ttcgaattgg ataaatcttc atctttaatc
     6901 acgataccct tcaggctaag gtgtgaaaaa tcgtaaccac catttactat ctgtggaatt
     6961 tcgatagtat tattatggac ggcgacatta tttataaaat ataccacctt aatggataca
     7021 tccttttctt tactaaggta aactcttctc gtaattctat cgtcacattt attatttgca
     7081 tataacgcta tatctataaa atatttatgt attttcctgt ccgtttcttt cgtgattttt
     7141 cctgatggta aactatcata aattcttttc attttctgaa tcaagcctat cactttttca
     7201 tgatcatcta ttttgaaaag agagagggct ttattgtcaa ttaacgtgtt tttatgatgt
     7261 ttgctgtaat tatctgattt tgttttcttt gtatgtgatg aacttgtgat agagtggtta
     7321 tcgttaatct ggcaaagagg gaaaaacata ttatttaaac cattatagcg cataaaatat
     7381 ccttataatt aacaagaaag aaaaggcata ttctctgcat tatcattttc tacgactgtc
     7441 aaaaatcgct cattttttaa tgagtttatt tgtttaatat tatgggaaaa ggtgatgcat
     7501 gttggagagg aagagtattc cccggtcagg cgaccgggga aggggagaga ttaatgtgcg
     7561 cggccttgct caacgccaaa gccggtctgg gagcggataa actgcgcgcg gaacagttca
     7621 cgctcgcgcg ccccttccgc tgagttatcg gttgccgaga agaaccagat gccgaggaat
     7681 gccacgctga tagagaacag cgccgggtat tcatacggga agatggcttt ttcgtgaccg
     7741 aggatctgta cccaaatagt cgggccgagg atcatcagca ccactgcggt aatcagcccc
     7801 agccagccgc ccagcatcgc gccacgggta gtcagtttcg accagtacat ggaaagcaga
     7861 atgatcggga agttacagct cgccgcgatg gcaaacgcaa ggcctaccat aaaggcgatg
     7921 ttctgattct caaacagcac gccgaggata atcgcaatca cgccgaggat cagtacggtg
     7981 atttttgata cccgcagctc ttcacgttcg gtcgcgcctt ttttgaagac gttagcgtac
     8041 aagtcatgcg aaaccgccga tgcgcccgcc agcgtcagac ccgcaaccac cgcgaggata
     8101 gtggcgaaag caaccgctga aataaaaccg aggaataggt tgccgcccac tgcattcgcc
     8161 aggtgaacgg ccgccatgtt gttaccacca atcagatggc ccgccgcgtc tttatattcc
     8221 ggattcgcac caacaagcat gatcgcgccg aagctgataa taaaggtcag aatatagaag
     8281 tagcccataa atccggtggc gtagaacacg ctcttacgtg cttcgcgggc atcgctgact
     8341 gtaaagaagc gcatcagaat gtgcggcaag cccgccgtac caaacatcag tcccagcccc
     8401 agagagagcg cggagatcgg atctttcacc agtccgcccg gtttcatgat gtcgacacct
     8461 ttcgggtgta ccgccatcgc ttcgctgaac agattgttga agctaaagcc gacgtgtttc
     8521 atcaccataa aggccataaa gctggcaccg aacagcaaca gcacagcttt gataatttgt
     8581 acccaggtgg tcgccagcat gccgccgaac aggacgtaca tcatcatcag cacgccgacc
     8641 agcatcaccg caatgtgata gttaaggcca aacagcagct cgatcagttt acctgcgcct
     8701 accatctgag cgataaggta aagcgccacc accaccagag aaccacaggc cgaaagaata
     8761 cgaatcggcc cttgtttcag acgataagag gccacatcgg cagaggtgta gcgtcccagg
     8821 ttacgcagac gttcggtaat cagaaacaga atgattggcc agcccaccag gaagcccaac
     8881 gagtagatca gcccgtcata gccggaggta aacaccagcg cggagatccc caagaatgag
     8941 gcggcggaca tatagtcccc ggcaatcgcc agcccgttct ggaagccagt gatattgccg
     9001 cctgcggtgt agtagtcgct acgagaacgt acgcgttttg atgcccagta ggtaatgccg
     9061 agcgtaaaca cgacgaaaat caggaacata ataatcgcct gccagttcgt tggctggcgc
     9121 tctacggccc cgctaatagc atccgcggcg ttagctgcga aagggagtgt ggcggcaagc
     9181 gccgtcagaa ctcttttcat gatgcttgta cctcatgcag gacttcatta ttaagacggt
     9241 cgaattcgcc gttcgcccgc cagatgtaga taccggtgag aacaaaggag atcacaatca
     9301 ctccaacacc aattggaata ccgcgtgtga cgctggtgtt cggattcagc ggcgtgccca
     9361 gccagccggg cgcgaaggcg atcagtaaaa taaagccgat ataaactgcc agcataataa
     9421 tcgacaggat ggtggcaaac cgttgccgtt tttcgactaa ctccctgaaa tgcgcattgt
     9481 cttctatccg ctgataaatc gtaccattca tcacagattc tccagaggta atgtagggat
     9541 tgttttaatt cccgctccct tatgggagaa ggttaacgct cgggtaaccc ttgccgaatg
     9601 taggccggat aaggcgttta cgccgcatcc ggcaatcaat gcctgatgcg acgctgtcgc
     9661 gtcttatcag gcctacaaac cgttaccgat tcgcatccgg caattgtggg ttacgatggc
     9721 atcgcgatag cctgcttctc ttcaagcagc ttctcgacca cgccaggatc ggcaagcgtc
     9781 gaggtatcgc ccaggttgct ggtatcgccc gccgcaattt tgcgcagaat acggcgcata
     9841 attttgccgg agcgggtttt aggcagggag tcggtccagt gcagcacgtc tggcgtcgcc
     9901 agcgggccaa tctctttacg cacccagttg cggacttctg cgtacagttc tggtgacggt
     9961 tcctccccgt gattaagcgt gacataggcg tagatcgcct gacctttaat attgtgcgga
    10021 atacctacta cggcggcttc ggcaatcttc ggatgcgcca ccagcgccga ctctatctct
    10081 gccgtcccca gacggtgacc ggagacgttc agcacgtcgt ccacacgccc ggtgatccag
    10141 taatagccat cttcatcgcg acgcgcgccg tcgccgctga aatacatatt tttgaaggtg
    10201 gaaaaatagg tctgctcaaa acgttcgtga tcgccaaaca gcgtacgcgc ctgaccaggc
    10261 caggagtcgg tgatcaccag gctaccttcg gtagcccctt ccagcgggtt accttcgtta
    10321 tcgaccagcg ccggttgcac gccgaagaac ggacgtgttg ccgaaccggc tttcagctcg
    10381 gtagcgccag gcagcggggt gatcatgaaa ccgccggttt cggtctgcca ccaggtatcg
    10441 accaccggac atttctcgtt gccgattttt ttccagtacc actcccacgc ttccgggtta
    10501 attggctcgc ccacggaacc gagaatgcgt agcgacgaac ggtcggtgcc ttcaatcgct
    10561 ttatcgcctt ccgccatcag cgcgcggatc gccgtgggtg cggtatagag aatattgacc
    10621 tgatgcttgt ccaccacctg cgccatacgg gcaggcgtcg gccagttcgg tacgccttca
    10681 aacatcagcg tggtcgcgcc gcaggccagc gggccgtaca gcaaataact gtgtccggta
    10741 acccagccca catcggcggt gcaccagtag atatcacccg gatgataatc aaagacatat
    10801 ttaaaggtca gcgccgcgta caccagataa ccgccggtag tgtgcagtac gccttttggt
    10861 tttccggtag aaccggaggt atagagaata aacagcggat cttcggcgtt catctcttcc
    10921 gcctggtgct gatcgctggc ttgctcaacc aggtcgtgcc accacaggtc gcgcccttcc
    10981 tgccagtcga tgtttccgcc agtacgcttc agtaccacca catgctctac gctggtgacg
    11041 ttcgggtttt ttagtgcgtc atcaacgttt ttcttcagcg gaatactacg cccggcgcgc
    11101 acgccttcgt cggaagtgat caccagtcgt gagttggaat caataatgcg cccggcaacg
    11161 gctttcggcg agaagccgcc gaaaatcacc gaatgcaccg cgccaatgcg ggcgcaggcc
    11221 agcatcgcaa ccgcggcttc cggcaccatc ggcatataaa tcgccaccac atcacctttt
    11281 ttaatgccca gctcgagcag ggtattggcg aagcggcaga cgtcgcggtg cagctcttta
    11341 tagctgatat gtttgctctg gctggcgtcg tcgccttccc agatgatggc ggtgcgatcg
    11401 ccgttttctt gcagatggcg gtcaaggcag tttgccgcca gattcagcgt gccgtcctcg
    11461 taccatttaa tggacacatt accgggggca aaggaggtat ttttcacctt ctggtacggt
    11521 ttgatccagt cgagaatttt tccctgttcg ccccagaagg tatcaggtac gttaatagat
    11581 tgttgataca tcgcctcgta ctgctgaggg tttatcaggc aacggtctgt gatgttggca
    11641 ggaatggtgt gtttgtgaat ttggctcatg cttttgttct ccttgtagga tgttaataat
    11701 atgtggcata agcgttaaat gtaggggtat tggcagtttt gtttagtatt tgggcgacag
    11761 atcacgcaaa agtagaatgg tgcaaataaa cggcagggta atttttgaag gtcaccaaca
    11821 aaagttgatt aattctttga ggaacatgca gttatgcatg ctgttgaaaa agaggaagat
    11881 actaactaac tctaaagtgg tattttacat gcacttacaa ttgattaaag acaacattca
    11941 cagtgtggtt atttgttaca cataggggtg agcaatgtca tgacagtgta ggtgcggtta
    12001 ctgtcgtgaa aaacaataaa aaccgccatt gcaacaatgg cgcaattcgg atgaagcccc
    12061 tatgacaagg ataaaaataa acgcacgccg tatcttcagc ttattgattc cttttttctt
    12121 tttcacttct gttcacgctg aacaaacggc agctcccgca aaacctgtaa ctgtggaagc
    12181 gaagaatgaa acctttgccc cgcagcatca cgatcaatat ctctcctgga aagccacctc
    12241 ggaacagtca gagcgtgttg acgccctgga ggaagatcca cggctggtga tcctgtgggc
    12301 ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga catgcctttg ctgtgaccga
    12361 tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct gaagatggtc cgctaccgat
    12421 ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg atccagaaag acggcgaaga
    12481 tggctacttc cacggtaaat gggcgcgcgg cggcccggaa atcgtcaaca acttaggttg
    12541 tgccgactgc cataacaccg cctcaccaga gttcgccaaa ggcaaaccgg agttaaccct
    12601 ttcccgtccg tatgcggctc gcgcgatgga agccattggt aaaccttttg agaaagccgg
    12661 acgtttcgac cagcaatcga tggtttgcgg tcagtgccat gtggagtatt acttcgacgg
    12721 caaaaacaaa gcggttaaat tcccgtggga tgacggcatg aaagtcgaaa atatggagca
    12781 gtattacgac aaaattgcct tctctgactg gactaactcc ctgtcgaaaa cgccaatgct
    12841 gaaagcgcag cacccggaat atgaaacctg gacagcgggc attcacggta aaaacaacgt
    12901 gacctgtatc gactgccata tgccaaaagt gcagaacgcc gaaggcaaac tctacaccga
    12961 ccataaaatt ggtaatccgt ttgataactt cgcccagact tgtgcgaact gccataccca
    13021 ggataaagct gccttgcaaa aagtggtcgc ggaacgtaag caatcgatta acgacctgaa
    13081 aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa gcgaaagcgg tgctggatgc
    13141 aggcgcgacg gaagccgaaa tgaagccgat tcaggacgat atccgtcatg cccagtggcg
    13201 ttgggatctg gcgatcgctt cccacggtat tcatatgcac gcaccggaag aaggtctacg
    13261 gatgctcggt acggcgatgg ataaagcggc ggatgcgcgc accaaactgg cgcgcctgct
    13321 ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat atctcgacca aagagaaagc
    13381 ccagcaggcc attggcctga acatggaaca aatcaaggcc gagaagcagg acttcatcaa
    13441 aacggtgatc ccgcagtggg aagagcaggc acgtaaaaac ggtctgttaa gccaataacc
    13501 ccgttccgcc tcgcaagggg cggaaaacac aatggagtga atatgagcgt attacgttcg
    13561 ttattaactg ctggggtgct ggcgtcgggc ctgttgtgga gcctgaacgg aattaccgct
    13621 acccctgcgg tgcaggcatc tggtgatcgc tacgaagtta cccagcagcg taacccggat
    13681 gccgcctgtc tggactgtca taaaccagat accgaaggta tgcatggcaa acatgcttcc
    13741 gttatcaacc cgaataacaa attgccggtc acctgcacca actgtcacgg ccagccatca
    13801 ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta acgagccgat gtacaaggtc
    13861 ggagagcaga acagcgtctg tatgtcctgt catctgccgg aacagttgca aaaagcgttc
    13921 tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca gctgccattc cctgcatcca
    13981 cagcaagata cgatgcagac gttaagtgac aaaggacgga ttaagatttg cgtcgattgc
    14041 cacagcgatc agcgcaccaa tccgaacttt aacccggcgt ctgttccgtt gcttaaggag
    14101 cagccatgac ctggtctcgt cgccagtttc tcaccggcgt cggcgtgctg gcagccgtca
    14161 gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat caatggggtg cgttacggca
    14221 tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg tatggatgct tgtcgggaag
    14281 tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat tcgtagcgag ccgcagggcg
    14341 aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg ccagcactgc gagcatgcgc
    14401 cgtgcgttga cgtctgcccg accggtgcgt cttttcgtga tgccgctagc gggattgtcg
    14461 atgttaatcc tgatctctgc gtcggttgtc agtactgcat cgccgcctgt ccgtaccgcg
    14521 tgcgctttat ccatccggtc acgaagacgg cggacaaatg cgatttctgt cgtaagacca
    14581 atttgcaggc cggtaagctt cccgcgtgcg ttgaagcttg cccgacgaag gcgctgacgt
    14641 ttggcaatct ggacgatcct aacagtgaga tttcgcaact gctgcgccag aagcctactt
    14701 accgctacaa gctggcgctg ggaaccaaac cgaagctgta ccgcgtaccg tttaaatacg
    14761 gggaggtgag ccaatgacgc agacttccgc atttcatttt gaatcgctgg tgtgggactg
    14821 gccgattgcc atctacctgt ttttgattgg tatttctgcc ggtctggtga cgctggccgt
    14881 gctgttacgt cgcttctacc cgcaggcggg cggtgcagac agtacgttgc tgcgcaccac
    14941 gctgattgtc gggccgggcg cggtgatcct cggtctgttg atcctcgtct tccacctgac
    15001 aagaccgtgg accttctgga agctgatgtt ccactacagt tttacctcgg tgatgtcgat
    15061 gggggtgatg ctgtttcagc tctacatggt ggtgctggtg ctgtggctgg cgaaaatctt
    15121 tgaatatgat ttgcttgccc tgcaacaacg ctggttgccg aagctgggga tcgtgcaaaa
    15181 ggttctgagc cttctgacgc ccgttcatcg cggactggaa acgctgatgc tggtgttggc
    15241 ggtgctgctg ggggcttata ccggctttct gctgtcggcg ctgaaatcgt atccgttcct
    15301 caataacccg agcctgccgg tgctgctcct cttctccggc atctcgtccg gtgcggcggt
    15361 ggcgctgatc gccatggcga tacgccaacg cagtaacccg cattccacgg aagcgcactt
    15421 tgttcaccgg atggaaatcc ccgtggtatg gggtgaaatc ttcctgctgg tggcgttttt
    15481 tgtcggtctg gcgctgggcg atgacggtaa agttcgtgcg ctggtggcgg cattaggtgg
    15541 cggtttctgg acgtggtggt tctggcttgg tgtcgccggg ctggggctga ttgtgccaat
    15601 gttgctcaaa ccgtgggtca atcgcagttc cggcattcct gccgtgctgg cggtgtgtgg
    15661 ggccagtctg gtcggcgtgt tgatgctgcg ctttttcatt ctctacgccg gacagttgac
    15721 ggtggcgtaa gccagaaaag aggtggtttc tggacgtatt ccttcctgaa gtcggttttc
    15781 tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc gttgacggcc tttgcgggag
    15841 tgcggttgcg ctggcctgcc atgatgcgac tcacttgcat cggcattctg gcgcagttcg
    15901 cgctcctgct gctcgccttt ggcgtactga cgtattgttt tctcatcagc gatttctcgg
    15961 tcatttatgt cgcccaacat agctacagcc tgctgtcgtg ggaacttaaa ctggcggcgg
    16021 tgtggggcgg tcatgaaggt tcgctgctgc tttgggtgct gctgctttcc gcctggagcg
    16081 cgctgtttgc ctggcattat cggcagcaaa gcgatccgct atttccgctg acgctagccg
    16141 ttttatctct catgctcgcc gcactgctac tgtttgtggt gctgtggtcc gatcccttcg
    16201 tgcggatatt tccaccagca atcgaaggcc gcgatctcaa tccgatgctg caacatcccg
    16261 gtcttatctt tcatccaccg ctgctttacc ttggctatgg cggtttgatg gtagcggcga
    16321 gcgtggcgct ggcgagtcta ctgcgcggcg agtttgatgc ggccagcgcc cgaatttgct
    16381 ggcgctgggc gttacctggc tggagtgcat taacggcggg gatcatcctc ggttcctggt
    16441 gggcctactg cgaactcggc tggggcggct ggtggttctg ggatccggtg gaaaacgcct
    16501 ctttattacc ctggctttct gccactgcgc tgctgcacag tttgtccctg acacgccagc
    16561 gggggatttt ccgccactgg tcgctgttgc tggcgatagt tactctgatg ctgtcgctgc
    16621 tgggcacctt aattgtccgt tctggcattc tggtttcggt tcatgcgttc gcactggata
    16681 acgtccgcgc cgtgccgttg ttcagcctgt ttgcactgat tagccttgcg tctttggctc
    16741 tgtatggctg gcgagcgcgg gacggtggcc cggcggtgcg tttttcgggg ttatcgcggg
    16801 aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt gctactgatc gtgctggtgg
    16861 gaacgcttta tccgatgatt tacggcctgc tgggctgggg acgcctctcc gttggcgcgc
    16921 cgtattttaa ccgcgcgacg ttaccgtttg gtctgttgat gctggtggtg attgtgctgg
    16981 cggcgtttgt ctctggcaaa cgcgtgcagc ttccggcgct ggtagctcat gctggcgtgc
    17041 tgttatttgc cgcggggatc gtggtttcca gtgtcagtcg tcaggagatc agcctgaatt
    17101 tacagccggg tcagcaggtg acgctggcag gatacacctt ccgttttgag cgactcgatc
    17161 tgcaagccag aggcaattac accagcgaaa aagcgatagt ggcgctgttt gaccatcagc
    17221 aacgcattgg tgaattaacg ccggagcggc gtttttatga agcacgccgt cagcaaatga
    17281 tggaaccgtc aattcgctgg aacggcatcc atgactggta tgcggtcatg ggtgaaaaaa
    17341 ccagagcgga tcgttacgct tttcgtttgt atgtacaaag cggtgtgcgc tggatctggg
    17401 ggggaggatt gttgatgatt gcgggcgcat tgttaagcgg atggcggggg aagaagcgcg
    17461 atgaataaag ggcttctcac gctactgctg ttatttacct gttttgcgcg cgctcaggtt
    17521 gtagacacct ggcaattcgc caatccgcaa caacagcaac aggcgttaaa tattgccagc
    17581 cagttacgtt gtccgcagtg ccagaatcaa aacttactcg aatccaatgc gccggtggcg
    17641 gtcagtatgc gccatcaggt ttatagcatg gtgtcggagg ggaagaacga agtcgaaatc
    17701 attggctgga tgaccgaacg ctacggagat tttgttcgct ataacccacc gttaacgggt
    17761 cagacgctgg tgttgtgggc gctgccagtg gtgttgttat tgctgatggc actgatcctc
    17821 tggcgagtga gggctaagcg atgaaacagc ctcaaatacc ggtgaaaatg ctgacaacac
    17881 tcacgatatt gatggtattc ctctgtgtcg gcagttatct gttaagccca aaatggcagg
    17941 cggtacgtgc ggagtatcag cgtcagcgcg atccgctaca tcagtttgcc agccagcaaa
    18001 ccccggaagc gcagcttcag gcattgcagg ataaaatccg cgctaatcca caaaacagcg
    18061 aacaatgggc gttactgggc gagtactatc tgtggcaaaa cgattacagc aattcgctgc
    18121 tggcgtatcg tcaggcgttg caactacgtg gtgagaacgc tgaactgtat gcggcgctgg
    18181 cgacggtgct ttattaccag gccagccagc atatgaccgc ccagactcgc gcaatgatcg
    18241 acaaagccct cgcgctggac tctaacgaaa tcaccgctct gatgctgctg gcttccgatg
    18301 cgtttatgca ggcgaattac gcgcaagcca tcgaactatg gcaaaaagtg atggatctca
    18361 actcaccccg gatcaaccgg acacagctgg tcgagtcgat taatatggca aaactgttgc
    18421 agcggagatc ggattaatca tctggtgatg aagattatgt cattgagctg cataaaaaat
    18481 aatcgaatga acatatgcca aaaataatca ccaatcagta ttattgcaga ttaaacaaat
    18541 aaaaatcttt ccataacaaa tggttattca ttaatcctgc cataaactcg cgttattatg
    18601 cattaatgca gcgaaaagct ctgttgttaa agggttgcgc aacataccgc gcaaatgata
    18661 ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac acaattcatg ccctggcagt
    18721 atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc attgaggaag ttattctgat
    18781 gaaaaatata aaattcagcc tggcctggca gattctgttt gctatggtgc tgggcattct
    18841 cctgggaagc tacctgcact accatagcga cagccgcgac tggctggtcg tcaatttgct
    18901 ctctccggcg ggtgatatct tcatccatct gatcaaaatg attgttgtgc cgattgtgat
    18961 ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc aaacagctcg ggcgtattgg
    19021 cgcgaaaacc attatttact tcgaggtgat caccaccgtc gccatcattt tggggatcac
    19081 tctggcgaac gtcttccagc ccggtgccgg ggtggatatg tcgcagctgg cgaccgtcga
    19141 tatctcgaaa tatcagagca ctacggaagc ggtacaaagc agttcccacg gcattatggg
    19201 cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg atggcgaaag gcgaaatgct
    19261 gccgatcatc tttttctcgg tgctgtttgg tctggggctt tcttccctgc ccgcgacgca
    19321 tcgtgaaccg ctggtgaccg tgttccgctc catctctgaa accatgttta aagtgactca
    19381 catggtgatg cgttatgcac cggtgggtgt gtttgcgctg attgcggtga cggtggctaa
    19441 ctttggtttc tcgtctctgt ggccactggc gaaactggtg ctgctggtgc atttcgccat
    19501 tctgttcttc gcgctggtag tgctgggaat tgtggcacgt ctgtgcgggt taagcgtctg
    19561 gagcctgatt cgtattctga aagatgagct gattctggcg tactccactg ccagctctga
    19621 aagcgtactg ccgcgaatta ttgagaagat ggaagcctac ggagcaccgg cgtcgatcac
    19681 cagcttcgtg gtgccgaccg gttactcttt taaccttgat ggttcgacgc tgtatcaaag
    19741 tattgccgct attttcatcg ctcagctgta tggcattgac ctgtccatct ggcaggaaat
    19801 cattctggtg ctgacgctga tggtgacctc gaaagggatt gctggcgtgg ctggcgtgtc
    19861 gtttgtggtg ttgctggcaa cgctgggtag cgtaggtatt ccgctggaag gtctggcgtt
    19921 tattgctggt gttgaccgta tcctcgacat ggcgcgtact gcgctgaacg tggtgggtaa
    19981 tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa tttgaccgca aaaaggcgct
    20041 ggcttatgag cgtgaagtgc tgggcaaatt tgataaaact gcggatcaat aattgaagat
    20101 tgccggggat atcccccggc aatgtgtgga tgcctgatgc gacgcttgcc gcgtcttatc
    20161 aggcctacct taaccgttgg ttaatttttc aaactcttca cacccggtat caaacccttc
    20221 catacagctc aggttcaacc agtgcagcgc cttttgtttg ttcttctcga taaaaccttc
    20281 ctcaccgttt aagaacatca ttcccgccca gtactcggaa taaccggttc gggaaattgc
    20341 agagctgcgt ttgaaatacc aggttgcctt gtcatcgtcg gccttaatgc ccacgccgtt
    20401 ggcgtaaatc aaaccaagca gcatttgggc atcgacggcg gagtcattct ccagatcttc
    20461 cgaggcgttt tctaacagcg aaatcgcctt tggataatcc ggtttacccg cttgagtatt
    20521 taccagaata tgcgccagcg ttacttcacc ttctttactg cccgcttgcg ccgctttctc
    20581 tgcaagaact tttgcttgtg gatagtccag actgaccgga ttggtgattt tgatctgcgc
    20641 caacagcgcg caggcatcag catcaccgct gtcggcggct ttctgtgccc aatattcggc
    20701 tttgctcaaa tcgccggagc taaaccagct gtcggcaaga aaatattgtg cgcgttggtc
    20761 cccggcctct gctgccttta aatactggct gccaggctcg gagtcgttcg cgtgggcaaa
    20821 gaatgtcaaa aacaacatca atgcgataat ttttttcatt ttgataatac gattgggtag
    20881 ggagtaacca gtataaagag ttcgaatgga taaaaaaaca gcctccgaaa ggaggctgta
    20941 gaaaggactg tattacgcca gtgccgcttc gcgcaggcga gttttcaact tgttgtactc
    21001 gtcaatcagg tactgctcgg cggcgcgctg atcggagatc ggctcgacgc gcacggcgca
    21061 gtatttgtat tccggcgttt tcgtaatcgg gcttaagttt tcggtaacca gctcgttaca
    21121 ggcaccaatc caccactggt aggtcatgta aatcgcccct ttgttcggac gatcgctgac
    21181 ctgcgcacgg gtgataattt tgcctttacg cgagtgcact caaaccaatg cctcatcttc
    21241 aatacccaga cgtttggcgt cttcggtatt gatttgtgcg tagccaggtt catcagccag
    21301 cgccgccagt gccgcacagt taccggtcat cgaacggcaa gagtagtgac caacttcacg
    21361 caccgttgac agtaccatcg ggtactcgtc ggtgagtttg tcgattggcg ctacccagtc
    21421 gcaggtgaag aactgcgcca gaccgttcgg ggtatcaaac ttctctttaa acagataaga
    21481 agtcccctga tcggcatctg aagtatcgcg gcaaggccac tgaatgaagc ccagttcgcc
    21541 cattttctcg taagtcgcac cgtagaaatc cgggcacaga tgacgcaact catcccagat
    21601 ctcctgggtg ttgttgtagt gcatcggata acccatacgg gtggcgattt cactgatgat
    21661 ttgccagtcc gttttcagat cccatttcgg ttcaactgct ttgaagaaac gctggaagcc
    21721 acggtcagcc gcagtaaaca cgccttcatg ctcgccccac gacgttgacg gcaaaatgac
    21781 atccgccgcc gacgcggttt tggtcataaa gatgtcctga acgatgacca gttccagatc
    21841 ttcaaaggct ttacgtactg ccgacagctc cgcatcagtt tgcagcggat cttcgcccat
    21901 aatgtacgcg gcacgcactt cgccatgcgc tgcgcggtgc ggcagctcgc tgatgcgata
    21961 gccggtatga gcaggcaggc tttccacgcc ccaggctttg gcgaatttct cgcggttagc
    22021 cggatctttc acgtactggt atcccggata cgtatccggc agcgcgccca tatcgcaggc
    22081 accctgcacg ttgttctgac cacgaaccgg gtttacaccc gcatgcggct taccgaggtt
    22141 accggtcagc atcgcgaggc tggtcagaga acgcacggtt tccacgccct ggtagaactg
    22201 ggttacaccc atgccccaca ggatggcggc gcttttcgcc tgggcataca tccgtgccgc
    22261 ctgacgaatc tcactggcgc tgacaccggt gatatcttca accgactccg gcgtgtagcc
    22321 ttcaacgatt ttacgatact cttcaaagcc ttctgtacgg gaagcgacga acgctttgtc
    22381 gtacagattt tcttcaataa tgacatggcc catcgcattc aacagcgcga tgttcgagcc
    22441 gtttttcagt gcaatgtgca tgtcagcaat gcgcgcggtt tcaattttgc gcggatcgca
    22501 gacgataatt ttcgccccgt tacgtttagc gttaattacg tgattcgcca cgattgggtg
    22561 ggaatccgcc gggttgtacc cgaaaacgaa cactaaatcg gtattatcaa tttcgttaat
    22621 agcattgctc attgcgccat taccgaccga ttggtgcaga cctgcaaccg atgggccgtg
    22681 tcagacacga gcgcagcagt caacgttatt ggtaccaata acggcgcgcg caaatttttg
    22741 cattacatag ttggtttcgt tacccgtacc acgcgaggag ccggtcgtct ggatggcatc
    22801 cggaccgtac ttctctttga tggcgctcag gcgctcggca acgtaattca gtgcctcatc
    22861 tcaggaaaca ggttcgagtt tgccgccacg ctggcgacgg atcatggggg ttttcaggcg
    22921 cggggtcagg atctgggtat cgttaatgaa atcccagcca taataaccct tcagacacag
    22981 ggtaccctgg ttggttttcc cctgcgctgc ctccgcccgg acgattttgc cgttatcgac
    23041 gaccaggttg attttgcaac ctgatgcgca ataggggcaa accgtgacga cttttttcat
    23101 cggtctcgct ccagttaatc aaatcacgca tacgcgctct cgactacagt atgcatcttt
    23161 tatgccacat tttatttggg gtcattccct gatattacgg gcactattta ttcaaaactc
    23221 tgacgaaaaa caggctgtcg tcagttttga cgtgacgaaa cgaaataccg cgtgacagcc
    23281 atcacgcggc agacatttta ttttttctcg acgacgggag ccgcctgata cccgccgccc
    23341 agcgatttca tcaactgaat gctttggatc acccggcggc tgtccagcat cagtaatgac
    23401 atctcttcgg caagtactgg caaccgtgct tcagtggcct gtaagcggct ggttaagccg
    23461 cgctgatagg cggcctcggc agcgcgctgg gtgaagcgcg ttgcttccac gtgttcagcc
    23521 tgcatttctc gctcgtcgtt gagcgtttgc agacgcgtgc cgttgacggc aacgtcacgc
    23581 accgcgttca gtactgactg gttgtaacgt tcaatcatca tgttgctggc ggcgcgcgtg
    23641 ccttcgagat tggcattcaa ccgtccaccg tcaaacagcg gcaatttcag acccgggatg
    23701 aagttgaact ggcgactggt ttttttgaat aaggtatcta ggtggatggc atcaaggccg
    23761 aaaaacgctt tgatatcaaa gctcggatag aacaacgccc gcgcggaatc cacctgatct
    23821 aatgacgcct gaacatacca gcgcatggct tgcagatccg ggcgtctggc gagcaactca
    23881 taagagagtg tcgccggaat gccggtctgg actcgtggta atgccacagg tttgatctcc
    23941 ggcatatcgc tggctcccgc gccaatcaat gcgcgcagag attctctcgt ttcggtgatt
    24001 tgccctttga cggcagcaat ttgtttatcg accgccagaa tctgtgcccg cgcgccgtgg
    24061 aaaggcactt gtgcttccag accgtgcgcc actttacttt ggtgcgcttt caccgcgtaa
    24121 tcaatcacat cgcgagtttg ttctaacaga tcgagcatct gatagctggc ctgcatactg
    24181 taataaagct gcgctacgcc cgtggtcagc gatagctcta ctgctgcggt ttctgccagc
    24241 gcggcattat gcgcgccaat ggcggcggca accgctgagc gatgcacacc ccacaaatca
    24301 agatccagtc cggcaaacaa acctactgtg gcttccgtat agtacggccc gtccatcccc
    24361 agtgcgggcg catccatcgc ataagggctt aaaaagccgt tcgccgagac acgttggcgg
    24421 ttaagcatcc ctaacgctgc cacctgcaac tgtgaaccgg catctaacaa atcggcctgc
    24481 gattgcgctt tttcttcccg cagtttcgct tcggcgaggg tgtgtgaacc acttagcgtc
    24541 cgttggatca gggcatccaa ctgcgggtca ttgagttgtt tccaccactg cgcctgcggc
    24601 cagccggagc tggcaagatg aatatcatcg gccagtttga tttgttccgg tttgagctgt
    24661 tgatgaggcg cagaatcctt acgtaccagg gcacagccgg aaatcagcgt cgtgctgccg
    24721 agaatgctgc acaacagcag acgtgaaagt tgacgattga tcattgcgtg gctccttgtg
    24781 cctgttccgt ggcgggcgtt aatgccgggg ctgtccagtc tggcaagcgg gcgaaaagct
    24841 ggcagacgtg ttgctcctgt tttaataagg tgggcaggat ggcctgcgag gcgggtgtct
    24901 cttcaagcgt tatttgagga gaacggctga gtgcggttgc cagaccggca gcgtatttct
    24961 ccagggcgtc ggcaaactga cctgctctgt ccggctgtag tgcgttatcc agcgcctgcg
    25021 ccgaattcca ggtggcatcc caggcgtgaa ggatatcgcg gccctgacga ataaccgttt
    25081 gcgaacgttc aatcagtaag gcgcgttctt cgctgtccag ttgacgctcc agcgccacgc
    25141 gttggcacat ctcttcacag gcattaaacg ccgcatgcag accgatacga atttgcagat
    25201 aagtgcgcag agccgtgact tcctgctggc gtgggatccg cattacttta cttaacatac
    25261 ccagcgcgcc agccagtttt tgcggcagcg tgcgcgcttc actttcaggc cagacaaagg
    25321 tgtaaatcat cgcggacacc acggtaccaa tgatgatacc cagggcgcga tcgcgaattt
    25381 ccaccaggtc gtacactggg ccaaaaacgt tttcgagcgt ggcgagcgcg aaggtgacca
    25441 ccatctgtgt gccgatataa gaagagcgtt cagagctggt ggcaatctat gcgcccaaca
    25501 ggaaaatcgg tgccagcaca aacagcaatt cgacaatatt gtccagccag ggcatgacca
    25561 gtagcgtgaa taacagcgcc agaatcgcgc cgcaaaaggc cccgccaaaa cgcagcacca
    25621 tcttctggta cgacgaaccg acatttggat tagcgacgat cacgcaggtc agcatacagg
    25681 tgtgaatgcc ttcccagtcc acgccgctgt aaaaggtgta acagatcaaa caggcgagca
    25741 atgtttttac cgcgtagcgt atatagtctg gattggtaaa agcatcggcg accattgatg
    25801 gcggtttggc ggcgggcgtt ggcggcgtat tcgggttcat ctggcccagt tgtaacaacg
    25861 tctggcagat attctccagg ttacattccc gtgccgccac cgcttcactt tcactgatcc
    25921 gccagtcgct ttgccagcac tgaccttcag caacggcatg ctgcagcttg ttgatttctg
    25981 aggctaattt ttgccgaaat tcaataattg cctgagaatc agcaaaagag gtgggatcgt
    26041 agcgattcag cgtcgagtaa atgtaggtta ccgttgccac gcagctttgc caccatgcgc
    26101 tttgagttcg ccagttggca tcgtccgcga gacaaaagac attcagtttt tgcagcgcca
    26161 gcgcctctct ttcaatccgc gtttcgggta gcggtgcgag gctgtccgtc aggtggctaa
    26221 tggcatcatc aagccgatca ttaagcgctt gatgcatttg cgtaatggca cgattgggaa
    26281 accacagcac gccgattaac gtcatcagca aggttggata gaggccaaca acgatacacc
    26341 acagcgttaa gcgcacgact acttccggat agtcgagcat ggcggggaag gtttgcccgt
    26401 aaatggcgac aatggcgaca gcgaaaaaga ccagtcccag ccgatgggtg cgcatcaaaa
    26461 acatgcagcc catcaggatc ggcccggcga tgatcaatcg gatcaacggt tcgccgtatg
    26521 accatttgta gatcaaaaac aggctgccga tctccagcac cgtggcaacc acaaacaaga
    26581 tcgccacaaa tttggtgtaa aacgcgttcg actgaatacc gtaaaacagc actgccagcg
    26641 ataacgccac aaaagggatc tcaaaagtca tcgagatcag aatcaccagc aggcagccta
    26701 cccagagttg cacgatctgc ggcacgcggc cgggtcgccg ctcgcttaac tcttcatgaa
    26761 agaacgccag cagcctgacc accggtaatg gcagggagtt gagcgcgctc attattgcgg
    26821 ctcaagatta gcgactgccg aagcgccgat gcggaacatt tccgggtcag gtttatcgac
    26881 catgattttg accggaaaac gctgggcaac gcggacccag ttaatagaac gagacacttt
    26941 tggcaggccg cccagcacca ggccgccgtc atccggtagc acgccgtagc caatcgaatc
    27001 cactttaccc tcgaaggttt tgccgctgtc actcatcagg cgaatcgttg cgggtgtacc
    27061 tgagcgaata tttttcagat cggtttcgcg gaagttggcg atcacgtacc agtgacgagt
    27121 gtcgatgagg gtaaaaatag ggcgcatggc agaagcaaat tgcccgacgg aggttttgag
    27181 ggaaatgatc cggccatcaa atggcgcgcg aacggtcgtc atttccagat gtagtttggt
    27241 cagggcaata tccgcttcga gcgccgcacg ttgcgccacc agcgcatcga cgccgctgac
    27301 ggcgctggcg gctgactgcg cctgtaacaa tacggcatta agatccgctt ctgcggcgcg
    27361 ctgcgccgtt cttgcacggt caacgtcttc tgctgagaca aaaccctctt tcagtaatgg
    27421 ctcggtgcgg cgtaatgtat ctgtggcctg tttcgcggcg gcacgggctt tttctaccgt
    27481 ggcattaacc gagtcgtcac caaactgttg ggcgtcaacg ctacgctggg tgagcataat
    27541 ttgcttatcc agcgccgcga gggaggcttc ggctttcgcc aggttggctt cgtacgggcg
    27601 cgggtcgatg cggaacagca aatcgccctg tttcaccgcc tggttgtcgg tgaccgccag
    27661 ttctacaatg cgaccgctga cttccggcac cacatcaatg gtatctgctg acgcgtaagc
    27721 gtcattagtt gatggcgcac tgtctacgcg ccagataacg aaaacaaggg caaccagcgc
    27781 caacgcaacc actaacagag cagggaattt actgcgaggt gcttttttcg gcgtactttc
    27841 catgagcgcg tctcaaatag attagaaaaa tgccagccag aacagcatgg cgtagagagc
    27901 aaaaagggcg gtataaataa ttccgacaaa tgccagattg atatttgccc gctgaataac
    27961 tcgcctcgtg atgagcgtca aaataaggct ggcaatggcg cagaaaaacc agctgggata
    28021 ataagcgccg atcaccggga ttgccggaga taatgagcat cccgaaacca tgaagacggg
    28081 aattatccag gctgtttttt tgaggtgtat cgccatgcga gagagaactg ttggcatatt
    28141 aaatacatcc tgtctgctgc gaacaatatc tgtagtgaat atcaatatcc attgaaggcg
    28201 tcagtatggc tattttctcg atgaataaaa atagctttat tcagccaccg cattcataac
    28261 ttatatattt tatttattaa ttggctgtat atatttttaa aataaatgct tttgggggct
    28321 tcagcgcccc caaaagcgag tgacattaat ttggggttac gatattcacc cacaaatcga
    28381 aattatccag acaggcgata atttcttcca gtttcgcgcc attgccaata attttggctc
    28441 tcttcgcttt caccagatcc gcc
//
LOCUS       DAETPZ010000047        28257 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.047, whole genome shotgun sequence.
ACCESSION   DAETPZ010000047 DAETPZ010000000
VERSION     DAETPZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28257
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.047"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..145
                     /locus_tag="K0W59_002325"
     CDS             <1..145
                     /locus_tag="K0W59_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3023333.1"
                     /translation="YTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIK
                     HYQ"
     gene            complement(157..357)
                     /locus_tag="K0W59_002326"
                     /pseudo
     CDS             complement(157..357)
                     /locus_tag="K0W59_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            551..1714
                     /gene="yqhD"
                     /locus_tag="K0W59_002327"
     CDS             551..1714
                     /gene="yqhD"
                     /locus_tag="K0W59_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBI3023334.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYKVAR"
     gene            1819..2646
                     /gene="dkgA"
                     /locus_tag="K0W59_002328"
     CDS             1819..2646
                     /gene="dkgA"
                     /locus_tag="K0W59_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HBI3023335.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKCPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDEPGKIAKLDQGKRLGPDPDQFGG"
     gene            2846..3772
                     /locus_tag="K0W59_002329"
     CDS             2846..3772
                     /locus_tag="K0W59_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HBI3023336.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKLIEAKTAARLKQ"
     gene            3823..4080
                     /gene="yqhH"
                     /locus_tag="K0W59_002330"
     CDS             3823..4080
                     /gene="yqhH"
                     /locus_tag="K0W59_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HBI3023337.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(4123..6342)
                     /locus_tag="K0W59_002331"
     CDS             complement(4123..6342)
                     /locus_tag="K0W59_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBI3023338.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDCVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSLINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(6453..7865)
                     /gene="ftsP"
                     /locus_tag="K0W59_002332"
     CDS             complement(6453..7865)
                     /gene="ftsP"
                     /locus_tag="K0W59_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HBI3023339.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(7940..8677)
                     /gene="plsC"
                     /locus_tag="K0W59_002333"
                     /gene_synonym="parF"
     CDS             complement(7940..8677)
                     /gene="plsC"
                     /locus_tag="K0W59_002333"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HBI3023340.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(8911..11169)
                     /gene="parC"
                     /locus_tag="K0W59_002334"
     CDS             complement(8911..11169)
                     /gene="parC"
                     /locus_tag="K0W59_002334"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBI3023341.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNARAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(11715..12197)
                     /locus_tag="K0W59_002335"
     CDS             complement(11715..12197)
                     /locus_tag="K0W59_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI3023342.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(12250..12642)
                     /gene="ygiW"
                     /locus_tag="K0W59_002336"
     CDS             complement(12250..12642)
                     /gene="ygiW"
                     /locus_tag="K0W59_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HBI3023343.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESVKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            12794..13453
                     /gene="qseB"
                     /locus_tag="K0W59_002337"
     CDS             12794..13453
                     /gene="qseB"
                     /locus_tag="K0W59_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HBI3023344.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLSSDFIRTVHGIGYTLGE
                     K"
     gene            13450..14799
                     /gene="qseC"
                     /locus_tag="K0W59_002338"
     CDS             13450..14799
                     /gene="qseC"
                     /locus_tag="K0W59_002338"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HBI3023345.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTPDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLIGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLLALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(14845..15177)
                     /locus_tag="K0W59_002339"
     CDS             complement(14845..15177)
                     /locus_tag="K0W59_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HBI3023346.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            15496..16077
                     /gene="mdaB"
                     /locus_tag="K0W59_002340"
     CDS             15496..16077
                     /gene="mdaB"
                     /locus_tag="K0W59_002340"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HBI3023347.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            16108..16422
                     /locus_tag="K0W59_002341"
     CDS             16108..16422
                     /locus_tag="K0W59_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBI3023348.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSKAVKGDVLEMNIRILQP
                     GI"
     gene            complement(16470..18362)
                     /gene="parE"
                     /locus_tag="K0W59_002342"
     CDS             complement(16470..18362)
                     /gene="parE"
                     /locus_tag="K0W59_002342"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBI3023349.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     NGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(18391..18972)
                     /gene="yqiA"
                     /locus_tag="K0W59_002343"
     CDS             complement(18391..18972)
                     /gene="yqiA"
                     /locus_tag="K0W59_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HBI3023350.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(18972..19799)
                     /gene="cpdA"
                     /locus_tag="K0W59_002344"
     CDS             complement(18972..19799)
                     /gene="cpdA"
                     /locus_tag="K0W59_002344"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HBI3023351.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(19824..20246)
                     /locus_tag="K0W59_002345"
     CDS             complement(19824..20246)
                     /locus_tag="K0W59_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HBI3023352.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(20247..20876)
                     /gene="nudF"
                     /locus_tag="K0W59_002346"
     CDS             complement(20247..20876)
                     /gene="nudF"
                     /locus_tag="K0W59_002346"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HBI3023353.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            21081..22562
                     /gene="tolC"
                     /locus_tag="K0W59_002347"
     CDS             21081..22562
                     /gene="tolC"
                     /locus_tag="K0W59_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HBI3023354.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            22710..23381
                     /locus_tag="K0W59_002348"
     CDS             22710..23381
                     /locus_tag="K0W59_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HBI3023355.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            23387..24547
                     /locus_tag="K0W59_002349"
     CDS             23387..24547
                     /locus_tag="K0W59_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBI3023356.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQCGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(24585..25373)
                     /gene="ygiD"
                     /locus_tag="K0W59_002350"
     CDS             complement(24585..25373)
                     /gene="ygiD"
                     /locus_tag="K0W59_002350"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HBI3023357.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            25516..26289
                     /gene="zupT"
                     /locus_tag="K0W59_002351"
     CDS             25516..26289
                     /gene="zupT"
                     /locus_tag="K0W59_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBI3023358.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(26347..26517)
                     /locus_tag="K0W59_002352"
     CDS             complement(26347..26517)
                     /locus_tag="K0W59_002352"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023359.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(26778..27431)
                     /gene="ribB"
                     /locus_tag="K0W59_002353"
     CDS             complement(26778..27431)
                     /gene="ribB"
                     /locus_tag="K0W59_002353"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HBI3023360.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(27536..27684)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            27806..28096
                     /gene="ubiK"
                     /locus_tag="K0W59_002354"
     CDS             27806..28096
                     /gene="ubiK"
                     /locus_tag="K0W59_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HBI3023361.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT         7144 a         7490 c         6993 g         6630 t
ORIGIN      
        1 ttacactcag cgcattgagc gacataatct gaatctgaga caacatctgg caaggctggg
       61 acggaagtca ctgtcgttct caaaatcggt ggagctgcat gacaaggtca tcgggcatta
      121 tctgaacata aaacactatc agtaagttgg agtcattacc cgttcattga tataaccgat
      181 tttatgcccg gaaaagagaa ttatgatgcc aggctcgtac atcaccggtg tacgtgcgaa
      241 aggcgtctcg ccatacaaca aacgcacatc gggcaacagt tctgacaaac tattttcttt
      301 atttttcagt ttattaactt tatccgccag caagcgacaa atctcttcac gtttcatatc
      361 gcgtaatttc ttaggaataa tgcggcaatt tgattgtgcg caattttgta gcatttctcc
      421 agcactctgg agaaataggc aagacattgg cagaaatgag cattgagagc cagggcgctg
      481 gcgatcacaa tgaaaaacat caggcagatc gttctctgcc ctcatattgg cccagcaaag
      541 ggagcaagta atgaacaact ttaatctgca caccccaacc cgcattctgt ttggtaaagg
      601 cgcaatcgct ggtttacgcg aacaaattcc tcacgatgct cgcgtattga ttacctacgg
      661 tggcggcagc gtgaaaaaaa ccggcgttct cgatcaagtt ctggatgccc tgaaaggcat
      721 ggacgtgctg gaatttggcg gtattgagcc aaacccggct tatgaaacgc tgatgaacgc
      781 cgtgaaactg gttcgcgaac agaaagtgac tttcctgctg gcggttggcg gcggttctgt
      841 actggacggc accaaattta tcgccgcagc ggctaactat ccggaaaata tcgatccgtg
      901 gcacattctg caaacgggcg gtaaagagat taaaagcgcc atcccgatgg gctgtgtgct
      961 gacgctgcca gcaaccggtt cagaatccaa cgcaggcgcg gtgatctccc gtaaaaccac
     1021 aggcgacaag caggcgttcc attctgcaca tgttcagccg gtatttgccg tgctcgatcc
     1081 ggtttatacc tacaccctgc cgccgcgtca ggtggctaac ggcgtagtgg acgcctttgt
     1141 acacaccgtg gaacagtatg ttaccaaacc ggttgatgcc aaaattcagg accgtttcgc
     1201 agaaggcatt ttgctgacgc taatcgaaga tggtccgaaa gccctgaaag agccagaaaa
     1261 ctacgatgtg cgcgccaacg tcatgtgggc ggcgactcag gcgctgaacg gtttgattgg
     1321 cgctggcgta ccgcaggact gggcaacgca tatgctgggc cacgaactga ctgcgatgca
     1381 cggtctggat cacgcgcaaa cactggctat cgtcctgcct gcactgtgga atgaaaaacg
     1441 cgagaccaag cgcgctaagc tgctgcaata tgctgaacgc gtctggaaca tcactgaagg
     1501 ttccgacgat gagcgtattg acgccgcgat tgccgcaacc cgcaatttct ttgagcaatt
     1561 aggcgtgccg acccacctct ccgactacgg tctggacggc agctccatcc cggctttgct
     1621 gaaaaaactg gaagagcacg gcatgaccca actgggcgaa aatcatgaca ttacgttgga
     1681 tgtcagccgc cgtatataca aagtcgcccg ctaagctttt tacgcctcaa actttcgttt
     1741 tcgggcattt cgtccagact taagttcaca acacctcacc ggagcctgct ccggtgagtt
     1801 catataaagg aggaacgtat ggctaatcca accgttatta agctacagga tggcaatgtc
     1861 atgccccagc tgggactggg cgtctggcaa gcaagtaatg aggaagtaat caccgccatt
     1921 caaaaagcgt tagaagtggg ttatcgctcg attgataccg ccgcggccta caagaacgaa
     1981 gaaggtgtcg gcaaagccct gaaaaatgcc tcagtcaaca gagaagaact gttcatcacc
     2041 actaagctgt ggaacgacga ccacaagtgc ccccgcgaag ccctgctcga cagcctgaaa
     2101 aaactccagc ttgattatat cgacctctac ttaatgcact ggcccgttcc cgctatcgac
     2161 cattatgtcg aagcatggaa aggcatgatc gaattgcaaa aagagggatt aatcaaaagc
     2221 atcggcgtgt gcaacttcca gatccatcac ctgcaacgcc tgattgatga aactggcgtg
     2281 acgcctgtga taaaccagat cgaacttcat ccgctgatgc aacaacgcca gctacacgcc
     2341 tggaacgcga cacacaaaat ccagaccgaa tcctggagcc cattagcgca aggaggaaaa
     2401 ggcgttttcg atcagaaagt cattcgcgat ctggcagata aatacggcaa aaccccggcg
     2461 cagattgtta tccgctggca tctggatagc ggcctggtgg tgatcccgaa atcggtcaca
     2521 ccttcacgta ttgccgaaaa ctttgatgtc tgggatttcc gtctcgacaa agacgaaccc
     2581 ggcaaaattg caaaactcga tcagggcaag cgtctcggtc ccgatcctga ccagttcggc
     2641 ggctaacgtg caaattctcc cggtggcggt aatgttccgc taccggactt ttcagaaatc
     2701 atttattccc ctcgcgtccc gcccgttgtt actcttcctt gttcaggaat gccaaatata
     2761 aggacatcat catgcagagc cggaagctct taaaagaaca actcatctat atccgggata
     2821 aacgcaacgg agaggtgaaa aacagatgaa aataatactt ctgtttttag cagccctggc
     2881 aagttttacc gtacacgcgc agcctccttc acagaccgta gaacaaacag tccggcagat
     2941 ttatcagaac tataaatcag atgccagtac gccttatttc ggtgaaaccg gagagcgggc
     3001 gataacttca gcgcgtattc aacaggcgct taccctgaac gacaatctta cactaccggg
     3061 caatattggc tggctggatt atgatccggt ttgtgattgt caggattttg gcgatctggt
     3121 gttagaaagc gtagcgataa cccaaactga cgtcgatcat gccgatgccg ttgtgcgctt
     3181 tcgtatcttt aaagatgata aagaaaagac cacacagaca ctgaaaatgg tggcggaaaa
     3241 tggtcgttgg gtcattgatg atattgtcag caatcatggc agcgtcttac aagcagttaa
     3301 tagcgagaat gaaaaaacgc tggccgcttt agcttcgttg cagaaagaac agccagaatc
     3361 ctttgttgct gaactctttg aacatattgc tgattatagc tggccgtgga cgtgggtggt
     3421 ttccgactct taccgccagg cggttaatgc cttctataaa accaccttca agacggccaa
     3481 taatcccgat gaagatatgc aaatagaacg gcaatttatt tacgacaatc cgatctgttt
     3541 tggcgaagag tcgctatttt cacgcgttga tgaaattcga gtcctggaga aaaccgccga
     3601 ttccgcccgc attcatgttc gttttacgct gaccaatggc aacaatgaag agcaagagct
     3661 ggttttacag cggcgcgaag gtaagtggga aatcgctgat tttatccgcc cgaacagcgg
     3721 cagcctactt aagctgattg aggcaaaaac cgccgccaga ttaaagcaat gagctgaatt
     3781 aaataacaat tagccggaac aataaataaa agggaacact atatgaaaac gattttcacc
     3841 gtgggagctg ttgttctggc aacctgcttg ctcagtggct gcgtcaatga gcaaaaggtc
     3901 aatcaactgg cgagcaatgt acaaacatta aatgccaaaa tcgcccggct tgagcaggat
     3961 atgaaagcac tacgcccaca aatctatgct gccaaatccg aagctaacag agccaatacg
     4021 cgtcttgatg cacaggacta ttttgattgc ctgcgctgct tgcgtatgta cgcagaatga
     4081 taaaaaaatc cccggcagca tgtcagttgc cggggatttt ttttaacgtc caaccgccgc
     4141 tttagggcgt ttcttcgcac cagcattcac cggacgagat tgcgtagacg acgctttttt
     4201 tgccgtagca ggcgtctgac gctgagtcgc catcggcgta tgtttagtca acgccggacg
     4261 ggtattgcgg ttctggcgac gagcttcacg catctcttca atggttggcg caggcactaa
     4321 gcaatcgcga cggctgccaa tcagatgctt tttgcccatc gcttccagcg cctggcggat
     4381 taacggccag tttgccggat cgtggtaacg caacaacgct ttatgcaaac gacgctgttt
     4441 gtcgcccttc ggtacgaaga cgtcttcgct cttataacca atcttcgcca gcggtttttt
     4501 cccggtgtaa tacatggtgg ttgagttcgc cagcggcgat gggtagaagt tctgtacctg
     4561 gtcgagacgg aaacgatgct ttttcagcca cagcgccaaa ttcaccatat cttcatcacg
     4621 cgtaccgggg tgcgcagaga tgaaatacgg gatcagatac tgctctttac ctgcctgttt
     4681 cgagtaggta tcgaacagct ctttaaagcg gtcatagctg cccatgcccg gcttcatcat
     4741 cttcgacaac ggcccttctt cggtatgttc cggagcaatc ttcagataac cgccgacgtg
     4801 atgggtcgcc aactctttga tatagcgcgg atcttccacg gcgatgtcat aacgtacgcc
     4861 tgaggcgata aggatttttt taatgccttt cagatcacgc gcacggcgat agaggttgat
     4921 cgtcggttcg tggttagtgt ccatgtgcgg acaaatatcc ggataaacgc acgacaaacg
     4981 gcgacaagtt tgttcagcgc gtggcgattt gcagcgcaac atatacatgt tggcagttgg
     5041 cccaccgaga tcggaaatca cgcccgtaaa acctggaacg gtgtcgcgga tcgcttcgat
     5101 ctcattaatg agcgaatctt ccgaacggct ctgaataatg cgcccttcgt gctcggtaat
     5161 agaacagaaa gaacagccgc caaagcagcc gcgcataatg ttgaccgaaa aacggatcat
     5221 ttcgtaagcc ggaatacggg cattgccata ggccggatgt ggcacgcgct tgtacggcag
     5281 cgcaaaaacg cagtccatct cttcggtaga aagcgggata gcaggcgggt tgatccacac
     5341 atagcggtca ccgtgttttt gcatcaatgc gcgggcgcag cctgggttgg tttcgtggtg
     5401 cagaatgcgc gaagcatggg cgtacagcac tttatcgccc ttcactttct cgaaagaagg
     5461 cagcaatacg taggtttttt cccacggttt ctggcgcggt ggctgcacga ttacagcttt
     5521 cgcttcctgc tttttcggcg caacaggttt gttatccgca cacggcaaat cttcgccata
     5581 cggatgcggg attgggtcga ttttccctgg ggtatcaaga cgggtggaat ccacaccgct
     5641 ccagccaggc agcgcctctt ttacgataat tgcggtatta cgcacatcgc ggatttcact
     5701 aatcggctcg cccatcgcca gacgatgcgc tacttccacc agcggacgtt caccgttacc
     5761 aaacatcagc atgtcggctt tcgaatctac cagaacggaa cggcgcacgg tatcggacca
     5821 gtaatcataa tgcgcggtac ggcgcaggct agcctcaata ccgccgagga tcaccggcac
     5881 atctttccac gcctctttac aacgctgggt ataaaccagc gtggcgcgat ccggtcgctt
     5941 acccgcgaca ttatctggcg tgtaggcatc atcatgacgt aaacggcgat cggcggtata
     6001 gcggttgatc atcgaatcca tgttgccagc agtaacacca aaaaacagat tcggtttacc
     6061 cagacgcata aagtcgtctt tgctgctcca gtccggctgg gcgatgatcc cgacgcgaaa
     6121 gccctgcgct tccagcatac gaccgcaaat cgccatcccg aagcttgggt gatcgacata
     6181 cgcgtcgcca gtaaccaaaa tgatgtcgca gctatcccag ccaagttgat ccatctcttc
     6241 acgagacatc ggcaaaaacg gtgccggtcc aaaacaggcg gcccagtact gcggccagga
     6301 gaacaggtcg cgatccggtt ggatcaggga gatagagctc attttgcttc cagaaatgat
     6361 aaaaaaataa tcaaaggccg gggattataa gccggaacga aagagaaatc gaaaggtatt
     6421 ccatactcgc cctcctcggg cgagtatgaa gattacggta ccggattgac caacagttgc
     6481 ccaatcgagc cacggtccgc catttccagc gtctgactgt tgaagtagaa cgggaagtgc
     6541 gcccaggaag gctgaccgaa ataaacaagc agctccactt gtccgtcaac ccaaacggta
     6601 tctttccagc ctctgtcttc cgggaacggc atcgcgccat tcacgttacg gatctggaac
     6661 attacgcctt caatatggaa cgcttgcggc tcgtccgcgc gtaccgtcca gcgttcccac
     6721 gttccttgct gcgcggtgac atcaatacgg ttgacgtccc acagctgtcc attaataccc
     6781 gggtcatcac ccagactgat atcgcgactg cgaattggcg aaccagccat gatttcagtt
     6841 ggcagcaagc gcatcggaag actgtctgtg accagcggca gaaggccggt tgggcgcagc
     6901 gttagcacca aggtagaaac cagaatactg gatggctcaa agaagccgcg aatacgatca
     6961 acaatgctcg ccgcttcgcc acaggtgatc gacacttcat cgccgttgct catatccacc
     7021 agaatctcgc ggcgctcgcc cggtgccagc gaaagttgct tcaccgatac aggagcaggc
     7081 aggaatccct gatcgccaga aatcacatgt aacgggcgac catcgctcat ctgtagttga
     7141 tagcgacgag agttcgacgc gttcagcagt cgcaagcgca cccagccacg cgagacttca
     7201 acgtacgggc tttgtacacc gttaaccagc agcgtatcac caacaaagcc gccgcttccc
     7261 ggttcgttgt attctggcgt accaaagtta tccagccgtt tatcctggat aatgaccgga
     7321 aaatcatcca caccataatg gttggggata ggcagcgact tgctgacttc atcttccacc
     7381 agccacattc cggcaaggcc gttatagacc tgctgagccg tgcggttggg agtattggcg
     7441 tgataccaca gagtagctgc gttctggcga ataggcagta cgggtgccca gtcagcgttt
     7501 ggcgacatca tccgtgccgg accgcccatc agcgggcctg gtacctgtag cccggccacc
     7561 gtcattgaga cattttctgt caggcggttg ctgtaaataa gcttaacatc gtcgcccttc
     7621 cagacgcgga tagtcggccc caggtaacga ccattgattc cccagaccga cgcgcgtgtc
     7681 cctggcgtaa atgaccagtg cgcacgttgt acagtcataa acagcggttg cccacggcga
     7741 gattcaagta gcggcggaac gggtagcggt tgctgttgcc cggctgcgct ggccttcagg
     7801 ggaacagcgc ctgcacaaag tgcaatcccc gatgcctgaa tgaactgacg ccgactgagt
     7861 gacatatttg ctccatgtaa aactggctaa agtaataccg tgcaggaaag tgttccccgc
     7921 aaaaatacag attgccgact taaacttttc cggcggcttc gcgttctgcg acttctttat
     7981 cgagctcggc gattttttgt tccattatcg aacgacaatg ggcagccagc tcacgaacct
     8041 gatctttgcc atactgactg acgtcaattg gcggcagcat ttcgacaatc accagaccgt
     8101 tgtgcagtcg attaagatta atcttattcg aagttgtaga gacgcacacg ggaataatcg
     8161 ggacgcccgc cgcaattgcc gcgtgaaatg ctccagtctt gaacggtagc aggccgcgac
     8221 cacggctgcg ggttccttcc gggaacatcc agatggaaat acggcgtttt ttgaaatgat
     8281 tcacgacttc cgcaatggtg ccgtgagctt tagtgcgatt gtttctgtcg atcaataagt
     8341 tgccggttaa ccagtacaac tgcccgaaga aggggatcca cagcaagctc tttttaccta
     8401 ccgtcaccgt cggcggttgc acaatgttcg atgctgtcac catgtcatag ttgttctggt
     8461 ggttagcgat atagatagca ttgccgtagc tttcagcatc tgcaggtttg cggcactcaa
     8521 ctttcaggcc gaacagtggc gcaagacggc caaacatatg cccaaaggtg gccacatgtt
     8581 tcgggttacg cgggctgaaa aggcagtaaa tggagccgaa tacacagact aagatgctgt
     8641 aaatcacggt aataataaga cgaaagatat atagcataac accctctgaa ggtctgagag
     8701 actggcattg tacgtcacct gaaatcaggt tagggcattg ttaaaagccg ctctctggaa
     8761 aagcgtattg ttaatcgcaa cgcatgaata aacaacggtt tcaagggaat ttttatcgtt
     8821 tccgggggtt gaatacgctg ccggatgacg acttaacgtt tcatccggcg ttccttgcta
     8881 gcgggaggaa acagcgccct ccccggcata ttactcttcg ctatcaccgc tgctggcacg
     8941 gcgaggagag tcgatctcaa cacgatcgat acgctgcaaa ccgcgcatca acgtaccgcg
     9001 gcgtccacgt tcgccagtga ctttctgtag ctcttccgga cgcagtttaa ttttgcgttt
     9061 cccaacatga atggtcagcg tgctttgcgg cggcagaacg tacagttgcg ccagaccatc
     9121 ctcgccacgt gcggcttctg ccgatggaat gttgataatc ttgttgcctt tgcccttcga
     9181 cagctgcggc agatcgctta ccgggaacat caacatacgg cctgcctgag tgattgccag
     9241 cagcatatcg gaagcatctt caatcaccac cggcggcata acatgggcat tttccggtaa
     9301 ggtgatcaaa gccttacccg cacggttacg cgccaccaga tcgttaaagg tgcagacgaa
     9361 accgtaaccc gcatcggaag ccatcagcag tttctgatcg tcgctttcca tcagcatatg
     9421 gtcaacggtc gccccaggcg gcaacgttaa tttgccggtg agtggttcgc cctgaccacg
     9481 cgccgacggc agcgtaatcg ggtcgatggc atagctacga ccggtggaat caacaaacac
     9541 taccggttgg ttactcttac ctttcaccgc cgctttgaag ctatcacccg ctttataatt
     9601 caggcccggc gcgtcgatat catggccttt agcgctgcgt acccagccca tctgcgacag
     9661 cacaatggtg acaggttcag acggcagcat gtcgtgctcg ctcatagctt tcgcttcttc
     9721 gcgttcctgc aacggcgaac gacgatcgtc accgtaggct tgcgcgtctg cctgcagttc
     9781 tttcttcagc aggttattca ttttacgctc ggaagccaaa atgccctgca actggtcgcg
     9841 ctctttttcc agctcactct gctcaccgcg aatcttcatc tcttccagtt tggcaagatg
     9901 acgcagtttc agttcgagga tcgcttccgc ctgggtttcc gtaaggccaa accgcgacat
     9961 cagcgccggt ttcggttcat cttcgttacg aatgatctca atcacttcgt cgatgttgag
    10021 aaacgccacc agcaaacctt ccaggatatg caggcgcttg aggactttct ccagacgata
    10081 gttcagtcgg cggcgcacgg tatcgcgacg gaacaccagc cattcggaga ggatttccag
    10141 caggtttttc accgccggac gaccatccag accgatcata ttgaggttaa tgcgatagct
    10201 cttttccaga tcggtggtag cgaagaggtg gttcatcacc tgatccatat ccacgcggtt
    10261 ggaacgcggc acaatcacca gacgggtcgg gttctcgtgg tcagattcat cgcgcagatc
    10321 gtcaaccatc ggcagctttt tgttgcgcat ttgcgcagca atttgctcca gtacgcgcgc
    10381 acctgaaacc tgatgcggta atgcgctgat aaccaccgcg ccatcttctt tcttccacac
    10441 cgcgcgcata cgcactgaac cacgtccgtt ctcgtagatt ttacgaatct cggcgcgcga
    10501 agtgataatt tccgcttcag tcggataatc cggcccctgc acgatatcca gcagctgatc
    10561 gagcgtggtt ttcggctggt cgattaatgc gattgccgcc tgagctactt cacgcaggtt
    10621 atgcggtgga atatcggtcg ccatgccgac ggcaataccg gtggtgccgt taagcaaaat
    10681 gtttggcaga cgggcaggta gcattttcgg ctcctgcaaa gtgccgtcga agtttggcac
    10741 ccagtcagcc gtcccctgcc ccagctcgct caatagcagc tcggaatatt tcgacaaccg
    10801 ggattcggtg taacgcattg ccgcgaacga tttcggatcg tccggcgcgc cccagttccc
    10861 ctgaccatca accagcggat aacggtaaga gaacggctgc gccatcagga ccatcgcttc
    10921 ataacaggcg ctatcgccgt gcggatggta tttacccagt acgtcaccga cagtacgggc
    10981 cgatttttta aatttggctc tggcattcag gcccagttca gacatcgcat acacaatgcg
    11041 gcgctgaaca ggtttcagac catcaccaat aaacggcaac gcacggtcca tgatcacgta
    11101 catggagtag tttaagtagg cgttttccgt aaattcatgt agcgcaaggc gctctgccat
    11161 atcgctcatt aattctgatt cctcaactta ttcgccagcc ttcaaagggg caatattgcc
    11221 ggagatacta cattatctgc cgcgtcgagt cacaggagat cagcgtgaat agcgccgccg
    11281 gacgtgctcc gacggcgata aaggcatcaa tgtgccttga tatacaactc tttgctgtag
    11341 acttgatcgg catagagggc agcacaaggt tgcgcccatg ccacaccact cggcatgggg
    11401 gtccgctccg ggcgattcga gaagttgagg ttatgcgagt ccagggaagc ctaaagcatc
    11461 aaagtagtcg actgtaagta tacggttcag cccagtttag ctgtgtctcc accagtgccc
    11521 cgcgcctcct gccataagtg cggcttgctc atggataccc ccgagacggc ttacggcgca
    11581 ccggataata aatttcgcgt atacatggta ttccttaccg gagaaattat aagcccaaca
    11641 acaatacgcc tgtgacattg ttcgctcaaa cttatcgcga gtgatttggc tcacactccg
    11701 gtaaagattc cccatcacgc caacggcaca taaacatccg tttccagtcg ctgctctgtc
    11761 accccttcgg caagattggt gtagtgaaac agaatcggtg ctttacgcat tttctcgccg
    11821 cttgcaggca gccagtggcg aataatgccc catatcgtgt gtgaaatatc gtccagctcg
    11881 ccaacgtggc gggccacggc ataacgtcca ccggtaagtt caccattgct cacaccataa
    11941 cgattatcgg gaatcggttc gctaacgctg ccgcagatat caaagcgaaa cgcttccggt
    12001 gcggtggtgg cagggtcgtc ccaggcgacg ccaaaagtct ggctttggtt aacaggagaa
    12061 agccccgtct ctttacgcca catgataaat ttcgccacac tgtagttgag caactcaggg
    12121 ctacagcggt gcggcaacat cgccacaggg attgatggga aatcgataat gtttacatcc
    12181 agtgtcaggt ttgtcatttt gcctccctgc gaaattattc ccgggaacgc gtcccgggag
    12241 cggtaacaat tacggattta ctttgcggat ctgtttgacg tcaatttcaa cagaattcca
    12301 gtctttatcg acttcaccct gaatctcaac cgtatctttc ggcgtcaccg tcacaccgtt
    12361 ccagcgtttg tgatcgatat caacattgat agtaccgctg gcatctttaa acacgtagag
    12421 atcgtcagag atgcgttcaa cgatattgcc gcgcagggtt acccaggtgt cgtcacgcag
    12481 ggattttacg ctttctacag tcgttacgct gccgttcggc ccctgaaatc ctccggcctg
    12541 actttgcgtt gccgatgggc cagaaaaacc gccctgctct gctgccatca ccggtgcact
    12601 gcacagggcc attactgcga ttactgctgc gaattttttc atgtttatta ctccctttaa
    12661 tgtctgtttc cgagcattta acaagatagt ccttaacaac ttcttaaggg aaaaaaataa
    12721 aatttaatgc tgtacagagc gcgttacaac acggtttact ggcagcaaat acggttatcg
    12781 cagggatgaa aaaatgcgaa ttttactgat agaagatgac atgctgattg gtgacggcat
    12841 caaaactggc cttagtaaaa tgggttttag tgtcgactgg tttacacaag gtcgtcaggg
    12901 aaaagaggcg ctttatagcg caccttatga tgcggtgatc ctggatttaa ccttaccagg
    12961 catggatggt cgcgatattt tgcgcgaatg gcgagagaaa ggtcagcgtg agccggtact
    13021 gaacctgacc gcgcgtgatg cgttagcgga acgtgtagaa gggctgcgtc tgggagctga
    13081 cgattatctg tgtaaacctt ttgcgttgat agaagtcgcc gccaggctgg aggctctgat
    13141 gcgccgaacc aacggccagg ccagcaacga gctgcgccac ggtaacgtca tgctcgaccc
    13201 cggcaaacgt atcgccacgc tggctggcga acccttaaca ctgaaaccaa aagaatttgc
    13261 cctgctggaa ttactgatgc gtaacgctgg tcgggtactg ccgcgcaaac tgattgaaga
    13321 gaaactgtat acctgggacg aagaggtcac cagtaatgcc gttgaagtgc atgtgcatca
    13381 tctgcgacgc aaactcagta gtgattttat tcgtaccgtg catggtattg gctacacatt
    13441 aggtgagaaa tgaaatttac ccaacgtctt agtctgcgcg tcaggctgac gctaatcttt
    13501 ttaattctgg cctcggtgac ctggctgctt tccagctttg tcgcctggaa acaaacaacg
    13561 gataacgtcg atgaattgtt cgacacccaa ctgatgctgt ttgccaaacg gttaagtacg
    13621 cccgatctca acgaaatcaa cgcggcggat cgcatggcac agacgccaaa taaattaaaa
    13681 cacggtcatg ttgatgacga tgcgctgacc tttgccatct ttacccacga cggcagaatg
    13741 gtccttaatg atggcgataa cggagaagat attccctata gctatcaacg ggaaggtttt
    13801 gctgacgggc aactgatcgg tgaagacgat ccttggcgtt ttgtctggat gacctcacct
    13861 gatggcaaat atcgcatcgt tgttggccag gaatgggaat accgtgaaga catggcgctg
    13921 gcgattgttg ccgggcaatt gatcccgtgg ctgctcgcac tgccgattat gttaatcatc
    13981 atgatggtac tactgggtcg tgaactcgcg ccgctgaaca aactggcgct ggcactacgt
    14041 atgcgtgacc ctgactcgga aaaaccacta aacgcgactg gcgtacccag cgaagtgcgg
    14101 ccactggttg agtcgctaaa tcaactgttc gcccgcacac atgcgatgat ggttcgagaa
    14161 cgacgcttta cctccgatgc agctcacgaa ctacgtagcc cgttaacagc gctgaaagtg
    14221 caaaccgaag ttgcgcagct ctctgacgat gatccgcagg cgcggaaaaa agcactgctc
    14281 caattacatt ccgggatcga tcgcgctact cgtctggttg atcaactgct cacgctatcg
    14341 cggctggact cactggataa ccttcaggac gtcgcggaga tcccgcttga agatctccta
    14401 caatcgtcgg tgatggatat ttaccacacg gcgcagcagg cgaaaattga cgtgcgactg
    14461 acactcaatg cccacggcat caaacgcacc ggacaaccgc tattgctaag tttgttggtg
    14521 cgaaatttgc tggataacgc cgtgcgctac agtccacagg gcagcgtgat agacgtcacg
    14581 ctgaatgctg ataatttcat cgtgagggat aacggccccg gtgtgacacc agaggcactg
    14641 gcgcgaattg gcgaacgctt ctatcgccca cccggacaaa ccgctaccgg cagtggactt
    14701 gggctatcga ttgtccagcg aatcgccaag ctgcatgaca tgaatgttga gtttgggaat
    14761 gcggaacaag gtggatttga ggcgaaggta agctggtaac ttctctgtct atgagagccg
    14821 ttaaaacgac tctcatagat tttactaata gcaaaatata aaccgtcccc aaaaaagcca
    14881 ccaaccacaa accagacaaa taacgagcga gaatacatat agccaaaact cgaacatttc
    14941 ccgcataaag agttttctta agataagaat aataagtggc gtaagaagaa aaaatgctgc
    15001 aatcattgct tgcaatatac gatcttcgcc tgaaacggaa ggtatactgc aaatattgcc
    15061 tcccgactca agcatccagt catacttatt gaaagcaaaa aaagaatcta ttaaccaata
    15121 agtaagcatt atatatagca atgcttcaaa tattgtttta attttctgct ttaacatata
    15181 tcagtacgct cataaaaatc catttataat tatcaacatg acacgttgat attttcatct
    15241 tttacttatt caaagccata ttttcaatcc ccaaaaaaac cgaaccaaac gtgtaaatta
    15301 ttccggtacg agtaagaatt atgaggaatg gctatcagta ttgtcatttt cagaaaatat
    15361 ttatcctgca tcggtgagtc agagctagat cagacttttg ctaaattcgc aaaagacttt
    15421 gcacattttg ctaatttcac cgtaccgctc tgtgacgtac tatagtcgac aaacgtctca
    15481 ccttgaggtt aaaaaatgag caacatcctg attatcaacg gcgcgaaaaa attcgcccac
    15541 tccaatggtc aactgaacga caccctgacc gaagtcgcgg atggcacact gcgcgacctc
    15601 gggcatgatg tccgcatcgt tcgcgccgac agcgactaca atgtcaaagc ggaagtacaa
    15661 aactttctct gggctgatgt ggtgatctgg cagatgccag gctggtggat gggcgcgccg
    15721 tggacagtga aaaaatatat tgatgatgta ttcaccgaag gtcacgggac tctgtatgcc
    15781 agcgatggtc gtacccgcaa agatccgtcg aaaaaatacg gttccggcgg cctggtacag
    15841 ggcaaaaaat atatgctttc tctaacctgg aacgcaccaa tggaagcctt caccgaaaaa
    15901 gatcagttct tccacggcgt tggcgttgac ggtgtgtatc tgccgttcca taaagcaaac
    15961 caattcctcg gtatggaacc gctgccgaca tttatcgcta atgacgtgat aaaaatgcct
    16021 gatgttcccc gctatactga agaatatcgc aagcatcttg tggaaatttt tggttaacta
    16081 gagctcaggc tttagaagga gttaaccatg cttaccgtaa tcgcagaaat ccgtactcgt
    16141 cctggtcaac atcaccgtca ggcggtattg gatcagtttg ctaaaatcgt tccaaccgta
    16201 ctgaaagaag aaggttgcca cggctatgcg ccaatggtgg attgcgctgc tggcgtgagt
    16261 ttccagtcta tggcaccgga ttctatcgtg atgattgagc agtgggaaag catcgcgcac
    16321 cttgaagcgc atctgcaaac cccgcacatg aaggcgtaca gcaaagccgt aaaaggcgac
    16381 gtgctggaga tgaatatccg tattctgcag ccagggattt aatcctgcct tgtttgcccg
    16441 gccatcctga ccgggcaatg ttctttcctt taaacctcaa tctccgccat gtcgcctttc
    16501 tcttgcaacc agttgcggcg atcttccgag cgtttcttcg ccagcagcat atccatcatc
    16561 gcgtcagtac gctgatcgtc ttcatcatcg atagtcaact gcaccagacg gcgagtgttc
    16621 ggatcaagcg tggtttcacg caattgcatc gggttcattt cccccagacc tttaaaacgc
    16681 tggacgttcg gcttgccttt cttgcgtttt aattgctcaa gtacgccctc tttctcttct
    16741 tccgtcagcg cgtaataaac ctctttcccg agatcaatac ggtagagcgg tggcagtgcg
    16801 acgtaaacgt gaccgtgttt caccaacgcg cggaagtgtt ttacgaacaa agcgcagagc
    16861 agcgtggcaa tgtgcagacc atcagagtcc gcatccgcga ggatacagat tttgccataa
    16921 cgaagctggc tcagatcgtc gctgtcagga tcgataccga tcgctaccga aatatcatgc
    16981 acttcctgcg aggccagcac ttcgtcggaa gaaacttccc aggtgttaag gatcttacct
    17041 ttcagcggca tgatcgcctg atattcacga tcgcgcgcct gcttggcaga tccgcctgcg
    17101 gagtcacctt ccacgaggaa cagttcggta cggttaaggt cctgcgcggt acaatcagcc
    17161 agtttgccag gcagcgccgg gccgttggtc agctttttac gcaccacttt tttagccgca
    17221 cgcatacgac gctgggcgct ggaaatcgcc atctctgcca gcaactccgc cgcctgaacg
    17281 ttctggttca gccacaggat gaaggcatct ttcaccacgc ccgaaacgaa tgccgcacac
    17341 tgacgcgaag agagacgctc tttggtctgc ccggcaaact gcggatcctg cattttaacc
    17401 gacagaacat aggcgcagcg atcccagata tcttccgccg acagcttcac gccgcgcggc
    17461 agaatgttgc ggtattcaca gaactcacgc atcgcgtcca acaggccctg acgcagacca
    17521 ttaacatggg taccgccctg catcgttggg ataaggttga cgtagctttc ggtcagcagt
    17581 tcaccgcctt ccggcagcca cagtagcgcc cagtccacag cttcagtatc gccagcgaaa
    17641 ttaccgataa acggtttttc tggcagcgtc ggtaaaccgt ttaccgcttc cgccaggtaa
    17701 tcattcagac cgtcctgata gcaccagcgc tgttcggtgt tgttgatctc atctttaaag
    17761 gtaatctcaa cgccggggca cagtactgct ttggccttca gcacatgcgt caggcgtgaa
    17821 acagaaaaac gcgggctgtc aaagaaggtt tcatccggcc agaagtgcac gctggtaccg
    17881 gtattgcgtt taccgcaagt gccgacaacc tgtaaatcct gcaccttttc gccattttca
    17941 aaggcgatgt tataaacctg accatcgcgg cgcacgttaa cttctacgcg cttcgacaag
    18001 gcgttaacta ccgaaatccc cacgccatgc aggccgccag agaactggta atttttgtta
    18061 gagaatttac cgcctgcatg cagacggcaa agaatcagtt caaccgccgg tactccttct
    18121 tccgggtgaa tatccaccgg catcccgcgc ccatcgtcaa tcacttctaa cgactggtca
    18181 gcatgtaaga ttacatccac gcgttttgcg tgacccgcca gtgcttcatc gacactgtta
    18241 tcaataactt cttgccccaa atggttaggg cgagtggtat cggtatacat ccccggacgg
    18301 cggcgaaccg gctcaagccc ggtgagtacc tcaatggcat cagcgttata agtttgcgtc
    18361 atggtttaag ttagcaattc gagttgatcg tcagagatgg tgcagaccaa gaaaatcgac
    18421 gatcgggttg aaataatctt cgaagcccgt gaatgcgtgg ttgccgcctt ctatgacagt
    18481 ctggcggcag gaagcgtagt acgccaccgc ctggcggtaa tccagcactt catctcccgt
    18541 ctgttgcagc agccagatca aatccggcgc ttccagcggg tcaatctgca tgactttgag
    18601 atcgtaaata tggcgtgact ctagcacata ttgctgcccg gtgtaggggt tctcgttctg
    18661 accgagatag tccgtcagca attcaaacgg gcgtaccgcc gggtttacca ccactgcggg
    18721 cagcataaaa cattgcgaca accaggtggc gtaatatccc cccagtgacg aaccgacaat
    18781 acccagcgaa tcgccgccat gttccaggac aatggattcc agcagttctg ccgcgtcgga
    18841 aggatacggc ggcaactgcg gaatgatcat ctcaacgtca gggtgatgtt ccgccagcca
    18901 gttttttaac aagctcgctt ttgcagagcg cggcgagctg ttgaaaccgt gtaaataaag
    18961 aagcgtagac atcagtagcc ttctgaagcg gtatcaggtt ggaaacgtgt gtccgccagg
    19021 cgatgcacct cggtggtcag cgtgccatca gcatgtaact cgagagtacg ccagccgggc
    19081 gcgatagtat ccagcgtaaa gttggaacag tgcggcttaa actgcacaca ggtcgacggc
    19141 gttgccagca ggcggcgacc gttccagtcg agatccagct cctgatgaat atgaccgcac
    19201 agcaagtatt tgacgtgcgg aaacttcgcc agcacggtat ccagttcgcc cgcgttacgc
    19261 agactgtgtt gatcgagcca actacaaccc gcaggtagcg gatgatgatg cagcagcagc
    19321 aacgtatggc gttctggcgc atcggccagt ttacgttcca gccactcaag ctgaaactcg
    19381 ctcagctcac cgtgcggcac gccaaacacc tggctatcca gcaacaggat ttgccattgc
    19441 tcaccaataa acacccgctt cgccggggag atacccgcat cctgtaacgc gctgtacatc
    19501 gcgggctgga aatcgtggtt gcccggcagc cagacgcagg gcgcacgaaa acttgcgatg
    19561 ccttcagcga aatgctgata ggccgcagag gattgatcct gcgctaaatc acctgtcgcg
    19621 acaatcaggt cgaattcgtg ctggtgtgga cgaatcgcct ccagcaccgc ctggtagctt
    19681 tcccaggtgt ttacccctaa cagggcttcg tgcttttgtg caaacaggtg agtatcggta
    19741 atttgtaaaa tccttcctct ggcctcacca gccagaggaa gggttaacag gctttccaaa
    19801 tggtgtcctt aggtttcacg acgctagtaa accggaatcg ccatcgctcc atgtgctaaa
    19861 cagtatcgca accagtccgc taaaaactgg ttaatttgat gcttttcgtc gcgttgatgc
    19921 aactttttat taggataatc ataccgcgct ttgaagcgaa aaatctgctg gcttgaacac
    19981 acttcagcca ccatcgcgtc atgatacaga cgcaccgtca ttgacggaag gctccagtaa
    20041 ctgatcgcgg gcgcagtctg ttctattgtc accagggtag tgtatcgggt cgattccaca
    20101 atcgtcagcc gatattgtgc gtttgccacc tgatagctta cagtttcgcc gggtgcgtca
    20161 ttgcgcggta acaaacggcg caattgtgaa aagttcatct cgcacaggcg catcatttca
    20221 ggaaagtcag gtgtgtaacg cttcatttat gcccactcat tttttaacgc ttgatgatgc
    20281 agctgcagcc attgcaaagc gatgaccgac gctgcgttgt cgattttccc ctcttctacc
    20341 cactggtatg cctgttcccg gcttaccaca tgaacgcgaa tatcttcgtt ttcatcagcc
    20401 agaccatgaa taccgcttgc ggtcgtggcg tccacttcgc ccaccataat tgacgaacgc
    20461 tcactggtgc cccccgggct tgccaggaaa cttaacaccg gtttggtccg tttgactatc
    20521 agtcccgcct cttcaatcgc ttcgcgacgg gcaacatctt ccacactttc accctcttca
    20581 atcatcccgg caaccatctc cagtagccag ggggtttcgc tggtgtcgta cgcggcaatc
    20641 cgaatctgct caatcagcac aacttcatca cgcactgggt caaagggtag caagactgcg
    20701 gcgtgaccgc gctcaaaaat ttcccgccgt acctcatgac tcatttgccc gttgaataga
    20761 cgatgacgaa atctataaag atctaatgaa aaaaagccgc gataaagtgt ttctcgtgca
    20821 ataatttcta catcgttttt gccaaatgta acgggcaggt tgtctggctt aagcattgtt
    20881 aatgtcctgg cactaatagt gaattaaatg tgaatttcag cgacgtttga ctgccgtttg
    20941 agcagtcata tgttaaattg aggcacatta acgccctatg gcacgtaacg ccaacctttt
    21001 gcggtagcgg cttctgctag aatccgcaat aattttacag tttgatcgcg ctaaatactg
    21061 cttcaccaca aggaatgcaa atgaagaaat tgctccccat tcttatcggc ctgagccttt
    21121 ctgggttcag ttcgttgagc caggccgaga acctgatgca agtttatcag caagcacgcc
    21181 ttagtaaccc ggaattgcgt aagtctgccg ccgatcgtga tgctgccttt gaaaaaatta
    21241 atgaagcgcg cagtccatta ctgccacagc taggtttagg tgcagattac acctatagca
    21301 acggctaccg cgacgcgaac ggcatcaact cgaacgcgac cagtgcgtcc ctgcagttaa
    21361 ctcaatccat ttttgatatg tcgaaatggc gtgcgttaac gctgcaggaa aaagcagcag
    21421 ggattcagga cgtcacatat cagaccgatc agcaaacctt gatcctcaac accgcgaccg
    21481 cttatttcaa cgtgttgaat gctattgacg ttctttccta tacacaggca caaaaagaag
    21541 cgatctaccg tcaattagat caaaccaccc aacgttttaa cgtgggcctg gtagcgatca
    21601 ccgacgtgca gaacgcccgc gcgcagtacg ataccgtgct ggcgaacgaa gtgaccgcac
    21661 gtaataacct tgataacgcg gtagagcagc tgcgccagat caccggtaac tactatccgg
    21721 aactggcggc gctgaatgtc gaaaacttta aaaccgacaa accacagccg gttaacgcgc
    21781 tgctgaaaga agccgaaaaa cgcaacctgt cgctgttaca ggcacgcttg agccaggacc
    21841 tggcgcgcga gcaaattcgc caggcgcagg atggtcactt accgactctg gatttaacgg
    21901 cttctaccgg gatttctgac acctcttata gcggttcgaa aacccgtggt gccgctggta
    21961 cccagtatga cgatagcaat atgggccaga acaaagttgg cctgagcttc tcgctgccga
    22021 tttatcaggg cggaatggtt aactcgcagg tgaaacaggc acagtacaac tttgttggtg
    22081 ccagtgagca actggaaagc gcacatcgta gcgtcgtgca aaccgtacgt tcctccttca
    22141 acaacattaa tgcttctatc agtagtatta acgcctacaa acaagccgta gtttccgcgc
    22201 aaagctcatt agacgcgatg gaagcgggct actcggtcgg tacgcgtacc attgttgatg
    22261 tgttggatgc gaccaccacg ctgtacaacg ccaagcaaga gctggcgaat gcgcgttata
    22321 actacctgat taatcagctg aatattaagt cagccctggg tacgttgaac gagcaggatt
    22381 tgctggcact gaacaatgcg ctgagcaaac cggtttccac taatccggaa aacgttgccc
    22441 cgcaaacgcc ggaacagaat gctattgctg atggttatgc gcctgatagc ccggcacccg
    22501 tcgttcagca aacatccgca cgcactacca ccagtaacgg tcataaccct ttccgtaact
    22561 gatgacgacg acggggcttc ggccccgtct gaacgtaagg caacgtaaag atacgggtta
    22621 tctgccgcat tcttccccct tctcgcttca atttcgacca gccatcctct attctgatgg
    22681 gtatttacca ctggtcccgg aagacaaaaa tgaaacggac aaaatccata cgccacgcat
    22741 cgttccgcaa aaactggagc gcacgccatc tgacaccagt cgctctcgcg gttgccactg
    22801 tttttatgct ggctggctgt gaaaagagtg atgaaacagt gtctctctat caaaatgctg
    22861 acgactgttc agctgcaaac ccaggcaaaa gcgccgaatg taccaccgcg tacaacaatg
    22921 cgctgaaaga agccgaacgt actgcgccga aatacgccac ccgtgaagac tgtgttgctg
    22981 aatttggtga aggtcagtgt cagcaagcac cagcccaggc tggcatggca ccagaaaacc
    23041 aggcgcaggc tcagcaatcc agcgggagtt tctggatgcc gctgatggcc ggttacatga
    23101 tggggcgtct gatgggcggc ggcgcgggat ttgcacagca gccgctgttc tcctcgaaaa
    23161 acccggccag tccggcttac ggtaaatata ccgacgcgac gggtaaaaac tatggcgcag
    23221 cccagccagg ccgcaccatg accgtaccga agacggcaat ggcaccaaaa ccggcgacca
    23281 ccactaccgt tacccgtggc ggttttggtg aatctgttgc caaacaaagc actatgcagc
    23341 gtagcgcaac cggcacctct tctcgttcaa tgggtggctg ataccgatgg aaagagtcag
    23401 tattaccgag cgcccggact ggcgtgagaa agcccacgaa tacggtttca attttcacac
    23461 catgtacggc gagccgtact ggtgtgaaga tgcttactac aagttgaccc tcgcccaggt
    23521 tgaaaagctg gaagaagtca ccgccgaact gcaccagatg tgcctgaaag tggtggaaaa
    23581 agtgatcgcc agcgatgagc tgatgaccaa attccgcata ccaaaacaca cctggagttt
    23641 tgtgcgtcaa tcatggctga ctcaccagcc atcgctttat tcgcgtcttg atctggcgtg
    23701 ggatggcact ggcgaaccta aacttctgga aaataacgcc gatacgccaa cgtcactata
    23761 cgaggcggcg ttctttcagt ggatctggct ggaagatcag cttaacgccg gtaacttgcc
    23821 ggagggcagc gaccagttta acagtctgca agaaaaactg atcgatcgct tcgttgagct
    23881 gcgtgaacag tgtggcttcc agttgctgca tctcacctgc tgtcgcgaca cggtggaaga
    23941 tcgcggaacc attcagtatt tgcaggactg cgcaaccgaa gctgaaatcg ccaccgagtt
    24001 cctctacatc gacgagatag gtttaggcga aaaaggccag ttcacggatt tacaggatca
    24061 ggtgatttct aacctgttca agctttatcc gtgggaattt atgctgcgcg aaatgttctc
    24121 caccaagctg gaagatgctg gcgtacgctg gctggaaccg gcgtggaaga gtattatctc
    24181 caacaaggca cttctaccgc tactgtggga gatgttcccg aatcacccga acctgctgcc
    24241 cgcttatttt gcggaagatg atcatccgca aatggaaaaa tatgtggtta aaccgatctt
    24301 ctcccgtgaa ggcgcaaacg tgtcgatcat tgagaacggc aaaaccattg aagcagcgga
    24361 aggtccgtat ggcgaagaag ggatgattgt tcagcaattc cacccgttac cgaaattcgg
    24421 cgacagctat atgctgattg gtagctggct ggtgaacgat caacctgccg gaattggcat
    24481 tcgtgaagac cgtgcgttga tcacccagga tatgtctcgg ttttatccac atatttttgt
    24541 tgaataagcc acgataccgg atggcactcg ccatccggta attgttagcc tatctgcacc
    24601 gacagcatac ttaaactgcc catttctata ccatcgaccg gaatggtaat tggctcctgc
    24661 ccatcccaca cacctaacac atacaacaac ggcagatagt gctctggcgt tgggttcgat
    24721 aacgcgccac cttcatggtc gagataattc accagaggat gctgctctac cggcccttgc
    24781 cacgtcagat tcgctttcac atactcatta aacgacgtcg cccacggata cggtgaacta
    24841 tcaccgtgcc acttcactgt gcgcaggtta tgcaccacgt taccgctggc gaccaacatt
    24901 attccttcat cacgcaacgc cgcgagtttg cgccccattt cgaaatgcca ggcggcaggt
    24961 ttgctactgt cgatactcaa ctgcaccata gggatatcgg cgtcaggata catcttaatc
    25021 agcacgcccc acgagccgtg gtcaaagccc caggcttctt tatccagcgt caccgggatc
    25081 ggcgctaaca gctcaaccag acgctgtgcc agcgcaggcg aacccggagc aggataatgc
    25141 gtatcgtaca gcgcctgcgg gaagccacca aagtcatgaa tcgtgggcgg cgtctccatc
    25201 gcggtcactc ctgttccacg ggtaaaccag tgagccgaaa ccaccacaat cgcttgcggg
    25261 cgtggcaatg tcatccccaa cttctgccag ctgcgggtat acaaattatc ttccagcacg
    25321 ttcatcggac taccgtgacc taaaaacaat gctggcatac gtgttgaaga catgatgata
    25381 tccttaacta aaggtgtcat tttgatatcc tcacaatacg cttgttcggc ggagtaagaa
    25441 cccggataac aatgatgatg atcatcagtt attttgacga tctgcctgaa ggtgaagatt
    25501 tataaggagt tgtcgatgtc agtacctctc attctgacca tactggcggg ggcagccacg
    25561 tttattggcg cgtttctcgg cgttctcgga caaaaaccct cgaaccgctt actggcgttt
    25621 tcgctaggtt ttgcggcggg gatcatgttg ctcatctcat taatggaaat gcttcctgcc
    25681 gcactagcag ctgaaggaat gtcgccggtg ttgggttatg gaatgtttat cttcggtctg
    25741 cttggctact ttggcctgga ccgcatgttg ccgcatgcgc atccgcagga tttaatgcaa
    25801 aaatcggtgc agctgttgcc aaaatcgatc aagcgcacag ccattctgct cactctcggc
    25861 atcagtctgc ataacttccc ggaagggatt gccacctttg tcacggcgag cagcaacctg
    25921 gagctgggat ttggcatcgc actggccgtc gcgttgcaca atatccctga aggtctggca
    25981 gtggcaggcc cggtttatgc ggcaacgggg tctaaacgca ccgcgattct gtgggcgggg
    26041 atttctggac tagcagaaat tcttggtggt gtgctggcgt ggttaatcct cggtagcatg
    26101 atttccccgg tggtcatggc ggcaatcatg gcggcggttg caggaattat ggtggcgctc
    26161 tcggttgatg aattaatgcc gctcgccaaa gagattgacc ctaataataa ccccagctat
    26221 ggcgtactat gtggaatgtc agtgatggga ttcagtttag tgctgctaca aacggcggga
    26281 attggttaaa aacaacatca gaagttgtcc agcacatgga caacttctgc cagcttcacg
    26341 aaagccttat tctacctttt cggctgtctc ttctctcgta ctgtttaagt atttgttccg
    26401 cgcttcaagc agtgcgtctc tgtcctggcg aaacgcccga caataacgtt tcaaatgcag
    26461 gaaataaccc accacaacca gagcaatcaa tacaatccag taccaggcga taaacatcct
    26521 tttaccctta atatttaaca atactaaaca tttagcgcat aaatttcaca tatccttttt
    26581 cggatatatt catatggtcg cataataacg gaacaattat atgattttga tcataatgaa
    26641 aaagaatgcc tgatattaat gaaattttct catagaaaga gaagcggaat taacatcttg
    26701 catagcaggg caaaataaaa accgaagcca gctacaactt cggtttcttg attaaggcag
    26761 taattaagca gcggttttca gctggcttta cgctcatgtg cctgacggta tgccaccagg
    26821 tcttcaatag tcacgagcgc catattgtgt ttattggcaa actcaatgca ctctggtgca
    26881 cgcgccatcg tgccatcgtc attagtcagc tcacacagca caccagccgg tttaaagcct
    26941 gccagcgtca tcaaatcaat agtcgcttca gtatgaccgc cacgcgtcag tacaccacct
    27001 gcctgcgcgc gaagtgggaa aacgtggccc gggcgattca gatcagaagg ttttgcgcca
    27061 tcggcaatcg ctgcgcgaac ggtcgtaata cgatcagcgg cagaaacacc ggtagtcaca
    27121 ccttcagccg cttcaatggt cacggtaaaa ccggtgccat aggcgctggt gttattttct
    27181 accatcattg gcagatcaag ttgtttacgg cgatcttcag tgatgcacag gcaaacaata
    27241 ccgctaccgt ggcgaatggt cagcgccatc tgctcaacgg tcatggtttc tgccgggaag
    27301 atcatatcgc cttcgttttc acggtcttca tcatcaagca ccattacacc gcgtccttca
    27361 cgcagcgcag ccagtgcatt ttcaacacgt tcgaaaggcg taccaaaaga ggaaagtagc
    27421 gtctgattca tggtaaaaaa aacctcacta aaattatggt taccagaatc agggcagtct
    27481 taggagtggc ggcatatagc caaaataacg tgagcgggtc catgcccgac agaatcgtta
    27541 ctctctccca tccggactct aaccgtcggc cccggaatta caccggatct gctgtccttt
    27601 gagttcgcac ccaaagcgct cgcgggcttt caactgagtt gatttaccgc cggtggggaa
    27661 tttcgccccg ccctgagaat aagcggattc actataactc taatgattag cggcagcaac
    27721 gcatagcttc acataattct ggtttatgac ttacccttat cgcactacaa tggtactcaa
    27781 cacctatcag tacagggaaa ctacaatgat tgacccgaaa aaaattgagc aaatcgctcg
    27841 ccaggttcac gaatcaatgc ctaaaggaat cagggagttc ggggaagatg tggagaaaaa
    27901 aatccgccaa accctacaag ctcagctgac gcgcctcgac cttgtaagcc gtgaagagtt
    27961 cgacgtccaa acgcaagtcc tgttacggac tcgtgagaaa ctggcgctgc ttgaacaaag
    28021 aatcagcgaa ctggaaaatc gcagtactga gatcaaaaag cagccagatc cagagactct
    28081 ccccccaacg ctgtaaacat ttgtctttaa tgcactgtaa attatgaatt aatcctctaa
    28141 tttacagtgt tttctattac attatcgctt aatcccattc agagtatcat cacaaaaatg
    28201 aatgattgca attgaaaata taattgttat gtattcttta agttttactt aatcgat
//
LOCUS       DAETPZ010000048        27687 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.048, whole genome shotgun sequence.
ACCESSION   DAETPZ010000048 DAETPZ010000000
VERSION     DAETPZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.048"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..677)
                     /gene="ycjM"
                     /locus_tag="K0W59_002355"
     CDS             complement(<1..677)
                     /gene="ycjM"
                     /locus_tag="K0W59_002355"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HBI3023362.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTRPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYV"
     gene            complement(889..1203)
                     /gene="pspE"
                     /locus_tag="K0W59_002356"
     CDS             complement(889..1203)
                     /gene="pspE"
                     /locus_tag="K0W59_002356"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HBI3023363.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(1278..1499)
                     /gene="pspD"
                     /locus_tag="K0W59_002357"
     CDS             complement(1278..1499)
                     /gene="pspD"
                     /locus_tag="K0W59_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HBI3023364.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1508..1867)
                     /gene="pspC"
                     /locus_tag="K0W59_002358"
     CDS             complement(1508..1867)
                     /gene="pspC"
                     /locus_tag="K0W59_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HBI3023365.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(1867..2091)
                     /gene="pspB"
                     /locus_tag="K0W59_002359"
     CDS             complement(1867..2091)
                     /gene="pspB"
                     /locus_tag="K0W59_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HBI3023366.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2145..2813)
                     /gene="pspA"
                     /locus_tag="K0W59_002360"
     CDS             complement(2145..2813)
                     /gene="pspA"
                     /locus_tag="K0W59_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HBI3023367.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            2980..3957
                     /gene="pspF"
                     /locus_tag="K0W59_002361"
     CDS             2980..3957
                     /gene="pspF"
                     /locus_tag="K0W59_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HBI3023368.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLQDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4077..5342)
                     /gene="puuE"
                     /locus_tag="K0W59_002362"
     CDS             complement(4077..5342)
                     /gene="puuE"
                     /locus_tag="K0W59_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HBI3023369.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5380..6660)
                     /gene="puuB"
                     /locus_tag="K0W59_002363"
     CDS             complement(5380..6660)
                     /gene="puuB"
                     /locus_tag="K0W59_002363"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HBI3023370.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(6662..8149)
                     /gene="puuC"
                     /locus_tag="K0W59_002364"
     CDS             complement(6662..8149)
                     /gene="puuC"
                     /locus_tag="K0W59_002364"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HBI3023371.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8299..8856)
                     /gene="puuR"
                     /locus_tag="K0W59_002365"
     CDS             complement(8299..8856)
                     /gene="puuR"
                     /locus_tag="K0W59_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HBI3023372.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(8883..9647)
                     /gene="puuD"
                     /locus_tag="K0W59_002366"
     CDS             complement(8883..9647)
                     /gene="puuD"
                     /locus_tag="K0W59_002366"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HBI3023373.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            9859..11276
                     /gene="puuA"
                     /locus_tag="K0W59_002367"
                     /pseudo
     CDS             9859..11276
                     /gene="puuA"
                     /locus_tag="K0W59_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
     gene            11425..11589
                     /locus_tag="K0W59_002368"
     CDS             11425..11589
                     /locus_tag="K0W59_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023374.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            11579..12964
                     /gene="puuP"
                     /locus_tag="K0W59_002369"
     CDS             11579..12964
                     /gene="puuP"
                     /locus_tag="K0W59_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HBI3023375.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVHQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13098..13343
                     /locus_tag="K0W59_002370"
     CDS             13098..13343
                     /locus_tag="K0W59_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HBI3023376.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTHLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            13656..15299
                     /gene="sapA"
                     /locus_tag="K0W59_002371"
     CDS             13656..15299
                     /gene="sapA"
                     /locus_tag="K0W59_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HBI3023377.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15296..16261
                     /gene="sapB"
                     /locus_tag="K0W59_002372"
     CDS             15296..16261
                     /gene="sapB"
                     /locus_tag="K0W59_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HBI3023378.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16248..17138
                     /gene="sapC"
                     /locus_tag="K0W59_002373"
     CDS             16248..17138
                     /gene="sapC"
                     /locus_tag="K0W59_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HBI3023379.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17138..18130
                     /gene="sapD"
                     /locus_tag="K0W59_002374"
     CDS             17138..18130
                     /gene="sapD"
                     /locus_tag="K0W59_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HBI3023380.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18132..18938
                     /gene="sapF"
                     /locus_tag="K0W59_002375"
     CDS             18132..18938
                     /gene="sapF"
                     /locus_tag="K0W59_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HBI3023381.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19006..19359
                     /locus_tag="K0W59_002376"
     CDS             19006..19359
                     /locus_tag="K0W59_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HBI3023382.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            19727..20515
                     /gene="fabI"
                     /locus_tag="K0W59_002377"
     CDS             19727..20515
                     /gene="fabI"
                     /locus_tag="K0W59_002377"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBI3023383.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            20659..21786
                     /locus_tag="K0W59_002378"
     CDS             20659..21786
                     /locus_tag="K0W59_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HBI3023384.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPAIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            21854..23788
                     /locus_tag="K0W59_002379"
     CDS             21854..23788
                     /locus_tag="K0W59_002379"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HBI3023385.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(23866..23968)
                     /locus_tag="K0W59_002380"
                     /pseudo
     CDS             complement(23866..23968)
                     /locus_tag="K0W59_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24022..25996
                     /gene="pdeR"
                     /locus_tag="K0W59_002381"
                     /pseudo
     CDS             24022..25996
                     /gene="pdeR"
                     /locus_tag="K0W59_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26144..26317
                     /locus_tag="K0W59_002382"
     CDS             26144..26317
                     /locus_tag="K0W59_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023386.1"
                     /translation="MSEFDTQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26407..27156
                     /locus_tag="K0W59_002383"
     CDS             26407..27156
                     /locus_tag="K0W59_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBI3023387.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27425..27643
                     /gene="osmB"
                     /locus_tag="K0W59_002384"
     CDS             27425..27643
                     /gene="osmB"
                     /locus_tag="K0W59_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HBI3023388.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT         6438 a         7149 c         7117 g         6983 t
ORIGIN      
        1 acatactcag cacctttctc aaggtaacag agtaaaacat ccaccatcgc cagcaacact
       61 tcagggctac ggtaattcag gtcaatttga tcgtcactaa aggtggtcca taaatggcgc
      121 gtggaattat catccatttg aaacggcgtt aatagcggta acgcacgcgg acgggtgacg
      181 gcactgagat cggttttcgg atcaaccgca ataaagaaat cttcaaaacc cggaagctgt
      241 tgtaaatagt ttttaaacca ttcacttttt gcagacatat ggttgcagac aaaatcaaac
      301 attaaatgac tgcattcacc gagttgctga atatcctgcc actcccccgc ttcactggcg
      361 acctgatgat aatcaattac cgaaaatcca tcatcagatg accatgggta aaaaggtaat
      421 aaatgaacat gtgaaaaaat gctttgcagc cattgatggt agaactgatt aaatatgggt
      481 agtggtttta aatcattact gtgaaattga tcggcatagg taattaacac gacatcactt
      541 tcatcccagt gttttttacg tcgctgtgta attaatcgtt tcgcactctc aagacgcgtt
      601 acaagtttct gtaaatgagt tgcggtaaat gttccaccgt agatttcgtc caggtaatcc
      661 gtaattttct gtttcatgtt gcctttccgt ggtagcggtc ccatgaggcg agagactact
      721 ccgcagaaaa taaactgtca attgagcaca agggttttag tcacgcagga aatcattttt
      781 taccagggaa aaagcgtatt tgtgagctga cgcaaataat ctatagggat gtaaataagg
      841 tgtgacagaa aaaaaacggc gcataagcgc cgctcatggt gaattctttt aacctttgac
      901 cttcggcatt gcgatgtctt tcaggccacc ggcgttctca acgtgggtat atcccatctc
      961 gctaaggatc tcttttgcct gcccggactg gcgtccggca ttgcaataca ctttcacggt
     1021 gtcgttttta tccggaacgg cggtggcaat gcgctctttc acttccttca ggggaatatt
     1081 gatggccccc tgaacgtgct cttgctgata ctgctctgga acacgaacat cgatccagtg
     1141 ttcagcggcg aaaacgggca gtgaaaacac cagtgccaga gcaagtaagc cttttttaaa
     1201 catgtaagcg tccttctatg gatgaaaatt gccatccatt atacgcctga gatttaacaa
     1261 tttttgcgaa actcccctca ccttttataa cgctgtgcca gtttattagc agcccgactt
     1321 aacagcggtt ccagcgccac agccagcaac attttcagcg gtcggcgagc aactgatttt
     1381 atcgcccagc ccgccacacc cgccgggcca tagcgcagtg cggtaagaag taccagcttg
     1441 cctgctaatt tgaaaccaag cttcaccttt tgcccggcct gttgccagcg agtattcata
     1501 actttcctca cagttgacgg aaacggctac gtaacgtgaa agtatcggaa gtgacataac
     1561 gttccatctc gcgtaaacgc gtttcacttg ccgccagttc acggtcgact tcatccagca
     1621 attcgctgct ggaaggtagc tgctcaccaa aggccatgtt gtccggcatt ggatcaagcg
     1681 caaatgacaa aatgatgtaa gcaaccaggg taaacagcgc cagaccgaag aaaatcgaca
     1741 gcaccaccag gatacgcacc agttttaccg gtacatcaaa atagttggca atcccggcgc
     1801 agacgccgcg gaccatgccc tgctgtggaa tacgccataa ttttttattg agattaatgc
     1861 ccgccattag cgatccctcc agttcggatg ttctgcatca agaatagatt ccagcgcctg
     1921 aatacgttcg cgcatccgtt ttgcttcatc aaccagttgc gctaatcgct gctgctcact
     1981 ttgcgacaat tcactgcgac cagaacgatt gctgtaatgc agccataacc agatcggtaa
     2041 aacaaacagc acaaaaatgg ttaacggaat agccagaaat agcgcgctca taagtactcc
     2101 ttagacgatg agcggcggcg cgttcggacg ccgcctggat gttattattg attgtcttgc
     2161 ttcattttgg cttttaattg tgccagttgt tcgctgattg catcatcggc tttcagttcg
     2221 gcaaactgat cgtccagcga tttttgttta ccgaagctgt ggctttctgc ttccgcttcc
     2281 atctggtcaa tacgacgttc gaaagattcg aaacgagcca ttgcttcatc cagtttgcca
     2341 ctgtccagct gacgacgcac atcgcgcgac gagtttgccg cctgatgacg taacatcaat
     2401 gcctgctggc gagcgcgtgt ttcgctcaat ttgttttcca gctcgccaat ctctttcttc
     2461 atgcgtgcca gcgtatcgtc caccagcgtc acttcatgtt ccagggactt aatcagatcg
     2521 gtcagtttct gtttttcaat taacgctgca cgtgccagat cctctctctc tttcagcagc
     2581 gccagttcgg ctttttcctg ccattcaact tcacgcgccg acgcttgttc aatacggcga
     2641 gtcagctgtt tcttttctgc caacgcacgc gccgaagtag aacgtacttc aaccagtgta
     2701 tcttccatct cctggatcat cagacgcacc agtttctgtg gatcttccgc tttctctaac
     2761 agagcgttga tgttggcatt cacgatgtcg gcaaagcgag aaaaaatacc cataatgttg
     2821 tcctcttgat ttctgcgttc aggattgtcc tgctgaactg ttaatacaat ttgcgtgcca
     2881 attttttatc tttttgattt ataaagatct gattgaagaa tcaacagcaa catgccagga
     2941 tgaattagcg aattacacta acaagtggcg aatttcatca tggccgaata caaagataat
     3001 ttacttggtg aggcgaacag ctttctcgaa gtgctggaac aggtttcgca tctcgcaccg
     3061 ctggacaaac cggtgctcat catcggcgaa cgcggcaccg gtaaagagtt gattgccagc
     3121 cgcctgcatt atctctcctc ccgttggcaa gggccgttta tttcccttaa ctgcgcggcg
     3181 ttaaatgaaa atctgcagga ttccgaactg tttggtcacg aagcgggggc gtttaccggt
     3241 gcgcaaaaac gtcatcctgg gagatttgaa cgtgccgacg gcggtacgct atttcttgat
     3301 gaactcgcta cggcaccgat gatggtgcag gaaaaattat tgcgtgtgat tgagtacggt
     3361 gaactggagc gcgttggcgg tagccagccg ttgcaggtga atgtgcggtt ggtatgcgcg
     3421 acgaatgccg atctcccggc gatggtcaat gaaggtactt ttcgcgctga cctgctcgac
     3481 cggctggctt tcgatgtagt gcaactgccg cccctgcgcg agcgcgaaag cgacattatg
     3541 ctgatggcag aacactttgc cattcagatg tgtcgggaaa tcaagctgcc tctgttcccg
     3601 gggtttacgg agcgcgccag agaaacattg ctgaattatc gttggccggg aaatattcgt
     3661 gaattgaaaa acgtggtgga acgttcagtg tatcgccacg gcaccagcga ttatccgctt
     3721 gatgacatca ttattgatcc ctttaaacgg cgtccacctg aagaagccat cgccgtttca
     3781 gaaaatacct cgcttccgac gctgccgctg gatttacgcg agtttcagat gcagcaggaa
     3841 aaagagttgc tgcaactcag tttgcaacag gggaaatata accagaagcg cgcggctgaa
     3901 ttactggggt taacttatca tcagttccgc gcgttgttga aaaagcacca gatttagcga
     3961 agttgagcaa tacaacttgc cggatgcggc gtgaatgctt tattcggcat acataatcaa
     4021 tacgtcgttt gcaggcctga taagcgcagc gcatcagaca ttattgcgtt gggctattaa
     4081 tcgctcagcg catcctgcaa aattttcatt gccgcatcga attgcgcatc cgggatggtc
     4141 agcggataca ggaagcgaat cacgttgccg tatgcgccac aggtcagcag gagcagcccc
     4201 tgcgccagcg cgcgttgctg gattttctgt gcaatcgccg ctgacggctc gcccgtttgc
     4261 ggatcgttaa actctgccgc aatcatcgac cccaggccgc gtaccgcagc aatggccgga
     4321 acgctttctt tggcatcaat caacgtgttt ttgagacgct ggcccagttg attcgcgcgt
     4381 tcgcagagtg attctttgtc gataatattg agcaccgcgt gcgcggcagc caccgccagc
     4441 gggttcccgg cgtaggtgcc acccaacccg cccggcgcgg gcgcgtccat aatattcgcg
     4501 ttaccgacca cgcccgaaag cggcatcccg cccgcgaggc ttttcgccat cgtcattaag
     4561 tccggcttat cggcgtaatg atccatggca aacagtttac cggtacgcgc aaagccgctt
     4621 tgcacttcat cggcaatcat cacaataccg tgctcgtcgc acaggcggcg aatggcggca
     4681 accagctctt ttggcgcaac gttgaaaccg ccctcgccct gcaccggttc gaaaataatg
     4741 gccgcgacct gcttcgcttc gatgtctgat ttaaacaagc gttcgatggc gtcgagggag
     4801 tcctgtgttg aaatgccgtg taaatctgac ggataaggta cgtgatacac cgaaccaggg
     4861 aacgggccga agccgatttt gtacggcgca acttttccgg tcagcgccat ggtcatatat
     4921 gtacgaccgt ggaagccacc gctaaacgca atcacgccag gtcgtccggt atgggcgcga
     4981 gcaattttca ccgcgttttc caccgcttcc gcgccggtag tgaagaatgc ggttttagcc
     5041 tgcccgctca ctggggcaag ggcgttaatt ttctccgcca gcgtgacgta gctttcgtac
     5101 ggcacaatct gatacgccgt gtgggtaaac tgttgcaatt gctgctccac cgccgcgacc
     5161 agatcagggt ggcgatgccc ggtattcagc accgcaatgc ctgcggcgaa atcgatgtat
     5221 tcgttgccct ctacgtcctt cagcgtagcg ttttctgccg actgggcgaa gaagttacac
     5281 atcacgccaa ccccgcgtgg ggtagcagaa agacgacgct gatggaattc attgttgctc
     5341 atgtttcaca ccttttgact cttcactgtt aatcggaaat taaacgccca gacgatcgcg
     5401 caggctgtaa taagccgcgc ccatcgcggt aaatggcacg cgcagcgtgc gcccgccggg
     5461 gaacgggtaa tgcggcagat tggcgaaggc atcgaaacgc tcggcgtcac cgcgcagcag
     5521 ttcagcaatc aaacgcccgg caagatgggt acaggtgacg ccgtggccgc tgtaaccctg
     5581 catgtaatag atgttggtat caaggcggcc gaactgcggc atgcgcgaca aggtcagcag
     5641 gaagttgccc gtccagcggt aatcaatttt cacgcccttc aactgaggga aggtttttag
     5701 tagtttcggt accacaagac gctcaacgtc atccgggttg cgtgcaccat acactacacc
     5761 cccgccgtac agcaggcggt tgtcggcggt aagacggtag taatcaagca gatagttaca
     5821 gtcttccaca cagtagtttt tcgggatcag cgaacgggct aaatcttccg acagccgttc
     5881 ggtggtgatc acctgcgtgc cgcacggcat gctgcgtttc gctagttccg gctctacttt
     5941 atcgcccaga tacgcattcc cggcgacaat cacatacttc gccgtcacct gaccattggc
     6001 agtccgaacc acggctggcg tggtgtgctg gatctgcgtc acggcggaaa gctcatacac
     6061 gcgcccgccg ttcaggcgga tggcatcggc ttcaccaatg gcgaggttta gcgggtgaat
     6121 atgcccaccg ctgtgatcca gcagcgcacc agtgtagcga tcgctggcga cctcgcggcg
     6181 aatagcgttg gcgtccagca gctccagctg tttattgccg tagcgttccc agttctcttt
     6241 ctgctcttcg agtgtggcga gttgtttatc attcatcgcc acaaacagac cgccggggcg
     6301 gtagtcgcag tcaatctgat aacgtttgat acgttcgcgg atgatctcgc caccctcgaa
     6361 catcatgctg ccgagcatac gggcggtgtc catgccgtag cttttttcga tcacgtcgat
     6421 gtcgcggctg taggagttca caagctgccc gccattgcgc ccgcttgcgc caaagccgat
     6481 gcgtgaggct tcgagaacca ctacgtcaaa gcccgcttcc gccagatgca gcgcggagga
     6541 gagcccggta tagccgccgc caaccacgca aacgtcgcag gtgattgact cattcagtgt
     6601 gtcgaatggt gcatatttat tcgcactggc ggcgtagtaa ctgctggtat gttcggtcat
     6661 ttcaggcctc caggcttatc cagatggttt tcagttcagt gaatttttca agggcatgca
     6721 gggatttgtc gcgaccgttg ccgctctgct tatagccgcc aaacggcacg gtcatatcgc
     6781 cgtcgttgta gttattgacg aagacggaac cggctttcag gcgtcggctc atgcggtgcg
     6841 cgcgggagag gtcgcgcgtc cataccgccg cgccaaggcc gtactggctg tcgttggcaa
     6901 gctgcagcgc ctgatcttct gatgtgaaac gggtgaccac cagcaccgga ccgaaaatct
     6961 cttcgcggct taaggacgca ttcgggtcca catccacgaa gatggtcggg ccgatggccg
     7021 ctgccagccc ggcgttgcga ccatccagca gcagttgccc cttgccttcg ccttcacgaa
     7081 taaaggtgtg taccgagtca gcgtgagcgc tgtcgattaa ggtgcccatg gtggttgcgg
     7141 gatcaagtgg atggcccggc tgccagtttt gcgcctgctg ttttaacagg gctaagaatt
     7201 catcggcgat gctctcttcc agtaacaaac gcgttccggc gatgcacacc tggccctggt
     7261 tgtagaagat gcctgcggcg gtggcgcttg ccgctttttg cagatccgga caatcggcga
     7321 aaacgatgtt ggcgcttttg ccgcccgctt ccagccagac acgtttcatg ttgctatcac
     7381 ccgcgtcttt cagcagttgt ttacccgtac gcgtggagcc ggtaaaggcg atggcatcga
     7441 tatcgttatg acgcgaaagc gcctgccccg cttcatgacc aaaacccgtc accacgttca
     7501 acacaccatc cggcaagcct gcttctttcg ccagccccgc gagacgaatc gcactgagcg
     7561 gtgatttttc agacggtttt agaatcacgc tgtttcccgc cgccagcgcc gggccgagtt
     7621 tccagcaagt cagcaacagc gggaagttcc acggcacgat ggcggcaatc acgccgaccg
     7681 gttcacgcac gatcatcgcc agctcatggc tactggtggt cgccacttcg ccatacactt
     7741 tgtcgatcgc ttcggcgtac cagcgaatgg cgcgcgccgc gccgggaata tcatcacgca
     7801 gactgtgacg aatcggtttg ccggtgtcga gagtttccag cagtgccagc tcttcggcgt
     7861 gggcttccat taaatcggcg agtttattca gtaccgcttt acgtttagcc ggagaagaga
     7921 gtgaccagtc gccgcgttca aatacgccgc gagctgcgct caccgcacgg tcgatatcga
     7981 cgctcttgcc gcgggcaatt ttcgccagcg gtgcctgggt gaccggatca acggtttcaa
     8041 aggtttcatt ttccgccgca gcagtatatt caccgttaat aaataagcgg ttttcaatgg
     8101 cgagacttaa cgctttatcc tgccagtaag ccagatgatg aaaattcatt atgactcctg
     8161 tttcacgtct atcagatata tgcagtttgt gaacgtcagg gcgcagagat ccccgggctg
     8221 catgcccgtt tatttctggc gacattcttt atggattgcc gggcgcattg cgcttacccg
     8281 gcttacagaa caaaaagatt aaaacgtggt gggcgtatgg gcgctgataa ttcggcaaat
     8341 acctgccgac gtattactga aactgtgcgg gatgccggta ttaatggcat aactttgccc
     8401 cgcgacgagg tggtaatcct gaccattaat cgtcagaaca atttcacctt ccagtactgt
     8461 gcctatttcc tcaccctgat gcttaattct ttccccagtg gttgtgcccg gctggtacgt
     8521 ttcaaagatc atcgccagcg tgcgattcgg gttaccgtta tgaaccagct tcattgacac
     8581 accctgactg cccatctcaa ttaagtcgtc ctgattaatg acgacctgcg gctcatcagg
     8641 tttttccggc tcggaaaaga attccgagag tgacagacca tacaccttca gcagcttttg
     8701 cagcgtactg atggcagggc tgactttatc ttgttctatc gtactgatag cactgtgagt
     8761 cagcccggag agttcggcgg cacgacgttg tgaaagcccc tgctgctggc ggatttccga
     8821 caagcgtttt cctggcgcca gtccctcatc actcatattt gcatttcctt aaactgtagt
     8881 ggtcagagtc gctgtttttc agcgatatgg tgctgccaag cggtgataaa gccctcgaac
     8941 aatatacgcg aaagcgcgta ctcgctactg ttccattccg ggtgccactg tacgcccagc
     9001 gcaaaaggat gattgatgac gctaaccgcc tcaactaagc catccggcga gcgagcttca
     9061 acacgcaacc gtgggctaac gaccttcgcc ccttgtccat gtagagagtt tacccaaaag
     9121 ttgctacatt caggtaacaa cgcagacagt aatcccccct cttcaacctg aacttcatgc
     9181 gacggtgcat attgctgttc caccggcagt tcgggatctt cccgatgctc cagcaattca
     9241 ggctgttcgc acagcttgcg atgcaacgaa ccaccggttg ccaccaccag ttcttgtaaa
     9301 ccccggcaga tggcgaaaat ggggatgcgc ctttcgagtg cggcattaat tatcgccatg
     9361 ctcagaagat cacgcccggg atcggcgtca ggctcatcgc cgttttcacc atataggtgc
     9421 ggctgcacat tgctgggact actaggaaga taaatgccat cgagtttcgg caaaagttgt
     9481 tcaagtaacg acggttccgc cagcgcatgt ggtagcgcaa ttggcaagcc gcctgcgtgg
     9541 atgatggcat tcaggtactt ttcttgcaga gtctgggtcg catgaccctt aagcctgttc
     9601 ctgcacatta cgacaccgat aaccggattg ttcattatat tttccatgct caatctcaca
     9661 aagtggacta aattatcgcc attactgcgc tttaaatgac ctttatgttc aatatttttt
     9721 caatctagca gtggattttc atttttgcaa actaaattta acatttgaca aacatttggt
     9781 ttgcatacag attcgaatga tggtcattat attttacgct ttgataacga gcggaaaaca
     9841 aaccaaaggc gaagaatcat ggaaaccaat atcgttgaag tagagaactt tgttcagcag
     9901 tcagaagaga ggcggggtag cgcctttacg caggaagtga aacgctacct ggagcgctac
     9961 ccgaatacgc aatatgttga tgttttactt actgatttga atggttgctt ccgtggaaaa
    10021 cgtatccctg tttcaagcct gaagaagctc gagaaagggt gttatttccc ggcctcggta
    10081 tttgcaatgg atattctggg caacgtagta gaagaagcag gtctgggtca ggaaatgggc
    10141 gagccggatc gtacctgtgt tcctgttctc ggttccttaa ctccttctgc cgccgatcca
    10201 gagtttatcg atcagatgct cctgaccatg gtcgatgaag atggcgctcc ctttgacgtt
    10261 gagccgcgga acgttcttaa ccgcctgtgg cagcagctgc gccagcgcgg attgttcccg
    10321 gttgtagcgg tagagctgga gttctattta ctggatcgcc agcgcgacgc tgaaggggat
    10381 ctgcaaccgc cctgcgcgcc tggcaccgat gaccgcaata cacaaagcca ggtttactcc
    10441 gttgataacc tcaaccactt cgccgacgtg ctcaatgata ttgatgaact ggcgcagttg
    10501 cagctgattc cggcagatgg cgcggtcgct gaggcctcgc cgggtcagtt tgaaatcaac
    10561 ctttaccata ctgataacgt gctggaagcc tgcgatgatg cgctggcact aaaacgcctg
    10621 gtgcgtctga tggcagaaaa gcataagatg cacgccactt ttatggcgaa gccgtatgaa
    10681 gagcacgcgg gcagcgggat gcatatccat atcagtatgc aaaataatcg tggcgagaat
    10741 gtgctttctg acacagaagg cgaagattcg ccgctgctga aaaagatgct cgccgggatg
    10801 attgacctga tgccgtcgtc gatggcgttg ctggcaccaa acgtgaactc gtatcgccgc
    10861 ttccagccgg gaatgtatgt gccgacgcag gcgtcgtggg gccataacaa ccgcaccgtc
    10921 gccctgcgta ttccgtgcgg tgaccgtcat aatcaccgcg tggaatatca cgtggcgggt
    10981 gccgatgcca acccatatct ggtgatggca gcgatttttg ccggtatttt gcatggcctt
    11041 gataacgagc tgccgttgca ggaagaagtc gaaggcaacg ggctggaaca ggaaggctta
    11101 cccttcccga ttcgccagag cgatgcctgg gtgagtttat cgagaatgat cacctgcgcc
    11161 gctatttagg cgaacggttt tgccatgtgt atcacgcctg taaaaatgat gagctgttgc
    11221 agtttgagcg cctcatcaca gaaacagaaa ttgagtggat gttgaaaaac gcgtaattcc
    11281 ccttgcgggg ttgtttcatc ggcctgagcg gagccaggcc tgacatttgc tggtaatcca
    11341 gacatttccc ggatgtcgcg ttatggagcg caggcggcaa cgggcttccg ttaacgatta
    11401 agactgtgac aaggaaatga gatgatgccg atgattaaat ccccgcatgg cgagggaggc
    11461 tgcgtatgcg cccctcctgc tactgactgg actccgcccc ctttgcttcc cttgctaaac
    11521 aggtttgatt tcaggtcgac acgaccgcaa accttattac gcaggggagg cagcaattat
    11581 ggctattaat tcaccactga atattgctgc gcaacccggc aaaacccgtc tgcgaaaatc
    11641 actgaaattg tggcaggtgg taatgatggg tctggcctat ctcacgccga tgaccgtatt
    11701 tgatactttt ggtattgtct ccggcattag cgacggtcac gttccggcgt cctatttgct
    11761 ggcgctggcg ggcgtgctgt ttaccgctat cagctacggc aaactggttc accagtttcc
    11821 ggaggccggt tcggcctata cctacgcgca aaagtcgatt aacccgcacg tcggatttat
    11881 ggtcggctgg tcatcactgc tggattatct ctttttgcca atgatcaacg ttttgttggc
    11941 gaaaatctat ctctccgccc tcttcccgga agtgccgccg tgggtgtggg tggtaacctt
    12001 cgtcgccatt ttaaccgccg cgaatctgaa gagcgtcaac ctggtcgcta acttcaatac
    12061 cctgtttgta ctggtgcaaa tctccatcat ggtggtgttt atcttcctgg tggttcaggg
    12121 actgcataaa ggagaaggcg ttggcaccgt ctggtcactt cagccgttta tcagcgagaa
    12181 cgcgcacctg atcccgatta ttaccggtgc gacgattgtc tgtttctcgt tcctcggttt
    12241 cgatgcggtg accacgcttt cggaagagac gccggacgcc gcacgcgtga tcccgaaagc
    12301 catcttcctg acggcggtat acggcggcgt tatctttatc gcggcgtcgt tctttatgca
    12361 gctgttcttt cccgatatca gccgctttaa agacccggac gccgcactgc ctgaaattgc
    12421 gctgtacgtc ggcggcaagc tctttcagtc gattttcctc tgcaccacgt ttgtgaacac
    12481 attagcgtct ggcctggctt cacatgccag cgtgtcgcgt ctgctgtatg tgatggggcg
    12541 tgacaatgtg tttccggagc gcgtgtttgg ctatgtgcac ccaaaatggc ggactccggc
    12601 actcaacgtc attatggtcg ggattgtcgc gctgtcggcg ctgttctttg atttagtcac
    12661 cgcgacagca ctgattaact tcggtgctct ggtggcgttt accttcgtga atctgtcggt
    12721 gtttaatcat ttctggcggc gtaaaggaat gaataaaagc tggaaggatc acttccacta
    12781 tttgctgatg ccgctggttg gcgcgctgac ggtgggtgtg ctgtgggtta acctagagtc
    12841 aacgtcactg acactcggtc tggtatgggc ttcgttaggc ggcgcatatt tgtggtatct
    12901 gatccgccgc tatcgcaaag tgccgctgta cgaaggtgac agaacgccgg tgagcgaaac
    12961 gtaatagccg gatgcgcggc gcattatcct gcctgagggt aacgcgccgc gccattaact
    13021 ataaaaagat gtatgacccg gatcacgagt gctatcatcc gcccttatgc ttagcgcatt
    13081 aaccccaggg gcaaataatg aaccacgata tcccgctaaa atattttgat attgccgatg
    13141 agtacgcgac cgaatgcgca gagcctgtcg cagaggctga acgcacaccg ctggcccact
    13201 acttccagtt actgctcacc catttgatga acaatgaaga gatcagcgaa gaagcccaac
    13261 atgaaatggc tgctgaagcg gggattaatc ccgtacgcat tgatgagatc gcagagtttt
    13321 tgaatcaatg gggtaatgag taattattta attgcagacg atcggtcagt atcgttgtcc
    13381 ggataaagcg ttcacgccgc atccggctaa caatgacacg cactgcagcg tgctttaatg
    13441 ggtttctcac gatcgtaccg ctctgctcct cgctaagttg ttctgtgagt tatagttata
    13501 gagaaatcac gggtacgaat gttgaataga tggcgacgcc acctgttcaa cgaccaacca
    13561 cacggccctc attacgaccc ctgaacacaa acggtaatgg caaatgtcat taaatattag
    13621 taagatagcc tctttacata aaatccctta atattatgcg ccaggtatta tcgtctcttt
    13681 tggtgattgc tggacttgtg agtggtcagg caatcgccgc gcctgaatcc cccccgcatg
    13741 ctgatatccg cgacagcggt tttgtctatt gcgtcagcgg gcaagtcaac acctttaacc
    13801 catccaaagc gagcagtggg ttaattgtcg atacccttgc cgcccagttt tatgatcgac
    13861 tgctggatgt cgatccctat acctatcgcc tgatgccgga acttgccgaa agctgggaag
    13921 tactcgacaa cggcgcgacc tatcgcttcc acctgcgtcg cgatgttccg tttcaaaaaa
    13981 ccgactggtt tactcccact cgtaaaatga atgccgacga tgtggtgttt accttccagc
    14041 gaatttttga ccgcaacaac ccgtggcata acgtcaacgg cagcaacttc ccctacttcg
    14101 acagcctgca atttgccgat aacgtgaaaa gcgtccgcaa actggataat cataccgttg
    14161 agttccgact cgcccagccg gatgcttctt ttttgtggca cctcgcaacc cattatgctt
    14221 cggtcatgtc ggcagaatat gcccggaagt tagagaaaga agatcgccag gagcaactcg
    14281 accgtcaacc ggtcggcacc ggtccgtatc agttgtcgga ataccgcgcc gggcaattta
    14341 ttcgcctaca acgtcatgat gacttctggc gcggtaaacc gttaatgccg caggtagtgg
    14401 tggatttagg ctccggcggc accggacgtc tgtcgaaact cctgaccggg gaatgcgacg
    14461 ttctggcctg gcctgctgcc agccagctat ccattttgcg tgacgacccg cgcttgcgtt
    14521 taacgctgcg tcctgggatg aacgtcgcct atctggcatt taacaccgcc aaaccgccgc
    14581 taaataatcc cgctgtccgc catgcactgg cactggcgat taataaccag cgcctgatgc
    14641 aatccatcta ttatggtacg gctgaaacgg cggcctctat tttaccgcgc gcctcgtggg
    14701 cctatgacaa cgaggctaaa attactgaat acaatccggc gaaatcgcgc gaacagttga
    14761 aggcgttggg gctggaaaac ttaacgctga aactgtgggt gcccacacgt tcgcaggcgt
    14821 ggaaccccag tccactgaaa actgccgaac tgattcaggc ggatatggcg caggttggcg
    14881 taaaagtggt gattgtgccg gtagaaggtc gctttcagga ggcgcggttg atggatatga
    14941 gccatgatct gacgttatcc ggttgggcga cggacagtaa cgacccggac agtttcttcc
    15001 gtccgttact gagctgcgcg gcaattcatt cacagaccaa cctcgcccac tggtgcgatc
    15061 cgaaattcga cagcgtgttg cgtaaggcgc tctcctcgca gcagctggca gcgcgtattg
    15121 aagcctatga cgaagcgcag agtattctgg cgcaggaatt acctattctg ccgctggcgt
    15181 cgtcattgcg tttgcaggcc tatcggtacg atatcaaagg tctggtactt agcccgtttg
    15241 gtaacgcctc ctttgctggg gtgtatcgcg agaaacagga tgaggtgaaa aaaccatgat
    15301 tatcttcacc ttacgccgca ttttgttatt gattgtcacc ttgttcctgc tgaccttcgt
    15361 tggcttcagc ttgagctatt tcactccaca cgcgccgtta caaggcgcgt cactgtggaa
    15421 tgcctgggtg ttctggttta atggcctgat ccactgggat tttggtgttt ccagcattaa
    15481 cggtcaacca attgccgaac agttaaaaga ggtcttcccg gcgacgatag aactgtgcat
    15541 cctcgccttc ggttttgcgc tgattgttgg catccccgtg gggatgattg ccgggattac
    15601 gcgccataag tggcaggata acttgatcaa cgccatcgcc ttactgggct tttcaatccc
    15661 ggtgttctgg ctggcgcttc tgttgacact gttttgttca ctcacgctag gttggttgcc
    15721 cgtttcaggg cgtttcgatc tgctctacga agtgaaaccg attaccggtt ttgcgttgat
    15781 tgatgcctgg ctttcggact caccatggcg ggatgaaatg atcatgagcg caatccgcca
    15841 tatgatattg cccgtgatca ccctgtcggt tgcgccaaca actgaagtga tccgcctgat
    15901 gcgtatcagc actattgaag tgtatgacca gaactatgta aaagcggcgg cgacccgcgg
    15961 tttgtcgcgc tttactattt tgcgtcgcca cgttctgcat aacgcgttac cgccggttat
    16021 tcctcgcctg ggcttacagt tttccaccat gctgacgctg gcgatgatca ccgaaatggt
    16081 ttttagctgg ccgggtttag ggcgctggtt aattaacgcc atccgccagc aggattacgc
    16141 agccatttcc gccggagtga tggtgtgtgg ctcactggtc attattgtta acgtgatttc
    16201 tgatattttg ggtgccatgg ctaaccctct gaaacataag gaatggtatg ccttacgata
    16261 gcgtatacag cgaaaagcgc ccgccgggca cgctgcgtac cgcctggcgc aaattttata
    16321 gtgacgcctc tgcgatggtc ggcctgtacg gctgcgcggg actggctgta ctgtgtattt
    16381 ttggcggctg gtttgcgcct tacggcatcg accagcaatt tctcggttat caattattgc
    16441 cgccatcatg gtcgcgctat ggcgaagttt ctttcttcct ggggactgac gacctcggac
    16501 gtgatgtttt aagccgttta ctgagcggag ctgcgccaac cgtgggtggc gcatttgtgg
    16561 tgacgcttgc cgcgacgatc tgtggcctgg tactgggcac cttcgccggg gcgacacacg
    16621 gcctccgctc agcggtgctt aaccatattc tggatacatt actggcgatc ccttcgctgc
    16681 tgttggcaat tatcgttgta gcgtttgccg gaccgagttt gtcccacgct atgtttgctg
    16741 tctggctggc attgctgccg cgtatggtac gttcgattta cagcatggtg catgacgaac
    16801 tggaaaaaga gtacgttatc gccgcccgtc tggatggcgc atcaacgctg aatattctct
    16861 ggtttgctgt gatgccaaac atcaccgccg ggctggtcac cgagatcacc cgcgcactgt
    16921 cgatggcaat tctcgatatc gccgcgctgg gctttctcga tctcggcgca caactcccct
    16981 cgcctgaatg gggagcgatg ctcggtgatg cgctggaact gatttatgtc gccccgtgga
    17041 ctgtcatgct gccaggtgcg gcaattatga ttagcgtgtt gttggttaac ctccttggcg
    17101 atggcgttcg tcgtgcgatt attgcggggg tggaataatg ccattactcg atattcgtaa
    17161 cctgaccatt gaatttaaaa ccggtgatga gtgggttaaa gccgtcgacc gggtaagtat
    17221 gaccttaacc gaaggtgaaa tccgcggtct tgttggtgaa tccggttccg gcaagagttt
    17281 gattgcgaaa gcgatttgtg gggtgaataa agataactgg cgtgttactg ctgaccgtat
    17341 gcgttttgat gatatcgatt tgctgcgtct ctccgcacgc gaacggcgca aactggttgg
    17401 ccataacgtg tcgatgattt tccaggaacc gcagtcgtgt cttgaccctt cagaacgtgt
    17461 gggtcgccag ttgatgcaaa acatcccagc ctggacctat aaaggccgtt ggtggcagcg
    17521 ttttggctgg cgcaaacgcc gtgccattga actgctgcac cgcgtgggga ttaaagatca
    17581 caaagatgcg atgcgcagtt ttccctatga gttgaccgaa ggtgaatgtc agaaagtgat
    17641 gatagccatt gcactggcga atcaaccgcg tctgctgatt gctgacgaac cgacaaactc
    17701 aatggagcca acaacccagg cgcaaatctt tcgcctgctg acgcgtctca accaaaacag
    17761 caataccact attttgctta tcagccatga cttacaaatg cttagccaat gggcggataa
    17821 aattaacgtg ctttactgcg gtcaaacagt ggaaaccgcg ccgagtaagg agttggtgac
    17881 gatgccacat catccttata cccaggcgct gatccgcgcg ataccagact tcggcagcgc
    17941 gatgccgcat aaaagtcgcc tcaatactct acccggcgct atcccattgc tggaacagtt
    18001 accgattggt tgtcgtctgg ggccacgttg cccgtatgca caacgagaat gcattgtgac
    18061 gccacgtttg acgggggcga aaaatcatct ctatgcctgt catttcccgc tgaacatgga
    18121 gaaagagtga gatgatcgaa acgctgcttg aagtgcgtaa tttaagtaaa acgttccgct
    18181 accggaccgg ttggtttcgt cgtcagaccg tagaagcggt aaaacccttg agctttacgc
    18241 tacgtgaagg ccagacactg gcgattattg gcgagaatgg ttcgggtaaa tccacactgg
    18301 caaaaatgct ggcgggaatg atagaaccta ccagcggcga gttattgatt gacgatcatc
    18361 cgctgcattt tggcgattat tccttccgta gtcagcgtat tcgcatgatt tttcaggatc
    18421 cttcgacctc gttgaatccc cgtcaacgta tctcacaaat tctggatttt ccactgcgcc
    18481 tgaacaccga cctggaaccg gaacagcgtc gcaaacagat tattgaaacg atgcgtatgg
    18541 ttgggctgct accggatcac gtcagttact atccgcatat gttggcaccc ggacaaaaac
    18601 agcgtctggg tctggcgcgc gcgctgatat tgcgcccaaa agtcattatt gccgatgaag
    18661 ccctcgcttc actggatatg tcgatgcgtt cgcagttgat taatctgatg ctggagttac
    18721 aggaaaaaca gggcatttcg tatatttatg ttacccagca tatcggaatg atgaagcaca
    18781 ttagcgacca ggtgctggtg atgcatcagg gcgaggttgt cgagcgaggc agcaccgcgg
    18841 atgtgctggc atcgccgctg catgaactca ccaaacggct gattgccggg cactttggcg
    18901 aagcattgac agcggatgcg tggcgtaaag atcgctaatt ttctgaatcc tccttccaga
    18961 ttattcacct cattctgaca agagattaaa tcttgtcaga atgaggtgat ggataaaata
    19021 aaatcaaatg cgcgtgattt aagacgcaat ctcactttgc aggaacgaaa gctctggcga
    19081 tatcttcgca gccgacgttt tggtgatttc aaatttcgcc gtcaacatcc ggtggggagc
    19141 tacattctcg attttgcttg ctgctcggcg cgcgtagtcg ttgagctgga tggtgggcag
    19201 catgatttgg cagttgccta tgataccagg cgcactagct ggcttgagtc gcagggctgg
    19261 accgtgctgc gtttctggaa taacgagatt gattgtaatg aggaggcggt gctggagatt
    19321 attctgcagg aactgaaccg ccggtcaccc tcaccctgaa agggcgaggg ggccgaccga
    19381 gccgaatagc tgttgtggtg aaaacatgga gacagtgctg gagaatattc ggcaaggtct
    19441 gaaccatccc agccatcgcc ataaaagggt taggggctgt atgagcctgt ttgttgctgg
    19501 ggtaacaata tttgcacaat atggtcccct cgcccctctg gggagagggt tagggtaagg
    19561 ggaaaagcgc cccccctgcc gcagcctgct ccggtcggac ctggcaacta tagctactca
    19621 cagccaggtt gattataata accgtttatc tgttcgtact gtttactaaa acgacgaatc
    19681 gcctgatttt caggcacaac aagcatcaac aataaggatt aaagctatgg gttttctttc
    19741 cggtaagcgc attctggtaa ccggtgttgc cagcaaactg tccatcgcct acggtatcgc
    19801 tcaggcgatg caccgcgaag gagctgaact ggcattcacc taccagaacg acaaactgaa
    19861 aggccgcgta gaagaatttg ccgctcaatt gggttctgac atcgttctgc agtgcgatgt
    19921 tgcagaagat gccagcatcg acaccatgtt cgctgaactg gggaaagttt ggccgaaatt
    19981 tgacggtttc gtacactcta ttggttttgc acctggcgat cagctggatg gtgactatgt
    20041 taacgccgtt acccgtgaag gcttcaaaat tgcccacgac atcagctcct acagcttcgt
    20101 tgcaatggca aaagcttgcc gctccatgct gaatccgggt tctgccctgc tgaccctttc
    20161 ctaccttggc gctgagcgcg ctatcccgaa ctacaacgtt atgggtctgg caaaagcgtc
    20221 tctggaagcg aacgtgcgct atatggcgaa cgcgatgggt ccggaaggtg tgcgtgttaa
    20281 cgccatctct gctggtccga tccgtactct ggcggcctcc ggtatcaaag acttccgcaa
    20341 aatgctggct cattgcgaag ccgttacccc gattcgccgt accgttacta ttgaagatgt
    20401 gggtaactct gcggcattcc tgtgctccga tctctctgcc ggtatctccg gtgaagtagt
    20461 ccacgttgac ggcggtttca gcatcgctgc aatgaacgaa ctcgaactga aataatcgtt
    20521 ctgttggtaa agatgggcgg cgttctgccg cccgttatct ctgttatacc tttctgatat
    20581 ttgttatcgc cgatccgtct ttctcccctt cccgccttgc gtcaggataa cgatttcctt
    20641 tacgaccaag gagcgcccat ggaacaacgc cacatcaccg gcaaaagcca ctggtatcat
    20701 gaaacgcaat ccagtactac ggagtatgac gttctgcctc tggtcccgga agccgcaaag
    20761 gtcagcgatc cctttctgct cgacgtgatc cttgatgaag agacgctggc ccccttcctt
    20821 tcatggctgg tccctgcgcg cgttcttgca gtggagttgt tccctgacca gcttaccgtg
    20881 acccgttcac aaaccttcac cgcttatgaa cgcttgtcga cggccctgac ggttgctcag
    20941 gtttgcggcg tccagcggtt atgtaactac tattcggcgc gacttacgcc gctccccgga
    21001 cctgattcct ccagggaaag taatcatcgg ttggcacaaa tcacgcaata tgcccgccaa
    21061 ctggctagct cgcctgctat tatcgacaac cgatcgcgcc agcatctgaa tgacgtcggt
    21121 cttactgtct gggactgtgt gatcattaac caaatcattg gttttattgg ctttcaggcg
    21181 cggacaattg cgacatttca ggcttatctc gggcatccgg tacgctggtt acccgggctg
    21241 gcgatacaaa actacgccga cgcgtcactg tttgctgatg aatcaatacg ctggcgaagc
    21301 agctatgagg tggaaaaact acctgaagag cacacaaaaa gttcaactgc agaactttgc
    21361 caactggccg aaacacactc tctccaccct atttcacttt cccttctcga aaagttgtta
    21421 aacagcacac gggtcaatac acagccggat aatcagcttg cggcgttgtt atgcgcgcgt
    21481 ataaatggca gtcctgcttg tttttccacc tgtatggatt catcaaatga atataaaaaa
    21541 atcagcaccc ttctgcgcaa gggcgaaaat gaaattaacc gatgggctga ccgtcattct
    21601 gttgagcgcg ctaccgttca ggcgatacaa tggctgaccc gagcacccga tcgctttagc
    21661 gccgcccagt tcagcccttt actcgaacac gaaaaatcat caacgcagat tattaatctg
    21721 ctggtatgga gcgggctgtg tggctggata aatcgcttaa aaatcgcgtt gggtgagaca
    21781 tattaacctt gccgcgtcag acagattcgc gtaaaactgt cagccgctct aatggccacc
    21841 aaaatagaca attatgtttc aggacaaccc gctgctagcg cagcttaaac agcaactgca
    21901 ttcccagacg ccacgcgctg aaggggtggt aaaagccaca gaaaaaggct ttggcttcct
    21961 ggaagtcgac gcacaaaaaa gttatttcat tccgccgccg cagatgaaaa aagtcatgca
    22021 tggcgaccga attatcgcgg tgatccacag tgaaaaagaa cgtgaatccg cagagccaga
    22081 agaactggtt gaaccgttcc tgactcgttt cgtgggtaaa gttcagggca aaaatgaccg
    22141 tctgaccatc gttcctgatc atccactttt aaaagacgcc attccttgcc gcgcagcccg
    22201 tggcctgaac cacgagttta aagaaggcga ctgggcggtt gccgaaatgc gccgtcatcc
    22261 gctgaaaggc gatcgttctt tctatgctga actgacacaa tacatcactt ttggcgacga
    22321 ccactttgta ccgtggtggg ttacccttgc gcgccataat ctggaaaaag aagcaccaga
    22381 cggcgtcgct accgaaatgc tcgatgaagg tctggttcgt gaagatctga cctcgctgga
    22441 ttttgtcacc atcgacagtg ccagcactga agatatggat gacgcccttt tcgctaaggc
    22501 gctgccggat gacaaacttc agctgattgt ggcgattgcc gatccaaccg cgtggattgc
    22561 tgaaggcagc aagctggaca aagccgcgaa aattcgcgca ttcaccaact atctgcctgg
    22621 cttcaacatc cctatgctgc ctcgcgagct ttctgacgat ctctgctcac tgcgcgccaa
    22681 tgaagtccgc ccggtactgg catgccgcat gacgctctcc gctgatggca ccattgaaga
    22741 taatatcgaa ttctttgccg ccaccatcga atccaaagcg aagctggtgt atgaccaggt
    22801 ttctgactgg ctggaaaata ccggtgactg gaagcctgaa agtgaagcaa ttgccgaaca
    22861 agtccgtttg ctagcgcaaa tttgccaacg ccgcggcgag tggcgtcata accacgcact
    22921 agtgtttaaa gatcgcccgg attaccgctt tattctcggt gaaaaaggtg aagtgctgga
    22981 tatcgtcgcc gagcctcgtc gcattgccaa ccgtatcgtc gaagaagcga tgattgccgc
    23041 taacatttgt gcggcccgcg tactgcgcga taagctcggt tttggcatct ataacgtgca
    23101 tatgggcttt gatccggcga atgccgacgc gctggcagcg ttgctgaaaa cgcacggtct
    23161 gcatgtcgat gccgaagaag tactcacgct ggacggtttc tgcaaactgc gtcgtgaact
    23221 ggatgcgcaa ccaactggtt tcctcgacag ccgcattcgt cgcttccagt catttgctga
    23281 aattagcact gaacccggtc ctcactttgg cctcggtctg gaagcatacg ccacctggac
    23341 ttcgccgatc cgtaaatatg gcgacatgat caaccaccgt ctgctgaaag cggttatcaa
    23401 aggcgaaact gcgacgcgtc cgcaggatga aatcactgtc caaatggccg aacgtcgccg
    23461 tctcaaccgg atggcagaac gtgatgttgg cgactggtta tacgcacgct tcctgaaaga
    23521 taaagccggg accggcaccc gtttcgcggc ggaaattgtc gatatcagcc gtggcggcat
    23581 gcgtgtgcgt ttggttgata acggcgctat cgcctttatt ccggcacctt tcttacacgc
    23641 tgtgcgcgat gaactggttt gcagccagga aaacggcact gtacaaatta aaggtgaaac
    23701 ggtttacaaa gtaactgacg ttattgacgt caccattgcc gaagtccgca tggaaacccg
    23761 cagcattatt gcgcgcccgg tcgcgtaatc tcctttcacg gcccattcct catggatggg
    23821 ccgtttattt ccccgctcta ccttcgttat atccaggcag attttttaac tgactttcgt
    23881 ttgaaaactg gcgtttttcc aaaactggat tacaaataaa tatataaaaa acaatgcgtt
    23941 tatccgccgt taatttcatg taagtcatgt tgttcctttt atttaaccgc ggactccgct
    24001 gttaaccgga ggatatgcat catgaaaacc gttagggagt ccacaacgtt gtacaacttt
    24061 ctcggatcgc acaatccata ctggcggttg acggaaagca gcgatgcttt gcgcttttct
    24121 accaccaaaa ccacagaacc tgatcgtaca ttgcagttat ctgccgaaca ggctgctcgc
    24181 atcagggaaa tgacggtcat cacctccagc ctgatgatga gtctgaccgt cgatgaaagc
    24241 gatctttctg agcatctggt aggacgaaaa atcaataaac gggaatgggc tggcaacgcg
    24301 tctgcatggc atgacacacc ggcggttgct cgtgatttat cacacgggct ttcctttgct
    24361 gagcaggtag tttctgaagc acattccgca atagtgattc tcgacagccg ggggaatatc
    24421 caacgcttca atcggttatg tgaagattac acagggttga aagaacacga cgtcattggg
    24481 caaagcgtgt ttaaactgtt tatgagccgt cgtgaagctg cggcatccag gcgcaataac
    24541 cgtgtatttt ttcgaagcgg caatgcatat gaagtcgaac tgtggatacc aacacgtaaa
    24601 ggccagcggc tgtttctgtt tcgcaataaa tttgtccaca gcggcagtgg caaaaacgag
    24661 atttttttaa tctgttccgg taccgacatt accgaagagc gccgcactca ggagcgactg
    24721 cgtattctgg caaataccga cagtatcacc ggactgccga atcgtaacgc aatgcaggag
    24781 ttaatcgatc acgctattaa tcaggcagat aacaataaag ttggggttgt gtatcttgat
    24841 ttggataatt ttaaaaaggt caacgacgcc tatgggcatt tgtttggtga ccagttatta
    24901 cgcgacgtgt cattggctat tttaagctgt ctcgaacatg accaggtgtt ggcgcgtcca
    24961 ggtggcgatg agtttctggt actggcatcc aatacctcac aaagcgcgct ggaagcaatg
    25021 gcatcacgaa ttttaacccg cttacgcctc ccctttcgta ttggtttaat tgaagtttat
    25081 accagctgtt cagtaggtat ttcgctctct cccgaacatg gttcggacag cgcgactatt
    25141 attcgtcacg ccgacacagc aatgtacaca gcgaaggaag gcggacgagg acaattttgc
    25201 gtttttactc cagaaatgaa tcagcgggta tttgaatatc tctggctgga taccaacttg
    25261 cgtaaagcac tggaaaacga tcagttggtt atcgaaaatc acctagcgtg gcgaagtgcg
    25321 cagtctggaa gcactagtac gttggcagtc acctgaacgt gggttgattc caccgttgga
    25381 cttcatttcc tacgccgaag agtcagggct aattgtgcct ttaggccgtt gggtgattct
    25441 cgatgtcgta cgccaggtgg caaagtggcg ggataaaggc ataaacctgc gagtggcggt
    25501 aaatatttct gcacgtcagc tcgccgatca aaccattttc accgccctga aacaggttct
    25561 ccaggaactc aattttgaat actgccctat agatgttgaa ctgacagaga gttgtctgat
    25621 tgagaatgat gaactggcac tgtctgttat tcaacaattt agccaactag gtgcgcaagt
    25681 gcatctggat gattttggta ccggctactc ttcactttcg caactggcgc gctttccgat
    25741 cgatgccatc aaacttgacc aggtttttgt tcgagatatt cacaaacaac ctgtctcgca
    25801 gtcactggtc cgggcgatcg tcgctgtggc ccaggcattg aatcttcagg tgatcgccga
    25861 aggtgtagag agtgcaaagg aagatgcttt tttaaccaag aacgggatca atgagcggca
    25921 aggatttttg tttgccaaac cgatgcccgc cgtcgccttc gaacgctggt ataaacgcta
    25981 tctgaagcgc gcataagtag atgaatatat atacttagcg ctatatcccg tcattatata
    26041 tcgtattcct ggatgataat tagccgttgc tgttaaacac ttctctttta gcggcaattt
    26101 ttgcattatt cattttcccc cttttgctat aaggatgtcc ttgatgtctg aatttgatac
    26161 ccagcgcgtt gccgaacgta ttgatattgt gttagatatc cttgtggcag gcgattacca
    26221 ctctgcgatc cataatctcg agatacttaa agcagaactc ttgcgccagg ttgctgaatc
    26281 tacgccagac atcccgaaga caccgtggga gatttaacaa tcgtgccttc aggttttcct
    26341 atttgaggat gcccggcgcg tcagtatttt tttatttagt attataacgt tataagaatt
    26401 acagcgatga attcccgaca acaaactatt ctacagatgg tcattgacca gggtcaggtt
    26461 agcgtaaccg atctggcaaa agccactgga gtttctgaag ttaccattcg ccaggatctc
    26521 aacaccctcg aaaaactgag ttacctccgc cgtgcacatg gctttgcagt ttcgcttgat
    26581 agtgatgacg tcgaaacccg tatgatgagc aactatacgc tgaagcgtga actcgccgag
    26641 tttgccgcgt cactggttca accgggcgaa accatcttta tcgaaaatgg cagcagcaat
    26701 gccctgctcg cccggactct gggggagcag aagaaaaatg tcactatcat cacggtcagc
    26761 agctacatcg cgcatttgct gaaagacgcg ccttgtgaag tcattttgct cggtggcgtg
    26821 taccagaaaa aaagcgaaag tatggttggc cctttgacac gccagtgcat ccagcaggta
    26881 catttcagca aagcatttat tggtattgat ggctggcaac ctgaaactgg attcaccggt
    26941 cgcgatatga tgcgcaccga tgtggtcaat gccgtgctgg aaaaagagtg cgaagcgata
    27001 gtcctgactg acagctcgaa atttggtgct gtacattcat actccatcgg tcccgttgag
    27061 cgattcaatc gcgtgattac cgattcgaaa atacgcgcca gcgatctgat gcatcttgag
    27121 cacagtaaac tcaccgttca cgtcgttgac atttaaccat ttcccttctc tacggatgat
    27181 ttgcagtttg gcaaatcatc cgctctaaga tgattcctgg ttgataatta agactattta
    27241 cctgttatta acactctcaa gatataaaat tattatcagc gatataacag gaagtaatta
    27301 tcacgtgcgt gatataaccc tgcgcgcgag cagatttcac ggaataattt caccaggctt
    27361 attcttagct attatagtta tagagagctt acttccgtga atcataaatt caggagagag
    27421 tattatgttt gtaacgagca aaaaaatgac cgcggctgtt ctggcaatta ctttggcaat
    27481 gtctctgagt gcctgttcta actggtctaa acgggaccgc aacaccgcaa tcggcgcggg
    27541 tgcaggggca ttaggcggtg cagtactgac cgatggcagt acgttgggta cattaggtgg
    27601 tgcagctgtc ggtggtgtta ttggtcacca ggtcggtaaa taatcgtaaa taaatgtcga
    27661 ttatcgactc gcaattataa tacgctt
//
LOCUS       DAETPZ010000049        27536 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.049, whole genome shotgun sequence.
ACCESSION   DAETPZ010000049 DAETPZ010000000
VERSION     DAETPZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.049"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="K0W59_002385"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="K0W59_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HBI3023389.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="K0W59_002386"
     CDS             complement(1614..1937)
                     /locus_tag="K0W59_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HBI3023390.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="K0W59_002387"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="K0W59_002387"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HBI3023391.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="K0W59_002388"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="K0W59_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HBI3023392.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDIYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="K0W59_002389"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="K0W59_002389"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HBI3023393.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGVAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="K0W59_002390"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="K0W59_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HBI3023394.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="K0W59_002391"
     CDS             7537..8574
                     /locus_tag="K0W59_002391"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBI3023395.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="K0W59_002392"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="K0W59_002392"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBI3023396.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="K0W59_002393"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="K0W59_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HBI3023397.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="K0W59_002394"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="K0W59_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HBI3023398.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="K0W59_002395"
     CDS             10745..11626
                     /locus_tag="K0W59_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3023399.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="K0W59_002396"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="K0W59_002396"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HBI3023400.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="K0W59_002397"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="K0W59_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HBI3023401.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="K0W59_002398"
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="K0W59_002398"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBI3023402.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLISGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="K0W59_002399"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="K0W59_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HBI3023403.1"
                     /translation="MLRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="K0W59_002400"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="K0W59_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HBI3023404.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIEFLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="K0W59_002401"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="K0W59_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HBI3023405.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="K0W59_002402"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="K0W59_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HBI3023406.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHACLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="K0W59_002403"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="K0W59_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HBI3023407.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="K0W59_002404"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="K0W59_002404"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBI3023408.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="K0W59_002405"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="K0W59_002405"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBI3023409.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="K0W59_002406"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="K0W59_002406"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBI3023410.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="K0W59_002407"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="K0W59_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBI3023411.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDIQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="K0W59_002408"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="K0W59_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBI3023412.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="K0W59_002409"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="K0W59_002409"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HBI3023413.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="K0W59_002410"
     CDS             24893..25273
                     /locus_tag="K0W59_002410"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBI3023414.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="K0W59_002411"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="K0W59_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HBI3023415.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="K0W59_002412"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="K0W59_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HBI3023416.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..>27536
                     /locus_tag="K0W59_002413"
     CDS             26442..>27536
                     /locus_tag="K0W59_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HBI3023417.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSFRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVYEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQPRDPMQPFSKRSLDKLVKTYSPDGAV"
BASE COUNT         6791 a         6856 c         7220 g         6669 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tatttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccgctcc agctatcgtt agccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaagcgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa atatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagcgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc caggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgcca ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag gcagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttggtacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggcacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcact ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaattg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgacgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgatac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagagc ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca tcatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccaccg caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccattgc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggcgatcgca
    15241 aataccagcc ccggaaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatgc tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagttcc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcatgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagt atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggtta tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatac aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgcc actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatga caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc actggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac gactatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cattaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttcacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgctatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgacct caaagccaaa gtagcccggc agaagctgag cccggacagt ttcacaacct
    26881 acggggtgcg tatcgaccgg attaaagcaa cgttcggcga gcgtgaggtg ttcagcgacg
    26941 taacatacaa tcggctggtg gaagtgctgg acgagtggat cgccactcgc tcgaacaata
    27001 acgccctgga gttgtttgcc gagctccgtc ggttctggaa gttctgcgca cctactcttt
    27061 gcaacggccg caatgttgcc gccagtctgc cagatgatta tgtttccttc cgcgtacaga
    27121 aacctacccc cacacggctt tttaccgata ttgaatcaat cgcccgactc tggctcaatg
    27181 ttgctgcctg cacctctgta caccagaaga atgctgttcg cttcatgatc atcactggtg
    27241 ttcgtccgat taatgtccat aacctgcgct gggactacgt ttacgaggag gctggtgaaa
    27301 ttgtttatcc ggaaggggtt atcggcatgc gaggggctat gaaaacacaa aaggctttcc
    27361 gcctgccgat aacgcctgag atccggcgga ttatcgacga gcagaaagcc tggcgtgatt
    27421 cagttcctga gtgcaacagg gattatgtat ttttgcagcc acgtgatcca atgcagccat
    27481 tttcaaaacg atcactggat aagctggtga aaacatacag cccggacggg gctgta
//
LOCUS       DAETPZ010000050        26933 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.050, whole genome shotgun sequence.
ACCESSION   DAETPZ010000050 DAETPZ010000000
VERSION     DAETPZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.050"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..437
                     /locus_tag="K0W59_002414"
     CDS             <1..437
                     /locus_tag="K0W59_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HBI3023418.1"
                     /translation="IIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAPAPQV
                     ELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVETLAWF
                     TVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(499..1119)
                     /gene="rhtB"
                     /locus_tag="K0W59_002415"
     CDS             complement(499..1119)
                     /gene="rhtB"
                     /locus_tag="K0W59_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HBI3023419.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            1230..2252
                     /gene="pldB"
                     /locus_tag="K0W59_002416"
     CDS             1230..2252
                     /gene="pldB"
                     /locus_tag="K0W59_002416"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HBI3023420.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWHQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            2260..3060
                     /gene="yigL"
                     /locus_tag="K0W59_002417"
     CDS             2260..3060
                     /gene="yigL"
                     /locus_tag="K0W59_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HBI3023421.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            3136..4035
                     /gene="bioP"
                     /locus_tag="K0W59_002418"
     CDS             3136..4035
                     /gene="bioP"
                     /locus_tag="K0W59_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HBI3023422.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(3923..4876)
                     /gene="metR"
                     /locus_tag="K0W59_002419"
     CDS             complement(3923..4876)
                     /gene="metR"
                     /locus_tag="K0W59_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HBI3023423.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            5112..7373
                     /gene="metE"
                     /locus_tag="K0W59_002420"
     CDS             5112..7373
                     /gene="metE"
                     /locus_tag="K0W59_002420"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBI3023424.1"
                     /translation="MTILNHTLGFPRVGLRRELKKVQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(7412..8227)
                     /locus_tag="K0W59_002421"
     CDS             complement(7412..8227)
                     /locus_tag="K0W59_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBI3023425.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            8489..9250
                     /gene="udp"
                     /locus_tag="K0W59_002422"
     CDS             8489..9250
                     /gene="udp"
                     /locus_tag="K0W59_002422"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HBI3023426.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            9391..10818
                     /gene="rmuC"
                     /locus_tag="K0W59_002423"
     CDS             9391..10818
                     /gene="rmuC"
                     /locus_tag="K0W59_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HBI3023427.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPGQPNDEAYQRDDEYNQQSR"
     gene            10913..11668
                     /gene="ubiE"
                     /locus_tag="K0W59_002424"
     CDS             10913..11668
                     /gene="ubiE"
                     /locus_tag="K0W59_002424"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HBI3023428.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            11682..12287
                     /gene="ubiJ"
                     /locus_tag="K0W59_002425"
     CDS             11682..12287
                     /gene="ubiJ"
                     /locus_tag="K0W59_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HBI3023429.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            12284..13924
                     /gene="ubiB"
                     /locus_tag="K0W59_002426"
     CDS             12284..13924
                     /gene="ubiB"
                     /locus_tag="K0W59_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HBI3023430.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHRVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            14003..14272
                     /gene="tatA"
                     /locus_tag="K0W59_002427"
     CDS             14003..14272
                     /gene="tatA"
                     /locus_tag="K0W59_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HBI3023431.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            14276..14791
                     /gene="tatB"
                     /locus_tag="K0W59_002428"
     CDS             14276..14791
                     /gene="tatB"
                     /locus_tag="K0W59_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HBI3023432.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            14794..15570
                     /gene="tatC"
                     /locus_tag="K0W59_002429"
     CDS             14794..15570
                     /gene="tatC"
                     /locus_tag="K0W59_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HBI3023433.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            15612..16394
                     /gene="tatD"
                     /locus_tag="K0W59_002430"
     CDS             15612..16394
                     /gene="tatD"
                     /locus_tag="K0W59_002430"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HBI3023434.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(16391..16879)
                     /gene="rfaH"
                     /locus_tag="K0W59_002431"
     CDS             complement(16391..16879)
                     /gene="rfaH"
                     /locus_tag="K0W59_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HBI3023435.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            17046..18539
                     /gene="ubiD"
                     /locus_tag="K0W59_002432"
     CDS             17046..18539
                     /gene="ubiD"
                     /locus_tag="K0W59_002432"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HBI3023436.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVVTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVTYIDAIWDELAIFNNGKSA"
     gene            18585..19286
                     /gene="fre"
                     /locus_tag="K0W59_002433"
     CDS             18585..19286
                     /gene="fre"
                     /locus_tag="K0W59_002433"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HBI3023437.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(19379..20542)
                     /gene="fadA"
                     /locus_tag="K0W59_002434"
     CDS             complement(19379..20542)
                     /gene="fadA"
                     /locus_tag="K0W59_002434"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HBI3023438.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARIHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(20552..22741)
                     /gene="fadB"
                     /locus_tag="K0W59_002435"
     CDS             complement(20552..22741)
                     /gene="fadB"
                     /locus_tag="K0W59_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HBI3023439.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEITAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            22931..24262
                     /gene="pepQ"
                     /locus_tag="K0W59_002436"
     CDS             22931..24262
                     /gene="pepQ"
                     /locus_tag="K0W59_002436"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HBI3023440.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVITLPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLGLIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            24262..24876
                     /locus_tag="K0W59_002437"
     CDS             24262..24876
                     /locus_tag="K0W59_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HBI3023441.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            24915..26366
                     /gene="trkH"
                     /locus_tag="K0W59_002438"
     CDS             24915..26366
                     /gene="trkH"
                     /locus_tag="K0W59_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HBI3023442.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPAINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            26378..26923
                     /gene="hemG"
                     /locus_tag="K0W59_002439"
     CDS             26378..26923
                     /gene="hemG"
                     /locus_tag="K0W59_002439"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HBI3023443.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         6166 a         6858 c         7431 g         6478 t
ORIGIN      
        1 tgattatcga aaaaatggcc tggctgcata cgctgattat ggtgggcggt ggcctgtatc
       61 tctgctggat gggttaccag atgctacgcg gtgcactgaa aaaagaggct gtatctgcgc
      121 ctgcaccaca ggttgagctg gcgaaaagcg ggcgcagttt cctgaaaggc ttactgacca
      181 atctcgccaa tccgaaagcg attatctatt ttggttcggt attctcattg tttgtcggcg
      241 ataacgttgg cactaccgag cgctggggca ttttcgcgtt gattattgtc gaaacactgg
      301 cgtggtttac cgtcgttgcc agtctgtttg ccctgccgca aatgcgccgt ggttatcaac
      361 gtctggcgaa gtggattgat ggttttgccg gggcgttatt tgccggattt ggcattcatt
      421 tgattatttc gcggtgatgc cagacgcgtc ttcagagtaa gtcggataag gcgtttacgc
      481 cgcatccgac attatttttc acgcatgcct cgccgatgct aacagcgctc ccaccagcat
      541 aaacaacgag ccgaaaatct tattcagcgc cttcatctgc tttggtcctt taatccatag
      601 agcaatcctt tgagcaaggg tggcgtaacc gatcatcaca ataatatcga ccacaatagt
      661 ggtgacgccg agcacgatat actgcatcag ttgcggctgt tgcggcatga tgaattgcgg
      721 aaatagcgcc gccagaaaca caatactttt gggattggtg agattcacaa aaactgcgcg
      781 ctggaacaaa tgtcgacgcg attgagtaga ggccagcgat ttaagatcaa ttgcgccagc
      841 ggcgcgccac tgctggattc ccagccaaat caagtaagcc gcgcctgccc actttaacac
      901 ttcaaacgca atcactgagc gggaaaatag cgtccccaac cccacgccaa ccagcacaat
      961 atgaatcgcc agtccggtct gaagcccagc aatagacgcc accgcgccgc gataaccgtg
     1021 gttgagcgag gtggtcatag tgttgattgc accagagcct ggcgacaggc ttaaaatgat
     1081 cgatgtcagc aggtaggcaa accaccattc taaggtcatg atgaactccc ggtgtgtcta
     1141 tttttgtgcc acaatacgct actgtcgcag cgttgtgtca ggcacgctta aaaaaacgat
     1201 tttacgtggt ttaagaggca gattacccga tgtttcagca gcaaaaagac tgggaaacaa
     1261 gagaaaacgc gtttgctgct tttaccatgg gaccgctgac tgatttctgg cgtcagcgtg
     1321 atgaagcaga gtttactggt gtggatgaca ttccggtgcg ctttgtccgt tttcgcgcac
     1381 agcaccatga ccgggtggta gtcatctgcc cggggcgtat tgagagctac gtaaaatatg
     1441 cggaactggc ctacgacctg ttccatttgg ggtttgatgt cttaatcatc gaccatcgcg
     1501 ggcagggacg ttccggtcgc ctgttagccg atccgcatct cgggcatgtt aatcgcttta
     1561 atgattatgt tgatgatctg gcggcattct ggcatcagga ggttcagccc ggtccgtggc
     1621 gtaaacgcta tatactggca cattcgatgg gcggtgcgat ctccacatta tttctgcaac
     1681 gccatccagg tgtatgtgac gccattgcgc taactgcgcc aatgtttggg atcgtgattc
     1741 gtatgccgtc atttatggca cggcagatcc tcaactgggc cgaagcgcat ccacgtttcc
     1801 gtgatggcta tgcaataggc accgggcgct ggcgcgcgtt gccgtttgct atcaacgtac
     1861 tgacccacag cagacagcga tatcgacgta acttacgctt ctatgctgat gacccaacga
     1921 ttcgcgtcgg tgggccgacc taccattggg tacgcgaaag tattctggct ggcgaacagg
     1981 tgttagccgg tgcgggtgat gacgccacgc caacgcttct cttacaagct gaagaggaac
     2041 gcgtggtgga taaccgcatg cacgaccgtt tttgtgaact ccgcaccgcc gcgggccatc
     2101 ctgtcgaagg aggacggccg ttggtaataa aaggtgctta ccatgagatc ctttttgaaa
     2161 aggacgcaat gcgctcagtc gcgctccacg ccatcgttga ttttttcaac aggcataact
     2221 cacccagcgg aaaccgctct acagaggttt aaatttctta tgtaccaggt tgttgcgtct
     2281 gatttagatg gcacgttact ttctcccgac catacgttat ccccttacgc caaagaaact
     2341 ctgaagctgc tcaccgcgcg cggcatcaac tttgtgtttg cgaccggtcg tcaccacgtt
     2401 gatgtggggc aaattcgcga taatctggag attaagtctt acatgattac ctccaatggt
     2461 gcgcgcgttc acgatctgga tggcaatctg atttttgctc ataacctgga tcgcgacatc
     2521 gccagcgatc tgtttggcgt agtcaacgac aatccggaca tcattactaa cgtttatcgc
     2581 gacgacgaat ggtttatgaa tcgccatcgc ccggaagaga tgcgcttttt taaagaagcg
     2641 gtgttcaaat atgcgctgta tgagccggga ttactggagc cggaaggcgt cagcaaagtg
     2701 tttttcacct gcgattccca tgaacaactg ctgccgctgg agcaggcgat taacgctcgc
     2761 tggggcgatc gcgtcaacgt cagtttctct accttaacct gtctggaagt gatggcgggc
     2821 ggcgtgtcga aaggccatgc gctggaagca gtggcgaaga aactgggcta cagcctgaag
     2881 gattgtattg cgtttggtga cgggatgaac gacgccgaaa tgctgtcgat ggcggggaaa
     2941 ggctgcatta tgggcagtgc gcaccagcgt ctgaaagatc tgcatccgga gctggaagtt
     3001 attggtacta atgccgacga cgcggtgccg cattatctgc gtaaactcta tttatcgtaa
     3061 tcgttcttta tttggtcagt tgtcaacctg atacttcgct acaatggata ctcgttaatc
     3121 aaagagtttt ccattgtggc gctacttatc atcaccacga ttctgtgggc cttctccttt
     3181 agcttttatg gcgagtacct tgcggggcac gtcgatagct attttgcggt gctggtgcgc
     3241 gttggcttgg cggcactcgt ttttctgccg tttctgcgta cccgtagcaa tagcctgaaa
     3301 acggtcggcc tgtatatgct ggtgggcgcg atgcagctcg gcgtgatgta tatgctgagt
     3361 ttccgcgctt atctctacct gacggtctcc gaattgttgc tgttcaccgt actgacgccg
     3421 ctctacatca cgctgattta tgacatcatg agtaagcgcc gtctgcgctg gggctatgct
     3481 tttagcgcct tgctggcggt gattggtgcc gggattattc gctatgatca ggtcaccgac
     3541 catttctgga ctggcttgtt gctggtgcaa ctctccaata tcacttttgc catcggcatg
     3601 gtgggttaca aacgcctgat ggaaactcgc ccgatgccgc agcataacgc ctttgcgtgg
     3661 ttctatcttg gcgcgttcct ggtggcggta attgcatggt tcttgctggg aaatgcgcag
     3721 aaaatgccgc aaaccacgtt gcaatggggc attctggtgt ttcttggcgt ggtggcttcc
     3781 gggattggct actttatgtg gaactacggc gcgacgcagg tggacgccgg aacgctgggc
     3841 attatgaata atatgcacgt tccggcaggg ctgctggtaa acctggctat ctggcaccaa
     3901 cagccgcact ggccaacgtt tattacaggc gcgctggtga tcctggcctc actgtgggtg
     3961 catcgtaagt gggtcgctcc gcgctcttca caaacggcag atgatcgcag gcgtgattgc
     4021 gcgctgagcg aataaacgct tccgtaactg gctgacgctg ctcgccatcg cgcacggcgg
     4081 cgtacagtcg gctccacaag ccttcgccca gggttttggt caccaccaga ccctggcgct
     4141 caaaactctc tactacccaa tgcggtagcg cggcaatacc catccgcgcg gcaaccattt
     4201 gaatcaataa taaggtgtta tcgacgcttt tcagtgacgg gctgacgcct gccggctgaa
     4261 gaaaatgccg ccagacatcc agtcgactac gctgcaccgg ataaattaat agcgtctcgc
     4321 tggcgagatc ttccggtgta atacgcgttt ttgccgccag tggatggtca ggtgctaaca
     4381 ccaggcgtac ttcataatcg aacatcggcg aataatgcag gccactgcgc ggcaggatat
     4441 cggaggtcat caccagatcc agctcgccct gttgcaacgc gggctgcggg tcaaacgtca
     4501 cgcccgattt aaaatccatc tctacctgcg gccagttctt atggaaattt tctaaagcgg
     4561 gtgtcagcca ctgaatacag ctatggcact caatggcaat gcgcagacgc gtctgctgcg
     4621 gttcattgca ggcttgcagt gcctggctga tttgcggcag cacctggttt gccagttgca
     4681 acaggatttc tccctgcggt gtaaagcgta gcggctggct cttacgcaca aatagccgga
     4741 agccaaggcg ttgttccaga tcgctaaact ggtgagacag ggcggattgc gtctgatgca
     4801 acgttgccgc agcggctgcg agcgagccgc agttccgcaa cgcttgtagc gttttcaggt
     4861 gttttacttc gatcatgaaa gtccttcact tcggcatgaa taatttgcgc ttgaggaata
     4921 tacagtaacc gccaattatg gatgtgtaaa catctggacg gctaaaatcc ttcgtctttt
     4981 aaatttatgg tgcgttggct gcgtttctcc accccggtca cttacttcag taagctcccg
     5041 gcgatgaata aacttgccgc cttccctaaa ttcaaaatcc ataggattta catataatta
     5101 gaggaagaaa aatgacaata ttgaatcaca ccctcggttt ccctcgcgtt ggcctgcgtc
     5161 gcgagctgaa aaaagtgcaa gaaagttatt gggcggggaa ctccacgcgt gaagaactgc
     5221 tgacagtagg gcgtgaactg cgtgctcgtc actgggatca acaaaagcaa gcgggtatcg
     5281 acctgctgcc ggtgggcgat tttgcctggt acgatcatgt actgaccacc agtttgctgc
     5341 tgggtaatgt tccgccacgt catcagaaca aagacggttc ggtagatatc gacaccctgt
     5401 tccgtattgg tcgtggacgt gcgccgactg gcgaacctgc ggcggcagcg gaaatgacca
     5461 aatggtttaa caccaactat cactacatgg tgccggagtt cgttaaaggc caacagttca
     5521 aactgacctg gacgcagctg ctggaggaag tggacgaggc gctggcgctg agccacaacg
     5581 ttaagcctgt gctgctgggg ccggttactt acctgtggct ggggaaagtg aaaggtgaac
     5641 agtttgaccg ccttagcctg ctgaacgaca ttctgccggt ttatcagcaa gtgctggcag
     5701 aactggcgaa acgcggcatc gagtgggtac agattgatga acccgcgctg gtactggaac
     5761 taccacaggc gtggctggac gcatacaaac ccgcttacga cgcgctccag ggacaggtga
     5821 aactgctgct gaccacctat tttgaaggcg taacgccaaa tctcgacacg attactgcgc
     5881 tgcctgttca ggggctgcat gtggaccttg tacatggtaa agatgacgtt gttgaactgc
     5941 acaagcgcct gccttctgac tggctgctgt ctgcgggtct gatcaatggt cgtaacgtct
     6001 ggcgcgccga tcttactgag aaatatgcgc aaattaagga cattgtcggc aaacgtgatt
     6061 tgtgggtggc atcttcctgc tcgttgctgc acagccccat cgacctgagc gtggaaacgc
     6121 gtcttgatgc agaagtgaaa agctggtttg ccttcgccct acaaaaatgc catgaactgg
     6181 cactgctgcg cgatgcgctg aacagtggtg acacggcagc tctggcagag tggagcgccc
     6241 caattcaggc gcgtcgtcac tctactcgtg tacataatcc ggcggtagaa aagcgtctgg
     6301 cggcgatcac cgcccaggac agccagcgtg cgaatgtcta tgaagtgcgt gctgaagccc
     6361 agcgtgcgcg ttttaaactg ccagcgtggc cgaccaccac gattggttcc ttcccgcaaa
     6421 ccacggaaat tcgtaccctg cgtctggatt tcaaaaaggg caatctcgac gccaacaact
     6481 accgcacggg cattgcggaa catatcaagc aggccattgt tgagcaggaa cgtttgggac
     6541 tggatgtgct ggtacatggc gaggccgagc gtaatgacat ggtggaatac tttggcgagc
     6601 acctcgacgg atttgtcttt acgcaaaacg gttgggtaca gagctacggt tcccgctgcg
     6661 tgaagccacc gattgtcatt ggtgacatta gccgcccggc accgattacc gtggagtggg
     6721 cgaagtatgc gcaatcgctg accgacaaac cggtgaaagg gatgctgacg gggccggtga
     6781 ccatactctg ctggtcgttc ccgcgtgaag atgtcagccg tgaaaccatc gccaaacaga
     6841 ttgcgctggc gctgcgtgat gaagtggccg atctggaagc cgctggaatt ggcatcatcc
     6901 agattgacga accggcgctg cgcgaaggtt taccgctgcg tcgtagcgac tgggatgcgt
     6961 atctccagtg gggcgtagag gccttccgta tcaacgccgc cgtggcgaaa gatgacacac
     7021 aaatccacac tcacatgtgt tattgcgagt tcaacgacat catggattcg attgcggcgc
     7081 tggacgcaga cgtcatcacc atcgaaacct cgcgttccga catggagttg ctggagtcgt
     7141 ttgaagagtt tgattatcca aatgaaatcg gtcctggcgt ctatgacatt cactcgccaa
     7201 acgtaccgag cgtggaatgg attgaagcct tgctgaagaa agcggcaaaa cgcattccgg
     7261 cagagcgcct gtgggtcaac ccggactgtg gcctgaaaac gcgcggctgg ccagaaaccc
     7321 gcgcggcact ggcgaacatg gtgcaggcgg cgcagaattt gcgtcggggg taaatccaaa
     7381 ccgggtggta ataccacccg gtcttttctc attacagcga cttcttccca ccatactgct
     7441 taaaccattc cagcatacgc tgccagccat ctttcgcaga ttcggcatga tagctcgggc
     7501 gataatcagc gttgaatgca tgcccggcat ccgggtacac gataatctct gctttcgcat
     7561 tagcagcccg cagcgcctgg cgcatcgttt caacgctctc ctgcggaatg ctgttatcct
     7621 gaccaccata taagccgaga accggcgcgt taagatcggt tgcgatatca acaggttgtt
     7681 tcggtgaatt cagcgacttg tcgcccgtca gtttgccgta ccacgccact gcggctttta
     7741 gctgtggatt atgcgcggca tacagccagg tgatacgtcc accccagcag aatccggtga
     7801 tcattaaacg atgaacatcg ccgccgttgc gggaagccca actggcgaca tgatcgagat
     7861 cggccagcac ctgcgagtca ggcacttttg ctaccagacc gctaagcaac gtggggatat
     7921 cggcaaaatc attcggatcg ccttcgcgga agtaaagttc aggtgcgata gccagatacc
     7981 cctccagcgc cagacggcga caaatgtcgc ggatatgttc atgcacgcca aaaatttcct
     8041 gcactacaat gaccactggc agtgggccat cgctttgctt tggtctggca tggtaagcag
     8101 gcatgttatc cccttgtgaa gggatagagg tgaagcccgc cacaatcgcg tcgtccgggg
     8161 tctgaacgat ggtcgaagcg agaggcgatg cagcaggtgc aaatccagat tgttgtgttg
     8221 ttgccatggt attctccgta cccttataaa aatgttgcgc aatgttaact atagtcagca
     8281 tgcaacaaat cacattgcct gaatcggctc atcttttatg cagtcctgca gaatgaagag
     8341 tgatttatgt gatatgaatc acttttggtg ggtaaattta tgcaacgcat ttgcgtcatg
     8401 gtgatgagta tcacgaaaaa atgttaaacc cttcggtaaa gtgtcttttt gcttcttctg
     8461 actaaaccga ttcacagagg agttgtatat gtccaagtct gatgtttttc atctcggcct
     8521 cactaaaaac gatttacaag gggctacgct tgccatcgtc cctggcgacc cggatcgtgt
     8581 ggaaaagatc gccgcgctga tggataagcc ggttaagctg gcatctcacc gcgaattcac
     8641 tacctggcgt gcagagctgg atggtaaacc tgttatcgtc tgctctaccg gtatcggcgg
     8701 cccgtctacc tctattgctg ttgaagagct ggcacagctg ggcattcgca ccttcctgcg
     8761 tatcggtaca acgggcgcta ttcagccgca tattaatgtg ggtgatgtcc tggttaccac
     8821 ggcgtctgtc cgtctggatg gcgcgagcct gcacttcgca ccgctggaat tcccggctgt
     8881 cgctgatttc gaatgtacga ctgcgctggt tgaagctgcg aaatctattg gcgcgacaac
     8941 tcacgttggc gtgacagttt cttctgatac cttctaccca ggtcaggaac gttacgatac
     9001 ttactctggt cgcgtagttc gtcactttaa aggttctatg gaagagtggc aggcgatggg
     9061 cgtaatgaac tatgaaatgg aatctgcaac tctgctgacc atgtgtgcaa gtcagggcct
     9121 gcgtgccggt atggtagcgg gtgttatcgt taaccgcacc cagcaagaga tcccgaatgc
     9181 tgagacgatg aaacaaactg aaagccatgc ggtgaaaatc gtggtggaag cggcgcgtcg
     9241 tctgctgtaa ttctcttctc ctgtctgaag gccgacgcgt tcggcctttt gtatttttgc
     9301 gtagcgcctc gcaggaaatg cctttccaac tggacgtttg tacagcacaa ttctattttg
     9361 tgcgggtaag ttgttgcgtc aggaggcgtt gtggatttct caatcatggt ttacgcagtt
     9421 attgcgttgg tgggtgtggc aattggctgg ctgtttgcca gttatcaaca tgcgcagcaa
     9481 aaagccgagc aattagctga acgtgaagag atggtcgcgg agttaagcgc ggcaaaacaa
     9541 caaattaccc aaagcgagca ctggcgtgca gagtgtgaat tgctcaataa cgaagtgcgc
     9601 agcctgcaaa gtattaacac ctctctggag gccgatctgc gtgaagtaac cacgcggatg
     9661 gaagccgcac agcaacatgc tgacgataaa attcgccaga tgattaacag cgagcagcgc
     9721 ctcagtgagc agtttgaaaa cctcgccaac cgtatttttg agcacagcaa tcgccgggtt
     9781 gatgagcaaa accgtcagag tctgaacagc ctgttgtcgc cgctacgtga acaactggac
     9841 ggtttccgcc gtcaggttca ggacagcttc ggtaaagaag cacaagaacg ccataccctg
     9901 acccacgaaa ttcgcaatct ccagcaactc aacgcgcaaa tggcccagga agcgatcaac
     9961 ctgacgcgcg cgctgaaagg cgacaataaa acccagggca actggggcga ggtagtattg
    10021 acgcgggtgc tggaggcttc cggtctgcgt gaagggtatg aatatgaaac ccaggtcagc
    10081 atcgaaaatg acgcccgctc gcggatgcag ccggatgtca tcgtgcggct gccgcaggga
    10141 aaagatgtgg tgatcgacgc caaaatgacg ctggtcgcct atgaacgcta ttttaacgcc
    10201 gaagacgact acacccgcga aagcgcgcta caggaacata tcgcgtcggt gcgtaaccat
    10261 atccgtttgc tgggacgcaa agattatcaa cagctgccgg ggctgcgaac tctggattac
    10321 gtgctgatgt ttattcccgt tgaacccgct tttttactgg cgcttgaccg ccagccggag
    10381 ctgatcaccg aagcgttgaa aaacaatatc atgctggtta gcccgactac gctgctggtg
    10441 gcgttgcgta ctattgccaa cctgtggcgt tatgagcatc aaagccgtaa cgcccagcaa
    10501 atcgccgatc gtgccagcaa gctgtacgac aagatgcgtt tgttcatcga tgacatgtcc
    10561 gcgattggtc aaagtctcga caaagcgcag gataactacc gccaggcaat gaaaaaactc
    10621 tcttcaggtc gcggaaatgt gctggcgcag gcagaagcgt ttcgcggttt aggagtagaa
    10681 attaaacgcg agattaatcc ggatttggct gaacaggcgg tgagccagga tgaagagtat
    10741 cgacttcggt cggttccggg gcagccgaat gatgaagctt atcaacgcga tgatgaatat
    10801 aatcagcagt cgcgctagcc cattaggggt agttaagccg ggtagaaatc tagggcatcg
    10861 acgcccaatc tgttacactt ctggaacaat tttttgatga gcaggcattg agatggtgga
    10921 taagtcacaa gaaacgacgc actttggttt tcagaccgtc gcgaaggaac aaaaagcgga
    10981 tatggtcgcc cacgttttcc attccgtggc atcaaaatac gatgtcatga atgatttgat
    11041 gtcatttggt attcatcgtt tgtggaagcg attcacgatt gattgcagcg gcgtacgccg
    11101 tgggcagacc gtgctggatc tggctggtgg caccggcgac ctgacagcga aattctcccg
    11161 cctggtcgga gaaactggca aagtggtcct tgctgatatc aatgaatcca tgctcaaaat
    11221 gggacgcgag aagctgcgta atatcggtgt gattggcaac gttgagtatg ttcaggcgaa
    11281 cgctgaggcg ctgccgttcc cggataacac ctttgattgc atcaccattt cgtttggtct
    11341 gcgtaacgtc accgacaaag ataaagcact gcgttcaatg tatcgcgtgc tgaaacccgg
    11401 cggccgcctg ctggtgcttg agttctcgaa gccaattatc gagccgctga gcaaagccta
    11461 tgatgcatac tccttccatg tgctgccgcg tattggctca ctggtcgcga acgacgccga
    11521 cagctaccgt tatctggcag aatccatccg tatgcatccc gatcaggata ccctgaaagc
    11581 catgatgcag gatgccggat tcgaaagtgt cgactactac aatctgacgg caggggttgt
    11641 ggcgctgcat cgtggttata agttctgaca ggagaccgga aatgcctttt aaacctttag
    11701 tgacggcagg aattgaaagt ctgctcaaca ccttcctgta tcgctcaccc gcgctgaaaa
    11761 cagcccgctc gcgtctgctg ggtaaagtat tgcgcgtgga ggtaaaaggc ttttcgacgt
    11821 cattgattct ggtgttcagc gaacgccagg ttgatgtact gggcgaatgg gcaggcgatg
    11881 ctgactgcac cgttatcgcc tacgccagtg tgttgccgaa acttcgcgat cgccagcagc
    11941 ttaccgcact gattcgcagt ggtgagctgg aagtgcaggg cgatattcag gtggtgcaaa
    12001 acttcgttgc gctggcagat ctggcagagt tcgaccctgc ggaactgctg gccccttata
    12061 ccggtgatat cgccgctgaa ggaatcagca aagccatgcg cggaggcgca aagttcctgc
    12121 atcacggcat taagcgccag caacgttatg tggcggaagc cattactgaa gagtggcgta
    12181 tggcacccgg tccgcttgaa gtggcctggt ttgcggaaga gacggctgcc gtcgagcgtg
    12241 ctgttgatgc cctgaccaaa cggctggaaa aactggaggc taaatgacgc ctggtgaagt
    12301 acggcgccta tatttcatca ttcgcacttt tttaagctac ggacttgatg aactgatccc
    12361 caaaatgcgt atcaccctgc cgctacggct atggcgatac tcattattct ggatgccaaa
    12421 tcggcataaa gacaaacttt taggtgagcg actacgactg gccctgcaag aactggggcc
    12481 ggtttggatc aagttcgggc aaatgttatc aacccgccgc gatctttttc caccgcatat
    12541 tgccgatcag ctggcgttat tgcaggacaa agttgctccg tttgatggca agctggcgaa
    12601 gcagcagatt gaagctgcaa tgggcggctt gccggtagaa gcgtggtttg acgattttga
    12661 aatcaagccg ctggcttctg cttctatcgc ccaggttcat accgcgcgat tgaaatcgaa
    12721 tggtaaagag gtggtgatta aagtcatccg cccggatatt ttgccggtca ttaaagcgga
    12781 tctgaaactt atctaccgtc tggctcgctg ggtgccgcgt ttgctgccgg atggtcgccg
    12841 tctgcgccca accgaagtgg tgcgcgagta cgaaaagacc ttgattgatg aactgaattt
    12901 gctgcgggaa tctgccaacg ccattcagct tcggcgcaat tttgaagaca gcccgatgct
    12961 ctacatcccg gaagtttacc ctgactattg tagtgaaggg atgatggtga tggagcgcat
    13021 ttacggcatt ccggtgtctg atgttgcggc gctggagaaa aacggcacta acatgaaatt
    13081 gctggcggaa cgcggcgtgc aggtgttctt cactcaggtc tttcgcgaca gctttttcca
    13141 tgccgatatg caccctggca acatcttcgt aagctatgaa cacccggaaa acccgaaata
    13201 tatcggcatt gattgcggga ttgttggctc gctaaacaaa gaagataaac gctatctggc
    13261 agaaaacttt atcgccttct ttaatcgcga ctatcgcaaa gtggcagagc tacacgtcga
    13321 ttctggctgg gtgccaccag ataccaacgt tgaagagttc gaatttgcca ttcgtacggt
    13381 ctgtgaacct atctttgaga aaccgctggc cgaaatttcg tttggacatg tactgttaaa
    13441 tctgtttaat acggcgcgtc gcttcaatat ggaagtgcag ccgcaactgg tgttactcca
    13501 gaaaaccctg ctctatgtcg aaggggtagg acgccagctt tatccgcagc tcgatttatg
    13561 gaaaacagcg aagcctttcc tggagtcgtg gattaaagat caggtcggta ttcctgccct
    13621 ggtgagagca tttaaagaaa aagcgccgtt ctgggtcgaa aaaatgccag aactgcctga
    13681 actggtttac gacagtttgc ggcagggcaa gtatttacag caccgtgttg ataagattgc
    13741 ccgcgagctt cagtcaaatc atgtacgtca gggacaatcg cgttattttc tcggaattgg
    13801 cgctacgtta gtattaagtg gcacattctt gttggtcagc cgacctgagt gggggctgat
    13861 gcccggctgg ttaatggcag gtggtctgat cgcctggttt gtcggttggc gcaaaacacg
    13921 ctgatttttt catcgctcaa ggcgggccgt gtaacgtata atgcggcttt gtttaatcat
    13981 catctaccac agaggaacat gtatgggtgg tatcagtatt tggcagttat tgattattgc
    14041 tgtcatcgtt gtactgcttt ttggcaccaa aaagctcggc tccatcggtt ccgatcttgg
    14101 tgcgtcgatc aaaggcttta aaaaagcaat gagcgatgat gaaccaaagc aggataaaac
    14161 cagtcaggat gctgatttta ctgcgaaaac tatcgccgat aagcaggcgg atacgaatca
    14221 ggaacaggct aaaacagaag acgcgaagcg ccacgataaa gagcaggtgt aatccgtgtt
    14281 tgatatcggt tttagcgaac tgctattggt gttcatcatc ggcctcgtcg ttctggggcc
    14341 gcaacgactg cctgtggcgg taaaaacggt agcgggctgg attcgcgcgt tgcgttcact
    14401 ggcgacaacg gtgcagaacg aactgaccca ggagttaaaa ctccaggagt ttcaggacag
    14461 tctgaaaaag gttgaaaagg cgagcctcac taacctgacg cccgaactga aagcgtcgat
    14521 ggatgaacta cgccaggccg cggagtcgat gaagcgttcc tacgttgcaa acgatcctga
    14581 aaaggcgagc gatgaagcgc acaccatcca taacccggtg gtgaaagata atgaagctgc
    14641 gcatgagggc gtaacgcctg ccgctgcaca aacgcaggcc agttcgccgg aacagaagcc
    14701 agaaaccacg ccagagccgg tggtaaaacc tgctgcggac gcggaaccga aaaccgctgc
    14761 accttcccct tcgtcgagtg ataaaccgta aacatgtctg tagaagatac tcaaccgctt
    14821 atcacgcatc tgattgagct gcgtaagcgt ctgctgaact gcattatcgc ggtgatcgtg
    14881 atattcctgt gtctggtcta tttcgccaat gacatctatc acctggtatc cgcgccattg
    14941 atcaagcagt tgccgcaagg ttcaacgatg atcgccaccg acgtggcctc gccgttcttt
    15001 acgccgatca agctgacctt tatggtgtcg ctgattctgt cagcgccggt gattctctat
    15061 caggtgtggg catttatcgc cccagcgctg tataagcatg aacgtcgcct ggtggtgccg
    15121 ctgctggttt ccagctctct gctgttttat atcggcatgg cattcgccta ctttgtggtc
    15181 tttccgctgg catttggctt ccttgccaat accgcgccgg aaggggtgca ggtatccacc
    15241 gacatcgcca gctatttaag cttcgttatg gcgctgttta tggcgtttgg tgtctccttt
    15301 gaagtgccgg tggcaattgt gctgctgtgc tggatgggga ttacctcgcc agaagactta
    15361 cgcaaaaaac gcccgtatgt gctggttggt gcattcgttg tcgggatgtt gctgacgccg
    15421 ccggatgtct tctcgcaaac gctattggcg atcccgatgt actgtctgtt tgaaatcggt
    15481 gtcttcttct cacgctttta cgttggtaaa gggcgaaatc gggaagagga aaacgacgct
    15541 gaagcagaaa gcgaaaaaac tgaagaataa attcaaccgc ccgtcagggc ggttgtcata
    15601 tggagtacag gatgtttgat atcggcgtta atttgaccag ttcgcaattt gcgaaagacc
    15661 gtgatgatgt tgtagcgcgc gcttttgacg cgggagttaa tgggctactc atcaccggca
    15721 ctaacctgcg tgaaagccag caggcgcaaa agctggcgcg tcagtattcg tcctgttggt
    15781 caacggcggg cgtacatcct cacgacagca gccagtggca agctgcgact gaagaagcga
    15841 ttattgagct ggctgcgcag ccagaagtgg tggcgattgg tgaatgtggt ctcgacttta
    15901 accgcaactt ttcgacgccg gaagagcagg aacgcgcttt tgttgctcag ctacgcattg
    15961 ccgcagaatt aaacatgccg gtatttatgc actgtcgcga tgcccacgag cggtttatga
    16021 cattgctgga gccgtggctg gataaactgc ctggtgcggt tcttcattgc tttaccggca
    16081 cacgcgaaga gatgcaggcg tgcgtggcgc gtggaattta tatcggcatt accggttggg
    16141 tttgcgatga acgacgcggg ctggagctgc gggaattgtt gccgttgatt ccggcggaga
    16201 aattgctgat cgaaacagat gcgccgtatc tgctcccgcg cgatctcacg ccaaagccat
    16261 catcccggcg caacgagcca gcccatctgc cccatatttt gcaacgtatt gcgcactggc
    16321 gtggagaaga tgccgcatgg ctggctgcca ccacggatgc caatgtcaaa acactgtttg
    16381 ggattgcgtt ttagagtttg cggaactcgg tgttcttcac actgtgctta atctctttat
    16441 taataagatt aagcaatagc atggagcgag cctcaccatc gggttcggtg aaaatggcct
    16501 gaaagccttc gaacgcgcct tcggtaataa tcaccttatc acccggataa gggggggccg
    16561 gatcgacaat gtctttcggt ttatataccg atagctgatg aatcaccgcc gatgggacta
    16621 tcgctggcga cgcgccaaag cgcacaaagt ggctgacgcc acgcgtagcg ctgatggtcg
    16681 tggtatgaat cacttctgga tcaaattcca caaacaggta gttagggaat aacggttcac
    16741 tgactgcggt acgtttaccc cgtacgattt tttccagggt gatcatcgga gcgaggcaat
    16801 taaccgcctg tctctcaagg tgttcctggg cacgttgaag ttgcccgcgc ttgcagtaca
    16861 gtaaatacca ggattgcata atgactctta tccgtttaat cggggcgcaa ggatagcaaa
    16921 agctttacgc taagttaatt atattccccg gtttgcgtta taccgtcaga gttcacgcta
    16981 atttaacaaa tttacagcat tgcaaagatg aacgccgtat aatgggcgca gattaagagg
    17041 ctacaatgga cgccatgaaa tataacgatt tacgcgactt cctgacgctg cttgaacagc
    17101 agggtgagct aaaacgtatc acgctcccgg tggatccgca tctggaaatc actgaaattg
    17161 ctgaccgcac tctgcgtgct ggtgggcctg cgctgttgtt cgaaaaccct aaaggctact
    17221 caatgccggt gctgtgcaac ctgttcggta cgccaaagcg cgtggcgatg ggcatggggc
    17281 aggaagatgt ttcggcgctg cgtgaagttg gtaaattatt ggcgtttctg aaagagccgg
    17341 agccgccaaa aggtttccgc gacctgtttg ataaactgcc gcagtttaag caggtgttaa
    17401 acatgccgac aaagcgactg cgtggtgcgc cctgccaaca aaaaatcgtc tctggcgatg
    17461 acgtcgatct caatcgcatt cccattatga cctgctggcc ggaagatgcc gcgccgctga
    17521 ttacctgggg gctgaccgta acgcgcggcc cgcataaaga gcggcagaat ctgggcattt
    17581 atcgccagca gctgattggt aaaaacaaac tgattatgcg ctggctgtcg catcgcggcg
    17641 gcgcgctgga ttatcaggag tggtgtgcgg cgcatccggg cgaacgtttc ccggtttctg
    17701 tggcgctggg tgccgatcct gccacgattc tcggtgcagt cacccccgtt ccggatacgc
    17761 tttcagagta tgcgtttgcc ggattgctac gcggcaccaa aaccgaagta gtaaagtgta
    17821 tttccaatga cctcgaagtg ccagccagtg ccgaaatcgt cctggaaggg tatatcgatc
    17881 ctggtgagat ggcgccggaa gggccgtatg gtgaccacac aggttactat aatgaagtcg
    17941 atagtttccc ggtggttacc gtgacgcata ttacccagcg tgaagatgcg atttaccatt
    18001 ccacctatac cgggcgtccg ccagatgagc ccgcggtact gggcgtggcg ttgaacgaag
    18061 tgtttgtacc gattctgcaa aaacagttcc cggaaattgt cgatttttac ctgccgccgg
    18121 aaggctgttc ttatcgtctg gcggtagtga cgatcaaaaa acagtacgcc ggacacgcga
    18181 agcgcgtcat gatgggcgtc tggtcgttct tacgccagtt tatgtacact aaatttgtga
    18241 tcgtttgcga tgatgacgtc aacgcacgcg actggaacga tgtgatttgg gcgattacca
    18301 cccgtatgga cccggcgcgg gatactgttc tggtagaaaa tacgcctatt gattatctgg
    18361 attttgcctc gcctgtctct gggctgggtt caaaaatggg gctggatgcc acgaataaat
    18421 ggccggggga aacccagcgt gaatggggac gtcccatcaa aaaagatcca gatgttgtca
    18481 cgtatattga cgccatctgg gatgaactgg ctatttttaa caacggtaaa agcgcctgat
    18541 gcgcgtttgt tttgccctat ttatcgatcc gacagagaaa gcgcatgaca accttaagct
    18601 gtaaagtgac ctcggtagaa gctatcacgg ataccgtata tcgtgtccgc atcgtgccag
    18661 acgcggcctt ttcttttcgt gctggtcagt atttgatggt agtgatggat gagcgcgaca
    18721 aacgtccgtt ctcaatggcc tcgacgccgg atgaaaaagg gtttatcgag ctgcatattg
    18781 gcgcttctga aatcaacctt tacgcgaaag cagtcatgga ccgcatcctc aaagatcatc
    18841 aaatcgtggt cgacattccc cacggagaag cgtggctgcg cgatgatgaa gagcgtccga
    18901 tgattttgat tgcgggcggc accgggttct cttatgcccg ctcgattttg ctgacagcgt
    18961 tggcgcgtaa cccaaaccgt gatatcacca tttactgggg cgggcgtgaa gagcagcatc
    19021 tgtatgatct ctgcgagctt gaggcgcttt cgttaaagca ccctggtctg caagtggtgc
    19081 cggtggttga acaaccggaa gcaggctggc gtgggcgtac tggtaccgtg ttaacggcgg
    19141 tattgcagga tcatggtacg ctggcagagc atgatatcta tattgccgga cgttttgaga
    19201 tggcgaaaat tgcccgtgac ctgttttgca gtgagcgtaa tgcgcgggaa gatcgcctgt
    19261 ttggcgatgc gtttgcattt atctgagata taaaaaaacc cgcccctgac aggcgggaag
    19321 aacggcaact aaaactttat tcagtggcat ttagatctat gtcgcatccg gcaagtggtt
    19381 aaacccgctc aaacaccgtc gcaataccct gacccagacc gatacacatc gtcgccagac
    19441 caaactgaac gtctttgcgt tccatcagat tcagcagcgt ggtgctgata cgcgcaccgg
    19501 aacaacccag cggatgaccc agcgcgatcg caccaccatt gaggttgatc ttctcgtcaa
    19561 tctgctccat tagcccgaga tctttaatgc acggcaggat ctgcgcggca aaggcttcgt
    19621 tcatctcaaa cacgccgata tcgctggcag aaagccccgc ttttttcagc gccagcttcg
    19681 aggccggaac cgggccgtaa cccataatcg atgggtcaca accaacgacc gccatcgaac
    19741 gcacacgagc gcgcggctta agacctaatt catgggcgcg gctttcactc atcaccagca
    19801 tggcagctgc gccatcggaa agtgcagaag atgtgcccgc cgttaccgta ccgtttactg
    19861 gatcaaacgc cggacgcagc gtggcgaggg cttccacggt ggtttccggg cgaatcactt
    19921 cgtcgtaatt aaactgcttc aggacgccgt cggcatcgtg accaccagtt gggatgattt
    19981 catttttaaa tgcggccgac tgcgtggcgg cccaggcgcg ggcgtgtgac cgcgcggcaa
    20041 aggcatcctg catttcacgg ctgataccgt gtatacgcgc cagcatttct gccgttaagc
    20101 ccatcatgcc cgccgctttg gcgacattgc ggctcaggcc ggggtgaaaa tcgacgccgt
    20161 gactcatcgg cacatggccc atatgctcca cgccgccaac cagacatgcc tgcgcatcgc
    20221 cagtcatgat cattcgtgct gcgtcatgca gtgcctgcat ggatgaacca cacaagcgat
    20281 tgacggtaac cgccgggaca gagtgcggca cttctgccag cagcgccgcg ttacgggcga
    20341 tgttaaaacc ctgctccagc gtctgttgca cacaacccca gtaaatatcg tcgagggccg
    20401 ccgcttccag cgccgggtta cgcgccagca ggctacgcat taaatgagcg gagagatctt
    20461 ccgcacgcac gttacgaaaa gcaccgccct tcgaacggcc catcggggtg cgaattgcat
    20521 cgacaatgac aacctgttcc attgtgattc cttaagccgt tttcaggtcg ccaaccggac
    20581 gggctggctc aaccggagga tagtacggtt cgttatgacg cgctttatta cgcagacctt
    20641 ccggcacttc atacagcggg ccgaggtgct gatattgctg tgccatatcg aggtattttg
    20701 cgctaccgag ggtgtccagc cagcggaacg cgccgccgtg gaacggaggg aagcccaggc
    20761 cgtagaccag cgccatatcc gcttccgccg gagtggcgat aatgccctct tccagacagc
    20821 gcaccacttc gttgaccatc gggatcatca tgcgggcgat aatctcttct tcgctgaaat
    20881 cgcgcttcgg ttggctcact tctgccagca ggtcttcaac ggcggcgtct tcttctttct
    20941 tcggcttacc tttgctgtct tctttataac gccagaaacc gaggccgttc ttctgaccaa
    21001 agcggttggc atcaaacagc gcgtcgatgg catcgcggta atctttctgc atccgctgcg
    21061 ggaagcctgc tgccatgaca gcctgagcgt gatgcgcggt atcaatgccc acaacgtcca
    21121 gcagatatgc cgggcccatc ggccagccaa actgtttttc catcactttg tcgatcttgc
    21181 ggaaatccgc gccgtcgcgc agcagctggc tgaaaccggc gaaatacggg aacagcacgc
    21241 ggttaacaaa gaagccgggg cagtcgttaa ccacaatcgg cgtcttgccc atcttgctcg
    21301 cccaggcgac aactttcgcg atggtttcgt cggagctttt ctcgccgcga ataatttcta
    21361 ccaacggcat tcggtggacc gggttaaaga agtgcatccc gcagaagttt tccgggcgtt
    21421 ccagcgcgtt ggccagttcg ctgataggaa tggttgaagt gttagacgcc agcacggtat
    21481 cctggcgtac tttttgttcg gtttctgcca gtacggcttt tttcactttc gggttttcaa
    21541 caaccgcttc taccacaata tccacgcggt caaatccggc gtagtcgagc gttgggtgga
    21601 tcgtggagat cacgccagcc agtttcagac catcgatctt gccgcgctca agctgcttgt
    21661 tcagcagttt cgcggcttcg gtcatgccga gggttaacga cttgtcgttg atatctttca
    21721 tgacaaccgg cacgcctttc cacgcggact ggtaagcgat accgccaccc atgatgcctg
    21781 cgcccagcac agcagcctgt ttcggggttt caacgtcttt ggtgagtttc ttcgctttgc
    21841 cttttacata ttgatcgtta aggaaaatgc cgaccagtgc gcgagcttcg ttggtatgcg
    21901 ccagcgggac aaaacttttg ttttccagat ttaaggcttc ttcacgacca aaacgggccg
    21961 cagcttcaat ggtttttact gcggtgatgg gggccggata atgtttcccc gccgtttgcg
    22021 cgaccattcc tttagcgatg gtgaagctca tggtggcttc aatcttgctc agttttagcg
    22081 gttccagctt cggctgacgt ttcgctttcc agtcgaggtc gccgttaatg gcctggcgta
    22141 aaatcgccat cgccccttca atcagttttt ctgctttgac tacgccatcc accagaccga
    22201 ttttcagcgc ctgatccgcg ccgacatctt taccggcggc agtgatttcc agtgcactgt
    22261 cagcgcccag catacgtggc atacgtacag aaccgccaaa gcccggcatg atgcccagtt
    22321 tggtttccgg cagaccgatg cgcagatccg gcgtcgccag acgataatcg gtcgccagca
    22381 cgcattcgca gccaccaccc agcgcatagc cgttgacggc agcaatggtc ggcaccggca
    22441 gatcttccag gcgattaaac acgctattgg caaagtgcag ccactgactt aactgttctt
    22501 caggaacgag gaacagggac aaaaattcgg tgatatcagc accgacgata aaggctgctt
    22561 tgttcgaacg cagcagcagc ccttttagat ctgattgctg ttccagcacg ccgatggcct
    22621 cgccgaggct ggcgacggtc gcagtgtcga gtttattaac tgaacctggg gcatcaaata
    22681 ccagttcggc aatgccatct tccagccagt caaggtacag ggtgtcgcct ttgtaaagca
    22741 tgtcagtctc ctgaatccgc aaggtgatct ggtcgtacca gatgcatcgg agtgtgtgtt
    22801 ttgtgttaaa aatatgcaaa taaaagatta aagaaatgcc gatctgatca cgctcggcag
    22861 aaatcacgct ctggatgaac gatgtgctaa gatgcggaga cttaaggtca aaaaaacaga
    22921 agggtaaaaa atggaatcac tggcctcgct ctataaaaat catatagcta ccttacaaga
    22981 acggactcgc gatgcgctgg cgcgcttcaa gctggatgca ttacttattc actccggcga
    23041 gctgttcaac gtttttctcg acgatcatcc ctatccgttt aaagtgaacc cgcaattcaa
    23101 agcgtgggtg ccggtaactc aggtgccaaa ctgctggttg ctggtggatg gcgtgaacaa
    23161 gccgaaactg tggttctatc tgccggttga ttactggcac aacgtcgaac cgctgccgac
    23221 ctccttctgg actgaagatg tggaagtgat cacgctgccg aaagccgatg gcattggtag
    23281 cctgctgcct gctgcgcgcg gcaatatcgg ttatatcggt ccggtgccgg aacgtgcgct
    23341 gcaactgggt attgaggcca gcaacatcaa cccgaaaggg gtgatcgact acctgcatta
    23401 ttaccgctcc ttcaaaaccg agtacgaact ggcctgtatg cgtgaagcgc agaaaatggc
    23461 ggtcaatggt catcgtgcgg cagaagaagc gttccgttct ggcatgagcg agttcgatat
    23521 caatattgcc tatctgaccg cgaccggtca tcgtgatacc gacgtacctt acagcaacat
    23581 tgtggcgctt aacgaacacg ctgcggtgtt gcattacacc aaactggacc accaggcacc
    23641 ggaagagatg cgcagcttcc tgctggatgc cggggccgaa tataacggct atgcggctga
    23701 cctgacccgt acctggtcgg caaaaagcga caacgactac gcacagctgg tgaaagacgt
    23761 aaatgatgaa caactggggc tgatcgccac catgaaagca ggcgtcagct atgtggatta
    23821 ccacatccag ttccatcagc gcatcgccaa actgctgcgt aaacatcaaa tcatcaccga
    23881 tatgagtgaa gaagcgatgg tcgaaaacga tctcaccgga ccgtttatgc cgcacggtat
    23941 tggccatccg ctgggcctgc aggtgcatga cgtcgccggt tttatgcagg atgatagcgg
    24001 tacacacctc gcggcaccgg caaaatatcc gtacctgcgc tgcacccgta ttctccagcc
    24061 gggcatggtg ttaaccatcg aaccgggtat ctacttcatt gaatcgcttc tggcaccgtg
    24121 gcgtgaaggg cagttcagca agcacttcaa ctggcagaaa attgaagcac tgaaaccgtt
    24181 cggcggcatt cgtatcgaag acaacgtggt gatccacgaa aacaacgtgg aaaacatgac
    24241 ccgggatctg aaactggcgt gatggaaagc tggttaattc ctgcggcacc ggtcacggtc
    24301 gttgaagaga tcaaaaagag ccgcttcata acgatgttgg cgcataccga tggcgttgag
    24361 gcggcgaaag cgtttgttga atcggtgcgg gcagaacacc ccgatgcccg ccaccattgc
    24421 gtggcgtggg tggcgggtgc gccggatgat tctcaacagc tgggtttctc tgacgacggg
    24481 gagccggcgg gaacggcagg taaaccgatg ctcgcccagc taatgggcag cggcgtcggg
    24541 gaaattaccg ctgtggtagt gcgctactac ggcggcatat tgcttggcac cggtgggtta
    24601 gtgaaagcgt atggcggcgg cgtgaatcag gcgctgcgcc agctaacgac ccaacgcaag
    24661 acgccattaa ccgaatatac tttgcaatgt gaatatagtc agttaaccgg cattgaagcg
    24721 ttgctggggc agtgtgacgg caaaattatc aacagtgatt atcaggcatt cgttctgctg
    24781 cgggtggcgc ttccggcggc gaaagtggct gaattttccg caaagctggc ggattttagc
    24841 cgtggttcat tgcaattgtt agcgattgaa gaataatccc cacttcgttt tgcagaacta
    24901 aggaagcggc agagatgcat tttcgcgcca ttacccgaat cgttggacta ctggtcatct
    24961 tattttcagg gaccatgatt atccctgggc tggtagcact catctaccgg gatggagcgg
    25021 gccgcgcttt tacccagacc ttttttgtcg ccctcgccat tggctctatg ctgtggtggc
    25081 cgaaccgcaa agagaagggc gaactgaaat cccgtgaggg gtttctgata gtggtgctgt
    25141 tttggaccgt gctgggtagt gtcggtgcgc tcccttttat cttctcggaa agcccgaacc
    25201 tcacgattac cgatgcgttt tttgaatctt tctctggcct gaccaccacc ggggccacta
    25261 cgctggtggg gctggattcg ctccctcatg ccatcctctt ttatcgccag atgctgcaat
    25321 ggtttggcgg gatggggatc atcgtgttag cggttgcgat actgcctatc ctcggcgtgg
    25381 gtgggatgca gctctatcgc gcagaaatgc ccggcccgct gaaagataac aaaatgcgcc
    25441 cgcgaattgc ggaaacggcg aaaactctgt ggttgattta tgtcttgctg accgtcgcct
    25501 gtgcgctggc gttgtggttt gccggaatgg atgcctttga tgccatcggc catagctttg
    25561 cgactatcgc tattggcggc ttctcgacac atgatgccag tatcggttat ttcgacagtc
    25621 cggctattaa cactatcatt gctatcttcc tgctgatctc cggctgtaac tacggtctgc
    25681 acttttcact gttaagtggg cgtagtctga aggtttattg gcgcgatccg gaatttcgca
    25741 tgtttatcgg cgtacagttt acgctggtgg ttatttgtac actcgtactg tggtttcata
    25801 atgtctacag ttcggcgctg atgacaatta accaggcgtt tttccaggtg gtatcgatgg
    25861 cgacaaccgc cgggtttacg actgacagca ttgcccgctg gccgctcttt ttgccggtac
    25921 tgcttttatg ttcagctttt atcggcggtt gcgccgggtc aacgggcggt ggcctgaaag
    25981 tgatccgcat cctgctgctg tttaagcagg ggaaccgtga gctgaaacga ctggtgcatc
    26041 cgaacgccgt ctatagcatt aagctgggga atcgcgcact gccggaacgt atcctcgaag
    26101 ccgtttgggg atttttctcc gcctatgcat tggtgtttat tgtcagtatg ctggcgatta
    26161 tcgccacggg cgtggatgat ttttctgcct ttgcctcagt tgttgcgaca ttgaataacc
    26221 tggggccagg gcttggcgtg gttgctgata actttaccag tatgaacccg gtggctaaat
    26281 ggatcctgat tgccaacatg ctgtttggtc gtctcgaggt ctttacattg ctggtgctct
    26341 ttaccccgac tttctggcgt gaatgatgga gtaatacgtg aaaacattaa ttcttttctc
    26401 aacaagggac ggacaaacgc gcgagattgc ctcctacctg gcttcggaac tgaaagaact
    26461 ggggatccag acggatgtcg ccaatgtgca ccgcattgaa gaaccacaat gggaaaacta
    26521 tgaccgtgtg gtcattggtg cttctattcg ctatggtcac taccactctg cgttccagga
    26581 atttgtcaaa aaacatgcga cgcgactgaa ttcgatgccg agcgcctttt attccgtaaa
    26641 cctggtggcg cgcaaaccgg agaagcgtac tccacagacc aacagctacg cgcggaagtt
    26701 tctgatgaac tcgcaatggc gtcccgatcg ctgcgcggtc attgccgggg cgctgcgtta
    26761 cccacgttat cgctggtacg accgttttat gctcaagctg attatgaaga tgtcaggcgg
    26821 tgaaacggat acgcgcaaag aagttgtcta taccgattgg gagcaggtgg cgaatttcgc
    26881 ccgagaaatc gcccatttaa ccgacaaacc gacgctgaaa taagcataaa gaa
//
LOCUS       DAETPZ010000051        26411 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.051, whole genome shotgun sequence.
ACCESSION   DAETPZ010000051 DAETPZ010000000
VERSION     DAETPZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.051"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            118..1089
                     /locus_tag="K0W59_002440"
     CDS             118..1089
                     /locus_tag="K0W59_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBI3023444.1"
                     /translation="MRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYYAGVR
                     QGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSSRTVR
                     RASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKIDQPKI
                     AVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIISTPDLN
                     AIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDISGKKM
                     GNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            1112..2623
                     /locus_tag="K0W59_002441"
     CDS             1112..2623
                     /locus_tag="K0W59_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBI3023445.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2648..3646
                     /locus_tag="K0W59_002442"
     CDS             2648..3646
                     /locus_tag="K0W59_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3023446.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNAQPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTGLSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3679..4773
                     /locus_tag="K0W59_002443"
     CDS             3679..4773
                     /locus_tag="K0W59_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBI3023447.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGAVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            4785..5657
                     /locus_tag="K0W59_002444"
     CDS             4785..5657
                     /locus_tag="K0W59_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HBI3023448.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(5705..6127)
                     /locus_tag="K0W59_002445"
     CDS             complement(5705..6127)
                     /locus_tag="K0W59_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBI3023449.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(6224..7430)
                     /locus_tag="K0W59_002446"
                     /pseudo
     CDS             complement(6224..7430)
                     /locus_tag="K0W59_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            complement(7440..8252)
                     /gene="hcaB"
                     /locus_tag="K0W59_002447"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(7440..8252)
                     /gene="hcaB"
                     /locus_tag="K0W59_002447"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HBI3023450.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVAALELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(8249..8569)
                     /locus_tag="K0W59_002448"
     CDS             complement(8249..8569)
                     /locus_tag="K0W59_002448"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HBI3023451.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(8569..9087)
                     /locus_tag="K0W59_002449"
     CDS             complement(8569..9087)
                     /locus_tag="K0W59_002449"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HBI3023452.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(9084..10445)
                     /locus_tag="K0W59_002450"
     CDS             complement(9084..10445)
                     /locus_tag="K0W59_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HBI3023453.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            10581..11471
                     /gene="hcaR"
                     /locus_tag="K0W59_002451"
     CDS             10581..11471
                     /gene="hcaR"
                     /locus_tag="K0W59_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HBI3023454.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            11631..12770
                     /locus_tag="K0W59_002452"
     CDS             11631..12770
                     /locus_tag="K0W59_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HBI3023455.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(12762..14042)
                     /gene="csiE"
                     /locus_tag="K0W59_002453"
     CDS             complement(12762..14042)
                     /gene="csiE"
                     /locus_tag="K0W59_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HBI3023456.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKHFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAVLAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(14233..15087)
                     /locus_tag="K0W59_002454"
     CDS             complement(14233..15087)
                     /locus_tag="K0W59_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI3023457.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVLRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTSPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            complement(15232..16035)
                     /gene="suhB"
                     /locus_tag="K0W59_002455"
     CDS             complement(15232..16035)
                     /gene="suhB"
                     /locus_tag="K0W59_002455"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HBI3023458.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKR"
     gene            16154..16894
                     /gene="trmJ"
                     /locus_tag="K0W59_002456"
     CDS             16154..16894
                     /gene="trmJ"
                     /locus_tag="K0W59_002456"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HBI3023459.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            17164..17652
                     /gene="iscR"
                     /locus_tag="K0W59_002457"
     CDS             17164..17652
                     /gene="iscR"
                     /locus_tag="K0W59_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HBI3023460.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            17764..18978
                     /gene="iscS"
                     /locus_tag="K0W59_002458"
     CDS             17764..18978
                     /gene="iscS"
                     /locus_tag="K0W59_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBI3023461.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            19006..19392
                     /gene="iscU"
                     /locus_tag="K0W59_002459"
     CDS             19006..19392
                     /gene="iscU"
                     /locus_tag="K0W59_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HBI3023462.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            19409..19732
                     /gene="iscA"
                     /locus_tag="K0W59_002460"
     CDS             19409..19732
                     /gene="iscA"
                     /locus_tag="K0W59_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HBI3023463.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            19828..20343
                     /gene="hscB"
                     /locus_tag="K0W59_002461"
     CDS             19828..20343
                     /gene="hscB"
                     /locus_tag="K0W59_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HBI3023464.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            20360..22210
                     /gene="hscA"
                     /locus_tag="K0W59_002462"
     CDS             20360..22210
                     /gene="hscA"
                     /locus_tag="K0W59_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HBI3023465.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            22212..22547
                     /gene="fdx"
                     /locus_tag="K0W59_002463"
     CDS             22212..22547
                     /gene="fdx"
                     /locus_tag="K0W59_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HBI3023466.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            22559..22759
                     /gene="iscX"
                     /locus_tag="K0W59_002464"
     CDS             22559..22759
                     /gene="iscX"
                     /locus_tag="K0W59_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HBI3023467.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            22818..24101
                     /gene="pepB"
                     /locus_tag="K0W59_002465"
     CDS             22818..24101
                     /gene="pepB"
                     /locus_tag="K0W59_002465"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HBI3023468.1"
                     /translation="MTEAMKITISTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            24243..25019
                     /gene="sseB"
                     /locus_tag="K0W59_002466"
     CDS             24243..25019
                     /gene="sseB"
                     /locus_tag="K0W59_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HBI3023469.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25840..26322)
                     /gene="sseA"
                     /locus_tag="K0W59_002467"
                     /pseudo
     CDS             complement(25840..26322)
                     /gene="sseA"
                     /locus_tag="K0W59_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
BASE COUNT         6256 a         6586 c         7312 g         6257 t
ORIGIN      
        1 cgttcactaa accctattaa ctgtaatatt tgagcggcac gcgctgccgc tcatcacaaa
       61 ataattagaa taatccgttg ttaataaaaa ccctacagga ggcactatgc ctaaaaaatg
      121 agaacaaccc gtaatctatt attaatggct acgctattag gtagcgcact atttgccagg
      181 gctgcggata aagaaatgac cattggtgca atataccttg atacccaggg atattacgct
      241 ggagtgcgtc agggcgttca ggatgcggca aaagattctt cagtacaggt acagttaatt
      301 gaaactaacg cccaggggga tatttcgaaa gaaagtacct ttgttgatac cctcgtggcg
      361 cgtaatgtcg atgccattat tttatcggca gtgtctgaaa atggcagtag ccgtaccgtt
      421 cgtcgcgcca gtgaagcggg tattccggtg atttgctaca acacctgtat taatcaaaag
      481 ggtgtcgata aatatgtctc ggcgtatctg gtcggcgatc cactggaatt tggtaaaaaa
      541 ctgggtaacg ctgccgccga ttattttatt gccaataaaa ttgaccagcc gaaaattgcc
      601 gtcatcaatt gcgaagcctt tgaagtttgt gtgcagcgac gtaaaggatt tgaagaagta
      661 ttaaaatccc gcgttcccgg cgcgcaaatt gtcgctaacc aggaagggac tgttttagat
      721 aaagcgattt ccgttggtga aaaactgatt atctccacgc cggatctcaa cgccattatg
      781 ggggagtcgg gcggtgcgac actcggcgcg gtaaaagcgg tacgtaatca aaatcaggcc
      841 ggaaaaattg ctgttttcgg ttcggatatg acaaccgaaa ttgctcagga gctggaaaac
      901 aatcaggtgc tgaaagcggt ggtggatatt tccggtaaga aaatgggcaa tgctgttttc
      961 gcgcaaacat tgaaggttat caataaacaa gccgacggtg aaaaagtgat tcaggtgcca
     1021 atcgatctct ataccaaaac ggaagatggt aaacagtggc tggcaacgca cgttgatggt
     1081 ctgccctaat cgctgattcg cagaggtccc tatgttcacg gcaacagagg cagtcccggt
     1141 agcaaaagtg gtggcaggaa ataagcgtta ccccggcgtc gttgcgctgg ataacgttaa
     1201 cttcacgctc aataaaggcg aagttcgtgc gctgttaggt aaaaacggcg cgggcaaatc
     1261 gactctcatt cgaatgctta ccggcagcga acgcccggat agcggtgata tctggattgg
     1321 cgagacgcga ctggaaggtg acgaagctac gttgactcgc cgtgccgctg aactgggggt
     1381 tcgtgcggtt tatcaggaat taagtctggt ggaagggctg acggtggcag aaaacctctg
     1441 ccttggtcaa tggcctcgcc gcaacggcat gattgattac ctgcaaatgg cgcaggatgc
     1501 ccaacgttgc ttacaggcgc tgggcgttga cgttagtcct gaacaacttg tttcaacgct
     1561 aagcccggcg caaaagcaac tggtggaaat tgcacgggtg atgaagggcg agccgcgcgt
     1621 ggtcattctt gatgaaccca ccagctcgct tgccagtgca gaagttgaac tggtgatcag
     1681 tgcggtgaaa aagatgtcgg cactgggcgt ggcggtgatt tatgtcagcc accggatgga
     1741 agaaatccgc cgcattgcct cctgtgccac cgttatgcgc gatggtcagg tggcgggcga
     1801 tgtgatgctc gaaaacacct ccacgcatca tattgtgtcg ctaatgctag ggcgtgatca
     1861 cgttgatatt gcgccggttg cccctcagga aattgtggat caggccgtac tggaagtccg
     1921 tgcgttacgc cataagccca agctggagga tatcagcttt acgctacgtc gtggcgaagt
     1981 gcttggcatt gctggcctgc tgggggcagg gcgcagtgaa ttgctgaagg cgattgttgg
     2041 gctggagacg tatgaacagg gcgaaattgt tatcaacggc gagaaaatca tgcgccccga
     2101 ttacggcgac atgctgaaac gcggcattgg ctatacgcca gaaaaccgca aagaagcggg
     2161 gatcattccc tggctgggcg ttgacgaaaa tacagtgctg accaatcggc aaaaaatcag
     2221 caccaacggt gtgctgcaat ggtccaccat ccgccgcctg accgaagagg tgatgcagcg
     2281 gatgacggtc aaggctgcca gtagcgaaac acccatcggc acgctttctg gtggcaatca
     2341 gcaaaaagtg gtgatcggtc gttgggtcta tgccgccagc cagattttgt tgctcgacga
     2401 gccaacgcgc ggcgtcgata tcgaagccaa acagcagatt taccgtattg tccgtgagct
     2461 ggctgccgaa ggaaaaagcg tggtgtttat ctccagtgaa gtggaggaat tgccgctggt
     2521 gtgtgaccgc atcctgttat tacagcacgg cacgttctcg caggagtttc actcgccggt
     2581 caatgtggat gagctgatgt ccgccattct gtctgtgcac tgatgaattt taccgaggaa
     2641 cctgaagatg tctgcttcgt cattaccatt gccgcagggc aagagcgtct cgctcaaaca
     2701 atttgtcagt cgccatatta atgagatcgg tttgctggtg gtgattgcca ttctttatct
     2761 ggtcttctcc ctgaacgcgc ctggctttat ctcattgaat aaccagatga acgtgctgcg
     2821 cgatgccgcc accattggga ttgccgcctg ggcgatgacg ctgattatta tctccggtga
     2881 aattgatgtc agcgttgggc cgatggtggc ttttgtctcg gtgtgcctgg catttttgct
     2941 gcaatttgaa gttccgttgg cgattgcttg tctgctggtg ttgctgttag gcgcgctgat
     3001 gggaacgctc gccggggtgc tgcgcggcgt gtttaacgta ccaagtttcg ttgccacact
     3061 ggggctgtgg agcgctctgc gcggaatggg gctgtttatg accaacgcac agccagtgcc
     3121 gattgacgaa aacgaggtgc tggactggct gggcggacaa tttctcggtg tgccagtatc
     3181 cgcgctgatc atgatggtgt tgtttgcgct gtttgtgttc attagccgaa aaaccgcctt
     3241 cgggcgctcg gtttttgccg ttggcggtaa tgccacggcg gcgcagttgt gcggtattaa
     3301 cgttcgtcga gtgcgcattc ttatctttac cctttcggga ttattagcgg cggtgaccgg
     3361 cattttgttg gcggcacgcc tcggttccgg taacgcaggt gccgcaaacg gtctggagtt
     3421 tgacgtcatc gccgcggtcg tggtcggcgg tacgggactt tccggcggtc gcggttccct
     3481 gttcggtaca ttgcttggtg tgctggtgat tacgctaatc ggtaacggtc tggtgctgct
     3541 cggtattaac tcctttttcc agcaggtggt acgcggcgtc atcatcgtgg tggcggtgct
     3601 ggcgaatatc ttgctgaccc agcgaagcag taaagcgaaa cgctaaacct taacgcccct
     3661 ggcgcgggtg cggcagggat gaaaattaat cgtattgtta atgagggatt tatgaaaacg
     3721 atgctggcag cttatttacc aggaaattca accgtcgatc tgcgggaagt tgcggtgccg
     3781 acgccgggga ttaaccaggt actgatcaaa atgaaatcct ccgggatttg cggaagcgat
     3841 gtccactata tctatcatca acaccgtgcc acggcggcgg cacccgataa accgttatac
     3901 cagggattta tcaacggtca tgaaccgtgc gggcagattg tggcgatggg gcaaggctgc
     3961 cgccatttta aagagggcga ccgcgtgctg gtgtatcaca tttctggctg tggtttttgc
     4021 ccgaactgcc gccgcggctt tcctatctct tgtactggca aaggaaaagc ggcttacggc
     4081 tggcagcgtg acggcggtca tgccgaatat ctgctggcgg aagaaaaaga tctgatcctc
     4141 ctgccggatg cgctgagcta cgaagatggt gcgtttatca gttgcggcgt tggcacggcc
     4201 tatgaaggga ttttgcgcgg cgaagtttcc ggcagcgata acgtgctggt ggtcggtctg
     4261 ggggcggtcg gcatgatggc gatgatgctg gcgaaaggtc gcggtgcaaa acggatcatc
     4321 ggcgttgata tgctgccgga acgtctggcg atggcaaaac agttaggggg gatggatcac
     4381 ggctatttag caaccaccga aggtctgccg cagataattg ctgaacttac ccacggtggc
     4441 gcggatgttg cgctcgattg ttccggtaat gccgcaggtc gcttgctggc actgcaatcc
     4501 actgctgact gggggcgggt ggtttacatt ggtgaaaccg gaaaagtgga attcgaggtc
     4561 agcgctgatc tgatgcacca tcaacggcgg attatcggct cctgggtgac cagtctgttc
     4621 catatggaaa aatgcgccca cgatttgact gactggaagc tgtggccgcg taacgccatt
     4681 acccatcgct tctcgctaga acaggcaggt gatgcctatg tgctgatggc gagcggcaaa
     4741 tgcgggaaag ttgtgattaa cttcccggat taaggagtta gcggatgacc atctatacct
     4801 tatcccacgg gtcgctaaag ctggacgttt ctgaccaggg cggcgtaatc gaaggcttct
     4861 ggcgcgatac gacgccgctc ttacgtcctg gtaaaaaaag cggtgtggca accgatgcct
     4921 catgttttcc gctggtgcca tttgcaaacc gggtgagcgg caaccgtttt gtctggcagg
     4981 ggcgtgagta tcaactgcaa ccgaatgttg agtgggatgc gcactatcta cacggcgatg
     5041 gctggctggg cgagtggcaa tgtgtctcgc acagcgatga tagtttgtgt ctggtgtatg
     5101 aacatcgcag cggtgtctat cactatcggg taagtcaggc gtttcattta acggcggata
     5161 cgctgacggt gacgctctct gtcaccaatc aaggggcaga gacgctgcca tttggtaccg
     5221 gctggcatcc ttattttccg ttgtcaccac aaacgcggat tcaggcgcag gcgagcggtt
     5281 actggctgga acgggagcag tggctggcgg gtgagttttg cgagcagcta ccgcaggaac
     5341 tggattttaa ccagctcgcg ccgttgccgc gtcagtgggt gaacaatggt tttgccggat
     5401 ggaatggtca ggcccgcatc gagcagccgc aggaaggtta tgccatcatc atggaaacga
     5461 cgccacctgc accgtgttat ttcatctttg tttccgaccc tgcgtttgat aaaggatatg
     5521 cgtttgattt cttctgtctg gaaccgatga gccatgcgcc ggatgatcat catcgcccgg
     5581 aagggggtga tctcattgcg ctagcaccag gggaatcaac aacctcagag atgtcgttgc
     5641 gggtagagtg gctgtaattt ataaaatgcc ggataaggcg ttcacgccgc atccggcatc
     5701 tttcctaacg ccgatcgaga gaaattgccc ccggtccggt aattgccaat agcaagaacg
     5761 cgccagcgat actgacattc ttccagaaat taatcatatt tggcccaacc gcatcgccgg
     5821 tcatatccca gtaatggtga ccaatcaccg ccgtacccag cgtgtagaaa ataaacagca
     5881 ccgccagcgg acgggtgaaa aagccaagca cgattaatat cgcggcgggc acttccataa
     5941 ctaccgcaat aatcgctgcc agcatcggca ttggcgcgcc caacgaggcc atatattgga
     6001 ccgtaccgtc aaagcccatc attttgggaa aaccaaaaat aatgaaaatt aagaccacgg
     6061 cgatacgggc aattaataac aaaacggggc gggcagctcc aaaatcaaaa taacgtagtg
     6121 tgttcataac caatcccttt gcaagataat gtgatttaaa cgtaatacac actcttgcac
     6181 ctcgccacgc cccaggcatt gagatattcg ctaattatcc tggttacagt gatttaagcg
     6241 cgatgttctc atctatcagc agtttcgcat caaacgtttt gccgctctgg atccatttgc
     6301 gaattgggcg aatctcacgc ccctgattca gcgtgaccgc accgataagc acgccgtttt
     6361 gcagattaaa ccaaatcgcc ttctgagttt ccgggttgcc acgacaaagc cagtcatcgc
     6421 cacgcatatc gccaataaac tgtaagttat cactgtactg atcgctccag aaccacggcg
     6481 gcggcagtag cggtagccct aacattgcgg cagcggcaat ttgcgcctgg ttattggtgt
     6541 tttcccagct ttcgcagcgg tgtagtgcac cattatcaag acgagtgatt gccacatcgc
     6601 caccggcaaa gatcgcggga tcgcaggtgc ggcaagcctc atcaatgaca atgccattgg
     6661 cagtatcaag gttggcctcg cgagccagtt gctcgttggc gctgatacta ataccgtaaa
     6721 tcaccacatc agcctgaagc gtttccccac tttgcagcgt cagttctact ttttcaccat
     6781 cgaccacatg ttcaatggca ttattgagca gaatgcgcac accagcctgc tgatggcgct
     6841 gtaaaagata gcgttgcacg ggcggtggtg cattacggcc catgacggtt gccgccagtt
     6901 caatcactgt caccttacat ctgcgctgcg ctgcgctgcg tggcgctggc agccagttcc
     6961 agaccaatag ttccggcacc gacaatcacg actgaccgtt cgggctgcag aacttctcgc
     7021 agtctggcgg catcaccggc atggtgtagg gtaaagcagc gttctcccag tgcatcaagc
     7081 aacggcagcg gtcgagctgc cgcgccggtt gctataaaaa gctgatccca gtgccagctt
     7141 tcgccgttgg ttaacactaa ctctcgtgtg tcgcgaccca gcgttttgat ggttacaccg
     7201 gaatgcagat gaacattgtt ttcctgccac cagttagcgg gtaacacctg ctgtaactgc
     7261 ggggaatctt ccagcaacat ggatttcgag agtggaggtc gttcataagg aagatgtcgc
     7321 tcatcggaaa acagatgcag ctcaccggtg aacccttgct ggcgtagcga ggccgcagcc
     7381 attgccgccg cttgcccgcc accgacaata atgatcgttt tttctttcat cgtgcttcct
     7441 taaagatcca gcccagccgc tacgtggcga atgccgcgaa tcgctaaacc cgcatcagcg
     7501 ttgatcatca caccgcttaa tgcgcgatta ttgcgccgcg atgccaacat cacatacggc
     7561 cccgtaaaat ccgccggttg cgggaaaaat tgcagcggta aaatggcggc aattttctcc
     7621 ggcgtcagag actgcattat cgaggtttca ctttgcccga gcgcctgtgg gccgcgcagg
     7681 tcgctggcca taccacacgg gccgacgcca ttcacccgca ctttcggtgc cagttcataa
     7741 gccagttggc gaataagtcc ggttgcggca tgtttactgg tggtgtacag cgggccaccg
     7801 ccgccaggat accaggcggc atttgacagt gtgaaaatca tgctgccttc actggcgatt
     7861 aacgccggag cgcaggcttt tgcgcccagc aggtaaccga gtacgttgac gttaaacagc
     7921 tcgtggaagc cggtttcgag cgtctctgcg ggagtattaa ccagtgaggc attgtgatcc
     7981 cagatgcctg cattgccgat aaaacaatcc agcttgccgg aacgagtcag gatctgatcg
     8041 accgcgcgtt gataatcggc ataacaggtc acgttacctt ccaccgccag aatatgttcg
     8101 ccaaatcgct gacgcagact ggcgactttt gccgccgaca gttccagcgc ggcaacctgc
     8161 gcgccttctt cgataaatcg ctcgaccagc gccagcccta atcccgatcc gccgccggta
     8221 ataaaaatgg actcgttatg cagatcgctc atggctgcgc ctccggtaag tcgatgaaaa
     8281 tgtcaccacc ttcaacgtgt actggataag tggtgagcgg atcggtggcg ggcaggcata
     8341 acgctttccc cgttttcagg caaaaactgg cggcgtgtag cgggcactcc accgtggcgt
     8401 catcttccag atacccttct gacatcgacg cattaccatg gctgcaacga tcgttaatgg
     8461 cataaaactc gccgcccacg ttgaacaagg cgatgacggg cgaggtatct atccggagag
     8521 cctcaccctc cggcacatcc gcaacgggac acgcataaat tcgattcatc agaacagtac
     8581 actcaggtta tgggaagtga tcaccgcttg atccaggacg atatcccgtt ccagcaagcg
     8641 ccagttgtca tcttccagac ggcgaacttt gtcgaaacgc gttccaacat agaatgtttc
     8701 atcgcgctct ttttgtgccc gataaagtag ataattcacc ctcacggcaa atacgtttgg
     8761 gatgtcggtt tcgcttacct ggcagttgct gattaagtga cgagtgcgtg acggcggctc
     8821 ttctgcccag gccatgcccg tttccagacg ggcgattcgc cgctccagct ggtctttggt
     8881 gtcattgaaa atccaggtcg tcggcggctg gacgcctttg cgacggtcgc gtgtttgcgc
     8941 gttaaccgtg gtgcgcatgg tgtaacgaat ctcttcgtcg agctgtgcca accagtcacg
     9001 aaatttccag tcatccagca gactggcttc gtgaaagaga aactggctaa tgcggtgatg
     9061 taactccagt gaaacttgcg cactcatttc atcacctcct gctggtaagc ggcggtttta
     9121 tcgagcactt cctgccagct ttcgctactc agaagatcgg cccagcgttg gtacattcca
     9181 cgagcggcag tttctgaaaa gatatagtta gtaatgccag gaatgccgtc gtcgcggcgc
     9241 ttttcctgac caagccccat ttccagacac agtttgctgt tgcgggcgcg gtggccttta
     9301 agcaatttct ggatttcaca ccagttctcc gagtcatcct gctcgagaaa accagcagga
     9361 ccaaaagcac gagtggcgct gttttcaaaa gcggctttaa cttcatcgga ggcggctttg
     9421 tcagtaatac agaacgccca cacttcaact tgatcagggc cgcgcggatg ccagacgcgg
     9481 agtgtggcag tgccgtttag ccatgaaagc gtggggaata tattgttatg acccgccagg
     9541 cgcagggcgc gaacttcacc taaacgttgt tctgcttcgg cataggtttc gcggtaatag
     9601 cttgaaactg cgccatcgac ccagacatta gcatccggtt tttcagtaaa gaagaaaccg
     9661 ctaccgtgac cgtcctgacc aaattgcagc gcatctttgg cggtttccca caccgggcgg
     9721 gcggtttgtc catcaccgag gcgcttatcg ctgccatcat ctttcgcccc taatacctga
     9781 acggcagaag catggctgaa cagagcatga tactggtcac tggcgaactg ctctgccggg
     9841 aatttccagt tacagttgat cacccacttt tgtacgccgc cgacaatttc ggtgccgcct
     9901 tcgcgacgat ccagcatgcc atccagatac caggcaatgt cacccaggta atcatgcagg
     9961 cccggtgcgc tggtatccca gttgccaaaa atcagtcctt tataactctc cacacaagga
    10021 acttcgttta gcccccagtg ggatttacac aacccttgtg ggtaggcgcg aggttccagc
    10081 ggtacatcga tcaactcgcc gttaatgcca taagaccagc cgtgatacgg gcaggtaaag
    10141 gcgcgagtgt tgccacaatc tgcataactc acacgcatgg cccggtggcg gcattggtta
    10201 agaaacgcct tgatgctgcc gtctttctgg cgcaccacga caactgcatc ttctcccatg
    10261 taggtgttaa agaaatcacc gggttttggg atctggcttt cgtgggcgag aaatagccag
    10321 caacgaccga aaatacgctc aagctccagt tggtaaatgt ccgggtcggt ataaatacgc
    10381 ggagtgaccc gaccgttttg ggtgtcaatc agttggtaaa tgttcaaatc tgagggtgtg
    10441 gtcatatttt ttaatcctga aagataagat gtattgacct tctatttttg tgccataagg
    10501 atgttgctgg ttggttgcta atatgtgaaa tagttaattt ctaccagctg agacgttttt
    10561 tcgataacaa ggggaaggtg atggaactac ggcatttacg ctatttcgtc gcagtggcgc
    10621 aggcactgaa ctttacccgt gcggcggaaa aactgcatac ctcacagcct tcgttaagta
    10681 gccagatccg cgatcttgaa aactgtgtcg gtgttccgtt actggtgagg gataagcgca
    10741 aagtcgcgtt gacggcggcg ggagaatgtt ttctccagga cgcgctggca atcctcgaac
    10801 aagcggaaaa tgccaaatta cgggcgcgga aaattgttca ggaagacaga caattaacca
    10861 ttggctttgt gccatcggcg gaagtgaatt tattgccaaa agtattaccg atgtttcgtc
    10921 tcagacagcc agacaccttg attgagctgg tgagtttaat caccacgcaa caggaggaaa
    10981 aaattcgccg tggtgaactc gacgtcggct taatgcgcca tcctgtttat agcccggaga
    11041 ttgattatct ggagcttttt gacgaaccat tagtggtggt gttaccggtt gatcaccctt
    11101 tagcacatga aaaagagatc accgccgcgc aactggatgg cgtgaatttc gtcagtaccg
    11161 atccggcgta ttccggttcg cttgcgccga tcgttaaagc gtggtttgcg caagaaaaca
    11221 gccagccaaa tatcgtccag gtggcaacga atattctggt gaccatgaat ctggtgggca
    11281 tggggctggg cgtcactttg atacccggtt atatgaataa ttttaatacc gggcaggttg
    11341 tttttcgtcc cattgccgga aatgtgcctt ccattgcttt actgatggcg tggaagaaag
    11401 gggagatgaa gccggcatta cgcgatttca tcgccattgt gcaggaacgt ttggcaagcg
    11461 taacggcata attacgcatt atttttgcaa catgcgccgc ggatcgcagt tttttagtgc
    11521 ttgtgaactg gcgttacaga catcgggaag caggctcgaa tattcttatt tttcaacttg
    11581 caccaacatt cgattaaccc cagactaacg cctcctgacg ggagggactc atggttttgc
    11641 aatccacgcg ctggttggcg ctcggctatt tcacatactt ttttagttac ggcatttttt
    11701 tacctttctg gagcgtctgg cttaaaggga ttggtttaac gccagaaacc atcggcctgt
    11761 tattgggggc aggtctggtt gcccgttttc tcgggagttt gctcatcgcg ccccgcgtca
    11821 gcgatccttc ccgcctgatt tccgccttgc gcgtgctggc actgctgcca cttctctttg
    11881 ctgtcgcctt ctgggcgggg gcgcacgtag cgtggctgat gctggtgatg attggcttta
    11941 acctcttttt ctcaccgctg gtaccgttga ccgatgcact ggcgaatacg tggcaaaagc
    12001 agttcccgct tgattacggc aaagtgcgac tgtggggctc ggtggcgttt gtcattggct
    12061 cggcgctgac gggcaaactg gtcactatgt ttgattatcg ggtgatcctc gtgctgttga
    12121 cgttgggcgt ggcatccatg ctgctcggct ttctcatccg tccgacgatt cagccacaag
    12181 gggcaagccg ccagcaggag agcaccggtt ggtctgcgtg gttggcgctg gttcgccaga
    12241 actggcgctt tctggcctgc gtttgtttat tgcagggggc acatgcggcc tattacggtt
    12301 ttagcgccat ttactggcag gcagctggct actcggcctc ggcggtgggg tatttgtggt
    12361 cgctgggcgt ggtggcggaa gtcattatct ttgcgctgag taataaactt ttccgccgtt
    12421 gtagtgctcg cgatatgctg ttgatctcgg cgatttgcgg cgtagtgcgc tggggcatta
    12481 tgggagcaac tacggcgttg ccgtggttga tagtggtgca aattctgcat tgcggcacct
    12541 tcacggtctg ccacctggcc gccatgcgtt atattgctgc tcgccaggtt agcgaagtca
    12601 tccgtttaca ggcggtttac tctgccgtcg cgatgggcgg cagtatcgct atcatgaccg
    12661 ttttcgccgg tttcctgtat caatatctgg gccacggtgt gttctgggta atggcgctgg
    12721 tggcgcttcc ggcaatgttt ttgcgcccga aagttgttcc ctcatgctga ttcgagcatt
    12781 ttgcggatct gctcctgttg atgtgttgtc agcgtcaggt ccgtatagat cagcggtggt
    12841 gaaaagagcg gtaacggcat ggtataaggc gcaataatca gtgccgcgcc acgcggagcg
    12901 cctgtctgca aaaatgcctt taccgacata tgcttaatgt tgagcggcag taacgttagt
    12961 tcgcgtaact gctgctcaat ctgcgcttct cgctcgctat cattcccggt cagtagaata
    13021 atctgttttt catgcagatc gttttcctgc attagccagg caccgaaaat caccgcgacc
    13081 agaccacttt cctcatcaga aagatggacg ccgtattcac tttcaaatcc ggccagcacc
    13141 gcgcgggtgg tgcgtaccag gcgtggatac agtctggcga actcttccgg cagggtatta
    13201 tcaataccga tggcaaacaa actgcgattt aacgcctggg cgaggtggat ataaagctga
    13261 tcacataacc cctgttcatc ataaaaacga acatttccca gctcacgaaa atggtttacc
    13321 agacgtttga tagactgacg caattgtctg tcccgctgat gcgcatcccg cagtggatcg
    13381 ggaacccgca acatggaaaa aagtaatgcc ataaacagtg gctcatcagg cggtacatgc
    13441 tggagcgccc gacgctgcca gtggcgtcca atttcctgcg ctacctgaaa ttcaaggcag
    13501 gattccgccc agcgacgttg gagcggatta aactgaggcg taattccagc gtggtgctgc
    13561 agcaaacaat attgcagata cagacacagg aaatgaatat cgcgcgattc aaagtgtttt
    13621 tgcagccgtc gggagcagag attcactaac gcttgtagat tggtatcgtc gtaaaagtta
    13681 cgcgcaattc cgcgccgctt cagttcactc ttcagggcgg gggtgaattg gctggtaata
    13741 aatgacggac acagacggaa accacgtcgt agccagtgaa ataaacacaa acgttggtta
    13801 agcactgtac cttcaacccg gtagctaccg tcataaccag tcgtcagtgt gagttgatga
    13861 tagcgcagga tctcccgtcc tgtttcgctg atatcaagac tggcaatatc atcatccaca
    13921 ccattaagct cgctgaaggt tgccatggtg gcggtcaacc ccggctgaaa gagcgtcagc
    13981 aagatctggc agcggcgctg gggagccgaa aggacagatg gtggagcaag cgtaggcatc
    14041 atctgtagat ctcccataag cataattgat aagaatagct aaagtttgct cagggaaatg
    14101 gcgaagggaa tgctaatcat cagaaatgtt ggcaagcgtc acagaatttt tctcatttta
    14161 aactgttctg accagtggat tagcatgtct taaggtggtt ttgtgaatga ttatcgtgct
    14221 agcgatcatg ggttagttct gcggattcaa cgcgctaagg ataaagtcca ccatctgttc
    14281 gcgataaaca ttgccgtgac gatcggaaaa agcatcaatg tgttcgccat ccgggattag
    14341 gatcaaccgt tttggctctt ttgccaggct atacaacttt tcgctgtgct gccatgggat
    14401 aacgtgatcg gctttaccgt gaatgagtaa aagcgggatc gggctgacgc tggcgatgta
    14461 attttcgccg ctgtaactct catcaagtaa gtagccactg ccggggatca tctggttggc
    14521 gatggttgca taagaggcaa atgtggagtc gaggatcacc gcacgtatgc cttcacgatc
    14581 accctgacca ataacagcaa gaatattcgc cccgccaata ctctgcccga acagcaccag
    14641 ccgttgtggg tttacatcac tgcgatggcg caccacattg atggcgcttt gcgtatcgtc
    14701 cagcaatccg gcctgggacg gagtgccttt tgatttacca aacccgcgat aatcaaacat
    14761 aaaaacgttg aaattacgct caggtaacca actgaccagc ggccagtggg cggacatatt
    14821 tccggcattg ccgtgagcat gaatgatggt tgcgatggcg ttgtcagcag ggctcgtcga
    14881 agaaggaata aaccagcctt gcagacgagt accgtcctta gccgtaaatt cgacggattc
    14941 cgccgaccag gggtcgggac cgtaaatttt gtcgtcagga taatagaaga agttgatggc
    15001 aacgcgcagc actaaataaa cgcagaaggc aacaacaaag agaataaaca gaattttggg
    15061 aacgcgtttg ttcactggca gtgccataac ctttccttgc gacttcttta tcatccatta
    15121 atcggggaaa gatggtggga gagtgacgaa agaaagtaaa gcggtgagga agtatttcgg
    15181 aatagcgcct gaaaggcgag ggcgggtgag tgatatcacc cgcctgagtc attaacgctt
    15241 cagagcgtcg cttaactcgt cacgcatgtt cgccagtatg gctttcacaa cacgcgggtt
    15301 accagcaacg atgttaccgg tcagcatata gttatgacca ccggtgaagt cgctgacgat
    15361 gccgcccgct tcacgaacca gcagctcgcc tgccgcgaaa tcccacggac gcagaccgat
    15421 ttcaaagaaa ccgtcaacac gacccgcagc gacgtaagcc agatccagcg ccgcagaacc
    15481 ggtacgacgg aagtctgcac attcgttgaa cagcttgccg acgatgttga tgtaggtagt
    15541 ggcgtactgt tttgctttga acgggaagcc ggtcgccaga atggtaccgt cgagatcgcg
    15601 agcggtgcta ccgcgcagac ggtagccgtt cagctgtgcg ccctgaccgc gagtggcggt
    15661 gaacagttcg ttacgcatag gatcgtatac cacagcaact tcggtgcggc ctttgatacg
    15721 aacggcgata gataccgcga agtgcggcag acgtttgata aagttggtag taccatccag
    15781 tggatcgata acccattgaa catcctgatc agtaccttca agttcaccgc tttcttcggt
    15841 gatgatggtg tgctgtgggt aagatttacg aatcgtgtcg ataatcaccg cttcggcagc
    15901 tttatctacg ttggtcacga aatcgttact gcctttctgg ctcgcttcta cagcgtccgg
    15961 ggtttcatag tttttggcaa ttaaattacc cgccttgcgc gctgcgcgca cggcgatgtt
    16021 cagcatcgga tgcatcggtc tctctcactg gatgttaaag aacgggaaaa cggcgcagag
    16081 tatagcagcg agatcggcat ctgtcttcgt tttatgataa gatgcgtgaa taatctttag
    16141 atgaagaaag acaatgctgc aaaatattcg aattgtgctg gtggagacgt cacacaccgg
    16201 caatatgggt tctgttgccc gtgccatgaa aacaatggga ttaaccaatc tgtggctggt
    16261 taatccactg gtgaaacccg actcccaggc gattgccctg gctgcagggg ccagcgatgt
    16321 gattggtaat gctcacatcg tcgatacgct cgacgaagcg ttagccggtt gtagcctggt
    16381 ggtgggcacc agcgcacgct cccgcacgct gccgtggccg atgctcgacc cgcgcgaatg
    16441 cggtctgaaa agcgtcgctg aagcggcaaa taccccggtg gcgctggtgt ttggtcgtga
    16501 gcgcgtcggc ttgaccaatg aagagttgca gaaatgccat tatcatgtcg cgattgcggc
    16561 aaacccggaa tacagttcgc tgaacctggc aatggcggtt caggttattg cctatgaagt
    16621 gcgtatggcc tggctggcaa ctcaggaaaa tggcgagcag gtcgagcatg aagagacgcc
    16681 gtatccgctg gtcgatgatc tggagcgttt ttatggtcat ctggaacaaa cgctgctggc
    16741 aaccggtttt atccgtgaaa accatccggg gcaggtgatg aataaattgc gccgtctgtt
    16801 tacccgtgcg cgcccggaaa gccaggaatt gaatatcctg cgcgggattc tggcttctat
    16861 tgagcagcag aataaaggta acaaggccga ataacagtgg ttgcctgatg cgacgcttaa
    16921 cgcgtcttat caggcctaca atgtacagaa tcgtaggtcg gataaggtgt tcacaccgca
    16981 tccgacaaca ggtacaaacg ccacgataaa aaaatggcac tgaaggttaa atacccgact
    17041 aaatcagtca agtaaatagt tgaccaattt actcgggaat gtcagacttg accctgctat
    17101 gcaatacccc cacttttaca ataaaaaacc ccgggcaggg gcgagtttga ggtgaagtaa
    17161 gacatgagac tgacatctaa agggcgctat gccgtgaccg caatgcttga cgttgcgctc
    17221 aactctgaag cgggcccggt accgttggct gatatttccg aacgtcaggg aatttccctt
    17281 tcttatctgg aacaactgtt ttcccgtctg cgtaaaaatg gtctggtttc cagcgtacgt
    17341 ggaccaggcg gtggttatct gttaggcaaa gatgccagca gcatcgccgt tggcgaagtg
    17401 attagcgccg ttgacgaatc tgtagatgcc acccgttgtc agggtaaagg cggctgccag
    17461 ggcggcgata aatgcctgac ccacgcgctg tggcgtgatt tgagcgaccg tctcaccggt
    17521 tttctcaaca acattacttt aggcgaactg gttaataacc aggaagtgct ggatgtgtct
    17581 ggtcgtcagc atactcacga cgcgccacgc acccgcacac aagacgcgat cgacgttaag
    17641 ttacgcgctt aataaaaaga attcagaatc aggccggagt gctaaatact ccgtaaacac
    17701 ggtcgtacat ccagccggta gcctgattcc ttgcattgag tgatgtacgg agtttataga
    17761 gcaatgaaat taccgattta tctcgactac tccgcaacca cgccggtgga cccgcgtgtt
    17821 gccgagaaaa tgatgcagtt catgacgatg gacggaacct ttggtaaccc ggcctctcgt
    17881 tctcaccgtt tcggctggca ggctgaagaa gcggtagata tcgcccgtaa tcagattgcc
    17941 gatctggtcg gcgctgatcc gcgtgaaatc gtctttacct ctggtgcaac cgaatctgac
    18001 aacctggcga tcaaaggtgc agccaacttt tatcagaaaa aaggcaagca catcatcacc
    18061 agcaaaaccg aacacaaagc ggtactggat acttgccgtc agctggagcg cgaaggtttc
    18121 gaagtcacct acctggcacc gcagcgtaac ggtattatcg acctgaaaga actcgaagcg
    18181 gcgatgcgtg acgacaccat ccttgtttcc atcatgcacg taaataacga aatcggcgtg
    18241 gtgcaggata tcgcggctat cggcgaaatg tgccgtgctc gtggcattat ctatcacgtt
    18301 gatgcaaccc agagcgtggg caaactgcca atcgacctga gccagttgaa agttgacctg
    18361 atgtctttct ccggtcacaa aatctatggc ccgaaaggta tcggtgcgct gtatgtgcgt
    18421 cgtaaaccgc gtgtacgcat cgaagcgcaa atgcacggcg gcggtcacga gcgcggtatg
    18481 cgttccggca ctctgcctgt tcaccagatc gtcggaatgg gcgaagccta tcgcatcgca
    18541 aaagaagaga tggcgaccga gatggaacgt ctgcgcggcc tgcgtaaccg tctgtggaac
    18601 ggcatcaaag atatcgaaga agtttacctg aacggtgacc tggaacacgg tgcgccgaac
    18661 attctcaacg tcagcttcaa ctacgttgaa ggtgagtcgc tgattatggc gctgaaagac
    18721 ctcgcagttt cttcaggttc cgcctgtacg tcagcaagcc tcgaaccgtc ctacgtgctg
    18781 cgcgcgctgg ggctgaacga cgagctggca catagctcta tccgtttctc tttaggtcgt
    18841 tttactactg aagaagagat cgactacacc atcgagttag ttcgtaaatc catcggtcgt
    18901 ctgcgtgacc tttctccgct gtgggaaatg tacaagcagg gcgtggatct gaacagcatc
    18961 gaatgggctc atcattaatc ggtatcggaa tcaggagaat ttataatggc ttacagcgaa
    19021 aaagttatcg accattacga gaatccgcgt aacgtgggtt cctttgataa caacgacgag
    19081 aacgtcggca gcggcatggt gggtgcaccg gcctgtggcg acgtaatgaa gttgcagatt
    19141 aaagtcaacg atgaaggtat cattgaagac gcgcgtttta aaacttacgg ctgcggttcc
    19201 gctatcgctt ccagctccct ggtcaccgaa tgggtgaaag ggaagtctct cgacgaagcg
    19261 caggcgatca aaaacaccga tattgctgaa gaacttgaac tgccgccggt gaaaattcac
    19321 tgttctattc tggcagaaga cgcgatcaaa gccgccattg cggactataa aagcaaacgt
    19381 gaagcaaaat aagagttgag gtttggttat gtcgattaca ctgagcgaca gtgcagcagc
    19441 gcgagtaaat acctttctgg ctaaccgcgg caaagggttt ggcctgcgtc tgggcgtgag
    19501 aacctccgga tgttcaggta tggcttatgt actggaattt gttgacgaac cgacgccgga
    19561 agacatcgtg tttgaagaca aaggcgtgaa agtcgtggtc gatggcaaaa gcctgcaatt
    19621 tctggacggt acgcagctgg acttcgtaaa agaaggcctg aatgaagggt ttaaattcac
    19681 caacccgaac gttaaagatg agtgtggttg cggcgaaagc ttccacgttt gatgcgcata
    19741 ccgataaccc caccgtggtc gcctgcgcgt ggggtttgtt ttacctgatt cgccgttatc
    19801 gcggcggatc gcagccctga gaatgttatg gattacttca ccctctttgg cttgcctgcc
    19861 cgttatcaac tcgataccca ggcactgagc ctgcgttttc aggatctaca acgtcagtat
    19921 catcctgata aattcgccag cggaagcctg gcggaacaac tcgccgccgt acagcaatct
    19981 gcaaccatta accaggcctg gcaaacgctg cgtcatccgt taatgcgcgc ggaatatttg
    20041 ctttctttac acggttttga tctcgccagc gagcagcata ctgtgcgcga caccgcgttc
    20101 ctgatggaac agttggagct gcgcgaagag ctggacgaga tcgaacaggc gaaagatgaa
    20161 gcgcggctgg aaagctttat caaacgtgtg aaaaagatgt ttgatacccg ccatcagttg
    20221 atggttgaac agttagacaa cgaggcgtgg gacgcggcgg cggatacggt gcgtaagctg
    20281 cgttttctcg ataaactgcg gagcagtgcc gaacaactcg aagaaaaact gctcgatttt
    20341 taatttctgg aagctaaaca tggccttatt acaaattagt gaacctggtt tgagtgctgc
    20401 gccgcatcag cgtcgtctgg cggctggtat tgacctgggc acaaccaact cgctggtggc
    20461 gacagtgcgc agcggtcagg ccgaaacgtt agccgaccat gaaggccgtc acctgctgcc
    20521 atctgttgtt cactatcaac agcaagggca ttcggtgggt tatgacgcgc gtactaacgc
    20581 agcgcttgac accgccaaca ccattagttc tgttaaacgc ctgatggggc gctcgctggc
    20641 tgatatccag caacgctatc cgcatctgcc ttatcaattc caggccagcg aaaacggcct
    20701 gccgatgatt gaaacggcgg cggggctact gaacccggtg cgcgtttctg cggacatcct
    20761 caaagcactg gcggcgcggg caactgaagc cctggcaggc gagctggatg gtgtagttat
    20821 caccgttccg gcgtactttg acgatgccca gcgtcagggc accaaagacg cggcgcgtct
    20881 ggcggggttg catgttctgc gcttacttaa cgaaccgacc gctgcggcta tcgcctacgg
    20941 gctggattcc ggtcaggaag gcgtgatcgc tgtttatgac ctcggtggcg ggacgtttga
    21001 tatttccatt ctgcgcttaa gtcgcggcgt gtttgaagtg ctggcaaccg gcggtgattc
    21061 cgcgctcggc ggcgatgatt tcgaccatct gctggcggat tacattcgcg agcaggcgga
    21121 cattcctgat cgtagcgata accgcgttca gcgtgaactg ctggatgccg ccattgcagc
    21181 caaaatcgcg ctgagcgatg cggactccgt gaccgttaac gttgcgggct ggcagggcga
    21241 aatcagccgt gaacaattca atgaactgat cgcgccactg gtaaaacgaa ccttactggc
    21301 ttgtcgtcgc gcgctgaaag acgcgggtgt agaagctgac gaagtgctgg aagtggtgat
    21361 ggtgggcggt tctactcgcg tgccgctggt gcgtgaacgg gtaggcgaat ttttcggtcg
    21421 tccaccgctg acttccatcg acccggataa agtcgtcgct attggcgcgg cgattcaggc
    21481 ggatattctg gtgggtaaca agccagacag cgaaatgctg ctgcttgatg tgatcccact
    21541 gtcgctgggc ctcgaaacga tgggcggcct ggtggagaaa gtgattccgc gtaataccac
    21601 tattccggtg gcccgcgctc aggatttcac cacctttaaa gatggtcaga cggcgatgtc
    21661 tatccatgta atgcagggtg agcgcgaact ggtgcaggac tgccgctcac tggcgcgttt
    21721 tgcgctgcgt ggtattccgg cgctaccggc tggcggtgcg catattcgcg tgacgttcca
    21781 ggtcgatgcc gacggtcttt tgagcgtgac ggcgatggag aaatccaccg gcgttgaggc
    21841 gtctattcag gtcaaaccgt cttacggtct gaccgatagc gaaatcgctt cgatgatcaa
    21901 agactcaatg agctatgccg agcaggacgt aaaagcccgt atgctggcag aacaaaaagt
    21961 agaagcggcg cgtgtgctgg aaagtctgca cggcgcgctg gctgctgatg ccgcgctgtt
    22021 aagcgccgca gagcgtcagg tcattgacaa tgctgccgct cacctgagtg aagtggcgca
    22081 gggcgatgat gttgacgcca tcgaacaagc gattaaaaac gtagacaaac aaacccagga
    22141 tttcgccgct cgccgcatgg accagtcggt tcgtcgtgcg ctgaaaggcc attccgtgga
    22201 cgaggtttaa tatgccaaag attgttattt tgcctcatca ggacctctgc cctgatggcg
    22261 ctgttctgga agctaatagc ggtgaaacca ttctcgacgc agcgctgcgt aacggtatcg
    22321 agattgaaca cgcctgtgaa aaatcctgtg cttgcaccac ctgccactgc atcgttcgtg
    22381 aaggttttga ctcactgccg gaaagctcag agcaggaaga cgacatgctg gacaaagcct
    22441 ggggactgga gccggaaagc cgtttaagct gccaggcgcg cgtcaccgac gaagatttag
    22501 tggtcgaaat cccgcgttac actatcaacc atgcgcgtga gcattaacag aggttagtat
    22561 gggacttaag tggaccgata gccgcgaaat tggcgaagca ctgtacgatg cgtatcccga
    22621 tcttgatccg aaaacggttc gattcaccga tatgcatcag tggatttgcg atctggaaga
    22681 tttcgacgac gacccgcagg catccaacga gaaaatcctc gaagcgattt tgttagtctg
    22741 gctggacgaa gccgagtgaa tgaacgggct gcctgggggt agcccgtttt gctgttaaac
    22801 gaataaggat aactaaaatg acagaagcga tgaagattac catctctacc caacctgccg
    22861 acgcgcgctg gggagaaaaa gcaacttaca gcattaataa tgacggcatt accctgcatt
    22921 tgaacggggc agacgatctg gggctgatcc agcgtgcggc ccgcaagatt gacggtctgg
    22981 gcatcaagca tgttcagtta agcggtgaag gctgggatgc ggatcgctgc tgggcattct
    23041 ggcaaggtta taaaggcccg aaaggcacgc gtaaagtgga gtggccggat ctggacgatg
    23101 cccagcgcca ggaactggat aaccgcctga tgatcatcga ctgggtgcgt gacaccatca
    23161 acgcaccggc agaagaattg ggaccatcgc aactggcaca gcgtgctgtt gatctgatca
    23221 gcaacgtcgc gggcgatcgt gtgacttatc ggatcaccaa aggcgaagat ctgcgtgagc
    23281 aaggttatat ggggctgcac actgtcgggc gcggttcaga acgttctccg gtattgctgg
    23341 cgctggatta caacccaact ggcgataaag aagcaccggt gtacgcgtgc ctggtaggta
    23401 aaggtatcac ttttgactcc ggcggctaca gcatcaaaca gactgcgttt atggactcga
    23461 tgaagtcgga catgggcggc gcggcaacgg ttaccggggc gctggcattt gccattacgc
    23521 gcggactgaa caagcgcgtg aagctgttcc tctgctgtgc ggataacctg attagcggca
    23581 atgcgttcaa gctgggcgat atcatcacct atcgcaacgg taaaaaagtt gaagtgatga
    23641 acactgatgc cgaagggcgt ctggtgcttg ccgatggtct gattgatgcc agtgcgcaga
    23701 aaccggaaat gatcattgat gcggcgaccc tcaccggggc ggcgaaaact gcgctgggta
    23761 atgattatca cgcgctgttc agttttgacg atgcgcttgc cggtcgttta ctggcgagtg
    23821 cctcacaaga gaacgaacca ttctggcgtc tgccgctggc ggaattccac cgcagccagc
    23881 tgccgtctaa ctttgccgaa ctgaacaata ccggaagcgc ggcgtatccg gcaggcgcga
    23941 gcacggcggc gggcttcctg tcgcactttg ttgagaacta tcagcaaggc tggctgcata
    24001 tcgactgctc ggcgacttac cgtaaagcgc cggttgaaca gtggtctgcg ggcgctacgg
    24061 gacttggtgt gcgcacgata gctaatctgt taacggcgta aactaatatg ccggatgcgg
    24121 cgtaaacgcc ttatccggcc tacaaattct gcaaattcaa aaaattgcga atcccttgta
    24181 ggcctgataa gcgtagcgca tcaggcattt gcgccccgaa cgttgaatga attgtggttc
    24241 ttatgtccga aacaaaaaac gaacttgaag acctgctgga aaaagcagca accgaaccgg
    24301 cgcaccgccc ggcctttttc cgtactctac tggaatccac cgtctgggtg cctggtacgg
    24361 cggcgcaggg cgaggctgtg gttgaagata gtgcgcttga tttacagcac tgggaaaaag
    24421 aagacggcac cagcgtcatt ccttttttca cctcgttaga agcgcttcaa caggcggttg
    24481 aagacgaaca ggcatttgtc gtaatgcccg ttcgcacatt gtttgagatg acccttggcg
    24541 aaacgctctt ccttaatgcc aaactgccga ccggtaaaga atttatgccg cgtgaaatca
    24601 gtttgttgat tggtgaagag ggaaatccgc tgagcagcca ggaaatcctg gaaggcggtg
    24661 aatcgctgat attatcggaa gtcgcagagc cgccagcaca aatgattgat tcactcacca
    24721 ccttatttaa aaccattaag ccggtgaagc gtgcttttat ttgttcaatt aaagagaacg
    24781 aagaggcaca gcctaattta cttattggca ttgaagccga tggtgatatc gaagaaatta
    24841 ttcaggcgac gggaagtgta gcgaccgata cattacctgg cgatgaacca atcgatattt
    24901 gtcaggtgaa aaaaggggaa aaaggaatta gccactttat taccgaacat attgcgccat
    24961 tctatgaacg tcgctggggt ggttttttgc gtgactttaa acaaaaccgg ataatctaaa
    25021 atatgccgcc ctaaagggcg gcattcgggc ttacttgctg ctctgaaggt tgttaaccac
    25081 cagtaaattg tcgccggaga gactccggtt attatttact tgggaaaaca gcgcaccaga
    25141 aacaataagt acgatacaga agcaccgtat cttcattgtg ctgtcgtgat gcgaagaccg
    25201 catcacgaca gcaccactgg cgatattgcc gcgatagcaa gcgctgagac gcgcttgctc
    25261 taacggtcgc ggaacatcct ttccttaagc cagcgcccgc ttgcgagacg gcggaaaggg
    25321 ccgcagtgga taatacacta acagaacaat aataactctc ttttgcttga caaaaagaga
    25381 gttactggcg agtattgtct ccgctgagac gcgcttgcat tactacggta atacaacctg
    25441 ctggaaaggc cgcgaaccgg accagcaaca aataaccgcc tgattttggc ggttttttgt
    25501 tttttaaagg aaagtaaatt tacatgttta agtaatatct tttaccggtt agaattttta
    25561 attttgagca ggatttgttt atatttgtct gaattcggac taataagcca ggttatttta
    25621 ttaccgaaca cggcggaaag ggccggtgtc gataatacgc taaataaaca ataattactc
    25681 tcttttgctt gacaaaaaga gagttactgg tgagtattgt tttactgtga agcacctgcg
    25741 ttgctcatgt aacacaagtc aacctgctgg aaaggccgcg aaccagacca gcaataaaaa
    25801 aacgccaaat tcggcggttt tttattgcct gtaaaatact tatttcactg gctcaaccgg
    25861 taaatctgcc cgcgcgcccc attcactcca tgcgccgtcg tacagtttca cgtttggcac
    25921 atccagcgtc gcgagtgcta acaaaaccac ggctgccgtt acaccagagc cgcagctgac
    25981 gataatcggt ttgtcatagc tgacgccgcg accaaaaaat atcgcatcca gttcatcggt
    26041 cgtttttagt tcgccttcgc gcaccagttc cgtccacgga acgttcaatg caccgggaat
    26101 atgtccgcga cgtaaacctg ggcgaggttc atcaacttct gcgttaaaac gtgcagccgg
    26161 gcgggcatca ataatttgcg ccgtattttc atggcttgcc aataacacat cggttacttt
    26221 caccactgct tcaggattaa acgcggcgtt aaactctcct tccggcagct ctactgcacc
    26281 ttcttctaat agcaggtcat cgcgctgcca gcctgcaagt ccccgttact ttattcgtac
    26341 cccttataat ggggtgttag ccagccagac ccggcatgat tactgccccc agtcgtccat
    26401 gatccggggg g
//
LOCUS       DAETPZ010000052        26155 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.052, whole genome shotgun sequence.
ACCESSION   DAETPZ010000052 DAETPZ010000000
VERSION     DAETPZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.052"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..746
                     /locus_tag="K0W59_002468"
     CDS             <1..746
                     /locus_tag="K0W59_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3023470.1"
                     /translation="VDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWN
                     NVTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIAT
                     LGSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKL
                     SFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPL
                     GLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1080..3359
                     /gene="ydeP"
                     /locus_tag="K0W59_002469"
     CDS             1080..3359
                     /gene="ydeP"
                     /locus_tag="K0W59_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HBI3023471.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3607..3804
                     /gene="safA"
                     /locus_tag="K0W59_002470"
     CDS             3607..3804
                     /gene="safA"
                     /locus_tag="K0W59_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HBI3023472.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQD"
     gene            3878..4639
                     /gene="ydeO"
                     /locus_tag="K0W59_002471"
     CDS             3878..4639
                     /gene="ydeO"
                     /locus_tag="K0W59_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HBI3023473.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            5041..6724
                     /locus_tag="K0W59_002472"
                     /pseudo
     CDS             5041..6724
                     /locus_tag="K0W59_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
     gene            6776..7931
                     /locus_tag="K0W59_002473"
                     /pseudo
     CDS             6776..7931
                     /locus_tag="K0W59_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
     gene            complement(7897..8019)
                     /locus_tag="K0W59_002474"
     CDS             complement(7897..8019)
                     /locus_tag="K0W59_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023474.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8222..9907
                     /locus_tag="K0W59_002475"
                     /pseudo
     CDS             8222..9907
                     /locus_tag="K0W59_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            9945..12317
                     /locus_tag="K0W59_002476"
     CDS             9945..12317
                     /locus_tag="K0W59_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBI3023475.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            12374..15157
                     /locus_tag="K0W59_002477"
     CDS             12374..15157
                     /locus_tag="K0W59_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBI3023476.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALQQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGAKTWPGNKVIETFESMG
                     LRFGLDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     SEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKFPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTIENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            15519..16919
                     /locus_tag="K0W59_002478"
     CDS             15519..16919
                     /locus_tag="K0W59_002478"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBI3023477.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            17075..18610
                     /gene="gadC"
                     /locus_tag="K0W59_002479"
     CDS             17075..18610
                     /gene="gadC"
                     /locus_tag="K0W59_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HBI3023478.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            18741..20060
                     /locus_tag="K0W59_002480"
     CDS             18741..20060
                     /locus_tag="K0W59_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HBI3023479.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            20346..20387
                     /locus_tag="K0W59_002481"
     CDS             20346..20387
                     /locus_tag="K0W59_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023480.1"
                     /translation="MVQLVDLARCVSF"
     gene            20425..21570
                     /gene="dosC"
                     /locus_tag="K0W59_002482"
     CDS             20425..21570
                     /gene="dosC"
                     /locus_tag="K0W59_002482"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HBI3023481.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            21495..22112
                     /locus_tag="K0W59_002483"
                     /pseudo
     CDS             21495..22112
                     /locus_tag="K0W59_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            22370..22702
                     /gene="ddpX"
                     /locus_tag="K0W59_002484"
                     /pseudo
     CDS             22370..22702
                     /gene="ddpX"
                     /locus_tag="K0W59_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            22716..23612
                     /locus_tag="K0W59_002485"
     CDS             22716..23612
                     /locus_tag="K0W59_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HBI3023482.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            23609..24595
                     /locus_tag="K0W59_002486"
     CDS             23609..24595
                     /locus_tag="K0W59_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI3023483.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            24588..25514
                     /locus_tag="K0W59_002487"
                     /pseudo
     CDS             24588..25514
                     /locus_tag="K0W59_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(25570..26001)
                     /gene="osmC"
                     /locus_tag="K0W59_002488"
     CDS             complement(25570..26001)
                     /gene="osmC"
                     /locus_tag="K0W59_002488"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HBI3023484.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         7121 a         6009 c         6085 g         6940 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgcgcacaat tgaagccaga gtcttatttt ttatattgaa aaatatgtta atttaaacac
      901 tatgagagat gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa
      961 aatagaaaga gagtagtccc ctactcttat atatccatgt tggcgataat ccctttgtat
     1021 gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga
     1081 tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg
     1141 tagcgaatgc agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca
     1201 tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct aagcacagtg
     1261 cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ctgggaagtc acggataagc
     1321 aggtaaatgc ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc
     1381 acgagcttga ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact
     1441 gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct
     1501 atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct
     1561 ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca
     1621 tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg
     1681 tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta
     1741 caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga
     1801 tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt
     1861 ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg
     1921 gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg
     1981 caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg
     2041 gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt
     2101 ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca
     2161 ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc
     2221 aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc
     2281 cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt
     2341 ctgcagagtt tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg
     2401 gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct
     2461 gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa
     2521 cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg
     2581 cacggcatag ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg
     2641 gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt
     2701 taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg
     2761 cagcactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc
     2821 gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc
     2881 ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta
     2941 aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta
     3001 agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg
     3061 atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag
     3121 ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg
     3181 gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc
     3241 gctcactggt gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc
     3301 cattaagtgg cattcctggc tataaaagta ttccggttga attagaacca tcaaattaat
     3361 gcctcttctc atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta
     3421 ttgcgcaatt tactcacatg gattaaatta aactacatac tataagatat aaacttctgc
     3481 ctacagctgt aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc
     3541 agcctgttat attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata
     3601 tttgaaatgc atgcgaccac agtgaaaaac aaaatcacgc aaagagataa ctataaagaa
     3661 atcatgtctg caattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg
     3721 gcaattgatc agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc
     3781 attatcaata gtttgcaaga ctgaaaataa acagcaaatt ataaatgtga aatgtttaaa
     3841 aggtatcgat aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat
     3901 atttattcat catgctttca acgctaacat tttagataaa gattacgcct tctctgacgg
     3961 cgagatcctg atggtagata acgctgttcg tacacatttt gaaccttatg agcggcattt
     4021 taaagaaatc ggatttaatg aaaataccat taaaaaatat ctacaatgca ctaacatcca
     4081 gacagtgacg gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt
     4141 gcttaatgaa atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga
     4201 acttttgctt ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt
     4261 aactaacggt gtgctatccg tttccgggaa agtgagaaat attgtcaaca tgaagctggc
     4321 gcacccatgg aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa
     4381 gaaaaaacta aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca
     4441 gcacgcaaaa aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg
     4501 ttatgccagt acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa
     4561 gagagtttct aaggagtacc gttgtcaaag gcacacgggt atgaatacgg gcaacacgat
     4621 gagtgcttta gctatttgat tatttgctaa cgagtagtca accacgcacg ctacgtaaga
     4681 attatatggg gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat
     4741 aaataagtga catccatcac atatttatgc acttgcataa cctgttgcat gattatttat
     4801 aatctcaatt cggcattttg tcagtaaaat gcaataattt attaaatatc aataaattag
     4861 ttgtttatcg gcgagaaatt acttaataga acagaaagta atgtcaacgc tttatggact
     4921 gttatttccc tttttttagc taaatctgct atctctttat gtgactaact tcacttacat
     4981 ccacttattt ctcttcgtaa aattacttcg gaattaagta caataagaag aggaacattt
     5041 atgaagtctg cattaaagaa aagtgtcgta agtacctcga tatctttgat actggcatct
     5101 ggtatggctg catttgctgc tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca
     5161 aacgttgctt tctcagactt tacgccgact gaatacagta ccaaaggaaa gccaaatatt
     5221 atcgtactaa ccatggatga tcttggttat ggacaacttc cttttgataa gggatctttt
     5281 gacccaaaaa caatggaaaa tcgtgaagtt gtcgatacct acaaaatagg gatagataaa
     5341 gccattgaag ctgcacaaaa atcaacgccg acgctccttt cattaatgga tgaaggcgta
     5401 cgttttacta acggctatgt ggcacacggt gtttccggcc cctcccgtgc cgcaataatg
     5461 accggtcgag ctcccgcccg cgttggtgtc tattccaata ccgatgctca ggatggtatt
     5521 ccgctaacag aaactttctt gcctgaatta ttccagaatc atggttatta cactgcagca
     5581 gtaggtaaat ggcacttgtc aaaaatcagt aatgtgccgg taccggaaga taaacaaacg
     5641 cgtgactatc atgacaactt caccacattt tctgcggaag aatggcaacc tcaaaaccgt
     5701 ggctttgatt actttatggg attccacgct gcaggaacgg catattacaa ctccccttca
     5761 ctgttcaaaa atcgtgaacg tgtccccgca aaaggttata tcagcgatca gttaaccgat
     5821 gaggcaattg gcgttgttga tcgtgccaaa acacttgacc agccttttat gctttacctg
     5881 gcttataatg ctccgcacct accaaatgat aatcctgcac cggatcaata tcagaagcaa
     5941 tttaataccg gtagtcaaac agcagataac tactacgctt ccgtttattc tgttgatcag
     6001 ggtgtaaaac gcattctcga acaactgaag aaaaacggac agtatgacaa tacaattatt
     6061 ctctttacct ccgataatgg tgcggttatc gatggtcctc tgccgctgaa cggggcgcaa
     6121 aaaggctata agagtcagac ctatcctggc ggtactcacc accccaatgt ttatgtggtg
     6181 gaaaggaaaa cttcaacccg gtaattatga caagctgatt tccgcaatgg atttctaccc
     6241 aacagctctt gatgcagccg atatcagcat tccaaaagac cttaagctgg atggtgtttc
     6301 cttgctgccc tggttgcaag ataagaaaca aggcgagcca cataaaaatc tgacctggat
     6361 aacctcttat tctcactggt ttgatgagga aaatattcca ttctgggata attaccacaa
     6421 atttgttcgc catcagtcag acgattaccc gcataacccc aacactgagg acttaagcca
     6481 attctcttat acggtgagaa ataacgatta ttcgcttgtc tatacagtag aaaacaatca
     6541 gttaggtctc tacaaactga cggatctaca gcaaaaagat aaccttgccg ccgccaatcc
     6601 gcaggtcgtt aaagagatgc aaggcgtggt aagagagttt atcgacagca gccagccacc
     6661 gcttagcgag gtaaatcagg agaagtttaa caatatcaag aaagcactaa gcgaagcgaa
     6721 ataactaaac cttcatgcgg cggatttttc cgccgcctta ttgagcgaga tagcaatgca
     6781 cgttacagcc aagccctcca gttttcaatg taatctcaaa tgtgattact gtttttacct
     6841 tgaaaaagag tcgcagttta ctcatgaaaa atggatggat gacagcactc tgaaagagtt
     6901 catcaaacaa tatatcgaag cgtctggcaa tcaggtctat tttacctggc aaggcggtga
     6961 acccactctg gctggcctgg attttttccg taaagttatt cactatcaac aacgctatgc
     7021 aggccaaaaa cgtattttta atgcattaca aacgaatggc attttattga ataatgaatg
     7081 gtgtgccttt ctcaaagaac atgattttct ggttggtatc tcgatcgatg gcccccagga
     7141 gttacatgac cgttacagac gcagtaattc aggtaacggt acttttgcaa aagtgatagc
     7201 agccatcgag cgtctgaaat catatcaagt agagtttaat acgttaaccg tcattaataa
     7261 cgttaatgtc cattaccctc ttgaggttta tcatttttta aaatctatcg gcagtaaaca
     7321 tatgcaattt atcgaattgc tagaaaccgg gacgccgaat attgatttca gtggtcatag
     7381 tgagaacaca ttccgtatca ttgatttttc tgtgcctccc acggcttatg gcaagtttat
     7441 gtcaaccatt tttatgcaat gggttaaaaa cgatgtgggt gaaattttca ttcgtcagtt
     7501 tgaaagcttt gtcagccgtt ttttggggaa tgggcataca agttgtattt tccaggagtc
     7561 ctgcaaggat aatctggttg ttgaaagtaa tggagacatt tacgaatgcg accattttgt
     7621 ctatccacag tacaaaattg gaaacattaa taaatctgaa ctcaaaacga tgaacagtgt
     7681 acaactgaca gcgcaaaaaa cggatttcag cgaaatgtca gcaatgtgca tataaaccta
     7741 tctgcaatgg cggttgtcct aagcatcgta ttactaaagt aaacaatgag actgtttctt
     7801 atttttgcga aggttataaa atcctttttt caaccatggt accttatatg aacgccatgg
     7861 ttgagttagc taagaacaga gtaccgcttt accacattat ggatgttgca aaacaaatgg
     7921 agaataatta ataacgctct cttttaactc cattcttagc ctccttctta tgaccttaac
     7981 aaggcacccg acataaattc ctgtcgggtg tttcgtcatc atcatcttcc cttgcgaaaa
     8041 tcctggctta cgtggtatgc gtcagggcat tagcaatacc cttgattatc aatagcataa
     8101 ccataaccac tgaaaattaa aaatttctat atatttcata ttgttaaatg atacttaaat
     8161 aaatttcatc agtaaatcat tgccatagat aatcattgga tggcacaatc aattttcgct
     8221 aatgataacc attcccatta cgttttgtat gttgatagca aagtatctct gtctgctgaa
     8281 gcccttttgg ctgcgtaaga ataataaaac ttcagtgttg ttaatcatta ttatcctcgc
     8341 aatgatcctt ggcgtggtta aaattcaggt ctggttaaac gactggaata atgatttttt
     8401 caatgcgcta agccagaaag aaaccgataa gctttggcag cttgttcttt ggttccctgc
     8461 cctgctgggg atctttgtat taatatccgt aaacaaaaca tggttaatta aactactgac
     8521 tattcgctgg cgcgaatggt taacagatta ctaccttaat cgctggtttg cagataaaaa
     8581 ctactacttc acgcagatct acggcgagca taaaaataca gataaccctg accaacgtat
     8641 cgctgaagac attctcttac tgatcaacaa aacgctcagt ctttcatttg gcttcatcca
     8701 gtcacttagc atgctgatca cctttaccgt tattctttgg caaagcgcgg gtacgctctc
     8761 attcactgtt ggtggaacag aatggaatat ccaggggtat atggtctata ccgtcgtgct
     8821 cattgtgatc ggtggaactt tatttaccca taaggtgggt aaacgtattc gtccgcttaa
     8881 tgtggaaaaa caacgtagcg aagcgacatt tcgaactaat cttgtgcagc ataataagca
     8941 ggcagaactg attgcgctaa gcaatgctga gtctctacaa cgccaggagt tgcgcgacaa
     9001 ttttcatacc attaaagaga actggcatcg tttaatgaat cgtcagcggt ggcttgatta
     9061 ctggcaaaat atttattcgc gctcgcttag cgttcttccc tactttctgt tattgccgca
     9121 gtttattagc gggcagatca atctgggcgg actgatgaaa tcgcgccagg catttatgct
     9181 agtatcgaac aatttaagct ggtttattta taaatatgac gaacttgctg aactggctgc
     9241 ggttatcgat cgcttgtatg agttccatca actcactgaa tagcgcccta cgaataagcc
     9301 taaaaattgc caacatgcgg tacaagtggc taatgcgagt attcgtacgc ctgataataa
     9361 gatcatatta gagaacttga actttcatgt ttcgccaggc aaatggctat tactgaaagg
     9421 ctactctggt gcgggaaaaa ccacactgct taaaacatta tcccactgct ggccgtgatt
     9481 taaaggtgat atttcttctc ctgctgacag ttggtatgtg tcacaaacac cgttaatcaa
     9541 aagcggctta ctgaaagaga ttatttgtaa agcacttccc ctgcccgtag acgataaatc
     9601 gttgagcgaa gtactgcatc aggttggtct ggggaaattg gctgcgcgta ttcatgacca
     9661 cgatcgctgg ggagatattc tttccagcgg cgaaaaacaa cgtatcgccc tggcacgatt
     9721 aattttacga cgtccgaaat ggatatttct tgacgaaact acctctcatc ttgaggaaca
     9781 agaggctata cgcttactgc gtttagtgcg tgaaaaacta cccacaagcg gcgtcattat
     9841 ggttacacat caacccggtg tctggaacct ggctgatgat atttgtgaca ttagcacggt
     9901 tttataacaa caataaaagc cctgctgtac ggagatataa ataaatgaag cgagttctta
     9961 ttcctggcgt cattttatgt ggcgctgatg tggcgcaggc cgtcgatgac aaaaacatgt
    10021 acatgcattt ttttgaagag atgacggtct atgctcctgt ccctgtaccc gtaaacggca
    10081 acacgcatta caccagtgaa agcatcgagc gtttaccgac cgggaatggc aatatcagcg
    10141 atctgctgag aaccaaccct gcggtacgca tggattcaac gcaaagtacc tcgttgaacc
    10201 agggagatat tcgccctgag aaaatctcta ttcacggtgc gtcgccctac cagaatgcct
    10261 atttgattga cggtattagt gcaactaata acctgaaccc agcgaatgag tccgatgcca
    10321 gtagtgcaac caatattagc gggatgtcac aggggtatta tcttgatgtc agcttactgg
    10381 acaatgtgac gctttatgac agttttgtgc cggttgaatt tggtcgcttc aatggcgggg
    10441 taattgatgc aaagatcaaa cgcttcaacg ctgatgatag caaggtgaaa ttgggttatc
    10501 gcactacgcg tttggactgg ttaacatcgc atatcgatga gaataacaag agcgcattta
    10561 atcaaggttc ttcaggaagt acctatttct ctccagattt taaaaagaac ttttatacct
    10621 tgtcgtttaa tcaggagctg gctgataact ttggcgttac cgccggttta tcgcgccgcc
    10681 agtctgatat cacccgcgcg gattatgttt cgaatgacgg cattgtcgcc ggtcgggcac
    10741 agtataaaaa cgttatcgat actgcattga gcaaatttac ctggtttgcc agcgaccgct
    10801 ttacccacga tttaacctta aaatataccg gctccagccg tgattataat accagcacct
    10861 tcccgcaatc tgatcgcgaa atgggtaata aatcctatgg tctggcatgg gatatggata
    10921 cgcaactcgc atgggccaaa ctacgtacca ccgttggttg ggatcatatt agtgattata
    10981 cccgtcacga tcatgacatc tggtacaccg aactttcatg tacatatggt gatattacag
    11041 ggcgttgtac ccgtggcgga ttaggacaca tttcccaggc tgtagataat tacaccttca
    11101 aaacacgcct ggactggcaa aaattcgccg tgggtgatgt ttcgcatcaa ccctacttcg
    11161 gcgcggaata catctattcc gatgcatgga ctgaacgcca taaccagtct gaatcctatg
    11221 tgattaatgc tgcaggaaag aaaactaacc ataccattta ccataaaggt aaaggcagcc
    11281 tgggaattga caactacacg ctgtatatgg cggatcacat tagctggcgg aatgtgtcgt
    11341 taatgcccgg tgtgcgttat gactatgaca actatctgtc aaaccacaat atctccccgc
    11401 gctttatgac ggaatgggat atttttgctg atcaaacctc aatgattacc gccggttata
    11461 accgttacta tggcgggaat attcttgata tgggattacg tgatatccgc aatagctgga
    11521 cggaatcggt atcaggtaat aaaaccctga cgcgttatca gaatttgaaa acgccttata
    11581 acgatgaact ggcaatggga ttgcagcaaa aaatcgataa gaacgttatt gcacgcgcaa
    11641 actatgttta ccgtgaagcg catgatcaaa tcagcaaaag cagtcgtacc gacagcgcga
    11701 ctaaaaccac cattactgaa tataacaacg atggcaaaac caaaacgcat tcgtttaacc
    11761 tcagttttga actggccgaa cccctgcata tccgccaggt agatattaac ccgcaaattg
    11821 tctttagcta tatcaagagc aagggcaact tgtcgttaaa caatggttat gaggagagca
    11881 ataccggtga taaccaggtg gtttataacg gtaatctggt ctcttacgat agcgttccag
    11941 tggcagattt taataaccca ttaaagatct ccttaaacat ggatttcacg catcaaccga
    12001 gcgggttagt gtgggcgaat acgctggcct ggcaagaagc gcgtaaagct cgcattatcc
    12061 ttggtaagac aaatgcgcaa tacatcagcg aatattcaga ttacaagcag tatgttgacg
    12121 aaaaactgga tagcagcctg acctgggaca cccgcttgtc ctggacgcca caatttctga
    12181 aacaacaaaa cctgacgatc agtgccgata ttctcaatgt actggatagc aaaaccgctg
    12241 ttgatacaac gaacaccggt gtggcgacct acgccagtgg ccgtactttc tggcttgatg
    12301 tcagcatgaa attttaatag ttcggatttt acttaaccgg gtaaattcgc ccggttttcg
    12361 catggagata attatgagaa acctctgttt cttactgacg ttagtggcaa ctctgttgct
    12421 ccccgggcgg ctgattgccg ccgccttaca gcaggatgaa aagttaatta ccgggcaact
    12481 ggacaatggc ttgcgatata tgatttatcc gcatgctcat ccaaaggatc aggtaaattt
    12541 atggctgcaa attcataccg gttcattgca ggaagaagac aatgagcgcg gcgtggctca
    12601 ttttgtagaa catatgatgt ttaacggcgc aaaaacatgg ccgggtaata aagtcatcga
    12661 aacatttgag tcaatgggcc tgcgttttgg tctcgatgtt aatgcctata ccagctatga
    12721 cgaaacggtg tatcaggtga gtttgccgac tacgcagaaa caaaatctgc aacaagtgat
    12781 ggcaatcttc agtgaatgga gtaatgccgc aacctttgaa aaactcgaag tagacgctga
    12841 acgtggcgta atttctgagg aatggcgtgc ccatcaggat gcgaaatggc gcacctctca
    12901 ggcgcgccgc cctttcctgc tggctaatac ccgtaattta gaccgtgaac ctatcggcct
    12961 gatggatact gtcgccacgg tcacaccggc acaattgcgc caattttatc aacgctggta
    13021 tcaaccaaat aatatgacct ttatcgtggt cggcgatatc gacagtaaag aagcgctggc
    13081 gctgataaag gataatttaa gtaagtttcc ggctaacaaa gcagctgaaa atcgcgtctg
    13141 gccgacaaaa gccgaaaacc acctgcgctt taatatcatt aatgataaag aaaaccgggt
    13201 gaacggcatc gcactctatt atcgcctgcc aatggtacaa gtgaacgatg agcaaagctt
    13261 tgtcgaacaa gctgaatgga gcatgttagt tcagctgttc aatcaacgtc tgcaggaacg
    13321 catacagtcg ggcgagttga agactatttc tggcggcact gcgcgcagcg ttaaaattgc
    13381 acccgattat cagtcgctgt ttttccgtgt aaatgcacga gacgataata tgcaggatgc
    13441 tgcgaatgca ttaatggcag agttggcaac cattgatcag catggttttt ctgctgaaga
    13501 actcgatgat gtcaaatcta cccgcctcac ctggctgaaa aatgcggttg atcagcaagc
    13561 tgaacgtgat ttacgtatgc tgaccagtcg cctggcatcc agctcattaa ataatacgcc
    13621 gttcttgtcg ccggaagaga catatcaact ttcgaaacgt ctgtggcagc aaattaccgt
    13681 gcaaagtctg gcggaaaaat ggcagcagtt aagaaagaac caggacgcat tttgggagca
    13741 aatggtaaac aatgagcttg ccgccaaaaa agcattgtct cctgcggcta tcctggcgct
    13801 ggaaaaagag tacgccaaca aaaagctggc ggcttacatc ttcccaggca gaaatttatc
    13861 gttaacagta gacgctgacc cacaggcgga aattagcagc aaagaaacgc tggctgagaa
    13921 tctgacatca ttaacacttt ccaatggtgc caaggttatt ctggcaaaat ccgcgggtga
    13981 agagcaaaag ctacaaatta ttgccgtatc gaataaaggc gatttaagtt tccctgcgca
    14041 gcaaaaatca cttatcgcgc tggcaaataa agcagttagc ggaagcggcg ttggcgaact
    14101 ctcctcttcc agcctgaaac gctggagtgc ggaaaactcg gtaaccatga gcagtaaagt
    14161 cagtggcatg aatacgttgc tctccgttag cgcgcggact aataaccctg aacctggttt
    14221 ccagttgatt aaccagcgaa tcacccacag cacgattaac gacaatattt gggcatcgct
    14281 acaaaatgct caaattcagg cgttgaaaac gctcgaccag cgtccagcgg agaaattcgc
    14341 ccagctgatg tatgagacgc gctatgctga tgaccgcacg aaattactgc aagaaaatca
    14401 gattgcacag tttactgccg cagatgcgct ggctgccgat cgccagttgt tttcatctcc
    14461 agcggatatc acgtttgtca ttgtcggtaa tgtcgcagaa gacaaactcg tggcgttaat
    14521 tacgcgttac ttaggctcaa tcaaacactc tgattcgcca ttagccgcag gtaaaccatt
    14581 aactcgcgcg acggacaacg catcggttac tgtaaaagaa caaaatgaac ctgtggcaca
    14641 ggtttcacag tggaagcgtt atgattcccg gacacctgtt aatctggcga cgcgtatggc
    14701 gctcgatgct tttaacgtcg cactggcaaa agatctacgc gttaatattc gtgaacaggc
    14761 atctggggca tacagcgttt cttctcgcct ctcggttgat cctcaggcta aagatatcag
    14821 tcatttgctg gcttttactt gtcaaccaga acgacatgat gaacagttaa cgttagcgaa
    14881 tgaagtgatg gttaagcgtt tgactaaggg gatcagtgag caagaactga acgaatacca
    14941 gcaaaacgtt cagcgcagcc tcgatatcca acagcgtagc gttcaacaat tagcgaacac
    15001 tattataaat agtcttattc aatatgacga tcctgcagca tggactgagc aggagcaatt
    15061 gttgaaacaa atgacgatag agaatgttaa cactgccgtt aaacaatatc tttctcatcc
    15121 ggtaaatact tataccggag tattattgcc aaaataataa cagccccgtc aacacatcgt
    15181 tggcggggat tttagcaata ttcgctattt ttatgtaata attttataaa tgcgttcaaa
    15241 ataataacca agtactaata gtgatatttt aatgtctgat ttttacgtga taattcagga
    15301 ggcacagaaa gcgcataaaa ataacaacat aaaacacctt gccaccaccc aagaattctc
    15361 tattgcattg tttttaatga aaaaaatatc attccaacaa tatctcacga taaataacat
    15421 taggattttg ttatttaaac acgagtcctt tgcacttgct tactttatcg ataaatccta
    15481 cttttttaat gcgatccaat cattttaagg agtttaaaat ggataagaag caagtaacgg
    15541 atttaaggtc ggaactactc gattcacgtt ttggtgcgaa gtctatttcc actatcgcag
    15601 aatcaaaacg ttttccgctg cacgaaatgc gcgacgatgt cgcatttcag attatcaatg
    15661 atgaattata tcttgatggc aacgctcgtc agaacctggc cactttctgc cagacctggg
    15721 acgacgaaaa cgtccacaag ttgatggatt tatccattaa caaaaactgg atcgacaaag
    15781 aagaatatcc gcaatccgca gccatcgacc tgcgttgcgt aaacatggtt gccgatctgt
    15841 ggcatgcgcc tgcgccgaaa aatggtcagg ccgttggcac caacaccatt ggttcttccg
    15901 aggcctgtat gctcggcggg atggcgatga aatggcgttg gcgcaagcgt atggaagctg
    15961 caggcaaacc aacggataaa ccaaacctgg tgtgcggtcc ggtgcaaatc tgctggcata
    16021 aattcgcccg ctactgggat gtggagctgc gtgagatccc tatgcgcccc ggtcagttgt
    16081 ttatggaccc gaaacgcatg attgaagcct gcgacgaaaa taccatcggc gtggtgccga
    16141 ctttcggcgt gacctacacc ggtaactatg agttcccgca accgctgcac gatgcactgg
    16201 ataaattcca ggccgacacc ggtatcgaca tcgacatgca catcgacgcc gccagcggtg
    16261 gctttctggc accgttcgtc gccccggata tcgtctggga cttccgcctg ccgcgtgtga
    16321 aatcgatcag tgcttcaggc cataaattcg gtctggctcc gctgggctgc ggctgggtta
    16381 tctggcgtga cgaagaagcg ctgccgcagg aactggtgtt caacgttgac tacctcggtg
    16441 gtcagattgg tacttttgcc atcaacttct cccgcccggc gggtcaggtg attgcacagt
    16501 actatgaatt cctgcgcctt ggtcgtgaag gctataccaa agtacagaat gcttcctacc
    16561 aggtcgctgc ctatctggcg gatgagatcg ccaaactggg gccgtatgag ttcatctgta
    16621 ccggtcgccc ggacgaaggc atcccggcgg tttgcttcaa actgaaagat ggtgaagatc
    16681 cgggatacac cctgtacgac ctctctgaac gtctgcgtct gcgcggctgg caggttccgg
    16741 ccttcactct cggcggtgaa gccaccgaca tcgtggtgat gcgcattatg tgtcgtcgcg
    16801 gcttcgaaat ggactttgct gaactgttgc tggaagacta caaagcctcc ctgaaatatc
    16861 tcagcgatca cccgaaactg cagggtattg cccagcagaa cagctttaaa catacctgat
    16921 aacgtttaac ggtaacggtg tcccgaaacg aacccgtttc gggataattt ccaaagtctg
    16981 ttcactggca ttagcaacgg aaaatattgt tctgaatacg cttcagaaca aaacaggtgc
    17041 ggttccgaca ggaataccgt tttaggggga taatatggct acatcagtac agacaggtaa
    17101 agctaagcag ctcacattac tcggattctt tgccataacg gcatcgatgg taatggctgt
    17161 ttatgaatac cctaccttcg caacatcggg cttttcatta gtcttcttcc tgctattagg
    17221 cgggatttta tggtttattc ccgtgggact ttgtgctgcg gaaatggcca ccgtcgacgg
    17281 ctgggaagaa ggtggtgtct tcgcctgggt atcaaatact ctgggtccga gatggggatt
    17341 tgcagcgatc tcatttggct atctgcaaat cgccattggt tttattccga tgctctattt
    17401 cgtgttaggg gcactctcct acatcctgaa atggccagcg ctgaatgaag accccattac
    17461 caaaactatt gcagcactca tcattctttg ggcgctggca ttaacgcagt ttggtggcac
    17521 gaaatacacg gcgcgaattg ctaaagttgg cttcttcgcc ggtatcctgt tacctgcatt
    17581 tattttgatc gcattagcgg ctatttacct gcactccggt gcccccgttg ctatcgaaat
    17641 ggattcgaag accttcttcc ctgacttctc taaagtgggc acactggttg tatttgttgc
    17701 cttcattttg agttatatgg gcgtagaagc ttccgcaact cacgtcaatg aaatgagcaa
    17761 ccccgggcgc gactatccac tggctatgtt actgctgatg gtggcggcaa tctgcttaag
    17821 ctctgttggc ggtttgtcta ttgcgatggt cattccgggt aatgaaatca acctctccgc
    17881 aggggtaatg caaaccttta ccgttctgat gtcccatgtg gcaccggaaa ttgagtggac
    17941 ggttcgcgtg atctccgcac tgctgttgct gggtgttctg gcggaaatcg cctcctggat
    18001 tgttggtcct tctcgcggga tgtatgtcac agcgcagaaa aacctgctgc cagcggcatt
    18061 cgctaaaatg aacaaaaatg gcgtaccggt aacgctggtc atttcgcagc tggtgattac
    18121 ttctatcgcg ttgatcatcc tcaccaatac cggtggcggt aacaacatgt ccttcctgat
    18181 cgcactggcg ctgacggtgg tgatttatct gtgtgcttat ttcatgctgt ttattggcta
    18241 cattgtgttg gttcttaaac atcctgactt aaaacgcaca tttaatatcc ctggtggtaa
    18301 aggggtgaaa ctggtcgtgg caattgtcgg tctgctgact tcaattatgg cgtttattgt
    18361 ttccttcctg ccgccggata acatccaggg tgattctacc gatatgtatg ttgaattact
    18421 ggttgttagt ttcctggtgg tacttgccct gccctttatt ctctatgctg ttcatgatcg
    18481 taaaggcaaa gcgaataccg gcgtcactct ggagccaatc aacagtcaga acgcaccaaa
    18541 aggtcacttc ttcctgcacc cgcgtgcacg ttcaccacac tatattgtga tgaatgacaa
    18601 gaaacactaa ccatgtctga atggttataa gacaagggag cgataattca tcgctccctt
    18661 tttcgtgctt gctgtcgtat tgacttaacc gggataagta cgagaatgag cgcacatctg
    18721 tttaccggaa accagcacat atggatatct gctcccgaaa caagaaatta acgattagaa
    18781 gaccagcgat actggttgca ttggcacttt tactgtgtag ttgtaaaagc acgcctccag
    18841 agtccatggt gacaccacca gcaggttcaa agccaccagc cacgacgcaa caatcgtcac
    18901 aaccgatgcg tggcatctgg ctggccacgg tttctcgcct cgactggcca ccggtttcct
    18961 cggttaacat tagtaatccc accagccgag cccgtgtaca acaacaggcg atgatcgaca
    19021 aactggatca tctccaacgt ctcggcataa acacggtctt tttccaggtc aagccggacg
    19081 gtaccgccct gtggccatcg aaaattttgc cgtggtccga tcttatgacc ggtaagattg
    19141 gtgaaaatcc gggttacgat ccgctgcaat tcatgctcga cgaagcccac aagcgtggga
    19201 tgaaagtaca cgcctggttt aacccctatc gcgtatcggt taatacgaag cccggtacta
    19261 tcagggaact gaatagcact ctgtctcaac aaccggcgag cgtctatgtg caacaccgtg
    19321 actggatcag aacgtctggc gatcgctttg tcctcgaccc gggcatccct gaggttcagg
    19381 actggatcac atcaatagtc gctgaagtgg tttcccgcta tccggtagat ggcgtgcagt
    19441 ttgacgacta tttctatact gaatcaccgg gttcacggct aaatgataac gaaacgtacc
    19501 gtaaatacgg aggcgcattt gcgtcaaaag cagactggcg gcgcaacaat actcagcagt
    19561 taattgcaaa ggtatcacac accattaaaa gcattaagcc gggagtcgaa tttggcgtta
    19621 gcccggcagg cgtgtggcgt aaccgatcac acgatccgct cggttccgat acccgaggcg
    19681 cggcagccta tgacgaatcc tacgctgaca cccgtcgatg ggtggaacaa ggattgctgg
    19741 attacattgc tccccaaatt tactggccgt tctcacggag tgccgcgcgt tatgacgtgt
    19801 tggcaaaatg gtgggcggat gtcgttaaac cgaccaggac ccgcctgtat atcggtatcg
    19861 ccttctataa agtgggtgaa ccttcaaaga tagagccaga ctggatgatt aacggcggcg
    19921 taccggaact gaaaaagcag ctcgatctta acgatgcggt accagaaatt agcggcacca
    19981 tcttgttccg tgaggactat ctgaataaac cgcagactca acaagcggtc agctatctgc
    20041 aaagtcgttg gggcagttaa actaccatgc ttactttgta acaagccggt gctttaccca
    20101 ccggcttgtt acaccttgtg aaatatccca ctagtaatca tgcttacata agtcaaatta
    20161 accacactta gttaaacagc tataaatccc taataaaaat gtatgaatat gaaaaaccat
    20221 acgatattcg ctgtcaccga aaaataatta cgatgggcta tactcttctg aagaacagta
    20281 gcgtagattg aaactgtctt aagtcgctta tttcttttca gtatatcttc atatttcagg
    20341 agaatatggt gcagttagtc gatctcgcac gttgtgtttc gttttgattc cgtctgattt
    20401 cccagcctta taagggtgtg aattatggag atgtatttta aaagaatgaa agatgagtgg
    20461 accggacttg tcgaacaggc agatcctctc attcgtgcta aagccgcaga aattgccctt
    20521 gcgcatgctc attatctgag tatcgagttt tatcgaattg tccgcatcga cccgcatgcc
    20581 gaagaattct tgagtaatga acaagttgag cggcagttga aaagtgcgat ggaacgctgg
    20641 attattaacg tgctttctgc ccaggttgac gatgtcgaaa ggctaataca aatccagcat
    20701 accgtcgcgg aagtgcatgc ccgcatagga attccggtag aaattgtcga gatggggttt
    20761 cgggtgctga aaaaaatcct ctatccggta atcttctctt cggattattc cgccgcagaa
    20821 aaacttcagg tctaccattt ctcgattaac agtattgata tcgcgatgga agtgatgacc
    20881 cgcgcgttta cctttagtga cagtagtgcc tcaaaggaag atgaaaacta tcgtatcttc
    20941 tcgttactgg aaaacgccga agaagaaaaa gaacggcaaa tagcctcaat actttcatgg
    21001 gaaatagata ttatctataa agtcctgctg gattctgatt taggcagcag tttgccttta
    21061 agccaggctg attttggcct gtggtttaac cataaaggtc gacattattt tagcggcatt
    21121 gccgaagtgg gccatatctc ccgtctgatt caggatttcg acggtatttt caatcaaacc
    21181 atgcgtaaca ccaggatttt gaataacaga agtttgcggg tgaaatttct attacagata
    21241 agaaataccg tatcgcagat tattaccttg ctgcgtgaat tgtttgaaga agtatcgcgc
    21301 cacgaagtcg gtatggatgt actgacgaaa ttacttaacc gccgtttcct gccgactatc
    21361 ttcaaacgcg aaattgccca tgccaaccgg accggtacac cgctgtcagt gctgattatt
    21421 gacgttgata aattcaaaga gatcaacgat acgtggggcc ataacactgg cgatgaaatt
    21481 ctgcgtaaag tctcattcct ctcgcagaag agattggtga aatcgaaaat attgggcgct
    21541 gggtcatcgc ggaagcttgc cgtcagttag cagaatggcg tagccagaat attcatatcc
    21601 cggcgttatc cgtgaacttg tcggcgctgc actttcgcag taatcagctg cctaatcagg
    21661 tgtctgatgc aatgcaagcc tggggtattg acggccacca gctgacggtg gaaatcacgg
    21721 aaagcatgat gatggaacac gataccgaaa tctttaagcg cattcagatc ctgcgtgata
    21781 tgggcgtggg tttatcggta gatgattttg gcacgggctt ttccggatta tcccgcttag
    21841 tcagtcttcc ggtaacggaa atcaaaattg acaaaagctt tgtcgatcgt tgtctgacgg
    21901 aaaaacgcat cctggcctta cttgaagcca ttaccagcat tgggcaaagc ctcaatttaa
    21961 ccgtcgtggc ggaaggggtc gaaaccaaag agcaatttga gatgctacgt aagatccact
    22021 gtcgcgttat tcagggatat ttcttttccc gcccccttcc cgccgaagaa attccaggct
    22081 ggatgagcag cgtgttaccg ctgaaaatct gacaaattcc tctcgcccgc actcgcgggt
    22141 tttcctttaa ggtgacactg tcacttaaat actgtgattt cagctacgat tccgggagat
    22201 tccttcctta actttccctc ttgcaccaaa actgcactac gctgcacaaa tgttgatcaa
    22261 aagttaaaaa atataacttt aagccagcat tctggcgctt atcccggcat ggcatgagat
    22321 ctgcataagc ggaaagcgca gcaatttttg tcttatacag gaaccgttta tgtcggatac
    22381 caccgaactg gttgatttag ccgtgatctt ccctgatctg gagatcgaat tgaaatatgc
    22441 ctgcgttgat aacatcacag gtaaagctat ttatcagcaa gcgcgttgtc tgctacataa
    22501 agatgcgatt accgcgctgg cgaaaagtat cagtatcgcc cagctgtcag gcttacaact
    22561 ggtgatttac gatgcgtatc gcccacaaca agcacaggcg atgttgtggc aagcctgccc
    22621 agacccgcaa tatgttgttg atgtgacggt cggttctaat cacagccgtg gcacggcgct
    22681 cgacctgacg cttcgtgatg acgtggaggt ggtgaatgat gctaagcgag gaaacgtccg
    22741 ccgtacgccc acaaaaacaa acgcgattta acggtgcaaa actggtatgg atgctgaaag
    22801 gcagtccgct caccgtgacc ggcgcagtca tcattgtatt aatgcgactg atgatgattt
    22861 tttcaccgtg gctggcgacg catgatccca acgccattga tttaaccgcc cgccttttgc
    22921 cgccttctgc ggcgcactgg tttggcaccg atgaagtggg acgcgatctg tttagccgcg
    22981 tactggtcgg cagtcagcaa tcaattctcg ccggattagt ggtggtcgcc attgcgggta
    23041 tgattggttc gctactcgga tgtctatccg gtgtgcttgg cggacgcgca gacgccatca
    23101 tcatgcgcat catggacatt atgctgtcga ttccttcgtt ggtactgaca atggcactgg
    23161 ctgccgctct cgggccgagt ttatttaacg ccatgctggc gattgctatt gtgcgaattc
    23221 ccttttatgt gcgcctggcg cgggggcaag cattagttgt acgccagtat acctatgttc
    23281 aggcggcgaa aacctttggc gcgtctcgtt ggcatctgat caactggcat attttacgta
    23341 actccctacc gccgctgatc gtgcaggcat cgctggatat cggtagcgcg attttaatgg
    23401 ccgccacgtt gggatttatt ggcctgggtg ctcaacaacc gagtgctgaa tggggcgcga
    23461 tggtagcgaa tggtcgcaac tatgtactcg atcagtggtg gtattgcgca tttccggggg
    23521 cagcgatttt gcttaccgcc gtcgggttta atctctttgg tgatggtatt cgcgatctgc
    23581 ttgacccgaa agcaggagga aagcagtcat gacccaaccc gttctggaca ttcaacagct
    23641 gcatttgagt ttccccggtt ttaacggtga tgttcacgcg ctcaacaatg tgtccttgca
    23701 gattaaccgc ggcgaaattg tcggtctggt gggagaatcc ggctcaggta aatcagtcac
    23761 cgcaatgctg attatgcgtc tgctaccgac gggcagttat tgcgtacatc gggggcagat
    23821 ttcactgctg ggagaagatg ttcttaacgc tcgggaaaaa cagcttcgtc agtggcgcgg
    23881 cgcacgagtg gcgatgatct ttcaggaacc gatgaccgcc cttaatccga cacgtcgaat
    23941 aggtcttcag atgatggacg taatccgcca tcatcaacca ataagtcgtc gggaagccag
    24001 agctaaagcg attgccctgc tggaagagat gcaaatcccg gatgccgtgg aagttatgtc
    24061 gcgctatccg tttgagcttt caggtggtat gcgccagcgg gtaatgattg cgctggcatt
    24121 ctcctgcgag ccgcaattga ttattgccga cgaaccgacg acggcgctgg acgtcacagt
    24181 acagttgcag gtactgcgtt tgcttaaaca taaagcacgc gccagtggaa ctgcggtact
    24241 gttcatcagc catgatatgg ccgtggtgtc gcaactgtgc gatagcgttt acgtgatgta
    24301 tgccggaagc gtaattgaaa gcggcgggac ggcagacgtt atccatcatc cccggcatcc
    24361 gtataccatt ggtttgctgc aatgcgcacc agaacatgga gtaccacgcc agccattacc
    24421 cgccattcca gggacggtac caaacctcac ccatttgcct gacggctgcg cttttcgcga
    24481 tcgttgctat gcggcaggtg cacagtgtga aaacgtcccg gcgctgacag cgtgtggtga
    24541 caacaaccag cactgcgcct gttggtatcc gcagcaggag gtcataagtg tctgacacgt
    24601 tattaacgtt acgcgacgtc catatcaatt ttccggcccg taaaaactgg cttggtaaat
    24661 ctacggaaca tgttcatgcc attaatggta ttgatttaca gatccgccgt ggtgaaacct
    24721 tagggatcgt cggcgagtca ggctgcggca aaagcaccct cgcacagctt ttaatgggca
    24781 tgctgcaacc gagccacggg cagtacatcc gttcaggctc acaacgcatt atgcagatgg
    24841 tgtttcagga cccgctctct tcgcttaatc cgcgcttacc ggtgtggcgc atcatcacag
    24901 aatcgctctg gatagctaag cgtagtagtg aacaacagcg gcgagcgttg gcagaggagt
    24961 tggctgtgca ggtgggtatt cgtccggagt atctcgaccg cctgcctcat gcgttctccg
    25021 gcgggcagcg gcaacgcatc gccattgcca gagcactctc ttcgcagcct gacgtgattg
    25081 tgcttgatga gccaacctcg gcgctggata tctccgtgca ggcgcagatc ctcaatttac
    25141 tggtaacgct acaggaaaat cacgggctga cttatgtgct gatttcacac aatgtctcgg
    25201 tgatacgtca tatgagcgat cgggtggcgg tgatgtatct cgggcagatt gtagaactgg
    25261 gcgacgcgca acaggtgttg actgcttcgg cacatccata cacccgatta ttgctggatt
    25321 ccctccccgc cattgataaa ccgctggagg aagaatgagc attacgtaaa acggagctgc
    25381 cgggaaaccg cacgttgccg caaggctgtt ttttctacga acgttgccct ctggcaaccc
    25441 acggatgtga agtccggcaa tcattagcaa taagggagga cggacgtgag ctccggtgct
    25501 ggcgggcgct gtagagatgc taacaacgca tcaggcattc acgcgcctga cgcgtcatcc
    25561 ggcaatgctt tacgatttca actggtaatc cagcgtaatt tccgctttca gtacctgaga
    25621 gaccgggcat cctgcttttg ctttctggat gatgccgtca aaggtagagg catcaatacc
    25681 cggcaccgca acttcactct tcagtgcgat tttcgtaatc gcaaaaccgg catccacttt
    25741 atccagcgac acatcggcgg tggtatcaat tgatgttggc gtgaatcccg cttcccccag
    25801 cattaatgaa agcgccattg agaaacatgc ggcatgcgct gcgccaatca gttcttcagg
    25861 gttggttcct ttttcgcctt caaaacgcgt gttaaatcca tacggctgtt ggttcagcac
    25921 gccgctctcg gtggatactg ttcccttccc gcgtttgata tcgccttccc agtgtgcctg
    25981 acctttctta tggattgtca ttgttgctct cctgtgggct taaaaaggaa attatagact
    26041 acgtgagaaa cagcacgagg ataagcagga tattccgaat aaaatacggc gaaaatatta
    26101 cttctgcact taaattaaat tcccacaata gataaccggg aatatatatc tcaag
//
LOCUS       DAETPZ010000053        25444 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.053, whole genome shotgun sequence.
ACCESSION   DAETPZ010000053 DAETPZ010000000
VERSION     DAETPZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.053"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            273..614
                     /locus_tag="K0W59_002489"
                     /pseudo
     CDS             273..614
                     /locus_tag="K0W59_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(655..2040)
                     /gene="dcuC"
                     /locus_tag="K0W59_002490"
     CDS             complement(655..2040)
                     /gene="dcuC"
                     /locus_tag="K0W59_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBI3023485.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            2626..3186
                     /gene="pagP"
                     /locus_tag="K0W59_002491"
     CDS             2626..3186
                     /gene="pagP"
                     /locus_tag="K0W59_002491"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HBI3023486.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            3361..3570
                     /gene="cspE"
                     /locus_tag="K0W59_002492"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             3361..3570
                     /gene="cspE"
                     /locus_tag="K0W59_002492"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBI3023487.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(3624..4007)
                     /gene="crcB"
                     /locus_tag="K0W59_002493"
     CDS             complement(3624..4007)
                     /gene="crcB"
                     /locus_tag="K0W59_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HBI3023488.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            4100..4888
                     /locus_tag="K0W59_002494"
     CDS             4100..4888
                     /locus_tag="K0W59_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HBI3023489.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTLGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            5017..5220
                     /gene="tatE"
                     /locus_tag="K0W59_002495"
     CDS             5017..5220
                     /gene="tatE"
                     /locus_tag="K0W59_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HBI3023490.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(5321..6286)
                     /gene="lipA"
                     /locus_tag="K0W59_002496"
     CDS             complement(5321..6286)
                     /gene="lipA"
                     /locus_tag="K0W59_002496"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HBI3023491.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(6495..7448)
                     /locus_tag="K0W59_002497"
     CDS             complement(6495..7448)
                     /locus_tag="K0W59_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBI3023492.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQSVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            complement(7707..8348)
                     /gene="lipB"
                     /locus_tag="K0W59_002498"
     CDS             complement(7707..8348)
                     /gene="lipB"
                     /locus_tag="K0W59_002498"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HBI3023493.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            complement(8449..8712)
                     /locus_tag="K0W59_002499"
     CDS             complement(8449..8712)
                     /locus_tag="K0W59_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HBI3023494.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            complement(8823..10034)
                     /gene="dacA"
                     /locus_tag="K0W59_002500"
     CDS             complement(8823..10034)
                     /gene="dacA"
                     /locus_tag="K0W59_002500"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HBI3023495.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            complement(10173..11261)
                     /gene="rlpA"
                     /locus_tag="K0W59_002501"
     CDS             complement(10173..11261)
                     /gene="rlpA"
                     /locus_tag="K0W59_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HBI3023496.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAQTTLAPGVLEGSEPTPAPQPVVTASSTTPVTSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            complement(11272..12384)
                     /gene="mrdB"
                     /locus_tag="K0W59_002502"
                     /gene_synonym="rodA"
     CDS             complement(11272..12384)
                     /gene="mrdB"
                     /locus_tag="K0W59_002502"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HBI3023497.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSVLVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            complement(12387..14288)
                     /gene="mrdA"
                     /locus_tag="K0W59_002503"
     CDS             complement(12387..14288)
                     /gene="mrdA"
                     /locus_tag="K0W59_002503"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HBI3023498.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            complement(14319..14786)
                     /gene="rlmH"
                     /locus_tag="K0W59_002504"
     CDS             complement(14319..14786)
                     /gene="rlmH"
                     /locus_tag="K0W59_002504"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBI3023499.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVILDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            complement(14790..15107)
                     /gene="rsfS"
                     /locus_tag="K0W59_002505"
                     /gene_synonym="rsfA"
     CDS             complement(14790..15107)
                     /gene="rsfS"
                     /locus_tag="K0W59_002505"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBI3023500.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            complement(15367..15978)
                     /locus_tag="K0W59_002506"
     CDS             complement(15367..15978)
                     /locus_tag="K0W59_002506"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HBI3023501.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            complement(16002..16643)
                     /gene="nadD"
                     /locus_tag="K0W59_002507"
     CDS             complement(16002..16643)
                     /gene="nadD"
                     /locus_tag="K0W59_002507"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBI3023502.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            complement(16645..17676)
                     /gene="holA"
                     /locus_tag="K0W59_002508"
     CDS             complement(16645..17676)
                     /gene="holA"
                     /locus_tag="K0W59_002508"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBI3023503.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            complement(17676..18257)
                     /gene="lptE"
                     /locus_tag="K0W59_002509"
     CDS             complement(17676..18257)
                     /gene="lptE"
                     /locus_tag="K0W59_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HBI3023504.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            complement(18272..20854)
                     /gene="leuS"
                     /locus_tag="K0W59_002510"
     CDS             complement(18272..20854)
                     /gene="leuS"
                     /locus_tag="K0W59_002510"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBI3023505.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATLEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            21089..21571
                     /locus_tag="K0W59_002511"
     CDS             21089..21571
                     /locus_tag="K0W59_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HBI3023506.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTELDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            complement(21641..22624)
                     /locus_tag="K0W59_002512"
     CDS             complement(21641..22624)
                     /locus_tag="K0W59_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBI3023507.1"
                     /translation="MIIIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAID
                     SPDDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFA
                     SNALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFW
                     YKQAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNA
                     GEGVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECN
                     DATAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            22782..23489
                     /locus_tag="K0W59_002513"
     CDS             22782..23489
                     /locus_tag="K0W59_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBI3023508.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWILSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            23486..24913
                     /locus_tag="K0W59_002514"
     CDS             23486..24913
                     /locus_tag="K0W59_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HBI3023509.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(24923..25441)
                     /locus_tag="K0W59_002515"
     CDS             complement(24923..25441)
                     /locus_tag="K0W59_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBI3023510.1"
                     /translation="MVAAITCISQPKKTTLRDKAMVNYAFDYLRSPGSLPLTTAATEL
                     SAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGL
                     GVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQKWLKLAAKQHYK
                     NAERLLAGLPAH"
BASE COUNT         6359 a         6253 c         6320 g         6512 t
ORIGIN      
        1 atgagctgat ttgtgatatg agtcatttgg tgacagcctg gttgttatcg gttttgaacg
       61 ttttttgaag ctatgattga aatgatttat agtgtttttg tttttcgttt tttcaatgaa
      121 acaaaagcaa ttttttacta tctttcgcgt ttcattggtt aacgcctaaa atttgtacta
      181 caggcttgca agccccacca aatctaatcc catcagatcc cgaataatcc aatgttctcc
      241 cggttttttc gtacttcaag tgaatcaata cactggggca aacgctaacc cgtttccgcc
      301 agcgtaaaca tatgctggaa agtattgata gcgccagcca gaagcaaatt gatgagatgt
      361 ttaatgccta tgctcgcggt gaacccaaag acgaactgcc gaccggcatt gaccccttga
      421 cgctaaacgc ggtgcgaaaa ctgtttaaag agcctggtgt gcaacatacg gcagaaacgg
      481 tggcgcaggc actgaccatc agccgcacca ctgccaggcg ttatcttgaa tattgcgcca
      541 gccgccatct gattattgct gaaattgttc acggcaaagt tggcagacca caacgcatat
      601 accacagtgg gtgacatgat aaagccggag gaaacttgcc tccggcatta ctatttactt
      661 gcctgtgacc gctgctgccg ttcctggcac catcagctct gtagcaacga taacaatcac
      721 tagcccaaca agcaccggca ccgaggtgcg ttttacgact tcaaatggtg agattttcgc
      781 catcccggca accgcaacga ctacgccaga aacgggtgat agggtacgac ccaggtttga
      841 cgcctgcagc atcgggatag tcaaatacgc cgggttaatg ccggaagagt gcgccagttt
      901 cgggatcatc tcaacaaacg cataaaacgg cgcattgcct gaaccggtcg tgactgccgc
      961 cagcatggtc agaatcacca ataccagcat caggatgata ctcgccgaac caaacgaggt
     1021 agcgatagaa atcagacttt gaataaagcc gatggtgcta agcccctgag cgaatacccc
     1081 agcggcaacc agcagcatca ccacgttagc aaacgcatct gccatcccgc gataagccac
     1141 ttccagacca gagaaaactt tctgggtatt aaagctgcgg aggaactcca gaatggaggc
     1201 aatcagcata caaatcacca gaatagtgat gatgtgtaat tgcggacccc atttaccgtc
     1261 aaaaatcagt acaccgatga tcggcgtgaa cggcaaaatg gcataaaacg cgggggcagt
     1321 ggtggtgatt tcactgacat ctaacatttc atgagagatg tgctcttttt tatccagata
     1381 acgttgccag aagaagtggg cgatcgccat gccgataatt gcagcaattg agataggcag
     1441 cgtcgttttg aaggcgaagt caatcagcga catttcggaa gcctgcgccg ccagcaccac
     1501 atcccctgaa gtcggtgcga gaataatcgc cgccggggag gcacaaatgg cagctgctgc
     1561 gccacgactg ataccaacgt ttaccatcac cggaaacagg gttgccatca gcaaaacacc
     1621 cagaccggtt gcggaagaga cggccagtga catcagacaa gcaacaaaat aggcggcaat
     1681 catcagcagg taaggggagt taatatactg caatggtttt gacgccagct tgaccaccat
     1741 atcattcgcg ccgatatggg tcatgtaagc ggcaaagcca cacagcatca taatcatcat
     1801 gccgaggtcg ccgccgcggc tcattagtaa tattttaacg tattcaacga tatccgtggc
     1861 gctgtagcct gttgaagcct ggctggacgg taacactttg tgccccataa tggcactgat
     1921 aatcagcaat aacaggccac cgacaaataa cacaccagtg gcggaatacc ctttaatgat
     1981 gtagcgagct acacccacaa taaccacaac cccaataagg agttcaatga atgtcagcat
     2041 aatttttcct gtctccaggc ccaaagtaaa taataaaaac ttcctaaagc ttaaggaaaa
     2101 aatatgccca ataaattggc gatgaatgct gattaaaatc aagaaaaact gccattaaga
     2161 cattgaagtt gctgttttta tacatagata acaaaacgca tatttagcgg ttggcaatta
     2221 tcacataata atgtgtgtaa attgatggtg gattgatgca aatttgttaa ttgcaatggt
     2281 gttagctgcg gctgtgcgct caaaaaataa tctaatatga gcatgggttg acgatatata
     2341 ttatatactt cgttaagatg attgttgtat ctcgttaaaa aaaaataatt tttccttgat
     2401 tgcattttgt catcaaaaaa gacttggttt ttcttttttg actattccca tcgcagaaaa
     2461 cgacgcatca tctttaatcg atgcgcggaa atatttaact tgaacaagcg gaaataaata
     2521 gagcagctat tcagattatt ctttatgttg ggtctattaa ggttatgtta attgtagctt
     2581 tgctatgcta gtagtagatt tttgataaat gttttatggt cacaaatgaa cgtgagtaaa
     2641 tatgtcgcta tcttttcctt tgtttttatt cagttaatca gcgttggtaa agtttttgct
     2701 aacgcagatg agaggatgac aacgtttaga gaaaatattg cacaaacctg gcaacagcct
     2761 gaacattatg atttatatat tcctgccatc acctggcatg cacgtttcgc ttacgacaaa
     2821 gaaaaaaccg atcgctataa cgagcgaccg tggggtggcg gttttggcct gtcgcgttgg
     2881 gatgaaaaag gaaactggca tggcctgtat gccatggcat ttaaggactc gtggaacaaa
     2941 tgggaaccga ttgccggata cggatgggaa agtacctggc gaccgctggc ggatgaaaat
     3001 tttcatttag gtctgggatt caccgctggc gtaacggcac gcgataactg gaattacatc
     3061 cctctcccgg ttctactgcc attggcctcc gtgggttatg gcccagtgac ttttcagatg
     3121 acctacattc cgggtaccta caacaatggc aatgtgtact ttgcctggat gcgctttcag
     3181 ttttgaggca aatgaagttt tagtaacttc tttaaaatca ataactaaaa taagcaacat
     3241 caaaaataac gcgactttta tcactttttg gtaaagttgc actggacaaa gcgtaccaca
     3301 attggtgtac tgataaccga cacagcattt gtgtctattt ttcatgtaaa ggtaattttg
     3361 atgtctaaga ttaaaggtaa cgttaagtgg tttaatgagt ccaaaggatt cggtttcatt
     3421 actccggaag acggcagcaa agacgtgttc gtacacttct ctgcaatcca gactaatggt
     3481 tttaaaactc ttgctgaagg tcagcgcgta gagttcgaaa tcactaacgg tgccaaaggc
     3541 ccttctgctg caaacgtaat cgctctgtaa gatacgtcag caagaattca aaacccgctt
     3601 aatcagcggg ttttttttgg tctttagtgt gcggttgagg ccgaaaacag ccagaatgcc
     3661 agtgcggtca tggcaaaaga ccccagaagg ttgacgaaaa cgttcagtaa tgcccagcca
     3721 aagcggccct cttgtaacaa aaacaccact tctgccgaaa atgttgagaa ggttgttaga
     3781 ccgccacaaa atccggtggt gattaatact ttccacactg gatcaatgtt cgtcatcctg
     3841 ctgaaccagg cgaatcccat tcctatgatg aatgccccaa tcaggtttgc tgccagcgtc
     3901 cccaacggaa tcgcctgatg cagcgggtta aatcgcatac ttaacagcca tctcgctacg
     3961 cttcccgtac caccgccaat aaaaactgct aaaagaagtt gtaacactgc aaaatcctgc
     4021 tatttgattt gtatgagtga taagtgtaac gccgaataat cgtcgttggc gaattttacg
     4081 actctgacag gaggtggcaa tgctggttgc tgcaggacag tttgctgtta catctgtgtg
     4141 ggaaaagaac gctgagattt gtgcctcgtt gatggcgcag gcggcggaaa acgacgtatc
     4201 gctgtttgtc ctgccggaag cattgctggc gcgcgatgat catgatgcag atctatcggt
     4261 taaatcagca cagctgctgg aaggcgaatt cctcggacgt ttacggcgag aaagtaaacg
     4321 taacatgatg acgacaattc tgacgattca tcttccttca acgctggggc gcgcatggaa
     4381 tatgctggtg gcacttcagg caggaaacat cgtcgcccgt tatgccaaac tgcatctcta
     4441 tgatgcattt gccattcagg aatcacgcca tgttgatgct ggtaatgaaa tagctccgtt
     4501 actggaggtg gaagggatga aggtcggtct gatgacctgt tatgacttac gctttccaga
     4561 gctggcgctg gcacaggctt tacagggagc tgaaatcctg gtacttcctg ccgcctgggt
     4621 tcgtgggccg ctcaaagagc atcactggtc aacgttgctt gccgctcgtg cgctggatac
     4681 cacctgttat atggtggcgg cgggggagtg cgggaacaaa aatatcgggc aaagccggat
     4741 tatagatccc tttggcgtca ccattgcggc agcgtcagaa atgcctgcac tcattatggc
     4801 ggaagtgacg cccgaacgtg tgcgtcaggt gcgcgcgcaa ctgcccatct taaacaaccg
     4861 tcgctttgcg ccgccgcaat tattatgatg tttttttact cggcgcttga ttcaccttgt
     4921 tacagattgc tattgtgtgc gcgcgtcgaa tgaccgttaa tattctctgg tttttaaggc
     4981 gcgttctgtt gccggttata tgtcaagaag gtatctatgg gtgagattag tattaccaaa
     5041 ctgctggtag ttgcggcgct ggtcgttctg ctgtttggga ctaagaagtt acgtacgctg
     5101 ggcggagacc ttggagcggc cattaaaggg ttcaagaagg cgatgaatga tgacgatgct
     5161 gcggcgaaaa aaggcgcaga cgttgatctt caggctgaaa agctctctca taaagagtga
     5221 cgtggcgagc aggacgctcc ctcaatatct tgttcgatac aaaaaacccg cttcaaaaag
     5281 cgggtttttt atcagacaga tgtaagtaat tattacagga ttacttaact tccatccctt
     5341 tcgcctgcaa atcggcgtgg taagaagagc ggacaaacgg accgcatgca gcatgggtaa
     5401 agcccatcgc cagcgcttcg gctttcattt cgtcgaactc atccgggcta acgtaacgtt
     5461 gaaccggcag gtgatggcgg cttggctgca aatattgccc cagcgttaac atcgtcacac
     5521 catgacggcg caggtcgcgc attacctcaa taatttcttc attggtttca cccagaccca
     5581 ccatcagacc agacttggtc gggatttccg gatgcgcttc tttaaagcgt tccagcagct
     5641 tcagcgacca gttgtaatct gcgccaggcc gtacctgccg gtaaatacgc ggtacgtttt
     5701 ccaggttatg gttgaacaca tctgggggcg ttgcagtcag aatatccaga gcacgatcca
     5761 ttcgaccgcg gaaatccggc accagagttt caattttgat ttgcgggctt ttttcccgaa
     5821 tggcagtaat acaatccgca aagtgttggg caccgccatc gcgcaggtca tcacggtcaa
     5881 cggaggtgat aaccacataa cgcagcgcca tatcggcaat ggtctgcgcc agtttcactg
     5941 gttcattggc atcaggagct accgggcgac cgtgggcaac atcacagaac ggacaacggc
     6001 gggtacaaat agcgccgagg atcataaacg ttgctgtgcc gtggttgaag cattccgcca
     6061 ggttagggca ggaggcttcc tcgcagacag aatgcaggcc atttttgcgc attgcagctt
     6121 tgatgccctg gatacgtgta gagtccgctg gaagcttgat tttcatccat tccggcttgc
     6181 gcagcagggc ttcgcgctct gttgccacgt ttttaaccgg gataagggcc atcttatcgg
     6241 catcgcggta tttaacaccg cgttccatca caatgggttt actcatagcg tgcgtgttcc
     6301 agttgcgaat tacgaaggaa agcgtttcaa ttcaatagat tgttgtaatt atcaactatt
     6361 tttgaattaa cgactggcag tatatcattg aaacggacct gaaagcagcc aaagcggtcg
     6421 cgaaaatgta aaattgttgt tggattgtgc cattttatcg ttctgcactg tgatctggct
     6481 gtaacaatac tttttcaaaa tgcattacgg ataacatcta ttactccgtg caggatggga
     6541 tcacgcaggc tgaatttgtt gtagtggata gagaaatcta tttgctcctc atttaaggac
     6601 ggaaaaggca gtttttccag cggccagcaa cgactaaaca ggttataaaa acggctggga
     6661 ataatcgcta acatgtcact gttggcaacc agtgcagcga ttgtcaaaat gttgtagctg
     6721 gtgaaattga tttgccggtc cggaaacatc tcttgaactc tctgccgcag accgctgaaa
     6781 ttttgccctt ccggtaacag aagtacatgc gcagcgttgt cgatagtctc tctgtcatct
     6841 tctaaagaga gtagtggatt tcccttgcgg caaattaaca ccatattgtc ggtgaacaga
     6901 acatgatgtt gcaccgtacg attggtgcaa aacatgttat cgatgatgag atcggtttga
     6961 aactgactga gttggttttc cgcgtcgctg atgggcgggt tgcgcagtaa aagctgcgga
     7021 tagtgagttt taatcgcccg atagatgaca ggaaggacca gggctccgac cgagggagtg
     7081 gtcgcaatag ttatcgttcg ttgcttatca tagcttcctt cgatatccag cgcgccaaga
     7141 atggactcaa ggccctgact gatatactca tgtagatgca tcgcaaatgc ggtaggagtg
     7201 acactctggc ctttgcgaat aaacaatggg tcagggaata taacgcgcag tttctgaata
     7261 gactgactga ttgccgaggg ggtcagatta agcactttcg ctgcattaac gatcccttta
     7321 tgtacatata cagcttcaaa aatagtcaga aggttaagat caatatttcg taaggttgta
     7381 aatatttgtg gtttaccttc tgacgacttt ctgcttaaac agggttcaat ttgattatta
     7441 ctatccacgc acttactcca attttattca tggaaaaata atatttaaaa aattacaata
     7501 atcttatgtc taattggaac ggaacgcttt tgctcaccat aatcaactat ttcaataggt
     7561 taatcaatgg ggtgagttac ggagcaaggc gtggcaccgc atttctgcaa gtgataaaga
     7621 aaatatacaa acgcgaagga gatgtaaagc attagcaggt attatgagta atgacccagt
     7681 gtaaattggg ccattgatgt atggaattaa gcggtaatat attcgaagtc cggattgttt
     7741 agtagcgcta aaatattttc cagtaaacgt ggagcaatat tattagtcgt cgcttcgggt
     7801 ttccattgtg atattttagc catttccatt ccggcatacc cacaaggatt aatacgtaaa
     7861 aatggtgaaa gatccatatt gacgtttaat gccagaccgt ggaatgaaca accgcgtcga
     7921 attcgtaaac ccagtgagca aattttcttt tccccgacat agacaccagg cgcgtcagcc
     7981 cgaggatgcg cttctatacc cagttcagcc agggtattca ccactgtttg ctcaagcaag
     8041 gtcaccagtt cacgcacacc gagtttacgg cgtttcaggt taagcaacac atacatcacc
     8101 tgttgtcccg gcccgtgata agtcacctgc ccaccgcgat cgctctggat caccggaata
     8161 tcacccggca ttaaaatgtg ctccgctttt cctgcctgac cttgggtgaa taccggatag
     8221 tgctcgacca gccagatttc atcaagggta ctattatcgc gggtatcggt gaattcatgc
     8281 atagcccggg agattggctc gtaaggctga agaccgagct ggcggacaag aattttatcc
     8341 tgatacaaaa cggcatctcc gtggagaatg agtaaaagtg ggggaaaagt atatcacagc
     8401 gaggagagga gagttacccg accaggagcc gggtaacgga gaagcgagtt acagaaccat
     8461 gcggacaata tcgattttgc ccagttcttc atacagtgtt tcaacctgct cgatatgagt
     8521 ggcgttgata gtgatagata ccgagtggta gttgcctttg ctgcttggtt ttaccgttgg
     8581 ggtgtagtca cctggcgcat ggcgctgtac cacttcaacc acctgatcaa ccagctcagg
     8641 taacgcctgc cccataactt tgtaagtaaa aggagtaggg aattcaagca gttcgttaag
     8701 tttggttttc atgtcagctc cggcgtaacg taattaaata gcaactcccg ccagaaggtg
     8761 ggagtttttt tactgatgct tagtatatgg ggacggaaat tacactttca agtgttcaat
     8821 ttttaaccaa accagtgatg gaacattaat ttaatgtaat caatgatttt gccgaagaag
     8881 ttaccttccg ggatttcttg caacacaacc agcgggcgtt gctcgatcgt tttgccatca
     8941 agctggaagt tgatagttcc gacgacctga ttcttttgca gcggcgcatg caattcactg
     9001 ctgttcagca catagctggc tttcagatct ttcatgcgac cacgcggaat ggtcaggtac
     9061 acgtctttat caacccctaa cgaagcgcga tcagaatcac taaaccaaac cggttctgag
     9121 gcgaactctt tacctacttt cagtgggtta acggtttcga agaaacggaa gccccaggtt
     9181 agcagttttt tactttcggc ttcacggcct ttaaaagtac gtccacccat caccgcagaa
     9241 atcaagcgca tctggccttc agtcgcagaa gcaacaaggt tgtaacctgc tttgtcagtg
     9301 tgtccggttt tgatgccgtc gacattcagg ctgttatccc ataacaggcc gttacggttc
     9361 agctggcgaa taccgttaaa cgtaaattct ttttctttat agatcgagta ttcattcggt
     9421 acgtcacgga tcaatgcctg gccgatcagc gccatatctc gcgcggagct gtactgacca
     9481 tcagcatcca gaccatgtac cgtctggaag tgggtatttt tcaggcccag tgcgttaacg
     9541 tagctgttca tcaagccaac aaaagcgtcc tggctaccag cggcaaaatc ggccatggcg
     9601 acacaagcat cgttacccga ttgcaggtta ataccgcgga tcagctgaga aaccggaacc
     9661 tgcatgcccg gtttgaggaa catcagcgaa gaacctttaa acaccgggtt accggtggcc
     9721 catgcgtcgt tgccgatagt gactaaatca gtttctttaa atttaccggc tttcattgcc
     9781 tggccgataa cgtaactggt catcattttg gtcaggctgg caggatcgcg gcggacatct
     9841 gcgttctgtt cggcgagcac tttgccggag ttatagtcaa tcaggatgta ggactccgca
     9901 tcgatctgcg gtacacccgg gatcatagtt ttgatattca ggtcatcggc atgtgcagca
     9961 gagataaagg ctgtgcaaag agccgtggtg agcgccaggc gcttcatgat acgagcggaa
    10021 aaaatggtat tcatggtctg aactacgaca tccgtgatgg aattaaaaaa gtgccctact
    10081 atagcaaatg cactaccggc aggcatctga ctttccgcgt gactttgtta acgtcattta
    10141 cagaaattga cacatcagat gcctgcttta cgctactgcg cggtagtaat aaatgactgt
    10201 aattgggctt cggtttgcaa acgttgctgc aaggtactgg cttcggcttt gctggcgaat
    10261 gggccaagct ggatccgcca gaccgcgcca ttttgagtta cgcgaccggg gacgccgaac
    10321 ttctgtccca gttgctgttg gtactgttgc gcacgagcct gatcgcttac ggccccgact
    10381 tgcaccataa agttgccgct ggcgctttgc gaggcggctt gcggtgtcac cattgcaggc
    10441 gaggttacag gcgttgtcga cgaagctgta acaacgggct gtggcgcagg cgtcggttcg
    10501 ctgccttcca gtacaccagg cgctaaggtc gtttgagcgc cgaggaaacc gctactggtt
    10561 accggcgcgc cggtcggatc ttcgcttttt agcgtcgaat tactgaccgg aagaatgtca
    10621 ccctgcgggc cagacactga acttgttccc gcgccaccgc ttaaatcggg aggtgcaggc
    10681 agggcataag tctgtttggc gactgtggta caagccatac caggaccaga aagcgaacca
    10741 tcctgggcga caataatcgg atcgatacga actttggtgt tgtttgacgt gttaagacgg
    10801 tcagctgccg cgcgagaaag cgaaataacg cggtcgttgc cgtaagggcc gcgatcatta
    10861 atgcgcacca cgatcattcg cccgttagcc aggttagtga ttctggcgta gctggggatc
    10921 ggaagcgttg gatgggccgc cgtcagcttt gtcggatcga aagcttcgcc agaggccgtc
    10981 aggttactgc ctggttcggc atcatagatt gccgccagtc ccgcctggct aaatcgagac
    11041 ggatcctgca cgattttgta gcttttaccg tcgcgctggt aatcctgatt tgccgtcgcg
    11101 ttcagtggtt cgaaacgcgg gtccgccccg ctaatttcaa ctatggggcc gttacatacc
    11161 gcaggctgcg gtacacttac cgtctgttgc tgaccatcat cgcttgtaca tgccgcgagc
    11221 attcctgccg cgatgcagat cccgagccac tgcttacgca ttgcgcacct cttacacgct
    11281 tttcgacaac attttcctgt gggtgtggat tgacattaca atcccgaacc cagccatcag
    11341 cacaattagc gccgatcctc cataactgac cagtgggagc ggaaccccta caaccggcag
    11401 aataccgctt accataccaa tatttacgaa gacataaacg aataatatca gcattaagcc
    11461 accagccatg acgcgaccaa aggtggtttg cgctctggcg gctatccaca gcccgcgcat
    11521 gatcagcaga atgtagagag cgagcagaat cagaatgccc actaatccca gctcttccgc
    11581 cagtaccgcg aagataaagt cagtatggcg ttcggggaga aattcaagct gtgactgagt
    11641 gccgtgcagc cagcctttgc cgcgtaatcc gccggagcca atagcaattt tagactgaat
    11701 aatgtgatag cccgcgccga gtgggtctga ttccgggtcc aggagcatca ttacgcgctg
    11761 gcgctggtaa tcatgcatca ggaagaacca cagaatcgga atgaacgccg ctaccagcac
    11821 tactgcgacg ccaatcagac gccagctaag gccagagagg aacagtacaa acagaccgga
    11881 aagcgcaacg aggattgatg ttcccaggtc aggctgtgca gccaccagca gcgtgggcat
    11941 aaatatcagc accagcgcga tgccagtgtt cttcaacgat ggcgggcaaa cgtcgcggtt
    12001 gataaagcgc gcaaccatca gtggtacggc tattttggca atttccgacg gctgaaaacg
    12061 aacaataccg aggtccagcc agcgttgagc gcctttagag atggctccga aagcatctac
    12121 cgccaccagc aaaataatac agatgatata gagatagggg gcccagcctt cataaacgcg
    12181 tggaggaatt tgcgccatca ccaccatgat gaccagaccc atcgcgattt ggccgatttt
    12241 acgctccatc atgccaatat cctgaccgct ggcgctccag ataaccagga cgctgtaaac
    12301 cagcaatgcc agtaaaatca gcagcattgt gggatcgaga tggactttat cccagaatgt
    12361 ttttttattc ggattatccg tcatgattaa tggtcctccg ctgcggcaac cgctggattt
    12421 tccgcaggca gatcggtgtt gttatcaccc agcataatgt ggtcgaggat ctggcgcatc
    12481 agcgtaccaa ccgccggacc cgcgccgccg ttctccagaa tcatggcgac agccacttgc
    12541 ggattgttgt atggcgcaaa ggcggtcatc agtttgtggt cacgtaaacg ctcggcaatt
    12601 ttgtgcgcat tataggtttc gttcgctttc agaccgaaga cctgagcggt accagatttc
    12661 gccgcaattt tgtacggtgc gctggcaaag tatttatgcg ccgtaccatt agggcggtta
    12721 gcaacaccgt acataccgtc tttcgccagc tcccagtaac cggaatgaat atcgccgacg
    12781 ggcggttcat gcggttgtac ccatggcacc tgtttgccgt cttcggcggt gctcatcagc
    12841 aaatgcggaa ccttcacgat accgtcatta atcaggatca tcagtgcctt actcatctgg
    12901 attggggtcg ctgtccagta accctggccg ataccaaccg gaatggtgtc accctgatac
    12961 cacggttttt taaagcgttt ctgtttccat tcgcgggtag gcatgttgcc ggaacgttct
    13021 tccgccaggt cgataccggt gtaatgacca taaccgaatt tacccatcca ttcggagagg
    13081 cgatcgatcc ccatatcgta ggccacctga tagaagaagg tatccgcaga ttcttccagc
    13141 gatcttgtga cattcagacg cccgtggccc cattttttcc agtcacgata acgtttttcc
    13201 gaccctggta gttgccacca gcctgggtca aacagcgtcg tattgcgcgt gatcaccccg
    13261 gcgcttaatg ccgaaaccgc cacatagggt ttaactgtag acgcgggagg ataaaccccc
    13321 tgtgtggcgc ggttcaccag cggtgtattc gggtcgttca gcaaggcgga ataatctttg
    13381 ctggagatac cgtcaacaaa caggtttggg tcataactag gcgtggaaac cagcgccagc
    13441 accccacctg tacgcggatc ggtaacaacc acagctgcgc ggctacccgc cagcagcgtt
    13501 tcaatatatt gctggagttt gagatccagc gtcaggtaaa tatcgtgtcc ggcttgcggt
    13561 ggtacttctt ttaactggcg gataacacgc ccacggttgt taacttcaac ctcttcataa
    13621 ccggtctgac cgtgcagcac gtcttcatag taacgctcaa tgcccagctt accaatatca
    13681 tgcgttgccg catagttggc cagtttgccg tcattattca ggcgttcgac gtctttatcg
    13741 ttgattttcg acacatatcc gatgacgtgg gtcaacgccg aaccgtaagg atagtaacga
    13801 cgtttatagc ctttaacttc gacacccgga aaacggtact gattgacggc aaagcgagct
    13861 acttgtactt cggtcaggtt agttttcacc ggaatagagg tgaaacggtg tgaacgtgcg
    13921 cgctcttttc ggaatgcagc aatatcgtca tcggtcagat ctaccacgct gcgcaaagcg
    13981 tccagcgttt gctgcacgtt atcgactttc tccggcatca tttctatctg gtagatagtg
    14041 cggttgaggg ccagagggat accgttacgg tcgtagataa tgccgcggct gggcgcgata
    14101 ggcaccagct taatgcggtt ttcattagag cgggtctggt agtcggtaaa gcgaacaatt
    14161 tgcagattat acaggttggc gataagcacg ccggtcagca gcaaaatccc caaaaaggcg
    14221 accagcgccc ggcgcacaaa cagcgcggac tctgccgtat agtcgcgaaa agagttctgt
    14281 agtttcatcc gctgcgtttt ctactcaaag ctcccttatc actcacggtg ataaggatgg
    14341 ttggtggtga tgctccacgc ccggtacaga ctctctgcga ccagcacgcg aaccagcgga
    14401 tgggggaggg taagcgccga cagcgaccag ctctgctcag ccgccgcttt acaggcaggc
    14461 gacaaccctt caggcccgcc aatcagtaga cttacgtcgc gaccatccag cttccagcgt
    14521 tccagctcag cggctaactg cggcgtatcc cagggcttgc ctggaatatc gaggatgaca
    14581 atgcggtttt tgcctgcggc cgccaacatc tgctcaccct ctttgtcgag tatgcgcttg
    14641 atgtccgcat tcttgccgcg tttgccggcc ggaatttcaa tcagctcgaa gggcatatct
    14701 ttcggaaaac gacgcaggta ctcggtaaaa ccggtttgta cccagtccgg cattttcgtt
    14761 cccacggcga caagttgcag cttcacgcat taactccaga gtttttccag ttcatacagg
    14821 cgacggctct cttcctgcat gacatggaca atcacatcgc ccaaatccac gacaatccag
    14881 tcggcgctgt tttcaccttc tacgccgagc ggtaacaggc ccgctgcgcg agactcctgc
    14941 acaacgtggt cagcaatgga cataacatga cggctggacg tacccgtaca gatgatcatg
    15001 cagtcggtga tgctggattt gccttgaacg tctaaggcga tgatgtcctg acctttgagg
    15061 tcatcaattt tgtcgataac aaaatcctgg agtgctttac cctgcaagtt ttccccctgg
    15121 gtgaatcaaa tagataaaaa tggtctgtca gtatacctga accagaggcg atttcgggac
    15181 aattgtcgcc gaatcggctt tcgaaagtgg gctatcatcc caccccgcgc cgcagattgc
    15241 atcgccattt ttgtaaaaca atttctacaa agtcgtgtct ggcggaaaaa gtctggctgc
    15301 ggagaatatc agcctgcccg gggctgtcaa tggtcgttgc ggcttacccg taaaaaaaca
    15361 gaaaagtcat gcattctcga ccccgatggc acggctattg aggacgcgta gcgtcgcgaa
    15421 tttttggttg atatcaatgg cgctccaaca cccctggtca acgcgaaaat gccacataga
    15481 ctcggcaggc atgccaatta aacgagcaat taacagactc agtacgccct gatggctgac
    15541 gattaaaata ttctgatagt gctgatattc actaagcctt gcgataaagc gttccacacg
    15601 ttgcgaaaat gcctgaaatc cttcaccgtt cgtggggata gcatgctgcc agtcattgca
    15661 ccacgcgcta tagttttcgg catcttcttg catgaggtcg cgatgatgtc gcatctccca
    15721 gtcgccaaaa aacatttcgt tgagttcagg gatgatatga acggggagct ggcggtcact
    15781 gagaaccagt cgcgcggtat gctgtgcccg ttccagttca ctgcataaaa ccaggtcaaa
    15841 ggaaacatca tctagcagtg tatgcagatt ttgcgcttgc tcaataccgc gtgcggtcag
    15901 gggtgtgggc gcatgaccgc tgtacagacc atcgacattc gcttgtgttt cgccatgacg
    15961 aattaaccac agtcgcatca tgccctccgt aacgacaggt atcagcgata caagccttgt
    16021 tggttaatgt aagtcaatac cggttccggc aataaatcct cacacgattc accgttttgc
    16081 aaacgttcgc ggatgatggt cgccgagatg ttaaaccacg gcgtttcagc cagataaatt
    16141 ttaccggcag gctgaaggtg aagatcttcc gggttatgtg tcaaatgatc ttccagccat
    16201 tgctggtatt gcggttgcgc catttcaagt gggtaacctg gacgccgaca gacgatcaaa
    16261 tgtgcattgt cgagtatcgt ttcgtattcg taccaggtcg gaaaggtcag caatgaatcc
    16321 tgaccaataa taaacgccag cggcacgtcc ggtccttgtt cctgccgcca ctctttcagt
    16381 gtttgtgcag tgtaagaggg ggcattgcgc tttagctcgc gttcatcaag agtaaataat
    16441 ggcttgtcgg caatcgccag ttcaagcatg tgtttacgct gcacgctgtt cgcttccggc
    16501 tggggacgat gcggaggaac attattaggg atgattgtga cccgcgtcag accaatcaaa
    16561 ttcgccagcg tttccacggg ttttagatga ccatagtgca ccggatcaaa ggtgccgcca
    16621 aacagagcct gtaaagattt catatcaacc gtcgataaat acgtccgcca ggggtttatg
    16681 gcacaacaga agagataacc cttccagctc tgcccacact gactgaccgt aatcttgttt
    16741 gagggtgagt tccgttcgtg tcaggagttg cacggcctga cgtaactgcg gctgacttaa
    16801 gcgatttaac gcctcgccca tcatgccccg gcggttctgc catacccgat gcttatcaaa
    16861 caacgcacgc agtggcgtat gggcagactg gcgtttcagg ttaaccagta acaacagttc
    16921 acgttgtaat gtgcgcaaca aaataaccgg ctcgctgcct tccagacgca gttgctgaag
    16981 aatatgcaac gcgcgcttac tttttcccat caacaaagca tcaacccaat gaaaaggggt
    17041 gaaatgcgcg gcatcattca ccgcctgttc aacgcgcggt aatgtcagtt tgccgtctgg
    17101 ccagagcagc gataaacgct ccagtgcctg agccagcgcc agcaggttac cttcataaca
    17161 gtagcagagc acctgattcg ccgcgtcatc cagttctaag ttgagctgtt ttgcgcgcac
    17221 agcaacccag cggggaagct gagcctgctc cggtgtctga caggtcacct gcacgctgcg
    17281 attcgcaaga gcagtaaacc aggcggcatt ttcttgcgct ttgcttaatt tattaccgcg
    17341 gacgatcaac agcaggtcgt catgcagaag tccggtgagt gtgagaagtt gttcattgat
    17401 cgccgcattc ggtccgtttt ctggtaacaa cagcaatagc gtttgtcgac tggcaaacag
    17461 actcatagcc tggcataacg aaaagatcgc attccagtca gtgttgggat caatggaaaa
    17521 agtgtggtgt tcttcgaatc cttgtgccgc agctacctgg cgaacagcgt cctggctttc
    17581 ctgcaataac agaggatcgt taccaagtaa aagatacgcc gcgcgcagcc cttcattgag
    17641 ctgcgcgcgg agttgttccg ggtacaaccg aatcatcagt tacccagcat ggtggagacg
    17701 cgcgcaggcg ttgccggagt atccgttgtg gtcgacgtct gttcttcgtc ggaacgaata
    17761 tccgcagcac ggatgcttgg cagcttacga atcagctgtt cggcagcacg gtcgtacatc
    17821 tcttttacga tcatgtcttg ttcgttatct ttcgctaacg ccatttgcgg gttatcgaag
    17881 aatgaacgga agactttggc gctaattggg tagatatcac ggccggggat caacacggtc
    17941 gcattcaccg tcatgatcat ctgatactct gctgtttgac cgttacggaa taccgatgcg
    18001 gtatctttcg cgatgctcac tttacccaaa cgcagggatg gaacgtcctt acgcgtggtt
    18061 tctttatcaa gcaactcgac accattcaga cgtaactggt tacgcaccgc acggcttaat
    18121 ggcccgttcg gatcgcctga gtccaggatc atgaccttca tagtggaagg aacctgcgtg
    18181 gtgtcacgca gatgccagcc acacccggcg gtgattaaca ccgccagaga taacaacaat
    18241 gttgccagat atcgcacgct tcctcccgcg cttagccaac gaccagattg aggagtttac
    18301 ctggtacgta aatcacttta cgtacagtaa cgccatcaag atattttgct accagatgtt
    18361 cctggccagc acgttcgcga acctgttctt ccgttgcgtc caccggaacg gtgattttgg
    18421 cacggacttt accgttaacc tgcaccacga ccagcgtgga gtcttccacc atcgcttttt
    18481 cgtcagcaac cggccacggc gcgttgtcga tatcgccttc gcctttgagt tcctgccaca
    18541 gcgtgaagca gatgtgcggg gtgaacgggt tgagcatacg gacaacggcc agcagtgctt
    18601 cctgcatcag agcgcgatcc tgctcgccat cggtcggtgc tttcgccagt ttgttcatca
    18661 gctccataat cgccgcaatt gcggtgttga aggtctgacg acggccgata tcatcggtca
    18721 ctttagcgat agttttatgc acatcgcgac gcagcgcttt ctgatcttca gtcagcgcat
    18781 caacgttcag tgccgcaaca tcaccttttg ctgtgtgctc gtacaccagt ttccagacac
    18841 gtttcaggaa gcggttagcc ccttccacac cggattcctg ccattcgaga gtcatatcag
    18901 ccggagaagc aaacatcata aacagacgaa cggtgtccgc gccgtaacgt tcaaccatca
    18961 cctgcgggtc gataccgttg ttcttcgact tggacatttt gctcatgccg gtataaacca
    19021 gttcatggcc tgccgcatct ttcgctttca cgatacggcc tttctcgtca cgttcaacga
    19081 tagcatcaac cggggaaacc cagttacgtt cgccgttttc gccaacatag tagaaggcat
    19141 ctgccagcac cataccctga cacagcaact gtttcgctgg ttcgtcagag ttcaccatgc
    19201 ctgcatcacg catcagtttg tggaagaagc ggaagtagag caggtgcata atggcgtgtt
    19261 cgataccacc aatgtagata tccaccggca gccagtagtt agccgcttcg gaatccagca
    19321 taccttcttt gtactctggg caagtgtagc gcgcatagta ccaggaggac tccataaagg
    19381 tgtcgaaagt gtcggtttca cgcagcgctg gcataccgtt aacggtagtt tttgcccact
    19441 ccggatctgc tttaatcggg ctggtaatgc cgtccatcac cacatcttcc ggcaggatca
    19501 ccggcagctg gtcgtccggg gttggcatta cggtaccgtc ttccagcgtc accatcggaa
    19561 tcggcgcgcc ccagtaacgc tgacgagaaa caccccagtc gcgcaggcgg tagttcactt
    19621 tacgctcgcc aacgcccatc gcagtcagtt tatcggcgat ggcgttgaag gccgcttcat
    19681 ggtcaagacc gttgaactcg ccagagttga acagcacgcc tttttcagtc agggcttgct
    19741 gagaaagatc tggctcagag ccgtcagccg ccaggataac cggtttgatg ttcaggccgt
    19801 atttagaggc aaactcgtag tcgcgctggt cgtgacccgg aaccgccata actgcgcccg
    19861 tgccgtactc catcaatacg aagtttgctg cccaaacggg aatttcttcg cccgttaatg
    19921 ggtgaacagc tttaaagcca gtatcgacgc cttttttctc cagcgtcgcc atttcagctt
    19981 cggcaacttt ggtgttacgg cattcgtcaa taaaggccgc cagttcagga ttattttccg
    20041 ccgctttctg cgccagcgga tgacccgcag ctaccgccag gtaggtacaa cccataaagg
    20101 tgtccgggcg ggtagtgtaa acggtcagcg tgttgtcata gtcgttaacg ttgaaggtga
    20161 tctccacgcc ttcggaacga ccgatccagt tacgctgcat ggttttaacg gtgtctggcc
    20221 agtgatccag tttatccaga tcgttgagca gctcgtcagc gtaagcagtg attttgataa
    20281 accactgcgg gatctcttta cgttcaactt tggtatcgca gcgccagcag cagccgtcga
    20341 taacttgttc gttcgccagt acggtctggt cgttcgggca ccagttgacc gcagaagtct
    20401 ttttatatac caggcctttt ttatacagct cggtgaagaa tttctgttcc caacggtagt
    20461 attccggcgt acaggttgcc agctcgcggc tccagtcata accaaagccc agcattttga
    20521 gctggttttt catatacgcg atgttgtcgt acgtccacgg tgccggagcg gtgttgtttt
    20581 tcaccgccgc gccttccgca ggcagaccaa acgcgtccca gccgatcggc tgcagaacgt
    20641 ttttgcccag catgcgctgg taacgggcga tcacgtcacc gatggtgtag ttacgtacgt
    20701 ggcccatgtg tagtcgacca gaaggatagg gaagcataga caggcagtaa tacttctctt
    20761 tgctctcgtc ttcggttact tcaaatgtgc gcttctcatc ccaatgaagc tgtactttgg
    20821 attctatctc ttccgggcgg tattgctctt gcatggcagc cagtggtcct gttttcaata
    20881 cggctacaaa tgtagcgttg aggtggtttt tcagatccgc atagcatagc ccaaacgtcc
    20941 gcgtcaaaac agcctttcgc gcactcgacg ttgaaatgat gcccggatta ttcgtacatt
    21001 aatttacaga gtttgtgggc gtattagcaa agcaaggaac aaagaacgtc tattattata
    21061 gtcagttaac gacccgggag atgaaacgat gaacaaggtt gctcaatatt accgtgaact
    21121 ggtcgcgtca ctgaacgaac gcctgcgcaa tggcgaacgt gatatcgacg cactggtgga
    21181 acaggcgcgc gagcgcgtaa taaaaacagg ggagttaacg cgaaccgagc tcgatgagct
    21241 gacgcgagct gtcagacgtg acctggaaga gttcgccatg agctatgaag agagcctgaa
    21301 agaagaatct gacagcgtct ttatgcgggt gattaaagaa agcttgtggc aggagctggc
    21361 agacatcacc gataaaacgc agcttgaatg gcgcgaagtt ttccaggacc tcaatcatca
    21421 tggggtttat cacagcggag aagtggtcgg gctgggaaat ctggtctgcg agaaatgtca
    21481 cttccatctc ccgatctaca caccggaagt gctgacgcta tgcccgaaat gtggttatga
    21541 ccagttccag agacgcccgt ttgagccgta atagtctgat ttcgcatagc aataaagaga
    21601 atatgggcag aatgaaattc cccggccctt gtttttattt ctacagtaaa gcttcagtct
    21661 cacgaatttc ctgactgaca tctctggttc cggatgactg aactttacga tacaggtcga
    21721 gagcctgtcg gagatcgaca ggtcttcctt ctccgtagtg atacataatg gccaggttat
    21781 accaggcagt ggcgtcattg cattcagcgg ctttcgtaaa ccattcaaag gcagcctgat
    21841 aatctttctc aacgccttgt ccgtgtttat acatataacc gatgttgacg taagcgccga
    21901 cgctttcctg agcggcagcc ttcagatacc agtacatcgc ttgcttatag tcctggtcca
    21961 ccccttcgcc agcgttataa tcccatgcaa tttgaaactg ggcgtgccga ttaccctgct
    22021 gtgcgctttt caaataccag aatgcggcga gtgtcttgtt ttgagcaacg ccttctccgt
    22081 ctttataaag atcggccaga ttgtcttgtg cgtaactatg gccttgtaat gcagcttgtt
    22141 tgtaccagaa aaatgccagc gtatagtctt gtgcgactcc gttgccattt ctgtacatcc
    22201 atccaagatt attttgcgca taagacattc cgctctccgc ggctatctga taccaggtaa
    22261 ccgcttcttt atagttgggg tcttctccac gatccagtat ccagccaagg gcgttggaag
    22321 cgaaagtatg gccttgtagg gcagcttttt tcagccagaa gatggcttgc tcattatctt
    22381 tatgacggct tttatcctcg gtatactttt gccctaagga atactgtgct tcacaatgac
    22441 cttgctcagc ggccagcttc agccagtaaa aggccttttc gtcgtccgga gagtcaattg
    22501 cgctatcgcg attataataa aacccaacaa tatattgagc ctcgcaatcg cctttttcag
    22561 cacgttcgat aatctcatct attgataaat tatcgcagca acttgacgta aaaattatta
    22621 tcatttcctt atggttgtag ataaaatata ttatattttt atgtgttcca tttttgcgta
    22681 agccttattt tatgcgtatt atgttgcgtc agaaaagtat aaataactga gtctcgaaat
    22741 gtagtttgca ttcattaaat gaatgtttaa ggataaatta tatggacatg gggtcgcaaa
    22801 aaatattgtt tgcactttcc actccgatgg aaatacgcaa tgaatgctgt ttaccttcgc
    22861 attcatcccc caaaatgtat ttaggtacat gcttttttga cctttcttcc tcatggggaa
    22921 ttgatgaccg tgatgacctg ctgcgcacaa ttcaccgaat gatcgagaac ggtcatgccg
    22981 ctcggttggc agggttttat caccgctggt ttcgttattc tccatgtgaa tggcgtgact
    23041 atcttgctga attaaatgag caaggtcagg cttatgcgca atttgtcgcc agcacagccg
    23101 aatgctgtgg tgagggagga attaaagcat gggattatgt ccggatgggt tttttaagtc
    23161 gaatgggcgt acttaataac tggttgagtg aagaagaaag tctatggatc ctgtcgcgta
    23221 ttcatctacg ggctctacgt tattatagta actggcggca atactttgcc ggttatacct
    23281 ttggtcggca atattggcag tctcccgaag atgatcatct gcctttatta cgcgaattct
    23341 tagcgcgtaa agaatacgac gattccggca atgatatgtt ttatcaatta tttgccagtg
    23401 atgatgcgta ttaccctacc ttgtcctggc aaccactggc tgactacccc acatgcccgg
    23461 aaacgcttaa ggatatgagc gacttatgaa aaattgctgg aagatcctcg atatagagga
    23521 aacgactgac gtcgatatta tccgccgcgc ttatctggcg ctgttaccgt cctttcatcc
    23581 agaaaccgat ccgcagggtt ttaaacaact tcgtcaggcg tatgaggagg cgctacggat
    23641 tgcgcagtcg cctgctaaat ctgtttggca accagaagaa tatgaggtag cagaacatga
    23701 aattctgctc gcctttcgcg cgttacttgc ctctgatagc gaacgttttc tgccctccgc
    23761 ctggcagcga ttcattcagc aattaaatta ttgctcgatg gaagagattg atgaattacg
    23821 ctggtcgctg tgcacaatcg ccatgaacac tgcccattta tccttcgagt gcgtggtgtt
    23881 attagcagaa agattgcggt ggttgcagga ggaaaacgtc ggggaaatag acgaagaaga
    23941 actggaatcc tttttatatg ccattgcgaa ggggaatgtt tttaacttcc agaccattct
    24001 gcatctgccc gttgccgtgc aaaatgacac catcgatttt taccaaatgt tcgctcggat
    24061 ttggtcatcg catccagaat ggctgacatt gtatttagcg caacatcgcg cagtgattat
    24121 ccccgatgat gcaaaactgc acagaaattt actccgctgg tatagcgcaa gccgcctggg
    24181 aatccccgaa ctcctggatt acgcccggtc gtggcgggaa gctgaacctg ataatgaaga
    24241 tgcgcgttat tatgaatacg cacaacgcgt ctattgtgga gaaggcgaaa gcctgctggc
    24301 agaactttgt tactactggc gcgagtatcc ctccacccag gcggatgctt taatgttgca
    24361 atggtgccgt cagcatcggg tcgattatta cccattagtg gtgatgatga ttgaagcgcg
    24421 tgatctggtt aacgatcagg gaaaaccgct actttatgtc cccggcgaca gcgcccgtac
    24481 gcgttttcat ttatacgaaa tactcagcga tgaaaaactc tctgcgctgg ggcgttcact
    24541 ggtcgagatg gttttgcaca aaggacgtaa gccgcggatc tcactcacgc gtgatacaga
    24601 acatccctta tggccattat atttagttgc caaacaatta gtgcaggcca gccaaccgac
    24661 agaagaatca ttaatgccga ttgtgagccg ccttgatgca gaagatcgtt gtccactgga
    24721 agcattaatt attcgtcgat tattaattca ggcggcgaat tttaccggtc aggaaaccgt
    24781 cgagccggag ccgcaaccgc agccaatgcc cgttgacgat ggcgggctgg gctgtctggg
    24841 cgtcatcaaa attattttct acatttttat ctttgccggt ttgataggga aaatactcca
    24901 tctcttcggg tgacagtcga cgttaatgtg cgggaagtcc ggcaagtaat ctttccgcat
    24961 ttttgtaatg ttgtttggcg gccagtttta accatttttg agattcataa tggttaacat
    25021 taataccagt gccatttctg tagatcaaac ccaaagtata ttgagcatga gcataccctt
    25081 gttcagctgc ttccttatac cagagaattg ctttacgggt atcgggtttg actcccaggc
    25141 ctttcaggta gatccacccc agtttgcttt gtgcgcgcgg attttcctgt tctgctgatt
    25201 gctcatacca gtatctcgcc tgtgtataat ccagtggttt accgtcgcta ccatgaagat
    25261 aaaattcgcc aaggcgatat tgcgatgttg agtgaccatg aatcgcggaa agctccgtgg
    25321 ctgccgtggt taacggtaga ctgcctggtg atcttaaata atcaaaggca taattcacca
    25381 tggctttatc acgcaacgtc gttttcttgg gttggctaat acaggtaatt gctgcaacta
    25441 taaa
//
LOCUS       DAETPZ010000054        25084 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.054, whole genome shotgun sequence.
ACCESSION   DAETPZ010000054 DAETPZ010000000
VERSION     DAETPZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.054"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            145..297
                     /gene="hokA"
                     /locus_tag="K0W59_002516"
     CDS             145..297
                     /gene="hokA"
                     /locus_tag="K0W59_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HBI3023511.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(484..696)
                     /gene="cspA"
                     /locus_tag="K0W59_002517"
     CDS             complement(484..696)
                     /gene="cspA"
                     /locus_tag="K0W59_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HBI3023512.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNYGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(977..1267)
                     /locus_tag="K0W59_002518"
     CDS             complement(977..1267)
                     /locus_tag="K0W59_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HBI3023513.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIERLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLMK"
     gene            1701..2411
                     /locus_tag="K0W59_002519"
     CDS             1701..2411
                     /locus_tag="K0W59_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HBI3023514.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2461..3435)
                     /gene="ghrB"
                     /locus_tag="K0W59_002520"
     CDS             complement(2461..3435)
                     /gene="ghrB"
                     /locus_tag="K0W59_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HBI3023515.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3539..4198)
                     /locus_tag="K0W59_002521"
     CDS             complement(3539..4198)
                     /locus_tag="K0W59_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HBI3023516.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4351..6684
                     /gene="bisC"
                     /locus_tag="K0W59_002522"
     CDS             4351..6684
                     /gene="bisC"
                     /locus_tag="K0W59_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HBI3023517.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLAHCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6653..7093)
                     /locus_tag="K0W59_002523"
     CDS             complement(6653..7093)
                     /locus_tag="K0W59_002523"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBI3023518.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7090..7653)
                     /gene="tag"
                     /locus_tag="K0W59_002524"
     CDS             complement(7090..7653)
                     /gene="tag"
                     /locus_tag="K0W59_002524"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBI3023519.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKCGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7811..8509
                     /locus_tag="K0W59_002525"
     CDS             7811..8509
                     /locus_tag="K0W59_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBI3023520.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDSTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8738..9946
                     /locus_tag="K0W59_002526"
     CDS             8738..9946
                     /locus_tag="K0W59_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3023521.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10270..11961
                     /gene="eptB"
                     /locus_tag="K0W59_002527"
     CDS             10270..11961
                     /gene="eptB"
                     /locus_tag="K0W59_002527"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HBI3023522.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12053..12129
                     /locus_tag="K0W59_002528"
     tRNA            12053..12129
                     /locus_tag="K0W59_002528"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12087..12089,aa:Pro,seq:cgg)
     gene            12873..14480
                     /gene="dppA"
                     /locus_tag="K0W59_002529"
     CDS             12873..14480
                     /gene="dppA"
                     /locus_tag="K0W59_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HBI3023523.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14788..15807
                     /gene="dppB"
                     /locus_tag="K0W59_002530"
     CDS             14788..15807
                     /gene="dppB"
                     /locus_tag="K0W59_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HBI3023524.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15817..16719
                     /gene="dppC"
                     /locus_tag="K0W59_002531"
     CDS             15817..16719
                     /gene="dppC"
                     /locus_tag="K0W59_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HBI3023525.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16730..17713
                     /gene="dppD"
                     /locus_tag="K0W59_002532"
     CDS             16730..17713
                     /gene="dppD"
                     /locus_tag="K0W59_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBI3023526.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17710..18714
                     /gene="dppF"
                     /locus_tag="K0W59_002533"
     CDS             17710..18714
                     /gene="dppF"
                     /locus_tag="K0W59_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HBI3023527.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18744..20015)
                     /locus_tag="K0W59_002534"
     CDS             complement(18744..20015)
                     /locus_tag="K0W59_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBI3023528.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20491..20598
                     /locus_tag="K0W59_002535"
     CDS             20491..20598
                     /locus_tag="K0W59_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBI3023529.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            20974..21081
                     /gene="ldrD"
                     /locus_tag="K0W59_002536"
     CDS             20974..21081
                     /gene="ldrD"
                     /locus_tag="K0W59_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HBI3023530.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21457..21564
                     /locus_tag="K0W59_002537"
     CDS             21457..21564
                     /locus_tag="K0W59_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBI3023531.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21940..22047
                     /locus_tag="K0W59_002538"
     CDS             21940..22047
                     /locus_tag="K0W59_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBI3023532.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(22134..23813)
                     /gene="bcsG"
                     /locus_tag="K0W59_002539"
     CDS             complement(22134..23813)
                     /gene="bcsG"
                     /locus_tag="K0W59_002539"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HBI3023533.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLLGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(23810..24001)
                     /gene="bcsF"
                     /locus_tag="K0W59_002540"
     CDS             complement(23810..24001)
                     /gene="bcsF"
                     /locus_tag="K0W59_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HBI3023534.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(23998..>25084)
                     /gene="bcsE"
                     /locus_tag="K0W59_002541"
     CDS             complement(23998..>25084)
                     /gene="bcsE"
                     /locus_tag="K0W59_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HBI3023535.1"
                     /translation="EEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQN
                     GIWTLVQSEEAEIQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAA
                     TVVFSLQQNAQIEPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVI
                     PWNAPLSRCLTMIESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMM
                     NNPLLPAHGKGVLVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRIND
                     LDTALNHIFPLPTGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVI
                     NAEHDGRHWRRIPEPMRLLDDAVERSS"
BASE COUNT         6057 a         6223 c         6657 g         6147 t
ORIGIN      
        1 tccaattgaa tatcggaatc agacctatat gcctcgtgtt taactgtcca actttttggg
       61 gtcagtacaa agtgaggcta ccctatgcct gaacaccata aggtgtgcct cttactcgtt
      121 ggaaatcaac acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg
      181 atttgtttca cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat
      241 attaaacagg agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag
      301 tcttcggcgg gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccac
      361 atttattctt cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga
      421 aaaaaaataa aaaaatcccc gcaaaatggc agggatctta gattctgtgc ttttaagcag
      481 agattacagg ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa
      541 ggacactttc tgaccttcgt ccagagattt gtaaccatag ttctggatag cagagaagtg
      601 tacgaacaca tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt
      661 gaaccatttt acgataccag tcattttacc ggacatagtg tattaccttt aataattaag
      721 tgtgcctttc ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact
      781 acgaggggta tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc
      841 tgtacgtcaa accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt
      901 tttcatattt atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa
      961 taaagtggat aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca
     1021 aacgcatcaa ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta
     1081 ccatggcgac tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt
     1141 ttcgtaaccg ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg
     1201 tttcatcttt aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat
     1261 attccattga aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta
     1321 agaatagtcg ggaaaagcag acacagcggg gttaagtcag ggcatgctct ggctatgatt
     1381 cgattcagcc tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata
     1441 tatctcttat cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta
     1501 aatctctggc tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt
     1561 aaccttgttt cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc
     1621 aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt
     1681 tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc
     1741 ttgcggcgtt attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc
     1801 gtaaagcgtt tatcgacttc ctgcagaata cagtgatgcg tagcggtgaa cgtctaccaa
     1861 ccctgactgc cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt
     1921 atggttattc tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca
     1981 gcgttaacgc aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa
     2041 tgaatggttc gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg
     2101 ctgcgcactc tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca
     2161 ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt
     2221 tcacgcaaca actggtgatg gttggagact acattgctca gcaaggtact caggtgagct
     2281 ttgtcgcaaa tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga
     2341 ttgcgccatt accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg
     2401 caacccaata aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcggc
     2461 ttagtccgcg acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat
     2521 caaattatcc acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat
     2581 atgcggtact gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc
     2641 ttgttcgaag acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag
     2701 tgcattttcg tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat
     2761 tttggcgaat tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag
     2821 gcaaacgaaa tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg
     2881 ttcttctgct tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa
     2941 gtgcgcacgt tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt
     3001 atggtgaacg tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt
     3061 tacccgttct gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt
     3121 atcggcgacg gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt
     3181 aagcgcatcg acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt
     3241 cggcattttt tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc
     3301 ttctgcaaaa attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg
     3361 aacggtgaaa tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat
     3421 aacggacggc ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata
     3481 tgatgaagga aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt
     3541 acagcgggct taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt
     3601 tgctggcgat tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc
     3661 cctgggtgat caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca
     3721 ggtcgtgacc ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact
     3781 ctttcagcac cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggcgcgc
     3841 tgctgctgtc gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg
     3901 ttacgctaac accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt
     3961 aataacccac gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac
     4021 cgcgatcttt cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac
     4081 ctgcgccgat agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc
     4141 cagataccgc cagagcgcca ctcactacgg cggcaataag ataaacacgt ttcttcattg
     4201 ttaatcctta ataacctttt tattctttgc cacgggttcc gtggcgggag attatgccgc
     4261 gtgaacatga agattattcc tgggaatact cggaaatttg taagtaatat ttaactgctc
     4321 aatacatcta acctttcagg agtcttcggt ttggccaact catcctcacg atattccgtt
     4381 ctgactgccg cccactgggg gcccatgctg gttgaaaccg acggcgaaac cgtgtttagc
     4441 tcgcgtggcg cgttagccac aggaatggaa aactccttgc agagcgcggt tcgcgaccag
     4501 gttcacagca atacgcgggt acgatttcca atggtgcgca aaggctttct cgcgtcaccg
     4561 gaaaacccgc aaggcattcg tgggcaggat gaatttgttc gcgtgagttg ggatgaggcg
     4621 ctggatctta ttcaccaaca acataaacgc attcgtgagg cttatggtcc ggcatcgatt
     4681 tttgctggtt cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc gacattatta
     4741 caacgctata tggcgctggc aggcggttat accgggcatc tgggggatta ttcgaccggt
     4801 gcggcacagg cgatcatgcc gtatgtcgta ggtggcagcg aagtttatca acagcaaacc
     4861 agttggccgc tggtgctgga acatagcgat gtcgtggtgt tgtggagtgc taacccactc
     4921 aatacgctga aaattgcgtg gaatgcatcc gatgagcagg ggctttctta cttttccgca
     4981 ctgcgtgaca gtgggaaaaa gctgatctgc attgatccaa tgcgatcgga aaccgtcgat
     5041 ttctttggcg ataaaatgga gtgggtggca ccgcacatgg gcaccgatgt tgcgctgatg
     5101 ctggggatcg cccatacgct ggtggaaaat ggttggcacg acgaagcgtt tctggcgcat
     5161 tgcaccacag gttatgccgt cttcgcctct tatttgctgg gcgagagtga cggaatagcg
     5221 aaaaacgccg aatgggcggc agagatttgt ggtgttggcg cagcgaaaat ccgcgagctg
     5281 gcggctattt tccaccaaaa taccaccatg ctgatggcag gctggggaat gcagcgccaa
     5341 cagtttggtg agcaaaaaca ctggatgatc gtcacgctgg cagcaatgtt ggggcaaatc
     5401 ggcacacccg gcggcggttt tggtctttct taccattttg ccaatggtgg taaccccacg
     5461 cggcgttctg cggtgctctc ttccatgcag ggcagcttgc cgggtggctg cgatgcggtg
     5521 gataaaatcc ctgttgcccg cattgttgaa gcactggaaa accctggtgg cgcatatcaa
     5581 cacaacggta tgaaccgaca tttcccggat attcgtttta tctggtgggc gggcggtgcc
     5641 aactttactc atcatcagga taccaatcgt ctgatccgtg cctggcaaaa accggagctg
     5701 gtggtgatct ctgaatgctt ctggacggcg gcggcgaaac acgcggatat cgttctgcct
     5761 gcgactacct cttttgagcg taatgatctc accatgaccg gtgattacag taatcagcat
     5821 ctggtgccga tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga ttttgatgtt
     5881 tttgccgagt tgagtgaacg ttgggagaag ggcggctatg cgcggtttac ggaaggaaaa
     5941 agtcagctgc aatggctgga aacgttttat aacgttgccc ggcagcgcgg ggcaagccag
     6001 caggttgaat tgccgccatt tgctgagttc tggcaagcca accagttaat tgagatgccg
     6061 gaaaacccgg acagcgagcg gtttattcgc ttcgctgatt tttgccgcga tccgctggcg
     6121 catccgttaa aaaccgccag cggcaagatt gaaatcttct cacagcgtat tgccgattac
     6181 ggttacccgg attgccctgg gcatccaatg tggctggagc cggacgaatg gcagggcaat
     6241 gccgaaccag aacagttgca ggtactttct gcccatccgg cgcaccgcct gcacagccag
     6301 ctgaattaca gttctctgcg cgaattgtac gcggtagcaa accgtgagcc tgtcaccatt
     6361 catcctgacg atgctcaggc gcgcggcata accgaagggg atatggtgcg ggtgtggaac
     6421 tcccgtgggc agatccttgc tggagcggtc attagcgagg gaattaaacc tggcgtgatt
     6481 tgcattcacg aaggggcatg gccggatctg gatttaaccg ctgacggtat ttgtaaaaac
     6541 ggcgcagtga acgtgctgac caaagatctc cccagctcgc ggctggggaa tggctgtgcg
     6601 ggtaatacgg cgctggcatg gctggaaaaa tacaacggtc cggaactgac acttacagcg
     6661 tttgaaccac cggccagctc ataatccagg tgggtagttg ggtttcatcc tgccatgcgc
     6721 aatcgacaat gtgaaaaccc tgcgcacggt aaaaatctat cgccggttga tttttttgat
     6781 aaacctccag catcagatgg ggatagcgct gctgcacata ctgcatcagc gccttaccaa
     6841 taccgcgtct gacggccttc ggtgcgacaa acattgctgc cagaaatcgg ccttccataa
     6901 tgctgacaaa accgagaagc ttaccgtcct cttcccagac ccagttttgc gcgttggcaa
     6961 gataggcatc ccgcaccagc ggaatgcagt cacgccagta attcgctttt ataaagggat
     7021 gcccccaggt tgtactttcc agccacagtt cgaggatcgc ggggagttct gaacgttgcg
     7081 cttcccgaat catggtttat ttcccggata gcaacagcag ccaaccacat gatcattcac
     7141 cagcccacat gcctgcataa aggagtaaca gattgtggtg ccgacaaact taaaaccaca
     7201 ttttttcagt gccttagata gggcgtcgga ggcggacgta gatgtgggaa tttcgctcaa
     7261 cgttgtggct tgtgtcacct gtggctgatg atttacaaac gaccagacaa agtcgggaaa
     7321 cggttcgccg ttctgttcca tttgcaggta cgcccgcgca ttaccaataa ttgcctgaat
     7381 tttccctcga tggcggataa tcccggcgtc ctgtaccagt ctttcgacat cctcttcctg
     7441 cattgctgcg accttcaccg gatcgaactg atgaaagcag gcgcgatagt tttcgcgttt
     7501 tttgaggacg gtgatccacg ataacccagc ctgctgccct tcaaggcaga tcatttcgaa
     7561 cagtttttta ctgtcagttt caggcacgcc ccactcatta tcatggtagg caatataaag
     7621 cgggccctga ctcacccagc cgcaacgttc catactttcc ctcgctatgt acccgatcgt
     7681 aaaaatttca gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta atacagggct
     7741 actatgcccg atatccttca tacaatgaca ataatattgc cgaattcggc tcttccctgg
     7801 agtcgtgtta atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc tgctggcgag
     7861 cgtggtaatc agtgcctttt ttctcaacac agcttacgcc tggcaacaag aatatatcgt
     7921 tgatacgcaa cccggacatt ccacagagcg ttacacctgg gatagtgatc atcaacctga
     7981 ttacaacgat attttgtcgc aacgtattca aagtagccaa agggcgctgg gactggaagt
     8041 caatctggcg gaagaaactc ctgtggatgt gaccagcagt atgagtatgg gctggaattt
     8101 tcctttatat gaacaggtta caaccggccc ggtcgcggca ttacattacg atagcacaac
     8161 cacctcgatg tataacgagt ttggcgacag tactaccacg ctgaccgatc cgttatggca
     8221 tgccagcgtg agtagcttag gctggcgtgt tgactcccgg cttggcgatc tccgaccctg
     8281 ggcgcaaatc agctataacc agcaatttgg cgagaatatc tggaaggcgc aatcaggcct
     8341 gagccggatg acggcgacaa accagaacgg caactggctg gatgtcaccg taggcgctga
     8401 tatgttgctc aatcaaaata ttgccgccta tgctgcgcta actcaggcag aaaataccac
     8461 taataatagc gactatctgt atacgatggg ggttagcgcc agattttaac gtaacagtca
     8521 caattgaaac cattgaataa caacagttgt ggcgatagtg ggtgctaact taccaaataa
     8581 taaatttggt gaataattgt cgcgtcattc attcctgaac taaggcattt cattccgttc
     8641 tgatggcatt tcatgccgtt tttccccaag cataaaatgc acttcgttat ggttgtcggc
     8701 agagattttt cctttttatt actgcaggaa tactgccatg acaccttcaa attatcagcg
     8761 tacccgctgg ctgacactca tcggtactat cattacccag tttgcgctgg ggtcggttta
     8821 tacctggagc ctgtttaatg gcgcgctttc tgccaagctg gatgcgccgg taagccaggt
     8881 cgctttctct ttcggcttgt taagtctggg gctggcaatt tcgtcttctg ttgcgggcaa
     8941 attgcaggaa cgttttggcg ttaaacgcgt caccgtggct tccggcattt tgctgggatt
     9001 aggcttcttc ctgaccgcgc attccaacaa cctgatgatg ctgtggttaa gcgccggtgt
     9061 gctggtcggt ctggcggatg gcgcgggtta cctgctgacg ctctctaact gcgtgaagtg
     9121 gttcccggag cgtaaaggtc tgatctccgc gttcgctatc ggttcttatg gtctgggtag
     9181 cctgggtttc aaatttatcg acacgcagct gctcgaaacg gtgggtctgg aaaaaacctt
     9241 tgtgatttgg ggagcgattg cgctggtgat gattgttttc ggcgcaacgt taatgaaaga
     9301 cgcaccaaaa caggaagtga aaaccagcaa tggtgtggtg gagaaagatt acacgctggc
     9361 agagtcgatg cgtaaaccgc agtactggat gttagcggta atgttcctga ctgcctgcat
     9421 gagcggcctg tacgtgattg gggtagcgaa agatatcgcc caaagtctgg cacaccttga
     9481 tgtggtttcc gcagccaatg cagtaacggt tatttccatc gctaaccttt caggtcgtct
     9541 ggtgctgggc attctgtctg acaaaattgc ccgtatccgt gttatcacca ttggtcaggt
     9601 gatttcgctg gtgggtatgg cggccctgct gtttgcacca ttaaatgcag tgacgttctt
     9661 tgcagcgatt gcctgcgtgg catttaactt tggcggcact attaccgtct ttccatcact
     9721 ggtcagtgag ttcttcggcc tcaataacct ggcgaaaaac tacggtgtga tttacctcgg
     9781 tttcggtatc ggtagcattt ttggttcgat tatcgcctca ctgtttggcg gtttctatgt
     9841 gaccttctac gtgattttcg ccctgctgat tctgtcattg gcgctttcta cgacgattcg
     9901 tcagccagag cagaaaatgt tgcgtgaggc gcatggctcc ctttaatgct cagccgcgag
     9961 ctactgggct tcagcttccg tgtgcatcaa acgcatcaac gaaatgtata aacaatgact
    10021 cgtaaaattt gggccagtta actggccctt tttactgctt tttgtaacgc taaagtctct
    10081 tttcaaactt gcatttttgt aaatttgtgc ttcatgcaca ctctttcccc acactttttc
    10141 gctttgctgt ggtctactta ttcgcgcgtg tagattttac ttatctgact acctccgcac
    10201 ttttcccctg ccgggcctga aaagccgcta agcagggtgt tatcacctgt ttgtccaggg
    10261 tttgtttgca tgagatacat caaatcgatt acacagcaga agctgagctt tttgcttgca
    10321 atctatattg gcctttttat gaatggcgcg gttttttatc gccgcttcgg cagctatgcg
    10381 cacgatttta ccgtctggaa aggcgtttct gctgttgttg aactggccgc caccgtactg
    10441 gtgaccttct ttttactacg tcttctatcg ctgtttggcc gccgcagctg gcgtattctg
    10501 gcgtcgctgg tggtgctctt ttccgcaggt gccagctatt acatgacctt ccttaatgtg
    10561 gtcattggtt atggcatcat cgcttccgtc atgaccaccg atatcgacct gtcaaaagaa
    10621 gttgttggtc tgaactttat tctctggtta atcgccgtta gtgcattgcc tcttatcctt
    10681 atctggaata accgctgtcg ctacaccttg ctccgacaac tgcgaacccc agggcagcgt
    10741 attcgcagcc tggtggtcgt cgtactggcg ggtattatgg tttgggcacc gattcgtttg
    10801 ctggatatcc agcagaagaa agtggagagg gcgaccggcg ttgatttgcc gagttatggc
    10861 ggtgtcgtag cgaactctta tctgccatca aactggcttt ctgcgttggg gctgtatgcc
    10921 tgggcgcggg tcgatgaatc ttccgataat aattcattgc ttaatccggc gaagaagttt
    10981 acataccagg ctccacaaaa cgttgatgat acttatgtcg tgtttatcat cggtgaaacc
    11041 acgcgttggg accatatggg tattttcggc tatgagcgta ataccacgcc gaaactggcc
    11101 caggagaaaa atctggcggc gttccgtggt tactcctgtg ataccgcaac caaactctca
    11161 ctgcgttgca tgtttgtacg tcaggggggc gcggaagata atccgcagcg cacattaaaa
    11221 gaacagaaca ttttcgcggt tctgaagcag ttaggattca gttctgacct ctacgctatg
    11281 cagagcgaaa tgtggttcta cagcaacacg atggcagaca acattgctta tcgtgagcag
    11341 attggtgcgg agccacgtaa tcgtggcaag ccggtagatg atatgttgct ggtagacgaa
    11401 atgcagcaat cgctagggcg caacccggat ggtaagcatc tgatcattct gcataccaaa
    11461 ggttcgcatt ttaactacac ccagcgttat ccgcgtagct tcgcgcagtg gaagccggaa
    11521 tgtattggtg ttgatagcgg ctgtaccaaa gcgcagatga tcaactccta tgacaactcg
    11581 gtgacctatg tggatcactt tatctccagc gtgattgatc aggttcgcga taagaaagcg
    11641 attgtgttct acgcagctga ccacggtgag tcaattaatg aacgcgagca cctgcacggc
    11701 acgccgcgtg aactggcacc gccggagcag ttccgcgtac cgatgatggt ctggatgtca
    11761 gataaatatc tggaaaatcc ggccaatgcg caggcgtttg cgcagctgaa aaaagaagcc
    11821 gacatgaaag tgccacgccg tcacgtagag ctgtacgaca ccatcatggg ttgtcttggc
    11881 tatacttcac cggatggtgg aattaacgaa aacaacaact ggtgtcacat cccgcagaca
    11941 aaagtggcag cggctaacta agcggcctgc tgactttctc gccgatcaaa aggcattttg
    12001 ctattaaggg attgacgagg gcgtatctgc gcagtaagat gcgctccgca ttcggtgatt
    12061 ggcgcagcct ggtagcgcac ttcgttcggg acgaaggggt cggaggttcg aatcctctat
    12121 caccgaccaa attcgaaaag cctgctcagc gagcaggttt ttttgcattt gtagctcatg
    12181 aggatgagaa cctccggggg cagggaggct cgactcgacg taggcctgat aagacgcgcc
    12241 agcgtcgcat caggcaacgg caccgcactt attgtcggat gcggcgcgac cgccttattc
    12301 gaccaacaca ccacacattt tcctgccaac tttgcgcccc atatctcact atccattaac
    12361 ggatttgtga cagaaaccat tgtctttttt tatatttgct taaatctttt ttgcgttact
    12421 tatggctggc gctggcattt tccgctgtgc gttttagcgt tcatggtatt aatcgctcag
    12481 atatccacct tttcctcaga tttttttcat taaatgcata aaacttaatc accaaatttt
    12541 gctaaatatt aagctgattg ttctggttat gttggagtag cacaaatttc tctgctgcaa
    12601 atagctgttt gaagtttttt taatctttgt ttgtggattc tcaccgttgg tgtaaatccc
    12661 ggttggctgt attgacgttt tcacattctg ttgacagatt gtaggtcacg aggggcattt
    12721 tatggaggat ccgcactgtt acactgatgt taattagtac ggcatcccca cctcataacg
    12781 ttgacccgac cgggcaaaaa acaaaaaagg tcaggcagcg acaacccact gcaaagggtt
    12841 aaaacaacaa acatcacaat tggagcagaa taatgcgtat ttccttgaaa aagtcaggga
    12901 tgctgaagct tggtctcagc ctggtggcta tgaccgtcgc agcaagtgtt caggctaaaa
    12961 ctctggttta ttgctcagaa ggatctccgg aagggtttaa cccgcagctg tttacctccg
    13021 gcaccaccta tgacgcctct tccgtcccgc tttataaccg cttggttgaa tttaaaatcg
    13081 gcaccaccga agtgatcccg ggcctcgctg aaaagtggga agtcagcgaa gacggtaaaa
    13141 cctatacctt ccatctgcgt aaaggtgtga agtggcacga caataaagaa ttcaaaccga
    13201 cgcgcgaaat gaacgccgat gacgtggtgt tctcgttcga tcgtcagaaa aacgcgcaaa
    13261 acccgtacca taaagtttct ggcggcagct acgaatactt cgaaggtatg ggcttgccgg
    13321 agctgatcag cgaagtgaaa aaggtggacg ataacaccgt tcagtttgtg ctgactcgac
    13381 cggaagcgcc gttcctcgct gacctggcaa tggacttcgc ctctattctg tcaaaagaat
    13441 atgctgacgc gatgatgaaa gccggtacac cggaaaaact ggacctcaac ccaatcggaa
    13501 ccggtccgtt ccagttacag cagtaccaaa aagattcccg tatccgctac aaagcgtttg
    13561 atggctactg gggcaccaaa ccgcagatcg atacgctggt cttctctatt acccctgatg
    13621 cttccgtgcg ttacgcgaaa ttgcagaaga atgaatgcca ggtgatgccg tacccgaacc
    13681 cggcagacat cgctcgcatg aagcaggata aatccatcaa cctgatggaa atgccggggc
    13741 tgaacgtcgg ctacctctcg tataacgtgc agaaaaaacc gctggatgac gtgaaagttc
    13801 gccaggcact gacctacgcg gtgaacaaag acgcgatcat caaagcggtt tatcagggcg
    13861 cgggtgtatc agcgaaaaac ctgatcccgc caaccatgtg gggctataac gacgacgttc
    13921 aggattacac ctacgatcct gaaaaagcga aagccttgct gaaagaagcg ggtctggaaa
    13981 aaggtttctc catcgacctg tgggcgatgc cggtacaacg tccgtataac ccgaacgctc
    14041 gccgcatggc ggagatgatt caggcagact gggcaaaagt gggcgtgcag gccaaaatcg
    14101 tcacctacga atggggtgag tacctcaagc gtgcgaaaga tggcgaacat cagacggtaa
    14161 tgatgggctg gactggcgat aacggggacc cggataactt cttcgccacc ctgttcagct
    14221 gcgccgcctc tgaacaaggt tctaactact caaaatggtg ctacaaaccg tttgaagatc
    14281 tgattcaacc ggcgcgtgct accgacgacc ataacaaacg cgttgaactg tacaagcaag
    14341 cgcaggtggt gatgcacgat caggctccgg cactgatcat cgctcactcc accgtgtttg
    14401 aaccggtacg caaagaagtc aaaggctatg tggttgatcc attaggcaaa catcacttcg
    14461 aaaacgtctc tatcgaataa ttaaaagcca tacaagactg atggcaaagg caaaactgcc
    14521 tgatgtgctc cgcttatcag gcctacgaaa attctgcaat gtattgaatt tgcacgattt
    14581 tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa caaagcgcac tttgtcaaca
    14641 atctgtatac ccggtggcgc tgtgcctctg cgtgcagcgc catccggcag caatacttca
    14701 ttctctgtcc gacaagggcg ggaattgatt tgtgagcaat acagacacgc agttccaggc
    14761 tgcgggtcac tatagagaat ccgggttatg ttgcagttta ttctccgacg tttgggactc
    14821 gtcatcccca cgtttatcgg tattaccctt ctcacatttg cctttgtcca catgatcccg
    14881 ggcgatccgg tgatgatcat ggcaggcgaa cgtgggatct cccctgagcg tcacgtgcag
    14941 ctgctggctg aactcggctt agataaaccg atgtggcagc agtatctcca ttacatttgg
    15001 ggcgtaatgc acggcgatct aggcatttca atgaaaagcc gaattccagt atgggaagag
    15061 ttcgtgccgc gctttcaggc cacgctggaa cttggcgtct gcgcgatgat ttttgctacg
    15121 gcagtcggta ttccggttgg tgtgctggct gcggttaaac gcggttccat tttcgatcac
    15181 actgcagttg gcctggcgct gaccggctac tcgatgccga tcttctggtg gggcatgatg
    15241 ctgatcatgc tggtttcggt gcactggaac ctgacgcccg tctccggtcg cgtgagcgat
    15301 atggtgttcc tcgatgactc caatccgtta accggtttta tgctaatcga caccgccatc
    15361 tggggtgaag acggcaactt tatcgatgcc gtcgcccata tgatcttgcc cgccattgtg
    15421 ctgggtacta ttccgctggc ggtcattgtg cgtatgacac gctcctcgat gctggaagtg
    15481 ctgggcgagg attacatccg caccgcgcgc gccaaagggc taacccgcat gcgggtgatt
    15541 atcgtccatg cgctgcgtaa cgcgatgctg ccggtagtga ccgttatcgg cctccaggtg
    15601 ggaacattgc tggcgggggc gattctgacc gaaaccatct tctcgtggcc cggtctgggg
    15661 cgctggttga ttgacgcact gcaacgccgc gactatccgg tagtgcaggg cggcgtattg
    15721 ctggtggcga cgatgattat cctcgtcaac ttgctggtcg atctgctgta cggcgtggtg
    15781 aacccgcgta ttcgtcataa gaagtaaggg gacatcatgt cacaggttac tgaaaataaa
    15841 gtgattagcg caccggtgcc gatgaccccg ttacaggagt tctggcacta ttttaaacgc
    15901 aacaaaggcg cggtcgtcgg gctggtttac gtcgccatcg tgctgttcat cgcgatcttt
    15961 gccaactgga ttgcgcctta taacccggcg gatcagttcc gcgatgcgct gctcgccccg
    16021 ccagcctggc aggaaggcgg cagtatggca cacttgctgg gcaccgatga cgtcggtcgt
    16081 gatgtgctgt cgcggctgat gtacggcgcg cgtttatcgc tgctggttgg ctgtctggtg
    16141 gtggtgttgt cgctgattat gggcgttatt ctcggcctga tcgccggtta cttcggcggc
    16201 ctggttgata acattatcat gcgcgtggtc gatatcatgc tggcgctgcc aagtctgctg
    16261 ttggcactgg tgctggtggc gattttcggc ccgtcgattg gtaacgccgc gctggcgctg
    16321 accttcgttg ccttgccgca ctatgtgcgc ttaacccgcg ccgccgtgct ggtggaggtg
    16381 aatcgcgatt acgtcaccgc ctctcgcgtg gcaggtgccg gggcgatgcg ccagatgttt
    16441 attaacatct tcccgaactg ccttgcgccg ctgattgttc aggcgtcgct cggtttctct
    16501 aacgccattc tcgatatggc tgctcttggt ttcctcggca tgggggcaca gccgccaacg
    16561 cctgagtggg gcaccatgct ctccgacgtg ttgcagttcg cgcaaagcgc ctggtgggtc
    16621 gtgaccttcc ctggtctggc gatcctgttg acggtgctgg catttaacct gatgggtgac
    16681 ggtctgcgtg acgcgctcga tcccaaactg aagcagtaag aggttcgaga tggcgttatt
    16741 aaatgtagat aaattatcgg tgcatttcgg cgacgaaagc gcaccgttcc gcgccgtaga
    16801 ccgcatcagc tacagcgtaa aacagggcga agtggtcggg attgtgggtg agtccggctc
    16861 cggtaagtcg gtcagttcac tggcgattat ggggctgatt gattatccgg gccgcgtaat
    16921 ggcagaaaaa ctggagttta acggccagga tttgcagcgt atctcagaaa aagagcgccg
    16981 caacctggtg ggtgccgaag tggcgatgat cttccaggac ccgatgacca gccttaaccc
    17041 atgctacacc gtgggtttcc agattatgga agcgattaaa gtgcatcagg gcggcaacaa
    17101 aagtacccgc cgtcagcggg cgattgacct gctgaatcag gtcggtattc ccgatccggc
    17161 ttcgcgtctg gatgtttacc cgcatcagct ttccggcggc atgagccagc gcgtgatgat
    17221 cgccatggcg attgcctgtc ggccaaaact gctgattgcc gatgaaccga ccaccgcgct
    17281 ggacgtgacc attcaggcgc aaatcatcga actactgctg gagctacagc agaaagagaa
    17341 catggcgctg gtgttaatta cccatgacct ggcgctggtg gcggaagctg cgcataaaat
    17401 cattgtgatg tatgcaggtc aggtagtaga aacgggcgat gcacacgcca tcttccatgc
    17461 gccgcgtcac ccgtatactc aggcattgct gcgtgcgctg ccagaatttg ctcaggacaa
    17521 agaacgtctg gcgtcgttgc caggtgttgt tcccggcaag tatgaccgcc cgaacggctg
    17581 cctgcttaac ccgcgctgcc cctatgccac tgacagatgt cgcgctgaag aaccggcgct
    17641 gaatatgctc gctgatgggc gtcagtccaa atgccattac ccacttgatg atgccgggag
    17701 gcccacacta tgagtacgca agaggccacc tcgcaacaac cgctgttgca ggctatcgac
    17761 ctgaaaaaac attatccggt gaagaaaggc atgttcgtgc cggaacgtct ggtgaaggcg
    17821 ctggacggcg tttcgtttaa ccttgaacgt ggcaaaacgc tggcagtagt gggcgaatct
    17881 ggctgcggta aatcgaccct cggtcggttg ctgacgatga tcgaaacgcc caccggcggc
    17941 gagctgtatt atcaggggca ggatctgctc aagcacgatc cgcaggcgca gaagctgcgt
    18001 cggcagaaaa tccagatcgt cttccagaat ccctacggtt cgctgaatcc gcgtaagaaa
    18061 gttgggcaaa ttctcgaaga gccgctgctt attaacacca gcttaagcaa agaacagcgt
    18121 cgggaaaaag ccctgtcgat gatggcaaaa gtcggcctga aaaccgagca ctatgaccgc
    18181 tatccgcaca tgttttccgg cggacaacgt cagcgtatcg ctattgcccg tggtctgatg
    18241 ctcgacccgg atgtggtgat tgccgatgag ccggtttctg cgctggacgt ctcagtgcgt
    18301 gcgcaggtgc tgaatctgat gatggatttg cagcaggagt tggggctttc ttatgtcttt
    18361 atctcccacg acctgtcggt tgtggagcac attgctgatg aagtgatggt gatgtaccta
    18421 ggccgctgcg tggagaaagg aacgaaggac caaatcttca ataacccgcg ccatccgtac
    18481 acccaggcgc tgctctccgc gacgccacgc ctgaacccgg acgatcgtcg tgaacgcatc
    18541 aaactcaccg gtgaactgcc aagcccgctg aatcctccgc cgggttgcgc cttcaacgcc
    18601 cgctgtcgtc ggcgcttcgg cccctgcacc cagttgcagc cgcagctaaa agactacgga
    18661 ggtcaactgg tagcttgttt tgctgttgat caggatgaaa atccgcagcg ttaactcaca
    18721 aaaccggggc catgcgctcc ggtttactca atcagcttca acagtggcga caagcatagc
    18781 aaaatgccgt aaagcagaat cagccaggct ttaaatccgc gaagtttttc cagttgtgcg
    18841 actttataga tcaggaaaaa cggaatgagg cacgacacaa tgccatataa cgggcttccc
    18901 aactgaaaga acaccagcac tgatacacga aacgaaaccc aaatcgtcaa cgtaatgacg
    18961 ataaaagcgc agatcgccag agtcagcatg cgtgagttaa ttttcttggt atcaataatt
    19021 cggcttaaca gattgagaat aatgccttta atagcctcgt ggaaaccgag ataaatgcca
    19081 aagaatgcgg tcagtacggc aaagatattc agcaccgtag aggtgatatg aatgatatgt
    19141 ccagggatca cctgcgcggc cagtgccagt gccgagatat tttgttcaaa ggcagaaacg
    19201 gcttcttcgt ggctaattga gaaggtaaac gaaaaggcaa aaaacaggat caccgcgatg
    19261 agcgtgatat aactaattcg gtgggtacgc agcgcgagcc gggttgccag tactttatct
    19321 gcttcccgtt tacgataggc aatattcatt gggttaagta cctgaataaa taccgcagaa
    19381 aagaagcaaa atggaatggt aagcaggaca tcgcggaaaa agacggaggc ttgcgggaag
    19441 gcggttatat tggcgaaatt ccagtgcggg atcatcgcaa aaccgaacac gacaataatc
    19501 cctactttga ccaccaccat tggcccagaa atcttaaaca gtaatcgttc accaccagac
    19561 gcaatcgcta tcagtacggc gaaaatagcg actttataaa gtagagattg tgaaagatcg
    19621 gcatcggtta aaccgaaggt tttcaggtac gaggcgctgt cgaaaaccac ggagagagag
    19681 tagataaaaa tcccgtggat aatcatcaaa aagtagataa ccccgaggaa aattccccag
    19741 ttcttcccca ggtaatgact gataatatcg gtgtagtcat tacaggaatc gctttcagaa
    19801 agggttttta aataaatgtc ctgcactacc caggtggcag gataagcaat gatcgctgcg
    19861 gtaataaata cccaaattcc cttcaagcca atttgtactg gcatcagcac ggttccggca
    19921 ccaatcgcca tgcctatgca taataaaacc cagccgaagt cgtagcgtgt aaacggcaat
    19981 ttctgattgt gtttcgatag tgtgttgtgc tgcataaagt cctgtccaga aagaagtcgg
    20041 cctcgcaaaa atgaataaac ccagtttctc ttgcgcaaca ggtggagaag tcgaatacgc
    20101 agtatgtaaa aaatgaggca acagattcac tggggaggtc gcgaggaggc gcaattctac
    20161 aaaattgcgc atttcactac cagtctggag tcagcccgga tatgaactgc aactattgca
    20221 aataaagaaa ttaagctgga aggcatcgcg caatgaggaa gattgccggg tgctgacagt
    20281 aaaaagcaat cgtaaaatca gcggcaagcc ggaagggttc tggtgaggcg caatgttgcg
    20341 ggggctttat ccctggtggc attggttgct ggagagagaa aacccccgca cgttgcaggt
    20401 atgtacctga caacataacg ggggctaatc ttgaacctag accactcaag aatagccgcg
    20461 aaacgttgtc attacaacac aggcggctat atgacgctcg cagagctggg catggccttc
    20521 tggcatgatt tagcggctcc ggtcattgct ggcattcttg ccagtatgat cgtgaactgg
    20581 ctgaacaagc ggaagtagcg tgtcatgtgg gcgtcaggct gccgtaatgg caatttgcgc
    20641 ccggaccagg ccgcaggggt gaaactctgc ggcctttttc gtaagaaaaa tgcaacggtt
    20701 gcaagcggat aatattctgc ggaatgcgca gcacagagtg aagatgagac ggattccgga
    20761 acagaaaaac agtcgtagaa tcagcgtcaa gccggaagga ttccggtgag gcgcaatgtt
    20821 gcgggggctt aatccctggt ggcattggtt gctggagaga gaaaaccccc gcacgttgca
    20881 ggtatgcacc tgacaacacc acgggggcca ttcttgcatc caaacaatac aagaatagcc
    20941 gcgaaacgtt gtcattacaa tacaggcggc tatatgacgt tcgcagaact gggcatggcc
    21001 ttctggcatg atttagcggc tccggtcatt gctggtattc ttgccagtat gatcgtgaac
    21061 tggctgaaca agcggaagta acgtgccatg cgggcgtcag gctgccgtaa tggcaatttg
    21121 cgcccggacc aggccgcagg ggtgaaactc tgcggccttt ttcgtaagaa aaatgcaacg
    21181 gttgcaagcg gataatattc tgcggaatgc gcagcacaga gtgaagacgt gacggattcc
    21241 ggaacagaaa aacagtcgta gaatcagcgt caagccggaa gggttccggt gaggcgcaat
    21301 gttgcggggg ctttatccct ggtggcattg gttgctggag agagaaaacc cccgcacgtt
    21361 gcaggtatgc acctgacaac atcacggggg ccattcttga acctgaacaa ctcaagaata
    21421 gccgcgaaac gttgtcatta caacacaggc ggctatatga cgctcgcaga actgggcatg
    21481 gccttctggc atgatttagc ggctccggtc attgctggca ttcttgccag tatgatcgtg
    21541 aactggctga acaagcggaa gtaacgtgtc atgcgggcgt caggctgccg taatggcaat
    21601 ttgcgcccgg accaggccgc aggggtgaaa ctctgcggcc tttttcgtaa gaaaaatgca
    21661 acgattgcac gcggataata ttctgcggaa tgcgcagcac agagtgaaga cgtgacggat
    21721 tccggaacag aaaaacagtc gtagaatcag cggcaagccg gaaaggttcc ggtgaggcgc
    21781 aatgttgcgg gggctttatc cctggtggca ttggttgctg gagagagaaa acccccgcac
    21841 gttgcaggta tgcacctgac aacatcacgg gggctaatct tgaacccaaa caactcaaga
    21901 atagccgcga aacgttggca ttacaacaca ggcggctata tgacgctcgc agaactgggc
    21961 atggccttct ggcatgattt agcggctccg gtcattgctg gcattcttgc cagtatgatc
    22021 gtgaactggc tgaataagcg gaagtaacgt gtcatgcggg cgtcaggctg ccgtaatggc
    22081 aatttgcgcc cggatcaggc cgcagaggtt aaactctgcg gcctttttcg tgcttactgc
    22141 gggtaaggca cccagtcgcc gccgttcagg cgaacgtacg gtttatcttg gtattgaata
    22201 actactgcat ttgagttctc ggagaccggt gctgtttgtg gcaacccact ggtgagtttt
    22261 ttccagtcaa cattgtcttc ggtgaaaatc ttgccatcaa gaacgcgaac caccagatcg
    22321 gagatagcca ggaagctgct cggttggtca atgacaatcg gtgccccctg atgtggtgcc
    22381 ttcatgccga agaatttcac cccaacgggg acgtctgtga tagacgggct agggatatca
    22441 cgtaggccag atacctgcat tctgtcgccc ttcagcgcgc cgccgtgttc cggcaccacg
    22501 accaccatca ctttacgacc cgatttctcc agttcagtaa agaaggcgtc cagttcatca
    22561 aagaatttct gcgcccgcgc tttgtaatcc gctgttttgc tgacccccgg ataatggttg
    22621 ccgtcatgca gtggaagcgt gttgtagaac gtggcactac ggctgttttt atctttttcg
    22681 gtaacgtcca gccagcggtt aagcacggcg gtatcgtcat aaaccggcga accatcaaag
    22741 cccagcaaaa taaccggcag atttgtttga tccatcaatt cagtctgcat gccgccattt
    22801 tcgcgaactt ctttcaaaaa accgccgaac tggccgttat gtcccatcat caggtgctgg
    22861 gtaaagccca gtttcgaaag gttatcaaac agatagcagt cgttatttgc cggttgatac
    22921 agattagtgt gcgaagtctg cccgcagctg gcgcgcagta aacggatcgc cgccgggcca
    22981 ctgtaggagg tggcggagtt aaagttcttg aactcaatat cgaaatgcga ccacagtgga
    23041 tgcgacatca acccggcggc ttctatatcc gaccaggaaa gcgaacagat gttaatcacc
    23101 agtagttcaa atggctgagc atcagcgggc agcgaagacg ggaaggtcga tttacgtttc
    23161 gcctccgcgt tatagaaatt attcagccag gcgttaaggt tcgccgttgt tggcggtgca
    23221 gtttgtgcgg gcatatcacc cactaccggt gcgccacccg tcgccgcaac ggttgccgct
    23281 gcgttaccac ccgtcgttgt tacagtggtc gtcggttgtc cggctggcca caaggagaaa
    23341 cttggtcccg ccagggtaag tacgttcagc catagcagta tggcaaccac aaaaacggta
    23401 atgcgaatcc attgtgacag gaataaccag gccactaata aaacaaaaat ggccccaatc
    23461 atctgccagt taataaagcg tgtgacaagg tcgattaaat aatcggtact gaaccccgcc
    23521 acctgcgaac cctggctcat tatgctttcc gggccaagca accaggtgtc atgccagaac
    23581 aaagcaaagc cgatcggcag ggcaatccag tggcgcaagc gatgcaggct gtaacgcgga
    23641 atgggcatca gcagaaacgc ggcaaacacc aaattgagga gcgggtggaa gttaagatat
    23701 cccgcccaca acaggccaaa cttaaccaga aaatagaagt tccagccgga aaggccgcgc
    23761 cagtattgcc agagggaaga aggcatggcg gtattttgcg taaattgagt cattttttgg
    23821 ttgccctggc tttttccgtg cggcgtaacg tcccggccgg tttaacataa cgaggtttag
    23881 caaagaacaa acgtaaggtg tcgcgaatgc gtcgcaaaga gtgccgcgcc agatagccca
    23941 gcgggaaaaa tatcagtgcg caaacgataa taatttcaat gatatcgctg atggtcatca
    24001 tgatgagcgc tccacagcat catctaacag tcgcatgggt tctggtattc gtcgccagtg
    24061 gcgaccatcg tgctcggcat tgatgaccgg tttagaactt tgcgttaaag gcagcggcat
    24121 tccccattgt tctggggcaa gcaggcgcat ctgcaccagc tcggcactga tttgatcatc
    24181 ttcaaaccag accatacggt ttgagaaaat gtcgccagta ggcaatggga aaatatgatt
    24241 caacgcggta tccagatcgt taatccgaca gaatgagaga aacagcacca gccgattacc
    24301 gccaatggtc atgatatcgc cggtacggtt agggcgacat agcgtcaggg cttgctcaac
    24361 gcggataccc ggtaccggac gtagggcaac cagaacgcct ttaccgtggg caggcaagag
    24421 agggttattc atcatgttgt tgacggcatt acagaacaca tcccacttct ggaaaccacg
    24481 cagtttgagc ggctgggtca ttgacagcaa ggtagtgata tcttccggta catagcgact
    24541 aaacttctgc ccttgcacgc tttcgatcat cgtcagacaa cgggagagtg gcgcattcca
    24601 cggaataacc atatttgcac cgcaggccaa taacaaacgt tcatcggtgg cgcgcaggct
    24661 agcggtattt tcccgcacga ggattttcat cgcactaccg cgctggcgac gcagggtatg
    24721 aatgctgcgg gccagtggct cgatttgcgc attttgctgt aaagaaaaga ccaccgtcgc
    24781 cgcctgagcg gtacgggctt cattgaacag gacttcgtta ttgttgaaca gttgccagtg
    24841 ttccgatagc ggcggcgcac cttccagtac agcaacatta ctcagaatgc gtttttcgtc
    24901 gctgcgtggt tggatctccg cctcttcgct ttgaactaat gtccagatac cattttgttg
    24961 ctgaacgcta agctgctgac gggcgctgac ccctttttcg ttgcaccaga aggcaatatc
    25021 cagcaaatgt tggtcaccct gaaaacgcaa actggcaaga ccaaaaagtg aacggtattc
    25081 ctca
//
LOCUS       DAETPZ010000055        24862 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.055, whole genome shotgun sequence.
ACCESSION   DAETPZ010000055 DAETPZ010000000
VERSION     DAETPZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.055"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            72..353
                     /locus_tag="K0W59_002542"
     CDS             72..353
                     /locus_tag="K0W59_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBI3023536.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            319..1395
                     /locus_tag="K0W59_002543"
     CDS             319..1395
                     /locus_tag="K0W59_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HBI3023537.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VAQAIEANHYIYKNYSPAALIEKLKGVDSFTMADWIERYKTILIRRKASRNTYKTRVN
                     QLVTIKENLGGVLLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKTYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHIRQIKTGMMIAIPLSLSLPVAGLRLGAVVEQCRMVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKLTGILFSENPPTFHEIRSLAGRLYKEICGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1788..2129)
                     /locus_tag="K0W59_002544"
     CDS             complement(1788..2129)
                     /locus_tag="K0W59_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HBI3023538.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2142..3014)
                     /gene="copD"
                     /locus_tag="K0W59_002545"
                     /pseudo
     CDS             complement(2142..3014)
                     /gene="copD"
                     /locus_tag="K0W59_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(3018..3392)
                     /gene="yobA"
                     /locus_tag="K0W59_002546"
     CDS             complement(3018..3392)
                     /gene="yobA"
                     /locus_tag="K0W59_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HBI3023539.1"
                     /translation="MASTARSLRYALTILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3531..3761
                     /gene="holE"
                     /locus_tag="K0W59_002547"
     CDS             3531..3761
                     /gene="holE"
                     /locus_tag="K0W59_002547"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HBI3023540.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3863..4519
                     /locus_tag="K0W59_002548"
     CDS             3863..4519
                     /locus_tag="K0W59_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBI3023541.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGNYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4543..5205
                     /gene="exoX"
                     /locus_tag="K0W59_002549"
     CDS             4543..5205
                     /gene="exoX"
                     /locus_tag="K0W59_002549"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HBI3023542.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5202..7262)
                     /gene="ptrB"
                     /locus_tag="K0W59_002550"
     CDS             complement(5202..7262)
                     /gene="ptrB"
                     /locus_tag="K0W59_002550"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HBI3023543.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQHWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPT
                     TPAD"
     gene            complement(7471..8130)
                     /locus_tag="K0W59_002551"
     CDS             complement(7471..8130)
                     /locus_tag="K0W59_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HBI3023544.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8457..8813)
                     /locus_tag="K0W59_002552"
     CDS             complement(8457..8813)
                     /locus_tag="K0W59_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HBI3023545.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDATGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8880..9170)
                     /gene="yebG"
                     /locus_tag="K0W59_002553"
                     /pseudo
     CDS             complement(8880..9170)
                     /gene="yebG"
                     /locus_tag="K0W59_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9304..10482
                     /gene="purT"
                     /locus_tag="K0W59_002554"
     CDS             9304..10482
                     /gene="purT"
                     /locus_tag="K0W59_002554"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HBI3023546.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10537..11178)
                     /gene="kdgA"
                     /locus_tag="K0W59_002555"
                     /gene_synonym="eda"
     CDS             complement(10537..11178)
                     /gene="kdgA"
                     /locus_tag="K0W59_002555"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBI3023547.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGVGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11215..13026)
                     /gene="edd"
                     /locus_tag="K0W59_002556"
     CDS             complement(11215..13026)
                     /gene="edd"
                     /locus_tag="K0W59_002556"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HBI3023548.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLFRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVRTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13261..14736)
                     /gene="zwf"
                     /locus_tag="K0W59_002557"
     CDS             complement(13261..14736)
                     /gene="zwf"
                     /locus_tag="K0W59_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBI3023549.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15075..15944
                     /gene="hexR"
                     /locus_tag="K0W59_002558"
                     /gene_synonym="yebK"
     CDS             15075..15944
                     /gene="hexR"
                     /locus_tag="K0W59_002558"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HBI3023550.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16072..17514
                     /gene="pyk"
                     /locus_tag="K0W59_002559"
     CDS             16072..17514
                     /gene="pyk"
                     /locus_tag="K0W59_002559"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBI3023551.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMHADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17645..18616)
                     /gene="lpxM"
                     /locus_tag="K0W59_002560"
                     /gene_synonym="msbB"
     CDS             complement(17645..18616)
                     /gene="lpxM"
                     /locus_tag="K0W59_002560"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBI3023552.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18736..20058)
                     /gene="mepM"
                     /locus_tag="K0W59_002561"
     CDS             complement(18736..20058)
                     /gene="mepM"
                     /locus_tag="K0W59_002561"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HBI3023553.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(20074..21006)
                     /gene="znuA"
                     /locus_tag="K0W59_002562"
     CDS             complement(20074..21006)
                     /gene="znuA"
                     /locus_tag="K0W59_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HBI3023554.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            21085..21840
                     /gene="znuC"
                     /locus_tag="K0W59_002563"
     CDS             21085..21840
                     /gene="znuC"
                     /locus_tag="K0W59_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HBI3023555.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21837..22622
                     /gene="znuB"
                     /locus_tag="K0W59_002564"
     CDS             21837..22622
                     /gene="znuB"
                     /locus_tag="K0W59_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HBI3023556.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22769..23779)
                     /gene="ruvB"
                     /locus_tag="K0W59_002565"
     CDS             complement(22769..23779)
                     /gene="ruvB"
                     /locus_tag="K0W59_002565"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBI3023557.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23788..24399)
                     /gene="ruvA"
                     /locus_tag="K0W59_002566"
     CDS             complement(23788..24399)
                     /gene="ruvA"
                     /locus_tag="K0W59_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBI3023558.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(24538..24603)
                     /gene="yobI"
                     /locus_tag="K0W59_002567"
     CDS             complement(24538..24603)
                     /gene="yobI"
                     /locus_tag="K0W59_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HBI3023559.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24674..>24862
                     /locus_tag="K0W59_002568"
     CDS             24674..>24862
                     /locus_tag="K0W59_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023560.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITT"
BASE COUNT         6165 a         6295 c         6108 g         6294 t
ORIGIN      
        1 ttattaaaaa tcaaccacac ccgccagtat tctgtatatt tactggcggt catatcgtga
       61 gaggtatggc aatgaatctt gtgacactca aaacgtgggg aaaactcaga tatccggata
      121 acccaccatc aatatcaacg ctgagacgat gggcaaggaa tggaaacatt tatcctgcac
      181 ctgaactaca cgggaggagt tacagggtgg ttccggaggc tttctatatc aacccaaata
      241 aggttgatac cgatataaca caccatcagc ctaatgggcg acaagggaga gacagtccgt
      301 taatggagaa gttaaaacat gcagcggaaa aaatacgatc ccaatttgcc tagaaactta
      361 acatatcgaa ggagagacaa agcatattac tggcgcaacc ctctgacgaa agaagaattt
      421 acactaggta aaatttcaag aagagatgca gtagcgcagg caattgaagc aaatcattat
      481 atatacaaaa actactctcc tgctgcccta attgaaaagc ttaaaggggt cgactcattt
      541 actatggcag actggattga acgttacaaa acgattctta taaggagaaa agcgtccaga
      601 aatacttata aaactcgggt aaatcaactg gtgacaataa aagaaaattt aggaggggtt
      661 ttactgacag aaataaccac tcgccatatc gccgagtttc ttgatttgtg gattgaagga
      721 gggaaaaaca cgatggcagg atcaatgcgt tctgtgttat ctgatatgtt ccgcgaagcc
      781 attgttgaag gacgtatatc tcaaaatcca gtaacgccaa caagagcacc gaaaatagta
      841 gttacaagag aacggctgaa actaaagaca tacaactgca tcagggaggc agcagatcaa
      901 cttccggcat ggttcccatt agctatggac ttagcccttg taacaggaca acgtcgcgaa
      961 gacataacga atatgcggtt cagtgatatt tatgatgatc gtctccacat caggcaaatt
     1021 aagacaggaa tgatgattgc tatccccctg tcactcagcc ttcctgtcgc tggtttacga
     1081 cttggtgcag tagttgaaca gtgccgcatg gtaagtaggg gggattatct aatcagtgcc
     1141 gggattagaa aaaacagccc tgacggcagc attcacccgg acggcctgac aaagaaattt
     1201 gtcgcagcca gaaaattaac aggtatcctg ttcagtgaaa acccaccaac ttttcacgaa
     1261 atcagaagcc tggctggacg attgtacaaa gaaatatgtg gagaagaatt tgctcagcgt
     1321 ctacttggcc acacatcgga gaagacaaca aaaatgtatc ttgatgagag agaaaaaacg
     1381 tacttactgc tctgatttta acgtaaatgg attgttaaat gtattttggt tgtgatataa
     1441 ccaaaaaaga ccggaataca gaaattcgag taaatttcgg ggaatttcgg ggagacgttt
     1501 gcaactaatt gattttaaat acaattaaaa aaagaccgaa tacgattcct gtattcggtc
     1561 cagggaaatg gctcttggga gagagccgtg cgctaaaagt tggcattaat gcaggcttag
     1621 ttgccttgcc ctttaagaat agatgacgac gccaggtttt ccagtttgcg tgcaaaatgg
     1681 tcaataaaaa gcgcggtggt catcagctta aatgttaaaa accgcccgtt ctggtgaaag
     1741 aactgaggcg ttttttttat tggaaatcaa aaggctattt taggtaatta acagagtttt
     1801 tcagctcgtt ctataaacgg tgccagactc attttttcgc cgggattgtt aggatcatca
     1861 atctgaatca ccgaaatggg ttgggcattg gtcttcccac tggcaacttc cttttgtgcg
     1921 atatcgttta aaggatactg cacgagggta cttgggttga tgacatacaa agcattaccc
     1981 ggtcggcaag tcagcattac ctcttcgcga ttaaacgccc atttgtcttt acccacttca
     2041 aaacgactca cggttatcac ctgtggagcc gccagcgccg ctgcagaact ggtgagtaac
     2101 agaaacgcca gaatactttt tttcatcatt ttcgtaatcc atcaaaaagg ttcgagagtc
     2161 gcaagcaggc tgatgatcac cagcactacc gcacctatcg cccattcgag tttagtcatc
     2221 cacacaaacc agggtgccgc acgatcttca tcctgcctca tgcgcggtac aagaacataa
     2281 cgattagcca gcgcaatgac caccataatc atcaccagga tggctttgag caataagagt
     2341 tggccctagt aagtcgtaag cgtgggggga aatccggtaa tcaacaatgc attaagcacg
     2401 ccgcttgcca gtacgccgat caccgcaaaa tgcccgcacc aggaaaaacg catcagcgcc
     2461 tgaatagcct ggtgtcgcca acggcctttg atgagttgca tacaccagag caccggcagt
     2521 aatcccccaa accacgccgc cgcacaaatc aggtgaatcg cgtgattagt ctgatggatt
     2581 ttcgctgtta ccccttcatt cagcgtcgca tgccccactc ccgccagcag aataaattgc
     2641 gcggtagtga gcataaacaa caaacgtggc atattccgcg gttgcataag ggcgacgatc
     2701 aacgttacga gggcgagaac aatttgccat aaccagacgc caccaaactg cgtttgcaaa
     2761 acagcctgcc aaatgtttgg cgaaaataca tctgtccatc ccgttcccat caacccgccc
     2821 tgaacggcga gcattgcagt ggcactgatc aaactccaga cggcagcgtg ctgttgtaaa
     2881 cgcagagaac gcttcgtcag caagcgacga atcattaagg gggccagcca ggcaccatac
     2941 attgcaaagc caaagaccag catcaacgag gtgaaatgga taaatcgcag cgcgatccag
     3001 gtaaacgcca gcatgattta tttcacgcta aaggtgtagt gtcctttcgt tttatgcccg
     3061 tccaccgaaa caacatgcca gtcaacggtg taggttccgg gtttcagcga atcggccagt
     3121 ggaacaatca gttgcttttg gtcctgctca ttccgcttcg ctggtaatgt tttaatattt
     3181 tcgttttttg gccccgtgat ttttgcacca ctgaatcctg tttcaacgcc ttccgagaag
     3241 tttaaagtga ttgcctgcgg ggcagctgtc acttgcgcgt ttgccgcagg atactgatgc
     3301 gttaaatgcg catgtgccca gacggaaggt gtcaccaacg aagtggtcag gattgtcagc
     3361 gcgtagcgaa gggagcgtgc agttgaagcc atattatcta ttcctttttg taataacttt
     3421 tttacagagc ataaccttgt ctaatgtctg agtcgaggat catcaattcc ggcttgccat
     3481 ccaggctcac tcttagtaac ttttgcccgc gaatgatgag gagattaaga atgctgaaga
     3541 atctggctaa actggatcaa acagaaatgg ataaagtgaa tgtcgatttg gcggcggccg
     3601 gggtggcttt taaagaacgc tacaatatgc cggtgatcgc tgaagcggtt gaacgtgaac
     3661 agccagaaca tttgcgcagc tggtttcgcg agcggcttat tgcccaccgt ttggcttcgg
     3721 tcaatctgtc acgtttacct tacgagccca aacttaaata aaacttatac agagttacac
     3781 tttcttacat aacgcctgct aaattatgag tattttctaa accgccccca taatttgcag
     3841 tcattttgaa aaggaagtca ttatgtcgtt ctggaaagtt gcagcagcgc aatatgagcc
     3901 tcgtaaaacc tcactcaccg aacaggtagc tcatcatctg gagtttgtga gggccgccgc
     3961 caggcaacaa tgtcagctgt tagtttttcc atcgctttct ttactggggt gtgattattc
     4021 gcgacgtgcc cttcctgccc cacccgatct ttcactgtta gacccgcttt gttatgccgc
     4081 aacgacctgg cgaatgacca tcattgccgg ccttcccgtt gaatataacg atcgctttat
     4141 tcgtggaatt gcggtgttcg caccgtggcg aaaaacgcct gggaactacc atcagagtca
     4201 tggtgcatgt ctgggccgtc gttccagaac catcacggtg gtcgatgaac aaccgcaagg
     4261 tatggatatg gacccgacct gttcactgtt tacgacaggg caatgtctgg gagaacccga
     4321 cctgctggcg tcggcccgtc gcttacaatt tttttcacat cagtactcga ttgccgtgct
     4381 gatggcaaat gcccgtggta acagtgcact atgggatgaa tatggtcgtc tcatcgttcg
     4441 cgccgatcgt ggttcattat tgttagtcgg tcagcgttca tcacagggtt ggcaaggcga
     4501 tatcattcca ttacgctagg ctttttcggc ctggagcatg ccatgttgcg cattatcgat
     4561 acagaaacct gcggtttgca gggagggatc gttgagattg cctctgttga tgtcattgac
     4621 ggaaaaatcg tcaaccccat gagcctcctg gtgcgccccg atcgtcctat tagtccacaa
     4681 gcgatggcga ttcatcgcat caccgaagcc atggtcgccg ataaaccgtg gattgaagat
     4741 gtgatcccac actattacgg tagtgaatgg tatgtcgcgc acaacgccag ctttgaccgc
     4801 cgcgtactgc ctgagatgcc cggtgagtgg atttgcacta tgaaactggc ccgtcgtttg
     4861 tggccagggc tcaagtacag caatatggcg ttatataaaa cacgcaagct caatgtacag
     4921 acaccgccgg gcctgcatca tcaccgcgcg ttgtatgact gttatatcac cgccgcgttg
     4981 cttatcgata ttatgaacac ctccggctgg acggcagaac agatggccga tatcaccgga
     5041 cgtccgtcgt tgatgacgac cttcaccttt ggcaaatacc gtggcaaagc ggtttccgac
     5101 gttgccgaac gcgatccggg ctatctgcgc tggttattta ataacctgga cagcatgagc
     5161 ccggagctgc gtttaacact gaaacattat ctggaaaata cttaatccgc aggcgtagtg
     5221 ggtaatgttc cctgcgccag cgcgaccaga aaagcatatt ccatcgctac gccttcgtac
     5281 gatttaaagc ggccagattt accgccatgg cctgagtcca tgtcggtaca gagcaataaa
     5341 agatggtcat cggttttcag ctcgcgcaat ttagcgaccc attttgccgg ttcccaatgt
     5401 tgcacctgag aatcgtgtaa accggtcgtt accagtaaat gcggataagc ctgtgcggtg
     5461 acgttgtcat acgggctgta gcttttcatg tactcgtaat attgcggatc ctgcggattt
     5521 ccccactctt caaactcacc agtggtaaga ggaattgatt catcaagcat cgttgttaca
     5581 acatcaacaa acggtacctg ggcgataacg ccgtggaata attccgggcg ttgattaatt
     5641 gcaacgccca tcaacatgcc ccccgcactt ccgcccatcg cataacagag cgaaggagag
     5701 ccatagccca gttttaacaa tgcatcgcag gcatcaagat aatcattaaa cgtatttttc
     5761 ttcttcagaa atttaccgtc ttcgtaccat tgttgcccca gctcaccacc gccgcgaaca
     5821 tggacaatgg cgtagacaaa gccacgatct aacaaactca agcggctaaa actgaaatcg
     5881 gcatcaatac ttgcgccgta agaaccatag ccatacacca gcaacgggtt gtgtcctttg
     5941 cgaaaatgtt tgcgatgata gaccagcgaa accggaactt cgacgccatc acgggcgact
     6001 atccacaggt gttcactgcg gtaattcgcc gcatcaaaac cgggcacttc tgtttgtttt
     6061 aatacacgac gctcaccggt atccatatcc agttcaaaca aagtgtctgg tgtggtcatg
     6121 gaagaataac cataacgcaa tcgcgcggtt tcaggttctg gattgtaggc aatccaggtc
     6181 acataggccg gatcatcaaa ggcaatgcca atgacttccc gggtcttgcg gttaatttgg
     6241 cgcaaactgg ttaacccgcg ctgacgctct tcaaccacca gccagtcggt aaacagcgta
     6301 aacccttcca gcatgatgtt ttcacgtggc ggaattaact cttcccactg ttgctcatca
     6361 cgcatacggg tacggtataa gccaaagttt ttgccgtggc gattggaacg cagataaaaa
     6421 cgatgctggt agtgatcaag gctgtattcg tgatctttgc ggcgcggcag aaaaacaaac
     6481 ggctcggcat cggccatttc cgcgtccagc aggcgaactt cactggtggt ggcgctggcc
     6541 agatgaatga cgacatagtg cttcgacgtc gttttatgca ggctgacata ataggtatcg
     6601 tctttttctt cgtagatcag tgtatcttgc gatgctggcg taccgatggc gtgacgccag
     6661 acctgataag gcagcagcgt caccggatgc ttgcgaacat agtagaaagt ccaggagtca
     6721 tttgcccaga caaagctggg ttcaacgtta tccagcagtt ccgggtacca gttacctgtt
     6781 tccagattac gaaaacgaat gccgtactgg cgtcgggaaa gaaaatcttc tgccagcgcc
     6841 ataatggtgt tatcgggcgt aatcgccatt ccgcccatcg aataaaactc actatgagct
     6901 gcgcgcttgt tggcatcgag caatgtttcc cactcatccc actcttcgct gaatgccgat
     6961 tgacgctggt agatagcata ttcacagcct ggttcataaa tatgccgata gcggtagcca
     7021 ttttttatgt agggcgcaga aacttctctt tgcggaatgc ggtcgatgat ttcctttaag
     7081 atgcgatcct gcaaggcttg ttgtgaggcc atcacccgat gaccgtaact attttcttgt
     7141 tgcaggtagt ccaggacttc tggctgagaa cgcgtatcgt cccgcagcca gtagtaatta
     7201 tcgatgcgcg tatcgccatg aagcgtcatg gcgtggggaa tgcgggcggc ttttggtagc
     7261 atgttattgt tctttctggt tgaaacatct tataagggtg gcaaaactca ccggggatgc
     7321 aagcgaaaca gggtcgtcat tgcttagatg atgacaggta atggcgcgga tatcgaatgt
     7381 tatgcagaca gagaaaatca gcctgttcaa atggctgtgc ggttctggat agcccgaaat
     7441 agtcaacttc aggctatcca gagagcggaa ttattccgcc aaagtgcgtt tttgctgttc
     7501 gagatcgcgt tcaatgcctt cacgaacatc ctgggggatt ttcagcgcgt cacccagtgc
     7561 attcaggtaa ctgcgttcca taaaatggtc aatatcaata gccgcgcagc tcaggaaata
     7621 gatttccagc gcctcttctt cattgcggac cccggtagcc aggcgttgtg gatccagcgg
     7681 ttgttcgatt gcctgctcaa tgagtacacg cccctgctct tccacgccgg cttcacgcaa
     7741 ttgctggtcg attgccgcac gttcattggc atcaatatga ccatcacttt tagcggcaaa
     7801 gaccagcgca aggatcaaac gttccgtacg ctcatccagc ggcgtacttt gcgcgccaaa
     7861 ctgcggttcg tcctgatgcg ccgcgcgaat tttatctttg tatttattcc acagcaccgt
     7921 acccgctacc gctccgccgc caaccagtaa cgcgttggtg ccatatttcg taagaagttt
     7981 acgtgctgat ttatttgcga ccagcagccc agccagtccg cctaatgcgc ctggcactaa
     8041 cagtttgccc aatccctgat ccgcagacga ggaggtagaa gaactgcttt gcccaagcag
     8101 ggattgcagt tgatttaacc agttagccat atttgctcct caataaccat ttttagtgcc
     8161 tgtagcatag cggatgagga tgtcagaaaa tgtatatcga gacaaaagat gcgcaaataa
     8221 gcggggcggt aggccgttat tcaaagaaat tcgcgccagg taaggtattt actgtatatc
     8281 aggtgataag ggtattttag gtgaaagtag tttgtcgttt tgagtggcat tgtttgatgc
     8341 tgattgcccg atgcgacgct gttgcgtctt ctcaggccta cgattgtggt gcgttctggg
     8401 acggacaagg cgctcgcgcc gcatccgcca acggtcaatc gaattatcag aagccattaa
     8461 cgccgctgat attccgccat tcccgctttg cagtccacgc tgacctggta atgaatatcg
     8521 gcacttttac cacgcacggt tagcggtact gaccatttat catctttacc ctgaatgtcc
     8581 tgcaaactga cccaggccac gggatcggcc tgaccgacaa ttttttgatc atctgcccag
     8641 cgcgccacgc gattttgttg ataatcacgt tttacgctcg cggcaattcc ggtagcatcc
     8701 agaccttcac actttgggaa agtgaccgac ttgctggttt cattattggc agcgaaaact
     8761 gatgcgcagg cagaaaccaa caaccgccct aaaaacgccc ctcttttttt catgtttttc
     8821 tccatagcac aatgattcag gagaaagcat ggtacaaatt gtcaggagcg caagttgctt
     8881 caggccgcgt gtgaggcatc ttcctcttcg gattcagcac cgacgaccag tggggacggt
     8941 aatttgccgg ttttcagaat ggtgctcagc acatcttttt gttctgccag ccatagcgaa
     9001 agggcttcac gttgctcgtc ttccatttgc actggagaat ttgtcagcta ggtgtcaagc
     9061 agatccgccg tatcaagcat tttgtcataa gcatcggctt cctttttgct ggtaaacgac
     9121 attttctctt cgccctcacg aatgactacg tatttaactt caaccgccat ttgcagcctc
     9181 tcataataac tgtgatttta tacagtatat ttcttttcgg ttgagaaatc aacatcagca
     9241 ataaagacac acgcaaacgt tttcgtttat actgcgcgcg gaattaatca ggggatattc
     9301 gttatgacgt tattaggcac tgcgctgcgt ccggcagcaa ctcgcgtgat gttattaggc
     9361 tccggtgaac tgggtaaaga agtggcaatc gagtgtcagc gtctcggcgt agaggtgatt
     9421 gccgtcgatc gctatgccga cgcaccagcc atgcatgtcg cgcatcgctc ccatgtcatt
     9481 aatatgcttg atggtgatgc attacgccgt gtggttgaac tggaaaaacc acattatatc
     9541 gtgccggaga tcgaagctat tgccaccgat atgctgatcc aacttgaaga ggaaggactg
     9601 aatgttgtcc cctgcgctcg cgcaacgaaa ttaacgatga atcgcgaggg tatccgtcgc
     9661 ctggcggcag aagagctgca gctgcccact tccacttatc gttttgccga tagcaaaagc
     9721 cttttccgcg aggcggttgc cgccattggc tatccctgca ttgtaaaacc ggtgatgagc
     9781 tcttccggca aggggcagac gtttattcgc tctgcagagc aacttgctca ggcatgggaa
     9841 tacgctcagc aaggcggtcg cgccggagcg ggccgcgtaa ttgttgaagg cgtcgttaag
     9901 tttgacttcg aaattaccct gctaaccgtc agcgcggtgg atggcgtcca tttctgtgca
     9961 ccagtaggtc atcgccagga agatggcgac taccgtgaat cctggcaacc acagcaaatg
    10021 agcccgcttg cccttgaacg tgcgcaggag attgcccgta aagtggtgct ggcactgggc
    10081 ggttatggtc tgtttggtgt cgagctgttt gtctgtggtg atgaggtgat tttcagtgag
    10141 gtctcccctc gtccacatga taccgggatg gtgacgttaa tttctcaaga tctctcagag
    10201 tttgccctgc atgtacgtgc cttcctcgga cttcctgttg gcgggatccg tcagtatggt
    10261 cctgcagctt ctgccgttat tctgccacaa ctgaccagtc agaatgtcac gtttgataat
    10321 gtgcagaatg ccgtaggcgc agatttgcag attcgtttat ttggtaagcc ggaaattgat
    10381 ggcagccgtc gtctgggggt ggtactggct acttcagaga gtgttgttga cgccattgaa
    10441 cgcgcgaagc acgccgccgg acaggtaaaa gtacagggtt aaacccggca aaaaacgcta
    10501 caaaaatgcc cgatcctaga tcgggcattt tgacttttac agcttagcgc cttctacagc
    10561 ttcacgcgcc agcttagtaa tgcggtcgta atcgcccgct tccagcgcat ctgccggaac
    10621 cagccaggaa ccaccgatgc acagcacgct tttcagtgcc aggtagtcac ggtagttagc
    10681 cggagaaata ccacccgtcg ggcagaaacg gacctgggag aacggacccg cgatcgcctg
    10741 cagggctttc acgccgccgt tagcttcagc cgggaagaat ttgaactctt tcaaaccgta
    10801 gtccataccc agcatcagtt cggaaacagt gctgatcccc ggaatcagag gaatagtccc
    10861 ttcggtagca gctttcagca gcggctcggt cagacccggg ctaattgcga actgtgcacc
    10921 cgcttcagtg acttctgcca gctgctgtgg attcagcacc gtaccgacac ccacaatcgc
    10981 ttcaggcact tctttggcga tagcacggat agcgtcaact gcacactcgg tacgcagagt
    11041 cacttccaga acgcgcaccc caccagcaac caacgctttt gccatcggca ccgcgtgttc
    11101 cagttttttt accacgataa ccggtacaac cgggccggtg gtcaggattg attctgcact
    11161 tgttttccag tttttcatca gagttttctc tcgcctgatt acaaatttgt cgtcttaaaa
    11221 agtgatacag gttgcgccct gttcggcacc ggacagtttt tcacgcaagg cgctgaataa
    11281 ttcacgtcct gttctcacgc gtgacgcgct caggtcagga atgtgcggtt cgcgagcagc
    11341 cagttccgct tcgtctacca gcagtgtcag ttcgcctgtc tgtccattca cacgaatgat
    11401 gtccccgtcg cgcacttttg ccagcagccc gccatcgtag gcttctggtg ttacgtggat
    11461 agctgacggc actttacctg aagcgccgga gagtcgtcca tcggtaacta acgcaatttt
    11521 gaaacaccgg tccaataata caccaagtgg cggcatgagt ttatgtaatt ctggcattcc
    11581 gttcgctttt ggcccctgat gacggacaac aacgacacaa tcgcggtcca gcaaacccgc
    11641 ttcaaaggcc ggcataacgt catgctggct ttcaaaaaca accgctggcg cttcaatcac
    11701 ctggttctca accggcacgg cagaggtttt cataaccgca cggcccaggt taccgcttaa
    11761 cacttttgtc ccaccatgat gagagaaagg ttgttcgaag gaagcgatca cattgctgtc
    11821 gagtgatttt tccgcccctt cccgccagtc cagttcacca ttattcagcc atggttcaag
    11881 ggtataacga aacagaccaa agcctgccac cgtattgaca tcttcatgca gcaggcctgc
    11941 tttgagcagt tcacgcacca gaaccggtac gccacctgcc gcctggaagt ggttaatatc
    12001 ggccggaccg ttcgggtaga gacgtgccat cagcggtaca acatcagaaa ggtcagagaa
    12061 gtcatcccag ttaatctgaa taccggccgc gcgcgccatc gccaccaggt gcatggtgtg
    12121 gttagtggaa ccaccggtcg ccaacagtgc aacgataccg ttcaccacca ctttctcatc
    12181 gatcatctta ccgatcggca tccattcatt accattaccg gtcatgcgtg taacctgacg
    12241 cgcagcggca gcggtcaaag catcgcgcag cggagaatcc ggatgaacaa aagaagagcc
    12301 tggcaattgc atccccataa attccaccac catctggttg gtgttggcag taccgtagaa
    12361 agtacatgtt cccggcgcat ggtaagacgc ggcttctgac tccagtaagg ccatgcggtc
    12421 cactttacct tcggcataaa gctggcgaat acgcactttt tctttatttg gcaaaccgct
    12481 tgccatcggt ccagacggca caaacaccgc aggcaaatga ccaaacgaca gggctgccat
    12541 cgtcagaccc gggacaatct tgtcgcacac accgaggaac agagcaccat caaacatgtt
    12601 atgggacagc cccaccgccg cagacatcgc tatcacttcg cggcttagca gcgacaattc
    12661 cattccatcc tgcccctggg tgacaccatc acacatcgcc ggaacaccgc ccgcaacctg
    12721 accaaccgca ttcgcttcat gcagggcttt acgaatgatt tctggatagt gttcataagg
    12781 ctggtgcgcg gagagcatgt cgttatagga ggtgatgatg gcgatattgt tacgcaacat
    12841 gcttttcaaa gaggctttgt cttctggctg gcaggcagcg aaaccgtgtg ccaggttacc
    12901 gcatgccaac tgcgaacgat gaacggtcga cgttttcgct tgttctatcc gggcgagata
    12961 agcagagcga gtctcgcgcg aacgttcaat gattcgattt gttacgcgta acaattgtgg
    13021 attcataaag gctcctgaaa ttgagttgtc agagcaggat gattcacaac gcgtttcatt
    13081 cagaggattt atgagtgaaa cgcctgtaac cggagctcat agggcaaaaa cgtttcagtc
    13141 agtgtaataa aaaaagcctc gtgggtgaat ccgcacgagg cctgaaagtg taaaaattgt
    13201 tctacaatct gtgcaagatc atgttaccgg taaaataacc ataaaggata agcgctgata
    13261 ttactcaaac tcattccagg aacgaccatc acgggtaatc atcgccaccg aggcaacggg
    13321 tccccaggtt ccggcctgat acggtttcgg cgcatcattg tccatcgccc acgcctcagt
    13381 aatggagtct acccatttcc aggcttcttc cacttcgtcg cgacgtacaa acagtgcctg
    13441 aataccacgc atggtttcca gcagcaaacg ttcataggca tccgccagat gcgtctgatt
    13501 aaaggtttct gaatagctca gatccagctt ggtgatttgc aggttatgtt tgtggtcaag
    13561 gccaggaact ttattcagta cctggatatc cacgccttca tcaggttgca gacggatagt
    13621 cagtttattc tgcggcagat cctgccacga ctctttaaac agattcagtt caggtgtttt
    13681 gaaatagacc acgacttcag aacatttggt cggcagacgt ttaccagtac gcaggtagaa
    13741 tggcacaccg gcccagcgcc agttatcaat gtcgacgcgg atcgccacga aggtttctgt
    13801 attgctgctt ttgttcgcgc cctcttcttc cagatatccc ggcacttttt tgccctgggc
    13861 gaagcccgca gtatattgcc cgcgtacggt tttttcgcgt acgttggagc ggtcgatgcg
    13921 gcgcagggac ttcagtactt tcactttttc atcgcggatg ctgtctgcgc tcaggtcaga
    13981 tggcggagac atcgcaatca tgcagagaat ttgtagcagg tggttctgaa tcatgtcacg
    14041 catctgaccg gctttatcaa aatagcccca gcgcccttcg atccccactt cttctgccac
    14101 ggtaatctca acatgatcaa tggtgcgatt gtcccagtta ttcacaaaca gggagttagc
    14161 aaaacgcagc gccaacaggt tcagcaccgt ttctttacca agatagtggt cgatacggta
    14221 aacctggcac tcctcgaagt attcgccaac ctgatcattg atttcctgcg aggtcgccag
    14281 cgacgtcccc agcggtttct ccatgactac gcgtgccggt ttagcattca gttttgcctc
    14341 gccaagccct ttgcaaattg cgccaaaagt gctgggcggc atggcaaagt agttaatagt
    14401 gatacgattt ttttgatcca gcatcgcgcc gagacggttg aatgcagcag tgtcattgac
    14461 atcgagatta caaaaatcca gacgtgcact cagggtgtcc cataaacctt catcaatggt
    14521 ttctttcatg aaagtttcga gcgcctcgcg gacaactttg gtatatgccg ctttatccca
    14581 gtcagcacgc cctacgccga taatccgggt gtccgggttg agctgaccgg ctttttccag
    14641 ttgatacagg gaaggcagca atttacgacg cgcaaggtcg cctttcgcgc cgaaaatgac
    14701 caggtcacag gcctgggctg tttgcgttac cgccatgtca ttctccttaa gttaactaac
    14761 ccggtactta agccagggta tacttgtaat tttcttacgg tgcactgtac tgcttttccg
    14821 agcttgcgaa aactgtaaac gcttatccac ccgtgcgatt acgggaaaag cgcgcaaagt
    14881 gcggcaaaac tgataaaaaa atcatcgttt tccattcggt aaaaatctga cactgctcat
    14941 gttatgaaaa aaaaataaca acttttttat ctgcttttgt cattaacacg gcacacaggc
    15001 gtaatatctg acaaaactgc atttcgattt ctttcagtgc ggaaatcgtc attacccgtg
    15061 agtctcttta catcatgaat atgctggaaa aaatccagtc tcagctggaa catttgagca
    15121 aatcagagcg caaagttgcc gaggtcattc tggcttcgcc cgataacgcg atccattcga
    15181 gtattgctgc tctggcgctg gaagccaatg ttagcgaacc gacggtgaat cgtttctgtc
    15241 gcagcatgga cacgcgcggt tttcctgatt ttaaacttca tctggcacaa agtctggcga
    15301 atggcactcc ctatgttaat cgcaacgtca atgaagatga cagcgttgaa tcatacacgg
    15361 ggaaaatatt tgagtccgca atggcaacgc tcgatcatgt ccgtcattca ctggataaat
    15421 ctgccatcaa ccgcgccgtc gacttgctca ctcaggcaaa aaaaatcgcc tttttcggat
    15481 taggctcttc tgccgccgtt gcccacgatg cgatgaataa gttctttcgt tttaatgttc
    15541 cggtggtgta ctccgatgat atcgtgctgc aacgcatgag ttgtatgaat tgtagcgacg
    15601 gagacgtggt ggtgctgatt tctcacacag gaagaacaaa aaatctggtc gagctggcgc
    15661 agctggcacg cgaaaacgac gccatggtga ttgccctcac ctctgcgggt accccgctcg
    15721 cccgggaagc aacgctggca attaccctcg acgtaccgga agatactgac atttatatgc
    15781 ccatggtttc tcgacttgca cagctgaccg tgatagatgt gctggcgaca ggatttactt
    15841 tgcgacgcgg tgcaaaattc agagataact tgaagcgggt caaggaagcg ctgaaggaat
    15901 cgcgttttga taagcagtta cttaatttaa gtgacgatcg ctaaaagcga ctgtcactgt
    15961 cctaatctta tacgacatcc gaatgagatt aatttatcgc catcgcggcg ttatttcatt
    16021 cggatttcat gttcaagcaa cacctggttg tttcagtcaa cggagtatta catgtccaaa
    16081 aggcttcgca gaacaaaaat cgttaccacg ttaggcccag caacagatcg cgataataat
    16141 cttgaaaaag ttatcgcggc gggtgccaac gttgtacgta tgaacttttc tcacggctcg
    16201 cctgaagatc acaaaatgca cgcggataaa gttcgtgaga ttgccgcaaa actggggcgt
    16261 catgtggcta ttctgggtga cctccagggg cccaaaatcc gtgtatccac ctttaaagaa
    16321 ggcaaagttt tcctcaatat tggggataaa ttcctgctcg acgccaacct gggtaaaggt
    16381 gaaggcgaca aagaaaaagt cggtatcgac tacaaaggcc tgcctgctga cgtcgtgcct
    16441 ggtgacatcc tgctgctgga cgatggtcgc gtccagttaa aagtactgga agttcagggc
    16501 atgaaagtgt tcaccgaagt caccgtcggt ggtcccctct ccaacaataa aggtatcaac
    16561 aaacttggcg gcggtttgtc ggctgaagcg ctgaccgaaa aagacaaagc agacattaag
    16621 actgcggcgt tgattggcgt agattacctg gctgtctcct tcccacgctg tggcgaagat
    16681 ctgaactatg cccgtcgcct ggcacgcgat gcaggatgtg atgcgaaaat tgttgccaag
    16741 gttgaacgtg cggaagccgt ttgcagccag gatgcaatgg atgacatcat cctcgcctct
    16801 gacgtggtaa tggttgcacg tggcgacctc ggtgtggaaa ttggcgaccc ggaactggtc
    16861 ggcattcaga aagcgttgat ccgtcgtgcg cgtcagctaa accgagcggt aatcacggcg
    16921 acccagatga tggagtcaat gattactaac ccgatgccga cgcgtgcaga agtcatggac
    16981 gtagcaaacg ccgttctgga tggtactgac gctgtgatgc tgtctgcaga aactgccgct
    17041 gggcagtatc cgtcagaaac tgttgcagcc atggcgcgcg tttgcctggg tgcggaaaaa
    17101 atcccgagca tcaacgtttc taaacaccgt ctggacgttc agttcgacaa tgtggaagaa
    17161 gctattgcca tgtcggcaat gtacgcagct aaccacctga agggcgttac ggcgatcatt
    17221 accatgaccg aatcgggtcg taccgcgctg atgacctccc gtatcagctc tggtctacca
    17281 attttcgcca tgtcgcgcca tgaacgtacg ctgaacctga ctgctctcta tcgtggcgtt
    17341 acgccggtgc actttgatag cgctaatgac ggcgtagcag ctgccagcga agcggttaat
    17401 ctgctgcgcg ataaaggtta cttgatgtct ggtgacctgg tgattgtcac ccagggcgac
    17461 gtgatgagta ccgtgggttc tactaatacc acgcgtattt taacggtaga gtaagtacgt
    17521 tgccggatgc ggcgaaaacg ccacatccgg cctatagttc aatgatagtt caacagattt
    17581 cgaatattct gaagcaaact tgaacttatc atcaggcgaa ggcctctcct cgcgagaggc
    17641 ttttttattt gatgggataa agatctttgc gcttatacgg ctggatttcg cccggtttgc
    17701 gagttttcag caattttagt atccaggtgt attgttctgg tcgcggacca acaaaaatct
    17761 cgacttcttc attcatccgc cgctcaatcg tatgatcatc cgcctctaac agatcatcca
    17821 tcggtgggcg cacctgaatc gtcagacgat gcgtcttgcc atcataaatc ggaaacagcg
    17881 gtacaacgcg cgcacggcat actttcatca aacgaccaat cgcgggcaac gtcgctttat
    17941 aggtggcaaa gaaatccaca aattcgctgt gctctgggcc atgatcctga tcgggtaaat
    18001 aatatcccca gtacccctga cgtaccgact ggatgaatgg tttaataccg tcatttctcg
    18061 catgcagacg accgccaaag cgacgacgca ccgtgttcca gacataatca aaaaccgggt
    18121 tgccctgatt atggaacatc gctgccattt tctgcccttg cgaggccatc agcatggcag
    18181 gaatatcgac ggcccaaccg tgcggcacca gaaagataac tttctcgtta ttacgccgca
    18241 tctcttcgat gatctccagc ccttgccagc caacgcgcgg ctgaattttc tccggcccgc
    18301 gtattgccaa ctcagccatc attgccatcg cttgcggcgc ggtggcaaac atctcatcaa
    18361 caatcgcttc gcgttcagct tcactacgtt ctggaaagca gagcgacaga ttgattaacg
    18421 cacgacggcg tgagcttttt cccagtcgtc cggcaaaacg tcccagccgt gccagaatgg
    18481 gatcacggaa ctttggcggc gttaaagcga tacccgccat cgctgctaca cccagccatg
    18541 ctccccagta gcgcgggtgg cgaaaggatt tatcaaactc aggaatgtat tcgctattat
    18601 tttttttcgt ttccatgctt ttccagtttc ggataaggca aaaatcaatc tggtgatagt
    18661 gtagcggcgc aacttgcccc gcaccaaata aaaaaagccg gtactgactg cgtaccggct
    18721 gcgaatggat gttaattaat caaaccgtag ctgcggcaca atctctttgg cctgcgccag
    18781 gaattcgcga cgatcggagc cggtcagccc ttcggtacgc ggcagttttg ccgtcagcgg
    18841 gtttacggcc tgctggttta tccatacttc atagtgcaga tgcggcccag ttgaacgtcc
    18901 ggtattaccg gaaagcgcga tacggtcgcc acgtttcacc ttctgtcccg gtttcaccag
    18961 aatcttgcgc aagtgcatat aacgcgtggt gtagctgcga ccatgacgaa tagccacata
    19021 ataacctgct gcgccactgc gtttggcaac caccacttca ccgtcaccca ctgaaagcac
    19081 tggcgtacct tgtggcatgg cgaaatcaac acctctgtgt ggtgcaacgc gacctgtcac
    19141 cggattagtt cgacgcgggt taaagttaga cgagatacgg aactgtttcg ccgtcgggaa
    19201 tcgcaagaat cctttcgcca gaccagtacc gttacgatcg tagaatttgc catcctcagc
    19261 gcggattgcg taataatctt taccttctga acgcaaacgt acgcccagca gctggctttg
    19321 ctcacgttta ccatcaagca tttctcgaga cattaatacc gcaaattcat cgcctttttt
    19381 cagtttgcgg aaatccattt gccactgcat ggctttaatc actgcgctca cttcggcgct
    19441 ggttaaaccg gcgtttctgg cgctggcaac aaagctccct ccgacggtac ctttcagcag
    19501 attgttaacc cactctcctt gctgcatttc gctggtcatt ttaaaaccgt tagcggcagt
    19561 acggtcatag gttcgggttt cacgacgaga cacttcccag gtgaggcgct gcagttcgcc
    19621 gtccgcggtt aatgtccagg agagttgttg accgattttc aggttacgca attctttgtc
    19681 ggcagcagcg agttgggtga tatcacccat atcaatacca tactgattga gaatgctgct
    19741 tagcgtatcg ccagtggaaa caacatattc atgcacgccc gcttcaccgg cgattttgtc
    19801 atccagttcg tcctggggaa tggcttcatc ttcttgtgca gcttgatcaa tcggctcact
    19861 ggcttcaggt aagagcgaac gaatttcgtt ctgttccagc tcaatggttt tgacaattgg
    19921 cgtggcatca cggtgataaa catagggccg ccagacagcg acggccagag taagaacggt
    19981 gagcgacccc aacataacgc ggtgtggtcg cggtaaatta ttaaacgcca gggcgacaga
    20041 gcgggctatc tgttgcacgt aatcacttcc tcattaatct cctttcaggc agctcgcata
    20101 ctggttggct aattgactca ggaattctga atagcttgtt ttacccagtt tgatattcgt
    20161 ccccagggga tccaacgttc ccatacgaac ggatgtccct cgtgcgacgc tctcaacgac
    20221 cgctggcctg aactgtggct cagcaaaaac gcaggttgct ttttgctcaa ccaactgtgt
    20281 tcttatttca tgtaaacgct gcgcgccagg ttgaatctca gggttaacgg taaaatgacc
    20341 aagcggtgtc agtccgaact gtttttcgaa atagccgtaa gcatcgtgaa aaacgaaata
    20401 acctttcccc ttgagcggcg cgagctcgtt accaacctgc gtttcggttg aggccaattg
    20461 tgcctcaaaa tccttcaggt tggcgtcaag tttggctcga ctttgcggca taagttccac
    20521 taattttcca tggattgcaa ccgctgtagc ccgcgctatc tctggggaaa gccaaagatg
    20581 catgttgaaa tcgccgtgat ggtgatcttc gtcacttttt tccgcgtggt cgtgatcatc
    20641 atcatcgccg tgaatacttt tcatcagtag cggtttcaca tcttcaagct gcgcaatcgt
    20701 tacctgcttc gcttctggta atttgcttac cggtttttgc ataaacgctt ccatctccgg
    20761 gccaacccaa acgactaagt ccgcgttctg taagcgtttt acatccgatg gacgcagtga
    20821 ataatcatgt tctgaagcgc cgtcaggcag taaaacctcc gtttctgtta caccatcagc
    20881 aatggcagaa gcgatgaacc caacgggttt aagcgaagcg acaacggcgg catctgcggc
    20941 ctgtgttgca cctccccaga gagcggcgga taatgctgcg aaaagaagcg tttttttatg
    21001 taacataatg cgaccaatca tcgtaatgaa tatgagaagt gtgatattat aacatttcat
    21061 gactactgca agactaaaat taacatgaca agtctggttt ccctggaaaa tgtctcggtt
    21121 tcttttggcc aacgccgcgt cctctctgat gtgtcgctgg aacttaaacc tggaaaaatt
    21181 ttgactttac ttgggccaaa tggcgcaggt aagtcgacac tggtacgggt agtgctcggg
    21241 ctggtaacac ccgatgaagg ggttatcaaa cgcaacggaa aactgcgcat cggctatgta
    21301 ccgcagaagc tgtatctcga caccacgttg ccactgaccg taaaccgttt tttacgctta
    21361 cgccctggta cacataaaga agatattttg cctgcactga aacgtgtcca ggccgggcac
    21421 ctgattaacg caccgatgca aaagctctct ggtggcgaaa cgcagcgtgt actattagcg
    21481 cgagcattgt taaattgccc gcaattatta gtgctggatg aacccactca aggcgtggat
    21541 gtaaatggcc aagtggcgtt atatgacctt attgaccagt tgcgtcgtga actggattgt
    21601 ggcgttttaa tggtttctca cgatctgcat ctggtgatgg caaaaaccga tgaagtgctg
    21661 tgcctgaatc accacatttg ttgttccggc acaccggaag ttgtttccct gcatccggag
    21721 tttatttcaa tgtttggtcc tcgtggtgct gaacaactgg gtatctatcg ccatcatcat
    21781 aatcatcgtc acgatttaca gggacgaatt gttttgcgtc ggggaaatga tcgctcatga
    21841 ttgaattatt atttcccggt tggttagccg ggatcatgct cgcctgtgcc gcgggtccgc
    21901 tgggttcgtt tgtagtctgg cgtcgtatgt cttatttcgg tgatacgctg gctcatgcct
    21961 cattacttgg tgtcgcgttt ggtttgttgc tggacgtgaa tccattctat gcggtgattg
    22021 ccgttacgct gctgctggcg ggcggtctgg tatggctgga gaagcgtcca cagctggcga
    22081 tcgacacgtt attagggatt atggcgcaca gtgccctgtc gctgggccta gtggtcgtta
    22141 gtctgatgtc taacattcgt gttgatttga tggcttacct gttcggtgat ttactggcag
    22201 tgacgccaga agatctcatc tctattgcga ttggcgtggt catcgtggtg gctattttgt
    22261 tctggcaatg gcgcaatttg ctgtcgatga cgattagccc ggatctggcg tttgttgatg
    22321 gtgtgaaatt acagcaagtg aaattgttgt tgatgctggt gacggcattg acgattggtg
    22381 tagcgatgaa atttgtcggt gcgttgatta ttacttcgtt gctgattatt cctgcagcta
    22441 ctgcgcgtcg ctttgcccgt acgccggaac agatggctgg tgtcgctgtt ttggtgggga
    22501 tggtggcagt aactggcggt ttaacctttt ccgcgtttta cgatacgccg gcgggtccgt
    22561 cggtggtgct atgtgcggca ctgttattta ttatcagtat gatgaaaaag caggccagct
    22621 aatctatcgc tgaacacatt tgtcggatgc ggcgcgagcg ccttatccga cctacggttc
    22681 gctatctctg gctggcctga taagacgcga acagtgtcgc atcagacaca ctgccagtgt
    22741 cggatgcggc gcgagcgacc aatccgactt acggcatttc tggcggcgtt atgccaaagt
    22801 gattccacgc ccgcgtcgtc gccatacgcc cgcgcggtgt acgctgcaaa aagccttgct
    22861 gaatcaaata aggttccagt acatcttcaa tggtttcacg ttcttcgcca atggctgccg
    22921 ccaggttatc cagacctacc ggcccaccaa agaacttatc gattaccgcc agcaacaatt
    22981 tgcggtccat ataatcgaaa ccttcagcat cgacattcaa catatccagc gcctgagcag
    23041 cgatatctgc cgagatggtg ccatcgtgct tcacttcggc gaaatcacgc actcgacgca
    23101 gcagacggtt ggcaatacgt ggcgtaccgc gagcgcgacg agctacttcc tgcgcaccgt
    23161 catcactcat ctcaagcccc ataaagcgtg cactgcgact gacgatatat tgcagatccg
    23221 gcacctgata aaactccaga cgttgcacaa taccaaaacg gtcgcgcaac ggtgatgtca
    23281 gcgaacctgc gcgcgtggtt gcgccaatca gggtaaacgg cggcaaatca attttaatgg
    23341 agcgtgccgc cggaccttca ccaatcatga tatccagttg gtagtcttcc attgccgggt
    23401 acagcacttc ttcaacaact ggcgataaac ggtggatctc atcaataaac agcacgtcat
    23461 gcggttcaag gttagtgagc atcgcagcca aatcgcccgc cttttccagc accggaccag
    23521 aagtcgtgcg taaattaacg cccatttcat tggcgacaat gttggcaagc gtagttttac
    23581 ccaaccccgg aggaccaaaa atcaacaaat gatcgagggc atcgccgcgc agtttcgctg
    23641 ctttgatgaa aatctccatc tgtgaacgaa cctgcggctg accaacatac tcttccagta
    23701 atttagggcg aatggcgcga tctgctacat cttccggcaa agtggtaccg gcagaaatca
    23761 gacggtctgc ttcaatcatc ctttacctca taacgcggcg cgtagggctt cgcgaattaa
    23821 tgtttcactg ctggcgtcag ggcgagcgat tttgctcacc atgcggcttg cttcttgtgg
    23881 tttatagcct agcgccacca gcgcggcaac tgcttcttgt tcagcatcgt cggtcgccgg
    23941 gctggcagga gacgtgagta ccaggtcggc ggctggcgta aagagatcgc catgcaaacc
    24001 tttaaatcgg tctttcattt caacaatcaa gcgttcggcg gtttttttgc caatacccgg
    24061 cagtttcacc agtgccccca cttcttcacg ctcaacggca ttaacgaact gctgcgctga
    24121 cattccggag aggatcgcca gggccaactt cgggccgacg ccgttggttt tgatcaactc
    24181 tttgaacaac gtgcgttctt gtttattgtt aaaaccgtac agcagttgcg cgtcttcacg
    24241 caccacaaag tgggtgaaaa cgatcgcttc ctgacccgct tcagggagtt cataaaaaca
    24301 ggtcatcggc atatgcactt catagcctac gccgcccact tcaattaaca ccagcggggg
    24361 ttgtttttca atgatgatgc ctctgagtct gcctatcaca tgacgctcct gcgtaatgaa
    24421 tcaaagataa tgctgtatga taaaaaaatg ctggatagat atccagcgta ggatgaagaa
    24481 aacttgcgag gtgtcccgat gatctgaaaa atggcacagt ataatttatt caacaggtta
    24541 tattggaagc aaatatttga atattacata ttcagtgaag aaatgcgtaa taaaaatata
    24601 cattgcgact cctgaaaaaa taattttttt atgctattac gtatattcgt atctatttca
    24661 atggaatgac aacgtgaata ttaattaccc tgctgaatat gaaattggag atatcgtctt
    24721 tacatgtata ggtgctgcct tatttggtca aatatcagct gcatcaaatt gctggagtaa
    24781 tcacgtcggg atcattatcg gtcataacgg tgaagacttc ctggttgcag aaagccgggt
    24841 tcccctttca accatcacca cg
//
LOCUS       DAETPZ010000056        24568 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.056, whole genome shotgun sequence.
ACCESSION   DAETPZ010000056 DAETPZ010000000
VERSION     DAETPZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24568
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.056"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..87
                     /locus_tag="K0W59_002569"
     CDS             <1..87
                     /locus_tag="K0W59_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3023561.1"
                     /translation="GRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(102..593)
                     /locus_tag="K0W59_002570"
                     /pseudo
     CDS             complement(102..593)
                     /locus_tag="K0W59_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="required for cellulose biosynthesis; incomplete;
                     partial in the middle of a contig; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
     gene            866..1054
                     /locus_tag="K0W59_002571"
     CDS             866..1054
                     /locus_tag="K0W59_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HBI3023562.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            1066..1776
                     /gene="bcsQ"
                     /locus_tag="K0W59_002572"
     CDS             1066..1776
                     /gene="bcsQ"
                     /locus_tag="K0W59_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HBI3023563.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAEEILTLA
                     NWCLLNYSGLKTPVGSAS"
     gene            1773..4386
                     /gene="bcsA"
                     /locus_tag="K0W59_002573"
                     /pseudo
     CDS             1773..4386
                     /gene="bcsA"
                     /locus_tag="K0W59_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            4397..6736
                     /gene="bcsB"
                     /locus_tag="K0W59_002574"
     CDS             4397..6736
                     /gene="bcsB"
                     /locus_tag="K0W59_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBI3023564.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCEKPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNLPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIREYGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6743..7849
                     /gene="bcsZ"
                     /locus_tag="K0W59_002575"
     CDS             6743..7849
                     /gene="bcsZ"
                     /locus_tag="K0W59_002575"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HBI3023565.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7908..8084
                     /locus_tag="K0W59_002576"
                     /pseudo
     CDS             7908..8084
                     /locus_tag="K0W59_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            8412..10160
                     /gene="bcsC"
                     /locus_tag="K0W59_002577"
                     /pseudo
     CDS             8412..10160
                     /gene="bcsC"
                     /locus_tag="K0W59_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10242..12230
                     /gene="hmsP"
                     /locus_tag="K0W59_002578"
     CDS             10242..12230
                     /gene="hmsP"
                     /locus_tag="K0W59_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HBI3023566.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            12414..13700
                     /gene="dctA"
                     /locus_tag="K0W59_002579"
     CDS             12414..13700
                     /gene="dctA"
                     /locus_tag="K0W59_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HBI3023567.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            13921..15417
                     /locus_tag="K0W59_002580"
     CDS             13921..15417
                     /locus_tag="K0W59_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBI3023568.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(15513..16442)
                     /gene="kdgK"
                     /locus_tag="K0W59_002581"
     CDS             complement(15513..16442)
                     /gene="kdgK"
                     /locus_tag="K0W59_002581"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HBI3023569.1"
                     /translation="MSKKIAVIGECMIELSEKDADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGAGSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSVENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16674..17441
                     /gene="pdeH"
                     /locus_tag="K0W59_002582"
     CDS             16674..17441
                     /gene="pdeH"
                     /locus_tag="K0W59_002582"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HBI3023570.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            17511..19571
                     /locus_tag="K0W59_002583"
     CDS             17511..19571
                     /locus_tag="K0W59_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBI3023571.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLHIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(19753..21075)
                     /locus_tag="K0W59_002584"
     CDS             complement(19753..21075)
                     /locus_tag="K0W59_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBI3023572.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21486..22499)
                     /gene="yhjD"
                     /locus_tag="K0W59_002585"
     CDS             complement(21486..22499)
                     /gene="yhjD"
                     /locus_tag="K0W59_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HBI3023573.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22548..23447)
                     /locus_tag="K0W59_002586"
     CDS             complement(22548..23447)
                     /locus_tag="K0W59_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3023574.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            23967..>24568
                     /locus_tag="K0W59_002587"
     CDS             23967..>24568
                     /locus_tag="K0W59_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBI3023575.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5822 a         6188 c         6756 g         5802 t
ORIGIN      
        1 ggacggaagt cactgtcgtt ctcaaaatcg gtggagctgc atgacaaggt catcgggcat
       61 tatctgaaca taaaacacta tcagtaagtt ggagtcatta cctcctcaag caacaatgaa
      121 aattgtttat cgttattatt tccgggatta attaccaaaa gcgaacaatg gtttaacctg
      181 ctccatttat tcattttatc caaccatgag cgaagccgct cggcaggaat gttttgccat
      241 gcgttatttg cgcaaacaag aataaaaagg taattatggg gatcaataga acactgcaaa
      301 tcacgggcca aatagtatag acctttttca tgatttagca ttgaaaataa tttcattttt
      361 tccggccctt gagaatcatc taattgaaag attttcgccg gatcgctgtc catgctaatg
      421 accgcgacgt gtgcggtttc agcctgggat gcaattgttt gattcgccag actgatagca
      481 tcttcatggc gatcgacgtt aaaccaccag acgccgcctg ctggcatatg tcgcagctca
      541 tcccataatg atgagatacc gatagagaat acagggtcca caatgtccct catgtcacca
      601 ttttgtctga ttatcagttt actagcgaaa gctaagaaat aaacctacca ttgaaattaa
      661 aacttatcag atttagcatg tgaatcaatt tagaaggtca caagacgtgt gattttagtc
      721 ttttcgtttt taactttttc tgttgcgtaa cggaaagtca aaaagtgagc aaattcccgt
      781 ctcgccgtaa acaataaacc ggcgaaatgc tcacggttag aattgtatca tgaatggtac
      841 ggttatttca tagggatcaa gcaaaatgaa taacaatgaa ccagatactc tgcctgatcc
      901 cgcgataggc tatatcttcc agaatgatat tgtggcgtta aagcaggcat tttcactgcc
      961 tgatattgat tatgccgata tttcccaacg cgaacagttg gccgcggcat taaaacgctg
     1021 gccgttgctg gcagagtttg cgcaacaaaa gtaggggatt ggtgaatggc cgtactggga
     1081 ttgcaggggg tgcggggagg cgtggggaca acaaccatca ccgccgcatt agcctggtca
     1141 ttacaaatgt tgggagaaaa tgtcctagtg gtcgatgcct gcccggacaa cttgttgcgc
     1201 ctgtcattta atgttgattt tacccaccgt cagggctggg ccagagcgat gctggatggc
     1261 caggactggc gtgacgctgg gttgcgctac acctcgcagc tcgatttgct gccttttggt
     1321 cagttatcca ttgaagaaca agaaaatcca cagcactggc aaacccggct gagcgatatt
     1381 tgctccggct tacagcaact aaaagccagc gggcgttacc agtggatttt aatcgactta
     1441 ccgcgtgatg cctcgcagat aacccaccag ctgctgagtt tgtgcgagca ctcgctggca
     1501 atcgtcaatg tggatgccaa ctgccatatc cgactgcatc agcaagcgct gccggatggc
     1561 gcacatattt tgattaatga cttccgtatt ggcagtcagg ttcaggacga tatttaccag
     1621 ctttggttgc aaagccagcg ccgattactg ccgatgctca ttcatcgtga tgaagcgatg
     1681 gctgaatgcc tggcggctga agagatactg acgctggcga actggtgcct gttgaactac
     1741 tccgggctga aaacgccagt cgggagtgca tcatgagtat cctgacccgg tggttgctta
     1801 tcccgccggt caacgcgcgg cttatcgggc gttatcgcga ttatcgtcgt cacggtgcgt
     1861 cggctttcag cgcgacgctc ggctgtttct ggatgatcct ggcctggatt tttattccgc
     1921 tggagcaccc gcgctggcag cgtattcgcg cagaacataa aaacctgtat acgccaacgc
     1981 ctcgcgtccg cgtccgctgg acccggtccg ttatctcatt caaacatgct ggttattgat
     2041 cggtgcatcg cgcaaagaaa cgccgaaacc gcgcagcggg cattttcagg tctgcaaaat
     2101 attcgtggac gttaccatca atggatgaac gagctgcctg agcgcgttag ccataaaaca
     2161 cagcatctgg atgaaaaaaa agagctcggt catttgagtg ccggggcgcg gcggttgatc
     2221 ctcggtatca tcgtcacctt ctcgctgatt ctggcgttaa tctgcgttac tcagccgttt
     2281 aacccgctgg cgcagtttat cttcctgatg ctgctgtggg gggtagcgct gatcgtacgg
     2341 cggatgccgg ggcgcttctc ggcgctaatg ttgattgtgc tgtcgctgac cgtttcttgc
     2401 cgttatatct ggtggcgtta cacctctacg ctgaactggg acgatccggt cagcctggtg
     2461 tgcgggctta ttctgctctt cgctgaaacg tacgcgtgga ttgtgctggt gctcggctac
     2521 ttccaggtag tatggccgct gaatcgtcag ccggtgccat tgccgaagga tatgtcgctg
     2581 tggccgtcag tggatatctt tgtcccgact tacaacgaag atctcaatgt ggtgaaaaat
     2641 accatttacg cctcgctggg tatcgactgg ccgaaagaca aactgaacat ctggatcctc
     2701 gatgacggcg gcagggaaga gtttcgccag tttgcgcaaa acgtgggggt gaaatatctc
     2761 gcccgcacca ctcatgaaca tgcgaaagcg ggcaacatca acaatgcgct gaaatatgcc
     2821 aaaggcgagt tcgtgtcgat tttcgactgc gaccacgtac caacgcgatc gttcctgcaa
     2881 atgaccatgg gctggttcct gaaagagaaa cagctggcga tgatgcagac tccacaccac
     2941 ttcttctcgc cggacccgtt tgaacgcaac ctgggacgtt ttcgtaaaac gccgaacgaa
     3001 ggcacgctgt tctatggtct ggtgcaggat ggcaacgata tgtgggacgc cactttcttc
     3061 tgcggttcct gtgcggtgat tcgtcgtaag ccgctggatg aaattggcgg cattgctgtc
     3121 gaaaccgtga ctgaagatgc gcatacttct ctgcgtttgc accgtcgtgg ctatacctcc
     3181 gcgtatatgc gtattccgca ggcggcgggg ctggcgaccg aaagtctgtc ggcgcatatc
     3241 ggtcagcgta ttcgctgggc gcgcgggatg gtacaaatct tccgtctcga taacccgctc
     3301 accggtaaag gactgaagtt tgctcagcgg ctgtgctacg tcaacgccat gttccacttc
     3361 ttgtcgggca ttccacggct gatcttcctg actgcgccgc tggcgttcct gctgcttcat
     3421 gcctacatca tctatgcgcc agcgttgatg atcgccctat tcgtgctgcc gcatatgatc
     3481 catgccagcc tgaccaactc gaagatccag ggcaaatatc gccactcttt ctggagtgaa
     3541 atctacgaaa cggtgctggc gtggtatatc gcaccaccga cgctggtggc gctgattaac
     3601 ccgcacaaag gcaaatttaa cgtcaccgcc aaaggtggac tggtggaaga agagtacgtc
     3661 gactgggtga tctcgcggcc ctacatcttc cttgttctac tcaacctggt gggcgttgcg
     3721 gtaggcatct ggcgctactt ctatggcccg ccaaccgaga tgctcaccgt ggtcgtcagt
     3781 atggtgtggg tgttctacaa cctgattgtt cttagcggcg cagttgcggt atcggtagaa
     3841 agcaaacagg tacgccgatc gcaccgcgtg gagatgacga tgcccgcggc aattgcccgc
     3901 gaagatggtc acctcttctc gtgtaccgtt caggatttct ccgacggtgg tttggggatc
     3961 aagatcaacg gtcaggcgca gattctggaa gggcagaaag tgaatctgtt gcttaaacgc
     4021 ggtcagcagg aatacgtctt cccgacccag gtggcgcgcg tgatgggtaa tgaagttggg
     4081 ctgaaattaa tgccgctcac cacccagcaa catatcgatt ttgtgcagtg tacgtttgcc
     4141 cgtgcggata catgggcgct ctggcaggac agctacccgg aagataagcc gctggaaagt
     4201 ctgctggata ttctgaagct cggcttccgt ggctaccgcc atctggcgga gtttgcgcct
     4261 tcttcggtga agggcatatt ccgtgtgctg acttctctgg tttcctgggt tgtatcgttt
     4321 attccgcgcc gcccggagcg gagcgaaacg gcacaaccat cggatcaggc tttggctcaa
     4381 caatgatgat aacgcgatga aaagaaaact attctggatt tgtgcagtgg ctatggggat
     4441 gagtgcgttc ccctctttca tgacgcaggc gacgccagca acgcaaccac tgatcaatgc
     4501 tgagccagct gtagccgccc agacggaaca aaatccgcag gtggggcaag tgatgtcagg
     4561 cgagcaggga gctgatgccc ccatcgtggc gcagaacggt ccttcgcgtg atgtgaagct
     4621 gacctttgcg caaatcgcgc cgccgccggg cagcatggtg ctacgtggta ttaacccgaa
     4681 cggcagcatt gagtttggta tgcgcagcga tgaagtggtg acgaaggcga tgctcaacct
     4741 cgaatacacg ccatcgccat cgttactgcc tgtccagtcg cagttaaagg tttatctcaa
     4801 tgatgagctg atgggcgtgc tgccagtgac caaagaacag ttgggtaaaa aaacgctggc
     4861 gcaaatgccc attaatccac tgtttattac cgacttcaac cgtgtgcggc tggagtttgt
     4921 cggccattat caggacgtgt gcgaaaaacc ggccagcacc acgctttggc tggatgttgg
     4981 gcggagcagt ggactggatc tgacctatca gaccctgaat gtgaagaatg acctgtcaca
     5041 cttcccggtg ccattctttg acccgcgcga taaccgtact aacaccttgc cgatggtctt
     5101 tgcgggtgcg ccggatgttg ggctgcaaca agcatctgcc attgtcgcct cgtggtttgg
     5161 ttcgcgttct ggctggcgtg ggcagaacct cccggtgctc tataaccaat tgccggatcg
     5221 caatgccatt gtctttgcaa ccaacgacaa acggccggac ttcctgcgcg atcatccggc
     5281 ggtaaaagcc ccggtgattg agatgattaa ccatccgcag aatccttacg tcaaactgct
     5341 ggtggtgttt ggtcgtgacg acaaagacct gttgcaggca gcgaaaggta tcgctcaggg
     5401 taacattctg ttccgtggtg aaagcgtggt agtgaatgaa gtgaaaccgc tgctaccgcg
     5461 taagccgtac gatgcgccga actgggtacg taccgatcgt ccggtcacat ttggcgaact
     5521 gaaaacctat gaagaacagt tacaatccag cggccttgag ccagcagcga ttaacgtttc
     5581 gctaaacctg ccgccggatc tctacctgat gcgcagtacc ggcattgata tggatattaa
     5641 ttaccgctac accatgccgc cggtgaaaga cagttcgcgg atggatatca gcctgaataa
     5701 ccagttcctg caatccttca acctgagcag caaacaggag gcgaaccgtc tgctgctgcg
     5761 tattccggta ttacaaggtt tgctggatgg caaaacagat gtctctattc cggcgctgaa
     5821 actgggcgcg accaaccagc tgcgcttcga ctttgagtat atgaacccga tgccaggcgg
     5881 ttcggtagat aactgtatta ccttccagcc ggtgcagaat catgtggtga ttggtgacga
     5941 ttccaccatc gacttctcga agtattacca cttcatcccg atgccggatc tacgcgcctt
     6001 tgctaacgcg ggcttcccat tcagccggat ggcggatctg tcgcaaacca tcaccgtgat
     6061 gccgaaagcg cctaacgaag cacagatgga aacgttgctg aatactgttg gttttatcgg
     6121 cgcacagacg ggcttcccgg cgattaatct gacggtgacc gatgatggca gcaccattca
     6181 gggcaaagat gccgacatca tgatcatcgg tggtatcccg gacaaactga aagacgataa
     6241 gcagatcgac ctattggtgc aggcgaccga aagctgggtg aaaacaccga tgcgccagac
     6301 cccgttcccc ggcattgtgc cggacgagag cgatcgcgcg gcagaaaccc agtcaacgct
     6361 gacctcttcc ggtgcgatgg cggcggtgat tggcttccag tcgccgtata acgaccagcg
     6421 cagcgtgatt gcgctgttgg cagatagccc acgcggttat gaaatgctta acgatgcggt
     6481 gaacgatagc ggcaaacgcg ccaccatgtt cggttcggtc gcggtgatcc gcgagtacgg
     6541 tatcaacagc ctacgtgttg gcgacgttta ttacgtaggt catctgccgt ggttcgagcg
     6601 cttgtggtat gcgctggcaa accatccgat tctgctggcg gtgctggcgg ctatcagtgt
     6661 gatattgctg gcatgggtac tgtggcgtct gctgcgaatt attagtcgtc gtcgtcttaa
     6721 cccggataac gagtaattga agatgaatgt gttgcgtagt ggaatcgtga cgatgctgtt
     6781 gttggctgcc tttagtgttc aggcagcctg tacctggcct gcctgggagc agtttaaaaa
     6841 ggattacatc agtcaggaag ggcgcgtcat cgaccccagc gacgcgcgca aaatcaccac
     6901 ctccgaaggg caaagttacg gtatgttctt tgccctggcg gctaacgacc gtgcagcttt
     6961 cgataatatt ctcgactgga cgcagaacaa tctcgctcag ggttctttaa aagaacgttt
     7021 gcccgcctgg ctgtggggca agaaagagaa cagtaagtgg gaagtgctgg acagcaattc
     7081 ggcctccgat ggtgatgtct ggatggcctg gtcgttgctg gaggcggggc gtttgtggaa
     7141 agagcagcgt tataccgaca tcggcagcgc gttgctaaaa cgtatcgcgc gggaggaagt
     7201 ggtgacggtg cctgggctgg gttccatgtt gttaccgggc aaagtgggtt ttgctgagga
     7261 taacagctgg cgttttaacc ccagctacat gccgccgacg ctggcgcagt atttcacccg
     7321 ctttggcgcg ccgtggacca cgctgcgcga aaccaatcaa catttattgc tggaaaccgc
     7381 cccgaaaggc ttttcgccag actgggtgcg ctatgagaaa gacaaaggct ggcagctaaa
     7441 agccgaaaaa acattgatca gcagctacga cgctatccgc gtttacatgt gggtaggcat
     7501 gatgcctgac agcgatccgc aaaaagcgcg gatgctcaac cggtttaaac cgatggcgac
     7561 attcactgag aaaaacggtt atccgccgga aaaagtggat gtggctacgg ggaaagcgca
     7621 gggtaaagga ccggtcggtt tttctgccgc catgctgcct tttttacaaa accgcgatgc
     7681 gcaggcagtt cagcgccagc gcgtggccga taactttccc ggcagcgata cctattacaa
     7741 ctatgtgctg accctgtttg gacaaggctg ggatcaacac cgtttccgct tctcgacaaa
     7801 aggtgagtta ttacctgact ggggccagga atgcgcaaat tcacactaaa catattcacg
     7861 ctttccctcg gtctggccgt catgccgatg gtcgaggcag caccaaccat cagcagaccc
     7921 tgcggatgga cagcggcaac actaatgccg tgcgcgggct ggcgaatatt taccgccagc
     7981 agtcgccaga aaaagctgaa gcgtttatcg cctcgctctc tgccagccag cggcgcagca
     8041 ttgatgatat cgaacgcagc ctgcaaaacg accgtctggc acagcaggca gaggtactgg
     8101 aaaaccaggg caaatgggcg caggcggcag cacttcagcg gcaacgactg gcgctggacc
     8161 ccggcagcgt atggattact taccgacttt cgcaggatct ctggcaggcc ggacaacgca
     8221 gccaggccga tacgttaatg cgcaatctgg cgcagcagaa gccgaacaac ccggagcagg
     8281 tttacgctta cgggctgtac ctctcaggtc acaaccagga cagagcggcg ctggcgcata
     8341 tcaacagcct gccgcgcgcg cagtagaaca gcaatattca ggagctggtt aatcgactgc
     8401 aaagcgatca ggtgctggaa accgctaatc gcctgcgaga aagcggcaaa gaggctgaag
     8461 cggaagcgat gctgcgtcag caaccacctt ccacgcgtat tgacctcacg ctggctgact
     8521 gggcgtaaca acgacgtgat tacaccgccg cccgcactgc atatcagaat gtcctgacgc
     8581 gggagccaac taacgccgat gccattcttg gtctgacgga agtggatatt gctgccggtg
     8641 acaaagcggc ggcacgtagc cagctggcga aactgcctgc caccgataac acctcgctga
     8701 acactcagcg gcgcgtggcg ctggcacagg cgcagcttgg agataccgca gcggcgcagc
     8761 agacgtttaa taagttgatc ccgcaggcaa aatctcagcc accgtcgatg gaaagcgcga
     8821 tggtgctgcg cgacggtgcg aagtttgaag cgcaggcggg cgatccaaag caggcgctgg
     8881 aaacctacaa agacgccatg gtcgcatccg gtgtgactac gacgcgtccg caggataacg
     8941 acacctttac ccgactgacc cgtaacgacg agaaagatga ctggctgaaa cgcggcgtgc
     9001 gcagcgatgc ggcggacctc tatcgccagc aggatcttaa cgtcaccctt gagcacgatt
     9061 actggggttc gagcggcacc ggtggttact ccgatctgaa agcgcacact accatgttgc
     9121 aggtggatgc gccgtattct gacgggcgga tgttctttcg cagtgatttc gtcaatatga
     9181 acgtcggcag tttctccact aatgccgatg gcaaatggga tgacaactgg ggcacctgta
     9241 cattacagga ctgtagcggc aaccgcagcc agtcggattc cggtgccagc gtggcggtcg
     9301 gctggcgaaa tgacgtttgg agctgggata tcggtaccac gccgatgggc ttcaacgtgg
     9361 tggatgtggt cggcggcatc agttacagcg atgatatcgg gccgctgggt tacaccgtta
     9421 acgcccaccg tcggcccatc tccagttctt tgctggcctt tggtgggcaa aaagactccc
     9481 cgagcaatac cgggaaaaaa tggggtggcg tacgtgccga cggtgtgggg ctaagtctga
     9541 gctacgataa aggtgaagta aacggcgtct gggcatcgct tagtggcgac cagttaaccg
     9601 gtaaaaatgt cgaagataac tggcgcgtgc gctggatgac gggctattac tataaggtca
     9661 ttaaccagaa caatcgccgc gtcacaatcg gcctgaacaa catgatctgg cattacgaca
     9721 aagatctgag tggctactca ctcggtcagg gcggttacta cagtccgcag gaatacctgt
     9781 cgtttgccat accggtgatg tggcgggagc gcacggaaaa ctggtcgtgg gagctggggg
     9841 cgtctggctc gtggtcgcat tcacgcacca aaaccatgcc gcgttatccg ctgatgaatc
     9901 tgatcccgac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
     9961 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    10021 ttggcacggc aattgatatc cagcaggcga aagattacgc acccagccat ttcctgctct
    10081 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    10141 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    10201 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    10261 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    10321 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    10381 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    10441 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    10501 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgtccggtgc
    10561 cggtaatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtctactcgc
    10621 tcgaacgtcc ggcaaatccg cagccaatag cctatctggt gctacaggcg gattccttcc
    10681 gtatgtataa gttcgtgatg agcactctct caacgttagt gaccatttac ttacttttgt
    10741 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    10801 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    10861 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    10921 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    10981 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    11041 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    11101 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    11161 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    11221 gtgtacagga accgtggcac gcaatcactt tgggtcagca agtactcact atcatgagcg
    11281 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    11341 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    11401 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagcagccc
    11461 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcaa
    11521 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    11581 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    11641 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    11701 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    11761 aactgatgca cccgaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    11821 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    11881 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    11941 gtatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    12001 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    12061 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    12121 cacaacgaga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    12181 gcccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    12241 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    12301 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaaggc
    12361 cgcaggtacc cccataacct tacaagacct gtggttttac taaaggacac cctatgaaaa
    12421 cctctctgtt taaaagcctt tactttcagg tcctgacagc gatagccatt ggtattctcc
    12481 ttggccattt ctatcctgaa ataggcgagc aaatgaaacc gcttggcgac ggcttcgtta
    12541 agctcattaa gatgatcatc gctcctgtca tcttttgtac cgtcgtaacg ggcattgcgg
    12601 gcatggaaag catgaaggcg gtcggtcgta ccggcgcagt cgcactgctt tactttgaaa
    12661 ttgtcagtac catcgcgctg attattggtc ttatcatcgt taacgtcgtg cagcctggtg
    12721 ccggaatgaa cgtcgatccg gcaacgcttg atgcgaaagc ggtagcggtt tacgccgatc
    12781 aggcgaaaga ccagggcatt gtcgccttca ttatggatgt catcccggcg agcgtcattg
    12841 gcgcatttgc cagcggtaac attctgcagg tgctgctgtt tgccgtactg tttggttttg
    12901 cgctccaccg tctgggcagc aaaggccaac tgatttttaa cgtgatcgaa agtttctcgc
    12961 aggtcatctt cggcatcatc aatatgatca tgcgtctggc acctattggt gcgttcgggg
    13021 caatggcgtt taccatcggt aaatacggcg tcggcacact ggtgcaactg gggcagctga
    13081 ttatctgttt ctacattacc tgtatcctgt ttgtggtgct ggtattgggt tcaatcgcta
    13141 aagcgactgg tttcagtatc ttcaaattta tccgctacat ccgtgaagaa ctgctgattg
    13201 tactggggac ttcatcttcc gagtcggcgc tgccgcgtat gctcgacaag atggagaaac
    13261 tcggctgccg taaatcggtg gtggggctgg tcatcccgac aggctactcg tttaaccttg
    13321 atggcacatc gatatacctg acaatggcgg cggtgtttat cgcccaggcc actaacagtc
    13381 agatggatat cgtccaccaa atcacgctgt taatcgtgtt gctgctttct tctaaagggg
    13441 cggcaggggt aacgggtagt ggctttatcg tgctggcggc gacgctttct gcggtgggcc
    13501 atttgccggt agcgggtctg gcgctgatcc tcggtatcga ccgctttatg tcagaagctc
    13561 gtgcgctaac taacctggtc ggtaacggcg tagcgaccat tgtcgttgct aagtgggtga
    13621 aagaactgga ccacaaaaaa ctggacgatg tgctgaataa tcgtgcgccg gatggcaaaa
    13681 cgcacgaatt atcctcttaa tcccaccact tatgcccgca ctcccttccc cgagtgcggg
    13741 tgtttgcgca taattgaccc ctgtgcctga acttaccacc tgggtgagtt caggtttatt
    13801 tcacttcttc cgtgacattt tcatgttctt gcggtctaac acgaagtgtt tttaacgtca
    13861 tttagactga ccgacaacgg acagtttttt tattaccagg atagttgatc aggggttcac
    13921 atgcagggca taaaaattcg acttttagcg ggcggtttgc tgatgatggc cactgctggc
    13981 tatgtgcagg cagatgcgct ccagcctgat ccagcatggc aacaggggac gctttccaac
    14041 ggtttacagt ggcaagtgct gaccaccccc cagcgtccca gcgatcgtgt tgaaattcgc
    14101 ctgctggtta ataccggttc gctcgccgaa agtacacaac agagcggtta cagtcacgcc
    14161 atccctcgta ttgcgctaac gcaaagcggt ggccttgacg cagcacaggc gcgttcattg
    14221 tggcagcagg ggatcgaccc taaacgcccg atgccgccgg taattgtctc ttatgacacc
    14281 acgctgttta atctgagttt gcccaataac cgtaacgatt tgctgaaaga agcgctctct
    14341 tatctggcaa atgccactgg caaattgacc atcacaccag aaaccatcaa ccacgcgctg
    14401 caaagtcagg acatggtggc aacctggcct gccgatacta aagagggctg gtggcgctat
    14461 cgtctgaaag ggtcaacctt gttaggtcac gatcctgccg atccgctgaa acaacccgtt
    14521 gaagcggaaa aaattaaaga tttctatcag aaatggtaca ccccggatgc aatgacgcta
    14581 ctggtggtgg gaaacgtgga tgcgcgctcg gttgtcgacc aaatcaacaa aacgtttggc
    14641 gaactgaaag gcaaacgtga aacgccagct ccggtgccga cgctttctcc gctgcgtgcg
    14701 gaagcggtga gtattatgac tgacgcggtg cgtcaggacc ggttatctat catgtgggat
    14761 acgccgtggc agccgattcg tgaatcagcc gcactgctgc gctactggcg tgcggacctg
    14821 gcccgcgagg cgctgttctg gcatgttcag caagcgttaa gcgccagtga cagcaaagac
    14881 atcggtcttg gatttgactg ccgtgtgctg tatctgcgtg cgcagtgtgc catcaacatc
    14941 gaatcaccaa acgacaagct gaacagcaac cttaatctgg tggcgcgtga actggcgaag
    15001 gttcgcgata aaggtctgcc ggaagaagag ttcaatgcgt tagtggcgca aaagaaactg
    15061 gagctgcaga aactgtttgc cgcctatgca cgggctgata ccgatattct gatgggccag
    15121 cggatgcgtt cgttgcaaaa tcaggtcgtc gatatcgcgc cggagcagta tcagaaactg
    15181 cggcaggatt tccttaacag cctgactgtg gagatgttaa atcaggatct gcgtcagcag
    15241 ttgtcgaatg atatggcgtt aatactgctg cagccgaaag gcgagccgga atttaacatg
    15301 aaagcgttgc aggcggcctg ggatcaaatc atggccccat ctaccgccgc tgcgaccacc
    15361 tctgtcgcca cggatgacgt acatcctgaa gtgacggata ttccacctgc acagtaatgc
    15421 ctgatggtga tgagttagca caaccatcag gtatttatgg atgagctgga tagaggggta
    15481 acactttatc cagccttttg catatgctgc gtttacgctg gcatcgcctc acgcgggata
    15541 atcgcgccgc gatactgaat aacggtactt gcggtcaggt gcccgcgttt cgccgcgttt
    15601 tccacactgc cgcccgtcaa acgtaccgcc agataaccgg cgctgaaaga gtcgccagct
    15661 gcggtggtat cgatcacttt ttctttcggc agtttcaccg ccggaacatc aactaacgct
    15721 tcaccagcaa tggacaccag gcaagaaccc gccccgcgtt tcaccaccac ttctttcacg
    15781 cccgcgttat gggtgcgcgc aatgacgtct tccaccggct gttgacccca cagcgcgtct
    15841 tcgtcgtcca gcgtcaggaa ggcgatatcc gtgcattcca gcatttgctg gtacacctgc
    15901 tgtgtctctt ctttgctggc ccacaggcgc ggacgatagt tattgtcgaa aatcactttt
    15961 ccgcctttgg cgcggcattc gcgcagcagg gaaagcagct tttcgcggct ggtcgggctt
    16021 aagatcgcca ggctaatccc gctcaggtag agataatcga aattcgccag ctcttcgcaa
    16081 atcgccgcag actgctcgct cgccagccag aatttggcgg cggcttcgtt acgccagtag
    16141 tagaacgtac gttcgccggt gctgtcggtt tcaatgtagt aaaggcccgg aagacggttt
    16201 tccatccgtt gggtcaggga agtatcaacg ttctcgccgt gccaggcgtc cagcatctgc
    16261 tggctaaaac tgtccgttcc cagcgccgtt acgtaatgaa cggttaatgc cgcaggatcg
    16321 acctgacggg cgatatagac ggaagtgttc agggtatcgc cgccgaaacc gcgcttaacg
    16381 tccgcgtctt tctcggaaag ctcaatcatg cattcgccaa tcacggcaat ctttttagac
    16441 atagtcgtga acctgatctg taaaaaatta gcgttagttt gcgctgtggt ggtttgctgg
    16501 tcaaccatat taaaacagtg ttccattatt tttttgagcc agaacgtatt cctgaaagat
    16561 tccgttgtgg agtgaggaaa tttaccccgg ccaggcggta aagttctgcc cttacgcgcc
    16621 gataatcttt gtcgagtccg ggcagcatca cttttaaaca caggacatct ttgatgataa
    16681 ggcaggttat ccagcgaata agcaaccctg aagcaagcat cgagagcttg caggaacggc
    16741 gtttttggtt gcagtgtgag cgtgcttaca cctggcagcc gatctatcaa acatgcgggc
    16801 ggttaatggc cgtggagcta ttaacggtgg tcacgcatcc cttgaaccct tcgcaacgcc
    16861 tgccgccgga tcgctatttt actgaaatca ccgtcagcca tcggatggag gttgtgaaag
    16921 agcagattga tttgctggcg caaaaagccg acttctttgt agagcacggc ctgctggcat
    16981 cggtcaatat tgatggccct acgctcatcg ccctgcgtca gcaaccaaaa atcctgcgcc
    17041 agattgagcg tcttccctgg ctgcgtttcg aactggtgga gcatatccgt ctgccgaaag
    17101 attcaacctt tgcctcgatg tgtgaatttg gcccgctgtg gctggatgat tttggtaccg
    17161 ggatggcaaa tttctctgca ctaagtgaag tgcgttatga ctacatcaaa atcgcgcgag
    17221 aactgtttgt gatgctgcgt cagtcgccgg aaggccgcac actcttttct cagcttttac
    17281 atctaatgaa tcgctattgt cgcggggtga ttgtcgaggg cgtagaaaca ccggaagagt
    17341 ggcgtgatgt tcagaactcg cccgcattcg ccgcacaagg ctggtttctt tcacgcccgg
    17401 caccgatgga aacgctgaat acggcggttc tggcgctata agctgcctca ttttccgccg
    17461 ggctggacta tctttaggac tggcacagga agaatatgag gaagcgaacg atgagcaagg
    17521 caggcaaaat aaccgctgcg atttcagggg ctttcttgtt gttgattgtc gtggcgatca
    17581 ttttgattgc aacttttgac tggaatcgac tcaaaccgac catcaaccag aaagtctctg
    17641 cggagttgaa tcgtccgttc gctatccgtg gcgatctggg cgtggtgtgg gagcggcaaa
    17701 aacaagaaac tggctggcgc agctgggtgc cgtggcctca tgtacatgca gaagacatca
    17761 ttcttggtaa tccaccggat attcccgaag tcacgatggt gcatttgccg cgagtagagg
    17821 caacgctggc accgctggcg ctgctgacca aaacggtctg gctgccgtgg atcaagctcg
    17881 aaaagcccga cgcgcgcctg attcgcctct ctgaaaagaa caataactgg acgtttaatc
    17941 ttgccaacga tgacaacaaa gacgcgaatg caaagccgtc ggcatggtcg tttcggctgg
    18001 ataatattct tttcgatcaa gggcggatcg ccattgatga caaagtaagc aaagcggatc
    18061 tggaaatttt tgttgatccg ttaggcaagc cgctgccatt cagcgaagtt actggatcga
    18121 aaggtaaagc ggataaagaa aaggtgggcg attacatttt tggcctgaag gcgcagggac
    18181 gttataacgg tgaaccgctc acgggtacgg gaaaaatagg cggtatgctg gcgctgcgtg
    18241 gcgaagggac gccgtttccg gtacaggctg atttccgttc aggtaatacc cgtgttgctt
    18301 ttgatggcgt cgtgaatgac ccaatgaaga tgggcggtgt cgatttacgg cttaaatttt
    18361 ctggcgattc actgggtgat ctctatgaac tgacgggcgt tctgctgccc gataccccgc
    18421 cgtttgaaac ggatggtcgg ctggtagcga aaatcgacac tgaaaaatcg tcggtctttg
    18481 attatcgcgg ttttaatggg cgaattggcg atagcgatat ccacggttct ctggtctaca
    18541 ccaccggaaa gccacgacca aaactggaag gtgatgtcga gtcgcggcaa ttgcggctgg
    18601 cggacctggg accgttgatt ggcgttgatt ccgggaaagg ggcagaaaag tcgaaacggt
    18661 ctgaacagaa gaagggcgaa aaaagcgttc agcctgcggg caaagtgctg ccttatgacc
    18721 gcttcgaaac cgataaatgg gacgttatgg atgccgatgt tcgcttcaaa gggcggcgca
    18781 ttgagcatgg cagtagcctg ccgattagcg atctttctac tcatatcatc ctcaaaaatg
    18841 ctgacctgcg cctgcaaccg ctgaaatttg gcatggcggg cggcagcatt gcggcgaata
    18901 ttcatctgga aggcgataaa aagccgatgc aggggcgggc agatattcag gctcgtcgac
    18961 tgaaactgaa agaactgatg cccgatgtgg aactgatgca gaagacgctg ggggaaatga
    19021 acggtgacgc ggaactacgc ggtagcggta actcggtggc ggcgctttta ggcaacagta
    19081 acggcaacct gaaactgttg atgaatgacg ggctggtgag ccgcaacctg atggagattg
    19141 ttgggctgaa tgtcggcaac tacattgtcg gtgcgatatt tggtgacgat gaggtgcggg
    19201 tgaactgcgc ggcggcgaat ctgcatattg ccaacggcgt ggcgcgcccg cagatttttg
    19261 ctttcgatac tgagaacgcg ttgattaatg ttaccggcac ggcaagtttt gcttcggaac
    19321 agctggattt gactattgat ccggagagta aagggattcg gattatcaca ctgcgttcgc
    19381 cgctgtatgt gcgtgggacg tttaaaaatc cgcaggctgg ggtgaaagcc ggaccgctga
    19441 ttgcccgtgg tgctgttgct gcggcactgg caacgctggt aacaccggcg gcggcgttac
    19501 tggcactgat ctcaccttcc gaaggggagg ctaatcagtg tcggacgata ttgtcgcaga
    19561 tgaagaagtg atgataattg ccggatgtct gacatccggc aattaaacag atttagattt
    19621 ttggcaaagt gtgctttgtt catgccggat gcggcgtgaa cgccttatcc ggcctacaaa
    19681 tccttgtgaa ttcaatatat tgtatggaaa ttcagtcctg ataagcgtag cgcatcaggc
    19741 ttttcacact tattacaacg actgatgtcg cgtctcatgg gtcagcagca gggcgattaa
    19801 cgtcaagcca gccatcgccg ccagatataa ccccaccgca cctaacccgt agttagcctg
    19861 caaccaagct gcgatatacg gcgcaacgga agccccgaga atcgacgata cgttgtaaga
    19921 gaacgatgct ccggtgtaac gcacttctgt cggaaacagc tctggtaaca gcgcacccat
    19981 cggcccgaag gtcagcccca tcagacttaa ccccagcagc aggaaggcaa aaaccagaat
    20041 cgggttgcca gaaccgagca gcgggttaaa ggcgaacagc gcgaacagga tgatcagcgt
    20101 ggtgatgatc accatgcttt tacggcgacc aaaggcatca gccagtaatc cggcgactgg
    20161 caccatcacg ccaaaaccaa taactgccat catcagcatc cacaacactt cgttacgcgg
    20221 caggccaagc ccaactggcg cggcggcggt actgaaggtc atagagtaga ccgtcatgat
    20281 gtaaaacagc gtataggttg ccagcataat gaacgtaccc agtacggtta cgcgaacatg
    20341 tttggtcagc agcgtaccca gcgggatctt cacctgtttt ttcgctttag cgactttctc
    20401 aaacaccggc gactcatgca gcgacacgcg aacatacagg ccgataatga ccagcaccgc
    20461 cgagaagata aatggcacac gccagcccca gctcataaac tgctcatcgg tcagtagcca
    20521 ggaaagcagc aagaaagtgc cgttggcaaa gaagaagcca atcggtgcgc ctagctgtgg
    20581 gaaggagcca tacagtgcac gtttgcgcgg tggggcgttt tcagtcgcca gcagcgccgc
    20641 gccgccccat tcaccgccta agcccagacc ctgaccaaat cgagccagcg ccagcagcag
    20701 tggggcgaaa ataccaatcg tggcatagcc cggcagcaga ccaatcacca cggtcgaaat
    20761 ccccatcgtt agcaacgagg cgaccagcgt cgctttacgc ccaacgcgat cgccaaaatg
    20821 accaaaaacg gcagagccaa tggggcgcgc gacgaaggcg atggcgaagg tggcgagcga
    20881 ctgtagcgtt gctgccgttg gatcgccctg cgggaagaag atatgcggaa acacaataac
    20941 ggccgcagtg gcgtaaatgt aaaagtcgaa gaactcaatg gccgtgccaa tgagagaggc
    21001 gacaaggact ttattacgcg agttgatcgg cgtgtattct tgttcgtggt cgagtgttgt
    21061 ggctgttgct tgcataatct tttcttattt tttggctaac gaatagccat attacgcaca
    21121 gcaaaagtga tatttcaatg tgtaacaaat cgcggaaatg gtcaaaaacc aggcaaaaag
    21181 gggataatgt ttaggccagt gatttttctt ctatgaaatg cttcacataa ttttataaat
    21241 agtggataaa actggtttca ggttaaataa aagttaccaa ctggatttat atgctggggt
    21301 tatgagttgg agtgaaattg tttgctccca aatagatggc tgaagaggct ctggggaaga
    21361 gtttcaagag gttatgtgcg cattatagcc ggatgcgatg ctggcgcatc ttatccggcc
    21421 tacggggagc caggccgaat aaaatcgtat gccggataag gcgtttacgc cgcatccggc
    21481 ctattttaag gctgcgtttt ccctggcatt cgcgggtcgt ctttatattc ggcggtggca
    21541 atccacgccg cgcaaaacag cgtcaaacgg gcgaagaagt agaaaaatgc catcagcccc
    21601 agcacggaac caaatgctgc gccagagggg gatttcatca gcgatggcag ggtgtaggtc
    21661 atcacgattt taatcacttc aaaaccaata gcagccagaa atgttccgcg aatcagggct
    21721 tttttgcgcg gacggtggcg cggcaaccgc cagaagatcc agaagaaaag cagatagttg
    21781 gcgaagatgg aaatcgccag gccaatcaat cgccacgtcg gtttcagcca ctcaatgctg
    21841 ttcaggtgca gggcgctaat aatcatttgc tgcgccgaac cggcaaccga ggtgatcgaa
    21901 agcgtcacaa tcagcgcaat caacaaacca atcagtgaaa taaaatcacg cagatatttt
    21961 acccagaact tctcctgatc ttgcggcgag cgttcccaga catcgcgcga ctgggcacga
    22021 atcgcttcac gcaggttacc catccagttg atgccggaat aaagtgccac cgccaggccg
    22081 acaagcccta cagtcgtacg ctgctgaacg gcggtgttga tggtgttttt caacgtggcg
    22141 gctagtgtcg gatcgctgat gttttgcaga attttgtcga agatatcctg tagcaacatc
    22201 ggatgggagg ccagcacaaa gccccctgcg gcaaacgaca ccatcaaaat cgggatcatc
    22261 gacaaaaatg agaaataggt gatagccgca ccaaactggt tacccagccg atcgttaaag
    22321 cgttctgtcg cgcgaataag gtgcgcaata accggttggc gttggacttt ttcggcagtg
    22381 gtggtgacgg tttccagcgc ctggctaact ttgccgcctt tctcgctatt atccaactgc
    22441 ttaatcggct cgtgctccag atcctgggtg ggacgtttga tctcgttttc ctgcgtcatc
    22501 cgctcttttg tccttctgtc gtggtcattc ttaaaaagta tagtcggtca gtccaggtgc
    22561 tctttcatta cgccagccag ccactgcata aacaggttta cacgccggga aagctcccga
    22621 cgctgcgggt aaaccagcga aagggagagc ggctcggcac ggtagcctgg caacacttca
    22681 ataagccgcc cggcacgcag ggcttcgcgc acggcaatgc gcgggatctg aataatcccc
    22741 agaccggcaa gaccggcggc gagatagttt tcgctactgt ttaccgtcaa catgccgcca
    22801 gacttaaacc actggacgcc attaacgctg gcaacctcaa aacctaacgg atgtacaccc
    22861 aggtgcggtg tataacgcac tattgcgtgt gaagtcagat catcggggct ttgcggataa
    22921 ccaaagcgcg tcagatagtg cggactggca cagttgacca tggttagttt gccgagggga
    22981 cgggcgataa cgccatcttc cggtaacgcg ccagtgcgta tcacgcaatc aaaaccatca
    23041 tgaagaatat ctaccggacg gttatggcta ctcagttcca gctcaattcc cggatgctga
    23101 tagagaaatt ccgacagacg cggcagtaac aggtttttcg cgattcctga cgggatgtcg
    23161 atgcgtaatt taccgctgat actggtggca tcctgttgaa acagaccgtc cagttcgctg
    23221 agattactca acacatcttt tgcccgttga taataggtca tgccttctgg cgtgagtttg
    23281 acccgtcgcg tagtgcgctg gagaagctgg gtgccaagtt gatgttccag tgcctgtatc
    23341 tggcgcgaaa cacttccctt tggcaaagca aagaaatccg ccgcgcggga aaaactttcc
    23401 agctccgcga ctttgatgaa caactgcatt gcgtgaattt tatccatttc tttgaccgat
    23461 tgttgttcac agcgaaacag taacgcgcat atcgcattgt ttattgtttt ttctaacatc
    23521 taatgccggg tttcacaatg ttgtcatgac gaatgtggcg atttctcagc aggaaaaggg
    23581 cggaatagct ttatgagaaa ggtgaatccc cttaaacaga gcgtattttt cagtgattcc
    23641 ctttaaagtg aatagaggac gtattggcta tttgtgctta tttttgtctc attgccattt
    23701 gttattttgg cttgtagtcg cggaatttca tttttaattg catgcgtact aaccaccaat
    23761 ataaggaaat cctcaaaatt gttttaaata gtgccctcga catgtaattt caggcgttta
    23821 gctgtaacta tttatatcgg taactatttt ctacgcgtca tctctgtaga atggccaccg
    23881 ccaaatggcg aattcttatt ttttctcgcg ggtaataccc ttccactact taagtctttt
    23941 taacgtattc aggtggagat tcagttatgc aaatagtcat gtttgacagg cagtcaatat
    24001 ttattcatgg aatgaaaatc agtttacagc agcgtattcc aggagtgagt attcaggggg
    24061 tcagtcaggc agacgagtta tggcaaaagc tggaaagtta ccctgaagcc ttagttatgc
    24121 tcgatggcga tcaggatggt gagttttgct actggttgct gcaaaaaacc gtggtgcagt
    24181 ttcctgaggt taaggtgtta atcacggcga cggattgcaa taaacggtgg ttacaggaag
    24241 ttatccattt taatgtgctg gccattgtgc ctcgcgattc aaccgtcgag acatttgcgt
    24301 tggcggtaaa tagtgcggcg atggggatga tgtttctgcc tggagactgg cggacgacgc
    24361 cggaaaagga cattaaagat ctcaaatcgt taagcgcccg ccagcgggag attttaacca
    24421 tgttagcagc gggcgaatca aataaggaga tcggcagagc gctgaatatc agtaccggaa
    24481 cggtaaaagc gcatctggaa tccctgtatc gccgtctgga agtgaaaaat cgcacccagg
    24541 cggcaatgat gttaaatatc tcctcctg
//
LOCUS       DAETPZ010000057        24188 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.057, whole genome shotgun sequence.
ACCESSION   DAETPZ010000057 DAETPZ010000000
VERSION     DAETPZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.057"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(22..594)
                     /locus_tag="K0W59_002588"
     CDS             complement(22..594)
                     /locus_tag="K0W59_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HBI3023576.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="K0W59_002589"
                     /pseudo
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="K0W59_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="K0W59_002590"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="K0W59_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HBI3023577.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="K0W59_002591"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="K0W59_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBI3023578.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="K0W59_002592"
     CDS             complement(3556..5337)
                     /locus_tag="K0W59_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBI3023579.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYNTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="K0W59_002593"
     CDS             complement(5330..7102)
                     /locus_tag="K0W59_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBI3023580.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSELVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIHEEAVDA"
     gene            complement(7132..7590)
                     /gene="decR"
                     /locus_tag="K0W59_002594"
                     /pseudo
     CDS             complement(7132..7590)
                     /gene="decR"
                     /locus_tag="K0W59_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="K0W59_002595"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="K0W59_002595"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HBI3023581.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLSSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8662..10361
                     /locus_tag="K0W59_002596"
                     /pseudo
     CDS             8662..10361
                     /locus_tag="K0W59_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10426..11121
                     /gene="queC"
                     /locus_tag="K0W59_002597"
     CDS             10426..11121
                     /gene="queC"
                     /locus_tag="K0W59_002597"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HBI3023582.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11173..11571)
                     /gene="fadM"
                     /locus_tag="K0W59_002598"
     CDS             complement(11173..11571)
                     /gene="fadM"
                     /locus_tag="K0W59_002598"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HBI3023583.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11665..12036)
                     /locus_tag="K0W59_002599"
     CDS             complement(11665..12036)
                     /locus_tag="K0W59_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBI3023584.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12187..14058)
                     /gene="ppiD"
                     /locus_tag="K0W59_002600"
     CDS             complement(12187..14058)
                     /gene="ppiD"
                     /locus_tag="K0W59_002600"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBI3023585.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNETESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14250..14522)
                     /gene="hupB"
                     /locus_tag="K0W59_002601"
     CDS             complement(14250..14522)
                     /gene="hupB"
                     /locus_tag="K0W59_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HBI3023586.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14731..17085)
                     /gene="lon"
                     /locus_tag="K0W59_002602"
     CDS             complement(14731..17085)
                     /gene="lon"
                     /locus_tag="K0W59_002602"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBI3023587.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17273..18547)
                     /gene="clpX"
                     /locus_tag="K0W59_002603"
     CDS             complement(17273..18547)
                     /gene="clpX"
                     /locus_tag="K0W59_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HBI3023588.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18673..19296)
                     /gene="clpP"
                     /locus_tag="K0W59_002604"
     CDS             complement(18673..19296)
                     /gene="clpP"
                     /locus_tag="K0W59_002604"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBI3023589.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19543..20841)
                     /gene="tig"
                     /locus_tag="K0W59_002605"
     CDS             complement(19543..20841)
                     /gene="tig"
                     /locus_tag="K0W59_002605"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBI3023590.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21185..21502)
                     /gene="bolA"
                     /locus_tag="K0W59_002606"
     CDS             complement(21185..21502)
                     /gene="bolA"
                     /locus_tag="K0W59_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HBI3023591.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21807..22385
                     /locus_tag="K0W59_002607"
     CDS             21807..22385
                     /locus_tag="K0W59_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBI3023592.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22429..23904
                     /gene="ampG"
                     /locus_tag="K0W59_002608"
     CDS             22429..23904
                     /gene="ampG"
                     /locus_tag="K0W59_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HBI3023593.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5670 a         6659 c         5833 g         6026 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacggtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattg cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgctag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg aagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccaga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcactc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccgctgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcccacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataatagtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctca gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacgttgg cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcaa gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gttgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gagatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact acgcctactg ccgcccgatt aaacagcagc gactgagcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gtgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccagccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgagttcgct accatcgttc accaccggcg cttccgccag catcgcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cagcaatcag caccatggtg gcgatccaca tcaggatcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctagacgcgc atcagataat cgtattcacc agccatgcgc cagaacccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtcagcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ctgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcgttcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctgc cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 ataatattgt tccgggttta tatgcgattg ctcaaccgtc ttaaccagta tcaacgtctg
     8701 tggcaacctt cagccggaaa gccgcaaacc gtcaccgtca gcgaactggc cgaacgctgt
     8761 ttttgcagcg aacgccatgt tcgtacgctg ttgcgtcagg cacaggaggc gggatggctg
     8821 gagtggcagg cgcagtcagg acgcggaaag cgcggacaat tacgctttct ggtcacgccg
     8881 gaatcgctac gcaatgcgat gatggaacag gcactggaaa ccggaaagca gcaagatgtg
     8941 ctggagctgg cgcaactggc cccaggtgag ctgcgcactc tgttacagcc gtttatgggc
     9001 ggacaatggc aaacgataca cccacgttgc gtattcccta ctatcgcccg ctcgaaccgc
     9061 tacaaccagg ctttttgccc ggccgtgccg agcagcatct cgccgggcag atattttccg
     9121 gcctgacccg cttcgataat aatactcagc gcccgattgg cgatttagcg catcactggg
     9181 aaacctctac tgacgggtta cgctgggact tttatcttcg ttcaacccta cactggcata
     9241 acggcgatgc agtaaaagcc tcacacttac accagcgatt attgatgctg ttacaactgc
     9301 cagcactgga tcaattattt attagcgtga agcgtattga agtcacccat ccgcagtgtc
     9361 tgaccttctt tttacatcgc cccgattact ggcttgcgca ccggctggcg agctattgca
     9421 gccatctggc gcatccgcaa ttcccactga tcggcactgg tccttttcgc ttaacacaat
     9481 tcacagcgga gctggtgcgc ctggagagcc atgattatta ccatttgcgt catccgctgc
     9541 ttaaagcggt tgagtactgg ataactccgc cgcttttcga aaaagatttg ggaaccagtt
     9601 gtcggcatcc cgtgcaaatc accatcggca aaccggagga gctgcaacgg gtcagccagg
     9661 tcagtagtgg catcagttta ggtttttgct atttaacgtt gcgcaaaagt ccccgactct
     9721 ccctctggca ggcgcgaaaa gtgatctcca ttattcatca atccggttta ttacaaacgt
     9781 tagaagtcgg agaaaacctg atcaccgcca gtcatgcatt actgccaggc tggactattc
     9841 cgcactggca agtaccggat gaagtcaaac taccgaaaac cttgacgctg gtttatcacc
     9901 taccgataga acttcatacc atggcagaac gcctacaggc gacactggca gcggaaggct
     9961 gtgaactcac aattattttt cataacgcaa aaaactggga cgacacgacc ctactggcac
    10021 acgcagacct catgatgggc gacagattaa ttggcgaagc accggaatat actctggagc
    10081 aatggctacg ttgcgatcca ctatggccac atgttttcga cgctccagca tatgcacatc
    10141 tgcaatcgac gctggacgcg gcgcaagtaa tgcctgatga ggaaaaccga tttaatgccc
    10201 tgaaagcggt ttttagccag ttaatggcag acgcgacgct gacgccgctg ttcaactatc
    10261 actatcgcat tagtgcccct cccggcgtga acggtgtgcg actgacaccg cgcggctggt
    10321 ttgaatttac cgaagcctgg cttcccgcgc cgtcgcaatg aatagctggt ccgggttaat
    10381 cgcaggcgtt accataacgc ttttattgtt caagcaggat tatctatgaa acgtgctgtc
    10441 gttgtgttca gtggaggcca ggattccacc acctgtctgg tgcaggcatt acaacaatat
    10501 gatgaagtcc attgcgtgac gttcgattac ggtcagcgac atcgcgcaga aatcgacgtg
    10561 gcacgcgaac tggcgctgaa actgggagca cgcgcgcata aggttcttga tgtaacgctg
    10621 ctcaacgagc tggcggtcag cagcctgaca cgtgacagca ttccggtgcc tgattatgaa
    10681 cctgaagccg atggcatccc gaatacgttt gtcccagggc gtaatatttt gttcctgacg
    10741 ctggcggcaa tatatgcgta tcaggtaaaa gcagaagccg taattaccgg cgtctgtgaa
    10801 acggatttct ccggctaccc ggattgccgc gatgagtttg tgaaagcact aaaccatgcc
    10861 gtcagtttgg gcatggcgaa agatattcgt tttgaaacgc cgctaatgtg gattgataaa
    10921 gcggaaacct gggcgctggc agattattac ggcaaactgg atttagtccg taacgaaacg
    10981 ttgacctgct ataacggcat taaaggcgac ggttgcggtc attgtgcggc atgtaattta
    11041 cgcgccaacg gtttgaatca ttatctggcc gataaaccga cggtgatggc agcgatgaag
    11101 cagaaaaccg ggttgaggta attattccgg gtgtcgccgg atgcggcttg agcatccggc
    11161 accacaaaac gtttacttaa ccatctgctc cagcttttcg cgcaattccc cttccagagc
    11221 taatgctttc tgcgttttaa gatcaataca aacaaacgta ataagcgcat ccgctaccac
    11281 ctgcccttcc ggctccagtg taatgacctg gcttaagatg ccgcttttac cgtttaattg
    11341 ctgcaactga ctggtaatag ttaacaggtc gcttaatacc gctgggcgac gatagttaat
    11401 attgatattg accacgacga aggcgatgtt atgggccgtc atccactgaa aactgtcgct
    11461 attttccaac ccatcccagc gggcttcttc gagaaattca aggtagcggg cgttgttgac
    11521 gtgctggtaa acgtcgagat gatatccacg aactttgatt tgtgtttgca tagcgcaata
    11581 accttacgtt gttgttataa gtacagaggt cataactggt atgacctctt cagtctggca
    11641 aaaaattgcc actatgcaaa ttaattacag ggttaatacc gccagattac gttccaccag
    11701 cgaattgccc atccccggca cctgctttag atcctccacc gttttaaacg gaccgtactc
    11761 ttcacgataa ctgacaatcg cctgcgcttt cttcaggcca acgccattca tcgcgcgggc
    11821 tagctcttcc gcgctggcat tattaatgct gacccgggtg ccttcttcgt cactggcttt
    11881 cgccggtact gctgctttac tttgtgctgc aggagcttcc gctttggttt ctaccgccgt
    11941 cggtttcgcc acagaagctg ccgccagcgc gctatgagac attccggcac aggccaggga
    12001 cagggtaatg agcagtgctt taattccgtg tttcatactg ttttctcctt gtttgttaac
    12061 agtggaatca ccttaacggg agtgaaaaca gtaaacaaat ggcaatgttc agaaatggaa
    12121 aaggccgcga aagcgacctt ttcttgttac agctgcaacg catttttttg cgaggcggct
    12181 tcaggattat tgctgttcca gcgcatcgcc aattttgatt ttcgcctctt tacgcaggtt
    12241 actcatcaga gcttcaaaga cgatttgtgc gttgttctgg gtgataccct gcaccatcgc
    12301 ttttttctga tcttccggca ttgaaccttg tttcacttca tccagcgcca gcagaaccac
    12361 attaccttgc atatcggtcg ccataccgta gctcggtttg tctttcgctg gcagtggcag
    12421 tgcaaacgcc gcctggctaa tcgggtcacg accggaacgg cttaaggttt tcggctcgcc
    12481 aaatttcaga ccggcagcct gcatagcttc cgcacctttg ccggctttca aatcaaccag
    12541 cagtttctca gcatccactt tcgcctgttg ttcagctttg ttgtgctgaa ccagtgcctt
    12601 aacttgttcc tgaacatctg ccaacggttt caccgcttcc ggtttgtgct cgctgacgcg
    12661 cagcacgaat gcgcggtcgc cgtctacggt gatgatgtca gagttgatgc ccgacgcgcc
    12721 gttttcacct accagaccgc cgttaaagat agcgtcggca accggcttga agttcaactc
    12781 ttccggcagg ttatctttgc tgaaccaacc cgtctgagtg gctttaacgc cggcagcttg
    12841 ctctgcaccg gccagagact cggtttcgtt gcttgccgca tcgctcactt tctgctgcag
    12901 cgcatagtac gcatcgaggg ctttttcgtg tttcactttc gccgcaatgt cgtcacgtac
    12961 ttcgtctaac gatttcactt tcgctggctg aatgtcgtcc agacgtacaa tcaggaaacc
    13021 gaccgaagat ttgatgacac cagacagttg gcctttttct ttcagaccag catttttcag
    13081 ttcgtcgggg atagtggcat cttctaacca acccatatcg ccgccgttac gggcagagat
    13141 aatatcggca gatttttctt tggctaatgc agcaaaatca ccgcctttat tcagctcatc
    13201 aagtaccgct ttcgcttcat cttcagtttt ggtctggatg atgctgtagc gggtacgctg
    13261 cggctgggtg aattgatcct gatgctgatc gtagtagctc tggatatccg catcgctaac
    13321 cggttgctgc atcgttgcgg caaccagctt gatgtaactc acgcggaatt gttccggcgt
    13381 catgaaattg tttttgtttt gttcgtagta gctggcaatc tcctgttcgg tcacaggctg
    13441 cttcgccgcc agcgcgttaa catcgatagt cgcctcacgc accacgcgtt gttgcgcgac
    13501 cagtgccgcc agctcgtcgg tttcaccttt cagcataaaa tcggtaccgg caacgccgtt
    13561 aatcagctgt tgggtagtga gctggttacg cagcgcctgg gcgtactgat cggcggtcat
    13621 ccccatctgg ttgaggatac cgttatagcg gctgttatca aatttgccgt caacctggaa
    13681 ggctggggtc gcgaaaatcg cctgtttaac ctgctcatca ctgataccca gtttcagctc
    13741 acgagcgtac tgatccaaca gcgcctcgtc gatcagacga ttcagcacct gttgacgcag
    13801 ggttttcata tagccttcgt tcgctgccag ctcggagtat tgatcgccca gctgttgctg
    13861 catgcgatta cgctcgctgt taaaggcgtt ctcgaactgc ccacggctga tttcctggtc
    13921 attcactttt gcggcgtagt tattgcctcc gccaatcagg taaccactca cgccggtcaa
    13981 tatgaacgac acgataatga taccgaaaat aatcttgagc acgagactgt ttgcagccgt
    14041 gcgtaagctg tccatcatgg tgtaacaaca ctccgctgta gatgactgtg tacctcacgc
    14101 aacgtatcga tgcgcgcaaa ggcctattgt gacaagaaac gggggcaatt gtcagcccac
    14161 aagacacaga aagtcaccga atacaaataa aaaaggcaca tcagtagatg cgcccttgaa
    14221 cttcgtcaca tccccactgg ggacaacgct tagtttaccg cgtctttcag tgctttacct
    14281 gcacggaagc tcggtacttt agcggcagcg atggtgatct ctttaccggt ctgcgggttg
    14341 cggccagtac gggcagcacg ctctttaacg gcaaaagtac caaaacctac cagtgctaca
    14401 tcatcccctt ctttcagaga ttcagttacg gaagcaataa tagcatctaa cgcacggcca
    14461 gccgcagctt tagagatatc agcccctgca gcaatcttgt cgatcaattg agatttattc
    14521 actcttctct tcctctttat aatttatatc gcacttgaat ccttcaaggt acgaacgcgc
    14581 agcagttata tcaggccagc catcccctta caacacccct taataagggc aagcccgatc
    14641 cgctatctaa cttagcgaga caaaaaaagg ctggcaagcc cgaattagcc tgccagccct
    14701 gtttttatta gtgcattttg cgcgaggtca ctattttgca gtcacaacct gcatgccaga
    14761 cggttcattt tgcagcgcca gagtcagaac ttcctcaatg cgcttcacag gatgaatgtc
    14821 cagatcggca attacgttgt caggaatctc ttccagatcg cgtttatttt cgaacggaat
    14881 tagcactgtt ttaatcccgc cgcgatgcgc tgccaggagt ttttctttca aaccaccgat
    14941 cggcagtacc tgaccacgca gagtgatctc accggtcatt gccacatcgg cacgaaccgg
    15001 gttaccggtc aggcaagaaa ccagcgcggt gcacatagca ataccggcac tcggaccatc
    15061 tttcggcgtc gcaccttccg gtacgtggac gtggatgtca cgtttttcgt aaaaatcagg
    15121 gttgatcccc agtttttccg cacgcgcacg aaccaccgtt aacgccgcct gaatagactc
    15181 ctgcatcact tcgccgagcg aaccggtata ggtcagtttg cctttacccg gaacacatgc
    15241 ggtttcaatg gtcagcaagt caccgcccac ttccgtccac gccagaccgg ttacctgacc
    15301 gacacggttt tcgttatcag cgcgaccata gtcgaaacgc tgaacaccga ggtagtcatg
    15361 caggttatcg ccgttaattt cgatatgttt taatgactta tcgagcagta actgcttaac
    15421 cgctttgcgg cacagtttgg agatttcacg ctccagacca cgcacgcccg cctcacgggt
    15481 gtagtaacga ataatgccga taatggcgct atcgtcgacg gtcagctcac cttttttcag
    15541 tgcattacgt tcaatctgct tcggcagcag gtgacgtttg gcgatgttca gtttttcatc
    15601 ttcggtataa ccggagaggc gaatcacttc catacgatcc agcagcggtg ccggaatgtt
    15661 catggagttc gacgtcgcga caaacatcac gtcgctgaga tcgtaatcca cttccaggta
    15721 gtggtcgctg aacgctacgt tctgctctgg atccagcact tcaagcagtg cagaggccgg
    15781 atcgcctcgc atgtcagaag acattttgtc gatctcatcg agcaggaaca gcgggttttt
    15841 cacgcccact ttcgccattt tctggatcag tttacccggc atagaaccga tgtaagtacg
    15901 gcggtgacca cggatttccg cttcatcacg cacgccgccc agcgccatac ggacatattt
    15961 acgcccggtg gctttggcaa tggactgacc aagagaggtt ttacctaccc ccggcggccc
    16021 taccaggcag aggatcggtc ccttgatttt gttgacacgg ctttgaaccg caaggtactc
    16081 aaggatgcga tctttcacgc gctccagacc ataatggtcg gtatcaagga tttcctgcgc
    16141 ctgacgcagg tcttttttga ccttgctgcg cgcattccac ggtacctgta ccatccagtc
    16201 gatataacca cgcactacgg tcgcttctgc cgacatcgga gacatcattt tcagcttctg
    16261 caactctgct tccgctttct cttttgcctc tttcggcatc ttcgccgcgt cgattttgcg
    16321 cttcagggct tcgttttcgt ccggcgcgtc gtccatttca ccgagttctt tctgaatagc
    16381 tttcatttgc tcgttcagat agtactcacg ctgggatttc tccatctgct ttttaacgcg
    16441 gttgcgaatg cgtttctcaa cctgcagcag atcgatttcc gattccatca ttgccatcag
    16501 atattccaga cgttcgttaa cgtcggacat ctccagaacg gactgtttgt cagccagttt
    16561 cagcggcata tgtgcagcaa tggtatccgc cagacgcgcc ggatcgtcga tgctattcag
    16621 cgacgtcagc acttctggtg ggattttttt gttcagcttg atgtagcctt cgaactggct
    16681 gattgcagta cgcaccagca cttcctgttc ccgctcatca atggtcggcg actccagata
    16741 ctccgccttc gcagaaaagt gttcgccatt gtcagagagc gcagaaatac gcgcgcgctg
    16801 taacccctcg accagcactt tgactgtgcc gtcaggcagt ttcagcatct gcaatataga
    16861 ggccacggtc ccgacggtga aaagatcgtt tacacccggc tcatccgttg aagcttcttt
    16921 ctgcgcgacc agcataattt ttttatcatg gtccatcgcc gcttccagac aacggataga
    16981 tttttcccgc ccgacaaata aggggatgac catgtgcgga taaaccacca catcgcgcag
    17041 cggcaatacg gggatttcaa tgcgttcaga acgctcagga ttcatagagc tctctcttag
    17101 tttaatttcc gccaggtaat cagatgacac gactgtgctt cacgccatct attaacatgt
    17161 acgtcagtat atggggatgt ttcccccaca ttcaacgccg agaatagagg aaaaattaaa
    17221 ggggagataa aatcccccct ttttgtttaa ctaattgtat gggaatggtt aattattcac
    17281 cagatgcctg ttgcgcttcc ggcttaccat aaatcagcaa cggtttgctt tgaccatcaa
    17341 ttaccgactc gtcaataacc actttttcga cgtcttccat ggacggcaga tcgtacatgg
    17401 tatcgagcag tgcggcttct acgatggaac gcaggccacg ggcaccggtt ttacgcgcca
    17461 tcgctttctt agcgatagca tccagcgcct cgtcacggaa ttccagatcc acgccttcca
    17521 gattaaacag cgcctgatac tgcttggtca gggcgttttt cggctctttg aggatctgaa
    17581 tcagagcttc ttcgctcagt tcattcaacg ttgctacaac cggcagacga ccaatgaact
    17641 cagggataag accaaacttg atcagatctt ccggttcaac ctgcgccagc agctcgcctt
    17701 cgcttgcttt gtcggacttc gcttttaccg tcgcgccaaa accaatgccg gagccggttt
    17761 ctacacggtg ggaaatcact ttatccagac cggcaaacgc accgccacaa ataaacagga
    17821 tcttagaggt atcaacctgc aagaattcct gctgcggatg tttacgccca ccttgcggtg
    17881 gaacagcagc taccgtacct tcgatcagtt tcaacagtgc ctgctgtact ccttcaccgg
    17941 aaacgtctcg ggtaatggac gggttgtctg acttacgaga aatcttgtcg atttcatcga
    18001 tgtagacaat cccgcgctgc gctttctgta cgtcgtaatc gcacttttgc aacagcttct
    18061 gaatgatgtt ttcaacgtct tcgcccacat aaccggcttc ggtcagcgtg gttgcgtcgg
    18121 ccatggtgaa cgggacgtcc agcaggcgcg ccagcgtttc agccagcagc gttttaccgg
    18181 aaccggtcgg accgatcagc agaatgttac ttttgcccaa ctcgacacca ttgctggtat
    18241 cgccgttgcg cagacgtttg taatggttgt ataccgcgac cgccagcact tttttcgcct
    18301 gttcctggcc gataacgtaa tcgtccaggt ggttgcgaat ttcatgcggc gtcggtagcg
    18361 cactgcgttc acgatgcggt gcaacttctt taatctcttc gcgaatgatg tcgttacata
    18421 aatcaacaca ttcgtcgcag atatacacgg atggaccggc aatcagcttg cgcacttcat
    18481 gctggctttt gccgcaaaaa gagcaataca gcaatttgcc tgagccatct ttgcgtttat
    18541 ctgtcatggg tcaaaacctc ttctttgttc tttgtgccgc acacgacgac gcaaatgcca
    18601 ttctcaggcg caagccgctt acagcgttgt gccgccctag ataagtatag cggcacagtt
    18661 gcgcctctgg catcaattac gatgggtcag aatcgaatcg accagaccgt attccaccgc
    18721 ttcaggggcg gaaaggaagc gatcgcgctc ggtatcacgt tcaatctgtt ctaatgattg
    18781 acccgtatga agcgccataa gttcattcat gcgcccttta actttcagaa tttcacgagc
    18841 atgaatttcg atatcggtcg cctggccctg gtagccgccc aacggttggt gaatcatcac
    18901 gcgcgaattc ggcaggcaaa aacgtttacc ttttgcccct gcggtcaaca agaaagcgcc
    18961 catcgaggcc gcctggccca tacagatggt gctgacatca ggcttgataa actgcatggt
    19021 gtcatagata gacatcccgg cagtgatcac cccgcctggg gagttaatgt acagatagat
    19081 atctttttct gggttttccg cttccaggaa cagcatctgc gccacaatca ggttagccat
    19141 gtggtcttca acctggccag tcagaaaaat gacgcgttcc ttaagtagac gagaatagat
    19201 atcaaaagag cgctcaccgc gtgaggtctg ttcaatgacc atcggcacca gcgccatatg
    19261 gggtgcaaag ttatctcgtt cgccgctgta tgacatttcc gtctcctgga taaaattgaa
    19321 aaaaacctgc tgtaccgatt gtaaccttat ggacggatta tcagctagtc cctgtgttat
    19381 gggtacggca tcaccatatt tcaagcataa caatcttttg ttgttacgct aacaccgaaa
    19441 gaggcttttc gcacggctcc atgcaaaatt catgacaaaa aaaagcccac cacctggcgg
    19501 tgacgggcct ttgtgcgaat ttagcgcgtt atgctgcgta aattacgcct gctggttcat
    19561 cagctcgttg aaagtggttt ctttttcagt cactttcgct ttcgccagta cagcttcaac
    19621 agcctgttct tccagagcaa cattgcgcat gttgtccatc agttctttgt ttttgctgta
    19681 gaactcgata acttctttcg gatcttcgta cgcagaagcc atctcttcga tcaggccttt
    19741 cacgcgctct tcgtcagctt tcagctcgtt ggtgcggata acttcgccca gcagcaggcc
    19801 aacaactacg cggcgtttag cctgttcttc gaacagttcg cgcggcagtt ccagagcttg
    19861 tttttcgttg ccaccgaaac gctgtgcagc ctggcgacgc agaacgtcga tttcgctgtc
    19921 gatcagcgca gccggtacgt cgatgtcgtt agcttttacc agaccttcga tcgcctgaga
    19981 cttaacgcgg ttacggatgg cgctcttcag ctcgcgctcc atgtttttac gcacttcagc
    20041 gcgcagacct tctacggaac catcttcaac gccgaaacgt ttgatgaatt ctgcagtcag
    20101 ttccggcagt tcacgctctt caactttctt caggttgata gcgaatttcg ctgctttacc
    20161 tttcaggttt tctgcgtggt attcttccgg gaaggtcacg tcgatggtga actcttcgcc
    20221 agctttgtgg cctttgatac cgtcttcaaa gcccgggatc atacgaccct ggcccatcgc
    20281 cagtacgaaa tcagacgctt taccgccttc gaactcttcg ccgtctacag aaccggtgaa
    20341 gtcgatggtt acgcggtctt ctgcttcaac agcgccgtct ttttctttcc aggtcgcctg
    20401 ctgtttacgc agagtatcca gcatgccgtc aacgtcagcg tcggtcactt caacgatcgg
    20461 tttttcaact tcgatcgctt ccagaccctg cagttcaact tccggataaa cttcaaactc
    20521 tacagagtaa gtgaagtctt cacccagctt gtattcgccc ggaacataag tcggtgcgcc
    20581 agccggattg attttttctt taatgatggc gtcaatgaag ttacggctca tcaggtcacc
    20641 cagaacgtcc tggcgtacag acgcgccata acgctgagca acgatattca ttggcacttt
    20701 gcctttgcgg aagccgtcaa tacgtacttt tttcgcaacg ttgaccagct cgcttttaac
    20761 agcggtctcg atgctgtcag cagcgatagt aatcgttaca cggcggccaa ggccttgagt
    20821 ggtttcaact gaaacttgca tcttgttacc tcaaaaaatc acagtgctcg gtcaactctt
    20881 accccgcaag cacaggttgt tggcttcgcg gaaccgggat gttcttataa tcaatcacat
    20941 tccctgttac cagaatcatc ccgaaagcat tcaaatagga cgcggcatta tagcggcatc
    21001 acttttatga gtcgagaacg gttattgcgc gctgtcgcgt attttttcac aattcccggg
    21061 caattttcgt ctgaatgctg ggcaaaaagc gcaaaatcca gacaaaacaa aacggcccgc
    21121 aggccgtttt tcataaaatc tttagcaaca tactggaaaa gcgccgacag ttgcaaatgc
    21181 gtttttacgc gatgcttcct gctccacgac agggaggaga ggcaaagacg gtgtcctgca
    21241 acccttccca ctccttaata gtgtaagtat gcagagccag cgcatgaacg gtagtagaga
    21301 gttcctccgc taaagtactg taaatcattc gatgacgatt cagaaaacgt tcacccgtaa
    21361 aacgatcgct gaccagcaca actttaaaat ggctttcaga gccggctggg acattgtgac
    21421 gatagctttc atccactact tcgaggaata cgggttggaa cgccgccctt aatttttctt
    21481 ctatccgctc acgtatcatc atgaaatatc tcctccgaca acgctgagat gtcatccatc
    21541 cctttaaata ctagccgctt ttaccgtttc cattgcagct taacaacaaa tatttaggtt
    21601 tcgtcctggt ttttccgtca agcgtatgaa gtttactgat ttaaagattg aaacacaggc
    21661 ctcgcatcag cgtttctttt accaggggtg attatttcgt caacatgatg aatttacatg
    21721 ccttacccac ttcccctcgc cgttggcaat gttatgatgg cgggaatttt taccaaacac
    21781 gcttaagagt ctctgagagc ccgaacatgt tcaaaaaaat cctcttcccg ttagttgctc
    21841 tgtttatgct tgcaggatgc gcaaaaccgc caacaactat tgaagtttcc ccgacgatta
    21901 cactgccaca gcaggatcca agcctgatgg gcgtcaccgt aagcattaat ggtgccgatc
    21961 agcgtacaga tcaggcgctg gcaaaagtca cccgcgataa tcaaatcgtt accctgaccg
    22021 cctcccgcga tctgcgtttc ctgctgcaag aagtgctgga aaaacagatg accgcgcgtg
    22081 gttacatggt tggtccgaat ggcccggtta atctgcaaat catcgttagc caactgtatg
    22141 ctgacgtgtc ccagggcaac gtgcgctaca acatcgctac caaagcggat atcgccatca
    22201 tcgctaccgc gcagaatggc aacaaaatga ccaaaaacta tcgtgccagc tacaacgttg
    22261 aaggtgcgtt ccaggcctcc aacaaaaata tcgccgatgc ggttaacagt gtgctgaccg
    22321 ataccatcgc tgatatgtct caggacacca gcattcacga attcatcaag cagaacgcgc
    22381 gttaatttct gccctctggc ccggtgcaag ccgggcctgt agacgcccat gtccagtcaa
    22441 tatttacgta tttttcaaca gccgcgttca gccatattgc tgatcctggg ttttgcttcc
    22501 gggctaccgc tcgccctgac atccggcacc ttacaggcct ggatgacggt cgaaaatatc
    22561 gatctcaaaa ccattggttt cttctctctg gtaggccagg cttacgtttt taaattcctc
    22621 tggtcaccgc tgatggaccg ctacacgccc ccattttttg ggcggcggcg cggttggctg
    22681 ctcgccacgc aaatcctgtt attagtcgcc attgcggcga tgggttttct cgaaccaggc
    22741 acccaactcc gctggatggc ggcgctggcg gtggtgatcg ctttttgctc tgcctcccag
    22801 gatattgtct tcgatgcgtg gaaaaccgat gtgctcccgg cagaagaacg tggtgcgggc
    22861 gcggcaatca gcgtgctggg ttaccgttta gggatgctgg tttccggcgg cctagccctg
    22921 tggctggcag ataaatggtt gggctggcag ggcatgtact ggttgatggc ggcgctgttg
    22981 atcccctgta ttatcgcgac gttgcttgca ccagaaccaa ccgacaccat tcctgtgccc
    23041 aaaacgctgg aacaagcggt tgttgcacct ctgcgagatt tctttggtcg caataatgcc
    23101 tggcttattt tgcttcttat cgtgctgtat aagctgggcg acgcattcgc catgagcctg
    23161 acaaccacgt ttttgattcg cggcgtcggg tttgatgcgg gtgaagtagg cgtggtaaac
    23221 aaaacgcttg gcttactggc gaccattgtt ggcgcattgt acggtgggat tttgatgcag
    23281 cgcctgtcgc tgttccgggc gctgctgatt ttcggcattt tacaaggtgc gtctaacgct
    23341 ggttactggc tgctgtcgat tactgataag catctctaca gcatgggcgc agccgtcttt
    23401 ttcgaaaacc tctgtggcgg gatgggcaca tcagcctttg tcgcgctgtt aatgacgctg
    23461 tgtaataagt cattttccgc tactcaattt gccctgctct cagcgctttc cgctgtaggg
    23521 cgagtttatg tcggccccgt ggcgggttgg tttgttgaag cacacggctg gtcgacattc
    23581 tatctattct ccgtcgccgc tgccgtaccg gggcttattt tgctgctggt ttgccgccag
    23641 acgcttgaat atacacgagt aaatgacaac tttatctccc gtaccgaata tccggcaggt
    23701 tatgcctttg ccatgtggac actggcggcg ggcgtcagcc tgttggccgt gtggttactt
    23761 ctgttgacga tggacgcgct ggatttgacg cacttctctt tcctgcctgc tctgctggaa
    23821 gtcggggttt tagtcgccct ttctggcgtc gtgcttggtg gtttgctgga ttatctggcg
    23881 ctacgaaaaa cgcatctgac gtaatctgta aatattattt agctgtagta atcactcgcc
    23941 gtaattatta cggctaaata aacatcagca tcgcttatta atatttgttc ttttgtgcgg
    24001 cttagcgttt ggaaattatt ggcgccattt ataaattcta tttttcttac acgattcagc
    24061 taatgagtct ttattttctc accacccagt tgtcactcta atgataatta tttgttaaat
    24121 aattgtttta tttcgcattg gttataccaa ttgcccgcca agactccttc tgcactcccc
    24181 ttttgtta
//
LOCUS       DAETPZ010000058        23751 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.058, whole genome shotgun sequence.
ACCESSION   DAETPZ010000058 DAETPZ010000000
VERSION     DAETPZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23751
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.058"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            583..2511
                     /gene="thrS"
                     /locus_tag="K0W59_002609"
     CDS             583..2511
                     /gene="thrS"
                     /locus_tag="K0W59_002609"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBI3023594.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2515..3057
                     /gene="infC"
                     /locus_tag="K0W59_002610"
     CDS             2515..3057
                     /gene="infC"
                     /locus_tag="K0W59_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBI3023595.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3154..3351
                     /gene="rpmI"
                     /locus_tag="K0W59_002611"
     CDS             3154..3351
                     /gene="rpmI"
                     /locus_tag="K0W59_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBI3023596.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3404..3760
                     /gene="rplT"
                     /locus_tag="K0W59_002612"
     CDS             3404..3760
                     /gene="rplT"
                     /locus_tag="K0W59_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBI3023597.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    3877..4001
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3883..3927
                     /gene="pheM"
                     /locus_tag="K0W59_002613"
     CDS             3883..3927
                     /gene="pheM"
                     /locus_tag="K0W59_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HBI3023598.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4210..5193
                     /gene="pheS"
                     /locus_tag="K0W59_002614"
     CDS             4210..5193
                     /gene="pheS"
                     /locus_tag="K0W59_002614"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBI3023599.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5208..7595
                     /gene="pheT"
                     /locus_tag="K0W59_002615"
     CDS             5208..7595
                     /gene="pheT"
                     /locus_tag="K0W59_002615"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBI3023600.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARCHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEATEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREVDLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7600..7899
                     /gene="ihfA"
                     /locus_tag="K0W59_002616"
     CDS             7600..7899
                     /gene="ihfA"
                     /locus_tag="K0W59_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HBI3023601.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8000..8980
                     /gene="btuC"
                     /locus_tag="K0W59_002617"
     CDS             8000..8980
                     /gene="btuC"
                     /locus_tag="K0W59_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HBI3023602.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9043..9594
                     /gene="btuE"
                     /locus_tag="K0W59_002618"
     CDS             9043..9594
                     /gene="btuE"
                     /locus_tag="K0W59_002618"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HBI3023603.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYQDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9594..10343
                     /gene="btuD"
                     /locus_tag="K0W59_002619"
     CDS             9594..10343
                     /gene="btuD"
                     /locus_tag="K0W59_002619"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HBI3023604.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10421..10885
                     /locus_tag="K0W59_002620"
     CDS             10421..10885
                     /locus_tag="K0W59_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBI3023605.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11132..11834
                     /gene="ydiV"
                     /locus_tag="K0W59_002621"
                     /pseudo
     CDS             11132..11834
                     /gene="ydiV"
                     /locus_tag="K0W59_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
     gene            11898..13334
                     /locus_tag="K0W59_002622"
     CDS             11898..13334
                     /locus_tag="K0W59_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HBI3023606.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMPMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13338..13529)
                     /gene="hemP"
                     /locus_tag="K0W59_002623"
     CDS             complement(13338..13529)
                     /gene="hemP"
                     /locus_tag="K0W59_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HBI3023607.1"
                     /translation="MRYTDSRKLTLETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13661..14707)
                     /gene="aroH"
                     /locus_tag="K0W59_002624"
     CDS             complement(13661..14707)
                     /gene="aroH"
                     /locus_tag="K0W59_002624"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HBI3023608.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14864..15697)
                     /gene="ppsR"
                     /locus_tag="K0W59_002625"
     CDS             complement(14864..15697)
                     /gene="ppsR"
                     /locus_tag="K0W59_002625"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HBI3023609.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16029..18407
                     /gene="ppsA"
                     /locus_tag="K0W59_002626"
     CDS             16029..18407
                     /gene="ppsA"
                     /locus_tag="K0W59_002626"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HBI3023610.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18464..20110)
                     /gene="fadK"
                     /locus_tag="K0W59_002627"
                     /pseudo
     CDS             complement(18464..20110)
                     /gene="fadK"
                     /locus_tag="K0W59_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(20167..20460)
                     /locus_tag="K0W59_002628"
     CDS             complement(20167..20460)
                     /locus_tag="K0W59_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBI3023611.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20457..21746)
                     /locus_tag="K0W59_002629"
     CDS             complement(20457..21746)
                     /locus_tag="K0W59_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBI3023612.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21772..22739)
                     /locus_tag="K0W59_002630"
                     /pseudo
     CDS             complement(21772..22739)
                     /locus_tag="K0W59_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
     gene            complement(22759..23523)
                     /locus_tag="K0W59_002631"
     CDS             complement(22759..23523)
                     /locus_tag="K0W59_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HBI3023613.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5857 a         5784 c         6262 g         5848 t
ORIGIN      
        1 atgtgcaatc tatgaggaga cttataatga gttctgataa tgatatcgtt ttgactcata
       61 atctattcgc cttatatata tctggaggtt ttgaggatga tgattaatta ttatcgccag
      121 aacgaaatgt gcacgaaaaa ttgttataag aattgtgtca cttttatcat tgtgatatga
      181 ccacttcgtc attaatctca tgagcgttta aaatactacc gccaaatcac gcaacgaact
      241 ttgctggatt cagtttatgc tgctgtaaat ccgctcgcag taaacctttc agacgcaccg
      301 tgatgttatc agtggttctt taagcgtttt gctggtgtac tcactacaaa cgaattgcga
      361 atcgaatcaa tgtgaaacgg aaaggtacaa tctccctttt tttagtgtta acatcgttca
      421 accgggtgga aggtgagagg ttaatgtctt tcatttgtgt acctgattgc gaaccaattt
      481 agcatttgtt ggctagatgg tttcgcaatg aactgttaat aaacaaattt ttctttgtat
      541 gtgatctttc gtgtgggtca ccactgcaaa taaggatata aaatgcctgt tataactctt
      601 cctgatggca gccaacgcca ttacgaccac gctgtaagcc ccatggatgt tgcgctggac
      661 attggtccag gtctggcgaa agcctgtatc gcagggcgcg ttaatggcga actggttgat
      721 gcttgcgatc tgattgaaaa cgacgcacaa ctgtcgatca ttaccgccaa agacgaagaa
      781 ggtctggaga tcattcgtca ctcctgtgcg cacctgttag ggcatgcgat taaacaactt
      841 tggccgcata ccaaaatggc aatcggcccg gttattgaca acggttttta ttacgacgtt
      901 gatcttgacc gcacgttaac ccaggaagat gtcgaagcac tcgagaagcg gatgcatgag
      961 cttgctgaga aaaactacga cgtcattaag aagaaagtca gctggcacga agcgcgtgaa
     1021 actttcgcca accgtgggga gagctacaaa gtctccattc ttgacgaaaa catcgcccat
     1081 gatgacaagc caggtctgta cttccatgaa gaatatgtcg atatgtgccg cggtccgcac
     1141 gtaccgaaca tgcgtttctg ccatcatttc aaactaatga aaacggcagg ggcttactgg
     1201 cgtggcgaca gcaacaacaa aatgttgcaa cgtatttacg gtacggcgtg ggcagacaaa
     1261 aaagcactta acgcttacct gcagcgcctg gaagaagccg cgaaacgcga ccaccgtaaa
     1321 atcggtaaac agctcgacct gtaccatatg caggaagaag cgccgggtat ggtattctgg
     1381 cacaacgacg gctggaccat cttccgtgaa ctggaagtgt ttgttcgttc taaactgaaa
     1441 gagtaccagt atcaggaagt taaaggtccg ttcatgatgg accgtgtcct gtgggaaaaa
     1501 accggtcact gggacaacta caaagatgca atgttcacca catcttctga gaaccgtgaa
     1561 tactgcatta agccgatgaa ctgcccgggt cacgtacaaa ttttcaacca ggggctgaag
     1621 tcttatcgcg atctgccgct gcgtatggcc gagtttggta gctgccaccg taacgagccg
     1681 tcaggttcgc tgcatggcct gatgcgcgtg cgtggattta cccaggatga cgcgcatatc
     1741 ttctgtactg aagaacaaat tcgcgatgaa gttaacggat gtatccgttt agtctatgat
     1801 atgtacagca cttttggctt cgagaagatc gtcgtcaaac tctccactcg tcctgaaaaa
     1861 cgtattggca gcgacgaaat gtgggatcgt gctgaggcgg acctggcggt tgcgctggaa
     1921 gaaaacaaca tcccgtttga atatcaactg ggtgaaggcg ctttctacgg tccgaaaatt
     1981 gaatttaccc tgtatgactg cctcgatcgt gcatggcagt gcggtacagt acagctggac
     2041 ttctccttgc cgtctcgtct gagcgcctcc tatgtgggcg aagacaacga gcgtaaggta
     2101 ccggtaatga ttcaccgcgc aattctgggg tcgatggagc gtttcatcgg tatcctgacc
     2161 gaagaattcg ctggtttctt cccgacctgg cttgcgccag ttcaggttgt tatcatgaat
     2221 attaccgatt cacagtctga atacgttaac gaattgacgc aaaaactatc aaatgcgggc
     2281 attcgtgtta aagcagactt gagaaatgag aagattggct ttaaaatccg cgagcacact
     2341 ttgcgtcgcg tcccatatat gctggtctgt ggtgataaag aggtggaatc aggcaaagtt
     2401 gccgttcgca cccgccgtgg taaagacctg ggaagcatgg acgtaaatga agtgatcgag
     2461 aagctgcaac aagagattcg cagccgcagt cttaaacaat tggaggaata aggtattaaa
     2521 ggcggaaaac gagttcaaac ggcgcgccct aaccgtatca atggcgaaat tcgcgcccag
     2581 gaagttcgct taacaggtct ggaaggcgag cagcttggta ttgtgagtct gagagaagct
     2641 ctggagaaag cagaagaagc cggagtagac ttagtcgaga tcagccctaa cgccgagccg
     2701 ccggtttgtc gtataatgga ttacggcaaa ttcctctatg aaaagagcaa gtcttctaag
     2761 gaacagaaga aaaagcaaaa agttatccag gttaaggaaa ttaaattccg tcctagtaca
     2821 gatgaaggcg actatcaggt aaaactccgc agcctgattc gctttctcga agagggtgat
     2881 aaagccaaaa tcacgctgcg tttccgcggt cgtgagatgg cgcaccagca aatcggtatg
     2941 gaagtgctta atcgcgtgaa agacgatttg caagaactgg cagtagtcga atccttccca
     3001 acgaagatcg aaggccgcca gatgatcatg gtgctcgctc ctaagaagaa acagtaaggc
     3061 cttcaagtag cactatctgt ggagcctttg ggcttcacag gcattgttcg cctttgtttc
     3121 gtttattaac aatgcgaagt ggaagttatt aaaatgccaa aaattaagac cgtacgcggt
     3181 gctgctaagc gcttcaaaaa aaccggtaaa ggtggtttta agcacaagca cgctaacctg
     3241 cgtcacattc tgactaaaaa agcgaccaaa cgtaaacgtc acctgcgtcc gaaagccatg
     3301 gtttccaaag gcgatctggg cttggtaatc gcgtgcctgc cgtacgcata agccgttaac
     3361 gtttttaact ttttaattag aatatagata caggagagca catatggctc gcgtaaaacg
     3421 tggtgttatt gcacgcgcac gtcacaagaa aattttgaaa caagctaaag gctactacgg
     3481 tgcgcgttct cgcgtatacc gcgttgcctt ccaggctgtt atcaaagctg gtcagtatgc
     3541 ttaccgtgac cgtcgtcaac gtaagcgtca gttccgtcaa ctgtggattg cgcgtatcaa
     3601 cgcagcagca cgtcagaacg gtatttctta cagcaaattc atcaatggcc tgaaaaaagc
     3661 ctctgttgaa atcgaccgta agatcctggc tgatatcgca gtattcgaca aagtagcgtt
     3721 caccgctctg gttgaaaaag cgaaagcagc tctggcataa gccagttgaa agagggagct
     3781 agtctccctc ttttcgtttc aacgccatca aaacattgac ttttatcgcc gtagcctttt
     3841 caataaaggt cttttgaaga gtaccaaaag gtaacgcaag caatgaatgc tgctattttc
     3901 cgcttctttt tttactttag cacctgaatc caggaggcta gcgcgtgaga agagaaacgg
     3961 aaaacagcgc ctgaaagcct cccagtggag gctttttttg tatgcgcgtt ttgaaaaata
     4021 aaaagccagt ctatcaggcg taattgtcgc agccagtctg gacacggcca gtgcggagaa
     4081 accggagcgt acttaagtac gtgaggattt cgagcactgc ccgggaccaa aatggcaagt
     4141 aaaatagcct gatgggatag gctctaagtc caacgaacca gtgtcaccac tgacacaatg
     4201 aggaaaacca tgtcacatct cgcagaactg gttgccagtg cgaaggcggc cattagccag
     4261 gcgtcagatg ttgccgcgtt agataatgtg cgcgtcgaat atttgggtaa aaaagggcac
     4321 ttaacccttc agatgacgac cctgcgtgag ctgccgccag aagagcgtcc ggcagctggt
     4381 gcggttatca acgaagcgaa agagcaggtt cagcaggcgc tgaatgcgcg taaagcggaa
     4441 ctggaaagcg ctgcactgaa tgcgcgtctg gcggcggaaa cgattgatgt ctctctgcca
     4501 ggtcgtcgca ttgaaaacgg cggtctgcat ccggttaccc gtaccatcga ccgtatcgaa
     4561 agtttcttcg gtgagcttgg ctttaccgtg gcaaccgggc cggaaatcga agacgattat
     4621 cataacttcg atgctctgaa cattcctggt caccacccgg cgcgcgctga ccacgacact
     4681 ttctggtttg acgctacccg cctgctgcgt acccagacct ctggcgtaca gatccgcacc
     4741 atgaaagccc agcagccacc gattcgtatc atcgcgcctg gccgtgttta tcgtaacgac
     4801 tacgaccaga ctcacacgcc gatgttccat cagatggaag gtctgattgt tgataccaac
     4861 atcagcttta ccaacctgaa aggcacgctg cacgacttcc tgcgtaactt ctttgaggaa
     4921 gatttgcaga ttcgcttccg tccttcctac ttcccgttta ccgagccttc tgcagaagtt
     4981 gacgtcatgg gtaaaaacgg taaatggctg gaagtgctgg gctgcgggat ggtgcatccg
     5041 aacgtgctgc gtaacgttgg catcgacccg gaagtttact ctggtttcgc cttcgggatg
     5101 gggatggagc gtctgactat gttgcgttac ggcgtcaccg acctgcgttc attcttcgaa
     5161 aacgatctgc gtttcctcaa acagtttaaa taaggcagga atagattatg aaattcagtg
     5221 aactgtggtt acgcgaatgg gtgaacccgg cgattgatag cgatacgctg gcgaatcaaa
     5281 tcactatggc gggcctggaa gttgacggtg tagaaccggt tgccggtagc ttccacggcg
     5341 tggtcgttgg tgaagtggtt gagtgtgcgc agcatccgaa cgctgacaaa ctgcgtgtga
     5401 caaaagtgaa tgtcggcggc gatcgcctgc tggacatcgt ctgcggtgcg ccaaactgcc
     5461 gtcagggcct gcgtgtggcg gtagctacca ttggtgctgt tctgccgggt gatttcaaaa
     5521 ttaaagcggc gaaactgcgt ggcgaaccgt ctgaagggat gctgtgctcc ttttctgagc
     5581 tgggaatttc tgacgatcac agtggcatta tcgaactgcc tgcggatgcg ccgattggca
     5641 ccgacatccg cgaatacctg aaactcgatg ataacacgat cgaaatcagc gtgacaccaa
     5701 accgtgccga ctgcttaggt atcattggtg ttgcgcgtga cgttgccgtg ctgaatcagc
     5761 tgccgctggt tgaaccggaa atcgttccgg ttggtgcgac catcgacgac acgttgccga
     5821 ttacagtcga agcgccggaa gcctgcccgc gctatcttgg ccgtgtggta aaaggcatta
     5881 acgttaaagc gccaactccg ctgtggatga aagaaaaact gcgtcgttgc gggatccgtt
     5941 ctatcgatgc agttgttgac gtcaccaact atgtactgct cgaactgggc cagccgatgc
     6001 acgctttcga taaagatcgc attgaaggcg gcattgtggt gcggatggca aaagagggcg
     6061 aaacgctggt gctgcttgat ggcactgaag cgaagctgaa tgctgacact ctggtcatcg
     6121 ccgaccacaa caaggcgctg gcgatgggcg gcatctttgg tggcgaacac tctggcgtga
     6181 atgacgaaac gcaaaacgtc ttgctggaat gcgctttctt cagcccgctg tctatcaccg
     6241 gtcgtgctcg ttgtcatggc ctgcatactg atgcgtctca ccgttatgag cgtggcgttg
     6301 atccggtact gcagcacaaa gcgatggaac gtgcgacccg tctgctgatc gacatctgcg
     6361 gtggtgaggc tggcccggta attgatatca ccaacgaagc aacgctgccg aagcgtgcaa
     6421 ccatcactct acgtcgtagc aaactggatc gcctgatagg ccatcatatt gctgatgagc
     6481 aggtaactga cattctgcgt cgtctcggct gcgaagcgac cgaaggcaaa gacgaatggc
     6541 aggcagttgc gccgagctgg cgtttcgata tggagattga agaagatctg gtcgaagaag
     6601 tcgcgcgtgt ttacggctac aacaacatcc cggatgagcc ggtacaggca agcctgatta
     6661 tgggtactca ccgtgaagtt gacctgtcgc tcaagcgcgt gaaaacgctg ctcaacgaca
     6721 aaggctatca ggaagtgatc acctacagct tcgttgatcc gaaagtgcag cagatgatcc
     6781 atccaggcgt tgaagcctta ctgctggcaa gcccgatctc tgttgaaatg tcagcaatgc
     6841 gtctttctct gtggaccggc ctgctggcaa ccgtggtgta caaccagaac cgtcagcaga
     6901 accgtgtgcg cattttcgaa agcggtctgc gtttcgtacc agatactcag gcaccgttgg
     6961 gcattcgtca ggatctgatg ttagccggtg tgatttgcgg taaccgttac gaagagcact
     7021 ggaacctggc aaaagagacc gttgatttct atgatttgaa aggcgacctt gaatccgttc
     7081 tcgacctgac cggtaaactg aatgaggttg agttccgtgc agaagcgaat ccggcactgc
     7141 atccggggca atccgcagcg atttatctga aaggtgaacg tattggtttt gttggagttg
     7201 ttcatcctga actggaacgt aaactagatc ttaacggtcg cactctggtg ttcgaactgg
     7261 agtggaacaa gctcgcagac cgcgtggtgc ctcaggcgcg cgagatttct cgcttcccgg
     7321 cgaaccgtcg tgacatcgcg gtggtggtcg cagaaaacgt tcccgcagcg gatattttat
     7381 ccgaatgtaa gaaagttggc gtaaatcagg tagttggcgt aaacttattt gacgtgtacc
     7441 gcggtaaggg tgttgcggag gggtataaga gcctcgccat aagcctgatc ctgcaagata
     7501 ccagccgtac actcgaagaa gaggagattg ccgctaccgt cgccaaatgt gtagaggcat
     7561 taaaagagcg attccaggca tcattgaggg attgaaccta tggcgcttac aaaagctgaa
     7621 atgtcagaat atctgtttga taagcttggg cttagcaagc gggatgccaa agaactggtt
     7681 gaactgtttt tcgaagagat ccgtcgcgct ctggaaaacg gcgaacaggt gaaactctct
     7741 ggttttggta acttcgatct gcgtgataag aatcaacgcc cgggacgtaa cccgaaaacg
     7801 ggcgaggata ttcccattac agcacggcgc gtggtgacct tcagacccgg gcagaagtta
     7861 aaaagccggg tcgaaaacgc ttcgcccaaa gacgagtaat ctgatctaac taaaaaggcc
     7921 gctatgcggc cttttttctt ttcactgtcg aagagtcacc gtaaaatcaa cgccatgaca
     7981 cttcagcaga acggatacca tgctgacact tgcccgccaa caacagcgac aaaatattcg
     8041 ctggttatta tgcctgtcag ttttgatgct gctggcgctt ctcttaagcc tttgcgccgg
     8101 tgaacaatgg atttcgccag gtgactggtt tactcctcgt ggcgaactgt tcgtctggca
     8161 aattcgcctg ccacgtacgc tggctgtatt gctggttggt gcggcgctgg ctatatccgg
     8221 cgctgtaatg caggcgttgt ttgaaaatcc tctggcagaa cctggactac ttggcgtctc
     8281 taacggcgca ggcgtggggc ttatcgccgc ggtattgctt gggcaagggc aactccccaa
     8341 ctgggcgcta gggctgtgtg cgattgctgg cgcgcttatc atcactttaa tactcttacg
     8401 tttcgcccgt cgtcatcttt cgaccagtcg gttattgctg gctggcgttg cattagggat
     8461 tatctgcagc gcactaatga cgtgggctat ctacttttcc acctctgttg atttacgtca
     8521 gctgatgtac tggatgatgg gcggttttgg cggcgtagac tggcggcaaa gctggctgat
     8581 gctggcattg atccccgtgt tgttgtggat ctgttgtcag tctaggccga tgaatatgtt
     8641 agcacttggc gagatctcgg cgcggcaact gggtttaccc ctgtggttct ggcgcaatgt
     8701 gctggtggca gcgaccggct ggatggttgg cgtcagtgtg gcgctggcgg gtgctatcgg
     8761 ctttattggt ctggtgatcc cacatattct tcggttgtgt ggtttaaccg atcatcgcgt
     8821 attacttccc ggctgcgcgc tggcaggggc gagcgcattg ctgctggccg atattgtagc
     8881 gcgcctggca ttagctgccg cagagctgcc tattggcgtg gtcaccgcaa cgttgggtgc
     8941 gccggtgttt atctggttat tgttaaaagc aggacgttag ccgcaaaaag acggtctatg
     9001 attaaaagct aagattttta ctgaccacac ccaggagaaa cgatgcaaga ttccattctg
     9061 acgaccgtag tgaaagatat cgacggtgaa gtgaccacgc tggagaagta cgccggtaat
     9121 gtgctgttaa ttgtcaatgt cgcctcaaag tgtggcttaa cgccgcaata tgagcagttg
     9181 gagaatattc aaaaagcctg ggccgatcga ggttttgtgg tgctgggatt cccgtgcaac
     9241 cagtttctgg aacaagaacc gggcagcgat gaagagatta aaacttactg taccaccaca
     9301 tggggggtga cgttcccgat gttcagtaag attgaagtta atggcgaagg acgccatccg
     9361 ctgtatcaaa aattgattgc cgcagcgccg accgcagtcg cgccggaaga gagcggattc
     9421 tatgcccgta tggtcagcaa aggccgtgca ccgctgtacc aggatgatat tttatggaat
     9481 tttgaaaaat tcctagttgg cagggacgga aaagtcatcc agcgtttttc cccggatatg
     9541 acgccggaag atcccattgt gatggaaagc attaaactgg cgttggcaaa ataatgtcta
     9601 ttgtgatgca gttacaggat gttgcggaat ctacccgcct ggggccgctt tcaggcgagg
     9661 ttcgggctgg ggaaatcctg cacctggtgg ggccgaatgg cgcgggtaag agtaccttac
     9721 tggcgcgaat ggccggaatg accagcggta agggaagcat tcagttcgcg gggcaaccac
     9781 tggaagcatg gtccgcaaca aaactcgcgc tgcatcgcgc ctatctttca caacagcaga
     9841 cgccgccgtt tgcaatgccg gtctggcact acctgacact gcatcagcac gataaaacgc
     9901 gtaccgaact actgaatgat gtcgcagggg cgctggctct tgatgacaaa ctcggtcgta
     9961 gcaccaatca actttccggc ggtgaatggc aacgcgtacg tcttgctgcg gtggtgttgc
    10021 aaatcacacc acaagccaat cccgcaggcc aattgctgct tcttgatgag ccgatgaaca
    10081 gtcttgatgt tgcgcaacaa agtgcgttag acaaaattct gagcgcgctg tgtcagcaag
    10141 gactggcgat tgtgatgagc agtcacgatc tcaaccacac gttgcgtcat gcgcatcggg
    10201 cgtggttgct aaaaggtgga aaaatgctgg ccagtggacg cagagaagag gtgctcacgc
    10261 cgccaaatct ggcgcaggcc tatgggatga attttcgccg tctggatatc gaaggtcaca
    10321 gaatgctgat ttcgaccatc tgatttcatt agtatcccga aaatcgggta attacgacgc
    10381 taaattaatg caagaataaa aaacagagga accgtacgga atgcgtttct gccttatttt
    10441 gatcacagca ctgtttctgg ccgggtgtag ccaccataaa gcaccgccgc caaatgccag
    10501 actttctgat tcgattaccg ttattgccgg tttgaacgac cagctacaaa gctggcatgg
    10561 cacgccgtat cgttatggtg gcatgacgcg gcgcggtgtg gactgttcgg gatttgtggt
    10621 tgtgacgatg cgcgatcgtt tcgatttgca tctgccccgt gaaaccaaac aacaagcctc
    10681 tattggcacg caaattgata aagacgagtt gctgcctggt gacctggtct ttttcaaaac
    10741 gggttccgga caaaatggtt tgcatgtagg tatttacgat accaacaacc aatttatcca
    10801 cgcctctacc agcaagggag tgatgcgttc ctcacttgat aatgtctatt ggcagaaaaa
    10861 tttctggcag gcgagacgaa tctagtgtgt cgaaaaattg acggcgatga tacatcgccg
    10921 tcaatttact cctgaataca gtagtatttt gcagacaaaa attccgtaaa tggattattc
    10981 gtttataatc aggataatct aaaaaaaggc cccctgaaaa agtggttaac atggaacaaa
    11041 tgaaagttac tccttctgta attgttatgt cttatggcct catccctttt agccggatac
    11101 tgaaaaacat ccttcgagag ggacggttac catgaagatt tttttggaga atctttatca
    11161 ttctgattgc tatttcctgc cgataagaga taaccagcaa ctccttgtcg gcgttgagtt
    11221 aattacccat ttctccagtg aagatggcac ggttcgtatc ccgacgagtc gggttatagc
    11281 gcaacttacc gaggagcaac attggcaatt attttcagag cagttggaat tactaaaatc
    11341 atgccaacac ttttttattc aacataaact ctttgcctgg cttaatttaa cgccacaagt
    11401 ggctacatta ttattagaca gggataatta cgcaggcgaa ctattaaaat atccgtttat
    11461 tgagctgttg attaatgaga attacccgca tctcaatgaa ggaaaagata atcgcgatct
    11521 tttgtcctta tcgcaaatgt atccccttgt gttaggcaat ttgggagcag gtaatagcac
    11581 aatgaaagcg gtttttgatg gtcttttcac ccgcgtaatg ctggagaaag gaacaaatca
    11641 cgcatcgctc cttcgaaccg tttattcgtg cgatccaggc gcaaatttcc ccttgttgca
    11701 attgcatcat cgcggggggc attgatacgc cagaaatatt ggcacagatt acccccttcg
    11761 atttccatgc tctccaggga tgcctgtggc ctgccgttcc tataaatcag ataacgacgt
    11821 tggttcagcg ataaccctcc tgtttgctgg tgtttaagac gagagtaacc gtctacacta
    11881 tcaaacagat aggatctatg accctgtctt ttattacccg ctggcgcgat gaattgccag
    11941 aaacctatac agcactttcc cctacgcctt taaataatgc ccggctgatt tggcataata
    12001 ccgaactcgc taacacgctg agtattccat cgtcgctgtt taaaaatgcc gcaggtgtct
    12061 ggggcggcga aaccctactg cctggtatgt caccactggc acaggtttac agtggtcatc
    12121 agttcggcgt ctgggcgggc caactgggtg atgggcgcgg cattttactt ggcgaacaac
    12181 tgcttgctga tggcactaca atggactggc atctgaaagg tgctggcctg acgccttatt
    12241 cgcgaatggg tgatggacgg gcggttttac gttcgacgat acgagaaagt ctcgccagtg
    12301 aggcgatgca ttatctgggc attccgacga cccgcgcgtt aagtatcgtc accagcgatt
    12361 cgccagtgta tcgggaaacg gtggagccag gcgcgatgcc gatgcgtgtg gcaccaagtc
    12421 atctgcgctt tggtcatttc gaacattttt actatcgccg cgagccggaa aaggttcgtc
    12481 agttggctga ctttgccatt cgtcattact ggtcacatct tgaagacgat gaggacaaat
    12541 accgtctctg gtttaacgat gttgtcgcac gtaccgcgtc gttaattgcc caatggcaga
    12601 cggtcggctt tgcacatggg gtgatgaata ccgacaacat gtcgctgctg ggactgacgc
    12661 ttgattacgg gccgtttggt tttcttgatg attacgaacc cggttttatt tgtaatcact
    12721 cggatcatca agggcgttat agctttgata atcaacctgc tgtcgcgttg tggaatttac
    12781 agcgtctggc gcagacattg tcaccatttg ttgccgtaga tgccctgaat gaggccctgg
    12841 acagctatca gcaggttttg ttgacgcatt atggacaacg gatgcggcag aaactgggct
    12901 tcatgacgga gcaaaaagag gataacgcgc tactgaatga attattcagt ctgatggcgc
    12961 gagagcgcag cgattatacc cgcacattcc gcatgctgag tctgaccgag cagcacagcg
    13021 cggcgtcacc gctacgtgat gagtttattg atcgtgcggc atttgatgac tggtttgccc
    13081 gttatcgggg gcgtttgcaa caagacgagg ttagcgatag tgagcgtcag caactgatgc
    13141 aaagcgttaa cccagctctg gtattgcgca actggttggc gcaacgggcg attgaagcgg
    13201 cagaaaaggg tgatatgatg gaattgcacc gcctgcatga ggcgttgcga aatcctttca
    13261 gcgacagaga tgatgactat gtcagtcgtc cacctgactg gggtaaacgg ctggaagtca
    13321 gctgctcgag ttaaaggcta cttagttaac agcagcttgc cagcctgagt tttacggagc
    13381 agatattctt gcccgtcatg atcgataatc agtttgccat ccggacctaa cagtgtctgg
    13441 ctggtaattc gccggatggg ctgcggggaa gcggtcttgt gattggcatc cgtttcaagc
    13501 gtgagttttc tgctatccgt ataacgcata aaaattaata ataattgata taacaatgat
    13561 tcctattatc aatattatgg caggccagag caagcagtaa tgtgttgaga agacaacaag
    13621 ttgcgcagaa atgcccatcc catcaggaat gggcacgcat tcagaagcgg gtatctaccg
    13681 caaaggcgag tttttcgacc aggcgttcgg tatcttccca gcccagacac gggtcggtaa
    13741 tggattgacc gtaagtgagc ggctgaccgc cgacgatttt ttgcgttcct tcgcgcagga
    13801 aactttccgc cataattcca gcaatctccg tagagccatt gcggatttgc tgacaaatat
    13861 cctcacaaac ttctaactgg cgacggtgct gcttctggca gttaccgtgg ctgaaatcca
    13921 ccaccagatg ttcaggtaaa tcaaactcgt gcagcgtatc gcaggctgcg gcgatatcat
    13981 cggcatgata attcggtttt ttgccgccac gcataataat gtggccatac gggttgccgc
    14041 tggtctgata gatggtcatc tgaccatttt tgtctggcga gaggaacata tggctggcgc
    14101 gggctgcgcg gatagcatcc acggcaatcc gcgtgttgcc atcggtacca tttttaaaac
    14161 ctaccggaca ggagagtgct gaagccattt cgcggtggat ctgactttcg gtagtacgag
    14221 cgccaatcgc gccccaactg attaaatcag caataaactg accggtcacc atatcgagga
    14281 actcagtcgc ggtagggaca cccagctcat ttacctgtaa aagtaatttg cgcgccagct
    14341 ccagaccgtg atttacccga tagctgccgt ttaaatccgg atcggagatt agccctttcc
    14401 agccgacaac agttcgtggt ttttcaaaat aggtgcgcat tacgatttcc agccgtgact
    14461 ggtactggtt gcgcagcgac tgcagacggg tggcgtactc cattgcagcg gtgagatcgt
    14521 ggatcgagca ggggccaata atgaccaaca gtcgcttatc ttcaccattc agtatttttt
    14581 caattctgcg gcgggagtcg gtgacatggg tggcgacgcc aggcgttacg ggataccgta
    14641 gcgcgagttc ggcgggcgtt accaggctct caatacgcgc agtacggagt tcgtcagttc
    14701 tgttcattac aagtctcaga aatttgttgc ttctacggca ggactaccgg aaagtgatgc
    14761 gcatcacgat aaaccaatcg cagctgattt caagtgcgag gtgtgtcaca cctcgccggg
    14821 tggttagcat gataacaaaa aaacaaatca atgcactagt tctctagtac attcggcgac
    14881 taaggcccat gatatcgagg atcttggtgg caatctcttc taccgaataa ttggtactgt
    14941 taatccacgg gatctgattt ttacggtaca aggcttccac ttccgcgact tccatcctgc
    15001 actgacgaag cgaggcatag cgactgttct cccgacgttc ctcgcgaatc gccgccagac
    15061 gttccgggtc gatagtcagg ccgaacaatt tatgctgaag cggtttgagc gacgcgggta
    15121 gcaccagatt atccatatcg tcggcaataa aggggtagtt tgccgcgcgg ataccaaatt
    15181 gcattgccag atacagactg gtgggggttt taccacagcg agaaacaccg agcaggatca
    15241 cctgagcctg atccagattg cgcaacgaaa tgccgtcatc gtgggcgagg gtgtaatcaa
    15301 tcgccgcaat gcgcgcatca tatttattga gattattagg gttaaggcca tgggtacgat
    15361 gagcaatcgg cgttggatcc agtttcatct cttgttgtag cggggcaacc agcgcctgaa
    15421 cgatatcctg gcaaaagcct tcactttgca agatgatggc gcgaatctcc ggcaacacga
    15481 tggagtagaa gaccagcggg cgcacgcctg tctggtgata aattgcgtca atctgatcct
    15541 tcactgcccg tgcacggctc tcattttcga caaacggcag cgtgatgctg ctgatagtga
    15601 cgggaaattg tgacattact gcgtgtccta atacctccgc agttattgcc gtaccatcag
    15661 aaatataaaa aacgtggcga tcaacagcat tatccatttt gttcttcccg tgaagcagac
    15721 ataattctat gaaagcataa attaaaagcg cacagaagcg taggacgtaa tgtctggttt
    15781 ataaaatgaa ccttcatttt attttttatg aaaacagcat ttcattttta tggtttcgtt
    15841 tataccgatg gtttatgtgg aaattgtcga atagagcagg tttgcgcaac gctgggatca
    15901 gtcttaaaaa gtaaaaaaat atatttgctt gaacgattca ccgttttttt catccggtaa
    15961 aatatgcaaa gataaatgcg cagaaatgtg tttctcaaac cgttcattta tcacaaaagg
    16021 attgttcgat gtccaacaat ggctcgtcac cgctggtgct ttggtataac caactcggca
    16081 tgaatgatgt agacagggtt gggggcaaaa atgcctccct gggtgaaatg attactaatc
    16141 tttccggaat gggtgtttcc gttccgaatg gtttcgccac aaccgccgac gcgtttaacc
    16201 agtttctgga ccaaagcggc gtaaaccagc gcatttatga actgctggat aaaacggata
    16261 ttgacgatgt tactcagctt gcgaaagcgg gcgcgcaaat ccgccagtgg attatcgaca
    16321 ctcccttcca gcctgagctg gaaaacgcca tacgcgaagc ctatgcacag ctttccgccg
    16381 atgacgaaaa cgcctctttt gcggtacgct cctccgccac cgcagaagac atgccggacg
    16441 cttcctttgc cggtcagcag gaaaccttcc tcaacgttca gggttttgac gccgttctcg
    16501 tggcagtgaa acatgtattt gcttctctgt ttaacgatcg cgccatctct tatcgtgtgc
    16561 accagggtta cgatcaccgt ggcgtggcgc tctccgccgg tgttcagcgg atggtgcgct
    16621 ccgacctcgc atcttctggc gtgatgttct ccattgatac cgaatccggc tttgaccagg
    16681 tggtgtttat cacttccgca tggggccttg gggaaatggt cgtgcagggt gcggttaacc
    16741 cggatgagtt ttacgtgcat aaaccgacac tggcggcgaa tcgcccggct atcgtgcgcc
    16801 gcaccatggg gtcgaaaaaa atccgcatgg tttacgcgcc gacccaggag cacggcaagc
    16861 aggttaaaat cgaagacgta ccgcaggaac agcgtgacat cttctcgctg accaacgaag
    16921 aagtgcagga actggcaaaa caggccgtac aaattgagaa acactacggt cgcccaatgg
    16981 atattgagtg ggcgaaagat ggccacaccg gcaaactgtt cattgtgcag gcgcgtccgg
    17041 aaaccgtgcg ctcacgcggt caggtcatgg agcgttatac gctgcattca cagggtaaga
    17101 ttatcgccga aggccgtgct atcggtcatc gcatcggcgc gggtccggtg aaagtcatcc
    17161 atgacatcag cgaaatgaac cgcatcgaac ctggcgacgt gctggttact gacatgaccg
    17221 acccggactg ggaaccgatc atgaagaaag catctgccat cgtcaccaac cgtggcggtc
    17281 gtacctgtca cgcggcgatc atcgctcgtg aactgggcat tccggcggta gtgggctgtg
    17341 gagatgcaac agaacggatg aaagacggtg agaacgtcac tgtttcttgt gccgaaggtg
    17401 ataccggtta cgtctatgcg gagttgctgg aatttagcgt gaaaagctcc agcgtagaaa
    17461 cgatgccgga tctgccgttg aaagtgatga tgaacgtcgg taacccggac cgtgctttcg
    17521 acttcgcctg cctgccgagc gaaggcgtgg gccttgcgcg tctggaattt atcatcaacc
    17581 gtatgattgg cgtccaccca cgcgcactgc ttgagtttga cgatcaggaa ccgcagttgc
    17641 aaaacgaaat ccgcgagatg atgaaaggtt ttgattctcc gcgtgaattt tacgttggtc
    17701 gtctgactga agggatcgcg acgctgggtg ccgcgtttta tccgaagcgc gtcattgtcc
    17761 gtctctctga ttttaaatcg aacgaatatg ccaacctggt cggtggtgag cgttacgagc
    17821 cagatgaaga gaacccgatg ctcggcttcc gtggcgcggg ccgctatgtt tccgacagct
    17881 tccgcgactg cttcgcactg gagtgtgaag cagtgaaacg tgtgcgcaac gacatggggc
    17941 tgaccaacgt tgagatcatg atcccgttcg tgcgtaccgt agatcaggcg aaagcggtgg
    18001 ttgaagaact ggcgcgtcag gggctgaaac gtggcgagaa cgggctgaaa atcatcatga
    18061 tgtgtgaaat cccgtccaac gccttgctgg ccgagcagtt cctcgaatat ttcgacggct
    18121 tctcaattgg ctcaaacgat atgacgcagc tggcgctcgg tctggaccgt gactccggcg
    18181 tggtgtctga attgttcgat gagcgcaacg atgcggtgaa agcactgctg tcgatggcta
    18241 tccgtgccgc gaagaaacag ggcaaatatg tcgggatttg cggtcagggt ccgtccgacc
    18301 acgaagactt tgccgcatgg ttgatggaag aggggatcga tagcctgtct ctgaacccgg
    18361 acaccgtggt gcaaacctgg ttaagcctgg ctgaactgaa gaaataaaat aaatccccgg
    18421 cggcgttcag tcgccggggt tatgtgatcc ccgaagatga aacttattca atctcttcac
    18481 agacatcctg cgttaatcgc cgtataatat ctttccttaa caaaaacttt tgtattttat
    18541 ctgaggcagt gcgcggtagt ttttcgatta ccacgatatg ttcaggatat ttatattttg
    18601 cgacccgttt acggctaaaa aaagccacca tctcttccag cgataatgaa tgatgcggcg
    18661 ctttctgcac gacataagcg catgatcgtt cacctaaacg ttcatcgggc attgcaacca
    18721 cacaggcatc gtgaatttta ggatgctgca ataaaatatc ttccacttca cggctgctaa
    18781 tattttcgcc gccgcggaca ataatatctt ttttgcgtcc ggtaattttt atatagccag
    18841 cctcatccat acggcagaga tcgccgctgt aataccagcc ttcttcatcc agcgcacggg
    18901 cggttaattc aggttcatca aaatacccca taaacacatt ggggccacgc gatgtttctt
    18961 caccttcgca acctggcggt aaggtcttgc gtgcgtcatc gaccacttta atctctacac
    19021 ctgcggcagc gtaaccatcg gtatgcataa agcgcgacaa agaattatcg agattcacca
    19081 ccgcatgcgg cgaactttct gtggaaccat aaacacttaa taatttaatg ctgcgctgct
    19141 ggcattcgcg cgccactttt ttggggattg tggtaccgcc gcaaagaaag aagcgcagcg
    19201 ctgaaaggtc tgcgggttgt ttctctagta aattcaaaag atcatagaca aacggcgttg
    19261 cgcctagcat acaggtgcaa cgctgctgct caagcagcgc gagacacgca tcaggagtga
    19321 aaatatctaa caacacgctg cgagcgccaa ttaagaatgg tgccgttacg ccatgcagaa
    19381 agcccgttgc gtgaccaagt ggcgcaggca tcataaagac atccagccag gtcagattca
    19441 gccgcgcgca ataagcccgc tcactggcga gaatattgtt atgcgttagc atcacgccct
    19501 ttggcagacc ctcggttccg gaggtaaaca gcaccgcagc taattcatcg ccgtgggtcg
    19561 ttatcgccgt ggtcagtggg atattgtcgg cgataatctg acttaatgag agggaagagg
    19621 tggcgggagc cagtttgtcc acgccgacaa tttgttgtag ttgtggaagc tgattttgca
    19681 gcggcaggat taaatctacc ggacgcgttt gtttaaacaa cgtcggtgca aagaacattt
    19741 ttgcctgaca cttattgagc acccacacca gttctgcttc ccgccaggaa ggcaacagcg
    19801 gcacggaaac cgcaccgatt ttcaggcagg caagatagat aacggtaaat tcacaccagc
    19861 caggcagttg aaatgcgatg cgatcgcctg attcaatacc cttcgctaac atccagtttg
    19921 ccagacagct cgcggcgtga tcgagcgcgc tataggtgta cgatgcacca tgattatcga
    19981 ccacggcaat tttgtctggc atcgcacgag cggtctgctg tcagtaatcg gccagtgaag
    20041 catcgcccca tagcccttgc tgacgatacg ccgcacgacg ttgttcgtta aacgttaatg
    20101 tcactttcat tttggactct cgaaacagaa catcaggccc gagttgcggg cctgtgggat
    20161 gcatgattag ccgtagcgaa aatcaatacc gaaggtgcct gcgggatatt gccactgttc
    20221 gagaatagtg ttaccgcaca gcacccgaca ggtgccgcac tccagacaac cggcgggatc
    20281 aaaatgaatg tttcctacgt catcctgctt ataaagtccg gcagggcagg ctttcattaa
    20341 tttacggaat tcattgatat cgggattttc cgtcagaatg atatgcgggt ggccctcatc
    20401 aacatggaat ttattgacgc ctaatttgat gtcgacgtta accgtagcgt tctggctcat
    20461 agcgcggttg ctcccttaat gccatctttc agcaagttga tcagcccaat tttcttcgcg
    20521 tgtcccatga tcattttgcg taccggctgg tttggtttgc cgtcaatggt gaacatctcg
    20581 ttcatgatgt cggctaccat tcgtgggtat tggctaaaca ggcgcgggtt ttccatcagc
    20641 gccgggatct tgcgaaaatg ctgcatatca cgcatgacgc agctttgttc cagctcacgt
    20701 ttgtattgcg ccagactgtt ggcggagaaa tccgcgcgtt ctttggcggc gatcaccgtt
    20761 gtggcggcag cctgagccga tgcaatggct aaatccatgc cgcggaccgt aaaacccaaa
    20821 ttcaggcaga agcctgcggc gtcaccaacg atcatcacgc cctcgttaac cagttgcggc
    20881 accattgcca gaccgccttc cggcaccata tgcgcggaat attcaagcag tttgccgccg
    20941 ctaatcagcg ggcgaatggc ggggtgttgt ttaaaatctt ccagcatttg cggcacgctt
    21001 ttttgcgcat gggcgatatc acccaatcca caaaccagcc ccaaggatat ggaatccttg
    21061 ttggtataga gaaatccccc gcccatcagg ccgtcagaag gggaaccggc aaacagccag
    21121 gcggcacctt cctcgcccgt aatattaaag cgatcgttga tctgttctgg tgtgaggcca
    21181 ataacctctt taacaccaac agcgtaatga tgcggatcgg aagcgggaac cattcccagc
    21241 gagcggccaa gcattgagtt aacgccatca gccagaatca ccacattcgc ttcgagaata
    21301 tcatccccag cctgcacgcc agtgaccttg tttccttcac gaaccagcgc gtcgacgcga
    21361 acgcctggga taaactgcgc gcccgcctgc tcggcttgtt ccatcaacca cgggtccaga
    21421 cgattacgca atacggtata agatgcgtgt tgcggaacat ctggttgctc gcggtgaaaa
    21481 tcgagggtaa cggcgctttc ttcggttaag aaggagattt tctcgcgtgt gaccttgcgt
    21541 tctaccggcg ctgatactgc aaagcctgga atgattgctt caagtgtgtg ggcataaaga
    21601 cgcccgccgg tcatgttttt acatccggca ctgtcgccgc gttctatcac caggacatcc
    21661 agcccggctc gtgccatgac cagtgcggca acgctaccag caacgcccgc accgaccaca
    21721 atggcatcaa atttgtcatc cgacatgcgt tatgctccct tcccgctgcg tttacagcgg
    21781 gagatgtggt tgaaagggga attggcggct caactggcta atcagggcag ggacgacttt
    21841 gtagatatcg cccaccagac cgtagtcggc atagttgaag attggcgcat ttttatcttt
    21901 attgatggcg acaatcactt ttacgccgtt gccgccaacc atatgttgga tctgcccgga
    21961 gatccccagc gtcaggtaga gatcggattt cagcaacacg ccggagacac cgatataacg
    22021 ttcacgctcc atccagttct cgccttcggc aattggacgt gaacagccga cttcagcatt
    22081 cagcaccgcc gccagttcgt ggaccatttt caggtcatcc tgcgccgcca gaccacgacc
    22141 gacgccaacc acacgttttg ctttgctcag gtccacgctg cttgcggctt tcgcacggcg
    22201 ttcgcgacag agaatttcat gacgcggagc aacataaggt accgtttctg tcgggcactg
    22261 atgagaggta tcactggtgc acggttcctg aacaccgggt gcaagggtaa tgatcgccag
    22321 gggctgttga tcttttcctg agcgaacgct aacccgccat acatccggtg ttcggcgcaa
    22381 atgtgaccat cgacaatatc caccgccgtg gcatcgttca ccagcgccgc attcagttgc
    22441 acacttaacc gtgctgccag agctttacca cgtttcgtcg cggccaacag caacatagcg
    22501 gggtgtttat ctttcagcag ggcagcaata ctttcggcgt aattttcagt gcgttgcagc
    22561 gcgtcgtttt gctcaagaac ataaatacat tttggaccat aaggcataac tgcctgcgcc
    22621 tggtcggtat tttgtacaat ggcatacacc tgttggcccc attgctgagc gccgccaaac
    22681 agttcagcat aacgttcagg attatcgcta aagacccaga cgctgtttaa ttgactcatc
    22741 gtttcttctc cgtaggcttc agttcagggc tttcttcaga tgttccgcca gctcggcaat
    22801 ggcctccggc gaatcgttat cgagaatgat gtgcttacgt tctgtttgcg gcggtacgcg
    22861 aatgccgacc agttcagcaa gtggcgcgct ctggctccag tcaatatcac ttgcctgcca
    22921 ctgatttacc ggttttttac ccgcgccgag aatggctttc atcgaaggaa tgcgtggcac
    22981 gttaatatcg gaggtgacgc agagcacggc tggaacagag agttcaataa cttcaacatc
    23041 atcttcaagc gtgcgttcaa tcaccagtgt attgccctga cgctgaatag cactcactgc
    23101 attaatcacc ggaagttgca gaatttctcc gaccagcaag ccaacctgct gggcataaag
    23161 gtcgccggaa ccttcaccaa agatcagtaa atcgaagccg atcttttcaa ttgctgccgc
    23221 cagcgctttt gcggtatcga gaggcagtgc atgttcaagt tgcgcatcct gcaccaaata
    23281 caggctgtgc ggcccgcggg atagcacgtc tttgcgcact ttcgagttct gcaacaatga
    23341 gccgccaacg gtcagcgcgg ctatctcatc gtcatctgtt gcgagctggc ttgcagcttc
    23401 aatggcattg agatcgaact ggctgatttt ggcgtcggca ttgtcgaaat tcagggtgta
    23461 ttctggagtg acaacaatgt cctgttcttc aggcaccagc ttaaagcagg ttattatttt
    23521 catgatttct cctgtgagtc gcatacggaa ccatctccga tggaaggtaa aaatggcctg
    23581 aaaatagtgt gcgatagcgg tgcggggggt tcgacggagc aagctgtttt ccttgccaac
    23641 aatcttgcaa aaaaaatgcg attgctaaaa caaaagtgca atttgcggca atatagttat
    23701 tcggtaatgc tgccaactta ctgatttagt gtatgatggt gattttaagg t
//
LOCUS       DAETPZ010000059        23308 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.059, whole genome shotgun sequence.
ACCESSION   DAETPZ010000059 DAETPZ010000000
VERSION     DAETPZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.059"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..461)
                     /locus_tag="K0W59_002632"
     CDS             complement(<1..461)
                     /locus_tag="K0W59_002632"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBI3023614.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMV"
     gene            1066..1479
                     /gene="hns"
                     /locus_tag="K0W59_002633"
     CDS             1066..1479
                     /gene="hns"
                     /locus_tag="K0W59_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HBI3023615.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(1623..2531)
                     /gene="galU"
                     /locus_tag="K0W59_002634"
     CDS             complement(1623..2531)
                     /gene="galU"
                     /locus_tag="K0W59_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBI3023616.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(2733..3746)
                     /gene="rssB"
                     /locus_tag="K0W59_002635"
     CDS             complement(2733..3746)
                     /gene="rssB"
                     /locus_tag="K0W59_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HBI3023617.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFHDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(3838..4743)
                     /gene="rssA"
                     /locus_tag="K0W59_002636"
     CDS             complement(3838..4743)
                     /gene="rssA"
                     /locus_tag="K0W59_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HBI3023618.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            4856..5314
                     /locus_tag="K0W59_002637"
     CDS             4856..5314
                     /locus_tag="K0W59_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HBI3023619.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLSVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5364..6206
                     /gene="purU"
                     /locus_tag="K0W59_002638"
     CDS             5364..6206
                     /gene="purU"
                     /locus_tag="K0W59_002638"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HBI3023620.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6366..6450
                     /locus_tag="K0W59_002639"
     tRNA            6366..6450
                     /locus_tag="K0W59_002639"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6400..6402,aa:Tyr,seq:gta)
     gene            6497..6628
                     /locus_tag="K0W59_002640"
     ncRNA           6497..6628
                     /ncRNA_class="other"
                     /locus_tag="K0W59_002640"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(6812..7489)
                     /gene="narI"
                     /locus_tag="K0W59_002641"
     CDS             complement(6812..7489)
                     /gene="narI"
                     /locus_tag="K0W59_002641"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBI3023621.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7489..8199)
                     /gene="narJ"
                     /locus_tag="K0W59_002642"
     CDS             complement(7489..8199)
                     /gene="narJ"
                     /locus_tag="K0W59_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBI3023622.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8196..9734)
                     /gene="narH"
                     /locus_tag="K0W59_002643"
     CDS             complement(8196..9734)
                     /gene="narH"
                     /locus_tag="K0W59_002643"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBI3023623.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(9731..13474)
                     /locus_tag="K0W59_002644"
     CDS             complement(9731..13474)
                     /locus_tag="K0W59_002644"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HBI3023624.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSCSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(13867..15258)
                     /gene="narK"
                     /locus_tag="K0W59_002645"
     CDS             complement(13867..15258)
                     /gene="narK"
                     /locus_tag="K0W59_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HBI3023625.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            15597..17393
                     /gene="narX"
                     /locus_tag="K0W59_002646"
     CDS             15597..17393
                     /gene="narX"
                     /locus_tag="K0W59_002646"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HBI3023626.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17386..18036
                     /gene="narL"
                     /locus_tag="K0W59_002647"
     CDS             17386..18036
                     /gene="narL"
                     /locus_tag="K0W59_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HBI3023627.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18037..19431)
                     /gene="ychO"
                     /locus_tag="K0W59_002648"
     CDS             complement(18037..19431)
                     /gene="ychO"
                     /locus_tag="K0W59_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HBI3023628.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNFGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQMRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            19616..19969
                     /gene="ychN"
                     /locus_tag="K0W59_002649"
     CDS             19616..19969
                     /gene="ychN"
                     /locus_tag="K0W59_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HBI3023629.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20013..20708)
                     /gene="chaC"
                     /locus_tag="K0W59_002650"
     CDS             complement(20013..20708)
                     /gene="chaC"
                     /locus_tag="K0W59_002650"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HBI3023630.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(20866..21096)
                     /gene="chaB"
                     /locus_tag="K0W59_002651"
     CDS             complement(20866..21096)
                     /gene="chaB"
                     /locus_tag="K0W59_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HBI3023631.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSTWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21366..22466
                     /gene="chaA"
                     /locus_tag="K0W59_002652"
     CDS             21366..22466
                     /gene="chaA"
                     /locus_tag="K0W59_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HBI3023632.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGFLSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            22871..22978
                     /locus_tag="K0W59_002653"
     CDS             22871..22978
                     /locus_tag="K0W59_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBI3023633.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
BASE COUNT         5839 a         6082 c         5636 g         5751 t
ORIGIN      
        1 accatgcttg ctttacggcc acaaaaacag atggttttta attcaaccag tttgtcggac
       61 catgccagta agtattggct gccaataaat aattcacctc gaaaatcggt acgtaaacca
      121 taacaaagta cgggtatatc gagttgatcg acaacctccg ataattcata tacttgttgt
      181 ctggttaaaa actggcattc atcaaccagt acgcaatgaa ttgcctgctg ttcatgttcc
      241 gcacgaatct catcaaataa tgatgaattt tggttaaata attttgcagg cgatgacaaa
      301 cctatacgcg aactgacttt cccggcacca aagcgatcat caatttctgc cgtatatacg
      361 acagtgcgca tgccgcgttc ctggtaattg tatgaagatt gcaacaatgc tgtagactta
      421 cccgcattca ttgcggaata gtagaaatat agctgtgcca tcagccacag gccctcaatg
      481 atatgaaata aagttggctg gagtttatca taattcgtca gtttttcagc tcaacagtat
      541 gcacagaagt gggtatatgc gttctccctt acgaagcctt gcataatcct tctgagctat
      601 cattacaact gcctcgcttg ttataagcgg gtaaatgact gctggtaact attcacaatc
      661 tttaacctgt tgcgcaagta atagccctct gttgacctcc aggagatagt gcaatactaa
      721 gtccatgctc ttattgcgac tgttctactt ttcatcattc gcttaatagg gaattctcgt
      781 aaacacaact aatacagaag actgaaaggt cgtcagccta cgataatctc cccataaaat
      841 gtgacatgaa tcaggaagtt ttaacctcac gtgctgcgaa atcatcggtg taaatagggc
      901 tatatgccgc gtcttttctg gctaatttta tgaaaagata tttattggcg gcacaaaata
      961 aagaacaatt ttgaattcct tacattcctg gctattgcac aactgaattt aaggctctat
     1021 tattacctca acaaaccacc ccaatataag tttgagatta ctacaatgag cgaagcactt
     1081 aaaattctga acaacatccg tactcttcgt gcgcaggcaa gagaatgtac acttgaaacg
     1141 ctggaagaaa tgctggaaaa attagaagtt gttgttaacg aacgtcgcga agaagaaagc
     1201 gcggctgctg ctgaagttga agagcgcact cgtaagctgc agcaatatcg cgaaatgctg
     1261 atcgctgacg gtattgaccc gaacgaactg ctgaatagcc ttgctgccgt taaatctggc
     1321 accaaagcta aacgtgctca gcgtccggca aaatatagct acgttgacga aaacggcgaa
     1381 actaaaacct ggactggcca aggccgtact ccagctgtaa tcaaaaaagc aatggatgag
     1441 caaggtaaat ccctcgacga tttcctgatc aagcaataat cttttgtaga ttgcacttgc
     1501 ttaaaatccc gccagcggcg ggatttttta ttgtccggtt taagacaatt taataagaat
     1561 aagctataaa aaaacggcgt cgattgctca acgccgtttc gtggataaca ccgatacgga
     1621 tgttacttct taatgcccat ctcttcttca agccaggctt taaattccgt gccaagggtg
     1681 ttatgacgaa taccgtattc aacgaaggcc tgcatgtaac ctaatttatt accgcagtca
     1741 tggctcttcc ctttcatatg ataggcttcc accgtttctt tttcgatcag catatcaatt
     1801 gcgtcggtga gctgaatttc atcaccagct cccggagggg tttttgccag caacggccaa
     1861 atatccgcgc taagtacgta acgacccaca atagcgagat tagacggcgc aacatccgct
     1921 ttcggttttt ctaccacacc aaccatcggt acgctttcac ccggcgctaa ttcaacgcct
     1981 ttgcaatcca caacgccata tgcggtcaca tcagcaaccg gttcaaccat gatctggcta
     2041 tgacccgttt catcaaagcg gcggatcatc tctgccaggt tatcctgtga caaatcggat
     2101 tcatattcat ccagaataac atcaggcaaa ataacagcta ccggttcatc acccactacc
     2161 gggtgagcac acaataccgc gtgtcccagg cctttcgcca gaccctgacg aacttgcata
     2221 atagtcacgt gcggtggaca aatagactgc acttcatcaa gcagttgacg ttttacacgt
     2281 ttttccagca ttgcctccag ttcaaaactg gtatcaaagt ggttttcaat agagttttta
     2341 gatgagtgtg taaccagcac aatttcagta atgccagccg caatacattc attcacgacg
     2401 tattgaatta atggcttatc gacaagtggc agcatctctt tcgggatggc tttcgtcgcc
     2461 ggcaacatcc tggttcctaa tcccgcaacg gggataacgg cttttttgac tttcgtatta
     2521 atggcagcca tttaaattct cctggactgt tcgtgttttg aacgtgttca tatttctgta
     2581 tcgcatccca gtatatcagc accccgaagg ggctaagttc tgaaaaggag gcgagatagc
     2641 ataattaaga caattactta tacgaatggc gacgatagta ccaccagccg acattagcag
     2701 gtaatgcaaa tttagcccgc gttatcgttt gctcattctg cagacaacat caagcgcagt
     2761 cgaccaccgg ttccccatat ttggcattgc caggcatcgc atcgctggct caattgattc
     2821 aaataagcgt tacctaaagt gcctaacgga acaccattac tgatttgcac ctggtgttcg
     2881 ccggtattta acgtcgcatt cagacccgca gaaaccagaa tgagattttt cagttcgcga
     2941 tgataatagc caactaatag cggaaactgc cccggcagat tggcctgacg aagtaaatgg
     3001 tttacctgct tcaataacgc gcccaactct ggcaaccgtt gattttggtg tgcaagctgt
     3061 tcctgtaata atccgttaaa caatgcgcgt aataacaagg cagcaagtac gccattatgt
     3121 ccagctcggg tgacatcaag gcaataaaat gccagatcgt tttccgaaag tgcggcaata
     3181 tcaagcacca ggccgggttt gtccgcggca accaattgac ggtaattaac ccggcaatgg
     3241 gaaatcacct gctgaaccgg cggttgtagt tcctgtaata atttcgccgc tgcggcaggg
     3301 ttatcaacca ttgcatccca gtcgtgaaaa agcctttcct cttcctcaac gcgcgaatta
     3361 aacatgctgg gatagagaca ggcaaaaacc atctcgcgca agcgattcag atctttaact
     3421 ggtttcagca aaacatcttc aacgcccaga cgtaacgctt tggcaatatc tgccatattt
     3481 tcagtggcag atatcaccag aactggggtc tggtcgcctc tgttacgtat atgctccagc
     3541 agtttaagcc cgttcattcg tggcatcgcg atatcacata tcatcaggtc tggagtgaaa
     3601 cctcccagca actcaagggc atccacccca tcagccgcca gtaccgttgt cgctcccaat
     3661 gaggaaaacc atgaatccag aagcgagcga aatacctgct catcttcaac aatgagaatc
     3721 tgttttccga ccaatggctg cgtcatgttc tctcccctga ccggctttac tcaatagtgg
     3781 catgctattg ccacctgcgc ctgtcagaat taacttaagt gtagataaaa attctggtca
     3841 aatgttggtg cgtaccaacg gcaaaagttc gtccattttc ctttccactg ccagctgtcc
     3901 ggccgcaatg gcagcgtgcg cgcgatggaa atcaagcgta gatatttgcg ggcaaacagg
     3961 ttgaatcaga atatcgggcg gatcacctgc catgcggttc cttttaaggc ggttctccaa
     4021 cacctggata gacgtggtca taatctctgt tgccgttggc gctgtcacag cccgacgtgt
     4081 cgttatgctg cctaaccttt ctttcagacg cgcatgccac ggtagagaat caccattctc
     4141 gctattttct tcactgacat taaaggagag cagatcttgt tgcatcaaat gagcatcgtg
     4201 ctgcaggtcg accgctatca caatatcagc ccccaatgca cgcgtaagag aaataggaat
     4261 tgggttaact actgccccat caaccagcca gtagccgtta tgtgcaacgg gtgccatgag
     4321 tcctggaata ctgcatgatg cgcgaatagc aagatggaga tcgccttcag taaaccataa
     4381 ttcacgcccc gtacttaaat tggtggcaac agccgcaaag cgacgggaac aattttcgat
     4441 ctctgtttcc ggcattattt cgcgatattg attgaagaca cgctcgccgc gcagtaaccc
     4501 accgcgctgc caggagaggt ccatcaggcg taaaacgtcc caataactga aagaggttac
     4561 ccaatcttcc agcgcagata atcgatcgca tgcataggcc gcgcccacca gcgaaccaat
     4621 tgaacatcct gcaacgatat caatttcaat acccactttt tttagcgcat taataacgcc
     4681 aatatgcgac caacctctcg ccgcgccaga tcccagcgcc agccctattt ttatctttct
     4741 cattattccc gcaagattcc cctggaatgc aatcgtagcc acatcgctac tgctcggtta
     4801 acatagtgct actcaggcgg ttaatacgcc gttattgttt ccagggagat tattggtgtc
     4861 tcagctttgt ccctgtggta gtgctgtcga gtatagccta tgttgccacc cttatgtgtc
     4921 tggtgaaaag gttgcacctg atccagaaca tctcatgcgt tcgcgttact gcgcttttgt
     4981 gatgcaagac gctgattatt taataaagac ctggcatccc tcttgtggag cagcggcgtt
     5041 acgtgccgaa ttgatgaccg gatttgcgca tactgaatgg ttgggattga gcgtatttga
     5101 acattgttgg caggacgccg acaatatcgg atttgtcagt tttgttgctc gatttaccga
     5161 aggtggcaaa acaggtgcaa taattgaacg ttcgcgattt ttgaaagaaa acggtcaatg
     5221 gtactatatt gacggtacac gtccgcagtt tggtcgcaac gatccctgcc cttgtggttc
     5281 aggtaaaaaa tttaaaaagt gctgcggcca ataatggttg acggtacggt ttagcaaaca
     5341 ctctcaacaa ggtttttcca gcaatgcatt cactccaacg taaagttctg cgtactattt
     5401 gtccggacca aaaaggtctg atcgcacgta ttaccaatat ttgctacaag cacgagttaa
     5461 atatcgtaca gaacaatgaa tttgttgatc accgtaccgg gcgctttttt atgcgcacgg
     5521 aactggaagg gatttttaat gattccaccc tgctggcgga tctcgatagc gcattgccag
     5581 aaggctccgt gcgtgagctg aatcctgccg gtcgtcgccg gatagtgatt ctggtcacta
     5641 aagaagcgca ttgccttggc gatttgttga tgaaagccaa ttacggcggt ctggatgtcg
     5701 aaatcgcggc agttattggt aaccacgata ctttacgttc tctggttgag cgttttgata
     5761 ttccgtttga gctggtaagc catgaagggt taagccgcaa cgagcacgat caaaagatgg
     5821 cggacgccat tgatgcttat caacctgact atgtggtgct ggcgaagtat atgcgggtat
     5881 taacgccgga atttgtggca cgcttcccga ataaaatcat caatattcac cattcattcc
     5941 tgcctgcgtt tatcggcgca cgtccttatc accaggccta tgaacgtggt gtgaagatta
     6001 ttggcgcaac cgctcactat gtgaatgaca atctggacga aggcccaatc atcatgcagg
     6061 acgttattca tgtcgatcat acctacacag ctgaagatat gatgcgcgca ggtcgtgacg
     6121 tcgagaaaaa cgtcttaagt cgtgcgctat acaaagtact ggcgcaacgc gtctttgttt
     6181 acggtaatcg gacgattatt ctttaatcgc cagcaaaaat aactggttac ctttaatccg
     6241 ttacggatga aaattacgca accagttcat ttttctcaac gtaacacttt acagcggcgc
     6301 gtcatttgat atgatgcgcc ccgcttcccg ataagggagc tggctagtaa aaagcattac
     6361 cccgtggtgg ggttcccgag cggccaaagg gagcagactg taaatctgcc gtcatcgact
     6421 tcgaaggttc gaatccttcc cccaccacca tcactttcaa aagccccgga attctcaaac
     6481 gaatctgcaa tcaaatattc tgcccaagcg gggaaggatg agaagcttcg accaaggttc
     6541 gacttgagcg ccagcgagag agcgttgccg caggcaacga cccgaagggc gaagcgcgca
     6601 gcgctgagta atccttcccc caccaccatc gcttccttga ataatcaaaa cctgttactc
     6661 cgagctaatt ttgcccgatg gcaccacgtt taccgggcct acaatatcct cccctgtagg
     6721 tcggataagc gaagcgcatc cgacaactca ccccgcaccg tgcccataaa aaaaccccgc
     6781 cgaagcaggg tctatgtgaa ctaaaattcg tttagtgacg agcgcgcacc agctggtact
     6841 tacgtgtcag atactccacc ggtacgctcc agatgtgtac cagacgcgag aacgggaaca
     6901 gcaggaataa cgtcatcccc agcaccaggt gcagacggaa gataaacgcc acaccatcga
     6961 ggtgttgaga agcgccaccg tggaaggtca ccaccgactg cgcccagcca accagtttca
     7021 tcatctcgct accgtccata tgctgagcgg agaacggaat ggtcagcagg cccagcgcgc
     7081 actggataac gagcagcgac aggatcagga tatccgctcc ggtagtggtt gcacgcacgc
     7141 gtgggctgaa catacgacgt ttcagcaaaa gcacgccgcc aatcagacac agcacgccgc
     7201 tggcaccacc agcaaacatc gccattttct gtttcacttc aatcggcagc caggcttcat
     7261 acatccagtg cggcgtcaac ataccgaaga agtgaccgac aaaaatcccc agaataccga
     7321 tatggaacag gttcgacgcc aggttcatcc ctttgcgatc cagcatctgg ctggacgccg
     7381 cgcgccaggt gtactgcccg tagtcataac gcagccagct accaatcagg aagaccgccc
     7441 cggctatgta cgggtagata tcaaagaaga acatattcag gaattgcatt agtgctgtcc
     7501 tccggtggtg atattcagat attgcggcgc gacggcaccg gcaaagcgac gctgatgagc
     7561 agtgattgct gaatcaccgc agcctttgtc agcaaagaat ttaacctgct cttcttccca
     7621 gacagcatcc agcgcctgcg gcgtatcatc gcgcgcttcg tcggcaattt tttccgccac
     7681 tttgtcgctg tcgatagcgg tattggccaa tttcagcagc agatcaaaca tcacggcata
     7741 acggctttca cgctgttgca gacgcgcgct cagcaatgcc agaatcggcg cgatatcttt
     7801 caaaccttcc acggcttcgc tttgcggcag ctgcgacagg tactccagat acagcggcag
     7861 atggtccggt aattcgcggc tgtttaactg caagccgtgc tgctcgtact gcgccagcag
     7921 gtccaccatc gcctggccgc ggtcacggga ttcaccgtga acatgttcaa atagcaacag
     7981 tgacgtggcg cggccacggt cgaacagttc gctgtactgg acctgggcat cgagcggatc
     8041 catcgccgtt aaatcgcgca ggaaaatgcc cagcgcatgg gcatcttctt tcgacagatt
     8101 tttcgacgca gcaatcgcct caaacatctc ttgttgatgc tgccataagg cagcatccgg
     8161 atattcaagg agacgcgata caatcacgag ttcgatcatg gatgcggctc cgttttgctg
     8221 gtcacatcga tggcatcgat acgacggctg ttgaacagat tgaatttggt atctgagccg
     8281 tggcagccat caccaaaggt aaagccgcag ccatttttct ccgggaaggc ttcccgtgcc
     8341 agttcacgat gactactcgg caccacaaag cgatcttcgt agttagcaat cgccagataa
     8401 cggtacatct cctgtgcctg ggcttcggtc agaccgacct cttccagcgc acgggtatca
     8461 actttaccgt caacagtttc agcacgtttg taatgacgca tcgccagcat acgtttcagc
     8521 gcacgcagta ccggtttggt atcaccggcg gtcagcagat tcgccagata ctgtaccgga
     8581 atacgcaagc tttcgacgtc tggcagaatt ccgttgctgc ccagctcgcc cgcgtctgct
     8641 gcagactgaa tcggagacag aggcggcacg taccagacca tcggcagtgt gcgatattct
     8701 ggatgcagcg gcagcgccag tttccattcc atcgccattt tataaaccgg cgactgctgt
     8761 gcagcttcaa taacgctcag cggaataccg tctttaatcg cctgctcgat gactttcgga
     8821 tcgttcggat cgaggaacac gtccagctga cgctggtaga gatctttctc gttctcggtg
     8881 ctggctgcac gttcaatcgc gtcggcatcg tacagcagca cgccaagata acggatacga
     8941 ccgacacagg tttctgagca cacggtcggc tgacccgctt caatacgcgg atagcagaag
     9001 atgcacttct cagacttacc gctcttccag ttgaagtaga tttttttgta cggacatccg
     9061 gtgatgcaca tacgccagcc acggcattta tcctggtcga tcaggacgat gccatcttct
     9121 tcacgcttgt aaatcgcacc gctcgggcag gtcgccacac atgccgggtt caggcagtgt
     9181 tcgcacaggc gcggcaaata catcataaag gtgttttcga actggctata catcgccttc
     9241 tggatgttgt cgaagttctt gtctttcgcc agtttatcaa actcaccgcc caggtcatct
     9301 tcccagttcg gccctttttc gattttcgcc atccgttccc cggtaatcag cgaacgcgga
     9361 cgggcaatcg gctgcgattt gctgccttcc ggcgcggtat gcaggttctg ataatcaaaa
     9421 tcgaacggct cgtaataatc gtcgatcccc ggcagatgcg ggttagcaaa gattttaccc
     9481 agcagcatgg ctcggttacc catgcgcggt tgcagtttgc cattgatttt acggatccag
     9541 ccgcctttgt atttttcctg gttttcccag tcagtcggga agccctggcc cggcttggtt
     9601 tccacgttgt tgaaccacgc gtattccacg ccttcacggc tggtccagac gtttttacag
     9661 gtaactgaac aggtgtggca gccgatgcac ttatcgagat tcaacaccat gccgacttgt
     9721 gaacgaattt tcattttacg ctctcctgta cctggtcatt gccttcgcca tctaaccagt
     9781 caatgttctt catcttacgc actacaacaa actcatcgcg gttagaacct acggtgccgt
     9841 agtagttaaa gccgtatgcc agatgggcat agccgccgat catatgcgtc ggtttcggcg
     9901 tgatacgggt gaccgagtta tggataccac cacgctgttg ggtaatttcc gaaccaggca
     9961 ggttaacgat acgttcctgc gcgtggtaca tcatggtcat ccctgccgga acacgctggc
    10021 tgacaaccgc acgggcagtc agtgcaccgt tgctgttgaa gacttcaatc cagtcgttat
    10081 cggcgatacc cagatctttg gcatcggctt cactcaacca gaccaccgga ccaccgcgac
    10141 ctaaagtcag catcagcagg ttgtcgctgt aggtggagtg gataccccac ttctggtgcg
    10201 gcgtcaggaa gttgagcgct ttttccgggt tgccgttgga tttctggcct atcacttctt
    10261 tcaccgaacg ggtgtcgatc ggcggacgat aaaccagcag gctttcaccg aaatcacgca
    10321 tccactggtg atcctgatac agttgctgac gaccagagag cgtacgccat gggatcagct
    10381 cgtgaacgtt ggtgtaaccg gcgttgtaag aaacgtgttc atcttccaga ccagaccagg
    10441 tcgggctgga gataattttg cgcggctgtg cctgaatatc gcggaagcgg atcttctcgt
    10501 cttctttatt cagcgccaga tgcgtatggt cacgaccggt aaattcgctc agggcagccc
    10561 aggctttcac ggctacctga ccgttggttt ccggtgccag cgtcaggatc atctctgccg
    10621 catcaattgc ggtgttcagc atcggctggc cttttgccgg accttccgct ttggtgtagt
    10681 tgagcttacg cagcagatcc atctcgctct gggtgttcca ggcaatccct ttaccgccgt
    10741 taccgatttt ctccatcagc gggccgatag aggtaaagcg ttcgtaagtt gccggataat
    10801 cgcgctctac gaccataatg tgcggtgcag ttttgcctgg gatcaggtcg cattcgcctt
    10861 ttttccagtc tttcacatcc agcggctgcg ccagttcagc ggcagagtcg tgctggatag
    10921 gcagcgtgac gatgtcggtt tctttaccca gatggccgac gcacacttcg gagaatttct
    10981 tcgcgatggc tttgtagatt tcccagtcgc ttttagcttc ccaggccgga tcgaccgccg
    11041 cagacagcgg gtgaataaac ggatgcatat ccgaagtatt catgtcgtct ttttcgtacc
    11101 aggtcgccgt cggcaaaatg atgtcggaat agagacaggt gctcgacaga cggaagtcca
    11161 gcgtaaccac cagatccagc ttgccttcca gaccattgtc ctgccagtcc acttcttccg
    11221 gcttcacgcc gccctgttgc cccagatctt taccctggat accgtgctcc gtccccagca
    11281 ggtacttgag cataaactca tgacctttac cggaagaacc gagcaggtta gaacgccaga
    11341 tgaacaggtt gcgcgggtgg tttttaccgt tttctggttg ttctgccgca aaacggatgg
    11401 aaccctcttt cagggatttc accgtatagt ccaccggatt catcccggct ttttccgctt
    11461 cgccagcgat agtcagcggg ttagtgccta actgcggtgc agacggcagc cagcccatgc
    11521 gttccgcacg gacgttaaag tcgatcaagt gtccggtata gcgggatttg tccgccatcg
    11581 gtgacagcaa ctcttccgcc gtgacggttt cataacgcca ctggctggag tggttataga
    11641 aataagaagt gctgttcatg tgacgcgccg gacgctgcca gtcaagggca aacgccagcg
    11701 gctgccagcc ggtttgcgga cgcagttttt cctgacctac atagtgcgcc cagccgcccc
    11761 cgctctgacc gacacagccg cagaaaatca gcatgttgat cagaccacga tagttcatat
    11821 cgaggtgata ccagtggttc agccccgcac cgacgataat catcgaacga ccgtgcgttt
    11881 tatcagcgtt atcggcaaat tcacgggcga tgcgaataat ctggctgcaa gaaacgccgg
    11941 taatctgctc ggcccaggcc ggggtataag ctttcacatc gtcatagctg gttgcacagt
    12001 taacgtcgtt caggccacgt tccagaccgt agtttgccag cgtcagatca taaacggtgg
    12061 tcaccagggc ggtgctgcca tcagccagtt gcaggcgttt caccggcagt ttgtgcagca
    12121 gcacgttttc cagttccact ttgttgaagt gttccgtgcc gtcgccacca aagtacggga
    12181 agcctacctc agcgatctca tcctggctac ccagcaggct cagttgcagc tcggtttctt
    12241 cgccagtttt gccgtcgcgc tgctcaagat tccatttgcc cttctcgccc cagcggaagc
    12301 caatagaacc gttcggcgca accatttcgc cattggtatt aaaggcgaca gttttccatt
    12361 ccggattgtt ttcctggccc agcgcatcaa ccagatcagc agcgcgcagc atacgacctg
    12421 cagcgtagta accgtcgcgt tcttccagca tcaccagcat cggcatgtcg gtgtagcgac
    12481 gcacatagtc ggtgaaatac tggcttgggt tgtcgaggtg gaattcacgc agcattacgt
    12541 ggcccatcgc cagcgccatt gccgcatcgg tgccctgttt cggtgccagc cacagatcgc
    12601 acagtttggc gatttcagcg tagtctggtg tgacggcaac agttttggtg cctttgtaac
    12661 gcacttcagt aaagaagtga gcatccgggg tacgcgtctg cggcacgttt gaaccccagg
    12721 cgatgatgta gctggagtta taccagtcag cagattccgg tacgtcagtt tgctcgcccc
    12781 aggtttgcgg agacgcagga ggcaggtcgc agtaccagtc gtagaagctt aagcaagtac
    12841 cgccaatcag cgagagatag cgtgcacccg atgcgtaaga aaccatcgac attgccggaa
    12901 ttggcgagaa accagcaaca cggtccgggc cgtagttttt gatggtgtaa acgttagatg
    12961 cggcgatcag ttcgttcacc tcctgccaag aagaacgaac aaatccaccg cgtccacgcg
    13021 cctgcttaaa gcttttcgct ttatcggcgt cttcaatgat agaagcccat gcctcaaccg
    13081 gatcgctatg cagcgccttc gcttcacgcc acattttcat caggcgtttg cgcatcatcg
    13141 ggtatttcag gcggttggca ctgtaaagat accaggagta gctggcaccg cgcgggcagc
    13201 cgcgaggttc atggtttggc agatccggac gggtacgcgg atagtcagtc tgctgggttt
    13261 cccaggtgac cagaccgttt ttgacgtaga ttttccagct gcaggagccg gtgcagttta
    13321 ccccgtgggt agagcggacg attttgtcat gctgccaacg ctggcgatat ccatcctccc
    13381 agtcacggtt ggtattgaga agctggccat gcccatcggc aaaggtttca cccttctgtt
    13441 tgaagtagcg aaaccggtcc aggaatttac tcatcggttt tctcctgtgg gagcctgtcg
    13501 gccctctgat aaatcgacat tgctaatgtt taagggcaag gtaacgctct gaaacagcgt
    13561 gggaattgat aacgatcaag agtgatgggg atgaaaaata aagtaaattc ctaagatacc
    13621 cctttaggat aattaacagt tacagcataa catgctgtaa attaagacgt tttatacctg
    13681 tagttattta aactattaag atttttatgc aaatgggtat taaggagtat tccccatggg
    13741 taacatatta atggctctta atgaaatccg gcgaaataag ccatataaca ctaatatgtt
    13801 gtaactaaat gacgcataaa aaaagcgcgg catcatgccg cgcaaaggat aatcaaatat
    13861 taagatttat tttttagaat gccgaccata taccgcccaa gtaatcacta cgcaggcgat
    13921 atagaaaatc aaaaatactt tcattgcacc tactggcgaa cccgttaatg ccagcgagct
    13981 accaaacgct ttcgggataa agaagccacc aatcgcgcca atcgcagaga tgaaacccag
    14041 cgccgctgcc gtgccggttg ccgcttcacg catcgcacgt tcgtcagaac ccccttctgc
    14101 tttcacgcga tccattgtca gtttacggaa gatcactgaa atcatctgga aagtggaacc
    14161 actacccagc ccagctgtca ggaacagcgc caggaagacc gcgaagaacg ccatgaagct
    14221 tccgccctgc ccgtcagtcg gtaaggtcag gaacagcagg ccgctgaaaa tcgccatcag
    14281 aataaagttc accagcgtga cacgagttcc gcccagacgg tcagataatg caccacctgc
    14341 agaacgcgcc agcgcaccaa taaacggccc gaagaaagcg tattgcagaa tctgaacatc
    14401 cgggaactgc gtttttgaca gcatagcaaa gcccgcggag aagccgatga aggagccgaa
    14461 ggttgccaga tacagcaggc tcataatcca cagatgaccc cgtttgagta ccggcaactg
    14521 ctccttgatg gaggctttcg aggtagcaag atcgttcatg ccaaaccacg ccgcaatggt
    14581 gaagatggca aggaacggca cccatatcca ggacgcattc gccagataca gctcagtccc
    14641 atccggctgt ttgacgccct ggctaccaaa tactgcgaaa atcgacagtg ataccaccag
    14701 cggagcaacc aactgcatga cgctgacgcc catgtttccc agaccaccat tcagacccag
    14761 cgcgccaccc tgcttctgtt tcggaaagaa gaagctgatg tttgccatac tggatgcgaa
    14821 gttcgcgcca gcaaaaccgc atagcagaga gatgatgatg aagacgctat aaggcgtgga
    14881 ggtatcctgc acggcaaaac ccagccagac gcaaggaata atcagaatac cggtgctgaa
    14941 cgccgtccag cgacgaccac cgaagatagg aaccataaag gagtatggaa cacgtaataa
    15001 cgcgccagaa accgaaggca gcgcagtcaa cataaatagc tgatcggtcg taaaattaaa
    15061 accgactttc ggtaagttca ccgcaacagc gctgaacaac atccatacgc aaaacgccag
    15121 cagcagacag ggaacggaaa tccacaggtt gcggctggca atacgttgac cgcgttgttg
    15181 ccagaacgca ggatcttccg gtcgccaatc tgttatgaca gctccagtag ccctttcgga
    15241 ggcagatgag tgactcatag acacctctga tactcgtttc gatgtctgcc accttagtgt
    15301 ctggagctaa aggcaatttg atgtaaatca aacgataaga taactttatc tatcattgat
    15361 atttatcatt acccatagtg agtacagtga tttcataaaa attatgagat ttttcacggt
    15421 gctgtagaat ccctaccctt accgatgtaa agcgactaac cacacggcaa ataaggagta
    15481 actctttccg gctatgggta tacttcagcc aatagccgag aatactgcca ttccagaata
    15541 tatcgtcaca ttcattaagg ttattgctca tttaaagcct gaaggaaggg gtttacatgc
    15601 ttaaacgttg tctctctccg ctcaccctgg ttaatcaggt tgcgcttatt gtgttgcttt
    15661 ctactgctat tggactggca gggatggcgg tttctggctg gctggtgcaa ggcgttcagg
    15721 gcagcgccca tgcgatcaac aaagcgggat cgctgcgcat gcaaagttac cgtctgttgg
    15781 cggcagtgcc attaagcgag aaagacaagc ccttaattaa agagatggaa caaacggcat
    15841 ttagcgccga gttgactcga gcagcagaac gagacggaca actggcgcaa ttacagggtt
    15901 tacaagatta ctggcgtaat gaactgatcc ctgcgctgat gcgtgcacaa aaccgagaaa
    15961 cggtgtcagc ggatgtcagc cagtttgttg ccgggcttga tcagctggta tctggttttg
    16021 accgcaccac ggaaatgcgt attgagacag tggtactggt ccatcgggta atggcggtat
    16081 ttatggcact tttactggtg ttcactatta tctggttgcg ggcgcgactg ctacaaccgt
    16141 ggcggcaact gctggcaatg gcgagtgccg tcagccatcg cgattttacc caacgcgcaa
    16201 acatcagcgg gcgcaacgaa atggcgatgc ttggaactgc gttgaacaat atgtctgcag
    16261 aactggccga aagttatgcc gtacttgagc agcgggttca ggagaaaacc gccgggctgg
    16321 agcataaaaa tcagatcctc tcttttttat ggcaggctaa ccgccgtttg cattcccgcg
    16381 ccccgctgtg tgaacgcctg tcaccggtac tcaacggctt acagaattta accctgctac
    16441 gtgatatcga actgcgggtg tatgacactg atgatgaaga gaatcatcag gagtttacct
    16501 gccagccaga tatgacttgt gatgataaag gctgccagct ctgcccgcgc ggcgtattac
    16561 ccgttggtga tcgcggcacg accctgaagt ggcggctggc tgactctcat acgcagtacg
    16621 gtattttgct ggcgaccctg ccgcaggggc gtcatcttag ccatgatcaa caacaactgg
    16681 tggataccct ggttgaacaa cttaccgcca cgctggcgct ggatcgccat caggaacgtc
    16741 agcaacagtt gatcgtgatg gaagagcgtg ccaccattgc gcgcgaactg catgattcta
    16801 ttgcccaatc tctctcttgc atgaagatgc aggtgagttg tttacagatg cagggcgatg
    16861 cgctgccaga aagcagccgc gaactgttaa gtcagatccg taacgaactg aatgcatcct
    16921 gggcgcagtt gcgtgaattg ctcaccacat tccgcttgca gctcaccgag cctggattac
    16981 gtccggcgct ggaagcgagt tgcgaagagt acagcgccaa atttggcttc ccggtgaagc
    17041 tggattatca attgccgcct cgtctggtgc cttcacatca ggcaatccac ttgttgcaaa
    17101 ttgcccgtga ggcattaagt aacgccctca aacattcgca agcgagtgag gtcgtggtga
    17161 cggtggcgca aaacgataat caggtcaaac tgaccgtcca ggataacggc tgcggcgtgc
    17221 ctgaaaatgc catccgcagc aatcactacg gcatgataat aatgcgcgac cgtgcgcaaa
    17281 gtttacgagg cgattgccgc gtccgccgtc gtgaatcagg tggcaccgaa gtggtggtca
    17341 cctttattcc cgaaaaaact ttcacagacg tccaaggaga tacccatgag taatcaggaa
    17401 ccggctacta tcctgctgat tgacgatcac ccgatgctgc gaactggcgt aaaacagctt
    17461 atcagtatgg caccagatat caccgtggtt ggcgaagcga gtaatggcga acagggtatt
    17521 gaactggcgg aatctcttga tcccgatctg atcctgttag atctcaatat gcccggcatg
    17581 aacggtctgg aaacgctgga taaactgcgc gaaaagtccc tctcagggcg cattgtggta
    17641 ttcagcgtct ctaaccatga agaagatgtg gtcaccgcac tgaaacgcgg cgcggatggc
    17701 tatctgttaa aagatatgga accggaagat ctgctgaaag cattgcatca ggcagctgct
    17761 ggcgaaatgg tattaagcga agcattaacg cctgttctgg ccgccagctt gcgcgctaac
    17821 cgtgccacta ctgagcgcga tgttaaccag ttaaccccac gcgagcgcga tattctcaag
    17881 ctgattgccc agggtttgcc gaacaagatg attgcccgcc gcctggatat caccgaaagc
    17941 acagtaaaag tgcacgtcaa gcacatgctg aagaaaatga agctcaagtc tcgcgtggaa
    18001 gcagcggtat gggtgcatca ggagcgcatt ttctgattac ggttcccagc gcagttcgtc
    18061 gtttgacaat gcatcgaacg gttcgacaag cgttagcgtg atctcattgg aggagacacg
    18121 ctgcccctgg ttatcttcca ccaccacaga caatcgccag tgattgcttg ccccttcccc
    18181 gttctgccag tcaggcatga tcagcgtcca gccctccgcg ctgttggctt gtgcgcccgg
    18241 cgtcaaactt aatatctgcg tatcgccctg ccaaatcagt tgccgaatac cgtaacgact
    18301 gcgcatttgt aatttcagcg gcactgtttc gccaggtttt agatcccacg gcggtgtcgc
    18361 cagaaacacc gttaacgttt ttcgctgtcg gtactcaaga gtcggtagat tatttcgctg
    18421 cggattgtca tagcgactac cacgtaacga ctgactttcg gcaacctcgc ccgcagaaag
    18481 ttgttttttg agcggtacac caaagcggta attaagattc agcccgaggt tattttgatt
    18541 ttcgccactt tccccctgtt tatgctgggc cgtcacagtg actaatggca caggggtgta
    18601 atttaatccc agactcaacg cgacgggatt gtgataaccc gtaccaaagt taaacaaatc
    18661 aacacgatca ccaaaatact gttctaggct gacactggta ttgaggtgtt gatagaacgg
    18721 catgcgcatc cgagctgtca ggtcgtaccc gcgcgccatc cgttgttcct gcgtggctgt
    18781 ctgttcatgc catgcagcaa acggctgata aaagtttgcc gataatcgca aatattcgcc
    18841 ccacgcttcg gcaccaaagc ccgctcgctg aagattttcg tcctgcaagt tgtcataaaa
    18901 agtgttataa cccaccagcc agttgccgcg cgcccagcgt tgcccaacgc ccacattgct
    18961 caccaaccca ttatcctgct gagtaagacc aagctggctc caggtgagat aacgatcatt
    19021 atcttgtaac ggcacaaacc agcttccacg actgccggtg aaatgtcctt cgttatccac
    19081 tttgacgtca acactggcat ttccccacgg tgatagccag gactctacgt gctgattaac
    19141 ctgttgacta agcgcgtcgc ggacttttcc caatgcgaaa gcttttgcct gctcgccagt
    19201 atccagcccg ttatcattca tactggtttc gccgaaatct ttcacaattt cagcaaagtg
    19261 tttttcccca tcatgatttt cgggagccat gcctaaatcc ggcagaccat cattgttatt
    19321 atcaaaggga tttgccgctt tttgctcgaa ggtagattgt gcgttagctg taccgcctgc
    19381 tacaagcaag agtaaataaa acgggataat gcgaggaacg aaacggctca aaacgtcggt
    19441 aacttccggt aataatgaaa aaccatagcc ttaaagaata acgattactg gcaacttttg
    19501 tcgtattttc tcgcgctttc aggaaaatcc ttaaaaatgt gaccgattgg tcagacgctg
    19561 cagaagcagt aagatagaag atgagtgtct aattccctga atacaggagt aaataatgca
    19621 aaaaatcgtg atcgttgcca atggcgcacc ttacgggagc gaatccttgt ttaacagctt
    19681 gcggctggcc attgcgttac gagagcagga gagcaatctg gatctgcgtc tgttcctgat
    19741 gtctgatgcg gtcacagccg ggttgcgcgg gcaaaaacca ggggaaggct acaacattca
    19801 gcaaatgctg gagatcctta ccgctcagaa tgtaccggtg aaattgtgca aaacctgtac
    19861 cgacgggcgc gggattagca cacttcctct gattgatggg gtggaaatcg gtactcttgt
    19921 ggaactggcg caatggacgc tgtcagccga taaagtgctc acattttaat gtttaagggg
    19981 gcgtctgacg ccccactatg agggaagttt actcaggcga atcccggcct taacacacca
    20041 tccggataat tctccgccag tagttttttt accgatacca gcaaatcatt cagcccatcg
    20101 tcctgcattc ccagtttgat aagctcctgt tccagtgaaa acaggtattg tgcattggtt
    20161 cccagcggac cgctcgccgc cgcaatcaac ggcgcgatga cctgagcgcg agtgtcagat
    20221 tcatattctg gatgtcgcgg gtccataata aacacaatgg cgtttactgt gcgtccatca
    20281 tcaagatcaa gctgacacca ggttggcaga taacagccgg taatcatctc gcgcttccac
    20341 aacagggtta gttcctgctc cagcgtctct tctggtagtc gataggcgac gcctgtggtg
    20401 cgtccgccct ctttcagtgc aagcatccgt cccggctggt gcgcagtccc gcgcccggcg
    20461 gtcaggcgca ggcaaaacgc gcgatgccat ccaaccagtg taccggtgca tgattcggta
    20521 aactccagtg ccggattcca catcaacgat ccataaccga agatccacac cggtccctca
    20581 tcaggtcgac aagccagcgt cgccgccagc gaagccgccc gttgttctgc tgaccataag
    20641 agtgattctt caatggcacc aaatgccgtt ttacaatcgg cattcatcaa gaaatcacgc
    20701 gttatcacct tacacctccg ccactgcata cttccctgcg acaacttttt ggtgtcttcc
    20761 agacattttt accgctcatt ttgaaaccac taacgggaca atatgcaatc cacggaccac
    20821 ttgcaaggca acaccgcacg aatagcttta actaagctac cggttttacg attttttatg
    20881 ccatttatca tcatcccctt tggcatattc atgcttcaca gcagcccagg ccactttatg
    20941 cgcggtttct tcgcgactgg cgtcatcacg ccgatcttct ttatctttat attgatccca
    21001 tgtgctgttg aacgcttctt tatagatatc ctgggcatga gacggtagaa cgtgctttac
    21061 gctttccggc agatcgcttt tcgttttata cggcatcgtt gactcctttt acaaagttga
    21121 acattaagta tggtcaacaa tatctactct tgccaaaaca taagaatttt catgtcagac
    21181 tgttcttaag aaacgctaaa acaaacattg tctaaacgta atacttattt ttatgcccca
    21241 atgagtaaaa aatcgacagc ggggtaaaaa ttcgtaaaat gttaccgata tccagacact
    21301 ttctgttatt tttcctacct attttttaca tttccgataa attcatctgc aaagggataa
    21361 ccataatgtc aaatgctcaa gaggcggtaa aaacccgcca caaggagact tcgcttattt
    21421 tcccggttct ggcgctggta gtgctgttcc tgtggggaag tagccagaca ctaccagtgg
    21481 tcattgccat caatcttctt gcgcttattg gttttttaag tagcgccttt agtgttgtcc
    21541 gtcatgcgga cgtattagcc catcgcctgg gagaacctta cggttcgctt attcttagcc
    21601 tttcagtggt tattcttgaa gtcagtttga tttcagcttt aatggcaacc ggcgacgccg
    21661 cgccaacgct aatgcgtgat acgctctatt caatcattat gattgttacc ggtgggctgg
    21721 ttggcttttc attattgttg ggcggtcgta agtttgccac ccaatatatg aatctgtttg
    21781 gtatcaagca gtatttaatt gcgctgttcc ccctggcgat aatcgtactg gtatttccaa
    21841 tggctctgcc tgcggcgaat ttttcaaccg gtcaggcgtt actggtagca ttaatttctg
    21901 cggcaatgta tggcgtattt ttgctgatcc aggccaaaac gcatcaaagt ttgtttgtct
    21961 acgagcacga agatgacagt gatgacgacg acccgcacca cggtaaaccg tctgcccata
    22021 gcagcctgtg gcatgctatc tggttgatta tccatctgat tgccgttatt gcagtcacca
    22081 aaatgaacgc cagctcgctg gagacattgc tcgacagcat gaatgcccct gttgccttta
    22141 ctggcttcct ggtggcactg ttgattctgt cgccggaagg tttaggtgcg ttaaaagcag
    22201 tgttgaacaa ccaggttcag cgcgcgatga atctgttctt tggttcagtg ttagcaacaa
    22261 tttcgcttac cgtacctgtc gtcacgctaa ttgcctttat gacgggtaac gaattgcagt
    22321 ttgcacttgg tgcgccagaa atggtggtga tggtggcctc tttagtgctg tgccatatct
    22381 ccttctccac cggacgtact aacgtactca atggcgcagc gcatctggca ctgtttgccg
    22441 cctatttgat gacgatattt gcctgacagt tatatctcct ccgatgacgg aaatcgggag
    22501 agataagata aattcctgct tattgtattt cttcatacta cctggacagc tcgttaatca
    22561 gaattgcgag cctgtaccac aacctgcacg aaaccactgc aacagctgtt gaaaagccgg
    22621 aatgtactct ttattgcaaa catttcaggg atgaagtgaa gatgaattga gacgtagtat
    22681 ccacaccaag ccggaattat ccggtgaggc gcaatgttgc gggggcttta tccctggtgg
    22741 cattggttgc tggaaagaga aaacccccgc acgttgagag gtaaaacctg acaacaaaac
    22801 gggggctaat cttgaaacca gacacttcaa gaatagccgc aaaatgttgt cattacaaca
    22861 aggcggctat atgacgctcg cgcagtttgc catgactttc tggcacgacc tggcagcacc
    22921 gatcctggcg gggattatta ccgcagcgat tgtcagctgg tggcgtaacc ggaagtaagg
    22981 tgaaaacggg cgtcaggctg ccgtgatggc aatatgcgcc cgaaaccggc cgcaggggta
    23041 acagcctgcg gccatttttg tctttatctc ttccacaagt aaatattggc aacccgagct
    23101 ggaagcatca tccagagcac tcatgaaacg actgtaacag ctgttgaaaa accggaatgc
    23161 actcttcatt gcaaacattt cagggatgaa gtgaagatga attgagtcgt agtatccaca
    23221 ccaagccggc gttatccggt gaggctcaat gttgcggggg ctttatccct ggtggcattg
    23281 gttgctggaa agagaaaacc cccgcacg
//
LOCUS       DAETPZ010000060        22496 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.060, whole genome shotgun sequence.
ACCESSION   DAETPZ010000060 DAETPZ010000000
VERSION     DAETPZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.060"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..157
                     /locus_tag="K0W59_002654"
     CDS             <1..157
                     /locus_tag="K0W59_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3023634.1"
                     /translation="ISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHY
                     LNIKHYQ"
     gene            complement(196..867)
                     /gene="queE"
                     /locus_tag="K0W59_002655"
     CDS             complement(196..867)
                     /gene="queE"
                     /locus_tag="K0W59_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HBI3023635.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            1006..1146
                     /locus_tag="K0W59_002656"
     CDS             1006..1146
                     /locus_tag="K0W59_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023636.1"
                     /translation="MSEENKENGFNHVKIFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            1160..2032
                     /locus_tag="K0W59_002657"
     CDS             1160..2032
                     /locus_tag="K0W59_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HBI3023637.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTGLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQY
                     PANPSESESTSSTDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDL
                     FLFSDKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(2092..3390)
                     /gene="eno"
                     /locus_tag="K0W59_002658"
     CDS             complement(2092..3390)
                     /gene="eno"
                     /locus_tag="K0W59_002658"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBI3023638.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(3478..5115)
                     /gene="pyrG"
                     /locus_tag="K0W59_002659"
     CDS             complement(3478..5115)
                     /gene="pyrG"
                     /locus_tag="K0W59_002659"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HBI3023639.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVVGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(5343..6134)
                     /gene="mazG"
                     /locus_tag="K0W59_002660"
     CDS             complement(5343..6134)
                     /gene="mazG"
                     /locus_tag="K0W59_002660"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBI3023640.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(6230..8464)
                     /gene="relA"
                     /locus_tag="K0W59_002661"
     CDS             complement(6230..8464)
                     /gene="relA"
                     /locus_tag="K0W59_002661"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HBI3023641.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LYGS"
     gene            complement(8512..9813)
                     /gene="rlmD"
                     /locus_tag="K0W59_002662"
     CDS             complement(8512..9813)
                     /gene="rlmD"
                     /locus_tag="K0W59_002662"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBI3023642.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9870..12626
                     /gene="barA"
                     /locus_tag="K0W59_002663"
     CDS             9870..12626
                     /gene="barA"
                     /locus_tag="K0W59_002663"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HBI3023643.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVITLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12858..14198)
                     /gene="gudD"
                     /locus_tag="K0W59_002664"
     CDS             complement(12858..14198)
                     /gene="gudD"
                     /locus_tag="K0W59_002664"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBI3023644.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(14219..15559)
                     /locus_tag="K0W59_002665"
     CDS             complement(14219..15559)
                     /locus_tag="K0W59_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBI3023645.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFETFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15561..16913)
                     /gene="gudP"
                     /locus_tag="K0W59_002666"
     CDS             complement(15561..16913)
                     /gene="gudP"
                     /locus_tag="K0W59_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HBI3023646.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGQFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(17347..17796)
                     /locus_tag="K0W59_002667"
     CDS             complement(17347..17796)
                     /locus_tag="K0W59_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBI3023647.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17814..18596)
                     /gene="truC"
                     /locus_tag="K0W59_002668"
     CDS             complement(17814..18596)
                     /gene="truC"
                     /locus_tag="K0W59_002668"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HBI3023648.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18596..18925)
                     /locus_tag="K0W59_002669"
     CDS             complement(18596..18925)
                     /locus_tag="K0W59_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HBI3023649.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19547..20092)
                     /gene="syd"
                     /locus_tag="K0W59_002670"
     CDS             complement(19547..20092)
                     /gene="syd"
                     /locus_tag="K0W59_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HBI3023650.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            20160..21008
                     /gene="queF"
                     /locus_tag="K0W59_002671"
     CDS             20160..21008
                     /gene="queF"
                     /locus_tag="K0W59_002671"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HBI3023651.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            21120..22484
                     /gene="ppnN"
                     /locus_tag="K0W59_002672"
                     /gene_synonym="ygdH"
     CDS             21120..22484
                     /gene="ppnN"
                     /locus_tag="K0W59_002672"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HBI3023652.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5565 a         5956 c         5335 g         5640 t
ORIGIN      
        1 tatcagcaag cgttacactc agcgcattga gcgacataat ctgaatctga gacaacatct
       61 ggcaaggctg ggacggaagt cactgtcgtt ctcaaaatcg gtggagctgc atgacaaggt
      121 catcgggcat tatctgaaca taaaacacta tcagtaagtt ggagtcatta ccttataacg
      181 cttataaatg tgtaatcagg caatatttaa atatttatgt gtttgcatcg acaaacgcca
      241 attacgcgca atacaggttt caatgcacaa tcgtgtggca tcatcctttt ggctaatcgg
      301 ctgcagtgca atgactcgcg gtttatcatc ggtcagcgtt gccagtagtt catccagtgc
      361 ttcaatatcg cgtacgcgcc ccactggatg cttgatttcg ttggctcgct ccagtgcctg
      421 tgacaacact tcatagccgc cgcgcatgtt cagctttggc gatacggtaa cccaggtatt
      481 cggtgtgcag cgtacttcat gagttccact ggtttcgatc tggcagctaa aaccgttctt
      541 ttcgagcagg tcagtcagtg gcagcaaatc atgaatgcaa ggctcaccac ccgtaatcac
      601 cacatgccgc gcggtatatc cctgacgacc aatgacagcc agcaagtctt cactgctcgc
      661 cgccccccac ttatcactct ctttggtctt cgccagaatg ctgaaaaggg agacttcccg
      721 atcctcaagc ttttcccagg tgtgtttggt gtcgcaccag gcacagccaa ccgggcatcc
      781 ctgtaaacga ataaaaatgg cggggacgcc ggtaaagtaa ccctcacctt gcagggtctg
      841 gaacatctcg ttaatcgggt actgcatagc attctctgtg aagtggataa ttgttaatta
      901 ttgcagatcc tgccgcaaca atcatgtctt attaacattc tgttacatgc agatttaaag
      961 aaaaatacga aacatatcgt taataattaa agggagtaac gtattatgtc agaagaaaat
     1021 aaagaaaatg gatttaatca tgtcaaaata ttcaccaaaa ttatatttat tttttctgta
     1081 ttagttttta atgataacga atctaaaatt accgatgccg ccgtcaattt atttatccag
     1141 atttaatgag aaatttataa tgcgctattt catactaatg ttcactttcg tatgttcctt
     1201 tgttgcagcc caaccaacta ttgtcccaca attacagcaa caagttacgg gtcttacgag
     1261 tagcttaaac tcacaagaaa agaaagaact gacgcacaag ttagaatcta ttttcaataa
     1321 tacccaagtg caaattgctg tattaattgt ttccacaacc aaagatgaga caattgaaca
     1381 atatgctaca agagtttttg acaattggcg tttaggagat gccaaacgta atgatgggat
     1441 actgatcgtt gttgcctggt cggatcgcac tgtccgcatc caggtaggtt atgggctgga
     1501 agaaaaggta accgatgctc tggctggaga tatcatccgt agcaacatga tacccgcatt
     1561 taaacaacaa aaattagcta agggattaga gttagctata atcgctttga ataaccaact
     1621 cacttcacaa caccaatatc cggctaatcc ttcagaaagt gaatcaacgt cttccactga
     1681 tcattattac tttgctattt tttgggtatt tgcagtgatg ttcttccctt tctggttttt
     1741 tcatcaaggc agtaattttt gtcgcgcatg taaaagtgga gtctgtattt cagcaatcca
     1801 tcttttagat ttattcctgt tctccgataa aactttttcc attgctgtat tttccttctt
     1861 ttttactttc accatattta tggtctttac ctgtttatgt gtacttcaga aaagagcatc
     1921 tggtagaagt tatcattcag acaacagcgg ttccgctgga ggctcaagtg gcggcggatt
     1981 tagcggcggg ggaggttctt ctggcggtgg cggcgcatcc ggccgctggt aactccacca
     2041 taaaaaatgc cagcccgaag gctggcatta tcaaatcaga taaagtcaga cttatgcctg
     2101 gcctttgatc tctttacgac cgttgtacgg tgctttttcg cccagagctt cttcgatacg
     2161 aatcagctgg ttgtatttag caacacggtc agaacggctc atagaaccag ttttgatctg
     2221 gcctgcagca gtaccaacag ccagatcagc gatggtagca tcttcagttt caccagaacg
     2281 gtgagagata actgcagtgt agccagcatc tttcgccatc ttgattgcag ccagagtttc
     2341 ggtcagagaa ccgatctggt tgaatttgat caggatggag ttagcgatac ctttttcgat
     2401 accttctttc aggatcttgg tgttggttac gaacaggtcg tcaccaacca gctggatttt
     2461 gtcgcccaga actttggtct ggtatgcgaa accgtcccag tcagattcgt ccagaccgtc
     2521 ttcgatagaa acgatcgggt actgtttggt cagttcttcc aggaagtgag tgaattcttc
     2581 agaggtgaac gctttgttgc cttcgccagc cagaacgtat ttaccatctt tgtagaattc
     2641 agaagctgcg cagtccatcg ccagagtgat gtctttgccc agttcataac cagcagcttt
     2701 aacagcttca gcgataacag ccagagcttc agcgttggaa cccaggttcg gcgcatagcc
     2761 accttcgtca ccaacagcag tgttcatgcc tttcgctttc agaacttttg ccaagtgatg
     2821 gaaaacttca gaacccatgc ggatggcttc tttcacagtt ttcgcgccaa ccggctgaat
     2881 catgaattcc tggatatcaa cgttgttgtc agcgtgctca ccaccgttga tgatgttcat
     2941 catcggaacc ggcatggagt atttgcccgg agtgccgttc agttcagcga tgtgctcgta
     3001 cagcggcata cctttagcag ctgcagcagc tttggcgtta gccagagata cagccaggat
     3061 tgcgttcgcg ccgaatttgg atttgttttc ggtgccgtcc aggtcgatca tgatcttgtc
     3121 aatgccagcc tgatctttag catctttgcc aatcagcgcc tgagcgatcg ggccgtttac
     3181 cgcagcaaca gctttggtta cgcctttacc caggaaacgg gatttgtcgc catcgcgcag
     3241 ttccagagct tcacgggaac cagtagaagc acctgacgga gcagctgcca taccgacgaa
     3301 accaccctcc agatgtactt cggcttcaac agtcgggtta ccacgggagt cgatgatttc
     3361 acgaccgatg atttttacga ttttggacat taggttttcc tcaagtcact agttaaactg
     3421 aaactccaga caaacaacgc gtacccaagg tacgcgttgc cgttctaact tttttactta
     3481 cttcgcctga cgtttctgga actcgctggc ggctttcaca aagcctgcaa acagcgggtg
     3541 accatcacgt ggagtagaag taaactccgg atggaactgg caagccacga accacgggtg
     3601 attcggaact tcgatgatct cgaccaactg atcatccccg gaacggccca caacgcgcag
     3661 acctgcatct tcaatctgtt tcaacagcat gttgttgact tcgtaacggt gacgatgacg
     3721 ctcaacaatt gtcggcgcat tgtatagctg gcgaaccagg ctatcgtcaa ccaactggca
     3781 ctgctgtgcg ccgagacgca tggtaccgcc gagatcgctc ttctcgctac gaacttcaac
     3841 gttgccgttt tcatcgcgcc actcggtaat cagcgccaca accgggtact tacagtctgg
     3901 cacaaattct gtagagttgg cgttctccat gttggcaaca tggcgagcgt aatcaattaa
     3961 cgccacctgc atacccaggc aaatgcccag ataaggaata ttgttctcac gcgcaaaacg
     4021 cgcggtcgta atcatgcctt ctacgccacg atagccgaaa ccgccaggta cgaggattgc
     4081 gtccagacct ttaaggattt caacgccgcg cgtttcaaca tcttgtgaat cgatcagttt
     4141 gatgttgacg ctgacacgat tcttcagccc accgtgtttc agtgcttcga tcactgattt
     4201 ataagcatcc ggcagttcaa tgtacttgcc gaccataccg atggtgactt cacttaccgg
     4261 attcgcttct tcgaagataa cctgttccca ttcggacaga tttgcttccg ggcagtttaa
     4321 gctgaatcgt ttacaaatat aatcgtccag cccctgagat ttcaacaggc ccggaatttt
     4381 atagatggaa tcgacgtctt tcagagaaat aaccgctttt tccggaacat tacagaacaa
     4441 tgcaatcttc gcacgttcgt tcgccggtac agcgcgatct gaacgacaaa tcaggatgtc
     4501 aggctggata ccgatggaga gcagctcttt tacagagtgc tgagtcggtt tggttttgac
     4561 ttcaccagac gctgccatgt acggcaccag cgtcaggtgc ataaacagag tgtgctcacg
     4621 gccaatttca acagccatct ggcgaatagc ttcgaggaac ggcaaggatt cgatatcacc
     4681 tactgtaccg ccgatttcta ccagtactac gtcatgacct tcgccacctt ccagcacgcg
     4741 ctctttgatt gcgttagtga tgtgcggaat aacctgcacg gttgcgccga ggtagtcacc
     4801 gcggcgttct ttacgcagaa cgtcagagta gatacgaccc gtggtgaagt tgttgcggcg
     4861 gctcattttg gtacgaatga aacgctcgta gtgccccagg tccaagtcgg tttcagcgcc
     4921 gtcttcagta acgaacactt ccccgtgttg gattgggctc atagtacctg gatcgacgtt
     4981 gatgtaagga tccagtttca tgatggtcac attgaggcca cgggcttcaa gaatggctgc
     5041 gagggaggct gcggcaatgc ctttacccag agaggatacg accccgccgg tcacaaaaat
     5101 atagttcgtt gtcatgctga acctgagaag ttaggttgaa aagacgatgg aataaccaag
     5161 acgggaaagc agtatacccg aacatgacct gtgccacaaa ctttcattat ccctcctctt
     5221 cgccagcgca ctattgaaat caggagtgag aaaatagccc ctttggggta aatgtttttg
     5281 acgcaaatca agcgcttgtc atttaaaaaa tgacacaaat ggcgcttgac cgcgtaattc
     5341 ccttagagat caatttcctg ccgttttacc tgttgccaga cttcttccat tgtttcgagg
     5401 tcaacacctg tcatttccag tccacgcgcg gcaacaatac gctccacttc gcgaaaacga
     5461 cgctcgaatt tttcgttcac tttttgcaat gcgatttctg ctttcgtccc taaatggcga
     5521 gccagattaa ccgtggcaaa cagcaggtcc cccatttcct cctccagttt agcctggtcg
     5581 acaacagcct gccgcgcctc gtacatcacc tcgtcgatct cttcgtagac tttatcaacc
     5641 accgggccaa gcgtcgtcca gtcgaagcca acgttggcgc aacgtttctg gattttttgc
     5701 gcccgcatta aagccggtaa actacgagga atatcgtcca gcgccgaatg ctgcgctttc
     5761 tgcgcgcgct cttcggtttt gatttgctcc caacgggcaa gcacttcact actgttttcg
     5821 gcagaactat cagcaaaaac atgcggatgg cgacgctcta atttatcgct aatagcagcg
     5881 caaatatcat taaagtcaaa gcgcccttct tcctgagcca tttgcgcgta aaacaccacc
     5941 tggaatagca gatcgcccag ttcaccgcga agatcgtcaa aatcttcacg ggcgatggcg
     6001 tccagcactt cgtaggtttc ttcaagggtg taaggcgcaa tggtggcaaa tgtctgctct
     6061 ttatcccacg ggcagccgtt ttccggatcg cgcaggcgct gcataatagt gagcaaacgg
     6121 tcgatttgat tcattgaatt gtcctgaaaa ttgcgggtct gtcaggtgta ataccttcga
     6181 gtaaaagata tcgctggata cggctgacgc catatccagc tatgtaagac taactcccgt
     6241 acaaccgacg cgcgtcgata acatccggca cctggttgag tttacccagc acgcgcccca
     6301 gcacttgcag gttgtaaatc tcaatggtca tgtcgatggt cgccagttgc tgtttggtgt
     6361 cgctacggct ggcaacgcca agcacgttca ccttctcgtt ggcgagaatg gtcgtgatat
     6421 cacgtaacaa cccactacga tcattagcca ccacgcggac caccagcgaa tatccggcgg
     6481 agtagctctc accccatacc gcgtcaacaa tgcgttctgg cgcatgggag cgcagttccg
     6541 ccagttgttc gcaatcggcg cggtgtactg aaataccgcg tccctgggta atgaagccga
     6601 caatctcatc tccaggaatc ggctggcagc agcgcgcgat gtggtgcatc aggttaccaa
     6661 caccttcaac caccacacga ccgttatctt tactgcggtt ttgcggtgtg tagctttttt
     6721 gctgaagctg tttcagcgcg gcggcgtcct gctcttcggc actcggctta ttaaattgcg
     6781 attgcaggaa gttcaccatc tgattgagac ggatatcccc gccaccaatc gccgccagca
     6841 actcgtcgac atcattgaag ttgtaacgcg gcagcagatg tttttctgct tctttcaggc
     6901 tgatccccag atgttccagc tcgtcgtcaa ggatttgccg cccagccaga atgtttttgt
     6961 cacggtcctg tttacggaac caggcgtgaa ttttcgaacg cccacggctg gttgtgacgt
     7021 aaccgaggtt tgggtttaac cagtcacggc tggggttcgg ctgtttctgg gtgataattt
     7081 caatctggtc gcccatttgc agctggtagg tgaacggcac aatgcgcccg ccaatttttg
     7141 ccccgatgca gcggtgtccg acatcactgt ggatgtggta agcgaagtcc agcggcgttg
     7201 atcccgcagg caaatcaacg acatcacctt tcggcgtaaa gacatacacc cggtcgtcaa
     7261 acacctgact acgtacttcg tcgagcattt cgccggaatc agccatctct tcctgccacg
     7321 caatcagttt acgcagccag gcaatccggt cttcatgtcc cgaacgtgcg ccgccagctg
     7381 ccgcgccctc tttatatttc cagtgcgcag caacacccaa ctccgcatct tcatgcatct
     7441 gtttggtgcg gatttggatc tcaacggttt ttccacccgg ccccagaacc acggtatgaa
     7501 tagactgata accgtttggt ttcgggttag cgacgtaatc gtcaaactca tccggcaggt
     7561 ggcgatagtg agtgtgcact atccccagtg cggcataaca atcctgtaaa cgctcggcga
     7621 caatacgtac cgcacgcaca tcaaacagct catcgaaggc gaggttcttt ttctgcattt
     7681 tgcgccagat gctgtagatg tgtttcggac gaccatacac ttcagcttta acgccttcag
     7741 ctttcatctc agcgcgcaga tggccgacaa actcttcgat atagtgttcg cggtcgagac
     7801 ggcgttcatg cagcagtttt gcgatgcgtt tgtattcggt cgggtggaga taacggaagc
     7861 agtaatcttc cagctcccat ttcagttgcc caatcccaag acggttcgcc aacggcgcgt
     7921 agatattggt gcactctttt gccgccagta cacgttcatc ttccggcgca tcttttactt
     7981 cgcgcagatg agcaatacgc tccgccagtt tgatgactac gcagcgaaaa tcatcgacca
     8041 tcgccaataa catccggcga acgttatcga cctgttcgga ggaaacagaa tcagtgtgcg
     8101 tcgctttcag ctggcggatc gccgccatat cacgcacgcc gtgaataagg ttaacaaccg
     8161 acttaccgac gctctcacgc agcacatctt cgctgactac gttggcatca gccagaggga
     8221 acagcagcgc cgcccgcagc gtgtcaatgt ccatacttaa tgtcgagagg atctccacca
     8281 tctcaacacc acgccacaat aacagactgg catccggatg cccctgcgtc tgttgcagac
     8341 aatacgccca ggtttcggct aagcactcac acgacttctg gctggtaata cccagacttg
     8401 cgatccattt ttccggatca aattcaccag ccttattgat atgtgcactt cttaccgcaa
     8461 ccatcgtcct ctcctttagg gaccagacct gccgaaatcg gcaaatcgta actatttaac
     8521 gcgcgagaaa agtaccatcg attccagatg tcccgtgtgt gggaacatat ccagcatcgc
     8581 cagtcgcgca atggtatatc ctgcttttaa taacgcttcg ctatcccgag ccagcgttgc
     8641 agggttacag gatacataaa ctatacgaat aggttccagt tttataattt gctgcataac
     8701 acctgcggca cctgctcgcg ccgggtccag caacacttta tcgaagccgt ttttcgccca
     8761 cggctgcttt gtgacatctt cttcaagatt ttcgtgataa aacgtcacat tctgtaagcc
     8821 attaagacgc gcattctgct ggcctttttc caccagcgcc ggaacacctt ctacaccgac
     8881 cacactggca gcttgtgtcg ccaatggcag tgtaaagttg cccataccgc agaacagatc
     8941 cagtacgcga tcttcaggtt gcacatccag ccattccaac gcacgcgcta ccattttttg
     9001 gttcacaccc gcattgacct gaataaaatc gcgcgggcta aaagttaagc gcaacccgtt
     9061 tgagtcatac cagggcatct caccagagac ggtttcgagt atctcactat cgggggcgag
     9121 atacagatcc aggccttcag aatgcgaaaa gcgttccagt ttttcgcgat ctgccgaact
     9181 tagcggtgcg gtatggcgca aaatcatcag cgtgccgctg gttgcctgta ccagttcaac
     9241 atgaccaaga tggcgcatag cttgtaagct gcccagacat gccctgactt tgggcagcaa
     9301 tgcttcaagt tggggcgcta aaatggggca ttgtttgacg tcgacaatgt cactggagcc
     9361 cgctttgcga aaccccatct gaagttgctg tgttttcggt aagtagttca gacttaaacg
     9421 cgcgcggcgg cgatagcccc agggaacatc ggcgatcact tcagagacat cgtgtttcat
     9481 taatcgggcg agtgccgcac ttttgcttcg ctgctgtaaa tccacgctgg cgtgttgttg
     9541 ctgacagccg ccgcatacgc caaaatgagg acagcgtggc gtttcgcgtt ccgggctatc
     9601 gcttaaccgg cgtacgactt tagcgcgggc atactgtttt ttatcttcag taacagcaac
     9661 ttccgcgttt tcctgcggca ataatccggg gataaataac gttttgccgt tatgtcgcgc
     9721 cacgccctga ccaaaagagt cgaggtcgtt gactgaaacg gttatgatct gacgcgtcgt
     9781 cgtgcgtcgt tttgcagagt agaattgcgc cattggcgag actttctcaa tttaacagtg
     9841 tgaccttaat tgtcccataa cggaactcca tgaccaacta cagcctgcgc gcacgcatga
     9901 tgattctgat cctggcaccg accgtcctta ttggtttatt gctgagtatc tttttcgtcg
     9961 tgcatcgcta taacgacttg cagcgtcaac tggaagatgc cggtgctagc attattgagc
    10021 cgcttgcagt ttctactgaa tatggcatga gcctgcaaaa tcgcgaatct atcggtcagt
    10081 taataagcgt actgcatcgt cgccattccg atattgttcg cgcgatttcg gtttatgatg
    10141 aaaataaccg actctttgtc acctccaatt ttcatcttga tccctcatca atgcagctcg
    10201 gcagcaacgt gccgtttcct cgccagctca ctgtcactcg tgacggcgat attatgatcc
    10261 tccgcacgcc gattatttct gagagttact cccccgacga atcgcccagt agcgatgcca
    10321 aaaatagtca aaatatgttg ggatatattg cgcttgagct ggatcttaaa tcggttcgct
    10381 tgcagcaata taaagagatc tttatttcct gcgtgatgat gctgttttgt atcggtattg
    10441 cgcttatttt tggctggcgc ttaatgcgcg atgtaaccgg tccgattcgc aacatggtga
    10501 ataccgtcga ccgcatccgt cgcgggcaac tcgacagccg ggtggaagga tttatgctcg
    10561 gcgagctgga tatgctgaaa aacggtatca actcgatggc aatgtcgctg gctgcttatc
    10621 acgaagagat gcagcacaat atcgaccagg cgacgtccga tctgcgtgaa acgctggagc
    10681 agatggaaat tcagaacgtt gagttggatc tggcgaaaaa gcgcgcccag gaagcggcgc
    10741 gtattaaatc cgagtttctg gcaaatatgt cacacgagct gcgtacacca ctgaatggtg
    10801 ttattggctt tacccgcctg acgctgaaaa cagaattaac accaacgcag cgcgatcacc
    10861 tgaatacgat tgaacgttcg gcaaataatt tgctggcaat tattaatgat gttctcgact
    10921 tctcgaaact ggaagcaggt aagctgattc tggaaagtat tccattccca ctacgcagca
    10981 cgctggatga agtcattact ctgctggcac attcttctca cgataaaggg ttggaactga
    11041 cgctcaatat taaaagcgac gtgcctgata acgtgatcgg cgacccactg cgattacagc
    11101 aaatcatcac taacctagtg gggaatgcaa ttaaattcac cgagaatggc aacattgata
    11161 ttctggtaga aaaacgtgcg ctgagtaata ccaaagtgca gattgaagtg cagattcggg
    11221 ataccggcat tggtattcct gaacgcgatc aatcgcgctt attccaggcc ttccgacagg
    11281 ctgatgccag tatttcccgc cgtcatggtg gcaccgggct ggggctggtg attacacaaa
    11341 aactggttaa tgaaatgggc ggcgatattt cgttccatag ccagccgaat cgcggttcaa
    11401 ctttctggtt ccacattaat ctcgatctga acccgaacat tattatcgaa gggccatcca
    11461 tccagtgcct cgcaggtaag cgcctggcct atgtcgaacc aaactccgca gcagcgcaat
    11521 gcacgctgga tattttaagt gaaacgccgc tggaagtggt ttatagccca acgttctccg
    11581 cgctgcctcc cgcgcattac gacatgatgt tgttaggcat cgcggtgacc ttccgcgagc
    11641 cgctaacaat gcaacatgag cgattagcga aagcggtatc gatgaccgat ttcctgatgc
    11701 tggcacttcc ttgccatgca caagtcaatg ctgaaaaact caagcaagat ggtatcggcg
    11761 cgtgtctgct gaaaccatta acacctacgc gcctgttgcc tgccctgacg gaattttgtc
    11821 atcacaaaca aaacacgctt ttgcctgtaa ccgatgaaag taagctggca atgacagtca
    11881 tggcggttga tgacaacccc gctaacctga aacttatcgg cgcattgctg gaagatatgg
    11941 tgcaacatgt ggaactttgc gatagcgggc atcaggcagt tgaacgggcg aaacagatgc
    12001 cgttcgattt gatcttaatg gatattcaaa tgccggacat ggatggcatt cgggcctgcg
    12061 aactcatcca ccaactcccg catcagcgac aaacgccggt tatcgcggta acggcgcatg
    12121 caatggccgg gcaaaaagag aagctgcttg gcgcagggat gagcgattat ctggcgaaac
    12181 cgattgaaga agagcgattg cataatttgt tgttgcgcta caagcctggc agcggtattt
    12241 cgtctcgcgt cgtgacgccc gaagtcaacg aaattgtggt gaacccgaat gcgaccctcg
    12301 actggcaact ggcactacgc caggcagcag gaaaaaccga tttagcgcgc gatatgctgc
    12361 aaatgttact cgatttcctg cctgaagttc gcaacaaagt tgaggaacag ctggttggag
    12421 aaaacccgga aggcctggtt gatttgattc ataaactgca tggcagttgc ggctatagcg
    12481 gcgtaccacg tatgaagaat ctctgccagc tgatcgaaca acagctacgt agcggtacta
    12541 aagaagaaga tttggagccg gagctgctgg aactgttgga cgagatggat aatgtcgcgc
    12601 gcgaagccag caaaattctc gggtaacgga cgtcttgtcg agtagcaatc ggctttttat
    12661 gacgctggtt tcagattggt gacaaagtgc ctgtgtttat gccggatgcg gcgttaacgc
    12721 cttatccggc ctacaaaacc aattaaattc aatgaattaa aaaatgatgt agacctgata
    12781 catagcacaa caggctattt tgcgtttagc atcagtctca aaccggctcc agatagagcc
    12841 ggttttggtt ttctgtctta acgcaccatg cacgggcgct tgttatcgaa cgtccagcca
    12901 ggaatcagat actgcattcc catcgcatcg tcacgcgcgc caagcccgtg tttctgatac
    12961 agctcatggg ctttcatcac ttgatccata tcgatttcta cacccagccc cggtttttct
    13021 ggcacctgta ccagcccgcc tttgatctca aacggttctt tggtcaggcg ctgattacct
    13081 tcctgccaga tccagtgcgt atcaatagca gtaattttac ccggtgcagc ggcggcaaca
    13141 tgggtaaaca tcgccaggga aatatcgaag tggttgtttg agtgtgaacc ccaggtcagg
    13201 ccaaattcat ggcacatttg cgccacacgt accgaacctt gcatagtcca gaaatgcgga
    13261 tccgccagcg ggatatcaac ggattgtaac gagagcgtat ggcccatctg ccgccagtcg
    13321 gtggcgatca tattggttgc agtcggcagg ccagtcgcgc gacggaactc tgccatcact
    13381 tcacgcccgg agaaaccttg ctccgcacca cacggatctt ctgcataagc cagcgaacct
    13441 ttcaggtatt taccaatttt aattgcttcg ttcagcgacc aggcaccgtt aggatcgagc
    13501 gtaatacgcg cctgcgggaa gcgttgcgcc agtgccacaa tagactcggc ttcttcttcc
    13561 ccggccagta caccgccttt cagtttgaaa tcgttgaagc catatttttc atacgccgct
    13621 tccgccaggc gcaccaccgc atccggcgtc atcgcttctt catgacgcag gcgataccag
    13681 tcgcatgagt catccggctg gctttgatac ggcagaggtg tggctttgcg attaccgacg
    13741 aagaacagat aaccgagcat ttcgacttcg ctacgctgtt gaccatcgcc cagcagcgat
    13801 gccacgttta cccccagatg ctgccccagc agatccagca ttgccgcttc tatcccggta
    13861 actacatgaa tagtggtacg caaatcaaat gtctgcaaac cgcgcccact agcatcacga
    13921 tcggcaaaag tattacgcac cagcgtcaga acgtttttgt attcacccag cgttttacct
    13981 accaccagcg gaatcgcatc ttccagcgtt ttacggattt tctcgccgcc gggaatttcc
    14041 cctacgccag tgtgaccgga attatctttg ataatcacaa tattacgcgt gaagaacggt
    14101 gcgtgtgcac cactcagatt catcagcata ctgtcatgac ccgccaccgg gatgacctgc
    14161 atttcagtaa caacaggcgt cgtaaattga gaactcatat ttatgtcctt tttcagaatc
    14221 aatgacggcc gaaaacggga cgtttacggt caaaggtcca gccggggatc aggtactgca
    14281 tcggacctgc gtcgttacgc gcaccgcaag gcagacgttt ataggcctca tgtgcctttt
    14341 gtacctgttc ccagtccagt tccacgccca gaccgggcgc atcaggaacg gcaatttttc
    14401 cgtttttaat ctccagcgga tttttggtca ggcgacaatc gccctcctgc caaatccagt
    14461 gggtatcgat agcggtagga ttacccggtg ccgccgcgcc cacatgggta aatatcgcca
    14521 gagagatatc gaaatggttg ttggaatggc agccccaggt cagcccccag tcgtcgcaaa
    14581 gctgcgccac gcggactgca ccggaaagcg tccagaagtg cggatcggca agtggaatat
    14641 ctaccgcatt gagcatcacc gcatgaccca tttcgcgcca gttggtggcg atcatgttag
    14701 tcgcgacggg caagccggtc gcccgtcgaa actccgccat cacttcacgt ccggagaagc
    14761 cctgttctgc gccgcatgga tcttcggcat aggtaagaac atcattcagc cctttgcata
    14821 aagaaatggc ttcatcaagc agccatgcac cgttgggatc aacggtaatc cgcgcatccg
    14881 ggaagctttt cttcaatgca cgaacagtgt cgatttcttg ctcgccaggt aacaccccgc
    14941 ccttaagttt gaaatcttta aagccatagc gatcctgtga ggcttccgcc agacgcacaa
    15001 cggcttcgct gttcatcgct ttctgatggc gcaactgata ccactcatgg ttgcccggcg
    15061 tattttccag ataaggaaga tcggttttgg tccgatcacc tatataaaac agataaccga
    15121 ggacggtaat agtctcgcgt tgcttgcctg gccccaacag ttcgcagacc ggaacattca
    15181 gcgccttacc tagcaggtca agcaaggcgg cttccagcgc cgccacggca ttaacgcgca
    15241 attcaaaagt ccaggcacct ttgccgaagg tctcaaaatc ggctgcctga ttacctttat
    15301 gtacctgctg aaccacttta ttcaggcgcg caacttcctg gcccagaacc atcggaatag
    15361 catcgaccag tgtttgataa atcacctctc cgcccggcgc ttcaccaatg ccggtatgcc
    15421 cggcgttatc ggtgagtacc acgatattgc gggtgaagta tgcgttatgc gcaccaccaa
    15481 tattgagcaa catgctgtca tgtccggcga ccggaatgac tttcatatca gtaataacag
    15541 gactggattg tgtcgccatc ttattgcccc gcgacaggtt tcagctcgat acgcttgata
    15601 tcgcccacca gcaccaggta gctcagtacc gcgattaggg catgaacacc aacataaatc
    15661 agcgccccat taaacgagcc agtcgtgcca acgatataac caattgcgat tggcgtgacg
    15721 atgccagaaa tgttaccgaa catgttgaac tggccaccgg aaagaccgct gatctctttt
    15781 ggcgcggtat ctgccattac tgcccaaccc agcgccccga tgcccttacc gaagaaggcc
    15841 agcgccataa agccgatgat catccactca acgttgacgt agttgcagaa caccatcacc
    15901 atcgacagca acatgcccat tacgatcggt gttttacgcg caatgttcag cgatcccgtg
    15961 cggcgcatca gccagtcgga aataattcca cccagcacac cgccgataaa accgcaaacc
    16021 gccggaacgg aagccacaaa gcccgctttc agaatcgaca tcccacgcgc ctgcaccaga
    16081 taaaccggaa accaggtaat gaaaaagtaa gtcagggcgt tgatacagta ctgaccgata
    16141 taaacgccga tcatcatccg tgacccgagc agctgtttga tctgccccca cttcacgctg
    16201 aacggcactt taactttggt gctttgctga tccatattga tcagcgcgcc acccgcggcg
    16261 atatactcca gctctttctg atttaccccc ggatgttgat ttggctcgtg gatgactttc
    16321 aaccagataa agctgatgac aatgcccaga ccgcccataa agaagaagac gtgtgaccag
    16381 cccacttcat gcgtcagcca gcccataatc ggcgcgaaga tcaccgttgc gaagtattga
    16441 gcggagttaa aaatcgacac cgccgttccc ctttcctgcg ccggaaacca ggccgcaaca
    16501 atgcgactgt tgccggggaa agatggcgct tcagcaagcc cgaccaggaa gcgcagagta
    16561 aacagggcaa cgataatgcc gaatccacta aagatatcga cgaagccttg cagcaaggta
    16621 aacatcgacc agataaagat cgaccagaag tagacgcgtt ttgaaccaaa acgatccagc
    16681 aaccaaccac cagggatctg cccgataaca taagcccatg agaaggcaga gaacacatag
    16741 cccattccca cgggatcaag gccgatatct ttggccattt ccgaaccggc aatagaaagc
    16801 gtagcgcggt cgccgtagtt gaaggatgtg acgataaaca acatcaccac tatccagtaa
    16861 cgagcattcg tgcgtttttc cacactgctc gcagcctgac ttaaagaact cattgttgca
    16921 ctcctgaaaa ttcgcgttag ccacgctcac tctggactgc gacatcgcca ggaaatcaga
    16981 ggtgacgtag ggtgtttttt gccgttttta taggtcgttc gccgaatacg gcgcgtgttt
    17041 atatctggca atagcagtat aaaaagcgcg ccatagcggc tcaccgtgca acaacacaac
    17101 attaatgcgt tcaatgaggc ccgattttgg cattagccct ggacggtgga atccacttca
    17161 cggaaatgaa aacaagaaca agaaaggaag ggtaaaaacg aagaaataaa gaagagtatg
    17221 aaatggatcg cttgactcta ggcaaacgcc agtaaaaatc cgcgctatga agcagttttt
    17281 actggcattt gcctgaaaag attcgattca gcaccgctaa aacgacattt accgctcgct
    17341 gacatatcag gacaacagcg tgccccactg ttcgacccac ggatttgatt ccgtttccgg
    17401 ttccgggttc tcactggcgt caatcaacag catttcgcca acccgctgag cgctctgttc
    17461 ctgcaacaag gcatcgaact gtttgccgcc attgcaaaaa tttacataac tgctgtcgcc
    17521 gagcgcaatc acgccataac gcagattcgg ctggaagccc agactatctt tgattccctg
    17581 aaagagcggc acaatgctat caggaaggtc gccctgcccg gtcgtggacg taaccaccag
    17641 aacatactta tcctgatagg gcagccagtc gcttaattca ggatcttcaa ataccgttgc
    17701 tttgtggccc tgtgcggtca gaattgcttc tgcttcttcc gccactaaca gcgaatttcc
    17761 gtacatggtg ccgacaaaaa taccaatttc cgccataccg ttttctccct ggattaggaa
    17821 cttatctctc catcctgacc cgatggcgca ctgaactcaa ccctttcatt ttcaggaagc
    17881 agaccgcgcc agccaaattg tgataacgcc tgcatccagg tgtcgtccag gcccgcgtga
    17941 atagtcagcg gctcgccagt aaaaggatgc gtcaatgaca actgactggc atgtagcatt
    18001 aaccgctgga ggccaaaatg ctcagcaccg ctgcgattct ggcgtaaatc tccatgtttg
    18061 ctatcgccaa taatcggatg acgcaaatgg gcaagatgtc ggcgaagctg atgtttgcgt
    18121 ccggttttcg gctccagttc caccaggccg tagcgcgtgg tcgggtaacg tccggtcgct
    18181 accggcattt ctacggtcgc cagaccgcga taatgcgtca ctgctggctg cgggccttta
    18241 tcttcgcggg caaatttatc agcgattttg tccagttctt ccaccagtgg ataatccaac
    18301 accgcttctt ccatcaacca gccgcgcaca atcgcatggt aacgtttctg gatttggtgc
    18361 tgttcaaact gttgtgccag cagccgtccg gcctcgctgg ataatcccat caacaacaca
    18421 ccagaagtgg gtcggtccag acgatgagca gtaaaaacat gctgtcctat ctggtcacgc
    18481 acggtttgca tgaccactac tttctcgtcg cgatccagcc agctacggtg aaccagccag
    18541 ccggagggtt tatttaccgc aaccagccat tcatcctgat agagtatttc cagcattagc
    18601 tcgcatcatc cgcaaaaaga gcatccagtt tttccaactc agccagaata agcgcgcgtt
    18661 gcggatggtc cgtcgctagc gccatttcat aatagggtgc aacagcaaaa gcgcccggta
    18721 acggctggtt gttatttaac aaatcgtgca ttcgcgggat cagcccccac tgcaaccact
    18781 ccagtggttc catggtgtcc ataaagaacg gttgggtact attaaattga tgcggctggg
    18841 gttcatcgtt tcgccagtgc tgatgttcac gcagtaacgc ttcaagcgcc tgcaactgga
    18901 gacgaacgcg gtcatgagtg gtcataaagc aacctcaata agaaaaactg ccgcgaagga
    18961 tagcaggaat aaaaaaaggg agcactgtat tcacagtgct cccggttcgt ttcgcagcat
    19021 tccagctact tttgttgctc cctgctcatc cttgacaact tttcctctgg ccttgcggcc
    19081 aatcgttcat cctgaactat tgcttcctgc tcacaccacc ccgatgtgat acttcatcct
    19141 gaagtgtccc tggccttcct gacccaccga atcatcctga ccggttctca ttctccatcc
    19201 tggaggtgtc ctttaacgcg tcctgcgtca tcctcttcgc ttcatccaga agcctttccc
    19261 tgaaacacca tcctggtgtg tcctgcagaa gtgtcatcat cctgatgttc acttcgttgt
    19321 ctgactccct gtcgacgaag atagaatcgt ctttttcgcg aagtcttaca aggcgcttac
    19381 agacaatgcc ttaaggaaaa tttcatatga aagttaagaa ttaacaatca acattttgat
    19441 tttaaatgaa aataacgatt cttatctttt taagtattcg cttatttcct ggcgatctcc
    19501 tacagagtct gtaagagatc tctcacaacc agatggggta tttagattac agaagaggct
    19561 taagttgatt aaggaattcc gcaagatttg aggctaaatg cgtccgttta cgtgttccca
    19621 gcgtctcttt gcacacttca ccggagagat tacacaccga gatgacctct aactcttctt
    19681 ccagcgtcgc gataaatagc gtgggcggca gttttagcct tttctgagtc accagatgac
    19741 caatcagatt ttcctgcacc cgacggaagt cgtcttcact ccaggtttgc agcaatgtca
    19801 gtttgatatc gccaaactgc gcgtgcatat ccccggcaaa ctgagtcgta taaaaggtat
    19861 gaattgtggg ttgtatcaca atatcaaaag cgcgttcaac cgcgtttaca ttttgttccc
    19921 cggtaaacgg ctggggttgc cagtatacgg catcttcggt agttgaaata atgcacggtg
    19981 aaggaacacc gtacagttcc tcacttaacg gccagctttt atgctcttca tgccacgcat
    20041 cgcagtagcg tgcagtaaaa tctttcaggg cttgtgcggt caaatcgtcc actaatttct
    20101 ctcttcactt aaaccagata cacttgtcgt ttagtttatc tggtttatga cggtgaaaca
    20161 tgtcttctta tgcaaaccat caggcacttg cgggcctgac tcttggaaaa tcaaccgatt
    20221 accgggatac ctatgacgcc agcctactgc aaggcgttcc acgcagcctg aatcgcgacc
    20281 cgctgggtct gaaagcggat aacctgcctt ttcacggtac ggatatctgg acgctgtatg
    20341 aactttcctg gctgaatgcg aaaggtttgc cgcaggtcgc tgtcggtcat gttgaacttg
    20401 attacaccag cgtaaatctg attgagtcga agagttttaa gctctatctc aacagtttta
    20461 accagacgcg ttttaataac tgggatgagg tgcgccagac gctggagcgc gacttaagca
    20521 cttgcgctca gggtaagatt agcgtggcgt tatatcgtct tgatgaactg gaaggccagc
    20581 cgataggtca tttcaatggc acttgcattg atgaccagga tatcactatc gataactatg
    20641 aattcactac tgactatctg gagaatgcca ccagcggtga aaaagtagtg gaagagacgc
    20701 tcgtcagcca cctgctgaaa tcaaactgcc tgatcaccca tcaaccagat tggggttcga
    20761 tccaaattca gtatcgtgga cgccaaattg acagagaaaa actgctgcgt tacctggtct
    20821 cattccgtca tcacaacgag ttccacgaac agtgcgtgga acgcatcttt aatgacctgt
    20881 tacgcttctg ccagccagag aaattgagcg tttacgcacg ttatacccgt cgtggcggtc
    20941 tggacattaa cccgtggcgc agtaatagcg attttgtccc atcgaccaca agactggttc
    21001 ggcaataaat tttttctcaa ttttgcgtgc tggattcacg cagaaggttg tgaaaggtca
    21061 tcaggcaggg ctattgtaat caaagggaat gacgatattc gtcccataag gagtttttct
    21121 tgattacaca tattagcccg cttggctcca tggatatgtt gtcgcagctg gaagtggata
    21181 tgcttaaacg caccgccagc agcgacctct atcaactgtt tcgcaactgt tcacttgccg
    21241 tactgaactc cggtagtttg accgataaca gcaaagaatt gctgtctcgt tttgaaaatt
    21301 tcgatattaa cgtcctgcgc cgtgaacgcg gcgtaaagct ggaactgatt aatcccccgg
    21361 aagaggcttt tgtcgatggg cgaattattc gcgctttgca ggccaacttg ttcgcggttc
    21421 tgcgagacat tctcttcgtt tacgggcaaa tccataatac cgttcgtttt cccaacctga
    21481 atctcgacaa ctccgtccac atcactaacc tggtcttttc catcttgcgt aacgctcgcg
    21541 cgctgcatgt gggtgaagcg ccaaatatgg tggtctgctg gggcggtcac tcaattaacg
    21601 aaaacgagta tttgtatgcc cgtcgcgtcg ggaaccagct gggcctgcgt gagctgaata
    21661 tctgcaccgg ctgtggtcca ggagcgatgg aagcgccgat gaaaggtgct gcggtcggac
    21721 acgcgcagca gcgttacaaa gacagtcgtt ttattggtat gacagagccg tcgattatcg
    21781 ccgctgaacc gcctaacccg ctggtcaacg aattgatcat catgccggat atcgaaaaac
    21841 gtctggaagc gtttgtccgt atcgctcacg gcatcattat cttccctggc ggtgtgggta
    21901 cggcagaaga gttgctgtat ttgctgggaa ttttaatgaa cccggccaac aaagatcagg
    21961 ttttaccatt gatcctcacc ggcccgaaag agagcgccga ctacttccgc gtactggacg
    22021 agtttgtcgt acatacgctg ggcgaaaacg cgcgccgcca ttaccgcatc atcattgatg
    22081 acgccgctga agtcgctcgt cagatgaaaa aatcgatgcc gctggtgaaa gaaaatcgcc
    22141 gtgatacagg cgatgcctac agctttaact ggtcaatgcg cattgcgcca gatttgcaaa
    22201 tgccgtttga gccgtctcac gagaatatgg ctaatctgaa gctttacccg gatcaacctg
    22261 ttgaagtgct ggctgccgac ctgcgccgtg cgttctccgg tattgtggcg ggtaacgtaa
    22321 aagaagtcgg tattcgcgcc attgaagagt ttggtcctta caaaatcaac ggcgataaag
    22381 agattatgcg tcgtatggac gacctgctac agggttttgt tgcccagcat cgtatgaagt
    22441 tgccaggctc agcctacatc ccttgctacg aaatctgcac gtaatctccc ctcccc
//
LOCUS       DAETPZ010000061        21644 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.061, whole genome shotgun sequence.
ACCESSION   DAETPZ010000061 DAETPZ010000000
VERSION     DAETPZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.061"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..157
                     /locus_tag="K0W59_002673"
     CDS             <1..157
                     /locus_tag="K0W59_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBI3023653.1"
                     /translation="LSIEMNKLKQARYSIGIAMSEEKYSGIIGALRGKYINCLVTNSS
                     TAELLLK"
     gene            227..1033
                     /locus_tag="K0W59_002674"
     CDS             227..1033
                     /locus_tag="K0W59_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI3023654.1"
                     /translation="MQTWLNLQDKIIIVTGSASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            1043..1450
                     /locus_tag="K0W59_002675"
     CDS             1043..1450
                     /locus_tag="K0W59_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HBI3023655.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIA
                     VKLEKLASIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            1450..1944
                     /locus_tag="K0W59_002676"
     CDS             1450..1944
                     /locus_tag="K0W59_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HBI3023656.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            2010..2807
                     /locus_tag="K0W59_002677"
     CDS             2010..2807
                     /locus_tag="K0W59_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBI3023657.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            2818..3606
                     /locus_tag="K0W59_002678"
     CDS             2818..3606
                     /locus_tag="K0W59_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBI3023658.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            3658..4887
                     /gene="sorE"
                     /locus_tag="K0W59_002679"
     CDS             3658..4887
                     /gene="sorE"
                     /locus_tag="K0W59_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HBI3023659.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            5269..5958
                     /gene="pepE"
                     /locus_tag="K0W59_002680"
     CDS             5269..5958
                     /gene="pepE"
                     /locus_tag="K0W59_002680"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HBI3023660.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(6049..7680)
                     /locus_tag="K0W59_002681"
     CDS             complement(6049..7680)
                     /locus_tag="K0W59_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBI3023661.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(7900..11583)
                     /gene="metH"
                     /locus_tag="K0W59_002682"
     CDS             complement(7900..11583)
                     /gene="metH"
                     /locus_tag="K0W59_002682"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBI3023662.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMVDYQMESLSAE
                     INFAAAKLARARADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            11783..12607
                     /gene="iclR"
                     /locus_tag="K0W59_002683"
     CDS             11783..12607
                     /gene="iclR"
                     /locus_tag="K0W59_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HBI3023663.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            12924..15108
                     /gene="espL4"
                     /locus_tag="K0W59_002684"
                     /pseudo
     CDS             12924..15108
                     /gene="espL4"
                     /locus_tag="K0W59_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(15077..16813)
                     /gene="aceK"
                     /locus_tag="K0W59_002685"
     CDS             complement(15077..16813)
                     /gene="aceK"
                     /locus_tag="K0W59_002685"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HBI3023664.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPVLMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(16997..18301)
                     /gene="aceA"
                     /locus_tag="K0W59_002686"
     CDS             complement(16997..18301)
                     /gene="aceA"
                     /locus_tag="K0W59_002686"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HBI3023665.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(18331..19932)
                     /gene="aceB"
                     /locus_tag="K0W59_002687"
     CDS             complement(18331..19932)
                     /gene="aceB"
                     /locus_tag="K0W59_002687"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HBI3023666.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGCGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     LREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(20201..21130)
                     /gene="metA"
                     /locus_tag="K0W59_002688"
     CDS             complement(20201..21130)
                     /gene="metA"
                     /locus_tag="K0W59_002688"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBI3023667.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            21287..21577
                     /locus_tag="K0W59_002689"
     CDS             21287..21577
                     /locus_tag="K0W59_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023668.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5339 a         5549 c         5469 g         5287 t
ORIGIN      
        1 gctctctatc gaaatgaata aattaaagca ggcgcgatat tccattggca ttgccatgag
       61 tgaagaaaaa tacagcggaa ttattggtgc actgcgtgga aaatatatta attgtctggt
      121 aacgaatagc agcacagctg aactattact gaaataaaaa tcttcccgat gtttaccgta
      181 ataaaaatta cggcgggtcc ctattatcta aaatcaggag tctgttatgc aaacgtggtt
      241 aaatttgcag gataaaatca ttattgtcac cggcagcgca tccggtattg gtctggcgat
      301 tgtagaggaa ttattagcac aaggcgcgaa tgtacagatg gtcgatattc acggtggcga
      361 tggtcaatat gaaggtcata aaggctatca gttttggccg accgatattt ctagcacaaa
      421 agaggtaaat catacggtag cggaaattat ccagcgtttt ggtcgcatcg acggtctggt
      481 caataacgcc ggggtcaatt tcccgcgtct gctggtcgat gagaaagcgc ctgccgggca
      541 gtatgaactc aacgaagctg cattcgaaaa aatggtcaat atcaaccaga aaggtgtttt
      601 tctgatgtcg caggcggtgg cgcgacagat ggtcaaacaa catgatggcg tgattgtgaa
      661 tgtttcctca gaaagtgggc tggaaggctc agaaggacaa agctgttacg ccgctaccaa
      721 agccgcgctc aatagcttca cgcgctcctg gtcgaaagag ctgggtaaac acggtatccg
      781 tgtggtcggt atcgcgcccg ggattctgga aaaaaccggg ctacgcacgc cggaatatga
      841 agaggcgctg gcgtggacgc gcaatatcac cgtcgagcag ctgcgtgaag gctataccaa
      901 aaacgccatt cctattgggc gcgccggaag attagcagaa gtagctgatt ttgtttgtta
      961 tctgttgtct gaacgcgcca gctacatcac cggagtaacc actaacattg cgggcggcaa
     1021 aacgcgcggc taaggaggct ttatggtcaa cgccattttt tgtgcccacg gcaaattagc
     1081 ttgcgccatg ctggaatcgg ttcagatggt ttacggcaac gccaatgttg aagccgttga
     1141 atttgtgccc ggtgaaaacg ccggagacat tgcggtaaag ctggaaaagt tagcaagcat
     1201 tcacaatcaa gatgagtggc tgattgccgt ggatttacag tgcggcagcc cgtggaatgc
     1261 ggcagcaatg cttgcgatgc gcaatcctcg tttgcgggta atcagcggcc tttctctgcc
     1321 gctggcgctc gaactggttg ataaccagga cagcatgaat gttgatgaac tgtgtgaaca
     1381 cctgacgcaa atcgcaaagc aaacctgcgt tgtctggaaa caactggcaa cgacagagga
     1441 ggatttctga tgaacattac gctcgcccgc attgatgacc gtctgatcca cggccaggtc
     1501 actaccgtct ggtcgaaagt ggctaacgcc cagcgcatta ttatctgcaa tgacgaagtt
     1561 tataacgatg aagtgcgccg gacattattg cgtcaggctg cgccaccggg aatgaaggtt
     1621 aacgtggtca atattgaaaa agccgtcgcg gtttatcaca acccgcaata tcaggacgaa
     1681 accgtttttt atcttttcac tcgcccacag gatgccttag cgatggttcg ccagggggta
     1741 aaaataggca ccctgaatat tggcggcatg gcctggcggc ccggtaaaaa acagttaacc
     1801 aaagcggttt cattagatga tgatgatatt aacgcatttc atgagttaaa taatcttggt
     1861 gtcatactgg atttacgcgt cgtagcgtca gatccctcaa tcaatattat cgacaaaata
     1921 aacgaacagt taatcgcaaa ttaaataata gcgcctgtgt acatatgctt taacaggcag
     1981 ggatgacgac acttaaaagg tgacaaatca tggaaataag taccctacaa atcattgcta
     2041 tatttctttt ttcctgtatt gccggaatgg gcagcgtgct ggatgaattt cagactcatc
     2101 gcccgttaat tgcctgtacg gtgattggtt taattctcgg tgatttaaaa accggaataa
     2161 tactcggcgg tacgctggaa ttgatagctc tcggctggat gaacgtcggc gcggcgcaat
     2221 ctccggattc tgcactcgcc agcataatct ccgccattct ggttatcgtt ggtcagcaaa
     2281 gcatcgccac cggaatcgcc atcgcgttgc ctgtggctgc ggcaggccag gtgctgacgg
     2341 tgtttgcccg taccatcacc gtagtgttcc agcacgcggc ggataaagca gcagaagagg
     2401 cgcggtttcg cactctcgat attctgcatg tctccgcgct tggcgtgcag gcgctgcgcg
     2461 tcgccattcc ggcactgatt gtctcactgt tcgtcagcgc cgatatggtc agcaatatgt
     2521 taaacgccat tcccgaattt gtgacccgag gactacagat tgctggcggt tttatcgtgg
     2581 tggtcggtta cgccatggtg ctacgtatga tgggcgtgaa atatttgatg cctttctttt
     2641 tcctcggttt cctcgcaggt ggctacctcg atctcagcct gctggcgttc ggtggcgtcg
     2701 gcgtgatcat ggccctggtc tacatccagc taaatccaca gtggcgtaaa gctgaacctc
     2761 aactccaggc caccgcctcc accgcccttg accaacttga cgattaacgg agccatcatg
     2821 gaacagagaa aaattacacg cagcgatctg gtgagcatgt ttctgcgctc caacctgcaa
     2881 caggcgtcct ttaactttga acgtattcac gggctgggct tttgctacga catgatcccc
     2941 gccatcaagc gactttaccc attaaaagag gatcaggttg cggcgctcag gcgacacctg
     3001 gtgttcttca ataccacgcc agccgtatgt ggcccggtca tcggcgcgga aattgatgac
     3061 ggtaccatca acggcatcaa agtcggtctg atgggaccat tggcaggagt tggcgatcca
     3121 ctggtctggg gaacgctgcg cccgattacc gccgcgctcg gcgcatctct ggcactttcg
     3181 ggcaacattc tcggcccgct gctgttcttc tttattttca acgcggtgcg tctggcgatg
     3241 aagtggtatg gcctacagct cggctttcgc aaaggggtga atatcgtcag cgatatgggc
     3301 gggaatgtgc tgcaaaaact caccgaaggc gcgtcgattc tcggactgtt tgtgatgggc
     3361 gtgctggtga ccaaatggac gtcaatcaac gtaccgttgg tggtttcaca aacgcatgcc
     3421 gccgatggct ccaccgtcac catgaccgtg cagaacattc tcgaccaact ttgccctggt
     3481 ttgctggcgc tcggtctgac gctactaatg gttcgtctgc tcaacaaaaa aattaacccg
     3541 gtatggctga ttttcgccct gtttggctta gggattatcg gcaatgctct gggcttcctg
     3601 tcctgattct tcgccccggc acgactgccg aggccatcgc tcaacatgag gtggtttatg
     3661 aaaacaacag ctctgcgtct ttatggtaaa cgtgatttac gcctggaaac ctttgacctt
     3721 cctgaaatgc aggaggatga aatcctcgca acggtggtca ctgacagcct gtgcctctct
     3781 tcctggaaag aggccaatct gggtgaaaac cataaaaaag tacccgacga tgtggcgacc
     3841 aacccaatca tcatcggcca cgaattttgc ggcgatattc tggccgtggg taaaaagtgg
     3901 cagcacaaat tccagccggg tcagcgttat gtgattcagg ccaacctgca actccccgac
     3961 cgcccggatt gccccggcta ctccttcccg tgggtaggcg gcgaagcgac gcatgtggtt
     4021 attcccaacg aggtcatgga acaagattgc ctgctggcat acgacggcga aacctatttt
     4081 gaaggctcac tggttgaacc gctttcctgc gtgattggcg cgttcaacgc caactatcat
     4141 cttcaggaag gtagttataa ccacacgatg gggattcgcc cgcaagggcg cacgctgatc
     4201 ctcggcggca ccggaccaat gggactgttg gcgattgatt atgcgctgca tggaccagtt
     4261 aacccgtcgc tgctcgtcat taccgatacc gacaacgaca aattgagtta tgcgcgcaag
     4321 cactatccat cagaaccgca aacactgatt cattatctca atgccaccga tgcagcattt
     4381 gatacgttaa tggcgctgag tggcggtcac ggcttcgatg atattttcgt ctttgtgcct
     4441 aatgaaggac tggtgactct cgcctcttcc ttgctggcga cagatggttg cctgaatttc
     4501 ttcgccggac cgcaggataa acatttcagc gcgccaatta atttctacga tgtgcattat
     4561 gcatttaccc actacgtggg cacgtcaggc ggcaataccg acgacatgcg cgcggcggtc
     4621 aaactgatcg aagagaaaaa agtgcaggcc gcaaaagtgg taacacatat tcttgggctg
     4681 aatgccgcgg gcgaaaccac gcttgaattg cctgccgtcg gcggcggcaa aaagctggtg
     4741 tataccggga aatacctgcc gctgacgtca ctcacgcaga ttcaggatca agcactggcg
     4801 gcgattctgg cgcgtcatca ggggatctgg tccggtgagg cggagcaata cctgctcgcc
     4861 catgcagagg caatttccca tgattaatcg cgctacttaa ctctgcatgt cgctggctgg
     4921 tcgccccagc aacttcgcga ccggatgtgc ggctgattgc tgaagcagca caatttgcac
     4981 gagaaggata aaaaatatca gcgccttaag gggcgctcaa attggtggca aagtacacat
     5041 tgtttatgcc ggatgcggaa tgaagcctta ttcggtctac ataattttga aaatccaata
     5101 tattgcagtt atctggtagg ccaggtaagc gtagcgcatc aggcaatgct gtgtttgtca
     5161 tcagtctcag cgcccggtgc aatacgtgac atcgtgagcc atgtcagtaa gtggttaaat
     5221 tcgcagtggt agcatgcctt ccgtgatttc ttactaaccg gagaagtaat ggaactgctt
     5281 ttattgagta actcgacgct gccgggtaaa gcctggctgg aacatgcact gccgctaatt
     5341 gctgaacagt tgcagggtcg ccgctcagcg gtgtttatcc ctttcgctgg cgtaacgcag
     5401 acctgggatg attacacaga gaaaacggct gcggttctcg ctccgctggg tgtttctgtc
     5461 accggtattc atagcgttgt cgatcccgtt gccgcgattg aaaatgcgga aattgtgatt
     5521 gtcggcggcg gtaatacttt ccagttgctg aaacagtgcc gcgagcgcgg gctgctggct
     5581 ccaattactg acgtggttaa acgtggcgcg ctgtatattg gctggagcgc aggcgctaac
     5641 cttgcttgcc caaccattcg taccaccaac gatatgccga ttgtcgatcc gcaaggtttc
     5701 gatgcgctaa atctgttccc gttgcaaatc aacccgcact tcaccaacgc gctgccggaa
     5761 ggccataaag gtgaaacccg tgagcagcgt attcgcgaac tgctggtcgt cgcgccagaa
     5821 ctgacgatta ttggtctgcc ggaaggtaac tggatcacag tgagtaaagg tcacgctacg
     5881 ctgggtggcc cgaacaccac ttatgtgttt aaggctggtg aagaagcggt tccgctggaa
     5941 gctggtcacc gtttttaagt gaattgccag atgcatgatc cggcatcttg ttcatagtgc
     6001 ctgatgcgac gcttgacgcg tcttatcagg cctacaggat tcacaatatt aatactcgtc
     6061 ccgtccttca tcttcatccg gctgttccag cacactgtaa gccaccgaac aaaacagcga
     6121 gttcaggcgc tgcatatctc ccagtaagcc taaatgtagc gaactggttt caatgctctg
     6181 cacgttttgc tgatgcaggc gatcgacgtg ggcgtgggaa tagcggcgat taagaatgcg
     6241 aaaacgatgt ttgctgcgac gcaaacgacg agcgctggtg acatcgccag agaagaacac
     6301 cgacattgcc agctttaaat tactgagcaa ttgctcatag agcgcatcca gttccttcaa
     6361 cccatcaagc gaaaatgccc gccgtgctgc cagcgattta tcggcaattt cgctgcccat
     6421 gcgctcgacg atatcggagg cctgttcaag gttgagcgac atttcgatga tctccgccca
     6481 gcggcgcgac tcttcctccg ccaactcttc ttttggcatc cgcgccagat acagcttaat
     6541 ggcggtgtag agaacgttga tatcatctgc cagcttacgc agctctttct cctgccgtgg
     6601 ctcgccgtgc atcactttat tcagcccttc catcatctgt tccatggcgt cgccaatgcg
     6661 cagggtttcg cgcgcggcgt tcgccagagc aagcgtgggc gtgtccagcg cgctgacatc
     6721 cagatgttta ggccgtagct gggtatccag ttccggttca tcgcgaataa tcgttttgca
     6781 aaaccgtgcc atcgggtcaa caaatggcag catgaccagg caacgtacaa ggttgtagaa
     6841 gacgtggaaa tagatcacca gttccgcttt tggcaatgac agcttcccca ttgtctctgc
     6901 cagcaaatgg acaaacggca ggataatcag gctacccacc agcttaaaca gcagactacc
     6961 cagcgcgaca cggcgggctg cggcattggc ggcactgttg ttgagcatcg ccagcaggcc
     7021 ggaaccgagg ttagctccaa tcaccagaca gagcgccacg gggaaggaga taatgcctgc
     7081 agcggtcaga gtcgcggtca gcagtacagc agcaaggctg gagtagctga taatggcgaa
     7141 catcgcgcca atcagcgcat ccagcagaat atcgccggtc agcgaggcaa agatcacctg
     7201 aacgccgttt gcctgggtga tcggcgttac ggcctgcaca atcaactcca gcgccagcaa
     7261 aatcagccca agaccaatac cgacgcggcc cagttgcccg gcgcgtgact gtttgcgtcc
     7321 gaggaaaaaa atcacgccga taaaaataag taacggtgac agccaggata agtcgaaggt
     7381 gagaatacgc gccattagcg ccgtcccgac atctgccccc agcacaatga ccagagccgg
     7441 tgcgagggct accagatcct gagcgacgaa cgaggtcacc agcatggtgg tggcattact
     7501 gctctgtacc agtgcggtaa cgccgatccc cgcgcaaaag gcgagcggct ttttttcgac
     7561 gctccggcta aggacagtac gcaaacgcgc gccgaagacg cgcattacgc cggttcgaac
     7621 aatatgagtc ccccagacca gcagggcgac ggcagaaagc aggtgaagca gcgttaacac
     7681 gagttcaggt tctccttatc gttatatgtt cctgaagcaa ttgaatgacc aaaggaccgg
     7741 aaacacgctg actggcatct ttactgtgcc ttttgacatt cgtttttccg gctaagatta
     7801 ttccagcctg tttccagtat aagtgtttca tattaataaa gagacagggc actcattgag
     7861 cgccctgttt gttgtaagga aaagtgacag atttgtgaat cagtccgcgt cataccccag
     7921 attcggtgcc agccagcgct caacatcgga gacgctcata cctttacggc gggcatagtc
     7981 ttcaacctga tcgcgctgaa tttgcgccac cgcgtagtac ttgctgtccg gatgactgaa
     8041 ataccaaccc gaaaccgatg caccaggcca catagcgaaa gattctgtga gtttcatgcc
     8101 agtatgtttt tccacttcca gcagctccca gatggtggct ttttccgtat gttccgggca
     8161 ggccggatag cccggtgccg gacggatgcc ctggtagttt tcgcggatca gttcttcgtt
     8221 gctgaggttc tcgttcggcg catagcccca gtagacttta cgcacacgct catggagata
     8281 ctccgcaaag gcttcggcta aacggtcggc aagcgctttc accatgattt tgttgtaatc
     8341 atcgtgctgc gcttcaaagg catcagccag tgcgtcctct tccagcccgc cagtcacggc
     8401 aaatgcgccg atgtaatctg ctttaccaga aagcttcggc gcaacgaagt cagcgagaca
     8461 gtagttagcg aatcccgttt tttcggtctg ttgacgcaga tggtggctga cgttgatcac
     8521 atgggtgcgc gtttcgtcac ggtagatttc aatgtcatcg cccacacggt ttgccgggaa
     8581 caggcccacc acgccacgcg gattcagcgt tttctcggcg cttaatttat ccagcatgtc
     8641 gttggcgtct ttaaacagcc gctgcgcctc aacgcccacc acttcatctt ccagaatgcg
     8701 cggatacttc ccggccagcg accaggtcat aaagaacggt gtccagtcga tgtaattacg
     8761 cagcgtttcg atgctggctt ccacttcctg cacgccgaga cggtgcgcca ccggcggcgt
     8821 gtaagcctgc cagtcaaatg cgaaatcgtt atcgcgcgcc gcttccagcg tgaccggtgg
     8881 tgtgcgtggt ttcttgcgcc cgtgctgaat acgcacggtt tcgtactcct tgcaggtacg
     8941 agcgacaaaa tcatcacgct gagtatcgga aagcagcgcc gctaccacac caacggtgcg
     9001 cgaggcattc tgcacataca ccgtagggcc gctgtagttc tgctcgattt tcaccgccgt
     9061 gtgcgctttt gaggtggtcg cgccgccaat cagtaacgga atggtgaagc cctgacgctc
     9121 catctctttc gccacgttaa ccatctcgtc cagcgacggc gtgataagcc ccgaaaggcc
     9181 aatcaaatca gcattcactt ctttggcgat acggagaatt ttttccgcag gcaccataac
     9241 gccgagatcg acaatttcgt agttgttaca ttgcagcacc acaccaacga tatttttacc
     9301 gatgtcgtgg acatcgcctt tcacggtggc gatcaccatc ttgccgttgg ttttaccctg
     9361 ctctttactg gcttcaataa acggttcgag atacgccact gcctgtttca tgacgcgcgc
     9421 cgatttgacc acctgtggca ggaacatttt gccttcgcca aacagatcgc cgaccacgtt
     9481 catgccgtcc atcaacgggc cttcaatcac ttcaatcggg cgcgtagcct gctggcgggc
     9541 ttcttcagta tcctgctcga taaactcggt tatgcctttg accagcgagt attccagacg
     9601 tttattcacg tcccacgagc gccactccgc ttgctgggcg ttagcagtgt cgtccgcttt
     9661 actgccgcga tatttctcgg caagctccag taaacgctca gtgccatcgt cacgacgatt
     9721 aagaatcaca tcttccaccg cgtcgcgcag ttcagcgggt aggtcgtcgt aaatcgccag
     9781 ttgcccggcg ttgacgatcc ccatatccat gccattgcga atagcgtagt agaggaacac
     9841 tgcgtgaatg gcttcgcgca ccggatcgtt gccacggaat gagaaagaaa cgttagatac
     9901 gccgccggaa atcagcgcgt gcggcagttc gcgtttgatg tcttcacacg cgccgataaa
     9961 gtcctgcgcg tagttgttgt gctcttcaat accggtggcg accgcgaaga tgttcgggtc
    10021 gaagatgata tcttctggcg ggaagccaac ctcttcggtg aggattttgt acgcccgacg
    10081 gcaaatctcg attttccgtg cgcgagtatc ggcctgtccc tgttcgtcaa aggccatcac
    10141 caccactgcc gcaccgtagc gacgcaacaa tttcgcgtga tggataaagg cgtcgacgcc
    10201 ctctttcatc gagatagagt taacaatgcc tttgccctgg atacacttca gacctttttc
    10261 aatgacatcc cattttgagg agtcgatcat aatcggcacg cgagcgatat ccggttcacc
    10321 ggcaatcaga ttgagaaaac gcaccatcgc cgcttcggca tcgagcatcc cttcatccat
    10381 gttgatatcg ataatctgcg cgccgttttc cacctgctga cgcgccacat ccagcgcctc
    10441 gctgtatttc tcttctttga tcaggcgctt gaacttagca gagccggtga cgttggtgcg
    10501 ttcacccacg ttcacaaaca ggctatcgtc gccaatgttc agcggctcca ggccagacaa
    10561 acggcaggct acgggaattt ccggcagttt gcgcggcgct aatccttcta ctgcacgact
    10621 catcgctgca atatgttgcg gcgtggtccc gcagcaacca ccgacgatat tgagaaagcc
    10681 cgcttgcgcc cattcacgta tctgttttgc catcgtgtcg gcgtcgagat cgtattcacc
    10741 aaaggcgtta ggcagcccgg cgttcgggtg ggcggtgacg tagcattccg caatccgtga
    10801 tagttcctgc acgtactggc gcagttcatc gggccccagc gcacagttca ggccaaaggt
    10861 cagcgcttcg gcgtggcgca atgagttgta aaatgcttcg gtggtctgcc cggagagcgt
    10921 gcgcccggag gcgtcggtga tggtgccgga gatcataatc ggcagctcaa cgcccagcgc
    10981 ttcaaactcc gttttcaccg caaagaccgc cgctttggcg ttaagggtgt cgaaaacggt
    11041 ttcaatcagg atcagatccg caccaccttc caccagcgct ttggtggatt cgcgataggc
    11101 cgccaccagc tggtcaaaag tgatgttgcg gaatgccgga tcgttgacgt ccggagaaat
    11161 agacgccgtg cggttggttg ggccgagaac accggcaacg tagcgcggtt tctccggcgt
    11221 gcgcgcggtc cactcgtcag cacgggcgcg cgccagtttc gccgccgcaa agttgatttc
    11281 cgccgacagg gattccatct ggtaatccac catcgcaatg gtcgtggagt tgaaggtgtt
    11341 ggtttcgatg atatccgcgc ccgcttcaaa gtaggcgttg tggatagcgg cgatcacttc
    11401 cggtttactg agtaccagca ggtcgttgtt gcctttgagg tcgcatggcc agtcggcaaa
    11461 gcgttcacca cgaaaatcgg cttcgttcag tcgatagctc tggatcatgg tgcccatacc
    11521 gccgtccaac accagaatac gttcatttaa ctgcgcacgc agttgttcca ctttgctgct
    11581 cacacttgct cccgacacgc tcaacccaga tttaataaaa attcaacaaa ccatactggc
    11641 ataaacgcat ctgtggtaaa agcgaccacc acgcaacatg agatttgttc aacattaact
    11701 catcggatca gttcagcaac tattgcatta gctaacaata aaaatgaaaa taatttccat
    11761 gatacagaaa aaggaggctg tcatggtcgc acccattccc gcgaaacgcg gcagaaaacc
    11821 cgccgttgcc accgcaccag cgactggaca ggttcagtct ttaacgcgtg gcctgaaatt
    11881 actggagtgg attgccgaat ccaatggcag cgtggcactc acagagctgg cgcaacaagc
    11941 cgggttaccc aattccacga cccaccgcct gctaaccacg atgcaacagc agggtttcgt
    12001 gcgtcaggtc ggcgaactgg gacactgggc aatcggcgca catgccttta tggtcggcag
    12061 cagctttctc cagagccgta atttgttagc aattgttcac cctatcctgc gcaatctaat
    12121 ggaagagtct ggcgaaacgg tcaatatggc ggtgcttgat caaagcgatc acgaagcgat
    12181 tattatcgac caggtacagt gtacccacct gatgcgaatg tccgcgccta tcggcggtaa
    12241 attgccgatg cacgcttccg gtgcgggtaa agccttttta gcccaactga gcgaagaaca
    12301 ggtgactaag ctgctgcacc gcaaagggtt acatgcctat acccacgcaa ctctggtgtc
    12361 tcctgtgcat ttaaaagaag atctcgccca aacgcgcaaa cggggttatt catttgacga
    12421 tgaggaacat gcgctggggc tacgttgcct tgcagcgtgt attttcgatg agcaccgcga
    12481 accgtttgcc gcaatttcta tttccggacc gatttcacgt attaccgatg atcgcgtgac
    12541 cgagtttggc gcgatggtga ttaaagcggc gaaggaagtg acgctggcgt acggtggaat
    12601 gcgctgactt tttctggcgg gcagaggcaa tattctgccc atcatacctg agtggcaata
    12661 gaataagggt gtctgttaat cgcattgacg ccaaaataac ttaatgtcat acacttcaca
    12721 cacttttatt aactcagcat tatttttaaa catcaaacca cttaattatt aacaaaatcg
    12781 caagatctca ttccaccgaa acttttacgg ctgcattatg agattgagcg atttatgata
    12841 tttttttagt tgtaatcata ttaaattttt tcactgttct gatagttaaa attcaagaca
    12901 tcaataaaca atgagatatt taaatgatta ctcgtattcc tcgtagttct ttctctgcaa
    12961 atattaataa tacagcccag acaaatgaac accaaaccct gagtgaattg ttttataaag
    13021 aactcgagga taaattttct ggcaaggagc tggcgacgcc tctattaaaa agcttctcag
    13081 agaactgtag acaaaatgtt cgtcatatct ttagcaacaa ggattttgtc attaaatttt
    13141 ccacgtccgt cttacaagct gataagaaag aaattacgat aattaataaa aacgaaaaca
    13201 cgacactcac tcaaaccatt gctacaatat ttgaagaata cctaatggaa attttacctc
    13261 aacgctcaga cactcttgat aaacaagaat taaacctaaa atcagataga aaagaaaaag
    13321 aattcccaag aattaaactt aatggtcaat gttattttcc ggggcgcccc aaaaccgtat
    13381 agtataccga cacattgctg cacaatatat taatgatatt tatcagaatg ttgattacaa
    13441 accccatcaa gatgattact cttcagctga aaaatttctc actcacttca acaaaaaatg
    13501 caaaaaccag actttggcgt tgatttccag ccgtcctgag gggcgttgcg ttgctgcctg
    13561 cggtgatttc gggctagtta tgaaagcata ttttgacaag atggaatcaa atggcatcag
    13621 tgttatggca gccatattac tggtggataa ccatgctttg acggtccggc taagaataaa
    13681 gaacacaact gaaggatgta cccattacgt gatttcggtt tatgatccta atgtaactaa
    13741 cgataaaata agaattatga gcgaaagcaa agaggatatt aaacactatt ctctgatgga
    13801 ttttatgaat gtagattata gcctcctgaa atggtcaaat gatcatgtta ttaaccaatc
    13861 tgttgcaata attccagcac ttccgaaaga acagctattg atgttaaaag gaactgtgga
    13921 tgaaataacc cctccattat caccagcaac gatgaatttg ctaatggcaa ttggtcagaa
    13981 tcaccaactt acgcaactga tgattcagct ccagaaaatg ccagaactac atagaacaga
    14041 aatgttgact gcctataata gtattaacct ccccggttta tatttggcta taaattatgg
    14101 caatgcggat atcgttgaga ctattttcaa ttcattgtca gaaacaggat atgaagggtt
    14161 actctcgaaa aaaaatctca tgcatattct ggaggcaaaa gataaaaatg gtttttctgg
    14221 attattttta gcgatatcac gtaaggataa aaatgttgta acctcgattc tgaacgtctt
    14281 acctaaactg gccgcaacac atcatttaga taatgaacag gtgtataaat tcctgagtgc
    14341 caaaaataga acgtccagcc atgttttata ccatgttatg gcgaatggtg atgccgacat
    14401 gctgaaaatt gttttggtcg cgttaccttt gttaattcgc acatgtcatt tgactaaaga
    14461 acaggtactc gatctcctga aggcaaagga tttttatggt tgcccaagac tatacctggc
    14521 gatgcaaaat ggacatagct atatcgtgaa agttattctc gaagcattgc cctgtctggc
    14581 ccaggaaatt aacatttcag cttccgatat tgtagatctt ctgaccgcta aaagtcttgc
    14641 gcgcgacacg ggtttgttta tggccatgca gcgcggacat atgaacgtta ttaatactat
    14701 ttttaacgca ttacccactc tttttaatac gtttaaattc gataaaaaaa tatgaagccc
    14761 ctcctcctgg caaataattc taatgaatat cccggtttgt tttcagcgat acagcataaa
    14821 caacaaaacg ttgtagagac ggtttatctt gctttatctg accatgcacg cctgtttgga
    14881 tttaccgctg aagatattat ggatttttgg caacacaaag cgccacaaaa atactctgcc
    14941 tttgagttgg cttttgaatt tggtcaccgg gttattgctg aattaatcct taatacatta
    15001 aataagatgg ctgaaagctt tggctttacg gataaccctc gatacattgc ggagaaaaat
    15061 tatatggaag ctttactcaa aaaagcatct ccccataccg tacgctaaat ctttgcctgc
    15121 gccgatacgc ataaacatct tccacatgcc cttcacgtat gcggttttgt agtgcgcgcc
    15181 agtaatcagc gcggaacagg tcggcgtgca tctcttcaaa cagcggacca atacgcgggt
    15241 cggcgcatag ccagtggcga aactcttccg ggaaaacatc gcccggcgag acgctgtacc
    15301 acggttcgct ggcaagttcg tcttccggat agcgcggcgg cgggatgtcg cggaaattca
    15361 cttccgtcat gtagcaaatt tcatcgtaat cataaaaaac cacacgcccg tgacgggtga
    15421 caccgaagtt tttaaacagc atgtcgccag ggaaaatgtt agcagcggca agctggcgaa
    15481 tagcgttacc gtattcttca atggcgtcgc gcaactgctg accttccact tgttccagcc
    15541 agatattgag cggcaccatc cgccgctcga tatagagatg gcgaattaca atctgctcac
    15601 caagatcggt gattttttcc gctgcttcct ggagcagtaa ttccattaat accggggaaa
    15661 tatgccgctt ctccagcaca aagttttcaa actcctgggt gtccgccatt cggcccacac
    15721 ggtcgtgctc tttcaccagt tgatagcagg cacgaacgtg agcggcagac atctctttct
    15781 gcggcgcgaa cttgtctttg atgactttga atacccgatc aaagcccggc agcgtaaaca
    15841 ccaacatcac cattccacga atacccggcg cttcaatgaa ctgctcatta cagccctgta
    15901 gataaacgag atattcgcgg tagctttcgg ttttggcgtg cttctggcag ccgatagcca
    15961 tatacaattc agcagtggtt ttacctggca gaatttcccg tagccactcg accagcgctg
    16021 cgggcagcgg tgcataaacc ataaaataag aacgcgcaaa gccaaaaaca atgctcgctt
    16081 cggcggtcgt cgtcaggcag gtatcaataa ataactcgcc gtcgtccgtc tggtggatcg
    16141 gcagtaagaa gggcaacgtg ccggaaggcg tgatcagttt gcccaccagc caggcggctt
    16201 tattgcggta aaacagttcg ttcgccacct gtaaatgact ttccgcgagg ttgtctgtcc
    16261 ccagcgtttc cgtcagatgg cgaatgatgt aatgaatgtc acggctttta ttctgccagc
    16321 gcaggcgcag cggcaggtcg ctgataacgc gcatcagtag agattcccag ccgtgatcgg
    16381 ggtgaaagtc tttcgccagc gggcggggaa tggtacgaaa gcggcgctct ggctgagagc
    16441 taaagataaa aagccgctcg ggagtaagcg agcggtggtc aaataaccga cagtacacgg
    16501 agttaaaaaa gctctccgca atctcgaagc gcgggtaatc cggcaacagc cgcgtgtaat
    16561 gctctttaac gcgcagtaaa aatgccgcgt ccgtactttg gccgttagta atgcagcgca
    16621 gttgctccac gaccagacca acgtgatgat cgtaaagatg gatacggttt ttcatcgcct
    16681 gctggacagc atgccagtcg gcctgttcga aacgctgctg cgccccggaa gtcacttcga
    16741 ggaatcgacc atactgagcg tcgaagcctt gcaaaatggt ttgagcaatc agtaattcca
    16801 ggccacgcgg catttagcgc actcatcagg agcagagaat tgccggatgc ggcgtaaaca
    16861 ccttatccag cctacgttcg gcaacggttg taggcatgat aagacgcgca agcgtcgcat
    16921 caggcatcgt gcaccgattg ccggatgcgg cgtaaatgcc ttatccggcc tacagtcagc
    16981 aacgtttgta ggttgcttag aactgcgatt cttcagtgga gccagtcagc gcggtgactg
    17041 aagacgtgcc gccctgaata atagtcgtca ctttatcgaa gtaacctgta cccacttcct
    17101 gctggtgaga tacgaaggta tagccatctt tcgcggcggc aaattccggc tgctgcactt
    17161 tctcaacata gtgcttcata ccttcgccct gcgcataagc gtttgccagg tcgaacatgt
    17221 taaaccacat gctgtggata cctgccaggg tgatgaactg gaacttgtag cccatatccg
    17281 acaactgctg ctggaagctg gcaatagttt tgtcgtcgag gtttttctgc cagttgaacg
    17341 acggcgagca gttataagcc agcagtttgc ccggatattt cgcgtggata gcttgtgcaa
    17401 agcgacgcgc cagttccaga tccggcgtgg aggtttcaca ccagaccagg tcagcatatg
    17461 gcgcatacgc caggccacgg ctgatcgctt gctcaatgcc cgcatgagta cggaagaagc
    17521 cttcactggt acgctcgccg gtaataaatt cgctgtcata cgggtcgcaa tctgaggtga
    17581 tcagatccgc cgcatcagca tcggtacgtg caaccagcag ggttggaacg cccgtcacgt
    17641 cagctgccag acgcgccgcg accagtttct gaatagcttc ctgagttggc actaaaactt
    17701 tgccgcccat gtgaccgcat ttcttcactg acgccagctg atcttcgaag tgaactgccg
    17761 cagcaccggc ttcaatcatc gctttcatca gttcaaaggc attcaggaca ccgccaaaac
    17821 cggcttccgc atcggcaacg atcggcagga agtaatcgac atagcgcgga tcgcccggct
    17881 caatgcccac ggaccattgg atctgatcgg cacgacggaa ggtgttgttg atccgctcca
    17941 ccacagctgg caccgagttt gccggataga gcgactgatc cggatacatg ctggccgcca
    18001 ggttagcgtc cgccgctacc tgccatcccg acagatagac tgcttcaata cccgctttcg
    18061 cctgttgcag cgcctgaccg ccagtcagtg caccgaggct gttgatgtag ccttttttcg
    18121 actcaccgtg cagcagacgc cacattttcg ctgcgcccag ttgcgccagc gtgcattcag
    18181 gattgactga accgcgtaat ttcaccacat cttccgcact gtatgggcga gtaatgcctt
    18241 cccaacgcgg ttgagtccac tctttctgta attcttcaat ttgttgtgta cgggttttca
    18301 tgtgcagatg ctccatagtt atgtggtggt ttacgctaac aggcggtagc ctggcagggt
    18361 caggaaatca attaactcat cggaagtggt gatctgttcc atcaagcgtg cggcatcgtc
    18421 aaaacggccc tgggagaaac gttcttcgcc cagttcgctg gcaatgactt tcatctcttc
    18481 gcccagcatc tggcggaaca aggctttggt caccggtttg ccattgctca acgttttttg
    18541 atgatggatc cactgccaga tcgaggtacg ggaaatttca gccgtcgccg catcttccat
    18601 caggccataa atcggcacgc agccgttgcc ggagatccac gcttcgatgt actgcacagc
    18661 cacgcgaatg ttggcgcgca taccttcttc ggtgcgttca ccatcacaag gtgccagcag
    18721 ctgatcggca gtaatcggcg cgtcttgttc gcgcaatact tcaagctgat ttttacggga
    18781 gccgagaata tcgttgaata ccgccattgc cgtgtccgca aggcctgggt gagcgatcca
    18841 tgtgccatcg tgaccgttat tggcttccag cgctttatcc gcttttactt tgttgagcac
    18901 ctggttatta cgctcttcat ctttgctcgg aataaacgct gccatgccgc ccatcgcaaa
    18961 agcaccgcgt ttatggcagg ttttaatcag caggcgtgag taagcattca ggaatggttt
    19021 atccatcgtc acggcctgtc tgtctggcag gacacgatcg ggatagtttt tcaacgtttt
    19081 gatatagctg aagatgtaat cccagcgacc gcagttcaga ccaacaatat ggtcgcgcag
    19141 cgcgtgaagg atttcgtcca tctggaacac cgcgggcagc gtttcaatca gcaacgtcgc
    19201 cttgatggtg ccgcgcggca gattaaagcg atcttctgca tagctgaaga cttcgctcca
    19261 ccaggccgct tcctgccagg actgggtttt cggcagatag aaatagggac cgcagccctt
    19321 tgccaacagt gcctgatagt tgtggaagaa atagagcgca aaatcaaaca ggctaccggg
    19381 gattgcctca ccacgccagg tgacatgttt ttccggcaag tgcagaccgc gtacccgaca
    19441 aatcaaaacc gctggattgg gcttgagctg ataaattttg cctgcttcat tggtatagct
    19501 gatggtaccg ttaaccgcat cgcgcaggtt aatttgcccg tcgatcactt tgttccagtc
    19561 tggtgccagt gaatcttcga aatcggccat aaagactttc acattggcgt tcagtgcgtt
    19621 gatcaccatc ttgcgctcta ccgggccagt tatctctacg cggcggtctt ctaagtccgc
    19681 aggaatcccg cgaattttcc aatcagcatc gcgaatggaa gccgtttccg aaataaaatc
    19741 aggcaacgtt ccgttatcaa tatcttgctg ctgctgaatg cgcgctgcca gaagtttatt
    19801 gcgttgtggc gtaaaatgcg tcaccagctc agtcagaaat tctaccgctt cggcagtaag
    19861 aatttgcttc tcctgctcgc catacggcct tgtgaaagcc agttcatcgg ttgttgttgc
    19921 ctgttcagtc atcgtgcagc tcctcgtcat ggatccgaaa acttcccctc tgtgaacgaa
    19981 ggaatgttgt gcgcttttcg ttcagcacaa ctaagactac tcatttaaaa tgcaaaaaca
    20041 aaaacaattt ccattttaat taaaaatact tgataactta ttgataacaa tatgaataaa
    20101 atttattcac ttgctgaggt gcgtttcgga aataaaaaag gcacccgaag gtgcctgagg
    20161 taaggtgctg aatcgcttaa cgatcgacta tcacagaaga ttaatccagc gttggattca
    20221 tgtgccgtag atcgtatggc gtgatctgat agacgtaata gttgagccag ttggtaaaca
    20281 gcaaattacc gtgactacgc cagctcgctc gcggtttgtt ttgcggatcg ttgtgcggga
    20341 aatagttata cggtacatcc gggtctagtc cggcttccac atcgcggaaa tattcctgcg
    20401 ccagcgtttg cgcatcatat tcgggatggc ccgtcacaaa ggcaatgcgc ttatctttgc
    20461 tggcaaacag atatgcatcc ccttcttccg tctctgccag aatttccaga tcggtgtaat
    20521 cacgaatcaa cgctgccgga aagtcagcat agcgcgaatg cggtgccagg aatgaatcat
    20581 caaagccacg cgtcagaagc gcatgaggat ggagaatatg atgctcgtaa acgccagaga
    20641 gtttgtcggt gcgagtttgc ttaggaatgc cgtagaggat attgagcgcg gcctgtaccg
    20701 cccagcagac aaacagcgtc gaggtgacgt gatctttcga ccactccagc acctgtttga
    20761 tctgcggcca gtaagcgaca tcattaaact ccaccaggcc cagcggcgca ccggttacaa
    20821 tcagaccgtc aaagttctgt tcctgaatat cttcaaagtt acagtagaag ttgttcagat
    20881 gctctgcggg cgtgttgcgc gattcacggg aatcgatgcg caacagctga atatcgacct
    20941 gcaaaggtga gtttgaaagc aggcgcagaa actgattttc agtttcaatc ttcttcggca
    21001 tcaggttaag gatcagaacc ttaagtggac gaatttcctg accagacgca cgagaagttg
    21061 tcatcacaaa gacgttttct tcacgcaaga aattgacggc gggtagctcg tccggcacac
    21121 gaatcggcat aacctgatta cctcactaca tacgcttata cgtttagaca tccagatagc
    21181 tgaagataac cagaaaattt gccaatgtcg agcctgcatg ttgaaggtga gaaagtttca
    21241 aggaaaaggc tacgttagaa tataagaatg acgaaaagga gagaaaatgg ttattagtat
    21301 tcgccgctca cggcatgagg aaggggagga actcgttgcg atttggtgtc gatctgtcga
    21361 tgccacccac gattttttat cagcagagta tcgggccgag ctggaagagc tggtgcgttc
    21421 ctttctgccg gaagcaccgt tgtgggtcgc ggttaatgaa cgggagcagg cggttgggtt
    21481 ctataagaag gtgggtttta aggttacggg acgctctgag gtggacgatt tggggaaacc
    21541 gtatccgttg ctgaatctgg cgtatgtggg ggaataagcc gcccctgccg tgcctgccca
    21601 agctggagaa gacgcgccag cgtcgcaccc ggcatttttt tcac
//
LOCUS       DAETPZ010000062        21504 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.062, whole genome shotgun sequence.
ACCESSION   DAETPZ010000062 DAETPZ010000000
VERSION     DAETPZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.062"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="K0W59_002690"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="K0W59_002690"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBI3023669.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="K0W59_002691"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="K0W59_002691"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBI3023670.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEMEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="K0W59_002692"
     CDS             complement(3121..3420)
                     /locus_tag="K0W59_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HBI3023671.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /gene="wecH"
                     /locus_tag="K0W59_002693"
     CDS             3595..4590
                     /gene="wecH"
                     /locus_tag="K0W59_002693"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HBI3023672.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /locus_tag="K0W59_002694"
     CDS             complement(4632..5069)
                     /locus_tag="K0W59_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HBI3023673.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /locus_tag="K0W59_002695"
     CDS             complement(5115..5456)
                     /locus_tag="K0W59_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023674.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5726..7078)
                     /gene="xylB"
                     /locus_tag="K0W59_002696"
     CDS             complement(5726..7078)
                     /gene="xylB"
                     /locus_tag="K0W59_002696"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBI3023675.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMLALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGMWGQHWAQQGWRRLRRIQRNRSLSCCRSYR
                     "
     gene            complement(7150..8472)
                     /gene="xylA"
                     /locus_tag="K0W59_002697"
                     /pseudo
     CDS             complement(7150..8472)
                     /gene="xylA"
                     /locus_tag="K0W59_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8838..9830
                     /gene="xylF"
                     /locus_tag="K0W59_002698"
     CDS             8838..9830
                     /gene="xylF"
                     /locus_tag="K0W59_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI3023676.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     TKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKEKEL"
     gene            9908..11449
                     /gene="xylG"
                     /locus_tag="K0W59_002699"
     CDS             9908..11449
                     /gene="xylG"
                     /locus_tag="K0W59_002699"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HBI3023677.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11427..12608
                     /gene="xylH"
                     /locus_tag="K0W59_002700"
     CDS             11427..12608
                     /gene="xylH"
                     /locus_tag="K0W59_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HBI3023678.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12686..13216
                     /locus_tag="K0W59_002701"
     CDS             12686..13216
                     /locus_tag="K0W59_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBI3023679.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13328..13864
                     /locus_tag="K0W59_002702"
     CDS             13328..13864
                     /locus_tag="K0W59_002702"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBI3023680.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13972..14796)
                     /locus_tag="K0W59_002703"
     CDS             complement(13972..14796)
                     /locus_tag="K0W59_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HBI3023681.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15116..17146
                     /gene="malS"
                     /locus_tag="K0W59_002704"
                     /pseudo
     CDS             15116..17146
                     /gene="malS"
                     /locus_tag="K0W59_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            17324..18577
                     /gene="avtA"
                     /locus_tag="K0W59_002705"
     CDS             17324..18577
                     /gene="avtA"
                     /locus_tag="K0W59_002705"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HBI3023682.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRSLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18728..19201)
                     /locus_tag="K0W59_002706"
     CDS             complement(18728..19201)
                     /locus_tag="K0W59_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBI3023683.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="K0W59_002707"
     CDS             complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="K0W59_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HBI3023684.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTHNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGALT"
     gene            20351..21349
                     /gene="yiaK"
                     /locus_tag="K0W59_002708"
     CDS             20351..21349
                     /gene="yiaK"
                     /locus_tag="K0W59_002708"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HBI3023685.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21361..>21504
                     /locus_tag="K0W59_002709"
     CDS             21361..>21504
                     /locus_tag="K0W59_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBI3023686.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQ"
BASE COUNT         5406 a         5488 c         5277 g         5333 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccatttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaacctt tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 cccccgtacg gtaatcgagc tgctgaccgc tgatatccgc cagcatctga cgccagtact
     5881 cactacgcgc cccgccacca atcaacgtaa cactttgcgg tttaatgccg caggcatgca
     5941 cgacatccat gccatctgcc agcgcataac ccacgccttc cagcactgct cgcgccagtt
     6001 cattggggcc atgttgatga gtcaaaccaa agaaaacccc cttcgcctgg ggattattgt
     6061 gtggtgtacg ctcgccggaa agataaggca gaaaccaaac tggctcggca ctttcatcag
     6121 cctgttgagc tgcagcgatt aaagctggga cattgcacag gccggttaat ttcgcggccc
     6181 aatccagaca cgatgctgca ctcagcatta cagacattaa atgccaacgc tgcggtagcg
     6241 catggcaaaa gctgtgtaca gcgctttctg gcttgcttaa gaacccttcg ctgacagcaa
     6301 aatagacccc cgacgtcccc agcgataaca ttgcctgatt agcatcaacc attcccacac
     6361 caactgcacc agctgcattg tcgccaccgc ctgcgacaac tggcaccgtc gccatacccc
     6421 acgctttcgc aacttcaggt aacaaagcac cagtaatttc gctgccttcg tataatgcga
     6481 gcatctggtc acgagataag tcgcaagcct gcagcatgac gtcactccag tcacgctttg
     6541 cgacatccag ccacatggtg ccagctgcgt cagacatatc gctggcaaac tcccccgtca
     6601 tacgcagacg caagtaatct ttcggtaata atactttgtc gatttgacgg aatatctccg
     6661 gctcatgccg ctgaacccat agcaatttag gcgcagtaaa tccgggcatc atcaggttgc
     6721 cggtaatcac tcgtgattgc ggaactctcg cttccagcaa agtgcactct tgcgcacagc
     6781 gcccgtcgtt ccacaaaatg gcagggcgca atacccgttg ttgagcatcc agtaaggttg
     6841 ctccatgcat ctggccggca atacccaatg ctttaacgtc ctgcagagaa tgctgatcgc
     6901 ccagagcttt cattgcgcga tcagttgcct gccaccactg ttccgggtct tgttccgacc
     6961 aaagtggatg cgggcgcgaa acggtcagct tttccgtttg cgaagcaacc acctcaccct
     7021 gctcgttgag caaaataact tttacgcccg aggtgccaag atctatcccg atatacatat
     7081 cgatcgttcc ttaaaaaaat gcccggtatc gctaccgata accgggccaa cggactgcac
     7141 agttagccgt tatttgtcga acagataatg atttaccaga ttttccagtt gctcctggcg
     7201 accactctga tgcaccggag acaaattatg ttcctgagca tatttggcta aatctgccag
     7261 tgacatttgg cctttcagga tttgctggcc caattcgcta ttccagccgg aataacgctg
     7321 cgcgatgcgt ttatccagct cgccatcttc aatcatgcgc gctgcaattt tcagcgccag
     7381 tgccatcgta tccatcgcgc cgatatgacc gtaaaacaga tcatatttat cagtactttg
     7441 acgacgtact ttggcatcga agttcagacc accggtggtg aaaccgcctg ctttgagaat
     7501 ttcatacatc accagcgcat tctcttccac actgttcggg aactggtcgg tgtcccagcc
     7561 cagttgcgca tcgccacggt tggcgtcgac agaaccgaac aggccaagcg caatggcggt
     7621 ggctatttca tgatggaaag agtgacctgc cagcgtcgcg tggttagctt caatgttcag
     7681 tttaatctct ttttccagac caaactgttt caggaagcca tagaccgtcg cggcatcgta
     7741 atcatattga tgtttagtcg gttcttgcgg tttcggttcg ataagcaacg tgccctagaa
     7801 gccgatttta tgtttatgct caaccaccat ctgcataaag cggcccagtt gttcacgctc
     7861 ctgacgcaag tcggtattta acagcgtttc gtaaccttca cgaccacccc acaggacata
     7921 gttttcaccg cccaatttat gggttgcttc catcgctgta acaacttgcg ttgccgccca
     7981 gctgaagact tcaggatctg ggttcgtcgc cgcacccgcg ccatagcgag ggtttgtaaa
     8041 gcagttagcg gttccccaca gcagcttcac gccgctctct tcttgcttgg ctgccaggac
     8101 atcaaccatt tgcgcaaaat tattgatgta ctcttttaac gacgcgccct caggggaaac
     8161 atccacatcg tggaagcaat aaaatggcac atgtaacttg tggaaaaact caaatgcgac
     8221 atctgcttta cgcttcgcca acgccagtgc ctcaccaggc tgctgccacg gacgattaaa
     8281 cgcccccaca ccaaacatgt ccgccccgtt ccagcagaag gtgtgccagt agcaggcggc
     8341 aaaacgcaag tgctcttcca tacgcttacc caacaccagt tcgtcgggat tgtagtgacg
     8401 gaatgctaac gggtttgagg attttgagcc ttcataacga acgcgatcga gctggtcaaa
     8461 ataggcttgc atattgaact ccataatcag gtaatgccgc gggtgatgga tgatgtcgta
     8521 atattgggca ctccctttca attgctcaat tatgttattt cacactgcta ttgagataat
     8581 tcacaagtgt gcgctcgctc gcaaaataaa atgggatgat gaaactgggt aattcctcga
     8641 agataaaaat gcaataagta caattgcgca acaaaagtaa gatctcggtc ataaatcaag
     8701 aaataaacca aaaatcgtaa tcgaaagata aaaatctgta attgttttcc cctgtttagt
     8761 tgctaaaaat tggttacgtt tatcgcggtg attgttactt attaaagctg tcctctaact
     8821 acagaaggcc ctacaccatg aaaataaaga acattctact caccctttgc acctcacttc
     8881 tgcttaccaa cgttgcggca cacgccaaag aagtcaaaat aggtatggcg attgatgatc
     8941 tccgtcttga acgctggcaa aaagatcgag atatttttgt gaaaaaggca gaatctctcg
     9001 gtgcgaaagt atttgtacag tctgcaaatg gcaatgaaga aacacaaatg tcgcagattg
     9061 aaaacatgat taaccggggt gtcgatgttc ttgtcattat tccgtataac ggtcaggtat
     9121 taagtaacgt tgtaaaagaa gccaaacaag aaggtattaa agtattagct tacgaccgta
     9181 tgattaacga tgcggatatc gatttttata tttctttcga taacgaaaaa gtcggtgaac
     9241 tgcaggcaaa agccttggtc gatattgttc cgcaaggtaa ttacttcctg atgggcggct
     9301 cgccggtaga taacaacacc aagctgttcc gcgccggaca aatgaaagtg ttaaaacctt
     9361 acgttgattc tggaaaaatt aaagtcgttg gtgaccaatg ggttgatggc tggttaccgg
     9421 aaaacgcatt gaaaattatg gaaaacgcgc taaccgccaa taataacaaa attgatgctg
     9481 tagttgcctc aaacgatgcc accgcaggtg gggcaattca ggcattaagc gcgcaaggtt
     9541 tatcagggaa agtagcaatt tccggccagg atgcggatct cgcaggtatt aaacgtattg
     9601 ctgccggtac gcaaactatg acggtgtata aacctattac attgctggca aatactgccg
     9661 cagaaattgc cgttgaattg ggcaatggtc aggagccaaa agcagatacc tcactgaata
     9721 atggcctgaa agatgtcccc tcccgcctcc tgacaccgat cgatgtgaat aaaaacaaca
     9781 tcaaagatac ggtaattaaa gacggattcc acaaagagaa agagctgtaa gcgttacgcc
     9841 ccggcgcgga gcgggggcgt gatttctctc catgccgcgt gaatgaattg gcttaggtgg
     9901 agtcgttatg ccttatctac ttgaaatgaa gaacattacc aaaaccttcg gcagtgtgaa
     9961 ggcgattgat aacgtcagct tgcggttgaa tgctggcgaa atcgtctcac tctgtgggga
    10021 aaatgggtct ggtaaatcaa cgctgatgaa agtgctgtgt ggtatttatc cccatggctc
    10081 ctacgaaggc gaaattattt ttgcgggaga agagattcag gcgagtcaca tccgcgatac
    10141 cgaacgcaaa ggtatcgcca tcattcacca ggaattggcc ctggtgaaag aattgaccgt
    10201 gctggaaaat atcttcctgg gtaacgaaat aacccacaat ggcattatgg attatgacct
    10261 gatgacgcta cgctatcaga agctgctcgc acaggtcagt ttatccattt cacctgatac
    10321 ccgcgttggc gatttagggc ttgggcaaca acaactggtt gaaattgcca aggcacttaa
    10381 taaacaggtg cgcttgttaa ttctcgatga accgacagcc tcattaactg agcaggaaac
    10441 gtcggtttta ctggatatta ttcgcgatct acaacagcac ggtatcgcct gtatttatat
    10501 ttcgcacaaa ctcaacgaag tcaaagcgat ttccgatacg atttgcgtta ttcgcgacgg
    10561 acagcacatt ggtacgcgtg atgctgccgg aatgagtgaa gacgatatta tcaccatgat
    10621 ggtcgggcga gagttaaccg cgctttaccc taatgaacca cataccaccg gagatgaaat
    10681 attacgtatt gaacatctga cggcatggca tccggtcaat cgtcatatta aacgagttaa
    10741 tgatgtctcg ttttccctga aacgtggcga aatactgggt attgccggac tcgttggtgc
    10801 cggacgtacc gagaccattc agtgcctgtt tggcgtgtgg cccggacaat gggaaggaaa
    10861 aatttatatt gatggcaaac aggtagatat tcgtaactgt cagcaagcca tcgcccaggg
    10921 gattgcgatg gtacccgaag acagaaagcg cgacggcatc gttccggtaa tggcggttgg
    10981 taaaaatatt accctcgccg cactcaataa atttaccggt ggcattagcc agcttgatga
    11041 cgcggcagag caaaaatgta ttctggaatc aattcagcaa ctcaaagtta aaacgtcgtc
    11101 ccccgacctt gctattggac gtttgagcgg cggcaatcag caaaaagcga tcctcgcccg
    11161 ctgtctgtta cttaacccgc gcatcctcat tcttgatgaa cctaccaggg gtattgatat
    11221 tggcgcgaaa tatgagatct acaaattaat taaccaactc gtccagcagg gtattgccgt
    11281 tattgtcatc tcttccgaat tacctgaagt gctcggcctt agcgatcgtg tactggtgat
    11341 gcatgaaggg aaactaaaag ccaacctgat aaatcataac ctgactcagg agcaggtgat
    11401 ggaagccgca ttgaggagcg aacatcatgt cgaaaagcaa tccgtctgaa gtgaaattgg
    11461 ccgtaccgac atccggtggc ttttctgggc tgaaatcact gaatttgcag gtcttcgtga
    11521 tgattgcagc tatcatcgca atcatgctgt tctttacctg gaccaccgat ggtgcctact
    11581 taagcgcccg taacgtctcc aacctgttac gccagaccgc gattaccggc atcctcgcgg
    11641 taggaatggt gttcgtcata atttctgctg aaatcgacct ttccgtcggc tcaatgatgg
    11701 ggctattagg tggcgtcgcg gcgatttgtg acgtctggtt aggctggcct ttgccactta
    11761 ccatcattgt gacgttggtt ctgggactgc ttctcggtgc ctggaacgga tggtgggtcg
    11821 cgtaccgcaa agtcccttca tttattgtca ccctcgcggg catgttggca tttcgcggca
    11881 tactcattgg catcaccaac ggcacgactg tatcccccac cagcgccgcg atgtcacaaa
    11941 ttgggcaaag ctatctcccc gccagtaccg gcttcatcat tggcgcgctt ggcttaatgg
    12001 cttttgttgg ttggcaatgg cgcggaagaa tgcgccgtca ggctttgggt ttacagtctc
    12061 cggcctctac cgcagtagtc ggtcgccaag ctttaaccgc tatcatcgta ttaggcgcaa
    12121 tctggctgtt gaatgattac cgtggcgttc ccactcctgt tctgctgctg acgttgctgt
    12181 tactcggcgg aatgtttatg gcaacgcgga cggcatttgg acgacgcatt tatgccatcg
    12241 gcggcaatct ggaagcagca cgtctctccg ggattaacgt tgaacgcacc aaacttgccg
    12301 tgttcgcgat taacggatta atggtagcca tcgccggatt aatccttagt tctcgacttg
    12361 gcgctggttc accttctgcg ggaaatatcg ccgaactgga cgcaattgca gcatgcgtga
    12421 ttggcggcac cagcctggct ggcggtgtgg gaagcgttgc cggagcagta atgggggcat
    12481 ttatcatggc ttcactggat aacggcatga gtatgatgga tgtaccgacc ttctggcagt
    12541 atatcgttaa aggtgcgatt ctgttgctgg cagtatggat ggactccgca accaaacgcc
    12601 gttcttgatt ttgataaaaa ttttctcaaa gccggttacg tattaccggt tttgagtttt
    12661 tgcatgattc agcaggaaaa gaaccatgtt tactaaacgt caccgcatca cattactgtt
    12721 caatgccaat aaagcctatg accggcaggt agtagaaggc gtaggggaat atttacaggc
    12781 atcacaatca gaatgggata ttttcattga agaagatttc cgcgcccgca ttgataaaat
    12841 caaggactgg ttaggagatg tcgtcattgc cgacttcgac gacaaacaga tcgagcaagc
    12901 gctggctgat gtcgacgtcc ccattgttgg ggttggcggc tcgtatcacc ttgcagaaag
    12961 ttacccaccc gttcattaca ttgccaccga taactatgcg ctggttgaaa gcgcattttt
    13021 gcatttaaaa gagaaaggcg ttaaccgctt tgctttttat ggtcttccgg aatcaagcgg
    13081 caaacgttgg gccactgaac gcgaatatgc atttcgtcag cttgtcgccg aagaaaagta
    13141 tcgcggagtg gtttatcagg ggttagaaac cgcgccagag aactggcaac acgcgcaaaa
    13201 tcggctggca gactgactac aaacgctgcc accgcaaacc gggattattg ccgttactga
    13261 cgcccgagcg cggcatattc tgcaagtatg tgaacatcta catattcccg taccggaaaa
    13321 attatgcgtg attggcatcg ataacgaaga actgacccgc tatctgtcgc gtgtcgccct
    13381 ttcttcggtc gctcagggcg cgcggcaaat gggctatcag gcggcaaaac tgttgcatcg
    13441 attattagat aaagaagaaa tgccgctaca gcgaattttg gtcccaccag ttcgcgtcat
    13501 tgaacggcgc tcaacagatt atcgctcgct gaccgatccc gccgttattc aggccatgca
    13561 ttacattcgt aatcacgcct gtaaagggat taaagtggat caggtactgg atgcggtcgg
    13621 gatctcgcgc tccaatcttg agaagcgttt taaagaagag gtgggtgaaa ccatccatgc
    13681 aatgattcat gccgagaagc tggagaaagc gcgcagtctg ctgatttcaa ccaccttgtc
    13741 gatcaatgag atatcgcaaa tgtgcggtta tccatcgctg caatatttct actctgtttt
    13801 taaaaaagca tatgacacaa cgccaaaaga gtatcgcgat gtaaatagcg aggtcatgtt
    13861 gtagcgatga tgagaattgt cggcgtcaca tcagggaact cagcacagcc acctgatgcg
    13921 agcaaaagcc ttatccgacc gacaaatcag ataaggctcg aggaaatgaa attacatatg
    13981 cgccgcgatt aaccgttggt tatcctggta cattgcgaac aggtagttgt tataactctt
    14041 ccctttggtc gagtagccct tcagcttgtg aatcatcgct gtggcagtca cttcctgatc
    14101 cgctttacgc agctgtgcac gcgatttacg gaacgaagag taagccgggt gcgtattcag
    14161 gttagtgaca taggcgctca ccgattcttt gacagaacta aactgtgagt accctttcac
    14221 tttacccggc gcattggtac aacgtccttt catgcatttc atgccgaaca gattgttatt
    14281 gttgcgcgcc agcttcgacg ttccccaacc gctttctgct gcagccatcg tcgccaccat
    14341 actggtaggg ataatgtcta cgcgttcaag caaggtattc cacgggattt ttcgcgtatt
    14401 accagaccac ttcaccttgt agcgtttggc gatgtctttc agacgcgcac gctcagcagg
    14461 tgaccattgg ccctgatact gttttgaaat cagccagtta cgttctgcag taatggctgc
    14521 attttggctg gtaatgtaag gcattacggt ccggagaaac gcctttttcc ttggtgttcc
    14581 ggaagggtat tttcgcaaat caggaagtga actactcttt gcactattgc gagaatactc
    14641 ttgtttactg cttacctgtt tattactagc tttagttaag tgggactttt gactcgctgt
    14701 tgttgtgtgc gtcttcgcta acacctcact cgaaaacacc agagtgagta acataagaat
    14761 catcgcccca tatcgtcgta tgggagtcaa aatcatcagg tctcctggtc ggatttaatc
    14821 attccaacac cttatatttt tcacaaattt gagagttgaa tctcaaatca tatcaaaaat
    14881 agctgtcaag agcaccccaa ggaatagtcc aaatctgaaa ctatgtcacg agttaacgat
    14941 tcagattggc gctaaatcgc agaaaatgtg ggggttatcg caaaattcag ccgttttttg
    15001 cgcgcgatcg ctcacccttg cttctcatcc tgtggactta ccgctcaggg atgagttttg
    15061 tctggcttat cgctggcaaa ctgtctgaaa tcgcagcaat aaggactcat ccaccatgaa
    15121 actcgccgcc tgttttctga cactccttcc tggcttcgcc gttgccgcca gctggacttc
    15181 tccggggttc cctgccttta gcgaacaggg aacgggaaca tttgtcagcc acgcgcagtt
    15241 gcccaaaggt acgcgtccac tcacgctaaa ttttgaccaa cagtgctggc agcctgcaga
    15301 tgcgataaaa ctcaatcaga tgctttccct gcaaccttgt agcaacacgc cgcctcaatg
    15361 gcgattgttc agggacggca aatatacgct gcaaatagac acccgctccg gtacgccaac
    15421 attgatgatt tccatccaga acgccgccga accggtagca aacctggtcc gtgaatgccc
    15481 gaaatgggat ggattaccgc tcacgctgga tgtcagcgcc actttcccgg aaggagccgc
    15541 cgtacgggat tattacagcc aacaaattgc gatagtgaag aacggtcaaa taacgttaca
    15601 acccgctgct accagcaacg gtttactcct gctggaacgg gcagaaactg acacatccgc
    15661 ccctttcgac tggcataacg ccacggttta ctttgtgctg acagatcgtt tcgaaaacgg
    15721 cgatcccagt aatgaccaga gttacggacg tcataaagac ggtatggcgg aaattggcac
    15781 ttttcacggc ggcgatttac gcggcctgac caacaaactg gattacctcc agcagttggg
    15841 cgttaatgct ttatggataa gcgccccatt tgagcaaatt cacggctggg tcggcggcgg
    15901 tacaaaaggc gatttcccgc attatgccta ccacggttat tacacacagg actggacgaa
    15961 tcttgatgcc aatatgggca acgaagccga tctacggacg ctggttgata gcgcacatca
    16021 gcgcggtatt cgtattctct ttgatgtcgt gatgaaccac accagctatg ccacgctggc
    16081 ggatatgcag gagtatcagt ttggcgcgtt atatctttct ggtgacgaag tgaaaaaaac
    16141 gctgggtgaa cgctggagcg actggaaacc tgccgccggg caaacctggc atagctttaa
    16201 cgattacatt aatttcagcg acaaaacagg ctgggataaa tggtggggaa aaaactggat
    16261 cagaacggat atcggcgatt acgacaatcc tggattcgac gatctcacta tgtcgctggc
    16321 ctttttgccg gatatcaaaa ccgaatcaac taccgcttct ggtctgccgg tgttctataa
    16381 aaacaaaacg gatactcacg ctaaagtcat cgaaggcttt acacctcgcg attacttaac
    16441 ccactggtta agtcagtggg tccgcgacta tgggattgat ggttttcggg tcgataccgc
    16501 caaacatgtt gagttgcccg cctggcagca actgaaaacc gaagccagct ccgcgcttcg
    16561 cgaatggaaa aaaactaacc ccgacaaagc attagatgac aaacctttct ggatgaccgg
    16621 tgaagcctag ggccacggcg tgatgcaaag tgactactat cgccacggct tcgatgcgat
    16681 gatcaatttc gattatcagg agcaggcggc gaaagcagtc gattgtctgg cgcagatgga
    16741 tacgacctgg cagcaaatgg cggagaaatt gcagggtttc aacgtgttga gctacctctc
    16801 gtcgcatgat acccgtctgt tccgtgaagg gggcgacaaa gcagcagagt tattactatt
    16861 agcgccaggc gcggtacaaa tcttttatgg cgatgaatcc tcgcgtccgt tcggtcctac
    16921 aggttctgat ccgctgcaag gtacacgttc ggatatgaac tggcaggatg ttagcggtaa
    16981 atctgccgcc aacgtcgcgc actggcagaa aatcagccag ttccgcgccc gccatcccgc
    17041 aattggcgcg ggcaaacaaa cgacactttc gctgaagcag ggctacggct ttgttcgtga
    17101 gcatggcgac gataaagtgc tggtcatctg ggctgggcaa cagtgacttt tccggcttcc
    17161 cgttcgtcag tacctcggga agccgccaac caggataaaa tgtcagccct aatcagcgtt
    17221 gcgggataaa gcaccgctca ctcttcaaca gaccgatttg caccccagca aatgtagcgt
    17281 tattgttacc ttccttgcta cagagttcga cagatatccc gctatgacat tctccctttt
    17341 tggtgacaaa tttacccgcc actccggcat tacgctgctg atggaagatc tgaacgacgg
    17401 tttacgcacg cctggcgcga ttatgctcgg cggcggtaat ccggcgcaga tcccggaaat
    17461 gcaggactac ttccagacgc tactgaccga catgctggaa agtggcaaag cgactgatgc
    17521 actgtgtaac tacgacggtc cacaggggaa aacggagcta ctcacactgc ttgccggaat
    17581 gctgcgcgag aagttgggtt gggatatcga accacagaat attgcactaa caaacggcag
    17641 ccagagcgcg tttttctact tatttaacct gtttgccgga cgccgtgccg atggtcgggt
    17701 caaaaaagtg ctgttcccgc tggcaccgga atacattggc tacgctgacg ccggactgga
    17761 agaagatctg tttgtctctg cgcgtccgaa tattgaactg ctgccggaag gccagtttaa
    17821 ataccacgtc gattttgagc atctgcatat tggcgaagaa accgggatga tttgcgtctc
    17881 ccggccgacg aatccaacag gcaatgtgat tactgacgaa gagttgctga agcttgacgc
    17941 gctggcgaat caacacggca ttccgctggt gattgataac gcttatggcg tcccgttccc
    18001 gggtatcatc ttcagtgaag cgcgcccgct atggaatccg aatatcgtac tgtgcatgag
    18061 tctttccaag ctggggctgc ctggctcccg ctgcggcatt atcatcgcca atgaaaaaat
    18121 catcactgcc atcaccaata tgaacggcat tatcagcctg gcacctggcg gtattggtcc
    18181 ggcgatgatg tgtgaaatga ttaagcgtaa cgatctgctg cgcctgtctg aaacagtcat
    18241 caaaccgttt tactaccagc gtgttcagga aactatcgcc atcattcgcc gctatttacc
    18301 ggaaaatcgc agcctgattc ataaaccgga aggagccatt ttcctctggc tatggtttaa
    18361 ggatttgccc attacgaccg agcagctcta tcagcgcctg aaagcacgcg gcgtgctgat
    18421 ggtgccgggg cacaacttct tcccgggact ggataaaccg tggccgcata cgcatcaatg
    18481 tatgcgcatg aactacgtac cagagccgga gaaaattgag gcgggggtga agattctggc
    18541 ggaagagata gaaagagctt gggcagaaag tcactaaaaa ttgcctgatg cgctacgctt
    18601 atcaggccta catgatttct gcaatatatt gaatttggaa gaatttgtag gccggataag
    18661 gcgtttacgc cgcatctggc ataaacaaag cgcactattc cggcgttgag aaacgccgga
    18721 aaacgtttca aacaggctgc tgctgtagcc gatgccgctg cactttctcg acatccatgc
    18781 actgcaacgc ctttgtcggg caggcttcaa cacaggccgg ccccgtctcc cgatgccagc
    18841 acagatcgca cttaatcgcc ctcgcctttt tgcgcgaaga aacgacctcc atcgcaccaa
    18901 acgggcaagc cagcatacag cttttacagc caatgcaacg tgtttgttca acgaaaatat
    18961 gcccatgttc gcggcttatc gcgtcaacag ggcagacatt cgcgcacggt gcatcttcac
    19021 actgatgaca ggctactggc gtggtccagc tgtggtcttt aatgacacga atacgggaaa
    19081 taaactcgtc tggtgacaac gcagcgcaat cctgattctc ctgatgcgac actgcgcaag
    19141 ccacttcaca ggtacggcaa ccgatacatt tcgtcgcatc cgcaataata aaccggttca
    19201 tgatcattcc tttgcactaa aacagcagag tgccagagag cgcgtcccta ctgctttgat
    19261 ccaacgaagg aaatgttaaa gttttggcac ttgtccaaag cgttatgtga gcgcgcccag
    19321 gtcatccctg acagtaaatc ccagttcatt agaaatagcc tgcgcggttt cacgcagtgg
    19381 tttcaggaga tttttctctc ccacctgttt cagacgtgat gtcgaaagcg aaatcgacac
    19441 ggcgtacggc acccgcccat gaatatcaaa caccggaaca gcaatacagg agacgccgag
    19501 ttcgttttct tctctgtcca tcgccgctcc gctttcacga atgtgcgcca gttcgtcgaa
    19561 catcgcgggc agctcggtaa tggtattgtg ggttaacggc tggatctcat gctgatggtt
    19621 ttcccagtat gacttcacgt agtccgggtg accaaacgcc atgtagatct tgcccattgc
    19681 ggaacagtag agcggcatat gctggccaat ataggcacgg gttcgcagca tcccggttgt
    19741 gggttccagc ttataaatca aaatagcgtg atcgtcttcg cggctggaga agttaatggt
    19801 ttcaccagtg gcgatgttca gtgcctcaag atgcggagcg gcgatatgaa tgatattcag
    19861 cgaagacagc gccttctgcc cgacggcaat aaatttggta gtcaggcggt aactccctgc
    19921 ggcgggcgcg gtggtcacat agccgcagga ctgtaatccc tgcaataagc gatggacggt
    19981 actcttattt aaaccagcca gctccgaaag atgcgccaac ggacaaccgt ttggatagtt
    20041 gctcaaaatc tcaatcagca tcaacccgcg aaacagactc tggcttccgg ctggacgctc
    20101 tttttcctgc gccatctcgt tctctttttt tcccatcact tctttcccca ttttgtcgcg
    20161 tcctgatggt agcgcaaagt gtgccgtagt tcacgatctc gacagataat ttataaccac
    20221 ttgattttta tgtcttttga aattcatcaa tcagattgct ttgttaaaaa gtgatcgata
    20281 tatttgaaat ctagtttcgc atattgaaat tttaagccaa aaaagcgatc aaaaaacaag
    20341 gaagcctagg atgaaagtga catttgagca gttaaaagca gcctttaatc gggtcttaat
    20401 ttcacgcggc gttgacagcg aaacggctga cgcctgtgca gagatgttcg cccgcaccac
    20461 cgaatccggc gtttattctc acggcgttaa tcgtttccct cgtttcattc aacaactgga
    20521 aaacggcgat atcattcctg atgcccaacc caaacgtata accagcctcg gcgcaattga
    20581 acagtgggat gcccagcgtt cgatcggcaa cctgacggcg aaaaagatga tggatcgcgc
    20641 cattgaactg gctgccgatc acggtattgg tctggtggca cttcgtaatg ccaaccactg
    20701 gatgcgcggc ggcagctacg gctggcaggc ggcggaaaaa ggctatattg gcatttgctg
    20761 gaccaactcc atcgccgtaa tgccgccgtg gggcgcaaaa gagtgtcgca tcggcactaa
    20821 cccgctgatc gtcgccattc cttccacgcc gatcaccatg gtcgatatgt cgatgtcgat
    20881 gttctcttac ggcatgttag aagttaaccg cctggcaggc cgtcagctcc cggtcgatgg
    20941 tggctttgat gatgagggca atttgaccaa agaacctggc gttatcgaga agaatcgccg
    21001 cattttgccg atgggctact ggaaaggttc tggcatgtcg attgtgctgg atatgatcgc
    21061 tactctcctt tccgacggcg catcggttgc cgaagtcacc caggacaaca gcgacgaata
    21121 cggcatttca caaattttta ttgccattga agtggacaag cttatcgacg gtcccacccg
    21181 cgatgccaag ctgcaacgta tcatggatta cgttactacc gctgaacgcg ctgacgaaaa
    21241 ccaggccatc cgcttacccg gccacgaatt tactaccctg ctggccgaaa accgccgtaa
    21301 cggcatcact gttgatgaca gcgtgtgggc caaaatccag gcgttatgag gagataaatc
    21361 atgatttttg gacatatcgc gcagcccaat ccgtgccgtt tgcccgccgc cattgaaaag
    21421 gcgctcgatt ttctgcgcgc taccaacttc aacgtcctgg agccgggcgt tgtcgaaatc
    21481 gacggcaaga atatttatgc gcaa
//
LOCUS       DAETPZ010000063        21359 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.063, whole genome shotgun sequence.
ACCESSION   DAETPZ010000063 DAETPZ010000000
VERSION     DAETPZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.063"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..76
                     /locus_tag="K0W59_002710"
     CDS             <1..76
                     /locus_tag="K0W59_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3023687.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            92..1018
                     /locus_tag="K0W59_002711"
                     /pseudo
     CDS             92..1018
                     /locus_tag="K0W59_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin"
     gene            1257..1316
                     /locus_tag="K0W59_002712"
     CDS             1257..1316
                     /locus_tag="K0W59_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBI3023688.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1633..1692
                     /gene="ibsE"
                     /locus_tag="K0W59_002713"
     CDS             1633..1692
                     /gene="ibsE"
                     /locus_tag="K0W59_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HBI3023689.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1812..3245)
                     /gene="hldE"
                     /locus_tag="K0W59_002714"
     CDS             complement(1812..3245)
                     /gene="hldE"
                     /locus_tag="K0W59_002714"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HBI3023690.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3293..6133)
                     /gene="glnE"
                     /locus_tag="K0W59_002715"
     CDS             complement(3293..6133)
                     /gene="glnE"
                     /locus_tag="K0W59_002715"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HBI3023691.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAAAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6156..7457)
                     /gene="ygiF"
                     /locus_tag="K0W59_002716"
     CDS             complement(6156..7457)
                     /gene="ygiF"
                     /locus_tag="K0W59_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HBI3023692.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQPLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWLYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLIQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7699..8319
                     /locus_tag="K0W59_002717"
     CDS             7699..8319
                     /locus_tag="K0W59_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBI3023693.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPNRKRKDRWMN"
     gene            8383..9621
                     /gene="cca"
                     /locus_tag="K0W59_002718"
     CDS             8383..9621
                     /gene="cca"
                     /locus_tag="K0W59_002718"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HBI3023694.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9784..10605)
                     /gene="bacA"
                     /locus_tag="K0W59_002719"
     CDS             complement(9784..10605)
                     /gene="bacA"
                     /locus_tag="K0W59_002719"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBI3023695.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10696..11064)
                     /gene="folB"
                     /locus_tag="K0W59_002720"
     CDS             complement(10696..11064)
                     /gene="folB"
                     /locus_tag="K0W59_002720"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HBI3023696.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11169..11786
                     /gene="plsY"
                     /locus_tag="K0W59_002721"
     CDS             11169..11786
                     /gene="plsY"
                     /locus_tag="K0W59_002721"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBI3023697.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11799..12730)
                     /gene="ttdR"
                     /locus_tag="K0W59_002722"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(11799..12730)
                     /gene="ttdR"
                     /locus_tag="K0W59_002722"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            12937..13780
                     /gene="ttdA"
                     /locus_tag="K0W59_002723"
                     /pseudo
     CDS             12937..13780
                     /gene="ttdA"
                     /locus_tag="K0W59_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            13777..14382
                     /gene="ttdB"
                     /locus_tag="K0W59_002724"
     CDS             13777..14382
                     /gene="ttdB"
                     /locus_tag="K0W59_002724"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HBI3023698.1"
                     /translation="MKKILTTSIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14430..15893
                     /gene="ttdT"
                     /locus_tag="K0W59_002725"
     CDS             14430..15893
                     /gene="ttdT"
                     /locus_tag="K0W59_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HBI3023699.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLLLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYPPTMVMVTLIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(15936..16949)
                     /gene="tsaD"
                     /locus_tag="K0W59_002726"
     CDS             complement(15936..16949)
                     /gene="tsaD"
                     /locus_tag="K0W59_002726"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBI3023700.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNSTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17187..17402
                     /gene="rpsU"
                     /locus_tag="K0W59_002727"
     CDS             17187..17402
                     /gene="rpsU"
                     /locus_tag="K0W59_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBI3023701.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17513..19258
                     /gene="dnaG"
                     /locus_tag="K0W59_002728"
     CDS             17513..19258
                     /gene="dnaG"
                     /locus_tag="K0W59_002728"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBI3023702.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVIFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTSGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19453..21294
                     /gene="rpoD"
                     /locus_tag="K0W59_002729"
     CDS             19453..21294
                     /gene="rpoD"
                     /locus_tag="K0W59_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBI3023703.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5157 a         5897 c         5483 g         4822 t
ORIGIN      
        1 actgtcgttc tcaaaatcgg tggagttgca tgacaaggtc atcgggcatt atctgaacat
       61 aaaacactat cagtaagttg gagtcattac cgaacaacaa gaagcgttta tgacgcttga
      121 gcaggagcag caggttaaaa cccgtactgc cgaacagaat gcacgtattg cggcttttga
      181 agctgaacgt cgtcgtgaag cagagcagac gcgaattctg gctgaacgac aaattcagga
      241 aacggagatc gagcgcgagc aggccgtccg ctcaagaaag gttgaggctg aacgtgaagt
      301 tcgtattaaa gagatcgaac agcagcaggt caccgaaatc gccaaccaga cgaaatcgat
      361 cgctattgcc gccaaatcgg aacaacagtc ccaggcagaa gcgcgtgcta accttgcact
      421 cgcagaagca gtaagtgcgc aacaaaacgt agaaaccact agccagaccg cagaagccga
      481 tcgtgctaaa caagttgccc taatcgctgc cgcgcaggat gcagaaacca aagcggttga
      541 actgaccgtg cgggcgaaag cagaaaaaga agccgcagag atgcaggcgg cggctatcgt
      601 tgagttagcc gaagctacac gtaaaaaggg tctggcggaa gcagaagcac aacgtgcgct
      661 gaacgatgct atcaacgtac tttctgatga acaaaccagc cttaaattca aactggcctt
      721 gttgcaggcg ctacctgcgg taatagaaaa atccgttgag ccgatgaaat ctattgacgg
      781 tatcaagatt attcaggtcg atggtctgaa tcgtggcagc gctgcgggtg atgcaaacac
      841 gggtaatgtg gggggcggaa acctagcgga acaagcatta tcagccgctc tctcttaccg
      901 cacacaggct ccgctgattg actccttgct caatgaaatt ggcgtttcag gcggttcact
      961 ggcgacattg acttcaccct taacctcaac aactcccgtc gccgaaaacg tagaataaat
     1021 tttcagctat gaacgtccct ggcacaaaaa gccggggatt ttttatatct gcgttccgct
     1081 aaaaactgca aatgatcagg tcgttgcaac gttttgcgtg accgctcggg gaaggcaaaa
     1141 ctgcctctgg gaaagcgttg cgcgaggtcc ggcgctcatc aacaatcggg gagtcagcaa
     1201 agggctgaaa cgggaaagcc cctcccgagg aaggggcctt acagaaggaa agggttatga
     1261 tgaagctcgt catcatactg attgtgttgt tactcgtaag tttcgcagct tattaacagc
     1321 caatcagagg ggggagaaat cctccctcac ccttgttcct ttactttagg ttgaaaaaaa
     1381 atcagcgtca agaaggtttc attaacacat atccctggcc caaaaagccg gggatttttt
     1441 atatctgcgt tccgctaaaa ggtgcaaatg ctcaggacgt tgcagcgttt tgcgtgaccg
     1501 ctcggggaag gcaaaactgc ttcagggaaa gcgttgcgcg gggtccggcg ctcatcaaca
     1561 atcggggagt cagcaagggg ctgaaacggg aaagcccctc ccgaggaagg ggccttatag
     1621 aaggaaaggg ttatgatgaa gttcgtcatc atactggttg tgttgttact gttaagtttc
     1681 ccgacttact aacaactgat cagagggggg agaaatcctc ccttaccctt gttcctttac
     1741 tttaggttga aaaaacaaca gcgtcaatag gcctgcctgc tacgaagcga gatctgtgaa
     1801 ccgctttccg gttagccttt tttatcctgt tggatcttct tgatgatgtt agtggtcgag
     1861 caaccgtctt cgaagttgag caccagcact tcgccgccgt tggcccagac ttctttactc
     1921 ccggcgatct cttctggttt atagtcgccg cccttcacca gtaaatctgg caggatcccg
     1981 gcgatcaagc gctgcggcgt gtcttcttca aacgacacca cccagtcgac cgcttccagt
     2041 gcgcccagca caatcatacg ctgttcgagc gggttaacgg ggcgggaatc ccctttcagc
     2101 cgtttggtgg aggcatcgct gttcacagcg acaatcaagc ggtcacccag tttgcgggca
     2161 tttgccagat agaagacgtg tccggcgtgc aggatgtcaa agacgccatt ggtcatcacc
     2221 actttttcac cgcgtttacg cgctgccgct acggccagct tcagttcctc ttcggtcatc
     2281 acgccaaagc cggtatctgc gcgcccacgc actgcatttt ccagctcgat cggcgaaacc
     2341 gtggaggttc ccagtttgcc gaccaccacg ccagccgccg cattggcaaa gaagcaggct
     2401 tcttccagcg aattacccgc tgccagcgtt gccgccagga cgccaatcac cgtgtcgccc
     2461 gcaccggtaa cgtcatacac ttcctgcgcc tgggttggca tatgcagcgg cgctttaccc
     2521 ggttgcagca gcgacatacc ctgttcggaa cgggtcacta acagagccga gagttcgtaa
     2581 tcggcaatca gtttcatgcc gcgctcaaca atctcttctt cggtcttaca tttaccgaca
     2641 acagcttcaa attccgagag attcggtgtc agcagcgtag cgccgcggta gcgctcaaaa
     2701 tcggtacctt ttggatcaat cagcaccgga acgcccgctt tacgcgccag ttggatcatc
     2761 tgctgtacgc ttgccagcgc acctttggcg tagtcagaaa gcaccagcgc gccaatcgaa
     2821 ctcagcgcct gattaatccg ctcgtgcagc ggctgcggat caacaccttc gaaaccttct
     2881 tcaaaatcca gacggatcag ctgttggttg cgggaaagta cccgtaattt ggtgatggtc
     2941 ggatgcgtcg gtacagaaac gaagtcgcat ttgacgttga cgtcggccag agatttactc
     3001 agcgcgcgcg ctgcatcgtc aatgcccgtc aacccgacca ggcgtgcatt agcaccgaga
     3061 gaagcgatat tcatcgccac gttagccgcg ccgcccggac gttcttcgat ggtattcact
     3121 ttaaccacgg gcaccggcgc ttccggcgag atacgactgg tggggccgta ccagtaacga
     3181 tccagcatca catcaccaac caccatcact cctgcacgtt caaactctgg cagcgttact
     3241 ttcattcctg tctcctgaga gattcaaaat ttgcgcgcga taataccata cttcattctt
     3301 ccaccagcca cttctgccag cttgcccgca ccagatcacg ctctgcggtg aagcaatcct
     3361 ccgacacatg gcccggcaat tcctgtaatg ccagatgatg aagttcatcg cgcagcgtcg
     3421 tgtaagcacg ggtcagcgcc atcgcttcct gctcttccat aatgtcgttt tgcgccagca
     3481 gctccagaat acgcacgttg tctgaccagc gcgttaactt cggtttttca tgagcgtagc
     3541 gcaacaccag atattgggta ataaattcga tatcggtaat tccgccttca tcggctttga
     3601 tatcaaagcg attacgatgt ttattgccga gatgagcgcg cattttctcg cgcatttccc
     3661 gcacttccgt ttgcagagtt ttaccttctc gcggcagtgt cataatctca cgacgcaccg
     3721 cgtcaaagtg cgcagtgagc tgcggatcgc cgtacactac acgcgcacgc accagcgcct
     3781 gatgttccca cgtccaggcc tcgtttttct gataatcggc aaatgctgct gcggatgtca
     3841 ccagcattcc cgccgccccg gacggacgca gtcgagcatc cacttcatac aaaatgccgg
     3901 aagaggtacg cgtactgaac agatgcataa tgcgttgcgc cagacgcaga taaaactgcc
     3961 gcccgtcgat ttcccgctcg ccgtcagtca tcgcatccat tgggcaatca tggaggaaga
     4021 taaggtcaag atcggaactg tagcctaact cccagccgcc cagcttgccg tagccgacca
     4081 ccgcaaaacc gcgcccttca cgttcgttga ggtgatttgg cttaccgtag cgggccacca
     4141 tttgacccca cgcctgctga acgacggcat ctatcatagc ttctgccagc caggttaagt
     4201 gatcgctcac tttcattacc ggtagcgtac cggcgatatc cgctgccgca atgcgtaaca
     4261 gctgcgcctg tttgaactga cgcagcgcct caagctgttg ttcttcgtca tcttccggca
     4321 cgcgcagcaa atactggcgc agttcatcac ggtaggcatc ggtcgccgtc ggctggtaaa
     4381 gggtatttgg atcgagcaat tcatccagca ataatggata acgcgctagc tggctggcaa
     4441 tcatcggcga tgcggcacac agagaaatca aatgtttaag cgccgcaggg aattcactga
     4501 gcaattctaa ataggttgtg cgggtaacaa tccccaccag caaggcggta atgcgcgaca
     4561 gcgtgacagc agcgtcttca cgcgcacaga catcacttag cagatgtggc atcagatggt
     4621 cgagcacctg acgtcctcgc ggcccgatag tacgcttatc cagctctttg cggaaatcag
     4681 caatcagcgt taggacctgt ttgcgatcat cctcgctaag atgcgccagc actggcgtag
     4741 tgtcatcttc ctgcaacgca tcctgccaca gctcacgcca ctgttccgac agcgactctt
     4801 cctgagtttc actttcgtcg tcgccaatca attcattaaa cacccggcgc acattggtca
     4861 tatgtgcggt cagcgccccg gtcagttgcg gccagtcagc aaagtccatc gcccacgcca
     4921 gccgcgcacg gttaagctca tcagaaggaa gcgtttgggt ttgttcgtcg ttaatgcttt
     4981 gcagcaggtt ttccagacgc cgcaggaaca gatatgccac tcgcaattgt tcagcatcgt
     5041 tttcagaaag cagatgcagc gcggcaatgg cgctgagcgt tggcagtaaa gagcgcgact
     5101 gcagcaacgg ttcgcgccca ccgcgaatga gctggaacac ctgaacgata aattcaattt
     5161 cgcgaatgcc gcctgcgccg agtttgatat tgtcggtcaa accacgtcga cgcacttcac
     5221 gggcaatcat ccctttcatg ttgcgcagcg actgaatcac gctgaaatcg atgtaacgac
     5281 ggaaaacaaa cgggcgcagc atcgcacgca actcgttagc atagacgcct tcgctatcgc
     5341 ccataatccg cgctttgacc atcgcgtaac gctcccagtc gcgtccctgc tcctggtaat
     5401 aatcctccaa cgcggcaaag ctcagcacca gcgggccact ttcgccaaac ggacgcagtc
     5461 gcatatccac gcgatagacg aagccatcct gcgttggttg atccagcact ttaatcagcc
     5521 gctgccccat gcgggtaaaa aactgcgcgt tatccagttc ccggcgtccg ccctgcgtac
     5581 aaccatgttc cggccaggca aaaatcagat cgatatcaga ggagaaattt agttccccgc
     5641 cgcccagctt acccatgcct aagatcagca gcggttgcgc ttcgccctgc gcattgcacg
     5701 gcgttcccca ctcgcggcag caggcgtcat acagccagtc acgcgccgca acaatcagcg
     5761 tctccgccag ataactgagc tgctgcaata tgctctcttc agtaaccagt gccagcgttt
     5821 gcgcccatgc gatgcgcacc ataatgcgcc gccggaatag ccgcagctcg cgcattaacc
     5881 cggcttcgtc gctcacatta ctgagcgcct cctgcaacca cacgctgtaa tgttgccatt
     5941 cgtcagcctg tggcggttgg ctttccagtt ccgtcagcca ctctggatgc gcaatcacgc
     6001 tgtcctgcac aaaatcactg aaagtaagta ctgacttcgc ctgtgcacta agtgattcct
     6061 cggctaaaag ctctggcagc cgctcaacaa cggtctgcca gtactgctgt aacggtgaag
     6121 agagcggctt cataagcgat tttatccttg cctgattaac gttttccgct gtgcaaccag
     6181 aacggttcct gattgtttgc ctcattacgg aaatgttcaa tttcaatgcg ttgcccggtc
     6241 gcaatagcgt gacgcagccc ctgccagttc tccagccagg cttgcgcgac gacaggatca
     6301 tagtaacccg ccagcaacag tattgagtca atatcacgca tcaggcgtgg caactggtca
     6361 cggtagcgat cgcctaacgg ctggcagaaa acgcttttca gttcagcggc atggcgggaa
     6421 agatggatat cggcaaagcg tttgaaggag tcgctaattt tgccctgcgc tttggcatct
     6481 aaaaacggct gccatgcttt gcttaccagc cattcggtca acgccagctt cgccattgcc
     6541 gtttcggtag agtagaccgc cgtcacggca gaaaccgcag aagcgatggt cgcttcgcat
     6601 tgaatcagca aatcacgtaa gtgagtgctc gctttacgcg gcacaatgcc accgaacagc
     6661 atcagtgtat gacggactag gctaatggct gccagcacct gctctttcgc cgcatcgttg
     6721 ccgcgtaccc acagttcttc atgatacagc cattgcgcta acaccagctc gagcgccgct
     6781 tccagcccct gttccacatc ggcttttgcc gcaacatgca aaatggtggt cggtttgatt
     6841 tcacgcgccg gattgccctg cgccagatga tagccacgcg ccgctttgct caggctgccc
     6901 tggcgtaatc cggtttgcga taccagttgg ttcgccagtt tcagcaccgc gcgcgtgtcg
     6961 ccgctaagca gctccagttc cagctcgcag ataggctcag caaattcacc cgctttcact
     7021 tccccctggt cgagagcgat ttcaattcgg ctgccatcga tctctactag ccatttttcg
     7081 cgataaaaat cggtgctaaa cagcggctgc acgcgggaag cgagatcggc aggcaattcg
     7141 ccgttcggcc agatttccgt cggtaactgc gccaggtcga gcgtcggttc gctcaacgcc
     7201 acgttatatt ccgggcgctg gtgtaagccg cctgtcactc ttcctgcaac tttcatggtc
     7261 atctcatagc gaccgttttc gccacgaata cgtaagccca tatcgtgcct acgcagccag
     7321 ttatccggcg tttcgtagta aatattcagc aactgcacgg ggtcatggtg ctcgccgccc
     7381 agcgtattga gatggtcacg caacgcctca acggcactgt gattaacaat aaactttaat
     7441 tcgatttcct gagccatggc ctttacttat cggttatgtc acataagtga cgatgaacgg
     7501 cgaacttaat gcgatctttt ttgtcagtag atagtatttt gcgccaaatt gccatgcaac
     7561 gggcaatttg acgggcgtaa aagtttgaag cagtggcaag acctgacaca gaggattccg
     7621 attgatgtcg aatgctttgc gtagcgacac tgatgccact actatcgttc cactttccat
     7681 gacaataacg acagcctgat gccaaaatta cgcctgatcg gattaacttt actcgcactt
     7741 agcgcgactg ccgtctcaca cgctgaagaa acgcgctatg tttctgacga actgaatacc
     7801 tgggtccgta gcggtccggg agatcattat cgcctcgtgg gcacggttaa cgccggcgag
     7861 gaagtgacct tattacaaac tgacgccaac accaattatg cccaggtgaa agacagctct
     7921 ggccgtaccg cctggatccc gttgaaacaa cttagcactg agccaagcct gcgctcccgt
     7981 gtgccagatc tggaaaatca ggtcaaaacc ctgaccgata aactcaccaa tatcgataac
     8041 acctggaatc agcgcaccgc agaaatgcag caaaaagtgg cgcagagcga cagcgtgatc
     8101 aacgggttaa aagaagaaaa tcagaaactg aaaaacgagc tgattgtcgc gcagaaaaag
     8161 gtcgatgccg ccagcgtaca gctggatgac aaacagcgca ccatcatcat gcaatggttt
     8221 atgtatggcg gtggcgtgct ggggcttggc ttgctgctcg gtctggtact gccgcacctg
     8281 atcccaaacc gcaaacgcaa agatcgctgg atgaactaaa tcgccttctt cgttgcactg
     8341 tcttattatt aggaattgtg tatttgacga agggatgatg gcgtgaagat ttatctggtc
     8401 ggtggtgctg ttcgggatgc attgttaggg ctatcggtca aagacagaga ttgggtggtg
     8461 gtcggcagta cgccacagga gatgctcgac gcgggctacc agcaggtagg ccgcgatttt
     8521 cctgtgtttc tgcatccgca aacgcatgaa gagtatgcgc tggcacgtac cgaacggaaa
     8581 tccggttccg gttacaccgg ttttacttgc tatgccgcac cggatgtcac gctggaagat
     8641 gatcttaagc gtcgcgatct gaccattaat gcgctggccc aggacgataa cggcgagatt
     8701 atcgacccgt acaacggtct gggcgatctg caaaatcgtc tgttgcgcca tgtttccccc
     8761 gcttttggcg aagatccgtt acgcgtactg cgcgtggcgc gttttgctgc gcgttatgcc
     8821 cacctcggtt ttcgtattgc cgatgaaact ctgacgctga tgcgcgagat gacccatgcg
     8881 ggtgaactgg aacacctgac gcctgaacgg gtatggaaag agacggaaag cgccctcacc
     8941 acccgcaatc cccaggtgtt cttccaggta ctgcgcgatt gcggcgcact tcgcgtttta
     9001 ttcccggaaa ttgacgcact gtttggcgtt ccggcacctg ccaagtggca tccggaaatc
     9061 gacacgggta ttcatacctt aatgacgctc tcaatggcgg cgatgctgag tccgcaggtt
     9121 gatgtccgtt tcgcaacttt atgccacgat ctcggtaaag gactgacgcc gccagaactt
     9181 tggccgcgtc atcatgggca tggcccggcg ggtgttaagt tagtggaaca actatgccag
     9241 cgtctgcgcg tgccaaatga aattcgcgat ttagccagac tggtggctga gtttcacgat
     9301 ctcatccaca ccttcccaat gctgaaccca aaaaccatcg tcaaattatt tgattccatc
     9361 gatgcctggc gtaaaccgca gcgtgtcgag caactggcgc tgaccagcga ggctgacgtg
     9421 cgcggcagaa ccggttttga atcagcggac tacccgcaag gccgttggtt gcgcgaaggc
     9481 tgggaagtgg cgcagtcagt gccgacaaaa gcggtcgttg aagcgggatt taaaggcgtg
     9541 gagattcgcg aggagctgac ccgacggcgg attgcggcgg tagccagctg gaaggaacaa
     9601 cgttgcccaa agcctgactg agtataaaac taaacgttag cacgaaccgt cccctcgccc
     9661 cttcggggag agggttaggg tgaggggaac agaccagcac cggtgcgaac aatcaccccc
     9721 accccggccc tctccctaca gagcgaggga gaaaacccgg cctgctcccg aaccttttct
     9781 caatcaaaag aacacgacat acaccgcagc cgccacaata aagcgataaa tggcgaacgg
     9841 gataaacgaa atgcgcttaa tcaattgcag gaaggtttta atcgctatca gcgccaccac
     9901 aaaagcggtg ataaacccaa cggcaaacat cgggatatcg ccggttgtca ggaagcccca
     9961 gcttttgtag agatcgagcg ccgttgcgcc catcatcatc ggtaccgcca gcaggaacga
    10021 gaactcggaa gcagcgtaac ggctcacgcc catcagcatc ccacctgaaa tggtcgcccc
    10081 ggaacgggag aaacccggcc acagcgccag acactggaaa cagccaatca taaacgcctg
    10141 acgataggtc atatcatcaa gacccggcgc acgcggctct ttcggcttca ggcattcggc
    10201 ggcaatcagc aacaaaccgc caacgaccag cgcatacatc acatttatcg ggttaaacaa
    10261 tgacttaatt gtgtcgtgga ataatagccc cagtaccacc gccggaatca tccccagcaa
    10321 aatgtggatc agcgttaaac gacctttgct ttcaccttcg tgctgcaacg ggcggccaaa
    10381 gtggatgcca atcaggccaa acagacgccg ccagaacatc actactaccg ccagaattga
    10441 tcctaactgg atcacaactt caaaggtttt cgccgtgtcg ccctcaaatc ccaacaagtg
    10501 accgacaata atcatatggc ccgtgctgga taccggcaga aattctgtca atccttcgac
    10561 cacacccaat attgccgcta tcagcagcga gtgcatatcg ctcatcaata aacccctaaa
    10621 ttattaaaat gtaccgcttg tccgaactac tgcagtatga ccaggttata accgtttggt
    10681 ttaacagctg taaaattaat tattttcttt cagattattg ccacgctcaa tgattacgcc
    10741 aacattcgcc gcccgcgcca ctgcgcctgg cttgctgagt ttgatacgca cccacggcga
    10801 gttgaagcgt gctaacagca gctccgccac ctcttcagcc acgcgttcca ccagcgcaaa
    10861 acgcgccccc tcgacgtggc tgaccaccgt ttctgcaatg tcagcgtaac tgaggcaatc
    10921 cgccacatca tcacttttcg ccgctttacg gttatcccat gccatttcga tatcgaacac
    10981 taacttctgt tcgatggtct gttcccagtc gtaaacacca atagtggtga ttaccgaaag
    11041 ttgctctata aatacaatat ccatcacgtc ctgcctgctt tttggctaac cggataccac
    11101 ttccggcgaa atgtgcgtat tatccacaga ttcatcgttg aacacgaatt ttcaaaacgg
    11161 aacagcttat gagtgcaatc gcgcctggaa tgatcctcat cgcgtacctc tgcggctcca
    11221 tttccagtgc cattctggtt tgccgcttgt gtgggctgcc cgatccgcga accagcggct
    11281 ccggcaatcc aggcgcaacc aatgtgttac gtatcggtgg caagggagca gccgtagcag
    11341 tactgatttt cgacgttctg aaaggaatgt tgcctgtctg gggcgcgtat gaattaggtg
    11401 tcagcccctt ctggctaggc ttaattgcca tcgccgcctg tcttggacac atctggcccg
    11461 ttttcttcgg ttttaaagga ggaaaaggcg ttgctaccgc ttttggagca atcgcaccaa
    11521 ttggctggga tctcaccgga gtaatggcgg gaacctggtt actgaccgtg ctattgagcg
    11581 gatactcgtc gctgggagcg attgtcagtg cactgattgc tccgttttat gtctggtggt
    11641 ttaagccaca attcaccttc ccggtttcga tgctctcttg cctgatcctg ctgcgtcatc
    11701 atgacaacat ccagcgtctg tggcgtcgtc aggagacaaa aatctggacg aaattcaaaa
    11761 gaaagcgcga aaaggatccc gagtgatttc tggtggatct acatgacctg atagccttca
    11821 tcgggcttgc ccagccgttg ctggcaccat gccgccagaa attccacgca gacacgaatt
    11881 tcatgctgcg atagagcggc tcccggtaaa cagcccagat attggcgctt tgtgcatact
    11941 ccggcaatac ttgcaccagt ttgccactct ccagaaacgg caacacatcc cactcggaac
    12001 gcagcataat ccctttgccc tccagcgccc attgcaacac tatctcgccg ctattggagg
    12061 aaagatgccc gctaaccttc accgattttt tctcctgacc gttccccaac tcccatattc
    12121 cgtgggtcat atcgcgctct ttggtcacca gacagtcatg acgacttaat tcctgtaatg
    12181 attgtggctg cggatatttt tgcagatact caggtgctgc gcataatatt cttttatttt
    12241 tcgttaacag atgcgcaata taataatcag gaatttcgtc attaatacga atatccagat
    12301 caatattatc ctgcactaaa tcaatttgcc gatcgaacaa ttcaaaatgc acctgtaact
    12361 caggataatt gcgcataagt tcggtaatgg ctggcgcaat atggctgcgc ccaaaaccaa
    12421 aactgcaacc aatacgaatc atcccctccg ggcgcgtttt gatttgcgtg acgtcatcga
    12481 ccagtcgctg atattgggtg aggatttcca gcgcatgttc gtaacaacgt tggccacttt
    12541 cagtcagcgc cacgccgcga gcggagcggt tcagcagcgt tgttgccagc gtattttcaa
    12601 ggatttggat ccgcttggtg acaaatgcag gcgtttgccc aagcgtcgcc gctgcagcac
    12661 taaaactccc gctgtggacg atttctacca gtacctgcaa gtctttggct aaaggccagc
    12721 tattcagcat cgttaattat ccgcagttgt gataagcgca gtgtatttcg caaaacattg
    12781 ccattccctg ctctggcgca atattcattc ttattttcgc gttgattcac gaactgttaa
    12841 ttacatattc acggtggcaa aaaatataaa accacatttt tagtggtagt ttgtggcggt
    12901 gaatttttcc agacaaatac aaaaactgga gttgccatga tgagcgaaag taataagcaa
    12961 caggcagtga ataagttgac agagattgtc gctaacttta ccgccatgat ttctacccga
    13021 atgcctgatg acgtggtgga taaactaaaa cagctaaagg atgccgaaac gtcgtcgatg
    13081 gggaaaatta tctaccatac gatgttcgac aacatgcaaa aagcgattga cctgaatcgt
    13141 cctgcctgtc aggacaccgg ggagattatg ttcttcgtta aagtcggttc ccgcttccca
    13201 ctgcttggcg agctgcaaag catactcaaa caagccgtgg aagaggcaac cgtcaaagcg
    13261 ccactacgtc acaatgcggt agaaattttt gacgaagtaa acaccggcaa aaataccggt
    13321 agcggcgtac cgtgggtcac ctgggacatc atccccgaca atgacgatgc ggaaatcgaa
    13381 gtttacatgg caggcggcgg ctgcacgcta cctggccgct cgaaagtgtt aatgccgtca
    13441 gaaggctacg aaggcgtggt gaaattcgtc ttcgaaaata tctccaccct cgccgtaaac
    13501 gcctgtccac cggtactggt gggcgtgggc atcgccacct cggtggaaac cgccgccgta
    13561 ctctcgcgta aagccattct gcgcccgatt ggcagccgcc atcccaatcc aaaagcggca
    13621 gaactggagc tacgcctgga agaaggactc aaccgtctgg ggattggtcc acaagggctg
    13681 accggcaaca gttcagtgat gggcgcatcg tcgcggcacg ctgctggttc atgccgatct
    13741 cacctttgaa aatctgtctc acacccggag cgcgttatga aaaagatcct gacaacctcg
    13801 atcaaagctg aagatctgca agatattcgc gtcggcgatg tgatctacct gaccggtacg
    13861 ctggtgacct gccgcgacgt ttgtcaccgc cgtttgatcg aactgaaacg tccgatccct
    13921 tacgatctca acggcaaagc gattttccac gctggcccca tcgtgcgcaa aaacggcgac
    13981 aaatgggaga tggtctccgt cggcccgaca accagtatgc gtatggaaag ttttgaacgt
    14041 gaatttattg agcagaccgg cgtaaaactg gtggttggca aaggtggtat ggggccgctg
    14101 accgaagaag gctgccagaa attcaaggcg ttacatgtga ttttcccggc aggctgcgcg
    14161 gtgctggcgg caacccaggt agaagagatt gaagaagtgc actggacaga actcgggatg
    14221 ccggagtcac tgtgggtctg ccgggtcaaa gagttcggcc cgctgattgt ctctattgat
    14281 acccacggca acaacctgat agccgaaaac aaaaagctgt tcgccgaacg ccgcgatccc
    14341 atcgtggaag agatctgcga gcacgtccat tacatcaaat aaccctcccg gagaggctca
    14401 cccctctcct ttttcgcagg cataacacga tgaaaccttc cactgaatgg tggcgatatc
    14461 ttgcaccgct ggcggtcatc gccattattg ctctacttcc ggttcccgca gggctggaga
    14521 accatacctg gctctacttt gccgttttta ctggcgtgat cgttggactg atcctcgaac
    14581 ccgtgccggg tgccgtggtg gcgatggtgg gtatctccat catcgccata ctctctccct
    14641 ggctgctgtt cagccccgaa cagctcgccc agccaggctt taagttcacc gccaaatccc
    14701 tctcctgggc agtttctggt ttttctaatt cggttatctg gctcattttc gccgccttta
    14761 tgtttggcac aggctatgaa aaaaccgggc ttggacgccg cattgcgctg attctggtga
    14821 aaaagatggg gcatcgcacg ctgtttctcg gctatgcggt gatgttctcc gagctgatcc
    14881 tagcacctgt aacaccgtcc aactcggcgc gtggtgcggg tattatctat cccatcatcc
    14941 gtaacctgcc accgctctat caatcacaac caaacgacag cagttcgcgc agcattggct
    15001 cgtacatcat gtggatgggg attgttgccg actgcgtgac cagcgccatt ttcctgacgg
    15061 cgatggcacc taacttgctg ttaattggac tgatgaaaag cgcatctcac gccacgctga
    15121 gttggggcga ctggttcctc gggatgttgc cgctcagcat tttactgctt ctgctggttc
    15181 cctggctggc ttacgtgcta tacccgccgg tactgaagtc tggtgatcag gtgccgcgct
    15241 gggcagagac ggaactgcag gcaatgggcc cgctctgttc gcgtgaaaaa cggatgctgg
    15301 ggctgatggt aggcgcgctg gtgctgtgga ttttcggcgg tgattatatc gatgccgcga
    15361 tggtcggtta cagcgtggtg gcgctgatgc tgcttctgcg cattatcagt tgggacgaca
    15421 ttgtcagtaa taaagcggca tggaacgttt tcttctggct ggcctcgctt atcaccctcg
    15481 cgaccggact caacaatacc ggttttatta gctggtttgg caaactgtta gcaggcagct
    15541 taagtggtta tccgccgacg atggtgatgg tgacgttgat tgtggtgttt tatctactgc
    15601 gctacttttt cgccagcgcc acggcgtata cctccgccct cgcaccgatg atgatcgccg
    15661 ccgcgctggc gatgccggaa atcccgctgc cggtgttctg cctgatggtt ggcgcggcaa
    15721 ttggtctggg gagcattctt acaccatacg ccaccggacc cagtccgatt tactacggta
    15781 gtggttatct gccaacggcg gattactggc gactgggggc gatttttggg ctgatattcc
    15841 tcgtgttgct ggtgattacc ggcttactgt ggatgcccgt ggtgttgctt taagtgagga
    15901 cggtgccgga aattgctctc cggcacccaa tagttttacg cagccggtaa ctccgccagc
    15961 ggccagcgcg gacgcacgct aacgccgaga tccgccgtcg cgcctgcttt aaaccgcacc
    16021 attccggcat aggcgatcat cgcgccgtta tcagtacaaa attccggacg cgcgtagaac
    16081 acttcgccgc ggcgtttttt catcatttca gccagcttcg cccgtaacgt gcggttagca
    16141 ctcacgccgc ccgccatgac cagtcgctta aagcccgtct gatccaacgc tcgcttacac
    16201 ttaatcatca acgtatcgac caccgcatct tcaaaggcgc gggcgatatc agcacgcgtc
    16261 tggtcgtcgg tgctgttgtc acgaatcgtg ttcgccgcaa aggttttcag accagaaaag
    16321 ctgaaatcca gccccggacg gtcggtcatc ggacgcggga aaacaaagcg cccggcagta
    16381 ccctgagccg ccattttcga cagtaacggt ccgccaggat aatccagccc cagcagcttc
    16441 gcggttttat caaacgcttc accggcggca tcatcgatag actcgccgag cagctcgtac
    16501 tgaccaatgc cagtcacgct gattaactgc gtatgaccgc cggacaccag cagcgcgaca
    16561 aacggaaatt ccggcgggtt atcttccagc atcggcgcta acagatgccc ttccatatgg
    16621 tgtaccggga ttgccggaac gttccaggca aacgccagag agcgccccac ggttgcgcca
    16681 accagcagtg cgccgactaa tccagggcct gcggtatagg ccacagcatc aatgtctttt
    16741 gccgttaaac cagactcctt tagcgccgcc tggatcaacg gtacggtttt acgcacatga
    16801 tcgcgggagg ccagttcagg cacgacgccg ccgtagtcag cgtgcaattt cacctgacta
    16861 tacaattggt tggctaacaa acctttttca tcgtcgtaaa tggcgatgcc ggtttcatcg
    16921 caggaagttt caatacccag tacacgcatg acttgtttta cctcgcttta ttaccgcgca
    16981 gtgtaggacc aatgcgggtt gatgtaaaac tttgttcgcc cctggagaaa gcctcgtgta
    17041 tactcctcac ccttataaaa gtccctttca aaaaaggccg cggtgcttta caaagcagca
    17101 gcaattgcag taaaattccg caccattttg aaataagctg gcgttgatgc cagcggcaaa
    17161 ccgaattaat caaaggtgag aggcacatgc cggtaattaa agtacgtgaa aacgagccgt
    17221 tcgacgtagc tctgcgtcgc ttcaagcgtt cctgcgaaaa agcaggtgtt ctggcggaag
    17281 ttcgtcgtcg tgagttctat gaaaaaccga ctaccgaacg taagcgcgct aaagcttctg
    17341 cagtgaaacg tcacgcgaag aaactggctc gcgaaaacgc acgccgcact cgtctgtact
    17401 aattccccga gagcgttgct ctccgatcag accgagttgt agttgtaagg ccgtgcttcc
    17461 gaaaggaatg cgcggcttat tttcgtttat gaattgctaa aaatcggggc ctatggctgg
    17521 acgaatccca cgcgtattca ttaatgatct gctggcacgc actgacatcg tcgatctgat
    17581 cgatgcccgt gtgaagctga aaaagcaggg caagaatttc cacgcgtgtt gtccattcca
    17641 caacgagaaa accccgtcat tcaccgttaa cggtgagaaa cagttttacc actgctttgg
    17701 atgtggcgcg cacggcaacg cgatcgactt cctgatgaac tacgacaagc tcgagttcgt
    17761 cgaaacggtc gaagagctgg cagcaatgca caatcttgaa gtgccatttg aagcaggcag
    17821 cggccccagc cagatcgagc gccatcagag gcaaacgctt tatcagttga tggacggtct
    17881 gaatacgttt taccaacaat ctttacaaca acctgttgcc acgtctgcgc gccagtatct
    17941 ggaaaaacgc ggattaagcc acgaggttat cgctcgcttt gcgattggtt ttgcgccccc
    18001 cggctgggac aacgtcctga agcggtttgg cggcaatcca gaaaatcgcc agtcattgat
    18061 tgatgcgggg atgttggtca ctaacgatca gggacgcagt tacgatcgtt tccgcgaacg
    18121 ggtgatattc cccattcgcg ataaacgcgg tcgggtgatt ggttttggcg ggcgcgtgct
    18181 gggcaacgat acccccaaat acctgaactc gccggaaaca gacattttcc ataaaggccg
    18241 ccagctttac ggtctttatg aagcgcagca ggataacgct gaacccaatc gtctgcttgt
    18301 ggtcgaaggc tatatggacg tggtggcgct ggcgcaatac ggcattaatt acgccgttgc
    18361 gtcgttaggt acgtcaacca ccgccgatca catacaactg ttgttccgcg cgaccaacaa
    18421 tgtcatttgc tgttatgacg gcgaccgtgc aggccgcgat gccgcatggc gagcgctgga
    18481 aacggcgctg ccttacatga cagacggccg tcagctacgc tttatgtttt tgccggatgg
    18541 cgaagaccct gacacgctgg tacgaaaaga aggtaaagaa gcgtttgaag cgcggatgga
    18601 gcaggcgatg ccactctccg catttctgtt taacagtctg atgccgcaag ttgatctgag
    18661 tacccctgac gggcgcgcac gtttgagtac gctggcactg ccattgatat cgcaagtgcc
    18721 gggcgaaacg ctgcgaatat atcttcgtca ggaattaggc aacaaattag gcatacttga
    18781 tgacagccag cttgaacgat taatgccaaa agcggcagag agcggcgttt ctcgccctgt
    18841 tccgcagcta aaacgcacga ccatgcgtat acttataggg ttgctggtgc aaaatccaga
    18901 attagcgacg ttggtcccgc cgcttgagaa tctggatgaa aataagctcc ctggacttgg
    18961 cttattcaga gaactggtca acacttgtct ctcccagcca ggtctgacct ccgggcaact
    19021 tttagagcac tatcgtggta caaataatgc tgccaccctt gaaaaactgt cgatgtggga
    19081 cgatatagca gataagaata ttgctgagca aaccttcacc gactcactca accatatgtt
    19141 tgattcgctg cttgaactgc gccaggaaga gttaatcgct cgtgagcgca cgcatggttt
    19201 aagcaacgaa gaacgcctgg agctctggac attaaaccag gagctggcga aaaagtgatt
    19261 taacggctta agtgccgaag agcgatcggg aagcccccga cagccgcact gagaggcagc
    19321 ggcaaatata taagtacgcc ctcgtaatta tcgttggcgg taaacaaccg ttggatttca
    19381 gcgttaacgg ctgaaggaca tcgggtcaat cgcccaacac caacctcatg aaataagtgt
    19441 ggataccgtc ttatggagca aaacccgcag tcacagctga aacttcttgt cacccgtggt
    19501 aaggagcaag gctatctgac ctatgccgag gtcaatgacc atctgccgga agatatcgtc
    19561 gattcagatc agatcgaaga catcatccaa atgatcaacg acatgggcat tcaggtgatg
    19621 gaagaagcac cggatgccga tgatctgatg ctggctgaaa acaccgcgga cgaagatgct
    19681 gccgaagccg ccgcgcaggt gctttccagc gtggaatctg aaatcgggcg cacgactgac
    19741 ccggtacgca tgtacatgcg tgaaatgggc accgttgaac tgttgacccg cgaaggcgaa
    19801 attgacatcg ctaagcgtat tgaagacggg atcaaccaag ttcaatgctc cgttgctgaa
    19861 tatccggaag cgatcaccta tctgctggaa cagtacgatc gtgttgaagc agaagaagcg
    19921 cgtctgtccg atctgatcac cggctttgtt gacccgaacg cagaagaaga tctggcacct
    19981 accgccactc acgtcggttc tgagctttcc caggaagatc tggacgatga cgaagatgaa
    20041 gacgaagaag atggcgatga cgacagcgcc gatgatgaca acagcatcga cccggaactg
    20101 gctcgcgaaa aatttgcgga actacgcgct cagtacgttg taacgcgtga caccatcaaa
    20161 gcgaaaggtc gcagtcacgc tgccgctcag gaagagatcc tgaaactgtc tgaagtattc
    20221 aaacagttcc gcctggtgcc gaagcagttt gactacctgg tcaacagcat gcgcgtcatg
    20281 atggaccgcg ttcgtacgca agaacgtctg atcatgaagc tctgcgttga gcagtgcaaa
    20341 atgccgaaga aaaacttcat taccctgttt accggcaacg aaaccagcga tacctggttc
    20401 aacgcggcaa ttgcgatgaa caagccgtgg tcggaaaaac tgcacgatgt ctctgaagaa
    20461 gtgcatcgcg ccctgcaaaa actgcagcag attgaagaag aaaccggcct gaccatcgag
    20521 caggttaaag atatcaaccg tcgtatgtcc atcggtgaag cgaaagcccg ccgtgcgaag
    20581 aaagagatgg ttgaagcgaa cttacgtctg gttatttcta tcgccaagaa atacaccaac
    20641 cgtggcttgc agttccttga cctgattcag gaaggtaaca tcggcctgat gaaagcggtt
    20701 gataaattcg aataccgccg tggttacaag ttctccacct acgcaacctg gtggatccgt
    20761 caggcgatca cccgttctat cgcggatcag gcgcgcacca tccgtattcc ggtgcatatg
    20821 attgagacta tcaacaaact caaccgtatt tctcgccaga tgctgcaaga gatgggccgt
    20881 gaaccgacgc cggaagaact ggctgaacgt atgctgatgc cggaagacaa gatccgcaaa
    20941 gtgctgaaga tcgccaaaga gccaatctcc atggaaacgc cgatcggtga tgatgaagat
    21001 tcgcatctgg gggatttcat cgaggatacc accctcgagc tgccgctgga ttctgcgacc
    21061 accgaaagcc tgcgtgcggc aacgcacgac gtgctggctg gcctgaccgc gcgtgaagca
    21121 aaagttctgc gtatgcgttt cggtatcgat atgaacaccg accacacgct ggaagaagtg
    21181 ggtaaacagt tcgacgttac ccgcgaacgt atccgtcaga tcgaagcgaa ggcgctgcgc
    21241 aaactgcgtc acccgagccg ttctgaagtg ttgcgtagct tcctggacga ttaatcggta
    21301 ggccggatca ggcgttacgc cgcacccggc actagtctct ctgcacaaac gccaccttt
//
LOCUS       DAETPZ010000064        21254 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.064, whole genome shotgun sequence.
ACCESSION   DAETPZ010000064 DAETPZ010000000
VERSION     DAETPZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.064"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(32..1012)
                     /locus_tag="K0W59_002730"
     CDS             complement(32..1012)
                     /locus_tag="K0W59_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBI3023704.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSALDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1020..1730)
                     /locus_tag="K0W59_002731"
     CDS             complement(1020..1730)
                     /locus_tag="K0W59_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HBI3023705.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(1947..3770)
                     /gene="typA"
                     /locus_tag="K0W59_002732"
     CDS             complement(1947..3770)
                     /gene="typA"
                     /locus_tag="K0W59_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HBI3023706.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4143..5552
                     /gene="glnA"
                     /locus_tag="K0W59_002733"
     CDS             4143..5552
                     /gene="glnA"
                     /locus_tag="K0W59_002733"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HBI3023707.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5725..6774
                     /gene="glnL"
                     /locus_tag="K0W59_002734"
     CDS             5725..6774
                     /gene="glnL"
                     /locus_tag="K0W59_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HBI3023708.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6786..8195
                     /gene="glnG"
                     /locus_tag="K0W59_002735"
     CDS             6786..8195
                     /gene="glnG"
                     /locus_tag="K0W59_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HBI3023709.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8307..8417
                     /locus_tag="K0W59_002736"
     CDS             8307..8417
                     /locus_tag="K0W59_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HBI3023710.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8646..10019)
                     /gene="hemN"
                     /locus_tag="K0W59_002737"
     CDS             complement(8646..10019)
                     /gene="hemN"
                     /locus_tag="K0W59_002737"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBI3023711.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10208..10717)
                     /gene="yihI"
                     /locus_tag="K0W59_002738"
     CDS             complement(10208..10717)
                     /gene="yihI"
                     /locus_tag="K0W59_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HBI3023712.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11299..11931
                     /locus_tag="K0W59_002739"
     CDS             11299..11931
                     /locus_tag="K0W59_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBI3023713.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12313..15099)
                     /gene="polA"
                     /locus_tag="K0W59_002740"
     CDS             complement(12313..15099)
                     /gene="polA"
                     /locus_tag="K0W59_002740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBI3023714.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15463..16395
                     /locus_tag="K0W59_002741"
     CDS             15463..16395
                     /locus_tag="K0W59_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBI3023715.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLISSQRQNK"
     gene            complement(16436..17866)
                     /locus_tag="K0W59_002742"
     CDS             complement(16436..17866)
                     /locus_tag="K0W59_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBI3023716.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRIQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNVVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(18021..18647)
                     /gene="dsbA"
                     /locus_tag="K0W59_002743"
     CDS             complement(18021..18647)
                     /gene="dsbA"
                     /locus_tag="K0W59_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HBI3023717.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(18664..19650)
                     /gene="srkA"
                     /locus_tag="K0W59_002744"
     CDS             complement(18664..19650)
                     /gene="srkA"
                     /locus_tag="K0W59_002744"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HBI3023718.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQESPLQLTPMY"
     gene            complement(19727..19996)
                     /locus_tag="K0W59_002745"
     CDS             complement(19727..19996)
                     /locus_tag="K0W59_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HBI3023719.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20066..20650
                     /gene="mobA"
                     /locus_tag="K0W59_002746"
     CDS             20066..20650
                     /gene="mobA"
                     /locus_tag="K0W59_002746"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HBI3023720.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20632..21159
                     /gene="mobB"
                     /locus_tag="K0W59_002747"
     CDS             20632..21159
                     /gene="mobB"
                     /locus_tag="K0W59_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBI3023721.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDAAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5181 a         5351 c         5122 g         5600 t
ORIGIN      
        1 gttttattaa ctgcgtatga gttaaaactt attagtggtt cagaagatgg tttgcctctt
       61 gtttgatttg ttgtagtacg ttacgcacat gatttatctg attaattgca tcttcttttt
      121 ctttacgtaa ccgcgcatcc agtaaatgat tttctggtag gggtgtttca ctcatctggc
      181 gataagggat ccacggatta tcatcttcgt cttcaaagcg gaacgccggc gcatagtaat
      241 caacctcttc ttccaggcca tatgccgtca cctgcggctc atcatcacca aggcagataa
      301 ggtgcgttaa taacgctttg aatggcatat caccctgacc tgaaatacag gctttatgac
      361 ccaggccacc ctgttcttta acgatcaagg catctttaat atggacctgg gtaatatgcg
      421 gtgccatcgt ttttaaggcg tcgatgggat gctcatttgc attgatcatg ttagcaaaat
      481 caaacagtaa ggaaagagac tccatctcac tttctttgac cagcgacacc agctcatgac
      541 tttttaaatc ttcatgctgc tcaatcgtaa aagtcaggcc gctgtcctga tacgtttccc
      601 gtacataggc aatgtcgtta gcgataatcg ataatacgtc gcgcaaatta ccttcataac
      661 gtggataaaa acgtacggac gttgccccag ttttcaacgc gatggcgacg gcttcgtcaa
      721 tagatgcctt atctaaagcg ctggtttcaa tatgaatatc aaggttcaga cggcgggctt
      781 tatccccaaa ggcagagagt tctttatcgt ccatattacc aagagaaaaa cgctcgccat
      841 ccagaacatg gattttgacg ccacgtagat tattttcact tgcgatatca agtaaatcag
      901 caggcaacac tctttccagc cgcatattta aatgaaaggc ataagcatgt aaataaagag
      961 gaagagtgtc gacacgttgt agaatctttc ttgcgttatt tattgtcacc ataaaacacc
     1021 tacagattaa tgatgaggtc tacggcctca aatcgccaat attcaatatc tatctggaca
     1081 atattgcctt gctggtcggc gcgatgcttt tcaattaaga tcgctggcat acccgcagaa
     1141 ccgccaagat acttgctgat atcgcccggc attctgacgg gtttgaaaat caattttttt
     1201 actaccgtct ctttttggta gcacttttcc cagactgcag aaaatgagtg gttttcaagt
     1261 tgttcaataa aacccggagc aacttcagga ttaatatatg tttcgtgata gaaaacttta
     1321 tgtccttcca gcgcccccca gccggtgatg cgatagagtt cagttgatgg cgtcactgca
     1381 atcagtggcg cgagcgtctc gggaatatcg ctggtacggt ttttctcggt aaacccccaa
     1441 gaaggctctc gcccttgctc aattgcggcc cgctgaaagc tcgccgacag ctccggactg
     1501 taattaaatc gcggctgggt cacgaaccac cccttacgat ccttacggaa gattttggat
     1561 tctgcctcga ggttcaacaa cgcctggcgc agcgtcatac gtttaatgcc cagcagttct
     1621 cccagctctc tttctgaagg gagttttcca cccggcgtgg tgatgccatc tttcaaccac
     1681 cgatccagtt tctcttttgc attttctacg gtggattgat tctccgccat tgtttaatcc
     1741 ttttggttta aaccagatga agcatcttac gcttacgcca aaaaagaaag caattaacgg
     1801 tgtatgactt tgcagataat tgtgacaaag ctctctattg acgtaatcac agacttataa
     1861 gggagatact cgcattggtg tgcgctcacg acaggtaaaa aaaaacctgc catcgctggc
     1921 aggtttttta tgactaaaaa acgaaattaa tcgtctttcg gtgcgcggtt ggcgcggcga
     1981 cgatcgtttt ccgtcaggtg acgtttacga atacggatag aggtcggagt cacttctacc
     2041 agttcgtcat catcgatgaa ctccagagct tgttccagag tcatgcggat aggcggaacc
     2101 agaacaacag cttcgtcagt accggaagca cgcatgttgg tcagtttctt accggtcagg
     2161 cagtttacag tcaggtcgtt agagcggcta tgaataccga taatctgacc ttcgtaaact
     2221 tctgcaccgt gaccgaggaa cagcttaccg cgatcctgca gaccgaacag cgcgaacgcg
     2281 acagctttac cctgaccgtt agagatcagt acaccgttct gacgctgacc cacttcgccc
     2341 ggacgtacgt cgtcgtagtg gctgaaggtg gagtacagca gaccagtacc ggaagtcatg
     2401 gtcatgaact cagaacggaa gccaatcaga ccacggcttg ggatcacgta gtcgagacgt
     2461 acgcggcctt taccgtctgg attcatgttt ttcaggtcgc ctttacgttc gcccagcgcc
     2521 tgcatcacag aaccctgatg ctgttcttca acgtccagcg tcacgttttc atacggctct
     2581 tgtttacgac cgtcgatttc acggaagatt actttcggac gggataccgc cagttcgaaa
     2641 ccttcacgac gcatgttttc gataagaaca gagaggtgca gttcaccacg accagaaacg
     2701 cggaacgcat cggcgtcttc ggtttcttct acgcgcagcg caacgttgtg taccagttct
     2761 ttgttcagac gatcgaggat ctgacgagac gttacgaact taccttcttt accgcagaac
     2821 ggcgaggtgt taacgcagaa gaacatagaa acggtcggct catcaacgga gagtgccggc
     2881 agcgcttcaa cgttttgcgt gtcgcaaacg gtgtcagaaa tgttcagttc gcccaggccc
     2941 gtgatcgcca cgatatcgcc agcttccgcc agatcggttt cgatacgttc cagaccgagg
     3001 tggcccagca ctttaccgac tttcgcgtta cgggttttgc cttcgctatc gatgatagta
     3061 acctgctggt tcggcttcac tttaccacgc ttgatgcggc caatgccgat aacgccaaca
     3121 tagctgttgt aatcgagctg agaaatctgc atctggaacg gaccgtcaag gtcaacgtcc
     3181 ggcgcaggaa cgtggtcaac aatcgcctga tacagcgggg tcatgtcttc cgccatatct
     3241 tcgtggtcca gacccgcgat accgttcagc gcagaagcgt aaacgatcgg gaagtccagc
     3301 tgctcgtcgg tcgcgtcgag gttaacgaac agatcgaata cctgatccac aacccaatca
     3361 ggacgcgcgc cagggcggtc aactttgttg ataacaacaa tcggcttcag gccgtaagca
     3421 aacgcttttt tggttacgaa gcgcgtttgc ggcatcgggc cgtcaaatgc gtcaaccacc
     3481 agcagcactg agtctaccat ggacattaca cgttcaactt caccaccgaa gtcggcgtgc
     3541 cccggggtat caacgatgtt gatacggtaa tcattccatt tgatagcggt gtttttcgcg
     3601 aggatggtaa tcccacgctc tttctccaaa tcgttggagt ccatcacgcg ctcttgggtt
     3661 tcggcacgag agtcgaacgt accggattgt tggagcagct tgtctaccaa ggtggtttta
     3721 ccatggtcta cgtgcgcgat gatggcgata ttacgcaatt tttcgatcac aactttgcct
     3781 caggcattag aaatagcgcg ttattgtaca cggattaatc gcactacaaa acaggatcac
     3841 aaacatcctc cgcaaacaag tattgcagag tccctttgtg atcgctttca cggagcataa
     3901 aaagggttat ccaaaggtca ttgcaccaat atggtgctta atgtttccat tgaagcacta
     3961 tattggtgca acattcacat catggtgcag cccttttgca cgatgttaag catgataacg
     4021 ccttttaggg gcaatttaaa agttggcaca gatttcgctt tatctttttt acggcgacac
     4081 ggccaaaata attgcagatt tcgttaccac gacgacaatg accaatccag gagagttaaa
     4141 gtatgtccgc tgaacacgta ctgacgatgc tgaacgagca cgaagtgaag tttgttgatt
     4201 tgcgcttcac cgataccaaa ggtaaagaac aacacgtcac tatccctgct catcaggtga
     4261 atgctgaatt cttcgaagaa ggcaaaatgt ttgacggctc ctcgattggc ggctggaaag
     4321 gcattaacga atccgacatg gtgctgatgc cagacgcatc caccgcagtg attgacccgt
     4381 tcttcgccga ctccaccctg attatccgtt gcgacatcct tgaacctggc accctgcaag
     4441 gctatgaccg tgacccgcgc tctattgcga agcgcgccga agattacctg cgttccactg
     4501 gcattgccga caccgtactg ttcgggccag aacctgaatt cttcctgttc gatgacatcc
     4561 gtttcggatc atctatctcc ggttcccacg ttgctatcga cgatatcgaa ggcgcatgga
     4621 actcctccac ccaatacgaa ggtggtaaca aaggtcaccg tccggcagtg aaaggcggtt
     4681 acttcccggt tccgccggta gactctgctc aggatattcg ttctgaaatg tgtctggtga
     4741 tggaacagat gggtctggtg gttgaagccc atcaccacga agtagcgact gctggtcaga
     4801 acgaagtggc tacccgcttc aataccatga ccaaaaaagc tgacgaaatt cagatctaca
     4861 aatatgtcgt gcataacgta gcgcaccgct tcggtaaaac cgcgaccttt atgccaaaac
     4921 cgatgttcgg tgataacggc tccggtatgc actgccacat gtctctgtct aaaaacggcg
     4981 ttaacctgtt cgcaggcgac aaatacgcag gtctgtctga gcaggcgctg tactacattg
     5041 gcggcgtaat caaacacgct aaagcgatta acgccctggc aaacccgacc accaactctt
     5101 ataagcgtct ggtcccgggc tacgaagcac cggtaatgct ggcttactct gcgcgtaacc
     5161 gttctgcgtc tatccgtatt ccggtggttt cttctccgaa agcacgtcgt atcgaagtac
     5221 gtttcccgga tccggcagct aacccgtacc tgtgctttgc tgccctgctg atggccggtc
     5281 ttgatggtat caagaacaag atccatccgg gcgaagccat ggacaaaaac ctgtatgacc
     5341 tgccgccaga agaagcgaaa gagatcccac aggttgcagg ctctctggaa gaagcactga
     5401 acgaactgga tctggaccgc gagttcctga aagccggtgg cgtgttcacg gatgaagcga
     5461 tcgatgcgta catcgctctg cgtcgcgaag aagatgaccg cgtgcgtatg actccgcatc
     5521 cggtagagtt tgagctgtac tacagcgtct aatagttgaa gttgtactac ccggcgcaca
     5581 acgccgggat ttagttgccg tggaaacttt cagcccatct ctgcatgggc ttttttctcc
     5641 gtcaattctc tgatgcttcg cgctttttat ccgtaaaaag ctataatgca ctaaaatggt
     5701 gcaacctgtt caggagactg ctttatggca acaggcacgc agcccgatgc tgggcagatc
     5761 ctcaactcgc tgattaacag tattttgtta attgatgaca atctggcgat ccattacgcc
     5821 aaccccgccg cgcaacaact gctcgcccaa agctcccgca aattgtttgg tacgccgtta
     5881 ccggaactgt tgagctactt ctcattaaat atcgagctga tgcaagaaag tctggaggcg
     5941 gggcaaggct ttaccgataa cgaagtgacg ctggtcatcg atggtcgctc gcatatcctt
     6001 tctgtgacgg cccagcgtat gccggacggc atgatcctgc tggagatggc accgatggat
     6061 aaccagcgcc gcttaagcca ggaacaacta caacatgctc aacaagtagc tgcccgtgat
     6121 ttagtgcgcg gcctggcaca tgagattaaa aatccgcttg gcggtttacg tggcgcggcg
     6181 cagttactca gcaaagcgtt acctgaccca tcactactcg aatataccaa agtgattatc
     6241 gaacaggcgg accggctgcg aaatttggtc gaccgtctgt tggggccgca gctgcccggt
     6301 acgcgcgtta ccgaaagtat tcacaaagtg gctgaacgcg tggtaacgct ggtgtcgatg
     6361 gaactgccgg acaacgtgcg gttgattcgt gattacgatc ccagcctgcc ggaactggcg
     6421 cacgacccgg atcaaattga acaggtcttg ctgaatattg tgcgcaatgc gctacaggcg
     6481 ctggggccgg aaggcggtga aatcattctg cgtacccgca ccgcgtttca actgacctta
     6541 cacggcgagc gctaccggct ggcggcgcgg attgatgtgg aagataacgg gccgggcatt
     6601 ccgcctcatt tgcaggatac gctgttttac ccgatggtca gcggccgcga aggtggcacc
     6661 gggcttggct tatccatcgc ccgtaatttg attgatcagc attcaggcaa aattgaattt
     6721 accagttggc caggtcatac cgagttctcg gtttacctgc ctatcaggaa ataaaggtga
     6781 cgtttatgca acgagggata gtctgggtag tcgatgacga tagttccatc cgttgggtgc
     6841 ttgaacgtgc gctcgctgga gcgggtttaa cctgtacgac atttgagaac ggcgcggaag
     6901 tactggaggc gctggcgagc aaaacgccgg atgtgctgct ttcagatatc cgtatgccgg
     6961 gaatggacgg gctggcgctg ctcaagcaga ttaaacagcg ccatccaatg cttccggtca
     7021 tcattatgac cgcacattcc gatctggatg ctgccgtcag cgcctatcaa caaggggcgt
     7081 ttgattatct gcccaaaccg tttgatatcg acgaagcagt ggcgctggtt gagcgcgcta
     7141 tcagtcatta ccaggaacag cagcagccgc gtaatattca gcttaacggc ccaacgaccg
     7201 atatcatcgg cgaagcgcaa gccatgcagg acgtgttccg tattatcggt cggctttcgc
     7261 gttcttctat tagcgtgctg attaacggcg aatccggcac cggtaaagaa ctggtcgctc
     7321 atgccctgca tcgccacagt ccgcgcacca aagcgccgtt tatcgcgctg aatatggcag
     7381 ctatcccaaa agatttgatc gaatcagaac tgtttggcca cgagaaaggc gcgtttactg
     7441 gcgcgaatac cattcgtcag gggcgttttg aacaggccga tggcggcacg ttatttctcg
     7501 acgaaattgg agatatgccg ctggatgtgc agacgcgttt gctgcgcgtg ctggcagacg
     7561 gtcagtttta tcgcgttggc ggctatgcgc cggtgaaagt ggatgtgcgg attatcgctg
     7621 ccactcacca gaatcttgaa cagcgggtgc aggaaggtaa gttccgtgag gatctgttcc
     7681 accgcctgaa cgttatccgc gttcatctgc cgccgctgcg cgaacgtcgg gaagatattc
     7741 cccgtctggc gcgccatttt ttacaggttg ccgcgcgcga actgggcgta gaagcgaagt
     7801 tgctgcatcc ggaaaccgaa gctgctctga cgcgtctggc gtggccaggc aacgtgcgcc
     7861 agctggaaaa cacctgccgc tggctaacgg tgatggccgc cgggcaggaa gtgttgattc
     7921 aggatttgcc cggcgaactg tttgaatcaa cggttgcgga gagtacttcg caaatgcaac
     7981 cggacagttg ggcgacactt ttagcgcagt gggcagacag agcgctgcgt tccggtcatc
     8041 aaaatctgct ttccgaagcg cagccagagc tggagcggac gttactgacg accgcgttgc
     8101 gacatacgca ggggcataaa caggaagcgg cgcggctact cggctggggc cgcaacactc
     8161 tgacgcgtaa gttaaaagag ctggggatgg agtgattcac ggcttgtgtg taaagattga
     8221 ttattgagcg caaattgctg gtattttgcg ctttactgtt ccgataagtt cagtatgatc
     8281 ttgcccggaa aacggggaga gtcattatgc tggaatcaat aattaatctg gtatcgagtg
     8341 gcgcagttga cagccacaca ccgcaaactg ccgttgctgc cgtgctgtgt gccgcgatga
     8401 ttgggctgtt tagctgaagc tcgttgttta tgccggatgc ggagaaagtc gtaggcctga
     8461 taagacgcga ccagcgtcgc atcaggcatc atgctcagat gcctgatgcg gcgtaaacgc
     8521 cttatccggc ctacaaaagc aataatttca acaggttttg ttgcccagcg catcaggcaa
     8581 tcttgttctc agcacattga tggcgctttc gttttctact ttgtaaacga agcgccattc
     8641 actctttaaa tcacccgtga gaactgctgc atccgcgctt tctggcgcag ataggtatca
     8701 aagcacatgc aaatgttgcg gatcagcaag cgacctttcg ccgtcacctg tattcccttc
     8761 tcatccacat ccaccagccc atcttttgct aacggggcga gcagcttgag atcttccgca
     8821 aagtaatcag cgaagtgcaa atcccactgt ttctcaagag gggcgtaatc cagacggaag
     8881 ttgcagatga gcgacttaat cacatcgcgg cgaatacagt catcacgcgt tagcgcaata
     8941 ccacgccaca gcgcattgcc ttgttcatcc acttgctgat agtactgctt caactctttc
     9001 tggttctgcg cgtagcagtc gccaatcatg ctgatggcgg aaacgcccat ccccagcaga
     9061 tcggtatcgc cctgagtggt gtagccctgg aagttacgat gcagcacgcc ttcacgctgg
     9121 gccaccgcca gctcgtcatc aggacgggca aagtgatcca taccgataaa ctgatagccc
     9181 gattgcgtca ggaaggcgat ggtttcctgc aggatatcga gtttttgctg cggactcggc
     9241 aggtcagcat ctttgatttt gcgctgagca gcaaaaatgg tcggcagatg cgcgtagtta
     9301 aaaacgctca gacgatcggg gttcagctcc gccacacgtt tcagggtaaa ggcgaaactc
     9361 tccggcgtct gtttcggcag gccgtaaatc aggtcgatgt tggtggaggt aaaaccaatc
     9421 tcacgcgcat ggttaagcag tgcaaagatg aactcttcat cctgctcgcg gttaaccaga
     9481 cgttgcactt ctttgttgaa gtcctgcacg cccatgctca ggcgattaaa gccttcggcg
     9541 cgtaaatgat cgagtacatc cagttcgatt tcccgcggat cgacttcgat cgaaatctcc
     9601 gcatcggtat tgaaatggaa gttttcgcgc agcagcttca tcagacggct gatttgcgct
     9661 ttattcagat acgtcggcgt tccgccgccc cagtgcaatt ggctgacgtg acgcccggcg
     9721 aacagcggtg cacgatggac gatttcttgc tccagcgcgt ccagatactg atcggccttg
     9781 tgctgctggc gagtaacaat cttattgcaa ccgcagaagt aacaaagctt atggcagaac
     9841 gggatatgta cgtagagaga taatggacgc tcaggatagc gcgccacggc ttgtaaaaac
     9901 gcctgttcgc cgaagtcttc tgaaaactcc agcgcggtcg ggtacgaggt gtatcgtggc
     9961 ccggaatagt tatatttctg gatcagggcc agatcccagt cgatttgctg tacagacatg
    10021 ctcactcctt ccgatggcgt ctctggcggc tacggcgtac cggcggatgc ccgtttcggg
    10081 tcatcagccg gttgcgcagc cacttttgtc gccgcgacaa ccgctgtagt ttaacgaata
    10141 accacaccag ataacatata accggaaggg ttatcagcag aacgggaagg cccacggcgg
    10201 caatccgtta gttgccccgc agcagacgca tcatgtcttc ttgcttctcg tcttcttcct
    10261 cttcttcgtc atcgtcataa gagaggccga gtttctgcat caactcatca atacgatcca
    10321 gtttagcatc cacccaggat tgctcttcgg cgctcagggt ttcgcctgct tccagacgtt
    10381 ccagcagcgc atccagacgc tcatccgttt ccagtaattc caactccgcc tgcggtgaaa
    10441 gcataggttt ctcactcttc ggtttgtgct gtttggtgac tttttcagtc actcccaatg
    10501 gaataggggt tttactgcca atacgtggat cttttggtgc gttctggcct ttgctgcctg
    10561 acgtggtgtt tccgcccgct gcgcggctgc ccggcgcatg accacgacgt tttttctgac
    10621 gcttacggtc acgagcttcc tgatccagct cctcgcgtgt ttttcgacgc gcttttgcat
    10681 gacctttgct gcgtgagttt gaagatgatg gtttcataat ggttcgtctt ttagccgttt
    10741 tattccgtat agatttgcgg cggaatctaa cagaaagcaa gcaaagaaaa aaggcgacag
    10801 attactctgt cgcctttttt cctgactcat aacccttaac gggtcttaca atccttgcag
    10861 gccgccaaca caccctgttt ttccattagc cggaacaatc cgtgttgatt ccatttccgt
    10921 ttaattacgt ctccggacgt ttgtcttcct gacaatcctc tgtcttcgcc tcctggcgct
    10981 cctgaccctc atcctgagtc attgttcctg ttagcgtcct cgctctatgg gtgctacttt
    11041 acgcctttgc tttaaacaaa caatcaaccg ccatcagtat ttgcacattt tcagagtttt
    11101 atataaaagc aattcactga tttatatagc ttatttgaca tgcatcgcag aattttctct
    11161 gctatttcgc ataaagacta tcggcaatgt ctcacaaagc gtatggcttt tacttacaac
    11221 gctcacgccg aaaaatccgc cttttattat ggttcaggta taatcgccac aaagctttga
    11281 ctgacggaga cgaccgcttt gactaatttg aattatcaac agacgcattt tgtgatgagt
    11341 gcgcctgata ttcgccacct accttccgat accggaattg aagtggcttt tgcaggccgt
    11401 tccaacgcag gtaaatccag cgcgctgaac acgctgacta accagaaaag cctggctcgt
    11461 acctcaaaaa ccccagggcg cacccagctt atcaacctgt ttgaagtggc tgacggcaag
    11521 cgtctggttg acttgcctgg gtacggttat gcggaagtcc cggaagagat gaagcgcaaa
    11581 tggcagcgtg cgctcggcga atacctcgaa aaacgtcaga gcctgcaagg tctggtggtg
    11641 ctaatggata ttcgccatcc gctgaaagat ttggatcagc agatgattga gtgggcggta
    11701 gacagcaata tcgccgttct ggtgctgctg accaaagcgg acaaactggc aagcggcgca
    11761 cgtaaagcgc tattgaatat ggtgcgtgaa gctgtactgg cgtttaacgg tgatgtgcag
    11821 gttgaaacgt tttcttcgtt gaagaaacaa ggcgtagaca agctgcggca gaaactggat
    11881 acctggttta gcgagatgca gcctgtagaa gaaacgcagg acggcgaata attttcttgc
    11941 cttaatgctt gtgccggatg tggcgtatcc ggcccgtaaa ttcaatagat tacaattcta
    12001 ccgttggcct gataaacata gcgcatcagg cattacggat cttttctttc gcccaataaa
    12061 aaacgcccca gtcattactg actggggcgg ctaaaatatt cagccaaatc cgattacgtg
    12121 aagtaaaagg tctgaaagat agaacatctt acctctgtac cctacgcgac taactttact
    12181 cttttttgtt cagttcagaa agcacttttt gtaatttttt ttcagttgtt gcataggaat
    12241 ttctaatagc catcacaaaa cgtgacagct tatgttgctt acttacgaaa aaagggcatg
    12301 ttcaggcgaa tcttagtgcg cctgatccca gttttcgcca ctccccactt ccaccagcaa
    12361 cggcacatcc agacgggtac agttttccat cagttgatga atctgcttcg cgacggcatc
    12421 aacatcatct ttatgaactt caaataccag ttcatcgtgt acctgcatga tcatacgtac
    12481 acgcggttgc tcagcctgta accacgcatc aacggcaatc atcgcccgtt tgataatgtc
    12541 ggcggcggtt ccctgcattg gcgcgttaat ggctgcacgt tcagccgctg cacgacgagc
    12601 accattgctg gatttgatat ccggcagata cagacggcgt ccgtccagcg tttcaacgta
    12661 gcccagctct ttcgcctgag cacgggtgcg ttccatatac tccagcacgc cagggtagcg
    12721 ttcgaagtaa aggtccatgt acttctgcgc ttctttacgt ggaatgttca attgccgcgc
    12781 cagaccgaaa gcactcatgc cataaatcag accaaagttg atcgctttcg cgctacggcg
    12841 ttgctcgctg gtgacggttt ccagtggcaa accaaacact tctgccgccg ttgcccggtg
    12901 gatatctttt ccttccgcga atgcggtcag caagccttta tcacgcgaga gatgcgccat
    12961 aatgcgcagt tcaatctgcg agtagtccgc tgagacaatc acataatcct ctggcgcaat
    13021 aaacgcctgg cggatacgac gaccttcttc gttacgcacc ggaatgtttt gcaggttagg
    13081 atcggttgac gataaacgtc ccgttgcagt tactgcctgg tgataagagg tatgcacacg
    13141 cccggttttc gggttgatca tcagcggcag cttgtcggtg taagtcgact tcagcttcgc
    13201 cagaccacga tactccagaa tcacttttgg caacggatag tccagcgcca gttcttccag
    13261 tacctcttcc gacgttgacg gcgcgccacc cggcgttttc ttcagcggtt taataccctg
    13321 tttttcaaac agaatggttt gcaactgctt ggtggaagaa aggttaaatt cttcacctgc
    13381 aatttcatgc gctttctttt ccagctcagc cagacgcaag gtgagctctt cagaatgatt
    13441 gtgcagcact ttcggatcga tcttcacacc gttacgttca atgcgtgaaa gcaccggcac
    13501 cagcggcatt tcgatattct cgaagacgtt caacggccct ttgtgttttt gcagatccgg
    13561 ccacattttc agatgcaact gcaaggtgac atctgcatct tcggcggcgt aacgtccggc
    13621 ctcttcgagg gcaatctggt taaaggtcag ttgatttttg cctttacccg caatctcttc
    13681 aaaagtgatg gttttgtgct tcaaccaacg ttccgcgagg ctgtccatat cgtgacgccc
    13741 ggcaacgcta ttgagaatgt aggactccag catggtatca aacgcaatcc cacgcagttc
    13801 aatgccgtaa ttcgccagaa taccgcgatc gtatttcagg ttttgcccga ccttcagcgc
    13861 cttttcatct tccagcagcg gttttagcaa ctcgagtgca cgctcgtgag agatttgatc
    13921 gggcgcatca agataatcat gagcaaccgg aatatatgcc gctacgcctg gctcgatagc
    13981 aaaagaaagc ccgaccaggt tagcagagat gttatcaagg ctgtcggttt cggtatcaaa
    14041 tgcaaatacc ggcgcttttt ccagcttcgc aatccacgct ttcagtgttt cttcatcaag
    14101 gatagtgacg tagttgtcat aagaaatcac cgttgccgtc acttctggcg cttcgtctgc
    14161 aacactggtt tcctgcggct tcgcggctgg ttttgcccct ttggcctgta accatttgcc
    14221 cgcttcgaca tcagcagtcc agcgtttgaa ctcatacttt ttgaacagcc ccaacaactc
    14281 ttccgctgcc ggttgctgca cttccagttg ttcacaggtc agctccagtt caacatcggt
    14341 tttaatcgtc gccaactggt atgagagata agcaacttct ttgttttgct cgagcttcgc
    14401 tgccattgtt ttcgcgccac ggaagctcaa cccagcaatt ttttctggct cggcatacag
    14461 cgtatccagc ccgccaagac cttgcagcaa tgcctgcgcg gttttttcac cgacgcccgg
    14521 tacgccagga atgttatcag aggagtcacc catcagcgcc aggaagtcga tgatcaattc
    14581 tggcggcacg ccgtacttat tcaccacctc ttccggtccg aggatggtat tcgtcatggt
    14641 attgataagc gtaatatttg gcgtcaccag ctgcgccata tctttatcgc cagtgctaat
    14701 cagcaccgga cgcccggctt tttcggcttc gcgcgccaga gtaccgataa cgtcgtccgc
    14761 ttctacgcca gaaaccgcca gcagcggcag tcccatcgct ttaaccatcg cgtgcagagg
    14821 ttcgatttgt gctcgcagat cgtccggcat tggcgggcga tgtgatttgt aatgttcaaa
    14881 cagttcatca cgaaaggttt ttcccttggc gtcaaagacc accgctgcat gcgtcggttt
    14941 atattgcatg atcagactgc gcagcatgtt gaggacacca tacatcgcac cggtcggctc
    15001 gcctgcgctg ttagtcagcg ggggaaacgc gtgatatgcg cgataaagat aagatgaacc
    15061 atctacaagg ataagtggat tttgggggat ctgaaccata atgtccgtgc ctgtttatca
    15121 gattatgggt aaaggatgcc acagacagga tgaaaacatg agtttttcac cgcttttcgt
    15181 gaaaatcttt tgtagatctg ctggatcgct cgcaaatcgt catgtggata actttgtgca
    15241 taaattattt acatcgcttt taatttatta tcatgctttt tacagcatga tgtttgtctt
    15301 tatttttcat gcagttaaaa ataaatccat ccctaataat tgagataatg ggattttttc
    15361 taatgtggat agatatgaat tatttttctc cttaaggatc atccgttatt tgggtcattt
    15421 tctgctaaaa tctgcgcctt gcgcttcttt agtgcactgt ctatggctaa ccttttgaat
    15481 aaattcatta tgacgagaat actcgctgcg ataacccttt tgctgagtat cgtattgacc
    15541 attttggtca ctattttttg ttccgtcccg atcatcattg ccgggattgt aaaactattg
    15601 ctacctgtgc cagtcatttg gcgaaaggtt tcgcgttttt gtgattttat gatgtattgc
    15661 tggtgtgaag gtctggcggt attactgcat ctgaacccac acctgcaatg ggaagttcac
    15721 gggctggaag ggttaagtaa gaagaactgg tatctgctta tttgtaacca ccgtagttgg
    15781 gcagatattg tcgtactgtg cgtgctgttt cgtaagcaca ttccgatgaa taaatatttc
    15841 ctcaagcaac agctggcctg ggtgccattt cttggcctgg cgtgctgggc gctggatatg
    15901 ccgtttatga agcgttattc ccgcgcttat ttattacgtc atccggagcg acgtggtcaa
    15961 gatgttgaaa ccactcggcg ttcttgtgaa aagtttcgcc tgcatcccac caccattgtc
    16021 aatttcgttg aaggctcccg tttcacgcaa gaaaaacatc agcaaaccca ctccactttt
    16081 caaaacttgt tgccaccaaa ggcggcaggc attgcgatgg cgctaaatgt actgggtaaa
    16141 caattcgata aactgcttaa tgttacgctg tgttatccgg acaataaccg tcagccgttc
    16201 ttcgatatgt taagcggtaa actgacgcgg attgtcgtac atgtggattt acagcctatt
    16261 gccgatgagt tacatggcga ctacatcaat gacaaaagct ttaagcgcca ttttcagcaa
    16321 tggctgaact cattgtggca ggaaaaagac cggcttctga cgtctctcat atcatcacag
    16381 cgtcagaata agtaagtttt actcgcctct gaatgagcag aggcgagtga gtattttaat
    16441 gaacaaaacg tccggcacga gacataaatt cttcttcgct catctcctgt ccgttaaaaa
    16501 caactttacc cggtgtataa cgcagctgca gcgaggcggt attgttgtca atcgtgatta
    16561 actgaaacat ctgacctaat gtcatcatcc cgctgatttg tttatcagcc tgcttttgcg
    16621 ctttttccgc atccattcct tcagataaat taagctgttt tgcggtttct gtgacgacat
    16681 taagcggtaa cttcacatca aaattgagcg atttgatatc tttgtttgtg gatgatgaag
    16741 atttggctgg gtcggcaata ctgatatcca gattggcatt tagttcgccg agtgcgttct
    16801 tccatcttac cggttgttta atggtcggct cacttttttg taataacggt aaatactctt
    16861 tgaacaatgc agcattcact tcttgcagag caacttcatc gtttgctaac tcagggtgtg
    16921 cagcaagctg cttctgcatc gcaatgttat actggatgat aaattggcgc actgctgagg
    16981 gatcaataga ttcagcaatc aatgaccact caccactacc gagttgctga ttcccttttg
    17041 ttaacttatc aagttcgtag gtcagattga cattgatata atctttgtct ttaacgcgat
    17101 ccagcctggt ttttgcaacg aaagcatcga tttgggcaac gtcttccccg tgattgataa
    17161 tgttaatctg attaaatttc gactcgcttt caccaaccgg aaatttcttt tcttctaatc
    17221 gcgccagaga atcaatggta aaactttttg ccgttacttt gttcattggt gataattgca
    17281 gaacaatatc ttcgactttg ccttcaatat ccaggttaga caatgaacta ccatcaccat
    17341 taaaagtaaa ctggccctca ccaaaagaaa actttccata ctctgcaggg gcaacatcta
    17401 atgtcgtaga gaatgagcca ccaaacgcga atcgcaatgt cgcttccacg ggcgatttat
    17461 ttttcgcggc gataaatagc ggttgtgtca gttcattatt cactacgttc acttttgccg
    17521 ctgccagtgc tgggatgaca ttaccatgca ttaacattgt gatgggcaac ggaccgtgct
    17581 caacatccac atcaaataca actttctggc ctggcttgat attgagatcg ggtgcaccgt
    17641 tatcgaatgt catttgcatt tgaatacggg aattaaaaaa gcctttgtca aagtttttat
    17701 aacttaacgt catatcgtag gcatgttcac ctttcttttt cgcatcgtta aaatctttaa
    17761 taaacttttc gataccaggc tgaatttgcg tacctgtgta ccatgtgcca ccaccccaga
    17821 ttacggctaa cgcaacaata acacctgtag ctgacttacg tatcatataa atattccctg
    17881 ataaaacagc aaaactttga atatccactt atgctgaaat aatgtccagc ggcaggatgc
    17941 attatcagaa tgtatttata tttattaaat aataaaaaaa gcccgtgaat attcacgggc
    18001 tttatgtaat ttacattgaa ttattttttc tcggacagat atttcaccgt atcagcatac
    18061 tgctgaacaa aaacatccat attgctggta tccataccct gcggattcag ctgatattta
    18121 ccgttaacaa acatcgccgg aacaccacgc aattgcacat cagctgcggc tttttcctgc
    18181 tgagcgacca gagatttcac cacgaagctg ttccacgccg cgtcgtactc ttcaccttta
    18241 atacctgcat tgataaatac atcgcggata tcagatgctg aacgaatggt ctgggttttc
    18301 tgtacgcctt caaacagcgg aacagtcact ttgtcttcca cgcccagcgc catcgccaca
    18361 gcccatgcct gagtcagatc tttgcccagg tcaccaccca tgaagttgac gtggtattta
    18421 gtcatcttca cgccttccgg cagttttttc ttcacattat cagaaatatg cagaacttct
    18481 tcaaactgat agcagtgcgg gcagaagaaa gagaaaaact ccagcacttg cggcgcgcca
    18541 gctaccggtt tttccagggt agtgtactgt ttaccatctt catactgcgc cgccgatgca
    18601 ctaaacgcta aaactaaacc agccagcgcc agccaaatct ttttcatgat ctactctctc
    18661 cgattaatac ataggtgtta attgcaaagg ggattcttgt agaacttttg cctgttctat
    18721 aaaagtcgcc gtctgtcgta gccagtaatc ttccccggtt aaccacggga aatttttcgg
    18781 gaacgcggga tcagcccaat gccgcattag ccaggcaaga taataaacca aacgcatggc
    18841 gcgtaaaggt tcaatcagtc cgatttcagc ggtgtcgaac tcgctaaatt cttcataagc
    18901 ttcaataata gtttccagtt gcatccgctg ctgggcttta tcgccattga gcaacatcca
    18961 caaatcctga atagctggac cattacgtgc atcatccaga tcaacaaaca ttggaccatc
    19021 gcgccagaga atattcccgg cgtggcagtc tccatgtagc cgcagaacgg tgaaatcttc
    19081 ccgccagtgt gctgtaacgg cggcgatcag ctcatctgtc gctttcagaa atgccgcttt
    19141 caacccggaa ggtatcagtg tagcgtcctc aaacagcttg cgaggctcaa tgagatattc
    19201 attcagaccg atggtcgggc gatgaataaa aagctgtttg cgccccgtct ggtgcatacg
    19261 ccctaaataa cgcccaaccg cttccatctg atcgatatta tcagcttcga actggcgacc
    19321 accgacgctt ggaaaaacag cgaaataaaa tccctgatga ttcaataaag tctgaccgtt
    19381 aaaggccaca ggtgctgcga ccggaacttc atcatttacc agctgcaacg caaattgatg
    19441 ttcttcgagg atttgatcgg ctgtccaacg ttcagggcga taaaatttga cgacaaaacg
    19501 tcgacgctct tcgtcctgaa attgatagac acggttttca tagctgttaa gcggggtaag
    19561 accggaatcc acccggatcc cttgctcaaa cagagcgtcc atgatggtat ccgggtgtag
    19621 tgtctggaaa gtaaaagcgc tgttgttcat cccatcatcc ggaaaacatt acgaatgatg
    19681 aaggatatca tttcacgatg attttggtgg cgccgcttac aagcttttac tctttaatta
    19741 cgccgcgagc gcgtaacagc gccgttttga agtcttcttc ataatctttc tgcaaacccg
    19801 ggatcaccgc atctttcgcg gaatcacgca ttttcaggtg atagatcaga atgtcatccg
    19861 tcaaatccgc cagttcgccg tcaaaacctg actctttcgc cagtttctgc aaaaattgca
    19921 acaagttaaa gtctggctct ttttgccagg ctggctggag gagttcaata acttcattca
    19981 gacgtttaca tttcatagta atgctcctta ctcttgagac agacacgtta gcagggtcaa
    20041 tcccacaata aaagaggcga tatcggtgaa tctgatgacg acgataacag gcgttgtgct
    20101 ggcaggcggt aaagccagac gaatgggcgg cgtagataaa ggattgctgg acttaaatgg
    20161 caaaccgctg tggcaacatg tcgctgacgc gcttaagacg cagctctctc acgtcgtggt
    20221 taatgctaat cgtcatcagg aaatctatca ggcaggcggt ctgaaagtga ttgaagattc
    20281 actggcggat tacccaggcc ctctggcagg aatgctttca gtaatgcagc aggaagctgg
    20341 tgagtggttt ttgttttgtc cgtgcgatac gccttacatt ccccatgatt tagccgcccg
    20401 gcttaatcat cagcgcaaag atgcgcctgt cgtgtgggtc catgacggtg aacgcgatca
    20461 cccgactatt gctctggtaa accgcgctat tgagccttta ttactggaat atctgcaagc
    20521 aggagaacgc cgggtaatgg catttatgcg tctggctggc ggtcatgcgg ttgatttcag
    20581 cgatcataaa gatgcatttg ttaacgtgaa tacgccagag gagcttgccc gatggcagga
    20641 aaaacgatga taccgttact cgcctttgcc gcgtggagtg gtaccggaaa aactacgctt
    20701 ttgaaaaaac tgatcccggc attatgcgcc agagggatcc gtccggggct gattaagcat
    20761 acgcaccatg atatggatgt tgataagcca ggcaaagata gctatgagct gcgcaaggct
    20821 ggcgcggcac aaaccatcgt tgccagccag caacgatggg ccttgatgac ggaaacacca
    20881 gacgaagaag agctggatct acattttctc gcaagtcgaa tggatacctc aaagctggat
    20941 ttgattctgg tcgaagggtt taagcatgaa gagatcgcaa agattgtgct gtttcgcgat
    21001 gcagccggac atcgacctga agaattagtg atagacaggc atgttattgc tgtagccagt
    21061 gatgtgccgc ttaatcttga tgtcgcatta ctggatatta atgatgttga ggggctggct
    21121 gattttgtgg tggagtggat gcaaaagcag aacggataag caagagtagg ctggatcaga
    21181 cgcagccagt gtcgcatccg acctttttct gcaccagcgc aaaaaggccc gactgggcct
    21241 tctgcttaat ttga
//
LOCUS       DAETPZ010000065        21091 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.065, whole genome shotgun sequence.
ACCESSION   DAETPZ010000065 DAETPZ010000000
VERSION     DAETPZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.065"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..82
                     /locus_tag="K0W59_002748"
     CDS             <1..82
                     /locus_tag="K0W59_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3023722.1"
                     /translation="KSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(76..3195)
                     /locus_tag="K0W59_002749"
     CDS             complement(76..3195)
                     /locus_tag="K0W59_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HBI3023723.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLCMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVQVMTPTY"
     gene            complement(3202..3375)
                     /locus_tag="K0W59_002750"
     CDS             complement(3202..3375)
                     /locus_tag="K0W59_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023724.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            3493..4686
                     /locus_tag="K0W59_002751"
     CDS             3493..4686
                     /locus_tag="K0W59_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBI3023725.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLAQAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKTTQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4884..6137)
                     /gene="glyA"
                     /locus_tag="K0W59_002752"
     CDS             complement(4884..6137)
                     /gene="glyA"
                     /locus_tag="K0W59_002752"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBI3023726.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6464..7654
                     /gene="hmpA"
                     /locus_tag="K0W59_002753"
     CDS             6464..7654
                     /gene="hmpA"
                     /locus_tag="K0W59_002753"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HBI3023727.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(7699..8037)
                     /gene="glnB"
                     /locus_tag="K0W59_002754"
     CDS             complement(7699..8037)
                     /gene="glnB"
                     /locus_tag="K0W59_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HBI3023728.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(8098..9432)
                     /gene="glrR"
                     /locus_tag="K0W59_002755"
     CDS             complement(8098..9432)
                     /gene="glrR"
                     /locus_tag="K0W59_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HBI3023729.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9422..10135)
                     /gene="qseG"
                     /locus_tag="K0W59_002756"
     CDS             complement(9422..10135)
                     /gene="qseG"
                     /locus_tag="K0W59_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HBI3023730.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10300..11727)
                     /gene="qseE"
                     /locus_tag="K0W59_002757"
     CDS             complement(10300..11727)
                     /gene="qseE"
                     /locus_tag="K0W59_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HBI3023731.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12285..16172)
                     /gene="purL"
                     /locus_tag="K0W59_002758"
                     /gene_synonym="purI"
     CDS             complement(12285..16172)
                     /gene="purL"
                     /locus_tag="K0W59_002758"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBI3023732.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16430..17986
                     /gene="mltF"
                     /locus_tag="K0W59_002759"
     CDS             16430..17986
                     /gene="mltF"
                     /locus_tag="K0W59_002759"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HBI3023733.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(17983..18519)
                     /gene="tadA"
                     /locus_tag="K0W59_002760"
     CDS             complement(17983..18519)
                     /gene="tadA"
                     /locus_tag="K0W59_002760"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBI3023734.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(18544..19179)
                     /gene="yfhb"
                     /locus_tag="K0W59_002761"
     CDS             complement(18544..19179)
                     /gene="yfhb"
                     /locus_tag="K0W59_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HBI3023735.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19388..20236
                     /locus_tag="K0W59_002762"
     CDS             19388..20236
                     /locus_tag="K0W59_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBI3023736.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVVQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(20575..21091)
                     /locus_tag="K0W59_002763"
                     /pseudo
     CDS             complement(20575..21091)
                     /locus_tag="K0W59_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         5072 a         5694 c         5367 g         4958 t
ORIGIN      
        1 gaagtcactg tcgttctcaa aatcggtgga gctgcatgac aaggtcatcg ggcattatct
       61 gaacataaaa cactatcagt aagttggagt cattacctga acgtagttaa aaataaaagg
      121 catcacagtg gtgaataatg ccgctttcgg ccaggccgga ttaatcgccg tcaattcagc
      181 gcgagcagac tcatattgcg caacctcccc gagtcccgcg gacgccagca tcgccaccat
      241 caggcatttt tctttatgct gctgttgtaa atcgccatac agcgataaca ggtcaggttg
      301 tgaaacagca aagaaatccg cctcaatact ggttttcgcc atctcttgcg cccactgttt
      361 catttcacta aacagttgct gtgcggtttg ctgttcaccc agcagtcgca gcgccattcc
      421 ttgccagaag agataatcaa ccggctgatc gttgtaataa ctgtggatgt taatggtgcg
      481 atcgccggtc gccgccagac gtaaacaacg catcgcttca gtttcatcgc cctgcgcgtt
      541 ggcgcatatc gcctgccaga accagatgtc gttatcagtt tgccccggta aacggccttc
      601 gcttaaattc tccggataat gcagcgcggc atgaagcagt tcgctggcct gctgcggctc
      661 tctggcatca agatgctgcc aggcgcgtaa taactggttg aggataaact gactggtgac
      721 cttcccttcc ccgccttccc acgggtggaa tttacgcgtg gcgagaatgt ccgccgcttt
      781 gtctgcctga cccgtgagat gccacaaatt gagcagttct gcggtcatgt cgtcgcgttt
      841 cagcgcaatt tccagattat tttccagacg cgccagtcgt ttctccggtg tggctccact
      901 taacttatcc agcaaatccc gttcgaaaag cagacgtgca tcctgcggcg caagctgata
      961 agcattatca agataacgcg cggccagctc ataatcgtgt ttcttattcc acgcatggat
     1021 cgctaacccg cgccagccgt cggcaaactc cggcgacatc tctacgcaac gttgccataa
     1081 ggcaatggct ttgttgtagc tacgtttgtt gtagtagaag caggccagta aatggcgagc
     1141 aaaccagcac tcttcaatac tctccagcgc cgccacttct tccagcgtat tcgggaaacg
     1201 gacaaactgc gggaagacat caatggcttt tgcgaccagt tcgccacgtt cggctttcgg
     1261 cagcaagctg gcttgcaggt aaagcggcag cgtgcgctgg cagtccagtg cgttcagcat
     1321 ctctgccgcc aaggtgggca ttccccagtt aatcagttgc ccggcggtca tcagagcgtt
     1381 aacgtcgcgt ccctgacaca gaccgcgcca ctgcgcgagg gctgattcgc tacgaccatc
     1441 gaaccagttc agccaccaca gagtggcgtt cagcggataa tcacgcagca gtttctcgcg
     1501 ctgcaaacgc gcgttgtcct gacgaccact taacaccagc agcagattat gcaagcaaag
     1561 cacttcctga ttggttgggc aggcacgaag actttgttgg caaaaatcca gacccgcgtc
     1621 gaagttatca ttacgcgccg ccagtcgtgc cagaccataa tagccgcctg ctttactatt
     1681 gccgctccag accgcacgcc agaaatcctc ttcggcttgt tgatattgtc cctgacgttc
     1741 gtaagcactg gcgcgaatca aacttgcttg tccacactgc gggtttttgt tcagcgcatg
     1801 tgcgcgtttc agcgcctgac tggcatacgc caccgcttgc gggaaatctg cgcggttata
     1861 ttccagcatc gccagcgcca ggttacagcg ataatccagc gggtccagcg ccacgccgcg
     1921 caggtagtaa tcgaacggtg aacggctggc gtgatgatat tgctccagat gctgaccgat
     1981 aaaccaggct tcatctgtac tggtaatgtc ttgtgctgcc agtggcgctt tggcgacgtc
     2041 cggcaacggc aactcctgcg gctgatgttc ctgataactc agtacaatat tgccgtcggc
     2101 atcggagagt tcaatggtca gtctgtcagg attgataccg tgcaacacgc cctggatggc
     2161 ggtcgctggt gtcagggcca cggcatcatc gagcaacgcg ttgcatttgc cgatttcgcg
     2221 gatcgccaga cggtatccgt tcaacggaga gatggcatac agcccccact caatcccccg
     2281 ctcactacgc tggagtttta tcaccgcatc gcgggaggca ttttgcacca tgcacagaga
     2341 atgataaggc aggaaatact gctcgaaacg cttctcttcg taagcatcaa gccaggtaaa
     2401 atcaggctgg ttatcggcaa aaataccggt catcagttcg atatacgggc cgttattgtc
     2461 ggtcagactc ttatcccacg cctggccaaa ttcactgtgt ccccaactcc actgtttttt
     2521 acctggcgca atatggtggt tggcaacgtg cagcaaaccg ccatcttcat cgtgacacca
     2581 cgcgccgaca aaatcgtact gcgatttttc ggccatatat gaggttggaa caggcacatt
     2641 tttatagcga gaaatgtcca ctccggcgga gtagtccact ttgtagtaag tgccggtggc
     2701 gatggggaaa gcggagacgg cccgtttgcc gtgatcaaac accgcagtca catccggtgg
     2761 gaaaacgctc tgatgccctt cacccccttt cactgccggg ttggcccacc acaagaaatg
     2821 acgcggcgtg gcgttgccgt tatagacgcg gctggcgatt tccagtgccg cccggtcagg
     2881 gcgcagggtg aaacctgtca tcacctgtaa accatgcatc ggctccgttt cgccgaccca
     2941 caccgtctgt gcaccgtctt catgggcttc gagggtgaaa tcaacgggca taaaggtggt
     3001 cgggcgatgg tgttgcggcc agttaaactc aatcccgccg gagatccacg gtcccagcag
     3061 ccccaccagc gcaggtttaa tgacttcatt gtgatagaca aaatcgcgct gtttcacttt
     3121 atcccatgcg cgatgcaccc gaccgcccag ttccggcagg atcatcactt tgatgtagtc
     3181 gttttccagc cacaccgcct gctaggattt cagggttttc tgctcgctca gcgtatcggt
     3241 cacgccgtag ggataaaccg cgccggacga tccctgataa acgcgatttt ccaggaacat
     3301 gggatgtata tcctgcggcc cggtttcata ggtcgggatc tcaacgcgct cttgccacac
     3361 ttttactgga gtcatggtgc cctcaatatt aacaagacat actgaattaa aagatttgtg
     3421 gcagtgtatt gaacaatctg gcaatgtttt cgcggaataa tcacgcaatt aactaaacaa
     3481 ggtttagtga agatgagagc ctgcattaac aatcaacaga ttcgccacca taacaaatgc
     3541 gtgattctgg aactgctgta ccggcaaaag cgcgccaata aatcaacgct ggcccggctg
     3601 gcgcaaattt cgattccggc agtcagtaat attttgcagg aactggaaag cgaaaaacgg
     3661 gtggtgaata ttgacgatga aagccagacg cgcgggcata gtagcggtac atggctgatt
     3721 gcgccggaag gtgactggac gctgtgcctg aacgtgacgc ccaccagtat tgagtgtcag
     3781 gttgctaatg cttgtttaag tccgaaaggc gaatttgagt atttacagat tgatgtaccg
     3841 acgccgcagg cgctgctgtc cgaaatcgaa aaatgctggc atcgccaccg taaattgtgg
     3901 ccggaccgca ccatcaatct ggcgctggca atccacgggc aggttgatcc ggtgaccggc
     3961 gtatcgcaaa ccatgccgca agcgccgtgg acaacgccag ttgaggtgaa gtatctgctg
     4021 gaagagaagc tgggcattcg ggtgctggtc gataatgact gcgtgatgct ggcgcaggcg
     4081 gagaaatggc aaaataattc gcaggaacgg gatttctgcg tgatcaacgt tgattacggc
     4141 attggctcgt cgttcgtgat taacgagcaa atttatcgcg gcagtttgta tggtagcgga
     4201 cagattggtc acaccatcgt taatccggat ggcgtcgtct gcgactgtgg acgttacggc
     4261 tgcctggaaa ccgtcgcctc gttaagtgca ttaaaaaaac aggcgcgggt atggctaaaa
     4321 tcacaaccgg ttaatactca gcttgatcct gaaaaactga caaccgcgca gttaatcgct
     4381 gcctggcaga gtggagaacc gtggatcacc agctgggttg atcgctctgc caatgccatt
     4441 ggtttgagtc tgtataactt cctcaacatc ctcaatatta atcagatttg gttgtacggt
     4501 cgcagttgtg cctttggtga gaactggctt aatactatta ttcgccagac aggatttaac
     4561 ccgttcgacc gcgacgaagg accgagcgtg aaaacgacgc aaattggctt cgggcaatta
     4621 agccgcgcac aacaggtgct gggaattggc tatttgtatg ttgaggcgca gttacgacag
     4681 atttgatggc gcgataacgt agaaaggctt cccgaaggaa gccttgatga tcataaacga
     4741 aaaattgcct gatgcgctac gcttatcagg cctacacgga gattgcaata tattgaattt
     4801 acaagatttt gtaggcctga gaaggtgttc atgccgcatc caacatgaac aacgagcaca
     4861 ttgtcagcaa atcaccgttt cgcttatgcg taaaccgggt aacgtgcgca gatgtcgaga
     4921 actttacctt tgatgcgctc gataacggct tcatcattga tgctgtccag cacgtcacac
     4981 atccagccag ccagttcttt cgcttcagct tctttaaagc cgcgacgggt aatcgccgga
     5041 gtacctacac gaataccgga ggtcacaaac gggctcttcg gatcgttcgg tacgctgttt
     5101 ttgttgacgg tgatgttagc acggcccaga gcggcgtctg cttctttacc ggtcaggttt
     5161 ttatcaacca gatcaaccag gaacaggtgg ttatcagtgc cgccggaaac cactttgtag
     5221 ccgcgctcga ggaacacttc taccatcgct ttagcgtttt tcgcgacctg ctgctggtaa
     5281 gttttgaact caggctccat cgcttctttc agagcaaccg ctttaccggc gattacgtgc
     5341 atcaacggac cgccctgacc accagggaaa acggcagagt tcagtttttt gtacagctct
     5401 tcgctaccac ctttcgccag gatcaggccg ccgcgcggac ccgccagggt tttgtgagtg
     5461 gtggtagtaa caacgtgagc atgaggaacc gggttcgggt agacgccagc agcaaccagg
     5521 cccgcaacgt gcgccatatc aacgaacaga taagcaccga tgctgtcagc gatttcacgc
     5581 attttcgccc agtcaaccac gccggaatat gcagagaaac caccgataat cattttcggc
     5641 ttgtgttctt tggcttgttt ttccagatcg gcgtaatcga tatgaccggt agcatcgata
     5701 ccgtaaggaa cgatgttgta cagtttaccg gagaagttaa ccggagaacc gtgagtcagg
     5761 tgaccgccat gcgccaggtt catacccaga acggtatcac ctggttccag cagcgcggtg
     5821 tagaccgcaa agttagcctg ggagccggag tgcggctgga cgttagcgta gtcagcgccg
     5881 aacagttctt tcgcacgatc gatcgccagt tgttcaacga tatcaacata ctcgcaaccg
     5941 ccgtagtagc gtttgcccgg ataaccttca gcatatttgt tggtcagctg agaaccctgc
     6001 gcctgcatta cgcgcgggct ggtgtagttt tcggaggcga tcagttcgat gtgctcttcc
     6061 tgacgtactt tttcctgctc catagcctgc cacagttcgg catcataatc ggcaatgttc
     6121 atttcacgct ttaacatccg catctcctga ctcagctaac aataaaattt ttggccttta
     6181 taggcggtcc tgttggacaa cggcgaacag tataaccgaa tcattgtgcg ataacaggtc
     6241 ttgacaaagg aatttacgca aacgattacc ttaaggctac gcaaggcttt ggagaataaa
     6301 gagcttgcaa ccggaaacgg atttcttttc aggtttgtga tgcaaatttt tcacttcatc
     6361 acattctttc tgaaaaacac caaagaacca tttacattgc agggctattt tttataagat
     6421 gcatttgaga tacatcaatt aagatgcaaa aaaggaagac catatgcttg acgctcaaac
     6481 catcgctaca gtaaaagcca ccatcccttt actggtggaa acggggccaa agttaaccgc
     6541 ccatttctac gaccgtatgt ttactcataa cccagaactc aaagaaattt ttaacatgag
     6601 taaccagcgt aatggcgatc aacgtgaagc cctgtttaac gctattgccg cctacgccag
     6661 taatattgaa aacctgcctg cgctgctgcc agcggtagaa aaaatcgcgc agaagcacac
     6721 cagcttccag atcaaaccgg aacagtacaa catcgtcggt gaacacctgt tggcaacgct
     6781 ggacgaaatg ttcagcccgg ggcaggaagt gctggacgcg tggggtaaag cctatggtgt
     6841 actggctaat gtatttatca atcgcgaggc ggaaatctat aacgaaaacg ccagcaaagc
     6901 cggtggttgg gaaggtactc gcgatttccg cattgtggct aaaacaccgc gcagcgcgct
     6961 tatcaccagc ttcgaactgg agccggtcga cggtggcgca gtggcagaat accgtccggg
     7021 gcaatatctc ggcgtctggc tgaagccgga aggtttcccg catcaggaaa ttcgtcagta
     7081 ctctttgact cgcaaaccgg atggcaaagg ctatcgtatc gcggtgaaac gcgaagaggg
     7141 tgggcaggta tccaactggt tgcacaatca cgccaatgtt ggcgatgtcg tgaaactggt
     7201 cgctccagca ggtgatttct ttatggctgt cgcagatgac acaccagtga cgttaatctc
     7261 taccggtgtt ggtcaaacgc caatgctggc aatgctcgac acgctggcaa aagcaggcca
     7321 cacagcacaa gtgaactggt tccatgcggc agaaaatggc gaagttcacg cctttgctga
     7381 tgaagttaag gaactggggc agtcactgcc gcgctttacc gcgcacacct ggtatcgtca
     7441 gccgagcgaa gccgatcgcg ctaaaggtca gtttgatagc gaaggtctga tggatttgag
     7501 caaactggaa ggtgcgttca gcgatccgac aatgcagttc tatctctgcg gcccggttgg
     7561 cttcatgcag tttaccgcga aacagttagt ggatctgggc gtgaagcagg aaaacattca
     7621 ttacgaatgc tttggcccgc ataaggtgct gtaatttgat gttgccggat ggaaacatcc
     7681 ggcaaccctt gacgcggttt aaattgccgc gtcgtcctct tcaccggtac ggatgcgaat
     7741 gacccgagcc acgtcaaaga cgaagatttt accgtcaccg attttgccgg tttgcgccgt
     7801 gcgaataatg gtatcgacac aggtatcgac aatgtcgtcc ggtacgacaa tctcaatttt
     7861 cactttcggc agaaaatcca ccatatactc cgcgccgcgg tacagctcgg tatggccttt
     7921 ctggcgacca aagcctttca cttcggtcac cgtcatgccg gtaataccga cttcggccag
     7981 tgcttcgcgg acatcgtcca gcttgaaggg ttttataatc gcatcaatct ttttcatgct
     8041 attccttgaa aaggttgcct gtcttttgat ctgctaaacg taacacataa cgccaattca
     8101 ttccttgaaa tcgtttgcat ccagctcgtg tcgggaaagc agtttataaa attctgtccg
     8161 gttgcgcccc gccattctcg ccgcgtgggt gacgttgcct ttggtgattt gcagcagctt
     8221 acgcaaatag ttgagttcaa aatgattacg tgcctcaaca aaggttggca gcgccgtatt
     8281 ttcaccctcc agcgcctgct ccaccagcgc atcactaatc accggagatg aggtcagcgc
     8341 cacgcactgt tcaatcacgt tgaccaactg gcgcacatta cccggccagc tcgcggtcat
     8401 caggcgtttc atcgcatcgg tagagaacgc gcggacaaac ggtttatgtc gctctgccgc
     8461 ctggcgcaac aggtgatttg ccagtagcgg aatgtcttct gtgcgctccg ccagtgccgg
     8521 aattttcagg ctgacaacgt tgaggcggta atagagatct tcgcggaatt ccccgcgcgc
     8581 catcgctttt gacagatcac ggtgagtggc agaaataatc cgcacattga tatcaatatc
     8641 gcggttactg cccagcgggc gcactttacg ctcctgcaac acgcgcagca gtttgacctg
     8701 taacggcgca ggcatatcgc caatttcatc gagaaatagc gttccgcctt ccgccgcctg
     8761 gaataaacct tcgcgattgc tgacagcgcc agtaaacgcg ccacgcgcat gaccaaacag
     8821 ctccgactcc agcaattgct cgggcaatgc gccacagtta atagcaataa atggtttgct
     8881 gttgcgcggg ctggcgttgt ggatagcctg ggcgaaaatc tctttcccgg tgccgctctg
     8941 accgttaatc aaaacgctga cgtctgattg cgccaccagc cgcgcctgtt ccagcaaacg
     9001 cagcatcagc gggctgcggg tgacaattgc ctcgcgccag cgttcatcgg tggctggcgc
     9061 ggattgctcc agcgcatcgt caattgcctg atatagcgca tctttatcga caggcttggt
     9121 gaggaaacta aaaacgccct gctgtgttgc agcaacggca tcggggatag aaccatgcgc
     9181 agtaagaata attactggca ttcccggctg cactttctgg atttcagcaa atagctgcat
     9241 accgtccatt tcatccatcc gcaggtcgct gataactaaa tctacttttt cgcgattcag
     9301 tacccgtaat ccttcagcgc cactttccgc cgtgaccaca ctgtagcctt cgctggtcag
     9361 gcgcaggcca agcagtttca gcaatcccgg atcgtcatcg accaataata aatgcgcagg
     9421 tttatggctc atcaggagtg acctcatggg tggatggcgc gggcttttca ctctcgtgcg
     9481 gcgtatccgg cgagaaattt ccagccggtt tgcgggtcga gagctggcgt tcaatatcgg
     9541 tcaggttttc cagcttgcgg gtggtgagtt ccagttgctg ctgtagaacg tgatgttgct
     9601 ggcgcaatgt atccagctcg ctgtcgctgg actgctggag tttactgtaa cgttggcgct
     9661 cttccgccag ttgcagttgc agcgcctgac cgtcgcgcca gagttgatac agtgggcgaa
     9721 cctgtgccgg gatctcggta cttaacgctt caatacgcgc gaccagctgg cggcgctcat
     9781 acggcgtaat tttggcgtcg gcgagcaaaa tcccttgttt aaaggtattt tgccagctgc
     9841 cgtcgtcata ttggcgagct tgctgacgcg actgcgcagg cattaaacga tcagcacaat
     9901 ccatcgcccg cagccagtaa agcggattgg tttcggttga tttaccttgc agcgcccaga
     9961 tgtcactaca ttcagtagaa agatagtcag ccagttgata aaccggaatt ttttcttctg
    10021 ctggcgtatc aatggctggc ttattgtgat tctgcacgca acccagcaat gccagacatg
    10081 gcagccctgc cagccacagc cgtcggggca ataatcgttg aaaaatgtgt cgcatattca
    10141 ccagacttaa agcctatccc agtgggcgta attgttgcag acagtctgga catggacagc
    10201 gcggagaaac cggggcgtac atatcgtacg tgaagatttc gagcactacc cggggccgaa
    10261 ctgacaaata aaatagcctg ataggatagg ctgttagcat tatttcgtgt ttttcgacga
    10321 cggtaattca atgcggaaac aaacgtcttg cccgctctcg tcgaccagat acagttcccc
    10381 ttgcatacgg cgaatacaat ccctggcaat gcttaatccc agaccgctgc ccttcaccgc
    10441 cccttttcgc tggtggcttc cctgaaaaaa gggttcgaag atcatggcgc gttcctcttg
    10501 cggaatgggc gtgcctgtat tgatgacatc aatataaacc cgcgcaccat gtaaactgct
    10561 gcgaaggcaa atgttaccgg attcagcccc gtagtgcacc gcattggagt aaagattatc
    10621 cagtacgctc atcagcagca ttggctccgc caggcaagct gttgctttga gatcgacgtc
    10681 ggtatgcatc attttagccc gtgcgggcag gctatgagca gaaaccactg tctccaccag
    10741 cggtgctaat tcaacattct ccagttccac cgcactgtcc gcctgtttac ggttgtaatc
    10801 aagcagttgt tcgatcagtt tttgcaaatt gcagctgctg ctatcaagaa tgctcaccac
    10861 ctctttttgc tctggcgtaa gcggcccgac aacctgatca gccagtaatt cagtgccctc
    10921 gcgcatactc gccagtggcg tttttaattc atgagataaa tgtcttaaaa attgatggcg
    10981 ttgggattcc agccatgaca ggcgctcact taaccaaaga atacgttgcc caaccgagcg
    11041 taactcgctc ggtccacaga acgaaacgct attgcccaga gaacgccctt cccccagacg
    11101 gttgatcatg cgctcgatat ttttcaccgg cccgataatc atccgcgtga aaagtagcac
    11161 catcaccaga ctcaccagaa atagcaccag cgattgccaa ccaaaatatt gcccacgttc
    11221 ggcgatttca cgctgaagtt gctgcccacg agagaacacc actgtgcgcg tggcctgcac
    11281 catttcggta ttggcactgg caaaggcttc aagacgcgcg gcggcggcag catcgggacc
    11341 actgttgtta cactgaagtt gggccagatt gttcaaatcc tgacgcaatg cctggtaaag
    11401 tttatcgtct ggcagtacgc ctgcgtgggc atcgagcatt tcgctgtaac gcttgcgctg
    11461 gctttgataa accttcgcaa gcgtagggtc gtccagcacg caatactgac ggtagctacg
    11521 ctccatctcc agcgccgcgt tggtcatcgc ttcactacgc cgggcatcaa taagcgtagt
    11581 gcggttaacc agcgccgcct gatcgctaag cgcattcagg ctttgccagg cttgccatgc
    11641 cagcaccaac aggggtagta gaattagcaa aaatgccagc attaccagtt gtcgtaatga
    11701 gcggggaaaa acgggccagc gtttcaaggt gttactctcg tcagacgcga atagcctgat
    11761 gctaaccgag gggaagttca gatacaacaa agccgggaat tacccggctt tgttatggaa
    11821 taaggcggtg cctaactcga cgtttcgccc gatggttgat atagctacgc tgatatcaga
    11881 agttggacgg caggcacctt gttgtgcgtc attcgaattt tatgtagcac gtcccgaagg
    11941 ggctgacata agtcggtgaa tgagccactg cttactatta tgcagtaact gtgccaataa
    12001 agaaaatagt ttggtaacgt actgattata cgttgctttg agagatttat ggcttcttcg
    12061 ctatttgaat gtaaatcagc cactgtgtcg ctaaaaagag acaacttaag ataaacttat
    12121 tagattacaa taaaatcaat catttaagtg tcgccagaaa gcgacacggt aaaccaccca
    12181 ttgtcgtgat ttacagacac aaaaaagctc ccggagttgg gagcttatga tagtggttgg
    12241 tgcttaatgc cgcatccggg ctgcaaaacc aatgaactga tgacttaccc caactgctta
    12301 cgcgcattgc ggaaaatgcg catccatggg ccatcctcgc cccagttttc cggatgccag
    12361 gagttgctga cagtacggaa aacacgttcc gggtgcggca tcataatggt gactcgacca
    12421 ctttcagtcg taaccgccgt aataccgttc ggtgaaccgt tcgggttagc cgggtaggtt
    12481 tcagtgactt tgccgaagtt atcgacatag cgcagtgcca ccagcccttt gctttccagt
    12541 gccgccagat gcgccgcatc acgcacttcc acgcgccctt caccatgaga gacagcaatc
    12601 ggcatttgcg agcccaccat cccctgcaac agcagagacg ggctttgggt tacttcaacc
    12661 aggctgaaac gcgcttcaaa gcgatcggag gtgttacgca caaaacgtgg ccacaactcg
    12721 ctccccggga tcagttcacg caggttagac atcatctggc aaccgttaca tacccccagg
    12781 gccagcgttt gcggacgatg gaagaaggtt gcaaactcat cgcgtacacg gtcattgaac
    12841 aggatagact tcgcccaacc ttcaccggca cccagcacat caccgtagga gaaacctccg
    12901 cacgcgacca gggcgtgaaa atcctccagt cccgtacgtc cagtcagcag gtcgctcata
    12961 tgcacgtcga tagcatcaaa gcccccacgt tggaaagctg ccgccatttc aacatgcgag
    13021 ttcacgccct gctcacgcag tacggcaact ttcggacgtg cgccagtggc aatatacggt
    13081 gctgccacat cttcgttgat atcgaacgac agttttacat tcaggcccgg atcggcgtcg
    13141 ttagatttcg cctgatgctc ctgatcggca cactccgggt tgtcacgcag gcgctgcatc
    13201 tgccaggtag tttctgccca ccagacacgc aacgtagtgc ggctttcgct gaatacagtc
    13261 tgcccgttgg cggtaatcac aaaacggtca ccggaaaccg cctgcccaac ataatggacg
    13321 caatccgcaa gcccatgctg tgccagtacg gactcgaccg cttcacggtc agcggcacga
    13381 acctgaatca ccgcacccag ttcttcgtta aacaacgccg ccaggcgatc gtcacccaga
    13441 gaggcgatat ccgcgtcaat gccacaatga cctgcaaagg ccatttccgc cagcgttacc
    13501 agcaggccgc catcagagcg gtcgtgatac gccagcagct tacgctgtgc aaccagcgcc
    13561 tgaatcgcgt catagaagcc tttcagttgg acaacatcgc gtacatctgc cggtttgtcg
    13621 ccaagctgac gataaacctg cgccagcgcc gttgcgccca gcgcgttatt gcctttgccc
    13681 aaatcaatca gcagcagtgc gttatcttcg gtacaaagct gcggcgtgat ggtgtgacgt
    13741 acatcttcca cgcgggcaaa tgcagaaatc accagcgaca gcggcgacgt catttcgcgc
    13801 tcttcgttac cttcctgcca gcgggttttc atcgacatag agtctttacc caccgggatc
    13861 gtcaggccca gcgccggaca aagttcttca cccacggctt taacggcttc atacaggccc
    13921 gcatcttcac cagggtggcc tgccgccgcc atccagttgg cggaaagttt aatgcgtttg
    13981 atatcgccaa tctgtgttgc ggcaatattg gttaacgctt caccgaccgc cagacgggca
    14041 gaggcggcga aatccagcag cgcaaccggc gcacgctcgc caatcgccat cgcttcaccg
    14101 tagtagctgt cgaggctggc ggtagtgacc gcgcagttag cgaccggcac ctgccacggc
    14161 cccaccatct gatcgcgcgc caccatgccg gttacgctgc ggtcaccgat ggtcaccagg
    14221 aaagttttct ccgccacggt cggcagatgc agtacgcgtt tcaccgcgtc tgcaatggtg
    14281 atcccttcac ggaccagcgc atcgcccttc gctttcagcg tttgtacatc gcgggtcatc
    14341 ttcggcgttt taccaagcag gacgtccagc ggcagatcga tcggctgatt atcaaaatga
    14401 cgatcgtgca gagaaagatg cagttcttcg gtcgcttcac caatcaccgc gtagggtgcg
    14461 cgctcacgct tacacagttc gtcaaacagc ggtaattgat cggcagcaac cgccagcacg
    14521 tagcgttcct gggattcgtt acaccagatt tccagcgggc tcatgcccgg ttcgtcgctt
    14581 agaatatcgc gcagttcaaa tttaccgccg cgtccgccgt cgctcaccag ctccggcatc
    14641 gcgttagaaa gaccgcccgc accgacgtcg tggataaaca gaattgggtt agcatcacca
    14701 agctgccagc aacggtcgat cacttcctgg caacgacgct ccatctccgg gttgtcgcgc
    14761 tgtacggaag caaagtcgag gtcggcatca gactgaccag acgccatgga agacgccgcg
    14821 ccgccgccaa gaccgatgtt cattgccggg ccgccgagaa cgaccagctt cgcaccgacg
    14881 ttgatctcgc ctttttgtac gtgatcggcg cgaatgttgc cgatcccgcc cgccagcatg
    14941 atcggtttgt gataaccgcg cagctcttcg ccattgtggc tgttcacttt ttcttcataa
    15001 gtacggaagt agccgttcag tgccggacga ccaaattcgt tgttaaacgc cgcaccgccc
    15061 agcgggcctt cggtcatgat gtccagcgcg gtaacaatgc gctcaggctt accgaaatct
    15121 tcttcccacg gctgttcgaa gccaggaatt cgcaggttgg atacggagaa accaaccaaa
    15181 ccagctttcg gcttcgcgcc gcgcccggtg gcaccttcat cgcggatttc accgccggaa
    15241 ccggtcgccg cgcccggcca cggagaaatc gccgtcgggt ggttgtgagt ttcgactttc
    15301 atcaggatgt gcgccggttc ctgatggaaa ttgtagcggc ccgtttcgtg gtcagcaaag
    15361 tagcggccca cttcagaacc ttccattacg gcggcgttat ctttataagc agagagaacg
    15421 tgatctggcg tggtttcgaa agtatttttg atcatcctga acagcgattt cggctgttgt
    15481 tcaccgtcga taatccagtc ggcgttaaaa attttgtggc ggcagtgctc agagttcgcc
    15541 tgagcgaaca tatacagctc gatgtcgttc gggttacgac caagctttgt gaaagcatcc
    15601 tgcagatagt caatttcatc ttccgccaga gccaggccaa gacgcaggtt agcgtcgatc
    15661 agcgcctgac ggccctgccc cagcaaatca acgcttgtga ccggagtcgg ttgatggtgg
    15721 gcaaacaact gctctgcatc atctaaatca aaaaagaccg tttccatcat gcggtcgtgc
    15781 agttcagcgg taacctgctg ccattgttca ttggtcagcg taccggcttc tatatagtaa
    15841 gcaacgccgc gctcaaggcg gtttacctgt tgtagcccgc agttatgggc aatatcggtc
    15901 gctttcgaag accagggaga gatggtgcca ggacgcgggg tcaccagcag gagtttgcct
    15961 tgcggggcgt ggctggcgag tgccggacca tatttcagca ggcgttcaag ttgtgcgtgc
    16021 tcatcatcgt ttaacggcgc attgaggtca gcaaaatgga catactcggc gtaaatattg
    16081 tgaaccggga gcctggcagc ctgaaaacgt gccagcagtt tgttgattcg gaatgccgac
    16141 agtgcaggcg aaccacgcag aatttccatc ataagtctct cgtcttctaa gctttcggtg
    16201 tacccaaggg ggggaaacgg gcgtcattat aaagaatctg atgcgctgac gaaaccgttt
    16261 gcgtggaaat aaaatcacca tcgtgaatta gcaacgcgtg ccgccaatgg ctgtaataag
    16321 ttgccatctg gcgcaggttt acgcaaaatg ccgctcattt atgagtaaac ctttcactat
    16381 tattacgttt tttcaagctg ggacgcgcac gacacagaga attaactaat tgaaaaaatt
    16441 aaagattaat tatctgttca tcggcattct ggcactgctg ctcgcggtcg ctctctggcc
    16501 atccattccc tggtttggta aagccgacaa ccgtatcgcc gccattcaag cgcggggaga
    16561 gttgcgtgtg agcaccattc atactcccct gacttataac gaaatcaacg ggaaaccttt
    16621 tggcctggat tacgaactgg cgaaacagtt tgccgattac ctcggcgtaa aactgaaagt
    16681 gaccgtgcgg cagaatatca gccagctgtt tgacgacctt gataatggta acgccgacct
    16741 gctggcggca ggactggtct ataacagtga gcgggtaaaa aattatcagc ctggccctac
    16801 ctattattcc gtgtcacaac aactggttta taaagtgggt cagtatcgcc cacgtacgct
    16861 gggcaacctg acggcggagc aactcaccgt tgcaccgggt catgtggtgg ttaacgatct
    16921 ccagaccctg aaagaaacaa aattcccgga attaagctgg aaggtagacg acaaaaaagg
    16981 ctctgcggaa ttaatggaag atgtcatcga aggaaaactc gattacacca ttgctgattc
    17041 tgtcgccatc agcctgtttc agcgcgttca cccggagctc gccgtagcgc tcgatatcac
    17101 cgatgaacaa ccggtgactt ggtttagccc gttagatggc gataataccc tttccgccgc
    17161 cctgctcgac ttcttcaacg aaatgaatga agacggtacg ctggcacgca ttgaagagaa
    17221 atacctgggg catggcgatg attttgatta cgtcgatacg cgcacatttt tacgcgccgt
    17281 cgatgcggta ctgccgcagt taaagcccct gtttgagaaa tacgccgaag aaattgactg
    17341 gcgtttgctg gccgctattg cttatcagga atcgcactgg gatgcacagg ccacttcacc
    17401 gacgggtgtg cgcggcatga tgatgttaac caaaaatacc gcgcaaagcc tcggcattac
    17461 ggatcgtacc gatgccgaac agagcatcag cggtggcgtg cgttatttgc aggatatgat
    17521 gagtaaagtg ccggaaagtg tgccggagaa cgaacggatc tggtttgccc tcgctgcgta
    17581 caatatgggc tatgcgcata tgctggatgc ccgcgccctg acggcaaaaa ccaaagggaa
    17641 tcctgacagt tgggctgacg taaaacagcg tctgccttta cttagccaga aaccctatta
    17701 cagcaagctg acttacggct acgctcgtgg gcatgaagcc tacgcttatg tcgaaaatat
    17761 tcgtaagtat cagattagcc tggtgggtta tctgcaagag aaagagaagc aggctacaga
    17821 agcggcgatg caactggcgc aggattatcc ggcggtatcg cctacggagt tgggcaaaga
    17881 gaaatttcct tttctctcgt ttctttccca gtcgtcatca aactatttga cccattctcc
    17941 ctctctgctg ttttccagga aagggagtga agagaaacaa aattaatccg tcgaggattg
    18001 cgcttttttc tgcgctttaa tttcctggcg gcgcatgcga aagaagtcac tgagcaacgc
    18061 cgcgcactca tccgccagta ttccttccgt aatttccact cggtgattca tacccggatg
    18121 atgcagcaca tccattaaag atcccgcagc gccagttttc gcgtcacgcg caccaaagac
    18181 cacgcgacca atgcgactgt ggatcatcgc tccggcacac attacacatg gttcaagcgt
    18241 gacatacaac gtggcgtcga tcagacgata attttgcatc accagaccac cctgccgcag
    18301 ggccatgatt tctgcatgtg cggtgggatc atggcgacca atcgggcggt tccagccttc
    18361 gccgattacc cgattgttat gcactaatac cgcgccgacc ggcacttccc gctcatccca
    18421 ggcacgtttc gccagcgtca gcgcgtgacg catccagtat tcgtggctaa attcgacttc
    18481 agacaaaaag aaaactccgg ttataaaagc gcggcgcatt atacacggac gctatgcttt
    18541 actctattcc agttgctgga gttcaccgcg cggggttact cgccaacgat gctgacagaa
    18601 ataaagcagc ggattgtcct gattactgtc gctatagcca ctgtacagcc gcagcggcgt
    18661 gccgattttg cgctccagtt gtgcgacctt ttcatgtccc agacaacgca tcgtcaatac
    18721 ccaaccacca tagccacgct gaatttggct ggcgataaga ttaacccgcg gcagccaggg
    18781 cgtatcgaaa taaaccgctt caaccagcgg ctgcggagag ccggtaatca accagatatc
    18841 agcatcggaa cttaacaggt aggtggttaa tcgctcctga accagcggaa aggcggtaac
    18901 attgtcgcga aaccagcgca cgaaatcggc ctgcaacgtc tgtaaacgtg cttcgctgtg
    18961 accaaaagtg cacccccaca gaagcagact catcggccag cgtgccgcac gaccttttat
    19021 caataacgca atggctataa tcggtaacaa cggcaggaca agtaacgcat tcagcggttg
    19081 gcgacgcagt aaatagcgca gaaaactgcc gaacatatcc tgctgatgca atgttccatc
    19141 taagtcaaaa aacaccacac gacgctcgtg agttgccaaa ccatactcct ctggactgaa
    19201 acatcctgat taattttcgc tactacatag cctaacagat agatcatcac ttttccggca
    19261 acaatcgacc gcatcatggc taactggaat ttttaattca tggcaattag cggcaatgga
    19321 atataaaatt cactcgcgtg tgtctcatat tgcctgttgt cgccactatt tcatcgcgag
    19381 gaaagatatg aacggcttac ttcgtatccg tcagcgttac caggggcttg cccagagtga
    19441 taaaaaactg gcggattatc tgctgctaca acctgatacg gcgcgccatt taagctctca
    19501 gcaactggcc aacgaagccg gagtcagtca gtccagcgtc gtgaagttcg cgcaaaaact
    19561 cggctataaa ggttttccgg cgcttaagtt ggcgttgagt gaagcgctgg caagccagcc
    19621 ggaatcgccc tccgtgccca ttcataacca aatccgcggt gatgatccgt tacggctggt
    19681 cggcgaaaaa ctgattaaag aaaatactgc cgctatgtac gcaacgctaa acgttaatag
    19741 tgaagagaaa ctgcatgaat gcgtaacaat gttgcgctct gcgcggcgga taattctgac
    19801 cggtattggc gcttcgggtc tggtggtgca aaactttgcc tggaagctga tgaagattgg
    19861 cttcaatgct gccgcactgc gcgatatgca tgcgctactc gcaacagtac aggcgtcgtc
    19921 ccctgacgat ctgttattag ccatttccta caccggtgta cgacgcgagt taaacctggc
    19981 ggcagatgag atgctgcgag tgggcggaaa agtgctggcg attaccggct ttactccgaa
    20041 tgccctgcaa cagcgtgctt ctcattgcct gtataccatt gccgaagaac aagcgacaaa
    20101 cagtgcttca atctctgctt gtcacgctca gggaatgtta acggatttgc tgttcattgc
    20161 gctgattcag caggatctgg aactggcacc agaacgtatt cgtcatagtg aagcgctggt
    20221 gaaaaaactg gtctgagtaa agaatgcgcg tataatgccc gcccggtttg tgttgttttg
    20281 agagtttcct tatggcgctg ttaatcacta aaaaatgcat caattgtgat atgtgtgaaa
    20341 tcaaataaaa acacatttat tttcatagtg atacagcaat gtgttattga atgtgttgtt
    20401 ggatttatgg tgcaaaaaaa ttttgcataa gttcagcaac acaaagaaca ccactttctt
    20461 taacgtcctc cgcgccctaa aggacggggc tttacggcgc aaaaggcctg tgacatacaa
    20521 cattaacttt tcagtcaacc gtagcacgtc cttgtatacg ccgtgtctgc ggttttatat
    20581 aaccctggct ggaaacctct tatacaaagt cgattcgcca acatcataga tgatcgccac
    20641 cttctggcga gaaactcctg atgcaattag tcgcccggcc tgcgcccatt attctggtga
    20701 aagtttggga cggcgaccgc caattcgtcc ctgtgcgcga gcagcttcca gtcctgcttt
    20761 tgttcgttca acaatcagtt cacgttccat ttcagccagg gcacccatca catggaagaa
    20821 aaagcgtccc attggtgtgc tggtatcaat tgaatcagtc agactacgaa agttgatgcc
    20881 tcgttcgcgc aactcctcca ccagcacgac aagatgccgc atactgcgcc ccagccgatc
    20941 cagcttccag acaaccagtg tgtcgcctgc cgataatgtc ctgagcagtt ttttcagccc
    21001 cggtctttcg gactttgtac cacttatctt gtcttcaaaa atcagctcgc atcctgcaca
    21061 gttcaacgca ttacgttgta gatcggtatt c
//
LOCUS       DAETPZ010000066        20686 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.066, whole genome shotgun sequence.
ACCESSION   DAETPZ010000066 DAETPZ010000000
VERSION     DAETPZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.066"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="K0W59_002764"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="K0W59_002764"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HBI3023737.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="K0W59_002765"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="K0W59_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HBI3023738.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="K0W59_002766"
     CDS             complement(2649..3200)
                     /locus_tag="K0W59_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HBI3023739.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="K0W59_002767"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="K0W59_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HBI3023740.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5215
                     /gene="cybC"
                     /locus_tag="K0W59_002768"
     CDS             4829..5215
                     /gene="cybC"
                     /locus_tag="K0W59_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HBI3023741.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="K0W59_002769"
     CDS             complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="K0W59_002769"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HBI3023742.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="K0W59_002770"
     CDS             complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="K0W59_002770"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBI3023743.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8414..10069)
                     /gene="treC"
                     /locus_tag="K0W59_002771"
     CDS             complement(8414..10069)
                     /gene="treC"
                     /locus_tag="K0W59_002771"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBI3023744.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHTESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLTKPDFVALKTLFRHWQQGMHNVTWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKFIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10119..11540)
                     /gene="treB"
                     /locus_tag="K0W59_002772"
                     /pseudo
     CDS             complement(10119..11540)
                     /gene="treB"
                     /locus_tag="K0W59_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
     gene            complement(11659..12606)
                     /gene="treR"
                     /locus_tag="K0W59_002773"
     CDS             complement(11659..12606)
                     /gene="treR"
                     /locus_tag="K0W59_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HBI3023745.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12791..12844
                     /gene="mgtL"
                     /locus_tag="K0W59_002774"
     CDS             12791..12844
                     /gene="mgtL"
                     /locus_tag="K0W59_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HBI3023746.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12985..15681
                     /gene="mgtA"
                     /locus_tag="K0W59_002775"
     CDS             12985..15681
                     /gene="mgtA"
                     /locus_tag="K0W59_002775"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBI3023747.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="K0W59_002776"
     CDS             complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="K0W59_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HBI3023748.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="K0W59_002777"
     CDS             complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="K0W59_002777"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HBI3023749.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="K0W59_002778"
     CDS             complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="K0W59_002778"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBI3023750.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKNKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSQTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="K0W59_002779"
     CDS             complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="K0W59_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HBI3023751.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18174..18569)
                     /locus_tag="K0W59_002780"
     CDS             complement(18174..18569)
                     /locus_tag="K0W59_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBI3023752.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTEVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="K0W59_002781"
     CDS             complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="K0W59_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI3023753.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19485..20078
                     /locus_tag="K0W59_002782"
     CDS             19485..20078
                     /locus_tag="K0W59_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBI3023754.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20223..20675
                     /gene="tabA"
                     /locus_tag="K0W59_002783"
     CDS             20223..20675
                     /gene="tabA"
                     /locus_tag="K0W59_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HBI3023755.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
BASE COUNT         4916 a         5208 c         5489 g         5073 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc ggatggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgcta gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggctcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcactgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc
     5161 tgctgcagag caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct
     5221 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
     5281 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
     5341 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
     5401 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac
     5461 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
     5521 cccctggcgt ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt
     5581 ggtaaatggc tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
     5641 atgaacacac ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac
     5701 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc
     5761 atccggtaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
     5821 aaatgtaggc cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag
     5881 attaacctat ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac
     5941 cagcgttaaa cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg
     6001 acacacggga ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact
     6061 cgaactcacc ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat
     6121 acggtacatg ctgatagctg taatcccaaa catcttccag cgctttcagg ttgtgctgaa
     6181 tgtttggata ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt
     6241 caaagtcgat cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg
     6301 tgtagtaacc tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc
     6361 agaagcggtc gcacaggttt tcactcggcg tgctgtagag actgaaacca taacccgttt
     6421 cttctttcca ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc
     6481 gaagcttctc gttgtcgtaa acatgctcgc cgccgaacag cgcgttaatg gtttcgtgga
     6541 tgccgatgta acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat
     6601 cgtcagcatt cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg
     6661 ctttcacgcc ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac
     6721 gttcatccag cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg
     6781 gcaggttcag gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt
     6841 cattttccca cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga
     6901 ctttcactac ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca
     6961 gcgccagctg tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa
     7021 tcgcaaacac cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac
     7081 ggttacgcag gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac
     7141 caaaggttac aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca
     7201 gcgactggaa ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt
     7261 ccgggatgtt ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg
     7321 gcgccagcac ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct
     7381 gagcaataat ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct
     7441 cggcgttccc cattttaaag ccctgggtca gcatgccttt caggtcgatc aacatgcagt
     7501 tgaacatcgg gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat
     7561 gtgcctgcac cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc
     7621 cggccagcag gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga
     7681 gtaacgaggc gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt
     7741 tttcacgttc aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt
     7801 atggacccga catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt
     7861 tgcgcccctg catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat
     7921 cgacttccgc tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg
     7981 gcactttgca gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag
     8041 aacaggttat ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg
     8101 tgctttccgc aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt
     8161 agtagcaatt atagtcgatt aatacaacat attgggttgg gacgcatttt aaagtctata
     8221 tatagtgctt tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca
     8281 gagcgtacgg atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg
     8341 aaaaatcaac aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg
     8401 gcttagggaa gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg
     8461 cacagggttg tggtgaggct tcttcgtagt tatgcatcac aagctgccag ttgccgcgca
     8521 tttgccctgg ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt
     8581 gccccttcca ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt
     8641 aattgcccca tgtcaggatg gcttcttgct tacgcagtgc gatgaacttt tggtaggtgt
     8701 aaaacacaga ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc
     8761 ctaggccaat ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca
     8821 tgggcgtgcg gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat
     8881 cacgaccatc gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag
     8941 taatgcgcgt gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg
     9001 ttccctgcat gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac
     9061 cttcatcacc aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat
     9121 tccatgtcac gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa
     9181 agtcaggttt agtcagcgtc catttttcac caccgggata atcgaccttc aggtgatgaa
     9241 aattaaaggt catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa
     9301 ggctggtgga ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt
     9361 tcatctcgtg caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc
     9421 catccaggtc ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca
     9481 acccgtcgac cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg
     9541 ctggattctc ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt
     9601 cgctttccgt atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg
     9661 gcgtttctgg ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca
     9721 gcgcctcgcg aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga
     9781 tacgaatccc gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt
     9841 aggtgggatc aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga
     9901 cataaaaggg ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt
     9961 ggataacgcc acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat
    10021 aaatctggta gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc
    10081 tgcaaatgcg agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc
    10141 aggcggtatt tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata
    10201 gccattgcca gcgcaaatac ctgccagtag ctcggttgaa tcgagagaat tcccggcagg
    10261 ccccctacgc cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga
    10321 ccagaaccaa tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt
    10381 gcaggctcag tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca
    10441 ttgtgcttgc ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac
    10501 agcgcaatca gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata
    10561 gcaagggtgg tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat
    10621 gccgcaccaa tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca
    10681 tcgccaatca tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc
    10741 agcgaacaga cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga
    10801 gtttcaataa cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag
    10861 cccactttgg cgatgctgaa catgccaaag tcccacactt caggcagctg ctgcccgagc
    10921 agataagcgt tcatcagctg ggtggaaacc agtgtcacgc caagcacgat accaaggatc
    10981 ggcgtgccgc ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa
    11041 atcgcttcac cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc
    11101 gccagcgtct gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc
    11161 aaaccgccgc taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc
    11221 aactgctcat gctatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc
    11281 tgcgcctgtc cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc
    11341 accacctgaa attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt
    11401 tctttcggtc tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg
    11461 ctcaccgtcg caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt
    11521 tggtttattt tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa
    11581 tatattgcgc ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat
    11641 ttatcgttca ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct
    11701 gcgcccggtt acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc
    11761 aggatctacg gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct
    11821 cgccagttgc aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag
    11881 cgtgtcggtt gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc
    11941 atagccttgc ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt
    12001 gcagaacgcc aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac
    12061 gccgagataa ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat
    12121 tgccccttcg tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac
    12181 cagcgatgac tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac
    12241 tacgccgtcg atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga
    12301 aaactgattt tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt
    12361 ttgaacggcg agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt
    12421 atcgctttgc ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat
    12481 cactgcttca acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg
    12541 ggaaaccgta gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt
    12601 ttgcatcctg ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca
    12661 ttcagcaatg ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc
    12721 cataaatgct acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac
    12781 cggaggttat atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg
    12841 gtaagcccgt cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct
    12901 gctcagcttt attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt
    12961 tcagacagtg cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat
    13021 ttaccttccc gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg
    13081 gcggtcccgc cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa
    13141 ttgtggaaaa cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc
    13201 cgcgaacaac atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat
    13261 ttatgggtct gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct
    13321 tacgccacgg aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg
    13381 ttgctgaact ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg
    13441 gtcagtaata ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg
    13501 gagatcccga tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg
    13561 atcccggcag atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta
    13621 accggtgagt ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat
    13681 ccgctggagt gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa
    13741 gcaatggtga ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt
    13801 gagcaggaaa gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg
    13861 attcgcttta tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc
    13921 gactggtggg aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg
    13981 ttgccgatga ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa
    14041 gtgatcgtca aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact
    14101 gataaaaccg gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc
    14161 ggtaaaacca gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga
    14221 cttaaaaatc tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg
    14281 ctggccagcc gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg
    14341 tcggtggtag tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag
    14401 gaaatcctca atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac
    14461 accatgctgc gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg
    14521 gttgcggtgg cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa
    14581 tccgacctga tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct
    14641 ccggcactga aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt
    14701 gagttagttg cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt
    14761 ggtagtgata ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg
    14821 ctgtttgccc gcctgacgcc gatgcataaa gaacgcattg tgaccttact gaagcgcgaa
    14881 gggcatgtgg ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg
    14941 gatatcggca tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc
    15001 ctgctggaaa aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc
    15061 gccaacatgc tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc
    15121 gtgctggtgg cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt
    15181 cagaacctgc tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag
    15241 caaattcaaa agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc
    15301 ggaccgatca gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat
    15361 gccaacacgc cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg
    15421 tcgcaaacgc tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt
    15481 gcatcgtggc cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg
    15541 ttttcaccgc tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg
    15601 ctggttgcga ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc
    15661 cgtcgttacg gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag
    15721 acaaagtgag ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc
    15781 aataaattgc aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt
    15841 gtcatcagac ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag
    15901 cgattgcttc gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acacaagaac
    15961 gtgccgggaa agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg
    16021 cgaagtcgtt cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc
    16081 cagcggcttc gacgattgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt
    16141 ctgccggtac ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat
    16201 tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga
    16261 tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc attataggga
    16321 gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt
    16381 tttcacagta tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg
    16441 atgaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga
    16501 tgcgctccgg cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa
    16561 ccgtggcttg cggtgcatac aatgccagtt ggtctacttg atcttcactc aaaaaggtat
    16621 tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg
    16681 tgatgcgctg atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg
    16741 cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt
    16801 gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg
    16861 cctggcgagc gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat
    16921 ccacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt
    16981 tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg
    17041 acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca
    17101 cttcttcaat agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa
    17161 tgtattgcgg cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact
    17221 tcgctagcgc ctgggtctgg gagtgaacgg tgcggccata tttcaggtca ccaaccattg
    17281 cgacgtggag attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg
    17341 tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa
    17401 attcggtggc caggcgcgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt
    17461 aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat
    17521 tggcgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag
    17581 agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg ttcttcaaca
    17641 gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat
    17701 cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt
    17761 atctcctgac gcctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca
    17821 acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa
    17881 cacactgaac catacatcct cccggaaaat tgtccggcat tatactcata gccagaagcg
    17941 gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa
    18001 aatatatgca tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat
    18061 tgcagacatt tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc
    18121 cattcatccg acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc
    18181 tcagggatac gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc
    18241 acctccgtcc agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg
    18301 tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa
    18361 gtgcatcccg cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc
    18421 tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc
    18481 aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca
    18541 gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca
    18601 gaagatttat atttaccggg agccgctttt agggggacga cgttagtaaa caaaacccag
    18661 acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat
    18721 caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca
    18781 taccagcgac ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat
    18841 cgcgcattgg cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgacgt
    18901 tgatggtgat ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg
    18961 cagatttact ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg
    19021 agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat
    19081 gataaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca
    19141 gggcatcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct
    19201 tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc
    19261 cagtctcttg tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat
    19321 tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga
    19381 ggatgagaac tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac
    19441 atgcattaca aaactgtttt aactttctgt caacaggttt tgtaatggtc actaaaaaac
    19501 aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa
    19561 aagtgctttt tcaccaaaaa ggtttcgatg cagtaagtgt ctctgaagtt actgattatc
    19621 ttggtattaa ccccccgagc ctctacgcgg cttttggcag taaggctggg ttatttagcc
    19681 gtgtactcaa tgaatatgtc ggtacggaag ctattccgct tgccgatatt cttcgtgatg
    19741 atcgtccggt aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc agatatagcc
    19801 aaaacggcgg ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat gatccacaag
    19861 cgcgtgatat tgccattcaa tattatcacg ccgcagaaac gaccatttat gactatatcg
    19921 ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag
    19981 ggctttctgc gaaggcacgg gaggggcact ccatcgaaca actctgtgca acagctgccc
    20041 tggcggggga agcgataaaa actcttctca aggagtgatg ctggccttga tccgaaaggc
    20101 gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggcgga
    20161 attgatcatc ttttcgtata acgatagaaa tgaagcattg ttttaattaa ggagtggaaa
    20221 agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg
    20281 cgattgagca tatcaaagca cacgttacgg cagaaacgcc aaagggcaag cacgatatcg
    20341 aaggcaatcg tctgttttat cttatctcgg aagatatgac cgagccgtac gaagctcgcc
    20401 gtgcggagta ccatgcccgc tatcttgaca ttcagattgt gttaaaaggt caggaaggca
    20461 tgaccttcag cacgcaacct gcaggcgcgc cggataccga ctggttagct gataaagaca
    20521 tcgcattttt gccggaaggc gttgatgaga aaacagttat cctaaatgaa ggtgattttg
    20581 ttgtgtttta tccgggggaa gtgcataaac cgctgtgcgc agtgggcgcg ccagcccagg
    20641 ttcgcaaagc agtagtgaag atgctgatgg cgtgatgact tttcgc
//
LOCUS       DAETPZ010000067        20672 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.067, whole genome shotgun sequence.
ACCESSION   DAETPZ010000067 DAETPZ010000000
VERSION     DAETPZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.067"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="K0W59_002784"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="K0W59_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HBI3023756.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMLRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQSEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="K0W59_002785"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="K0W59_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HBI3023757.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="K0W59_002786"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="K0W59_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HBI3023758.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIHEQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="K0W59_002787"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="K0W59_002787"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HBI3023759.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGRVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="K0W59_002788"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="K0W59_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HBI3023760.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGTRVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="K0W59_002789"
     CDS             complement(8217..9110)
                     /locus_tag="K0W59_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HBI3023761.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="K0W59_002790"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="K0W59_002790"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HBI3023762.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLVEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="K0W59_002791"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="K0W59_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HBI3023763.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="K0W59_002792"
     CDS             complement(10241..11473)
                     /locus_tag="K0W59_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HBI3023764.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="K0W59_002793"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="K0W59_002793"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HBI3023765.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVVMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="K0W59_002794"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="K0W59_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HBI3023766.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16497)
                     /locus_tag="K0W59_002795"
                     /pseudo
     CDS             complement(14278..16497)
                     /locus_tag="K0W59_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16704..16988)
                     /gene="ihfB"
                     /locus_tag="K0W59_002796"
     CDS             complement(16704..16988)
                     /gene="ihfB"
                     /locus_tag="K0W59_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HBI3023767.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17148..18821)
                     /gene="rpsA"
                     /locus_tag="K0W59_002797"
     CDS             complement(17148..18821)
                     /gene="rpsA"
                     /locus_tag="K0W59_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBI3023768.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18932..19615)
                     /gene="cmk"
                     /locus_tag="K0W59_002798"
     CDS             complement(18932..19615)
                     /gene="cmk"
                     /locus_tag="K0W59_002798"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBI3023769.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19788..20552)
                     /gene="ycaL"
                     /locus_tag="K0W59_002799"
     CDS             complement(19788..20552)
                     /gene="ycaL"
                     /locus_tag="K0W59_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HBI3023770.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4968 a         5713 c         5019 g         4972 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttcctg gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg actgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcaaacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc agcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgt ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt gcttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactctg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgctg gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtacca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc atattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaaacct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc caccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcctcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcaccac tacatgttca agctgagcaa
    11821 cgtcacaccg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccagaccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatacgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcaatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgac cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggctatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagg ccagtcctgt gtcatagaga atcgctttgc cgtttctggc
    15001 tatcaccatt gccagccctt gcccgacatc aagcatgtac agctgccact cgtcaggtcg
    15061 aggtttttgc cacagcggcc agcacatcag caagcctcca gccacacaca ttactggcaa
    15121 cgttcgccag gcgtttaatc accataccac cagtaagaac catggggaaa atgatagcca
    15181 ttgccaacat tcaacaatgt tgatccaccc ttccggcaaa ctctttaacc cctgttccag
    15241 gattaacggc ccgctaaaat gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa
    15301 tgtcaccaag ggaattgcaa atagatttgc aataaacgag gtcagactaa tgccatgaaa
    15361 tatgacgatt tgcatgggca taagcaggag tgtgattccc agttgcagat ggatgaggga
    15421 aacaactgca cgcaataccg gcggcagttg ccactcagga cagggaaacc actgatacca
    15481 aaaaatcagt gccgcgaccg cagcggcaga gagccataaa ctttgcgaga gaatggcaac
    15541 aggatccatc agcaaaattg ccgccagaca acatatccat acatcccagc cactccactg
    15601 tcgcccactt aacttaagca ttccccacgt agcaagcgcc accacggtac gcaatgcagg
    15661 aggttgcatt cccgtcaacc aggcataaaa agcagcacag cagattccgc caattaatgg
    15721 tatttgccag tggatccagc gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc
    15781 agccagcaac gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc
    15841 agtatcgcgc atgatatttt tgatttcttt ggggacggat aaccgttccc ccatacctaa
    15901 accaagaata accgcattcc acggataggg ttgcagcgtt gtttgtagtg acgccagata
    15961 ctgtgcacgc aggctacaat tcggttcaat gacacttgcc tgcagaaaac ggccggtgag
    16021 cggctgatgc tgggcaatgg cataacgctg gctatcaaag ccgccatcat taagttggcc
    16081 atgaactgca cgaactttga gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg
    16141 aagttattcg ccatacagca caaggcctgg cgcagggaat atacgtcgac cttgtagata
    16201 agtaatttga ccgtaatgag tggtcatgcc gtcagttgca gtgatctcaa caattgcatc
    16261 ctgcgtcgcg ccagttaagg tttctcctgc ccacaaaatt tgctttgctg ataatatgcc
    16321 ccaaacgaaa aacagcaacg tcagcgcgat ataacggacg gttttaacag ggataaatgc
    16381 cagtacgcag gcgaagagag tcagaaacgc aagggttaat gtcctaggca attggggcaa
    16441 aatcagcaac ggaaaaattc cgcaaattat gcatacgccg actgtcgtta ttttcatgca
    16501 cccctccctg tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt gggaattttg
    16561 cgttccccga ggcacattca gaagaattta acgaaagggt gaaaactgct caaaactctg
    16621 cgaggcagct tccagttcct gattagagaa ttgaaaaaag cacccgacag gtgcttttct
    16681 cgcgttcaag tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg cagttcttta
    16741 ccaggtttaa agtgaggaac gtattttcct tccagttcta ctttatcgcc agtcttcgga
    16801 ttacgtccgg tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc gcggatttca
    16861 atacgctcgc cctgcgcaag agtcgaggcc atatgctcca gcatctcttt tactgcatct
    16921 tcaaccgtct tggcgggaat gtgcgattgc tgggtggcaa gtctttctat caattctgac
    16981 ttggtcatga ttcctccggt tccttaaagg caaattaagc ggctgctgtt gctgcattga
    17041 ttaagggcgg ccgtagccgc ccttagtgct tgattacagg acgaaacctg caatctgtca
    17101 agtaaactca acaaacttcg gaataaaaat cccgaagagt cagagaatta ctcgccttta
    17161 gctgctttga aagcttcagc cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta
    17221 acagttgcga ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga caggctgatt
    17281 gcgcggtttt tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga
    17341 accagggtag cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag gtaaccttca
    17401 acgccgtcag ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt
    17461 acgatagcgc ctttcttgtt cagagcaacc cagttgttga acggatcttc tgcgagctgt
    17521 ttaacgccca gggagatacg ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt
    17581 tcgtcgcctt ttttgtattc acgaactgct tcttcgcctg caacgttcca ggagatgtca
    17641 gacaggtgaa ccaggccgtc gatgccgccg tccaggccga tgaagatacc gaagtcagtg
    17701 atagacttga ttttaccttc aacacggtcg cccttgttgt gggtttccgc gaactgctgc
    17761 cacgggttag ctttgcactg tttcagccct agggagatac gacgacgttc ttcgtcgata
    17821 tccagaacca taacttccac tacatcgcca acgttaacaa ctttggacgg gtggatgttt
    17881 ttgttggtcc aatccatttc ggaaacgtgt accagacctt caacgccttc ttcgatttca
    17941 acgaagcagc cgtagtcagt caggttggtc acgcgaccag tcagtttggt accttccgga
    18001 taacgtttag cgatagctac ccacggatct tcgcccagct gtttcaggcc cagggataca
    18061 cgggtacgtt cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc cacgttgacg
    18121 atttcgctcg gatgcttaac gcgtttccag gccatgtcag tgatgtgcag caggccgtca
    18181 acgccgccca gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat acctttaact
    18241 tccatgcctt cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg
    18301 ataacggcac gacgagaaac aacaacgttg ttgcgcttct gatccagctt gattacttta
    18361 aattcaagct ctttgccttc caggtgcaga gtgtcacgca ccggacgaac gtctaccaga
    18421 gaacctggca ggaacgcacg aataccgttc agctcaacag tgaagccgcc cttaactttg
    18481 ccgttgataa caccggtaac agtttcagca tcttcgtaag ctttttccag cgtgatccag
    18541 gcttcgtgac gtttagcttt ctcacgggac agcagagttt caccgaagcc gtcttctact
    18601 gcgtccagag caacgtcaac ttcgtcacct acctggattt ccagctcgcc ctgggcgttt
    18661 ttgaactgct cagccgggat ggcggactca gatttcagac cagcgtcaac cagtactacg
    18721 tctttgtcga tagcaacaac aacgccacga acgatagaac ccgggcgggt ttcgatttct
    18781 tttaaggact cttcaaagag ttgagcaaaa gattcagtca tgtttaatct tcaggtttat
    18841 atttaacgtc cacctggctc catgccggat ggggttgttt aacatacccg ctgtcattcc
    18901 attgcaacgg gggtactgca aattcggtcg cttatgcgag agccaatttc tggcgcgcgt
    18961 attgtagcgc tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc aacactaaag
    19021 catcggctgc cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt
    19081 tgatctcggc caaaaggcgc tcgaagttaa cactaaagcc cttctcctgc aactgtagca
    19141 tgcggcgatg cgcacgttct tccgaggagg cgtcaaggaa aattttcact ggtgcatcag
    19201 ggaataccac cgttcccatg tcgcggccat cggcaatcag acctggtaat tcgcgaaacg
    19261 cgcgttggcg acgcaataat gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg
    19321 cattcgccac ttcctgagta cgaatttcgc cgctgacatc ttccccttcg aggatcactt
    19381 ccagattgcc attggtcgac acaaaacgta catccagatg ggatgccagc ggtaccagcg
    19441 catcttccga cgcaacatca acatggtgat gtaatgccgc caatgccagt acgcgataaa
    19501 ttgcacccga gtccagcaga tgccattgca acgcttccgc catagcctta cacaaggtgc
    19561 ctttccctgc accgcttggg ccatcaatgg taataaccgg ggcaattgcc gtcatcttta
    19621 tctccttaaa acaggcgtac cgttaacata accgcgcgca ttatacgcgc cgacgccaca
    19681 aatcgttaac gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg atttattgcg
    19741 caattatagc gggctggcag aagaccagcc cgaaagatga caatgattta cttaccagag
    19801 gcgatacgat cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg
    19861 gactgggtgc gaccgccatc caggctagcc agtttttcaa agctgccaac cagcccctgg
    19921 gtgctgatgc cacgtttctt caacagatca aaagagaaat catctgcttc ggactcttta
    19981 tcacgggagt atttagcatt aatagcgcct tctgcgatat cacccaattg tgagcgggaa
    20041 agctgggaag ccacaccgct ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa
    20101 gaagctttca tttcagccag cgagtgaccc aacgcgacgt ggcccagttc atggcccaga
    20161 acaccttcaa tttcgttgtc attcatcatg tccatcaggc cactgtagac acgaacacag
    20221 ccgttcgcca tcgcccatgc gttgacgtcg ctggtcatat aaaccttata gttgaccggc
    20281 gtgccattaa tattgttacc caatgctttg gcgatttttg ccagacgttt accgtacttg
    20341 ctggatttac ttgcgacttg attgccgctg tccatttcgg cacagccctg attggcaatc
    20401 gcttttgcat cggcatcgct taatgttgct gctttatagg catttaaacc ggagctgata
    20461 gccatattgg tatcaatacc gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc
    20521 gcaatcgcca gcagtaattt agtattcttc atgtgaatca tccgttgatt aatcatatat
    20581 aaatattatg tatcaacaac ttacattgac aagcgacgct accattaatc aaaatgactc
    20641 acaaggtccg aaaaaaaacg ccgacaatgt gc
//
LOCUS       DAETPZ010000068        20551 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.068, whole genome shotgun sequence.
ACCESSION   DAETPZ010000068 DAETPZ010000000
VERSION     DAETPZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.068"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            245..586
                     /gene="raiA"
                     /locus_tag="K0W59_002800"
     CDS             245..586
                     /gene="raiA"
                     /locus_tag="K0W59_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBI3023771.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    689..814
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            690..737
                     /gene="pheL"
                     /locus_tag="K0W59_002801"
     CDS             690..737
                     /gene="pheL"
                     /locus_tag="K0W59_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HBI3023772.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            836..1996
                     /gene="pheA"
                     /locus_tag="K0W59_002802"
     CDS             836..1996
                     /gene="pheA"
                     /locus_tag="K0W59_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HBI3023773.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="K0W59_002803"
     CDS             complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="K0W59_002803"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HBI3023774.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="K0W59_002804"
     CDS             complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="K0W59_002804"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HBI3023775.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4451..4816
                     /locus_tag="K0W59_002805"
     CDS             4451..4816
                     /locus_tag="K0W59_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HBI3023776.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISD
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            4966..5484
                     /locus_tag="K0W59_002806"
     CDS             4966..5484
                     /locus_tag="K0W59_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HBI3023777.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5474..6699
                     /gene="dgcN"
                     /locus_tag="K0W59_002807"
                     /pseudo
     CDS             5474..6699
                     /gene="dgcN"
                     /locus_tag="K0W59_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6715..7197
                     /locus_tag="K0W59_002808"
     CDS             6715..7197
                     /locus_tag="K0W59_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBI3023778.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7274..7621)
                     /gene="rplS"
                     /locus_tag="K0W59_002809"
     CDS             complement(7274..7621)
                     /gene="rplS"
                     /locus_tag="K0W59_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBI3023779.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7663..8430)
                     /gene="trmD"
                     /locus_tag="K0W59_002810"
     CDS             complement(7663..8430)
                     /gene="trmD"
                     /locus_tag="K0W59_002810"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBI3023780.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8461..9009)
                     /gene="rimM"
                     /locus_tag="K0W59_002811"
     CDS             complement(8461..9009)
                     /gene="rimM"
                     /locus_tag="K0W59_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBI3023781.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQRAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9028..9276)
                     /gene="rpsP"
                     /locus_tag="K0W59_002812"
     CDS             complement(9028..9276)
                     /gene="rpsP"
                     /locus_tag="K0W59_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBI3023782.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9413..10774)
                     /gene="ffh"
                     /locus_tag="K0W59_002813"
     CDS             complement(9413..10774)
                     /gene="ffh"
                     /locus_tag="K0W59_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBI3023783.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10941..11732
                     /locus_tag="K0W59_002814"
     CDS             10941..11732
                     /locus_tag="K0W59_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HBI3023784.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11753..13039
                     /locus_tag="K0W59_002815"
     CDS             11753..13039
                     /locus_tag="K0W59_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBI3023785.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13094..13687)
                     /gene="grpE"
                     /locus_tag="K0W59_002816"
     CDS             complement(13094..13687)
                     /gene="grpE"
                     /locus_tag="K0W59_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBI3023786.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13810..14688
                     /gene="nadK"
                     /locus_tag="K0W59_002817"
     CDS             13810..14688
                     /gene="nadK"
                     /locus_tag="K0W59_002817"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HBI3023787.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14774..16435
                     /gene="recN"
                     /locus_tag="K0W59_002818"
     CDS             14774..16435
                     /gene="recN"
                     /locus_tag="K0W59_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBI3023788.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16584..16925
                     /gene="bamE"
                     /locus_tag="K0W59_002819"
     CDS             16584..16925
                     /gene="bamE"
                     /locus_tag="K0W59_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HBI3023789.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16987..17277)
                     /locus_tag="K0W59_002820"
     CDS             complement(16987..17277)
                     /locus_tag="K0W59_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HBI3023790.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17267..17704)
                     /gene="ratA"
                     /locus_tag="K0W59_002821"
     CDS             complement(17267..17704)
                     /gene="ratA"
                     /locus_tag="K0W59_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HBI3023791.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17875..18357
                     /gene="smpB"
                     /locus_tag="K0W59_002822"
     CDS             17875..18357
                     /gene="smpB"
                     /locus_tag="K0W59_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBI3023792.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18572..18934
                     /gene="ssrA"
                     /locus_tag="K0W59_002823"
     tmRNA           18572..18934
                     /gene="ssrA"
                     /locus_tag="K0W59_002823"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19139..20380
                     /locus_tag="K0W59_002824"
     CDS             19139..20380
                     /locus_tag="K0W59_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBI3023793.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRQCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAAARGSMIEGGIKGMKLVG"
BASE COUNT         5302 a         5367 c         4827 g         5055 t
ORIGIN      
        1 cccttgagct gccgtttttt tattctgtta gttgtgaaac tgaagcgatt tagtcactat
       61 cgatctcatc aaatatggct cgctttgaga tattcctcaa gtaaaaaaac acctcttcct
      121 gcgatttctc acaaaaaaga ttcgttgaca aaaagtgaca aaattatgag atttccatca
      181 cacattttga catcaggaac ggtatgctga attcaccaag acgggaagac aagaggtaaa
      241 atttatgaca atgaacatta ccagcaaaca aatggaaatt actccggcca tccgccaaca
      301 tgtcgcagac cgtctcgcca aactggaaaa atggcaaaca catctgatta atccacatat
      361 cattctgtcc aaagagccac aagggtttgt tgctgacgcc acaatcaata cacctaacgg
      421 cgttctggtt gccagtggta aacatgaaga tatgtacacc gcaattaacg aattgatcaa
      481 caagctggaa cggcagctca ataaactgca gcacaaaggc gaagcacgtc gtgccgcaac
      541 atcggtgaaa gacgccaact tcgtcgaaga agttgaagaa gagtagtcct ttatattgag
      601 tgtatcgcca acgcgccttc gggcgcgttt tttgttgaca gcgtgaaaac agtacgggta
      661 ctgtactaaa gtcacttaag gaaacaaaca tgaaacacat accgtttttc ttcgcattct
      721 tttttacctt cccctgaatg ggaggcgttt cgtcgtgtga aacagaatgc gaagacgaac
      781 aataaggcct cccaaatcgg ggggcctttt ttattgataa caaaaaggca acactatgac
      841 atcggaaaac ccgttactgg cgctgcgaga gaaaatcagc gcgctggatg aaaaattatt
      901 agcgttactg gcagaacggc gcgaactggc cgtcgaggtg ggaaaagcca aactgctctc
      961 gcatcgcccg gtacgtgata ttgatcgtga acgcgatttg ctggaaagat taattacgct
     1021 cggtaaagcg caccatctgg acgcccatta cattactcgc ctgttccagc tcatcattga
     1081 agattccgta ttaactcagc aggctttgct ccaacaacat ctcaataaaa ttaatccgca
     1141 ctcagcacgc atcgcttttc tcggccccaa aggttcttat tcccatcttg cggcgcgcca
     1201 gtatgctgcc cgtcactttg agcaattcat tgaaagtggc tgcgccaaat ttgccgatat
     1261 ttttaatcag gtggaaaccg gccaggccga ctatgccgtc gtaccgattg aaaataccag
     1321 ctccggtgcc ataaacgacg tttacgatct gctgcaacat accagcttgt cgattgttgg
     1381 cgagatgacg ttaactatcg accattgttt gttggtctcc ggcactactg atttatccac
     1441 catcaatacg gtctacagcc atccgcagcc attccagcaa tgcagcaaat tccttaatcg
     1501 ttatccgcac tggaagattg aatataccga aagtacgtct gcggcaatgg aaaaggttgc
     1561 acaggcaaaa tcaccgcatg ttgctgcgtt gggaagcgaa gctggcggca ctttgtacgg
     1621 tttgcaggta ctggagcgta ttgaagcaaa tcagcgacaa aacttcaccc gatttgtggt
     1681 gttggcgcgt aaagccatta acgtgtctga tcaggttccg gcgaaaacca cgttgttaat
     1741 ggcgaccggg caacaagccg gtgcgttggt tgaagcgttg ctggtactgc gcaaccacaa
     1801 tctgattatg acccgtctgg aatcacgccc gattcacggt aatccatggg aagagatgtt
     1861 ctatctggat attcaggcca atcttgaatc agcggaaatg caaaaagcat tgaaagagtt
     1921 aggggaaatc acccgttcaa tgaaggtatt gggctgttac ccaagtgaga acgtagtgcc
     1981 tgttgatcca acctgatgaa aaggtgccgg atgatgtgaa tcatccggcg ctggattatt
     2041 actggcgatt gtcattcgcc tgacgcaata acacgcggct ttcactctga aaacgctgtg
     2101 cgtaatcgcc gaaccagtgc tccaccttgc ggaaactgtc aataaacgcc tgcttatcgc
     2161 cctgctccag caactcaatc gcctcgccga aacgcttata gtaacgtttg attaacgcca
     2221 gattacgctc tgacgacata atgatgtcgg cataaagctg cggatcctga gcaaacagtc
     2281 gcccgaccat cgccagctca aggcggtaaa tcggcgaaga gagcgccaga agttgctcaa
     2341 gctgaacatt ttcttctgcc aggtgcagcc cgtaagcaaa agtagcaaag tggcgcagag
     2401 cctgaataaa cgccatattc tgatcgtgct cgacagcgct aatacgatgc aaccgagcgc
     2461 cccagacctg aatttgctcc agaaaccatt ggtatgcttc cggcttacgt ccatcacacc
     2521 agaccacaac ttgctttgcc aggctaccgc tgtccgggcc gaacatcggg tgtaacccca
     2581 gtaccgggcc atcgtgcgcc gccagcatgg cctgtaatgg tccatttttc actgatgcca
     2641 gatcaaccag aatacaatct ttcggtaaag gcggtaattt gccgataact tgctcagtaa
     2701 cgtggattgg cacactaaca atcaccattc cggcatcggc aacaatatca gccgctcgat
     2761 cccagtcatg ttgctccaga atccgcacct gataacccga tagtgtcagc atcttctcga
     2821 acaggcgtcc catctgaccg ccaccgccga cgataaccac cggacgcagt gacggacaaa
     2881 gtgttttaaa tcctttgtcg ttttcactgg agtaagattc acgcatcacc cgacgcaaaa
     2941 catcctcaat cagatctggc ggtacaccca gagcttccgc ctctgcacga cgcgaggcca
     3001 acatagatgc ctcgcgctcc ggaacataaa taggcagtcc aaagcggctt ttcacctcgc
     3061 ccacttcagc aaccagttcc agacgcttcg ctaataaatt cagcagcgct ttatcgactt
     3121 catcaatttg atcgcgtaat gcagtcaatt cagcaaccat aataaacctc ttaagccacg
     3181 cgagccgtca gctgcccgtt cagatcctga tgaatttcac gcagcaaggc atcggtcatt
     3241 tcccagctaa tgcaggcatc ggttacggat acaccgtatt tcatttcact gcgcggttgc
     3301 tcagaagact gattgccctc gtggatatta ctttcgatca tcagaccaat aattgagcga
     3361 ttgccatctt tgatttgagc aaccacggat tctgccaccg caggctgacg gcgataatct
     3421 ttattggaat taccgtggct gcaatctacc atcagagacg ggcgcagtcc cgcctgttcc
     3481 atctcttttt cacattgcgc aacatccgca gggctatagt tcggcgcttt accaccgcgc
     3541 aggatcacat gcccgtccgg attcccctga gtttgtagca acgcaacctg ccctgcctgg
     3601 ttaatgccaa caaaacagtg cggctgggcg gcggcgcgca tagcattaat tgctgttgcc
     3661 agactgccgt cggtgccgtt tttaaaacca accggcatgg aaagcccgga ggccatttca
     3721 cggtgagttt gtgattccgt tgtacgagca ccaattgctg accagctaaa cagatcgccc
     3781 aggtattgcg ggctattcgg atctaacgct tccgtcgcca gtggcagtcc catattcacc
     3841 agctcaagca acaatttacg cgcgatctgc agcccggctt ctacatcaaa agagccatcc
     3901 atatggggat cgttaattaa ccctttccag ccgacagtgg tacggggttt ttcaaaatag
     3961 acgcgcatta ccagatagag gctatcgctg acctctgcgg caagagcttt aaatcgacga
     4021 gcatattcca gagcagtttc cggatcatga atggaacaag gaccacatac taccagcaga
     4081 cgaggatcgc gcccggcgat aatatctgaa atggttttac gcgagtcagc aatctgggct
     4141 tcttgttgca ggctcaatgg aaaagcggcc ttcagttgtt ccggagtcat taaaacctgt
     4201 tcgtcggtaa tatgtacgtt attcagcgcg tctttatgca tgatggcgat cctgtttatg
     4261 ctcgtttgcg atagttgatc ctcagcgagg atgacgtaac gataacacat aaagtaaagt
     4321 tttcaatcca tatttcgtac atttttattt acacaggcaa tttagtcgcg ctttcaaccc
     4381 ttgcttctgt atagataaat ttacaccccc tttgaaaaca atccgctatg ctttgaaaaa
     4441 ggagaaagaa atgatgaaaa agtttatcgc ccccttgttg gctttactgg ttagcggatg
     4501 tcagattgat ccttatactc acgcgccaac cttgaccagc accgactggt atgatgtcgg
     4561 tatggaagat gcgatatcgg acagcgccat aaaagatgac gatgcattta gcgattcaca
     4621 ggcggatcgc ggtctatacc ttaaaggata tgccgaaggg caaaagaaaa cttgccagac
     4681 cgattttact tatgcccggg gactttccgg taaaagcttt cctgccagct gcaacaatgt
     4741 tgaaaatgcc agccaactgc atgaagtatg gcaaaaaggg gctgatgaaa atgccagcac
     4801 aatacgatta aattaattaa aaccccacaa ataaattaaa cataagattt tactcattgt
     4861 gttttatttc tcacattgat gacggtcgca tgaaaaaatg atattttaat gagcatcctg
     4921 aaaaaatatc gatgcatttc gagcgaagat ggtggggatc cctgaatgcg tttttctcac
     4981 cgactgttct tacttctgat tctcctgctg acgggagcac caatactcgc tcaagagcct
     5041 tctgacgttg cgaaaaacgt gcgtatgatg gtttccggta ttgtcagtta cacccgctgg
     5101 cctgcgctat cagggccacc aaagttatgt atcttttcat cttcacgttt tagtaccgcc
     5161 ctacaagaga atgccgcgac atctttgcct tatctgcctg tcatcattca cactcaacaa
     5221 gaggcgatga tttcaggctg caatggtttt tattttggca acgagtcacc gacattccag
     5281 atggaattaa ccgaacaata tccgtcaaag gcgttattat taattgccga acaaaatacc
     5341 gagtgcatta ttggtagcgc cttttgtctg atcatccaca ataatgacgt cagatttgcc
     5401 gtaaacctgg atgccttatc gcgtagcggt gtaaaggtca acccggatgt cttaatgctc
     5461 gcacggaaga aaaatgatgg ataacgataa ttctcttaat aagcgcccca cgtttaaaag
     5521 agcattacgc aacatcagta tgaccagcat atttatcact atgatgttga tctggttgct
     5581 gctttccgtg acctcggtgc tgaccctgaa acagtacgcg caaaaaaacc tggcactgac
     5641 agcagcaaca atgacttaca gtctggaagc agctgtcgtt tttgccgatg gccctgcagc
     5701 aactgaaaca ctggcagcgc tgggccagca agggcatttt caactgcaga agtacgtgat
     5761 aagcagcaaa atattctggc atcctggcat tacccccgta aggatccagg cgatactttc
     5821 agcaatttca taagccactg gctcttccct gcccccatca ttcagccgat tcgtcacaat
     5881 ggtgaaacca ttggcgaagt acgcttaacc gctcgcgaca gttcaattag ccattttatc
     5941 tggttttcgc tcgccgtact gaccggttgt attctgctgg catcaggcat cgcaattacc
     6001 ctcacccgcc atttgcacaa tggcctggtg gaagcactga aaaatatcac cgatgtcgta
     6061 catgatgtgc gttccaaccg caatttttcc cgacgagttt cggaagaacg tatcgctgag
     6121 tttcaccgct tcgctctcga cttcaacagt ctgctggatg aaatggaaga gtggcagctt
     6181 cgtttacagg ccaaaaatgc gcagcttctg cgtaccgcgc tacatgaccc attaaccggg
     6241 ctggctaacc gcgcagcgtt tcgtagcggc atcaatacgt tgatgaacaa ttccgatgcc
     6301 cgaaaaacgt cggcgttact atttcttgat ggcgataatt tcaaatatat caatgatacc
     6361 tggggtcatg cgacgggcga tagggtcttg attgaaatcg caaaacggtt agctgaattt
     6421 ggcgggctgc gacataaagc ataccgcctg ggcggcgatg aattcgctat ggtgctctat
     6481 gatgtacagt cggaatctga agtgcagcag atatgctcag cactgacaca aatctttaat
     6541 ctcccgtttg atcttcataa tggccatcag accaccatga cattaagcat tggttacgcg
     6601 atgaccattg agcacgcctc tgcggaaaaa ttacaagagc ttgccgatca caatatgtat
     6661 caggccaaac accagcgtgc cgaaaagctg gtgagataac aaggatatat cgatatgata
     6721 aagcacctgg tagcacccct gattttcacc tcactaatac tgactggctg ccagtcccct
     6781 cagggaaagt ttactcctga gcaagtcgcc gctatgcaat cttatggatt tactgaatcc
     6841 gccggcgact ggtcgctggg cttatcagat gccattctgt tcgcaaaaaa tgactacaaa
     6901 ttgctcccgg aaagccagca acagatccaa accatggcag ccaaattggc ctcgacaggg
     6961 ctaacacatg cccgtatgga tggacacacc gataactatg gtgaagacag ttacaacgaa
     7021 ggcttatcat tgaaacgggc gaatgtcgtg gccgatgcat gggctattgg tggacaaatt
     7081 ccacgcagca atctcaccac acagggttta ggaaaaaaat atcccatagc cagtaacaag
     7141 accgcccagg gccgcgccga gaaccgccgc gtcgcagtgg tgattacaac cccttaaaac
     7201 acgagcaaac agacaaaaaa agggccagcc aattggccag cccttcttaa caggatgtcg
     7261 cttaagcgaa atcttagtta agacgctctt tgatacgagc agccttacca gtacgctcac
     7321 gcaggtagta cagtttagct ttacgaacag caccacgacg tttgacagaa atgctgtcaa
     7381 ctaccggaga gtgagtctgg aagacacgct caacgccttc gccgttggaa attttacgaa
     7441 cagtgaatgc agagtgcaga ccgcggttac gaatagcgat aaccacgccc tcgaatgcct
     7501 gcagacgttt tttggaacct tcaacaaccc atactttcac ttccacggta tcacccggac
     7561 ggaaggaagg tacgtcctgc ttcatctgct cttgttcaag ttgcttaata atgttgctca
     7621 taatttaatc tcttatcctg ggtaaactga tctctcgggg gcttacgcca tcccatcatg
     7681 tttatgttgc tgttgtgcgt gttccgtttt gaactccgcc agcaaccttg cttgctcttc
     7741 agtcagagcc aggttttcca gaagttcagg tcttctaagc caggtacggc ccagcgactg
     7801 tttcaaacgc cagcgacgta tctcggcatg gttgcccgac agtaacactg gcggaacttc
     7861 catcccttct aacacctcag gccgcgtata gtgcgggcaa tccagcaatc cttcagcaaa
     7921 ggaatcttcc gttgccgagg cttcatgtcc cagtactccc ggaataaacc gggaaacgga
     7981 gtcaatcagc gtcattgctg gtaactcacc accactgaga acgtaatcgc cgattgacca
     8041 ttcttcgtca atttcggttt ggatcacgcg ctcatctata ccttcgtagc gaccgcacac
     8101 cagaatcaat ttttgattcg ttgccagttc gctgacgccc gcttgatcaa gcttgcgtcc
     8161 ctgtggtgac agataaatca cctttgcgcc ttcacccgcc gcggcttttg ctgcatgaat
     8221 ggcgtcccgc aagggttgca ccatcattaa catccccggt ccgccgccgt aaggacgatc
     8281 gtccacggta cggtgccggt catgcgtgaa gtcgcgagga ctccaactct ggatgctcag
     8341 caggccattt ttaactgccc ggccagttac cccgtaatcg gtaattgcgc ggaacatttc
     8401 aggaaacagg ctaattatgc caatccacat agcgccgtct tttaccgttt atccggtggt
     8461 ttaaaaacca ggatcccaat ctacttcgat tgaacgagta gtgagatcga ctttcttgat
     8521 aacctgccca tcgaggaacg gtacgagacg ttccttgata ccaaacgcat ctttcaggtt
     8581 tgccttaatg acgagaacgt cattagatcc ggtttccatc atatcgacga ctttaccgag
     8641 atcgtagcct tcagtggtta ctacctggca gcccatcagg tctttccagt agtagtcccc
     8701 ctcttcaagc tgaggcagct gcgatgaatc cacgacaatt tcacaattcg tcagcaggtt
     8761 cgccgcatca cgatcgtcaa cgcctttcag cttgatgatc atgtcctgat tgtggtgctt
     8821 ccagctttcc agctggactt gctgccactg acccgccctc tggataaacc agggctgata
     8881 gtcaaaaatg ctttcggcgt cttcggtgga agaaaacact ctgagccacc cacgaatacc
     8941 gtaagacgaa cccatttttc ccaaaacgat gggatcaaca ggtgcttgcg cggtgagttg
     9001 tttgctcatc atgaccaccg tgacagatta agctgctttg tttacttctt tgatcagcgc
     9061 agcaacgcga tcagaaatag ttgcgccctg gccaacccag tgagcgatgc gatccagatc
     9121 taggcgagtg ccttcttctt tttcgctagc gattgggttg aagaaaccaa cgcgctcgat
     9181 gaagcgaccg ttgcgtgcat tacggctgtc agcgacaaca acctggtaga acggacgctt
     9241 tttagcgccg tgacgtgcta aacgaatagt taccataaca tcctcttgtg tgaataaaac
     9301 aaccggaccc catcgaggaa cggagtccgg tgtcatatta aaagcccgaa aattttactg
     9361 atttctggag aaaatgcaat cagcagttaa taactctgtc gcataaggtc atttagcgac
     9421 caggaaatcc cggcggcatc attcccttca tgcttctcat catcttcgcc attccgcctt
     9481 tcttcatttt cttcatcatg cgctgcatgt cgtcgaactg cttcagaagg cggttaacgt
     9541 cctgtacctg catcccgcaa ccggcggcga tacgacgctt acgcgaacct ttgatgattt
     9601 ctggcttagc gcgctctttc atcgtcattg agttgatgat ggcttccata cgcaccagca
     9661 ctttatcgtc catctgcgac ttgacgttat ccgggatctg ccccatgccc ggcagtttgc
     9721 ccatcaagct ggccatgccg cccatatttt tcatctggcg cagttgctca agaaagtcgt
     9781 tgagatcgaa gccgtcgcct ttttttagct tgctggctaa tttctctgcc tgcgcgcggt
     9841 caactttgct ttcgatatct tcgatcagcg acagtacgtc gcccatgccg agaatacgcg
     9901 acgcgatgcg gtccggatgg aacggctcca gcgcctcagt tttctcgcca acaccgagga
     9961 acttgatcgg tttgccagtg atgtgacgaa tagagagcgc cgcaccgccg cgggcatcgc
    10021 cgtccacttt ggtcaacact acgccggtaa gcggtaacgc ttcattgaat gcttttgccg
    10081 tattggccgc atcctgaccg gtcatggcgt caaccacaaa cagggtttca accgggttaa
    10141 tcgacgcatg gacttgtttg atctcgtcca tcatcgcttc gtcaacgtgc agacgaccag
    10201 cggtatccac cagcagcacg tcgtagaatt tcagtttggc ttctttcagc gccgcgttaa
    10261 caatatcaac cggcttctga ccaacatcag aagggaagaa atcaacgccc acctgctctg
    10321 ccagcgtctc aagctgtttg attgccgccg ggcgatagac gtcagcagaa accaccagca
    10381 ctttcttctt gtgcttctcg cgcaggaaat taccgagttt accaacgctg gttgttttac
    10441 cggcaccttg caggcctgcc atcagcacga ccgcaggcgg ttgcgcagcc agattcaggg
    10501 tctggttctc ttcgcccatc gccgcaacca gttcgttacg gacgattttg acgaactcct
    10561 gccccggcgt caggctctta ttaacttcat gaccaaccgc tttctctttt acgcgattga
    10621 taaactcacg cactaccggc agagcgacgt ccgcctccag cagcgccatg cgcacttcgc
    10681 gcagcgtatc ttttacgttg tcttcagtga ggcgtccacg gccactgata ttgcgcagcg
    10741 tgcgcgacaa acgatcggtt aaattatcaa acattgtctc tcgcctgggg tggaaacggt
    10801 tggccgcaat cgcgacacat catcagtatt ttgccgcagt ataacatgaa ggcgtctttg
    10861 ttgttatgca acggttggag cagcgttcac ctgacgctat actgcttctc tttcttattg
    10921 ctcaaactgt cgacattact atgcccgttt ttgctctgct cgcgcttgtc gcctactccg
    10981 tcagtcttgc gctgattgtt cccggtctgc tgcaaaaaaa cggcggctgg cggcgcatgg
    11041 ctattatttc tgcggtcatt gcgctggtct gccacgcaat cgctctggaa gcccgcatcc
    11101 tgcccgacgg tgatagcgga caaaacctca gcctgctgaa cgttggttca ttggtcagtt
    11161 tggtgatctg tacggtaatg accattgtgg cttctcgcaa tcgtggctgg ttgctgctac
    11221 ccattgtcta tgcctttgcg cttatcaacc tggcgctggc gaccttcatg cccaatgaat
    11281 acatcaccca tctggaagca acgcctggga tgctggtgca cattggctta tcgctctttt
    11341 cctatgccac gctaattatc gccgccctgt acgcgctgca actggcgtgg attgattacc
    11401 aactgaagaa caagaagctg gcatttaacc aggaaatgcc gccattgatg agtatcgagc
    11461 gtaaaatgtt ccacatcacg cagattggcg tggtgctgct aacgctcacg ctttgcactg
    11521 gcctgttcta catgcacaac ctatttagca tggaaaatat cgacaaggct gtgctctcta
    11581 tcgtggcgtg gtttgtctat attgtgctgc tgtggggaca ttaccatgaa ggatggcgtg
    11641 gacgccgcgt cgtctggttt aacgttgcgg gcgcggtaat tctgacactg gcctacttcg
    11701 gcagccgaat tgtccagcag ttaatcagct aaacccagaa aaggagtttc ccctggaaca
    11761 catttctact actacgttga tcattattct gatcatcatg gtggtcattt cagcctattt
    11821 ttccgggtcc gaaaccggaa tgatgaccct caaccgctat cgtcttagac atatggcgaa
    11881 acagggtaat cgctcggcca aacgcgtcga aaaattgctg cgtaagccag accgcctgat
    11941 aagcctggtg ttaatcggca ataacctggt caatattctt gcctccgcgc tcggcactat
    12001 tgtcgggatg cgtttgtacg gcgatgcggg cgtggcaatt gcgactggtg tgctgacttt
    12061 tgtcgtactg gtttttgctg aggtattgcc gaaaaccatt gccgcgctgt acccggaaaa
    12121 agtcgcttat ccgagtagtt ttctgctggc tccgctgcaa attttgatga tgccgctggt
    12181 ctggttgctg aatgctatca cccgtatgct gatgcgcatg atgggtatca aaaccgatat
    12241 cgtggttagc ggctctttga gcaaagaaga gttgcgcact atcgtgcatg aatcgcgctc
    12301 acaaatttcc cgtcgcaatc aggatatgct gctgtcggtg ctcgatctgg aaaaaatgac
    12361 cgttgatgac atcatggtgc cgcgcagtga aattatcggt attgatatca acgatgactg
    12421 gaaatctatt ctgcgccaac tctcccactc acctcacggg cgcatcgtgc tttaccgtga
    12481 ttcgctggac gacgccatca gtatgctgcg agtacgtgaa gcctggcggt tgatgtcgga
    12541 gaaaaaagag ttcaccaaag aaaccatgct gcgcgccgcg gacgagatct attttgtgcc
    12601 tgaaggtacg ccgctcagca cgcagttggt aaagtttcag cgcaacaaaa agaaagtcgg
    12661 cctggtcgtc aacgagtatg gagacattca ggggctggtg acggttgaag atattctgga
    12721 agagattgtc ggcgatttca ctacgtcgat gtcgccaaca cttgccgaag aggtcacgcc
    12781 gcaaaacgac ggttcggtga ttatcgatgg caccgccaac gtgcgggaaa tcaacaaagc
    12841 ctttaactgg catctaccgg aagatgatgc ccgcacggtt aatggcgtca ttcttgaggc
    12901 actggaagag atccctgtcg caggcacccg cgtgcgtatt ggcgagtacg atatcgatat
    12961 tctcgacgta caggacaata tgattaagca ggtaaaagtt tttcctgtga aaccgctgcg
    13021 cgagagtgtg gcggagtaac gaaaacggcc ctgcattcgc atgcagggcc gtgaattatt
    13081 acgaaaacag aaattacgct tttgctttcg ctacagttac catcgccgca cgaatcgtac
    13141 gaccattcag cgtataaccc ttctgcataa tgcccagtac gttacctggc gcaacgtcat
    13201 cagattccac cattgcgata gcctgatgca cattcgggtc cagcggcacg ttagtttcgg
    13261 cgatcacttc aacaccaaac ttacgcacaa catccagcat cgacttcagc gtcagctcaa
    13321 tgccttcaac catcgcagac atatccgggt tagctttatc agccacttcc agcgcacgat
    13381 ccaggctatc aatcaccggc agcaattcgt tgatgaattt ctccagcgcg aatttgtggg
    13441 ctttttcaat atccagttca gtacgacgac gcaggttttc catttcggct tttacgcgca
    13501 aaataccgtc acgttcacgg gtctgggctt cagccagctg agcttcgaga ttcgcaattt
    13561 tttcatcgcg cggatccacc tgctcagcag aagcttctgg ctcaactgcc tcaatctctt
    13621 cgtgctgatc catgataatt tcttccgggg cttgcccctc aggcgttttc tgttctttac
    13681 tactcatgaa tttctccgcg tttttttcgc attcatctcg ctaacttcgc ttattatggg
    13741 gatcagtttc agggtttcaa gggaagcact cacattgtca tcaatcttcg caacaaggac
    13801 ctcggaaaaa tgaataatca tttcaagtgc attggcattg tgggacaccc acggcaccct
    13861 actgcactga caacacatga aatgctctac cgctggctgt gcacaaaagg ttacgaggtc
    13921 atcgttgagc aacaaatcgc tcacgaactg caactgaaga atgtgaaaac tggcacgctc
    13981 gcggagattg ggcaactggc tgatctcgcg gtagtcgttg gtggcgacgg taatatgctg
    14041 ggcgcggcgc gcacgctcgc ccgttacgat attaaagtta ttggaatcaa ccgtggcaac
    14101 ctgggtttcc tgactgacct tgaccccgat aacgcccagc aacaattagc cgatgtgctg
    14161 gaaggtcact acatcagcga gaaacgtttt ttgctggaag cgcaagtctg tcagcaagat
    14221 tgccaaaaac gcatcagcac cgcgattaac gaagtggtac ttcatcctgg caaagtggcg
    14281 catatgatag agttcgaagt gtatatcgac gagatctttg cgttttctca gcgatctgat
    14341 gggctaatta tttcgacgcc aacaggctcc accgcctatt ccctctctgc gggcggtcct
    14401 attctgaccc cctctctgga tgcgattacc ctggtgccca tgttcccgca tacgttgtca
    14461 gcacgaccac tggtcataaa cagcagcagc acgatccgtc tgcgtttttc gcatcgccgt
    14521 aacgacctgg aaatcagttg cgacagccag atagcactgc cgattcagga aggtgaagat
    14581 gtcctgattc gtcgctgtga ttaccatctg aatctgattc atccgaaaga ttacagttat
    14641 ttcaatacat taagcaccaa gctcggctgg tcaaaaaaat tattctaatt ttacgccagc
    14701 ctctttactg tatataaaac cagtttatac tgtacacaat aacagtaatg gtttttcata
    14761 caggaaaacg actatgttgg cacaactgac catcagcaac tttgctatcg ttcgtgagct
    14821 tgagattgat tttcatagcg gcatgaccgt aataactggc gagaccggtg cgggtaaatc
    14881 tattgcaata gatgccctcg gtctttgtct cggtggtcgc gctgaagccg acatggtgcg
    14941 taccggcgct gctcgcgctg acctgtgcgc ccgtttttct ctgaaagata cgccagcggc
    15001 cctgcgctgg ctggaagaaa accagcttga agacgggcat gaatgtttgc ttcgtcgcgt
    15061 gatcagcagt gatggtcgct cccgtggttt catcaacggt acagctgttc ctctgtcaca
    15121 actgcgcgaa ctgggtcagt tgctgattca gatccatggt cagcacgctc atcaattact
    15181 caccaaacct gagcaccaaa aattcctgct tgatggctat gccaatgaaa cctctctact
    15241 tcaggaaatg accgcacgtt atcagttgtg gcatcaaagc tgccgtgacc tcgcgcatca
    15301 tcaacagtta agtcaggaac gcgccgcccg tgcggaactg ctgcaatacc aattaaaaga
    15361 acttaacgaa tttaatccgc agcccggaga gtttgagcaa atcgacgaag agtacaaacg
    15421 tctggcgaac agcggtcaat tgctgaccac cagccagaat gcattggcat taatggccga
    15481 cggtgaagac gcaaacctgc aaagtcagct ttacacggct aaacaactgg tgagcgaatt
    15541 gattggcatg gacagcaaac tgtccggcgt acttgatatg ctggaagaag ctaccatcca
    15601 gattgctgaa gccagcgacg aactgcgcca ctactgcgat cgtctggatc tcgatcccaa
    15661 ccgactgttt gaacttgaac agcgcatctc aaaacagatt tcgctggcac gtaaacatca
    15721 cgtcagccct gaggcattac cacagtatta ccagtcgcta ctggaagaac agcagcaact
    15781 ggacgatcag gccgactcac aagaaacgct cgcgctggcg gtaacgaaac atcatcagca
    15841 ggcactggaa accgcgcgcg cattacacca acaacgccag caatatgcag aagaacttgc
    15901 acagctgatc accgacagta tgcatgcgct ctcaatgccg catgggcagt ttacgattga
    15961 tgtcaaatat gacgagcatc acctgggcgc tgacggtgcc gatcgtattg agtttcgggt
    16021 aaccaccaac ccaggtcagc caatgcagcc tattgccaaa gtcgcatccg gtggtgaatt
    16081 gtcccgcatc gcactggcaa tccaggtcat cacggcgcgt aaaatggaaa ccccggcact
    16141 gatttttgat gaagtggatg tagggattag cggtccaaca gcggcagttg tcggcaaact
    16201 gctgcgtcaa ctcggcgaat caactcaggt gatgtgtgtt acccacctgc cacaagtcgc
    16261 gggatgtggt catcaacact attttgtcag caaagaaacc gatggtgcga tgacagaaac
    16321 acatatgcaa tctctggata aaaaagcgcg gttacaagag ctggcgcgcc tgcttggtgg
    16381 cagtgaagtc acacgtaaca cactggcgaa tgcgaaagaa ctgcttgcag cgtaaacttt
    16441 tttcctgctt cacggtcaga gtaaacagca aaacgccgta agaccggaaa gcaaaaggtt
    16501 ttaaagtgat gaaaggtcta ttatcatcgg catattacag atgagccacg tactgctcgg
    16561 gcccgaaaag gaatcaaatc tctatgcgct gtaaaacgct gactgctgca gcagcagtac
    16621 tattgatgtt gaccgcaggc tgttccactc tggagcgagt ggtttaccgt cctgacatca
    16681 accaggggaa ctatctgacc gctaacgacg tatccaaaat acgtgttggc atgacgcaac
    16741 aacaagttgc gtacgcattg ggtacaccgc tgatgtccga tccatttggt acgaatacct
    16801 ggttctatgt cttccgccag caaccaggtc atgaaggtgt aacgcagcaa acgctgacgc
    16861 tgacctttaa cagtagcggt gtgttgacca atattgataa caaacctgcg ctgagtggta
    16921 actaataata aagttgctct caaagacgtt aaaaaaggtg ctcaatgagc accttttttc
    16981 tgtctgttat ttattcgctg atttttctgc tcgttgcctg cgaagctctt tcggatcggc
    17041 aatgagaggg cgataaatct ccacccgatc gccatcatgc acactatcgc ttagttttgc
    17101 cggacggctg taaatgccga ctttgttttt agttaaatcg atatcggtac gcaattccag
    17161 caagccactg gcgcgaatag cttcttcaac cgtcgcgccc tcctgcagcg tcactcgctg
    17221 caggtactgc ttctcaggta gcgcataagc cacctcaacg gcaattttac ctagcactgt
    17281 aaacctcttt cgcacgaacc gtaaaagcct ggaccatatt agccgccagc tctttaaaca
    17341 cgcgaccaaa ggcgagttca atcaacttat tggtaaactc aaagtcgaga tgaaactcga
    17401 tacgacacgc ctcctggctc agcggcgtaa acttccatcc accaatcaat ttcttgaacg
    17461 gcccatccac cagattcata agaatacttt ggttactggt caactggttg cgggtagtaa
    17521 acgttttgct gatcccagcc ttagagacat ctaccgccgc agtcatctgc ccaggagtgg
    17581 actccagaat ccgacttccg gtacaacctg gcaaaaactg aggataagac tgaacgtcat
    17641 tcactaactg atacatttgc tccgcgctgt agggtaccag tgcggtccgg ctaatctgag
    17701 gcataacaat ttccatcaac aaaaatccaa caaataatat catttatcct gctgtaaaaa
    17761 aaacgctatc ccggcgctgg gtaacatcgg gttcatgcta agatagagcc ttgtcccccg
    17821 caggattgat atggggtgtt ttcgatttca gattaccgat gattcacgac gcttatgacg
    17881 aagaaaaaag cacataaacc tggttcagcg accatcgcgc ttaacaagcg cgcccgtcac
    17941 gaatacttta tcgaagaaga gttcgaagcg ggacttgccc tgcaaggctg ggaagttaaa
    18001 tccctgcgcg caggaaaagc caatatcagc gacagctacg tccttctgcg tgacggagag
    18061 gcatttctgt ttggcgctaa catcacgcca atggccgtgg cctccacgca tgtggtgtgc
    18121 gatcctaccc gtacccgcaa gttacttctc aaccagcgcg aactggactc attgtacggt
    18181 cgcgtcaatc gagaaggcta taccgtagtg gcgctctccc tgtactggaa aaatgcctgg
    18241 tgcaaagtga aaatcggcgt cgccaaaggt aaaaaacagc acgataaacg ttcagatatc
    18301 aaagaacgcg aatggcaggt ggataaagca cgcatcatga aaaacgccca ccgttaaacc
    18361 tgcactccaa ttattgacca gttcctcacc gcgcctccct ctccggcggc gcgaatgaac
    18421 atcttattgg ctatcacatc cgacacaaat gttgccatcc cattgcttaa tcgaataaaa
    18481 atcaggctac atgggtgcta aatctttaac gataacgcca ttgaggctgg tcatggcgct
    18541 cataaatctg gtatacttac ctttacacat tggggctgat tctggattcg acgggatttg
    18601 cgaaacccaa ggtgcatgcc gaggggcggt tggcctcgta aaaagccgca aaaaatagtc
    18661 gcaaacgacg aaaactacgc tttagcagct taataacctg cttagagccc tctctcccta
    18721 gcctccgctc ttaggacggg gatcaagaga ggtcaaaccc aaaagagatc gcgtggaagc
    18781 cctgcctggg gttgaagcgt taaaacttaa tcaggctagt ttgttagtgg cgtgtccgtc
    18841 cgcagctggc aagcgaatgt aaagactgac taagcatgta gtaccgagga tgtaggaatt
    18901 tcggacgcgg gttcaactcc cgccagctcc accaaaattc tccatcggtg attaccagag
    18961 tcatccgatg aagtcctaag agcccgcacg gcgcaagccc tgcgggcttt tttgtgcccc
    19021 gaatttgtcc cgcgaagtct gatgccaact aattaaatcc gatcctttta ggcaccttgt
    19081 taggcacttc ataaagctat attgtctttg aggtgcctaa aactatggaa acccggcaat
    19141 ggcaagacaa accaaacctc tatccgttaa ggaaatcgaa tctgctaaac ccaaggaagc
    19201 ggactacgtt ctctatgatg gcgatggcct tgagctactc attaaatcca gcgggagtaa
    19261 aatctggcag tttcgctaca ttcgccctgt caccaagaca cgagcaaaga aaagcatagg
    19321 cccttacccg tcagttaccc ttgccgatgc cagaaactac cgtgcagagt cccgctcact
    19381 cctagctaaa cagattgatc cgcaggaaca tcagcaagaa caattgcgta gttcgctgga
    19441 agccaaaacc aatactttcc agctcgtagc tgaacgttgg tggaatgtga agaaagccag
    19501 tgtgacagag gactatgcgg aagatatctg gcgctctctt gaaagagatg tctttcctgc
    19561 gattggcgac gttagcgtta cagatattaa agctcataca ctggttcagg ccgtacaacc
    19621 ggttcaggcc agaggagcac tggaaacggt tcgtcgccag tgtcaacgta ttaatgaggt
    19681 catgatctat gcccagaaca cagggctgat tgatgctgtt cccagcgtta atatcggtaa
    19741 agccttcgaa aagcctcaga aaaagaacat gcccagcatt cgaccggatc agctatctca
    19801 actgatgcag acaatgcgaa cagccagcat tagcctttcc acacgctgcc tgttcatgtg
    19861 gcaacttctt actattaccc gccctgccga agcggctgaa gctcgctggg aagaagtaga
    19921 catagaagcg caagagtgga agattcccgc agcacgcatg aaaatgaacc gcgaccatac
    19981 tgttccattg tcagatgaag caatagctgt gctggagatg atgaaaccgc taagcggcaa
    20041 ccgagaattt atctttccta gccgtatcaa gcccaaccag cctatgaaca gtcagactgt
    20101 taacgcctca ctaaaacgcg caggttttgg tggtgtgctt gtttcgcatg gtctgagatc
    20161 aattgccagt acggctctta atgaacaagg ctttccgcct gatgccattg aggcagcatt
    20221 ggcccatgtg gataagaatg aggttcgtcg tgcttataac cgcagcgatt acctggaaca
    20281 gcgtcgcccg atgatgcaat ggtgggcaaa ttttgtgatg gcagcggctc gtggaagcat
    20341 gattgaaggt gggataaaag gaatgaagct agttgggtga ctaaatagct gcgcggcata
    20401 gtgtcccact ttcagccagc ccttgcaggt taagccacca gacagtaaac ggcaggtgtc
    20461 gcgttattag gcgcagctat ttagtagtaa tattaggatt ggtgattctg cactccagaa
    20521 gttcggtaat gctgccaact tactgattta g
//
LOCUS       DAETPZ010000069        19944 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.069, whole genome shotgun sequence.
ACCESSION   DAETPZ010000069 DAETPZ010000000
VERSION     DAETPZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19944
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.069"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..455
                     /gene="feaR"
                     /locus_tag="K0W59_002825"
     CDS             <1..455
                     /gene="feaR"
                     /locus_tag="K0W59_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HBI3023794.1"
                     /translation="VHLIQESMNNPALSETESEAALQAMVCLLRPVLHQRESVQPRRE
                     RQFQKVVTLIDDNIREEILRPEWIAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFC
                     ADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(627..956)
                     /locus_tag="K0W59_002826"
     CDS             complement(627..956)
                     /locus_tag="K0W59_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HBI3023795.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(961..1146)
                     /locus_tag="K0W59_002827"
     CDS             complement(961..1146)
                     /locus_tag="K0W59_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HBI3023796.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(1143..3782)
                     /locus_tag="K0W59_002828"
     CDS             complement(1143..3782)
                     /locus_tag="K0W59_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HBI3023797.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLDVKVDNWQAGLENALVSGRLSVLTQGQAGKGNVVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHVGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3990..4979
                     /gene="ldhA"
                     /locus_tag="K0W59_002829"
     CDS             3990..4979
                     /gene="ldhA"
                     /locus_tag="K0W59_002829"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBI3023798.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAVLELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            5090..5426
                     /locus_tag="K0W59_002830"
                     /pseudo
     CDS             5090..5426
                     /locus_tag="K0W59_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
     gene            complement(5423..5689)
                     /locus_tag="K0W59_002831"
     CDS             complement(5423..5689)
                     /locus_tag="K0W59_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HBI3023799.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5963..9487
                     /gene="nifJ"
                     /locus_tag="K0W59_002832"
     CDS             5963..9487
                     /gene="nifJ"
                     /locus_tag="K0W59_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBI3023800.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAGAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9855..10984
                     /gene="ompN"
                     /locus_tag="K0W59_002833"
                     /pseudo
     CDS             9855..10984
                     /gene="ompN"
                     /locus_tag="K0W59_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            11125..11559
                     /gene="uspF"
                     /locus_tag="K0W59_002834"
     CDS             11125..11559
                     /gene="uspF"
                     /locus_tag="K0W59_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HBI3023801.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11735..12670
                     /gene="ttcA"
                     /locus_tag="K0W59_002835"
     CDS             11735..12670
                     /gene="ttcA"
                     /locus_tag="K0W59_002835"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBI3023802.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(12802..14169)
                     /gene="dbpA"
                     /locus_tag="K0W59_002836"
     CDS             complement(12802..14169)
                     /gene="dbpA"
                     /locus_tag="K0W59_002836"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HBI3023803.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(14647..15630)
                     /gene="zntB"
                     /locus_tag="K0W59_002837"
     CDS             complement(14647..15630)
                     /gene="zntB"
                     /locus_tag="K0W59_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HBI3023804.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            15885..17117
                     /gene="dgcM"
                     /locus_tag="K0W59_002838"
                     /pseudo
     CDS             15885..17117
                     /gene="dgcM"
                     /locus_tag="K0W59_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(17138..17701)
                     /gene="smrA"
                     /locus_tag="K0W59_002839"
     CDS             complement(17138..17701)
                     /gene="smrA"
                     /locus_tag="K0W59_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HBI3023805.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(17955..19409)
                     /locus_tag="K0W59_002840"
     CDS             complement(17955..19409)
                     /locus_tag="K0W59_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HBI3023806.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGILQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(19406..19801)
                     /locus_tag="K0W59_002841"
                     /pseudo
     CDS             complement(19406..19801)
                     /locus_tag="K0W59_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         5239 a         5057 c         4700 g         4948 t
ORIGIN      
        1 cagtacacct catacaggag agcatgaata atccggcact ttctgaaaca gaaagtgaag
       61 ctgcgctaca ggcgatggtg tgtctgcttc gcccagtact tcatcagcgg gaatctgtcc
      121 aacctcgccg tgaacgtcag tttcaaaaag tggttacgtt gatagacgat aatattcgcg
      181 aagagatatt acgcccggag tggatagccg gagagacagg tatgtcagta cgtagtttgt
      241 accgaatgtt tgccgataaa ggtttggtag tcgcgcaata tattcgtaac cgtcgtctcg
      301 atttttgtgc agatgcgatt cgccatgccg cagatgatga aaaactggca ggcatcggct
      361 ttcattgggg attttctgac cagagtcatt tctcaacggt atttaagcaa cgctttggga
      421 tgacgccagg cgagtatcgc cgtaaattcc gctaactaat tggcgttgca tgtactgcaa
      481 cgccaattag ttaaaacaga tatttgacta tgcatttttc agggcgaaag gcaatttgac
      541 aggagttttt gaatattcac cttgtttcag gaaaatagat caatcgcttt ctcctgtagc
      601 gttatcccga tattgcggtt ttgctattaa aactttcgca cccatttacc gtttatccct
      661 tgtacatatt cccccggttt ggcccgagct acgagctttt gcccggcgag tttcgcgata
      721 tcatctacag acacattatt ttgctttgcc cgttgttggt aactcgcttt acgttcggca
      781 ttaatttcgg ttactaattt ctctgtttca gcatccgttt tcagcgcaac cagataacca
      841 taaaatgttt cacctacccg cccctgagtt ctggcttcat ccagagtcaa tgccattaca
      901 ttgctgctta ccagcccaac aagaaacgca caaagaagta atgttttctt catcatcgcc
      961 tcagaaaaga tcgctacgag tttcaagcag ctcttcgaca tctttgtctg ccttgatgat
     1021 gatctcatgc tcaattttaa cgttcatatt gatagtgatc ggttccttag gtgcagcgac
     1081 ttcaatgcga ggcgtacagc caaccagcat aaaagatgac gtcaacgcag ccagtaaaat
     1141 tttcattgtt tttcctcaca ctcttttcct tgcggacagt catttcccgg cagacgtgcg
     1201 ttctgctcca accatgcctg gagattatcg ccaaagcgta aactgcgcca cagcgtaaaa
     1261 atattctctt catgatggta attaaggttt accgtaccgc ttttaccatc aacgcgactg
     1321 gtacctgtaa tgttggcctg catggttaat aaaccgagat tatctaagtt aatttttgtc
     1381 gatgaccggc taatttccat atagcgcaac cagttaattg ctgaacccgc agtcatattg
     1441 tctttcacca ccgcatctgc ggtatcttta tccagccgca aggtcattgg cccgctattt
     1501 gccagccaac catctttcac tatccatttt tcgttgttta gccataacgg caatgcacca
     1561 ctaaatgccc cggacatggc gaattgtttc ggattgacgg cgctaaccag ttcgctggat
     1621 gagagattat tcagccgcaa cagcgccggg tcatgttgcg gcatacgtaa ttgtttcatc
     1681 agcacgttac cgcctaacac atcgacgcta acatcagtca acagcaaggg ttcttcttca
     1741 gtccacggat aacgcccttg caaatcagcc gtaatatttt tcgctgtcac cagattaatc
     1801 acttcggcaa ttcgcaacgt aacggggccg cgagtcccca gatgccaggc tccatcggca
     1861 aaacggaaag gcaggacaaa atcgacaccg ttaacctgat tatctggcat ccaggcacta
     1921 ccgcctttca acacgccgtg ccctcccgcg cggaatcctt gttcaggcgc agcagaaaat
     1981 gcaacctgag catacagttc accatcgcgt aagttcatct tccagtcggg tggcaccagc
     2041 ggctggaata cagtcagtga ttgttttggc caccaggcgt taccgcgcag acgaataccg
     2101 tcccagcgac catttacccg aaccgggcca atctcaccaa catgtaaatc gcctttaaag
     2161 aggaaatagg tcggatcgcg cccatcgacg ctaaatttta aggttgatgg cggtaacaca
     2221 ctgccgccag tgaacagcgt ttgcccggcg tccagtgaca gcgcgccgct gaaggaggga
     2281 tgctgtgcgt cacgtaccca gacgacgggc ttgtcgagaa ttaatcgcgg cttttctacc
     2341 gtcatcgtac cgtattgtaa ctggtcgaaa ccggtggaga gatcggtcag cgtaatcgtg
     2401 ctgtcatgcc actcaccttt tcctgcgaca tcccaggtgg cattcatcgg tgtaaaactc
     2461 ccttttcccc agtagcgcca ctgccagcgg ccagcgtcag ggagaaaatc attcgccagc
     2521 ccatccatat gcagcacgaa atcgcccagt tcattttcat gcgcctgcaa aatggcctgc
     2581 aaacgtccgt caacaccacg ttgggtgact tttacacccg ctaaaggcca gcggatttca
     2641 tcgatgtcca gcgaatctat gactcttccc tttgaacgaa gtaacgcgcc tggctcaaag
     2701 gtcagcgttg ggtcagacag acttccactt agctgcgcag gtaaacgggc atataaaatg
     2761 aggtccgcct gtttcgcttc accggtcagc tgcagaggca gttgactgtt atccatgctt
     2821 aattttcctg ggccaaaatt aagcaccacg ttgcccttac ccgcttgccc ctgggtcagc
     2881 acactcagtc gtccgctgac cagagcgttc tcaagccctg cctgccagtt gtcaactttg
     2941 acatccagtc ggccactcaa aggaaaacct gcatacagcc agctccagcg accatcgctt
     3001 acggtcaatt gttgacgagt aatttgccac ggcaaatcga gcaacggatc gccgttatcc
     3061 cgtgccagca cgatcaattg cccgctattt tcctgccagt ccagctcggc atccaccagt
     3121 gacggttcct gcggcaagtt taacgtcgca gtagcatgcc cacttacagg aagtccatcc
     3181 ggcacgagcg gcatagtaaa ttcccccacc agtttaaccg gcggctgatt ttcaaacgcg
     3241 acgacatcca gttcgctgac tgtaagttgt tgccctttca gctggccttg aaatttaact
     3301 ttttcgccct gataacgcag ttgctggata tcagaggtta atgcgagaga gagttttccc
     3361 tgccattcct gccagggaga aaaaatcagt ttatcgatat tgatccaggt gttaggcagc
     3421 atggactgcc actgcgcgag ggtttttgga gcagctggcg attgctccgt ctgcggcaat
     3481 ttcgccagac aagcagaatc aagttctacc gtgccgacgt tcaataacca gcggctggga
     3541 tgtgaaaggc tggcgttggt gatatgcgca agctgacaat ctcccaccag ataacggaga
     3601 tcgggaatga ttaaaccttt acgcgtaatg cgtgggcttt catctaatgc aatacgtgtc
     3661 ccgagcggta gccagatgtc cgccagcgtg ggaacccaca gcccgagcgt catcagcagc
     3721 gtcaacggca caagaataat cagtaataac agcgcgagaa cggctttata tttacccagc
     3781 atgggtagtt aatatcctga tttcgcgaaa aattaagcat tcaatacggg tattgtggca
     3841 tgtttaaccg ttcagttgaa ggttgcgcct acactaagca tagttgttga tgaatttttc
     3901 aatatcgcca tggctttcaa ttatatttga aattttgtaa aatattttta gcagcttaaa
     3961 tgtgattcaa catcactgga gaaagtctta tgaaactcgc cgtttatagc acaaaacagt
     4021 acgacaagaa gtacctgcaa caggtgaacg agtcctttgg ctttgagctg gaattttttg
     4081 actttctgct gacggaaaaa accgctaaaa ctgccaatgg ctgcgaagcg gtatgtattt
     4141 tcgtaaacga tgacggcagc cgcccggtgc tggaagagct gaaaaagcac ggcgttaaat
     4201 atatcgccct gcgctgtgcc ggtttcaata acgtcgacct tgatgcggca aaagaactgg
     4261 ggctgaaagt agtccgtgtt ccagcctatg atccagaggc cgttgctgaa cacgccatcg
     4321 gtatgatgat gacgctgaac cgccgtattc accgcgcgta tcagcgtacc cgtgacgcta
     4381 acttctctct ggaaggtctg accggcttta ctatgtatgg caaaacggca ggcgttatcg
     4441 gtaccggtaa aatcggtgtg gcgatgctgc gcattctgaa aggttttggt atgcgtctgc
     4501 tggcgttcga tccgtatcca agtgcagcgg tgctggaact cggtgtggag tatgtcgatc
     4561 tgccaaccct gttctctgaa tcagacgtta tctctctgca ctgcccgctg acaccggaaa
     4621 actaccatct gttgaacgaa gccgccttcg atcagatgaa aaatggcgtg atgatcgtca
     4681 ataccagtcg cggtgcattg attgattctc aggcggcaat tgaagcgctg aaaaatcaga
     4741 aaattggttc gttgggtatg gacgtctatg agaacgaacg cgatctgttc tttgaagata
     4801 aatccaacga cgtgatccag gatgacgtat tccgtcgcct gtctgcctgc cacaacgtgc
     4861 tgtttaccgg gcaccaggca ttcctgacag cagaagctct gaccagtatt tctcagacta
     4921 cgctgcaaaa cttaagcaat ctggaaaaag gtgaaacctg cccgaacgaa ctggtttaat
     4981 cttgccgctc ccctgcaatc caggggagct gattcagata atccccaatg acctttcatt
     5041 ctctattctt aaaatagtcc tgagtcagaa actgtaattg agaaccacaa tgaagaaagt
     5101 agccgcgttt gttgcgctaa gcctgctgat ggcgggatgt gtaagtaatg acaaaattgc
     5161 tgttacgcca gaaatcagct ttggtgaaaa aatgatgatt tccggcagca tgtgtaaccg
     5221 ctttagcggt gaaggcaggt gaactgacag ccaaagggct ggcaatgacc cgtatgatgt
     5281 gcgctaaccc gcagcttaat gaactcgata acaccattag cgaaatgctg aaagaaggtg
     5341 cacaagtgga tctgaccgcg aaccagttaa cgctggcgac cgcgaaacag acattaactt
     5401 ataagctggc ggatttaatg aattaatagc tgccacagct cccggcggca agtgactgtt
     5461 cgctacagcg tttgccgttg ggtaatgcac acatcccaat cgccgtacca tccagttgac
     5521 gggcaacaga aagcgaaccg ccgatcattg cacaatttgc ttctccacta ctggacatcg
     5581 acgcttttaa acttggcgct acgtgcgccg cagtcgcctg ctgaacaggc tcactactac
     5641 acgccgacaa caataaagcg gcacacccta cccaaaacgc tgctcgcatc tcttcttcct
     5701 ctgatcttca agccaaacga caccgccata aataataggc agcacagagg gcggcgtcga
     5761 gagctgtcct gcgcgttgcc ccgcattttt acttttttat ggctattttt ttgccctctg
     5821 tttgatcaaa acattcatta ctctgatgtg ggggacacaa aagcgaaaat gcagaagaaa
     5881 gccatttgct aaaattgaag gattactact gggcgcgcag caatttcgtg cgcccctcat
     5941 ttgcgcaatg taagggtgtc atatgattac tattgacggt aatggcgcgg ttgcttcggt
     6001 ggcgtttcgc accagtgaag ttatcgccat ctaccctatt acccccagtt ccacgatggc
     6061 agaacaggct gatgcctggg ccggaaacgg cttgaagaac gtttggggag acacaccacg
     6121 cgtggttgaa atgcagtcgg aagcgggtgc tatcgctacc gtgcatggcg ctttgcagac
     6181 gggtgccctt tcaacatcgt ttacgtcatc gcagggtttg ctgctgatga tcccgacgct
     6241 gtacaaactg gcaggcgaac tgacgccatt tgtcctgcat gtagcggcac gtaccgttgc
     6301 cacacatgca ctttctattt ttggcgatca ttccgacgtt atggcggtgc gccagacggg
     6361 ttgcgcgatg ttgtgtgcgg ctaacgtcca ggaagcgcaa gactttgctc tcatttcgca
     6421 catcgcaacg ctgaagagcc gcgtgccatt tattcatttc tttgatggtt ttcgcacgtc
     6481 ccacgaaatc aataaaattg tcccgctggc cgatgacact attcttgatc tcatgccgca
     6541 ggccgaaatt gatgctcatc gcgcccgggc gctcaacccg gaacatccgg tgatccgcgg
     6601 tacgtccgcc aatcctgaca cttatttcca gtctcgcgaa gccaccaacc cctggtacaa
     6661 cgcggtctat gaccatgttg aacaggcgat gaatgatttc gctgccgcga caggtcgtca
     6721 gtatcagccg tttgagtatt acgggcatcc gcaagcggaa cgggtgatta tcctgatggg
     6781 ctctgccatt ggcacctgtg aagaagtggt tgatgaattg ctaacccgtg gcgaaaaagt
     6841 tggcgtgctg aaagttcgtc tgtaccgtcc cttctccgcc aaacacttgc tgcaagctct
     6901 gccgggatcc gtacgcagcg tggcggtact ggacagaacc aaagaacccg gtgcccaggc
     6961 agaaccgctc tatctggatg tgatgaccgc actggcagga gcctttaata atggtgagcg
     7021 cgaaaccctg ccccgtgtca ttggtgggcg ctatggtctt tcatccaaag aatttggccc
     7081 ggactgtgta ctggcggtat ttgccgagct caacgcggct aaacctaaag cacgctttac
     7141 ggttggtatt tacgatgatg tgaccaatct gtcactgccg ttgccggaaa acactctgcc
     7201 aaactcggcg aaacttgaag ccttgtttta tggccttggt agtgatggca gcgtttccgc
     7261 aaccaaaaac aatatcaaga ttatcggtaa ttccacgccg tggtacgcac agggctattt
     7321 tgtttacgac tccaaaaaag cgggcggcct gacggtttct caccttcgcg tgagcgaaca
     7381 gccgattcgt tccgcttatc tcatttccca ggctgatttt gttggctgcc accagttgca
     7441 gtttatcgat aaatatcaga tggctgagcg tttaaaacct agcggcattt tcctgcttaa
     7501 tacgccgtac agcgcagatg aagtgtggtc gcgcttgccg caggaagttc aggccgtgtt
     7561 aaaccagaaa aaagcgcgct tctatgtgat taacgcggcg aaaatcgccc gcgaatgtgg
     7621 cctggcggcc cgtattaata ccgtcatgca gatggctttt ttccatctga cgcaaattct
     7681 gcctggcgat agcgccctcg cagaattgca gggtgcgatt gccaaaagtt acagtagcaa
     7741 aggtcaggat ctggtggagc gcaactggca ggctctggcg ctggcgcgtg aatccgtaga
     7801 agaagttccg ttgcaaccgg taaatccgca cagcgccaat cgaccgccag tggtttccga
     7861 tgccgcccct gatttcgtga aaaccgtaac cgcggcgatg ctcgccgggc ttggtgatgc
     7921 cctccccgtt tcggcgctgc cgccagacgg cacctggccg atgggcacca ctcgctggga
     7981 aaaacgcaat atcgccgaag agatccccat ctggaaagag gaactctgta cccaatgtaa
     8041 ccactgcgtc gccgcttgcc cacactcagc tattcgcgca aaagtggtac cgcctgaagc
     8101 gatggaaaac gcccctgcca gcctgcattc gctagatgtg aaatcgcgtg atatgcgcgg
     8161 gcagaaatat gtcttgcagg tggcaccgga agattgcacc ggttgtaacc tgtgcgtcga
     8221 agtttgcccg gcgaaagacc gtcagaatcc agagattaaa gccatcaata tgatgtctcg
     8281 ccaggaacat gtcgaagaag agaaaatcaa ttacgatttc ttcctcaacc tgccagaaat
     8341 cgaccgtagc aaactggaac gtattgatat tcgtacatcg cagctgatta caccgctgtt
     8401 tgaatattca ggtgcttgcc ccggttgtgg cgagacgccg tatattaaat tgctgactca
     8461 gctctatggt gaccggatgt tgatcgctaa cgccaccggc tgttcttcaa tttatggcgg
     8521 taacctgccc tctacaccgt ataccaccga tgccaacggt cgtgggccgg catgggcgaa
     8581 ctctctattt gaagataatg ccgaatttgg ccttggtttc cgcctgacgg tcgatcaaca
     8641 ccgtgtccgc gtgctgcgtc tgctggatca atttgccgat aaaatcccgg cggaattact
     8701 gacggcgttg aaatcagacg ctacgccaga ggttcgtcga gaacaggtcg cagctttacg
     8761 ccagcaactc aacgatgttg ccgaagcaca tgaactgctg cgtgacgcag atgcactggt
     8821 ggaaaaatca atctggctga ttggtggcga cggctgggct tacgatatcg gctttggcgg
     8881 tctggatcat gtattgagtt tgacggaaaa cgtcaacatt ctggtgctgg atacgcaatg
     8941 ctattccaac accggtggtc aggcgtcgaa agcgacaccg ctgggtgcag taactaagtt
     9001 cggcgagcac ggcaaacgta aagcgcgtaa agatcttggc gtcagtatga tgatgtacgg
     9061 tcatgtttat gtggcgcaga tttctctcgg cgcgcagctg aaccagacgg tgaaagcgat
     9121 tcaggaagcg gaagcgtatc cgggaccatc gctgatcatt gcttatagcc cgtgtgaaga
     9181 gcatggttac gatctggctc tcagccacga ccagatgcgc caactcactg ctaccggctt
     9241 ctggccgcta tatcgctttg atccgcgtcg tgccgatgaa ggcaaactgc cgctggcgct
     9301 ggattcacgc ccgccgtcag aagcactgga agaaacgtta cttcacgagc agcgtttccg
     9361 tcgtctgaat tcgcagcagc cagaagtggc agaacagtta tggaaagatg ctgcagctga
     9421 tttgcaaaaa cgctatgact tcctagcaca aatggccgga aaagcggaaa aaagcaacac
     9481 cgattaaatg ctctggataa ggattatcca attctaaaaa aaaaagcccg gacgactgtt
     9541 cgggcttgtc tttttatatg ttggaaaatc agtggcaatg caatggccca acagaaaata
     9601 aaatctgtga caataaaggc atataacccg cgcagaataa cgtatagaaa attaaaagta
     9661 taatttttat tttgtatgta ttatcttcga tacattcacc agacttatta tcatttcaga
     9721 ggaattattt gattaaggtt ttacttaagg cgtaacaaat gatatttatc ggcaaactga
     9781 acttctcctt taggatgttt tcactccccg aaatggggat aaaaggcaaa taaaataact
     9841 aaaggattta ttcaatgaaa agaaaagcac tggcactttt aatccctgcc ctgctcgccg
     9901 caggtgctgc acatgcagcc gaagtttata ataaagacgg caacaaatta gatctgtatg
     9961 gcaaagttga tggcctgcat tatttttctg ataattcagc gaaagatggc gaccagagct
    10021 atgcgcgtct gggttttaaa ggcgaaaccc aaattaacga tcaacttact ggctacggtc
    10081 aatgggaata caatattcag gcaaacaaca ctgaatcttc aaaaaaccag tcatggaccc
    10141 gtctggcatt tgccggactg aaatttgcag attacggttc tttcgattac ggacgtaatt
    10201 atggcgtaat gtacgacatc gaaggctgga ccgatatgct gcctaaattt ggcggtgact
    10261 cttataccaa tgcagacaac tttatgactg gtcgagccaa tggcgtcgcg acttatcgta
    10321 atactgattt cttcggtctg gtaaatggtc tgaacttcgc ggtgcagtat caaggtaaca
    10381 acgaaggagc cagtaatggt caggaaggca ccaataacga acgcgatgtt cgtcatgaaa
    10441 acggtgacgg ctggggtctt tccacaacat atgatttagg catgggcttt agcgctggtg
    10501 cggcatacac ctcttctgac cgcaccaatg accaggttaa ccatactgcg gcgggtggtg
    10561 ataaagcaga cgcgtggact gctgggctaa aatacgatgc taacaatatt tacctagcaa
    10621 ccatgtattc agaaacgcgt aatatgaccc cgtttggcga cagcgattat gcagttgcga
    10681 acaaaaccca gaactttgaa gttactagta ccagtttgat tttggtctgc gtccggcagt
    10741 ctctttcctg atgtctaaag gccgtgacct tcacgctgct ggtggtgcag acaacccggc
    10801 aggtgttgat gataaagatc tggttaaata cgccgatgtt ggcgcgactt actatttcaa
    10861 taaaaacatg tccacctatg ttgactataa aatcaacctg ttggatgaag atgacagttt
    10921 ctacgctgcc aacggcatct ctaccgatga tattgtcgct ttaggtctgg tttatcagtt
    10981 ctaaatcctc ctgcccgctg ttatggcggg ctttttctgc ttattcttcc tcctttgatc
    11041 taaattaaaa atgtgaactc cgtcattaca caaaaagtgt catctggcgt tacactttat
    11101 gcggatacta aaacaggagg ttttatgaac agaacgattc ttgtccctat cgatatttca
    11161 gattcagaat taactcaacg cgtgattagc cacgttgagg cagaggcaaa gattgatgat
    11221 gcagaggttc atttcctgac ggtaatacct tcactgccct actatgcctc tctgggttta
    11281 gcgtattccg cagaattacc ggcaatggat gacctgaaag cggaagccaa atcgcaactg
    11341 gaagagatca ttaaaaaatt taaactgcca accgacagag tgcatgtcca tgttgaggaa
    11401 ggctcgccca aagaccgcat tctggaattg gcgaagaaga tccccgctca tatgatcatc
    11461 attgcttccc atcgaccgga tatcaccact tatctgctcg gttccaacgc cgcagctgta
    11521 gtgcgtcacg cagagtgctc cgtgctggtt gtgcgctgac actaacgccc gcacattgct
    11581 gcgggctttt tgattcattt cgcaaatgtg ctgacatttt cccctctaat ccgtaccata
    11641 cacgccacag tttttatatc agatttctta tgctgagcgt tccggcatga cgcatactct
    11701 tctgatgcca tataacgaat tgagtcgctt ttaaatgtcg caaaatcaag aaattagtaa
    11761 gaaagaacaa tacaacctga acaaattaca aaaacgtctg cgtcgtaacg tgggcgaagc
    11821 cattgctgac ttcaatatga ttgaagaagg cgatcgcatc atggtttgcc tctccggggg
    11881 taaagacagc tataccatgc tggagattct gcgcaatttg cagcaaagcg cgccaatcaa
    11941 tttttcgctg gtggctgtta acctcgatca aaagcaaccg ggcttcccgg aacacgttct
    12001 gcccgagtat cttgaaaagc tgggcgttga gtacaagatt gttgaagaga acacttacgg
    12061 tatcgtgaaa gagaagattc cagagggcaa aaccacttgc tcactgtgtt ctcggctacg
    12121 tcgcggtatc ctttatcgca ccgcaacgga actggggacg acgaagatcg cgttgggtca
    12181 ccatcgtgac gatatcctgc aaacgttgtt cttaaatatg ttctacggcg gcaagatgaa
    12241 aggtatgcct ccgaaactga tgagcgatga tggcaaacat atcgttattc gtccactggc
    12301 ctactgccgc gagaaagata ttcagcgatt tgccgatgca aaagcgttcc cgattattcc
    12361 gtgcaacctg tgcggttcac agcctaacct gcaacgtcag gtgattgctg acatgttgcg
    12421 tgactgggat aaacgttatc cagggcgtat cgagacgatg ttcagcgcga tgcagaatgt
    12481 ggtgccgtcg catctgtgcg ataccaacct gttcgatttc aaaggcatta cccacggttc
    12541 tgaagtggtt aacgggggtg atctggcgtt tgatcgcgaa gagatcccac tacaaccggc
    12601 gggctggcag ccagaagaag atgaaaatca gttggatgag ttacggctga atgtggttga
    12661 agtgaaataa ccaggatagc gcccgatgcg caagcgtatc gggctactct tatggaggcc
    12721 ggataagacg cggccagcag cgtcgcatcc ggcaatcccg aataagatgt ttactcttgc
    12781 acccggcaat tcaacatttc attattttaa taaccgcacc cggcacgttt ttcctttaat
    12841 cttcccgccc tgtaactgtt tccatgcttt atgagcaaca gcctgacgga ccgcgacata
    12901 gacatgcgcc ggatgcacgg cgattttgcc aatatctgcg ccatcaagcc cgatatctcc
    12961 tgtcagtgca cctaatacat cacccgggcg cattttggct tttttcccgc catcgataca
    13021 caacgttgcc atttctgctt ccagcgtcac aatggaacta ttagctggcg gcgtttgcca
    13081 gttaagtttt atctgcaaca tgtcagaaat gatattggcc cgctgtgctt cttccggagc
    13141 acagaaactg atcgccagac cgctatttcc tgcacgagct gtacgaccga tgcgatgtac
    13201 atgaacttca gggtcccacg ccagctcaaa gttcaccacc agctcaagcg atttaatatc
    13261 cagaccacgc gcagcaacat cagtcgcaac cagtacacgg gcgctaccgt tagcaaaacg
    13321 taccagggtc tgatcgcgtt gctccagatc gccgtgtaac gacaatgcac tttgctctac
    13381 ttcattcagc gcgtcgcaga cagcctggca atcttttttg gtattgcaaa acaccacaca
    13441 agaggatggc tgatgcaagc ttaataaccg ttgcaacaga ggaattttgc ctttactgga
    13501 tgtctcataa aattgttgtt caatgggtgg caaagcatct gttgagtcaa tttcaatcgc
    13561 caaaggatcg cgttgcactc gtccgctgat tgcggcgatg gcttccggcc aggttgccga
    13621 aaacagaagc gtctgtcgag atgcaggcgc aaaacggatg acatcatcaa tggcatcgct
    13681 aaatcccata tccagcatgc ggtcggcctc atccatcacc aaagtattca gcgcatccag
    13741 tgataccgtg cctttttgca agtgatccag caaacgaccc ggcgttgcca cgataatatg
    13801 cggcgcatgt tgcaacgaat cacgctgcat accgaacggt tgaccaccgc acaacgtcaa
    13861 aattttggta tttggcagaa aacgcgccag ccgacgcaat tcacctgcca cctgatccgc
    13921 cagttcacgc gtaggacaca gcactaaagc ctgggtttga aatagcgacg catcaatttg
    13981 ctgtaacaag ccgaggccaa aagccgccgt tttgccgctg ccggttttcg cctgcacgcg
    14041 aacatctttt ccggcaagga tcgccggaag cgcggcggcc tgcaccggcg tcatggttaa
    14101 ataacccaac tcattaaggt tcgtgagttg ggcgggaggc aaaacattca gggtagaaaa
    14161 agcggtcaca atctattctc gtggtcatcg acgcaaagtt agcaggcgcg tatcctcgca
    14221 gatctacgct cacgatgcga caatttaatc ggttcttcat cgggtggtgg gtcaggcatg
    14281 ggttgcgggc gagggatcgg atcgggcact ggaacaggat cgccaggaat cggttcaggg
    14341 acaggaattt gcaaataaat aagtgtcgtc atatttccct ccggtcattg ggtggactct
    14401 taaagggtag acgctgataa ataacaggca aaaaaaagcc gactcatcaa agtcggcgtc
    14461 gtacgaatca attgtgctat gcagtaattc aaaaaaggaa gtaagacaat atggagcgca
    14521 acgcccatcg cttgacgttg cattcacctg caagggagat attgccctga atgggtagag
    14581 agtttattga cttcgctcaa actttgcggc gtttttgtat acagacagcc ggaaaaattg
    14641 cttttgttac aaccatttac tacggtgcaa ccataaagca acaccaccaa taagaacaac
    14701 taacagaata caaaaaattg aaaatccgaa ttgccacccg ccgccaggga tcccaccaag
    14761 gttgacgcca aataacccgg tcagaaaggt actgggtaaa aagaccattg ccatcaacga
    14821 cattgtatag gtacgacgag ctaaattttc ctgcatcacc tgagcgattt catccgccat
    14881 cacgccagtc cgtgctatac aggcgtcgat ttcgtcaagg ccgcgcccaa ggcgatcggc
    14941 aatatcctgc atccgacggc gttggtcatc gctcatccac ggcaaacgtt cactggcaag
    15001 acgagcataa acatcacgtt gcggtgccat atagcgacgc atcacaatta attgtttgcg
    15061 cagcagagcc aggaatccac gcggtggaat ttgctgatca aggagattat cttcaaggtc
    15121 gataatttta tcgtgcagct gctcgataaa ttcactggaa tgatcggtca acgcatcgca
    15181 cacatccacc agccatcccc cgcaatcggt cggacccgtg ccctcttcca gatcgctcac
    15241 cacatcgtcc agcgccagca ctttgcgttg tcgggtcgaa acaattaacc gcccgtccat
    15301 atatacacgc atggcgacca gttgatcggg gcgttcatcg gtgctgccgt ttatacagcg
    15361 caatgtaatc agcgtgcctt caccgaaacg gctgactcgg ggccgtgtgc tctcgcccgc
    15421 cagcgcatca cgtacgttat tgggaagcag cggtgttgtc gccagccatt gggcgctatc
    15481 atggtgtaca taattaaggt ggagccagca gggatgcgct tcatcaatca catctgtatt
    15541 ttccagcggt ttaacgccgc ctctaccatc cagcatccag gcaaatactg catccgggac
    15601 attaacgtcc gatcccttaa tcgcttccac agtgcctcca tcatcaacgc attattttgt
    15661 agtctagcct tctggccctg ttacgcaaca tctcatcacc ccattaccct gaaatgatta
    15721 ataaaattct gtctaaattg aatacaaaaa gcaaaatgct tttccgtata caaaccgtgt
    15781 gaagtgttaa atagcgtcta tcattatcag aattatctga tcatatgacg tggctttttt
    15841 gcgatcggat agcaacaaaa attgataaaa ataacgggat ctcaatgatt acgcacaact
    15901 tcaataccct ggacttactc accagtcctg tctggattgt ttcgcccttt gaggaacagt
    15961 taatttatgc caatagcgcg gcgcgactgt tgatgcaaga cctcacgttt agtcagctac
    16021 gaaccggacc ctattccgtc tcctcacaaa aagaactgcc gaaatacctc tccgatctgc
    16081 aaaaccaaca cgatattatc gaaatcctca ctgttcagcg taaagaagag gaaacagcat
    16141 taagctgtcg gcttgttttg cgagagctga cagaaacaga accggtgatt attttcgaag
    16201 gtatcgaagc gccggcaacg ctgggtttaa aagccagtcg ctcggcaaat tatcagcgca
    16261 aaaaacaagg tttttatgcg cgcttttttc tgactaactc tgcaccaatg ttgttgattg
    16321 acccgtcacg agatggacaa atcgtcgatg ctaacctcgc cgcgctcaat ttctatggtt
    16381 ataaccatga aacgatgtgc cagaaacata cctaggaaat aaatatgctt gggcgtcgcg
    16441 tcatgcctat catgcatgaa atttcgcatt tacccggcgg tcataaccct ttgaattttg
    16501 ttcataaact ggcggatggt tctactcgtc atgtgcagac ctatgccgga ccgattgaaa
    16561 tttatggcga caagctcatg ttatgtattg tgcatgatat tactgagcaa aaacggctgg
    16621 aggagcagct ggaacatgct gctcaccatg acgcgatgac cggattactg aatcggcgac
    16681 agttttatca cattacggaa ccaggccaaa tgcagcatct cgccatcgct caggattaca
    16741 gcttgttgct catcgacacc gatcgtttta aacacattaa cgatctctat gggcattcta
    16801 aaggtgatga ggtgttatgc gccctcgccc gccccctcga aagttgcgct cgcaaaggcg
    16861 atttggtgtt tcgttgggga ggcgaagagt ttgtcttatt gctaccaaga accccactgg
    16921 ataccgcgct ttcgctggct gaaactatcc gcgtaagcgt ggcaaaagtg agtatttcgg
    16981 gcttaccacg ctttaccgtc agcattggtg tggcgcatca cgaaggaaat gaaagcatcg
    17041 atgaactgtt taaacgcgtt gatgatgctt tgtatcgggc gaaaaatgat ggacgcaacc
    17101 gcgtgctggc ggcataagcc gcggatgcgt ctcgagatca acgactgcgc ttagcgtggc
    17161 gctcccagtt ttcttgcttc gcctgcgccg ttttacgtag tgcgacgtaa cacgccccgc
    17221 tgccgccatg atgcggtagc gcggtgcaat atgcctgaac atcatcaaat tcggtcagcc
    17281 agcgcgccac atagctgcgg acaatattgg catgtgattt atcttcccgc cctttaccat
    17341 gaataatcag cacattacgc aaaccatccg ccagggcttg ttgaatgaaa ctgaacacca
    17401 ttttgcggca ctcttccacc ggctggcgca aaagattcag gctcgcctgt tgcggatatt
    17461 taccactgcg cagcttatcc agcaccccat gctgcaaccc ttcctgccga aactccagcg
    17521 gctgacttag cgggatgatg tcgagaaatc cggtagtgag gaaattatca agctgcagcg
    17581 tgtcgatacg ctgcggcgca cgttggttac gcgttggatg ccagtggaca tctgtagcac
    17641 gtttcagcgg ctggacatct tccatggcgt caagaaacag cgatttgtcg tcaaggttca
    17701 tgtaacctcc tatgcacctt cacacgtcgt gattcagacg ggttaattgt tcatacttac
    17761 tgcatcgttc ccttcacaga ttgaaacaat actctacttc atacctgtga gtatctgcgc
    17821 aggcggggca ttttatacta gcctgttagc agtatcgaac agtttagccc gagtttaatt
    17881 cagcacaggc tccgatcagg atgatttatt catagcagga accaacaaaa atgcgataaa
    17941 taacagacca ctgattagca gaaaggtacc gaaaatgcca tagtttgcgc ctccctcagc
    18001 atcaattgac tcaataattt ggttgaagcc agtatttccg atgtctggtc acttctactg
    18061 cgattaaaaa ataaagcact tctggaaatg aagtcattca ccgtatgggg aacagaaacc
    18121 attttttcac ccattctcga aaaatgaaaa ttagttcgat tattgagaat agagccacac
    18181 agggcaatac caatcgcgcc cccaagatta cgtgttaaat taaatacacc agaggctaac
    18241 ttcagccgtt cttttggaat tccccccagc gttaatgtta cgatgggggc catcgcaaac
    18301 tgctgcaaaa tccctctaat tgcctgtgga aacagtaatt cctgccatcc ccattcgtgg
    18361 gttatagggg taaaaagata cattgaaaac acaaatccgc ccagccccgc catcaacagc
    18421 catcgcagat ttattttttt acttaaccag aagtaaaagg gaacagaaaa taactggaaa
    18481 atacctgttg tgcaaacggc aaaacctatc tcttcggcat ttaatccgcg aatctgcccc
    18541 aaaaacacag gtattaaata tacagtagag aatatcccaa cccctccaga aaaagaaaaa
    18601 aaacatccca gagtgaaatt tttatcctta aaagcatgaa gatccattat cggattggat
    18661 attgtaagtg tacgcgcagc aaataaaata aaactcacca atgccagaac tgatgtcagc
    18721 aaaatagtat tatcatccag ccatccccag cgcgctcctt cttcaagggt atattccaga
    18781 catcccagag tcatggccaa caggataata ctcggataat cagcaacttt cagtaaagag
    18841 agatcaggtt tatcaaaatt aaccagaaaa ggaatactca aaactaaata tatgccaggt
    18901 aatatattga tataaaatag ccatcgccag tcgaggttat cagtgagcca gccccccaga
    18961 gttggcccta gcgtcggtga cagtgaggcc agcgcactga ctaccgcagc agccaacccc
    19021 aactctttac cctggtaata aataaacgcc gtcgtgaaaa ctaaagggat catggatgcg
    19081 ccagcagctc cctgtaaagc acgaaacaaa atcatgatct gaatattcca tgcaagtcca
    19141 cacgcgatac tcattaaagt aaatattccc gcggatagag taaaaagcca acgagtggaa
    19201 aatacccgtg acagccaccc agagagcgga ataacaataa tctcagcaat taaatagctg
    19261 gtctgaagcc aggccatttc gtctttacct gcggacagac caccacctat ttcatttagg
    19321 gatgacgata caatttgaat atcaagctga gcaataaata atcccataca catactggcg
    19381 aaagcaatat gtttacgcat agacatcatc tctcatcaac actgacggtg acggacagtc
    19441 ccgggcgaag aagttgcttt aattcctttg cgtcgtcaaa tgcaatccgt accgggacac
    19501 gctgaacaat tttggtgaaa ttgccagtgg cattttctat cggaatcaag ctgaaactgg
    19561 ctcctgtcgc tggggaaagg ctttcaatgt gaccgtgaaa cacttcccct ttgagaatat
    19621 cagcacggat ctcagccttc attcctggct tcattcccgc tatttggttt tctttataat
    19681 ttgcatcaac ccacaactca ctgactggta ctagtgaaac taacgatgtg ccgccttcca
    19741 cccaactgcc tgtatgggcg ctgcggtttg cgacaatgcc atcaatcggc gcacgaacca
    19801 cggcagcctg acggctgcaa attgaaaacc gcccctgttc gtcctgttgt tgccgcacgc
    19861 tccgcagaat atgtatgctc tgaggacctg ttttattaag gactcatcct cttatgttga
    19921 cccgcaaaag tatagatacc gttc
//
LOCUS       DAETPZ010000070        19918 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.070, whole genome shotgun sequence.
ACCESSION   DAETPZ010000070 DAETPZ010000000
VERSION     DAETPZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.070"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..748)
                     /locus_tag="K0W59_002842"
     CDS             complement(<1..748)
                     /locus_tag="K0W59_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3023807.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADSDTDSY
                     MWFETGDNGNEYFKWRHHLTGGRVKDLMNLKWDALYVLVKALFSSEVKISTVNALRIF
                     NSSFGAIFRRSEECLHIIPTRENEGENGNIGPLRPFTLNLRTGRISMGHGLVVTGDIF
                     TNRFLINSSTGMWIHMRDQNVIMGRNAVSNDGAQALLRQDHTDRKFVIGGLGNRQFGI
                     YMINNSRTANGTDGQAYMDNNGNWLCGSQVI"
     gene            842..1039
                     /locus_tag="K0W59_002843"
     CDS             842..1039
                     /locus_tag="K0W59_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI3023808.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTRTDRPG
                     LKRALKRLQKGTHIENDDRIN"
     gene            1133..1321
                     /locus_tag="K0W59_002844"
     CDS             1133..1321
                     /locus_tag="K0W59_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI3023809.1"
                     /translation="MKSIRCKNCNKLLFKADSFDHIEIRCPRCKRHIIMLNACEHPTE
                     KHCGKREEITHSDETVRY"
     gene            1272..1967
                     /locus_tag="K0W59_002845"
     CDS             1272..1967
                     /locus_tag="K0W59_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HBI3023810.1"
                     /translation="MGKEKKSRILTKPCVIEYEGQIVGYGSKELRVETISCWLARTII
                     QTKHYSRRFVNNSYLHLGVFSGRDLVGVLQWGYALNPNSGRRVVLETDNRGYMELNRM
                     WLHDDMPRNSESRAISYALKVIRLLYPSVEWVQSFADERCGLAGVVYQASNFDFIGSH
                     ESTFYELDGEWYHEITMNAIKRGGQRGEYLRANKERAVVHKFNQYRYIRFLNKRARKR
                     LNTKLFKVQPYPK"
     gene            complement(2079..2426)
                     /locus_tag="K0W59_002846"
                     /pseudo
     CDS             complement(2079..2426)
                     /locus_tag="K0W59_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2395..4052)
                     /gene="dpiB"
                     /locus_tag="K0W59_002847"
                     /pseudo
     CDS             complement(2395..4052)
                     /gene="dpiB"
                     /locus_tag="K0W59_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            4433..5491
                     /gene="citC"
                     /locus_tag="K0W59_002848"
     CDS             4433..5491
                     /gene="citC"
                     /locus_tag="K0W59_002848"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBI3023811.1"
                     /translation="MFVNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKLRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWFHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            5506..5802
                     /gene="citD"
                     /locus_tag="K0W59_002849"
     CDS             5506..5802
                     /gene="citD"
                     /locus_tag="K0W59_002849"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBI3023812.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            5799..6707
                     /gene="citE"
                     /locus_tag="K0W59_002850"
     CDS             5799..6707
                     /gene="citE"
                     /locus_tag="K0W59_002850"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBI3023813.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            6718..8250
                     /gene="citF"
                     /locus_tag="K0W59_002851"
     CDS             6718..8250
                     /gene="citF"
                     /locus_tag="K0W59_002851"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBI3023814.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            8254..8805
                     /gene="citX"
                     /locus_tag="K0W59_002852"
     CDS             8254..8805
                     /gene="citX"
                     /locus_tag="K0W59_002852"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBI3023815.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            8780..9658
                     /gene="citG"
                     /locus_tag="K0W59_002853"
     CDS             8780..9658
                     /gene="citG"
                     /locus_tag="K0W59_002853"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBI3023816.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            9709..11172
                     /gene="citT"
                     /locus_tag="K0W59_002854"
     CDS             9709..11172
                     /gene="citT"
                     /locus_tag="K0W59_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HBI3023817.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMDKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNML
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            11286..12092
                     /gene="rna"
                     /locus_tag="K0W59_002855"
     CDS             11286..12092
                     /gene="rna"
                     /locus_tag="K0W59_002855"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HBI3023818.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            12321..12731
                     /gene="rnk"
                     /locus_tag="K0W59_002856"
     CDS             12321..12731
                     /gene="rnk"
                     /locus_tag="K0W59_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HBI3023819.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLRDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(12962..14199)
                     /locus_tag="K0W59_002857"
                     /pseudo
     CDS             complement(12962..14199)
                     /locus_tag="K0W59_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            14420..14848
                     /gene="uspG"
                     /locus_tag="K0W59_002858"
     CDS             14420..14848
                     /gene="uspG"
                     /locus_tag="K0W59_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HBI3023820.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(14969..16534)
                     /gene="ahpF"
                     /locus_tag="K0W59_002859"
     CDS             complement(14969..16534)
                     /gene="ahpF"
                     /locus_tag="K0W59_002859"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBI3023821.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(16779..17342)
                     /gene="ahpC"
                     /locus_tag="K0W59_002860"
     CDS             complement(16779..17342)
                     /gene="ahpC"
                     /locus_tag="K0W59_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HBI3023822.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            17714..18460
                     /gene="dsbG"
                     /locus_tag="K0W59_002861"
     CDS             17714..18460
                     /gene="dsbG"
                     /locus_tag="K0W59_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HBI3023823.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            18670..19572
                     /locus_tag="K0W59_002862"
     CDS             18670..19572
                     /locus_tag="K0W59_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3023824.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            19719..19918
                     /locus_tag="K0W59_002863"
                     /pseudo
     CDS             19719..19918
                     /locus_tag="K0W59_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         5020 a         5127 c         4758 g         5013 t
ORIGIN      
        1 gaataacttg cgaaccgcaa agccagttcc cgttattatc catgtacgcc tgaccatcgg
       61 tgccattggc tgtccttgag ttattaatca tgtagatgcc aaattgccta ttccccagcc
      121 caccaattac gaatttacgg tcggtatggt cctggcgaag caatgcctgc gcaccatcgt
      181 tggataccgc attacggccc ataataacgt tctggtcacg catatgaatc cacatgcctg
      241 tactgctgtt aattaaaaaa cggtttgtaa atatatctcc tgtaacaacc agaccatgcc
      301 ccatgcttat ccggccagtt ctgagattaa gcgtaaaggg gcgtagtggc cctatattac
      361 cattttcccc ttcattctct cgtgtaggga tgatatgcag gcattcttca gaacgacgaa
      421 aaatggcacc aaaagatgaa ttaaatattc tcagtgcatt gaccgtcgat atttttactt
      481 cactgctgaa aagggcttta acaagaacat acagagcatc ccatttaaga ttcatcaggt
      541 ctttaacccg gccgccagtg aggtggtgtc tccatttgaa atactcattg ccgttgtcgc
      601 ctgtttcaaa ccacatgtat gaatcagtat cgctgtcggc atcattttta aatccaatct
      661 ttgcccagtc agtattccga atccaggcaa ggattgagtc gttttcaaaa gtaagtccac
      721 cggacaacgt atcgccattt ttttgcacgg cgttaccagc cttgtttacc gtttcctgta
      781 aaccgagggg ccgtttctgg cctgcatggc atgatttccg cttttaaacg gggaattcag
      841 cgtgctgatt ggctatgtaa gggtatcaac aaatgaccag aatacagacc tgcaacgaaa
      901 cgctcttgtt tgtgcaggat gtgaacaaat atttgaagat aaattaagcg gaacaaggac
      961 agaccgaccg ggattaaaac gtgctttaaa gcgccttcaa aaaggaacgc atatagaaaa
     1021 cgacgatcga atcaattaaa tcgatctata ttaccgatcg attatgtccc gataaccaca
     1081 cccgactgat gagatttttt aagataacgg cgaattgatg caaggaggtg aaatgaaatc
     1141 aattcgctgt aagaactgca acaaactgtt atttaaagcg gattcctttg atcacattga
     1201 aatcaggtgt ccgcgttgca aacgtcacat cataatgctg aatgcctgcg agcatcccac
     1261 ggagaaacat tgtgggaaaa gagaagaaat cacgcattct gacgaaaccg tgcgttattg
     1321 agtatgaagg ccagattgtt ggctatggtt caaaggagct gcgcgttgaa accatatcct
     1381 gctggctggc ccgcacaatc attcagacaa agcactattc ccgccgtttt gtgaataact
     1441 cttaccttca cctgggagta ttcagcgggc gtgatctggt tggcgttctc cagtggggat
     1501 atgcccttaa ccccaactca ggtcgtcgtg tcgtgcttga aacggataac cggggctata
     1561 tggagttgaa tcgcatgtgg ctacacgacg acatgccccg caactctgaa tcacgggcca
     1621 tcagctacgc gctgaaagtt atcagattac tgtatccgtc agtggagtgg gttcagtcct
     1681 ttgctgacga acgctgcgga ctcgcaggcg ttgtgtatca ggcgtcgaat tttgatttta
     1741 ttggcagcca tgaaagcacg ttctacgagc tggatggtga atggtatcac gagataacga
     1801 tgaacgcgat taagcgcgga gggcaacgag gcgagtattt gcgagctaat aaggagcgtg
     1861 ccgtggtgca caaatttaat cagtatcgct acatcagatt cctgaacaag cgagcaagga
     1921 agcggctaaa taccaaacta ttcaaggttc agccataccc taagtgatcc ccatgtaatg
     1981 aataaaaagc ggtaattaat actgctgttt catttgaagc gcgaaagcta aagttttcgc
     2041 atttatcgtg aaacgctttc gcgtttttcg tgcgccgctt cacccagccg ttcataggca
     2101 attggcttaa tgagataatc aaatacacca caacgtacgg cttcagacac cgtttccata
     2161 tcactggctg cagtggtaaa caccacgtcg ccgggataat gcgcctgcac cagttcatgc
     2221 agtaaattaa tccctctacc gtcaggaaga tagttatcga gcaagattag ccccggctta
     2281 aaacgctcga tcatcattcg ggcctgcgcc aggtttcccg ccagtaatat ctgactgaat
     2341 ccgggaatgt gacgaatata ttccgcatgc atctctgcca gcggcgtttc gtcctcaacg
     2401 atcaataggg ttaatggagc tgtcattagg tttcactttc ggaatataga ttgaaaataa
     2461 ggtaccgcag ggatcattat cttcgagagt gataacacca ccgcagcgcg ttacgtagct
     2521 ggcaatcaag tacaacccaa tgccatgttc accgggctcg tcagcacgcg tactgacccc
     2581 tgctcaaata ttttgtctcg tagagactct ggaacgccgc agccctgatc ggcgacttca
     2641 atcaccacat catcgccttc atcgctgagg aataattcaa cgctcttgtt tccttcatcg
     2701 ctacgcaggc tggcttcgaa ggcgttatca agtaaattgc caacaatggc ggcaaactcg
     2761 gtgctatcca gtcctggcgg cagttgcgaa agctggctac cggggacgat gatcattttt
     2821 agccccagtt cgcgggcgcg ctgcacttta ccaaaaagca gccccgccac ctggcgatcg
     2881 gcaaacgcct cgcgcaggct gtcaataagc tgttgctggg cctgagactc cccctgcacc
     2941 atcgccagca cgcgatcata ctctttcatc tgcaacagac cattgagagt cgacatccaa
     3001 ttgagatgct cgtgtcgcaa tgtacgaagg ctctcaacgt attgttttat ttgcgtcagt
     3061 tgcgcattga gggtggaaat ttcgtcttta ctgcgaaagc tgataatggc tcccagcaaa
     3121 tcatcacctg aacgaatagc ttcccgattg gcaataacgc tcagaccgtt aaagttagcc
     3181 accacatcct gacgtttttc atcaatctgt tcggtaaaga aatcggcaga cctgaccact
     3241 tcagcaatgg gtttacccaa ccattgccgt ccaggggaac tcagccccag catctttctt
     3301 gcgttacgat tgatggcggt aatgtaaccg tgcggatcca ccgcaatcag cccttcataa
     3361 accgaactaa acagcgcctc ttgctgacgg accacccgtg cgatttgctt tggctccatg
     3421 cccatcatct gccgacggat atgcgcggcc aggaaccacg acagcaacat cagaatccct
     3481 aacagcacga caaacacacc tgccatcggt aataaaaact cagcccgcca gctatcgatt
     3541 ttactcacca ggtagccaat cgacaccacg ccgatgactt ttccatcgtc atcaaagatt
     3601 ggcgttttgg cgcgcatcgc catacccatt gaccctttcc cggtaatgaa gtagctctcc
     3661 cctttctcca gcgcgccctg tttggtgaac tgcataggat aaccaatttt ctccggatta
     3721 ggatggtaaa ggcggatcga gtgccggtcg ccaatcacca cataatcaaa atcggtatct
     3781 ctttgtaatt tgttagcgat ggtcgccagc cgtttgtagt cacgcgtttt caccgcagag
     3841 atgacactgt cattggaggc aataattttc gcctgattca ttgccatgtc gcgtacatga
     3901 agcgttaaat agtcctcaaa actggccgta aaatattgcg ccagggctgc aatgacaaat
     3961 attgagaaca ccagaatcag caaaaagata cgcagcggaa atgtcagtct ttggaaaaat
     4021 gcaaactgtt tattctcgtt aagctgcaac attgttttcc ttgcgggttg tcccgagcct
     4081 ctttgacgga tgtaattaaa atgttgaatc aaaatacata gatattaatt atgatcgtgg
     4141 caaattttat tcattaaatc aattaaatca cttaaacctg cgagtcattt cgtaattaca
     4201 ttaattaaaa aacttaaaac cattaaacaa ataaaaaaac cactaaccct atgtgaaata
     4261 aatcaaaatt tcacgccgaa atactcctta ggatgtatag cgaaaggaga aaaagatata
     4321 cctcgatcac acccctttct cccaagtgaa aataaaaggt tatcagtttg caacattgaa
     4381 caacattcgt tgcaaatcga taacaacatg caccttcagg atactattta ttatgttcgt
     4441 caatgatatt ttcacccgcg taaaacgttc agaaaataaa aaaatggcgg aaatcgccca
     4501 attcctgcat gaaaatgatt tgagcgttga caccacagtc gaagtattta ttaccgtaac
     4561 ccgcgatgaa aagcttatcg cgtgcggtgg aattgccgga aatattatta aatgcgttgc
     4621 tatcagtgaa tccgttcgcg gtgaaggact ggcgctgaca ttagccactg aactgataaa
     4681 cctcgcctat gagcggcaca gcacgcatct gtttatttat accaaaaccg aatacgaggc
     4741 gctgttccgc cagtgcggtt tttccacgct gacctgcgta cccggcgtga tggtgctgat
     4801 ggaaaacagc gccacgcgac tgaaacgcta tgccgaatcg ctgaaaaaat tgcgtcatcc
     4861 agggaacaag attggctgca ttgtgatgaa cgccaatccc tttacgaatg gtcaccgtta
     4921 tctgattcaa caagctgcgg cacagtgcga ctggtttcat ctgtttttag tcaaagaaga
     4981 ttcttcacgc ttcccctatg aagaccggct ggatctggtg ttaaaaggca ccgccgatat
     5041 tccacgcctg actgtgcatc gcggctcaga atacatcatc tcccgcgcta cgttcccttg
     5101 ctacttcatt aaagaacaga gcgtcattaa ccattgttac accgaaattg atctgaagat
     5161 tttccgtcag tacctcgctc ccgcgcttgg tgtaactcac cgctttgtcg gtactgaacc
     5221 cttttgtcgc gttaccgccc agtacaacca ggatatgcgc tactggctgg aaacgccgac
     5281 tatctccgca gcgcccatcg aactggttga aattgagcgg ctgcgttacc aggagatgcc
     5341 gatatccgct tcccgggtac gtcaactgct ggcgaaaaac gatctcacgg ctatcgcgcc
     5401 gctggtccct gcagtcacgc tgcattattt gcagaacctg cttgaacact cccgccagga
     5461 cgcggcagct cgtcaaaaga cccccgcatg agaaacaggt gaaaaatgaa aataaaccag
     5521 cccgccgttg caggcaccct tgagtctggg gatgtgatga tacgcatcgc cccactcgat
     5581 acgcaggata tcgacctgca aatcaatagc agcgttgaga aacagtttgg cgatgcaatt
     5641 cgcaccacca ttctggacgt tctcgcccgc tacaacgtgc gcggcgtaca gctgaatgtc
     5701 gatgacaaag gcgcactgga ctgcatttta cgtgcacgac tggaagccct gctggcacgc
     5761 gccagcggta tcccggctct gccatgggag gattgccaat gatttccgct tcgctgcaac
     5821 aacgtaaaac tcgcacccgc cgcagcatgt tgtttgtgcc tggtgccaat gccgcgatgg
     5881 tcagcaactc cttcatctac ccggctgatg ccctgatgtt tgacctcgaa gactccgtgg
     5941 cattacgtga aaaagacacc gcccgccgca tggtttatca cgcgctgcaa catccgctgt
     6001 atcgcgatat tgaaaccatt gtgcgtgtca acgcgctgga ttccgaatgg ggtgttaacg
     6061 acctggaagc cgtcgttcgc ggtggtgcgg acgttgtgcg tctgccgaaa accgataccg
     6121 ctcaggatgt tctggatatt gaaaaagaga tcctgcgtat agaaaaagcc tgtggtcgtg
     6181 aacccggcag caccggcctg ctggcggcga ttgaatctcc gctgggcatt acccgcgcag
     6241 tggaaatcgc tcacgcttcc gagcgtttga tcggtatcgc cctcggtgca gaagactatg
     6301 tgcgcaacct gcgtacagaa cgctccccgg aaggaactga actgctgttc gcacgctgtt
     6361 ccattttgca ggccgcgcgc tctgcgggta ttcaggcgtt cgataccgtc tattccgacg
     6421 ctaacaacga agccggattc ctgcaagaag ccgcccacat caaacagctg ggctttgacg
     6481 gcaaatcgct gatcaacccg cgtcagattg atctgctgca caacctctac gcaccgaccc
     6541 agaaagaagt ggatcacgcc cgccgcgtcg tagaagccgc tgaagccgcc gctcgcgaag
     6601 gcctcggcgt ggtttccctg aacggcaaga tggtggacgg tccggttatc gatcgcgccc
     6661 gtctggtgct ctcccgtgca gaactttccg gcatccgcga agaataaggc aatcaaaatg
     6721 acgcagaaaa ttgaacaatc tcaacgacaa gaacgggtag cggcctggaa tcgtcgcact
     6781 gaatgcgatc ttgccgcttt ccagaactca ccaaagcaaa cctaccaggc tgaaaaagcg
     6841 cgcgatcgca aactgtgcgc caacctggaa gaagcgattc gtcgctcagg tttacaggac
     6901 ggcatgacgg tttccttcca tcacgctttc cgtggcggtg acctgaccgt caatatggtg
     6961 atggacgtca tcgcgaagat gggctttaaa aacctgaccc tggcgtccag ctccctgagt
     7021 gattgccatg cgccgctggt agaacacatt cgccagggcg tggttacccg catttatacc
     7081 tctggcctac gcggtccact ggcggaagag atctcccgtg gtctgctggc ggaaccggtg
     7141 cagatccact ctcacggcgg tcgtgtgcat ctggtacaga gcggcgaact gaatatcgac
     7201 gtggctttcc tcggcgtccc gtcctgtgat gaattcggta atgccaacgg ctacaccggt
     7261 aaagcctgct gcggctccct cggctatgca atggttgatg ccgacaacgc aaaacaggtc
     7321 gtgatgctta ccgaagaact gctgccttat ccgcataatc cggcaagcat tgagcaagat
     7381 caggttgatt tgatcgtcaa agttgatcgc gttggcgatg ctgcaaaaat cggcgctggt
     7441 actacccgta tgaccactaa cccgcgcgaa ctgcttattg cccgtagcgc tgcggatgtg
     7501 attgtcaact ctggctactt caaagaaggt ttctccatgc aaaccggcac cggcggcgct
     7561 tcgctggcgg taacccgttt cctggaagac aaaatgcgta gccgcgatat tcgcgccgac
     7621 ttcgccctcg gcggtattac cgcgacgatg gttgatctgc acgaaaaagg tctgatccgc
     7681 aaactgctgg atgtgcagag ctttgacagc catgctgcgc aatcgctggc ccgtaacccc
     7741 aatcacatcg aaatcagcgc caaccagtac gctaactggg gttcgaaagg cgcatcggtt
     7801 gatcgtctcg acgtggtggt actgagcgcg ctggaaattg acaggcagtt caacgttaac
     7861 gtgctgaccg gttctgacgg cgtactgcgt ggcgcttccg gtggtcactg cgataccgcg
     7921 attgcctctg cgctttccat catcgtcgcg ccactggtac gcggtcgtat tccgactctg
     7981 gtggataacg tgctgacctg catcaccccg ggctccagtg tcgatattct ggtcacagac
     8041 cacggtatcg cagttaaccc ggcacgtccg gaactagcag aacgtctgca ggaagcgggc
     8101 attaaagtgg tttccattga gtggctgcgc gaacgtgcgc gtctgctgac cggtgaacca
     8161 cagccgattg aattcaccga ccgcgtcgtt gccgttgtgc gttaccgcga tggctcggtg
     8221 atcgatgttg tgcatcaggt gaaggaataa gccatgcacc tgcttcctga actcgccagc
     8281 caccatgcgg tgtcaattcc cgagctgctc gtcagccggg atgaaaggca agcacggcaa
     8341 cacgcctggc tcaagcacca tcctgttcca ctggtctcct ttaccgtggt tgcgcctggg
     8401 ccgattaaag acagcgaggt cacacgccga atttttaatc atggcgtgac agccttgcgt
     8461 gccttagcca caaagcaggg ctggcaaatt caggagcagg ctgcactggt ttccgccagc
     8521 gggccggagg gcatgttgag cattgccgcc ccggctcgcg atctcaagct cgccaccatt
     8581 gagcttgaac atagtcatcc tctcgggcgg ttatgggata tcgatgtcct gacgcccgaa
     8641 ggcgaaattc tctcccgccg cgactattca ctgccgcctc gccgctgcct gttgtgcgag
     8701 caaagcgcag ccgtctgcgc gcgtggaaaa acccatcaac tgaccgattt actcaaccgc
     8761 atggaggcac tgctgaacga tgtcgatgcc tgcaacgtca actaaaacca caaagcttgc
     8821 gacgtcatta atcgatgagt acgccctgct gggctggcgc gccatgctga ctgaagtcaa
     8881 tctgtcaccg aaaccaggcc tcgtggatcg cattaactgc ggtgcgcaca aagatatggc
     8941 gctggaagat ttccaccgca gcgcgctggc gattcagggc tggttacccc gtttcattga
     9001 atttggtgcc tgtagtgcag aaatggcacc ggaagcggta ctccacggat tacgcccaat
     9061 tggtatggct tgcgaaggtg atatgttccg cgccactgcg ggcgtaaaca cgcataaagg
     9121 cagcattttt tctttagggc tgctatgtgc ggcaattggc cgtttgcttc agctcaacca
     9181 gccggttacg ccaacaaccg tttgttctac ggcggcaagt ttctgccgtg gcctgaccga
     9241 tcgcgaactg cgtaccaata attcacgact gacggcaggt caacggttgt accagcagct
     9301 tggccttacc ggcgcacgcg gtgaagccga agcgggttat ccactggtga tcaaccacgc
     9361 cttgccgcat tacctcactc tgctggatca ggggttagat cctgaactgg cattgctcga
     9421 taccttgctc ctactgatgg ctaccaacgg cgataccaac gttgcatcgc gcggtggcga
     9481 ggggggcctg cgctggctac agcgcgaggc gcaaacatta ttgcaaaaag ggggcattcg
     9541 aacccccgcc gatctcgatt atctccggca gttcgacagg gagtgtatcg aacgaaatct
     9601 cagtccaggc ggcagtgccg acctgctgat ccttacctgg tttttagcac agatttaatt
     9661 atttaagcac ttgataaatt tggaaatatt aattttcgga gaacccgtat gtctttagca
     9721 aaagataata tatggaaact attggcccca ctggtggtga tgggtgtcat gtttcttatc
     9781 cctgtccccg acggtatgcc gccgcaggca tggcattact tcgctgtgtt tgtggcaatg
     9841 attgtcggca tgatcctcga gccaattccg gcaacagcga tcagttttat tgcggttact
     9901 atttgcgtta ttggcagtaa ttacctgctc tttgatgcca aagaattagc tgacccagcg
     9961 tttaatgcgc aaaaacaggc gctgaaatgg ggcctggctg gtttttccag caccacggta
    10021 tggctggtat ttggcgcatt tatttttgca ttagggtatg aagtttccgg gttaggtcgt
    10081 cgcattgccc ttttcctggt gaaattcatg gacaaacgca cgctgacgtt gggttatgcg
    10141 attgtcatta tcgacattct gctggcaccg tttacaccgt ccaacaccgc gcgtaccggg
    10201 ggtacggttt ttccggtcat taaaaacctg ccgccgttgt ttaaatcatt cccgaacgat
    10261 ccgtccgcgc gtcgtattgg cggctatttg atgtggatga tggtcattag taccagtctg
    10321 agttcatcca tgtttgtcac cggtgcggca ccaaacatgc tgggtctgga gttcgtcagc
    10381 aaaattgccg gtatccagat tagctggttg cagtggttcc tctgcttcct gccggttggg
    10441 gttatcttgc ttatcattgc gccgtggctt tcctacgtgc tgtacaaacc ggaaatcaca
    10501 cacagtgaag aagtggcaac ctgggcgggt gatgaactaa aaaccatggg tgcgctgaca
    10561 cgcagagagt ggacgctgat tggccttgta ttgctcagct taggtttgtg ggtatttggc
    10621 agtgaagtca ttaatgctac tgcggttggt ctgctggcag tttcgctaat gctggctctg
    10681 cacgttgtgc cgtggaaaga cattacccgc tataacagcg catggaacac gctggtcaac
    10741 ctggcaactc tggttgtgat ggctaacggc ctgactcgtt ctggttttat tgactggttc
    10801 gccggtacca tgagtacgca cctggaagga ttctcaccaa acgcaacggt gattgtactg
    10861 gttctggtgt tctactttgc acactacctg tttgccagcc tgtctgcgca caccgcaacc
    10921 atgctgccgg ttattctggc cgtcggtaaa ggtattccgg gcgtaccaat ggaacaactg
    10981 tgtatcctgc tggtgctgtc tatcggtatc atgggctgtc tgacgccgta tgcaaccggt
    11041 cctggggtga ttatttacgg ctgtggctat gtgaaatcaa aagattactg gcgtcttggc
    11101 gcaatcttcg gggtgattta catctctatg ttgctgttgg ttggctggcc gattctcgcc
    11161 atgtggaact aatacgttcg ccaaacgctg aataaatcat cacgcccacc aggtaagccc
    11221 acctggcggg cttttttatg atttaataga tagtattcag tgtatgtatg agttccacac
    11281 ccattatgaa agcattctgc cgtaacgccg cgttgctcgc ggtttctctg cttcccttat
    11341 cttctgccaa cgccgtagcg ttgcaggcaa aacagtatgg cgattttgat cgctatgtcc
    11401 tggccctctc ctggcaaacc ggattttgcc agagtcaaca cgatcgaaat cgtaacgaac
    11461 gagatgaatg tcgcctgcaa accgaaacga ccaacaaagc tgattttctg accgtacatg
    11521 gtctgtggcc aggattgcct aaatcggttg ctgcccgtgg tgttgatgaa cgccgctgga
    11581 tgcgcttcgg ttgcgctact cgcccaatcc cgaatctacc agaagcacgc gccagccgaa
    11641 tgtgttcatc gccggaaacc gggttatcac tggaaacggc cgctaaacta agtgaagtca
    11701 tgccaggagc tggcggacgt tcctgcctgg aacgctacga atatgccaaa cacggtgcct
    11761 gctttggttt tgatccggac gcatacttcg gtacgatggt acgcctgaat caagaaatta
    11821 aagagagcga agccggaaaa ttccttgcgg ataattacgg taaaacagtg agccgccgtg
    11881 actttgacgc cgcctttgcc aaaagctggg gaaaagagaa cgtgaaagca gttaagctaa
    11941 cgtgccaggg taaccctgcg tatttgactg aaattcagat ctcgatcaaa gctgacgcca
    12001 tcaacgctcc gctttctgca aactcattct tgccacaacc tcacccaggt aactgtggca
    12061 aaacctttgt gattgataaa gcgggttatt aatcctctga tttacaaaac acgctaattc
    12121 gtcttccata accatgccga ttaagaatta tacataatgc gctgcattga ggaattccct
    12181 gaatgtgacg caaatcactt caaagagtga gcaacagtca gtatcatcat gtgagttaaa
    12241 ccctcgccgc ctgacggtga gggttttctt ttgggtatgt ttatctgcga cactcgcagt
    12301 accgacgaca tggagtaaaa atgtccagac caactatcat cattaacgac ctggatgccg
    12361 aacgcatcga tattctgctg gagcaacccg cctatgctgg tttgccaatc gccgacgcgt
    12421 taaacgcaga gttggatcgc gcccaaatgt gttcgccaga agagatgcca cacgacgtgg
    12481 tgacaatgaa cagccgggtt aaattccgca atcttagaga tggcgaagtg cgtgtgcgca
    12541 cgctggtgta tccggcaaaa atgaccgata gcaatactca gctttccgtt atggctccgg
    12601 taggtgccgc actgctgggg ctgcgcgttg gcgattccat tcactgggaa cttccgggcg
    12661 gcgttgcaac ccaccttgaa gtgctggaac tcgaatacca gccagaagct gctggcgact
    12721 acctgcttta atcccatctg cacaagcgat aactcgcttt gcccagagga tgcactcgca
    12781 tcctctttac gcaaaatcag caatttttcc tggttctcac tgttcacttt tgctgcaaaa
    12841 ttattccgtt tcgtttgtgg gaataaccat ggcagaagca ttttacatcc tgataggatt
    12901 tctgattatg gcggcgatta tcgtcatggc tgtcctttac ctcgaaaacc actcctgacg
    12961 atcatattgt tccccccggc atcgcattca ccagacctga aaccgccttc tgcgccgcct
    13021 ctgcgctttg tgcaccgggt accagaatca ccttacggca ctcctcttca cgtttttcga
    13081 aaatctcata tccccgggcg gcctcttcaa acggcatata gtgggtaaca atttcttctg
    13141 gtttcagtaa tcctttctca attaacggta ataattctcc cagccatgcg tgaacgtggg
    13201 tctgtcccat tttaaacgtc aaccctttat caaaggcgtc gccaaacagg aaaccgtgaa
    13261 taaatccagc gtagacgccc ggtacgctaa caatgccgcc acgcctgacc gccgcaatac
    13321 actggcgcaa cgctttaccg ctgctgccct ccagtttcag gttagtcagc accgtttccg
    13381 tggtgctgcc tttcgcttca aaaccgacgg cgtctattac tgcatccacg ccccggtgac
    13441 ctgccgtttg ttcaataatt gactgtgccg gatcgctatc ttcatcgaaa ttaatcggga
    13501 tcgcgccgta gcggtcagcg gcgaaacgca agcggtaggg atgatgatca acaacaaaaa
    13561 tctgttccgc accgagcaac cgtgcacagg cgattgtcaa caatcccaca ggaccagcac
    13621 catagactgc aacgcttgaa ccttgttgga tctgcacatt ttttgctgcc tgccatgccg
    13681 ttggcagaat atcagaaagg aaaagggctt tatcatctga aagcaaaggc ggtactttaa
    13741 acggccccac attcccttta gggacgcgga catattccgc ctgcccgcca ggaacgccac
    13801 catacaggtg actataacca aacaatgccg cgggagcagg tatctgtttt ttattgagcg
    13861 cagcgccttt acccgcattg gtattttcgc aggcggcata ttgctgtaat cgacagaaaa
    13921 aacagtcgcc acaagcaatg acgaacggaa ttaccacccg gtcgcctttt tgcaaatttt
    13981 ttacgtcctt cccggtttca acgacttccc cataaattca tgaccaaaaa tatcgccatg
    14041 tttaacctgg ggtattttgc ctcgataaag atggaggtca gagccacaga tcgccgttac
    14101 cgtaatacgc agaataatat catctgcctg ttcaatgccc ggatcgggaa cattttctac
    14161 ctgaacgtga tgtgggccgt gataagtcaa tgctttcatg caacctccag agacagttca
    14221 aaaaagagtc agtctttaag ggtagctatc acagcgggaa aacgggcggt accgcaggtt
    14281 ttgggagaat tcctggtgtt cagtgagaat atattaatca cagaaaccgt tcgtaaggaa
    14341 aaagaaccag acaaatatca gggataaata ttataatcat ggcgaatgat ttgttcatga
    14401 ttaacaggga gaaagggtta tgtataaggc aatcattatg ccagttgatg tatttgaaat
    14461 ggaattgagc gacaaagctg ttcgccacgc tgaattcctc gcccaggatg acggagttat
    14521 tcatctactt cacgtactac ccgggtcagc cagcctgagc ctgcaccgtt ttgccgctga
    14581 tgtgcgtcgt tttgaagagc atctgcaaca tgaagcagaa gaacgtctgc aaacgatggt
    14641 cagccacttc accatcgatc cttcccgcat taaacaacat gtccgttttg gtagcgtgcg
    14701 ggatgaagtc aatgagttgg cagaagaact gggggctgat gttgtagtta tcggttctcg
    14761 caacccatcg atttcgaccc atctgttagg ttctaacgcc tcgagcgtga tccgccacgc
    14821 caatctgcct gtactggtcg ttcgctaaca gataaaagta tttatgccgc cctgcctgga
    14881 acatcccggc agggcttttt aatgccataa aaaagccgcc aggtttgacc ccggcggcta
    14941 agcaattgca ggtgaatctt acttcttctt atgcagtttt ggtgcgaatc aggtagtcaa
    15001 aagcactcag agaggctttg gcaccttcgc cagtggcgat gatgatctgc ttgtacggaa
    15061 ccgtcgtaca gtcacccgct gcgaacacgc ctttgacgtt ggtttcacat ttcgcatcaa
    15121 tgataatctc gcccatgcgg ttacgttcga ctgcgccttc cagccagttg gtgttcggga
    15181 gcagaccaat ctggacaaaa ataccggcca gttcgatgtt gtgaatattg ccgctgacac
    15241 gatctcgata ttccagacca acgactttgc tgccgtcgcc tttcacttcc gtggtttgcg
    15301 cattcagaat aatgtcgacg tttttcaggc tgcgcagttt gtcctgcaga acctggtcgg
    15361 ctttcatttc tggcgcaaat tccagcagcg ttacgtgctc aacgataccc gccaggtcga
    15421 ttgccgcttc cacgccggag ttaccgccgc cgataaccgc tacgcgtttg cctttaaaca
    15481 gcgggccgtc gcagtgcggg cagtaggtca cgcctttagt gcgatactga tcttcgcccg
    15541 gaacgttcat gttgcgccat tttgcacccg tcgccacgat aatgctgcgt gctttcagta
    15601 ccgcgccaga agctgtttca atctggtgta gaccaccttc aaccgctgcc gggatcagtt
    15661 tgctggcgct ctggctgtcg atcacatcaa cgtcgtattc atcaacgtga actttcagtg
    15721 cgcctgccaa cttctgccct tcagtcttcg gtacagaaat gtagttttcg atatcaacgg
    15781 tatcgaggat ctgaccacca aaacgttcgc ccatcagacc ggtacggatg cctttacgtg
    15841 cggagtaaat tgctgccgct gcacccgccg ggccggaacc aacgattaat acgtcataag
    15901 catcacgctt gttcagctct tctgccgcac gtttttccgc gccagtatca attttggcaa
    15961 cgatttcagt caacgtcatg cggccctgac caaactcttt cccgtttacg aacactgccg
    16021 gaacgcccat cacgttgcga tcggtgattt cgttctggaa ggtgccgccg tcaattgcag
    16081 tgtgcttgat gcgcgggttc aatacgctca tcaggttcag cgcctgcacc acgtccgggc
    16141 agttatggca agagagcgag taataggttt cgaattcaaa atcaccgtca atatggcgaa
    16201 tctgctccag cagagactgc gcttctttcg acggatgacc accggtccac agcaacgcca
    16261 gtaccagcga ggtgaactcg tggcccagcg gggagcctgc aaaacgtggt ccctggttgg
    16321 aacctgggtt ggtgatcagg aaagacggct tacgcactgg caagctgtta tcttctttaa
    16381 aggtgacttt gtctgacagt tctgcgattt cagccaacag ttccttgatt tctgccgatt
    16441 tagcgctgtc atccagcgtg gcaattaact caacaggctt ggtcaatttc tcaaggtaag
    16501 ccttgagttg agttttcata tttgtgtcga gcatgtttat ctcctgggct ttaaacatca
    16561 tcatgcaagc ggcctctgcg gctacctgga tgcagcttgc atcatggtgc ggggaagaaa
    16621 atcgggtgcc gcagcacccg aagaattagg tgaatttccg aagtatttcg cgtcagagca
    16681 aggcgactag cctgtgaatc cccgggagct tacacaagta agtgaccggg gtgagcgggc
    16741 gacgccaacg ccgctatggc gtgaaagacg aaggaaattt agattttacc aaccaggtcc
    16801 agagacggag ccagagttgc ttcaccttct ttccatttag ccgggcaaac ttcacctggg
    16861 tgagaagcta cgtactgagc tgctttgatt ttacgcagca gatcagacgc gtcacggcca
    16921 atgccttcag cggtaacttc gattgcctgg atgataccct gcgggtcaac aacgaaggtc
    16981 gcacggtcag ccagaccttc atcttcacgc atgttgtcga agttacgggt cagggcgcca
    17041 gtcgggtcgc cgatcatcgc atatttgatt ttagcgatgg tttcagagct gctgtgccat
    17101 gctttgtggg tgaagtgagt atcggtagat actgcgtata cgtctacgcc cagtttctgc
    17161 agttcttcgt agtggtcagc aacgtcaccc agttcggtcg ggcatacgaa agtaaagtca
    17221 gccgggtaga agaagaagac gctccagcgg ccttcggtat ctttttcggt gatttcgatg
    17281 aattcgccgt ttttgaatgc ctggttttta aaaggtttaa ttttggtgtt aatcaaggac
    17341 atctatactt cctccgtgtt ttcgatgaga tgtaaggtaa ccaatttttg ccgattcggc
    17401 taatgcgttt ccattatcaa atcgataagt tttaccttac aaccttcgta agacaacttt
    17461 gtgaactttg caccgccagc ggcgataaaa agtaaaatgg ccccctttcc aggtgaccgt
    17521 gtaatctgaa ctaccctttg gtatcttcag cgcctgcctg caggctgagg tgttgccctt
    17581 acaaatgcaa caacgacatg gattacaaca ccctcataaa caaagggcaa tcacctgatc
    17641 taagctctta cctatgacag tgataggtta tgccttttac tcgacttttg cactgactga
    17701 aaagaacaaa ttaatgttaa aaaagatact tttactggct ctgcttcctg caatcgcctt
    17761 cgcagaggaa cttccttccc cagtaaaagc gattgaaaaa cagggcatta caatcatcaa
    17821 aacgttcgat gcccccggag gaatgaaagg ttatctcgga aagtatcagg atatgggcgt
    17881 caccatctac ctgactcccg atggtaagca cgctatctct ggttacatgt acaacgagaa
    17941 aggtgaaaac ctgagtaaca cacttatcga aaaagaaatt tacgcaccag ccggacgcga
    18001 aatgtggcaa cggatggaac aatcccactg gctcctcgac ggtaaaaaag atgcgccggt
    18061 cattgtctac gtcttcgccg atccgttctg cccatattgt aaacagttct ggcagcaggc
    18121 gcgcccgtgg gtagattctg gcaaagtgca attaagaaca ttgttggttg gggttatcaa
    18181 gccagaaagc ccggcgacag cagcggcaat tcttgcctcc aaagatcccg caaaaacctg
    18241 gcaacaatat gaagcctctg gttgcaagct taagctaaac gtgcctgcaa acgtaagtac
    18301 agagcaaatg aaagtgttaa gtgacaatga gaaactgatg gacgatctgg gggcaaatgt
    18361 cacgccggct atctattaca tgagtaagga aaatacgctg caacaggccg tggggttgcc
    18421 cgatcagaaa acgcttaata tcattatggg aaataaataa aaggtaattc atggatggtt
    18481 gaactatatc cctgcttttc aaccatccta aaaaagcatg aattgctcgc tctaccctaa
    18541 gtggctgtgc tattttagaa aaacttaatc ctgagttaag caaaaaaaca taatcaataa
    18601 aaatacatac gcaaaaaaca tttattaagt gaatatacca tggaagaaaa atataaaccg
    18661 gagtagtgta tggccaatct ctacgacttg aaaaagttcg acttaaatct tcttgtcata
    18721 tttgagtgta tttaccagca tttaagcatt agcaaagctg ctgaatcact atatatcacc
    18781 ccatcagccg tgagccagtc gttacaacgc ttacgtgcgc aatttaatga ccctttattc
    18841 attcgctcag gaaaaggtat cgcgccgacg acaacagggt tgaatctgca tcaccatctt
    18901 gagaaaaacc tcaaaggcct tgaacaaaca attaatatag tcaataagtc cgaactaaag
    18961 aaaaacttta ttatctacgg ccctcagctc atttcttgct ctaataatag tatgctgatc
    19021 cgctgcctac ggcaagatgc ctctgtcgag attgagtgcc atgatatcct catgtcagca
    19081 gagaatgctg aagaactgtt ggttcaccgc aaagccgatc tggtgatcac ccaaatgcca
    19141 gtgatcagcc gttccgtcat ttgcatgcca ttgcatacaa ttcgcaatac tctaatttgt
    19201 agtaataagc atccgcgaat tactgacaat agcacttatg aacaaattat ggcagaagag
    19261 ttcacccaat taatatccaa atcagcagga gttgatgata ttcaaatgga aatagacgag
    19321 aagtttatga atcgcaaaat ttcatttcgt ggttcctctt tgctgacaat aattaatagc
    19381 attgcagtta cagatttact gggaatagtc ccctatgaac tttataacta ccaccgtgac
    19441 tttcttaatt taaaagagat aaaactagaa catccgttac cttcaataaa gctttacatt
    19501 tcctataata aatcttcttt aaataatttg gtgttctccc gatttattga ccgtctaaat
    19561 gagagttttt gatatataac tacagacagc tactataact ttcatctatt tattcacagc
    19621 tcgaatgaaa aataaaacac tctgctttac agtgtttact ttataattaa cagtcttatc
    19681 tcactgttca ccctgtaacg ttcagagagc gtcttcagat gtctatttat aaaattcctc
    19741 ttccactcaa tattctggaa gctgcacgag agcgtattac ctagacactc aacaccctac
    19801 cccgcgtttg tgtctcattt tctggcggca aagactctgg tttaatgctg catctgacag
    19861 ccgaacttgc ccgacaaatg ggcaaaaaaa tctgcgtttt gtttatcgac tgggaggc
//
LOCUS       DAETPZ010000071        19906 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.071, whole genome shotgun sequence.
ACCESSION   DAETPZ010000071 DAETPZ010000000
VERSION     DAETPZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.071"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..375
                     /locus_tag="K0W59_002864"
     CDS             <1..375
                     /locus_tag="K0W59_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HBI3023825.1"
                     /translation="GAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMS
                     ERPIWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPIRCAFTGALTLYYYFIG
                     FFGILAAIAITLVWQRHTRFFH"
     gene            complement(411..1367)
                     /locus_tag="K0W59_002865"
     CDS             complement(411..1367)
                     /locus_tag="K0W59_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HBI3023826.1"
                     /translation="MSKSHPRWRLAKKLLTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IITALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1367..2608)
                     /gene="clsB"
                     /locus_tag="K0W59_002866"
     CDS             complement(1367..2608)
                     /gene="clsB"
                     /locus_tag="K0W59_002866"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HBI3023827.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQCEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2605..3366)
                     /locus_tag="K0W59_002867"
     CDS             complement(2605..3366)
                     /locus_tag="K0W59_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBI3023828.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3499..3909
                     /locus_tag="K0W59_002868"
     CDS             3499..3909
                     /locus_tag="K0W59_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HBI3023829.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(3871..4977)
                     /locus_tag="K0W59_002869"
     CDS             complement(3871..4977)
                     /locus_tag="K0W59_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3023830.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(4988..6121)
                     /locus_tag="K0W59_002870"
     CDS             complement(4988..6121)
                     /locus_tag="K0W59_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3023831.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6114..7850)
                     /locus_tag="K0W59_002871"
     CDS             complement(6114..7850)
                     /locus_tag="K0W59_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI3023832.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(7843..8838)
                     /gene="hlyD"
                     /locus_tag="K0W59_002872"
     CDS             complement(7843..8838)
                     /gene="hlyD"
                     /locus_tag="K0W59_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HBI3023833.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(8841..9512)
                     /gene="cecR"
                     /locus_tag="K0W59_002873"
     CDS             complement(8841..9512)
                     /gene="cecR"
                     /locus_tag="K0W59_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HBI3023834.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEETERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHSHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9741..10928
                     /gene="rhlE"
                     /locus_tag="K0W59_002874"
                     /pseudo
     CDS             9741..10928
                     /gene="rhlE"
                     /locus_tag="K0W59_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(11135..11617)
                     /gene="ybiA"
                     /locus_tag="K0W59_002875"
                     /pseudo
     CDS             complement(11135..11617)
                     /gene="ybiA"
                     /locus_tag="K0W59_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
     gene            11737..13887
                     /gene="dinG"
                     /locus_tag="K0W59_002876"
     CDS             11737..13887
                     /gene="dinG"
                     /locus_tag="K0W59_002876"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HBI3023835.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            13915..14883
                     /gene="ybiB"
                     /locus_tag="K0W59_002877"
     CDS             13915..14883
                     /gene="ybiB"
                     /locus_tag="K0W59_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HBI3023836.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPL
                     LAILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPV
                     GAFCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFS
                     DIGGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETT
                     AQWTERCLAGSEAIPESLKIQMACCLVATGEVATISDGLARVNQAF"
     gene            15024..16109
                     /gene="hcxB"
                     /locus_tag="K0W59_002878"
     CDS             15024..16109
                     /gene="hcxB"
                     /locus_tag="K0W59_002878"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HBI3023837.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(16338..16598)
                     /locus_tag="K0W59_002879"
     CDS             complement(16338..16598)
                     /locus_tag="K0W59_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBI3023838.1"
                     /translation="MKTINTVVAAMALSTLSFAVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(16890..17129)
                     /locus_tag="K0W59_002880"
     CDS             complement(16890..17129)
                     /locus_tag="K0W59_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DksA/TraR family C4-type zinc finger protein"
                     /protein_id="HBI3023839.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNR"
     gene            complement(17203..17880)
                     /gene="ybiX"
                     /locus_tag="K0W59_002881"
     CDS             complement(17203..17880)
                     /gene="ybiX"
                     /locus_tag="K0W59_002881"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HBI3023840.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(17922..19906)
                     /locus_tag="K0W59_002882"
                     /pseudo
     CDS             complement(17922..19906)
                     /locus_tag="K0W59_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         4618 a         5226 c         5379 g         4683 t
ORIGIN      
        1 ggcgcgatgt ttttaatcag tatgctggcc ggtttattat ttaatgttga tcctggttct
       61 caccgtttta tcattatgat gacgttgaca gggttggccc tggtaatcat cgtgaatgcg
      121 gcattaatga gtgaacggcc catttgggta ataagttgct taatgattgt gttatggcca
      181 ggcattatct cgcatggacg aaataagctc cttgaactgg caggaaaatg ccatagtgaa
      241 gagttgtgga gtccgattcg ttgcgctttt acaggtgcat taacactcta ttactatttt
      301 atcggcttct ttgggatact tgccgcgata gctataacgc ttgtctggca aaggcatacg
      361 cgtttttttc attagtcgta aatgcagata ctaaagaact taactaaact tcacatcgcc
      421 gcttcatttt tcgcccgtag cttcttcgct tggctttcca gtagcaggta acagatcagc
      481 gccagcagca gcgggataaa gtaatacagg acgcgataag cgagcagggc ggtgataatg
      541 ctccctttcg aggtatgctc cccagccagt agtgcgataa acaccgcttc cagcacaccg
      601 atccccgccg gaatatgcac gatgacgcca gcaatactac taaccagtaa cacgcccagt
      661 acaaagaaat agttcacgct ttgaccaagt aacagccaga taatcgcccc cattaccatc
      721 cagttaacgc tggaaatcag catctgagcg agggcgaatt tccatgaagg cagcaccagc
      781 ttttgccctt tgatggtcat atggcggtgc ttcgcgaaag cgcaaaacca caaataaacc
      841 gcgataatca tcagtaagcc aatgccgaga atacgcagcg tagtttgatc gacataccag
      901 tgatccggca actccaccac gcctgcggta aagataatcc ctgccagtaa aatatagccc
      961 agccagttgg tggtaatact gagcgaggaa atccgcgtaa tagtgctgcc cggtaaccct
     1021 agtcgggagt acaaacgata gcgcatacca atgccgccga cccaggtact gagcgtcagg
     1081 ttgaaggcgt agcagataaa cgacaccagc atcacctggc gcttcgccag tttgtgaccg
     1141 cagtaaaagc gggcgagcag gtcatagcag ccgtaaatca gatagctgac gaccaccagc
     1201 ccgaccgcac taagcagcgc aacgcgattg tagtcgcgga tgaccttcca gacctcttcc
     1261 cagtccactt ttttggcgta gaccaccagc aacacaatca ccgcgataaa aaacagccag
     1321 gtgaggagct tctttgctaa gcgccagcgc gggtgtgatt tactcatcag ggttttaccc
     1381 ccgtgttttc agtttctacc cgatcctgcg tttccattgt cggttgtgcg gggggatcaa
     1441 cctgcgtcag acgtggtgtg tgtgccggaa gccagccaac cagcgccggg aagtggcgta
     1501 aaaagtggaa cgccagcacg cttttggtca ggttccacca ggtgcgtttg ggcagcatgg
     1561 tttcatccac ctgctgacaa tctgcggcaa taatgccgtt cagattatcg cgcagcgtct
     1621 ggttaaaatg acgatcgtgg atgatgacat ttgcttcgag attcagtgac aaactgagcg
     1681 gatcgagatt actggaccct actgtcgccc agtgatcgtc catcaatgcc actttgccgt
     1741 ggagcgggcg gcggcggtac tcaaaaacct gaacgccgcc tttaaccaga tagttataca
     1801 gcaagcgcgc accgactctg acaatcggca tatccggttc gcactgaatg atcagtttga
     1861 tccgcacccc gcgccgtgcc gctttacgca aggcgtgtaa aaatcgatag ccggggaaga
     1921 agtaggcgtt ggcgataatc acttcccgcc gcgcctgagt gagcattttc agataatggc
     1981 gttcaatatc atcgcgatgt tcttcgttat cgcgccagac cagcaatacc tgcgcttctc
     2041 ccggctggcg gttctcttcc gctttgtgat gacgtcgcca ccagcgtcgt gccgcgctct
     2101 gtccaggcag gttttccagc tcaaactgga gaatatcttc gacaatcggc ccttcaaggc
     2161 gtaccgcgta atcctgttta gcctctggac cgtagctgga catatgctcg gcggagtaat
     2221 tcagcccgcc aataaaggcg atacgcgcgt cgatcaccac aattttgcga tgcatccggc
     2281 gaaacacatt ggtgcgcata ccaaaaaggc gagggcgggg atcgtagtag cggaacacta
     2341 cgccagctgc cgtcagttca ttgacaaact catcgctgag atccggcgaa ccgtagccat
     2401 ccagcaagac ttccgcttta accccgcgtt gcgctgctgc cagtagtgcc gcatgcagct
     2461 gtttgccgac gttatcctca aaccagataa acgtttcaag aatgatgcgt tcttgtgcct
     2521 cgccaatcgc cttaaacacc gcgggataat attgctcgcc gttttccagc aactggatct
     2581 tattgccttc gcgccagcta catttcataa atgaatctcc gcacttaaag gggcatgatc
     2641 agaaaggtgt cgccatgtcc gcagcggcaa cgcggttggc gcgctggcgc tggcattttt
     2701 gacgtagatc ctgtccagtc gtagtagagg aaattgcacc ggaaacgtgc gcgccgggcg
     2761 tccgtgggcg cgggtaaaaa tctcatccag tccggcctgc acttttaacg gatgattagc
     2821 tttttgccgc cagtcattga aatcacccgc caccaatacc ggttcgccgt ccggtagctc
     2881 attcacccat tcggcgagca tcgcaagctg cgcctgacgg tgcgcctcac gcaggcccag
     2941 atgtacgcac atcacatgaa tcgcttttcc ggtcatcggc ggcacaatgc ggcagtagag
     3001 cacgccgcgc ttttccgcac catcgaccga aacatcgcga ttctcataat gttcaatggg
     3061 ataacgcgac agtacggcgt tgccgtgatg cccttccggg tatacggcat tgcgaccgta
     3121 ggcaaaatcg ctccacatag tgtcggcgag aaactcgtag tgcgaggtat cgggccagtt
     3181 ttccacatgc agcggatgaa cttcgtgcgc gcccatcact tcctgcaggc aaacaatatc
     3241 ggcgctgacg gtacgcacgg catcgcgaag ttccggcaaa atgaagcgtc ggttaaacgc
     3301 ggtaaagcct ttgtgaatgt tgatggtgag caccttgaac gaaaattgtt gtgtttgatc
     3361 gggcataatt ttcctgtctt tgcctcttat ctcattgaaa tagtgtagtc ggcgtcacaa
     3421 aaaggtgcgg tcttacggaa ttttccgtaa agttcggtac tctgagtaag tagagataaa
     3481 ttcttcagga gagaagccat gaagtggcaa caacgtgttc gtgtcgcaac gggtctaagt
     3541 tgctggcaga ttatgttgca tttactggta gtggcgctgc tggtggtggg ctggatgagt
     3601 aagactctgg ttcacgtcgg cgtgggatta tgcgcactgt attgtgtcac ggtagtgatg
     3661 atgctggtgt ttcagcgcca ccccgagcaa cgctggcgtg aggtggcaga cgtgctggaa
     3721 gagctgacca cgacctggta ttttggcgca gcgctgattg tgctgtggct gttgtcccgc
     3781 gtactggaaa acaacttttt gctggcaatt gcagggctgg cgatccttgc tggcccggcg
     3841 gtagtgtctt tgctggcgaa agataagaag ttacatcacc ttacgtctaa acatcgcgta
     3901 cgccgctgac cctgtcgtgg ccgttatcac cagtagcggc cacaaactat tccacacaat
     3961 atccagactc gcatccttca aataaatctg cttggtaatg tccgtaaagt ggcgaatagg
     4021 gttaatccac gtcagatttt gcagccacac tggcatgttt tccaccggcg aaacgtaacc
     4081 ggaaaggaga atggcgggca tcataaaaac aaacacgccg ataaacgcct gctgttgtgt
     4141 cgagcagagt gatgaaatca acagaccgaa tcccaccagc gataaaccgt aaatcaccat
     4201 cgtaaagtag aacagcgcta gcgatccggc gaaggggatt tgatacgccc agataccaat
     4261 cgccagcaca atggtggcct ggaaggtggc gacaattaac gccggtacgg ctttgccgat
     4321 gaagatctgc caggtggtga gcggcgaaac cagtagctga tcgagcgtac cttgttcacg
     4381 ttcgcgggcg acggaaagtg aagtgacgat cattacgccg atagtggtga tcatggcgat
     4441 cagcgacggc accacaaacc atttgtagtc gagattcggg ttataccagt tgcgtaccac
     4501 cagctcgctg ttgttgggtt tcggttttcc ttccagcagc tcctgctgat aatttttgac
     4561 gatctgttgc aggtagttgg cggcaatttg cgcactgttg gagttacgcc cgtcgaggat
     4621 caactgcaac ggcgcggtct ggaaggtatc cagtttgcgc gagaagtcag ccgggaaacg
     4681 caccagtagt aacgcctttt gtgtgtcgat ggtcgggcgg atctcctgtg ggcttttcag
     4741 cagcagcaca tgtgtaaagg cgctggcgcg ggcaaaacgt tgagtaagtt ccaccgaatg
     4801 ctcgccgtta tcttcatcgt agatggcgat ggtggcgtta gtcacttcca gcgtggcggc
     4861 gaacgggaac aggatcacct gaattagcac gggtaaaatc agaatcgcgc gggtttgtgg
     4921 ttcgcgcagc aacgactgca actctttgcg gattaacgtc cataagcgat gaaacatgct
     4981 cttctcccta atccagccga cgtttggttt tcagccacgt caggccgata aacatcaccg
     5041 ccgaagcgat caaaaacagc acgtttacca ccagcaccac tggaatattc ccggcgagga
     5101 acaggctttg cagggtgctg acgaaataac gagcgggaat aatgtacgtc accgcgcgga
     5161 tcaccgcggg catactgtcg atctgaaaaa taaagccgga aagcataatc gacggcagaa
     5221 aagcggcgtt cagggcgacc tgagcggcat tgaactggtt gcgggtaatc gtggaaatca
     5281 gcagccccat tcccagggta ctgagtaaaa acaggctgga gataaaaaac agaatcagca
     5341 gcgaaccgcg atacggcacg ccgagaataa acactgacac cagcatacac agcaacatcg
     5401 ccagcatccc gagaaagtaa taagggatca gcttacacag cagcagttcc gtgcgggtaa
     5461 tctccgtaga gagcagagcc tccatggtgc cgcgttccca ttctcgcgcc accaccagcg
     5521 aggtgagaat cgcgccgatg accgtcatga taatggtcac cgcacccggg ataataaagt
     5581 gctggcttat cgccgccggg ttaaaccagt agcgggtttg tacatcaata agcggttcaa
     5641 aagtctgccc gttgtcctcc gctcgctgca tttgccagat ctgccagatc ccttcgacat
     5701 acccctgcac aaagttagcg gtattcggct cactgccgtc ggtgatcacc tgaatcggtg
     5761 cggtggcgtt ggcgcgctcc atctgttcgg cgaaatccac gggaataacc accagaccgc
     5821 gaattttccc cgactgcatt ttggcgatca gttcctgacg gttatcgctg atggtggcgt
     5881 cgatgtaggg cgaaccggtc atggtgtggg tgaaatccag cgccgcttcg ctacgctgtt
     5941 ccagcaaaat cccgacccgc agcttgctgg agtcgaggtt aatgccgtaa ccaaaaataa
     6001 acagcagtag cagtgggatc actaccgcaa tcagccagct actcggatcg cgaacgatct
     6061 gccgcgtctc tttgacgcac agcgcccgca cgcgatgcca ggaaaggatc gggttactca
     6121 ttgctatgct ccttatccca gtcgtggatc aactgaataa aggcttgctc catggtggga
     6181 tcgggttgct catcgttagc tgactgtgct ttcaaatcgt ccggcgtacc gctggcgatt
     6241 aatttcccac ggtacaccag gccgatgcgg tcgcaatatt ccgcttcatc cataaagtgg
     6301 gtggtgacca tcaccgtgac gcctttctct accatgctgt tgatgtgcag ccaaaattca
     6361 cggcgggtga gggggtcaac gccggaagtc ggttcgtcga gaaagagaat atccggttca
     6421 tgcatcagcg aacaggccag cgccagccgc tgtttaaaac ctaatggcag ttcatcggtg
     6481 gcgtgtgagg caatactttt caggccaaat gcctcgctca tacgggagat tttttcgttc
     6541 tgcgcccgac cgcgtaagcc atacacacca gagaaaaagc gtaaattctg ttcgaccgtc
     6601 aggttgccgt agagcgaaaa tttttgcgcc atatagccga gatgctggcg cgctttaccg
     6661 gaactctctt tcagatccat ccccagcacc agcgcctggc cggaagtcgg caccagcaaa
     6721 ccgcacatca tcttaaaggt ggtcgattta cccgcgccgt ttggccccag caaaccaaaa
     6781 atctccccac gtttaacggc aaagttgacg tgatcggtgg cggcaaaatc accaaatttc
     6841 ttggtcagtt ctttcgcttc gatcaccgtc tcgccgggtg tgccttctac cgtatgcaat
     6901 attgcaccca gcggcgattc tgaggttccg gcaccgccca gcaaatcaat aaacgcatct
     6961 tcaaaacgcg gcgtagtttc gttgatgttg atttccggca tcccgtcggc atggcgaata
     7021 tcgtctggtg tggcctcttt tttgaggatc agacgtaccg atttcccctg aatcatgccg
     7081 tcgctgacct gcggcagttt caaggcgcgt tgcaacagtt tgcggttgcc ctcgtgtgga
     7141 ctggtcatca gaaagctgcg tccggccatg gtttgtgtca gggcttttgg ttctccctga
     7201 tacagcaact cgccttcgtt catcagtaac acgtcacggc actgctcggc ttcgtcgaga
     7261 tacgaggtac tccagaggat taacatccct tcgcccgcca gctcatgcac catctgccac
     7321 agttcgcgcc gtgagatagg gtcaacgccg acgccgggtt catcgagcag caacactttc
     7381 ggttcgccca ccagggtaca ggccaggccg agtttttgtt tcatcccacc ggagagcttg
     7441 cccgccaggc gtccggtaaa cggcccaaga gacgtaaact ccagcaggcg agcaaaagtt
     7501 tgcttacgtg cctcgccggt gacgctgcgc aaatccgcgt acagattgag gttctccatc
     7561 accgtgagat cttcatacag accaaatttc tgcggcatat agccgagcac ggcgtgcagc
     7621 gcgccgtcgt ttttgatcgg atcaaagcca atcaccgtgg cactgccgct gtcgggtttc
     7681 agtaatcccg ccaacatccg catcagcgtg gttttacctg caccgtccgg ccccaccaac
     7741 cccgtcacat aaccggcgtg aatggtacaa tcgagcggcg cgacggcggg cttgtccatg
     7801 cccggaaagc gtttttccag gccgttcagc gtgataacgg catcattcat gtcctgcctc
     7861 gtcaccgaat tgtaccgtca ctggcattcc ctggcgtaac gcatcatcgg cgtcggtcac
     7921 cacaatacgc aggcgataga cgaggtcggt acgcagatcc ggtgtttcga cggttttcgg
     7981 ggtaaattca gcagtcggcg aaacgaaacc aatctgcccg tgatacggct tgtccgggcg
     8041 accatcggta taaagcagca ctttgcgccc cggctgggcc tggtcaagat tccgttcatc
     8101 aacataagcg cgcacccaca ccggacgcgt tagtgaaacg gtaaacaccg tgccaccttc
     8161 attgagaacc gtgcctggct ctaccgcgcg cgttaacagc gtgccatcag acggtgctat
     8221 caacgttgag tcctgtaaat tcagctccgc ctgcgccagt tgcgcctgcg cctgttcgag
     8281 gctggctttc gcctgagcga tatcctgttc acggttaccg gaacggtact gacgcaattt
     8341 atcctgtgct gatttcagcg ttgcctgtgc ctggtcgcgc gaggagcggg cattttccag
     8401 gtcatttgcc gaaatggtgc ggcttttcca cagtccttgc tggcggttat agaagttctg
     8461 cgcatagtca taggcggctt gcgcctgttt caccgctgcg gcggcctgag cgatttcttc
     8521 atcgcgatac ccggcaagca tcagatcata ctgcgcctgt gccaccgaga cgcccgcttt
     8581 cgcctgcatc agggcaatct catacggctt gtgatccagt tcgcccagca cctggcccgc
     8641 tttgatagca tcaccttcgt ccaccgccag cgattcaacg cgccccccaa cacggaaact
     8701 aagattcacc gtacgaatat ccacgttgcc atacagcgtc aggccgttat cctggcggct
     8761 ttgataccac cagtagcctc cggcaaccac ggcggcaagt accactaccg ccagtccgat
     8821 cacgacaggt tttttcatca ctacaaactc ctttgcgata atccttgcaa aatcaggtcg
     8881 atatgacagg tcaccgtctg gttgatcagt tcggtctttt cttcatcgaa cgcggtccag
     8941 ccggtacgta acagaattgt ttctttgcca agacggaacg ccagaatctc accaatcaac
     9001 gcatgggaat gaaggatcat gcgggtgtca ttagcgtcgc agccggtcca ggcggcaatc
     9061 aggcgtgtca ggtggctgtg tagcggacta atcacctgtt cgtgcaccag gtggtaggct
     9121 gccgtgggag agagctgctc acgggagata aacttgctga ggttgacggt gtcatcctgg
     9181 gtgagcagct taatcatgtt cctgcaggcg cgaaggatca gttcacggat ggcagcccga
     9241 tcaggctgtg gttgggcgaa caagcgttcg gtttcctcgg catgcggacg gaactgctcg
     9301 ccaataaaat cggcaatcca ctgggcgcag gcgaggtaca aatcttcttt cgaaccgaag
     9361 tagtaggtga tggcagcgat attctgcccg gcctgggcgg ctatctcgcg ggtggtggcg
     9421 ttcattccat attcaccaaa ctgcgccagt gcggcagcaa tcagctgttt tttcgcctgt
     9481 tcacccttga ttgtcatggc aggattattc atcgcacagt ccattcttaa tcaaatgatt
     9541 gattaagatt atgactccat atgggagttg tccagtatgg ctaagaattt tagctacgcc
     9601 agtcacaggg ataatttatg cgctgcgtca caaaaactgc tacactccgc tccctcatga
     9661 cattgtggtt tttgtcattt tccttttcag tatctccctg aaaactacac cggtaacggt
     9721 cggggcggtt cggagtagtt atgtctttcg attctttggg tttaagccct gatatcctgc
     9781 gcgccgttgc cgagcagggt taccgtgaac ccacccctat tcagcagcag gcgatccctg
     9841 cggtgctgga aggccgcgac ctgatggcta gcgcccagac cggcaccggc aaaacagcgg
     9901 gctttacgct gccgctgttg caacacctga tcactcgcca gccgcacgcc aaagggcgtc
     9961 gtccggtacg tgcgctcatt cttaccccga cccgtgaact ggcggcacag attggcgaaa
    10021 acgtccgtga ttacagcaaa tacctgaaca ttcgttcgct ggtggtgttt ggcggtgtca
    10081 gcattaaccc acaaatgatg aagttgcgcg gcggcgttga tgtgctggtg gcaacgccgg
    10141 gacgtttgct ggacctggaa catcaaaatg cagtgaagct ggatcaggtt gaaatcctcg
    10201 tcctcgatga agctgaccgc atgctcgaca tgggctttat ccacgatatc cgtcgcgtgt
    10261 taacaaaact gccagcgaaa cgccagaacc tgctgttctc tgcgaccttc tctgacgata
    10321 ttaaagccct ggcggaaaaa ctgttgcaca acccgctgga aatcgaagtg gcacgccgca
    10381 ataccgcgtc tgatcaggtg acccagcacg ttcactttgt cgataagaaa cgcaaacgcg
    10441 aattgctgtc gcacatgatt ggtaaaggga actggcagca ggtgctggtg tttacccgta
    10501 ccaaacacgg cgctaaccat ctggctgagc agctcaataa agatggcatc cgtagtgtgg
    10561 cgatccacgg gaataaatcg caaggtgcgc gtacccgtgc gctggctgat tttaaatcgg
    10621 gtgacattcg cgtactggtg gcgaccgata tcgctgcgcg cggtctggat atcgaagagt
    10681 tgccgcacgt cgtcaactat gaactgccaa acgtaccgga agattatgtc caccgtatcg
    10741 ggcgtaccgg tcgtgcggct gctaccggtg aagcgttgtc gctggtgtgt gttgatgaac
    10801 acaaactgct gcgtgatatc gaaaaactgc tgaaaaaaga gatcccgcgc attgcgattc
    10861 cgggctatga gccggacccg tcaatcaaag ccgaaccgat ccagaacggt cgccagcaac
    10921 gtggcggcat caggctgatg aacaaacgca aaactgcctg atgcgctacg cttatcaggc
    10981 ctacgtgaac tctgcaatat attgaatttg catgcttttg taggccggat aaggcgttta
    11041 cgccgcatcc ggcatttcac aacaagcact tgtcagcaat ttgagaacac gggaaaaaat
    11101 ttatctgttt taccgcccat aagccaccga gacgttactt ctctatagcc aattgttcgc
    11161 gcaactccat taaaaggtag cccagtcgat tcttgccctt accattacca ccgtctcccc
    11221 agtaagcatc gttttccgta tgctcaacca gttttgcggg cgcggttgcc agcaagagca
    11281 cacgcagttc tgcatgctgt tcgaatttag cgcgaagtgc tttacgcatc acctgttctt
    11341 taaccgactc ccagttttta cgaagtggct tggaacgatt acgccccatg cgagcggcga
    11401 ccatggggga agaaacccga cggatctctt cgcggtattt ttcatcaagg aatttttgtg
    11461 cctgaaaata gtgttcggag gtgggctagg tattcccgtc aaccttgatg ggcctggcgg
    11521 caaagttaga aaaatcaccg tagtcatcgc tggtgctgta gaaatttatg atggtgtctt
    11581 gcatgacgtg ttggatcctt tgtgctcgaa cgggcattaa accgcattat gttggtggtt
    11641 attgcgagcc gctttccaga aacagaaaaa ccattacccc tgaaaaccga aaaatgccac
    11701 aatattggct gtttatacag tatttcaggt tttctcatgg cattaaccgc cgcgcttaaa
    11761 gcgcaaattg ccgcctggta taaggcgctt caggaacaga tccccgactt tattccccgt
    11821 gcgccgcagc ggcagatgat tgcggacgtc gccaaaacgc tggccggaga agaagggcgg
    11881 catctggcga ttgaagcccc caccggcgtt gggaaaacgc tctcctattt gatccccggc
    11941 atcgccattg cccgcgaaga gcaaaaaacg ctggtggtga gtaccgccaa cgtggcgttg
    12001 caggatcaga tttacagcaa agatttaccg ctgctgaaaa agatcattcc cgatcttaaa
    12061 ttcactgccg ctttcgggcg tgggcgctac gtttgtccgc gtaatctgac ggcgctcgcc
    12121 agcactgaac ccacgcaaca ggatctgctg gcgtttcttg acgacgaact gacgccaaat
    12181 aatcaggaag agcaaaaacg ttgtgcgaag ctgaagggcg atctcgacac ttataaatgg
    12241 gatggtctgc gcgatcatac ggatatcgct atagatgatg atctctggcg tcgtttgagt
    12301 accgacaaag ccagctgcct caaccgtaac tgttactact atcgtgaatg cccgtttttt
    12361 gttactcgtc gggagattca ggaagcggaa gtggtggtgg caaaccatgc gctggtgatg
    12421 gcggcgatgg aaagcgaagc cgtactgcct gacccgaaaa atctgctgct ggtgctggac
    12481 gaaggtcatc acctgccaga tgtggcccgg gatgcgctgg agatgagcgc tgaaatcacc
    12541 gcgccctggt atcgactaca gctggacttg ttcacgaaac tggtcgctac ctgcatggag
    12601 cagtttcgcc cgaagaccat cccaccgctg gcgatccctg aacgtttgaa tgcgcattgt
    12661 gaagagttgt atgagcttat cgcctcgtta aacaacattc tcaatctcta catgcctgcc
    12721 gggcaggagg cagagcaccg ttttgcgatg ggcgaactgc ctgatgaatt gctggagatc
    12781 tgccagcggc tggcaaaact caccgagatg ctgcgtggtc tggcggagtt atttcttaac
    12841 gatttaagtg agaaaaccgg cagccacgat attgtgcgtc tgcatcggtt gattttacag
    12901 atgaaccgcg cgctggggat gtttgaggtg caaagcaaac tctggcggct ggcgtctctg
    12961 gcgcaatctt ccggtgcgcc ggtgaccaaa tgggcgacgc gggaagagcg cgaagggcag
    13021 ctacacctct ggtttcactg cgtgggaata cgtgtcagcg atcagctgga aaggctgctg
    13081 tggcgcagta ttccgcacat tattgtcacc tccgcaacct tgcgttcgct gaatagtttt
    13141 tcgcgtttgc aggagatgag tggtctgaaa gagaaagcgg gcgaccgttt tgtggcgctg
    13201 gattccccct ttaaccactg cgaacagggc aaaattgtta ttccccggat gcgctttgag
    13261 ccttccatcg acaacgaaga gcaacacatt gccgaaatgg cggccttttt ccgtgaacag
    13321 gtggagagca aaaaacatct cggtatgttg gtactgtttg ccagcggacg ggcgatgcag
    13381 cgctttctcg actatgtgac ggatttacgc ctgatgttgc tggtgcaggg cgatcagccg
    13441 cgttatcgct tagttgaact gcaccgcaaa cgcgttgcca acggtgagcg tagcgtgctg
    13501 gtgggcttac agtcatttgc cgaagggctt gatttgaaag gtgatctgct cagccaggtg
    13561 catatccaca aaatcgcttt tccgcccatc gacagcccgg tggtgatcac cgaaggggaa
    13621 tggctgaaaa gcctcaaccg ctatccgttt gaggtgcaaa gcctgccgag cgcctcgttt
    13681 aacctgattc agcaggttgg gcgactgatt cgaagccacg gttgctgggg cgaagtggtt
    13741 atctacgaca aacgcttgct gacaaaaaat tatggcaagc gactactgga tgcattaccg
    13801 gtatttccga tagagcaacc ggaagtccct gaaggtatag ttaaaaagaa agaaaaaacg
    13861 aaatccccac gccgtcggcg gcgttaatga tgtgagtcag gtaaggagtc gtaaatggac
    13921 tatcgcaaaa taattaaaga gatcgggcgc gggaaaaacc acgcgcgcga tttagaccgg
    13981 gatactgcgc gcggtctgta tgctcatatg ctcaatggtg aagtccctga ccttgagttg
    14041 ggcggcgtat tgattgcgct gcgtatcaaa ggggaagggg aaggggaagc agagatgctc
    14101 ggcttttacg aagccatgca aaatcacacc atcaagctga cgccgcctgc aggcaagccg
    14161 atgccgattg tcatccccag ttacaacggc gcgcgtaaac aggctaacct gacgccatta
    14221 ttggcgattc tgctgcataa actcggtttt cctgtggtgg ttcacggagt tagcgaagat
    14281 ccaactcgcg tgctgacaga aaccattttc gaactgatgg gtattacacc aacgttgcac
    14341 ggcgggcagg cgcaggcgaa gctcgacgaa catcaaccgg tgtttatgcc tgtcggcgcg
    14401 ttttgcccgc cgctggaaaa acaactggcg atgcgctggc ggatgggcgt gcgtaacagt
    14461 gcacataccc tggcgaaact ggcgacgcca tttgccgaag gtgaggcact gtgtctttcc
    14521 agcgtttcgc atccggaata cattggacgc gtcgcgaagt tctttagcga tatcggcggg
    14581 tgggcgttac taatgcatgg tactgaaggc gaagtgtatg ctaatccgca gcgctgcccg
    14641 cagatcaatc tcattgaccg tgaagggatg cgggtgctgt atgaaaaaca ggaccctgct
    14701 ggtagcgagt tactgccaca agcaaaagat ccggaaacca cggcgcagtg gactgagcgt
    14761 tgcctggctg gcagcgaagc gattcccgaa tcgctgaaaa tccagatggc ttgctgcctg
    14821 gtggctacgg gtgaagtggc aactatcagc gatggcctgg cgcgcgttaa tcaggcattt
    14881 taatttttat ctcagccgga tgagccacgt catccggctt ttaccccacc gcaatatgaa
    14941 attcctgcat ctttattgac cttcccgcgc ccggcgtgca gcataaaaaa acaacaaaca
    15001 cataacataa acaggagtta accatggaaa gtggtcatcg ctttgatgct cagacgctgc
    15061 acagttttat tcaggctgta tttcgtcaga tgggtagcga ggaacaagaa gcgaaattag
    15121 ttgccgatca tttaatcgcg gcaaacctgg cagggcatga ttctcatggt attggcatga
    15181 tcccaagcta tgtgcgttcc tggagtcagg ggcatctgca aattaaccat catgccaaaa
    15241 tcgttaaaga ggcgggggcg gcagtcacgc tcgatggcga tcgcgcattc ggtcaggtcg
    15301 cggcacacga agcgatggcg ctggggattg agaaagcgca tcagcacggt attgctgccg
    15361 tggcgctaca taactcgcat catatcggcc gtatcggtta ctgggcggag cagtgttcag
    15421 cggcggggtt tgtctctatc cactttgtta gcgtggtcgg tattccaatg gtcgcgccgt
    15481 tccacggtcg cgacagccgc tttggcacca atcccttctg tgtggttttc cctcgtaaag
    15541 ataatttccc gctgttgctt gattacgcca ccagcgccat tgcatttggc aaaacccgcg
    15601 tcgcctggca taaaggcgtc cccgtgccgc caggttgcct gattgacgtt aacggcgtgc
    15661 cgacgaccaa tccggcggta atgcaggagt cgccgttggg gtcgctgttg acctttgccg
    15721 aacataaagg ctacgccctt gcagcgatgt gtgaaattct tggcggggcg ctttctggcg
    15781 gtaaaacgac gcatcaggaa acgttgcaaa ccagccccga tgccattctt aactgcatga
    15841 ccactatcat catcaacccg gaactgttcg gcgcgccgga ttgtaacgcg cagaccgaag
    15901 cctttgccga gtgggtgaaa gcctcgccgc atgatgacga taagccgatt ttgctaccgg
    15961 gcgagtggga agtgaacacg cgtcgcgaac ggcaggagca ggggattcca ttggatgcgg
    16021 gaagctggca ggccatttgt gatgcggcgc ggcagattgg tatgtcggaa gagacgttgc
    16081 aggctttctg tcagcagtta gccagctaaa aaaaagcccg tccagtggcg gacgggcaaa
    16141 caagggtaac ataggatcaa tgagggttag agcatatgcg tctgtcggca aacagacagg
    16201 gaaatacttg tgctggacgt agcgtaaacg cctgatccgt ccaacggttc ggattttgta
    16261 ggcctgataa gacgcggtag cgtcgcatca ggcatctgtt gccggatgcg gcataaacgc
    16321 cttatccgtc ctacgaatta cttataaatt accgcagtac cgcttaattt attgttgttg
    16381 gtggcggaag tgatgctgta tccactggca ccggctgccg ccgctttctc agccagtttc
    16441 gcttccaggg catcgagggt ggatgcgcca tcggcagaaa ccacgccgat tttattcatg
    16501 ttctgtgcct gggatgccgt taccggttcc gccgcgaaca cggcaaatga cagagttgaa
    16561 agagccatag cagcaacaac agtattgata gttttcataa ttaatctctc gcaggtgatt
    16621 tttgtataag tgacgttgtt tcgtcgatgt gatgagtatc acgttttttt acgagagata
    16681 aaatcgaaga gaattgaccg ccttgttcaa ataaattgat tgatatctaa catacagata
    16741 aaccatttat ttgttgtact tatggacttg ttttatgccg cgtggcagtc acagcagcgt
    16801 taaaggcacg gaatgccacg caaaatacaa attatactcg ctatgcatct cgccagttgt
    16861 agttaacgta actggctgtc tttcgaacct cagcgattat atcctgtata cgctggtttt
    16921 tgtaaatctt tctcctgctg acaatgaata cataagcgca cgccaggaat ggcttcccga
    16981 cgggcttgcg ggatgggggc accgcactct tcacattcat acaggctttc gccacgcgga
    17041 atttcacccc gagcgcgggc aatcgcatct tcaattgtac tgttgatctg ttcgttgacg
    17101 gcgtcatcgt tagcccaagc ggatgccata tcgtcctccc catatcaatg cttgtacagt
    17161 taagtgtagc taatataggg acgaacctgg gcagttcaag cgtcagatct ccgaccattc
    17221 ccgcagcaga ttatgataaa gattgagcag cgacaggatc tcttcatttt caccatagcg
    17281 gcttttcagc gactgaatat ttttgtccag ttcaaacagc atggcgcgct ttttatcatc
    17341 gcggatcatc gactggatcc acataaatga tgccactcgt acgccgcggg ttacgggtgt
    17401 cacgcaatgc aggctgctgg agggatacaa caccagatca cctgccggga gttttacccg
    17461 atgttgtcca aaggtgtcat taacgaccag ttcgccgccg tcgtagcttt gtggatcgct
    17521 taaaaacagc gtggcagaaa ggtcagtgcg catccagccg ttttgtggat ggctgcgtac
    17581 tgcgccatcc acatggaaac cataggtttc attgttctga tagcgattaa acagcggcgt
    17641 ggaaagggta cgcggcaagg cagcggcaaa gaacaatgca tgttggttaa ccgcgttcag
    17701 cacctcattt tgcagtgcgg cgtataacgc gctgcgggtg tctacttgct gattgttctt
    17761 aacttgtgca ccttgtgctc cggtggtgac gcgtccatcc acccattcgg cttgttccag
    17821 ttgttcgcga aaatgggcga cgtcctgtgg cgataacacg ccgggaatgt ggtacatcat
    17881 acaagttctc caaaaagtgg ggcctgcgcc ccacatctga atcagaaatg catattggct
    17941 gtgagcaaga aggttcttgg ctcgcccggg tgataacggt agccgctctt gttgattgag
    18001 gcgacgtaat cggtatcaaa caggttgtaa acgtttagct ggaagtcgag attgcgatta
    18061 acccgatacc ccagtttggc atctgcgacc cagtaacctt ctgtaaatgc tggcgttccc
    18121 accgcgccgt ctgaaccttt atgtatactg ccgatatagc gtgcgcccgc gccaacagag
    18181 atatcatcgg ttgcctgata ttggctccat aaggtgaagg cgtgctccgg ggtatacggc
    18241 agcgatgagg aaccatcctg ggcaacatct ttgccgtttt tgatggttgc tttttgctgg
    18301 gtatagccgc caatcacctg ccacgcggga gtgatattcc ctgccacgga tatctcatag
    18361 ccttcgacgc gtttcttacc gtattgcgag taagtcccgt catcattttg ctcaacttca
    18421 ttttcgatat cagtacggaa cagcgcggcg gtgagcaaca ggcgtttatc cagaacctgc
    18481 catttggtgc caatctcgct ggtgttggct ttttgcggtt taaaatcggt gcggttggca
    18541 ctgttaccac tgccagactg cgcaagggcg aagttgttgc cgcccggagg ctgctgggaa
    18601 acggcatagt taatatagac attgccgttt tccgtcaggt gatacagcgc cccggctttc
    18661 cagttcacca gattgcccga cttggcggtg tcgacggtgg tgactggaga accttttgcc
    18721 acaccagctg ggcaggtgat ggcaccgcgt ccgctgccgc cgcaggtggt ggcactgtca
    18781 tattcagtat gataattatc cagacggatc ccgccgttca gctcaaaatc acgggtgatt
    18841 tgcagcgtat cgaaggcgta aattgcgaag gtatccgtct gaccattggc gtttgcgccg
    18901 ttgcgcgtca ggccgccggg atgaattctg ctgtcaggat gataaatatt taccgccggt
    18961 aacgtcaccg gattaacgcc atagttagtc tgcgtttcac gggtaaattc cacgccggta
    19021 ctgacatcat gaccgataga accggtatag aacgtcgagg tcaggttggt ctggttggtg
    19081 agaattttat tactcacatc tttgatattc gccgtgcgtg actaggtcca gctattcaca
    19141 tcgctggtgg gctgagtaat attcgacgcc ccgcccataa tcgccgtcat caggtaatcc
    19201 tgctttacgc gcgaccaacg ggtagtattg cgaatggtgg tgttatcgtt gatgtcgtgc
    19261 tcaaaacgca tggtggcggt gtcggtggtc gaatcgtcgt aatcggaatc cgtgccgtaa
    19321 aagttatgag tatcaacttt tccggaatga ttcagggccg ccgctcccgc agatggggca
    19381 gaatagcccg gcaaaccgat ggtcggaatg ccgccgtctg gcgtgttgtg ctgagtgaca
    19441 tgcagataat taagatacaa acgattcgct gtaccaaggc caaaagcgac agaaggggcg
    19501 acgccgtaac gctcattttt gactttgtcg cgtccggcat cgtgggtttt ttcgcccatc
    19561 acattcaggc gcaccgcggt agtatcacca atgacctgat tgacgtccag cgtgccgcgg
    19621 cggaaccagg cgctgccaat actggcggag gcgtcaatgc cggaatcatt gcgcggctgc
    19681 ttgctgatca tattgatcga gcctgtcggt gcgctgcgcc cgtagtcggt gccggacggc
    19741 cctttaatca cttcgacctg ctcggtattg aaggtgtcgc gcgagacgct gccgatatcg
    19801 cgaataccat caatataaat actgttagag gtatcggcac cacgcatata aatggcgtcg
    19861 ccagtggtgg agttaccgtt ctcacccgca aaaaacgcac ccacgc
//
LOCUS       DAETPZ010000072        19577 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.072, whole genome shotgun sequence.
ACCESSION   DAETPZ010000072 DAETPZ010000000
VERSION     DAETPZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.072"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(231..2060)
                     /gene="glmS"
                     /locus_tag="K0W59_002883"
     CDS             complement(231..2060)
                     /gene="glmS"
                     /locus_tag="K0W59_002883"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBI3023841.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2222..3592)
                     /gene="glmU"
                     /locus_tag="K0W59_002884"
     CDS             complement(2222..3592)
                     /gene="glmU"
                     /locus_tag="K0W59_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBI3023842.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGPLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3944..4363)
                     /gene="atpC"
                     /locus_tag="K0W59_002885"
     CDS             complement(3944..4363)
                     /gene="atpC"
                     /locus_tag="K0W59_002885"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBI3023843.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4384..5766)
                     /gene="atpD"
                     /locus_tag="K0W59_002886"
     CDS             complement(4384..5766)
                     /gene="atpD"
                     /locus_tag="K0W59_002886"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBI3023844.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5793..6656)
                     /gene="atpG"
                     /locus_tag="K0W59_002887"
     CDS             complement(5793..6656)
                     /gene="atpG"
                     /locus_tag="K0W59_002887"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBI3023845.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6707..8248)
                     /gene="atpA"
                     /locus_tag="K0W59_002888"
     CDS             complement(6707..8248)
                     /gene="atpA"
                     /locus_tag="K0W59_002888"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBI3023846.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8261..8794)
                     /gene="atpH"
                     /locus_tag="K0W59_002889"
     CDS             complement(8261..8794)
                     /gene="atpH"
                     /locus_tag="K0W59_002889"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBI3023847.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8809..9279)
                     /gene="atpF"
                     /locus_tag="K0W59_002890"
     CDS             complement(8809..9279)
                     /gene="atpF"
                     /locus_tag="K0W59_002890"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBI3023848.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKLRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9341..9580)
                     /gene="atpE"
                     /locus_tag="K0W59_002891"
     CDS             complement(9341..9580)
                     /gene="atpE"
                     /locus_tag="K0W59_002891"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HBI3023849.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9627..10442)
                     /gene="atpB"
                     /locus_tag="K0W59_002892"
     CDS             complement(9627..10442)
                     /gene="atpB"
                     /locus_tag="K0W59_002892"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBI3023850.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10451..10831)
                     /gene="atpI"
                     /locus_tag="K0W59_002893"
     CDS             complement(10451..10831)
                     /gene="atpI"
                     /locus_tag="K0W59_002893"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HBI3023851.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11448..12071)
                     /gene="rsmG"
                     /locus_tag="K0W59_002894"
     CDS             complement(11448..12071)
                     /gene="rsmG"
                     /locus_tag="K0W59_002894"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBI3023852.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(12135..14024)
                     /gene="mnmG"
                     /locus_tag="K0W59_002895"
     CDS             complement(12135..14024)
                     /gene="mnmG"
                     /locus_tag="K0W59_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBI3023853.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14403..14846)
                     /gene="mioC"
                     /locus_tag="K0W59_002896"
     CDS             complement(14403..14846)
                     /gene="mioC"
                     /locus_tag="K0W59_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HBI3023854.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14936..15394)
                     /gene="asnC"
                     /locus_tag="K0W59_002897"
     CDS             complement(14936..15394)
                     /gene="asnC"
                     /locus_tag="K0W59_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HBI3023855.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15546..16538
                     /gene="asnA"
                     /locus_tag="K0W59_002898"
     CDS             15546..16538
                     /gene="asnA"
                     /locus_tag="K0W59_002898"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBI3023856.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPVAVRESVPSLL"
     gene            complement(16543..17994)
                     /gene="viaA"
                     /locus_tag="K0W59_002899"
                     /pseudo
     CDS             complement(16543..17994)
                     /gene="viaA"
                     /locus_tag="K0W59_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
     gene            complement(17988..19484)
                     /gene="ravA"
                     /locus_tag="K0W59_002900"
     CDS             complement(17988..19484)
                     /gene="ravA"
                     /locus_tag="K0W59_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HBI3023857.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLKASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4683 a         5169 c         4912 g         4813 t
ORIGIN      
        1 aataacggca ctggggcaac gaaggggcga ttgcccggca gcatgatgtc caggcgattc
       61 acaatctggt gaagaggttc tttacgctca agcgcctgtc caatgtgcat aaaaaaatcc
      121 ggaaacaagt gagcgtttcc ggattcttac acagccactg gatcggtcaa ctgatcctta
      181 actgatcggc attacaccgg tcggcagccc ttttttatct atgatagtga ttactcaacc
      241 gtaaccgatt ttgccaggtt acgcggctgg tcaacgtcgg tgcctttgat cagcgcgaca
      301 tggtaagcca gcagctgcag cggaacggtg tagaagatcg gtgcaatcac ctcttccaca
      361 tgcggcatct cgatgatgtg catgttatcg ctacttacaa aacccgcatc ctgatcggcg
      421 aagacataca attgaccgcc acgcgcgcga acttcttcaa tgttggattt cagtttttcc
      481 aacagttcgt tgttcggtgc aacgacgata accggcatat ccgcgtcaat cagcgccagc
      541 gggccgtgtt tcagctcgcc tgcagcgtag gcttcagcgt gaatgtaaga gatctctttc
      601 agcttcaatg cgccttccag cgcgattggg tactgatcgc cacggcccag gaacagcgcg
      661 tgatgtttgt cagagaaatc ttctgccaga gcttcaatgc gtttatcctg agacagcatc
      721 tgctcaatac ggctcggcag cgcctgcaga ccatgcacaa tgtcatgttc aatggaggca
      781 tccagacctt tcaggcgaga cagcttcgcc accagcatca acagcacagt taactgagtg
      841 gtgaatgctt tagtggacgc cacgccgatt tccgtacccg cgttggtcat cagcgccaga
      901 tcggattcgc gcaccagaga agaacccgga acgttacaga ttgccagcga accaaggtaa
      961 cccagctctt tcgacagacg caggcccgcc agggtatccg cggtttcgcc agactgtgac
     1021 aaggtgatca tcaggctgtt acgacgcacg gcagatttac gatagcggaa ttcagaggcg
     1081 atttcgacgt cgcacggaat acctgccagc gattcaaacc agtagcggga aaccataccg
     1141 gagttataag aagtaccaca ggcgaggatc tgaatatgct caaccttcga cagcagttcg
     1201 tcggcgttcg gtcccagctc gcttaaatca acctgaccgt ggctgatgcg cccggtaagg
     1261 gtgtttttta tcgcgttcgg ctgttcgtag atctctttct gcatgtagtg acggtaaata
     1321 cctttatcgc ccgcgtcata ttgcagattg gattcgatat cctgacgttt tacttccgcg
     1381 ccagttttat cgaagatgtt taccgaacgg cgagtgattt ccgcaatatc gccctcttca
     1441 aggaagataa agcgacgggt caccggcaac agcgccagct ggtcagaagc gataaagttt
     1501 tcgcccatac ccaggccaat caccagcgga ctaccagaac gtgccgccag cagggtatcc
     1561 gggtgacggg agtccatgat cactgtaccg tacgcaccgc gcagctgcgg gatagcacgc
     1621 agaacagcct cacgcagagt cccgccttgt ttcagctccc agttcaccag atgggcaatc
     1681 acttcggtgt cggtttcaga aacgaaggta tagccacgcg cttttaactc ttcacgcagc
     1741 ggttcatggt tttcgatgat gccattatgc accaccacaa tgtgttcaga aacatgcgga
     1801 tgcgcgttag cttctgaagg ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg
     1861 ccgccatgca gaggatgttc ttccgctgcc tgagccagca tctggacttt accgaggcga
     1921 cgcaggcgag tcatatgacc ttccgcatca acaacggcca gaccggcaga gtcatatccg
     1981 cggtattcca gacgacgtaa accttcaaga aggatttctg ctacatcacg ttgcgcgatc
     2041 gcgccaacaa ttccacacat agtttttgat tccgatttat atcgttgtcg gtcaacctgt
     2101 atgcccgttt tttcgggcgc cccgagcctt gtagagagtg gggttatttt tatagttact
     2161 gcttgtggga gggagattat gttatctcct catcccagat gaccggatta cccggccaga
     2221 attacttttt ctttaccgga cgacgccagc cttctttctg agtctgcggc acacggctga
     2281 tagctaatgc attttcgccg acattacgcg tcacagttgt acccgcagca atggtcgcgc
     2341 ctttgcctac tgttaccggg gccaccagct gagtgtcgga accgacaaac acatcgtcgc
     2401 cgataatggt cttaaattta ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga
     2461 tgttaacgtt atcgccaatt tccgcatcgc ctaggtaagt cagatgcccg gctttcgtgc
     2521 ctttacccag acgcgctttt ttcatctcaa cgaagttacc aacgtgtgca ccttccagca
     2581 actcagcacc aggacgcaga cgggcaaacg ggccaatagt acaggccgct gccagattcg
     2641 cgtcttccac aacggtatac ggactgattt cgcaatcatc gccaatcacg ctgtttttaa
     2701 tcacgcaacc ggtgccgatt ttcacgcgat gaccgagagt cacgttgccc tcgatgataa
     2761 cgttagtatc aatttcaaca tcgcgcccgt gagtaagcgg gccgcgcaga tcaaaacgcg
     2821 ccggatcgcg cagcataacg cctgctaaca gcagtttttc agcctgttcg gactggtaaa
     2881 cgcgctccag acgggagagt tgcaggcggt tattcacacc ttctacttcg cttaaacgtt
     2941 gcggatgaac ggcgacgatt tcacgccctt cctgatacgc cagcgcaata atgtcggtga
     3001 tgtagtattc gccctgagca ttattgttgg tcagcttcgc cagccagcgt ttcatatctg
     3061 cgccgttggc aatcagaatg ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg
     3121 catctttgtg ctcaacaatg ccggtaactt tgccattttc acgggtgata cgtccataac
     3181 cggtcggatc atccagtttc accgtcagca gaccaatgcc accctggggt ttagcatcac
     3241 gcaggcgctg gagtgtttcg acagagatca gcggcacgtc gccgtagagc attaaaatgt
     3301 cttcatcatc ggcaaagaaa ggtgcggcct gctgcattgc atgacccgta cccagctgct
     3361 ctgcctgaag cacccagtta aggttgtcgt ctttcaacgc ctgtttaagc agatcgccgc
     3421 cgtgaccgta caccaggtga acgtgcgctg cgcctaattc attcgcagca tcaatgacat
     3481 gctgaaccat cgctttcccg gcaagggtat gcagcacttt cggaagatcg gaatacatgc
     3541 gcgtgccttt gcctgcggca aggatcacta cgctcatagc attattcaac atacgcgtcc
     3601 tgactgtaat ttgagaacga atttaaaccg cttcaccttg aaaaaactac atttttttca
     3661 tcgtgaaatg gacagaggat aaattgttca ataacggatt atccccgctg acgaaagtgc
     3721 cattttcgac catcgtcacg ccttttgtct gcagaaaatt aaggcagtac cttcatttaa
     3781 gcgtcaggtg gatgtttttg ctcttatttc gatcaatgaa taaacgggaa aacaggggtg
     3841 ttttatatca gcaggatcta tgtgaacgct cttcaggacg ggtcaacgcg taaaaaaaag
     3901 ccagcctgtt tccagactgg cttttgtgct tttcaagccg gtgttacatc gcttttttgg
     3961 tcaactcgat aacgcgcagc tgcgcgatcg ctttggccag ttccgcagac gcctgagcgt
     4021 aatctacgtc gccgtgagag ctgctaatgt gctcttcagc cttacgtttc gcttccatgg
     4081 ctcgcgcttc gtcgagatcc tggccgcgaa ttgcggtgtc ggccagaacg gtcacgttgc
     4141 caggctgcac ttcaagaatg ccgccagaca gatagataaa ctcttcgtga ccgtgctgtt
     4201 tcacgatgcg aatcatacca ggcttaatgg cggtgagcag cggtgcgtgg ccagggtaga
     4261 tccccagttc accttcgcta cccgttacct ggattttctc gaccagacca gagaacattt
     4321 gttgctctgc gctgacgacg tccaggtggt aagtcattgc catatcaccc tccgattaag
     4381 gcgttaaagt tttttggctt tttccacagc ttcttcgatg gaaccgacca tgtagaacgc
     4441 ctgctccggc aggtgatcgt attcgccttc catgatgcct ttaaagccac ggatggtgtc
     4501 tttcagggag acgtatttac ccggagaacc ggtgaatact tctgccacga agaacggctg
     4561 ggacaggaag cgctggatct tacgagcacg cgctaccacc agtttgtctt cttcagacag
     4621 ttcatccata cccaggatgg cgatgatgtc tttcagttcc tgataacgtt gcaggatgga
     4681 ctgaacgcca cgcgcggtgt cgtagtgttc ctgaccaacc accagcgggt ccagctgacg
     4741 gctggtggag tccagcgggt caacggccgg gtagataccc agagacgcga tctgacggct
     4801 cagtaccacg gttgcgtcaa ggtgcgcaaa ggtggttgcc ggagacgggt cagtcaagtc
     4861 atccgcaggt acgtataccg cctgtacgga ggtgatagaa ccagttttgg tggaggtgat
     4921 acgttcctgc agaacgccca tctcttccgc cagggtcggc tgataaccta ccgctgaagg
     4981 catacggccc agcagtgcgg atacttccgt accggccagg gtgtaacgat agatgttgtc
     5041 aacgaacagc agaacgtcac gaccttcgtc acggaatttc tcagccatgg tcagaccggt
     5101 cagagcaacg cgcagacggt ttcccggcgg ctcgttcatc tggccataca ccagggatac
     5161 tttgtcgata acgttggagt cggtcatttc gtggtagaag tcgttaccct cacgagtacg
     5221 ttcacctacg cccgcaaaca cagagtaacc ggagtgctcg atcgcgatgt tacgaatgag
     5281 ctccatcatg tttacggttt tacctacacc cgcaccaccg aacagaccaa ctttaccgcc
     5341 cttagcgaac ggacacatca ggtcgataac tttgataccg gtttccagca gttcctgaga
     5401 gtttgacagc tcttcgtagg aaggtgctgc gcggtgaatc gcccaacgct cttcttcacc
     5461 gatctcgcct ttcatgtcga ccggttcacc cagtacgttc atgatacggc ccagagtcgc
     5521 tttacctacc gggacttcga tcgggtgttc gaggtctttt acatccagac cgcgacgcag
     5581 accgtcggag gaacccattg cgatggtacg cacgataccg ccgccgagct gctgctgaac
     5641 ttccagcacc agacgctcat taccattttg cacctcaaga gcatcgtaca cgcgcggtac
     5701 ggcatcctga gggaattcga cgtcaactac ggcgccgatt acctggacaa tctttccagt
     5761 agccatctta aatcctctac gaaataacct gtttaaaccg cggcggcccc cgagacgatc
     5821 tcggtgagtt cctgagtaat gctggcctga cgagctttgt tgtataccaa ctgcagctct
     5881 ttaatcaggc tgccgccatt gtcggtcgcg gctttcatcg ccaccatacg ggcggcctgc
     5941 tcgctggcca ggttttcaac cacgccctga taaacctgag attcgacata acgacgcagc
     6001 agggtatcca gcaacgcctt cggatcgggt tcgtacaggt aatcccagga tttatgtttc
     6061 agatcatcat catctgatgc cggtaacggc agcagctggc tgatggtcgg aacctgagac
     6121 atggtgttaa taaatttgtt gctgacaatg taaagcttgt ccagacggcc ttcgtcgtag
     6181 gcctgcaaca tcacttttac cggaccgatc agttcggaca gggaagggtt atcccccatg
     6241 ccggtgactt gggcaacaac attgccgccc acggagttga agaacgacac gcctttcgag
     6301 ccgatcattg cgaggtcgca ttgaacgcct ttgtcggtcc aggtcttcat ttccgccagc
     6361 agttttttga acaggttaat gttcaaacca ccgcacaaac cacggtcggt cgacaccacc
     6421 aggtagccca cgcgtttaac gtcgcggtct tccaggtaag ggtgcttata ttccagatta
     6481 ccgtgtgcaa ggtgaccaat cactttgcgc atggtttctg cataaggacg gctggccgcc
     6541 atgcgatcct gcgatttacg cattttggaa gcggcgacca tctccatcgc tttagtgatc
     6601 ttttgcgtgt tctggacgct tgcgatctta ctacgtatct cttttgcgcc ggccatgagc
     6661 ttctcctcaa tgccttgcgg cctgccctaa ggcaagccgc cagacgttac caggattggg
     6721 ttgctttgaa ggaatcgagg atgcctttca gcttgccttc gatttcgtcg ttgtagccac
     6781 cggtctggtt gatctcttgc atcaacggag cgtgatcacg gtcgacgtaa gccagcagag
     6841 cggcttcgaa gctgccaatt ttcgacagtt caacatccgc caggtaacca cgttctgctg
     6901 cgaacagaac cagagactgc tgcgcaacgg acatcggcgc atactgtttc tgtttcagca
     6961 gttcggtcac tttctgaccg tggtcaagct gcttacgtgt tgcatcgtca aggtcggatg
     7021 caaactgaga gaacgctgcc agttcacgat actgtgccag agcggtacgg ataccaccgg
     7081 acagtttttt catgatcttg gtctgtgctg caccaccaac acgggatacg gaaatacccg
     7141 ggttaaccgc aggacgaata ccggcgttga acaggttggt ttccaggaag atctgaccat
     7201 cggtaatgga gattacgttg gtcggaacga acgcagaaac gtcacccgcc tgagtttcga
     7261 taatcggcag tgcggtcaga gaaccggttt tccctttcac ttcacctttg gtgaaggctt
     7321 caacgtattc ggcgttaaca cgtgcagcac gctccagcag acgagagtgg aggtagaaaa
     7381 cgtcgcccgg gaatgcttca cgtcctggcg gacgacggag cagcagggag atctgacggt
     7441 aagcaacagc ctgtttagac aggtcatcgt aaatgatcag cgcatcttca ccgcggtcac
     7501 ggaagtattc gcccattgcg caaccggcat acggtgccag gtattgcagt gcagcggatt
     7561 cagacgcggt tgctaccaca acgatggtgt tagccagtgc gccgtgctct tccagtttac
     7621 gtaccacgtt agaaatggtg gacgctttct ggccgatagc gacatagata catttgatac
     7681 cggaatcgcg ctggttgatg atggcatcga tagccagggc ggttttacct gtctgacggt
     7741 caccgatgat caattcacgc tgaccacgac cgattgggat catggagtca acggctttat
     7801 aaccggtctg taccggctga tctacggact gacgttcgat aacgcccgga gcgattgctt
     7861 ctacagcaga gaagccgtcg tgatccagcg gacctttacc gtcgattggt gcacccagag
     7921 tgttaaccac acggcccagc aggccacggc caaccggaac ttccaggata cggccagtac
     7981 acttaacttt catgccttcg gcaaggtcag cgtacggacc cataacaacc gcacctacag
     8041 agtcgcgctc gaggttcagt gcgatagcgt aacggttacc cggcagggag atcatttcac
     8101 cctgcataca atcggccagg ccgtgaatgc ggataacacc gtcacttaca gaaacaatag
     8161 taccttcgtt gtgagcttca ctcacaacat tgaactgagc aatgcgctgc ttgatcagtt
     8221 cgctgatttc ggtggaattc agttgcatgc tccagtcccc ttaagactgc aagacgtctg
     8281 caaggcgctc aagacgaccg cgtacgctgc catcaatgac catatcaccc gctcggatga
     8341 taacgcctgc cattacagac ttatcgattt tgcaattcag cttaactttg cgtgacagac
     8401 gtttttccat cgcagcagaa attttcgcga gctgttgttc actcagtgcg gcagcggaaa
     8461 tgacgtctac ctcagcggta gcctcactca cggcacgaag gtgaataaac tgctccagaa
     8521 catccgggag cgcgttaaga cgaccatttt cagccattac ccgaatcagg ttctgaccgt
     8581 tttcgtccag ttgctcacca caaactgcga taaacgactc ggcgagcgtt tctggcgcaa
     8641 gcgcgccaga gagaagctct gccatttgtt cgtttttggt tacctcggcg gcaaacgcca
     8701 gcatgtcctg ccagcgttct acactttggt gttcgacggc aaagtcaaaa gctgctttgg
     8761 cgtaggggcg agctaccgta ataaattcag acatcagccc ctccctcctt acagttcagc
     8821 gacaagttta tccacgatgt cgctgttagc agcttcatcc acggaacgtt cgatgatctt
     8881 ctcggcgcca gcaacagcca ggatagcaac ttgcttacgc agctcttcac gggcacgttt
     8941 acgctcggct tcaatttctg cctgcgcctg ggccacgatt ttagtacgtt cctgttctgc
     9001 ctcagctttc gcttcgtcca gaatctgcga gcggagttta ttcgcctgct cgatgattac
     9061 ctgggcttcc gctttcgctt ttttcagctg gtcggtcgcg ctggcctttg caaggtcaag
     9121 gtccttatgt gctcgttctg cggaagcaag gccgtcagca atttcttttt gacgtttttc
     9181 gatggctgcc attaatggcg gccatacgta cttcatgcag aacagaacga acaggacaaa
     9241 cgcgatggcc tggccgagga ttgttgcgtt aagattcaca gcacaatgcc tctatttagt
     9301 taacgttctg atattgctct ttaaataaaa gcaacgctta ctacgcgaca gcgaacatca
     9361 cgtacagacc cagacctaca gcgatcatcg ggatagcatc caccagaccc ataacgataa
     9421 agaactgagt acgcagcaga ggaatcagat caggttgacg cgctgcgcct tccaggaatt
     9481 tacccccgag gatgccgata ccgatcgcag caccgattgc cgccagaccc atcatcacag
     9541 cggcagccat gtacagcaga tccatattca ggttttccat gacagtctcc agtttgtttc
     9601 agttaaaacg tagtagtgtt ggtaaattaa tgttcttcag acgccatcga cagatagacg
     9661 atcgtcagaa ccatgaagat gaaggcttgc agcgtaatga tcaggatgtg gaaaatggcc
     9721 cacggcacat tcaggatcca ctgtgaccac cacggcaaca gaccagcaat cagaatgaaa
     9781 atcagctcac cggcatacat gttaccgaac agtcgcaaac cgagtgaaac tggtttggac
     9841 agcaggctta ccccttcaag gattaagttg acaggaatga acgcccagtg attgaacggc
     9901 tgcagcgtca actctttcgt gaagccgccg atgcctttca ttttgatgtt gtagaacaga
     9961 atcaggataa atacgcccag tgccatagac agcgttacgt tcacgtccgc agacggaacc
    10021 acacgcagtg caggcagacc cagtacatgt tcagcaatgt acggcagcaa gtcgataggc
    10081 agtaaatcca tcagattcat caggaatacc cagacgaaga tcgtcagggc cagcggagca
    10141 atcagcttgc ttttgccatg gtacatgtct ttcacgctac cattaacaaa gccgatcacc
    10201 agctcaatcg cggtctgaaa cttacctggc acaccgctgg tcgccttttt ggctacgcta
    10261 cggaataaaa ccaagaacaa cagacccagc accaccgaga agaacatgga gtcaatattg
    10321 attgtccaga aggtggctgg ggggttttgt ggatccacca gcgagaatgt acgcaggtcc
    10381 agctgaaggt tattcaggtg gtgtcctatg taatcctgcg gcgtcatatt ttctgaagcc
    10441 atgatgcctt ttaccctttg ttgttaatta cagccggtgc cagtatctga accaccagca
    10501 ccaaaaccca cgtaacgatc agcggcaaga ataccgcctt taaaaccgcc aacgccacca
    10561 ccagtaacac caacatcgcc agaactttga aagcttcgcc aaatgcgaat gtccaagcca
    10621 cccggccttt cgctggtgta tgcgcctggt gacgccaggc aaatatcata aacaaaacgt
    10681 taggcagaaa gactgccagg cccccgctta ttgcagagac gccccagaag gggtctttga
    10741 ggctgaacag caatccactt gctatcacca ccagtaactg aacgagcaga agcttccgag
    10801 caacgtttcg actcacgagc gacacagaca tcacgttttt cactcctgct cccttcgagg
    10861 tatgccgcgt gtcgtataaa actttcttta aggcttagag tcaagcatca aaaagcggtc
    10921 aaattatacg gtgcgccccc gtgatttcaa acaataagta gccaaaaggt gaataaatgt
    10981 ttaaatattt ttccagtgca tacaattgcg acttttccgc tacccctgtt cgatcatgaa
    11041 aaactgtaaa taacgcgtaa acactggtga taaagcgtgc ttcagatcac atattgcgca
    11101 tgttcgcgca cagcatattt atttacttgg caaatgatgc ctttgcaagt ttatgatatt
    11161 tcagtctaaa aacagatact gttttaataa atgacattta cacaacaaaa accacccatt
    11221 gacattttta ataatgtttt aacagccaat gatggttctt agcgccgatt tttagcagac
    11281 tgatattttc actaatgact tattttctgc ttaccaaaaa aagccacgtt atcttgttga
    11341 tgcaaaagag tgaacgtggc gttaaatgta accagttata tcagtagaaa acctggttgt
    11401 tgttaacagt ctaaccggtc aattttttat gatttttttg ataaaaatta aattttattt
    11461 gctttaatca ccaccagatg acgttcgcca tccagggctg gaacctgaag tttaaccact
    11521 gattcgacct gatattcttc gggcaacaaa gcgatttcat cttccggcat ttgccctttc
    11581 agcgcgtaga aacggccttg ctcaccagga agatggtggc accagctcac catatcgttc
    11641 agagaggcaa aagcgcggct aattacgcca tcaaatggcg gctctgaagg aaactcttct
    11701 accctgctct gtactggttc aatattctcc agtttaagct catgttgcac ctgacgaagg
    11761 aaacgcacgc gtttaccaag gctatccaac agagtgaaat gggcttcagg acgcacgata
    11821 gagagtggaa tgcctggcag tcctggtccg gtgccgacat cgataaaccg ttcaccttgc
    11881 agatacggtg ccaccacaat gctatcgaga atatggcgta ccagcatctc attaggatcg
    11941 cggaccgaag tcaggttgta cgctttgttc catttatgca gcatattcac gtaggcaata
    12001 agctggtttt tctggtgatc agtaagcgaa atacctgcgt ctttcagcag taaggagagt
    12061 ttgttgagca cggtgattac ctgttcttga tgcgttgcct ggtaagcggg tgcttaccag
    12121 gcatttttaa tgcgttatgc gctacgacgc agcataccct gttttttcag ccacaccagc
    12181 agaatggaga tggccgcagg cgtgacgcca gaaatacgcg aagcctggcc gatagaggcc
    12241 ggtttgtgat cgttaagttt ggcgatcact tcgttagaaa gaccggatac ctggcggtaa
    12301 tccagtgtcg cgggtagcag ggtgttctcg ttacgcagct gcttttcgat ctcatcttgc
    12361 tggcgcgcga tataaccttc gtatttaacc tgaatctcaa cctgttccgc cgcctgttcg
    12421 tctgtcaacg caggggcaaa cggcgtcagc gtggttaatt tttcataagt catttccgga
    12481 cgacgcagca gatcttcacc actggcttca cgggaaagcg gcgcagtcag gtgagcattc
    12541 acttcggctg cagcttccgc cgacggggtt acccaggtcg atttcagacg ctgacgctca
    12601 cgctcgatat tctcaagttt ctcgttaaag cgcgcccaac gttcgtcatc caccaggccc
    12661 agttcacgac cgatttcagt caaacgcaga tccgcattat cttcgcgcag catcagacga
    12721 tattctgcgc gcgaagtaaa catacgatac ggttctttgg ttcctaaagt gcacaggtca
    12781 tcaaccagta cgccgagata cgcctgagaa cgtgccggag cccaaccttc tttgtcagca
    12841 gacagacggg cagcgttaag accggccagc aaaccttgcg cagcggcttc ttcgtaaccg
    12901 gtagtgccgt taatctgacc agcaaagaac agcccctgga taaacttgct ctccagcgtc
    12961 ggtttcaggt cgcgaggatc gaagaagtca tactcaatgg cataacccgg acgcacgatc
    13021 ttcgcgtttt ccatcccctg catagagcgg acgatttgca tctgcacatc gaacggcagg
    13081 ctggtggaga taccgttcgg ataaatttca ttagaggtca gtccttccgg ttcaaggaag
    13141 atctgatgct gatttctgtc tgcgaagcgc atgactttgt cttcgatcga cgggcagtag
    13201 cgtgggccga caccttcgat cacccctgca tacattgggc tacgatcgag gttactgcgg
    13261 atcacatcat gggttttctc gttggtatga gtgatataac acggcacctg ctggggatgc
    13321 agggacgcat tgcccataaa cgagaatacc ggcattgggt tatcgccatg ctgttgcgcc
    13381 agcacgctaa agtcgatggt tcgagcatca atacgcggcg gagtaccggt tttcagacga
    13441 ccaacgcgca gcggcagttc acgcaaacgg cgagagagcg gaatggatgg cggatcgcca
    13501 gcacggccac cgctgtaatt atccagaccg atatgaattt tgccgtcgag gaacgtccca
    13561 acggtgagca ccacagcttt ggcacggaac ttcagtccca tttgggtaac agcaccgacc
    13621 acgcgatcgt tttcgacaat aagatcttca accgcctgct ggaagatcat caggttcggt
    13681 tggttctcca gcgccgtacg taccgcctga cggtagcgca cacgatccgc ctgagctcgg
    13741 gtagcgcgaa ccgccggtcc tttgcttgcg tttagtatcc taaactggat acccgcttga
    13801 tcgatcgctt tcgccatcag accgccgagt gcatccactt cttttaccag atgtcccttc
    13861 ccaataccgc cgatcgccgg gttgcagctc atctgcccca gagtgtcgat attgtgtgtc
    13921 aaaagcagag tctgttgacc catacgcgcc gcagccatcg cggcctcggt gcctgcatga
    13981 cccccgccaa tgatgatgac gtcaaaagga tccggataaa acatggtgat tgcctcgcat
    14041 aacgcggtat gaaaatggat tgaagcccgg gccgtggatt ctactcaact ttgtcggctt
    14101 gagaaagacc tgggatcctg ggtattaaaa agaagatcta tttatttaga gatctgttct
    14161 attgtgatct cttattagga tcgcactgcc ctgtggataa caaggatccg acttttaaga
    14221 tcaacaacct ggaaaggatc attaactgtg aatgatcggt gatcctggac cgtataagct
    14281 gggatcagaa tgaggggtta tacacaactc aaaaactgaa caacggttgt tctttggata
    14341 actaccggtt gatccaagct tcctgacaga gttatccaca gtagatcgca cgatctgtat
    14401 acttatttga gtaaattaat ccacgatccc agccattctt ctgccggatc ttccggaatg
    14461 tcgtgatcaa gaatgttgat cttcagtgtt tcgcctgtct gttttgcacc ggaatttttg
    14521 agttctgcct cgagtttatc gatagcccca caaaaggtgt catattcacg actgccaata
    14581 ccgattgcgc caaagcggac cgcagaaaga tcgggcttct gttcctgcaa tgcttcatag
    14641 aaaggagaaa ggttgtccgg aatatctccg gcaccgtggg tggagctgat aaccagccag
    14701 atccccgagg cagataaatc ttccaacagc ggaccgtgca gcgtttcggt ggtaaaaccc
    14761 gcctcttcca gcttttcagc caggtgttct gctacatatt cggcaccgcc gagggtgctg
    14821 ccgctgataa gagtgatatc tgccataaac cgccaccttt attaagagtg gcgtattgta
    14881 cgctgtgaac gcgttgggat ctacctgtgg aaaagtatgg gattaaaaaa gccgatcagg
    14941 gcttgatggt acgcatgatt gggttttgca ggacgatcaa tgtctcggtg gactgaattt
    15001 catcaattgt ttggatcttg ttgataagta catgctggag agcgtcgatc gaacggcaca
    15061 taacttttat aaagatgctg tagtggccgg ttgtgtaata ggcttcagtg acttcatcaa
    15121 ggctttccag ctttgccagc gcggaagggt agtctttggc gctctttaat ataatgccga
    15181 taaagcagcc tacgtcataa ccgagctgct tcgggctgac atcaatacgc gccccggtaa
    15241 tgatccccgc ctgcttcatt ttctctactc gaacgtgaat cgtccccgga ctgacgccaa
    15301 attgtttcgc cagttcggcg taagcggtgc gcgcattgcc cattaatgct tccaggatgc
    15361 cacggtccag attgtcgatc agataatttt ccataggatt ttcttatgca gattgatgat
    15421 tcattctatt ttagccttct tttttaatga atcaaaagtg tgttaggttt tttattgaat
    15481 gattattgca tgtgtgtcgg tttttgttgc ttaatcataa gcaacaggac gcaggagtat
    15541 aaaaaatgaa aaccgcttac attgccaaac aacgccaaat tagcttcgtg aaatctcact
    15601 tttctcgcca actggaagaa cgtctggggc tgatcgaagt ccaggcgccg attcttagcc
    15661 gtgtggggga tggcacgcag gataacttgt cgggctgtga aaaagcggtg caggtaaaag
    15721 tgaaagctct gcctgatgcc cagttcgaag tggttcattc actggcgaag tggaaacgtc
    15781 agaccttagg gcaacacgac ttcagcgcgg gcgaagggct gtacacgcac atgaaagccc
    15841 ttcgccccga tgaagaccgt ctttctccgt tgcactcggt ctatgttgac cagtgggact
    15901 gggaacgcgt aatgggcgac ggtgagcgtc aattctcgac tctgaaaagc acggtagagg
    15961 cgatctgggc gggaattaaa gcaaccgaag ctgcggttaa cgaagagttt ggcctggcac
    16021 cgttcctgcc ggatcagatt cacttcgtac acagccagga gttactgtct cgttatccgg
    16081 atctcgatgc caaagggcgt gagcgggcga ttgcgaaaga tcttggcgcg gtattcctcg
    16141 tcgggattgg cggcaagttg agtgatggtc atcgtcacga cgtgcgcgca ccggattatg
    16201 atgactggag caccccgtca gagctgggcc atgcgggcct gaacggcgat attctggtgt
    16261 ggaacccggt actggaagat gcgtttgagc tttcttccat ggggatccgc gtggatgccg
    16321 acacgctgaa gcatcagctg gcgctgaccg gtgatgaaga tcgtctgcag ctggaatggc
    16381 atcaggcgct gctgcgcggt gaaatgccgc agaccatcgg cggcggtatc ggccagtctc
    16441 gtttgactat gctgctgctg caactgccgc atatcggcca ggttcagtgt ggagtatggc
    16501 cagttgctgt tcgcgagagc gtcccttctc tgctgtaata atttatcgcc gccagcgtct
    16561 gagcaggcgg cttcgcatcc cggtatcaaa gcgccagata tgatcgaaaa tgcgcatgat
    16621 gccgggtttg ccgtgtgccg acatcgccac ggcatgaaag cgatgctgat gtacccgctg
    16681 cagctctttc actttactcg tcacgtcgtc aggcaaccgc tgagcgataa aatcagaaat
    16741 caccaccgca tcggcatcaa accattccct gctttgcaag cgttccataa tggcgcgaaa
    16801 acaactggca agatcggtgc cgctacgaaa ctgctggctt aaaaaacgga ttgcttgttc
    16861 gatgccttgt gggcctgaaa gctcataacg gacgatctcg gtggaaaata gcataatata
    16921 acagcgccgg ttttcagcga gagcaatgcg catcaaagcc aggcagaacg ctttcgcaca
    16981 ctgttcatta aagccgccca ttgagccgga agtatccaca cagacaataa acggcccgcg
    17041 cggctgttca tcgtaatctt tatgtaccac cggacgttcg agcacttttt cacgccacga
    17101 ctcaccgtgc aggcgatagg tgagcaactg tttttccacc agccgacggt aaaactcata
    17161 ctccagttcc gttatcccta gtgtcgccag ttctggcggc aggagacgta aaatatcatc
    17221 gctttgttgc agaccatcaa cctgctcagg aaccgtcgcc ggttcgcgca ccatggtgcg
    17281 gaaggtttcc atctacgcat cgttgcgcgg tattgatttg gcttcccgag aacgccccag
    17341 ctgctctgcc aggcgtttca gttccggctg ttcgttaaga aattcaccgt atttcacaat
    17401 aaactgatag tcgccacgtt taagctgacc ggcgctcata tcccacagac gaccagctgc
    17461 ggtattgtta tctgcgagaa tcggttcaag ttgtccgctc agcgtcatgc gttcctgaac
    17521 ttcacttaac agttgttcgc gttcttcttc taatagctgt tgatttaacg tcgttgcttg
    17581 cacgatcaga cttaaacgcc aacgctggag aaaaagcgtg tgtaacgctg aagttattgt
    17641 gctgttagca tcaaccaact gccgggcttg ttctgcccat ggggaattca gacgatgcag
    17701 taagtctagg atctgtggta gctgcacaat aaactgcggc gtggagagga gctggctttg
    17761 ctgatagcac atcacctctt cggtgagttc cggcgggact cgggcatctt tcagccgact
    17821 gcgcagcgcc tcacgccagc ggggaacatc atcagtgatt gccgccttca gtcgtgggaa
    17881 tttttcaaag aagaccgcca gctgcggtga ggccagcagt gcgatgatca tctcttcgat
    17941 caatccctct tcgctgacgg ccagcatcac attaagcgta tccagcgtta gcattgttgt
    18001 gcctggcgaa tctgtgcacc gacatcctgt aggctggctt caatgcgacc taaccagtcg
    18061 ccagggataa acagacattt ttgctgttcg ctgaataaag cgtgttgctt acgccagtcg
    18121 ctttcaagct cttccagttg ttgttttatt tcacctggca gaccttcagc cgatgaaccg
    18181 ggaagtgcca gcgtactgcc ttgtaaactt acatcgcgta caacaagatg ttgggtgcta
    18241 tcaacttcca gattcagttt ctgggcaaag ccgataccgt tcagtttgcc gcgaatttca
    18301 ccacctttac tcagccactg ttccagcgcg ctacgctcaa aggagatatg aaccacttcc
    18361 atatcatgca gttttaacgg tttttgcagc agcagagtca gagtggaagc agtaacgtta
    18421 acaggaagtt gatactgctg acgacggctg aaaatgccgc ccagacgaat taacgttaag
    18481 gctgttttat cgctttgttg ctgctggagt tgcaggtgac gttgcacaat cgcgcccagg
    18541 cgggtcaaca tcccttgctg ttgccaggca tgaccggtca tcaatacatc aatttgttgt
    18601 tgtatcaaat tcaggctttg cgcgtcatac cacaggcaat ctttcagcaa aatgagatca
    18661 accggggcaa cagcactgcg accgctaaaa aaggcgctgg ccttcaataa gcggatcgct
    18721 tttttccagc gacgatccga gacataaggc gcatccggta atttatccag ttgctggcgc
    18781 agcataaaaa tcagctcaaa tacatgatcg ggcagcgtaa tttcaccaat ctctttctgc
    18841 cagcgttcat attcttcatc tgtgacctgc aaggcatcag gaaccggatt gtcgttttca
    18901 tcctgttgac tggtcagcat ggagcggaaa ttcgctttat cttgcacttt atctaaccat
    18961 agacgaatca gcatgcggtc atataacgct tccagactgc tgtctgcttc cggcagctcg
    19021 ttggaggccg ccaccagtag acgcatcggg attttttcta cgtgtgcacc gttgcggaac
    19081 tggcgctcgt taatggcggt gagcaaggta ttaagaattg ccgggcccgc tttccagatc
    19141 tcatccagaa agacgatttc tgcttccggc aggtaaccgc tggttaaacg ttcatagcgc
    19201 ccttcatctt ttagcgcctg aatagaaagg ggaccaaaaa cttcttccgg cgtggagaag
    19261 cgagtcatca gatattcaaa cgcgcgggca ttctgaaagg cgaattttaa gcgccgggcg
    19321 atcaaacttt tggcaatacc tggcgggcca aggaggaaca cactttcacc acttaatgcc
    19381 gctaataaac acaagcggat ggcgtggcta cgttcataaa gccccttttc caacgaactg
    19441 ctcaggcggg aaattctttc cgctaataaa tgagggtgag ccataatgaa gtggcgtcct
    19501 ttcgtcaaaa gttctgcgta aattgcgagt atagacgttt cttgctggtg gctaaaatag
    19561 tctcaaaggg gggtaat
//
LOCUS       DAETPZ010000073        19420 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.073, whole genome shotgun sequence.
ACCESSION   DAETPZ010000073 DAETPZ010000000
VERSION     DAETPZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.073"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..353
                     /locus_tag="K0W59_002901"
     CDS             <1..353
                     /locus_tag="K0W59_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023858.1"
                     /translation="FRKECPLNSGYLRALDNVLQELGRERTIAMSLPEFEQSLFMAAQ
                     PDNLLLATAPRYCQYYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIV
                     WLRETIKNLYASMA"
     gene            510..1259
                     /locus_tag="K0W59_002902"
     CDS             510..1259
                     /locus_tag="K0W59_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HBI3023859.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1281..1847
                     /gene="yieF"
                     /locus_tag="K0W59_002903"
                     /gene_synonym="chrR"
     CDS             1281..1847
                     /gene="yieF"
                     /locus_tag="K0W59_002903"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HBI3023860.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1901..3238)
                     /gene="adeP"
                     /locus_tag="K0W59_002904"
     CDS             complement(1901..3238)
                     /gene="adeP"
                     /locus_tag="K0W59_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HBI3023861.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLANEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3402..4067
                     /gene="yieH"
                     /locus_tag="K0W59_002905"
     CDS             3402..4067
                     /gene="yieH"
                     /locus_tag="K0W59_002905"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HBI3023862.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            4134..4607
                     /locus_tag="K0W59_002906"
     CDS             4134..4607
                     /locus_tag="K0W59_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HBI3023863.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            4656..5243
                     /locus_tag="K0W59_002907"
     CDS             4656..5243
                     /locus_tag="K0W59_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HBI3023864.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGMKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(5305..6027)
                     /locus_tag="K0W59_002908"
     CDS             complement(5305..6027)
                     /locus_tag="K0W59_002908"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBI3023865.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(6029..7204)
                     /locus_tag="K0W59_002909"
     CDS             complement(6029..7204)
                     /locus_tag="K0W59_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBI3023866.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVLGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            complement(7231..8847)
                     /gene="bglH"
                     /locus_tag="K0W59_002910"
     CDS             complement(7231..8847)
                     /gene="bglH"
                     /locus_tag="K0W59_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HBI3023867.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVALENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(8933..10330)
                     /gene="bglB"
                     /locus_tag="K0W59_002911"
     CDS             complement(8933..10330)
                     /gene="bglB"
                     /locus_tag="K0W59_002911"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HBI3023868.1"
                     /translation="MTKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGK
                     MEPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEA
                     GLAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTR
                     YQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLI
                     PEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHN
                     ITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSER
                     GWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQ
                     VNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFG
                     WYAEVIKTRGLSLKK"
     gene            complement(10346..12223)
                     /gene="bglF"
                     /locus_tag="K0W59_002912"
                     /pseudo
     CDS             complement(10346..12223)
                     /gene="bglF"
                     /locus_tag="K0W59_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
     gene            complement(12357..13193)
                     /gene="bglG"
                     /locus_tag="K0W59_002913"
     CDS             complement(12357..13193)
                     /gene="bglG"
                     /locus_tag="K0W59_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HBI3023869.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMCLAINIERVRK
                     EH"
     gene            complement(13479..14204)
                     /gene="phoU"
                     /locus_tag="K0W59_002914"
     CDS             complement(13479..14204)
                     /gene="phoU"
                     /locus_tag="K0W59_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBI3023870.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGSDELDKLLAGKDSDK"
     gene            complement(14219..14992)
                     /gene="pstB"
                     /locus_tag="K0W59_002915"
     CDS             complement(14219..14992)
                     /gene="pstB"
                     /locus_tag="K0W59_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HBI3023871.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(15084..15974)
                     /gene="pstA"
                     /locus_tag="K0W59_002916"
     CDS             complement(15084..15974)
                     /gene="pstA"
                     /locus_tag="K0W59_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBI3023872.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(15974..16933)
                     /gene="pstC"
                     /locus_tag="K0W59_002917"
     CDS             complement(15974..16933)
                     /gene="pstC"
                     /locus_tag="K0W59_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HBI3023873.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(17020..18060)
                     /gene="pstS"
                     /locus_tag="K0W59_002918"
     CDS             complement(17020..18060)
                     /gene="pstS"
                     /locus_tag="K0W59_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBI3023874.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(18308..19381)
                     /locus_tag="K0W59_002919"
     CDS             complement(18308..19381)
                     /locus_tag="K0W59_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3023875.1"
                     /translation="MNKYIKQWCFSVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
BASE COUNT         5063 a         4926 c         4507 g         4924 t
ORIGIN      
        1 atttcaggaa ggagtgtccg ttaaactcag gctacctcag ggcgctggac aatgtgttac
       61 aggagctggg gcgcgaacgc acgattgcta tgagcctgcc ggaattcgag cagtcactgt
      121 ttatggcagc gcaacccgac aatctgctac tggcgaccgc gccgcgctac tgtcagtact
      181 acaatcaact ccatcaactg ccgttggttg ctcttcctct cccgtttgac gaaagccagc
      241 aaaaaaagct ggaagttcct tttaccctgc tgtggcataa acggaacagc cataatccga
      301 agatcgtctg gttacgggaa accattaaaa acctttacgc gtcgatggca taaccgaatc
      361 gtatgaaacg cgacccaatt tcacaataaa atgtaaaaaa gttgtaataa gcttgtctga
      421 atcgaacttt tagccgcttt agtctgtcca tcattccagt aaatgattac tcttgtattc
      481 ataatggacc attaagcatg gagcgaaaaa tggcgactca ctttgcccga gggattttaa
      541 cggaaggaca tctgatttct gttcgtctcc cctcccagtg tcatcaagaa gcccgaaaca
      601 ttccccctca tcgtcaaagc cgttttctgg cgtccagagg tttactcgca gaactgatgt
      661 tcatgctgta tggcattggc gaattgccgg aaatcgtcac cctgccgaaa ggtaaaccgg
      721 ttttcagtga taaaaatttg ccttcgtttt ctatttccta tgccgggaat atggttggcg
      781 tggcgttaac aaccgaaggt gaatgtggcc tcgatatgga actacagcgt gcgacgcgcg
      841 ggtttcatag cccacacgcg cccgataacc acaccttttc cagcaatgaa tcgctatgga
      901 tcagtaaaca aaacgatcct aacgaagcgc gggcgcagct cattacgctg cgccgaagcg
      961 tgctaaaact aaccggtgat gttttgaatg acgatccgcg cgatctgcag ctgctgccca
     1021 ttgccggacg cctgaaatgt gctcatgtaa atcatgtaga agcgttatgc gacgcggagg
     1081 acgtgctggt gtggtccgtg gcggtcacgc ccgcgattga aaagctcagt gtctgggagt
     1141 tagatggcaa acacagctgg aaaagcctgc cggatattca cagccgcgcc aacaatccta
     1201 ccagccggat gatgcgtttt gcccagctct ctaccgtgaa gtctttttcg ccaaattgat
     1261 agacaaccac aggagtcatt atgtctgaaa aattgcaggt ggttacgtta ctggggagcc
     1321 tgcgcaaagg ctcatttaat ggcatggttg cacgtaccct gccgaaaatt gctccggcga
     1381 gcatggaagt caatgcgtta ccttccattg ccgacattcc cctgtatgac gctgacgtac
     1441 agcaggaaga aggttttcca gcaacggttg aagctctggc ggaacagatc cgtcaggctg
     1501 acggtgtggt gatcgtcacg ccggaatata actactcggt accgggtggg ctgaaaaatg
     1561 ccatcgactg gctttcccgc ctgccggatc aaccgctggc cggtaaaccg gtgttgattc
     1621 agaccagctc aatgggcgtg attggcggcg cgcgctgtca gtatcacctg cgccagattc
     1681 tggttttcct cgatgcaatg gtgatgaaca agccggaatt tatgggcggc gtgattcaga
     1741 acaaagttga tccgcaaacc ggagaagtga ttgatcaggg tacgctggac cacctgaccg
     1801 ggcaattgac cgcatttggt gagtttattc agcgagttaa gatctaaata aaaaacccgc
     1861 cagtaatcat gcatggcggg tttttaatgc gctatcgatt ttaatgagcg tcgataaata
     1921 caatcttcag gataaacagc agcgcaacga tgattacgca cgggctaagg tcacgcagac
     1981 gcccggtacc aatcttcatc acgcagtagg agataaagcc cagcgcaata ccttcggtaa
     2041 tcgaaaagct gaacggcatc atcacggcgg taataaacgc cggaacagat tcagtaagat
     2101 cctgccagtt cacgcgagca agacttgagg tcatcaacac gccaacgtag atcaacgcgc
     2161 cagctgcagc gtagcctggc accattcccg ccagcggcga cagaaagata accagcagga
     2221 acagcagacc aacaaccact gccgtcagac cggtacgacc gccaaccgat acgccggaag
     2281 aggactcaat ataagccgta acggaagaag taccgataaa cgaaccggtc acggaagaga
     2341 tactgtcgac atacagcgcc tgcttcatgc gcgggaattt ccccttctca ttcgccagac
     2401 ctgctttatc ggtcacgcca atcagcgtac cggaggagtc aaacaagttg accaacatga
     2461 aagagaaaat cacccctgcc agcccgaggt taaacgaccc ggctaaatct acatgaccca
     2521 caactgtcat tacgctcggc ggcgcagaaa cgatgccatt gtagtgcaca tcacccagca
     2581 tccagcccag cagcgtcgtc accacgatag aaaccagcac cgctgcgtga atgttgcgcg
     2641 aggccagaat agcaatgatg aaaaagccga ggatacccag aagtacgctg tgagaagtca
     2701 gattaccgat gctcaccagc gtttccgagt tagcgacaat cacacctgcg tttttcagcc
     2761 ccatcatgcc gataaacagg ccgataccgc tggtaatacc cacacgcaga cttaccggaa
     2821 tgttagcaat catccagtaa cgaacgcgga aaatggtcaa aagaagcagt ccgacagcgc
     2881 cccagaagat tgcgcccata ccgatctgcc acggcagccc cattgcctgg acaaccacaa
     2941 aagcgaaaaa ggcgttaagc cccatcgcag gagccagcgc caccggaagg tttgcaaaca
     3001 atcccatcat aatgctaccg aaagcagcaa tcagacaggt tgttacgaag acggcactgg
     3061 tgtccatgcc agcaacgccc agaatttgtg ggttaacaaa tacgatgtag accatcgtca
     3121 ggaaggtggt gaacccggca atcacttcag ttcgtgccgt agtaccatgt tcgcgcagtt
     3181 taaaaacgcg ttccagcatc ctctgaccag aagtctgggt ggtatgttga tgactcatta
     3241 tctatttccg aacaaggagg gaaaatccgt cgctatcgta taccaaaatg cgacaatagg
     3301 cgcgtttgtg agagactttt tattggattt acttatacgg caacgattgc gttgcgcaaa
     3361 tcggctctac gaaaacgtta aactgattaa aaaggaaagg catgtcccgg atagaagcgg
     3421 tatttttcga ctgcgacggt acgctggtcg acagtgaagt catttgctcc cgcgcatatg
     3481 taacgatgtt tcaggaattt ggtattacgc tcgatcctga agaggtattc aaacgtttca
     3541 aaggtgtaaa actgtacgaa attatcgata ttgtttccct tgaacatggt gttacgttag
     3601 cgaaaacaga agctgaacac gtttaccgtg cagaagtcgc tcggctgttc gattcagaac
     3661 tggaagccat cgaaggggct ggagcgctcc tgtcagcgat cactgcgcca atgtgtgtgg
     3721 tatctaacgg cccaaataac aaaatgcagc attctatggg caagctgaat atgttgcact
     3781 acttcccgga taaactgttc agcggctacg atattcagcg ctggaagcca gacccggcgt
     3841 taatgttcca tgcggcaaaa gcgatgaatg taaatgtaga aaactgcatt ctggttgatg
     3901 actcagttgc cggtgcgcaa tctggtatcg acgcaggtat ggaagtgttc tacttctgcg
     3961 ccgacccgca caataagccg atcgttcacc cgaaagtcac cacctttacc catctttcgc
     4021 agttaccgga actgtggaaa gcgcgtggtt gggatattac ggcatagttc ttcacactcc
     4081 cttcacttac cccgcttaaa ttggcgctca aaggtaagta aagggagttt gatatgtctg
     4141 tttcacgtcg ggtaatacat cacggacttt attttgcggt tttaggaccg ttaattggtg
     4201 ttctgtttct tgtcctctac atattcttcg caaaagaacc gctggttctt ttggtaataa
     4261 tacaggtact tccattgttt ctcttattgt cgattactac gggagctatt cctgcattgt
     4321 taaccggcgt gatggtcgcc tgtctgccgg aaaagatcgg gtcacagaaa aattatcgtt
     4381 gtctggctgg tggcataggt ggcgtcgtta tcaccgagat ctattgtgca gttattgtac
     4441 atattaaggg catggcttcc tcggagttgt ttgaaaacat tctttctggt gacagtctcg
     4501 ttgtccgcat cattcctgca ttgctggcag gtgtggtgat gagcagaatc attacccgtc
     4561 tgcccggatt ggatatttca tgtcctgaaa cagactcttt aagttaagcg ggatacttta
     4621 tctttgggct actcaaaagc agacaggatg ttactatgac tcaaaatatc aggccgttac
     4681 cccaattcaa atatcatccc aagccactgg aaacaggcgc atttgaacag gataaaaccg
     4741 tagagtgcga ttgctgtgaa caacagacgt cagtttatta ctcgggtccc ttttattgcg
     4801 ttgatgaagt tgaacatctc tgtccgttgt gtattgcgga cggttctgct gctgaaaagt
     4861 ttgcaggtag ttttcaggat gatgccagca tagaaggtgt tgaatttgag tatgatgaag
     4921 aggacgaatt tgccggtatg aagaacacat atcctgatga aatgctgaaa gagttggttg
     4981 aacgcacgcc aggttatcat ggatggcaac aggaattctg gcttgcgcat tgtggcgatt
     5041 tctgtgcttt tatcggctat gtgggctgga atgatataaa agatcgcctc gatgaatttg
     5101 ccaaccttga agaagattgt gagaatttcg gtattagaaa ttctgatcta gctaaatgcc
     5161 tgcaaaaggg tggtgattgt cagggttatc tcttccgctg tctccactgc ggcaagctga
     5221 gactgtgggg tgatttttcg tagttattta aataatgaga acaggccgga gcgtaattca
     5281 tacatccggc cttatttctt cagcttaacc gaacgctaat tctgcggcag cggctttatc
     5341 agcgattacc atcagcgaag gatgcagctg caacacggaa gcaggaacat cttcggtaac
     5401 cggaccttgc agcacatttt tcagtgcctg cgctttacct gcgccactga caattatcag
     5461 caagtttttc gccgccatga tgcttttcgg ccccatcgtc acgtagctgt caggcactaa
     5521 cgaaaagtcg ccgcccaact caccatgcgc cacaatatca accatctctc cctggatcgg
     5581 gaactccacg gtttgctcat ggaaatgggt cgtattcggc aaattgccac aaaaatgacc
     5641 atctgcacct aatcccagca ccaccaaatc caggccgcct tcacgcgcca gtttctgatc
     5701 atgctcgcgg tagttatcaa tagtgagctt ctggatattc tcttctttga tccccgcagg
     5761 ggtgaaaaac agattacgca gattggtaat cgttacgcct tctccctctt tgccgcgaaa
     5821 gggaatttca tcgaaattat agaaatagca gttatcgtac caaggcttac ctttaaccag
     5881 ggtggtgagg tattcataca tgcctttggg cgtgctaccg gcggtaattg ccaggttaac
     5941 acgacgcgtc ttcgacatat aaccaagcag atggtgtgcc gcgacacggc tcatttcctg
     6001 gtaatcttcg gtaatgatta atttcatctt aatgccttat tttaaatttc tgtacgaagg
     6061 ctgcataggc aggccgccag acatccattt catggttcag gccgggatat tcctggtaat
     6121 caaacttaat ttttttctgc tcaagctcag ttttcagccc ggcgatatcc ttgccggtta
     6181 cgacatcttt atcaccaacg accacagtaa aattacgtag ttgctggtta atagctgccg
     6241 gatcgttcag ccgggccgcg acaccttcat ccggtacggt tgtcgtggta acaccactga
     6301 atgtggccag ccagccaaag ctttccagat gattcattcc ggaaaccagc gcctggtacc
     6361 cgccttgtga aagccctgcc agcgcgcgac catcggcatc tttacggaca ctaaaacgct
     6421 tgctaatcag cgggataata tcgttcatca gttcgcgatc tgccgcttta gcattcagcg
     6481 gataaaagac tttacgtctt tcctgaggca cgaaatcttc gggaataatg cccttcgcat
     6541 cggtttcggt atccgggatc accaccagca tcggtttaat tttcccttca gccagcaggt
     6601 tatccatgat ttgcgggata cgcccctgat cgatagcgga acgtccagta tcaccaaaac
     6661 cgtgatagaa atagagcact ggcaaaggct cgcccatgcc ggtgtatcct ggcggggtcc
     6721 agacatacat ctgacgttca gattgcaatg cgttggagtg gtaagttatg gcgatcaaat
     6781 caccatgcgc aacagaacgc gtatccagat aactgcctag caccagaatc atactggagt
     6841 taacctggcg ctgaggctta gtcattgcgg tgcctgtatc aatgctgcgt acaccatcaa
     6901 cattgaaaaa atactcgtac aggttgcctt tcaggatagg tgtgcgccac gaccagactc
     6961 ctgcttcgtc tttggtcatc gggtgaatat tgtccggaac cggaacaccc accactacag
     7021 agacattttt tgccccaggg gcaaagtagc gaaaggtcac gctgttatcg gcattgacct
     7081 gagtcacata ctgtttcacc gggatagtgg gtgccgggct ggctggcata tccgctgcta
     7141 tggcccactg agcagaaata ccgctggcaa tagccagcgt taaagcagca atttttatat
     7201 tcatcgtaat attcctttca ttaagttcca ttaccaccag atttcagcct gggcaccgac
     7261 agcaaactgg tcgtctttat tgtcttcgaa ggtgaagcca tccagttcgt tttccagggc
     7321 tttgatatac gtggcgtaga aacgaatttc cagacgcgag gtcaacatac tggtattgac
     7381 tttaaaggta tggaagagcg tggttttata accagactca ttaaatttat tactattcgc
     7441 atctttgttt tgctgggtga aatagcccag ttcaacacct gtctggttat attggtccca
     7501 aatataggcc gggcgaacaa ccgcacgaat agattcgaaa tcagagtgtg cgcctgtttc
     7561 gtagctataa atatcgttac cgaaggagta aacaatcgcg ttagccacaa taaaatgatc
     7621 gccgatatag gcttcgccct gcgaggtcag acgaactgct gttccgccgg tgtgatcacc
     7681 ataataacga ccattaaagg tggtaaatgg acttgcgcca gcataacggc caaagttcct
     7741 ggcgatagag ttattcgcaa ccaggaagga gaattcgttg aagccacctt tatcaaattt
     7801 ctgcgttaaa gatgtgccga acatccaggt atctttccag tcataatacc cgttattatc
     7861 ctgattatct ttttccgatg cgctttcgtt tgccgtgaca taacgaccac tcaccattaa
     7921 ggtcgcttta tcccataacg ggatatcttt atagcgtaaa tcaatagtat gggtattaat
     7981 ctgctgtttg ttttgcaggc tgcgatcgta atcatcgaca tcttcgcgaa ccagcgcgat
     8041 atcaatttta cccggaccga ctttccagtt ttccagagct acacccgctg cggcatcagt
     8101 acgctgcgtt ttccagtcca gcatctggat ttcaattttc ggcgcaccgt gtttacccac
     8161 ccagaaatca gcctctggcg caaagggcag gaaacctttg gtggtaacgt acatatcgga
     8221 gaactgcata aagttctcgc caccggcgtt atcgccaaac cagccggtag agtactgctg
     8281 accaacgtta ccatccatca tcacaacggc atcaacccgt ttgccgtttt cgttgtagac
     8341 acgttgtttt aactgcaaat caaaccagcc ggagtattcg ttaccaaagc ggcccagaga
     8401 accaatcgcc catgatttag gtgaaccatg agaggcggtc ccccaaccag aacggtaata
     8461 gccgttgtaa ctaaagccaa tttcatcttt cacaaactta ctgaaatctt tcagagtcat
     8521 ggaagaataa atcgagcctg tctcactggc atttttctct tcatttttga ccagtacagc
     8581 atcaggtttt gcggcactac tggtcgggaa cggattggcg gcataccctt gatcattcgt
     8641 acttacgcta cgattcaccg tcgctggcgt atattttttc ttctcttcat ctttatactt
     8701 tttgagttcg ctttgcgttt ctcttaacgc tttttctaat aactcaaggc gttgttccac
     8761 cgttaatgat tcagccaatg attgtgcaga aaaggctaac ggtgccatta ataagatggc
     8821 agaggtaata agatttcgtc taaacataat atccctttat ggtgcaaaga aagaattaac
     8881 gtatcgcatc cagactgttc tgaatgcgac gataattaag gtgctttatt ggttattttt
     8941 ttaatgacag cccccgcgtt ttgatcactt ctgcatacca tccgaagctt ttcttacgcg
     9001 ttcttgtgag gcttccttcg ccattatcat cacgatccac ataaataaag ccgtagcgct
     9061 tagacatttg tgaatgagat gcactgacta aatcaattgg cccccaactg gtgtacccca
     9121 taatatccac accatcggca atcgcttcat ttacctgtac caggtgatcg tttaaatagg
     9181 caattcgata atcatcctgt atcgaaccat ccgcttcaac gctgtctttt acgcctaatc
     9241 cattctcgac aataaataac ggtttttgat aacgatccca aagcgtattt aacagaaccc
     9301 gtaatccaac cggatcaatt tgccaccccc tctctgaact tttcagatgc ggattgggga
     9361 tcatattcag tatgttgccc tgcgcatttt tattaatgct ttcgtcgtgg gaaacacaac
     9421 cagtcatgta ataactaaaa gagatgaaat ctacagtatg ttttaaatct tctgcgtcac
     9481 tttcagtcat ctcaatggtg atattgtggt cgcggaagaa acgctgcata tagccgggat
     9541 actggccacg cgcctgaaca tcaccaaaga acatccagcg ccggttctct tccatggcct
     9601 gcaacatatc ctgtggctgg caggtgaggg ggtaaaccag cccaccgaga agcatattgc
     9661 cgattttcgc ttcggggatc aggctatgat aggctttaac tgcccgcgca ctggcaacca
     9721 gttgatgatg gatcgcctga taaacttccg cctcgccact ctcttctgcc agccccacgc
     9781 ccgtgaatgg cgcgtgtaac gacatattga tttcattaaa cgtcagccat aacgctactt
     9841 tatgttggta gcgggtaaag accgtgcggg cgtaatgctc gaagtgatcg atgaccgccc
     9901 gattagccca accgccgtag tttttcacca gcccatatag catttcgtaa tgggataacg
     9961 ttaccagcgg cttgatcccc gcctgcgcca tttcatcaaa cagccgatcg taaaacgcta
    10021 accccgcttc attcggttcg gcttcgtcgc cctgagggaa aattcgcgcc caggcaatgg
    10081 aaatacgcag acaggtgaag cccatctcgg caaataacgc gatatcttcc gggtaacggt
    10141 gataaaaatc gatggcgaca tctttgatat tctctttccc caggatgcgc ggttccattt
    10201 ttcccattac gccatgaggc tgtaaatctg aggtcgtgat ccctttgcca tcttccagcc
    10261 aggcaccttc cacctgattg gcagctgttg cgccacccca aagaaatgtt tctggaaatg
    10321 ctttcgtaat taactccttt tatcgttagc gaatgatgga taacagcggt tcacctgcgc
    10381 ttatctgcgc cgtgccgtgg ggtaatacgt ctgtaaaatc atcgctatta ctgattaata
    10441 ccggcgtcgt cagatcaaat ccggcctcgc gaatagcagg gatatcaaaa gaaatcagcc
    10501 gatcgcctgt attgaccttg tcacccacgt tgacgtgagc ggaaaagaat ttgccgtcca
    10561 gttttacggt gtcgataccg acatgaatca ggatctccac accatcatct gactcaatgc
    10621 caatggcgtg taatgtggcg aacaacgaag caattcgacc cgcaaccgga gaacgcactt
    10681 caccaaccga gggcagaatg gcgatacctt tacccagcag gccactggca aacgtggtat
    10741 cagcgacgtg aatgagcggc acaatctctc ccgtcatcgg tgaacagata ccgccctgct
    10801 caggtggtgt aataacctct ggtgttttct cttgcggggc accctgcgct ggctaacgtt
    10861 tactggtgat gaaatgaagc atcaacgtac caacaaatgc gcaacctatg gcaatgacac
    10921 cgccaataac gctggcccag acggtgaaat caatccccgt tgacgggatg gtctgcatga
    10981 aggtgaaaat acttggcaaa ccaaaggagt aaactttcgt ttgcgcgtag ccaatgatgg
    11041 tggcccccaa tgctccactg atacaggcga taacaaaggg gtacttacgc ggcaggttga
    11101 cgccatatac cgctggttcg gtgataccaa acagacccgt caacgccgct gatcccgcca
    11161 ccactttttt ctgcgcatcg cgttcgcaga ggaagacgcc gagcgccgcc ccgacctgcg
    11221 ccataatggc gggcattaac agcgggatca tggtgtcgta gcccagcacg gtgaagttat
    11281 tgatacacag cggcaccagg ccccagtgca gtccgaacat gacgaagatt tgccagaagc
    11341 cgcccattac cgcgcccgca aatgcaggaa ccgcctgata aagccagaga taaccggcgg
    11401 caatcatttc gcttatccag gttgatagcg gccccaccag cagaaaggtg acgggtgtga
    11461 taaccatcag acatagcaat ggtgtgaaga aatttttgat tgccgacggt aaccacgcat
    11521 taagtcggcg ttccagaatg ctgcacaacc aggcagaaaa aataatggga ataaccgatg
    11581 acgagtaatt caacaatgtg accggaatac ccaggaaatc cagccccggc gcatccgctt
    11641 tttgcccgtt ctcgaaggca gtcagaatta atggatgcac taacgctcca ccaatcacca
    11701 tggcagtaaa tggattaccg ccgaagcgtt tgcccgcggt gtatcccagg attatcggga
    11761 agaacccaaa caaggcatca ctggcgctga ataaaattaa ataagtacca ctttgttcgg
    11821 tcgtccactg aaaagtgagc gccagagcca gcataccttt caggatcccg gttgccgcca
    11881 tcagaccgat cagaggcgta aaaatacctg aaataacata aacaaagcgg tttagcagat
    11941 tacctttctc atcattttcc ggtgcctgtt gcgctttttc gccaaggcct gccacgctgt
    12001 taaccgccag gaagacatcg gccacatggt tacctatgac cacctgaaac tgaccaccac
    12061 tttccaccac cataataata ccgggggtct ttttcagtac ctcagcttgc gctttgcttt
    12121 catcctttaa cttaaaacgt aatcgcgttg cgcaatgcat cagactcaca atgttatctg
    12181 cgcccccgac tcctgcgact atttttctgg ctaactccgt cataacttgc cctctaccgc
    12241 tttgcggcaa aactccaaaa aaaaacctga aaaaaacggc ctgacgtgaa tcaagcaatt
    12301 tttttcaggt tttgcccgct tagtgcggta acaatccttt actcagtaat aatatttcag
    12361 tgttctttgc gcacgcgctc tatatttatg gctaaacaca taatctctgc gggtgaaatt
    12421 ttacgttgat actgtaaacc aataaaaatg gcgatccgtt ccgcacattg ccatgcttgc
    12481 gggtaatttt gttttactgc ttgttgtaat gattcatcac tatcgttaat tgaagcatgt
    12541 tcaagaatac gccaggataa aaacttcaga tgtgtaacca gtcgctgata actcaagctt
    12601 tcttcctggt aattaaggct gaactgaaat tttattaatt gcagcatttc gcgcattaac
    12661 tgcgtgacac ctgcaacatc ctccatattt ccgctcattt gggcactgac cagatgcatg
    12721 gcaataaagc ccacttcatc tttcggtaac tgcacgccca accgtttatc aataatggtt
    12781 aacgcttctt cccctagctg gaactctttc gggtaaagcc gctggatatc ccacagcaac
    12841 gggttgggca gcaacacgtt ttgctgaaag cgtttaatcg caaactggca atggtcagtt
    12901 agcgagatat aaatactgtc ctgtaatttt cccaagcgct cctgcgctaa agagataaga
    12961 cgatcacagg ttgccatcac ctcaagagga atatgactta agagttcgct taatcgcccg
    13021 ttcagttcat gactgctcaa ggcatactct ttttctattc cacttgagtt aattctttcg
    13081 ccagcgcgtt tttgaaagcc aattccgcgc cccatgacga ctttttcccg ctgttgatca
    13141 tcaataacca ccacaacatt attgttgaga attttggtga tttgcatgtt catagcaagg
    13201 acctttttta taaacaaaaa aacccgactt caccagtatt ctctggttat gtcaggtttt
    13261 gcctgcgaat gcagtaacaa tccagtcatt tattaatggt ttttataacg aacatccagg
    13321 ttcggaaatt aatttaattg cgtgcagaga aaatagcaat gcgctattga taaaaatatg
    13381 accatgctcg cagttattaa ctttgtgtaa ttttaggaat ttataaagtt atatataaca
    13441 aatcccaata attaagttat tgggatttgt ctggtgaatt atttgtcgct atcttttccc
    13501 gccagcagtt tatccagctc atcactgcct acatgacgga aatcctgacc cttcacgtag
    13561 tagaagataa actcacaaat attctggcag cggtcgccga tacgttcgat tgaacgcgca
    13621 cagaacagcg cggtaagtac gctcggaata gtgcgcgaat cttccatcat gtaggtcatc
    13681 agttgacgaa caataccttc gtattcctga tcgacttttt tatcttcacg ataaatacgt
    13741 accgcttcgt caatgtccat ccgcgcgaac gcgtccagca cgtcgtgcag catctggatg
    13801 gtatgacggc ccagcgactc cagacttacc agcaacggct gatgctgctg ggagaatttc
    13861 tccagcgcag tacggcagat tttgtccgcc acgtcgccaa tacgctccag ctcggcaatg
    13921 gttttactga tcaccataac cagtcgcagg tcgctcgccg tcggctgacg tttggcgata
    13981 atgcgcacgc aggcttcatc gatcgccact tccatcatgt tgacgttctt gtcgccttcg
    14041 atcacgcgct tcgccagatc gctgtcctgg ttatgcatcg cggtgattgc atcagaaagc
    14101 tgctgctcca ccatgccgcc catggtcatc acctgcgtgc ggatactttc cagttcggcg
    14161 ttgaactggc cggaaatatg tttattaaga ttgagactgt ccataacgca ctcctgaatc
    14221 aaccgtaacg accggtgatg tagtcttctg tttgtttctt cgctggcttg gtgaacagat
    14281 cgtccgtgtt gctgaactca atcaattcgc ccaggtacat aaatgccgtg tggtcggaac
    14341 aacgcgcagc ctgctgcatg ttgtgggtga cgatcaccac ggtgtaatcc tgcttcagtt
    14401 cggtgatcag ctcttcaata cgcccggtag agatggggtc gagcgccgaa cacggttcgt
    14461 cgagcagcag cacttccggg cgaatggcga taccacgcgc aatacacaga cgctgttgct
    14521 gaccaccaga gagagagtaa ccgctctggt gcaatttatc tttggtttcg ttccacaatg
    14581 cggctttggt caatgcccac tgcacgcgct cgtccatgtc ggcacgggag agcttctcaa
    14641 acagacgaac gccaaaggcg atgttgtcgt agatggacat cgggaacggc gtcggtttct
    14701 ggaacaccat gcccactttc gcacgcagca gggcgatatc ctgagagttg gtcaggatgt
    14761 tgtcgccatc aagcagaatt tcaccttccg cacgctgctc cgggtacagt tcaaacattt
    14821 tgttgaaggt acgcagcagc gtcgatttac cgcagccgga cggcccgata aacgccgtaa
    14881 cctggttttt ggcgatatcc aggttgatgt ttttcagggc atggaatttg ccgtagtaga
    14941 agttcaaatt acgaacctga attttactcg gggcagtttc aaccatactc attgcaatct
    15001 tttcctcatc gtacctgatg cttttcgcta atcaggctta ccgtagggcc gggtaaggcg
    15061 ttcacgccgc atccggcaaa gtgttaaccg tgtttattct tcgcaaaaac aacgcgcgcc
    15121 agaatgttca gcagcaggac gcacagggta atgatcaata ccccggccca ggccagttgt
    15181 tgccattccg caaacgggct catcgcaaac ttaaagatcg tcaccggcag gttggcgatc
    15241 ggctgcatca tgtccgtgct ccagaactgg ttggagagcg cggtaaacag cagcggcgcg
    15301 gtttcaccgg caatacgggc aatcgccagc aggataccgg tcataatccc ggacaccgac
    15361 gctttcagcg taatcgcgga gatcatcttc cacttcggtg tacccagcgc ataagccgct
    15421 tcacgcaggc tgtccggcac cagtttcagc atgttctcgg tggtgcggat aacgatcggc
    15481 acctgcaaca gcgccagcgc aattacgccc gcccagccgg agaagtgctc catctgtgcc
    15541 accacgatgg tgtagacaaa cagaccaacc acaatcgacg gcgcggagag cagaatgtcg
    15601 ttaatgaagc gaatcacttc tgccagccag gatttacgac catattccgc cagataaatc
    15661 cccgccataa tgcccagcgg cgtaccgaat accgtggccc acaaaattaa cagcccgcta
    15721 cccgccaggg cgttcgccag accaccacct tccgtattgg gcggcggcgt catttcagtg
    15781 aacagcgcca gcgacatacc gtcgataccg cgagtgatgg tggacattaa aatccagatc
    15841 agccagaaca ggccgaaggc catcgtcgcc atcgagagcg tcagcgcaat acggtttttg
    15901 aggcggcgac gcgcctgcat tttgcggcga gattcagcca gcgccgcagt ggtttgcatt
    15961 tcaaccatag ccattagcgt gccccctcat tcttagccag gcgcataatc ataaacttcg
    16021 atgcggcgag gacgatgaag gtaatcacaa acaggatcag gcccagttcc atcagtgcgg
    16081 caacgtgcag accggattcc gcttccgcaa attcgttcgc cagcgcagag gtgatactat
    16141 tgcccggcat gtacagcgag gcgctgtcga gctggtaggt gttaccgatg ataaaggtca
    16201 ccgccatggt ttcaccgagc gcgcggccca gccccagcat gatgccgccg ataacaccat
    16261 ttttggtgaa cggaagaacg atacgccaga taacttccca ggtggtgcag ccaataccgt
    16321 aggccgactc tttcatcatc accggggttt gttcgaacac atcacgcatt accgccgcaa
    16381 tgtacggaat aatcatgatg gcgaggatca cgcctgccgc gaggataccg ataccaaatg
    16441 cggggccaga gaacagcgcg ccaacaatcg ggatattcga catgatattg ccgaccggct
    16501 cctgaaagta aacggcgaac agcggcgcaa agataaacag gccccacatg ccgtaaacga
    16561 tacttggaat ggctgccagc agctcaatgg cgatacccag cgggcgtttc agccagccag
    16621 gcgcaagctc agtcaggaac agggcgatac cgaaactcac cgggacggcg atcagcagcg
    16681 cgataaacga agtcaccaac gtaccgtaga tcggcaccag cgccccgtag atatcgttcg
    16741 gtgcatccca ctctttggtc cataggaaag ccagaccaaa tttctgaatg ctcggccagg
    16801 aggagatgat cagagagaca ataatgccac ccaacatcaa tagcacaatc agcgccgcca
    16861 gttttaccag cacgctgaaa attatgtcgc cctttttacc cggtgggtta aaagcgggct
    16921 tggttgcagc cataagttac tcttcagtta aacgcgttta caaagtcatt ttgtaatgcc
    16981 ggatgcggcg taaaacaccg tacccggcct ggagttttat tagtacagcg gcttaccgct
    17041 actgtcttta atattggtct tccacgcagc gcgaacctgt tcaactacac tatccggcag
    17101 gctagcgtaa tccaggtcgt tcgcctgttt agccccggtt ttgtacgccc agtcgaagaa
    17161 tttcagcacc tctgtgcctt gttctggttt cttctgatct ttgtggatca gaatgaacgt
    17221 ggtagaggta ataggccatg catcttcgcc tttctggttg gtcagatcct gagcgaaggt
    17281 tttgctccag tctgcacctt ttgctgcatt agcgaagttt tcttcggtcg gactaaccgg
    17341 tttaccatca gcggagatca gtttggtgta cgccaggttg ttctgcttcg cgtaagcata
    17401 ttcaacataa ccaattgcac ccggcagacg ctgaacgaac gcggcgatac cgtcgttacc
    17461 tttaccgccc agaccgatcg gccattttac ggtagagcca gtaccaacgt tgtttttcca
    17521 ctcttcgttc actttcgcca ggtagctggt gaagacgaag gaagtcccgg agccatctgc
    17581 gcggcgtact acagcaatgt tttgtgaagg cagtttcaga cccggattca gtttggcgat
    17641 ggcttcatca tcccacttct tgattttgcc taggtagatg tcgccgaggg ttttaccatc
    17701 cagcaccagt tcgccagact tcagccctgg aatgttaacc gccagcacca cgccgccaat
    17761 cacggtcggg aactggaaca gaccttcctg agccagtttt tcgtcagaca gcggcgcgtc
    17821 agaggcacca aaatcaacgg tattagcgat aatctgtttt acgccaccgg aagaaccgat
    17881 accctggtag ttaactttat taccggtttc tttctggtaa gtatcagccc atttggcata
    17941 caccggcgca gggaaggttg cacctgcacc tgtcaggctt gcttctgcaa acacagagaa
    18001 agcactcatc gataaggtcg cggcgacaac agttgcgacg gtggtacgca taactttcat
    18061 aatgtctcct gggaggagtc ataaagcatt gtttgttggc tacgagaagc aaaataggac
    18121 aaacaggtga cagttatata taaggaatat gacattttta tgacagaaag gaattaccaa
    18181 ataacttact gattttaaat gtgaagatga aaatcccaca tgccatcttt ttgtcatgat
    18241 gcatttaaaa gtgataaaga aatgggtgag atgacaacga tgtgatgaac aatcgttgtc
    18301 agacctatta gcgcacaatc tcaacagtta tcgtcgctgt tgcagtaaat tgccccgggg
    18361 ctgggcgagc tccagtaagg ctcgcaggag cagctgaaaa agttacacta cctgattttt
    18421 caccatacac tgttgattta tccatgtctg ttggcaactc accaccgttc actttgattt
    18481 ccttattatt tttgtcgtaa atttttatcc ccacgttaga gtttgaagtt acgaccattg
    18541 aattgtccgt attgggttcg gtagcataaa aagacgtact gatatcatcg gtattcagta
    18601 tccctaggtt ttcacactca atagtgactg ttttagtaat tttatgtgtt gttaccgcac
    18661 ttccaacaac agatgaaaaa tctgcagctg gtatttcttt aaaatcaaaa caaactggct
    18721 ttaaattatt tatttcgcaa ctttgcggag ctgtaatatc gccccgaata taaacatcag
    18781 ccatcggctg tgagtaatcc accggggtgt cacggctaat tgttccatac aatgtcacta
    18841 tctttgtttc aggaataatc aattttcctg caaatgtctt cttgatataa aaatatacta
    18901 tcgctttagc cccactggat atacttaaag gttcaatgcg gttgcaattg tactttgtat
    18961 taccagacgg gttgttagca tgatattcaa aaggtgcatt cacataccca accccaagaa
    19021 tatctatagc aatcccaaca tcaaggttcg gattaagatc ataataatta atattagagt
    19081 gaaccccagc ataagttaat gcctggttta tttttgacgt ataatagata taggggaatt
    19141 ctcctacgtt accagtatca caatgacagc gtccaagata tgaggatcca ttagttacag
    19201 tggtgttaaa tgtcgttcca accttatttt cgttcgcggt aataagatat cccgagaaat
    19261 caagcgtact gtgaaatacg ccattatcag gggtacagat acctttagga gctgcaagtg
    19321 ctacactact taacgagagc ataaacacag aaaagcacca ctgtttgata tacttgttca
    19381 taaaaatcct tttaatgaca tgtcacaccc tgttcaatta
//
LOCUS       DAETPZ010000074        18261 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.074, whole genome shotgun sequence.
ACCESSION   DAETPZ010000074 DAETPZ010000000
VERSION     DAETPZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.074"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(62..820)
                     /gene="aroD"
                     /locus_tag="K0W59_002920"
     CDS             complement(62..820)
                     /gene="aroD"
                     /locus_tag="K0W59_002920"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBI3023876.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="K0W59_002921"
     CDS             complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="K0W59_002921"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HBI3023877.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1729..3000)
                     /locus_tag="K0W59_002922"
     CDS             complement(1729..3000)
                     /locus_tag="K0W59_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3023878.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3183..4387)
                     /locus_tag="K0W59_002923"
                     /pseudo
     CDS             complement(3183..4387)
                     /locus_tag="K0W59_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(4487..4843)
                     /locus_tag="K0W59_002924"
                     /pseudo
     CDS             complement(4487..4843)
                     /locus_tag="K0W59_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            complement(4979..5086)
                     /gene="rprA"
                     /locus_tag="K0W59_002925"
     ncRNA           complement(4979..5086)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="K0W59_002925"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="K0W59_002926"
     CDS             complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="K0W59_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HBI3023879.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6773..9829
                     /locus_tag="K0W59_002927"
     CDS             6773..9829
                     /locus_tag="K0W59_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBI3023880.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9826..10236
                     /gene="menI"
                     /locus_tag="K0W59_002928"
     CDS             9826..10236
                     /gene="menI"
                     /locus_tag="K0W59_002928"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HBI3023881.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10336..10524
                     /locus_tag="K0W59_002929"
     CDS             10336..10524
                     /locus_tag="K0W59_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HBI3023882.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11072..11440
                     /gene="sufA"
                     /locus_tag="K0W59_002930"
     CDS             11072..11440
                     /gene="sufA"
                     /locus_tag="K0W59_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HBI3023883.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEIDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11449..12936
                     /gene="sufB"
                     /locus_tag="K0W59_002931"
     CDS             11449..12936
                     /gene="sufB"
                     /locus_tag="K0W59_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBI3023884.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12946..13692
                     /gene="sufC"
                     /locus_tag="K0W59_002932"
     CDS             12946..13692
                     /gene="sufC"
                     /locus_tag="K0W59_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBI3023885.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETHDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13667..14938
                     /gene="sufD"
                     /locus_tag="K0W59_002933"
     CDS             13667..14938
                     /gene="sufD"
                     /locus_tag="K0W59_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBI3023886.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTVHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGFKQQVLARIAQRLPGGAR"
     gene            14935..16155
                     /gene="sufS"
                     /locus_tag="K0W59_002934"
     CDS             14935..16155
                     /gene="sufS"
                     /locus_tag="K0W59_002934"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HBI3023887.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALGCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16168..16584
                     /gene="sufE"
                     /locus_tag="K0W59_002935"
     CDS             16168..16584
                     /gene="sufE"
                     /locus_tag="K0W59_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HBI3023888.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16733..17737
                     /gene="ldtE"
                     /locus_tag="K0W59_002936"
     CDS             16733..17737
                     /gene="ldtE"
                     /locus_tag="K0W59_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HBI3023889.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="K0W59_002937"
     CDS             complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="K0W59_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HBI3023890.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4804 a         4405 c         4690 g         4362 t
ORIGIN      
        1 acggttaaaa atactttttt tatttcggcg ggggagggta tttccgccga aatattattg
       61 cttatgcctg atgtaaaata gttaataccg tgcgcaaatc atttaccgag atttgccctg
      121 gcgcagaggc tttttttacc gcaccaaaag ttgccgccga gccaaatact tcaccagcca
      181 gacgagaaat tacgccagtt tttgccatcg acatcgtgat gattggacga tcggcatact
      241 gctcttgcat ctccagggtc gcggcaagca acgtcagcac atcgctggta ctttgcggca
      301 tcagcgcaat cttaggaata tcggcgtcga aggactgcat tttgcgcaga cgggcaatga
      361 tttcttcggc ttccggcgtt ttatggaagt catggttgga catgacaact ttcacatcat
      421 gcgcgtgggc gtaggcgacg gtttctttga cctgatcatc gccggtaaat aactccagat
      481 cgatcatatc aaccaggccg ctgtcgatgg ctgcacgatt gagagcaata taagcctcgg
      541 tggaaatcgc ctgctcgccg ccttctttgg cactgcggaa ggtaaacagc agcggttttt
      601 ctggcatggt ttcacggaga atttttgccg ccgccatgac agactccaca ttggagaggt
      661 cggcaaagtg gtccacacgc cattccagaa tatcaaagtc cgcttcacga taggcgagag
      721 cttcggattt cacgcgggcg atatctttcg ccatcagcga gacgatgatt ttaggtgcgc
      781 ccgcaccaat gacgagatct tttacagtta cggttttcat tttttaccct ttctgcacgc
      841 ggtcagcctg tcaggcaccg aaccccatga cctgtttaac atattccaga gggaaatctt
      901 tgccagtcca taacgtgaac tgttcagccc cttgccacaa caacatgccg tatccatcaa
      961 tcgttttgca accagcttgt tgtgcctgct gcaataactt cgtcatatgc gggttataca
     1021 cgcattcagt gaccagcagt cccggatgta acagactgat atcattaacc aatgattcat
     1081 tctcaagggg tttcatacct acttttgtgc cattggttaa aatgtcggcg gaagccaggg
     1141 cttcagcaaa ggcttgctga tcggcgagat cggtaaccgt gacgacacaa tcggtgtttt
     1201 cgttaacccg ctgcgcgaag gcgagggctt tatcgaagaa ctcatcccga cggttaaaga
     1261 gtttaatttc ttttaaacct tcaattgccc cctgcgcgcc aatcgccgtt gaggcacctc
     1321 cggcccctaa cagcaccatc gttttgcctt tgatatcaaa accgctctct ttaatggcgc
     1381 gaatatggcc cgtgccgtcg gtgttatagc cacgcagata gccatcatca ttaacgatgg
     1441 tgttgatggc acccaccagt ttggctgctg gcgttaattc atcaacatat tcacacgcca
     1501 gttgtttgtt cggcatcgac acaccagttc cgcgcatttt gagggctttt aatccttcaa
     1561 ttgctccggg aaagctatcg ttatccactt cgaaggccat ataggtaaat ggcaatcccg
     1621 ctttttctaa ggctttattc tgcatttcag gcgataaact gtggcggata ggataagcca
     1681 tcaatccaat caattcgtat tttgcggtaa catccataat taactccttt aacctctatg
     1741 cttaattgtg tttaaccggg tgctaaattt gcgttcgcca aaccgcacat cattctcagg
     1801 aataataaaa acgcggtaat agcggctaaa aacaataatt gcggtaatca gggccagcag
     1861 cgcgaaagta aaatcgagaa caatgatata ttgcaggccg atgttcgaca gataaccggt
     1921 aatcagcgga ataacaaagt tagccagtcc acccatcatc atataaatac tggtgacttt
     1981 ggctttgctt ttggggaaaa actctgacat gaccgaaaca ccgagctgta aaatgccgcc
     2041 agctgctgag aaaccgataa caaaggcgcc ggcattacac accagtggag aagggtacag
     2101 gtagataatg gcagctgtta ttgttgccag cgcagagtta aatacattag cccagatggg
     2161 ccggaccatt tttttcagta gtgcggcaaa aataaagaca cagaccaacg agcccatact
     2221 gtaataagag atggttttta atgcctcagc ttctgacata ccagcaaaag ccatcgcata
     2281 tttgggcatc cacaccacaa tcacataaaa ggtcgagaat gcggctacac cgaacagtac
     2341 cgatgaaaca ccttccagcc agactaacgg tttgctgttc atttgcggta attcattagc
     2401 tacgctggcg tccaccaact ggctggggaa tttgcttttc aacagcatca gcgtgatcag
     2461 tacaaacaga atacccggaa taatcagccc atagccgtac cagatattat tgagcaacat
     2521 atagctcacc agcattgggt agaacatttg cccaaatgac accatcgctt taaccagtat
     2581 gaccgccgaa ccagaggctt tcggaaagca ttccatgagc gcggggtagc cacccgtatc
     2641 cagcgctgag ttagcgatac ctacgcacac tgccagaccg taggcgagag ttaaattcgg
     2701 gcaagcggga ataccaaaga agaatagcag atacattatt actgccatta atatcaccgc
     2761 ccgacgacca aacttatcgg agatcacacc gaagaataaa atactgatca atcgccccaa
     2821 accgataccg gaaattaaat aggcaatgcc cgcgttgtca gtggaaaact tttctgccag
     2881 agatgtcata ttttgggcaa gcgtaataac actaatgccg tgcaggaaat agctgaagta
     2941 aatacaaaga acagccagga taaatggcgt gctgaaagat aatttcgtta ccgtacgtat
     3001 tgttctccga cggtttacat aattactata ctgaagatga ttttccctgg gtacatggca
     3061 taaagtttat gtttccagga atattgcatg tcatagtttg tttttgaacg cgattttcct
     3121 tagttagcgc agaaagagtt atttatccgt aagatcttgc atgacctgaa aagaatattg
     3181 cattacccac ccggagcgac attttccttt agcgagtgat gccgcgtttt tttgcggctg
     3241 cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag tatcgaacca cataatatcg
     3301 gcaatacttc tttgggacag atgagccgta atcaacggaa tagtaaaggt cgcaatactg
     3361 cccgcactgt aatagatccc tgtagcttta cctttagcgt aagggaaacg ttcagctatt
     3421 aacgtcaggc caatttgcac aacacctcca gcagaggtaa aaccaatgac aaaagcaaat
     3481 attatcacca cataaaatgt gggatgcagg cagacggtaa acagagcaat aaatgagata
     3541 aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat tacgaatgag tggagcggta
     3601 ataaatacac aaagcagcga acccacggtg tagatactga gtagtttgat cgacatagtg
     3661 tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc actggctaac cagataaaac
     3721 gttgccattg agatatagcc atataaggta taactggcta aatcgataat tgaacagcga
     3781 tgttccgtag agctggtggt tttctttatg acaggtaagc gacgacccgg atggggtggg
     3841 aacgtacaac ggtataaaaa cagagcgtta ataaacataa tgcctgcagc aatcataaag
     3901 gaccaaccga accacagttc agcccacact aacaggctaa tgattagcgg taataaaaat
     3961 tgtccgctgg aaacaaatgc tttaattaaa atattggctg tcccaggtga gcgtggaaaa
     4021 gcttccatca aactgggata agtgcctgca tcgagaaaac tgtttgccat tcccgccaga
     4081 aagccaaaaa cataagcgat aatgatgtta ttggtctgca ggatgccaaa aaagaaggcc
     4141 atatagcagc acattccgag catgataaaa gggcggcgac caaagcgatc ggataataat
     4201 cctgcaaaaa gcaagacact taatcgacca atgcccagcg atgagataac tatcgagaca
     4261 cccgcggcat tagtctgaca aagtgtctcc agcgaggcca tattcaggct catcaaaatg
     4321 acgcccatac catgcaccag gtaataaaat acaacccaag tgcggtaggg taatagggat
     4381 ttttcatagg ttctacttag caaaggtgct ttaaaaagtg tagcgatgtt gtgtcagtat
     4441 ccattcatta ttgttgtaac aacgacaatt cctttctgtg gaaaaattaa caaagccgct
     4501 ccagatcgtg tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat
     4561 tgccatcagg gtagacttgt tgaaacgcgg ttaagtcggg gaaaaaacgc atggtgttgt
     4621 tgccgatgcg gttatttatt ttctctatga tggcatgaag atgttttttt cgttactgac
     4681 gcatagccaa cagttgctcg actacacaat caggaatggc gcatttgcca ttttcccact
     4741 gacgccagct ttcactgtca ccggtctggg caatccaggt cgcgcattca tcaatagtca
     4801 tggcaaaaat atggcggagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg
     4861 aaataaacaa taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca
     4921 gtatcttgtg caacattatt ggaaagagtg aggggcgagg tagcgaagcg gaaaaatgtt
     4981 aaaaaaaagc ccatcgtggg agatgggcaa agactacaca cagcaattcg ttgtttcact
     5041 caggggattt ccatgcttat aaatcaatat gttgatttat aaccgtgagc taatagtagg
     5101 catacggact ttttgcgtcg atcagattcg tctcattagt ttaaaaactg taaagaattt
     5161 gcgacaggta ctaccagctg aatggcggta agcaggaaaa tatggggaag agcaagcaaa
     5221 tatcaattag cggtgattaa caatcaggcg gcagagccgc ctgatgagaa attacttatt
     5281 cggtttctcg atttcgccaa gttcttcgag aatttcttcc ggttggtccg tcggcggcgg
     5341 gacttcttcc acccacgcgg caaacagacg ccaggaaacg gctaacagaa ccggaccaat
     5401 aaacagaccg atcatcccga aagcaatcaa accaccaata acgccagaga ggatcaggat
     5461 cagcggtaaa tcggcaccca tgcgaattaa cattgggcgg atgacgttat ccagcgtgcc
     5521 aaccacaccg ctccacacta acaataccgt tccccaggtg gtatcgccgg tccagtagag
     5581 ccagataatc gccggaatca gtaccggcaa cgggccaagc tggacaaggc aggagaggat
     5641 cattaacacc gttagcagcg ttgcataagg tacgccggat acggcgaggc cgataccgcc
     5701 aagcactgcc tgtactaacg ccgtcaccac cacacccagc gccaccgcgc ggatagcctg
     5761 tgccgccagc agcacggcgg catctccgcg aacgcctgcc agacgggttg caaaatggcg
     5821 aatgccttgt gccacctgtt caccgcgcca gtacagcagg gcactgaaga gaagcatcag
     5881 cgcacaatgc accataaagc gcccgatatg cgccgcctgc ccaacgaacc aggtggtggt
     5941 ggtgccaata taagggcgga ctttcgccat gatcgccgta ccccccatat ccagcaagtt
     6001 gtgccagcct gcatacagct tcgcgccaat caccggaatg gtattaagcc acgccagatc
     6061 gggtaacgtc atgtcgccgc tggaaatggc tttaattagc gggccgctgc cgtcgacgat
     6121 actgttaacc agcaaagcaa tagggatgat aaacaccatc actaataaca gcgtcatcac
     6181 cagaacggcg agagagcggc ggccaaacat gatcttttgc aaacgtaaca ataccggcca
     6241 ggtggcgata accaccgtac cggcccatgc aaagccgaga ataaagggtt gaacaatcca
     6301 cagacatgcc acaatcatga tggctaaaaa cagcaccgaa agcagaattt gtgcgacatc
     6361 cctgggctga cgaacattta ccataactac ttttcacctt ttttgtgcgt caaatcgtcg
     6421 acgcgacgtg gacacgcgaa actcacctgc ataatgatga gcaatttcag cggtttttaa
     6481 caggccgatt ctgtccttaa ttctgatggg cgtgttagaa aaaaatgtgg tacaacaact
     6541 caggcaacac gcaaacgatt tactcgtcgt atttcaagtt gcatgtgctg cggctacgtt
     6601 cgttcacccc agtcacttac tattgtaagc tcctggagtt cactcgcttg ccgccttcct
     6661 gcaactcgaa atccatagag taaataaaca tcacctttca gtgcgctatt gcagccgcct
     6721 gaaagaatag tgtatcgacc tacatcacag accgcaggaa agggtcaata taatgattcc
     6781 acagatttcc caggcacccg gcgtcgttca actggtgctt aattttttgc aagagctgga
     6841 gcaacaaggt tttaccggcg atgcggcgac aagttatgcc gatcgtctga caatgtcgac
     6901 cgacaacagt atttaccaac ttctccccga tgcggtggta tttccgcgtt caaccgcaga
     6961 tgtggcgctg atcgcccgtc ttgccgcgca ggaacgctat tcatcgctga tctttacccc
     7021 ccgcggcggc ggcaccggca ctaacggtca ggcgctcaac caggggatta ttgttgatat
     7081 gtcccgccat atgaaccgca tcatcgaaat taaccctgaa gagggctggg tgcgcgttga
     7141 ggccggggtg ataaaagacc aactcaatca gtacctgaaa ccgttcggct acttttttgc
     7201 gccggaactt tcgaccagca accgggcaac gctcggtggg atgatcaata ccgatgcatc
     7261 cggtcaggga tcgctggtct atggcaaaac gtcagatcac gtacttggcg tacgcgcggt
     7321 gttgttgggg ggcgatattc tcgatacgca acctttaccc gtcgagctgg cggaaacgct
     7381 gggtaaatcc aataccacaa tcgggcgaat ttataacacg gtttatcaac gttgccgtca
     7441 gcaacgccag ttaatcatcg acaacttccc caaacttaac cgctttctta ccggttacga
     7501 tctgcgtcat gtctttaacg atgagatgac cgagttcgac ctgacgcgca ttctgacggg
     7561 ttcagaaggg acgctggcct ttattaccga agcgcggctg gatattacgc gcttgcctaa
     7621 agtgcgccgt ctggtgaatg tcaaatatga ctcttttgac tctgcgctgc gtaacgcgcc
     7681 gtttatggtt gaggcgcggg cgctttcggt agagacggtg gactcaaaag tgctgaatct
     7741 ggcgcgggaa gatattgtct ggcattccgt cagcgagttg attaccgatg tgcctgacaa
     7801 agagatgctc ggactgaaca ttgtggaatt tgctggtgat gatgaggcgc tgattgacga
     7861 gcgagttaat gcactctgtg tgcggcttga tgagctgatt gctagccaac aggcaggtgt
     7921 gattggctgg caggtgtgcc gcgagctggc gggggttgaa cgtatctatg cgatgcgcaa
     7981 aaaagccgtt ggtctgcttg gcaatgccaa aggtgccgct aagccaattc cgtttgctga
     8041 ggatacctgc gtaccgccgg aacatctggc cgattatatt gctgaatttc gcgcgctgct
     8101 cgacagccac ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg tcttgcacgt
     8161 ccgtccggca ctggatatgt gcgatccgca acaagagatt ttgatgaagc aaatctctga
     8221 tgacgtggtg gcgctgactg cgaaatacgg tggtttgttg tggggcgagc acggcaaagg
     8281 ttttcgcgct gaatacagcc cggcgttttt cggtgaggaa ctttttgcag aactgcgcaa
     8341 agtgaaagcg gcatttgacc cgcataaccg actcaaccca gggaagattt gcccgccaga
     8401 aggcctcgat gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat tcgatcggca
     8461 gatccccatt gcggtacgcc agcagtggcg cggtgcgatg gagtgtaacg gcaacggttt
     8521 atgcttcaac tttgatgccc gtagtccgat gtgtccgtcg atgaagatca cccagaaccg
     8581 gattcattca ccgaaagggc gcgcaacgct ggtgcgtgaa tggctgcgct tgttggcgga
     8641 tcgcggcgtt gacccactca aactggaaca agaactgcct gaatccggcg tcagtttgcg
     8701 gacgttaatt gcccgcacgc gcaatagctg gcatgcgaat aaaggcgaat atgacttctc
     8761 acacgaagtc aaagaggcga tgtcgggctg tctggcctgt aaagcttgtt cgacccagtg
     8821 ccccatcaaa attgatgtgc cggagtttcg ctctcgtttt ctgcagctct atcacacccg
     8881 ttatttacgc ccgcttcgcg accacctcgt tgctacggtc gagagctacg caccgctgat
     8941 ggcacgcgcg ccgaaaacct ttaacttctt cattaaccag ccgctggtgc gcaaactctc
     9001 ggaaaaacat atcggcatgg ttgatttgcc actgctgtcg gtcccctcgc tacaacaaca
     9061 aatggtgggg catcgctcgg caaacatgac gctggaacag cttgaagctc tcaatgcaga
     9121 gcagaaagcg cgcacggtgt tggtggtgca ggaccccttt accagctatt acgatgcgca
     9181 agtggtggcg gattttgtcc gtctggttga aaaattaggt ttccagcctg tgttactgcc
     9241 attttcgcca aatggcaaag cccagcatat caaaggcttt cttaatcgtt ttgcgaagac
     9301 ggcgaaaaag acggcggatt tcctcaaccg tatggctaag cttgggatgc caatggtggg
     9361 cgtcgatccg gcgctggtac tttgttatcg cgatgaatat aaactggcgc tgggcgagga
     9421 acgtggcgag tttaacgtct tacttgcgaa tgaatggctg gcaagcgcac ttgagtcaca
     9481 tccagtggct acagtcagcg gtgaatcatg gtatttcttt ggtcactgta ccgaggttac
     9541 cgctttgccg ggcgcgccag cacaatgggc cgcgatattt gcccgttttg gcgcgaaact
     9601 ggaaaatgtc agcgtgggtt gctgcggcat ggcagggact tacggacatg aagcgaaaaa
     9661 ccatgaaaat tcgctcggga tctatgagtt atcctggcat caggctatgc agcgactgcc
     9721 gcgtaaccgc tgtctggcga ccggatattc ctgccgtagc caggtaaaac gggttgaagg
     9781 cacgggggta cgccatcctg tgcaggcttt actggagatt attaaatgat atggaaacgg
     9841 aaaatcaccc tggaagcact gaatgctatg ggtgaaggaa acatggtggg gttcctggat
     9901 attcgctttg aacatattgg tgatgacacc cttgaagcga caatgccagt agactcgcgg
     9961 acaaagcagc ctttcgggtt gctgcatgga ggagcatccg tggtactggc cgaaagtatc
    10021 ggttccgttg ccggttattt atgtaccgaa ggtgagcaaa aagtggttgg tctggaaatc
    10081 aatgctaatc acgtccgctc ggcacgagaa gggcgggtgc gcggcatatg taaaccgttg
    10141 catctcggtt cgcgtcacca ggtctggcag attgaaatct tcgatgagaa agggcgtttg
    10201 tgctgttcgt cacgattgac gaccgccatt ttgtgacgtt gtcaccagaa aagtgtgacg
    10261 catattgctt ttggtcatta tctgcacagt aaacggtggt atactgtgca ggtcttgtct
    10321 aaaagcgagg atgctatgtc tactcaactt gatcccaccc agctggccat tgaattttta
    10381 cgccgtgacc agagcaatct ttcacctgcc caatatctca aacgtctgaa acaactagaa
    10441 cttgaatttg ctgacctgct tactctctct tctgctgaat taaaagaaga aatctacttt
    10501 gcctggcgtc tgggcgtgca ttgatcctga cttaatgccg caagttcgcc aaatcgacct
    10561 ggattaacct ccgtccaggt tcgatgacct tcttaatact gcttatctac cgcctgatat
    10621 tgttcaaaaa taatactaat cgcagtttgt gttaaaacgg cgggttagct ttatgagatt
    10681 attcttatcg ccccttcaag agctaagcca ctgagagtgc cggagataag cgccggatgg
    10741 ggtagaaacc cttaagcctg tgtcgcacag acttaagggt tttcttattt ctgaacgctg
    10801 cactttttta cctatctctt aaatagtcat tttcaataca actttttctt tttcatcaca
    10861 gccttaatgc tccttagata ataaccatta tctaacaatg agatacctaa ttcttagcgt
    10921 gcctgttaac ccacacatca ggggctatgc ttattaaaag caggcaaaaa gcatgtttta
    10981 cggtaaagcc cctgcgtttg ctgggttgaa ctgataatca ttatcactaa catgctgtta
    11041 tacgctgaaa gcgatgaagt gaggtaaata gatggacatg cattcaggaa cctttaaccc
    11101 acaggatttc gcctggcaag gcttaacgct gacacccgca gcggcgatac acatccgcga
    11161 gctggtggca aagcagccag gtatggtcgg cgtgcgctta ggtgtgaagc aaacgggctg
    11221 cgcgggcttt ggctatgtgc tcgacagtgt tagcgagccg gacaaagacg atctgctgtt
    11281 tgaacacgac ggcgcgaagc tgtttgtccc gctgcaatcg atgccgttta ttgatggcac
    11341 ggaaatcgat ttcgttcgtg aaggacttaa tcagatattc aaatttcaca accctaaagc
    11401 ccagaatgaa tgtggctgtg gcgaaagctt tagggtatag gcggtactat gtctcgtaat
    11461 actgaagcaa ctgacgatgt caaaacctgg accggcggcc cgctgaatta taaagaagga
    11521 ttcttcaccc agttagccac cgatgagctg gcaaagggga taaacgaaga ggtggtgcgc
    11581 gcaatttcgg cgaagcgtaa tgagccggag tggatgctgg agtttcgtct aaacgcctat
    11641 cgcgcatggc tggagatgga agaaccgcac tggttgaaag cgcactacga caagctgaat
    11701 tatcaggatt acagctacta ctcagcacca tcgtgcggta attgtgacga cacttgcgcg
    11761 tctgaacctg gcgcggtgca gcaaactggc gcgaacgcct ttttaagtaa agaggtggag
    11821 gcggcgtttg agcagttggg cgttcccgtg cgggaaggca aagaggtggc ggtggatgcc
    11881 attttcgact ctgtttcggt tgccaccact tatcgcgaaa aactggcgga gcagggaatt
    11941 attttctgtt cctttggtga ggcgatccac gatcacccgg aactggtgcg taaatatctc
    12001 ggcaccgtgg tgccggggaa tgacaacttc tttgcagcac ttaatgcggc ggtagcctct
    12061 gacggtacgt ttatttatgt gcctaaaggc gtgcgctgcc cgatggaact ttccacctat
    12121 tttcgcatta acgcggaaaa aaccgggcag tttgagcgca caattctggt ggccgacgaa
    12181 gacagctacg tcagctacat tgaaggctgt tccgctccgg tgcgtgacag ctatcagttg
    12241 cacgcggcag tggtggaagt catcatccat aaaaacgccg aggtgaaata ttccacggta
    12301 caaaactggt tccctggcga taacaacacc ggcggtattc tcaacttcgt caccaagcgt
    12361 gctttgtgcg aaggcgaaaa cagcaaaatg tcatggacgc aatcagaaac cgggtcagcg
    12421 attacgtgga aatatcccag ctgcattttg cgcggcgata actccattgg tgagttttac
    12481 tcagtggcgc tgaccagcgg tcatcagcaa gcggataccg gcaccaagat gatccacatc
    12541 ggtaaaaaca ccaaatcgac cattatctcg aaagggatct ctgccggaca tagtcagaac
    12601 agttatcgcg gcttagtgaa aatcatgccg acggcaacca atgcgcgcaa tttcactcag
    12661 tgcgactcaa tgctgattgg cgctaattgt ggggcgcata ccttcccgta tgtcgaatgt
    12721 cgcaataaca gcgcacaact ggagcacgag gcaacgacat cacgtattgg tgaagatcaa
    12781 ctgttttact gcctgcaacg cgggatcagc gaagaagacg ccatctcgat gattgttaac
    12841 ggtttctgca aagacgtgtt ctcagaactg ccgctggaat ttgccgttga agcacaaaaa
    12901 ctcctcgcca tcagtcttga acacagcgtc ggataaggaa taaacatgtt aagtattaaa
    12961 gatttacacg tcagcgtgga agataaagct atcctgcgtg gattaagcct cgacgttcgt
    13021 cccggcgaag ttcacgccat tatggggcca aacggttcgg gcaaaagtac tttatcggca
    13081 acgcttgccg ggcgcgaaga ttatgaagtg acgggcggca cggttgagtt caaaggcaaa
    13141 gatttgcttg cactgtcgcc ggaagatcgt gcaggcgaag gcatctttat ggccttccag
    13201 tatccggtgg agattccagg cgtcagtaac cagtttttcc tgcaaacggc acttaatgca
    13261 gtgcgcagtt atcgcggcca ggaaacgcac gaccgctttg attttcagga tttgatggaa
    13321 gagaaaatcg ctctcctgaa gatgccggaa gatttattaa cccgttcggt aaacgttggt
    13381 ttttccggcg gcgagaaaaa gcgcaacgat attttgcaaa tggcggtgct ggaaccggag
    13441 ttatgcattc ttgatgagtc ggactccggg ctggatattg acgcattaaa agtggtcgcc
    13501 gatggcgtga actcgctgcg tgatggcaag cgctcattca tcattgttac gcactaccaa
    13561 cgcattctcg actacatcaa gcctgattac gttcatgtgc tatatcaggg acgaattgtg
    13621 aaatccggcg atttcacgtt ggtcaaacaa ctggaggagc agggttatgg ctggcttacc
    13681 gaacagcagt aatgcgctgc aacagtggca tcacttgttt gaagctgaag ggacaaaacg
    13741 ctcaccgcaa gcacagcagc atttacaaca attgctgcgt accggactgc cgacacgtaa
    13801 acatgaaaac tggaaatata cgccgctgga agggctgacc aatagtcagt ttgtcagcat
    13861 tgcgggagat atatccccac agcagcgtga tgccttagcg ttaacgttag acgccgtgcg
    13921 gctggtgttt gtcgatggac gttacgtgcc cgcgctgagc gatgcaattg aaggcagcgg
    13981 atatgaagtg agcattaacg acgaccgtca ggggttaccc gacgctattc aggcggaagt
    14041 gtttctgcat ttgacggaaa gcctggcaca aagcgtgacg catatcgccg tgaagcgcgg
    14101 tcaacggccg gcaaagccat tgctgttaat gcatatcacc cagggcgtgg caggtgaaga
    14161 ggtgaacact gtccattacc ggcatcatct ggatctggcg gaaggtgccg aagcaacggt
    14221 gatcgaacat tttgtcagcc ttaatgatgc tcgtcatttt accggggcac ggttcactat
    14281 caatgtcgca gcgaacgccc acttgcagca tatcaagctg gcgtttgaaa acccggtcag
    14341 tcaccacttt gcccataacg atttgttgct ggctgacgat gccaccgcat ttagccatag
    14401 tttcctgctg ggtggcgcag tgttacgaca caacaccagt acgcaactca atggcgaaaa
    14461 cagcacgctg cggatcaata gcctggcgat gccggtgaaa aacgaggtgt gtgatacccg
    14521 tacctggctg gaacacaata aaggtttttg taacagccga cagttgcaca aaactatcgt
    14581 cagcgacaaa ggccgcgcgg tatttaacgg tttgatcaac gtcgcgcagc acgccatcaa
    14641 aacggatggt cagatgacca acaataatct gctgatgggc aaactggcgg aagtggatac
    14701 aaaaccgcag ctggaaatct atgcagatga tgtgaaatgc agccacggcg cgacggtggg
    14761 gcgtattgat gatgaacaga tgttctatct gcgctcgcgc gggatcaatc agcaggatgc
    14821 ccagcagatg atcatttacg cctttgctgc cgaactgacg gaagcactgc gtgatgaggg
    14881 gtttaaacag caggtgctgg cccgaatcgc tcaacggctg ccaggaggtg caagatgact
    14941 ttttccgtcg acaaagtgcg ggccgacttt ccggtgcttt cgcgtgaggt aaacggtttg
    15001 ccgctggctt atctcgacag cgccgccagt gcacagaaac cgagccaggt gattgacgcc
    15061 gaggccgagt tttatcgtca tggctacgcg gcggtgcatc gcggtattca taccttaagc
    15121 gcccaggcga ccgagaaaat ggagaacgtg cgcaagcggg catcgctgtt tattaatgcc
    15181 cgttcggcgg aagagctggt gttcgtccgc ggcacgacgg aagggatcaa tctggtcgcc
    15241 aatagctggg gcaacagcaa tgtgcgggcg ggcgataaca tcatcatcag tcagatggag
    15301 caccacgcta acattgtccc ctggcagatg ctttgcgcac gcgttggcgc agagctgcgt
    15361 gtgatccctc tcaaccccga cggtacgctg caactggaga cgctgcctac gctgtttgat
    15421 gagaaaactc gcctgctggc aattactcat gtctccaacg tgcttggcac agaaaatcca
    15481 ctggcggaaa tgatcacgct tgcgcaccag catggcgcaa aagtgctggt ggatggcgct
    15541 caggcggtga tgcatcatcc ggtggatgtt caggcgctgg gttgcgactt ttacgtgttc
    15601 tccgggcata aactgtatgg ccccaccgga attggcattc tttatgtgaa agaagccttg
    15661 ttgcaggaga tgccgccgtg ggaagggggc ggttctatga tcgccaccgt cagcctgagt
    15721 gaaggcacta cctggaccaa agcaccatgg cggtttgaag ccggtacacc caataccggg
    15781 ggcatcattg gtcttggcgc ggcgctggag tatgtttcgg cgctggggct taatagcata
    15841 gccgagtatg aacagaatct gatgcattat gcgctatcac agctggaatc tgtaccggat
    15901 ctcactctct atggccaaca aaacaggctt ggcgttattg cttttaatct cggtaaacac
    15961 cacgcctatg atgttggcag ttttctcgat aattacggca ttgctgtgcg taccggacat
    16021 cactgcgcta tgccattaat ggcctattac aacgtccctg cgatgtgtcg ggcgtcgctg
    16081 gccatgtata acacccatga agaagtggat cgtctggtga ccggcctgca gcgtattcac
    16141 cgtctgctgg gataacaggg aggcactatg gctttattgc cggataaaga aaaattgctg
    16201 cgtaattttt tacgctgcgc caactgggaa gagaaatatc tctacattat tgagctgggc
    16261 cagcgtctgc cagaattacg cgccgaagac ggaagcccac aaaatagcat tcagggctgc
    16321 cagagtcagg tgtggattgt catgcgccag aatgcgcagg gaattattga attgcagggc
    16381 gacagcgatg cggcgattgt gaaagggctt attgcggtcg tctttattct ctacgatcag
    16441 atgacgccgc aggatattgt caatttcgat gtgcgtccgt ggtttgaaaa aatggcgctc
    16501 acccaacatc tcaccccatc tcgttcacaa ggtctggaag cgatgattcg cgcaattcgc
    16561 gccaaagccg ctgcacttag ctaaactaaa gggacagctt tcatccggtt ggctcacatt
    16621 gccggatggt caatgtttac actgacctgc acaggagtgt tttggtttca ggtgaacata
    16681 agcaccatcc gacgtttcag cgtgagtctc cggcaaatac aggaggttta caatgaaacg
    16741 cgcgtctttg cttacactca cgcttatcgg cgcttttagc gccatccagg ctgcctgggc
    16801 ggttgattat ccgctaccac caaccggaag ccgactggtt gggcaaaatc aaacgtatac
    16861 ggtgcaagaa ggggataaaa accttcaggc tattgcccga cgttttgata ctgcggcaat
    16921 gttgatcctt gaagccaata acactatcgc cccggtgcca aaacctggta cgacgataac
    16981 tattccttca caactgttat tacctgatgc accgcgtcag gggattatcg ttaaccttgc
    17041 agagctgcgc ctttattatt atccgccggg agaaaatatt gtgcaggttt atccaatagg
    17101 tattggattg caggggctgg aaacgccggt gatggaaacg cgtgttgggc agaaaatccc
    17161 taacccaacc tggacgccta cggcaggcat tcgtcagcgt tcgctggagc gtggcattaa
    17221 attaccgcca gtcgttcctg ccggaccaaa taacccgcta ggacgttacg cactgcgcct
    17281 cgcgcatggt aatggcgaat acctcattca tggcaccagt gcgccggaca gcgttggttt
    17341 gcgcgtcagt tcagggtgta ttcgcatgaa tgcaccggat attaaagcct tgttctccag
    17401 cgtgcggacg ggaacgccgg tgaaagtgat caacgaaccg gtgaaatatt ccgtggaacc
    17461 taacgggatg cgttatgttg aagtacatcg accactatca gcagaagaac agcaaaacgt
    17521 tcagacaatg ccatacacac tgccagcagg ctttacgcaa tttaaagaca ataaggctgt
    17581 agatcagaag ttagtcgata aagcgttgta tcgtcgggca gggtatccgg tttcggtgag
    17641 cagtggagca actcccgcag ccagcaatgc gccttcagta gagtcagcgc agaatggtga
    17701 accagagcaa gggaatatgt tacgcgcgac gcagtagtgg taaatagcag acaaaaaaaa
    17761 tggcgcacaa tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag
    17821 ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg
    17881 aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt
    17941 cagaagacag ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca
    18001 ggattaccgc gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt
    18061 taatctccat gtagcgttac aagtattaca caaagttttt tatgttgaga atattttttt
    18121 gatgggaagg cacttatttt tgatcgttcg ctcaaagaag catcgaacgc tgggttttag
    18181 ataaaaaaat tgagagaaaa caggatgctt ccatcggatt catcttaaat aaagtgagat
    18241 tatatagtga aatccgattt g
//
LOCUS       DAETPZ010000075        18173 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.075, whole genome shotgun sequence.
ACCESSION   DAETPZ010000075 DAETPZ010000000
VERSION     DAETPZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18173
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.075"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..2083
                     /gene="pbpC"
                     /locus_tag="K0W59_002938"
                     /pseudo
     CDS             1..2083
                     /gene="pbpC"
                     /locus_tag="K0W59_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            2233..2664
                     /gene="ndk"
                     /locus_tag="K0W59_002939"
     CDS             2233..2664
                     /gene="ndk"
                     /locus_tag="K0W59_002939"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBI3023891.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2814..3968
                     /gene="trmG/rlmN"
                     /locus_tag="K0W59_002940"
     CDS             2814..3968
                     /gene="trmG/rlmN"
                     /locus_tag="K0W59_002940"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HBI3023892.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4253..5266
                     /gene="rodZ"
                     /locus_tag="K0W59_002941"
     CDS             4253..5266
                     /gene="rodZ"
                     /locus_tag="K0W59_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HBI3023893.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5293..6411
                     /gene="ispG"
                     /locus_tag="K0W59_002942"
                     /gene_synonym="gcpE"
     CDS             5293..6411
                     /gene="ispG"
                     /locus_tag="K0W59_002942"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBI3023894.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            6522..7796
                     /gene="hisS"
                     /locus_tag="K0W59_002943"
     CDS             6522..7796
                     /gene="hisS"
                     /locus_tag="K0W59_002943"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBI3023895.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7814..8434
                     /gene="yfgM"
                     /locus_tag="K0W59_002944"
     CDS             7814..8434
                     /gene="yfgM"
                     /locus_tag="K0W59_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HBI3023896.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8445..9623
                     /gene="bamB"
                     /locus_tag="K0W59_002945"
     CDS             8445..9623
                     /gene="bamB"
                     /locus_tag="K0W59_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HBI3023897.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTTVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9741..11213
                     /gene="der"
                     /locus_tag="K0W59_002946"
     CDS             9741..11213
                     /gene="der"
                     /locus_tag="K0W59_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBI3023898.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11283..11498
                     /locus_tag="K0W59_002947"
     CDS             11283..11498
                     /locus_tag="K0W59_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBI3023899.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11495..12865)
                     /gene="xseA"
                     /locus_tag="K0W59_002948"
     CDS             complement(11495..12865)
                     /gene="xseA"
                     /locus_tag="K0W59_002948"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBI3023900.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13027..14493
                     /gene="guaB"
                     /locus_tag="K0W59_002949"
     CDS             13027..14493
                     /gene="guaB"
                     /locus_tag="K0W59_002949"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBI3023901.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14562..16139
                     /gene="guaA"
                     /locus_tag="K0W59_002950"
     CDS             14562..16139
                     /gene="guaA"
                     /locus_tag="K0W59_002950"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBI3023902.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEAQKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKTDLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16234..16773)
                     /locus_tag="K0W59_002951"
     CDS             complement(16234..16773)
                     /locus_tag="K0W59_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HBI3023903.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16789..17307)
                     /locus_tag="K0W59_002952"
     CDS             complement(16789..17307)
                     /locus_tag="K0W59_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBI3023904.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17618..17809)
                     /locus_tag="K0W59_002953"
     CDS             complement(17618..17809)
                     /locus_tag="K0W59_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HBI3023905.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWLHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17827..17979
                     /locus_tag="K0W59_002954"
     CDS             17827..17979
                     /locus_tag="K0W59_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023906.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
BASE COUNT         4299 a         4451 c         4994 g         4429 t
ORIGIN      
        1 gaagcgctga tcaattatga agatcgctgg ttctggaagc atccgggggt taatccattc
       61 tcggtggcgc gcgcagcatg gcaagatctc acttcgggac gggttatttc cggtggcagc
      121 acgctcacta tgcaggttgc tcgtctgctt gatcctcacc ccaaaacatt tggtggcaaa
      181 attctccagc tctggcgcgc gttgcaactg gagtggcatt tgtctaagcg tgaaattctg
      241 atcttgtatc ttaaccgcgc tccgtttggc ggtacgttgc aggggatcgg tacggcaagt
      301 tgggcttatc tcggaaaatc gcctgcgaat ttaagctatt ccgaggcggc aatgctggcg
      361 gtgttaccgc aagtgctcag ccgactgcgc ccggatcgtt ggccggagcg tgccgaagcc
      421 gcgcgtaata aagtgctcga acggatggct gcgcaaggtg tgtggtcccg tgagcaggta
      481 aaagagtcaa gggaagaacc catctggctg gcaccccgac aaatgccgca actggcaccg
      541 ctgttttcgc gcatgatgct cggtaaaagc aaaagcgaca aaatcgttac tacgctggat
      601 gccggtcttc aacgacgtct ggaagaactg gcgcaaaact ggaaagggcg gttgccaccg
      661 cgcagctcac tggcgatgat cgtggttaat cataccgata tgcgtgttcg cggctgggtg
      721 ggatcggttg atctcaacga tgattcacgc tttggtcatg ttgatatggt caatgcgatc
      781 cgatcgccag gatcggtgct caaaccgttt gtttatggtc tggcgctgga tgaaggcttg
      841 atccacccgg catcactgct gcaagacgtc ccccggcgca ccggtgatta tcgaccaggt
      901 aactttgata gcggttttca tggcccgatc aacatgagcg aggcgctggt gcgctcgctg
      961 aacttaccag ctgtgcaggt gctggaagcc tatggaccga aacggtttgc ggcaaagtta
     1021 cgcaatgttg gattgccgtt atatttgccc aacggcgctg cgccgaatct ttcactcatt
     1081 ctcggcggcg ctggtgcaaa actggaagat atggcggcag cgtataccgc gtttgctcgc
     1141 cacggcaagg caggcaaatt gcgcttacag cctgacgatc cgctgcttga acggccttta
     1201 atgtcgtcgg gggcggcgtg gatcattcgc cggattatgg ctgatgaagc gcaacccttg
     1261 ccggatagtg ccttgccgcg cgtcgcccca ctggcatgga aaacgggcac cagctatggc
     1321 tatcgtgacg cctgggcgat tggggttaac gctcgctatg tcattgggat ctggactggc
     1381 agacctgacg gcacgcccgt tgttggtcag tttggctttg ccagtgccgt accattagtt
     1441 gaatcaggtc aataatatct tactgtcgcg cagtgcgaat ctgccggaag acccgcgccc
     1501 gaactcagtc actcgtggcg ttatctgctg gccgggcgga cagtctttgc cggaaggtga
     1561 cggtaactgt cgccgccgcc tggcaacctg gctgctggat ggcagccagc cgccaacgct
     1621 gttactgtca gagcaggaag ggattaatgg cattcgcttc cccatctggc tggatgacaa
     1681 agggcagcgc gtcgccgctg acttcccgca ggcgaagcaa gaaatggtaa atgtctggcc
     1741 gctaccgctg gaaccctggc tgcccgcatc agaacgtcgc gctgtgcgct taccaccggc
     1801 ctcgacaatt tgcccgccat acggtcacga tgcccagctc cctctgcagc tgacaggcgt
     1861 tcgcgatggc gcgattatta aacgtttacc gggcgcagcg gaagcgactt tgccgttgca
     1921 atcaagtgga ggggcaggtg aacgctggtg gtttctgaat ggcgaaccgt taactgaacg
     1981 cgggcgcaac gtgaccctgc atttgacgga taaaggtgat tatcaattgc tggtgatgga
     2041 tgatgtgggg caaatcgcga cagtgaaatt tgtcatgcaa tagtctgttt tgtccctgat
     2101 tgttgctaaa actcatttta ttttaaaaaa aatgttacct tcatcaatag tcaacggccc
     2161 tgttgctcat tataatccgc gccatctcgt acgctggtac agacaacaac agaacaattt
     2221 acagaggtaa aaatggctat tgaacgtact ttttccatca tcaaaccgaa cgcggtagca
     2281 aaaaacgtca ttggtaatat ctttgcgcgc tttgaagctg cagggttcaa aattgtcggc
     2341 accaaaatgc tgcacctgac cgttgaacag gcacgtggct tttatgctga acacgatgga
     2401 aaaccgttct ttgatggtct ggttgaattc atgacctctg gcccgatcgt ggtttccgtg
     2461 ctggaaggtg aaaacgccgt tcagcgtcac cgcgatctgc tgggcgcgac caatccggca
     2521 aacgcactgg ctggtactct gcgcgctgat tacgctgaca gcctgaccga aaacggtacc
     2581 cacggttctg attccgtcga atctgccgct cgcgaaatcg cttatttctt cggcgaaggc
     2641 gaagtgtgcc cgcgcacccg ttaataattt cgtaaatgcc gcgtgcaaac gtggcatccg
     2701 tgcgccagaa tttgtacaat gtagcgcccc cggacgagca gccgctcacc ggggcgtttc
     2761 ttttttcaac cctccagggg ccataacgtg taataacgag gccggaatag catatgtctg
     2821 aacaattagt cacacctgaa aacgtcacca cgaaagatgg aaaaatcaac ctgctggatc
     2881 tcaaccgtca gcagatgcgg gagtttttta aagatttagg tgaaaaaacc ttccgcgccg
     2941 atcaggtgat gaagtggatg tatcactatt gctgcgacaa ctttgatgag atgaccgaca
     3001 tcaacaaagt gttgcgcggc aaactgaaag aggtggcgga aatccgcgca ccggaagtgg
     3061 ttgaagaaca gcgttcatcc gacggcacca ttaaatgggc gatcgccgtt ggcgatcagc
     3121 gcgtcgaaac ggtgtatatc ccggaagacg accgtgccac gctctgcgtc tcttcgcagg
     3181 tggggtgtgc gctggagtgt aaattctgtt ccaccgccca gcagggcttt aaccgcaacc
     3241 tgcgggtatc ggaaattatt ggccaggtgt ggcgtgcggc gaaaatcgtc ggcgcagcaa
     3301 aagtcaccgg tcagcgccca atcactaacg tcgtgatgat gggcatgggc gagccgctgc
     3361 tcaacctgaa caacgtcgtt ccggcgatgg aaatcatgct tgatgatttc ggttttggcc
     3421 tgtctaaacg tcgcgtgacg ctttccactt ccggtgtagt tccggcgctg gataaactgg
     3481 gcgatatgat cgacgttgca ctggcaattt ccctgcacgc gccgaacgac gaaattcgtg
     3541 acgaaattgt gccgatcaac aaaaagtaca atatcgaaac gttccttgct gcggtgcgcc
     3601 gttatctgga gaaatccaac gccaatcagg gccgagtcac tattgaatac gtgatgcttg
     3661 accacgttaa cgacggcact gaacacgcgc accaactggc ggaactgctg aaagatacgc
     3721 cgtgtaagat caacctgatc ccgtggaacc cgttcccggg cgcgccgtat ggacgcagtt
     3781 cgaacagccg tatcgaccgc ttctcaaaag tgctgatgag ctacggtttc accactattg
     3841 tgcgtaaaac gcgtggtgat gatatcgatg ctgcctgtgg tcagttggcg ggcgatgtta
     3901 tcgaccgtac gaaacgtact ctgcgtaaac ggatgcaggg tgaagccatc gatattaaag
     3961 cggtctgata gccattacgc cacggttact ttatgtaccg tggcgtaatc ttttatgaat
     4021 gaagtattaa gttgcaattg tctggtcaat attaacggtg cgtttttgct gactttaagg
     4081 cagtatgtaa cgatgcaaca gtaagttagc ctcagtgaat gtgggctttg tcacgagcac
     4141 acagacggtc ttatactgta tgataacggt taacttaacg gatgtttcgc ggtgtgggtg
     4201 agcatgatgg ttcaccggca tctcaattct catttaaacg tacctgcagc gaatgaatac
     4261 tgaagccacg cacgaccaaa atgaagcact tactaccggc gctcgcctgc gtaatgctcg
     4321 cgaacaacta ggacttagtc agcaggccgt tgccgagcga ctttgcctga aggtttccac
     4381 ggtacgcgac attgaagaag ataaggcacc cgccgatctt gcttcaacat tcctgcgcgg
     4441 atatatccgc tcttatgcgc gtctggtaca tattccagaa gaagaactgc tgccagggct
     4501 ggaaaagcag gctccacttc gggctgcaaa agttgcgcca atgcagagtt tttccctcgg
     4561 taaacgccgc aaaaaacgcg acggctggct gatgaccttc acctggctgg tgttgtttgt
     4621 ggttatcggc ctgagcggtg cctggtggtg gcaagaccac aaagctcagc aggaagagat
     4681 caccactatg gccgatcaat cttcggcgga actgagcagt aatagcgagc aggggcagag
     4741 tgttccgtta aatacgtcga caactacaga cccggctaca acgtccacgc cgccagcgtc
     4801 tgtggatact accgcaacca acacgcaaac acctgccgta actgcgccag caccagctgt
     4861 tgatccgcaa cagaatgcgg ttgtttcgcc ttcgcaggca aatgttgata ccgccgcgac
     4921 cccggtacca acggcagcaa caacgccaga tggtgctgcg ccgttgccaa ccgatcaggc
     4981 tggcgtgacc acgccggcgg ctgatccgaa tgcgctggtg atgaacttta ctgccgattg
     5041 ctggctggag gtcactgatg ctaccggtaa aaaattgttt agcgggatgc agcgtaaaga
     5101 cggtaatttg aatttaaccg gccaggcacc gtataaactg aaaattggtg cgccagccgc
     5161 agtacagatc cagtatcaag ggaaacctgt cgatctgagt cgttttatca gaactaacca
     5221 ggttgcgcgt ctgaccctca atgccgaaca atcaccggcg cagtaacaga cgggtaacgc
     5281 gggagatttt tcatgcataa ccaggctcca attcaacgta gaaaatcaac acgtatttac
     5341 gttgggaatg tgccgattgg cgatggtgct cccatcgccg tacagtccat gaccaatacg
     5401 cgtacgacag atgtcgaagc aacggtcaat caaatcaagg cgctggaacg cgttggcgct
     5461 gatatcgtcc gtgtctccgt accgacgatg gacgcggcag aagcgttcaa actcatcaaa
     5521 cagcgggtta acgtgccgct ggtggctgac atccacttcg actatcgcat tgcgctgaaa
     5581 gtagcggaat acggcgtcga ttgtctgcgt attaaccctg gtaatatcgg taatgaagag
     5641 cgtattcgca tggtggttga ctgtgcgcgc gataaaaaca ttccgatccg tattggcgtt
     5701 aacgccggat cgctggaaaa agatctgcaa gaaaagtatg gcgaaccgac gccgcaggcg
     5761 ttgctggaat ccgccatgcg tcatgttgat catctcgatc gcctgaactt cgatcagttc
     5821 aaagtcagcg tgaaagcgtc tgacgtcttc ctcgctgttg agtcttatcg tttgctggca
     5881 aaacagatcg atcagccgct gcatctgggg atcaccgaag ccggtggtgc acgcagcggg
     5941 gcagtaaaat ccgccattgg tttaggtctg ctgctgtcgg aaggcatcgg cgacacgctg
     6001 cgcgtatcgc tggcggccga tccggtcgaa gagatcaaag tcggtttcga tattttgaaa
     6061 tcgctgcgta tccgttcgcg agggattaac ttcatcgcct gcccgacctg ttcgcgtcag
     6121 gaatttgatg ttatcggtac ggttaacgcg ctggagcaac gcctggaaga tatcatcact
     6181 ccgatggacg tttcgattat cggctgcgtg gtgaatggcc caggtgaggc gctggtttct
     6241 acactcggcg tcaccggcgg caacaagaaa agcggcctct atgaagatgg cgtgcgcaaa
     6301 gaccgtctgg acaacaacga tatgatcgac cagctggaag cacgcattcg tgcgaaagcc
     6361 agtcagctgg acgaagcgcg tcgaattgac gttcagcagg ttgaaaaata ataacgtgat
     6421 gggaagcgcc tcgcttcccg tgtatgattg aacccgcatg gctcccgaaa cattgaggga
     6481 agcgttgagg gttcattttt atattcagaa agagaataaa cgtggcaaaa aacattcaag
     6541 ccattcgcgg catgaacgat tacctgcctg gcgaaacggc catctggcag cgcattgaag
     6601 gcacactgaa aaacgtgctc ggcagctacg gttacagtga aatccgcttg ccgattgtag
     6661 agcagacccc gctattcaaa cgtgcgattg gtgaagtcac cgacgtggtt gaaaaagaga
     6721 tgtacacctt tgaggatcgc aatggcgaca gcctgactct gcgccctgaa gggacggcgg
     6781 gctgtgtacg cgccggcatc gagcatggtc ttctgtacaa tcaggaacag cgtctgtggt
     6841 atatcgggcc gatgttccgt cacgagcgtc cgcagaaagg gcgttatcgt cagttccatc
     6901 agttgggctg cgaagttttc ggtctgcaag gtccggatat cgacgctgaa ctgattatgc
     6961 tcactgcccg ctggtggcgt gcgctgggta tcttcgaaca cgtaactctt gagctgaatt
     7021 ctatcggttc gctggaagca cgcgccaatt accgcgatgc gctggtggca ttccttgagc
     7081 agcataaaga aaagctggac gaagactgca aacgccgcat gtacactaac ccgctgcgcg
     7141 tgctggattc aaaaaatccg gaagtgcagg cgcttctcaa cgacgctccg gcattaggtg
     7201 attatctgga cgaggaatct cgtgagcatt ttgccggtct gtgcaaactg ctggagagcg
     7261 cggggatcgc ttacaccgta aaccagcgtc tggtgcgtgg tctggattac tataaccgta
     7321 ccgttttcga gtgggtgact aacagtctcg gctcccaggg caccgtgtgt gcaggcggtc
     7381 gttatgacgg tcttgtggaa caactgggcg gtcgtgcaac accggctgtc ggttttgcga
     7441 tgggcctcga acgtcttgta ttgttagtac aggccgttaa tccggaattt aaagccgatc
     7501 ctgttgtcga tatatacctg gtggcttcag gtgctgatac acaatctgcg gctatggcat
     7561 tagctgagcg tctgcgtgat gaattactgg gcgtgaaatt gatgaccaac cacggcggcg
     7621 gcaactttaa gaaacagttt gcccgtgctg ataaatgggg tgcccgcgtt gctgtggtgc
     7681 tgggtgagtc tgaagtggct aacggcacag cagtagtgaa ggatttgcgc tctggtgagc
     7741 aaacggcagt tgcgcaggat agcgtagccg cgcatttgcg cacgttactg ggttaaggaa
     7801 ggagaaggac agcgtggaaa tttacgagaa cgaaaacgac caggtagaag cggttaaacg
     7861 cttttttgct gaaaatggca aagcactggc tgttggggtg attttgggcg ttggcgcact
     7921 gattggctgg cgctactgga acagccatca ggttgattct gcacgctccg cttctcttgc
     7981 ctatcaaaat gcggttaccg cagtgagcga aggcaaaccg gatagcatcc cggcggcgga
     8041 aaaatttgct gctgaaaata aaaatactta tggtgcgctg gcttctttgg aacttgcgca
     8101 gcaatttgtt gacaaaaatg aactagagaa agctgccgcc cagttacaac aggggctggc
     8161 agacacgagc gatgaaaatc tcaaagccgt gataaatctg cgtcttgctc gcgttcaggt
     8221 acagctcaag caggctgatg ccgcgctgaa aacccttgat accatcaaag gtgaagggtg
     8281 ggctgccatt gttgccgacc tgcgtggtga agcattgctg agcaaaggtg ataagcaagg
     8341 tgcgcgtagt gcatgggaag caggcgtgaa aagcgatgtt actccggcac tgagcgaaat
     8401 gatgcagatg aaaattaata atttgtccat ctgagaggga cccgatgcaa ttgcgtaaat
     8461 tactgctgcc aggactgctt tccgttaccc ttttaagcgg ctgttcgctg tttaacagcg
     8521 aagaagatgt ggtaaagatg tccccattgc caaccgttga aaaccagttt acgccgacca
     8581 cggcgtggag cacttccgtt ggtagcggca ttggcaactt ctattccaat cttcatccgg
     8641 cactggcgga caacgttgtc tatgcagcgg accgcgctgg tttggtaaaa gcgctgaatg
     8701 cggatgatgg caaagaaatc tggtctgtca gcctggccga gaaagatggc tggttctcta
     8761 aagagcctgc attactttct ggcggtgtga ccgtgtctgg tgggcatgtc tacattggca
     8821 gcgaaaaggc gcaggtttac gcgctgaata ccagcgatgg tactgtggca tggcaaacta
     8881 cagtcgcggg tgaagcactt tcgcgcccgg tggtcagcga cggtctggtg ttaatccaca
     8941 ccagtaacgg tcagttacaa gcgctgaacg aagctgacgg cgctgtcaaa tggacagtta
     9001 acctcgatat gccttcgctc tctttgcgtg gcgagtctgc gccggcaacg gcttttggtg
     9061 cggccgtcgt gggtggcgat aatggtcgcg tcagcgcagt gctgatggaa cagggccaga
     9121 tgatttggca gcagcgtatt tcccaggcga ccggttctac cgaaattgac cgtctgagcg
     9181 atgttgacac gactcccgtc gttgttaacg gcgttgtttt cgcgctggcc tataatggta
     9241 acctgacggc gcttgatctg cgcagtggtc agattatgtg gaaacgcgaa ctgggttcgg
     9301 tgaatgattt catcgtcgat ggcaatcgca tctatctggt cgatcaaaat gaccgggtga
     9361 tggcgttgac cattgatggc ggcgttacgc tgtgggcaca aagcgatctg ctgcatcgcc
     9421 tgctgacttc tccggtgctg tataatggca acctggtggt cggtgacagt gaaggttatc
     9481 tgcactggat taacgtcgaa gatggtcgtt tcgttgccca gcaaaaagtt gatagttccg
     9541 gtttccagac tgaaccggtt gccgctgacg gcaaactgct gatccaggca aaagacggaa
     9601 ccgtgtactc tattacacgt taatcgtctc tgtcgttcac tttgaaaacg gctcctggac
     9661 aggggccgtt ttcctgtttt taacaacgac gcgaatatag cgtgcgttgt ctgatgattt
     9721 ataaaaatga ggctttaaac atggtacctg tggtcgcgct tgtcgggcgc cctaacgtag
     9781 gaaaatccac gttatttaac cgtctaactc gcacccgaga tgcgctggtt gcggatttcc
     9841 cgggtctgac tcgtgaccgt aagtacggtc gtgcggaaat tgaaggccgt gagtttatct
     9901 gtattgatac cggcgggatt gatggcacag aagacggtgt agaaacccgc atggcggaac
     9961 agtcgctgct ggcgattgaa gaagcggacg tcgtactgtt tatggtggat gcgcgcgcgg
    10021 gcctgatgcc ggcagatgaa gcgattgcca aacatctgcg ctcccgtgaa aaaccgacct
    10081 tcctggtggc aaacaaaact gacggtctgg atcccgatca ggcagtggtt gatttctact
    10141 cgcttggttt aggtgaaatc tacccgatcg ccgcgtctca cggtcgtggc gtattaagtc
    10201 tgctggagca tgtgctgctg ccgtggatgg aagatctcgc accgcaagag gaagtcgacg
    10261 aagacgctga atactgggcg caatttgaag cggaagagaa cggcgaagaa gaagaggaag
    10321 acgacttcga cccgcaaagt ctgccgatca aactggcgat tgtgggtcgt ccgaacgtag
    10381 gtaagtctac actcactaac cgtattcttg gagaagagcg cgttgttgtt tacgacatgc
    10441 ctggcacgac gcgtgacagc atctacatcc caatggaacg cgatggacgt gagtatgtgc
    10501 tcattgacac cgctggcgta cgtaaacgcg gcaaaatcac cgatgctgta gagaaattct
    10561 ccgtaatcaa aacgttgcag gccattgaag acgccaacgt ggtgatgtta gtgattgatg
    10621 cgcgcgaagg tatttccgat caggatctct cgctgctggg ctttattctc aatagtgggc
    10681 gctcacttgt cattgtggtg aataagtggg atggcctgag ccaggaagtg aaagagcagg
    10741 tgaaagaaac gctggacttc cgtctgggct ttatcgattt tgctcgtgtg cactttatct
    10801 ctgccttgca cggcagtggt gttggtaact tgtttgaatc agtacgtgaa gcgtatgaca
    10861 gctccacccg tcgtgtgggg acctctatgc tgacgcgcat catgacgatg gctgttgaag
    10921 atcaccaacc gccgctggta cgcggtcgtc gtgtgaagct gaaatatgcc cacgctggtg
    10981 gttataaccc gccgattgtg gtgattcacg gtaatcaggt gaaagacctg cctgattcct
    11041 acaagcgcta cttgatgaac tacttccgca aatcgctgga cgtaatggga tcgccgattc
    11101 gtattcagtt caaagaaggg gaaaacccgt atgcgaacaa gcgtaacacc ctgacgccaa
    11161 cccagatgcg taaacgtaag cgtctgatga agcacatcaa gaaaaataaa taattatccg
    11221 taagtatttt atctgccatt ccctctatga atgtagaggg aatggctacc ggaggaattc
    11281 atatggaact gcattgtcca aaatgtcagc atgtgcttga tcaggataac ggccatgcgc
    11341 gttgcccatc ctgcggggaa tttattgaaa tgaaagctct ttgtccagac tgccatcaac
    11401 cgttgcaggt gttaaaagcc tgcggtgcag tagattactt ctgtcagcac gggcatggct
    11461 tgatatcgaa aaaacgcgtt gaattcgtgc tggcttaatg aaccttttta cgcgattttt
    11521 ttaccggctg gatgtttttt acttcacttt ctatccagcc gtcttccaga cgtgtggtta
    11581 gcatttcacc cgctttaact tgcttcactt ttttcagtac attgccgtca gtagcagtag
    11641 taacgctgta tccacgcgcc agcgttgaca gtgggcttac ggcttcgagg tgcgttactg
    11701 cattaccgaa acgttcacgc gtggcgctaa gctgtgcgcg caggatttct gctaaacgat
    11761 attccagttg ctgaatgcgc gtttgcgtgc gatgaatctt cggctgtgga ttttgctgat
    11821 tcagccgctg tgttaatcgc tgctgctgtt gcccggcacg cttaagctga ttttccagcg
    11881 caaagctcat ccgtttttgc aggcgttcaa gcatggtttg ctggcgtgcc agccggagct
    11941 gcggatgctg ctgctgcaag cgatgatgaa tctgcgtaaa gcgacgcgta cggttggcga
    12001 gataataatc catcgccatc tccagccgtt gacgggccga ttgcacctgg cgcagtaact
    12061 cttgctgatt acggctcact acttcagcgg cggcagacgg cgttggcgca cgcagatcgg
    12121 caacaaaatc ggcaatggtc acatccgtct catgcccgac ggcgctgacg accggaatgc
    12181 ggctggcaaa aatcgcccgc gctacgcgtt cgtcgttaaa actccataaa tcttccagcg
    12241 aaccgccgcc gcgcccaacg atcaacacgt cgcactcatt gcgctgattc gccagttcaa
    12301 tggcgcgaac aatttgcccc ggcgcgtcat cgccctgaac ggaggtgggg tagatgatca
    12361 ccggtagaga aggatcgcga cgttttaaca catgcaaaat atcatgtagc gcagcaccgg
    12421 tttttgaggt gatcacacca acgcaatgcg caggggaggg aagtggtttt ttgtattgct
    12481 gatcgaacaa accttcagcc tgcaacttcg ctttgagctg ttcgtacttc tgttgcagca
    12541 gcccttcacc ggccggctgc atactctcaa cgattatctg gtagtcgccg cgcggctcgt
    12601 agagcgtaat attggcgcga actaaaactt gttgcccatg ctgcggacgg aaggtgaccc
    12661 ggcggttgct gttgcggaac atcgcgcagc gtacctgggc ggtgtcgtct ttgagtgtaa
    12721 agtaccagtg accggaagcc ggttgtgtga aattagagat ttcgccgctg atccaaacct
    12781 gtcccatctc atgctcaagc agcagacgaa ccgtttgatt caggcgacta acggtaaaaa
    12841 ttgcagggga ttgagaaggt aacatgtgag cgagatcaaa ttctaaatca gcaggttatt
    12901 cagtcgatag taacccgccc ttcggggata gcaagcattt tttgcaaaaa ggggtagatg
    12961 caatcggtta cgctctgtat aatgccgcgg caatatttat taaccactct ggtcgagata
    13021 ttgcccatgc tacgtatcgc taaagaagct ctgacgtttg acgacgttct cctcgttcct
    13081 gctcattcta ccgttctgcc gaatactgct gacctcagca cccagctgac gaaaactatt
    13141 cgtctgaata tccctatgct ttccgcagca atggataccg taacggaagc gcgcctggct
    13201 attgctctgg ctcaggaagg cggtatcggc tttatccaca aaaacatgtc cattgaacgc
    13261 caggcagaag aagttcgccg tgtgaaaaaa cacgaatctg gtgtggtgac tgatccgcag
    13321 actgttctgc caaccacgac gctgcgcgaa gtgaaagaac tgaccgagcg taacggtttt
    13381 gcgggctatc cggtcgttac cgaagaaaac gaactggtgg gtattatcac cggtcgtgac
    13441 gtgcgttttg ttaccgacct gaaccagccg gttagcgttt acatgacgcc gaaagagcgt
    13501 ctggtcaccg tgcgtgaagg tgaagcccgt gaagtggtgc tggcaaaaat gcacgaaaaa
    13561 cgcgttgaaa aagcgctggt ggttgatgac gaattccacc tgatcggcat gatcaccgtg
    13621 aaagacttcc agaaagcgga acgtaaaccg aacgcctgta aagacgagca aggccgtctg
    13681 cgtgttggtg ctgcagttgg cgcaggtgcg ggtaacgaag agcgtgttga cgcgctggtt
    13741 gccgctggcg ttgacgttct gctgatcgac tcctcccacg gtcactcaga aggcgttttg
    13801 cagcgtatcc gtgaaacccg tgctaaatat ccggatctgc aaatcatcgg cggcaacgtg
    13861 gcaacagctg caggtgcacg cgctttggca gaagctggtt gcagcgcggt taaagtcggt
    13921 atcggccctg gttctatctg tacgactcgt atcgttactg gcgtaggtgt tccgcagatc
    13981 actgccgttg ctgacgcagt tgaagccctg gaaggcaccg gtattccggt tatcgcagat
    14041 ggtggtattc gcttctccgg cgacatcgcc aaagctatcg ccgctggcgc aagcgcggtg
    14101 atggtaggtt ccatgctggc gggtactgaa gaatctccgg gtgaaatcga actctaccag
    14161 ggccgttctt ataaatctta ccgtggtatg ggttccctgg gcgcgatgtc caaaggttcc
    14221 tctgaccgtt atttccagag cgataacgct gccgacaaac tggtgccgga aggtatcgaa
    14281 ggtcgcgtag cttataaagg tcgcctgaaa gagatcattc accagcagat gggcggcctg
    14341 cgctcctgta tgggtctgac cggctgtggt actatcgacg aactgcgtac taaagcggag
    14401 tttgtacgta tcagcggtgc gggcattcag gaaagccacg ttcacgacgt gaccattact
    14461 aaagagtccc cgaactaccg tctgggctcc tgattctctt cgcccgactt catgtcgggc
    14521 gatttatatt atctgtttca cttgcctcgg aataagcgtc aatgacggaa aacattcata
    14581 agcatcgcat cctcattctg gacttcggtt ctcagtacac tcaactggtt gcgcgccgcg
    14641 tgcgtgagct gggtgtttac tgcgaactgt gggcgtggga tgtgacagaa gcacaaattc
    14701 gtgacttcaa tccaagcggc attattcttt ccggcggccc ggaaagtact actgaagaaa
    14761 acagtccgcg tgcgccgcag tatgtctttg aagcaggcgt accgatattc ggcgtttgct
    14821 atggcatgca gaccatggca atgcagttgg gcggtcacgt tgaagcctct aacgaacgtg
    14881 aatttggcta cgcgcaggta gaagtcgtaa acgacagcgc actggttcgc ggtatcgaag
    14941 atgcgctgac cgcagacggt aaaccgctgc tcgatgtctg gatgagccac ggcgataaag
    15001 ttaccgctat cccgtccgac ttcgtcaccg tagccagcac cgaaagctgc ccgtttgcca
    15061 ttatggctaa cgaagaaaaa cgcttctatg gcgtacagtt ccacccggaa gtgactcaca
    15121 cccgccaggg tatgcgcatg ctggagcgtt ttgtgcgtga tatctgccag tgtgaagccc
    15181 tgtggacgcc agcgaaaatt atcgacgatg ctgtagctcg catccgcgag caggtaggcg
    15241 acgataaagt catcctcggc ctctctggtg gtgtggattc ctccgtaacc gcaatgctgc
    15301 tgcaccgtgc tatcggtaaa aacctgactt gcgtattcgt cgacaacggc ctgctgcgtc
    15361 tcaacgaagc agagcaggtt ctggatatgt ttggcgatca ctttggtctt aacattgttc
    15421 acgtaccggc agaagatcgc ttcctgtcag cgctggctgg cgaaaacgat ccggaagcaa
    15481 aacgtaaaat catcgggcgc gttttcgttg aagtattcga tgaagaagcg cagaaactgg
    15541 aagacgtgaa gtggctggcg cagggcacca tctaccctga cgttatcgaa tctgcggctt
    15601 ctgcaaccgg taaagcacac gtcatcaaat ctcaccacaa cgtgggcggc ctgccgaaag
    15661 agatgaagat gggcctggtt gaaccgctga aagagctgtt caaagacgaa gtgcgtaaga
    15721 ttggtctgga gctgggcctg ccgtacgaca tgctgtaccg tcatccgttc ccgggaccag
    15781 gccttggcgt tcgtgttctg ggtgaagtga agaaagagta ctgtgacctg ctgcgccgtg
    15841 ctgacgccat cttcattgaa gaactgcgta aaacggacct gtacgacaaa gtcagccagg
    15901 cgttcaccgt cttcctgcca gtacgttccg ttggcgtaat gggcgatggt cgtaagtatg
    15961 actgggttgt ctctctgcgt gctgtcgaaa ccatcgactt tatgaccgca cactgggcgc
    16021 atctgccgta cgatttcctc ggtcgcgttt ccaaccgcat tatcaatgaa gtgaacggta
    16081 tttcccgcgt ggtgtatgac atcagcggca agccgccagc taccattgag tgggaatgat
    16141 tttgatccct gcactatgaa tgaacaaaac cctccgttac tacagagggt tttttatctt
    16201 caagaatcat aggcttgaag ttactaacat cgattaatta aaccagctgt ccgatttgtt
    16261 ctcttctgct ttgcccacgc ttttcatcag atcgcgaccg ccttcagtca tatttctgtt
    16321 tgcgtcagct tcagattgca ccacatcggt ttgcgcagct ttgtgcttca gttcctgatc
    16381 gataaattcg ttttcgcgct taacgcgggc ttcttctttc gccagcgcca gtttttgttt
    16441 ctgaatctct aagctgcgta gctcatcttc ataactttga tcgcgttttt tgtccgcagt
    16501 ggcttcggcg tccagtttat cctgacgagc tttcttattt gccgctgccg ttgccgctct
    16561 tttgttcgcg gcggcctggg catttgcgcg acgttgcttc tcttgctgga tttccctgtt
    16621 gcgctccgcg acccattcgt catgctgcct ttgctcttca tttttacctt gctgttccgc
    16681 ttctgcgaca gcagagagtt gatcctgcaa tgatgaggct atagccggat agcttaagga
    16741 ggctaagatg gcgcaaagaa aaactttctt catgactcct cctgattatt agctcttttc
    16801 aggacatttg gtatttggct gaatacgcgt ttcgttatac gttgtggtaa taacaacggc
    16861 taaacctgtc gtaaactggc actctttacc ctcctgggtg gaggtataca ctttggtgcc
    16921 ttccttatat gttaaagaaa caccttccac taaggtttta tcattcacca tagaacccgc
    16981 tgccgcgcct acagctccgc cgccaactgc ccctgccgtc gttccggaat tgctgccaga
    17041 accgacgttg tggccgataa caccgccagc gactgcgcca ataagcgcgc cgaaggcttg
    17101 tgcgttccgt ttattttggg agttgtctac ggcaactttt gcgggaagaa tggaaataat
    17161 attaacggtt ttagtttctt gtttggtatt cagttgatcg gtttgataaa catcggcggc
    17221 atgatcgtca gcatttgact ggcatcctgc cagagtgaat gacgctaaca ttgccacagg
    17281 cagaagacat tttttaaatt tcatcactat tccttgttat acacattacg aaaaataaga
    17341 catttaagat catcaaaaga ggaaagtaaa tgtattgcgt ataaagggat agattaaatt
    17401 tattaaaatt cacgcaggga ataatttata aaaatatatc attacaacat atatattaat
    17461 tttatattta tttcgcagcc aaacaggata aacatcagtc aggacaaaga cgtaaaattt
    17521 aagatgaaag aatatctgcc gctttgataa tcccaaagag caaaagcatc cgacagcgaa
    17581 gtgccgttat gttctgtgaa agtggagaag gtaaccgcta acgttgtacc ggtgattcga
    17641 cggagagtgt ggattgctga gcttcttttt tgctctgatg gtgcagccag gcaccgacgg
    17701 acgagtagat aaaacggcca aagaagaaga taaagctgat gagcagtacg atacgggtca
    17761 tgcgactgtt aaatcggtgt cgttttcgca tactagttgc ctgactcaca aaaggttcct
    17821 tgaagtatgt cccacgctgt ggacggtact tacattaagg cacaacagga caaaatggtc
    17881 aattcttcgt tatgtaaaaa agcgtcagtg gatacatatt ttaatgttat ggaagttaat
    17941 ttaattattt acaatgatga tgtaataatg atgaattaac atgataaacg taataattca
    18001 ttaatcaggg ttattttgtt tgatatatat caattggaac tttcatatga cacttagaat
    18061 caatgctctc tatagtgatg aataatcatc attcgaagtc aggtgggatg cctgtctgaa
    18121 tacacctcct tcaggatgtg ggggattctg ctgagcatct atgaaactga atg
//
LOCUS       DAETPZ010000076        17461 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.076, whole genome shotgun sequence.
ACCESSION   DAETPZ010000076 DAETPZ010000000
VERSION     DAETPZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.076"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            564..1334
                     /locus_tag="K0W59_002955"
     CDS             564..1334
                     /locus_tag="K0W59_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HBI3023907.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="K0W59_002956"
     CDS             1325..1798
                     /locus_tag="K0W59_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HBI3023908.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGTLSIGLSIPGLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="K0W59_002957"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="K0W59_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HBI3023909.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="K0W59_002958"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="K0W59_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HBI3023910.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGVIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="K0W59_002959"
     CDS             3233..3727
                     /locus_tag="K0W59_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HBI3023911.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="K0W59_002960"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="K0W59_002960"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HBI3023912.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="K0W59_002961"
     CDS             complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="K0W59_002961"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HBI3023913.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7571..8484
                     /locus_tag="K0W59_002962"
                     /pseudo
     CDS             7571..8484
                     /locus_tag="K0W59_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            8699..10060
                     /locus_tag="K0W59_002963"
     CDS             8699..10060
                     /locus_tag="K0W59_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3023914.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="K0W59_002964"
     CDS             complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="K0W59_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HBI3023915.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="K0W59_002965"
     CDS             complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="K0W59_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HBI3023916.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12610..13899
                     /locus_tag="K0W59_002966"
     CDS             12610..13899
                     /locus_tag="K0W59_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HBI3023917.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13896..15362
                     /gene="hpaE"
                     /locus_tag="K0W59_002967"
     CDS             13896..15362
                     /gene="hpaE"
                     /locus_tag="K0W59_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HBI3023918.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTANRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATERNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKLSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15364..16215
                     /gene="hpaD"
                     /locus_tag="K0W59_002968"
     CDS             15364..16215
                     /gene="hpaD"
                     /locus_tag="K0W59_002968"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HBI3023919.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16225..16605
                     /locus_tag="K0W59_002969"
     CDS             16225..16605
                     /locus_tag="K0W59_002969"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HBI3023920.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16673..>17461
                     /gene="hpaH"
                     /locus_tag="K0W59_002970"
     CDS             16673..>17461
                     /gene="hpaH"
                     /locus_tag="K0W59_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HBI3023921.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSIS"
BASE COUNT         4294 a         4627 c         4378 g         4162 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgttt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tggggtctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtacgtt
     1741 atccatcggt ctttccattc ctggattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgatcaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggtg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaggcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact aaatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgcccg ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc tgacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cccaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tctccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcgt
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacgattt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg cttcagctgc cgacaatatc ttttttgatc aatcgcttga gatcatctca
     8281 gtcatctttc attttcacta tcaatgggac gaaagcgatc ttaaacagcg caatatcatt
     8341 gccgtcgatg aacatatgac cattctcagc gctctgattt gccgcagtga ccttgatgcc
     8401 acccttgcgc tccgtaacca tttgaattca gctaaacaat caatgattcg atccattaat
     8461 gagaacacgc gttatgctca ttaagtacgg attaaaaaca gcaaactatt agcgggtgca
     8521 cggttttatc aaaagataaa taccgggaaa ttcccgctta cctatgctca caatcagtcg
     8581 actaaaaatg tttttgtgag cggtaataaa gtcctaaaac tttaacctgc cgttaatgtc
     8641 aaccttgcgt aatgcacaat tacgtaaaca attaaaatta taaatacagg agtccggcgt
     8701 ggaaaaagaa aatatcaccc tcgatccgcg ttcttcattt actccatctt cgtcggcaga
     8761 tattcccgtg ccaccagatg gattagttca acgcagtacc cgaattaaac gcattcaaac
     8821 caccgccatg ttgttattat tttttgcggc ggtaatcaat tatctcgacc gcagttcgct
     8881 gtcggtagca aatttaacga ttcgtgaaga attgggatta agtgccaccg aaatcggcgc
     8941 tttgctctcc gtgttttcac tcgcttacgg cattgcgcaa cttccttgcg gcccactatt
     9001 ggatcgtaaa ggcccacgcc tgatgctggg actggggatg ttcttctggt cactgttcca
     9061 ggccatgtct ggcatggtgc acagctttac gcagttcgtg ttggtgcgta tcggtatggg
     9121 gattggtgaa gcgccgatga acccatgcgg tgtaaaagtc attaacgact ggttcaacat
     9181 caaagagcgc ggacgcccga tgggcttctt caacgcagct tctaccattg gcgttgcggt
     9241 aagcccaccg attctggcgg caatgatgct ggtgatgggc tggcgcggga tgtttattac
     9301 cattggtgta ctggggattt ttctcgccat cggctggtat atgctctatc gcaaccgcga
     9361 gcacgtagaa ctgactgccg ttgaacaagc ttatctcaat gcaggtagcg tcaatgcccg
     9421 ccgagatccg ctcaattttg ccgaatggcg cagcctgttc cgtaaccgca caatgtgggg
     9481 aatgatgctc ggattcagtg gcatcaacta cactgcgtgg ctgtatctgg cctggcttcc
     9541 tggttacctg caaacagcct ataacctgga tttaaaaagc acagggttga tggcggctat
     9601 ccctttcctg tttggggctg ccgggatgct ggtcaacggt tacgttactg actggctggt
     9661 caaaggggga atggctccga ttaaaagccg taagatctgc attattaccg ggatgttctg
     9721 ttctgccgcc tttacgctga tagtaccaca agcgacaaca tccatgatgg cggttctgct
     9781 gattggcatg gcactgttct gtattcactt tgccggaaca tcctgctggg gcttgatcca
     9841 cgtcgcagtt gcttctcgca tgactgcgtc ggtgggcagt atccagaact ttgccagctt
     9901 catctgcgcc tctttcgcgc cgatcattac tggttttatt gttgatacaa cccactcatt
     9961 ccgtctggca ctaatcattt gcggttgcgt caccgcagcg ggagcactgg cgtacatctt
    10021 cctggttcgc cagccgatca acgacccgcg taaagattaa actccctatg cccgataaca
    10081 ttttgcttat cgggcatttt tatggcgatt aaaacgtttc ccagttctcc ccgctatctg
    10141 ccactgccat tttacgcggc gtagctggcg tcacggtttt taccacagcc gatgtttcac
    10201 gctgctgttg ctgctgttgc tgaatccgga acactgccac agcttcggtc aggcgactgg
    10261 cctgctcttc cagcgcggcg gcggcagcgg cagattcttc caccagcgcg gcgttctgtt
    10321 gagttacccg gtccatctca gcaaccgcta agccaacctg atcgatacca cggctctgct
    10381 catcagaagc agaagctatt tcgcccataa tgtccgtcac gcgggtcaca gcactgacaa
    10441 tctccgccat tgtttccccg gcgctttcga ccagcgtaga gccaacatcc actttcccca
    10501 ccgagtcttc aatcaggctt ttaatttcac gagccgcctg ggcgctacgc tgggccagat
    10561 tacgcacttc tcccgcaacc accgcaaaac cacgtccttg ctcacccgca cgcgctgctt
    10621 caaccgccgc gttcaaagcc agaatattgg tctggaaggt aatgccgtca attacgctga
    10681 taatatcggc gattttctgc gaactggtgg agatatcgcg catagtctgc accacgttat
    10741 ctaccacttt gccgccgcgt tgcgccgttt cagaagcact taatgccaga tggctggcct
    10801 ggcgcgcatt ctcggcgttc tgtttcaccg ttgcggtcag ttgctccatg ctggctgccg
    10861 tctcttccag cgaagcggcc tgttgctcgg tgcgcgaaga gagatcatta ttgccggtag
    10921 cgatttcgct ggcaccgcta tagatggcat tggccccgtt gcgcacatca ccgacggtac
    10981 gcatcagctc tccctgcata tggcgcaaac tctctgccag ttgccccatc tcattagagc
    11041 catccacctc aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca
    11101 ggcgattcat tggcgctacc agcgaggctt taataccgaa ccagacggcg aagatgaccg
    11161 ccagtacgac gatcatcacg cccacaagaa tccacatcgc ctggctgtag gaggcattgt
    11221 tatcgctgac ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct
    11281 tctcgaaacc gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg
    11341 cacctaacag ttggatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga
    11401 tctctgccgc tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc
    11461 agtttttttc cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg
    11521 aaccgctacc aatattattc tgatccatca tgtagcggat acccgcgcgg ttgagggtgt
    11581 tacgcgtctg caacaacgcg acccagctgc cattcagcgt ggattgctgc tggcgaatgg
    11641 tttgtaaaac agtgaaattt tctttgtcat tctttaaggc attaaaaaac agaccgcctg
    11701 atgtcagttg taaaaggcca aaaacggcca aaaccagcag taagctggtc acgatcttga
    11761 tacgttttaa catggtttct ctttcctgtg gaccgatgca agattttcgg cctggaaagc
    11821 aaaaacttta tgaaaaggtg taacccgcca tccatggaaa aaccaggcgc tggttatcag
    11881 atactaaaaa gttattcatt atcgcccggg atgtcttctt gtcgggaatt gcccagagca
    11941 ataaattcct ctaacagatg ggttaactgt tgcattttct cggcagtaaa ctgagcttca
    12001 atctgccgat aggcttcttc aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct
    12061 ttggtcagcg agatatagag cttccgctga tcattgatcg gctttaatcg caacactaaa
    12121 ccgtcgcgtt ccatccgcgt gaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga
    12181 tacgccagat cgtgaaaatc cattgatggg ctttccgcca gaatacgcac gatgcgccac
    12241 tgttgctcag tcaaattatg ccgtttaacg attgggcgaa aataactcat cgccgcttct
    12301 cgcgcctgga gcaacgcaat ggttagtgag tcgtgcatta tctttcccct gaaaaattca
    12361 aaatcattaa tatagaaaca attaatcatc acgttaccgg gactgagctg cccccgcaat
    12421 tcttcatttt atcaaaattg atgatatttt attaacttac tgatatttaa tattttatta
    12481 tctttaaatg taaatagttt gttaattaga tcacatttat atcacttact tttgccaaac
    12541 tacgcaatcg ggaataaaag taatcattaa catattaatg attaagatga tcgaatccga
    12601 ggagtggtaa tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat
    12661 gcctggcagg aagcgttcca gcaatctccc tacaaagccc cgcctaaaac tgcggtctgg
    12721 tttattaaac cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt
    12781 gaaaaagtac tgagcggtgc gactgttgcg ctgattgtgg ggaaaacggc gacgaaagta
    12841 cgtgaagaag atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg
    12901 ccggaagaga gcttttaccg tccggcaatc aaagcaaaat gtcgtgatgg attctgcccc
    12961 attggcgaaa ccgtggctct cagcaatgtc gataatctga ctatctatac cgagatcaac
    13021 gggcgtcctg ccgatcactg gaataccgcc gatttacaac gtaacgccgc acagctgctg
    13081 agcgccctga gcgaatttgc cacgctgaat ccaggcgatg ctattctgct cggcacgcca
    13141 caggcgcgcg tggaaataca gccaagcgat cgcgtgcgtg ttctcgcaga aggtttcccg
    13201 ccgctggaaa atccggtagt ggacgaacgt gaagtgacca cgcgcaagag cttcccaacg
    13261 ctgccacacc cgcacggtac gctgtttgcc ctcggcctga actacgccga ccacgccagc
    13321 gaactggaat ttaagccacc ggaagagccg ctggtgttcc tgaaagcgcc gaataccctc
    13381 actggcgaca accagacctc cgtgcgcccg aacaatattg aatacatgca ctacgaagcg
    13441 gagctggtgg tagttattgg caagcaggcg cgtaacgtca gcgaagccga tgccatggat
    13501 tatgtcgcgg gctacaccgt gtgtaacgac tacgccattc gcgactatct ggaaaactac
    13561 taccgcccta acctgcgggt caaaagccgc gacggactga cgccgatgct ttcaaccatc
    13621 gtgccgaaag aggcgatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc
    13681 gagttacgcc agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc
    13741 tacttaagcg aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacaccaaaa
    13801 ggcttatctg acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc
    13861 ctggtgaacc gaattgtgag tgaggaaaca gcgaaatgaa aaaagtaaat cattggatca
    13921 acggcaaaaa tgttgcaggt aacgactact tccagactac caatccggca acgggtgaag
    13981 tgctggcgga tgtggcctct ggcggtgaag cggagatcaa tcaggcggta gcggcagcga
    14041 aagaggcgtt cccgaaatgg gctgatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc
    14101 gtctgggcga tctgatcgac cagaacgtgc ctgagatcgc cgcgatggaa accgcggaca
    14161 cgggcctgcc gatccatcag accaaaaatg tgttgatccc acgcgcttct cacaactttg
    14221 aatttttcgc ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga
    14281 tgctcaacta cacgctggtg caaccggtag gcgtttgtgc gctggtgtcg ccgtggaacg
    14341 tgccgtttat gaccgccacc tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg
    14401 tgctgaagat gtcggaactc tccccgctga ccgccaaccg cttgggtgag ctggcgctgg
    14461 aagctggtat tccggcgggc gtgctaaacg tggtacaggg ctacggcgca accgcaggcg
    14521 atgcgctggt ccgtcatcat gacgtgcgtg ccgtgtcgtt caccggcggt accgccaccg
    14581 agcgcaacat catgaaaaac gccgggctga aaaaatactc catggagctg ggcggtaaat
    14641 caccggtgct gatttttgaa gatgccgata ttgagcgtgc gctggacgcc gccctgttca
    14701 ccatcttctc aatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcaacaga
    14761 gcatctatcc agaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtgggcg
    14821 atccgaacga tccgaatacc caggttaggg cgcttatcag tcagcaacac tgggaaaaag
    14881 tctccggcta tatccgtctc ggcattgaag aaggcgcaac cctgttggcg ggcggcccgg
    14941 ataaactgtc cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaacggtgc
    15001 tggcggacgt agataaccgt atgcgcgttg cccaggaaga gattttcggg ccggtcgcct
    15061 gcctgctgcc gtttaaagac gaagccgaag gcttacgcct ggcaaacgac gtggagtacg
    15121 gcctcgcgtc gtacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca
    15181 ttgaagcagg catggtgttc gtcaacaccc agaacgtgcg cgacctgcgc cagccatttg
    15241 gcggcgtaaa agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg
    15301 cggaaatgaa gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct
    15361 gatatgggta agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatctctct
    15421 gaactaccag gaaaaaacca cggttgtcgc cagggtgcga tcgacgggca taaagagatc
    15481 agcaagcgtt gccgggaaat gggcgtcgat accattatcg ttttcgatac tcactggctg
    15541 gtcaacagtg cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac
    15601 gagctgccgc attttattcg cgacatgacc tacaactacg agggcaaccc ggagttgggg
    15661 cagctaattg ccgaggaagc cttaaagctc ggcgtgcggg caaaagcgca caatattccc
    15721 agcctgaaac tggaatacgg cacgctggta ccgatgcgct acatgaatga agacaagcac
    15781 ttcaaagtgg tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag
    15841 ctgggcgaag cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc
    15901 agcggttcgt tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc
    15961 tacacccgcg agttcgaccg ccagatggac gagcgtgtgg tgaagctgtg gcgcgaaggc
    16021 cagttcaaag agttttgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc
    16081 aatatgcacg acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag
    16141 gtggagttta ttaccgaact gttcccaagc tctggcaccg gtcaggttaa cgctgttttc
    16201 ccgctgcccg cgtaaggacg ttttatgccg cactttatcg tggaatgtac tgaaaatatt
    16261 cgtgaagaag cccgcttacc tgagctgttt gccagtgtta acactgcact ggcggcaacg
    16321 gggatttttc cgcttggggg aatacgtagc cgggcgcact ggatcgatac ctggcaaatg
    16381 gctgatggca agcacgatta cgcctttgtg catatgacgc taaaaatcgg cagcggacgc
    16441 agtctggaaa gccgtcagga agtgggcgaa atgctgtttg atctgattaa aacgcacttc
    16501 gcctcactga tggagagccg ttacctagcg ctttcatttg agattgcaga gctgcacccg
    16561 acgttgaatt tcaaacagaa caacgtacac gcgttgttta aataaatttg caccagcttc
    16621 caggccggat gataaggcgc tgccgcatcc ggcaatgcca caggatatcg ctatgttcga
    16681 taaacacacc cacaccctga tcgcccagcg tctggatcag gcagaaaaac agcgcgaaca
    16741 gatccgcgcg atctcgctgg attacccgga gatcaccatc gaagacgcct acgccgtgca
    16801 gcgcgaatgg gttcgcctga aaatcgccga aggtcgcacg ctgaaaggcc acaaaatcgg
    16861 tctgacctcg aaagcgatgc aggcaagctc gcagatcagc gaaccggatt acggtgcact
    16921 gctggacgac atgttcttcc acgatggcag cgatatcccg accgatcgct ttatcgtgcc
    16981 gcgcattgaa gtggagctgg cttttgtgct ggcaaaaccg ctgcgtgggc caaactgcac
    17041 gctgttcgac gtttacaacg ccacggacta tgtgatcccg gcgctggagc tgatcgacgc
    17101 tcgctgccac aacatcgatc cggaaaccca gcgcccgcgc aaagtattcg acaccatttc
    17161 cgataacgcc gccaacgccg gagtgatcct cggaggtcgt ccgatcaaac cagacgaact
    17221 ggatctgcgt tggatctccg ccctgatgta tcgcaatggc gtgattgaag aaaccggcgt
    17281 cgccgctggt gtgctgaatc atccggcaaa cggcgtggcc tggctggcga acaaactcgc
    17341 accgtatgac gtacaactgg aagccgggca aatcattctc ggtggttcgt tcacccgccc
    17401 ggttccggcg cgtaagggcg acaccttcca cgtcgattac ggcaacatgg gctccattag
    17461 c
//
LOCUS       DAETPZ010000077        17379 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.077, whole genome shotgun sequence.
ACCESSION   DAETPZ010000077 DAETPZ010000000
VERSION     DAETPZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.077"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(329..673)
                     /locus_tag="K0W59_002971"
     CDS             complement(329..673)
                     /locus_tag="K0W59_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBI3023922.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            805..2715
                     /locus_tag="K0W59_002972"
     CDS             805..2715
                     /locus_tag="K0W59_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBI3023923.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSLDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2773..3468
                     /gene="tsaB"
                     /locus_tag="K0W59_002973"
     CDS             2773..3468
                     /gene="tsaB"
                     /locus_tag="K0W59_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBI3023924.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3508..4089
                     /gene="yeaY"
                     /locus_tag="K0W59_002974"
     CDS             3508..4089
                     /gene="yeaY"
                     /locus_tag="K0W59_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HBI3023925.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4294..5979
                     /gene="fadD"
                     /locus_tag="K0W59_002975"
     CDS             4294..5979
                     /gene="fadD"
                     /locus_tag="K0W59_002975"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HBI3023926.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            6061..7176
                     /gene="rnd"
                     /locus_tag="K0W59_002976"
     CDS             6061..7176
                     /gene="rnd"
                     /locus_tag="K0W59_002976"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HBI3023927.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRADWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMFNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHRKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="K0W59_002977"
     CDS             complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="K0W59_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HBI3023928.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIETVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8251..8868)
                     /gene="yeaW"
                     /locus_tag="K0W59_002978"
                     /pseudo
     CDS             complement(8251..8868)
                     /gene="yeaW"
                     /locus_tag="K0W59_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(9210..9761)
                     /locus_tag="K0W59_002979"
     CDS             complement(9210..9761)
                     /locus_tag="K0W59_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3023929.1"
                     /translation="MRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTYPSLDR
                     LAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELLEDKEL
                     HSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRKKILSK
                     YGIYEQEGLVAPSQEPQDVKKAV"
     gene            9987..10214
                     /locus_tag="K0W59_002980"
     CDS             9987..10214
                     /locus_tag="K0W59_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3023930.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSREAGLKPDTMRNVFRC
                     HIPRYEAIIARYLDMEPAQIWPSRYMEKTSC"
     gene            10208..10639
                     /locus_tag="K0W59_002981"
     CDS             10208..10639
                     /locus_tag="K0W59_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023931.1"
                     /translation="MLTHWVTAQECVGMSCFPKHEVSIRRNLEKLAANHPELRRKRTG
                     SKVLEFHISVLPAAARVELLLKSEQIETSQGYFEIARPTLEAHDYDREALWSKWDKAS
                     DSQRRLAEKWLPAVQTADEMLQPLLVRLSCYLSTGRLKVNA"
     gene            complement(10691..10765)
                     /gene="yoaJ"
                     /locus_tag="K0W59_002982"
     CDS             complement(10691..10765)
                     /gene="yoaJ"
                     /locus_tag="K0W59_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HBI3023932.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(10768..10866)
                     /locus_tag="K0W59_002983"
     CDS             complement(10768..10866)
                     /locus_tag="K0W59_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HBI3023933.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            complement(10899..11924)
                     /gene="dgcP"
                     /locus_tag="K0W59_002984"
     CDS             complement(10899..11924)
                     /gene="dgcP"
                     /locus_tag="K0W59_002984"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HBI3023934.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            12107..12361
                     /gene="yoaF"
                     /locus_tag="K0W59_002985"
     CDS             12107..12361
                     /gene="yoaF"
                     /locus_tag="K0W59_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HBI3023935.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            complement(12383..12730)
                     /locus_tag="K0W59_002986"
     CDS             complement(12383..12730)
                     /locus_tag="K0W59_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBI3023936.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(12785..13966)
                     /gene="nimT"
                     /locus_tag="K0W59_002987"
                     /pseudo
     CDS             complement(12785..13966)
                     /gene="nimT"
                     /locus_tag="K0W59_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            14063..14884
                     /gene="nimR"
                     /locus_tag="K0W59_002988"
     CDS             14063..14884
                     /gene="nimR"
                     /locus_tag="K0W59_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HBI3023937.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            complement(14841..15287)
                     /locus_tag="K0W59_002989"
     CDS             complement(14841..15287)
                     /locus_tag="K0W59_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBI3023938.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            15457..15561
                     /locus_tag="K0W59_002990"
     CDS             15457..15561
                     /locus_tag="K0W59_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023939.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            complement(15562..16065)
                     /gene="yeaK"
                     /locus_tag="K0W59_002991"
     CDS             complement(15562..16065)
                     /gene="yeaK"
                     /locus_tag="K0W59_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HBI3023940.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNTGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(16108..17211)
                     /locus_tag="K0W59_002992"
     CDS             complement(16108..17211)
                     /locus_tag="K0W59_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBI3023941.1"
                     /translation="MSVKLRLPHAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHKY
                     PGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGIAEEKFHYYLDLN
                     DRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQHV
                     MSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIIN
                     QSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFR
                     LYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDNL
                     GHQEGDLAITLHDAYKASDERLYVNKQNKNSRS"
     gene            17250..>17379
                     /locus_tag="K0W59_002993"
     CDS             17250..>17379
                     /locus_tag="K0W59_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3023942.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQ"
BASE COUNT         4469 a         4217 c         4401 g         4292 t
ORIGIN      
        1 agttaacctg ttgacattca ggtgttttaa aggtaacagc tcatggtgtg ttatccagta
       61 agatggagaa aattaacggt cagcccgcac gctataacca gattgttcag ctaagaaaga
      121 aaaccagagt tgctaatcaa agggcgaaca caacggcatg gttatgttta tttatatagt
      181 tagttttatg gataaaattg aaattataaa tataaataat gaattagcag aagcatcaac
      241 agtgatatct tattctctgg tcgatttatg taaaaccgac cagtattatc gataaattat
      301 cgtctgactt attttgcttc gataaagctt acaccgcagc cacaatctta atttcaactt
      361 tatacttcgg gttcatcaaa cccgcttgta ccgtgcagcg caccggcgca tgacccgcga
      421 caacccaggc atcccacgct ttattcatcg ccgcgaagtc gtttttatcg gccaggaaaa
      481 tggtggcatc cagaatgctc gatttattgc tgccctgttt ttccagcacg gcgtcaatct
      541 gtgccagcgt gttggcggtt tgctcaaagg catcggcgtc gaggttttcc ggtacaccag
      601 tgtagtagag cgtgttgttg tggattacta catcagacca gcgggcttca gcatcgatac
      661 gaacgatagt catcattttg tcctcattat acttccattt tcgtgctgca aagactgcca
      721 caatgtgagg ttgccgtcac cacttcaact ggcgaaagcg ccctcatcct gacataatcc
      781 ctgttcaaat caacaggggg tagtgtgacg gacgattttg caccagacgg tcagctggcg
      841 aaagcgatac caggctttaa gccgcgagaa ccacagcgac agatggcggt agccgtcacc
      901 caggcgatag aaaaaggcca gccgctggtg gtggaagcag gaaccggtac gggcaaaacc
      961 tacgcttacc tggctcctgc gctgcgggcg aaaaagaaag tcattatctc gaccggctca
     1021 aaagcgttgc aggatcagct ctacagccgc gatttgccaa cagtctcaaa ggcattgaaa
     1081 tatacgggca acgtggcgct gctgaaaggg cgctcaaact acctctgcct cgaacgtctc
     1141 gaacagcagg cgctggcggg gggcgatctg ccggtacaaa tcttaagcga tgtgatcctg
     1201 ctgcgctcct ggtctaatca aacagtcgat ggtgatatca gcacctgcgt cagcgtggcg
     1261 gaagattcac aggcgtggcc gctggtcacc agcaccaacg acaactgtct tggcagcgac
     1321 tgcccgatgt ataaagattg ctttgtggtc aaagcacgta aaaaagcgat ggacgccgat
     1381 gtggtggtgg taaaccatca tctctttctg gcggatatgg tggttaaaga gagtggattt
     1441 ggcgagttga tccctgaagc ggacgtcatg atcttcgacg aagcccacca gctaccggac
     1501 attgccagcc agtattttgg tcagtcactc tccagtcgac aactgctcga cctggcaaaa
     1561 gacatcacca tcgcctaccg caccgaatta aaagacaccc agcagttaca aaagtgcgct
     1621 gatcgtcttg cccagagtgc gcaggatttt cgtctgcaac tcggtgagcc aggttatcgc
     1681 ggtaacctgc gtgagctgtt agctaatccg caaattcagc gggcattttt actgctcgat
     1741 gacaccttgg aactttgtta tgacgtggcg aaactgtcac tggggcgttc cgccttgctg
     1801 gatgcggcat ttgagcgcgc cacgttgtat cgcacacggc tgaagcggct aaaagagatc
     1861 aatcagccgg gctacagcta ctggtacgaa tgcacttcgc gccattttac tctggctctc
     1921 acgccgctca gcgtggcgga taaattcaaa gagttaatgg cgcaaaagcc cggtagctgg
     1981 atcttcacct cagcaacgct gtcggtgaac gacgatctgc atcatttcac ctcgcggctt
     2041 gggatagaac aggcggagtc gttgctattg ccaagcccgt ttgattacag ccgccaggcg
     2101 ttactctgtg tgccgcgcaa tctgccgcaa accaaccagc caggttctgc tcgccagtta
     2161 gcggcaatgc tgcgaccgat catcgaagct aacaacggtc gttgttttat gctttgtacc
     2221 tcgcacgcca tgatgcgcga tctggccgag cagttccgcg ctaccatgac gcttcccgtt
     2281 ttgttgcagg gggaaaccag taaaggccaa ctgttgcagc agtttgtcag tgccggtaat
     2341 gcgcttcttg tggcaaccag tagtttctgg gaaggggtgg acgtgcgtgg cgatacattg
     2401 tcattggtaa ttatcgacaa attgccgttt acctcgctgg atgatccact gttaaaagcg
     2461 cgcatggaag attgtcgttt gcgcggtggc gacccgttcg atgaagtgca actaccagat
     2521 gccgtcatta ctctcaaaca gggggtaggt cgactgattc gcgacgccga cgatcgtggc
     2581 gtgctggtga tttgtgacta tcggctggtg atgcgtcctt acggcgcgac gtttctcgcc
     2641 agtctgccgc ccgcgccacg cacccgtgac attgcccgag cggttcgttt ccttgcgata
     2701 ccatcctcca ggtaatttgt tacggagtat ggtatgatgc gcgccttttt attgatctaa
     2761 gcacgaagac ccatgcgaat tctggctatc gataccgcga cagaggcctg ctctgtcgcc
     2821 ctgtggaacg acggtactgt caacgctcat tttgagcttt gccctcgtga acatactcaa
     2881 cgaatcttac cgatggtgca ggatatcctg accaccagcg gaacttccct gactgatatt
     2941 aacgctctgg cttacgggcg cggccccggt agctttaccg gcgtgcgcat tggtattggc
     3001 atcgcgcaag ggctggcgct tggcgcggaa ttaccgatga ttggcgtctc cacgctaatg
     3061 acgatggcgc aaggggcgtg gcgcaaaaac ggcgcaacac gcgtgctgtc agccattgac
     3121 gcgcgaatgg gcgaagttta ctgggccgaa tatcagcgtg atgaaaacgg tatctggcac
     3181 ggtgaagaaa ccgaagccgt actcaaaccc gaactcgttc atgaacgaat gcaacagctt
     3241 tccggcgaat gggtgacggt agggacgggc tggcaagcct ggccggatct cggtaaagag
     3301 agcgggctgg ttttgcgcga tggcgaagtg ttactccccg ctgcagaaga tatgctgccg
     3361 attgcttgtc agatgtttgc cgagggtaaa acggtggcgg tggaacatgc cgagccggtt
     3421 tatttgcgta acaacgtcgc atggaagaaa cttccgggca aagaatgaat cttaagagta
     3481 tgccctgaga aaaaaaggag tcgcatcatg gcggttcaaa agaatgttat caaaggcata
     3541 ctggcaggta cgtttgcgct aatgctgagt ggttgtgtca ctgtgccgga cgccattaaa
     3601 ggcagcagtc ccacgccgca acaagattta gttcgggtga tgagtgcgcc gcagctgtac
     3661 gttggtcagg aggcacgctt tggtggcaaa gtggttgcgg tacaaaacca gcaagggaaa
     3721 acccgcctgg aaattgctac cgtaccgctg gacagcggag ctaggccgac gctgggagaa
     3781 ccttctcgcg ggcgcattta tgccgatgtg aacggttttc tggacccggt ggatttccgt
     3841 ggacaactgg ttacggtagt cgggccaatc accggtgcgg ttgacggcaa aatcggcaat
     3901 acgccctata aatttatggt gatgcaagta acgggttaca aacgttggca tttaacccag
     3961 caggtgatta tgccgcctca gccgattgat ccatggtttt atggcggtcg tggctggccc
     4021 tatggctacg gcggatgggg ctggtataat cccggccccg cgagagtaca aacagttgta
     4081 accgaataat tgcttgtttt taaagaaaaa gaaacagcgg ctggtccgct gtttctgcat
     4141 tcttacggta aagataaaaa taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt
     4201 atcaagctgg tatgattagt taatattatg ttaacagcgt gtatatcatt tggggttgcg
     4261 atgacgacga acacgcattt tagaggtgaa gaattgaaga aggtttggct taaccgttat
     4321 cccgcggacg ttccgacgga gatcaaccct gaccgttatc aatctctggt agatatgttt
     4381 gagcagtcgg tcgcgcgcta cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg
     4441 accttccgca agctggaaga acgcagtcgc gcgtttgccg cttatttgca acaagggttg
     4501 gggctgaaga aaggcgatcg cgttgcgttg atgatgccta acttattgca atatccggtg
     4561 gcgctatttg gcattttgcg tgccgggata atcgtcgtaa acgttaaccc gttgtatacc
     4621 ccgcgtgagc ttgagcatca gcttaacgat agcggcgcat cggcgattgt tatcgtgtct
     4681 aactttgccc acactctgga aaaagtggtt gataaaaccg ccgttcagca cgtaattctg
     4741 acccgtatgg gcgatcagct atctacggca aaaggcacgg tagtcaattt cgttgttaaa
     4801 tacatcaagc gcttggtgcc gaaataccat ctgccagatg ccatttcatt tcgtagcgca
     4861 ctgcataacg gctaccggat gcagtacgtc aaacccgaac tggtgccgga agatttagcc
     4921 ttcctgcaat acaccggcgg caccacaggt gtggcgaaag gcgcgatgct gactcaccgc
     4981 aatatgctgg cgaacctgga acaggtcaac gccacctatg ggccgttgct gcatccaggt
     5041 aaagaactgg tagtaacggc actgccgctg tatcacattt ttgccctgac cattaactgc
     5101 ctgctgttta tcgaactggg tgggcagaac ctgcttatca ctaacccgcg tgatattcca
     5161 gggttggtaa aagagttagc gaaatatccg tttaccgcta tcactggcgt taacaccttg
     5221 ttcaatgcgt tgctgaacaa taaagagttc cagcagctgg atttctccag tctgcatctt
     5281 tccgcaggcg gtgggatgcc tgttcagcaa gtggtggcag aacgctgggt gaaactgacc
     5341 ggacagtatc tgctggaagg ttatggcctg accgagtgcg cgccgctggt gagcgttaac
     5401 ccgtatgata ttgattatca tagcggtagc atcggtttgc cggtgccatc gacggaagcc
     5461 aaactggtgg atgacgacga caacgaagta ccaccgggtc aaccgggtga gctttgtgtc
     5521 aaaggaccgc aggtgatgct gggttactgg cagcgtccgg atgctacaga tgagatcatc
     5581 aaaaatggct ggttacacac cggcgacatc gcggtgatgg atgaagaagg attcctgcgc
     5641 attgtcgatc gtaaaaaaga catgattctg gtttccggtt ttaacgtcta tcccaacgag
     5701 attgaagatg tcgtcatgca gcatcctggc gtacaggaag tcgcggctgt tggcgtacct
     5761 tccggctcca gtggtgaagc ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc
     5821 gaagagtcac tggtgacttt ttgccgccgt cagctcacgg gatacaaagt accgaagctg
     5881 gtggagtttc gtgatgagtt accgaaatct aacgtcggaa aaattttgcg acgagaatta
     5941 cgtgacgaag cgcgcggcaa agtggacaat aaagcctgag cgttaagtca gtcgtcagac
     6001 gccggttaat ccggcgtttt ttttgacgcc cactaaagag aaaacaattt gaattaccaa
     6061 atgattacta cggacgatgc gctggcttct ttgtgtgaag ccgtccgtgc ctttccggcg
     6121 atagccctgg atactgaatt tgttcgtacg cgcacttatt acccgcagct ggggttgatt
     6181 caacttttcg atggcgagca tctggcgcta attgatcctc tcgggatcac cgactggtca
     6241 ccgctgaaag cgatcctgcg cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa
     6301 gatctggaag tgttcctcaa tgtctttggc gaattaccac aacccttgat tgacacgcaa
     6361 atccttgctg ccttctgcgg acgcccgatg tcatggggtt tcgcttccat ggtggaagag
     6421 tattccggcg ttacgctgga caagagtgaa tcgcgcgccg actggctggc cagaccgctg
     6481 accgaacgtc agtgtgaata cgcagcggcg gatgtctggt atctgttacc gatcactgcc
     6541 aagcttatgg tagaaacgga ggcctccggc tggctacctg cggcgctgga tgaatgccgc
     6601 ctgatgcaaa tgcgtcgtca ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc
     6661 aatgcctggc agttacgcac acgccaactg gcctgtctgc aactgttagc cgactggcga
     6721 ctgcgcaagg cgcgagagcg tgatctggcg gtgaactttg tcgtgcgtga agagcatttg
     6781 tggtcggtcg cgcgttatat gccgggaagt ttaggcgaac tggacagcct gggtttatcc
     6841 ggtagcgaaa tccgctttca cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca
     6901 ttgccggaag atgccttacc gcagccgatg tttaacctga tggacatgcc tggttatcgt
     6961 aaagcgttta aagcgattaa gtcgctgatt actgacgtga gcgaaacgca taagatcagc
     7021 gccgaattgc tggcatcgcg tcggcaaatc aaccaactgc tgaactggca ccggaaactg
     7081 aaaccgcaga acaatttgcc ggagctgatt tccggctggc gtggtgagct gatggcggaa
     7141 gcattacaca atttattgca ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg
     7201 cgctcagcgc cacatccggc tttggcaaac tacaaatcca acaccaggcg tttacccttc
     7261 gcacgcgaac aacagatcaa catactttgc tggctggcac gctcttcatc gctaaaatac
     7321 tgatcccgat ggtcagcttc accttccagt attgccgttt cgcaggttcc gcatacccct
     7381 tcacgacata aacattccac tttcgcggct ttattatttt caataacctg caaaatagtc
     7441 atctcttccg gcaccacaaa ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg
     7501 ccggttttgt cttcgatagc aaattgctca aagtgcagcg tatcggcggc gatgtccaga
     7561 cgcgcagctt cacttcttac cgtttcaatt agcgcctcgg ggccacaggt ataaacgtgt
     7621 gtgccaggtt cgatatccgc caataatcgc gctaattcca gtcgtgttcc ggttgatgaa
     7681 tgatgcaaat ggactttctc agcctgcgga tgctggacta gctcatcacg aaatgcgcaa
     7741 ctttctggat ttcgcgagca gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct
     7801 gccatgtgcg acaggaaagg ggtgatacca ataccgcccg cgataaacag atgctttctg
     7861 gctgagggaa ttagcgcaaa caggttatta ggcgttgaga tcgttaaccg atcgcccact
     7921 tttacctgct gatgcaaaaa gcgggaaccg ccgcgcgagt tttcttccag tcgaacggca
     7981 atctgataac tagaggtgtt atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg
     8041 ttatcaccat cgctcatctg cactaggatg tgacttcctc cggtgaacgc aggcagcggt
     8101 ttgccatcgg ttgccaccag cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg
     8161 ctcacctgta cttcaaacat ttgatagtct gacattgctg tactccatca aaaatcagcc
     8221 cagcggcgga aaataccgcc gccgatgtca ttagtcctta aacacctgcg ccagcagatt
     8281 atggaaatgg gcgataccat gttcggaaat gccactgccg ctactgtcgg ccatgatgcg
     8341 cccctgacca cgatagccac gcgatttcag ccctttctgt acgctttcaa ccagacgtaa
     8401 atcttccgga tggaacacat cgcgatacca ctcaatcagc gatttttgct cgttggttaa
     8461 ctcttcattg gtgaagtaaa tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa
     8521 ttcataaatg accgtcatca tccctttgat cggggtgacg ttcagcatcg tgcacggcca
     8581 cagccagaaa ccgtggaatg ccgcatccgt accctcttca aatttaaacg actgttcgga
     8641 ctgtttggcg aaaccgtatt gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc
     8701 aacctgtacg gagtcggaga aacctagatg cgccggacca cagtgatagc actcgagata
     8761 gttatcgaca atgttcttcc agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt
     8821 cagattgtgg acttccgggc aggcttccag cactttcgcg cccagaccgg tgtattgatt
     8881 cacttgaagt acgaaaaaaa accgggagga cattggatta ttcgggttct gatgggatta
     8941 gatttggtgg ggcttgcaag cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga
     9001 aagatagtaa aaaattgctt ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat
     9061 catttcaata acagcttcaa aaaacgttca aaaccgataa caaccaggct gtaaccaaat
     9121 gactcatatc acaaatcagc tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg
     9181 tgaagtatgt tttagctggt tcatggttgt tatacggctt tttttacatc ttgtggttct
     9241 tgtgaaggag ctacaagacc ttcctgttca taaataccat actttgacaa aatcttttta
     9301 cgagtatctg ttggtaatga ttcaagcatt ttggctgttt gtaaggtttg ctgcgaaata
     9361 ccttgcaaat cagcctggat ggaatcatcg gaagaaaaaa tctcaggcat aagagcttca
     9421 aatcctttct ttctgaacaa ctcaatcgca gaatgcagtt ccttgtcctc taacaattcg
     9481 acaattgcga tcagtttagt tttcaatgtg ctgcggtcag gttgagaatt tttattctct
     9541 tgattatttt tatgctcaac cattggcatg ctctgacttt ggataaacca ctctattgga
     9601 cgccctgttg cgtaggctat ttgtgctaat cgatctaaag acgggtatgt cttgccagac
     9661 aggtagtttc tgataacccc atcagaaagt ccgcacttag ctgcaaacgc ccggcttgat
     9721 tctccattca ttgcctcttc aaggcgttcc ttgaaacgca ttatttccgg ggcataaaaa
     9781 tgcgctctgc atttttgctt tctttttcac ctgtcatgct caatctctct gttatataaa
     9841 gaattttaca gaattctttg atttgcattg gttaaaataa aaatgtgcat agctcttgaa
     9901 atgcgcattt ttctgcgata tcgtttttcc cgatagcggt agccggtatc agttaccgaa
     9961 aacgttaaga gagtaaggat cgcagaatgt cagcaaaaaa ccaagactgg cacgcagagc
    10021 agataaaggc tgctgttcgt atgaagggga agacactatc tcaattatcc agagaggcag
    10081 gcttaaagcc tgacactatg cgtaacgtat ttcgctgcca tataccacga tacgaagcaa
    10141 tcattgctag atatttggat atggaaccag cccagatttg gccttctcgg tatatggaga
    10201 aaacatcatg ttaacccatt gggtaacagc gcaagaatgc gtaggaatgt catgctttcc
    10261 taaacatgag gtgagtattc gccgcaatct tgaaaagctt gcagccaatc atccagaact
    10321 acgtcgcaag cgtacgggtt caaaggtttt ggaatttcat atatcagttc taccagcggc
    10381 agcacgtgtt gagttgttat taaaatctga gcaaatagaa acaagccagg ggtattttga
    10441 aatcgcccgc cccacgctgg aagcgcacga ttacgatcgt gaggcactgt ggagcaaatg
    10501 ggataaagcc agcgattccc agcgcagact tgctgaaaaa tggttgcctg cggttcagac
    10561 tgcagacgaa atgcttcagc cgttattggt gcgattgtcg tgctatttat ctacaggaag
    10621 attaaaagtt aacgcttaaa ttgcacaaag gctgcacaca ggcagccttt gctatttttt
    10681 agaggtgacg ttaccaccat cccagctcag tgccgagtac gacaataatc gccacaccca
    10741 tcataataat cgttgttttt ttcatcttta tgctcccggg gcagcatagc cgccaataaa
    10801 aaaccatgct aaaaatacga ccgctgtaat gaagattaca accggaaaaa taataccgat
    10861 tcgcatgtta tcaccaatca aaaggatgtg aatacgcctc aggaatgtag cgctggatgc
    10921 gcgacaaaag gtgttttctg tttgtgtttt ttctcctgat acatcgcaat atcggcagca
    10981 tgcagggcac tgtctgcatc ggttgtttca ggatcgactt ctactatgcc aagactggca
    11041 ccgggataat acaaatcaac atcacctaag tgatattctc cacgtatttg ctgttgaata
    11101 cgttctcgca gcgacgaaat atccgcattc tcgttgttta gtgaaacaac caaaaactca
    11161 tcaccaccga gacgaccaat aacttcgcca ttttgctgga gcgtattaag gcgctcgcca
    11221 acctgaatga gaaacagatc gccactatta tgaccaaaac gatcattgat taatttgaag
    11281 ttatccagat cgataaacgc gatcattatc ttatggttaa gatgccgggc gagggaaaac
    11341 agcgtcgtca gattttcaaa aatcgcccgc cgattcggta gcccggttaa cgagtcggta
    11401 tacgattgcg caatcagcgc agcattggct tcgtgcagct gttcaaccag tgcctctttt
    11461 tgaatatatt gtgcaatcag tccggcgaat aactgtaaaa cctgttccgc gcgttcactc
    11521 cactggcgct tctcactgct ggcggcgcaa agcgtgccat agaatgatcc atccggtaag
    11581 tgaattggcg tgctcagaaa tgtggtgatg ccaagattgc gtgccgcaat acagtcgccc
    11641 caacggtcag ggacttcatc gctgaaaaag cagttttcat caatggcgcg tttgcataac
    11701 gagtaatccc acgagacggt aaaattctcc gggatgtgca ttttctggct gttacgggca
    11761 aacataatat gctgcaggcg cgcttcgaca tccactttgg tcaggtaggt tgattccata
    11821 tcagtgacca tttccagcat ctccagaagc tgtcggacca gactttccag tgactgttct
    11881 ttggcaaggg attgcgagac gcgggcgata atctgatctg acacggaaca actccaggat
    11941 acggcgggta agtgctgatt atttaatctt tgcaggcatg ataaaacatc atcaacaaag
    12001 atgaaacaag acgtaatatg ttttgagtga atgtaataaa aatagtgctg caaaacaaaa
    12061 cgataccgcg atactctaag cttggataaa tatgtgagat caataaatga aaattatcag
    12121 ttttgttctg ccttgcttgc tggtcctggc tggttgttca accccttctc agccagaagc
    12181 acctaaaccg ccgcagattg gtatggcaaa tccggcgtcg gtctattgcc agcagaaggg
    12241 cgggacgctc attccagtgc agacagcgca aggggtcagc aacaattgca aattaccggg
    12301 cggtgaaacg attgatgaat gggcattgtg gcgacgggac catccggctg gtgaaaaata
    12361 accgccggat gccgtactaa aatcacaagc tacgtagcca gtcggccagc accagcgcat
    12421 ggttctgcgt ggtgtttttt gctgcgtaga gcagggtcag cggctgtttt ttggcgatgt
    12481 ccgccagccg ctttccttct tgctcgtgtt gcgccagttc tgcgcgatat tgctcgcgaa
    12541 aggttgcaaa atcgacgact tcgccgtgaa aggctttgcg cagttccgtt gacggcgtga
    12601 tttctttatc ccactcatca agggctaaat cggttttttt gataccgcgc ggccagaggc
    12661 ggtcgaccag tacgcgataa ccatcgctct gttcagccgg atcataaacg cgtttgcact
    12721 gaatattcat cgtgtttgtt ctccctttca cgcaagattt gtcacgtcac tatagcaccg
    12781 gatattagcg aatttctttg tctctcccgg cgcaaagtcc gaaaatcgcc atcagtagtg
    12841 aaagtattgc aacacccatc agtggtacgg accagttacc gttagcatcg tgtattttac
    12901 ccattagcgg cggcccacag gctgccagca aatacccgac ggattgtgcc atccccgaga
    12961 gtgccgccgc ctgatgcgca gaacttgccc gaagaccaat gaacgtcaac cccagtatca
    13021 ttgttgcgcc ggaaccaaaa ccgaaaagca gagtccaggt gatcgcgtgc gctggcataa
    13081 agcagagccc aaccgcgccc actgcgcaca tcaaggcaac gaacgctgca ataccacgct
    13141 ggtctttcac atgatgtaag aaaagtggga tcagcaaacc gggagctgct gtggctagtt
    13201 gcagcaaacc atgcagtgaa cccgcctgtg cttcgctata gccgtgactg atgaggatcg
    13261 ccggaagcca gccaataatc acgtaataga ccagtgagtt gatcccaaga aacaatgtta
    13321 cctgctaggc aagtggcgaa cgccagatac cccgagtatg taaggcgcgt gaggtactca
    13381 aatttgcatg ttgctgactt cgccactgtg gcagccataa aaaaagagcc agcagaggaa
    13441 aacacatcag catgaacaac gcgccttgcc agccaaaacc gttcaaagcc agcggcacga
    13501 ccatagccga tcccagtgcc gctgcagctc ccattgtcag ggaatatgcg ccggtaagtc
    13561 tggcgacgga atgagggaaa tcgcgtttaa ttaatcctgg cagtaagaca ttgcctaatg
    13621 caatcccacc gccaatgacc gctgtaccgc caaataataa gtaaggcgaa gggagagaac
    13681 gaattgcgat acctgcacag atcaaaagta acgcggcaaa caggctacgt tccataccaa
    13741 aacgtcgcgc caccggggca gccagaggtg agattagcgc aaaggccaat aatggcaggg
    13801 tggttaataa gccggtttgc gctgtcgtca gcgagtaagc ggaacgaatc gtatccagta
    13861 acggcgctgc gccggtaaag gtgacgcgta atgttgtggc aatcatcaga atgccagcga
    13921 taaggacaat cctgtttttg ccgcttaatg aagttgaaca ggtcatatgc ttctcattgt
    13981 tacaaacgag gctgtacctt accgttttta ttgttatgtt gaatcaagct aaaatgacaa
    14041 attatcgcta agttcggaca aaatgatgca caggttgaat ctcaatggct atgaacccga
    14101 ccgacaccat gaagccgcgg tcgcattttg tattcacgcc ggaacggatg agttaaccag
    14161 tcctgtacat cagcaccgca aaggacagct gatactagca ttacatggtg caattacctg
    14221 tacggtggaa aatgctttgt ggatggtgcc gccacaatat gcggtttgga taccgggcgg
    14281 ggtgaagcat agcaatcaag tgacagctaa cgctgaactc tgttttctgt ttatcgaacc
    14341 cagcgcagtg acaatgccga caacatgttg cacattgaaa atatcgccct tatgccggga
    14401 attaatttta accctggcaa acaggacgac gactcagcgt gctgagccga tgacccgcag
    14461 attaattcag gtcctgtttg atgaactccc tcagcagcca caacaacagc tgcacctgcc
    14521 cgtttcttct catcctaaaa tccgcacgat ggtggagatg atggcgaaag ggcccgtcga
    14581 gtgggggtca ttggggcaat gggctggctt ttttgcgatg agtgaacgca atcttgcccg
    14641 actgatagtg aaagagacgg ggttgagttt tcgccagtgg cgtcagcagt tacagctgat
    14701 tatggcgctt cagggattag tgaaaggcga tactgtacag aaagttgctc atacgctggg
    14761 gtatgattca acaacggcct ttattactat gtttaagaaa ggcctagggc aaacgccagg
    14821 ccgatatatt gcgggattaa ctactgtttc cccacaatca gcgaaaccag acccgcggca
    14881 ataagcgggc caaccggtac gccgcgaaac agcgctacgc ctaaaacagt gcccaccagc
    14941 agcccagcaa ccagttgcgg ctggctgccc attaacgtca cgccgcgccc acccagccag
    15001 gagacaataa cgcctactgc aattgccacc agcgatttcc agttcagaaa cgagtgaatc
    15061 aaggttgagg gcggtagcgt cccgctggcg atgggggcca taacgccaat cgtcagaatg
    15121 attatcccga tacttagccc ctgtttttca atccacggga aaaaggtgct taacggtgtg
    15181 acgcggacaa tgatcaacac cagaattgaa acggcgacag tggtgttatg actgataaag
    15241 cccagcgcag ctaacccgag caggatcagc aaagtgacat caaacatcgt ggtttccttg
    15301 ccaaaaaata ataagcctgc ttactttacg cttgctgcag gaaggaagta gaaaatcttt
    15361 acgttttttg cataaaaaat caggcgaact gtcatcaaac tgccatttgt gcacgctaac
    15421 aataacgtga aaacagctat ctcccggggg catgctatga acgatcaaat gttcgtcgag
    15481 acactgatta tcacatcatc gttttttgct attgcggttg tgctggtttt gtccgttctg
    15541 cttattgaac ggataggcta gctacgcagt gcggcggaaa ttgaccagtt ctggttgcgc
    15601 aatgcgcaaa taatccgcgg ttttcaaaat aacggattta tccagcatcc ctgtattgaa
    15661 ggcgatttca tcaaatcgtt cgaagagtag agggtcggca accagtttga gttttggatg
    15721 gaagctgaaa ggggggatcg cgccgaagac acagcctgtg agttcatcaa cttccgccgg
    15781 gctggccaac gaagcgcgta atccgcctat atggctggcg agttggctca gatcggcctg
    15841 ttgatcggct gcgaggatcg ccagaacatg ttgatttacg ccattgcctt tgactttaca
    15901 taccagtgcc ttagcaccct ggcctagagc ggtgccacga atttctgaca ccgcttcaca
    15961 tttacctacc gcttcatggg taacgacgcg gaagttagca ccttcttgtg ataataaagc
    16021 gattaactgc tgatgcgtca cgcttccttt agccatttca gtcatttcac gctcctgaga
    16081 ttacaagcaa acaaccacag aaggttatca tgaacggctg tttttgttct gcttattgac
    16141 atataaacgc tcatcggaag ctttatacgc atcatgtaac gtaatcgcta aatccccctc
    16201 ctgatgaccg aggttgtcat ttatttgctt taacttgtcc atgtcaattg caataaacat
    16261 caccgaagaa ccggattgca ccagtttctg caaccgctgc tccagttcag gggttaaaat
    16321 tttgcgatta tacagtccag tcatcgcatc actaatattt tctcgcgaca cattttggta
    16381 taaacggaag tgcatccgga ccatattcag caaaattaac gccgtcaaaa tccagaacag
    16441 aacgcttttc cacgaagacg tgataaagta aagaatatca atggataacg agacacgaat
    16501 gccgcccggt aagtcatgga cgtaactgac atactggaac agattatctt cgctctggtt
    16561 gatgataatg tcgcgccccg aatcggtatc ggttagcgtg acattgagaa aacgccagag
    16621 gagagggcgg tcatgagtgt aaaagatatt ccgcaaattg tttttgttaa tatccagcag
    16681 cacaatccct tttagctgcc ctgcgacata taccggcgtc agcaaactca tcacgtgctg
    16741 tttagtacgc tgatcctggt aaatgctcga caatacggta cgcccggtaa ataccttttc
    16801 aatatctttg cgatcgatgc caatacttcc tgactgcagg aaggaccagt tattcatcgc
    16861 aaagtattca acattaaccg gctcataaaa atagacatag cggtcattga gatcgagata
    16921 atagtgaaat ttctcttcgg ctatcccaag acctgaaccg taatcatcct ggtggcggtt
    16981 gccttccact gcctgatcaa aagcgggtaa tagtgctgca gcttccacaa ttgtgtcgca
    17041 gtcagtggat gcggtttgta gtgtgccgcg taagcccggg tatttatggg cagtcaggtt
    17101 taagccatgg gtattattgg cagactcaaa agcctcgcag atggcgcgcc gctgttctgg
    17161 cgacacttcc gactgctcag cagcgtgagg tagcctgagt ttaacggaca ctccttcctg
    17221 aaatagaatg gcatcagaag gagctaataa tgagcagaaa aacccaacgt tactctaaag
    17281 agttcaaagc cgaagctgtc agaacggttc ttgaaaatca actttcgatc agtgaaggcg
    17341 cttcccgatt atctcttcct gaaggcactt taggacaat
//
LOCUS       DAETPZ010000078        17373 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.078, whole genome shotgun sequence.
ACCESSION   DAETPZ010000078 DAETPZ010000000
VERSION     DAETPZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.078"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..600)
                     /locus_tag="K0W59_002994"
     CDS             complement(<1..600)
                     /locus_tag="K0W59_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBI3023943.1"
                     /translation="MLAKDKSNLKIEEIRMHKHHEIHKVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIVNYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPRTKWTANKVARYLYISVSTLHRRLA"
     gene            complement(610..1161)
                     /gene="ybcL"
                     /locus_tag="K0W59_002995"
     CDS             complement(610..1161)
                     /gene="ybcL"
                     /locus_tag="K0W59_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HBI3023944.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(1330..1662)
                     /gene="emrE"
                     /locus_tag="K0W59_002996"
     CDS             complement(1330..1662)
                     /gene="emrE"
                     /locus_tag="K0W59_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HBI3023945.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2007..2321)
                     /gene="fliE"
                     /locus_tag="K0W59_002997"
     CDS             complement(2007..2321)
                     /gene="fliE"
                     /locus_tag="K0W59_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBI3023946.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAAIDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2535..4193
                     /gene="fliF"
                     /locus_tag="K0W59_002998"
                     /pseudo
     CDS             2535..4193
                     /gene="fliF"
                     /locus_tag="K0W59_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            4186..5181
                     /gene="fliG"
                     /locus_tag="K0W59_002999"
     CDS             4186..5181
                     /gene="fliG"
                     /locus_tag="K0W59_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBI3023947.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVNDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5174..5860
                     /gene="fliH"
                     /locus_tag="K0W59_003000"
     CDS             5174..5860
                     /gene="fliH"
                     /locus_tag="K0W59_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBI3023948.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQVHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAALGVV"
     gene            5860..7233
                     /gene="fliI"
                     /locus_tag="K0W59_003001"
     CDS             5860..7233
                     /gene="fliI"
                     /locus_tag="K0W59_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HBI3023949.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7252..7695
                     /gene="fliJ"
                     /locus_tag="K0W59_003002"
     CDS             7252..7695
                     /gene="fliJ"
                     /locus_tag="K0W59_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HBI3023950.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7692..8819
                     /gene="fliK"
                     /locus_tag="K0W59_003003"
     CDS             7692..8819
                     /gene="fliK"
                     /locus_tag="K0W59_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HBI3023951.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8924..9388
                     /gene="fliL"
                     /locus_tag="K0W59_003004"
     CDS             8924..9388
                     /gene="fliL"
                     /locus_tag="K0W59_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HBI3023952.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            9393..10397
                     /gene="fliM"
                     /locus_tag="K0W59_003005"
     CDS             9393..10397
                     /gene="fliM"
                     /locus_tag="K0W59_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HBI3023953.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10394..10807
                     /gene="fliN"
                     /locus_tag="K0W59_003006"
     CDS             10394..10807
                     /gene="fliN"
                     /locus_tag="K0W59_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HBI3023954.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILNN
                     GYLIAQGEVVVVADKYDVRITDIITPSERMRRLSR"
     gene            10810..11175
                     /gene="fliO"
                     /locus_tag="K0W59_003007"
     CDS             10810..11175
                     /gene="fliO"
                     /locus_tag="K0W59_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HBI3023955.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            11175..11912
                     /gene="fliP"
                     /locus_tag="K0W59_003008"
     CDS             11175..11912
                     /gene="fliP"
                     /locus_tag="K0W59_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBI3023956.1"
                     /translation="MRRLFSVAPVLLWLIMPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11922..12191
                     /gene="fliQ"
                     /locus_tag="K0W59_003009"
     CDS             11922..12191
                     /gene="fliQ"
                     /locus_tag="K0W59_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBI3023957.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12200..12984
                     /gene="fliR"
                     /locus_tag="K0W59_003010"
                     /pseudo
     CDS             12200..12984
                     /gene="fliR"
                     /locus_tag="K0W59_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
     gene            13269..13892
                     /gene="rcsA"
                     /locus_tag="K0W59_003011"
     CDS             13269..13892
                     /gene="rcsA"
                     /locus_tag="K0W59_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HBI3023958.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(13936..14124)
                     /gene="dsrB"
                     /locus_tag="K0W59_003012"
     CDS             complement(13936..14124)
                     /gene="dsrB"
                     /locus_tag="K0W59_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HBI3023959.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            14287..14514
                     /gene="yodD"
                     /locus_tag="K0W59_003013"
     CDS             14287..14514
                     /gene="yodD"
                     /locus_tag="K0W59_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HBI3023960.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            14812..15627
                     /locus_tag="K0W59_003014"
                     /pseudo
     CDS             14812..15627
                     /locus_tag="K0W59_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(15624..17318)
                     /gene="yedQ"
                     /locus_tag="K0W59_003015"
     CDS             complement(15624..17318)
                     /gene="yedQ"
                     /locus_tag="K0W59_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HBI3023961.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNFLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFMRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLIFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGCIRMDSRYVSWERL
                     DHFDGVLARVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
BASE COUNT         4372 a         4413 c         4470 g         4118 t
ORIGIN      
        1 tgccagacgg cgatgcaatg tggaaacaga aatgtagaga tatctcgcta ccttgtttgc
       61 tgtccatttt gttctaggtt cggatagcat taggttataa caacgactta ttaatgattg
      121 tttactatct gataaaagta aacaattaac atgatgtgct cctaatgaaa gtagaacgcc
      181 cattgctaag tgttcctgaa tttgcgttga gaagcctcgg gaaatagatg tttttagttg
      241 ctcccaacaa tatattaact cggggttctg aggtaagcag aaacctgtcg tgttacgaat
      301 ttgatcagtt atcgcataaa atttttgaaa cttttcaatt agatcaatgg gataataaag
      361 catttctgca agataaagac ctgcttctgg ataattcaca atataaaatt catatccaca
      421 gggaaataat attatctgat tattatcaac agttagacta tgcgtctccc aattgataac
      481 tttttttccc tgacggatac gacataaagc tggcataaga ggcttaacct tgtgaatctc
      541 atgatgttta tgcatccgta tttcttcaat ctttaggtta gacttatctt ttgccagcat
      601 actcttaccc tactttatct cataaactgg tgttatctca gcggttgcaa ttttattggc
      661 attaagcata taaccaacta acgctccgct ggaattagaa tccacaggaa tcttatcagt
      721 ttttagagcc catactttaa actggtaatg atggggttta tctcctttgg gaggacatgc
      781 tccaccaaat cctgcataac caaaatcatt tcggccttga acagcaccag tcggaagttt
      841 tgtaccatca cgtcttcctg catcagtggg caaatatgtt acagttgctg gaatattagc
      901 aacagtccag tgccaccatc cactgcctgt aggagcatct ggatcataca cagttacggc
      961 aaagcttttg gtgccttcag gagcaccaga ccaggttaat gagggcgatg tattaccacc
     1021 ttcacaccca aatccagaaa atacatgaga cgttgtaagt tgctctcctg tttttatttc
     1081 attactagtg acctgaaatg ctgcggcctg cgcagaaaat gttatgaatg ccaatacact
     1141 tgaaacgata agttttttca taaaaacctc attgttgtga cctatcgtta ttttatttga
     1201 tgtgccttga tctcattatg cataaaggca caatgatcgc gcaaatgcaa tcataattat
     1261 aaaggtgccc cgaacattag ccataatatg cacaaatcag attcctgtta tctcaaggag
     1321 aattattttt taatgcggtg cacttcgtga caacaagtta atgactaaca caccggcaca
     1381 aatcaacatc atgcctatga tagctggcag gtccagtcgt tgaccaaaaa atccccagga
     1441 aagcaaacta atcagaacga taccgactcc tgaccagata gcataagcaa tccctgtagg
     1501 tatataagcc agcgtctgag ctaataacca gaatgatgca caatagcaaa taattgtgcc
     1561 aacagagggc cataaccgtg taaacccctc tgaaaacttc attaaggttg taccaatgac
     1621 ctctgcaagt attgcgccac caagataaat ataagggttc atagcatatt ctttcctgtt
     1681 catactggag agaattgtac tacagtttga acacaactca ccgttttcat cctagtgtat
     1741 gtgtatccaa ggggctctgg cgtggcaaga gataaaggca ggaacaataa tgatcagaag
     1801 gaaccccagg ctaacgaatg agttcactct tcctgggagc aaagcgaaga caaaacatgt
     1861 tatcgcgttt acggatcgtg gatggcagaa aacaaccagg atcaggtatc cattttaaac
     1921 cagaaattga gtgagtttgc cccatccatg ccccacgctg tgggatcgga tataattaaa
     1981 caagcatagt caacaggtta cacaacctac acctgcatag acatcacttc ctgatacgcc
     2041 gccaccagct tattacgcac ctgaatcccc atttgcatag aaactgaggc tttttgcata
     2101 tcggtcatca catcgtttag cgccacgccg ggttcaccga gggtgaattt ttctgcctgc
     2161 gtgcgggcga ctgtttgtgt atcgcttatg cgatcgatcg cggcgtgcag ctgcccggca
     2221 aaactaatgg tcggttgcgg cagtgattcc tgcgcacgcg cactcatcgc cgtagcctgt
     2281 aactggctga taaccccttc aatcccctgt atcgctgaca ttttcgtctc ccggataatt
     2341 tctggtaaca aagcctacca gtaagtcaat aagacaaagg cgctaaatag caacaaaaaa
     2401 acgggtttat tggcggatag aaaaaacgaa agcacaaata atgggagcgt caatttttcg
     2461 agtttgctga cccgagagtg agtcttgttc cactttgcca ataacgccgt ccataatcag
     2521 ccacgaggtg cgcgatgaat gcgactgcag cccagacaaa atctcttgag tggcttaatc
     2581 gcctgcgtgc gaatccgaaa attccattga ttgttaccgg ttccgcggca gtggcggtca
     2641 tggtcgcact gatcctgtgg gcgaaagccc ccgactaccg cacattattc agcaatcttt
     2701 ccgatcagga tggtggcgca attgtcagcc aactgacgca aatgaatatt ccttaccgct
     2761 tcagcgaagc cagcggcgct attgaggttc cggcagataa agttcacgaa ctgcgcctgc
     2821 gcctggcgca acaaggtttg cccaaaggcg gcgcggtcgg tttcgaactg ctcgatcagg
     2881 aaaagtttgg tatcagccag ttcagcgaac aggtgaatta tcagcgggcg ctggaaggcg
     2941 agctttctcg taccatcgaa actatcggcc cggtaaaagg ggcgcgcgta catctggcaa
     3001 tgccgaaacc gtctttattc gtccgtgaac aaaaatcccc ttctgcatcg gtgacggtaa
     3061 atctgttacc cggccgcgca ctcgatgaag ggcaaattag cgctattgta catctggttt
     3121 ccagcgcggt tgctggtctg ccgcctggaa acgtcacgct ggtggatcag ggcgggcatc
     3181 tgttaaccca gtccaatacc agcgggcgcg atcttaatga cgctcagttg aaatatgcca
     3241 gcgatgtcga aggccgtatt cagcggcgta ttgaagcgat cctgtcgcct attgttggta
     3301 acggtaatat tcacgcctag gtcacggcgc aactggactt cgccagtaaa gaacaaacgg
     3361 aagaacagta tcgccctaac ggtgatgaat ctcatgcggc gcttcgttca cgccagctta
     3421 atgagagcga gcaaagcggt tccggttatc cgggcggcgt accgggggcg ttgtcgaatc
     3481 aaccggcacc tgcgaataac gcgccaatca gcacgcctcc gacaaatcaa aataaccgcc
     3541 agcagcaggc gagcaccacc agcaatagtg ggccgcgtag cacacagcgg aatgaaacca
     3601 gtaactacga agtcgatcgc accattcgtc ataccaaaat gaacgtgggc gatgtgcaac
     3661 gtctgtcagt cgcggtagtg gtgaattaca aaaccttgcc agacggtaaa ccattgcctc
     3721 tcagcaacga acagatgaag caaattgaag ctctgacccg cgaggcgatg ggcttttctg
     3781 aaaaacgcgg tgattcgctc aatgttgtta actcgccgtt caatagcagt gacgaaagcg
     3841 gcggagcact gccgttttgg caacagcaag tgtttatcga tcagttgctg gctgccggtc
     3901 gctggttgct ggtactgctg gtggcgtggt tgctgtggcg gaaagcggta cgcccgcagc
     3961 taacacgtcg cgccgaggcg gtgaagactg tgcagcaaca ggcgcaggcc cgcgaggaag
     4021 tggaagatgc ggtggaagtc cgtctgagca aagacgaaca actccaacaa cggcgcgcta
     4081 accaacgact gggggcagaa gtcatgagcc agcgtatccg tgaaatgtct gataacgatc
     4141 cgcgcgtggt ggcgctggtc attcgccagt ggataaataa cgatcatgag taacctgaca
     4201 ggcaccgata aaagcgtcat cctgctgatg accattggcg aagaccgggc ggcagaggtg
     4261 ttcaagcacc tctcccagcg cgaagtgcaa accctgagcg ctgcaatggc gaacgtcacg
     4321 cagatctcca acaagcagct aaccgatgtg ctggcggagt ttgagcaaga agctgaacag
     4381 tttgctgcac tgaatatcaa cgccaacgat tatctgcgtt cggtattggt caaagctctg
     4441 ggtgaagaac gtgccgccag cctgctggaa gatattctcg aaacccgcga taccgccagc
     4501 ggtattgaaa cgctcaactt tatggagccg cagagcgccg ccgatctgat tcgcgatgag
     4561 catccgcaaa ttatcgccac cattctggtg catctgaagc gcgcccaggc cgccgatatt
     4621 ctggcgttgt tcgatgaacg tctgcgccac gacgtgatgt tgcgtatcgc cacctttggc
     4681 ggcgtgcagc cagccgcgct ggccgaactg accgaagtac tgaatggctt gctcgacggt
     4741 cagaatctca agcgcagcaa aatgggcggc gtgagaacgg cagccgaaat tatcaacctg
     4801 atgaaaacgc agcaggaaga agccgttatt accgccgtgc gtgaattcga cggcgagctg
     4861 gcgcagaaaa tcatcgacga gatgttcctg ttcgagaatc tggtggatgt caatgatcgc
     4921 agcattcagc gtctgttgca ggaagtggat tccgaatcgc tgctgatcgc gctgaaaggg
     4981 gccgagcagc cactgcgcga gaaattcctg cgcaatatgt cgcagcgtgc cgccgatatt
     5041 ctgcgcgacg atctcgccaa ccgtggtccg gtgcgtctgt cgcaggtcga aaacgaacag
     5101 aaagcgattc tgctgattgt gcgccgtctt gccgaaactg gcgagatggt gattggcagc
     5161 ggcgaggata cctatgtctg ataatctgcc gtggaaaacc tggacgccgg acgatctcgc
     5221 gccaccaccg gcagagtttg tgccaatggt cgagtcggaa gaaaccatta ttgaagaggc
     5281 cgaacccagc cttgagcagc aactggcgca actgcaaatg caggtccacg agcagggtta
     5341 tcaggcgggg attgccgaag gtcgccagca aggtcacgaa cagggctatc aggaaggact
     5401 ggcccagggg ctggaacaag gtctggcaga ggcgaaggct caacaagcgc caattcatgc
     5461 ccggatgcag caactggtca gcgaatttca aactaccctt gatgcgcttg atagtgtgat
     5521 agcttcgcgc ctgatgcaga tggcgctgga ggcagcacgt caggtgatcg gtcagacacc
     5581 aacggtggat aactcggcac tgatcaaaca gatccaacag ttgttgcagc aagaaccgtt
     5641 attcagcggg aaaccacagc tgcgcgtaca cccggatgat ctgcaacgtg tggatgatat
     5701 gctcggcgca actttaagtt tgcatggctg gcgcttgcgg ggcgatccca ccctccaccc
     5761 tggcggatgt aaagtctccg ccgatgaagg cgatctcgac gccagtgtcg ccactcgctg
     5821 gcaagaactc tgccgtctgg cagcactagg agtggtgtaa tgaccacgcg cctgactcga
     5881 tggctaacca cgctggataa ctttgaagcc aaaatggcgc agttgcctgc ggtacgtcgc
     5941 tacgggcgat taacccgcgc taccgggctg gtgctggaag ccaccggatt acaattgccg
     6001 ctcggcgcaa cctgtgtcat tgagcgccag aacggcagcg aaacgcacga agtagaaagc
     6061 gaagtcgttg gctttaacgg tcaacggctg tttttaatgc cgctggagga agtggaaggt
     6121 gtcctgcccg gcgcgcgtgt atatgccaaa aacatttcgg cagaagggct gcaaagcggc
     6181 aagcagttgc cgctcggtcc ggcgttatta ggtcgtgttc tggacggtag cggtaaaccg
     6241 ctcgatggcc tgcccttccc cgatacgacg gaaaccggtg cgctgattac cccgccattt
     6301 aacccgttgc aacgtacacc gattgaacat gtgctggaca ccggcgtgcg cccaatcaat
     6361 gccctgctta ccgtcggacg cgggcagcgt atggggctgt ttgccgggtc gggcgttggt
     6421 aaaagtgtgc tgctggggat gatggcacgt tacacccgcg ccgatgtcat tgtcgtgggt
     6481 ttgattggtg aacgtgggcg cgaagtaaaa gattttattg agaatatcct cggtgccgaa
     6541 ggacgtgcac gctcagtggt gattgccgct ccggcggatg tttctccgct cctgcgaatg
     6601 caaggtgccg cctatgccac gcgcattgcc gaagattttc gcgatcgtgg tcagcatgtg
     6661 ttgctgatta tggactccct cacccgctac gcgatggccc agcgtgaaat tgcgctggcg
     6721 attggcgaac cacctgccac taaaggttat ccaccgtcgg tgtttgccaa attaccggca
     6781 ctggtcgagc gtgccggtaa tggcattagc ggcggcggct cgattaccgc gttttatacc
     6841 gtgctcaccg aaggcgatga ccagcaggac cccattgctg actccgcgcg ggcaatcctc
     6901 gacggtcaca ttgtgctgtc tcgccgactg gcggaagccg ggcactatcc ggctatcgat
     6961 attgaagcgt cgatcagtcg cgcaatgacg gcgttgatca gtgagcaaca ttacgcgcga
     7021 gtgcgcacct tcaaacagct gttgtcgagt tttcagcgta accgcgatct ggttagcgtc
     7081 ggcgcgtatg ccaaaggcag cgatccgatg ctcgataaag ccatcgccct gtggccgcag
     7141 ctggagggct atttgcaaca aggcattttt gaacgcgcgg actgggaagc gtctctccag
     7201 gggctggagc gtattttccc gacagtgtca taacccagga gataacggca gatggcggaa
     7261 catggtgcgc tggcgaccct gaaagatctg gcagaaaaag aggtagagga tgccgctcgc
     7321 ctgctgggtg aaatgcgtcg cggatgtcag caggcggaag aacagctcaa aatgctgatc
     7381 gattatcaga atgaatatcg caataacctc aatagcgata tgagtgccgg gatgaccagc
     7441 aaccgctgga tcaactatca gcagtttatc cagacgctgg aaaaagccat tactcagcat
     7501 cgccagcaac ttaatcagtg gacgcagaaa gttgacattg ccctgaacag ctggcgagaa
     7561 aagaaacaac gtttgcaggc ctggcagaca ctgcaggaac ggcaatccac ggcggcactg
     7621 cttgcagaaa accgcctcga tcagaaaaag atggatgagt tcgcccagcg cgccgccatg
     7681 aggaaacctg aatgattcgc ttagcgccct tgattaccgc cgacgttgac accaccacat
     7741 tgcctggcgg caaagccagc gatgctgcac aagattttct cacgttgttg agcgaagcat
     7801 tagcaggcga gacaacaact gacaaagcgg ccccccagtt gctggtggca acagataagc
     7861 ccacgacaaa aggcgagccg ctggtcagcg atattcttgc cgatgcgcaa caagcggatt
     7921 tactgatccc tgtggatgaa acacttcctg tcatcaacga cgaacaatcc acatcaacac
     7981 cgttaaccac cgctcagaca atgacgttgg ctgcggtggc tgacaaaaat acgacaaaag
     8041 acgaaaaagc ggatgatctt aatgaagacg tcaccgcaag tctgagcgcc ctttttgcga
     8101 tgttgccggg ttttgacaat acgcccaaag tgactgatgc accgtcaacc gtgttaccgg
     8161 cagagaaacc aacgctcttc acaaaactga cttctgcgca actcactaca gcacagcctg
     8221 atgacgcccc cggcacacca gctcagccat taacaccgct ggtagcagaa gcccagagta
     8281 aagcggaagt catcagcacg ccttcgccgg tgaccgctga cgccagcccg ctaatcactc
     8341 cacaccagac acagccactg cccaccgtcg ccgcgcctgt tttgagtgca ccgctgggtt
     8401 ctcacgaatg gcaacaatca ttgagccagc atatttcgct gttcacccgc caggggcaac
     8461 aaagtgcaga gttgcgtctg cacccacagg atttaggtga agtgcaaatt tccctcaaag
     8521 tggatgataa ccaggcgcag atccagatga tttcaccgca tcagcacgta cgcgccgccc
     8581 tggaagcagc gctgccggta ctgcgtacgc agctggccga aagtggcatt cagttagggc
     8641 aaagcaacat tagtggcgaa agctttagtg gtcagcagca ggccgcttcc cagcaacagc
     8701 aaagccaacg cacagcaaac catgaacctc tggcggggga agacgacgat acgcttccgg
     8761 ttcccgtctc tttacaaggg cgtgtaacag gtaacagcgg cgttgatatt ttcgcctaac
     8821 gtcagaggta gcaccgtaat ccgcgtcttt tccccgcttt gttgcgctca agacgcagga
     8881 taattagccg ataagcagta gcgacacagg aagaccgcaa cacatgactg attacgcgat
     8941 aagcaagaaa agcaagcgat cgctttggat cccgattctg gtattcatta ccctcgcggc
     9001 ctgtgccagc gcaggttaca gctactggca ttcgcatcag gttgccgctg acgacaaagc
     9061 gcagcaacgc gtcgtgccct ccccggtctt ctatgcgctg gataccttta cggtcaattt
     9121 gggcgatgcg gatcgcgtgc tttatatcgg cataaccctg cgactgaaag atgaagcaac
     9181 ccgctcgcgg ctgagtgagt atttgccgga agtccgtagt cgcttgctgt tactgttttc
     9241 acgtcaggat gctgccgtac tggcgacaga agaaggcaag aaaaacctga ttgccgagat
     9301 taaaaccaca ctttccaccc cgcttgttgc cgggcaaccg aaacaggatg tcaccgacgt
     9361 gctgtatacc gcttttattc tgcgataacg acatgggcga tagtattctt tctcaagctg
     9421 aaattgatgc gctgttgaat ggtgacagcg aagtcaaaga cgaaccgaca gccagtgtta
     9481 gcggcgaaag tgacattcgt ccgtacgatc cgaataccca acgacgggtt gtgcgcgaac
     9541 gtttgcaggc gctggaaatc attaatgagc gctttgcccg ccattttcgt atggggctgt
     9601 tcaacctgct gcgccgtagc ccggacatta ccgtcggggc catccgcatt cagccatacc
     9661 atgaatttgc ccgcaacctg ccggtgccga ccaacctgaa cctaatccac ctgaaaccgc
     9721 tacgcggcac agggctggta gtgttctcgc cgagtctggt gtttatcgcc gtggataacc
     9781 tgtttggcgg cgatggacgc ttcccgacca aagtggaagg tcgcgagttt acccatactg
     9841 aacagcgcgt catcaaccgt atgttgaagc tggcgctgga aggctatagc gacgcctgga
     9901 aagcgattaa tccgctggaa gttgagtacg tgcgttcgga aatgcaggtg gaatttacca
     9961 atatcaccac ctcgccgaac gacattgtgg ttaacacgcc gttccatgtg gagattggca
    10021 acctgaccgg cgaatttaat atctgcctgc cattcagcat gatcgagccg ctacgggaat
    10081 tgttggttaa cccgccgctg gaaaactcgc gtaatgaaga tcagaactgg cgcgataacc
    10141 tggtgcgcca ggtgcagcat tcacagctgg aactggtggc caactttgcc gatatctcgc
    10201 tacgcctgtc gcagatttta aaactgaaac ccggcgacgt cctgccgata gaaaaacccg
    10261 atcgcatcat cgcccatgtt gacggcgtcc cggttctgac cagtcagtac ggcactctca
    10321 acggtcagta tgcgttacgg atagaacatt tgattaaccc gattttaaat tctctgaacg
    10381 aggaacagcc caaatgagtg acatgaataa tccggccgat gacaacaacg gcgcaatgga
    10441 cgatctgtgg gctgaagcgt tgagcgagca aaaatcaacc agcgaaaaaa gcgctgccga
    10501 cgcggtgttc cagcaatttg gcggtggtga tgtcagcgga acgttgcagg atatcgacct
    10561 gattatggat attccggtca agctgaccgt cgagctgggc cgtacgcgga tgaccatcaa
    10621 agagctgttg cgtctgacgc aagggtcagt cgtggcgctg gacggtctgg cgggcgaacc
    10681 gctggatatt ctgaacaacg gttatttaat cgcccagggc gaagtggtgg ttgttgccga
    10741 taaatatgac gtgcggatca ccgatatcat taccccgtcc gagcggatgc gccgcctgag
    10801 ccgttagtga tgaataacca tgctactgtg caatcttccg cgccggtttc tgctgagcca
    10861 ctgctgcagg tgagcggcgc actcatcgcc attattgccc tgatcctcgc tgctgcctgg
    10921 ctggtaaaac ggctgggatt tgcccctaaa cgcactggcg ttaacggtct gaaaattagc
    10981 gccagtgctt cgctgggcgc gcgtgaacgg gtcgtggtgg tcgatgtgga agatgcacgg
    11041 ctggtgctcg gcgttaccgc agggcaaatc aatctgctgc ataaacttcc cccttcagca
    11101 ccaacggaag agataccgca gaccgatttt cagtcggtca tgaaaaattt gcttaagcgt
    11161 ggcgggagat cctgatgcgt cgtttgtttt ctgtcgcacc ggtccttctc tggctgatta
    11221 tgcccctcgc cttcgcgcaa ctgccgggta tcaccagcca gccgctacct ggcggtggac
    11281 aaagctggtc gctcccggtg cagacgctgg tgttcatcac ctcgttgacg ttcattccag
    11341 cgattttact gatgatgacc agtttcaccc gcatcattat tgtttttggt ttattgcgta
    11401 acgcgctggg aacgccctcc gcgccaccta accaggtatt gctggggctg gcactgtttt
    11461 tgaccttttt tattatgtcg ccagtgatcg acaaaattta tgtcgatgcg taccagccat
    11521 tcagcgaaga gaaaatctca atgcaggagg cgctggaaaa aggggcgcag ccgctgcgtg
    11581 agtttatgct gcgtcagacc cgcgaggcag atttagggtt gtttgccaga ctggcgaata
    11641 ccggcccgtt gcaggggcct gaagctgtac cgatgcgcat tttgctcccg gcctacgtga
    11701 ccagcgagtt gaaaaccgca tttcagatag gcttcacgat tttcatccct tttttgatta
    11761 tcgacctggt gattgccagc gtgttgatgg cattggggat gatgatggtt ccccccgcca
    11821 ccattgctct gccctttaaa ctgatgctgt ttgtactggt ggatggctgg caattgctgg
    11881 tcggttcgct ggcgcagagc ttttacagct agagaggcaa aatgacacct gaatcggtca
    11941 tgatgatggg aactgaagcg atgaaagtcg cgctggcact ggcagccccg ctgttgctgg
    12001 tagcgctggt cacgggcctt atcatcagta ttttgcaggc agccacgcag attaacgaaa
    12061 tgacgctgtc gtttattccg aaaatcatcg ccgtatttat cgccatcatt attgccggac
    12121 cgtggatgct caatctgttg ctggattacg tccgcacctt gttcactaac ctgccgtata
    12181 tcatcgggta gcccgtacaa tgatgcagga gacaagcgat caatggcttt cctggttaag
    12241 cctgtacttc tggccgttac tgcgcgtgct ggcgctcatt tctaccgcgc caattctgag
    12301 cgaacgcagc gtgccgaaac gggtaaaact gggtctggca atgatgatca cgttcgccat
    12361 tgccccatca ttacctgcca acgatgttcc tgttttttcg ttctttgctc tgtggctggc
    12421 cgtgcagcag atcctgatcg gcattgcgct tggttttacc atgcaatttg cctttgccgc
    12481 tgtgcgaacc gctggcgaaa ttatcggtct gcaaatgggg ctgtcatttg cgacatttat
    12541 cgatcctggc gccatctcaa tatgcccgtt ttagcgcgca tcatggatat gctggcatta
    12601 ctgctgttcc tgacatttaa cggtcattta tggttgattt cactgctggt cgataccttt
    12661 cacaccctgc cgattggtag cgaaccgttg aacagcaatg cgtttctggc acccaccaaa
    12721 gcagggagtt tgattttcct taacgggctg atgctggcgt taccgctcat tactctgttg
    12781 ctgaccctga atctggcatt aggtttactg aatcgtatgg ccccgcaatt atccattttt
    12841 gttattggat ttccattaac tctgactgtc ggcatctctt taatggcggc attaatgccg
    12901 ttaattgcgc ctttttgcga acatttattc agtgaaattt ttaatttgct ggctgatatt
    12961 attagtgaat taccattaat ataattccgt aacgtttatc atgctaccct aaggattatc
    13021 cgaaaaataa tatctgcgaa catcttccag gatactcctg tagcgaaata tttgttttaa
    13081 gctcactcac atatcgcaac atttacttta agacaattcc aggcaaatta tacaacactt
    13141 tacgggatag taagtccgcc tgaaaaattg cgagagtggc gcattaggtg acccatgttg
    13201 ttccgtttag tcatgatgaa atattcaggt aaggggaatt atcgttacgc attgagtgag
    13261 ggtatgccat gtcaacgatt attatggatt tatgtagtta cacccgacta ggtttaaccg
    13321 ggtatctgtt gagtagaggg gttaaaaaaa gagaaatcaa cgacattgaa accgttgatg
    13381 accttgccat agcttgtgat tcacagcgtc cttcagtggt gtttattaat gaggactgtt
    13441 tcatccacga tgcttctaac agtcagcgca tcaagcacat cattaatcaa catcccaata
    13501 cgttatttat cgtttttatg gcaattgcca atgttcattt tgatgaatat ctattggtca
    13561 gaaaaaattt attgatcagt tctaaatcga ttaaaccgga atctctcgac gatatccttg
    13621 gcgatattct gaaaaaagag acaacgataa cctcgttttt aaatatgccg acgttatcat
    13681 tgagccgaac cgaatcgagt atgttgcgaa tgtggatggc aggtcaggga accattcaaa
    13741 tctccgacca aatgaatatc aaagccaaga ccgtttcatc gcataaaggt aatattaaac
    13801 gtaagatcaa aacgcataat aaacaggtta tctaccatgt cgtccgactg acggataatg
    13861 tgactaatgg tatttttgtc aacatgcgct aacacattct gactggtggt ttcccaccag
    13921 tcaggctgaa taagattact ctgctttctc aacaaagata ccgtcctgat gccctgcttc
    13981 attaaagaac cagatgccga gcgggtagtc ttccagcgca accaggtaca ttgtgccttc
    14041 actaaactcc tcaactgcca gtaccactcc aggacgacgc ggaccgccat ccgttttgac
    14101 tgttacccga tcattcacct tcatcgtttt cctcctgtgg ctttgtgcca gtgtagaaca
    14161 atttcgtttt tctggcagcg ccaagcgcgc gcgagtgctg attttctcga ctgtctatac
    14221 ttaagagatg ccaggcggac ttaacgactg gcggcaacaa cagagtaacg gttgcgagga
    14281 aagatgatga aaaccgcaaa agtgtacagc gataccgcaa aacgtgaggt cagcgtcgat
    14341 gtcgatgccc tgctggcggc gatcaatgaa attagcgaaa gcgaagttca tcgcagccag
    14401 aacgattctg aacacgttag cgtcgatgga cgtgaatatc atacatggcg tgaattggcg
    14461 gatgccttcg aactggatat tcatgacttc agcgtctctg aagtgaatcg ttgaatgcac
    14521 aataaaaaaa tcccgaccct gagggtgtcg ggatgaaact tgcttaagca agaagcactt
    14581 aaaaaattcg ttacaccagg aaatctgatg tgttcatcac cttatctgca atttttttcg
    14641 ctgacaagaa aatattcgcg ctatgaatga ttaacgctac ttatgaatag aaatgtgacg
    14701 cgcgtcaccc cttggtggct ctggtgtcaa cgggatcgct cccgaaaaaa tcatctttga
    14761 ttatttgtgc tgttttgaga atcatcctgg taaggggagt actgcgcgac catgttttca
    14821 attcaacaac cactactggt ttttagcgat cttgatggca ccctgctgga cagtcatagt
    14881 tatgactggc aaccggcagc cccctggctc agccgtttac acgaagccaa tattcccgtc
    14941 attctctgta gcagtaaaac ttcagcggaa atgctgtact tgcaaaaaat gttggggcta
    15001 caaggcttac cgctgattgc agagaatggc gcagtgatcc agcttgctga acaatggcag
    15061 gatatagacg gttttccacg catcatctca ggtattagcc atggcgaaat ctgccaggtt
    15121 ttaaatacgc tacgcgagaa agaacatttt aaattcacga cttttgatga tgtcgacgat
    15181 gcaaccattg ccgaatggac gggattaagc cgtagctagg cggcgctgac gcagctgcat
    15241 gaggcgtcgg taacgctaat ctggcgcgac agtgacgagc gtatggcaca atttatcgct
    15301 cgtctgaacg aactgggctt gcagtttatg cagggtgcgc gcttctggca cgtactggat
    15361 gcctctgccg gaaaagatca ggctgccaac tggattatcg cgacctatca acaattgtca
    15421 ggcagacgcc caaccacact tggcctgggc gatgggccaa acgatgcgcc tttactggag
    15481 gtaatggatt acgcggtgat tgtgaaaggg ctaaaccgtg aaggggtaca tctgcatgat
    15541 gaggatccgg cccgcgtctg gcgaacgcag cgtgaaggac cggaaggctg gcgtgaaggg
    15601 ctggaccatt ttttctccgc ccgttaagcg ttatcgctcg cgaatacccg attgcgcccg
    15661 gcctgttttg cgaggtaaag ccgacggtcg gccaatgact ggagttgttc aaaatcataa
    15721 tcaccggttt cctcgctgct acttaccccc agcgaggcac tgatgcgtat cgttgtactc
    15781 ttggcgatca gcatctcttt ttcatttaac ttcaggcgaa tacgttctgc gacctccgca
    15841 gcctgcgtca gattcgcgcc tggcagaatc acacaaaact cctcaccacc gacccgcccg
    15901 gcaacgtcct gcgcacgcaa ggaactgcta attaatccgg cagcatgaga aagaacacgg
    15961 tcgcccgcct gatgaccaaa gcggtcatta atcgctttaa aatggtcaag atcgacctga
    16021 atgacagaaa aaggatgttg gtgcgtctga cacaatttag cgagcggacg ggctttttcg
    16081 aacagtgcgc cacggttata taaacgcgtt aaggtgtcgt gccacgcctg ccactgcaac
    16141 gagctttgca gaacatacat gttgctgacc atccggcgaa tcacatacca ggaaaggagt
    16201 aacatggtgg taaagagcgc ccacagcagg gttaatgcaa tgctgatact gccgaaatcg
    16261 ccgcgcacgc cttcgcttag cgtatggacg cgcgccagca caccgtcgaa atggtccaga
    16321 cgttcccagc taacatagcg actgtccata cgaatgcagc cccgcgtgtc atgttccatt
    16381 gcctgcgcca gcaaggccag ttcacgagga tcaaaaatat tccctgttgg atggtcagga
    16441 ttggaagagg tcaaaaatat cagcttactg tcatagagct gatactcacc atcgaggttt
    16501 ttatcgatgg cgtttcttaa aaattgctgc atagtacgca cgggaatact catccccagc
    16561 acgccatacc agtagttatt actgtctacc ggaacactga cggtaacctg cggttcagta
    16621 ttgctggcgt gttccggttg cgaagtgaac cagcgtaccg cgcggtgacg attttctcgt
    16681 tgcgaatggc cgataaacca gggttgggtg acatagccgt aataacgcgt tggtacattg
    16741 cgcataaaca aggtcggctg cgtcgaaacg taaaatccag cacgcgagac atacatcgcc
    16801 tgttcaacca tcgacgagga gttgtgcgcc agtcgcagca agtaaccaac ttccagtgca
    16861 gcggtaattt cattgtcgag gctttcattt tcgcgagaca ggaaattccc ctcgctgact
    16921 aatgcatccg atacgccatt gactggcaga gtgcgccgtc ggttgagttc gatttgccag
    16981 gcgtgctcgt cgcgatgctg ttcgaactcg gttacggcat tacgcagaga ggtgaaatcc
    17041 agtggcgcca cgagagcttc gcgcatgcca ttacgcaaaa agatcagttt gtcgacgtta
    17101 tactgcaaat gcttatcgag cgcgttggct acgttttcca gatgattacg ctggctggag
    17161 atataggcat cttccagcac caccacttca cgccaggtga gcaaggtgga aaaaagcaat
    17221 accacgataa agcagagatt aacgacatga ccgggcccca ggcggcgtgc gagttttttc
    17281 aaccagctct ggttttccat ttttgtctcg tgctgcaccc tgacgactcc caacctgctt
    17341 ttttatgatt ctggcataac ccggcaatag cgt
//
LOCUS       DAETPZ010000079        17241 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.079, whole genome shotgun sequence.
ACCESSION   DAETPZ010000079 DAETPZ010000000
VERSION     DAETPZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.079"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..172
                     /locus_tag="K0W59_003016"
     CDS             <1..172
                     /locus_tag="K0W59_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HBI3023962.1"
                     /translation="CSRSRLGERPKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGA
                     VFGGELTLVQLQ"
     gene            complement(173..364)
                     /locus_tag="K0W59_003017"
     CDS             complement(173..364)
                     /locus_tag="K0W59_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HBI3023963.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(469..705)
                     /locus_tag="K0W59_003018"
     CDS             complement(469..705)
                     /locus_tag="K0W59_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HBI3023964.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(823..2262)
                     /gene="rsmF"
                     /locus_tag="K0W59_003019"
     CDS             complement(823..2262)
                     /gene="rsmF"
                     /locus_tag="K0W59_003019"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HBI3023965.1"
                     /translation="MAQHAVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFGGRVFGASVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPVPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2342..4975)
                     /gene="yebT"
                     /locus_tag="K0W59_003020"
                     /pseudo
     CDS             complement(2342..4975)
                     /gene="yebT"
                     /locus_tag="K0W59_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4944..6228)
                     /gene="yebS"
                     /locus_tag="K0W59_003021"
                     /pseudo
     CDS             complement(4944..6228)
                     /gene="yebS"
                     /locus_tag="K0W59_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
     gene            6358..6855
                     /gene="msrC"
                     /locus_tag="K0W59_003022"
     CDS             6358..6855
                     /gene="msrC"
                     /locus_tag="K0W59_003022"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HBI3023966.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6952..7650
                     /gene="proQ"
                     /locus_tag="K0W59_003023"
     CDS             6952..7650
                     /gene="proQ"
                     /locus_tag="K0W59_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HBI3023967.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7670..9718
                     /gene="prc"
                     /locus_tag="K0W59_003024"
     CDS             7670..9718
                     /gene="prc"
                     /locus_tag="K0W59_003024"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HBI3023968.1"
                     /translation="MSMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLERSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMMAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9910..10791
                     /gene="htpX"
                     /locus_tag="K0W59_003025"
     CDS             9910..10791
                     /gene="htpX"
                     /locus_tag="K0W59_003025"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HBI3023969.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10837..12210)
                     /locus_tag="K0W59_003026"
     CDS             complement(10837..12210)
                     /locus_tag="K0W59_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3023970.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12387..13178
                     /gene="kdgR"
                     /locus_tag="K0W59_003027"
     CDS             12387..13178
                     /gene="kdgR"
                     /locus_tag="K0W59_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HBI3023971.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13321..13560)
                     /locus_tag="K0W59_003028"
     CDS             complement(13321..13560)
                     /locus_tag="K0W59_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023972.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            13719..13862
                     /gene="mgrB"
                     /locus_tag="K0W59_003029"
     CDS             13719..13862
                     /gene="mgrB"
                     /locus_tag="K0W59_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HBI3023973.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13937..14224
                     /locus_tag="K0W59_003030"
     CDS             13937..14224
                     /locus_tag="K0W59_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HBI3023974.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14894..15037
                     /locus_tag="K0W59_003031"
     CDS             14894..15037
                     /locus_tag="K0W59_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HBI3023975.1"
                     /translation="MCGIFSKEVLGKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            15050..15259
                     /gene="cspE"
                     /locus_tag="K0W59_003032"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             15050..15259
                     /gene="cspE"
                     /locus_tag="K0W59_003032"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBI3023976.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15425..16234
                     /gene="rlmA"
                     /locus_tag="K0W59_003033"
     CDS             15425..16234
                     /gene="rlmA"
                     /locus_tag="K0W59_003033"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HBI3023977.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16231..16797)
                     /gene="mntP"
                     /locus_tag="K0W59_003034"
     CDS             complement(16231..16797)
                     /gene="mntP"
                     /locus_tag="K0W59_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HBI3023978.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGSDDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4554 a         4171 c         4347 g         4169 t
ORIGIN      
        1 gtgtagccgc tcgcgattag gtgaacgccc aaaagggcag ggaattacag gtgctgatca
       61 tttctggttt ggtcatacac cgttgcgaca tcgcgtggat attggcaacc tgcattatat
      121 tgataccggt gctgtctttg ggggcgaact gactcttgtg caactgcaat aattaaaaat
      181 caccatactc ctgtgctggt cgccagaaac catctataaa atcctcaatc ggaaaacaac
      241 cgccatggcg gatccgttga tcgctcatag aataaagaca ctgctgttcc gtgttgtaga
      301 catccacaac aatatcttca caaccgccat ccaggtagca aacaaaaagt accagcgcga
      361 acatttcatc cccgaagtgt ggtgccgtac cgttaagttt aggagagatt ttacaacggg
      421 ggaataacca ggacaaataa cccgccatgc cgacgggttc tttttggatc aggcaagacg
      481 cataatccag gcatcagatt gttgatcgta acgggtacgg taaaggacag agtgttcgcc
      541 atttagcagg gcagggatac tggtttcagc atcccaggca tccagttgca tacataaatc
      601 ggggtcagat ttacaaggaa tggttaacgt tcgatcaccc ggcgattcac cgtgtaattc
      661 gccgtcgtcg atttcaaaag cgcctatgcg cacactggtt ttcatcttcg tgctctccat
      721 ttagctgctg attgtggtac gaccagtccg atcgcacaat ttttcagcct agttgaggcc
      781 gccgaatgtg ccagtcaaaa agtgcgcttt ttttgtgcgc tgtcatgcgt tactggtaaa
      841 aagtttcccg tcccgcacca gttcacgcgg gtagctgttt ttcagtcgcg aaccaatccg
      901 tttggctaaa ccaatcggct gatgctggaa agtaaccaac acgtcatccg ccactggcgc
      961 ggcttgcggg taaacatcgc gcccgcgata ccactcctcc gcttcctgcg gtgtcagttc
     1021 aaaagcgttt acattgtcgg gggaggcatg ggcaataacc gcttcatgct gccagcgata
     1081 acctttgtta tgcgtttcgg caagtttaat ccctaagcga gaaaatcgga ctttaccgat
     1141 cagggcttca atgcctaccg ggaacaacca cagttctttg tcgcgttgcc agagtcgcag
     1201 gtttccatcc cagtttaagc caacacttgc agccgcctga cgaatttgtc cggcttcgcg
     1261 atctttcacc gggctgaacg ggaaattgcc cactttgtat tttgggacgg gtaaggcggg
     1321 gatcgcctga gttttacgca gacgagcaac gaagaagcct tcgcagtcgt aaatttgtgg
     1381 gaaaacatgc aaaaaacctt cttcggtcag cgccttgttt gcaccaggga agagatcgcc
     1441 aagtggtaaa aactctactt cgtcggggta agtctctttc agccaccggc aaacggcttc
     1501 gttttcttcc cggtttaagg tacaggtcga gtaaaccagc gtaccgccag gacgtaatgc
     1561 atgaaaggcg ctgtcaatca gttcccgttg ggtagctgcg atttcctgat tgctttcggg
     1621 tgaccagttt tttagcgcat cgggatcttt acgcaccacg ccttcgccgg agcagggagc
     1681 gtccagcaaa atggcatcaa acatttctgg cactgacgca ccaaacacgc ggccaccaaa
     1741 atgtgtgagc gcaacattac tgatgccaca gcggctgata ttggcatgta acacttttac
     1801 ccgactggcg gaaaactcat tggcaaggat tgccccttcg ttattcatcc gcgcggcaat
     1861 ttgcgtcgtt tttgagcctg gcgcagcagc gacatccatc acccgctgtg gtgcattacc
     1921 gtcagcaaac aaggcggcga cgggcaacat tgaactggct tcctgaatat aaaacaggcc
     1981 acttaaatgc tcggtggtac tacccaatgg caatgcatct tcattgtcgc gttcaatcca
     2041 gaaaccttct tcacaccacg gaattggcgt aagcgtccag ccataaggag cggttaattg
     2101 caggaaatca gcaacggaga ttttcagcgt attaacgcga atgctgcggc gcaacgggcg
     2161 ctgacaggcg gcaagaaaat catcaaatga gagcgtcgaa ggcatagctt cgcgcatttg
     2221 tgtcagaaac gcgtccggga aataaacggc gtgttgggcc acgagcatgt accactggca
     2281 aaaaaacagg cgcgcagttt agcataaacg ctccggcgca ggcacgccgg agcagtgggc
     2341 attatttggg aagcgcagtt ccccattcac gccactcttt cggttcactt tcctgcaaca
     2401 ggaagtgttt gccttcctgg gctttcggtg caagtggcgt acccggaggc gtggcgaagg
     2461 cgatgccgcc acggataaat tggttaaagg tgccggtttt cactacgccg cccgtcagac
     2521 caaagtccag actgtaaccc gatgccaacc agaagacgga attgttacgc accaggtgtt
     2581 gatagcgttt actgatgcgc atcgcaatca tcacgcgatc tgacaatgtc cccagcgtca
     2641 ttccggtaac cgtacctact tccagaccac ggaacagcac aggcgtaccg atgcctaacg
     2701 aaccggcttc cggcgcttca acaataatgc ttaaaccatc taagtaacgc gaatcagtaa
     2761 tggtggcctc ttctaattca aagtcgcgac gaggattgcc acggcctggt tcgacgttga
     2821 tatacggctg gaggatagta tctagatgct caacgccagc tgccgagatt tgcggtgtga
     2881 ccactgagaa gcgcgtacca ccgcgagcaa aggtctggac atattccgga tagagcaccg
     2941 cctttgcctg tacttcattg cgtgcggtaa tcagatccag cgtctggatt tgcccgatat
     3001 caataccgag atagcgaatt ggcatcccga ccgccagttt tccggcatcg aaagcgtgaa
     3061 gcgtaatctg cctgccaacc gcacgggccg ctgtttcgga agcatacaga attcgcttgt
     3121 cgcctttacg ctgactggcg ctggcaccac tgaggttatc gaagctaatg gcccccttta
     3181 atgctctgga gagcggggat gcctgtacgg tcaggccact accattcagc tgaactttcg
     3241 ccccgccttc tgcctagaac acgctgttgc tggtcaaaag gttgcgatat tccggtttaa
     3301 tgtgcagatc gatatcgaac gcgttagctc gcggacgcac ggtaataact tcaccaactt
     3361 caaatttacg gtacagcact accgatcctg cctgcacatc cggcagcgtc tctgcactca
     3421 aactcacggt tgtggtgggt aaatcgctaa ggctgttctc cagcgctttt tccagattgg
     3481 catacagtgg atagctggct ttcatctcgc ctttatcgcc cggcagaata cgtatcccgc
     3541 cgttaatcca ttctgaggcg ctggcaccga gaaactcaac gccatccagc cccaccttca
     3601 cgtcgacacg gctgttgacg acaaatttgc tatcgccttt taccagttct cgatgctgag
     3661 gctcgatggc gacggtaaag gtgacgcctt tgctggtgag tttacgatcg ataacctggc
     3721 ctacctgcac gccgtgaaga atgagcggct gacccgcatc aataccgtaa ctttccggtg
     3781 ctgtcagggt cagtgtcaga acgtccggct cttgcagcag ggctttttcg cccggcacaa
     3841 caacgaactc tttgcgtggc tcgccatcgc cgggcaccaa ctcaaaggtt tttccggtca
     3901 gcagggcgct gagattagca tcgctaaggg ataatttcgg gttgcgtaat tctatgcggg
     3961 tattttcacg aagcagggta acgacgctgg gatcaacggt catttcccca gtgactttac
     4021 caccaggatt taaatccagt ttagtcagct gtccgacttc cagcccctga tacattaacg
     4081 gcgtcgagtc ggcggttaat cctgctccac tcggcagttc cagttttatt attactccac
     4141 gctggctgtg ggccagatct tcatacagac caaaggtatc ttccgcctcg gcaggtttcg
     4201 actcttcagg tgaatcgaag gcaatcgcac cgttaaccag tgccgccaga ctttccagtt
     4261 tcaccttcgc gccactgata ctgacgttgg catcaacgcc ggaaacgttc cagaaacggc
     4321 tacctttttt caccagatcg gtaaaacgcc gctcgatcag gacatcaatc accacgcctt
     4381 gcttgttggg attgatggca tagtcgtaga cttttcccac cgggatcttg cggaaataga
     4441 ccaatgaacc gctgttcagc gaaccgagat cgggggcttg caggtggatc atcagatcgc
     4501 cattgtccag ccgatatttc ggttgggtat cgagcgcgac aaagtgatcc tgctctttac
     4561 ctttacccgg catcatgccg atatagttcc caccgacgag ggcgtccagc ccggagacac
     4621 ctgccaacga tgcttttggc gtcatcagcc agaactgagt ctcttcgcgc agcgcatctt
     4681 tcatatcgga cttgatgctg accttgactt caatcttacg aagatcgtcg ctgaggctga
     4741 tatcctgcac tgttccgact tcaacgccct gataacgaac aggcgtacgg cccggaacaa
     4801 taccatccgc cgacataaag tcgatggtga cggtattacc ccggtcctga taactgtccc
     4861 aaatcagcca accggcaatc attagcgcga tgaaaggcag cagccagaaa ggtgagatac
     4921 ggcgtttatt tttaatctgc gcttcagtcg tcgaagcggg cgtttcctga ctcatgtgca
     4981 tcccaaagta agcggctgtc cagccattcc acagcaagaa tagtcaaaat tacggcagcg
     5041 ccaaaataaa acgcagccgg tcccatagta aaagcgagga tctgatcgcg attaatcagc
     5101 gacatggtta aagatatgac aaacaggtcg agcatcgacc agcgtccaat ccaggtcacc
     5161 atccgcagta acagaatgcg tgtgcgtaaa ccttgctggc atttaaaatg aatgctcaac
     5221 agtaaagtga acatgacgat cactttagta aacggtacca gaatactggc gataaacacg
     5281 attcccgcaa ccgcaatgtt gctacttgcc agcgacataa ttccggaaag aatcgtatct
     5341 tcctgccggc caccattcag ataaatgata gaaataggca ataggttggc tggtaacaac
     5401 aaaacgattg acgctaacag cgccgcccag catttttgca aactatgacg acgacgcagg
     5461 cgtagcggga tatggcaacg cgggcagaga ccacgctgat caggatagcc ggtaaaatgg
     5521 cacccaagac agacacgaag tttctcgtcc ctacgcgtag cggggcgctg cggataatat
     5581 cgctcccaca gctcctcaac attaagatgt gacaacgtca ccgtcgttaa aatcaccaac
     5641 gccacaaaag agaacaagcc gacacccgcc tggatatggg cataatcctg taccttatat
     5701 agaagcaacg ccaatgccga ccaggtagat atccagcatt acccactctt taagtcgctc
     5761 aagcatcagc agtaccggac gtaaattcat tcccagtcgg ttaccaaacc ataaatatgc
     5821 tatggaggac accagaatga ggggggcacc aataacgcag aaaaagacca ttgatcccgt
     5881 tatcgcatcg ccctgtttgg tcatttgcca gatgccttgc atgacgttgg cgtcaatacg
     5941 aatgcctaac agccagatat gcaacagcgg ttcgccccag gcaaacggca taagcaacag
     6001 catggtgaag gccattgccg ccaggcgcgt tagcgacaag tcgcgcccgt cgcgaatttt
     6061 tgcctgacag cgcggacaat aggcactttg atgagaattt atctcgggca ggctaaacag
     6121 catgtcacat tgcgggcaac gttggtaatc accacgcggc agttcctcgc cgattgccct
     6181 cactgttatc ttttttgtcg gcgtaatttg tggtgtgtta agagccatga cgtgccaaat
     6241 gtaataagaa ttaacattaa tcttaaccca tgatgggaat gatcttgagc attaacgcat
     6301 ttaatgctta ttttaatagg ctggcagttc caggtaagac aaccaagtga ttaaataatg
     6361 aacaaaacag aattttacgc ggatttaaat cgcgacttta acgcgctgat ggcgggagaa
     6421 accagttttc tggcaacgct tgcgaacacc agtgcgttgt tatacgagcg tctcactgac
     6481 gttaactggg caggttttta tttgcttgag gacgatacac tggtactcgg accatttcag
     6541 ggcaaaattg cctgtgtccg gatacccgtc gggcgcgggg tgtgcggcac agcggttgcc
     6601 cgcaatcaag tgcagcgtat cgaggatgtt cacgcgtttg acgggcatat tgcctgtgat
     6661 gcggcgagta attctgaaat tgttctgccg ctggtggtga aaaatcagat tattggtgtt
     6721 ctcgacatcg atagtaccgt cttcgctcgc tttacagacg aggacgagca aggcttacgt
     6781 cagcttgtgg cacagcttga aaaagtgctt gcaacgacgg attacaaaaa attctttgcg
     6841 agcgtcgcag gataatcaac ggataacgta gcatttactg gtagcgtcat tataatgacg
     6901 cctgttcatg cctgcgcttg ttggctacgt ccgttgtaat caggaaattt catggaaaat
     6961 caacctaagt tgaatagcag taaagaagta atcgcgtttc tcgccgaacg ttttccccac
     7021 tgtttcagtg cggaaggtga agcgcgtccg ctgaaaattg gtatttttca ggatttggtc
     7081 gatcgtgttg ccggggaaat gaacctgagc aaaacgcaat tacgatccgc tttacgtctc
     7141 tacacttcga gctggcgtta tctttacggt gttaaacccg gcgcaacgcg tgtcgatctt
     7201 gacggcaacc catgcggtga gctggacgag caacatgtag agcatgctcg caagcagctt
     7261 gaagaagcga aagcgcgtgt tcaggcacag cgtgctgaac agcaagcgaa aaaacgcgaa
     7321 gctgccgcag ctgctggtga gaaagaagac gcaccgcgcc gcgaacgcaa gccacgtccg
     7381 accacgccac gccgcaaaga aggcgctgaa cgtaaacctc gtgcgcaaaa gccggtagag
     7441 aaagcgccaa aaacagtaaa agcacctcgc gaagaacagc acaccccggt ttctgacatt
     7501 tcagctctga ctgtcggaca agccctgaag gtgaaagcgg gtcaaaacgc gatggatgcc
     7561 accgtattag aaatcaccaa agacggcgtc cgcgtccagc tgaattcggg tatgtctttg
     7621 attgtgcgcg cagaacacct ggtgttctga aacggaggcc gggccaggca tgagcatgtt
     7681 ttttaggctt accgcgttag ctggcctgct tgcaatagca ggccagacct tcgctgtaga
     7741 agatatcacg cgtgctgatc aaattccggt attaaaggaa gagacgcagc atgcgacggt
     7801 aagtgagcgc gtaacgtcgc gcttcacccg ttctcattat cgccagttcg acctcgatca
     7861 ggcattttcg gccaaaatct ttgaccgcta cctgaatctg ctcgattaca gccacaatgt
     7921 gctgctggca agcgatgttg aacagttcgc gaaaaagaaa accgagttag gcgatgaact
     7981 gcgttcaggc aaactcgacg ttttctacga tctctacaat ctggcgcaaa agcgccgttt
     8041 tgaacgttac cagtacgctt tgtcggtact ggaaaagccg atggatttca ccggcaacga
     8101 cacttataac cttgagcgca gcaaagcgcc ctggccgaaa aacgaggctg agttgaacgc
     8161 gcagtgggac agtaaagtca aattcgacga gttaagcctg aagctgacag gaaaaacgga
     8221 taaagaaatt cgtgaaaccc tgacacgccg ctacaaattt gccattcgtc gtctggcgca
     8281 aaccaacagc gaagatgttt tctcgctggc aatgatggcg tttgcgcgtg aaatcgatcc
     8341 gcataccaac tatctttccc cgcgtaatac cgaacagttc aacactgaaa tgagtttgtc
     8401 actggaaggt attggcgcag tgctgcaaat ggatgatgac tacaccgtta tcaattcgat
     8461 ggtggcaggt ggtccggcag cgaagagtaa agctatcagc gttggtgaca aaattgtcgg
     8521 tgttggtcaa acaggcaagc cgatggttga cgtgattggc tggcgtcttg atgatgtggt
     8581 tgccttaatt aaagggccga agggcagtaa agttcgtctg gaaattttac ctgctggtaa
     8641 agggaccaag acccgtactg taacgttgac ccgtgaacgt attcgtctcg aagaccgcgc
     8701 ggttaaaatg tcggtgaaga ccgtcggtaa agagaaagtc ggcgtgctgg atattccggg
     8761 cttctatgtg ggtttgacag acgatgtcaa agttcaactg cagaaactgg aaaaacagaa
     8821 tgtcagcagt gtgatcatcg acctgcgtag caatggcggt ggggcgttga ccgaagcggt
     8881 atcgctctca ggtctgttta ttcctgcggg tcccattgtt caggtccgcg ataacaacgg
     8941 caaggttcgt gaagacagcg ataccgacgg gcaggtgttc tataaaggcc cactggtggt
     9001 actggttgac cgtttcagtg cttcggcttc agaaatcttt gccgcggcaa tgcaggatta
     9061 cggtcgtgcg ctggttgtgg gtgaaccgac gtttggtaaa ggcaccgttc agcaataccg
     9121 ttcattgaac cgtatttacg atcagatgtt acgtcctgaa tggccagcgc tgggttctgt
     9181 gcagtacacg atccagaaat tctatcgcgt taacggcggc agtacgcaac gtaaaggcgt
     9241 aacaccagac atcatcatgc cgacgggtaa tgaagaaacg gaaacgggtg agaaattcga
     9301 agataacgcg ctgccgtggg atagcatcga tgccgcgact tatgtgaaat caggagattt
     9361 aactgccttt gaaccggagc tgctgaagga acataatgcg cgtatcgcga aagatcctga
     9421 gttccagaac atcatgaagg atatcgcgcg cttcaacgct atgaaggaca agcgcaatat
     9481 cgtttctctg aactacgctg tgcgtgagaa agagaataat gaagatgatg cgacgcgtct
     9541 ggcgcgtttg aacgaacgct ttaaacgcga aggtaaaccg gagttgaaga agctggatga
     9601 tctaccgaaa gattaccagg agccggatcc ttatctggat gagacggtga atatcgcact
     9661 cgatctggcg aagcttgaaa aagccagacc cgcggaacaa cccgctcccg tcaagtaata
     9721 tcaatcaggc acaagaaatt gtgcctgatt ttttaacagc ggcaagatgc cgtaaatcag
     9781 atgctacaaa atgtaaagtt gtgtctttct ggtgacttac gcactatcca gacttgaaaa
     9841 tagtcgcgta acccatacga tgtgggtatc gcatattgcg ttttgttaaa ctgaggtaaa
     9901 aagaaaatta tgatgcgaat cgcgctcttc ctgctaacga acctggccgt aatggtcgtt
     9961 ttcgggctgg tactgagcct gacagggata cagtcgagca gcgttcaggg gctgatgatc
    10021 atggccttgc tgttcggttt tggtggttcc ttcgtttcgc ttctgatgtc caaatggatg
    10081 gcattacgat ctgttggcgg ggaagtgatc gagcaaccgc gtaacgaaag ggaacgttgg
    10141 ctggtcaata ctgtagcaac ccaggctcgt caggcgggga tcgctatgcc gcaagtggct
    10201 atctaccatg cgccggacat caacgctttt gcaaccggtg cgcgccgtga tgcctctctg
    10261 gttgctgtca gcaccggttt gctgcagaac atgagcccgg atgaagccga ggcggtaatt
    10321 gctcacgaaa tcagccacat cgccaatggt gatatggtca ccatgacgct gattcagggc
    10381 gtggtgaaca ccttcgttat ctttatttcc cgtattctgg cgcagcttgc cgcgggtttt
    10441 atgggcggaa atcgtgatga aggtgaagag agcaacggca acccgctgat ctactttgcg
    10501 gttgcaacgg ttctggaact ggtgtttggt attctggcga gcattatcac catgtggttc
    10561 tcgcgtcatc gtgaattcca tgctgatgcc ggttcggcaa aactggttgg tcgcgagaaa
    10621 atgattgccg cgctgcagcg cctgaaaacc agctatgaac cgcaagaagc aaccagcatg
    10681 atggctttct gcattaacgg taagtcgaaa tcgctcagtg agttgttcat gactcacccg
    10741 ccgctggata aacgtattga agctctgcgt acgggtgaat acctgaagta atatgaagta
    10801 aatactaaaa agcgcgtcct gatcgacgcg ctttttttat gccctggctc gtggctgagt
    10861 gatacgtaaa ccactgacac aggcagcaat cactgccaga atagccgcag ccatcagcga
    10921 gacgtgcgta ccattatcac caaactgatt tagcatcagc gccaccagcg ccgcgccact
    10981 actctgaccc agtagacgag ccgttcctaa catgccactg gccccaccgc tacgttcgcg
    11041 cggagcggag gtaataatgg tgtggttatt gggtgactgg aataagccaa atccagcgcc
    11101 acataagatc atcggccaga taatattgat atccgcaggt gacgcgggca gcagaaccag
    11161 ggaaaaaagc cccgcagcca tgatgaacaa ccctaaagca cccagcaatc ctgcatgtac
    11221 gcgttcaatc aaatagcctg ccagcggagc catcaccatc gttgctaacg gccacggtgt
    11281 cagaagtaaa cctgtttcga cttcactacg cccgagcacg gtttgcaggt aaaagggcag
    11341 ggaaaccatt gccagcattt gtgcgcagaa agagcaaaca gatgtgcaaa tagaaagtga
    11401 aaacagcggg atacgcagta aatccaccgg tagcagcggt acgggaagag aaagctggcg
    11461 gcgaataaag aaaataccaa caacaaccat taccaccagt tccgcagcaa ttaacgtcag
    11521 cgattgcccc tgagcgaaac cactcaacgc agtgataagc aggccgaagg ttaacgcgtt
    11581 catcacggcg ctgggcaggt cgaaacgggg tttactggcg cgagaaccat tgggtggcag
    11641 aaaacgcatc gccagaagca gggcgataat acctaacggt acgttgatta aaaataacca
    11701 tttccaggat gcgatggaga ggattgctgc agcaattgtc ggcccggcag cagaagagac
    11761 ggcaacaata aacgagttta tgcccatccc tctccccaga aaacgttgtg gatagatcag
    11821 gcggataagt gcggtattaa cgctcatcaa cgctgcaccg ccgaaacctt gtatgacacg
    11881 cgcaagggtg agcatttgca gcgaatcaga aagggcgcag aacagtgaag acaacagaaa
    11941 aacgaccaga ccgcatttat aaatacgtcg atagccaaac atatcgccca gaaacgaaaa
    12001 cgagagcagg gagatgacaa tggcgatttg ataggcgttc actacccaga tggaactggc
    12061 tggtgtggca tgaaggtccg tggcgattgt tggcagggcg acgttggcga ttgcgccgtc
    12121 aaggacagcc atcgaaatac caatcacaat ggttaatatc gcaccgtatc gctggggcaa
    12181 tggcaggccg tcggcctgaa cttttggcat aggaatttta tatctttggt gaataatcaa
    12241 caggggtatg attctagcac tgatattcac ggtggatgtc gcagaattgt aactaaccgg
    12301 caaaagatgg attgcgacca ccctggtgcg aaattataat aaaaaacagt tctgattttt
    12361 ataaaacact cgcaatgagg tgataaatgg ctaacgcaga tctggataaa cagcctgatt
    12421 ctgtatcttc cgtgctaaaa gtttttggca ttttgcaggc gctgggtgaa gagcgcgaaa
    12481 tagggataac cgagctgtcg cagcgcgtca tgatgtcaaa aagcaccgtt tatcgctttt
    12541 tacagaccat gaaaacctta ggttatgtgg cgcaggaagg ggagtcggag aaatattccc
    12601 tgaccctgaa attgtttgaa ctgggcgctc gcgcgttaca aaacgtcgat ttaattcgta
    12661 gcgcagatat ccagatgcgt gagatctccc gcctgaccaa agaaactatc cacctcggcg
    12721 cactggacga agacagtatt gtttacattc acaaaattga ctctatgtac aatttgcgca
    12781 tgtattcacg gattgggcgt cgtaatccgc tgtacagcac cgcgattggt aaggtactgc
    12841 tggcatggcg cgatcgcgat gaagtgaagc aaattcttga gggcgtggag tataaacgca
    12901 gtaccgagcg gaccatcacc agtacagaag cgttattacc cgttctggac caggtgcgcg
    12961 agcaggggta tggcgaagat aacgaagagc aggaagaagg gctgcgatgc attgcggtac
    13021 cggtatttga tcgctttggc gtggtcattg ccggtttgag catctccttc ccgacgttgc
    13081 gtttctctga agagcgttta caggaatatg tcgcaatgtt gcataccgca gcgcgcaaaa
    13141 tttctgccca aatgggttat cacgactatc cgttctgatg agagtaagaa cctgtcggaa
    13201 tatcaaacag acaggttctt tatttagcat gagaaaaata aagttgaagg tgcgttatat
    13261 taaacgcgct tgctataaga atattttact catgagtgag aatctggtta tttattaccc
    13321 ttaaccatta tcgaccacga tattgctttt acgtaacagt gggcaatctg ttatccccaa
    13381 gaaaccactt ttagtgtgca agtattgtac cgtgctggtg cctctggcag tcagataggt
    13441 acattgcaaa cctaatcctg cggcattctc tttgcttcca atcaaaacgc catatccgct
    13501 gagtaataat cctatccata ccagtgctat cagcataact gtgcgaatga tgaatcgcat
    13561 tacaatctct tctcttttta tgttcgctta atcgtagcgg caatatgcgc tgaagcaagc
    13621 tactcattcc gaaaaagcac gaatatcgac atagttaggc gctgtttaac taacgcatgc
    13681 tagtttaatg gcataaggta ggtgaagcgg agattggagt gaaaaagttt cgatgggtcg
    13741 ttctggttgt cgtggtgttg gcttgcttgc tgctttgggc gcaggtattc aacatgatgt
    13801 gcgatcagga tgtacaattt ttcagcggaa tttgtgccat taaccagttt atcccgtggt
    13861 gatatcattt tttcagcact agattttctt cctctccagc agtggtagaa tagcgcctct
    13921 actcagaggt ggtgaaatga acgaagttgt aaattcaggc gtgatgaaca ttgcgtcttt
    13981 ggttgtatcg gtggtggttc ttcttatcgg gctcatcttg tggtttttta tcaatcgtgc
    14041 cagttcacgg actaacgaac agattgaact gcttgaggcg ttgctggatc agcaaaaacg
    14101 tcaaaatgca ctgttacgtc gtttgtgcga agcaaacgaa ccagagaaag cagataaaaa
    14161 gaccattgag agtcaaaaat cggttgatga cgaagatatt attcgcctgg tcgccgaacg
    14221 ataatccttc ctcaatcttg agtaaccccg tcgttgaagg gttactcaat tcatcctgat
    14281 tatacgcact gtcatggtgc aactgcttca aattaaaact ctggtctgac ttcaaataat
    14341 tatcatatta tttatttgcg taacgggata tcagaaaaag atattttcct cattaattta
    14401 ttaggatgtt tacatcagat ttgtgattaa gcgtggtatt atttattagc gaaacgtttc
    14461 tctcttgatt tttttgctca ctcatcaatt tttcttattt taaatttaca atccctttgg
    14521 ggattgactt ctctttaggg taattgatag ccgttaactg actgttttat gagaaaaagt
    14581 gatataactt tttattcatt gcatagcaga aaatgtgata ttgcacgcac tatgtaataa
    14641 cttctccctc tggcctggaa caactgaact tattgaacta tgttagaaac tacgccagtt
    14701 taagtatctg cctgaactgg caaggttaag cacaatgata tatcggcgcg tattccgttg
    14761 cataagtgtg caaaaaaagt ggaagacgta tcgagatttg tgcgtctgat cgagacatgt
    14821 ttaaaaatgg cttgccataa ttaacgttgt atgtgataaa agatttcggg ttaaacgagg
    14881 tacagttctg tttatgtgtg gcattttcag taaagaagtc ctgggtaaac acgttgacgt
    14941 tgaataccgc ttctctgccg agccttatat tggtgcctca tgcagtaatg tgtcagtttt
    15001 atctatgtta tgcctgcggg cgaagaaaac aatctaagga atttttcaaa tggcaaagat
    15061 taaaggtcag gttaagtggt tcaacgagtc taaaggtttt ggcttcatta ctccggctga
    15121 tggcagcaaa gatgtgttcg tacacttctc cgctatccag ggtaatggct tcaaaactct
    15181 ggctgaaggt cagaacgttg agttcgaaat tcaggacggc cagaaaggtc cggcagctgt
    15241 taacgtaaca gctatctgat cgaatccact gatctgaagt gtgaatacgc ttcaatctcg
    15301 ctataaagcc tcgtcgaatg cgaggctttt tactatgctt tatcttcgct cctggcgttc
    15361 ggatatttgc cagccacgtg attcgcgtta cacttgcggc ctttagtatc ctgccggagt
    15421 tgtcatgtcg ttttcctgtc cactttgcca tcagcctctt tcgcgtgaaa aaaacagcta
    15481 tatctgtccc caacgacatc agtttgatat ggcgaaagaa gggtatgtca atctgctgcc
    15541 cgttcagcat aaacggtctc gtgatccggg cgacagcgcg gaaatgatgc aagcacgccg
    15601 cgcattctta gatgccggac attatcagcc gctgcgtgat gcaattgtcg cccaactgcg
    15661 ggaacggctt gatgaaaagg ccacggcggt gctggatatt ggctgtggtg aagggtatta
    15721 cacacacgca tttgccgatg cgttgcccga aatcaccacg tttggcctgg atgtctcgaa
    15781 ggtagcgata aaagcggcgg cgaaacgcta tccgcaggtc actctttgtg tcgcttccag
    15841 ccaccgtttg ccgttttccg ataccagtat ggacgccata atacgtattt acgcgccgtg
    15901 taaagcagaa gaattagcac gagtagtgaa gcccggcggc tgggtcatta ctgccacgcc
    15961 gggaccgcga catttgatgg agctgaaggg gctgatttac aatgaagtac atcttcatgc
    16021 acctcatgca gaacaactgg aaggttttac attacagcag agtgcggagt tgtgttatcc
    16081 gatgcgtctt cgcggtgatg aagccgtcgc attattgcag atgacgccgt ttgcctggcg
    16141 tgcgaagcca gaagtctggc aaacactggc agcaaaagaa gtgttcgact gccagacgga
    16201 ctttaatatt cacctctggc agcgttctta ttaaccgtgg aagtgcgtcc agaggatctg
    16261 gacgccgatg ccgatcagca ccagcccgcc gagaatttcc gcttttttcc caataattga
    16321 gccgataaag cgaccaacca tcatccctaa tgtcgacata atcaaggttg cacaaccaat
    16381 ggccaatgcg gtcgcgataa tgttgacctg aaggaaagca agaccaacac ccacagccat
    16441 ggcatccagg ctggtggcaa tcgcggtggt taccagtagc cagaaaccgt gccgacggcg
    16501 cggctcttca tcttcatcat ctgagccacg aaaaccctca ataatcattc gcccgccgag
    16561 gaatatcagc agcacaaacg caatccagtg attccattca aggacaaacc ggctggctaa
    16621 catgcccatt ccccagccga tcagcggcgt cagggtttcg acggcaccaa aaataaggcc
    16681 ggttcgcaat gcttcagaaa atttcggttt atggaggggg gcacctttac cgattgatgc
    16741 agcaaatgca tccatcgaca taccaaacgc aagaagaaca gtagcagtga tattcatgac
    16801 aacgtcctga ccggggtatc cataaaacac atcatcgccc ccagcaaaca tgcatacatg
    16861 aacaacaagc cgcgatgggt tttcgcgttc atgcaggcat agcgattgat gtgcatatgg
    16921 tctcgcctga ctgaaagtct tcagtccgta cgtgccacgt tttgcaacga gtatgttgac
    16981 acgtacattt ccggaatctg gaaatcggct actccccaaa tgagggcgca accttaacat
    17041 attttgagaa tataaaacaa ctgttggaat atattatttc attgctttat tgataacgat
    17101 tttcatttat gtttatatat caatgaaacg ttaaaataaa taacaaataa gggaagagat
    17161 aatatagcct aagctatgtc tgattattaa aaatacacta aacatagctt tggctaaatt
    17221 cataagttat tgagttgata c
//
LOCUS       DAETPZ010000080        17183 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.080, whole genome shotgun sequence.
ACCESSION   DAETPZ010000080 DAETPZ010000000
VERSION     DAETPZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.080"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(464..1660)
                     /gene="hemY"
                     /locus_tag="K0W59_003035"
     CDS             complement(464..1660)
                     /gene="hemY"
                     /locus_tag="K0W59_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HBI3023979.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1663..2862)
                     /gene="hemX"
                     /locus_tag="K0W59_003036"
     CDS             complement(1663..2862)
                     /gene="hemX"
                     /locus_tag="K0W59_003036"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBI3023980.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQGDTP
                     AAAPQGE"
     gene            complement(2884..3624)
                     /gene="hemD"
                     /locus_tag="K0W59_003037"
     CDS             complement(2884..3624)
                     /gene="hemD"
                     /locus_tag="K0W59_003037"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HBI3023981.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFTIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3621..4562)
                     /gene="hemC"
                     /locus_tag="K0W59_003038"
     CDS             complement(3621..4562)
                     /gene="hemC"
                     /locus_tag="K0W59_003038"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBI3023982.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            4949..7495
                     /gene="cyaA"
                     /locus_tag="K0W59_003039"
     CDS             4949..7495
                     /gene="cyaA"
                     /locus_tag="K0W59_003039"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HBI3023983.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENSFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7535..7855)
                     /gene="cyaY"
                     /locus_tag="K0W59_003040"
     CDS             complement(7535..7855)
                     /gene="cyaY"
                     /locus_tag="K0W59_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HBI3023984.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7903..9216)
                     /locus_tag="K0W59_003041"
     CDS             complement(7903..9216)
                     /locus_tag="K0W59_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HBI3023985.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTP
                     DLVIDRSFNSGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            complement(9213..10691)
                     /locus_tag="K0W59_003042"
                     /pseudo
     CDS             complement(9213..10691)
                     /locus_tag="K0W59_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            10880..11083
                     /locus_tag="K0W59_003043"
     CDS             10880..11083
                     /locus_tag="K0W59_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBI3023986.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11120..11944
                     /gene="dapF"
                     /locus_tag="K0W59_003044"
     CDS             11120..11944
                     /gene="dapF"
                     /locus_tag="K0W59_003044"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBI3023987.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            11941..12648
                     /locus_tag="K0W59_003045"
     CDS             11941..12648
                     /locus_tag="K0W59_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HBI3023988.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12645..13541
                     /gene="xerC"
                     /locus_tag="K0W59_003046"
     CDS             12645..13541
                     /gene="xerC"
                     /locus_tag="K0W59_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBI3023989.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13541..14257
                     /gene="yigB"
                     /locus_tag="K0W59_003047"
     CDS             13541..14257
                     /gene="yigB"
                     /locus_tag="K0W59_003047"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HBI3023990.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14341..16503
                     /gene="uvrD"
                     /locus_tag="K0W59_003048"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14341..16503
                     /gene="uvrD"
                     /locus_tag="K0W59_003048"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HBI3023991.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACSELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(16650..17042)
                     /locus_tag="K0W59_003049"
     CDS             complement(16650..17042)
                     /locus_tag="K0W59_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HBI3023992.1"
                     /translation="MRPGGVFYVAGDKVGIVRLDAFKASKDIQFAVQSGPMLMENGVI
                     NPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVEQLLYLGGT
                     ISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         3979 a         4448 c         4557 g         4199 t
ORIGIN      
        1 ttgatgctgt ttttagtttt aacggcaatt aatatatgtg ttattaattg aatgaatttt
       61 atcattcata ataagtatgt gtaggatcaa gctcaggtta aatattcact caggaagtta
      121 ttactcagga agcaaagagg attacagaat tatctcataa caagtgttaa gggatgttat
      181 ttcccgattc tctgtggcat aataaacgag tagatgctca ttccatctct tatgttcgcc
      241 ttagtgcctc ataaactccg gaatgacgca gagccgttta cggtgcttat cgtccactga
      301 cagatgtcgc ttatgcctca tcagacacca tggacacaac gttgagtgaa gcacccactt
      361 gttgtcatac agacctgttt taacgcctgc tccgtaataa gagcaggcgt ttttttatgc
      421 atcagaaagg tcccggaggt gctttcctcc gggtgagaag gaactactgt ggcgggttat
      481 tctgcaacgt taacatcaaa ccgtcgcgac gcatcgctgc agcttcttcc ggcttgtgca
      541 gtctgtccag cgcgtcggca agccatgcgt aatcgtaggc gtccggacgt tgtttcagcg
      601 ccgcgcggaa ggcgagcgat gcttcctgcc attcaccgtg cttcatcagt gactgaccca
      661 atgtgctcca caacagcggg cgatcaccga cgtttttgat ttgctggcgt agcacttttt
      721 ccagctgttc cgggttattg gttttcagac gcgggatcgg cagcagtagg cgatcgtcat
      781 actggcgttt caggccgtcg atgataattt gctgggcagt atcatgatcg tcacattcaa
      841 taagatgttc cgccattgcc acctgcaacg ctacctgatg acgcgttttc cggctttggt
      901 ttttccacca gttacgcaaa ccttcactac cgttatcggc acgcgcctga tccatcaggc
      961 caatccatgc ctgttgttcc agcattgcac gatgttcttc atcaccaaca tgggctttcg
     1021 ccattgatgg gatgatatcc agcagagaac tccatgcccc tgtgcggata tacgcctgtt
     1081 ccgccagacg taatacttca ggatggcgtg gcgtaacttc cagcagctta tccacgccgt
     1141 ggcgtgcagc atggttttca ttacgggcca gttgcagacg tacacgggtg atttctaccg
     1201 gaatggtgtc attgccggcc agctccgctg cgcgttccag atgttggttg gcgcgtgctt
     1261 catcaccacg ttgttgcgcg gcttcggcag ccagtagata gttcaccacc ggttgttcag
     1321 cgtgatcggc atttttcgcc atcagctttt caacttgctg ataatcacct tccgccagtt
     1381 tcagcagcgc ctgttcggtc tgcttacgcg cacggcgacg cttacgtccg acaaaccatc
     1441 cacgggtgtg cgcgccagtg cggaagatcc gccgcagtag ccactcaatg gcaaacagca
     1501 ctaccatcgc cagaatcaat atgatcgcca ggcccgtgac gctggtttcg atattgtagt
     1561 tgtcggtctg gatcagcaca taaccctgat gaccggcaat catcgggcca accactatcc
     1621 ccgcaatcag caaaacaaag agtaataaca cttttagcat ggttattctc cttgcggcgc
     1681 ggctgccgga gtatcacctt gcggtgcagg tgcaggtgca ggtgcaggtg caggtttagc
     1741 ttccgttgtc cccgctgccg gttgtgccag caggttacgc acgcgagtct gcatcagttt
     1801 ttccagcatc gcctggcttt gcagggtttc cggaagatcc atcgagatat tttgctggct
     1861 taactggtcc acctcgtcga ggaacgcttt ggtggtggca tcatcagtat cgtagtaagc
     1921 acgtacccag gtggagacgt tctccagcgc ctggcgataa gtctcttcct gatgacgcgg
     1981 tacagcttgt gctgcgacca gcaggcgaga gcgaatattt tcgcgcagat agatatcctg
     2041 atttggcgct aacagcggta cggcggtgtc atcacgacgg cgaatcgtaa tgaagttgtc
     2101 cataaagttc tgccagcttt tttgcagatt gatacgccat tcgctgatgg aactggaaag
     2161 ctcttcaccg tctgaatcca tcggcgaacc atcgctgtca ttatcggcca gacgcaggtt
     2221 atctacctga tttgaaagct gattaagctt aaggatgatg ccgtcataat ccacctgcga
     2281 tactgcagaa aggctggcga tatcatcggt aattgcccga cgaacggtaa tcagactcgg
     2341 gtcattcata tccgccaggc tggcgtctgc acttttcagc aacgctgcag aggtcgtgac
     2401 gtcctgatcg ctccacagct tccgtccggc gagtttcacc agaaaatcgg cctgagccag
     2461 cagccaggtt ttagcatcgc tgccggaaat ggtggcgatc ttttgttgga cttcatccaa
     2521 ctgttttgcc agcgtttctt gctgacgatt cgcctgctta agctgcgcag cttgttgctt
     2581 aataatgcct tccagctcgg ctttttggct ctcctgggct ttttgcaatg ccgtcagttg
     2641 gttagccagg gcatcgctgg tggcggtctg attgacggcc tgttgtttac cccagccata
     2701 caaaccgaca cccgccgcca gagcaatagc gatagccacc gcgctgagaa tcaatgcagt
     2761 attgttctta ctcttttttt ctgttgcgac aggttgtgac gtggtgtcca cggcctccct
     2821 ggtctcttca accacggcgg aggttttttc ttgttccgtc attatggctt cctgttatga
     2881 gagttattgt aatgcccgta aaagcgcatc gttgtcagcg ttatcggcga ccttaatgtc
     2941 ttgccagccc agttcccggg cgagtttcgc caaacgctca ctgacgacca atagtcgaca
     3001 gtgtagtaac cagtgctcac gataccattg tgggatcagc gaccagagtt gctgcaacat
     3061 ttcaccgctg gtaacaacga ccatcgtcac ctcgcgggat tgccagcgca tcgcttcttc
     3121 tgcaccatcg taatggattg cgcatcgttg ataacattca caaaaagtga cctcagcacc
     3181 gcgcaccgtc agggtatccc caattagctc acgaccgcca ttgccacgta atatcagcgc
     3241 acgtttgccc gcaatatttt gtaattcagg taattgtagc aagacttcgc tgatttcccg
     3301 atcctgtggg tagagaatct tctgtccgct tacggtatgt agcgccagtg cggtggtgcg
     3361 tccaatggtg aaataagtag gtagttgggg ccatttacga tcttgctgat gcagctgtga
     3421 ttgggcaaaa gcaaccgcgt gttgcgagag ggcaaacaac agatcgctct cccccagcgc
     3481 tgccagttga tcagcaagtt gcggtaattg tcgacccgga gaaaactcaa tcagcggaaa
     3541 atgccaggcc acctgcccca gtgtgcgcag acggctcact aactcttctc cagcgggaga
     3601 cgggcgggtg accaggatac tcatgccggg gcctctccgt tatagacttc agcgaggatc
     3661 tcgcgcgcgc cgttattcag tagctcttct gccagcgaaa tccccatttg ttcggcatct
     3721 tgcggcgcac cgcggcgttc accgcgaata atctgcgaac cgtccggcgc gccgaccagc
     3781 gcacatagcc agatttcgcc atcaataagc tcggcgtagc taccaattgg cacctgacat
     3841 ccgccttcga gacgggtatt catggcgcgt tctgcggtaa cgcgcagtgc agtttcgtgg
     3901 tgattcagcg cggcaagcag ctcgcgagtg cgtgtatcat caaggcggca ttcaataccg
     3961 actgcacctt gtcctaccgc cggaagagaa atctcgggtg gtaacgcggc gcgaatgcgg
     4021 gactccagac ctaaacgttt tagtccggct acggcaagaa tgatggcatc gtattcgccg
     4081 ttatctagtt tgctcaggcg agtgccgacg ttgccgcgca gggagcggat aatcagatcc
     4141 gggcggcgtt cagccagttg gcactggcga cgtaaactgg acgtcccgac gatactgcct
     4201 gctggtaacg catccagact gtcatagtta ttggacacaa aggcatcgcg aggatcttca
     4261 cgctcgcaaa tagtgaccag ccccagacct tgcgggaatt caaccggcac atctttcatt
     4321 gaatgtacgg cgatatcagc gcgattctcc aggagcgcga cttccagctc ttttacaaat
     4381 aagccttttc cgcctacttt cgccagcggc gtatcaagaa tcacatcgcc gcgcgtcacc
     4441 atcggtacca gttcaacgac caggcccgga tggctcgcca tcaacttgtc tttgacatag
     4501 tgtgcctgcc agagtgcaag tgggctttgg cgtgtggcaa ttcttaaaac attgtctaac
     4561 atgcttgtta ccgtcattat catccgtggt ccatcctaac atccttgcca gagtgatgtc
     4621 agtgttgtgg tgaaacgtag acgcctgcgc aaaccgtaaa atgaggtctg gcagtggatc
     4681 ctgacaggcg tttcacgccg tcgtaataag gaatttacag agaataaacg gtgctacact
     4741 tgtatgtagc gcatctttct ttacggtcaa tcagcaaggt gttaaattga tcacgtttta
     4801 gaccattttt tcgtcgtgaa actaaaaaaa ccaggcgcga aaagtggtaa cggttacctt
     4861 tgacatacga aatatcccga atgccgcgtg ttaccgttga tgttggcgga atcacagtca
     4921 tgacgggtag caaatcaggc gatacgtctt gtacctctat attgagactc tgaaacagag
     4981 actggatgcc ataaatcaat tgcgtgtgga tcgcgcgctt gctgctatgg ggcctgcatt
     5041 ccaacaggtc tacagtctac tgccgacatt gttgcactat caccatccgc taatgccggg
     5101 ttaccttgat ggtaacgttc ccaaaggcat ttgcctttac acgcctgatg aaactcaacg
     5161 ccactacctg aacgagcttg aactgtatcg tggaatgtca gtacaggatc caccgaaagg
     5221 tgagcttcca attactggtg tatacaccat gggcagcacc tcgtccgtag ggcaaagttg
     5281 ttcctctgac ctggatatct gggtctgtca tcaatcctgg ctcgatagcg aagagcgcca
     5341 gttactacaa cgtaaatgca gcctgctgga aagctgggcc gcctcgctgg gtgtggaagt
     5401 cagcttcttc ctgattgatg aaaaccgctt ccgtcataat gaaagcggca gcctgggggg
     5461 cgaagattgt ggctccaccc agcatatact gctgcttgac gaattttatc gtactgccgt
     5521 gcgtctcgcc ggtaagcgta ttctgtggaa tatggtgccg tgcgacgaag aagagcatta
     5581 cgacgactat gtgatgacgc tttacgcgca gggcgtgctg acgccaaatg aatggctgga
     5641 tctcggtggc ttaagctcgc tttctgctga agagtacttt ggtgccagcc tttggcagct
     5701 ctacaagagt atcgattccc catacaaagc ggtgctgaaa acactgctgc tggaagccta
     5761 ttcctgggaa tacccgaacc cacgtctgct ggcgaaagat atcaaacagc gtttgcacga
     5821 cggcgagatt gtatcgtttg gcctcgatcc atactgcatg atgctggagc gtgttactga
     5881 atacctgacg gcgattgaag atttcacccg tctggattta gtacgtcgct gcttctattt
     5941 aaaagtgtgc gaaaaactca gccgtgaacg cgcctgtgta ggctggcgtc gcgcagtgtt
     6001 gagccagtta gtgagcgagt ggggttggga cgaagctcgt ctggcaatgc tcgataaccg
     6061 tgctaactgg aagattgatc aggtgcgtga ggcgcacaac gagctgctcg acgcgatgat
     6121 gcagagctac cgtaatctga tccgctttgc gcgtcgcaat aaccttagcg tctccgccag
     6181 tccgcaggat atcggcgtac tgacgcgtaa actgtatgcc gcgtttgaag cattgccagg
     6241 taaagtgacg ctggtaaacc cgcagatttc acccgatctc tcggaaccga atctgacctt
     6301 tatttatgtg ccgccgggcc gggctaaccg ttcaggttgg tatctgtata accgcgcgcc
     6361 aaatattgag tcgatcatca gccatcagcc gctggaatat aaccgttacc tgaataaact
     6421 ggtggcgtgg gcatggttta acggcctgct gacctcgcgc acccgtttgt atattaaagg
     6481 taacggcatt gtcgatttgc ctaagttgca ggagatggtc gccgacgtgt cgcaccattt
     6541 cccgctgcgc ttacctgcac cgacaccgaa ggcgctctac agcccgtgtg agattcgcca
     6601 tctggcgatt atcgttaacc tggaatatga ccctacagct gcgttccgca atcaggtggt
     6661 gcattttgat ttccgtaagc tggatgtctt cagctttggc gagaatcaaa attgcctggt
     6721 aggtagcgtt gacctgctgt accgcaactc gtggaacgaa gtgcgtacgc tgcacttcaa
     6781 cggcgagcaa tcgatgatcg aagccctgaa aactattctc ggcaaaatgc atcaggacgc
     6841 cgcaccgcca gatagcgtgg aagtcttctg ttatagccag catctgcgcg gcttaatccg
     6901 tactcgcgtg cagcaactgg tttctgagtg tattgaactg cgcctttcca gcacccgcca
     6961 ggaaaccggg cgtttcaagg cgctgcgcgt ttctggtcaa acctggggct tgttcttcga
     7021 acgcctgaat gtatcggtac agaagctgga gaacgccatc gagttttatg gcgctatttc
     7081 gcataacaaa ctgcacggct tgtcagtgca ggttgaaacc aatcacgtca aattaccggc
     7141 ggtggtggac ggctttgcca gcgaagggat catccagttc tttttcgaag aaacgcaaga
     7201 cgagaatagc tttaatatct acattctcga cgaaagcaac cgggttgagg tgtatcacca
     7261 ctgcgaaggc agcaaagagg agctggtacg tgacgtcagt cgcttctact cgtcatcgca
     7321 cgaccgcttc acctacggct caagcttcat caacttcaac ctgccgcagt tctatcagat
     7381 tgtgaaggtt gatggtcgcg aacaggtgat tccgttccga acaaaatcga ttggcaacct
     7441 gccgcccgcc aatcaggatc acgatacgcc gctattacag cagtattttt cgtgatgagc
     7501 gtgccggaaa gcgaggctta tccggcatgc aatcttagcg gaaactgact gtttcacccg
     7561 cctgttgcgt cgccgcctgt tccagcaaat cccagaaggt ttcgccgctg cgatcgcaaa
     7621 tccattcatc gcctttcagg tcaaaatggt agccgccctg tttggttgcc agccacacct
     7681 ggtgcagcgg ctcctggcgg ttaataatga ttttgctgcc gttttcaaag gtaatggtca
     7741 gtacgccgcc gttgatctcg cagtcgatat cgctgtcgcc atccctgtcg tccagacgct
     7801 cttcaatggt cagccacagt tgatcagcca ggcgatgaaa ttcactgtcg ttcattgttg
     7861 taccctgttt ttaagtgatg gcggcagtat agcggcatgg ggtcagggct tcaaagtttg
     7921 cacctctgct gctgcgttcc ggcatgattc atccgtcacc ggaataatga tgtctctgtt
     7981 ttgaacttca cgtacgttct cctctctgac acgagttttc gccgggcagg gggagggcgt
     8041 aatccttacc tctgtatcgg tccatgtata aacattgcga tcgtcataag cgaagcgatc
     8101 gtttttagtt agcggaccgt taactggaat aatttgcaag ctctttgacc gtaattggcg
     8161 gataaagtaa gcatgctgtt tgccgctatt aaatgagcga tcaatgacca agtctggcgt
     8221 atccagcggg attatctcaa gctggtcttc accaaatgtc gataattttg ttacatacag
     8281 ccgatcttta tattgcgcaa tattgccagt aatcagatgg aattgttcag gttcaatacc
     8341 tggtaggtct atccacttgc tccactgacg gtcgggcgtg agctgtttac gccagcgaac
     8401 gcgactttcc cctacttcaa aagagacgtc atcatctacg ggtatcgctt tgcctgataa
     8461 ttgaccaaag ggataacgat gcagaccctt attgtcgcga aaaaccagca gtgaatgagg
     8521 tatccagcga attatctcaa atgagtctgc attcgccgta aggtgaacgc catttaaata
     8581 aatattctgc ccactgcgga taagcagatc attggaatat gaatttctgt ttacactcat
     8641 aagacgtgga ttaatacccc accatctttt ttctaataca gtaacatcac tgctgctccc
     8701 ctgacgtcga ccggacaagt agagattttt actatccacc agcgtggtag agttaccttc
     8761 gatagctttt agtgtcgcca gatcaacacg tgacgcgccg ctattacttt cggtgcgctg
     8821 tccgtcaaaa tagaggctat atttatcgac cgcgaagcgg cccatagcct gaaagctggc
     8881 aatatcgaca gtgggtgttc ccggtggatt gctaacgatt ttgccacgcc agagaataac
     8941 gcggccgtct gtcacccagt ggtattcctc attttctatg cttatttcat cggaaggtat
     9001 ataacccgtt ttcccagaga tatttttcac aaggcgcaaa ccggccctgc tcatctccgg
     9061 aaactgggca atgattttct tatcccccca ataaccttta ccgtaagaca taacaccgtg
     9121 atcattgtaa tagtcgaacc ctatggcctg tacagcaggc gtcagaaatg tgtgtcccaa
     9181 caggatgagt gcaactgtga ttatttgacc cttcattttg tcacctcatt gcgcggggca
     9241 ggaataaatt cgccgtcatc agtaattttt ccgtccagat atcccgtttt gtaatttacg
     9301 ccatagacat atcccgaatg ttgttccttc cagattaagc gaacttgttt cggatcagcg
     9361 gtgcggtatc gccagagctg cgcgccatca aagctgtaaa tgtagcggtt atcgcgggca
     9421 taccatctgc gcaaaaaggg atggtcatta agatgggctt cgcgttcatc cggagaacgg
     9481 ttatccagta agatcagcgg tccgtcagat tcaaacacgt gtaaaccgga atggtatggc
     9541 tacccttcgg cacgcgtaag taaatagccg tggcgtttcc cggcgttgaa acgtttgttg
     9601 ataataagat tgggatcatc aagtttcacg atctccagct tacggtcacc agactctgtt
     9661 tcgatgatgg tataaagcga gtcctgatac tgggcgacag catcactgat gggttgaaac
     9721 tgctccggat cgagtccggg taattcttcc tgtttacaag caggtccttt acgccagaac
     9781 actttctttt ccagcagaga aaaatccaga cagtgctttg ttaaatcttc atcccacgcc
     9841 agattttcct tgtcgaggac tttacgctgc agcccgtttt tatcacgcca ggtcaagagt
     9901 gagccgggca tccagcgcac gacggaaaag gtgtccgggt cggcatcaag ctgttcgccg
     9961 ttgagcagga ttttggtccg tgtacgagcc agggtatccc aggggccaaa tggtacggca
    10021 atgcaggggt taaacgtggt ggaaaacttt cctcgctgat cccaggactt ctgcgccagc
    10081 acacggaaac tctcggggtc agcgaggcga tgcccattga tatacaagaa attagcgtca
    10141 cgtaatatca gccccagtaa gtcaggatcc cagggaggcc ggaattccac ctgatgcagc
    10201 gttttgatat ccaagctatt tccaccacca ttgtcatcgg tgcgcttgcc ttcaaaatag
    10261 agactgtgtt tatctgcggc aaaatcaccc catgccttga acgaggcgac atcgacggga
    10321 ggtgtgccgg gagggttttg cacaacttca cccgcgtaga ggatattgcg accatcgcta
    10381 tgccatgaat aatgaggata ctaggcctgt gcgccctcat agaggtagtc ggttatcaaa
    10441 tcagtcagag gcggttcttt atcccattcg tctagtgttg tcgggtctat taataaattg
    10501 ggtaaccgac gcaaactctt gtaattcacg tcaggtagag aaagatttgt ttctttataa
    10561 gggcgcaggt acaccacgcc ttttccctgt ttctggtaaa tattatgttc aggtgcggaa
    10621 agccgacatc cccatgccga cgataaaaac aatgaacaag ccaacaaaca aaccacattg
    10681 cgatagtgca taaagccatc ctggcgcgag gtgttgatca cgaaactgcc agcagaacat
    10741 aaatcctcac gagtaagcgt tatactcgca gcatttcctc acttttcaga cttcataaag
    10801 agtcgctaaa cgcttgcttt tacgccttct cctgcgatga tagaaagcag aaagcgatga
    10861 actttacagg caatccataa tgaaaaacgt gtttaaggca ctcactgtat tacttactct
    10921 cttcagcctg acgggctgcg gtctgaaagg tccgctctat ttcccgcctg cagataaaaa
    10981 cgcaccgccg ccgaccaaac cggtagagac gcaaacgcaa tccacggtgc cggataaaaa
    11041 cgatcgcgcc actggcgatg gtccatccca ggtgaattac taaaagtcag tttctgtacc
    11101 cgcgtgattg gagtaaatga tgcagttctc gaaaatgcat ggccttggca acgattttat
    11161 ggtcgtcgac gcggtaacgc agaatgtctt tttttcaccg gagctgattc gtcgcctggc
    11221 ggatcggcac ctgggggtag ggtttgacca gctgctggtg gttgagccgc cgtatgatcc
    11281 tgaactggat tttcactatc gcattttcaa tgctgatggc agtgaagtgg cgcagtgcgg
    11341 taacggtgcg cgctgctttg cccgttttgt gcgtctgaaa ggactgacca ataagcgtga
    11401 tatccgcgtc agcaccgcca acgggcggat ggttctgacc gtcaccgatg atgatctggt
    11461 ccgcgtaaat atgggcgaac ccaacttcga accttccgcc gtgccgtttc gcgctaacaa
    11521 agcggaaaag acctatatta tgcgcgccgc cgagcagaca atcttatgcg gcgtggtgtc
    11581 gatgggaaat ccgcattgcg tgattcaggt cgatgatgtc gataccgcgg cggtagaaac
    11641 gcttggtcct gttctggaaa gtcacgagcg ttttccggag cgcgccaata tcggttttat
    11701 gcaagtggtt aagcgcgagc atattcgttt acgcgtttat gagcgtgggg caggagaaac
    11761 ccaggcctgc ggcagcggcg cgtgtgcggc ggtggcggta gggattcagc aaggtttgct
    11821 ggacgaagaa gtacgcgtgg aactccccgg cggtcgtctt gatatcgcct ggaaaggtcc
    11881 gggtcacccg ttatatatga ctggcccggc ggtacatgtc tacgacggat ttattcatct
    11941 atgaagcaac caggggaaga actgcaggaa acactcacgg agcttgatga ccgggcggtt
    12001 gtcgattatc tgattaaaaa tcctgagttt tttatccgca atgcgcgcgc agtagaagcg
    12061 atacgtgtgc cgcatccggt acgcggcacc gtttcgttgg tcgagtggca catggcccgc
    12121 gcacgtaatc acattcatgt tctggaagag aacatggcgt tgttgatgga gcaggctatc
    12181 gccaacgaag ggctgtttta tcgcctactc tacctgcagc gcagtctcac ggccgccagc
    12241 agtctcgacg atatgctgat gcgctttcac cgctgggcgc gcgatctcgg cctggctggt
    12301 gcgagtctgc gcctgtttcc agatcgctgg cgcttaggtg cgccgtcgaa ccacactcat
    12361 ctggcattaa gccgtcagtc tttcgaaccg ctgcgtattc agcgtttggg gcaggaacag
    12421 cactatcttg ggccgcttaa cggaccggag ctgctggtgg tgctaccgga agcgaaagcg
    12481 gtgggatcgg tggcgatgtc gatgctggga agcgatgctg atttgggtgt tgtgctgttt
    12541 accagtcgcg atgccagtca ctatcaacaa gggcagggaa cgcagttact tcatgaaatt
    12601 gcgctgatgt tgccggagct tctggagcgt tggattgaac gcgtatgacc gatttacaca
    12661 ccgatgtaga acgctaccta cgttatctga gcgtggagcg tcagcttagc ccgataaccc
    12721 tacttaacta ccagcgtcag cttgaggcga tcatcaattt tgccagcgaa aacggcctgc
    12781 aaagctggca acaatgcgat gcagcgatgg tacgcaattt tgctgtacgc agtcgccgta
    12841 aagggctggg agcagcaagt ctggcgttac ggctttctgc gctacgtagc ttttttgact
    12901 ggctggtcag ccagaacgaa ctcaaagcta acccggcgaa aggagtttcg gcaccgaaag
    12961 cgccgcgtca tctgccgaaa aatatcgacg tcgacgatat gaatcggctg ctggatattg
    13021 atatcaacga tcccctcgct gtacgcgacc gtgcaatgct ggaagtgatg tacggcgcgg
    13081 gtctgcgtct ttccgagctg gtggggctgg atatcaaaca cctcgacctg gagtccggcg
    13141 aagtgtgggt gatggggaaa ggcagcaaag agcgccgcct gccgattggt cgcaacgctg
    13201 tggcgtggat tgagcactgg cttgatttgc gcgacctgtt tggtagcgaa gacgacgcgc
    13261 tttttctgtc gaaactgggc aagcgtatct ccgcgcgtaa tgtgcagaaa cgctttgccg
    13321 aatggggcat aaaacaaggg ctgaataatc acgttcaccc acataaatta cgtcactcgt
    13381 tcgctacgca tatgctggag tcgagcggcg atcttcgtgg tgtgcaggag ctactgggtc
    13441 atgccaacct ctccaccacg caaatctata ctcatcttga ttttcaacac cttgcctcgg
    13501 tgtacgatgc ggcgcatcca cgcgccaaac gggggaaata atgcgttttt accggccttt
    13561 ggggcgcatc tcggcgctca cctttgacct ggatgatacc ctttacgata accgtccggt
    13621 gattttgcgc accgagcgag aggcgcttac ctttgtgcaa aattatcatc cggcgctgcg
    13681 cagcttccag aatgaagatc tgcaacgcct gcgccaggcg gtacgggaag cggaacccga
    13741 gatttatcac gacgtgacgc gttggcgttt tcgttcgatt gaacaagcga tgctcgacgc
    13801 cgggctgagt gccgaagaag ccagtgcagg cgcacacgca gcaatgatta actttgccaa
    13861 atggcgcagc cgaatcgacg tcccgcagca aactcacgac accttaaaac agctggcgaa
    13921 gaaatggccg ctggtggcga tcaccaacgg taacgcccag ccggaactgt ttggtttggg
    13981 ggattatttt gagtttgtgc tgcgcgctgg cccgcacggg cgctcaaaac cgttcagcga
    14041 tatgtacttt ttggctgcgg aaaaactcaa cgtgccgatc ggcgagatct tacatgttgg
    14101 ggacgatctc accactgacg tgggtggggc aattcgcagc ggaatgcagg cttgttggat
    14161 cagaccggaa aatggcgatc tgatgcaaac ctgggacagc cgtttactgc cacatctgga
    14221 aatttcccgg ttggcatctc tgacctcgct gatataatca gcaaatctgt atatataccc
    14281 agctttttgg cggagggcgt tgcgcttctc cgcccaacct atttttacgc ggcggtgcca
    14341 atggacgttt cttacctgct cgacagcctt aatgacaaac aacgcgaagc ggtggccgcg
    14401 ccacgcagca accttctggt gctggcgggc gcgggcagtg gtaagacgcg cgtactggtg
    14461 catcgtatcg cctggctgat gagcgtggaa aactgctcgc catactcgat tatggctgtg
    14521 acgtttacca acaaagcggc ggcggagatg cgtcatcgta tcggacaact gatgggcacc
    14581 agccagggcg gcatgtgggt aggcaccttc cacgggctgg cgcaccgcct gctgcgtgcg
    14641 caccatatgg acgccaatct gccgcaggat ttccagatcc tcgatagcga agaccagcta
    14701 cgcctgctta agcgtctgat caaagccatg aacctcgacg agaagcagtg gccgccgcgc
    14761 caggcaatgt ggtacatcaa cagccagaaa gatgaaggcc tgcgtccgca tcatattcaa
    14821 agctacggta atccggtgga gcagacctgg cagaaggtgt atcaggcgta tcaggaagcg
    14881 tgcgatcgcg cgggcctggt ggacttcgcc gagctgctgc tgcgcgctca cgaactgtgg
    14941 cttaacaagc cgcatatcct acaacactac cgcgaacgtt ttaccaatat cctggtggac
    15001 gaattccagg ataccaacaa cattcagtac gcgtggatcc gcctgctggc gggcgacacc
    15061 ggcaaagtga tgatcgtcgg tgatgacgac cagtcaatct acggctggcg cggggcgcag
    15121 gtggagaata ttcagcgttt ccttaatgat ttccccggtg ccgaaattat tcgtctggag
    15181 caaaactacc gctctaccag caatattctg agcgccgcta acgccctgat tgaaaacaat
    15241 aacgggcgtc tgggtaaaaa actgtggacc gatggcgcgg acggtgagcc tatttccctc
    15301 tattgcgctt ttaacgaact cgatgaagcg cgttttgtgg ttaaccgcat caaaacctgg
    15361 caggacaacg gcggggcgct tgccgagtgc gccattctct accgcagtaa cgcccagtcg
    15421 cgtgtgctgg aagaagcact gttgcaggcg agtatgccgt accgtattta cggcgggatg
    15481 cgcttcttcg aacgccagga aatcaaagat gcgctctcgt atctgcgcct gattgccaac
    15541 cgtaacgacg acgcggcctt tgagcgtgtg gtgaatacgc caacgcgggg tattggtgac
    15601 cggacgctgg acgtggtacg tcagacatcg cgcgatcgcc agttaacact ctggcaggca
    15661 tgtagtgaac tgttgcagga aaaagccctc gccggacgtg ctgccagcgc cttacagcgg
    15721 tttatggaac tgatcgacgc cttagcgcag gaaactgccg atatgccgct gcatgtacag
    15781 actgaccggg taattaaaga ctccggcctg cgtaccatgt acgagcagga gaagggcgaa
    15841 aaaggtcaga cgcgtatcga aaacttagag gaactggtga cggcaacgcg gcagttcagc
    15901 tacaacgaag aagacgaaga tttaatgccg ctgcaggcgt tcctatccca tgcggcgctg
    15961 gaagcaggcg aagggcaggc ggatacctgg caggatgcgg tacaactgat gaccatgcac
    16021 tcggcgaaag gtctggagtt cccgcaggtg tttatcgtcg gtatggaaga gggcatgttc
    16081 ccaagccaga tgtcgctgga tgaaggcggg cgtctggaag aagaacgccg tctggcctac
    16141 gttggcgtaa cccgcgcgat gcagaaactg acgctgacct acgcggaaac tcgccgtctg
    16201 tatggcaaag aggtttacca tcgcccgtcg cgctttattg gcgagttgcc ggaagagtgt
    16261 gtggaagagg tgcgcctgcg cgccacggta agccgcccgg tcagccatca gcggatgggt
    16321 acgccgatgg tcgagaacga cagcggctac aagctcggcc agcgtgtacg ccacgctaag
    16381 tttggtgaag gcaccatcgt caatatggaa ggcagcggtg aacatagccg tttgcaggtg
    16441 gcattccagg gccagggaat caaatggctg gtggcagctt acgcccggct ggagtcggtg
    16501 taacgttgcc ggatgcggtg ctgcgcacct tatttggcct aaaaaatcat tcagattcaa
    16561 taaattgcaa cgtcatgtag gcaggataag gcgtttacgc cgcatccggc atcagcagca
    16621 tatttagtat ctgacacaaa gctatcattc taacctttcc gctcgacgga aatcatggtg
    16681 acaaaaggat agcgttgcca cgggattgcc ccgcctttca tatacatatg tgaaatcgtg
    16741 ccaccaagat aaagcagctg ctcaacgttc agtttcgctt tggcataaca ggcaaaatca
    16801 taaaaatttg tcgcctgctg gctcaacaaa aacacggcat tcccatgttt attaatccca
    16861 acaccgttac gaattttacg tgaggcgacg ttgggatgaa tacgcggatt aattacaccg
    16921 ttttccatca acattggtcc tgactgcacc gcaaactgaa tatctttact ggctttaaaa
    16981 gcatccagac gaacgatgcc gactttatct cccgcgacat aaaacacgcc gccaggacgg
    17041 ataaagaaat tcccttcacc tgaagcgaga tttaacgcta ccttctgctg taatgccgat
    17101 cagttaagga tcagttgacc gatccagtgg ctgtgtaaga atccggaaac gctcacttgt
    17161 ttccggattt ttttatgcac att
//
LOCUS       DAETPZ010000081        17124 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.081, whole genome shotgun sequence.
ACCESSION   DAETPZ010000081 DAETPZ010000000
VERSION     DAETPZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.081"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(467..1600)
                     /locus_tag="K0W59_003050"
     CDS             complement(467..1600)
                     /locus_tag="K0W59_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023993.1"
                     /translation="MKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVG
                     PDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKP
                     KTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRS
                     VALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISV
                     RNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAK
                     DRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKNLFDIRV
                     LHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            complement(1770..2108)
                     /locus_tag="K0W59_003051"
     CDS             complement(1770..2108)
                     /locus_tag="K0W59_003051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3023994.1"
                     /translation="MSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYN
                     IDPLCECFKY"
     gene            complement(2698..3639)
                     /gene="fabY"
                     /locus_tag="K0W59_003052"
                     /gene_synonym="yiiD"
     CDS             complement(2698..3639)
                     /gene="fabY"
                     /locus_tag="K0W59_003052"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HBI3023995.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3684..4121)
                     /gene="dtd"
                     /locus_tag="K0W59_003053"
     CDS             complement(3684..4121)
                     /gene="dtd"
                     /locus_tag="K0W59_003053"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBI3023996.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(4118..4990)
                     /locus_tag="K0W59_003054"
     CDS             complement(4118..4990)
                     /locus_tag="K0W59_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HBI3023997.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4984..5583)
                     /gene="yihX"
                     /locus_tag="K0W59_003055"
     CDS             complement(4984..5583)
                     /gene="yihX"
                     /locus_tag="K0W59_003055"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HBI3023998.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5682..6467)
                     /locus_tag="K0W59_003056"
     CDS             complement(5682..6467)
                     /locus_tag="K0W59_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBI3023999.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(6501..7397)
                     /gene="yihV"
                     /locus_tag="K0W59_003057"
     CDS             complement(6501..7397)
                     /gene="yihV"
                     /locus_tag="K0W59_003057"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HBI3024000.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            7565..8461
                     /gene="yihU"
                     /locus_tag="K0W59_003058"
     CDS             7565..8461
                     /gene="yihU"
                     /locus_tag="K0W59_003058"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HBI3024001.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPVQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            8485..9363
                     /gene="yihT"
                     /locus_tag="K0W59_003059"
     CDS             8485..9363
                     /gene="yihT"
                     /locus_tag="K0W59_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HBI3024002.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNGLCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAVIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQPGDIVDEMMAKRR"
     gene            9380..10621
                     /gene="yihS"
                     /locus_tag="K0W59_003060"
     CDS             9380..10621
                     /gene="yihS"
                     /locus_tag="K0W59_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HBI3024003.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLFDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWDPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            10759..11661
                     /locus_tag="K0W59_003061"
     CDS             10759..11661
                     /locus_tag="K0W59_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HBI3024004.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            11860..13896
                     /gene="yihQ"
                     /locus_tag="K0W59_003062"
                     /pseudo
     CDS             11860..13896
                     /gene="yihQ"
                     /locus_tag="K0W59_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
     gene            13942..15327
                     /locus_tag="K0W59_003063"
     CDS             13942..15327
                     /locus_tag="K0W59_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3024005.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQKLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTLYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            15370..16773
                     /locus_tag="K0W59_003064"
     CDS             15370..16773
                     /locus_tag="K0W59_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3024006.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            16841..>17124
                     /gene="ompL"
                     /locus_tag="K0W59_003065"
     CDS             16841..>17124
                     /gene="ompL"
                     /locus_tag="K0W59_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HBI3024007.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGL"
BASE COUNT         4269 a         4338 c         4116 g         4401 t
ORIGIN      
        1 gggatattta tttgcataat ctgaatggtg tagccgaaaa acgtcagcat cctaacagca
       61 ccaggattta aggtgaaatt aatctttcat cacagaccgg tcagtactgc caccaaccgg
      121 tctttacttg ctcatcgtta taattttgtt ggtaacagtt cgggaaacaa ggaattcctt
      181 ccggaacacc cccggcatca tccgatagca ttgacgtcaa tcatcagcgc gacgatgagt
      241 ttttttaatc gcatcatagg gtggcgacgt tcagcacgat tacgggtcat agcaaaactc
      301 ctgagcaggc ggccactatg ccgctttcac tggagttctg aatactggta ctttctgtat
      361 ggcttcgcca tgcccctcgc ggctaagtac ctgcacacat ctctctttgt atgtcatttt
      421 tgcaatgcat tgtccgaatt ctgataatcc taatgagttt tactcgtcag actcccatcc
      481 ctacggtata cgagttatat tcatgaatga aatcgacatt tttaaaaaac gaatcttgta
      541 aaacatcagt gttgatatta ccttgcaatc caacattgaa tttgttagag acatgaagaa
      601 ctcgaatatc aaaaaggttt tttaacatca ttaattcttc gggtgttaat gaatcgcaac
      661 taaaatatcc ctgttgatca aaaagatttt ctatttctcc actatcggcg aggcgttttt
      721 caaggagtgt ttgcaacatc gagttgccaa ggacaatgga tatcgtgcga tctttcgcag
      781 atttacaccc catcacaacg gcaacattcg tatccccctc aagagtttca tttatcgccg
      841 tcgctaaaat ggtggtcaaa cacgcaaacg gaactgtatt tctttcgtga tttaatttat
      901 ctaccagttc attccattta tcaaaagccg ggctatattt gttgcgcaca gaaatatccg
      961 actggttaaa caattcagcc atgcgatttt ttatcatctc gatatccggc gaaccagaaa
     1021 gactttgcgc ataatacggt cctgatgatg ttgcatacat taatgccaag cccacacctg
     1081 tcgccatgat gggcgcagcc acggttttgg caagggcgac gctgcgatgg ctatgcgctt
     1141 cacaggctaa aggaacatat tccactgact gcggtaattc cttacatgcc tccctcatca
     1201 ttgcggtatg ctttacaaac aacggttttt cactgcccaa aaaaactttc tgcttaattg
     1261 cactaagaaa acgcaaatca aaaataattg tttttggttt attatttttt ggcacatttc
     1321 ctattatccc gctaagaacc ttccttgctg catggagatt tttttgcaca gcacataaca
     1381 caattgatga agccttctcc actgcattat tgattttaat tttaaaatcc gcatgatata
     1441 cgttgacggg caatgcaagt atatctggac ctacatcttt tgctgagtat ttattggtga
     1501 tcgacggacg tgaacccacg agaaaactta gctgcaaatt atgctctata ttatcagctg
     1561 tttttgtggt gtggttaaac atagagatgc cacctttcat tctttcacac atatcctcaa
     1621 ctttttcact tcgttcttgt ggcaatttgg ctttaacatt aatttgatga tttttacccg
     1681 tgcattcctt aactacaagg ggaatgttat ctaaagtgcc atacaggcta ataacgtcac
     1741 caggattttt atcatgtaag taagtttgat taatacttga agcattcaca aagagggtca
     1801 atattataac tgtttataac ttcacctaca tcaagtgtgt agtgcttact ctggagactg
     1861 ccatccttat ttaattgtat atcaatcaat atatccccgg actttgtcac catatctttg
     1921 ccataaacca tttgcatagc catttcgagg agatttttat ctaactgatt gcgggcgttg
     1981 agtaagcgat taccaaataa tttatatact gttcccgtca ttgccagcgc tgcgctttct
     2041 ggtactttgc gtttccacag tgaggtttct ctatttttaa acaggtcata ttcattatta
     2101 ctcgacaaac catgcttcag ttcgttaaca caatcgcaca gttgctcctc accttccatt
     2161 ttaaggcaaa gtgaaaattg atttaatggc gtttacttcg gtgatttatt actcattagt
     2221 tgtttttcta aattaacaac agaataactt tcttgtttaa tgagattaat attggcaacc
     2281 attatagcct ccttcccacg gtttaagagc aaacatacaa cataatgatt cataacatac
     2341 agagcagtaa atgaatgata ctgacaatgt atgccagccc attatctttg cataatgttt
     2401 gtgagctata gattttaaaa atatttatga aaaaagtatg gcaccagagg gggttttgac
     2461 tatcaaaaat cgctcaaacc tgaactaaga tgatgagtta agcattatct gcaataattg
     2521 ccactcagct ctacaaataa tatttaaatg ctcctgaaca gtgatgtagc aagacacgta
     2581 tggtgccaac gaagaataaa aatttataaa gttcttattc aatataaccg gaatagtcag
     2641 ggactgatgt aaatgaccgg aatgaatgag agaggcagca ccgcccctct ccataagcta
     2701 ctcttcttcg ttcccgccct cttcatacgg gccaaatggc ttcgcgggca gaacgatata
     2761 cgtgccttca aacactgcac ccggcgtctc gtcgccaaag atttcgacct gcatctgcac
     2821 ccgtgctttt cgtccgcgcg ccagacggtc gagatcgccg cttaaggcac caaggtcggc
     2881 taccgcatgg ggtttaccgc taatcggttt gctgtagcgg atatgcgcat ccgccagaat
     2941 aatcgttccg ccgaggtggc gttcacgcag catcagccag ataagccccc aaccggtgag
     3001 cgtcgccagc gagaataaac tcccggcaaa cagcgtatgg tgcggattct gattgccggt
     3061 ttccggcatg gtggtgataa atttttgccc ggtatattgt tgaatacgca cgcccatttt
     3121 ttcactaagc gggatatgtt cgtaccacgc ctgttgcagc tgcgcgcacc agtcgccgcg
     3181 atgcaaaata tcatccagag tggcgacggg cttaatcatc aaaaaatggc gaatcggcgt
     3241 ggtggttggc gttgtgattt ctccctgatt aacaaacccc agcttggcga aaaactccac
     3301 cgcgtcttca cgggcgctac aggtcacgcg cttaacgcct tcctgacgcg ccaccgactc
     3361 cagggtcatc gccatcagcg ttcctaaccc tttgtcctgc acgtccgggt gaacggccat
     3421 aaagcgaatg gacgcttcat tgtcggcatt aatatacaat cggcctaccg ccaccagatt
     3481 accctgctcg tcgacgacca tctggtgatg cgccatcgca tcccacgcgt cgcgttccga
     3541 accttttggt tgatgcaggg gcttacgcaa catttcccag cgaaactgat agtaacgctc
     3601 taactcttct tctgtttgtg gaacctgaag gtgatacata gcgatactct ctcttgtttc
     3661 ccgtgacaac cctggaagct ggctcatacc tgcaaccaga atgtcacggg gccatcattg
     3721 accagcgata cctgcatatc ggcagcgaag cgtcctgttt gcgtgttcat ctcttgctgg
     3781 cggcagcgtt cgacgaaata gtcatataac gcctctgcgc gatccggtga tgcacctttg
     3841 gagaaacttg ggcgcatccc ccgttcggta tctgcggcga gggtaaactg ggaaaccacc
     3901 agtacactgc cgcccgcctg ttgcacgttg agattcatct tgccttcggc atcgctaaag
     3961 atgcggtagc cgagcacacg ctcgcacaga cggtttgctt tctgttcgtc gtcatccttt
     4021 tcgacaccca ataacaccaa aagtcccgcg ccaatttcgc ccgtcacttc tccctccacg
     4081 gtgacgctgg cacgggttac gcgttgaatt aatgcaatca tggttcgtcg tcttcttctt
     4141 gttcagctgc ttgttttagt ttgcggtatt ccccgagagt gacagtaatt tccgcgccaa
     4201 gcaagacgat acaccatgtc cagtagaccc aaacaaagag aatggggatc accgccagca
     4261 caccgtaaat gagctgatat gacgggaaca tggtgatata aagcgcgaaa cctttctttc
     4321 ctgcttcgaa cagaagtgcg gcgacaaacg cgccgacaat cgcgtcgcgg ttaggtacgc
     4381 ggatggtagg aacaatgctg tacagcaacc agaaggagat ccacgacaac agcaacggaa
     4441 aaatacgcag cacgttgtcg atgacagtat tgagatcgct cgcccagcgc agggagagca
     4501 aataggaact gatcgccaga ctggcccctg ccagcagcgg ccccagcgtt aaaatcatcc
     4561 agtacacggc gaacgagtaa atttttggtc gcgctcgttt actgcgccag atggtattca
     4621 acgcgctatc gatggagtac atcaacaata acgcggtgac gatcagcccg cacgccccaa
     4681 cggcggtcat cttgttggaa ttagcaacaa attgttcgat ataccgctga ataacatcgc
     4741 cagtagcagg cagaaagttg gcaaaaataa agtgacgtaa ctgaatgctg acgtcggaaa
     4801 acatgggaaa agcggcgaaa agcgcaaaaa caacggcaac cagcggcact aatgagagca
     4861 acgacacata ggcaaggtta cctgccaggg ttgtcatgtt gtcctcatca atacgttgcc
     4921 agagtagttt tagccaggcc catagtggac gggtacggtg cctggctttg tcctgaatgg
     4981 tttttagcat aacaccttcg cgaaatagtc cgggatggtg gttttatctt tcaccagaat
     5041 actggtaatg cctagctgat tagctccttc tatattatcg gcgttatcgt cgaaaaagac
     5101 cgtatcgctg ggtgaaaaac cttccgcctg caaaacatgc tggtaaattc gtgcttcagg
     5161 tttgcgcatc cccagatctt gcgacagata gatatggtca gcagcatcac gaatttccgg
     5221 gtattcttcc ggccagaagg tggtatgcag gcggttggta ttggaaagca ccaccacgcg
     5281 atgcccctgc tcacgcagtt tatgcatgat ggcgatcact tccgggcgca gcgcaacaaa
     5341 caccgcctgc cagccgtgag agaactgctc gtagcttagc ggtagagcca tctcatgaca
     5401 cagcgcctct gcgaacgctt cgtcgctaat ttccccacgc tcatgctgat gaaacgcctc
     5461 ccccatatga aaactcttct taagcgatgc cagcggaata cgcgttaaat cgctccaggc
     5521 tcccagcaca cggttaaagt cgatatcgac aatcacatta cctaaatcaa agatatagag
     5581 catttttgcc tcctttcgcg ccacaagaaa ttaactgtag cgggaaagga tgggtttggc
     5641 tatgtggcaa ggtatgaagc caggttaacg gtcacaaaaa tctacgcgtc ttcctgggga
     5701 agaatgattt caatttggct gtcttgtaag cgtgatttta gcgcagcggg cggcagctcg
     5761 tcggtaaaga gcgcagtgac ctgtgccacg ttaccaattt caaccgccgc cgaagcatga
     5821 tacttagtgt gatcggcgac cagcagaata tttctcgcgt gcgccatcat cgttttcacc
     5881 acgttagctt cgtttacatc aaactccatc aacgcgccat cgctctcaat cgccccaacg
     5941 cttgttacca gataatcagc acgaaaatca gccacaaagg acgccgctga agggccaatg
     6001 atcccgctat tatgagagcg caacgtaccg ccgggcacca tcacttcaaa gcgcgggttg
     6061 tggtaaagaa tatgcgccac acgcaggctg ttggtgatta tccgcaaatg attatggtta
     6121 agtaacgccc gggcaacatg ctcaacagtc gtaccaatgg tgataaatat tgttgaacca
     6181 tcaggaatat agtctgccac cgcttcggca atcgcttttt ttttctcggt ttgcgaaacc
     6241 tcacgctgct cgaacgccgt attaacgacg ctggaagccc gacccgcgcc accgtgatgg
     6301 cgcgtaatca ggccttgctc gcttaattta cgaatatccc ggcggaccgt ctgcgtggag
     6361 acatccagca gatttgccag ctcatcaata ttcatatacc cacgctcggc gatcagcatg
     6421 aggagttggt cgtgccgcgg gttaccggtt agttcggtaa ggctcatgaa aattcctcga
     6481 aaaaccatca ccctggcatt ttatacaaaa agtgacaaaa aagatcgggt ttgatcacag
     6541 tcagggatcc cggctcgtcc accgggacgt gtgcatttta acgccgctac accgctggcg
     6601 aagcggactg actccgctaa atccccactt gttgccagcg ccaccgccaa agcgccgtga
     6661 aaaacatcac ccgcacctgt ggtatctacc acatcaactt tgaaggccgg ttgatgctga
     6721 cgcccaccat tttccagcca gtcgcagcct gcgctaccct gggtcacata gacatgacca
     6781 tttgtgagcg tttgtgcctg ttttagcgca ctggccatct ctttcacgcc cgttaagcgc
     6841 gccagacccg gttctgaaaa ggccgcgtga tcgcttaatg ccaccagctc actgatatcc
     6901 tgcggcgtaa tgtccccgtc cagaacggtc atcacacccg cctgacgggc cagggtgaag
     6961 gcttttttag cgccgtcgtg ccagcgtaca tctgccagca caacatccca ctgagagaaa
     7021 tcaatttcct ccaaccactc tgcgtcaggc agcagatccg ggctggggta gttaatgatt
     7081 atccgctcgc ctttggtatc caccatgatg gcggattgcg aagatttcgc ctggttatac
     7141 cgtttggtgt aacgggtgtt aacgccccag gattccagct ctgctagcag gctgttgccc
     7201 gcgtcgtcat cacctacgcg accaataaaa tcgacctgcg cccccagcct tgccgccgca
     7261 accgctgccg tcgccgctgg cccgccacca acttccgtat aatttctcgc cacgtattta
     7321 ccgctctccg tcggtaaccc ttccacgtaa tagatgcgat ccatcacggt tatacctaca
     7381 caagcaacac gaatcatggt cattccttaa acattttggg atgcgttcat tttcatttca
     7441 ccaattgttt aaaaagtgac ggctgtcaat tttttgacga taaattacaa atgcgatcaa
     7501 aaacagacaa gcaaagcatg tcataaatga caaaaagtga catcactgta ttcaggagag
     7561 ggttatggca gcaatcgcgt ttatcggttt aggacaaatg ggttcgccaa tggcgagcaa
     7621 tttattgcag caagggcatc aacttcgcgt cttcgatgtg aacgccgaag ctgtgcggca
     7681 tctggtagac aaaggtgcga ctcccgccgc caacccggtg caggccgcta aagatgccga
     7741 atttatcatt accatgttgc cgaatggcga tctggtgcgc aacgtgttgt tcggtgaaaa
     7801 cggcgtttgc gaaggcttat ctaccgatgc gctggtcatt gatatgtcca ccatccatcc
     7861 gctgcaaacc gataaattga ttgccgatat gcaagccaaa ggcttcagca tgatggatgt
     7921 tccggtaggc cgtacttctg caaatgccat taccggtact ctgttactgc tggctggcgg
     7981 caccgctgaa caagttgaac gtgccacacc gatcctgatg gcgatgggca gtgagttgat
     8041 caacgctggc ggtccgggca tggggatccg cgttaagctc atcaacaact acatgagcat
     8101 cgcgctcaat gcgctttcgg cagaagccgc cgttttgtgc gaagccctga atcttccctt
     8161 cgatgttgcc gtcaaagtga tgagcggtac cgccgccggt aaaggtcact tcaccacttc
     8221 ctggccaaac aaagtcctca gcggagatct ttctcccgcc ttcatgatcg atcttgccca
     8281 taaggatctt ggcatcgccc ttgatgtcgc caaccagctg catgtgccaa tgccgctggg
     8341 ggccgcctca cgggaggttt atagccaggc gcgcgcagcg ggtcgcggtc gccaggactg
     8401 gtccgccatt ctggaacagg tccgtgtcag tgccgggatg actgccaaag taaaaatgta
     8461 acgactggat aaaggaataa atgaatgaat aagtacacca tcaacgacat tacgcgcgca
     8521 tcgggcggtt ttgccatgct ggcggtcgat cagcgcgaag ccatgcgcat gatgtttgcc
     8581 gcggctgggg cacccgctcc ggtagccgat agcgttttaa ctgatttcaa agttaacgct
     8641 gcaaaggccc tctcgcctta tgcctcggcg attctggtag atcaacaatt ctgctaccgc
     8701 caggtggttg agcaaaacgc gattgccaaa agttgcgcca tgattgtcgc cgccgatgag
     8761 ttcattcctg gcaacggtat tccggtcgat agcgtggtta ttgaccgcaa aatcaatccg
     8821 ctacagatca aacaagacgg tggcaaagcc ttaaaactgc tggtgctgtg gcgtagtgat
     8881 gaagatgcgc agcaacgtct ggatatggtg aaagagttca acggactgtg ccactcacac
     8941 ggtctggtaa gcatcattga gccagtcgtc cgcccaccgc gtcgtggcga taaattcgat
     9001 cgcgaacaag cggtcatcga tgccgccaaa gagctgggcg acagtggcgc tgacctctac
     9061 aaagttgaaa tgccgcttta tggcaaaggt ccgcaacaag agcttctctg tgcttcacaa
     9121 cgtctgaatg accatatcaa tatgccatgg gtgatcctct cttccagtgt cgacgaaaaa
     9181 ctgttcccgc gtgccgtacg cgtggcaatg acggcgggcg catcgggatt cctggcaggc
     9241 cgtgcagtct gggcatcggt cgtcggttta ccagacaacg agctgatgct ccgtgacgtt
     9301 tgcgcaccga aattacaaca acctggcgat atcgtcgacg aaatgatggc taaacgccgc
     9361 taagatgagg ataaaaggaa tgaaatggtt taacacccta agccacaacc gttggctgga
     9421 acaggaaacc gaccgcatct ttgattttgg taaaaattcc gtagtgccga ctggttttgg
     9481 ctggttaggc aataaagggc aaatcaaaga agagatgggc acccatctgt ggatcacagc
     9541 tcgtatgttg cacgtttatt ccgttgctgc ggcgatgggt cgacctggcg cttacgcgtt
     9601 ggttgatcac ggtattaaag ccatgaacgg cgcactgcgt gataaaaaat atggcggctg
     9661 gtatgcctgc gtgaatgacg agggcgtggt ggatgcctcc aaacagggct atcaacactt
     9721 ctttgctctg ctgggtgccg ccagcgccgt cacaacgggt cacccggaag cgcgcaagct
     9781 gttcgattac accattgaga ttatcgagaa atatttctgg agcgaagaag agcagatgtg
     9841 cctggaatcc tgggacgaag ccttcagcaa aaccgaagag taccgcggcg gcaatgccaa
     9901 tatgcacgcg gtggaagctt tcttgattgt ttatgacgtc actcacgaca aaaaatggct
     9961 ggatcgcgcg attcgcgtgg cttccgtgat tatccacgac gtcgccagaa ataatcatta
    10021 tcgcgttaac gaacacttcg atacccagtg ggatccgctg ccggattaca acaaagataa
    10081 cccggcgcat cgcttccgcg cgttcggagg tacaccaggc cactggatcg aatggggccg
    10141 tttaatgctg cacatccatg ctgccctgga agcccgttgc gaacaaccac cagcatggct
    10201 gctagaagat gccaaaggtc tgtttaacgc caccgtgcgc gatgcctggg cacccgatgg
    10261 cgcggacggg attgtttata ccgttgactg ggaaggaaaa ccggtggtcc gcgaacgtgt
    10321 acgttggcct atcgtcgaag caatgggtac ggcctacgcg ctctacaccg tcactggcga
    10381 tcgccagtat gaaacctggt atcaaacatg gtgggagtac tgcattaagt acctgatgga
    10441 ctatgaaaat ggttcctggt ggcaggagct ggatgcggac aataaggtca taaccaaagt
    10501 ctgggacggc aaacaggata tttatcacct gctgcattgc ctggtgatcc cgcgtatccc
    10561 gttagcgccc ggcatggctc cagcggttgc agcgggtctg ctggatatta atgcgaaata
    10621 actgatctga aaatagggtg cgggttttcc cctcacccta accctctccc caaaggggcg
    10681 aggggaccgt ccggtgcagt ttttgggttt cacatcagcc tcacgggagg ctgtatgtcg
    10741 ttaattaagg tgccttgtat gcaaatcacc aatatgcatt gcagtggaca gaccgtttca
    10801 cttgccgcgg gggattatca cgcgaccatt gtgaccgtcg gtgctggtct ggctgaatta
    10861 acctttcagg gttgtcattt agtcatcccg cacaaacccg aagagatgcc gctggcgcat
    10921 ctgggtaaag tgcttattcc gtggccgaac cggatcgcca acggctgtta ccgttatcag
    10981 gggcaggaat atcagttacc cattaatgaa catagttcaa aagccgctat tcacgggctg
    11041 cttgcctggc gagactggca aattagtgaa ttaaccacca ccagcgttac cctgacagct
    11101 tttctgccgc ccagttacgg ctatccgttt atgctggctt cacaagttgt ttattcgctc
    11161 aacgcacaca cgggtctttc cgttgagatc gcaagccaga atatcggcac tgtcgcagcg
    11221 ccttatggcg tgggtattca tccttattta acctgcaact taacatctgt tgatgaatat
    11281 ctgttccagc ttccggcaaa ccaggtttat gccgtcgatg aacacgtaaa tccgacaacg
    11341 cttcaccacg ttgatgagct ggatttaaat ttcacgcagg cgaaaaaaat agccgcgaca
    11401 aaaatcgacc acacctttaa aaccgctaat gatttgtggg aaatgaccat tacccatcca
    11461 cagcaggcgc tttcggtaag cctttgttcc gatcaaccct gggtgcaggt ttatagcggt
    11521 gaaaaattac agcgtcaggg actggcagtt gaacctatga gttgcccgcc taacgccttt
    11581 aattcaggta tcgacttatt attactggaa ccaggaaaac cgcaccgcct gttttttaat
    11641 atttacggac agagaaaata acttgtattg attagcagtc aaaatcattg aagaacattt
    11701 cactcgctaa aggcaacatt atgtatcttc aatttattaa gttaaaaaca gctcagcttc
    11761 tgtaaagttt ttatatatca gttttatgag aatcatttta cataagcctg taataacagg
    11821 aaggcaacaa tctcgttctc aactctatgg agattagtta tggatacgcc acgtccacag
    11881 ttattagatt ttcaatttca tcagaataac gacagtttta ccctacattt tcaacaacgt
    11941 cttattttaa cccatagcaa agataatcct tgtttatgga ttggctcagg tatagcggat
    12001 atcgatatgt tccgcggtaa tttcagcatt aaagataaac tacaggagaa aattgcgctt
    12061 accgacgcca tcgtcagcca gtcaccggat ggttggttaa ttcatttcag ccgtggttct
    12121 gacattagcg ccacgctgaa tatctctgcc gacgatcagg gccgtttatt gctggaacta
    12181 caaaacgaca accttaacca caaccgtatc tggctgcgcc ttgccgctca accagaggac
    12241 catatctacg gctgcggcga acagttttcc tacttcgatc tgcgcggcaa accgttcccg
    12301 ctatggacca gtgaacaagg cgttggtcgc aacaaacaaa cctatgtcac ctggcaggcc
    12361 gactgcaaag aaaatgcggg cggcgactat tactggactt tcttcccaca gcctacgttt
    12421 gtcagcacgc agaagtatta ctgccatgtt gataacagtt gctacatgaa cttcgacttt
    12481 agcgccccgg aataccatga actggcgctg tgggaagaca aagctacgct acgttttgaa
    12541 tgtgctgaca catacatctc cctgctggaa aaattaactg ccctgctggg acgccagcca
    12601 gaactgcccg actggattta tgacggggta acgctcggca ttcagggcgg gacggaagtg
    12661 tgccagaaga aactggacac catgcgtaac gcgggcgtga aggttaacgg catctgggcg
    12721 caggactggt ctgggatccg tataacctct tttggcaagc gcgtgatgtg gaactggaag
    12781 tggaacagcg aaaactaccc gcaactggat tcacgcatta agcagtggaa tcaggagggc
    12841 gtgcagttcc tggcctatat caacccgtat gttgccagcg ataaagatct ctgcgaagaa
    12901 gcggcacaac acggctatct agcaaaagat gcctctggcg gtgactatct ggtggagttt
    12961 ggcgagtttt acggcggcgt tgtcgatctc actaatccag aagcctacgc ctggttcaag
    13021 gaagtgatca aaaagaacat gattgaactc ggctgcggcg gctggatggc tgacttcggc
    13081 gagtatctgc ccaccgacac gtacctgcat aacggcgtca gtgccgaaat tatgcataac
    13141 gcctagcctg cgctgtgggc gaagtgtaac tacgaagccc ttgaagaaac aggcaagctc
    13201 ggcgagatcc tcttctttat gcgcgccggt tctaccggta gccagaaata ctccaccatg
    13261 atgtgggcgg gcgaccagaa cgtggactgg agtctcgacg atggcctggc gtccgtagtc
    13321 ccgacggcgc tgtcgctggc aatgaccgga catggtctgc atcacagcga cattggcggt
    13381 tacaccaccc tgtttgagat gaagcgcagc aaagagctgc tgctgcgctg gtgcgatttc
    13441 agcgccttca cgccgatgat gcgcacccac gaaggtaacc gtcctggcga caactggcag
    13501 tttgacggcg atgcggaaac tatcgcccat tttgcccgta tgactaccgt tttcaccacc
    13561 ctgaaacctt atctgaaaga ggccgtcgcg ctgaatgcga agtccggtct gccggtcatg
    13621 cgcccgctgt tcctgcatta cgaagacgat gcacatacct attccctgaa atatcagtac
    13681 ctgttaggcc gcgacattct ggtcgctccg gtacacgaag aaggtcgcag cgactggacg
    13741 ctctatctgt cggaggataa ctgggtacat gcctggacgg gtgaagcgtt ccggggcggg
    13801 gaagttaccg ttaatgcgcc catcggcaag ccgccggtct tttatcgcgc cgatagcgaa
    13861 tgggcggcac tgttcgcgtc gttaaacagc atctaatcac atccgcccgt ggggaaactc
    13921 atgggcaaag ggagaacatc gatgagtcac atcacaacgg aagatccagc aactttacgc
    13981 ctgcccttta aagagaaact ctcttacggt attggcgacc tggcctctaa catcctgctg
    14041 gatatcggta cgctttatct tttgaagttt tataccgacg ttctggggct gccaggcacc
    14101 tatggcggca ttatcttttt gatttcaaaa ttctttactg cgtttaccga tatgggtacc
    14161 ggcattatgt tggattcccg acgcaagatc ggtccaaaag gtaagttccg tccttttatt
    14221 ctgtatgcgt cattcccggt caccttactg gcgatcgcca actttgtcgg cacaccgttt
    14281 gatgtcaccg gtaaaacggt gatggccact attctgttta tgctttacgg actgtttttc
    14341 agcatgatga actgctccta cggcgcaatg gtcccggcaa tcaccaaaaa ccccaacgaa
    14401 cgcgcctcac tggcggcatg gcgtcagggc ggcgctacgc tgggcctgct gctgtgcacg
    14461 gtgggattcg tgccagttat gaatcttatc gaaggtaatc agaaacttgg ctatatcttc
    14521 gccgccacgc tgttttcact gttcggcctg ctgtttatgt ggatctgcta ctcaggcgtg
    14581 aaagagcgtt atgtcgaaac ccaaccaacc aatccggcgc aaaagcctgg cctgttgcaa
    14641 tctttccgcg caattgccgg taaccgccca ctgttcattc tgtgtattgc caacctctgc
    14701 accttagggg cgtttaacgt caagctcgcc attcaggtct attacaccca gtacgtactt
    14761 aacgatccca tcctgttgtc gtatatggga tttttcagca tgggctgtat tttcatcggc
    14821 gtgttcctga tgcctggcgc agtcagacgt tttggtaaga agaaggtcta tatcggcggc
    14881 ctgctgattt gggtgctggg cgatctgctc aactatttct tcggcggcgg ctcggtcagc
    14941 tttgtggcgt tctcctgcct ggcgttcttc ggctcagcgt ttgttaacag cctgaactgg
    15001 gcgctggttt ccgacaccgt cgagtacggc gagtggcgca ccggcgtgcg ttcggaaggc
    15061 acgctctaca ccggcttcac cttctttcgc aaagtgtctc aggcgctggc tggtttcttc
    15121 cccggctgga tgctgacgca aattggctat gtgccgaacg tcgcacaggc tgaccacacc
    15181 attgaagggt tacgccagtt gatcttcatc tacccaagcg cactggcggt agtcaccatt
    15241 gtggcgatgg gttgcttcta cagcctgaac gagaagatgt atgtccgcat tgtggaagag
    15301 atagaagccc gtaaacgcac ggcgtaataa taataaacga cgccctgcgg ggcgttataa
    15361 ggagtgatta tgtctgacca taatccactg acattaaaac tgaatctgcg ggaaaaaatc
    15421 gcctatggta tgggcgacgt cggttcgaat ttaatgctct gcatcggtac tctgtatctc
    15481 ctcaaacttt ataccgatga attagggatg cctgcttact atggcgggat tatctttctg
    15541 gtcgcgaagt tttttaccgc gtttaccgat atgctcaccg gatttttact cgactcgcgg
    15601 aaaaatattg ggcctaaagg caaatttcgt ccctttattt tatatgcagc agttccggcg
    15661 gcgttaattg caacgctcca gtttatcgcc accacctttt gtttgccggt taaaacgacg
    15721 attgccaccg cgctgtttat gatgtttgga ctttcataca gtctgatgaa ctgctcgtat
    15781 ggtgcgatga tcccggcaat taccaaaaac ccgaatgagc gcgcgcagct cgcggcttac
    15841 cgccagggtg gtgcaaccat agggctattg atttgtaccg tggcgtttat tccgttgcag
    15901 tcgctttttt ctgactcaac cgtcggttat gcctgtgcgg cacttatgtt ctccattggc
    15961 ggctttattt ttatgatgct gtgctacaga ggcgtcaaag agcattatgt ggacacagcg
    16021 ccaaccggac ataaagccag tattctcaaa tctttttgcg cgatatttcg taatccacca
    16081 ttgctggttt tatgcattgc taacctgtgt actctggcgg catttaatat caaactggcg
    16141 attcaggtct attacaccca gtatgtgctg aatgatatta atttattgtc gtggatggga
    16201 ttcttcagta tgggatgcat cctcgtcggc gtattactgg tgccagtaac tgtaaaatgt
    16261 tttggtaaaa aacaggttta tttagctggc atggtgctat gggcggtggg tgatatactg
    16321 aattatttct ggggaagtaa ctctttcact ttcgtcatgt tctcttgtgt cgcctttttt
    16381 ggcacggcct ttgtaaacag cctgaactgg gcactggtac cagataccgt agattacggt
    16441 gaatggaaaa ccggtattcg tgccgaaggt tctgtttata ccggttatac cttctttcgt
    16501 aaaatttctg ccgcacttgc tggcttcttg ccaggcatta tgctgacgca aattggttat
    16561 gttcccaaca tcgcgcaaag cgatgcgaca ttacagggtt tgcgtcagct catttttatc
    16621 tggccttgtg cactggcaat tattgctgca ttgacgatgg gattctttta cacactcaat
    16681 gaaaaacggt ttgcattgat tattgaggaa atcaaccaac gcaaaaataa agagatggcg
    16741 acagaaaaaa aaacggcttc cgtaacgtta taaatgtcaa tagccgctat ttccatcctg
    16801 gtgggtggcg gcctccctac gtttaaaaaa tggacttatt atgaaaaaga ttaatgcaat
    16861 aattttatta tcttctctga cctctgcctc ggtatttgct ggcgcatacg tcgaaaacag
    16921 ggaggcatat aatcttgcct ccgatcaggg cgaggttatg ctacgtgtgg gttataactt
    16981 cgatatgggc gcgggtatta tgttaaccaa tacctacacc tttcagcgag aagatgaact
    17041 aaaacatggc tataatgaaa ttgaaggttg gtatccttta ttcaaaccaa ccgataaatt
    17101 aaccatccag cccggtggct taat
//
LOCUS       DAETPZ010000082        16903 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.082, whole genome shotgun sequence.
ACCESSION   DAETPZ010000082 DAETPZ010000000
VERSION     DAETPZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.082"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..882)
                     /locus_tag="K0W59_003066"
     CDS             complement(<1..882)
                     /locus_tag="K0W59_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBI3024008.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGA"
     gene            989..1285
                     /locus_tag="K0W59_003067"
     CDS             989..1285
                     /locus_tag="K0W59_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3024009.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1419..1844)
                     /gene="arsC"
                     /locus_tag="K0W59_003068"
     CDS             complement(1419..1844)
                     /gene="arsC"
                     /locus_tag="K0W59_003068"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HBI3024010.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1857..3146)
                     /gene="arsB"
                     /locus_tag="K0W59_003069"
     CDS             complement(1857..3146)
                     /gene="arsB"
                     /locus_tag="K0W59_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HBI3024011.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3200..3553)
                     /gene="arsR"
                     /locus_tag="K0W59_003070"
     CDS             complement(3200..3553)
                     /gene="arsR"
                     /locus_tag="K0W59_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HBI3024012.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            4297..4380
                     /locus_tag="K0W59_003071"
     CDS             4297..4380
                     /locus_tag="K0W59_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HBI3024013.1"
                     /translation="MIDKAIIVLGALIVLLELIRFLLQLLN"
     gene            complement(4434..5786)
                     /gene="gorA"
                     /locus_tag="K0W59_003072"
     CDS             complement(4434..5786)
                     /gene="gorA"
                     /locus_tag="K0W59_003072"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBI3024014.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5858..6700)
                     /gene="rlmJ"
                     /locus_tag="K0W59_003073"
     CDS             complement(5858..6700)
                     /gene="rlmJ"
                     /locus_tag="K0W59_003073"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HBI3024015.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6903..8945
                     /gene="prlC"
                     /locus_tag="K0W59_003074"
     CDS             6903..8945
                     /gene="prlC"
                     /locus_tag="K0W59_003074"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HBI3024016.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADILAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8953..9705
                     /gene="rsmJ"
                     /locus_tag="K0W59_003075"
     CDS             8953..9705
                     /gene="rsmJ"
                     /locus_tag="K0W59_003075"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HBI3024017.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9754..11223)
                     /gene="dtpB"
                     /locus_tag="K0W59_003076"
                     /pseudo
     CDS             complement(9754..11223)
                     /gene="dtpB"
                     /locus_tag="K0W59_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(11529..11963)
                     /gene="uspA"
                     /locus_tag="K0W59_003077"
     CDS             complement(11529..11963)
                     /gene="uspA"
                     /locus_tag="K0W59_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HBI3024018.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12354..12689
                     /gene="uspB"
                     /locus_tag="K0W59_003078"
     CDS             12354..12689
                     /gene="uspB"
                     /locus_tag="K0W59_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HBI3024019.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12760..14259)
                     /gene="pitA"
                     /locus_tag="K0W59_003079"
     CDS             complement(12760..14259)
                     /gene="pitA"
                     /locus_tag="K0W59_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HBI3024020.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            14491..15690
                     /locus_tag="K0W59_003080"
     CDS             14491..15690
                     /locus_tag="K0W59_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBI3024021.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         4301 a         4219 c         4363 g         4020 t
ORIGIN      
        1 tgcgccggca ctgccgatga ttaatgccat taatgctccc agacccattc cttttgtcat
       61 cagaacagat gccagaggga taaccgcctc agcccggata tacagcggaa taccgacaac
      121 ggcgcttaat gggatggcga agggattatc tgctcctgca tgagcggcaa tccacgctga
      181 gggaataaag ccataaataa aagatcctat taatactcct aacaaaagat atggcagtac
      241 atctttaaac tgttgcaacg catctttggt ggctagtttt atgggattaa tacttactac
      301 agctttggta ctgcaacagg taactacggg gttctcaatg gctttagccg ctggactaca
      361 acaactcact tttatgggcg tatatgtcgt tcccggcgaa gtttttgctg gagcacaaca
      421 atttgctgat gagcttttac tggcaatgat atgacgttca aaccccagag aatccaggat
      481 aatactggca agaacggaga cgccggctgc gataatcgcg tacaacaagg tgactttcca
      541 accaaaggtc atccacatta gtccgacgat aatgggatta agtaatggtg aaacaaataa
      601 aaaagtgaga gtcggaccaa acccggcttt cgctgacaac aatccacgta acatgggtat
      661 tgtcgagcaa ctacagaacg gggtaacggc tcccaacaga gcagccagga gatagcctct
      721 cccttttctg gcccccatca tctgctggat tttatggtct ggcacctttt gtcttatcag
      781 gctgacaccg gcactaatca ctataaacag caacgaaagc tcgacggcga gaaacacaaa
      841 catctctgcg gcatcttgca gcatcgcaag ccaactactc atacgtaacc tcattggata
      901 aatctatatt tccagaataa tcgaaatgaa attcagatca agatatttct ggtattatcg
      961 aaatatcaac cagacagagg ggtgtcatat gcagttggaa gaagtcgcta aagcgctgaa
     1021 agagctaggt cacccgaccc gtttgttcat atttaaacat cttgtcaaag ccggggaaca
     1081 agggttgccg gtcggtgagt tgcaaaagca gcttgggata cctggctcca cactcagcca
     1141 ccatatatca gcactggtat cggtaggact ggtcaaacaa aaccgtgaga gtcggacgct
     1201 gatgtgtgtt tcgcaatacg caatgctgga agcgataatt gaattcttac gcgaggagtg
     1261 ctgcgtaaat agcaaaattg cctgatgcgc tacgctctac atatttcatg ccatatattg
     1321 aatttgcacg gttttgtagg tcggataaga cgcgttagcg tcgcatccga cataaacaaa
     1381 gcgcacttta ccaactatcg gcagtggata tcgccgcttt atttcaggcg cttacccgct
     1441 tcatcaacca ctttctcgcc atcttccttg gagaatgcgc ctttttgcgc atctggcaga
     1501 atttccagca ccacttctga agggcggcac aggcgagttc ccagcggcgt caccacaatc
     1561 gggcgattaa tcagaatcgg gtgctgaagc ataaagtcga ttaaccgatc gtcagtaaat
     1621 ttatcttccg caaggcccag ctcctcatac ggttcgacgt ttttacgcag cagcgcgcgt
     1681 acggaaatcc ccatatcggc aatgagtttg accagttcat cgcgcgttgg cggagtttcc
     1741 agataatgga taatggtcgg ttctgtgccg ctgttgcgga tcatctccag cgtattacgc
     1801 gacgtgccgc aggccgggtt gtgataaatg gtaatgttgc tcatatcagt atctcattac
     1861 aaagtgaaag agagacgtag cgccagcgca gccagcgtca caaacagcac aggcagggtc
     1921 atgataatcc ctgtacggaa gtaatatccc cagctgatag tcatattctt ctgcgaaagt
     1981 acgtgcagcc agagcagcgt tgccaggcta ccaatcgggg taattttcgg ccccaaatcg
     2041 cagccaatca cgttggcata aaccatcgct tctttgataa cgccagatgc cgtgctgcca
     2101 tcaatggaca acgcgccaac cagtaccgtc ggcatattgt tcataataga agagaggaag
     2161 gcggtgagga atccggtgcc gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg
     2221 agtacgccag aaagatattc cgttaatccg gcattgcgca ggccataaac caccagatac
     2281 atgccgagcg agaagatgac aatctgccag ggggcaccgc gcaggacttt acccgtatta
     2341 atcgcatgac cgcgtttagc gacgacaaat aatatcagtg cgcccacagc tgcaatggcg
     2401 ctcaccggaa tgccgagcgg ttccaggacg aaaaatccca ccagcagaag cagtaaaaca
     2461 acccagccag ttttgaacgt agcaggatct ttgatcgctt ctgcgggaga tttcagcagc
     2521 gccatatcgt agttctgcgg aatatctttg cgaaaataga gatgtagcat caccagcgtg
     2581 gcaacaatcg cggcgatatc caccggcacc atcaccgagg cgtattcgcg aaagccgagg
     2641 ccaaagaaat cagcggaaac gatattcacc aggttggaga caataagcgg caggctggcg
     2701 gtatcggcaa tgaatccggc cgccatcacg aacgccagcg tcgtgccttt actgaatcct
     2761 aaagccagca gcatggcgat gacaatcggc gtcaaaataa gcgccgcgcc atcattggca
     2821 aacagggccg caacggcggc accgagcagg acaatccagg taaacagcaa gcgaccgcgt
     2881 ccattgcccc agcgtgaaac gtgcagcgct gcccattcaa aaaagccgga ctcatccagc
     2941 agcaggctga tgataatgac ggcaataaac gccgccgtcg cgttccagac gatattccac
     3001 accaccggaa tatcacccgg atgaaccacg cccgtaacca acgccagtac cgcgccgagc
     3061 gttgcactcc agccgatgcc taaacctttc ggctgccaga taaccaatac gatggtcagg
     3121 acaaagatag cgcctgccag taacataatg cctcccggat aaaacacatg tgcaaatcca
     3181 tatgtgttta gctaaatttt taactgcaaa tgttcttact gtctccggaa cagttttgtc
     3241 tggccaggtt gcggacaatt gcctgaatct tttcctgttc acatcgccag gcttcatcaa
     3301 taattttcgc cgcccatgct ggaatatgcg gcgataagcg gtaatgaacc cacttacctt
     3361 gcttgcggtc cagcaatagc ccgctttcac gcagcaatgc cagatggcgg gatatcttgg
     3421 gctgcgactg atcgagggca gtgcagagat cgcagacgca taactctccc agttcgctaa
     3481 gcagtaaaac gatgcccaga cgggtttcat cagcaagaat tttgaacaat tggatgggta
     3541 acagaaatga catattgcgc tcctgattgt tgcaggtagt gtctctcttc gaagcggata
     3601 agtcaaaaac atatatgact taacgaatgt gtaagtgcag aggaaggtaa taggtgtgaa
     3661 ttttgagttg gctattcatt tgaaaggcgg tttgaatcag gacgtaaaaa aaccgacttt
     3721 gcgtcggttt ttttactctt cagcctgagt tggtggctct ggctggagtg agaatgccca
     3781 catctaacgg ttcaatacta ttagccagtg gctaacattg caagcgttag cgatttcttc
     3841 agctggagaa gggacagttt gctatacatg gtggtgaaag aggatttata tactttcatc
     3901 gggtcgctgc cgtaccaagc cttgtccgaa tccggacgta aaaaaaccga cttcgcgtcg
     3961 gtttttttac tcttcagccc agagttggtg gctctggctg gagtgagaat gccatcatct
     4021 aacgcatcaa tgctaatacc atactgaaac tattgcaagg ttatgctggt tatttagcct
     4081 aattacactg tatgattatc cagttagctc tgaggcattt tcactctggc aatgcgcata
     4141 aacgctttca aagtcctggt cagaagtacg ggtggtgccg ttaactgatg ctctggccgg
     4201 agtgagattg cccacatcta acaatgaggc atgcgccgtg acaggcagtg gatgagtaag
     4261 cggatgcatt ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt
     4321 cttggggcgt taattgtgct gctggaactg atccgctttc tgcttcagct tctgaactga
     4381 tagcggaaac gtaattaagg gctaagagca cactactctt agccctttaa catttaacgc
     4441 atggtcacga actcttctgc cgccgttggg tgaatggcga cggtattgtc gaagtctttt
     4501 ttggttgcgc ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaag
     4561 ccaatgccgt gaataccgac aatcttctct tctggcccaa cgcacaccag cttcatgcgg
     4621 cacggctggc ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact
     4681 ttcacctgat cgtcgccgta ctgctcgcgc gcctgcggtt ccgttaaacc aacagtacca
     4741 atcggcggat ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc
     4801 ttgttattaa acaggcgttc agagagacgg cgacccgctg caactgccac cggtgtcagt
     4861 tccactgcac ccgtgttatc gcccaccgcg taaatacctt caatattggt gttttgatat
     4921 ttatcgacga cgatatagcc tttttcgttg gttttaacgc cagcggcttc gaggttgatg
     4981 ttgtcattgg caggctcgcg accaatcgcc caaatcaggc aatccaccgt ttcactgcga
     5041 ccatcttcca gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg
     5101 gcgttggtat gcaactgtgg gccttcggcg ttcatcactt cgaccagcgt ttcagagatc
     5161 atcgggtcga agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt tttcgcgccg
     5221 aggccgttaa tcacgccagc cagctcaacg gcgatgtaac ccgcgccaac aaccgccacg
     5281 cgctctggca aagcaggaag ggcgaagaag ccatcggaat caataccgta ttccacgcct
     5341 ggaatatccg ggtggctcgg acgaccgccg gtggcgatca gaatatgatc ggccgtgatg
     5401 gtttcgccgt taacttccag cgttttggca tcaacgaagc gggcaaagcc tttgattaca
     5461 tcaacgttat ttttaccgag cacgttttca taggaagtat gaatacggtc gatataggcg
     5521 gtacggctgg cgatcaacgt ttcccagttg aacttgttga tggtggtatc gaaaccataa
     5581 tcagggccgt acatatggat cgcttcacgg atttgcgccg cgtgccacat cacttttttc
     5641 ggcacgcagc cgacgttcac gcaggtgccg cccagctctt tggcttcaat cagtgcgcat
     5701 ttctggccgt acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg
     5761 gcgatgtaat catagtgttt agtcatgaca tattgtcctt atcgttgatt accgcgattg
     5821 tagcgcgaga cgtaataggt gccagcaatg gctgcaatta ctccggcacg atccagctta
     5881 cggtggcgtg cccggtacct gccggaacca gtttgctgtg cagccacgac agcacgttat
     5941 tcatctgctg ttccagcttc cacggcgggt taatcacaat catgccggaa gcggtcatgc
     6001 cacggcgatc gctgtctggc agcaccgcca gttcaatttg cagaattttg cgaatgccgg
     6061 tcgcttccag gtcgtggatc atgcgcttaa tttgctggcg cagcaccacc ggataccaca
     6121 atgcgtaagt gccagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgcct
     6181 gatagtcggt tttcatttca tacggcgggt cgataaggat taaaccacgg cgagaaaccg
     6241 gcggcagttt ggccttaagt tgctggaaac cgtcggcttt ttcgacgcgt gcacggctgt
     6301 ctttctgaaa tccagaacgc agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca
     6361 ggctgtcttg ttcgcgcagt agctggcgag caatcaacgg cgaaccgggg tagtaacgca
     6421 actggccgct acggttgaag tgttttacca cattgatgta cgcctccagt tctgcgggca
     6481 aatcgtcctg ctgccagata cgggcgatac cttcgagata ttcgccggta cgctcggcat
     6541 gttcgctacc taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt
     6601 tatctttctc tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg
     6661 cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa
     6721 gtaaaaattg gcccgtattc ggggcgcaca aaagcctatc aggacagtat accgaaaaga
     6781 ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct
     6841 acacacatta tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc
     6901 taatgacgaa tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg
     6961 aacatgtcgt tccagccgtg actaaggcat tgaacgactg ccgcgaaaat gtggagcgcg
     7021 tagtagcgca aggggcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg
     7081 acgatgtttt ggggcgtatc ttctccccgg tcagccacct gaactcggtg aaaaatagcc
     7141 cggaactgcg tgaagcgtac gaacaaaccc tgccgctgct gtcggaatac agcacctggg
     7201 tagggcaaca tgaagggctg tataaagcgt accgcgacct gcgcgatggc gatcattacg
     7261 ccacgctgaa cacggcgcag aagaaagcgg ttgataacgc cctgcgtgat tttgaactct
     7321 ctggcatagg tctgccgaaa gagaaacagc agcgttacgg cgaaattgct acccgtcttt
     7381 ctgaactggg caaccagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac
     7441 tcgttaccga cgaagcgggg ctggcgggga tgccagaaag cgcgctggct gcggcaaaag
     7501 cccaggccga agcgaaagag ctggaaggct acctgctgac gctggatatc ccaagctacc
     7561 tgccggtaat gacctactgc gacaaccagg ctctgcgcga agagatgtat cgcgcttaca
     7621 gcacccgcgc ctccgatcaa ggcccgaacg ccggtaagtg ggataacagc aaggtgatgg
     7681 aagagatcct cgcgctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct
     7741 ttaaatccct tgccactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg
     7801 atctggcaaa acgcgcgcgt ccgcaaggcg aaaaagagct ggagcaactg cgtgccttcg
     7861 ctaaagccga atttggtgtc gatgagttgc agccgtggga tatcgcttac tacagcgaaa
     7921 aacagaaaca gcacctctac agcatcagcg acgagcagct gcgtccgtac ttcccggaaa
     7981 acaaagcggt taacggcctg tttgaagtgg tgaaacgtat ttacggcatc accgctaaag
     8041 agcgtaaaga tgttgatgtc tggcatccgg atgtacgttt cttcgaactg tatgacgaaa
     8101 ataacgaact gcgcggcagc ttctacctcg atctgtatgc ccgtgaaaac aagcgcggcg
     8161 gggcgtggat ggatggctgc gtaggccaga tgcgtaaagc tgatggttct ctgcaaaaac
     8221 cggtcgcgta tttgacttgt aacttcaacc gcccggtaaa tggtaaaccg gcgctgttca
     8281 ctcacgacga agtgatcacc ctgttccacg agttcggtca cggcctgcac catatgctga
     8341 cccgcatcga aaccgctggt gtttccggta tcagcggtgt gccgtgggat gcggtcgaac
     8401 tgccgagtca gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctctg
     8461 gtcactatga aaccggcgaa ccgctgccga aagagttgct ggataaaatg ctggcggcga
     8521 agaactacca ggcggcgctg tttattctgc gtcagctgga gttcggcctg tttgatttcc
     8581 gccttcatgc cgagttccgc ccggatcagg gggcaaaaat cctcgaaact ctggcagaaa
     8641 tcaagaaact ggttgccgtg gtgccatctc cgtcctgggg ccgtttcccg cacgctttca
     8701 gccatatttt cgccggtggt tatgcggcag gttactacag ctacctgtgg gctgacatac
     8761 tggcggcaga tgctttctcg cgctttgagg aagagggcat tttcaaccgt gaaaccgggc
     8821 agtcgttcct cgacaacatt ctgagccgtg gcggttcaga agagccgatg gatctgttca
     8881 aacgcttccg tggtcgtgaa ccgcagctgg atgcgatgct ggagcattac ggcattaagg
     8941 gctgatcatt cagtgaaaat ctgcttaatt gatgaaacag gcgccggaga cggcgcctta
     9001 tctgttctgg cggcccgctg ggggctggag cacgatgaag acaacctgat ggcgctggtg
     9061 ttaacgccgg aacatctgga attgcgcaag cgtgatgagc caaaacttgg cggcatcttt
     9121 gttgattttg ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag
     9181 gcggtggcga aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgccacc
     9241 gcagggctgg ggcgcgatgc ctttgtactg gcttcagtcg gctgccgcgt gcggatgctg
     9301 gagcgtaatc cagtggttgc cgcgctactc gacgacggcc tggcgcgtgg ttatgcggat
     9361 gcggaaatcg gcggctggtt gcaggagcgg ttgcagttaa ttcacgcctc cagcctgacg
     9421 gcgctaaccg atattacccc acgcccgcag gtggtctatc tcgatccgat gttcccgcat
     9481 aagcagaaaa gcgcgctggt gaaaaaagag atgcgtgtct ttcagtcgct ggtggggccg
     9541 gatcttgatg ccgatggatt actggagcct gcacgcttgc tggcgaccaa acgcgtggtg
     9601 gtaaagcgcc cggactacgc gccaccgctg gcgaatgtcg ccacgccaaa cgcggttgtg
     9661 actaaagggc atcgctttga tatctatgca ggtacgccag tgtaaaagta aagcagggta
     9721 gcgataacgc taccctgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat
     9781 gcgtttcagc cacggcacca tcaacagcat cactactgca acgcccagcg tgaccagacc
     9841 aatcttacca aacacgttgg tatagacggg caacgtctca agcggatcgg taatgttgtc
     9901 cggcaccgcg gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag
     9961 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
    10021 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
    10081 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagtcgt
    10141 caaaaagccc agtgagcaca taaacatgcc gagagtaaat ttcatcggca tcgagaggtc
    10201 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
    10261 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
    10321 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
    10381 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
    10441 atccagcttg aatgcctgac gaaagaagat gatggtgacg acgatggaga gaacaatcag
    10501 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
    10561 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
    10621 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
    10681 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
    10741 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
    10801 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
    10861 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
    10921 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
    10981 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
    11041 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
    11101 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
    11161 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
    11221 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
    11281 gaaaaagttt acatctgtca gagattctta atattgatgc ggtgaataga ttaatacact
    11341 attacaacag aaaataacca gctgatcaca ctaatgatgg tgttaaattt tgtattggca
    11401 ggagattttg attgcgtacg cagatcgtag gccagataag gggtttacgc tgcatcaggt
    11461 aacaaggcgc gccaaaaagc aaaacgcccg catatgcggg cgttcaggaa cacgtcgtgg
    11521 agggatgatt attcttcttc gtcgcgcagc ggaacaatca gcatatcaac gtgaacggtg
    11581 ttgatcagct gacgtgcgga agacatcagt ttgctccaga agtcctggtg gtgaccacaa
    11641 accaccagat ccatatcgta tttcttgatt gcatcgacca gaacctgacc caggtcgccg
    11701 ctgccgctca gggtttcagt gattggatag cctgcattag tggaaagctc ggtcagcgca
    11761 tgatgtgtct cttcagagat gcgtttctgc atatcaccca gattcacatc aataagcccg
    11821 gtgtataggt cagagtagtt tacatctacg tggatcagag aaactttcgc attgtagggg
    11881 cgagccatag agactgcttt ctctaccaga actttgcttt ccggggagag atcgactgcg
    11941 atgagaatgt gtttataagc catagtgtta ctccttccat aaagttgtcg atgactggcc
    12001 agctagcgtt tcttgtgccg ttacgtcacc cgcgtcctgc gaactacgtg cgcggggcta
    12061 acccagccgt aaagattcag tgttaagacc atccttacct tatagcgacc cggatgatcc
    12121 gtcaatccgc cttgcttacc cgttagataa acaaattttc gacatgaata tattgatagt
    12181 ggttaacctt ctggaaaaaa aacaacctga tctcctacac tatctataga gccgctcgga
    12241 tgttggtcac cacaaaagcg gtttctccag gacctggtgt taactgattg gcagcgtatc
    12301 gggagcggta gccccggggt ggaactccgc gggcaggtcg ccggggagga ggtatgataa
    12361 gcaccgtcgc attattttgg gctttatgtg tcgtttgcat tgttaacatg gcgcgctatt
    12421 tctcatcact acgcgcgttg ttagtggtac tgcgtaactg cgatccattg ctctatcaat
    12481 atgttgatgg agggggcttt tttacctcac atggccaacc caacaaacag gtgcgtctcg
    12541 tttggtatat ctatgcccaa cgttatcgcg atcatcacga tgatgagttt attcgccgct
    12601 gtgagcgggt gcgtcggcag tttattctga ctagcgcatt gtgtggtctg gtggtggtca
    12661 gcctgattgc attgatgatt tggcattaag cacttcagca ataataacgc ataaaaaaag
    12721 cgggccagta aactgacccg ctcgttttgg tgcgtacgat tacaggaact gcaaggagaa
    12781 ccagtacagc ccgccggaaa gtagtaccgc agccggaagg gtaaacaccc aagccatcag
    12841 aatactggtt acggttttac gctgtaagcc gccgccatct accaccatcg tccccgcgac
    12901 agaagaggag agtacgtgag tggtggaaac cggcatcccg gtataactcg ccaggccgat
    12961 tgacactgcc gccgtcatct gggcagacat cccctgagcg taggtcatgc ctttcttacc
    13021 gattttctca ccgatagtcg ttgccacacg acgccagcca atcatcgtac cgatacctaa
    13081 cgccagcgcg accgccatga tgatccacac cggcgcatac tcgatggtgc taagcatgtc
    13141 ggacttcagt ttcttcaaca ggcgctgatc gtcagcactc acaccaggca tcttcaccac
    13201 tttgtcgata gtgtcagaaa cgcacagcat aatgcggcgc atctgactac gttgatcaag
    13261 cgacagcttg tcgtagcttt ccacatcggt ggtcaacata cctttcaggc ggttgagcgc
    13321 gttaatggta ttcgacggat ggcagtggaa ctccgcaggt tgcgttgcgc cagcttccgg
    13381 agccggtact aactgatcag caccggtagc ctgtttgagc agcgcaggat gctgctcaaa
    13441 gtaagcttcg acgttgttga tggcatcacg ggtacgggtg atttcgtagc cagtggcatt
    13501 catgttcacc acgaagcctg ctggcgcgac gccaatcaat accaacataa ccagaccaat
    13561 gcctttctga ccatcgttcg cgccgtgcga aaacgccacg ccgatagcgg aaaggatcag
    13621 cgcgatacgc gtccagaacg gcggcttttt cttgccgtct ttcttttcac gctccgctgg
    13681 ggtcaggtgg atacgggcgc gtttcttggt gccgctccag tagcgacgca gcaagaaaat
    13741 cagaccgcca gcaaacacca ggccgacaat aggggaaacg atcagagaac cgaaaatact
    13801 taatactttc gggatattga gtgcatccac cactgacgtc ccggtcatca acgcattggt
    13861 taaaccaatc ccgatgatcg cgccaatcag cgtatgagag ctggatgcag gtaaaccaaa
    13921 gtaccaggta cccaggttcc agataatcgc cgccagcaac atagagaaca ccatggcaag
    13981 gccatgagac gatcccatat taagcagcag atccgtcggc agcatatgca caatggcata
    14041 ggcaacactc agaccaccca gcaaaacacc caaaaagttg aacaccgccg ccataaccac
    14101 ggcgagctga gaacgcatcg cgcgggtata gataacggtt gccacggcgt tggctgtgtc
    14161 atggaaacca ttgatggctt cgtagaacag cacaaaagcc agtgcaagca ataataacag
    14221 cccggtatgc aaatccaggc cagcaaacaa atgtagcata ggacgttacg ccattttgag
    14281 gacatgaacg cggcgcatta tcagtgactt tcgcggcgcg ggcaaagtga aatatagact
    14341 ttttttgatt tgcctcctgt atggatttca ctcaaaaaat aattatctta tataattcag
    14401 gcaaatactt ccttttagta atattgatgc tggtgcgacc actgaggaat ctttacaatt
    14461 cacgcccgtt ttttctaaga ggagcgcaac gtggaaaggt ttgatgccat tattataggc
    14521 gctggtgcgg cgggtatgtt ctgttctgcg ctggcaggtc aggcaggacg ccgggttctg
    14581 ctgatcgata atggtaaaat actagggcgc aaaatcctta tgtctggcgg tgggcgctgc
    14641 aactttacca acctttatgt cgaaccaggc gcttatctga gccagaatcc gcatttttgt
    14701 aagtctgcac tcgcacgttt tacccagtgg gatttcattg atctggtcaa taaacacggt
    14761 atcgcctggc acgagaaaac gttagggcaa ctcttctgcg atgactccgc gcagcagatt
    14821 gtcgacatgc tggtggatga gtgcgagaag ggcaatgtaa ccttcagatt gcgtagcgaa
    14881 gtgctgagtg tggcgaagga tgaaacaggc ttcacgcttg atctgaacgg catgactgtc
    14941 ggttgcgaaa agctggtcat cgcgaccggt gggctgtcaa tgccggggct gggcgcatcg
    15001 ccgtttggtt ataagattgc cgaacaattt ggcctcaacg tgctgccgac ccgcgcgggt
    15061 ctggtgccat tcactctgca taaaccgttg ctcgaagagt tacaggtgct ggcgggcgtg
    15121 gcggtgcctt ccgtgattac cgctgaaaac ggcaccgttt tccgtgagaa cttactcttc
    15181 acccaccgcg gcttatctgg accggcggtg ttgcagattt caagctactg gcaaccgggt
    15241 gaatttgtca gtatcaatct gctaccggat gtggacctcg aaaccttcct gaatgagcag
    15301 cgtaacgcac atccgaatca aagcctgaaa aacacactgg cggttcatct accgaagcgg
    15361 ttggttgaac gcttacagca actcgggcaa atcccggatg tttcgctaaa acagctcaac
    15421 gtgtgtgacc aacaggcact gattagcaca ttgaccgact ggcgcgtaca acccaacggc
    15481 actgaaggct atcgcactgc cgaagtgacg ctcggcggcg tggacaccaa cgaactttct
    15541 tcacggacga tggaagcgcg caaagtgcct gggctgtact tcatcggcga agtgatggac
    15601 gttaccggct ggctgggggg ctataacttc cagtgggcgt ggtcgagtgc atgggcttgt
    15661 gcgcaggatt tggcgtcaat tgtgaaataa ccatgacgtt ttcccgtcag ccgtttgtat
    15721 atgtcgttat taagtcattt tggtacggtt ctgcatccga cattctggtg ttaactcgct
    15781 gttttgttag cgaattaacg ctattgccgt tgtggttatt ttatagtgta tttattctca
    15841 gagaatatat attactgaaa taaaaaacac aaagaataac caaaggaaat tatttgaaac
    15901 gtaaaaggta tatattttta atctgtaaaa tttaaaatca ccccctctgg attgaatcaa
    15961 cttgctctat ttaagacgat tgtttttaca ttttcaccac ctgtatcagg tggtgttttt
    16021 ttattggcgg caatgccttt atcttatttt tattacgttg taaatatggc tattcctaaa
    16081 tgaaatttga tttgctcagt ttatttgtga tgtcagggga gtgcttgcca tgagagtcgt
    16141 gatattatta ttcatcattt ttataccgag tgtggcggat gctttttcat tcgatagttc
    16201 aataattaat ctcaaatgtc ctaggcgtgg aactatcgaa ataatattac acagatacga
    16261 acacacccag gaaagctggg gaggggagaa ttttgaaacc gggagtgggt atagtcatga
    16321 gggcgcgtta tttatttttg actttgccaa tcaggataaa atgatttaca atcgcgcgga
    16381 gaattcgttt gctttttggt atgaatcttc ccggaaatta gttaactgta acttaataac
    16441 gcttaaaagc acatatcctg tcaaccttcc ttatgttcat ggctgatgtg atgtgatgtg
    16501 atgtgtgtcg ggaactaaca tcttcatctt tgtatatgac attgcgataa tgttaagctg
    16561 gtctttgtca taaatttacc cttagagata ctactccctt ctgtcaatat tattcaagcc
    16621 gaacgttata ctggcttttg tcggtcacgg atatcttttt ttcagtgaga attcaaagtg
    16681 agggtatata agaagtcgta tgagtaagta tctttgttgt ggtgggttat tattttatgg
    16741 gcaaaatatt aagcagcgaa atcttttttt tgcccagacg ttttttgtag aaaaaagaat
    16801 ttattagtcc ggctgaaaag caagaactgc tataactaat taagcgtaac ggaacggtac
    16861 aaaacatgcc cttacaacaa ttttcccgat tagcgctcac tct
//
LOCUS       DAETPZ010000083        16760 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.083, whole genome shotgun sequence.
ACCESSION   DAETPZ010000083 DAETPZ010000000
VERSION     DAETPZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.083"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            193..2047
                     /gene="ldtD"
                     /locus_tag="K0W59_003081"
                     /pseudo
     CDS             193..2047
                     /gene="ldtD"
                     /locus_tag="K0W59_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            2228..2776
                     /locus_tag="K0W59_003082"
     CDS             2228..2776
                     /locus_tag="K0W59_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HBI3024022.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2803..3450
                     /gene="gloC"
                     /locus_tag="K0W59_003083"
     CDS             2803..3450
                     /gene="gloC"
                     /locus_tag="K0W59_003083"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HBI3024023.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QSLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3673..4863)
                     /gene="aspC"
                     /locus_tag="K0W59_003084"
     CDS             complement(3673..4863)
                     /gene="aspC"
                     /locus_tag="K0W59_003084"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HBI3024024.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFSAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5048..6136)
                     /gene="ompF"
                     /locus_tag="K0W59_003085"
     CDS             complement(5048..6136)
                     /gene="ompF"
                     /locus_tag="K0W59_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HBI3024025.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6738..8138)
                     /gene="asnS"
                     /locus_tag="K0W59_003086"
     CDS             complement(6738..8138)
                     /gene="asnS"
                     /locus_tag="K0W59_003086"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBI3024026.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8307..9509)
                     /gene="pncB"
                     /locus_tag="K0W59_003087"
     CDS             complement(8307..9509)
                     /gene="pncB"
                     /locus_tag="K0W59_003087"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBI3024027.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9775..12387
                     /gene="pepN"
                     /locus_tag="K0W59_003088"
     CDS             9775..12387
                     /gene="pepN"
                     /locus_tag="K0W59_003088"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HBI3024028.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12594..13361)
                     /gene="ssuB"
                     /locus_tag="K0W59_003089"
                     /pseudo
     CDS             complement(12594..13361)
                     /gene="ssuB"
                     /locus_tag="K0W59_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
     gene            complement(13358..14005)
                     /locus_tag="K0W59_003090"
                     /pseudo
     CDS             complement(13358..14005)
                     /locus_tag="K0W59_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(14017..15162)
                     /gene="ssuD"
                     /locus_tag="K0W59_003091"
     CDS             complement(14017..15162)
                     /gene="ssuD"
                     /locus_tag="K0W59_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HBI3024029.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSEHYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(15159..16118)
                     /gene="ssuA"
                     /locus_tag="K0W59_003092"
     CDS             complement(15159..16118)
                     /gene="ssuA"
                     /locus_tag="K0W59_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HBI3024030.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPAGARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16111..16686)
                     /gene="ssuE"
                     /locus_tag="K0W59_003093"
     CDS             complement(16111..16686)
                     /gene="ssuE"
                     /locus_tag="K0W59_003093"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HBI3024031.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQPH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
BASE COUNT         4187 a         4320 c         4289 g         3964 t
ORIGIN      
        1 cataacggcg cgcttttctt taaacttctt tacattaggt tatgtaaaaa cgtatcggcg
       61 tttatatact gaagataagc ctgatgagta acaggcttgc tcgtcatact ttcgtgagta
      121 ttggcgttgt acaggcaagt cgtaaaataa cagcctggct attcagagta tgataaaaac
      181 agggggcaag ggatgttgct taatatgatg tgtggtcgtc ggctgtcggc aatcagtttg
      241 tgcctggccg taacattcgc tccactgttc aatgcgcagg ccgatgagcc tgaagtaatc
      301 cctggcgaca gcccggtggc tgtcagtgaa cagggcgagg cactgccgca ggcgcaagcc
      361 acggcaataa tggcggggat ccagccattg cctgaaggtg cggcagaaaa agcccgcacg
      421 caaatcgaat ctcaattacc cgcaggttac aagccggttt atcttaacca gcttcaactg
      481 ttgtatgccg cacgcgatat gcaacccatg tgggaaaacc gtgatgctgt taaagccttc
      541 cagcaacagc tggcagaggt ggcgattgct ggtttccagc cgcagtttaa taaatgggta
      601 gagttactga ccgatcctgg tgttaacggg atggcacgtg acgtggtgct ctctgatgcg
      661 atgatgggct atctccattt cattgcaaat attccgatca aaggcactcg ctggctatat
      721 agcagtaaac cttatgcgct ttcaacgccg ccgctttcgg tgattaacca atggcagcta
      781 gcgctggata aaggtcaatt gcctacgttt gttgcaggac tggcaccgca gcatccgcaa
      841 tatgcggcga tgcatgaatc gttactggcc ttactcagtg acaccaaacc gtggccccaa
      901 ctgaccggca aagcaacgtt gcgcccaggg cagtggagta acgacgtacc ggcgttgcgc
      961 gaaatattgc aacgcacagg catgttggac ggggggccga aaattactct acctggcgat
     1021 gacacgccaa ctgacgcggt agtcagccca tccgctgtta ctgttgaaac agcagaaact
     1081 aagccgatgg acaagcaaac gatgtctcgt agtaaacctg cgcctgccgt tcgtgccgcc
     1141 tacgataatg aactggtgga agccgttaaa cgttttcagg catggcaagg attgggagca
     1201 gatggtgcta ttggcccggc aacgcgtgac tggttaaacg taacgcccgc ccagcgtgct
     1261 ggcgtgttgg cactcaacat ccagcgattg cgcttgctgc caacagagct ttctaccggg
     1321 atcatggtta acattccagc ctattcgctg ctctactatc agaacggcaa tcaggtgctg
     1381 gattcgcgag tcattgtcgg tcgccccgat cgcaaaacgc cgatgatgag cagtgcactt
     1441 aacaacgtag tggtaaaccc gccgtggaac gtaccaccaa ctctggcacg caaagatatt
     1501 ctgccgaaag tgcacaacga tccgggatat ctcgaaagcc atggctatac ggtgatgcgc
     1561 ggctggaaca gcagagaagc gattgaccca tggcaggttg actggtctac aatcacggcc
     1621 tcgaatttac cgtttcgctt ccagcaggct ccaggcccac ggaactcgct ggggcgctat
     1681 aaattcaata tgccgagttc agaggccatt tatttgcatg acacgccgaa ccacaatctg
     1741 ttcaagcgtg atacacgcgc attgagctca ggctgtgtac gagtgaataa agcttccgat
     1801 ctggcgaata tgctgttgca ggatgcagga tgcaggatgg aatgacaaac gtatttctga
     1861 tgctctgaag cagggtgata cacgttacgt caatattcgg cagtcgattc cggtgaatct
     1921 ctactacctg acggcctttg ttggtgcaga tgatcgtacc cagtatcgta cagatattta
     1981 caattatgat ctgcctgcgc gatccagctc gcaaatcgta tcgaaagcgg aacaattaat
     2041 caggtaaatg aagtagttcg gggaaatcaa ttgtcgtaat aattagcatg attgggggcg
     2101 attctctgca gcccccgtca ctgctggggt tgagtcatct tgacgtctgc tttacgggcg
     2161 gttaaggtgc ctcttgtgcg ccagaagtgc atataaacga taacattgac ctgtagactt
     2221 gattatcatg gacaaattcg acgctaatcg ccgcaaattg ctggcgcttg gtggcgttgc
     2281 actcggtgcc gccatcctgc cgacccctgc gtttgcaaca ctctctaccc cacgcccgcg
     2341 cattttgaca ctcaataatc ttcataccgg agagtcaatc aaagcggagt ttttcgatgg
     2401 cagaggctat attcaggaag aattggcaaa acttaaccat tttttccgcg attaccgcgc
     2461 gaacaaaata aagtccatcg acccaggatt attcgaccag ttgtatcgcc tgcaagggtt
     2521 gttaggcacg cgcaaaccgg tgcaactcat ttccggttat cgttccattg ataccaacaa
     2581 tgaactacgc gcccgcagcc gtggagtagc gaagaaaagc tatcacacta aaggccaggc
     2641 gatggatttc catattgaag gtatcgcgtt aagcaatatt cgcaaagccg cgttatctat
     2701 gcgcgcaggt ggtgtaggat attacccacg tagtaacttt gtgcatattg ataccgggcc
     2761 agcacggcac tggtagtaat cgcttaacga aacaggggca gtatgaacta tcgtattatt
     2821 ccggtcaccg cattctccca gaactgttca ttgatctggt gtgaacaaac ccgcctggcc
     2881 gcactggtcg atcctggcgg cgatgcggaa aaaatcaaac aggaagttga tgacagcggc
     2941 ctgacactga tgcagatcct gctgacgcat ggtcatctgg accacgttgg cgcagcggcg
     3001 gaactggcgc aacattacgg cgtgccggtt tttggcccgg aaaaagaaga tgagttctgg
     3061 ctgcaaggct tgcctgcgca aagtcgtatg tttggtctgg aagagtgcca gtcgctgacg
     3121 ccagatcgtt ggctgaacga aggcgatacc atcagcatag ggaatgtgac tttacaggtg
     3181 ttacattgcc ctgggcatac gccgggtcat gtcgtgtttt ttgatgatcg ggcaaagctg
     3241 ctgatttctg gcgatgttat tttcaaaggc ggagtagggc gcagtgactt cccgcgtggc
     3301 gatcataatc aactgatttc ttcaatcaaa gataaattgc tgcctctggg ggatgacgtg
     3361 acatttattc cgggtcacgg accattatct acacttggtt atgagcgcct gcacaatccc
     3421 ttcctgcaag acgaaatgcc cgtctggtaa aggcgcataa aaaagtccac ttttaatgcg
     3481 ggcctggatt tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc
     3541 cggcctacaa aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt
     3601 agcgcatcag gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat
     3661 tgtttttaac gcttacagca ctgccacaat cgattcgcac agcggagcca tgttatctgg
     3721 tgtcatcccg gccacattta cgcgaccaga agcaacagca tatacgccaa actcttcgcg
     3781 cagacgcagc acttgttctt ttgtcagacc actgaaggag aacatgccgt tctgtttgat
     3841 gataaagctg aagtcgcggt ttgcaccttt ttcctgcagc gtattgacga acaactgacg
     3901 catacgctga atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaatgc
     3961 atcgttgctc aggatggtgg caacaacaga agcgccgtgt gctggagggt tagagtagtt
     4021 agcgcgaatc gccgctttca tttggctgaa tgcgcgatca acgatttcac tgtcggcagc
     4081 aaccagagta caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact
     4141 ggcaacaatc agctctttat gcatagccga gaaagcgcgc agtccttcag catcttcttc
     4201 cagaccacgg gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac
     4261 ggagagttgt gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg
     4321 gcagcagcca tggaacagca ctacgtcgcc agcctgagct tcgttcagac tgttaatcag
     4381 tgcatcgaag tcaagggtgt gattttccgc atcataataa gcgtattcac gaacttccag
     4441 acctgcagag ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacacacg
     4501 cttaacgctg gtattttttg ccaggaagtc ggcagccacg cgtagtgcgc cagtgccacc
     4561 cggagtctgt gccgtgcgag cacgtttgtc attgatcagg gcgctacctt taccaaacag
     4621 cagttcctga gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt
     4681 ggtttcattt tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt
     4741 gcccgtctca tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc
     4801 acgaaacaga tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa tgttctcaaa
     4861 catgacgagg ttccattatg gttatagaag ggaagtccgc tatcagggta acgggagatt
     4921 tacaaaattc caattattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc
     4981 tctggaaggc ataaaaaaaa caggaccaac gtcctgtttt ttcggcattt aacaaagagg
     5041 tgtgctatta gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca
     5101 gtttgttgtc agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt
     5161 tgaagtagta ggttgcgccc acttcaaagt agttcaccag atcaacatca ccgatacctt
     5221 ctacgtcttt cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact
     5281 ggtattgcgc aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa
     5341 atttattagt gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt
     5401 tgttcgcgtc gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag
     5461 gttgagcttc ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc
     5521 cttcgtattc gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt
     5581 cacgctcgtt tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga
     5641 agttggagtt acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt
     5701 atgcagtatc accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca
     5761 caccgtagtt acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg
     5821 ccagacgcgt tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt
     5881 tatattccca ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac
     5941 caagacgggc ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg
     6001 aaaaataatg cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat
     6061 agatttctgc agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa
     6121 tattgcgctt catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcc
     6181 gtcaataagt tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat
     6241 gtgtctgcag gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag
     6301 ataaagattt agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt
     6361 gtgaacaccg tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt
     6421 atatgattta cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta
     6481 tcgacctggc acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt
     6541 gtgctatcag ctaagaaaaa acgaatccgc aataagaatg cgggttattt tttgtgcgta
     6601 aatgttgcaa tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt
     6661 gcttgactta acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg
     6721 ctctttgagg aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca
     6781 tcacgcacgt tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa
     6841 cctgaatgcg gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta
     6901 ttcaggccca tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag
     6961 ccaccaatga tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg
     7021 tcttcgttga ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc
     7081 ggtgctttaa agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc
     7141 cagtaaaccg ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg
     7201 taatccacct gtgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta
     7261 tctacgcgtt cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag
     7321 acatatttca gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact
     7381 tccggctcca gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg
     7441 aaagtcgggc cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac
     7501 tggccagata cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg
     7561 ccctgatcgt tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct
     7621 gcaccttcgg tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg
     7681 ttaaagaagc gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc
     7741 aggtttgtgc gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt
     7801 gccgccatcg ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc
     7861 tgaatttcaa attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc
     7921 gagcagccgg tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg
     7981 acagcctgta caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct
     8041 tttgaatctc ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg
     8101 cggccctgga gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc
     8161 ggaaaaaata aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct
     8221 gcaatcagac aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa
     8281 gggagccgat tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac
     8341 gctttgcgca gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca
     8401 gaaagtttcg ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt
     8461 tttacctggg ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg
     8521 gaagagaagt ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc
     8581 agcgttttac tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc
     8641 cattcaaccg ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca
     8701 ccgaaatcac gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg
     8761 tcgggatact cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg
     8821 ctgatttgct gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa
     8881 agccgacgcg ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc
     8941 ttaacgatgg tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga
     9001 tggaagcgcg acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc
     9061 agcgtgtcga gggcttgtgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca
     9121 ctgataaccg ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag
     9181 cgaatatcca gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac
     9241 tcgcgtaacc agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat
     9301 tcatcatcct gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca
     9361 taaataccca gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa
     9421 tagtgatgaa acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag
     9481 tgcagaacag gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg
     9541 tttagtacaa taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga
     9601 accccgatca caccatatgc cacctttctg gacgatggca ttacgcggtg tatgttataa
     9661 aaatgtagca ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta
     9721 cgcaacagga atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact
     9781 caacagccac aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat
     9841 attgacttga cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct
     9901 gtccgtcatg gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt
     9961 tctgttcata ttaatgatga gccgtggacc gcctggaaag aagaagaggg cgcactggtc
    10021 atcagtaatt tgccggagcg ttttacgctt aagatcatta atgaaataag cccggcggcg
    10081 aatacggcgc tggaagggct ttatcagtca ggcgatgcgc tttgcaccca gtgtgaagcc
    10141 gaaggtttcc gccatattac gtattatctc gaccgcccgg acgtgctggc gcgttttacc
    10201 accaaaatta ttgccgataa aatcaaatat cccttcctgc tttccaatgg taaccgcgtt
    10261 gcgcaaggcg aactcgaaaa cggacgccat tgggtacagt ggcaggaccc gttcccgaaa
    10321 ccgtgctacc tgtttgcgct ggtggcaggc gactttgatg tactgcgcga tacctttacc
    10381 acgcgttctg gtcgcgaagt agcactggag ctgtacgtcg atcgcggcaa ccttgatcgc
    10441 gcgccgtggg cgatgacctc gctgaaaaac tccatgaaat gggatgaaga acgctttggc
    10501 ctggagtatg acctcgacat ctatatgatc gtcgcggtgg atttcttcaa tatgggcgca
    10561 atggagaata agggtctgaa tatctttaac tccaaatatg tgctggcccg caccgacacc
    10621 gccaccgaca aagattacct cgatattgaa cgcgttatcg gccatgaata tttccataac
    10681 tggaccggta accgagtgac ctgccgcgac tggttccagc tcagcctgaa agaaggttta
    10741 accgtcttcc gcgatcagga attcagctct gaccttggtt cccgcgcagt taaccgcatc
    10801 aataatgtgc gcaccatgcg cggattgcag tttgcggaag acgccagccc gatggcgcac
    10861 ccaatccgcc cggacatggt cattgagatg aacaacttct acaccctgac cgtttacgag
    10921 aagggcgcgg aagtgatccg tatgatccac accctgctgg gcgaagaaaa cttccagaaa
    10981 gggatgcaac tctatttcga gcgtcatgat ggtagcgcag cgacctgtga cgactttgtg
    11041 caggcgatgg aagatgcgtc gaatgttgat ctctcccatt tccgccgttg gtacagccag
    11101 tccggtacgc cggttgtgac cgtcaaagac gactacaatc cggaaactga gcagtacacc
    11161 ctgaccatca gccagcgcac gccagccacg ccggatcagg cagaaaaaca gccgctgcat
    11221 attccgtttg ccatcgaatt gtatgacaac gaaggcaaag tgatcccgtt gcagaaaggc
    11281 ggtcatccgg tgaattccgt gctgaacgtc acccaggcgg aacagacctt tgtttttgat
    11341 aatgtctact tccagccggt gcctgcgctg ctgtgcgaat tctctgcgcc agtgaaactg
    11401 gaatataagt ggagcgatca gcaactgacc ttcctgatgc gtcatgcgcg taatgatttc
    11461 tcccgttggg atgcggcgca aagtttgctg gcaacctaca tcaagctgaa cgtcgcccgt
    11521 catcagcaag ggcagccgct gtctctgccg gtacatgtgg ctgacgcttt ccgcgcggtg
    11581 ctgctcgatg agaagattga tccggcgctg gcggcagaaa tcctgacgct gccttctgtc
    11641 aatgaaatgg ctgaactgtt cgatatcata gacccgattg ctattgccga agtacgcgaa
    11701 gcactcactc gtactctggc gactgaactg gcggatgagc tgctggctat ttacaacgcg
    11761 aattaccaga gcgagtaccg tgttgagcat gaagatattg caaaacgcac tctgcgtaat
    11821 gcctgcctgc gcttccttgc ttttggtgaa acgcatctgg ctgacgtgct ggtgagcaag
    11881 caataccatg aagcaaacaa tatgaccgat gcgctggcag cgctttctgc ggcggttgct
    11941 gcacagctgc cttgccgtga cgcgctgatg caggagtacg acgataagtg gcatcaggac
    12001 ggtctggtga tggataaatg gtttatcctg caagccacca gcccggcggc gaatgtgctg
    12061 gagacggtgc gtggtctgtt gcagcatcgc tcatttacca tgagcaaccc gaaccgtatt
    12121 cgttcgttga ttggtgcgtt tgcgggcagc aacccggcag cgttccatgc cgaagatggc
    12181 agcggttacc agttcctggt ggaaatgctt accgacctca acagccgtaa cccgcaggta
    12241 gcttcacgtc tgattgaacc gctgattcgc ctgaaacgtt atgatgccaa acgtcaggag
    12301 aaaatgcgcg cggcgctgga acagttgaaa gggctggaaa atctctctgg cgatctttac
    12361 gagaagataa ctaaagcact ggcttgatac ttttgagcca aaatgcctga tgcgacgctt
    12421 gacgcgtctt atcaggccta caactccgtt gaaattgctg gttttgtagg ctggataagg
    12481 cgttcacgcc gcatccggca gccgtgccca actgcctgat gcgatgcttg acgtgtctta
    12541 tcaggcctac aagtccgttg aaaatgctgg ttttgtgcgc cacatccggc atattaacca
    12601 tgacgccgaa tcagttgttc agaatgccca cgccgcatta cccgttgcaa tacttccgct
    12661 tccagctccg ctaatcgcac cgaccctaat cgacgcggac gggggatatc cactgtcaaa
    12721 tccagactaa tttttccctc ttcaattaac aacacccggt cagccattgc taccgcttcg
    12781 ctcacatcat gcgtcaccaa cagtacggta aagccgtgct gctgccaaag tgacacaatc
    12841 aaatcctgca tctcgagtcg cgttaaggcg tccagtgccc cgagcggttc atcaaacaac
    12901 aataaacccg gtcgatgaat caacgctctt gccagcgcca cacgctgttt ctgcccgcca
    12961 gaaagtgcgg caggccattc cccggcgcga ttctccagtc ctaccgcagc caacgcccga
    13021 cgcgcggcat cgcgccactg acctttaagg cctaacccaa cgttatcaat caccgatttc
    13081 tacggcagca gacgcgcatc ctgaaacatc attcgcgtat catcctgaat ttcagccagc
    13141 ggtgtggtgc ccgctaacac atcgcctacg gttggcgttt ccagaccggc cagcaggcgc
    13201 agcagggtac ttttaccacc accgctgcgg cccaccaccg ccacaaactg acctgccgga
    13261 atatgtaaat ccagttggtt caggacgata ttttccgcgt aatgtttgct tactgcattg
    13321 agcaacaatg gtgtgccctg gttcagacga gcagtattca tatcgtggcc tccttcaaat
    13381 gataagccgg gttccagctc aaccagaggc gctctaacag ctgcgcgctg acgtcagcaa
    13441 gtttgccgag cagggcgtaa agaataatag cgactaccac cacgtctgtt tgcaaaaact
    13501 ctcgcgcatt catcgccaga taaccaatgc cggaattggc agaaatggtt tcggcaacaa
    13561 tcagcgtcag ccacatcagg cccaacgcaa aacgcacgcc gaccataatt gagggcaggg
    13621 catcgggcag gatcacatgg ataaacagcg gtatgccgga taatccatag ctacgcgcca
    13681 tctccaccag cccgcgatcg atattacgga tgccatgcca ggtgttgata taaatgggga
    13741 acagcgtacc gagcgccacc agaaagattt tcgcggattc atcaatgcca aaccacaaaa
    13801 tcaccagcgg gatcagcgcc agatgctgcc acagttcgcc gctggcggag agcgtccaga
    13861 acgccgtcac cactccttcc ggtgaaggca aaatacgcgt cgacagccag ccaaccgagg
    13921 aggccagttg ccacaccgcc acgatgccca ccggtaaaaa ccagggggca acgcgcaata
    13981 actacttctt cactggcgtt gccatcatcg tgttccttag ctttgcgcga ctttacgggg
    14041 gataaaatca ttcgccaccg cttcgccttg cggattcagc ggctgcggct ggggaatttc
    14101 cggaatggcg acatccagat gtgggaacag caactcgcca acccgatacg cttcttccag
    14161 atgcgggtag cccgaaagca caaagctgtc gatgccaagc gcggcgtatt cgttgattcg
    14221 cgcggcgacc gtaggaccat cgcccaccag cgccgttccg gcaccgccgc gcactaagcc
    14281 aacgcccgcc cataaattgg ggctgatctc cagattgtcg cgcttgccgt tatgtagcgc
    14341 cgccattcgc tgttgtccta cggaatccgt ccgggcgaat gcggcttgtg ctttggcgat
    14401 agtctcatca tcaagatgcg agattaaccg ctcggcggcc tgccacgctt cgtcgttagt
    14461 ttcacgaaca atcacatgca gacgaatacc gaaacgaatt ttgcgcccat acgcggcagc
    14521 tttcgcccgc acttgttcga ttttctcttt aacaagttcc ggcggttcgc cccaggtgag
    14581 gtagagatca acttgttctg ccgccagctc ctgggcgaca tctgacgatc cgccaaagta
    14641 aagcggagga taaggctgtt gaatcggcgg gaagagcagt tttgctccgc gcacatgaat
    14701 atgtttaccg ttgaaattga cggtttcccc cagcaataag cgccgccaga cctgggtaaa
    14761 ttccgccgag gcttcgtagt gctcgctatg atcaaggaac acgccgtcgc ctgccagctc
    14821 ttgtggatcg ctgcctgtga ccagattaaa caacgcacga ccatttgaga gacggtcaag
    14881 cgtggcggcc tggcgggcgg caacagtagg tgaggttacg ctgggacgca gggcgacaag
    14941 aaacttcagc cgctgcgtca ccgggatcat cgacgctgca accagccacg catcttcaca
    15001 cgatcgcccc gttgggatca gcacaccggt atagcccaga cgatccgccg cttgcgcaat
    15061 ttgttgcaga taaccgtgat caaccgggcg tgaaccttct tccgttccca gatagtgccc
    15121 gtcaccgtgg gtcggtaaaa accagaacat attcagactc ataattgttt tccttccagt
    15181 tgagtgggct gccagatgcg ctggcgaata tcgacttttt tcggcaccag acggttttca
    15241 taaaacagat ctgccgtttg ctgctgtaag gcggcaacct cggcgttaac cggtttgatg
    15301 gtggtaggag ggcgatgatc taagtaagag gcaatcaccg gtgccggtaa gcccatcgtt
    15361 tttgccagta aagcgatgct ttgttcgcgc tggctgcggg ttaacgcatc ggcctcacta
    15421 aaggttgcca gtacgccctg aataaaagcg ccgttttttt ctgcataggg acgagctgcc
    15481 agataaaacg atccagtttg attgagatcg gtgccgtctt tcagcacccg cacgccgccc
    15541 tgtaataacg cagcggagta gtagggatcc cagatagccc aggcgtcaac gttaccttgc
    15601 tggaacgcgg cgcgggcacc ggcaggcgtc aggtaagtgg gctggatatc ggtgaattta
    15661 agtccggcct gacgcagtgc acgcagtaaa aggttgtgtg aactggaacc tttctgaaag
    15721 gcaactttgt gacctttaag atcggctacg gttttgatcg ggctgttttc tgccaccaga
    15781 atcacttcgg ctttgggctt cggtggctcg acgcccacat ataccaaatc agccccggca
    15841 gcctgggcaa agattggcgg aatatccccg gtactgccga gatcaatact gccaacgttt
    15901 aacgcttcca acatttgcgg acccgcgggg aactccaccc aggagatttt tgattgcgga
    15961 tagcgttttt ccagtaactg gtggcttttt gccagcacca taccaatact gcctttctga
    16021 tagcctatac gtaacgcttc aggcgaggat tctgcagcaa ccgcaaaagt agagacgcta
    16081 agcaatcccg ccagcgccag tttaatgatg ttacgcatgg gcattacctc gcagagacag
    16141 aaggtcagga acctgaacat cgcggcggtg caatgcctgc cagaaagttt ctagcgcggt
    16201 atcaagatgg ggttgcagat ttggcgtgaa ctggggtctg tgatggtaat caattaattg
    16261 tgagtcatcg gcaaacacgc cgtgcaggat ctcctgagct ttcagtgcgc ttaaaactgg
    16321 tttaagggca taatcgaccg ccagcagatg ggccacggta ccgcccgtcg ccagcggtag
    16381 caccactttg ccttgcaaag cgcgctctgg cagcaggtcg agcagggttt tcagcgcgcc
    16441 ggaataggcg gctttataca caggcgtggc gacaatcagc ccatcagcct gttgcagctg
    16501 ttcggtgaag gtcttgagtg ccggactatc gaaacgagca taaagcagat cttccggggc
    16561 gaagttttgc agattccagt gataaacctc tacatctagg ccatttagtt tttcccgcgc
    16621 atattccagc aaggagctgg agcgagaagg aaagcgagga ctacccgcca gggtgatgac
    16681 acgcatactc tctccttata accaattgtt cttttttttg ttaacattga taacaattcg
    16741 gtcagtctgt cggagagaca
//
LOCUS       DAETPZ010000084        16327 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.084, whole genome shotgun sequence.
ACCESSION   DAETPZ010000084 DAETPZ010000000
VERSION     DAETPZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.084"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(90..2066)
                     /gene="speA"
                     /locus_tag="K0W59_003094"
     CDS             complement(90..2066)
                     /gene="speA"
                     /locus_tag="K0W59_003094"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HBI3024032.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENALPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2075..2206)
                     /gene="yqgB"
                     /locus_tag="K0W59_003095"
     CDS             complement(2075..2206)
                     /gene="yqgB"
                     /locus_tag="K0W59_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HBI3024033.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2342..2557
                     /locus_tag="K0W59_003096"
     CDS             2342..2557
                     /locus_tag="K0W59_003096"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024034.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2861..4015
                     /gene="metK"
                     /locus_tag="K0W59_003097"
     CDS             2861..4015
                     /gene="metK"
                     /locus_tag="K0W59_003097"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBI3024035.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4452..5846
                     /gene="galP"
                     /locus_tag="K0W59_003098"
     CDS             4452..5846
                     /gene="galP"
                     /locus_tag="K0W59_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HBI3024036.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5923..6420
                     /locus_tag="K0W59_003099"
     CDS             5923..6420
                     /locus_tag="K0W59_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HBI3024037.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6515..7222
                     /gene="endA"
                     /locus_tag="K0W59_003100"
     CDS             6515..7222
                     /gene="endA"
                     /locus_tag="K0W59_003100"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HBI3024038.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7302..8033
                     /gene="rsmE"
                     /locus_tag="K0W59_003101"
     CDS             7302..8033
                     /gene="rsmE"
                     /locus_tag="K0W59_003101"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBI3024039.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8046..8996
                     /gene="gshB"
                     /locus_tag="K0W59_003102"
     CDS             8046..8996
                     /gene="gshB"
                     /locus_tag="K0W59_003102"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HBI3024040.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9105..9668
                     /locus_tag="K0W59_003103"
     CDS             9105..9668
                     /locus_tag="K0W59_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HBI3024041.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9668..10084
                     /gene="ruvX"
                     /locus_tag="K0W59_003104"
     CDS             9668..10084
                     /gene="ruvX"
                     /locus_tag="K0W59_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBI3024042.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10260..11240)
                     /locus_tag="K0W59_003105"
     CDS             complement(10260..11240)
                     /locus_tag="K0W59_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBI3024043.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHIIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11258..11962
                     /gene="yggS"
                     /locus_tag="K0W59_003106"
     CDS             11258..11962
                     /gene="yggS"
                     /locus_tag="K0W59_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HBI3024044.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11980..12546
                     /gene="yggT"
                     /locus_tag="K0W59_003107"
     CDS             11980..12546
                     /gene="yggT"
                     /locus_tag="K0W59_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HBI3024045.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            12543..12833
                     /locus_tag="K0W59_003108"
     CDS             12543..12833
                     /locus_tag="K0W59_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HBI3024046.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12841..13434
                     /locus_tag="K0W59_003109"
     CDS             12841..13434
                     /locus_tag="K0W59_003109"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HBI3024047.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAEMTREEKSAISHRGQALKLLLDALRNG"
     gene            13427..14563
                     /gene="hemW"
                     /locus_tag="K0W59_003110"
     CDS             13427..14563
                     /gene="hemW"
                     /locus_tag="K0W59_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HBI3024048.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRSRLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGMQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14877..15840
                     /locus_tag="K0W59_003111"
                     /pseudo
     CDS             14877..15840
                     /locus_tag="K0W59_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15885..>16327
                     /locus_tag="K0W59_003112"
     CDS             15885..>16327
                     /locus_tag="K0W59_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBI3024049.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYA"
BASE COUNT         4043 a         4204 c         4110 g         3970 t
ORIGIN      
        1 aaaagcagct agattatttt taagaatata taaaaagaaa gcgggccgat ttacgtaaat
       61 aaatcgaccc gccagcggaa taatcttaat tattcatctt caagataagt ataaccgtac
      121 aaacctgcct cgaactcttc aaggaactgt tgttgcagtt cagcatcaag atcggttttc
      181 ttcacttgat cgcggaactg ggttaacagc gttttcggat cgagctgtac atattgcagc
      241 atgtccgcca cggtatcgcc ttcgtcagac agttctactt ctacgctacc gtcagggaac
      301 acgaacacgt caactgcttc ggtatcaccg aacaggttgt gcatattgcc aaggatctcc
      361 tgatatgcac cgaccataaa gaaaccgagc atcggcggat tctctggatc gtactccggc
      421 attggcattg tcgtggcaat accatcacca tcaatatagt ggtcgatagc accgtcagag
      481 tcacaggtaa tatccagcag cacagcgcga cgttccggca cttgatccag cccttccagc
      541 ggtagaaccg ggaacaactg gtcgatcccc catgcgtccg gcattgactg gaacagcgag
      601 aaattgacgt acattttgtc cgccatacgt tcctgcagtt cgtcgattat cggacgatgc
      661 gcacggtttt gcggatccag ttgcttctgc acttcatggc acatgctcag atagagttgt
      721 tctgcccatg cacgttcttg caggctaaag gtgccggaag agtagccgat atgaatgtcg
      781 tgcagatcca tctggctgtc gtgtaaccat tcacgcaaag aacggcgagt tcccggttcg
      841 tgcatttcct gccaggtttc ccacatgctt tgcagcgcac gcggcgcatc ttctgcgggc
      901 gcggtcggca ccgtatattc gttacgttcc acgccgatga tattcgacac cagcacggta
      961 tggtgcgcag tcaccgcacg gcccgattcg gtgattaccg tcggatgcgg cagagcattt
     1021 tcttcacacg catcgccaat cgcccagatg atgttgttgg cgtattcatt gaggccatag
     1081 tttacggagc agtcagactg cgaacgggtg ccttcataat ccacgcccag accgccgccg
     1141 acgtcgaagc actgaatatt gacgcccagc ttgtgcagtt ctacatagaa acgcgcggat
     1201 tcacgaacgc ctgtcgcgat atcgcgaata ttcgccatct gcgaaccgag gtggaagtgc
     1261 agtagttgca ggctgtcgag acgcccggct tcacgcaggg tttcaaccag ttgcagtacc
     1321 tgagtcgcag ccaggccgaa cttcgatttt tccccgccgg aggactgcca tttacccgag
     1381 ccctgcgaag ccagacgtgc acgcacgccc agacgaggaa cgacattcag acgttctgct
     1441 tcatccagca caatggcgat ttctgacatc ttctcaatga ccagatagac cttgtgcccc
     1501 atcttctcgc caattaatgc caggcggata tattcgcggt ctttataacc gttgcagacg
     1561 atgacactac gagtcatgcc agcatgtgcc agcactgcca tcaactcggc tttggaaccg
     1621 gcttccagac ccagcggttc acccgaatga atcagggact cgatcacgcg gcggtgctgg
     1681 ttaactttga tcggataaac aaggaagtaa tcgccgttat agccgtagga ttccctcgca
     1741 cgtttgaacg cggcgttaat ggaacgcaaa cggtgctgca ggatctgtgg gaaacagaac
     1801 agtgcaggca gacgctgacc ctgtgcttca cgagttttca ctaactgcgc gagatcgacg
     1861 cgagcttccg ggacgtccgg gtccgggcac acgctgatgt ggcccagctc gttaacgtca
     1921 tagtagttat tgccccacca ggcaatattg taagtacgca gcatcttgct ggcttcctgg
     1981 gagctcattg caacctcctg catggagcgt agtacaccgt gttcgcccgc tgacgaaggc
     2041 aaacccatag acatgtcgtc agacatagcg aacctcaaat tattttatta agtgtaaaac
     2101 agttaacgac tgtcgcagcc tggaactgcg ataacaaccc ataagcacat ggattttcca
     2161 gcagtgtatg ctgacgctcc gactgcgcaa ccggtttctt tttcataaca ttattaagca
     2221 cataaccgaa cgtaagtgtg acagttcggc gaaaccacga gaaaactctt gtttttacaa
     2281 gagcgccctt gttcagtcct cagtaactgt aaccagctct tgaatcctga gaagcgccga
     2341 gatgggtata acatcggcag gtatgcaaag cagagatgca gagtgcgggg aacgaatctt
     2401 caccagaacg gtgagacagg ttaagcagca gacaacggtt cattatttcg tatcacctcc
     2461 acggccgcct gttaagacga acccacaagc caaaactctg atttcaaccc ggctggaagt
     2521 ggcaacacga aaaaaacgtc gtgtgctttt tatttaagcc gcgcgccgcg ttttataccc
     2581 cacaatggca gaaaattgca aaagataaat acgcagaatg ccggcattgt caggaaaaat
     2641 ttccagccac gtttttaaca gaatgagaca cgattcaaaa aaaagtggaa atcgggtgaa
     2701 gaattgacct aaaatagcca tccagatgtt aatccatcca taccgattaa cactcagact
     2761 gccagtgttt ttaacctgca gagtcgtggt aggatccgct accacagaaa atccacacaa
     2821 cagtttgagc taaccaaatt ctctttaggt gatattaaat atggcaaaac acctttttac
     2881 gtccgagtcc gtctctgaag ggcatcctga caaaattgct gaccaaatct ctgatgccgt
     2941 tttagacgcg atcctcgaac aggatccgaa agcacgcgtt gcttgcgaaa cctacgtaaa
     3001 aaccggcatg gttttagttg gcggcgaaat caccaccagc gcctgggtag acatcgaaga
     3061 gatcacccgt aacaccgttc gcgaaattgg ctatgtgcat tccgacatgg gctttgacgc
     3121 taactcctgt gcggttctga gcgctatcgg caaacagtct cctgacatca accagggcgt
     3181 tgaccgtgcc gatccgctgg aacagggcgc gggtgaccag ggtctgatgt ttggctacgc
     3241 aactaatgaa accgacgtgc tgatgccagc acctatcacc tatgcacacc gtctggtaca
     3301 gcgtcaggct gaagtgcgta aaaacggcac tctgccgtgg ctgcgcccgg acgcgaaaag
     3361 ccaggtgact ttccagtatg acgacggcaa aatcgttggt attgatgctg tcgtgctttc
     3421 cactcagcac tctgaagaga tcgaccagaa atcgctgcaa gaagcggtaa tggaagagat
     3481 catcaagcca attctgcccg ctgaatggct gacttctgcc accaaattct tcatcaaccc
     3541 gaccggtcgt ttcgttatcg gtggcccaat gggtgactgc ggtctgactg gtcgtaaaat
     3601 tatcgttgat acctacggcg gcatggcgcg tcacggtggc ggtgcattct ctggtaaaga
     3661 tccatcaaaa gtggaccgtt ccgcagccta cgcagcacgt tatgtcgcga aaaacatcgt
     3721 tgctgctggc ctggccgatc gttgtgaaat tcaggtttcc tacgcaatcg gcgtggctga
     3781 accgacctcc atcatggtag aaactttcgg tactgagaaa gtgccttctg aacaactgac
     3841 tctgctggta cgtgagttct tcgacctgcg cccatacggt ctgattcaga tgctggatct
     3901 gctgcacccg atctacaaag aaaccgcagc atacggtcac tttggtcgtg aacatttccc
     3961 gtgggaaaaa accgacaaag cgcagctgct gcgcgatgct gccggtctga agtaatcttt
     4021 cttcacctgc gttcaaaggc cagcctcgcg ctggcctttt tcttttacct caccgcggtg
     4081 gataggcgtt cacgccgcat ccagcaaaaa aactgcccgc acaataacat cattcttcct
     4141 gatcacgttt cacggcaaat taccatcaca attgaaaccg attacaccaa ccacaccagc
     4201 aaaatatttg taatattttc atattattat tcagctttca cagttgttac atttcttttc
     4261 agtaaagtct taattgcaga tgacagcgtt taatctatga tgatataact caattatttt
     4321 catgcactta aatcataact aagataaatg ttagtgtaag cgattacact gatgtgattt
     4381 gcttcacatc tttttacgtc gtactcacct atcttaattc acaataaaaa ataaccatat
     4441 tggagggcat catgcctgac gctaaaaaac aggggcggtc aaacaaggca atgacgtttt
     4501 tcgtctgctt ccttgccgct ctggcgggat tactctttgg cctggatatc ggtgtaattg
     4561 ctggcgcact gccgtttatt gcagatgaat tccagataac ttcgcacacg caagaatggg
     4621 tcgtaagctc catgatgttc ggtgcggcag tcggtgcggt gggcatcggc tggctctcct
     4681 ttaaactcgg gcgcaaaaag agcctgatga tcggcgcaat cctgtttgtt gccggttcac
     4741 tgttctctgc ggctgcgcca aacgttgaag tactgattct ttcgcgcgtt ctgctggggc
     4801 tggcggtggg tgtggcctct tataccgcac cactgtacct gtcagaaatt gcgccggaaa
     4861 aaattcgcgg cagtatgatc tcgatgtatc agttgatgat cactatcggg atcctcggtg
     4921 cttatctttc tgataccgcc ttcagctaca ccggtgcatg gcgctggatg ctgggtgtga
     4981 ttatcatccc ggcaattttg ctgctgattg gtgtcttctt cctgccagac agcccacgtt
     5041 ggtttgccgc caaacgccgt tttgttgatg ccgaacgcgt gctgctacgc ctgcgtgaca
     5101 ccagcgcgga agcgaaacgc gaactggatg aaatccgtga aagtttgcag gttaaacaga
     5161 gtggctgggc gctgtttaaa gagaatagca acttccgccg cgcggtgttc cttggcgtac
     5221 tgttacaggt aatgcagcaa ttcaccggga tgaacgtcat catgtattac gcgccgaaaa
     5281 tcttcgaact ggcgggttat accaacacca ccgagcaaat gtgggggaca gtgattgtcg
     5341 gcctgaccaa cgtacttgcc acctttatcg caatcggcct tgttgaccgc tggggacgta
     5401 aaccaacgct aacgctgggc ttcctggtga tggctgctgg catgggcgta ctcggtacaa
     5461 tgatgcatat cggtatccac tctccgtcgg cgcagtattt cgccatcgcc atgctgctga
     5521 tgtttattgt cggttttgcc atgagtgccg gtccgctgat ttgggtactg tgctccgaaa
     5581 ttcagccgct gaaaggccgc gattttggca tcacctgctc caccgccacc aactggattg
     5641 ccaacatgat cgttggcgca acgttcctga ccatgctcaa cacgctgggt aacgccaaca
     5701 ccttctgggt gtatgcggct ctgaacgtac tgtttatcct gctgacattg tggctggtac
     5761 cggaaaccaa acacgtttcg ctggaacata ttgaacgtaa tctgatgaaa ggtcgtaaac
     5821 tgcgcgaaat cggtgctcac gattaatctc ctcaggcttc ctcccgtcgc ggaggaagcc
     5881 gcctcttgca gtcatctttt cttcgctcta tcctctgccg ctatgaaaac atcccgtctc
     5941 cctatagcca tccaacaggc cgttatgcgc cgcctgcggg aaaaactcgc ccaggccaac
     6001 ctgaagctag ggcgtaacta cccggagcca aaactctctt acacccagcg cggaacctcc
     6061 gccggaacag cctggctgga aagctatgaa atccgcctca atcccgtttt gctgttggaa
     6121 aacagtgaag cttttattga agaagtggta ccgcacgaac tggcacattt gctggtatgg
     6181 aaacatttcg gccgcgtagc gccacatggc aaagagtgga agtggatgat ggaaagcgtg
     6241 ctgggcgttc ccgcccgtcg tacgcatcag ttcgaactgc aatccgtgcg tcgcaacacc
     6301 ttcccctacc gctgtaagtg ccaggaacat cagcttaccg tacgccgcca taatcgcgta
     6361 gttcgtggcg aggccatcta tcgctgtgtt cactgcggtg aacagctggt tgcgaaataa
     6421 ccacctgaac tatcaggaac tttcctgatc tggctgattg cataccaaaa caactttcgc
     6481 tacgttgctg gctcgtttta acacggagta agtgatgtac cgttatttgt ctattgctgc
     6541 ggtggtactg agcgcagcat tttccggccc gacgttggcc gaaggtatca atagtttttc
     6601 tcaggcgaaa gccgcggcgg taaaagtcca cgctgatgcg cccggtacgt tttattgcgg
     6661 atgtaaaatt aactggcagg gcaaaaaagg cgttgttgat ctgcaatcgt gcggctatca
     6721 ggtgcgcaaa aatgaaaacc gcgccagccg cgtagagtgg gaacacgtcg ttcccgcctg
     6781 gcaattcggt caccagcgcc agtgctggca ggacggtgga cgtaaaaact gcgctaaaga
     6841 tccggtctat cgcaagatgg aaagcgatat gcataacctg cagccgtcag tcggcgaggt
     6901 gaatggcgat cgcggcaatt ttatgtacag ccagtggaat ggcggcgaag gccagtacgg
     6961 tcaatgcgcc atgaaggtcg atttcaaaga aaaagctgcc gaaccaccag cgcgtgcacg
     7021 cggtgccatt gcgcgcacct acttctatat gcgcgaccaa tacaacctga cactctctcg
     7081 ccagcaaacg cagctgttca acgcatggaa caagatgtat ccggttaccg actgggagtg
     7141 cgagcgcgat gaacgcatcg cgaaggtgca gggcaatcat aacccgtatg tgcaacgcgc
     7201 ttgccaggcg cgaaagagct aacctacact agcgggattc tttttgttaa cccctacccc
     7261 acgcgtacaa ccgcgtgggg agacgacgcg gatttttaac tatgcgtatc ccccgcattt
     7321 atcatcctga accactgacc agccattctc acatcgcgct ttgcgaagat gccgccaacc
     7381 atatcgggcg cgtactgcgc atggggccgg ggcaggcgtt gcaattgttt gacggtagca
     7441 accaggtctt tgacgccgaa attaccagcg ccagcaaaaa aagcgtggaa gtgaaggtgc
     7501 tggaaggcca gatcgacgat cgcgaatctc cgctgcatat tcacctcggt caggtgatgt
     7561 cgcgtggtga aaaaatggaa tttactatcc agaaatcgat cgaactcggt gtaagcctca
     7621 ttacgccact tttttctgag cgctgcggcg ttaaactgga tagtgaacgt ctgaacaaga
     7681 agcttcagca gtggcagaag attgcaattg ctgcctgtga gcagtgtggt cgtaaccggg
     7741 tgccggaaat ccgtccagcg atggatctgg aagcctggtg tgcagagcag gatgaaggac
     7801 tgaaactgaa tcttcacccg cgcgccagta acagcatcaa tacgttgcct ttaccggttg
     7861 aacgcgtccg cctgctgatt ggcccggaag gcggtttatc ggcagatgaa attgccatga
     7921 ctgcccgcta tcaatttact gatatcctgt tgggacctcg cgttttgcgt acagagacaa
     7981 ctgcgctcac cgccattacc gcgctacaag tacgatttgg cgatttgggc taacggagaa
     8041 gaataatgat caagctcggc atcgtgatgg accccatcgc aaacatcaac atcaagaaag
     8101 attccagttt cgctatgttg ctggaagcac agcgtcgtgg ttacgaactt cactatatgg
     8161 agatgggcga tctgtatctg atcaatggcg aagcccgcgc ccatacccgc acgctgaacg
     8221 tgaagcagaa ctacgaagag tggttttcgt tcgtcggtga acaggatctg ccgctggccg
     8281 atctcgatgt gatcctgatg cgtaaagacc cgccgtttga taccgagttt atctacgcga
     8341 cctatattct ggaacgtgcc gaagagaaag ggacgctgat cgttaacaag ccgcagagcc
     8401 tgcgcgactg taacgagaaa ctgtttaccg cctggttctc tgacttaacg ccagaaacgc
     8461 tggttacgcg caataaagcg cagctaaaag cgttctggga gaaacacagc gacatcattc
     8521 ttaagccgct ggacggtatg ggcggcgcgt cgattttccg cgtgaaagaa ggcgatccaa
     8581 acctcggcgt gattgccgaa accctgactg agcatggcac tcgctactgc atggcgcaaa
     8641 attacctgcc agccattaaa gatggcgaca aacgcgtgct ggtggtggat ggcgagccgg
     8701 taccgtactg cctggcgcgt attccgcagg ggggcgaaac ccgtggcaat ctggctgccg
     8761 gtggtcgcgg tgaacctcgt ccgctgacgg aaagtgactg gaaaatcgcc cgtcagatcg
     8821 ggccgacgct gaaagaaaaa gggctgattt ttgttggtct ggatatcatc ggcgaccgtc
     8881 tgactgaaat taacgtcacc agcccaacct gtattcgtga gattgaagca gagtttccgg
     8941 tgtcgatcac cggaatgtta atggatgcca tcgaagcacg tttacagcag cagtaaccca
     9001 ccttagcgag aaggatctcg ttgagactct gagtgacagc gcccttcttt ccacgcatac
     9061 tggccgctgt tgcttttttg aaccaggaaa cagaacctct gacaatgaat ttacagcatc
     9121 actttcttat tgccatgcct gctctccagg atccgatttt ccgtcgttcc gtggtctaca
     9181 tttgcgaaca taataccaat ggtgcaatgg ggatcatcgt caacaagccg ctggaaaatc
     9241 tcaaaattga agggattctg gaaaagctga agatcacgcc ggagccgcgt gatgaatcaa
     9301 tccgtctgga taaaccggtt atgctcggcg gcccactggc tgaagatcgc gggtttattt
     9361 tgcatactcc gccctccaat tttgcttcca gcattcgcat ttcagacaac acggtgatga
     9421 ccacttcccg cgatgtgctg gaaacgctcg gcaccgataa acaaccgtct gacgtattgg
     9481 tggctctggg ctatgcctcc tgggaaaaag gtcaactgga acaagaaatt ctcgataacg
     9541 cgtggctaac ggccccggcg gatctgaata ttctgttcaa aacgccgatt gccgaccgct
     9601 ggcgcgaggc ggcaaaactg attggtgtgg atattctcac catgcctggt gtggcaggac
     9661 acgcctgatg agtggaacct tactcgcctt cgacttcggc accaaaagca ttggcgtagc
     9721 ggtcggccaa cgcattaccg gcaccgctcg ccctttgcct gcaattaaag cgcaggacgg
     9781 tacgccggac tggaacctca tcgaacgttt actgaaagag tggcagcctg acgaaatcat
     9841 cgtcggtttg ccgctgaata tggacggcac cgaacagccg ttaaccgcca gagcgcgcaa
     9901 atttgccaac cgtattcatg gtcgtttcgg tgttgaagta aagctccatg acgagcgtct
     9961 tagcactgtg gaagcccgtt ccggtctttt tgaacagggc ggctatcggg cgctcaacaa
    10021 aggcaaagtt gactctgcct ctgcggttat tattctcgaa agctatttcg agcagggata
    10081 ttaaggcgat ctaaaagcct ggcggatagt cgttaccgcc atcttgccgg atgcggcgtg
    10141 aacgccttat ccagcacaca tctggcagcg gctgtaggtc tgataagacg cgttagcgtc
    10201 gcatcagaca tttattgcct ttataggccc tataaacttg cgcatcgggc atggcaacgt
    10261 cacaaacgcc cttcccccac ccgctgctga taactctgct gaaacgttaa catccccacc
    10321 tgctgcccgg tttgaataat atgcggtaac tggtgggttt tcccttcgcg aatcaaattc
    10381 cccaccgcgg gagtgttaat cagtaattca aacagcgcca cgcgtccttc ctgtttatcc
    10441 acttccagct tttgtgacag cactgcccgt aaactacccg caagttgatt acgtacggga
    10501 tctttttcct gcgccggaaa tgaatccacc agtcgctcaa ctgcctgcgc ggcaccacgt
    10561 gtatgtaagg ttgccagcac cagatgcccg gtttctgccg ccgtcagcgc cagacggatt
    10621 gtctcactgt cacgtagctc tccgagcaaa atcacatcag gatcttcccg caatgcagcc
    10681 cgcaaccccg atgcgaacgt catacagtgc aaaccaattt cccgctgctg gatcaaacat
    10741 cgctggctgg catagagata ttccacagga tcttccagcg tcagaatatg cgcatcggca
    10801 tgttgattaa gatagccaac catcgccgcc agcgtggtag atttgccact ccccgtagcc
    10861 cccgtcacca gaatcaggcc attctcgctc ttgagtaatt ccggcaatac cggtggcgca
    10921 ccaagctgtt cgagctgtgg acagtgcgaa ggtaacaacc gtaatgccag agaaatacct
    10981 tgccgttgcg cgaacgcact gccacgcaac cgctggtttt ccgccagcga cacggcaaaa
    11041 tccagctggc cattttccag caatattgcc cgctgatcgt catccagcca ctcccgcagt
    11101 agctcttcga cgtccggcgc atcaaacggc gcagcttcca ttctgccacg aatgcgccat
    11161 cgtgcgggcc aggcgctgca caggtgtaga tccgagacgt tatgctttac actaagggcc
    11221 acaatttctt ccatattcat actaagatcc tcggaaaatg aacgatattg cgcataacct
    11281 ggcacaggtc cgggacaaaa tctcagcggc tgcaacgcgt tgcggccgtt ctccagaaga
    11341 aattacgctg cttgcagtca gtaaaacaaa acctgcgagc gccatcgcag aagccattga
    11401 tgccgggcag cgtcaatttg gtgaaaacta cgttcaggaa ggggtagata aaattcgcca
    11461 ctttcaggaa ctgggcgtaa caggattaga atggcatttt attggcccgt tgcagtctaa
    11521 taaaagccgc ctagtggcag agcatttcga ctggtgtcat accatcgacc gtttgcgcat
    11581 tgctacccgc ctcaacgatc agcgcccggc agaacttccc cctcttaacg tactgattca
    11641 aattaatatt agtgatgaaa acagtaagtc cgggattcaa ctggcagaac tggacgagct
    11701 ggcagcggcg gtcgctgaac taccgcgttt acgtctgcgc gggctgatgg caatccctgc
    11761 gcctgagtca gaatatgtaa ggcagtttga agttgcacgc caaatggctg tagcatttgc
    11821 cggactgaaa acgcgctacc cgcatatcga cacgctctct ctgggaatgt cggacgatat
    11881 ggaagccgcc attgcggcag gtagcacgat ggttcgtatc ggcactgcaa tttttggtgc
    11941 gcgtgattac tctaaaaaat aaggaattaa aggaacgcca tgaatacgtt gactttcctg
    12001 ctttcaacgg tcattgagct gtataccatg gtgctgttat tacgcatctg gatgcagtgg
    12061 gctcattgtg atttttacaa ccccttctca cagtttgtag tgaaggtagc gcagccaatt
    12121 atcgggccac tgcgccgcgt tattccggca atgggtccca ttgacagcgc ctcgctgctg
    12181 gttgcctata ttctcagttt tatcaaagcc attgtgctgt ttgaagtggt gaccttcctg
    12241 ccaatcatct ggattgccgg tttactgatt ctgctgaaaa ccatcggctt gctgattttc
    12301 tgggtcctgc tggtgatggc gattatgagc tgggtaagcc agggtcgtag cccgattgaa
    12361 tacgtgctga ttcagctggc cgatccgctg ctgcgcccga ttcgccgctt gctaccggca
    12421 atgggtggga ttgatttctc gccgatgatc ctcgttctgc tgctgtatgt catcaatatg
    12481 ggtgtcgcag aagtattaca ggcaaccgga aacatgctgc tgccggggct gtggatggcg
    12541 ttatgagtgc cgtaacagtt aatgatgacg gtctggtttt acggctctat attcagccga
    12601 aagccagccg tgattctatt gtcggtttac atggcgacga agttaaagtc gccattaccg
    12661 cgccgccggt tgacggccag gccaacagtc atctggtgaa gtttctcggt aagcaattcc
    12721 gggttgccaa aagccaggtg gtgattgaaa aaggcgaact tggccgccac aaacaaatta
    12781 aaatcattaa tccgcaacaa atcccgccag aaatcgcggc gttaattaat taggtatcct
    12841 atgcaaaaag ttgtcctcgc aaccggcaat gccggtaaag tgcgtgagct ggcgtcgctg
    12901 cttagcgact ttggtcttga tatcgtggcc caaacagacc tcggcgttga ttccgctgaa
    12961 gaaaccggcc taacctttat cgaaaacgcg attctgaaag cgcgccatgc ggcaaaagtg
    13021 accggtttac cggcaattgc cgacgactct ggtctggcgg tagatgcgct gggtggcgcg
    13081 ccgggtattt actccgcgcg ttattccggt gaagacgcga ccgatcaaaa gaatctgcaa
    13141 aaactgttgg aaacactgaa agacgtaccg gacgaccaac gtcaggcgcg tttccactgc
    13201 gtgctggtat atctgcgtca cgcggaagat ccgactccgc tggtatgcca cggcagctgg
    13261 ccgggcgtga ttactcgcga accagcgggc actggtggct ttggttatga tccaatcttc
    13321 ttcgtacctt ccgaagggaa aaccgccgcc gaaatgaccc gcgaagaaaa gagcgccatt
    13381 tcccaccgtg gtcaggcgtt gaaactgctg ctggacgctt tacgtaatgg ttaaattacc
    13441 gccgctgagt ctctacattc acatcccgtg gtgcgtgcag aaatgcccgt actgcgattt
    13501 caactctcac gcgttgaaag gagaagtgcc gcacgacgat tatgttcagc atctgctttg
    13561 cgatctggac aatgatgtgg cttacgctca gggccgtgaa gtaaagacaa tctttattgg
    13621 cggtggtacg ccgagcctgc tttccggccc ggcgatgcaa acgctgctag acggcgtgcg
    13681 ttcgcgtttg ccgctggcag cggatgcaga aattactatg gaagcgaacc caggcacggt
    13741 agaagcggat cgctttgtcg attatcagcg tgctggtgta aaccgcatct ctattggtat
    13801 gcagagtttt agcgaagaaa agctgaaacg acttgggcgt attcatggcc cgcaagaagc
    13861 gaaacgagcg gcaaagctgg cgagcggttt agggttacgt agctttaacc ttgatttgat
    13921 gcatggacta ccggatcaat cactggaaga ggcgcttggc gatctacgcc aggccattga
    13981 actgaatccg ccgcatcttt cctggtatca actgaccatc gaacctaata cgctgtttgg
    14041 ctcgcgaccg ccggtgctgc cggacgatga cgcgctgtgg gatattttcg aacaggggca
    14101 tcagttatta accgcagcgg gttatcagca atatgaaact tccgcttacg ccaaacccgg
    14161 ttatcagtgc cagcacaatc tcaactactg gcgctttggt gactacatcg gtattggctg
    14221 cggcgcgcac ggcaaagtga ccttcccgga tgggcgcatt ctgcgtacca ctaaaacgcg
    14281 tcatcctcgt ggttttatgc aaggaaggta tctggaaagc cagcgtgatg tcgaagccgc
    14341 agataagccg tttgagttct ttatgaatcg cttccgtctg ctggaggccg cgccgcgcgt
    14401 ggagtttatt gcgtataccg ggctttgcga agatgtgatt cgcccacagt tagacgaggc
    14461 gattgcccag ggttatctca ccgaatgtgc ggattactgg cagataacgg aacatggtaa
    14521 attattctta aactcacttc ttgaattatt cctcgctgag taatcattaa tgcgcctgcg
    14581 ggcgcattaa tcacttcttt tatccccccg ttaattaacc tcggcagaaa tatatttctt
    14641 actgaataaa tatcgacgac atttattata tatatttagt gaatatttac taatcattca
    14701 ttgattataa atatgtacaa cctggcgcac aaatttaata tttaatttct gactttattt
    14761 caagttgtga taaacataga caagttgtca gacaggttaa ccaatttgtc gcacaaagca
    14821 ctgataagca caattagata tcaaccaatt tccatccttc aacagggagt ttttttatga
    14881 aaatgaggtc atttacccgc tcattagtct gcgcatcctt gctggctctg gttagcactg
    14941 gcgtaaacgc cgcagaaaaa gtgacgttaa agttagcaca caacctggaa agaagtcatg
    15001 ttgtacatca gagttttgaa gagctggcga aagaggtaaa acagctgtca aaaggtaata
    15061 tggtgatccg tatttatccc agcagccaga tgggtaatgc gcgagaaact atggaattgt
    15121 tgcaaaatgg cgcactggat atgaccaaag gttccgccag tgatctggag tcttttgata
    15181 atatttatgc tatctataat ttgccgttct tattaagttg tttttggtga agttggtaaa
    15241 gaaattatgg actcaacaaa aggcaaaggc ttttttgctt tatcagctta tgtagcgggt
    15301 actcgcagtt tctatgcgaa aaaaccgatt acaaagccag aagatcttaa aggactgaaa
    15361 attcgtgttc agccaagtcc gaccaccatc aaaatgattg aactgatggg tggttcaccc
    15421 actccaattt catttggcga agtttatact gcgatgcagc aaggtgtagt agatggcgca
    15481 gaaaataacg tgccttcctg gatgcagact cgacatattg aaattgccaa agttttttct
    15541 gaagatgaac acgcctcaat tcctgacttt ttggttatct ccaccaaaac atggaataaa
    15601 ttacccccag aacagcaaca aatcctcgaa acagcggcga agaaatccga agcttatcaa
    15661 caaaaattgt gggaaaaaat tgacgctgac acacgtgctc aggcgaaagc tatggggggt
    15721 gaaattgtaa aagtggataa agcgccattc cgtaaagccg tgcaacctct gtttgacgac
    15781 ttcaaaaaag atccaaaaca agctgctttg ttagagaaat tcgacaacgc tgctcaataa
    15841 tattccgggc cagcctctgg ccctgatttt ccaggatgct tacaatgata tttaaccgcc
    15901 tgaaacttgc ggtagaccgc gtcattgccg cgttttctgt cgccgtaatg cttgctctgg
    15961 ttgtatgcgt agtctggcaa gtatttagcc gctacgtact taaccagcca agtacattaa
    16021 ccgatgagct ggcacgcttt ttgatgatct gggtaggatt attaggtgct gcatataccg
    16081 tcggcgcgca gcgacatctc tctattgatt tatttgctct cgccctgaat aagcgcaaac
    16141 aattacttct tagtatagtc attaacgttc tcattttagg ttttgccggt tcggtcattg
    16201 tgactggtgg cctgaaatta attgataaaa cactggcaac atcacaggta tctgccgcta
    16261 tgcaaatacc gatgggatac gtatatatca ttctgccatt aagtggagtg gtaatgatgt
    16321 tttatgc
//
LOCUS       DAETPZ010000085        16030 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.085, whole genome shotgun sequence.
ACCESSION   DAETPZ010000085 DAETPZ010000000
VERSION     DAETPZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.085"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="K0W59_003113"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="K0W59_003113"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HBI3024050.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="K0W59_003114"
     CDS             1020..1703
                     /locus_tag="K0W59_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HBI3024051.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="K0W59_003115"
     CDS             1700..2161
                     /locus_tag="K0W59_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBI3024052.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="K0W59_003116"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="K0W59_003116"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBI3024053.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHLESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIL
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="K0W59_003117"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="K0W59_003117"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HBI3024054.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSRAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSAFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="K0W59_003118"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="K0W59_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HBI3024055.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="K0W59_003119"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="K0W59_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HBI3024056.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            5380..6210
                     /locus_tag="K0W59_003120"
     CDS             5380..6210
                     /locus_tag="K0W59_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HBI3024057.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYFGK
                     "
     gene            complement(6351..7124)
                     /gene="uxuR"
                     /locus_tag="K0W59_003121"
     CDS             complement(6351..7124)
                     /gene="uxuR"
                     /locus_tag="K0W59_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HBI3024058.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(7339..8799)
                     /locus_tag="K0W59_003122"
     CDS             complement(7339..8799)
                     /locus_tag="K0W59_003122"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HBI3024059.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGSAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(8880..10064)
                     /gene="uxuA"
                     /locus_tag="K0W59_003123"
     CDS             complement(8880..10064)
                     /gene="uxuA"
                     /locus_tag="K0W59_003123"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBI3024060.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            10404..11747
                     /gene="gntP"
                     /locus_tag="K0W59_003124"
     CDS             10404..11747
                     /gene="gntP"
                     /locus_tag="K0W59_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HBI3024061.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(11998..12900)
                     /gene="fimH"
                     /locus_tag="K0W59_003125"
     CDS             complement(11998..12900)
                     /gene="fimH"
                     /locus_tag="K0W59_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HBI3024062.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPTNNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(12920..13423)
                     /gene="fimG"
                     /locus_tag="K0W59_003126"
     CDS             complement(12920..13423)
                     /gene="fimG"
                     /locus_tag="K0W59_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HBI3024063.1"
                     /translation="MKWCKRGYVLAAMLALASATIQPADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(13436..13966)
                     /gene="fimF"
                     /locus_tag="K0W59_003127"
     CDS             complement(13436..13966)
                     /gene="fimF"
                     /locus_tag="K0W59_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HBI3024064.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(13976..>16030)
                     /gene="fimD"
                     /locus_tag="K0W59_003128"
     CDS             complement(13976..>16030)
                     /gene="fimD"
                     /locus_tag="K0W59_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HBI3024065.1"
                     /translation="SVQNRIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSG
                     SKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRG
                     FAPVIHGIAHGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGS
                     TQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYG
                     GTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNES
                     GTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGK
                     LQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAW
                     QKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLED
                     NNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVL
                     AHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVAL
                     DTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSE
                     SSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAE
                     CR"
BASE COUNT         3861 a         4136 c         3923 g         4110 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagcc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tgactgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct ttttttccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg tggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc tggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattct
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccgg gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggg gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagcg
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtaag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaac cgctagtata ttctgaatat tccgaatagt tacgtagatg
     4981 aaaatacttg tcaccgctgc tacttaattc cggtgggaaa ttatttattt ccgcataatc
     5041 agctatgtat tatttgaaac aattagtttg taaataatat aaattgataa catatcaaat
     5101 acatcaaaaa taccattcac aggcattatt aatatagacc tcaatatagc aacatcaaat
     5161 tccaattagt aataagatat gcttttaaat tgcatgcaaa taattttaaa tgagcgtttt
     5221 ttgatacttt agagtgtttc ctacccttta atatcactgt gtagagttga tatcactaca
     5281 acagaaacat gcatgtcatt tgtttacttc tctttttact acagaaaaat ttatctattt
     5341 ttctaaacat cagttaaata ctcataaaag gtaactatta tgtctatgcc attaagcaat
     5401 gccgctaata accaattcaa tacaaataat cacttccttc atcatccaaa agtcgacagt
     5461 gagttaacac gtcgatatga atatgcacgc ctggatacgg aaaatatcta tcttctgcct
     5521 cttgctcgtg gcaataacca taattacgat ggaaaaagcg ttgtcgaaat tagaaaactt
     5581 aatattggaa aacagtcctg gccatttaat tatgtgacag atacatgcag agaattcaat
     5641 ggtattacaa caacaggacg tatgctctat cgaaacttga aaatcacctc agctttagac
     5701 gaaatatatg gtggtatatg taaaaaggcg catgctgcta cagagttagc agaaggttta
     5761 cggctaaatt tatttatgaa gagccctttc gatcctgttg aggactatac agttcatgaa
     5821 ataacattag gccctggttg caatgttcct ggttatgccg gaaccacgat aggatatatt
     5881 tcaacccttc cagcttctca ggcaaaaaga tggaccaatg agcagccaag aattgatatc
     5941 tatattgatc agataataac tgtctcaggg gtagcaaatt cttccggatt cgcccttgcc
     6001 gcattactta atgctaatat tgagatggga aatgacccaa tcattggtat agaagcctat
     6061 cctggtaccg cggagatcca ttcaaaaatg ggatataacg ttatccccgg tgatgaagat
     6121 gcgccactca aacgcatgac tctgcaaccg tcatctcttc cagaactgtt tgaattaaaa
     6181 aatggtgaat ggaattattt tggtaaatag ccactgagaa gaatgagtac taatattcag
     6241 gttatacaga tatttaaaca aaaccctgca atttacaaag taatactcat tgcgacttct
     6301 taatttaaga aagcgcaagg aacgtttacc cttgcgctta ttataataag tcaggcgtcg
     6361 actttatcca gcggccatga atcaaagaga tagccgtcaa aataaatatc gtcaacgttc
     6421 gagaattcca gcagacgttg cttaacgttt tccagatgct gccacattgc cagcttcgcc
     6481 gctcgggcat cttttttgat taacgcggcg aggatctgtt tgtgatcgcc caaccactct
     6541 ttgcgataca ggctgtcatc cagatggctg tgcaactgga tccacattgg attgttttcc
     6601 cgccactgcc aggactgacg gaataactcc accagcatgc tgttatgcgt tgcttcagca
     6661 atggcgagat ggaactgcat gtcaccgctt tcgctgctac ccggcgcgct ggaagccagt
     6721 tcacgctctt ccagttgcaa tgcctgacgc attttgacga tatcttcgcg ggtagcctgc
     6781 agtgcggcaa actcggcgat gttactttcc agtaactgac gcgcctgtaa cagctcaaaa
     6841 ggacccgcat cattgcagac gttggcatcc ggactgtctg tgttgtggct gcctgagcta
     6901 tcaagcacat agataccggc accccggcgc acttccacca gccctttgat ctccagcatg
     6961 atcagcgctt cacgtaccac cgtccgcgtg acatcaagca tttctgcaat ttcacgctcc
     7021 ggcggcagcc gttcgccagg attgtacggc gtctttatga tcagatcgcg gatcatcgcc
     7081 ccgacttcct ggtaaggtct ttgcgcagag gtgacagatt tcatggtgtt gtgtccgtta
     7141 ggtgcggtta atcaaatttt tgccggatgc ggcgtgaacg ccttatccgg cctacaacgg
     7201 gcaattttac ggcccccgtt aatctcaaac tataccatta gcactggaag ttggcacgta
     7261 caggtgggca aaaaaacgaa aactggtcaa ccaaatatca gtttgattga ccagtaagtc
     7321 tgtagggtaa tcagttagtt acagcgcagc cacacactcg cgcgcaccgc gttcgcacag
     7381 ctgctgatat gccgccgtca ccgcgccaac aaagtcggca ttctgcggca gatcatcggc
     7441 aaaaataccg ctcaggccca gcagcgcttt cacgcggtct gcgccctgat actgcgcgtt
     7501 aatcttctgg aactcggcca gcatcgggtc gaccacgtca atggcactac cctgctcatc
     7561 cacgccctgg gtgtaacgca tccagccagc cacgcccagc gccaggtgac gccagctgcc
     7621 gccgttttgc aggtgcagac gcaccgggtc cagtagacgc tgcggtaact tctggctgcc
     7681 gtccatcgca atctgccagg tacggtgacg cagagacggg ttgctgaaac gctcgatcag
     7741 cagcgtcgca taggcgttca ggtccgtacc ttccggcatc gacagcgttg gcgcttgttc
     7801 ctgcatcatc agggcaaagg ccgctttgcg ataatccggg ttagtcatgg tgtcggcaat
     7861 ggtttcatag ccgccgaggt aaccgaggta cgccaggaaa gagtggctgc cgttcagcat
     7921 acgcagcttc atcatttcga acggcacaac gtctgcaacg aactgtgcgc ccactttatc
     7981 ccagtccggg cgaccattaa cgaagttatc ttcaatcacc cactgacgga acggttcgca
     8041 ggcaatggcg cacgggtcgt aaacacccag ctggtcagca atttcctgta aggtttctgg
     8101 cgtcgccgcc ggaacgatgc ggtcaaccat ggtgcacggg aaggtgacgt tctcttcaat
     8161 ccatgccgct agctgcgggt cacgcgcctg agccagcccc agtaccgcga ccttcgccac
     8221 atgaccgttt tcacgcacgt tatcgcagga catcaccgta aatgctttca gccctttttc
     8281 acgacgcaga cgcaaggctt cgacgatgta accaatcgcg gacttcggcg cagtcgggtt
     8341 ttccagatcg tgcttgatca gcgggttatt gagatccagt tgaccgcttg ccgcatcagc
     8401 gcagtagcct ttttccgtga ccgttagaga gacaatcgcc gtttgcggac gcgccatcgc
     8461 gttgagaata ccttcgcagc cgtcgatttc cggatgcagc gcttctttca tcgaaccgat
     8521 aattttcagc tcggtgcttt ctgcgccttt ttccgctacg gtgtacagca gttgctgttt
     8581 tttcaggttt tcgatcagca tgcggtcgtt gcctggcatc aggttaactt cgcagatgcc
     8641 ccagtcgctg tcggtgcttt ccagcagatg atgggtatac agcgcctggt gcgcgcggtg
     8701 aaacgccccg caaccgagat gcacaatgcg tgattccaga cgagaatgat cccatgacgg
     8761 gcgggcaacc ggcagattgc tgtcaacaat agtagtcatc gtaaactcca gaatccgtct
     8821 tcagagagtg acggcaagga agaaggaacc ggcacggcgc gcagccatgc cggtgggtat
     8881 taacggctaa agaaagcgcg ctggatcgcc agttcgacac cgcgaacttc ggccaggcct
     8941 ttcagacgac caattgcgga gtaaccaggg ttggttttct tcttcaggtc gtccagcatc
     9001 tgatgaccgt ggtccggacg catcgggatc aggtcttctt tgccttccgc tttacgacgg
     9061 tgttcttctt caacaatcgc tttcaccact tcgtacatat caacgtcacc gttcaggtgc
     9121 gccgcttcgt ggaaggtttt cgggttatct tcacgcatgg tggagcgcag atgggtgaag
     9181 taaatacgcg gaccgaactg cttgatcata tcaaccagat cgttgtcagc acgcacgccg
     9241 taggaaccgg tgcacatggt gaaaccgttc gccatgctgt ttacggtatc aaccatccac
     9301 tgcatatctt caatggtgga aacaatgcgc ggcaagccga ggatcgggcg cggcggatcg
     9361 tccgggtgaa ccgccatacg cacgccaact tcttcagcaa ctggaataat cgctttcagg
     9421 aagacggcaa agttttcgcg cagtttggct ttgtcgatat ctttgtacag ctccaggtgc
     9481 ttacggaact ggtcgagggt atacccttct tccgcgcccg gcagaccggc aatgatgtta
     9541 cgcgtcagac gcgctttgtc ttcgtcgctc atagtggcga agcgttcagc tgcctgagca
     9601 atttcttctt cggtgtaatc cgcttccgcg cctggacgtt tcaggatatg catttcgaat
     9661 gcggcgaatt cgatctggtc gaagcgcaga gctttggagc cgtctggcag cacgtattcg
     9721 aggtcagtac gggtccagtc gagcaccggc atgaagttgt agcacacggt gcgaatgcca
     9781 cactgcgcca ggttgcgcag agtctgctga tagttagcga tccactgctc atagttgcca
     9841 gtgtgggttt tgatatcttc gtgaattggt acgctttcta cgacagacca caccaggcct
     9901 gcgtcttcaa cgatcgcctt gcgtttgagg atctcttcta cggaccatac ttcgccgttc
     9961 gggatatggt gcagcgcggt aaccacgcca gttgcgcccg cctgacggac atcagctaaa
    10021 gaaaccggat cgtttgggcc gtaccagcgc caggtctgtt ccatgatttt tcctctttca
    10081 catagtgggt tccacagcaa agagacgcgg aaccttggag ttgtttaggt atccggatga
    10141 acaaccagac cgcagccgtg gcgttgcatc ggatgagatg gcgtataagt tctacctttt
    10201 ggtaagacat gtcaacccga actctgaaat tggttaacca catcacaaga atttcactct
    10261 catggttgtt gctgcaaatt gcagcaaagg gtaatcgatg gttattgatc agaggatgtg
    10321 acattcatcg caacaatggt tgaccaattt gcataacatg tcgcccaaaa taacaacggt
    10381 tcaaccaagt aaggtaaaac gttatgcatg tgcttaacat tctctgggtg gtattcggca
    10441 ttggcctgat gctgatactg aatttgaagt tcaaaatcaa ttcaatggtg gctttgttgg
    10501 tggcggcgct gtccgtcggg atgctggcag gcatggattt gatgtcgctg ctgcacacca
    10561 tgaaagcggg cttcggcaac acgctgggag aactggctat catcgtggtg ttcggtgcgg
    10621 tcatcggtaa attgatggtc gactccggcg cggctcacca gatagcacat acgctgctgg
    10681 cgcgtctagg tctgcgctat gttcagctgt cggtgattat catcggcctt attttcggtc
    10741 tggcgatgtt ctatgaagtg gcctttatca tgttagcgcc gctggttatt gttattgccg
    10801 ccgaagctaa aattccgttc ctgaaactgg cgatcccggc agtagcagct gccactaccg
    10861 cacattcact gttcccaccg cagccgggtc cggtggcgct ggtgaatgct tatggcgcgg
    10921 atatggggat ggtttatatc tatggcgtac tggtgacgat cccaagtgta atctgcgcag
    10981 gtctgatcct gccgaagttc ctcggcaatc ttgagcgccc aacgccatca ttcctgaaag
    11041 cagatcaacc ggtagatatg aataatctgc cctctttcgg cgtttcgatt ctggtgccgc
    11101 tgatcccggc gatcattatg atctctacca ccatcgccaa tatctggctg gtaaaagata
    11161 cccctgcctg ggaagtggtt aactttattg gttcctcgcc gattgcgatg tttattgcga
    11221 tggtggttgc attcgtactc tttggcaccg cgcgtggtca tgacatgcag tgggtgatga
    11281 acgcttttga aagcgcggtg aagagtattg caatggtgat tctgatcatc ggtgcgggtg
    11341 gcgtgctgaa gcagaccatc atcgacaccg gcattggcga caccatcggc atgttgatgt
    11401 cccacggcaa tatctcgccc tacattatgg catggctgat cactgtgcta attcgtctgg
    11461 cgacgggtca gggtgtcgtt tcggcgatga ccgccgccgg gattatcagt gctgcaatcc
    11521 ttgatccagc aaccggtcag ctggttggcg tgaatccggc gctgctggta ctggcgacgg
    11581 ctgcgggttc caacaccctc acccacatta atgatgcatc tttctggttg ttcaaaggtt
    11641 actttgacct gtcagtaaaa gacacgttga aaacctgggg tctgctggag ctggtcaact
    11701 ccgtggttgg gctgattatt gtgctgatta ttagcatggt agcgtaaaag aacaccagag
    11761 cctgccaatg gcaggctcag actgatgact aaagtctaaa aatgtgcccg gacagtcccc
    11821 tcgcccctcc ggggagaggg ttagggtgag gggaacagac cagtaaagtg cgctttgttt
    11881 atgccagatg cgacgctgac gcgtcttatc tggcctacaa agggctaacg tgcaggtttt
    11941 gtaggtctga tagcgtagcg cctcaggtac cagcattagc aatgcgctgt aattttttta
    12001 ttgataaaca aaagtcacgc caataatcga ttgcacattc cctgcagtca cctgccctcc
    12061 ggtacgtgcg taatttgccg ttaatcccag acttaccgcc gaagttccta ctgctcctaa
    12121 cgataccgtg ttattcgttg gaataatcgt accgttgcgc gtcaactgta cgccgacgcc
    12181 ctgcgctggt gaaaacgacg cggtattggt gaaaatcgag ttgcccgcat ctgcggttgt
    12241 gccggagagg taatacccca ggttttggct tttcgcacaa taaacggtaa gaggaattgg
    12301 cactgaacca gggtagtccg gcagagtaac ggtgacatca cgagcagaaa catcacaacc
    12361 gccagtgggc accaccacat cattattggc gtaaatattc cacacaaact gaaaatcatc
    12421 gctgttatag ttgttggtct gtcgcaaaat aagcacggca attaatgaac cagctttaat
    12481 cgccaccccg cccgcactgc tcacaggcgt caaataaagc gccaccggcc acggcttatc
    12541 cgttctcgaa ttataaacaa cccgcggcgt ttcgctggtg gtcggaaatg gatagctact
    12601 gccactatat tttacggtcc cggaaaaatt agataacacg ccgccgtaag ccgagcctcg
    12661 ttgcagtgtg acatagtctg taatggtttc cggataatcg ttatggcaaa agatttgcgt
    12721 cgaaagatct acgaccaggt tttgccccac attcacgaca ggcgcaaggt ttacataaac
    12781 attagcgctg ccaccgccaa tagggatagc ggtaccattg gcggttttac aggcgaatga
    12841 ccaggcattt accgaccagc ccatcagcag tacagcaaac agggtaatag ctcgtttcat
    12901 tacaattatc tcttcgggtt cagctgtagg tataggtgat gctaatcact gcctgaatgg
    12961 ttccctgagt ggctccgcca tttaccgtca atgctctgac ctgtaacggg aagtgcgctg
    13021 attgtgagga atcatccacc tgaactgttt tggttgcgcc agtattcaat gtgttgccac
    13081 tgtcatcctg tagctctaac tggatgtttt gcgcggtccc ctggttttta taatatccgg
    13141 tgctgtcggc tgccccgctg aagctggcag tgaccctcga cgttcccacc ggacaattag
    13201 tcaactcaag cgcaacatca tgccaggccg atgccgcccc ggcagacata agactgaaag
    13261 aataaagatc gccgagatct accgtggcat tggtggtgga aaccgtacac ggtttggcga
    13321 cgaccttacc gttcaccgtg atggtgacat cggctggctg tatcgtcgca cttgcgagcg
    13381 ccaacattgc cgccaataca tacccacgtt tgcaccattt catgagcatc tccagttact
    13441 gatattcaag agtgaaggta gccgtagcat tgatatgccc cgcagtgaca ggcacctgtg
    13501 tcgccattag ccgggcgtaa aaattcagcg tatttggttt acccggcgtc agggtcgtcc
    13561 acgaaatcgc agacgatgga gcattaaggg gtatttgatt ttgctgctca ttcagaagct
    13621 gtattcccag tcccgcagcc gctgacaccg tattttcaag tgcaagcagg ttggcattgt
    13681 ggctatctgc aacgccggta aacccaactt ttacggcaga aacggcatta ccacagggtg
    13741 acagcaaaat acgaaatgga acgacaggag tcgtcgcgcc aatgttgtta aattgcttcg
    13801 ccgcgttttc catcagatca acagtaaaat tggttgattc agcggccaca ctacagccgt
    13861 tatctctgac atagccgcgg atagtaatcg tgctatccgc agccaaaacg cgacttaccg
    13921 ccagccacaa aaaagcgcac agaagataaa aaggtttgtt tctcatcacg cccccttaac
    13981 gacattcagc tgatagctgg gttaataact gctgctgact ctctggtggc agttgataat
    14041 tggcgacaca gtgagcattt tcctcttctc cccatttcac ctgaactttt cccgctaaag
    14101 gcattccgct gaggtaaacc tgaccattat ccgcaacaat gccgctactc tggctactct
    14161 ctgatgtcac catcgcccca aacggcagcg gcttattatt gtgagttagc gtcatgagca
    14221 gttttatccc aacgcgcgct ttaaactctg ctcgcacgat cgccccacga gtgggaacaa
    14281 cgttagcgac cgcgttatct aaatcgacgt tatcagccaa ggtattggta tccagcgcca
    14341 ctctattttc ccgatattca gtggcataag gcagcacggc ataaccacgc cagtcggtac
    14401 gcacccccgt ctggttttcg acttttgcat cttttgcgcc aggcgcttta acaagcacca
    14461 ccgtatcgtt taacggctgc cccagcgtta cgccattggc atgagccaat accccaccgc
    14521 tgactccgta atagagctgc ttaatatcat cgctatggct gtaaccgata ttggcattgc
    14581 cgtaaccacc gcgataattc agcgtggcgt agcctgtact tccgctatta ccatcgcctc
    14641 ccccggcata gccggtttgc acgctataac tgaggttgtt gtcttccagc aacgtaccgt
    14701 atacaccagc cagattggtc atccgaccgt tgagatcgtg tgacatgctg tagctggcac
    14761 tggcatgtcg ccactgagat ttactgtcag aacgcagcca gtggctgaaa ggaatattga
    14821 cgttaagcgc taacatctga tcccgtcctt tttgccaggc gtttttcgtc aggctatagc
    14881 tgagcgtcca gttgatatct tcgaacgcag tatttaatcc agcctggaat tgctcatcga
    14941 cattactcgt tccccaataa gtttgatggc taccactcaa atacagtgtt gatgtgcgcc
    15001 cgagttgctg agtaacggtg agctgtaatt tcccgcgttt gttataagcg aggttgtaat
    15061 agtcggtgaa tttcggctta acctgaataa ctccatcctg tgtttcgatg ttgtagccat
    15121 tcattcgact gtatgttgta tcagcgaaat taaaatatcc gctggtcgaa taacggtaac
    15181 ccactaactg aatattcgtg cctgattcat tgagcgattt gttatagaga aaacgcaccg
    15241 attgtccgtc atgctgactg tcatcgggaa gtgtggaatt agcctgcgtc atatccacag
    15301 acagagcgcc cagtgcctcc atgtttttcc cgataccgaa attaaaagca cgataacgat
    15361 ccgccagttg cgttccacca tatattgtcc agccagccgg aaggccgtgg agtaatgtac
    15421 tctggaaaaa gcggggtttt tcctgctgcg catttccact acggtattct cctgccgtaa
    15481 tggaataacg agtatgccct tcacgttgca aaagcgggac tgacgaatag ggtacggtaa
    15541 aaatctgcgt gctgccgtca gcctctttga tcgttacctg caagtcacca ctattacctg
    15601 cggcatagat atcattgatg gtaaaaggcc ccggtggcac cgtactatta taaatgccat
    15661 atccattttg tttaatagtg acctgtgcag taccatgagc aataccgtgg atcaccgggg
    15721 caaatcctct ttggctatcg ggtaacatat tgtcatctga ggccaattgt gcgccgcgaa
    15781 agttaatacc atcgaaaata tcgccctggg tataaccatc acccaacgtc agccgggaac
    15841 gtaaaggaat tatgtctcgc tcaatccagg taatgatatg ctgccattta tttttgctac
    15901 ctgatgaact gtcgctactg ttataactcc aggtggtatt gtcgcgtaaa cgccacgcac
    15961 caatatttaa cccactctgt aggtttaaat atgcataatg gctgttaccc ccaatccgat
    16021 tctgtacact
//
LOCUS       DAETPZ010000086        15955 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.086, whole genome shotgun sequence.
ACCESSION   DAETPZ010000086 DAETPZ010000000
VERSION     DAETPZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.086"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(19..1014)
                     /gene="yjfF"
                     /locus_tag="K0W59_003129"
     CDS             complement(19..1014)
                     /gene="yjfF"
                     /locus_tag="K0W59_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HBI3024066.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(1001..2023)
                     /locus_tag="K0W59_003130"
     CDS             complement(1001..2023)
                     /locus_tag="K0W59_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3024067.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADICGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2037..3539)
                     /locus_tag="K0W59_003131"
     CDS             complement(2037..3539)
                     /locus_tag="K0W59_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBI3024068.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3679..4636)
                     /gene="ytfQ"
                     /locus_tag="K0W59_003132"
                     /pseudo
     CDS             complement(3679..4636)
                     /gene="ytfQ"
                     /locus_tag="K0W59_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4946..5476
                     /gene="ppa"
                     /locus_tag="K0W59_003133"
     CDS             4946..5476
                     /gene="ppa"
                     /locus_tag="K0W59_003133"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HBI3024069.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5856..6197)
                     /locus_tag="K0W59_003134"
     CDS             complement(5856..6197)
                     /locus_tag="K0W59_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HBI3024070.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6200..9979)
                     /gene="tamB"
                     /locus_tag="K0W59_003135"
     CDS             complement(6200..9979)
                     /gene="tamB"
                     /locus_tag="K0W59_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HBI3024071.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGIYTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9976..11709)
                     /gene="tamA"
                     /locus_tag="K0W59_003136"
     CDS             complement(9976..11709)
                     /gene="tamA"
                     /locus_tag="K0W59_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HBI3024072.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGGGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11915..12553
                     /gene="msrA"
                     /locus_tag="K0W59_003137"
     CDS             11915..12553
                     /gene="msrA"
                     /locus_tag="K0W59_003137"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBI3024073.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGLYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12876..14219
                     /locus_tag="K0W59_003138"
     CDS             12876..14219
                     /locus_tag="K0W59_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBI3024074.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14281..14487)
                     /locus_tag="K0W59_003139"
     CDS             complement(14281..14487)
                     /locus_tag="K0W59_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HBI3024075.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKMHLSVMSEVNRQVMRLQTEMA"
     gene            14812..15366
                     /locus_tag="K0W59_003140"
     CDS             14812..15366
                     /locus_tag="K0W59_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBI3024076.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15429..>15955)
                     /locus_tag="K0W59_003141"
     CDS             complement(15429..>15955)
                     /locus_tag="K0W59_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024077.1"
                     /translation="ACDILQKKQSDIPKRIKGYIRQAFGDVSFGYALLEDVVSKLKRG
                     KFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLGFALKIYGGDERFRNGSSVVLED
                     EDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSNITKEKICEKYKSIIEAFKKEQ
                     LALPVLVRCKNSET"
BASE COUNT         3797 a         4373 c         3921 g         3864 t
ORIGIN      
        1 ccacacggag aggcattttt atcgctgcgc aatgttgact cttgtcactg gcgaactctg
       61 acgattctcc cacagcaccg ttaacccacg ctgtaatgct ataaaaataa acaacaaaat
      121 accgatggcg attttcgtcc accaggaact cagcgtgccg tcaaagttga tgtaagtctg
      181 gatcagtccc tgaattgcca cgccaaaaag cgtcccaagc accgtcccga cgccaccact
      241 taacaacgta ccgccaatca ccaccgacgc gatggcgtcc agttccacac ctacgcccgc
      301 cagtgcatat ccggcttggg tataaatcga gaagacaatc cccgccagtg ttgccagtcc
      361 ggtggagagc atataaatgc gaatagtggt gctgcgagta gaaatcccca tcaggttcgc
      421 cgacgtcgcg ttgccgccaa tagcgtacac ctgattgcca aaacgggtac gatgcgcaag
      481 gaaaataccg ataaccacca ccgccagcat caacagcccc atcgcactaa ggcgaccgcc
      541 gccggggatt ttccacgcaa ggcttgagag cgtgtcataa atgggatggt ttatcggaat
      601 cgactcttcc gaaacgagat agctgacgcc gcgcaaaaag aacatccccg caagcgtaat
      661 gataaatgcc gggatcttca gggcgtcgat cagaagcccc ataaatgcgc cgaaggcaca
      721 gcccatcacc agcactagcg gaaacgccag cagcggcgag aggccgaaat cgccaaccac
      781 ttttgccaga aacacgccag taaaggcgat caccgaaccg acggagagat cgataccgcc
      841 agagaggatc acaaaggtca tgccaacggc gattatccca agaaaggcgt tatcggtcag
      901 gatattgcag atcacccttg tggaagcaaa accgggaaac tgggtcaggc agtagagata
      961 gcccagcaca aagacgccga tggtgatcat cagcggcaag ttacgtttta tcatggctac
     1021 gtactccttt aatcagactg ataaagcgtt gcgactgaac aatcagcaca caaagcacca
     1081 ccaccgcttt caccacctgg ttcatctccg gcggaaagcc cgaaagcaaa attccggtgt
     1141 tcatcccctg aataatcagc gcccccacta ccgagagcag caggttaaag cgcccgccca
     1201 tcagcgatcc gccgccaatc accaccgcga gaatggcgtc cagctccagc cataatccgg
     1261 cgttgttggc atcggcaccg caaatatccg ccgccacgat aatgcccgca atcgccgcac
     1321 atagcccgct caacacgtag gtgagcatga cgatgattcg cgtgtttacc ccggcatttt
     1381 ttgccgcccg aatgttgata ccaacgactt cgataaacat ccccagcgcc gttttgcggg
     1441 tcaacagcca gaacaggaga agcgtcagca ccgcgataat gaccggcgtt ggcaggaaca
     1501 acagcgatcc gctgccaaac catgagagat ccggcgagtt aaacgtgacg atctgcccgg
     1561 cggtgatcag ttgcgccacg ccgcgcccgg cgaccatcag gatcagggtg gcgacaaacg
     1621 gctgaatttt gaggatcgct accagtatgc cgttccacaa tcccgccagg atgccggtgc
     1681 ccagggcgct taacaaaaca atcggcaggc tgaatcccgc gaccgtcatc gcagccgttg
     1741 tggctccggc gatagccatc accgccccca cagagagatc gataccaccg gtggcgatca
     1801 ccagcgtcat accaatcgcc agtaacgcaa cgggagctgc acggttaaga atgtcgatag
     1861 ggctaccgaa caaacgccca tcctgaagca ccacctgcca gaaatgcggg gctaccaggc
     1921 tatcgaccag cagcaccagc aacagtgccg ccagctgcgg catccccgtc ggccagtgaa
     1981 aacgcctttt tggcggcgta gtgtcaggga gagattgagg catcacactg ttctccttac
     2041 gccgcaatgg cgttcatgat cgccggaacg gaaagctgtg ccagcgggat ctccgccacc
     2101 tgtttgcgat cgcgcatgat gatcacccgg tcggcatagc ccaccagctc ttccagttcg
     2161 gaggagatca ccagcagcgc cagaccgtca gcacacagcg tttcaatcag gcggatgatc
     2221 tcggcgtggg caccaacatc aatgccacgg gttagctcat caaggatcag aaattgcggt
     2281 cgggtcagta gccaacgtga aagcagcact ttttgctgat tgccaccgga gagaaattca
     2341 atcggttgtt cagttgaagg tgtgcgaatg ccaagctggc ggataaagcg ttcggcaatc
     2401 tcttgctgct ctttgcggga aatgggtcgt agccagccgc gctgggcctg gagagcaagg
     2461 atgatatttt cccgcaccga ggcggcggcg atgatgccat cggttttcct gtcttccggg
     2521 cagaatccga tgcccagtac tgaagcctga tgtggcgatc gcagggtttg cggtttgcct
     2581 ttgatcaacg ccgtgccgct gtcagcaggt ttgataccga agatcacttc ggcggtttcg
     2641 gtacgtccgg atcccagcaa gccagccaga ccgacgatct cgccggggcg cacttcgaga
     2701 tcaaacggtg ctatcgttcc ttttttgccg taatttttga acgcggcaac gggtttgtcg
     2761 ctcaacaatg ttcgcccggc acgctgtagc gcgtgggtat ccagctcgcg ccccagcatc
     2821 atttttacca gttcgatctg cggtagctcg cgcgtttccc gacagcctac gaaactgccg
     2881 ttgcgtaaga cggtgatccg atcgctgacc tgatagacct gatcgagaaa gtgagtgaca
     2941 aagatcaggc tgacgccgcg atcgcgcaac tgacgcatca ggccaaataa tagctccact
     3001 tcctgggtgt cgaggctggc ggtgggttca tcgaggatca gcactttggc ggagagatcg
     3061 atagcccggc aaatggcgac gatttgctgc atcgccaccg aaaaacggtt aagcggttcg
     3121 cgcacgtcga gggagaaacc gtaagatgcc atcagttcgg tggcgcgctt ttccatctct
     3181 ttacggcgta gaaggccgaa acgtttgggt tcgcgaccta tgaatagatt atcagcaacc
     3241 gacatattgg gtagcaggtt gacttcctga tagacggtgc cgataccgag ttgttgtgca
     3301 tgggcggtat ttttcggtga gatagtctgg ccttccagcc agatggtgcc gcgatcggcg
     3361 tggtatacac cagttaatgc tttgattagc gttgattttc ccgccccgtt ttcaccaagc
     3421 agcgccatga tttcgccacg gcgcaggctg aaatcaacgt tgtctaacgc tttgacgccg
     3481 gggaaaaatt tacttaatcc ttcggtgcgg aggatctcct ggtgttggtc ggtcgtcatg
     3541 gtttcccccg gtgtaaagcg tgctcccagt aggcatgata agacgcggta agcgtcgcat
     3601 caggcttcag tgcaccatcg ccggatgcgg cgcgagcgcc ttatccggcc tacgaatcgg
     3661 catcaggcat agcaaccctc aataccccat atttttcttc ttctctaact cttcttttgc
     3721 ggtatcaggc aggtaaaggg tggatttggt taacgtcagc ttttcaggca tggtgccgtc
     3781 ttttttgtat ttctccagcg catcgaaagc gggtcccgcc atgttcggcg tcagttcaac
     3841 actggcgttc gcttcgccat ccatcatcgc tttgtagatg tccggtacac cgtcaatgga
     3901 acccgtgagg atatctttgc ccggtttcag gcccgcttct ttaattgcct gaattgcacc
     3961 aatcaccatg tcgtcgttat gggcgtaaac catgcagatg tttttgccgt tgttttccgc
     4021 tttgataaag ctctccatga cttctttgcc tttactgcgg gtgaagtcac ctgactgcga
     4081 gcggataatt ttgatatttg gcgcattctt aatggcttcg gcaaagcctt tcttaacggt
     4141 caatggcgac gctggcccca acggtgccct gcagctccac cacgttgcat ggtttgccat
     4201 tcacttcttt taccagccag tcaccgatca acttaccttc gaggatgttg tcggcagtaa
     4261 cggtggtcat atagagagat ttgtctttca catcgatgga acggtcgagc aagaagaccg
     4321 ggatttcggc atctttcgcc tcttttaata ccggttccca acccgtcgca accactggag
     4381 cgataaagat cgcatccacc ccttgtgcaa cgaaggaacg taccgcttta atctggtttt
     4441 cctgcttttg ctgaccatcg gcaattttca acgtaattcc gcgtttttcg gcctcacttt
     4501 tcgccacatt ggtttctgcg gcgcgccagc cggattccga tctgacctgc gaaaatccta
     4561 cggttaatgg agcggccaac gccatagacg acatggctgc cgagactgca gagactacaa
     4621 gtaagcgttt ccacataggg ttgtcctcgt cggggtaatt attattggtg aaaagatgtt
     4681 cgcgaaaaaa ctatagacaa ttcgttatgt aacggattgc gttacatcac acttcaaaaa
     4741 agtaaataaa acgttaatca caagtttgta atcgctttca tctcactatg aaaaatgcgg
     4801 ctacggttat ggattttcct gctctgtata ccgtcttaaa actggcgaaa aaggaaaatg
     4861 aagacgaaaa caagcaaaga cattcggcgc gagttggcta taatacttgg cacttgtttg
     4921 ccacatattt ttaaaggaaa cagacatgag cttactcaac gtccctgcgg gtaaagatct
     4981 gccggaagac atctacgttg ttattgagat cccggctaac gcagatccga tcaaatacga
     5041 aatcgacaaa gagagcggcg cactgttcgt tgaccgcttc atgtccaccg cgatgttcta
     5101 tccgtgcaac tacggttaca tcaaccacac cctgtctctg gacggtgacc cggttgacgt
     5161 actggtcccg actccgtacc cgctgcagcc tggttctgtg atccgttgcc gtccggttgg
     5221 cgttctgaaa atgaccgacg aagccggtga agatgcgaaa ctggttgcgg ttccgcacag
     5281 caagctgagc aaagaatacg atcacattaa agacgtaaac gatctgcctg aactgctgaa
     5341 agcgcaaatc gctcacttct tcgagcacta caaagacctc gaaaaaggca agtgggtgaa
     5401 agttgaaggt tgggaaaacg cagaagccgc taaagctgaa atcgttgctt ccttcgagcg
     5461 cgcaaagaat aaataagttc ttctagcgca ataaccctga acgccgggct tcggttagta
     5521 agggtttttt tatgcccgcg ataaataaac tctctattcc accatcatta ttctcagcgg
     5581 ttgcaaggct tgaacggtaa gaacaagcaa acccgaccac cattttgctg ttcatagcca
     5641 cttgctggaa gttagcagac ctcactcata ctcaccgttc aaagcgtgcc cccgttttac
     5701 ccctgcacat ttgtgaacgc cagcagagtt ttttatgcga ttgcagatat ggaaagcggc
     5761 tcggagagaa aagcaaaaga tggaaaagtt acaaaaaaaa atcgcgagat acatcgcata
     5821 aaacaacgcc acccgaagga ggcgtgcttt tattattact tatccctgtc taaccagtcg
     5881 ccgctttcaa ttagctttaa tccatcgacg ggtcgttgat aaacgtacat ccatgcactc
     5941 ccatacggcg tctgaatcaa ctggcgcgcg tattcaccgc ccctggtgcg caaggcatca
     6001 agttcggcca gcgtggcgtt gtcaatacga taaacttcac cgtgtaccgt tccgttcccc
     6061 ggaactgcgc ctggatagtg gcccaggcta tacaactggt agttatcgat actgaaatcg
     6121 cccagtaact gggcattggt catccagtga ctgttgcctt gtttgtggcg taaactgccg
     6181 tagacaaata ttcgcattgc taaaactcga actgatagag caaatccagt gcctggtcta
     6241 caccagacac ggcttccaga tatagcttag gcatcaggcg ataacgtaac gtgagtgttg
     6301 ctatagagtc aaatataccc acgccgtatt tcacttgcag acctggcaat acatagccgc
     6361 tgaccactac ctgggaggag tcgcctactc ccagggtgtc gagcgctaaa ttgcttacgc
     6421 caaacgtctc gccgatttta cccacaatct ggccactttg cgcaaccccc agaccaatca
     6481 gcatcgaggt cattgccgca ctgtcgctct gatcgctctc cagcccttgt ccacgtagca
     6541 aataagacaa tgcagcttgt tgcgacatcg ccgggtcaga gaagatctcc gctttcggtt
     6601 cgtccgccag accagtgacg cgaactccgg cgattacatc gtcttctgta gcatccgggt
     6661 tacgaatagc ttcaatatta agatacggtt gatccggcgg accagagaac agtaactcac
     6721 ccttacgcac aatcagatcc tgaccatagg catggaagcg cccttcaggg atgttgatct
     6781 gcccgttcag gcccagccct tgtttgtcct gtacgacgtt aagatcgccc gtcagccgcg
     6841 ctttcaggcc aaaggcgtca atgcgcacat tgttgccaac gtggacaatc aggttactgt
     6901 taatcggaat cgacgccgtt ttcggctctt ccggttgcag gttatcgtta agcatcacca
     6961 catcgctgga gacgcctact gcgctttccg gcagatcgtg caccacgatg cgcgcccacg
     7021 ggacatccac gcgaccatcg agggtaaaca ggtttggtgt agcctcgaat acaacatctg
     7081 gcgatacatc cattcgtacc atcggcggca cggtgatccg cactttactg cctttcgccg
     7141 ttactcgtgc ccgccagttt tcaatttggc tccagtcggc atcaccgttc aggtagattt
     7201 caccctgctg ggtccgtact gtaccggcaa gcgtcgagcg cataccgtta aagttgaccg
     7261 caagctggct cggctgcata tcaaacggca taaagttgcc gtcgatatcc acacccgtaa
     7321 cctgaagctg accaaacaac tgcgggcttt gcacatcacc acccagacgc aagttggcac
     7381 tcaccatccc cgctgctttt tccccacggg taaagatggg gtttatcatc gcaaggttga
     7441 agttacggat attgacgttg ccaccaagat tacggcggcc ttgcggatcg gtcacctgca
     7501 cctgtccatc aaactggccg ttattggtca ggcggatggt ccagcccaat tcggcacggt
     7561 tgttacgcaa ttccgccgtc agattcagtg tctgaaacgc caccggcagc gccgcatcgt
     7621 tgacggtttg cgttacctgc acgttacgcc ccgaaagggt gatactgccc tgcggcagcc
     7681 cctctttcgt ggtgtcccag gcaacatccg ctttacccgt gaagataccg ctggcctgag
     7741 tggtttctgg cataaacggt ttcagcatgg cgaggtcgaa gcggttgaga ttcaccaccg
     7801 cacgcccttc ggcccccgca tcgatagttt gcggcacgca cagttccgca ttcgggttaa
     7861 gccagcagtg tggcccgatg ctgatttttt gctccttatt gcggtaatcc agcgcaatat
     7921 cgcgggtcag cgaccacggg ccgaccggcg tctggaagcg ggtattgcta agagttccct
     7981 tccagcgttc ttctttgcga tcaaaacttc ctgccagatt aagctgcccg gagacaggct
     8041 cgccctgaat ccgcaactgt agctcgtgct gcttttcgct gcctttggca ttcagggtga
     8101 cgaggttgat atttacatcc ggctgcgaaa tttgctcaac gcgtacgtcg agtttcccgg
     8161 cgatctgatc ggtggattta atgtcgcctt ccacgcgaac ctgcgccacg gaaagttcct
     8221 gccagcgcag gccgcgcgcg gtgatatctg ccagtagttg tggcgcttcc accgtgccgc
     8281 gtactttcac cagccctttc gctgtaccgc caagccccgg cagcgcgtta tccagccccg
     8341 gcgcgttgat ggtggcatca agattgagat cttttacccc cagctcgcct ttcacttcgg
     8401 cactgtttgg cccgagttcc agatgaagcc cagggatcat ccactgcata taactgttgc
     8461 ctttcagcgt gccgtcaacg ttcactttgt tctgtttgac gttaccggtc agcttcagtt
     8521 ctggcacctc catctgccag gtgccgccgt acaggctacc gcgggtttta atcaagccat
     8581 tgagtttcga cggccactcc gggaactctt tggcggtgta aatgccatta agcgttagct
     8641 caccgcgcca actaatggcc tgctgccagt cgagcaacgc cttgagttca gttttccctt
     8701 ccagcgccgc gacggtgagt ttgtcgagat tgacctgctg ttcattacct ttggcatcaa
     8761 gggtaatggt ggctggcggg atctcctgtc ctttcactgc cgtacgcata gagagcgtgt
     8821 aatcggtcat tttgccggta agtttcagtt tcagatcatc cgcctgatac tgcttctcac
     8881 cagtgaacgg ccagtaaagc tgtttgctgt tcacttccac gttgagtggc aatccggctt
     8941 ccgccagtcg cgtctgggcg cgtaaatcca tatccaccgg accggaaagg ttaacgccta
     9001 tctccagctg ttcgcgcagc gcgccgccca ctttcagctt caccttttca cctttcaacg
     9061 gctccacgtt cagtgtgctg ttgagggtga tatccaccgg ccagttgtct gacagctgcg
     9121 ccgtgccgct ggcgttgacg atcccctggt tggaatcgat atccaaggcg tccagtttag
     9181 tattgccgtc aatgctgctc actttcagca gcatggtgcg cacggtgatg tccgtgtcgc
     9241 ccgtcacgcg cagctgctcg cccttaaact cttcaatgtt caggttaagc ggcaaatgca
     9301 cgtcggtcat ttccggcaat accgggcgag aaaagagatc tttcagcgtt tcgccgagcg
     9361 gcttttcctc cggctgcgga ttttcaattt tcggttcgac cacttcttcc tgcgccactt
     9421 ccaccacttt cggcagagca atcagcaggc ctttcagcga cgtcggtttc agggtcaggg
     9481 ttttctcctg ccagttcagg ccggaggtga agtccatcac cgacaccgtg gtgtcatcaa
     9541 tcttgatgtt gacgttgtcc agcgccaccc gtgtcagggt gatgggatac ggcgtggaga
     9601 gatccagcgg accgctatct tcctcttctt caacctgttc agaaggaggc atttttttac
     9661 tgtcgatgtt gacctgaatg tctttcagcg ccaggtcatt aatacaaacg ctactgttcc
     9721 acaggcactc aagaccgacc gccagatgca gattacccgc ttttaccgcc acgcctagct
     9781 gctcataacg aacgtcagac aaggtgagat cgcgccagcc gccggtgacc ttgccaatat
     9841 ccagtcctgg cacccagcga tccgccgctt taaatacaag atgcaggccg ctggtggtgc
     9901 ccaccagaaa cgccaccgat cccagcaaca gtaagataac gataaccacg ccgaggctga
     9961 tttttttcca taaactcata attctggccc cagaccgatg taaaactgta acccgtgttc
    10021 gtctttatcc gcgaccggta cggcaaaatc gagtttgatt ggcccgaccg gcgattccca
    10081 gcgtacgccg accccggtac cggttttaaa gtcgctgcgg cgaatatcgc ttaccgcttc
    10141 gccactatcg acaaacaccg cgccccacca ttttccggtc acgttgtact ggtattccag
    10201 cgatccggtt atcaacttcg aggccccttt caggtcaccg ttggcgtatt tcggggcgat
    10261 agatttgtat ttgtagccgc gaatactgcg atcgcccccg acgaagaaac gcagatccgg
    10321 cggtactttg tcgaaatcac cggtttcaat ccagcccagc gtgccgcgtg tcacaaagcg
    10381 atggcgatcg tacagtgtgc ggatccagac gttctgcgcc tggaaaacgg agaaatcgac
    10441 atctgaaccc caggccgtgt tggagtagtc gatagagtag cgttgcgagt ctccccaggt
    10501 tggcatcagg ccaccacgag aacgcgtgcg gctaatcatc accccaggat aaaacagcat
    10561 cgtggtgttg gtaatttcac cctgggtaaa gtggtcgagg ctccagcgca ggttaatggc
    10621 acgctgccag ccgctggaga gatcccagta gcgagaagcc accagcgtag tggagtcaga
    10681 ttcggtatcg ttcaggtcag tgcgcttaaa accgccctgc accaaataat attgttccag
    10741 tggattcttc agcagcggca ttttatagct gaagtcgagg gtctgttccg gcgcggaaat
    10801 actggtactg gtggtcaaac tgtgaccgta tgagttcatc cacggctttt tccacgtcgc
    10861 tttcacgcgc ggtcccacgt ccgtagagta accgcccccg gtttcgatgg tgttttctgt
    10921 tcgcggcgaa accacgcccg tcaatggtaa tactttcgtt tcgcgcgctt tatcaaattg
    10981 tggagctacc accaccgagt taaaccagcc ggtagcagaa agtcggcggt tcagttccgc
    11041 caaatctttc gattcatact catcgccctc tttaaacggc accagatttt gcaggtattc
    11101 atcgcggatt tgtgatcctt caaaggtcac atgcccaaag cggtaacgtt cgccactgtt
    11161 ataatcaata tcccagaagg ctttatgcag gccgagcgca atgcccagct gcgctttggt
    11221 aaattcgcta tcgaaataac ctttacgcaa cgcaatactg gttaaggact ttttgaaatt
    11281 ttcataatcg ccctggttca gtaccgtacc aatagccggg cgagtatcga gcaatttcaa
    11341 atagtcttta tcggtccgcg cgccgccgcg caataccaca tcggtgccgc caattaacac
    11401 cggcacacct ggcgtgactt tggcgatcaa tacctgccgc cctttctttg gcggtggacg
    11461 gagatcaaat tcaatggtcg gctggtaata acccagcgct ttcagacctt cacggatggc
    11521 atcatcgacg cgtgcgcgaa agcgacggtc tggcgtcact tcatcacttt caatcgtaga
    11581 aagctgcgca cgaacgttct tttccagctg tcccgataac ccctcgacct gtagacggac
    11641 gttcgcggcg acggcagatc cgcttaagca gagtaagctt acacagcata actgtcggat
    11701 atagcgcaca ttttctcctg aatatccttt tttcctgccc ctggaacgcc gttaaacggc
    11761 ttaacaaaaa tccagtaata tggattaaaa agcagactaa accccaaata tttcttatgt
    11821 tttactttag acctattcac ggtggttatt gtgtgcaaat acgcctcttg ttacaacctt
    11881 aaccccaatg accgattttc gggagagcga caccatgagt ttatttgata aaaagcatct
    11941 ggtttccccc gccgatgccc tgcctggacg taacaccccg atgcccgtag ccacgctgca
    12001 tgcggtcaac ggtcactcaa tgaccaatgt acctgacgga atggagattg ccatttttgc
    12061 gatgggttgt ttctggggtg tggagcgtct gttctggcag ttacccggcc tttacagcac
    12121 cgccgcaggc tataccggcg gctatacgcc aaatccgact tatcgggaag tgtgctccgg
    12181 tgatacgggt catgccgaag cggtacgcat tgtttacgat ccttccgtca tcagctatga
    12241 gcagttgcta caggtatttt gggagaatca cgatcccgcc cagggaatgc gtcagggcaa
    12301 tgaccacggc acgcagtatc gttcagcgat ttatccgctg accccagaac aggatgccgc
    12361 agctcgcgcc agtctggaac gttttcaggc ggcgatgctt gccgccgatg acgatcgtca
    12421 catcaccacg gaaatcgcta acgccacacc gttttattat gccgaagatg accaccagca
    12481 atatctgcat aaaaacccat atggttactg tggaattggc ggaattggcg tctgtctgcc
    12541 gccggaagca tagcgttacg ggtacaaatg tagtttgttg ataaagtgct ctttgtctat
    12601 gcctgatgcg gcttaaacga cttaccaggc ctacaaaatc gtccaaattc aatatattgc
    12661 aaggactgcg taggcctgat cggcatagcg catcaggcag tattgcattt atcagcagtc
    12721 tgaattttaa ccctctggcg actttacagt accttacgct atactagcga ctgaaaatgc
    12781 cggttcactt tcttcgaatc ggctttcaat gtgtatttca cacaaattaa tcaactttcc
    12841 cttccgagga tctggcctga aaggtcggat aagatatgtt aaacagtatt ttagtcatac
    12901 tctgcttgat cgctgtaagt gcgttcttct cgatgtccga gatctcgctt gccgcctcac
    12961 gcaaaatcaa acttaaactg ctggctgatg aaggcaatat aaatgcccaa cgcgttctga
    13021 atatgcagga aaatcccggc atgttcttta ccgtggtcca aatcggtctg aacgcagtgg
    13081 cgattctcgg cggtatcgtc ggtgatgcgg cattttctcc agcttttcac agcctgttct
    13141 cccgctatat gtcggcagag ctctctgagc aactgagctt tattctctct ttctcgttag
    13201 tgactggcat gtttatcctg tttgcggatt taaccccgaa acgcatcggt atgattgcgc
    13261 cagaagcggt ggctttgcgt atcatcaatc cgatgcgctt ctgcctgtac gtttgcaccc
    13321 cgctggtgtg gttcttcaac ggcctggcga acatgatctt ccgtattttc aaactgccaa
    13381 tggtacgtaa agatgacatc acttctgatg acatctacgc ggtagtggaa gccggtgcgc
    13441 tggcgggcgt gttacgtaaa caggaacacg agctgattga aaacgtcttt gagctggaat
    13501 cccgtaccgt tccgtcttca atgacaccgc gtgaaaacgt gatttggttt gatctccacg
    13561 aagatgagca aagcctgaag aataaggtgg cggaacatcc gcactctaag ttcctcgtct
    13621 gtaatgaaga tattgaccac atcatcggtt atgtcgattc taaagacctg ctgaaccgcg
    13681 tgctggctaa ccaaagcctg gcactgaaca gcggcgtaca aattcgcaac acgctgattg
    13741 tgccggatac gttaaccctt tcagaggcgt tggaaagttt taaaaccgca ggtgaagact
    13801 tcgcggtgat catgaacgag tacgcgctgg tggtggggat catcaccctc aacgacgtga
    13861 tgaccacgct gatgggcgat ctggtcggtc aggggctgga agagcagatt gtcgcccgtg
    13921 atgagaactc atggctgatt gacggcggca ccccaattga cgacgtcatg cgcgtgctgg
    13981 atattgacga gttcccgcag tcgggcaact acgaaaccat cggcggcttt atgatgttta
    14041 tgctgcgtaa gatcccgaaa cgcaccgatt cggtgaaatt cgccggctac aaatttgaag
    14101 tggtggatat cgataactac cgcatcgacc agctgctggt gacccggatc gacagcaagg
    14161 ccaccgccct ttcgccaaaa ctgcctgacg ctaaagataa agaagaaagc gtcgcgtaac
    14221 ccccagaaac atcaacggct cctgaatcag gagccgtctt attactgcat agcactttgg
    14281 ttaagccatc tctgtttgca gacgcataac ctgacggtta acttcggaca tcactgacaa
    14341 atgcatctta tccttcactt ttgggataag aatcttaccc ttatcaaatt caaaagcgcc
    14401 aacgtccttg atgtataacc gtccacggaa caggatcttt acgtacttcg ccacctgaag
    14461 tgggttgtac cgttggaaaa ttttcattgt tatctcctgc tgagtattac gcctgtgcgg
    14521 tgccacaatc ggcccaacta ttatgaggcg caaattttaa tgcttagtga ctatagacta
    14581 tccgggcaat gtttccaccg cgtataactt tttttaccta aaggttacaa ttattcagaa
    14641 ttatcttttt accgaagcgc ggtcttcagt ataagcattc atttttcata tgatttgtgc
    14701 gcttgaccgc aaactggcat cacacttgcg ggaaattcga taaatagcac atatgattaa
    14761 aactcagacc caagtggtcg gatcacctgc atatcataag aaggaaacac catgacccta
    14821 cgcaagattc tggcactcac ctgcctgctg ttgccgatga tggcttccgc acatcagttc
    14881 gaaatcggtc agcgagtgcc gccgattggc attaccgatc ggggcgagtt ggtgcttgat
    14941 aaagatcagt ttagctacaa aacctggaac agcgcgcagt tagtgggaaa agtgcgagta
    15001 ctgcaacata ttgctggtcg cacctctgca aaagagaaaa acgcgacgct gattgaagcg
    15061 attaaatcag cgaagttacc gcacgatcgt taccagacca ccaccattgt taacaccgac
    15121 gacgcgattc cgggttccgg catgtttgtg cgcagcagtc tggagagtaa taaaaagctt
    15181 tatccctggt cgcagtttat tgtcgatagc aatggcgtcg cgcgcggtgc ctggcagctg
    15241 gatgaagaaa gttccgctgt agtggtgctg gataaagacg gtcgcgtgca atgggccaaa
    15301 gacggggcgc tcacaccgga agaggtgcag caagtgatgg acctgctgca aaagttgctc
    15361 aaataatggc aataacataa attctggaaa aataattttc ccctatcgtt atctatggga
    15421 taggggaatt aagtctctga gttcttacac cgaactaata ctggtaacgc tagttgctct
    15481 tttttaaacg cctcaatgat cgatttatat ttctcgcaaa tcttttcttt ggtaatatta
    15541 ctatctgcgg ccagattcgc acttactatc gcatccactt gttcggctaa ttcgacttgt
    15601 tggcaacctg cattaaccag aaaattataa ataagagagt aatcttcatc ctccaaaaca
    15661 acagaggaac cattacgaaa acgttcatcc cctccataga ttttaagcgc aaagcccaaa
    15721 gattctatga ctatggcaaa attatcatca attatttttt cgtaaatata ccatccatcg
    15781 cattctttaa ttccaccgcc tggtatttgg agttcaaatt taccccgttt taatttgctt
    15841 actacatcct ccaataaggc ataaccgaaa gatacgtcac caaacgcctg gcgaatataa
    15901 cctttaatcc tttttggtat gtcagactgt tttttctgca aaatatcaca ggcat
//
LOCUS       DAETPZ010000087        15127 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.087, whole genome shotgun sequence.
ACCESSION   DAETPZ010000087 DAETPZ010000000
VERSION     DAETPZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15127
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.087"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            596..793
                     /locus_tag="K0W59_003142"
     CDS             596..793
                     /locus_tag="K0W59_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024078.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(743..1936)
                     /locus_tag="K0W59_003143"
     CDS             complement(743..1936)
                     /locus_tag="K0W59_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3024079.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2072..3796
                     /gene="iucA"
                     /locus_tag="K0W59_003144"
     CDS             2072..3796
                     /gene="iucA"
                     /locus_tag="K0W59_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HBI3024080.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3797..4744
                     /gene="iucB"
                     /locus_tag="K0W59_003145"
     CDS             3797..4744
                     /gene="iucB"
                     /locus_tag="K0W59_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HBI3024081.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4744..6486
                     /gene="iucC"
                     /locus_tag="K0W59_003146"
     CDS             4744..6486
                     /gene="iucC"
                     /locus_tag="K0W59_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HBI3024082.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAEVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6483..7799
                     /gene="iucD"
                     /locus_tag="K0W59_003147"
     CDS             6483..7799
                     /gene="iucD"
                     /locus_tag="K0W59_003147"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HBI3024083.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSPASLTHYTANIQE"
     gene            7805..10000
                     /gene="iutA"
                     /locus_tag="K0W59_003148"
     CDS             7805..10000
                     /gene="iutA"
                     /locus_tag="K0W59_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HBI3024084.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10551..10694)
                     /locus_tag="K0W59_003149"
     CDS             complement(10551..10694)
                     /locus_tag="K0W59_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024085.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10955..14842
                     /gene="sat"
                     /locus_tag="K0W59_003150"
     CDS             10955..14842
                     /gene="sat"
                     /locus_tag="K0W59_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HBI3024086.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPEETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGN
                     GKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEA
                     VLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTM
                     QGHATEHAIFRSRTSHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQP
                     DWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNN
                     GFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAK
                     VIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMA
                     SVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSM
                     NNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQADQ
                     LVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTP
                     VIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDI
                     NGESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGS
                     DAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYA
                     GAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVD
                     VGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAE
                     IRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
     gene            complement(14982..>15127)
                     /locus_tag="K0W59_003151"
     CDS             complement(14982..>15127)
                     /locus_tag="K0W59_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI3024087.1"
                     /translation="YRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQ
                     MAA"
BASE COUNT         4074 a         3594 c         3752 g         3707 t
ORIGIN      
        1 gagtgaaaca aaggcagctt atcgttgttt ttcaaacgat aaagtctctg ctaataaaat
       61 catggctcct cacaaagaaa atatcactga aagagctcag cagtttaaaa gagttttagt
      121 tttacaggat acaacagaac ttaattattc tggtcagaaa gaaaaacaag gagttggtcc
      181 caaaaaacat aaagatgaaa gaaacttatt tcttcatcct caactcgtca tcagtgagtc
      241 aggtgtttgc ctcggtgttt atgatgacta tcagtggttc agggatgaat taaaaaccag
      301 taaaaatacc cgacaagaaa tctgcaatga tttacttcat aaaaaacatg tcagtgaaaa
      361 agaaacctgg cgttgggttg aaggatataa taaagcaaca gaactggctc gccaatgccc
      421 tgatacccat gtattgtcaa tttctgatcg tgaaggcgat ttttatgacc tgtttgaacg
      481 cgcagcgcag actcccggaa taaaagctga ctggcttgtc agaatgaaat ttaataacag
      541 agcaacgctg aatattaatg gtaaacggga tcataggttg ttgcacgaaa gaatcatgga
      601 gattacaccg cagcaactgg ttgaattcac tatacctgat gggcgggggc agcaagccag
      661 aacactgcct gacatgcttt catcagacgc aggtcctctc tcatatcctc tgatttactc
      721 cagtaaggtg atgatcatat ctttatttga tagtacggta atgccagata aatacagaca
      781 caatggtaaa taatatagtt gcaaataatg ctgccataat cagcgcgatt gcgacacctg
      841 ttttcgatac cagcggcacc agcaaagcgc acagtccata gccaagtgtg tgactggtag
      901 cgagccagcc cgcgcctgcg ccatcagcca gcttatcatt cagtagaagt tgataagcgg
      961 gtgttgccag agctgctcca aatgacagca ctgcacagcc cgggtaaaac aaccatagct
     1021 gttcggagag catgatagcc agaccaccac tcatcaacac tccggcactc aggagcaggg
     1081 ccaccggagt caggcgctgc ggacggagta ccccgaactg cgcgataagc gcagctactg
     1141 cggacagacc caacagccag gccacctgtt ggctaatggc ggtggtatcc gtagcgaact
     1201 ggcgagtaag ggcaggcgaa agtccaagct gcatcatgct caccgccgca gccagtaata
     1261 ttgcgcaaag cagataaggc agacaatccc gcttcagact gacgctcttg cactccggtg
     1321 tgggctgtgg tggtgtgccg ggcaaccgca gcagcatcag cagcgccagc actggggctg
     1381 ccatcagcag ccccagtggc gccagaggat gaatggccag cattgctgcc gcgcacagcg
     1441 gcccgaatag cctgccgcaa ctcaggccgg agctgatggt tgccagagcg gccatgcgat
     1501 tcccttctcc agctctctgc aatgcccaga cctgacaggc tggcaccatt gccgacacgg
     1561 tcagcccgta ggcgatccgg gcgatgatta atattcccag tcccaccgct gttgttatgg
     1621 cgctggtagc catcagcacg cttcccagcc ctagcagggt aaagcttatg ccatatcctc
     1681 ccagagccca caacaccaca ggtttactgc cagtacggga aatttgcttt ccccaccacg
     1741 gggacgatgg cagaaacagc attgagccaa gcatcagcag ggcagcccat acagacagac
     1801 tcagatttgt ctgtatgacc agaacaggga gcacaaccag cagaccgttc tgtccgatac
     1861 cgagaagccc ggcactgaac gcaagtggcc agcaggacag tggtttttgg ggcgtatctt
     1921 cgatccctgg tgacattata atgtttcaac tccatgtatt aattgtgttt atttgtaaaa
     1981 ttaatttatc tgacaataac atttattgtt gataatgaga atcattattg acataattgt
     2041 tattatttta ctgtgtggga gctgtttgac tatgaccctg ccctctgaaa aaccagccac
     2101 agatgtggct gcgcagtgct tcctgaatgc actgattcgt gaaaccacag actggaaact
     2161 gacagaatac ccgccagacg aattgctcat cccgctggat gagcagaaat cgctccattt
     2221 cagagtggct tatttctccc caacccagca tcaccgcttt gcatttcctg cacgtctggt
     2281 cacggcatca ggcagttatc ctgtcgactt taccactctc tcccggctta ttattgataa
     2341 gctacgccat caactgtttc tgcccgttcc cctctgcgaa actttccacc agcgcgtgct
     2401 ggaaagccat gtccatacgc aacaggcaat tgatgcccgt catgactggg ccgctctgcg
     2461 tgaaaaagcg ttgaattttg gcgaggctga gcaggcgctg ctgacaggac acgctttcca
     2521 ccctgcgcct aagtctcatg aaccgtttaa ccggcgggag gctgaacgct acctgcctga
     2581 tatggcaccc cacttcccac tgcggtggtt ttcggtggat aaaacgcaaa tcgccggtga
     2641 aagtttgcat ctcaaccttc aacagcggct gacgcgattt gccgcagaga atgcgcctca
     2701 gttactcaac gaattaagtg acaaccaatg gctgttcccg ttgcacccgt ggcaggggga
     2761 atatcttttg cagcaggggt ggtgccaggc acttgttgct aaagggctga ttaaagactt
     2821 aggtgaggcc ggcacgtcgt ggctgccgac cacctcttcc cgttccctct actgtgccac
     2881 cagccgcgat atgatcaagt tctccctgag cgtacggctg accaactcca tccgtactct
     2941 gtccgtgaaa gaagtgaagc gtgggatgcg cctggcacgc ctggctcaaa ccgacggctg
     3001 gcagatgcta caggtccgct tcccgacttt ccgggtaatg caggaggatg gctgggccgg
     3061 gctgctcgat cttaacggca acatcatgca ggaaagtctg tttgccctgc gtgaaaatct
     3121 gctggtggat cagccgaaaa gccagactaa cgtactggtc tccctgactc aggccgcacc
     3181 tgatggcggt gattcgctgc tggtttcggc ggtaaaacgc ctgagcgatc gcctcggtat
     3241 cactgtgcaa caggccgccc atgcatgggt cgatgcatac tgtcagcaag tgctaaagcc
     3301 gctgtttacg gctgaagcgg attacggcct ggtgctgctg gcgcatcagc aaaatattct
     3361 tgtccagatg cttggggatc tgccggtcgg atttatttac cgtgactgtc agggcagcgc
     3421 ttttatgcct cacgcgacag actggctcga ttctattggc gaggcgcagg cggaaaatat
     3481 tttcacccat gagcagttgc tgcgctattt cccttattac ctgctggtta actccacttt
     3541 tgctgtgacc gctgcgctgg gggctgccgg gctggacagc gaatcgaatc tgatggctcg
     3601 tgtacgagca tcgctggctg aagtgcgtga tcaggtgact cataaaacct gtctcaacta
     3661 cgtgctggaa agtccgtact ggaacgtaaa aggtaacttt ttctgttatc tgaacgatca
     3721 taacgagaac accattgttg acccttcggt gatctatttc gatttcgcta acccgctgca
     3781 ggctcaggag gtctgaatgt ctgaggcaaa cattattcac agcagatatg gactgcgctg
     3841 tgaaaaactc gacaagcccc tgaatcttgg ctggggactg gacaatagcg cggtgttgca
     3901 ctgtcccggg gagctgccga cagggtggct gtgcgatgcg ctggatcaga tatttatcgc
     3961 cgcaccacaa ctgtcagcag tggctctccc ctgggccgaa tggcgtgagg agccacaggc
     4021 gctgacgctt tttgggcagg taaaaagcga cattatccat cgtactgcct tctggcagtt
     4081 accgttatgg ttgagttcac cggcaaaccg ggcctccggt gaaatggttt ttgatgcaga
     4141 gcgtgagatt tatttcccgc agcgcccacc ccgtccacag ggtgaggttt accgccgtta
     4201 cgatccacgt attcgcagga tgctgagttt ccgcattgcc gatcccgttt ctgatgcaga
     4261 acgtttcacc cgctggatga atgatccgcg cgttgagtat ttctgggagc aaagtggctc
     4321 actggaggta cagaccgcct atctggagcg ccagctcacc ggtaaacatg cattcccgct
     4381 gattgggtgc ttcgacgatc ggccattcag ctatttcgaa atctactggg cggcggaaga
     4441 ccgcattggt cgtcactatt cgtggcagcc ctttgaccgt ggcctgcatc tgctggttgg
     4501 tgaacagcaa tggcgcgggg cccactatgt gcaaagctgg ctgcgcgggc tgacacatta
     4561 cctgctgctg gatgagcccc gtacgcagcg cacagtactg gagccacgca ccgataacca
     4621 gcgcctgttc cgccatcttg agcctgcggg ataccggaca attaaagagt tcgacttccc
     4681 acataagcgc tcgcgcatgg tgatggcgga tcgtcatcac ttcttcacgg aggtcggtct
     4741 gtaatgaatc acaaggactg ggattttgtt aaccgtcggc tggtggcaaa aatgttgtct
     4801 gagatggagt atgagcaggt tttccacgcg gaatctcagg gcgatgacca ctactgcatt
     4861 aacctgccgg gagcacaatg gcgcttcatc gctgaacgtg gtatctgggg ctggctgtgg
     4921 attgatgccc aaacgttgcg ctgcacggac gaaccagtac tggcgcagac gctgctgatg
     4981 cagctaaagc cggtgctgtc aatgagtgat gccactgttg cagagcatat gcaggatttg
     5041 tatgccacgc tactgggcga cctgcaactg ctgaaggccc gtcgcggact gagtgccagt
     5101 gacctgatta atcttgatgc cgatcgtctg caatgcctgc tgagcggtca tcctaaattc
     5161 gtttttaata aaggtcgccg tggctggggc aaagaggcgc tggaacgata tgcgccagag
     5221 tataccaaca ccttcagact gcactggctg gcggtaaaac gtgaacatat gatctggcgc
     5281 tgcgataacg atctggatat tcagcagttg ttgactgccg caatggatcc gcaggagttt
     5341 acacgcttca gtcaggtctg gcaggaaaac gggctggatc acaactggct gccgctgccg
     5401 gtacatccgt ggcagtggca gcaaaaaatc gccacagact tcatcgctga ttttgccgaa
     5461 ggcaggatgg tgtctctcgg cgagtttggc gaccagtggc tcgcccagca gtcgctgcgt
     5521 accctgacca acgccagccg ccggggaggg ctggatatca agctgccgct gaccatctac
     5581 aatacctcat gctaccgggg gatccctggc agatatatcg ctgccggacc actggcttca
     5641 cgctggctgc aacaggtttt tgcgaccgat gccaccctgg tgcaaagcgg cgcagtgatc
     5701 cttggtgaac cggctgcagg ctatgtgtcc catgaaggct atgccgcgct tgcccgggct
     5761 ccctatcgct accaggaaat gcttggtgtt atctggcggg agaatccgtg ccgctggctg
     5821 aaaccggatg aaagtcctgt tctgatggca acactgatgg agtgcgacga aaacaatcag
     5881 ccgctggcag gcgcatatat cgaccgctcc ggactggacg ctgaaacctg gcttacgcaa
     5941 ttgttccggg tggttgtggt tccgctgtat cacctgcttt gccgctacgg tgtcgcgctt
     6001 attgcacacg gacaaaatat aacgctcgcc atgaaagagg gggtaccaca gcgtgttctg
     6061 ctgaaagact tccagggcga tatgcggctg gtgaaagaag cgtttcccga aatggactct
     6121 ttgcctcagg aggtgcgtga tgtcacatcc cgtctgagtg cggactactt aatccatgat
     6181 ttgcagacgg gtcacttcgt gacagtactg cgttttattt cgccactgat ggttcgtctt
     6241 ggcgtacctg aaaggcgatt ttatcaactg ctggcagaag tgttgagtga ttacatgaac
     6301 aaacatccac aaatggcaga gcgttttgcg cttttctcac tcttcaggcc acaaatcatt
     6361 cgcgtggtgc tgaacccggt aaaactgacc tggccggacc tggatggcgg cagtcgcatg
     6421 ctgccgaatt accttgagaa tctgcaaaat ccgctgtggc tggtaactca ggaatatgaa
     6481 tcatgaaaaa aagtgtcgat tttattggtg tcgggacagg gccatttaat ctcagcattg
     6541 ctgcattgtc acatcagatc gaagaactga actgtctctt ctttgacgaa catcctcatt
     6601 tttcctggca tccgggtatg ctggtaccgg attgtcatat gcagaccgtc tttctgaaag
     6661 atctggtcag tgcagttgca cctacaaatc cctacagctt tgttaactat ctggtgaagc
     6721 acaaaaagtt ctatcgcttc cttacaagca ggctacgtac agtatcccgt gaagagtttt
     6781 ctgactatct ccgctgggct gctgaagata tgaataacct gtatttcagt cataccgttg
     6841 aaaatattga tttcgacaaa aaaagtcgat tgtttctggt tcagaccagt cggggagaat
     6901 attttgccca caatatctgc cttggtacag gaaaacaacc ttatttacca ccctgtgtga
     6961 agcatgtgac acaatcctgt ttccatgcca gtgaaatgaa tcttcgtcgg ccggacctga
     7021 gtggaaaacg gataaccgtg gttggtggag gacagagtgg tgcagacctg ttccttaatg
     7081 cattacgcgg ggaatgggga gaagcggcgg aaataaactg ggtctcacgg cgtaataatt
     7141 ttaacgcact ggatgaggct gcttttgctg atgagtattt tacacctgaa tatatttcag
     7201 gcttctccgg actgaaggaa gatattcgcc atcagttact ggatgagcag aaaatgacat
     7261 cggatggcat cactgccgat tctttactga ccatttatcg tgagttgtac caccgttttg
     7321 aagttctgag aaaaccaaga aatatccgtc tgctacccag tcgctcggta acaactctgg
     7381 aaagtagtgg tccgggctgg aggttattga tggaacatca tctggatcag ggcagagaga
     7441 gcctggaaag tgatgtggtg attttcgcca caggttaccg ttctgcattg ccacaaatac
     7501 ttccctcact gatgcccttg atcaccatgc acgataagaa cacctttaaa gtgcgtgatg
     7561 acttcactct ggaatggagt ggcccgaaag aaaataacat ctttgcggtc aacgccagca
     7621 tgcaaactca tggcatcgcc gaaccccagc tcagcctgat ggcctggcga tctgcacgta
     7681 ttcttaatcg cgtactgggg cgtgatttat tcgatctcag tatgccgccc gcactgattc
     7741 agtggcgcag cggcagccct gcttctttaa ctcactatac agcaaatatt caggaataac
     7801 gatgatgcgc aaaaagtata tgccccgggc tcttggtccg ctgcttcttg tcgtgctgtc
     7861 accagctgtc gcccagcaaa acgatgataa tgagatcata gtgtctgcca gccgcagcaa
     7921 tcgaactgta gcggagatgg cgcaaaccac ctgggttatc gaaaatgccg aactggagca
     7981 gcagattcag ggcggtaaag agctgaaaga cgcactggct cagttaatcc ccggccttga
     8041 tgtcagcagc caaagtcgaa ccaactacgg tatgaacatg cgtggccgcc cgctggttgt
     8101 cctgattgac ggtgtgcgcc tcaactcttc acgttccgac agccgacaac tggactctgt
     8161 cgatcctttt aatatcgacc atattgaagt gatctccggc gcgacggccc tgtacggtgg
     8221 cgggagtacc ggagggttga tcaacatcgt gaccaaaaaa ggtcagccgg aaaccatgat
     8281 ggagtttgag gctggcacaa aaagtggctt taacagcagt aaagatcacg atgagcgcat
     8341 tgccggtgct gtctccggcg gaaatgacca tatctccgga cgtctttccg tggcatatca
     8401 gaaatttggc ggctggtttg acggtaacgg cgatgccacc ctgcttgata acacccagac
     8461 cggcctgcag cactctaatc ggctggacat catgggaacc ggtacgctga acatcgatga
     8521 atcccggcag cttcaactga taacgcagta ctataaaagt cagggagacg acaattacgg
     8581 gcttaatctc gggaaaggct tttccgccat cagcgggagc agcacaccat acgtcagtaa
     8641 ggggctgaat tctgaccgca ttcccggcac tgagcggcat ttgatcagcc tgcagtactc
     8701 tgacagtgat ttcctgggac aggaactggt cggtcaggtt tactaccgcg atgagtcgtt
     8761 gcggtactac ccgttcccga cggtaaatgc gaataaacag gcgacggctt tctcctcgtc
     8821 acagcaggat accgaccagt acggcatgaa actgactctg aacagccaac tgatggacgg
     8881 ctggcaaatc acctgggggc tggatgctga gcatgagcgc tttacctcca accagatgtt
     8941 cttcgatctg gctcaggcaa gtgcttccgg agggctgaac aaccataaga tttacaccac
     9001 cgggcgctat ccgtcatatg acatcaccaa tctggcggcc ttcctgcaat ccagctatga
     9061 cattaatgat atttttaccg ttagcggtgg cgtacgctat cagtatactg agaacagggt
     9121 agatgatttc atcgactaca cgcagcaaca gaagattgct gccgggaagg cgatatctgc
     9181 cgacgccatt cctggtggtt cggtagatta cgataacttt ctgttcaatg ctggtctgct
     9241 gatgcacatc accgaacgtc agcaggcatg gttcaatttt tcccaggggg tggcattgcc
     9301 ggatccgggg aaatattatg gtcgcggcat ctatggtgca gcagtgaacg gccatcttcc
     9361 cctgacaaag agcgtgaacg tcagcgacag taagctggaa ggcgtgaaag tcgattctta
     9421 tgaactgggc tggcgcttta tcggtgacaa cctgcggact caaatcgcgg catattactc
     9481 gctttccaat aagagcgtgg aaaggaataa agatctgacc atcagtgtga aggacgacag
     9541 gcgccgtatt tacggcgtgg aaggtgcggt ggactacctg atcccggata ctgactggag
     9601 taccggtgtg aacttcaatg tgctgaaaac cgagtcgaaa gtgaacggtc aatggcaaaa
     9661 atatgacgtg aaggaatcaa gtccatcgaa agcgacagct tacattaact gggcgccgga
     9721 accgtggagt ctgcgtgtac agagcaccac ttctttcgac gtaagcgatg cagagggtaa
     9781 cgatattaat ggttacacta ccgtcgattt tatcagtagt tggcagcttc cggtgggaac
     9841 actcagcttc agcgttgaga acctcttcga ccgtgactat accactgtct ggggacagcg
     9901 tgcacctctg tactacagcc cgggttacgg ccctgcttca ctgtacgact acaaaggccg
     9961 gggccgaacc tttggtctga actactcagt gctgttctga cggggatgct tttgcaacaa
    10021 gggtatgctg taaacaacat ggtccttggt ggccatgttg tttacagcca gtataatata
    10081 cagccttaac aagatattcc gtctccggaa tgggctgttc accactgaat aacattatgt
    10141 ctgttttatg gatctttgac tgaaatttat cgcatatcct catttcattg tccgtctgaa
    10201 aactaacctt acaagtaagc gtcgtctcag caccgtctgg cagatcctga aattcctgag
    10261 aggatgatct gcgcccaata tcctgagccg gaactttgct gcaatgaagg ccaacgaaaa
    10321 atgggggagt atgttataga gtacttgcta cagggtaaaa agaggtacct gtcgctggtt
    10381 ctccgttcag tttaaccggg agattttctc ctgcgcctgt cataaagtcc ggtaatggag
    10441 attgttaata cgatggtgca cgatgcatcg tcagtgcccg gcccggagga tgccccgtag
    10501 ctacatacgg atcagggttg gcaatatcga atggcaggct ataggcaaag ttaaagcccc
    10561 atggagtagc acaaaatatg ccgcacaaag gaaacggtct gaataacgca gtgatggaga
    10621 acttcttcgg cacactgcaa aatcgcatcg tggtcgatcg ctaattcgtc gtgaatcact
    10681 gaacgatgtt ccatgcgaac agatattcga tcatatcgaa atattaaaca ttacccctcc
    10741 ccacattggg caaaaaaaaa caaaaaacga tcaattaacg atcaattaac gatcaattag
    10801 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatataga
    10861 actgttactt ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata
    10921 ctttgttcat tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata
    10981 tagtgctgcc actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa
    11041 aacaaaccga aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc
    11101 agcaaatatt gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa
    11161 aggtattttc cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt
    11221 ctctttccat aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac
    11281 cgcaatagga ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc
    11341 agaaactcag gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga
    11401 ttttgagatt cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggaaga
    11461 gaccaaccct acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg
    11521 ttcaaaaaaa atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga
    11581 atccaaaatc tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga
    11641 ggtcacccaa tgggactcat acggcatgat gatttataaa aatgataaaa catttcgtaa
    11701 tcttgaaata ttcggagaca gcggctctgg agcatactta tatgataaca aactagaaaa
    11761 atgggtatta gtcggaacaa cccatggtat tgccagcgtt aatggtgacc aactgacatg
    11821 gataacaaaa tacaatgata aactggttag tgagttaaaa gatacctata gtcataaaat
    11881 aaatctgaat ggcaataatg taaccattaa aaacacagat ataacattac accaaaacaa
    11941 tgcagatacc actggtactc aagaaaaaat aactaaagac aaagatattg tgttcacaaa
    12001 tgggggaaat gttctgttta aggataattt ggattttggt agcggtggta ttatctttga
    12061 cgaaggccat gaatataaca taaacggtca gggatttaca tttaaaggag caggaattga
    12121 tatcggaaaa gaaagcattg taaactggaa tgcattgtat tccagtgatg atgttttaca
    12181 caaaataggc cccggtactc tgaatgttca aaaaaaacag ggggcaaata taaagatagg
    12241 tgaaggaaat gttattctta atgaagaagg aacatttaac aatatatacc ttgcaagcgg
    12301 aaatggtaag gtaatactaa ataaagataa ttcccttggc aatgatcaat atgcggggat
    12361 attttttact aaacgtggtg gcacgctaga tttaaatgga cacaatcaga cttttactag
    12421 aattgccgcc actgacgatg gaacaacaat aactaactca gatacaacga aagaagccgt
    12481 tctggcaatc aataacgaag actcctacat atatcatggg aacataaatg gcaatataaa
    12541 actaacgcac aatattaatt ctcaggataa gaaaactaat gcaaaattaa ttctggatgg
    12601 tagtgtcaac acaaaaaatg atgttgaagt cagtaatgcc agtcttacca tgcaaggcca
    12661 tgcaacagag catgcaatat tcagaagcag aacgagtcat tgctccctgg tatttctttg
    12721 tggaacggac tgggtcaccg ttttgaaaga aacagagagt tcatataata aaaagttcaa
    12781 ttctgattac aaaagtaata atcagcagac ctcatttgat cagcctgact ggaaaaccgg
    12841 ggtgtttaaa tttgatacat tacacctgaa caatgctgac ttttcaatat cacgcaatgc
    12901 caatgttgaa ggaaatatat cagcaaataa atcagctatc acaatcggcg ataaaaatgc
    12961 ttacattgat aatcttgcag ggaaaaatat tactaataat ggttttgact tcaaacaaac
    13021 tatcagtact aatctatcca taggagaaac taaatttaca ggtggcatca ctgcacataa
    13081 cagccaaata gccataggtg atcaagctgt agttacactt aatggtgcaa cctttctgaa
    13141 taatactcct ataagtatag ataaaggagc aaaagttata gcacaaaatt ccatgttcac
    13201 aacaaaaggt attgatatct ccggtgaact gactatgatg ggaatccctg aacagaatag
    13261 taaaactgta acgccgggtc tccactacgc tgctgatgga ttcaggctga gtggtggaaa
    13321 tgcaaatttc attgccagaa atatggcatc tgtcaccgga aatatttatg ctgatgatgc
    13381 agcaaccatt actctgggac agcctgaaac tgaaacaccg actatatcgt ctgcttatca
    13441 ggcatgggca gagactcttt tgtatggctt tgataccgcc tatcgaggcg caataacagc
    13501 ccccaaagct acagttagca tgaataatgc gatctggcat ctaaatagcc agtcatcaat
    13561 taatcgtcta gaaacaaaag acagtatggt gcgttttact ggtgacaatg ggaagtttac
    13621 aacccttaca gtggacaacc ttactataga tgacagtgca tttgtgctgc gtgcaaatct
    13681 ggcccaagca gatcagcttg ttgtcaataa atcgttgtct ggtaaaaaca acctcctgtt
    13741 agtcgacttc attgagaaaa atggaaacag caacggactg aatatcgatc tggtcagcgc
    13801 accaaaagga actgcagtag atgtctttaa agctacgact cggagtattg gcttcagtga
    13861 tgtaacaccg gttatcgagc aaaagaacga tacagacaaa gcaacatgga ctctgatcgg
    13921 ctataaatct gtggccaacg ccgatgcggc taaaaaggca acattactga tgtcaggcgg
    13981 ctataaagcc ttccttgctg aggtcaacaa ccttaacaaa cgtatgggtg atctgcgtga
    14041 cattaacggt gagtccggtg catgggcccg aatcatgagc ggaaccgggt ctgccggcgg
    14101 tggattcagt gacaactaca cccacgttca ggtcggtgcg gataacaaac atgaactcga
    14161 tggccttgac ctcttcaccg gggtgaccat gacctatacc gacagccatg caggcagtga
    14221 tgccttcagt ggtgaaacga agtctgtggg tgccggtctc tatgcctctg ccatgtttga
    14281 gtccggagca tatatcgacc tcatcggtaa gtacgttcac catgacaacg agtataccgc
    14341 aactttcgcc ggccttggca ccagagacta cagctcccac tcctggtatg ccggtgcgga
    14401 agtcggttac cgttaccatg taactgactc tgcatggatt gagccgcagg cggaacttgt
    14461 ttacggtgct gtatccggga aacagttctc ctggaaggac cagggaatga acctcaccat
    14521 gaaggataag gactttaatc cgctgattgg gcgtaccggt gttgatgtgg gtaaatcctt
    14581 ctccggtaag gactggaaag tcacagcccg cgccggcctt ggctaccagt ttgacctgtt
    14641 tgccaacggt gaaaccgtac tgcgtgatgc gtccggtgag aaacgtatca aaggtgaaaa
    14701 agacggtcgt atgctcatga atgttggtct caatgccgaa attcgcgata atcttcgctt
    14761 cggtcttgag tttgagaaat cggcatttgg taaatacaac gtggataacg cgatcaacgc
    14821 caacttccgt tactctttct gataacagcc cgggccgcgt ttgcggccct tcttctaccg
    14881 gagagaatat gtattaccct gtgacagact atatcgctct tgctctcatt attagctttg
    14941 aggtgtactg gcaatagcgg acactaccat ttgttctttt tttaagcagc catctgatga
    15001 tatttttccc tgaaggctgc cggggagata ttccccagac gagagtgacg acgctgacga
    15061 ttgtagaaaa tctcaatgta ttcccgtatt actgagatgg cttcatcccg gttattaaaa
    15121 cgatagt
//
LOCUS       DAETPZ010000088        15082 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.088, whole genome shotgun sequence.
ACCESSION   DAETPZ010000088 DAETPZ010000000
VERSION     DAETPZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.088"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            29..292
                     /locus_tag="K0W59_003152"
     CDS             29..292
                     /locus_tag="K0W59_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HBI3024088.1"
                     /translation="MSKLESLPTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLV
                     FAGVRLYMPALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            302..940
                     /locus_tag="K0W59_003153"
     CDS             302..940
                     /locus_tag="K0W59_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HBI3024089.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            943..2106
                     /locus_tag="K0W59_003154"
     CDS             943..2106
                     /locus_tag="K0W59_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBI3024090.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2190..3814
                     /locus_tag="K0W59_003155"
                     /pseudo
     CDS             2190..3814
                     /locus_tag="K0W59_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
     gene            complement(3931..4206)
                     /gene="yjfN"
                     /locus_tag="K0W59_003156"
     CDS             complement(3931..4206)
                     /gene="yjfN"
                     /locus_tag="K0W59_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HBI3024091.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4355..4684)
                     /gene="bsmA"
                     /locus_tag="K0W59_003157"
     CDS             complement(4355..4684)
                     /gene="bsmA"
                     /locus_tag="K0W59_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HBI3024092.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4866..5615
                     /gene="yjfP"
                     /locus_tag="K0W59_003158"
     CDS             4866..5615
                     /gene="yjfP"
                     /locus_tag="K0W59_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBI3024093.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5612..6367)
                     /gene="ulaR"
                     /locus_tag="K0W59_003159"
     CDS             complement(5612..6367)
                     /gene="ulaR"
                     /locus_tag="K0W59_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HBI3024094.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6475..7539)
                     /gene="ulaG"
                     /locus_tag="K0W59_003160"
     CDS             complement(6475..7539)
                     /gene="ulaG"
                     /locus_tag="K0W59_003160"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBI3024095.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCANDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7894..9291
                     /gene="ulaA"
                     /locus_tag="K0W59_003161"
     CDS             7894..9291
                     /gene="ulaA"
                     /locus_tag="K0W59_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBI3024096.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9307..9612
                     /gene="ulaB"
                     /locus_tag="K0W59_003162"
     CDS             9307..9612
                     /gene="ulaB"
                     /locus_tag="K0W59_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HBI3024097.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9622..10086
                     /gene="ulaC"
                     /locus_tag="K0W59_003163"
     CDS             9622..10086
                     /gene="ulaC"
                     /locus_tag="K0W59_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HBI3024098.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAVVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10100..10750
                     /gene="ulaD"
                     /locus_tag="K0W59_003164"
     CDS             10100..10750
                     /gene="ulaD"
                     /locus_tag="K0W59_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBI3024099.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10760..11595
                     /gene="ulaE"
                     /locus_tag="K0W59_003165"
                     /pseudo
     CDS             10760..11595
                     /gene="ulaE"
                     /locus_tag="K0W59_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
     gene            11595..12281
                     /gene="ulaF"
                     /locus_tag="K0W59_003166"
     CDS             11595..12281
                     /gene="ulaF"
                     /locus_tag="K0W59_003166"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HBI3024100.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTL
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12410..12685)
                     /locus_tag="K0W59_003167"
     CDS             complement(12410..12685)
                     /locus_tag="K0W59_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBI3024101.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            13012..13407
                     /gene="rpsF"
                     /locus_tag="K0W59_003168"
     CDS             13012..13407
                     /gene="rpsF"
                     /locus_tag="K0W59_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBI3024102.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNIEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13414..13728
                     /gene="priB"
                     /locus_tag="K0W59_003169"
     CDS             13414..13728
                     /gene="priB"
                     /locus_tag="K0W59_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HBI3024103.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13733..13960
                     /gene="rpsR"
                     /locus_tag="K0W59_003170"
     CDS             13733..13960
                     /gene="rpsR"
                     /locus_tag="K0W59_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBI3024104.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14002..14451
                     /gene="rplI"
                     /locus_tag="K0W59_003171"
     CDS             14002..14451
                     /gene="rplI"
                     /locus_tag="K0W59_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBI3024105.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14522..>15082)
                     /locus_tag="K0W59_003172"
     CDS             complement(14522..>15082)
                     /locus_tag="K0W59_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HBI3024106.1"
                     /translation="TMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDV
                     ESFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQL
                     RTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRS
                     DRMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3529 a         3671 c         4058 g         3824 t
ORIGIN      
        1 atcgggcatt atctgaacat aaaacactat cagtaagttg gagtcattac ctacattgtt
       61 aggttacgtt atcccattat ccagtgcggc aatcaatgcg gtgagtattc cagactattt
      121 cgcctggggc gggattgcac tggtgattca gttactcgtt tttgctggtg tcagacttta
      181 tatgcctgca ttaagcgaaa aaattattaa tcacaatacc gcagcaggaa tgtttatggg
      241 aaccgccgcg ctggctggcg gtatttttaa cgcagcttgt atgacatggt aatggacgat
      301 catggccaga aaacgcaaat caagaaataa cagtaaaatt ggtcacggag cgatcagtcg
      361 cattggtaga ccgaataatc cttttgaacc gcgtcgcaat cgctacgcac aaaaatactt
      421 aacgttggcg ctaatgggcg gtgccgcttt tttcgtattg aaaggttgta gcgatagtag
      481 cgatgtcgat aacgacggcg acggaacgtt ttacgcgaca gtgcaggatt gtattgatga
      541 cggtaataat gccgacattt gcgcgcgtgg ctggaacaac gccaaagcgg cattttatgc
      601 cgatgttcca aagaatatga ctcagcagaa ctgtcagtct aagtacgaaa attgctacta
      661 cgacaatgtt gaacagagtt ggatcccggt cgtttctgga tttttgttaa gccgggttat
      721 tcgtaaagat cgcgatgagc cgttcgtcta taacagcggc ggatcctcat ttgcttcccg
      781 ccccgtctgg cgcaacactt ccggtgatta ctcctggcgc ttcggttctg gcaaaaaaga
      841 gtcttactct tcaggcggct ttaccaccag aaaagcgtcg accgtttcgc gcggcggcta
      901 tggtcgttct tccagcgccc gtgggcattg gggaggctaa tcatgctgag acacaacgtt
      961 cctgtgcggc gggatctgga ccagatcgcc gctaacaacg gtttcgactt tcatatcatc
     1021 gacaatgaaa tctattggga cgagagtcgg gcttaccgtt ttactctgcg ccagattgaa
     1081 gagcagatcg aaaaaccgac tgcggaactg catcagatgt gccttgaggt ggtggatcgc
     1141 gcggttaaag acgaagagat cctgacgcaa ctggcgatcc cgccgttgta ctgggatgtg
     1201 atcgccgaaa gctggcgcgc acgcgatcct tcgctgtatg gccggatgga ttttgcctgg
     1261 tgtggcaatg cgccggtgaa gctgctggag tacaacgccg atacgccgac ctcgttgtat
     1321 gagtcggctt atttccagtg gttgtggctg gaggatgccc ggcgcagcgg cattattccg
     1381 cgtgatgccg atcagtacaa cgccattcag gaacgcctga tttcgcgctt tagtgagctt
     1441 tacagtcggg aaccgtttta tttttgctgc tgtcaggaca ccgatgaaga caggagtacc
     1501 gtgctgtact tgcaggactg cgcccagcag gcagggcagg agtcgcggtt tatctacatt
     1561 gaagatctcg gtttgggcgt cggcggcgta ctgaccgatc ttgatgataa tgtcatccag
     1621 cgtgcattta agctgtatcc gctggagtgg atgatgcgtg acgataacgg tccgctgctg
     1681 cgcaagcgtc gcgagcaatg ggtggagccg ttatggaaaa gtatcttgag taataaaggg
     1741 ctaatgccgc tgctttggcg cttcttccct ggtcatccta accttcttgc atcctggttt
     1801 gagggtgaaa aatcgcagat tgccgctggc gaaagctatg tgcgtaaacc gatctactcg
     1861 cgcgaaggcg gaaacgtcac cattttcgac ggtcagaata acgtcgttga ccacgctgat
     1921 ggtgattacg ccgatgaacc gatgatctac caggcgtttc aacctctgcc gcgatttggc
     1981 gatagctaca cactcatcgg tagctggatt gtcgatgatg aagcgtgcgg aatggggatc
     2041 cgtgaagata acacactgat caccaaagac acctcacgtt tcgttccgca ttacattgct
     2101 ggataagaat gttttagcaa tctctttctg tcatgaatcc atggcagtga ccatactaat
     2161 ggtgactgcc attgatggag ggagacacag tgcactggca aactcacacc atttttaatc
     2221 aacctatacc attaaataac agcaacttat acctgtctga tggcgcgctt tgcgaagcgg
     2281 taacgcgtga aggtgctggc tgggatagcg attttctagc cagtattggt cagcagttag
     2341 gaacggctga atcccttgaa ctggggcggc tggcgaatgt aaacccgcct gaactgttgc
     2401 gctacgatgc ccaggggcgg cgtctggacg atgttcgttt ccatcccgca tggcacctgc
     2461 tgatgcaggc gttatgtacc aatcgggtgc acaatcttgc ctgggaagaa gacgctcgct
     2521 ccggcgcatt tgtggcgcgc gcggcgcgtt ttatgttaca tggcaggttg aggctgggtc
     2581 gttatgtcca ataacgatga cctttgccgc cacgccattg ttgttacaga tgttacccac
     2641 gccgtttcag gactggacca cgccgctgct gagcgatcgc tacgattctc acttattgcc
     2701 aggtgggcaa aaacgcggtt tgttgattgg catgggaatg acggaaaagc agggcggttc
     2761 cgatgtcatg agcaacacca tccgtgcaga gcgtttggaa gatggctctt atcggctggt
     2821 ggggcataaa tggtttttct cggtgccgca aagcgatgcg catctggtgc tggcgcagac
     2881 cgcgggcggt ctgtcctgct tttttgtgcc gcgctttttg cctgacgggc aacgcaacgc
     2941 gattcgcctc gagcggctga aagataagct gggtaatcgc tctaacgcca gttgcgaagt
     3001 ggagtttcag gatgccattg gctggttatt ggggcaggaa ggggaaggaa ttcgtctgat
     3061 cctgaaaatg ggggggatga cgcgttttga ttgcgccctg ggtagccatg ccatgatgcg
     3121 ccgtgcattt tcgctggcga tttatcatgc acatcaacgc catgtttttg gtaatccatt
     3181 gatccaacag ccccttatgc gtcatgtctt aagtcgcatg gcacttcagc ttgaagggca
     3241 aacggcgttg ctgtttcgtc ttgcgcgagc gtgggaccgg cgtgccgatg ccaaagaagc
     3301 cctgtgggcg cgtttattta cgcctgcggc aaaatttgtg atctgcaaac gaggtatgcc
     3361 gtttgtggcc gaagcgatgg aggtgctggg cggcattggt tattgcgagg agagcgagct
     3421 gccgcggctt taccgggaga tgccggtaaa cagtatttgg gaaggttccg gcaatattat
     3481 gtgcctggat gtgttgcgcg ttctcaataa gcaagcgggc gtatacgact tattgtcgga
     3541 agcatttgtg gaagtgaaag ggcaggatcg ctattttgat cgcgcggttc gtcgtttaca
     3601 gcagcagctg cgtaagccag ctgaagaact ggggcgagag attactcatc agctattcct
     3661 gctgggctgc ggtgcgcaaa tgttgaaata tgcttctccg ccaatggcgc aggcgtggtg
     3721 tcaggtgatg ttagatacgc gcggcggcgt acggttgtca gagcagatcc agaatgattt
     3781 attgctgcgg gcgacggggg gagtgtgtgt gtaagcgtat acgactgatg cgacgctggt
     3841 ttcgattaac taaatgaaat atgtgaaaat tgtaggccgg acaaggcgct cgcgccgcat
     3901 ccggcattgt tcatttgctg tagatgacac tcatgcatac agtatcgcct gtacgcgcca
     3961 gttatcaggc tgctggtctt cgtacatggt aataatgcgg taccatgacg caccttgttc
     4021 atcggccttt aacgccacga tacgttccac gtcctgcgga tcccctgaaa tattgctaac
     4081 tgagatctga cctatttcat tcaggcccgt cacgcagtca gcactggcga attctgcaga
     4141 ctgtgccgtg gcgctcgcag gataagtcac taattgcagc gagggtgagg caagtaattg
     4201 tttcatcgtc agctccattt ttcgtttccc cacatgttgc agggcattcc atcgcggtaa
     4261 taaacgccca agcttccagc cggtaattta ttgtgcacag gtaaacgcat agggacgcaa
     4321 agcaatgtaa agctgactat gtaagttgtc tgatttattt acggtacaaa atggcttgtg
     4381 aataccactg tcctgtcacg atggtttcgt ccaccatgac gacaacgtaa taatcagctt
     4441 ttgcagcgac agcttttgct ctgatttctg ctaatgcgtc atccggagaa ccccgaacca
     4501 tcgtgcttac gctacctatt cgctgtaacc cttgcgtctg gtcgcggcga atctcttgcg
     4561 gatggtccgt tactggcggt gctggctgtg gcgtaccttg cagtgcgcta caggcactta
     4621 acatcaacac caataataaa ctggcaaacc ggtaaataac gctattacgt ttcctgctaa
     4681 ccatagtgta gtgccttatt aatttaactt tgggggaatg ttcccgaatt gttacctggt
     4741 acgcgatttt cgaatgaaaa cgtgacgaaa gcgtaaccca catctcaaca ttatgaacta
     4801 ataccgccag cagaaacttg tgctaaggat ttcttcatgt cataccagac aaaaaggaga
     4861 gacagatgat tgaaatagaa tcacgcgagc tggcagatat tcccgttctt catgcttatc
     4921 ctgtcgggca aaaagatacc ccgttaccgt gcgtaatttt ttatcacggc tttacttcat
     4981 ccagcctggt gtatagctat tttgccgttg cgctggcgca ggctggtttg cgggtgatca
     5041 tgccggatgc gcccgatcac ggtagccgtt ttagtggcga cgaagcgcgg cggttaaatc
     5101 aattctggca aatcttgcta caaagtatgc aggaattcac tactttacgt gcggcaatag
     5161 ccgaagaaaa ctggctgctt gatgaccgtc tggcagtcgg tggcgcgtcg atgggcgcga
     5221 tgacggcact gggaattacc gctcgccacc ccacggtgaa atgtaccgcc agcatgatgg
     5281 gatcgggcta ttttacatcg ctcgcccgtt cactgtttcc accgctgata cctgaaacgg
     5341 cagcacagca gaatgaattc aataaaattg tcgcgccgct ggcagagtgg gaagcgacaa
     5401 accacctgga acaacttggg gacagacctc tactgctgtg gcatggcctc gacgatgacg
     5461 ttgtgcctgc cgacgaatca ctacgtttgc agcaggcctt aagcgagacg ggacgggata
     5521 aactgctaac ctgttcatgg cagccaggcg tgcgtcaccg cattacgcct gaggcgttag
     5581 atgctgccgt gacatttttc cgccagcatc tttaaacacg cagaatgctg accccttgcg
     5641 cttccagttg ttgcaggatt tccgggttag catttttgcc ggtgatcagc atatcgattt
     5701 gatcggcacg gctaaaaagc attcccgcgc gttcgccaat cttactgcta tcaaccagta
     5761 ccaccagttt ccctaccacg ctcagcatct tctgctctgc cattgctgtc agcatatcgg
     5821 ttttatacaa cccttctgcg gtcagccctt ttccgctggt aaacatccag tgcccggcat
     5881 agagactgtt ttcgctgccc tgcgggctta aagtgatgga ctgacttttg ttgtactgtc
     5941 cacccataat gatcacgctg tcatgttctt gatcgatcag gtaatttgcc agcggtagat
     6001 aattagtgat gatttgcact ggcttgccac acatttcccg cccaagcaga aacgcggtgg
     6061 agccgcagtt gatgactacg ctttcgcccg gattaaccag ctgcgaggcc gctttagcaa
     6121 tacgtacttt ttcatcgtga ttctgcgcct gatgcagatt catcggcgtc cagcgcgggc
     6181 gctgttgggt aatagcttct gcgccattgc gcaccttttt cagtttgccg ctttcgccaa
     6241 gtttattgat atcgcgtcgc gcagtggcag gcgaaattcc cagacgctca acgactttct
     6301 caacggtcac aaagcccaat tgtgcgagca tttccaggag gatttgatgt ctttgtgctt
     6361 cagtcatgag ctattccgat aaaatttgat ttttttagat gatatttgaa atagccagga
     6421 aatactacgc cggatagcgc gaacttctcc acgctatccg gtataaacga cagattacag
     6481 gaatgacttg aacggcagat ccggttcaat agtgaagcaa tcatcgaaac cgcgcggata
     6541 gtggtactcg aagttgtctt tatccagcgg ccaggtaaat ttgccgccaa cctgccagat
     6601 aaacggcttg aagccatact tcagacgatc ttttttcatc tcccacagca cgcggatctc
     6661 ttgcggatcg gcctggaagt ttgaccagat gtcgtggtgg aacgggataa ccactttcgc
     6721 attcagggct tcacccatac gcagcatatc ggcgctggtc attttgtcgg taataccgcg
     6781 cgggttctca ccatacgatc ctaacgccac atcgatctga tgttcgttac cgtgcttcgc
     6841 ataatagtta gagtagtggg aatcgccact gtgatatagg gaaccgccag gggttttgaa
     6901 caggtagttt actgcgcgat cgtccatgcc atctggcagt acgccagccg ctttttgatc
     6961 ggcaggcagg gtgatcagtg cagtacggtc gaaagcgtca agcgcatgaa tttcaatgtc
     7021 tttcactttt acaacatcgc ccggtttgac cacgatgcaa cgctctttcg gtacgcccca
     7081 gccaatccac aaatcaacgc aggttttcgg tccaatgaat ggtacgtcat tagcacagtt
     7141 ctgcatcacg gcagcagcga cgttaacgtc gatatgatcg ttgtgatcgt gagtcgccag
     7201 taccgcgtcg atctggcgaa tcgcaaacgg atcaagaaca aacggggtgg tgcgtaggtt
     7261 tggctgcagt tttttcacgc cagccatgcg ctgcatctgg tgaccctgtt tcattaacgg
     7321 gttaccgtga ctttgtttgc cagtgccgca ccagaaatca acgcaaacgt tggtgccacc
     7381 ttctgatttc aaccagatcc cggtgcagcc tagccaccac atcgcaaatg tgccaggggc
     7441 gacctgctct tgttcaattt cttcattcag ccagctaccc cactccggga aagtgctcag
     7501 gatccaggat tctcgggtga tacttttcac tttactcatc gccatttacc ttcatgatag
     7561 ttcaattcga atcaatatgt gattggtttt gattaatcct gacactattt tttcaggaag
     7621 gcaatgacca ttttttgact ttttgccagg gaagttgttg ttgattttga gtgtagaaag
     7681 atttaatgga atgtatagat aaattaacta aatgaattta aataaataat tttttcgttg
     7741 tgtgaatccc actctatccg tgtggaatta tttgcgggtc gcgccacatt taatcataaa
     7801 taatcttgtt gtgattactt ttgaaaatta gagtgagtgc acaacattcc gggtgtgcgg
     7861 aatacccggt tacctcttct tcaggagatc gttatggaga tcctctacaa catctttacc
     7921 gtgtttttta accaggtcat gaccaatgcc ccgttgttgc tgggtattgt gacctgtctg
     7981 ggctacatcc tactgcgcaa aagtgtcagc gttattatta aaggcacgat taaaaccata
     8041 attggtttca tgttgttgca ggcagggtcc ggcatcctca ccagcacctt caaaccggtg
     8101 gtggcgaaaa tgtccgaagt ctacggcatt aacggcgcaa tttccgatac ttacgcgtcg
     8161 atgatggcaa ccatcgaccg catgggcgat gcctatagct gggtaggtta cgcggtgttg
     8221 ttagcgctgg cgctgaacat ctgttacgtg ctgctgcgtc gcattaccgg tattcgtacc
     8281 atcatgctga ctggtcatat catgttccag caggcggggc tgattgccgt tacgttgttt
     8341 attttcggct actccatgtg gacaaccatt atctgcacgg cgattctggt ttcgctctac
     8401 tggggcatta cctccaacat gatgtacaag ccgactcagg aagtgacgga cggctgcggt
     8461 ttctccatcg gtcaccagca acagtttgca tcattaattg cctataaagt tgcgccgttc
     8521 ctcggcaaaa aagaggaaag cgttgaagac ctcaaactgc caggctggct gaatattttc
     8581 cacgacaaca tcgtctccac ggcgattgtc atgaccatct tctttggtgc cattctgctc
     8641 tccttcggta tcgacaccgt gcaggcgatg gcaggcaaag tgcactggac ggtctatatc
     8701 ctgcaaaccg gtttttcctt cgcggtggcg atcttcatca tcactcaggg tgtgcgcatg
     8761 tttgtggcgg aactctctga agcgtttaac ggtatctccc agcgcctgat ccctggtgcg
     8821 gttctggcga ttgactgtgc ggctatttat agcttcgctc cgaacgccgt ggtctggggc
     8881 tttatgtggg gcaccatcgg tcagctgatt gcggttggca tcctggtcgc ctgcggttcc
     8941 tcgatcctga ttatcccagg ctttatcccg atgttcttct ccaacgccac catcggcgtg
     9001 ttcgctaacc acttcggcgg ctggcgtgcg gcgctgaaga tttgtctggt gatggggatg
     9061 atcgaaatct ttggttgcgt ctgggtggtg aaacttaccg gtatgagtgc ctggatgggg
     9121 atggcggact ggtcgattct ggcaccgccg atgatgcagg gcttcttctc catcggtatc
     9181 gcctttatgg ccgtcatcat tgtaattgca ctggcttata tgttcttcgc tggccgcgca
     9241 ctgcgcgcag aagaagatgc agaaaaacaa ttggcagaac agtctgctta ataaggagtt
     9301 ttgattatga ccgtacgtat tctggctgtg tgtggcaacg gacaaggcag ttccatgatc
     9361 atgaagatga aagtggacca gtttttaacc caatcaaaca ttgaccacac ggtaaatagc
     9421 tgcgcggttg gcgaatacaa aagcgagttg agcggcgcgg atatcatcat cgcttctacg
     9481 cacattgcgg gcgaaatcac cgtgaccggc aacaaatacg tggttggcgt gcgcaacatg
     9541 ctctctcctg ccgactttgg cccgaaactg ctggaagtga tcaaagcgca tttcccgcag
     9601 gatgtgaagt aaggacgcgc catgaaatta catgattcgc tggcggagaa taaatccatc
     9661 cgcctgcagg ctgaagctga gacatggcag gacgcagtaa aaatcggcgt tgacctgctg
     9721 gtggcggcgg atgttgtcga gccgcgttac taccaggcga ttctggatgc ggttgaacag
     9781 cacggtccgt actttgtgct cgcaccaggc ctggcaatgc cgcacggtcg cccggaagag
     9841 ggcgttaaga aaaccggttt tgcgctggtg acgctgaaaa agccgctgga gttcaaccac
     9901 gaagataacg acccggtgga tatcctcatc accatggcgg tggtcgatgc caacacgcac
     9961 caggaagtgg gcatcatgca gatcgtcaac ctgtttgaag atgaagaaaa tttcgaccgt
    10021 ttacgtgcct gccgtaccga gcaggaagta ctggatctca ttgaccgcac caacgcggca
    10081 gcttaagaag gaattgaaga tgtcattacc gatgttgcaa gtcgcgctgg acaaccagac
    10141 tatggatagc gcctacgaaa ccactcgcct gattgccgaa gaagtcgaca ttatcgaagt
    10201 gggtaccatt ctgtgcgtgg gcgaaggcgt gcgtgcggtt cgcgatctga aagcgctcta
    10261 cccgcacaaa atcgtgctgg cagacgccaa aattgccgat gcgggcaaaa ttctttctcg
    10321 tatgtgcttc gaagccaacg ctgactgggt gaccgtgatt tgttgtgcgg atatcaacac
    10381 cgccaaaggt gcgctggacg tagcaaaaga gtttaacggc gacgtgcaga tcgaactgac
    10441 cggttactgg acctgggaac aggcgcaaca gtggcgcgac gcaggtattc agcaggtggt
    10501 ttatcaccgc agccgtgacg cgcaggccgc tggcgtggcg tggggtgaag ccgatatcac
    10561 tgcgatcaaa cgtctttccg atatgggctt caaagtcacc gtcaccggtg gcctggcgct
    10621 ggaagatctg ccgctgttca aaggcattcc aattcatgtg tttatcgccg gtcgcagcat
    10681 ccgtgatgcc gcttctccgg ttgaagccgc gcgtcagttc aaacgttcca tcgccgaact
    10741 gtggggctaa ggagcggata tgttgtccaa acaaatcccg cttggcatct atgaaaaagc
    10801 gctccccgcc ggggagtgct ggctggaacg cctgcgactg gcaaaaacgt tgggcttcga
    10861 ttttgtcgag atgtcggtag atgaaactga cgagcgcctg tcgcgcctcg actggagccg
    10921 cgagcagcgt ctggcgctgg tgaatgcgat tgttgaaacc ggcgtgcgtg tgccgtccat
    10981 gtgcctttct gcccatcgtc gtttcccgtt gggcagcgaa gatgacgcgg tgcgggcgca
    11041 ggggctggag attatgcgta aagccatcca gttcgcccag gacgttggca ttcgcgtgat
    11101 ccagctgcgg gctatgacgt ttactatcag gaagccaata acgaaacgcg tcgtcgtttc
    11161 cgtgacggcc tgaaagagag cgttgagatg gcgagccgcg cacaggtgac gctggcgatg
    11221 gagatcatgg attatccgtt gatgaactcc atcagcaagg cgctgggtta cgcgcactat
    11281 ctcaacaacc cgtggttcca gctctacccg gatatcggca acctgtcggc gtgggacaac
    11341 gatgtgcaga tggagttgca ggccggaatc gggcatatcg tcgcggtgca tgtgaaagac
    11401 accaaaccgg gcgtcttcaa aaacgtgcca tttggcgaag gtgtggtgga tttcgaacgt
    11461 tgcttcgaaa cgctcaaaca gagtggctat tgcgggccgt acctgattga gatgtggagc
    11521 gaaacggcgg aagacccggc ggcagaagtg gtgaaagcgc gcatggccaa agcaggcatg
    11581 gtggaggcgg cataatgcaa aagctaaaac agcaggtgtt tgaagccaac atggatctgc
    11641 cgcgctatgg gctggtgacc tttacttggg gcaacgtcag cgctatcgac cgcgaacgcg
    11701 ggctggtggt gatcaagccc agcggcgttg cctacgaaac catgaaagca gacgatatgg
    11761 tggtggtcga tatgagcggc aaggtggtgg aaggggagta tcgcccatct tccgacactg
    11821 cgacgcatct cgaactctac cgtcgttacc cgtcgcttgg cggcattgtc catacccact
    11881 ccactcatgc caccgcatgg gcgcaggcgg ggctggcgat cccggcatta ggcaccacgc
    11941 acgctgacta cttctttggt gatatcccgt gtacgcgtgg attaagcgaa gaagaggtgc
    12001 agggtgagta tgaactgaac actggcaaag tgattatcga aacgctgggc aacgccgagc
    12061 cgctgcatac gctgggaatt gtggtgtatc agcacggacc tttcgcctgg gggaaagatg
    12121 cccacgacgc ggtgcataac gcggtagtga tggaagaagt ggcgaaaatg gcgtggattg
    12181 cgcgcagtat taatccacaa ctcaatcata tcgacagcta cctgatgaat aaacacttca
    12241 tgcgtaagca cggtcctaac gcttattacg ggcagaagta aaacacgcgc tgcggaaatt
    12301 tccttcctcg ggagataact ggtctaattc cgcagccgtt tttcaaaaaa agccccctgc
    12361 gaagggggca aagcaaacta tggcaatgtt tcgttggtta tacctggtgc tagcgataaa
    12421 tatccgcgct ggcgtgcata ttgccgttac tccccggttc ccgcatcaga attacgtggt
    12481 agtacgttgc gccttgcgca tctgtttctt catttaatgc ctgacgtgct tcgctttcag
    12541 tggcgaaatt atgattgata taaatcacgc ctaagctttg cacatcgtcc atatttctgg
    12601 cctggtggtt attaatttca atggctgccc atgtatttgc acttagcaaa agcacagcca
    12661 gaagggctaa aactcgactg aacatagata cctcctcgac ggctgacttt gtgtgctctc
    12721 ctctgtgatg atcttctgat ttaattttaa tcaatgataa agaagttgat ggtgaccatt
    12781 tctgatgcag ttgttcaaaa aaacactgtg acggaatgtg atgaacttca aatcagcggt
    12841 ttagaggtta tttgcgaaag gggagattta tttctgctcc gcccttgagt ttagcggggt
    12901 atgtcagtac tataacgcgt catttttcag ccgaccttta acacgttcct tgcctccccg
    12961 ggattcggct gacccagaca ggaggctgaa taatccgtaa ggagcaattc gatgcgtcat
    13021 tacgaaatcg tttttatggt ccatcctgac cagagcgaac aggttccggg catgatcgag
    13081 cgctacactg ctgccatcac tggtgcagaa ggcaagatcc accgtctgga agactggggc
    13141 cgccgtcagc tggcttaccc gatcaacaaa ctgcacaaag cacactacgt tctgatgaac
    13201 attgaagctc cgcaggaagt gatcgatgag ctggaaacta ccttccgctt caacgatgcc
    13261 gttatccgca gcatggttat gcgtaccaag cacgctgtta ccgaagcatc tccgatggtt
    13321 aaagcgaaag acgagcgccg tgagcgtcgc gatgatttcg caaacgaaac cgctgatgat
    13381 gctgaagctg gggattctga agagtaattt ctgatgacca accgtctggt gttgtccggc
    13441 accgtgtgca ggactcccct tcgaaaggtc agtccatcag gaattcctca ctgccagttc
    13501 gtgcttgagc atcgttctgt gcaggaggaa gccggctttc accggcaggc atggtgtcaa
    13561 atgcccgtta ttgttagcgg acacgaaaac caggccatta ctcacagtat aacggtcggc
    13621 agtcgcataa ccgttcaggg gttcatttca tgccacaagg caaagaacgg actgagcaaa
    13681 atggttttgc acgccgagca gattgaattg atagattctg gagactagcc atatggcacg
    13741 ttatttccgt cgtcgcaagt tctgccgttt caccgcggaa ggcgttcaag agatcgacta
    13801 taaagatatc gctacgctga aaaactacat caccgaaagc ggtaagattg tcccaagccg
    13861 tatcaccggt acccgtgcaa aataccagcg tcagctggct cgcgctatca aacgcgctcg
    13921 ctacctgtcc ctgctgccgt acactgatcg ccatcagtaa tcggtcacgg tccattaata
    13981 cgactttgag aggataaggt aatgcaagtt attctgcttg ataaagtagc aaacctgggt
    14041 agcctgggtg atcaggtaaa cgttaaagcg ggctatgctc gtaacttcct ggtaccgcag
    14101 ggtaaagctg ttccagctac caagaaaaac attgaattct tcgaagcacg tcgcgctgaa
    14161 ctggaagcta aactggctga agttctggca gctgctaatg ctcgcgctga gaaaatcaat
    14221 gcactggaaa ctgttaccat cgcgtctaaa gctggcgacg aaggtaaact gttcggttcc
    14281 atcggtactc gcgacatcgc tgacgctgta actgcagctg gcgttgaagt ggctaagagc
    14341 gaagttcgtc tgccgaacgg cgttctgcgt accactggtg aacacgaagt gagcttccag
    14401 gttcacagcg aagtattcgc gaaagtgatc gtaaacgtag tagctgaata attcgttatt
    14461 caacgagacg taaaaacgcc gaccattggt cggcgttttg ctttctattt ttcgtcaggc
    14521 attaatttcg caagtagatc cattcaccat tctcttgtgt ccataagtca ggatggtttg
    14581 ctggcgttag atgcatacga tcactcctta tgtctcggtc ctgcggcact cgctcgaaac
    14641 ctaaacccga gtagattgcg gctgaatttg ataaggaacg tagaccaatt ttcaaatcat
    14701 ggggtaaatc agaatacgcg tttagcaatg cagcaatgat gaatccgagg gcattagaaa
    14761 cgcctgtaac agtgcgaagt tggtcgatat aaagatcgat tgttggtagt tcagatgacc
    14821 agcaattagg catttgggca ggaagggtta caacgtatcc tatcgtttgg ccggtatagt
    14881 ctggagtact ataaccaggt cccaaaacaa cctcgtgtat tgtgtattta ctgttgggtt
    14941 cgaaggattc gacatcttgc aataaatatt tcagttctgc ggttaacctc gcacccgcgt
    15001 aagctttatt gtatttctgg gcgtgaatat tatctaatgt agcttcatgg ttccatccaa
    15061 aagaattatc accattcatt gt
//
LOCUS       DAETPZ010000089        14927 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.089, whole genome shotgun sequence.
ACCESSION   DAETPZ010000089 DAETPZ010000000
VERSION     DAETPZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14927
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.089"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            83..364
                     /locus_tag="K0W59_003173"
     CDS             83..364
                     /locus_tag="K0W59_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024107.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRVEMYREKEKITYHWKKPERLRR"
     gene            complement(1161..2621)
                     /gene="phoQ"
                     /locus_tag="K0W59_003174"
     CDS             complement(1161..2621)
                     /gene="phoQ"
                     /locus_tag="K0W59_003174"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HBI3024108.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(2621..3292)
                     /gene="phoP"
                     /locus_tag="K0W59_003175"
     CDS             complement(2621..3292)
                     /gene="phoP"
                     /locus_tag="K0W59_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HBI3024109.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(3461..4831)
                     /gene="purB"
                     /locus_tag="K0W59_003176"
     CDS             complement(3461..4831)
                     /gene="purB"
                     /locus_tag="K0W59_003176"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBI3024110.1"
                     /translation="MELSSLTAVSPVDGRYGDKLSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(4835..5476)
                     /gene="hflD"
                     /locus_tag="K0W59_003177"
     CDS             complement(4835..5476)
                     /gene="hflD"
                     /locus_tag="K0W59_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HBI3024111.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(5512..6618)
                     /gene="mnmA"
                     /locus_tag="K0W59_003178"
     CDS             complement(5512..6618)
                     /gene="mnmA"
                     /locus_tag="K0W59_003178"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBI3024112.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(6672..7133)
                     /gene="nudJ"
                     /locus_tag="K0W59_003179"
     CDS             complement(6672..7133)
                     /gene="nudJ"
                     /locus_tag="K0W59_003179"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HBI3024113.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(7143..7796)
                     /gene="rluE"
                     /locus_tag="K0W59_003180"
     CDS             complement(7143..7796)
                     /gene="rluE"
                     /locus_tag="K0W59_003180"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HBI3024114.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            7968..9218
                     /gene="icd"
                     /locus_tag="K0W59_003181"
     CDS             7968..9218
                     /gene="icd"
                     /locus_tag="K0W59_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HBI3024115.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(9321..9644)
                     /gene="iraM"
                     /locus_tag="K0W59_003182"
     CDS             complement(9321..9644)
                     /gene="iraM"
                     /locus_tag="K0W59_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HBI3024116.1"
                     /translation="MKWAVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGRIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESEITNKCLHKSCIIKIC
                     PYGLK"
     gene            complement(9897..9941)
                     /locus_tag="K0W59_003183"
     CDS             complement(9897..9941)
                     /locus_tag="K0W59_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024117.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            complement(10177..10287)
                     /locus_tag="K0W59_003184"
     CDS             complement(10177..10287)
                     /locus_tag="K0W59_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024118.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQAHTIKLIFEM"
     gene            complement(10340..10744)
                     /locus_tag="K0W59_003185"
     CDS             complement(10340..10744)
                     /locus_tag="K0W59_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HBI3024119.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNQLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(10965..11696)
                     /gene="bluR"
                     /locus_tag="K0W59_003186"
     CDS             complement(10965..11696)
                     /gene="bluR"
                     /locus_tag="K0W59_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HBI3024120.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRHYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFETRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            complement(11901..13112)
                     /gene="bluF"
                     /locus_tag="K0W59_003187"
     CDS             complement(11901..13112)
                     /gene="bluF"
                     /locus_tag="K0W59_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HBI3024121.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMTHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            13426..13662
                     /locus_tag="K0W59_003188"
     CDS             13426..13662
                     /locus_tag="K0W59_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HBI3024122.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSHCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            13705..13977
                     /locus_tag="K0W59_003189"
     CDS             13705..13977
                     /locus_tag="K0W59_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HBI3024123.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKLAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            14006..14272
                     /gene="ariR"
                     /locus_tag="K0W59_003190"
     CDS             14006..14272
                     /gene="ariR"
                     /locus_tag="K0W59_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HBI3024124.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            14385..14633
                     /locus_tag="K0W59_003191"
     CDS             14385..14633
                     /locus_tag="K0W59_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024125.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT         4004 a         3402 c         3476 g         4045 t
ORIGIN      
        1 catgaggatt ttgcttttga tataagcgac agccaccgcc agaaactgga agaatttagc
       61 ggggtgtaat gaggtcagca gcatgacaga agccgaaata acgggattaa tccgccgcgt
      121 tgccggaatc agccagcagg ctgacgaaca ggccacgcag ccggacagcg tgacagccga
      181 aaactatgcg cgtgttgttg ctgaggtgat gcgccgtgat ggtatagaac ttaatggcgt
      241 ggatatgcgc aacatacgaa ccagagttct tgagttgctg gcctaccgtc gccgcgtgga
      301 gatgtatcgg gagaaggaga aaatcacgta ccactggaag aagccggagc ggttacggcg
      361 gtaactcact gagattaaag caaagcgcaa aattgcgctg gctacatccc tcacatctga
      421 ggactgttaa ccagcaggcc ggaatatatt cagaaactac gaaattttcg cagtttaatt
      481 actcgtcagc ttaatcgcta acccgctctg atattttcgg aaacctcaat ttgaggaagt
      541 cggcgaggta actgtttgat attcccgata gcgcaaaaat gcgcggtcgg gcagaatgac
      601 caccacctca atcgtgacgg agagtgacaa ccagtgacac accgtgacaa aaagacggtt
      661 taaccgttca ttaaaaatcg acgtgatttt gtgctttccg gatgacgtgg ccaacgtcat
      721 tttttagcga aaaattctga ttaaggtcac tggctgaacg gtcataatga ccacgcagac
      781 catcgaggca accatagcga tcatcgttac ggttggagct tttccgccat tggtggaaaa
      841 ggtgcacatc tggatcatca ttacggtgat caagggtatg taaaacccac cagcctgatt
      901 aaaggttaac cggaaaaaaa atccggtagc cccgttatgg ggatccccat atcgacatta
      961 acgcccctca tgaattggtg cgcttccccc tggaaagatt acccgcctgt atatttcttg
     1021 tgtctatttg ttccacgttg tttcatacag tgcaccgaac ggtgtagtta atggtgtagt
     1081 caatttgcga tttttggcac tttttcaatg aaaattgttt aatcgaatca atgacttgat
     1141 gtagtggtaa aaggacatat ttattcatct ttcggcgcag aatgctggcg accaaaaatc
     1201 acctccatcc gcgcaccgcc cagcatgctc tctccggcga cgattttacc ctcatattgc
     1261 tcggtgattt cgcgggctac cgccagccct acaccttgcc cagggcgtaa agtatcaacc
     1321 cgttgaccac ggtcgaaaat gacctctcgc ttgcttaatg gaataccggg gccatcatcc
     1381 tcgaccacaa tatagagatg ctcgtcggtt tgccttgcag aaatttcgac aaactcgagg
     1441 caatatttac aggcattatc cagcacgttg cccatcacct cgacaaaatc gttctgctca
     1501 ccgacaaagc tgatctctgg cgaaatatcg agagagatat tgaccccttt gcgttgatac
     1561 actttgttca gcgctgaggt gagattgtcc agcagtgggg cgaccggatg cagctcgcgg
     1621 ctgagcaatg tcccgccgcg catactggca cgatgcaggt agtagccaat ttgctgtgaa
     1681 atgcggctga tttgctccag cattaccggc tcagcatcac tgacgctcat cttttcacta
     1741 cgcagagaac gcagcgtact ttgcagcacc gccagtggcg ttttcagact atgggtcagg
     1801 tcggtgagcg tcgtacggta tttgtcgtaa cgttcgcgtt cactttttaa caatcggttc
     1861 aggtttcgta ccagactggt cagttctcgc gttgtggctg gattgagcaa ttcgcggtta
     1921 tgttcttcca gttcgcggac ttcttttgcc agggcttcga tggggcgtaa actccaccag
     1981 gcggcgaccc acagcagcgg gatcactaac agcagattgg ctgagagcac atagataaac
     2041 cagctccaga ccatatagga actttttagc tccaccggaa tggtatccac caccacaatg
     2101 gttaattttg gcatccgcga tgttgccggg tagacgttta ccgccaccga gtgggtcatc
     2161 tctgcgtcgt catcatcttc ccgcacttcc tgcaactgtt gctgtatcga atgatctcca
     2221 ctcagcaaga ggctggtatc gttaacatcc gcttcaattt catgaaaacc attcgatttc
     2281 agccagtcag gctggatcat tttcatcagc cagggcacgt cacgttgcgc ccataaaagc
     2341 tgcccgttct catcataaat tagcgtcatg gtggggcttt gcttgtcgat attttcgggt
     2401 aactcgacat gcaatttatt gttttcccac tgcgcaaggg tatagaacag attgctctcg
     2461 ccacgtaaca gccgaaacgt agttttatcg aaactgacgc tataaccgat cagcgcgacc
     2521 attccgtagg caagcgaaag caccagtact accgctgccg ttgccaacag aaaacgtacc
     2581 cgcagcgaga gcgggaaaaa aagacgcagt aattttttca tcagcgcaat tcgaacaggt
     2641 agccctggcc gcgaacggtg gtaatgactt cttgcggata ttgtgcctga atttttttgc
     2701 gcagacgtcc catcagtaca tcaatggtat ggctttcccg cagctccgca tccggataga
     2761 gttgtagcat taacgaatct ttgctgacca ctttgccatt attgcgtatc aacgtttcca
     2821 taatggtgta ttcgaacgcg gtcagtttga tcacttcgtc attaatagat aattcacggc
     2881 gagagagatc aacctgaaac ggggggagcg aaatgacctg tgaagccaga ccgctattac
     2941 gccgcattaa tgcctgcatt cgcgccatca cctcttcaat atgaaacggt ttagtcacgt
     3001 aatcatcagc accggcactt aatacttcga ctttgtcctg ccagctttca cgggcggtta
     3061 ataccagaat cggcagtgaa acatcgttgc tacgccagcg gcgaatcagt gacagaccgt
     3121 cctcgtcagg caatccgaga tcgacaatcg caatatccgg taaatgttca ttgagataat
     3181 aatcggcttc tttggcatct tctgcgtcat cgacctgatg accagcatcc tgaatctgaa
     3241 ctttaaggtg gtgacgtaac aacgcattgt cttcaacaac cagtacgcgc atttttattt
     3301 ctccctgtct taattattaa aatagtgtaa cgcgattatg tgtttatggg ggtaaacatt
     3361 aaataaacca gcggggaggg gaggtaaagt gaaaaaagaa aaagtggata atcttaataa
     3421 gcaggccgga cagcatcgcc atccggcact gatttgaggc ttatttcagc tcatcaacca
     3481 tcgtgatagc tcgaccaata tagttagccg gcgtcatcgc tttcaggcgg gctttctctt
     3541 cttctggcaa cgccagacca tcgataaact gcttcatgcc ttcggcgtca acgcgcttac
     3601 cgcgagtaag ctctttcagc ttctcgtacg gtttttcgat gccatagcga cgcataactg
     3661 tctggattgg ttctgccagc acttcccagt tgtgatccag ctcatccagc agatggtcac
     3721 ggttcacttc cagtttgctc acgcctttca gggtggattg atacgcaatc agcgcataac
     3781 cgatacccac gccgaggtta cgcagcacgg tggagtcggt caggtcacgc tgccagcggg
     3841 aaaccggcag tttgcttgcc agatgctgca ataccgcgtt ggaaaggccc agattccctt
     3901 cggagttttc gaagtcgatc gggttaactt tatgcggcat ggtggaagaa ccaatctcac
     3961 cagcaatggt tttctgtttg aagtggttaa gggcgatata accccagacg tcacggtcaa
     4021 agtcgatcag aatggtgttg aagcgcgcaa cgcaatcaaa cagttcggca atgtagtcgt
     4081 gcggttcaat ctgggtagtg tacgggttcc attgaatacc cagcgaggtg acgaactctt
     4141 cgctgaactg atgccagtca acttccgggt aagcggcgat gtgggcgtta tagttaccga
     4201 ccgcgccgtt gattttgccg aggatctcca cctggttaag ctggcggtgc tggcgctcca
     4261 tacggtaggc gacgtttgcc atctctttac cgatggttga cggcgtggct ggctgaccgt
     4321 gggtacgaga cagcagcggg atatcgcggt actgaacggc gagatcttta atgccatcaa
     4381 tcagttgacg ccagtacggc aggatcactt catcacgcgc ggttttcagc attaatgcgt
     4441 gggagaggtt attgatatct tccgaagtac aggcaaagtg gatgaattca gaaaccgcgt
     4501 gcagttccgg gatctccgcc actttttctt tcaggaaata ctcaaccgct ttaacgtcgt
     4561 ggttagtggt acgctcgatg gttttgatgc gtgcggcatc ttcttcgctg aaattggcga
     4621 caattgcatc aaggtaaccg attgcgtcgg cagcaaaagc aggaacttcc ttgatcgctg
     4681 cgtgcgcggc cagtttttgc agccaacgta cttcaacttg tacacggaat ttcagcaaac
     4741 catattcgct gaaaatcccg cgcagcgcgc tgagtttatc gccgtagcgt ccatcgacag
     4801 gggaaacggc ggtcagtgag gataattcca tagatcacaa ctccggggtt aaatgagcaa
     4861 gaatttgttt tgcctgagtg gtcaggcgat tacgagaaaa catcagttgt agacgtccgc
     4921 cgccgacctg gtgccagagc acggcggcgc gaatgcctgc cagcagggtt gcgcgaactt
     4981 tcgcctgcac ttgtgggctt tgcagtacag caggggaacc ggtgacctga atgcgcgggc
     5041 caagcgggct gatcacatca acatagatag cagccatcgc gctcatcagc gtttcggact
     5101 gtaaatcgaa gtgttcgagc tggcgttgca ggccgttgat ccggttgccc agagtgtcga
     5161 gcgcgccttt cgctgaggag agtttgcgct caagcaccat caagctgagt gtgtagcggg
     5221 ttaattcggc gtttaagccc tggcaactgc tggcattgag cacgccgagc agggtttcca
     5281 gcccgacgcg caggttggct tcgctaccgc caaaaaccgc cagcgtcgag ctggggttca
     5341 tatcaataat actgttgagt gagacgtgta gcgcatcggc atcacaatgc ccctggtgag
     5401 cgagttgttg caccaggcgt gccgactgac aaataccggc cagggcgagg gtgatgtcat
     5461 agtaattctt tgccacgttc actgcttcct tgttttaagt aaagataata atcagaccgg
     5521 cagcggcaga cgctgctcaa taataccgcc accgaggcac acttcaccgt tatagaagac
     5581 ggcagactgg cccggcgtca cggcggcaac cggctcatcg aaaatcactt taatgcgatc
     5641 gtcgtccagc gccttgacgg tgcacggaat gtcggtctgg cgatagcggg tttttaccgt
     5701 gcaacgcata gtgccggtga atggttcgcg atcgacccag tgcagctgct gggcaatcaa
     5761 cccgacagac atcagccgcg ggtgttcatg gccctgagcg acaaccagaa tgttgttttc
     5821 gacgtctttg tccaccacat accacggttc ttcggtgcct tctttggtgc caccgatacc
     5881 cagaccttta cgctgaccga gagtgtgata catcagcccc tggtgctcac caatttcatc
     5941 gccatcgacg gtaatgattt tgcccggttg cgccgggaga taacggccca ggaactcgcg
     6001 gaatttacgt tcgccaatga agcaaatgcc ggtagagtct tttttcttcg cggtgaccag
     6061 accaagatct tcagcaatct tacgcacctg cggtttttcc agttcgccga ccgggaacag
     6121 gctttgcgca atctgctcat ggctgagcgt ataaaggaag tagctctggt ctttattgct
     6181 gtccagacca cgcagcaggc ggctcttgcc atcgacatcg gcccgacgta cgtaatgacc
     6241 ggtagcgata taatcggcac ctaaatcttc ggcggcaaat tcgaggaagg ctttaaattt
     6301 gatctccttg ttgcacagaa tatccggatt cggcgtgcga ccggctttat attcggcaag
     6361 gaacagttcg aagacgttgt cccagtactc ggcagcaaag ttaacggtgt gcagttcaat
     6421 gccgagcttg tcgcagaccg cctgggcatc agccagatcc gctgccgctg tgcaatattc
     6481 ctcaccgtcg tcttcttccc agttcttcat aaacaggcct tcgacctgat atccctgttg
     6541 ttgcaacagc caagcagaaa cggaggaatc gacaccgccg gacatgccga cgattacttt
     6601 ttttgcggtt tcagacattg gatcactcac gacattgaac ttcaaggcgg cgtattctat
     6661 cacgcagcct tttagatgac accctttgta aaaggccagt taaaatcgcc aatcatctcc
     6721 agcggataac gttgcccgct ttgataacaa cgaatacttt ccgccaccag cggcgaacga
     6781 agatttgacg cctgtaaaat ttcttcggcg ctgacccaac ggcagcagtc gatatcgctg
     6841 tcatgaggct gggtcgggca tatttgctca agctcaatgg caaagaggaa acgcaaaaac
     6901 ggcgttttat ctggcgcaat ccactgatgc atacgaataa agtgttgcgg ctgcgcgctg
     6961 atgccggttt cttcccacag ttcacgggcg gcggcttcca ctaaggtttc atcggcttcc
     7021 agatgcccgg caggttggtt ccataacgct ttaccattaa tcgtctcttc aacgactaaa
     7081 aatttgcctt ctgcgtgcac cacgcaagca acggtaacgt gcggtttaaa cattttgcat
     7141 cctcaatctg tcacttctcg ccattcgcca ttggcaagat tatccaaaga gtaatctccc
     7201 atcgcatagc gaatcagtcg cagcgtgggg aaaccaacat gggcggtcat gcggcgcacc
     7261 tggcgattac gtccttcata taaggtgatc ttcagccagc tggtgggaat acttttgcgt
     7321 tcacgaattg gtggattccg cggccataac cacgcgggtt cgtcaaccag ctccgcgcct
     7381 gcgggcaggg tagggccatc atttaaggtt acgccattgc gcaaggcttc aagtgcgtct
     7441 tgtgtgggaa taccttccac ctgcacataa tagatttttc cggtgcgttt acccggctgg
     7501 gttaaacgcg cctgcaacgc gccgttattg gtcagcacca gcaacccttc gctatcgcgg
     7561 tcaaggcgac ctgctgcata aacgccctga accgggatga attcttttaa tgttttgcgt
     7621 ccggcttcat cggtgaactg cggaagaaca tcgtagggtt tattgaagag gatcacacgc
     7681 gtgggctggt tttcatgttt acgcctggta gaacgttgcg agctgaatcg cttaacctgg
     7741 tgatttctaa aagaagtttt ttgcatggta ttttcagaga ttatgaattg ccgcattata
     7801 gcctaataac gcgcatcttt catgacggca aacaataggg tagtattgac aagtcaatta
     7861 caaatcatta acaaaaaatt gctctaaagc atccgtatcg caggacgcaa acgcatatgc
     7921 aacgtggtgg cagacgagca aaccagtagc gctcgaagga gaggtgaatg gaaagtaaag
     7981 tagttgttcc ggcacaaggc aagaagatca ccctgcaaaa cggcaaactc aacgttcctg
     8041 aaaatccgat tatcccttac attgaaggtg atggaatcgg tgtagatgta accccagcca
     8101 tgctgaaagt ggtcgacgct gcagtcgaga aagcctataa aggcgagcgt aaaatctcct
     8161 ggatggaaat ttacaccggt gaaaaatcca cacaggttta tggtcaggat gtctggctgc
     8221 ctgctgaaac ccttgatctg attcgtgaat atcgcgttgc cattaaaggc ccgctgacca
     8281 ctccggttgg tggcggtatt cgttctctca acgttgccct gcgtcaggaa ctggatctct
     8341 acatctgcct gcgtccggta cgttactatc agggcactcc aagcccggtt aaacaccctg
     8401 aactgaccga tatggttatc ttccgtgaaa actcggaaga catttatgcg ggtatcgaat
     8461 ggaaagctga ctctgccgac gccgagaaag tgattaaatt cctgcgtgaa gagatgggcg
     8521 tgaagaaaat tcgcttcccg gaacattgcg gtatcggtat taagccgtgt tctgaagaag
     8581 gcaccaaacg tctggttcgt gcagcgatcg aatacgcaat tgctaacgat cgtgactctg
     8641 tgaccctggt gcacaaaggc aacatcatga agttcaccga aggcgcgttt aaagactggg
     8701 gctaccagct ggcgcgtgaa gaatttggcg gtgaactgat cgacggcggc ccgtggctga
     8761 aagttaaaaa cccgaatacc ggcaaagaga tcgtcataaa agacgtgatt gctgatgcat
     8821 tcctgcaaca gatcctgctg cgtccggctg aatatgatgt tatcgcctgt atgaacctga
     8881 acggtgacta catttctgac gctctggcag cgcaggttgg cggtatcggt atcgcccctg
     8941 gagcaaacat cggtgacgaa tgcgccctgt ttgaagccac ccacggtact gcgccgaaat
     9001 acgccggtca ggacaaagta aaccctggct ctattattct ctccgctgag atgatgttac
     9061 gccatatggg ctggactgaa gccgcagacc tgattgttaa aggtatggaa ggcgcgatca
     9121 acgccaagac cgtaacctat gacttcgagc gtctgatgga aggcgctaaa ctgctgaaat
     9181 gttcagagtt tggtgatgcg atcatcaaga atatgtaatc actacatgtg ttaaatattt
     9241 aaacgggcgt ataacacgcc cgttgtttta tttatgtgga tattattaat agcatatcga
     9301 gcatatttat ataaaaccta ttacttgagc ccatatgggc atatttttat aatacaactc
     9361 ttatgcaaac atttatttgt tatttcgctt tctcctggag gacactcttg actgcttttg
     9421 agtaaactcc ataaattctt gttgaatggt gcaatgttat aaatagtaat aggatattct
     9481 ttttccttaa ggataatacc aagcttaacc ggtgtaaata tccttccagg aggaagaaat
     9541 atagtagatt gataccagat gatcattttc atatcacccc atatggctga aaaagatatg
     9601 ccgcatgtag gttgaattac cgtgtcaatt actgcccact tcatttgtta tgtcttatcc
     9661 cacggtattt aatatagttc atttggatgt tcatttcttt attttgcata tgagtatatt
     9721 accccttcaa aaaataaatt aattaaaacg attgcttata taaaacaaaa tttaaatcaa
     9781 ggaatctcaa tggatgttaa acaaaatgag atttagtgaa aacaataaat tattcacctc
     9841 gttttagatt tgtttagcta taatgttata cattcaaatg aatgaacatc ctgtaattaa
     9901 aacatagcct ttatgctact tggtgccaat ttgctaaaca ttatggttgc ctttttatat
     9961 aacgataata atgaatataa acatgacatg agaataaggt ttcaattttt gagttatata
    10021 ggaaaggttt aacctgttcc tggctaaaat acatataact ggatgatgac taaaccaaaa
    10081 cacatatgcg ttaagtattg aaacgggcgt atggcacacc cgttgttttt ataaatatat
    10141 taaccgttat aaaataacgt ataaaaagtc aagtgatcac atttcaaata tcaatttgat
    10201 agtatgggct tggtgattat ttatgggtag caataaaagg acagtattta tcatccatag
    10261 ggatagtctc tgtactttta ttcccattat gctaatgcct tactgaatta tgaagcattt
    10321 cttaagcatc caactttagc tagattaatg gtttattatt ttctacatct tcaatatata
    10381 aaagcgtatt atcaatggcg tagtaactgc gtttgttatg attaacatca gtaacccaac
    10441 ggaaaacgcc cgcgcctgct agtgttgaac agtattcccg aaatgtagat tttccgcaaa
    10501 tatgaagcaa tgcggcctct tttattttag caggattctt ggtcgtacta acttttaact
    10561 ggttcctggt tcctcttaat aacaaaaccg tgtcatcgtg agtaataatt ctgatgttat
    10621 ccgtagccag ataataaatg taatgtgcaa tacggtgatg ttttaattct gaataaaacc
    10681 aggagaagtt ttgctctttt ctcacttgct caaacatctt ttgaaaaaca acgacctgat
    10741 ccatcaggat aataacctct tgttagttgt gagactgcgt agtgtgcacg atcggtttta
    10801 ccacttcaat ctggtctgtc ctttggctgt gatatgtaca gagtgtgata gagggattac
    10861 ctgaattctc ccggtgagca ttttgcaacg gaccagctcc ggtacaaatg ctgttgtggg
    10921 ttcagattat aacattctgt ctaaggggcg ggataaaggt gaaattaggg ggcatgaaag
    10981 atgactttat aaccttgctc accccaatgt tgtaaaagtt cgttttgcct tctcgttggt
    11041 gccatgcctg tccagacaat caatgtttgc gtcgggaaca gttccgggcg cggcgattca
    11101 atgggatcag caagaacaga aatgtgccat cctgacaatg ataaacgcca tgcttccagc
    11161 cacaatcgtg ctctctcttc aacattccac gccatcagaa tggcttcttt accgggctta
    11221 cggcgcgttt cgaaaagcga ggttgctgcg tattcaatta atgcgccgtc aaacatactg
    11281 ctcataatgc gggaggtgtt gtgatcaagc acgagacgct ggcgaacagg aaggtaaaca
    11341 tgattaatca attgatctac tgggtactct cgacccagtg aaataattct cgcgcgtagt
    11401 ttggcaggat tagccatacg aagaattgac atcatctctt cttgcaggcg gctccagtca
    11461 tcttccgtat cctggctggt ggtttccagt aatgctttaa ctttgcctac agggacgcca
    11521 ttactgatcc aacgcttgat ctcttcgatg cgttgtatgt cttcttcatc aaagagtcgg
    11581 tgtccgcctt cactgcgctg tggttttaac aaaccgtagt ggcgttgcca ggcccggaga
    11641 gtgacaggat taatcccgca acgttcagca acatcaccaa tgctgtaata agccacaatt
    11701 cctccttgcg gtcacaaatc tccgtcgcct gtacacgacc caataatact ttgtacaata
    11761 tacgctaaaa ttgtacaaag tataaataag atttcagcta aattggatga aacattattt
    11821 ttaatgtaga ttaaatttaa acataacgta ttcattttca cgatgattta ctgaaatcat
    11881 gtgaaagaat gtgctgaaaa ttattttttc tctggccacg ctatggaagg gataccattc
    11941 aatttagctt tagcaaacag atctccctga aacatctcaa ttcctgcgga ttcaagccac
    12001 atccactctt ctggtgttgc cacgcccata gcactgactt gaatttcaag tgatgtacag
    12061 cattttatga tcgcttgaat aattgcctgc cgtggcccgc ttttatgaac attggtaatc
    12121 aattcctgac tgattttaat tctgtcaggc tggaagcgtg acaggagtaa caaaccggca
    12181 aaacctgcgc caaaatgatc aattgctaca ctgataccgg cagccttaag cgatttaatc
    12241 gcttctgcaa actcatcaaa ccgagatata acttcacttt cagtaaattc aacgatgatt
    12301 tgttcaggca ccagagcatt ggcctttatt tcatcaagta aaaaagagac tgcgtcaggt
    12361 tcgttaacca gggtcatagg taatagattg attgaaatca ttttatcacc gagctcaagt
    12421 gcgtgtgtca tcgcgaatgc aagcgcctta cttttgagat ccgctgtgta gatttccccg
    12481 tctttacgct gcccaaccgc tatggctgat gggctatctt catttttttg cacaatggct
    12541 tcaaaagcga ttatccgccg cgataaggga tcgacaatag gatgaaaggc aaagtggtcg
    12601 tttatagttg gtgaaagggt acaggaatca agttctttat cagatccgtc agcgataaaa
    12661 agccaggagt cttcggcagg gatctcgaaa taggttgatt gttcggttgc aaggacaaaa
    12721 gtacgaaaaa attgtagcgc tctgtcatca taagttagct gaaattttga tgtgcctttg
    12781 tcgaatacgg cctgtaaaac gtcatctcgc tcgtgcaggc gcaaatcaaa taattccatt
    12841 cccgctttgc caaaacggcg agcaggcgcg taatcgcaca gcagctcaac aatattatag
    12901 tgccgtggat cttggcatat agcccgatat atcattttaa cctgttcttc cggaccttcc
    12961 agaagctgga aaaaatgaga accattaaac agtaagatcc ctgttacgtc agactgcatg
    13021 ttcctgcgat ttgctatcga aaccatttct tcgatttttt tgacaggttc gtcgtcacgt
    13081 atatggctac gataaataag ggtggtaagc attaacaatc cagggtaatg ggtgaggcga
    13141 gagtaagacg gtaacagaca tatcttcttg tgtctttctt ttaataccaa aacataaccg
    13201 tttctttaca ttgataaaaa atggaaaaag ttgaacacga gttggcgaaa aatcttgtat
    13261 agattgtcag ttaaatgatg caatatgttt tatcataaca cattgtttta tatacattag
    13321 tactaattgc aaaaaattaa tttatcattc tgtacacata tttcgtacaa gtttgctatt
    13381 gttacctcac ttcacattga ttaacatttt taacagaggc gtagcatgca tcaaaattca
    13441 gtgacttcag attctgcggg agcaatcact cgttactttg caaaagctaa cttgcctact
    13501 cagcaggaaa ctctcgggga aattgtgact gagattttga aagatggacg taatctgagt
    13561 cgaaagtcgc attgtgccaa acttctttgc cgactggaac aagcgacagg ggaagaggaa
    13621 cagaaacatt ataatgcact aattgggttg ctttttgaat aatgcaacag cctgattatt
    13681 taacaggtta gctatgaagt cgttatgaag acatctgata atgaacgtat aaaatatgaa
    13741 attactggcc aggcggtgct ccagatactg cgcatgaaga taaatttttc attgcagact
    13801 cttattaagc aattactcgt aatgaaattg gctgaggaag atgctttccg acgcgattta
    13861 attgacagca taattcgtga ttttagcaat agtgattcag gagggccaaa ccgaagaaca
    13921 gcgacagctg acaataaaag tatgttcaat ggtaagaaaa taaacagaat acattaaaat
    13981 ttcataagta agatgagagg ttaccatgct tgaagatact acaattcata atgcaataac
    14041 tgataaagcg ttagcaagtt actttcgcag ttcgggtaat ttgttagaag aagagtcagc
    14101 agtgttaggg caggctgtca ccaatttaat gctttcaggc gataatgtta ataataaaaa
    14161 tattatctta agtctgatac actccctgga aacaacaagt gatattctca aagctgatgt
    14221 gattagaaaa acactggaaa tcgtgttgcg atacacagct gatgatatgt aacttctaat
    14281 tattaagtat aagtttatag aaaactcatt catcttattt ttgtctgtcg ctttagacta
    14341 tacacagcat aattttattg ggttaatatt tctacgaggc tgacatgaat aattcaatcc
    14401 cagagagatt tatttttcaa tgtgctttgt ttaaaaatct cgagagggag gtgttcatga
    14461 cgcatgggta tgttgatagt catattattg atcaggcttt acgcttgcgt ttaaaagatg
    14521 aaaccagtgt gattctttct gatctctatc tgcaaatatt gcagtacatt gaaatgcata
    14581 agactacact aacggatatc attattaatg acagggaatc cgtgctctct tagaacttga
    14641 gtaaggtttt agaataaaca tcagctgtat caccatgctg atgcaaagtg agcatttcag
    14701 gcgttatgct ttcttattat gtccgcaata tcaggtgtca agaatggaga gttctcgctc
    14761 tccattcttg acgcctgata tcccgcctaa cttattttgt actgcctaca ttatgctcaa
    14821 taattaatgg gttagatcaa gatggagaaa attttatctt cggcgtggat atttcttatg
    14881 ctctctacca tgcatctctg tagatgccag cgcgagcgaa tgaatgg
//
LOCUS       DAETPZ010000090        14687 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.090, whole genome shotgun sequence.
ACCESSION   DAETPZ010000090 DAETPZ010000000
VERSION     DAETPZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.090"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            118..1419
                     /gene="rstB"
                     /locus_tag="K0W59_003192"
     CDS             118..1419
                     /gene="rstB"
                     /locus_tag="K0W59_003192"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HBI3024126.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            1495..2424
                     /gene="tus"
                     /locus_tag="K0W59_003193"
     CDS             1495..2424
                     /gene="tus"
                     /locus_tag="K0W59_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HBI3024127.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHEPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(2421..3824)
                     /gene="fumC"
                     /locus_tag="K0W59_003194"
     CDS             complement(2421..3824)
                     /gene="fumC"
                     /locus_tag="K0W59_003194"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HBI3024128.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSIKAGR"
     gene            complement(3967..5613)
                     /gene="fumB"
                     /locus_tag="K0W59_003195"
     CDS             complement(3967..5613)
                     /gene="fumB"
                     /locus_tag="K0W59_003195"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBI3024129.1"
                     /translation="MSNKPFHYQSPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            5812..6987
                     /gene="manA"
                     /locus_tag="K0W59_003196"
     CDS             5812..6987
                     /gene="manA"
                     /locus_tag="K0W59_003196"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HBI3024130.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIKSDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELGFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            7088..8596
                     /locus_tag="K0W59_003197"
     CDS             7088..8596
                     /locus_tag="K0W59_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBI3024131.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8822..10087)
                     /gene="uidC"
                     /locus_tag="K0W59_003198"
     CDS             complement(8822..10087)
                     /gene="uidC"
                     /locus_tag="K0W59_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HBI3024132.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYVAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(10126..10293)
                     /locus_tag="K0W59_003199"
     CDS             complement(10126..10293)
                     /locus_tag="K0W59_003199"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HBI3024133.1"
                     /translation="MGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKK
                     VQQQLISDITN"
     gene            complement(10309..11499)
                     /gene="uidB"
                     /locus_tag="K0W59_003200"
     CDS             complement(10309..11499)
                     /gene="uidB"
                     /locus_tag="K0W59_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HBI3024134.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQV"
     gene            complement(11496..13307)
                     /gene="uidA"
                     /locus_tag="K0W59_003201"
     CDS             complement(11496..13307)
                     /gene="uidA"
                     /locus_tag="K0W59_003201"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBI3024135.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHGFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(13695..14285)
                     /gene="uidR"
                     /locus_tag="K0W59_003202"
     CDS             complement(13695..14285)
                     /gene="uidR"
                     /locus_tag="K0W59_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HBI3024136.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(14514..>14687)
                     /locus_tag="K0W59_003203"
     CDS             complement(14514..>14687)
                     /locus_tag="K0W59_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI3024137.1"
                     /translation="WSHYPEIEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQ
                     ILTVSGGGVQELN"
BASE COUNT         3685 a         3819 c         3498 g         3685 t
ORIGIN      
        1 ggatacatcc gtctgttccg gacattatct tccaaacaag gtcttatcca gttggcggtt
       61 tacattgctc cacagtcgtg aattaatacg aatagtattc tgttgagggc gatgccaatg
      121 aaaaaattgt ttatccagtt ttacctgtta ttgtttgtct gcttccttgt gatgtctctg
      181 ctggttgggc tggtgtacaa atttaccgcc gaacgcgcgg gcaaacagtc gctggatgat
      241 ttgatgaaca gttcgctgta tctgatgcgc agcgaattgc gagagatccc cccacacgac
      301 tggggtaaga cgctgaaaga gatggattta aatctctctt tcgatctgcg tgtcgagcca
      361 ctgagtaaat accatcttga tgatatttcc atgcaccgac tgcgtggcgg cgaaattgtc
      421 gccctggacg atcagtacac gtttttgcag cgtatcccgc gcagccacta cgtgctggca
      481 gttggtcctg ttccttatct ttattacctc catcaaatgc gattgctgga tatcgccctg
      541 atcgctttta ttgctatttc cctcgccttt ccggtgttta tctggatgcg tccgcactgg
      601 caggatatgt taaaactgga agcagcggcg caacgatttg gcgatgggca tctcaatgaa
      661 cgtatccact ttgatgaggg ttcgagcttt gaacgacttg gtatcgcatt taaccagatg
      721 gcggacaata tcaacgcctt aattgccagc aaaaaacagc ttattgacgg tatcgctcac
      781 gaactgcgaa caccgttagt gcgcctgcgt tatcgactgg agatgagcga taacctgagc
      841 gccgccgaat cccaggcgtt gaatcgtgat atcagtcaac ttgaagcttt aattgaagag
      901 ctgctgactt atgcccgact cgatcgccca caaaacgagc ttcatcttag cgaaccagac
      961 ctgccgttgt ggctgtcaac gcatctggca gatattcagg cagtaacgcc cgataaaacg
     1021 gtacggataa aaacgctcat gcaaggccat tatgcggcgt tggatatgcg cttaatggag
     1081 cgcgtgctgg ataatttgct caataacgcc ctgcgctact gccattcaac ggttgaaacc
     1141 agcctgctac tgtcggggaa tagagcgaca ttaattgttg aggatgatgg cccagggatt
     1201 gccccagaaa accgcgaaca tatctttgaa ccttttgttc gcctcgatcc cagccgggat
     1261 cgctcaaccg gcggctgcgg gctggggctg gcaattgtcc actctatagc actggcaatg
     1321 ggcggtacgg ttaattgtga catcagcgaa ctgggtggtg ctcgcttctc gtttagctgg
     1381 ccgttatggc ataacatccc gcaatttacc tctgcctgac actacgcgca cgatggtcaa
     1441 gtcacaacga ctgtgctata aaataagtat gttgtaacta aagtggttaa tattatggcg
     1501 cgttacgatc tcgtagaccg actcaacact acctttcgcc agatggaaca agagctggct
     1561 acatttgccg ctcatcttga gcaacacaag ctattggttg cccgcgtgtt ctctttgccg
     1621 gaggtaaaaa aagaggatga gcataatccg cttaatcgta ttgaggtaaa acaacatctc
     1681 ggcaacgacg cgcagtcgct ggcgttgcgt catttccgcc atttatttat tcaacaacag
     1741 tccgaaaatc gcagcagcaa ggccgctgtc cgtctgcctg gcgtgttgtg ttaccaggtc
     1801 gataaccttt cgcaagcagc gttggtcagt catattcagc acatcaataa actcaagacc
     1861 acgttcgagc atatcgtcac ggttgaatca gaactcccca ccgcggcacg ttttgaatgg
     1921 gtgcatcgtc atttgccggg gctgatcacc cttaatgctt accgcacgct caccgttctg
     1981 cacgaacccg ccactttacg ctttggttgg gctaataaac atatcattaa gaatttgcat
     2041 cgtgatgaag tcctggcaca gctggaaaaa agcctgaaat caccacgcag tgtcgcaccg
     2101 tggacgcgcg aggagtggca aagaaaactg gagcgagagt atcaggatat cgctgccctg
     2161 ccacagaacg cgaagttaaa aatcaaacgt ccagtgaagg tgcagccgat tgcccgcgtc
     2221 tggtacaaag gagatcaaaa acaagtccaa cacgcctgcc ctacaccact gattgcactg
     2281 attaatcggg ataatggcgc gggcgtgccg gacgttggtg agttgttaaa ttacgatgcc
     2341 gacaatgtgc agcaccgtta taaacctcaa gcgcagccgc ttcgtttgat cattccacgg
     2401 ctgcacctgt atgttgcaga ttaacgcccg gcttttatac tgccgaccat ctgttctggc
     2461 cgtacccagc tgtcaaactc ggcttcgcta agatacccca gcgcaagggc cgcagctttt
     2521 aaggtcagcc cttctttatg cgcttttttg gcgatctccg cggctttgtc ataaccaatg
     2581 tgggtgttaa gcgcagtcac cagcatcagc gattcattga gtaattgatt gattcgctca
     2641 cgattcggtt caatacccac tgcgcagtgt ttgttaaaac tttccatgcc atctgccagc
     2701 aagcgcaccg attgcaggaa attatggatc accatcggac ggaagacgtt cagttcaaag
     2761 ttaccggaag cgccacccat gttgatcgcc acgtcgttcc ccattacctg acagcagagc
     2821 atggttaatg cttcacactg ggttgggttc actttccccg gcatgattga gctgcccggc
     2881 tcattttccg ggattgagat ttcaccaatt ccgcagcgcg ggccagaggc cagccagcgg
     2941 acatcattgg cgattttcat cagtgacgca gccaaccctt tcaacgcgcc gtgcgcctga
     3001 accagggcat cacaggtcgc aagcgcttca aatttgttcg gcgcggtaac aaacggtgca
     3061 caggtaatga ctgccagttc atctgctacg cgccgcgcat actccggatg ggtatttagt
     3121 ccagtaccca ccgctgtacc gccaagagcc agttccgcta cgtgaggcag gctgtattcg
     3181 atatgtttga gattatgctc gagcatcgct acccagccgg aaatctcctg ccctagtgtt
     3241 agcggcgtgg cgtcctgcaa gtgggttcga ccgattttga cgatatcggc aaatgcacgc
     3301 gatttttcac tcagcgtctg tgtcagggtt ttaagctgcg gaatgagttg cttgcgcagt
     3361 gccagcagcg ccgcaacgtg catcgccgtc ggaaagacat cgttggaact ttggcttttg
     3421 ttcacgtcgt cgttagggtg aactttacgt tccatcccgc gcacaccgcc gagtaattca
     3481 ctggcccggt tagccagcac ttcgttcatg ttcatgttac tttgcgtgcc ggagccggtc
     3541 tgccagatag ccagcgggaa ttcgtcgtca tgctgtcctg ccagtacttc atccgccgcc
     3601 tgccgaatgg cgctcgcttt ctcttcagac aacaagtcta aatcttcatt aacttttgcc
     3661 gctgcgcgct tggttagcgc cagcgcatga atcagtgagg tgggcatttt ctccgtcgaa
     3721 atgcggaaat gctccagcga gcgttgagtt tgtgcgcccc acagcttatc tgccgggaca
     3781 tcaatcgccc ccatcgaatc tttttcgctg cgtactgtat tcatggcctg ctcctcacct
     3841 gattaatttt ttctttctgt tttgctttcg ttaagcaact tttagctcac tcattattta
     3901 ctatttgata acaaatgttt ggtctttcgt gccatgtaaa aaaaccgccc cgaagggcgg
     3961 ctctgtttat ttcacacagc gagtgcactg tgtgagttgt atctgctgga agaagtcatt
     4021 tcctttatca tccacaagga taaacgccgg gaaatcttcc acttcaattt tccagatggc
     4081 ttccattccc agttccggat attcaacaca ttccaggctc ttaatacttc cctgcgccaa
     4141 tacagcggcc ggaccaccga tactgccaag gtagaagccg ccgtgttttt tacaggcatc
     4201 cgtcacctgc tggctgcggt tgcctttcgc cagcatgatc atacttccgc cctgcgcttg
     4261 cagttgatcg acataagaat ccatccgtcc ggcggtcgtt gggccaagag aaccggaggc
     4321 ataaccttcc ggcgttttgg ccggacccgc gtagtaaatc ggatgatctt taatgtattg
     4381 cggcagccct tcaccgttat ccatccgctc tttcagtttg gcgtgagcaa tatcacgacc
     4441 aacgataatc gtgccgttaa gcgataagcg tgtagaaacg ggatactgcg acaactgtgc
     4501 gaggatctct ttcatcggac ggttaaggtc aacgcgcacc gcttcgcctt ctcccgcttt
     4561 acgcagctct tcagggatat atttacctgg attatgttcc agtttttcga tccagatccc
     4621 ctgacggttg atcttcgctt tgatattacg gtcagcagag caggaaacac ccataccaac
     4681 cggacaggat gcgccgtgac gtggcaggcg aatcacgcgg atgtcgtgag cgaagtattt
     4741 accaccaaac tgcgcaccca gaccgagatt ttgcgcttcg atcagtaatt ctttttccag
     4801 ttccacatcg cggaacgcct gaccgtgctc attcccttcc gttggcagtt catcatagta
     4861 tttcgcggaa gccagtttca ccgttttaag gttcgtttct gcagaagttc caccaataac
     4921 gaacgcaata tgatacggag gacaggccgc ggtacccagc gtgcgcatct tctcaaccag
     4981 gtaatttttc agtttccccg gcgtcagtaa cgctttggtt tcctgataga gatacgtctt
     5041 gtttgccgaa ccaccacctt ttgcgataca gaggaatttg tattcgtcgc catcaacggc
     5101 ataaagatcg atctgcgctg gcagattggt gccggtattc acttctttat acatatccag
     5161 cggcgcgttt tgcgagtagc gcagattatc ttcgatataa gtgttataga caccgcgcgc
     5221 cagcgccgct tcatcaccac caccggtcca tacacgctgc ccttttttac caacaataat
     5281 cgcggtgccg gtatcctgac aggttggcag aacgcctttc gccgcgatgt cggagttacg
     5341 caggaattgc agcgccacat atttatcatt ttcgctggcc tccgggtcac gcagaatgtc
     5401 ggccacctgt tgttggtgag ccggacgcag cataaacgac gcatcatgaa acgcctgacg
     5461 cgccaacaga gttaacgctt cgggtgcgac tttcaaaatc tcctgccctt caaattcaga
     5521 tacgctaacg tgttcactgg ttagcaggta atactcagta tcatcttttt tgagtggaaa
     5581 aggagactga taatgaaagg gtttgtttga cattgttctc tcacttactg cctggtttgg
     5641 ttatgctctg ggcgggtgtt ccgttgccct gttaaaagcg agtaacaata tcctacacag
     5701 ttttttaaca aaaactgaga ctagtacgac tttttgcggc tccaggttac ttcccgtagg
     5761 attcttgctt taatagcggg attaatttcc acattaaaac agggattgat catgcaaaaa
     5821 ctcattaact cagtgcaaaa ctatgcctgg ggtagcaaaa cggcgttgac tgaactttat
     5881 ggtatggaaa atccgtccag ccagccgatg gccgagctgt ggatgggcgc acatccgaaa
     5941 agcagttcac gagtgcagaa tgccgccgga gatatcgttt cactgcgtga tgtgattaag
     6001 agtgataaat cgactctgct cggagaggcc gttgccaaac gctttggcga actgcctttc
     6061 ctgttcaaag tattatgcgc agcacagcca ctctccattc aggttcatcc aaacaaacac
     6121 aattctgaaa tcggttttgc caaagaaaat gccgcaggta tcccgatgga tgccgccgag
     6181 cgtaactata aagatcctaa ccacaagccg gagctggttt ttgcgctgac gcctttcctt
     6241 gcgatgaacg cgtttcgtga attttccgag attgtctccc tactccagcc ggtcgccggt
     6301 gcacatccgg cgattgctca ctttttacaa cagccacatg ccgaacgttt aagcgaactg
     6361 ttcgccaacc tgttgaatat gcagggtgaa gaaaaatccc gcgcgctggc gattttaaaa
     6421 tcggcgctcg acagtcagca gggtgaaccg tggcaaacga ttcgtttaat ttctgaattt
     6481 tacccggaag acagcggcct gttctccccg ctgttactga atgtggtgaa attgaatcct
     6541 ggcgaagcga tgttcctgtt cgctgaaaca ccgcacgctt acctgcaagg cgtggcgctg
     6601 gaagtgatgg cgaactccga taacgtgctg cgtgcaggtc tgacgcctaa atacattgat
     6661 attccggaac tggttgccaa tgtgaaattc gaagccaaac cggctaacca gttgttgacc
     6721 cagccggtga aacaaggtgc agaactgggc ttcccgattc ctgtggatga ttttgccttc
     6781 tcgctgcatg accttagtga taaagaaacc accatcagcc agcagagtgc cgccattttg
     6841 ttctgcgtcg aaggcgatgc aacgttgtgg aaaggttctc agcagttaca gcttaaaccg
     6901 ggtgaatcag cgtttattgc cgccaacgaa tcaccggtga ctgtcaaagg ccacggccgt
     6961 ttagcgcgtg tttacaacaa gctgtaagag cttactgaaa aaattaacat ctcttgctaa
     7021 gcttattaaa ggcttataac accttcaggc ggccagtccg cctgatttca ttttatggat
     7081 aatcattatg aataaatcgc tggtagcggt aggcgtcatt gttgcgctag gcgtagtctg
     7141 gacaggcggc gcatggtata caggcaagaa gattgaaacc catctcgaag acatggtcgc
     7201 gcaggcgaac gcgcaactca aactgacagc tcctgaatcc aacctggaag tgagttatca
     7261 aaactatcat cgcggcgtat tcagcagcca gttgcaactg ttggtgaaac ccattgccgg
     7321 gaaagaaaat ccgtggatta aaagcggtca gagcgtcatc ttcaacgaat cggttgatca
     7381 tggtcccttc ccgcttgccc agcttaaaaa actgaacctg atcccgtcga tggcatcaat
     7441 tcaaaccacg ctggttaata acgaagtaag caaaccactg tttgatatgg caaaaggtga
     7501 aacgcctttt gagattaact cgcgcattgg ttacagcggt gattccagtt ccgatatttc
     7561 gctcaatcca ctgaattacg agcaaaagga tgaaaaagtc gcctttagcg gcggcgagtt
     7621 ccagttaaat gctgacagag acggcaaagc catctccctt tccggggagg cgcaaagtgg
     7681 tcggatagac gcagttaacg aatacaacca gaaagtgcag ttgaccttta ataatctgaa
     7741 aaccgacggt tccagcacgc tgggaagttt tggtgagcgc gtaggaaacc aaaaactgtc
     7801 actggaaaaa atgaccattt cagtggaagg caaagaactg gcactgctgg aaggcatgga
     7861 gatcagcggt aaatcggatc tggtcaatga cggtaaaacg atcaatagcc aactggatta
     7921 ctcgctaaac agcctgaagg tacagaatca ggatctgggc agcggcaagc tgactttaaa
     7981 agtcggccag attgatggtg aagcctggca tcagtttagc cagcaatata acgcgcaaac
     8041 tcaggcgctg ctggcacagc cagaaattgc caacaatccc gaactttatc aggagaaagt
     8101 gacggaagcc ttctttagcg ccctgccgct gatgttgaaa ggcgatccgg tgattactat
     8161 cgcgccgcta agctggaaaa acagtcaggg tgaaagtgcg ctgaatctgt cgctgttcct
     8221 gaaagatccg gcaacgacta aagaagcgcc gcaaacgctg gcgcaggaag tagatcgttc
     8281 ggttaaatct ctggatgcga aactgaccat tccggtggat atggcaactg agttgatgac
     8341 tcaggtagcg aagctggaag gttatcagga agatcaagcg aaaaaactgg cgaaacagca
     8401 agttgaaggt gcatcagcaa tggggcagat gttccgtctg accaccttgc aggacaatac
     8461 catcaccacc agcctgcaat atgctaacgg tcagataacg ttaaatgggc agaaaatgtc
     8521 gttagaagat tttgttggca tgtttgcgat gccagctctt aacgttccgg ctgtaccggc
     8581 aattccgcag cagtaattca cggccaaaac gccggagaat tttcactccg gcgttttcat
     8641 ctgaaaacaa accagagatt gcctgatgcg ctacgcttat caggcctatc ttttcctgca
     8701 acactttgaa tttatgagtt tttgtaggct ggataaggcg tttacgccgc atccggcata
     8761 aaaaacgcgc actttgtcaa caatctgaaa cgccggaggt ttttctctcc ggcgttattt
     8821 cttacttcag cataaagtca tactgaatac tcattttcac atcccagtaa ttcaggcgat
     8881 cgcccagcgt actgccatcg cgcttttccc gccctttatc ggcagcaaga aagaaattca
     8941 tcccttgtaa cggcccttcg ctgacccgat agttcatacc acctaagata cccgcgcctt
     9001 caatccgcac atctcgttta ctggattcat agccgcgagt cacaatcggt gcaaatgtca
     9061 gcgtaaattg cggagttaca cggtagttga cacctaattg ccaggactgc attccttcac
     9121 ggttacgatc aagacttaat gaaaagggat agccaatgtc ggtatccacc acggcaccat
     9181 tcattgcatg gcgcaaataa ccaccactgc caaatacgct tccccacggc gcattccagg
     9241 tcaattgtcc actaaccaac gccgtttcat tgggctggct ggtattacga cttaaacctt
     9301 cgagctgcgc gtaaaagccg agcaactccc ctttcaacgt ataattcggg tcgagcggta
     9361 tgcttttctc gagaatactt tgtatctgat tggaaacatc tttctgtacc gatgccccca
     9421 gagaatagcg actggtatca tcatgccaac tggcggctaa aaaagaacgt gcgcgatgtt
     9481 tatcgtattt tcccgtctgg ctgttataca ccccttcatc acgaataccg gttgttgaat
     9541 cagtccataa aaatactcgt gaacgccaca ttccccacag atcaatattt ggtgttaaag
     9601 cccaataacc taatgctcct tcagaaactg tcggtaatgt acgttgcgaa ctgccagcca
     9661 ttaacggaat acgataaggt aagctaccat aactataatc cgtcccgaag cgacctaatt
     9721 taagcaggct attttcactg ggttttattt ttactgcccc cagggcaaag ccaaaacttt
     9781 tatcaccatc aagataccac cgggtactca tatcagcacg agcacctaat ttataaacat
     9841 aatacgctcc gccttcaacg ccaataatat tggaataata accactattg aaatcgagca
     9901 atcctccctg tacccatgcg taaatatctc ttcccgcgcc agcactgggc ttatcagccc
     9961 tgcgcaattc gtttttcagt ctgatgcgta gtcccgcttc atcgtcagcc agttgcgcca
    10021 cgtaagcaga ggtaaggcca gaggcagccg tcaggcaaat aacggccatg gccactattt
    10081 ttctcatagt tgcacctttg atgttctgat tatttttatt gaatattaat tagtgatatc
    10141 gctgattaat tgctgctgca cttttttacg attatcaatt tcaaccacga tttctttgaa
    10201 ttttttatcc gtgagcggat aaaaccagat aataacgaat gccagtagca taaatccgca
    10261 aggtactaag gcaattgatg tgcggatgcc cataataact tccggcgttt acacctgatt
    10321 ggcgatatat ccgcttaatc ccaaaataaa ggcaggaatt gaacctccga ttgcctgacc
    10381 gcatttacgg gtaaatgaga atagtgaata ggtgagccct tcaattcgca cgccggtcag
    10441 gtattcaccg tattctacgg tatcagcttc cagtgcccac atcacagtca tagtaacgcc
    10501 ctgaccaatt gaagcgatgg ccaacgcaac caacgccacc ggcagtgacc agacggaaac
    10561 ccagaagaat agcagataac cgcaggttcc cagcaaagcg ccaatcagga aggtattctt
    10621 tttaccgatc ctcgcgacca tccccggcac aagcggtgcc gatgccacag taccaaccag
    10681 gttttgcacc agtaccagca cagtgaacag cccggtatca tttaacacat agcgcacgta
    10741 gaacaacgac gaggcgctga ccgcgaaggt cgaaatcagc acacacagcg caccgatgca
    10801 caacataaac agcgggcgat tccgtttcag ggtttgcaaa ctgatcttca atgacggctg
    10861 cgccacgata cgtaccacat tttcacgcgt cgatttgaag cagatgaagt aaagcaccat
    10921 tccggcaatc gccagcacaa tcgtccagaa atggtatacc gacaccatct cttccgggct
    10981 ggagttctta atgctcggtc ctatcagaaa tgccaggcat acaaaggtca atgaagcggc
    11041 aatcccacga gccgcgccca gacgggcgcg agattgtggt tgttgggtca tcgcggtagc
    11101 aagtgaacca taaggaatat tcaccaggct gtagcaaagc ccgaggccca tgtaggtcaa
    11161 atatgcatac accactttgc taccatggct ccagtcggtc ggtacccaga ataccagcac
    11221 gctgaagatc attaacggcg cagtaccgaa gagtaaaaac gggcggaatt ttccccagcg
    11281 ggtattcaca ctgtccacca ctcgtccggc aaagacgtcg gcgaaggcat cgaatacccg
    11341 caccagtaac agcatggtgc ctgccgcagc ggcaccgacg ccagcgacgt cggtgtagta
    11401 actcaatagg aagagcgccc ccattgcgaa ggcgaagtta ttggcgacgt caccgaggct
    11461 gtagccgacg atggtgcgcc aggagagttg ttgattcatt gtttgcctcc ctgctgcggt
    11521 ttttcaccga agttcatgcc agtccagcgt ttttgcagca gaaaagccgc cgacttcggt
    11581 ttgcggtcgc gagtgaagat ccctttcttg ttaccgccaa cgcgcaatat gccttgcgag
    11641 gtcgcaaaat cggcgaaatt ccatacctgt tcaccgacga cggcgctgac gcgatcaaag
    11701 acgcggtgat acatatccag ccatgcacac tgatactctt cactccacat gtcggtgtac
    11761 attgagtgca ggccggctaa cgtatccacg ccgtattcgg tgatgataat cggctgatgc
    11821 agtttctcct gccaggccag cagttctttt tccagtacct tctctgccgt ttccaaatcg
    11881 ccgctttgga cataccaacc gtaataacgg ttcaggcaca gcacatcaaa gagatcgctg
    11941 atggtatcgg tgtgagcgtc gcagaacatt acattgacgc aggtgatcgg acgcgtcggg
    12001 tcgagtttac gcgttgcttc cgccagtggc gcgaaatatt cccgtgcacc ttgcggacgg
    12061 gtatccggtt cgttggcaat actccacatc accacgcttg ggtggttttt gtcacgcgct
    12121 atcagctctt taatcgcctg taagtgcgcc tgctgagttt ccccgttgac agcctcttcg
    12181 ctgtacagtt ctttcggctt gttgcccgct tcgaaaccaa tgcctaaaga gaggttaaag
    12241 ccgacagctg cagtttcatc aatcaccacg atgccatgtt catctgccca gtcgagcatc
    12301 tcttcagcgt aagggtaatg cgaggtacgg taggagttgg ccccaatcca gtccattaat
    12361 gcgtgatcgt gcaccatcag cacgttatcg aatcctttgc ctcgcaaatc cgcatcttca
    12421 tgacgaccaa agccagtaaa gtagaacggt ttgtggttaa tcaggaactg ttcgcccttc
    12481 actgccactg accggatgcc gacgcgcagc gggtagatat cacactctgt ctggcttttg
    12541 gctgtgacgc acagttcata gagataacct tcacccggtt gccagaggtg cggattcacc
    12601 acttgcaaag tcccgctggt gccttgtcca gttgcaacca cctgttgatc cgcatcacgc
    12661 agttcaacgc tgatatcacc attggccacc acctgccagt caacagacgc gtggttacag
    12721 tcttgcgcga catgcgtcac cacggtgata tcgtccaccc aggtgttcgg cgtggtgtag
    12781 agcattacgc tgcgatggat cccggcatag ttaaagaaac catggaagta agactgcttt
    12841 ttcttgccgt tttcgtcggt aatcaccatt cccggcggga tagtctgcca gttcagttcg
    12901 ttgttcacac aaacggtgat acgtacactt ttcccggcaa taacatacgg cgtgacatcg
    12961 gcttcaaatg gcgtatagcc gccctgatgc tccatcactt cctgattatt gacccacact
    13021 ttgccgtaat gagtgaccgc atcgaaacgc agcacgatac gctggcctgc ccaacctttc
    13081 ggtataaaga cttcgcgctg ataccagacg ttgcccacat aattacgaat atctgcatcg
    13141 gcgaactgat cgttaaaact gcctggcact gcaattgccc ggctttcttg taacgcgctt
    13201 tcccaccaac gctgatcaat tccacagttt tcgcgatcca gactgaatgc ccacaggccg
    13261 tcgagttttt tgatttcacg ggttggggtt tctacaggac gtaacataag ggactcctca
    13321 ttaagataaa aatactggtc aacctttaat ctgattagtt aaccaattct gtgttgcaca
    13381 cagttatctg tgagaaaccc atggaagaga aatggaaata aaaagaactg acaccgcgat
    13441 caaaaaacca aaaactgcaa tacccacagc gtgatatgga tcgcattaat ctttaaaaca
    13501 gcgttgacag aagccataaa acaagcaatc gagcgttcgt tcgagagtat tgggaacgat
    13561 aaactctcat gacatatggc tacggtgaat attttggggg ggaattctcc gaacacgggg
    13621 aggataattt ttgaacagag aaattacgat atgaaacgcg ggcacagtta tcaccagccc
    13681 gcgaaatgag aggatcagga tgcggttaat ataccgccaa tcatcgcgcg taatccctga
    13741 gcaaacgacg cttcacgcgc caggtcttcc gcttcgatat cggccagtac accgtaggtc
    13801 agatccagca gtaaacgtga agtcattgcc gtgttgatgt ctgggcttat ctcgcctttt
    13861 tgctgggcat cacgcatccg ctgggcaaca aattcagtga tcgtcatatg cttattttta
    13921 agcatggcgg cgacctgtgg gttacgcatc ccttccgcca taatttcgac caccagcgcc
    13981 cgttgcccaa aggcttcatg ggtgagagag acaatagact cgaccatata gtcaacaaaa
    14041 tgaatccctt caatcggttc ccggaaacga gccagcgccc tctcctggtc ctgtaagata
    14101 atcgcctgaa tcaaggcttc tttggagatg aaatggtgat agagcgtccc aggactaatg
    14161 gcgcaagatt tacagatggc tttcatcgag gcactgtgaa atccattttc tgaaaaaatc
    14221 tctctggcag cattgaggat ccgggtccgt gtcggttgtg cttcagtctg catgttatcc
    14281 atcattaaac cattatagtt agcgtgaata actagtttac cgcgttgtca ggtgagcgcc
    14341 tatcattgct gttgaatagt gcgaaggtac actctattcg tatacttttc acccagcaat
    14401 tggttaacca attgccgtga tttggctgcc agttgtttag cagagacgtt gatgaattgc
    14461 acacgctatc ccggtaaaca acacatacga ccgtttaccg gttcgttagt gtattaattg
    14521 agctcctgta ccccaccacc ggagacggtg agaatttgtc cgcttaccca gctggctgca
    14581 ggcgagcaaa ggaacagcgc cgcgttagca atatcttgcg gttggcccag acgtctgatc
    14641 ggcgtgtgct gtaacatttt ttgttcaatt tctgggtaat gactcca
//
LOCUS       DAETPZ010000091        14527 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.091, whole genome shotgun sequence.
ACCESSION   DAETPZ010000091 DAETPZ010000000
VERSION     DAETPZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.091"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(337..582)
                     /locus_tag="K0W59_003204"
     CDS             complement(337..582)
                     /locus_tag="K0W59_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024138.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1227..1302)
                     /locus_tag="K0W59_003205"
     tRNA            complement(1227..1302)
                     /locus_tag="K0W59_003205"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Phe,seq:gaa)
     gene            complement(1408..2115)
                     /locus_tag="K0W59_003206"
     CDS             complement(1408..2115)
                     /locus_tag="K0W59_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBI3024139.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAIPLSAAWTAWFA"
     gene            2513..4648
                     /gene="speC"
                     /locus_tag="K0W59_003207"
     CDS             2513..4648
                     /gene="speC"
                     /locus_tag="K0W59_003207"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBI3024140.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADISPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="K0W59_003208"
     CDS             complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="K0W59_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HBI3024141.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGTFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="K0W59_003209"
     CDS             complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="K0W59_003209"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HBI3024142.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7299..7574)
                     /locus_tag="K0W59_003210"
     CDS             complement(7299..7574)
                     /locus_tag="K0W59_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HBI3024143.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="K0W59_003211"
     CDS             complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="K0W59_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBI3024144.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAPLPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8815..9534
                     /gene="trmB"
                     /locus_tag="K0W59_003212"
     CDS             8815..9534
                     /gene="trmB"
                     /locus_tag="K0W59_003212"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBI3024145.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9534..9860
                     /locus_tag="K0W59_003213"
     CDS             9534..9860
                     /locus_tag="K0W59_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HBI3024146.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10044..10763
                     /locus_tag="K0W59_003214"
     CDS             10044..10763
                     /locus_tag="K0W59_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HBI3024147.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            10939..11985
                     /gene="ansB"
                     /locus_tag="K0W59_003215"
     CDS             10939..11985
                     /gene="ansB"
                     /locus_tag="K0W59_003215"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HBI3024148.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12102..13109
                     /locus_tag="K0W59_003216"
     CDS             12102..13109
                     /locus_tag="K0W59_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HBI3024149.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13165..14466)
                     /locus_tag="K0W59_003217"
     CDS             complement(13165..14466)
                     /locus_tag="K0W59_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBI3024150.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKHRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTLPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3807 a         3477 c         3732 g         3511 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgtagg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct acttagaaga aatgttcagg ctggatggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gcccggactc ggaatcgaac caaggacacg
     1261 gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc
     1321 tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc
     1381 atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag
     1441 cggtattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca
     1501 gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat
     1561 cgacggtgtg gtcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat
     1621 cagtaatggg atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc
     1681 cgtaaagaaa tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc
     1741 gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata
     1801 attctgaata aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc
     1861 tttggcgact gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc
     1921 aagtagcgta gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc
     1981 aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa
     2041 gcgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa
     2101 agggccgatg accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact
     2161 ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag
     2221 gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct
     2281 ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc
     2341 tcatgcggtg aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg
     2401 cttttactga tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca
     2461 gggttttcca ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc
     2521 aatgaatatt gccgccagta gtgaactggt atcccgactt tcttctcatc gccgcgtggt
     2581 ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag
     2641 tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta
     2701 ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca
     2761 gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt
     2821 ttatgacacg ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca
     2881 tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg
     2941 tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat
     3001 tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa
     3061 aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt
     3121 aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc
     3181 gctgattcag gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt
     3241 cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga
     3301 cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc ctggcgatta ttcagctggg
     3361 aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctctg
     3421 tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc
     3481 ggatatctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca
     3541 gttggtgcac aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa
     3601 ccatatccgc ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct
     3661 ccatgcttct accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat
     3721 tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc
     3781 gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga
     3841 tggcaaattg tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag
     3901 ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt
     3961 tgatccgtgc aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag
     4021 cgactttggc gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc
     4081 ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa
     4141 gctggcacaa ctggtggcga tgctggcgca atttgaacag catattgagg atgactcgtc
     4201 gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac
     4261 cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct
     4321 acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc
     4381 gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg
     4441 aattgcggca gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga
     4501 agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt
     4561 gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa
     4621 acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc
     4681 gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt
     4741 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca
     4801 accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc
     4861 tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac gtcccttgtg
     4921 cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     4981 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     5041 gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca
     5101 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     5161 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg
     5221 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     5281 gcagttccgc gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa
     5341 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     5401 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     5461 caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca
     5521 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     5581 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     5641 caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca
     5701 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     5761 atttgtcggc cacaatcccc agtagcgcag gcataaatac cgcggcgata cccagtgagc
     5821 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     5881 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     5941 gcttaagatt catgttaatt tcctcacatc gtgattcgga tgagtgtttg aaaaagcaca
     6001 tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt
     6061 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     6121 tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg
     6181 cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg
     6241 tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat
     6301 tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac
     6361 gccgcgatgt tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg
     6421 cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg
     6481 atttcccctg cgagcggaac acatctttcc cggcagtatt ttgtaccacc tgcattaatc
     6541 ccagcgcatc ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg
     6601 ccagaatcag cgactcatca acgccatatt tccgtgacgc ctggcggacc atgccgagat
     6661 atttgtgcgc acgtttatca aggtggttcg gcaccatgtt aatggtgacg ctgtagatga
     6721 tacgtagccc gttgctgcgg cttttcagac ggtttttcag cagataatcc gcgaagttgc
     6781 ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga
     6841 aaggttcttt cgaaattgta atatcatcaa cgtcggaata gagatcgacc gaactcggat
     6901 cgtcacccat cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc
     6961 cggcgatggt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat
     7021 attgatcggt gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc
     7081 agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt
     7141 tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg
     7201 gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt
     7261 tttatgcaag ctgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta
     7321 tagccctcga tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc
     7381 agcttgcggt gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt
     7441 ttgtgctgcc actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc
     7501 tcgccggggt acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa
     7561 atcgttctgc tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg
     7621 cgtaactgct gtaacaaacg ctccacggga gccgccaggc caactgacgg cggttgcgct
     7681 aagttatacc agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc
     7741 cacataggca caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc ggtcagttgc
     7801 gtcaggttat cggcagcaat ctgccgttgt gccagccaat gccgcaaact ttcttcgtcg
     7861 gcaaactgcg ggaaacagta taaaccgccc cacaatccgc tcggcggacg ctgcgccagc
     7921 aatacttcat cttcgtgctg taacaggaga aagtagccgg tgcgctccgg cagcgtctgt
     7981 ttcggttttt tgcccggata aagcgaccag ctattgttgg tggcggcaat acatccgttt
     8041 tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc aaatcatcgc gcccaaatcc
     8101 atcatcgcct gattaaaccg ttccacgccg accgcgggcg tcacctgctc gctcaaactc
     8161 catagtttat tctcgacctc ttttttccca ggccagccgc ttacagcata gcagcgcgcc
     8221 agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct tgcccagaga aagcgagaga
     8281 atcgcgcctg cggtggaacg cccgacgccc ggtaacggcg cgacttcttc aaaggtttcc
     8341 gggaatttac caccgtgtaa ggtcgccact tgttgtgccg ctttatgcag gttgcgcgcg
     8401 cgggcgtaat agccaagccc ggtccacaag tggagaactt cgtcgagcgg cgcattggcg
     8461 agatcagtca ccgtcgggaa gtgcgccata aagcgttcaa aataggggat aacggtcgca
     8521 acctgagttt gttgcaacat cacttctgag agccatactt tgtagggcgt cttgtcaatt
     8581 tgccagggca gcgttttccg cccgtattta tcgtaccagt ccagaacctg ggctgaaaat
     8641 tgcgacgctt gcatggtcac cgacttcact gttgttgggg gcaagattgc agcacagcga
     8701 cggcagggtg taaaccggaa ctttccgcag ccacggcctt atcatgcttg catccggcaa
     8761 ctaactttgg ataatgcccg ttttcagaac actttcacaa gcgactaaat ctttatgaaa
     8821 aacgacgtca tttcaccgga atttgatgaa aacggccgcc cactgcgccg tatccgtagt
     8881 tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac atgcgctgga aaattactgg
     8941 ccggtgatgg gcgttgagtt cagtgaagat atgctggatt tccccgcgct ttttggccgt
     9001 gaagcgccgg tgacgcttga gattggtttt ggcatggggg cgtcgctggt ggcaatggct
     9061 aaagatcgcc ccgagcagga cttcctcggc attgaagtgc attcaccggg cgttggtgcg
     9121 tgcctggctt ctgcgcatga agaaggttta agcaacctgc gcgtgatgtg tcacgatgcg
     9181 gttgaagtgc tgcataaaat gattcctgac aattcattgc gcatggtgca gctctttttc
     9241 cctgacccgt ggcacaaagc gcgccataat aaacgccgta tcgttcaggt gccgtttgcc
     9301 gaactggtaa aaagcaaact gcagctgggg ggcgtattcc atatggcgac cgactgggaa
     9361 ccttatgcgg aacatatgct tgaagtgatg tcttctattg acggttataa aaacctgtca
     9421 gagagcaatg attacgtacc gcgtccggca tcacgtccgg tgacgaaatt tgaacaacgt
     9481 ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg agagggtgaa ataatggcaa
     9541 agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag gaattaggat
     9601 tttcggtggc atggcgattc ccggaaggta catcggaaga acagattgat aaaatcgttg
     9661 atgactttat taacgaggtt atcgaaccga acaaactggc ctttgacggc agcggttatc
     9721 tggcctggga aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa gaacatcagg
     9781 cgattgtgcg taagtggctg gaagagtgca aactggatga ggtacgcacc agcgaacttt
     9841 tcgacgtttg gtgggactaa gaaagcatac gggcgatgac aaatgcaaaa ctgcctgatg
     9901 cgctacgctt atcaggccta cagagatgca cgatcgagta ggcgggataa ggcgtttacg
     9961 ccgcatccgg catggaaaac gcgtactttg ttatcaatct ggggccagca tatgctggcc
    10021 tgatttgttc ttgagggaag actatgatgc gcaaaatgct gctggcggca gcactttcag
    10081 tgacggcaat gaccgctcac gccgactacc agtgcagcgt cacgccgcgt gacgatgtga
    10141 ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg atcacgccag
    10201 acggcaacgt gatgtataac ggtaagcaat attccctgaa tgccgcccag cgcgagcagg
    10261 cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg gattgatgaa ggcgcgaaaa
    10321 gccgcgtcga gaaagcccgt attgcgctgg ataaaattat cgttcaggag atgggcgaca
    10381 gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca gcttaaagag cagatgaatc
    10441 gcattatcga aacgcgcagc gatggcctga cgttccacta taaagccatt gatcaggttc
    10501 gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg aattttacag gacagcatta
    10561 atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa cccattacag aatgtgctgg
    10621 gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg gaaaaagcag gaaaaagatt
    10681 tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac tctggaagat agccgcaaag
    10741 ccctggtcgg gaatttaaaa taatcctcta ttttaagacg gcataatact tttttatgcc
    10801 gtttaattct tcgttttgtt acctgcctct aactttgtag atctccaaaa tatattcacg
    10861 ttgtaaattg tttaacgtca aatttcccat acaaagctaa gggataatgc gtagcgttca
    10921 cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc actggttatg
    10981 ggttttagtg gtgcagcatt ggcattaccc aatatcacca ttttagcaac cggcgggacc
    11041 attgccggtg gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa agttggcgta
    11101 gaaaatctgg ttaatgcggt gccgcaacta aaagacattg cgaacgttaa aggcgagcag
    11161 gtagtgaata tcggctccca ggacatgaac gataatgtct ggctgacact ggcgaaaaaa
    11221 attaacgccg actgcgataa aaccgacggc ttcgtcatta cccacggtac cgacacgatg
    11281 gaagaaactg cttacttcct cgacctgacg gtgaaatgcg acaaaccggt ggtgatggtc
    11341 ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc cattcaacct gtataacgcg
    11401 gtagtgaccg cagctgataa agcctccgct aatcgtggcg tgctggtggt gatgaacgac
    11461 accgtactgg acggtcgcga tgtcaccaaa accaacacca ccgacgtagc gaccttcaag
    11521 tctgttaact acggtcctct gggatacatt cacaacggta agattgacta ccaacgtacc
    11581 ccggcacgta agcacaccag cgatacgcca ttcgatgtct ctaagctgaa tgagctgccg
    11641 aaagtcggca tcgtttataa ctacgctaac gcatccgatc ttccggctaa agcactggta
    11701 gatgcgggct atgatggcat cgttagcgct ggtgtgggta atggtaacct gtataaatcc
    11761 gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg cagtagtgcg ttcttcccgc
    11821 gtaccgacgg gtgctaccac tcaggatgct gaagtggatg atgcgaaata cggcttcgtc
    11881 gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc tgcagctggc tctgacgcaa
    11941 accaaagatc cgcagcagat ccagcagatt ttcaatcagt actaatcacc tcgtcccggc
    12001 atcgtgccgg ggctttttca cttcagactt acgtccattg ccaattttca ttatcctaat
    12061 gataatcacc ggaataaatt attccgcgcg agggttttcg ggtgaaaaag caatggattg
    12121 ttggcacggc gctgcttatg ttgatgactg gtaatgcctg ggcagatggc gaaccgccaa
    12181 ctgaaaatat cttaaaagat caattcaaaa agcagtatca cggcattctc aagcttgatg
    12241 ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc cacctggtca gcggaaggcg
    12301 atgtctcttc cagtgacgat ctctatacct gggtcggtca gttggcagat tacgaactgc
    12361 tcgaacagac ctggacgaaa gataaaccgg taaaattctc ggcgatgtta accagtaaag
    12421 gaacgcccgc gtctggctgg tcggtgaact tttactcttt tcaggcggca gccagcgatc
    12481 gtgggcgggt ggtcgacgat atcaaaacga ataataaata tctgatcgtg aatagcgaag
    12541 atttcaatta tcgctttagt cagcttgaat ctgcgttgaa tacccagaag aactcgattc
    12601 ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg cagaaagcag
    12661 cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa gaagccttta
    12721 aaaaaattca ccaacagcgt gatgagttta ataaacagaa cgatagcgag gcgttcgcgg
    12781 ttaaatatga caaagaggtt tatcaaccgg cgattgcggc atgtcataaa cagagtgaag
    12841 agtgttatga agtgccgatt cagcagaaac gagatttcga tatcaacgaa caacggcgac
    12901 agacttttct gcaatcacaa aaactcagcc gtaaattgca ggatgactgg gtaacgctcg
    12961 aaaaagggca atatccgtta acgatgaaag tttcagaaat aaacagtaaa aaagtcgcga
    13021 ttttgatgaa aattgacgat atcaaccaag ctaatgaacg ctggaaaaaa gataccgaac
    13081 aactgcggcg caatggggtg attaagtaac tcatgttaag tgcctttgcc ggataatata
    13141 aaaagttatc cggcaaatta ttttttacat tagccctagt agctgtggaa tgaacaagga
    13201 tatttgcgga atgtaagtga tcatcatcaa cgctactagc aatgcagcat aaaatggcag
    13261 caatggtttg attaaatgtt gaatcttcac acccgagata gaacaaccaa caaatagcgc
    13321 actacccacc ggcagtgtta acagcccaat acaaaggttg gccaccatca taataccgaa
    13381 atgtactggg tccattccca gctcttgtgc aatcggcaag aagatcggtg taaagattag
    13441 tacagccggt gtcatgtcca taaagatgcc gacaattaat aagacgatat taatcagcag
    13501 caatatgatc agtgggttat cagaaatccc catcaatgcg tcgctgatca tataaggaat
    13561 atcagcatta gtcattgccc aggacatgcc cacggaaaaa ccgatcaaca gtaatacaat
    13621 cgaagtggta acaaccgact ccagaatcag tttgggtaaa tggcgccatt ttacttcccg
    13681 gtaaatcagc accgagagaa taaatgtgta gacaacggca attgctgacg cttcagttgc
    13741 ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac accagaagca aacttggtaa
    13801 ggcatccaga aacgctttac aggcctgagc aaaggttgct ctttctgaaa gcgggtagcc
    13861 acggtgttta gcaataatgc cgcagactat cattaccgca agacccatta agatccctgg
    13921 caaatatccc gccataaaca gcgatgctac agaaacgccg ccagctgtca gggaaaacac
    13981 aattaacacg tttgagggcg gtatcagtaa tccagtaata caagatgaga cgtttacagc
    14041 ggtagaaaat gccggatcat aaccttcttt ggtctgaatg ggattcagtg tgccaccaac
    14101 ggcagcagct gccgcaacgg ctgaaccaga aatggaacca aacatcatat tcgccaggac
    14161 gttcacatga ccaagcgaac caggaacgcg gccaaccatt acctgggcga ggttaatcaa
    14221 acgtatcgca ataccgccgc tgttcatcag tgttccggca aagatgaaaa aggggattgc
    14281 caacagggca aagttatcca gtccattagc cagtcgttgc gaaacagtga ttgccgcgat
    14341 atcccaggga aacatcaaaa gcattgaagc tacagtggca ataccgattg agaacgaaat
    14401 gggcacacca ataaagacca gcacaaaaaa acagccaaac agtgtaatcg cgatataact
    14461 atccattaac tggcctcctg cgcaggttgt ttcagttgtt gaatgctttg attaataaaa
    14521 cacaagg
//
LOCUS       DAETPZ010000092        14162 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.092, whole genome shotgun sequence.
ACCESSION   DAETPZ010000092 DAETPZ010000000
VERSION     DAETPZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.092"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..915)
                     /gene="wcaD"
                     /locus_tag="K0W59_003218"
     CDS             complement(<1..915)
                     /gene="wcaD"
                     /locus_tag="K0W59_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HBI3024151.1"
                     /translation="MSTSIRICSYLLLLLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSL"
     gene            complement(890..2107)
                     /gene="wcaC"
                     /locus_tag="K0W59_003219"
     CDS             complement(890..2107)
                     /gene="wcaC"
                     /locus_tag="K0W59_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HBI3024152.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKHQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2104..2592)
                     /gene="wcaB"
                     /locus_tag="K0W59_003220"
     CDS             complement(2104..2592)
                     /gene="wcaB"
                     /locus_tag="K0W59_003220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HBI3024153.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2600..3433)
                     /gene="wcaA"
                     /locus_tag="K0W59_003221"
                     /pseudo
     CDS             complement(2600..3433)
                     /gene="wcaA"
                     /locus_tag="K0W59_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3526..5688)
                     /gene="wzc"
                     /locus_tag="K0W59_003222"
     CDS             complement(3526..5688)
                     /gene="wzc"
                     /locus_tag="K0W59_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HBI3024154.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPSVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5691..6134)
                     /gene="wzb"
                     /locus_tag="K0W59_003223"
     CDS             complement(5691..6134)
                     /gene="wzb"
                     /locus_tag="K0W59_003223"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HBI3024155.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6140..7279)
                     /gene="wza"
                     /locus_tag="K0W59_003224"
     CDS             complement(6140..7279)
                     /gene="wza"
                     /locus_tag="K0W59_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HBI3024156.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLIPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7588..7737
                     /locus_tag="K0W59_003225"
     CDS             7588..7737
                     /locus_tag="K0W59_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024157.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            7938..9521
                     /locus_tag="K0W59_003226"
     CDS             7938..9521
                     /locus_tag="K0W59_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBI3024158.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9973..11826)
                     /gene="asmA"
                     /locus_tag="K0W59_003227"
     CDS             complement(9973..11826)
                     /gene="asmA"
                     /locus_tag="K0W59_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HBI3024159.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11848..12429)
                     /gene="dcd"
                     /locus_tag="K0W59_003228"
     CDS             complement(11848..12429)
                     /gene="dcd"
                     /locus_tag="K0W59_003228"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HBI3024160.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12521..13162)
                     /gene="udk"
                     /locus_tag="K0W59_003229"
     CDS             complement(12521..13162)
                     /gene="udk"
                     /locus_tag="K0W59_003229"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBI3024161.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVKVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13480..13899
                     /locus_tag="K0W59_003230"
                     /pseudo
     CDS             13480..13899
                     /locus_tag="K0W59_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            complement(13924..>14162)
                     /locus_tag="K0W59_003231"
     CDS             complement(13924..>14162)
                     /locus_tag="K0W59_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBI3024162.1"
                     /translation="GSSIRGEHPSRRGNKALKRALFLSAFAALRDPLSRAYYTRKMSQ
                     GKRHNQVLIALARRRCDVLFAMMRDGTFYTPQGS"
BASE COUNT         3281 a         3739 c         3593 g         3549 t
ORIGIN      
        1 aagtgagcca aatctgacta caccgtcaat atgggtcaaa gaataaccga ccatcaccag
       61 cggaccgaca atacgataat acgatgacga tccttccgta cctaaatcgc ccagacgggt
      121 ggaaatataa ggaaacgcga ttaccacacc aaccaggaat acagccagag aaatcaatgc
      181 taacggtaat tttttcttaa tcgcctcttt attcagatat tgaaatgccc actccagcaa
      241 ataaaacagg ataaaggtca taacccctga aaacgatccg gataatatta tccctgcgag
      301 aatcatagca tctgttttag gcgttttgat accaaactgt ttgatgctga gccaaattga
      361 gattaatgcc agagcgaaaa atgccggttc gaaataaagt gctgtcgtgc gcttgccgcc
      421 gaatttaatg aaattcagca catagctgtt gctgtaaatc agatatttcg aaatcgactc
      481 cataatactg ctgccaccgg tgaggataat ttgcgccatc tccaccgccg ccagcgccac
      541 caccagtccg accaccagat aaaagaaacg taatatctta cgatggttgt gtggcgaaat
      601 tgttttaaaa cgaatactcc acaccatgcc aataatgatc acaatataga caaacagcat
      661 agttgaagtg acgtatttac cggcatccaa cgactgacca aacaggtagt taaacgccgt
      721 gagtcccgcg ccaatcccta aggcaatcat caatttttta atgctgatgc gttctaaaaa
      781 cagcagtaac aagacaggta aaaaagtgac gatggtgatg gggaaacttt cgccaagctg
      841 ggcgattttg acgttaacca gcaggtagat cagtagcagc agcaggtagc tacagattct
      901 gatagaagtt gacatactcc tccagcatct gttgtccact gtaggcggcg cgactgcgtt
      961 ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc gatttccggt ttgcttaact
     1021 gtaccagttg cagcacctct tcttcactga cggttttacc accggatttt tgcagcactt
     1081 cccgcgccgc atcgctatgg gtggcaatca ccggcacgcc aatcgatagc gcctcacaca
     1141 aaatcagcgg gtagttatcg acacgagaac tgaacaccag cgcatccatc tgattgagcg
     1201 cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt aaccacgttg ccagcggtga
     1261 acggcgagaa cttaccaaag gtatgcagtt cgattttgtc gcccagcgcc atcatctcac
     1321 gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc atgcgccacc accgcgattt
     1381 tcggcttgcc ctgagtttcg cgcaccggag gcagatctgc cagaatcgct tcggttgcca
     1441 tatcaatgcc attattgata atccggcaac gccctggccc gtacagacta ttgaaagcgt
     1501 cagccacatg ctggctgggg gaaataaact gacagcccag cgccagcatc tcgcggaata
     1561 actggtgttt gcccgccacc agctggtgcg cgcgatcaat cttcaccggc ggatagttat
     1621 ttaaggtcgg gcatttctgg cagcccgttt tccagccttc gcaaccgtcg gtaaaggcgc
     1681 agcgtccggt tacactccag tggtcgtgca gcgtccagac cagggtgaca tccggtttat
     1741 ggtttttcac tttttcgcaa aagcgcacca cgctgttaag attcaaccag tagctgtgca
     1801 gcacatgaaa atgcaggacc accggacccg gtgtgcgagt aatggtgcga tataactcat
     1861 tgaaattgcc gaacagatcg cgattaaaca gacgaaacaa cgcaatgttc gccatcgcgg
     1921 tcatccgcgg cgtatgtttg atgacctgcg gatagttctg atggctgaca ctctctttgc
     1981 cgcctttgcc gtagccgtag acaaaatgtg acgccagccc ctgttgcagc gcacgctggt
     2041 ggagatctaa cgccacacct gctgccccgc cttccgccag tcgcacatta aattgcagaa
     2101 tattcattta attaccttca ctcgcgcttt ttctcccacc accagcgcgt tgtccgggac
     2161 agagtcgagc accacgctgc ccgcacccac ggtgacgttg ttaccaagcg tgatatcacc
     2221 aagaataatg acgttggcac cgagttcgac gccgttgcca atgtgtggac atgccatgtt
     2281 atcggcaccc cgattgccga tagtgacgcc gtggcgaatg gtgaaatcat cccccgccac
     2341 cacgttttta ttgatcacta cggcgtaacc gtgatggata gtaaagcggc ggccaatggt
     2401 cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg ataatgcgat acagcaccag
     2461 cagcggggcc gcccacagat tgttgaggac gttctttttg cgccacaccg aacaaaaatg
     2521 agcgacgcga taggcaagta ccatgcagca cgggcgtaaa ctccagctgt tggcgcgcag
     2581 atcttccagc atctttagcg ccccgaatac catcagccag acgtttgccg ttacgcaccg
     2641 acagcagcgt caacaaggtg cgccaggtca tacgtttatt gcggatctgg tagggggtaa
     2701 acagctgata ttttttactg gcgcggtcga atttgtcttt gtgcttgcgg taaaagtgga
     2761 agtatccgga gaattttttc ggtgacgagg tgatctgcat ttcgccgtga ttgatatgca
     2821 ggatctgcgt cgcctcttcc actttccacg gttcgccgta ctccaccacc atccgcagga
     2881 atatgtcgta atcctgtgct gctttcagtt cggtatcgaa caaacactct ttgaaacgcc
     2941 atgcccaggt aaagacctga ttgccaatga tattacgttt atagaacagg cggcgtgagt
     3001 acggcgattt gggatacagt ggtaagctcg ccggttggga ataaacttct ccctggcaaa
     3061 cgtagtcgtt agcgtacaaa aaggcgtgtg tgaccagttg ctgtttatgg gcgaggaaga
     3121 cgctcaggcg gttgggcgtc cattcatcat catcatcgat cccggtgatg tattcccctt
     3181 gcgccagcat aatcgcctgg ttacgcaccg cgcacgcccc gctgttaatg tcgttgtgga
     3241 tgtaagtgat acgcggatcg ttgagggcag tgacgtactg ttgcagttgt tcccaggagg
     3301 tggagcaatc atccacgatg atcatctccc agttgctgta gtcctggcgc aggaccgatt
     3361 ttatcgcccg aatcgccagt tgttggcggt tccaggttgg catatagatt gagatcagcg
     3421 gattgtcttt cataggttgt tctcccggct tatgagctgg tttgcttgcc ggatgcggcg
     3481 tgagcgcctt atccggccta cggggcggtg cgaatgcagg cgtcgttatt tcgcatccga
     3541 cttatattcg tattcgtaat agccataatc ctgatacgcg ctggcgcggc ggaagatgga
     3601 gttcagaatc acccctttca ccggaatacc gttttgctca aagcggctca gactggtttc
     3661 cacttctttc aatgtgttga ccgcataacg cgccaccatt aacgtggttc cgacatgacg
     3721 accaacaatt gccgcatcgg tcactgccag aatcggcggc gtatcaatca acaccaggtc
     3781 atagttttta ctcgcccagt tcaccagttc ggcaaagcgt tcgctcatta acagttcaga
     3841 aggatttggc ggcacctgac cgcgcgggat caggtcaaat ttggcaatag aggtcggttt
     3901 agcagcggta gtaatatcgc cctgaccaat cagaatctcc gacagcccat tgacattatt
     3961 ggtgcccaac agctcgtggg tgtagccttt gcgcatatcg cagtcgatca acaacacgcg
     4021 tttattggtc tggctgatca ccgccgccag gttggcgcag acaaaggttt taccgattga
     4081 cgggctaacc ccggtcatca tcaacacatt gttctgcgcc tgcatcatcg cgaagtgcag
     4141 actggtacgc agactacgga tggcttcaat cgccagatcg gttggattcc ccactgccag
     4201 tagctggctc tgtttatagc gtttaacccc tttgatggtt ttgacgctat cgcgcgcttt
     4261 ctgccactcc gacagcggga tgctggcata gacgctgatt ccgtgttctt ccagcacttg
     4321 cgggctttcg atgccgcgat taaacaacga gcgcagcagc acacccacga tagagagcat
     4381 caggccaagg ataatcgcgc cgaggataat cagccctttc ttcggtttta gcacgctagg
     4441 ctgggtgatt gccgggtcaa taatgcgcac atcgccgacg gtgctggctt cggtgatttt
     4501 cagctcctgc tctttattca gcagttgcat atagacctgc tgaccagact cgacatcgcg
     4561 ggtcagacgg acaatttcct gctgggtttt cggcatcgcc gttacgcgac cgttaagttt
     4621 ggctttctct tcttccagcg cctgacgttt ctccagcagc gtgcggtacg ccgggtgaac
     4681 tttggtgtac agcttggaga tttccgcctc tttaaaggtc agttcgttca actgcgcgtc
     4741 gatgttcacc atcgaatcga gcaccgcttt cgcttcaagc ggcagatcaa cagaatcttt
     4801 atcctgacgg aaggcattca gtttgttttc ggcaacatcg aggcggctac gtacttccgg
     4861 taactgttgc gcgaggaaag cgaggctttt cgacgcttcc gccgatttgc gctcaatatt
     4921 ttgttcctga tagttacgag cgatgctgtt aagaatgtcg cggatctgtt cgcgatcttc
     4981 accggtataa gtcaggctca gtacgcctgc gtctttgccg ttctccgtta ccgtcaggct
     5041 gttttgcagt tggttgatca tccccaacgt ggagtatttg gtgacggtaa actcactgcc
     5101 cgggcgggcg tgaatggctt caaccatcag cgtgacgcct tcttttttca gcatctggcc
     5161 cgcttgccca cgggcgctaa agccgccatc actgctcagg gtgtagtttt tgtcatccag
     5221 cacattaagc gtaaacacct gatccgccat ctctttcggg cggttaaagg tagtcacttt
     5281 caccgtctcg ttctgacgcc ccatcaggcg atcccagccc gccccgaaaa tcgggaacgt
     5341 gtttttgctc accgcaatat cgaggtcgag atcgtccacc gttttaccaa gcaccagacg
     5401 cgaacgaatc aactggatct cggcgtccga tgcaggcggt ttgttggcta atgccgagcc
     5461 gatatcctgc accagcgaat tgccgctgtt ttgctcgatt tgtaccagtg cgtcggcact
     5521 ataaatcggc gtggcgaaga aagtgtaaac cacggcacag agggcaaata cgacggtgat
     5581 gccaatcacc caccagcgcg cttcaatgac ggtgccgacc aggcgaccaa tatcgatttc
     5641 atcactgccc gttaccggag cggcatgttg ttttactttt tctgtcattc ttatacctgc
     5701 tctgcgttca atgcctgcgc ccactggcgg gcagaccgtt caagtaatgt gtacaccgct
     5761 gcaaacgttt cccggctttt gcgatacgga tcggggattt cacattcgtt atcccagtga
     5821 ccaaacagca tcactttgcc gcgcatctcc ggtgccatct cgcataagcg ttcgatatgg
     5881 cgcttatcca tggtcaaaat caggtcatag ttgcgacaca ggcggcggct gatttgtcgt
     5941 gcacagtggc cttccagaga cagttgatgt tctgcggcga cgctgatagc ggtaggatca
     6001 gcgcccttac cgaccagcgc gccgagtcca gcggactcca ctttcagttc cgggtgataa
     6061 cgttgcagta agcgttccgc catcggggaa cggcaaatat tgcctacaca gacaactaag
     6121 atgttgttaa acatgacgat taccagttat gaatgtcgct ggctgtatcc gtcatgtaac
     6181 ggacaccgct aatagttggc agcaactgat tgatcagacg gttccagcgg gaaaccgggg
     6241 cggtggtgac atacaccacg tcataaggtt gcaggcggaa ttctgtcgcc atcaccagcg
     6301 acgtcgcatc ggacatatcc agctggtaga tattggcaat cttgccgtta cgcccgccct
     6361 cgcctttcag cggacgaatg acaaagatgc cgctggcgtt ggaggtggtc atgtcgatac
     6421 cttcggcatt gcccagagct tcagtcaggg tcatgccgct aaagtccatt ttcagggtgc
     6481 tctgtttctt cacttcaccc atcacaaata ctttcagatc atcattacgc ggcacgtaga
     6541 gaatatcgcc gggataaagc agtcggttct gattgaggtc gccgttttgc atcagcgcct
     6601 gtaaagaaat gcgttcttca cgaccattgt gtgttagcac cacgttgcgc cagtcagcgg
     6661 tgtcggtcag gccacctgcg gcgttgatgg cgtcgagaat ggtcagtggt acgttggtga
     6721 tcgcctgttg accggattta ttcacctgac ctgagatata ggccttttgt gagcggaagg
     6781 cggcgatatt aacgtccacc tgcgggtcag cgatgtatgt cgctaagcgc ccggtaatat
     6841 cactgcggat ttcagcgagc gttttcccga ctacgtgaac cttgccgata tacgggtaaa
     6901 acatagtgcc gtcaggctgt acccagttgc cggtgtcgct ggagctgcgg tactgaccag
     6961 ctggcgtggt gagttccggg tgatcccaga cggtgacatt aagaacgtcc cccggcccga
     7021 cgcgatactg gtaattcgcg atctcgcttt ccagcgtcat attggggcgc gctacattcg
     7081 ggcgtgggcg taattggtca atcaggcgcg ggatcagcgg ataaacattc accattttgt
     7141 cgagatcgaa atcagcgtcc tgctgtttga tgacgtcttt gcccatcgtc gacatattgc
     7201 tgcccggaag tactgtgcaa ccgcttatca aggttactga caccaataat ggcatcaatt
     7261 tcattttgga tttcatcatt gtttatttat cactttggca gagtaattat cctgtgcact
     7321 attaatagca atgtcgccat gcacatttac cttgcagtta attgaataaa aatttaactg
     7381 gcatcagtcc taaaaaaatt gatttcatca gcaggctatt gacagaataa ttcagactgg
     7441 tctttcaggc atccagacac gctaccgccc ctggcttttt agctaccaat acactgattg
     7501 agcttaattt ttcaaaccct ctctgcatac aatcgttgat gagaaagggt tattgcggaa
     7561 attaacttcc gaatataagg tgacattatg gtaattgaat attggctcgc caataatgca
     7621 ggaggaagtg ttacagctaa cggaatagca ggcaaggtaa caattcggca attggctatt
     7681 tttaagaatt atattaagtg tcattcaata tggtttttag gagtttcttt aggttgacaa
     7741 tatttaatat tgcgtctcca catgcgatat ttcttaaata atgttttatt attaccgctt
     7801 ttaattcagg gataatgtga ggttattatc ctcaaataat atgagataaa tagtgctgca
     7861 acattgcatt tttgccccga tttatccatg atcgaattgt gacatttgtc atacaacgaa
     7921 taggttttgt acttactatg gaatggattg ccgatccgtc tatctgggcc gggttaatca
     7981 cgctgattgt gatcgaactg gtcctcggca ttgataacct ggtctttatt gccatcctcg
     8041 ccgaaaaact accgccgaag cagcgcgacc gcgcacgggt taccgggctg ctgctggcga
     8101 tgttaatgcg cctgttactg ctagcgtcaa tctcctggct ggtcaccctg actcaaccgc
     8161 tgttcagctt ccgctcgttt acctttagcg cccgcgactt aatcatgctg tttggtggtt
     8221 tcttcctgtt gttcaaagcc acgatggagc tgaacgaacg gctggaaggg aaagacagca
     8281 ataatcccac acaacgcaaa ggtgcgaagt tctggggcgt ggtgacgcaa attgtggtac
     8341 tggacgccat tttctcactc gactcagtga ttaccgccgt cgggatggtc gaccatttac
     8401 tggtcatgat ggccgccgtg gttatcgcta tcagcctgat gttgatggcc agcaagccgt
     8461 taacgcaatt tgttaacagt cacccgacca tcgttattct ctgcttaagc ttcctgttga
     8521 tgattggctt tagcctggtg gcagaaggtt tcggcttcgt cattccgaaa ggctacctgt
     8581 acgctgccat tggtttctcg gtgatgatcg aggcactcaa tcagttggct atctttaacc
     8641 ggcgacgttt tctttccgct aatcagacgc tacgccagcg gactaccgaa gcggtaatgc
     8701 gcctgcttag cgggcaaaaa gaagatgcgg aactggacgc cgaaaccgcg tccatgctgg
     8761 tggatcatgg aaaccagcag atctttaatc cgcaggaacg gcggatgatt gagcgggtac
     8821 ttaatcttaa ccagcgtacc gtcagcagca ttatgacgtc gcgccacgat attgagcata
     8881 tcgatctcaa cgcgccggaa gaagaaatcc gccagttgct ggagcgaaat cagcatacgc
     8941 ggctggtggt taccgacggt gatgacgcag aagatttgct cggtgttgtt cacgttatcg
     9001 acctgctaca acagtcactg cgcggcgaac cgctcaacct gcgggtgttg attcgccagc
     9061 cgctggtgtt cccggaaacc ttgccgttgt taccagccct ggagcagttc cgtaatgccc
     9121 gcacgcactt tgcttttgtg gtggatgagt ttggctcggt ggaagggatt gtgacattaa
     9181 gtgacgtcac tgaaaccatt gccggtaact taccgaacga agtggaagag atcgacgccc
     9241 gccatgatat tcagaagaat gcggacggtt cctggacggc gaatggtcat atgccgctgg
     9301 aggatctggt gcaatatgtg ccgctgccgc tggatgaaaa acgtgaatat cacaccattg
     9361 ccgggctgtt gatggagtat ttgcagcgta ttccaaaacc tggcgaagaa gttcaggtgg
     9421 gggattattt gcttaaaacg ttgcaggtgg aaagccatcg cgtgcagaag gtgcagatta
     9481 taccgctgcg taaggatggc gagatggagt acgaggtgtg atggtatcgg ccattttacc
     9541 caatgctgcg taacgcctta taacttcagc aatgaagcgt tgtctgtggt aaacggacat
     9601 cacagcgggg gtgtgggtcc ggctgtaaat cgccgaatcg tttcctgtag gctggggcag
     9661 cccacatgga tgtgggctga gggcgcgttt tacagggatg ttacctcgcg cccgtacccg
     9721 atagccgaaa ggaataagat gagggaaccg cgccagcggc gatttaccgc cgggagcccg
     9781 ggttgccagg gtggtggcga ttgagccacc ctggcacgtt caccggcttg agcgttccat
     9841 agtagcgagg aacatcaagt gaacggaaca gccttcgatc ttacatattc agaatataat
     9901 agtgccgccg cgaacgcctg atgcgacgct ttacgcgtct tatcaggcct acaggacagg
     9961 tcacctggta acctacatct tctccagcaa cttcttcaca tctttgccat tgcgggaatc
    10021 tttattccgc tcggcccagt cattcaagcg acgtttcgct tcgtcctgta gatgtttgcg
    10081 cagtaactga tccacttgca ggctgtaatt gagttgctgc cagttgccat aaacccgcag
    10141 cggtactggc gtttctttga ggaaatcaat cagtttgctt tccccgttcc agccacccac
    10201 gacccgaata tcaaactggg tgtcgcaggt ttgatctgcc agattcaaca tgccttcccc
    10261 tgtcagcgcc agcacaggcg attgaccttg catgtcgttt aacgtcacga cgccatcctt
    10321 caacgtcaaa tcggtggtaa agcggtcaag ccgcgtcacg ttatcgaagt tttcagcggc
    10381 cttcacatca ccgccattac gctctaccgc ttgctgaatc atctgctgga agttcatccc
    10441 ttccatacgt gtgtcggtca tctcaacatg cgcctgccct tgccagttgt ggcggaatgc
    10501 gtcggcatct atgtcagcac cggagaaatc accagccagt gacatttttc cggtcaacga
    10561 aatcggatag ttaaacgcct tcagaatggt accaatctca acgttctcca gccgtggctg
    10621 gaagtttatc cgcggattta ttgatgtcgc gtccagcgtg cccggcagtg aaacctttcc
    10681 accgttaagc ttgccctgca gttgagtaat ttccagcaaa cccgacttgt tggtcatttg
    10741 cgtggcaaca tctgtaaaat tcattccgcg ccagcgcacg ttactggcct gcaacaaaat
    10801 atcagctgta aagccttgca gtccctgata ggccggttca tcaatacgcg aagaaatgac
    10861 cgggcgcggc aacgttgatt ggctctgccc ctgctgcgcg gcaccgtttt caccattcgc
    10921 ggtttcatta agcgggatga ggttgtcaag attcagttgc gggaattgca gtctcagctg
    10981 ccattccggt ttctctgtca gcgtgacctg tgcttgcccg ctcagcgtac tgtcattggc
    11041 ggtcaaacta atttggttaa atgaaaggcg tttatgtgac tcctgccact gggactggaa
    11101 actcccctgc ccctgaatac cttgttttgg taaatcggca ccctgcaact gccagttaat
    11161 ttgttcaata gccgctgtta aatcatgcgg ataatcagaa gcatctaccg taccgttaag
    11221 ggatatcgtg agatcgcgct gatcgcgatt aacccgcccg gagaactcaa atgagccacg
    11281 atgttgggga tcttgttcca tttgcaggcg gatattgcgg attgtcacct gctcgtcatc
    11341 ttcatgctgg aacaccagaa cgctgtccgc caccttaaga ctggatatat caaacgacca
    11401 tccgcgatcg tctgacagat ccggcaaggt attgtcgcgc ggtgcaaccg gagcgtcttc
    11461 actgcgcacc gcttccgtct gcggcgtcag ttggatcact gcccctttta gcatcacctg
    11521 cttaacgctc agttgatgac tcagtagtgg taaaagcgcc acgtccagac gcatgttgtc
    11581 ggcgcgaacc agtggctggc ttgcgccctg ggcggtgaga gacattcgcc cggagaggat
    11641 actaagctgc ggccagacgt gccaacgcag tggcccgtcg agctgcaatt gataaccgct
    11701 acgtgcagca acttgcttga ccatatagtc gcggaaatca ttcggattca ccagcaacac
    11761 taacgcagat aacccggcca ccagcacgac caggagtatc atcagcgtcg tcagaaatcg
    11821 tctcatggta tcctcaatgg gcctgaatta gtctttatcg attcggctgg ctaccgcgcc
    11881 ctgctggttg cgatatttcg catcttcacg gcggttgtaa ggtcgcgccg ccgggccgga
    11941 aagcggctca aagctcagcg caccaattaa catgcccgga cgcagcgcca gcggcagctt
    12001 accggagttg tagaactcca gcacaatgca accagaccag cccggatcga tacggtgcgc
    12061 ggtgacgtgc accatcagcc ccagacgcgc caaagaggaa cgcccgtcca gccagcccac
    12121 cagatcggct ggcagcgtca ccgactccag cgtcaccgcc agcgccagct ctcctgggtg
    12181 aagatagaac gcctcgccct cgtcgagaac gatctcatcg ctcatcacgc ggtcaagcgc
    12241 ggcgctcact tcatctttgg gaccgctcag atcgataaac gctgccgtgt gaccacggaa
    12301 ggtacgaaat ttattgccca ggcgtacatc caccgtcgcg ccgttaatac gctccactgg
    12361 cggacgtggg ttgatcgaca aacggccttc atcaagccag gcttcaatat ctcggtcaca
    12421 cagacgcatg gcattttctc ctttcgcgca tcactccctt aacgccaatt acgtcaaggg
    12481 cataccagct tatcactgaa cggtacacaa tttatcaagc ttattcaaag aactgactta
    12541 ttttcgcttt caatatatcg atcgcgatgc ggtttttccc gccgcgcggc acgataatgt
    12601 ccgcgtattg tttagaaggc tcaatgaatt gcaggaacat cgggcgcacg gttttttgat
    12661 attgcgccat cacggaatcc attgaacgcc cacgctcgtt aacgtcacgc ttgatgcggc
    12721 gcatcaggca gatatccagc ggggtatcaa cgaaaatgga gaagttaagt tcgtcacgca
    12781 aacgcgcatc cgtcagcaac aaaatgcctt cgagaatgat gaccttcttc ggctcaacct
    12841 tcaccgtttc tttcatacgc gtatgttcaa catagctgta aaccggcagg tcaattgccg
    12901 agccgcgttt caacgcttgt aaatgctcaa gcaacaggct gtgatccatc gcgctgggat
    12961 ggtcgtagtt ggtcttaacg cgttcttcca tcgacagatg gctttgatct ttgtaatagc
    13021 agtcttcggg aattacgccg atgtgttcat caccgacttg ctcacgtaat tcacgataaa
    13081 gggtactggc aataagactc ttgccggaag ccgatgcgcc agcgataccg ataatgacgc
    13141 actgatgaga ctgatcagtc atatatttag cgacctgatt aacctggatg ttaggaaggg
    13201 ggcgacgaag cgccaaacgc ggcaattata gggatttcat ccgtctgata ccagtcgaat
    13261 agcgttgccg cgcgctcaga gttaattgtt gacaaagaat tcccggtggc aaattacgtt
    13321 gatcagtttt atgtaaggta aaaaatgtta tacgcagttg cgcaaattat ccgcctttac
    13381 gtcactttat gagcaattcg catataaaat gtaaaacttt tgtactagca taaacacaga
    13441 aacgaatact ggcgacctgg ccttgcggat aaagcggtaa taagcaaaca atcacagcat
    13501 gtattaattg ccctgcccca cccgctgctt cacctggtca gtttaggttt agtctcgttt
    13561 atctttaccc ttgtctcgct tgagctttcg cagtttggca cccaactcgc cccactgtgg
    13621 ttcccgacgt ccatcatgat ggtggcgttt tatcgccatg ccgggcgcat gtggccggga
    13681 attgcgctga gctgttcgct gggaaatatc gccgcatcca tcctgctttt ttccaccagc
    13741 tcgctgaaca tgacctggac gaccatcaat attgttgaag ccgtggtcgg ggcagtgctg
    13801 ctacgtaaat tgctgccgtg gtataacccc ttgcaaaatc tggctgactg gctgcgtctg
    13861 gcattcggca gcgccattgt tccgcctctg ttggggggtg cccctattaa gttgtcaagc
    13921 atgttatgac ccctgcgggg tataaaaagt cccgtcgcgc atcatggcga acagaacgtc
    13981 gcagcgtcgt ctcgccaggg cgataagcac ctgattgtgt cgttttccct gactcatttt
    14041 gcgggtgtag taagccctgg agagcggatc cctgagcgcg gcgaaggccg acaggaacaa
    14101 cgcccgtttg agagctttat taccccgtcg cgagggatgc tcaccgcgta ttgacgagcc
    14161 gg
//
LOCUS       DAETPZ010000093        14089 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.093, whole genome shotgun sequence.
ACCESSION   DAETPZ010000093 DAETPZ010000000
VERSION     DAETPZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.093"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(119..199)
                     /locus_tag="K0W59_003232"
                     /pseudo
     CDS             complement(119..199)
                     /locus_tag="K0W59_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
     gene            complement(404..1108)
                     /locus_tag="K0W59_003233"
     CDS             complement(404..1108)
                     /locus_tag="K0W59_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBI3024163.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGLGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1245..1697)
                     /gene="moaE"
                     /locus_tag="K0W59_003234"
     CDS             complement(1245..1697)
                     /gene="moaE"
                     /locus_tag="K0W59_003234"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HBI3024164.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            complement(1699..1944)
                     /gene="moaD"
                     /locus_tag="K0W59_003235"
     CDS             complement(1699..1944)
                     /gene="moaD"
                     /locus_tag="K0W59_003235"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HBI3024165.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFLPPVTGG"
     gene            complement(1937..2422)
                     /gene="moaC"
                     /locus_tag="K0W59_003236"
     CDS             complement(1937..2422)
                     /gene="moaC"
                     /locus_tag="K0W59_003236"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBI3024166.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2425..2937)
                     /gene="moaB"
                     /locus_tag="K0W59_003237"
     CDS             complement(2425..2937)
                     /gene="moaB"
                     /locus_tag="K0W59_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HBI3024167.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2959..3948)
                     /gene="moaA"
                     /locus_tag="K0W59_003238"
     CDS             complement(2959..3948)
                     /gene="moaA"
                     /locus_tag="K0W59_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBI3024168.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3936..4080)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4345..5253
                     /gene="yvcK"
                     /locus_tag="K0W59_003239"
     CDS             4345..5253
                     /gene="yvcK"
                     /locus_tag="K0W59_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HBI3024169.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5445..7466)
                     /gene="uvrB"
                     /locus_tag="K0W59_003240"
     CDS             complement(5445..7466)
                     /gene="uvrB"
                     /locus_tag="K0W59_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HBI3024170.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(8045..8722)
                     /gene="bioD"
                     /locus_tag="K0W59_003241"
     CDS             complement(8045..8722)
                     /gene="bioD"
                     /locus_tag="K0W59_003241"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBI3024171.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPSSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QVIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8715..9470)
                     /gene="bioC"
                     /locus_tag="K0W59_003242"
     CDS             complement(8715..9470)
                     /gene="bioC"
                     /locus_tag="K0W59_003242"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HBI3024172.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9457..10611)
                     /gene="bioF"
                     /locus_tag="K0W59_003243"
     CDS             complement(9457..10611)
                     /gene="bioF"
                     /locus_tag="K0W59_003243"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HBI3024173.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIACFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10608..11648)
                     /gene="bioB"
                     /locus_tag="K0W59_003244"
     CDS             complement(10608..11648)
                     /gene="bioB"
                     /locus_tag="K0W59_003244"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBI3024174.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            11735..13024
                     /gene="bioA"
                     /locus_tag="K0W59_003245"
     CDS             11735..13024
                     /gene="bioA"
                     /locus_tag="K0W59_003245"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HBI3024175.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            13002..13472
                     /locus_tag="K0W59_003246"
     CDS             13002..13472
                     /locus_tag="K0W59_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HBI3024176.1"
                     /translation="MKHFFANNGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTKSF
                     VVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTGYD
                     GAAPPKGETHRYIFTVHALDVERIDVDEGGSGAMVGFNVHFHSLASASITAMFS"
BASE COUNT         3294 a         3796 c         3595 g         3404 t
ORIGIN      
        1 ccgttacgca ccacgccttc agtagcggag caggaaggac atctgatgga aatggaagcc
       61 acgcaagcac cttaaaatca ccatcataca ctaaatcagt aagttggcag cattacccgg
      121 taatgagcca tataccgttt aatatcctgg cctcgtgctg aaaatctaat ttattgctgt
      181 tttgcgagta tgattccatt cttttagttg tccttagaaa aaatattaat attcaccatc
      241 aataaattta aaaccacgca tacaaaatac ttttgtattt atggatacgg aaaacgattc
      301 cgctcgctca taaggcatat tttatacctg gtacttgtta agtgtcgttt ttagtaagaa
      361 aaaaatcccg acacatggcc gggatagtga tggtgaaaaa gaattaacgg cggttgccga
      421 agatccgtaa caacatcagg aacaggttga tgaagtccag atacaaggtt aacgcgccaa
      481 gaatggaata tttgcgcagg ttcgacgtgt cgcgggtatc aatctgctca cccatatttt
      541 tcaatttctg agtgtcatac gccgtcaatc cgacaaagac aatcacgccg atgtaggtaa
      601 ctgcccacat caatgcttcg cttttcagcc agaagttgac cagcgatgcc agcacaatgc
      661 cgattaacgc cataaacagc atattgccca agccacttaa atcgcgcttc gtggtgtaac
      721 cgtacaggct cattgcgccg aacatcccgg cagtaacgac gaaagtactg gcgatagaag
      781 cagcggtata gacaatgaat atactggaaa gcgtaagacc cgtcagcgcc gaataaagca
      841 taaagagcat cgtcgttaca cctgcgctca gcttttgaat catcgctgat aacacaataa
      901 ccaatgctaa ttgcgcgatg atcagaccga ttaaaaagac acggttagtg aacaacagct
      961 ccatcacggc cgcggaatta gccgcatacc aggcaacaaa tgcggtcagc aacaagccaa
     1021 cggtcatcca gccatagact tgcgccatat aagtttgcaa gccagcccgg ggttgtacga
     1081 ttgaatcaga acgtgggaat ctgtccatga cgatctcctg aagatataag gaatatctta
     1141 aggatactgc aaaatgatga ggctgtgcat cgacgcagcg taaacgcatg tactgagcgg
     1201 tgaaattgcc ggacgcagcg gtgccttatc cggctaacaa aaaactacca gcgttttgcc
     1261 gcctgcagat cgctctcccg ggcttcaacc cagcggtcgc cttccggcgt ggcttcgcgc
     1321 ttccagaacg gtgcgcgggt tttgagataa tccataataa actgcccggc ttcaaacgca
     1381 ctgctgcgat gcgcactggt gacaccgaca aaaacgattt catcgcccgg ccataattcc
     1441 ccgatgcggt gaatcaccgt gacgcgcccc agcggccagc ggttacgcgc ttcatcaaca
     1501 atttctgcca gtgctttttc agtcatcccc ggatagtgtt cgagggttaa tgctttgacg
     1561 ctgtcgccca ggttatggtt gcgcaccttt ccggtgaagg tgactaccgc accgtcttcg
     1621 tcacgctccg ccagccacgg gtactcttct cctacgctga acggctgcgg gccgacaaca
     1681 attttggttt ctgccatctt aacctccggt taccggcggg aggaaagcta cttcgtcgcc
     1741 gtcagtcagc ggatggtcaa aactcaccag cgtctggttg acggcagcca gcaatttgcc
     1801 atcttccagc gccagcgccc agcgatcgct ctgcgcagcc aggtgctggc gtaacgcttc
     1861 aacggttggg taatccgcag ccacttcggt tgcatctgtt cccaccaact cgcgcacctg
     1921 ggcgaaaaaa agaactttaa tcatccgctt ccaccttaaa gtcacccgac ttgccgccgc
     1981 tcttcgccag caaacgtacc ggaccaatca ccatatcttt ttgcaccgct ttgcacatgt
     2041 cataaatggt cagcgccgcc acggaggccg cggttaacgc ttccatttcg acaccggttt
     2101 tcccggtcag gcggcataag gtttctatac gcacccgatt gtgctccggc tcggcctgta
     2161 aattgacttc aactttgctg agcatcagcg ggtgacacag cgggatcaga tcccaagtgc
     2221 gttttgccgc ctgaataccg gcaatacgcg cagtggcaaa tacgtcgcct ttgtggtggc
     2281 gaccatcaat aatcatcgcc agcgtctcgc tgcgcatggt gacaaaggct tccgcccgcg
     2341 cttcacgcac ggtttccgct ttggcggaga catccaccat gtgcgcttcg ccagcggcgt
     2401 tgatatgggt cagttgcgac atacttattt cttcaaatgt ggatggaaat tacacggacg
     2461 cgtacgggca tccagctgcg gcgcgatgat attttcccat gcggtacggc acgctttggt
     2521 cgaacccggc atggcgaaaa tcagcgtttt gttagcgacg cccgctaccg cacgagattg
     2581 caacgtggaa gtgccaatct cttcaaacga caacatacgg aacacttcac caaaaccttc
     2641 aacctcacgg tcgaacaacg gcagcagcgc ttcgggagcc tgatcacctt ccgtcaggcc
     2701 agtaccaccc gtaatcaata ccacttgtac atcgtcgctg gcgatccacg cagatacctg
     2761 agcgcgaata gcgtagcggt tttctttcac aatggcttta tcgacaacgt gatggcccgc
     2821 ttcttgcgcc gaatcgcgca gatagtgacc ggaggtatcg tcttcttcac cgcgacgatt
     2881 agaaaccgta agaatagcaa tacgggtcgg gataaattca gtgcttacct gactcatctg
     2941 atctctcctt ttgacgtttt agccgccaat gtacgataag ttttgcgtaa taccggtgtt
     3001 gttttgatgc aggaaatggg tctgtttctt ctcccgcagc gccgctgaaa tacgcgcttc
     3061 cagcgcctgt tgctgggtat cgtcttccag cagatcgcgc aggttaacgc cgccttcacc
     3121 aaacaggcag agatggagtt taccaatgga ggaaacgcgc aggcggttgc aagtggcgca
     3181 gaagtctttt tcatacggca tgataaggcc aatctctccg gcgtaatctg gatggcaaaa
     3241 gacttgcgcg ggaccgtcgc tgcgttgacg taattggtgg atccagccgc gacgcagtag
     3301 ctcgtcacgc agaacctgac cagagatgtg atgcttacgg aagagctcgc tgccctcgcc
     3361 cgtttccatc agttcgatga aacgcagctg gatagggcga tgctggatcc agttcagaaa
     3421 ggtgtcgagc tggtgatgat taacatcacg catcagcacg gtattgactt tgaccttctc
     3481 aaaaccggcc tcaaatgcag catcaatccc tgccatgacc tggttgaatt tatcctgccc
     3541 ggtaatagcg tgaaactggc gggcgtccaa actgtcgaca ctgacgttaa tgccagtaag
     3601 tcccgcatcg cgccagttcg ccacatcgcg ttccagacgg taaccattgg tggtgaccgc
     3661 aatctggcgg atagcgtcgt tttcccgcac agcggcgatg atatcggtaa agtcgcggcg
     3721 taaagacggc tctcctcctg tcaggcgcac tttttccgtg cccaggctgg cgaaggcgcg
     3781 cgtaacccgg cgaatttcat cgacggtaag aaagccttta ttggtgacgc cgctcggttt
     3841 gtagccatcc ggcaggcagt aggtgcaacg aaagttacac acatcggtaa tcgacaggcg
     3901 caagtagtaa aacttacgcg caaatgcatc agtcagttgt gaagccatgt acacctttcc
     3961 agatacggga ggcgaagtca tttcttcctt cgccctggtg gcaatcttta cgagcattgt
     4021 cacggccaaa gcaccgtatc agttgaccca ggtgcagagg ctagagtgtt tagtggttaa
     4081 gccgatacta gcgtgtaaat gagattttta ccatccacac tttcgctatg caatcatgta
     4141 tatagcgtca tgatcgtgct atttcgctac agaataaggg aaaatggcgg ttttactttg
     4201 atatacatca tcagggaggc atagcgtgca tcgtccttta gagatagaag aaaatagttt
     4261 gtatcctcaa tattggcagg ttaattgctg tttccccgca atttgcgcta ctgtagcgcg
     4321 gataatctga ctccaggaaa ctatatgcgc aatcgtacgc tggctgatct tgatcgtgtc
     4381 gttgctctcg gcggagggca tggactggga cgcgttctct catcactttc gtctttgggt
     4441 tctcgtttaa cgggtatcgt caccaccacg gataatggtg gctcgacggg gcgtattcgc
     4501 cgttcagaag gcggcattgc ttggggcgat atgcgcaact gcctcaacca gctgataacg
     4561 gaaccgagcg tcgcctccgc gatgtttgaa taccgttttg gcggcaatgg cgaactttcc
     4621 ggtcataatc tcggaaactt gatgttaaag gcgctggatc accttagcgt gcggcctctg
     4681 gaagccatca atttaattcg taatctgctg aaagtggata cgcatttgat tccaatgtca
     4741 gagcatcctg ttgatctgat ggcgattgac gatcaggggc atgaagttta cggcgaggtc
     4801 aatatcgacc agttaactac gccgattcaa gagttattgt taacgcctaa tgtacccgca
     4861 acgcgtgagg cggttcacgc tatcaatgaa gcggatctca tcattattgg gcctggcagt
     4921 ttttatacca gcctgatgcc aattctgctg ctgaaggaaa tcgcccaggc attacgccgc
     4981 acgccagcgc cgatggttta tatcggcaat ctggggcgtg agttgagttt acctgcggct
     5041 aatttgaagc tggaaagcaa gctggcaatt atggagcagt atgttggtaa aaaggtcatt
     5101 gatgcggtca tcgtcgggcc aaaagtggat gtctcggcgg tgaaagagcg gattgtgatc
     5161 caggaggtac tggaggccag cgatattccg tatcgtcatg accgccagtt gttacataac
     5221 gcgctggaaa aggcgttaca ggctttaggt taacggaaat ccgaaggaaa attccggctt
     5281 cctattgaag acaaagtgcg cgttgtttat gccggatgcg gcgtgaacgc cttatccggc
     5341 ctacaaaccg cgcaaattca atatattgcg gagaaaatgt aggcctgata agcgtagcgc
     5401 atcaggctgt tttccgtttg tcatcagtct tcttcgctat cctgttacga tgcggcaata
     5461 aacaactcac gcaactgatg caactggtca cgaatttgtg ccgcttcttc gaactccaga
     5521 ttctgcgcgt gttgcatcat cagcccttcc agttcatgga ttttctgctg caacgctttc
     5581 ggcgacatat ccatcggcac attatccggc tcaacaatcg ggcgcgattt tcctctgccc
     5641 ttcgctttgg ttttggcaat gttctgcccc agcgccagga tatctaccac tttcttgttc
     5701 aagccttgcg gcgtaatgcc gtgttcctcg ttgtacttct gctgtttctc acggcgacgt
     5761 tcggtttcgc caatcgcttt cgccattgat ggagtgatct tatcgccgta gagaatcgct
     5821 ttaccgttaa cgttacgtgc cgcacgacca atggtctgga tcaacgaacg ttcggaacgc
     5881 aggaagcctt ctttgtcagc gtcgaggatc gccaccagcg acacttccgg catatccaga
     5941 ccttcacgca gtaagttgat ccctaccaat acgtcgaact cacccagacg caagtcgcgg
     6001 ataatctcca tacgctcgac ggtgtcgata tctgagtgaa gataacgcac gcgctcaccg
     6061 tgttcttcga gatattcggt aagatcttcc gccattcgct tggtcagtgt ggtgaccagc
     6121 acgcgttcgt taattgccgc tcgctgacga atctccgaaa gaagatcatc aacctgtgtc
     6181 gccaccggcc gcacttcgat aatcgggtca agcaatccgg ttggacgcac cacctgatcc
     6241 accacatcgc cgccggattt ttccagctcg taattacccg gcgtcgccga aacatagatg
     6301 gtttgcggcg ctaatgcttc gaactcttca aacttaagcg gacggttatc cagcgctgat
     6361 ggcaggcgga agccgtactc caccagtgtc tctttacgcg cccggtcacc gcgatacatg
     6421 ccgccaattt gtggaatggt gacgtgagat tcatcgacga ccagcagccc atcggcaggc
     6481 aggtaatcaa acagcgtcgg cggtggctca cccggtccac gaccggagag gaagcgcgag
     6541 tagttttcaa tccccgaaca gtagcccagc tcgttcatca tctccagatc aaactgggta
     6601 cgctgggtca gccgctgctc ttccagcagt ttgttgtttt ccaacagcac tttgcgtctg
     6661 gcggccagtt cttctttgat ctcctccatc gcctgtacga tgcgctcgcg cggtgtgacg
     6721 tagtgcgttt tcgggtagat ggtaaaacgt ggaatagtgg aaacaatctg cccggtcagc
     6781 gggtcaaata acgacaatcg ttccacttcc tcgtcaaaca gttccacgcg aagtgcaatg
     6841 tcatccgatt ctgccgggaa gatatctatc acctcgccac gaacgcggaa agtaccacgc
     6901 tggaatgctt gatcattacg agcgtattgc agctccgcca gtcggcgcag aatcgcgcgc
     6961 tgatcgataa tcataccgac cgtgagatgg agcatcatct tgagatataa atcaggatcg
     7021 cccagaccat aaatcgcgga aacagacgcc actacaatca catcacgccg ctccagcatc
     7081 gctttggtgg cggacaaacg catctgctca atatgttcgt taaccgaggc atctttctca
     7141 atgaaggtgt cggaactcgg tacataggct tccggctgat agtagtcgta gtaggagacg
     7201 aaatactcca ccgcgttttc cgggaagaac tctttcattt cgccatacag ctgggccgcc
     7261 agcgttttgt tgggcgcaag taccatggtt gggcgctgaa ggtcagcaat gacattggca
     7321 atggtgaagg ttttcccgga gccagtcacg ccaagtaacg tctggtgcgc caggccatct
     7381 tccagcccct cttcgagacg tcgaatcgcc tctggctgat cgccagaagg tttaaaagcg
     7441 gaattcagtt tgaacggttt actcatgagt cgctacctga aggagttggg cgggcaggta
     7501 tgtaatttta ctcgtcgtac ttaattatgc caacaaatta tactggataa aaaaacagtt
     7561 catcaccata atatttctga tacagcgtaa actccgctgt caatcatgag caaaatttac
     7621 tctgtggcga gataaaactc cggccttacc gggttatccc caaagcaacg gcttttttaa
     7681 catttgtcaa gatgagtgat gacagtttta tggcaagaga tgcctgtaca gtgactgcca
     7741 ttgcatttat ctaaccagtt aaaaaataaa agatatttct ttgagccgtc tttaacgcca
     7801 attcgcgtgc aagccgcgta ttctctcgct tgcctcgtgt tttctaactc taatacacat
     7861 ggttatccac aggaatagtg gataactgtc tccagcccct atcccccgcc gcctgggcaa
     7921 ttcccacatt cgccgcatga ggcggaagta aaattttttt cgtgattatt ttagaattta
     7981 attggttaaa ttgcagtcaa tcgaagacgc gatctcgctc gcaatttaac caaatacaga
     8041 atggctacaa caaggcaagg tttatgtact ttccggttgc cgcattttct ggattttctg
     8101 caagccaggg gatctctccc agcagcggcg cgggaatcat gcgggtgagc gtggtcatat
     8161 attcagcgtg acgttttccc ggaggcgtaa catcgttcgc cacccaaccc gccagagtca
     8221 gtccggcgtg ttgtattacc tgcgcagtca acatcgcgtg attaatacag ccgagtttca
     8281 caccaactac cagtatcacc ggcagttgtt cctgtgttac ccaatctgca aaagtgaaag
     8341 tgtcagaaag cggcgtaaac cagccgccag caccttccac taacacccag tcagcctgtt
     8401 gttcaagcgc gcgtaatccg gcgctcatta ccaatgattc tatcggcctg ccctcttggg
     8461 cgctgatgat gtgcggcgag gagggttctg cgaaggtgta aggatttacc gttgcgtaat
     8521 ccagctgcag gctgctgttg tgctgtaacg ccagcgcgtc actattacgt aagccttccg
     8581 gggtcttttc gctgccagag gcgaccggtt tataacctgc cgtccgataa cctactgcct
     8641 tcgcggcttg taaaagtgca caactggcga cggttttccc cacttcggta tccgttccgg
     8701 tgacaaaata acgtttactc acgagcaatc actcccaaaa aaagatgata cgtcagagga
     8761 tatcgcccct gttgttgcgg ccaggccagt tgcaatcgct gcaactgcga acgcgttaat
     8821 attcgcgggt cgcgcccttc atgaagatgc gtagcaccga tgcctttcag cgaacgcatg
     8881 gcactgagcg catcatcaaa ccatagtgtg atgagctgaa tatgatgttg ataacgcacg
     8941 ccgttcagcg actcttcgat ttcatctggc ggtaaaaagc gattagcatg cggacgctcg
     9001 tccaccgcct gccgcgcctg atgcagttcg ggtaacgatc cctgcaccag cgtggtaaac
     9061 gcgaccacgc ctccggggcg caccacccga tacagctcgc ggagtgccgt ggataaatta
     9121 ccgcaccact gcaccgcgag attgctccat gcaagatcga acgtcgcagt cgctaacggc
     9181 agggattcga tatctcccgc cagataatga tctgcggcat ctttatggcg ggcttgcgca
     9241 agcattggcg gcgagagatc taacgccgtc acctgcgcgt gacgttcccg ccagtggcgg
     9301 ctcatccagc caggtccaca acccgcgtcc agtacgtggg tgtatttacg ctgtggaagc
     9361 attgccagta aggcgtcagc actctggcgc tgtagatctg catgttgctc atagtgtgcg
     9421 gctgcccgac caaatgccgc tgcaatggct tgtttattaa ccgttgccat gcagcacctc
     9481 cagcagacga tcgatatcct gcatttcatg cgcagcggtt agcgttaagc gcagtcgcgc
     9541 agtaccagcg ggtacggttg gcgggcgaat cgccgtgacc cagcagcctt gctggcgcag
     9601 tttttctgcc agttgtaacg cacggctgtt atcaccgacg atcaatggct ggatggcgct
     9661 gcatgaatca gccagcgtaa acggcaaatc ctgtactccg gcacgaaaac acgcaatgag
     9721 tgacaccagt ttttcgcgcc gtgcatcccc ctcatcacgg cgaatgaccg ccagcgacgc
     9781 acgtaatgcc tgcgcctgag cgggcggcat actggtgctg tagataaggt ggcgggcgaa
     9841 ttgcagcaga taatccgcca ccgtacttga gcaaagcacc gcagccccac tgacgccaaa
     9901 tcctttgcca aaagtcacta ccagcaattc tggttttacc ttttgcagcc agcagctgcc
     9961 gcgcccctgc tccccgataa cgcccgtgcc gtgggcatca tcgaccatca accagccatt
    10021 gtgctgttgc gttacctgct ggatttccgc cagtggcgcg ctatcgccgt ccatgctgaa
    10081 cacgccttct gtcaccacca gttgctgccc cggacagggg gaagcaagca gtcgcgccag
    10141 atgagcgaca tcgttatgag taaaacggcg aagctgcgac gggcttaaac tggcagcctc
    10201 cagcaatgag gcatggctaa gccggtcggc aacaatacgg tcctctttcg ccatcatcgc
    10261 ggcaatgact gcctgattcg cggcaaaacc agagataaac agcagcgccc gcgaatagcc
    10321 aagccactcg gccagttctt cttccagcgc ctgatgcacc acgctataac cgctgacgtg
    10381 accggagccg ccgctaccga tgccaaattg ctccgccccc tgcttccagg cacggataat
    10441 ttgcggatga tggcttaaac ctaaataatc gttactggaa aagttcagat actggcgatc
    10501 atccgccacc agccagcgtc cggctccttg cgccaccgga taacggcgac gcagggcatc
    10561 ggcagcacgc cgcgcatcga gcgccgcgtt gattttctcc tgccagctca taatgctgcc
    10621 gcgttgtaat attcgtcggt gtccggggtc atcagcgcct gtccaagacg ttgctgttgt
    10681 tcgttatccc ctgccagcac ggcagtttgc tgcggattta gccccagttt gcggaacagt
    10741 tgcaggtctt tatcttcttc cggattcggc gtggtcagca gtttgcaacc gtagaaaatc
    10801 gagtttgcgc ctgccataaa gcacatcgcc tgagtctgtt cgttcatctg ctcgcgtccg
    10861 gcagaaaggc gcacgtaaga ggtcggcatc atgatccgcg ctacggcaat ggtgcgaata
    10921 aaatcaaagg catcaacgtc atcgttatcg gcaagcggcg tgcctttcac cttcaccagc
    10981 atgttgattg gtacgctttc cggcggcgtc ggcaggtttg ccagttgcag caataatccg
    11041 gcgcgatctt ttaccgtttc gcctaagccc acaatgccac ctgagcagac tttgatcccg
    11101 gcatcgcgca ctttttccag cgtatcgagg cgttcctgat aagtgcgtgt ggtgatgata
    11161 ttgccgtaaa actccggcga ggtgtccagg ttgtggttgt agtaatccag cccggcgttc
    11221 gcgaggcgct gcgcctgaga ttcactcaac gtgcccagcg tcatacacgc ctccagcccc
    11281 atcgctttta ccccctgcac catttgttcc aggtacggca tatcgcgttc gtggggattc
    11341 ttccatgccg cgcccataca gaagcgcgtc gatcctgccg ctttcgcttt gcgcgccgac
    11401 tccagcacct gttcaacttc catcaaccgc tcggcttcca gcccggtttt gtagcgcgag
    11461 ctttgcgggc agtatttgca atcttccgga caagctccgg tcttaatcga cagcaacgtg
    11521 ctgacctgca cctgacgagg atcgaaatgc tggcgatgca cctgctgcgc ttcaaacagc
    11581 agatccagca acggtttttc aaataattct gtgacttgcg acaatgtcca gcgtgggcgg
    11641 tgagccatgg ggcttctcca aaacgtgttt tttgttgtta attcggtgta ggcttgtaaa
    11701 cctaaatctt ttcaatttgg tttacaagtc gattatgaca acggacgatc ttgcctttga
    11761 ccaacgccat atctggcacc catacacatc catgacctcc cctctgccgg tttatccggt
    11821 ggcgagcgcc gaaggttgcg agctaatttt gtctgacggc agacgcctgg ttgacggtat
    11881 gtcgtcctgg tgggcggcga tccacggcta caatcacccg cagcttaatg cggcgatgaa
    11941 gacgcaaatt gatgccatgt cgcatgtgat gtttggcggt atcacccatg cgcccgccat
    12001 cgagctatgc cgcaaactgg tggcaatgac gccgcaaccg ctggagtgcg tttttctcgc
    12061 ggactccggt tccgtagcgg tggaagtggc gatgaaaatg gcgttgcagt actggcaagc
    12121 caaaggcgaa gcgcgccagc gttttctgac cttccgcaat ggttatcatg gcgatacctt
    12181 tggcgcgatg tcggtgtgcg atccggacaa ctcaatgcac agcctgtgga aaggctatct
    12241 gccagaaaac ttgtttgctc ccgccccgca aagccgcatg gatggtgaat gggatgaacg
    12301 cgatatggtt ggttttgccc gcctgatggc ggcgcatcgt catgaaatcg cagcggtgat
    12361 cattgagccg attgtccagg gcgcaggcgg gatgcgcatg taccatccgg aatggttaaa
    12421 acgaatccgc aaaatgtgcg atcgcgaagg tatcttgctg attgccgacg agatcgccac
    12481 cggatttggt cgtaccggca aactgtttgc ctgtgaatat gcagaaatcg cgccggacat
    12541 tttgtgcctc ggtaaagcct taaccggcgg cacaatgacc ctttccgcca cacttaccac
    12601 gcgcgaggtt gcagaaacca tcagtaacgg cgaagccggc tgctttatgc atgggccaac
    12661 ttttatgggc aatccgctgg cctgcgcggc agcaaacgcc agcctggcga ttatcgaatc
    12721 cggcgaatgg cagcagcagg tggcggctat tgaagtgcag ctgcgcgagc aactggcacc
    12781 agcccgtgat gccgaaatgg ttgccgatgt gcgcgtactg ggggcaatcg gtgtggtcga
    12841 aaccactcgt ccggtgaata tggcggcgct gcaaaaattc tttgtcgaac agggggtatg
    12901 gatccggcct tttggcaaac tgatttacct gatgccgccc tatattattc tcccgcaaca
    12961 gttgcagcgt ctgaccgcag cggttaaccg cgcggtacag gatgaaacat ttttttgcca
    13021 ataatggcga taagttgccg catcgtcatg tctttaacgg catgggttac gatggcgata
    13081 atatttcacc gcatctggcg tgggatgatg ttcctgcggg aacgaaaagt tttgttgtca
    13141 cctgctacga cccggatgcg ccaaccggct ccggctggtg gcactgggta gttgttaatt
    13201 tacccgctga tacccgcgta ttaccgcaag ggtttggctc tggtctggta gcaatgccag
    13261 acggcgtttt gcagacgcgt accgattttg gtaaaaccgg atacgatggc gcagcgccgc
    13321 cgaaaggcga aacccatcgc tacattttta ccgttcacgc gctggatgta gagcgtattg
    13381 atgtcgatga aggaggcagc ggcgcgatgg tcgggtttaa cgttcatttc cactctctgg
    13441 caagcgcatc gattaccgcg atgtttagtt aatcactctg ccagatggcg caatgccatc
    13501 tggtatcact taaaggtatt aaaaacaact ttttgtcttt ttaccttccc gtttcgctca
    13561 agttagtata aaaaagctga acgagaaaca taaaacgctg taaatatcaa tatgttatga
    13621 aagatttagt ctaaataata gactgcataa tactacaaaa cacaacatat ccagtcacta
    13681 tgaatcaact acttagatag tattagtgac ctgaggcaga gcattagcgc aaggtgattt
    13741 tttgtcctct tgcgctaatt ttttgtcaac ggactgggtt agttgaattt acaaccatgc
    13801 taagtatctc gataagcgca gagaaatgat gcagtggtgg gcggattggc ttgatgagaa
    13861 ggttaggtag gcaaatccat gcctgagcag agccttgccc tctacctgag cccatgctca
    13921 ttctttggcc tcaacgcgag catgacctca ctggctggac ccaaaatgga ctatgacaga
    13981 aagatctgat atttttcctc gcaaaaactt agaggcttta ttttaatgca taagagcaac
    14041 tgctttgaca cggcaaggct tgtggcagct atgatggttc tggtcagtc
//
LOCUS       DAETPZ010000094        13996 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.094, whole genome shotgun sequence.
ACCESSION   DAETPZ010000094 DAETPZ010000000
VERSION     DAETPZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13996
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.094"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..422
                     /locus_tag="K0W59_003247"
     CDS             <1..422
                     /locus_tag="K0W59_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024177.1"
                     /translation="DGEPGLYAAMENNHPLCVTRFLSKINGIAFKYKLSKANIMDLLK
                     GATAQGTPALYIAMSKGNEDVVLSYISTLGAFAKKHSFSQHQLFTLLAAKNHDNMSAV
                     HIAIHHKHYKTVETYYAAINVISQSLSFSADEIKTYL"
     gene            594..701
                     /locus_tag="K0W59_003248"
     CDS             594..701
                     /locus_tag="K0W59_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024178.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(754..1508)
                     /locus_tag="K0W59_003249"
                     /pseudo
     CDS             complement(754..1508)
                     /locus_tag="K0W59_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
     gene            1794..2723
                     /gene="pfkB"
                     /locus_tag="K0W59_003250"
     CDS             1794..2723
                     /gene="pfkB"
                     /locus_tag="K0W59_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HBI3024179.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVSRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            2824..3114
                     /gene="ghoS"
                     /locus_tag="K0W59_003251"
     CDS             2824..3114
                     /gene="ghoS"
                     /locus_tag="K0W59_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBI3024180.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3220..4080
                     /locus_tag="K0W59_003252"
     CDS             3220..4080
                     /locus_tag="K0W59_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBI3024181.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4121..4657)
                     /locus_tag="K0W59_003253"
     CDS             complement(4121..4657)
                     /locus_tag="K0W59_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HBI3024182.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            4804..5471
                     /gene="hxpB"
                     /locus_tag="K0W59_003254"
                     /pseudo
     CDS             4804..5471
                     /gene="hxpB"
                     /locus_tag="K0W59_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
     gene            5634..6224
                     /locus_tag="K0W59_003255"
     CDS             5634..6224
                     /locus_tag="K0W59_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBI3024183.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6357..7747
                     /gene="tcyP"
                     /locus_tag="K0W59_003256"
                     /pseudo
     CDS             6357..7747
                     /gene="tcyP"
                     /locus_tag="K0W59_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(7751..8566)
                     /locus_tag="K0W59_003257"
     CDS             complement(7751..8566)
                     /locus_tag="K0W59_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024184.1"
                     /translation="MRREMFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFIS
                     TGQCDCDEIAESNFNRMIAYMSNINYDASKLGAPALSFDTYLQDNVKYRVIINNLYGS
                     EFRIRDINEDFVGMDFTCEFRPEKMTNLINIKNRLVIHYLRTIYYEKNYIHPAGSIFA
                     AIQKNESLLKFPSIISMLNINLLFNPLNLPGMGSGVLEDIMSIPDSSLRKRLGYEVLS
                     FSLQAHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(8843..9106)
                     /gene="cedA"
                     /locus_tag="K0W59_003258"
     CDS             complement(8843..9106)
                     /gene="cedA"
                     /locus_tag="K0W59_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HBI3024185.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            9289..11550
                     /gene="katE"
                     /locus_tag="K0W59_003259"
     CDS             9289..11550
                     /gene="katE"
                     /locus_tag="K0W59_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HBI3024186.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPRSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGHVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            complement(11597..12355)
                     /gene="chbG"
                     /locus_tag="K0W59_003260"
     CDS             complement(11597..12355)
                     /gene="chbG"
                     /locus_tag="K0W59_003260"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBI3024187.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVPDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(12368..13720)
                     /gene="celF"
                     /locus_tag="K0W59_003261"
                     /gene_synonym="chbF"
     CDS             complement(12368..13720)
                     /gene="celF"
                     /locus_tag="K0W59_003261"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBI3024188.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RYTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKNVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(13825..>13996)
                     /locus_tag="K0W59_003262"
     CDS             complement(13825..>13996)
                     /locus_tag="K0W59_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3024189.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
BASE COUNT         3714 a         3453 c         3218 g         3611 t
ORIGIN      
        1 atgatggtga gccaggatta tacgccgcaa tggaaaataa tcaccctttg tgtgtcacac
       61 ggttcctctc taaaattaat ggcatcgcct ttaaatacaa gttgagcaaa gctaacatca
      121 tggatttatt aaaaggagct acagcacagg gaacccctgc attatacatc gccatgagca
      181 agggtaatga agacgtcgtg ttatcttata tatcgacgct gggtgctttt gcaaaaaaac
      241 attcttttag tcaacatcag ttatttacac tattggccgc taaaaatcat gacaacatgt
      301 cagctgttca tatagccatt catcataagc attataaaac tgtagaaaca tattatgctg
      361 ctataaatgt catcagccaa agcctgagtt ttagtgctga tgaaataaag acgtatttat
      421 aaccagctat ttacgatata tatcgataca taattttatt tttttcataa aacgcagaaa
      481 cagtgaccat gtgcacaacg ccacccgacg gctgtaataa caaaaaatcc cttgatgcct
      541 gtcccttttg ttacactccg ttatcacgca caagagatat gcaggacact ggtatgccga
      601 ctaaacgctt tgataaaaaa cactggaaga tggtggtggt gctactggca atctgtggcg
      661 ctatgttgtt gctacgttgg gcagcaatga tttggggctg agcagtgaca aacgaagaca
      721 gccagaccgc ataacacggt ccggcagata attttacatg ctgtagccca gcgataaggt
      781 tgtccgacgg tcggtatgtt ccggtgcaga ctctggaggt tcagagttcc atgtgacgtt
      841 gtaagccacc ttcagtccaa aatgttcatt aatagcaaca tttaatgcgc tttcggagtt
      901 cagcgttgtg tcttccgcgc caaagaccga aacaccctgc gtaaatttag cgttgtcagt
      961 caactgccat gcataagccc cggaggcata gcccagcggc tgggtttcac tggcattgtc
     1021 ggtatatttg tcgtaacgca cacctggacc gaattcaaag cggaaactgt gtaccgggcc
     1081 attcaaaaac tgacgaccat aacccgcggt caacacatcg cgctctcgat agccgttata
     1141 acggtctgtc agccagcttg cctgaccaaa taaatagtca taatcagtta aattaaagcg
     1201 gctacgcccg ccgcatattt ttctgaagaa cgctcatcat tagaagaagt attactggcg
     1261 ttcccccaca gcgaccaggc agtggtttgt ccataccagg tcatggtggt gtcagccgta
     1321 agtgaggagc ttttcgtatt gcctgattgt gcaagatatc ctgcgttcag attaccttcg
     1381 aaaggttttt tagcgctggc aggatcgtcc atgacagtaa aaacggaatc atcggcagct
     1441 gcattcagtg acgcaaacat gccccccgcc aacataacga tggcaggaac tgtcttcaaa
     1501 agcttcattt atcaagagtc cgtacaacaa aaaagagacc atcacggtcc cggaaacttt
     1561 cttaaggatc aaagattagc gtccctggaa aggtaacgaa ttataaaaag gcgcgaataa
     1621 cttagcaatg tattcttatt tcattttttg aataagcatg tggcgaaaac agatttttat
     1681 ttatatatat ttatctgcaa aattttaaat aaagctctaa taaatcatat tgttaatttc
     1741 ttcactttcc gctgattcgg tgccagactg aaatcagcct ataggaggaa atgatggtac
     1801 gtatctatac gttgacactt gcgccctctc tcgatagcgc aacaattacc ccgcaaattt
     1861 atcccgaagg aaaactgcgc tgtaccgcac cggtgttcga acccgggggc ggcggcatca
     1921 acgtcgcccg cgccattgcc catcttggag gcagtgccac agcgatcttc ccggcgggtg
     1981 gcgcgaccgg cgaacacctg gtttcactgt tggcggatga aaatgtcccc gtcgctactg
     2041 tagaagccaa agactggacc cggcagaatt tacacgtaca tgtggaagca agcggtgaac
     2101 agtatcgttt tgttatgcca ggcgcggcat taaatgaaga tgagtttcgc cagcttgaag
     2161 agcaagttct ggaaattgaa tccggggcca tcctggtcat aagcggaagc ctgccgccag
     2221 gtgtgaagct ggaaaaatta acccaactga tttccgctgc gcaaaaccaa gggatccgct
     2281 gcatcatcga cagttctggc gaagcgttaa gtgcagcact ggcgattggt aacatcgagt
     2341 tggttaagcc taaccaaaaa gaactcagtg cgctggtgag tcgcgaactc acccagccag
     2401 acgatgtccg caaagccgcg caggaaatcg ttaatagcgg caaggccaaa cgggttgtcg
     2461 tttcccttgg tccacaagga gcgctgggtg ttgatagtga aaactgcatt caggtggtgc
     2521 caccaccggt gaaaagccag agtaccgttg gcgctggtga cagcatggtc ggcgcgatga
     2581 cactgaaact ggcagaaaat gcctctcttg aagagatggt ccgttttggc gtagctgcgg
     2641 ggagtgcagc cacactcaat cagggaacac gtctgtgctc ccatgacgat acgcaaaaaa
     2701 tttacgctta cctttcccgc taacaaaaac attcccccag cattggggga atcaccacca
     2761 acctgtcggc aacgcgtttc tccgactatg ctcaaaagtc atgtgataac aaaggggtga
     2821 actatggcca gtggcgatct tgtccgttat gtcataaccg tcatgttgca tgaggataca
     2881 ttgactgaaa ttaacgagtt gaataattac ctgactcgcg acggtttttt gctcaccatg
     2941 acggatgatg agggaaatat ccatgagctg gggactaaca cttttggact tatcagtacc
     3001 caaagtgaag aagaaattag agaactggtt tcggggctta cccaaagtgc aaccggcaaa
     3061 gatcctgaaa tcaccatcac gacctgggag gaatggaata gcaacagaaa ataaatggtt
     3121 tttgggcaat aatcagtctg tggtgtgcgt tagctcgtgt ttttacaccg cattcttgcg
     3181 ctaaccttat gatctggcag acaacatggg agagacatca tgtggcaggc aatcagtcgt
     3241 cttttgagcg agcagttagg tgaaggcgaa atcgaactgc gtaatgaact gcctggcgga
     3301 gaagtccatg ccgcatggca tttgcgctat gcaggacatg actttttcgt caaatgtgat
     3361 gaaagggaac tgcttcccgg ttttaccgcc gaagccgacc aactggagtt actgtcgcgt
     3421 agtaaaaccg tcaccgtgcc taaggtttgg gcagtaggcg ctgaccgtga ctacagtttt
     3481 ctggtgatgg attatctccc acctcgtccg ctggatgcgc atagcgcatt tattcttggt
     3541 cagcaaattg cgcgtttaca tcaatggagt gaccaaccac aatttggcct cgattttgat
     3601 aactcgctct ccacaactcc acagcccaac acctggcaac gtcgctggtc aacgtttttt
     3661 gctgaacaac ggattggctg gcagttggaa ctggcagcag agaaagggat cgctttcggc
     3721 aatatcgacg ccatcgtcga gcatattcag cagcgtctgg cctcgcatca accgcagcct
     3781 tctctgttgc acggcgattt atggtccggc aactgtgcgc tgggtccgga tggcccgtac
     3841 attttcgacc ctgcctgcta ctggggtgac cgagagtgcg acctggcgat gttaccgctg
     3901 catactgaac agccgccaca aatctatgac ggctatcagt cagtatcccc gctacctgcc
     3961 gatttccttg aacgtcaacc ggtttaccaa ctctacacgc tgttaaatcg tgcaagatta
     4021 tttggcggtc agcatttggt tattgctcag cagtcattgg atagattatt agcagcatga
     4081 tatgggttga ggataatggc cgctccgtgc ggccttttga ttaaataaac ccaagcagag
     4141 aaaagaacac ataacctgca gcaatacaaa taaccggcag gatatatagt gagaaaaact
     4201 gcaggaaaat agtatggtgc ggaacaacaa tacgggactc aatttgttca cgcgttagcc
     4261 cctcttcccc tttggctttt tccagaatga gttgatcttc aacaccttcc cgcaagaagc
     4321 gcgcctggcg actcatccgg gcaccggaat cctgcaacgc cagtccgaca aaaatcagga
     4381 tgaaaatcac ccagaacata acgttcacac caccattaaa attaggggtc ggggagttat
     4441 accagaacag attcaaaaaa ggcgtattcg cctgcatcat atcgatcatg acatgagcaa
     4501 agtcgagcat gaccgcatta atgccttcct ggttttcctg ccgggtattc ataaatttca
     4561 gcaaagaaat caatgtagag atcaatgcag gtataaaaat cacccaaccc aaaatccttt
     4621 tcaaaacagc aatgcgtcca gcttgttgat acgtcatgag ttctccttga ttaagacgcg
     4681 tcgtttcact tagtttacct gtagatatct gttttcgccc attctttaaa ggcgatatga
     4741 taggcgctta atcataagca cggcttaata ccttacacat aatgctctaa aggagaggtt
     4801 gtaatgtcaa ccccgcgtca gattcttgct gcaatttttg atatggatgg attacttatc
     4861 gactcagaac ctttatggga tcgagccgaa ctggatgtga tggcaagcct gggggtagat
     4921 atctcccgtc gtaacgagct gccggacacc ttaggtttac gcatcgatat ggtggtcgat
     4981 ctttgtacgc ccggcaaccg tggaatgggc caagccgtca ggaagtagta gaacgggtta
     5041 ttgcccgtgc catttcactg gttgaagaga cacgtccatt attaccaggc gtgcgcgaag
     5101 ctgttgcgtt atgcaaagaa caaggtttat tggtgggact ggcctccgcg tcaccactac
     5161 atatgctgga aaaagtgttg accatgtttg acttacgcga cagtttcgat gccctcgcct
     5221 cggttgaaaa actgccttac agcaagccgc atccgcaagt atatctcgac tgcgcagcaa
     5281 aactgggcgt tgacccactg acctgcgtgg cgctggaaga ctcggtaaat ggcatgatcg
     5341 cttctaaagc agcctgcatg cgttccatcg tcgttcctgc gccagaagcg caaaatgatc
     5401 cacgttttgt attagcaaac gtcaaactct catcgctgac agaactcacc gcaaaagacc
     5461 ttctcggtta atgaccaggg gcagtgatca cgctgcccct ggttctttat ctgaatttgc
     5521 attcaactga cggattaatc gtcaatttaa gagaaagagt tacaccgtca ccacttccgt
     5581 gcactgtata aaaatcctat actgtacgta tcgacagttt agtgagtttt atcatgacgg
     5641 cggaaggtca ccttctcttt tctattgctt gtgcggtatt tgccaaaaat gccgagctga
     5701 cgcccgtgct ggcacagggt gactggtggc atattgtccc ttccgcaatc ctgacgtgtt
     5761 tgttaccgga catcgatcac ccaaagtcgt ttcttgggca gcgattaaaa tggatatcaa
     5821 aaccgatcgc ccgcgctttt gggcatcgtg gttttaccca cagtctgctg gcggtatttg
     5881 cgctgctggc aaccttttac cttaaggttc cggaaggctg gttcattccg gctgatgcgc
     5941 tacaaggaat ggtgctgggt tatttgagcc acatacttgc cgatatgctg acacccgccg
     6001 gtgttcccct gctctggcca tgccgctggc gtttccgctt gcctatcctg gttccccaaa
     6061 agggcaacca actggaacgt tttatctgca tggcattatt tgtctggtcg gtatggatgc
     6121 cccattcatt acccgagaac agcgctgttc gttggtcatc gcaaatgatc aataccttgc
     6181 agatccagtt tcatcggctt attaagcatc aggttgaata ctaaaaaggc aaaaatcacc
     6241 tttctggaat aagcaattcc atttgaatat aagagccagc tcacagttct gttaatcttg
     6301 cgccaacact atgactgcta cgcagtgata gaaataataa gatcaggaga acggggatga
     6361 actttccatt aattgcgaac atcgtggtgt tcgttgtact gctgtttgcg ctggctcaga
     6421 cccgccataa acagtggagt ctggcgaaaa aagtgctggt gggtctggtg atgggtgtgg
     6481 tttttggcct tgccctgcat accatttatg gttctgacag ccaggtactt aaagattctg
     6541 tacagtggtt taacatcgtt ggtaacggca atgttcaact gctgcaaatg atcgttatgc
     6601 cgttagtctt cgcctctatt ctgagcgcgg ttgcccgtct gcataacgca tctcagttag
     6661 gcaaaatcag ttttctgacc atcggtacgc ttttgtttac cacgctgatt gcggcgctgg
     6721 tcggtgtgct ggtcaccacc tgtttggttt gacggctgaa ggtctggttc agggtggtgc
     6781 agaaactgca cgtctgaacg ccattgaaag taactatgtt ggtaaagtct ctgatctgag
     6841 cgttccgcag ctggtcttgt cctttatccc gaaaaacccg tttgccgatc ttaccggagc
     6901 caatccgacg tcaattatca gcgtggtaat ttttgccgca ttcctcggcg tagctgcatt
     6961 gaaactgctg aaggatgatg cgccgaaagg tgaacgcgtc ttaaccgcta tcgataccct
     7021 acaaagctga gtgatgaaac tggttcgcct tgtcatgcag ttgacccctt acggcgttct
     7081 ggctctaatg accaaagtgg ttgcaggttc taacttgcaa gacatcatca aactgggaag
     7141 tttcgttgtc gcgtcctacc tcggtctgct gattatgttt gctgtgcatg gcattctgct
     7201 gggcattaat ggcgtgagtc cgctgaagta cttccgtaag gtatggcctg tgctgacgtt
     7261 tgcctttacc agccgttcca gtgctgcgtc tatcccactg aatgtggaag cacaaacgcg
     7321 tcgtctgggc gttcctgaat ccatcgccag tttcgccgcc tctttcggtg caaccattgg
     7381 tcagaacggc tgcgccggtt tgtatccggc aatgctggcg gtgatggttg cgcctacggt
     7441 tggcattaac ccgctggacc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag
     7501 ttccgcaggc gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc
     7561 tgcgatgggc ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga
     7621 catgggccgt acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca
     7681 gtggctgaag caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca
     7741 ccgttaattc ttatgctggc aacggtccgt tttgtatagg ggccgttgct ttacttttta
     7801 aatgttccat cgcggcgatg catactgact catatttaaa cagatcgtca gcgaaaaaaa
     7861 tgtccaactt atcaatacat tcctggctca atgaatgtgc ctgtagtgag aagcttaaaa
     7921 cctcataccc gagacgtttt ctaagtgaac tatctggaat actcataata tcttcaagca
     7981 caccacttcc cattcccgga agatttagtg gattaaaaag aagattgata ttaagcattg
     8041 atattatcga aggaaacttt aggagactct catttttttg aatcgcagca aaaatgctcc
     8101 cggcaggatg tatataattt ttttcatagt atatagtcct taaataatga ataaccaacc
     8161 tattttttat attgatcaaa ttcgtcattt tttcgggtcg gaattcacag gtaaaatcca
     8221 tcccaacaaa atcctcatta atatctctta tccgaaactc actgccataa agattattaa
     8281 ttatcactct atatttaaca ttgtcctgca gatacgtatc aaaactaagt gctggcgctc
     8341 ctagctttga ggcatcatag ttaatgttag acatataggc aatcatacga ttgaagttcg
     8401 actctgctat ttcatcgcag tcacattgac ccgttgatat aaagaaagtg agtgccgcca
     8461 tggtagactc aagcctttct ttaaaagagt cgaaagccag gcttttaata tttaaatcac
     8521 cataattact ctgtattaag tttgtagaaa acatctcccg cctcatattg ttaacaaaat
     8581 tattatctta tttaaatcta agccatttac aatataagtt taagagcgac gccacaggat
     8641 gaactatcaa aaataactca tcatgattag caaaacttaa ccattttaaa ataaataaac
     8701 aattaaagaa aaaagatcac ttatttatag caatagctcg tcaaaggcag ctttttgtta
     8761 caggtggttt gaatgaatgt agcaacgaaa tgcagaattt caggtcctgt aactcccggc
     8821 aaaaccagga ggtatgtaat ccttactcag tcacttcccc ttcctggcgg atctgatttg
     8881 cccaacgttg ggcagattca ggcacagtaa acgccggtga gcgcagaaat gactctccca
     8941 tcagtacaaa cgcaacatat ttgccacgca gcatccagac atcacgaaac gaatccatct
     9001 ttatcgcatg ttctggcggc gcgggttccg tgcgtgggac atagctaata atctggcggt
     9061 tttgctggcg gagcggtttc ttcattactg gcttcactaa acgcatatta aaaattagaa
     9121 aaactgtagt ttagccgatt tagcccctgt acgtcccgct ttgcgtgtat ttcataacac
     9181 cgtttccaga atagtctccg aagcgggatc tggctggtgg tctatagtta gagagttttt
     9241 tgaccaaaaa agcggccctt ccagtaataa attaaggaga cgagttcaat gtcgcaacat
     9301 aacgaaaaga acccacatca gcaccagtca ccactacacg attccagcga agcgaaaccg
     9361 gggatggact cactggcacc tgaggacgga tctcatcgtc cagcggctga accaacaccg
     9421 ccaggtgcac aacctaccgc ccctaggagc ctgaaagccc ctgatacacg taacgaaaaa
     9481 cttaattctc tggaagacgt acgcaaaggc agtgaaaatt atgcgctgac cactaatcag
     9541 ggcgtgcgca tcgccgatga tcaaaactca ctgcgtgccg gtaaccgtgg tccaacgctg
     9601 ctggaagatt ttattctgcg cgagaaaatc acccactttg accatgagcg catcccggaa
     9661 cgtattgttc atgcacgcgg atcagcagct cacggttatt tccagccata taaaagctta
     9721 agcgatatca ccaaagcgga tttcctctca gatccgaaca aaatcacccc agtatttgtt
     9781 aggttctcta ccgttcaggg tggcgctggc tctgccgata ccgtgcgtga tatccgtggt
     9841 tttgccacca agttctatac tgaagagggt atttttgacc tcgttggcaa taacacgcca
     9901 atcttcttta tccaggatgc gcataaattc cccgattttg ttcatgcggt aaaaccagaa
     9961 ccgcactggg caattccaca agggcaaagt gcccacgata ctttctggga ttatgtttct
    10021 ctgcaacctg aaactctgca caacgtgatg tgggcgatgt cggatcgcgg catcccccgc
    10081 agttaccgca ccatggaagg cttcggtatt cacaccttcc gcctgattaa tgccgaaggg
    10141 aaggcaacgt ttgtacgttt ccactggaaa ccactggcag gtaaagcctc actcgtttgg
    10201 gatgaagcac aaaaactcac cggacgtgac ccggacttcc accgccgcga gttgtgggaa
    10261 gccattgaag caggcgattt tccggaatac gaactgggct tccagttgat tcctgaagaa
    10321 gatgaattca agttcgactt cgatcttctc gatccaacca aactgatccc ggaagaactg
    10381 gtgcccgttc agcgtgtcgg caaaatggtg ctcaatcgca atccggataa cttctttgcc
    10441 gaaaacgaac aagcagcatt ccatccaggt catattgttc ctggtctgga cttcaccaac
    10501 gatcccctgt tgcagggacg tttgttctcc tataccgata cacaaatcag tcgtcttggt
    10561 gggccgaatt tccatgagat tccgattaac cgcccgacct gcccttacca taatttccag
    10621 cgtgacggca tgcatcgtat ggggatcgac actaacccgg cgaattatga accgaactcg
    10681 atcaacgata actggccgcg cgaaacacca ccggggccga aacgcggcgg ttttgaatca
    10741 taccaggagc gcgtggaagg caataaagtt cgcgagcgca gcccatcgtt tggcgaatat
    10801 tattcccatc cgcgtctgtt ctggctaagt cagacgccat tcgagcagcg ccatattgtc
    10861 gatggtttca gttttgagtt aagcaaagtc gttcgtccgt atattcgtga gcgcgttgtt
    10921 gaccagctgg cgcatattga tctcactctg gcccaggcgg tggcgaaaaa tctcggtatc
    10981 gaactgactg acgaccagct gaatatcacc ccacctccgg acgtcaacgg tctgaaaaag
    11041 gatccatcct taagtttgta cgccattcct gacggtgatg tgaaaggtca cgtggtagcg
    11101 attttactta atgatgaagt gagatcggca gaccttcttg ccattctcaa ggccctgaag
    11161 gccaaaggcg ttcatgccaa actgctctac tcccgaatgg gtgaagtgac tgcggatgac
    11221 ggtacggtgt tgcctatagc cgctaccttt gccggtgcac cttcgctgac ggtcgatgcg
    11281 gtcattgtcc cttgcggcaa tatcgcggat atcgctgaca acggcgatgc caactactat
    11341 ctgatggaag cctacaaaca ccttaaaccg attgcgctgg cgggagacgc gcgcaagttt
    11401 aaagcaacaa tcaaggtagc tgaccagggt gaagaaggga ttgtggaagc tgacagcacc
    11461 gacggtagtt ttatggatga actgttaacg ctgatggcag aacaccgcgt gtggtcacgc
    11521 attcctaaga ttgacaaaat tccggcgtaa atatggataa ggtcacgtcc cctggggagt
    11581 gaccttacca ccttacttac acatcaagat aactacccaa ccgataacca cgctcagcaa
    11641 ttgcatattt caacgacgct gatgtcagca cttccagctc cgttaaccgt gggaaacagt
    11701 aagcactctg acggatagta ttatcgataa acgccggatg acacatcacc tccagcgaag
    11761 gatcaccccg atggcttgcg tcatccggca cttgcaggaa cagtgcctcg ctaatctctt
    11821 caccataaaa cgcgctactg aatccctgcg aactgcgcag attagccggt aaatcaccag
    11881 cgttagatag cggctgacgg tcgatgcgca acgcaatccc ctcttcagcc gcaaacctgg
    11941 caacaatcgg gaaaatctgc gggaacatat gcacatgatg atggctatca agatgcgtag
    12001 gtttgcgtcc aaatagctca atgaaacgca aatactgact ggcaagctcc tgagtaattt
    12061 cttccagcgg taaagcatct tcttctgcca actgccagat ccattttccc agcacaccat
    12121 cgcgggttaa ccccggcata gctgtcagtg gcttgcccat agtaaggaca aagtgcatcc
    12181 ctatggccag actcggttca tcacgactca actgcaccgc atggtcaata gcctgcccat
    12241 tcaccagcgc cgtcgtcgat gtgacaatcc cattgcgaca ggcctcgata atgccgtagt
    12301 tctgaccttt gcttaagcca aaatcatcgg cattaacaat cagtaagcgt tccataatca
    12361 gcctcggtta atgtgctttt ttaagctctg cgatgcagtc ggcaaaattt ggcagccatt
    12421 tctcgtgcgc cagaatcatc tcgcgtgcca gcagctcagc atcgcgatcg gaatgcacca
    12481 acggactaag gtttagcgcc agtaacacat cgttaaattc tccgctaagt gcggcgttgc
    12541 tggcagcaat ctcgaagcct ttaatggtgt gaatcagccc catcacttta tcatcgaaat
    12601 gcgtaatgcg cggatgtggc gtcgcgccat cgcgccccag cttacaggtc atttctaccg
    12661 cccagtctgc cggaatatta tcaatatgcc catgatgcgg gatattaacg taatgttcag
    12721 cttgcttgtc gttgtagata gcgttgatca cttcgcacgc tgcatcagag taataagccc
    12781 caccgcgctg ttccagttct ttcggcttaa ctttcaactc cgggttttta tacagctcaa
    12841 aaagttgttt ctcgactttc tgtactacct gcgctcgtgc gccgcctttg tagtattcgc
    12901 ccatttcaat agccagcatc tctttttgct tgaagtaata aagcaaataa gaacacggca
    12961 acagattcag agaacgaatt aagccttcgc taaatggcag atcgaaaata tttttaacgc
    13021 cagatgcttt taactgcccg gatgccacac catcaagcaa ttcggcaaag cgcgacttgc
    13081 catttaccag cacattctta atgaacacca tatggttgag gccaaacaaa tcgatagata
    13141 aatcatcgct gtctttcagc atcagaacat cgtgaataaa catcttcatg ccgatcggaa
    13201 tattacacac gccgataaag cgtttaaatc cggtataacg ataaacggct tcagtaacca
    13261 ttcccgccgg gttagtgaag ttaatcaccc atgcattcgg acaaagttct tcgacatctt
    13321 ttacgatgtc aaaaatcacc ggaatggtac gcagaccttt aaacaaaccg cccgcgccgt
    13381 tggtttcctg accaagataa ccatgactta atggaatacg ttcatccagt tcacgcgccg
    13441 gtaattggcc aacgcgcagt tgggtagtaa cgaaatcagc atctttcaat gcttcgcggc
    13501 aatccagcgt tttataaagc ttcatcggga cgccagcgtt atcaatcatc cgttggcaga
    13561 gatcgaaaat aatatccagt ttagctttgc caccctcgac atccaccagc cataattcgc
    13621 tgaccggcaa ttcgtgataa cgcttaataa atccttccag taactccggg gtatagctgc
    13681 tcccgccacc aatagtgacg acttttaatt tctggctcat aatttctccc ttcagtacag
    13741 aatactgata tctggcatat ctgccccccc ggacataaat aatccagcaa caggacagat
    13801 atgtgaattg tcaggtataa cgacttactg attaaattcc gtcaatttct tacgatatct
    13861 cttaggcgta aaggacgtta atttcttaaa cgttttaata aacaaactcg ggctactata
    13921 accggcctca aacgcaatat ccgtcacgga atagttggtc atttccagtt gttttttggc
    13981 aaaattaata cggatc
//
LOCUS       DAETPZ010000095        13988 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.095, whole genome shotgun sequence.
ACCESSION   DAETPZ010000095 DAETPZ010000000
VERSION     DAETPZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13988
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.095"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..454
                     /locus_tag="K0W59_003263"
                     /pseudo
     CDS             1..454
                     /locus_tag="K0W59_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            514..621
                     /locus_tag="K0W59_003264"
     CDS             514..621
                     /locus_tag="K0W59_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HBI3024190.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(602..1333)
                     /gene="yehW"
                     /locus_tag="K0W59_003265"
     CDS             complement(602..1333)
                     /gene="yehW"
                     /locus_tag="K0W59_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HBI3024191.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1338..2264)
                     /gene="yehX"
                     /locus_tag="K0W59_003266"
                     /pseudo
     CDS             complement(1338..2264)
                     /gene="yehX"
                     /locus_tag="K0W59_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
     gene            complement(2257..3415)
                     /gene="yehY"
                     /locus_tag="K0W59_003267"
                     /pseudo
     CDS             complement(2257..3415)
                     /gene="yehY"
                     /locus_tag="K0W59_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
     gene            complement(3422..4339)
                     /gene="osmF"
                     /locus_tag="K0W59_003268"
     CDS             complement(3422..4339)
                     /gene="osmF"
                     /locus_tag="K0W59_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HBI3024192.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDAPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(4591..6888)
                     /gene="bglX"
                     /locus_tag="K0W59_003269"
     CDS             complement(4591..6888)
                     /gene="bglX"
                     /locus_tag="K0W59_003269"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HBI3024193.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MMVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7084..8799
                     /gene="dld"
                     /locus_tag="K0W59_003270"
     CDS             7084..8799
                     /gene="dld"
                     /locus_tag="K0W59_003270"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBI3024194.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(8837..9769)
                     /gene="pbpG"
                     /locus_tag="K0W59_003271"
     CDS             complement(8837..9769)
                     /gene="pbpG"
                     /locus_tag="K0W59_003271"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HBI3024195.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9943..10530)
                     /locus_tag="K0W59_003272"
     CDS             complement(9943..10530)
                     /locus_tag="K0W59_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HBI3024196.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            10700..11278
                     /locus_tag="K0W59_003273"
     CDS             10700..11278
                     /locus_tag="K0W59_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI3024197.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(11395..12156)
                     /locus_tag="K0W59_003274"
                     /pseudo
     CDS             complement(11395..12156)
                     /locus_tag="K0W59_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(12209..13645)
                     /gene="mdtQ"
                     /locus_tag="K0W59_003275"
     CDS             complement(12209..13645)
                     /gene="mdtQ"
                     /locus_tag="K0W59_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HBI3024198.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGMETDINASKTRQQLNDVVGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIRLTGALGGGYKR"
     gene            13869..13952
                     /gene="yohP"
                     /locus_tag="K0W59_003276"
     CDS             13869..13952
                     /gene="yohP"
                     /locus_tag="K0W59_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HBI3024199.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3333 a         3905 c         3527 g         3223 t
ORIGIN      
        1 ggcaatctac atagcctgcg aacgtggatc aaagagcgcg gtcaggatta ccccgcccag
       61 acactcacca cacatctgtt tattcctctg cgccgacggc ttcagtgcca acaaccgact
      121 ctccaggcgc tgctggcgat cctcgacggc gtactgatca actacatcgc catttgtctg
      181 gcttcggcac gtaaaaaaca gggtaaagat gcgctggtgg ttggctggaa tattcaggat
      241 accacccgtc tgtggctgga gggctggatt gccagtcaac aaggatggcg cattgatgtc
      301 ctcgctcgcc cactcgctca atcaactacg ccctgaacta ttcgaaggcc gtacattgct
      361 ggtgtggtgc ggtgaaaatc gaacctccgc ccaacagcag caactcacca gttggcaaga
      421 acaaggccat gatattttcc cactcggtat ttaatgattc gttaacaaat gcgctttact
      481 gtacaatcct ttcgttaaca taaggagtgc attatgcgca tagctaaaat tggggtcatc
      541 gccctgttcc tgtttatggc gttaggcgga attggtggcg tcatgctcgc aggttatacc
      601 tttattttgc gtgctggcta agcgcctgca ccagcctttc aaacaggcgg tctgcgatga
      661 tcgccgccag tgccaccagt aacgccccct ggatcacata cgcggtatta aatccgctaa
      721 gcccgatgat gatgggcgta cccagcgtac tggcccctac cgttgaggcg atcgtcgccg
      781 taccaatgtt gataatcacc gaagttcgca cgcccgccag aatcaccgga gccgccagcg
      841 gtagctcgac cttacgcagt cgctgacctc gactcattcc catacctttc gcaacttctg
      901 tcacgctggg atcaatcgct cccagcccgg caagtgtcac ctgcaggatg ggcagcacac
      961 cgtaaaggat caaggcgata atcgctggtt gcagaccaaa gccgatcacc ggaacggcaa
     1021 tcgccagcac tgcgacaggc ggaaaagtct gtccaacggc ggcaatagtt tccaccagtg
     1081 ggcgaaattc cgcgccccac gggcgagtaa cagcaattcc ggcacctgtg ccaatgatca
     1141 ccgcaaacaa actcgaaatt cccaccagcc agaaatgagc cagtgccaga gctgcaaaac
     1201 tttcttgctg ataaacgggt cgtggcagtt gtgggaacaa ggcagcaaac agcggctggc
     1261 tgtaaggcag ccagaaaatc agcgccacaa aaagagcaat gagccagaac agcggatcgc
     1321 gcaacatctt catacgctta cgcctccacc agcagatcct gaaaatgcag cgtgccgcaa
     1381 ggctggccct gcgtgttcac caccggcagc acctcgcatc cccgcgcgac aaacagggag
     1441 agcgcatcgc gtagcgtcat ctcttctgcc agtgcctcac cttctgcccg ttcttcgcga
     1501 cgcacgtaat ccgccacgct acgtaacgaa agcaggtgcc cccccagttc actacgtcca
     1561 aaaaactggc ggacaaaatc attcgccgga cgagtcagca tcgtcagcgg attcccctgc
     1621 tgcaccactt caccgtgatc catcaatacc agatgttctg ccagccgtag cgcctcatca
     1681 atatcatgag tgaccagcac aatagtgcgc cccagcaaac ggtgaatgcg cgtcatctct
     1741 tgttgcaacg cgccgcgcgt taccgggtcc agtgcgccaa aaggttcatc catcagtaag
     1801 acttgcggat cggcagccag tgcacgcgcc actcccacac gttgctactg accaccggaa
     1861 agctgatgcg gataacgctc acgcaaattt gactccagcc ccagtagcgc cattaattcg
     1921 tcgatacgat cgtcaatccg cgcccgcgac catttttgta attgcggcac ggtagcgatg
     1981 ttttgcgcca cgctccagtg ggggaacagg ccaatagatt gaatggcata gcccatccgg
     2041 cggcgcaact ccagtactgg cagcgagcga atttcttctc cggcaaagcg gatcactccg
     2101 ctgtcatgct ccaccaggcg gttaatcatt ttcagggtgg tggatttgcc ggagccagat
     2161 gtgccaatca gcaccgaaaa actcccttcc tgaaaattga gattgagatc gttaacggct
     2221 ttttgtgcgc cgaacagttt gctgacatgg ctaaattcaa tcattacgtt tcaccttcag
     2281 cagtgcgata agcaaatcga acagcgcgtc ggtaagcacc gccagaacaa ttaccgggat
     2341 cacccccagc aacactaaat caatggcgct gcttagcagc ccctggaaaa ccagcgcacc
     2401 aaaaccgcct gcgccgatta acgccgcaat caccgccata cctacagttt gcaccatcac
     2461 cacccgcagg ctgcgcagaa ataccggtaa cgccagcggt aactgaacat gcaggaaacg
     2521 ctgcgccccg ctcatcccca tcgctctggc gctctccagc acatcgcgcg ggatctggtt
     2581 caaaccgact accacgccgc gcaccagcgg cagcaaggca tagagcacca gcgcaatcag
     2641 tgcgggtgtc attccggttc ctgctatgcc gagcttcccc agccacggaa aggccgtaac
     2701 cagcgcggca agcggcgcaa tcaacaggcc aaagagcgcc accgaaggca cggtctgaat
     2761 gacattgagc agagaaaaaa ttgccccctg ccgcgcagtg gaaaagtagc accagatgcc
     2821 caacggcaca ccaatcacta acgcaggcag caccgcacca aacagcaacg tcagatgttg
     2881 tgccagcgcg tcgtcaaaca catcctgacg gttggcgtat tctttcattt agtgagagat
     2941 cgttaagcgt gccggagtac agcaaccaca gcggaataat ggcaatctgc atatgcaaca
     3001 accagcgcca cagcggatgc gtggagattc ggcggatggc atcgctacag gccagcaatg
     3061 ccagcgccgc cgccagccag aaaccactgc cgaggctggt acgcgccagc gcactgccat
     3121 tttgcgccag ttgggtcgcc gcctttccag ctccccacac caacaatacg aagacgaatt
     3181 gcgccagaat gagtgcaaaa atgctgcctt ttttagcggg gacaaaacag gctgtcagcc
     3241 aggcgcaacc aacgcccacc agcatccaga tcgtttgcgg ccacagttgc cagagatgaa
     3301 gcccttcacc cgagactaaa cgattaggcg cgtaactgat aaacggcagc gccgctgcga
     3361 ttgccgtcag caacaacagc agcgccagaa caggattaat acgtagataa gtcacgggaa
     3421 attacttcgc ccaccctttt tgtttcaggt agtcggcagc cacttttttg gcatccagtc
     3481 cttccactgc aatgctggca ttcagttgct gcaatgtttt tgcatcgagg ctggcgaaga
     3541 ctggctgtag ccactgtgcc atttgcggat actccttcaa caccgattca cgcaccactg
     3601 gtgcaggcgc gtagataggt tgcacgcctt gcggatcgct taaggtttgc agccccagcg
     3661 ccgcgaccgg agcgtcagtg ccgtaagcca ttgcggcatt aacgccagag gtttgctggg
     3721 cagcggcttt aatcgtcacc gccgtgtcgc caccagccag tgacagcaac tgatcctgac
     3781 cgagcttaaa gccataggct ttttcaaacg cgggtaacgc atcggcgcgt tcgataaact
     3841 ctgccgaggc tgccagtttg aaggtgccgc cctcttgcag ataacgactc aggtcggcaa
     3901 gcgaagtgag tttgtttttc tctgccacat cctgacgcac ggcgatagtc caggtgttat
     3961 ttgcaggcgc gggcgtcagc cagattaact tgttgtgctc cgcatcgagc tttttgactt
     4021 tctcgtagcc ctgcccggca tttttccacg ctgcatcgtt ttcatcttta aagaagaaag
     4081 cgccattgcc ggtatattcc ggatagatat ccagttcacc ggaagtaatc gccccgcgca
     4141 ccacaggagt cgttccgagt tgcactttat tcaccgtcgg tacgccgtgg ctttcgagta
     4201 cctgcaagat gatgttgccg agcagcgcgc cttcggtatc gatttttgaa ccgactttaa
     4261 cgggggaagc cgcctgtagc ggcaggctca cggctgccaa cataaccagt gaacctgccc
     4321 agagctttaa gagtggcatg atgctttcct cattctttta ctgttgtttt cagcgaatta
     4381 agagaaaagc atagttgata atgacagagt tagcctgagc ggaaggattc agttgcagaa
     4441 tcagataaat acttaagagg catattcggt ccggcgtttc ctgccggatg cggcgcgagc
     4501 gccttatccg gcctacaaag ggcgaaaatt cagcaagtta agatatgaac atcaggcaaa
     4561 gggcgcaaac gtcgcgccct tactaaagca ttacagcaac tcaaactcgc ctttcttaac
     4621 gcgtgcggaa tcagtgccga taaatacatt gaacttgcca ggctcggcgt catatttcat
     4681 ctgttgattc cagaacttca gcgcctcaat atcgatcggg aagctgacag tctgagtttc
     4741 gcccggtttc agggtgattt tctcaaagcc tttcagctgt ttcacagggc gactcatgga
     4801 agccgtcaca tcctgcaagt acatctgcac taccgtggcc ccctcgcgct taccggtgtt
     4861 cgtcacctgc acgctggccg tcactttgcc gtcacgcttc atggtcggcg cagaaagttt
     4921 cacatcagag acggtgaaag tggtatagct cagaccatag ccgaacggat aaagcgcccc
     4981 gttagcttca tcaaaataac gcgaagtgta tttgttcggc ttgtcggcat tatacggacg
     5041 accggtgttc agatggctgt agtacaccgg gatctgcccg acagaacgcg ggaaggacat
     5101 tggcagcttg ccggacgggt tgtaatcgcc aaacaataca tcggcaattg cattaccgcc
     5161 ttcagtcccc gcaaaccagg tttccagaat cgcatcagcc tgctgatctt ctttcaccag
     5221 cgccagcgga cgcccgttca tcagcaccag caccagcggt ttaccggtgg ctttcagcgc
     5281 cgcaatcaag tcacgttggc cttgcggaat agtgatatcg gtccggctgg aggcttcgtg
     5341 cgccatcccc tgtgcttcac cgactacagc caccaccaca tcagattgtt tcgccgtctg
     5401 caccgcttca tcaatcatct cttgcggcga acgcggatcg actttgaccg cttcttcata
     5461 ctgattcagg aaatcgataa tgcctttgtc actggtaacg ttcgcccctt tggcatacag
     5521 cactttaccg ttttcaccga cggaattttt aatcccggtc agtacggtca cggattgatc
     5581 ggcaacaccg gctgcggacc agctgcccat cacgtcacgt ttactgtccg ccagtggccc
     5641 aaccaccgca atggtggccg attttttcag cggtaacgtt tcgagacggt ttttcagcaa
     5701 cactaggctt tcgcgcgcca cttcacgcgc ttctttacgg tgcaggcggc tttcggcatt
     5761 ggtatccacc gggtcagact ctttcggccc caaatggctg tatgggtcgt taaacaaccc
     5821 catatcatat ttaacgttca gtacatggcg ggcagcatcg tccagctctg ccatggtcac
     5881 tttgccggat ttaatcaacc caggcagata cttcgagtag tactcgtcgc tcatactcat
     5941 gttgattccg gatttcagcg ccacgcgcac cgcatcttcc gggtctgccg ccgtgccatg
     6001 tttaatcagc tctttgattg caccgtgatc ggaaacggtg atgcctttaa agccccactg
     6061 gtcgcgcaga acatctttca gcagccagga atcggaggtg gctggcgtgc cgttcagcga
     6121 gttcagcgcc accatcaccg cgccgctgcc tgcgtccagc cccgctttgt acggcggcat
     6181 ataatcatta aacaggcgct gcggactcat atcgacggtg ttgtactctt taccgccttc
     6241 taccgcgccg tatgcggcaa agtgtttgac gctggtcatc accgagtaac gatctgccgg
     6301 gcttttaccc tgcatcgctt ccaccatggt tttacccatt attgaggtga gatacgtatc
     6361 ttcgccaaaa ccttcggaag cacgtcccca gcgcggatcg cgcgagacat cgaccatcgg
     6421 tgcccaggtc atattcaggc catcatctgc cgcttcataa gcagagacac gcccgaccgt
     6481 tttcaccgca tcgaggttaa aagacgaggc cagaccgagg ctaatcggga acaccgtgcg
     6541 ctgaccgtgc agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc ttaattccat
     6601 cacctgatcc tgcatggcgc ggatatcctg acgggttacg gtgttgaaaa tcgccccaac
     6661 ctgaccgtct ttgatcatct cgcggatcgc ctctttcgga ttatccgggc ctacgctgat
     6721 taagcgcagc tgaccaattt tctcatcaac catcattttc ttgagcagat cggtgacgaa
     6781 cgcatcccgc gcttcaggcg ttaatggatg gttaccaaat aaatcatccg ccagtgctgg
     6841 ctgcaggacc agactcaccg cgattcctac tgaacatagc catttcatat ggatttttct
     6901 tcctttgttg cccgacgtgg cagcgaaaat ggtgcaaaaa ccgtagtttg ccataagcat
     6961 gatggagaga gaaaaagaat gctcagttta ttgtctgaat tttcaaaata ttcactcgct
     7021 gaattgtcat acaaggcgct attctagttt gtgatatttt ttcgccacca caaggagtgg
     7081 aaaatgtctt ccatgacaac aactgataat aaagcctttt tgaatgaact tgcccgtctg
     7141 gtcggtcatt cacacctgct caccgatccc gcaaaaacgg cccgctatcg caagggcttc
     7201 cgttctggtc agggcgacgc gctggctgtc gttttccctg gctcactact ggaattgtgg
     7261 cgggtgctga aagcctgcgt caccgccgac aaaattattc tgatgcaggc tgccaataca
     7321 ggcctgaccg aaggatcgac gccaaacggt aacgactatg atcgcgatat cgtgatcatc
     7381 agcaccctgc gtctcgacaa gctgcacgtt ctcggcaagg gcgaacaggt gctggcctat
     7441 ccgggcacca cgctctattc gctggaaaaa gccctcaaac cgctgggacg cgaaccgcat
     7501 tcggtgattg gatcatcgtg tataggcgca tcggtcatcg gcggtatttg taacaactcc
     7561 ggcggctcgc tggtgcaacg tggcccggcg tataccgaaa tgtcgttatt cgcgcgtata
     7621 gatgaagatg gcaaactgac gctggtgaac catctgggga ttgatctggg cgaaacgccg
     7681 gagcagatcc ttagcaagct ggatgacgat cgcatcaaag atgacgatgt gcgtcacgat
     7741 ggtcgtcacg cccacgatta tgactatgtc caccgcgttc gtgatattga agccgacacg
     7801 cccgcacgtt ataacgccga tccggatcgg ttatttgaat cttctggttg cgccgggaag
     7861 ctggccgtct ttgcggtacg tcttgatacc ttcgaagcgg aaaaaaatca gcaggtgttt
     7921 tatatcggca ccaaccagcc ggaagtgctg accgaaatcc gccgtcatat tctggctaac
     7981 ttcgaaaatc tgccggttgc cggggaatat atgcaccggg atatctacga tattgcggaa
     8041 aaatacggca aagacacctt cctgatgatt gataagttag gcaccgacaa gatgccgttc
     8101 ttctttaatc tcaagggacg caccgatgcg atgctggaga aagtgaaatt cttccgtccg
     8161 cattttactg accgtgcaat gcaaaaattc ggtcacctgt tccccagcca tttaccgccg
     8221 cgcatgaaaa actggcgcga taaatacgag catcatctgc tgttaaaaat ggcaggcgat
     8281 ggcgtcggtg aagccaaatc gtggctggtg gattatttca aacaggccga gggcgatttc
     8341 tttgtctgta cgccggagga aggtagcaaa gcgtttttac accgtttcgc cgctgcgggc
     8401 gcagcaattc gttatcaggc ggtgcattcc gatgaagtcg aagacattct ggcactggat
     8461 atcgctctgc ggcgtaacga caccgagtgg tatgagcatt taccgccgga gatcgacagc
     8521 cagctggtgc acaagctcta ttacggccat tttatgtgct atgtcttcca tcaggattac
     8581 atagtgaaaa aaggcgtgga tgtgcatgcg ttaaaagaac agatgctgga actgctacag
     8641 cagcgcggcg cgcagtaccc tgccgagcat aacgtcggtc atttgtataa agcaccggag
     8701 acgttgcaga agttctatcg cgagaacgat ccgaccaata gcatgaatcc ggggatcggt
     8761 aaaaccagta aacggaaaaa ctggcaggaa gtggagtaaa aattacggat ggcagagtat
     8821 cgccatccga attcacttaa tcgttctgtg ccgtctgccc cgccgccgcc atttgggcgg
     8881 ctttttgttt tttatagctc aacgctgctg ccggcacagg catcacttta ccggtttcaa
     8941 tccaggtacg caggcggctg gcatcggcaa aatgggtata tttgccaaac gcgtccatca
     9001 ctaccagcgc caccggttta ttattgataa ccgtacgcat caccagacaa tggcccgccg
     9061 cattggtaaa gccggttttg gttaactgaa tattccagtt atcgcgatac accagatgat
     9121 tagtattgcg gaacggcagc gtatacgtcg ggttagagaa ggttgccata tcttcccggg
     9181 tagtacttaa ctgcccgatc aacggatatt gtttgctggc aatgagcagt ttggttaagt
     9241 cacgggcagt tgaaacgtta tgcaccgaca atccggtagg ttcaacaaag cgcgtgttgt
     9301 tcattccgag cgatttcgct ttcgcattca ttgccttaat aaaggctttg taaccaccgg
     9361 gataatggtg cgcaaggctt gccgccgcgc ggttttctga agacatcagc gccagcaaca
     9421 gcatatcttt acggctgatt tcgctattca gtcgtacgcg cgaatagacc cctttcatct
     9481 ccggcgtctg gctgatatcc acttttagtt tttcatccag cggcagtcgt gcatccagca
     9541 caaccatcgc ggtcattaat ttgctgatag acgcaatcgg acgcaccaga tccgggtggt
     9601 tcgaatagat cactttgttg gtattcagat caacaatcat cgcgctaccg gaggcaattt
     9661 ccggttgtga agcggtggta gcggctgccg ttttcgcaac ggcctgcggt gcaaaaggca
     9721 cagccagcat cagggccagg ctaaataaag aaactcgaaa tttcggcatg atgagcattc
     9781 agatagtggt tcacgcgcac gggttgcgca ccgccggagt aaggatttac tgaggctagc
     9841 gacgccatca taacgagcaa aaagtgcgat cgtcaaagga gaatcgtgag taaatgctgc
     9901 attgctgaca tttacgccag caatgcaacg tcaaagaact ttctagaaca aacgataacc
     9961 gtagccccat agtataacgg ttagggtgag cagcacttcc agtaccagca cgccaatcgc
    10021 cagcgtcgaa ctggagaagc taaggccttc ctctttgttg atattcagga agctcggaat
    10081 accaaggtaa agcaggtagc cggtgtaaaa cagcgccacc gtgccgacca gcgcacacaa
    10141 ccagaccagt ggataaagcg ccaccagacc gcttaaaaac agcggagttg caacgtagcc
    10201 cgcgaagacc atgcagtgcg caagagacgg acgctgcggg taattgcgcg ccatccacca
    10261 gatgacccgc cccatcaccg caaccccagc cagcataacg ccgtaaaaca agacagccag
    10321 cgccagtccg gtaaaccagg ataacttcag gatagtgcca tcgccaaaat tccagccaat
    10381 ctgtgtagtg ccaatgaagg cgcaaatcac cggaatcgcc gccatcagca aaacgtggtg
    10441 ggtgtaatgg tgagaaatcg tttcgttttc gcgattaatc acctgcattt cacgatcggg
    10501 atgggaaaac agtccccaga catggctcat accgccccct tgttgtgagt tcatgaacct
    10561 gacagttcaa gtataagcca gcttgtgatt attttttgtt catccatgta atttgcaccg
    10621 tctgatgtat gtcctttttc agcgtgaatg attcacaggg tgtatgctta caggcaacca
    10681 aagggagaca gactggccta tggatctcaa tacactcatc tcacaatatg gttatgccgc
    10741 gctggttatc ggtagcctag cggaaggtga aaccgtgact ttgctgggag gcgttgcggc
    10801 acatcagggg ctattaaagt tcccactggt ggtactttct gtggcgcttg gtggcatgat
    10861 tggcgaccag gtgctctatc tgtgcgggcg gcggtttggc ggcaagctgt tacgccgttt
    10921 ctcgaaacat caggataaaa ttgagcgggc gcagaaactt atccaacgcc atccgtatct
    10981 gtttgtcatt ggtacgcgct ttatgtatgg ctttcgggtg attggcccga cgctgattgg
    11041 tgccagccag ctgccgccga aaatctttct gccgctgaat attctcggcg catttgcctg
    11101 ggcgttgatt tttaccacta ttggttacgc tggtggtcag gtgattgcgc cgtggttgca
    11161 caatctcgac cagcatttga agcactgggt ctggttgatt ctggtcgtgg ttctggtggt
    11221 gggcgtgcgc tggtggctga aacggcgcgg gaagaaaaag ccggataatc aggcgtaaag
    11281 cagttgccac agataagcat tcactccaca tccggcaccg ggtgttccat gcctgatgcg
    11341 acgctggtgt cttatctggc ctaacccatt gaaatatcca cgcacctcac tgcgctattc
    11401 tgggttgaac tgcggattcg ccaacataaa gccgccatcc actatcaacg actgccctgt
    11461 cgtgtaattt gcgccctccg aacaaagcca caccaccagg ctggcaatct catgcgttgc
    11521 gccaaaacgc cgtagaggaa tcgaaggctc cgcgtcgggc ttcacgtcgc tatcatccat
    11581 gctgttcatt ggcgtggcta tcgccccagg cgcgactgcg ttcaccaaaa tcttatgcct
    11641 gaccaactcc agcgccatcg ttttggttaa cccgccgagc gcatgtttag cggctgtgta
    11701 ggcgctggca tccggcagcg gcgtatgttc atgtaccgac gtaatgttga tgatgcgacc
    11761 gccctgccct tatttcacca tctgacgagc cgcaatttgc gagcataaaa atgcaccatc
    11821 aacatcaacg gtaaaaatct tgcgccactc atcaaaagcc atatcaagaa acggcgcttt
    11881 ggtcattgca cccgcattat tcaccagcac atcaatacgc ccaaatcgct ggatgagtgt
    11941 ctccagcgcc tgcgcacctt ctggcagatt gccgagatcc agttgcacga tctccgcacg
    12001 tacgccgtgg gtaaccacct cacgcgcggt atcttttgcc ccttcttcat ctgagtgcca
    12061 ggtaatgcca atatcaaacc cctgctgcgc cagtaataac gcgcactctt tgccgatccc
    12121 cgaatcggaa gcggtaatta tcgcgaccta tgccatcgag ttctccactt aacgctgaat
    12181 aaacgttaag tatagaaggc gcatatcatc agcgtttgta acccccgccc agcgcaccgg
    12241 tgagtcgaat ggaggcatcc agccactgcc cttgcaataa caggccattg gcccgctcac
    12301 gcagcgccgg gattctggct tcgctgacgc gggaaccagc aatgatgccc gcgttaaagc
    12361 gggcctgcgc aagacctacc acacgcaagg catcgcgctc aatttgcgcc tgatgctggt
    12421 ttttctccgc cagtgtctga acctgactgg ctgcccgcgc cacgtcattc actgcttcaa
    12481 ccaccgcttt gttgtagctg gcgatagaca agttgctttc ggcttttgcg atatcgagat
    12541 tggcgttaag acgaccacta tcgaaaatag gtagcgtcag gcctgccgta acgcccattt
    12601 gctgcgcgga atgacggaac agatcgctta agtgcaacgc atcctgttgc aggaaggcca
    12661 tcaggttgat gtcaggataa aatgccgctt ttgccgcatc aatagtgctt aacgatgact
    12721 caacgtacca gtgcgccgcc tgcaaatctg cccgccgggc cagtaaggag taccccagtt
    12781 catcaggaag ctggcttgcc actttcggca acgcgaccgg tttaagcttc aatgacttag
    12841 tctggtgatt tgtgagtgca tttaaccgtg cctcaataat tttcattttc cccacgacat
    12901 cgttgagctg ctgccgggtt ttgctggcat taatatcggt ttccataccg tcaactgaag
    12961 aagtaatccc gttctgatat agctggcgat cggtcgcgat aatggtgttc tgctcttttt
    13021 ctatttgctg caagaccgtg tttaacgccg cctgggtttg ccactcccag tacaggcggg
    13081 ctacgctgcc agccagcaat tggcgggttt gctcgcgttc cgccgcccgt gctttaaccg
    13141 tacccaggcg ggcagtaacc tccgcccgat tctttcccca gatatcgaga tgccagcccg
    13201 ccgttaagcc aaaagttccg ttggtgtacc acggaccggt cgtacctgcg gccggatcgt
    13261 tcagagcaaa cggccccatt agcccttctg ccgacatttt ttgccgctcc atatccgccg
    13321 aaaagtcgat ctgcggacca tcctgagtgg caactgcctt cgcctgggct tcagctagct
    13381 gaatgcgctg ttcagccacc tgcatatccg gtgcgttctg tagtgcattg ttaattaatg
    13441 aagtgagctg attatcgtga tactccagcc accattggct gtctggccaa ccatttttca
    13501 gcgccgtggg taatgcggtg tcaacttgtg cagcgggcgt ttgctggctt aacgcctggc
    13561 gggtttcatg cataggcgca cacccggcca gcatcagtaa cagcggaaaa caggcgatgg
    13621 ctggataaaa ggaatcacga ttcatggggg aataatcagg taagaaacgg tgcgcggaga
    13681 ttaccgtgtg ttgcgatata ttttttagtt tcgcgtggca atacatcagt agcaataaaa
    13741 cgacataccc agaaaaatat agactaagta aatgatatct tccgatttat cttaatcgtt
    13801 tatggataac ggcaaagggc ttcgtttttt cctatactta ttcagcactc acaaataaag
    13861 gaacgccaat gaaaattata ctctgggctg tattgattat tttcctgatt gggctactgg
    13921 tggtgactgg cgtatttaag atgatatttt aaaattaatt aatgtcatca gatccgaaaa
    13981 taacgaga
//
LOCUS       DAETPZ010000096        13828 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.096, whole genome shotgun sequence.
ACCESSION   DAETPZ010000096 DAETPZ010000000
VERSION     DAETPZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.096"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..161)
                     /locus_tag="K0W59_003277"
     CDS             complement(<1..161)
                     /locus_tag="K0W59_003277"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024200.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCA"
     gene            246..1748
                     /gene="ycjT"
                     /locus_tag="K0W59_003278"
                     /pseudo
     CDS             246..1748
                     /gene="ycjT"
                     /locus_tag="K0W59_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
     gene            1745..2404
                     /gene="pgmB"
                     /locus_tag="K0W59_003279"
     CDS             1745..2404
                     /gene="pgmB"
                     /locus_tag="K0W59_003279"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBI3024201.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            2418..3500
                     /locus_tag="K0W59_003280"
     CDS             2418..3500
                     /locus_tag="K0W59_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI3024202.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTILEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            3545..4450
                     /gene="ompG"
                     /locus_tag="K0W59_003281"
     CDS             3545..4450
                     /gene="ompG"
                     /locus_tag="K0W59_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HBI3024203.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(4561..5559)
                     /locus_tag="K0W59_003282"
     CDS             complement(4561..5559)
                     /locus_tag="K0W59_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI3024204.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKKSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            5714..7111
                     /locus_tag="K0W59_003283"
     CDS             5714..7111
                     /locus_tag="K0W59_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HBI3024205.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQTTTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            7108..8169
                     /locus_tag="K0W59_003284"
     CDS             7108..8169
                     /locus_tag="K0W59_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HBI3024206.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            8317..9858
                     /gene="tyrR"
                     /locus_tag="K0W59_003285"
     CDS             8317..9858
                     /gene="tyrR"
                     /locus_tag="K0W59_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HBI3024207.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(9902..10408)
                     /gene="tpx"
                     /locus_tag="K0W59_003286"
     CDS             complement(9902..10408)
                     /gene="tpx"
                     /locus_tag="K0W59_003286"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HBI3024208.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGLLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            10527..11486
                     /gene="ycjG"
                     /locus_tag="K0W59_003287"
     CDS             10527..11486
                     /gene="ycjG"
                     /locus_tag="K0W59_003287"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HBI3024209.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALATEARAQGFSLMLGCMLC
                     TSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(11461..12189)
                     /gene="mpaA"
                     /locus_tag="K0W59_003288"
     CDS             complement(11461..12189)
                     /gene="mpaA"
                     /locus_tag="K0W59_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HBI3024210.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(12480..13118)
                     /locus_tag="K0W59_003289"
                     /pseudo
     CDS             complement(12480..13118)
                     /locus_tag="K0W59_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(13136..13717)
                     /locus_tag="K0W59_003290"
                     /pseudo
     CDS             complement(13136..13717)
                     /locus_tag="K0W59_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT         3354 a         3307 c         3739 g         3428 t
ORIGIN      
        1 tctgcgcagc attcaacggc atcgtcaaaa ctatcaaagc gcagaacttc tgcgtcttct
       61 tcgtcaaaaa aatcatcttc gtgaagcttc acgacatagc ggggaagttt gcttctttga
      121 gaggcgggtt tacgtttacg gggtttagct gaacgggcca tataaccacc tgaaagacaa
      181 tgacattgcc tgtttttata acggtaattg cagaccatga caagccgcag ccgtcaggct
      241 gcctactcga aatgtgcgcg caacagagtt acgaccaaca tcttgcagca tcaacagaaa
      301 actggcgtca atggtggcag aaacgtcgta tcacggtaaa tggcggcgat gcgcacgatc
      361 agcaagcgtt agattatgcg ctttatcatc tgcgcatcat gacgccggct cacggcgagc
      421 gcagcagtat tgcggcaaaa ggcttaaccg gcgaaggcta caaaggccac gttttctggg
      481 atacagaagt atttttgctg ccgttccatc tgtttagcga tccgacggtt gcccgaagtt
      541 tactgcgtta tcgctggcac aacttgccag gcgcgcagga gaaagcgcga cgcaacggct
      601 ggcagggcgc gctatttccg tgggaaagcg cgcgcagcgg cgaagaagag acgccggaat
      661 ttgccgccat taacattcgc accgggctgc ggcaaaaagt ggcctcggcg caggcggaac
      721 atcatctggt ggccgatatc gcctgggcgg ttattcaata ctggcagacc acgggggatg
      781 aaagtttcat tgcgcatgaa ggcatggcgc tacttctgga aactgcaaag ttctggatta
      841 gccgcgcggt gagggttaac gaccgtctgg aaattcatga tgttattggg ccagacgaat
      901 ataccgaaca tgtcaataat aacgccttca ccagctatat ggtgtattac aacgtccagc
      961 aggcgctgag tattgcccgc cagtttggct gtagtgacga cgcgtttatt catcgtgccg
     1021 aaatgttcct taaagaactg cggctgccag aaattcagcc cgacggtgtt ttgccgcagg
     1081 atgattcgtt tatgactaag ccggcgatta atctggcgaa atacaaagcg gcggcgggga
     1141 agcaaaccat tctgctggat tattcacgcg cagaagtgaa cgagatgcag atcctcaaac
     1201 aagctgatgt ggtgatgctc aattacatgc tgccagagca gttctcatcg gcatcgtgtc
     1261 ttgccaatct gcaattttat gaaccgcgca ctattcacga ctcgtcatta agtaaagcaa
     1321 tccacggcat tgttgctgca cgctgtggcc tgctgaccca aagttatcag ttctggcgcg
     1381 aggggactga aatcgatctt ggtgctgatc cgcatagttg tgatgatggt atccatgctg
     1441 ccgcaactgg cgctatctgg ctgggggcga ttcagggttt tgccggggtg agcgtgcgtg
     1501 acggtgaatt gcatctaaat ccggcgttac ctgagcagtg gcaacagttg tctttccctc
     1561 tgttctggca gggctgcgaa ttacaggtca ctctcgacgc gcagcgtatt gcgattcgaa
     1621 cttctgcgcc cgtttcactg cgtttgaacg gtcagcttat atccgtggct gaagaatctg
     1681 ttttctgttt gggtgatttt attttgccct tcaatgggac cgctaccacg catcaggagg
     1741 atgaatgaaa ctgcaagggg taattttcga tctggatggt gtaatcaccg ataccgcgca
     1801 tctgcatttc caggcgtggc agcagattgc cgctgaaatt ggcatcagta ttgatgcgca
     1861 gtttaacgaa tctctaaaag ggatcagccg cgatgagtct ctgcggcgca ttctgcaaca
     1921 cgggggcaaa gagggcgact ttaacccgca ggagcgggcg caactggcgt atcgcaaaaa
     1981 tctgctttac gtccactcac tgcgcgagct gacggttaat gctgtgctgc ccggtattcg
     2041 caatttgctg gcagagctcc gtgcacagca gattccggta gggctggctt ccgtctccct
     2101 gaatgcgccg acgattttag cggcgctgga gctgcgcgag tttttcacct tctgcgcgga
     2161 tgcttcccaa cttaaaaact cgaaaccgga tccggaaatc tttctcgctg cctgtgccgg
     2221 gctgggcgtg ccgccgcagg catgtatcgg cattgaagat gcgcaggcgg gcattgacgc
     2281 cattaacgcc agcggtatgc gctcggtggg gatcggtgcg ggcttaaccg gggcgcaatt
     2341 actgttgccc tcaacggact ccctgacctg gtcgcggtta tcggccttct ggcaaaacgt
     2401 atagcaaagg aatcaacatg gctcagcttt cgttacaaca tattcaaaaa atctacgata
     2461 accaggtgca tgtggtgaag gacttcaacc tggaaattgc cgataaagag ttcatcgtat
     2521 ttgtcggccc gtcgggctgc ggtaaatcga ccaccctgcg catgattgcc gggcttgagg
     2581 agatcagcgg cggcgatctg ttgatcgacg gcaaacgaat gaatgacgtt ccagccaaag
     2641 cacgcaatat cgcgatggtg ttccagaact acgcgttgta tccgcatatg acggtttacg
     2701 acaacatggc gtttggcctg aagatgcaaa aaatcgccaa agaggtgatt gatgagcggg
     2761 tgaactgggc ggcgcaaatt ctcggcctgc gtgagtacct gaaacgcaag ccgggcgcgc
     2821 tttccggcgg gcaacgccag cgtgtggcgc tcgggcgggc gattgtgcgc gaagcgggcg
     2881 tgtttttaat ggatgaaccg ctctctaacc ttgatgccaa gctgcgcgta caaatgcgtg
     2941 cagagatcag caagctgcat cagaaactga acaccaccat gatctacgtg acccacgatc
     3001 agaccgaagc gatgaccatg gcgacgcgga ttgtgattat gaaagacggg attgttcagc
     3061 aagtaggtgc gccgaaaacc gtttataacc aacccgcgaa tatgtttgtt gccggattta
     3121 ttggatcacc agcaatgaat tttattcgcg gcacgatcga tggcgataaa ttcgttacgg
     3181 aaacgcttaa attaaccatt ctcgaagaga aattagcggt tctgaaaaca caggaaagtt
     3241 tgcataagcc catcgtgatg ggaatacgcc cggaagatat tcatccggac gcgcaagagg
     3301 aaaataacat ttccgccaaa attagcgtgg cagaattaac cggtgcggaa tttatgctct
     3361 acaccacggt tggggggcac gagttagtgg tccgtgctgg tgcgttaaat gattatcatg
     3421 caggagaaaa tatcactatt cattttgata tgacgaaatg tcatttcttt gatgcagaaa
     3481 cggaaatagc aattcgctaa atacaggggg aaggcattcc cctaggataa tacaaggaac
     3541 aataatgaaa aagttattac cctgtaccgc actggtgatg tgtgcgggaa tggcctgcgc
     3601 acaggccgag gaaaagaacg actggcactt taatatcggt gcgatgtacg aaatagaaaa
     3661 cgtcgagggt tatggcgaag atatggatgg gttggcggag ccttcagtct attttaatgc
     3721 cgccaacggg ccgtggagaa ttgctctggc ctattatcag gaagggccgg tagattatag
     3781 cgcgggtaaa cgtggaacgt ggtttgatcg cccggagctg gaggtgcatt atcagttcct
     3841 cgaaagcgat gatttcagtt tcggcctgac cggcggtttc cgtaattatg gttatcacta
     3901 cgttgatgaa ccgggaaaag acacggcgaa tatgcagcgc tggaaaatcg cgccagactg
     3961 ggatgtgaaa ctgactgatg atttacgttt caacggttgg ttgtcgatgt ataaatttgc
     4021 caacgatttg aacacgaccg gttacgctga tacccgtgtc gaaacggaaa caggtctgca
     4081 atataccttc aacgaaacgg ttgctttgcg agtgaactat tatctcgagc gcggcttcaa
     4141 tatggacgac agccgcaata acggtgagtt ttccacacaa gaaattcgcg cctatttgcc
     4201 gctgacgctc ggcaaccact cggtgacgcc gtatacgcgc attgggctgg atcgctggag
     4261 taactgggac tggcaggatg atattgaacg tgaaggccat gattttaacc gtgtgggttt
     4321 attttacggt tatgatttcc agaacggact ttccgtttcg ctggaatacg cgtttgagtg
     4381 gcaggatcac gacgaaggcg acagcgataa attccattat gcaggtgtcg gcgtaaatta
     4441 ctcgttctga taatgggcta aattgccgga tgcggcgcga gtactttatc cgatctataa
     4501 atgtaggccg gataagatgc gccagcatcg catccggcat tcaggcaagg tagctggtat
     4561 ttatttcagc gtcatatgcg tggcaacggt aatattctgt ggtgacggtt ttcccgaaat
     4621 taagcggaat aataactcgc agctttgttg acctaactcc tgcgtcggaa catcgatgcc
     4681 gcctggtgca ggcgttaaaa taaacgacag cgtttcatta ctataaccca ccaccgctaa
     4741 ctgctgcgga atagcaatat ttttctctgc cgccgcacga taaatgctca ttaatttcag
     4801 gctgtcagtg gcaaacactg cctcaggcaa cggtgactga cttaataatt gccgtgctgc
     4861 ttgcagtgct gtttcatggg tataaccgcc gtcaacaatc catttatcac gcactgcaat
     4921 attatgcgcg gacagacttt tcttatagcc attaacgcga tcaaccgaaa catggacatc
     4981 aagcggagca tgcaggcagg caatattttg atgcccactt tcaattaatg catcggtcaa
     5041 cgcaatgctg tcaccaaaat tatcggtatc gacagaataa acatgggcat attgaccttc
     5101 aactttgcca attaccacca ccggaatatc gtatttatcg agttgggcaa aaaatgactc
     5161 atccgctggc gaactgagca taataatgcc tttaatcatt ttctgtttaa ttttgctttc
     5221 gcatttttgc agatcttctg ccggattgtg cgacgtttgt aatatcacgt cgaaaccttc
     5281 ttcttccgct ttggcggtga tggcatgcaa aacttcagag aaaaacggat tacccgccgt
     5341 agttttggtc gaacgggtag aaatcaccat aatggcatca aaacccgaag aggtcagcgc
     5401 gcgggccagc ttgtttggct gatactgtaa ttcttcaatg gcccgtaaca ctttttcgcg
     5461 cgcttccggg gagatattag tttgcttatt cagcacgcgt gatacggtgg attttgatac
     5521 gcctgcaacc ctggcaatat cataaatagt aggggacata ggtcaggaac tccgtccgaa
     5581 cgtgaatgct gcacatctta tggagttcgc ccgtcgatga caacacgggc agttgataat
     5641 caatggcttg gcccccacat tcatatcctt acgaatgatt tttttctggt cttcagagca
     5701 gcacaggaca gcaatgaagc gacttaaaaa tgaactcaat gcgctggtga atcggggtgt
     5761 cgacagacat ctgcgcctcg ctgtaaccgg acttagccgc agcggcaaaa cagcgtttat
     5821 caccgcgatg gttaatcagt tgctcaatat tcatgccgga gcacgtttgc cgctgttaag
     5881 cgcggtgcgt gaagagcgct tgctgggcgt aaaacgcatt ccccagcgtg actttggcat
     5941 tccgcgcttc acctatgatg aaggactggc gcagttatat ggtgatcctc ccgcctggcc
     6001 gacgcctacg cgcggcgtca gtgaaattcg cctggcgtta cgttttaaat cgaacgattc
     6061 gctgctgcgc cactttaagg atacctccac gctgtatctg gaaattgtgg attatcccgg
     6121 cgaatggttg ctcgacctgc cgatgctggc gcaggattac ttaagctggt cgcgccagat
     6181 gacgggctta ctcaatggtc agcgcggcga atggtcggcg aaatggcgaa tgatgtgcga
     6241 aggacttgac ccgctagcgc ctgccgacga aaaccggctg gcagacattg ccgccgtgtg
     6301 gaccgattat ctccaccact gtaaacagca ggggctgcac tttattcagc ctgggcgctt
     6361 tgtcttgccg ggagatatgg caggtgcgcc cgcgctgcaa ttcttcccgt ggccggatgt
     6421 cgatgcctgg ggcgagtcca aactggcgca ggccgataag cacaccaatg ccggaatgct
     6481 gcgcgagcgg tttaattatt actgcgagaa agtggtgaag gggttctata agaatcattt
     6541 tctgcgcttt gaccgccaga ttgtgctggt ggattgcctg caacctctca acagtgggcc
     6601 acaggcattt aatgatatgc gtctggcact gacgcagctg atgcaaagtt tccactacgg
     6661 gcagcgcacg ctgtttcgcc gcttattctc gccggttatc gacaaactgc tgtttgccgc
     6721 gacgaaagcg gaccatgtaa cggtcgatca gcacgccaat atggtttcat tactgcaaca
     6781 actgattcag gatgcctggc aaaatgcggc gtttgaaagg attagtatgg attgcctggg
     6841 gctggcctcg gtgcagacga ccaccagcgg cattattgac gtcaacggcg agaaaattcc
     6901 ggcattgcgc ggtaaccggc tcagcgatgg tgcgcctctc accgtttacc ccggtgaagt
     6961 tcctgcgcgt ttgcccggtc aggcgttctg ggacaagcaa gggttccagt ttgaagcgtt
     7021 tcgcccgcag gtgatggatg tcgacaaacc gctgccgcat attcgtcttg atgccgcgct
     7081 ggaattttta ataggagata aattgcgatg accgaaccgt taaaaccacg tattgatttc
     7141 gacggtccgc tggaggtcga acagaatcca aaattcaggg cgcagcagac ctttgacgaa
     7201 aatcaggcgc aaaattttgc cccggccacg ctcgacgaag cgcaggaaga agaggggcaa
     7261 gtcgaagcgg taatggacgc agcgttacgt ccgaaacgca gcctgtggcg caaaatggtg
     7321 atgggcgggc tggctctgtt tggcgcaagc gttgtcgggc agggtataca gtggacaatg
     7381 aatgcctggc aaacccagga ctgggtagcg ctgggtggat gtgccgctgg ggcattgatt
     7441 atcggcgctg gcgtaggttc tgtggtaaca gagtggcggc gcttatggcg cttgcgacag
     7501 cgcgcccatg aacgcgacga agcgcgtgat ttattgcata gccacggcac gggcaaaggt
     7561 cgcgcatttt gcgaaaaact ggcgcagcag gcgggtattg atcagtcgca tccggcgctg
     7621 caacgctggt atgcctcaat ccatgaaacg cagaacgatc gtgaagtggt cagcctgtat
     7681 gcgcatttgg tccagccagt tttagatgcc caggcgcggc gcgaaatcag ccgttcggcg
     7741 gcggaatcaa cgttgatgat tgcggtcagc ccgctggcgt tggtcgatat ggcgtttatc
     7801 gcctggcgca atctgcgttt aattaatcgc attgccacgc tgtatggcat tgaactgggg
     7861 tattacagcc gtttgcgtct gtttaagctg gtattgctga atatcgcttt tgccggagcc
     7921 agcgaactgg tgcgcgaagt ggggatggac tggatgtcgc aagatctcgc tgctcgtttg
     7981 tctacccgcg cagctcaggg aattggtgct ggacttctga cggcacgact cgggattaaa
     8041 gctatggagc tttgccgccc gctgccgtgg attgacgatg acaaacctcg ccttggggat
     8101 ttccgtcgtc agcttatcgg tcaggtgaaa gaaactctgc aaaaaggcaa aacgcccagc
     8161 gaaaaataat gcaatatcgg gtgctgaccg gatatcttta cgccgaagtg cccgtttttc
     8221 cgtctttgtg tcaatgattg ttgacagaaa ccttcctgct atccaaatag tgtcatatca
     8281 tcatattaat tgttcttttt tcaggtgaag gttcccatgc gtctggaagt cttttgtgaa
     8341 gaccgactcg gtctgacccg cgaattactc gatctactcg tgctaagagg cattgattta
     8401 cgcggtattg agattgatcc cattgggcga atctacctca attttgctga actggagttt
     8461 gagagtttca gcagtttgat ggccgaaata cgccgtattg caggtgttac cgatgtgcgt
     8521 actgtcccgt ggatgccttc cgaacgtgag catctggcgt tgagcgcgtt actggaggcg
     8581 ttgcctgaac ctgtgctctc tgtcgatatg aaaagcaaag tggatatggc gaacccggcg
     8641 agctgtcagc tttttgggca aaaattggat cgcctgcgca accataccgc cgcacaattg
     8701 attaacggct ttaatttttt acgttggctg gaaagcgaac cgcaagattc gcataacgag
     8761 catgtcgtta ttaatgggca gaatttcctg atggagatta cgcctgttta tcttcaggat
     8821 gaaaatgatc aacacgtcct gaccggtgcg gtggtgatgt tgcgatcaac gattcgtatg
     8881 ggccgccagt tgcaaaatgt cgccgcccag gacgtcagcg ccttcagcca aattgtcgcc
     8941 gtcagcccga aaatgaagca cgttgtcgaa caggcgcaga aactggcgat gctaagcgcg
     9001 ccgctgctga ttacgggtga cacaggtaca ggtaaagatc tttttgccta cgcctgccat
     9061 caggcaagcc ccagagcggg caaaccttac ctggcgctga attgcgcgtc tataccagaa
     9121 gatgcggtcg agagtgaact gtttggtcat gctccggaag ggaagaaagg gttctttgag
     9181 caggcgaacg gtggttcggt gctgttggat gaaatagggg aaatgtcacc acggatgcag
     9241 gcgaaattac tgcgttttct taatgatggc actttccgtc gggttggcga agaccatgag
     9301 gtgcatgtcg atgtgcgggt gatttgcgct acgcagaaga atctggtcga actggtgcaa
     9361 aaaggcgtgt tccgtgaaga tctctattat cgtctgaacg tgttgacgct caatctgccg
     9421 ccgctacgtg actgtccgca ggacattatg ccgttaactg agctgttcgt cgcccgcttt
     9481 gccgacgagc agggcgtgcc gcgtccgaaa ctggccgctg acctgaatac tgtacttaca
     9541 cgttatgcgt ggccgggaaa tgtgcggcag ttaaagaacg ctatctatcg cgcactgaca
     9601 caactggacg gttatgagct gcgtccacag gatattttgt tgccggatta tgacgcagca
     9661 acggtagccg tgggcgaaga tgcgatggaa ggttcgctgg acgaaatcac cagccgtttt
     9721 gaacgctcgg tattaaccca gctttatcgc aattatccca gcacgcgcaa actggcaaaa
     9781 cgtctcggcg tttcacatac cgcgattgcc aataagttgc gggaatatgg tctgagtcag
     9841 aagaagaacg aagagtaagc gcgaatatgc ctgatggtgc aacaccatca ggcatattaa
     9901 cttatgcttt cagtacagcc agagctgctt cgtaatccgg ctcggtggtg atttcatcca
     9961 ccagctggct gaaaatcaca ttgtcatttt cgtcaataac cacaacggca cgcgctgcca
    10021 gacctttcag taggccatca gcaattgcca caccgtaggc ttgcagaaat tcagcgttac
    10081 ggaaagtgga gagggtgata acgttgttca gaccttctgc gccgcagaaa cgagactggg
    10141 cgaacggcag atcggcagag atacacagca caacggtgtt gtcgatctca gttgccagtt
    10201 ggttaaactt acgtactgat gcggcgcaaa caccggtatc aatactcggg aaaatgttca
    10261 gcactttgcg tttacccgca aactgaccga gggtgacgtc agacagatct tttgccacga
    10321 gagtaaaagt ctgcgctttg ctacccgcct gcgggatgga attggcgact gtaaccgggt
    10381 tgccctggaa atgaacggtt tgtgacatga ttatctttcc tgtttacata tagttaacgt
    10441 cacacctagt ttatgctaac tgtcaataac acagcaaacg ctatttgcgc ttaatccgca
    10501 gaccaccgcg acaacaagga gtaaagatga gaatcgttaa ggtatttgag gaagcctggc
    10561 ccttacatac cccgtttgtg attgcccggg gaagccgcag tgaagcgcgc gtggtggtgg
    10621 ttgaactgga agaagagggt attaaaggca ccggcgaatg cacgccgtat ccgcgttatg
    10681 gagaaagtga tgcctcggta atggcgcaaa ttatgagcgt cgtgccgcaa ttagagaaag
    10741 ggctgacacg ggaggagttg caaaaaattc tccctgccgg cgcagcacgt aatgcgctgg
    10801 attgtgcatt gtgggatctc gcggcgcgaa aacagcagca atcgctggct gatttgatcg
    10861 gcataacgct tcccgagaca gttatcactg cacagacggt tgtcatcggt acgcctgatc
    10921 agatggccaa tagtgcatca acactctggc aggcaggcgc gaaattactg aaagtgaagc
    10981 tggataacca tcttatcagt gagcggatgg tggcaattcg caccgctgtg cccgatgcca
    11041 cgctgatcgt tgatgcaaat gaatcctggc gtgcagaagg gttggcggcg cgttgccagc
    11101 tattggcgga tttaggcgtt gcgatgcttg aacaaccgct tcctgcgcag gacgatgcgg
    11161 cgctggagaa ttttattcat ccgttgccga tttgtgctga tgaaagttgt catactcgta
    11221 gcaatctgaa ggcgctgaaa gggcgctatg agatggttaa cattaagctc gataaaaccg
    11281 gtggtctgac ggaagcgctg gcgactgaag cgcgtgcaca aggtttcagt ctgatgctgg
    11341 gctgcatgtt gtgtacctct cgtgccatta gcgccgcttt accgctggtg ccgcaggtca
    11401 gtttcgccga tcttgacgga ccgacctggc tggcggtaga tgtggaaccg gcgcttcagt
    11461 tcacgacggg cgaattgcat ctttaggatg ccagcgcagc aagtttgcca tcgcaaagag
    11521 atatttttcg ctggcttcgt cggaggagat gggcggaaac tctgcggtga tgcaatgcaa
    11581 gttgagatct gcacaccaac tgccgaaaga gccaggcgtt tcataaccga cgctggttac
    11641 cagaggcaat tcaaacgact gggccagcca ttcgcctaat tcgctgtgtc tgggatcttc
    11701 aatacaagcc agtggatcgt ggaatgagac cacccaggca ggttgaatgc ggtgtataag
    11761 ctggcacagc gcctgggttt caggttcgga gccaggtttg tcacctgtca gtaatacgac
    11821 atcacgttct tcagcggcgc tgttccagcg ataaaccgtt tcaccttcct tccagttcgc
    11881 cgccggaaag tttctgttta aatccacacc attagcattg gcccgtaacc ccagctggca
    11941 gccgtcagga ttcacacaca gcaccacatg atggcggcgc aaagaaggtg tcaaagtccg
    12001 cagagcacag gagagcgtga cgaccgaaga gttttcatca ccgtgagtgc cagccagaat
    12061 caaaccactt tcgcggctgg ctgcaggggc aggaaaccag attaacggcg cgcccaataa
    12121 tgaacgtccg taatgttctg ttccgggcgg aaatgcgccg cgttcggcgc gtgggcgggt
    12181 tacggtcatg atcgtctctg gttcaggaag tattacaggc agtgttgtgc aaattttgcg
    12241 gattatcaaa tccttttctt caacgctatg tcatcgatct tgtatcactt ttttgccgga
    12301 agttgtttgc attttcattg atgtgcagat attagaattg tcgtaatgtt atatacatgg
    12361 cgtattttat tcttactggt tctacacatg ttaatcgcat ctgattgatc agttcacctt
    12421 aataagttta ataaatcgta ctgcacatag tacagtacga tggtatctat ataattgatt
    12481 tacatgaaga atggcttatc gccatcagtc ccgggttgat tgatgcctat attctcacca
    12541 atgtgtttct caggtttatc gatgatatct gtaattaatg ctgcgacact cttacgtgat
    12601 acgatcgtac ctttgaatgg ttcattgcga gaggtaagct cataatcaat aatatcttca
    12661 tcagtcagct aggcagggcg aagaattgta tattccagtc cagaagcctc aattgcatcc
    12721 gcagccctgc ggaatggttt taggggttca ccaataactg cattattcca ttcgacaaat
    12781 tttcctggaa cttcatcata aataccgagg gaaagaacaa agatcagacg tttaacatca
    12841 caggctttca tcgcagcgat cactgaattt gcctgaatat ctaaatcttc ccccgtaagg
    12901 ttggcataca caatatcctg tccctgcatg gcctgcttca gtgctgcatg attcagtaca
    12961 tcccccataa taatctggct attagttgga tagggtttgt gtatttttgc tggctgacgg
    13021 gcaaataaag tttgtttaat agtttgttta tctgccagtt gattaatcac atggcgggct
    13081 atctgaccgc cagcaccaag aatcagtaca tttttcataa tatttctcat gaaacttatc
    13141 ccagatgggt gttgaaaaaa ggaataactt ttgccagcgc ctcctgaact gcttccggtt
    13201 tgtcatatag ttcataatgt gagaaatcgg gtaacgaaac taactgacga tcctgcgaaa
    13261 ccatcgcccg gccatagact tccagtccat cacgataagc accgaaggca ccgacttttt
    13321 caccaactac taccatcacc ggctgggtca gtaacacttc agtaaatgcg aaggcatccc
    13381 aggccagcgt tttctgtgca tgtgagaaca acatttttgc cgcacctccc ggttgttgcc
    13441 cgcgtggtgt tttatagtag tcagttgctt cataaacatc gcgttcggtc agtccatggg
    13501 cttttgctgc atccaggctg gcgggaagta attcgttgat ctgtaattct ccaccacgag
    13561 cctcttttgt tcgttgtgac gccattgcat tcaatacgcc aatcggatca taattgctga
    13621 aaccttcccg aaaaagacgc ccgatattaa cacccgtaat actcactaca gctttaattc
    13681 gtttttctgt cagcgcggca ttaatggcat agccacccgt tactttattc gtacccctta
    13741 taatggggtg ttagccagcc agacccggca tgattactgc ccccagtcgt ccatgatccg
    13801 gggggtgatg tcacagggtc tggtgggg
//
LOCUS       DAETPZ010000097        13826 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.097, whole genome shotgun sequence.
ACCESSION   DAETPZ010000097 DAETPZ010000000
VERSION     DAETPZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.097"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            166..1419
                     /gene="kch"
                     /locus_tag="K0W59_003291"
     CDS             166..1419
                     /gene="kch"
                     /locus_tag="K0W59_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HBI3024211.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1474..1647)
                     /locus_tag="K0W59_003292"
     CDS             complement(1474..1647)
                     /locus_tag="K0W59_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HBI3024212.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1790..3250
                     /gene="cls"
                     /locus_tag="K0W59_003293"
     CDS             1790..3250
                     /gene="cls"
                     /locus_tag="K0W59_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBI3024213.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3285..3614
                     /locus_tag="K0W59_003294"
     CDS             3285..3614
                     /locus_tag="K0W59_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HBI3024214.1"
                     /translation="MDMDLNNRLTEDETLKQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPVEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3667..4671)
                     /gene="oppF"
                     /locus_tag="K0W59_003295"
     CDS             complement(3667..4671)
                     /gene="oppF"
                     /locus_tag="K0W59_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HBI3024215.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4668..5681)
                     /gene="oppD"
                     /locus_tag="K0W59_003296"
     CDS             complement(4668..5681)
                     /gene="oppD"
                     /locus_tag="K0W59_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HBI3024216.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTLGRLRACFKPVE
                     ELL"
     gene            complement(5693..6601)
                     /gene="oppC"
                     /locus_tag="K0W59_003297"
     CDS             complement(5693..6601)
                     /gene="oppC"
                     /locus_tag="K0W59_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HBI3024217.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6616..7536)
                     /gene="oppB"
                     /locus_tag="K0W59_003298"
     CDS             complement(6616..7536)
                     /gene="oppB"
                     /locus_tag="K0W59_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HBI3024218.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7622..9253)
                     /gene="oppA"
                     /locus_tag="K0W59_003299"
     CDS             complement(7622..9253)
                     /gene="oppA"
                     /locus_tag="K0W59_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HBI3024219.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(9991..10638)
                     /gene="ychE"
                     /locus_tag="K0W59_003300"
     CDS             complement(9991..10638)
                     /gene="ychE"
                     /locus_tag="K0W59_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HBI3024220.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11115..13790
                     /gene="adhE"
                     /locus_tag="K0W59_003301"
                     /gene_synonym="adhC"
     CDS             11115..13790
                     /gene="adhE"
                     /locus_tag="K0W59_003301"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBI3024221.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3627 a         3426 c         3221 g         3552 t
ORIGIN      
        1 gttcaggaaa aaagcccttt cctgacgcag agttgctcac tcaaaacctt tgattactgc
       61 ggataaagtc atctactatt gtttaacaca ttcacgcgct gcatgtatgt tcagaaaatg
      121 tgcacgctaa gataattaaa caaaattgtg gttacagagg tatcagtgag tcactgggct
      181 acattcaaac aaacagcaac aaatttatgg gtgaccctac ggcacgacat tctcgcgctg
      241 gccgtctttt taaatggatt gcttattttt aaaacaatct atggtatgtc ggtcaatttg
      301 cttgatattt tccatatcaa agcattttca gagttggatc tctccttgct ggcaaacgcc
      361 ccactattta tgctcggcgt ctttcttgtc ctgaactcca ttggcttact gttccgggca
      421 aagctcgcat gggcaatcag tatcattttg ttgttgatag cgctaattta caccctgcat
      481 ttttatccct ggctgaaatt tagtattgga ttttgcattt ttacgctggt gtttttgctg
      541 atactgcgca aagacttctc ccacagtagc gccgcagccg ggacaatttt tgcatttatt
      601 agtttcacga cattactgtt ttactcaacc tacggtgcac tttatttaag cgaaggtttt
      661 aatccgcgaa tagaaagttt gatgaccgcg ttctattttt cgatagaaac catgtcaacc
      721 gtcggctatg gagatattgt ccctgtttct gaatcagcac gattgttcac tatttcggtc
      781 attatttccg gcattaccgt ttttgccaca tctatgacct caatttttgg tccgcttatc
      841 cgcgggggat tcaacaaact tttaaaagga aacaatcata caatgcatcg taaagatcat
      901 tttattgttt gcggacattc gattctcgcc atcaatacga tcctgcaact gaatcaacgc
      961 ggacaaaacg taacggttat cagcaacttg cctgaagatg atatcaagca acttgagcaa
     1021 cgcttaggcg ataacgctga tgttatcccc ggtgacagta atgacagttc agtattaaag
     1081 aaagcgggaa tcgatcgttg ccgggccatt ctggcgctga gtgataacga tgcagataac
     1141 gcgtttgttg tactctcggc aaaagatatg agcagtgatg tcaaaacagt tctcgccgtc
     1201 agtgatagca aaaacctgaa taagattaag atggtacatc cggatatcat tctctcaccg
     1261 caactgtttg gcagcgaaat tctggcgcga gttttaaatg gtgaagagat taataatgat
     1321 atgctcgttt caatgttgtt gaactcaggt catggtattt tcagcgataa cgatgaacaa
     1381 gaaacgaaag ctgacagtaa agaatcggcg caaaaataga gtttaacgac ggaagagtaa
     1441 acttattggg caccgcaatg gtgcccagta catttagata ttgtagatcg caaacaataa
     1501 cgagttacgc tgtttgttta gaatgcactt cctgatgctg tggatacgca tatttcggcg
     1561 cgattgcccc tcaagccaac gcgatttacg tcggctagcc tgacgctgca tgcgccagcg
     1621 ccccacttcc gttctactcc gcttcatgtt aactactcta tgcaataaca gaatggtcat
     1681 tatacccccg cccccgacgc tgaccagtgc ttttcccgcg tttttatttg ccagatgaat
     1741 ccatatgcgt aaactcataa caatgcgctt tcaaaaggat ttctaatcta tgacaaccgt
     1801 ttatacgttg gtgagttggt tggccattct gggatactgg ttgctcattg caggcgtaac
     1861 tttacgcatt ctaatgaaac gacgcgcagt tccctccgcg atggcctggc tgttgattat
     1921 ttacattctg ccgttagtcg gaattattgc ctatcttgcc gttggcgagc tccatttagg
     1981 caaacgccgc gctgagcgcg ccagagcgat gtggccttcc accgcaaaat ggcttaacga
     2041 ccttaaagcc tgtaagcata tcttcgccga agaaaatagc agtgtcgctg cgccattatt
     2101 caagctttgc gagcgtcgtc aggggatcgc tggggtcaaa gggaatcagc tacaactgat
     2161 gaccgagtca gatgatgtga tgcaggcgtt aatccgcgac atccagctcg cgcgccataa
     2221 tattgagatg gtgttttata tctggcagcc cggcggcatg gcggaccagg tggctgaatc
     2281 attaatggcg gctgcacggc gtggtattca ttgccgattg atgctcgact ccgccgggag
     2341 tgtggctttt ttccgcagcc cgtggcccga gctaatgcgt aatgccggta ttgaagtggt
     2401 cgaagcctta aaggtcaatc tgatgcgtgt gtttttacgc cgtatggacc tgcgccaaca
     2461 tcgcaagatg atcatgatcg ataattacat cgcgtacacc ggcagcatga atatggtcga
     2521 tcctcgctac ttcaaacaag atgcgggcgt agggcaatgg attgatctga tggcgcgtat
     2581 ggaaggcccc atcgccaccg cgatggggat tatttattcc tgcgactggg agattgaaac
     2641 cggaaaacgt attctgccgc caccaccaga tgtcaatatt atgccgtttg aacaggccag
     2701 tggtcacacc attcacacaa ttgcttctgg ccccggcttt ccggaagatc tcattcacca
     2761 ggcattattg actgcggctt attcggcgca tgaatatttg atcatgacca cgccctactt
     2821 tgtgccaagc gatgatttac ttcatgcgat ttgcacggcg gcgcagcgcg gggtggatgt
     2881 cagtattatc cttccgcgaa aaaatgactc gatgctggtc ggctgggcca gtcgcgcatt
     2941 ctttacggaa ctgcttgctg ctggggttaa aatttatcag tttgaaggcg ggttactgca
     3001 taccaagagc gtgctggtcg atggcgaact aagtctggtt ggcaccgtta accttgatat
     3061 gcgtagtctg tggctgaatt tcgagattac cctggcaatc gacgataaag gttttggtgc
     3121 agaccttgcc gccgttcagg acgattatat ttcgcgttca cgtctgctcg atgcccgttt
     3181 atggctaaaa cgtccattat ggcaacgtgt cgccgagcga ctgttttact tcttcagccc
     3241 gttgctgtaa aacgtcgcca acagaggtta aacaggtagt gactatggat atggatctaa
     3301 acaatcgcct gactgaagat gaaacgctta agcaggccta cgatattttt ctcgaactgg
     3361 ctgccgacaa tctcgatcca gctgatgttc tgctgttcaa tcttcagttt gaagagcgcg
     3421 gcggcgcgga gttattcgat ccggtggaag actggcagga acatgttgat ttcgatttga
     3481 acccggactt ttttgccgaa gtggtgattg gtctggcgga cagtgaagat ggtgaaatca
     3541 acgatgtttt cgcccgcatt ctgttatgcc gcgaaaaaga tcacaaactt tgccatatca
     3601 tctggcggga ataaataatc gcttttagcg tgaaaaaggg ctgacaactg tcagccctta
     3661 ttgtttttaa agcggatcga ctttcaggca agaaacggcg tgtctgaaac tcccctccag
     3721 aacaggacgt gttttggcgc actccggacc ggcaatcggg caacgggtac ggaaaacaca
     3781 accggaaggc gggttgatcg gcgacggtaa ttccccttcc agtaactgga tggttttgtt
     3841 cttctccaga tccggatcag gtatggggac tgccgacatc aatgccctgg tgtaaggatg
     3901 tagtggattg tggtagacct catcataggt ccccagttct actgcatggc cgagatacat
     3961 caccaacaca cgatcggaaa tgtgttttac cacggccagg tcatgagcaa taaaaattaa
     4021 tgacaatccc atctcacgtt gcagctgctg gagcaggttg accacctgcg cctgaattga
     4081 cacgtccagc gccgaaaccg gctcatcgca gataatcagc ttcggttcaa gaataagagc
     4141 acgagcaatc ccgatacgct ggcactgccc accggagaac tcatgcggat agcggttaat
     4201 caggttaggc aataacccga ctttcagcat catcgccttc acgcgctcgc gaacttcctg
     4261 gcgtgacatt ttcggatgat aagtacgcag tggttcagcg atgatctcgc cgatggtcat
     4321 acgcgggttt agcgatgcca acggatcctg gaaaatcatc tgaatatcac tgcgaacggc
     4381 acgccattca tcgggcttca tgcccagcaa ctctttacct aaccaggcaa catgaccgtc
     4441 ggtcgccttg accaaaccga tgatggcgcg agcaaaggtg gacttaccgc atcccgattc
     4501 ccctaccaca cctaatgttt ccccttcata caggcgaaga gtgacaccat cgacggcttt
     4561 gagcgttttc ggcggttgcc agaaccactg tttgccatct ttgatttcaa agtgcacttt
     4621 aagatcggcg atttcaagga ggacttttct tccttcagtt acagcattca taacagttcc
     4681 tccaccggtt taaagcaagc acgcagacgg ccaagcgtaa actcttccag cggcggtgcg
     4741 ctactacaaa tttccatcgc atgtggacaa cgtggctgga acgggcaacc tttcggtaat
     4801 cgcagcaagt ttggcggatt acccgggatg gtcaacattg tttcaccttc cgcatcaaga
     4861 cgcggcaccg cgttgagcaa accgatagaa taaggatgaa cgggttgata aaagacatcg
     4921 cgcgcattgc catattccat cgtgcgcccg gcgtacatta ccagcacttt gtcacagatc
     4981 cccgccacca cgccaagatc gtgggtgatc ataatgatgg cagtattaaa ttcccgcttc
     5041 agttcattca atagcgtcat gatctgcgcc tgtacggtga cgtccagcgc tgtagtgggt
     5101 tcatccgcaa tcagcaactt aggtcgacat agcaatgcca tcgcaatcat gactcgctga
     5161 cgcatgccgc cagaaaattc gtgcgggtac attttcatgc gtttacgtgc ttccggcatt
     5221 tttaccgcat cgagcatccg caccgactct tcaaacgcct cagctttgct catgttctta
     5281 tgcagcatca gcacttccat caactgctca ccgacgcgca tatagggatt caacgaagtc
     5341 attgggtcct gaaaaatcat tgagatttgt tcagcgcgca gtttattgag ttcacgctct
     5401 ggcaaattga ggatttcacg cccattgaag gttgccgatc cgccaatgcg tccgttggca
     5461 gccaacaggc ccatcaacgc aaatgcagtt tgcgatttac ccgaaccaga ctcacctaca
     5521 atgcccagcg tttctccggc acgtagggaa aaatttaaat cattgaccgc cgtgacgtcg
     5581 ccgtccgggg tactaaaggt gacacgcaaa tctttcacgt tcagcagtgc gtcagcctgt
     5641 tgttgtgcga gcggcacagt tgcagtttca attacgctca tggctgcact ccttaacgat
     5701 ctttcgggtc gagggcatca cgcaagccat cgccgataaa gttgaaacaa aacagtgtca
     5761 ccacaaggaa tcccgctggg aacaacagta accatggaga gacttccatc gagttcgcgc
     5821 catcactcag caatgccccc cagctgctta acggctcttg cgtacccaac cccaggaagc
     5881 taaggaaaga ttcaaagagg atcatgctgg gcaccagtag cgatgcgtag accaccacca
     5941 caccgagtac gttcggcaca atgtggcgaa taacaatgcc cggcgtcgat acaccgccaa
     6001 cttgtgccgc ctcaataaac tctttgcgct tcagactcag ggtttgccca cgcacaatac
     6061 gagccatatc cagccaggaa accatgccaa tcgccacgaa aatcagcagg atgttttggc
     6121 cgaaaaaggt caccagcaaa atgacgaaga acatgaatgg gaaggagttg aggatttcca
     6181 gcagacgcat cattaccgaa tccactttac cgcccagata accggaaagc gaaccataaa
     6241 gtgtccccac gaccactgcc accagtgccg cagcaacacc gaccatgagt gagatacgcc
     6301 cgccaatcgc aacgcgcaca agcaggtcgc gaccggatga gtcagtacca aagtagtgac
     6361 cggactccat atccggggcg ctggacatca tcgcccagtc agtatcgtca taggcaaact
     6421 gggaaagcat cggtgccagg attacaaata acgcgatcag caccagcact atcagactgg
     6481 caaccgccgc acggttatgc ataaaacgtc gacgtgcgtc ctgccacaag ctgcgccctt
     6541 cgacctccag cttttcactg aaattttcca gcgtctcgct gtttttctta cttaacatca
     6601 tcgcgagctc cagtatcagt aacggatttt cgggtcgata accgcatata gcacatcgac
     6661 aatggcatta aacaaaatgg ttaacgcacc aaccaggatg gtcaggctta acactaagga
     6721 atagtcacgg ttcaatgcgc cattaacgaa cagttgccca atccccggca aaccataaat
     6781 ggtttcgatg accatagaac cggtaataat gccgacaaat gcggggccca tataggagag
     6841 cacgggtaac agagcaggtt ttaatgcgtg gcgtaaaatg atccgccgca taggtaaccc
     6901 tttcgcccgg gcagtacgaa taaagttgga gtgtaatact tcaatcatag agccacgggt
     6961 aatacgcgca atactggcga tataggcgag tgacaacgcc accatcggca atatcatgaa
     7021 tttaagcgcc ccaccattcc agccaccgcc cggcagccaa tgcaaaatga tcgcaaatat
     7081 catgactaat aatggcgcaa ccacaaaact ggggataaca accccggtca ttgccagccc
     7141 catcacggta tagtcccatt tggtgttttg ttttaatgcg gcaataacgc cagcactaac
     7201 acccagtatt accgcaagga aaaatgcggc ggctcccagt ttggcagaaa cgggaaaact
     7261 ggatgccacc aggtcattga ccgaataatc tttatattta aacgatggac cgaaatcgcc
     7321 atgcgccagt tgtttcaggt agctgaaata ctgtgtcatg atcggatcat taagatgata
     7381 tttcgcttcg atattggcca tcacttctgg cggtaaagta cgttcgccgg taaaagggct
     7441 tcccggcgcg aggcgcatca taaagaacga aatagtaata agaataaata gcgtcggaat
     7501 cgcttccaga cagcgacgta gaataaattt taacattgcc ctaccttctg gcctgtgcct
     7561 tctgtaatgc gataaaaatc agacaccgtg gagcaggaca ctcctgcccc acgtattgcc
     7621 attagtgctt cacaatgtac atattccggg tataggtatt atccagcgga tctttgccgg
     7681 tatagccacc aacccacggt ttcaccagac gcgcattcac gtagtaataa acaggaacaa
     7741 tggccgaatc cttatccagc tgttgttctg ctttagtgta cagagctgtg cgctgcgcct
     7801 cgtcagtcac tttcagcgtt tccgccataa tgctgtcaaa ggccgggctc ttataatgcg
     7861 cggtattcat cgagctgttc gacagcatgg tgttcaggaa ggaagttggt tcgttgtagt
     7921 ctgcacacca gcctgcacgg gccacatcaa aagtgccctg gtgacgggtg tcgaggaacg
     7981 ttttccactc ctggttaacc agtttgacgt ttacaccaat gtttttcttc cacaatgaag
     8041 aggcagcaat cgccagcttt ttatgcagat cggaggtgtt ataccgcagg ttgatggtca
     8101 acggtttgtc tgcggtataa cccgcttcag ccagcagttt tttcgcttct tcgttacgtt
     8161 tttcctggct ccagccaaac cattctggct gagtcaattt tgcgccatca gtatacggtg
     8221 gagtgtaacc ataggcgggc atgtcgccct gcgctttcac tttattaaca atgatatcgc
     8281 ggtccatacc tagtttcagt gcggtacgca cacgcacatc gttgaatggc ggtttctggt
     8341 tgttaatttc gtaatagtaa gtgcacaggt atggatcaac gtgaacttcg tccgggatct
     8401 cttttttcag cttctggaac aattcgatcg gcatgttgtt atacgtcatg tcgatttcac
     8461 cactacggta gcggttgaca tcggtaactt cagaagcaat aggcaaatag gttacctggt
     8521 taataacggt tttcgcgttg ttccagtagg tcgggctgcg ttcaagaacg attcgttcgt
     8581 ttacgaccca atcttttaag gtataggcac cgttggtgac gatattacca ggctgggtcc
     8641 atttttcgcc gaatttctcg atagcggctt ttggcaccgg tgaagttgat gggtgaacaa
     8701 gtaatttata gaagtacgga acgggttcac ttaaggtgac ttctaatgtg tgatcatcaa
     8761 tagctttcac gccgagatcg gtaatcggtt ttttcccttc aagaatttca tcaataccgg
     8821 cgatatgccc atattgcaga taactggcat acggagaagc agtgttcgga tcaacagaac
     8881 gttgccagct atacacaaag tcttgtgctg tgactggcgt gccatcagac catttcgcat
     8941 ctttacgcaa atggaaggtc cagactttcg cgtctttatt atcccaggat tcagcgacgc
     9001 caggtgctgg atgaccgtca agatcgctga ccagtaagcc ttcaaacagg tctcggctga
     9061 tattagactc cggaacacct tcaattttgt gcggatctaa tgactgaact tctgaaccat
     9121 tgttacgtac cagtgtttgt ttttccgcca gtgtgacgcc tgcgggtaca tcagctgcca
     9181 gcgcgacatt ccctgccatt agcgcagcca gaacgccagc tgctactaaa cttctcttgg
     9241 tgatgttggt cattgttttt tggactccct cattataatt actggtatta accagcatgt
     9301 gtaatcccgg attgggaccc tgcacaggct cggagacttt ctgctgtcag gttactttat
     9361 cgttacgtat tactatcacc gactttattt attctggtga ttcagacgat caccttatgt
     9421 cgattcgtac tgtgtctccc ctgtcgagac acattattct ttcgcgacgc tctttaattg
     9481 ttaattattc tcattaccaa taaagaatcg tctggcggtt gcccggaaag taccaaatgc
     9541 ggttcttact cgccaataca ttttgcaaaa ttgttaagcg attctctttt attgaccgta
     9601 tcagaatcta tagcttgcat ccatgcctta acaaacatct ttcaattcaa tcaaatagag
     9661 attggctaag catgtctatc ttttctgatt acagttccag tagtgaaatg cacaataatt
     9721 taacaattga ttattattta gcactttcct ctaccaaagg tagtggaatt actaatatca
     9781 tttcaattat tcttcagcaa gcccaggatt atgatgtggc gaaaataaca tgatggtgag
     9841 gagaagcagt taaggaaatc cttataacta actgtaattt gagaagaata tttttaccta
     9901 ttccttttgc ttttgataac tgcgcgggaa caaagtaaaa ataatgacga agtaaacgga
     9961 gcatggcagc tccgtttcat ttgaaaggaa ttaattaagc aggccgggga aaatcccctt
    10021 aataccagta acgataaatt caatccccaa tgccatcagc aatagcccca taatacgcgt
    10081 aatcacgttg atgccggtct ggcgtaaaac ccgtaccagc cacggtgcca tgcggaacaa
    10141 tccccagcaa cataaagcga acaatgcaat agccacaaag aaaccaaaca gatagctaat
    10201 gctgtgataa cgcgtacccc agacgatggt agaactgatc gcccctggcc ccgccatcaa
    10261 cggcaacgcc aatggcacca caccaatact ttcacgtacc gcggtttctg atttttcttg
    10321 cttgttctgt ttatcctcgc caagcttgcc gctgatcatc gacatcgcta tcgtcaccac
    10381 caggatgccc ccggcgatac ggaacgaatc aattgatata ccaaaaagtt gtagaatcgt
    10441 gtcgccgaga aaaagcgaga tccacaagat aatggccaca gacaggttgg ctgtaaggtt
    10501 agttttgttt cgcgctgccg ctgtctgata actggtcatg ctgataaaga cgggaataat
    10561 ccctaccggg ttgaccagcg caaataaccc gatgaaaaat ttgaagtaaa cgggaaaatc
    10621 aaaaagggtc tgaatcacgg ttagctccga agcaaaagcc ggataatgtt agccataaat
    10681 aaggttgaaa agacgcgctg acaatacgcc ttttgacagc atttttcacc tcctaactac
    10741 ttaaaattgc tatcattcgt tattgttatc tagttgtgca aaacatgcta atgtagccac
    10801 caaatcatac tacaatttat taactgttag ctataatggc gaaaagcgat gctgaaaggt
    10861 gtcagctttg caaaaatttg atttggatca cgtaatcagt acccagaagt gagtaatctt
    10921 gcttacgcca cctggaagtg acgcattaga gataataact ctaatgttta aactctttta
    10981 gtaaatcaca gtgagtgtga gcgcgaataa gcttttgatt ttcattggtt aagcaaatca
    11041 tcaccgcact gactatactc tcgtattcga gcagatgatt tactaaaaaa gtttaacatt
    11101 atcaggagag cattatggct gttactaatg tcgctgaact taacgcactc gtagagcgtg
    11161 taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga gcaagtagac aaaatcttcc
    11221 gcgccgccgc tctggctgct gcagatgctc gaatcccact cgcgaaaatg gccgttgccg
    11281 aatccggcat gggtatcgtc gaagataaag tgatcaaaaa ccactttgct tctgaatata
    11341 tctacaacgc ctataaagat gaaaaaacct gtggtgttct gtctgaagac gacacttttg
    11401 gtaccatcac tatcgctgaa ccaatcggta ttatttgcgg tatcgttccg accactaacc
    11461 cgacttcaac tgctatcttc aaatcgctga tcagcctgaa gacccgtaac gccattatct
    11521 tctccccgca cccgcgtgca aaagatgcaa ccaacaaagc ggctgatatc gttctgcagg
    11581 ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg gatcgatcaa ccttctgttg
    11641 aactgtctaa cgcactgatg caccacccag acatcaacct gatcctcgcg actggtggtc
    11701 cgggcatggt taaagccgca tacagctccg gtaaaccagc tatcggtgta ggcgcgggta
    11761 acactccagt tgttattgat gaaactgctg atatcaaacg tgcagttgca tctgtactga
    11821 tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga acagtctgtt gttgttgttg
    11881 actctgttta tgacgcagta cgtgaacgtt tcgcaaccca cggcggctat ctgttgcagg
    11941 gtaaagagct gaaagctgtt caggatgtta tcctgaaaaa tggtgcgctg aacgcggcta
    12001 tcgttggtca gccagcctat aaaattgctg aactggcagg cttctctgta ccagaaaaca
    12061 ccaagattct gatcggtgaa gtgaccgttg ttgatgaaag cgaaccgttc gcacatgaaa
    12121 aactgtcccc gactctggca atgtaccgcg ctaaagattt cgaagacgcg gtagaaaaag
    12181 cagagaaact ggttgctatg ggcggtatcg gtcatacctc ttgcctgtac actgaccagg
    12241 ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat gaaaacagct cgtatcctga
    12301 ttaacacccc ggcttctcag ggtggtatcg gtgacctgta taacttcaaa ctcgcacctt
    12361 ccctgactct gggttgtggt tcctggggtg gtaactccat ctctgaaaac gttggtccga
    12421 aacacctgat caacaagaaa accgttgcta agcgagctga aaacatgttg tggcacaaac
    12481 ttccgaaatc tatctacttc cgccgtggct ccctgccaat cgcgctggat gaagtgatta
    12541 ctgatggcca caaacgtgcg ctcatcgtga ctgaccgctt cctgttcaac aatggttatg
    12601 ctgatcagat cacttccgta ctgaaagcag caggcgttga aactgaagtc ttcttcgaag
    12661 tagaagcgga cccgaccctg agcatcgttc gtaaaggtgc agaactggca aactccttca
    12721 aaccagacgt gattatcgcg ctgggtggtg gttccccgat ggacgccgcg aagatcatgt
    12781 gggttatgta cgaacatccg gaaactcact tcgaagagct ggcgctgcgc tttatggata
    12841 tccgtaaacg tatctacaag ttcccgaaaa tgggcgtgaa agcgaaaatg atcgctgtca
    12901 ccaccacttc tggtacaggt tctgaagtca ctccgtttgc ggttgtaact gacgacgcta
    12961 ctggtcagaa atacccgctg gcggactatg cgctgacccc ggatatggcg attgtcgacg
    13021 ccaacctggt tatggacatg ccgaagtccc tttgtgcttt cggtggtctg gacgcagtaa
    13081 ctcacgccat ggaagcttat gtttctgtac tggcatctga gttctctgat ggtcaggctc
    13141 tgcaggcact gaaactgctg aaagaatatc tgccagcgtc ctaccacgaa gggtctaaaa
    13201 atccggtagc gcgtgaacgt gttcacagtg cagcaactat cgcgggtatc gcatttgcga
    13261 acgccttcct gggtgtatgt cactcaatgg cgcacaaact gggttcccag ttccatattc
    13321 cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat tcgctacaat gcgaacgaca
    13381 acccgaccaa gcagactgca ttcagccagt atgaccgtcc gcaggctcgc cgtcgttatg
    13441 ctgaaattgc cgaccacctg ggtctgagcg caccgggcga ccgtactgct gctaagatcg
    13501 agaaactgct ggcatggctg gaaacgctga aagctgaact gggtattccg aaatctatcc
    13561 gtgaagctgg cgttcaggaa gcagacttcc tggcgaacgt ggataaactg tctgaagatg
    13621 cattcgatga ccagtgcacc ggcgctaacc cgcgttaccc gctgatctcc gagctgaaac
    13681 agattctgct ggatacctac tacggtcgtg attatgtaga aggtgaaact gcagcgaaaa
    13741 aagaagccgc tccggctaaa gctgagaaaa aagcgaaaaa atccgcttaa tcagtagcgc
    13801 tgtctggcaa tataaacggc cccttc
//
LOCUS       DAETPZ010000098        13798 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.098, whole genome shotgun sequence.
ACCESSION   DAETPZ010000098 DAETPZ010000000
VERSION     DAETPZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.098"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(511..1602)
                     /gene="ychF"
                     /locus_tag="K0W59_003302"
     CDS             complement(511..1602)
                     /gene="ychF"
                     /locus_tag="K0W59_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBI3024222.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1719..2303)
                     /gene="pth"
                     /locus_tag="K0W59_003303"
     CDS             complement(1719..2303)
                     /gene="pth"
                     /locus_tag="K0W59_003303"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBI3024223.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2581..2859
                     /gene="ychH"
                     /locus_tag="K0W59_003304"
     CDS             2581..2859
                     /gene="ychH"
                     /locus_tag="K0W59_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HBI3024224.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2914..4593)
                     /gene="dauA"
                     /locus_tag="K0W59_003305"
     CDS             complement(2914..4593)
                     /gene="dauA"
                     /locus_tag="K0W59_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HBI3024225.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4718..5665)
                     /gene="prs"
                     /locus_tag="K0W59_003306"
     CDS             complement(4718..5665)
                     /gene="prs"
                     /locus_tag="K0W59_003306"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBI3024226.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5816..6667)
                     /gene="ispE"
                     /locus_tag="K0W59_003307"
     CDS             complement(5816..6667)
                     /gene="ispE"
                     /locus_tag="K0W59_003307"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBI3024227.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLKYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6667..7290)
                     /gene="lolB"
                     /locus_tag="K0W59_003308"
     CDS             complement(6667..7290)
                     /gene="lolB"
                     /locus_tag="K0W59_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HBI3024228.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7504..8760
                     /gene="hemA"
                     /locus_tag="K0W59_003309"
     CDS             7504..8760
                     /gene="hemA"
                     /locus_tag="K0W59_003309"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HBI3024229.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKNLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8802..9884
                     /gene="prfA"
                     /locus_tag="K0W59_003310"
     CDS             8802..9884
                     /gene="prfA"
                     /locus_tag="K0W59_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBI3024230.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9884..10717
                     /gene="prmC"
                     /locus_tag="K0W59_003311"
     CDS             9884..10717
                     /gene="prmC"
                     /locus_tag="K0W59_003311"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBI3024231.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10714..11106
                     /locus_tag="K0W59_003312"
     CDS             10714..11106
                     /locus_tag="K0W59_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HBI3024232.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11110..11919
                     /locus_tag="K0W59_003313"
     CDS             11110..11919
                     /locus_tag="K0W59_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HBI3024233.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEEPISEMIRAQINNIAHKHIVLH"
     gene            11955..12809
                     /gene="kdsA"
                     /locus_tag="K0W59_003314"
     CDS             11955..12809
                     /gene="kdsA"
                     /locus_tag="K0W59_003314"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HBI3024234.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12958..13065)
                     /locus_tag="K0W59_003315"
     CDS             complement(12958..13065)
                     /locus_tag="K0W59_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBI3024235.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13494..13601)
                     /locus_tag="K0W59_003316"
     CDS             complement(13494..13601)
                     /locus_tag="K0W59_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBI3024236.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         3418 a         3486 c         3553 g         3341 t
ORIGIN      
        1 attttcgctt gatgtatctg cataacaaaa acaatatgcg ccatttttgc attactaata
       61 agaaaaagca aaaaacatcg atattcattt ttggtcaatg gattttctgg cctatcgtta
      121 ttattgtgct actggttatc attttcatct tgccaggatc gcacgtaatc acgttattcg
      181 gggactttat ctgcgggata ttacccttca ggtaagtcct tttctcgcca gcatattgga
      241 gcgagatcaa tattgatcag ctgtgaagag ggctgatgtg tagtggaaca aaaactgacg
      301 ttatatcact gctaatgaga tatcagtcat ctgaacactt atgtctttaa ttattctcgt
      361 ggttcactgt aggcaataag cacaaaagtg taggatgtta caagaatgat taggactcgg
      421 tgaaatgaaa aatccacgca attgcgtgga ttttatatac ttttgcgctc ttcatgagat
      481 ttagcgaaac ctcatgagac aataaattaa ttagacgttg aaaaggaagt tcatcacatc
      541 gccatctttc acgatgtaat ctttaccttc tgcacgcatt ttgcctgctt ctttcgcgcc
      601 ttgttcacct ttgtaagtga tgaaatcttc aaacgagatg gtttgtgcac ggataaagcc
      661 tttttcaaaa tcagtatgga ttttgcccgc tgcctgcggc gcggttgctc caaccggaat
      721 ggtccatgca cgcacttctt tcaccccagc ggtgaagtaa gtttgcaggt tcagcagctt
      781 ataaccggca cggatcacac ggttcaggcc cggttcttcc agcccaagct cctgcataaa
      841 ctcgtcacgt tcttcgtcgt ccagttcggc aatgtctgct tcaacagcag cacaaaccgg
      901 aaccacaaca gaaccttctt tcgccgcgat ttcacgcacc tggtcaagat atgggttgtt
      961 ttcaaaaccg tcttcgttga cgttggctat gtacattgtt ggtttcagcg tcaggaagct
     1021 caggtaacga atagccgctt tctcttcagc gcttaaatcc agcgcgcgca gcatacctgc
     1081 gttttccagc tggggcaggc atttttccag gaccgccagc tcagctttcg cgtctttatc
     1141 gccacctttg gctttcttct gtacgcgatg aatcgcacgt tcgcaggtgt cgaggtctgc
     1201 cagcgccagt tcggtgttga taacttcaat atcgtcagcc gggttaactt tgccggaaac
     1261 gtgaatgatg ttgtcatttt caaagcagcg aacaacgtga ccgatcgctt cggtttcacg
     1321 gatgttggtc aggaactggt tacccagacc ttcgcctttc gatgcgcctt ttaccagacc
     1381 ggcgatatcg acaaattcca tggtcgtggg aagcgtacgt tgcggtttta cgatttcagc
     1441 cagttgatcc aggcgagggt caggcattgg tacgacgcct gtgttcggct caatggtgca
     1501 gaatggaaag ttggccgctt caataccggc tttggtcagc gcgttgaaca gggtagattt
     1561 cccgacgttg ggcaaaccga cgataccgca tttgaatccc atgatttaaa tcaccttaat
     1621 atcttaataa tcaacctgtt attgctaaca gattgcagaa atggaaataa ctttgcctat
     1681 tatacacggc actcggcaaa aatgccgcag acaacgactt attgcgcttt aaaggcgtgc
     1741 aatcggttcg ttgctttggt caagccatct gtaaaccaca tttcagtaca acgcgccgct
     1801 tcgtcgatgg cttcatcaat taacttctgt tcactaacag gcggtttgcc taacacaaaa
     1861 ccgacaactt tatttttatc gcccggatga ccgattccga tgcgtaaacg gtgaaagtta
     1921 gggttattac ccaatttact gatgatgtct ttcagtccat tgtgaccacc atggccaccg
     1981 cccaacttaa atttggcgac gccaggaggc agatccagtt cgtcgtgggc caccagaatt
     2041 tcgtccggat taatgcggaa aaaactggcc atcgccgcaa cggctttgcc gctgagattc
     2101 ataaatgtag tcgggactaa caggcggaca tcttcgcctc caagggtgac tcgcgaagta
     2161 taaccaaaga atttagcctc ttcgcgcagc ggagcgcgca aacgctctgc cagtaagtca
     2221 acgaaccagg caccagcatt atgtcgcgtt gcggcgtatt cagcaccggg gttcgccagg
     2281 ccgacaatca atttaatcgt cacgtttttt tgtcctgagt gtgtacataa ctggcgcgta
     2341 gtttactggt tgcggccccg cttgacaaaa aactgcgtat caaatgcaga taacgtaata
     2401 attgcctgag tggactatta gaaagtcaag gtgttcaggc gtttatttgt aaagttttgt
     2461 tgaaataagg gttgtaattg tgattacgcc cgcacataac ccacagggtg ttgtctatac
     2521 tttacacata aggaagaggg gtattccctg ttacaaccca gaaagttccg gaggtgacat
     2581 atgaaacgca aaaacgcttc gatactcggt aacgtgctca tggggttggg tctggtggta
     2641 atggtggtcg gcgtggggta ttcaatcctc aaccagttac cacagtttaa tatgccccag
     2701 tatttcgcac atggtgcagt gctaagtatt ttcgtcggtg ccattctctg gctggcgggt
     2761 gcccgtgttg gcgggcatga acaggtgtgc gaccgttact ggtgggttcg tcactatgac
     2821 aaacgttgcc gccgtagcga taatcgccgt catagctaac aaataatgca gtttgctgac
     2881 cagtcagttt gcgctgactg gtcaatctcg tacttataaa tccgccatcg ccgcgcgacg
     2941 attcgggaag aacgcgaggc gtcccgggat cggttgaatg cctgcgcgag ccatagttcg
     3001 cagtggctgg aattccacgt tgcacacgcg cagttcacat ccttcgggca gacgcttcac
     3061 aaaacgctgg aacgcatcaa gaccaccagc atcaagtacc ggaacggcat cccacttcag
     3121 aatcacaatc cgtttgcctt caagacgtga ctccaggtcc gtgaataagc cttcagcagc
     3181 agcaaaaaac agcgggccaa taacgcgcag aaccaggaca tcgtctggaa catctacgac
     3241 taccggtgcc aggcgagtca tacgtgcgat acgacgcata aacagcagcg atgccagcac
     3301 gatccccacg ctgatggcaa taaccatatc aaacagcacg gtcagtgaca tacacagcag
     3361 catgacgatg atgtcatctt tcggcgcatg acgcagcaag tcgaccactt tgtgcgcttc
     3421 actcatgttc cacgccacca tcaacagcag ggctgccatg gcggaaagcg gcagccagga
     3481 gagcagtggt gccagtacca gcagggcgag aataaccaga atagagtgga tcaccgccga
     3541 gataggggaa gttgccccgg cacggacgtt agcggcagaa cgcgcgatgg cagcagtagc
     3601 ggtaatacca ccaaagaatg gagcgatgat attccccagc ccctgaccga ccagttcact
     3661 attcgccttg tgcttagtcc cggtcatacc atccagtacc acagcgcaga gcagagattc
     3721 gatcgccccg agcattgcca ttgagaatgc cgcaggcagc agggtgcgaa tagaatccca
     3781 ggttagcgtg aattctgaat taggcagatc ccacggcagc accagttgcg gcagcagttg
     3841 cggaataccg ttaccctgag aaccatcggc caggacgtag tggaattgcg aaccgatggt
     3901 agcaacatgt ccgccgagca ggttaacaat ccccatcacc gcgcaaccag ccagcaaggc
     3961 cggaaggtga ccgggtaaac gaatgcccag acgcggccag aaaaccagaa tgccaagcgt
     4021 cacaatgcca atggcagcat cacccacatt aatggtcggc agcgccataa ataatgcgcc
     4081 gactttttgt agataatgtt ccgggacatg ggccatttgc agaccgagaa aatctttaat
     4141 ctgcatggta ccgatggtga tcccgatacc cgaggtgaaa cctaaggtga cggaaaccgg
     4201 aatatactca atcagacgac caaagcgtgc cagccccatc agaatcaaaa agattcccga
     4261 caacaaggtc gcaaccagca gtcctgccag tccaaactgt tgcgaaacgg gatagagaat
     4321 taccacaaat gccgcagtcg gaccggaaac gctaaagcgt gacccacccg ttagagcaat
     4381 gacaatcccc gcaacagctg cggtatataa accgtactgg ggtgccacac cactaccaat
     4441 agccaacgcc atcgccagcg ggatagcaat aatcccgacg gttatcccgg caatcaggtc
     4501 acgggtaaac cgtgcggcag tatatttttc tttccagcaa gcgtcgatca gagcgcggaa
     4561 aggcatcaca tgtgaggaaa atattttgtt cacaataatg tttcatccgt gagcgcatca
     4621 tctgtcaact aaatggcagg tgaaggaggc ataggtcata caaatggata ttacagacaa
     4681 aaaaacccgc cgcagcgggt ctttgagccg ggttcgatta gtgttcgaac atggcagaga
     4741 tcgattcttc gttgctgata cgacgaatcg cttcggccag catacctgac agggtcagag
     4801 tacgcacgtt cggcagtgat ttgatttcat cgctcagcgg aatggtatcg cagacaacga
     4861 cttcatcaat tacagagtta cgcaggttgt tcgccgcgtt gccagagaag atcgggtgag
     4921 tcgcgtacgc aaatacacgt ttagcaccac gttctttcag cgcttcagca gctttacaca
     4981 gcgtaccgcc agtgtcgatc atatcatcga ccagtacgca gtcacgacct gcaacgtcac
     5041 cgatgatatg catcacctgg gaaacgttcg cacgcggacg acgtttgtcg atgattgcca
     5101 tatcggtatc gttcagcagc ttagcgatag cgcgggcacg cacaacgccg ccgatgtccg
     5161 gagaaaccac aattgggtta tccagattca gctgcagcat gtcttccagc aggatcgggc
     5221 taccaaatac gttatcaacc ggaacgtcga agaaaccctg aatctgttca gcgtgcagat
     5281 ccactgtcag cacacggtca acaccgacgc tggagaggaa gtctgcaacc actttcgcag
     5341 tgattggtac acgagcggaa cggacgcgac ggtcctggcg cgcatagcca aagtagggga
     5401 taacagcggt gatacgacct gcggaagcac gacgcagggc atcaaccata acgactaatt
     5461 ccatcaggtt gtcgttagta ggggcacaag tggactggat gatgaaaata tcaccaccgc
     5521 gtacattttc gttaatttgt acgctgactt cgccgtcgct aaagcgacct acagcggcgt
     5581 cgccgagtga agtgtacagg cggttggcaa tacgttgtgc tagtaccggg gtggcgttac
     5641 cagcaaaaag cttcatatca ggcacgagaa gaacctcagg catgcgtcca ttggtggaaa
     5701 gaatctgccg aaaactgtgc gggccaggca tgatcctttc caggcggtgt attaaagagc
     5761 gcgatgcaac gtctggaaca aggtgacgtt gtcaccgaaa ctcagcttgc ccggcttaaa
     5821 gcatggctct gtgcaatggg gaaagattaa cgcctttcgc cacaaagcca ttgagccatt
     5881 ccggggcttg ctctagcacc tggcgggctt cagactctgt atcaaattca gcaaagacac
     5941 aggcccctgt cccagtcagg cgcgacgggg cgtattttaa cagccaggaa agcaccgcat
     6001 caacctcgcg aaaacgtttt cttgcgataa cctcgcaatc attgctgaat tcacatttta
     6061 gcaacgtttc tattgacctt tttggcgtat tgcgcgggag ttcaggatct ttaaaaatca
     6121 ccggagtcgg aatacttaca ccagggtgcg ccaccagata ccacttctct ggcggatcca
     6181 ccggcgttag tatttcacca acgccttcgg caaacgcggc atgcccccga acaaagacag
     6241 gaacatctgc gcccagcgtc agccccattt ccgccagctc atccatgctt agcccgcatt
     6301 gccagagatg atttaatgcc accaggaccg tcgcggcatt ggatgaacca ccgccgagac
     6361 cgccgcccat cggcaaacgc ttgtcaatgc tgatattcgc accgcttccc gtcggaagac
     6421 gcccgctgtc tgccgcagtt ttcatcaaca accgcgctgc gcgaacgatc aggttatctt
     6481 catgttccac gccttcaacg ggcgttaaca gacgaatatc cccatcgtca cgaagctcaa
     6541 tgctgatggt gtcgccgtaa tcaagaaact gaaacagcgt ttgcagcgtg tggtaaccat
     6601 ccgcacgctg accggtaatg tataaaaaca gattaagttt tgccggagag ggccactgtg
     6661 tccgcattat ttcactatcc agttatccat ttttaacttg atgcgttgac caccgtcggt
     6721 gagttccata ttggctggca tcgcaggttg cgttttggtg tcataaccac cataaacaac
     6781 cttccagttt ttgccattct ggctgtaggt aatttcgctc aggcggtact ggtcgtccag
     6841 tttgtagtcg gttgcatcac ccggtaaacc taaaatccac tggcgcaagc tgttgagcgg
     6901 aattggcatt ccggtcaatt tgccaatcat ctcttcggcg tcatcggcgg tataacgctg
     6961 acctttattg tcgactaact gcacgttacc cggttgagca ttcagctcca gttccgtgct
     7021 gcccaatggg ttagtgagca gcagacggta gcgatcctgg ccggtttgct gccagaaaaa
     7081 gcgggcgtac actttttgtt ggtcagaaat ataagcgaac gcgccgcgag tctgatactg
     7141 attaagattg cgcacgtctt gctgatgctg acgccattgt ggcgaatccg ggcttttgcc
     7201 aggacctttg ggcgtggtaa cggaacaggc agtgagcaca agagcagcca gcggtagcag
     7261 gcggataaga cgaaaatcgg gcaggggcat agtgatgaca agtccttgag atacgttgca
     7321 gttataaccc ttaatgctag cgttaccgtc cgctatcgtc tatgttcaag ttgtcttaat
     7381 tgccagaatc taacggcttt cggcaattac tccaaaaggg ggcgctctct tttattgatc
     7441 ttacgcatcc tgtatgatgc aagcagacta accctatcaa cgttggtatt atttcccgca
     7501 gacatgaccc ttttagcact cggtatcaac cataaaacgg cacctgtatc gctgcgagaa
     7561 cgtgtatcgt tttcgccgga taagctcgat caggcgcttg acagcctgct tgcgcagccg
     7621 atggtgcagg gcggcgtggt gctgtcgacg tgcaaccgca cggaacttta tcttagcgtt
     7681 gaagagcagg ataacctgca agaggcgtta atccgctggc tttgcgatta tcacaatctt
     7741 aatgaagaag atctgcgtaa aaacctctac tggcatcagg ataacgacgc ggttagccat
     7801 ttaatgcgtg ttgccagcgg cctggattca ctggttcttg gggagccgca gatcctcggt
     7861 caggttaaaa aagcgtttgc cgattcgcaa aaaggtcata tgaaggccag cgaactggaa
     7921 cgcatgttcc agaaatcttt ctctgtagcg aaacgcgttc gcactgaaac agatatcggt
     7981 gccagtgctg tgtctgtcgc ttttgcggct tgtacgctgg cgcggcagat ctttgaatcg
     8041 ctctctacgg tcacagtgtt gctggtaggc gcgggcgaaa ccatcgagct ggtagcgcgt
     8101 catctgcgcg aacataaagt acagaagatg attatcgcca accgcactcg cgaacgtgcc
     8161 caaattctgg cagatgaagt tggcgcggaa gtgattgccc tgagtgagat cgacgaacgt
     8221 ctgcgcgaag ccgatatcat catcagttcc accgccagcc cgttaccgat tatcgggaaa
     8281 ggcatggtgg agcgcgcatt aaaaagtcgt cgtaaccaac caatgctgtt ggtggatatt
     8341 gccgttccgc gcgatgttga gccggaagtt ggcaaactgg cgaatgctta tctttatagt
     8401 gtggacgatc tgcaaagcat catttcgcac aacctggcgc agcgtaaagc cgcagcggtt
     8461 gaggcggaaa ctattgtcgc tcaggaaacc agcgaattta tggcgtggct gcgagcacaa
     8521 agcgccagcg aaaccattcg cgagtatcgc agccaggcag agcacgttcg cgatgagtta
     8581 accgccaaag cgttagcggc ccttgagcag ggcggcgacg cgcaagccat tatgcaggat
     8641 ctggcatgga aactgactaa ccgcttgatc catgcgccaa cgaaatcact tcaacaggcc
     8701 gcccgtgacg gggataacga acgcctgaat attctgcgcg acagcctcgg gctggagtag
     8761 cagtacatca ttttcttttt ttacagggtg catttacgcc tatgaagcct tctatcgttg
     8821 ccaaactgga agccctgcat gaacgccatg aagaagttca ggcgttgctg ggtgacgcgc
     8881 aaactatcgc cgaccaggaa cgttttcgcg cattatcacg cgaatatgcg cagttaagtg
     8941 atgtttcgcg ctgttttacc gactggcaac aggttcagga agatatcgaa accgcacaga
     9001 tgatgctcga cgatcctgaa atgcgtgaga tggcgcagga tgaactgcgc gaagctaaag
     9061 aaaaaagcga gcaactggaa cagcaattac aggttctatt actaccaaaa gatcctgatg
     9121 acgaacgtaa cgccttcctc gaagtccgtg ccggaaccgg cggcgacgaa gcagcgctgt
     9181 ttgcaggcga tctgttccgt atgtacagcc gttacgccga agcccgccgc tggcgggtag
     9241 aaatcatgag cgccagcgag ggtgaacatg gtggttataa agagatcatc gccaaaatta
     9301 gcggtgatgg tgtgtatggt cgtctgaaat ttgaatccgg cggtcatcgc gtgcaacgtg
     9361 ttcctgctac ggaatcgcag ggtcgtattc atacttctgc ttgtaccgtt gcggtaatgc
     9421 cagaactgcc tgacgcagaa ctgccggaca tcaacccagc agatttacgc attgatactt
     9481 tccgctcgtc aggggcgggt ggtcagcacg ttaacaccac cgattcggca attcgtatta
     9541 ctcacttgcc gaccgggatt gttgttgaat gtcaggacga acgttcacaa cataaaaaca
     9601 aagcgaaggc gctttcagtg ctcggtgctc gcatccacgc tgctgaaatg gcaaaacgcc
     9661 aacaggccga agcgtctacc cgtcgtaacc tgctggggag tggcgatcgc agcgaccgta
     9721 accgtactta caatttcccg caggggcgcg ttaccgatca ccgcatcaac ctgacgctct
     9781 accgcctgga tgaagtgatg gaaggtaagc tggatatgct gattgaaccg attatccagg
     9841 aacatcaggc cgaccaactg gcggcgttgt ccgagcagga ataatggaat atcaacactg
     9901 gttacgtgaa gcaataagcc aacttcaggc gagcgaaagc ccgcggcgtg atgctgaaat
     9961 cctgctggag catgttaccg gcagagggcg tacttttatt ctcgcctttg gtgaaacgca
    10021 gctgactgac gaacaatgtc agcaacttga tgcgctactg acacgtcgtc gcgatggtga
    10081 acccattgct catttaaccg gggtgcgaga attctggtcg ttgccgttat ttgtttctcc
    10141 agcgacctta attccgcgcc cggatacgga gtgtctggtg gagcaggcac tggcgcggtt
    10201 gcctgaacaa ccttgccgta ttctcgatct cgggacgggt accggggcga ttgcgcttgc
    10261 gctggctagc gagcgcccgg actgcgaaat tatcgctgta gatcgtatgc ctgatgctgt
    10321 ctccctggca caacgtaatg cccagcatct ggcgatcaaa aatatccaca ttctgcaaag
    10381 cgactggttt agcgcgctag ccgggcagca gtttgcgatg attgtcagca atccgccgta
    10441 tattgacgag caggacccac atcttcaaca aggcgatgtc cgctttgagc cgctcactgc
    10501 gctggttgcg gcagacagtg gaatggcaga catcgtgcat atcatcgaac agtcgcgtaa
    10561 cgcgctggta tccggcggct ttctgcttct ggaacatggc tggcagcagg gcgaagcggt
    10621 gcgacaagca tttatcctcg cggggtatca tgacgtcgaa acctgccgtg actatggtga
    10681 taacgagcgc gtaacgctcg gccgctatta tcaatgacaa gtttttctac actgcttagt
    10741 gttcatctta ttagtatcgc gctttctgtt gggctattaa ccttacgttt ctggctacgt
    10801 tatcagaagc atccacaggc atttgctcgc tggacgcgca ttgtgccgcc ggttgtcgat
    10861 acggtgttat tgttaagcgg cattgcgttg atggctaaag cgcacatcct gccattttcc
    10921 gggcaggcac agtggctgac tgaaaagctg tttggagtta tcatttatat cgttttgggt
    10981 tttattgcac tcgattatcg tcgtatgcac agtcagcagg cgcgcattat tgccttcccg
    11041 ctggcgttgg tggtgctgta catcatcatt aaactcgcca ccacaaaagt accgttactg
    11101 gggtaagtca tgagatcgtt agctgatttc gaatttaata aagcgccatt gtgcgaaggc
    11161 atgatcctgg cttgcgaagc aatccgccgc gattttccct cgcaagatgt ttacgacgaa
    11221 ctggagcgtc tcgttagtct ggcgaaggaa gaaatcagcc agcttctgcc tttagaagag
    11281 cagctggaaa aactgatcgc gctgttttac ggcgactggg gatttaaagc ctcacgcggc
    11341 gtttatcgtc tttccgatgc attatggctg gaccaggtgt taaagaatcg acagggcagt
    11401 gcggtatcat taggtgcggt tttattatgg gtcgcgaatc gtctcgattt gccgctgctg
    11461 ccggtgattt tccctacgca gctgatattg cgcattgaat gtccggatgg cgaaatttgg
    11521 ctgattaatc cttttaacgg tgaatcgtta agcgaacata tgctggacgt atggttaaag
    11581 ggaaatatca gcccgtcggc ggaactgttt tatgaagacc ttgatgaagc tgataacatt
    11641 gaggtaatcc gcaaattgct ggatacactc aaagcctcgt tgatggaaga aaatcagatg
    11701 gagctggcgt tacgcaccag cgaagcttta ttacaattca accctgaaga tccctatgaa
    11761 attcgcgatc gcgggttgat ttatgcgcaa ctggattgcg aacacgttgc gttgaacgat
    11821 ttaagttatt tcgttgaaca gtgtccggaa gagccgatca gcgaaatgat ccgtgcgcaa
    11881 ataaataaca tcgcgcataa acatattgtg ctgcattaat taatcgacat tttactcaag
    11941 attaaggcga tcctatgaaa caaaaagtgg ttagcattgg cgacatcaac gtagcaaatg
    12001 acctgccgtt cgtactgttt ggcggtatga acgtgctgga atctcgcgat ctggcgatgc
    12061 gcatttgcga gcactacgta actgtgaccc agaaactggg tatcccttac gtgttcaaag
    12121 cctcttttga caaagccaac cgctcctcca tccactctta tcgtggaccg ggcctggaag
    12181 aagggatgaa aatcttccag gagttgaagc agacttttgg cgtgaaaatt atcaccgacg
    12241 ttcacgaacc aagtcaggca cagcccgttg ctgatgtcgt ggatgtgatt cagttgccgg
    12301 cgtttcttgc tcgccagact gacctggttg aagccatggc gaaaaccggt gcggtaatta
    12361 acgtcaagaa accacagttt gtcagcccgg gacagatggg taatatcgtt gataaattca
    12421 aagaaggcgg caacgaaaaa gtgattcttt gcgatcgtgg tgctaacttc ggctatgaca
    12481 acctggttgt cgatatgctg ggcttcagca ttatgaagaa agtgtctggt aactcgccgg
    12541 tgattttcga cgtgacccac gcactgcaat gccgcgatcc gtttggcgca gcttccggtg
    12601 gtcgtcgtgc tcaggtggct gagctggcac gagccggtat ggcggtaggt ctggcggggc
    12661 tgtttattga agcgcatccg gatccggaac atgcgaaatg tgatggtcca tccgcgctgc
    12721 cgctggctaa actggaaccg ttcctcaagc agatgaaagc gattgatgat ctggtgaaag
    12781 gtttcgaaga actggatacc agcaagtaat cttttttgct tgaaaaataa agtcttagcg
    12841 ttctgcgtta agactttttt catgggtgcc agatacaaaa aaggccgcag gctgttaccc
    12901 ctgcggccgg tttcgggcgc atattgccat cacggcagcc tgacgcccgt tttcacctta
    12961 cttccggtta cgccaccagc cgacaatcgc tgcggtaata attcccgcca ggatcggtgc
    13021 tgccaggtcg tgccagaaaa tcatggcaaa ctgcgcgagc gtcatatagc cgccttgttg
    13081 taatgacaac attttgcggc tattcttgaa ttgttctggt tcaagattag cccccgttct
    13141 gttgtcaggt taaacctctc aacgtgcggg ggttttctct ttccagcaac caatgccacc
    13201 agggataaag cccccgcaac attgcgcctc accggataac gccggcttga tgtggatact
    13261 acgtctcaat tcatcttcac ttcatccctg aaatgtttgc aatgaagagt gcattccggt
    13321 ttttcaacag ctgttacagt cgtttcatga gtgctctgga tgatgcttcc agctcgggtt
    13381 gccaatattt acttgtggaa gagataaaga caaaaatggc cgcaggcatg ttacccatgc
    13441 ggccggtttc gggcgcatat tgccatcacg gcagcctgac gcccgttttc accttacttc
    13501 cggttacgcc accagccgac aatcgctgcg gtaataatcc ccgccaggat cggtgctgcc
    13561 aggtcgtgcc agaaaatcat ggcaaacttc gcgagcgtca tatagccgcc ttgttgtaat
    13621 gacaacattt tgcggctatt cttgaattgt tgatggttca agattagccc ccgttctgtt
    13681 gtcaggtttt acctctcaac gtgcggtaat gccagtcagt taagcaactg actggctctt
    13741 tttcggggct gtggggtatt tccagggcct ctcctttacc actctcggga aggctctt
//
LOCUS       DAETPZ010000099        13530 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.099, whole genome shotgun sequence.
ACCESSION   DAETPZ010000099 DAETPZ010000000
VERSION     DAETPZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.099"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..898)
                     /locus_tag="K0W59_003317"
                     /pseudo
     CDS             complement(1..898)
                     /locus_tag="K0W59_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            1120..1920
                     /locus_tag="K0W59_003318"
     CDS             1120..1920
                     /locus_tag="K0W59_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBI3024237.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            1998..2768
                     /locus_tag="K0W59_003319"
     CDS             1998..2768
                     /locus_tag="K0W59_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBI3024238.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2816..4174)
                     /gene="mltD"
                     /locus_tag="K0W59_003320"
     CDS             complement(2816..4174)
                     /gene="mltD"
                     /locus_tag="K0W59_003320"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HBI3024239.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4246..5001)
                     /gene="gloB"
                     /locus_tag="K0W59_003321"
     CDS             complement(4246..5001)
                     /gene="gloB"
                     /locus_tag="K0W59_003321"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HBI3024240.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5035..5757
                     /locus_tag="K0W59_003322"
     CDS             5035..5757
                     /locus_tag="K0W59_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBI3024241.1"
                     /translation="MKPARVPQTVVAPDCWGDLPRGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5754..6221)
                     /gene="rnhA"
                     /locus_tag="K0W59_003323"
     CDS             complement(5754..6221)
                     /gene="rnhA"
                     /locus_tag="K0W59_003323"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HBI3024242.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6286..7017
                     /gene="dnaQ"
                     /locus_tag="K0W59_003324"
     CDS             6286..7017
                     /gene="dnaQ"
                     /locus_tag="K0W59_003324"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HBI3024243.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7150..7226
                     /locus_tag="K0W59_003325"
     tRNA            7150..7226
                     /locus_tag="K0W59_003325"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7184..7186,aa:Asp,seq:gtc)
     gene            complement(7357..8403)
                     /locus_tag="K0W59_003326"
     CDS             complement(7357..8403)
                     /locus_tag="K0W59_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HBI3024244.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8414..9409)
                     /gene="tssG"
                     /locus_tag="K0W59_003327"
                     /pseudo
     CDS             complement(8414..9409)
                     /gene="tssG"
                     /locus_tag="K0W59_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9406..11289)
                     /gene="tssF"
                     /locus_tag="K0W59_003328"
                     /pseudo
     CDS             complement(9406..11289)
                     /gene="tssF"
                     /locus_tag="K0W59_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
     gene            complement(11305..11799)
                     /gene="tssE"
                     /locus_tag="K0W59_003329"
     CDS             complement(11305..11799)
                     /gene="tssE"
                     /locus_tag="K0W59_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HBI3024245.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11796..12626)
                     /locus_tag="K0W59_003330"
     CDS             complement(11796..12626)
                     /locus_tag="K0W59_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
                     /protein_id="HBI3024246.1"
                     /translation="MTNNNELPITLSALLRDYSVVEGIQMAEQQVRMHPAQASRRHSL
                     FQLLCVAGDWSRALQQIQLCARMDANYTREAQVFGELIRCEIYRHACFQGEQRPGVIL
                     PPPAWMEDLLTTLACNARGEAQEADAHRSRALEAITDTSGQWNGGAFDWISDSDSRTG
                     PVLELIAGGAYIWLPFSQICSLKSPRPAHLIDLIWKPVNVTLNNGDTHSAWLFPRYSG
                     SEKAADSLRLCRETVWQDGPGETLVQALGQRVWLTGHGDISLLDLSTCTFNTAEGSDA
                     "
     gene            complement(12613..12990)
                     /gene="tagK"
                     /locus_tag="K0W59_003331"
     CDS             complement(12613..12990)
                     /gene="tagK"
                     /locus_tag="K0W59_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
                     /protein_id="HBI3024247.1"
                     /translation="MLSPEQDLNRHADPFPFNEEPSSQDLDSLRDLVTEAETLQDMVT
                     GGLSIDAILNVLDATGEDETIWPVEKTPPDILHLLSPEYAPETAHNTVLPDLTRKEHR
                     IIGIDNHYRINPAQHGEIYHDKQ"
     gene            complement(13227..13397)
                     /locus_tag="K0W59_003332"
     CDS             complement(13227..13397)
                     /locus_tag="K0W59_003332"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HBI3024248.1"
                     /translation="MNDDVKIALTLTRHEEAWWIINQSTEYCCTVNDQIVEPHHRMRL
                     NEGDLIEWGLSS"
BASE COUNT         3094 a         3524 c         3612 g         3300 t
ORIGIN      
        1 cctccgggag ctgtttttac atgctcgcta aggaaatcga taaaagcccg gatgcgcgta
       61 cttaccgcac ggtcgctgta atagacggca ctgaatggca tttccactgg caacacttta
      121 tctgccatta actccaccaa ttatccgcga gcgatttctt tgtcgatcat gtagtcggac
      181 aaacacgcaa tcccgttgcc actcaggcaa agctgtttca gtgtttcccc actattggat
      241 gacaaaccgt acttcacctc atgtaattgt ccatcactac aggctatcgg ccaggtattg
      301 agggaagcgg gttcagtgaa tcccaggcaa acatgttgct ttaaatcgtc gatcgtttct
      361 ggcttcccgt agcgggaaat ataatcgggg gaggcgataa tttttcgata actgttaaat
      421 aacggcctgg cacgtaagct ggagtccgtt aacgtaccag cgcgtatcgc gacatccact
      481 tttctttcga tcaaattaat aatcgtttcg gaggagacta gcgataaagt gacttccgga
      541 tagcgttcac ggaaaggctt aattaacggc atcagaaagt gcagcaccac tggagttgcg
      601 gcatcgatcc gtaatagtcc acgcggtgta ttacgcgtct ccataatttc tgattctgcc
      661 gctgccatct cctgcaaaat tgactgtacg cgacgaaaat aacgctcgcc ttcttccgtc
      721 aggctaagtt gccgcgtggt ccgattaagc aggctaacgc caagtttcat ttccagcttt
      781 ttcaccgccc ggcttaccgc tgagtttgct tgccctaaaa ttgttccgct gcccggctaa
      841 agctgccgct ttcgacgacc gaaacaaaaa tggcgagttc ttccgacgtg gctttcattt
      901 ttgctcctat tttgaattta tttgtcatta aaccatcagg atgcgtgata tgtcatccga
      961 tttaatgttc tcaataatga gcaaatttct gaccggtgta agcacttgct tacataacaa
     1021 tatacaattg ctcgttgaaa gagtgagcta aaatccctat aacagtagaa ccctcccgag
     1081 tgcggaaggg ttgacgtaat agaggtttca aagtcaaaag tgcgaaaaaa cacctatgcc
     1141 atgcgctatg ttgccggaca acctgcggaa aggatcttac cgccggggtc ttttgcgagc
     1201 atcggccagg cattaccacc tggagaaccg ttaagtaccg aagagcgtat tcggatcctg
     1261 gtgtggaaca tatacaaaca gcaacgcgct gaatggttgt cggtattaaa gaactacggc
     1321 aaagatgcac atctggtgtt attgcaggaa gcgcagacaa cgccagagtt agtacagttt
     1381 gcgaccgcta actatcttgc cgccgatcag gtgcccgctt tcgtgctgcc acaacatcct
     1441 tccggtgtaa tgaccctttc ggcggcgcat ccagtgtatt gctgcccgtt acgtgaacga
     1501 gaacccattt tgcgtctggc gaagtcggca ctggtgacgg tctatccatt acctgacacc
     1561 cgcctgttga tggtggttaa tatacacgcc gtcaacttca gtctgggcgt ggatgtctat
     1621 agtaagcagt tacttcctat tggtgatcag atagctcacc acagcggccc ggtcattatg
     1681 gcgggagatt tcaatgcctg gagccgtaga aggatgaacg cgttatatcg ctttgcgcgg
     1741 gaaatgtcgc tgcgccaggt gcgttttacc gatgatcagc gccgtcgggc gtttggccgc
     1801 ccgctcgatt ttgttttcta ccgtggtctg aacgtcagcg aagcttctgt attggttacg
     1861 cgcgcttccg atcacaatcc gctactcgtt gaattcagtc ccggcaagcc tgataaataa
     1921 ggtatgtcag gtctgccaca gggcagacca acgtttggcg ctgtcctttt atactcaaac
     1981 aacaaaagga cagcgcaatg acaacacaat cccaccatga ccacgtagaa aagcagttta
     2041 gctctcaagc ctgcgaatat ctgaccagta ccgtacatgc atccggacga gatttacagc
     2101 gcctggcggt gcgtctggct gattatcctg gtgcaagtgt acttgatatg ggctgcggag
     2161 cagggcatgc cagctttgtt gctgcgcaaa acgtgagcgc ggtggtggcg tatgacttat
     2221 ctgcccaaat gctggatgtc gtggcacaag ctgccgaagc ccggcaactg aaaaatatca
     2281 ccacccgcca gggatatgcc gaaagtctgc catttgccga taacgcattt gatattgtta
     2341 tcagccgtta ttctgcccat cactggcatg atgttggtgc agcactgcgt gaagtgaacc
     2401 ggatattgaa acctggcggt aggatgattg tgatggacgt aatgtctccg ggtcatccag
     2461 tgcgcgacat ctggttacag acggtagaag cattacgcga tacctctcac gtacgaaact
     2521 acgccagcgg tgagtggttg aggttaatca atgaagccaa tctgatagtt gataatttaa
     2581 ttacagataa gttaccgctg gaattttgtt catgggtcgc gagaatgcgt acgccagaag
     2641 cgttagtaga cgctattcgc atttaccaac agagcgcatc gacagaggtg agaacgtatt
     2701 ttgccttgca gaatgacggc tctttcacca gtgatatcat catggtagaa gcacataaag
     2761 cggcataaat aaaaaaggca ccgggggaat cggtaccttt ttattatctg gtttgtcagg
     2821 aatctggcat gttgttgttt ttcacaaaca acgtcagctt gtcgcctggt tgcagattcg
     2881 cagtgtcgct gttccagcgc atcacatctt tgatgttcac gccgtggcgt ttagcaatgc
     2941 ttgaaagcga atcgcctttg cgcacacgat acgtaatgct atcgctgttg tttgccagtc
     3001 gctgtgcgct actgcctgca ccaatcgtca aactttggcc tggcttcagc ttagatccgc
     3061 gcagtttgtt ccactgctgc aaatctttgg tgcttacgcc gagacgtgaa gcgatgcttg
     3121 aaagcgtgtc gccagagcgt acggtgtaaa cacggctgtt aagcggcgta ttgtcggcaa
     3181 ccagcgtcga ctgtacagca gcaatttcgc ctgaagcgag agattcacgc agttgatctg
     3241 catgcttctt tggcaccatc acgtactgcg gcccacttgc gcccagcgtg gagcctttca
     3301 cgccagcgtt gaatgtcttc agcttgctga cggaaatccc cgccatatct gcaaccttcg
     3361 ccatttcaac cgggctactc aggtgcacac gcgccagagc acggctttca tcggtcgttg
     3421 gcagacgtac gccataacgc ttgctgtttt tgagaatatc actcaatgcc agcattttag
     3481 gcacgtactg cttcgtttcc tgcggcaacg gtaacgacca gaagtccgtg gatttcccac
     3541 gcgctttgtt cgttttaatt gccttcatga cccgaccttc gccgctgtta taagccgcta
     3601 cggtcagaag ccagtcgccg tcaaacattt tgttcagacg ctgcatcata ttcagcgcgg
     3661 cagttgttga agcaacaaca tcgcgacgcg cgtcataatt gcgggtctgt ttcaaaccat
     3721 aattgcgccc cgtgctcgga atgatctgcc agatgcctgc ggcattggcg ccagacgttg
     3781 cgtgaggatc aaaagcgctc tccactatgg gtagtagtac cagttccata ggcatgttac
     3841 gttttttaac ttgccctgct atccagtaca tatacggctc tgcccgtaaa gttacatcgt
     3901 ggagatagct cttattgcgt aaatatttct gtttctgttc gcgaatccgg tcattttccg
     3961 gaattcccat ctttagctcg tcgccaatga aagcccacaa gtcaccatct ggcgcgatag
     4021 acgtcccatc gtccatccat cgtgcctgac ttgtaaactt tgctgcttcc ccttgaccag
     4081 ctgcagaaag gctctgtgcg tgctgttgaa cgttgccggt actctggcaa cccacgagca
     4141 ggacagaggc gagtaatatc gcttttgcct tcatgtgtgt gtcaatagtt gcttaaaaga
     4201 cgaccgatca taacggcgaa cggagccgat gacaagaaag ttttatcaga acctatcttt
     4261 ctttgacctt aaccatgcaa aacgctcttc aggttgttgc aataatgttt cttcattaat
     4321 tacattaatt aaatcaatat cttccgttct taaaaaaaca ttaatttgcc gctcattttt
     4381 cagaattacg ggtagtgtta tttgattttt tgcccgtaac tccttaactt tacgataata
     4441 atcatttatg gacaaatcgt gcggaaaaat actcaaagca aacttcatat ttgataacgt
     4501 atattcatga gcacaacata ccaatgtatc gtcaggtaac gcacttagct ttttaagtga
     4561 ttgatacatt tgtaaagctg tcccttcaaa caaccgacca cacccaccag aaaacagcgt
     4621 gtcgccgcaa aatagataag gtttactgaa gtaacagata tgtcctaaag tgtgacccgg
     4681 tgtggtaatt acactaaatt catgccctaa aacgaaggca gtttcgccat ctttgactac
     4741 ctgtgttgtt cccttatctt gtgtctcttg tggaccatac accacaattt gcggaaactt
     4801 ttccaccagt tcttttacgc cgccaacgtg atcgtggtga tggtgggtta gaaatatggc
     4861 ctccggttgc cagttattgg cggtaatggc gtttaatact ggctctgcgt ctccgggatc
     4921 gacaatcagg cagcgacctg cctcatcatt caaaacccag atgtaattgt catcaaaggc
     4981 gggaatactg ttaagattca tagattacct ctcagtgtaa aacggaaggt tgtgatgaaa
     5041 ccggcaagag tccctcaaac tgtcgtggct cctgattgct ggggcgattt gcccagggga
     5101 gagctttatc gcaaggcgct ggagcgccag ctcaacccgt ggttcactaa aatgtatggt
     5161 tttcatctgc ttaagattgg caatttaagc gcagaaatca attgcgaagc gtgcgcggtt
     5221 tctcatcaag tgaatgtttc tgcgcaagga atgcccgtcc aggtgcaggc ggatccactt
     5281 catcttcctt ttgccgataa atccgttgat atttgtctac tggcacatac cttgccgtgg
     5341 tgcaccgatc cgcatcgttt attacgtgaa gccgatcggg tactgattga tgatggctgg
     5401 ctggtgatta gtggcttcaa ccccatcagt ttaatgggat tacgcaaact tgtgccggta
     5461 ttgcgcaaaa cctcgcccta taatagccgg atgtttactc tgatgcggca actggactgg
     5521 ctctctttgt tgaattttga agtgctgcac gccagccgtt tccacgttct cccgtggaac
     5581 aaacacggag gaaaactatt gaatgcgcat attcctgcgc ttggttgctt acaacttatt
     5641 gttgcccgga aacggactat tcctttaacg ctaaatccga tgaaacagag taaaaacaag
     5701 ccacgaattc gccaggcggt tggagccacc cggcaatgtc gtaaaccaca ggcttaaact
     5761 tcgacttggt agcccgtatc ttccagtgtg ggattcatcg ccgcggcacg agccagttca
     5821 tcacagcgtt cgttttccgg gtgtccggca tggcctttaa cccattccca tttgatttga
     5881 tgctgcccca atgcagcatc aagacgttgc cagagatcga cattttttac tggttttttg
     5941 tctgctgttt tccagccacg ttttttccag ttatggatcc actgggtgat accctggcgg
     6001 acatactggc tgtcggtact caaaatgact tcgcaatgtt cttttaacgc ctccagcgcg
     6061 acaatagcgg ccatcaactc catacggttg ttggtggtgc gggtgtagcc tgcgctaaag
     6121 gttttctcgc gtccgcgata gcgtaaaata gcgccgtaac ccccaggtcc aggattaccc
     6181 agacacgaac catcggtgaa aatttctacc tgtttaagca tctctggtag acttcctgta
     6241 attgaatcga actgtaaaac gacaagtctg acataaatga ccgctatgag cactgcaatt
     6301 acacgccaga tcgttctcga taccgaaacc accggtatga accagattgg tgcacactat
     6361 gaaggccaca agatcattga gattggtgct gttgaagtag tgaaccgtcg cctgacgggc
     6421 aataacttcc atgtttatct caaacccgat cggctggtgg atccggaagc ctttggcgta
     6481 catggtattg ctgatgaatt tttgctcgat aagcccacgt ttgccgaagt agccgatgag
     6541 tttatggact atatccgcgg cgcggagttg gtgatccata acgcagcgtt cgatataggc
     6601 tttatggact acgagttttc gttgcttaag cgcgatattc cgaagaccaa taccttctgt
     6661 aaggtcaccg atagccttgc ggtggcgagg aaaatgtttc ccggtaaacg caacagcctc
     6721 gatgcgctat gtgcccgcta cgaaatagat aacagtaaac gaacgctgca cggggcatta
     6781 ctcgatgccc agatccttgc ggaagtttat ctggcgatga ccggtggtca aacgtcgatg
     6841 gcttttgcga tggaaggaga gacacagcag caacaaggtg aagcaacaat tcagcgcatt
     6901 gtacgtcagg caagtaagtt acgcgttgtt tttgcgacag atgaagagct ggcagcacat
     6961 gaagcccgtc tcgatctggt gcagaagaaa ggcggaagct gcctctggcg ggcataaata
     7021 catgtaaaag gcgctaaaaa tagcgacttg ggcgattttt gcagcaaacg attcaaaaga
     7081 tgagaaaaat cgttgacgaa ggtcgaggca atccgtaata ttcgcctcgt tcccaacgga
     7141 acacaacgcg gagcggtagt tcagtcggtt agaatacctg cctgtcacgc aggggggcgc
     7201 gggttcgagt cccgtccgtt ccgccaatat tcagaaagcc cgaatcagta atgattcggg
     7261 ctttcgtcgt tttggtgaag tgagaatttt tcactccgaa gtaaaacgga tatgatttaa
     7321 aaaatggcca gacattataa attctggcca cttctgttac tccagcgcat cccatttctc
     7381 cagcagttct actggaattt catttattac ctccgagacg cgttttccga ccaggcgttc
     7441 tgcctggcgc agcaaaggaa tggtcgggct gctcggttcg ctgtgctcaa accagcgacg
     7501 gatgacacgc aggcgttcca gcgcgtcgtt tcggtcgcgg atctgccggg gttctgcgcc
     7561 ggtcatcggt gatatttcct ccgcgatatg caccggtagc gcaatctgct ctgcctgcat
     7621 ctgttcagta tgaactatgg tggcgttttc cggctgctca gcctgtggtt gtggcaggat
     7681 ctccggcgtg gtgctctgca ctgctcccgg taacagggcc agcagttgcg tcagacggga
     7741 gaagtccggt gcctggtcat tcagcgtttc acgcgcatgg cgttgcagcc gctcagtggt
     7801 ctctgctgcc tggcgaaagg catccagcgg cagtgtgccg cgcgcttcca ggtctgccag
     7861 ttgctggcgt accgattctg gtgccagcgc atccgccggg cgtaatgcag atagcgaacg
     7921 ctccacatcc cgcacctgaa ggcgcatggc agcattattt gacagtgtaa tgccacgaat
     7981 atccgccatt acaccttcgt ggtccagcaa cgccgccagc gcgttactgc gtgccaccgc
     8041 agcatcttcg gcggtgatat cttcagtcgc gagcagttga ggatggatgg cgtccgggta
     8101 cgtggagcac aacgtttcca gcagcgtgag cgcctccgac agtcccctgg caccggcctg
     8161 ctggatacgg cagcgcagca ggacaaccag aatgcggata tctttgctgc gcatcagcag
     8221 acgatgggcg tcccgctcaa gctccgccca gttaatgttt tccggcgtac tgacaaaatc
     8281 gccatattgc gcttcttcac gcggtgcggc gcgggtgaac agcagcaggt attccggatc
     8341 gtactccaga tccgggccgc aggggcattc cgccgaaatt gggtcagaca ggaatatttc
     8401 catttaatta tcctcagtgc gccaggtagt gttctggttc gaaaatcatg cccgtcaccg
     8461 ggttgtcgtg catggcttta cccgcccatg cagtccaacc cagttggtac ggaccaccgg
     8521 taacagccgg aggggcggcg tggggtttta gctgtagctc aatttcccag cagtattcat
     8581 aaccactaaa ggcgcggacc agttctgtca ggacgggcag gttattgctg ccaggcagaa
     8641 aacgtcggta atcctccagc gtcagcgggc cgataaccag ccggaactta tactgcatgt
     8701 ccggcacggc ctggccgatg agtgcaccat tgccaataac ggaagagggg gcaggcatcc
     8761 ccagccgggt gatctcatcg tccgccacgg ctatccagtg caggacatac tcctgtatcc
     8821 ggaaaggtac accgaaatag tgcgccagcg tggtggccag accgtccgga ttgcgggact
     8881 cacgcaccag atgggccgat gccgccagac gagcgtgatc tggcaacggg ctgtcagcgc
     8941 tctcccgcaa atcctgcccg ctgaggctgg cgacataaaa ggcaaagcgg tcatgttgcg
     9001 gtttatccag accgccccca gcggactggg cgctgcgcca tgcctggtaa aactgcgtca
     9061 gccagcggtg atgaaaaaga tcagaaaaac gggtcagcgt cggatcgcgt cggctttcag
     9121 tccggttacg cgccagttcg gtgtagtgca gcggcagtgg accgttcggc ccccacagcc
     9181 cgaggctgta cagactgatg tgcaggcgcc cttcctgcca gcccacactg gcgatttctc
     9241 gtggcgcaaa ggtcagcgcg ggcgtttgtc cgaggcgaaa tttttccatc cgtggttgct
     9301 aggtgttgcc cgccggaacg gtacacaact gcacatccac gcggcgcatc aggttcagaa
     9361 aaccgtaatg ccaggggggt tcctgcgcct gagtgaaatc gttgctcata cgcttcccct
     9421 ctgtcccgcc ctgacaggcc aggtcataat tttgccgcgc tgcatgctgt gcagcgtcat
     9481 ctgcgagaag gtattaatgg aaacatggcg ggcgatatag tgttccagca ccaggccgaa
     9541 cagatacgga ctgatgccgg aaaaaccttc ttcatccacc gtcagttcac aactgacgcc
     9601 acgcccatag accagtagtc cggaaccggg cagacggcgg gtgaccgggg tcgttttaca
     9661 accaatcagg ctgcgcacct ggcgcgattg cggactgtca tgagcaggaa taaacaggtt
     9721 cagcagatct cgcaacgcct ggccgccggt acggtgatcc agatcggcca gcggcagata
     9781 attaaaagat aactggcgga tcaaccgcca tgccatctca cgttcagcca gcgggggctg
     9841 cggcgagcgt ggcgggcgga taagccctac gcccgccacc gggatggctg catccacggt
     9901 tagatcgtcc cggccattgc gggcaataag acagggcaga tcgcggttag tgaccatggc
     9961 cgtgatggtg atgtggcgca gattttccgg gtacggcgct tcatactggt caaccagcga
    10021 caggaacact tccgagcccg tatagggggt acgggtgccg taacggcggg cgttttctga
    10081 taagcggcgg ggctcccggc gcagggaaaa ataacgtccg tgattgcctt catcattatt
    10141 gcgggtgtgg tagagcgggc gaaaggccat cttccgtgtg gtgtcggttt ccagcccttc
    10201 cacttcctgg accgagaaca cctcataatc cagcggatgg gttctgtcca ccaccagatg
    10261 ctgttccgtc gtgctgtgcg tgacatcgat ccgggcggtg acgcggggaa acaggttgat
    10321 aaccggggta cagaacaggc tgaactgtgc cgcatttgtc tggtggatca gtcagtccgg
    10381 cggcaggcgg ttcagcagga taacaatctc cgccacgcca ccgtcaattt tctgtaatcc
    10441 ggcagacagg cctgtcgggg tgaaaaaata gaagcgttcc ggacaggcaa aatattcatg
    10501 cagcaggtta tgaccgtgaa acacgttcca cgccagcggc agcagcccct gatccggctc
    10561 caggccttcg tacataacgg gctgttgcag attcacatcc agcgcgccgt caaaatgccc
    10621 cggaatgcca gccagggtcg cgatggcgct ggtatgtaga agttctaaca ggtgtgaggc
    10681 cgtacgctcc tcgccgcaga gataaaacgg cagcctgtcc agtccggcaa gctgactgaa
    10741 cgttagttca ccaaaggtgc gcagggtaat acgcagcgcc ccggcgacgt gaatgttggc
    10801 cggcaggtag cggtgcaggg caggcatatc cggtggcgcg gcggtcagtc gcgcctcttc
    10861 gatggcgagc ggccacagcg ttacatcctg gctgctgcga aactggcagg cagtgctttc
    10921 gccttccgga atggcggaga aaaaggctgt gtcgcgcggg acggtaaatc ctttcgccag
    10981 atcgccttct tccgtatcgg gatgcaactg cgccacgctc attgagggcg tgggtgtgac
    11041 gtaattgggg ctgaccactt ccagcgggcg ctgggtgaaa cgggggaatt cagcgtcaat
    11101 ttttagctgc gtgcgggcgg tcaggaagct gaaggcctcg accattcgct ctgcatacgg
    11161 atcggcaata tccgttccct gcatgcccag ccgggcggcg actttgggat gacgggcggc
    11221 aaattcggct cccgtttccc gcagatagct cagttcgcgg ttgtagtatc ccagtagccg
    11281 tggatccatc ggtctctctc tgaattaaaa aaacgtaatg cgactctgct ccatatccag
    11341 cgcgctacgt acccggaatg cagtcggata cggctgcgtg aggatctcgc cgcgaatttc
    11401 aaactgtaat gtgttatggc cactctggcg gcgctcatcc tgaagtggga tgatttgcag
    11461 ggtggacgct ttcagccggg gttcaaaccg ggtgatggtt cgcaaaatgg ccctgcggat
    11521 atcgtcccac ttgtgctcat acagaaagct gcccgccagt ggcggcaggc cgtagttaag
    11581 tacggacgct gccgcgtacg gataacgtgc agcatcaata tcatcttcat ggctgacggt
    11641 gttgagcaga aatgacaaat cccggcggat gatctcctta atctgaaccg ggctgacgct
    11701 gataccgtgg tcgcgtttct ggtttggggc gtcgtcacac aggcggtcaa acagcgtggg
    11761 cagcaggtga ttgcctggac gctggcggga agcgctcacg cgtcgcttcc ttcagcggtg
    11821 ttaaaggtac aggtggacaa atccagcagg ctgatatcgc cgtgaccggt gagccacacc
    11881 ctctgaccca gcgcctgtac cagtgtctct cccggaccgt cctgccagac cgtttcccgg
    11941 cacagacgca gggagtcggc agccttttca gagccgctgt agcggggaaa cagccaggca
    12001 ctgtgtgtat cgccattgtt cagggtgacg ttgacgggtt tccagatcaa atcgatcaga
    12061 tgcgcaggcc gcggcgattt cagcgaacag atttgtgaaa acggcagcca gatatatgct
    12121 ccgcccgcga tcagttcgag taccggaccg gtacgggagt cgctgtcgct aatccagtca
    12181 aatgcgccgc cattccactg cccggaagtg tctgttatgg cctcaagcgc gcggctgcgg
    12241 tgagcatcag cttcctgcgc ttcaccacgg gcgttacagg cgagggtggt cagcagatcc
    12301 tccatccagg cgggcggcgg gagaataaca ccgggccgct gttcgccctg aaagcaggcg
    12361 tggcggtata tctcacagcg gatcagctcg ccaaatacct gcgcttcgcg agtataattt
    12421 gcgtccatcc gggcgcatag ctggatctgt tgcagtgccc gcgaccagtc gcctgccacg
    12481 cacaataact gaaacaggct gtggcggcgg gaggcctgcg ctgggtgcat cctgacctgt
    12541 tgctctgcca tctgaatgcc ctccaccacg gaataatccc gcagcagcgc cgacaaggta
    12601 atcggtagct cgttattgtt tgtcatgata tatctctcca tgttgtgccg ggtttatccg
    12661 gtaatggttg tcaatgccaa tgatgcgatg ctctttccgt gtcagatctg gcagtacggt
    12721 gttatgcgct gtttccgggg cgtattccgg ggaaagtaaa tgcaggatat cagggggagt
    12781 cttttcgaca ggccagatag tttcatcttc gccagtggca tccagtacat tgagaatagc
    12841 gtcaatgctt aacccaccag tgaccatatc ctgcaatgtc tcggcttcgg tgaccaggtc
    12901 acgcaatgaa tccagatcct gcgaggaggg ctcttcgtta aaaggaaaag gatcagcatg
    12961 gcgattaaga tcttgttcgg gagataacaa cgcctccggt attcctgata aagtcgggtc
    13021 agggcatttc ctttttccgc accttctccg gtggaggagg ctccatgaac aggaatgata
    13081 tcgaatggat tttgtgatgt cagatgctgc tgcctgaacc aatccagatc cagatattcg
    13141 gttattgctt ccgacgtgat ggcagtctgc gatatcgttt ttactgtgct caggtccgga
    13201 gatgatggat tgccatgtac aaggcactat gaagataacc cccattctat caggtcgcct
    13261 tcattcagac gcatacggtg atgaggttcc acaatctggt cgtttaccgt acagcaatat
    13321 tcggtagact gattgataat ccaccatgct tcttcgtggc gggtcagagt gagggcaatc
    13381 tttacatcat cattcacctt aaaaacagga atccagggag aggtaacggt gaagataatt
    13441 tgtgttcctt cagtcgcgta aattgtgtta tcggcgatcc cgtgcgcctg tattttttgg
    13501 agtccccacg taatatttgg tttcattgtg
//
LOCUS       DAETPZ010000100        13337 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.100, whole genome shotgun sequence.
ACCESSION   DAETPZ010000100 DAETPZ010000000
VERSION     DAETPZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.100"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..537)
                     /locus_tag="K0W59_003333"
     CDS             complement(<1..537)
                     /locus_tag="K0W59_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3024249.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPAHPSASM"
     gene            545..1055
                     /gene="tnpA"
                     /locus_tag="K0W59_003334"
                     /pseudo
     CDS             545..1055
                     /gene="tnpA"
                     /locus_tag="K0W59_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1073..1741)
                     /locus_tag="K0W59_003335"
     CDS             complement(1073..1741)
                     /locus_tag="K0W59_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HBI3024250.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(2044..2637)
                     /gene="tehB"
                     /locus_tag="K0W59_003336"
     CDS             complement(2044..2637)
                     /gene="tehB"
                     /locus_tag="K0W59_003336"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HBI3024251.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(2634..3626)
                     /gene="tehA"
                     /locus_tag="K0W59_003337"
     CDS             complement(2634..3626)
                     /gene="tehA"
                     /locus_tag="K0W59_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HBI3024252.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            3750..4730
                     /locus_tag="K0W59_003338"
     CDS             3750..4730
                     /locus_tag="K0W59_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HBI3024253.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(4725..5261)
                     /gene="rimL"
                     /locus_tag="K0W59_003339"
     CDS             complement(4725..5261)
                     /gene="rimL"
                     /locus_tag="K0W59_003339"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HBI3024254.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(5324..5548)
                     /locus_tag="K0W59_003340"
     CDS             complement(5324..5548)
                     /locus_tag="K0W59_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HBI3024255.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(5688..7343)
                     /gene="opgD"
                     /locus_tag="K0W59_003341"
                     /gene_synonym="mdoD"
     CDS             complement(5688..7343)
                     /gene="opgD"
                     /locus_tag="K0W59_003341"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HBI3024256.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(7567..8910)
                     /locus_tag="K0W59_003342"
                     /pseudo
     CDS             complement(7567..8910)
                     /locus_tag="K0W59_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            9127..10050
                     /locus_tag="K0W59_003343"
     CDS             9127..10050
                     /locus_tag="K0W59_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3024257.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKESLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(10088..11728)
                     /gene="trg"
                     /locus_tag="K0W59_003344"
     CDS             complement(10088..11728)
                     /gene="trg"
                     /locus_tag="K0W59_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HBI3024258.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQIQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            12104..>13337
                     /locus_tag="K0W59_003345"
     CDS             join(12104..12584,12584..>13337)
                     /locus_tag="K0W59_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HBI3024259.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIDQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFLETRLMY
                     PKKAESLSSSHEKVKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKK
                     RIGEIYHENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGE
                     VGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERP
                     VMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLD
                     NAVVECFFGTLKSECFYLD"
BASE COUNT         3473 a         3291 c         3127 g         3446 t
ORIGIN      
        1 cattgatgct gaagggtgag ccggagtgga tacttcactt tctgtctgaa tactgatgta
       61 ccacgtaccg caggactggc tgacagtgac atttttcaca acacccgtga cctgacggct
      121 attccggtag cgcatccagc ccagtttcgg cagaaaaata cggctgtttt cctgatcgag
      181 cttaacgccc tgcgggtagc ggaatgcatc attctgtccc cgctttttga atcggggaaa
      241 agcagcccgc ttccggaaga agtttttgta agcccgctca aggtctttca gtgactgttg
      301 caatggctgt gagggagaat ctttaagcca ttgcgtttca gtggcatttt tccactcaac
      361 cagccaggaa gccattttcc cgtaagggat gtatttatta ccggcctcat gattctcatt
      421 ctgacgtgcc agagcacgat tgaaaacgaa acgacatgcg cctgcgaagc gcctcatttc
      481 acactcctgt tgaccacctg gtcttaactg gaatttaaat gcctgtaatc gtttcacagg
      541 tcaaatgata ctttggtctg tgaaaaaaga aaccgatatc cggcgtggaa ggcattgtgt
      601 tttcctgatg catgttcacc tggtctttgt caccagatac cgacgccaga tttttgacca
      661 tgacgcgaca gaaaaactac gcacttactt ttcaaatgta tgtgcttatt ttgaagctga
      721 actggttgaa atggatggcg aaccagatca cgtccatttg ttaattaatt aacctcccaa
      781 actggcgata tccagtctgg taaacaaaca gcctcaaagg cgtatcgggt aggttactgc
      841 gacgagatcg accagatatt gcagtcaggt attactacaa aggcgttttg tggagtcctg
      901 gctattttgc cagtagctgc ggaggtgcgc caatatccgt catccgccaa tacattgaac
      961 aacagcaaac acctggtcag gtggaaaacc gcgccttata tccccgccct gaaggatggg
     1021 gttttacggc gcaccggata aaaagcgcca tctaacgatg gcgcgtctgg aactactttt
     1081 tgttaacgtc aaacatggtg gcatcggtcg ccatgtcatc aataacttgt ttaatatttt
     1141 cgaaagccat tggcgtactt tcgttattca ggtctttacc ttcgccctga cgaacgactt
     1201 tgataaccgg tttattagtc gctgcatcaa tcagctcacc ttcaaaatag aggcgagtat
     1261 ccatggtacg gtggcctgta gccatttgcg tccccgcaac cactaatgca acaggaacca
     1321 cttcatagaa ttgcagccct tctttgctgg tatctacacc ggtaatggcc ccacggaaaa
     1381 tcagactacg cggcccagcg gtggtaacca ctggtttacg ctgcgctatc gcttctttca
     1441 tttcggtgtt ggtatagttc aaaattttat ccagaacttt ctgccctacc tgggtagaag
     1501 gttttggtac cggataataa gtgattgggt tccagacgat gctgtcatat ttgctttgat
     1561 caaaactcgg gtctacccaa cgtaaaacag gtttacctgt tgctgaggtt gtttctttta
     1621 aatcagagta attgtttaaa aaaccagaat atttatctgg ctgggtgatt ttagaggcac
     1681 aaccagacag agccaacaag ccgcataaag ctgcaacttt cgcaaatgat gtggtacgca
     1741 tgaatagttt cctgagatga ttatattact ttctaagtta tagcaaaaag cgccgcttcg
     1801 tgttgtggca aaaaaagtgt tggtgaaaat aaatttttca gccccttttc agacgacata
     1861 gtcaatgccg aaaaaggggc taagtcaatc atcagtagtt aaatttcagt aagttaagct
     1921 gctaaaaaac ttcgactact gtgattaatt tcacatccct tcgataaaaa atatgcttac
     1981 cgaaaagaag aacagtaaat catctcagcg tggggaattt tcacgcgttt tgtgcttacc
     2041 gggtcatttt ttacgtgcca gcatcgtggc gaaacgcagt ttaatacgat taccgttggc
     2101 gtcggtgcgg tgcagctcgc cgacgtcttc attgtatttc accctctccc agccttcgta
     2161 atatcgacgt aattctccct ctttgaaggc aaacgtgaag ccgacggtac atggataatc
     2221 agcggtatcc atcgccgcca caatcaagtt gtaaccacca ggtttagtgc aacgttgcat
     2281 attggcaatc aaccctggga tggttttagc ctcaaggaac atcagcacca cagtcgaaag
     2341 aataaaatcg tactgtccat caaatgtgag gttattcaga tcaacgaccc gggtgtgtaa
     2401 attatccaga ttttcaatgg atttaatgcg ctcgacgttg gcgatactca tggcattttt
     2461 atcccatgcg tcaacatcat aaccattggc tgccaggtaa agactgttac gaccattgcc
     2521 acagcccaga tccagcgttt tacccggttt aaccactttc accgcttcca gtacttcaga
     2581 gtgtgtgcgg gttaattcat atttatcagt aaaatagttt tcgtcacgaa tgatcattct
     2641 ttgtcctctg ctttcattaa aacggcgcgc tcggttctga ctaacaattt tccctgcatc
     2701 agaagcgcaa aagtacggat gagcagtatt gcaataataa aattggtaaa gataaacagc
     2761 ggcaccgcca gcgtatggaa aaatccatta tcgctgccac tccccagatg caaaccggtg
     2821 gttgccagtg cagatacgcc gaacgagaaa ctccagaatg aagcattaaa tggctgggag
     2881 agataccatg gcatcagacg tagcataaac agcagttgca gcagtccata accgaaaagc
     2941 attttcgcca gcgtgtcacc ctcgccgccg ttgacgctca gccaggcact acaggccacc
     3001 agcgcaggag cgagctgaat gccgagtgat gtccgcagtg ccgtgggtaa ttctcccgaa
     3061 ctgcgcagac gctgcaagat caccggttca aggcttagcc atgagaaaac gcctgcgcct
     3121 aaaaacacca gaccggcgtc ggtgtagccc aacgcaccac aggccattgc gctgataaag
     3181 ttgttggcaa ctgtcggcag atacagtcca ggcgtggtag cttcctcagg gtgagatccg
     3241 cgccataatc ccgcagtttg ccaggcggca taagccaact gaaccacgac accaaaactg
     3301 aacatgcaca ccgccagtgg gcgaaaccac ggaacaaaac caatcgccac cagcatcgtc
     3361 gttgccggaa acaaactcac aaaactgctc agcactggat ggcgaacttc cgccagcacg
     3421 ctatgcggaa agcgtatgag tcgggtaata aatgcgctag tcaataatcc ccagatgatc
     3481 atcgccagaa tcaccagccc atcccctaac cagtggctga cctgccaaac ctggctggca
     3541 tagcgccagg caaatcccat ccctatcgtc cccaacacaa taccaaagta gcctgccggc
     3601 aaattgagca ctttatcgct ctgcatcttc ttactcattt tgtttaattt ataaagtata
     3661 tttgaaatgc attttatgga attttctcga gcatagccag agccgcagaa tttgctacgg
     3721 ttatagtaag tcacctgtgg gaaaacgcta tgcgcaaata tcgcctaagt gaagaacagc
     3781 gagcctttag ttatcaggaa gatggcacta aaaaaagtgt gttattacgg cagattatcg
     3841 ccattagcga ttttaacgat gtgatagcag gaaccgctgg cggctggatc gatcgcgaaa
     3901 cggtgttagc acaggaagga aattgctgga tttacgacca gaatgctatt gcgttcggcg
     3961 gaacggtgat ttccggtaat acgcgtatca ccggcaccag cgtgctgtgg ggagaggttt
     4021 acgcaacgga taacgtctgg atcgataata gtgaaatcag ccagggtgca tatataagtg
     4081 acagtgtcac cattcatgac tcattagttt acgggcaatg tagaatattt ggtcacgcct
     4141 taattgatca acattctatg atcgtcgccg cacaaggctt aacgcccgac catcaactcc
     4201 tgctccaaat ttatgatcgc gcaagggtta gcgcctcgcg tattgtccac caggcacaaa
     4261 tttatggcga tgccgtcgtc cggtatgctt ttattgaaca tcgcgccgaa gtttttgatt
     4321 ttgccagtgt tgaaggtaat gaagagaaca acgtctggct atgcgattgc gctaaagtct
     4381 acggtcacgc gcaggtgaaa gctggcatag aagaagatgc cattccgacg attcattaca
     4441 gttcgcaggt ggcggaatat gccattgtgg aaggtaattg tgtgttaaaa catcatgtct
     4501 tgattggcgg aaatgccgtg gtacgtggtg gaccgattct gctcgacgag cacgttgtaa
     4561 tacaaggtga gagccgtatt acaggtgcgg tgataattga aaaccatgtg gagttaaccg
     4621 accacgccgt cgttgaagcc tttgatggcg acacggttca tgtccgtggt ccaaaagtca
     4681 ttaacggcga ggagcgcatt acgcgaacgc cgctggcagg gttattatga atcgataata
     4741 cgcgcgtata agttcacatc atcataggca tcattcagga actcagcctg tttcaggcaa
     4801 ccttcaagga taaaaccatt gcgcaaagcg acctggttgc tttgcggatt gtccacccga
     4861 catttgatca cgaagcgtct aagttcacca gactgagcgt aatgatgaat caatgcctgc
     4921 agcgcctgag aaatgatccc ctgcccctga tgagattcgt ccagccagta gccgatttca
     4981 gcagttttat tcagtggttc aatacgatta aacgagataa cgccgataag ttcatcttct
     5041 ttgaaaatca tgaacatttt ggcatagccg cgttgatgca acatcacatt accctgcacc
     5101 gtttttcgcg tgtcctcttc actctgaacg aactgcggcc agtttagcga ctgctgtaac
     5161 caggttttat ttttacagat taactgataa agaggtgtga cgtgactttc cgcaacagca
     5221 tgtaattcaa gtgattcgct tacttttatc gtttcagtca tcttatctcc agtcaatcca
     5281 tgctgttgtt aaaaaagccg cctggctggc ggcttaggaa agtttacacc tctttgacgc
     5341 tctcctgctg caggattttg agcatctcat cttttaactg tagcttttgt tttttcatgc
     5401 ggaccacttc cgcattgtac cctcgaccgt cggaaccttc ctttctggca atttcatgat
     5461 caagtttatt gtgtttatcg aacaaggaca taaagcgagg attttcgttt ttcagacggg
     5521 atattaaatc tcggtattct ggaaacatac gcactccctg ttagtggctg acacggtgta
     5581 tctgaaatac acaccatcag attactcatt taacttaggg aaaaatgcga gcgggatcgt
     5641 gtttaaccag acggcacctc ccgaagaagg tgccgaagaa aaaacgatta actcatcacg
     5701 cggtcgtcaa catactgacg tttatccggc gctggcggga aatactgata cagccatgtt
     5761 tcactgatag cgtccccctg acaacgtaga tacatccgca tatcgaccgg atcagtggag
     5821 tccgaagtcg gataccagtc aaactgaata cgataaccat cgatgggttc aatatagaga
     5881 atttcgattt gcttcgcttc cccactggaa agcgtaatca ccggctcaat gccttttggc
     5941 gcggcagctt tcagatcacc accaacgaaa tcgacggcaa aacgacgcgc ccatttttcg
     6001 ggatagtgtt cacctggcgc ccaaccttcc gggaaaccgc ccatgccggt acgcgtcgcc
     6061 ataacgcgcg ctaatgggca atgaacaggc ggttgcgcac tccagtacag acgatactgg
     6121 aatgcaaact catcacctgc ttttacagct ttttctggct gccagaagca gacaatgtta
     6181 tccagcgttt cgcccgttgt tgggatttcc atcaggccga tggtgccctt accccacttg
     6241 ttacgcggtt ccacccacag gcttggacgt ttgttatacc agcccataat gtcctgataa
     6301 tgggagaagt cacgatccag ttgcaataaa ccaaaccctt tcgggttgtt gtcggtgtaa
     6361 gcattgaact gcaatttttg cggattattc agcggacggc aaatccactc gccgttgccc
     6421 cgccacatgg acagacgatc agagtcatga atttgcggat gaattgtatc gcacatccga
     6481 cgttcattag taccgcagct gaacatactg gtcatcggcg aaatgcccag ctgtttaatg
     6541 tctttgcgcg catacaggtg attttccaca tccataatca cctgactttt ctcacaatgg
     6601 atagtgaact tataggcacc agtaatgctg gcgctatcga gcaacgcata aacggtaaag
     6661 gtagttgccc ccggttttac cgtatcaaac cagaaggcgg taaagtcggg gaactcttct
     6721 ttactgtcgg tgtaagtgtc gatcgccagg ccgcgggccg acaaaccgta ttgatatgta
     6781 tcatcaacgg cgcggaaata actcgcgccg agaaatgata ctacatcacg gcgcgccagt
     6841 tcgggggctt taaacacgcg aaaaccggca aagccgagat cgctttgccc ttctaattgt
     6901 ttggtatcaa cacctgcatc gttgtatttg aacaactccg ggcgaaagtg aatttcacgc
     6961 gccagatgtg ttgctggatc tacagaaaac atacgaacgc ggcgacggaa tcccattccc
     7021 atatggaaga actgagcgtc cagttgacgg ttctcaacgt tatgccagag cgatttttcg
     7081 gcgtcgtatt gaatactgtt ataagcctgc ggcgtcattg tcgccagcgt gtcaggtaac
     7141 ggacgaggcg caccacgcca cgctgtttgc gctaagtcgt gcgccattga ctgtagaatg
     7201 gagaagtcaa aacgctgggt ttgcccgtcg gcaatatcag aatctgccgc gaatgctgcc
     7261 tgagaaaaaa gagaagcaat gccgctggta ccgcacacgg cggccatagc cattgaacct
     7321 ttaataaatc gtctacgatc catacctgaa agtgagtcct tctggtgttg tgaatggtcc
     7381 cgcgctggtc gttttcagcg gcaagaaagt gcttatttat gaccgctcac tctagacaat
     7441 atttattaat aatccaattg ttgaactgag agaatatgca taaatcggca tttttttatg
     7501 tcgatgggat gagcctgatt cgtttgtaaa atcaggcaaa tttttgcaaa ggcggatggt
     7561 cagaatttaa agcaaaccac agcgacgttt actgcgttct tccgcttctt ggtaaagctg
     7621 gaactcatcc aaaaccggac aacccaacgc ctgctcaaat gcttcgcgac tggcattacc
     7681 gtgactgcgt gtctgcgttt cattacccag atttgactgg aagatccccg ccgcgctaac
     7741 gggcaagaaa tcttcatagg tgatgggttg cgccactacc caaccacgtt caattaaggg
     7801 ctgtggatcg tctccaggat gaatcgcctg acgatgcgcc tcacccgaag gcgtcagacg
     7861 gtaccggaac catgccaacc cttgctgacg cattaaaaac tcactgtcag gaaaagtgcg
     7921 gaaggtttcc tgtaaatgca tttggtgagt gagattatcc tgcccggttc cagcgttacg
     7981 cagaagatca tcatacagtt gtcgcccttt cggcgttaat gccacgccac gctgctcaat
     8041 ttcaccaaag cgcgcggtat gcgtgccctg tttctgcccc gcaaacaaca ccgtctcttc
     8101 cagtgcttta aagctggtct ggcgtagtaa aatcggtacc tcgcggcgcg gcggcccctc
     8161 gatgagaatt ttgggttcaa ttccgcattc aggcatcatc gactgcaccc ggtcaatatc
     8221 cagcgtacgt ggcgtcaggt ggttgatatg gcatccagga aaacagacca catcagcaat
     8281 caaccgatgt tcgttgtgca aggcgcgata ggtttcttca tctaccgttg ctaactagtg
     8341 ccagcgaaac gtttccaggg cttcctgcac aaactcctgt gcctgtgttt cgttaaaacc
     8401 gccctgctgc tcatattcct ctaacagttg tcgacaacgt ggggtgaaga tatcgcgctg
     8461 acgcagaatc tccgccgctt tctggcgcaa aatttcgttc tcgataagct caaggcggag
     8521 taaggatgta aaaacgcgga agggattacg cgccagagaa gcatcatcaa tgggccgaaa
     8581 tgctgtcgaa tgtaccggca cccctgcctg cgagagatcg taatagctca ccgggtacat
     8641 ccccataatg gcaaacatcc gccgaagagt agcaagctct tgtgcagtcc caacgcgaat
     8701 cgccccatga cgttcaacat tcagtcgcgc cagctcgtct gcatttacca ttttttcgtg
     8761 cagttgagga ttgttttcca gcacagccag attcacatca gctaccagtt ccagcagcgt
     8821 gccatattgc ggaacttctt gctggtacat ggctgacatt gcctgcgaaa actgttcccg
     8881 aatctcatcc gccgtgatgc tgttcgccat agtgtctgcc tccagtggtc agtaatctgg
     8941 agtgtaggta acagcattca ctctggcggg aagaatttac aaactgtgat ctcgccgcga
     9001 aaacatcaat attatccatt ttgctgtaac ataattgctt taattgttaa taatatttta
     9061 caatcaagtt atcataatca aacaacttca cttgtcagcg acaccgcttc gtttttaaca
     9121 tcgcttatgg aaaaaaatag tctgtttagt cagcgcatcc gtttgcgcca ccttcataca
     9181 ttcgtagctg tcgcacaaca aggaactttg gggcgcgcag ctgaaaccct taatttgagt
     9241 caacctgcgc tctctaatac attgaatgaa ctggagcagc tgacgggcgc tcgcttgttt
     9301 gagcgtggtc gtcagggggc gcaacttacc ttacccggcg aacaattttt aacgcatgca
     9361 gtcagagttc ttgacgccat caacactgcc ggacagtcgc ttcatcgtaa agaaagtctt
     9421 aataatgatg tcgtcagggt tggtgcacta cctactgcgg cactggggat attaccttcg
     9481 gttataggtc agtttcatca gcaacaaaaa gagacgacct tgcaagttgc gacaatgagt
     9541 aaccctatga ttctggcggg tttaaaaacc ggggaaatcg atatcggcat tggtcggatg
     9601 tcagatcctg aactgatgac cgggcttaat tacgaactgc tgtttcttga atcgttgaag
     9661 ctggttgtcc gccctaatca cccgctactt caggagaacg taacgctaag ccgggtgctg
     9721 gaatggccgg tcgttgtatc accagaaggc actgcgccac gccagcattc agatgcatta
     9781 gtgcagagcc agggctgtaa aattccttcg ggttgtatcg aaacgctgtc tgcttcgcta
     9841 tctcgtcaac ttaccgttga atacgattac gtgtggtttg tcccttctgg cgctgtaaaa
     9901 gacgacctgc gtcatgccac gctggtggcc ctgcctgttc cgggacatgg tgcaggcgaa
     9961 ccgattggaa tactgacccg cgtagatgcg acgttctctt ctggttgcca gttgatgatt
    10021 aacgctattc gaaaatcaat gccgttctga aaggtgaagg gatctgtcga tccctccttg
    10081 aacattttca caccgtagcg aaactaactg gttcacccgc tccgcgaggt tctgccgaca
    10141 cagaatgttt gttcagacgg aatacgttca ccgcctcagt taatcgtgca gcctgttctt
    10201 caagggacac cgccgctgct gaggcctctt ctaccagaga agcattctgt tgcgtcacct
    10261 tatccatttc agaaatcgcc tggctaacct gagttatgcc tctactttgt tcatccgagg
    10321 cggcggcgat ttcctgcatg atatgtgtga cactcgcgac ggcatcaaca atagtgctca
    10381 tggttttccc tgccgttgcc acctcatccg accccaggtc aattaacctg actgattcac
    10441 tgatcaagcc ttcaatctct ttcgctgcct gagcgctgcg gcttgcgagt gtccgtactt
    10501 cgctggcgac aacggcaaat ccacgccctt gctcacccgc tcgcgcggct tcaacggcag
    10561 cattcagtgc cagaatattc gtctggaaag caatactgtt gatgacggcg gtgatctcag
    10621 aaattttctt cgaactcgtg gagatagcgc ccatcgtttt tactacaccg gaaaccgtct
    10681 gcccgccatc gctggcttta atagaagcct cttgcgccag tttgctggca tgatgcgcgt
    10741 tatccgcatt ctgtttcacc gtcgcagtga gttgctccat gctggcggca gtttgttcga
    10801 tagccgccgc ttgttcttcg gtgcgagatg acaggtccgc attgccagct gaaatttcgc
    10861 tggtgccacg ataaatctct tccgcgccct gtcgaacagt ccctactgtc atccccagtg
    10921 aatgctgtat ttgctgtaaa tgacgactta agcgaccgat ttcattacgg cccgccggtt
    10981 catcattcat cgtcagatcg ccactggcga ttttttcaat ccgttgtgcg gcatgttgca
    11041 gtggacgaat gacgatccga cgtagcacca taaatgttat cagcgtcatg accagggcaa
    11101 gcacaaacgc gccaatcatg aacatcccac ccaggcgggt gcgctgatgg gccagttccg
    11161 ccagttggtt ggctctggtg ctacgtatct taacggcttt gttcaaaata tcggtataag
    11221 cattatccag cggtcggatc tgctcacttt catgattgat aatcgcttca aacatgccat
    11281 ttttggcata tttcaacata ggttgcatac ccgtgatata agcctgaaag cgttgattta
    11341 attcagtgtc gagggcttca tcagcaggtg ttttcaccgg tcgattctga taagcacgat
    11401 aaccttgctg cgactgttta atctccgatt cggcttgcgc aatatttcgc ttcattgctt
    11461 ccatttccgc aatacgactc gcagccccgg cctgaatcat attgatccgg gctgaacgca
    11521 aaaaatctga actgttcgct aacccggtcc gaatctcaat ctccgcagta acatcacgct
    11581 gatcgcgatc ggcctgccag aggaaatagc cagccagagc agaacttaat gcgaatagaa
    11641 ctaagatacc gccaagaatg caggcaaata acggaaccag cctgatgtga tgcaaaaaac
    11701 ctaatcgctg tgagggagtt gtattcatta ccgttgtctc tcgtccaggt ttactcctga
    11761 tagaaagtca tcggcacttg acggtaatta cttatgacca aaaacctctt tgcgtcacat
    11821 ttttacaaca attattgcag agaaaattaa tgctattgga atgaagagag taacgaatgg
    11881 ctaaactggc taacccgaat gacgaaaaat tctggaaaac agcacgcaat ttcgtgaagt
    11941 gattattttc gtccctaagg aaacctcata ggatgcctgc gggtgcctga gactttctgt
    12001 tttcaggcta accgggaatc agaaagacga aggccccgac cgatatttct atcaactgta
    12061 ctgcacccat tttgttggac gatgaaatgg aatagcccct aatatgtcaa agccaaaata
    12121 cccttttgaa aagcgccttg aagtcgtgaa tcactacttc actactgatg atggttacag
    12181 gatcatctcg gcacgttttg gtgtcccccg aacccaggtc aggacatggg ttgccctcta
    12241 tgaaaaacat ggagaaaaag gtttaattcc caaacctaaa ggcgttagtg ctgatccaga
    12301 gttgcgtatt aaggtcgtga aagctgtgat cgaccagcac atgtccctta atcaggctgc
    12361 tgctcacttt atgcttgctg gtagtggttc tgtagccagg tggctgaagg tctatgaaga
    12421 gcgcggagaa gctggtttac gcgcgctcaa gattggcacc aaaagaaaca ttgcaatatc
    12481 agttgatcca gaaaaagcgg catcagcatt ggagctgtca aaagaccgac gcattgagga
    12541 tcttgaaagg caagttcgat ttcttgaaac gcggcttatg tatctaaaaa agctgaaagc
    12601 cttagctcat cccacgaaaa agtgaaagta ctcaacgagc taaggcagtt ttatcctctt
    12661 gatgagcttc tcagggctgc ggagataccg cgcagtacgt tttattacca tctaaaggct
    12721 ctcagcaagc ctgacaagta tgcggacgtt aaaaagcgta ttggtgagat ttatcacgag
    12781 aatagaggcc gatacggata ccgtagggta acgctgtctc ttcatcgaga agggaaacag
    12841 attaaccata aagctgttca gcgcctgatg ggaaccctct cacttaaagc agcgattaag
    12901 gtcaagcgat accgctctta cagaggagag gtagggcaaa ccgcccctaa tgttctccaa
    12961 agagatttca aggctacgcg gccaaacgag aagtgggtta ccgatgttac tgaatttgca
    13021 gtcaatgggc gcaagctgta tttgtctcca gtaatagatc tcttcaacaa cgaagttatt
    13081 tcttacagcc tttcggaaag accagtgatg aacatggttg agaatatgct cgatcaggca
    13141 ttcaaaaagc ttaatcctca cgagcatcct gttctgcact ctgaccaggg atggcagtat
    13201 cgtatgagaa gatatcaaaa tatccttaaa gaacatggta ttaaacaaag catgtccaga
    13261 aaaggcaatt gtctggataa tgctgtggtg gagtgtttct ttggaacctt aaagtcggag
    13321 tgtttttatc ttgatga
//
LOCUS       DAETPZ010000101        13176 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.101, whole genome shotgun sequence.
ACCESSION   DAETPZ010000101 DAETPZ010000000
VERSION     DAETPZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.101"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            19..255
                     /locus_tag="K0W59_003346"
     CDS             19..255
                     /locus_tag="K0W59_003346"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024260.1"
                     /translation="METHALSDDCLDCVYDWYAEKNGQFFSATSPEELLGLVAMWEVR
                     GNDWRANLNEINTYQKAIEESPFFDKNGNEIESD"
     gene            complement(321..553)
                     /locus_tag="K0W59_003347"
                     /pseudo
     CDS             complement(321..553)
                     /locus_tag="K0W59_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(663..1433)
                     /gene="yafV"
                     /locus_tag="K0W59_003348"
     CDS             complement(663..1433)
                     /gene="yafV"
                     /locus_tag="K0W59_003348"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HBI3024261.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            1614..2060
                     /gene="ivy"
                     /locus_tag="K0W59_003349"
     CDS             1614..2060
                     /gene="ivy"
                     /locus_tag="K0W59_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HBI3024262.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(2103..4547)
                     /gene="fadE"
                     /locus_tag="K0W59_003350"
     CDS             complement(2103..4547)
                     /gene="fadE"
                     /locus_tag="K0W59_003350"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HBI3024263.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADSIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            4786..5364
                     /gene="lpcA"
                     /locus_tag="K0W59_003351"
     CDS             4786..5364
                     /gene="lpcA"
                     /locus_tag="K0W59_003351"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HBI3024264.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5570..6337
                     /locus_tag="K0W59_003352"
     CDS             5570..6337
                     /locus_tag="K0W59_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HBI3024265.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(6308..7048)
                     /locus_tag="K0W59_003353"
     CDS             complement(6308..7048)
                     /locus_tag="K0W59_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HBI3024266.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            7432..7953
                     /locus_tag="K0W59_003354"
                     /pseudo
     CDS             7432..7953
                     /locus_tag="K0W59_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            8129..8626
                     /gene="rayT"
                     /locus_tag="K0W59_003355"
     CDS             8129..8626
                     /gene="rayT"
                     /locus_tag="K0W59_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HBI3024267.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(8843..10555)
                     /locus_tag="K0W59_003356"
                     /pseudo
     CDS             complement(8843..10555)
                     /locus_tag="K0W59_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            10527..11312
                     /gene="lafU"
                     /locus_tag="K0W59_003357"
     CDS             10527..11312
                     /gene="lafU"
                     /locus_tag="K0W59_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HBI3024268.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            11383..12438
                     /gene="dinB"
                     /locus_tag="K0W59_003358"
     CDS             11383..12438
                     /gene="dinB"
                     /locus_tag="K0W59_003358"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBI3024269.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            12490..12783
                     /gene="yafN"
                     /locus_tag="K0W59_003359"
     CDS             12490..12783
                     /gene="yafN"
                     /locus_tag="K0W59_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HBI3024270.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            12786..>13176
                     /gene="yafO"
                     /locus_tag="K0W59_003360"
     CDS             12786..>13176
                     /gene="yafO"
                     /locus_tag="K0W59_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HBI3024271.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRM"
BASE COUNT         3186 a         3452 c         3398 g         3140 t
ORIGIN      
        1 aagggatata aagtaacaat ggaaactcat gcattatctg acgattgttt agattgtgta
       61 tatgattggt atgcggaaaa aaatggacaa ttttttagtg caacgagtcc agaagagtta
      121 cttggattag ttgcaatgtg ggaagttcgt ggtaatgact ggagagctaa tttgaatgag
      181 ataaatactt atcaaaaagc catagaagaa tccccgtttt ttgataaaaa cggcaatgaa
      241 atagagtctg actaaatttt attgatatag caacgataat aaccggaaat ttcttccaga
      301 tctttccggc tttttattat ttaactttcg ttcagcacac taaacatccc gtctcgcatc
      361 cccttaactg actgtttaac ctgataaagt tccctgcgca gttcctgtac ttcgttgtta
      421 tcccaagcag gatcaaacat ccctccgaga tcttcacggt aacgcccgtg tcgatctcaa
      481 atcccgcttt tctcagccat ttggcgctga ttttgatcac cggcgtgtcg ctgcggttgg
      541 gcagataacc cacggtacag cgacgctgtg ttttggaaat ggtgacttct ggcgtaatat
      601 tgtgtttaag ttaggggctt tcgtaatcta gcccagcccc cggccctaaa cacaaccccc
      661 actcaccaca acctaaactc atccgcatcc cgccatgccg gaaacttttc tctatattcc
      721 cgcaatgcca ccatcgacag ctccgcatca atacgcgttg ccttatgcgc gtcggcggta
      781 gcgataatct cgccttgcgg attaatcacc cggctgtcac cgcgataatg gcagccgttg
      841 ccatcgctgc cgacgcgatt gcatcccgcc acatacgcct gattctcaat cgcgcgggcc
      901 gtcagcaatg cctgccagtg cagagagcgc ggagctggcc agttggcgac gtacagggcg
      961 aggtcataat cgttgagatt gcgcgaccac actggaaaac gtaagtcgta gcacaccagc
     1021 ggcaaaatac gccagccgcg ccattccaca atcactcgcg cattgcccgc tttataatgc
     1081 agatgctcat ctgccatgcg gaacagatga cgcttatcat aaaaatgtac cgtgccgccc
     1141 ggctcaacca gcaaaaagcg gttaaccgaa ccagactccg tttgtaatgc aacactgcct
     1201 gcaatcagcg cattgcactg ctgcgcctta gctgtcatcc agttcactac gtcatcttgt
     1261 gcaagcgacg aagctgccgc ttccatggca aagccgctgg taaacatctc cggtagaacg
     1321 atcacatcgc gcccggtaat accttccagt tgacgatcaa aatggcgcag gttggcagga
     1381 ccatccaccc acaccaacgg ttgctgcaaa agcgtaatct tcaaactagg cacggtgtac
     1441 aactccttta tgcgaagggt tttataactt taacacctta tcaggcagtt gccttagcgc
     1501 agaataaatt gataacaaat gctgatattg gaaatatctg atttgcaaat tatcgtgtta
     1561 tcgccaggct ttaggaggtt aataacatgg gcaggataag ctcgggagga atgatgttta
     1621 aggcaataac gacagtcgcc gcactggtca tcgccaccag tgcaatggcg caggatgatt
     1681 taaccattag cagccttgca aagggcgaaa ccaccaaagc tgcgtttaat cagatggtgc
     1741 aagggcataa gctacctgca tgggtgatga aaggcggtac ttatactccc gcacaaaccg
     1801 taacgttggg agatgagacg tatcaggtga tgagcgcgtg caaaccgcat gactgtggct
     1861 cgcaacgtat cgctgtgatg tggtccgaga aatctaatca gatgacgggg ctgttctcgg
     1921 ctattgatga gaaaacgtcg caagagaaac tcacctggct gaatgtgaac gatgcgcttt
     1981 cgattgatgg taaaacggtg ctgttcgcgg cgttgaccgg cagcctggaa aaccatccgg
     2041 atggctttaa ttttaaataa ttagcggata aagaaacgga gcctttcggc tccgttattc
     2101 aattacgcgg cttcaacttt ccgcactttc tccggcaact ttaccggctt cgtcgccagc
     2161 tcttccggat caaagtcatc aacgttaata ctgcgcagac ggctttcttc cgctttcacc
     2221 agaatagcgg cttcatcttt atcaatcagc cccttcgtca gcgcgttgtg cgccagttca
     2281 tccagacggg taaatggcag atttttaccc agctctttac agatccgctg atgaatcgag
     2341 tcggcggcaa tcacatccac caacgcctct tccagtaaac caaccgggtt atgctcgctt
     2401 ggcgtcaggt actgaccgcg accaatacgt gaacgagtgg cgttcggcac ttgtaaaatc
     2461 ttcgccactt tatgatccag cttgtcagac ggtgccagat aatgacgtcc ggtcgggaag
     2521 atcaccacat tcagcagccc ggcaaccacg cggttcggga agttttgcag taaatcgtcc
     2581 atcgcctgtt ctgcctgata cagcgcatcc tgtacgcccc agtgcaccag cggcagatcg
     2641 gcttcattac ggccttcgtc gtcataacgc ttcagaacgg cagaggcgag gtaaagctgg
     2701 cttaaaatat cccccagacg tgccgagata cgctcacgac gtttcaggct gccgcccagc
     2761 agtgccatcg agacatcaga aagcagggcg aggttggcgc tcaggcggtt caggtgctga
     2821 tagtagcgtt tagtggcatc gccggttggc gtgctgctgg ttaaaccgcg cgtcaggccc
     2881 agccagaagc tgcgaacttt gttgctaccg acgtgaccga tatgtttgaa caacagttta
     2941 tcgaacgcgt tgacgtcatt gttcttcgcc gcttccatct cttccagcac gtacggatgg
     3001 caacgaatcg ctccttgtcc gaagatcatc atgctgcggg tcagaatgtt agccccttca
     3061 acggtgatgg caatcggtgc gccctggtaa gcacgcgcca ggaagttgct ttgcccgagc
     3121 ataatgcctt taccgccggt aatatccatc gcatcaataa tcgactgctg cccgcggtgg
     3181 gtacagtgat acttaacgat agccgacagc acggcaggtt tttcgccgag cataatgccg
     3241 taggtaatca gcgatgccgc agcatccatt acataagcgt tcccggcaat acgcgccagc
     3301 ggctcttcaa tcccttccat cttaccgata gagattttga actgacggcg aatgtgagaa
     3361 tacgcgcccg ttgccagcgc taccgatttc acgccgccgg ttgagttgga aggcagggtg
     3421 atgccgcggc ctaccgagag gcactccacc agcatccgcc agccttgccc ggccattttc
     3481 ggcccgccga ttatgtaatc gatcggcacg aagacatctt taccgcgcgt cggtccgttc
     3541 tggaacggta cgttcagcgg gaagtggcga cgaccaattt ccacgcccgg cgtggtggtt
     3601 gggatcagcg cacaggtaat gcctaaatct tctgcaccgc cgagtaattt ttccgggtcg
     3661 gagagtttaa acgccagccc aagcacggtc gcaatcggtg ccagcgtaat gtagcgtttg
     3721 ttccaggtca gacgcatccc cagcacctgc tggccctgcc attcgcccat gcagacaatc
     3781 ccggtgtccg gaatcgcgcc cgcatcggaa cccgcttccg ggctggtcag tgcaaagcag
     3841 gggatctcct gaccacgcgc cagacgcggc agatagtgat ctttctgctc gtcagtgccg
     3901 taatgttgca acagttcgcc cgggcctaat gagtttggca cgccgacggt aatcgccagg
     3961 atcccgctca cgccagagag tttttgcagc acacgagact gggcataagc tgagaactcc
     4021 agtccgccgt actctttttt gataatcatc gcgaagaagc gatgctcttt aaggtacgcc
     4081 cacaactccg gcggcagatc cgccagctca tgagtaatct ggaaatcatt cgccatccgg
     4141 caggcttctt ctaccgggcc gtcgagaaac gcttgctctt cagcggtcag gcgcggctgc
     4201 ggatagttat gcagcttttt ccagtccggc ttgccctgga acaagtcgcc ctcccaccag
     4261 gtggtgcccg catcaatcgc ttctttctca gtgcgcgaca tcggcggcat caccttacgg
     4321 aaaccgcgaa ataccggcgc ggaaatcatc gacttacgca taggcgcaaa gttaaatggc
     4381 acgaggataa tggtcagagg caccagtacc cacgccgacc acagaccagc aacgccgagg
     4441 gcggctgtcc aggcgagcaa aatcagactg ctgataaata agctcacgcg gtgatagaac
     4501 aacgcgccga gcaggacaac cgtagcgaga atactcaaaa tcatcataac gaaaagcccc
     4561 ttacttgtag gaggtctgac cactgtgatg atatggttgt agtggatgta aaaacattta
     4621 gcaatgtgtt tacaatataa ttacaacaaa gctcacattg ttgctgtttt tatccgcact
     4681 tcaggtcaaa aagtcctggt catagcacct gcccgtactt ctcgcttttg gcggtatccg
     4741 gtacactgca ttttgtctat tacatttatg ctgaaggata tcctcatgta ccaggatctt
     4801 attcgtaacg aactgaacga agcggcggaa acgctggcta actttttaaa agatgacgcc
     4861 aatattcacg ccattcagcg cgcggcggtc ctgttagcag acagctttaa agccggtggc
     4921 aaagtgcttt cctgcggcaa cggcggttcc cattgcgacg ctatgcactt tgccgaagag
     4981 ttgaccggtc gctaccgtga aaaccgtccg ggctacccgg cgattgctat ttctgacgtt
     5041 agtcatattt cctgcgtcgg taatgatttc ggtttcaatg atattttctc ccgctacgtt
     5101 gaagcggtag gtcgcgaagg cgatgtactg ctggggatct ccacctccgg taactctgca
     5161 aacgtgatca aagcgatcgc agcggcgcgt gagaagggga tgaaagtgat caccctgacc
     5221 ggtaaagacg gcggtaaaat ggctggcacg gcggatatcg aaattcgcgt tccgcacttt
     5281 ggttatgccg accgcattca ggagattcac attaaagtga tccatatcct gatccagtta
     5341 attgaaaaag agatggttaa gtaagtctgg cgtaggccgg ataaggcgtt tacgccgcat
     5401 ccggcacttg tgcgctgatg cctgatgcga tgctgacgcg tcttatcatg cctacaaatc
     5461 tgtacccgaa ccgtaggccg aataattcgt ttacgccgca tccgacctga aaattcttaa
     5521 atcaatcttc gccggggtcc atgcgctccc gctgttgtgg aggttaccca tgtgcgaatt
     5581 gctcgggatg agcgccaacg tccctaccga tatctgcttt agtttcaccg gacttgtaca
     5641 gcgtggtggt ggaaccgggc cacataaaga tggctggggc attacctttt acgaaggtaa
     5701 aggctgtcgc acatttaaag atccacaacc cagctttaat tcccccatcg ccaaacttgt
     5761 ccaggactac ccgataaaat cctgttcggt ggtggctcat attcgccagg ctaatcgggg
     5821 cgaggtggcg ctggaaaata ctcacccatt tacccgcgag ttatgggggc gtaactggac
     5881 ttatgcccat aacggacaac tgacgggcta caaatcactg gaaaccggca acttccgccc
     5941 ggtcggtgaa accgacagcg aaaaagcctt ttgctggctg ctgcataaat taacgcagcg
     6001 ttacccgcgc acgccgggca acatggcggc agtgtttaaa tatatcgcct cactggcgga
     6061 tgaactgcgg gcgaagggcg ttttcaacat gctgctttcg gacgggcgct atgtaatggc
     6121 gtattgctcg actaatttac actggatcac ccgccgcgcg ccgtttggcg tggcaacgtt
     6181 gctggatcag gatgtggaaa tcgacttcag ctcgcagacc acaccgaatg atgtggtcac
     6241 ggtgattgcg acacagccgc tgacgggcaa tgaaacctgg caaaaaatta tgccaggcga
     6301 atggcgctta ttttgcctcg gggagcgtgt agtttgatgc cagttgtggc tgcaccactt
     6361 cgtggcttaa cggcttgctg accacgtaac ggccattggc cacagaaacg gtcggtggct
     6421 tacgggtttg ctcaaagtag tcgtagcccg gcttcagctg ctcccagaag tccttaaagt
     6481 tggaatattt atggcgcttc atattggcgt cggtcatgcg gaatgggtaa atactcacct
     6541 gcacgctcgg ctgaccaaac accagcgcac cagtaacgaa ctggaagatc tcatcaatac
     6601 cctgattggt cattgcgtag cagccgatgg atacacaatc gccgtggatc atcaggtatt
     6661 tcccttcata accatgcgca cggtcataag cattggggaa tccaatatta atcgctttgt
     6721 agtaacggct gtctggtttt aactgattac gctggacgct ataaaatcct tccgggcttt
     6781 tgaaatcgcc ctgacgctgt tttggcccta agccgccgga atatttacag attttataac
     6841 tgtcgagcag ttgatattgc tcgcccattt tgacgtaaag atcgagcgta cgttcttcct
     6901 tgaagatctg gatgtagaca ggggatccca tcaactgctg cttatactct ttgctcactg
     6961 gcgtggtcga gctactgctg cccagcaaac cggcaaacga aacgcacggg atcaacaaca
     7021 tcgcaagaat taatgcgatt ttacgcatac tgcttattcc ttgataaaac ggttacacac
     7081 gccaggacgg caaaatggat cccaaatcgg aatagtctgg atttggaagg ctcacattat
     7141 caccaaaaga gttttacgca agcctgtcgg cgctgggttt acaaatttag cacaggggca
     7201 gttttcaata gtccgaaaag cgcatgtatc atttatttcc taatattatg aaatctcaat
     7261 agtaatatgc aaataagcaa ttaataatcc cgtttactcc tccggtcttt taaaattctc
     7321 accttcattc agccggttta tgtctttcat gtcttcgttc ttgctcggca gatttttgca
     7381 tccaggcgtg ttttcgttat gcgttttgct ccctttactc gccagcgcca ccacaaccgg
     7441 gagcagataa aacagcatcc agaatggttt cctgcgccgc tcaaggcgag tgacagacgc
     7501 tggcaggagc tggcggaaaa caaccacttt ttaagcagcg atcacctgca taacatcacc
     7561 gaagtggcga ttcaccgcct ggagcagcag cttggcaagc cttacgtctg gggcggcacg
     7621 cggcccgatc aaggcttcga ttgtagtggg ttagtttttt atgcctacaa caagatcctt
     7681 gaggcgaaac tcccgcgcac ggccaatgag atgtaccact accgtcgggc aacgattgtg
     7741 gcgaataacg acctgcgccg gggagatttg ctatttttcc atatccacag ccgcgagatt
     7801 gccgaccata tgggggtgta tctgggcgac ggtcagttta ttgagtcact gcgcaccggg
     7861 gaaaccattc gggtaagccg gttagccgaa cctttctggc aggaccattt tttaggcgca
     7921 cgcaggattt tgacggaaga gatgattttg taggccggat aaggcgttta cgccgcatcc
     7981 ggcggttgtg cacggatgcc tgatgcgacg ctgacgcgtc ttatcatgcc tacaaatctg
     8041 tgcgtttttg cgagccgctt tcccgatata acacagcgtc attgtcggcc tttccttttc
     8101 ctcttactgt tgagaaaaag gagtcgctat gtcggaatat cgtcgttatt acatcaaggg
     8161 gggaacatgg tttttcacgg taaatttacg caatcgtcga agccatcttc tgaccaccca
     8221 gttccagacg ctccgtaacg ccattattaa cgttaagcga gacagacctt ttgaaattaa
     8281 cgcctgggtc gttttgccgg agcatatgca ttgcatatgg acattacccg aaagcgatga
     8341 tgatttttcc tctcgctggc gggagattaa aaagcagttc acccatgctt gcggattgaa
     8401 aaatatctgg caaccgcgtt tttgggagca tgccattcgc aatacaaaag attatcgcca
     8461 tcatgttgat tatatttata taaatccaga gaaacatggc tgggtaaagc aggtgagtga
     8521 ttggccattc tcaacgttcc atcgcgatgt cgcgcgaggg ttatatccca tcgattgggc
     8581 gggagacata acggatttaa gtgccgggga gcgtatcatt ttataactgt gcgctggtgc
     8641 cggatgcgct tacgcttatc cggcctacaa atcgtgcttt aacggtaggc cggataaggc
     8701 gttcacgccg catccggcag ttatgcgcag atgcctgatg cgacgctggc gcgtcttatc
     8761 atgcctaaaa acttgtgcct gaacggtagg ccggataagg cgtttacgcc gcatccggca
     8821 attgtgcacc gatgccggat tcttacatca acgcccccat aatcttcaac tccagctcat
     8881 ccggcacttc gttatacgac agcacatgca gccccggcgc aaacaacctt gcataacgcg
     8941 ccagcaaagg gcgtagctgc ggcggcacca gcagcaccgg atctttcccc gccgctttca
     9001 tctgctcttt cacctgtggc atcgtgctct ggaactggtt gagcatattc gggtcaaccg
     9061 gcacgctgtc gagcatcact ttgccggcct gttgcgcctg attcactacg ttggtcagca
     9121 gattttccag ctcattattc agcgtataca ccgtcagctc ctgcttgcga acgaacggat
     9181 gagtaatgct gcgccgcagc gccaggcgca catcggccgc cagcagaata tgatctttag
     9241 tcaccgtgct actggcgacc agcacggtgg cgatggtgac gatatcgcgc agggaaacgc
     9301 cttcggtcag cagcgcccgg tacactttca gcaattggct gtaattgagc gccgcgctta
     9361 aatcctccgc caggcgcggt gccatcgacg acaaacggtt atgcaactgc gtgatgtcat
     9421 catagttaaa caaatcagga atatagctgc gcacaatcgt gttcacatgg gtggcaatca
     9481 cactggcgct gtcgatcacc tgatacccca tattcagagc cttcgctttc tgcgcaggct
     9541 ggatccaggt gaccggtatc ccgtacgccg ggtcgttccc cagcacgccg tcaatctcgc
     9601 cgtaggtttc gctggagggc agcgccatca gtttatccgc cggaatatcc gcttcatcag
     9661 ccttaatgcc gttgatgaaa atagcgtact ggctgggctt gaggcggaag ttttcccgaa
     9721 tgcggatctc cggcagcagc acgccgttac cgtcggaaat cacctgccga acgccgcgaa
     9781 tccgctgggt gagcgggttg ccctgcgctt tgtccaccag cgccaccagc ttgtagccga
     9841 ggcttaagct gatgggctcg atcagcggaa tagtttccca actgacctgt tgttcgctgg
     9901 tttcagtgat ggtgcgggtc agcgtttcga ggcttttctc ttccgcctcc gccgcctgcg
     9961 ggcgtttgct catccgccag ccggtaaagc caagcagggc gctgaacagc aaaaacggca
    10021 gatgcggcat ccccggcacc accgccagca cgaacatgat cccggtggcg gtgtagagca
    10081 ccgacgggct tgccagcaac tggtggcgca cgtcatgggc gatatcgccg ttgtcgctga
    10141 cgcgggtgac gataatcgcc gccgcggtgg agagcagcag ggaagggatc tgcgccacca
    10201 ggccgtcgcc gatggtcatc agcacgtatt gctggaaggc ggcgtcggcg ctcaggttgt
    10261 atttgaagat gccgatacag acgccgccgc tcaggttgat cgccagaatc atcatcccgg
    10321 cgatggcgtc tccgcgtaca aacttcgatg ccccgtccat cgcgccgtag aaatcggcct
    10381 cgctggcaac atctttacgc cgggtttgcg cctgcgcttg gttgatcaat ccggcgttaa
    10441 gatcggcgtc aatcgccatc tgtttgccgg gcatcgcgtc cagggtaaag cgggcagaaa
    10501 cctcggaaat acgctcggcc tctttggtga cgacgataaa gttgattgtc aacagcgtca
    10561 gtaaatccga gcgtgaaagc attatcgccg cgctgcacgg gcagtcgatt tttaacggtg
    10621 gtgggctgtc gccgttaaat aaaatcagcc cgtctcatcc gccaaaacct gccaccgtgg
    10681 cggtgccgga agagacggag aaaaaagcgc gtgatgtgaa tgagaaaacg gcgctgctga
    10741 agaagaaatc cgccaccgag cttggcgagt tggcaaccag tatcaacacc attgcccgtg
    10801 atgcgcatat ggaagcgaat ctggagatgg agattgttcc ccagggatta cgcgtgctga
    10861 ttaaagacga ccagaatcgc aatatgttcg aacgcggcag cgcgcagatt atgccgttct
    10921 ttaaaacttt gctggtggag ctcgcgccag tgttcgactc gctctataac aaaattatta
    10981 ttaccgggca taccgatgcg atggcctaca aaaacaatat ctacaacaac tggaaccttt
    11041 cgggcgatcg cgcgctttca gctcgtcggg tgctggaaga ggccggaatg ccggaagata
    11101 aagtgatgca ggtaagcgca atggcggacc agatgctgct ggacgcgaag aatccgcaaa
    11161 gcgcgggcaa tcggcgtatt gagattatgg tactgaccaa aagtgcgtcc gatacgctgt
    11221 atcaatactt tggtcagcat ggggataaag tggtgcagcc gctggtgcaa aagctggata
    11281 agcagcaggt gctttcgcag cgaatgcgct aaatgctgaa tctttacgca tttctcaaaa
    11341 cctgaaatca ctgtatactt taccagtgtt gagaggtgag caatgcgtaa aatcattcat
    11401 gtggatatgg actgcttttt cgcagcggtg gagatgcgcg acaatcccgc tctgcgcgat
    11461 atccctattg ctattggcgg cagccgcgaa cgtcgggggg tgatcagcac cgccaattat
    11521 cccgcgcgta aatttggcgt acgtagcgct atgcctactg ggatggcgct caaattatgc
    11581 ccacatctca ccttgcttcc ggggcgcttt gacgcctaca aagaagcctc aaatcatatc
    11641 cgtgaaatct tctcgcgcta cacctcgcgc attgaaccgt tgtcactgga tgaggcttat
    11701 ctcgatgtca ccgatagcgt ccattgccac ggttctgcga ccctcatcgc ccaggaaatc
    11761 cgccagacga tcttcaacga gctgcaactg acggcatctg cgggcgtggc accagtaaag
    11821 tttctcgcca aaatcgcctc cgacatgaat aaacccaacg gtcagtttgt gattacgccg
    11881 gcagaagttc cggcattttt acaaacctta ccactggaaa aaatccccgg cgtcggcaaa
    11941 gtctcggcgg caaaactgga agcgatgggg ctgcggacct gcggtgatgt acaaaagtgt
    12001 gatctggtga ttctgcttaa acgctttggc aaatttggcc gcattttgtg ggagcgtagt
    12061 caggggattg acgagcgcga cgttaacagc gaacggttac gaaaatccgt cggcgtggaa
    12121 cgcacgatgg cggaagatat tcatcactgg tctgaatgtg aagcgattat cgagcggctg
    12181 tatccggaac ttgaacgccg tctggcaaag gtaaaacctg atttactgat tgctcgccag
    12241 ggggtgaaat taaagttcga cgattttcag cagaccaccc aggagcacgt ctggccgcgg
    12301 ctgaataaag ctgacttaat cgccaccgcg cgtaaaacct gggatgaacg ccgcggcggg
    12361 cgcggtgtgc gtctggtggg gctgcatgtg acgttgcttg acccgcaaat ggaaagacaa
    12421 ctggtgctgg gattatgatg tatactatta tgtatattct ggtgtgcatt attatgaggg
    12481 tatcactgta tgcatcggat tctcgctgaa aaatcggtca atatcactga gttacgtaaa
    12541 aacccagcta aatactttat tgatcaacca gttgcggttc tttctaataa tcgccccgca
    12601 ggatatctct taagtgccag cgcattcgaa gcgttaatgg acatgcttgc tgaacaagag
    12661 gagaaaaagc ccataaaggc gcgctttcgt ccaagtgctg caagattaga ggaaattaca
    12721 cgccgcgcta aacaatatct taatgatatg acggatgatg atttcaatga ctttaaggaa
    12781 taaggatgcg ggtattcaaa acaaaactta ttcgcctgca acttacagca gaggaacttg
    12841 atgcgttaac ggcggatttt atttcctata agcgtgacgg tgttttgcca gatatatttg
    12901 gtcgcgatgc actctacgac gactcgttta cctggccatt aatcaaattt gagcgagttg
    12961 ctcatattca tctggcaaat gtgaataatc catttccgcc acagttgcgc caattcagca
    13021 gaacgaatga cgaagcgcat ttggtatatt gtcagggtgc gtttgatgag caagcatggt
    13081 tgctcattgc cattctgaag cctgaacctc ataaactggc tcgagataac aaccaaatgc
    13141 ataaaattgg aaaaatggca gaagcgtttc gcatgc
//
LOCUS       DAETPZ010000102        13157 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.102, whole genome shotgun sequence.
ACCESSION   DAETPZ010000102 DAETPZ010000000
VERSION     DAETPZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13157
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.102"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(67..2016)
                     /locus_tag="K0W59_003361"
     CDS             complement(67..2016)
                     /locus_tag="K0W59_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBI3024272.1"
                     /translation="MTDSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDATAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2101..2437)
                     /locus_tag="K0W59_003362"
                     /pseudo
     CDS             complement(2101..2437)
                     /locus_tag="K0W59_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
     gene            complement(2437..2793)
                     /locus_tag="K0W59_003363"
     CDS             complement(2437..2793)
                     /locus_tag="K0W59_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBI3024273.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2793..4289)
                     /locus_tag="K0W59_003364"
     CDS             complement(2793..4289)
                     /locus_tag="K0W59_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBI3024274.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4286..4447)
                     /locus_tag="K0W59_003365"
     CDS             complement(4286..4447)
                     /locus_tag="K0W59_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HBI3024275.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(4457..5019)
                     /locus_tag="K0W59_003366"
                     /pseudo
     CDS             complement(4457..5019)
                     /locus_tag="K0W59_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5016..5540)
                     /locus_tag="K0W59_003367"
     CDS             complement(5016..5540)
                     /locus_tag="K0W59_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024276.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRYARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERQRHWTQELLSS
                     ELQRSLRPVKRKHK"
     gene            complement(5506..5922)
                     /locus_tag="K0W59_003368"
     CDS             complement(5506..5922)
                     /locus_tag="K0W59_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBI3024277.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            complement(5919..6242)
                     /locus_tag="K0W59_003369"
     CDS             complement(5919..6242)
                     /locus_tag="K0W59_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBI3024278.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            complement(6287..8017)
                     /locus_tag="K0W59_003370"
     CDS             complement(6287..8017)
                     /locus_tag="K0W59_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBI3024279.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGHGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNGEVETLNGK
                     LTELENLKSDLEAELAEVKRPAGGTQNKVAGEHKEAFIGFMRKGREDGLRELERKALQ
                     VGNDEDGGYAIPEELDRTILTLLKDEVVMRQEATVITLGGSDYKKLVNLGGTTSGWVG
                     ETEARQETATSKLGLIEPFMGEIYGNPQATQKMLDDAFFNVEDWINSELALEFAEQEE
                     IAFTSGDGSKKPKGFLAYESTDEDDKTRAFGKLQHIASGAASGVTADAIIKLIYTLRK
                     AHRSGAKFMMNNSSLFAIRLLKDNDGNYLWRPGIELGQPSSLAGYGIVENEQMPDIAA
                     DAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQAIKLMKI
                     GAATRQKAAA"
     gene            complement(7995..9236)
                     /locus_tag="K0W59_003371"
     CDS             complement(7995..9236)
                     /locus_tag="K0W59_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBI3024280.1"
                     /translation="MFFQGLFQRKNNTPVTTPGMLAEELGLSYDTYTGKRISSQRAMR
                     LTAVYSCVRVLAESVGMLPCSLYKISGTLKTRAVDERLHKLISAKPNGYMTPQEFWEL
                     VIVCLCLRGNFYAYKVKALGEVVELLPIDPGCVEPKLNSQWQPVYQVTFPDGSVDVLT
                     QDEIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFGNGAVTSGVLRTEQKL
                     TPDAYERMKRDFEERHLGLGNAHRPMILEMGLDWKPVALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRESKQGKFY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            complement(9236..9418)
                     /locus_tag="K0W59_003372"
     CDS             complement(9236..9418)
                     /locus_tag="K0W59_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024281.1"
                     /translation="MFLIILTPLVGVLGALLLSYGTWLIYPPAGFVVAGALCLCWSWL
                     VARYLDRGHRVASGGE"
     gene            complement(9430..11187)
                     /locus_tag="K0W59_003373"
     CDS             complement(9430..11187)
                     /locus_tag="K0W59_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBI3024282.1"
                     /translation="MAKVADGIRYAERVVAGEIVAGEFVRLACQRFLDDLKYGEEWGI
                     YFSEPRAQHILNFYKFVPHVKGALAGQPIELMDWHVFILINIFGFVIPLVNEETGEVV
                     MRSDGSGRPVMVRRFRTAYNEVARKNAKSTLSSGIGLYMTGADGEGGAEVYSAATTRD
                     QARIVFEDAKNMVRKARSTLGRLFDFNKLAIYQEQSASKFEPLSSDANNLDGLNIHCA
                     IIDELHAHKTRDVWDVLETATGARLQSLLFGITTAGFNKEGICYEQRDYAIKVLRGYN
                     SDVEGAVKDDSYFAIIYTLDEGDDPFDETVWQKANPGLGICKRWDDLRRLAKKAKEQV
                     SARVNFFTKHMNVWVTAESAWMDMIKWEKCEYIAPRHELKTYPMWVGVDLAHKIDICA
                     AAKLWRTDNGHVHADFKFWLPEGRLERCSRQQAELYRKWAEMDKLILTDGDVIDHAQI
                     KSDLLEWIGGENLRELGFDPWSAMQFSLALAEEGIPLVEVPQTVRNLSEAMKETESLV
                     YAGRFHHSNHPVMNWMMSNVTVKPDKNDNIFPNKSTPEAKIDGPVAMFTAMSRMLVNG
                     GEPEPDLSEHLVSVGIRSL"
     gene            complement(11187..11669)
                     /locus_tag="K0W59_003374"
     CDS             complement(11187..11669)
                     /locus_tag="K0W59_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBI3024283.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDAKKTAKKDEKGLPKIPQHL
                     GAQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDVEGYTYRTETQ
                     NGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRAKVNIAGPDDVDPLAELLKARD
                     "
     gene            complement(11817..12167)
                     /locus_tag="K0W59_003375"
     CDS             complement(11817..12167)
                     /locus_tag="K0W59_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBI3024284.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(12210..12674)
                     /locus_tag="K0W59_003376"
     CDS             complement(12210..12674)
                     /locus_tag="K0W59_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBI3024285.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(12671..13157)
                     /gene="rrrD"
                     /locus_tag="K0W59_003377"
                     /pseudo
     CDS             complement(12671..13157)
                     /gene="rrrD"
                     /locus_tag="K0W59_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme RrrD"
BASE COUNT         2970 a         3884 c         3051 g         3252 t
ORIGIN      
        1 aaacaaactc cattgttagc atgttacgcg ataattttgc ctcatctttc accacaaaaa
       61 aagaactcac cttttaaatg gagaataccc aacatcatgg gtgattttca taaagggatc
      121 gcctgttttc ggcaaatcga taacgcgcat tccaggcggc gcgttgtcaa acgtcacctt
      181 aagttcgctg cgggttgccg gtgaagatgc aaggcttaac agctgattcc tttccagtga
      241 tacattcggg gtgtagcgtt tatcgggaga taaataccca tcaccacgtg gcatctgcca
      301 tccggtcatg tcatagacaa aatcatggaa ctgtttgccc cactcgtcga acttatcatt
      361 cagaccaaat ccactgttca gtgcatcagc cacaaaattt ttaacaagcc agggatgttc
      421 tttttcaaac tcctgcgcca tcatgcccag ttcaagcaat ccaccaatca gactgatctt
      481 tccgaggccc tttaatttca gccctgtttt tcctgccgcc gttgtccatt gattctgggc
      541 gactgttgcc gcacgaatac ttgccaccgc cttcacacca atatacaggg cagaaaccgt
      601 tgctattgtt tttactgcct gttcccatcc ccccattgct ctgacaacct gatcaacctc
      661 ctgccagact tttttcacaa ccggaccgac cgtttcccag ttatcaatta tgagatatgc
      721 accaccgacg agaagcgcaa ttaatccctt tgccggcgtc atattcatca cgctacccat
      781 gatttttgtt atgcgtgtca atgtgccaat cgccacgccc atcgtcagta atgccgcacc
      841 agttttcgcg atggttttta cgacctcagg attttctctg acaaacgtgc gcacttcttc
      901 cagaaaaggc ttcatctctt ttatgccttc attgagtgaa ggcaggaagg tttcccccag
      961 cgtggaagaa atcgcattga tctggttctg cagcagcaac aactgatttt ctgtcgtcgc
     1021 ggcacgggcg gcatattctt tctgcattga gccgccatac tgctgcgcat ccgccacacg
     1081 attaaagttg gttcgcaaca aatccagatt cgtgagcagc ggcgcaatgg cccccaggga
     1141 ttccttcccg aacagcgctt tcagcacagc agcctgtttt tctttcggca cttttgccag
     1201 agaatccagt acgtgcagca tggccccgcg ggcgtctttc tgcatatccg ccgccagttt
     1261 tttcgggcta atgcgcaacg agcggagcac ttttttctgc gatttggtgg cagaatccct
     1321 cgctgtcagc gacaacataa aattctttat ccccgtcgca gcaatttctg actccacccc
     1381 cattccggca atggttgccc ccattgcggc aatctctccg gaggccacac ccgcaacact
     1441 gcctaaaggg ccaatacggg tcacaacatc agaaatcttt ttcgcacttg caggaccggt
     1501 attgccaaga tagttaatct tatccgcaag ccctgccacc tctccctgtg tcagtttaaa
     1561 ggcagtgcgc cactgcgcca tcatctgacc ggattcttct gccgtggcgt caaaggccac
     1621 gcccatcttc acggcatcgt cagtaaactg catcagctcg tcacgggcga taccagcctg
     1681 accacccgcc gccacaattt cggcgatacc ttccgccgac atgggcagtt ctgttgacag
     1741 gtcgcgcacc tgctccgtca ttgccttaaa cgcttccggc gtatccagac catccaccac
     1801 tttccggaca tccgccattt ttgattcaag ggcaatggca gatttgaccg ggagcgccag
     1861 cgcccccagt actgcggttc cggcacccgc tgcgcccagt gacaggctgg caaactcctt
     1921 tttaaaacct ttcagctggc gctgcattcc cttcagcggt gccgatacct tatccacggc
     1981 agtgatgatc gccttcagct gaaaactgtc agtcatgctt catctcctcg ttaatgcgga
     2041 cggcctcggc ttccagttct gtaaactgcg aaacagccac ccgtcgcagc tccagtggat
     2101 tcagtttcca gaaccaggca acattgtaga gtcgtttccc ggaggtgctt cccgtctccg
     2161 actgggtaaa aaaacgcagg atctgcatgc tggtcttaaa aatatccagt tttgccatct
     2221 gcgccgccga tgaacgcggg atccccgcca gcaacgggat atatttcagt gccacccggc
     2281 tgtccagttt aatactgcct tcaccggaaa taatgaaagg aaaacccagc gcctcgattt
     2341 cgtcatacgt gggctcgcgc aactccagca catgcagcat ttcgttatgc gctgtcaccg
     2401 gtttttttaa aactatttct gtaacgttca ttgccattac tgataccctc cctcttcgcc
     2461 gtggaattca agatctgccg tgccttcttc ggcattatga ttagcctctc cgtgcagcca
     2521 tgccgccgaa agcacataca ccatgccgtt tgccagctca gcggtaatcg tcatctggtc
     2581 tgaggtggta attttgtcga ccggaaagtt tttgggcact ttaaacgtgc ccttaacgta
     2641 aggtgaccgc caggtctcct tgtaatccac atcccctgcc atgccgacga tgtcatcccg
     2701 gacattggtg ttcatcggca cttcaatgcc acctgtcagc gataactgct gaccatccac
     2761 tttaaaaaaa caggttccgg cgatctttgc cattatgctg actcctctga atactgaaga
     2821 cggaactggt taaccaccgc aaagacacgt aactgattaa cataatccgg cgggaacagc
     2881 gtattcagtc ggttcgggtt atctgcatca cgctcaacta tcagatactg tttaaacaga
     2941 tcgtaatttt ccacaatacc ggcacgctcc atctgacgat atgtcgccag aagttcccct
     3001 ttgataacgg caggagtgac aatcgcctgc cccggaccaa aacgggtacc atcatttgcc
     3061 agcttgtgac gtccgtactt gcttgtgatg accgatttca gtttgcgaag aacatatgca
     3121 ctggtatgca gagtttcact gtccagatag ctgttgtctg ccacgccata cgcattcttt
     3181 ttgtaggtgg ttacggaacg ctggatctgc aacgtgccgc cttccacata agccgtcgcc
     3241 acgccgtgag ataaaagggt ctgctgctct gtcatgatga atcgcttacc tttcggtgcc
     3301 ggaagcatcc ccaccagctc ccccgtctgt gtcggacggg caggatcatt ccggataaat
     3361 accgcttcac gggcaaggcg actggcaacc agttcatcga caggcgactg ggtttctttt
     3421 tcgtaaccgg caagcgtgat atgttgctga ttatgcatat ctccggcacc aaccagctct
     3481 gacagcgttc ccagttttgc ggtatagaca tgcccgtata actggcgcgc ataactccag
     3541 cgaccgctgc tgtcattcat ttcggtcatc atcatattga tggaggcggc atcgttgaac
     3601 ggcagaccga taaagtcgaa tgcctcatcg cccatagcag caacagcggc ggtaagatca
     3661 ggcgctccgc taccggcagc accgacttcc gtcaccaccc gaagccctgc tggcagaatc
     3721 tcaccaccac cagaaccata ataattcaga cagaccggta actcattacc atacagcccc
     3781 ttatgacggg cagtcagcgt caccacgcct gcatcagatg acgccgtaaa cggcagggtg
     3841 ataaccccgt ttaccgcttc cttaatcgcg gtggcaaccg cagtggcatc atcgccattc
     3901 accacaggca cctgtacacg ggagcgcccg acatacagac tcagggtgcc gctttcctct
     3961 gcttctccgg caaccgtcac cctgaccgtc gccgccgccc ctctggcttc cggtactgca
     4021 ataacataca gttcaccgaa aggatctgtc tgacggtaga cttccaccat acgcgccagc
     4081 tggctccccg ccccacaaat ctggcgggca taatctgccg atggcatcag caccaggctg
     4141 ttaacctcaa tggctgcatc gttgctggca tgcccgatca gtaatgcagg cgcgctggtc
     4201 accgctgtat ttgccgcaga attgttcatc tcggcgtaga acaacggcac cagcgtattc
     4261 gacggaacag tattaaaact tatcgtcatg attttttagc cttatcctgt ttaacacgta
     4321 ccacatcacc cgccgctata cggcggaacc agtagctgct ttcttccaca tttcgccctt
     4381 cggaaggcaa aaggtctcca cgggcagggt caggaaccga ccgccctttc aggggtttca
     4441 caaacatgag ggactcttaa ttctgaggga aacgcatttc cagatggtgc tcaatgtcac
     4501 catccgggcc agtaccagga tcgatgtaat ccacatcaat actcagcagg gataaatccg
     4561 gcaggcattc acgtcctctg cctgtcgcgt gtcttcttcc gtgatttcat acttcgccgt
     4621 aaaatcaaac tggtaataca gttcgtaacg gttcagatcc agcagggtgc caccgacata
     4681 aacaatctcg cccccttgcg ggtccggcat ccatccaagc agggctttcc agagttctct
     4741 ccggacatca tgaacggcgt cataggctgc ccactgccct ttttcatcac gctcattgct
     4801 gagcaccaca atcacggaga agccttcagt caaatcctgc cagtaatcgg tctgtgattt
     4861 ttgttccccc ggcgcatcgt cagagggaac cacataggca gcaggcaggc gaagttttcc
     4921 ggcatcaggg atcgccttaa actgcgccgc gccaccaaca cgctcctcaa aacgcgggca
     4981 tcgctcacgc agcgccgcaa ttatcgttgt cagtttcatt tatgcttcct ttttaccgga
     5041 cgtaacgaac gctgcagctc actggacaac agttcctgcg tccagtgacg ctgacgctca
     5101 ataacgtcag ccataaagtt attacgcggg gccagccgga aagtcgaaga atggtgcttc
     5161 ttctgccgct tatccttttt atccattcca tacgctgaat ggcgaacgcc gtaatacaga
     5221 aacgccggat aataaggagc accttcagga aaacggcgat tcccctgccc gtttttctgg
     5281 tcaggggaaa ttttcaccat cagtccgggg cgacgcgtcg tttttttggg aacgtaataa
     5341 ccaatggagc gggccagacg cccggtctga tatcccgggt tctctcccgg accggagcgt
     5401 ccgcgtttaa tcaccagacg tctggcataa cgcatgtaaa cgcgcccgat ttgcacaaac
     5461 gcccgacgca gacgggcgcg attaaactcc agctcctctg gttgtttaaa gtcgacgtgt
     5521 aaaaatgctg tctgattcat ggcattcacc ccgtcgtcgc gctgtacgca gttcttcaca
     5581 ttccagtaat aaaaaacgcc gctgaccgtt caggtcacgt attcgccgga tccggtactc
     5641 ttggccgtaa taaaccactt cgtgatctgc cgtgatgtcg tgccggaaac ggatcgtaaa
     5701 ataatgcgta acgatatttt ctgtctgcac tgagccctga taagcggcag cgcctggctg
     5761 agccaccttt gcccagacat caaacgactc cggatacgtt ggctccgtac caaaatcagc
     5821 ggtgggttca tccacacgaa ggcggatctt tatccggcga ttcagctctc cgggatccgg
     5881 taaaaggtaa gtggcactgg tctgactttg cctgattttc atagcgggac aatcctgtaa
     5941 gggccagcaa gccatctgaa actcattggc gtttccagtt tctccacatc ggtgatcgtt
     6001 gagcgatttt cataaaaatg actgaccagc atcagcattg ccagacgaac gtcatcagtc
     6061 agatgcatcc cgtcaggatc atcttccgga atcgtctctt ccggtgcata caacttcctg
     6121 ttcaggtatg tctctgtttt tttctgtacc gcctgtgcca gcagtttcag aaaatcgccg
     6181 tcactgtaca aaccatcatc gagccggaga tgagatttaa tctcctcttc tttcaggagc
     6241 atattttcct cctgtgcccg cgattacgcg ggcacaaaaa accgcattac gcagcggctt
     6301 tctggcgggt tgcagcccca attttcatca gcttaatcgc ctgagaatcc accagcatac
     6361 cgccggttcg cttggtggta taaaaaccca cgaacggttt gttggtgtac gggtcacgca
     6421 ggatacgggt accgatgcga tcaacgatgg tatagccgcg tttgaagttc ccaaacgcaa
     6481 tggcttttgc atcggcggca atatccggca tctgctcatt ctcaacgatg ccataccctg
     6541 ccagagaaga aggctgaccc agctcaatac ccggacgcca caaataattt ccgtcgttat
     6601 ccttcagcag acgaatggca aacaggctgc tgttgttcat catgaacttc gcgccgctgc
     6661 ggtgcgcctt acgcaaggtg taaatcagtt taatgatcgc atcagcggtc acgccggaag
     6721 ccgcgccgga agcaatgtgc tgaagtttgc caaacgcacg ggtcttgtca tcttcatcgg
     6781 tggactcata agccagaaaa ccttttggtt ttttgctacc gtcgccactg gtaaaggcaa
     6841 tttcttcctg ttcggcaaat tccagcgcca gctcactgtt gatccagtct tccacattaa
     6901 agaaagcatc atcgagcatt ttctgtgtgg cctgcgggtt gccgtagatt tcccccataa
     6961 agggctcaat cagtcccagt tttgaggtgg cggtttcctg acgcgcttcc gtttccccca
     7021 cccatccgga cgtcgtgccg cccagattca ccagtttttt ataatccgag ccacccagag
     7081 tgattacggt ggcctcctgg cgcatcacca cttcatcttt cagcaacgtc agaatggtgc
     7141 gatccagttc ttccggaatg gcataaccac catcttcatc attacccacc tgcagtgcct
     7201 ttcgctcaag ctcacgcagg ccatcttcac gccccttgcg cataaaaccg ataaacgctt
     7261 ctttatgttc cccggcaact ttattttgcg taccacctgc cggacgctta acttcagcca
     7321 gctcagcctc aagatcgctt ttgagatttt ccagttcagt cagttttccg ttgagggttt
     7381 ccacttctcc gttcagtgca gagcccacat cctcagcgtc gcgcagggac agtgcgccat
     7441 agcccccggc catgaatgct ttggcctggg tacgggagag tccgacatca cgcaggactc
     7501 tttcgatttt tttctgttcg gggatttccc cgcgggccag tgcgttcttg acgtcgctga
     7561 tccgcgcctc gtcgttagac gggaacgtca ccagactgac ttcccagagg tcgatttctt
     7621 tcagcagaaa ggcttctttg ctccggtcgt attcccagtc tttcaggacg tacccaatag
     7681 aaaggccggt taacgaaccg gccttcatgt gtgcatgtgc acgttttgcg aggggatcat
     7741 catcaataag caaccgtccc ctgacgtaaa gcccgacatc gtcttccttc atttcggtgt
     7801 aaacaccgat gggttcatcc atgcggtgct gccagagcag cgcaggtaac gcttttctgt
     7861 cactccacgc ccgcagggaa gcagcaaatg ccccggacat caccacatca tcgtggctgt
     7921 cctttacacc aaagacggag ccatgccctt caaactcacc ggagtcactg acagatttca
     7981 gactcagcgg tacatcaaga cgttgtttcg tctgcattgg cgttatcctt ctgcttaccg
     8041 gctttactgc catcggaggg tttcgtggtc atgttcatcg gtgtgagata gacatcccca
     8101 ccgggacgcg gattcatatc ttccaggtcg cggcagtcat tgggagagta aatcccccag
     8161 ttaatcccgg tggcgtaggc ttcaaaacgg gatttcatat ccccgcgcag taacgccccg
     8221 gcgttaaatt tggcgtaaaa cttcccctgt ttgctctccc tgaccagccc tgtattgatc
     8281 cgttgttcaa tacgggtcag atacggcaca agggaatagt tgataaatcc gagccccagc
     8341 tcttcaatat tgttgaaagt ggcgcgatcg gtgttctgca ccatgtgcag cggcacgcgg
     8401 aaaagacgac agatttcttc caactgaaac ttgcgggttt ccaggaactg gctgtcctcg
     8461 gcattcagtg ccaccggctt ccagtccagc cccatttcca gaatcatcgg acggtgcgca
     8521 ttacccagcc caagatgacg ctcctcaaaa tccctcttca tgcgctcata agcatccggc
     8581 gtgagctttt gttccgtgcg caacacaccg gatgtcaccg caccattacc aaacaacctg
     8641 gcaccgtgct cctcggttgc cgctgccagt gaaatggcct cgcgcgcata tgcaatggga
     8701 ttcagcccga ccagtccgtc cagcgtcagg gtgcgcacat gccagatttc atcctgggtc
     8761 aacacatcca cggaaccatc cggaaacgtc acctgataaa ccggctgcca ctggctgttc
     8821 agcttcggtt ccacacagcc cggatctatc ggaagaagct ccacaacttc ccccagcgcc
     8881 ttcaccttgt aagcgtaaaa attcccccgc agacacaggc agacgataac cagctcccag
     8941 aattcctgcg gcgtcatgta gccattgggt tttgctgaaa tcagcttatg cagccgttca
     9001 tccaccgccc gtgttttaag ggtgccgctg attttgtaga gactgcaggg cagcatacca
     9061 acagactcag ccagcaccct gacgcaggaa tagaccgccg tcagccgcat ggcccgctgg
     9121 ctgctgatcc gctttccggt ataggtgtcg tatgacaacc ccagctcttc cgcaagcatc
     9181 ccgggcgttg tgacgggggt gttatttttg cgttgaaaaa gcccctggaa aaacattact
     9241 cacctccgga ggcgacccgg tgaccgcgat cgagataacg cgcaaccagc cacgaccagc
     9301 acagacacag cgccccggca acaacaaaac ctgccggggg ataaatcagc catgtgccat
     9361 acgacagcaa aagcgccccc agcacgccca ccagtggcgt gagaattatc agaaacataa
     9421 tgacctcggt taaagcgagc gaataccaac gctgaccaga tgctcagaca gatccggctc
     9481 cggttcaccg ccattgacca gcatccggct cattgctgta aacatcgcaa cagggccgtc
     9541 gattttggct tccggcgtgg atttgttcgg gaagatattg tcgtttttgt ccggtttcac
     9601 cgtaacgtta gacatcatcc agttcatgac cgggtgatta ctgtgatgga aacgcccggc
     9661 atagaccagt gattccgttt ccttcatggc ctctgacaga ttgcggaccg tctgcggaac
     9721 ttccaccagc ggtatacctt cttcagccag cgccagactg aactgcattg cgctccacgg
     9781 gtcaaatccc agttccctca ggttttcacc gccaatccat tccagtaagt cactttttat
     9841 ctgagcatga tcgataacat caccatccgt cagaatcagc ttatccatct ccgcccactt
     9901 ccggtaaagt tctgcctgct gccgcgagca tcgttccagc cgtccttccg gaagccagaa
     9961 tttaaaatcg gcatgaacat gcccgttatc cgttcgccag agttttgccg ccgcacagat
    10021 atcaatctta tgagcaaggt caacgccgac ccacatggga tacgttttca gctcatgtcg
    10081 tggggcaatg tattcgcact tctcccactt aatcatgtcc atccaggcag actctgctgt
    10141 tacccacaca ttcatgtgtt tggtaaaaaa attcacccgc gcagagacct gttctttcgc
    10201 ttttttcgcc aggcgacgca gatcatccca gcgtttacag atgcccaggc cgggattcgc
    10261 tttctgccag accgtttcat caaacggatc atctccctca tcgagggtgt aaataatcgc
    10321 aaagtaggag tcgtctttta ccgcgccctc cacatcgctg ttatagccac gcaatacctt
    10381 gatggcgtaa tcacgctgct cgtaacaaat cccttccttg ttaaaccctg ccgtggtgat
    10441 accaaataaa agggactgca gacgggcacc ggttgccgtt tccagaacgt cccacacgtc
    10501 acgggtttta tgtgcatgca gctcatcaat aatggcgcag tggatgttca gaccatccag
    10561 gttgtttgca tctgaagaaa gcggttcaaa ttttgatgcg ctctgctcct gataaatcgc
    10621 cagcttgttg aaatcaaaca accgcccgag tgtcgaccgg gcttttctga ccatattttt
    10681 ggcgtcttca aacacgattc tggcctggtc acgcgtggtt gcggctgaat acacctcagc
    10741 cccgccttca ccatctgccc ccgtcatata cagaccgata cctgatgaca gggttgattt
    10801 tgcgttttta cgggcgactt cgttgtacgc tgtccggaaa cggcgcacca tcaccgggcg
    10861 tccgctgcca tcgctgcgca tgacaacttc cccggtctct tcattcacca gcggaatgac
    10921 aaaaccaaaa atattaatga ggataaacac atgccagtcc atcaactcaa tgggctgacc
    10981 tgccagcgcc ccttttacat ggggcacaaa tttgtagaaa ttcaggatgt gctgcgcacg
    11041 gggttcactg aaataaatcc cccactcttc gccgtacttc agatcatcaa gaaaacgctg
    11101 gcaggccaga cggacaaatt caccagcgac aatttctcct gcaacaacac gttcggcgta
    11161 gcggatcccg tcagccactt ttgccatcag tctctcgctt ttaaaagttc tgccagcgga
    11221 tcaacatcat ccggtccggc gatatttact ttcgcccggc ttgccggtga cataccaaat
    11281 tctgcaagca tcgcccggat ccgcttccag gcatccgctt tcatcgcagc agccgggtgt
    11341 gccttaatca gcacatcacc gttctgcgtt tccgtgcggt aggtataacc ctcaacatcg
    11401 agggtttcgc agtgatgccg gtattcggtg taggcttcca ccagcaactc gagtgcacgc
    11461 gcatcaagct gagaaatgat cccttccgca ttcagctctt ccgccattcg cctgaaccag
    11521 tactttccct gcgcccctaa atgctgcgga attttaggga gacctttttc gtccttttta
    11581 gcggtttttt tggcgtcttt aacgggacgc tttgagggat tgcctcgtat caaatgcagg
    11641 cgtggcgggg ttttcggggg tcctgacata atcgatttta cctatcaatc gcttgatcgc
    11701 attcccaaaa aaaagttttc gaacctgcgg cgatgcgagg aagggttggc gggcggtccc
    11761 ggacagccag ggctgcaggg atttgacccg cccctcccta caagtgagaa taattatcac
    11821 ctgattcgtt cgcgcgctgt tttcgcttta tggcagggcc agcacagact ctgcaggttg
    11881 ctgtctgcgt ctgttccgcc atgcgctttc gggatgatgt ggtcgacggt tttcgcctcg
    11941 ctcaccacac cgacacgcag acacaactga cacagacctt tatcgcgctt cagaatacgg
    12001 gcacgaatca ccgtccattt tgagccatag ccacgctggt ggcggctcag tccgcgctgg
    12061 tgctgcaccc agccttcacc gcgatgctta tcgcagtaac ccgaactgtc tgtggttgta
    12121 cctgcacatc cacgcttacg gcaggcacgt gggattagtg atggcataaa tacctcatac
    12181 cctgcgaaat gtttaccacg ataaaaaggc tacttaatgc actgagtgcg gatatactcc
    12241 tgtgcccctt ccagttgcat ctgcatcgtc atcagccgct ctctgagggt gaaataatcc
    12301 cgttcagcgg tgtctgccag tcgggggctg gttgcattat ccacgccgga ggcggtggtg
    12361 gcttcacgca ctgactgaca gactgctttg atgtgcaacc gacgacgacc agcggcaaca
    12421 tcagcgcgca gagtttcatt ttcagctttc gcatcagcta actccttcgt gtatttagca
    12481 tcgagtgcat taacatcacg ctgtctctgc tgcatgtcag taatggttgc gttcgccagc
    12541 ttcagttctc ttgcgttttt gtcgcgctgc tctttgtagg taatggcgtt atcacggtaa
    12601 tgattaacag cccatgacag gcagacgatg atgcagataa ccagagcgga gataatcgcg
    12661 gtgactctgc tcattgctgc ccccacaaac agacttcacg ctcaatctca cgacgggtca
    12721 tcagcccttt ccattgctta ccgccagcat atgtccagcg acgtagctgg tcacatgcgc
    12781 ctttgatatc gccctagttt attttgcgaa gaagcatcga tgttctgaaa ttgccagcgc
    12841 ccacgttgta aacgaacgag taaagagcgc cgcgcgttgt ttccggtata tcgactttga
    12901 tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt attcaggagg gctttgcatt
    12961 ctgcttcggt atacgtttta ccgagcatga tgtcttttcc ggtgtgtccg tggcatacag
    13021 tccatacgcc aacgatatct ttgtatggta tgtagctgac accttccaga ccatcgttac
    13081 cacctgggcc agtgatgagc acagacgcta tggcaatagc cccaccacca atagcaactg
    13141 caacagcctt gcgtaat
//
LOCUS       DAETPZ010000103        13010 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.103, whole genome shotgun sequence.
ACCESSION   DAETPZ010000103 DAETPZ010000000
VERSION     DAETPZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.103"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..606)
                     /gene="capU"
                     /locus_tag="K0W59_003378"
     CDS             complement(<1..606)
                     /gene="capU"
                     /locus_tag="K0W59_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HBI3024286.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGHEF"
     gene            complement(609..1451)
                     /locus_tag="K0W59_003379"
     CDS             complement(609..1451)
                     /locus_tag="K0W59_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBI3024287.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            1637..2833
                     /locus_tag="K0W59_003380"
                     /pseudo
     CDS             1637..2833
                     /locus_tag="K0W59_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2916..3173
                     /locus_tag="K0W59_003381"
     CDS             2916..3173
                     /locus_tag="K0W59_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024288.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(3731..4498)
                     /gene="fecE"
                     /locus_tag="K0W59_003382"
     CDS             complement(3731..4498)
                     /gene="fecE"
                     /locus_tag="K0W59_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HBI3024289.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(4499..5455)
                     /gene="fecD"
                     /locus_tag="K0W59_003383"
     CDS             complement(4499..5455)
                     /gene="fecD"
                     /locus_tag="K0W59_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HBI3024290.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(5452..6450)
                     /gene="fecC"
                     /locus_tag="K0W59_003384"
     CDS             complement(5452..6450)
                     /gene="fecC"
                     /locus_tag="K0W59_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HBI3024291.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(6447..7349)
                     /gene="fecB"
                     /locus_tag="K0W59_003385"
     CDS             complement(6447..7349)
                     /gene="fecB"
                     /locus_tag="K0W59_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HBI3024292.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(7394..9718)
                     /gene="fecA"
                     /locus_tag="K0W59_003386"
     CDS             complement(7394..9718)
                     /gene="fecA"
                     /locus_tag="K0W59_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HBI3024293.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPIS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(9805..10758)
                     /gene="fecR"
                     /locus_tag="K0W59_003387"
     CDS             complement(9805..10758)
                     /gene="fecR"
                     /locus_tag="K0W59_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HBI3024294.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(10755..11276)
                     /gene="fecI"
                     /locus_tag="K0W59_003388"
     CDS             complement(10755..11276)
                     /gene="fecI"
                     /locus_tag="K0W59_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HBI3024295.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            11574..11756
                     /locus_tag="K0W59_003389"
                     /pseudo
     CDS             11574..11756
                     /locus_tag="K0W59_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11750..11854
                     /locus_tag="K0W59_003390"
                     /pseudo
     CDS             11750..11854
                     /locus_tag="K0W59_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            12194..12451
                     /locus_tag="K0W59_003391"
     CDS             12194..12451
                     /locus_tag="K0W59_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HBI3024296.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
     gene            12781..>13010
                     /locus_tag="K0W59_003392"
     CDS             12781..>13010
                     /locus_tag="K0W59_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3024297.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQSEYDSQWATICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLKEL"
BASE COUNT         3195 a         3361 c         3409 g         3045 t
ORIGIN      
        1 aaattcgtgc cctttttccg ggcgcaacat tcctacctga gcaattacgg ggcatcctct
       61 tcgggacgcc agccaggaaa gaacactcgg aggcaaactg tttcgggaat caacgtataa
      121 ttcctggaaa tcaaagccag gaggcacaac agtaacccga gttcgacatc cttcctgctc
      181 cagatgtgtc ttcatatttg ttccgggaac aatcacctca tcgcaaaaat gatttattga
      241 aaaaaatttt gtttttcgtg tcaaatatgt cttttgcctg ataattctga atggatgttt
      301 ccaggtaaaa aaccgtacca aaccaacaat attactatca tgtccactgt gacaaacgat
      361 tgcatcgggc tgaaaactat gaactattcc gagtaatctc catacagtag gaatgtgaag
      421 actgtttcga aataacgcga atgtgatatc aattccaaac ttactggctt caaaagcaat
      481 tttgctgttc tccctgcaga caagcagaac tgaatgcccc attttcttca gggccttcat
      541 ttgagcaaca gcctgtaatt cctgaccacc tatattcggt gatgattccg taaatagtat
      601 attcattatt agtcatccgg taatcttggc cccttatgaa gggccagcaa cgatgaaaaa
      661 aacggtgttg tataataaaa aagtcgtctt ttcagccata cgcaattttc ccgttccttg
      721 gtgcttattc ttcctatccg tgtagttcct ttagcaggag cattcattct tctttctgtt
      781 gtatataaat aatggaatcc aagttcttca gcgatctgaa tataatcctt gttataataa
      841 ccttccggcc aacacaaatg tttactgcat tttcctgtca ttttttttaa atattctctc
      901 ccgctcaata agtcctgccg aagatgttta cactgttctt ctcgtgaagt gaacttttta
      961 tcccaacgtg tatgagtatg tgtatgaaca tgaaattcga ccagaccaga ttgcaacatt
     1021 tcgttcactt cagaccagcg aagcataaca ttatcagcat tcccggttgc tatttgatgt
     1081 tcgcaatcgc gatgagaata ttcctttcca ggagagtgac gaaccggacc atttccaata
     1141 aaactggtaa ttaaaaaaat atgtgccttg agattaaact cgtttaataa aggatatacc
     1201 tgaaaccagt tgtcaaggta tccatcatca aatgtcaaca taacgctttt acggggaagt
     1261 tttccgcccc gataaaaaaa ctccagttca tctgatgaca gtgttttcca gttgttttca
     1321 gccaaccatt tcatttgttt ccggaatgtc tcgggagaaa gtgtaaccag tcctgggcaa
     1381 tggctgacat gatgatacat taataccgga acatgatttg ctttaaacaa aatgccccct
     1441 ccgtttaaaa tttaaagttt ctggcaccca gtaagtagag ataaaaggaa agcctaataa
     1501 aggtggtgaa ctttatatac tactaaatca aacatcaaca atttataaac aataatatat
     1561 tatcatatct tttaaaaacg gctcactaag aaccttgagg tggcacacta aatttagcta
     1621 cctgaacaga ggtgatatgc tcacctcaga acaacacagg tgccataatg aaaaaagaaa
     1681 tttcagcgca gagtttaaac gcgaatccgc tcaactggtc gttgaccaga aatacaccgt
     1741 ggcagatgca gccagcgcta tggatgtcgg cctttccaca atgacgcgat gggtgaaaca
     1801 attacgtgat gagcggcagg gaaaaacacc aaaagcctcc cccattaccc cggaacaaat
     1861 tgaaatccgt gagctcagga aaaagctaca acgtattgaa atggaaaatg aaatattaaa
     1921 aaggctaccg cgctcttgat gtcagactcc ctgaacagtt ttcgataatc gggaaactca
     1981 gggcgcgtta tcctgtggcc actctctgcc atgtgttcgg ggtccatcgc agcagctaca
     2041 aatactggaa aaaccgtcct gaaaaaccag acggcagacg ggctgtatta cgcagccagg
     2101 tacttgaact gcatggcatc agccacggct ctgccggagc aagaagcatc gccacaatgg
     2161 caacccagag aggctaccag atggggcgct ggcttgctgg cagactcatg aaagagctgg
     2221 ggctggtcag ttgccagcag ccgactcacc ggtataagcg tggcggtcat gagcacgttg
     2281 ctatcccgaa tcatcttgag cgacagttcg ccgtaacgga accaaatcag gtgtggtgcg
     2341 gtgatgtgac ctatatctgg acgggtaagc gctgggcgta cctcgccgtt gttctcgacc
     2401 tgttcgcaag aaaaccagtg ggctgggcca tctcgttctc gccggacagc aggcttacca
     2461 tgaaagcact ggaaatggca tgggaaaccc gtggtaagcc cgtcggggtg atgttccaag
     2521 cgatcaaggc agtcattata cgagcaggca gttccggcag ttactgtggc gataccagat
     2581 cagacagagt atgagccggc gtggaaactg ctgggataac agcccaatgg agcgcttctt
     2641 caggagtctg aagaactaat gggtgccagc gacgggctat gtaagcttca gcgatgcagc
     2701 tcacgcaata acggactata tcgttggata ttacagcgca ctaagaccgc acgaatataa
     2761 tggtgggtta ccaccaaacg aatcagaaaa ccgatactgg aaaaactcta acgcggaggc
     2821 cagttttagt tgaccacaac agactacctg aagggagccg cggtcgccta gcagttgcag
     2881 tagcaggaga tcatccagcc gcagtacagg tcacgatgac tctggttaat gataccggct
     2941 ttgaccccgt attttccggc tctatcgctg aatcatggcg tcagcagccg tgcacaccat
     3001 cctattgttg tgactgggag gctgccacca tgcttcgcgc tttccctctg gcgaaaaagg
     3061 gagaaggacg ggcccgtctg ccttcacttt atgccagctt cggtaagctg ggtgagacac
     3121 cgactcatga agatatcatt gataacaatc gatccatcaa ctggcctgta taacgtggct
     3181 gccggtgatt aagaaagctg cacctaccta agtagtagca aacgcacact ttttagaaaa
     3241 atcgatggtc agaaactgga ttagcaattc cgttccaggg ttgcttttga tttacgttgg
     3301 cgtctgatca ttgatttatc ctcaaaagcc caacctcatt ggtaatgaac cagctccgtg
     3361 aatgtccgct ctggcacaga gcgaaatttt ttgatctccc cccctgaaat ctaaacttag
     3421 tcatgtcacg tttttgggtt tctaaaattt taacttcgcg tttttcgttg ccgtaagggt
     3481 tatacagaaa tgtccgttaa gcagagttca aaattgattg ccgtgatcac gactggtttg
     3541 aaagccgcgc ccaagcctgt acagctctgg tttgcgttga ttatgaacct gtcagcctaa
     3601 agcaagcgga tggacgatga gtattggtaa tctttcagag tccggaaaag ttcagcccca
     3661 gtctgaacag gcttgctggc gccagtccag tttcattcag tcgtggtttg gttcttacgg
     3721 cctgtgcaat ctacctcatt aggcacatcg gcctgccaga taccggctcg gggtgtattt
     3781 ccgcttccac gctgaatact gttctcagca atcctggggt catcacctct tctggtgtgc
     3841 cttgcgccat aacatgtccg tttgccatta ccaccagttg atcgcagtac cggctagcct
     3901 gattaaggtc gtgcagcaca gcgaccaccg ttttcccctg agtccggagt tcgcccatca
     3961 accgcatcag gtccacctgg tgattgatat caagataggt ggttggctca tcaagtaata
     4021 caacgggcgt attctgggcc aggaccatcg ccagaaatgc gcgctggcgc tgaccgccgg
     4081 aaagctcggt taaccgacga acggcaagat gattgatccg ggtctggttc atggcgacat
     4141 taactcgtgc attgtcttca gcggagagac gcccccagag tgacagccag ggattacgac
     4201 catacgaaac cagctcctgg actgtgatcc cctctggcgt taaatggtgc tgaggcagca
     4261 gcgaaagcct gcgggccaac tggcgtgatg agagcatatt tatgggatta tcgccgagaa
     4321 atacggtgcc agactgcggc attaaaagcc gcgaaaaaca gtttaacagc gtcgatttcc
     4381 cgcaaccgtt agggccgatc agggcggtga tcttccccgt tggcagtgag agtgaaacgt
     4441 cgttaagtac cttgtctgtc ccgtaactga ccgtcagatt ttcagttcgt aaagtcattt
     4501 atcgcattct cacaagcaac cagacaaacc acggcgcacc gataatggcg gtcagcacgc
     4561 caaccgggag ctccagtggg ggatgaataa ttctcgccag cagatcggca accaccaaca
     4621 gcaacgcacc tgtcagggct gaaacaggca gcagtctgcg gtgacgtcca ccggtgatgc
     4681 tacgcatcat atgcggcacc acgagaccaa taaagctaat cgggccgcag gcggccacgc
     4741 cggtagatgt catggcgaca gctagtaaca aagcccagaa tcgggtatgg ggcaccgaca
     4801 caccgagcgt ggtggcgcgc gcatcgccga gtgcaaggag gtcgagatcg cggcaaaaac
     4861 tcaggctcag cggcagaaat aaaatcatca gcgggatggc aatcttcaca aagctccagt
     4921 cacggcccca taagctgccg gtcagccaca gcagggcgtt gttcacatcc tgcgggcgcg
     4981 agagcatcag ataatccgtc aggctggccc agcatgcaga aagcgccacg ccggtgagcg
     5041 ccagcttcat cggctggtgg gtctttgcca gcatcttcag taatatcaac cccgccatgc
     5101 cgcccgcaaa ggccagcagc ggcagcacca tcacgggcag tgacggcata agaagtagag
     5161 cccccacaga ggccaggctg gcggcatggt taacgccgag aatatccggt gatgccagag
     5221 ggttgcgcac aatcccctgt atcagcacgc ccgccacggc gagggctgca ccgacaaaca
     5281 gtgccagcag caagcgcggc agtcggtact ccatcaatac ataataatgc tcgcgtccgg
     5341 cctgccagtc ggtcagcagc gcgcgccacg gcacggggat cactcccata tggagtgata
     5401 acagcgcaca gcccgccagg gcaagggtga tgaaaataac cagcgcaatt ttcatcctcg
     5461 cctcctcaca agccagacaa agcaagggct gccaatcagc gccagcactg cgcctgcggg
     5521 cagatctccg gggaaggcca gcgcgcgtgc gagtacatct gccagcagca tcagcgtggc
     5581 ccccagcagc atgctcactg gcagtacgtt gcgctgatcg aagcctgccc agaagcgcgc
     5641 cagatgtggc accagcagac cgataaacgc caccggacct gccacgctga cgcacgcacc
     5701 aaccagaagc agcactaaca tattgatgac caaacgtagc ctcgtcaggt tcactcccag
     5761 cgtatgggcg gtgctgtcgc tgaggttgag caggttcagt tgattcgcca gcagtaacac
     5821 gacagggact gcagtgacca ccaccggcaa gagctgccag acatcctgcc agcgggcgtg
     5881 ggacactccg cctgccagcc agtaaaagat gccgtaagca tgatcttcgg ccagcagcag
     5941 ggtgatgcgg gtaaggccca tacaaaaggc cgacagcgcg atacccgcga ggatcagttt
     6001 gtttctgtca tgggtatgac gaaatccgcc tcctgcggtc atgaccagca gccagctcac
     6061 gccgcccccg catgccgcga tgaacgacag agaatagcct gcaatcggcg tcggactcag
     6121 cgcgctggta agcgccatag ccagcgccgc gccgctgtta atgccgagca gtgaaggaga
     6181 ggccattggg ttgtgggtca gggtttgcag cagcgtgccc gcgagcgcca ggcttgcgcc
     6241 gatcagaacg gcgaccaggc ttcgtggcaa acgaaggttt tgcaccagcg cttctggtag
     6301 cgttggcgtg tgtccaggca gcagggcgcg ggttgcatct gctccggaaa caggaatggc
     6361 cgagtagcaa aacagactca gccagaaaat aataataagt gctgcaacgg gaagccccca
     6421 cagcagcatc gggtgtttta tcgcggtcat ttcacaacgg taagcggctg atggtggaag
     6481 atttttaccg tgtcagcggc aatacgctct gcagcaaaaa taccgcgcat ccgcgcccag
     6541 gtgttactgt cgaccgaagc aacctgctgc ttctgcgcgg cggttaacat ctgccagagc
     6601 ggatcttgtt gccagcgttt aacaatgctc tcttcgcgat agtgggcaac cagcagccag
     6661 gcaggattga ccgccagcag ttgctccagg ccgatggacg gcatggacga acccgccatc
     6721 gcagcgggaa cgttcagccc cagagaggcc agcacgctgc cggtccaggt ctcctgagta
     6781 tgcaggttga attgctgttc gcgtgatgtg ccaaaggcca cgcgtgtccc tttgggaagc
     6841 tggctggccc actgcgccat cctctcttta tgttgttcca gacgtgcctg catctctcgc
     6901 tttttaccca ccatttcgcc gatgatagcc gcagattgca aattttcagc gtaggtttcg
     6961 ttgcgggact taagcagcag taccggcgcg atttgctgca aggcgatgta aacccccgca
     7021 tggcgactgc tgtcggcaat gatcaggtct ggtttcagag cggcaatggc ttccaggctc
     7081 ggctgcgcgc gcgttccgac ggactgccac ggtttcaggt gcgcacgcac ttcgggcagg
     7141 atgcgttttg catcgttatc gtcggcaata ccgatcgggc tgacgtccac ggcggccagc
     7201 gcatcggcga acgagagttc cagcaccaca atccgttgtg gcgttttttc gagtgtaaac
     7261 gtgccgtgtt cgtcctgaac catggcggca aaggcgtggc tgatcaccag cagcaggcct
     7321 gcaaaaagaa aacggataaa tgccaacata atcacattcc agctaaaagc ccggcaagcc
     7381 gggcgttaac acatcagaac ttcaacgacc cctgcatata cagcgtgcgc ggctggcctg
     7441 catagatgcc tttgttgttg tcgtcataag agcggatgaa gtagtcctgg tcgaagatgt
     7501 ttttcacacc gaatgccagg ttcagatctg ccatctgcgg gccaaagtca tacgccacgc
     7561 gtgcacccca gagcatgaag ccgggaatgc ggccggtact gccgtcggcg ctctctttca
     7621 ccgtattggc gttatccgca aactggctgg actggaaatc gctgttcaga ttgaacgtcc
     7681 agtttcctgg cttgtagtcc acgcccagcg tgcctttatg tttcggggag aatggtacca
     7741 gattgccgta ggtgtcgcct ttctcgcgga tttccgcgtt cacatacgca tagctggcgt
     7801 agatggaaac gttatcaagc gttggcgtta gcgtacccag atcgtaacgt gcctgcgttt
     7861 ccagcccggt atggcgcgtt ttgccacgtg cagtgacggt gtcgttggtc tggttggagt
     7921 cgtactgatt gttaaagtta atcaggaaca gccccatttc cgccgtcagc gcgccgtcgt
     7981 cgtagcgggt accgagttcc caggttcgcg ctttttccgg ttccacattg ccgctttgca
     8041 cagccttgcc aatctggctg tactgtacgg tgccgaacga gccttcagtg tttgcataaa
     8101 gattccagct gtcagtcagg tgatagagca cgttcaacgc cggaagcggt gcgttatagc
     8161 tcacttcttc gtgcgtgcct gtgatggcgt tgttctggta tgactcgata tgttcgaaac
     8221 gcatacccgg cgtgatggtc cagttgccga tgtcgatttt gtcatccaga taccaggcgt
     8281 gcgcctcggt gccggaacgc gtatcgcggt cgtaagggct tgagccggac ggcaactgcc
     8341 cgctgctggt ggcggtgtag taacgcattt catgcgttga ttcattcaca tagcgatagc
     8401 ccacgcccac ttcgtgcgcg gaagggccga tcataaagat ctggctgtag cgtggctcaa
     8461 taccgcgcac ccagtagtta cgcggcgaga gggtgatgcg tttgccttgc tccaggtagc
     8521 cgctgcgcag ggtttgggtg tagaacccct gaatgttgaa tttatgctgg ctgtctggct
     8581 ggaactggta gcccaggctc gccagcttgc gacgacccca gaagcggtca tacgggcggg
     8641 tggattgcca gcgatcggcg tcgtaatccg cgcgagacag gccaccgggc atgtcggctt
     8701 caccgtcgta atattgcagc aggctgttga aggtgtgcac ctcatccggc gcatatttgc
     8761 ttttcagcat caggtcgtcg atgcgggtgg cgctgtgctc gcgccagtca ctgccgcgcg
     8821 tgccggagta gagcagcgcg gtgccaaaac cgttgtccgc tgtgccgccc accatcaggt
     8881 tgtgcgtctc tttcgggttg ttttgtgaag aggttgggct gagctgacct tccacgcccg
     8941 cctcgatacc aaagtcctgc ggaatggcac gggtaacaaa gttcaccacg ccgcccacgc
     9001 tctgcggtcc gtaacgcacc gcaccaccac cgcgtaccac gtcaatggca tccatgttgc
     9061 cgagcgaaat gggagccagt gaaagctgcg gctgaccgta aggggcgaag gggacgggga
     9121 tgccgtccat caggacggtc gagcggctgg cgaggcgcgg gttcaggccc cggatgccaa
     9181 agttcatcgc caggtcgtgg ctgccggtgc cgttgttttc cggcgcgctg acgccaggga
     9241 tgcggttaag tacctcacgc atggtggttg cgccggtttt ggcgaaatcc tcacggcgga
     9301 tcacgtcacg cgcgccagca tgttcaaata cgtcgttttc acgcgcatca cccagccagt
     9361 cgccgaccac ggtcagggca tcttcttttg gtgcgggcgc gggctccagc gtccagctgt
     9421 tatttcccag cggttttacc tgcagtccgc tgccgtccag cagttgttgc aggccgctct
     9481 cgacgtcgta atcgccgtgc aggccgttgc tctgcttgcc gcgcgtcagg ctggcgtcaa
     9541 ccgagagggt aaatccgctg tgtgcggcat actgattgag cgctttatcg agcgatcccg
     9601 gtgcgatatt aacctgtgca gcaaaagcgg aaaacgagag accggccagc ggcagcaggc
     9661 tcaggcgaat ggcgttaacc aaaggtgttg ttttacgaaa aacgcgtaac ggcgtcatac
     9721 cttccccatc atcatttttg ttgtgttcag ctatgagtcg aacgagaagg gtaaaaagga
     9781 caatcgaaat aagaattatt ttccttacag tggtgaaatg tttatccagt accgcgtaat
     9841 agactgaatt ttaacgggaa gcgtttgcgc gataacgttc aggatcgcat cggtattttt
     9901 cagcgggaac gtcccgctca ggcgcagccc ggcaacggcg ggatcgcagc gcagcacgcc
     9961 gttgcggtaa cgggttagcg tggctatcac ctcacccagc ggtttatcgc tgaagctcag
    10021 gatgtccttc gtccagcttg tactctcgtc atccagcggt ttcactgcgc caaactcaga
    10081 ggcgctgaac tgcaggcttt caccagcgtt cacgatgcgt ttttgcgcgg gggcactggc
    10141 gagaagcact tccacagcgt gctgctgaac gtcaagctgc gtgaaattat cctgctggcg
    10201 gacggtaaat tctgtcccta aagcagtgag ctggccctga cgggtcagga cacggaaggg
    10261 gcgttgcagg gcatctttcg cggtagtaat ggcgatttca ccgtaccaga gccggacggt
    10321 gcgctgatgc gcatcaaaac gcacatccgc cgcgctttgg gtattcagcg tgagcaggga
    10381 gccatcttcc agttgctgac ggctgactgt gcctttggcg gtgcggtaat ctgcccgcag
    10441 accttcgccg gtttccgact gccagagctg ccagcctccg ccagcgccga gcaacagcag
    10501 taatcctttc atcacgtgac ggcgggtgag gcgggtatcg tgcaacgccc ggctggcaac
    10561 gtcgccaggc acaccgccaa gctggttgcg caggttttca acctgctgcc aggcccactg
    10621 gttatcctga tcctgttcat accactgttg ccagcgcgct tcctgttgtg ggctgacgcg
    10681 ctcgccgctt agcacggcat accagtggga agctgaacgc agcgcctgac ggcgggaatc
    10741 ggttaacaaa ggattcataa cccatactcc agacggaaca gcaggcagtg ctcgacggct
    10801 ttcgccacgt attttttcac ggagctgatg gaaacaccga gtttgtgcgc aatctcgctg
    10861 tatgtcagac catccagttg cgaaagcaga aacgcttcac gtgttttgcc gtttagcccg
    10921 tccagcatgc tgtcgaggag ttgtagggtc tcgagttggc tttcgcgttc ctcaggtgaa
    10981 ggcgctcccc cctccggcat aagcgccagc atctccagat acgctttttc cagcgcgttt
    11041 cggcgaaaca ggtccaccat cacgcgtttg gcgatagtgc agaggaagga gcgaggatcg
    11101 cggatcgtcg agagcgtttc gctgaccatt acccgcaaaa aagtgtcctg ggcaatgtca
    11161 tctgcatcaa aagcagactg gagtttgcgc gtcagccagc ttttcaacca gccgtgatgt
    11221 gtgccataaa gcgactcgaa cgttaaggaa gctgtggtag tggcgcggtc agacatgcgg
    11281 agtgcatcaa aagttaatta tcacgtagtc atattaatat gagaatggtt atcattacaa
    11341 ttggaaataa aattgtttcc aatagacatt tttaacatgt tgtttttcta agtgttataa
    11401 ggtaggtata aaatgggatg gagcctctgc ttctgacatg tgtcggtcag aatgactcat
    11461 gatgtggtct gctattattg acatcctcac tgccctaaag gatggggatt tcggtaatga
    11521 tcttacccag caatagtgga cacgcggcta agtgagtaaa ctctcagtca gaggtgactc
    11581 acaagacaaa acagtttcaa ccagtaaaaa aacgtaaaca ccattcgcat gaatttcgta
    11641 gttaagatct gaagcttgca gaatgcatcg gtgttactgc cggagtccgt gaacttagtc
    11701 tgcatgaatc accacagctt tacatctgtc gcagtaaaca gcaaaatcag caaacttact
    11761 taattatatc gagtgcgatt acaatcgctg gcgatggtac agttggtgtg gcggtctcag
    11821 tccggaacaa tttgaaaatc ataatctcac ttaaaacggc atccatttta caaaagatgg
    11881 ccgtctgtgt atttgtaatg aataccttct acatgtttag agtgcataat ccacgacaag
    11941 gcgatatcct aaaaattagc tagcaggcaa ctttgatggc atagataatg tttttagtaa
    12001 aaaagagttg actgaatata atacagcctc ttcctacata agaaggaatg tgctcacaaa
    12061 aaaaaaccat tagtctgctt cctgttctta caataatcag tattatgtat tttatttata
    12121 tagcaaaata tgcttgttac acctgtcata attaggcatg gtaaaattat tgactgaacc
    12181 ctgggggaga aagatgcaca gtgattatta taatatggtt tttggtgaaa aattggccaa
    12241 tattttatat gaagctaact ctcaattttt ttacgagaga aatgtcatag aagaggcagt
    12301 caacgcttta ttctgtgagc gtgaaataat aaacaacaag aacataataa agaagctgat
    12361 gttctttttg tcagatgtta atcacaccaa gaaagatgtt gttcagagcg cacttaatat
    12421 tatcattgac attacatcag gtgatatttg agattttaca taaaagataa tctctcaagg
    12481 agtcttatct gaaaacgaaa tatatagtcc ctctcgtttt gtaatatcga ttggctttgt
    12541 tgaatcggtt acgtaccccg gagccggtca tattttcagg taatacacac gctttcagac
    12601 atttcggctt tcgtcatttt tgtatcatgg gcatagcttc tgctacctga ccatcataat
    12661 cacgcagctt cagtgaagca gtgaccacct gtttaacccg gtgtatcgtt atgatcttat
    12721 ccagcatgaa ccgccccggg aatcctggag actaaacttc ctgagaaaga ggtaaacagg
    12781 atgactaaaa atactcgttt ttcccccgaa gtccgtcaac gggcagtccg tatggttctg
    12841 gaaagtcaga gcgaatatga ctcacaatgg gcgacaattt gttccattgc tccaaagatt
    12901 ggctgtacgc cggagactct gcgtgtctgg gttcgccagc atgagcggga taccgggggc
    12961 ggtgatggag ggctcaccac cgctgaacgt cagcgtctga aagagctgga
//
LOCUS       DAETPZ010000104        12829 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.104, whole genome shotgun sequence.
ACCESSION   DAETPZ010000104 DAETPZ010000000
VERSION     DAETPZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.104"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(101..739)
                     /gene="rutR"
                     /locus_tag="K0W59_003393"
     CDS             complement(101..739)
                     /gene="rutR"
                     /locus_tag="K0W59_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HBI3024298.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1027..2118
                     /gene="rutA"
                     /locus_tag="K0W59_003394"
     CDS             1027..2118
                     /gene="rutA"
                     /locus_tag="K0W59_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HBI3024299.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPSAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2118..2810
                     /gene="rutB"
                     /locus_tag="K0W59_003395"
     CDS             2118..2810
                     /gene="rutB"
                     /locus_tag="K0W59_003395"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HBI3024300.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGDYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIHHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2822..3208
                     /gene="rutC"
                     /locus_tag="K0W59_003396"
     CDS             2822..3208
                     /gene="rutC"
                     /locus_tag="K0W59_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HBI3024301.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGLVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3216..4016
                     /gene="rutD"
                     /locus_tag="K0W59_003397"
     CDS             3216..4016
                     /gene="rutD"
                     /locus_tag="K0W59_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HBI3024302.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAVRAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4026..4616
                     /locus_tag="K0W59_003398"
     CDS             4026..4616
                     /locus_tag="K0W59_003398"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HBI3024303.1"
                     /translation="MNEAVSPGVLSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4627..5121
                     /gene="rutF"
                     /locus_tag="K0W59_003399"
     CDS             4627..5121
                     /gene="rutF"
                     /locus_tag="K0W59_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HBI3024304.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5142..6470
                     /gene="rutG"
                     /locus_tag="K0W59_003400"
     CDS             5142..6470
                     /gene="rutG"
                     /locus_tag="K0W59_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HBI3024305.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6552..6659)
                     /locus_tag="K0W59_003401"
     CDS             complement(6552..6659)
                     /locus_tag="K0W59_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024306.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7032..7628
                     /gene="wrbA"
                     /locus_tag="K0W59_003402"
     CDS             7032..7628
                     /gene="wrbA"
                     /locus_tag="K0W59_003402"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBI3024307.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7649..7876
                     /locus_tag="K0W59_003403"
     CDS             7649..7876
                     /locus_tag="K0W59_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HBI3024308.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7914..9155)
                     /gene="agp"
                     /locus_tag="K0W59_003404"
     CDS             complement(7914..9155)
                     /gene="agp"
                     /locus_tag="K0W59_003404"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HBI3024309.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9448..10704)
                     /locus_tag="K0W59_003405"
     CDS             complement(9448..10704)
                     /locus_tag="K0W59_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HBI3024310.1"
                     /translation="MGRNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPRISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10965..11885
                     /gene="cbpA"
                     /locus_tag="K0W59_003406"
     CDS             10965..11885
                     /gene="cbpA"
                     /locus_tag="K0W59_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HBI3024311.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFFEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11885..12190
                     /gene="cbpM"
                     /locus_tag="K0W59_003407"
     CDS             11885..12190
                     /gene="cbpM"
                     /locus_tag="K0W59_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HBI3024312.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12283..12699)
                     /gene="torD"
                     /locus_tag="K0W59_003408"
                     /pseudo
     CDS             complement(12283..12699)
                     /gene="torD"
                     /locus_tag="K0W59_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
     gene            12756..>12829
                     /locus_tag="K0W59_003409"
     CDS             12756..>12829
                     /locus_tag="K0W59_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3024313.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTA"
BASE COUNT         3064 a         3267 c         3232 g         3266 t
ORIGIN      
        1 ttcgctgagt gtgaataccg ctgccgctgg cggtaacgcc agtttaatga cgatcggtta
       61 aacaggtagc cggaggatgt tacaacctcc tccggcatct ttaacgtggt cgaatcccct
      121 caataataat ccgctgcacg ttttcaaccg tttgattgaa aaatacctca tcgcgcaacg
      181 tcgcgcctgt caccgcctcc acctgagggg cgaaatcggc gtaatgttga gtggaagccc
      241 aaatcataaa aatcaaatgc tgcggatcaa tcggcgcgag tttgccgctt ttgacccaac
      301 cggcaatcag cgccgatttc tcatcaatta atgccttcaa atcgcccgtc agttcatcca
      361 ttaacaacgg cgcgcctgcc agcatctcca tacagaacag gcgcgaagcc tgcggataat
      421 cgcgtgagac ttccagcttc agacggatgt actcttttat cgccgccagc ggggcgaaat
      481 cttcacgaaa cgcttttaac ggtgccagcc agatatcgag aatctgccgc agcacggcaa
      541 tatacagcgc ctcttttgac gggaagtaat acagcagatt ggtttttgaa acacccgcca
      601 actctgcgat ctgctccagc cttgtgccgt gaaaaccgaa ttgtgaaaaa gtgtccagtg
      661 ctgcgctaag aatcgctttt ttcttcgcgc ttactgcgcg cgaacgttta cccgttgttt
      721 tcactgcgcc ttgcgtcatg cgctctcccc tcttgtagat tggtaacagg atagcaaaac
      781 ccccgcccgg cctcgacctt ctgcactctc atcgcgcacg gatgcatgtt ttatgtgcaa
      841 ctgttttgac cgtttagtcc actttttacc agatatttta gataacacaa tgctaacaca
      901 ttaataacat tcacttttta aaaactggca tccgctttgc aaacaagcca atacgcggct
      961 gaaaaggata tgcaggatgc agcgccccgt ctgactttca ctttacgaga cgaagagagg
     1021 ttgatgatga aaattggcgt attcgtacct attggcaaca acggctggct catttcgacc
     1081 cacgcgccgc agtacatgcc gacctttgaa ctgaataaag ccattgtgca aaaagcggag
     1141 cattaccatt tcgatttcgc cctgtcgatg atcaaactgc gtggctttgg cggcaaaact
     1201 gagttctggg atcacaacct tgagtcgttc accttgatgg cgggtctggc agccgtgacc
     1261 tcgcgcattc agatttacgc cactgctgcc accttaacgt tacctccggc aatcgtcgcc
     1321 cgtatggccg caaccatcga ctccatctct ggcgggcgtt ttggcgtcaa cctcgtgact
     1381 ggctggcaaa agcccgagta tgagcagatg ggtatctggc ctggcgatga ctatttctcc
     1441 cgtcgttacg actatctcac cgaatatgtt caggtgctgc gcgacctgtg gggcacgggg
     1501 aaaagcgatt ttaaaggcga ttttttcacc atgaatgatt gtcgcgtcag tccgcaaccg
     1561 agtatcccca tgaaagtgat ctgcgccggg caaagcgacg ctggcatggc gttctccgcc
     1621 cagtatgccg atttcaactt ctgtttcggc aaaggcgtaa atacacccac ggctttcgcc
     1681 ccgaccgctg cgcggatgaa acaggccgca gagcaaaccg gacgcgacgt tggctcttat
     1741 gtgttgttta tggtgattgc cgatgaaacc gacgatgcca ctcgcgccaa atgggaacac
     1801 tacaaagcgg gcgcggatga agaggcgtta agctggctaa ccgaacaaag tcagaaagat
     1861 acccgctccg gtactgacac caacgttcgt cagatggccg atcccacttc ggcggtaaac
     1921 atcaatatgg ggacgttagt cggttcttac gccagtgtcg cgcgcatgtt agatgaagtc
     1981 gcaagcgtgc ctagtgcaga aggcgtgctg ttgaccttcg atgattttct gtcgggaatc
     2041 gaaaccttcg gcgagcgcat tcaaccactg atgcagtgcc gcgcccatct ctctgcgctg
     2101 actcaggagg tggcatgatg acaaccttaa ccgctcgacc ggaagccatt accttcgatc
     2161 cgcagcaaag tgcgcagatc gtggtggata tgcaaaacgc ttatgccacg ccaggcgact
     2221 acttagatct cgccgggttt gatgtctcaa ccactcgccc ggtcattgcc aacattcaaa
     2281 ccgctgtgac cgcagcgcga gcggcaggga tgttgatcat ctggtttcaa aatggctggg
     2341 atgaacagta tgtcgaggct ggcggccccg gctcaccgaa ttttcataaa tcgaacgccc
     2401 tgaaaaccat gcgtaagcag ccgcagctgc aggggaaatt gctggcgaaa ggctcctggg
     2461 attatcaact ggtggatgaa ctggtgccgc agcctggcga tattgtgctg ccgaagccgc
     2521 gctacagcgg tttcttcaat acgccgctgg acagcatttt gcgcagccgt ggaatacacc
     2581 atctggtttt caccggtatc gctaccaacg tctgcgtcga atcgacgcta cgcgatggct
     2641 tttttctgga gcatttcggc gtggtgctgg aagacgcaac acaccaggcg gggccggaat
     2701 ttgcacagaa agccgcgttg ttcaatatcg aaaccttttt tggctgggtc agcgacgtcg
     2761 aaacgttctg cgacgcgctt tctcccacgt cctttgctcg tatcgcttaa ggagtttaac
     2821 gatgccaaaa tccgtaatta ttcccgctgg cagcagcgca ccgctggccc ccttcgttcc
     2881 cggcacgctg gctgatggct tggtgtatgt ctccggtacg ctggcttttg atcaacataa
     2941 taacgtgctg tttgccgatg acccaaaggc gcaaacccgc cacgttctgg aaactatccg
     3001 caaggtgatc gagacggcgg gtggcacgat ggcggatgtg accttcaaca gcatctttat
     3061 taccgactgg aaaaattacg ccgcgattaa cgaaatctac gccgagtttt ttccgggtga
     3121 taaaccggcg cgattctaca ttcagtgcgg actggtaaaa cctgacgcgc tggtggaaat
     3181 cgccacaatt gcgcatatcg ccaagtgagg ccgcgatgaa actttcactc tcacctcccc
     3241 cttatgctga tgcacccgta gtggtgttga tttcgggtct tggcggtagc ggcagttact
     3301 ggttaccgca actggcggtg ctggagcagg agtatcaggt agtctgttac gaccagcgcg
     3361 gcaccggcaa taatcccgac acactggcag aagattacag tatcgcccag atggcagcgg
     3421 aactgcatca ggcgctggta gccgcaggga ttgagcgtta cgcggtggtc ggccatgcgc
     3481 tcggtgcgct ggtggggatg cagctggcgc tggattatcc cgcgtcggta actatgctgg
     3541 tcagcgttaa cggctggcta cgaataaacg cccatacgcg ccgctgtttt caggttcgcg
     3601 aacgattact gtatagcggc ggcgcgcaag catgggtgga agcgcagccg ttgttcctct
     3661 atcccgccga ctggatggcg gtccgcgcac ctcgcctgga ggcagaagac gcgctggcac
     3721 tggcgcattt tcagggcaaa aataatttac tacgtcgact taacgccctc aaacgcgctg
     3781 actttagtca ccatgcggat cgcatccgct gcccggtgca aatcatctgc gccagtgatg
     3841 atctgctggt gccaacagca tgttccagtg aacttcatgc cgccctgccc gatagccaga
     3901 aaatggtgat gccctatggc ggacacgcct gcaacgtgac cgatcccgaa acgtttaatg
     3961 ctctgttact caacgggctt gccagcctgt tacatcaccg tgaagccgcc ctgtaaggaa
     4021 ttgctatgaa cgaagccgtt agcccaggtg tgcttagcac cctgttcacc gatgcccgca
     4081 ctcacaacgg ctggcgggag acacccgtca gcgatgagac gttacaggag ctttatgccc
     4141 tgatgaaatg ggggccgaca tcagctaact gttctccggc acggatcgtg tttatccgca
     4201 ctgcagaagg aaaagaacgt ctgcgcccgg cgctttccag cggcaatctg caaaaaaccc
     4261 tgaccgcgcc cgtcaccgct atcgtcgctt gggacagtga attttatgaa cggttaccac
     4321 tactgtttcc ccacggtgat gcccgcagtt ggtttacctc cagcccacaa cttgccgaag
     4381 aaacagcgtt tcgcaacagt tccatgcagg cggcctatct gatcgtcgct tgccgtgcgc
     4441 tgggactgga taccggcccg atgtcgggtt ttgaccgtca acacgttgac gacgcctttt
     4501 ttgcgggcag cacgctgaag agcaatctgc tgattaatat cggctatggc gatagcagca
     4561 aactttttgc gcgcctgcca cgtctgtcct ttgaagaagc ctgcgggctg ttgtaaggag
     4621 caaactatga acatagttga tcaacaaacc tttcgcgatg cgatgtcctg tatgggcgcg
     4681 gcggtcaata tcatcaccac ggacggtcca gccgggcgcg ccgggttcac cgccagcgcc
     4741 gtctgcagcg tgaccgacac gccgcccaca ttactggtgt gcctgaatcg tggggcgtcc
     4801 gtctggccgg tattcaatga aaatcgaacg ctgtgtgtaa atacactgag cgccgggcag
     4861 gagccgcttt caaatctttt tggcggcaaa acgcccatgg aacaccgctt tgccgccgcc
     4921 cgctggcaga cgggtgtgac cggatgcccg caactggaag aggctctggt ttcgtttgac
     4981 tgccgtatca gccaggtggt gagcgtcggc acccacgaca ttctgttttg cgccatcgaa
     5041 gcgattcatc gtcacaccac accctacggg ctggtgtggt tcgatcgcag ttatcacgcg
     5101 ctgatgcgcc ctgcttgtta acctccttaa ggagacagct gatggcaatg ttcggttttc
     5161 ctcactggca gttaaaatcg acctctacag agagcggcgt ggtcgcgccg gatgaacgat
     5221 tgccgtttgc tcagacggca gtaatgggcg ttcaacacgc ggtggcgatg tttggcgcga
     5281 cggtgttaat gccgattctg atggggctgg atcccaatct ttccatttta atgtcgggga
     5341 tcggcacgtt actgtttttc tttattaccg gcgggcgcgt ccccagttat ctcggctcca
     5401 gcgcggcttt tgtcggcgtg gtgattgccg cgacgggctt taacggtcag ggcattaacc
     5461 cgaatatcag catcgcgctg ggcgggatta tcgcctgtgg gctggtttat accgtcattg
     5521 gcctggtggt gatgaaaatt ggtacacgct ggattgaacg actaatgccg ccggtggtga
     5581 cgggtgcggt ggtgatggcg attggcctga acttagcgcc tatcgcagtg aaaagtgtat
     5641 cggcttcggc ctttgatagc tggatggcgg taatgacggt gctgtgtatt gggctggtgg
     5701 cggtgtttac ccgtggcatg atccagcggt tattgattct cgtaggatta attgtcgcct
     5761 gtctgctgta tggcgtgatg accaacgttt tagggctggg aaaagccgtc gattttactc
     5821 tcgtcagcta tgctgcctgg ttcggtctgc cgcatttttc cactcctggt tttaatggcc
     5881 aggcgatgat gttgattgcg cccgtggcgg tgattctggt ggcggaaaac ttaggtcatc
     5941 tcaaggcggt cgccgggatg accggacgca atatggaccc gtatatgggg cgagcattcg
     6001 tcggtgatgg tctggcaacg atgctttccg gctctgtcgg cggtagcggc gtcaccacct
     6061 atgctgaaaa tatcggtgtg atggccgtga caaaagtcta ctcgacgctg gtgtttgttg
     6121 cagctgccgt catcgctatg ctgttgggct tttcgccgaa atttggcgcg ctgatccata
     6181 ccattccggc cgcggtgatt ggtggtgcat caattgttgt gttcgggctg attgccgtcg
     6241 caggcgcaag gatatgggta caaaaccgtg tcgatttaag ccagaacggt aatttgatta
     6301 tggtcgcagt aaccttcgtt ctgggcgcgg gtgattttgc tctcacgctt ggcggtttta
     6361 cgttgggagg gatcggtaca gcaacctttg gcgcgatttt actcaatgcg ttattaagca
     6421 gaaggttggt tgacgttcca ccgccagaag ttgttcatca ggagccataa acagaaaacc
     6481 gccgggttac cggcggtgat gactttttga cgagcttaag aaaaaaatgc attcgtcatt
     6541 gattagcccg gctagttgtc gcttttgccg tgactgctct tgccaccttt tttacctgct
     6601 tctgatgctc tttcgcggtc ttctgcaaaa ttgccggaac cgcctcgatg gtttgccatt
     6661 actgacctcc gtctgggtat agacatcata ttgcatagaa ttaacgcggc gtatggcggc
     6721 tgccatattt tcgctacgtg ggactaagct tagtgaactg acgcgggcag acccgcgttt
     6781 ggtaatatcc tgcaacagct tcttagcgag aatagatatc atctccaata attatccata
     6841 agccgctctt atgattaaaa aaacgcgatg agataaaaat gtgtcatttt gcgacaaaat
     6901 tacgtgcttg tgaaagttgt tataaatcaa ataagtggtt gtgaaatttg cactctgaaa
     6961 aggacgtctt atctttaaat aagtggtagc gaatcgctac ggaatagaga taacacgagg
     7021 agtggttaga aatggctaaa gttctggtgc tttattattc catgtacgga catattgaaa
     7081 cgatggcacg cgcagtcgct gagggtgcaa gcaaagtgga tggtgcagaa gttgtcgtta
     7141 agcgtgtacc ggaaaccatg ccgccgcaat tatttgaaaa agcaggcggt aaaacgcaaa
     7201 ctgcaccggt tgcaaccccg caagaactgg ccgattacga cgccattatt tttggtacac
     7261 ctacccgctt tggcaacatg tccggtcaaa tgcgtacctt cctcgaccag acgggcggcc
     7321 tgtgggcttc cggcgcacta tacggaaaac tggcgagcgt ctttagttcc accggtactg
     7381 gcggcggtca ggaacaaacc atcacatcca cctggacgac ccttgcgcat cacggcatgg
     7441 tgattgtccc cattggctac gcagcgcagg aattatttga cgtttcacag gttcgcggcg
     7501 gtacgccgta cggcgcaacc accatcgcag gcggtgacgg ctcacgccag ccaagccagg
     7561 aagaactgtc tattgctcgt tatcaagggg aatatgtcgc aggtctggca gttaaactta
     7621 acggctaatc ttcaacagga ggatacgcat gccaactcaa gaagcgaaag ctcatcacgt
     7681 cggtgaatgg gcatctctgc gcaatacgtc gccggaaata gccgaggcca ttttcgaagt
     7741 cgccgggtac gacgaaaaaa tggcagaaaa gatttgggaa gaaggtagtg atgaagtgtt
     7801 agtcaaagcc tttgctaaga ccgataaaga ttcgcttttt tggggcgaac agaccatcga
     7861 acgtaaaaac gtttaaccag cgactccccc gcttctcgcg ggggagtttt ctgttatttc
     7921 accgcctcat tcaacacgct atcaaactta tccatcgggc agaaaccgtt ggcatctatc
     7981 gggcaaccgc ttaattccag cgtcacgcgc tgcgcaggtg cctgcagggt taacgcatcg
     8041 gcattacgta actgttccgc actctggtac acatattcaa ttttcatcaa atcgcgattg
     8101 gctttgctgt catgccaacg ctggaaaacg attttgccgc caatcggcgt gcgttcgttc
     8161 tggtcatgca actgatacgg tttgaaatcc agcgccgtta acagagaggc tatgttggag
     8221 tcgtgcccaa ctaacaccgt gattttcgga gcactggcgc gatcggtgac cagggctttg
     8281 tcgatataac tgaccagcgg tttcgcaaca ttgcgcgcca cttccggtga ggtaaacaga
     8341 ctgtcctggt aaccgttttt cagcttcgac aataccttcc actgctggtc agatttgatt
     8401 tctccccagg ccacctgatc catcggaaaa ccttcgtagt attgcaaagt aaacgcatct
     8461 accagcgagt tgccgacttt cagcggcccg gaaacacctg gttcttgttg atacttggcg
     8521 ctaaaggtat ttttgccatc caccagcgaa cactgttgtt tctctttaca ggcaggggaa
     8581 tctttatagt taacgatttt ttccagtagc tggtagctgt cggtaagctg gagtttgctg
     8641 agctctttct ccattgccgc caccgcctgt tcactgaatg cggcggaatc atcggtgatc
     8701 atcgggttaa aggttgggtc catagtgccc attttttcct ggtgatgcac aggaatatca
     8761 cacccaggga atgcaccagt aataaagaac tgggcggtag cgacggtacg ttgcagacta
     8821 ttggcatagg cataaacggt gtccggcggc gggcattccc ccgatttcac tatcccctgc
     8881 tctgccagcc attcacgcat gtaatggccc atatacactt cgagcacgcc acctttggtg
     8941 gtgagttgcc caccggggac gtcccattcg ggccatttat tcggcgtcga ctgctccagc
     9001 acactgccat tgttcgccag cggcgcacgt aagttatggc ggctcatcat gagcacttgc
     9061 tgtagctgat agccttccgg tacggtttgt gcctgagcat ttgaagcgag taaaactatc
     9121 cctgccacag ttgcggcgat tagcgttttg ttcattcctg gcacctcttt tgttatcaat
     9181 cattaaagag tgtgacagaa atatgacttt tttccgggaa caggttcgca aaatggcttg
     9241 ttgaacgcct gtttttctat atacatcttc aaaaacaggc aaggtttgac gcaaactatg
     9301 cctgattagc ccctgcctcg cgtacgccgc tgataattgc acctgtggct aaacagaata
     9361 tctgcggcgt taccaataca gagacaatat ttaccaataa aaaagtgtga gactaataac
     9421 aatggttttt agcatatgcc tttgccatta agttgcataa cgatttaaat agtcaagttg
     9481 cgcattcatt tccttatttt ttaacttata agcctgagaa aaaagttcag gagggaggta
     9541 aacatcataa tgattgccag tgtttattaa ataaccatac tcaagatctt catctgttga
     9601 aaaggattca tttcccaagg agattgatgc cacattacta ttaacaataa ataatgagca
     9661 aatgctatct gaaagaacag caagaaaatt cttcaccgct ttaatcctat ccgacgtgtc
     9721 ggtatctaaa gcaagcaaat tcttgttatc attaaatata aactcgggta aatgcagtat
     9781 gcattttttg tcattcaagc aactatcaat gaattgtttt ttaaaaaaca gaaagctcca
     9841 tgacataaaa ctacccacac cctgaatttt gttgtcagat tgcagatcaa cgccaaataa
     9901 tgtctttaca tcttccctga gattatagaa tgccgtatca acaagagcct cgatcgtttg
     9961 tttatcaaca attttaataa actcttcgta tacttgcttt tgcgtcttat ggctgcccag
    10021 cgcccatgaa tactcatgtt cttttaaaac cagtatggct cttaacagac aatatccatc
    10081 gccatctact ggagcgtatt ttaatccata tgttttatta atttcactct gaataaaaga
    10141 tatgccagac aaatcaatac cagtatctaa atcagatatt ctcggcatat ttatctgttc
    10201 tggctggata ctcatatttt catcatctaa acaatactca gaaaaaacat catcacacgc
    10261 attaatgttt tcaatatatt ccataaagcc ataccaataa taacctttaa aatctgtgat
    10321 agactctgaa cctctgcccg gcaaggacac tctaacaata tcaacatctt gaacatattg
    10381 tatagtatat gtaatacctc ctgaaacccc tgaataaatt tgtgtatcat tagtacgcaa
    10441 tgttctcata aataatgata gatcattatc attctgcatt tcctcaaatt ctttttctag
    10501 tgattcacat tccagacatg gagtatcctc ttcatttact ttacgtctga gttcattgta
    10561 caaccgcata aaacacacca gtgtgttttc aaaaagcgtg attgaatatg tttgattttt
    10621 ttcgtactca ttttttatat cgttccaggc ctgtttcaaa taattatttg cagtgcaact
    10681 aattccatgt atattacgac ccatatatag cgtctataaa atttaataaa taatgacgcc
    10741 ctagttaaac ttaaagtgcc tggttcaact atcaaaaatc gctcacccgt tttcacctgt
    10801 ttaaaatatg ttcagcaacc catcttgatg gcgacctcct ctccgcgatg atttcaataa
    10861 catattctgt gttggcatat gaaattttga ggattaccct acacttatag gagttacctt
    10921 acaggggttc cttcaatttg tgttgattta cgcgagataa cgctatggaa ttaaaggatt
    10981 attacgccat catgggcgtg aaaccgacgg acgatctcaa gacaatcaag accgcctatc
    11041 gtcgactggc ccgtaaatac catcctgatg tcagcaaaga accggatgcc gaagcccgct
    11101 tcaaagaggt cgctgaagcc tgggaagtgt taagtgatga acaacgtcgc gctgagtatg
    11161 atcagatgtg gcaacatcgc aacgatccgc aatttaaccg tcagttccat catggcgacg
    11221 gtcagagttt taatgccgaa gattttgacg atatcttctc gtcaattttc ggtcagcatg
    11281 cccgccagag ccgtcaacgc cccgccgcac gcggccacga tattgaaatc gaagtggcgg
    11341 tattcttcga agaaacgctt actgagcata agcgtaccat cagctataac ctgccggttt
    11401 ataacgcctt tggcatgatc gaacaggaaa tcccgaaaac gctgaatgtg aagatcccgg
    11461 caggcgtcgg caatggtcaa cgcatccgcc tgaaaggcca ggggacgccg ggcgaaaacg
    11521 gcggtccaaa tggcgatttg tggctggtga ttcatattgc gccacatccg ctgttcgata
    11581 ttgtcggcca ggatctggaa attgtggtgc cggttagccc gtgggaagcg gcgctgggta
    11641 ctaaagtcac cgttccaaca ctgaaagaaa gcattttgct gactatcccg ccaggcagcc
    11701 aggccgggca acgattgcgc gttaaaggca aaggtctggt gagcaaaaaa cagaccggcg
    11761 atctgtatgc ggtactgaaa atcgtgatgc cgccgaaacc ggatgaaaac actgccgcac
    11821 tgtggcagca actggcagac gcccagtcgt cttttgatcc acgtaaagat tgggggaaag
    11881 cataatggct aatgttacgg tgacttttac tattaccgaa ttttgcctgc ataccggcat
    11941 ctctgaagag gagttgaatg aaattgtcgg tttgggggtg gttgaaccgc gtgagatcca
    12001 ggaaacaacc tgggtatttg acgaccatgc cgccattgtg gtgcaacgcg cggtacgcct
    12061 gcgtcatgaa ctggctctgg actggccggg gatcgcggtg gcgctgacgt taatggatga
    12121 tattgcgcac ctgaagcagg aaaaccgcct gctacgccag cggctttccc ggtttgtagc
    12181 tcatccgtga tgtgtgtttg ccggatgtga tgacgcatcc ggcaactgcg ccataatgcc
    12241 tgatgcgacg ctaaacgcgt cttttcaggc gcataaacga cgttatctgt tttggtggtc
    12301 gcactccact aacaccagca ataactggct tagtgccgcg taaaaaccaa agctgtcata
    12361 ctgtcgacaa cgcgcagcaa actctggtaa ccattgccgt agcgccgtca gtgttttttg
    12421 ccgcaaactg tcgattcttc gcgcaggaac ggtcccctct cccagcgaaa aatgcagata
    12481 actcaacaac tcgagataga tcgccagatg atctgccggt tcgttgaaat tgccactggt
    12541 ttccatccct gcctcaacta acaagcgttt aatctcttgc tcgtcctgtt tgtaggccga
    12601 tgcatacggc agcgccgctt gtttgtcggt catcagaaac aggccgcaaa agtcagcggc
    12661 cagttccaga cgggcatcgt cacgtactgt cagcgtggca ggtaatgctg ccaacttact
    12721 gatttagtgt atgatggtga ttttaaggta cttgcgtggc ttccatttcc atcagatgtc
    12781 cttcctgctc cgctactgaa ggcgtggtgc gtaacggcaa aagcactgc
//
LOCUS       DAETPZ010000105        12511 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.105, whole genome shotgun sequence.
ACCESSION   DAETPZ010000105 DAETPZ010000000
VERSION     DAETPZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.105"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            34..435
                     /locus_tag="K0W59_003410"
     CDS             34..435
                     /locus_tag="K0W59_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBI3024314.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVSAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="K0W59_003411"
     CDS             447..818
                     /locus_tag="K0W59_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3024315.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..1395
                     /locus_tag="K0W59_003412"
     CDS             805..1395
                     /locus_tag="K0W59_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3024316.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            1392..1791
                     /locus_tag="K0W59_003413"
                     /pseudo
     CDS             1392..1791
                     /locus_tag="K0W59_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1802..2542
                     /locus_tag="K0W59_003414"
     CDS             1802..2542
                     /locus_tag="K0W59_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HBI3024317.1"
                     /translation="MTTPNPLAKTKGAGTTLWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVPLSGLVATVKG
                     VQQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            2601..2987
                     /locus_tag="K0W59_003415"
     CDS             2601..2987
                     /locus_tag="K0W59_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBI3024318.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRKAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2996..3325
                     /locus_tag="K0W59_003416"
     CDS             2996..3325
                     /locus_tag="K0W59_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBI3024319.1"
                     /translation="MQFVMRLAREFRRADWRQMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            3297..6338
                     /locus_tag="K0W59_003417"
                     /pseudo
     CDS             3297..6338
                     /locus_tag="K0W59_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            6338..6667
                     /locus_tag="K0W59_003418"
     CDS             6338..6667
                     /locus_tag="K0W59_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3024320.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIREVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            6667..7365
                     /locus_tag="K0W59_003419"
     CDS             6667..7365
                     /locus_tag="K0W59_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBI3024321.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGAGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            7370..8113
                     /locus_tag="K0W59_003420"
     CDS             7370..8113
                     /locus_tag="K0W59_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3024322.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            8050..8651
                     /locus_tag="K0W59_003421"
                     /pseudo
     CDS             8050..8651
                     /locus_tag="K0W59_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843089.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            8712..12191
                     /locus_tag="K0W59_003422"
                     /pseudo
     CDS             8712..12191
                     /locus_tag="K0W59_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            12259..>12511
                     /locus_tag="K0W59_003423"
     CDS             12259..>12511
                     /locus_tag="K0W59_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBI3024323.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQPHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSD"
BASE COUNT         2960 a         2953 c         3980 g         2618 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgctca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctctgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccgctgtc cggaccgctg acacaggcat ttgaagatgc ccgcgatcgc
     1321 atcattgctg cggaaatgcc gaaacagctg gggtatgccc tgaaacaaca actgaggtta
     1381 catctgagta aatgaaccgg cacacacaaa tccgtcagag cgtgctggca cgccttcggg
     1441 aacagtgtgg agacagcgcc acgttttttg acgggcttcc ggcatttatt gatgcgcaaa
     1501 ctgcctgccg tggcggtgtg gctgagtgat gctcagtaca ccggaaaaat gacggatgga
     1561 gatgactggc aggctgttct gtatattgcc gtcttcatcc gggcacaggc accggattca
     1621 gagctggata tgtggatgga gagcaccatt ttcccggccc tgaatgatgt accggcactt
     1681 tccggactca tcgacaccct gaacccactc ggttttaact atcaacgtga taatgagatg
     1741 gctacctggg cgatggcgga aatcacgtac cagatcacgt acacgaatta aggaggtggt
     1801 aatgaccaca ccaaatccac tggcaaaaac gaaaggtgcg ggaacgacgt tatggatgta
     1861 taccggcaac ggcgacgcat ttgcgaaccc tttgtcggac actgactggc tgcgtcttgc
     1921 gatggtgaag gatctgcaac ctggcgagat gaccgctgat gcagaagatg acacttatct
     1981 cgatgatgaa gatgcagact ggaaaacgac aacccagggg cagaaatccg tcggtgatac
     2041 ttcggcgacg ctggcctggc gtccgggtga cagcgggcag aaaaaactgg ttcagttgtt
     2101 cgattccggt gaagtctgcg cgtttcgtat caaatacccc aacggtacgg ttgatgtttt
     2161 ccgtggctgg ctgagttcac tgggtaaaac cattacctca aaagaggtga tgacccgtac
     2221 tgtaaaaatc agcggtgtgg ggcgtccgta tctggcagag gagggcgctg aaaccgtggg
     2281 tgttaccgga ctgacggtgg caccggcatc tgtcagtgtc aaagcgggag caaccaccac
     2341 actgaccttt acagtaaaac ctgacggggc cagtgacaaa gcgatcagtg tgcattcgtc
     2401 agatcctcag actgcttcgg tgcccctgag cgggcttgtg gccacggtga aaggcgtgca
     2461 gcagggcagt gtcagcatcg tgggcatgac ctctgacgga gagtttgtgg cagtggctgc
     2521 ggtgaccgtc agcgcaccat aacaggacga tactcatcat tgccccggtt atccggggct
     2581 ttttgcatcc ggagaacatg atgtttctga aacaggatac gtttaattat gaaaaacagt
     2641 ccgtggtgct cagtgagctg tccgggctgc agagaattga atatctgacg tttgttcagc
     2701 agcgaaaggc aaagtttgat gcacaggagg gagaactgcc ggaggctgaa cgacagattg
     2761 cttttctgcg tatgggaatg gatatcaatg cctggctggt ttcccgctca ctgtggaatg
     2821 ctgagcagtc tcaggatgtt gagacgcttt gcgcatccat tatgacaaca tggtcgtatg
     2881 atgcgctggg cgcgggggcg gagagggttc tgtcgctgag cggtatgggg accattgaga
     2941 atgccgggga tgatgatcat gaggcgctga cgccggaaaa gtcctgacgc gggaaatgca
     3001 gtttgtcatg cggcttgccc gggagttccg gcgggcagac tggcggcaga tgctgtcgga
     3061 tatgtcggcc actgagcttg gtgagtgggg cgattatttc cggatgcaga gcttcagtga
     3121 tgtgtggatg gatgcgcagt ttgcctcgct gaaggcattg atcgtgagaa tggtgtccgg
     3181 cagcagcgat gctgcggtgg ctgatttcag ccttttaccg gaagagaacg ggataccgga
     3241 gcgaacggac gaagaactga tgcatcttgg ggaaggtatt tccggaggtg tgcgttatgg
     3301 accagatagc caacctggtc attgatttgg ggattgatgc ggcagagttt aaaaatgaaa
     3361 ttccccgtat caaaaacctt ctgaatggtg cagccagcga tgcagaacgg tcttctgccc
     3421 gtatgcagcg ttttatggag cgtcagactc aggcggcccg gcagacaatg caggcggctt
     3481 cttcggctgc aacagcagca tcagcccatg cgcagacggt ggagaagaat gcacgggctc
     3541 atgaacgcat ggcccgtgag gtggaacaaa cccgtctgcg cgtggatgcc ctgaatcaga
     3601 aaatgcgcga ggaacaggcg caggccaggg cactggcgga ggcgcaggat aaagcggctg
     3661 ccgcctttta tcgccagatt gacagtgtga aacaggccgg tgcggggctt caggaattac
     3721 agcgtattca gcagcagatc cgacaggcca gaaacagtgg cggggttggt cagcaggatt
     3781 atctggcgct gatttcggag atcacggcga aaacccgtgc cctgacgcag gcagaggaac
     3841 aggccacccg gcagaaagcg gcgtttatcc gccagcttaa agagcaggca acccgccaga
     3901 atctgtcgtc ttctgagttg cttcgcgcca gggcggctca gctgggggta agcagtgctg
     3961 cagaagtgta tatccgcaaa atggagcggg caggaaaagc cacacattcg ctggggctga
     4021 aaagtgcggc agcccggcgg gagctggggg tgttaatcag tcagatggcg cgcggcaatt
     4081 ttggtgcgct gaggggatcc gggataacgc tggctaaccg tgccggatgg acaggcgcac
     4141 tgatgtcgcc gaaaggcatg atgactggcg gcgttattgg cggacttgtc gcggcggtcc
     4201 tgggtctggg taaagcctgg catgacggcg gaaggagggc gaggaattta accgtcagct
     4261 ggcgctgacg ggacattatg ccggtgtcac tgtcgggcag ttgtggaaac tcagccgggc
     4321 catatccggg aatggtatca cgcaacatgc ggcagccggt gcgctggcac aggtggtggg
     4381 cagtggggca tttcatggaa acgatattgg tatggtggcg aaagctgccg cacagatgga
     4441 gcgatcggtt ggccagtcgg tcagcgatac cataaatcag tttaagcggc tgaaggatga
     4501 tcctgtaaat gccgcgaagg cgctggacaa tgcgctgcat tttttgactg ccactcagct
     4561 tgagcagata cgcgtccttg gggagcaggg gcggtccagt gatgcggccc ggatcgccat
     4621 gtctgcgctg gcagaggaaa ccggtaaacg cacatctgat attgataata atctcaatgc
     4681 gctgggtagt acgctgcaaa ccttgtctga ctggtggaag cagttctggg atgcggccat
     4741 gaatattggt cgtgaagact cgctggatgc gcagattgat gcgttacagg aaaaaattca
     4801 gcgcgcgaaa aaatatccgt ggacaaacgc ctccacacag gtggagtacg atcagcagcg
     4861 tcttaacgat cttcaggaga aaaaacgccg gaaggatttg caggatgcaa aagcgcaggc
     4921 agaacggaat taccaggagc aacagaaacg ccggaatgct gaaaatgccg cgctgaaccg
     4981 gatgaatgaa acggaagctg cacgacatca gcgggaaatt gcgcgtatta atgccatgca
     5041 gtacgccgac caggctgtca gggatgcggc gatacagcgt gaaaatgaac gttacgaaaa
     5101 agccattaag aaaaatacac gggcaacccg taatgatgag gccacccggt tattgctgca
     5161 gtacagtcag cagcaggcac aggtggaagg gcagattgct gctgccagac agtcagcagg
     5221 cattgccact gaaaggatga ctgaagcgca taaacagctt ctggccctgc agcagcgcat
     5281 cagcgacctg gacgggaaaa agctgacggc agatgaaaag agtgtgctgg cccgtaagaa
     5341 tgagctgatt caggcgctga cgctgctgga tgtgaaacag caggagctgc agaaacagac
     5401 agcgcttaac gacctgagaa aaaaaacggt tcagctgacc agccagctgg cagacaaaga
     5461 acgtgcactg cgtgagcagc acaatctgga tattgccact gcaggtatgg gggataagca
     5521 gcggcagcgc taccaggcac agttgcgcat ccggcaggaa taccggcaac agttgcaaca
     5581 gcttgagaat gacagtcgcc agaaaggcac ttacgggacg gaggactacc ggagggctga
     5641 ggaggtgctg aaggggagcc tgaagcgaca actgaatgaa aacaaacgct actggcagga
     5701 actggaagtg gcgcagggcg actggaaaaa cggtgccatg cgggcgtttc agaattttac
     5761 ggcggatgcg gataatgcgg cgggaacggc agaacagatg tttacagtgg cattcagcag
     5821 tgccggtaat gcactggcga cattctgtac caccggtaag ctgaatttta aatccttcac
     5881 ctcttccctg ttgtcagata tggccagaat tatggcacag atggccatga tgcaggcggt
     5941 aaagggcgtc ggttctttat tcggcttcac gactaatgct gatggcggtg tttaccagtc
     6001 tgctgatttg agtcgctaca gtggcacggt ggttaaccgt ccgacgtttt ttgcttttgc
     6061 aaaaggcgcg ggtgtgatgg gggaagcggg acctgaagcc attctgccac tgcgtcgcgg
     6121 tgctgatggt aagctggggg gttgtggcgg atactggtgg ttcaggcatg gtgatgtttg
     6181 ccccgcagta caacattgag atcaataacg acggcacgaa cgggcagata ggtccggctg
     6241 ccctgaaggt ggtttatgac ctcgggaaaa aagcggccgc ggactttatg caacagcagg
     6301 cccgtgatgg tggccggtta agtggagcat accggtaatg gagacgtttc actggaaagt
     6361 gcgcccggat atgaatgtgg tatcagagcc gaaagtggtg acagtgaagc tgggcgatgg
     6421 ttatgaacag cgtcgtgcgg cgggactgaa taaccagttg tcgacttaca gcgtgacgat
     6481 acgtgttcgt aaaggtgaac acccatcttt aaaagccttt ctggaacggc acggtggcgt
     6541 ccgcgcattt cagtggacgc caccttatga ctggaaactg atcagggagg tttgtcgtaa
     6601 atggtcggca agcgtggggg cgttatgggt gactgtaacg gccgattttg aacaggtggt
     6661 gaactgatgc aggatatccg gcaggaaaca ctgaatgaat gcacccgtgc ggagcagtca
     6721 gcccgggtgg agctctggga aatcgatctg acagaggtcg gtggtgagcg ttattttttc
     6781 tgtaatgagc agaacgaaaa aggtgagccg gtcacctggc aggggcggca gtatcaggca
     6841 taccccattc agggggcggg gtttgaactg aacggcaggg gctgtgctgc ccgtccgaca
     6901 ctgacggtct ctaacctgca cggcatggtc accgggatgg cggaagatct gcagagtctg
     6961 gtcggcggaa cggtggtccg gcgtaaggtt tacgcccgtt ttctggatgc ggtgaacttc
     7021 gtcaacggaa acagcgacgc cgatccggaa caggaggtga tcagccgctg gcgcatcgag
     7081 cagtgcagcg aactgagcgc ggtcagtgcc tcttttgtgc tggccacacc aacggagacg
     7141 gatggcgcgg ttttcccggg gcgtatcatg ctggcgaata cctgtatgtg gacttaccgt
     7201 tctgatgagt gtggttacac gggcagggca gtggctgacg agttcgacaa accaacgacg
     7261 gatatccgga aggacaaatg cagcaagtgt atgcgcgggt gtgagttgcg caacaatacc
     7321 ggtaatttcg gcggtttcct ttccatcaat aaactttctc agtaaatcca tgacacagac
     7381 agaatcagcg attctggcgc acgcccggcg atgtgcgcca gcggagtcgt gtggattcgt
     7441 ggtgagaacg ccggagggag acaggtatct tcccagcgag aatatctccg gtgagccgga
     7501 ggaacggttc cggatggctc cggaggactg gctgcgggca caaatgcagg gtgagattgt
     7561 ggcactggtc cacagtcatc ccggtggtct gccctggctg agtgaggctg accggcggct
     7621 gcaggtgcag agtgatttgc cgtggtggct ggtctgccgg ggggcgattc acaagttccg
     7681 ctgtgtgccg catctcaccg ggcggcgctt tgagcacggg gtgacggact gttacacgct
     7741 gttccgggat gcttatcatc tggcggggat tgagatgccg gattttcacc gcggggatga
     7801 ctggtggcgt cacggtcaga atctctatct ggataatctg gaggccacag ggctgtatca
     7861 ggtgccgttg tcatcagcac aaccgggcga tgtgctgctg tgctgttttg gttcatcggt
     7921 gccgaatcat gccgccattt actgcggcga tggtgagctg ctgcaccata ttcctgaaca
     7981 actgagtaaa cgagagaggt ataccgacaa atggcagcga cgcacacact ccctctggcg
     8041 tcaccgggaa tggcacgcat ctgcctttac ggggatttgc aacgatttgg ccgccgcatc
     8101 gaccttcgtg tgaaaacggg ggctgaagcc atccgcgcac tggccacaca gctcccggcg
     8161 tttcgtcaga aactgagtga cggctggtat caggtgcgca ttgccgggcg tgatgcaggt
     8221 gaaaccgaat tgtctgcccg tcttaatgag ccgctggcaa atggtgcagt gatccacata
     8281 gtaccgcgtc tggtgggagc taaaagtggc ggtgtgtttc aggcggtgct gggggcagct
     8341 gttatggcgg ttgctatatg gatgccgggg taggaattat ggcgagtaat ctgctgtttt
     8401 ctctcggtgc cagtatgacg cttggcggtg ttgcacagat gctggcaccg aaagccagaa
     8461 ctccccgtac acagacaacg gataacggca aacagaacac ctgtttctcc tcactggata
     8521 acatggttgc ccagggcaat gttctgcctg ttctgtacgg tgaaatgcgc gtggggtcgc
     8581 gggtggtatc tcaggagatc agcacggcag acgaagggga tggtggtcag gttgtggtga
     8641 ttggtcgctg atgcaaaatg tttcatgtga aaccgcctgc gggcggtttt gtcgtttatg
     8701 gagcgtgagg aatgggtaaa ggcagcagta aggggcatac cccgcgcgaa gcgaaggaca
     8761 acctgaaatc cacgcagttg ctgagtgtga ttgatgccat cagcgaaggg ccggttgacg
     8821 gtccggtgga tggattaaaa agcgtgctgc tgaacggtac gccggtcctg gacagcgagg
     8881 ggaagacaaa cttttccggt gttacggtgg tgttccgcgc cggcgagcag gagcagacac
     8941 cgccggaggg gtttgaatct tccggctcag agactgtgct gggtacggaa gtgaaatacg
     9001 acacgccgat cacccggacc atcacgtcgg caaacattga tcgtctgcgc ctgaccttcg
     9061 gtgtgcaggc actggtggaa accacctcaa agggggaccg gaatccgtcg gaagtccgcc
     9121 tgctggttca gatacagcgt aacggtggct gggtgacgga aaaagacatc accattaagg
     9181 gcaaaaccac ttcgcagtat ctggcctcgg tggtggtggg taacctgccg ccgcgcccgt
     9241 ttaatatccg gatgcgtagg atgacaccgg acagcaccac agaccagctg cagaacaaaa
     9301 cgctctggtc gtcgtacacc gaaatcatcg atgtgaaaca gtgctacccg aacacggcgc
     9361 tggtcggcgt gcaggtggat tcagagcagt tcggtaacca gcaggtgagt cgcaattatc
     9421 atcttcgcgg gcgcattctg caggtgccgt cgaactataa cccgcagacg cggcaataca
     9481 gcggtatctg ggacggaacg cttaagccag catacagcaa caacatggcc tggtgtctgt
     9541 gggacatgct cactcatccg cgctaaggca tggggaaacg tcgtggtgcg gcagatgtgg
     9601 acaaatgggc gctgtatgtc atcggccagt actgcgacca gtcagtgccg gacggatttg
     9661 gcggcacgga gccgcgcatc acctgtaacg cttacctgac cacacagcgt aaggcgtggg
     9721 atgttctcag tgatttctgc tcggcgatgc gctgtatgcc ggtatggaac gggcagacgc
     9781 tgacgttcgt gcaggaccga ccgtcggata aagtgtggac ctataaccgc agtaatgtgg
     9841 tgatgccgga tgatggcgcg ccgttccgct acagcttcag cgccctgaag gaccgccata
     9901 atgtcgttga ggtgaactgg attgatccgg acaacggtca tgagacggcg acagagcttg
     9961 tggaggacac gcaggccatt gtccgttacg gtcgtaacgt cacgaagatg gatgcctttg
    10021 gctgtaccag ccggggacag gcgcaccgcg ccgggctgtg gctgattaaa acggaactgc
    10081 tggaaacgca gaccgtggac ttcagcgtcg gcgcagaagg gcttcgccat gtaccgggtg
    10141 atgtcattga aatctgcgat gatgattatg ccggtatcag caccggtggt cgcgtactgg
    10201 cggtgaacag ccagacccgg acgctgacgc tcgaccgtga aatcacgctg ccgtcctccg
    10261 gtaccacgct gataagcctg gttgacggaa atggcaatcc ggtcagcgtg gaggttcagt
    10321 ccgtcaccga cggcgtgaag gtgaaagtga gccgggttcc tgacggcgtt gcagaataca
    10381 gcgtgtgggg gctgaagctg ccgacgctgc gccagcgcct gttccgctgc gtgagtatcc
    10441 gtgagaacga cgacggcacg tatgccatca ccgccgtgca gcatgtgccg gaaaaagagg
    10501 ccatcgtgga taacggggca cactttgacg gtgaccggcg cggcacggtg aatggtgtca
    10561 cgccgccagc ggtgcagcat ctgaccgccg aagtcaccgc agacagcggg gaatatcagg
    10621 tgctggagcg atgggacacg ccgaaggtgg tgaagggtgt gagcttcctg cttcgcctga
    10681 ccgtggcagc ggacgacggc agtgagcggc tggtcagcac agccaggacg acggaaacca
    10741 cataccgctt caggcagctg gcgctgggga attacagtct gacagtccgg gcggtaaatg
    10801 cccgggggca gcagggcgat ccggcgtcgg tatcgttccg gattgcggca ccggcagcgc
    10861 ctgtcactat tgaactgata ccggggtatt ttcagataac ggtggtcccg aaactggctg
    10921 tatatgaccc gacggtgcag tttgagttct ggttttcgga aaagcggatt gcggatatca
    10981 ggcaggttga aaccagcgcc cgctatcttg gcacggcgct gtactggata gccgccagta
    11041 tcaatatcag gccgggccat gattattatt tttacgttcg cagtgtgaac accgttggca
    11101 aatcggcatt tgtggaggcc gtcggtcggg cgagcgatta tgcggaaggt tatctgagtt
    11161 tttataaagg gttgatcaat aaaacgcatc tcggcaagga gttgtggacg cagattgata
    11221 acggtcagct tgcgccggac ctgactgaaa tcaggacgtc cattacgaat gtcagcaatg
    11281 aaatcacgca aaccgtcaat aaaacactgg aaaatcagag tgctgcaatc cagcagatac
    11341 agaaagttca ggttgataca aataataacc tgaacagcat gtgggctgtg aagctgcaac
    11401 agatgaagga cggacgcctt tatattgcgg gtatcggtgc cggtattgag aatacgccag
    11461 caggaatgca gagtcaggtg ctgctggcgg cagacaggat tgcgatgatt aatcctgcga
    11521 atggcaacac aaagccgatg tttgttggtc agagcgatca gatattcatg aacgacgtgt
    11581 tcctgaaacg cctgacggct ccgaccatta ccagcggcgg taatcctccg gcattttccc
    11641 tgacacctgg cggacggctg acggcgaaaa atgccgatat cagcggtaac gtgaacgcga
    11701 actccgggac gctcaacaac gtcacgatta ataagaactg tcgggttctg ggaaaattgt
    11761 ccgcgaacca gattgaaggc gatctcgtta aaacagtggg caaacctttc ccacgggact
    11821 cccgggcacc ggagaggtgg ccatcaggga ccattaccgt cagggtttat gacgatcagc
    11881 cgtttgatcg gcaaattgtt attcccgcgg tggcgtttcg cggtgctaaa catgagcgga
    11941 agaataacaa tatttattcg tcatgccgcc tgatagtgaa gaaaaacggt gctgaaattt
    12001 ataaccgaac gaccctggat aatacgctga tatatacggg tgttattgat atgcctgccg
    12061 gtcacggtca catgacgctg gagttttctg tatcggcatg gctggtaaat ggctggtatc
    12121 ccacagcaag tatcagcgat ttgctggttg ttgtgatgaa gaaagccact gcaggcatca
    12181 cgattagctg aattttataa cccagatacg ggcaccagaa atggtgcctt ttttattgca
    12241 gaaaagcgag aggtaattat gcgtaaagtt tgtgcagcca ttttgtccgc agccatctgt
    12301 ctggctgtat ccggtgcgcc tgcatgggcg tctgaacatc agtccacgct gagcgccggg
    12361 tatcttcagc cccatactga tatgccaggc agcgatgacc tgaagggcat taacgtgaaa
    12421 taccgttatg agtttacgga cacgctgggg ctggtgacgt cattcagcta tgccaacgct
    12481 gaagatgagc aaaaaacgca ttacagcgat a
//
LOCUS       DAETPZ010000106        12373 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.106, whole genome shotgun sequence.
ACCESSION   DAETPZ010000106 DAETPZ010000000
VERSION     DAETPZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.106"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(73..1309)
                     /locus_tag="K0W59_003424"
                     /pseudo
     CDS             complement(73..1309)
                     /locus_tag="K0W59_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            complement(1508..1592)
                     /locus_tag="K0W59_003425"
     tRNA            complement(1508..1592)
                     /locus_tag="K0W59_003425"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1556..1558),aa:Leu,seq:caa)
     gene            1788..2807
                     /gene="ahr"
                     /locus_tag="K0W59_003426"
     CDS             1788..2807
                     /gene="ahr"
                     /locus_tag="K0W59_003426"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HBI3024324.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2936..4450
                     /locus_tag="K0W59_003427"
     CDS             2936..4450
                     /locus_tag="K0W59_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HBI3024325.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGEKKISQT"
     gene            complement(4558..5640)
                     /gene="lptG"
                     /locus_tag="K0W59_003428"
     CDS             complement(4558..5640)
                     /gene="lptG"
                     /locus_tag="K0W59_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBI3024326.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKDDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5640..6740)
                     /gene="lptF"
                     /locus_tag="K0W59_003429"
     CDS             complement(5640..6740)
                     /gene="lptF"
                     /locus_tag="K0W59_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBI3024327.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            7007..8518
                     /gene="pepA"
                     /locus_tag="K0W59_003430"
     CDS             7007..8518
                     /gene="pepA"
                     /locus_tag="K0W59_003430"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBI3024328.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8652..9095
                     /gene="holC"
                     /locus_tag="K0W59_003431"
     CDS             8652..9095
                     /gene="holC"
                     /locus_tag="K0W59_003431"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HBI3024329.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9095..11950
                     /gene="valS"
                     /locus_tag="K0W59_003432"
     CDS             9095..11950
                     /gene="valS"
                     /locus_tag="K0W59_003432"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBI3024330.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(12004..>12373)
                     /locus_tag="K0W59_003433"
     CDS             complement(12004..>12373)
                     /locus_tag="K0W59_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HBI3024331.1"
                     /translation="RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRS
                     TLTYHGMLYRMCALVVISEITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQ
                     PDKGFLASISRGVMPSLPFL"
BASE COUNT         3046 a         3238 c         3318 g         2771 t
ORIGIN      
        1 tttctgctca ttattagctc cttctgatgc cattctattt caggaaggag tgtccgttaa
       61 actcaggcta cctcattctg aggtgagcat atcacctctg ttcaggtggc caaattcagt
      121 gtgccacttc aaacatcaac ctacgctccc caagatgttc cgccttatgg atgtaagctg
      181 ctcgcacaga gctacgctcc tggtgactca tctgccgctc taccgcatcc ctcgaccata
      241 atcccgactc aatcaacgaa ctacaagcca tcgtcctgaa gccatgccca caaacctcga
      301 ccttcgtgtc ataccccatt acgcgcaaag ccttgttcac cgtattctca ctcatcggct
      361 tacgtggatc gtgatcgccc acaaaaatca gctttcgatt tccgctcatg cttttgatct
      421 tttccaaaat acttagggcc tgccgcgaca aaggtacaag gtgtggtgtc cgcatcttcg
      481 agccacgctg agaatgcttg actccttcca gttgctcacg ctcacctggg attgtccaca
      541 tagccgtttc aaaatctact tctgaccagc gggcaaaacg cagctcgctc gaacggatga
      601 aaaccaataa agtgagttca accgccagtc gagttaacgg tctgccagaa tagtgatcta
      661 tgcgatgaag taattcaggt atacggttca gttcaagagc tgcacggtgc tgtcttttcg
      721 ccgtagcaac cgcacctgca atctcttgcg cggggttgta gtcgattaag ccgctctgaa
      781 cggcaaagcg cataatcgcg gtagtacgct gctgtaaacg ggcggcaact tcgagccgcc
      841 cggatgactc gactgcttta atgggtacaa gaagatcccg cgtcttcagt tccgcaatgt
      901 tccgcttacc gatagcagta aagagattat cctccagact tttcaaaaca cgagcgctat
      961 gcgatgccga ccacttctga ttgctggcgt gccagtctct ggcgactact tcaaacgtta
     1021 tcgcctcttg ttcctgttct accttaacag ctttcttgtt ttcactggga tcgacaccat
     1081 tagctaaaag cttacgagcc tcatcccggc gtgccctggc atccgccaac gacacttcgg
     1141 ggtatttccc caatgcaagc atcttctctt tgccgccaaa gcgataacgt agccgccagt
     1201 atttggagcc gttagggtga accagcaaaa ccatgccttc accgtcggtc agcttatagg
     1261 cttttgcttc aggcttagcc gaacgaacct tcacatcact cagagccatg atgagtatcc
     1321 tttcaagggt tctgtgtggg tacaaacatt atcgaaccgg gaaatacccg cagttgtacc
     1381 cgcatcagta agttgatgta gattgaatca ggttgactta ggttgagtga aaaagcgagg
     1441 aaagccttgc ggatactgga ttttaggcac aaaaaaagac gtccgttgac gtctattgat
     1501 gttccgatgg tgccgaaggc cggactcgaa ccggcacgta tttctacggt tgattttgaa
     1561 tcaactgcgt ctaccgattt cgccacttcg gcactgaaga ggtatgcgga aaacgttgtg
     1621 gattatacct gtcgcacgcc accatgcaag cgccagcttg cacccacctc gctaagtgct
     1681 gaaattttca gcattacact cagcgatatg taacctttgt cacactccag gccccccgcc
     1741 ctgccatgct ctacacttcc caaacaacac cagagaagga cccaaaaatg tcgatgataa
     1801 aaagctatgc cgcaaaagaa gcgggcggcg aactggaagt ttatgagtac gatcccggtg
     1861 agctgaagcc acaagatgtt gaagtgcagg tggattactg cgggatttgt cattccgatc
     1921 tgtcgatgat cgataacgaa tggggatttt cacaatatcc gctggttgcc gggcatgagg
     1981 tgattgggcg cgtggtggca ctcgggagcg ccgcgcagga taaaggtttg caggtcggtc
     2041 agcgtgtcgg gattggctgg acggcgcgta gctgtggtca ctgcgacgcc tgtattagcg
     2101 gtaatcagat caactgcgag caaggtgcgg tgccgacaat tatgaatcgc ggtggctttg
     2161 ccgagaagtt gcgtgcggac tggcaatggg tgattccact gccagaaaat attgatatcg
     2221 agtccgccgg gccgctgttg tgcggcggta tcacggtctt taaaccactg ttgatgcacc
     2281 atatcactgc taccagccgc gttggggtaa ttggtattgg cgggctgggg catatcgcta
     2341 taaaacttct gcacgcaatg ggatgcgagg tgacggcctt tagttctaat ccggcgaaag
     2401 agcaggaagt actggcgatg ggtgccgata aagtggtgaa tagccgcgat ccgcaggcac
     2461 tgaaagccct gtcggggcag tttgatctca ttatcaatac tgtgaacgtc agcctcgact
     2521 ggcagcctta ttttgaggcg ctgacctatg gcggtaattt ccatacggtc ggtgcggttc
     2581 tcacgccgct gtctgttccg gcctttacgt taattgcggg cgaccgcagc atctctggtt
     2641 ctgctaccgg cacgccttat gagctgcgaa agctgatgcg ctttgccgcc cgcagcaagg
     2701 ttgcgccgac aaccgaactg ttcccgatgt cgaaaattaa cgacgccatt cagcatgtac
     2761 gcgatggtaa agcgcgctac cgagtagtcc tgaaagccga cttctgatcc ctttgccggg
     2821 tgctgtcgcc cggcacttaa atctctttgg aaggcgtctc gcaatctgct gaatttagcg
     2881 gtgaagagtg cacagccgtt gcctatactc caggcaggaa actggaggaa atctcatgag
     2941 cgaacccctg ttaattgccc gcacgccgga cacagaactg tttttactgc cgggaatggc
     3001 taaccgtcac gggctgatta ctggcgcaac ggggacgggt aaaaccgtta cgctgcaaaa
     3061 actggcagag tcattgtcgg aaatcggcgt gccggtgttt atggctgatg tgaaaggcga
     3121 tctgaccggt atcgcgcagg caggaacggc gtcggaaaaa ctgctcacaa ggcttaaaaa
     3181 tatcggcgtc aatgactggc aaccgcatgc caatccggtg gtggtgtggg atatctttgg
     3241 cgagaaaggc cacacggtgc gggcgacggt ttcggatctg gggccgctgt tgctggcgcg
     3301 actgttgaat ctcaacgatg tgcaatctgg cgtgctgaat attatcttcc gcattgcgga
     3361 cgatcaggga ttgttgctgc tcgactttaa agatttgcgg gcgattaccc agtacatcgg
     3421 cgataacgcc aaatccttcc agaatcagta cggaaatatc agtagcgcat cggttggtgc
     3481 catccagcgc ggattactgt cgctggaaca gcaaggcgca gcacacttct ttggtgagcc
     3541 gatgctggat atcaaagact ggatgcgcac cgatgccaac ggtaaaggcg ttatcaatat
     3601 cctcagcgcc gagaaacttt atcagatgcc gaaactgtac gccgccagcc tgctgtggat
     3661 gctttcagag ttgtatgaac aattgccgga agcgggcgat ctggagaaac caaaactggt
     3721 gtttttcttc gacgaggcac atctgctgtt taacgatgca ccgcaggtac tgctggataa
     3781 gattgagcag gtgataaggc ttattcgctc aaaaggcgtg ggcgtctggt tcgtttcgca
     3841 aaacccgtct gatattccgg ataatgtgct cggccagctc ggtaatcgtg ttcagcacgc
     3901 tttgcgggct tttacgccaa aagatcagaa agcagtgaag acagcggcgc aaaccatgcg
     3961 ggccaatccg gcatttgata ccgaaaaggc aatccaggaa ctggggaccg gcgaggcgtt
     4021 aatctcgttt ctcgatgcaa aaggaagtcc ttctgtggtg gaacgggcga tggtgatcgc
     4081 accttgttcg cgaatggggc cggtgacgga agatgagcgt aatggcctga ttaatcactc
     4141 cccggtgtat ggcaaatatg aagatgaggt ggaccgcgag tccgcctatg agatgctgca
     4201 aaaaggcttt caggccagta ccgagcagca aaataatccc cccgtgaaag ggaaagaggt
     4261 ggcggtggat gacggtattc ttggtggatt gaaggatatt ttgtttggca ctaccggacc
     4321 acgcggcggg aagaaagatg gtgtggtgca aacaatggcg aaaagcgccg ctcgccaggt
     4381 gacgaatcag attgtacgcg ggatgttggg gagtttgctg gggggggaga aaaagataag
     4441 tcagacgtga tctgtgcccc tcaccctaac cctctcccca aaggggcgag gggaccgttc
     4501 gaactcgtat ttgactttgt caacaataat aaagggagct ttcgctccct tgttagatta
     4561 cgattttctc attaacagcc acaggctgat taagaagaag ctggcacttg gcaacagtgc
     4621 gccgatgatc ggcgggatgc cgtaaaccaa cgtcagcggg ccgaagatct ggtccagtac
     4681 gtagaagaca aaaccgaagc tgatacctgt gaccacacgc acgcccatcg gtacgctacg
     4741 cagtgggcca aagataaacg acagcgccat cagcatcatc accgccacgg atagcggctg
     4801 gaagattttg ctccacatgt tgagctgata acgtccggca tcctgaccgc tcgacttcag
     4861 atacttcacg tagttgtgca aaccactgat ggagagtgca tccgggtcca gcgccaccac
     4921 gcccagtttg tctggcgtga ggtcggtttt ccaggtgccg ctcaccgtct gcgaaccggt
     4981 aatctgtttc ggattggtca gatcagattc atcaacctgc gacagacgcc agactttatg
     5041 ttccgggtca aacttcgcgg tagcggcata acgtacggat tgcagacgac gattctcgtt
     5101 aaaggcataa atactgatgc cacctaacac ctcgtcatct ttaacccgct caatgtagac
     5161 gaagttgttg ccatctttcg cccataaacc ttgctgggta gagagcaacg agccgccgta
     5221 catcgcctgc gcacggtagt tacgcgccat ctgttcgccc tgcggcgcga cccattcgcc
     5281 aatcgccatc gtcagcaaga ccagtggaat ggcggttttc atcaccgaca gcgctacctg
     5341 catacgggta aaaccagaag cctgcatcac caccagttcg ctgcgctgcg ccagcatccc
     5401 aagaccaagc aacgccccaa gaagagccgc catcgggaag aagatctgca catctttcgg
     5461 cacgctcagc aaggtataca ttcctgcgcc taacgcgtcg taactcccct gcccggcttt
     5521 tttcagctga tcgacaaatt tgataatgcc cgacagcgac accagcatga acagcgtcat
     5581 catgatggtg gtgaaaatag ttttaccgat atagcggtca agtacgccaa aaggttgcat
     5641 cacaccgctc ctttacgcga aaaactggcg cgcaggcggc ggaccggcac ggtgtcccaa
     5701 aggttgagaa caatcgccaa agccagataa atcaggttaa cggtccacat ccacagtgtc
     5761 gggtccagct tacctttacc gccgttcgat ttcagggagg tctggatcag gaagaaaagt
     5821 agatacagca gcatggctgg cagcatcgac agtacgcgtc cctgacgcgg gttaaccacg
     5881 ctcaacggta caaccataag tgccatcata aacacggtga ataccaacgt gatacgccag
     5941 ttcagttctg cgcgagcacg atcggtgtca gtgttccaca atgtgcgcat gtccatctgg
     6001 tcggtatcgt tcgggtcgag cgccaccgcc tggtgaccaa tgatcgcctg ataatcctgg
     6061 aaatccgtaa tgcggaaatc acgtaacagt gcagtgcctt cgaagcgcgt tccctggttg
     6121 agagtgacga cctgggagcc gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc
     6181 acagaaggac gtgcattacc ttttggtcga atttgtgcga ggaacacatc tttgaaatcg
     6241 ctgccgtcaa cgctttcgat gaacagcacc gagctgccat tagtcgcttg ctggaattgc
     6301 ccttgcgcca gcgccgccat gccagggttc gctttcgctt ctgctaacac ttcatcctga
     6361 tgacgcgatg accacggtcc cgcccacatc acgttaaccg ccgcgacgat tgccgtgaat
     6421 accgcaagga tcattgccgc tttcaccaga accgctttgc tcaagccgca ggcatgcatt
     6481 accgtaattt cactttcggt atacagtttg cccagcgtca tcagcagccc gaggaacagg
     6541 cttaatggca ggataagctg cgccatttcc ggcacgccca acccgagaag ggagagcacc
     6601 agattcgccg gaatatcgcc gtcaaccgct gcgccgagga tcctcactaa cttttgacag
     6661 aagaagatca aaagcaagat gaagagtatc gccagctggc ttttgagcgt ctcccgcacc
     6721 agatatctta tgattatcac tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt
     6781 agcttgtttc atggcttaaa cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga
     6841 taattatact caacggattc acctctcaga ttttgttctg acgtgccaat gccgtaataa
     6901 cgttaagatt aacacgaagt catcgcaaca gcggacatga gttacgaaag cttgcaattc
     6961 tatctgtagc caccgccgtt gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt
     7021 aaaaagcggt agcccggaga aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc
     7081 acgtcgcctt tctccgattg cagaacagct cgataaaatc agcgatgggt acatcagcgc
     7141 cctgctacgc cggggcgaac tggaaggaaa accggggcag acattgttgc tgcaccatgt
     7201 tccgaatgta ctttccgagc gaattctcct tattggttgc ggcaaagaac gtgagctgga
     7261 tgagcgtcag tacaagcagg ttattcagaa aaccattaat acgctgaatg atactggctc
     7321 gatggaagcg gtctgctttc tgactgaact gcacgttaaa ggccgtaaca actactggaa
     7381 agtgcgtcag gctgtcgaga cagcaaaaga gacgctctac agtttcgatc agttgaaaac
     7441 gaataagagc gaaccgcgtc gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg
     7501 tgaactgacc agcggtgagc gcgcgatcca gcacggtctg gcgattgccg ccgggataaa
     7561 agcagcgaaa gatctcggta atatgccgcc gaatatctgt aacgccgctt atctcgcctc
     7621 tcaggcgcgc cagctggctg acagctacag caagaatgtc atcacccgcg ttatcggcga
     7681 acagcagatg aaagagctgg gaatgcactc ttatctggcg gtcggtcagg gttcacaaaa
     7741 cgaatcgctg atgtcggtga ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat
     7801 cgtgctggtg ggtaaaggtt taaccttcga ctccggcggt atctccatca agccttcaga
     7861 aggcatggat gagatgaagt acgatatgtg cggcgcggcg gcggtttacg gcgtgatgcg
     7921 tatggtcgcg gagctgcaac tgccgattaa cgttattggc gtgctggcag gctgtgaaaa
     7981 catgcctggc gggcgtgcct atcgtccggg cgatgtgtta accaccatgt ccggtcaaac
     8041 cgttgaagtg ctgaacaccg atgccgaagg ccgcctggta ctgtgcgacg tgttaactta
     8101 cgttgaacgt tttgagccgg aagcagtgat tgacgtggcg acgctgaccg gtgcctgcgt
     8161 gatcgcgctg ggtcatcata ttactggtct gatggcgaac cataatccgc tggcccatga
     8221 actgattgcc gcgtctgaac aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga
     8281 gtatcaggaa cagctggagt ccaattttgc cgatatggcg aacattggcg gtcgtcctgg
     8341 tggggcgatt accgcaggtt gcttcctgtc acgctttacc cgtaagtata actgggcgca
     8401 cctggatatt gcaggaaccg cctggcgttc tggtaaagca aaaggcgcaa ccggtcgtcc
     8461 ggtagcgttg ctggcacagt tcctgttaaa ccgcgctggg tttaacggcg aagagtaagt
     8521 ttgcgtttta ccctcaccct aaccctctcc ctaagggaga gggaaccgat cgagcgcaat
     8581 agaaattcat tgctcatctt tcagggtgag gaatgcagca atatttaaat ccaccacaag
     8641 aagccccata tatgaaaaac gcaacgttct accttctgga caatgacacc accgtcgatg
     8701 gcttaagcgc cgttgagcaa ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta
     8761 agcgcgtact catcgcctgt gaagatgaaa agcaggctta ccggctggat gaagccctgt
     8821 gggcgcgtcc ggcagaaagc tttgttccgc ataatttagc gggagaagga ccgcgcggcg
     8881 gtgcgccggt ggagatcgcc tggccgcaaa aacgcagcag cagcccgcgc gatatattga
     8941 ttagtctgcg aacaagcttt gcagattttg ccaccgcttt cacagaagtg gtagacttcg
     9001 ttccttatga agattctctg aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg
     9061 ctggtttcaa cctgaatacg gcaacctgga aataatggaa aagacataca acccacaaga
     9121 tatcgaacag ccgctttacg agcactggga aaagcagggc tactttaagc ctaatggcga
     9181 tgaaagccag gaaagtttct gcatcatgat cccgccgccg aacgtcaccg gcagtttgca
     9241 tatgggtcac gccttccagc aaaccatcat ggataccatg atccgctatc agcgcatgca
     9301 gggcaaaaac accctgtggc aggtcggtac tgaccacgcc gggatcgcta cccagatggt
     9361 cgttgagcgc aagattgccg cagaagaagg taaaacccgt cacgactacg gccgcgaagc
     9421 tttcatcgac aaaatctggg aatggaaagc ggaatctggc ggcaccatta cccgtcagat
     9481 gcgccgtctc ggcaactccg tcgactggga gcgtgaacgc ttcaccatgg acgaaggcct
     9541 gtccaatgcg gtgaaagaag ttttcgttcg tctgtataaa gaagacctga tttaccgtgg
     9601 caagcgcctg gtaaactggg acccgaaact gcgtaccgct atttctgacc tggaagtgga
     9661 aaaccgcgaa tcgaaaggtt cgatgtggca catccgctat ccgctggctg acggcgcgaa
     9721 aaccgcagac ggtaaagatt atctggtggt tgcgaccact cgcccggaaa ccctgctggg
     9781 cgataccggt gtagctgtta acccggaaga tccgcgttac aaagatctga ttggcaaata
     9841 tgtcattctg ccgctggtta accgtcgtat tccgatcgtt ggcgacgaac acgccgacat
     9901 ggaaaaaggc accggctgcg tgaagatcac cccggcgcac gactttaacg actatgaagt
     9961 cggtaaacgt cacgccctgc cgatgatcaa catcctgacc tttgacggtg atatccgtga
    10021 aagcgcccag gtgttcgata ccaaaggtaa cgaatctgac gtttattcca gcgagatccc
    10081 ggcagagttc cagaaactcg agcgttttgc tgcacgtaaa gcagtcgttg ctgcggttga
    10141 tgcactcggc ctgctggaag aaattaaacc gcacgacctg accgttcctt acggcgaccg
    10201 tggcggcgtg gttatcgaac cgatgctgac cgaccagtgg tacgtgcgtg ccgatgtact
    10261 ggcgaaaccg gcagttgaag cggttgagaa cggcgacatt cagttcgtac cgaagcagta
    10321 cgaaaacatg tacttctcct ggatgcgcga tattcaggac tggtgtatct ctcgtcagct
    10381 gtggtggggt caccgtatcc cggcatggta tgacgaagcg ggtaatgttt atgttggccg
    10441 caacgaagaa gaagtgcgca aggaaaataa cctcggtgcc aacgttgccc tgcgtcagga
    10501 cgaagacgtt ctcgacacct ggttctcttc tgcactgtgg accttctcta cccttggctg
    10561 gccggaaaat accgacgccc tgcgtcagtt ccacccaacc agcgtgatgg tatctggttt
    10621 cgacatcatc ttcttctgga ttgcccgcat gatcatgatg accatgcact tcatcaaaga
    10681 tgaaaatggc aaaccgcagg tgccgttcca cactgtttac atgaccggtc tgattcgtga
    10741 cgacgaaggt cagaagatgt ccaaatccaa gggtaacgtt atcgacccgc tggatatggt
    10801 tgacggtatt tcgctgccag aactgctgga aaaacgcacc ggcaatatga tgcagccgca
    10861 gctggcggac aaaatccgta agcgcaccga gaaacagttc ccgaacggca ttgagccgca
    10921 cggcaccgat gccctgcgct tcaccctggc ggcgctggcg tctaccggtc gtgacatcaa
    10981 ctgggatatg aagcgtctgg aaggctaccg taacttctgt aacaagctgt ggaacgccag
    11041 ccgctttgtg ctgatgaaca cagaaggtca ggattgcggc ttcaacggcg gcgaaatgac
    11101 gctatcgctg gcggaccgct ggattctggc ggagttcaac cagactatca aagcgtaccg
    11161 cgaagcgctg gacagcttcc gcttcgatat cgccgcaggc attttgtatg agttcacctg
    11221 gaaccagttc tgcgactggt atctggagct gaccaagccg gtaatgaacg gtggaacaga
    11281 agccgaactg cgcggtactc gccatacgct ggtgactgta ctggaaggtc tgctgcgcct
    11341 cgcgcatccg atcattccgt tcatcaccga aaccatctgg cagcgtgtga aagtactttg
    11401 cggtatcact gccgacacca tcatgctgca gccgttcccg cagtatgatg catctcaggt
    11461 tgatgaagcc gcactggccg acaccgagtg gctgaaacag gcgatcgttg ctgtacgtaa
    11521 catccgtgca gaaatgaaca tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg
    11581 cagcgcggat gcagaacgtc gcgtaaatga aaaccgtggc ttcctgcaaa cgctggcgcg
    11641 tctggaaagc atcaccgtgc tgcctgccga tgacaaaggt ccggtttccg ttaccaagat
    11701 catcgacggc gcagagctgc tgatcccgat ggctggcctc atcaacaaag aagatgagct
    11761 ggcgcgcctg gcgaaagaag tggcgaagat cgaaggtgaa atcagccgca tcgagaacaa
    11821 gctggcaaat gaaggctttg tcgcccgcgc accggaagcg gttatcgcga aagagcgtga
    11881 gaagctggaa ggttatgcag aagcgaaagc caagctgatt gaacagcagg ctgttatcgc
    11941 cgcactgtaa tcaacacagt gatgaaaacg aaggccggag catgctccgg ccttttttat
    12001 ctcttacaga aatggtaaag aaggcataac tccccgagaa atactggcta agaagccttt
    12061 atctggttgt tcttctttat tgattaaagg aaggtcatcc aaatcgccca gcaaatacgt
    12121 attttttgcc tgccagtcaa tcatccatat tttcagcagt gggtaagcca gaccacccgt
    12181 aatctcggat atcaccacca acgcacacat gcgataaagc ataccgtggt acgttaaagt
    12241 tgagcgaaaa cgaatacgcc catcattcag tgacaggttg ctcataaaat gatttcgcaa
    12301 cgacaccgtt aaataactgg tgctaacagc aatcccaaaa taataaatta actgcgcgat
    12361 tatcatttta cgt
//
LOCUS       DAETPZ010000107        12315 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.107, whole genome shotgun sequence.
ACCESSION   DAETPZ010000107 DAETPZ010000000
VERSION     DAETPZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.107"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            59..1330
                     /locus_tag="K0W59_003434"
                     /pseudo
     CDS             59..1330
                     /locus_tag="K0W59_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804911.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
     gene            1343..2113
                     /locus_tag="K0W59_003435"
     CDS             1343..2113
                     /locus_tag="K0W59_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBI3024332.1"
                     /translation="MKISGLAFNTRHVNCVEGNTTLYALPKPEVVLRWREQTTDDFRF
                     CFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALWHFL
                     DSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHASRPHSEAIR
                     DAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLH
                     TPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            2166..2561
                     /locus_tag="K0W59_003436"
     CDS             2166..2561
                     /locus_tag="K0W59_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HBI3024333.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2602..3345
                     /gene="cmoA"
                     /locus_tag="K0W59_003437"
     CDS             2602..3345
                     /gene="cmoA"
                     /locus_tag="K0W59_003437"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HBI3024334.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     NAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGSAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            3342..4313
                     /gene="cmoB"
                     /locus_tag="K0W59_003438"
     CDS             3342..4313
                     /gene="cmoB"
                     /locus_tag="K0W59_003438"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HBI3024335.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4478..6907)
                     /gene="torZ"
                     /locus_tag="K0W59_003439"
     CDS             complement(4478..6907)
                     /gene="torZ"
                     /locus_tag="K0W59_003439"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HBI3024336.1"
                     /translation="MALTIREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAANRTILMAGWGIQRQQYGEQKHWMLVTLAVMLGQIGTPGGGFGFSYHYS
                     NGGNPMRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(6932..8032)
                     /locus_tag="K0W59_003440"
     CDS             complement(6932..8032)
                     /locus_tag="K0W59_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HBI3024337.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHSKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(8420..9166)
                     /gene="cutC"
                     /locus_tag="K0W59_003441"
     CDS             complement(8420..9166)
                     /gene="cutC"
                     /locus_tag="K0W59_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBI3024338.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGGLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(9180..9746)
                     /locus_tag="K0W59_003442"
     CDS             complement(9180..9746)
                     /locus_tag="K0W59_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBI3024339.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            9962..11695
                     /gene="argS"
                     /locus_tag="K0W59_003443"
     CDS             9962..11695
                     /gene="argS"
                     /locus_tag="K0W59_003443"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBI3024340.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRSLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            11872..12162
                     /locus_tag="K0W59_003444"
                     /pseudo
     CDS             11872..12162
                     /locus_tag="K0W59_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
     gene            complement(12178..>12315)
                     /locus_tag="K0W59_003445"
     CDS             complement(12178..>12315)
                     /locus_tag="K0W59_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3024341.1"
                     /translation="QRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHY
                     Q"
BASE COUNT         3071 a         3106 c         2972 g         3166 t
ORIGIN      
        1 tgatttaata tcatgaaata tgtaagctcg tatcgctctt gggcgtctgg aggtatcgat
       61 ggataaagtc aaatatccaa caggcgtcga aaaccacggc ggcacattac gcatctggtt
      121 taattttaaa ggtaaacgtg tcagggaaaa tcttggtgtc cctgacactg ccaagaacag
      181 gaagatcgcc ggggaactgc ggacatcggt atgttttgcc atccgcacag gaagctttga
      241 ttatgctgca cagttccctg actcccctaa ccttcaggct tttggggtaa gtaaaaaaga
      301 aattacggtg aaggaacttg aagaaaagtg gctggatctg aaacgaatgg aaatctctgc
      361 aaatgcattc aatcgctatg aatccgttgc aagaacgatg gttccgaaaa ttggaggcgg
      421 cagactggtg tcatcggtaa ccaaagagga attgctgtat atcaggaaag atttgctgac
      481 cgggtatcag aattcaacga aaaacaaagc agcagcaaaa ggacggagcg tcgttactgt
      541 aaattattac atgacgacaa tcgctggaat gtttcagttt gctgcagatc acggttactt
      601 agaagcaaat cccttccagg gaattaagcc tcttaaaaga gccagggcag agccagatcc
      661 gttaactcgt gacgaattta ttcgcctgat agatgcttgc cgacatcagc agacgaaaaa
      721 cctgtggtca ttggctgtgt acacaggaat gcgtcacggt gaactggtct ccctggcctg
      781 ggaagatatc gatatgaaag caggaactat tactatcagg cgcaattata cgaaactcgg
      841 tgagttcact ctacctaaaa ctgaagcaag tacaaacagg gttgtgcatc ttatccagcc
      901 cgctatcagt gtcctgaaaa atcaggctga aatgacaaga ctgggtaagc agcaccacat
      961 caaggttcaa ctacgtgaat atggacgttc agtgaatcat gaatgtactt tcgtatttaa
     1021 cccccaggtg gttagaaaaa gcaaacaggt aggttttatc tacaaggtag attccattgg
     1081 cgactcatgg gaaacagcca ttaagcgtgc gggcatcagg caccggaaag cataccagtc
     1141 acgacacact tatgcgtgct ggtcattatc tgccggagca aacccaagct tcattgccag
     1201 ctagatgggc catgcaagtg cccagatggt gttcaatgta tacggagcat ggatgactga
     1261 cagtaatgca gaacagatcg caatgctgaa tcagaagctg gcagattatg tcccatggtc
     1321 accaaagtga cacaagagac ttattaaaat cagtgggtta gccttcaata cccgtcatgt
     1381 taactgcgtg gagggcaaca ccacgcttta cgccctgccg aaacccgagg ttgtcctgcg
     1441 ctggcgtgaa cagaccacag atgacttccg cttctgtttt aagtttccgg cgaccatttc
     1501 gcatcaggca gcattacgac attgcgatga tttagtgact gaatttttga cccgcatgtc
     1561 accgttggct ccgcgcattg ggcaatactg gctgcaactg cctgccacat tcggcccacg
     1621 ggagctgcct gcgctttggc attttctcga ttctctccct ggcgaattta attatggtgt
     1681 ggaagtccgc catccacagt ttttcgccaa aggggaagag gaacaaacgc ttaatcgcgg
     1741 tttacatcag cgcggcgtta atcgggtgat tttagacagc cgcccggttc atgcatcacg
     1801 tccacacagt gaagctattc gcgacgctca acgaaaaaaa cctaaagttc cggtacatgc
     1861 tgtactgacg gcgacaaatc cgctgatccg ttttatcggt agtgatgata tgacgcaaaa
     1921 ccgggaatta tttcaggtct ggttacaaaa attagcgcag tggcatcaga ccactacgcc
     1981 ttatcttttt ttacatacgc cagatattgc ccaggccccg gaactggtac ataccctgtg
     2041 ggaagactta cgtaaaacgc ttccagagat cggagcagtt ccggctattc cacagcaatc
     2101 ttctcttttc tgaatttgcc acctatcata gacaggtgcc atcggccatt ttaaagggag
     2161 tttgtatggt aagcgcgcta tatgccgttt taagtgcgtt gttattaatg aagttctctt
     2221 ttgatgtcgt tcgcctgcga atgcagtacc gcgttgccta tggtgacggt ggttttagcg
     2281 aactgcaaag cgctattcgc attcatggta acgcggtgga atatattccc atcgcgattg
     2341 tgctgatgct gtttatggaa atgaatggcg cagaaacctg gatggtgcat atttgcggca
     2401 tcgttttgct tgctggtcgt ctgatgcatt attacggttt tcatcaccgt ctgtttcgct
     2461 ggcgacgttc cggcatgagc gccacctggt gtgcgctgtt gctgatggtg ctggcgaatc
     2521 tttggtatat gccctgggag ttggttttct ccctgcgtta gcgcacaata cgccactttc
     2581 tttttcccgg atttttacgt tatgtctcac cgcgacacgc tattttctgc ccctatcgcc
     2641 agactgggcg actggacctt tgatgaacgg gtagctgaag tcttcccgga tatgatccag
     2701 cgttccgttc ccggctattc caatattatt tccatgattg gtatgttagc cgagcgcttc
     2761 gttcaacctg gtacgcaggt ttacgatctg ggttgttctc tgggcgcggc gacgctctcg
     2821 gtgcgtcgca acattcatca tgataattgc aaaattattg ccatcgacaa ctccccggcg
     2881 atgattgaac gctgccgtcg tcatattaac gcctataaag cccctacgcc agtagacgtc
     2941 attgaaggtg atattcgcga tatcgccatt gaaaacgcat cgatggtggt gctgaatttt
     3001 accctgcaat tcctggaacc ttccgagcgc caggcattac tggataaaat ttatcaagga
     3061 ctgaacccgg gcagcgcgct ggtgctttcg gaaaaattca gtttcgaaga tgccaaagtt
     3121 ggtgaactgt tgttcaacat gcaccacgac tttaaacgtg ccaacggtta cagcgaactg
     3181 gagatcagcc agaagcgcag catgctggaa aacgtgatgc tgaccgattc cgtggaaacc
     3241 cataaagcac gcctgcataa agccggtttt gagcatagcg agctgtggtt ccagtgcttt
     3301 aactttggtt cactggtggc attaaaagca gaggacgctg catgatcgac tttggtaact
     3361 tttattctct gattgccaaa aatcatcttt cacactggct cgaaacgctg cccgcgcaga
     3421 ttgctaactg gcagcgcgag cagcagcacg ggctgtttaa gcagtggtcc aacacggtgg
     3481 aatttctgcc tgaaattaaa ccgtatcgtc tggatttatt gcatagcgta accgccgaaa
     3541 gcgaagagcc actgagcacc gggcaaatta aacgcattga aacgcttatg cggaacctga
     3601 tgccatggcg caaagggccg ttctcactgt atggcgtcaa catcgatacc gaatggcgtt
     3661 ccgactggaa atgggatcgt gttctgcccc atctttctga tttaaccgga cgcaccattc
     3721 tggatgtcgg ctgcggcagc ggttatcaca tgtggcgcat gattggcgca ggcgcgcacc
     3781 tcgcggtggg gatcgaccct acgcaactgt ttctgtgcca gtttgaagcg gtgcgtaaac
     3841 tactgggtaa cgatcagcgc gcacatttgt taccgttagg tattgaacag cttccggcac
     3901 tgaaagcctt tgataccgtc ttttcgatgg gcgtgcttta ccatcgccgt tcgccgctgg
     3961 agcatctctg gcagttgaaa gatcaactgg tgaatgaggg tgaactggtg ctggaaacgc
     4021 tggttattga tggcgatgag aacacggtgc tggtaccagg cgatcgttac gcgcaaatgc
     4081 gtaatgtcta tttcatccct tccgcgctgg cgctgaaaaa ctggctgaag aagtgtggtt
     4141 ttgtagatat ccgcattgtg gatgtgtgcg ttaccactac tgaagagcag cgacgcactg
     4201 aatggatggt taccgaatct ctgtccgatt tcctcgaccc acatgatccg agtaagacag
     4261 ttgaaggtta tcctgcgcct aaacgcgcgg tgctgattgc gcgcaagccg taaaggtctg
     4321 gtattactgc cggatgcggc gtgaacgcct tatccggcct acaaaccctt gctaattcaa
     4381 tctattgcag ggactatgta ggcctgataa gcatagcgca tcaggcagtt ttacgtttgc
     4441 ataacctcag cgcccgtttc cgggcgctat tcacttctta cgcctgaaca gcaggcttat
     4501 caaatgccgt taacttcggt gcgttacccg tatatttttc gatatacacc agcgcagagt
     4561 taccggcaca ggcatttgcc agctgcgagc tggggatatc cgccgttaac acgttcgcac
     4621 tgccgttttt acacaagccg ttttccagat ctggccatgc accttcatgc acgcatacca
     4681 cgcctttttt gatcccgtca gtcaccaccg cgcctgtcaa aatctgacca cgtttgttcc
     4741 agacacgcac cagatcgcca ttcgcaatac caaatcgagc agcatcttcg gtgtgaatag
     4801 tgatcggttc acgatcggca accgcatatt ttttacgcag ttccgcatag ttaagctgac
     4861 tgtgtaaacg gtgtgccgga tgtgcggtca gaagctgcaa ctgcttctcg tcggcggtac
     4921 ccttccactc atcaggcgca agccaggttg ggtgtgccgg gcaatcctta tagccaaatt
     4981 tttccagcgt tttggagtaa atctcaattt tgccgcttgg cgtacccagc gcatttttca
     5041 ccggatcggc gcggaaatca ccataacgaa cgtactgttc gttcttctcg ctgcggcgca
     5101 tttcgatcag tttattttgc tgccagaagg cattaaacat tggcatagtg acgcgttgcg
     5161 cacgggcccc tttctgagcg gcatcataga aaaatttcag ccacgccatt tcatctttac
     5221 cttcggtata gatctctttt ccgccaggtt tgagtaattc cgcaagatcg gcaaacacgt
     5281 caaaatcgtt acgcgcttca aattgcggag caacagcctg cttcatcggc acaatatgct
     5341 ggttgctgta atcaccggtc atcgtcaagt cattgcgctc aaacgatgtg gtgatcggta
     5401 atacgatatc tgcgtgtttg gctgctgcgg tccagtagca ttcagaaacg acgatcatct
     5461 ccggtttctg ccaggctttg atcagacgat tagtatcctg gtgatgggta aagttaccgc
     5521 cacccgccca ccagatcatt ttgatattcg gatacgtttg ttctttaccg ttatgctgat
     5581 atttgccgcc aggattttcc agcgcatcga cgatacgggc aacaggaatc gccgtcattc
     5641 cgccatcatc agcggcctcg ctggcctgtc cggcaatcgc cgcagacatt tccggcagca
     5701 cgccgccaac acgcatcggg ttgccaccat tggagtaatg ataagagaaa ccaaaaccgc
     5761 cgcccggtgt accaatctgc cctaacatca ccgccagcgt caccagcatc cagtgtttct
     5821 gttcaccata ttgctggcgc tgaattcccc agcctgccat cagaattgtg cgattcgccg
     5881 ccatcagttc agcgagtttg actatctgcg cttccggtac gccagtaatt tccgccgccc
     5941 agaccgcgct cttcggcgta ttatcgctct tacctgtcag atactcttca aactgcggat
     6001 acccggtagt gtatttttca aggaacactt tatcgtgttt gccttgtgtc atcagggtat
     6061 gcgcaatccc taacatcagt gccacgtcgg tgcccatatt cggcgcgatc caggtggcat
     6121 tatcgccaaa gaattcgatg gtttcggagc ggataggatc aatggcaatc actggtttgc
     6181 cagatttttt cagctgatgg aagtattcca gcccttgctc atcggtactg ctccaggcaa
     6241 tttttaaggt attcagcggg ttcattcccc acagcaccac aacctggctg ttttccagaa
     6301 tcagcggcca ggaggcctgc tgttcataca cctctacaga accgaccaca tgcggcatga
     6361 tcacctgtgc tgccccggtt gaataatcgc cgctatgccc ggaataaccg cccgccaggt
     6421 tcatataacg ttgcagtaag gtttgcgctt tatgcaacac gccagaagag cgccagccgt
     6481 aagatccggc aaaaatggcc gatggtccgt tagctttacg aatacgctca tgttgctcat
     6541 gaatcagttt taatgcctgt tcccagctca cctgtacata ggtatcttcg ccacgacctt
     6601 tcgccggttg cagtggattg tcgagatagc tttttctcac catcggatgc tgaatacgcg
     6661 ccgtggtgtg tacctgatcc gccgccgtag actgtaagga gttcggtatg gttttcgcca
     6721 gcgcgcctgt cgaagaaaca atcttgccgt ccttcacttc tacgctcatc gctccccaac
     6781 gtcccgcggt gaggatttta ccgcccttct cttctgccca tgcggggagc ggtgctgccg
     6841 atgtcaccac cagcgctcca gcggcaatac cgctgtgttt aataaattca cgtattgtta
     6901 atgccataac ttcctccctg atcaacgagg atcactgttt ctcggtaata tctttggcgt
     6961 tgtactggaa ataccgcgtt aaaatgtcca gttcgttttc gctcatgctg gttcgtgccc
     7021 ccattccttt ggcaatggac ggccacgcat tgacggtgta atggtcggcg gcaatagagg
     7081 catgacaacc agcgcaatag gtatcgtcaa gtttttcagc gtattgccat agcggtttac
     7141 ggtccgctaa tgcgggatcg gtaagcgcac cctgtaaaga cgcctgacgc cattgattgc
     7201 cgtattcgtc agcctgccat tccccgttta ccgtgagcgc cttgatacct tcttcactta
     7261 atgtggctag cgccagccgt tgacctgccg ccaggtagag cgtgttttca ctgccctgca
     7321 tttgataacc ctgcaacaga acgatcggct gtttgccact ggcatcaacg acggtgagat
     7381 cggtaccagg attcacggta gccagctcgc ctatgcgaga agttttgaaa ggataaatat
     7441 gtgcgccatt agtcactgaa gtagcggcct gactttccag ctcatgcgcc gcgttgtcat
     7501 ccatttttat ttctggcgga aaatgggcaa tgcctttatg acaatcgata caggtttcgc
     7561 cgcccttttg tgctttgcta tgcattttct gcgcagattc actttgcgag gcaatatcca
     7621 tggcatcaaa agaatggcaa ctacggcacg ttgcagagtc agttgctttt aattctttcc
     7681 atactgtttc ggccatttcc tggcgatgag tttcgaactt atcgtcactg tctattttgc
     7741 cgctaacaaa ttcatgataa atatctttag atgcttttaa tttagcaaat aaataatcca
     7801 tccctgactt tggaatatgg caatcggcac attccgcacg tatccctttc tggttcgaaa
     7861 agtggacagt tccctgatat tcctcaaaag gtttactcat cgagtggcag gaaaggcaaa
     7921 atgctgtatc cgacgtttta tgtaaggctt tttgcgccag caataacccg ccgccaccaa
     7981 ccacaaccgc tatcagcaaa aacaataacc caatgcgttt tttccctcgc atagattcct
     8041 ctaaataaat tccagaacac cgaaatcatt gtataattaa atacacaacg aattaaccgc
     8101 ttgcataact cggcacaaca ctgcctgaaa taatcgataa agaatatgat ttattacaat
     8161 gtaatcatta attgctaagg aataacccag ttgctattta gaatatatat ttatcagttt
     8221 tagcaattta aatcccataa tcaatgtgaa tataaaaaat atttttagca caacgctatg
     8281 cgcactgtca cccgctcaac agatcacggt tatcgttcgt tttttatact gctcagggat
     8341 aaaaaaaggc ccctgttgaa attgcagggg cctggtacga gcaagcatca tattgggcga
     8401 catgatgcaa cggtaaaaat catttggcct gatggcgttc aatgattcct ttcatttcag
     8461 caaccgccgc cccgtctacg atataacgcg aatactcgtc cgcgtgttca tctgatgaca
     8521 tggacaatcc ttgattacga taacgcatcg gtgaggcttg ccacgctccc gcggagctat
     8581 ggacttccag cactccggca tcgaggaagt ggtgcaggtt ttctgcacgg actcctgctc
     8641 cagccataat gattggagca tcacgatggg caataagttc cataattttt gataaacctt
     8701 gcagtgcgtc tgatttttgc cctgatgtca gtacccgggc aatgcccaat tccgcaagat
     8761 tattgagtgt atataaaggg ttagcgcaca tatcgaaggc gcgatgaaaa gtcactgcca
     8821 gcggaccggc agcagccatt attttttcca ttcgtggcat atcgacattc ccatcaacat
     8881 cgagaacgcc cgtcaccagt ccaggaaaac ctaattcgcg gaccgtgcgc acatcctcaa
     8941 gaatggcggc aaactcaccg tcgctgtagc aaaaatcacc accgcgtggg cgaattatcg
     9001 gatgcacagg gatcgtcacc cgctggcgca cggatttcag tccccccaac gacggcgtta
     9061 agcccccctc ttttggggct gcgcataatt caactctgtc tgcgccgttt tgctgcgccg
     9121 ttagtgcaca ttccatgctg taacagcaaa tttccagtaa tgccattttt gctccttaat
     9181 tacgctgact gctcgctggc aacgatctct tcaatggacc acggatgaaa cttaatagtt
     9241 gttttgccat cggtgaccgc cagcgtcgga ttcggtaatc gctcatgctc accttttggt
     9301 gaactggttt taactgagat ccccggtagg ctgagtcctt catcagaaag caatgccagc
     9361 gcacgggcat tcagcgtttc tggatcaccc ggcagaacga tttcaatatg ttcccaacct
     9421 tcgtgtgggt aacgtttttc cccaggccac ggtagctcca cattggaaaa ctgccagtgc
     9481 gcaacctgta ccggttcatg caatttaaac agacaaatcg gtctgccatt gatcattttt
     9541 tctgacaaaa gctcgccaca ctgttcaaaa ccgcgacgcc agcgctcagc ggtgacgttt
     9601 tgatggcaac gcaaagaaat gtgatcggca gtcagcggag tgatatccaa ccccagacgg
     9661 cgggaaagtt catctaatgc gtgggtaaat cgcggtaaat ccgatgcaat atcctgcagc
     9721 tcgtcgatag attgccagtt cgccataatc actcttcgtc tttcagtaaa agcgttaatt
     9781 taccctgttg ccctgtgcca accaaccgct gatttcacgc cgcttctgat gcaatagtga
     9841 aaacggcaat acgccacgcg cacgttgctg acgaaaacag ccatttgcag tatactccca
     9901 ccctaatttc tttaactggt gcgggcaatt tttgctcgct tcatcaatgt aaggtattcc
     9961 ggtgaatatt caggctcttc tctcagaaaa agtccgtcag gccatgattg cggcaggcgc
    10021 gcctgcggat tgcgaaccgc agattcgtca gtcagcaaaa gttcagttcg gcgactatca
    10081 ggctaacggc atgatggcag ttgctaaaaa actgggtatg gcaccgcgac aattagcaga
    10141 gcaggtgctg actcatctgg atcttaacgg tatcgccagc aaagttgaga tcgccggtcc
    10201 aggctttatc aacattttcc ttgatccggc attcctggct gaacatgttc agcaggcgct
    10261 ggcgtccgat cgtctcggtg ttgctacgcc agaaaaacag accattgtgg ttgactactc
    10321 tgcgccaaac gtggcgaaag agatgcacgt cggtcacctg cgctctacca ttattggtga
    10381 cgcagcagtg cgtagtctgg agttcctcgg tcacaaagtg attcgcgcaa accacgtcgg
    10441 cgactggggc actcagttcg gtatgctgat tgcatggctg gaaaagcagc agcaggaaaa
    10501 cgccggtgaa atggagctgg ctgaccttga aggtttctac cgcgatgcga aaaagcatta
    10561 cgacgaagat gaagagtttg ccgagcgtgc acgtaactac gtggtaaaac tgcaaagcgg
    10621 tgacgaatat ttccgcgaga tgtggcgcaa actggtcgac atcaccatga cgcagaacca
    10681 aatcacttac gatcgtctca acgtgacgct gacccgtgat gacgtgatgg gcgaaagcct
    10741 ctacaaccca atgctgccag gaattgtggc ggatctcaaa gccaaaggtc tggcagtaga
    10801 aagcgaaggg gcgaccgtcg tattccttga tgagtttaaa aacaaggaag gcgaaccgat
    10861 gggcgtgatc attcagaaga aagatggcgg ctatctctac accaccactg atatcgcctg
    10921 tgcgaaatat cgttatgaaa cactgcatgc cgatcgcgtg ctgtattaca tcgactcccg
    10981 tcagcatcaa cacctgatgc aggcatgggc gatcgtccgt aaagcaggct atgtaccgga
    11041 atccgtaccg ctggaacacc acatgttcgg catgatgctg ggtaaagacg gcaaaccgtt
    11101 caaaacccgc gcgggtggta cagtgaaact ggcagacctg ctggatgaag ccctggagcg
    11161 tgcacgtcgt ctggtggcag aaaagaaccc ggatatgcca gctgacgagc tggaaaaact
    11221 ggctaacgcg gttggtattg gtgcggtgaa atatgcggat ctctccaaaa accgcaccac
    11281 ggactacatc ttcgactggg acaacatgct ggcgtttgag ggtaataccg cgccatacat
    11341 gcagtatgct tacacgcgtg tattgtccgt gttccgtaaa gcagaaatta acgaagagca
    11401 actggctgca gctccggtaa tcatccgtga agatcgtgaa gcgcaactgg cagctcgcct
    11461 gctgcagttt gaagaaaccc tcaccgtggt tgcccgtgaa ggcacgccgc atgtaatgtg
    11521 tgcttacctg tacgatctgg ccggtctgtt ctctggcttc tacgagcact gcccgatcct
    11581 cagcgcagaa aacgaagaag tgcgtaacag ccgtctgaaa ctggcacaac tgacagcgaa
    11641 gacgctgaag ctgggtctgg atacgctggg tattgagact gtagagcgta tgtaatcgat
    11701 ttttcgtgag agtgaagcct gatcagggtt agccgatcag gcttttttat tgccatctaa
    11761 atgtattctg aaaacggaca tgccattgtt ttctcactgt tgagtaagag gccagaagcg
    11821 taatatccag gcccaggaaa acgataactg ttcaatttaa ggaataccga agtgtttaaa
    11881 tttcttgtat taacattagg cattatctct tgccaggctt acgcagaaga tacggttata
    11941 gtaaacaacc atgacacttc agccatcaaa gattgttggc aaaaaaattc agatgatgat
    12001 actgacatta acgtgatcaa atcatgcctg cgacaagaat acaatctcgt cgatacgcaa
    12061 ttaaataaag cctatggtga agcttatcgt tatatagaac aagtgccacg cacaggtgca
    12121 aaaaaacttg atactgaaca acttaacttg cttaaaaaat caggtaatga ctccaactta
    12181 ctgatagtgt tttatgttca gataatgccc gatgaccttg tcatgcagct ccaccgattt
    12241 tgagaacgac agtgacttcc gtcccagcct tgccagatgt tgtctcagat tcagattatg
    12301 tcgctcaatg cgctg
//
LOCUS       DAETPZ010000108        12233 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.108, whole genome shotgun sequence.
ACCESSION   DAETPZ010000108 DAETPZ010000000
VERSION     DAETPZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.108"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..494)
                     /locus_tag="K0W59_003446"
     CDS             complement(<1..494)
                     /locus_tag="K0W59_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3024342.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(525..1217)
                     /gene="fimC"
                     /locus_tag="K0W59_003447"
     CDS             complement(525..1217)
                     /gene="fimC"
                     /locus_tag="K0W59_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HBI3024343.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1418..1960)
                     /gene="fimA"
                     /locus_tag="K0W59_003448"
     CDS             complement(1418..1960)
                     /gene="fimA"
                     /locus_tag="K0W59_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBI3024344.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2441..3307
                     /gene="folD"
                     /locus_tag="K0W59_003449"
     CDS             2441..3307
                     /gene="folD"
                     /locus_tag="K0W59_003449"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HBI3024345.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3309..3521
                     /gene="ybcJ"
                     /locus_tag="K0W59_003450"
     CDS             3309..3521
                     /gene="ybcJ"
                     /locus_tag="K0W59_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HBI3024346.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3629..4150
                     /locus_tag="K0W59_003451"
     CDS             3629..4150
                     /locus_tag="K0W59_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBI3024347.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(4186..5571)
                     /gene="cysS"
                     /locus_tag="K0W59_003452"
     CDS             complement(4186..5571)
                     /gene="cysS"
                     /locus_tag="K0W59_003452"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBI3024348.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5745..6239
                     /gene="ppiB"
                     /locus_tag="K0W59_003453"
     CDS             5745..6239
                     /gene="ppiB"
                     /locus_tag="K0W59_003453"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HBI3024349.1"
                     /translation="MVTFHTNHGDIVIKTFDNKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6242..6964
                     /gene="lpxH"
                     /locus_tag="K0W59_003454"
     CDS             6242..6964
                     /gene="lpxH"
                     /locus_tag="K0W59_003454"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HBI3024350.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7082..7591
                     /gene="purE"
                     /locus_tag="K0W59_003455"
     CDS             7082..7591
                     /gene="purE"
                     /locus_tag="K0W59_003455"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBI3024351.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7588..8655
                     /gene="purK"
                     /locus_tag="K0W59_003456"
     CDS             7588..8655
                     /gene="purK"
                     /locus_tag="K0W59_003456"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBI3024352.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8792..9685)
                     /locus_tag="K0W59_003457"
     CDS             complement(8792..9685)
                     /locus_tag="K0W59_003457"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBI3024353.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9682..10329)
                     /locus_tag="K0W59_003458"
                     /pseudo
     CDS             complement(9682..10329)
                     /locus_tag="K0W59_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10332..11564
                     /gene="allC"
                     /locus_tag="K0W59_003459"
     CDS             10332..11564
                     /gene="allC"
                     /locus_tag="K0W59_003459"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HBI3024354.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11575..>12233
                     /gene="allE"
                     /locus_tag="K0W59_003460"
     CDS             11575..>12233
                     /gene="allE"
                     /locus_tag="K0W59_003460"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HBI3024355.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     W"
BASE COUNT         2966 a         3168 c         3121 g         2978 t
ORIGIN      
        1 tgagcgctac tcaacaacgc tatctgagga atagtgatgt tcaggcgcat cgctgcaaaa
       61 tcaaagttaa ttgtggcatc aggtacagct ttcagtaaat tgatgcattt attgttttgc
      121 tgctgggcta actcgggaaa tgcactgcta ttaaggccaa ttcgttcaag gagtacctgg
      181 ttaaaacagg gcattaaccc acctgattta tcacctgtat tttctgttgt cgattcaaat
      241 acgatatcct gtgaaccaat gaactcatca ttacgccaga gatccacccg ataaacgccc
      301 gcaggttgat gattacctct ctcaaagcgc gataaatcag caacggatgt gccattctct
      361 aataaaaatg ccgggttgaa ataactttcc gcatagctta aaaaggaggg taaaatggca
      421 agagatgaat aacaaattcc ggccagacac caggtatacc tctgcggaat atccgtagta
      481 gtgggtattt tcataattag ctctgatatt gtcagatgat tcggttagtt taagttcact
      541 tctctgaccg gggttactga accataatca ttaacggtct gaaatttcag ctttccctgc
      601 actcctgagg gtaagggaat ttgtgaacta actctgggtg ccgccatagc attccccaac
      661 ttttggctgc ccacttgcag gttaacgagg gtgacataaa aaggtgatgg attatgaaca
      721 ttgatatagt taccggatcg cgaaaacttg agtgtgtccg gcgcttctgc gggtaattct
      781 tgtaattgaa ttggacggag aaataatttc atgcgcgata aaatcgccag ttgcaaaaca
      841 ttcctgccgt tcaatgcatt tttatctacc gaagggatcg tcttaacatt catccagaac
      901 agagactcac gatctgccgc cagcggtgga ccggtgtaaa taatacgcaa agtattttcg
      961 cttttgggtt cactaacaaa tagtggcggt gtaatgatga atgacttttc ttttacaccg
     1021 ctgctgtttt caatccacga attgaccaga aagagctcat tggtatggct atttttaatc
     1081 cataccgcag tctgtttagc atcagcggga taaataatac gcgtggcacc taatgcgatc
     1141 cctccggcag catgggcgct ggccgaaatg attaaataaa atatcatgag catcaataac
     1201 tttattttag tcatcatagt taatacgcct tgtgtattcc tgacggcaaa agattgcttt
     1261 ggcttcttac aacacatcat tcccttttca tttaataaag cggcatgata caaagtgaac
     1321 gtggtttcga tttacattgc caccataccg cttcagacaa ccggtacatt actttgttat
     1381 aagacgtgat atataattca aaacaacgtg gttttgatta ttcgtatctc ataataaaag
     1441 tcgcgtcagc atttgcttgc cctgctgatg cacttgtacc ggtgccttta taacgtgcag
     1501 aaaaatgaag aacgttggtc ccggggatca agttctggtt ggttgagaag ctattcccat
     1561 caggtttgag aattgcggat gtattatcaa gtatttcaat accgacaccc gttgctgttg
     1621 ttgtatttgt actggatgca atggccaata aattatcatt gattgcatca gcctgaccag
     1681 aaaatgcaac ggcagcatta gcggcaacaa caggatcgca gtcgttcaac tgaatggtga
     1741 atggaattaa tgcagaggta ttaccaacag cattgaaaat atcggtacga tattgaccga
     1801 gtgtgacaac ctggtctgcc gagtcagtat tcactgaaca ggcagcattc accagttcgc
     1861 cttcaaagtg aattgtaccg ccatctacaa cggcagcata actacccgta gcggcgaata
     1921 gtgcggcagc cagcgcagac gaaataaatc ttaatttcat atatattcct tcaatctcat
     1981 ttatcgactc cacatccgta tataaccgat tactttattt aagacactga tagtagtaaa
     2041 attccttttt atcctctaag aatgtcttaa ttgaaaatat gcactctatt ctaaaaaata
     2101 gagagccccg ttagatgaat acttccgcgc aaaatatatt caacacaaat atagacctga
     2161 agcggtaaat taccaggctg aaaattcttt ttatattgtc aggtatttct taaattatct
     2221 taatccttaa acaaggaaat aaatcagttc cagatttaca acgccatcat gtacgaaaaa
     2281 tgaagctttc aatctcagcg acctcagcga cggtgcgcct aaccttcgca agaggtcgct
     2341 tcacgcgata aatctgaaac gaaacctgac agcgcgcccc gcttctgaca aaatagacgc
     2401 atccccttcg acctacgtaa cagatggaat cctctctctg atggcagcaa agattattga
     2461 cggtaaaacg attgcgcagc aggtgcgctc tgaagttgct caaaaagttc aggctcgtat
     2521 tgcagccgga ctgcgggcac caggactggc cgttgtgctg gtgggtagta accctgcatc
     2581 gcaaatttat gtcgcaagca aacgcaaggc ttgtgaagaa gtcgggttcg tctcccgctc
     2641 ttatgacctc ccggaaacca ccagcgaagc ggagctgctg gagcttatcg atgcgctgaa
     2701 tgccgacaac accatcgatg gcattctggt tcaactgccg ttaccggcgg gtattgataa
     2761 cgtcaaagtg ctggaacgta ttcatccgga caaagacgtg gacggtttcc atccttacaa
     2821 cgtcggtcgt ctgtgccagc gcgcgccgcg tctgcgtccc tgcaccccgc gcggtatcgt
     2881 cacgctgctt gagcgttaca acattgatac cttcggcctc aacgccgtgg tgattggcgc
     2941 atcgaatatc gtcggtcgcc caatgagcat ggaactgttg ctcgcaggtt gcaccaccac
     3001 cgtgactcac cgcttcacta aaaatctgcg tcatcacgta gaaaatgccg atctgttgat
     3061 cgttgccgtt ggcaagccag gctttattcc cggtgactgg atcaaagaag gcgcaattgt
     3121 gattgatgtc ggcatcaacc gtctggaaaa tggcaaagtt gtgggcgacg tcgtgtttga
     3181 agacgcggct aaacgcgcct catacattac gcctgttccc ggcggcgttg gcccgatgac
     3241 ggttgccacg ctgattgaaa acacgctaca ggcgtgcgtt gaatatcatg atccacaggg
     3301 tgagtaacat ggcgacattt tctttaggta aacatccgca cgttgagctg tgcgacttgc
     3361 tgaaactgga aggctggagc gaaagcggcg cgcaggcgaa aatcgcgatt gccgaaggcc
     3421 aggtgaaagt cgacggtgcg gttgaaacgc gcaaacgctg caaaatcgtc gccggtcaga
     3481 cagtgagttt tgcaggacac agcgtacagg ttgttgcctg atccctttcc cggcctgtat
     3541 caacaggccg gttctctttt cagacttttg ccctctacgc cccgttcatc catgctgata
     3601 gcatcgacat tttgccgaag cgaccatcat gccaaccgtt attacgcacg ccgccgttcc
     3661 cctttgcatt ggtttagggt tgggttcgaa agtcatccct ccgcgtctgt tatttgccgg
     3721 aatcatccta gcgatgctgc ccgatgccga cgtattgtcg tttaaatttg gcgttgctta
     3781 cggcaatgtt tttggtcatc gcgggtttac ccattcgctg gtgtttgcgt ttgttgtccc
     3841 gctcttatgt gtgctgattg gacgtcgatg gttcagggct gggctgattc gctgctggct
     3901 gtttttaacc gtctcgttgc tatcgcacag cttactggat tcggtaacca ctggcggtaa
     3961 gggcgttggc tggctgtggc cgtggtcaga tgaacgcttt ttcgctccct ggcaggtgat
     4021 taaagtcgcg ccgtttgcat tgtctcgtta caccacagcg tacgggcatc aggtgattat
     4081 ttccgaattg atgtgggtgt ggttgccggg gatgctactg atgggaatgt tgtggtggcg
     4141 cagacgataa ccggatgcga aaactcgcat ccggcaattg cgcaattact tacgacgcca
     4201 ggtggtccct tgcgggccat cttccagcac gatccccatc tcgttaagac ggtcacgtgc
     4261 cgcatctgcc gccgcccagt ctttcgcttt acgggcatcc agacgctgtt gaattaacgc
     4321 ttcaatctca gccacttcgc tgtcgtctgc ctgcgcgccg ctttgcagga acgcttccgg
     4381 ttcttgctcc agcaggccca atacagcgga aagtttacgc aggtgagatg ccattgcatt
     4441 cgccgctgcc atatcttctg ctttcagacg gttcacttca cgcgccatat caaacagcac
     4501 ggaataggct tccggggtgt tgaaatcgtc gtccatcgct tcaataaagc gcgcttcaaa
     4561 cgcttcgcca ccggcaggcg caacggtttt atctgtgccg cgcagcgcag tgtagaggcg
     4621 ctccagcgcc gcacgcgcct gcttcaggtt ctcttcgctg tagttcagct ggctgcgata
     4681 gtggcccgac atcaggaagt aacgcacggt ttccgcgtcg tagtatttca gcacatcgcg
     4741 cacggtaaag aagttaccca gcgatttgga catcttctcg cggtcaacca tcaccattcc
     4801 ggagtgcatc cagtagttca catactgacc atcatgggca caggtggact gcgcgatttc
     4861 gttctcgtgg tgcgggaaca tcaggtctga accgccgccg tggatatcaa agtggttacc
     4921 cagctgcttg cagttcatgg ccgaacattc aatgtgccag ccaggacgac ccgcgcccca
     4981 cggagacggc cagctcggtt cgccctcttt cgacatcttc cacagaacga agtccatcgg
     5041 gttgcgtttg tcatcaacca cgtcaacgcg cgcgccagcc tgcagctggt cgagatcctg
     5101 acgcgacagc acgccataag ttggatcggt cgggacgtcg aacatcacgt cgccgttgtc
     5161 cgccacataa gcgtgacctt tggcgatcag ttgttcagtg agttcaataa tttctgcgat
     5221 atggtgcgtc gcgcgcggct ccatatccgg gcgcagaatg ttcaaagcat caaaatcttt
     5281 gtgcatttcg gcgatcatgc gatccaccag cgccacaaag ctttcgccat tttcattggc
     5341 gcgtttgatg attttgtcgt cgatatcggt aatgttgcgc acatacttca gtttatagcc
     5401 gaggaaacgc agatagcgcg caaccacgtc aaaagcaaca aaggtacgcc cgtgaccgat
     5461 atgacagaga tcgtaaacgg tgattccaca cacgtacatg ccgacttccc cggcgtgaat
     5521 aggcttaaat tcctcttttt ggcgtgtcag agtattgaag atttttagca tcgaagattc
     5581 cgtttagaca tgtgtgggta attgagttgc gtataatacc catatttccc gcgcgaatca
     5641 gcatacattg cgtgatgatc cgatcgtgcg gttatgctat aacaccaccc tatatatgac
     5701 ccgaactggg ttgaagcacc aatcaaacgg aacaggatgc aaaaatggtt actttccaca
     5761 ccaatcacgg cgatattgtc atcaaaactt ttgacaataa agcacctgaa acagttaaaa
     5821 acttcctgga ctactgccgc gaaggttttt acaacaacac cattttccac cgtgttatca
     5881 acggctttat gattcagggc ggcggttttg aaccgggcat gaaacaaaaa gccaccaaag
     5941 aaccgatcaa aaacgaagcc aacaacggcc tgaaaaatac ccgtggtacg ctggcaatgg
     6001 cacgtactca ggctccgcac tctgcaactg cacagttctt catcaacgtg gttgataacg
     6061 acttcctgaa cttctctggc gaaagcctgc aaggttgggg ctactgcgtg tttgctgaag
     6121 tggttgacgg catggacgtg gtagacaaaa tcaaaggtgt tgcaaccggt cgtaacggta
     6181 tgcaccagga cgtgccaaaa gaagacgtta tcattgaaag cgtgaccgtt agcgagtaat
     6241 cgtggcgaca ctctttattg cagatcttca tctctgcgtg gaagaaccgg cgatcaccgc
     6301 cggttttctg cgttttttag cgggggaagc ccgcaaggcc gacgcgctgt atattcttgg
     6361 cgatctgttt gaagcatgga ttggcgacga cgatcccaac ccactccatc gccagatggc
     6421 ggcagcgatc aaagcggtgt ccgattccgg cgttccctgt tatttcattc atggcaaccg
     6481 tgattttctg ctcggcaaac gctttgcccg tgaaagcggc atgacattat tgccggaaga
     6541 aaaggtgctc gaactttatg gtcgccgggt gttgattatg catggcgaca cgctgtgcac
     6601 cgatgacgcg ggttatcagg cttttcgcgc caaagtccac aaaccctggc tgcaaatgct
     6661 attcctcgcc ctgccgttgt ttgtgcgcaa acgcattgcc gcgcgaatgc gcgcgaacag
     6721 caaagaagcc aacagcagta aatcgctggc gatcatggac gttaaccaaa acgctgtggt
     6781 cagtgcgatg gaaaaacatc aggtgcaatg gctaatccac gggcataccc atcgcccggc
     6841 ggtgcatgaa cttatcgcca accagcaacc tgcttttcgc gtggtactgg gtgcctggca
     6901 tacggaaggt tcgatggtga aagtcacggc ggatgacgtt gagctgattc attttccgtt
     6961 ttaaaaaacc cacaactttg ctgatttcac agccacgcaa ccgttttcct tgctctcttt
     7021 ccgtgctatt ctctgtgccc tctaaagccg agagttgtgc accacaggag ttttaagacg
     7081 catgtcttcc cgcaataatc cggcgcgtgt cgccatcgtg atggggtcca aaagcgactg
     7141 ggctaccatg cagttcgccg tcgaaatctt cgaaatcctg aatgtcccgc accacgttga
     7201 agtggtttct gctcaccgca cccccgataa actgttcagc ttcgccgaaa gcgccgaaga
     7261 gaacggttat caggtgatta ttgcgggcgc aggcggcgca gcgcacctgc caggcatgat
     7321 tgccgccaaa acgctggtgc cggtgctggg cgtgccagta cagagcgccg cactgagcgg
     7381 tgtcgatagc ctctactcca tcgtacaaat gccgcgcggc attccggtgg gtacgctggc
     7441 gattggtaaa gctggcgcgg caaacgcggc attactggca gcacaaattc tcgcgactca
     7501 cgataaagag ctacaccagc gtctgaatgg ctggcgcaaa gcccagaccg acgaagtgct
     7561 ggaaaacccg gacccgcgag gtgcggcatg aaacaggttt gcgtcctcgg taacgggcag
     7621 ttaggccgta tgctgcgtca ggcaggcgaa ccgttaggca ttgctgtctg gccggtcggg
     7681 ctggacgctg aaccggcggc ggtgcctttt caacaaagcg tgattaccgc tgagatcgaa
     7741 cgctggccgg aaaccgcatt aacccgcgag ctggcgcgtc atccggcctt tgtgaaccgc
     7801 gatgtgttcc cgattattgc tgaccgtctg actcagaaac agcttttcga taagctccac
     7861 ctgccgaccg cgccgtggca gttacttgcc gatcgcagcg agtggcctgc ggtgtttgat
     7921 cgtttaggtg aactggcgat tgttaagcgt cgcactggtg gctatgacgg tcgcggtcaa
     7981 tggagtttac gcgccaatga aaccgaacag ttaccggcag agtgttacgg tgaatgtatt
     8041 gtcgagcagg gcattaactt ctctggtgaa gtatcgctgg ttggcgcgcg cggctttgat
     8101 ggcagcaccg tgttttatcc gctgacgcat aacctgcatc aggacggtat tttgcgcacc
     8161 agcgtcgctt ttccgcaggc caacgcacag cagcaggcgc aagccgaaga gatgctgtcg
     8221 gcgattatgc aggagctggg ctatgtgggc gtgatggcga tggagtgttt tgtcaccccg
     8281 caaggtctgc tgatcaacga actggctccg cgtgtgcata acagcggtca ctggacacaa
     8341 aacggtgcca gcatcagcca gtttgagctg catctgcggg cgattaccga tctgccgtta
     8401 ccgcaaccag tagtgaataa tccgtcggtg atgatcaatc tgattggtag cgatgtgaat
     8461 tatgactggc tgaaattgcc gctggtgcat ctgcactggt acgacaaaga agtccgtccg
     8521 gggcgtaaag tggggcatct gaatttgacc gacagcgaca catcgcgtct gactgcgacg
     8581 ctggaagcct taatcccgct gctgccgcca gagtatgcca gcggcgtgat ttgggcgcag
     8641 agtaagttca gttaagcagg tgctcatttg attgccggat gcggcgtaaa cgccttatcc
     8701 ggcttaccgc tccggtaccc attgtaggcc tgataagacg cgtcaagcgt cgcatcaggc
     8761 atggtgcacc gattgccgga tgctctcatc actacagcga aatacaggtc cccgcttcgc
     8821 ccgccagcgt ctcttcaatt cgcgataacg ccccaatcca cgcgggttta ctacggcttc
     8881 tgacataacc actcaccgcc gttaccttcg gccccatcga accatcggct ttggcaaatg
     8941 gcgctaactc atccggtgtg gcatggcgaa tggcacgttg ctgcggcgtt ccccagtttt
     9001 catataccgc atcagcatcg gtgaggatca ccagtccatc ggcattaatc tgctcggcga
     9061 gcaacgcagc ggcgagatct ttatcaatca ccgcttcact ccctgcccca tcttccgtca
     9121 caggcacacc gccgccgcca ctgcaaatca ccacatgccc ctctttgagc aacaactcga
     9181 tggcttcgct gtcgagaatt ttacgcggtt gcggagacgc caccacccgg cgcaaatatt
     9241 taccatcacg tttcatctgc cagccgtaag ccgcttccag tgcctcttgt tcttctggct
     9301 gataaaccgg accaataaat ttctctggct gcaaaaacgc cggatcatca ggcgaaacct
     9361 caatgcgcgt cagcaccgtc gtaacaggcg gcatctgcgg ctgtgcgctc aagctctgcg
     9421 ccagcatata gccaatcatc ccctggcttt ccgccaccag cacatccagc ggatacggtt
     9481 ctacctcttt ccacgccaga ttctgtaacg ccagcagccc cacctgcggc ccgttgccgt
     9541 gaacaatcgc cagccgataa gaacgggcca ggcgtgccag cgcgggtaca gcactggcga
     9601 tattgcgata ttgattttcc gccgtcagcg cctcaccgcg ctggagtaag gcgttgcccc
     9661 caagagctac aaccagtgtt ttcatggttt tccttgtaat aattgttggc caagccagaa
     9721 ccccagcagc gtatctgccc ctgacgtatg ccccagcgcc agcagcgaat caatcgcaac
     9781 agcggtacgt ttcgggcaac tcagagcatg gacaaagtgc aggagtggcg aggcgaaata
     9841 tccttgcgcg gcataacgta aataactgac gctcaccgct gtggtaacga gttgaagatt
     9901 gtcggaacag gcaaaaaacg ggcgaccggc gcgcgcatct aaagcgccat aataccaggc
     9961 cgccagcagc attccgctca acgtgtcatc atgacttggc gttaatccgg ggcctttacc
    10021 cagccagtgc cgccagtcgg tcttaacgcc attgagcgcg gcctgaaaac agtgacgaaa
    10081 ctggcgtaat tcagcgggca gcggatcgct tgccgccatc gccagtggcc cgaaaagtcc
    10141 ggtttcctcc gcgcgttgca tccatgcagc tgcaagcggt tgaggatgcg aaggcggcgt
    10201 aatacgcagc aaacaataac gctgtggctg tttaaccgtg aaacgcccga ggcgaatccc
    10261 ttgagccaca acctgtggtc gttcattgcc gcataatcca ccgcataacg catcgaattg
    10321 cgtgcggcga aattacacat tcccgtcaag ctatagaaga aacgctgccc tggctttcct
    10381 cttttggcgc tgacccaacg ggtgggatga cccgtttact ttattcgccg gaatggctgg
    10441 aaacccagca gcaatttaaa aaaagaatgg cagcaagtgg gctggaaaca cgtttcgatg
    10501 aagtggggaa tttatacggt cgcctgagtg gcaccgaata tccacaggaa gtggttctga
    10561 gcggttcgca tatcgatacc gtggttaacg gcggtaacct cgacgggcaa ttcggcgcgc
    10621 tggcggcgtg gctggcaatt gactggttga aaacgcaata cggcgcaccg ttacgtacgg
    10681 tcgaagtggt ggcgatggca gaagaagaag gcagccgctt cccgtatgtc ttctggggca
    10741 gtaaaaatat ctttgggctg gcgaatcctg acgacgtgcg gaatatctgt gatgccaaag
    10801 gaaatagttt tgccgatgcg atgaaggctt gcggatttac tctgcctaac gccccactaa
    10861 ctccgcgtca ggatataaaa gcctttgttg aactgcatat tgaacagggc tgtgtgctgg
    10921 aaagtaatgg gcaatcaatt ggcgtggtga atgcaattgt cgggcagcgt cgctatacgg
    10981 tgacgctgaa cggcgaatca aaccatgcag gcaccacgcc gatgggttat cgtcgtgata
    11041 cggtttacgc tttcagtcgc atttgccatc agtcgatcga aaaagcgaaa aagatgggcg
    11101 atccgctggt tctgaccttt ggcaaagtag agccgcgccc gaatacggta aatgtggtgc
    11161 cgggtaaaac cacgttcacc attgattgtc gtcataccga cgctgccgtg ctgcgcgatt
    11221 tcacccaaca gttagaaaac gacatgcggg cgatttgcga tgaaatggac attggtattg
    11281 atatcgattt atggatggac gaagaacccg tgccgatgaa taaggagctg gtcgccaccc
    11341 tgacagaatt gtgtgaaaga gaaaaactga attaccgggt gatgcacagt ggtgccgggc
    11401 acgacgcgca aattttcgcg cctcgcgtac caacctgcat gatttttatc cccagcatca
    11461 acgggatcag ccataacccg gcggaacgca ccaatattac tgaccttgcc gaaggggtca
    11521 aaacgttggc actcatgctt tatcaacttg cctggcagaa ataaggagtc ataaatggga
    11581 tatttaaata acgtcaccgg ttaccgcgaa gatttactgg ctaaccgtgc gattgttaaa
    11641 cacggtaatt tcgcactgtt aaccccggat ggtctggtaa aaaatattat tccgggcttt
    11701 gaaaattgtg acgcgacaat cctctccacg ccaaagctgg gtgcctcttt tgttgattat
    11761 ctggtcacac tgcatcaaaa cggtggcaac caacagggct tcggtggcga aggcattgaa
    11821 acgttcctgt atgtgatctc tggaaatatc actgccaaag ccgaaggcaa aacatttgcc
    11881 ttaagcgaag gtggctatct ttattgccca ccaggctcct taatgacgtt tgttaacgcc
    11941 caggccgaag acagccaaat ctttttatat aagcgccgct atgttccggt agaaggccat
    12001 gcaccgtggc tggtttctgg caatgccagc gaactggaac gcattcacta tgaaggtatg
    12061 gatgatgtga tcctgctgga ttttctgccc aaagagttag gttttgatat gaacatgcat
    12121 atcctctctt ttgcaccagg tgccagccac ggttatatcg aaacacacgt tcaggaacat
    12181 ggtgcctata ttctttccgg tcagggggtt tataacctcg acaataactg gat
//
LOCUS       DAETPZ010000109        12153 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.109, whole genome shotgun sequence.
ACCESSION   DAETPZ010000109 DAETPZ010000000
VERSION     DAETPZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.109"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            128..997
                     /locus_tag="K0W59_003461"
     CDS             128..997
                     /locus_tag="K0W59_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HBI3024356.1"
                     /translation="MAISIKGVNTGVIRKGNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1200..1553)
                     /locus_tag="K0W59_003462"
     CDS             complement(1200..1553)
                     /locus_tag="K0W59_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HBI3024357.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1691..3337)
                     /gene="groL"
                     /locus_tag="K0W59_003463"
     CDS             complement(1691..3337)
                     /gene="groL"
                     /locus_tag="K0W59_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBI3024358.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3381..3674)
                     /locus_tag="K0W59_003464"
     CDS             complement(3381..3674)
                     /locus_tag="K0W59_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBI3024359.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3950..5206
                     /gene="yjeH"
                     /locus_tag="K0W59_003465"
     CDS             3950..5206
                     /gene="yjeH"
                     /locus_tag="K0W59_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HBI3024360.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLEALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5222..5698)
                     /gene="fxsA"
                     /locus_tag="K0W59_003466"
     CDS             complement(5222..5698)
                     /gene="fxsA"
                     /locus_tag="K0W59_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HBI3024361.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6035..7471
                     /gene="aspA"
                     /locus_tag="K0W59_003467"
     CDS             6035..7471
                     /gene="aspA"
                     /locus_tag="K0W59_003467"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HBI3024362.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7589..8890
                     /gene="dcuA"
                     /locus_tag="K0W59_003468"
     CDS             7589..8890
                     /gene="dcuA"
                     /locus_tag="K0W59_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HBI3024363.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9006..9344
                     /gene="cutA"
                     /locus_tag="K0W59_003469"
     CDS             9006..9344
                     /gene="cutA"
                     /locus_tag="K0W59_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HBI3024364.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9320..11017
                     /gene="dsbD"
                     /locus_tag="K0W59_003470"
     CDS             9320..11017
                     /gene="dsbD"
                     /locus_tag="K0W59_003470"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HBI3024365.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTIDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11054..11629
                     /locus_tag="K0W59_003471"
     CDS             11054..11629
                     /locus_tag="K0W59_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI3024366.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11736..11811
                     /locus_tag="K0W59_003472"
     tRNA            11736..11811
                     /locus_tag="K0W59_003472"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11769..11771,aa:Phe,seq:gaa)
BASE COUNT         2924 a         3134 c         2989 g         3106 t
ORIGIN      
        1 ttttctgttt atatagagtg agcacggcta tttatgttat tttaacaact attactacca
       61 agcccattta tctggcttat aaattaaaat taattattta attgagaaat ggttagggag
      121 aacctacatg gccataagca tcaagggtgt taataccggt gttattcgca agggtaataa
      181 tttcattgca ctggcgctga aaattaaaga acctcgcaat aaagaatcgt tgttctttat
      241 gtctgtcatg gaactgcgcg acctgctgat tgcgctggaa agtcgtctgc atcaaaagca
      301 taaactggat gctgctgcac gccttcagta tgagcaagct cgcgataaag tcattaagaa
      361 aatggcagaa aacatcccag aaattctggt tgatgagctt aaaaatgccg atattaatcg
      421 tcgggttaac acgctggaac ttaccgataa tcaaggcgaa aatcttacct tcgtattaac
      481 gctacatgac ggtagcaaat gtgagttagt cgtcaatgag ttgcagattg aaatgctggc
      541 gcgagcaatc attcatgcaa ttaataatgc ggaaatgcgt gaactggcat tacgtattac
      601 ctcactgtta gatttcctgc cgttatacga cgttgactgc caggacaatg gcaatctgga
      661 atatgacacc tattcccaac cggaatggaa acataacttg ttcgaccatt atctggcggt
      721 gctttaccgc tttaaagatg aaagtggcaa agaacaattt agcggcgcag tggtgaaaac
      781 gcgtgaagca acgccgggta aagaaataga ggcgatcact cgccgaatgc ttgatttcag
      841 cccacgattg aaaaaactcg cgggtgtgcc atgtcaggtc tatgttcgca ccgtagcggc
      901 gaataacgcc caaccgctaa cccaggatca atgcttacgc gcgctgcatc acttacgtgt
      961 acaatccacc agcaaaactg caccacaggc gaaataatct catctttgct aatgcgaggc
     1021 tggagccatc cagcctgaaa tgactgataa cgtgatcgtt ggtcatgccg gatgcggcgt
     1081 aaacgcctta tccggcctac aaattctcaa gcattcaatc agttgcaatg cctgctgtag
     1141 gcctgataag cgaagcgcat caggcaataa tacgtttgtc acccagcctc tggatcacat
     1201 cagttcgggc acttataaac ctgaccgata atctggctat ccgtcgggac aaaactggac
     1261 aacattccct gcgacgggct gctgatgcca taaatcacgt taccgcccat tgcagccgcc
     1321 tggttgcgca gatcgtttgc tgcgccgcgc atagaaccgc cctcttctcc gtgttgcccg
     1381 gaaagccagt tgctttgctt acctgtcgca gtaccaatca gctggcactc tgcgcctggc
     1441 tgctcgtcca caatgcgtac actctgaccg gcagcactca attcgttgct ggagctacaa
     1501 cccgccatca gtagcgcggc accgacaatc cctgctaagt attttacgtg catgttattc
     1561 cccataatca atgagctgga cgccactcgc gtcgtccgtg tctgaatctt atactagaaa
     1621 gatgaccaaa agaaaaaccc ccagacattt ctgcccgggg gtttgtttat ttctgcgaag
     1681 tgcagggcaa ttacatcatg ccgcccatgc cacccatgcc gcccataccg ccagcagcgc
     1741 ctaagtcagc tgcatcgttt ttcggcaggt cggtaaccat gcattcggtg gtgatcatca
     1801 ggccagccac agaagctgcg tactgcagag cagaacgggt tactttggtt gggtccagga
     1861 tacccatgtc gatcatgttg ccgtattctt cggttgctgc gttgtaaccg tagttgccgt
     1921 cgccgccttt aacggtgtta gcaacaacag acggttcttc gccgcagttc aggacgatct
     1981 gacgcagcgg agcttccatt gcacgcagtg caactttgat acccacgttc tggtcttcgt
     2041 tctgaccacg caggtcagcc agtttagacg ctacgcggat cagcgcaaca ccaccaccag
     2101 caaccacgcc ttcttctacc gcagcacggg tcgcgtgcag ggcatcttca acgcgtgctt
     2161 ttttctcttt catttcaact tcggtagcag cacctacttt gataactgca acgccgcctg
     2221 ccagtttcgc tacgcgctcc tgcagttttt cacggtcgta gtcagaagtt gcttcttcaa
     2281 tctgctgacg gatctgagca acacggccct ggattgcagc ttcttcaccc acgccatcga
     2341 tgatagtggt ggtgtctttg ttgatcacaa cacgtttagc ctgacccagg tcttccaggg
     2401 ttgctttttc cagctccata ccgatctctt cagagattac ggtaccgcca gtcagggttg
     2461 cgatatcctg cagcatagct ttacgacgat cgccgaagcc cggagcttta accgcagcaa
     2521 ctttcacgat gccacgcatg gtgttaacaa ccagagttgc tagcgcttcg ccttcaacat
     2581 cttcagcgat gatcagcagc ggtttgcctg ctttggcaac agcttccaga accggcagca
     2641 tttcgcggat gttggagatt ttcttgtcag ccagcaggat gaacgggctt tccagttcta
     2701 ctgcgccagt ttccggcttg ttgatgaagt aaggagacag gtagccacgg tcgaactgca
     2761 taccttcaac cacgtccagt tcgtcctgca gaccggtacc gtcttcaacg gtgataacgc
     2821 cttctttacc gactttgtcc atcgcttcag cgatcagttt acctacggtt tcgtcggagt
     2881 tagcggagat ggtaccaacc tgagcaatcg ctttagaatc agagcacggt acggacagcg
     2941 ctttcagttc ttcaactgca gcggtaaccg ctttgtcgat accacgtttc aggtccatcg
     3001 ggttcatgcc cgcagcaaca gctttcagac cttcagtgat gatagcctga gccagtacgg
     3061 ttgcagtggt ggtaccgtcg cctgcagcgt cgttcgcttt ggaggcaact tctttcacca
     3121 tctgcgcacc catattttcg aacttgtctt ccagttcgat ttcacgagca acggaaacac
     3181 catctttggt gatggtcggt gcaccgaaag atttatccag aactacgtta cggccttttg
     3241 gaccgagggt aactttcact gcatctgcca gtacgtttac gccgcgcagc attttcacac
     3301 gagcgtcgtt accgaatttt acgtctttag ctgccattat ctttattcct taaattcgta
     3361 tgttcagtgt cgtgcgcgga ttacgcttca acaattgcca gaatgtcgct ttcggacatg
     3421 atcaacactt cttcattgtc gatcttctcg gatttcacac cgtagccatc gttgaaaata
     3481 acgatgtcgc caactttcac gtccagcggc ttcacttcgc cattttcaag gatacggcca
     3541 ttgccgacag ccagcacttc gccgcgggtg gatttagccg ctgcagagcc ggtcagaacg
     3601 atgccgccag cagatttagt ttcaacttct ttacgcttga cgatcacgcg atcatgcaat
     3661 ggacgaatat tcattgataa ctctcctttg agaaagtccg tatctgttat gggtgacgcc
     3721 ggagcttacg tggtttcccg gctggtgacc agagaaatgg ggatgaggct tcgccccttc
     3781 aagggggaaa aagaaaaaaa attctgatca gcacaaaatc gggtgaaaac cctgattcac
     3841 ctcacatttc atcgcaattt cttcatcgcc gtcataagcg aatctgattg tgctaccatc
     3901 gaaaatctac gcagttgccc aaaatttggg cgcaatcgga tatcaaccca tgagtggact
     3961 caaacaagaa ctggggctgg cccagggcat tggcctgcta tcgacgtcat tattaggcac
     4021 tggcgtgttt gccgttcctg cgttagctgc actggtagca ggcaataaca gcctgtgggc
     4081 gtggcccgtt ttgattatct tagtgttccc gattgcgatt gtgtttgcga ttctgggtcg
     4141 ccactatccc agcgcaggcg gcgtcgcgca cttcgtcggt atggcgtttg gttcgcggct
     4201 tgagcgagtc accggctggc tgtttttatc ggtcattccc gtgggtttgc ctgccgcact
     4261 acaaattgcc gccggattcg gccaggcaat gtttggctgg catagctggc aactgttgtt
     4321 ggcagaactc ggtacgctgg cgttggtgtg gtatatcggt actctcggtg ccagttccag
     4381 tgctaatcta caaaccgtta ttgccggact tatcgtcgca ctgattgtcg ctatctggtg
     4441 ggcgggcgat atcaaacctg cgagtatccc cttccccgcg ccaggaaata tcgaacttac
     4501 cgggttattt gctgcgttat cagtgatgtt ctggtgtttt gtcggtctgg aagcatttgc
     4561 ccatcttgcc tcggaattta aaaatccaga gcgtgatttt cctcgtgctt tgatgattgg
     4621 tctgctgctg gcaggattag tctactgggg ctgtacggta gtcgtcttac acttcgacgc
     4681 ctatggtgaa caaatggcgg cggcagcatc gcttccaaaa attgtagtgc agttgttcgg
     4741 tgtaggagcg ttatggattg cctgcgtaat tggctatctg gcctgctttg ccagtcttaa
     4801 catttatata cagagcttcg cccgcctggt ctggtcgcag gcgcaacata atcctgacca
     4861 ctacctggca cgcctctctt ctcgccatat cccgaataat gccctcaatg cggtgcttgg
     4921 ctgctgcgtg gtgagcacgt tggtaattca tgccttagag atcaatctgg aggctcttat
     4981 tatttatgcc aatggcatct ttattatgat ttatctgtta tgcatgctgg caggctgtaa
     5041 attattgcaa ggtcgttatc gactactggc ggtggttggc gggctgttat gcgttctgtt
     5101 actggcaatg gtcggctgga aaagtctcta tgcgctgatc atgctggtgg ggttatggct
     5161 gtttctgcca aaacgaaaaa caccggaaaa tggcataacc acataatccg gcgtttcgac
     5221 attaatcctg gcgatcgtct ttatgatcaa ggcggtcgcg gtcatcatcc tttcgctgat
     5281 actcaccatc aaaagtatta ccgccaccgg tcccggcgct aaaaccgccg cctggcatgc
     5341 gagaaaagcg caaatgtggc atcaacttca ctgtcagatg cttttgcacc ggcggcaata
     5401 aaagcagaag accgaggaag tcggtgaaaa agcccggtaa taaaagcagc aaaccggcaa
     5461 taatcagcga aacactttta atcatctccg ccgcagggtt ttcacccgcc gccatttttt
     5521 gctgcatcag cacgaaattc ttaaagccct ggttacgtac cagtgacata ccgataactg
     5581 acgtgaatat aaccagcacg agggtcagca atacccccaa tacatgggca acctgaataa
     5641 agattgaaat ctcaatatag acataaagga aaatggcaat aaaaggtaac cagcgcaaag
     5701 gtttctcctg taatagcagc cggttaaacc cggctacctg aatgggttgc gaatcgcgtt
     5761 cagcttatat tgtggtcatt agcaaaattt caagatgttt gcgcaactat ttttggtagt
     5821 aatcccaaag cggtgatcta tttcacaaat taataattaa ggggtaaaaa ccgacactta
     5881 aagtgatcca gattacggta gaaatcctca agcagcatat gatctcgggt attcggtcga
     5941 tgcaggggat aatcgtcggt cgaaaaacat tcgaaaccac atatattctg tgtgtttaaa
     6001 gcaaatcatt ggcagcttga aaaagaaggt tcacatgtca aacaacattc gtatcgaaga
     6061 agatctgttg ggtaccaggg aagttccagc tgatgcctac tatggtgttc acactctgag
     6121 agcgattgaa aacttctata tcagcaacaa caaaatcagt gatattcctg aatttgttcg
     6181 cggtatggta atggttaaaa aagccgcagc tatggcaaac aaagagctgc aaaccattcc
     6241 taaaagtgta gcgaatgcca tcattgccgc atgtgatgaa gtcctgaaca acggaaaatg
     6301 catggatcag ttcccggtag acgtctacca gggcggcgca ggtacttccg taaacatgaa
     6361 caccaacgaa gtgctggcca atatcggtct ggaactgatg ggtcaccaga aaggtgaata
     6421 tcagtacctg aacccgaacg accatgttaa caaatgtcag tccactaacg acgcctaccc
     6481 gaccggtttc cgtatcgcag tttactcttc cctgattaag ctggtagatg cgattaacca
     6541 actgcgtgaa ggctttgaac gtaaggctgt cgaattccag gacatcctga aaatgggtcg
     6601 tacccagctg caggacgcag taccgatgac cctcggtcag gaattccgcg ctttcagcat
     6661 cctgctgaaa gaagaagtga aaaacatcca acgtaccgct gaactgctgc tggaagttaa
     6721 ccttggcgca acagcaatcg gtactggtct gaacacgccg aaagagtact ctccgctggc
     6781 agtgaaaaaa ctggctgaag tcactggctt cccatgcgta ccggctgaag acctgatcga
     6841 agcgacctct gactgcggcg cttatgttat ggttcacggc gcgctgaaac gcctggctgt
     6901 gaagatgtcc aaaatctgta acgacctgcg cttgctctct tctggtccac gtgccggcct
     6961 gaacgagatc aacctgccgg aactgcaggc gggctcttcc atcatgccag ctaaagtaaa
     7021 cccggttgtt ccggaagtgg ttaaccaggt atgcttcaaa gtcatcggta acgacaccac
     7081 tgttaccatg gcagcagaag caggtcagct gcagttgaac gttatggaac cggtcattgg
     7141 ccaggccatg ttcgaatccg ttcacattct gaccaacgct tgctacaacc tgctggaaaa
     7201 atgcattaac ggcatcactg ctaacaaaga agtgtgcgaa ggttacgttt acaactctat
     7261 cggtatcgtt acttacctga acccgttcat cggtcaccac aacggtgaca tcgtgggtaa
     7321 aatctgtgcc gaaaccggta agagtgtacg tgaagtcgtt ctggaacgcg gtctgttgac
     7381 tgaagcggaa cttgacgata ttttctccgt acagaatctg atgcacccgg cttacaaagc
     7441 aaaacgctat actgatgaaa gcgaacagta atcgtacagg gtagtacaaa taaaaaaggc
     7501 acgttagatg acgtgccttt tttcttgtga gcagtaactt aaaaataaca atataatatc
     7561 aacttgttaa agaacaagga aggctaatat gctagttgta gaactcatca tagttttgct
     7621 ggcgatcttc ttgggcgcca gattgggggg aataggtatt ggttttgcag gcggattggg
     7681 ggtgctggtt cttgccgcta ttggcgttaa acccggtaac atcccgttcg atgtcatttc
     7741 cattatcatg gcggttatcg ccgctatttc tgccatgcag gttgctggcg gtctggacta
     7801 tctggttcat cagacagaaa agctgctgcg ccgtaacccg aaatacatca cgatcctcgc
     7861 accgatcgtg acctatttcc tgactatctt tgctggtact ggcaacatct ctctggcgac
     7921 actgccagtt atcgctgaag ttgcgaagga acaaggcgtt aaaccttgcc gtccgctctc
     7981 tactgcagtg gtatccgcgc agattgcgat caccgcatcg ccaatctcag cggcagtggt
     8041 ttacatgtct tccgtgatgg aaggtcatgg catcagctac ctccatctgc tctccgtggt
     8101 catcccgtcc accctgctgg cggttctggt gatgtccttc ctggtcacta tgctgttcaa
     8161 ctccaaactc tctgacgatc cgatttatcg caagcgtctg gaagagggct tggttgaact
     8221 gcgcggtgaa aagcagattg aaatcaaatc cggtgcaaaa acgtccgtct ggctgttcct
     8281 gctgggcgta gttggcgtgg ttatctatgc aatcatcaac agcccaagca tgggtctggt
     8341 tgaaaaaccg ctgatgaaca ccaccaacgc aatcctgatc atcatgctca gcgttgcaac
     8401 tctgaccacc gttatctgta aagtcgatac cgacaacatc ctcaactcca gcaccttcaa
     8461 agcaggtatg agcgcctgta tttgtatcct gggtgttgcg tggctgggcg atactttcgt
     8521 ttccaacaac atcgactgga tcaaagatac cgctggtgaa gtgattcagg gtcatccgtg
     8581 gctgctggcc gtcatcttct tctttgcttc tgctctgctg tactctcagg ctgcaaccgc
     8641 aaaagcactg atgccgatgg ctctggcact gaacgtttca ccgctgaccg ctgttgcttc
     8701 tttcgctgcg gtgtctggtc tgttcattct gccgacctac ccgacgctgg ttgctgcggt
     8761 acagatggat gacacgggta ctacccgtat cggtaaattc gtcttcaacc atccgttctt
     8821 catcccgggt actctgggtg ttgccctggc cgtttgcttc ggcttcgtgc tgggtagctt
     8881 catgctgtaa tgatcaatcg cggggcgttc acgccccgct ttctttcccg ccgactaaca
     8941 tccttccccc gtccgttgta tagtgacctc tctcttgcgg ttccatctgt tcttgcgagg
     9001 tgtttatgct tgatgaaaaa agttcgaata ccgcgtctgt cgtggtgcta tgtaccgcac
     9061 cggatgaagc gacagcccag gatttagccg ccaaagtgct ggcggaaaaa ctggcggcct
     9121 gcgcgacctt gatccccggc gctacctctc tctattactg ggaaggtaag ctggagcaag
     9181 aatacgaagt gcagatgatt ttaaaaacta ccgtatctca ccagcaggca ctgctggaat
     9241 gcctgaagtc tcatcatcca tatcaaaccc cggaacttct ggttttacct gttacacacg
     9301 gagacacaga ttacctctca tggctcaacg catctttacg ctgattctgc tactttgcag
     9361 cacttccgtt tttgccggat tattcgacgc gccgggacgt tcacaatttg tccccgcgga
     9421 tcaagccttt gcttttgatt ttcagcaaaa ccaacatgac cttaatctga cctggcagat
     9481 caaagacggt tactacctct accgtaaaca gatccgcatt acgccggaac acgcgaaaat
     9541 tgccgacgtg cagctgccgc aaggcgtctg gcatgaagat gagttttacg gcaaaagcga
     9601 gatttaccgc gatcggctga cgcttcccgt aactatcaac caggcgagtg cgggagcgac
     9661 gttaactgtc acctaccagg gctgtgctga tgccggtttc tgttatccgc cagaaaccaa
     9721 aaccgttccg ttaagcgaag tggtcgccaa caacgcagcg tcacagcctg tgtctgtttc
     9781 gcagcaagag cagcacaccg cgcaattgcc cttttccgcg ctctgggcgt tgttgatcgg
     9841 tattggtatc gcctttacgc catgcgtgct gccaatgtac ccactgattt ctggcatcgt
     9901 gctgggtggt aaacagcggc tctccactgc cagagcattg ttgctgacct ttatttatgt
     9961 gcaggggatg gcactgactt acacggcgct gggtctggta gttgccgccg cagggttaca
    10021 gttccaggcg gcgctacagc acccatacgt gctcattggc ctcgccatcg tctttacctt
    10081 gctggcgatg tcaatgtttg gcttgtttac tctacaactc ccctcttcgc tgcaaacacg
    10141 tctcacgttg atgagcaatc gccaacaggg cggctcacct ggcggtgtgt ttgttatggg
    10201 ggcgattgcc ggactgatct gttcaccatg caccaccgca ccgcttagcg cgattctgct
    10261 gtatatcgcc caaagcggga acatgtggct gggcggcggc acgctttatc tctatgcgtt
    10321 gggcatgggc ctgccgctga tgctaattac cgtctttggt aaccgcttgc tgccgaaaag
    10381 cggcccgtgg atggaacaag tcaaaaccgc gtttggtttt gtgatcctcg cactgccggt
    10441 cttcctgctg gagcgagtga ttggtgatgt atggggatta cgcttgtggt cggcgctggg
    10501 tgtcgcattc tttggctggg cctttatcac cagcctacag gctaaacgcg gctggatgcg
    10561 cgtggtgcaa ataatcctgc tggcagcggc attggttagc gtgcgcccac ttcaggattg
    10621 ggcatttggt gcgacgcata ccgcgcaaac tcagacgcat ctcaacttta cacaaatcaa
    10681 aacgatagat gagttaaatc aggcgctcgt tgaagccaaa ggcaaaccgg tgatgttaga
    10741 tctttatgcc gactggtgcg tcgcctgtaa agagtttgag aaatacacct tcagcgaccc
    10801 gcaggtgcaa aaagcgttag cagacacggt cttacttcag gccaacgtca cggccaacga
    10861 cgcacaagat gtggcgctgt taaagcatct taatgtcctt ggcctaccga caattctctt
    10921 ttttgacgga caaggccagg agcatccaca agcacgcgtc acgggcttta tggatgctga
    10981 aaccttcagc gcacatttgc gcgatcgcca accgtgaacg acacttgcag tgggaaagac
    11041 ggaggaaaat agcgtgcaac gtgaagatgt actgggagaa gccctgaaat tattagaatt
    11101 acaagggatt gccaacacca cgctggagat ggttgctgaa cgtgtggatt atccactgga
    11161 cgagctacgc cgcttctggc cagacaaaga ggcaatcctc tacgatgcgc tgcgttatct
    11221 tagccaacag atagatgtct ggcgtcgtca gctgatgctg gacgaaacgc aaaccgccga
    11281 acaaaagctg ctggcacgtt atcaggcgtt atcggagtgc gttaaaaaca accgctatcc
    11341 gggctgtcta tttatcgctg cctgtacgtt ttatcccgat cctggccacc ctattcatca
    11401 actggccgat cagcaaaaaa gcgcggccta cgatttcacc cacgaactgt taaccacgct
    11461 ggaagttgac gatccggcga tggtagcaaa gcagatggaa ctggtgctgg aaggctgttt
    11521 aagccgaatg ctggtgaatc gtagccaggc ggatgtcgac actgcacatc ggctggcgga
    11581 agatatcctg cgcttcgccc gctgccgcca gggtggtgca ttgacctgac agaaacacag
    11641 aaaagaagcg atttgccgca atcttaagca gttgaatcgc ttttactgaa attaggttga
    11701 cgagatgtgc agattacggt ttaatgcgcc ccgttgcccg gatagctcag tcggtagagc
    11761 aggggattga aaatccccgt gtccttggtt cgattccgag tccgggcacc aaattcatat
    11821 caacggaccc ccacggaggt ccgtttttcg tttcaggacg ccacgattta agcgttctgc
    11881 ctccaaatca attctaccga actcaaccag attctcccca catcaccagc aatttgcgag
    11941 catatcccaa tttgggaaaa tttgttcctg agctatagca ctaactgacg tgaaatgtcg
    12001 tgcggccccg tgatgctgtt gaacgtcaaa tgacgtcatc aggagcgtaa cgtatccata
    12061 aagcacaaca ccggtctgaa cgccgactga tgagattttc tgaatgagaa caaagagaaa
    12121 tgtgtcagtc cgcttgctca tgcaaagact aac
//
LOCUS       DAETPZ010000110        11931 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.110, whole genome shotgun sequence.
ACCESSION   DAETPZ010000110 DAETPZ010000000
VERSION     DAETPZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.110"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..2252)
                     /locus_tag="K0W59_003473"
                     /pseudo
     CDS             complement(1..2252)
                     /locus_tag="K0W59_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            complement(2276..2899)
                     /locus_tag="K0W59_003474"
     CDS             complement(2276..2899)
                     /locus_tag="K0W59_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HBI3024367.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2896..4584)
                     /locus_tag="K0W59_003475"
     CDS             complement(2896..4584)
                     /locus_tag="K0W59_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HBI3024368.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRLRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWCREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="K0W59_003476"
     CDS             complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="K0W59_003476"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HBI3024369.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7507..8229
                     /locus_tag="K0W59_003477"
     CDS             7507..8229
                     /locus_tag="K0W59_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HBI3024370.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8357..11931)
                     /gene="yfaL"
                     /locus_tag="K0W59_003478"
                     /pseudo
     CDS             complement(8357..11931)
                     /gene="yfaL"
                     /locus_tag="K0W59_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2766 a         3310 c         2942 g         2913 t
ORIGIN      
        1 cattcgacat tgaccagttc accaggctgg taatgggttt tgtccgtttt cacccggata
       61 tcaagctgag gaacggcaac tttgatcccg gcgttctgaa aactgtactg accattacgg
      121 gtatacagca ccgaaaaagt gatgttaggc gcaaaggaat tgctcactgg aacccgggct
      181 tcatacaggg tatcgtttaa acgttgtagc gttagccagt ttgccggatg cgaaagcagc
      241 gactgctgtt ccacgcgatc gcgttccagc gtcaataacg cttcatcaat tggctccggg
      301 aaagtaatca gcatcttcgc ggtttcgcct ggctggtaca gcgttttatc cgccacgata
      361 tctaccgtac cagtatgcgc cgtgctgccc ttaccgctga cggcatgact taacccagcg
      421 agaattaagc cgtctttatc gcgtaatgtc agattgtagt tgccaggttt agcgaaattg
      481 acggtaaagg atttgccgcc tgacggtagc tctccgctat ggctcgtgcg gtcttcgaga
      541 cgcaaccatt catacgtaac aggaacctgt tttgaagatt ccagcgcggc ataacggaac
      601 acaaccgact cgccgctatt actgtattgt gcggcggtac ttaaagagta atgcgccagg
      661 ccgcgttcaa tgaggatctc tttggtggtg gtgacgcgat acgccgcgcc gtcactggcg
      721 gagacggtta acaaatagcg gctcggttta tcggcggcgg ggagatttaa cgccacatga
      781 ccgctggcgt cggacaccgt ttcgctgcct tccagcgaca cggggaaacg tccggcataa
      841 cgcaaatcgt taccgaccat tgataattgc tgagcgcgca aacttaactg cacgcgggca
      901 ttttttaccg gctcgccatc cgggtagagc agttgcagtt tgccgctgac cgcttcgcca
      961 gttttgaact cttttttgtc gagagctaaa ccaatctcga aatgtggctt gatgtagttt
     1021 gccacgcgaa aactgctgct atagacctga ttgcggtaag caagacgtaa ctcataacct
     1081 ccggcaacgg cattttctgg caggcggaaa cttccctgcc cgccattgcg cgcatccagc
     1141 gtgacatcga cggtttgcaa cagactgccg ttagcgtcca gcaccgaaag cttcgccggg
     1201 gcgctgacga tgggggatga atgcaacgga tcgcggaact cgcggcccat cactttaaca
     1261 tcgacacgat cgcctgcgcg atatagcggg cgatcggtaa aaatatacaa gcgggtgttg
     1321 tagatttcgc tttcgtagaa gaagttctcg gagacaaaaa cgccgccttc agcatcctta
     1381 cccagaatgt atgaacgttc tggcgatata tgttgtaact gcaaagtacc gctgtcatcg
     1441 gtcaccccgc gggtcatcac gccaagaccg tcagtccaca agatctctga gccgggcttc
     1501 gcttcaccct gttttttacc cgcggtccat accagaagct cgttacctga cactttgcta
     1561 agcgccacgg tatcggaaac aaacaccacc gtcgtcgccc gatacccacc taccatcgcc
     1621 tcgacgaggt acagccccgg ctcttgttgg ccgagaggaa tataaatatt acccggctgc
     1681 ggcgagatga aattgctgga tgcgccttcc agttttatcc cttgctgcgg ctcgaccggt
     1741 ttagcctgcc atagtggata acggaactgt tccaccaagg gatatttttt cagcggggaa
     1801 aactggttgt tctgtacata acgggaaggt ttaataatgg cattgccgag atgtaattcg
     1861 ggtaatgcct gagtcacatt ctgccgtgac tgagaagaga aagtacgctg catcacgcgg
     1921 cgagatttgc cgtaccagtt atcccacagc taggtcagcg tattgttcag cccgtcgccc
     1981 agatattgcg gttgcaccac aatgcgatgc aggtttttct gctggcgcaa aaatgccatc
     2041 gggtcaggaa tacgatacag gcgaacgtcc acgccgccgt actcttccat ctgatagcgc
     2101 cgataatcac gccccggcgc ttccagtcgc actttcgcct cttcactgct gctaaaactg
     2161 ctgtcagcaa gcaaaaagaa tgttcccccg gcgggcggcg catagttact ggaaggaagc
     2221 gaatcatcag ctttagcaag ccctgttccc acaagagaca gggacaagca cgctgctatc
     2281 gcgccagaaa atttaaacga taaatgccaa tgaaattggg attggaatcg ttgggtatcc
     2341 atcgggtgtc cttccatgtc ataagttgtt gcagactgac tgcgcgcatt ccgttgtcag
     2401 ttttcgtggc gcttccggtg tggtagatga cgtaacgccc catccagacc attaagtgct
     2461 gggcatcgcc ctgatcgaaa aaaatcatat cgccaggcag cgcctggttt atgtcttggc
     2521 caataaactg gctgttgtac tgaatcaaat taatcgcggt cacgtagggg ccgtttttcc
     2581 cgctcccctg attccagttt tgcgccagtt gacgctgttc aggtgttagc gtcatctctg
     2641 gcggcaaata ctggctagat aaaccgttac tttttagcca tttactgtcg tgaactttca
     2701 gcgtctcgtt cgccgcaaat cgcaccaggc ccgcacaatc ctgctgatac cagcgtggac
     2761 ttggcccctg gcggagctgt tcttgtgcaa tgcgtacaaa ccaggcgcga aacagcctgg
     2821 attgttcgac attcagcatt tcgctatggg caacaacaca acacagccag caaatcagcg
     2881 ccagcagccc gtgtctcata gcggctgcca ggttatgggt agccactgcc aggcgacacc
     2941 gggttccatc tgggccagct tcatgacata acgcggttgt tgagataaag cgtccagctt
     3001 cggcattaat aaagtttgtg cggcgttata aaaaaccggt tcgagattct tcggcagact
     3061 ggtcaacgtt tcgttacgca gcagtttcgc cacgccttgt ggattgatat agagcggaac
     3121 gatgccatca gtgggtatta catccaccat tgccgggcgg gttttattca gtgtttgcag
     3181 ggcgttatta accagcgtgt catcgaggga gaaaagcagc gttttgtttt gcatcgccag
     3241 cgacacacgg aaaaaataat ccgacattaa ttgatcgggt tgcgccgcct gcgctttcgg
     3301 atactgaccg tatcgggaac tcacttcgcg acaccagatt tgcgcttcgc cctgctgagt
     3361 ctggcttacc ggcaaaacac cttctggcgc tttgctttcg tgcgcaccaa tattttgcgt
     3421 aaacagtttc cctggcaatt gcgcctgttc ggcagtgcca tcaaactgac cgacaaacag
     3481 cggggtttgc aattttgagt cttcatacca gcacagcccc gcggcaccgt ctaacgcccc
     3541 attcagcttg tcgttttcct ggctgatgtg cgaaagcatc tcttcggcaa taccgtgtga
     3601 atacggcacc gccacacaga agctggctcc ggcaggcata ctgttccaga ccggcgtaaa
     3661 atcgaaactg gcatctacgc tggcggattc atcatttaac gccacaaaac tgtgccagcc
     3721 gtcgttaccc atttcgaagc gtacgccagc aaaagaaggc attaaccgct ggtagccaaa
     3781 acccagcaac ctggcgctgg ccacgatgcg caggcgtact ggcgtttttt cggctgtacg
     3841 ctcttccagg ccaaagctgg cttgccagcg ttttttaccg ctcaacaaat cacttgcgat
     3901 cgccgtggct tcggtatcct gctgatcatc tttaaacaac atatccgtac tggaaaacac
     3961 cagcatcttg tcctgatatg tcgcgaacat cagggcgtta ttgccgttat agcgcaactg
     4021 ataaacagga atagtttcac tatttatctt gatgctactg atttccgttt tgcttaactg
     4081 gctgtcgctg gtagcggcaa acaacaatgg ctccagcagt ttgcttaaac cactgcgctg
     4141 gatcagcacc atgtaatgtg aaagatggcc ttgtttatcg tgccatagcg ccgcctgcgc
     4201 gggctgatct aagagtgacg aaaagagctt atctttcagc gtgagatcgt gttcatagac
     4261 aatgcggcga atgctgcctt caatgcccag acgatcggca tgattctgat aatagaaaac
     4321 gaaatcctcg ctcagaacat cgtggagaaa cggaatggtg aggagatctt tgggaagctg
     4381 gctcagagag tcgctgtcta gaaagaggtc cggctcattg agatcgattt gcagattgtt
     4441 gtgcaccacc agcggcgaca acgttttttc tggcccactg ccagcatatt gcaacgccca
     4501 gacgccagcg gaaagcagtg ctattgcgcc aaaacctaca agaccataga accgccagcc
     4561 tttcgccttt ttttcaccac tcattgccac attccttgtg tatagccagc cattttttac
     4621 gggcacagcc aaactttacc gcgccctaat acgacaaaag cccagacttt gcagcctgga
     4681 cttttcaatt caaacaaggg agatagctcc cttttggcat gaagaagtaa aattattctt
     4741 cttctggctc gtcgtcaacg tccacttccg gagcaatttc atcatcccct tccgcggcac
     4801 tgccgtcgat ggtatccaga tcttcctcgt caaccggttc agcaacacgc tgcagaccca
     4861 ctacgttttc atcttccgca gtacggatga ggatcacgcc ctgggtgtta cggcccacga
     4921 tgctaatttc tgaaacgcga gtacgtacca gcgtaccggc atcggtgatc atcatgatct
     4981 ggtcgcagtc atctacctgc accgcgccaa ccactaaacc gttacgttcg gtaaccttga
     5041 tggagataac ccctttcgtc gcacgcgact tggttgggta ttccgccact gcagtacgtt
     5101 taccgtaacc gttttgcgtt gcggtgagga ttgcgccatc gccacgaggc acgatcagag
     5161 agacgacttt atcgccttca cctaagcgaa taccgcgcac accagtggta ttgcagccca
     5221 tcgcacggac agaagactct ttaaagcgca ccactttacc ttcggcggag aacagcatta
     5281 cttcatcttc gccactggtc agatcaacgc cgatcagctc atcgccgtca accagtttga
     5341 tcgccacttt accggcggta cgcagacggt tgaattcggt gaggacggtt ttcttcacag
     5401 taccgttagc ggtcgccatg aagactttca cgccttcttc aaactcggtc accggcagga
     5461 tcgcggtgat acgttcgtcc tgttccagcg gtagcaggtt gacgatcgga cgaccgcgcg
     5521 cgccacgagt ggcttccggc aactggtaaa ctttcatcga atagacgcga ccacggctgg
     5581 agaagcacag aatatggtcg tgcgtgttcg ccaccagcag tcggtcgata aagtcttctt
     5641 ctttaatacg tgcggcagat ttacctttcc cgccacgacg ctgcgcttcg tattcagaaa
     5701 gcggctgata tttaacgtag ccctggtgag agagcgtcac gaccacatct tcctgggtga
     5761 tcagatcttc caggttgatg tctgcgctgt tggcggtgat ttcagtacga cgtttgtcac
     5821 cgaactgttc acgaaccagc tccagctctt cgcggatcac ttccatcaga cgatcggcgc
     5881 taccaagaat acgcaacagt tccgcgatct gatccagcag ctctttgtat tcgtcgagca
     5941 gtttttcgtg ctcaaggccg gtcagtttct gcaaacgcag atccagaatc gcctgagctt
     6001 gctgttcggt caggtagtac agaccatcac gcacgccgaa ctctggctcc agccattccg
     6061 gacgcgcagc atcgtcgcca gcacgttcca acatggcggc aacgttgccc agctgccacg
     6121 gattagcaac cagcgcagtt ttcgcttctg caggcgtcgg cgcatgacgg atcagttcga
     6181 tgatcgggtc aatgttcgcc agcgccacgg ctaatgcttc aaggatatga gcacgatcgc
     6241 gagctttacg cagttcgaaa atagtacgac gggtcaccac ttcacggcgg tgacgaacaa
     6301 acgccgcgat gatgtctttc aggttcatga tcttcggctg accatggtgc aatgccacca
     6361 tgttgatacc gaaagaaacc tgcaactggg tctgggagta gaggttgttg agcacaactt
     6421 caccgaccgc atcgcgtttc acttcaatca cgatgcgcat accgtctttg tcagactcgt
     6481 cacgcagcgc gctgatgcct tccacgcgtt tttcttttac cagttccgca atcttctcga
     6541 tcaggcgcgc tttgtttacc tgatacggaa tttcgtggac gataatggtt tcacgtccgg
     6601 ttttggcgtc aacttccact tcagcacggg cgcggatata taccttgccg cgaccggtac
     6661 ggtaagcttc ttcaataccg cgacgaccgt taatgattgc cgccgtcggg aagtccggcc
     6721 ccgggatgtg ttccatcagc ccttcaatgc tgatgtcttc atcatcgata tacgccagac
     6781 aaccgttgat gacttccgtc aggttgtgcg gcgggatgtt ggttgccata cctacggcga
     6841 taccggaaga accgttcacc agcaggttag gaattttggt tggcatgacg tcgggaattt
     6901 tttccgtgcc gtcatagtta tcaacgaaat cgaccgtctc tttttcgaga tcggccatca
     6961 gttcatgggc aattttcgcc agacggattt ccgtataacg cattgccgcc gcagagtcgc
     7021 cgtcgatgga accgaagtta ccctgaccgt ctaccagcat gtaacgcagc gagaatggct
     7081 gcgccatacg gacgatcgtg tcataaaccg ccgagtcacc atggggatgg tatttaccga
     7141 ttacgtcacc aacgacacgg gcagattttt tataggcttt gttccagtca ttgcctagta
     7201 cgttcatggc gtaaagtacg cgacggtgta ccggcttcag gccatctcgg acatctggca
     7261 gcgcacggcc aacaatgacc gacatcgcat aatccagata ggagctcttt agctcttcct
     7321 caatgttgac cggtgtaatt tctctcgcaa ggtcgctcat ctaaccgcta tccctctact
     7381 gtatcccgga ttcaaaggtc gcaaattata acacagccgc gcagtttgag gtaaacctat
     7441 acgctttatt cacatccaat gcctgatata ctcgtttgtc ttgccaatta cggagtagaa
     7501 gtgccaatga atgccgaaaa atcgccggaa aaccataacg tagaccacga agagatcgct
     7561 aaatttgaag ccgtcgcctc ccgctggtgg gatcttgaag gtgagttcaa accgttgcac
     7621 cgcattaacc cgctgcgtct gggctatatt gccgagcgtg ctggcggttt atttggcaaa
     7681 aaggtgctcg atgtcggttg tggcggcggc attctggccg agagtatggc gcgcgaaggc
     7741 gcgacggtga ccggtctgga tatgggcttt gagccattgc aggtggcaaa gctgcacgcg
     7801 ctggaaagcg gcattcaggt ggattacgtg caggaaaccg tggaagagca cgcggcaaaa
     7861 catgccgggc agtatgatgt ggtgacctgc atggagatgc tggagcacgt acccgatccg
     7921 cagtcagtgg tcagagcctg tgcgcaactg gtgaaaccag gcggcgatgt ctttttctcg
     7981 acgcttaacc gcaacggcaa gtcatggctg atggcggtgg ttggcgcgga atatattttg
     8041 cgcatggtgc ccaaaggtac gcatgatgtg aagaagttta ttaaaccggc agagctgctt
     8101 ggctgggtgg atcagaccag tttaaaagag cgacatataa ccgggctaca ttacaacccg
     8161 atcactaatt cctttaagct cggccccggc gtggatgtga actatatgct gcacacgcag
     8221 aataagtgag gttgatgttt ggccgcgcca atgcctgatg cgacgcttgc agcgtcttat
     8281 caggcctaca aatgctctcc gtaggccaga taaggcgttt acgccgcatc cggcaattgt
     8341 gatggtcaga cagcgattac catttcaccg tcatcgacaa aaaccctgcc gtctgggcaa
     8401 aatcatcact ccctttctgc cacgccacgc tgccgcgcag agacactcgc tgactgatat
     8461 tgcccgtgac tcccactttt atttcacccc gtagcttcac cgcatcgtca ctgatagtgc
     8521 tgccatcttc ttcaatttcc gtcgcatggg gatcgtgata ataattcaga tctaatgttg
     8581 gtgtgacacc aaccgcggta cgccattcgc tgtgtaaacc cagccgcgtc tgaatatcat
     8641 ccccctgcga ttgtgacacg cgcgcatggt tagcggcggt aaaatcatcc tgctgcacgc
     8701 cctgataaat cacctgcgcc tgcggttcaa tcaccacacc acgccccggt aaccactgat
     8761 acccggcttc cagcgaggcg ataatccccg acgaatggta acgatccacg ccatcttcat
     8821 gttcggaaac atcattgtta aaccacgcgt attgcagcca gctatccagc caggccccct
     8881 gcttctgatt accgtgctga taccagcttg aggtcagccc aacggcataa ccgtggttct
     8941 ggttatcggc gcgagttccg gtcatattcg agcggctgtc gccctgatta tcactgtagc
     9001 caccaaccgc cccaagcatc cactcgccat ccgtgcccca gcacccgcta aacaagccgc
     9061 cgctaagcag caccgtagaa gtgtcttcct gctgagccag ttgccctgcc gccgtgtaat
     9121 gataacgtcc gccgataaca cgtaaattca gcgtctgacc atcgccacca gcgtgatcgc
     9181 gtcgctccat cataaacgcc tgatttgccg cccgcaggtt attgagataa ccgccaactt
     9241 tggcattcag caccggctgg taagcaggcg taggggccgg gtcgggggtt gggtcaggat
     9301 ccggcgtggg atcaggatcg ggagtcgggt ctggctcagg tggcgatggc ggcgttactt
     9361 cttgcgatcg cagataccag tcgttgttat cttccactag cgtgtagtca tacgctccca
     9421 tgttgacgta gccactgcca gccagactga actgtgcatt gttttgaaac tgcgtgggat
     9481 cagctgcgaa atcaaccact ttaatgcctg tcgatgtcgg ctcaccaatc cctgtaatgg
     9541 agttaaccac cacagttgtg ttgccagcag tattaccgtt catcaccaat tgatcgctta
     9601 ccgagtcatc gccgtttaat tcgctgtcga atagtagcgt accgccgccg gtataatcac
     9661 cgtttaccgt cagcgtatcg ccagcgacgc cattttgcag gctaaccgca ccggacgtat
     9721 tggtcagatc accattaacc gtcacgtcag caagataggt caggctgtca gtttcaaggt
     9781 tggtattaag ctcgcctgtc aacgtcacgc catcggtagc atttaccagc gtcccatcgc
     9841 cgttaaatag tgaggcatta agggcgacgg aagtatcctg cccggtcaga cgcaaaaccg
     9901 taccatcgct gatgtcgata gtgccgctgg aagtagcatc aattgactga atatcctgat
     9961 ccgcgccagt aacgaacgtt gccccatcac caacccacag tgacgaagca taaggaaaga
    10021 tatccgcaac atcgcctttc agggtgcctt cttcgacacg taccgccgac tgcgttgtac
    10081 cgctggcggt cagctccagc gtacccgccc ccgttttagt aaatgtgcta ccctcatcct
    10141 gatgctgccc gctgctgtca gccatcagtg cgccccactg cgtgtctacc cccgcatcaa
    10201 ctgccacttc accgtcggcg cgcatttcaa tatcacgccc atgctcagtc gtcgtagagc
    10261 gagtatcgac attggcagta atctccatca cgctttcttg ttacttatcg gtaaagataa
    10321 cctggcggtt atagtgggta tctccaagct gcgagttgtc gctcaccatc agcgtaccgg
    10381 aggcgatttg tgttgtgccc aaataactat tattgtttgc cagacttacc tgcccgtcgc
    10441 tcgcatcgac caccacgtag acgccatctt tcccgtcacc gccgatatgc aaattgtcgc
    10501 cacctgcaag atctaccaca tcctgactgc cgataaccgt tccgctgctg ccactgactt
    10561 caaggccatc attgtatgat gtgccgctct gccaagtgga gaaatcagag agatcgagca
    10621 caccgccgtt taacacgatc gactgcggat cgtcctggag agcggtaaga tctgccgcgt
    10681 cgccttccag cgaaagcacc gcaccatcat caacgactat atcgccggtt agcgccatcg
    10741 actgcgcccc tgccagcacg tagctgccgt tttgcgcaat ggtgagctga ccagcacctt
    10801 cgatgatgcc agcaaaactg ccctgattaa cagtaacgtt gccaccagca tcgatattta
    10861 aagtgccatt ctgaaagccc gtcaatgcgt gcacaaaagt ttgttgggtc gagccaacgt
    10921 ttagctcagc ctgattctga tactgatcaa tactccctat cgtcagaccg tagcagtctt
    10981 gcggatcgtc ctggcaatgc gtatcgccga cattcatcag ggagttgctg cgacccaggg
    11041 taacttcacc gttttcaatc tgcatctcac cagtaaagtc attgttatct gcattaagta
    11101 ccagatcgcc ggaacctgtt ttggtgatta acccggtacc agcaatagag tcaacagctc
    11161 cgtcattctc tgtattgccg ataaccagcg tttttccgtc ggcaatatca aaggtaactt
    11221 cacttaagcc gagatacata aagccacccg ccgcagtgga aggaccatct ccatagcctg
    11281 ctgcgctatt gttctcatcg actaacacgc cgccgttctg gctgtagctg tcatcaacag
    11341 aaatatcaat aagataggga gccctcacgc tattggtata tatcgccccg ccataacctt
    11401 cggctgtgtt atttgtaaag gcggtgttat ttattatcgt ataacctgaa ggatgcttgc
    11461 tgtcgctatt attatcggta acatcgattg ccccgccatc gccatcactg taacttgttg
    11521 atgtatatgc ctggttattg ttaaaaacat catcacttaa atagacgtca ttattaatgg
    11581 tataaattgc gccacctttg ccatcattag cgatattatt gcgaaacatg gcgttagtga
    11641 cacgtaaatc gacggcacca gtatcattag taccagaaga atagattgcg ccactatagc
    11701 cgcctgcgac gttaccggaa aaaataacat cagtaagatt cagcgttgag ttttctttag
    11761 caaatatggc cccgccgtta ttatattctc ctgtaacggt gttattggca aacagagtca
    11821 ttccagtttc attttctggt aatagtgaaa attccgctcc ttgttgcaaa aatacggccc
    11881 caccgctggc attattggtc atatccgaaa aaaccagcca ttgcccatcg g
//
LOCUS       DAETPZ010000111        11788 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.111, whole genome shotgun sequence.
ACCESSION   DAETPZ010000111 DAETPZ010000000
VERSION     DAETPZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.111"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            202..537
                     /locus_tag="K0W59_003479"
     CDS             202..537
                     /locus_tag="K0W59_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HBI3024371.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            630..713
                     /locus_tag="K0W59_003480"
     CDS             630..713
                     /locus_tag="K0W59_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024372.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            813..1634
                     /locus_tag="K0W59_003481"
     CDS             813..1634
                     /locus_tag="K0W59_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HBI3024373.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1673..2002)
                     /gene="mdtI"
                     /locus_tag="K0W59_003482"
     CDS             complement(1673..2002)
                     /gene="mdtI"
                     /locus_tag="K0W59_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HBI3024374.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1989..2354)
                     /gene="mdtJ"
                     /locus_tag="K0W59_003483"
     CDS             complement(1989..2354)
                     /gene="mdtJ"
                     /locus_tag="K0W59_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HBI3024375.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2765..3799
                     /gene="tqsA"
                     /locus_tag="K0W59_003484"
     CDS             2765..3799
                     /gene="tqsA"
                     /locus_tag="K0W59_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HBI3024376.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMEMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVHFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3824..5212)
                     /gene="pntB"
                     /locus_tag="K0W59_003485"
     CDS             complement(3824..5212)
                     /gene="pntB"
                     /locus_tag="K0W59_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HBI3024377.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARSINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5223..6755)
                     /gene="pntA"
                     /locus_tag="K0W59_003486"
     CDS             complement(5223..6755)
                     /gene="pntA"
                     /locus_tag="K0W59_003486"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HBI3024378.1"
                     /translation="MQIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFNE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7279..8223
                     /locus_tag="K0W59_003487"
     CDS             7279..8223
                     /locus_tag="K0W59_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBI3024379.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            8409..9791
                     /locus_tag="K0W59_003488"
     CDS             8409..9791
                     /locus_tag="K0W59_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI3024380.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQDAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9828..10550
                     /gene="folM"
                     /locus_tag="K0W59_003489"
     CDS             9828..10550
                     /gene="folM"
                     /locus_tag="K0W59_003489"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HBI3024381.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10547..10882)
                     /locus_tag="K0W59_003490"
     CDS             complement(10547..10882)
                     /locus_tag="K0W59_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HBI3024382.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKKKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            11011..11754
                     /gene="rstA"
                     /locus_tag="K0W59_003491"
     CDS             11011..11754
                     /gene="rstA"
                     /locus_tag="K0W59_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HBI3024383.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
BASE COUNT         2996 a         3011 c         2835 g         2946 t
ORIGIN      
        1 gctttgtaag tagtataata gttaacccga tcaagactac tattattggt agctaaattt
       61 tccttaagtc acaatacgtt attatcaacg ctgtaattta ttcagcgttt gtacatatcg
      121 ttacacgctg aaaccaacca ctcacggaag tctgccattc ccagggatat agttatttca
      181 acggccccgc agtggggtta aatgaaaaaa caaattgagg gtatgacaat gaaaaaagta
      241 ttagctctgg ttgttgccgc tgctatgggt ctgtcttctg ccgcctttgc tgcagagact
      301 gcgaccacac ctgctccgac tgcgacgacc accaaagcag cgccagcgaa aactacacat
      361 cataaaaaac agcataaagc agcacctgcc cagaaagcgc aggcggctaa aaagcatcat
      421 aaaaatacga aagctgaaca gaaagcccct gaacaaaaag cgcaggcagc gaagaaacac
      481 gccgggaaac acggccatca gcaaccggca aaacctgctg cacaacccgc agcgtaagtt
      541 ttcaacagta atgctggcgc gccccctcgc gcctgaaaat tacggtgcta agcgggtaac
      601 gtttagcacc gcctttagcg gagggtagta tggtgggccg ttatcgcttt gagttcattc
      661 ttatcatcct tattttatgc gcactgattg ccgcccgttt ttatctttcc tgactgtagt
      721 tatctgattt tactcccact ttcatcccgt cccgtctata gtatttatga gggtttgctt
      781 ttaataatca taattaccca ccagagtgtg atatgcgtac aaccattgct gtagtgttgg
      841 gtgcaattag tttgacgtct gcttttgtgt ttgcagataa accagacgtt gccaaatcgg
      901 caaacgatga ggtcagcacc ctgttttttg gtcatgatga tcgtgtgcca gtgaatgaca
      961 cgacccaatc accgtgggat gcggttgggc aactggaaac ggccagcggc aatttatgta
     1021 cggcgacgct gattgcaccc aatctggcat taacggcagg acactgttta ttgacgccgc
     1081 ctaagggtaa agcggataag gcggtagcgc tgcgttttgt gtcaaataaa ggtctttggc
     1141 gctatgagat ccacgacata gaaggccgcg ttgatccgac actgggaaag cggttaaaag
     1201 cagatgggga tggttggatt gttcctcccg cagctgcacc gtgggacttc ggattgattg
     1261 tgctacgtaa tcccccttct ggcattacac cgttgccgtt atttgaggga gataaagccg
     1321 cgcttactgc cgcattaaaa tcggctggtc gtaaagtgac tcaggcggga taccctgaag
     1381 atcatctcga tacgttatac agtcatcaaa actgtgaagt gactggctgg gcgcaaacgt
     1441 cggtgatgtc acatcagtgc gataccttgc cgggtgacag cggttcgcct ctgatgttgc
     1501 ataccgatga cggctggcaa ttaattgggg tgcaaagttc ggctcctgcc gcgaaagatc
     1561 gctggcgcgc cgataaccgg gccatttctg ttaccggttt tcgcgacaag ctggatcaac
     1621 tgtcgcaaaa ataatgttca aacgctgccc gacagcgcgg gcagcgtctt catcaggcaa
     1681 gtttcaccat gatcattcca gccaacagca agaccaggcc aatccagcct ttacgattta
     1741 accgttgacc aaacaagatc caacctgcgg ctaacgtggc ggcaatacca aacccgcccc
     1801 acaatgcata agcgacagac aagtcgatcc ctttaacggc ctgagaaagc gcactaaagg
     1861 ccgccagcac cgccgccagg gagagcaagc caaatatttt gcgacgaaag ccgtcagaaa
     1921 atttcaaaaa gacgttagca acgatttcca gcacgattgc caatgccagc caggcggcgt
     1981 gaacccattc aaactgcgcc atggttcacc tccagttcag gtttacgcgc tttacgggta
     2041 cctgatttga tcaacactat cccggcgacc agggtggtta acccggcaat tttcatcagc
     2101 gataaacttt cgtcgaataa caaaacgcta aacaaggtaa taaataaaat accgatacct
     2161 tcccacagcg cataagctac gcctaaggcg atttttttaa cggcgaaaga gagaaatata
     2221 tacgacagag aaatcatcac cagcattaaa ataaagccgc catttccctc actgacgctc
     2281 gcccatttca ttgacagcgt accggtaatt tctgtagcaa tagccagacc taataaaatc
     2341 caataaatat acattgtcct tctcctgcaa gagaactatt ttaattttcg cttaattcag
     2401 cgaaaccaaa gtaaagtggt caagccctgc gcatagggca agcttaggca gaagaaagga
     2461 ctaaagcgcg ttgcgccagt gctgctcacc tacgagcaag atagtggatg aggtacgaag
     2521 atgggtaaat gtagaaaata acgtcctgaa caaattgtcc ataatattac aattatccgc
     2581 agtgttgctt ctcgtcatcg cagatgataa ttgtcctcgg tagttgaaca cgcctgattt
     2641 gtatcatagc ttaagaatta actcaaaata ttttcacttc tttacctgag cggtttgatt
     2701 ttcgttatga tgacggagcg aaaaagacat tattattagc aaaggaagaa aaacggggac
     2761 aagcatggca aagccgatca tcacgctcaa tggcctaaaa atcgtcatta tgttgggaat
     2821 gctggtcatt attctctgcg gtatccgttt tgccgccgag atcatcgtgc cgtttattct
     2881 cgcattattt attgctgtta ttcttaaccc gctggtgcaa catatggtcc gctggcgtgt
     2941 gccgcgtgta ctggcagtgt cgattttgat gacaatcatc gtgatggaga tggtgttgct
     3001 attagcttat ctgggttccg cgctcaacga gttgacgcgg acgttaccgc aatatcgcaa
     3061 ctctattatg acgccgctgc aagctcttga gccgttgttg caacgcgtag ggattgacgt
     3121 ctcagttgac caactggcgc attacattga tccgaacgcg gcgatgacgt tgctcaccaa
     3181 cttattgacg cagttatcta atgccatgtc atcaatattt ttattgctgc tgacggtgct
     3241 gtttatgctg ctcgaagtgc cacaattgcc cggaaaattt cagcaaatga tggcgcgtcc
     3301 ggttgaaggg atggcggcga ttcaacgtgc gattgacagt gtttctcatt atctggtgct
     3361 gaaaacagcc atcagcatca tcaccggctt ggtcgcctgg gcgatgctcg ccgcactcga
     3421 tgttcacttc gcttttgtct ggggattgct ggcctttgcg cttaattaca tccctaatat
     3481 tggttcagtt ctcgcggcaa tcccccctat cgctcaggta ctggtgttta atggcttcta
     3541 cgaagcgttg ctggtgctgg cgggatatct gctgattaat ctggtcttcg gcaatattct
     3601 ggagccgcgc atcatgggac gtgggctggg gctttcgaca ttggtggtat ttttgtcgtt
     3661 gattttttgg ggatggttgt taggaccggt gggtatgctg ctttccgtgc cgttgacaat
     3721 tattgtcaaa attgcgcttg aacaaacagc gggaggtcaa agcatcgccg ttctgttaag
     3781 cgatctcaat aaagagtgac ggcctccgca gaggccgtca gggttacaga gctttcagga
     3841 ttgcatccac gctggctttg gcgtcaccaa acagcatgtg ggtgttttcc ttgaagaaca
     3901 gcgggttttg cacaccagca tagccagtgt tcatcgaacg tttaaagaca atcacgttct
     3961 gcgctttcca cacttccagc acaggcatac cagcaatcgg actcttcgga tcatcctgcg
     4021 ccgccgggtt aaccgtatcg ttagcaccaa tcaccagtac ggtatcggta tcagcaaagt
     4081 catcgttgat ctcgtccatt tccagcacga tgtcatacgg tacttttgct tcagccagca
     4141 atacgttcat atgtccaggc aaacgccccg caaccgggtg gataccgaaa cgcacattaa
     4201 tactacgagc gcggagtttc tcggtaattt cagcgacagg atattgcgcc tgcgcgactg
     4261 ccatgccgta ccccggagta atgatcactg aatgggagtt tttcagcagt tccgctgtct
     4321 cttctgcggt gatttcgcgg tgctcaccca cttcctgatc atcgccagta gaagagccgt
     4381 cggtgccgaa accacccgca ataacgctga taaaggaacg gttcatcgcc ttacacataa
     4441 tgtaagaaag gatagccccc gaagaaccga ccagcgcacc ggtcacaatc agcaggtcgt
     4501 tgctgagcat aaagcccgca gccgcagccg cccagccgga gtacgagttc agcatcgaca
     4561 ccaccaccgg catatctgca ccaccgatgg aggcgactaa atgccagccg aataccagcg
     4621 caatggcggt cattatcagc aatgccagca cttgcaggcc gacgctgtcc gtgcgaacaa
     4681 atacaatcag cagcaagaag gaaacgacca gagccgccag gttcattttg tgacggtttg
     4741 gcagcatcaa cggtttagac gaaatcttgc cacacagttt gccgaacgcc accaccgagc
     4801 ccgtgaacgt taccgccccg atgaagatac cgaggaacac ttccgtcagg tgaatattga
     4861 ccagaatcgg tgccattccc gcgtcatgat gcagataact gttaaagcca accagcactg
     4921 ccgccagacc cacgaagctg tgcaggatcg ccaccagttc tggcatttcg gtcatttcaa
     4981 ctttcttcgc cagccggata ccaattgccc caccaatgac catcgccagc aagatccagc
     5041 caacattacc cgtatccggt ccaaaaatgg ttgcgattaa cgcaatcgcc atcccggcga
     5101 taccgaagtt gttaccctgg cgagacgttt catgtttcga aagaccggcc agactgaaga
     5161 taaacaggat cgcggcaaca atgtatgcag ctgtaactaa tcctccagac atatgttacc
     5221 ccttaatttt tgcggaacat tttcagcatg cgctgagtca cggtgaagcc accgaaaata
     5281 ttaatgctgg ctataagcac cgcgataaaa ctaaggaagc taacccagcc tccctggcca
     5341 atctgcaaca gtgctccgac aacaataatc cctgaaatcg cgttggtaac cgacatcaac
     5401 ggtgtatgca gcgcgtgcga tacattccac accacgtaat aaccgacaac gcaggccagc
     5461 gcgaaaacgg tgaagtgccc aagaaactct ttcggcgcaa cgcttgacag ccaaccgaac
     5521 aggatgattg ccagcgccat caacgcgtat ttacgccacg gtgagcaggc acatttttcc
     5581 tcagttttca cttccggtgc cgctttttgt gccgcctgcg gctgagctga tacctgaatc
     5641 ggcggtgccg gccaggtaat ttcgcccgca cggatcacgg tcacgccgcg aatcaccaca
     5701 tcatcaaaat caacagtgat attgccgtct ttctctttgc acaacagttt cagcagatta
     5761 acgaggttag taccgtaaag ctgtgaggat tgcgtcggca gacggcccgg aagatcggta
     5821 taaccaatca ctttgacacc attttccgta ctgaagattt cacccggcac gatgtattca
     5881 cagttgccac cgttttgggc tgccaggtcg acaatcacac tgcccgcctt catggagtca
     5941 accatttcac gggtaattag cttcggcgct ggtttgcctg gaataagcgc ggtggtgaca
     6001 atgatatcga cctcttttgc ctgggcggca aagagttcca tttccgcttt gatgaacgcg
     6061 tccgacatca ctttggcata gccatcgccg ctgcccgctt cctcgttaaa atccagctcg
     6121 aggaattccg cgcccatact ttgaacttgt tctttcactt ccgggcgggt gtcgaatgca
     6181 cgcacaatcg cgccgagact gtttgctgcg ccaatggcgg ccaaacctgc aacaccggca
     6241 ccaatcacca tcacttttgc cggtggcact ttcccggccg cagtgatttg cccggtaaag
     6301 aagcgcccaa attcatgtgc cgcttcaaca atggcgcgat aaccggcgat attcgccatc
     6361 gagcttagtg cgtccagcga ttgtgcgcgt gagatacgcg gcacagagtc catcgccatc
     6421 acggtcacgt tacgttccgc aagtttttgc attaattccg gattctgcgc aggccagata
     6481 aaactcacca gcgttgtccc gggattcagt aacgcaattt catcatctaa cggcgcattg
     6541 accttcagaa tgatctctga ctgccagacg ctattccctt ctacaatttc agcgcccgct
     6601 tgcacaaacg ctttatcgtc aaaacttgcc agttgacccg cgccgctctc taccgcgacg
     6661 gtaaaaccca gtttcagcag ctgttccact gtttttggcg ttgctgcaac acgggtttca
     6721 ttggttaacc gttctcttgg tatgccaatt tgcatgatat tcccttccat cggttttatt
     6781 gatgatggtt tgcctgtgtc aggagccaca caagccgctc atgtacgagc taaatgttac
     6841 tccgttaaaa taaattagta acaaacgccc tataacgtac tgaaaattat gcctgtgatc
     6901 tagcgccaaa aatcagtatt tcggcgtgaa ctcgcaaaat attaacgatt cagccgtgat
     6961 agtgggataa acaccttaga acgccggata aagactgata attgtcttcg acggtcgggt
     7021 aaaacgagac atcgccccgg cacgaatcac tacttaacat taaattaact tatacaattc
     7081 agttgcttca gtagtaatga tgctgatacg gctgtttttt aagcatagac ggtcatttga
     7141 gcaggattaa aattggttta aggaatgtga tatgaaaaat gacgcagaca gttacaccgt
     7201 ttaaatgcaa taatcagcca cgtttctcgt taataacaat accagtacct ggtttgcgca
     7261 aggcgaagga ttatttttat gaagcttaag aacaccctcc tggcgtcggc actgctttct
     7321 gctacggcat tctccgttaa cgcagcaaca gaactgacac cggagcaagc ggcagcggtt
     7381 aaaccttttg accgtgtagt ggttaccggt cgttttaatg ctattggcga agcggtgaaa
     7441 gccgtttctc gtcgcgcaga taaagaaggt gccgcctctt tttatgttgt cgacacttct
     7501 gattttggta acagcggtaa ctggcgtgtg gtcgctgacc tctataaagc cgatgctgaa
     7561 aaagcagaag aaacaagtaa tcgcgtaatt aacggtgttg tcgaactgcc gaaagatcag
     7621 gctgttctga ttgaaccgtt tgacacggtc accgtccagg gcttctatcg tagccagcca
     7681 gaagtcaatg atgccatcac caaagcggca aaagcgaaag gtgcctactc tttctacatc
     7741 gttcgtcaaa tcgatgccaa ccagggcggc aaccagcgta ttactgcatt catctataaa
     7801 aaagatgcta agaaacgtat cgtccagagc ccggatgtga tcccggcaga ttccgaagca
     7861 ggacgtgcag ctctggctgc cggtggcgaa gccgcgaaga aagttgagat cccgggtgtt
     7921 gcgactaccg catcaccaag ttctgaagtc ggtcgcttct ttgaaaccca gtcatcaaaa
     7981 ggcgggcgtt acaccgtcac gctcccggat ggcactaaag tcgaagaact gaacaaagcg
     8041 acagcagcga tgatggtccc gttcgacagc atcaaattct ctggcaacta cggcaacatg
     8101 accgaagtct cctatcaggt tgcgaaacgt gccgcgaaga aaggtgctaa gtattaccac
     8161 atcacccgcc agtggcagaa acgtggtaat aacctgaccg tcagcgcaga tctgtataaa
     8221 taactattca gttactccta taaggcggct tgatgccgcc tttcgcattt ttagtcacaa
     8281 atttccccgc gaccattgca tgcagacaca tctcttcgta aaatcccgcg cttcagtggc
     8341 aaactccatt tttatgcgtt ttgccctaat atttattctt cttatcacgt tttaatcact
     8401 ggatatcgat ggaaaagaaa ctgggactga gcgcactcac cgcgctggta ttaagctcaa
     8461 tgctgggcgc gggtgttttc agtctgccgc aaaatatggc ggcagttgcc agcccggcag
     8521 cactgctcat cggctggggt attactggcg ctggcatttt attgctggcc tttgccatgc
     8581 tgatcctcac gcgcattcgt cccgaacttg atggcggtat ctttacctat gcccgcgaag
     8641 ggtttggcga gctaatcggc ttttgttccg catggggata ctggctgtgc gcggtcatcg
     8701 ccaacgtctc ctatctggtg atcgtttttt ccgcgttaag cttttttacg gacacgccgg
     8761 aattgcgcct gtttggcgat ggtaatacct ggcaatcgat agtcggtgca tcggctttat
     8821 tgtggattgt tcactttttg atcctgcgcg gggtgcaaac cgctgccagc attaacctgg
     8881 tggcgacact ggcaaaattg ttgccgctgg gtctgtttgt tgtgctggcg atgatgatgt
     8941 tcaaactgga taccttcaag ctcgacttca ccggacttgc acttggcgta cctgtttggg
     9001 aacaggtgaa aaacaccatg ctgatcaccc tgtgggtttt cattggtgtg gaaggtgccg
     9061 tggttgtttc tgcgcgtgcg cgtaataaac gtgatgttgg caaagcgaca ctgctggcgg
     9121 ttctctccgc tctgggcgtt tacttgctgg taacgctgct ttcgctgggt gtggtggcac
     9181 gtcctgaact ggctgaaatt cgtaacccgt caatggccgg tctgatggtg gaaatgatgg
     9241 gaccatgggg cgaaatcatc atcgctgccg gtttgatcgt ttccgtttgc ggcgcgtact
     9301 taagctggac catcatggcg gcagaagttc cgttcctggc tgccactcat aaagcattcc
     9361 cacgcatttt tgcgcgccag gacgcacaag ctgcgccatc cgcctcactg tggttgacca
     9421 acatctgcgt gcaaatttgt ctggtactca tctggctgac cggttccgat tacaacacgt
     9481 tgctgaccat cgcctcagaa atgattctgg tgccctattt cctggtcggc gcgttcttgc
     9541 tgaaaatcgc aacccgtcca ctgcataaag cggtaggtgt cggtgcctgc atttatggct
     9601 tatggttatt gtacgcctct ggtccaatgc acttgctgtt atccgttgtt ctgtatgcac
     9661 cggggctgct ggttttcctg tatgcgcgca aaacgcatac acatgataat gtactgaacc
     9721 gtcaggaaat ggtgctgatt ggcatgctgt taattgcctc agttccggcg acctggatgc
     9781 tggtgggata acgttcatcc catcgttgga tagcaaagga gataacgatg ggtaaaaccc
     9841 agcccttgcc aatattaatt actggcggag gtcgtcgcat cggcctcgcc ctcgcatggc
     9901 atttcattaa tcaaaagcaa ccggtgattg tcagctatcg gacacactat ccagccattg
     9961 atggactgat taatgcaggt gcgcagtgta ttcaggctga tttttcgacc aacgacggtg
    10021 tgatggcgtt tgccgatgaa gtactaaaaa ccacccatgg tctgcgtgct attttgcata
    10081 acgccagtgc gtggattgcg gaaaaaccgg gtgcgccact ggccgacgta ctggcttgca
    10141 tgatgcagat ccacgttaat accccatacc tgctcaacca tgcgctggaa agattactgc
    10201 gtgggcacgg acacgccgcc agcgatatca ttcactttac cgattatgtg gtggagcgcg
    10261 gtagcgacaa acatgttgcg tatgctgcaa gcaaagcggc actggataat atgacccgct
    10321 cgtttgcccg caagctggca ccggaagtga aagtgaattc tattgcgcca tcgctgatcc
    10381 tgtttaatga acatgatgat gccgaatatc gacaacaggc gctgaataaa tcactgatga
    10441 aaaccgcgcc tggcgagaaa gaagtgatcg acctggtcga ttacttactt accagttgct
    10501 ttgtcaccgg acgcagtttc ccacttgatg gcggtcgtca tctgcgttaa tgcagcttta
    10561 tccagcatat aatcaacacc catgcgctta tcccccaaca cgcaacagaa ccaacaaaag
    10621 cggcgggcaa gcgcatcatt ccggtgaaat accacagcga caccaggtaa acaaaatagg
    10681 gaataatcga ccacatacta aaaatgatgg ttgcgcgtaa ggcttcaatg ccgcgttcgc
    10741 tggcaacaat ataatgcgcg ataagcgcaa aggtcgggaa aagtggaatc agcccggcga
    10801 tataataatt tttctttttt gctaaaacac caatcaacag cactaccagc gcaccgagcg
    10861 cggctttgat taccagcccc atctttttac cttaacactt ccataacaag tcatcagtag
    10921 aatacctgat gaaaacttgt ttagaaacga ttgatagtaa gtaaaaacag cgcggtgtat
    10981 tgtgacgttt ttatatctac cgtgaatgtt atgaacacta tcgtatttgt ggaagatgat
    11041 gcggaagtcg gttcactgat tgccgcgtac ctggcaaaac atgatatgca ggttaccgta
    11101 gaaccgcgcg gcgaccaggc cgaagaaacc attttgcgag aaaatccgga tttggtgtta
    11161 ctcgacatca tgctaccagg caaggacggc atgaccattt gtcgtgattt acgcgcaaag
    11221 tggtctggac cgattgttct tctaacctct ctcgatagcg atatgaacca catcctggca
    11281 ctggaaatgg gtgcctgcga ctatattctc aaaacgacgc cccctgctgt tttgctagcg
    11341 cgtttacgtt tgcatttgcg tcagaatgag caagccacac tgaccaaagg tcttcaggaa
    11401 acgtctctga ctccctacaa agccctgcat ttcggcacgt taactatcga ccccatcaac
    11461 cgcgtagtca ccctggctaa cactgaaatc tcgctctcga cagctgattt cgaattattg
    11521 tgggaactag ctacgcatgc cgggcaaatc atggaccgcg atgcattgct gaaaaattta
    11581 cgcggcgtca gttatgacgg actggatcgt agcgtggacg tggctatttc gcggttaaga
    11641 aaaaaactgc tcgataacgc cgcagaacct tatcgcatta aaactgtgcg taacaaaggc
    11701 tatctttttg cgcctcatgc atgggatagt gacaataaac aatcagatga ctaatatttg
    11761 ttcatctgaa aaagagaaca attaacga
//
LOCUS       DAETPZ010000112        11596 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.112, whole genome shotgun sequence.
ACCESSION   DAETPZ010000112 DAETPZ010000000
VERSION     DAETPZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.112"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            139..1824
                     /gene="btsS"
                     /locus_tag="K0W59_003492"
     CDS             139..1824
                     /gene="btsS"
                     /locus_tag="K0W59_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HBI3024384.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            1821..2540
                     /gene="yehT"
                     /locus_tag="K0W59_003493"
     CDS             1821..2540
                     /gene="yehT"
                     /locus_tag="K0W59_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HBI3024385.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            2587..3057
                     /locus_tag="K0W59_003494"
     CDS             2587..3057
                     /locus_tag="K0W59_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBI3024386.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(3098..3559)
                     /locus_tag="K0W59_003495"
     CDS             complement(3098..3559)
                     /locus_tag="K0W59_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HBI3024387.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(3684..5687)
                     /locus_tag="K0W59_003496"
     CDS             complement(3684..5687)
                     /locus_tag="K0W59_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HBI3024388.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQELVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLNAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(5684..6820)
                     /locus_tag="K0W59_003497"
     CDS             complement(5684..6820)
                     /locus_tag="K0W59_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBI3024389.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(6813..9093)
                     /locus_tag="K0W59_003498"
                     /pseudo
     CDS             complement(6813..9093)
                     /locus_tag="K0W59_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9104..10192)
                     /locus_tag="K0W59_003499"
                     /pseudo
     CDS             complement(9104..10192)
                     /locus_tag="K0W59_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
     gene            complement(10734..11141)
                     /locus_tag="K0W59_003500"
     CDS             complement(10734..11141)
                     /locus_tag="K0W59_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024390.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            complement(11202..>11596)
                     /locus_tag="K0W59_003501"
     CDS             complement(11202..>11596)
                     /locus_tag="K0W59_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBI3024391.1"
                     /translation="RSEFAQLFADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKS
                     ATVGQLMGMRYKGWESGYEDAFVYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVY
                     RDNKSVTFAELDVFDLSEALSAPDVIFH"
BASE COUNT         2802 a         2943 c         3017 g         2834 t
ORIGIN      
        1 ctatagggtg attttatttt tgccaggcga ttttgagtga tcgtactcac gaattctcat
       61 ttttctgcaa gagttcaaag aaagttaaac gcaggcaatg tatgttacgc gttttaaagg
      121 gaagtgtggt ttgcgggtat gtacgatttt aatctggtgt tgctgctgct tcagcagatg
      181 tgcgtttttt tagtcattgc gtggttaatg agtaaaacgc cattattcat accgttaatg
      241 caggtcacgg ttcgtctgcc gcataaattt ctctgctaca tcgtcttttc catcttctgc
      301 atcatgggca cctggtttgg gttgcacatt gacgattcta ttgccaatac ccgtgcgata
      361 ggcgcggtaa tgggcggctt actcggcggt ccggtcgtcg gtgggctggt tggtctgacc
      421 ggcggcttac atcgatattc gatggggggc atgaccgcgc taagttgcat gatctcaacc
      481 atcgttgaag gattgctcgg cggcctggta cacagcatcc tgatccgtcg cgggcgcact
      541 gataaagtct ttaaccccat taccgccggt gccgtcacgt tcgtcgctga aatggtgcaa
      601 atgctgatca tccttgcgat cgcccgacct tatgaagatg cggtgcgtct ggtgagtaat
      661 attgctgcgc caatgatggt caccaatacc gtcggcgcgg cgctgtttat gcgtatattg
      721 ctcgataaac gcgcgatgtt tgaaaaatac acttcggctt tttctgccac tgcgctgaaa
      781 gtggcagcct cgacggaagg cattttgcga caggggttta acgaagtgaa cagcatgaaa
      841 gtggctcagg tgctgtatca ggagctggat attggtgcag tcgcgattac cgatcgagag
      901 aaattgctgg cctttaccgg aattggtgac gaccaccatt tacccggcaa accgatttct
      961 tcgacttaca ccttaaaagc gattgaaacc ggtgaagtgg tctacgctga tggcaacgaa
     1021 gtaccttatc gttgctcttt gcatccgcaa tgcaaactgg ggtcgactct ggtaattccg
     1081 ttgcgtggcg aaaatcagcg cgtgatgggc accatcaaat tgtatgaagc caaaaaccgt
     1141 ttattcagtt caataaaccg cacgctgggc gaggggattg cgcaactgct ttcggcgcag
     1201 attcttgccg ggcaatatga gcggcaaaaa gcgatgctca cccagtcaga aatcaaactg
     1261 cttcacgccc aagtgaatcc tcattttttg tttaatgcgc ttaacaccat taaagcggtg
     1321 atccgccgcg acagcgaaca ggccagccag ctggtgcagt atctttccac ttttttccgc
     1381 aaaaacttaa agcggccttc ggagtttgtt actctcgccg acgaaattga acatgtgaac
     1441 gcttatctgc aaattgaaaa ggcgcgcttc cagtcgcggt tgcaggtcaa cattgctatt
     1501 ccgcaagaat tatcccagca gcaattgccc gcgtttaccc tgcaaccgat agtggaaaac
     1561 gccattaaac atgggacatc acaactgctg gatacagggc gagtggcaat cagcgcccga
     1621 cgtgaggggc aacatttgat gctggagatc gaagacaatg ccggtttgta tcaaccggta
     1681 accaatgcca gtgggctggg gatgaatctg gtggataagc gtttacgtga acggtttggc
     1741 gatgactatg gaataagcgt cgcctgtgag cctgatagtt acacccgaat aacgttacga
     1801 ctaccatgga gggacgaggc atgattaaag tcttaattgt cgatgatgaa ccgttagcac
     1861 gggagaacct gcgtatattt ttgcaggagc agagcgatat tgaaatcgtt ggagagtgtt
     1921 caaacgccgt ggaagggatc ggcgcggtgc ataaactgcg cccggatgtg ctgtttctcg
     1981 atatccagat gccgcgcatc agtggtctgg aaatggtggg gatgcttgac ccggaacatc
     2041 gcccgtatat tgtttttctc actgcgtttg acgaatacgc tattaaagcc tttgaagaac
     2101 atgcctttga ttatctgctg aagccaattg atgaagcgcg actggagaaa acgctggcgc
     2161 gattgcgtca ggagcgcagc aagcaggatg tttcgctgtt accggaaaat caacaggcgc
     2221 tgaaatttat cccttgtacg gggcatagtc ggatttattt gctgcaaatg aaagatgtgg
     2281 catttgtcag cagtcggatg agcggtgtct acgttaccag ccacgaaggg aaagagggct
     2341 ttaccgaatt gacattacgt accctggaaa gtcgtacacc actactgcgc tgccatcgtc
     2401 agtatctggt taacctcgcg catttacagg agattcgtct ggaagataac ggccaggccg
     2461 agttgatttt gcgtaatggc ttaaccgtgc cggtcagccg ccgttatctg aaaagcttaa
     2521 aagaggcgat tggcctgtaa aagactgcta aaatggcttt ttgcctcatc aacacctgaa
     2581 ggcctcatga taagtaacga tattctgcgc agcgtgcgct acattttgaa agccaataat
     2641 aatgacctgg tgcgtattct ggcgctggat aatgtcgaag ccaccgcgga acagatcgcc
     2701 gtctggctac gtaaagaaga cgaagagggt tttcagcgtt gtccggacat tgttttgtcg
     2761 tcattcctca atggcctgat ttatgaaaaa cgcggcaagg atgagtctgc tccggcactg
     2821 gagccggaac gtcgcattaa taacaacatc gtgctgaaaa aattacgcat cgcgttttcg
     2881 ctgaaaaccg atgacattct ggctatcctc accgaacagc agttccgcgt ttcgatgccg
     2941 gaaattacag cgatgatgcg cgcaccggat cataaaaact tccgcgaatg cggcgatcaa
     3001 tttttacgtt attttctgcg tggactggca gcgcgccagc atgtgaagaa aagctaagac
     3061 gggtatggcg gccatgcgaa acatggccgc cgacagatta tttcacttct ttaaaaccag
     3121 cggctttcat caccagttcc atttgcgcca tagtgatacc ttttttggca tcttcagcag
     3181 aaacgttgat tcctgaaata ccctgcaggg ctttaaaatc cactttttcc atatcgatag
     3241 tcacgttttc ctgcgcgtag gtatcggtat aggttgattt ttcttcaaca cccgcgatgt
     3301 ttttgtattt ggcgcttaac ggctcaagtg tcttggcagc atcttctttg gtggttgcac
     3361 caatggaggc aaattgaatt ttggtttcag aagattgctt aagcaccttg tcacctttgt
     3421 agacataggt aatggcaatt tcagtgccgt tcagattggc gctgaatttc ttcgattctt
     3481 ctttgtcacc gcagccagca agagagaaaa ccagaacaga tgcaacaacg agggaaaaca
     3541 gcttattgaa agccttcatg taaaactcca ttttatttaa tcaagaaact ggtgactctc
     3601 accaggggct atataggata tgcctaatac cgtggcgtga gcagtccgga actggagtag
     3661 aactcttagt aaaaagcact atttcatcct tgttgctgaa gcatggggaa taattgttcg
     3721 caaagcaaaa caccgttatt cattgcttct acccgtgcct cgctttctgt attacgaaat
     3781 tgtgccaaca catgtgccag ccgataaaaa cccaccgcgg tgaggtcatt cgccagcaac
     3841 tctgcctgac taatagcgct ctgttcctga tagcgccagc cgttatggag cagttgaata
     3901 agtaacgcct ggcagcgcat cagcaactga tgagcagtag acggcacagg cagaacgctg
     3961 gcagaaggta gtgataccac cacaggcgca gtttctgcgt ccagcgccca ggcacgagtc
     4021 tttgtcatca ttacctgtgg ttccagtgtc aattgcccat caacaaaact gacaaagcca
     4081 gaaaccagac acacggggtc gtctgtttgt tgcaaaagcg ccgccatgcg ttcaatggca
     4141 taaggcgcgc tggctgaggc tggcagtgat aacgtcagca gattatcttc accttcgccg
     4201 ctgattacct gcgcatccag cgtctggcgg ctgctatccc aaccgagcga aatacactca
     4261 gcgaccggca gaataaataa gttatcgacc tgattaagag gccgtatgca ggcgggggga
     4321 cgctggcgta aatattcccg caaagccaca atgcccggct ggcgtaacgg cgcgttcaac
     4381 atttgccagg catcaggcga cagcggtaca acgctgctta agcggttgcg ggtagctaac
     4441 agcagctcgc catcggcact gcgttttgct gcttgtgaaa caatttgccc acccgccagt
     4501 gcgccagcct gaaaactaaa cagccgacgc gtagctgccg gtgagtcttc ctgttcactt
     4561 cgcggccaac tgcgcgaaag gtgcaaaata ctgccggtgt cgggatcggt aaaccagatg
     4621 cgtaaaccat aatgctcaat atcctgccag caacgcatac ctaaagacac cagccgcaga
     4681 tgatcaagct ttgcttctcc ggcaatgcca gagccaacga ccgtgcgcca cggcacagga
     4741 ggaacttcac caacactgtc gctccgggcc atctcttgtg cgcaatttaa tcgactgttt
     4801 aatgccgcaa gctgacgtaa gcattctccg gcatgatagt ggctggcgcg ggcgtggaag
     4861 gcatcaacgc tggcgcgcag ttgccgtagc gattcactca cccatcgcca gctgcagcgt
     4921 tccgctgctt gctgcgcgcg gctgaaagcg gcctcgtagt gaataagcgg ctggctgatg
     4981 ccgctcagcc ataatgcctg gcttagttgt tgaacatatt gacgacacgt tttgccttct
     5041 tcgctggcaa acggatcgtc agatgatgtg acgtgttcgc tgcgcatctg ccagattaaa
     5101 tgagtcaatt ccgcttgctg cgcttttgcc tggacaaagg cctgtactgc cagtacgaca
     5161 tgttcgcaaa gtgtgccttc aatacaatca caacgggcga aacgaatact gctgcgggaa
     5221 taaaaacgca catcgctcat cggtaagcgg gcagagggaa tttcacccgg cgcacagaac
     5281 aactcaatgg tgatgctttt agcgaccagc tcctgtgcgc gtttgcgggt ggcatcggga
     5341 agggtagcca gttcttccag ccagattgcc ggatcccatt cctcttcttt tcccgtaggc
     5401 tgggcggtag cacaaagtcg ttgataactt aacaccagca tcacgcgatg acggcacatg
     5461 ccgctggccc cacaggtgca ctgagcctct ttcagtgcct ggccgttcgc cagctgggta
     5521 cggacaccgt cactgaaggt ggcgattaaa gcgctgttct catggctgat ttccgggacg
     5581 ttgccatttt ccagttcctt aaggctgcgc ttaacaaaac cggcattgct taacgccgtc
     5641 agtgcctgcg gtgtcagttc taataattcc ggacgtagtg aattcatgac tgaagattct
     5701 ccgcaagcca tgatgccagc tcgcccggtg tcatggcggc tatttgtgcg ccgacattaa
     5761 ccagcgcctg ggccgtatcg cggtcatagc aaggtgttgc tgtgctatcg agcgctgcca
     5821 gtcccagcac tttgatgccg ctctggacac actttttcac ttggtgcgtc agcaatgatg
     5881 atgaaccccc ttcataaaaa tcgctcacga ggataatgac gctttttgca ggttgttcaa
     5941 taagttgccg accatactcc atggcactgg cgatattggt tccgccgcct aattgcactt
     6001 tcattaataa ctctaccgga tcggcaacgt ctgccgtgag atcaacgacg cttgtgtcaa
     6061 acgccaccag atgggtacga atgccgggta actgccataa acaggccgcc atcaccgcag
     6121 agtggatcac cgaatcgacc atcgatccgc tttgatcaac cagtaagacc agttgccatt
     6181 gctcgctttg gcgtttaatg cggctgttaa agcgggggaa ttcgatatac aacttgccgt
     6241 gttgcgggtg ccagtgttgc aggttggcgc gcagagtact tttgaaatca aagtttcgcg
     6301 ccagtggaat aaatgagcgg cgacgacgat cgcggacacc agaaaaagcc tgacgaactt
     6361 cctttgccag tcgcgccata atttcttcca caacctggcg cacaatcctg cgggcggcag
     6421 ccagtacttc ggggttcatc agatgtttgg tgtgcaaaac ggcgcgtagc aggctttcgg
     6481 aaggctgcat acgttccagc acgtcgagat ttgtcaccac atcttcaatg ccgtagcgca
     6541 gtacggcatc gctttccagc cgctcaatca cctgttgcgg aaacagcgtg tgaatactgt
     6601 tgatccactc aggagtggtg agatttgagc cacctaatcc actagagcgt tcaccacgct
     6661 ggagccgttc aggatcgcgc ccatacagcc actccagcgc gtgatctatc tgccgggcgt
     6721 tgtcatccag cccacaaagc gtcgtttctg ccgcttcgcc aagaattaat cgccagcgtt
     6781 gtagctcacg ggtggtcaga agatcgttca gttcagacat ggaaaactcc ccagtgttgc
     6841 agcgatgcca gtgcctgctg ttcgagttgt tgatgatgtg caatggcttg cggtggacaa
     6901 tgcaacggca tttgcagtgc cgaaacggga agttgcgcca gttgataatg ctcaagcacc
     6961 tgatgcgcca gcgtcccgcg ttctcgtggc ggtagccagg ccattgccgc gcgtaaatcg
     7021 ggcagggcat tggtaaaatc ggcatcactt agttgattta aatgactgct gaaaccggcg
     7081 ataaatgccg gctgacaggc cagttgatga cgggctaatg ccagcaaacc gtgcagcgcc
     7141 tcgccggatt gtgccgggga taactgcgcc agcatcgtca gcgccgcttc ggcactagcg
     7201 ttgggatgtt ccagacgcat cagcgcacca agagtcgcgc cgcgatccag tgccaggaca
     7261 tgaatcgcct gactgtgtcg ctccagcaag gctaccgccg cagaaagcga aacgccgggg
     7321 agttgcacac cgctatgtag atcgcgcagg atatgacaca acgcttgcca gctatgcaag
     7381 tgagcgtgaa actccttttc atccggtctg ccgttggatt cacacagcca cagcgtgcga
     7441 tcgatagccg cgcataacgt cgtttgtaat atctgcgcgc cttgcatacc gctaatttca
     7501 tccagccgcc ataaggcata tagcacttcc agcgcctggc ccatttcggc aaattgattt
     7561 tcctgggcga ttaataatgt gagttgctcc agtaattgtt gactgaagga cgccagaccc
     7621 gctgacgccg cctggctaag acatgtggta atgctgccga ttccacccgc gtccagcata
     7681 tcggcttcta atttatggcg tgcggcttcc tggagtgtgg caccaaagca ggcggcctca
     7741 atcaatgccg catgctggct aagcgggcgg gttaatttcc agcgttcttc accgttgcct
     7801 gcaagtgtca gtgtgcttcc ctgctggcgt acaatccctg ggatctccag tattgccagc
     7861 cgatgtaaca cctgactttg cgctagtcca ttggggttaa agcgatttaa ggtaagctcc
     7921 gccggaagag agattgcggt acgttccagt tcgcaagtga catcttttgg cagaggcggt
     7981 tgtggcgtag aaggggcaag tttaccgaat ccgtcacccg ctaatgtgtc tatcaacgtt
     8041 agcagaatcg gatcggtatc gggatgaata acgccgcgat agctccacgg caacggcgca
     8101 ttcagggctt ctttaatcag cgatcctgct atggcatcca gccagtcagt gcgtaacggt
     8161 agtgtatgac cgcgcaactg tgccagtgcc atcgcatgca gatgagcggc agccatatcc
     8221 gcggtcgaag caggcaactt gtgctggcgc aaacgggtga gaaccgtttt tagcagttgt
     8281 tctcccgcct gctgtaagcc ccactgccag caccagtttt gccagaccgg ggcaggcatt
     8341 cctgaaaggt atcctgccag cacatcaagg cgcttttcac tgtagggggg tgagataaca
     8401 acctgtaacg gcatctgcca gcgacggcaa ttctggcgtg ttaattacct gcggacattc
     8461 gcgccacatt tttgccagtg ccggagcgtg ccagccaccg cagacaacta acacgtcgcc
     8521 attattttgc tgcatcgccc atccaatcca gcggaccata aaggcttcgc gctgacgatt
     8581 gagagcatcg ccgggggagt cgccccgcag ttgggcaaaa tagtgcgcca gtgcagaggg
     8641 taatgcagtt tgctggcttt catcttcgaa caagtgatcc cacagggtgt cgctgttatc
     8701 catgcgggtg gcacgcagca gcaaggtctg gctttcctct tgcgtatcag gcgaatcgtc
     8761 ctcttcttca ctctgcgccc agcaaggcaa atcgatgaag taagtttgtg cctgaatacg
     8821 acgatcggct tgtagcgcct gccactccgg cgaaaattca gcaaatggcg tccaggcacc
     8881 acgcccgggg gctgcaccgt cctgatactg gcaataactg taaatagcta ccggaagctg
     8941 gtgggctaaa aacagttcgt ctacccggtc attaaaatca gccgggcctt caatcaacac
     9001 gtatcgtggc cgctggcttt cgattaacga tttcaccagc cgggcgcagg ccggactatg
     9061 atgccggatg ccgacaatta acggctcgct catgcatctt tcctcacggc aggcggtggc
     9121 gggcttgata ataagcctgc cagtgagctt ctttatgtgt ggcgacacgc tgttcgaagt
     9181 aacggcgcag acgagcgcga tcttcttcgt tatctttgac gatggttccg gcaatgcagt
     9241 ccaccaggtc ggctggctca cctgcgcgat tcgctaaaaa ccaggcgcgg acgcctacgg
     9301 cgtgggccac gttcacggct tcagcggtgg acataatcgc tgttagtgta tccattgacg
     9361 ttttcttttc gccgttggca cgcagatcgc ggaaggtgcg gaccagtaac tccagtaccg
     9421 cgtcaggcac tttatgcgga ataccgctat gggccagcaa acgcgccgaa gcactggcga
     9481 caagctccag ctcctgggcg aaatccataa tcggaaagac agtttcaaaa tcgaagcgac
     9541 gctttagcgc agcactcatt tcattgacgc cgcgatcgcg ggtattggca gtggcaataa
     9601 tattaaagcc ttctcgcgca tagagctgac tggcttcacc agtgagttcc ggcaccgtca
     9661 tcaccctgtc agaaagcata cctaacagac agtcttgcac ttccagcggt gtacgcgtta
     9721 tctcttcaaa acggacgatt ttgccgtcgc gcattccctg gtaaagcggg gcagggacca
     9781 gcgcttccgt tgatggacca tgattgataa gcagggcgta gttccagccg tacttgattt
     9841 gatcttcggt agtagatgcg ccgccctgga tggttaaccc ggcatcgccg ctaatagagg
     9901 ttgccagtaa ttcagacagg agagattttg cggtgctagg ttcccccacc aacatcaaac
     9961 cgcgtccggt ggcaagagtg actaacatac gttcgacagc ggagggacta atcaccactt
    10021 tacggctaat attttgcgcg ctatcgccaa ggataaaagc acgggccgca ggcaaactta
    10081 actaccaacc gggcgggcaa ggtgcgttat cattttgttt taattttgcc agttcatcgg
    10141 cgtataacac agcagcaggc ggacgctgca gatgattatt ctgcggtgac atatattcat
    10201 ccctgaaatg tattaatgaa ataaacgata ttttacgcgc aataaatgtt tggcgcatta
    10261 gttgtttgag cgaaaaatat ctggaaagca gatcacagta aataaaaaaa cctgctggtg
    10321 aggcaggttc ttttatttac tattttcagg cctaacacca ctgcctgtgc aacatcgtgt
    10381 tccaacgaac gaattataac cacctctgga cagtgttgca attaagataa tgagatttat
    10441 aatcctgact gttgggaagt tgcgccggat cgaacccatt tcaagtcctg gctaccggac
    10501 gggtggtgcc gcaggtagtg ctctgatccg gagtgcaaca tggcgactca acaaacgtaa
    10561 accagtatcg acaggcagtg taagggtaag gtgctggtgt tgtgctcctt aataaggaga
    10621 gcggatggac acgctgacac aaaagttaac cgtgctcatt gctgtactgg agctattagt
    10681 ggctctgtta cggttgattg atttgttgaa gtaacgggca aattgaaaca catctaaggg
    10741 ggaataccgt tccccccttt aataaagcta tactcgtcat acttcaagtt gcatgtgctg
    10801 cgacttgaat tatctagagt atataattct atttgttggt tgaatatatt ctgtagttta
    10861 caattaaaaa ttactattac aggttcaggt gattcgcctc gtaatttttg ctcatcaata
    10921 aactcatctc ttatataaat caacgcatca tttattagcg ggtcaacatc ctctcgatca
    10981 cgttgatgaa atgttggacg tagccggaac cgttccagtc ggtagacgga acctgtaaat
    11041 aattcagagc atttatggat ctcaagtctg aaggaaaacg gatcttcagg tacagggacc
    11101 tcataatcgt aaatagcaac cagctctttt tgcacgatca tcgctttccc cgctggtaag
    11161 tcgtgtagat aacgggcagt cgcatcactg cccgctttgt tttaatggaa aatgacgtca
    11221 ggtgcgctta acgcctcact caaatcaaac acatcaagtt cggcaaaagt gacggattta
    11281 ttgtcacggt acactcgaag agaacggaag ctcattagcg ctttgctatc aacattgtag
    11341 tggttaaaac cgggtgaaaa cttaaggacc agccggtact cacccaggtc atagacaaat
    11401 gcgtcctcat aacctgactc ccagcctttg tatcgcattc ccataagttg cccaacggta
    11461 gcggatttac cttcccagcg agtcaggctg ttactggttg actcgtcagg tgtgagcagc
    11521 accgtgcggc gcgacaactg gcgaaaaggc ggcataattt cgtaatcggc aaaaagctgt
    11581 gcaaattcac tgcgca
//
LOCUS       DAETPZ010000113        11447 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.113, whole genome shotgun sequence.
ACCESSION   DAETPZ010000113 DAETPZ010000000
VERSION     DAETPZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.113"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            17..469
                     /locus_tag="K0W59_003502"
     CDS             17..469
                     /locus_tag="K0W59_003502"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI3024392.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            715..1759
                     /locus_tag="K0W59_003503"
                     /pseudo
     CDS             715..1759
                     /locus_tag="K0W59_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1821..2435
                     /locus_tag="K0W59_003504"
                     /pseudo
     CDS             1821..2435
                     /locus_tag="K0W59_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(2492..3949)
                     /gene="pepD"
                     /locus_tag="K0W59_003505"
     CDS             complement(2492..3949)
                     /gene="pepD"
                     /locus_tag="K0W59_003505"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBI3024393.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            4211..4669
                     /gene="gpt"
                     /locus_tag="K0W59_003506"
     CDS             4211..4669
                     /gene="gpt"
                     /locus_tag="K0W59_003506"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBI3024394.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4761..6005
                     /gene="frsA"
                     /locus_tag="K0W59_003507"
     CDS             4761..6005
                     /gene="frsA"
                     /locus_tag="K0W59_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBI3024395.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            6063..6464
                     /gene="crl"
                     /locus_tag="K0W59_003508"
     CDS             6063..6464
                     /gene="crl"
                     /locus_tag="K0W59_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBI3024396.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(6503..7558)
                     /gene="phoE"
                     /locus_tag="K0W59_003509"
     CDS             complement(6503..7558)
                     /gene="phoE"
                     /locus_tag="K0W59_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBI3024397.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            7846..8949
                     /gene="proB"
                     /locus_tag="K0W59_003510"
     CDS             7846..8949
                     /gene="proB"
                     /locus_tag="K0W59_003510"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBI3024398.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDHNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            8961..10214
                     /gene="proA"
                     /locus_tag="K0W59_003511"
     CDS             8961..10214
                     /gene="proA"
                     /locus_tag="K0W59_003511"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBI3024399.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            10329..10404
                     /locus_tag="K0W59_003512"
     tRNA            10329..10404
                     /locus_tag="K0W59_003512"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10362..10364,aa:Thr,seq:cgt)
     gene            complement(10419..>11447)
                     /locus_tag="K0W59_003513"
     CDS             complement(10419..>11447)
                     /locus_tag="K0W59_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBI3024400.1"
                     /translation="AWRVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWML
                     HFREAILSDITTEKIMEAVDGMENRRHRLNWEMSRDRCLRLGNPVPEYKPKLASKGTK
                     TRHLAILRAILNMAVEWGWLDRAPKISTPRVKNGRIRWLTEEESKRLFAEIAPHFFPV
                     VMFAITTGLRRSNVTDLEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQ
                     GLHKRWVFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWA
                     SWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNTAQEE
                     NVVYLNAR"
BASE COUNT         2748 a         2838 c         2947 g         2914 t
ORIGIN      
        1 tttttgaatt tatattatga ataacataca aataagagat tatcagcctg gcgattttca
       61 gcaactatgc gctattttca ttagagcggt tacgatgacc gtcagtcagc attattcacc
      121 acaacaaatt gccgcctggg cgcagattga cgaatctcgc tggaaggaga aactcgcgaa
      181 atcacaagtg cgggttgcag tcattaatgc aaaactggtc ggctttatta cctgcgttga
      241 acactatatc gatatgttat ttgttgaccc tgaatacacc cgccgtgggg ttgccagcgc
      301 tttgttaaaa ccttttatta agtctgaatc cgaacttacg gtggacgcaa gcataaccgc
      361 aaaacccttt tttgaacgtt atggttttca gacagttaag cagcagcgcg ttgaatgccg
      421 gggagcgtgg tttattaatt tttatatgcg ttataaaccg caacattaaa tccagcttgc
      481 aatgaaaata acgcccgcct agtatgtgcg cgttttctta ttccacaaac tgcaaggagg
      541 taaatcatga caaatccttt atcaatgact ctttgcagac ctttccagga ttaattcttt
      601 ttttcttgcc ctggattcgt ctgccatttc ctgatttttt aattgttact ctcaataatt
      661 caagtcgcag cacatgcaac ttgaagtatg acgagtctat ttacggaatg gtatatgggc
      721 aattatattc gtcccttatc cgatgcggta tttaccatcg catctgatga cctgtggatc
      781 gagagtttag cgatccaaca attacacacc acggcaaatt tacccaacat gcagcgcgta
      841 gtggggatgc cagatttaca ccccggacgc ggctacccga ttggcgcagc gttcttctcc
      901 gcaggccgtt tttacccagc actggtcggc aatgatatcg gctgtggcat ggcgctatgg
      961 caaacagata ttctcgctcg caaatacaac gccgataagt ttgaaaagcg attatcagct
     1021 ttggatgacg ttgctgaaga aagctggctg gaggaaaacc tgccgtcagc atttgcacag
     1081 catccgtggc gcagctcgct tggctctatc ggtggtggca atcatttcgc tgaactgcaa
     1141 caagttgatc aaatcatcaa cgctgaactg tttgcactgg caggtctgga tgcgcagcat
     1201 ctgcaactgc tggtccatag cggctcgagg gagctagggc agtctatttt acagcggcat
     1261 attgcctcgt tttcgcatca tggtttgcct gaaggcagtg acgacgcgct ggcgtttgcg
     1321 cgttttaatc gccacctgat cgctttgcgc ataatgcaac aggttaaggc caccggaagt
     1381 ccggttctgg atgtggcgca taactttgtt agcgcgtgtc gaatcggtga tcaacaaggg
     1441 gtcttacatc gtaaaggtgc cacgcctgat gactgtggtc tggtggttat tcccggttca
     1501 cgcggtgatt actccaggct ggttcaggcc cgtcaggagt gaggaaacat tgcattcgct
     1561 ggcgcatggg gctgggcgta aatgggggcg caccgagtgt aaagggcgtc tggcagcgaa
     1621 atacacagcg acgcaactct cgcgtactga acttggcagt cgggtaattt gtcgcgataa
     1681 acaactcatc ttcgaagaag cgccacaagc ctataaatcg gctgaaagtg tggtgcaatg
     1741 tctgggcagg ccggattaat tattcctgtc gcgcggctgc gtccggtgct aacgctcaaa
     1801 aacagtggag ggaaaaaagg atgatcttgc tataactctc ctcagcgcag gggccggaag
     1861 aatgttgtct cgcagtgaaa aaggcgctgg acaggctaat taaagaagct gcccgacaag
     1921 acgtcgcggt aacggtgctg gaaacagaaa caggccgcta ctctgacacg ctgcgttcgg
     1981 cgctgatttc tctggatggc gacaacgcat gggcgttaag cgaaagctgg tgtggcacta
     2041 ttcagtagat ttgcccgagc ccgtatcgcc cacatcacgg gcgtaaaaac tggtttctgg
     2101 gcattgggcg ttttaccgct gatgagcagg agcaatccga tgcgatccgg tatgagacgc
     2161 tgcgttcgtc ggggccgggc ggtcaacatg tcaataaaac cgactcggcg gtacgcgcca
     2221 cgcatttggc atccggtatt agcgtgaagg ttcagtcaga gcgcagccag catgctaaca
     2281 aacggctggc gcgattgctg attgcctgga agctggatca gcagcaatag gaaaatagcg
     2341 cgtagctgaa atcacagcgg cgaatgttcc atcaccagat tgaacgtggc aacccgcgac
     2401 gaacgtttac agggatggct tttatcgaag gataatgaag gatgaaactg ccggaaggcg
     2461 attaaacgcc atccggcagc aaatcaaata attacttcgc cggaatttct ttcagcagtt
     2521 cagtcagcag tgtccagtaa tgacctacgc tttcgatgtg aacttgctca tccggagagt
     2581 gtggaccggt gatagttggc ccgatagaaa ccatgtccat ttccggatac ggttttttga
     2641 acagaccaca ttccaggccc gcgtggataa tctggatgtt cggcgtcttg ttgaacaggc
     2701 gctgataggt ttcacgtacc agatgcatca ccggagaatt agcgtccggc tgccagccag
     2761 gatatgcgcc tttcgcttcg gttttcgcgc cagccagttt acccagcgaa tccagcatgc
     2821 tcaccacgta gtctttaccg ctgtcgatca gtgaacggat caggcagtga atttctacgt
     2881 tattgtcagt catggtcacc acaccgacgt tcagggaggt ttcgaccaca ccttttgcca
     2941 cgtcggaatt gcggatcaca ccgttcgggg tggcgttcag cagacgaata aaggtatcgc
     3001 gagatttcgc aatcagggca gctttatcgt tcgctacaga gtccagcaac aaggccagat
     3061 ttttctcttt ctctgccagc tcgtttttca ggatctcctg ataggtattc accagagatt
     3121 tcagtgcgtc gactttatca gctgtgacag caatggtcgc aaaggcttca cgcgggatgg
     3181 cgttacgcag tgtgccgccg ttgaaatcga taaggcgcag gtccagttct tctgcatgac
     3241 ccgccaggaa gcgcaccagc agtttgttgg cattacccag gccaacgtgg atttcaccgc
     3301 cggagtgacc gcctttcaga ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa
     3361 ccgcttcacg atctaaatgc aggttggagg tgaagtcgat accccccgca caacccatgt
     3421 agatttcacc ttcttcttcg gagtcggtgt taatcagaat atcagcctgc aaccagttgc
     3481 tctgtaagcc gaacgcaccg tccataccgg cttcttcggt catggtcagc agcacttcca
     3541 gcgggccgtg aaccacgttt tcgtcagcca gaaccgccag tgcagaggcc ataccaatgc
     3601 cgttatccgc acccagcgtg gtgccgcgcg ctttaaccca ttcgccatca atataaggct
     3661 ggataggatc tttcgtgaag tcatgcacgg tgtcgttatt tttctgcggc accatatcga
     3721 ggtgggcctg taagacgacc ggtttacgat tttccatacc tgcggtagca ggtttacgaa
     3781 tcaggatatt acctacctga tcgcgttcga catggaaacc tttctctttt gcccaaccaa
     3841 caatgtattc agcgagttgc tcttcatgat aggacgggtg aggaatagaa cagattttgg
     3901 caaaaatatc ccacagcggc tgtggagata attgagacag ttcagacacg ttaagtctcc
     3961 ttgtcgatca cccgcaaaac agtattgcag gtcacagggt tagcagaaaa tgttgtcaac
     4021 acaagacagg cttgcgagat atgtattgag aataccactt tatcccgcgt cagggagagg
     4081 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatatcta
     4141 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
     4201 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
     4261 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
     4321 tggcggtctg gtaccggggg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
     4381 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
     4441 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
     4501 tgttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
     4561 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggatcgaaca
     4621 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
     4681 cctggcattg ccgggcgttg ttctttttaa cttcaggcag gttacaatag tttccagtaa
     4741 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
     4801 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggt gcacaaccgc
     4861 ctgtgcagtc ggctcttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
     4921 tgatgtggat ctggcgcggt attgacccac gtgaaatcct cgacgtccag gcacgtattg
     4981 tgatgagcga tgctgaacgt accgacgatg atttatacga taccgtgatt ggctaccgtg
     5041 gtggcaactg gatttatgag tgggccaccc aggcgatggt gtggcaacaa aaagcctgta
     5101 cggaagaaga tccgcaactc agtggtcgac actggctgca tgcggctacg ttgtacaaca
     5161 ttgccgccta tcctcatctg aaaggagatg acctggccga gcaagcgcag gctttatcaa
     5221 accgcgccta tgaagaggcc gcccagcgtc tgccgggcac gatgcggcag atggagttta
     5281 ccgtacccgg cggtgcgccc atcaccggct ttttgcatat gccgaaaggc gatggcccgt
     5341 tcccgacagt attaatgtgt ggtggtctgg atgcgatgca gacggactat tacagcctgt
     5401 atgaacgtta ttttgcgccg cgcggcattg cgatgctgac tattgatatg ccgtcggtgg
     5461 gcttttcttc aaaatggaag ctcacccagg actccagcct gttgcatcag cacgtcttaa
     5521 aggcgctgcc taacgtaccg tgggtggatc acactcgcgt cgcggccttt ggtttccgtt
     5581 tcggcgctaa cgttgccgtg cgtttggcat accttgaatc gccgcgtctg aaagcggttg
     5641 cctgtcttgg tccggtagtt cataccctgt tgagtgattt taagtgccag caacaggtgc
     5701 cggaaatgta tcttgacgtt ctggcgagtc gtttggggat gcatgatgct tccgacgaag
     5761 cgttacgcgt ggagctgaat cgctattcat taaaagtgca aggattgctg gggcgtcgct
     5821 gcccaacgcc aatgttatca ggctactgga agaacgatcc gttcagcccg gaagaggact
     5881 cacgcttaat cacctcatca tctgctgacg gtaaattatt agagatccca tttaacccgg
     5941 tgtatcggaa ttttgacaaa ggtcttcagg aaatcaccgg ctggatcgaa aaacgcttgt
     6001 gttaaaaatt tgctaaattt tgccaatttg gtaaaacagt tgcatcacaa caggagatag
     6061 caatgacgtt accgagtgga cacccgaaga gcagattgat caaaaaattt accgcactag
     6121 gcccgtatat tcgtgaaggt aagtgcgaag ataatcgatt ctttttcgat tgtctggctg
     6181 tatgcgtcaa cgtgaaaccg gcaccggaag tgcgtgaatt ctggggctgg tggatggagc
     6241 tggaagcgca ggaatcccgt tttacctaca gctaccagtt tggtctgttc gataaagcag
     6301 gcgactggaa gagtgttccg gtaaaagata ctgaagtggt tgaacgactg gagcacaccc
     6361 tgcgtgagtt tcacgagaag ctgcgtgaac tgctgacgac gctgaatctg aagctggaac
     6421 cggcggatga ttttcgtgat gaaccggtga agttaacggc gtgagtgaaa tgtgccggat
     6481 gcatcacatc cggcaatatt cattaaaact gatacgtcat cccaaccgcg acagtatcat
     6541 cattattaat attcagctta ttatcgctat ccagtttgtt gattttataa tcaacaaacg
     6601 ctgacatatt tttgttgaaa taatacgtag caccgacgtc gatataattg accagatctt
     6661 catcaccgat gccctcaata tctttccctt tcgataagac ataacccagc gatggacgca
     6721 gaccaaagtc aaactggtat tgagcgaccg cttcaaagtt ctgtgtctta ttggcaaagc
     6781 cgccagttat tggcgtcatt ttgcgtgttt cagaatagaa agttgccaga taaatattat
     6841 tggcatcgta tttcagacct gttgcccatg cttctgcacg cttgcctgtg ccacggcttt
     6901 gcaggttctg ctcgttggtg cgatctgagt tggtataggc cccactaatg gcgaaatcgc
     6961 tgccgccaaa gtcatatgtc aatgacgtgc cgaagccatc gccgttttgc tttttaacgt
     7021 cgcggttttc gtttttccct tgatattgca gggttaagtt caggccatcg ataacgccga
     7081 agaagtcggt gttccgatac gtcgccagac cgctggcgcg tttggtcata aagttgtcgg
     7141 tctgcgccga ggaatcgcca ccaaattccg ggaacatatc ggtccaggct tccacgtcat
     7201 acaacgcgcc caggttacga ccataatcga aagaacccaa atctttatat ttcaacccgg
     7261 caaaagcgag acgcgttttt tgctgtgcag tatcactctc cgctttattt ccggcaaact
     7321 ccgcttccca ccgaccataa ccagtcagtt gatcgttaat ttgagtttca cctttaaaac
     7381 caaaacggat ataactctgg tcgccatctt tactggcgtt atcactcata taatgcatgg
     7441 ctttaacttt gccatagaca tccagtttat taccgtcttt attatatatt tctgcagcct
     7501 gtacagatgc agatgccaca atgcccatta ccactaatgc cagagtgctc tttttcattt
     7561 tcattcctga ttttaattaa cgcgcgaata ttcagcggga gagccccgtt gaaaacagga
     7621 aagtttttaa cctgagattg ttaaagatat attacagatt aatgatattc tgaaaatgtg
     7681 gtaatttatt aaatctgtaa taaaagcgta aacaactgcc gctacgcttg ctgatcccgc
     7741 gcaacaaaac gccatgcttt gctcgcagat ggttggcaac cgacgacagt cctgctaaaa
     7801 cgttcgtttg atatcatttt tcctaaaatt gaatggcaga gaatcatgag tgacagccag
     7861 acgctggtgg taaaactcgg caccagtgtg ctaacaggcg gatcgcgccg tctgaaccgt
     7921 gcccatatcg ttgaacttgt tcgccagtgc gcgcagttac atgccgccgg gcatcggatt
     7981 gttattgtga cgtcgggcgc gatcgccgcc ggacgtgagc acctgggtta cccggaactg
     8041 ccagcgacta tcgcctcgaa acaactgctg gcggcggtag ggcagagtcg actgattcaa
     8101 ctgtgggaac agctgttttc gatttatggc attcacgtcg ggcaaatgct gctgacccgt
     8161 gctgatatgg aagaccgtga acgcttcctg aacgcccgcg acaccctgcg tgcgttgctc
     8221 gatcacaata tcgttccggt aatcaatgag aacgatgctg tcgctacggc agagattaag
     8281 gtcggcgata acgataacct ttctgcgctg gcggcgattc tggcgggtgc cgataaactg
     8341 ttgctgctga ccgatcaaaa aggtttgtat accgctgatc cgcgcagcaa tccgcaggca
     8401 gaactgatta aagatgttta cggcattgat gacgcactgc gcgcgattgc cggtgacagc
     8461 gtttcaggcc tcggaactgg cggcatgagt actaaattgc aggccgctga cgtggcttgc
     8521 cgtgcgggta tcgacaccat tattgccgcg ggcagcaagc cgggcgttat tggtgatgtg
     8581 atggaaggca tttccgtcgg tacgctgttc catgcccagg cgactccgct tgaaaaccgt
     8641 aaacgctgga ttttcggtgc gccgccggcg ggtgaaatca cggtagatga aggggcaact
     8701 gccgccattc tggaacgcgg cagctccctg ttgccgaaag gcattaaaag cgtgactggc
     8761 aatttctcgc gtggtgaagt catccgcatt tgcaacctcg aaggtcgcga tatcgcccac
     8821 ggtgtcagtc gttacaacag cgatgcatta cgccgtattg ccggacacca ctcgcaagaa
     8881 attgatgcaa tactgggata tgaatacggc ccggttgccg ttcaccgtga tgacatgatt
     8941 acccgttaag gagctggccg atgctggaac aaatgggcat tgccgcgaag caagcctcgt
     9001 ataaattagc gcaactctcc agccgcgaaa aaaatcgcgt gctggaaaaa atcgccgatg
     9061 aactggaagc acaaagcgaa atcatcctca acgctaacgc ccaggatgtt gctgacgcgc
     9121 gagccaatgg ccttagcgaa gcgatgcttg accgtctggc actgacgccc gcacggctga
     9181 aaggcattgc cgacgatgta cgtcaggtgt gcaacctcgc cgatccggtg gggcaggtaa
     9241 tcgatggcgg cgtactggac agcggcctgc gtcttgagcg tcgtcgcgta ccgctggggg
     9301 ttattggcgt gatttatgaa gcgcgcccga acgtgacggt tgatgtcgct tcgctgtgcc
     9361 tgaaaaccgg taatgcggtg atcctgcgtg gtggtaaaga aacctgtcgc actaacgcgg
     9421 caacggtggc ggtgattcag gacgccctga aatcctgtgg cttaccggcg ggtgccgtgc
     9481 aggcgattga taatcctgac cgtgcgctgg tcagtgaaat gctgcgtatg gataaataca
     9541 tcgacatgct gatcccgcgt ggtggcgctg gtttgcataa actgtgccgt gaacagtcga
     9601 caatcccggt gatcacaggt ggtataggcg tatgccatat ttacgttgat gaaagcgcag
     9661 agatcgctga agcattaaaa gtgatcgtca acgcgaaaac tcagcgtccg agcacatgta
     9721 atacggttga aacgttgctg gtgaataaaa acatcgccga tagcttcctg cccgcattaa
     9781 gcaaacaaat ggcggaaagc ggcgtgacat tacacgcaga tgcagctgcg ctgacacagt
     9841 tgcaggcagg ccctgcgaag gtggtggcgg ttaaagccga agagtatgac gatgagtttc
     9901 tgtcattaga tttgaacgtc aaaatcgtca gcgatcttga cgatgccatc gcccatattc
     9961 gtgaacacgg cacacaacac tccgatgcga tcctgacccg cgatatgcgc aacgcccagc
    10021 gttttgttaa cgaagtggat tcgtccgctg tttacgttaa cgcctctacg cgttttaccg
    10081 acggcggcca gtttggtctg ggtgcggaag tggcggtaag cacacaaaaa ctccacgcgc
    10141 gtggcccaat ggggctggaa gcactgacca cttacaagtg gatcggcatt ggtgattaca
    10201 ccattcgtgc gtaaataaaa ccgggtgatg caaaagtagc catttgattc acaaggccat
    10261 tgacgcatcg cccggttagt tttaaccttg tccaccgtga ttcacgttcg tgaacatgtc
    10321 ctttcagggc cgatatagct cagttggtag agcagcgcat tcgtaatgcg aaggtcgtag
    10381 gttcgactcc tattatcggc accatttaaa tcaataagtt acctcgcatt taagtaaacc
    10441 acgttctcct cttgtgccgt atttgtgcca ttgcgactta taatcgcatc gattttgctt
    10501 gcgtgctcgg tgagatgccc ggctgaaagg tgggcgtatc tttgaaccat ttcgagagtt
    10561 tcccatcctc ccatctcttt aagtgcaaga agagagacac cggactgaac cagccagctt
    10621 gcccaggtat gcctcagatc atggaagcgg aagttgctaa tgcctgcccg ctttaacgct
    10681 cccttccatg ccttgttgct gtcggttctc atcttcctta ccgctgctgt ttttgttccg
    10741 tcgcttcggt aggcaggttt ggtgtggaca aatacccatc tcttatggag cccctgctgt
    10801 tttcttaata tctggcatgc ggtttcgtta agaggaactc cgatcgcatt gccagctttt
    10861 gtttcatcag ggtgcatcca tgccattttc ttatccagat cgacctgtga ccactcaagg
    10921 tctgtaacgt tggaacggcg aaggcctgtc gtgattgcaa acatgaccac agggaagaag
    10981 tgaggagcaa tttctgcaaa caggcgcttc gattcctcct ctgtaagcca tctgatgcgt
    11041 ccattcttaa cgcgtggtgt tgatattttg ggtgccctgt caagccatcc ccattcaaca
    11101 gccatattga gaatggcgcg aagtattgcc agatgcctcg tcttcgttcc tttgcttgca
    11161 agctttggtt tatactccgg caccggattg ccaagtcgca aacacctgtc ccggctcatc
    11221 tcccagttca ggcgatggcg gcggttttcc atcccgtcta ccgcctccat tattttttct
    11281 gttgttatgt cagagagaat ggcttctctg aagtgcaaca tccagaacga tataatgctc
    11341 ttgtcatcat caatggactt cttatccgat ttctcacgca gccaccgtat gcaggcttcc
    11401 ttgaatagct ttttcggtga ttccccgaga ttttttactc tccacgc
//
LOCUS       DAETPZ010000114        11435 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.114, whole genome shotgun sequence.
ACCESSION   DAETPZ010000114 DAETPZ010000000
VERSION     DAETPZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.114"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="K0W59_003514"
     CDS             complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="K0W59_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HBI3024401.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            321..1952
                     /gene="dcuS"
                     /locus_tag="K0W59_003515"
     CDS             321..1952
                     /gene="dcuS"
                     /locus_tag="K0W59_003515"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HBI3024402.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1949..2668
                     /gene="dcuR"
                     /locus_tag="K0W59_003516"
     CDS             1949..2668
                     /gene="dcuR"
                     /locus_tag="K0W59_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HBI3024403.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYDFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3239..4579
                     /gene="dcuB"
                     /locus_tag="K0W59_003517"
     CDS             3239..4579
                     /gene="dcuB"
                     /locus_tag="K0W59_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HBI3024404.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQL
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4657..6303
                     /gene="fumB"
                     /locus_tag="K0W59_003518"
     CDS             4657..6303
                     /gene="fumB"
                     /locus_tag="K0W59_003518"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBI3024405.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6426..7055
                     /locus_tag="K0W59_003519"
     CDS             6426..7055
                     /locus_tag="K0W59_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HBI3024406.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7194..8615)
                     /gene="melB"
                     /locus_tag="K0W59_003520"
     CDS             complement(7194..8615)
                     /gene="melB"
                     /locus_tag="K0W59_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HBI3024407.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8718..10073)
                     /gene="melA"
                     /locus_tag="K0W59_003521"
     CDS             complement(8718..10073)
                     /gene="melA"
                     /locus_tag="K0W59_003521"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBI3024408.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            10356..11264
                     /gene="melR"
                     /locus_tag="K0W59_003522"
     CDS             10356..11264
                     /gene="melR"
                     /locus_tag="K0W59_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HBI3024409.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
BASE COUNT         3003 a         2936 c         2763 g         2733 t
ORIGIN      
        1 gactttcgtt tgagattaaa taacttgccg ttgccacgta cgcaattgcc agaatgctga
       61 catatcgcag tgttgaaata gacatcgatc ttttcgccgg tataacagcg ataacccccg
      121 tccagtagcg cctgatccat gaccttccct ctctcatcag tatgattatc gctataagca
      181 tagccccgga atcttcatac agcactggca gtttccgttg ccagagtctc ttcccctata
      241 tagccaatca tttttactcc ctttgaatta cccgcctcat cagagataat gcttaagaaa
      301 tttgtcacac aaggaagctg atgagacatt cattgcccta ccacatatta cgcaaacgtc
      361 cgatgaaatt gagtaccact gtgatcttaa tggtcagcgc ggtactgttc tcggtgctat
      421 tggtggtgca tctgatttac ttctcgcaaa tcagtgatat gacgcgagac gggctggcca
      481 acaaggcact ggcagtggcg cgtaccctcg ccgactcgcc ggaaatccgt cagggcttgc
      541 agaaaaaacc gcaggagagt ggcatccagg ccatcgcgga agccgtgcgc aaacgcaacg
      601 atctgctgtt tattgtcgtt accgatatgc aaagtcttcg ctactcacat cctgaagccc
      661 agcgtattgg tcagccattt aaaggtgatg acatccttaa tgcgctgaat ggcgaagaaa
      721 atgtcgctat caatcgcggt tttctggcgc aggctttacg cgtatttacc cccatctacg
      781 atgaaaatca taaacaaatt ggcgtggtgg cgatcggcct tgagttaagc cgcgtgaccc
      841 aacagatcaa tgacagtcgc tggagcatta tctggtcggt attatttggc atgctggtcg
      901 gactgattgg cacctgcatt ctggttaagg tactgaaaaa aatccttttc ggcctggaac
      961 cctacgaaat ctccacgctg tttgagcaac gccaggccat gttgcagtct atcaaagaag
     1021 gcgtcgttgc cgtggacgat cgcggcgagg tcacgctgat caacgatgcc gcacaagaat
     1081 tgctgaatta ccgtaagtcg caggacgatg agaaactgtc gacgctcagc cactcatggt
     1141 cacaggtggt agatgtctcg gaagtgttac gcgacggtac gccgcgccgc gacgaagaga
     1201 ttacgattaa agaccggcta ttactgatca acaccgttcc ggtgcgcagt aatggcgtta
     1261 tcatcggtgc catttcaacc ttcagggaca aaactgaagt tcgtaaactg atgcagcgac
     1321 tcgacggtct ggtcaactat gctgacgcac ttcgtgaacg atcccacgaa tttatgaata
     1381 aattgcatgt gattctcgga ttattgcatc tgaagagtta taagcagttg gaagattaca
     1441 ttctcaaaac agccaataac tatcaggaag agattggctc tctgctgggt aagatcaaat
     1501 ctccggttat cgctggtttt ttaatcagca agattaaccg cgcgaccgat ttaggccata
     1561 cgctgatttt aaacagtgaa agccagctgc cagacagcgg cagtgaggac caggtcgcga
     1621 cgctgattac cacattggga aatctgatag aaaacgcgct ggaagcatta gggccggaac
     1681 ccggtggcga aattagcgta acattgcact accgtcacgg ctggctgcac tgcgaagtta
     1741 acgatgatgg accggggatc gcacccgaca aaatcgatca catttttgac aaaggtgtct
     1801 cgacaaaagg aagcgagcga ggcgtcggtt tagcacttgt caaacaacag gtagaaaatc
     1861 tcggcggcag catcgccgtg gaatcggaac cggggatttt cacacaattt tttgtccaga
     1921 taccctggga cggggagagg tcgaacagat gatcaatgta ttaattatcg atgacgacgc
     1981 aatggtcgcg gagctgaatc gccgatacgt agcacaaatc ccaggctttc aatgctgtgg
     2041 aacagcctcg acgctggaga aagccaaaga gattatcttc aatagcgatg cgcctatcga
     2101 cctgatattg ctcgatatct atatgcaaaa agagaacggg ctcgatttac tgcctgtcct
     2161 gcataacgcg cgttgcaaaa gtgatgtgat tgtcatctcc tccgcagccg atgcggcaac
     2221 cattaaagat tcgctgcatt acggtgtcgt ggattacctg atcaaaccct tccaggcttc
     2281 ccgctttgaa gaggcgctca ccggctggcg gcaaaagaaa atggcgctgg aaaaacatca
     2341 gtattatgac caggccgagc ttgatcagct aatccacggc agcagctcca acgaacagga
     2401 tcctcgtcgc ttgccaaaag gcttaacgcc gcagacgtta cgcacgctgt gccagtggat
     2461 tgacgcgcat caggactatg acttttcaac cgacgagctg gctaacgagg ttaacatctc
     2521 gcgtgtttcc tgccgaaaat acctcatctg gctggtcaac tgccacatct tgttcaccag
     2581 tatccattat ggcgtcacgg ggcgtccggt ttaccgttac cgtattcagg cagaacacta
     2641 ctcactgctg aaacaatatt gccaataacg gatcgctggt tatctgtaag taataattat
     2701 taccgtaatg caaaataccc ctggcacaag ttacatcgtt aatcagtaaa acttgtgcca
     2761 gatcaaatat aattatccct ccatcatcgt taaaaattaa tatctcttca gatgaacggt
     2821 gtttttaatt tcaaaacgat aacaaaagtt aattaactat tatgtcaccc gcattatgtg
     2881 tatttttacc cacaaatggg tagatcagat taatctataa acctaatgat atctgccctg
     2941 agaacaaaaa atagaccgat aaatatcaat aagataacag caaacaaaac attaacatct
     3001 gcgcagtaca aactataaac ccatcgccag agagtttttc tctctgaaaa agccgcttat
     3061 cacagtgcat aaatttgccg ctgctttaat cagccaatat tcactgtgag gtatttgcta
     3121 aagccggtaa cgaccaaacg gatatttagt caggctctga aaacagttca tacaaaacag
     3181 aacgtgactg tgatctattc agcaaaaatt taaataggat tatcgcgagg gttcacacat
     3241 gttatttact atccaactta tcataatact aatatgtctg ttttatggtg ccagaaaggg
     3301 tggtatcgcg ctgggtttat taggcggtat cggtctggtc attctggtct tcgtcttcca
     3361 ccttcagcta ggtaaaccac cagttgatgt catgctggtt atcattgcgg tggtggcggc
     3421 atcggcgacc ttgcaagctt cgggcggtct tgatgtcatg ctgcaaattg ccgagaagct
     3481 gctgcgccgc aacccgaaat atgtctcaat tgtcgcgccg tttgtgacct gtacactgac
     3541 cattctttgc ggtacgggtc atgtggttta caccattctg ccgatcatct acgacgtcgc
     3601 cattaagaac aacatccgtc cggaacgtcc gatggcggca agttctatcg gtgcacagat
     3661 ggggattatc gccagtccgg tgtcggttgc ggtcgtgtct ctggttgcga tgctgggtaa
     3721 tgtcaccttt gatggtcgcc atcttgagtt cctcgatctg ctggcaatca ccattccatc
     3781 gacgttaatc ggtatcctgg cgatcggtat cttcagctgg ttccgcggta aagatctgga
     3841 taaagacgaa gagttccaga aattcatctc cgtaccggaa aaccgtgagt atgtttacgg
     3901 tgataccgcg acgctgctgg ataaaaaact gccgaaaagc aactggctgg caatgtggat
     3961 tttcctcggg gcaatcgctg tagtcgccct tcttggtgct gattcggacc tgcgtccatc
     4021 cttcggcggc aaaccgctgt cgatggtact ggttattcag atgtttatgc tgctgaccgg
     4081 ggcgctgatt attatcctga ccaaaaccaa tcccgcgtct atctcaaaaa acgaagtctt
     4141 ccgttccggt atgatcgcca tcgtggcggt gtacggtatc gcatggatgg cagaaaccat
     4201 gttcggtgcg catatgtctg aaattcaggg cgtactgggt gaaatggtga aagagtatcc
     4261 gtgggcgtat gccattgttc tgctgctggt ttccaagttt gtaaactctc aggctgcggc
     4321 gctggcggcg attgttccgg tcgcgctggc gatcggcgtt gatccggcat acatcgtggc
     4381 ttcagcaccg gcttgctacg gttattacat cctgccgact tatccgagcg atctggcagc
     4441 gattcagttt gaccgttccg gcaccaccca catcggtcgc ttcgtcatca accacagttt
     4501 tattctgccg gggttgattg gtgtgagcgt atcgtgcgtc ttcggctgga tcttcgccgc
     4561 gatgtacggg ttcttataaa tgcactttgc gtgccgcccg tgactacgcg gcacgccatt
     4621 ttcgaataac aaatacagag ttacaggctg gaagctatgt caaacaaacc ctttatctac
     4681 caggcacctt tcccgatggg gaaagacaat accgaatact atctactcac ttccgattac
     4741 gttagcgttg ccgacttcga cggcgaaacc atcctgaaag tggaaccaga agccctgacc
     4801 ctgctggcgc agcaagcctt tcacgacgct tctttcatgc tccgtccggc acaccagaaa
     4861 caggttgcgg ctattcttca cgatccagaa gccagcgaaa acgacaagta cgtggcactg
     4921 caattcttaa gaaactccga aatcgccgcc aaaggcgtgc tgccgacctg ccaggatacc
     4981 ggcaccgcga tcatcgtcgg taaaaaaggc cagcgcgtgt ggaccggtgg cggtgatgaa
     5041 gaagcgctgt cgaaaggcgt ctataacacc tatatcgaag ataacctgcg ctactcgcaa
     5101 aacgcggcgc tggacatgta caaagaggtc aacaccggca ctaacctgcc tgcgcaaatc
     5161 gacctgtacg cggtagatgg cgatgagtac aaattccttt gcgttgcgaa aggcggcggc
     5221 tctgccaaca aaacgtatct ctaccaggaa accaaagccc tgctgactcc cggcaaactg
     5281 aaaaacttcc tcgtcgagaa aatgcgtacc ctcggtactg cagcctgccc gccgtaccat
     5341 atcgcgtttg tgattggcgg tacgtctgcg gaaaccaacc tgaaaaccgt caaattagca
     5401 agcgctcact attacgatga actgccgacg gaagggaacg aacatggtca ggcgttccgc
     5461 gatgtccagc tggaacagga actgctggaa gaggcccaga aactcggtct tggcgcgcag
     5521 tttggcggta aatacttcgc gcacgacatt cgcgttatcc gtctgccacg tcacggcgca
     5581 tcctgcccgg taggcatggg cgtctcctgc tccgctgacc gtaacattaa agcgaaaatc
     5641 aaccgcgaag gtatctggat cgaaaaactg gaacacaatc taggtcagta cattccacaa
     5701 gaactgcgtc aggccggtga aggcgaagcg gtgaaggtcg accttaaccg cccgatgaaa
     5761 gagatcctcg cccagctttc gcaatatccg gtttccactc gtttgtcgct cactggcacc
     5821 attattgtgg gtcgagatat tgcacacgcc aagctgaaag agctgattga cgccggtaaa
     5881 gaacttccgc agtacatcaa agatcacccg atctactacg cgggtccggc gaaaacccct
     5941 gccggttatc catcaggttc acttggccca accaccgcag gtcgtatgga ctcctatgtg
     6001 gatctgctgc aatcccacgg cggcagcatg atcatgctgg cgaaaggcaa ccgcagccag
     6061 caggttaccg acgcgtgtca taaacacggc ggcttctacc tcggtagcat cggcggtccg
     6121 gcggcggtac tggcgcagca gagcatcaag catctggaat gcgtcgctta tccggagctg
     6181 ggtatggaag ctatctggaa aattgaagta gaagatttcc cggcgtttat cctggtcgat
     6241 gacaaaggta acgacttctt ccagcaaatc gtcaacaaac agtgcgcgaa ctgcactaag
     6301 taacctcttc ggcccagcac ctggcagcat gctgccaggt gatccccctg ggccacctct
     6361 tttgcgattg taatttcatg cttgctggtg aatagtcagt attttccctg acttgcgaac
     6421 tcaccatgac ccgcacactc aagccgttaa ttcttaacac cggcgcactg gcgctaacgt
     6481 taatcctgat ttataccggc atttcggccc atgacaaact cacctggctg ctggaagtga
     6541 caccggtgat tatcgtcgtg ccgctactac ttgccaccgc cagacgttac ccgttaacgc
     6601 tgctgctcta tacgctcatc ttctttcacg ccatcatcct gatggtaggc gggcagtaca
     6661 cctacgcgaa agtccccgtt ggttttgagg tgcaggaatg gctggggttg agtcgtaatc
     6721 cgtatgacaa gctggggcac tttttccagg ggctggtgcc tgcactggtg gcacgagaaa
     6781 ttctcgttcg cgggatgtac gtgcgcggac gtaaaatggt ggcgtttctg gtctgttgcg
     6841 tggcgctggc gataagcgcg atgtatgaat taatcgagtg gtgggcggca ctggcgatgg
     6901 gccagggagc ggatgatttt ctcggtaccc agggcgacca gtgggacacg caatccgata
     6961 tgttctgcgc gctgcttggc gcattaacga cagtgatatt gctcgctcgc tttcattgcc
     7021 gccagctacg gcgctatggc ttgataaccg gataacaacg tgcactttgt tgatgccgga
     7081 tgcggcgtga attccttatc cggtctacaa aatttgcaaa tccaaagcat tacattaaga
     7141 cgtggcctga taaacatatc gcatcaggcc attttcgtta gctattgtcc acgtcaggct
     7201 ttcacatcac tcactgcacc gaccggaata tcagcatgaa caggctcggg cggtactttg
     7261 cgatatttat ccagcagatg aatctggatc ctgcgcagcg tgtcgccatt gaggcgatag
     7321 aaacggaagt agagaatcag cgttaccatg aaaaacagag tcggtagagc aatcataata
     7381 aactgcatac ctaatagggc ttgcgtagac tgttcaacat tcggtacata gccaatcatc
     7441 cctaacacaa ccgcaatgaa aaaagccgca aaggctgaac cgcccttcac caccatagtc
     7501 tgcacggagt aagcgatgct ttcacagcgt acgtgcagtt tatattcacc gtaatcaacg
     7561 gtatctgcca ccatgatgac ctgtaatacc cagaaaagcg ccgttcccac attcagcaaa
     7621 atacccgcaa tcacaatgag gacgacgttg tgatagctca ttaatgccat taacaggaga
     7681 acaccacagc ttaacaccgg aagaatagat gctccggccc ataaaatgcg tcgggataat
     7741 gatttaacta agcgtgggaa gaatactaac gtcaccaggt tagcagctcc cgcatacgac
     7801 agataatagg ggaacaaatc cgcatcaccg ataacatatg agaaataata gatagcaaat
     7861 ccggtaataa tgttgctggc tacattataa gcaagagcca tacccaagag gcatgaaagc
     7921 tgatcgtttt tataaataag cgcaacgatg gctttaagtg tcagatggct tccttcagca
     7981 gacgattgat tgtctgacga aaagacttca tgcacattgc gcagagtgat gatggttgaa
     8041 acaataaaaa aggcgatcaa taccagagtg aacatctgaa agccaaatcc ccgatcgcca
     8101 ccgccgacat aattaacaaa tggtagcgtc acacctgccg taacaaagcc tgccagactg
     8161 gcaaaaaaac gcggataagg aaccagttgt tcgcgttcac gtttatcgag cgtgatggtt
     8221 ggaaccagcg accagaaggg aatatccata atggtgtaag tcatgcccca gaggatgtag
     8281 gtcacgcaaa caaagacaat ctgagtagta ccttcaaaca gatgcgcact aaagaggaga
     8341 aataagatta cagagtttgc caacgtaccg atcaggatcc agggtttgaa cttaccccat
     8401 cgcgatcgcg tagcatttac aatccatccc ataatcggat cgttaatagc atcccagatc
     8461 ctcgccacca gaaacaaagt accgaccaaa cccacagaca gcccgacgac atcggtgtag
     8521 taatacatga ggtacatata cacaatgccg atcgcaaaat ccttcccgaa cgctccaaat
     8581 ccataactga gttttgtagt cattgaaatg ctcatagggt atcgggtcgc tgttaccaac
     8641 gcccgccttc tgttattggt atcgtcagag gtgtgcgccg gaataaatcc cccggcgcag
     8701 tagcgtttag tcgcgtttta acggtgcaac cagcctggca gccagtcgcc gtgggcggca
     8761 atcaggtcgt caacaagagc atatatttcg tcaatgccca gcacggcggc agtatgcggg
     8821 tccatcatcg cggcgtggta aacacggtcg cgattttccg taagaatagc ttcggtcagc
     8881 agcgtctgta cgttgatgtt ggtttgcatc agggcggcca gatgcgaagg tagcgtaccg
     8941 actttggtcg gctgaatgcc attagcatca accagacagg ctacttccac gcaacatcct
     9001 tgtggcaggt tatcaatcaa accatcgtta cggacgttgc cgtaaatcac actcggctcg
     9061 ccagtccaga tagcgttcat gattgtgctg gcatattccc gtgacggttt aatatcaatc
     9121 cgggaggcgt ttttatactc ctccagctct ttatgccagt tcgccagctg ctcgacgcag
     9181 cgtttcgggt actcatccag cggtacttta taacgctcaa tcaaatcctc acgacctagc
     9241 ttaataaacc acggcgtata ttcggcaaaa tgttctgacg attccgtgac gaagtagccc
     9301 agctttttga acatttcgta gcgcacaata ttctggcagc gagtattacc atgaatattt
     9361 ggcttcggtg cctgccctgc ttcataagcc gccagcagtt ccgggtagag attcacgtaa
     9421 ctgccgtcgg cggttttgcg ctccagctcc aggtaaaacg ccatatggtt gatacctgcg
     9481 caacggtaac gcagcgtagc cgggtcgata ttgaggtcac gcgccagctc ttccgccgtt
     9541 ccctgcaccg aatggcacag cccgacctgt ttgatatacg gatagcgggc atacatcgcc
     9601 caggtattca tcgccattgg gttaacatag ttgagcatgg tggcatcggg gcagacttcc
     9661 gtcatgttct cgcaaatttg ccacagatgc ggaatggtac gtagcgcgcg cataataccg
     9721 cccggcccca acgtatcggc aatggtttgt tccagaccat gccgcttaca gacctcgaaa
     9781 tcagtcaccg tgcaaggttc ataaccgcca atctgaaatg ccaccacgac aaaatcggca
     9841 tcctctaagg cttctttctg ttgggtgtgg caggtgattt tgccgctggc ccctgctgaa
     9901 tccatcagct tacgcaccac aatatgcgac tcttccaggc gggtggggtc aatgtccatc
     9961 agggcaatat gcgccgtttt cagcgcctcg cgatggaaca catcaccaag aatattttta
    10021 acgaaaatcg tcgaaccagc gccgataaat gtaattttgg gtgcagacat catgcagatc
    10081 tcctggcttg cttgaataac ttcatcatgg caggcgaatc tgcagaaaac ctccggcttc
    10141 caggttgagc attcccataa actcagatcc tgcgtgaagc agcagtaaat ctgagtttat
    10201 gggaataaat tacctgaaaa gtagagtttt cggcctcatg atgctcggac agtcccctcg
    10261 cccctctggg aagagggtga aaaccgtgct cccactcgca gtcatcctcc ctcactcctg
    10321 ccataattct gatattctag gaaagagagc catccatgaa tacagatacg tttatgtgca
    10381 gcagcgacga aaaacagacc cgcagcccgc tgtcgctgta ttcggaatac cagcggatgg
    10441 agattgagtt tcgcgcaccg catatcatgc ccaccagcca ctggcatggt caggtcgaag
    10501 tgaatgtgcc tttcgatggc gatgtggaat acctgatcaa caatgaaaaa gtgaatatca
    10561 atcagggtca tatcacgctg ttctgggcct gtacaccgca ccaactaaca gataccggaa
    10621 cctgtcagag catggcgatt tttaatctgc cgatgcatct gtttctctcc tggccgctgg
    10681 ataaagacct gattaaccac gtcactcacg gcatggtgat caaatcactt gccacacagc
    10741 aacttagccc gtttgaagta cgccgctggc agcaggaatt aaacagtccg aatgagcaaa
    10801 ttcgccagct cgccattgat gaaattggcc tgatgctcaa gcgatttagc ctctctggct
    10861 gggaaccgat tctggtcaat aaaacctcgc gcacacacaa aaacagcgtc tcgcgccatg
    10921 cgcaatttta tgttagccag atgctgggct ttattgccga aaactatgat caggcgctga
    10981 ccatcaacga tgtggctgag cacgtcaaac ttaacgccaa ctatgcaatg gggatattcc
    11041 agcgggttat gcaattgacg atgaaacagt acattaccgc gatgcgcatc aaccacgttc
    11101 gcgcgttact gagcgatacc gataaaagta ttctcgatat tgccctgacg gcaggctttc
    11161 gttcaagtag tcgtttttac agcacgttcg gcaaatatgt cggcatgtcg ccgcaacaat
    11221 accgcaaact tagccaacag cgccgccaga cgtttcccgg ctaaagacga tatcagtatc
    11281 agccaaaaaa atagtttagc cgtcgataat aagcagtgaa aataatttaa aataatcaca
    11341 taacgtattg tttataaaac taaatatttc aaaaatgttt gtttttcacg cgctttacag
    11401 cccgaaaagg ccggaagata cttgcccgca acgaa
//
LOCUS       DAETPZ010000115        11184 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.115, whole genome shotgun sequence.
ACCESSION   DAETPZ010000115 DAETPZ010000000
VERSION     DAETPZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.115"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            215..2920
                     /gene="malT"
                     /locus_tag="K0W59_003523"
     CDS             215..2920
                     /gene="malT"
                     /locus_tag="K0W59_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HBI3024410.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWNLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALVQQTEQIARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(2963..3840)
                     /gene="rtcA"
                     /locus_tag="K0W59_003524"
                     /pseudo
     CDS             complement(2963..3840)
                     /gene="rtcA"
                     /locus_tag="K0W59_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
     gene            complement(3844..5070)
                     /gene="rtcB"
                     /locus_tag="K0W59_003525"
     CDS             complement(3844..5070)
                     /gene="rtcB"
                     /locus_tag="K0W59_003525"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HBI3024411.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            5259..6857
                     /gene="rtcR"
                     /locus_tag="K0W59_003526"
     CDS             5259..6857
                     /gene="rtcR"
                     /locus_tag="K0W59_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HBI3024412.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKAAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(6839..7597)
                     /locus_tag="K0W59_003527"
     CDS             complement(6839..7597)
                     /locus_tag="K0W59_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HBI3024413.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(7614..8444)
                     /gene="glpG"
                     /locus_tag="K0W59_003528"
     CDS             complement(7614..8444)
                     /gene="glpG"
                     /locus_tag="K0W59_003528"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HBI3024414.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(8489..8815)
                     /gene="glpE"
                     /locus_tag="K0W59_003529"
     CDS             complement(8489..8815)
                     /gene="glpE"
                     /locus_tag="K0W59_003529"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HBI3024415.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            9005..10510
                     /gene="glpD"
                     /locus_tag="K0W59_003530"
     CDS             9005..10510
                     /gene="glpD"
                     /locus_tag="K0W59_003530"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HBI3024416.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSIGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(10779..11009)
                     /locus_tag="K0W59_003531"
                     /pseudo
     CDS             complement(10779..11009)
                     /locus_tag="K0W59_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         2694 a         3062 c         2865 g         2563 t
ORIGIN      
        1 gatgaacata agggaaacca gtattcacgc tggatcagcg tcgttttagg tgagttgtta
       61 ataaagattt ggaattgtga cacagtgcaa attcagacac ataaaaaaac gtcatcgctt
      121 gcattagaaa ggtttctggc cgaccttata accattaatt acgaagcgca aaaaaaataa
      181 tatttcctca ttttccacag tgaagtgatt aactatgctg attccgtcaa aactaagtcg
      241 tccggttcga ctcgaccata ccgtggttcg tgagcgcctg ctggctaaac tttccggcgc
      301 gaacaacttc cggctggcgc tgatcacgag tcctgcgggc tacggaaaga ccaccctcat
      361 ttcccagtgg gcggcaggca aaaacgatat cggctggtac tcgctggatg aaggtgataa
      421 ccagcaagag cgtttcgcca gctatctcat tgccgccgtg cagcaggcaa ccaacggtca
      481 ctgtgcgata tgtgagacga tggcgcaaaa acggcaatat gccagcctga cgtcactctt
      541 cgcccagctt ttcattgagc tggcggaatg gcatagccca ctttatctgg tcatcgatga
      601 ctatcatctg atcactaatc cagtgatcca cgagtcaatg cgcttcttta ttcgccatca
      661 accagaaaat ctcaccctgg tggtgttgtc acgcaacctt ccgcaactgg gcattgccaa
      721 tctgcgtgtt cgtgaccaac tgctggaaat tggtagtcag caactggcat ttacccatca
      781 ggaagcgaag cagttttttg attgccgtct gtcatcgccg attgaagccg cagaaagcag
      841 tcggatttgc gatgacgttt ccggttgggc gacggcacta cagctaatcg ccctctccgc
      901 ccggcagaat acccactcag cccataagtc ggcacgccgc ctggcgggaa tcaatgccag
      961 ccatctttcg gattatctgg tcgatgaggt tttggataac gtcgatctcg caacgcgcca
     1021 ttttctgttg aaaagcgcca ttttgcgctc aatgaacgat gccctcatca cccgtgtgac
     1081 cggcgaagaa aacgggcaaa tgcgtctcga agagattgag cgtcaggggc tgtttttaca
     1141 gcggatggat gataccggcg agtggttctg ctatcacccg ctgtttggca acttcctgcg
     1201 ccagcgctgc cagtgggaac tggcggcgga gctgccggaa atccaccgtg ccgccgcaga
     1261 aagctggatg gcccagggat ttcccagcga agcgattcat catgcgctgg cggcaggcga
     1321 tgcgctgatg ctgcgcgata ttctgcttaa tcacgcctgg aatttgttca accatagcga
     1381 actgtcgctg ctggaagagt cgcttaaggc cctgccgtgg gacagtttgc tggaaaatcc
     1441 gcagttagtg ttattgcagg cgtggctaat gcaaagccaa catcgctatg gcgaagttaa
     1501 caccctgcta gcccgtgctg aacatgaaat caaggacatc agagaaggca ccatgcacgc
     1561 agaatttaac gctctgcgcg cccaggtggc gattaacgat ggtaatccgg atgaagcgga
     1621 acggctggca aaactggcac tggaagagct gccgccgggc tggttctata gccgcattgt
     1681 ggcaacctcg gtgctgggtg aagtgctgca ctgcaaaggc gaattgaccc gctcactggc
     1741 gctagtgcag caaaccgaac agatagcacg ccagcacgac gtctggcact acgccttatg
     1801 gagtttaatt cagcaaagtg aaattctgtt tgcccaaggg ttcctgcaaa ccgcgtggga
     1861 aacgcaggaa aaagcgttcc agctgatcaa cgagcagcat ctggaacagc tgccaatgca
     1921 tgagtttctg gtgcgcatcc gagcacaact gttatgggcc tgggcacggc tggatgaagc
     1981 cgaagcgtcg gcgcgtagcg ggattgaagt cttgtcgtct tatcagccac agcaacagct
     2041 tcagtgcctg gcaatgttga ttcaatgctc gctggcccgt ggtgatttag ataacgcccg
     2101 tagccagttg aaccgtctgg aaaacctgct ggggaatggc aaatatcaca gcgactggat
     2161 ctctaacgcc aacaaagtcc gggtgattta ctggcaaatg accggcgata aagccgccgc
     2221 tgccaactgg ttgcgtcata cggctaaacc ggagtttgcg aacaaccact tcctgcaagg
     2281 tcaatggcgc aacattgccc gcgcacaaat cttgctgggc gagtttgaac ctgcggaaat
     2341 tgttctcgaa gaactcaatg aaaatgcccg cagtctgcgg ttgatgagcg atctcaaccg
     2401 taacctgttg ctgcttaatc aactgtactg gcaggccgga cgtaaaagtg acgcccagcg
     2461 cgtgttgctg gacgcattaa aactggcgaa tcgcaccgga tttatcagtc attttgtcat
     2521 cgaaggcgaa gcgatggcgc aacaactgcg ccagctgatt caacttaata cgctgccgga
     2581 actggaacag catcgcgcgc agcgtattct gcgagaaatc aatcaacatc atcggcataa
     2641 attcgcccat ttcgatgaga atttcgttga acgtctgcta aatcatccgg aagtgccaga
     2701 actaatccgc accagcccgt tgacccaacg tgaatggcag gtcctggggc tgatctactc
     2761 cggttacagc aatgaacaaa ttgcgggcga actggaagtg gcggcaacca ccatcaagac
     2821 gcatatccgc aatctgtacc aaaaactcgg cgtcgcccac cgtcaggctg ccgtgcagca
     2881 cgcgcagaaa ctgctgaaga tgatgggtta tggggtgtga gtttagccgg ataatgcatt
     2941 ggatccggtt tacatcttta catcattcaa tactcacccg cgttacgcca tctgtttcta
     3001 tcaaaccaaa ccgcaccggc aagaaacgct ccaccaccgc gatattggtc agcagatggc
     3061 atgaaggatg ggcgaccgta aattctcccg cgcccgccag cgccatcggc agtaccagtt
     3121 ggtcggcgag atattccccc accgccgctg ggcttgccag gtagcgtttc acctctttca
     3181 ctaactgcgc ggctaccacc tcggcactga cgcgcttttc accgacgacg aaaaagcgtt
     3241 cagtgatatt ttcactttcg acttccagcg agacggtatt acccggcccc tggtcgcgcg
     3301 gcaggttatg aatattctgt tcatgcaggg aaaaaatccc cgccagtgta gcgatttcac
     3361 gctcagcaac atggcgcggc acaccagcta atagaacttc tccacgcatc tgcacaatgt
     3421 tcccgcgctc gccaagttgc aaggtgctaa acgatgccac gggcgagact tccgttgcca
     3481 ccacaccgcc tccggcagga taaaaaccgt gacgtaacag cgtggtttgc tgatgaattc
     3541 ctattttcgc cagcagcggc tccagcaccc ggcggataaa atccgcaggc ggcgccgacg
     3601 ggttatcggt gccgccgctc acttcaacac gcgaaggtcc atcggcaaac cacagcgcgg
     3661 gcagcaccgt ttgcagcacc agcgtacaac ttccggcgct accgatagca aagcggtaat
     3721 cgccgccgcg cacggtgcca ggccggaaga ccagacgctg cgatcccagc tcagcgccga
     3781 gcgcaggatc tgcccgccgc cttcgccctg tgcgccatcc agcgcaatca tccttttcat
     3841 catttatcct tttacgcaca ccacctgacg cagggtgtag ataacttcca ccagatcgct
     3901 ttgtgccgcc atcaccgcat caatatcttt atacgccatc gggatttcgt cgatcacttc
     3961 ggcatcttta cggcattcca catgcgcggt ggcacgaatt tggtcttcca cgctgaacag
     4021 ttttttcgct ttagttcggc tcattacccg cccggcaccg tggctgcacg aacagaacga
     4081 ctcttcattt cccagcccac ggacgataaa gctttttgct cccatcgaac cgggaataat
     4141 tccatattga ccagcacgcg cagacaccgc acctttacgc gtcacgtaga tctcttcacc
     4201 aaagtgctgt tctttttgca catagttgtg gtgacagttg atctcttcca tcgccagcgt
     4261 ttgtggctgt ctgaccgttt tctgcgtaat gctctgcaac gccgttacca cattttccat
     4321 catcgcatcg cggttaaggc tggcgaaaag ctgcgcccag gccacggctc ttaggtaatc
     4381 atcaaagtat tccgtacctt ccataaagta cgccagatca cgcgacggca acgtctcaag
     4441 ttggtcctgc atctcttttt gcgccagatc gataaagtaa gtcccgatgg cgttaccaat
     4501 tccacgtgaa ccggagtgca gcataatcca cacctgctcc gactcatcaa ggcagatttc
     4561 aataaagtgg ttacccgttc ccagcgttcc caggtgttta tagttgttag tgttaaggaa
     4621 acgcggatat ttttgcgtta accactgata accggcctcc agctcagccc atttcgcatc
     4681 gacgttaaaa ggcgggtttt cccatgcgcc tttatcacgt ttacaacgcc cggtagtacg
     4741 cccgtgtggt acggccgttt caatcgcttg acgcagctcc gccaggtttt caggcaggtc
     4801 tgccgccgtt aacgcggtac gcagcgcgtt cattccacag ccaatatcca cgcccaccgc
     4861 cgccggaata atcgcccctt tggtcgggat cacgctacca atggtggaac ctttacccag
     4921 gtgtacatca ggcattaccg caatatgttt gaaaataaac ggcatcttcg ccgtattaat
     4981 aagttgctga cgcgcatcgg cttctaccgg cacgcctttg gtccacattt ttaccggggc
     5041 attttcagtg gtcagtaatt cgtaattcat tttgtttttc tcttttcgtt gtttgcggtc
     5101 ctgataattg caaccgtcgt gccagaaaat taaataagca gattaatttt ttaatttatt
     5161 gtttctaaaa agattatatc tttatgtccg taaacggaga tttcccgcaa agccgattta
     5221 ccgataatga aatatcgcct tttataagga tatctaagat gcgtaaaaca gtggcttttg
     5281 gctttgtcgg taccgtgctg gattatgccg gacgcggcag tcagcgctgg tcaaaatggc
     5341 gtccgtcact ctgtatatgc cagcaagaat cgttggtcat cgatcgactg gaattattgc
     5401 acgatgcccg ctcgcgctcg ctatttgaaa cgcttaaacg cgatatcgcc agcgtttcgc
     5461 cagaaacaga agtggtgggc gttgagattg aactgcataa cccgtgggat ttcgaagagg
     5521 tttacgcctg cctgcatgat ttcgcccgtg gttacgagtt tcagccagaa aaagaagact
     5581 atttaattca catcaccacc ggcacccacg tcgcgcagat ttgctggttt ctgctggcgg
     5641 aagcgcgtta tctacccgcc cggttgatac aatcttcaac tccgcgcaaa aaagaacagc
     5701 cgcgcggccc aggtgaagtg acgattatcg atctcgattt aagccgttat aacgccatcg
     5761 ccagccgctt tgccgaggaa cggcagcaaa cgctcgattt cctcaaatcc ggtattgcca
     5821 cgcgtaaccc ccacttcaac cgcatgattg agcagatcga aaaagtggcg attaaatccc
     5881 gcgcgccgat tctgcttaac ggcccgaccg gcgcaggtaa gtcatttctg gcgcgacgca
     5941 tcttcgagtt aaaacaggca cggcatcagt ttagcggcgc gtttgtggaa gtgaactgcg
     6001 ccaccctgcg cggcgatacc gcgatgtcga cgctgtttgg tcatgtgaaa gccgcgttta
     6061 ccggggcgcg ggaatctcgt gaaggattat tacgcagcgc caacggcgga atgttgtttc
     6121 ttgatgagat tggcgaactg ggcgcagacg aacaggcaat gctgctgaaa gctattgaag
     6181 agaaaacctt ttacccgttt ggcagcgatc gccaggtgag cagtgatttt cagcttatcg
     6241 ccggaacggt gcgcgatttg cgccagctgg ttgccgaagg caaattccgc gaagatctgt
     6301 acgcgcggat caatctctgg accttcaccc tgccgggtct acgccagcgc caggaagata
     6361 ttgaaccgaa cctggattat gaagtggagc gccacgccac actcactggt gacagcgtgc
     6421 gttttaacac cgaagcgcgg cgtgcctggc tggcctttgc aacatctccc caggctacat
     6481 ggcgcggtaa ctttcgcgaa ctttctgcca gcgtcacgcg gatggccacc tttgccacta
     6541 gcggacgcat cactctggac gtggtggaag atgagataaa ccgtctgcgc tataactggc
     6601 aggagagccg cccctccgcg cttacggcgt tgctgggcgc agaggcagaa aacatcgatc
     6661 tcttcgaccg tatgcaactg gaacacgtta tcgctatctg ccgccaggca aagtcgcttt
     6721 ctgctgccgg acgacagctt tttgatgttt cgcgccaggg caaagccagc gtcaatgacg
     6781 cggatcggct acgcaaatac ctggcgcgtt ttggtctgac gtgggaggcc gtgcaggatc
     6841 agcacagctc cagttgaata tggtggtccg tcagcacctg catcacgctt actggcggcg
     6901 gggcgtcggt gtagacggca tcaaccatgc tgatgctccc catattgacc atcgcattac
     6961 ggccaaattt tgaatggtca accaccagca taacgtggcg cgagttctca ataatggcgc
     7021 gcttggtacg aacttcgtgg taatcgaact ccagcagcga gccgtcgctg tcgatgccgc
     7081 tgatccccag aatgccgaaa tcaaggcgga actgggagat aaaatcgagc gtcgcttcgc
     7141 caatgatccc gccatcgcgg ctacgtaatt cgccaccggc gagaataatg cggaaatctt
     7201 cttttaccat caacgtgtta gcaacgttga gattgttggt gacaatgcgc aaattgctgt
     7261 gattgagcag cgcgtgcgcc accgcttccg gcgtggtgcc gatatcgata aacagcgtcg
     7321 agccattggg gatttgctcc gccactttgc gggcgatgcg ctctttttct tcggtctggg
     7381 tggctttacg atcgtgccac ggcgtgttaa ccgaactgga aggcagcgcc gcgccgccat
     7441 gatggcgcag gatcaggttt tgctctgcca gctcgttgag gtcgcggcga atagtctgcg
     7501 ggctgacgga gaagtgctcc accagttcct cggtactgac ataaccctgc tgtttaacca
     7561 gttcgataat accgttgtga cgttgtgttt gtttcattta taaatccctg gaattatttt
     7621 cgttttcgcg cattgagcga atcaacaaaa gccatcgcta aacccacggc taatccggcg
     7681 atgtgtgctc cgttcgccat cgacatccca aacaaatcaa accatccggc gacaatccag
     7741 atcagcgcaa agataattaa cccacgttgc aggtaaatgc cactttgcgg atcgcgttcg
     7801 ccacgtagcc agacgtagcc catcagcgca tacaccacgc cagaaagccc gccaaaccac
     7861 ggcccgctga atttttgctg cacatagccg cttaacaggg cgctgataag cgtaatgaca
     7921 attagcttac cgctaccgag gcgtttttcc accgcgccgc cgagatacca ccaccaaagc
     7981 aggttaaaga ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa gtaacgccag
     8041 aactcaaatt tcagtgttgg atcgaatggc caggccagcc ataacatcac ttcctgatcg
     8101 ccgagaattt gcatggcaat aaacaccacc acgcaggcga tcatcatcac ccaggtcacc
     8161 ggacctgcgc gttcacgcaa ggcagcaaag aaaggataac ggcgataatg caggccactg
     8221 ccggtatggc ctgccagcca gctggccgcc agataacgcg gatctgccgg gttttcgaga
     8281 aaacgcgcca gctccgcccg cacgcgctcg gcctgggact catccgccag ccagacatcg
     8341 ctttggttat gttgttgaat cgtgaggata acaccctgcg tcgccatgta atcaacaaac
     8401 gcctgcgcca cgcgggggtt agcaaaagag gtaatcatca acatcgttgc tgtcgcttat
     8461 tccacacaaa aggggacagt ataaagcgtt acgcgccgta cgccacctct gcgggaaact
     8521 gtcgctgcca ggcttcaaag ccgccgtcaa tgctatagac cacatcgtag ccctgttgca
     8581 gcagatactg cgccgcccct ttgctgctat tgccgtgata acacatcacc atcaccggag
     8641 tgtcaaagtc gttatcacgc ataaacgcgc ccagcgtgtc gttggttaaa tggaaagcct
     8701 gcaccgcatg ccccatagcg aaactctgtg gatcgcgaat atcgaccagc accgcctctt
     8761 tttcctgcaa cttctggtgc gcgtcggcaa cgttaataca ttcgaactga tccatgcgtc
     8821 tctctttctt tacaaacaag tgggcaaatt taccgcacag tttacgtcga agcggcagat
     8881 aaacgccata atgttataca tatcactcta aaatgttttt tcaatgttac ccaaagcgcg
     8941 attctttgct aatatgttcg ataacgaaca tttatgagct ttaacgaaag tgaatgaggg
     9001 cagcatggaa accaaagatc tgattgtgat aggtggcggc atcaatggtg ctggtatcgc
     9061 ggcagacgcc gctggacgcg gtttatccgt gctgatgctg gaggcgcagg atctcgcttg
     9121 cgcgacctct tccgccagtt caaaactcat tcacggtggc ctgcgctacc ttgagcacta
     9181 tgaattccgc ctggtcagcg aggcgctggc tgaacgtgaa gtgctgctga aaatggctcc
     9241 gcatatcgcc ttcccgatgc gttttcgcct gccacatcgt ccgcatctgc gcccggcgtg
     9301 gatgattcgc attggtctgt ttatgtacga tcatctgggt aaacgcacca gcctgccggg
     9361 atcaattggt ttgcgttttg gcgcaaattc agtgttaaaa ccggaaatta agcgcggatt
     9421 cgaatattct gactgttggg tagacgacgc ccgtctggta ctcgccaacg cccagatggt
     9481 ggtgcgtaaa ggcggcgaag tgcttactcg gactcgcgcc acctctgctc gccgcgaaaa
     9541 cggcctgtgg attgtggaag cggaagatat cgataccggc aaaaaatata gctggcaagc
     9601 gcgcggcctg gttaacgcca ccggcccgtg ggtgaaacag ttcttcgacg aggggatgca
     9661 tctgccttcg ccttatggca ttcgcctgat caaaggcagc catattgtgg tgccgcgcgt
     9721 gcatacccaa aagcaggcct acattctgca aaacgaagat aaacgcattg tgttcgtgat
     9781 cccgtggatg gacgagtttt ccatcatcgg caccaccgat gtggaataca aaggtgatcc
     9841 gaaagcagtg aagattgaag agagtgaaat caattacctg ctgaaagtgt ataacgcgca
     9901 ctttaaaaag cagctaagcc gtgacgatat cgtctggacc tactccggtg tgcgtccgct
     9961 gtgtgatgat gagtccgact cgccgcaagc cattacccgt gattacaccc ttgatatcca
    10021 tgatgaaaac ggcaaagcac cgctgctgtc ggtattcggc ggtaagctga ccacctaccg
    10081 aaaactggca gaacatgcgc tggaaaaact gacgccgtat tatcagggta ttggcccggc
    10141 gtggacgaaa gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgcg acgattacgc
    10201 cgctcgcctg cgccgccgtt acccgttcct gactgaatcg ctggcacgtc actacgcgcg
    10261 cacttacggc agcaacagcg agctgctgct cggcaatgcg ggaacggtaa gcgatctcgg
    10321 ggaagatttc ggtcatgagt tctacgaagc ggaactgaaa tacctggtgg atcacgaatg
    10381 ggtccgccgc accgacgatg ccctgtggcg tcgcacaaaa caaggcatgt ggctgaatgc
    10441 ggatcaacaa tctcgtgtga gtcagtggct ggtggaatat acgcagcaga agttgtcgct
    10501 ggcgtcgtaa attaacgtaa ggtgatcagg tcagatttca atctggcctg agactgatga
    10561 caaacacaaa actgcctgat gcgctacgct tatcaggcct acgtggttta tgcaatatat
    10621 tgaatttgca tggtcttgta ggccagataa gacgttcacg tcgcatccgg catgaacaaa
    10681 gcgcactttg tcaaaaatct aacctacttt ttaattcagg gaattaccgc aaagcccacg
    10741 ccatcatacc caacgtaaca aaactcaggc acgttcccca ctcgccccga gaaaaatagc
    10801 attaatgcgc ccagcgtcag cataaaaata ttgagcagaa ggtaaattgt tggcgtaaag
    10861 agtaaattgc aaacctcagg aatagcggca agcccagccc ccagcatggc aataaccgct
    10921 gcaaaaaatt gcgtcagtat tgggatgccg aaaaaagtca ttaccagcga ggtcaaaatc
    10981 cattttctat tttgcattat tctttccatt ctttctgaat ggcgaaatta tactcccctt
    11041 gccccttctg gacacttttc cgaaatgatg gcgaaaaaaa cgggaccctt tggcctaatg
    11101 ccagtcagtt aagcaactga ctggctcttt ttcggggctg tggggtattt ccagggcctc
    11161 tcctttacca ctctcgggaa ggcc
//
LOCUS       DAETPZ010000116        10815 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.117, whole genome shotgun sequence.
ACCESSION   DAETPZ010000116 DAETPZ010000000
VERSION     DAETPZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10815
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.117"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            93..1178
                     /locus_tag="K0W59_003532"
     CDS             93..1178
                     /locus_tag="K0W59_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBI3024417.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            1249..1857
                     /locus_tag="K0W59_003533"
     CDS             1249..1857
                     /locus_tag="K0W59_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HBI3024418.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1955..3346
                     /gene="selA"
                     /locus_tag="K0W59_003534"
     CDS             1955..3346
                     /gene="selA"
                     /locus_tag="K0W59_003534"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBI3024419.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            3343..5187
                     /gene="selB"
                     /locus_tag="K0W59_003535"
     CDS             3343..5187
                     /gene="selB"
                     /locus_tag="K0W59_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBI3024420.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPER"
     gene            5377..6528
                     /gene="yiaY"
                     /locus_tag="K0W59_003536"
     CDS             5377..6528
                     /gene="yiaY"
                     /locus_tag="K0W59_003536"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HBI3024421.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            6693..8231
                     /gene="aldB"
                     /locus_tag="K0W59_003537"
     CDS             6693..8231
                     /gene="aldB"
                     /locus_tag="K0W59_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HBI3024422.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(8450..8605)
                     /locus_tag="K0W59_003538"
     CDS             complement(8450..8605)
                     /locus_tag="K0W59_003538"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024423.1"
                     /translation="MAKLMNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRP
                     LTIETRD"
     gene            8776..9099
                     /locus_tag="K0W59_003539"
     CDS             8776..9099
                     /locus_tag="K0W59_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBI3024424.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLISKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            9105..10241
                     /locus_tag="K0W59_003540"
     CDS             9105..10241
                     /locus_tag="K0W59_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBI3024425.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQVR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(10238..>10815)
                     /locus_tag="K0W59_003541"
     CDS             complement(10238..>10815)
                     /locus_tag="K0W59_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3024426.1"
                     /translation="ERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYAS
                     QRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNT
                     QSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRV
                     QYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         2577 a         2659 c         2977 g         2602 t
ORIGIN      
        1 atcgccgcac tttctgaccg aatttcccgg ctggagcatc aactcgccgc cgagaaaaag
       61 actgactatt ccacgttccc ggagatctaa ttatggatct attgattgtt ttaacttacg
      121 tggcgctggc gacggcggcg ctgggaggcg tgtttctggt gagtggtttg attttgttga
      181 tgaactacaa ccacccttac acttttaccg cgcaaaaggc agtgatagcg atccctatca
      241 cgccacaggt gacgggaatt gttactgaag tcactgacaa gaataatcag cttattcaaa
      301 agggcgaggt gctttttaag ctcgacccgg ttcgttacca ggcgcgagtt gacagacttc
      361 aggctgacct gatgacggcg acgcataata taaagacgct gcgtgcgcag ctcactgaag
      421 cgcaggccaa caccacccag gtttcagcgg agcgcgaccg tctgtttaaa aattatcaac
      481 gttacctgaa aggcagccag gcggcggtga atccgttctc ggaacgtgac atcgacgatg
      541 cgcggcaaaa tttcctcgcg caggatgcgt tagtgaaagg ctcggtggcg gagcaggcgc
      601 agatccagag tcagctcgac agtatggtca acggcgagca atcgcagatt gtgagcttaa
      661 gagcgcaact tactgaagca aaatataatc ttgagcagac tgtcattcgc gcaccaagca
      721 atggctacgt cactcaggta ctgatccgcc caggtacata cgcagctgcc ttgccgctgc
      781 gtccggtgat ggtcttcatc cccgagcaaa aacggcaaat tgtcgcccaa tttcggcaaa
      841 actcgctgtt acgtctgaaa cctggcgatg atgcggaagt ggtgtttaac gcgctacctg
      901 ggcaggtgtt ccacggcaaa ttgactagta ttttacctgt cgtgccaggc ggttcttatc
      961 aggcgcaggg ggtattgcaa tcattaacgg tcgttcccgg cacggacggt gtgctgggaa
     1021 ccattgaact ggaccctaac gatgatatcg atgccttacc cgacggcatc tacgcccagg
     1081 tggcggttta ctccgaccat ttcagccatg tttcggtgat gcggaaagtg ctgctaagaa
     1141 tgaccagttg gatgcattat ctttatttgg atcattgaga cttatatcac ctgtgttttg
     1201 cgtgagcctg agccatactg atatttttcc tcgcacaaag gactcggcat gaaactcgtc
     1261 ggtagctaca ccagcccgtt tgtacgcaaa ctttctattc tgttgttaga aaagggcata
     1321 actttcgaat ttattaatga actgccctat aacgcggata acggcgtggc gcaatttaac
     1381 ccgttaggga aagtgccggt gctggtgacc gaagagggcg aatgctggtt tgattcgccg
     1441 atcatcgctg aatatattga gttaatgaat gtcgctccgg cgatgttgcc gcgcgatccg
     1501 ctggagtcgt tgcgggtgcg caaaattgag gcgctggcgg atggcattat ggatgccggg
     1561 ctggtatcgg tgcgtgaaca ggcgcgtccg gcggcgcagc agtctgaaga tgaattgtta
     1621 cgccagcggg agaaaatcaa ccgcagtctg gatgtgctgg aaggatatct ggtcgatggc
     1681 acactcaaaa ccgatacggt caatctggcg actatcgcca ttgcctgtgc tgtcggatat
     1741 ctcaatttcc gccgcgttgc gccaggttgg tgtgtcgacc gcccacactt agtcaaactg
     1801 gtcgaaaacc tgtttagccg cgaaagtttt gctcgcaccg aaccgccaaa ggcttgatgc
     1861 gcgatatgtc ctcctgaccc atctcacgtt acaatccgtg gttatgttaa acgcccttct
     1921 ccgtgtgaga gggccttgat cagccaggtt tcctatgaca accgaaacgc gttccctcta
     1981 tagtcaactt ccggctattg atcgcttatt acgcgatagc tccttccttt ctttgcgtga
     2041 tacttatggt cacacccgcg tggtggaatt gttgcgtcag atgctcgacg aagcgcgaga
     2101 agtgattcgt ggcagccaga cgctgcctgc gtggtgtgaa aactgggcgc aagaagtcga
     2161 tgcccggttg acgaaagagg cgcagagcgc gctgcgtccg gtgatcaacc tgacgggaac
     2221 cgtgctgcat accaaccttg ggcgagcttt acaggcggaa gccgcggtgg aagccgttgc
     2281 gcaggctatg cgttcgccag tgaccctcga gtacgatctg gacgacgccg gacgcggaca
     2341 tcgcgatcgg gcgctggcgc agctgctgtg ccgtattacg ggggcggaag atgcctgtat
     2401 cgtcaataac aatgcggcgg cggtgttatt gatgttggcg gccactgcca gcgggaaaga
     2461 ggtggtggta tctcgcggcg aactggtgga gattggcggc gcgtttcgta ttcccgatgt
     2521 tatgcgtcag gcaggctgca ccctacacga agtagggacc accaaccgca cgcacgcgaa
     2581 tgattatcgt caggcggtga atgaaaatac cgcactgttg atgaaagtac ataccagtaa
     2641 ctacagcatt caggggttca ccaaagcgat agacgaagct gaactggtgg cgctcggcaa
     2701 agagctggat gttccagtgg tgactgattt aggcagtggc tcgctggtcg atcttagcca
     2761 gtacggtttg ccgaaagagc caatgccgca ggagttgatt gcggcgggcg tcagtctggt
     2821 gagcttctcc ggcgacaagt tgttaggcgg gccgcaggca gggattatcg ttggtaaaaa
     2881 agagatgatc gcccgcctgc aaagccaccc gctgaagcgt gcattacgcg cggataaaat
     2941 gaccctcgcg gcgctggaag ccacgttgcg tctttattta caccctgaag ctctgagtga
     3001 aaaattaccg accctgcgcc tgcttacccg cagcgcagag gtcattcaaa tccaggcaca
     3061 acgtttacag gctccccttg tcgcacatta cggcgcggag tttgcggtac aggttatgcc
     3121 atgtctttcg cagattggca gtggttcgct gccggttgat cgcctgccga gcgcggcatt
     3181 aacgtttaca ccccatgatg gacgcggtag ccaccttgag tcattagccg cccgctggcg
     3241 tgaattgcca gtgccggtga ttggtcgtat ttatgacgga cgattgtggc tggatttacg
     3301 ctgccttgaa gatgagcaac ggtttttgga gatgttgttg aaatgattat tgcgactgcc
     3361 ggacacgttg accacggcaa aacaacctta ttgcaggcga ttactggcgt aaatgctgac
     3421 cgtctgccgg aagaaaaaaa gcgcggcatg accatcgatc tcggctatgc ctactggccg
     3481 cagccggatg gtcgcgtgcc tggttttatc gacgttcccg gtcatgaaaa gtttctttcc
     3541 aacatgctgg cgggcgttgg tggtatcgat cacgcgctgt tggtggtggc gtgcgatgac
     3601 ggcgtgatgg cacagacccg tgagcatctg gcgattttgc agctgaccgg taacccgatg
     3661 ctgacagtgg cgctgaccaa agccgatcgt gtggacgaag cgcgtgttga tgaggttgaa
     3721 cgccaggtaa aggaggttct gcgggaatac ggttttgctg aggcaaaact gtttatcacc
     3781 gcagcaaccg aaggtcgggg aattgatgcc ctgcgcgagc atctgcttca gttgccggaa
     3841 cgcgagcacg ccagccaaca tagtttccgc ctcgcgattg accgcgcatt taccgtaaaa
     3901 ggtgccgggc tggtcgtcac cggtacggcg ttaagcgggg aagtgaaggt aggcgattca
     3961 ctctggctga ctggtgtaaa taaaccgatg cgtgtacgtg cgctgcatgc gcaaaaccag
     4021 ccaacagaaa ccgccaatgc cgggcagcgt atcgcgcttg acatcgcggg tgatgcggaa
     4081 aaagagcaga ttaaccgtgg cgactggctg cttgccgatg tgccgccaga gccgttcaca
     4141 cgggtgattg tcgagcttca aacccatgca ccgctgaccc agtggcagcc gctgcatatt
     4201 caccacgccg ccagccacgt cacgggacgc gtttcactgc tggaagataa ccttgctgaa
     4261 ctggtcttcg acaccccgtt atggctggca gataacgacc gcctggtatt gcgcgatatc
     4321 tctgcccgca acacgctggc cggagcgcgc gtcgtgatgc ttaacccgcc gcgtcgcggt
     4381 aaacgtaaac cagagtatct gcaatggctg gcttccctcg ccagggcaca gaatgatagc
     4441 gaagcgttag ccgttcacct gcaacgcggc gcggttaatc ttcctgattt cgcctgggcg
     4501 cgccagctca acggcgaagg gatgcgcgaa ctgctgcaac agcctggcta tattcaggct
     4561 ggttatagct tgttgaatgc gccggttgcc gctcgctggc aacggaaaat tctcgacaca
     4621 ttagcgactt atcatgagca acatcgcgat gaacctgggc ctgggcgcga acgtctgcga
     4681 cgtatggcgt tgccaatgga agatgaagcg ctagtactgt tgctgattga aaagatgcgc
     4741 gaaagcggcg acatcctcag tcatcacggt tggctgcatc tgccggatca caaagcgggc
     4801 ttcagcgaag agcagcaggc catctggcaa aaagcagagc cgctgtttgg tgacgagccg
     4861 tggtgggtgc gtgatctggc aaaagagacg ggtaccgacg agcaggcaat gcgcctgacg
     4921 ctacgccagg cggcgcagca aggaataatt accgcgatcg tcaaagatcg ttattaccgt
     4981 aacgatcgga ttgtcgaatt tgccaatatg atccgcgatc tcgatcagga gtgtggttca
     5041 acctgcgcgg cggatttccg cgatcgctta ggcgtaggcc gaaagctggc aattcagatt
     5101 ctggaatatt ttgaccgcat tggctttacg cgtcgtcgtg gaaatgatca tttattgcgc
     5161 gacgcattat tatttccgga aagataagga aatgattaat taatttttaa aataaattaa
     5221 tacaaatttc ttatgatttt aaataaagca cattgtttaa tgaatacaat gtgcttttta
     5281 ttagattaat tttgataatt aataataatg atcatgtttt cgaatgaaaa tcctcagtaa
     5341 gctgcccacc cttttttaca ctttcaggag tgtgttatgg catcttcaac tttctttatt
     5401 ccttctgtga atgtcatcgg cgctgattca ttgactgatg caatgaatat gatggcagat
     5461 tatggattta cccgtacctt aattgtcact gacaatatgt taacgaaatt aggtatggcg
     5521 ggtgatgtgc aaaaagcact ggaagaacgc aatattttta gcgttattta tgatggcacc
     5581 caacctaacc ccaccacgga aaacgtcgcc gcaggtttga aattacttaa agaaaataat
     5641 tgcgatagcg tgatctcctt aggcggtggt tctccacacg actgcgcaaa aggtattgcg
     5701 ctggtggcag ccaatggcgg cgatattcgc gattacgaag gcgttgaccg ctctgcaaaa
     5761 ccgcagctgc cgatgatcgc catcaatacc acggcgggta cggcctctga aatgacccgt
     5821 ttctgcatca tcactgacga agcgcgtcat aacaaaatgg cgattgttga taagcacgtg
     5881 actccgctgc tttctgtcaa tgactcctct ctgatgattg gtatgccgaa gtcactgacc
     5941 gccgcaacgg gtatggatgc cttaacgcac gctatcgaag cgtatgtttc tattgccgcc
     6001 acgccgatca ctgacgcttg tgcactgaaa gccgtgacca tgattgccga aaacctgccg
     6061 ttagccgttg aagatggcag taatgcgaaa gcgcgtgaag caatggctta tgcccagttc
     6121 cttgccggta tggcgttcaa taatgcttct ctgggttatg ttcatgcgat ggcgcaccag
     6181 ctgggcggtt tctacaacct gccacacggt gtatgtaacg ccgttctgct gccgcatgtt
     6241 caggtattca acagcaaagt cgccgctgca cgtctgcgtg actgtgccgc tgcaatgggc
     6301 gtgaacgtga caggtaaaaa cgacgcggaa ggtgctgaag cctgcattaa cgccatccgt
     6361 gaactggcga agaaagtgga tatcccggca ggcctacgcg acctgaacgt gaaagaagaa
     6421 gatttcgcgg ttctggcgac taatgccctg aaagatgcct gtggctttac taatccgatc
     6481 caggcaactc acgaagaaat tgtggtgatt tatcgcgccg caatgtaatc atcattccaa
     6541 caacggctgg caaattgtca gccgcttttt caactatctc tgtaatcctt gcccgtaaat
     6601 tcgtgatagc tgtcgtaaag ctgttaccga ctggcgaaga tttcgccagt cacgtctacc
     6661 cttgttatac ctcacaccgc aaggagacga tcatgaccaa taatccccct tcagcacaga
     6721 ttaagcccgg cgagtatggt ttccccatca agttaaaagc ccgctacgac aactttattg
     6781 gcggtgaatg ggtagcccct gccgatggtg agtattacca gaacctgacg ccggtgaccg
     6841 ggcagctgct gtgcgaagtg gcgtcttcgg gcaaacgaga catcgatctg gcgctggatg
     6901 ctgcgcacaa agtgaaagat aaatgggcgc acacctcggt gcaggatcgc gcggcgattc
     6961 tgtttaagat tgccgatcga atggaacaaa acctcgagct gttagcgaca gctgaaacct
     7021 gggataacgg caaacccatt cgcgaaacca gtgctgcgga tgtaccgctg gcgattgacc
     7081 atttccgcta tttcgcctcg tgtattcggg cgcaggaagg tgggatcagt gaagttgata
     7141 gcgaaaccgt ggcctatcat ttccatgaac cgttaggcgt ggtggggcag attatcccgt
     7201 ggaacttccc gctgctgatg gcgagctgga aaatggctcc cgcgctggcg gcgggcaact
     7261 gtgtggtgct gaaacccgca cgtcttaccc cgctttctgt actgctgcta atggaaattg
     7321 tcggtgattt actgccgccg ggcgtggtga acgtggtcaa tggcgcaggt ggggaaattg
     7381 gcgaatatct ggcgacctcg aaacgcatcg ccaaagtggc gtttaccggc tcaacggaag
     7441 tgggccaaca aattatgcaa tacgcaacgc aaaacattat tccggtgacg ctggagttgg
     7501 gcggtaagtc gccaaatatc ttctttgctg atgtgatgga tgaagaagat gcctttttcg
     7561 ataaagcgct ggaaggcttt gcactgtttg cctttaacca gggcgaagtt tgcacctgtc
     7621 cgagtcgtgc cttagtgcag gagtcaatct acgaacgctt tatggaacgc gccatccgcc
     7681 gtgtcgaaag cattcgcagc ggtaacccgc tcgacagcgt gacgcaaatg ggcgcgcagg
     7741 tttctcacgg gcaactggaa accatcctca actacattga tatcggtaaa aaagagggcg
     7801 ctgacgtgct cacaggcggg cggcgcaagc tgctggaagg tgaactgaaa gacggctact
     7861 acctcgaacc gacgattctg tttggtcaga acaatatgcg ggtgttccag gaggagattt
     7921 ttggcccggt gctggcggtg accaccttca aaacgatgga agaagcgctg gagctggcga
     7981 acgatacgca atatggcctg ggcgcgggcg tctggagccg caacggtaat ctggcctata
     8041 agatggggcg cggcatacag gctgggcgcg tgtggaccaa ctgttatcac gcttacccgg
     8101 cacatgctgc gtttggtggc tacaaacaat caggtatcgg tcgcgaaacc cacaagatga
     8161 tgctggagca ttaccagcaa accaagtgcc tgctggtgag ctactcggat aaaccgttgg
     8221 ggctgttctg atataagaag ctggtcgcat tgggtattca ttgcctgatg cgacgcttac
     8281 gcgtcttatc atgcctacgg gaacctgacc gtaggccgga taaggcgtac acgccgcatc
     8341 cggcataaac aaagcgcacg ttgttacaat ttacgtttta aacctacgtg cgcaataacc
     8401 cagactacgc caacagaagt gcgtttctgg ggcgtgtttt gtcaggagtt taatctcgcg
     8461 tttcgatggt gagcgggcga accaggccgt aaatcaccat tggtgatccg aggccaatca
     8521 gcaataaacg gtagataatt gaaggtttaa tacgcttaat ctgtactgtg gatgttttac
     8581 tggagttatt cattagtttg gccaatagtt ccactatttc aatatgataa acctcgtcct
     8641 gttgaaaaac cagacagcac atttcagaaa gtatataaaa aacaatatgt cgttatataa
     8701 ataaggaaat tgcactgcat catggcattt cataatatgc gttaattgtt cttttttata
     8761 ttctttgagg gaaatatgtt cctggactat tttgcactgg gagtgcttat ttttgtattt
     8821 ctggtgatat tctatgggat tattatttta catgatattc cctacctgat ttctaaaaaa
     8881 cgtaatcatc ctcatgccga cgccattcat gttgctggtt gggtgagtct ttttacgctg
     8941 catgttatct ggccattttt gtggatttgg gccacgcttt accgcccgga gcggggatgg
     9001 ggaatgcaaa tccatgactc ctccgttgtg caactacaac agcgtattgc cgggctggag
     9061 aaacagctcg ccgacatcaa atcctcttct gccgagtaat acttatggat ttactgatta
     9121 ttttgaccta tgtggctttt gcatgggcaa tgtttaagat cttcaaaatt cctgtaaata
     9181 aatggaccat ttccacagcg gccctgggtg gcatatttat tgtcagtggt ttaattctgt
     9241 taatgaacta taaccatccg tataccttca aggcgcaaaa agcggttatt tctattcctg
     9301 ttgtcccaca ggtgacaggc gtggtgatcg aagtgacgga taagaaaaat acgctgatta
     9361 aaaaaggtga ggtgctattt cgactggacc cgacgcgtta tcaggtgcgg gtggatcggc
     9421 tgatggcgga tatcgttacc gcagaacata aacagcgggc gttgggcgca gagttagatg
     9481 agatggcggc gaatactcag caggcaaagg ccacgcggga taaattcgct aaagagtatc
     9541 agcgttacgc acgcggcagc caggcgaaag taaacccgtt ttcagaacgc gatattgatg
     9601 tggcgcggca aaattatctg gcgcaggaag cctccgtaaa gtcatcggcg gcggaacaaa
     9661 aacagatcca gagccagctg gatagcctgg tgttgggtga acattctcaa atcgccagcc
     9721 tgaaagcgca gctcgcggaa gcaaaatata accttgagca gacgatagtg cgtgcgccga
     9781 gcgatggtta tgtgacccag gtgctgattc gtccgggtac ctatgccgcg tcgctgccgc
     9841 tacgtccggt gatggtgttt atacccgatc agaaacgaca aattgtggcg cagttccgtc
     9901 agaactcctt gctgcgactg gcccctggcg acgatgcgga agtggtgttt aatgctctgc
     9961 caggtaaggt attcagcggc aagctggcag ccattagtcc agctgttccc ggcggagctt
    10021 atcagtcgac cggcacctta cagacgttaa acacagcgcc gggttcagat ggcgttatcg
    10081 cgaccattga actggatgag cacactgatt tgagcgcttt accagacggt atttacgccc
    10141 aggtggcggt ctactctgat catttcagcc atgtctcggt gatgcgcaaa gtgctattac
    10201 gcatgaccag ctgggtgcat tacctttatc tcgatcatta atcttcgctt tccgccgctt
    10261 gttcaggccg cagttgctgc gactgagcaa gctggtggcg aatttcacgc agcatttcat
    10321 ccagcacata ctggacccgc cagggcaggt attcccgttt acggaagata gccaccagat
    10381 ccagccacca ttcatacggg tgctgaaagc agggcaccag cgtgccgtct tgcagatcgc
    10441 tctgcacact tttgtctggc gcaaacacaa tccccaggtg atttcgtgcc agctccagcg
    10501 ccgattgcgt gttatcgcaa acgtaatttc ccttcacgcg ataatcccgc acctctttac
    10561 tcccggcgac gttgaagcgc cagatattcg cgtcgtcgat catcatcgag tcgatcaaaa
    10621 tacaggagtg atgttcgagt tcgtcaggac ggctaatagg atgtttctca agatagcgct
    10681 ggctggcata agcggttacg gcatattgtg taataaaact ggcaaccagc gattcatctt
    10741 ttggtggagc gtagctgatt aaaacatcac aatcatcagg aaattcgacg ccttcataaa
    10801 aggcattacg ctcaa
//
LOCUS       DAETPZ010000117        10700 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.118, whole genome shotgun sequence.
ACCESSION   DAETPZ010000117 DAETPZ010000000
VERSION     DAETPZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10700
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.118"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="K0W59_003542"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="K0W59_003542"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBI3024427.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="K0W59_003543"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="K0W59_003543"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HBI3024428.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVTDYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="K0W59_003544"
     CDS             3115..3399
                     /locus_tag="K0W59_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBI3024429.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3449..4102)
                     /gene="fdnI"
                     /locus_tag="K0W59_003545"
     CDS             complement(3449..4102)
                     /gene="fdnI"
                     /locus_tag="K0W59_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HBI3024430.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4095..4979)
                     /gene="fdnH"
                     /locus_tag="K0W59_003546"
     CDS             complement(4095..4979)
                     /gene="fdnH"
                     /locus_tag="K0W59_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HBI3024431.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(4992..8039)
                     /gene="fdnG"
                     /locus_tag="K0W59_003547"
     CDS             complement(4992..8039)
                     /gene="fdnG"
                     /locus_tag="K0W59_003547"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7452..7454),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBI3024432.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8271..9152
                     /gene="yddG"
                     /locus_tag="K0W59_003548"
     CDS             8271..9152
                     /gene="yddG"
                     /locus_tag="K0W59_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HBI3024433.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9411..10511
                     /gene="ompC"
                     /locus_tag="K0W59_003549"
     CDS             9411..10511
                     /gene="ompC"
                     /locus_tag="K0W59_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBI3024434.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT         2623 a         2744 c         2654 g         2679 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc accgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atgggtgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ttataaagcg
     3421 ccattttcag cttaaagttc aaaagagatt atatcccttc ttcactctct tttttcgctt
     3481 ctgccttctc gatttcacga taccagcgcg gatggtgttt cttcgcccag cgacggctca
     3541 ccttcccttc gatcatccct ttaatcgacc ctttcaccca gaatgccata tacatatgga
     3601 tcaggatggc gtggatcagg atgatacccg cagccgcgtg gatcagcagg ctgtagcgaa
     3661 caacctgcat cgggaagtac tgcgcaaagt acggacgcca gataatcacc ccggtcacca
     3721 gcagcacgaa aatcatgctc atgatcgacc agaacatcat cttttgcccg gcgttgtact
     3781 taccaacatc cgccactttg tgctcattgc cttttaatac ttcgacaatg ttcaacagcc
     3841 acggaatatc tttcttatcc gggatgttgt gatgcacaaa acgcacaaac ataaacatca
     3901 gtgcgacgaa aatcgcaatg ccgaagaacg ggtgcaaaat gcgtcccatc tgcggcgtac
     3961 cgaaggtttg cgtcagccat tgcagcgtcg ggaagaagaa cgaaatcccg gacagcgcca
     4021 ccaggaagaa gcaaatcacc acggtccagt gacaggcgcg atcaataaat ttggtgcgca
     4081 caatcatttt cgacttactc atgatgatcc tcctcgtcat cgtccacttc cttattcggg
     4141 ccaataccga tgtagtggaa aatcaacccg gcaaaagtgg caataaagcc agccgctgcc
     4201 agcggtttca acgcgccttt ccacaggctt accgaggtgt cgatcttcgg atctttcggc
     4261 agaccgtgat acagctccgg ctgatcggcg tgatgcagca cgtacataac gtgcgtacca
     4321 ccgacccctt ccgggttgta gacgccagca tgttcgtaac cacgcgcttt cagtttcgcc
     4381 acgcgctgtt ccgccagctc cagcatctcc ttcttggtgc cgaagtggat agccccggtc
     4441 ggacaggttt tcacacaagc cggttcctgg ccgacgctga cgcgatcgac gcagagcgtg
     4501 catttatata cccggttatc ctctttgttg aggcgcggaa tattaaacgg acacccggca
     4561 atgcagtaac cacagccgat gcagttttcc gactggaaat cgacaatccc gttagcgtac
     4621 tgaatgattg caccagcaga cgggcacgcc ttcaggcagc ccggatcttc acagtgcata
     4681 cagccgtctt tacggatcag ccactccagc ttgccattct gttcggtttc gctaaagcgc
     4741 atcaccgtcc aggacttggc gctcagatcg gcggggttat cgtaaacacc gacgcagtgc
     4801 cccacttcat cacggatgtc gttccactcc gaacacgcca cctgacaggc tttacagccg
     4861 atacaggtgg aaacgtcgat aagtttcgcg acttctgctt tgtaatcacg cacctgagaa
     4921 ggcggcgtga tggagttagt tgcggacctt ttgataatat cctgcgtttc catagccatc
     4981 tgttcgcccc cttacgcctt ctcgatgttg actaagaacg ctttatattc cggcgtttgc
     5041 gagtttgcat caccgacatt cggcgtcaga gtgttagcga tataaccttt acgcgcgaca
     5101 ccctcaaagc cccagtggat tggaataccc accgtttcaa cctgctgacc gtttacattc
     5161 agcggtttca gacggcgcgt taccactgcc accgcgcgga taaagcctcg cttgctggag
     5221 acagtgacac gatcgccatt attaatgcct ttcgccgccg cctgcgtttc gctgatttcc
     5281 acaaactgtt ccggctgagc aattgcgttg agcaacgcgt gcttggtcca ggtgtggaag
     5341 tgctcggtca gacgataggt cgtacccaca tacgggaact gctctttttt acccatccgc
     5401 agcgcgtctt gttcatacag acgaacaacc gggttagaca ccacgttcgg atgcagcggg
     5461 ttagtgccca gcggcgtttc aatcggctcg tagtgttccg ggaacggacc ttccgccatt
     5521 ttgttgatgg caaacaggcg tcccatccct tccggctgca taataaacgg cccggttggc
     5581 gtacccggtg cggcattgcc gaagtccgga atatcgttac ccgtccactt gctgccgttc
     5641 cactggatca gcatccgttt cggatcccac ggtttaccgt tgatatccgc cgaagcacgg
     5701 ttgtagagta cgcgacggtt gagcggccac gcccaggccc atcccagcgt attccccaga
     5761 ccggacgggt cggagttatc gcggtttgcc atctggttac cctgctctgt ccagctaccg
     5821 gtgtagatcc agcaagaaga tgcggttgta ccgtcatcac gcagatgcgc aaagctactc
     5881 agcaactgac ctttcttcgc aatcagcacg ccattagcat catagagatc ttccaacgca
     5941 tagccgttgt tctctttagc tacttcgtca gattgcggtt cgtgcggctg cttgtagttc
     6001 cagctcatct tcatcagcgg ttctacgcct ttaccacctt cggcctggta caactcgcgc
     6061 agatgatggt agatacccgc cagaatttcg ccgtcgttac gcgcttcgcc cggcgcgtcc
     6121 tgacctttcc agtgccactg caaccagcga ccggagttag cgatagaacc atcctcttca
     6181 gcaaagcagg tcgaaggcag acggaatact tcagtctgaa tagacgccgg atcgacatcg
     6241 ttcgactcgc cgtggttctg ccagaaggta gaggtttcag tcaccagcgg atcgataacc
     6301 accatgtact tcagtttgct caggcagctc accactttgt ttttatccgg gaaggacgca
     6361 accgggttaa agccctggca gaaataaccg gtgactttgc cttcatccat catgttgaaa
     6421 tacttgatga cgtcgtaggt ctggtcccac ttcggcagcc agtcatagcc ccagttgttc
     6481 tctttctgcg cggcatcgcc atagaaagat ttcatcaggc taacgaagaa cttcggatag
     6541 ttgctccagt agttcacctg atcagccagc gtcgctttcg gcgtgttcgc ttccagatac
     6601 gactgcaaat caacctgttt ttctgacggc agcgtcagat aacctggcag gctggtagag
     6661 agcagaccta agtcagtcag cccctgaatg ttggagtgac cacgcaatgc gttcacgccg
     6721 ccaccggcca tacccatgtt accgagcagc aactggatca tcgccatagt acggatgttc
     6781 tgcgcaccca cggtgtgctg cgtccagccc agcgcgtaca ggaaggtggt tgtgcgatcc
     6841 ggtgcgctgg tggaggccag cacttcacac actttcagga agtcggcttt tggcgtacca
     6901 cagatgtttt caacaacgtc cggcgtgtag cgggaaacgt gcgctttcag caggttccac
     6961 acacagcgcg gatgagtcag tgtttcatcg cgtttcgcat agccgttttc atcgaactga
     7021 tagttccagg acgatttgtc gtactggcgt ttttcagcgt cataaccgct gaacaggcca
     7081 tcttcgaaag caaaatcatc acgcaccagt aggctggcgt tggtgtaatg tttaacgtat
     7141 tcggcgttga ttttgttgtt ttcgatcagg tagcgcaaaa cgccagacag gaacgtaatg
     7201 tccgtaccgg aacgaatagg tgcgtaaata tccgccacag aagcggtacg cgtaaaacgg
     7261 ggatcgacaa cgatcaaggt tgcatcgttg ttgtttttcg cttccatcgc ccagcggaaa
     7321 ccgacgggat gcgcttcagc agcgttaccg cccattacca ttacgacgtt agcgtttttg
     7381 atatccaccc agtggttggt catagcaccg cgaccaaatg ttggagcaag acttgctacc
     7441 gttggtccgt gtcagacgcg cgcctggttg tctaccgcca gcatcccgag ggagcgggca
     7501 aatttctggg tcagcatccc ggtttcgttg ctggcaccgg aggcacacag cataccggta
     7561 gaaagccaac ggtttaccgt tacgccctgc tcgttctttt caataaagtt agcgtcacgg
     7621 tcagctttca tcagcttcgc aatacgggag aatgcttctt cccagctaat gcgctgccat
     7681 ttgtcagaac ctggcgcacg atattccggg tagcgcagac ggttttcact gttgacgtaa
     7741 tccagcaaac cggccccttt cgggcacagc gcaccacggc ttaccggatg atccgggtca
     7801 ccttcaatgt gataaatcgc ttctctggcg ttttttgcgc catcacccag gctatacatc
     7861 aatagcccgc aacctacgga acagtatgtg caggtgttac ggatctcttt agcgcgtaat
     7921 aatttgtagt ttcgcgcctg agccagtgct tgcttcgggg caaagcccaa tgccgctacc
     7981 gttgttccag ccatacctcc cgcgcagatt ttaaaaaatt gtctgcgact gacgtccatt
     8041 gctttcctct tttttcaggg gtattactgc gcgaggaaac taacagtaaa attctgaatc
     8101 tttttgcgcg aaatcaaaaa gaacacgtgt ttgccacaaa ataatccgcc tgcgcaaatt
     8161 gacctacctc aatagcggta gaaaaacgca ccactgcctg acaggccagt taaaaaaatg
     8221 ctataaaatt cagcttaatt tttaacggca agagagacaa aacagcgagc atgacacgac
     8281 aaaaagcaac gctcataggg ctaatagcga tcgtcctgtg gagcacgatg gtaggattga
     8341 ttcgcggtgt cagcgagggg ctcggcccgg tcggcggcgc agctgctatc tattcattaa
     8401 gcgggctgct gttaatcttc acggttggat ttccgcgtat tcggcagatt ccgaaaggct
     8461 atttactcgc cgggagtctg ttattcgtca gctatgaaat ctgtctggcg ctttccttag
     8521 ggtatgcggc gacccgtcat caggcgattg aagtgggtat ggtgaactat ctgtggccca
     8581 gcctgacaat tctctttgcc attctgttta atggtcagaa aaccaactgg ctgattgtac
     8641 caggattatt attagccctg gtcggcgtct gctgggtgtt aggcggtgag aatgggttac
     8701 attatgatga aatcatcaat aatatcacca ccagcccatt gagttatttc ctggcgttta
     8761 ttggtgcgtt tatctgggca gcctattgca cagtaacgaa taaatacgca cgcggattta
     8821 acggaattac cgtttttgtc ctgctaacgg gagcatgtct gtggatttac tattttctca
     8881 cgccacaacc agaaatggta tttagcacgc ccgtcatgat taaactcatc tctgcggcat
     8941 ttaccttagg atttgcttat gctgcatgga atgtcggtat attgcatggc aatgtcacca
     9001 taatggcggt aggctcgtat tttacgcctg tactttcctc agcgcttgca gccgtgctgc
     9061 tcagcgcccc gctgtcgttc tcgttctggc aaggcgcgct aatggtctgc ggcggttccc
     9121 tgctctgctg gctggcgaca cgtcgtggtt aaaataattg ccgggtctac ggcccggcag
     9181 ttaatcagat aaattccgac aatattaaaa aataaatttc aaataccagt aattcacctt
     9241 atatctcatt tcgacttcga tcacatatcg ataaataata ttatttcatc tatttttctc
     9301 attgttttta atcaccattc tattttaaaa acagtatgtc atttataaat gacgccgttt
     9361 aaaattcgtt tagaaaatac gtttaacgat aattaaaagg attctgttaa atgaaattaa
     9421 aaatagttgc ggtggttgta actggtttgt tagctgcgaa cgtagcacac gctgccgaag
     9481 tctataacaa ggatggtaat aaactcgacc tttatggcaa ggttaccgct ctacgttatt
     9541 ttactgatga taagcgtgac gatggtgata aaacttatgc ccgtctcggc tttaaaggag
     9601 aaacgcaaat caatgatcaa atgattggtt ttggtcactg ggaatatgat tttaaaggct
     9661 ataacgatga agccaacggc tcgcgcggca acaagacccg tctggcctat gctggtttaa
     9721 aaattagtga atttggctct ctggactatg gtcgtaacta cggtgtcggc tacgacattg
     9781 gttcatggac cgatatgttg ccagaatttg gtggcgatac ctggagtcag aaagatgtct
     9841 tcatgacata ccgtaccacc ggtctagcaa cctatcgcaa ctacgatttc tttggcttaa
     9901 ttgaagggct gaactttgcc gcgcaatatc aaggcaaaaa tgaacgtact gacaacagtc
     9961 atctttatgg tgctgactac acgcgtgcca acggtgacgg tttcggtatc tcctcaactt
    10021 atgtttatga tggctttggt atcggtgcgg tatataccaa atccgatcgg acaaatgcgc
    10081 aggaaagagc cgctgctaat cctctcaatg cctccggtaa gaatgcagaa ctatgggcca
    10141 caggtataaa gtatgatgcc aacaacatct actttgcagc taattacgct gaaacattaa
    10201 acatgaccac ctatggggat ggttatatct ctaacaaagc acaaagtttt gaagtggtgg
    10261 cgcaatatca attcgacttc ggcttgcgcc catcactcgc ttacctgaaa tcgaaaggca
    10321 gagatctggg ccgctacgcc gaccaggaca tgattgagta tatcgacgtt ggtgcgacgt
    10381 atttcttcaa caaaaatatg tcgacctatg ttgattataa aatcaacctg attgatgaaa
    10441 gcgactttac ccgtgccgta gatattcgca ccgataacat cgtcgctacg ggcattacct
    10501 atcagttcta acgtcctctc ctgaccagcc cttctctggg ctggtttttg atttaagtgc
    10561 tgctgtatga ctccttaagt aaaaatgagc aatttttgat atttctggtc attcacctca
    10621 attaatatta ttccatatcc tccggggaca tcttactata atatttattg atgagtgatt
    10681 atgaccaata ttaatacagc
//
LOCUS       DAETPZ010000118        10651 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.119, whole genome shotgun sequence.
ACCESSION   DAETPZ010000118 DAETPZ010000000
VERSION     DAETPZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10651
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.119"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(57..1706)
                     /locus_tag="K0W59_003550"
     CDS             complement(57..1706)
                     /locus_tag="K0W59_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBI3024435.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            2111..3508
                     /gene="ccp"
                     /locus_tag="K0W59_003551"
     CDS             2111..3508
                     /gene="ccp"
                     /locus_tag="K0W59_003551"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HBI3024436.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLVLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            3719..5119
                     /locus_tag="K0W59_003552"
     CDS             3719..5119
                     /locus_tag="K0W59_003552"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBI3024437.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            5489..6313
                     /gene="gadX"
                     /locus_tag="K0W59_003553"
                     /pseudo
     CDS             5489..6313
                     /gene="gadX"
                     /locus_tag="K0W59_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
     gene            6681..7409
                     /gene="gadW"
                     /locus_tag="K0W59_003554"
     CDS             6681..7409
                     /gene="gadW"
                     /locus_tag="K0W59_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HBI3024438.1"
                     /translation="MTYVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(7772..>10651)
                     /gene="mdtF"
                     /locus_tag="K0W59_003555"
     CDS             complement(7772..>10651)
                     /gene="mdtF"
                     /locus_tag="K0W59_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HBI3024439.1"
                     /translation="SSTSDAAGNASITLTFETGTSPDIAQVQVQNKLQLAMPSLPEAV
                     QQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVASNIKDPLSRTAGVGSVQLFGS
                     EYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQLGGMPQVADQQLNASIIVQT
                     RLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYSTVARYNGKPAAGIAIKLAAG
                     ANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIEISIQEVFKTLVEAIILVFLV
                     MYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINTLTMFGMVLAIGLLVDDAIVV
                     VENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLSAVFMPMAFMSGATGEIYRQF
                     SITLISSMRLSVFVAMSLTPALCATILKAAPEGGHKPNALFARFNTLFEKSTQHYTDS
                     TRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEEDQGVFMTTAQLPSGATMVNTT
                     KVLQQVTDYYLTKEKDNVQSVFTVGSFGFSGQGQNNGLAFISLKPWSERVGEENSVTA
                     IIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMELLDNGNLGHEKLTQARNELLS
                     LAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVALSDINQTISTAFGISYVNDF
                     LNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAPLSAYSSTEWTYGSPRLERYN
                     GIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYSWTGLSYQEALSSNQAPALYA
                     ISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLATDLRGLSNDVYFQVGLLTTIG
                     LSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRPILMTSLAFILGVLPLVISHG
                     AGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHLFARFKKA"
BASE COUNT         2727 a         2526 c         2626 g         2772 t
ORIGIN      
        1 tcctgaagag gataatttta cagccagcag gaagttagcg gctggctgta aaaatattat
       61 ggttcgccgt acaaaccaat taaacggcgt accacaccgt tagtccagcc aaacccatcc
      121 tgcaacggat actcgccacc gccgccttcg cggggaacac catcggcaat atggtatttc
      181 tcgatcattt tgtgctgttc cagatagaac tgattcaccg ttttcagcca gctgcgcgcg
      241 atttcatcac ccagaaggtc atcgccatac attttaaatc cctgaattgc catccattgt
      301 aacggtgccc agccgttggg tttatcccac tgttcaccgg tttcgtactc gcttgccaaa
      361 atcccgccag gtgtcagtaa ccggctgcgt acggcgtttg ccagacgatc ggcctgttca
      421 tggttcgcca tgccgacata gagcggcaca atggcggcag cggaaaacag cgccagttgt
      481 tcgcgtcgcc agtcgtaatc gcggtagatg ccgttttcat catcccagag gtaacggttt
      541 accgcatcgc gacgggcact ggccttctgg cggaacagcg cttctgtctc tttctcgcct
      601 ttcagcgccg agatgttggc gatggcgctc tccagtttaa acaggaaggc attcagatcg
      661 atggggatga actgggtggt acgaatgctc gccagacgac cagtatcacg cagccaacgg
      721 gaagagtaat cccaaccgga ggccgctccc gcgcgtaaat cgcggtacac ctcgttgggc
      781 gggcgaccag aatgtttcgc ggtttcaacg tcctcaagcc aggattcgtc acgcggcgtg
      841 tcgcgatcgt cccagtaacg gttgagcagc gatccgtccg gcatccgcac aacatggcga
      901 taggcctgat tagggatcaa cgattctgca ccgtccatcc agaaggcata ttccatttta
      961 aggtggtcga gatagcggcg cgcaccgcgt acaccatctt cttcaaacaa ctccaccatc
     1021 agcgcaaaaa ccggtggttg cgagcggctc aaataatagg tgcggttgcc gttggggatg
     1081 tgaccgtagt tttcgatcat ccaggcgaag ttatcggcca tgcatttcag caaatcttcc
     1141 cgaccacttt ccgccagtcc cagcatggtg aaataggaat cccaatagta ggtttcgcta
     1201 aaacggccgc ccgggacaat atatgactgc ggtagcgcca gcagagaaga ccacggaatg
     1261 tgatcctgtg gttcgcgggt tagcaccggc cacagctggt cgatatgctc tttcagggaa
     1321 ttttgcgggt ccgatacata ctcgctggag tagacctccg gcagccagaa gtgattttca
     1381 acaaacttgc gcaagtcaaa atcacgatga cggcgcactt tacggtagcg gattaaaata
     1441 tccagcgggt ccattttggg tgcgcagtcg ggaaaggttt tactgtcggg gaaaattttc
     1501 gccgactgaa catgctcgaa cagttcgaga tagcgatcgg caggcgtcag cgcatcagag
     1561 gcgggcagcc cttcaatcat ctcgggttcc ggttctgcct cgatcatctc atccagtttt
     1621 aattcatacg ggtccagttc atagcagaga tcgacctcga tcatcagttc gtctggatta
     1681 gggttttgaa ttttctgatt gagcataagg atccaacctc cgaaattcgt cgctaattat
     1741 cgggcttttc tttaagggta gtcattcgct ccgcctggtg ggcgacaaag cgtgcggtgg
     1801 atcacgttta ttaacttgtc aaaaaatata acgcactaat atcaatggaa tttataaaaa
     1861 tacggatttg gtgttgtttt tcattttcag aacattcaat cctgctctcg ccgcaatcat
     1921 gatatgccat tcctgatctg ggtaataggc acaggctatc ttattgatag tttatattca
     1981 tgtaattgat tgattcctat caaaaagcgt atctccctgt tttttatgat ggctttgtct
     2041 cgcagagacg tttgcagcat tctggaaaat tttctgggtc acaataactc accaccgtga
     2101 gggaagaata atgaaaatgg tctcacgtat taccgcgatc ggcctggctg gcgtcgcgat
     2161 ttgctattta gggttatctg gttatgtgtg gtaccacgat aataaacgca gtaaacaggc
     2221 cgatgttcag gcatctgctg tcagtgaaaa taataaggtt ttaggctttc tccgcgaaaa
     2281 aggatgcgac tattgccaca cgccttcggc agaattaccc gcctattatt atattcctgg
     2341 cgcgaaacag ttgatggatt acgacattaa gcttggatat aaatctttta acctcgaggc
     2401 cgtgcgtgcg gcactgctgg ctgataaacc cgtttcgcaa agcgatctga ataagattga
     2461 atgggtgatg cagtatgaaa ctatgccacc aacgcgttat accgcgctac actgggcggg
     2521 taaggtgagt gatgaagagc gggcggaaat actggcctgg attgcaaaac agcgcgcgga
     2581 atattacgcc agcaatgata ctgctccgga gcatcgcaat gaaccggtgc agcccatccc
     2641 gcaaaaactg cctaccgatg cgcaaaaagt ggcgttgggc tttgcgctgt atcacgatcc
     2701 ccgtttatcg gctgatagca ccatttcatg cgctcattgc catacgttga atgcgggggg
     2761 cgtcgatggc agaaaaacat cgattggtgt tggtggcgca gttgggccga ttaatgcgcc
     2821 gacggtattt aactcagtat ttaacgttga gcagttctgg gatggtcgtg cggcaacatt
     2881 gcaggatcag gcaggtggac cgccgttgaa cccgattgaa atggcgtcga aatcctggga
     2941 cgaaattatt gctaagctgg aaaaagatcc gcagcttaaa gcgcagttcc tcgaagttta
     3001 tccgcaaggt ttcagtggcg aaaatattac tgatgccatt gctgaatttg agaaaacatt
     3061 aattacgccg gattccccat ttgataaatg gttgcgtgga gatgaaaatg cgctgacggc
     3121 gcaacagaaa aaaggctatc aattatttaa agataataaa tgtgcaactt gtcatggtgg
     3181 tattattctc ggcggacgtt cctttgaacc gttggtgctg aaaaaagact ttaactttgg
     3241 ggaaattacg gcggcggata ttggtcgtat gaatgtgact aaagaagagc gtgataaatt
     3301 gcgtcagaaa gtacccggtt tacgtaacgt tgctttaacg gcaccgtact tccatcgcgg
     3361 tgacgtgccg acgctggacg gggcggtgaa actgatgctg cgctatcagg taggcaaaga
     3421 gctgccgcag gaggatgtgg atgatatcgt agctttcctg cacagtctga acggggtgta
     3481 cacgccgtat atgcaggata aacaataatt aatttgatcg cccgaacagg aatgtttggg
     3541 cgatttttat tacgataata aagtctgttt ttaatattat catgttaaat gtttatatta
     3601 taaaaagtcg tttttctgct taggattttg ttatttaaat taagcctgta atgccttgct
     3661 tccattgcgg ataaatccta cttttttatt gccttcaaat aaatttaagg agttcgaaat
     3721 ggaccagaag ctgttaacgg atttccgctc agaactactc gattcacgtt ttggcgcaaa
     3781 ggccatttct actatcgcgg agtcaaaacg atttccgctg cacgaaatgc gcgatgatgt
     3841 cgcattccag attatcaatg atgaattata tcttgatggc aacgctcgtc agaacctagc
     3901 cactttctgc cagacctggg acgacgaaaa cgtccataaa ttgatggatt tgtcgatcaa
     3961 taaaaactgg atcgacaaag aagaatatcc gcaatccgca gccatcgacc tgcgttgcgt
     4021 aaatatggtt gccgatctgt ggcatgcgcc tgcgccgaaa aatggtcagg ccgttggcac
     4081 caacaccatt ggttcttccg aggcctgtat gctcggcggg atggcgatga aatggcgttg
     4141 gcgcaagcgt atggaagctg caggcaaacc aacggataaa ccaaacctgg tgtgcggtcc
     4201 ggtacaaatc tgctggcata aattcgcccg ctactgggat gtggagctgc gtgagatccc
     4261 tatgcgcccc ggtcagttgt ttatggaccc gaaacgcatg attgaagcct gtgacgaaaa
     4321 caccatcggc gtggtgccga ctttcggcgt gacctacacc ggtaactatg agttcccaca
     4381 accgctgcac gatgcgctgg ataaattcca ggccgacacc ggtatcgaca tcgacatgca
     4441 catcgacgct gccagcggtg gcttcctggc accgttcgtc gccccggata tcgtctggga
     4501 cttccgcctg ccgcgtgtga aatcgatcag tgcttcaggc cataaattcg gtctggctcc
     4561 gctgggctgc ggctgggtta tctggcgtga cgaagaagcg ctgccgcagg aactggtgtt
     4621 caacgttgac tacctgggtg gtcaaattgg tacttttgcc atcaacttct cccgcccggc
     4681 gggtcaggta attgcacagt actatgaatt cctgcgcctc ggtcgtgaag gctataccaa
     4741 agtacagaac gcctcttacc aggttgccgc ttatctggcg gatgaaatcg ccaaacaggg
     4801 gccgtatgag ttcatctgta cgggtcgccc ggacgaaggc atcccggcgg tttgcttcaa
     4861 actgaaagat ggtgaagatc cgggatacac cctgtacgac ctctctgaac gtctgcgtct
     4921 gcgcggctgg caggttccgg ccttcactct cggcggtgaa gccaccgaca tcgtggtgat
     4981 gcgcattatg tgtcgtcgcg gcttcgaaat ggactttgct gaactgttgc tggaagacta
     5041 caaagcctcc ctgaaatatc tcagcgatca cccgaaactg cagggtattg cccagcagaa
     5101 cagctttaaa cacacctgat aacataacgt tgtaaaaacc gaatgcccag cctttaaaaa
     5161 aacagctggg cattcggttg cttattaatc gcaataatat attggctgtc tattcatcgt
     5221 gttgataaga tatatgttat atgaatgttt attaaatgta gcgattacct ttaatgtcac
     5281 gaaaagggat tatttgcttg ctattaattt cactgcgttc ttaaacacgt ttttgtatta
     5341 taaatccagt catcctgccc gtagaacatc acacattatc atcctgttct cccgcttcgt
     5401 ttaaatttat ttatcaatca atttgactta agagggcggc gtgctacatt aattaacagt
     5461 aatatgttta tgtaatatta agtcaactat gcaatcatta catgggaatt gtctaattgc
     5521 gtacgcaaga cataaatata ttctcaccat ggttaatggt gaatatcgct attttaatgg
     5581 cggtgacctg gtttttgcgg atgcaagcca aattcgagta gataagtgtg ttgaaaattt
     5641 tgtattagta tcaagggata cgctttcatt atttctgccg atgctcaagg aggaggcatt
     5701 aaatcttcat gcacataaaa aaatttcttc attactcgtt catcactgta gcagagatat
     5761 tcctgttttt caggaagttg cgcaactatc gcagaataag aatcttcgct atgcagaaat
     5821 gctacgtaaa agagcattaa tctttgcgtt gttatctgtt tttcttgagg atgagcactt
     5881 tataccgctg cttttgaacg ttttgcaacc gaacatgcga acacgagttt gtacggttat
     5941 caataataat atcgcccatg agtggacact agcccgaatc gccagcgagc tgttgatgag
     6001 tccaagcctg ttaaagaaaa aattgcgcga agaagagaca tcatattcac agttgcttac
     6061 tgagtgtaga atgcaacgtg ctttgcaact tattgttata catggctttt caattaagcg
     6121 agtcgcagtg tcctgtggat atcacagcgt gtcgtatttc atttacgtct ttcgaaatta
     6181 ttatgggatg acgcccacag agtatcagga gcgatcggcg tagggattgc cgaaccgtga
     6241 ctcggcggca agtattgttg cgcaagggaa tttttacggc actaaccgtt ctgcggaagg
     6301 aataagatta tagagtttta ctcagacata aaaaaaaccc ggcatagggg accgggaaga
     6361 ggatagtctg ccgtctccag actaataaac cgttataaca ctccctgttg gcacgggaaa
     6421 ctttgtgctc tcagtaagtt aaatataact tttactggaa ataagatcag ccattttttt
     6481 ataaacataa gctatacgct gtgcgaaaat atattcgtgc tgcatttact tattatcaat
     6541 taactgttat gcaaaactac tttgtggata aattttggtc ctaccaaatc tggcagtttt
     6601 tgcgctaaga aacagtctgt catcatttca ttagtatact gaaattgaaa taatcgcagt
     6661 atgaaatata agggataatc atgacttatg tctgctcggt gatcctcatt cgtcgttcat
     6721 tcgatattta tcatgaacag caaaaaatat cgctgcataa cgagagtatc ctgctgctgg
     6781 agaaaaattt ggcagacgat tttgcgtttt gttcaccgga tacgcgacga ctggatatcg
     6841 atgagctgac agtttgccat tacttacaaa atattcgtca gctaccacgc aatttagggt
     6901 tacatagcaa agaccgtctg ttaattaacc agtcaccccc catgccgctg gtgacggcga
     6961 tttttgatag cttcaatgaa tccggggtaa attcaccgat actgagcaat atgctctacc
     7021 tttcctgttt atcgatgttt tctcataaga aagaactgat ccccttactt ttcaatagca
     7081 tcagcactgt ttcaggaaaa gttgaacgcc ttattagctt tgatatcgcc aaacgttggt
     7141 atctgcgcga tatcgcggaa agaatgtata ccagcgagag tctcatcaaa aaaaagttgc
     7201 aggatgaaaa tacctgtttc agtaaaatat tactcgcttc caggatgtcg atggccagac
     7261 gattactcga gttacgtcaa attcctctgc atactattgc ggaaaaatgt ggctatagca
     7321 gtacgtcgta ctttataaac acatttcgac aatattatgg tgtaacgcca catcagtttg
     7381 cgcaacattc gccaggtacc ttttcctgac atattttgca tttgaatatt ggtcaggatc
     7441 tcacacctgc ttcatgtgaa actcttccct gatgatttct gccgggctac cggctagttc
     7501 tctttcgcag tttgccaggc tgtataggta ttttcaggag gaaaagactc ggcatgtttg
     7561 ggattattaa gctgacaatt catacgatta cgggaatgtg ggtcagtatt gtgttgttca
     7621 aattgatgac gaatggctgg agtggtttct actttcagtg ctgtgtacta agcctggtat
     7681 tcctgacggt tagctggttg ttgagtggtg aatggttagc aggaaagagt aaggctgaac
     7741 cttcacgttc aaccttactc tcatttacac gttacgcttt tttaaagcgg gcaaagagat
     7801 gttccactac aacgaaaaag accggaacga agtaaattgc cagcactgtt gcggcaaaca
     7861 tcccgcccat cacgccggta cctaccgcgt tttgcgcgcc agaaccggca ccatgactga
     7921 taaccagcgg cagcacgccg agaataaagg ccagagaggt catcaggatt gggcgtaaac
     7981 gcatccgcgc cgcttcgatg attgcctcta tcggcgtttt cccttctttc tgcatcatct
     8041 caacggcaaa ttcgacaatc aggatggcgt tcttggcgga aagtccgatg gtggtcagca
     8101 aaccaacctg gaagtagacg tcattactta agccgcgcag atcggtggcc agtaatgcgc
     8161 caacgacgcc taacggaaca accaacatca ccgagaacgg aattgaccaa ctctcataga
     8221 gtgcggcgag ggcgaggaac accacgacca gtgaaatggc atacagtgca ggagcctggt
     8281 ttgaggataa cgcttcctga tacgacagtc cggtccatga gtagccgacg cctgccggaa
     8341 gtttagcgac caggtctgcc ataaatttca tagcgtcacc ggtacttttc ccggccgccg
     8401 cttcacctaa aatctccatt gacgggatgc cgttgtagcg ttccaatcgc ggtgaaccat
     8461 aggtccattc ggtagacgag taggcagaaa gcggtgccat cgtgccagag gcgttgcgta
     8521 cataccattg gttgatgtta tccggcaaca tacggaacgg cgtgcctgcc tggacataca
     8581 cttttttcac ccgcccctgg ttgaggaagt cgttcacgta gctgatgccg aaggcggtgg
     8641 aaattgtctg gttgatatca gacagcgcca cacccattgc ctcagctttc gcggcgttga
     8701 cgttcacttt gaacatcggc gtatcttcca ggccgttcgg gcgtaccccg gtgacctggt
     8761 caggcgattg cgctgccagt gataacagct cgtttcgtgc ctgggttagt ttttcgtgcc
     8821 ccaggttacc gttgtccagc agttccatat caaaacctga cgcggtaccc agttcagcca
     8881 ccgcgggtaa gttgaacggg aagacgacgg ctttattgat actgcttaac gcaatcattg
     8941 cccgctgaat gatcgcggta accgagtttt cctcaccgac acgttcagac cacggcttga
     9001 gactgataaa cgccaggccg ttgttttgcc cctggccgct gaagccaaag ctgccaacgg
     9061 taaacaccga ctgaacatta tctttctctt ttgtaagata ataatccgtc acctgttgca
     9121 gtactttcgt ggtgttaacc atggtggcac cggaaggtaa ctgcgcggtg gtcataaata
     9181 ccccctgatc ctcttctggt aagaaagagg tcggtgtacg caggaacagc accgccatcc
     9241 cggcgcaaat cagcaggtag accaccatgt agcgaccggt acaacgcaac agcgagcggg
     9301 tgctatcggt atagtgctga gttgattttt caaacagcgt gttgaagcgt gcgaacaggg
     9361 cgttaggttt gtgaccgcct tccggcgcgg ctttcagaat ggtggcgcac agggcagggg
     9421 tcaggctcat tgccacaaat actgaaagcc gcatggagga gatcagcgtg atggagaact
     9481 ggcggtagat ctccccggtt gcaccgctca taaaggccat cggcataaac actgcagaaa
     9541 gaacaacggc aatcccgacc agcgcacgtt ggatctgccc catcgattta tgcgtcgctt
     9601 ccttcggcgg tagcttatct tccgcaatga cacgctcgac gttctccacc accacgatgg
     9661 cgtcatccac cagtaaccct atcgccagca ccatcccgaa catagtcaac gtgttgatgg
     9721 tgaaaccgac cgccgacaag atcgcaaacg tcccgagaat aaccaccggt acggcaattg
     9781 tcgggatgat ggtggcacgg aaattctgca aaaacagata catgaccagg aagacgagga
     9841 tgatagcctc aaccagtgtt ttgaaaactt cctgaataga aatttcgata aacggcgtgg
     9901 tgtcgtaagg ataaaccgtc ttcagacttg cggggaaata ggctgataag cggttcagct
     9961 cctctttgac tgcccgcgag gtatccaggg cgtttgctcc ggcagccagt ttgatggcga
    10021 tcccggcagc aggtttgccg ttatagcgcg ccacggtgga ataatcttcc gccccaagtt
    10081 cgacgcgggc gacatcacgc agcagcactt gcgaaccatc ttgctgaact ttcaacagga
    10141 ttttgccaaa ttcttccggc gtttgcagac gcgtctgcac aatgatcgag gcgtttagtt
    10201 gctggtctgc cacctgtggc atgccaccca gttgaccacc agaaatctgg ttgttttgca
    10261 ccttaatctg ggaaataaca tcggaaggta ccaggttata tttattgagt ttttgcgggt
    10321 ccagccagat acgcatagcg tactcggaac caaagagctg tacgctacca acgcccgcgg
    10381 tacggcttag cgggtctttt atattagacg ctacatagtc cgcgatatcg tactggttga
    10441 ggctgccgtt atcagaaata aacgccgcta ccatcaggat attactgctc gacttatcga
    10501 cgctaatccc ctgttgctgc accgcttcag gtaatgaagg catagcgagt tgcagtttat
    10561 tttgcacctg aacctgtgcg atatcaggag atgtcccagt ctcgaaggtc agagtgatag
    10621 aggcattacc cgccgcatca ctggttgaag a
//
LOCUS       DAETPZ010000119        10215 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.120, whole genome shotgun sequence.
ACCESSION   DAETPZ010000119 DAETPZ010000000
VERSION     DAETPZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10215
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.120"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(296..1156)
                     /locus_tag="K0W59_003556"
     CDS             complement(296..1156)
                     /locus_tag="K0W59_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBI3024440.1"
                     /translation="MALDAGTGSIRAVIFDLEGNQIAVGQAEWRHLAVPDVPGSMEFD
                     LNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNEGTPIWACANVDARA
                     AREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSDIYRQPSTITMISDW
                     LAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADILSPVKETGTLLGVVS
                     SQAAELCGLKAGTPVVVGGGDMQLGCLGLGVVRPAQTAVLGGTFTDNKQCTLSEARHQ
                     LPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="K0W59_003557"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="K0W59_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HBI3024441.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="K0W59_003558"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="K0W59_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HBI3024442.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAGTERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAEDNATPERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="K0W59_003559"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="K0W59_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HBI3024443.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="K0W59_003560"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="K0W59_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HBI3024444.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWFWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="K0W59_003561"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="K0W59_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..7965
                     /gene="lsrF"
                     /locus_tag="K0W59_003562"
     CDS             7090..7965
                     /gene="lsrF"
                     /locus_tag="K0W59_003562"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HBI3024445.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSWIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            7989..8279
                     /gene="lsrG"
                     /locus_tag="K0W59_003563"
     CDS             7989..8279
                     /gene="lsrG"
                     /locus_tag="K0W59_003563"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HBI3024446.1"
                     /translation="MHVTLVEINVHEDKVEEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8318..9076
                     /gene="tam"
                     /locus_tag="K0W59_003564"
     CDS             8318..9076
                     /gene="tam"
                     /locus_tag="K0W59_003564"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HBI3024447.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9080..9994)
                     /locus_tag="K0W59_003565"
     CDS             complement(9080..9994)
                     /locus_tag="K0W59_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HBI3024448.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTYISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT         2465 a         2521 c         2684 g         2545 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgcagtaaca ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcatg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatggctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt caagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcggcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgttctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 ggaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcgg aagataatgc caccccggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tagaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgc ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atccccaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggt tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gcattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgctggattg gggaatgcag
     7201 tcacgcttat cgtggatatt taatccgaaa acgggtaaaa ctgtgatgct ggcctttgac
     7261 catggttatt ttcaggggcc gactaccgga cttgaacgca ttgatataaa tatcgccccg
     7321 ctgtttgaac atgccgatgt attaatgtgt acgcgcggca ttttgcgcag cgtagttccc
     7381 ccggcgacca ataagccggt ggtactgcgg gcgtcaggtg cgaactctat tctggcggaa
     7441 ttaagtaatg aagccgtggc gttatcgatg gatgatgccg tgcgcctgaa cagttgcgcg
     7501 gtggcggcgc aggtttatat cggcagcgaa tatgaacatc agtcgatcaa aaatattatt
     7561 cagctggtcg atgccggaat gaaagtggga atgccgacca tggccgtgac tggcgtgggc
     7621 aaagatatgg tgcgcgatca gcgttatttc tcgctcgcga ctcgaatcgc cgctgaaatg
     7681 ggggcgcaaa ttatcaaaac ctattatgtc gaaaaaggtt ttgaacggat tgttgccgga
     7741 tgtccggtac ccattgttat tgctggcggt aaaaaattac cggagcgcga ggcgctggaa
     7801 atgtgctggc aggctatcga tcagggcgct tctggtgtgg atatggggcg taatattttc
     7861 cagtctgacc atccggtggc gatgatgaaa gccgtacagg cggtggttca ccataacgaa
     7921 acggctgatc gggcatatga actctatctg agtgaaaaac agtaactgcg gatttaagga
     7981 gaagaattat gcacgtcaca ctggttgaaa ttaacgttca tgaagacaag gttgaagagt
     8041 ttatcgaagt ttttcgccag aaccacctgg gctctgtaca ggaagaaggt aatttgcgct
     8101 tcgatgtctt acaggacccg gaagtgaatt cgcgctttta tatttacgaa gcctataaag
     8161 atgaagacac agtggcattc cataaaacca cgcctcacta caaaacctgt gtcgcgaaac
     8221 tggaatcttt aatgaccggg ccgcgtaaaa aacgtctgtt caatgggttg atgccgtgag
     8281 gctacaattg gggtaacgcg ctgacgggag taaaaaaatg tctgactgga acccctcttt
     8341 atatctgcac ttttccgctg aacgatcgcg tccggcggtg gagctgcttg ccagagtgcc
     8401 gctggagaat gtcgaatatg ttgccgatct tggctgtggc ccaggtaaca gcaccgccct
     8461 gctgcatcaa cgttggcctg cggccaggat aacaggcatc gactcgtctc cggcgatgat
     8521 tgctgaagcg cgcagtgctt tgccagactg ccagttcgtg gaagcagaca tccgcaactg
     8581 gcaaccggaa caggcactcg atctgatttt tgctaatgcc tcactgcaat ggctgcccga
     8641 ccactacgaa ttgtttcctc acttggtttc tttacttaat ccgcagggcg tgctggcagt
     8701 acagatgcca gataactggc tggagccgac ccatgtgctc atgcgcgaag ttgcttggga
     8761 acaaaactac ccggatcgcg ggcgtgagcc gttggctggc gttcatgctt actacgatat
     8821 tttgagcgaa gccggatgtg aggtcgatat ctggcgaacc acttactatc accagatgcc
     8881 gtcgcaccag gcgattatcg attgggtgac cgccactgga ttacgtccgt ggttacagga
     8941 tctgaccgag agcgaacagc agctttttct tacgcgctac catcagatgc tggaagagca
     9001 gtatccactg caagagaacg gacagatact gctggcattt ccgcgtctgt ttattgttgc
     9061 ccgccgtacg gagtaattat cacgtcagct ggtaatgacg atcgggaaga atttttgctg
     9121 gaatttctgc ttcatcgttc atctgtaaca ggtcaatttc aatagcgttg cagatggcat
     9181 ccagcggtaa atcattgttt tcagtaccga acggatcttc cagttcttcc gccagacaat
     9241 ccagcgaaat ataagtgtag gaaatcagca cagagataaa aggcgtcatg taatgcaggt
     9301 ccacgaccag cgcgaacggc agcatgatac aaaacagata aacggtacga tgcaaaatca
     9361 gggtgtaggc aaagggaatt ggcgtgtagg caatgcgctc acagcctgcc agaaccgctg
     9421 aaatatcatt aagccgatcg ttgaggctaa taaacaggat atctgaaagc tgtccattgc
     9481 ggcgctgaac cgccaaccat tctcccatta ttaacaagat acggttagct ggagagttcg
     9541 aagccagtac acgctgaaga tctccagtct tgagataatg agccagcact tccgcctgtg
     9601 gctgtttgcg taatgtcatg cgtaaacagt gggcgaaggc gatttgcagc ctggcaaact
     9661 cccttacact tgccgaatcc ggcaatgtcg tttttacctc tcgcagtaac gaccgtgagg
     9721 caatcatcaa ctgcccccaa agttttcgcg cttcaacgta acgggcgtac ccggcattat
     9781 tacgaaaacc aagaaaaatg gcgatggcga caccgagaat gctgaacggt gcgagggtga
     9841 atttgatgcc cagatgcgtg taccagggca gcatgaatat aacagcaata gaaaagagaa
     9901 aattgagtag taagcgcgag gatatcttgg ataatactga gccgtgccag acaaaaatac
     9961 ggcgcagcca gtgttgttgt ggacgaacaa tcatggttat cttcaggcgt ggaaaagtcg
    10021 ccctattaaa cgtgattacg atcacattct caagacgcta cttacaaatt acctagaacc
    10081 acggcagcct gacggctgca aattgaaaac cgcccctgtt cgtcctgttg ttgccgcacg
    10141 ctccgcagaa tatgtatgct ctgaggacct gttttattaa ggactcatcc tcttatgttg
    10201 acccgcaaaa gtata
//
LOCUS       DAETPZ010000120        10207 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.121, whole genome shotgun sequence.
ACCESSION   DAETPZ010000120 DAETPZ010000000
VERSION     DAETPZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10207
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.121"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            31..834
                     /gene="hpaI"
                     /locus_tag="K0W59_003566"
     CDS             31..834
                     /gene="hpaI"
                     /locus_tag="K0W59_003566"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HBI3024449.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALTARFGNSSSISMAKQNNNSVY"
     gene            856..2211
                     /gene="hpaX"
                     /locus_tag="K0W59_003567"
     CDS             856..2211
                     /gene="hpaX"
                     /locus_tag="K0W59_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HBI3024450.1"
                     /translation="MTTSSLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSGIIMASMGSFTAMTIFWTTQDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            2221..3111
                     /gene="hpaA"
                     /locus_tag="K0W59_003568"
     CDS             2221..3111
                     /gene="hpaA"
                     /locus_tag="K0W59_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HBI3024451.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFTRLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSPEGMEHTLAALNHYWPIISLEFQQNKPG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            3138..4075
                     /locus_tag="K0W59_003569"
                     /pseudo
     CDS             3138..4075
                     /locus_tag="K0W59_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            4302..5860
                     /gene="hpaB"
                     /locus_tag="K0W59_003570"
                     /pseudo
     CDS             4302..5860
                     /gene="hpaB"
                     /locus_tag="K0W59_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            5878..6390
                     /locus_tag="K0W59_003571"
     CDS             5878..6390
                     /locus_tag="K0W59_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HBI3024452.1"
                     /translation="MQLDEQRLRFHDAMVSLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGESRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            6783..8933
                     /gene="btsT"
                     /locus_tag="K0W59_003572"
     CDS             6783..8933
                     /gene="btsT"
                     /locus_tag="K0W59_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HBI3024453.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            8983..9186
                     /locus_tag="K0W59_003573"
     CDS             8983..9186
                     /locus_tag="K0W59_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBI3024454.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            9197..10153
                     /gene="yjiA"
                     /locus_tag="K0W59_003574"
     CDS             9197..10153
                     /gene="yjiA"
                     /locus_tag="K0W59_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HBI3024455.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
BASE COUNT         2429 a         2656 c         2644 g         2478 t
ORIGIN      
        1 ttagctgccg ctttgtttaa ggagagaacg atggaaaaca gttttaaagc ggcgctgaaa
       61 gcaggccgtc cgcagattgg attatggctg gggctgagca gcagctacag cgcggagtta
      121 ctggccggac caggattcga ctggttattg atcgacggtg agcacgcgcc gaataacgtg
      181 caaaccgtgc tcacccaact acaggcgatt gcgccctacc ccagccagcc agtggtacgc
      241 ccatcgtgga acgatccggt gcaaatcaaa caactgctgg acgtaggcac acaaacctta
      301 ctggtgccga tggtacaaaa cgccgacgaa gcccgtaaag cggtacgcgc cacccgttat
      361 ccccccgccg gtattcgcgg tgtgggcagt gcgctggctc gcgcctcgcg ctggaatcgc
      421 attcctgatt acctgcaaaa agccaacgat caaatgtgcg tgctggtgca gatcgaaacg
      481 cgtgaggcaa tgaagaactt accgcagatt ctggacgtgg aaggggtcga cggcgtgttt
      541 atcggcccgg cggatctgag cgccgatatg ggttatgccg gtaatccgca gcacccggaa
      601 gtacaggccg ccattgagca ggcgatcgtg cagatccgcg aagcgggcaa agcgccgggg
      661 atcctgatcg ccaatgagca actggcaaaa cgctatctgg aactgggcgc gctgtttgtc
      721 gccgtcggcg ttgacaccac cctgctcgcc cgcgccgccg aagcgctgac agcacggttt
      781 ggtaatagtt cttctatttc gatggcgaag caaaacaaca actctgtcta ctaaacgtac
      841 gatgtggagc gcatcatgac gacctctagc ctgcaatata aagataataa aactgttgaa
      901 attgaacatc gtgtcattaa taaattattt cgtcgcctaa tcatttttct ttttattctg
      961 tttgtttttt cgtttcttga tcgcatcaat ataggatttg ccggactgac aatgggcaag
     1021 gatcttggcc tgacgtccac aatgtttggc ctcgccgcga cactctttta tgtcacgtac
     1081 gtcatgtgcg ggatccccag taatatgatg ttagcgaaaa ttggcgctcg tcgatggatc
     1141 gctgggatca tggtggtctg gggaattgct tccacctgca caatgtttgc aacaagcccc
     1201 gaaacactct atctactgcg tatgctggtt ggtattgctg aggcgggttt tttacctggt
     1261 attttggttt atctgacctg gtggttcccg gcatatcatc gggccagagc caatgcctta
     1321 tttatgatag cgatgccagt cactatgatg ctgggattga tcctttcagg ctacattctg
     1381 gccttggatg gactgtggaa tctaaaaggc tggcaatggc tctttttgct ggaaggtctg
     1441 ccatcggtca ttcttggtgt cgtcacctgg ttttatctca acgacacacc agaccaggcg
     1501 acctggcttg atgaaaacga aaaacaggct ttaaaaacga tgattctccg tgaacaagag
     1561 agtgcatttg cccatgcatc tgttccacgc tcaaccttac gtgaagtcct gaccccagcc
     1621 atcatgctat atacacttgc ttatttttgc ctgactaata ctctgagtgc gattaatatc
     1681 tggacgccgc aaatcctaca gagtttcaat actggcagta gcaacatcat gattggtttg
     1741 ctggcggcca tcccccaatt ctgcacaatt tttggcatga tctggtggag tcgtcgttcc
     1801 gaccgtctta aagaacgcaa aaagcatacg attcttccct acctgtttgc cgctgccgga
     1861 tggttactcg cttctgcgac agatcacagc ctcattcaac tgagcggcat cattatggcc
     1921 tcaatgggat catttaccgc gatgactatc ttttggacaa cacaggatca agttataagc
     1981 ttgcaatcgc gtgccgttgc gatggctgtc atcaatgctg tcggcaatgt aggttcagca
     2041 gtcagtccat tacttatcgg tattttgaaa gatgcaaccg gcagttttag ctcaggtttg
     2101 tggtttgtag caggattgct ggtactgggc gcgttagtca tgacccgaat ccctatgggg
     2161 agtcgggaac actctctttc atctgaactt atcgcaccaa aaccacaata aggagtcatg
     2221 atgtccagtg aagctatctt tgaacatggt tatgaaaata ttgatttggt ccgggaatat
     2281 gatgcccgct acgctggtga agatgtgcat tatgaaacat ttacccggtt agcgaatttc
     2341 tttggacgcg atatgcgccc tcactggcac gatagatatt ttcaactcca ttatctggtc
     2401 actggacgca ttactttaca actggatgag cacttctatt ctgtgcgcgc accgcttttt
     2461 gtactaacac caccatcggt gccacatact ttctttactg atcctgatag cgatgggcat
     2521 gttctgaccg tacgtcagga gttaatttgg cccctggtag aaaagctctg gcctggtagt
     2581 ggagatgccg caatgctaca aggtatctgt ctgtcaccgg agggaatgga acatacactg
     2641 gcggcattaa atcattattg gccgataata tccctggagt ttcaacaaaa taagccagga
     2701 cgggaaattc tattgcaatc actggcgcaa agtattttta caatcctact gcgtgaagca
     2761 ccaccaaatg atatttcaac atgcagcgtg cgcggcgaga tgtgtttatt ccagaagttt
     2821 aatcggctga ttgacgaaaa ttatcgccaa catttaatgg tccctgaata tgcaacaatg
     2881 ctcggtatga gtgaatcacg gcttaccgag ctgtgccgac gttttgccaa tcaatcgccg
     2941 aagagattga ttttcgaacg agtgagccgg gaagccagac ggcttctgct ctatagcgag
     3001 caaagcatca accagatagc ccttgatctt ggctacaaag accccgccta tttcgcccgt
     3061 tttttcaacc gaatgatggg ttgctcacca acacaattcc gtggtcgctg accatttatc
     3121 aacctgagag actcgacatg aaaatatctt tacgctcact atttgtggcg tgcctgattg
     3181 cgccagtggc attcacctac gcctccgaac cgcaataaat gaaaattgta ctacttggaa
     3241 cgaaaggtgg cccttcttta ttaaaagctg accgtttacc gatggcaacg gcacttatta
     3301 ttggcaataa aacctggtta attgatgctg gttacggggc cagcttacaa ctggtaaaaa
     3361 ataatattcc actgcgccag attgataata ttttactcac ccatttacac agtgatcata
     3421 ttctggacta tccatcagtc ttaatgaatg catgggctag cggtctgcag gacaaaacta
     3481 ttcatgtatt cggtccgcca ggcacccgag ccatgaccgc tgccagttgg caggtcttcg
     3541 atcgggacat cacactgcgt atggaagaag aaggaaaacc cgatcctcgc aaactcgtta
     3601 cagctcaaga tgttggtgaa ggcgtagttt ataaagatga taccgtgacc gtgactgcat
     3661 taaaggtccc tcattctcct ttccctgacg gtgaggcttt tgcttatcgc ttcgacacac
     3721 aaggcaagcg aattgtcttc tcaggagata cctcgtactt tcccccactg gcaactttct
     3781 cagaaggcgc tgatatactt gtgcatgagg ccgtacatga gccctccgtc gctaagctgg
     3841 cagacagtat tggcaatggt aaaacactgg caaaagccat tgcttctcac catacgacta
     3901 ttgaggatgt aggcaaaatt gccaatgaag caaaagtgaa aaaactcatc cttagccacc
     3961 tggtaccaat caccgttccg gatgaaacat ggaagcaaga agcacagaaa actattcagg
     4021 ccctatcgtc gttggtcatg aaaatatgac gattaccatt ccattcgccc cataaaaaat
     4081 tccgtccccg ttagcccttg ttagcgggga atattatgat ttgccttact gtaagttgat
     4141 cacgatcgca gtttactatt taactccacc ttgctgtcga aaagtaccaa cgttcgcctg
     4201 aaccgtctct tcccgacaac cgcgttcaac gcttcaatta aataacaaaa acaacacaca
     4261 aaattaacaa tcgtattccg attaatactg tagaggtcga catgaaacca gaagatttcc
     4321 gcgccagtac ccaacgtcct ttcaccgggg aagagtatct gaaaagcctg caggatggtc
     4381 gcgagatcta tatctatggc gagcgagtga aagacgtcac cactcatccg gcatttcgta
     4441 atgcggcagc gtctgttgcc caactgtacg acgcgctgca caaaccggag atgcaggact
     4501 ctctgtgttg gaacaccgac accggcagcg gcggctatac ccataaattc ttccgcgtgg
     4561 cgaaaagtgc cgacgacctg cgccagcaac gcgacgccat cgctgaatgg tcacgcctga
     4621 gctatggctg gatgggccgt accccagact acaaagccgc tttcggttgc gcactgggcg
     4681 cgaatccggg cttttacggt cagttcgagc agaacgcccg taactggtat acccgtattc
     4741 aggaaactgg cctctacttt aaccacgcga ttgttaaccc accgatcgtc atttgccgac
     4801 cgataaagtg aaagacgttt acatcaagct ggaaaaagag actgacgccg ggattatcgt
     4861 cagcggtgcg aaagtggttg ccaccaactc ggcgctgact cactacaaca tgattggctt
     4921 cggctcggca caagtgatgg gcgaaaaccc ggacttcgca ctgatgttcg ttgcgccaat
     4981 ggatgccgat ggcgtgaaat taatctcccg cgcctcttat gagatggtcg cgggtgctac
     5041 cggctcgcca tacgactacc cgctctccag ccgcttcgat gagaacgatg cgattctggt
     5101 gatggataac gtactgatcc catgggaaaa cgtgctgatc taccgcgatt ttgatcgctg
     5161 ccgtcgctgg acgatggaag gcggtttcgc ccgtatgtat ccgctgcaag cctgtgtgcg
     5221 cctggcagtg aaactcgact tcattacggc actgctgaaa aaatcactcg aatgtaccgg
     5281 caccctggag ttccgtggtg tgcaggccga tctcggtgaa gtggtggcgt ggcgcaacac
     5341 cttctgggca ttgagtgact cgatgtgttc agaagcgacg ccgtgggtca acggggctta
     5401 tttaccggat catgccgcac tgcaaaccta tcgcgtactg gcaccaatgg cctacgcgaa
     5461 gatcaaaaac attatagaac gcaacgttac cagtggcctg atctacctcc cttccagtgc
     5521 ccgtgacctg aacaatccgc agatcgacca gtatctggcg aagtatgtgc gcggttcgaa
     5581 cggtatggat cacgtccagc gcatcaagat cctcaaactg atgtgggacg ccattggcag
     5641 cgaatttggt ggtcgtcacg agctgtatga aatcaactac tccggtagcc aggatgagat
     5701 tcgcctgcaa tgtctgcgcc aggcacaaag ctccggcaat atggacaaga tgatggcgat
     5761 ggttgatcgc tgcctgtcgg aatacgacca gaacggctgg actgtgccgc acctgcacaa
     5821 caacgacgat atcaacatgc tggataagct gctgaaataa cgcagcagga ggttaagatg
     5881 caattagatg aacaacgcct gcgctttcat gacgcgatgg tcagcctgtc ggcagcggta
     5941 aatattatca ccaccgaggg cgacgccgga caatgcggga ttacggcaac ggctgtctgc
     6001 tcggtcacgg atacaccacc gtcgctgatg gtgtgcatta acgccaacag tgcgatgaac
     6061 ccggtttttc agggcaacgg caagttgtgc gtcaacgtcc tcaaccatga gcaggaactg
     6121 atggcgcgcc acttcgcggg catgacaggc atggcgatgg aagagcgttt tagcctctca
     6181 tgctggcaaa aaggtccgct ggcgcagccg gtgctaaaag gttcgctggc cagtcttgaa
     6241 ggagagagcc gcgatgtgca ggcgattggc acacatctgg tgtatctggt ggagattaaa
     6301 aacatcatcc tcagtgcaga aggtcacgga cttatttact ttaaacgccg tttccatccg
     6361 gtgatgctgg aaatggaagc tgcgatttaa gctccagccc cttcacgcat tcgtcgaagg
     6421 ggcattttta ttgtgatctc aatcacatat ctacccgatg aataattctt gcccaaccgc
     6481 tcaccgccat tcctgaccgc ttacatccct aaaataacca ctcagttatt taccttactt
     6541 tacgcgcacg tatctctggc aacatcactg caggatagcg gtcaatttac ctcctcaaac
     6601 gcaacgcaaa cctagaacgg cttcggccta tgattaacaa attactctat ggattttggt
     6661 ttgcaggaag gcagcaagtg agtgaatccc cgggagctta caaaagtaag tgtcagaggt
     6721 gaacgaacgc agctgccgca cctgtaagcc aaaagacgac gagtaaaatg ccaggtttta
     6781 ctatggatac gaaaaaacta ttcaagcaca taccctgggt gattctcgga atcatcggtg
     6841 cattctgcct cgcggtagtt gcattacgtc ggggggagca cgtcagcgcc ctgtggatcg
     6901 tggtcgcctc tgtgtcggtg tatctggtgg cgtatcgcta ctacagtctg tacatcgccc
     6961 agaaggtgat gaaactcgac cccacgcgcg cgacgcctgc ggttattaac aacgacggtc
     7021 tgaactacgt tccgaccaac cgttacgtgt tgtttggtca ccacttcgcc gctatcgccg
     7081 gtgctggtcc gctggtggga ccggttctcg ccgcgcagat gggctacctg cctggcacgc
     7141 tgtggctgct ggcgggggta gtgctggccg gtgcggtaca ggactttatg gtgctgttta
     7201 tctcctctcg ccgtaacggc gcatctctcg gtgagatgat caaagaagag atggggccag
     7261 taccggggac tatcgcgctg tttggctgtt tcttaatcat gatcatcatc ctcgccgttc
     7321 tggcgctgat tgtggttaaa gccctggccg aaagtccgtg gggtgtcttc accgtttgct
     7381 caaccgtacc gattgcgctg tttatgggta tctacatgcg ctttatccgt ccggggcgtg
     7441 tgggtgaagt ctctgttatt ggtatcgtgc tgctggttgc ctctatctac ttcggtggcg
     7501 tgattgctca cgatccgtac tggggtccgg cactgacctt taaagacacc accattacct
     7561 tcgcgctgat tggctatgcg tttgtttccg cactgctgcc ggtgtggctg atcctcgcac
     7621 cgcgcgacta tctggcaacc ttcctgaaaa tcggcgttat cgtcggcctg gcgctgggta
     7681 tcgtggtgct gaacccggaa ctgaaaatgc ctgcgatgac ccagtacatt gacggtactg
     7741 gcccgctgtg gaaaggcgct ctgttcccgt tcctgttcat caccatcgcc tgtggtgcgg
     7801 tatctggctt ccacgcgctg atctcttccg gtacgacgcc gaaactgctg gctaacgaaa
     7861 ccgacgcgcg tttcattggc tacggcgcaa tgctgatgga gtccttcgtg gcgattatgg
     7921 cgctggttgc agcgtccatc atcgaaccgg gtctttactt cgcgatgaac accccgcctg
     7981 ctggccttgg catcaccatg cctaacctgc atgaaatggg tggcgagaac gcgccgatca
     8041 tcatggcgca gctgaaagac gttaccgcac acgcggcagc gaccgtcagc tcctggggct
     8101 tcgtgatttc gccagagcag atcctgcaaa ccgcgaaaga cattggtgag ccttctgtcc
     8161 tgaaccgtgc aggtggtgcg ccgacgctgg cggtaggtat cgctcacgtg ttccacaaag
     8221 tgctgccgat ggctgacatg ggcttctggt atcacttcgg tattctgttc gaagccctgt
     8281 tcatcctgac cgcgctggat gcgggtaccc gttctggccg ctttatgctg caagacctgc
     8341 tgggtaactt catcccgttc ctgaaaaaaa ccgactctct ggttgccggt atcatcggta
     8401 ctgcgggctg tgtgggtctg tggggctacc tgctgtatca gggcgtggtc gatccgctgg
     8461 gcggcgttaa gagcctgtgg ccgctgttcg gtatctccaa ccagatgctg gcagccgtag
     8521 cgctggtact gggcaccgtt gtgctgatta agatgaagcg cacccaatac atctgggtaa
     8581 ctgttgttcc ggctgtatgg ctgcttatct gcaccacctg ggcgctgggt ctgaaactgt
     8641 tcagcaccaa cccgcagatg gaaggcttct tctacatggc aagccagtac aaagagaaga
     8701 ttgctaacgg tactgacctg acggcgcagc agattgccaa catgaaccac atcgttgtga
     8761 acaactacac caacgcaggc ctgagtattc tgttcctgat tgtggtgtac agcatcatct
     8821 tctacggttt caaaacctgg cttgcggtgc gtaacagcga caaacgtact gacaaagaaa
     8881 caccgtacgt tccaatcccg gaaggcggcg tgaagatctc ttcgcaccac taaccgtgtt
     8941 tagccccgct tcggcggggc tttgttctta tcagagtgaa ctatgtttgg taacttagga
     9001 caggcaaaaa aatatctcgg ccaggcggcg aagatgttga ttggtattcc agactacgac
     9061 aactacgtcg agcatatgaa gaccaaccat cccgacaagc cgtacatgag ctatgaagaa
     9121 ttcttccgcg aacgccagaa cgcgcgctac ggcggcgatg gtaaaggcgg tatgcgctgt
     9181 tgttaatgga gagagcatga acccgattgc agttacccta ctcaccggtt ttttaggcgc
     9241 gggcaaaact accctgctgc gccatattct taacgaacaa cacggctaca agattgccgt
     9301 gattgaaaac gaattcggcg aagtctccgt tgatgatcaa ctgattggcg accgcgccac
     9361 gcagatcaaa acgctgacca acggctgcat ctgctgttcg cgctccaacg agctggagga
     9421 cgcactactc gacctgctgg ataatctcga caagggcaat attcagttcg atcgtctggt
     9481 gattgaatgc accggcatgg ccgatcccgg cccgattatt cagacctttt tctcccatga
     9541 gattttatgc cagcgctacc tgctggacgg cgtgattgcg ctggtggatg cggtacatgc
     9601 cgatgagcag atgaaccagt tcaccatcgc ccagtcccag gtgggctacg ccgaccgtat
     9661 tctgctgact aaaaccgacg tcgcaggcga agcagagaaa ctgcgtgaac gcctggcgcg
     9721 catcaacgcc cgcgcaccgg tctacaccgt cacccacggc gacatcgacc tgggcctgct
     9781 gttcaacacc aacggtttta tgctggaaga gaacgtcgtc agcaccaaac cgcgtttcca
     9841 ctttatcgcg gataaacaaa acgatatttc gtcaattgtg gtggaactgg attacccggt
     9901 agatatcagc gaagtttccc gcgtgatgga aaacctgctg ctggaatcgg cggataaact
     9961 gctgcgttac aaagggatgc tgtggattga cggcgaacct aaccgcctgc tgttccaggg
    10021 cgtccagcgc ctctacagcg ccgactggga caggccgtgg ggcgatgaaa aaccgcatag
    10081 cacaatggtg tttatcggga ttcagttgcc ggaggaagag attcgggcgg cgtttgcggg
    10141 attgaggaag taagttcggg aaagatcaac tttttgagag ttccaccgca acattattac
    10201 gagtgga
//
LOCUS       DAETPZ010000121        10109 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.122, whole genome shotgun sequence.
ACCESSION   DAETPZ010000121 DAETPZ010000000
VERSION     DAETPZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.122"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..272)
                     /locus_tag="K0W59_003575"
     CDS             complement(<1..272)
                     /locus_tag="K0W59_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HBI3024456.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVWC"
     gene            complement(366..928)
                     /locus_tag="K0W59_003576"
                     /pseudo
     CDS             complement(366..928)
                     /locus_tag="K0W59_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1132..1338)
                     /locus_tag="K0W59_003577"
     CDS             complement(1132..1338)
                     /locus_tag="K0W59_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HBI3024457.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1445..2008)
                     /locus_tag="K0W59_003578"
     CDS             complement(1445..2008)
                     /locus_tag="K0W59_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HBI3024458.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3383..5218)
                     /locus_tag="K0W59_003579"
     CDS             complement(3383..5218)
                     /locus_tag="K0W59_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024459.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5319..5606
                     /locus_tag="K0W59_003580"
     CDS             5319..5606
                     /locus_tag="K0W59_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBI3024460.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5578..7107
                     /locus_tag="K0W59_003581"
     CDS             5578..7107
                     /locus_tag="K0W59_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HBI3024461.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7277..8494)
                     /locus_tag="K0W59_003582"
     CDS             complement(7277..8494)
                     /locus_tag="K0W59_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBI3024462.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8861..9847)
                     /locus_tag="K0W59_003583"
     CDS             complement(8861..9847)
                     /locus_tag="K0W59_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024463.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFLWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
BASE COUNT         3179 a         2304 c         1877 g         2749 t
ORIGIN      
        1 ttacaccaga catcgttatt aagtagcagc accataagat aaggagtggc atcgttagcc
       61 acagcttccc tactccagag gtaatataaa ggggtgggct cgactgattt atctttacgt
      121 cgcttacact gcaaatgttc agaaattagt ctatgcagct caccagtaaa atcagcaatc
      181 agagaaggaa ttgccttatt cataccaggg caaggtatta atttaaactg taataattta
      241 attttaggat gtgtagcagc agcccgatac agagttgcaa ggacacactt ttgccagagg
      301 gcgttacagg aaagcttaat gtttgattct gtatacataa taaatcacct tatagttaca
      361 ataggtcaaa aaccgctgta gccaaagtta cgctggcctg atgctttagt gtcgagcttc
      421 gtcaaataat ccagacgtac caataagcgc tggtactgct ctgggaaatt aggatcgtta
      481 atatccagga tgtaacgtcc attagcaggg aaatgaacta atccctggtg atcttccagt
      541 tccaacccta aggcactgta ccaggctccg gtaattaacc ctctcagggt gtcagagcgc
      601 tcatagtttc ccagatggta ataggcgtct ttgttaaaaa aaggcagacg tgataatgac
      661 atttaaagga ctgcgtatat tctttcgccc agataataca aaccgacgtt gcgtaggtgc
      721 gtttgccttg ttgtttttta cggtgttgat ctgcattaat tttcgcattc agcgagttac
      781 ggaaacgaga tatctctccg cggatcaggt tagggaaaca ggtaatgata tccccgttgt
      841 caatcacagg agggtaatgg agatcccccc gtattgctgt tattctggaa gtgtttttaa
      901 tattcgtatt gatagttctc agagatatat cacactctag atattttaat taagtatcag
      961 ttcgatatcg gcatattgta cactacctca ggtagatgat gagatcagat acagggatca
     1021 actgacggtg agcgcagtcc ccacatttta tccagggctt actgcatatt cccaacagcc
     1081 attcgttagc caggtagacg aataccctga tttgccgctg gttttgcttt cctatcgaag
     1141 ggcccataca tcatcacgcc cacgaaacaa ccgacgaaat aacgcaactt tctcatccgt
     1201 ggataatacg aaaacgcact gcacagggct ctgcggttta cgtcgccatt caatcccatg
     1261 agcttcaagt aatgagacca gccgggtatt ttctgcccgc agtactgcta gttcatcgct
     1321 gtcagattta taaaccatat gcgttatcac ttgtacctgt ctcccggcgg caccacgact
     1381 gaaattcgtg tgaaggcaaa tccacgcgat aacttacgat agttcggaca taagccccca
     1441 taattcagtt atgtcgtggt cgaccccggt tacttttttc gggaagcctg ctacggccca
     1501 gaataatacc gtgttctttc tgatcctgtt tagccagata gctaagggcg taacgtaagt
     1561 cgtctaccgc agatttatca ctatagtgaa tcacgtgatc gatacgcacc gggtatttgt
     1621 ctttagcccg gcacagatga aaataaccct ccccttccgt gatccgcctc cagatatctc
     1681 ccagttgcct ggatatctga taagagttct tgtgacgttg tccatccagg tagccgataa
     1741 aatgaatatg aagccctttg tctggtgtat attccattac ccagtaatat cccgccaaca
     1801 tcgtctgggt ttcgctaagt aaacggtata tttccataca catactgtgc ttacaggaat
     1861 gcccgaaact gggcgtgtct ttcctgtaag caaaatcaat tctgaaaggt aacagtttag
     1921 aaaaacgttg aaacatacca tccatgtgtt cattcacgtc tttcagaatc atgaaatcca
     1981 ttttgtaatt gggattggca ttatacattt tatagtcctt actttataaa actcacagga
     2041 agagagaatt acgtaaatac tgagagtaga gaaacgccgt accacggcat taataaacga
     2101 agtaaaccag cagcacagac aacctgataa aactgttttg tattaccgca ttactgtgat
     2161 gaggtaatac ttcagaatac agtgagactc tcttaaactg agaaccagta cgaattacaa
     2221 tggaaaaaca cacctcatag aattgagttt caggaagcac cactaaattc tttacaggaa
     2281 gtaatctgac attcacaaaa ctcagtatcg gctagctaca ggcaacatcg ctattaatat
     2341 tgtcgaacca ttaagtaaag ccgagataga ccacgggtat ttaatcatta gcataaaaga
     2401 ataatggtat acagaacata cccacacacc ataagaagat tgttccatac agaaaagatc
     2461 atatttacct atacctataa actgtaacgg agtcataaca cgctctaata gtgttccgat
     2521 acgtaaccag tgttaagtac gtacaggaca acattacaca cataattcac catacagctt
     2581 cccccatatg cttcactgcg tagtgtatgg agatatatgc attacacaca tacagaatac
     2641 ttagcataaa gaggagtgac attaccacgt aaagagataa gttatacaac aaacatacaa
     2701 acaatacggc acagacacac aataccataa ctccaactaa taatactaat taaccttaat
     2761 tatttatggt atatgaaata aatgtgattc tcccggaact aactaaccta accagacaga
     2821 tgatcactgt ataaaaactt cagttaatac tcatttgttt tgtcgactca gtatataatg
     2881 agtgaccagt aatcaggata atcacactta cataacctgt aaaaaagaac aagaagaata
     2941 agccactaac ataatgcaaa atggcatctg aatcgatcgc agtttggcat tcgaatcgat
     3001 catgatttgg cccaatccgt gatcgaatag agcggaatac gcaacataat tcaccagaca
     3061 agagcttaca ccgaattaca gattatgtat taatgtcaaa actggcatat tttacagata
     3121 caatgaacat gcaacccatc aatctaatag gggcggtatg tacatctgcc catgaatggg
     3181 atggcgctaa gaatacaaaa caattagaca tagatcacaa aaacacacca ttctaattga
     3241 aaaacaaaac aaacaaataa atcatactat tatagaattt tctcttgcca gacatagata
     3301 atacccctgc taattttgct aatggttagc aaaattagca gggtatataa agattattga
     3361 cccaaaacac actcagatga attcacgaca gctcaggagg gttattcctg atgtcatctt
     3421 cacaaataat ggttaacgca tcatatatca ttctgcgtct gttcctgttt tcggccttat
     3481 cgccatacaa catcatcaca tttttaattt catcgaccgg tgatttcaca tagtgaacta
     3541 atacaccaag cttctcaaca cattgcagta aagtatattt cttactgcct gtgaaaagcc
     3601 ctatcatagt ttctttacgt tcgcgactgg ataatacttt gttttccata atgacttctg
     3661 ccagcgtctt accatcataa acatactctt tatctatcag aatatttatc ttatccagca
     3721 ccttctctac gtcatcaagc aaacctgtaa tatatggggg cgcaatatat ggtttcgtac
     3781 tgatcttttc aagcatacaa ttatacatct taataacacg catcagggta aaattgttcg
     3841 catcatactg tatttcaccc tccttatata caggatcaat aagtggtatt ttttcaataa
     3901 atgcatcctg ataggctttt acatggtcaa gccaataaaa caacgagcca tactttatat
     3961 tttttctatt tactttaacc tccagtgctg tccgaagcaa cgataatgcc cccctgacat
     4021 atcctgaagg taattcacca aacaatcctt ttctttccat gtcatcataa tgctgcagca
     4081 catcattaat tttatttttc cttatcagac aaatgtacac cgcaagtgtt tcgtagcagc
     4141 tggtcacatg aacagcctca ttctgcaact gcccaaaata gcttataatc tttttcagat
     4201 atgcgtcatc acaaccattc ttatcattaa tattaaatac aagatctttc agacgggaaa
     4261 tatattcctg agatttatca tcaagtgaaa cactataacg agacaatgtt ccttctgcac
     4321 tgttttgaaa cttataaaac atttccagta atttttttgc ataaggcgtt gtatcaaagc
     4381 tggtaaagtc ataaagatat tgcgcagcgt aaccatacaa accatgaatc cagagtcctt
     4441 ctctcaccaa cactctgaga taatatgcaa ggctttgaag tatcttcgtc tttcgttgta
     4501 tatcaagtaa gacacttaat gaataaaatg cattatagaa tttatcaata ttcctgagca
     4561 ctttctccag ttcatcctta tccagcttct tctcccggta atccttttta tattcatttt
     4621 tgatagccat gatatattgt tgcatcaggc tcttgctttt cccgttatcc ttccgtatag
     4681 ttcctgtata tttccccaga gcatacagct ttccattttc atgaaaatct tccttgttga
     4741 atcccagttc agccagatat tcccgaagat aacgtttaac agcagcttca tcgctttctg
     4801 tatcagcgtg atcaagacaa ggacaatacg atttctgtaa aaaattataa atatgataat
     4861 aaaaagcaga gtcattctct ggtctgtatg tattatacat atctacaaca agaaaaataa
     4921 ccccaaaagt gcgcagacaa tagctgcgaa ggaattcagg ttttagtgtg gaatgcactg
     4981 gataagaatt catcatgtaa taaaaatgtt catatcgggc atatatgatc cgccgttgct
     5041 cctgactgaa caccccttca gcatcaataa tacgactgat ctcattataa cgttgatccc
     5101 agaatttttg atgcatttca aaattggtac tcttccgctt gtgtgccgga ttctcgcctt
     5161 ccttaaacaa ccccaaaaca gtacaaatat catacaacat atttgtaaat gtgcgcatat
     5221 gctgtgctct ccggttggtt tgcattttat cgcagaggac attctgagct taacgtatca
     5281 aactgacaca acgacatgca atgcatatgg agaacaatat gtcaaatcaa accggacagc
     5341 tttacacgcc ttcacgcatc ctgcgtatgg ctgaacttac ccgactgctg ggtatatcaa
     5401 gatctactat ttacgtgaaa ataaatccgg cctcgaaata ccacgaccct acctttccca
     5461 aaccaataag gcttggtagt gcatccgtag gctggcgggc atctgccatc gatgagtggc
     5521 tgatgttaca tacggcacca gcctggtctg aacctgaaaa caaactaaag gaacttaatg
     5581 atgacgacaa aactatcact ccctgaactt gacacaacta aactgcctga caggtgccag
     5641 gcattacttg atgaaatgca tgaagaaaca ggaatacgcc gtgaaatact tctgtctgtt
     5701 atgctgacgg tcatggctgc ctcggtccag gatacgcatg aagttgaact ttccggaggg
     5761 caacgtacca gccttcagat atttatgtgt ctttcatcag catcaggcag tggaaaaacc
     5821 tctgcctgcg caaaattaat cgctcctgtt catgaaacag aagaagaact acatcaggca
     5881 tatatcgacg ataaaaaaaa ctatgaccga atgatggaaa tgtggacaac agataaaaaa
     5941 atcctggagc ggagatataa aaaggaaatt gaaagatccc cggaaaatgc tgctgctgca
     6001 cgagcagcac ttgaaaagtg tatagcaaat aaacccgaac ctcccgtaca acaggttctc
     6061 atcgtgaatg acgcgacacc agaagggata gctttgaaac tcagccagtc accctctctt
     6121 ctgttgctgt ctgatgaagg aggtactatt ctggataagc gtttcgaacg caaatcggca
     6181 ctgtacaaca cattatggag tgggcaacca gtcaacgttg aacgggcatc cagacccggg
     6241 ttccggctga atgatgtccg tctcactata cttatactga ctcaaccggt aatatttaat
     6301 aagtttttta ctctcactgg cgatcagatc cggggcaatg gctttctggc ccggttgcta
     6361 ttttgcgaac ccggagcaat taaaataatg acgacagaac cgcatactgc cactcctgtt
     6421 gtgacgcaac aatgtgcaag ctgttttcgt aagaaaagtt ttggcagtca gatcagagac
     6481 tctctgagag catccagaga aagacgtgca aaaagtaaag agcgcatctg catgacatta
     6541 tcaaatgcag catcccgcgc actggagcgc tttcacaaag aaaatatgga tactgtcaga
     6601 cagaatcctc atatgggtac atttgaagac attattgtca gaaaaagaga gcaggttgtc
     6661 aggatagcag cacttctgga actggagaaa gatccagact cgacagtaat aacgctggaa
     6721 agtactaata gcgccattta tctcattgaa ttttatttca agcatcttat atataaactt
     6781 gaatcccttc gggaaatatc cccggctgag aaacttgata aatggcttca gaaaagaatt
     6841 atcacaacag caggttacat attccagaaa agttatattt tacaatatgc cccatatgct
     6901 ctcaggaaaa aatgtgttct ggatgaagca cttgacatac tggcggaaca aaggaaaata
     6961 aggattgatg ataacctggt tgtttatata ggaaacacga ttacaccatc cgaactggca
     7021 aaaaaactaa atattcctgc attcgatgcc ggagttttca tttgtgatca tcaaaacata
     7081 ctgaagtatc accgaaacag actatagata ctatcggaca aaaaataaat tatcacaagt
     7141 gtattattcg ttataataat gaataatcgt tatctgagtg caatgccagg ttttcattac
     7201 gaactggcat tgcatttatt tttcaaaaaa gaaagcagta accatttggc tactctgagt
     7261 atgccagtcc atgaaactaa gcgaattcct tagtgttttt agcaagttga gacatcacat
     7321 aatccgccca tgcctgcatc atcggtacac gctgctccag tagatcagta cgatgatatg
     7381 ccgcctcaac cttatttttc agcgtatgag cgagcgccct ttccgccaga tcccgcgaat
     7441 acccctgttc actacaccag tccctgaatg ttgagcgaaa accatgtgcc gtggcaactc
     7501 gccccggaat gtcactgacg gctttctttt tacgcagaaa acttgtcaac accatatcgg
     7561 aaaggatctg ctgctttctg ggtgaaggga acaccagttc atcatgcagg ccacgtatat
     7621 tttccagaat gtaaatagcc tgccgggata aaggaacacg atgctgtagc ctagctttca
     7681 ttctttctgc aggtatagtc catacccgct tatgaaaatc aatttcagcc cagcgcattc
     7741 ccctggcttc gcccgagcga gttgctgtaa gtatcaccat taataacagt gcgcgggtaa
     7801 cattataagg ttcatcacta tacacactgg tcgccacaaa aagcggtaac tgcctccagg
     7861 gcattgcggg ttggtgttca tcacgtcctc ttgtctgctg aggaagcaaa tggtcaacca
     7921 catcaacagg atttgccaca caaaaaccgt gcgcccatcc ccactgcata acaacatgaa
     7981 tgcgctgttt aacccggctt gccgtttctg acaaggttaa ccagactgga cgcagtgttt
     8041 ctgccacgtc cgcagccgta atcgaatcca gcgtttttgc tcccagttga ggaaacgcgt
     8101 aattctcaag cgtcgataac cactgcctta catgctttgg attctcccat ccaggagaca
     8161 gttctgcatg tacacgcctg gctgcatcgg caaatgttgg gataacgact ttctcagatt
     8221 cagccttttt aatctccaga ggatcatcac ctgcagcaag ttgctctcgc attatccggg
     8281 cagtacgtgc agcttcagca atactgacct ctgggtaagt tcccaatcca gcattacgtc
     8341 ttttttgtgt caccggactt acataacgaa aaacccattt cccccgcccc tttactgaag
     8401 aaggatgaag ggtcagtccg gtaattcccc catggggcaa tggtttgtca tcaggtttga
     8461 tatgtcttgc tttcgtatcc gtcaatactg ccatacgcta attcccgtca ctctggtatg
     8521 ccatccagta tgccattagt ccctcgcttc actaagaata cattagataa catcggacaa
     8581 caaaaagtgt aatacattgt ttataaagaa attatataac acccctggat taagtcagat
     8641 ttatttcagg cggtccccct caccgccata tttaaagaag agcgcgtacg aaagtacggg
     8701 cttttttttc gtatattgta cacaccgggt gcccccgccc acgtctgtta gggcgagggg
     8761 aaattgtgca catcaactgt ctggttactc agtcaacaat caaaatgcgc aagatagtac
     8821 agaacatata tgcgttatct ggtcacttcc tgtaacttcc tcacttatta aaatgccccc
     8881 aacggaaata catgtcacat gccatcataa aatcgttatg ccagaggaat cgacaatgga
     8941 aaaaatagta aattaagcac ggtacatagc agagtaaaaa ataggcttca aaccaaatcc
     9001 gccattccac aagcgttgcc aataccgaca ccagcgtaat ccacatcatt tcaataacca
     9061 gcaatatgaa taaccgaagc caaagcggct tggcaaaaaa ggtcgacaac gtattttcac
     9121 tggagaaaaa cagatcgatt tcctgcaaaa aaaacctgcc cagaaaagcg taccgcttgg
     9181 agaagcgcag aaaaaacaga ttaatcgcca acactacgcc acaaagaatg agcaatgcaa
     9241 ttatcgattt aaacgttaac cactgcccct ctgtcacaaa agaatcactg ttacgcaacg
     9301 ggctgatagt aagcaggtta acaaccgttg agatgcacac cgaaagcagc acatagcgag
     9361 tcagactttt atattttact tttttcgcta ctgaaatagc gacctgcttg acataactgg
     9421 tgcggatcat ccgaaacagc agaaagatga ccaacggccc aagtaacgaa atcaggaaca
     9481 gcgcatataa cgagggtaga agctggtata gcccccaact cattgcactc aacatgatta
     9541 acgacagaaa tcctgccacc agaggttgtg taagaaataa ttctattttg tggttctgct
     9601 ttttcttaaa ccacaaaaaa tcgatattct tcgtgacgca tttataagcc cagtacgcct
     9661 tgagatcaaa aataaaatta tacagcacca gggccagaat cccactaact acaccaatat
     9721 cctctgatgg aaaagtaaac cctgcatttt tccaataaaa gcctatcaga ataagtccaa
     9781 tagcataaat gtatatcaga tttcgtttat cctgaaagcg aataaaagtt aaataatcag
     9841 gaatcatttg aatacctttg catcactaaa tgctttacga caatgcataa ctcgttgctg
     9901 atccacgcca gcaaaatcat ttaacatatt ccccaaatag ggcttattac ttatttcaag
     9961 taggcagcct gacggctgcg gcttgtcatg gtctgcaatt accgttataa aaacaggcaa
    10021 tgtcattgtc tttcaggtgg ttatatggcc cgttcagcta aaccccgtaa acgtaaaccc
    10081 gcctctcaaa gaagcaaact tccccgcta
//
LOCUS       DAETPZ010000122        10084 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.123, whole genome shotgun sequence.
ACCESSION   DAETPZ010000122 DAETPZ010000000
VERSION     DAETPZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.123"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..355)
                     /locus_tag="K0W59_003584"
     CDS             complement(<1..355)
                     /locus_tag="K0W59_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI3024464.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIG"
     gene            787..1905
                     /locus_tag="K0W59_003585"
     CDS             787..1905
                     /locus_tag="K0W59_003585"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBI3024465.1"
                     /translation="MPLPDFHFSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1971..2219
                     /locus_tag="K0W59_003586"
     CDS             1971..2219
                     /locus_tag="K0W59_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HBI3024466.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            2284..2652
                     /locus_tag="K0W59_003587"
     CDS             2284..2652
                     /locus_tag="K0W59_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBI3024467.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2746..3399
                     /gene="nfsB"
                     /locus_tag="K0W59_003588"
     CDS             2746..3399
                     /gene="nfsB"
                     /locus_tag="K0W59_003588"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HBI3024468.1"
                     /translation="MNIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3507..4754
                     /gene="ybdG"
                     /locus_tag="K0W59_003589"
     CDS             3507..4754
                     /gene="ybdG"
                     /locus_tag="K0W59_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HBI3024469.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTKAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4822..6198)
                     /gene="pheP"
                     /locus_tag="K0W59_003590"
     CDS             complement(4822..6198)
                     /gene="pheP"
                     /locus_tag="K0W59_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HBI3024470.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGDAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGRNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMTLVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6299..9434)
                     /gene="cusA"
                     /locus_tag="K0W59_003591"
                     /pseudo
     CDS             complement(6299..9434)
                     /gene="cusA"
                     /locus_tag="K0W59_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
     gene            complement(9446..9987)
                     /locus_tag="K0W59_003592"
                     /pseudo
     CDS             complement(9446..9987)
                     /locus_tag="K0W59_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
BASE COUNT         2480 a         2787 c         2430 g         2387 t
ORIGIN      
        1 tgccaatctt gagcgcgcgt aaaccagctt ctccgcgctc ttcatagacc ttcagccacc
       61 tggctacaga accactacca gcaagcataa agtgagcagc agcctgatta agggacatgt
      121 gctgctcgat cacagctttc acgaccttaa tacgcaactc tggatcagca ctaacgcctt
      181 taggtttggg aattaaacct ttttctccat gtttttcata gagggcaacc catgtcctga
      241 cctgggttcg ggggacacca aaacgtgccg agatgatcct gtaaccatca tcagtagtga
      301 agtagtgatt cacgacttca aggcgctttt caaaagggta ttttggcttt gacatattag
      361 gggctattcc atttcatcgt ccaacaaaat gggtgcagta cagggttgat agaaatatct
      421 cctggggctt catctttcca gacctcagag taaacctgaa atcggaaagc ttcaggcacc
      481 cgcagttatc ttacccttcc tcaacaaaat agctgtttat ttatacagca aatcctgttt
      541 cggacttctc actccccatt tgatcaactg aagctgaacc cgtttttttc tgcatgcctc
      601 aatcgtcaaa attcttcgcc accagccagc tctgccataa cttgccacat tgaaaaagta
      661 gccaaagtgc ttcaggcacc atcagttcac catcaccaaa aaaaagaaac attccagaac
      721 ttccgcatct cgcccagccg ctccgacttc tatactgaat agaaaacgtc aacttaagag
      781 aaacctatgc cattacccga ttttcatttt tctgaacctt ttaccctcgg tattgaactg
      841 gaaatgcagg tggttaatcc gccgggctat gacttaagcc aggactcttc aatgctgatt
      901 gacgcggtta aaaataagat cacggccgga gaggtaaagc acgatatcac cgaaagtatg
      961 ctggagctgg cgacggatgt ttgccgtgat atcaaccagg ctgccgggca gttttcagcg
     1021 atgcagaaag tcgtattgca ggcagccgca gaccatcatc tggaaatttg cggcggtggc
     1081 acgcccccgt ttcagaaatg gcagcgtcag gaggtatgcg acaacgaacg ctatcaacga
     1141 acgctggaaa actttggcta tctcatccag caggcgaccg tttttggtca gcatgtccat
     1201 gttggctgtg ccagtggcga tgacgccatt tatttgctgc acggcttgtc acggtttgtg
     1261 ccgcatttta ttgccctttc cgccgcgtcg ccatatatgc agggaacgga tacgcgtttt
     1321 gccccctcac gaccgaatat tttttccgcc tttcctgata atggcccgat gccgtgggtc
     1381 agtaactggc aacaatttga agccctgttt cgctgtctga gttacaccac gatgatcgac
     1441 agcattaaag atctgcactg ggatattcgc cccagtcctc attttggcac ggtggaggtt
     1501 cgggtgatgg ataccccgtt aacccttagc cacgcggtga atatggcggg attaattcag
     1561 gccaccgccc actggttact gacggaacgc ccgttcaaac atcaggagaa agattacctg
     1621 ctgtataaat tcaaccgttt ccaggcctgt cgctatggtc tggaaggcgt cattaccgat
     1681 ccgcacactg gcgatcgtcg accgctaacg gaagatacct tgcgattgct ggaaaaaatc
     1741 gccccttcag cacataaaat tggcgcatcg agcgcaattg aagcactgca tcgccaggtc
     1801 gtcagcggtc tgaatgaagc gcagctgatg cgcgatttcg tcgccgatgg cggctcgctg
     1861 attgggctgg tgaaaaagca ttgtgagatc tgggccggtg actaagccgg gaattgcctt
     1921 acagaagaat atccctgaca atggtgtttt aacttacatt taacaatgtt atgaaacacc
     1981 ctttagaaac cttgaccacc gcagcaggca ttttgctgat ggctttcctc tcttgcctgc
     2041 tgctgcccgc ccccgcactg gggctgacgc tggcacaaaa actggtgacc atgttccatc
     2101 tgatggatct tagccagctt tacactttat tgttttgtct gtggttttta gtgctgggcg
     2161 ctattgagta ttttgttctg cgctttatct ggcgacgctg gttctcgctg gcggattaaa
     2221 cgtcgattat cgccgaactt cgcggcatac tttgcttatt ctccttcgcc taaaggaacg
     2281 tttatggata agcaatcact gcacgaaacg gcgaaacgcc tagctcttga gttacccttt
     2341 gtcgagcttt gctggccttt tggcccggag ttcgatgttt ttaaaattgg cggcaagatt
     2401 tttatgctgt cgtcggagct acgcggcgtc ccctttatca atctgaagtc cgatccacaa
     2461 aaatccctgt taaatcagca aatataccca agcattaagc cagggtatca catgaataaa
     2521 aagcactgga tttcggtgta ttccggcgag gaaatctccg aagcgttact tcgcgatctg
     2581 atcaacgatt cgtggaatct ggtggttgat ggtctggcta aacgcgatca aaaaagagtg
     2641 cgtccaggct aaagcgggaa tctatagcgc atttttctcg cttaccattt ctcgttgaac
     2701 cttgtaatct gctggcacgc aaaattactt acacatggag tctttatgaa tatcatttct
     2761 gtcgccttaa agcgtcattc cactaaggca tttgatgcca gcaaaaaact taccccggaa
     2821 caggccgagc agatcaaaac gctactgcaa tacagcccat ccagcaccaa ctcccagccg
     2881 tggcatttta ttgttgccag cacggaagaa ggtaaagcgc gtgttgccaa atccgctgcc
     2941 ggtaattatg tgttcaacaa acgtaaaata cttgatgcct cgcacgtcgt ggtgttctgt
     3001 gcaaaaaccg cgatggacga tgcctggctg aagctggttg ttgaccagga agatgccgat
     3061 ggccgctttg ccacgccgga agcgaaagcc gcgaacgata aaggtcgcaa gttcttcgcc
     3121 gatatgcacc gtaaagatct gcatgatgat gcagagtgga tggcaaaaca ggtttatctc
     3181 aacgtcggta acttcctgct cggcgtggcg gcgctgggtc tggacgcggt gcccatcgaa
     3241 ggttttgacg ccgcaatcct cgatgcagaa tttggtctga aagagaaagg ctacaccagt
     3301 ctggtggttg ttccggtagg tcatcacagc gttgaagatt ttaacgctac gctgccgaaa
     3361 tctcgtctgc cgcaaaacac caccttaacc gaagtgtaat tctctcttgc cgggcatctg
     3421 cccggctatt tcccctcaga ttctcctgat ttgcataacc ctgtttcagc cgtcatcata
     3481 ggctgctgtt gtataaagga gacgttatgc aggatttaat atcccaggtt gaagatttag
     3541 cgggtattga gatcgatcac accacctcga tggtgatgat tttcggtatt atttttctga
     3601 ccgccgtcgt ggtgcatatt attttgcatt gggtggtact gcggaccttc gaaaaacgtg
     3661 ccatcgccag ttcacggctt tggttgcaaa tcattaccca gaataaactc ttccaccgtt
     3721 tagcttttac cctgcagggg attatcgtca atattcaggc ggtattctgg ctgcaaaaag
     3781 gcaccaaagc ggcagatatt ctgactacct gcgcgcagtt gtggatcatg atgtatgcgc
     3841 tgctttcagt cttctcgttg ctggatgtta ttttgaatct ggcgcagaaa ttcccggcgg
     3901 catctcagtt accgctgaaa gggatatttc aggggattaa actgatcggc gcaatcctgg
     3961 tcggcatttt gatgatctcg ctgctgattg gtcagtcgcc agcgattctg atcagcggtc
     4021 ttggtgcaat ggctgccgtg ctgatgttgg tatttaaaga tccgattctt ggtctggtgg
     4081 caggtattca gctttccgcg aacgatatgc tgaaactggg cgactggctg gaaatgccga
     4141 aatacggcgc ggatggcgcg gtgatcgata ttgggttaac caccgtcaaa gtgcgtaact
     4201 gggacaatac cattaccact attcccacct ggtctctggt ttctgactcc tttaaaaact
     4261 ggagcgggat gtcagcatct ggcgggcgac gtattaagcg cagtatcagt attgatgtca
     4321 ccagtattcg ttttcttgat gaagacgaaa tgcaacgtct gaataaagcg catttgttaa
     4381 agccttattt aaccagccgc catcaggaaa ttaatgagtg gaatcgccag caaggttcta
     4441 cggagtcggt attaaacctg cgccgaatga ccaatattgg aacctttcgt gcctatctga
     4501 acgaatatct gcgtaaccat ccgcggattc gtaaagatat gaccttaatg gtacgccaac
     4561 tggctccagg tgataacggt ttaccgctcg agatctacgc gtttactaac accgtggtgt
     4621 ggctggaata tgaaagcatt caggctgata tattcgatca catatttgcg attgtcgaag
     4681 agtttggtct gcgacttcat cagtcgccaa ccggcaatga tattcgctct ctggcgggtg
     4741 catttaagca gtaattaaaa aaaccgctct catcgaatgg atgagagcgg acatcgtttt
     4801 gtcggatgta gcgtgaatgc cttatttccg acgcagcgtt ttaaatgcca caaacaggaa
     4861 tacaatccac accggcagca ggatcgctga caagcgcata tcatccatcg tgcacatcag
     4921 cagcaaaatc atgccgagga aggcgatgca aagatagttg ccgaacggat agagcagcgc
     4981 cttaaactgt gtttcacgcc cctgacgtcg catcgccgcg cgaaaacgca gatgcgccag
     5041 gcagatcata atccagttca acagcaacgt tgctaccacc agcgtcatca gcagaccaaa
     5101 cgctttttgc ggcagcagat agttgattaa caccaccagc gaagtgatcg ctccggaaag
     5161 catcagcgag ttaatcggca caccgcgacg gctgacgcga gtcaaaaact tcggcgcatt
     5221 accctgcaca gaaaggccaa acagcatgcg gctgttagag taaaccccgc tgttatacac
     5281 cgacagcgat gccaccagaa tgacgaagtt cagcgcagaa gctaccacat tgctgtcgag
     5341 attatggaaa atcatcacaa acgggctact gttggatttc acttccaccc acggatagag
     5401 tgccagtaaa accaccagtg aaccgatata aaacagcagg attcgataca ccacctgatt
     5461 taccgctttt ggaatgcttt tttccggatc gcgcgcttca gcggctgtaa tcccaatcag
     5521 ctccagaccg ccgaaggaga acataattac cgccagcgac aaaatcagcc cattccggcc
     5581 ggtggcgaag aaaccaccgt agcgccagag gttgtcgata ctggctttct cgccgccgtg
     5641 accagaaaac agcagccaca ggccaaagcc gatcataccg atgatcgcca gcactttaat
     5701 cagcgcaaac cagaactcgg tttcgccata taagcgcacg ttcaccaggt taacggcgtt
     5761 gatgataata aagaaggcgg cagcccaaat ccacgttgga acatccggga accagtactg
     5821 catatagatg cccgcagcgg tcagctctgc cattcccacc agcacgaaca ttacccagta
     5881 gttccagcca gagaggaagc ccgcaaacgg tccccagtat ttataggcaa agtgggcaaa
     5941 tgaaccggat accggctctt cgaccaccat ctcgccaagc tggcgcataa tcaggaaagc
     6001 gatgatcccg gcgacgccgt agcccagcaa tacagccgga cccgccatct gaatcgccgg
     6061 gccaatgcca agaaacagac cagtaccaat tgcgtcaccc agcgcaatca gttgaatatg
     6121 acggttatgt aatccgcgat gaagcgtcgg ctcttgattc gacgcagtat cttccgatac
     6181 ggttgacgcg tttttcacgc ctttcccctg tgtgtctttt ttgttgaggg gcacctttta
     6241 acatttagtg cccatcgtcg caagacacaa tccacacggt taaacgaggt atcctgcttt
     6301 aattccgtac ccgatgtcgg tgcagccaca tcagcttata cgccgccggg ataataaaca
     6361 gcgacagcaa aggtgcggtg atcatgccgc caatcatcgg tgcggcaatc cggctcatca
     6421 cctctgaacc agcccccgtt ccccacagaa tcggcagcag accggcgata atcaccgcca
     6481 ccgtcatcgc tttcgggcgc acgcgcagga ccgcgccgtg atacaacgcc tcatccagct
     6541 tctgctcgct gaatgtttgt ggattattca acgacggctc ggcctctatg gcgtgacgta
     6601 aatacatcag catcaccacg ccaaattcgg cggcgacccc ggcaagggca ataaagccgg
     6661 tacccgtcgc cacagaaaga tgaaagccca tccaccacag taaccagatg ccgcccacca
     6721 gcgcaaacgg tacgctgctg ataatcagca acgcttcgcc cacccgacgg aatgccagat
     6781 acaacagcac gaagatgatc atcagtgtca tcggcaccat gagtttcagc ttatggttgg
     6841 cgcgctccag tagctcgaac tgcccggaga atgccacgct ggtgcccggt tttaactgca
     6901 ctttttcagc tatcgctttt tgcaaatcgt gaaccaccga caccatgtca cgatcgcggg
     6961 catcgatata aatccagctc gtcgggcgcg cattctcggt tttcagcatc gacggtccgg
     7021 tagagacttt aacgtcggcc acgtctgcca gggtgatttg ctgcttcatc ggcgtcagga
     7081 tcggcaactg gcgcagtgcc tgcggactat cgcgccagct ttgcggataa cgcagattaa
     7141 ttggataacg ggcaatccct tccaccgttt cgccaaccat cgccccgccc accgcagaag
     7201 tcacaaacaa ctgcacatcc gccaccgtca taccgtaacg tgcggctttt tcacggttaa
     7261 tctcaacgtt gatatagcgc ccaccttcca gccgctcggc aagcgcagaa gctacgcctg
     7321 gcaccgttcg cgccacttct tcaatttgct cagccatcgc gtcgatatcc gccagcacag
     7381 tgccggaaac tttaatgccg atggggcttt taatgccggt tgagagcata tcgatacggt
     7441 tacgaattgg cggcacccac agattcgcca gccccggcag acgcacggtg ttatccagtt
     7501 cctcaatgat tttgtccatt gtcatgcctg gccgccactg atcctgtggc ttaagctgga
     7561 tggtcgtttc taccatctcc agcggagcag aatcggtggc ggtttccgct ttcccggttt
     7621 tgccaaatac ccgcgccact tcaggtacgc tcataattag cttgtcggtt ttttgcagca
     7681 tactcgccgc ctctgctgcg gaaatccccg gcagcgtcga tggcatatac aacaagtcgc
     7741 cttcattgat ctgcggtaaa aattccccgc caactttatt gagcggccag agaaccgtca
     7801 gcaccgaaag cgccgccacc agcagcgtgg ttttcggcca gtgcagtact ttcagcaaca
     7861 gcggatgata aacacgaatc aaaaagcgat tgagcggatt actgctttcc ggcggaattt
     7921 tgccacggat ccagtaaccc atcaggatag ggatcactac gatcgccagc agcgccgcac
     7981 ccgccatcgc atacgttttg gtgaacgcca acgggccaaa cagacgccct tcctgccctt
     8041 ccagggtgaa gatcgggata aacgacaacg tgataatcag cagactgata aacagcgccg
     8101 gccccacttc aacagacgca tcggtgatca cctgccagcg cgttttatta tccagcgtgg
     8161 cgtcaggatg ctggtgctgc cactcttcca gccgtttatg cgcattctcg atcatgacga
     8221 tagcggcatc gaccatcgcc ccgacggcaa tcgcaatgcc acccagcgac ataatattgg
     8281 cattcagccc ctggaagggc atgacaataa aagcaataca caaccccagc ggcaacgaaa
     8341 taatcgccac cagcgccgag cgcacatgcc agagaaacag cgcgcagact accgccacca
     8401 caataaactc ttccagcaac ttgccgctga ggttgtcgat ggcccggtca atgagctggc
     8461 tgcggtcgta tgttgtaact atctccacgc cttccggcag actacttttc agcgtttcca
     8521 gtttgtcctt cacggcggcg atcacttctc gggcgttttt gccggatcgc aggatcacca
     8581 ccccgcccgc cacttcgcct tcgccgttta atgccccggc gcatctccgg gccaatctgg
     8641 tccttcgcaa catcgcgcag ataaacgggc acgccatttt cactggcttt taaaacgatg
     8701 tgattaaagt cgtcgagcgt ttgtagatag ccgctggcgc gcaccatata ttctgcttcc
     8761 gccagttcga ttgacgaacc gcccgcttcc tggtttgaag catccagcgc gctttttact
     8821 tcggcgagac tgatgccata ctgcgccagg cgctgaggat cgataaccac ctgatactct
     8881 ttcaccacac cgcctaccga cgccacttcc gcaacgtcag gaatggtttt cagctcatat
     8941 ttgagaaacc agtcctgcaa tgagcgtaaa tcggcaagat cgtgcttacc gctgcgatcc
     9001 accagtgcat attcatagat ccagccaaca cccgtggcat ctggtcccag ctcggcgctg
     9061 actcccgcag gcagcttacc ctgtacctgg ttgaggtact ccagcacccg cgaacgcgcc
     9121 cagtacggat cggtgccatc ttcgaaaatg acatacacat aagagtcgcc aaactgcgag
     9181 aaaccacgca cagtcttcgc gccaggcacc gacaacatgg tggtggttag cggataagtc
     9241 acctgatttt caacgatttg cggtgcctgg ccgggatagc tggttttaat aatcacctgc
     9301 acatcggaga gatccggcaa cgcatccact ggcgtattaa tgatggtcca ggtgccccag
     9361 atgctcagaa acaacgcgcc catcagcacc aaaaaacggt tcgccaccga gcgacgaata
     9421 atccattcaa tcattggtta ttccctcaat gcgcatgggt agcactttca gagcgcatcc
     9481 gctccagtgc gccagaaata ttggcttcgg aatcaatcag gaacaggccg ccggaaacca
     9541 ccttttcacc ttccgccaga ccagagcgta atgcggtgac gccttgcgat gcctggaaaa
     9601 cagcaacgcg tttcggtaca aagcgcccgt cggcatcaac ggtaatcacc cgctgttcgt
     9661 tgccggtatc aatcagcgcc tcgtcggcgt tgtcgacttc cagacgcagc tgcagcgtgc
     9721 gggtcgcggc atccacgcca ggtagcagcg tccatttgcg gatggtgagt gttttatccg
     9781 gtcgcgccgg aacggtgagg gtaaactgcg aggcatcttt caccagccag gcgatggact
     9841 ccgggatcgc agcagtgacc cacaccgggt ccataccctg aattcgcgat tacgcgcaat
     9901 acccctgaga accgccatga acaagggttt catgatttgt gattttagtt tagtacgcaa
     9961 tttggtacac aacactattt tcacttcagc gggtagtcat caaactcacc ataacgagca
    10021 tcgttgatga tgtatgtgat caccacaaat atcacatctg gactagttcc gtcttcatgc
    10081 gcag
//
LOCUS       DAETPZ010000123         9958 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.124, whole genome shotgun sequence.
ACCESSION   DAETPZ010000123 DAETPZ010000000
VERSION     DAETPZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9958
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.124"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="K0W59_003593"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="K0W59_003593"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBI3024471.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="K0W59_003594"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="K0W59_003594"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HBI3024472.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3326)
                     /locus_tag="K0W59_003595"
                     /pseudo
     CDS             complement(2698..3326)
                     /locus_tag="K0W59_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3456..5216
                     /locus_tag="K0W59_003596"
     CDS             3456..5216
                     /locus_tag="K0W59_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HBI3024473.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5622..6479
                     /gene="focA"
                     /locus_tag="K0W59_003597"
     CDS             5622..6479
                     /gene="focA"
                     /locus_tag="K0W59_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HBI3024474.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6534..8816
                     /gene="pflB"
                     /locus_tag="K0W59_003598"
     CDS             6534..8816
                     /gene="pflB"
                     /locus_tag="K0W59_003598"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBI3024475.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRISTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9008..9748
                     /gene="pflA"
                     /locus_tag="K0W59_003599"
     CDS             9008..9748
                     /gene="pflA"
                     /locus_tag="K0W59_003599"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HBI3024476.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         2553 a         2495 c         2377 g         2533 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcttggc taatccgcgc cagctgctcg aaataatccg gaaatgtttt ggccgtgcat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg aggagtgata
      241 cgaatgtaat cgtgcccctc ttccacttcc gcgccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcaggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc caggaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gctctttcat acgcggctca ccggtcagca caatatcatt gctatccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttaaccc taacgctgtt aatgcattca gcatatggcg cacgtcgtcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgcaag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggca ttataaatag acgcgcgcat
     1501 tccgccgacc acacggtgtc ctttcagtgc atgaaggcct gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaattaca ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtctcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc cgcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgcgttca tatgaatgat ttcactacaa cgaatgcagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccatta aagaactcaa
     3001 attccgtcat gttggagtta ttgagtttcg accaggccag ctcgccatca cattaccaag
     3061 cggtataaca acgccagagt gataaagacg ataagtaccg gcaccatcgg cacatcatca
     3121 taaaacgcca catctcccgc cgctgaaccc agcgtgagaa tgattaacac ttcaaacagc
     3181 gacatctgtc gcacaccgcg tcttccggtc attttgagga acaaaaaaac cagtacaaag
     3241 gtataaagac tacgtagtgc aacttcgcca aggaaatcaa aaggcacttt atcaaatgcc
     3301 atacggtgga gatcgaacgc cttcattttt atgctcttaa cgtggtttat ccgctaaaag
     3361 catagtcaac agttcatcgg catggcggcg gtgatagcgc ctttgagata aaacccgtat
     3421 tattgcgcgc tttccgtacg actaaagtga ttttcatgac gcaaacattt atccccggca
     3481 aagatgccgc tctggaagat tccatcgctc gcttccagca aaaactttca gacctcggct
     3541 ttcagattga agaggcctcc tggctgaatc ccgtgcctaa cgtctggtct gtacatattc
     3601 gcgacaaaga gtgcgcactg tgttttacca acggtaaagg cgcaaccaag aaagcggcgc
     3661 tggcttctgc actcggtgaa tatttcgagc gtctctcaac caactacttt tttgctgatt
     3721 tctggctggg cgaaaccatc gccaacggtc catttgtgca ttatcccaac gaaaaatggt
     3781 tcccactgag cgaaaatgac gatgtgccag aagggctgct cgatgaccgt ctgcgcgcat
     3841 tttacgatcc ggagaatgaa ttgaccggca gtatgctgat tgacctacaa tccggtaacg
     3901 aagatcgtgg tatttgcggt ctgccgttta cgcgccagtc cgacaatcag accgtttata
     3961 ttccgatgaa tatcattggt aacctgtacg tttctaacgg tatgtccgct ggcaataccc
     4021 gcaacgaagc acgcgttcag gggttgtccg aagttttcga acgctacgtg aaaaaccgca
     4081 ttattgctga aagcatcagc ttgccggaaa tcccggcaga cgtgctggcg cgttacccag
     4141 cggtggttga agcgatcgaa acactggaaa cagaaggttt cccgatcttc gcatatgatg
     4201 gttcccttgg cggccagtat ccggtgattt gcgtggtact gtttaatcct gcaaacggca
     4261 cctgctttgc ctctttcggt gcgcatcctg attttggcgt agcactggaa cgtaccgtga
     4321 ccgagctgct gcaaggtcgt ggcctgaaag atttggatgt gtttactccg ccaaccttcg
     4381 atgatgaaga agtcgctgaa cataccaacc tcgaaacgca ctttatcgat tccagcggtt
     4441 taatctcctg ggacctgttc aagcaggatg ccgattatcc gtttgtggac tggaatttct
     4501 ccggcaccac ggaagaagag tttgctacgc tgatggctat cttcaacaaa gaagataaag
     4561 aagtttatat tgccgattac gagcatctgg gcgtttatgc ttgccgtatt atcgtgcctg
     4621 gcatgtccga tatttatccg gctgaagatc tgtggctcgc gaataacagt atgggcagcc
     4681 atttacgtga aacgattctt tcgctaccag gcagcgagtg ggaaaaagaa gattacctga
     4741 acctcatcga gcaactggat gaagaaggtt ttgatgactt tacccgcgtg cgtgagctgt
     4801 tgggtctggc gaccgggtcg gataacggtt ggtacaccct gcgtatcggt gaattaaaag
     4861 ccatgctggc gctggctggt ggcgatctgg aacaggctct ggtctggacc gaatggacga
     4921 tggagtttaa ctcatcagta tttagtccgg aacgcgccaa ctattatcgc tgcctgcaaa
     4981 cgttgttatt actggcacag gaagaagatc gccagccgct gcaatatctg aatgcgtttg
     5041 ttcgcatgta cggcgcagat gccgtagaag ccgccagtgc ggcaatgagc ggcgaagcgg
     5101 cgttttacgg cctgcaaccg gtagatagcg atctgcacgc gtttgctgca catcagtcgt
     5161 tgttgaaggc ctacgaaaag ctgcagcgcg ccaaagcagc attctgggca aaataaaatc
     5221 aaatagccta cgcaatgtag gcttaatgat tagtctgagt tatattacgg ggcgtttttt
     5281 taatgccccg ctttacatat atttgcatta ataaaataat tgtaattata aggttaaata
     5341 tcggtaattt gtatttaata aatacgatcg atattgttac tttattcgcc tgatgctccc
     5401 ttttaattaa ctgttttagc ggaggatgcg gaaaaaattc aactcatttg ttaattttta
     5461 aaatttattt ttatttgggt aatcaaatag ttactccgta tttgcataaa aaccatgcga
     5521 gttacgggcc tataagccag gcgagatatg atctatatca atttctcatc tataatgctt
     5581 tgttagtatc tcgtcgccga cttaataaag agagagttag tgtgaaagct gacaaccctt
     5641 ttgatctttt acttcctgct gcaatggcca aagtggccga agaggcgggt gtctataaag
     5701 caacgaaaca tccgcttaag actttctatc tggcgattac cgccggtgtt ttcatctcaa
     5761 tcgcattcgt cttctatatc acagcaacca ctggcacagg cacaatgccc ttcggcatgg
     5821 caaaactggt tggcggcatt tgcttctctc tggggctgat tctttgtgtt gtctgcggag
     5881 ccgatctctt tacttccaca gtgttgattg ttgttgctaa ggcgagtggg cgcatcacct
     5941 ggggtcagtt ggctaaaaac tggctaaatg tctattttgg caacctggtc ggcgcactgc
     6001 tgtttgtact tttaatgtgg ctttccggcg agtatatgac cgcaaatggt caatggggac
     6061 taaacgtcct acaaaccgcc gaccacaaag tgcaccatac ttttattgag gccgtctgtc
     6121 ttggtatcct ggcaaacctg atggtatgtc tggcagtatg gatgagttat tctggccgca
     6181 gcctgatgga caaagcgttc attatggtgc tgccggtcgc gatgtttgtt gccagcggtt
     6241 ttgagcacag tatcgcaaac atgtttatga tcccgatggg tattgtaatc cgcgacttcg
     6301 catctccgga attctggacc gctgtcggtt ctgcaccgga aaatttttct cacctgaccg
     6361 tgatgaactt catcactgat aacctgatcc cggttacgat cggtaatatt atcggcggtg
     6421 gtttgttggt tgggttgaca tactgggtca tttacctgcg tgaaaacgat caccattaat
     6481 ggttgtcgaa gtacgcagta aataaaaaat ccacttaaga aggtaggtgt tacatgtccg
     6541 agcttaatga aaagttagcc acagcctggg aaggttttac caaaggtgac tggcagaatg
     6601 aagtaaacgt ccgtgacttc attcagaaaa actacactcc gtacgagggt gacgagtcct
     6661 tcctggctgg cgctactgaa gcgaccacca ccctgtggga caaagtaatg gaaggcgtta
     6721 aactggaaaa ccgcactcac gcgccagttg attttgacac cgctgttgct tccaccatca
     6781 cctctcacga cgctggctac atcaataaag cgttggaaaa agttgttggt ctgcagactg
     6841 aagctccgct gaaacgtgct cttatcccgt tcggtggtat caaaatgatc gaaggttcct
     6901 gcaaagcgta caaccgcgaa ctggacccga tgatcaaaaa aatcttcact gaataccgta
     6961 aaactcacaa ccagggcgtg ttcgacgttt acactccgga catcctgcgt tgccgtaaat
     7021 ccggtgttct gaccggtctg ccagatgctt atggccgtgg ccgtatcatc ggtgactacc
     7081 gtcgcgttgc gctgtacggt atcgactacc tgatgaaaga caaatacgct cagttcacct
     7141 ctctgcaggc tgatctggaa aacggcgtaa acctggaaca gactatccgt ctgcgcgaag
     7201 aaatcgctga acagcaccgc gctctgggtc agatgaaaga aatggctgcg aaatacggct
     7261 acgacatctc tggtccggct accaacgctc aggaagctat ccagtggact tacttcggct
     7321 acctggctgc tgttaagtct cagaacggtg ctgcaatgtc cttcggtcgt atctccacct
     7381 tcctggatgt gtacatcgaa cgtgacctga aagctggcaa gatcaccgaa caagaagcgc
     7441 aggaaatggt tgaccacctg gtcatgaaac tgcgtatggt tcgcttcctg cgtactccgg
     7501 aatacgatga actgttctct ggcgacccaa tctgggcaac cgaatctatc ggtggtatgg
     7561 gcctcgatgg tcgtaccctg gttaccaaaa acagcttccg tttcctgaac accctgtaca
     7621 ctatgggtcc gtctccggaa ccgaacatga ccattctgtg gtctgaaaaa ctgccgctga
     7681 acttcaagaa attcgccgct aaagtgtcca tcgacacctc ttctctgcag tatgagaacg
     7741 atgacctgat gcgtccggac ttcaacaacg atgactacgc tatcgcttgc tgcgtaagcc
     7801 cgatgatcgt tggtaaacaa atgcagttct tcggtgcgcg tgcaaacctg gcgaaaacca
     7861 tgctgtacgc aatcaacggc ggcgttgacg aaaaactgaa aatgcaggtt ggtccgaagt
     7921 ctgaaccgat caaaggcgat ctcctgagct acgatgaagt gatggagcgc atggatcact
     7981 tcatggactg gctggctaaa cagtacatca ctgcactgaa catcatccac tacatgcacg
     8041 acaagtacag ctacgaagcc tctctgatgg cgctgcacga ccgtgacgtt atccgcacca
     8101 tggcgtgtgg tatcgctggt ctgtccgttg ctgctgactc cctgtctgca atcaaatatg
     8161 cgaaagttaa accgattcgt gacgaagacg gtctggctat cgacttcgaa atcgaaggcg
     8221 aatacccgca gtttggtaac aatgatccgc gtgtagatga cctggctgtt gacctggtag
     8281 aacgtttcat gaagaaaatt cagaaactgc acacctaccg tgacgctatc ccgactcagt
     8341 ctgttctgac catcacttct aacgttgtgt atggtaagaa aactggtaac accccagacg
     8401 gtcgtcgtgc tggcgcgccg ttcggaccgg gtgctaaccc gatgcacggt cgtgaccaga
     8461 aaggtgctgt agcgtctctg acttccgttg ctaaactgcc gtttgcttac gctaaagatg
     8521 gtatctccta caccttctct atcgttccga acgcactggg taaagacgac gaagttcgta
     8581 agaccaacct ggctggtctg atggatggtt acttccacca cgaagcatcc atcgaaggtg
     8641 gtcagcacct gaacgttaac gtgatgaacc gtgaaatgct gctcgacgcg atggaaaacc
     8701 cggaaaaata tccgcagctg accatccgtg tatctggcta cgcagtacgt ttcaactcgc
     8761 tgactaaaga acagcagcag gacgttatta ctcgtacctt cactcaatct atgtaattag
     8821 atttgactga aatcgtacag taaaaagcgt acaataaagg ctccacgaaa gtggggcctt
     8881 ttttagcacg agagcctttt ttgtcagcta tctatacttt aaggtgactg ccaaaacaga
     8941 ctcgacgtag ccttcgagct gcgcaccaac acggcctcag atgggccaca tctggagaaa
     9001 caccgcaatg tcagttattg gtcgcattca ctcctttgaa tcctgtggaa ccgtagacgg
     9061 cccaggtatt cgctttatca cctttttcca gggctgcctg atgcgctgcc tgtattgtca
     9121 taaccgcgac acctgggaca cgcatggcgg taaagaagtt accgttgaag atttgatgaa
     9181 ggaagtggtg acctatcgcc actttatgaa cgcttccggc ggcggcgtta ccgcatccgg
     9241 cggtgaagca atcctgcaag ctgagtttgt tcgtgactgg ttccgcgcct gtaaaaaaga
     9301 aggcattcat acctgtctgg ataccaacgg ttttgttcgt cgttacgatc cggtgattga
     9361 tgaactgctg gaagtaaccg acctggtaat gctcgatctc aaacagatga acgacgagat
     9421 ccaccaaaat ctggttggag tttccaacca ccgcacgctg gagttcgcta aatatctggc
     9481 gaacaaaaat gtgaaggtgt ggatccgcta cgttgttgtc ccaggctggt ctgacgatga
     9541 cgattcggcg catcgcctcg gtgaatttac ccgtgatatg ggtaacgttg agaaaatcga
     9601 acttctcccc taccacgaac tgggcaaaca caaatgggtg gcgatgggtg aagaatacaa
     9661 actcgatggt gttaaaccac cgaagaaaga gaccatggaa cgcgtgaaag gcattcttga
     9721 gcagtacggt cataaggtaa tgttctaatt tccccgtaaa gcggcaactt tattgagttg
     9781 ccgctttttt attccgcgct tcattctgct acagcgtttt taacacatct tatagtgcat
     9841 ctaccatatc ccgtacctga gataataacg actgataata gctcgcgtga agttcttccc
     9901 catcgaatac cactgtatta cacaagaaag tgacatcggc atcttcaata ccaagata
//
LOCUS       DAETPZ010000124         9527 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.125, whole genome shotgun sequence.
ACCESSION   DAETPZ010000124 DAETPZ010000000
VERSION     DAETPZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.125"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(437..643)
                     /locus_tag="K0W59_003600"
     CDS             complement(437..643)
                     /locus_tag="K0W59_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HBI3024477.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMFFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            956..1045
                     /gene="kdpF"
                     /locus_tag="K0W59_003601"
     CDS             956..1045
                     /gene="kdpF"
                     /locus_tag="K0W59_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBI3024478.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1045..2718
                     /gene="kdpA"
                     /locus_tag="K0W59_003602"
     CDS             1045..2718
                     /gene="kdpA"
                     /locus_tag="K0W59_003602"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBI3024479.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAILNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2741..4788
                     /gene="kdpB"
                     /locus_tag="K0W59_003603"
                     /pseudo
     CDS             2741..4788
                     /gene="kdpB"
                     /locus_tag="K0W59_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4797..5369
                     /gene="kdpC"
                     /locus_tag="K0W59_003604"
     CDS             4797..5369
                     /gene="kdpC"
                     /locus_tag="K0W59_003604"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBI3024480.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5362..6345
                     /locus_tag="K0W59_003605"
                     /pseudo
     CDS             5362..6345
                     /locus_tag="K0W59_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381859.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
     gene            6345..8044
                     /locus_tag="K0W59_003606"
                     /pseudo
     CDS             6345..8044
                     /locus_tag="K0W59_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141249.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
     gene            8041..8718
                     /gene="kdpE"
                     /locus_tag="K0W59_003607"
     CDS             8041..8718
                     /gene="kdpE"
                     /locus_tag="K0W59_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HBI3024481.1"
                     /translation="MTNVLIVEDEQAIRSFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8993..9097
                     /locus_tag="K0W59_003608"
     CDS             8993..9097
                     /locus_tag="K0W59_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HBI3024482.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9408..>9527
                     /locus_tag="K0W59_003609"
     CDS             9408..>9527
                     /locus_tag="K0W59_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBI3024483.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSV"
BASE COUNT         2118 a         2396 c         2640 g         2373 t
ORIGIN      
        1 aacataacat cttattggat tagatatgtt aacaattaat tattcttaat atatcaataa
       61 agtatatatt actctcttta ctatctgttt ttattgcggt agcgatacta ataacaaaat
      121 tcggaattta tttagatgct ggaattagaa aaagggatga cggctttctc accttaccct
      181 gaagtttcac gaaagtgtgt aaggaagtat gcaatctaac tggagatttc tgatgatcta
      241 gtcgcttaga gcgacaatga aggtgaccgc atcctgggta gccctatgaa aggatactat
      301 tgaaacgtga tgctaaaaca gtgatgaaat gaataatacg atggaaacgt cttccatgtt
      361 aattatcatg gaagacgttg atgttgaaca cctaaaccag ccccctgata cgaggctggc
      421 ttaagcgcgc aggcaatcag taaaaatcac cggttgcctt ttcggcctga tccatccaca
      481 ccggtttatc gctggttttc gaccagacgc ggtgcaggta gctgtaaaag cgggcgcggt
      541 ctttccagaa aaacatgaaa ggcagcgcca gaacgcccgc tgcaaccgca tatgtacggc
      601 gtaaaaagat aagccatgca ggatattctc tgtagagttc catacttttc tccccctttt
      661 gtatctcccc ggtgaatagc accggaaaaa tgaatttgtt tatctgatga aaatagtacc
      721 gccttttgtg taattttacc actcatccga ccacttattt ttgcttattg atggtttatt
      781 tacattcaca ctgtaattaa attacacaaa agttaaatta atactaacca tcagttaaat
      841 catggccttt gccattttta tacttttttt acaccccgtc cgcagatttt tgcgaaatct
      901 ttgcagccag aattctactc ttccggtatc acttttaggc tactggaggt gcactgtgag
      961 tgcaggcgtg atagccggcg tgttgctggt gtttttatta ctgggttatt tggtttatgc
     1021 cctgatcaat gcggaggcgt tctgatggct gcgcaagggt tcttactgat tgccacgttt
     1081 ttactggtat taatggtgct ggcgcgtcct ttaggcagcg ggctggcgcg gctgattaat
     1141 gacattcctc ttccaggtac agcgggcgtt gaacgtatac tctttcgttt acctggcgtc
     1201 tctgaccatg agatgaactg gaagcaatat ctttgtgcca ttctcggcct gaacatgctg
     1261 gggctggcgg tgctgttttt tatgttgctc ggtcagcact atctgccgct taatccacag
     1321 cagttgccgg ggctgtcgtg ggatctggcg ctgaataccg ccgtcagctt tgtcacgaat
     1381 accaactggc aatcttatag cggtgaaacc acgttgagct atttcagcca gatggcaggc
     1441 ttaacggtac aaaactttct ttctgccgcc agcgggattg cggtgatttt tgccttcatc
     1501 cgtgcgttta ctcgccagag catgagcaca ctcgggaatg cctgggtcga tctgctacgc
     1561 atcacgttat gggtgctagt ccctgtggcg ttgttaatcg cactgttttt tattcaacaa
     1621 ggtgcgctgc aaaactttct gccctatcag gcagtgaata ccgttgaagg agcgcaacag
     1681 ctgttaccca tgggaccagt agcttctcag gaagcgatca agatgctcgg tactaacggc
     1741 ggtggcttct ttaatgccaa ctcgtcgcat ccgtttgaaa acccaaccgc actgaccaac
     1801 ttcgtgcaga tgctggcgat cttcttgatc ccaacagcgc tgtgctttgc ctttggtgaa
     1861 gtgacgggcg atcgccgcca ggggcgcatg ttgctgtggg cgatgtcagt gatttttgtc
     1921 atctgcgtag gcgtggttat gtgggcagaa gttcagggta atcctcatct gctggcgctc
     1981 ggcgcagaca gcagcatcaa tatggaaggt aaagagagcc gtttcggcgt gctggtcagt
     2041 agcctgtttg cggtcgtgac tacggcggcg tcctgtggcg cggtgattgc gatgcatgat
     2101 tcgtttaccg ctctcggcgg catggtgccg atgtggctaa tgcaaattgg tgaagtggtg
     2161 ttcggcggtg tcggttctgg tctttacggc atgatgctgt ttgtcctgct ggcggtgttt
     2221 attgccgggc tgatgattgg tcgtacaccg gaatatctgg gtaaaaaaat cgacgtacgc
     2281 gagatgaaac tgactgcact ggcgattctg gttactccaa cactggtact gatgggcgcg
     2341 gcgttggcga tgatgaccga cgccggacgt agcgccatac ttaaccctag cccgcatggt
     2401 tttagcgaag tgctgtacgc cgtgtcgtcc gccgctaaca acaacggcag cgcctttgcc
     2461 ggattaagcg ccaactctcc gttctggaac tgtttactgg cgttctgcat gtttgtcggt
     2521 cgcttcgggg tgattatccc ggtgatggca attgccggtt cgctggtgag taaaaagagc
     2581 caacccgcca gctccggcac gctgccaacg cacggcccgc tgtttgttgg cctgttaatc
     2641 ggcaccgtgt tgctggttgg cgcactgacc tttatccctg ccctggcgct tggtccggtg
     2701 gcggaatatc tctcctgatg atattgagtg agcactgaat atgagtcgta aacaactggc
     2761 gctattcgaa ccaacacttg tcgttcaggc gctgaaagaa gcggtgaaaa aattaaaccc
     2821 gcaggcgcaa tggcgcaatc cggtgatgtt tatcgtctgg atcggcagtc tgctgaccac
     2881 ctgtattagc atcgcgatgg caagcggtgc gatgcctggc aatgcgctgt ttagcgcggc
     2941 aattagcggc tggctgtggg tcaccgtact gttcgctaat ttcgccgagg cgctggcaga
     3001 aggccgcagt aaagcgcagg ccaacagtct taaaggggtg aaaaaaactg cctttgaccg
     3061 caagctgcgt gagccgaaat atggcgctgc ggcggacaaa gttcctgccg atcaactgcg
     3121 taaaggtgac atcgtgctgg tagaagctgg cgatattatc ccctgcgatg gtgaagtcat
     3181 tgaaggcggc gcatcggtcg atgaaagcgc catcaccggg gaatcggcac cggtgatccg
     3241 tgaatccggc ggcgattttg cctccgtcac cggcggcacg cgtattcttt ctgactggct
     3301 ggtgattgag tgtagcgtta accccggtga gacatttctg gatcggatga tcgcgatggt
     3361 ggaaggcgca cagcgacgca aaacaccgaa cgagattgcc ctgactatcc tgctgattgc
     3421 tctgaccatc gtctttttac cggcaaccgc cacgctgtgg ccgttttccg cgtggggcgg
     3481 taatgccgtg agtgtaacgg tactggtggc gctgctggtc tgtctgatcc caaccactat
     3541 tggtggcctg ttgtcagcga tcggcgtcgc cgggatgagt cggatgctgg gcgcgaatgt
     3601 gattgccacc agcggacgtg cagttgaagc ggcaggtgac gttgacgttc tgctactgga
     3661 taaaaccggc accatcacac tcggtaaccg tcaggcgtcg gagtttatcc ccgcgcaggg
     3721 cgtggatgaa aaaacgctgg ctgacgccgc acaactggct tcgctggctg atgaaacgcc
     3781 ggaaggtcgc agtattgtga tcctcgccaa gcaacgtttt aacctgcgcg agcgcgatgt
     3841 gcagtcgctc catgccacct ttgtaccgtt tactgcgcaa agccggatga gcgggatcaa
     3901 catcgacaac cgcatgatcc gtaaaggttc tgtcgatgcc attcgccgcc atattgaggc
     3961 taacggcggt cacttccctg ccgatgttga tcaaaaagtc gatcaggttg cgcgtcaagg
     4021 agccacgccg ctggtggtgg tggaaggttc tcgtgtgctg ggcgttattg cgctgaaaga
     4081 tatcgtcaaa ggcggtatta aagagcgctt cgcccagctg cgcaaatggg cattaaaacg
     4141 gtgatgatta ccggcgataa ccgtctgact gccgccgcga tagctgcgga agcgggtgtc
     4201 gatgattttc tcgccgaagc gacaccggag gccaagctgg cattgatccg tcagtatcag
     4261 gcggaaggtc gtttggtagc gatgaccggc gacagcacca acgatgctcc ggcgctggcg
     4321 caggcagatg tcgcggtggc gatgaactcc ggcacccagg cggcgaaaga ggcgggcaat
     4381 atggtggatc tcgactctaa cccgaccaag ttgatcgagg tggtgcacat tggcaaacag
     4441 atgctgatga cccgtggctc gctgaccacc ttcagcattg ccaacgatgt ggcgaaatac
     4501 ttcgccatta ttccggcggc gttcgcggca acatatccgc agttaaatgc gctgaacatc
     4561 atgcgcctgc attcgcccga ctccgccatt ctcagtgcgg tcattttcaa cgccttgatt
     4621 atcgtctttt tgattcctct ggcgttaaaa ggcgtgagtt ataaaccgct taccgcttct
     4681 gccatgttgc gccgtaactt atggatttac ggtctgggtg ggctgctggt gccgtttatt
     4741 ggtatcaaag tcattgattt actgctaacc gtttgtagtc tggtgtgagg tttaccatga
     4801 gtggattacg tccggcatta tcgacatttc tctttctgtt attgattact ggcggcgttt
     4861 acccgctgct gaccaccgca ctggggcaat ggtggtatcc ctggcaggcc aacggttcgt
     4921 tgattcgtga aggtgatacg gtgcgcggtt cggcattaat cgggcaaaat tttaccggca
     4981 acggctattt tcatggtcgc ccgtcggcaa cagcagaaat gccctataat ccacaggctt
     5041 ctggcgggag caatctggca gtcagtaacc cggagctgga taaacagata gccgcacgcg
     5101 ttgccgcatt acgggccgct aacccggacg ccagcacgaa cgttccggtt gaactggtga
     5161 cggcgtcggc aagcggactg gacaataaca tcaccccgca agcggcggcc tggcagatcc
     5221 cacgcatcgc aaaagcgcgt aatctcagcg ttgaacagct cacgcagctg atcgcaaaat
     5281 acagccaaca accgctggtg aaatatatcg gccagccggt cgtcaacatt gtcgaactca
     5341 atctggcact ggataagctt gatgaataac gaacccttac gtcccgaccc cgaccgtctg
     5401 ctggaacaaa ctgctgcgcc gcatcggggg aagctgaaag ttttcttcag tgcctgtgca
     5461 ggcgtcggga agacctgggc gatgctggca gaagcccagc gactgcgggc gcaagggctg
     5521 gatattgtgg ttggtgtggt agaaacccat gggcggaaag ataccgccgc catgcttgaa
     5581 gggctggcta ttctgcctct gaaacgccag gcgtaccgtg ggcggcatat cagcgagttt
     5641 gatctcgatg ccgccctcgc ccgccgtccg gcgctgatct taatggacga actggcgcac
     5701 agtaatgcgc caggttctcg tcatcctaaa cgctggcagg atatcgaaga actgctggaa
     5761 gctggcattg atgttttcac aaccgtcaac gttcagcatc tggaaagtct gaatgatgtg
     5821 gtcagcggcg tcaccggaat tcaggtacgg gaaaccgtgc ccgatccttt tttcgatgcc
     5881 gccgacgacg tggtgctggt ggactagccc ccggacgatc tgcgccagcg gctgaaagaa
     5941 ggcaaagtct atattgccgg gcaggcggag cgcgccattg aacatttttt ccgcaaaggc
     6001 aatctgatcg ccctgcgcga actggcactg cgccgtactg ccgatcgcgt tgatgagcaa
     6061 atgcgcgcct ggcgggggca tcctggcgaa gagaaagtct ggcacacgcg cgacgcgatc
     6121 cttttatgca tcggccataa caccggcagc gaaaactggt ccgcgcggcg gcgcggctgg
     6181 cgtcacggct ggggagcgtc tggcacgcgg tgtatgttga accccctgcc ctgcatcgct
     6241 taccggagaa aaaacgtcgg gcaattctca gcgccttacg tctggcgcag gagctgggcg
     6301 cggagacggc aacactttct gatccagcag aagagaaagc ggtagtgcgt tatgcccgtg
     6361 aacataatct cggcaagatt attctcggtc gcccagcctc gcgccgctgg tggcgtcggg
     6421 aaacatttgc tgaccgactg gcgcgcatcg cccccgatct cgatcaggtg ctggtcgcgc
     6481 ttgatgaacc acccgcccgc acgattaaca acgcgccgga tagccgctca tttaaagaca
     6541 agtggcgggt acagattcag ggatgcgtgg ttgccgccgc gttatgcgcc gttatcacct
     6601 taattgccat gcagtggctg atggcgtttg atgccgccaa cctggtgatg ctgtatctgc
     6661 ttggcgtggt ggtggtggcg ctattttatg gacgctggcc ttcggtggtt gccaccgtca
     6721 ttaatgtggt gagtttcgat ctctttttta tcgccccacg cggcacgctc gccgtctctg
     6781 atgtgcaata tctgctgacc ttcgcggtga tgttaaccgt cgggctggtg atcgggaacc
     6841 ttactgctgg cgtgcgttat caggcgcggg tagcccgtta ccgcgagcaa cgcacacggc
     6901 acttatatga aatgtcgaaa gctctggcgg tgggccgcag tccgcaggac atcgccgcca
     6961 ccagcgaaca atttattgcc tctacgtttc atgcccgcag tcaggtgttg ttgcccgatg
     7021 acaacggtaa attgcagccg ttaacacatc cgcaaggaat gacgccatgg gacgatgcca
     7081 tcgcgcagtg gagttttgat aaaggcctgc ctgcgggcgc gggcaccgac acgttacccg
     7141 gtgtaccgta ccagattttg ccgctaaaaa gcggcgagaa aacctacggc tggtggtggt
     7201 ggagccgggg aatctgcgcc agttgatgat cccggaacag cagcgcctgc tggagacttt
     7261 tacgctgtta gtcgccaatg cccttgagcg gctgacgcta accgccagcg aagaacaggc
     7321 gcggatggca agcgaacgtg aacagatccg caacgccctg ctggcagcgc tttcgcatga
     7381 tttacgcacg ccgcttacgg tgctgtttgg tcaggcagaa atcttaacgc tcgatctggc
     7441 aagcgaagga tcaccccacg cccgccaggc cagcgagatt cgtcagcatg tgctgaacac
     7501 tacccgactg gtgaataatc tactggatat ggcgcgaatt cagtccggcg gctttaattt
     7561 gaagaaagag tggttaacgc tggaagaagt agtcggcagc gcgctgcaaa tgctggaacc
     7621 gggtttatcg tcgcccatca atctttctct gccagaaccg ctgaccttaa tccacgttga
     7681 cgggccactc tttgaacggg tgctgattaa tctgctggag aacgcggtga aatatgcggg
     7741 tgcgcaggcc gaaattggta tcgatgccca cgttgagggc gaaaatctac aactggatgt
     7801 ctgggataac ggccccggtc ttccgccagg ccaggagcag acgatatttg ataagtttgc
     7861 tcgcgggaat aaagagtcgg cggtgccggg tgtcgggctt gggttggcaa tttgtcgggc
     7921 gattgtggat gtacacggcg gcaccattac cgcgttcaac cgaccggaag gtggtgcctg
     7981 ttttcgtgtt acacttcccc agcaaaccgc tcctgaactt gaagactttc atgaggatat
     8041 gtgacaaacg ttctgattgt tgaagatgaa caggctattc gtagctttct gcgcacggcg
     8101 ctggagggcg acgggatgcg cgtctttgag gccgaaactc tgcaacgcgg cttgctggaa
     8161 gcggcaaccc gtaagccaga tttgattatt ctcgatctcg gcctgcccga tggtgatggg
     8221 attgagttta tccgcgacct gcgccagtgg agcccggtgc cggtgattgt gctttccgca
     8281 cgcagcgaag agagcgacaa gatcgccgcg ctggatgccg gagcggatga ttatctgagt
     8341 aagccgtttg gcattggcga attgcaggcc cgtctgcgcg tcgctttacg ccgccactcc
     8401 gccaccgccg cgcccgatcc gctggtgaaa ttttccgatg ttaccgtcga tttagccgcc
     8461 cgcgtgattc accggggtga ggaagaggtg catctcacac caattgagtt ccgcctgcta
     8521 gcggtgctgc tcaacaatgc cggaaaagta ctcacccagc gccagctcct taaccaggtg
     8581 tgggggccaa acgcggtcga acacagtcac tatttgcgta tttatatggg acatctgcga
     8641 caaaaactgg aacaggatcc ggcccggcct cgccacttca ttactgaaac cggtattggg
     8701 tatcggttta tgctttgaat tgttattcaa taaattcgct taaatagtga ttaatttatt
     8761 attaaacctg cgccgaatat tattatttgg caggtttaat ttcttaacgc aaatataaaa
     8821 cacgaaatta caacatttaa gtaacaaaac agcatctaat atggcgatat tcataattaa
     8881 tgatttatta tctggggtat gatcgttttt tgttgatctt cttcacagat tatagccatt
     8941 tcatggatag aataactcta ccttcaactg acacagcaag cggtaaaggt aaatggaaaa
     9001 taataaccgc ttaatgcctc atataaggcg gacaacccat atcatgaagt ttgcccatcg
     9061 taatagtttt gactttcatt tctttaatgc ccgctagtct tctgactaaa gggcacccca
     9121 acgtacaggt ctccctgact ttaagcatta caggttaata cctgtattcc tcggtgctca
     9181 tatactgcta acccttttaa actctaaata attcgagtcg cagcacttgc aacttgaggt
     9241 atgacgagta tagccagtta ccgggctggt ctgggttatt gcatctgcaa aaagcaaact
     9301 actgatttat ttatcagcgg tggagctttg ctttttttcc ggcgtgatcg atttctcctt
     9361 tgagaaattg aggacctgct attacctgaa ataaagagat gaataaaatg tcagaattaa
     9421 aaattgccgt tagccgttct tgcccggatt gtttttccac tcatcgtgca tgcgtgaata
     9481 ttgacgaaag taattatatt gacgttgccg ccattatttt atcagtc
//
LOCUS       DAETPZ010000125         9522 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.126, whole genome shotgun sequence.
ACCESSION   DAETPZ010000125 DAETPZ010000000
VERSION     DAETPZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.126"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(194..1447)
                     /locus_tag="K0W59_003610"
                     /pseudo
     CDS             complement(194..1447)
                     /locus_tag="K0W59_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1554..2447
                     /locus_tag="K0W59_003611"
     CDS             1554..2447
                     /locus_tag="K0W59_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3024484.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            2582..3802
                     /gene="mlc"
                     /locus_tag="K0W59_003612"
                     /gene_synonym="dgsA"
     CDS             2582..3802
                     /gene="mlc"
                     /locus_tag="K0W59_003612"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HBI3024485.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            3927..4622
                     /gene="bioD"
                     /locus_tag="K0W59_003613"
     CDS             3927..4622
                     /gene="bioD"
                     /locus_tag="K0W59_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBI3024486.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4575..5855)
                     /gene="clcB"
                     /locus_tag="K0W59_003614"
     CDS             complement(4575..5855)
                     /gene="clcB"
                     /locus_tag="K0W59_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HBI3024487.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYKVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGGLIVG
                     LLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVFTPTL
                     FIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEMTGEY
                     QLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6014..6628)
                     /gene="dmsD"
                     /locus_tag="K0W59_003615"
     CDS             complement(6014..6628)
                     /gene="dmsD"
                     /locus_tag="K0W59_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HBI3024488.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6671..7525)
                     /locus_tag="K0W59_003616"
     CDS             complement(6671..7525)
                     /locus_tag="K0W59_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBI3024489.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7527..8144)
                     /gene="dmsB"
                     /locus_tag="K0W59_003617"
     CDS             complement(7527..8144)
                     /gene="dmsB"
                     /locus_tag="K0W59_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBI3024490.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDNPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(8155..9279)
                     /locus_tag="K0W59_003618"
                     /pseudo
     CDS             complement(8155..9279)
                     /locus_tag="K0W59_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT         2473 a         2412 c         2461 g         2176 t
ORIGIN      
        1 tagaataact gcgcatcagt aataaatgac acacagcaaa atgaatccgt ttatttgggt
       61 acttataatc ctgatgacgc tagatgcgct gcgggaattg gctggcgctt cgtctatttt
      121 aggatggcta ttaacgttgg tttgagcagg caataagtcc ggacgggtat ttaccgcagt
      181 ccggacttat ttttcaggcg tgcagacgac gatgcaaacg cgccccgacc agcagagcaa
      241 tgaccagcat cagagcaata aatgcgccga cgccgttcca gccatagtta tgccagaaaa
      301 caccacccag cgtcccggca atactcgacc ccagatagta actgaacaga tacagcgagg
      361 aggcctggcc tttagcgcgt tttgcgcggg ggccgatcca gctgctggct actgagtggg
      421 ctgcgaagaa tccggctgag aagagtaaca ttccggcaaa gatcagccac agcgagctaa
      481 ataaggtcat cagtaaacca aacggcataa ctcccgtcga aaacaacatc accggaccgc
      541 gcccatagcg ggtggtcatg gttccggctt tgggtgagct ccatgtaccg gtcaaataag
      601 ccagcgataa taagccaacc acggcctgac tgacatgcta gggggaaagc atcaaccgat
      661 agccgatgta attaaacagc gtgacgaacg accccatcag caaaaagcct tctgcgaaca
      721 ataacggtaa tccccggtca cgccagtgca gacgaaagtt gataaataac gtcttagggc
      781 tcagcgaagt cgggcaaaaa tggcgtgatt cagggaggat tttccagaac atcaacgccg
      841 aggccagcgc gaaacaaccg attgccgcca gagcaattcg ccagttgaaa aagtccgtga
      901 agacaccact aattaagcgt ccgctcatgc cgccaattga gttgccgctg atatacaacc
      961 ccatcgaaaa ggccacgaaa ctggggtgga tttcctcgct aagataagtc atgccaacag
     1021 ctgccacgcc acttaacgaa agcccaatca aggcgcgcat aatcaaaatg ccgtgccagc
     1081 tggtcatcat tgtcgaaagt aacgtacaaa tggaggccaa cagtagcgcc gtgaccatca
     1141 ctggtttgcg accaatggca tcggatagcg ggccagtaaa tagcaaaccg atagccaaca
     1201 tcgccgtgga aatggacagt gaaatactac tgttcgcggg cgttaagcca aactcctgcg
     1261 aaagcaccgg aaggataggc tgcacacaat agagaagtgc aaaggttgcc agtccggcag
     1321 agaacagcgc cagggtgacg cgcataaatt gcggcgtacc gcgtttaata aattgatttg
     1381 gctgagaaat gctttgcttg tcagtgtcgc ttgccggagc gccatcaaca gttgtagtac
     1441 ggctcacttg aaatccttgc taaatatgcc tgtggatcag gcttatacat agggtaggaa
     1501 aatcgaattg ttctgtctaa tatattaata atctcaaata agatgtttta aatatgaata
     1561 ttgaacttcg tcatctgcgt tactttgttg ctgttgcgga agagctgcat tttgggcgcg
     1621 cggctgcccg cctgaatatt tcgcaaccgc cgctaagtca gcagattcag gcactggagc
     1681 aacaaatcgg tgcccgactg ctggcacgaa ctaatcgcag tgtattgctg acggcagcag
     1741 gaaaacagtt tcttgcagat agtcggcaaa tcctgtctat ggtggatgac gctgccgctc
     1801 gcgctgaaag actgcatcag ggtgaagcgg gggagttgcg tattggtttt acttcgtccg
     1861 ctccttttat tcgggcggtg tccgatacgt tatcgctgtt tcgccgtgat tatcctgatg
     1921 tccatttaca aacccgcgaa atgaacactc gcgagcaaat cgctccgctc attgaaggaa
     1981 cgctggatat gggattgctg cgtaacacag cgttaccgga gacgcttgaa cacgcagtca
     2041 tcgtccatga accgcttatg gcgatgatcc cgcacgatca tcccctggca aataatccga
     2101 gtgtaacact ggctgaactg gcgaaagaac cctttgtctt ttttgatccg cacgtcggga
     2161 ctgggctgta tgacgatatt ctcggactga tgcgacgtta ccatttgacg cccgtcatca
     2221 ctcaggaggt gggcgaggca atgaccatca tcggtctggt ttccgccggt ctgggtgttt
     2281 caattttgcc tgcgtcattt aaacgtgttc agctcaacga aatgcgctgg gtgccgattg
     2341 ctgaagagga tgcggtttct gaaatgtggt tggtctggcc gaaacatcat gaacaaagtc
     2401 cagctgcgcg taactttcgt attcatctgc tgaatgctct caggtgaggg aaatttcagc
     2461 gaaaaagctc gaaaaatgtg ctgttaatca catgcctaag taaaaatttg acgacacgta
     2521 ttgaagtgct tcaccatagc ctacagatta tttcggagcg cgaaaatata gggagtatgc
     2581 ggtggttgct gaaaaccagc ctgggcacat tgatcaaata aagcagacca acgcgggcgc
     2641 ggtttatcgc ctgattgatc agcttggtcc agtctcgcgt atcgatcttt cccgtctggc
     2701 gcagctggct cctgccagta tcactaaaat tgtccgtgag atgctcgaag cacacctggt
     2761 gcgagagctg gaaatcaaag aagcggggaa ccgtggccgt ccggcggtgg ggctggtggt
     2821 tgaaactgaa gcctggcact atctttctct gcgcattagt cgcggggaga ttttccttgc
     2881 tctgcgcgat ctgagcagca aactggtggt ggaagagtcg caggaactgg cgttaaaaga
     2941 tgacttgcca ttgctggatc gtatcatttc ccatatcgat cagtttttta tccgccacca
     3001 gaaaaaactt gagcgtctaa cttcgattgc cataaccctg ccgggaatta ttgatacgga
     3061 aaatggtatt gtacatcgca tgccgttcta cgaggatgta aaagagatgc cgctcggcga
     3121 ggcgctggag cagcataccg gcgtaccggt ttatattcag catgatatca gcgcatggac
     3181 gatggcagag gccttgtttg gtgcctcacg cggggcgcgc gatgtgattc aggtggttat
     3241 cgatcacaac gtgggggtgg gcgtcattac cgatggtcat ctgctacacg caggcagcag
     3301 tagtctcgtg gaaataggcc acacacaggt cgacccatat ggtaaacgct gttattgcgg
     3361 gaaccacggc tgcctcgaaa ccatcgccag tgtggacagt attcttgagc tggcacagct
     3421 gcgtctcaat caatccatga gctcgatgtt acatgggcaa ccgttaaccg tggactcatt
     3481 gtgtcaggcg gcattgcgcg gcgatctact ggcaaaagac atcattatcg gggtgggcgc
     3541 gcatgtaggg cgcattcttg ccatcatggt gaatttattt aacccacaaa aaatactgat
     3601 tggctcaccg ttaagtaaag cggcagatat cctcttcccg gtcatctcag acagcatccg
     3661 tcagcaggcc cttcctgcgt atagtcagca cattagcgtt gagagtactc agttttctaa
     3721 ccagggcacg atggcaggcg ctgcactggt aaaagacgcg atgtataacg gttctttgtt
     3781 gattcgtctg ttgcagggtt aacatttttt aactgttcta ccaaaatttg cgctatctca
     3841 atttgggcca ggaaagcata acttagactt tcaaggttaa ttattttcct agtttatatt
     3901 tgtgaagcat aacggtggag ttagtgatgc tgaagcgttt ctttattacc ggtacagaca
     3961 cttctgtagg gaaaacggtg gtttcccgcg cattgctaca agcgttagcc tcccagggaa
     4021 aaacggttgc gggatataaa cccgtagcga aggggagcaa agagacaccc gaagggctgc
     4081 gtaataaaga tgccttggtg ttgcagagtg tttcaaccat cgaactgcct tatgaagcag
     4141 ttaatcctat cgcgttaagc gaagaagaaa gtagcgtggc gcacagttgc ccaatcaatt
     4201 acaccctcat ttcaaacggc ctggcaaacc tgaccgataa agtcgatcat gtcgtggtag
     4261 aagggactgg cggctggcgc agtctgatga atgatttgcg tccactctct gaatgggtag
     4321 tgcaggaaca actgccggtg ttgatggttg tcggtattca ggaaggttgc attaaccatg
     4381 cactgctaac agctcaggcg atcgccaacg acgggctgcc gctcattggc tgggtggcta
     4441 accgaatcaa cccaggactg gcgcattatg cggaaatcat tgatgtgctg ggtaaaaaac
     4501 ttccggcacc gctcattggt gaactgcctt atctgccgcg cgccgaacag cgtgaactgg
     4561 ggcaatacat ccgcttagct atgctgcgca gtgtgctggc ggtagataga gtcacggtgt
     4621 aacgtccgcg aaattaccga cgcaattacg caggcaatca ataaaccggg gagtagctga
     4681 tactccccgg tcatttcaca tatcatcaac gtcgacataa tcggcgcgtg cgtggttgcc
     4741 gccaacagtg tcgccatccc ggtcaatccg agtaaaagcg taatttcttc gccatcaggg
     4801 aaccataatc ccaggctacg accatacaac atgccaatgg caagaccgat aaatagcgtc
     4861 ggtgtaaaga caccaccggg tgcgccggaa ccgctactcg ccagcacggc acacagttta
     4921 caaaggaaga tcccggcaat gatcattaac agtggtgggg cggttaaaaa ggattgtacg
     4981 gtgctatagc cgttgcccca cactgcaggt gtgaacaggg aaagcagacc cacgatcaac
     5041 ccgcctagtg ccagttgcca gggcggcgca agtttgagac tcacaaatcc acgatgacag
     5101 gcgttcatta acgttaacaa cagtggtccg cacagacctg ccagcacacc tgtactgata
     5161 atcagcgcat agtcacgagc ctgaaccgtc actttgtaga gtaacgcgtc gctatgatta
     5221 atcagattgc taaccagcaa tgccacgacg gcggaaataa tcaccgggcc gagagaggcc
     5281 aacatcatag tgccaaacag cacttcggct ataaataaac tgccagcaag cggggcacga
     5341 taggccgcag ccattcccgc cgcggcccca caggcgatcc ataatttcca ttcctggcgt
     5401 ggcgtaaaac gttgggcaaa acaggaggcg gcaagggcag ctaaaagaat catcgcacct
     5461 tcgcgaccaa ttgcactgcc gctggttact accagcagcg aggcaagcga tttaaccagg
     5521 cttgctgcgt aatcgaactg tccatcggtt tgcaacgctt ccatgtaatc ggtcggcgca
     5581 tgagggcgtt gttgggtaaa tttctgccag cccatcagca acaaacccgc cgccagtccg
     5641 ccgagcgccg gagttagcag ccgtcgccag ggggaaaggt ttgtcgctgc attgaccaga
     5701 ctgccggagt cattattgag gaacaaccac tccagtagca gcatcgcatg acgaaacccg
     5761 gcaacggcaa agacagcgag aataccgacg actgttgcga taagcagacg gcggaacata
     5821 gtgcgcagat ccggatagat gtgtagatga tgcatgagca gaaatgggca atgaaacacg
     5881 ggcgactatt ttgcggcaaa agaggccaac cgcaaaagca gtgcgtgctt taagagataa
     5941 aaaaacgtga cattgtaacg tattattgcc gaatcgcgcg taatcgtcac catccggcaa
     6001 tattacggtg atcctatcga aagagtggtt taaccgcgac aggaatcaac agttgtgatt
     6061 gccattgcgc cagcgttaag cgagccagtt cacccagtgc acggtagaaa gggtgttctg
     6121 ctttttcgat aaaaacatca agaaaacgtg ttgaccacgg aaaaaggtgc catgccagca
     6181 attcttcgca ttctgtctgg cgaccattct ccgccaacca cgcagccatc aacagcagtg
     6241 aaccaaaatg atcttccggt tcgttttgct tcatttcaaa ctgaatgcct ttctcgcgca
     6301 tccactgacg aagtgccaat gttgaatcgc caaacagcac agattcgcga tccagccaga
     6361 ccgaccccca cggcggagac ggcagtgccc acgggccgac aaacaaacgc tgccaggcct
     6421 gggcgtgagt ctcttcacac tgtgtctgaa aagccgtcac cagcggcgct aatgacgctt
     6481 ctggtagagg ccactgagtt tcccagccgt cactggtgag taccgcaacc agaggtgcgg
     6541 cttccgcact ctctggagcg taataaaaca gcgctcccaa cacgcgcgcc gcgacagaaa
     6601 aattatcttg ctgtgaaaaa tgggtcattc ctaacatcct gaaatgtgcg ggttgccccg
     6661 caccaatttg ttaacctgca atagccattc ctactgtcat gtgcaggcca taaaagagca
     6721 cacgcccgat catctcacca acaagtatga ggattagccc cagaacaagt ccggcaacgt
     6781 gaggttcacg gcgacgaatg agcggacaaa gccagcatcc aagacctgcg cataacaata
     6841 ccacacgcca gacctgtaag gaggcgtaat ctggtaccag tgcgctggct tgttgcacgg
     6901 atgagtgaat agacgccaga gacaaacctt gcagcacaat cacctcggca caagcgatca
     6961 gcgccagaac gctaatgatg gcaaatggtg tggtattaaa tgtaacgcgg gctgcccgca
     7021 gaattgcggc agcaagtatt ggaccgctca acaataccgt caggaagaaa gccagcgtag
     7081 tgtaaccgtt atgccaggtt ggcacggtgt cgatttgata cacgcaggtc atcatccaga
     7141 cgaaaatgac gccgagcgcc atgctgacaa gtaaccagag tttccccaac gcttgcggca
     7201 ttttaccgat gaccgccacc agccaccata gtccgccaac ggcaaaaaat atggaaccgg
     7261 cagcaatctc gttactcaga ccagaagctc cgattcgatt cagcgagtta aacgctcgca
     7321 gtggcgatcc caggtgcatg acagaggcaa taaacccgat gcccatcaac agccagagga
     7381 aaaacatgcc acgaacaatg tgctgacggt ctgcgtcatt tttggcggcg aaccagccaa
     7441 ttccgctaac aatcagcgcg cctactacgc attgacccaa tacggtaaag ataaccagtg
     7501 gccattcatg ccatccattt cccattttac acttcctccg gattagccag ataaccagtg
     7561 gtatcgccgg tcgggcggct gttggcgtta ggtttgataa cgatattggg ttttgtgaaa
     7621 tgcgcgcgcg gcagtggcgc gacggcagcc agagtaccgt gcttctggcg cagctcttca
     7681 attggaccga actcaagtgc acgcagcggg caggattcga cgcatatggg ttgtttcccc
     7741 tcggcgacgc gcgaataaca accatcgcac ttcgtcatgt gccctttttc agcattgtac
     7801 tgtggtgcgc cgtacggaca agccatgtgg cagtagcggc agccgataca aacatcttca
     7861 tcaaccacca caaagccatc ttcacgctta tgcattgcgc cgctggggca gacttttgta
     7921 catgcggggt tatcgcaatg gttacaggag atggagagat aataagcgaa cacgttctgg
     7981 tgccagatgc cattatcttc ctgccagtcg ccgccagcat attcataaat acggcggaaa
     8041 ctgacttccg ggccaagatc tttaaaatct ttgcacgcca gttcgcaggt tttacagcca
     8101 gtacagcggc tggaatcgat aaaaaatcca tattgtgtgg tcatgggcta ctccttaaac
     8161 cttttcgatc tggacaagat tgctgtgcga cgggtttccc tttgctagcg gtgaagggcg
     8221 gtgagaggtc agaatattga tactgccgcc gtgatcgacc cggtcaccaa acatatccgc
     8281 tttaagccac gcaccttgcc cgatggcggt aacgccaggc agaatacgcg gagtcacttt
     8341 tgcggcaatc agcatttctc cattattgtt aaatacccgc acggtatcgc catgacggat
     8401 accgcgtgcc tgagcatcaa tggggttgat ccacacctct tgtgggcagg cctgctgtaa
     8461 cacatcaata ttgccgtagc tggagtgggt acgcgctttg tagtggaagc ccgttaactg
     8521 cagtggatag gttttccgca ggggagcgtc ccagccatca aaacctgagg tatacgcagg
     8581 aatgggatga ataatttcat cttttttcaa ttcccaggta tctgcaatct tcgccagtcg
     8641 ttcagaataa atttcgattt tccccgaagg tgttttcaac gggtttgcct gtggatcttc
     8701 acggaatgcg cggaaagcga cgtagtgttc ttccgggcat tttttcttaa agatcccggt
     8761 cgttttcatc tcctcgtagt cgggcatctc agggttacgt tccttcgttt tcgcatggag
     8821 atatttgatc cattcatgct gactgcgacc ttcagtaaag gtttgataaa cgtctggtcc
     8881 taagcgtttg gcgacttcac tcagcatcca gtagatgggt ttgcgttcaa attttgctga
     8941 ggttgcgggt tgggcgagga tcacatagcc catattccct gcagattcat gagagataag
     9001 gtcttcttgc tctgttggca tcaggtcagg caacaggata tcgcaatact tagccgaggc
     9061 cgtcatgaag tggtcaatgc caacaatcat ctcgcacttg ctgtcatcct gaagcacctc
     9121 atgggtgtga ttgatgtcgc catgttgatt gatcaatgtg ttactggcgt agcaccataa
     9181 aaacttgatg gggacatcca gtttttcttt tccacgaaca ccatcacggg tcgcggtcat
     9241 tttcgtacca tggtcgatgg catctgtcca tgtaaacatc tggggtcctt acagctcgtc
     9301 ctgacatgcg gtctcgcctt atatgcacat ggagaacatg gtcactccag accaacacta
     9361 atcaacatta accctattca ctttcgttaa gcactttttg gtgggtccgt ttggatgcat
     9421 tgaataggtg cggactccac atggagacag aaagtgcttt accaaaggag catcctttat
     9481 gaataccaac gaagttagcg tcatcaacct tgaccagctt gt
//
LOCUS       DAETPZ010000126         9163 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.127, whole genome shotgun sequence.
ACCESSION   DAETPZ010000126 DAETPZ010000000
VERSION     DAETPZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.127"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..926
                     /locus_tag="K0W59_003619"
                     /pseudo
     CDS             1..926
                     /locus_tag="K0W59_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
     gene            1178..2095
                     /gene="oxyR"
                     /locus_tag="K0W59_003620"
     CDS             1178..2095
                     /gene="oxyR"
                     /locus_tag="K0W59_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HBI3024491.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2078..3478)
                     /gene="sthA"
                     /locus_tag="K0W59_003621"
     CDS             complement(2078..3478)
                     /gene="sthA"
                     /locus_tag="K0W59_003621"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HBI3024492.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCKILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3799..4449
                     /gene="fabR"
                     /locus_tag="K0W59_003622"
     CDS             3799..4449
                     /gene="fabR"
                     /locus_tag="K0W59_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HBI3024493.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4449..4808
                     /locus_tag="K0W59_003623"
     CDS             4449..4808
                     /locus_tag="K0W59_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HBI3024494.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4848..5948)
                     /gene="trmA"
                     /locus_tag="K0W59_003624"
     CDS             complement(4848..5948)
                     /gene="trmA"
                     /locus_tag="K0W59_003624"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HBI3024495.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6062..6252
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6317..8161
                     /gene="btuB"
                     /locus_tag="K0W59_003625"
     CDS             6317..8161
                     /gene="btuB"
                     /locus_tag="K0W59_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HBI3024496.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNCTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8106..8963
                     /gene="murI"
                     /locus_tag="K0W59_003626"
     CDS             8106..8963
                     /gene="murI"
                     /locus_tag="K0W59_003626"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBI3024497.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2097 a         2219 c         2469 g         2378 t
ORIGIN      
        1 gtctgggtat tggcgaagcg ccatttatgc ctgcgggagt aaagtcgatc accgactgat
       61 atgcacaaaa agaacgcggc acggcgctgg ggatttttaa ctcatctacc gttatcggtc
      121 aggctatcgc gcctcctgct cttgtattga tgcagctggc atggggctgg cggacgatgt
      181 tcgttatcat cggcgtggca gggattctgg ttgggatctg ttggtacgcg tggtatcgca
      241 accgggcgca gtttgtcctg actgacgaag aacggacgta tctctccgcg ccggttaaac
      301 cgcgtccaca gctgcaattt agcgagtggc tggcgctgtt taagcatcgg acaacctggg
      361 ggatgatttt aggtttctct ggcgtcaatt ataccggttg gctctacatt gcgtggttac
      421 ccggttattt gcaagccgaa caaggtttca gtctggcgaa aaccggttgg gtagcggcga
      481 ttcctttcct cgcggcggcg gtcgggatgt gggttaacgg tattgtggtc gatcggctgg
      541 cgaaaaaagg ctacgacctg gcgaagacgc gtaaaacggc tattgtctgc ggtttgatga
      601 tgtcggcatt aggcacgttg ctggtcgtgc aatcttcctc gccagctcag gcggtagcgt
      661 ttatctcaat ggcgctgttc tgcgtgcatt tcgcgggaac gtctgcatgg ggactggtgc
      721 aggtgatggt gtcagaaaca aaagtggctt ccatcgccgg gattcaaaac tttggcagtt
      781 ttgtttttgc ttcctttgct ccgatcgtaa ccggttgggt agtggatacc acacactcgt
      841 ttaatctggc gctggttatc gcggcctgtg tgacgttcac cggggcgctg tgttacttct
      901 ttattgtcaa agatcgcatt gagtaatcgt tactatcagg ctctcttgct gtgggcctgt
      961 agaataaaaa aaagcggatc ctggagatcc gcaaaagttc acgttggctt tagttattcg
     1021 agttgagaaa ctctcgaaac gggcagtgac ttcaagagtt aaaagaggtg ccgctccgtt
     1081 tctgtgagca attatcagtc agaatgcttg atagggataa tcgttcattg ctattctacc
     1141 tatcgccatg aactatcgtg gcgatggagg atggataatg aatattcgtg atcttgagta
     1201 cctggtggca ttggctgaac accgccattt tcggcgtgcg gcagattcct gccacgttag
     1261 ccagccgacg cttagcggcc aaattcgtaa gctggaagat gagctgggcg tgatgttgct
     1321 ggagcggacc agccgtaaag tgttgttcac ccaggcggga atgctgctgg tggatcaggc
     1381 gcgtaccgtg ctgcgtgagg tgaaagtcct taaagagatg gcaagccagc agggtgagac
     1441 gatgtccgga ccgctgcata ttggtttgat tcctacggtt ggaccatacc tgctaccgca
     1501 tattatcccg atgctgcacc agacatttcc aaagctggaa atgtatctgc atgaagcaca
     1561 gacccaccag ttactggcgc aactggacag cggcaaactc gattgcgtga tcctcgcgct
     1621 ggtgaaagag agcgaagcat tcattgaagt gccgttgttt gatgagccaa tgttgctggc
     1681 tatctatgaa gatcacccgt gggcgaaccg cgaatgcgta ctgatggccg atctggcagg
     1741 ggaaaaactg ctgatgctgg aagatggtca ctgtttgcgc gatcaggcaa tgggtttctg
     1801 ctttgaagcc ggggcggatg aagatacaca cttccgcgcg accagcctgg aaactctgcg
     1861 caacatggtg gcggcaggta gcgggatcac tttattgcca gcgctggctg tgccgccgga
     1921 gcgcaaacgc gatggggttg tttatctgcc atgcattaag ccggaaccac gccgcactat
     1981 tggcctggtt tatcgtcctg gctcaccgct gcgcagccgc tatgagcagc tggcagaggc
     2041 catccgcgca agaatggatg gccatttcga taaagtgtta aaacaggcgg tttaaaccgt
     2101 ttaacgcagc tacccgatag gcttccgcca tcgtcgggta gttaaaggtg gtgttgacga
     2161 agtactcaat agtgttgccg ccacctttct gttccataat cgcctgaccg atatgaataa
     2221 tttcggcagc gcgctcgcca aagcagtgaa tacccagaat ctcttttgtt tcccgatgga
     2281 acaaaatttt cagcgtgccc acgttcatgc cgacgatttg tgcacgtgcc agatgtttga
     2341 actgggcgcg gcccacttca tacggcactt tcatcgcggt cagctgctgt tcggttttgc
     2401 ccacagagct gatttccggg atggtgtaaa tgccggtagg gatatcttca atcagatgtg
     2461 cggtggcttc gccttttacc agcgcctgcg cggcaatgcg cccctggtca taggccgctg
     2521 atgccaggct cggataacca atcacgtcgc ccaccgcgta aacgtgcggc tgtgcggtct
     2581 gatacatgct gttgaccttc agctgtccgc gactgtcagt ttccagccca atgttctgta
     2641 acgccagcga atcggtatta ccggtacgac cgttggcata gagcaggcag tcagctttca
     2701 gttttttacc cgacttcaga tgcatgatca caccgtcgtc acagctttcg atcttctcgt
     2761 actcttcgtt gtgacgaatc actacgccac tgttccagaa gtgataggag agagaatctg
     2821 acatctcttg atcgagaaat gccagcaggc gatcgcgggt gttgatcaga tccactttta
     2881 catccatacc gcggaagatc gacgcatatt cacagccgat cactccagca ccatagataa
     2941 gtacatggcg cggttcgtgg tgcatgctaa gaattgagtc gctgtcgtaa atgcgtggat
     3001 gggtgaaatc aacatctgtt ggatgatatg gacgagagcc gcaggcaata acaaattttt
     3061 cagcggttag tgtttcaacg ctgccgtccg ggcaatccag cgccaacgta tgctcgtcaa
     3121 caaagcgagc gttcccctgc aatattttac agtgattacg ttcgtaaaat ccctgacgca
     3181 tgcgcgtttg ttgattaatc acgttatcgg catggttaag gatatcggca aaagaagagc
     3241 ggagcagtcg ggaatggtcg ctgtatagtg ggttttgatt gaattctata atgcggctga
     3301 cggcgtgacg gagagctttc gacgggatgg tgccccagtg ggtgcaaccg ccgccaacat
     3361 tttgataacg ctcgataact gcgacgcgcg caccttgctt aaccaggccc attgcagcgc
     3421 cttcgccgcc ggggccggaa cctattacta tggcatcgta atcgtaggaa tgtggcatgg
     3481 tagggcttac ctgttcttat acataaaatc aacataatgg taacattcta tcgcgggtaa
     3541 gccaattgat ccccgtcatt tatctggcta tatcctgagc ggcctttgct ttgtctgttt
     3601 cttacttttg ccctgacgtt ttattggatt tttatcgacg atactctccg tttaagcggc
     3661 aggtttccgc tgtacgtaaa agaaccggcc agtaaatatg ttttattgcg ttaccgttca
     3721 ttcacaatac tggagcaatc cagtatgttc attctctggt atagtgccag cagtactttt
     3781 ggcaaggatt cagacatcgt gatgggcgta agagcgcaac aaaaagaaaa aacccgccgt
     3841 tcgctggtgg aagccgcatt tagccaatta agtgctgaac gcagcttcgc cagcctgagt
     3901 ttgcgtgaag tggcgcgtga agcgggcatt gctcccacct ctttttatcg gcatttccgc
     3961 gacgtagacg aactgggtct gaccatggtt gatgagagcg gtttaatgct acgccaactc
     4021 atgcgccagg cacgtcagcg tatcgccaaa ggcgggagtg tgatccgtac ctcggtctcc
     4081 acatttatgg agttcatcgg taataatcct aacgccttcc ggttattatt gcgggaacgc
     4141 tccggcacct ccgctgcgtt tcgtgccgcc gttgcgcgtg aaattcagca cttcattgcg
     4201 gaacttgcgg actatctgga actcgaaaac catatgccgc gtgcgtttac tgaagcgcaa
     4261 gccgaagcaa tggtgacaat tgtcttcagt gcgggtgccg aggcgttgga cgtcggcgtc
     4321 gaacaacgtc ggcaattaga agagcgactg gtactgcaac tgcgaatgat ttcgaaaggg
     4381 gcttattact ggtatcgccg tgaacaagag aaaaccgcaa ttattccggg aaatgtgaag
     4441 gacgagtaat gaaacaagca aatcaagata gaggtacgct gctgctggcg ttggttgctg
     4501 gcttatcgat taatggtact ttcgcagcgc tgtttagctc cattgtgcca ttttctgtat
     4561 tcccgattat ttccctggtg ctgacggttt actgcctgca tcaacgttat cttaatcgca
     4621 ccatgtcggt aggcttgccg ggtctggcag ctgcctgttt tattctcggc gtactgctgt
     4681 acagcacggt agttcgtgcg gaatatccgg atatcggctc taacttcttc ccggcagtac
     4741 tctccgtcat tatggtgttc tggattggcg cgaagatgcg taaccgtaag caggaagttg
     4801 ctgagtaatc ggtatcatgc cggatgctgg cgcatccggc atgggtttta cttcgcggtc
     4861 agtaatacgc cgcactccat atggtgcgtg taggggaact gatcaaacag agccagacgt
     4921 tcgaccttgt gcgtctggct taatgtttcc agatttttgc ataacgtttc cggattgcag
     4981 gagatataca gaatacgtgg atacgcctgc accattttct cggtttcact gtccagaccg
     5041 ctgcgcggtg ggtcgacaaa aatggtttcg aactgatagc tctttaagtc gatcccttgc
     5101 aggcggttaa actcgcgcac accattcatc gcctgagtaa attcttctgc cgccatacga
     5161 ataatttgta cgttatcaat atggttagcg gcaatgttgt attgcgcggc agcgactgac
     5221 ggtttggcga tttcggtggc taatacccga tcaaaattgc gcgccagcgc taatgaaaag
     5281 ttaccgttgc cgcagtacag ctccagtaaa tcgccttttg agcctttggt tacgtccagc
     5341 gcccattcca gcatttgaat attcatcgcc gcgttcggct gggtaaagct gttttctacc
     5401 tgacgataga tcatctcttt ccctgcgacc ggcagacgct cgtcgatgta atcttgatcc
     5461 agcgcgattt tggttttcgt tgcccgacca atcagatgca cattcagatt ctgcgcgcgc
     5521 agtgcatcgc gcagtgcttc cgcttcctga cgccactcat catccagctt tttatggtac
     5581 agcagggaaa ccaccgcctg attactcagc gtggtgaggt aatcaatctg gaacaacttg
     5641 tggcgcagaa cgggattatt acgcacaccc gcaatcatcg ccgtcatcaa ctggttgatg
     5701 agttcactgg cggcggggaa gctatccacg cggatgcggc ttttggtttg ttgatcgaaa
     5761 atgatgtgat acaggtcatc gccatcgtgc cagatgcgga actccgcgcg catccggtaa
     5821 tgactgaccg gcgagcgaaa cacttccgga accaggtcag aaaacggtgc catcatactt
     5881 tgcaaacgta ccactttttc ggctaactgc gcttcatact gttctgttgg aaggtgttcg
     5941 ggggtcatga tgtatcctga aaaattaaag tacgcggcga ttgtagggat tgctcatcag
     6001 atgtccagat cttgatgaat tcctatttgt gagctacgtc tggacagtaa cttgttacaa
     6061 cctgtagcat ccacttgccg gtcctgtgag ttaataggga atccagtgcg aatctagagc
     6121 tgacgcgcag cggtaaggaa aggtgcgatg attgcgttat gcggacactg ccatccggtg
     6181 ggaagtcatc atctcttagt atcttagata cccctccaag cccgaagacc tgccggccaa
     6241 cgtcgcatct ggttctcatc atcgcgtaat attgatgaaa cctgcggcat ccttcttcta
     6301 ttgtggatgc tttacaatga ttaaaaaagc ttcgttgctg acggcgtgtt ccgtcacggc
     6361 attttccgct tgggcacagg ataccagccc ggatactctc gtcgttactg ctatccgttt
     6421 tgaacagccg cgcagcactg tgcttgcacc aaccaccgtt gtgacccgtc aggatatcga
     6481 ccgctggcag tcgacctcgg tcaatgatgt gctgcgccgt cttccgggcg tcgatatcac
     6541 ccaaaacggc ggttcaggtc agctctcatc tatttttatt cgcggtacaa atgccagtca
     6601 tgtgctggtg ttaattgatg gcgtccgcct gaatctggcg ggggtgagtg gttctgccga
     6661 ccttagccag ttccctattg cgcttgtcca gcgtattgaa tatatccgtg gaccgcgctc
     6721 cgctgtttat ggttccgatg caataggcgg ggtggtgaat atcatcacga cgcgcgatga
     6781 acccggaacg gaaatttcag cagggtgggg aagcaatagt tatcagaact atgatgtctc
     6841 tacgcagcaa caactggggg ataaaacacg ggtaacgctg ttgggcgatt atgcccatac
     6901 tcatggttat gatgttgttg cctatggtaa taccggaacg caagcgcaga cagataacga
     6961 tggtttttta agtaaaacgc tttatggggc gctggagcat aactgtactg atgcctggag
     7021 cggctttgtt cgcggctatg gctatgataa ccgtaccaat tatgacgcgt attattctcc
     7081 cggttcaccg ttgctcgata cccgtaaact ctatagccaa agttgggacg ccgggctgcg
     7141 ctataacggc gaactgatta aatcacaact cattaccagc tatagccata gcaaagatta
     7201 caactacgat ccccattatg gtcgttatga ttcgtcggcg acgctcgatg agatgaagca
     7261 atacaccgtc cagtgggcaa acaatgtcat cgttggtcac ggtagtattg gtgcgggtgt
     7321 cgactggcag aaacagacta cgacgccggg tacaggttat gttgaggatg gatatgatca
     7381 acgtaatacc ggcatctatc tgaccgggct gcaacaagtc ggcgatttta cctttgaagg
     7441 cgcagcacgc agcgacgata actcacagtt tggtcgtcat ggaacctggc aaaccagcgc
     7501 cggttgggaa ttcatcgaag gttatcgctt cattgcttcc tacgggacat cttataaggc
     7561 accaaatctg gggcaactgt atggcttcta cggaaatccg aatctggacc cggagaaaag
     7621 caaacagtgg gaaggcgcgt ttgaaggctt aaccgctggg gtgaactggc gtatttccgg
     7681 atatcgtaac gatgtcagtg acttgatcga ttatgatgat cacaccctga aatattacaa
     7741 cgaagggaaa gcgcggatta agggcgtcga ggcgacagcc aattttgata ccggaccact
     7801 gacgcatact gtgagttatg attatgtcga tgcgcgcaat gcgattaccg acacgccgtt
     7861 gttacgccgt gctaaacagc aggtgaaata ccagctcgac tggcagttgt atgacttcga
     7921 ctggggtatt acttatcagt atttaggcac tcgctatgat aaggattact catcttatcc
     7981 ttatcaaacc gttaaaatgg gcggtgtgag cttgtgggat cttgcggttg cgtatccggt
     8041 cacctctcac ctgacagttc gtggtaaaat agccaacctg ttcgacaaag attatgagac
     8101 agtctatggc taccaaactg caggacggga atacaccttg tctggcagct acaccttctg
     8161 aaccacgtcc caccgtgctg gtgtttgact ccggcgtcgg tgggttgtcg gtctatgacg
     8221 agatccggca tctcttaccg gatctccatt acatttatgc tttcgataac gtcgctttcc
     8281 cgtatggcga aaaaagcgaa gcgtttattg ttgagcgagt ggtggcaatt gtcaccgcgg
     8341 tgcaagaacg ttatcccctt gcgctggctg tggtcgcttg caacaccgcc agtaccgttt
     8401 cacttcctgc attacgcgaa aagttcgact tcccggttgt tggtgtcgtg ccggcgatta
     8461 aacctgctgc acgtctgacg gcaaatggca ttgtcggatt actggcaacc cgcggaacag
     8521 ttaaacgttc ttatactcat gagctgatcg cgcgtttcgc taatgaatgc cagatagaaa
     8581 tgctgggctc ggcagagatg gttgagttgg ctgaagcgaa gctgcatggc gaagatgttt
     8641 ctctggatgc actaaaacgt atcctacgcc cgtggttaag aatgaaagag ccgccagata
     8701 ccgttgtatt gggttgcacc catttccctc tactacaaga agaactgtta caagtgctgc
     8761 cagagggaac ccggctggtg gattctggcg cagcgattgc tcgccgaacg gcctggttgt
     8821 tagaacatga agccccggat gcaaaatctg ccgatgcgaa tattgccttt tgtatggcaa
     8881 tgacgccaga agctgaacaa ttattgcccg ttttacagcg ttacggcttc gaaacgctcg
     8941 aaaaactggc agttttaggc tgatttggtt gaatgttgcg cggtcggaaa attattttaa
     9001 atttcctctt gtcaggccgg aataactccc tataatgcgc caccactgac acggaacaac
     9061 ggcaaacacg ccgacgggtc agcggggttc tcctgagaat tccggcagag aaagcaaaaa
     9121 taaatgcttg actctgtagc gggaaagcgt attatgcaca cct
//
LOCUS       DAETPZ010000127         9079 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.128, whole genome shotgun sequence.
ACCESSION   DAETPZ010000127 DAETPZ010000000
VERSION     DAETPZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.128"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(227..2770)
                     /gene="mdoH"
                     /locus_tag="K0W59_003627"
     CDS             complement(227..2770)
                     /gene="mdoH"
                     /locus_tag="K0W59_003627"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HBI3024498.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKLWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2763..4298)
                     /gene="mdoG"
                     /locus_tag="K0W59_003628"
     CDS             complement(2763..4298)
                     /gene="mdoG"
                     /locus_tag="K0W59_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HBI3024499.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4692..5849
                     /gene="mdoC"
                     /locus_tag="K0W59_003629"
     CDS             4692..5849
                     /gene="mdoC"
                     /locus_tag="K0W59_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HBI3024500.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5857..7338)
                     /gene="clsC"
                     /locus_tag="K0W59_003630"
                     /pseudo
     CDS             complement(5857..7338)
                     /gene="clsC"
                     /locus_tag="K0W59_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
     gene            complement(7280..7813)
                     /gene="ymdB"
                     /locus_tag="K0W59_003631"
     CDS             complement(7280..7813)
                     /gene="ymdB"
                     /locus_tag="K0W59_003631"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HBI3024501.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7908..8219)
                     /locus_tag="K0W59_003632"
                     /pseudo
     CDS             complement(7908..8219)
                     /locus_tag="K0W59_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            complement(8340..8672)
                     /gene="csgC"
                     /locus_tag="K0W59_003633"
     CDS             complement(8340..8672)
                     /gene="csgC"
                     /locus_tag="K0W59_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HBI3024502.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQFCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8731..>9079)
                     /gene="csgA"
                     /locus_tag="K0W59_003634"
     CDS             complement(8731..>9079)
                     /gene="csgA"
                     /locus_tag="K0W59_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HBI3024503.1"
                     /translation="NNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGAD
                     VGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGATVDQTASNSSVNVT
                     QVGFGNNATAHQY"
BASE COUNT         2280 a         2347 c         2157 g         2295 t
ORIGIN      
        1 gagagtggta aaggagaggc cctggaaata ccccacagcc ccgaaaaaga gccagtcagt
       61 tgcttaactg actggcatta cggcataacg ccggtatttt tattggctcg ttgtttttgc
      121 cggatgtggc gtgaacgcct tatccgacct acaaaatctt gcaaaatcaa taaattgcag
      181 gaacgatgta ggcctgataa gcgtagcgca tcaggcaact acgtttttat tgcgaagccg
      241 catccggttt acgcaatgcc agtggattga gctttatccc ttcgtaataa ctcacccatg
      301 aagaatatct ttcaggggaa ttccagacgc ggaaatgcag acgggccatc gtcaccggat
      361 cgcttagcag caccaggcga cgatcgcgat tcagcttctc tggcgtctcg ttaagcgcct
      421 gttcaacgtg gcggtcacgg gcgatttcca gcaccttact ggcgcggtga cgcgctgtcg
      481 ccattgcggt tgccagagcg ttaaatgacg ggttaaacac tgcgtgcata aagccatcgt
      541 caagggagcg ttggcgattc atctcaagga accgatcggt atcaaccagc acctgcggcg
      601 gtgaatactc ttccgggatc aggaacagtt tccagagttt ggtacgcaga ccaacggtgg
      661 cacggctgga aatcaccgaa acaaacggtg acaggatcaa cgagaagaca atcggtgcca
      721 gccagaacag gaaacgcaga tccagccacg ccatcccaac agcccacact aaccccaaca
      781 gcagctgtga gccgtggcgt ttgaacgctt cgccccatga agtggagtca tcatcacgct
      841 gcggtgaatt ccacaccact tcccagccaa ggaacgcgct gacaacgaag accgtatgga
      901 acagcatgcg taccggagcc agcagcacgg aaaacagcac ttccagcagc aacgataatg
      961 taacgcgcca gaagccgccg tattctttcg ttcctttgca ccagataagc aaaatgctca
     1021 ataacttcgg caggaacaac agcaccatgg tcgaagcaaa aagtgcaatc gccagctcag
     1081 gacgccactg cggccacacc gggaacaact gccgtggttg caggaagtat tgcggttcgg
     1141 tcaacgcatg cactacctgc aatgcagtag agagcgcgag gaacataaac cacagcggag
     1201 cggagagata agacatcacg cccgtcagga acaccgcacg gtgaaccggg tgcataccct
     1261 taaccaggaa cagacggaag ttcatcaggt taccgtggca ccagcggcgg tcacgtttta
     1321 gctcatcaag caagttaggc ggcaattctt cataagaacc cgggagatcg taagcaatcc
     1381 agacccccca acctgcacgg cgcatcaacg ccgcttctac gaagtcatgt gacaggattg
     1441 aaccggcaaa ggaaccttcg cccggcagcg gagccagtgc gcagtgctcg ataaatggtt
     1501 tcacgcggat aatcgcgtta tgtccccagt agtgcgactc gccaagttgc cagaagtgca
     1561 aaccggctgt aaacagtggc ccatacacgc gggtcgcgaa ctgctgacag cgcgcataca
     1621 gcgtatccat accggacgct ttcggcgacg actgaatgat cccggcgttc gggttggctt
     1681 ccatcaagcg caccaggccg cataaacaat caccggtcat taccgagtca gcatccagca
     1741 ccaccatgta gctgtactgg ctgccccagc gacggcagaa gtcatcgata ttaccgcttt
     1801 tacgcttcac gcggcgacgg cggcggcgat agaaaatctg accttcgcca ccgacttcag
     1861 cgataagctc catccaggct ttttgctctg cgacgcagat atccgggtta taactgtcac
     1921 tcagaatgta gacatcaaag tgtttggcat tcccggtggc ttttactgat tcccacgttg
     1981 cacgcaagcc agcaaaaaca cggttcacgt cttcgttaca gataggcatg atcaacgccg
     2041 tgcgatgctc cgggtttaat ggttcatcgc caactgttga cgcagatata ctgtatttat
     2101 cgcgaccaat aagcagttgc aggaagccca tcaacgccgt ccagaatccg gcggacaccc
     2161 aacagaacag taccgcaaaa aggatcagga taccggtttg cagcatataa ggcagaagct
     2221 gcataaagga aacccacaaa tcctgaccaa ccatatccat aggattaatc agcgcccaac
     2281 cctgataagg aagaatggtc ttcatatacc aggtcgcgac aacagtttgc gcgagcgtca
     2341 ggatcaacag aatgtaacgg cggatggtac caacggtacg ccacttttgc tcgctctcct
     2401 gctcttcttt ggtcaaacga gccagatagc gcggcgtgac atcgcgtcca cgcaggcgat
     2461 cccagaaacg gcctaccggg ttggtacgcc acgggtcggg aaacatcgag gagcgttttg
     2521 cttctggcat cgccttaagc tgatcgcgcc cttcgtcgtc tttaattaac tgtccatcag
     2581 caagtgaatc tggccaggct tgttccagac gcgcctttac cgagccttgc ggggaatcat
     2641 cctcccgcgc ccaggtgcgg tgttcggcat ccagcgcctg atgaacggcg cggatatcag
     2701 tcttcggcaa tgctgctttc tcgcttgcgg cgatgggcat tgcgtcaatg tactcagttg
     2761 tcttattcat tggcaggtaa ctggtagctc caggtttcac tcaacgtctg atcggcattc
     2821 accagcgcag cacgcatttc agtggttttc ttggcatctt tcactttcac acgcatcacc
     2881 agacgccagc ctttggtaac cgggttatag cgcaccgtgc tttcaactat ctcaccatta
     2941 tcaccaatgc tggtttgcgc tgtgaccggg gtatcctctg gcagtttttt catctctgcg
     3001 ccggtaaaat cgaccacaaa ggcgatagta ccgtcaggct ggcgaatcag gttcgactgc
     3061 ttcacatccc ccgttgaacg acgcgtttgt tgcacccatg cgttatctgg cgcatgcagt
     3121 ttgtcttcat cacggctgaa ggtgatggtg tatttaaagt tcatctcttt acccggctcc
     3181 ggcagctgat ccggcgtcca gtaagcgacg atgttatcgt tggtttcatc gttggttgga
     3241 atttccacca gctcaacgct gcctttgccc cactcccctt tcggagtcac ccatgcgctt
     3301 ggacggagat cgtaacgatc atcgagatct tcaaagcggg agaaatcacg acctcgctgc
     3361 aacagaccaa agcctcgcgg gttttccatg gagaagctgc tgaccgctaa atgtttcggg
     3421 ttattcaacg gacgccagat ccattcgcca ttaccagcat ggatagacag accgttagag
     3481 tcgtgcaact ccggacgata gttatttgca ggcgacggtt ggttcggccc aaacaggaac
     3541 atactggtta acggtgcaac ccccagtttg ccgactttat cgcgcagata gattttcgac
     3601 tgcacatcca caaccgtgtc acgccctggc ataaccacga atttgtaagc acctgtcgca
     3661 cgcggcgagt caagcaatgc ataaatggtt aaacgtttat cagtcggttt tggacgctcg
     3721 atccagaact ctttgaagcg tggaaattct tcacccgatg gcaaagcggt atcaattgct
     3781 aggccgcggg cagaaaggcc ataaacctga cctgcaccaa tcacgcggaa atagctggcc
     3841 ccgagcatgc tgacgatttc atcgttttta tctttgctgt tgatcgggta aagcacttta
     3901 aaaccggcaa aaccaaggtc ttttaccgtg tctttgtcat gctgaacatc gccgaaagtg
     3961 aaataatccg ggctgtattt gattcgtttg actgcggtgg cagtcacttc atttattttg
     4021 accggggtat cgaagtacat accctgatgg tagaactcga gtttgaatgg ggtcttcaga
     4081 ttgttccagt acgctttgtc atgattaaac tggatctgct gatagtccgc gtatttcatg
     4141 tcgcggaaaa cggagggcaa gttgctttta ggcgcctcat agcctttccc ggctaaggat
     4201 tgagcttgct ttgcgacatc atcaatactg aaagcccagc ttgaagatgt atacagggtt
     4261 aacattactg cagcactcaa ccaacgcatt ttcatcattt gtagtttatg tttcataatt
     4321 agtaagcact tccccctttg tgtgcttata tcgatccgat ctattttaat ggataaccag
     4381 ccattccgac aactgaatcc cggtatcgtt cagcacgctg gctcatggat taagcacctg
     4441 attgcaaccg ttttcacttt ccgtgcttct cctaaagaca gaagcatcga cttcgtgtag
     4501 ggtttgccag caaatttatt tatcaaccgt cttacagacg cttccattct ggttagtggt
     4561 agcgatgatg ctatgcataa aaaccttgaa gcctgcgcct ggcgcggtca ggtcggtgtc
     4621 gctatggcaa caagcctaaa ggcataaccc gacagggata agacgaaaaa tccgagatta
     4681 gttaaccata tatgaatcca gtacccgcgc aacgtgaata tttcctcgac tccatccgcg
     4741 cctggctgat gttgttaggg atcccttttc atatttcttt aatctattcg agccatacat
     4801 ggcatgtgaa tagcgccgaa ccgtcattgt ggctgaccct ttttaatgac ttcatccact
     4861 cgttccgcat gcaggtattt ttcgttatat ccggatactt ttcctacatg ctttttttac
     4921 gctatccctt gaaaaaatgg tggaaagtac gtgtcgaacg tgtaggtatc ccgatgttaa
     4981 cagccatccc cctactgaca ttaccgcaat ttattatgct gcaatatgtc aaagggaaag
     5041 cggaaagttg gcctgggcta tcattgtatg acaaatataa tacgttggcc tgggaattaa
     5101 tatcacacct gtggttttta ctggtgttag tagtcatgac gacgctgtgt gtatggatat
     5161 ttaagcgcat cagaaataat ttagaaaatt ctgataaaac gaataaaaaa ttctcgatgg
     5221 taaaactatc ggtgattttt ttatgcctcg gcatcggcta tgcggtaata agaagaacga
     5281 tttttattgt gtatccaccc attctgagta atggcatgtt caattttatt gtcatgcaaa
     5341 cgctatttta tttaccgttc tttatcctcg gcgcactggc tttcattttc cctcatctta
     5401 aagccttgtt taccacgccg tctcgtggct gtaccctcgc tgcagcattg gcgtttgtcg
     5461 cttatttact caaccagcgc tatggcagtg gcgatgcctg gatgtacgaa acagagtcgg
     5521 tgatcaccat ggtcctcggc ctgtggatgg tgaatgtggt cttctccttt ggccaccgtt
     5581 tgcttaactt ccagtcagcg cgggtgactt attttgttaa cgcatcgctg tttatctatc
     5641 tggttcacca cccgttaacg ctgtttttcg gcgcatacat tacaccgcac atcacctcca
     5701 actggcttgg ttttctctgt ggcctgatat tcgtagtagg gattgcgata attctgtatg
     5761 aaattcattt acgcatcccg ttactgaagt ttttgttctc tggtaaaccg gttgttaagc
     5821 gtgagaacga taaagcacca gcccgttaag ccacatttac aataaccatt ccacgggcaa
     5881 tatcgacgcc agtctgacca taacccgctt ccagaaactg gtggcggggt cttttttgag
     5941 aataacctct ttctttgcat gacgatcaac ccagttgatc cgtccccacc tgtccagacg
     6001 gagctgccag gccgcatcgt actggctctg aataaagcgt ttatcaatta actgtgccag
     6061 cgtttcgctc tctatcacga agcccatttc agtattgagc aatgttgaac gcggatcgaa
     6121 attgaaagag ccgataaaca ccgttttacc atcgatgcta aaagttttag catgcaggct
     6181 ggcaccggaa ttaccggtta tgccgcgatc gtgtaacgta ctgctttgtt cacgcgtcgg
     6241 cttgagttca tataattcca cgccatagcg gagcaatttt ttgcgccagc gcgcgtatcc
     6301 ggcatggacg acagcaacat cgttagcggc aagagaattg gttaggatcg caatctttac
     6361 cccttttctc accatccgta agagttgcgc cacacctgcg cgtgtcggta caaaatagga
     6421 agagataata tcgatgcgtt cactgggtga gcccatgata tcgaacaggc gctgcggtaa
     6481 cagtgaatgc cgttttgcct tgccctcccc tttcgccgga tcatcactta ataaacgtgt
     6541 cttcgcccag ataagcggca acgttccatc aatcagatga tttataaatg gactggattc
     6601 cattttgcgt aaataacgat gcgtcatggc atcgttatgc caggaggcgg gtaactcgat
     6661 gcgatccgcc atttcaccct ccgggacatc cagcacctgc tgtaagggtg aaaccgattt
     6721 gcaataccag tagcgggcga aatcatcggc aacgtcctct accacggggc ctattgccat
     6781 gacatctaaa tccgaaaaaa gtcgctcttc ccctgcacca aaataggcat caccaatatt
     6841 tcgtcctccc actagggtaa ccacgccatc gacagtgaaa cttttattgt gcatccggcg
     6901 attgagacgg gaaaagtcgg tgatatatcc aagcggacgt agcaagcgaa acgagaaagg
     6961 attaaaaagc cggacttcaa tgcgtggatg actgtcaagc aagcgtaaaa tgtcgtcaag
     7021 tccgggcgta ttgttgtcgt ccagcaacaa acggacgcga acgccacgct ttgctgcggc
     7081 taacagggcg gaaaacagta accgccccga catatcgtcc taccagatgt aatactgaac
     7141 atccagcgta tgctctgcca tttcggccag acgataccgg gcggcaaacg catccagact
     7201 tttctccaga gggaaaagac cacactgacc gggatgttgc gaacacagtg gcagcaccgc
     7261 gctcgccagc cggggcaaat cattcatctc cttgttgggt aaggagtctt tcgtagaggt
     7321 gggcgttttc ttcatcataa cagacaaagt atacctgttc gggtaaagcg tgacgggtaa
     7381 taaattctga aacggtttta actgcgattt cagccgctgc cgcacgaggg taaccataaa
     7441 ccccagtact gattgcagga aaagccactg acgtatagct gtttgccgcc accagtcgta
     7501 ggctattcag ataggcatcc tgcaaaagct ggtcttcgtt ttgttcacca ccacgccaga
     7561 ctggcccgac ggtgtgtact acggctttag cgggaagatc gcctgcaagc gtaataacgg
     7621 catgccccgt agggcaatcg ccctgctgtt gcctgacttt taaacaggca tccagcaggg
     7681 ccggacccgc tgcgcgatga atggccccat cgacgccgcc gcctcccatt aatgacggat
     7741 tagccgcatt cacaatcaca tcaacggcca gtttggtaat atcaccctgc acaacatgaa
     7801 tacgcgtttt cataacgcct ccttacacct tcttgtttct taagtgtaca gcaggctttt
     7861 tggttaaatc gcatttttct gctcagcaac gtgagttgtt cagaaactta gttatattcg
     7921 atgttcatta cagccagctt tttctgttta tcaagatact gcacttttac tgaggctatt
     7981 tgcgcgtttt tgacattgcc gctcattaac gctttgctgg agtaggtttt gcccggaata
     8041 gaaccattct atgggcagtt catttctacg ggtgatgact gggtgctgac gtcacatgcc
     8101 ggttccacaa tttggccata aaaatggatc acgccccctt gcgcggtgct tcctgcaatg
     8161 gcaataaaag gaaaaaacaa actgccgagc ataagagagt ctaaaaaagg tctgaacatt
     8221 tattctattt cctcaatgat tagtcatcct tgagggttgt gttatccata ctttcaggtt
     8281 ggctgattcg cagcagacca ttctctccag attcatctta tgctcgatat ttcaacaaat
     8341 taagactttt ctgaagaggg cgtccattgt tgtgataaat gaagtgcctg tccatcagaa
     8401 acagtaacaa ctattttcac ccgatcttcc ggggaaatat ttaaactcaa cttcgtcaaa
     8461 gcaatgggtt gattagcagg caatgagagg gtcttttctt gcttcgtctg actttgccct
     8521 gaactgcctt cgcgcaggga caatatttgt actctgcaca gacaaaattg agtaagagta
     8581 acttcaggaa taatggtgta catatcccct tgctgggtcg tattaaaggt tatctggctg
     8641 gaaagtgccg caaggagtaa taacgcattc atattcttct cccgaaaaaa aacagggctt
     8701 gcgccctgtt tctgtaatac aaatgatgta ttaatactga tgagcggtcg cgttgttacc
     8761 aaagccaacc tgagtcacgt tgacggagga gttcgatgca gtctggtcaa ctgtagcacc
     8821 gttgccacca ccgaactgtt taacagtcat ttcagaattt ttgccgttcc actgatcaag
     8881 agtggcgctg ttaccgaagc cacgttgggt cagatcgatt gagctgtcat ctgagccctg
     8941 accaacatct gcaccattac caccgccatg ctgggtaata gtcaagtcag agttacgggc
     9001 atcagtttgc agagcaagtg cagagttacc gccaccgtac tggtaaatgt tcagctctga
     9061 atttgggccg ctattatta
//
LOCUS       DAETPZ010000128         9041 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.129, whole genome shotgun sequence.
ACCESSION   DAETPZ010000128 DAETPZ010000000
VERSION     DAETPZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.129"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="K0W59_003635"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="K0W59_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HBI3024504.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1860)
                     /gene="phoA"
                     /locus_tag="K0W59_003636"
                     /pseudo
     CDS             complement(446..1860)
                     /gene="phoA"
                     /locus_tag="K0W59_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
     gene            complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="K0W59_003637"
     CDS             complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="K0W59_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HBI3024505.1"
                     /translation="MKNLITELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(2403..2606)
                     /locus_tag="K0W59_003638"
     CDS             complement(2403..2606)
                     /locus_tag="K0W59_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024506.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2684..3778
                     /gene="ddlA"
                     /locus_tag="K0W59_003639"
     CDS             2684..3778
                     /gene="ddlA"
                     /locus_tag="K0W59_003639"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBI3024507.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASATCMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGELVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITHLIELALERHAADNALKTTM"
     gene            complement(3802..4014)
                     /locus_tag="K0W59_003640"
     CDS             complement(3802..4014)
                     /locus_tag="K0W59_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HBI3024508.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4274..4582
                     /locus_tag="K0W59_003641"
     CDS             4274..4582
                     /locus_tag="K0W59_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HBI3024509.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="K0W59_003642"
     CDS             complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="K0W59_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HBI3024510.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGSYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="K0W59_003643"
     CDS             complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="K0W59_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HBI3024511.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7320..8477
                     /gene="ampH"
                     /locus_tag="K0W59_003644"
     CDS             7320..8477
                     /gene="ampH"
                     /locus_tag="K0W59_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HBI3024512.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8478..8981)
                     /locus_tag="K0W59_003645"
     CDS             complement(8478..8981)
                     /locus_tag="K0W59_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3024513.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
BASE COUNT         2240 a         2314 c         2149 g         2338 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagattgt tgcgttgctt gattattaca ttcgcgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cggttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca aggattcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca ccggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt ttttattcag cgcatagtga gtgtattgcc cggtaagcgg
     1561 taaggcatcg atacctttaa aaaagccgcc cgcaccttcg gcataattac gtgcggcagt
     1621 aatttccgaa tcccccatcc catcgccaat cagcaaaata atattttttg caggtttatc
     1681 gctaagagaa tcacgcagag cggcggtctg atcacccgtt aagcggcgag caccgccggg
     1741 tgcagtaata tcgccctgag cagcccggtt ttccagaaca ggcatttctg gtgtccgggc
     1801 ttttgtcaca ggggtaaaca gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac
     1861 tttattttct ccatgtacaa atacattaaa aaataaaaac aaagcgacta taagtctcgg
     1921 ccgtgacaac tttatgacag ctattgaaaa gattaacttt ttactgacga ggatgcttca
     1981 ataacttctt tacgtaatcg cgcagcagct ccgtatcgtc gtcaggaatg ctggcatcgg
     2041 gctttacctc gtacagcgcc ccctctacct gatcaatcaa ccgctgttgg tcattttgcg
     2101 ccatattgcg aagcattgca gtgacgataa tctccaaagc ttctacctgc gcacacagtt
     2161 ctttcgactc ttcttctttt tgggcaagct taaataacaa ctcagtaatg agatttttca
     2221 tgtctgtatt tccttatcca aagtatggag aagttaacat tatgtttcac aacttcatag
     2281 tgtctggaag tattattttt atgacaaagg aaatactttg cgcaaatatt ttttgtttga
     2341 ttaccagcga aacgtttctc ttatttaaat aattacccgc acaaaatata ggcttttaat
     2401 tatcaacctt tattataaat aaaagctatg aaatatattc tcttcgttaa cttataatta
     2461 agagaaaaaa actcaccgca ttgccattta agccaggaat aaattagctt cccgttcatc
     2521 atttttgtga tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac
     2581 gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg
     2641 tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt
     2701 aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa
     2761 cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca
     2821 agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca
     2881 tattgcgttg cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca
     2941 gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccaattgt
     3001 ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc
     3061 gtttgtaggt tctgatgttc tggcttcagc aacctgtatg gataaagatg tcaccaaacg
     3121 tctgctacgc gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg
     3181 tcacaacatc agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc
     3241 ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat
     3301 tgccgtcgat ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg
     3361 tcgtgagatc gaatgcgcag ttctgggcaa cgacaatccg caagccagca cctgtggcga
     3421 gctcgtactc accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc
     3481 gaaagtggta gttccggcag ccattgcacc agagatcaac gataagatcc gggcgattgc
     3541 cgttcaggct tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac
     3601 cccagagaac gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag
     3661 tatgtatccg aagctgtggc aagccagcgg tctgggttac accgatctga tcacacattt
     3721 gattgagctg gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata
     3781 ttttaacgcc cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat
     3841 aatcaacccc gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg
     3901 ccaacctttt gcttcaaatc ccagtacccc caccacgcca ttaagaatga atataaggcc
     3961 aatggcgaac gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc
     4021 tccgttaacg aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa
     4081 cattttcgta attgttaata aatttaactt aaggttgcca ttttgtgact cacaacgaag
     4141 attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta
     4201 aatgacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact
     4261 tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg
     4321 caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg
     4381 ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat
     4441 gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt
     4501 cgggctgatc ccgtttttag caggctgcct catttttgca gtggtggcgc tatggctgca
     4561 ctggcgacat cgccgccagt aaaatttacg ttgccagatg cggcctgaaa cgccttatct
     4621 ggcctgacat tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc
     4681 ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca
     4741 ggcaacattt cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc
     4801 ttgtacagcg ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt
     4861 tcgctgtcgc tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg
     4921 ggttctttac tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc
     4981 ttactgactg cgttctgaaa cgcggcatta cggctggcgt accagccagc gttaaaatca
     5041 gcaaagcggt atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagaacca
     5101 aaccacaacc cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac
     5161 ggataccctt tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg
     5221 cgcaccggat taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg
     5281 tcaaaaatcg cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt
     5341 ttgccgtttg gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc
     5401 cgttcagcac gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga
     5461 tctgcctgat aatttgactc ctgctgcgcc accgccagca ccgagcagac gttttccagc
     5521 gtcggtgcca gcccctgact ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg
     5581 tctttcacgc tcgcaggcat cttctgccgc acgacgctcg ctacatctat cgctttttcg
     5641 ccctttttca acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac
     5701 aatgacagcg aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta
     5761 tgggttactt cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg
     5821 tagatagaca tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg
     5881 cgaacctgac caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca
     5941 acaattgacg gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg
     6001 cgggcgatgt tgaaatacat atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg
     6061 ctaaacagct cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc
     6121 agctctttac ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg
     6181 atccctacca ctgccagcaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc
     6241 agaccatacg gaatgtgccc gataatcggc agctccggca catgcgcgga gagcgttacc
     6301 agcaccggca ggaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc
     6361 tccagcgttg aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata
     6421 tgacgcagtt gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac
     6481 acgtagtgac tgacaaagaa gttgttcagt acactgatca ccaccgcgat cagcgcaatc
     6541 cccagaaaga cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt
     6601 agcgcggttt gaatcagatc atagaacggc gcataccagg cgttgacggc gaccccgact
     6661 tccaccaaaa accaagtgac gaagataatc agcgcagtac cgagtattga ccagtattgc
     6721 caacggtgcg ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag
     6781 taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc
     6841 ccggaagcgc cggtgatacg tgccacccag tcacccccac cggcttgcca gaagataacg
     6901 gcaatcaatg cccaaacaaa ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac
     6961 ttaaacatat gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc
     7021 atgaccgcta caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct
     7081 tagttcgcca gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt
     7141 acataagcgg attaacacgc gcaggatcac aaatgcaggc agtggtgagc aggaaaaaga
     7201 tcgtgtttac gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat
     7261 ccatcaatga gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt
     7321 tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg
     7381 cggcgcagcc gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc
     7441 atatttttta cggcagcggt gccacgggga tggcgctggt tgttatcgac ggtaaccagc
     7501 gcgtctttcg tagttatggc gaaacgcgac ctggtaataa cgttcgcccg cagctggatt
     7561 cggtcgtgcg tattgcttcc ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc
     7621 tcgaccaggg gaccgtaaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac
     7681 gcgtgccgac ctacaatggg acgcccatta ccctagtgaa tctggcaacc cataccagcg
     7741 ccctgccccg tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc
     7801 gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg
     7861 cggcgtactc taaccttgcg ttcgacctac tggcagatgc actggcgaat gcgtccggca
     7921 agccttatac ccagttgttt gaagagcaga ttacccatcc attggggatg aaagacacca
     7981 cctacacccc ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc
     8041 cgtgcaataa cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata
     8101 tgatgcgctg gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag
     8161 atcgcatgca gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg
     8221 tccccggcaa agcggatgcg cttggtttag gctgggtata tatggccccg aaagagggtc
     8281 gtccgaggat tattcagaag acaggcggtg gtggcggttt cattacctat atggcgatga
     8341 tcccgcagaa gaatattggc gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta
     8401 aaaatatgag cgatggcatc aatgatctgg tgaccgagct aagcgggaat aaaccgttgg
     8461 ttatccccgc atcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct
     8521 ttattcattt cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa
     8581 cgcgatatgc gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct
     8641 tcattccagt caatcattga taataatgtt gcgcgggttt gtttggctgg tagatgatat
     8701 cccgtacaat ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca
     8761 gccagcccca taatataagg agcctgggta ataccgataa gtacgttttc ttctctacgc
     8821 agagagataa ctccttctaa aataacgaaa gtatcttcgt tattgacatc actggaaaat
     8881 ataacttgct tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg
     8941 agcttaccaa attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta
     9001 atgcccgtca aatccttgac gggcattatt tagattaaat t
//
LOCUS       DAETPZ010000129         9011 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.130, whole genome shotgun sequence.
ACCESSION   DAETPZ010000129 DAETPZ010000000
VERSION     DAETPZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.130"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(96..1472)
                     /gene="trkA"
                     /locus_tag="K0W59_003646"
     CDS             complement(96..1472)
                     /gene="trkA"
                     /locus_tag="K0W59_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HBI3024514.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1494..2783)
                     /gene="rsmB"
                     /locus_tag="K0W59_003647"
     CDS             complement(1494..2783)
                     /gene="rsmB"
                     /locus_tag="K0W59_003647"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBI3024515.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2829..3776)
                     /gene="fmt"
                     /locus_tag="K0W59_003648"
     CDS             complement(2829..3776)
                     /gene="fmt"
                     /locus_tag="K0W59_003648"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBI3024516.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3791..4300)
                     /gene="def"
                     /locus_tag="K0W59_003649"
     CDS             complement(3791..4300)
                     /gene="def"
                     /locus_tag="K0W59_003649"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBI3024517.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4448..5572
                     /gene="dprA"
                     /locus_tag="K0W59_003650"
     CDS             4448..5572
                     /gene="dprA"
                     /locus_tag="K0W59_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBI3024518.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNIIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5544..6017
                     /locus_tag="K0W59_003651"
     CDS             5544..6017
                     /locus_tag="K0W59_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HBI3024519.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6046..6588
                     /locus_tag="K0W59_003652"
     CDS             6046..6588
                     /locus_tag="K0W59_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HBI3024520.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6593..7165
                     /gene="tsaC"
                     /locus_tag="K0W59_003653"
     CDS             6593..7165
                     /gene="tsaC"
                     /locus_tag="K0W59_003653"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HBI3024521.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7170..7988
                     /gene="aroE"
                     /locus_tag="K0W59_003654"
     CDS             7170..7988
                     /gene="aroE"
                     /locus_tag="K0W59_003654"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBI3024522.1"
                     /translation="METYAVFGNPIAHSKSPLIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7985..8242
                     /locus_tag="K0W59_003655"
     CDS             7985..8242
                     /locus_tag="K0W59_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HBI3024523.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8218..8772)
                     /locus_tag="K0W59_003656"
     CDS             complement(8218..8772)
                     /locus_tag="K0W59_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBI3024524.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2256 a         2189 c         2248 g         2318 t
ORIGIN      
        1 taacaaatgt taagccattt tcctgccagg aatgaattta attaatcctt gataatcaaa
       61 gggaaataat aaggcgtcat tagacgcctt attaattaca agaagaaagg gcttggctgg
      121 aagagtcttt cgacgtcggt aataaacttt ttatctgtga ggaacataat tacgtgatcg
      181 ccttgctcaa tgcgcagatt gtcattggca atcatcacgt cgtttccacg taccaccgct
      241 ccaataatcg ttcctggcgg tagcttgatt tcgtcaatga ctctgccgac aacgcgtgag
      301 gtgctttcat caccgtgagc aacggcttca atagcttctg ctacgccgcg gcgcaatgag
      361 gaaacaccaa caatatctgc ttttcgcaca tggctaagca acgcagaaat agttgcttgt
      421 tgtggtgaaa ttgcaatatc gatcacgctc ccctgaacca gatccacata agcgcgacgc
      481 tggatcaata ccatcacctt tttcgcaccc atacgtttgg caagcatggc ggacatgata
      541 ttggcctcgt catcgttggt gacggcaata aacagatcaa cttgatcgat atgttcttcg
      601 gccagcagtt cttgatccga cgcatcacca aaaaagacga tcgtattctg caacttttcc
      661 gccagttcgg cagcgcgctg ctgattacgt tcgatgagtt taacgctgta atctttttcc
      721 agacgacgcg ccagccctgc accgatatta ccgccaccaa ccagcatgat ccgtttatac
      781 ggtttttcca gtcgctgtaa ttcactcatc accgcgcgga tatgctgtga agcggcaata
      841 aagaacactt catcaccagc ttcaacaatg gtcgaacctt gtggacgaat ggggcgatcg
      901 tggcggaaaa ttgctgccac acgagtatcg atatgtggca tatgttcgcg catggtcgaa
      961 agtgcattac caatcagcgg gccgccataa taggctttaa ctacagcgag gctgacttta
     1021 ccctcagcga agttcaccac ctgcaatgcg cctggatact caatcagtcg gtaaatatta
     1081 tcgataacca actgctctgg tgcgatcaga tgatcaatcg gtacagcatc tgaatgaaat
     1141 agcttatcgg catcgcgtac gtagtctggt gagcggatgc gagcgatgcg attaggggtg
     1201 ttgaaaagtg agtaggctac ctggcaggca accatattgg tttcatctga actggttaca
     1261 gcaaccagca tatcggcgtc gtcggcacct gcctcccgca atacgcgtgg atgagagcca
     1321 tgcccctgca cgactcgcag gtcaaattta tcctgcaagg tccgcagacg ctcaccgttg
     1381 gtatcgacaa cagtaatatc gttgttctcg ccaaccaggt tttccgccag tgtgccgcca
     1441 acctggccgg cacccagaat gataattttc atcagtcgcg acccgttatc tcatcacttt
     1501 ttgattagct tagcgtaaaa gaagccgtcg ccctcttcgg caccaggctg attttgttta
     1561 cccggttgct ctggtgttcc tgtttcgcaa agttcggcat cagcggtacg ttgcaaaaag
     1621 gctttaatct gcaggctatt ctcttccggt aacaccgaac aggtggcata gaccagggtt
     1681 ccaccggatt ttaaatgcgg ccaaatggcg tcgagaattt cagactgcaa ttgcgcgagt
     1741 tccgggatat cgcgatcgcg acgtaaccat ttaatatctg gatggcgacg aatcaccccc
     1801 gttgctgaac aaggcgcatc taataaaatg cgatcaaact gttgctcgcc acaccattgg
     1861 gaagggtaac ggccatcacc ttgtttcacg gttgccttca taccaaggcg ttttaaattg
     1921 tcgtaaacgc gagagaggcg ctgttcgtca atatcaaccg caacaacctg cgcttctggt
     1981 gccacctcaa ggatatgcgt tgttttaccg ccgggggcgg cacaaagatc caagatgtgt
     2041 tcaccgtttt gtggcgcaag ccaggtcatg caaccttgtg ctgatgcatc ctgaacggta
     2101 acccatccgt cttcaaaacc aggtagcgca tgaacaggtg caggtgtttc cagccgtaca
     2161 gcatcagggt aatccgcatg cgggaaacct ttcattcctg cttcatccag caatgcaagc
     2221 cagctgtcgc gggaatgatg cgtacgatta atacgcagcc acattggcgg acgctggtta
     2281 ttggcttcgg cgatggattg ccactgctct ggatacgctt tttgcagacg cttcagcaac
     2341 caggaaggat gcagataacg tgcatcacgg gtattaaact cggctaataa ctcttcttgc
     2401 cgacgctgga actggcgtaa tacaccgtta atcaaccctt taagttgcgg acgcttaatt
     2461 gcgatagcgc cttcaaccgt ttcagccagc gcagcatgag gtggaatgcg ggtataaagc
     2521 agttgataca aaccaaccat aatcaggtaa tgcacagtgc gctgtttgcc ggtcatcgga
     2581 cgggccatta acttattaat cagccagtct aactgcgaaa gcgtacgcag tacgccaaag
     2641 cacaactctt gaagaagtgc tttgtcttta tcggaaactt tttgctggag cggtggcaga
     2701 atgttgctta atgattgccc ttgctcgacg acttgttcaa cggcctgggc cgccatgcta
     2761 cgtaaattac gttgtttttt cataagtata aaaatgctcg gcaagaccgg gcttagaaga
     2821 gtggactatc agaccagacg gttgcccgga acaaaccatt cccgacgaga gttcaggagg
     2881 tcttgcgcgc tcatcgcttt cttacccgca ggttgtaacg agagcaggtt caggatgcca
     2941 tcaccagtcg caacctgaat gccttgtttg ttggcttcaa ggatcgttcc tggtgcagcg
     3001 ttggttgccg tatcaatgac cgacgctttc cagactttaa ccggctgtcc ttcaatttcc
     3061 agccagctca ttggccatgg gttgaaagcg cgaatgcagc gttcaagctg tgctgccgaa
     3121 agtgaccagt caatacgcgc ttcttcttta ctcaacttct cggcgtaagt gacaagagtt
     3181 tcgtcctgaa cttctggttt cgccgtgccg tctgccaatt gtttcaacgt ggtgataagc
     3241 ccttgtgggc caagctctgc cagcttgtcg tacagcgtac cactggtatc ttctgcagta
     3301 atcgggcagg agagcttata gagcatgtca ccggtgtcta aaccaacatc catttgcata
     3361 atggttacac cagtttctgc atcacccgcc catagtgagc gttggattgg cgcagcaccg
     3421 cgccagcgtg gcagcagtga accatgaacg ttgatacagc caagacgcgg catctccagc
     3481 acagctttag gcagaattaa accataggcg acgacgacca taacatcagc ctgcaaatcg
     3541 gcgaccagtt gctggttttc ttgtggacgc agggaaacag gttgaaaaac gggcagacct
     3601 tcttcctcag ccagaacttt aatcgggctg ggcatcagtt ttttaccgcg tcctgccggt
     3661 cggtctggct gggtgaacac gccaacgacg ttatgaccag aagacaacag cgcgtcgaga
     3721 tgacgcgctg caaagtcagg tgtacccgca aaaataatac gtagtgattc tgacacgtta
     3781 gttcttatcc ttaagcccgg gctttcagac gatccagttt ttcaactttc tgacgaattc
     3841 gttgttgttt cagcggtgac agataatcca taaacagttt gccaaccagg tgatccatct
     3901 catgctgaat acagatagct aacagaccct ctgcttccag ttcaaatggt ttaccgtcgc
     3961 ggtcaagggc gcgaatttta actttctctg cgcgcggcac taaagcacgt tgttcaggga
     4021 tcgacaggca accttcttca atgcctgttt cgccgctttt ttctaaaagc tccggattga
     4081 ttaacaccag tcgttcgtca cggttttccg aaacatcaat aacaatgata cgttgatgga
     4141 tatcaacctg ggttgccgct aggccaatac cttcttctgc gtacatcgtc tcgaacatat
     4201 catcgacgat acgctgaatt tctgcattca cttcttctac cggtttagca actttgcgaa
     4261 gccgctcgtc cggaatatgt aacacttgca aaactgacat aaatctccag agatgtgttc
     4321 aggagttaga aagattattt cttctattct agacaaatcc ccctctgatt gacagcatca
     4381 cttgattgac agcatcactg accaatcgca aagattgcta aggcttgctt atgacaggga
     4441 gataaggatg gtcgatacag aaatttggct gcgtttaatg agcatcagca gcttgtacgg
     4501 cgatgatatg gtccgtatcg ctcactggct ggcaaaacag tcgcatattg atgcggttgt
     4561 attgcagcaa acagggctta cattgcggca ggcacaacgc tttctttcat ttccacgaaa
     4621 gagtatcgaa agctcacttt gttggttgga gcaacccaac catcatttaa tccctgcgga
     4681 cagcgaattt tatcctcctc aacttcaggc gacgacagat taccccggcg cactgtttgt
     4741 tgaaggagaa ctgcacgcgc tgcattcatt tcagcttgcc gtagtgggga gtcgggcgca
     4801 ttcatggtat ggcgagcgat gggggcgatt attttgcgaa actctggcga cgtgtggagt
     4861 gacaattacg agtggactgg cgcgtggaat cgatggtgtg gtgcataaag cggccttaca
     4921 ggtaaatggc gtcagcattg ctgtattggg gaatggactt aatatcattc atccgcgtcg
     4981 ccatgcccga ctggctgcca gtctgtttga acagggtggc gctctcgtct cggaatttcc
     5041 cctcgatgtt ccaccccttg cttacaattt cccacgaaga aatcgcatta tcagtggtct
     5101 aagtaaaggt gtactggtgg tggaagcggc tttgcgcagt ggttcgctgg tgacagcacg
     5161 ttgtgcgctt gagcaggggc gtgaagtttt tgccttgcca ggtccaatag ggaatccggg
     5221 aagcgaaggg cctcactggt taataaaaca aggtgcgatt cttgtgacgg aaccggaaga
     5281 aattctggaa aacttgcaat ttggattgca ctggttgcca gacgcccctg aaaattcatt
     5341 ttattcacca gatcagcaag acgtggcatt gccatttcct gagctcctgg ctaacgtagg
     5401 agatgaggta acacctgttg acgtcgtcgc tgaacgtgcc ggccaacctg tgccagaggt
     5461 agttactcaa ctactcgaac tggagttagc aggatggatc gcagctgtac ccggcggcta
     5521 tgtccgattg aggagggcat gccatgttcg acgtactaat gtatttgttt gaaacctata
     5581 ttcacactga agctgagttg cgtgtggatc aagacaaact tgaacaggat cttaccgacg
     5641 caggttttga gcgagaagat atctacaatg ccctgctatg gctggaaaaa cttgctgatt
     5701 atcaggaagg gttggcagaa ccgatgcaac tggcctcgga tcctctctcc atgcgtattt
     5761 atacaccgga agagtgtgaa cgactggatg ccagctgccg tgggtttctg cttttccttg
     5821 agcagattca ggtgctcaac cttgaaactc gtgaaatggt gatagagcga gtgctcgcgc
     5881 tggataacgc agagttcgaa ctggatgatc tgaaatgggt gatcctgatg gtgttgttca
     5941 acatcccggg ctgcgaaaat gcgtaccagc aaatggaaga attactcttt gaagtgaatg
     6001 aaggtatgct gcattaatca tttctttaat tcagcataag ttgttatggc aaaatcagca
     6061 ctgttcacgg tgcgtaataa tgagtcctgc ccaaagtgcg gggctgaact ggttattcga
     6121 tccgggaaac acggtccgtt tcttggatgc tcacagtatc cggcgtgtga ctacgtccgt
     6181 cctctgaaat cttcagcgga tggacatatt gtcaaagttc tggaggggca ggtttgcccc
     6241 gtatgtggcg caaatctggt attacgccag ggacgctttg gtatgtttat tggttgcagt
     6301 aactaccctg aatgcgaaca taccgaactt atcgataaac cggacgaaac agcaattaca
     6361 tgcccccaat gtcggacggg ccatctggtc cagcgccgct cccgttatgg caaaacattt
     6421 cactcctgtg atcgctaccc ggagtgtcaa tttgccatta acttcaaacc aatagctgga
     6481 gaatgccctg agtgtcatta tccgctactc atcgaaaaga aaaccgcgca gggtgtaaaa
     6541 cacttttgtg ccagtaaaca atgtggaaag ccggtttcgg cggaataata acgtgaataa
     6601 taacctgcaa ggagacgcta tcgcagctgc gatagatgtc ctcaatgaaa aacgtgtcat
     6661 cgcctatcca acggaagccg ttttcggtgt cgggtgcgat cctgatagcg aaacagcagt
     6721 gatgagactg ttggagttaa aacagcgtcc ggttgataag gggctgattt taatcgcggc
     6781 aaattacgag cagcttaaac cctatattga tgacaccatg ttgactgacg tgcagcgtga
     6841 aaccattttt tcctgctggc caggtcctgt cacctttgtc tttcccgcgc ctgcgacaac
     6901 accgcgctgg ttgacgggcc gctttgattc gcttgctgta cgagtcaccg accatccgtt
     6961 ggtggttgct ttgtgccagg cttatggtaa accgctggtt tctaccagtg ccaacttgag
     7021 tggattgcca ccttgtcgaa cagtagacga agttcgcgca caatttggcg cggcgttccc
     7081 ggttgtgcct ggtgaaacgg gggggcgttt aaatccttca gaaatccgcg atgccctgac
     7141 gggtgaactg tttcgacagg ggtaacataa tggaaaccta tgctgttttt ggtaatccga
     7201 tagcccacag caaatcgcca ctcattcatc agcaatttgc tcagcaactg aatattgaac
     7261 atccctatgg gcgcgtgttg gcacccatca atgatttcat caacacactg aacgctttct
     7321 ttcgtgctgg tggtaaaggt gcgaatgtga cggtgccttt taaagaagag gcttttgcca
     7381 gagcggatga gcttactgaa cgggcagcgt tggctggtgc agttaatacc ctcaagcggt
     7441 tagaagatgg acgcctgctg ggtgacaata ccgatggtgt aggcttgtta agcgatctgg
     7501 aacgtctgtc ttttatccgc cctggtttac gtattctgct tatcggcgct ggtggagcat
     7561 ctcgcggcgt actactgcca cttctttccc tggactgtgc ggtgacaatc actaatcgga
     7621 cggtatcccg cgcggaagag ttggctaaat tgtttgcgca cactggcagt attcaggcgt
     7681 tgggtatgga cgaactggaa ggtcatgagt ttgatctcat tattaatgca acatccagtg
     7741 gcatcagtgg tgatattccg gcgatcccgt catcgctcat tcatccaggc atttattgct
     7801 atgacatgtt ctatcagaaa ggaaaaactc cttttctggc atggtgtgag cagcgaggct
     7861 caaagcgtaa tgctgatggt ttaggaatgc tggtggcaca ggcggctcat gcctttcttc
     7921 tctggcacgg tgttctgcct gacgtagaac cagttataaa gcaattgcag gaggaattgt
     7981 ccgcgtgaat caggccatcc agtttccgga cagggaagag tgggacgaga ataaaaaatg
     8041 tgtatgtttt cccgctctcg tgaatggtat gcaactgaca tgcgcgatct ctggcgagag
     8101 tctggcgtat cgctttactg gagatacgcc agaacagtgg ttagcgagtt ttcgtcagca
     8161 tcgctgggac ctggaagaag aagcggaaaa cttaattcag gaacaaagtg aagatgatca
     8221 aggctgggtc tggttaccct gatccagata ttcgtccttc catttcacgt aattattcgc
     8281 ggaatagcgt aacccagcct tctcttcatc acttaacggg cggatctgtt tgacggggct
     8341 accgagatac agatatccgc tctccagccg tttattttgt gggaccagac tacccgcacc
     8401 aatcatcaca tcatcttcta ctattgcgcc atcaagtaaa attgagccca tcccaaccaa
     8461 aactcgattg ccaatggtgc agccgtggag catcaccttg tgaccaacag tgacatcttc
     8521 gccaatggtt aatgggttgc catctgggtt gtacgaggat ttatgagtga catgcaacat
     8581 actgccatcc tggatattgg tgcgtgctcc gatctgtaca taatgtacat ctccacgaat
     8641 cacaacgagc ggccagatcc ccacatcatc agccagacga acgtcaccaa tcacgacact
     8701 gctatcgtcg atcattacgc gctgaccgat ttgtggaaaa agatcgcggt atgggcgtaa
     8761 aacatcagac atacttacct cagcaataaa tgatttacta atgactttgg gggcattatt
     8821 ggccttgtgc aagtctttta gtatgcaaaa aagcaccgtt ttgtgtgcga ttgcagcaaa
     8881 aagggtgaaa aaacaacaaa cagaaaaaaa gatcaaaaaa atacttgtgc aaaatattgg
     8941 gaaccctata atgcgcctcc gttgagacga caacgtgaaa cacttcacag gatggtcgga
     9001 acaacgaaga g
//
LOCUS       DAETPZ010000130         9007 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.131, whole genome shotgun sequence.
ACCESSION   DAETPZ010000130 DAETPZ010000000
VERSION     DAETPZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.131"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            111..2998
                     /locus_tag="K0W59_003657"
                     /pseudo
     CDS             111..2998
                     /locus_tag="K0W59_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(3108..3956)
                     /gene="alkA"
                     /locus_tag="K0W59_003658"
     CDS             complement(3108..3956)
                     /gene="alkA"
                     /locus_tag="K0W59_003658"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HBI3024525.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            4090..5442
                     /gene="yegD"
                     /locus_tag="K0W59_003659"
     CDS             4090..5442
                     /gene="yegD"
                     /locus_tag="K0W59_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBI3024526.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(5454..7394)
                     /gene="yegI"
                     /locus_tag="K0W59_003660"
                     /pseudo
     CDS             complement(5454..7394)
                     /gene="yegI"
                     /locus_tag="K0W59_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7391..8153)
                     /locus_tag="K0W59_003661"
                     /pseudo
     CDS             complement(7391..8153)
                     /locus_tag="K0W59_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
     gene            complement(8150..8809)
                     /locus_tag="K0W59_003662"
     CDS             complement(8150..8809)
                     /locus_tag="K0W59_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBI3024527.1"
                     /translation="MSEQITFATSDFASNPELRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2197 a         2389 c         2357 g         2064 t
ORIGIN      
        1 accagacccg gtgacatcac cccccggatc atggacgact gggggcagta atcatgccgg
       61 gtctggctgg ctaacacccc attataaggg gtacgaataa agtaacgggg ctggttgtcc
      121 tgctgatgcc cggagacgat cctctcaggg catttttgat atgggtactg tcagaatcca
      181 tcggcgctct ggcactggtg ccgctgggat tgttatttaa accacactat ctgctgcgcc
      241 atcgcaaccc acggttgctt tttgagtcgc tgctcacgtt agccatcaca ctgacgttaa
      301 gctggctttc gatgctgtat ctgccgtggc cttttacttt cattattgtg ctgttgatgt
      361 ggagcgccgt gcgcctgcca cgaacggaag cctttttgat cttccttacc acggtgatga
      421 tggtgtcgct gatgatggcc gcggatccct ccctgcttgc tacgccgcgt acgtacctga
      481 tgagccatat gccgtggcta ccgtttttgc tgatcctgct gcccgccaac atcatgacca
      541 tggtgatgta tgcctttcgt gcggaacgca aacacatttc cgaaagcgaa acccgctttc
      601 ggaacgcgat ggaatattcc gctatcggta tggcgttagt gggcaccgag ggacaatggc
      661 tgcaatccaa caaagcactc tgccagtttc tcggttacag tcaggaagag ctgtgcggac
      721 tcacctttca gcaactgacc tggccggagg atctcaataa agatctccaa caggttgaaa
      781 agctgataag cggtgaaata aacacctatt caatggaaaa acgctactac aaccgcaatg
      841 gcgatgttgt ctgggcgttg cttgccgtct cactggtgcg ccacacggat ggcacgccgc
      901 tctattttat cgctcagatt gaagacatta acgagctaaa acgcaccgaa caggtgaatc
      961 agcaactgat ggagcgcatc acgctggcta acgaagcggg cgggattggc atctgggagt
     1021 gggagctgaa gccgaatatt tttagctggg ataagcggat gttcgagctg tatgaaattc
     1081 ctccgcatat caaaccgaac tggcaggtgt ggtacgagtg cgtgctgccg gaagatcgcc
     1141 agcatgccga aaaagtgatt cgttgcaatc acgctcgccc tttaagctgg aatttcgcat
     1201 tgccgtgaaa gatggcatcc gccatatccg cgccctcgcc aaccgggtac tgaataaaga
     1261 aggcgaagtc gaacgcctgc tcggcattaa tatggatatg accgaggtta aacagcttaa
     1321 cgaggcattg tttcaggaaa aagagcgcct gcacattacg ctggattcca tcggtgaagc
     1381 cgtggtctgt attgatatgg cgatgaaaat tacctttatg aatccagcgg cggagaagat
     1441 gagcggctgg acgcaggaag aagcattagg tgttccgctc ctgacggtgt tgcatattac
     1501 ttttggcgac aacggaccat taatggagaa catttacagt gccgacacct cacgttccgc
     1561 gattgaacaa gatgtggtgt tgcactgccg gaacggcggc agttacgacg tgcattacag
     1621 tattacgccg ttaagtactc tggacggcag caatataggc tcggtactgg tgattcagga
     1681 cgtcaccgaa tcacgcaaaa tgctgcgcca gctgagctac agcgcctccc atgatgcact
     1741 gacgcatctc gccaatcgcg ccagttttga gaagcaactg cgtatcctgc tgcaaacggt
     1801 aaacagtaca catcagcgac atgccctggt gtttatcgat cttgatcgct ttaaagcggt
     1861 gaatgacagc gccgggcatg cggcgggtga cgctttactg cgcgaactgg cgtcgttgat
     1921 gctgagtatg ctgcgctcca gcgacgtgct ggcgcgactc ggtggtgatg aatttggtct
     1981 actgttgcca gactgtaata tcgaaagtgc gcgttttatc gctacacgca ttatcagtgc
     2041 cgtgaatgat tatcacttta tatgggaagg ccgtgtgcat cgggtaggtg ccagtgccgg
     2101 gattaccttg attgatgaca acaatcatca ggcggcagaa gtgatgtcgc aggctgatat
     2161 cgcctgttat gcctccaaaa atggtggacg gggccgggtg acggtttacg aaccacagca
     2221 agctgccgca catagcgaac gggcggcgat gtcgcttgat gaacagtggc ggatgattaa
     2281 agagaatcag ttgatgatga tcgcccacgg tgtcgcttcg ccgcggatcc cggaagcgcg
     2341 taatttgtgg ctgatttcac ttaagctctg gagttgcgaa ggcgagatta ttgatgaaca
     2401 aaaatttcgt cgtagcttca gcgatccggc acttagccat gctcttgacc gacgggtatt
     2461 ccacgaattt ttccagcagg ccgcaaaagc ggttgccagt aaaggcataa gcatcgccct
     2521 ccccctttcc gttgccggtt tgagtagcgc cacgctggtg aatgatctgc ttgagcagct
     2581 ggaaaatagc cctctaccac cacggttatt acatctgatt attccggctg aagcgatttt
     2641 agatcacgca gaaagcgtgc aaaaactgcg gctggcggga tgtcggatag tgctcagcca
     2701 ggtgggccgc gatctgcaaa tcttcaactc gctgaaagcg aatatggcag attacctgct
     2761 acttgatggt gagttatgcg ccaacgtgca gggtaatttg atggatgaga tgctgattac
     2821 gattattcag gggcacgctc agcgactcgg gatgaaaacc atcgccgggc cagtcgtttt
     2881 acccttagtg atggatacgc tttctggcat cggcgtcgat ctgatttatg gtgatgtgat
     2941 tgccgatgcc caaccgctgg atttgctggt gaatagcagt tatttcgcga ttaactgaga
     3001 gagcgtttgc ccattgcctg atgcgatgct aacgcgtctt atcaggccta caaatcgctc
     3061 attccgcagg cgggataagg cgctcgcacc gcttccggcg accaacgtca tgcttcgtct
     3121 ggttgccagc cttccgtata ccagatatgc aacagcgcat aagaacgcca gggcttccag
     3181 cgctcggcat aacggcggat ttgtgccggt gtcatcccca gaaatcgctg tttaatcaga
     3241 taatcatccg gcagaaaaac atctttcgcc tgccagccgc gcaaggcgaa ataattcgcc
     3301 gtccagcgcc cgatacccgg aaaagtttgc agcgttttca tcgcctgctc cacatcgccc
     3361 ggtattgtca ttggtaaggt gccctccagc gccgcatttg ccagatgaat cagcgcctct
     3421 gcccgtttca acggcatacc taacgctttt aatgcctgcg ggtcggctgc tgccagccgc
     3481 tgaggcgtgg ggaagcagat atactccgga aaatcatcca gccgttcgcc ataaagctgt
     3541 gccactctgg cggtcaattt tgccgccatc gccacgctca ccagttggcc taaaatcgcc
     3601 cgcacgccct gctcaaaagc atcaacactg ccgggtaaac gcaatccggg ccgcgccgcg
     3661 cctaacttgc ccaacgcacc gttaacaatc tgcgggttac attgcagatc aagcaggcgg
     3721 ctcattttcg ccagacactc agcggcaacg ggttctaaac ctgcacttaa atttatgtgc
     3781 agagtatggc gggctatatc cggaatagca gtcaccacgc cgcgatattc gcccactgcc
     3841 agactacggg cataataact gtccgcgacc gtttccacac cactcaccgc acgggcggcg
     3901 agaaatccca acatccacga ccagtcatac ggcggctgcc agttcaggat gtacatcgca
     3961 tctccttatt catccgcttt cagcataaac gttattcaga cgttacgctt tgctttcata
     4021 ttccggttgt cgcgacggca acatttcgct aaagtcacgc cccttcttca ccggcatggg
     4081 gattatttcg tgtttattgg ttttgattac ggtacagcaa actgttcagt ggcggtcatg
     4141 cgtgacggta aaccgcattt gctaaaaatg gaaaacgaca gcacgctgct gccttcaatg
     4201 ctttgcgcgc caacgcgtga agcggtaagc gaatggctgt accgccatca tgacgttccg
     4261 gcagacgacg atgaaacgca ggcgttgtta cgacgggcga ttcgttataa ccgcgaagaa
     4321 gatatcgatg ttacagcgaa aagcgtgcag ttcggtcttt cctcactggc acagtacatt
     4381 gatgatccag aagaagtgtg gtttgtgaaa tcaccaaaat cgttcctcgg tgccagcggc
     4441 ttaaaaccgc agcaggtagc gctgtttgag gatctggtct gcgcaatgat gttgcacatt
     4501 cgccagcagg cgcaggcgca gctgccagaa gcaattactc aggcggtgat tggtcgcccg
     4561 atcaacttcc aggggctggg cggcgatgaa gcaaacgccc aggcgcaggg gattctggaa
     4621 cgcgcggcga agcgtgccgg gtttaaggat gtggtattcc agtacgagcc ggtagcggct
     4681 gggctggatt acgaagccac cttgcaggaa gaaaaacggg tgctggtggt ggatatcggc
     4741 ggtggtacga ctgactgttc attgctgctg atggggccgc agtggcgttc gcgtctcgat
     4801 cgtgaagcca gtctgctggg tcacagtggt tgtcgtattg gcggtaacga tctggatatc
     4861 gccctagcgt ttaaaaacct gatgccactg ctgggtatgg gtggcgaaac cgaaaaaggc
     4921 atcgccctgc cgatcctgcc gtggtggaat gcggttgcca tcaacgacgt tcctgcacag
     4981 agtgatttct acagtagtgc caacggtcgt ctgcttaacg atctggtacg cgatgcccgc
     5041 gaaccggaaa aagtggccct gttacagaaa gtctggcgtc agcgtttaag ctatcgcctg
     5101 gtgcgtagcg cagaagagtg caaaattgct ctttcaagcg tagcggaaac ccgcgcctca
     5161 ctgccgttta tcagcaatga actggctacg ctgattagcc agcgagggct ggaaagcgcc
     5221 ctcagccagc cactggcgcg gattctggaa caggtgcaac tggccctgga taacgcccag
     5281 gaaaaaccgg acgttatcta cctgaccggc ggtagcgccc gttctccgct gattaaaaaa
     5341 gcgctggcag aacagttgcc gggcattccg attgcaggcg gcgatgactt tggctccgtc
     5401 accgccgggc tggcacgctg ggcggaagtg gtatttcgtt aaaagaaaat ggctcagcag
     5461 cggcttaaat cagcctgcgc ctgagccagt ttttctgccg cctgacgtaa cggttccatc
     5521 aacggcatgg tccgtgctgg agcatgaagg cggaatcgct gcaactctgt cgcgccgaca
     5581 gtcaacgtcg attccagccg atgtcgtttg gcggccattt tcgccattac cgcctgtctg
     5641 tctgctggcg ttaccgcttc gttcggcctg aacacaaaac ggcgttcaca gctcgctttc
     5701 cagtcgatga ccgcctgggt cagatgatca ccaaaccctt taacttgctt aacgccacga
     5761 cgggtaacac ctgctgctgt ttcaatacca aaagaccata acgccgcttt acgcgcaggg
     5821 ccaacgccgg gaatagaggc aacatcaata aaaaatccct ccagaaactt ctacttctgc
     5881 cgttcccttg cagtgtcatg aagtgctgcc agagcgagtt tctcttcttc cggtaacccg
     5941 agaatttcgt ccttcatttt ttcgagcatc gtccgtttgg cgataaaacc ttccaggccg
     6001 cctacctgct ggatctggcg gactaaatgg tcatagtcca ttttcgcacg attaaatgcc
     6061 tcacggcgtt gctgaacttc tgctttgtac gctttgcttg tcagactgcc aataatccag
     6121 atagccgcca gcacaggaac caaaataata taacgcggtt ctgcctgaag gccgcaaagc
     6181 agcagcgata atgctgacag tgcgatctca agcagaatga tgtattcgcg ccgtaacagg
     6241 cctaaaggaa gcggcctgcc agtcggttgg aaatgatcgg gtaatggcag ttgcaatgct
     6301 ggcggtgcta ctgacgccat caccatcgcc cagacttttg ccagcacaaa atccccgccg
     6361 gtggtaatga cctcttcgcc gagatcaata aaatagataa cgccttgatt atccagcgta
     6421 caccacaggc agtcggtcaa atgagcggga taaacatgca ttgccgaaac gggacatttc
     6481 tttaactgtt ggcgcagaga atcaagcgcc gctaccctcg ctttagccgt cggacgcccg
     6541 gttgccacgc cactttccgt gaatgcctgc tgaaacatgg cttcaacatc actcggtaac
     6601 atcgatagcg gaatcgatcg tggcggcggt tttaaaccac gtcgctgatt atctgacgcg
     6661 taggcataac ggaaatgggt aatatccgtc tccagcgcat tacccgcatc agagataagc
     6721 ggcacaccag aataaggatg ccggccacca aacaagacgt gaaaaatcag caacgcaagg
     6781 ccaaaattac cgtgattttc tgtgcgttca aagccgacaa atgacgacaa ggtttgtagc
     6841 tctggcggcg taaaatgcga cacgccgact tcgcataaat gcagtgtgcc attggcgtta
     6901 atctgaaagg agtcgctatc aatcaacacc actttgctgt cgcgacctac cataaaactg
     6961 ttctggttta cgtcacccac gacatgcccg tgctcgtgaa ccgtagcaaa agatgaagca
     7021 atattgcgcg caacatagag tagaaaatcc cacgcacaat gagggtaacg ttgacgacga
     7081 tgtgccgggc tatagatcat atgaatcggt tctttaccag aaacttttgg catcataaaa
     7141 ccgataactt ttccgcctcg tccaccgtga agcgttgcct gcggccaggc gacataattc
     7201 aacaattgcg cgtcagctgt cgcagccata aaggcaagtt tgtcctgttt taaggcgggt
     7261 ggcggcgtgt gataaatctt ggcgacgcta tcgacaaact cattgatatc ataaaccgcg
     7321 ccttcaccgc ctttgcccag ttcacggcct ggcgtaacgc actcgccagt agcagtatat
     7381 aaagtgggtt tcatggtaac cacagcgcca gtgccaacgt tttatcatca tccgtacgct
     7441 cgtttaccgc cgggctggac aaaaactgct ttaacaattc aggtaataaa tcgagttgtt
     7501 cctgcgttgc tgacgccagt ccattgaaaa acggggtaaa gaaaggcaca tgcggagaat
     7561 tatccaacat atttaacgcc agtcgctgga taccatccgt aaatgctgca actttatgca
     7621 cacgctcagt gctggtgaaa gtttccagtc tggaaacggc atcttcatcg gtaatgaagt
     7681 gcgtcatatt ggcatattca cccaccatcg gcgtaagcgg caattgcaga ccgtgaccaa
     7741 aatccaccac cacgccgcca tcgccaatct gcataatcaa ggtgccatta gccgatgaaa
     7801 tcaatcccag aaacgtacag gcaaaatcgc gaactgctaa ttctttggct tccgcctcgg
     7861 caataaagtc gctgacgaac ggtcagcaca atatccgtgg cgaggatatc attaagcccc
     7921 aattccccac cctgcacttt ttgcgacata taagccatcg cttcattgac ggcgagcatc
     7981 gcgccttcgc ccccctgcga aacgctacct gcgccatcag caaggaacat caccagtaat
     8041 ggctgctggt catttagcca ggcaacctgc atctggcagg catcctggca aggcaagtcc
     8101 gcgctgatgt gcgacgttcc aacagcggat gcataaacca gacgccagct cacactgacg
     8161 tccagccttt tggcgcttcc agcacaactt ccgttcccgg cgtggagcgg gaaaccgaac
     8221 gcagcgaact ggataaccag ctaaacagtt cacggaattg taatccttgc agaggcaaag
     8281 gctgacgaac gctgatttgt gccagagtct tcatatccgc cccctgaaca ccgatggaaa
     8341 agaaagcaaa ccttttatct tcttcccccc gaaacacttt gttggctgca gcctgccact
     8401 catcggttgg tgcaccatcg gtaatcagga aaatccacgg gcgataatac gaaataccat
     8461 ttgcacgata ctcacgtttc cgctcctcta ccatatccag ggctttggta atggccgcac
     8521 ccattggtgt atcgccctgg gcaaacagga tgggcggaaa aaaattagcg gcgctggtaa
     8581 agggttgttc cacatgcacc gggccgaacg tcacaatgcc gagttccact ctttttaaag
     8641 ccagcgaatc ggcaagcagc tcatcgcgaa aggtaaccaa tccggtgtta agttcattaa
     8701 taggtctgcc actcatcgag ccagaaacat ccagcagcag aatgcagggg caacgtagtt
     8761 ccgggttact ggcaaaatcg ctggtggcaa acgtgatttg ttcgctcata agaaatcctt
     8821 tcttagagaa ttattgaggt ccggtatcat accgactgca ataatgaaat aagagcaaac
     8881 gaagaaactt tcagaattag tcccggcggc gcagaaaatt ttgctattcg cctcgcaaga
     8941 acggagagcc gtcgattgac actaagggcg gagtgacata atttcaggag tgagggttag
     9001 ggagagg
//
LOCUS       DAETPZ010000131         8889 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.132, whole genome shotgun sequence.
ACCESSION   DAETPZ010000131 DAETPZ010000000
VERSION     DAETPZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.132"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..220
                     /locus_tag="K0W59_003663"
     CDS             <1..220
                     /locus_tag="K0W59_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="nucleator protein that participates in the
                     polymerization of curlin (CsgA) subunits into curli
                     (extracellular fibers from Escherichia and Salmonella spp.
                     that are involved in the colonization of inert surfaces
                     and biofilm formation); part of the curli secretion and
                     assembly protein complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curli assembly protein CsgF"
                     /protein_id="HBI3024528.1"
                     /translation="ALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIVDIANRDGQ
                     LQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            247..1022
                     /locus_tag="K0W59_003664"
                     /pseudo
     CDS             247..1022
                     /locus_tag="K0W59_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(1086..1577)
                     /locus_tag="K0W59_003665"
     CDS             complement(1086..1577)
                     /locus_tag="K0W59_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBI3024529.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(1679..2233)
                     /gene="ycdY"
                     /locus_tag="K0W59_003666"
     CDS             complement(1679..2233)
                     /gene="ycdY"
                     /locus_tag="K0W59_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HBI3024530.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(2257..2994)
                     /gene="ycdX"
                     /locus_tag="K0W59_003667"
     CDS             complement(2257..2994)
                     /gene="ycdX"
                     /locus_tag="K0W59_003667"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HBI3024531.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(3049..3987)
                     /gene="ghrA"
                     /locus_tag="K0W59_003668"
                     /pseudo
     CDS             complement(3049..3987)
                     /gene="ghrA"
                     /locus_tag="K0W59_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            4221..4308
                     /locus_tag="K0W59_003669"
     tRNA            4221..4308
                     /locus_tag="K0W59_003669"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4255..4257,aa:Ser,seq:gga)
     gene            complement(4458..5300)
                     /locus_tag="K0W59_003670"
     CDS             complement(4458..5300)
                     /locus_tag="K0W59_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HBI3024532.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELIDRLNDIIAKHYPD
                     MLAV"
     gene            complement(5385..5582)
                     /locus_tag="K0W59_003671"
     CDS             complement(5385..5582)
                     /locus_tag="K0W59_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024533.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(5594..6085)
                     /locus_tag="K0W59_003672"
     CDS             complement(5594..6085)
                     /locus_tag="K0W59_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024534.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(6082..6456)
                     /locus_tag="K0W59_003673"
     CDS             complement(6082..6456)
                     /locus_tag="K0W59_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HBI3024535.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(6503..6877)
                     /locus_tag="K0W59_003674"
     CDS             complement(6503..6877)
                     /locus_tag="K0W59_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HBI3024536.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPALATTS"
     gene            complement(7040..7261)
                     /locus_tag="K0W59_003675"
     CDS             complement(7040..7261)
                     /locus_tag="K0W59_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HBI3024537.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(7324..7800)
                     /locus_tag="K0W59_003676"
     CDS             complement(7324..7800)
                     /locus_tag="K0W59_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HBI3024538.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(7816..8301)
                     /locus_tag="K0W59_003677"
     CDS             complement(7816..8301)
                     /locus_tag="K0W59_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBI3024539.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(8392..>8889)
                     /locus_tag="K0W59_003678"
     CDS             complement(8392..>8889)
                     /locus_tag="K0W59_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HBI3024540.1"
                     /translation="TLQETRGGSGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEG
                     AYEVVGVFDRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRR
                     EDYGKDLWSAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETL
                     LESMR"
BASE COUNT         2194 a         2285 c         2255 g         2155 t
ORIGIN      
        1 agcgttagat aactttactc aggccatcca gtcacaaatt ttaggtgggc tactgtcgaa
       61 tattaatacc ggtaaaccgg gccgcatggt gaccaacgat tatattgttg atattgctaa
      121 ccgcgatggt caattgcagt tgaacgtgac agatcgtaaa accggacaaa cctcgaccat
      181 ccaggtttcg ggtttacaaa ataactcaac cgatttttaa gccccagctt cataaggaaa
      241 ataatcatgc agcgcttatt tcttttggtt gccgtcatgt tactgagcgg atgcttaacc
      301 gccccgccta aagaagccgc cagaccgaca ttaatgcctc gtgctcagag ctacaaagat
      361 ttgacccatc tgccagcgcc gacgggtaag atctttgttt cggtatacaa cattcaggac
      421 gaaaccgggc aatttaaacc ctacccggca agtaacttct ccactgctgt tccgcaaagc
      481 gccagggctt acaaaacctg cttaacgagc gcaaggttat tcgtgcggca caagaaaacg
      541 gcacggttgc cattaataac cgaatcccgc tgcaatcttt aacggcggca aatattatgg
      601 ttgaaggttc gattattggt tatgaaagca acgttaaatc gggcggtgtt ggggcaagat
      661 attttgggat cggtgcagac acgcaatacc agctcgatca gattgccgtg aacctgcgtg
      721 tcgtcaatgt gagtaccggt gagatcctct cctcggtgaa caccagtaag acgatacttt
      781 cctatgaagt tcaggccggg gttttccgct ttattgacta ccagcgcttg ctggaagggg
      841 aagtgggtta cacctcaaac gaacccgtta tgctgtgtct gatgtcggct atcgaaacag
      901 gggtcatttt cctgattaat gatggtatcg accgtggtct gtgggatttg caaaataaag
      961 cagaacggca gaatgacatt ctggtgaaat accgccatat gtcggttcca ccggaatcct
     1021 gacagataaa aatgccggaa ttaataatcc ggcatttact gtttaagcga aagcgtgagt
     1081 gcccctcacg ctttattttt gatttcctgc tcacggtgag ccgtctttgc actacgcgcc
     1141 gccagccaca ggccgctgtt tttcattgcg taaccaaata ccagcccaag cgccagcgaa
     1201 ggtaacacca gtttccagtc accctgcccg gcaaacgtcg cgcatgcgcc tataaacgtc
     1261 cccggaacaa atgaaagcag cagctgtttg gcctgaatac acatcagaaa cgcgacaata
     1321 ccggttatga cataaccgag aatttccaga tgtggtgcca gtgcactacc gtaaataatg
     1381 accatcgccc acaccacgcc actaagcagg gttgcagcgg agatcgccag ccctttcagc
     1441 ccaccttgcg ggcaagcaaa gtaggccgta cagcccagga agcccgccca gcttagtaag
     1501 cccagggaaa cagccaccca tccccagata ccggagagaa tgcctgttgt gattgcaata
     1561 gagagaagta tgttcatgcc gcgcatgata gcagaaaacg cggccatgaa tgagatcgag
     1621 cacacatttt atgcaacaac aaattatctg ttgcagttat gagtgacgat gatcacattt
     1681 actcttcaga atcttcttcc agctcgtccc acattgcact aatggcatca cgggttagcg
     1741 gtgccatggt gcgccagaaa ggcgtggttg catgggcctc cactttgccg aggaacgcac
     1801 cacaccaggg taacagatac tcactgaaca gtgtttccag tgcttcgctc tcatcttccg
     1861 ttgactgatc ttccagccag gaagccgcaa gcagcaatgt gccgatgtga tcggctggcg
     1921 tatccgccag tggcatccct cgttcggaaa gaaaagcgcg cacttccgct tccgttgcgt
     1981 cctcaaccca tgcgctacga tatggcggca cggcacattc atcgccgata aacaacgcat
     2041 tgtaatcggc agagacttgc gtcatatcac agcttttctg taaacgcgtc agtaactcat
     2101 cctgctccag tggccagttc gcagccagtt tcccctcacg aatcagagta aacagcggca
     2161 ccagtaaagg atcttgcggt tggcggtaat agagcgaacc cagcacacga cagaggatag
     2221 aaaactcgtt cattaatata tttccattac tagtaattaa aggtctgcaa attccgcaat
     2281 cggtgccata ccgcgagatt caaggaagtt cagtaagcgg cgcggagaaa cattcaaaat
     2341 gcgctctggc ggaaaatcta ccgcgtcgag gattttaaga cactcttcaa attcccccat
     2401 ggtaaacgcg gtgtgagaat ccgagcctaa tgccacccaa cctccagcat cacgtaccgc
     2461 tgcagctacc gcacgacagt tgtcttcact gcccttacgt gagtgtaaaa atgaggaatt
     2521 attgatttcc agcgccacct gatgtttcgc ggctgcttca gcaacggctt taacatctat
     2581 ttcatatttc ggatttccgg gatggcttat tatatgcaca ttgccgctgg cgatagtggc
     2641 gatcatcgct tgtgtattgg tcgctttgtc atgtggcgca aaaaccggct catgaaaacc
     2701 ggcaataatc agatccagcg agtcaaacat tttaccgctg cagtcaattt caccatcaac
     2761 atttttaatg ttagcttcaa tgccgcgcag gatccctacc ccatcaacca ctcgcggcca
     2821 gatacgcatg ttaatgaagt gccagtgatg cggcgcatct tccatatccg ggccatgatc
     2881 ggtgatcgca aaaagtttaa tgcccttttg tttggcctgg gcaatgtaat cactcaatgt
     2941 gctatatgca tgtgtgctgg caacggtatg catatgaagg tcgacgggat acataactct
     3001 ctcctgtatt caattcccgc caaggatagc aggaatcctg atgctttatt agtagccgcg
     3061 tgcgcggtcg acttgcccgc agaccctctc ccctttttcg agctgggcaa tagtgcgaga
     3121 aatgtactcc acagcttcag cgggacgggt aatcgcggcg acatgtggtg ttatcgtcac
     3181 gcgtggatgt tgccagagcg gactttcagg cggtaagggt tcacgattaa aaacatccag
     3241 cattgcgcct ttaactttcc cgctatccag cgccgcgagc aggtcatctt ccacaacatg
     3301 aacaccacgc gccaggttga ggagatacgc gccatcaggt aatttttcga gtaattgttg
     3361 attaataatg ccgacggttt caggggtatt cggtaacaaa ttaatcaata cccgacattg
     3421 gctcagaaat gcagacagtt cttcccgtcc ggcaaagctt tgcacgccag gccacgattt
     3481 acgggttcga ctccagcaac gcagcggaaa gcgctaggtt tgcagactct gagcaacttt
     3541 acagcccagt acgcctgcgc ccaaaatgcc gatggtaaaa tcttcccgat gatattcagg
     3601 cagcggttgc caatgcgaac tattttgctg gatgcgataa tcatcaaaac gtcgaaacca
     3661 atgcagcacc tgactgacag catattcctg catttgctcg cccataccgg tatcttccag
     3721 gcgaaaaagt ggaacagaag ggttcagcat ttcagggtgt gcctgtagct tgctcaaaat
     3781 agaatcaaca ccggccccga gtgcgaacac cgctttaaga tcgcgccctg ccagcatttc
     3841 aacaggagga tgccagacta aaacataatc agcagagtca ttatctccgc ttttccatgc
     3901 cctgactctt gcctgaggaa tagctttgcg cagtgcctca atccaccatt gggtatcgaa
     3961 cgttgggtga taaaagatga tatccatact gactcccgaa aagcgttgtg cgaaatttat
     4021 tcgcacttat cgttatgatc tacaaaggcc accagcataa caaatccgtg gtcggtggca
     4081 aaaaaagcag atttcgctta ttaaaaccac acattgattg aagtttgaat aaacgcgcga
     4141 ttctttcaaa aagtttgttg acctcaggtc atgatttccc taaattagcg cccgttccag
     4201 caagacagga acgacaattt ggtgaggtgt ccgagtggct gaaggagcac gcctggaaag
     4261 tgtgtatacg gcaacgtatc gggggttcga atccccccct caccgccaga ttataagcgc
     4321 atgttattgt ttaacatgcg cttttttatt ttcccttcca gacagtatgc catcgggtat
     4381 gccatctgaa accggtttat atgccccgcg atcatatctt atcggacaat ttccgatctt
     4441 tgagcatact ggcaaagcta tactgccagc atatcaggat agtgttttgc gataatatca
     4501 ttcagtctgt caatcagttc tggttttctg aacgtgatat gcgcggagcc cttcttaaag
     4561 tatctgatgc tgaacatctc atcttcatag ctctctttac cctgaacaga ctgaatgtga
     4621 tcatccagac gaagggtgat gtttccccgg ttgtcgggga ttggtttacc gctgaacagg
     4681 tgcaacattc tttccagatc ggcgagtcgg tcagcctgtt gtccggtgat aagatgaaat
     4741 ccccaccggt cccatctcac cagattgttg acgataattt tactgccaaa tttacagggg
     4801 caattggttt tgtaattcca gttcagccct ctgaagacgt tgatcacccc tcgctcaaac
     4861 acctcatcct tattctggtg cagttgttca aatgtgctca gaatgttcgc ttcgctgatt
     4921 tccggaaaat tatcgtactc cagtgaccgg taccatgtat cacgggcctg cgtatccatt
     4981 aatgacagca tcccggaacg ttgcatcagg tcacgccaga tttcgcgatc aagattccgg
     5041 gtaatggctt tcattgctgt ttcgggtttc tccatcagcc agcagccgca gcgaaaatcc
     5101 tgtttcatgg cccagtccgg ggcagtttta cctccgatgc tttccgtcag caccgagatt
     5161 tcagccagtt gtcggataag tgtgtcgatt tgcaacagtg tactctttcg tacagccaga
     5221 atgtggcgaa tgctggttga gcagataacg tccgtatgcc cgttgaccac ttccggacgg
     5281 gagattgtga tatctgacat aatcgatatt cctttttgca gataaatgaa aacgccagcc
     5341 ctgtgcaggc tggcgtcaga ggaatgaagt ctggtgagtg agcttcaccg gttctggtgc
     5401 agaagctgca gatgacgcag ggtacggaca tcctcccggg cctgttcgat acagggcaac
     5461 agtgctgctg aatctgtttt atcctcatcg ttgtcaaaga taattcccga tccgcagtcg
     5521 atattgtcct gcagccacgc aatcagaata tccagcgctg tttccgtggt taatgatttc
     5581 atgttgttcc tggttaccag tcgaaagtgg gtaaacccgg caggcattca gcgctggcat
     5641 ccagatgaat gagactgacg ccataacgcc ggatgagtgt gacgaccaga cgacggaacg
     5701 tttttgacag ccccaggcgt ttaagccgca gaaccgggta tgaccacgca tcggtacgta
     5761 acagatatcc gctacctgta aaatgaatcc attctgattc accgaagtca ctggtctggt
     5821 gtgacagcga gtacaaccag gcgttgtccg tttccgtgat atgtgcggta ctgcagcgta
     5881 tgccggtaag ggtagcaaat ggtggcgtgc attctgctga ggtctggtca gattcatcaa
     5941 caacacgaag ggagtagccg ttatcgcaga ccatgttaat cagttcagcg agctcaatac
     6001 catcgacgtc aacgacaatg cgccccatgt tcagtgcctg tacgttaacg ctgtcggctt
     6061 ccagtgtcag ggccagtttc atcatttcgc ctccggatac ttacccaagg taatgttatt
     6121 taccgttctg taattgtcgc gggtcatcag gccggtcgcc cggcgagccc ggaggatatc
     6181 aatactgttt attaactgag agcggggaca ggtgctgaat cccggctggt cggtacgcac
     6241 cagcgcgtat ttttccacga gaaaattaac tgcatcacac agtgaaatgc ctgcctcaat
     6301 atgctgctca atcacacgtt catcggcaaa cggtgtgtca ttcagtgtga ggccataatg
     6361 ctggtccagc agtcggatga gcagtgtctg ccagatggtg acgggagacg ggcagcgaga
     6421 tacctcccgt acatgtgtgt cggataatgt tttcatctta agttctctgt aactggtagt
     6481 gctaaaggtg agagcttcgg ggttatgagg tggttgcgag tgctggtgtc ggataaacag
     6541 ccagataaac gtaaccacag gagccgaggg tgtcggcttc gcaggtcagc ccttttgcat
     6601 tcagcgtgac ggtatgctga tatcggggat tcagttcacc gctggtgagc atgagttcca
     6661 gttgtttcat cagcagcgga aaggcctggt ccagatggta tgcatctgcg tcgctgaagc
     6721 ggcctctgat accggcgcgg tcggcaaggt aatgcaggcg gttaccctct tgcaccagac
     6781 gagcgccaaa acagggcctc actgtgcagg gaagccccca ccaggggcgg tcgtgattgt
     6841 catcgggatg agttatcccg gagagtttgt ctgacacgat aaaatcctct ctgaaaagtg
     6901 gttaagaatg gtttaacgat gacaggtcag ccggcgcgct gaccggtttc ggtgaagatg
     6961 tatgcattga tgagaatggc ctcatccaca gcggcgtctg aggtcatctg gtcgtactcg
     7021 tttcccgggt gacggaacat cagttcaggg tgacactcat cagccggaca tacgggccaa
     7081 aactgtcctt acggcgttct gcaaacacgg caagaacgcc gggaatatcc tgcacttcac
     7141 gaccagtata cgcttcagca ctgccgtgcc agcgatactt accggtacag aacggaaaaa
     7201 gacgggatgt cggatgttgt tggtgaatac gcatggcttc accacgggtg ataattttca
     7261 taatgggata cctctgaaga cagaagataa aagtgaaaac aggtgtgatg tggttgtgaa
     7321 gggttaaagc agaccatgtt cggcaaagga gaaaacctgg ttgccaccga ctatcagatg
     7381 gtccggcacc cggatatcca ccaggcccag tgcctgtacc agacgttcgg tgataagccg
     7441 gtctgcctta ctgggtgtga cttcaccgga cgggtgattg tgcgccagca ccacggcagc
     7501 ggcattgtgg tacagggcgc gtttaatcac ttcccgggga tgaacttccg tacggttgat
     7561 ggtgccggtg aagagggttt caccggcaat cagctgattc tggttgttca gatacagcac
     7621 ccggaactct tcccgctcca gtcccgccat gttcagaatc agccattccc gtgccgcacg
     7681 ggtggaggtg aaggccacgc cgggttcatg aagatggcgg tccagggttt gcagggcccg
     7741 cagaatgaga ctgcgctcgc cgggcgtcat ctctccgggc agaaaggaaa gctgctgcat
     7801 tgtccctgcc tccattcagt caatgatgcg cataatggcg ctgcattccg gatgctgcag
     7861 ggcgtaatcc cgcagccggt aatagtggac cgtcatggca taacactccg tacgacaggc
     7921 atgatgactg tacgtcatca gacaggcagc aataccggcg gcttccgggc tcatttcagc
     7981 gcggttgccg ttcatggcat tgaacagtac ccatgtttcg tcatcatcgt catccggttc
     8041 gggtgccata aatgccccgc cgttgttcag ggtgtacaga ttccagatac caccgcagta
     8101 gtcttcgcaa agacggtcca tccagccgaa gacacggggc tccagggtca cccactgtgg
     8161 aatgaggcca aaatgctgcg gccagaagct gatgcgctgt tcatcaggga cgggagtggc
     8221 aaccagctga ggtgggttat tccctgatgc agtggttacg gaaacagaag gtgtggtgga
     8281 attatgcgaa acggttgtca tgaggttatt ccttataaaa agtaaatgaa tggaagaagc
     8341 cccggggcag gaacagatgt gagtcaggac tgcactttca gtgaaacggc atcagcgcat
     8401 gctctccagc agcgtttctg ccatcaccca caatgcgcga ttgagcttaa tgtcggtatc
     8461 aatgctgtga atggcccggg tatggatacg ttttcctttt gcactgcgac cggaaatccc
     8521 gcctttcagc atattctcct ggatggtctg ataagcactc cacaggtcct taccgtaatc
     8581 ctcccggcgt cgtggtgtca gaatgtcggc ggtggtgacg ggctgatgtt cgtcaccata
     8641 acggtaagtc agtgccgcct gtgccagcgc ctggcgtgcc ggtggcggca ggaccagcga
     8701 ctgcatggca tcacgcttct cctctatccg gtcaaaaacg cccaccacct cgtaagcccc
     8761 ttcaatgact ctgtccacca catttccccg gtgtggaaca cgcaattctc ccagagactg
     8821 accgcagacc agtccattgg tacaaatggc acgaaaatat cctgaaccgc cccgggtttc
     8881 ctggagagt
//
LOCUS       DAETPZ010000132         8866 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.133, whole genome shotgun sequence.
ACCESSION   DAETPZ010000132 DAETPZ010000000
VERSION     DAETPZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8866
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.133"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(102..1181)
                     /locus_tag="K0W59_003679"
                     /pseudo
     CDS             complement(102..1181)
                     /locus_tag="K0W59_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
     gene            complement(1391..1834)
                     /locus_tag="K0W59_003680"
     CDS             complement(1391..1834)
                     /locus_tag="K0W59_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024541.1"
                     /translation="MNPSDAIEAIEKPLSSLPYPISRHILEHLRKLPRHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSALMTVLPGHAAIPLMTRLQDELRTESVRTQTREQFTG
                     AVDRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFFLTR"
     gene            2349..3191
                     /locus_tag="K0W59_003681"
     CDS             2349..3191
                     /locus_tag="K0W59_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024542.1"
                     /translation="MKKELFGRCPFDLDEDVKKIAAINKYIHAKFEEHKNKIDNLKGV
                     EQKLMVLQYFNVLNELSNQAISSLNIGAFSASEVLARVIIEQAANQFYIAIDDGENAQ
                     ALLKASKKLVHSNGKRWLECLKSKGMTNPAANERIRMGKELTDLFNRLWPNTPEYPGT
                     RKLFELIGWETHYHAHYVPLCDSIHTFSDDMVNIVSLYNAIQSDETTAIEFILAVKQE
                     NKRLAIYNFVIATALRCEALVNVFNSLGYQDIITEMTPTIDTVNQTIIRYDDFDHSRI
                     DDCQ"
     gene            3298..3561
                     /locus_tag="K0W59_003682"
                     /pseudo
     CDS             3298..3561
                     /locus_tag="K0W59_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            4188..4733
                     /gene="cobU"
                     /locus_tag="K0W59_003683"
     CDS             4188..4733
                     /gene="cobU"
                     /locus_tag="K0W59_003683"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBI3024543.1"
                     /translation="MMILVTGGAWSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            4730..5473
                     /gene="cobS"
                     /locus_tag="K0W59_003684"
     CDS             4730..5473
                     /gene="cobS"
                     /locus_tag="K0W59_003684"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBI3024544.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSCDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            5485..6564
                     /gene="cobT"
                     /locus_tag="K0W59_003685"
     CDS             5485..6564
                     /gene="cobT"
                     /locus_tag="K0W59_003685"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBI3024545.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            6626..7561
                     /gene="ldtA"
                     /locus_tag="K0W59_003686"
     CDS             6626..7561
                     /gene="ldtA"
                     /locus_tag="K0W59_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBI3024546.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFASQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(7617..7692)
                     /locus_tag="K0W59_003687"
     tRNA            complement(7617..7692)
                     /locus_tag="K0W59_003687"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7657..7659),aa:Asn,seq:gtt)
     gene            8019..>8866
                     /gene="nac"
                     /locus_tag="K0W59_003688"
     CDS             8019..>8866
                     /gene="nac"
                     /locus_tag="K0W59_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HBI3024547.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAV"
BASE COUNT         2258 a         1973 c         2258 g         2377 t
ORIGIN      
        1 tcccacaggt ctgtcgcccc ttcagttgcc agttcgctgt ctttacagac gtaatctttt
       61 ttcatgcgac cgaaggcttc cctgacaaaa tcatcgacct aggcttcatc aacaggaagg
      121 gttccgtttt tccgtaactc atgaagcgca cggttagcca ggtatgcctg gcgtgaggat
      181 ccgggataaa cgcgggaggc gggcagcaca tcgctgttca gcattgccgg taccttctga
      241 cagactgcat cagccccatc gccattacgg tctttttcat caaatttatt gaccagcaca
      301 aacaggcggc cgactgacac atcagcaatg gcattaagct ctttacgaac ctcctcatcg
      361 gctttcgagt tcatctgggt gtagtccatc accgccagca ccgcagaggc tttctgcagt
      421 tgatcccgca tcatcacttc catctgcggt tgtccggctt cattagggcc gggggtatcc
      481 accagtgtga gtgttccctg gcattcatcc atccccacca gatggctgaa ttccacgtcg
      541 atcaccggca gtttctgcac ttctgcgtat tcatcaaaag gaaattcact ccccattgcc
      601 gcagcaagac gaaccagatc gtttaacgat gccagtccgg taaagattcc ctcctccccg
      661 tggtattcat ttttgagcca gccatcatcc gtcagaatat ccagcagttc gcgggtatcc
      721 ccggtttgct gcagggtctg tgcgacctgc tgtcctgccg gggtggcgac tttttcctgc
      781 agtgtgatta ataaattgcg gacaggctga atatgttcca gatggagaac cggctcagtt
      841 tttccgggaa cgtggcggat gagcgtcggt acagaggtca tggggcggtt acggttaggc
      901 agaatttcct gccccacaat ggcgttgatg gtggttgatt tgcctgcttt catcgtgccg
      961 acaacagcca gcaccatctc cctgcgggcg gttttgattt gttcgccctc cagctcctta
     1021 attcttttca gtgctttctg tttatcaaaa agctgcccgt tttcgttctt accgtcagat
     1081 aacacatccg gctcatcaac catttgccgg agcagattga tgctcagttg caaaagccgg
     1141 tcggcttcat cacaaagcag ggtgatgttc ttttcgtgca ttatttaacc ttcctgagac
     1201 gcattttcat gacaaccacg gaggaggcca tttactacaa gcgatcgttt ataacaatca
     1261 atataataga aacgatcgtc atattctatt aatatgaaaa acacaacgca agggggacaa
     1321 cccgggtatg gctgcgtttg cattgtgttt tcatagagtt aaaaatctgt aacgacaacc
     1381 cggcacatca ctaccgggtt aagaagaaaa tccagttcca taccgcgcgg gccacagaga
     1441 ccacggtgtc acgaacggcg cgcagggccg tacggacaac agaggcaaca cagacactct
     1501 ccgccgtgtc aaatatccgg tccaccgcac cggtaaactg ttcacgggtc tgagtgcgga
     1561 cagactccgt gcgcagttcg tcctgcaggc gtgtcatcag agggatggcg gcatgtccgg
     1621 gaagcactgt catgagtgca ctgaccgggg tgacctcccc ctgaaacagt gcattacaga
     1681 gtgaggattt accggccccg cttttaccca taatgccagg cacgggttcg tgacggggga
     1741 gtttgcgcag atgttccagg atgtgacggg aaatcgggta aggcagggag gagagcggtt
     1801 tttcaattgc ctcaatggca tcagacggat tcatacatgt attcccagtg aaaaaagaca
     1861 aaaccccgtg caccggaaag gtggcggggg attcacggag ataatatctt tctgaaatta
     1921 ttttgaatgc aggtatatgt ggctgatttg tatcagctaa tgagtacagg tctgaatcta
     1981 atctgacagt ccgctttgtg ccaggagcgg actgacctgc tccccgatga ttaatacaca
     2041 gctatgctag taatgtctgt agatcgctgt gtgttgcatc tatcggttga actcacagtc
     2101 caaaccagac tgtcagcttt gattagattc tacctactca acctgtcaga taaggtctga
     2161 gctaatacat ctaatatatg gctttgtgca ggggacggct atcgcgaacg ttaggctagg
     2221 tatctgaata cgcacagttc acttggttgt acttcttgca ctgatctgct atcccgcaca
     2281 gctatttata ggtaatcttc agtgtataat ctgccgactt atggaagctt gtacaacagg
     2341 agaaatatat gaaaaaagaa ttgtttggac gttgcccgtt tgatctcgac gaagatgtaa
     2401 aaaaaatagc cgcgataaat aaatatatcc atgcaaaatt tgaggagcat aaaaataaaa
     2461 tagacaactt aaagggtgtg gaacaaaaat tgatggtctt acagtacttt aatgtactga
     2521 atgaacttag taatcaggct atctcttctt tgaatatagg agccttttca gcatcggaag
     2581 tattagctag ggttataata gaacaggctg ccaatcaatt ttatatagca atagatgatg
     2641 gcgaaaatgc acaagcatta ctcaaggcta gtaagaaatt agttcatagt aatggaaaac
     2701 ggtggctgga gtgtttaaaa tccaaaggaa tgactaatcc agcagcaaat gaaaggattc
     2761 gcatgggaaa agaactaact gatctattta atagattgtg gccaaataca cctgaatatc
     2821 caggaaccag aaaacttttt gaattaattg gctgggaaac acactatcat gcccattacg
     2881 ttcccctttg tgattcaata catacattct ctgatgatat ggtaaatata gtatctctat
     2941 ataatgcaat ccaaagtgat gaaactactg ctatcgaatt cattcttgca gttaaacaag
     3001 aaaataaacg gctggcaatt tataattttg taattgctac ggcccttcgg tgtgaggcct
     3061 tagtaaatgt atttaactct ttaggttatc aggatattat aacagaaatg acaccaacaa
     3121 tagatacagt caaccagact ataattcgat atgatgattt tgatcattcc agaattgatg
     3181 attgtcaata attaattgac agaatacgta catgcgcaac aactagtgat agttgactta
     3241 ttttcgctct tgagagacaa ccatactcaa atctcccaca ttgcaggaga tttgaatatg
     3301 aaaacgtcac cgtggaataa agaccgtacc atcggtcaaa aaaggccact tcagatatct
     3361 catatctggg ggatccggat tagacttgag ttggaaggta aaacacgcga tctagctctg
     3421 ttcaacatgg ctttggacag taagctacgg ggctgtgatt tggtcaaact caaagtatca
     3481 gatgctgcat atggcagttc tgtctcaagc agagcaacgg tgttgcaaca gaaaaccggc
     3541 agccctgtcc aatttgagat gcctttaata acacatcttt taaatgttga agaattatcc
     3601 cgcctgctga agaacgttgc tctgagtgtg ataaaccttt ttattcttga tgatgtcagc
     3661 gagaagatgg aatatatccc cgactgggtc gtggttaccc ggttaaacat ctggtggcgc
     3721 taataaatct ggcaagtcac attctgttga atgctgtgac agtgccttac cgagagagcg
     3781 aaattgtggt gttctagtgc atagtcgcca ccattccgga taattctatt gcgttgtttt
     3841 acaagttctt ttacagcgca gatctgtcgc tgacgttgaa tcaatagcct ggatttcaga
     3901 acacaatgcc cgtttgctgg ttggtccggc agtagcatta attcattatc tgaatgaaat
     3961 gtgtcaatcc acagaaaccg ctgtcaatga cgcattttgt cggacgttaa cacgagaaag
     4021 tctggctatt ccgtaatgcc atcttatttc gtaatggatt gagaatgctg gatttttaat
     4081 tatattacgc gcagtattaa tcgcgtaaat gttttgcggt cagcaacggt gggggatcca
     4141 ttgctctgtt caatgaatac catcatacat ttgaatcagg agtcggtatg atgattttgg
     4201 tgacgggagg cgcatggagc gggaagagtc gccatgcaga ggtgcttatt ggggactctt
     4261 cacaggttct gtatatcgct acctcgcaaa tccttgatga tgagatggct gcacggatag
     4321 aacatcatcg gcaaagccgc ccggagcact ggcgcacagt ggagcgctgg caacatcttg
     4381 atgaattaat tcatgcagac attaacccga atgaggctgt gttgctggaa tgcgttacca
     4441 caatggtgac taatctgttg tttgattatg gcggcgataa agaccctgat gaatgggatt
     4501 atcaggcgat ggaacaggcg attaatgctg agattcagtc gttgattgct gcctgccaac
     4561 gttgccccgc aaaggttgta ttagtgacta acgaagtggg aatggggatt gtgccggaga
     4621 gtcgtctggc acgacatttt cgtgatattg ccgggcgggt aaatcagcag ttggccgctg
     4681 cggcaaatga agtatggctg gtggtttcgg gtattggagt aaaaatcaaa tgagcaaatt
     4741 attttgggcg atgctctcat ttattacgcg tctgcctgta ccgcgtcgct ggtcccaggg
     4801 actggatttc gagcattatt ctcgcggtat tattactttt cctttgattg gattattgct
     4861 tggcgcgatt agcgggctgg tcttcatggt gctgcaggca tggtgtggcg caccactggc
     4921 ggcactgttt agcgtacttg tgctggcgct gatgaccggg gaattccatc tggacggcct
     4981 cgctgatacc tgtgatggcg tattttctgc acgtagctgc gatcgcatgt tggaaatcat
     5041 gcgtgatagc cgtttaggca cccacggcgg tctggcattg atttttgtgg tactggcaaa
     5101 gattctggtg ttaagcgagt tggctctgcg tggcgaaccg atccttgcat cgctggcggc
     5161 agcatgtgcg gtcagccgcg ggactgccgc attattgatg tatcgtcatc gttatgcgcg
     5221 ggaagaaggg cttggcaatg tgttcattgg caaaattgat gggcgacaaa cctgcgtcac
     5281 actcggctta gccgctattt ttgctgccgt attattgctg ggtatgcatg gtgtggctgc
     5341 tatggtggtg acgatggtgg cgattttcat cctcggtcag cttctcaaac gtacgctggg
     5401 cgggcaaacg ggcgatacgc tgggagcagc catagagctt ggtgaactgg tcttcctgct
     5461 ggcactgcta taaagagaca tgacatgcaa ataattgccg atttactgaa tacgatccca
     5521 gccatcaatt ctgccgctat gtcgcgtgca caacggcatg ttgacgggtt actcaaacct
     5581 gttggtagcc taggaaagct ggaggcgctt gccatacaac tggcaggaat gccgggggtg
     5641 aatggcatac cgcatgtggg caaaaaagcg gtactggtta tgtgtgccga tcacggcgtc
     5701 tgggaggaag gggtcgctat ttctccaaaa gaagtgacag ccatacaggc tgaaaatatg
     5761 acccgtggaa caaccggcgt gtgtgtgctg gcagcacaag cgggcgctaa cgtccacgta
     5821 attgatgttg gtattgatac tgctgagcct atccccgggc ttatcaacat gcgtgtcgca
     5881 cgaggtagcg gcaatattgc ttcagctccg gcaatgagtc gccgtcaggc tgaaaagttg
     5941 cttttggacg tcatatgtta tacgcgggag ctggcgaaaa acggtgtcac gctgtttggt
     6001 gtaggtgaac tggggatggc aaacacgaca ccggcagcgg caatagtcag cacaatcact
     6061 ggccgggatc ctgaagaagt ggttgggatt ggcgcaaacc tgccgacaga taaactggct
     6121 aataaaattg atgttgtgcg tcgggcgatt acgttgaatc aaccaaatcc tcaggatggt
     6181 gttgatgtcc tggcaaaagt gggtggattt gatttggtcg gaatagctgg agtgatgtta
     6241 ggtgctgctt cctgcggttt acccgtgttg ctggatggat ttctttctta tgctgctgcg
     6301 ctcgcagcct gccagatgtc tcctgcaatc aaaccgtatc tcattccttc tcacttgtcg
     6361 gcagaaaaag gcgcgcgtat agcgctctcg catttggggc tggagcctta tctcaatatg
     6421 gatatgcgtt taggtgaggg gagtggtgca gctctggcga tgtccatcat cgaagctgct
     6481 tgtgcgatat acaacaacat gggcgaactt gctgccagta atattgttct accggggaat
     6541 acgacttctg atttgaacag ctaaaaacga aaagtctgat aaaagctata cttatcggat
     6601 agtattcgcg acttaggaaa gggacatgat gcgtcgtgta aatattcttt gctcatttgc
     6661 tctgcttttt gccagccaaa atagcctggc ggtaacttat ccattacctc cagagggtag
     6721 ccgtttagtg gggcagtcgc ttactgtaac tgttcctgat cacaatactc agccgctgga
     6781 gacttttgcc tcacaatacg ggcaagggtt aagtaatatg ctggaagcga atccgggcgc
     6841 tgatgttttt ttaccgaagc ctggctcaca actcaccatt ccgcagcaac tgattttgcc
     6901 cgccactgtt cgtaaaggga ttgtggttaa cgtcgctgaa atgcgtcttt attactatcc
     6961 gccagacagt aatacagtgg aagtctttcc tattggtatc ggccaggctg ggcgagaaac
     7021 cccgcgtaac tgggtgacta ccgttgaacg taaacaagaa gcaccaacct ggacgccaac
     7081 gccgaacacc cggcgcgaat atgcgaaacg aggggagagt ttgcccgcat ttgttcctgc
     7141 ggggcccgat aaccccatgg ggctgtacgc gatttatatt ggcaggttgt atgccatcca
     7201 cggtaccaat gccaattttg gtattgggct ccgtgtaagt cagggctgta ttcgtctgcg
     7261 taatgacgat atcaaatatc tgtttgataa tgttcctgta ggcactcgtg tacaaattat
     7321 tgatcagcca gtgaaataca caacggaacc ggatggttca aagtggctgg aagttcatga
     7381 gccgctgtcg cgcaatcgtg cagaatatga gtctgaccga aaagtgccat tgccggtaac
     7441 cccatctttg cgggcgttta tcaacgggca agaagttgat gtaaatcgcg caaatgctgc
     7501 gttgcaacat cgatcgggaa tgcctgtgca aattagttct ggttcaagac agatgtttta
     7561 agagcgttgg taattagaaa gtaaaaagcc tgctcgaaag caggcttttt tgaatttggc
     7621 tcctctgact ggactcgaac cagtgacata cggattaaca gtccgccgtt ctaccgactg
     7681 aactacagag gaatcgtgtg aacggggcgc atattaatgg cagtgcatgg tgatgtcaaa
     7741 gggtgaaatg catttttttg tttgtttgct gccaatttca acaaagtggt gagctttcaa
     7801 tcttattggg taatgaacca tcgtggtgca taccctcctt ttatagggca ggtgaatgcg
     7861 actgctgata aaaagggcca atgacgaatc tatcataatc tcttatttga cagttggtta
     7921 gcttgcgcat caacgccaaa atagaactgg caggcatctt gcatactggt tgtaagtaat
     7981 ggcggcactt gggccgattc ttaaaaaccg gaggcaacat gaacttcaga cgcctgaaat
     8041 acttcgtaaa aattgtagat attggtagcc tgacccaggc tgctgaagta ttgcatatcg
     8101 cacaaccagc gctcagccag caggttgcca tactggaagg tgagttaaat caacagcttt
     8161 tgattcgcac aaaacggggc gttacgccaa cagacgccgg aaaaattctc tatacccatg
     8221 cacgggccat tttacgtcag tgtgagcagg cccaactggc ggtgcataac gttggtcagt
     8281 cattatcggg gcaagtctcg attggctttg cgccaggaac cgctgcgtca tccatcacca
     8341 tgcccttatt acaggcggtt cgcgctgaat tcccggaggt cgttatctat cttcatgaaa
     8401 atagtggcgc agtgcttaac gaaaaactga taaatcacca actcgatatg gcggtgattt
     8461 atgagcattc ccctgtggct ggggtatcca gtcaggcttt gctgaaagaa gatcttttcc
     8521 tggtaggaac tcaagattgc ccggggcaaa acgttgatgt taatgctatt gcgcaaatga
     8581 acctttttct ccccagtgat tacagcgctg ttagacttcg tgttgatgag gctttttccc
     8641 tacggcggct cacggcaaaa gtcattggtg aaattgagtc tattgccacg cttaccgcag
     8701 cgattgccag cggcatgggc gttgcagtat tacctgaatc ggctgcgcgt tcgttatgtg
     8761 gcgcagtaaa tgggtggatg tcacgcatta ccactccttc aatgagtctc tctttgtcct
     8821 taaatttacc cgccagagca aacttgtcac cacaggcaca ggcagt
//
LOCUS       DAETPZ010000133         8770 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.134, whole genome shotgun sequence.
ACCESSION   DAETPZ010000133 DAETPZ010000000
VERSION     DAETPZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.134"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(79..870)
                     /gene="aadA1"
                     /locus_tag="K0W59_003689"
     CDS             complement(79..870)
                     /gene="aadA1"
                     /locus_tag="K0W59_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HBI3024548.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(983..1813)
                     /locus_tag="K0W59_003690"
     CDS             complement(983..1813)
                     /locus_tag="K0W59_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HBI3024549.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            2023..3036
                     /gene="intI1"
                     /locus_tag="K0W59_003691"
     CDS             2023..3036
                     /gene="intI1"
                     /locus_tag="K0W59_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HBI3024550.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            3239..3589
                     /locus_tag="K0W59_003692"
     CDS             3239..3589
                     /locus_tag="K0W59_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HBI3024551.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            3715..4275
                     /locus_tag="K0W59_003693"
     CDS             3715..4275
                     /locus_tag="K0W59_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI3024552.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            4278..7244
                     /locus_tag="K0W59_003694"
     CDS             4278..7244
                     /locus_tag="K0W59_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HBI3024553.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            7311..7688
                     /locus_tag="K0W59_003695"
     CDS             7311..7688
                     /locus_tag="K0W59_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI3024554.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7889..8548)
                     /locus_tag="K0W59_003696"
     CDS             complement(7889..8548)
                     /locus_tag="K0W59_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HBI3024555.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1950 a         2479 c         2348 g         1993 t
ORIGIN      
        1 aattatgtgc ttagtgcatc taacgcttga gttaagccgc gccgcgaagc ggcgtcggct
       61 tgaacgaatt gttagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg
      121 aacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata
      181 agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
      241 gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga
      301 caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt
      361 taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc
      421 cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag
      481 atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc
      541 tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac
      601 aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa
      661 aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag
      721 caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac
      781 ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt
      841 cgatacttcg gcgatcacca cttccctcat gatgtttaac gtttgaaata aggggcgccg
      901 agcgccagcg aggggagcca aaagcttgct tttggccgtc ccgacttgat tgaagggttg
      961 ggcgattttg ccattagatt ttttataaat ttagtgtgtt tagaatggtg atcgcatttt
     1021 tcttggcttt tatgcttgat gttaaattcg accccaagtt tcctgtaagt gcggacacaa
     1081 aaacatattt atgtcctgat ttgcttataa taaacccttc aaaccatccg ttttgtaagg
     1141 ttctatttgc tgtgaatcct gcaccagttt tcccatacag ttttgtacta ttatccagat
     1201 cttgtagata catgttctct atggtgtttt ctatggctga gtttttaact gggagattgt
     1261 gattaataat tttacgcagg aattgaattt gttcttctgg tgaaattttt aagctacttt
     1321 cgagccatgc ttctgttaat ccgttgtttc tttctttatc tccagagaag tcttgatttc
     1381 cataatcaaa atctttgaga taattcttga ttttatttaa tccaattttt tgggttattt
     1441 cttgcgaaac ccaaacaaca gaaaattgca tccacgtctt tggtgtatga ttgctgttcc
     1501 agatctccat tcctttgggg gttttatccc atttgaatat ggttttctga tctattattt
     1561 ccgcatcaaa tgccataagt gataatgcga tcttgaaagt tgaatctggt gccatttgcg
     1621 ttgcacactt tgctttattg aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta
     1681 aaaaacaacc ttcagttcct tcaaataatg gagatgcaac agtagagata tctgttgatg
     1741 cactggcgct gctgtagata atatttgcaa ttattaaaaa aatagcgaag ttgatatgta
     1801 ttgtgttttt cataataagt attggtttgg taaagggctt aattttaacg gctaacaatt
     1861 aatgaggctc cgggttcgcc caactttgtt ttagggcgac tgccctgctg cgtaacatcg
     1921 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
     1981 cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
     2041 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac
     2101 ttgcattaca gcttacgaac cgaacaggct tatgtccact gggttcgtgc cttcatccgt
     2161 ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc
     2221 tggctggcga acgagcgcaa ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg
     2281 ctgttcttct acggcaaggt gctgtgcacg gatctgccct ggcttcagga gatcggaaga
     2341 cctcggccgt cgcggcgctt gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc
     2401 ggttttctgg aaggcgagca tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg
     2461 atcagtgagg gtttgcaact gcgggtcaag gatctggatt tcgatcacgg cacgatcatc
     2521 gtgcgggagg gcaagggctc caaggatcgg gccttgatgt tacccgagag cttggcaccc
     2581 agcctgcgcg agcagctgtc gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc
     2641 cgcagcggcg ttgcgcttcc cgacgccctt gagcggaagt atccgcgcgc cgggcattcc
     2701 tggccgtggt tctgggtttt tgcgcagcac acgcattcga ccgatccacg gagcggtgtc
     2761 gtgcgtcgcc atcacatgta tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa
     2821 caagcaggca tcacgaagcc cgccacaccg cacaccctcc gccactcgtt cgcgacggcc
     2881 ttgctccgca gcggttacga cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc
     2941 tctacgacga tgatttacac gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg
     3001 cttgatgcgc tgccgcccct cactagtgag aggtagggca gcgcaagtca atcctggcgg
     3061 attcactacc cctgcgcgaa ggccatcggt gccgcatcga acggccggtt gcggaaagtc
     3121 ctccctgcgt ccgctgatgg ccggcagcag cccgtcgttg cctgatggat ccaacccctc
     3181 cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacaat
     3241 ggaggtggta gccgagggtg tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg
     3301 ttgcgacacg gtgcagggtt tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg
     3361 cttcgtcaac caatggagga acaccaccat gaacgccaat gaaccgagca ccagttgctg
     3421 cgtgtgctgc aaggaaatcc cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt
     3481 ggagcatttc tgcgggctgg agtgctatca gcgcttccag gcgcgggcca gcactgcgac
     3541 cgaaaccagc gtcaaaccgg acgcttgtga ttcgccgccg tcaggttgag gcatacccta
     3601 acctgatgtc agatgccatg tgtaaattgc gtcaggatag gattgaattt tgaatttatt
     3661 gacatatctc gttgaaggtc atagagtctt ccctgacatt ttgcagggaa ttccatgact
     3721 ggacagcgca ttgggtatat cagggtcagc accttcgacc agaacccgga acggcaactg
     3781 gaaggcgtca aggttgatcg cgcttttagc gacaaggcat ccggcaagga tgtcaagcgt
     3841 ccgcaactgg aagcgctgat aagcttcgcc cgcaccggcg acaccgtggt ggtgcatagc
     3901 atggatcgcc tggcgcgcaa tctcgatgat ttgcgccgga tcgtgcaaac gctgacacaa
     3961 cgcggcgtgc atatcgaatt cgtcaaggaa cacctcagtt ttactggcga agactctccg
     4021 atggcgaacc tgatgctctc ggtgatgggc gcgttcgccg agttcgagcg cgccctgatc
     4081 cgcgagcgtc agcgcgaggg tattgcgctc gccaagcaac gcggggctta ccgtggcagg
     4141 aagaaatccc tgtcgtctga gcgtattgcc gaactgcgcc aacgtgtcga ggctggcgag
     4201 caaaagacca agcttgctcg tgaattcgga atcagtcgcg aaaccctgta tcaatacttg
     4261 agaacggatc agtaaatatg ccacgtcgtt ccatcctgtc cgccgccgag cgggaaagcc
     4321 tgctggcgtt gccggactcc aaggacgacc tgatccgaca ttacacattc aacgataccg
     4381 acctctcgat catccgacag cggcgcgggc cagccaatcg gctgggcttc gcggtgcagc
     4441 tctgttacct gcgctttccc ggcgtcatcc tgggcgtcga tgaactaccg ttcccgccct
     4501 tgttgaagct ggtcgccgac cagctcaagg tcggcgtcga aagctggaac gagtacggcc
     4561 agcgggagca gacccggcgc gagcacctga gcgagctgca aaccgtgttc ggtttccggc
     4621 ccttcaccat gagccattac cggcaggccg tccagatgct gaccgagctg gcgatgcaaa
     4681 ccgacaaagg catcgtgctg gccagcgcct tgatcgggca cctgcggcgg cagtcggtca
     4741 ttctgcccgc cctcaacgcc gtcgagcggg cgagtgccga ggcgatcacc cgtgctaacc
     4801 ggcgcatcta cgacgccttg gccgaaccac tggcggacgc gcatcgccgc cgcctcgacg
     4861 atctgctcaa gcgccgggac aacggcaaga cgacctggtt ggcttggttg cgccagtctc
     4921 cggccaagcc aaattcgcgg catatgctgg aacacatcga acgcctcaag gcatggcagg
     4981 cactcgatct gcctaccggc atcgagcggc tggttcacca gaaccgcctg ctcaagattg
     5041 cccgcgaggg cggccagatg acacccgccg acctggccaa attcgagccg caacggcgct
     5101 acgccactct cgtggcgctg gccaccgagg gcatggccac cgtcaccgac gaaatcatcg
     5161 acctgcacga ccgcatcctg ggtaagctgt ttaacgctgc caagaataag catcagcagc
     5221 agttccaggc gtcaggcaag gccatcaacg ccaaggtacg tctgtacggg cgcatcggtc
     5281 aggcgctgat cgacgccaag caatcaggcc gcgatgcgtt tgccgccatc gaggccgtca
     5341 tgtcctggga ttcctttgcc gagagcgtca ccgaggcgca gaagctcgcg caacccgatg
     5401 acttcgattt cctgcatcgc atcggcgaga gctacgccac cctgcgccgc tatgcaccgg
     5461 aattccttgc cgtgctcaag ctgcgggccg cgcccgccgc caaaaacgtg cttgatgcca
     5521 ttgaggtgct gcgcggcatg aacaccgaca acgcccgcaa gctgccagcc gatgcaccga
     5581 ccggcttcat caagccgcgc tggcagaaac tggtgatgac cgacgccggc atcgaccggc
     5641 gctactacga actgtgcgcg ctgtccgagt tgaagaactc cctgcgctcg ggcgacatct
     5701 gggtgcaggg ttcacgccag ttcaaggact tcgaggacta cctggtaccg cccgagaagt
     5761 tcaccagcct caagcagtcc agcgaattgc cgctggccgt ggccaccgac tgcgaacaat
     5821 atctgcatga gcggctgacg ctgctggaag cacaacttgc caccgtcaac cgcatggcgg
     5881 cagccaacga cctgccggat gccatcatca ccgagtcggg cttgaagatc acgccgctgg
     5941 atgcggcggt gcccgacacc gcgcaggcgc tgatagacca gacagccatg gtcctgccgc
     6001 acgtcaagat caccgaactg ctgctcgaag tcgatgagtg gacgggcttc acccggcact
     6061 tcacgcactt gaaatcgggc gatctggcca aggacaagaa cctgttgttg accacgatcc
     6121 tggccgacgc gatcaacctg ggcctgacca agatggccga gtcctgcccc ggcacgacct
     6181 acgcgaagct cgcttggctg caagcctggc atacccgcga cgaaacgtac tcgacagcgt
     6241 tggctgaact ggtcaacgct cagtttcggc atccctttgc cgggcactgg ggcgatggca
     6301 ccacatcatc atcggacgga cagaatttcc gaaccgctag caaggcaaag agcacggggc
     6361 acatcaaccc aaaatatggc agcagcccag gacggacttt ctacacccac atctccgacc
     6421 aatacgcgcc attccacacc aaggtggtca atgtcggcct gcgcgactca acctacgtgc
     6481 tcgacggcct gctgtaccac gaatccgacc tgcggatcga ggagcactac accgacacgg
     6541 cgggcttcac cgatcacgtc ttcgccctga tgcacctctt gggcttccgc ttcgcgccgc
     6601 gcatccgcga cctgggcgac accaagctct acatcccgaa gggcgatgcc gcctatgacg
     6661 cgctcaagcc gatgatcggc ggcacgctca acatcaagca cgtccgcgcc cattgggacg
     6721 aaatcctgcg gctggccacc tcgatcaagc agggcacggt gacggcctcg ctgatgctca
     6781 ggaaactcgg cagctacccg cgccagaacg gcttggccgt cgcgctgcgc gagttgggcc
     6841 gcatcgagcg cacgctgttc atcctcgact ggctgcaaag cgtcgagcta cgccgccgcg
     6901 tgcatgccgg gctgaacaag ggcgaggcgc gcaatgcgct ggcccgtgcc gtgttcttca
     6961 accgccttgg tgaaatccgt gaccgcagtt tcgagcagca gcgctaccgg gccagcggcc
     7021 tcaacctggt gacggcggcc atcgtgctgt ggaacacggt ctacctggag cgtgcggcgc
     7081 atgcgttgcg cggcaatggt catgccgtcg atgactcgct attgcagtac ctgtcgccac
     7141 tcggctggga gcacatcaac ctgaccggtg attacctatg gcgcagcagc gccaagatcg
     7201 gcgcggggaa gttcaggccg ctacggcctc tgcaaccggc ttagcgtgct ttattttccg
     7261 ttttctgaga cgacccctat tatggcgaag gtatcattca ggaagcgttg atgaaggatt
     7321 atctctgtaa aaagttattc aaccggcttt ccggtaccct ggtgatcaga gcgcgctgcg
     7381 gcaataacat tactggcctg gcatgttgca atattcttta tccctcgccc cgatacagcg
     7441 gtcagctgca tattaaagag ctgtatgttt ctcagtgcga tcgaaataag ggcacgggta
     7501 aagcgataat gcgctttata gcccggcttg cgcttgaaca ggaatgcctt agccttagct
     7561 ggaacgctga aaaatccaac cccggcgcta accgttttta tcaggctctg ggaggcagaa
     7621 taaatgatca tatcgtcaat tattacctcc acggggagag cctgagcaaa ctggcctcag
     7681 gcatttgaga agcacacggt cacactgctt ccggtagtca ataaaccggt aaaccagcaa
     7741 tagacataag cggctattta acgaccctgc cctgaaccga cgaccgggtc gaatttgctt
     7801 tcgaatttct gccattcatc cgcttattat cacttattca ggcgtagcaa ccaggcgttt
     7861 aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca tcgcagtact
     7921 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct
     7981 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
     8041 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
     8101 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
     8161 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
     8221 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
     8281 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg aattccggat
     8341 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
     8401 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
     8461 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
     8521 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
     8581 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
     8641 cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag
     8701 ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcggcgc
     8761 aaagtgcgtc
//
LOCUS       DAETPZ010000134         8716 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.135, whole genome shotgun sequence.
ACCESSION   DAETPZ010000134 DAETPZ010000000
VERSION     DAETPZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.135"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(121..447)
                     /locus_tag="K0W59_003697"
                     /pseudo
     CDS             complement(121..447)
                     /locus_tag="K0W59_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(496..924)
                     /locus_tag="K0W59_003698"
                     /pseudo
     CDS             complement(496..924)
                     /locus_tag="K0W59_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            complement(924..1631)
                     /locus_tag="K0W59_003699"
     CDS             complement(924..1631)
                     /locus_tag="K0W59_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBI3024556.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1646..2323)
                     /gene="ydjY"
                     /locus_tag="K0W59_003700"
     CDS             complement(1646..2323)
                     /gene="ydjY"
                     /locus_tag="K0W59_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HBI3024557.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(2328..3038)
                     /locus_tag="K0W59_003701"
     CDS             complement(2328..3038)
                     /locus_tag="K0W59_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBI3024558.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYNLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(3205..4011)
                     /gene="xthA"
                     /locus_tag="K0W59_003702"
     CDS             complement(3205..4011)
                     /gene="xthA"
                     /locus_tag="K0W59_003702"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBI3024559.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGY
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            4457..5035
                     /locus_tag="K0W59_003703"
     CDS             4457..5035
                     /locus_tag="K0W59_003703"
                     /inference="COORDINATES: protein motif:HMM:NF012428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBI3024560.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGSIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDATCAVIVEPI"
     gene            5051..5677
                     /locus_tag="K0W59_003704"
     CDS             5051..5677
                     /locus_tag="K0W59_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBI3024561.1"
                     /translation="MPASNAFLQGLRELCDRHNALLIFDEVQTGVGRTGELYAYMHYG
                     VTPDLLTTAKALGGGFPVGALLATEECARVMTVGTHGTTYGGNPLASAVAGKVLELIN
                     TPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRGLGLLIGCVLNADYAGQAKQISLEA
                     AKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDRFAAACEHFVSRGSS"
     gene            5674..6507
                     /gene="astA"
                     /locus_tag="K0W59_003705"
                     /pseudo
     CDS             5674..6507
                     /gene="astA"
                     /locus_tag="K0W59_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
     gene            6503..7432
                     /locus_tag="K0W59_003706"
                     /pseudo
     CDS             6503..7432
                     /locus_tag="K0W59_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            7429..>8716
                     /gene="astB"
                     /locus_tag="K0W59_003707"
     CDS             7429..>8716
                     /gene="astB"
                     /locus_tag="K0W59_003707"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HBI3024562.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFKDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMLLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQSVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQ"
BASE COUNT         2040 a         2238 c         2392 g         2046 t
ORIGIN      
        1 aacttgtgaa attaccgacg agaaggggaa gggccttccc gagagtggta aaggagaggc
       61 cctggaaata ccccacagcc ccgaaaaaga gccagtcagt tgcttaactg actggcatta
      121 ctcttttaag gtgcggaagt tttcgccgtt cacccagagc agagccaccg agccgcccgt
      181 tttacgaccg gcagcagctt cggtctgaat gcgcttcacc gcgtctgcgg catccgcgag
      241 acgaacaatc ttcaggttta tagcgtaatg ggttttcatc tcgccgctaa cccagtcgag
      301 atagcggtta attgcggtat cgccgcccca ggcgttaaac cagacggttt gccctttggc
      361 ctcattttta atttcctgcc agtcactggc atgtgttgcc aaagaaaaca gcgataacaa
      421 tcccagcaac cacccacaat ggcgcatctt ccctccttaa gaacgttttt ccggaatacg
      481 gcacagcccc tgagcgtcaa aatgcgcagc cagtcgggta cgcagggcat catcgactgt
      541 tggcggtgtg acgcttgcgg cttcggtgta ctccagcgcc agccgttctt catcgctaaa
      601 gagcgggctt tgccgccagt cagccactgc cagcagttta tcggtgctgc cggtacgctc
      661 ggcgactttc atactggtga tatccacgca aaactcacac aggcacattt gcgcaatgcg
      721 cgcgctgaca agcgatcgca ctaccggatc gagcggtgag cgtttgcgct ccagccaccc
      781 aacaaacatt gacaccaaat aaaagataaa cgggatccgc ccccaccagc gaatcgggct
      841 tagcacctcg ccgtaatggc gacgatgcag ccacgcctgc ggacgtaaaa tgaaaggagt
      901 tttgctaagc ggcggtaaac ccatcagtca ttcctcttct ggcgctcaag ccatattttc
      961 ttcgccataa aaatcaccac ggtcagggca aacagaataa acagttcggt gacaaaccag
     1021 aaagtaccgc ctgttaacat gctgcccgcc caggaataaa caatagtcgc cggtaattga
     1081 ccaagcccgg tggcgataaa aaacgagcga aaacgtattg aagtcagacc ggcagcatag
     1141 ctgattggat cgaaagggac aaaaggcaat aaccgacaga ccaggatggt gtgtttgccg
     1201 tagcgagtga aaaagccgtc catactgtca agcacggttt tgccggttaa tttttccacc
     1261 acttcgcggc ccatcactct ggcgataaaa aagcacagcg ccgcgccggc catcgaactg
     1321 gtccacgaca gcaagccgcc ccagaacgcg ccaaacagcg acgcattggc aaaggtgatc
     1381 aaaaacgcag gtagcggtgc agcaatggcc tgtaaaatca tcaacaagaa cgagacaact
     1441 gccgccagtg cgccgtaaga ctgaataaag cgttctatac cctgttggtc cacggcggca
     1501 aacgccgcaa cgctgcgatt gataaactca tggacgccgg gaagcagcgc ccatgcgagc
     1561 agcatagcga acaacaggat gatgagggtt atacggtagt accagatttt acgcgattgt
     1621 atcttcatca tcctttatcc tggctttatt cggtgatttt aaaggtaacc gttgccagcg
     1681 tgttatccgc cgggagaact gaggcattgc ctttgaattt cacttcgccg cgtttttcca
     1741 ccgcaccata agtgtatgtt gcattgctga cgatgccgac cgggcagcta tccagacaca
     1801 ccaggcaacc cgttttcttc tcttctgctg ccgttaaatt accgccaaag cgcatgtcca
     1861 gtttcttgcc attactgtca acaatcactt catcgaagga atacgctttt gccgcccctt
     1921 gccagttgac cgaaatatcc agtttgctgc ctgtgacatg agtcgtttct ttattatcca
     1981 tcgtcatgtt ttcgcccggc gtaccacctg cctctttcag ggcttcataa aatgctttcg
     2041 gggtcgcata agccataaac agcgatttat gtccgttgga gccatcttta aacacaatac
     2101 cgtgacgagt gtcgtcggtg aggaaacgac cattaatttg caccagcatg gttaccgtat
     2161 ggttattggc atcgacgcgc agcggttggc tgttcgaaag tccgtcgtat tccacttttg
     2221 ccgccgcatt ctctttttga tcgcagccgc taagcccggc aacagccagt aagcagagtg
     2281 cagccagtcc ttttttccat gacactgaat aatgttgcga catcgttcta tccttcattt
     2341 tttgggtgag taagtgggct gcggcgcgaa gcagacagat ccacatgttt gtgtcgggcg
     2401 tagagttttg cgagctggat gagaataaac agcgccaggc ccgccagaca gagttgtaaa
     2461 ataaagcgca gcgtaatgcc ttcattggcg agatcgcttg ccatcacggt ataaataaca
     2521 ataccgggca gggtcgtgag tgccgaaata agggtataag gccagaaggc gatggtggtt
     2581 aatccgtaag cgtaattttg aatattgtaa ggaaacaacg ggattaagcg ggtcagaata
     2641 agaaaatcaa taccgttacg cgcaatgcct ttttcaatgg cctggaaggt atggctatgg
     2701 cccacgtatt tcagcagtaa atcacgtccc agccagcgcg ccagcaggaa tgagcacgag
     2761 gaggccagcg ttgcggcaat taatgagagt agtgtcccta agagcgggcc aaaaactatt
     2821 cctccagcga tcaccagtat gcttcctggt aacagaaaga ggggggcatt gatgaataac
     2881 agaatataga ggttataacc gaacaatccg ctctggcgaa tgagtgtctg taagtggggt
     2941 aaatcggtga gcagatcgaa taaaccgaaa gcgtggatag cgtaaatgac cagcgcaaaa
     3001 ataagacagg caaacagaaa gttacgctca gcgttcatcg tggagtttcc tatatcaaac
     3061 ttgataatag agaagtggtc agacaactaa aaacgcagtg acggtataac aaaggacggc
     3121 aggcaacaaa tcattactga cagtttgttg atttttctca aggttaattc tcctgaccca
     3181 gtttgagcca ggagagctgc taaattagcg gcggaaggtc gcccagacgg gggcgtgatc
     3241 ggacggtttt tccatgctgc gaatttcata gtcgatgccg gtttctacgc aacattctgc
     3301 cagcggttgg ctggcgagca gcaggtcgat gcgcagacca cggttatcgt caaaaccttt
     3361 tgagcggtaa tcaaaccatg agaaacgatc tgctgtttgc ggattagcat ggcggaaggt
     3421 atcgaccacc ccccagctca tcagcctgtc catccattcg cgctcttccg gcaggaaaga
     3481 gcatttaccg gtacgcagcc agcgcttacg gttttcttcg ccaatgccga tatccagatc
     3541 tgtagggctg atattcatat cgcccataat cagtaccgga ttatcacgtt tgagttcggt
     3601 ttccaggtag ttttgcagat tctgataaaa ctgcgctttt gccgggaatt ttatcggatg
     3661 gtcgcggctt tcaccctgcg ggaagtaacc gttgatcacg gtgacataac ccagcagtga
     3721 ggggatttcc gccataataa tccgccgctg cgcctcttcg tcgtcaccgg gaaagccgcg
     3781 acgcacggca atcggcgtct ctttggtcag cagcgccacg ccataatggc ctttctgccc
     3841 gtgataaaac acgttgtagc cgagcttcgc cacctcttcg agcggaaaca tatcgtcatg
     3901 aacttttgtc tcctgcaggc caatcacatc cggttggtgc ttttcgacga tggcttcaag
     3961 ctggtgaggt ctggcgcgca ggccgttgat attaaaagag acaaatttca tagtcgctgc
     4021 catttattca gataaagagt gcgtggatgg tagcagaatt tcgcgttgct taccgcctgt
     4081 tgttaagtga tttgatcgat ggttgttaat gatgtcaacg atggcgcaaa aaatgcccgc
     4141 ttttggtgcg cgctgcgtca gaatggcgca gtaatttcca gtaaatttcg tcaatgatca
     4201 caattccaga attatatttt acttctgcat aacattgcgt tttttattct ttttattcac
     4261 ccacatcgca aacgtattca ctttatatgc actttaaatg catatggttt ggttataact
     4321 ccttgatttc cagttagcct ccgccgttta tgcactttta tcactggctg acacgaaccc
     4381 tgcaatctac atttacagcg caaacattac ttattgttaa catacaaata acgaattatt
     4441 tactgtagag gtcgctatgt ctcagccaat tacgcgtgaa aactttgatg aatggatgat
     4501 acctgtttac gctccggcac cctttatacc ggtacgtggc gaaggttcgc gcttgtggga
     4561 tcagcagggg aaagagtata tcgacttcgc gggtagcatt gcggtgaacg cgctgggcca
     4621 tgcgcatccg gaactgcgtg aagcgctgaa cgaacaggcg agtaagttct ggcataccgg
     4681 caacggttac accaacgagc cggtactgcg actggcgaaa aaattgatcg acgccacgtt
     4741 tgccgatcgc gtcttctttt gtaactccgg tgcggaagcc aacgaagcgg cgctaaaact
     4801 ggcgcgtaaa ttcgctcacg accgctacgg cagccataag agtggcatcg tggcgttcaa
     4861 aaatgcgttt catggtcgca cgctgtttac tgtcagtgcg ggtgggcagc cagcctattc
     4921 acaggatttt gcgccactgc cggcggatat tcgtcatgct gcatataacg atattaactc
     4981 tgccagcgcg ctgattgacg acgccacctg tgcggtgatt gtcgagccca tctagggcga
     5041 aggcggtgtg gtgccagcca gcaacgcgtt tttacaaggt ctgcgtgaat tgtgtgaccg
     5101 ccacaatgcc ttgctgattt ttgatgaggt acaaaccggc gtcgggcgca ctggtgagct
     5161 gtatgcctat atgcactacg gcgtgacgcc cgatctgtta accaccgcca aagcgctggg
     5221 cggcggtttc ccggtcggtg cgttgttggc aaccgaagag tgcgcccgcg tgatgaccgt
     5281 tggcactcat ggcaccacct atggcggtaa tccgctggcc tcggcggtag caggcaaagt
     5341 gctggagctc atcaacacac cagagatgct taatggcgtt aaacagcgtc acgactggtt
     5401 tgttgagcgt cttaatacta ttaatcaccg ctatggtttg ttcagtgaag ttcgcggctt
     5461 aggtttgttg attggctgtg tactgaatgc cgattacgcc gggcaagcga aacagatctc
     5521 tctggaagcg gcgaaagcag gcgtgatggt actgattgcg ggtggcaacg tggtgcgttt
     5581 tgcgcctgcg ctcaatgtca gcgaagaaga ggtgacgacc ggactggatc gctttgcagc
     5641 tgcttgcgaa cactttgtta gccgaggttc atcatgatgg tcatccgtcc cgttgagcgc
     5701 tcagatgtct cggcgctgat gcagcttgcc agcaaaacgg gcggcggcct gacgtcgctt
     5761 cccgccaatg aagccacgct ttcggcgcgt atcgaaaggg caatcaaaac ctggcaaggc
     5821 gaactgccta aaagcgagca gggctatgtg ttcgtgctgg aagatagcga gacaggcacc
     5881 gtggcgggga tttgcgctat tgaggtggcg gttgggctga acgatccctg gtacaactat
     5941 cgcgtcggca cgttggttca cgcctcaaaa gagctgaatg tctataacgc attgccgacg
     6001 ctgtttctta gtaacgatca caccggcagc agcgagctgt gcacgctgtt tctcgacccg
     6061 gactggcgca aagagggcaa cggctatttg ctgtcgaaat cgcgctttat gtttatggcg
     6121 gcttttcgcg acaagtttaa tgacaaagtg gttgctgaaa tgcgcggggt gattgacgaa
     6181 cacggctatt caccgttctg gcaaagcctc ggtaaacgct tcttttcgat ggattttagc
     6241 cgcgccgatt ttctctgcgg caccgggcaa aaggcattta ttgcagaact gatgccgaaa
     6301 catccgatct atacccactt tttatcccag gaagcccagg acgtcatcgg tcaggtacat
     6361 ccgcaaaccg cgcctgcccg cgcggtgctg gagaaagaag gttttcgtta ccgcaactat
     6421 atcgacatct tcgacggcgg gccaacgctt gagtgtgaca tcgaccgcgt gcgcgccatc
     6481 cgtaaaagtc ggctggtgga agtggcagca ggctggcttg ccgccgggcg tgctgaacct
     6541 ggtgcagggc gggcgtgaaa cgggtcaggc gctgagtgcg ctggaggatc tcgacggttt
     6601 gctgtttacc ggtagcgcca ataccggcta ccagctgcat cgccagctct ccggtcagcc
     6661 ggagaaaatt ctcgcccttg agatgggcgg taataatccg ctaattatcg atgaggtggc
     6721 ggatatcgac gcggctgtcc atctgaccat tcagtcggcg tttgtcacag ccgggcaacg
     6781 ctgcacctgc gcccgccgtt tattgctcaa aagcggagcg cagggcgatg cgtttcttgc
     6841 tcgtctggtt gccgtcagcc agcgattaac gccgggcaac tgggatgacg aaccgcagcc
     6901 gtttattggc gggctgattt ctgaacaggc cgcacagcag gtggttactg cctggcagca
     6961 actggaagcg atgggcggac gaaccctgct tgcgccgcgc ttattacaag cagggacatc
     7021 gttgctgaca ccagggatca ttgaaatgac gggcgttggc ggtgtgccgg atgaagaggt
     7081 gttcgggccg cttttgcgcg tctggcgtta tgatactttt gatgaggcga ttcggatggc
     7141 gaacaacact cgcttcggac tcttttgcgg tctggtttcc cccgagcggg aaaaattcga
     7201 tcaactgttg ctggaggcgc gggcggggat tgttaactgg aacaaaccgc ttactggtgc
     7261 tgccagtacc gcgccattcg gcggcattgg tgcttccggt aaccatcgcc ccagcgcctg
     7321 gtatgccgca gattactgcg catggcctat ggcgagcctg gagtcggact cgttaacatt
     7381 gcccgccacg cttaaccccg ggctggattt ttccgatgag gtggtgcgat gaacgcctgg
     7441 gaagttaatt tcgacgggct ggtagggctg acgcatcatt acgcgggcct gtcgtttggt
     7501 aatgaagcct ctacccgtca ccgttttcag gtgtctaacc cgcgactggc ggcaaaacag
     7561 ggcttactga aaatgaaagc ccttgccgat gcgggattcc cccaggccgt gatcccgccg
     7621 cacgagcgtc cgtttattcc ggtgctgcgt cagttgggat tcagtggtag cgatgagcag
     7681 gtactggaaa aagttgcacg ccaggcaccg cactggcttt ccagcgtcag ttccgcttcg
     7741 ccaatgtggg tagccaatgc ggcaacgatc gcgccatctg ccgatacgct ggatggcaaa
     7801 gtgcatctca ccgttgccaa cctgaacaat aaatttcacc gttcgctgga agcgcccgtc
     7861 actgaatcgc tgttaaaagc gatttttaag gacgaagaga aatttagcgt ccattcggcg
     7921 ttgccgcagg tagcgttgct cggtgatgag ggggcggcaa accacaatcg tctcggcggt
     7981 cattacggtg aaccgggtat gctacttttt gtctacgggc gagaagaagg caatgatacc
     8041 cggccttccc gttatccggc gcgacagact cgcgaagcca gcgaggcggt ggcaaggctt
     8101 aatcaggtga atccccaaca ggtgattttc gcccagcaaa acccggacgt tatcgaccag
     8161 agcgtttttc ataatgacgt gattgccgtg agtaaccgcc aggtgctgtt ttgccaccaa
     8221 caggcgttcg ctcgccagtc acagttactg gcaaacctgc gggcgcgggt gaacggtttt
     8281 atggcgatag aagttccggc aactcaggtt tccgtgtcgg atgcggtgtc tacctatctg
     8341 tttaacagcc aactgttgag ccgcgatgat ggttccatga tgttggtgct gcctcaggag
     8401 tgtcgggaac acgccggagt atggggttat ctcaatgaac tccttgccgc tgacaacccg
     8461 attagcgaac taaaagtctt tgacttacgt gaaagcatgg cgaatggcgg cggcccggcg
     8521 tgcctgcggt tgcgggtggt attgacagaa gaagaacgcc gggcggtgaa tccggcggtg
     8581 atgatgaacg ataccttgtt taatgcgctc aatgactggg tggatcgtta ctaccgcgat
     8641 cgtcttactg ctgccgatct ggccgacccg caattgctgc gcgaagggcg ggaagcactg
     8701 gatgtattga gccaat
//
LOCUS       DAETPZ010000135         8393 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.136, whole genome shotgun sequence.
ACCESSION   DAETPZ010000135 DAETPZ010000000
VERSION     DAETPZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.136"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(90..281)
                     /locus_tag="K0W59_003708"
                     /pseudo
     CDS             complement(90..281)
                     /locus_tag="K0W59_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            279..603
                     /locus_tag="K0W59_003709"
                     /pseudo
     CDS             279..603
                     /locus_tag="K0W59_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(633..3545)
                     /locus_tag="K0W59_003710"
     CDS             complement(633..3545)
                     /locus_tag="K0W59_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024563.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGSIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGAAPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3548..3664
                     /locus_tag="K0W59_003711"
     CDS             3548..3664
                     /locus_tag="K0W59_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBI3024564.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            4028..4870
                     /locus_tag="K0W59_003712"
     CDS             4028..4870
                     /locus_tag="K0W59_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBI3024565.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGRECLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4873..5961
                     /gene="capU"
                     /locus_tag="K0W59_003713"
     CDS             4873..5961
                     /gene="capU"
                     /locus_tag="K0W59_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HBI3024566.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPTNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5966..6916
                     /gene="virK"
                     /locus_tag="K0W59_003714"
     CDS             5966..6916
                     /gene="virK"
                     /locus_tag="K0W59_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HBI3024567.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNQIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6981..7925
                     /gene="lpxM"
                     /locus_tag="K0W59_003715"
                     /gene_synonym="msbB"
     CDS             6981..7925
                     /gene="lpxM"
                     /locus_tag="K0W59_003715"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBI3024568.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(8079..>8393)
                     /locus_tag="K0W59_003716"
     CDS             complement(8079..>8393)
                     /locus_tag="K0W59_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3024569.1"
                     /translation="ISLPKRLKATYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRG
                     IYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLA
                     KV"
BASE COUNT         2234 a         1895 c         2006 g         2258 t
ORIGIN      
        1 ggccttcccg agagtggtaa aggagaggcc ctggaaatac cccacagccc cgaaaaagag
       61 ccagtcagtt gcttaactga ctggcattaa ggccgccggg gacgtttttt taccaccggg
      121 agtttgggca taacggtgct ttcagtaagt acgacaggta ctgtggaggc acttccgtaa
      181 agaagggcat caagctcctg ctccgacata cctgcaggca aaggccatga aagccctgat
      241 ttccggaagc gagcgaacag tccacaaact gttgaacgtg gtgtcagcgg cggacaggga
      301 taagccctcg tcagcagttc cgtcagcact gcgacagcgt tctttacccg gtcaaaacag
      361 cgttgcggtg caccacgcct gacggatgaa ctgcgtgctc agggttacca ctttaacgta
      421 aaaaccgtgg cggcaagcct gcgccgtcag gggctgaggg caaaggcctc ccggaagttc
      481 agcccggtca gttaccgcgc acatggcctg cccgtgtcag aaaatctgct ggagcaggat
      541 ttttacgcca gtggtccgaa ccagaagtgg gcgggagaca tcacgtactt acgtacagat
      601 gaagttcggt tacatcctgt cagcacggaa cctcacgctt tttgagcggc acgacggatc
      661 ccccccaccc agtatccgac agacttttca ctgcgttcgt aaaaaccctt cttccctccc
      721 agcatatcca ctaattccgg tgacatatcc tgcagataag tctgcaaatc atgccccaga
      781 tcttccggtg actgcaactc ataaaccgcg cgccacacat caccctgcct tattttaagc
      841 cccgggttaa gaatatactg tccgtggttc agcctcagaa aaagcttttt tccataagga
      901 tttttgctgt taacttcatg ttgagagagc tggctgctga tataactcct tcgtttgcgg
      961 cgttccggca gcaatgcttc aggaagatga accacacact ctgccagtcg tgccgcactg
     1021 aaggcttccg aaaaaaacag agcacttccc agatgcgtgt ggaacagcgc ctgcatcagg
     1081 atcaccagaa acattgccat ctgccggttg tccagtttga tgaggcgtcc ttccacctgc
     1141 agtgacagac tttcaggttt cagcagggta tacaactgcg cacttttctg ttgcgcataa
     1201 cggggcgtac tgaccgccgc tgacagaact tgatgataag ccgccagccc gtcattaccg
     1261 gtcagcaagg gagctgcacc acgctccagc agtaactgaa ccagtgcaat attacctgcc
     1321 cgggctgctg acatcagcgg agtaagatta aacggcgaac ggtgatcaac accataacgt
     1381 tcaatatccc gcaacacggc aaccgggtta ttcattgagt atggccggaa atacttcagc
     1441 tccagtaact gcagagtctt cgttctgggc tgccttgtgg gcttaaaatc ttcccggtac
     1501 agtgcattat acagcgggaa cagggaataa atcagactgt actccagcgc ctgcagttgt
     1561 atggttttat cggctgtacc tgcgaggata tgctgtttcc actgtcgtgc atcctctgcg
     1621 gtgataatct gccatggcat ctcagatgtc tgcaggatcc ggctacggat ggtgtccgcc
     1681 tgctcctgct tcccctgttt ttcaagcaga tgtgcggttt tctgccactc atcacggctg
     1741 gaaatctctt cctgcagatt cagaggctct tcctgatgcg tgagcgcaag cagactccac
     1801 agaggatgct ccacctgctg ttcaacaaga tacacattgt gtgtagcacg ggtgagcgca
     1861 acatacagcg cgttggtaaa aaatttatag atttccgctg aacgatcctg cctgtcccgc
     1921 ggacgggaat aacgggcctc gccctcaaga tcagccggtg taagaccctc gcaaatatca
     1981 tcaaatgcct gccgggcagc gcttactata ttgtaaagga tcaccgtttc gtattccagc
     2041 cccttaacct cctgaaccga aaagaccagc ggcgtactga accagcatcg cgcctgtgct
     2101 ttctgttccg gatgcatcac aatgaccgct acacgattcg aaagtgaggt tttggcattc
     2161 agttcctgct ttgtctcttc cctgtcgtcc agcaaccgga tgctcccttc tgcctgaccg
     2221 cagcttctga caaaatggtg actttcccga tcaatagcac taaagcggac ctgtttgagt
     2281 ctcagcaacc ggttagccag tgcagtgacg tggtgcccgt tacggtaatt cgcctgcagg
     2341 atattaacgg ttgtgtcgtt tccctgttgc tgccggaaaa agagactttt gagcgaactc
     2401 catgagaaaa agctcggatg aactatctga ttggcatcac cacagagtaa aaactgaccg
     2461 ggatgacgta gcgttttcag taccagttgc agttgaacag gcgtcatatc ctgaacttca
     2521 tcaacaaaaa tgacatcata acggggagtt gccaacgaaa gatactgatg actgagcaga
     2581 ttactgtcat actgatgcga ctgtttcagc catgcgatat aacgttcaaa caggacatag
     2641 accgtcgggc ggtcctccat gccatagagc gactgccgta tccccagtga cagatacgcc
     2701 tcccgggaca gcggcccatt cccggaggca accgcaccga tcacgcccct gaactcctca
     2761 tacagggtat gggcagcccc cagtgcggcg atagccctgt tgcggggcag ccagtcgctg
     2821 aacgcactga aagtcacctc cctgccttcc ggaatgcgca gagtttccag aaattccgtc
     2881 agcgaaagaa agtcaatatt ctgtacctca ctcccttccc ctgaagcgtc atagagagta
     2941 cgcgcttttt ccaccagaaa tgaggaaagg ctgagataca gtatatcacc agcagcctgc
     3001 ttcatttttt ccagtaccag cgccgtctta ccacttcccg ccgagccaac aatcaccagc
     3061 ggtagcgggt gttcgtacag tgcctgctga tcatcatcaa aggacagcat tttatccagg
     3121 cggtggaaac gcccatgaga cggattgata tacgtcagcg cttcagtcgc gatatcaaca
     3181 ggatccggca ctggttgttg ttgtaagcgc ccttcatcaa tcaccacgtt tctgcgaaga
     3241 aaacgtgaag tgttataggc atgattgcga atatattcca gaacaaggca gatagtttca
     3301 ccgtggtagc gatacagcga aaacagtaat cggtcccgga tactgagccg ggcgcggtaa
     3361 agattggtgt caattttacg aacatcagcc tgaacaaagt tatcggtggc aatagcctgt
     3421 gcaaattttt tataacccgg gatcgtgtca ggattcagag tatgataaag acagattttc
     3481 atggctgccg gtttccgtcg tgcaaaaaac atgctgattt tacgaaggaa tgccttactg
     3541 aacaaccttg cccggaatac cccccgaaat cagatggaac aatcagaaaa atgctctgca
     3601 gttatggaat aaagaaggtg gggtggtacg ccgggagggc gatccgctgg aaatggcgca
     3661 gtagctgcag gcctgtcatg atgccggaat agaagttcgt gtacaggtta atgaaagcgt
     3721 caccccgtaa ataaactctg cgctgaaatt cctttttttc attggagcac ctgtgttgtt
     3781 ctgaggtgaa catatcacct ctgttcaggt agccaaattc agtgtgccac ctcaaggttc
     3841 ttagtgagtc gtttttaaaa gatatgataa tatatcattg tttatgaatt gttgatgttt
     3901 aatttagtaa attatatgaa atatgccatg ggtttgctat gttattcatc cagtatctgg
     3961 aaataaaatg caccagcttt attgggtttt ccttttgtct ctgcttacta ggtgccagaa
     4021 actttaaatg ttaaacgaag ggggcatttt gttcaaagcc aatcatgttc cggtattaat
     4081 gtatcatcat gtcagtcatt gccccggact ggttacactt tctcccgtga cattccggaa
     4141 gcaaataaaa tggttggctg aaaacaactg gaaaacactg tcatcagatg aactggagtt
     4201 tttttatcgg ggcggaaaac tcccccggaa aagcgttatg ttgacatttg atgatggata
     4261 ccttgacaac tggtttcagg tatatccttt attaaaagag tttaatctca aagcacatat
     4321 ttttttaatt accggtttta ttgggaatgg tccggttcgt cactctcctg gaaaggaata
     4381 ttctcatcgc gattgcgaac atcaaatagc gacagggaat gctgataatg ttatgcttcg
     4441 ctggtctgaa gtaaacgaaa tgttgcaatc tggtctggtc gaatttcatg ttcatacgca
     4501 tactcataca cgctgggata aaaagttcag ttcacgagaa gaacagtgta agcatcttcg
     4561 gcaggacttg ttgagcggga gagaatgttt gaaagaaatg acaggaaaat gcagtaaaca
     4621 tttgtgttgg ccggaaggtt attataacaa ggattatatt caggtcgctg aagaacttgg
     4681 attctattat ttatatacaa cagaaagaag aatgaatgct cctgctaaag gaactacacg
     4741 gataggaaga ataagcacca aagaacggga aagttgcgca tggctgaaaa gacgactttt
     4801 ttattataca acaccgtttt tttcatcgtt gctggccttt cataaggggc caagattacc
     4861 ggatgattaa taatgaatat actatttacg gaatcatcac cgaatatagg tggtcaggaa
     4921 ttacaggctg ttgctcaaat gaaggccctg aagaaaatgg ggcattcagt tctgcttgtc
     4981 tgcagggaaa acagcaaaat tgcttttgaa gccagtaaat tgggaattga tatcacattc
     5041 gcgttatttc gaaacagtct tcacatccct actgcatgga gattactcgg aatagttcat
     5101 ggttttcagc ccaatgcaat cgtttgtcac agtgggcatg atagcaatat tgttggttta
     5161 gtacggttat tcactaggaa acatccattc agaattatca ggcaaaagac atatttgaca
     5221 cgaaaaacaa aagttttttc aataaatcat ttttgcgatg aggtgattgt tcccggaaca
     5281 agtatgaaga cacatctgga gcaggaagga tgtcgaaccc gggttactgt tgtgcctcca
     5341 ggctttgatt tccagaaatt atacgttgat tcccgaaaca gtttgcctac aaatgttctt
     5401 tcttggctgg cgtcccgaag gggatgcccc gttattgctc aggtaggaat gttgcgcccg
     5461 gaaaaagggc acgaatttat gttgaattta ctgttccatt taaaaatgaa tggacgacag
     5521 ttctgttggt tgattgtggg gtctggttcg cctgaactgc gggagcattt acagtatcag
     5581 attgacagta tgggcatgca tgatgatgtt tttattgctg acaatgtttt tcctgccgcc
     5641 cccgtatatc gggttgccag tctggtggtt ctgccttcag aaaacgaatc gtttggtatg
     5701 gtactggcag aagcatcggc attttctgtg cctgtactgg ccagtcagat tggtggaatc
     5761 cctgatgtta ttcagaacaa ccagaccggg acattgttac cagcaggtaa taagcacgca
     5821 tggatgtgcg ccctgaatga tttttttaat gaccctgggc gtttttatca gatggctcgc
     5881 caggcaaaac aggatataga agagcggttt gatattaata aaactgcgtt aaaaatactc
     5941 acattagcga agcacaagta acaatatgtt ttctgtaagt aacttatcat ttatcggttt
     6001 ccttaaaagg attgtttttt cctcagattc actcccgggg aagtgggaac acagaaaatt
     6061 ccggttcatg tacattttgc gatgtgctat aaatccggtt gccagtattc gatattatta
     6121 cgaactgcgt tccttgcagt gcattgagga tattctggca atacaaccca cgttgccagc
     6181 aagaattcat cgaccttatt tgcataaagg ggggcgagcc tggtcccggg ggcaatatat
     6241 tcttgagcat tatcgctttg tgcaaaattt gccagaaaaa tattctgaat ttttgtttcc
     6301 acaaaaatcg gtatcattag tccaattcat cgggaaagat ggagaggatt ttgatatcca
     6361 gtgttctccc agtggttttg accgggaagg agagttgatg ttgtcattgt ttttcaatca
     6421 aatagtgatt gcccggttaa cattttcagt cattctgact caaaatggac acaccgcttt
     6481 tattggcgga ttacagggag cccccaaaaa taccggacct gatgttattc ggtgcgcaac
     6541 ccgggcctgt tatgggcttt tcccaaaacg gattattttt gaagcatttt gtgcgttgat
     6601 gaaagcttgt aatgtctctg agtgtctggc agtcagtgag cacagtcatg ttttccggca
     6661 attgagatac tggtatcaga aacgcaaaac ctttgttgct gtctacagtg atttctggga
     6721 gtctgtagca gggaaaacct gtggtgactg gtacaaatta ccaacacagg tggttcgaaa
     6781 gccgctaagc aatatagcta gtaaaaaacg ctctgagtat cgaaaacgat acgctttgct
     6841 ggattatatc catgaaactg ctatccgctc tttggatgca tatcctgtaa actcagaaca
     6901 tcaggactta aattaaggtt tgatgtattc tctgaataaa atattaatga tgattttggt
     6961 aggggcattc gcactaaata atgaaaaaat acaaatccga gtttattcct gaatttaaga
     7021 aaaattatct ttcccctgtt tactggttta catggttcgt tttgggaatg attgcaggta
     7081 tttcaatgtt tcccccttca ttcagagatc ctgtcttggc caaaataggg cgttgggtgg
     7141 gcagattgag cagaaaagct cgtcgcaggg cgacgattaa tttatcgctt tgtttcccgg
     7201 aaaagagtga tacagaacgg gaaataattg tcgacaatat gtttgccaca gcattgcaat
     7261 ccatagtgat gatggcagaa ctggcaattc gtggtccgga aaagttccag aaacgagtgt
     7321 tctggaaagg cctcgaaatt cttgaggaaa tccggcacaa taacagaaat gtgatttttc
     7381 tggttcccca tggctggagc gtggatattc ctgcaatgtt gctggcagct cagggggaaa
     7441 aaatggccgc catgtttcat cagcaacgaa atccagtgat tgattatgtc tggaattctg
     7501 tacggcgtaa attcgggggg cgcttacatt cccgggagga tgggataaaa ccatttattc
     7561 agtcagtacg ccagggatac tgggggtatt accttccaga tcaggatcat ggtcctgaat
     7621 acagtgaatt tgctgatttt tttgcgacct ataaagcgac attaccaatt attggacgtc
     7681 tgatgaacat cagtcaggct atgattatac cgcttttccc ggtttatgat gaaaaaaaac
     7741 atttcctgac tattgaagtt cggccaccaa tggatgcatg cattgccagc gcggacaata
     7801 aaatgattgc ccgacaaatg aacaaaacag tggaaatttt ggtggggtca catccggaac
     7861 agtatatctg ggttttaaaa ttgttaaaaa cgcgcaaatc aaacgaagcg gacccgtacc
     7921 cttgaaatta taaagtacag gtatttccac tagttgtttc ttacaggtta ccaatcgaaa
     7981 cacatccccc ttccgtgtgt tgcccgtttg gcacggagga ctgtgtagat gcagttcgtt
     8041 aaatttaatt tatgcctaaa tagctgcgct taataccgct acacttttgc cagcccatgt
     8101 ttgcctccgg ggaatgggct gacggtttcc ataaaatggc gaactttttt caacagttgc
     8161 cacattgagc ggcactgatg attacgcgtt attgtgtcgt gaagtgcctg ccatagccgt
     8221 tcaacatgat tcacccatgg cgagtaaacc ggctgataaa tgcccctgaa cttcgggttc
     8281 tccttcagcc agctctgtgt ttcccggctt ttgtggataa tgtagttgtc cacgatcagc
     8341 gtgatggttt ttgcccgacg gtatgtcgct ttaagccgct tcggcaggct gat
//
LOCUS       DAETPZ010000136         8330 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.137, whole genome shotgun sequence.
ACCESSION   DAETPZ010000136 DAETPZ010000000
VERSION     DAETPZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.137"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..892)
                     /locus_tag="K0W59_003717"
     CDS             complement(<1..892)
                     /locus_tag="K0W59_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBI3024570.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPS
                     SVNVLSELPGRTVPYEAAEIRPQVGGIIIKRNFIEGDKVNQSDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGS"
     gene            952..1230
                     /locus_tag="K0W59_003718"
     CDS             952..1230
                     /locus_tag="K0W59_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024571.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1231..1758)
                     /gene="gadE"
                     /locus_tag="K0W59_003719"
     CDS             complement(1231..1758)
                     /gene="gadE"
                     /locus_tag="K0W59_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HBI3024572.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2557..3129)
                     /gene="hdeD"
                     /locus_tag="K0W59_003720"
     CDS             complement(2557..3129)
                     /gene="hdeD"
                     /locus_tag="K0W59_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HBI3024573.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3384..3716
                     /gene="hdeA"
                     /locus_tag="K0W59_003721"
     CDS             3384..3716
                     /gene="hdeA"
                     /locus_tag="K0W59_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HBI3024574.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3832..4158
                     /gene="hdeB"
                     /locus_tag="K0W59_003722"
     CDS             3832..4158
                     /gene="hdeB"
                     /locus_tag="K0W59_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HBI3024575.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4222..4868
                     /locus_tag="K0W59_003723"
                     /pseudo
     CDS             4222..4868
                     /locus_tag="K0W59_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4910..5440)
                     /locus_tag="K0W59_003724"
     CDS             complement(4910..5440)
                     /locus_tag="K0W59_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3024576.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5596..6162)
                     /gene="slp"
                     /locus_tag="K0W59_003725"
     CDS             complement(5596..6162)
                     /gene="slp"
                     /locus_tag="K0W59_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HBI3024577.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGLGAPYYTNAVSQVTPELVK"
     gene            complement(6409..7632)
                     /locus_tag="K0W59_003726"
     CDS             complement(6409..7632)
                     /locus_tag="K0W59_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024578.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8213..>8330)
                     /locus_tag="K0W59_003727"
     CDS             complement(8213..>8330)
                     /locus_tag="K0W59_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBI3024579.1"
                     /translation="LTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2426 a         1614 c         1734 g         2556 t
ORIGIN      
        1 ggctaccttc atccactaat gccgtgacat acatgccagg cagcaagtca ccatttgggt
       61 tggggaaaat cgcccgtaac gtcacggagc ccgtggtttc atcaaccgtc gggtcggaga
      121 atttcagcgt gccggtctgg ctgtaacgtt taccattttc cagattgagc tgtactggcg
      181 tactgccctg aacctgtttg atttgcccac tggcgacctc ttctttcatg cgtaagaaat
      241 cttgcaccga ctgcgtgaga tcgacataaa tcgggtccag acgttgtacg gtaaccagcg
      301 aatctgcctg attagcggta acgagtgcgc cgacggtcac cgacgatttc ccgctgacgc
      361 ccgtaatcgg cgaggtgaca ttcgcgtatt gcagattgat cgtcgcctgt tcaacagccg
      421 ctttggcgac ggtgacattg gcttctgctt cattcagctg ggtgcgcgcg gtgtcgtaat
      481 cctgacggct aacgtagttg gtcttcagca acgatgcctg gcggttaaag gtgatgcggg
      541 cattgctggc ggtagagagc gctttcgcca gcgagccttt ggcggagttt agctcggcct
      601 gtaaaggtgc aggatcaatc tgatacagcg aatcgctctg gttcacttta tcgccttcga
      661 taaagttgcg tttaatgata ataccgccca cctggggacg tatctcggca gcttcataag
      721 gaacggttct accgggcaat tcgctcaaca cattgaccga actaggggag agtgtgacga
      781 caccgacctc aggcgtcatg gcggcggcgt tttccgccga tttgtcatcg caggcggtga
      841 gcatcgcgcc gcagaataac aacggtatta acagctttct tcttctgttc attttagtcc
      901 ctgaaaattc ttgagaaata acggttcttt aaattgcgcg cgaggagaga tatgagggat
      961 ttacagacca gcggcatagt ggggttgtca gccagtaagg tgggatacag gcacagtgat
     1021 cgacatggtg aggtcaacga caaaaaaaat gttgccgttc tgccaacagt tcctgccagc
     1081 attcgggcaa cgaagggttc tactggtgga tacacatacc aggggaatca agtcgtttta
     1141 gcctctcctt tagtaatcac cgggggtaat gaaatcagca tctgcatccc tcgtcatgcc
     1201 agccatcaat ttcagttgct tatgtcctga ctaaaaataa gatgtgatac ccagggtgac
     1261 gatgtcgctc atacgtctta gtttcagctt gtacataatg ttgaatttct gaatctttaa
     1321 tgtttgttgc gaaatattga gcgtcgacgt gatattgctt tcatttttat ttttcagaat
     1381 cagttgcata attttgaact gattatgtga aaaacataat tgtgaattct tatggggcaa
     1441 gtgtttacca taagtaatct caagaagatt tttgatactt tctttgcggc taacaaaata
     1501 aataggcgct ttagctttta gtttactgat gtgataaggt gccaaaacaa taattctctc
     1561 ggcatctaat ttctccagaa atttaatcgc ttcttcatca aggatatgat tgtgataggt
     1621 atcaatgata accttgaagg gtttattgcc tacttttttg atctctgaca gggaattgat
     1681 ttttatcatt tcgtgattat ctttcaactg ccaaaagccc tgtaaaagaa aagaatcttt
     1741 cgtcatgaga aaaatcataa cttgctcctt agccgttatt gtttcgaaca tgtcatcctt
     1801 ttcattattt acatccttgt ccgaatcgtt gttcaatata gtaaacgcct ataagaactt
     1861 tgcccttcgt gaactaatac gtctattaac actaatcaat acttcagatt tatccatcac
     1921 gcactccata tattgatcct aagtaagatt cctggttgtt atcagcttgt aaatagtaaa
     1981 caaaaagtta acgtttgcgg attactgccc aagaataagt tatcaataac cagtttgtga
     2041 tctctgaaga atattactaa agttaaaatg taatccgatt taaatatcga gtctccttgt
     2101 ttcgacttaa gctggcaatt ggattgccag ctttctttta acgcattgaa attacctgag
     2161 taaataacgc atttatgtct tcaggttttt atatagcgtc tggagacacg gatatttatg
     2221 caatgtaatc aacgccagtt gcaaaaaaat gaacccggat tacttattta accgcccata
     2281 gcatcaacgc ttacggaact ctttgttcac tttcccacag cacttttaaa acattaaacc
     2341 tgactacgga aaatatcagc catgataatg tttgaaatgg ctaatttgcc atggagtgaa
     2401 aaaaattaga tgaaattcag taggttgaaa taatcactag caggtaatta tttcaatgat
     2461 agtgcgcaat tgatctacaa cactgcgtag cggagagagt attaatcgga tcatagtcac
     2521 atcaagtgac tatgatccgg gtgacaaccg gggtaattat tgctgcttaa cgaacaaact
     2581 ggcgaagctg aacaggctgg cggcgctaaa tatcagttca attcccacca gtgtggaaac
     2641 cagcgttaca gacaccatcg gcgttgcacc aaggaatatc caggcaatga cgatatccag
     2701 cacaccaata acgagctgta gccagctgcc tttcattgaa cgctgacgat accaactcat
     2761 caggcgaata acccctgcaa cacagaacaa accggcaata aatgccgcaa tggcaaaaat
     2821 gcccagctcc ggtgcacgga tgaagaaata gccgatcaat aaataggcga ctgcgacgag
     2881 gaaaccggat aataccggcc agaaattatg actgcggttg ctgaataacc cgacaataag
     2941 cgcaataccc gagcagatta ataatgcacc cactactgtg cttaaaatat cgccagagac
     3001 gaacgggaaa ctgatacaca gcaacccgac gataaacagc agcacggcaa taaactggat
     3061 tgctctgcga tgctttttca gcatctccag atcaaacttc aaaattgttg ccttatctat
     3121 atataacata gaaccaccct ataaaattaa gaagaaaatc ccctgctatc aatctatgcc
     3181 aaaaacgcgt ctaagaatgc agtcgattta ataaaaattt cctaattgca gtatctgatg
     3241 catctgtaac tcattgtatt gaaataaaaa tatctgattt tgatattttc catcaacatg
     3301 acatatacag aaaaccaggt tataacctca gtgtcgaaat tgattcgtga cggctctttc
     3361 actttatagt tgaggatatt acgatgaaaa aagtattagg cgttattctt ggtggtctgc
     3421 ttcttctgcc agttgtgagc aatgcagcgg atgcgcaaaa agcagctgat aacaaaaaac
     3481 cggtcaactc atggacctgt gaagatttcc tggctgtgga cgaatccttc cagccaactg
     3541 cagttggttt tgctgaagcg ctgaacaaca aagataaacc agaagatgcg gttttagatg
     3601 ttcagggtat tgcaaccgta accccagcta tcgttcaggc ttgtactcag gataaacaag
     3661 ccaactttaa agataaagtt aaaggcgaat gggacaaaat taagaaagat atgtaattcc
     3721 gggaatgcgt tacatcgtac ttccttgcat attgaacagg ccggaatatc ttctttaaaa
     3781 gcagctattc ctcctgttca tatataatct ctatattgaa tgggttacaa aatgaatatt
     3841 tcatctctcc gtaaagcgtt tatttttatg ggcgctgtag cggctttgtc actggtgaac
     3901 gcacaatctg cgctggcagc caatgaatcc gctaaagata tgacctgcca ggaatttatt
     3961 gatctgaatc caaaagcaat gaccccggtt gcatggtgga tgctgcatga agaaacagta
     4021 tataaaggtg gcgataccgt tactttaaat gaaaccgatc tcactcaaat tcctaaagtg
     4081 atcgaatact gtaagaaaaa cccgcagaaa aatttgtata ccttcaaaaa tcaagcatct
     4141 aatgacttgc cgaattaatg aggtacaagt aaaaaggagt agcaagttga gccatcttgc
     4201 tgctcctttt tgcattttta tatgacagca gaatttatta tacgtcttat actcgcggca
     4261 attgcctgtg gcgctattgg catggaaagg caaatgcgcg gcaaaggagc agggttacgc
     4321 acacatgtat taattggcat gggaagcgcc ctgtttatga ttgtttcgaa atatggtttt
     4381 gctgacgtgc tgtctttaga tcacgtcgga ctcgacccca gccgtattgc tgctcaggtg
     4441 gtgacgggcg tcgggtttat cggtgcaggt aacattctcg ttcgtaacca aaatattgta
     4501 ggtctgacga cggcagcgga tatctgggtg accgccgcat aggtatggtt attggcagcg
     4561 gtatgtacga actgggtatt tatggttcag tgatgacctt gttggtgctg gaagtcttcc
     4621 atcaattaac cttccgcctg atgaacaaaa attaccattt acagctgaca ttagtgaacg
     4681 gtaatacggt atcaatgctc gactggttta agcagcaaaa aataaaaact gatttagttt
     4741 cattgcagga aaatgaagat catgaagtgg tcgccattga tatccagctt catgccacaa
     4801 cgttggtaga agacctgttg cgattattaa aaggtatggc aggggtgaaa ggcgtttcaa
     4861 ttagctaatc tgtgtggcag cgtagccaga ctcaccgtaa gcctgaaatt cacaccagat
     4921 aatcaatatg ctgatggcgt accagctcat tgatccgctt aagattgagg atcatcatga
     4981 tattgtgttt atggcagtag aacgttttta gcgatatttt cagtttctcc agaatatctt
     5041 cagttgtcat ttgattcgca atgcagctca gaacctgatg ttgcttgcgc gaaagtgaca
     5101 cattcgcttc aggcacagag cagtaactgc cattgatgag tgcgacaaaa tcaatgatag
     5161 tgatagtccg cggaacaaag gtcaccggta ctttccccag acagcgttta atattaaagg
     5221 gggagaaaac aatgacatgg acaggtttta atcgttcaat ttgagtgaga aaattagaat
     5281 gaaatacatt attcattaat gtatcaataa tgacgaaggc attctgatgc aacattacgt
     5341 cgatctcttc ttcgttctgt atatgaacga cattaccttt actcagaatg tttttcatcg
     5401 cggtgaagaa catcgtatcc ctggtaatta taagaaacat aaccgactcc tggtgcggac
     5461 attaacttta taatcagttt gccaaaggta attaacagga cagggcttat aataataata
     5521 gttagcttac tgtatattaa ttttatgtaa tacatgatgc agatgctgag aaagcatctg
     5581 catctttcgg tggtgttatt tgaccagctc aggtgttacc tgactcaccg cattggtgta
     5641 gtaaggcgca cccaagcccg gttccggcca gaatgcacca tagccgtaat cccacaggtt
     5701 ataggtggta ttaaccactt ctctcaaatg ccacacctgg atgccctgca tattcacttc
     5761 caggaagtta tacgggactt tattgataaa gcctggttgt tcaccctgaa tggtaccgag
     5821 gatggtaaca aagtgattac gatagttcac tggatcaagg aagccgcttt gtctggcgag
     5881 cagtcggccc tgatagttgg cttcaatatc aggcttcgca tagctatcca acggtaatac
     5941 agcgatttct aacaacgtat ccgttttgcc attgataacg ttgataacct tcccaccaaa
     6001 gcgcgcttgt tgaccaacat ataaccccgg ctggttatga acagcaacaa aacttttttg
     6061 aatatcaggt tggttattgc ctttgatatt ttgcggaatt gaactacatg cggcaagcaa
     6121 aaatgaaagg ctgaggatga gtgcaccttt tgtcatgttc atgttactat ccttatcaac
     6181 aaatattaaa accataatag atatttcttt atagttttca tctgattctg aggtgatatg
     6241 cctgatagta acgtaaacct gtctgatgtt tatatccctt ctgtttagtt caattccgtt
     6301 attttggggc gtggtatgag tatccatcgc ccaataatat aaccctttgt tttaaaatta
     6361 cataaaatct atttttattc aataattata aagtgctaat aatcaatatt atggtattat
     6421 attctgataa tgatttgata agcgaattct tttgccagat ggtatttcta tttccacccc
     6481 ataatttata tctttttcat taggcagatc tctctttaaa taaaaggggt atgaaatctt
     6541 ttcgggttga agtgtctcgt aatgaaatgc tatgaattca tttttagaca ttgttgccca
     6601 tttttgaatt ttattgtata gagagggtgt tgtaaactct tgaagttgtt tataacatat
     6661 ttttttgaac tcttgcttat attttaatat gtcatcaaat gaaagattca ctacctttgc
     6721 ctctaagaaa tcaattattc tcgaagcaat ttccgctttc gcgcgtaaag agttgtcatt
     6781 aatagttaac aatgtagaaa acaagccatt gatttgtttg tcaagatgtt gttcatattt
     6841 atgtagtttg gggatgggat tttccttgtg tgaaaaaata gtgttaaata aatttgcgtc
     6901 agtgaaattt atattaagta gatcttcaaa ataaaatttt gataaggtta ggactagctt
     6961 ggacatatct tgccctgtca tgttgaaatc aataaagtcg catccagcaa gggcttccat
     7021 cctattttca tttttactgt gctcaatata cagcttcgcc atttttaagt acaagctttg
     7081 aatgtctctt atatcggttg gtaagtcagc tcgttgccca gcaaaatttt tattatcgga
     7141 agaaacggtt tggacactga atctttcggg tattccttct tctacgagaa ttttagttat
     7201 gattggattg ttgggttctg gattaacgac aacaatgatt tgacatacag aatccttatc
     7261 cttttcgtat ctaatgttat gaatctgatt aagtattgat tgtgtctgtt gcgtaagtgg
     7321 tgtcgaactt atcgtttcaa tgttattaat aagacttaca agttgaacca caacttttga
     7381 gtagtctgca tttgtatatg tggtgttcgt gtggcatgca ttaatttgtt ggagttgttg
     7441 ttgcgctaaa tcaaattgtt cagggttgta tccttctcca gagaaggtgg aattaatggg
     7501 ttgttgttct tcccaattat cacaaggcca cttcggtgtg tctggcgttt taacattatc
     7561 ggtatttgtt gtgcagttat aaatatcgcc gtgatatgta tttattatac ctggggtaaa
     7621 gtcaattgac ataacattcc ttattcatca tctgttttat gctaatgttt ttaaattgga
     7681 taattagaac aggaatggat tttcttctac acacaataaa tataatgcgt tatatcttcc
     7741 tgatataatt acctttaaat tccctttcat ttccacccgg taatatttgt gtataaaaaa
     7801 agggcagtga tagtatcgta aaggaaaacg ttaacaacta tcaaaaagcg ctcattttaa
     7861 aaagttatta tgttctccga tagtgtgtga agtaatattt ttgtgatgtt ttagatgtgt
     7921 aataatgaac caggttaaag tgtatttaaa aatgtgttgt attagcgact gaaatctgcc
     7981 ttttaaaaag gaggagggta ctgagacaaa gagtcattgc ttctccgata aaatgtcaga
     8041 gaagcatgat tacgtggtta ttgtctgttg atgacttgtt gaccgagccg tcatcattct
     8101 caatgcatta gttaaatcat ttgtgatttt taaggattat cctgtctatg attttctact
     8161 tagaaggata tggcactaac tatgcatctt atagtttact gctggcatct atttaaaata
     8221 gcaattgagt caaatagccc atcaagatag ccatacctaa tataaccgtc aggaatgcag
     8281 ctatcatcgg cattctgaac attgatttaa gtaatatcac ttccgtcagg
//
LOCUS       DAETPZ010000137         8320 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.138, whole genome shotgun sequence.
ACCESSION   DAETPZ010000137 DAETPZ010000000
VERSION     DAETPZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.138"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            334..3183
                     /locus_tag="K0W59_003728"
     CDS             334..3183
                     /locus_tag="K0W59_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBI3024580.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYATSRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRMGDLRNQ
                     SKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLYIDSTHA
                     SPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNCVNANGT
                     ANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLNHYESLL
                     GRASMILGYDITAGNSQLNVYVKTGAIREFSGDIEYLLNDSREKYSFKGNGWNNGVGV
                     SAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(3283..5202)
                     /gene="dhaR"
                     /locus_tag="K0W59_003729"
     CDS             complement(3283..5202)
                     /gene="dhaR"
                     /locus_tag="K0W59_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HBI3024581.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATMTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            5430..6500
                     /gene="dhaK"
                     /locus_tag="K0W59_003730"
     CDS             5430..6500
                     /gene="dhaK"
                     /locus_tag="K0W59_003730"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBI3024582.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGR"
     gene            6511..7143
                     /gene="dhaL"
                     /locus_tag="K0W59_003731"
     CDS             6511..7143
                     /gene="dhaL"
                     /locus_tag="K0W59_003731"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HBI3024583.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            7154..>8320
                     /gene="dhaM"
                     /locus_tag="K0W59_003732"
     CDS             7154..>8320
                     /gene="dhaM"
                     /locus_tag="K0W59_003732"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HBI3024584.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEE"
BASE COUNT         2174 a         2069 c         2075 g         2002 t
ORIGIN      
        1 cccataacta attaggatta ttacaaaaaa tcttcaccaa attttttaac cgctgtttgt
       61 tgtaatattt ttgccatatt catagtaacg aattggaata agtaacgaca acttagggaa
      121 taactcgata taaactcacc tttgtaaggg gggtgttaaa tatatattaa ttcctgtgtt
      181 cctgattttg cccggtaata gagaattatt ctcagagaag ctcatcatga ttgcaccata
      241 cagatatcat gatgataaga ggatattatt cattgactta aacaaaatga caaaatccat
      301 agacccatag cctgggtact atataggagt ggtatgggca tcaaacaaca caatgggaat
      361 accaaagccg atcgtctcgc tgaattaaaa atccgttcgc cctcaattca actgataaaa
      421 tttggcgcta ttggtttgaa tgcaattatc ttttcccccc tgctgatagc tgctgataca
      481 ggaagtcaat atggcaccaa tattactatt aatgatggtg acagaattac aggagatacc
      541 gccgatccat caggaaacct ctatggtgta atgaccccag caggaaacac gcctggcaat
      601 atcaacctgg gtaatgatgt caccgtcaat gtcaacgacg cctctggata tgcaaaagga
      661 atcattattc agggcaaaaa cagctccctg acagctaacc gactcacagt agatgttgtt
      721 ggtcaaacct ctgccatcgg cattaactta attggtgact atacccatgc tgacttaggc
      781 acaggcagca ccattaagag taacgatgac ggcatcatta ttgggcatag ctcaacacta
      841 acagccactc aattcaccat tgaaaactcg aacggtatag gcctaaccat caatgactat
      901 ggcaccagtg tcgatcttgg aagcggaagt aaaatcaaga ccgatggaag tacaggtgtt
      961 tatatcggtg gtctcaacgg caataacgcc aatggtgctg cgcgttttac ggcgacagac
     1021 ctgacaatcg atgttcaggg ctacagcgcc atggggataa acgtacagaa aaactctgtt
     1081 gtcgatctcg gaacaaacag taccattaaa accaatggcg ataatgctca cggcctctgg
     1141 agctttggcc aggttagcgc gaatgcactc actgttgatg taactggagc cgcggccaat
     1201 ggcgtcgaag ttcgtggtgg tacaaccact atcggtgcag atagccatat ttcttccgcg
     1261 cagggcggtg gcctcgtcac cagtggttca gacgcgacaa tcaatttttc tggcacggca
     1321 gcgcaacgaa acagcatctt ttccggcggt tcttatggtg cctcggccca gacggcaacg
     1381 gctgttatca acatgcaaaa taccgatatt acggttgatc gtaatggcag tctggcgctg
     1441 ggtttgtggg cgctcagcgg cggtagaata accggagaca gtttggctat caccggcgcg
     1501 gcaggagcca gagggattta tgccatgacc aacagccaga tcgacctcac gagcgatctg
     1561 gtcattgata tgagtacacc cgaccagatg gccatcgcaa cgcaacatga cgatggttat
     1621 gccaccagcc gcatcaacgc ctcgggtcgt atgcttatca acggtagcgt tctttccaaa
     1681 ggtgggctaa tcaatctgga tatgcaccct gggtcggttt ggacaggttc ctccctcagc
     1741 gataatgtca atggcgggaa actggacgtt gcaatgaata acagcgtctg gaacgtaaca
     1801 agtaattcta atctcgacac gctggcgctg agccattcaa ctgtcgattt tgccagccac
     1861 gggtcaactg ccggcacatt taccacatta aacgtagaga acctgagcgg taacagtacc
     1921 tttattatgc gtgctgatgt tgttggcgag ggtaatggcg ttaataatag aggggattta
     1981 ttgaatatca gcgggagtag tgctggtaat cacgtattgg ctatccgcaa ccagggcagc
     2041 gaggccacaa cgggaaatga agttctgaca gtggtaaaaa ccactgacgg cgcggcctcg
     2101 ttcagcgcgt cttctcaggt tgagttgggg ggatatctgt acgatgtgcg taaaaatggc
     2161 actaactggg agctttacgc ttccgggaca gttccggaac cgactcctaa tcctgaaccc
     2221 acaccagctc ccgctcagcc tcccatagtc aaccccgatc ctacgcctaa acccaccacg
     2281 accgcagatg ctggcggcaa ttatctcaat gtcggttact tattgaacta tgttgaaaac
     2341 cgtacgctga tgcaacggat gggtgacttg cgaaatcaga gtaaagacgg taatatctgg
     2401 ttgcgcagtt atgggggaag cctggactcc tttgccagtg gcaaactgag cggctttgac
     2461 atgggttaca gcggtatcca gtttggtggg gataaacgtc tctctgatgt aatgccgttg
     2521 tatgtcggtc tgtatattga ctcaacacat gcatcgccgg actatagcgg aggcgacggt
     2581 accgcacgtt cagactacat gggaatgtac gccagttaca tggcacaaaa cggtttttac
     2641 agcgatctcg ttataaaagc atcgcgccag aaaaatagtt tccacgtact ggacagtcag
     2701 aacaactgcg ttaacgccaa cggcactgcg aatggaatga gcatctccct ggaagccggg
     2761 cagaggttca acctgtcccc tactggttat gggttctata tagagccgca aacccagctt
     2821 acatacagcc accagaatga gatggctatg aaggcgagta atggcctcaa tatacatctg
     2881 aatcactacg aatcgctgct ggggcgtgcc agcatgatac tggggtatga catcaccgca
     2941 ggcaacagcc agctgaatgt ctatgtgaag actggcgcta tccgcgagtt ttcaggggat
     3001 atcgaatatc tgttgaacga ctcccgggag aagtacagtt tcaaaggtaa tggctggaat
     3061 aacggcgtgg gagtcagtgc acagtataac aaacagcaca cattctatct cgaagcggat
     3121 tacacgcagg gtaacctctt tgatcagaag caagtcaacg gaggatatcg cttcagcttt
     3181 taagtgcggg atagacaagc ccttaaacga tgctgaaaat tgttagcgtg cgccggaatg
     3241 aaatacagat cactctgatg tatcagaaat cgtttctgtc tttcataccc ggcgcttaaa
     3301 ctgccctgca tcaatgccat gttgcttcat tttccgccac agcgtagtgc ggccgatccc
     3361 aagtaaagcc gacatttcct gaatgcgacc gcctgtgacc tgggctgcgt taataattgc
     3421 ctctttttca acttccgcaa atgacagact ggtggaaagg cgggtggcgc tgacatcatc
     3481 tgtcgcctgc tcggtaaaca gatgttcagg taaatcactg acgcgaatgc gcccgttatc
     3541 actactcaga gcaagattct cgatgacgct gtaaagttca aaatcgttgc ctggccatgc
     3601 acaagaaact aggcgagcga gggcatcgtc atcaattttc agccgcgtag agaagcgttt
     3661 ttcaagactg cgtaatttgt tattcaccag cgccggaatg ctgccacgcc gcatacgcag
     3721 aggcgggatg gtaatttcaa atgcatgcag cgcgtaatac agctggcgac taaaacgatt
     3781 ttgttccacc agcattgcga ggtccgcggt cattgttgca atcactttga catcaattgg
     3841 tattaaacgc cgcgcatcca gtcgcgtgat aaccccctgc ttgataacct gcagcaaagc
     3901 agactgtaac tccaccgcca gatattcaat cttttcaaga aacagcgtgc cgccgtgtgc
     3961 cagttccagc cgactcagac ggccattttc attgtccgtg cgatcgccac caataaattc
     4021 ttccgccagc gcagcatcac catataactc acaattgacg gcgatataag gacctgcagc
     4081 acgctcgctt tcattatgaa ttgcctgact tagcagtgcc ttgcccacgc cctcttctcc
     4141 acaaagcagg acaggaaagc tactgcgcgc cgcctggcga ccaaaatgaa tcaagcggcg
     4201 ggtttgcgga tcgtcctgtg gcatatgagc gaaggtatgg ctgacttttc ctaattgact
     4261 ggtcattaac tgccgcatct gttccacagg atggagcaac aaaataaagc tggttccctg
     4321 cgtttctatt atcggtttaa gggttatcac cgcatcaata aactggtgct ggctttcaaa
     4381 ggttgcttct acgtgtttga gcggatgtgc ctgttttatt gcttgttgca atacggcggg
     4441 taacgttaag agttcagtga ttgcccgtcc ctgacttgcc gtcgcgtcaa ggcgcaagac
     4501 ccgcgccgcc tgggcattaa taaattgcaa attaccctgc tcgtcccagc taatcacgcc
     4561 atcatccata ctttctaaca gggcattaag ttgatttaaa tgacggttag tttcagcgag
     4621 caaactgtcc gtcagcagta aattaccgac ctcgcgggcg attgccaacg tcaacggcaa
     4681 atcagctgcg gtagtttgct caaccggaca cgccagcgct attgttcccg tcaatcggcc
     4741 cttgctgtca aacaacggcg ttgcacaaaa ggcccagttc cagagtgcct gtttgaaatg
     4801 ttgatcggcc atcgttttca cggcctgacc agagatagcc gctaacgata gcgcacaagt
     4861 accaataatt ccctcggcgc aatacgtgcc gtcattgaat cccagtgcac ttagctgctg
     4921 caaggtttgc ggatcgccat tacggctgag aatgcaggcg gtttcatcga ggataaacag
     4981 cgcacactct cgcggtgcca tatattccca ggcatcttcc agcgcagcct gcccgagcgt
     5041 cagcatcgct ttcttacgcc gataaataga agcaaatgtc acgccctggg cctgatgtgg
     5101 tacgttccat gtctcccgtt tcatcagctt attgcatcgc tcccaggagg ttgcaattaa
     5161 gggagatatg ccccgaccat cgttgttaaa agcgccactc atatcccgca tcccttatga
     5221 caaaaggtgt tccggctcgc aattttaagt attaatacac gtaaaagttt caatatggaa
     5281 cattcattaa agcatatgtt tcactatgaa acataatttg ggctaatttt cttttccctt
     5341 gcctgatgca caatggattc atcggcagtc agtggtcgcc gtgtcgttga acatcatcca
     5401 tgccctaccg taattgctgg cgcaaaataa tgaaaaaatt gatcaatgat gtgcaagacg
     5461 tactggacga acaactggca ggactggcga aagcgcatcc atcgctgaca ctgcatcagg
     5521 atccggtgta tgtcacccga gctgatgccc ctgttgcagg aaaagtcgcc ctgctgtcgg
     5581 gtggcggcag cggacacgag ccgatgcact gtggttatat cggtcagggg atgctttcgg
     5641 gggcctgtcc gggcgaaatt ttcacctcac cgacgcccga taaaatcttt gaatgctcca
     5701 tgcaaattga tggcggcgaa ggtgtactgt tgattatcaa aaattacacc ggcgatattc
     5761 ttaactttga aacagcgacc gagttactgc acgatagcgg cgtaaaagtg accactgtgg
     5821 tcattgatga cgacgttgca gtaaaagaca gtctttatac cgccgggcga cgcggcgttg
     5881 ccaacaccgt attaattgaa aaactcgtag gcgcagcggc ggagcgtggc gactcactgg
     5941 acgcctgtgc ggaactgggg cgtaagttga ataatcaagg ccactcaata ggtatcgctc
     6001 tcggtgcctg taccgttcct gccgcgggca aaccttcttt tacccttgcg gataatgaga
     6061 tggagtttgg cgtcggcatt catggtgagc caggtattga ccgccgccct ttctcttccc
     6121 ttgatcaaac cgtcgatgaa atgttcgaca ccctgctgga acatggctca taccatcgca
     6181 ctttgcgttt ctgggattat caacaaggca gctggcagga agaaccacaa accaaacaac
     6241 cgctccagtc tggcgatcgg gtgattgcac tggttaacaa tcttggcgca actccgcttt
     6301 ctgagctgta cggcgtctat aaccgcctga ccacacgttg ccagcaagcg ggattgacta
     6361 tcgaacgtaa tttaattggc gcgtactgca cctcactgga tatgaccggt ttctcaatca
     6421 ccttactgaa agttgatgac gaaacgctgg ctctctggga cgccccggtc cacaccccgg
     6481 cccttaactg gggtagataa ggagaaagca atgtcactga gcagaactca aattgttaac
     6541 tggctcactc gttgtggcga tattttcaac accgagagcg agtatcttac cgggctggat
     6601 cgcgagattg gcgacgctga ccacgggcta aatatgaacc gaggctttag caaagtggtg
     6661 gaaaaactcc ctgctatcgc agataaagat atcggtttca ttctcaaaaa taccggtatg
     6721 acgctgcttt ccagcgtcgg tggtgccagt ggtccgctgt tcggtacctt ctttatccgc
     6781 gccgcacagg cgacccaggc acggcaaagc ctgacactgg aagagcttta tcagatgttc
     6841 cgcgatggcg cggacggcgt aatcagtcgc gggaaagccg aacctggcga taaaaccatg
     6901 tgtgatgtgt gggtgccggt ggtggaatcg ttacgtcagt ccagcgagca aaatctctct
     6961 gttccggtgg cgctcgaagc tgccagtagc atcgccgaat ccgcggcaca aagtacgatt
     7021 acgatgcaag cccgcaaagg ccgcgccagt tatctcggtg aacgcagtat tggtcaccag
     7081 gatcccggcg cgacctcggt gatgtttatg atgcaaatgt tggcgttagc cgcaaaagag
     7141 taaggaattg gtgatggtaa acctggtcat agtttcacat agcagccgac tgggagaagg
     7201 tgtcggtgaa ttagcccgtc agatgttaat gagtgatagt tgtaaaatcg ccattgccgc
     7261 gggaattgac gatccacaaa atcccattgg taccgatgcc gtcaaagtga tggaggccat
     7321 cgaatctgtt gctgatgccg accatgtgct ggtcatgatg gatatgggta gcgcattatt
     7381 gagtgctgaa actgcgctgg aattgctggc tcccgagatc gccgcaaaag tacgtttgtg
     7441 tgctgcgccg ttggtcgaag gtacactggc agcaacggtc agcgcggcct cgggggcgga
     7501 tatcgacaaa gttatctttg acgctatgca tgcgctggaa gccaaacgtg aacaactggg
     7561 tttaccgtcc tccgacactg aaatctctga cacatgtcct ccgtacgatg aagaagcccg
     7621 ttctctggcg gtggtcataa aaaaccgtaa cggcctgcat gtacgtccgg cctcccggct
     7681 ggtttatacc ttatcgacat ttaatgccga tatgttgctg gaaaaaaacg gcaaatgcgt
     7741 cacaccagag agtattaacc agattgcgtt actacaagtt cgctataacg atacgctgcg
     7801 cctgattgcg aaagggccag aagctgaaga ggcactgatc gctttccgtc agctggctga
     7861 agataacttt ggtgaaacgg aggaagtcgc tccacctact ctgcgtcccg ttctgcctgt
     7921 ttcgggtaaa gccttttatt atcaaccagt tttatgtacg gtacaggcaa aatcaaccct
     7981 gaccgcggaa gaagaacaag atcgattacg ccaggctatt gacttcacgt tattagatct
     8041 gatgacgtta acagcgaaag cagaagccag cgggcttgac gatattgccg caatcttttc
     8101 tggtcaccat acactgttag gtgatccgga actgctggcg gcggcaagcg aactccttca
     8161 gcatgaacat tgcacggcag aatatgcctg gcagcaagtt cttaaagaac ttagccagca
     8221 ataccagcaa ctggatgatg aatatctaca agctcgctat attgatgtgg acgatcttct
     8281 gcatcgcacc ctggtccacc tgacccaaac gaaagaagaa
//
LOCUS       DAETPZ010000138         8204 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.139, whole genome shotgun sequence.
ACCESSION   DAETPZ010000138 DAETPZ010000000
VERSION     DAETPZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.139"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            59..460
                     /locus_tag="K0W59_003733"
     CDS             59..460
                     /locus_tag="K0W59_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBI3024585.1"
                     /translation="MSSNFCERLEQWGNKSVNNGEERAIAVERIKEAYNSNMASLDLS
                     YLDLSELPPIPSTVNTLNLENNCLTCLDLADIASLVNINLSVNKINTITFPKEPKLEN
                     IYIDHNNLESLDLKNQHSLVNLEAQNNSLKK"
     gene            570..1901
                     /locus_tag="K0W59_003734"
     CDS             570..1901
                     /locus_tag="K0W59_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBI3024586.1"
                     /translation="MITDLILHNHPKMKTITLNDNHIAHLNAKNTTELEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYTNL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQ
                     LMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYTIKKAYTPYNIHTYKTE
                     EVVNQRNIKVKNMTLDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            2225..3613
                     /gene="narU"
                     /locus_tag="K0W59_003735"
                     /pseudo
     CDS             2225..3613
                     /gene="narU"
                     /locus_tag="K0W59_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
     gene            3695..7328
                     /gene="narZ"
                     /locus_tag="K0W59_003736"
                     /pseudo
     CDS             3695..7328
                     /gene="narZ"
                     /locus_tag="K0W59_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            7325..>8204
                     /gene="narH"
                     /locus_tag="K0W59_003737"
     CDS             7325..>8204
                     /gene="narH"
                     /locus_tag="K0W59_003737"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBI3024587.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKYIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEAASTEREVDLY"
BASE COUNT         2191 a         2032 c         1912 g         2069 t
ORIGIN      
        1 ttgtgtaaaa aataatgcca gttatcaact taataacgca ttacctaaca aagaaaccat
       61 ctccagcaat ttttgtgaac gactagaaca atggggtaat aaatcggtta ataatggtga
      121 agaaagagca attgccgtgg agcgaattaa agaagcttac aattcgaaca tggcatctct
      181 tgatttgtca tatcttgatt tgagtgaatt accgcctatc ccctcgacag ttaatacgtt
      241 aaatctggaa aacaattgtc taacttgtct tgacttagct gacattgcca gtctcgttaa
      301 tatcaacctc agcgttaata aaattaacac gataaccttt ccaaaagaac caaaactgga
      361 aaacatttat attgatcata acaatctgga aagtttagat ttaaaaaatc agcattcatt
      421 ggttaatctg gaagcgcaaa ataacagcct aaaaaaatga tatttctgat agttataaac
      481 tgaaatttct taatcttgat tataacaagc tagcatcgct ggatgtctcc cggcaagaat
      541 ctctgattga gttaagtgcc catcacaata tgatcactga ccttatatta cacaatcatc
      601 ccaagatgaa aacaatcact ttaaacgaca accatattgc acatttaaac gccaaaaaca
      661 ctacagaact ggaatattta aacttaagca ataacaattt actgccaacc aatgacattg
      721 atcaactaat atcatcaaaa catctttggc atgtattagt taacggcatc aacaatgatc
      781 cacttgcaca aatgcagtac tggactgcag taagaaatat aattgatgac actaatgaag
      841 tgaccattga tttatcagga cttaatttaa ccactcaacc accagggctg caaaacttca
      901 cctctatcaa tcttgataat aaccaactca cacattttga tgcaaccaac tacgatagac
      961 tcgtaaagct aagtctgaat agtaatgctc ttgagtcaat aaattttcct caaggcagaa
     1021 atgtaagtat tacacatata tctatgaata ataatgctct cagaaatatt gatatagata
     1081 ggctttcatc agttacttat tttagtgcgg cacataatca actagagttt gtgcaattag
     1141 aatcttgcga atggctgcaa tacctgaatc tcagccataa tcaattaact gatattgttg
     1201 caggtaataa aaatgaactc ttactgctgg atctatccca taataaacta acaagtttac
     1261 acaatgtctt atttcccaac ttgaatacgt tacttattaa caacaatttg ctttctgaaa
     1321 ttaaaatatt ctatagcaac ttccgcaatg ttcagacatt aaacgctgct aacaaccagt
     1381 tgaaatatac aaatcttgat ttcctgactt atcttccatc tatcaaaagt ttaagactgg
     1441 acaataataa aataacccac attgatacta ataatacatc cgatattgga actttattcc
     1501 ccataataaa acagagcaaa aacttaaatt ttttaaatgt ttctgggaag aacaattgcc
     1561 ctactatgca gctcatgtta tttaatttgt tttccccagc acttaagctt aatactggcc
     1621 tggcaattct ttcgcctggt gcatttgaag ttcactctga cggaatagat gtggataacg
     1681 aattgtttca ctatactatt aaaaaagcat ataccccata taatatacac acttacaaga
     1741 cagaggaagt tgtaaaccag aggaatataa aagttaaaaa catgacctta gatgaaataa
     1801 acaatactta ctataataac gattattaca atcaggcaat aagagaggaa ccgatagact
     1861 ttctggacag atcgttttcc tccaattcat ggccttttta gtgaataaat catcactaat
     1921 tctataagtg tctggtcttg ttgagaatat agttaatatg taacccttta gattcacgat
     1981 ctgaagggtt gaaacatgac atacgttcaa agattaaccc ccgtttgaag gttagcttgt
     2041 tagttacatt tagtaacaca tatctggcta cttaatcccc attctgcccc cgcggttacg
     2101 ctttcttatc tattctttca atcagcactt cacccaattc atccttaatt ctaataactg
     2161 gctattgcgc cttacctcct gttttgtctt ttttcattac caatgtgtgc atgtgaggaa
     2221 caatatggca ctgcaaaatg agaaaaatag tcgttatctt ttacgcgact ggaaaccaga
     2281 aaatccggcc ttctgggaaa ataaaggaaa acatattgct cgaagaaacc tctggatatc
     2341 agtcagttgt ctacttcttg ccttctgtgt ctagatgcta tttagcgcag ttaccgttaa
     2401 tctcaataaa atcggtttta attttactac cgatcaactc tttttattaa ccgcattacc
     2461 ctccgtttct ggcgcattat tgcgtgttcc ctactccttt atggtgccta tattcggtgg
     2521 acgccaatgg ccggttttta gtactgcaat cctgattatt ccttgcgtct ggctcggaat
     2581 tgccgtgcaa aatccgaata ctccttttgg gatatttatc gttatcgctt tgctatgcgg
     2641 ttttgcaggt gcaaactttg cttcgagcat gggcaatatc agtttcttct ttccaaaagc
     2701 caaacaaggg agcgctcttg ggattaatgg cggattagga aacttaggtg taagtgtaat
     2761 gcagctggtt gcaccgctgg tcatttttgt acctgtattt gcctttctcg gcgtcaatgg
     2821 cgtaccgcag gccgacggtt cggtgatgtc gctggcgaat gccgcatgga tttgggtacc
     2881 gctactggcg attgccacga tcgccgcctg gtcagggatg aatgatatcg ccagttcacg
     2941 cgcctcaatt gccgaccagc tccctgtctt acaacgcctg catctctggc tgctgagcct
     3001 gctttacctt gccaccttcg gttcgtttat cggtttttct gcgggttttg ccatgctggc
     3061 aaaaacccag ttcccggatg tgaatattct gcgcctggcg ttctttggcc catttattgg
     3121 tgccatcgcg cggtcggttg gtggtgctat ttccgataaa ttcggcggcg tgcgggtgac
     3181 gttgatcaac tttattttta tggcgatttt cagtgccctg ctgttcctta ccttaccggg
     3241 cacaggctcc ggtaatttca tcgcctttta cgccgtattt atggggctgt ttctgaccgc
     3301 gggtctggga agtggttcta ctttccagat gatcgccgtc atctttcgcc agataaccat
     3361 ttatcgggtg aagatgaaag gcggtagtga tgagcaagct cagagagaag ccgtcaccga
     3421 aacggcggca gcgctgggct ttatctcagc cattggcgca gtgggcggct tttttattcc
     3481 gcaggcattt ggcatgtcgc tcaatatgac cggctctccg gtcggcgcga tgaaagtgtt
     3541 tttaatcttc tacatcgttt gtgtgctgct gacctggctg gtttatggtc ggcggaagtt
     3601 cagccaaaaa taagagatcg atgcctgatg tacaaccgtg tcgcatccga caaggcatca
     3661 gatcccaatt cattcctgga gcaggagtca tgtcatgagt aaacttttgg atcgctttcg
     3721 ctacttcaaa caaaagggcg aaacctttgc cgatggtcac ggacaggtga tgcatagcaa
     3781 ccgcgactgg gaggacagct atcgccagcg ttggcagttc gacaaaatcg tgcgttccac
     3841 ccacggcgtt aactgtacag gctcctgtag ctggaaaatc tacgttaaaa atggtctggt
     3901 gacctgggaa atccaacaga ccgactaccc gcgcactcgc cctgacctgc ccaatcatga
     3961 acctcgcggc tgcccgcgtg gcgcaagtta ctcctggtat ctttacagcg ctaaccgcct
     4021 gaaatacccg ctcattcgta aacgactgat tgaactgtgg cgcgaagccc tcaaacaaca
     4081 cagcgatccg gtactggcgt gggcatcgat tatgaacgat ccgcaaaagt gcctgagcta
     4141 caaacaagtg cgtgggcgcg gcgggtttat ccgctccaac tggcaggaac taaaccagct
     4201 gattgccgcc gctaacgtct ggaccatcaa aacctacggc ccggatcgcg ttgccggttt
     4261 ctcgccgatc ccggcgatgt cgatggtttc ttacgccgcc ggaacgcgtt atctgtcgct
     4321 gcttggcggc acctgtttaa gtttctacga ctggtattgc gacctgccgc ccgcctcgcc
     4381 gatgacctgg ggcgagcaaa ccgacgtacc ggaatctgcc gactggtata actccagcta
     4441 catcatcgct tgggggtcta acgtaccgca gacacgtacg ccggacgccc acttctttac
     4501 cgaagtacgc tacaaaggca ctaaaaccat cgccattacc cctgactact ctgaagtggc
     4561 caaattgtgc gaccagtggc tggcaccgaa acaaggcact gatagcgccc tggcgatggc
     4621 aatggggcat gtgattttaa aagagtttca tctcgataat cccagcgact actttatcaa
     4681 ctactgccgc cgctacagcg acatgccgat gctggtaatg ctggagcctc gcgacgatgg
     4741 tagctacgtt cccgggcgca tgatccgcgc atctgacctg gtggatggac tgggcgaaag
     4801 caacaatccg cagtggaaaa ccgtagcagt taataccgca ggtgaattgg tagtgccgaa
     4861 cggttcgatt ggtttccgct ggggagaaaa aggcaaatgg aatctggaat ccattgccgc
     4921 cggtacggaa accgaattgt cgttaaccct gctcggtcaa catgacgctg ttgcaggcgt
     4981 ggccttcccc tactttggcg gcattgaaaa tccgcatttt cgcagcgtaa aacacaatcc
     5041 ggtgctggtg cgccaattgc ccgttaaaaa cctgacgtta gtcgatggca acacctgtcc
     5101 agtggtcagc gtttatgatt tggtactggc gaattacggc ctcgatcgcg ggctggaaga
     5161 tgaaaacagc gcgaaagatt acgctgaaat caaaccgtac accccagcct ggggtgagca
     5221 aattaccggc gtaccgcgcc agtatattga aaccatcgcc cgcgaatttg ccgataccgc
     5281 ccataaaacg catgggcgct caatgattat cctcggcgca ggtgttaacc actggtatca
     5341 catggacatg aactaccgcg ggatgatcaa tatgctgatc ttctgcggtt gtgtcgggca
     5401 aagcggtggc ggctgggcac actatgtcgg ccaggaaaaa ctgcgcccac aaaccggctg
     5461 gttgccactg gcctttgcgc tcgactggaa ccgaccaccg cgccagatga acagcacctc
     5521 gtttttctac aatcattcca gccaatggcg ctatgaaaaa gtctctgctc aggagttact
     5581 ttcaccgctc gccgatgcca gtaagtacag cggtcatctg attgatttca acgttcgcgc
     5641 cgaacgtatg ggctggctac cttctgcgcc gcagctgggg cgtaacccgc tcgggattaa
     5701 agctgaagcc gacaaggccg gattatcccc cacagaattt accgcccagg cgctgaaatc
     5761 gggcgattta cgtatggcct gcgaacaacc agatagcggc agcaatcatc cgcgtaattt
     5821 gtttgtctgg cgctctaacc tgcttggctc ctccggcaaa ggccacgagt atatgcagaa
     5881 gtatctgctg gggaccgaaa gcgggattca gggcgaggaa ctcggtgcca gcgacgggat
     5941 caaaccggaa gaagtcgagt ggcaaactgc ggcgattgaa ggcaagctcg acctgctggt
     6001 gacgctcgac ttccgcatgt ccagtacctg cctgttctcc gatatcgtcc tgcccaccgc
     6061 cacctggtat gaaaaagacg atatgaacac ctcggatatg catccattta ttcatccgct
     6121 ttctgcggcg gtcgatccgg cctgggaatc acgcagcgac tgggaaatct acaaaggtat
     6181 tgccaaagca ttttcgcaag tgtgcgtggg ccatcttggc aaagaaaccg acgtggtatt
     6241 acaaccactg ctgcatgact ctccggcaga gctctcacag ccgtgtgaag tgctcgactg
     6301 gcgcaaaggc gaatgcgatc tgatcccggg taaaaccgcg ccgaatattg tggcggtgga
     6361 gcgcgactac cctgctacgt atgaacgctt tacctcgctc gggccattga tggacaaact
     6421 tggcaatggc ggtaaaggga tttcgtggaa tacccaggat gaaatcgatt tcctcggcaa
     6481 actcaattac accaagcgtg atggcccggc acaggggcgt ccgctgattg acaccgccat
     6541 tgacgcttca gaagtgattc tggcactggc accggaaacc aacggtcatg ttgcagtcaa
     6601 agcgtggcag gcgctgggcg agatcaccgg gcgcgaacat acccatctgg cgctgcacaa
     6661 agaggacgag aagattcgct ttcgcgatat tcaggcgcag ccgcgtaaaa ttatctccag
     6721 ccccacatgg tccggtctgg aaagcgatca cgtatcctat aacgcgggat acaccaacgt
     6781 tcatgagtta attccgtggc gcacgctgtc gggacgccag cagctctatc aggatcatcc
     6841 gtggatgcgt gcttttggtg aaagcctggt ggcttatcgc ccgcctatcg acacccgtag
     6901 cgtcagtgag atgcgccaga taccgccaaa cggcttccag gaaaaagcac ttaacttcct
     6961 gacgccgcac cagaaatggg gcattcactc aacctacagt gaaaacctgc taatgctgac
     7021 gctctctcgc ggtggtcagc caacgtgtac cgccgggcat gaccatgatg tatcacgccc
     7081 aggaacgcat tatgaatatt cctggttcgg aagtaactgg catgcgcggc ggcattcata
     7141 acttggtcac ccgcgtttgc ccgaaaccga cgcatatgat tggcggttac gcgcagctgg
     7201 cctggggctt taactactac ggcaccgtcg gctcgaaccg cgacgaattc atcatgatcc
     7261 gcaagatgaa gaacgttaac tggctggatg atgaaggtcg cgatcaggta caggaggcga
     7321 aaaaatgaaa atacgttcgc aagtcggcat ggtgcttaac ctcgataaat gtatcggctg
     7381 ccatacctgt tcggtgacct gtaaaaacgt ctggaccgga cgcgaaggca tggagtacgc
     7441 atggtttaac aacgtcgaaa ccaaaccggg cattggttat ccgaaaaact gggaagatca
     7501 ggaagagtgg caaggcggct gggtccgcga tgtgaatggc aagatccgtc cgcgtctggg
     7561 cagcaagatg ggcgtaataa ccaaaatctt cgccaacccg gtggtgccgc agcagattga
     7621 tgattactac gaacctttca ccttcgacta cgaacatttg catagcacac cggaaggcaa
     7681 acatatccct actgctcgcc cgcgttcact gattgacggc aaacggatgg acaaagtgat
     7741 ctggggccca aactgggaag aactgctggg cggcgagttt gaaaaacgtg cccgcgaccg
     7801 caacttcgag gccatgcaaa aggagatgta cgggcagttt gaaaacacct tcatgatgta
     7861 cctgccgcgc ctgtgcgaac actgcctcaa tcccagctgc gtggcgacct gcccaagcgg
     7921 cgctatctac aaacgcgaag aagacggcat tgtgctgatt gatcaggata aatgccgtgg
     7981 ctggcgtttg tgcattagcg gttgtccgta caaaaaaatc tacttcaact ggaaaagcgg
     8041 caagtcagaa aaatatatct tctgttatcc gcgaattgag tcaggccaac ccaccgtgtg
     8101 ctcagaaacc tgcgtgggtc gcatccggta tctgggcgtg ctgctttacg acgccgaccg
     8161 catcgaggaa gcggcgagca ccgagcgcga agttgacctc tatc
//
LOCUS       DAETPZ010000139         8196 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.140, whole genome shotgun sequence.
ACCESSION   DAETPZ010000139 DAETPZ010000000
VERSION     DAETPZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8196
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.140"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..671)
                     /gene="chbR"
                     /locus_tag="K0W59_003738"
     CDS             complement(<1..671)
                     /gene="chbR"
                     /locus_tag="K0W59_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HBI3024588.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTDSISVLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIIN"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="K0W59_003739"
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="K0W59_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HBI3024589.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1088..2437)
                     /gene="chbC"
                     /locus_tag="K0W59_003740"
     CDS             complement(1088..2437)
                     /gene="chbC"
                     /locus_tag="K0W59_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HBI3024590.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPDFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANP"
     gene            complement(2522..2842)
                     /gene="chbB"
                     /locus_tag="K0W59_003741"
     CDS             complement(2522..2842)
                     /gene="chbB"
                     /locus_tag="K0W59_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HBI3024591.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3141..3479)
                     /gene="osmE"
                     /locus_tag="K0W59_003742"
     CDS             complement(3141..3479)
                     /gene="osmE"
                     /locus_tag="K0W59_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HBI3024592.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3681..4508
                     /gene="nadE"
                     /locus_tag="K0W59_003743"
     CDS             3681..4508
                     /gene="nadE"
                     /locus_tag="K0W59_003743"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBI3024593.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4738..5625
                     /gene="cho"
                     /locus_tag="K0W59_003744"
     CDS             4738..5625
                     /gene="cho"
                     /locus_tag="K0W59_003744"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HBI3024594.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5585..6160)
                     /gene="ves"
                     /locus_tag="K0W59_003745"
     CDS             complement(5585..6160)
                     /gene="ves"
                     /locus_tag="K0W59_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HBI3024595.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLWLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6363..6848)
                     /gene="spy"
                     /locus_tag="K0W59_003746"
     CDS             complement(6363..6848)
                     /gene="spy"
                     /locus_tag="K0W59_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HBI3024596.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7178..8146)
                     /gene="astE"
                     /locus_tag="K0W59_003747"
     CDS             complement(7178..8146)
                     /gene="astE"
                     /locus_tag="K0W59_003747"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HBI3024597.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHSEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         2154 a         2055 c         1890 g         2097 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactgcg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtac gctgatacta tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttcca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     1441 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcaatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagt
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     2161 ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     2221 tggttgaggc gtcgaggcga atacctaagg aataaaaaaa cgacccctcc ccaaagctta
     2281 gaaaaacgtt gttaattaat acaaacatgg cccccgcaag ggttaacggc attaagcgaa
     2341 taaagccatt tttgattgca ttaacgtgtg gctgctttcc tattttaact gcaaaaggga
     2401 ggagtacctt ttcaagcgat gcaataacat tactcataga aaaataccct taaaaaccgc
     2461 aatttaaata ttgcggtttt gatttatgaa ataactcttt gacgggaaaa tttaaaataa
     2521 attaatttgc tgcggctttt ttaatcgctg caaccgcagc cttaagcacg cctaaaccat
     2581 cgactttgcc ataaagcagc gagtcaatta cttcaaccgg tttgttgggt aacaaacgct
     2641 ggatttcggg caacatataa gcaatctgcg gccctaataa cacgacatcg gcattctgac
     2701 ctttttcacc agccagtgtt tccggaaatg cttcaataat gaccggaact tcatattttt
     2761 ctgcctgtgc gcgcattttt gataccagta aagagataga catgcccgca gaacaaaaca
     2821 gataaatgtg tttcttttcc ataaaactgc cctcatcgac gattatctgt cagccagaca
     2881 ctccgcaagc cttaacctgc ttccatgctc tgggtaactt gcgaaaccaa catgactttt
     2941 tttgtggctg aaaggagtat acggtataga cctgagcaat agtatttctt tgaccttata
     3001 aattgtttct ccttgacctg tggttatgac cctcttcaca tttcgggcaa ataattcgcg
     3061 aagataatta agaactcagg ccaaaaaaaa ccggcgcaat ggccggtttc cgttgttact
     3121 caagctttca gacgaattga ttacttcgca gcctgtggat cagtgtcgta ttcagcacag
     3181 gtctgataac cggagttgat gacatgtccg gtatcatcta acgcgacaaa gtaggtttct
     3241 gctttaccat cacgttgacc caggatgtag gtctggcaag taccgcgagc atggatcatg
     3301 ctcacttcag acgaaggttt acccgcaatt tgtgcaacct gcgcccggct catgcctttt
     3361 ttgacgtctt tcaccacagg ctgtacaaac tggtctttgg tacgatcata agctgtacaa
     3421 cccgccagca tggttaatac cgccgctgca ctcagaattc ctgccatatt cttgttcata
     3481 ttccgtcctc ttgtttatca gcgtgttaga taagcctgga atacattggg cgctttttca
     3541 agcccgtgaa cgaaacggct ccgctttcag aggattcctg tatgacgttt taaccaccat
     3601 tcagcccgct gtcgcttgtc gtttcagtag caacgggtta gctttaagga agttttgtct
     3661 tttctgtctg gaggggttca atgacattgc aacaacaaat aataaaggcg ctgggcgcaa
     3721 aaccgcagat taatgctgaa gaggaaattc gtcgtagtgt cgattttctg aaaagctacc
     3781 tgcaaactta tccgttcatt aaatcactgg tgctcgggat cagcggcggt caggactcca
     3841 cgcttgccgg aaagctgtgc cagatggcga ttaatgagct gcgccaggaa actggcaacg
     3901 aatcactgca atttattgcc gtacgcctgc cctatggtgt tcaggctgac gaacaagatt
     3961 gccaggatgc cattgccttt attcaaccgg atcgcgtatt aaccgttaat atcaagggcg
     4021 cggtattggc tagcgagcag gcattgcggg aagcaggcat tgaactgagc gattttgtcc
     4081 gtggcaatga aaaagcgcgt gagcggatga aagcacaata tagcattgcg ggtatgacca
     4141 gcggtgtcgt ggtgggcacc gatcatgcag cagaagccat taccggattc ttcactaaat
     4201 atggtgacgg cggtacggat attaatccgc tgtatcgtct caacaaacgc cagggtaaac
     4261 agttactgac ggcattaggt tgcccggaac acctttataa gaaagcccca actgccgatc
     4321 ttgaagatga tcgcccttct ctgccagatg aagtggcact cggcgtgacc tatgacaata
     4381 tcgacgacta tctggaaggg aaaaacctac ctgaacaggt cgccagaaca atagagaact
     4441 ggtatctgaa aaccgaacat aaacgccgtc cgccaattaa cgttttcgat gatttctgga
     4501 aaaagtaata atttgcacct attggattgt gcgaaaaaga gtaatttgtt cacgccggat
     4561 gcggcgtgaa cgccttattc gacctacaaa actatgcaaa ttcaatacat tgcaggagtc
     4621 gaataggcct gacaggcgta gcacgtcaga cggtgtaacc tttgtcatca acccgcctct
     4681 tttttaatcg cctcccgcct gttacactgg atagataacc agcattcgga gtcaacagtg
     4741 gtacggcgtt taacttctcc gcggctcgaa tttgaagctg cggcaattta tgaatatccc
     4801 gaacatttac attcattcct taatgactta cccacccgac caggggtgta tctgtttcat
     4861 ggcgaaagtg acaccatgcc gctctatatc ggcaaaagcg ttaatatccg cagtcgggtg
     4921 ctttctcatt tacgcacccc ggatgaagcc gccatgctac ggcaatcccg gcgaattagc
     4981 tggatctgca ccgcaggcga aatcggcgct ctgctccttg aagcgcgatt aatcaaagaa
     5041 caacagccgc tgtttaataa acggttgcgc cgcaatcgcc agctctgtgc cctgcaatta
     5101 aatgaaaagc gcgtcgatgt ggtgtatgcc aaagaggtgg atttttcacg agcccccaac
     5161 ctgtttggcc tgtttgccaa taggcgcgca gctttgcaag cattgcagag catcgctgat
     5221 gaacaaaaac tttgttatgg cctgttggga ctggaaccgt taagtcgcgg tcgtgcatgt
     5281 tttcgttcag cgctaaaacg ttgcgccgga gcatgctgcg gtaaagagag ccatgaggaa
     5341 catgcgctac gcttgcgcca gtctctggag cgtttgcggg tggtgtgttg gccttggcaa
     5401 ggtgcggtgg cactgaaaga acagcacccg gaaatgactc aatatcatat tattcaaaac
     5461 tggctgtggc tgggggcggt taattcgctg gaagaagcga caacgttaat tcggacaccc
     5521 gccgggtttg atcacgacgg ttataaaatt ctttgtaagc cgctgctttc cggtaactat
     5581 gaaattactg aacttgatcc ggcgaatgac cagcgagcca gttgatttca ctgaacaaca
     5641 gtttaccttg cggttgtaaa agccacaggg tgtgcctgcc atcgaaccag cacgcgccct
     5701 ggtcggtagt gagcagtttg tcgcccagtt gccacgcccc gttaatcaca aacaccaccc
     5761 cgccgcgtga gccaaaggta gtaaacgtac gttcggcaat tctgactttc gccttacaga
     5821 catccaggcg cgtcataata ttgaaatcca tcgacatctg ccctgccgtc agtttcgctt
     5881 ttaccacctg gtccgccgta aaggcaaaag gctgtaacgg ttttaaggta tggttaaagc
     5941 ggtctgcgct ttcaaggagc atctcgccgc cttccagcaa cgtcactatc ctttccatgc
     6001 cgggaaacaa agaaaactcc ccattcgccg cgatggatgc aatgctggca cgccagtaaa
     6061 aatcacgttt tgccggtggg aaagtgcaaa tttcgcgcgt ttcaccggca gcgtttcgcc
     6121 acagattcac cgacatttta cgcatatcaa agtattccat actcgctccc ggcccgttct
     6181 tttcattgtt atcgttcagg taaccgtgga tggttatcgg caactcgggt gatttacgtt
     6241 agtggtgatc aggctttttt tacaactgtt tgagaagaga aaagaaaacc gccgatcctg
     6301 tccaccgcat tactgcaagg tagtggacaa gaccggcggt cttaagtttt ttggctgaaa
     6361 gattattcag cagttgcagg cattttacct tttgccgctg gacgttctgt cagacgcttc
     6421 tcaaaattag cattaaattg ctttttctgt tccggcgtca ggatgttgta aattttgttc
     6481 tgggtttcca tgtgcgccag catgttagct ttgcgctgtt cttccatttt tgcgatctgc
     6541 gcttcagctt ttgctttatc gaaggtatcg ctggcaatga tgtcatgcat tgcgcggcgt
     6601 tcttccagcg gcggacgttt catctggtca cgctggcctt tcatgatttc gtggatctgc
     6661 tgtttctgcg cgtcggtcag gttcaggtct ttgaacatca tgtcctgatg cggaccgaac
     6721 ttgcctttgt ggtgcatcat cggcttcgcg tcagccggtg ctgcggtagt ggtgtctgcg
     6781 gcatgggcca ggttagccgc gccaagagcc agggtagagg caacaaacag tgcagttaat
     6841 ttacgcatat tctatatcct tcctttcagt tatttattac ggctttctta agtagcgtgc
     6901 cgtgttgacg agattaactt tactgacttt agcgtcaatt aatcagagca acggtaaaac
     6961 aatgaaagtg taaaaaacac ttttgcgcca attatggaga aaaaaagaaa atttgatgga
     7021 gagtgatgag agaatattac aacacgatga ttttgcagag attatgaaga actataccgg
     7081 atgactggtg ataaataaag caaataacca ggattaatct gtattaattt ataagaaagc
     7141 aacttaatac ccgcagaatg atttctgcgg gtaagtatta gcttattttt tcgagcatta
     7201 atcccgcgcg taatcccaac gctaccaacg gattagggaa taacacatac tctacatcat
     7261 gggttacggt aaaacgttcc tctccgtcct gcgccagcaa tgttcctttc tcaaacggca
     7321 taaaattcag cgtgtcactt gccatatgca tttcgaagga cggcgagtgg cgagtaattt
     7381 gcgaaaccac ccgataacgg agcggcggtg ttctcacgat accgacactc tcaccagata
     7441 gcagcgcagc aattgcgctg gcagttactg caaactggcg aagatcgttt tgcccaaagg
     7501 gcaacgcttt gccaagttcc aacgtacagg ccagcgcgcc aaaatgtctg acgctgaaat
     7561 gggtaaacgt accaccaggc tcctgatgga acaccagcgc ctccagcccc gccgcaccca
     7621 gccacgtcag aaatttctcg tcccagggaa tgtcgcgttg cggtaataca ccgaactgcg
     7681 gatgcaagga gccacgaatt gcggtatgta gatcaaggtg ccaacgcaca gattctttgc
     7741 cctggtcata aaaatcttcc aggcactgtt ccagttcgcg cgcccgacag gtttctccgc
     7801 tttcagcaaa tagctgccaa cgaccgccaa acattcggtt catatcgcta tggcaataac
     7861 gtttcccttg cttcagcgca ggaggattcc cgaggatcac cagcaaccgc caacgtaacg
     7921 ggatctcgct gtgagatatc gcgccaagca acgcgtccag catttccaca ggtgccgtct
     7981 cattaccgtg gatccccgca gaaatcacca gcgcgccttg cggtggcgtt aatggcgtca
     8041 gttccagcac accatcgccc agccagcgcc agcgaacgcc gttgatttct cgctcggtga
     8101 taaccggttt tttacccgtt aaggtcagag caagaaaatt atccattgcc ccctccctcg
     8161 cgctggaacg gataaaccga accgagattc agtaat
//
LOCUS       DAETPZ010000140         8158 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.141, whole genome shotgun sequence.
ACCESSION   DAETPZ010000140 DAETPZ010000000
VERSION     DAETPZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.141"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(61..600)
                     /locus_tag="K0W59_003748"
     CDS             complement(61..600)
                     /locus_tag="K0W59_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBI3024598.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(665..1213)
                     /gene="fimA"
                     /locus_tag="K0W59_003749"
     CDS             complement(665..1213)
                     /gene="fimA"
                     /locus_tag="K0W59_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBI3024599.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTINFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(1694..2290)
                     /gene="fimE"
                     /locus_tag="K0W59_003750"
     CDS             complement(1694..2290)
                     /gene="fimE"
                     /locus_tag="K0W59_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HBI3024600.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(2768..3370)
                     /gene="fimB"
                     /locus_tag="K0W59_003751"
                     /pseudo
     CDS             complement(2768..3370)
                     /gene="fimB"
                     /locus_tag="K0W59_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            4824..5540
                     /gene="nanC"
                     /locus_tag="K0W59_003752"
     CDS             4824..5540
                     /gene="nanC"
                     /locus_tag="K0W59_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HBI3024601.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            5560..6666
                     /gene="nanM"
                     /locus_tag="K0W59_003753"
     CDS             5560..6666
                     /gene="nanM"
                     /locus_tag="K0W59_003753"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HBI3024602.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            6731..7711
                     /locus_tag="K0W59_003754"
     CDS             6731..7711
                     /locus_tag="K0W59_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBI3024603.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTTLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2425 a         1743 c         1699 g         2291 t
ORIGIN      
        1 tacattgacg tttttattac tcactgtcct gttcctgtta tcacattatc tactgaacaa
       61 ttactgatag gttaaagaga accaggcctg ggcattggcg atgccgccag taacccgacg
      121 cccggtagca cgatatttgg cgatgaaatg tagcgaagta gagcctgagt aaagccgttt
      181 ccagtttgct ggaggacgat taatcggtac gaggtttcct tcatcatcaa acaacgctac
      241 accaatattg gaggctatcc ctggcccctc tcccacggag agcacatccg gatttttacc
      301 atccgcgaca ccgtgaaacg ccacacccac acgttcactc accaccgtgc tacattcccg
      361 taaatgaata acaaaaggca ccggtgcgct atcttcccct gccgcatgaa accggttact
      421 gctgatttgc cccatattga ccgtcatttg tttatcaccg gcttcaatcc ggcaagtttc
      481 cgcaataatg acgccctgaa attgcatatt tccgccgggc agcgtggtat tccatttatt
      541 tccggccaga gcaaacattg gcagcaacga cgctaacaga aataatctct tccttttcat
      601 cgttattttt atcgcacaag ggtgggcatc cctgcccgta atgacgtcct tgaacctggg
      661 taggttattg atactgaacc ttgaaggttg catccgcatt agctgcaccc ggggttgcct
      721 cgccgattgc ataataacgc gcctggaacg gaatggtgtt ggtaccgtta ttcagggttg
      781 tttgtgcact aaatgtcgca ccgtccagcg tcagagcatt gcctgttctg tccaggatct
      841 gcacgccaac gtttgttgca ctacctgcag cagaactctg cagagctagt acatcagtac
      901 gcgtagcatc aattgccgta cctaagaagg caacagcggc ttttgtggca acagtggtat
      961 cgcaatcatt cagctgaatg ttaaaaccaa cggcagagct ggttgctcca gcctgcttca
     1021 ggctagcggt acgaacctgt cccaactgaa cggtttgatc aacagagcct gcatcaactg
     1081 cgcaagcggc gttaacaact tcccctttaa agttaatggt cccaccattt accgtcgtag
     1141 tatcggccag agccgctgcg gaactgaggg acagagccga cagaacaacg attgccagag
     1201 ttttaatttt catgctgctt tcctttaaaa aaactatttc taaatcgaca tgggcagtcg
     1261 ttctgtacac tttgttttgt caacgagttt gactgccaac actgcacagt tttcccccaa
     1321 aagatgaaac atttggggcc attttgactc atagaggaaa gcatcgcgca caaacttttt
     1381 cagtttattt gttggcttaa tattctattg ttatctttat ttatagattt ttatattgca
     1441 tgaggtggtt tttggagaga agaatgagga agatgcgtcg agccacagaa acgttagctt
     1501 tacatatagc ggaggtgatg tgaaattaat ttacaagaga aataatttac atatcaaata
     1561 gttagatgct ttttgtcgtt ttttaatatt tttatgcttg agaaaaaata agtaacttat
     1621 ttatgatatg gacagtttgg ccccaattgt cttgtgttta tttgtttttt aactttatta
     1681 tcaataagtt aaatcaaacc tcttctcttt ttaatttttc gtttatgaga ttatttcttt
     1741 cccataatcc ggcaaaacga gcagcattac tggcggtata acgcacagta tggcgaatat
     1801 ttcgatgccc gagataatcc tgaattaaac gggtatctgc accacgctcc gccagttcat
     1861 aaccgcaagc atgccttaac atatgaggat gagtctgcgt tacggttcca gcttcaatac
     1921 cggcatcgcg aataatgcga taggcctgct ggcgagaaag ccgactcccg cggcgagaaa
     1981 taaatatagc gtcggtccgg tcagcgcctt tccagttagc acgttcctgg gtccagcgtt
     2041 ccacggcttc acgctcatca aaacgtaacg ggtgaacggt agaaaatccg ttcttcagtc
     2101 ggcgaatatt tattcgacct tcattaaggt caaggtcctg ataatgcaga tcaagcagtt
     2161 cactaatacg catcccatgc cgatatgcca acagaataag acaataatct ctggctcccg
     2221 ttgccccgta acaaaccgcc tgcatcatgg cctgaacttc tttaccggta agataacgac
     2281 gtttactcac aatagtagta ctcctaactg agatatattt gaatgaatac ctataggaaa
     2341 cctcaatcgg tcaaaattag cccaatagat aaaaagataa ccacacacga agttatgtgg
     2401 ctatcagtca attacattaa acatcataca aaaataaaat atcactgatg tgacaacatc
     2461 tattttcatt gatttgattc aatataacaa tagattacag aggattaact tccagttact
     2521 taaaaaataa agaaaaaagt gtggaaacag gagaatttaa gtgatgtgta aacttatgaa
     2581 tgattttgtt tatttatttt gaagaacata aaccattaga ttttgtatgg gtatggttaa
     2641 cttaatgttt attatgataa ttaagaatca cacttacaat aacatcaatt aataaatggt
     2701 taatcatttt tgatatatcg taagaataat gtagttttta acaccatccc tggtatctca
     2761 actatctcta taaaacagca tgacgctgtc gtcctctggc tctatcccag atgccgtaaa
     2821 aacgccctgc attgctggcg gtataccaga cagtatgacg aatattgcga tgcccaagat
     2881 aatcctagat aagtcgcgta tctattccta tattcgccaa agcaaaacca cacgaatagc
     2941 gtaacatgtg cggatgaatc tccagtgaca acccggcatt accaccagaa gtcgagataa
     3001 tatggtaaaa ctgttgccga gaaagcggat tccccttacg tgataaaaat acccactcgc
     3061 tctcagcatg cggataagaa gtacggatac tcaaccagtt ttttaaagcc tgaacttctt
     3121 tgttcaatag cgggtgcgtt gttgaaaagc ctttttttaa tcgatgtata tatatacact
     3181 ttgctttaag atcaatatcc gaaatcctca atcgacaaat ttcactcgcc cggaaaccat
     3241 gaataaaaca aagcaaaatc agacaataat tacgtgttgc atgaggcccg gtatttgctg
     3301 ctttaaggag tgactcgatt tcactatggg taaggaagtt cctttttttg ttatcagcct
     3361 tattcctcat cgttttccct tataattaca gacgcgcact agctgtgctg ggttagcaat
     3421 aatccaacat ttttatcctg attatgttta taaaagcgaa cgtttgctta ataacttacg
     3481 taagtggacc agttcttctg agcgaactta aatggagtag cgatagttac ttataataac
     3541 tttgccatac aaacgccatg ctcttgcatg ctatgtacct ttatatattt atcaatctga
     3601 tacgaatcta ataataaccc tcatcattaa tgcaactaat cttatctata tatcatgtga
     3661 tatgttctgt aacaagtaaa atcaacataa aaaattcagc aaaatttaat atttgttcaa
     3721 ctagccgtct caaatatgac attattagtt aactgcattt gccacaaaat ggctatttca
     3781 tggaacgcag taatttctta gtggctatta tcatgctaat tcttagatcc ctagcgatta
     3841 ttcctgcctg gttcgtcagc ttaggccatg agattgttac attccttttt atcctggaga
     3901 tatgggggaa cgcaggcgac gctttttcat ctacatccgt tggtaaatat agctttggtc
     3961 actacgactt aagccttcct caatcacagc tattcgccgg gcgatacggc aaacaaacta
     4021 agtgaaacat catcctgaaa acaccaacat caacaagcct ctccagatcg acatcaggag
     4081 tgaccagtta caagccacaa accaagaaac atatttcatg atgagaatta tgctcagcct
     4141 gatggcgagt aaacccttat tttcaccact ctcactgagt tagcttgcaa atctttcttt
     4201 gtttagattc taaataaatt atttataaaa catatagtta aaagaaaatc tttagttttt
     4261 gaagaccacc gcaagtgttc gtctggcttc acatggcatc ttcttcttag aaaaagatcg
     4321 acatattttg tgacgcgaat tgcaaatctg gttttgttgt atggattgcg tgatttttga
     4381 tctggtataa caggtataaa gatgcaccac gatagtcaat gagactgggc atctcgtaat
     4441 ctatggcaaa catcacttca gttctttcac atcgggtgat gaaaatgcac ttcagtctga
     4501 aaggaatatg aaaatgagat caacagacac tctattttat gactctgggt aaaatgggtt
     4561 gagtaagtga tataacttac gaatattcaa atcaattaaa aatcagaaga gattttatac
     4621 tcaggtattt aatctggatc tctgtttatt taaataatgt taaaagagaa ttttcacagg
     4681 agaccttata caaaaaatat aaaatacagt tacccgttgc caaagacact ataagcctgg
     4741 caaaaaaata ttacacaaca taaatgctaa ttgtttatgc gggctttgta ttgctttctg
     4801 tatcctacaa atgagtgaaa tttatgaaaa aggctaaaat actttctggc gtattattac
     4861 tgtgcttttc gtccccatta atttctcagg ctgcgacact ggacgtacgt ggtggatatc
     4921 gtagtggaag ccacgcctat gagactcgac tcaaagtcag tgagggatgg caaaatggat
     4981 ggtgggcaag catggaaagt aatacctgga ataccattca tgataataaa aaggaaaatg
     5041 ccgcactcaa tgatgttcag gttgaagtta attacgcaat taaacttgat gatcaatgga
     5101 cggtgcgccc gggaatgtta acgcatttta gcagcaacgg cacacgctac ggaccctacg
     5161 taaaactgtc ctgggacgcg acaaaagatc ttaattttgg cattcgctat cgttacgact
     5221 ggaaagctta ccgacaacaa gacttatccg gtgatatgtc tcgtgataac gttcatcgtt
     5281 gggatggata tgtcacttac catattaata gtgatttcac cttcgcatgg caaacgacgc
     5341 tatacagcaa acagaatgat tatcgctatg caaaccacaa gaaatgggcg acggaaaatg
     5401 cctttgttct acaataccat atgacgcccg atattacgcc atacatagaa tatgactacc
     5461 ttgaccgcca gggtgtttac aacggcagag ataatttatc ggaaaacagt tatcgcattg
     5521 gtgtgtcatt taaactgtag tagacaggag acagtcacaa tgaataaaac tataatggcg
     5581 cttgctatca tgatggcttc atttgccgca aacgcgtctg tattaccgga aactcctgtg
     5641 ccatttaaaa gtggtaccgg agcaattgat aacgacactg tctacattgg tttaggtagc
     5701 gcaggtacag catggtacaa gctagataca caggccaaag ataaaaaatg gactgcgtta
     5761 gctgcattcc ctggtggacc gagagagcaa gcaacctctg catttattga tggcaatctg
     5821 tatgtgtttg gcggcattgg caaaaacagc gagggattga ctcaggtatt taatgacgta
     5881 cacaaataca accccaaaac caatagctgg gttaaattga tgtcgcacgc gccgatgggc
     5941 atggcgggtc atgtgacttt tgtacacaac ggcaaggctt atgttactgg cggtgttaac
     6001 cagaatatct tcaatggcta ttttgaagat ctcaacgagg ctggaaaaga ttcaaccgct
     6061 atagataaaa tcaacgccca ctattttgac aaaaaagcag aagattattt cttcaataag
     6121 tttctgttgt cttttgatcc ctcaacacag caatggagtt acgctggcga atcgccctgg
     6181 tacggaacgg ctggtgcggc ggttgtgaat aaaggtgata aaacctggct tattaatggc
     6241 gaagccaaac caggattgcg aacggatgcc gtatttgaac ttgatttcac cggtaataat
     6301 ttaaaatgga ataagcttga tcccgtctca tcaccagatg gcgtcgctgg cggttttgcg
     6361 gggataagca atgattctct tatatttgcc ggaggggccg gattcaaagg ttcacgagaa
     6421 aattaccaga acggtaagaa ctatgcgcat gaaggcctga aaaaatcata tagcactgat
     6481 attcatcttt ggcataacgg gaaatgggat aaatcgggtg aattatcgca aggtcgggcc
     6541 tacggagtat cattgccctg gaataatagt ttattaatta ttggcggtga aactgcaggc
     6601 ggcaaagcgg tgacggattc agttttaatt tctgtgaagg ataataaagt cacagtacaa
     6661 aactaacgct tcagggcccc ggtaaagggg ccctgctatc aatttgtcat ttgtattacg
     6721 cgaaaattat atgaacgcaa taatatcgcc cgattattac tatgttctta ccgttgctgg
     6781 tcagtctaat gccatggcgt atggcgaagg actgccatta ccggacaggg aagatgcgcc
     6841 tcattccaga attaaacaat tagcgagatt tgcgcatacg catcccggag gcccgccatg
     6901 tcactttaac gacattattc cactgactca ctgcccacac gatgttcagg atatgcaggg
     6961 ttatcaccat cctctggcaa cgaatcatca aacacagtac ggcaccgttg gccaggcact
     7021 gcatattgca cggaaattac tgcccttcat tcctgataat gcagggattc ttatcgttcc
     7081 gtgttgccgt ggcggatcgg cttttaccgc gggcagcgaa gggacatatt cagaacggca
     7141 cggagccagc catgatgctt gtcgttgggg aacggatact acgctgtacc aggatttagt
     7201 cagcagaacg cgagccgcac tggcaaaaaa tccgcagaac aaattcctcg gcgtatgctg
     7261 gatgcaaggc gaatttgact taatgaccag tgactacgcg tcacaccctc aacactttaa
     7321 tcatatggtt gaagcctttc gtagggatct aaaacaatac cattctcagc ttaataatat
     7381 tactgacgcg ccgtggtttt gcggcgatac cacctggtac tggaaagaaa atttcccgca
     7441 ttcgtatgaa gctatttatg gcaattatca aaataatgtt ttagccaata ttattttcgt
     7501 cgacttccag caacaaggtg aaagaggact gacgaacgcg cctgatgaag acccggacga
     7561 tttaagcacg ggatattacg gttcagcgta ccggtcaccg gagaactgga cgacggcact
     7621 gcgaagcagt catttcagca cggcagcccg tcgggggatt atttctgaca gatttgtaga
     7681 agcaattttg cagttttggc gcgaaaggtg agcggggagt ttattcatgg gggatgtcct
     7741 tgttcgttct cagagtagac aacaaaaaac aaatgagatc aaggtaatat ctcttcactt
     7801 gcgggctcga acagcaggca attgccaata taataatctg gcacaagggt gaaaaacgcg
     7861 caccgcataa accgttgtta ttgcttacag attagccgga tgccttaata gacatccgcg
     7921 ccttttcgat tatggctcgg aaatctacga acatctacac agtttactgg aacgttttgg
     7981 gccacagcgt tcgcagcacc gtcctgatat gccgttctgg cgattataag tcgacggatt
     8041 ctggcaaata gacgatgatg atatgcttaa tgatgttcaa agaaattagg ccattattat
     8101 attattgctt gcattttctt tcattcctaa aagcacccgc cattaatccc aggcgcat
//
LOCUS       DAETPZ010000141         8080 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.142, whole genome shotgun sequence.
ACCESSION   DAETPZ010000141 DAETPZ010000000
VERSION     DAETPZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.142"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..1242)
                     /locus_tag="K0W59_003755"
     CDS             complement(<1..1242)
                     /locus_tag="K0W59_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBI3024604.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSL"
     gene            complement(1239..2117)
                     /locus_tag="K0W59_003756"
     CDS             complement(1239..2117)
                     /locus_tag="K0W59_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBI3024605.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(2128..3035)
                     /locus_tag="K0W59_003757"
                     /pseudo
     CDS             complement(2128..3035)
                     /locus_tag="K0W59_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(3022..3255)
                     /locus_tag="K0W59_003758"
     CDS             complement(3022..3255)
                     /locus_tag="K0W59_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024606.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(3252..4109)
                     /locus_tag="K0W59_003759"
     CDS             complement(3252..4109)
                     /locus_tag="K0W59_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBI3024607.1"
                     /translation="MSAQYIDSAISGRYTVSAPYKAGAGIGVLKFNIEHNRAHAVFSC
                     HEHCYAQIMVGRAGASQDAPGSMLTGYANPVRLTTSVIGVPCGEFFEFNIGAVTMTTL
                     PTLSQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRILDCSPEFCARNFAETSILV
                     RQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNQMERSLSGAGAADM
                     SSVAQNARGVYLHLREIHQIWTSQLYPMLKAVESPLAGKLYDRVGDAVFGAALVDSRL
                     NGSDKEVLP"
     gene            4134..4463
                     /locus_tag="K0W59_003760"
     CDS             4134..4463
                     /locus_tag="K0W59_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBI3024608.1"
                     /translation="MNYTIEYYSEEVRLEVDQLPLSMRARYQHLVERMKVYGSNLGEP
                     HTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKTQKTPPAERKKAETRM
                     KEVKHDW"
     gene            4453..4761
                     /locus_tag="K0W59_003761"
     CDS             4453..4761
                     /locus_tag="K0W59_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3024609.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(4803..4979)
                     /locus_tag="K0W59_003762"
     CDS             complement(4803..4979)
                     /locus_tag="K0W59_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HBI3024610.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(4976..5386)
                     /locus_tag="K0W59_003763"
     CDS             complement(4976..5386)
                     /locus_tag="K0W59_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HBI3024611.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(5383..5661)
                     /locus_tag="K0W59_003764"
     CDS             complement(5383..5661)
                     /locus_tag="K0W59_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024612.1"
                     /translation="MNDSYRQFENWWSKDESQFTGDDELKEFAWVIWQAPRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            complement(5736..>8080)
                     /locus_tag="K0W59_003765"
     CDS             complement(5736..>8080)
                     /locus_tag="K0W59_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBI3024613.1"
                     /translation="GDCEPCLFGWQALDAGVRAVVLCEGEIDCMSYAQYGISALSVPF
                     GGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIASRLGEHRCRLVTLPYK
                     DINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAFYGKQQYLFNPPWES
                     LADKFQFREAELTLVNGVNGHGKACPLNEPILLADGTWTTHGNVKIGDQVASVDGNPS
                     TVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEKRRVIDTFGLKRLSE
                     TKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKFSNVEPYMIERMKAE
                     LPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFIPRVFFSANKSTRIG
                     MLCGLLETDGYVEKDGTLVFSSASEELRNGVVQLVNSLGGSCRTRVKTGVTYTYKDDK
                     RHGMDSYEARIRLTREIREAIRSPRLNGRLTAHRFEGCGVFVRNVEKIGNAECLCIMV
                     DHPRHLYVTRGYVATHNTEVVGHMALEAMRQGVKTCIASLELKPGILLKRLTRQATCC
                     KMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFDYARRRYGIQLFIIDSLMKCG
                     IGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDSEEKPTGKMDVKGSGAITDLT
                     DNLFIIWRNKARERALQRVQSGEKMSEKDEQLLASPASVLMLEKQRNGEGWEGGVPLF
                     LDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVTEY"
BASE COUNT         1919 a         2203 c         1854 g         2104 t
ORIGIN      
        1 cagagaaccg ttacgttgtt tcgtgatatt gatttctgcg atgcccctgg cctgcgtatc
       61 cgggttgtac acttcatccc tgtaaagcat cagaatgatg tctgcatccg cctctatttc
      121 tccggaattt ttcaggtctg agttcatggg acgtttattg ggtctggact ccacaccgcg
      181 ggagagctgg ctcagcgcaa tcaacggaaa accaccggat tttgccaggc ctttaagccc
      241 ctttgagatt tcacccacgg caaggtcatg acgccccgtg gttcgggttt ttatcagccc
      301 gagataatca accaccacca gcgccgtttc cggatattta atcaggtggt gtttcgttgt
      361 tgcgcatatc tcatcaatgg ccaggttcgc ctggtccacc atccagatat tgcgcccggt
      421 catccgcccc accccttgtg agaaacgcgt ccagtcttca tcttcaaagt gagccacaga
      481 tttcaggcgt gatactggca tccctccagc cgcagacacc atacgttcac caatctggat
      541 gttcgccatc tccatggtga acagaagcac accatgcccc tgctcagtca ccttgtcgat
      601 gatgtccagc gcaagttcgg ttttccccat cgaaggacgg gcggcaatga ataccaggtc
      661 tccgggctcc ataccgcctg tttttgcgtc cagttcatca ataccggtca tcaacgtcct
      721 ggatttctcc agcccctgat tccggcattc aacacgctca accacttccg gaagcacatc
      781 atcaatatgt accggctgaa tgacgccctt tcctgtcgac agtgtgacca tcatgttctg
      841 cgcatccttc agggcatctt cagctgcttc acaggtatgc gcatcacgta atttctgcag
      901 cgcctcattc agtgtttttt ctgcatcgcg cagtgcggca ttgcgccgca acgctgcaac
      961 atagtgctcc agtgaagact tcacccaggt tttacgcccg gtatcagtaa tcaccggggc
     1021 aagttccggc atctcattac acaacagcac ggggtcaatc actcctgaaa cacgggcctg
     1081 tctgcagatg cctgtataga tatcctgata cgctcgtaca gaaaaaacgt ccgctggcag
     1141 tgtggccaga atatccatca cttcaggatc tgctccgcgc agaaagaacg cgccgatgac
     1201 agcgccttca aggtcatcgt tacgccatac tggagttgtc atgcagccac acctctgata
     1261 cgagaacggt aactgggcca gttaaacgac aaccagttgc gtcccccgtc tgtgatcctg
     1321 tcggcaattc ggggactgat gaacgcccac aactcttccg gtgagaggtt actgatcaga
     1381 atggtgggca agatactttc gtacctggcg ttgataattt cctgcaaaat agccatttca
     1441 gccgcgctgc caaactgaac gccaacttcg tcgatgatca gcaaatccat tgacgcataa
     1501 cgctcaataa cttcatccgc tgttttttcg ctgtcattcc gccagcaatt tttcacagca
     1561 cgggtaaggc gcatcacgtc ggtgatctcc acactggcca gatagttacg gatgatgtgt
     1621 tttgccatag ccacagccag atgatttttt ccggtaccac aactgccggt cataacaaga
     1681 ctggtaccgt tctccagcat atctggccaa ttctccgcat agcggcgaca ggccgcaaga
     1741 tttctggctg cgccaggatt aacctccaga taattatcaa actcgcagtc ccgaaaacgc
     1801 agggcaattc cggcgttatc agtcagctct tccgccttga tggacgacag ctccatggtc
     1861 aaatcgttgg cctcagcgat taagcagtca gggcagcatg aaattttttc tctgtcctcg
     1921 ccattacgat cgctccacac cagtatatgc gtgtgatatt cgccatgttt ttcgcaatat
     1981 ccgcgacctt cacgcatcag gcaggaacga taaggccatg gcttttcgcc cttctgagca
     2041 aatgcaatct ctgcccgtaa ctcatccatt cgcgcctgta gtcttttttg ttgttcacgc
     2101 aggttaaacg tcatcatcgc tgtcacctca gaatgtcagt ttgtcactgg atttaccgaa
     2161 tttgtcagac atggctccca ggccagccag gacatcgacc tgtcgctgtc gcccacctcc
     2221 gggagcggct ggctgttgcc agaagtcttc gaagtgacga tcgggtccaa agaacgtcgc
     2281 agcctgcttc acgaactgtg tgccggtatt tcctgtggca cgtacccagg cagcatagcg
     2341 tttcacgcca tcaagcatgg cttcaggttt tattccctcc ctgagccggg ctttccaggc
     2401 tttgaaggct gcggattttg agttaccacc agcacgtttt ggatattcct gccaggcctg
     2461 ttcaaattcc ggtgaatatt cctgtcggga agaacgcgct ggtgcagacg cgtcagcgga
     2521 tgcgccaata gtgtttttac tctctgtagt attctctgaa gtaatctctg ttgtattctc
     2581 tgtaagatcg aaattggttt tcccttctcc gcggcgaggg gtttcccgtg tccgcggtga
     2641 aggctttccc tcctccgcga aattgggttt tacagtttcc cgaaaacggg tttccccatt
     2701 tcgggaaact gattgttttc attgataatt tcattaaggc gctcacaatc tatacggtag
     2761 aacattttgt gctcaagacg cttgttggtt tcaaccaaaa tgcctctgga cacaagatgc
     2821 ttacgcgcta cagcctgttg ttcaaatgta agtccggttt cgtgttgtat ctcttcacgc
     2881 gttttatgta cgccttccgc tgcatgtgct ttatcctgcc agtaaaaaat ctgaccaaag
     2941 aaaataacag cgtgcggact tcccatgtat ttaacgagcc cagggtaata agcaaccgga
     3001 tgtccaaaat cgagcagaag atcagacgga cgcatagcca cctcccaggc gtttaaacat
     3061 ttttccgggc tgaaacgcca ccagcggata actcagggta tgagtacgtc cctgaacctg
     3121 gcagacaacc ttctggcttt cggtactgac caggcaaacc cgcagaacgt agccgttgct
     3181 ggtggtgaac cactgcccca cacgggggca atggttgtat cggtgataca ggaaattaac
     3241 gatgtggcgg atcatgggag cacctccttg tcagaaccat tcagcctgga atcaacaagt
     3301 gcagcaccaa aaacagcatc acctacacgg tcgtacagtt tgccagccag cggagattca
     3361 acggccttaa gcattgggta aagctggctt gtccagattt gatggatttc acgcaaatgc
     3421 aggtatacgc ctctggcgtt ttgtgcgaca gatgacatgt cagccgcacc agctcctgat
     3481 aagctcctct ccatctggtt aaaggcattg atgtatgcct ctttgaaccg ggcggcacgt
     3541 ttaccagtga agcccatagc aaggaaggcg aagccgtcgc gggtaatttg gtagcaggga
     3601 agtttgcgag taccgccgtt gggctggcgt accaaaattg atgtctccgc aaaattgcgg
     3661 gcacaaaact ctggagaaca atccaaaatg cggatctttt tcagaacatc gtcatgacgt
     3721 ttagagaaga agttggcaac agccagggat gaagtaacag cctgaccatc aacgatggca
     3781 atttcaggtt gagagagggt tgggagagta gtcatggtga cagcccctat gttgaattca
     3841 aagaactcac cacatgggac gccaatcaca gaggtggtga gacgtacagg gttggcgtaa
     3901 ccggtcaaca tagaacccgg cgcatcttgc gatgcccctg cacgccccac cataatttgg
     3961 gcgtagcaat gctcatgaca cgaaaaaacc gcatgagcgc ggttatgctc tatattgaat
     4021 ttcaggacgc caatcccggc acccgcttta taaggtgcag agacagtgta acgtcccgaa
     4081 attgcagaat caatatattg tgcagatata acgattttgt tatgttttgc tgtatgaact
     4141 acactatcga atactacagt gaagaggtcc gactggaggt cgatcagctt ccattgagta
     4201 tgcgtgccag ataccagcat cttgttgaac gcatgaaggt atacggcagc aatctcggag
     4261 aacctcacac cagcgccttt ggtgacgggc ttttcgaact cagaattaaa ggcaacgatg
     4321 ggatcgcgcg tgttttttac tgcactctga cagggaaacg catcatcatg ctgcatagtt
     4381 ttgtaaagaa aacgcagaaa acaccgccag ccgaacgcaa gaaagctgaa accagaatga
     4441 aggaggttaa gcatgactgg taaacgcact ctccccacca tgacacacga cgaaatggca
     4501 gccaaatgga tggaagaccc ggcttttaaa gcagaatacg acgctatcgc tgacgaattc
     4561 gcactgcttg atgaaatgct ggcagcacgc aaagaagctg gcttaactca ggctgaagtt
     4621 gccgagcgaa tgggaacaaa agcgaccgcg atcaccagaa tggaaagtaa tctcgcatca
     4681 ggtatcagcg gcccatcatt tgccacacta aaaaaattcg cccgcgctac tggaaaaaaa
     4741 ctccagatcc gcttcgttta accacaccgc gccgtcattc tggcggcgca ccggataaat
     4801 agtcatttcc tcgcacgatg tcttagccac cggatatccc acaggtgagc cgtgtagttg
     4861 aaggttttta cgtcagattc ttttgggatt ggcttgcgtt tatttctgga gcgtttcgtt
     4921 ggaaggtatt tgcagttttc gcagatgatg tcggtgaaac ttcgtcgctg tcgcctcatt
     4981 catacctcct gtcggtaaat ctgacaccct gaccaatagc ccaggctgtt gtgtactcga
     5041 tcagacttgc catgcgcttc acgctcatct gcgcgctgct ttcgcgaatg ttgacgcatt
     5101 ctccttcaag gccgggcaaa acatcagctt cctgttttgt tgccactgca tgaccgctga
     5161 tcaacaaaac cttccattgt tctggtttta accatttgcc gcaccattga acctgacgag
     5221 cgatatccgc cagcatcgcg tgaaattttg cgttctggtc aagatttcgc ttgtagtcag
     5281 taatgcggat ggtgactggc ttgtctttat cgagaggagt tgagaggatg gcatttattg
     5341 cggcttgctg ttgttgctta gttcggagga agattgtttg cttcatcgaa aattcttctc
     5401 tttaattcca gcggctctga tagctttcat tactgcaatt accgttttgt ccttcccatc
     5461 ctcatgcccc atcgcataag caccttcttc accatctttc caaaagtcgt cattcgattc
     5521 gggccagtcg atatccagtt caatagctgc tcgcggtgcc tgccatatca cccaggcaaa
     5581 ctcttttaat tcatcgtctc ctgtgaactg gctttcgtct tttgaccacc agttttcaaa
     5641 ctgtcggtag ctatcgttca cttccctctc ccccaaataa aaaggcctgc gattaccagc
     5701 aggcctgcta ttagctcagt gatgtagatg gtcatttaat actccgtcac gttttcctgt
     5761 cgccacgcct cgtcatattc cgatttcggc atattggcga tgtagctata tggcgatcct
     5821 gattcaagtt gcaggaactg gtgcgattgc tcgtcaagga acaacgggac accaccttcc
     5881 caaccttcgc cgttacgttg tttttcaagc atcaaaacag atgccggaga tgccagtagc
     5941 tgttcgtcct tctctgacat cttttcacca ctctgaactc tctgtaacgc tctctcgcga
     6001 gccttgttac gccagatgat gaaaaggttg tctgtcaggt ctgttatcgc tccagagcct
     6061 tttacgtcca ttttcccggt tggtttttct tcgctgtctc cttttcgcga gtgagtaacg
     6121 agaatgacgt gggagtttgt tttgtttttg aagtcgcaaa tcgagtcaac aaacgccttc
     6181 tgcccgttat agtcatcgtc gcctatgcca catttcatca ggctgtcgat gatgaataac
     6241 tggatcccgt atcggcggcg agcgtagtcg aatatttcga tcagcctgtc ggctttcgcc
     6301 gttccagtca ggccaaacac ccaaagtctt tcgtcataaa atttaaatgc agagtcaatt
     6361 tccagcactg gcggcatctt gcagcacgtt gcctgacggg taagtcgctt aaggagaata
     6421 ccaggcttca gctcaagtga cgcgatgcac gtcttcacac cctgacgcat tgcctcaagt
     6481 gccatatgcc cgacaacctc ggtgttatgc gtcgccacat atcccctggt tacatacagg
     6541 tggcgagggt gatcgaccat aatacacaag cattctgcat tgccgatttt ttcaacattc
     6601 ctgacgaata ccccacagcc ctcgaatcga tgcgcagtta atctgccatt gagtcgtggt
     6661 gaacggatgg cctccctgat ttctcttgtc agtctgattc ttgcctcgta tgaatccatc
     6721 ccatgccgct tatcgtcctt gtatgtgtat gtcacgccag ttttaactcg cgtccggcat
     6781 gacccgccga gtgagttaac cagttgaaca accccattgc gcagttcttc acttgctgag
     6841 gaaaaaacaa gcgttccatc cttctcgaca tacccatccg tttcaagcag accacacagc
     6901 atgcctatac gcgttgattt atttgcggaa aaaaacacac gagggatgaa tttgtttttt
     6961 gctgtgcacc ccattagtcc ataacctcgc agggtctcca tgagtggatt tacctgacca
     7021 cgcgccgttg atatcagcca gtccttacca tctccagaga agttgtaatc aggcagttca
     7081 gccttcatac gctcgatcat gtatggctcg acgtttgaaa acttcacgct cccattgcta
     7141 agactaccat ccccgagaag ggagccgata acccatgcta atggctctga gtggtcgcca
     7201 aagtcaccag ttatttcagg aatcctaacg ccatttttgt gcctcttcgt ttcactcaac
     7261 cgcttcaacc cgaaggtatc tatcacgcgg cgtttctcgc ctttcgtgaa tccacgacta
     7321 gtgacctccc ataggtgatc gcctgcgcaa tcaacataac gaccatcttc aaatgtgact
     7381 cggtaaacat ctctaacacc ctgcgggaat atcccagtga cagttgacgg attgccgtct
     7441 actgacgcca cctgatcgcc aatttttaca ttcccgtgag tagtccatgt cccatcagct
     7501 aaaagaatag gctcattcag tgggcatgcc tttccgtgac cgttcacacc attgaccagc
     7561 gtcaactctg cctcacggaa ctggaattta tctgccagag attcccacgg tggattaaac
     7621 agatactgct gcttgccgta gaaagcgttg atagtgtcct ggtaaaactc tcgcgcactg
     7681 tagagttctt caggatcgaa gtaggatgcc gtgccgatgt actgccagat ttcatcctcg
     7741 gtaacaccgt tcatcaggca ttcgttgatg tctttgtacg gcagagtaac aagacggcaa
     7801 cgatgttcac cgagtcggct tgcgatttcc cttgcggctt cacgaccaac atcatcaacg
     7861 tccatcgaga tgaatatttc ctcaaacctg tcgaggttat gatactcaaa ctcaatccac
     7921 tgttgcttag cgcctttccc gccaccaaac ggcacggata acgccgagat gccgtattgc
     7981 gcatagctca tacaatcaat ttcgccttcg caaagcacaa ccgccctcac gccagcgtcc
     8041 agagcctgcc atccgaacag gcaaggttcg caatcacctt
//
LOCUS       DAETPZ010000142         7875 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.143, whole genome shotgun sequence.
ACCESSION   DAETPZ010000142 DAETPZ010000000
VERSION     DAETPZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.143"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..648
                     /locus_tag="K0W59_003766"
                     /pseudo
     CDS             1..648
                     /locus_tag="K0W59_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            645..1031
                     /locus_tag="K0W59_003767"
     CDS             645..1031
                     /locus_tag="K0W59_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024614.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGDYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LACWFHYKDGQWRVITYIFWPPGARF"
     gene            1056..3268
                     /gene="tssI"
                     /locus_tag="K0W59_003768"
                     /pseudo
     CDS             1056..3268
                     /gene="tssI"
                     /locus_tag="K0W59_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
     gene            3291..3735
                     /locus_tag="K0W59_003769"
                     /pseudo
     CDS             3291..3735
                     /locus_tag="K0W59_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3758..4165
                     /locus_tag="K0W59_003770"
     CDS             3758..4165
                     /locus_tag="K0W59_003770"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HBI3024615.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4162..6640
                     /locus_tag="K0W59_003771"
                     /pseudo
     CDS             4162..6640
                     /locus_tag="K0W59_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            7019..7375
                     /locus_tag="K0W59_003772"
                     /pseudo
     CDS             7019..7375
                     /locus_tag="K0W59_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010380972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
     gene            7595..7798
                     /locus_tag="K0W59_003773"
     CDS             7595..7798
                     /locus_tag="K0W59_003773"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024616.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
BASE COUNT         2013 a         1821 c         2298 g         1743 t
ORIGIN      
        1 acaaatattg atggcaatat cattatacat aaggatggta ggaggacgta tttattaaat
       61 caaaatactg gttcgattac ggctgtgata agatggaagt atgagtagta taaggataat
      121 accgttacag atgactggac tatatgggaa aaagctattt ttatgcgagt gatatattta
      181 tctgttcagc aggcatggaa tgggaaaata acttattctg tttccggtga atctgagttc
      241 gctaaaaaat ttcagggtaa agctttgcct tttgatgtaa gaataatttc tgcatctcag
      301 aatgaagact ggttggttat tgcaacaaag gttttgcctg gtgctgattt aagaacatat
      361 gttgatttta aaaacagtac ggttcatgtt gattcagcgg acctggaaaa agtagcgaaa
      421 tgtattaact gtaataatac gttgcaagta aatatacctc atgaagccgg acatgtactt
      481 ggttacttag atgatgatta tgattcttcg tctccgtatg ttggagatat tagtgggttg
      541 atgaatgtgg gaatggagct tcgggaacga taccttaaaa acgcaactat aacgttgaat
      601 gtcataatgc ctgaaactaa atttacttta ttgaatgtaa ccaaatgaaa tttttctgta
      661 ttatagtgat tttcctttca ataaattaca tagctatagt gaatgttttc aagtcttctg
      721 cagttgctca atccaccaaa tattatgaag aaaatgatat acatgatgga agtaatgaca
      781 tcttttttat gaagaaatat gggccgcctg ctcatgagtt cgtgaggcca cgatataaat
      841 ttgttggtga ttattatatc ggcttgaatc aaacttatcg cataacagat cctgaatttc
      901 aagatgttcc tattaaggaa atgttctgga atttgcaggg ggatttgaat ctggcatgtt
      961 ggtttcatta taaagacggg caatggagag tgattactta tattttttgg ccaccagggg
     1021 ctagatttta atccggtatt aaaggagcaa atatcatgag ttttgtatct acgggtaata
     1081 aaccagttgg aaacggtgga cccgtcatca ccacaccacc aatagctggc gaaagtggcg
     1141 gtatgagcac aggttcagct gtaaccgatg tatctggtgc cgccgaggag atggcagaac
     1201 aggcagcggc ggacctgttt ggtgcgctgc cggaaccgtc aggtctggtt aaagccgcag
     1261 tagctgctgc gcaggctgcc gctgcagcag cagggatctc ggatatggct ggtgcagttc
     1321 aggatgcggc ggcgtcgctt gcagccggtg gtccgggggc gcataacgtc acagtaagtg
     1381 gtagtgcggt gccgccgcag atgcttttgt ttgcaggcat gaatggttcg gaagggcttg
     1441 ggaatctgtt ttcttacaca gtacagctta aaacgctgga cgccctgaac ctggggtacg
     1501 tttcccccgc tgctaacctg ccgcttcagc tcatggtggg caaagacctg tgcgtcagta
     1561 tcgaactgga cggcggcgga aaacggtata tcagcgggct ggtgacggcg gcgagggtgg
     1621 tcggccatga gggccgttcg gtaacgtatg aattacgcat tgagccatgg ctgaagctgc
     1681 tgacccatac cagcgactac aaagcgttcc agaacaaaac cgtggtggat attctcgatg
     1741 aagtactgga tgaatattcc tttccggtgg aaaagcggct ggtggaaaat tatccacccc
     1801 gcgcctggca ggtgcagtat ggtgaaaccg acttcgattt catccagcgc ctgatgcagg
     1861 aatgggggat ttactggtgg tttgagcaca gcgagaacag ccacacgcag gtgctggtgg
     1921 atgccatcaa tggtcataaa gcgtgccctg actcgccgct ggtggagtgg caccaggaag
     1981 ggctgaaact ggataaggaa tttatccaca ccattacggc cagtgagagg ctgcgtactg
     2041 gaaagtgggt gatggacgat tttgatttta tgaagcctcg ttcgctgctt aaaagtaccg
     2101 tggccagtcc gcgtgatacc ggtcatgctg aatacgagca ttacgaatgg cctggtgatt
     2161 acttcactac gggcgaaggc gagatgctga cgcgcattcg tatggaagcg cagcgcagcc
     2221 caggcagccg gcacatgggg ccggtcatat ccgtaccctc atgaccgggt acacctttac
     2281 gttgatgaat cacccgacgg cagaaattaa tcaggaatat ctgctggtac agaccactct
     2341 gtttttacgg gataatgcgc agcacagcgg tcaggatcag cattttacct atgtcaccac
     2401 gtttgaactg ccccccacca gcgaagttta ccgcccacaa cgaaccctga gcaaacccca
     2461 caccaaaggc ccgcagagcg ccatcgtcac cggcccggcg gggcaggaaa tctggacgga
     2521 taaatatggc cgggtgaagg tgcagtttgg ctgggaccgt tacggaaaga atgacgaaaa
     2581 cagttcctgc tggatccgcg tcagctatcc gtgggcgggt aaaggattcg gcatgatcca
     2641 gatcccgcgt attggccagg aagtactggt ggatttcaaa aacggcgatc cggatttgcc
     2701 catcatcgtg gggcgcacct ataaccagga caccatgccg ccgtgggggc tgccgggcat
     2761 ggcgtcgcag agtgggatat tcagccactc attgcaggga gggccgacta acggaaatat
     2821 gctgcgcttc gatgataaaa cgggtgcgga agaggtgaag ttccatgcgg aaaaagacct
     2881 caataccacg gtaaaaaaca atgaaaccca cacggtcatg gtcgatcgca taaaaaccat
     2941 tgtcaaaaat gaaaccatca gcgtgggaga agaccgtaat gccaccatca caaaaaatga
     3001 tggtctttcc gtcaaactgg ctcaaaccat caatgtcgga acgacatatc gtctggatgt
     3061 tggcgatcaa tttacccttc gttgcggcaa tgcagcgctt gtattacaca aggatggctc
     3121 gattgagttt tgcggaaaac aactgttact gcacaccagc gatgttatgc aactcattgg
     3181 cagtggcatt gatatgaatc cggatggcgg cacagccatg actgcggatg atattgcccc
     3241 gttacctacc cccgacgttt ctgaataaaa aatataaaag gaatttttct atgaccagcc
     3301 tttaccgtat tcaggagggc tgctttgccc tgcctgaaac ttttcttgat cgcaccgtca
     3361 atatcttcgt cccatcgggc aatgaaaggg caactcccag tctgaatatt tcccgcgata
     3421 cgctcaggcc agatgaaagt ctgaccacct atattgaccg ccagatagcc ttgatgaaaa
     3481 aaaaacctgg gccagcaccg ggtactttcg cggggaacgg cactggcggg aactggcgaa
     3541 gctgccatga caggtgaaca gattctcgca actcatcgtt ctggaaaaac agaagtgtac
     3601 cagcgtcagg ccgggtttat caccgggcca gcaaaggtac taatgttgac gctgaccact
     3661 cagcgtcctt ttgatgatca caccgaccag ctatggactg catggttgac tagttttcag
     3721 cctgctaaaa gctaaaaaaa aacagggagg ttttgtcatg tatgaagccg cacgggtgga
     3781 tgaccccatc taccacacca gcgcccttgc cgggtttttg ataggggcca tcataggtat
     3841 cgccattatt gccgtcgcca gctttgcttt ttttacctgc ggctttttgg ccgggctgat
     3901 tttgagtttt ctggctgatc agatagcttc cggagtcctg caactgggtg aggctattgg
     3961 gcgctcgata cattccacag tgggaaaaat tctgaccggt tctgataatg tcagcaccaa
     4021 cagtcgcccg gccgcccggg ccgtactgag tacggtgata tgcgaagacc attccccgga
     4081 aatccgcatt gcccagggtt ccggcaacat ctacatcaac agccagctgc ccgtaaggat
     4141 gaccacactg agtgtgatgc agtgattgag gacggctccc ccaatgtctt tctcggcggc
     4201 ggtacgcaga cggtactgga aatcagcccg gaaatccctg actggctgcg tcaggtggtg
     4261 gatgtgctgt atgtggtcgc cggaatgctt ggcgggctgg ctggcgcctg gcgtcaggcg
     4321 gcaaagatgg ggtcgaaatt tggtacaaaa tgtgcggcaa aatttattgg tggcgaaatg
     4381 gtcgggatgg ccatctctga cacagtcatg ggattgttca gcaatccggt agatgtcacc
     4441 actgggcaaa aaattttgct gccggagacg gattttaccc tgccaggacg cctgcctgtc
     4501 acctgttcgc gcttttacgc cagccacctg gaaactgaag gattgctggg cagaggatgg
     4561 cggctgaact gggagataaa cctgcgtgag gatgaaacat atatcacctt catcggtgta
     4621 cagggtcggg aactgagtta tccgaaagag atgctgatac cgggccacca gatattcgac
     4681 ccggaagagc agttttacct gagccgtctg catgacggat gctatgtgct gcattacacc
     4741 gaccgcagct attacgtgtt tgatgaattt gatgaccacg gcgtcgcgcc gttacttttt
     4801 atggagacgc cgtaccggca gcgtattgct ttcgggcgtg agaacggcag gctggtgcgg
     4861 gtggcctcca gcagcggaca ccatctgtta ttgcaccgca cgatgacgca agcaggggaa
     4921 cgcctgtcgc atattgaact tgtgaagggc gggcgtcccg gtaacctggt ggaatatcgt
     4981 tacgacgata acgggcaact gactggcgtg gtgaaccggg ctggtgtgac ggtgcgccag
     5041 tttgcctatg aaaatggcct gatgacagag catcgcaacg cgacgggctt tacctgtacc
     5101 taccactggg aagagattga aggtttcccg cgcgtggtgg aacacacgac cagcgacggc
     5161 gaggattacc ggttccacta tgattttgct ggcgggcaga cggtggtaac cggcaggccg
     5221 gagcagaagt ggcagtggtg gtttgatgaa gagacgtatg tgactgcgca ccggacgccg
     5281 ggcggcggaa tgtaccgctt cacgtacaac gaaaaccatt ttccggtagc ggtggaactc
     5341 cccggcgaac gcagggtaac gctggaatac gacaccctga gccgggtggt gaaagagacg
     5401 gacccggcag gccgggtgac acagacgcag tggaacggca gttttgctga aatcacgcgc
     5461 cgggcgctgg atgacgacca cgtatggaag gcggattaca acgaacacgg ccaggtgata
     5521 cgggagacgg acccggaagg gcgcgtcacg cggtacgggt atgatgaaca ggggctggcg
     5581 gtcagccgca cggatgcgcg gggcggcgag gttgtgctgg cgcacgatgc gcgcggtcaa
     5641 ctgaggcgtt acaccgactg ttcagggtgc gccacgggtt atgaatacga tgaagacggc
     5701 aacctgacgg cggtgacgga cgcggagggg aaaacggtac gcatcagcta taaccgtcag
     5761 gggctgccgg agacggtctg ccaccccggt aaacagcagg accgttacac ctggaacgcg
     5821 ctggggttac tgagcagcca ccgtcgcatc acgggcagtg tgcagtcctg gcagtatacg
     5881 ccgcgcggga tgctggcgcg ccatacggat gaagagaaac gggagacgcg ctggcagtac
     5941 accccggaag ggctggttgc cagtctgagc aacggcaacg gggctcagta ccggttcagc
     6001 tacgacggcg acggacggct gacgggagaa cagcgcccgg acggcctgat aaggatgttt
     6061 gcgctgaatg cggacgggtt tccggtcatc atccagacgc agggcacaga gggcggggtg
     6121 cgcaatgaac agcaggagcg tgatgcgctg ggccgcctgc tgcgcagtga cacgcagcac
     6181 agtacacgga ccttcagcta caaccgtctt gaccagataa cggaagtgac gctcaccccc
     6241 acggaagaag gggaacggct gcaccacatg caggcggaca cggtgcgttt tgcgtatgac
     6301 cgcagcgggt ggctgacggc agagcacagc gtgcacggca gtatcaaata ccggcgcgat
     6361 gcgctgggca atcccaccga tatcacgttg ccggacgggc agcatctcag tcatctttac
     6421 tacggcagcg ggcatctgct ccagacggca ctggacggta tcacggtcag tgaatatgag
     6481 cgtgacagcc tgcaccgtca ggtgatacgc acgcagggga aactggcgac gttcagcggc
     6541 tacaacgccg acaaccggtt aagctggcag cgcagcctgc caggtggcag ccagaatcca
     6601 cagcaggccg gtactccccc ggcgcagaac gacagcgtga cggcgcggga ctatatctgg
     6661 aacgcagacg gtgaagtcgg cggcatcaat gacaaactgc gcggttgcct ggtgttcagc
     6721 tatgaccgca gcggctggct gaccagccgc acggggcaga tgtatgacca tgaccattat
     6781 tattacgaca aagcgggcaa cctgctgacg gatgagtatc agggagcggt gatggataac
     6841 cgcctgccgg gctacgggcg tgaccgttac cgctataacg aatggggcga actgacagag
     6901 cgcagggacc agcaactgga gtggaacgcg cagggccagt taacgcgggt catcagcagc
     6961 aacagcgata cgctgtatca gtatgatgcg ctgggcaggc gcatcagcaa ggtgacatat
     7021 ggccgccatc acggggcgcg gagagagcca gaaaatctgg tattaccaca cggacctgac
     7081 gggcacggta cacgaggtaa ccgcgccgga cgggacgctg gtgtgggcgg gctaccaggc
     7141 gggatttggt gaaaaccggg gcgacatcag caacagcggg gcgtactttg agcagccgca
     7201 gcggctgccg gggcagtact ttgacgaaga aacagggctg cattacaatc tgttcaggta
     7261 ttatgcgccg gagtgcggga ggttcgtaag ccaggacccg ataggattaa acggtgggtt
     7321 gaacttatat gcttatgcgc ctaatccgtt aggttggatc gatccgctag gactgaattc
     7381 tttaggaata gattcaaata atatatttag aggagatggt aactataaag gtggcggaat
     7441 tggacagcca aagagtggca caatatcagc acaggacatt attgatcatc tctaagaaaa
     7501 aggtgatggg cgtttaacat cttttagtac taaaaaaaca tctagttcca actctcgtgg
     7561 tgcagatttt ttctcaaaaa accatgtctt caaagtggca gtttctgatc tcgatgattt
     7621 agttaaaaat aatcagatca ctgttttaac acaagaaaaa gtaaaagcaa tatttggttt
     7681 agaatcaagc aagaaaatta gaaaagaact tggtaatatt cttcagaata tggctaggaa
     7741 taatgaggtg ttagttcatg ggcaaattct tggaaataaa gttagccgat gtaattagtt
     7801 agtggagagg aagttgtgga cgaaattaga ataagaattg ctggtaatac ggctgcgcct
     7861 tgtttccatg tgcta
//
LOCUS       DAETPZ010000143         7821 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.144, whole genome shotgun sequence.
ACCESSION   DAETPZ010000143 DAETPZ010000000
VERSION     DAETPZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7821
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.144"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            211..429
                     /locus_tag="K0W59_003774"
     CDS             211..429
                     /locus_tag="K0W59_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBI3024617.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            647..889
                     /locus_tag="K0W59_003775"
     CDS             647..889
                     /locus_tag="K0W59_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024618.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVLGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1219..2148)
                     /gene="fdhE"
                     /locus_tag="K0W59_003776"
     CDS             complement(1219..2148)
                     /gene="fdhE"
                     /locus_tag="K0W59_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HBI3024619.1"
                     /translation="MSIRIIPQDELGSSEKRTANMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2145..2780)
                     /gene="fdoI"
                     /locus_tag="K0W59_003777"
     CDS             complement(2145..2780)
                     /gene="fdoI"
                     /locus_tag="K0W59_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBI3024620.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2777..3679)
                     /gene="fdoH"
                     /locus_tag="K0W59_003778"
     CDS             complement(2777..3679)
                     /gene="fdoH"
                     /locus_tag="K0W59_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HBI3024621.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3692..6742)
                     /gene="fdnG"
                     /locus_tag="K0W59_003779"
     CDS             complement(3692..6742)
                     /gene="fdnG"
                     /locus_tag="K0W59_003779"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6155..6157),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBI3024622.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6936..7769
                     /gene="fdhD"
                     /locus_tag="K0W59_003780"
     CDS             6936..7769
                     /gene="fdhD"
                     /locus_tag="K0W59_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBI3024623.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1814 a         2013 c         2077 g         1917 t
ORIGIN      
        1 aatataatat attcatagca cccataactc atcctgggcg ataactaaac catttttgag
       61 taataaatga aacaactgca acgctcttat tatttctgga aaacgcctcg caaaaacaaa
      121 atgacatgca aaaatccgca tttttttaaa tgctttttcg gcgtaatttt cgcacataca
      181 ttaatgagta atatatgtaa ggtggtggta atggcgatga atacggtttt tcttcattta
      241 tcagaagagg caattaaacg gctgaacaag cttcggggct ggcgtaaggt ttcacgatct
      301 gcaattttac gcgaagcggt tgagcaatat ctggagcggc agcaatttcc ggtgcgtaaa
      361 gcaaagggag gcaggcaaaa gggggaggta gttggtgttg atgatcagtg taaggagcat
      421 aaggaatgaa aaaaattttt cattcttgaa tataaaaaac agatgccctt attctggtat
      481 taatacaagg ctgttttact tgaacttata ataactgcaa ctgttacatc gtatctggaa
      541 aacgcctcgc aaaaataaaa aatgatgcgt aaatgagctt tctttaaaga ttcggcaagg
      601 gtacttttct ctccatagat tcaaaaatga tattggcgag atatttatga actctctggc
      661 aggtatagat atgggcagaa ttttactcga tttatcgaat gaggtgatta agcaacttga
      721 tgatctcgag gtgcagcgta atcttcctcg tgcggaccta ttaagggaag cggtagatca
      781 atacctgata aatcaatcgc aaacagcaag aaccagtgtt cttggcatct ggcaagggtg
      841 tgaggaagat ggtgtcgaat atcagcgtaa gctgcgcgag gaatggtaat gggaaaaaat
      901 gatgtcaatc agattgctga caacgtgcgc gttgttcatg ccggatgcgg cgtgaacgcc
      961 ttatccggcc tacaaagtca tgcaaattca atatattgaa gtttattggt aggcctgata
     1021 agcgcagcgc atcaggcaat catacgcttg tcatcagtga catacccata accttatcga
     1081 tgtgttggag ggaagaacga ggcaaacaga caattgcggc atgatcttga atgggtaacg
     1141 cggaatacga gagatggtgt cgtcacaccc tatgagctgt aatccgggcg acctgcgcca
     1201 cccggaaaac gtcagagatt actctccttc acccggaaac aggaacgggt taatggaact
     1261 acgggcgtag ccttcttgct ccattcgcgc gtccagtacc agagaggcga ggtcatctgc
     1321 cacggcttca actttcggtt ctttttcctg ataaagaatc ttcaggtaag tgccgcagtc
     1381 atcgcagctt tcggctttaa tcgcggcctg ttcgtcatcc agcgaccagt aatgcagttt
     1441 gccgctctgc tcgcagttgc tgcattttac gcgcactacg tgccattcgg tttcacacag
     1501 gttgcagtgc aggtaacgca gaccctgagt ggtgccaatt tgcaccatgc tggacaccgg
     1561 catagagcca cataccgggc aatattgacg ttgttcgccg tattcagcgc gggctttgcc
     1621 ggggatcaga ttggccatct gcgcccagta gagcgacagc gcagcccaga taaacggcgc
     1681 tttatcgctg ctgacggacg agaaatcaga ggcaaacagc gcgctggcca tatcttccag
     1741 ctcctgagtc gatgccttct ccagattctc aatcactgcc agcgccgggc cgctcatttc
     1801 aggtttcagc tcagcaatta gcgccatcag cagcttttgc cagtgcttat cacgcggcag
     1861 aacgtgaata tccagcgggg gcttgccctg tgcgctggct tctttaatgc gcgcggtcag
     1921 atccatctcc agcggatggt cgtacagcac cacttcctgg gcgtgggcga taagcgcagc
     1981 aaagcgcagg taatcaccca gcggattatt ttctgccagc tcgcgcagac gctcggcgcg
     2041 gcggttgtat aaattcttga gccgagggaa caataacggc ggaatcatat tcgccgtacg
     2101 tttctcgctc gaacccagct catcttgcgg gattatgcga atactcattc agctttcttt
     2161 tccgttgtct tgcggacctc acggtaccag cgcgggtgat gtttcttcgc ccaggcgctg
     2221 gtaacccatc cttccaccat ggcggtaatc gtgcctttca cccaaagggc ggcgtagata
     2281 tgcaccatga taaccacaat taacgccact gcggcaaatg aatgcagcat taacgcgaat
     2341 cggatcaccg ggattgagaa agcaggcgca aaataaggac gccagataat cacgccgctc
     2401 accagcagca gaaccaggaa aataatcgcc gcccagaaaa cgcatttctg accgaagtta
     2461 taacgcccgg tgtcacctac ttcctcgttg acgacgatct tacgaatatt cttcgcccaa
     2521 aagatatcat cccgattgat taggttttga tgccagtaac ggaaaaacat gatgatgaac
     2581 gaggcaaaca taaccacgcc gacaaacggg tgcagaattc gcgccagctg cggtgtgccc
     2641 atgatttgca tcaaccagtt gaaggacggg aacaaaaagc ccagcccgct caccgccgcc
     2701 aggatgaagc agaaggcggt gatccagtgg ttgatacgtt ccggcgcggt gtagcgcacg
     2761 atggtgtcac gtcgtttcat ttgcgctcct cgtctttctc ttcgtgcaga ttattctctt
     2821 cctcatccgc acggttcgga ccgacaccca cgtagtggaa gatactggcc gcaaaggtag
     2881 ccgcaaagcc aacagccgcg agcggtttcc agatgccttt ccagaatttc acggtttcgc
     2941 tgatttccgg gttctccggc aagccatgat acagatttgg cttgtcagca tggtgcagca
     3001 cgtacatgac gtgtgtacca ccgacgcctg ccggatcgta cagacccgca ttgtcgtaac
     3061 cgcgggtttt cagctcagca acgcgctcgc tcgccagcgt tttcatcgac tctttcgtac
     3121 cgaagtgaat cgcgcccgtt gggcaggtct tcacgcaggc tggttcttgc ccaaccacca
     3181 cgcggtcaac gcacagcgta catttgtaga cgcggttgtc ttccgggttg aggcgcggaa
     3241 tgtcgaacgg acagcccgca atgcaataac cgcagccaat gcactgctcg gactggaagt
     3301 cgacgatacc gttggcatac tgaataattg caccttccgc cgggcacgct ttcaggcagc
     3361 ctggatcgga acagtgcata cagccgtctt tgcggatcag ccattccagt ttgtcgttct
     3421 gctccacttc cgagaagcgc attaccgtcc acgatttggc gcttaaatca ttggggttgt
     3481 cgtacacccc aatgttattg ccgacggtat cgcgaatgtc gttccactct gaacacgcca
     3541 cctgacaggc tttacagccg atacaggtgg taacgtcgat gagtttcgcc acttcttcct
     3601 ggaagtcccg cgcctgaggc gcgggggtca gaccgttagt cgcggaacga cgaatgatat
     3661 cttgagattg ataagccata tgtcgtctcc gttacacctt ttccacattc acaaggaagg
     3721 acttaaactc cggcgtctgc gtgttcgcat caccaacgaa tggcgttaaa gtattggcaa
     3781 taaagccttt tttagctacg ccttcatagc cccagtgaat cggaataccg atggtatcga
     3841 tatctttgcc gttcgctttc agcgtgcgaa tacgtttggt caccaccgct ttggctttga
     3901 tatagccacg gttggaggag actttcacgg tatcgccctg ggcaatgcca agtttattcg
     3961 ccagcgactc cccgatttcc acaaactgct ctggctgcaa aatcgcgttc aacagcgcgt
     4021 gtttggtcca gtagtggaag tgctcggtca gacgataggt ggttccgaca tacgggaact
     4081 tatcggcttt acccaatgct tcggcgtcgt ctttaaagat acgtgcagcc gggttcgaga
     4141 taacgtttgg atgcagcggg ttagttccca gcggcgtttc aaacggctcg tagtgttccg
     4201 ggaacggacc ttccgccatc ttatcgaggg caaacagacg ccccatgcct tcctgctgca
     4261 tgataaacgg cccgacgccg ctacccggag gcgctgcgct gtagtccgga atatcccagc
     4321 cggtccactt agtgccgtcc catttcagca actgacgctt cggatcccac gggttaccct
     4381 gcggatctgc ggaggcgcgg ttatacagaa tgcggcggtt aagcggccat gcccatgccc
     4441 agcccagcgt gttaccgagg ccagacggat cggcgttatc acgacgcgcc atctggttgc
     4501 cttccggcgt ccagctaccg gcgaaaatcc agcagccaca ggaggttgta ccgtcatcgc
     4561 gcaattgggc gaacgagcta agttgttggc ctttcttgac gataaccgca ccggttgccg
     4621 gatcggtaat atcggccagc gccttaccgt tgctctccat cgctacttct tccgacttcg
     4681 gctcatgcgg aatggcgtag ttccaggtca tgttcagcac ctgatccggg ttcgcgccac
     4741 cctgttcggc atacatcttg cgcaagcgca ggaagatacc ggagaggatc tcaccgtcag
     4801 tcagcgcaat ccccggggcg tccgcacctt tccagtgcca ctgcaaccag cgacctgagt
     4861 taacgattga accgttctct tctgcgaagc aggtcgatgg cagacggaac acttcggtct
     4921 ggatcttcga cgagtcaact tcgttcagct caccgtggtt ctgccagaag ttagaggttt
     4981 cagtgttcag tgggtcgatg gtgacgagga acttcagttt cgacagacaa ccgatcactt
     5041 tgtttttgtt cgggaatgag gcaacagggt taaagccctg gcagatatag ccattgacct
     5101 tgccctcttt catcatctcg aagtattgca ggacgtcgta gcctttatcc cacttcggca
     5161 accagtcaaa gccccagcta ttttccgccg tcgctttatc accaaagaag gccttcatca
     5221 tagagacgaa gaatttcggg tagttgcccc agtagtttac ctggccttcc agcagcggtt
     5281 ttggcgtgtt ggcggtaagg taggtttgca gatcggtctg cttctcgctt ggcagcgtca
     5341 tgtaacctgg cagactctgc gacagtagcc ccaggtccgt cagcccctga atattggagt
     5401 gaccgcgcag ggcgttaacg ccgccgccgg ccatccccat attgccgagc agcagctgga
     5461 tcatcgccat cgtacgaatg ttttgcgcac caacggagtg ttgcgtccag ccgagggcat
     5521 acaggaacga ggcagtttta tcgtgagcac tggtttctgc gatgtattcg cagactttca
     5581 ggaacgcgtc ttttggcgta ccacagatgt tttcaaccac atctggcgtg tagcgggaaa
     5641 cgtgctgttt cagcaagttc cacacgcagc gcggatgttg cagcgtggta tcgcgtttgg
     5701 cgaagccgtt ttcgtccagt tcataagtcc aggaggattt atcgtacttg cgtttttccg
     5761 cgtcgtagcc ggtgaacagg ccatcttcaa agccgtaatc ctcacgcacg atcaggctgg
     5821 cgttggtata ggcttcggtg tattcgtggt tgaatttttc attgttcagc aggtacagca
     5881 atacgcctga caggaaagca atgtcagtac cggaacgaat aggggcatag tagtcagcta
     5941 ccgccgccgt acgcgtaaag cgaggatcga tcacaatcag cttcgcgccg ttgtgaattt
     6001 tggcttccat cgcccagcgg aacccgaccg ggtgagcttc agcggcgtta ccgcccatca
     6061 ccacgacgag gttggcgttc ttgatgtcga cccagtggtt ggtcatcgca ccgcgaccaa
     6121 atgttggagc aagacttgct accgttggtc cgtgtcagac acgcgcctgg ttgtcgaccg
     6181 cgagcatacc cagcgcgcgg gagaattttt gcgttaaata gccggtttcg ttactcgacg
     6241 cggaagcaca cagcatcccg gtggagagcc agcggttaac agtcacgcct tcggcgtttt
     6301 gcgcaatgta gttagcatcg cggtcttctt tcatcagttt ggcgatgcga tcaaacgcct
     6361 cttcccaact gatttgctgc catttatcag aacctggcgc acggtattcc ggaaacttca
     6421 gacggctttc ggagtggatg aaatccacca ggccagcgcc tttcggacaa agtgcaccgc
     6481 ggttgaccgg gtgatccgga tcgccttcga tatggaagat agatgctttg gcgttttttg
     6541 ctccgtcacc gaggctgtac atcaacagcc cacagcctac ggaacaatag gtgcaggtat
     6601 tacgggtttc gcgggtgcgc agcagtttat actgccgggt ttccgcgagt gctacgctgg
     6661 gtgcaaaacc cagtgccgct gccgtggtgc ctgccatacc gccagcgcag atcttaaaga
     6721 actgccttct gctgacctgc atggattgct ccttgtttcg acattgtcac gtcccattta
     6781 cattcgcttg ctgcgtgtgc agggagtggg agttattttt ctttgcggaa ggggccgcaa
     6841 aggtccagaa ttggctcaat ttccctccat ctaggaaggg tttgtaacag aataccataa
     6901 tgttggtgtg tgtgttctta tctggttaag agaaagtgaa aaaaacacag cgaaaagaaa
     6961 tcgaaaatgt gacaaatatc acaggtgttc gtcaaattga gttatggcgg cgtgatgatt
     7021 tacaacaccc acggttagat gaggtcgcgg aagaagttcc cgttgcgctg gtctacaacg
     7081 gcatttcgca tgtggtgatg atggcgtcgc ccaaagacct tgagtacttt gcgctcggtt
     7141 tttcgctttc cgaggggata atagaaagtc cgcgcgatat cttcggcatg gatgtcgttc
     7201 cttcctgtaa tggtcttgaa gtacaaattg agctttccag ccgccgcttt atggggttga
     7261 aggagcgccg ccgggcgctg gcgggacgta cgggctgtgg cgtatgcggc gtggagcaac
     7321 ttaatgacat cggaaaaccg gtgcagccgc taccgttcac ccagacgttt gatctcaaca
     7381 aactggatga tgcattacgt catctcaacg attttcagcc agtggggcaa ctgactggtt
     7441 gtactcacgc cgctgcctgg atgttgccat ctggcgaact ggtcggcggg catgaagacg
     7501 tgggtcgcca tgtggcgctg gacaaactgt taggccgccg gtcacaagaa ggggaaagct
     7561 ggcagcaagg tgcggtactg gtttccagtc gtgccagtta tgaaatggtg caaaagtcgg
     7621 cgatgtgcgg cgtagagatt ttgtttgcgg tgtctgccgc gaccacgctt gctgtagaag
     7681 tggctgagcg ctgtaatctg acactggtag gtttttgtaa accgggtcgg gcaacggttt
     7741 atacccatcc gcagcgtttg agcaattaat taaaataagt cctgcgatat atattaaatt
     7801 gcaggactta ttcatttcgt g
//
LOCUS       DAETPZ010000144         7564 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.146, whole genome shotgun sequence.
ACCESSION   DAETPZ010000144 DAETPZ010000000
VERSION     DAETPZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.146"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..1678
                     /gene="cpdB"
                     /locus_tag="K0W59_003781"
     CDS             <1..1678
                     /gene="cpdB"
                     /locus_tag="K0W59_003781"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HBI3024624.1"
                     /translation="KGLKAGDVHPVYKALNTLDYTVGTLGNHEFNYGLDYLKNALAGA
                     KFPYVNANVIDARTKQPMFTPYLIKDTEVVDKDGKKQTLKIGYIGVVPPQIMGWDKAN
                     LSGKVTMNDITETVRKYVPEMREKGADVIVVLAHSGLSADPYKVMAENSVYYLSEIPG
                     VNAIMFGHAHAVFPGKDFADIEGADITKGTLNGVPAVMPGMWGDHLGVVDLQLSNDSG
                     KWQVTQAKAEARPIYDIANKKSLAAEDSKLVETLKADHDATRQFVSKPIGKSADNMYS
                     YLALVQDDPTVQVVNNAQKAYVEHYIQGDPDLAKLPVLSAAAPFKVGGRKNDPASYVE
                     VEKGQLTFRNAADLYLYPNTLIVVKASGKEVKEWLECSAGQFNQIAPNSTKPQSLINW
                     DGFRTYNFDVIDGVNYQIDVTQPARYDGECQMINVNAERIKNLTFNGKPIDPNAMFLV
                     ATNNYRAYGGKFAGTGDSHIAFASPDENRSVLAAWIADESKRAGEIHPAADNNWRLAP
                     IAADKKLDIRFETSPSDKAAAFIKEKGQYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(1804..2184)
                     /locus_tag="K0W59_003782"
     CDS             complement(1804..2184)
                     /locus_tag="K0W59_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBI3024625.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            2273..3133
                     /gene="qorB"
                     /locus_tag="K0W59_003783"
     CDS             2273..3133
                     /gene="qorB"
                     /locus_tag="K0W59_003783"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBI3024626.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAALVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIARPTTTLAE
                     SVSHLFNVNN"
     gene            3242..4207
                     /locus_tag="K0W59_003784"
     CDS             3242..4207
                     /locus_tag="K0W59_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBI3024627.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLSQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            4315..4977
                     /gene="ytfE"
                     /locus_tag="K0W59_003785"
     CDS             4315..4977
                     /gene="ytfE"
                     /locus_tag="K0W59_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HBI3024628.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(5022..6434)
                     /gene="cycA"
                     /locus_tag="K0W59_003786"
                     /gene_synonym="dagA"
     CDS             complement(5022..6434)
                     /gene="cycA"
                     /locus_tag="K0W59_003786"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HBI3024629.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDFSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(6742..7362)
                     /gene="fklB"
                     /locus_tag="K0W59_003787"
     CDS             complement(6742..7362)
                     /gene="fklB"
                     /locus_tag="K0W59_003787"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBI3024630.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
BASE COUNT         1944 a         1975 c         1929 g         1716 t
ORIGIN      
        1 gaaaggatta aaagcaggtg atgttcatcc ggtttataag gcgctgaata cgctggatta
       61 tacggtcggt acactcggca atcatgaatt taactacggt ctggattacc tgaaaaatgc
      121 gctggcggga gcgaaattcc cttatgtaaa tgccaacgtc attgacgcca gaaccaaaca
      181 gccaatgttt acaccgtatt taattaaaga taccgaagtg gtcgataaag acggaaaaaa
      241 acagacgctg aagattggct atattggcgt cgtgccgccg caaatcatgg gctgggataa
      301 agctaattta tccggaaaag tgacgatgaa tgatattacc gaaaccgtgc gcaaatacgt
      361 gcctgaaatg cgcgagaaag gtgccgatgt cattgtcgtt ctggcgcatt ccgggctgtc
      421 tgccgatccg tataaagtaa tggcggaaaa ctcagtttat tacctcagtg aaattccggg
      481 cgttaacgcc attatgtttg gccatgctca cgccgttttc ccgggtaaag attttgctga
      541 tatcgaaggg gctgatatca ccaaaggcac gctgaatggt gttccggcgg taatgccggg
      601 catgtggggc gatcatcttg gggtggtcga cttacaactc agtaatgaca gcggtaaatg
      661 gcaggtgacg caggcgaaag cggaagcacg gccgatttac gacatcgcca ataaaaaatc
      721 cctcgcggcg gaagacagca agctggtaga aacactcaaa gccgatcacg atgccacacg
      781 ccagttcgtc agcaagccaa tcggtaaatc tgccgacaat atgtatagct atctggcgct
      841 ggtgcaggac gatccgaccg tgcaagtcgt gaacaacgcg caaaaagcgt atgtcgaaca
      901 ttacattcag ggcgatccgg atctggcaaa actgccggtg ctttcagctg ccgcaccgtt
      961 taaagttggt ggtcgcaaaa atgacccggc aagctatgtg gaggtggaaa aaggccagtt
     1021 gaccttccgt aatgccgccg atctttatct ctaccccaat acgctgattg tagtgaaagc
     1081 cagcggtaaa gaggtgaaag agtggctgga gtgctccgcc ggacagttta accagattgc
     1141 tcctaacagc acgaaaccac agtcactcat caactgggat ggtttccgca cttataactt
     1201 tgatgttatt gatggtgtga attatcagat tgatgttacc caacccgccc gctatgacgg
     1261 cgagtgccag atgattaatg tcaatgcaga gcggattaag aacctgacct ttaatggcaa
     1321 gccgattgat ccgaacgcta tgttcctggt tgccaccaat aactatcgcg cttacggcgg
     1381 caaatttgcc ggtacgggcg acagccatat cgcttttgct tcaccggatg agaaccgctc
     1441 ggtgctggca gcgtggattg ctgatgagtc gaaacgtgcg ggggaaattc atccggcggc
     1501 agataacaac tggcgtttag caccgatagc tgccgataag aaactggata tccgtttcga
     1561 aacttccccg tcagataaag ccgcagcgtt tattaaagag aaagggcagt atccgatgaa
     1621 taaagtcgcg accgatgata tcgggtttgc gatttatcag gtggatttga gtaagtaaaa
     1681 cacttctttt tcggcctata aatcaaccgc atccggcatt tattggcgga tgcgatgctg
     1741 ccgtatctta tccaccctac aagccatgaa ccgtagaccg gataagcgca gcgcatccgg
     1801 cagttatgcc gcacgttcat cccgcaccgc caacacctcg ggcaaattca actcaatcca
     1861 gtccgccagt gcagcaacct tttcgctcac ctgctcgccc agcggcgtga ggctatattc
     1921 cacatgcggc ggcaccaccg gatacgcgat acggttaaga aaaccatcct gttctaacgc
     1981 ctgtaacgac tgcgcaagca tcttttcact caccccaccg attttgcgcc gcaggtcgct
     2041 aaagcgatga gtaccttcgc gtagcgccac cagaatcaac accccccaac ggctggtgac
     2101 gtgtttcaac acctcgcgcg acgggcactg ttccgcaaag agattaccct ctttcagttg
     2161 ttgtgacagg ctaacctgac tcatttcata cttacctttt tgcatgtact tactaaaagt
     2221 aagtttaggt gttagcgtat ttaaacacaa gacaaaacga tggagacttc ccatgatcgc
     2281 tattactggt gccactggcc aacttggtca ctatattatt gaatccttga tgaaaacggt
     2341 tcctgccagc caaatagtgg ctatcgttcg taatccggca aaagcccagg ccctggcagc
     2401 acaaggcatt accgtgcgtc aggctgacta cagcgatgaa gccgcactga catctgcact
     2461 tcagggagtg gaaaaactac tgctgatctc ttccagcgaa gtgggtcaac gtgccccgca
     2521 gcatcgtaat gttattaatg ccgcaaaggc ggctggcgtg aaatttatcg cttataccag
     2581 cctgctacat gcagatacct ccccgctcgg cctcgccgat gagcacatcg agacggagaa
     2641 aatgctggct gattctggca tcgtttacac cctgctgcgc aacggctggt acagcgaaaa
     2701 ctacctcgcc agcgccccgg cagcactgga acacggcgta tttatcggtg cggcgggcga
     2761 tggcaaaatc gcctcagcaa cgcgggcaga ttatgcgaca gctgcggcac tcgtgattag
     2821 cgaagccggt cacgaaggca aggtttacga actggcgggc gatagtgcct ggacgttgac
     2881 acagttagcg gcagagctga ccaaacagag cggcaaacag gttacctatc aaaatctgag
     2941 cgaagccgat ttcgccgcgg cactgaaaag cgtcggactg cccgacggac tggcggatat
     3001 gctggcggat tctgacgttg gcgcatcgaa aggtggtctg tttgataaca gcaaaacgct
     3061 tagcaaattg attgcccgcc caacgacaac gttagccgaa agcgtaagcc atctttttaa
     3121 tgttaataac tagttaatta aagtggcatc ctcccgcatc ctctctgata atgacgggat
     3181 gccgggagca atcatgtctg cttcctgaac gtttcttctg acagaccaat ggatgccagt
     3241 aatgattagc ggcgtgctgt acgccctgtt agcagggttg atgtgggggc ttatttttgt
     3301 cgggccgttg atcgtgccgg aatacccggc gatgttacag tcgatggggc gttatctggc
     3361 gttagggtta attgcgctac ccattgcctg gctgggacgc gtgcgtctgc gtcagttggc
     3421 gcgtcgggac tggcttaccg ccttgatgct cactatgatg ggcaacctca tttattactt
     3481 ctgccttgcc agtgccattc aacgtactgg cgcgcctgtt tccacgatga ttatcggcac
     3541 cctgccggtg gtgattcctg tctttgccaa tctgctttat agccagcgcg acggcaaact
     3601 cgcgtgggga aaactcgccc cggcactgat ttgtattggc atcggcctgg cgagtgtgaa
     3661 tattgctgag ttaaaccacg gactccccga ttttgactgg gcacgttata cctctggcat
     3721 cgtgctagcg ttagtttccg tggtctgctg ggcatggtat gccctgcgca acgcccgctg
     3781 gctgcgggaa aatcccgaca aacatccgat gatgtgggcg acggcgcagg cgctggtcac
     3841 gctgccggtt tctctcatcg gctatctcgt cgcctgttac tggctgaata tacaaacgcc
     3901 ggacttctcc ttaccttttg gcccccgtcc gctggtgttt attagtctga tggttgcgat
     3961 agccgtgctt tgctcatggg ttggcgcact ctgctggaac gtcgccagcc agcgattacc
     4021 gacagtgatt ctcgggccgc tgattgtttt cgaaacgctg gcaggtttgc tgtacacctt
     4081 tttactcagc cagcaaatgc cgccgctaat gacgctgagc ggtatcgcgc tgttagtgat
     4141 tggcgtggtc atcgcggtca gagcaaaacc agaaaaacct ttaactgaat ctgtctcaga
     4201 aagttgacat gctggcagtg agttaaataa gcctctgcta cgtaagggtt atagcttttg
     4261 ccttaaaggt gtatttaaaa tacatcttat cttattaaga atgaggtatc agctatggct
     4321 tatcgcgacc aacctttagg tgaactggcg ctctctatcc ctcgcgcttc ggctctgttt
     4381 cgtaaatatg atatggatta ctgctgtggc ggtaagcaga cgctggcgcg cgcggcggca
     4441 cgtaaagaac tggatgttga ggtcattgaa gctgaactgg caaagcttgc tgaacaaccg
     4501 attgagaaag actggcgtag cgccccgctg gcagaaatca ttgaccatat catcgtgcgc
     4561 taccacgatc gtcaccgcga gcagctgccg gagctgatcc tgcaagcgac taaagtcgag
     4621 cgcgttcacg ccgacaaacc gagcgtgcca aaagggctga caaaatacct gaccatgctg
     4681 catgaagagc tttccagcca catgatgaaa gaagagcaga tcctcttccc gatgatcaaa
     4741 caaggcatgg gcagccaggc aatggggcca atcagcgtaa tggaaagcga gcacgatgaa
     4801 gcgggcgaac tgctggaagt gatcaaacac actaccaata acgtcacacc gccgccagaa
     4861 gcctgcacca cctggaaagc gatgtataac ggcattaatg aactgattga tgacctgatg
     4921 gatcacatca gtctggaaaa caatgtactg ttcccacgcg ccctagcggg tgagtgatct
     4981 aaaagctgga tggcattgcg ccatccagca tgataatgcg gttatttccg cagttcagca
     5041 gcccgcttct taccaataaa cagccagccc aaccccagcg cgataaacca cagcggggta
     5101 accagcagcg cctggcgagt gtcatcttcc agtgtcagca acacgaccac gaacacaaag
     5161 aacgccatac atacccagca catcagcttg ccgagcggca tcttgtagat cgacttttca
     5221 tgcagatgag gacgctgttt gcggtacacc aggtacgagc aaaggataat cgtccagacg
     5281 aacataaaca gaatcgcgga aacggttgta atcatcgtga acgcgccaat cacgctagga
     5341 ttcacataca acatcaccac gccgccgagc agacagatac acgagaaggt caaccctttc
     5401 gcgggtactg cgcgcttaga aagtttagcg aacgctttcg gcgcaacacc ttcctgcgcc
     5461 agaccaaaca gcatacggct ggtagagaag acaccgctgt tagcggaaga cgccgcagag
     5521 gtcagcacca caaagttgat cacgctggca gccgcaggca aacctaccaa cacaaacagc
     5581 tcaacgaacg ggcttttctc cgggactacc gaactccacg gtgtcacgga cataatcaca
     5641 atcagcgcga agacgtagaa cataatgata cggatcggaa tggagttaat cgcgcgtggc
     5701 agtgatttct ccggatcttt ggtttccgca gctgttgtac ctaccagctc aatccccacg
     5761 aaagcgaaaa ccgctatctg gaatccggca aagaagccac ttaaaccttt cgggaaccag
     5821 ccgccgtcat tccacaaatg cgcgaatgac gcttccacac cggtcggtga ctgaaagtgc
     5881 atcgccacca tgaccaggcc gacgacaatc agcgacacga tggcgacgat tttgatcatc
     5941 gcaaaccaga actccatctc accgaacatt ttcacggtgg caaggttgag cgtcagcagc
     6001 agcactatca ccgccagcga ggcgacccag tcggagaaat cggggaacca gaactgagca
     6061 taagccgtga tcgccaccac gtctgccata ccggttacaa cccagcagaa ccagtaagtc
     6121 cagccggtga aatatcccgc ccacggcccc agtaaatcgg aagcgaagtc actaaaagat
     6181 ttgtattcca gattcgaaag cagcaattcc cccattgccc gcatcacgaa aaagagcata
     6241 aaaccaatga tcatataaac gaaaatgatc gacggcccgg caaggctaat cgttttgccg
     6301 gaccccataa acaacccagt accaatggca ccgccaatgg caataagctg aatatgtcgg
     6361 tttgtgagat tgcgccgtag cgactgttca gccggagcct gatcatcggc aacgactttt
     6421 acctgatcta ccatgttttt tcttcctgta cctgtctgtg ttgttcaggc tctacggcct
     6481 ttagtcagtc tatgatacga cgaatctgta tcgggactca tcgatattag gtaacaatac
     6541 gcgggatgaa tactaagatt tagatctaat gttaatttta tgtttaaagt gagtgtcata
     6601 tcacggtgat aacgcgaaac agctcacaaa aatacacgct tacggggtct ttgcaacaca
     6661 aaatagcgcc gtttggcaaa ctgaagggtt tattgctgaa tgcctgctcc cctctggttc
     6721 gaggggaaca gaatgcgctg cttagaggat ttccagcagt tcgacttcaa acaccagggt
     6781 gctgaacgga gggatggatg cacctgcgcc gcgctcgcca tatgccagtt cctgcgggat
     6841 agtcagttcc catttagaac ctaccggcat cagagtcagt gcttcaatcc agccagggat
     6901 cacgccatta accgggaatt cagcgggttc accacgagca acggagctgt caaacacggt
     6961 gccgtcgatc agtttaccgg tgtaatgaac acgaacgcgg tcggtacgtg ccggaattgc
     7021 gccttcaccc tggttgatca cgcggaattg caggccagat tcggtgctat tcacaccttc
     7081 ttttttggcg ttttcttcca ggtatttcac accttcagca gccatcgcct ggaaacgctg
     7141 acgacgaacg gcatcggcgc gttcgtggat ttcacgcagc gcgcgatgca ccacatcaac
     7201 cggcacagcc ggatgtttgc cttccagcgc atcggcaata cctgcaacca gtgcttctgg
     7261 cagcagccct tccaggccag attcactcag ttgttgccct acctgcaaac caatgccgta
     7321 gcttgcttgc gcttcgatgg tgtcaaaagt tggggtggtc atcgtatttc ctttcttagg
     7381 gtataaaagt agcgggcagc atatcagcca cacttctttg ggtaaaactt tgtcgcagga
     7441 atgatgacaa atcccgggga aaaagaaaca atagcaatat cccgtatgaa tataataaag
     7501 gcgggttggt aatgctgcca acttactgat ttagtgtatg atggtgattt taaggtgctt
     7561 gcgt
//
LOCUS       DAETPZ010000145         7462 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.147, whole genome shotgun sequence.
ACCESSION   DAETPZ010000145 DAETPZ010000000
VERSION     DAETPZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.147"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            215..291
                     /locus_tag="K0W59_003788"
     tRNA            215..291
                     /locus_tag="K0W59_003788"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:249..251,aa:Arg,seq:tct)
     gene            complement(337..1098)
                     /gene="envY"
                     /locus_tag="K0W59_003789"
     CDS             complement(337..1098)
                     /gene="envY"
                     /locus_tag="K0W59_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HBI3024631.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(1281..2171)
                     /locus_tag="K0W59_003790"
     CDS             complement(1281..2171)
                     /locus_tag="K0W59_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HBI3024632.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            complement(2172..5144)
                     /gene="nfrA"
                     /locus_tag="K0W59_003791"
     CDS             complement(2172..5144)
                     /gene="nfrA"
                     /locus_tag="K0W59_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HBI3024633.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKAGDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTGAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPTNLPRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRELGNNAQYWWLHAQRYIPAQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKDPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5131..7373)
                     /gene="nrfB"
                     /locus_tag="K0W59_003792"
                     /pseudo
     CDS             complement(5131..7373)
                     /gene="nrfB"
                     /locus_tag="K0W59_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
BASE COUNT         1738 a         1939 c         1952 g         1833 t
ORIGIN      
        1 tttccgttag ggcggtaaat tgtactttga tacatgaaaa tacgggtttt cttgattcag
       61 acgcgcagcg gtgtgcgttt gtttgccgct atagcgaaat aaatcagaaa atcagacgcg
      121 gtcgttcact tgttcagcaa ccagaccaaa agccattgac tcagcaaggg ttgaccgtat
      181 aattcacgcg attacaccgc attgcggtat caacgcgccc ttagctcagt tggatagagc
      241 aacgaccttc taagtcgtgg gccgcaggtt cgaatcctgc agggcgcgcc atttcttctt
      301 catttatgcc cgtcttatcc gtttccgctt tgcccttcac cacatcactt tttgtcgctg
      361 tttggcgaga taattcaacg gtgtcaggct gtaaaacgcc ttaaaaacag agataaagta
      421 cgacgtgctg ctatagccac ataattgcgc cacctgagtg atatttttgt tatccatcaa
      481 taacatctgt acggcgtaac gcatacgaca ctctgtgaca atctggctat agctggtatt
      541 ttcgtttttt aatttctttt tgagcaggct ggggcttaaa catagcgaac tggcgacaat
      601 tcgcagattc cagtaatgct gaatatcgct ttgaataatg cggcagacgc tgtcgcggac
      661 gctgctgcgt aagatataca tcagtagggc aataaaccgc gattgctcaa gaaagttaga
      721 caatacggta aaaagtaatg cgcgcgtcaa ctccgcttcg cagggtttgc tgggttgctg
      781 cttgctgtgg ttggcggcta gccggaacac ttccggtgta cggctatgac aagcgataac
      841 cggggttgcc agccgcgacc agggctttac acaggttaat ttcttattta aaaacaacag
      901 ataatcgttg atgatgttac ggttgatgtg ggtgattaat acgtgattta acgttgagag
      961 ttcaatgacg ttattgttgc acgcgaaggc ggccaggtag tttttcggaa gggtaaacgt
     1021 agcatggtta ttgacgctta cctctacctc tttttcggtc aggatcacca cgcaaggttc
     1081 actgctgctc aattgcattt cgcactcctc agatatcaga aactccgctc aaaggatcta
     1141 tgcttcctgc atgagtgatc ggcccgttcg ccgataacga tcttctttct ttagcatgct
     1201 ttttagcaat taatcttgat ggaattctga cgagagcgaa agaggtaagc caggtcgtac
     1261 ccgacttacc tggaggggat ttaatactcg agaatgccgt gcgcgacggg caaatagcgc
     1321 agagagaaat agagcatgtc tttaccgcaa gaggaacgtt tcgctaacag cgaggcaatt
     1381 tctgcgtccg gtttcggttt cgctgtaaag gttttgcctt tgccggcgac aaacagttca
     1441 taaacaatgc cgcttgctgg ggcggagctt tggcaatcgg cgctggcctg tagataacgt
     1501 tcacgctgtt cagcggtaag tttatccacg ccgctgccat cctgtaccca gacattaact
     1561 ccggttgctt taacttgttc cagcagttgg cgatagccat cgggcgacat gtttccggcg
     1621 aaaaaactac tgatataaac cggttttgct gaaacatcgc taatcagccg ctgcgcgttg
     1681 tttaaccatg ttagcaaagg ttgacgggcg gcttcgctgc gccagttcag gtcgtcaatt
     1741 tccgcgctga tgtaccagcc atccggcgtt atgccaggcg cggcgctcca taatctggct
     1801 tgctggagat cggcagccag caggcgatta agatagcttt ccagcgctgc agacgactgt
     1861 ttctggtgca taaaaaattc cggatcggcg ttcagcccga caatcagctt caggccagtc
     1921 tgttgcgcag ctgcggcacg cttaaacaat aacgtgcgct gttctggctg ggtaaatgca
     1981 tcgccgtaac gggtccattg caaaaccagg gtatcgaagc cttgaaaacg taactgactc
     2041 atcagcccct gccactgggt atcggtaacc tgactatctc ggttttgtgg ttgccagata
     2101 atacctttca tcgcaaagga aaaagggctg accaaaagca gtgtcagcaa tacgaaaatg
     2161 aacttacgca tttaccagtg cactccaatg gtgagaaacg cgttgttgcg ctctccgtta
     2221 cgttgattaa tcgccttaaa ggtatgttga tactcgacgc cgagactgac tttgtgcggc
     2281 caggcgtcgt agtgcgtctc gccggtccaa atattccagc ggaccccgac accgccaagc
     2341 tgcgcgccct gagtgccttt atcacgatag ccgttgtcct gaacgtgagc gtaaggctca
     2401 atagtctgtc cgttagctac cttctgatgc cagctgacgc gataatctgc cgtccacgcc
     2461 tgaatatcct ggcggatata ttgcgccgca tcgaggtaca ggttttgggc aaaccagcct
     2521 gaaccgttcg ggtgccattc gtcgctgtat ttgccgccat taaagaatga ggcgctggcg
     2581 cgcagcatgg tatcggatgc gccattttgc ccgttcagcg gcaactgctg ttcgacggcg
     2641 aggaaaaaga tctgatcgcg cagcggcttc cagcgcagac cggtgccgga catcggatct
     2701 ttcaccggca tcatcacccc gttttctccg gtatcggcaa agacgcggct ataaactgag
     2761 agcaggtcgc cttccagcag catattgcgt ccgatgcggt actcggcttc cagttgtccg
     2821 tagctacgat agcttttccc aggcgctgca ccgccgacat tattgttagc ggtactcatt
     2881 gcgccggaac gcaagccgat ggaagaatcg aaactgaacg tccagcggcg accgacctcc
     2941 tcatgcaaac ggcggaaatt gaagcgttgc tgattttgct ctggggtcag tggggttatc
     3001 agcgcctgat tatcaatgtc atcaatcacc agccgggcgt agtgctgcgt cgcaggcatg
     3061 tcatccagac gctggttcac gtaggccagt tgtcggatca gtgccggatc gtccggaagc
     3121 cctttatgcg cctgttcgag catttcccgc gactgtgcga tatcaccgct atcccacaag
     3181 gcgtaaccaa gcgctgcctg ggtgttgcta ttattcggtt ccagttccag cgcggcgcgc
     3241 aaatcactca ccgcggccgg aacattatga cgttggcgat aaattgtcgc ccgcgcaacg
     3301 taagcgttgg cagaaggggc aatattgatt gagcgcgtga gatcgttcag tgcgagttct
     3361 ggctgagctg ggatataacg ttgcgcatgc agccaccagt attgagcatt atttcccagc
     3421 tcacgttgtt ctgcctgttg aagccagcga tcgcgagccg cgccatttcc cgctgcctga
     3481 gcggtgttgg cggcggcgag cagatcctca ttgctcatgt cgtgaagact gattttctgc
     3541 caggccgcca gtgcggtggc gtagtcctca acctgatacg cctgataggc taccgcacga
     3601 tgttgccagg cgctcggttg tcgttgttcg gcctgaagcc atgcatacaa cgccacaccg
     3661 ggtagcgtgt cccgataaca ctttgccaga cggttccagg cggcagcatc gtaggaaggc
     3721 gacatatcgc ccagcaagcg aactattgcc gggcaattat ctgcaatacc cggcaactga
     3781 ctttgccact gacgttgctc cgccagcggt aagggtttcg ataaaatcgc caccttcgcc
     3841 ggcgttgcta ggtaaggatg actttccagc agagacgcca gtcgcgccat taaagtctgg
     3901 ctgacacgcg catcgccctg gaaaggatag cgttgcagca ataaatcggc agcttcgcgt
     3961 gactgctcgt tctgcatcag ttgccaggtc agttgatcca ggcggggaag atttgtcggt
     4021 tcttgctgat acagcaatcg tgccagacgc agagattcag ccttgttacg ggtcgccacg
     4081 ctgacagcat aacgctcctc aagcatttca ttggcgggga gggtggcgag cagtttttgc
     4141 gctgcgtcgt actgaccttc ttttaacagc accggtagcg tcgcgccaac aacatactgg
     4201 cggttatcgg caaactgcac cgtataattc gccaatgcct gaacggggtt ggcgctgtat
     4261 ttagataaca gatagagcca acttttctct tgtgcgcccg tggtaaacag cggcttattt
     4321 tcaatgagat aatgctggag gcgtgctttt tcgccacgat aagccagcgc ggtcgcgtaa
     4381 gtaatatatg actgaggatc ggtgaagatc ccctgtgatt gcagtgccag gatccgatcg
     4441 tccagctgcc cggcaagaag cacgtcaaac cactgacggc gttctgccgc gcttaatgtg
     4501 ttctgctggc gtgcttcatt gtatagcgta tctgcctggg accattgttt caggtagatt
     4561 gcccgttgca gcagatcggt tcgcagcgtt tttccttccg gcgatgcagc aaacgtcgca
     4621 tcgttcagtt gcgctctggc gacaggtaat tgtgccagcc gcagggcatt ctgcccgact
     4681 tcactgcgac aacgcagggt cggcgcagcg tcgcccgctt tttgctgggc aagcagttct
     4741 tcaacagtcg taacgctttt cacttcaact ggaatagccg ccagactgcg ctcaagtcgg
     4801 gcatctcctg ggtgacgttt cagttgatcc tcaagcaaca gccgcgcccg gtcatcatga
     4861 ccaaaatgac gataggcttc cgcaaggtat aaagtcagcg gaatattatc cggcacctgt
     4921 tggtgtatat attcaaattc gcggatggcg gttgcttcgt cgttattttt ctgtgccttc
     4981 agcgccttat cgagacgggg ataaataaca aaatggcgat aatcgctcag ccccagctct
     5041 tctgcgctgg tgccgatatt gtctgcgagt gcgctggtac tcaataaaga cgtcagcagt
     5101 aaaccagacc atccgatgac gcgattaagg ttattctcct tcattttcgg actccagttg
     5161 cgcaacctgt tctgtgttta aacctgcttt gagtaatagt gattgcatcg aaacttgtaa
     5221 ttcgcgttga attgtcagga cgcgatccaa cgtttcctgg ctgataacgc cttcggtgac
     5281 caaaaacttg ccgagcggca gagaactgcg ttcatggcgc aataacaaca cgttaattgc
     5341 tgaacgatta atatgaccga gcgtggtcag tatttcggcg aacaggaact gatgcggcac
     5401 atattgccgc cagatttcac cggtctgctg ttccgtgagc cactgatgct gaaccgcatt
     5461 gtacaacatt gcccgcggat cgtgaccgcg tcggcgtgca taccagtggc gtaaccctgt
     5521 gacaatttgt ccccgcagaa caatgacgta acgcactttg cgtccgactt tacgcgtcag
     5581 ggccgccagc gaaaccgggt caataccatc ttcactgccg acaattaact catcattttc
     5641 cagacgcagc ggcagtactg cataatgcag cgctacggag gctggcattt cggcaatcag
     5701 cgaggacggg atctgccagg catcgatgga ttcccacgcc acgccgtttt gctctgcctg
     5761 cgcctgtgcc agctgctcgg cgctaatcag cccctgcatc agcattgaac cgcccaggcg
     5821 tagaccttcg acgcgattac gcagtgctgt atcgagttgt tcttcagtga tgacctgatt
     5881 ttccagcaga atttgaccta acgggcgcaa cgagcgggta tcgccagtga cgctggggaa
     5941 gtcatgcgtt gttttatccc acgccacgcc acgcgacgcg gatcgccgtg ctgaagaacc
     6001 tgtttcagca cgcgccagtt agccataaag ttaatcaggt tgccccagaa aagacgcagg
     6061 acggaaagca ggccctgcgt gaggccgtag tagccagtga cgaaaatcac tcgctgcacg
     6121 atgcggttaa ccatcaagcc aaagtttagc cacagcaggg tcattaacca tgcgctgccg
     6181 ctaaaaatag aaaggaaatg ccaggcatcg ggccacaaac tttcatacgc cagcaacagc
     6241 aaaagctgga tcatcaccag catcgcgagg aagctgacaa agttactgat tgcccctttg
     6301 cggtcgcgcc agagaaagta gttcagcgtc aggctggagg tccatttatg ggttttaaag
     6361 ccctggaaaa caatgccgat gatccagcga gatttttgtc gaaccgcagt cgaaaaggta
     6421 tcggggaaat attcgcgcac gcagatcatg tttgacgtcc gtgcgtgctg taaaaattta
     6481 cgctgctcgc gttctttggc ttcgtccacc accggaaaac ggacaaaaat ttccgtcata
     6541 cctttttctt tcaggcggaa gccaatgtcg taatcttcag taagactctg cacgtcgaaa
     6601 gcaataccgt taccgtcagc taacagtgcg gtcacggcgc ggcggctgaa acaggtgccg
     6661 acgcctgcgc tgggcacttg tccggcgagg gcttcacgca ccggaacatc tttgccatgc
     6721 agttctgaaa actcatcaat gtaagtcatg ctggtgaagt gcgtccattc gcgttcaaac
     6781 ggatacactg ggatctgaat cagatcttta cgctcgacca gatagttgaa cagacgcaat
     6841 tccatcggtg aaatcacatc ttcggcgtca tgcagaataa aaccagcaaa agcgaaattg
     6901 gcgctacgtt caaactgggt gatggcgtcc agcacgttgt tcagacagtc ggctttgctg
     6961 gtgggaccag gacgcgcgca gactacctta tgcacgttcg gaaagcgagc gcacacttcg
     7021 tcaacatcac gttgagtatc ggggtcgttg gggtaggtgc caacaaagat atgatagttt
     7081 tcgtagtcga gcgtggtcgc cgccagctcg gccatattgc cgatgacgcc cgtttcattc
     7141 cacgccggaa ccataatcgc taacggtttt tcatctggtt tatacagttc gcggtaactc
     7201 attcgcgggt agcggcgata aacactcaac ttgcgtttaa tgcggcgtac ccagtagacg
     7261 acatcaataa aaaaatcgtc cagcccgctg atgaacatga tgaccgctaa cgttatcgcg
     7321 attactttta agctgtagag ccaggtagca aaaacatcaa gaagccagtc cacacaaaaa
     7381 ccttacatta acgctggtta tgtttagggt ggcgtatatt aaggtttttt atgaattgtg
     7441 acagcttttt accattaata gg
//
LOCUS       DAETPZ010000146         7401 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.148, whole genome shotgun sequence.
ACCESSION   DAETPZ010000146 DAETPZ010000000
VERSION     DAETPZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.148"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..233)
                     /locus_tag="K0W59_003793"
     CDS             complement(<1..233)
                     /locus_tag="K0W59_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HBI3024634.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSFGFAPIFP"
     gene            753..1448
                     /gene="minC"
                     /locus_tag="K0W59_003794"
     CDS             753..1448
                     /gene="minC"
                     /locus_tag="K0W59_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBI3024635.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAGRL
                     QLVENALTVQPLN"
     gene            1472..2284
                     /gene="minD"
                     /locus_tag="K0W59_003795"
     CDS             1472..2284
                     /gene="minD"
                     /locus_tag="K0W59_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBI3024636.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2288..2554
                     /gene="minE"
                     /locus_tag="K0W59_003796"
     CDS             2288..2554
                     /gene="minE"
                     /locus_tag="K0W59_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBI3024637.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2926..3270)
                     /locus_tag="K0W59_003797"
                     /pseudo
     CDS             complement(2926..3270)
                     /locus_tag="K0W59_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3305..3424)
                     /locus_tag="K0W59_003798"
     CDS             complement(3305..3424)
                     /locus_tag="K0W59_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBI3024638.1"
                     /translation="MPLSYAGYHHFIMMSLVSAEHNTPALSLQDESPGLVPAG"
     gene            complement(3385..3570)
                     /locus_tag="K0W59_003799"
     CDS             complement(3385..3570)
                     /locus_tag="K0W59_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024639.1"
                     /translation="MSRRYSSATITLVILFCRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3671..3844
                     /locus_tag="K0W59_003800"
     CDS             3671..3844
                     /locus_tag="K0W59_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024640.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3846..4190
                     /locus_tag="K0W59_003801"
     CDS             3846..4190
                     /locus_tag="K0W59_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HBI3024641.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4194..4529
                     /locus_tag="K0W59_003802"
     CDS             4194..4529
                     /locus_tag="K0W59_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HBI3024642.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4586..6907)
                     /locus_tag="K0W59_003803"
     CDS             complement(4586..6907)
                     /locus_tag="K0W59_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBI3024643.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINSQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LYLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTETTNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(7024..7233)
                     /locus_tag="K0W59_003804"
     CDS             complement(7024..7233)
                     /locus_tag="K0W59_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024644.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
BASE COUNT         2032 a         1742 c         1647 g         1980 t
ORIGIN      
        1 tctggaaata taggggcaaa tccaaatgat tgtaagtttt cagcggcttt ttcgccgaga
       61 tatttccttg ttaactctgg ggaaatgcca tctgagttca cacaaatata cgggtcgcaa
      121 acaacgccgc acgccggaga ttgaagtccg ccagcgtcat gtccagacgc cattgatgag
      181 aacatcatcc ccacacctga caatccgata taaataatac gcatcatatt catagcacat
      241 cctgataatc caaccgtaca acacttatgc tcatttcgtc atcaatccgt aatcaaaacc
      301 gctgacctgg acgagttacg atgacctgac agaagtatca gacgtcttct gcggtttcta
      361 tgatagagca attctgtggc ggatattaag ataacgggta tctgttcttt aatgatgctg
      421 caaaaaatgg aaagttaaga aagcctggcg tggtgaaaaa atacacttgc ctcaatactg
      481 gatatttaaa caggtctgga gatgctgtca cgattagggt gcggaaaaag gaagcgtaaa
      541 taagaagaat caaaagtacg acggcaatgg gttgattgac aagggtattt tttaagctat
      601 gaatcagcgc catttatcac agaatagact tttactctga ataaatggga gggtgacttg
      661 cctcaatata atccagacta taacatgcct tatagtcttc ggaacatcat cgcgcgctgg
      721 cgatgattaa tagctaattg agtaaggcca ggatgtcaaa cacgccaatc gagcttaaag
      781 gcagtagctt cactttatct gtggttcatc tgcatgaggc agaacctaag gttatccatc
      841 aggcgctgaa agacaaaatc gctcaggccc ccacattttt aaaacatgcc cccgttgtac
      901 tcaacgtcag tgcactggaa gacccggtaa actggtcagc gatgcataag gcggtttcgg
      961 caaccggttt gcgggttatt ggcgtaagtg gctgcaaaga tgcgcaactt aaagccgaaa
     1021 ttgaaaaaat ggggctgcct atcctgacgg aaggaaaaga aaaagcgcca cgtccagctc
     1081 ccgcaccgca ggctccagcg caaaatacaa tgccggtcac aaaaacgcgt ttaatagata
     1141 ccccggtgcg ttccggtcag cgtatttatg ctccacaatg tgatctgatt gttacaagcc
     1201 acgttagcgc tggggccgaa ttgattgccg atgggaacat tcatgtctat ggcatgatgc
     1261 gcggtcgtgc gctggcaggg gcaagtggtg accgggaaac gcaaatattt tgtacgaacc
     1321 tgatggcgga attggtgtcc atcgcaggtg aatactggct gagtgatcaa atcccagcag
     1381 aattttatgg caaagcgggg cgactgcagt tagtcgaaaa cgctttgacc gttcaaccgt
     1441 taaattgatc cctttttaac aaggaatttc tatggcacgc attattgttg ttacttcggg
     1501 taaagggggt gttggtaaga caacctccag cgcggccatc gccactggtt tggcccagaa
     1561 gggaaagaaa actgtcgtga tagattttga tatcggcctg cgtaatctcg acctgattat
     1621 gggttgcgaa cgccgggtcg tttacgattt cgtcaacgtc attcagggcg atgcaacgct
     1681 aaatcaggcg ttaattaaag ataagcgtac tgaaaatctc tatattcttc cggcatcgca
     1741 aacacgcgat aaagatgccc tcacccgtga aggggtcgcc aaagttcttg atgatctgaa
     1801 agcgatggat tttgaattta tcgtttgtga ctccccggca gggattgaaa ccggtgcgtt
     1861 aatggcactc tattttgcag acgaagccat tattaccacc aacccggaag tctcctcagt
     1921 acgcgactct gaccgtattt taggcattct ggcgtcaaaa tcacgccgcg cagaaaatgg
     1981 cgaagagcct attaaagagc acctgctgtt aacgcgctat aacccaggcc gcgtaagcag
     2041 aggtgacatg ctgagcatgg aagatgtgct ggagatcctg cgcatcaaac tcgtcggcgt
     2101 gatcccagag gatcaatcag tattgcgcgc ctctaaccag ggtgaaccgg tcattctcga
     2161 cattaacgcc gatgcgggta aagcctacgc agataccgta gaacgtctgt tgggagaaga
     2221 acgtcctttc cgcttcattg aagaagagaa gaaaggcttc ctcaaacgct tgttcggagg
     2281 ataagttatg gcattactcg atttctttct ctcgcggaag aagaacacag ccaacattgc
     2341 aaaagaacgg ctgcagatta ttgttgctga acgccgtcgc agcgatgcag aaccgcatta
     2401 tctgccgcag ttgcgtaaag atattcttga ggtcatttgt aaatacgtac aaattgatcc
     2461 tgagatggta accgtacagc ttgagcaaaa agatggcgat atttctattc ttgagctgaa
     2521 cgtgacctta ccggaagcag aagagctgaa ataagcccgc tgtaaaaacg catttatctt
     2581 caaggcagag tcatttctgc cttgagtttt tcatccctct catccacgtt gtggtaaagc
     2641 ggcgagtatt cttgctgata ctcctcattg ctatttccac ctcccccttt acaccttaag
     2701 gctgttccca cgcacagaac aaaatgttta tgcctggttg agtaaataac catgttgtta
     2761 gtatggatat gctgggagat gataaccccg caatttcatt attcattgtt ctcccatagg
     2821 atgaggtttc ccgccgtaac tggcgggctt tttttgcccc agaattttct ctttcaacat
     2881 cctgtaaacg aaaactgcgc cgaagcgcag tttaatcgtt gatggtcaga acgaccagtt
     2941 cacaccagcc accgcgttcc acggggactc cacaccggca ccatggctat accccacccc
     3001 gaaatgcccg cttaacgtac tgctgaatga ggctttaata cctgcctggt atattccacg
     3061 tctgcccgac aaatcattga caaaattacc gtcactattc actttcaccc gattatcatc
     3121 gacaaattct ttgcgcacag ccgccttcag gcagggttca atttccatac cgttccccag
     3181 acgcatgttg taactcagcg ttgcacccag ttcacgatat atactgcggg tatcgactga
     3241 tttcgattcc atgccattgg ataaatgata gtagcgtcta agtgacctaa gctctgctgc
     3301 aacatcagcc cgcaggcacc agaccagggg attcatcctg aagagacagc gcaggtgtat
     3361 tgtgttcagc gctcaccaaa gacatcatga tgaaatgatg atatcccgca taagaaagag
     3421 gcatttttta cacgcagtgc gctgaagtgt agttggataa aaaatcactc ttttcagata
     3481 atactgtcgt acgcttatct ttagacaatg aggccgtcgg caaaataaaa tgactaatgt
     3541 aatcgttgct gatgaatatc ttctgctcac ataaaaatca cacaataata ttgataaatc
     3601 agattatctt acctttacaa cattgcgtta acagtcaatg acacttcttg aataaaaata
     3661 ttgtcaatca atgagttatt cctcatttaa gattattctc attcacgtaa aaaacataat
     3721 tcctatcatc acagccacgc tcattttgaa ttatctgaat aatagcgagc gttctttagt
     3781 taagcagatc ttaattgaag acgaaattat tgtgtgtgca acttacttaa taccggacat
     3841 ctaagttgaa gaaaaaaata cttgccttcg ggcttatctc agcattgttc tgctccacgc
     3901 cagcgatggc ggatatgaac cgcaccacga aaggtgcatt gctaggcgca ggcgttggtt
     3961 tacttactgg caatggtgtt aatggcgtac tgaaaggtgc agctgttggc gctggtgttg
     4021 gtgcagtaac agaaaaaggc cgcgacggta aaaatgcgcg taaaggtgca aaagtgggtg
     4081 ccgccgttgg tgctgtcact ggcgttctga ccggcaatgg cctcgaaggg gcgattaaag
     4141 gtgccgtgat tggcggtacg ggtggtgcaa ttctgggtaa aatgaaataa ggaatgatga
     4201 tgaaaaaatt tgctttactg gcgggcctgt ttgttttcgc accaatgacc tgggcgcagg
     4261 actacaatat taaaaacggt ttgccgtcag aaacctatat tacttgcgca gaagccaacg
     4321 aaatggcaaa aactgacagc gcacaggttg cagaaattgt tgcggtaatg ggtaatgcca
     4381 gcgttgccag ccgtgattta aaaattgagc aatctccgga actgagcgcc aaagtggtag
     4441 aaaaactgaa tcaggtttgc gctaaagacc cgcaaatgct gttgatcacg gcaattgatg
     4501 acaccatgcg tgctatcggc aaaaaataat atttcatatg taccgcgtcg atgaatacga
     4561 cgcggttttt tagctaacgg cgggatcaga acttatattt caggcccacc atcactgcgg
     4621 tgtcattgta gccattatcg cccagctgca cgcccacatt cccccagacg ctggccgctg
     4681 gattcaggtt gccatttacg ccaagtttaa tttcccccaa attactcacc ccatcttgtt
     4741 cgactttcac accgttcatt gagacagcaa aatctttact gttatggatc cagttcattt
     4801 caacaaaagg ttcaaagtcg tcaccgtgcg gaccgctgtt tttctcctga gtacgaataa
     4861 aggtgcggaa gccgagtcgg gtctggatgt tgttatttgc gtcattctca atacgtgagc
     4921 cattggcttc cgtgtgcgca ttatgatcga cgcccatcca gatagcctgt gcctgtggct
     4981 ggaagatcca cgtatatttc gccgcttcca gaccaaagga tttattcagg gcaaaaccgt
     5041 aacccgcttc cagcgagacg gttgcacctt tcgcgctata ggattcatag gctaattcat
     5101 cccctttcac ggagttttta aaccagctat attgcgccca ggagtcaatg tatgcgcctt
     5161 ttttactgat gtcatcggca aaccaagtgg catacagccc tgcgctatag cctctgacac
     5221 tccctttcga acgataatcc gacacgctgg aatgagttaa gttgtagtcg cgggcataac
     5281 cagccatcac tcccaaacgc caactgtcac tatcggtaaa accaccggtt aacagatcgc
     5341 cccctaactg cgagacgtag cgatgcgagg tcgttctcaa ctgtccgttg ctgtcacgcc
     5401 aggcattatg cccgccaatt tgacgtagcc aaagtcggct tgaacgctcc tgttcagtta
     5461 caggatcgat gtagcgcgtt tcacccgcac ggtcgttcag acgcataaca aaaagagtgt
     5521 tagcggctgc caggttggcg acgtaacttc cagcttcggg gcgcaggacg ggggtaggcg
     5581 caacagtagg cgctgaactt cctccggagt ttgtttcacc gccgctcgtt gcctggaagt
     5641 tagtcagata ccagttttta ttggtcgttt cggtattacc tcgggttaat gaatattcgt
     5701 aggcaccggc gaaaatcctc gaatccttaa taaattcccc atttgattcc ccctcaacgc
     5761 tgataatctc tatcccattt aacgtgtaag ctccgctgcc tcctgcattg gtaactttaa
     5821 ctcgggttgt tccagaggta tttccattaa taacgacttt atccgtagct gaattatcat
     5881 catccaattc agttctgaga tacaatacgc cattattacc aacgtaattt tcagttattg
     5941 ttaatcttgt tggttcgaca tcccgtccat ctgcccggga aatatagacc gtactattat
     6001 caaccgtgag gttgctcacc gttgagtcag ctttaatatc ccaggtacta ttatctgaaa
     6061 gcgacagata ggtatgagtg ttatcatctg ttgaaatatt agctgaaccc gttatttttg
     6121 aattagttgc atcgacggtc aaatgacctt taatattatt gataaaaaca tctgcagccg
     6181 atgatacctc actatccgta atggtcacgt tactcgtctc atttttatgg tagaggattt
     6241 catttgctga ccgtagtgag ctaccagata aaatatctac cgttgcttcc tgactattta
     6301 tggaagactc cagcacaatt ccagacccct gatgcgcgac tgcagatgta tttttcagcg
     6361 tcaccgtgcc gccattataa attttagcaa tcgcggcgtc aggacccgtt gtataaattt
     6421 ctccctggtt aaatgaaact gtcccctggc gggcagcgat ggcatgagca ttgtccccat
     6481 gcgtggtgat atcaacatcg gttaatgtaa cgctggcatt ggtataatca agcgtgtcac
     6541 cagccttgac gacagtgcca ttagcgtacg cggcataact ttctgttccg tgggttgtga
     6601 tctccccttt tgccatatcc agttttgcta tggcattatt ttcattactt cctgtatcgg
     6661 cagtactttc ttcatcaaga gaagatacac acgcacgcgc gcttccggga gatgcacacg
     6721 caagaacgcc gactgcgtga ttacccgcag taacaataaa tgtatcatct ccgtcagaaa
     6781 aagagggggt aaccgaaccg tcagagttga ctcttattat ggtgtcagta ttcaggtttc
     6841 cactcgtttg acttagcaaa ccaattgagt aatcccccgt tgtgacaatc gtgtttttac
     6901 cctgcaaaat aacagcaccg ccaaattcac ttacgattgc gctagctgtt aatgctgggc
     6961 ttagaggact attgtcagaa ggatataaac cgcccgttgt tacaatgacg ccattgttaa
     7021 tggttaactt actaccctct cctgcaacgg atacccgtaa cttccattac ccgttgttat
     7081 aactagcgca ttatcaatat cttttacttt tccatctttt attgccacag catgtttata
     7141 ttcatcgtgc tgaataaact gttcagcata tgcattaccc accgccagag caataagtcc
     7201 caaactaaac ccggggagtt ttttcagttt catagattaa ccatccattc attggattaa
     7261 caaacattcc tggtcaaata acctatgagc tcagattacc aaaacggagg gtaaagacaa
     7321 aaacaaaagc acaaaaaatt taaatagata aacagcgaca atatataaat gataaataag
     7381 tcggcattat catttttcac c
//
LOCUS       DAETPZ010000147         7297 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.149, whole genome shotgun sequence.
ACCESSION   DAETPZ010000147 DAETPZ010000000
VERSION     DAETPZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.149"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..1153
                     /gene="rbsA"
                     /locus_tag="K0W59_003805"
     CDS             <1..1153
                     /gene="rbsA"
                     /locus_tag="K0W59_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HBI3024645.1"
                     /translation="YVEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFESKV
                     IIMDEPTDALTDTETESLFRVIRELKSQGHGIVYISHRMKEIFEICDDVTVFRDGQFI
                     AEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLRKG
                     EILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISEDR
                     KRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQAIG
                     LLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIILVS
                     SEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAVVGKLNRVNQE"
     gene            1158..2123
                     /gene="rbsC"
                     /locus_tag="K0W59_003806"
     CDS             1158..2123
                     /gene="rbsC"
                     /locus_tag="K0W59_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBI3024646.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2148..3025
                     /gene="rbsB"
                     /locus_tag="K0W59_003807"
                     /pseudo
     CDS             2148..3025
                     /gene="rbsB"
                     /locus_tag="K0W59_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3151..4080
                     /gene="rbsK"
                     /locus_tag="K0W59_003808"
     CDS             3151..4080
                     /gene="rbsK"
                     /locus_tag="K0W59_003808"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBI3024647.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4084..5076
                     /gene="rbsR"
                     /locus_tag="K0W59_003809"
     CDS             4084..5076
                     /gene="rbsR"
                     /locus_tag="K0W59_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HBI3024648.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5042..6469)
                     /gene="mdtD"
                     /locus_tag="K0W59_003810"
     CDS             complement(5042..6469)
                     /gene="mdtD"
                     /locus_tag="K0W59_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HBI3024649.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     NVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6492..7184)
                     /locus_tag="K0W59_003811"
     CDS             complement(6492..7184)
                     /locus_tag="K0W59_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBI3024650.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDIAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1845 a         1764 c         1929 g         1759 t
ORIGIN      
        1 gtatgtcgaa gcggataaat tgctggctaa acttaacctg cgctttaaaa gcgacaagtt
       61 agtgggcgat ctttccatcg gtgaccagca aatggttgaa atcgccaaag tgctgagctt
      121 tgagtcgaaa gtcatcatta tggatgaacc gaccgatgcg ctgaccgata ccgaaaccga
      181 atccctgttc cgcgtcatcc gcgagctgaa atcgcaaggc cacggtattg tctatatctc
      241 ccaccgcatg aaagaaatct tcgagatttg cgatgacgtt accgtttttc gtgatgggca
      301 atttattgct gagcgcgaag tggcatcact gaccgaagat tcgctgattg agatgatggt
      361 gggccgcaag ctggaagatc aatatccaca cctggacaaa gcgccaggag atatccgcct
      421 gaaagtcgat aatctctgcg gacctggcgt taacgatgtc tcttttactt tacgcaaagg
      481 cgaaattctt ggcgtctctg gtttgatggg cgcaggtcgt accgaactga tgaaagtgct
      541 ctacggcgca ctgccgcgca ccagcggtta cgtcaccctg gatgggcatg aagtcgttac
      601 ccgttcaccg caggatggcc tggcaaacgg cattgtgtat atctccgaag accgtaaacg
      661 tgacggttta gtgctgggca tgtcagtaaa agagaacatg tcgctgacag cgctgcgcta
      721 cttcagccgc gctggcggca gtttgaagca tgccgatgaa cagcaggctg tgagtgattt
      781 cattcgtctg tttaatgtga aaacgccgtc gatggaacag gcaattggtc tgctttccgg
      841 tggcaatcag caaaaagtgg cgattgcccg cggtctgatg acacgcccca aagtattgat
      901 ccttgatgag cctacccgtg gcgtagatgt cggcgcgaaa aaagagatct atcaactgat
      961 taaccagttc aaagccgatg gcttgagtat cattctggtg tcatcggaga tgccagaagt
     1021 attaggcatg agcgatcgca tcatcgtcat gcatgaaggg catctcagcg gggaatttac
     1081 tcgtgagcag gccacccagg aagtgttaat ggctgccgtt gtgggcaagc ttaatcgcgt
     1141 gaatcaggag taaaaaaatg acaacccaga ctgtctctgg tcgccgttat ttcacgaaag
     1201 cgtggctgat ggagcagaaa tcgcttatcg ctctgctggt gctgatcgcg attgtctcga
     1261 cgttaagccc gaactttttc accatcaata acttattcaa tattctccag caaacttcgg
     1321 tgaacgccat tatggcggtc gggatgacgc tggtgatcct gacgtcgggc atagacttgt
     1381 cggtaggttc tctgttggcg ctaaccggcg cagttgctgc atctatcgtc ggcattgaag
     1441 tcaatgcgct ggtggctgtc gctgctgctc tcacgttagg tgccgcaatt ggtgcggtaa
     1501 ccggggtgat tgtagcgaaa ggtcgcgtcc aggcgtttat cgctacgctg gttatgatgc
     1561 ttttactgcg cggcgtgacc atggtttata ccaacggtag cccagtgaat accggcttta
     1621 ctgagaacgc cgatctgttt ggctggtttg gtattggtcg tccgctgggc gtaccgacgc
     1681 cagtctggat catggggatt gtcttcctcg cggcctggta catgctgcat cacacgcgtc
     1741 tggggcgtta catctacgcg ctgggcggca acgaagcggc aacgcgtctt tctggtatca
     1801 acgtcaataa aatcaaaatc atcgtctatt ctctttgtgg tctgctggca tcgctggccg
     1861 ggatcattga agtggcgcgt ctctcctctg cacaacccac ggcggggact ggctatgagc
     1921 tggatgctat tgctgcggtg gttctgggcg gtacgagtct ggcgggcgga aaaggtcgca
     1981 ttgttgggac gttgatcggc gcattaattc ttggcttcct taataatgga ttgaatttgt
     2041 taggtgtttc ctcctattac cagatgatcg tcaaagcggt ggtgattttg ctggcggtgc
     2101 tggtagacaa caaaaagcag taataacgac tacaggacat cttgaatatg aacatgaaaa
     2161 aactggctac cctggtttcc gctgttgcgc taagcgccac cgtcagtgcg aatgcgatgg
     2221 caaaagacac catcgcgctg gtggtctcca cgcttaacaa cccgttcttt gtatcgctga
     2281 aagatggcgc gcagaaagag gcggataaac ttggctataa cctggtggtg ctggactccc
     2341 agaacaaccc ggcgaaagag ctggcgaacg tgcaggactt aaccgttcgc ggcaccaaaa
     2401 ttctgctgat taacccgacc gactccgacg cagtgggtaa tgctgtgaag atggctaacc
     2461 aggcgaacat tccggttatc actcttgacc gccaggcaac gaaaggtgaa gtggtgagcc
     2521 acattgcatc tgataacgta ctgggcggca aaatcgccgg tgattacatc gcgaagaaag
     2581 cgggtgaagg tgccaaagtt atcgagctgc aaggcattgc cggtacatcc gcagcccgtg
     2641 aacgtggcga aggcttccag caggccgttg ctgctcacaa gtttaatgtt cttgccagcc
     2701 agcagatttt gatcgcacca aaggtttgaa cgtaatgcag aacctgttga ccgctcatcc
     2761 ggatgttcag gctgtattcg cgcagaatga tgaaatggcg ctgggcgcgc tgcaaactgc
     2821 cggtaaatcg gatgtgatgg tcgtcggatt tgacggtaca ccggatggcg aaaaagcggt
     2881 gaatgatggc aaactagcag cgactatcgc tcagctaccc gatcagattg gcgcgaaagg
     2941 cgttgaaacc gcagataaag tgctgaaagg cgagaaagtt caggctaagt atccggttga
     3001 tctgaaactg gttgttaagc agtagtttta atcaggttgt atgacctgat ggtgacataa
     3061 atacgtcatc gacagatgaa cgtgtaatat aaagaaaagc agggcacgcg ccaccctaac
     3121 acggtggcgc attttatgga catcccgaat atgcaaaacg caggcagcct cgttgttctt
     3181 ggcagcatta atgctgacca cattcttaat cttcaatctt ttcctactcc aggcgaaacc
     3241 gtaaccggta accactatca ggttgcattt ggcggcaaag gcgcgaatca ggctgtggct
     3301 gctgggcgta gcggtgcgaa tatcgcgttt attgcctgta ctggcgatga cagcattggc
     3361 gagagcgttc gccagcagct cgccactgat aacatcgata ttactccggt cagcgtgatc
     3421 aaaggcgaat caacaggtgt ggcgctgatt tttgttaatg gcgaaggtga gaatgtcatc
     3481 ggtattcatg ccggcgctaa tgctgctctt tccccatcgc tggtggaagc gcaacgtgag
     3541 cgtattgcca acgcgtcagc gttattaatg cagctggaat caccactcga aagtgtgatg
     3601 gcagcggcga aaatcgccca tcaaaataag actatcgttg cgcttaaccc ggctccggct
     3661 cgcgaacttc ctgacgaact gctggcgctg gtggacatta ttacgccaaa cgaaacggaa
     3721 gcagaaaagc tcaccggtat tcgtgttgaa aatgatgaag atgcagcgaa ggcggcgcag
     3781 gtactgcatg aaaagggtat ccgtactgta ctgattactt taggaagtcg tggtgtatgg
     3841 gctagcgtga atggtgaagg tcagcgcgtt cctggattcc gggtgcaggc tgtcgatacc
     3901 attgctgccg gagatacctt taacggcgcg ttaatcacgg cgttgctgga agaaaaacca
     3961 ttgccagagg cgattcggtt tgcccatgct gccgctgcga ttgccgtaac acgtaaaggc
     4021 gcacaacctt ccgtaccgtg gcgtgaagag atcgacgcat ttttagacag gcagaggtga
     4081 cgcttggcta caatgaaaga tgttgcccgt ttggcgggcg tttctacctc aacagtttct
     4141 cacgttatca ataaagatcg cttcgtcagt gaagcgatta ccgccaaagt tgaagcggcg
     4201 attaaagaac tcaattacgc gccatcagct ctggcgcgta gcctcaaact caatcaaaca
     4261 cataccattg gcatgttgat cactgccagt accaatcctt tctattcaga actggtgcgt
     4321 ggcgttgaac gcagctgctt cgaacgcggt tatagtctcg tcctttgcaa taccgaaggc
     4381 gatgaacagc ggatgaatcg caatctggaa acgctgatgc aaaaacgcgt tgatggctta
     4441 ctgttactgt gtaccgaaac gcatcagcct tcgcgtgaaa tcatgcaacg ttatccgaca
     4501 gtgcctaccg tgatgatgga ctgggctccg ttcgatggcg acagcgatct tattcaggat
     4561 aactcgttgc tgggcggaga cttagcaacg caatatctga tcgataaagg tcatacccgt
     4621 atcgcctgta ttaccggtcc gctggataaa actccggcgc gcctgcggtt ggaaggttat
     4681 cgggcggcga tgaaacgtgc gggtctcaac attcctgatg gctatgaagt cactggtgat
     4741 tttgaattta acggcgggtt tgacgccatg cgccaactgt tatcacatcc gctgcgtcct
     4801 caggccgtct ttaccggaaa tgacgctatg gctgttggcg tttaccaggc gttatatcag
     4861 gcagagttac aggttccgca ggatatcgcg gtgattggct atgacgatat cgaactggca
     4921 agctttatga cgccaccatt aaccactatc caccaaccga aagatgaact gggggagctg
     4981 gcgattgatg tactcatcca tcggataacc cagccgaccc ttcagcaaca acgattacaa
     5041 cttactccga ttctgatgga acgcggttcg gcatagattt acgctgtctt ttgatcaaat
     5101 tattaccatc ggttgttttc agaagcatga acattgctgc tgaagcaaca gtaataatgc
     5161 ccatcgtgat aaacgtatag tggaattgtt cgacagtcgt tgtgccttcc atcccttcat
     5221 aaatgcgaag gacagccgca cttacagcaa cgcctaaact aatcgacagt tgctgcgtga
     5281 ccgccagaac attgttaccg ctgctggcgt tgtcatcggt cagatcggca agtgtgatgg
     5341 tattcatcgc ggtaaactgc gtcgacatag ccatccctaa tataaacaac ggcaagatca
     5401 gcatccatat cgccattgcc ggtgattgca aagagaactg agcgatcatt agcccaataa
     5461 tcaccgtgat ccccactaat gtatggcgat agcccagacg acgtaagact tgggtaacca
     5521 tcgattttgc aataatggaa cctaacgctg tcggtgccat catacagccg gcaataaacg
     5581 cctgataacc aaatcctacc tgtaacatca atggcataag gaacggtaca cagccggtcc
     5641 ccagacgggt tgcaatattg cctacgatac cgatcgagaa agtgcgggtt ttaaataaat
     5701 ctaatgaaat taatgggttt ggcgtacgtc gcgcatggag aatatagaga agcagtaacc
     5761 cgatgctggt gacaattacc gtcaaggcaa tccagctggc gacaatcttt tccccgaata
     5821 gctctattcc gcttgaaaag agaacaaggc tgaggccaaa cagcaaaaag ccagtgatat
     5881 cgaatctgcg tcgtgcggtg gtgaaattgg gcatatgttt gcgcgcgtaa agaaggcccg
     5941 caatacctat ggggatatta attaaaaata tccagtgcca ggttgcccag gtgaccagca
     6001 cgccgccaag aacggggcct aaaattggcc ccaccagacc cggcatggcg acaaaattca
     6061 ataccggaag aagttcatta cgaggataag cacgcagtaa ggccagccga gcaacaggca
     6121 tcatcattgc gtcgcctatc ccttgaataa cccggaagac aaccagctgt ggtagcgaat
     6181 tagaaagtgc gcaggccaga gaacccaacg tgaacagact cacggcaagg gtaaaaatgc
     6241 gacgcgtacc gaagcgatcg gctagccatc cgcttaccgg aataagcatc gccaccgtca
     6301 gcgtataact gatgatggct gattgcatcg cgagaggaga acgattaagg ctatgagcga
     6361 ttgcgggtaa ggcggtattc agaatagtgg catcaagtgc ctgcatgaaa aaggccatcg
     6421 ccgcgatcca cggcaaaccc gacatactac gcttcttttt atcgctcatt caatgtcctg
     6481 ttatcgggtt atcacttatc aggtgagcgt agcagcgcct gacaagcttt aaatgccgcg
     6541 tcgccatcgc tttggataat cgcatcgaca atcgcctggt gcagatccag ctttatcact
     6601 gtgtcgctgg taattgacgt gaagtaagtg tgataaaccg aatggaatag cgaggcgaat
     6661 gaggtcaaaa acggattggc gctcatttca tagatatgct catgccaggc tatatcgact
     6721 tcgatccagc gttcacggcg aaagttctct tttaatgccg ccatttcggc cattaacgta
     6781 ttgagatgcg ccttctgttc cgcggtgcca accgttgctg ccagtaggca ggcttgcggc
     6841 tccagacaga tacgcataac cagaaagtga tcgatgacct gatgaaagtt ctcttctgtc
     6901 atccaccaag taagcaattc ctgatcaaga aaattccagt tcgattgtgg catgacccga
     6961 gtaccaattc gcggtcgcgg taaaaccatc ccttttgccg ttaacgtttt gaccgcttcg
     7021 cgtaccgctg tacgactcac tccaaattgc tcgcccagct caatctcacc gggcaaaatg
     7081 gtgccgggtt catattcacc ttttaagatc cgttgcgcca gcttctcagc cagaacatac
     7141 gaaaggtttt tctgtgcagc taactgttgt gcgcttaaag gcattactta tcttcctttt
     7201 tctttttatt cctccttagt atgccaccag gaagtgtgat tacggttgca aaaacggcaa
     7261 attgattgtt tgatggcaca ttaacgggac ttttgct
//
LOCUS       DAETPZ010000148         7276 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.150, whole genome shotgun sequence.
ACCESSION   DAETPZ010000148 DAETPZ010000000
VERSION     DAETPZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7276
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.150"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            92..1212
                     /gene="yneK"
                     /locus_tag="K0W59_003812"
                     /pseudo
     CDS             92..1212
                     /gene="yneK"
                     /locus_tag="K0W59_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            1362..2552
                     /gene="ydeA"
                     /locus_tag="K0W59_003813"
     CDS             1362..2552
                     /gene="ydeA"
                     /locus_tag="K0W59_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HBI3024651.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2577..3242)
                     /gene="marC"
                     /locus_tag="K0W59_003814"
     CDS             complement(2577..3242)
                     /gene="marC"
                     /locus_tag="K0W59_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HBI3024652.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3454..3888
                     /gene="marR"
                     /locus_tag="K0W59_003815"
     CDS             3454..3888
                     /gene="marR"
                     /locus_tag="K0W59_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HBI3024653.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3908..4291
                     /gene="marA"
                     /locus_tag="K0W59_003816"
     CDS             3908..4291
                     /gene="marA"
                     /locus_tag="K0W59_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HBI3024654.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            4323..4541
                     /gene="marB"
                     /locus_tag="K0W59_003817"
     CDS             4323..4541
                     /gene="marB"
                     /locus_tag="K0W59_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HBI3024655.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4572..5471)
                     /gene="eamA"
                     /locus_tag="K0W59_003818"
     CDS             complement(4572..5471)
                     /gene="eamA"
                     /locus_tag="K0W59_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HBI3024656.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            5735..6852
                     /gene="ydeE"
                     /locus_tag="K0W59_003819"
                     /pseudo
     CDS             5735..6852
                     /gene="ydeE"
                     /locus_tag="K0W59_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
     gene            6979..7074
                     /gene="mgtS"
                     /locus_tag="K0W59_003820"
     CDS             6979..7074
                     /gene="mgtS"
                     /locus_tag="K0W59_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HBI3024657.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         1898 a         1773 c         1652 g         1953 t
ORIGIN      
        1 agcgccacgc atcacctgcc tttgatagtt cttgcagctc aaatgcccta tatagtgatg
       61 ttccgatgac ttatgactat atggggcaaa tatggttacg ccagtaagca tcagcaatta
      121 catatctctt cctgatgatt ttcccgctcg taatattgca cctcaagtaa aagaagtttt
      181 aaaggatttt attgatgcac tcagtaccat aatatgtgat gaagagtggc gcacgtcttt
      241 aaacaccaac tcagccacaa aaaagatatt taacaacctt gataacttat catatattca
      301 gaggacttct tttcggggta acgacacact atacaatgaa aaggttcagt ttaaactcac
      361 ttatcccgtc aagaatggaa gacacaaaga aaatattgaa tttcaggtag taataaattt
      421 aagccctatt tagataattt tcgccatgat ggagaaatta atattttttg cgctcccaac
      481 ccaaagcctg tcactatggg gcgcgtattt cagaccggcg tcgagcgtgt gctctttctg
      541 tttatgaatg attttattga acaatttcca atgatcaacc ttggcgcccc catcaaaaga
      601 gcgcatacac cacatattga acccctgcct ccggatcacc ataccgctgc agattactta
      661 cgccagtttg atttgcttgt cctgaatttt atctctcgcg gtaattttgt catactcccc
      721 cgattatgga ataactctga ggttcacaga tggttcgtca ataaagatcc taacttgatc
      781 accgccattc tcgacataac ggacagcgaa ttaaaagagg atttattgca aagcctgatg
      841 gattcattag gttctaacaa acatgtacaa cccgaagtct gcatctgctt cttatccctt
      901 ttatcccttt tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc
      961 aatatgttat tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg
     1021 ttgatgccag catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc
     1081 aaactgtttg taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac
     1141 aggcctcgct cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct
     1201 gaacgtcatt aaaccgtgat gttaccgact ctctgacgcg gaacagaatc agcgtcagag
     1261 aaacggaaaa cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag
     1321 atcatttcat cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt
     1381 cccgcaaagt ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca
     1441 ccaccgaatt tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa
     1501 ccgctcaggt cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc
     1561 cttttatgtt aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg
     1621 tgtttattgc cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca
     1681 gtcgcattgg tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga
     1741 tccgtatggc tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag
     1801 cactggcgat ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc
     1861 gaatgacctt cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt
     1921 tacttccctt actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc
     1981 gccgcccggc attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca
     2041 cggcatacag ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg
     2101 ccacggcatt actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta
     2161 aactgggtaa tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt
     2221 gtctggcact gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt
     2281 tctgggggat cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg
     2341 caccagatgc taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg
     2401 gggcgggtgc gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt
     2461 atgtgggcgc ggtgcctgct tttgccgcgt taatatggtc aatcattata tttcgccgct
     2521 ggccagtgac actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa
     2581 tggtacgttt taatgatttc caggatgccg ttaataataa actgcacacc catacatacc
     2641 agcaggaacc ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata
     2701 attgcgccgg agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc
     2761 ggcggcgcaa ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc
     2821 gagctaataa tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca
     2881 aaggcaatat tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt
     2941 gaatcaatcg ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag
     3001 ccgcctgcaa ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca
     3061 gcgtaatacg ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga
     3121 ttacgttcgg cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat
     3181 gggttagcta acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac
     3241 attggtggtt gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag
     3301 gataaagtgt cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta
     3361 gcaaaacgtg gcatcggtca attcattcat ttgacttata cttgcctagg caatattatc
     3421 ccctgcaact aattacttgc cagggcaact aatgtgaaaa gtaccagcga tctgttcaat
     3481 gaaattattc cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt
     3541 aacgagtatc tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc
     3601 cgctgcgcgg cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga
     3661 gcactgaccc gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac
     3721 ccgaatgata agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa
     3781 caatgccatc aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg
     3841 gacgaagttg caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg
     3901 tatgacgatg tccagacgca atactgacgc tattaccatt catagcattt tggactggat
     3961 cgaggacaac ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc
     4021 caaatggcac ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat
     4081 ccgcagccgt aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact
     4141 ctatctggca gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa
     4201 ttactttgat gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt
     4261 tttacatcca ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa
     4321 tcatgaaacc actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagggcg
     4381 ttgcggaaca aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc
     4441 catcgcagga acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg
     4501 atgcgctcgg cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat
     4561 ctcggggctt attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg
     4621 atatacagcc cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca
     4681 tccaacaata gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca
     4741 acccgccagg tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc
     4801 gccacaaacg ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg
     4861 tgaatcatgg atgcggaacc atcgagaatc agcgaggcaa caaagaaggg aatgattggg
     4921 attaaagcgc tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc
     4981 tttttattga agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag
     5041 ccgagcatcg ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa
     5101 atcgctaagg cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg
     5161 ccaagcacga tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc
     5221 ataccaaagt taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca
     5281 taccccaaga gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa
     5341 gcgaccagca taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact
     5401 ttgatgacca caaaatttag cccccatacg accactacca gtagcgccaa caccccatct
     5461 tttcgcgaca ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat
     5521 ttacttttct ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat
     5581 aagtcgctat acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa
     5641 gaagggcgct ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc
     5701 gttatgtttt attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat
     5761 ttacttgagt cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc
     5821 gctcactatt ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa
     5881 gaaacgctat atgttactgg caattaccgc cttcccagcg gttttattgc cattccttta
     5941 gtgaataacg tgacgctggt tgtgctcttt tttgccctca ttaactgcgc ctattctgtt
     6001 tttgctaccg tgctgaaagc ctggtttgcc gacaatcttt cgtccaccag caaaacgaaa
     6061 atcttctcaa tcaactacac catgctaaac attggctgga ccatcggtcc gccgctcggc
     6121 acgctgttgg tgatgcagag catcaatctg cccttttggc tggcagctat ctgttccgcg
     6181 tttcccatgc ttttcattca aatatgggta aagcgcagcg agaaaatcat cgccacggaa
     6241 acaggcagtg tctggtcgcc gaaagtttta ttacaagata aagcactgtt gtggtttacc
     6301 tgctctggtt ttctggcttc ttttgtaagc ggcgcatttg cttcatgcat ttcacaatat
     6361 gtgatggtga ttgctgatgg ggattttgcc gaaaaggtgg tcgcggttgt tcttccggtg
     6421 aatgctgcca tggtggttac gttgcaatat tccgtgggcc gccgacttaa cccagctaac
     6481 atccgcgcac tgatgacagc aggcaccctc tgtttcgtca tcggactggt cggttttatt
     6541 ttttccggca acaacctgct attgtggggt atgtcagctg cggtatttac tgtcggtgaa
     6601 atcatttatg cgccgggcga gtatatgttg attgaccata ttgcgccgcc aggaatgaaa
     6661 gccagctatt tttccgccca gtctttaggc tggcttggtg ccgcgattaa cccattagtg
     6721 agtggcgtag tgctaaccag cctgccgcct tcctcgctgt ttgtcatctt agcgttggtg
     6781 atcattgctg cgtgggtgct gatgttaaaa gggattcgtg caagaccgtg ggggcagccc
     6841 gcgctttgtt gatttaagtc gaacacaata aaaatttaat tcagccttcg tttaggttat
     6901 ctctgctaat atctttctca tcgagatgaa aattaaggta agcgaggaaa cacaccacac
     6961 cataaacgga ggcaaataat gctgggtaat atgaatgttt ttatggccgt actgggaata
     7021 attttatttt ctggttttct ggccgcgtat ttcagccaca aatgggatga ctaatgaacg
     7081 gagataatcc ctcacctaac cggccccttg ttacagttgt gtacaagggg cctgattttt
     7141 atgacggcga aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt
     7201 gttttgcttt tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag
     7261 catcagtttt ctcaat
//
LOCUS       DAETPZ010000149         7210 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.151, whole genome shotgun sequence.
ACCESSION   DAETPZ010000149 DAETPZ010000000
VERSION     DAETPZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.151"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..332)
                     /locus_tag="K0W59_003821"
     CDS             complement(<1..332)
                     /locus_tag="K0W59_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024658.1"
                     /translation="MAYRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            702..1652
                     /gene="corA"
                     /locus_tag="K0W59_003822"
     CDS             702..1652
                     /gene="corA"
                     /locus_tag="K0W59_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HBI3024659.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1693..2073)
                     /locus_tag="K0W59_003823"
     CDS             complement(1693..2073)
                     /locus_tag="K0W59_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HBI3024660.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2087..2503)
                     /locus_tag="K0W59_003824"
     CDS             complement(2087..2503)
                     /locus_tag="K0W59_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024661.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRRRYIFSFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYLSTFSMLMLAPTDSSKQ"
     gene            complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="K0W59_003825"
     CDS             complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="K0W59_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HBI3024662.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3504..3971)
                     /locus_tag="K0W59_003826"
     CDS             complement(3504..3971)
                     /locus_tag="K0W59_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HBI3024663.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4136..5005
                     /gene="pldA"
                     /locus_tag="K0W59_003827"
     CDS             4136..5005
                     /gene="pldA"
                     /locus_tag="K0W59_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HBI3024664.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5138..6967
                     /gene="recQ"
                     /locus_tag="K0W59_003828"
     CDS             5138..6967
                     /gene="recQ"
                     /locus_tag="K0W59_003828"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBI3024665.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7031..>7210
                     /locus_tag="K0W59_003829"
     CDS             7031..>7210
                     /locus_tag="K0W59_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HBI3024666.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGL"
BASE COUNT         1850 a         1766 c         1806 g         1788 t
ORIGIN      
        1 ttctgctgac cgttttcgat gtacaaaccg agcggcgcat agctttcatc atagatgccg
       61 ccgttcatcg ccatctgcac ctgaccctga ctattaatat ccgccagcag agcatgtaac
      121 gttccccacg cttcgccatt ggctttttgc cagtacattt tcacccgctc tgtttgagga
      181 ttaacggtat acgcctgtac ggtcagcgtc ggatctgaga gtgcgcaatc atcagcggca
      241 acagcaaaca agggaagtaa ggtgagggtg agaaaaatcc gtttgagatt caaggtgatc
      301 attcctttac caatgagtag ccgatacgcc attataggtc ctggatgtgg gattttttta
      361 tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt tcaactattg ttctgtggtg
      421 ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca tgcgcgcacg atcactctaa
      481 gaggacattc gccttggaca cacccagtag atactggctc actatcctgt catccaggat
      541 caactcctaa ggctatccct ttttgctgat agccttagcg gttgtcagcg accttaattt
      601 ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac ccaatttgtt tctgtgtgcc
      661 caccgaactg tccgatattt taagcattgg gagtcccggt catgctgagc gcatttcaac
      721 tggaaaataa ccgactgacc cggctggaag tcgaagagtc acaacccctt gtaaatgcag
      781 tatggattga tcttgtcgaa ccggacgacg acgagcgact gcgcgtacaa tctgaacttg
      841 gccagagcct ggcaacccgc ccggaactgg aagacatcga agcatcggca cgtttctttg
      901 aagacgacga cggcctgcat attcactcct tcttcttctt tgaagatgcg gaagatcacg
      961 ccggtaactc cactgtggca tttaccatcc gtgatggtcg tctgtttact ctgcgtgagc
     1021 gtgaactgcc cgcttttcgt ctgtatcgta tgcgtgcccg tagccagtcg atggtagacg
     1081 gtaacgccta cgagttgctg ctggatctgt tcgaaaccaa aatcgaacag ttggcagatg
     1141 aaattgaaaa tatctatagc gacctggagc agttgagccg ggtgattatg gaagggcatc
     1201 agggcgatga gtacgacgag gcgctctcca cgctggcgga actggaagat atcggctgga
     1261 aagttcgcct gtgtctgatg gatacccagc gcgctctcaa cttcctggtg cgtaaagcgc
     1321 gtttaccggg tgggcaactg gagcaggcgc gtgaaatcct gcgcgatatc gaatccctgc
     1381 tgccgcataa cgaatccctg ttccagaagg tgaacttcct gatgcaggcg gcaatgggtt
     1441 ttatcaacat cgagcagaac cgcatcatca aaattttctc ggtggtatcc gtcgtgttcc
     1501 tgccgccaac gctggttgct tccagctacg ggatgaactt tgagtttatg ccagaactga
     1561 agtggagttt cggctaccct ggcgcgatta tctttatgat cctcgcgggc ctggcaccgt
     1621 atctgtactt taagcggaag aactggttgt aaaaagaagg cggtggctta gcctggctaa
     1681 gccacctgtt attcaaaggc tccaggtatt taaccctttt acctctttct cataaaacca
     1741 tttgttcgtg ttaacagcaa cataggctgc tacggcaatt cccagaatgt taacgccaat
     1801 taatgcacca acaaatagag atagaatgcc aagtaataga actataattg cttttttcca
     1861 taagcccaga acaaataaat agatccagga acagaagaaa gcaatgaaat tcatttgaat
     1921 agttaatcgt tgtcttactt ttaatgcttt aaatgctgct ttatattctg gtgttgctcc
     1981 ccagaatcca ggaaagccat gttgatcata aaaattaaat cggtatttcc atttttcact
     2041 taatgaatca tcgttcatat attccttact cataaatact ccataactat tgttttgatg
     2101 aatcagtagg tgcaagcatt agcatactga aagtggaaag ataacaaatc aaaaaaatca
     2161 tcgaaccatt gcctgaacag gcaaaatctt cggctatcat tgtgatgata gagatgatat
     2221 atactgctaa tgtaccaaaa acataaattt ttatatatat gaaaccacta tcacggagtc
     2281 gttggcaatt catgttgatg acgagataat ggagtgcgat ggtagagact ataacaagaa
     2341 aacctgcttc cccatcgtta aaaaagataa taagaaaggc aaaaatgaaa tttattaaaa
     2401 taaatgaaaa tatatatcgg cgtctggaaa tcttaccgtt agatgttggg ataaacatac
     2461 gtaacataaa ttttacatcc ttgtgtgagt ctcctgtcag catggcaata tgcccactct
     2521 catacaagag tcggcatatt ttttcagaat atatttattt tttatttgga cgttctacgc
     2581 tgcgtataaa tcgcgtccat cacaaaaatt gccagcgcca cccaaataaa ggtgaaagtc
     2641 accatcttat cggcacccgg tttttcacca taaaacgtca cagccagcag gaacatcagc
     2701 gtcgggccaa tgtactgaaa aaagcctaac gttgagagac gcaggcgcgt ggcagcggca
     2761 gtaaaacaca acagcggtac ggtagtgaca ataccggcgg cgatcagcag taaattcagc
     2821 gacatcgggt tttgccccat atggctggtt gaactgtcgg caatagcaaa caggtaaatc
     2881 gccgccacgg gcagcagcca catggtttca attaacatgc cggtttgtgc ttctaccgca
     2941 atcttcttgc gcaccaggcc gtagaaggca aaactaaatg ccagccccag cgcgataata
     3001 ggtagcgaac caaaagtcca cagctggacc aacacgccac atatcgccag aatcaccgcc
     3061 agccattgca tccggcggaa tcgctcgccg aggaaaatca tccccagcac aatgttcacc
     3121 agcgggttaa taaagtaacc aaggctcgct tccagcatat ggtgattgtt caccgcccag
     3181 ataaacagta gccagttgcc accaatcagc acggcagaga ctgccagcat aaaaattttc
     3241 tgtggcgtct gaatcagcgt ttttaaatag gaccactggc ggcaaatgct catcagcacc
     3301 accataaaga aaaacgacca gatcacgcga tgcgtcagga tctcatcggc gggcacgtag
     3361 taaatcagct tgaagtatgc tggcgcaata ccccaaataa aataagcggc aagagcgagt
     3421 aatacgccct gccgcgtttg ttttgcatcc atcgggaata ctcattttta attggtaaca
     3481 gcagtttacc tgcttttatg tcttcaacct accatatagg tggcggtggc actggcaata
     3541 taaagctgtt cttcattgtg taattcaacg cgggcgacgg cgactttatt gcctgcacgc
     3601 aacaggctac tggtagcagt aaaacgctcg cccctgcctg ggcgcagata atcaacgcga
     3661 agatcaatgg tccccatccg cgatagccgc tggcgtagtt catcttcact gatggtttcg
     3721 tggcgggtta aggtacttcc cacgcacacc agaccggcgg cgacatccag cgccgacgca
     3781 atgactccgc cgtgcaaaat gctttgcgcc cagttaccca ccatcatcgg ctgattttta
     3841 aaggccagct gtgcgaactc tttttcgtaa cgctccagtt ccatccccaa tgcgcggtta
     3901 aatggcatgt gataaacaaa catctcaccc actaatttca gggcttgttc agcggtcagt
     3961 acggcagaca tatcatcctt acacttcatt ggttaatgaa atgttgattt tatgcttctt
     4021 tgttgttggt ttctacttta ggaagggata actaacggca atggagttaa gtatgtaaaa
     4081 tagcccgcag aaaaacattc accttatcaa taattcgtta cggagaacac gaccgatgcg
     4141 gactctgcag ggctggttgt tgccggtgtt tatgttgcct atggcagtat atgcacaaga
     4201 ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta tcgccaatat
     4261 gctgcaggag catgacaatc cgttcacgct ctatccttat gacaccaact acctcatcta
     4321 cacccaaacc agcgatctga ataaagaagc gattgccagt tacgactggg cggaaaatgc
     4381 gcgtaaggat gaagtaaagt ttcagttgag cctggcattt ccgctgtggc gtgggatttt
     4441 aggcccaaac tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc aactgtccaa
     4501 tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt tcctcggttt
     4561 tgccaccgat taccgttttg caggttggac gctgcgcgat gtggagatgg ggtataacca
     4621 cgactctaac gggcgttcag acccgacctc ccgcagctgg aaccgccttt atactcgcct
     4681 gatggcagaa aacggtaact ggctggtaga agtgaagccg tggtatgtgg tgggtaatac
     4741 tgacgataac ccggatatca ccaaatatat gggttactac cagcttaaaa tcggctatca
     4801 cctcggtgat gcggtgctca gtgcgaaagg acagtacaac tggaacaccg gctacggcgg
     4861 cgcggagtta ggcttaagtt acccgatcac caaacatgtg cgcctttata ctcaggttta
     4921 cagcggctat ggagaatcgc tcatcgacta taacttcaac cagacccgtg tcggtgtagg
     4981 ggttatgcta aacgacttgt tttgatgaac ggttgagtgg ttggcaaatc tggaatccag
     5041 catccaggat taccctctca gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa
     5101 atagcgcctg tttttatttc aggcaatcgg ggtgaatgtg gcgcaggcgg aagtgttgaa
     5161 tctggagtcc ggagctaaac aggttttaca agaaaccttt ggctaccaac agtttcgccc
     5221 cggccaggaa gaaattatcg acactgtgct ttccggtcgc gattgcctgg ttgttatgcc
     5281 cactggtggc ggaaaatccc tttgctatca aattcctgcc ttattgctaa acggccttac
     5341 cgtggttgtt tcaccgctga tttcgttgat gaaagatcag gtggatcaac tacaggccaa
     5401 cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc gaacagcaac ttgaagtgat
     5461 gacaggctgc cgcaccgggc aaattcgctt actgtatatc gccccggaac gcctgatgct
     5521 ggataacttt cttgagcatc tggcgcactg gaatccggtg ttattagccg tcgatgaagc
     5581 gcactgtatc tcccaatggg gccacgattt ccgcccggaa tatgccgcgc tcggtcagtt
     5641 gcgccagcgg ttcccgacgc tgccgtttat ggcgctgacc gccacagccg acgacaccac
     5701 gcgccaggat atcgtgcgcc tgctggggct gaacgatccg ctgattcaaa tcagcagttt
     5761 tgaccgtccg aatattcgct acatgctgat ggagaaattc aaaccgctcg atcagttgat
     5821 gcgctacgtg caggaacagc gcggtaagtc tggcattatc tactgcaaca gccgggcgaa
     5881 agtagaagac accgctgcgc gcctgcaaag caagggtatt agcgcggcgg cctatcatgc
     5941 cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg atgatctgca
     6001 aattgtggtg gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg ttcgcttcgt
     6061 ggtccacttt gatattccgc gcaatatcga atcctattat caggaaaccg gtcgcgccgg
     6121 gcgtgatggc ctgcctgcgg aagcgatgct gttttacgat ccggctgata tggcgtggct
     6181 gcgccgttgt ctggaagaga agccgcaggg gcagttgcag gatatcgagc gccacaaact
     6241 caatgcgatg ggcgcgtttg ccgaagcgca aacttgccgt cgtctggtat tgctgaacta
     6301 ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg atccgccgaa
     6361 acagtacgac ggttcaaccg atgctcagat tgccctttcc accattggtc gtgtgaatca
     6421 gcggtttggg atgggttatg tggtggaagt gattcgtggt gctaataacc agcgtatccg
     6481 cgactatggt catgacaaac tgaaagtcta tggtatgggc cgtgataaaa gccatgaaca
     6541 ttgggtgagc gtgatccgcc agctgattca tctcggcctg gtgacgcaaa atattgccca
     6601 gcattctgcc ctacaactga cagaggccgc gcgcccggtg ctgcgcggcg aatcctcttt
     6661 gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg
     6721 cggcaactat gatcgcaaac tgttcgccaa attacgcaaa ctgcgtaaat cgattgccga
     6781 tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca accttgattg agatggctga
     6841 acagatgccg atcaccgcca gcgaaatgct cagcgttaac ggcgttggga tgcgcaagct
     6901 ggaacgcttt ggtaaaccgt ttatggcgct gattcgtgcg catgtcgatg gcgacgacga
     6961 agagtagtca gcagcataaa aaagtgccag tatgaagact ccgtaaacgt ttcccccgcg
     7021 agtcaaatgt atgttgatgt tatttctcac cgtcgccatg gtgcacattg tggcgcttat
     7081 gagccccggt cccgatttct tttttgtctc tcagaccgct gtcagtcgtt cccgtaaaga
     7141 agcgatgatg ggcgtgctgg gcattacctg cggcgtaatg gtttgggctg ggattgcgtt
     7201 gcttggcctg
//
LOCUS       DAETPZ010000150         7186 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.152, whole genome shotgun sequence.
ACCESSION   DAETPZ010000150 DAETPZ010000000
VERSION     DAETPZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.152"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            27..878
                     /gene="hchA"
                     /locus_tag="K0W59_003830"
     CDS             27..878
                     /gene="hchA"
                     /locus_tag="K0W59_003830"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HBI3024667.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(986..2344)
                     /gene="hprS"
                     /locus_tag="K0W59_003831"
     CDS             complement(986..2344)
                     /gene="hprS"
                     /locus_tag="K0W59_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HBI3024668.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLRELKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLESLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2344..3015)
                     /gene="hprR"
                     /locus_tag="K0W59_003832"
     CDS             complement(2344..3015)
                     /gene="hprR"
                     /locus_tag="K0W59_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HBI3024669.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3148..3561
                     /gene="hiuH"
                     /locus_tag="K0W59_003833"
     CDS             3148..3561
                     /gene="hiuH"
                     /locus_tag="K0W59_003833"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HBI3024670.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3670..4674
                     /gene="msrP"
                     /locus_tag="K0W59_003834"
                     /pseudo
     CDS             3670..4674
                     /gene="msrP"
                     /locus_tag="K0W59_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4675..5309
                     /gene="msrQ"
                     /locus_tag="K0W59_003835"
                     /pseudo
     CDS             4675..5309
                     /gene="msrQ"
                     /locus_tag="K0W59_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
     gene            5567..6217
                     /gene="zinT"
                     /locus_tag="K0W59_003836"
     CDS             5567..6217
                     /gene="zinT"
                     /locus_tag="K0W59_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HBI3024671.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         2034 a         1626 c         1509 g         2017 t
ORIGIN      
        1 aactaagcaa caataaggaa tacactatga ctgttcaaac aagtaaaaac ccgcaggtcg
       61 atattgctga agataatgcg ttcttccctt cagaatattc gcttagccaa tatacgagtc
      121 ccgtctctga tcttgatggc gtggactatc caaaaccgta tcgcggtaaa cataaaattc
      181 tggtgatcgc cgcggacgaa cgttatttgc cgaccgataa cggaaaactg ttctcgacag
      241 gtaaccatcc gattgaaacg ttgctgccgt tgtatcatct ccatgctgca ggtttcgaat
      301 tcgaagtggc gaccatttcc ggtctgatga ccaagtttga atactgggct atgccgcaca
      361 aagatgaaaa agtaatgcca ttctttgaac agcataaatc gttattccgt aatccgaaga
      421 aactcgcgga tgttgtcgcc agcctcaacg ctgatagcga atatgcagca atctttgttc
      481 ctggtgggca tggcgcactt attggtttac cagaaagcca ggacgtggct gccgctttac
      541 agtgggcaat caaaaatgac cgttttgtta tctccctttg ccacggtccg gcggcttttc
      601 tggcacttcg ccacagcgat aacccactga atggttattc tatttgcgca ttcccagacg
      661 ccgcagacaa acaaacgcca gatattggct atatgccggg tcatctcacc tggtacttcg
      721 gcgaagagtt gaagaaaatg ggcatgaaca tcattaatga cgacatcacc gggcgagtac
      781 ataaggaccg taaacgtctc accggcgaca gtccttttgc agcgaatgcg ttgggtaaac
      841 tggcggcgca ggaaatgctg gcagcttacg cgagttaatc cctgttacat tgtgatgtaa
      901 gcgcataatc aatcgcagcc acgttaatac tcacgacgtg gctgcgtttt ttcacggtta
      961 acttatggcg tactgaagcc cgatgttaat ttctttgcgg taacgtaatt cggaacacgt
     1021 tatgcttact gagatagtga tacgtagcac ttcctccatg taattcggca atcgctttga
     1081 ctaaagaaag gccaagtccc tgacctacgg aatgacgcga attatctccc cgccaaaatc
     1141 tacggaagag tttttcaggc tcattaattt tcgttccagg actggcgata tcaatattaa
     1201 gagagccgtt ggcatcaaga aaactggtta tatgaatacg cgatttttct ggtgaatatc
     1261 gaatggcgtt aacaataaga ttcgataaca ttcgttgcag taaaatttta tccgcaaaga
     1321 tttgttgatt gcacttgacc ttaaagcaaa tctctttctc gtctgaaaga gattcaagat
     1381 agtccaaaag attttcaact tccttattga gagaaagcga atccagtttc accaaaacat
     1441 tgtttttgtc tgccctggca agaaacagta tattctctgt taaccgcgaa atattttcca
     1501 gctcttcaat gtttccggca attgtttttt gatattcagc gatacttctg atttgactga
     1561 gcgtaacctg attctgaccc agtaatgcat taattggcgt tctaagttca tgagcgagat
     1621 cgtcagcaaa ttgactaagg cgctcaaaat ctttgactaa agcatgatgc attttattca
     1681 acgcctgccc aagaggtttt agttctctcg gtaatgcgtt aacctcaacc ggcactcggc
     1741 tatcgttata attcagcgct tccgttacgc cactcaactt tttgagctct cgcaaccccg
     1801 tcctgattaa caacggactt aatactgagc acagtataat ggcgacaatg caaattataa
     1861 tgctattaat tttatactgt tcaagcatat tatgtctggc tgaagccaat ttagccacag
     1921 taaccgttaa tgatggcgaa acttcatcaa tgttaattcg taaagcatct atctctgtat
     1981 cattaataat gcttctgtaa atgcctgctg cgctgattgt ctcactagca ggtatgttat
     2041 ttaacatgtc atcactgaca tttgtccggt taacaatttt attgatgcca tcactatgaa
     2101 tgatcaaaat atcctgacta acatccatca tccggttaaa gtacacaggt aacgtatctg
     2161 gatttacccc atcaattaac aactgcttga tctgcgctgt ccggttaatg agtgttgtat
     2221 catcgcgcca tttcaactca cttgccaggc cattatagag agtccataca attccagcgc
     2281 cagcaacaga ctgcagaaat ataaaaagca aggttaaacg gacggttata gataatcttt
     2341 tcattatttt tttaccgcta cgaatgaata gcccatcccc cggattgtgg cgattagctt
     2401 ttcaggaaaa ggatcatcaa cttttgcgcg gagcctgcga atggcgacat ccaccgtatt
     2461 ggtatcacta tcaaagttga ttccccaaat ttcactcgca ataaccgttc tgggtataat
     2521 ctcgccagct ctggaggcca gtagccaaag taactgaaac tccttgcgcg tcagtgtaat
     2581 actgatattg tccctgctga cactttgact gacagagtcc atttttaagc cactgatttc
     2641 taatgttgaa ttcagagcgt gatgttgcct taattgtgcc cgaacccttg ctaacaactc
     2701 agaaaatgaa aaaggtttta ccagataatc atttgcccca ctgtccaacc ctctgactct
     2761 gtcatcgaca gaatcccttg cagtaaggca aataacaggg gtttgctttg ccgttcttaa
     2821 cgtttgtaag atctgccagc catccatacc cggaagcata atatccagaa tgatcaatgc
     2881 ataatcatcc ttcagcgcaa gataaagccc atctctgcca tcagaaacgg catcaatgac
     2941 ataacccgct tcggaaagcc cctgcgttac ccattcctgg gtcctttgat tatcttcaat
     3001 aagtagaatc ttcatgaaaa taatatgcca taaatcatat gttatagaaa caacctggtt
     3061 cattacaaaa ttgtaatgct gctgtaaggt taccctggac gttttttcgc tatcctcaaa
     3121 actcattcac atgacaagga tataaacatg ttaaagcgtt atttagtact ctcggtagca
     3181 acggcagcat tttcattacc ttctttggtt tatgccgcac aacaaaacat tcttagcgtg
     3241 cacattttga accagcaaac aggaaaaccc gctgccgacg tgacagtcac tcttgaaaag
     3301 aaagcggata acggctggtt acaacttaat accgccaaaa cagataagga tggacgaatt
     3361 aaggcactgt ggcccgagca aactgcgact actggcgatt accgtgtcgt atttaaaacc
     3421 ggggactatt tcaagaaaca aaatcttgaa agtttcttcc ctgagattcc cgttgaattt
     3481 catattaata aagtgaacga gcattatcat gtgcctttgt tactcagcca atatgggtat
     3541 tcaacctatc gtggcagtta atgcgtaaat atgtaaaagc tgatctctgc tactgttagg
     3601 gaggtgactc cctgacagat taacagtaaa cagctcttgt tggttaacga caaaaaagtg
     3661 tgatggctta tgaaaaagaa tcaattttta aaagaatcag atgttacagc cgagtcggta
     3721 ttctttatga agcgtcggca ggtgttaaaa gcactgggca tcagcgcagc tgcattttct
     3781 ttgcctcacg ctgcgcatgc cgatctgctt agctggttta aagggaacga tcgtccgccc
     3841 gcccccgccg gaaaaccgct ggagttcagc aagcctgccg cctagcaaaa taacctgcca
     3901 ctgacgccag cagataaagt ctccggttat aacaacttct atgaattcgg gctggataaa
     3961 gacgatcccg cggctaatgc tggtagcctg aaaaccgatc catggacact gaaaatcagc
     4021 ggcgaagtgg caaaaccatt gaccctcgat catgatgatt taacccgtcg cttcccgctg
     4081 gaagagcgta tttatcgtat gcgctgcgtg gaagcgtggt cgatggtggt gccgtggatt
     4141 ggttttccgc tacacaaatt gctggcgctt gccgaaccca ccagcaatgc gaagtatgtc
     4201 gctttcgaaa cgatttatgc gccggaacag atgcctggcc agcaggaccg ctttatcggc
     4261 ggcgggctga aatatcctta tgtcgaagga ttgcgtctcg acgaagcaat gcatccgctc
     4321 acactgatga ccgtaggtgt ttatggcaag gcgttaccac cgcaaaatgg cgcgccggtg
     4381 cgactgattg tgccgtggaa atatggcttt aaagggatta aatcgatcgt cagtattaag
     4441 ctgacccgcg agcgtccgcc aaccacctgg aatctggcag cgcctgacga atacggtttt
     4501 tacgccaacg ttaatccgca tgttgatcac ccgcgctggt cacaggctac cgaacgattt
     4561 attggttcag gcggcattct cgatgttcag cgccagccaa cactactgtt taatggttac
     4621 gccgaccagg tggcatcgct gtatcgtggc ctggatttgc gggagaattt ctaaatgcgt
     4681 ctgacagcaa aacaggtgat atggctgaaa gtttgcctgc atcttgccgg attgttgccg
     4741 tttctctggc tggtctgggc gatcaatcac ggtggactgg gtgccgatcc ggtgaaagat
     4801 attcagcatt ttaccggtcg cactgcactg aaatttttgc ttgcggcctt gttaatcaca
     4861 cctctggcac gctacgcaaa acagccgtta ttgatacgca ctcgccgcct gttaggctta
     4921 tggtgctttg cctgggcgac actgcattta accagttacg cattgctgga gttgggcgtg
     4981 aataatctgg cgttattagg taaggagtta attacccggc cttatttaac gttaggaatt
     5041 atcagctggg ttattttgct tgctttagcg ttcacttcaa cccagtcgat gcagcgaaaa
     5101 ctcggcaaac actggcaaca gttgcataac ttcgtctatc ttgtcgcgat cctggcccca
     5161 atacattatc tgtggtcagt gaagattatt tcaccgcagc cactcatcta cgctgggctg
     5221 gctgtactgc tttagcctta cggtataaga agttgctttc ccttttcaac cggttacgca
     5281 agcaggccca caacaaatta tctctataac ttcatattgc cgacaaactt cgcgttatcc
     5341 cggccggatg cagcgcgagc gccctatccg gcccacacag atcgcatcaa gttatgtttc
     5401 gtttgtcatc aacctttcat caccagaata atttatttta tttcaataag atagttcaat
     5461 acacctgaga ataccatgct ctcatttcct aatgagttgt tgcatttttt catatgttac
     5521 aatataacat tacacataat gtacattaac tctggaggaa attgttttgg cgattcgtct
     5581 tcataaactg gctgttgctt taggtgtctt tattgttagc gctcctgcct tttcgcatgg
     5641 tcatcattct catggcaaac ccttaacaga ggtcgaacaa aaagctgcta atggtgtttt
     5701 tgatgatacc aatgtacaaa accgaacgct cagtgactgg gatggagtct ggcaatccgt
     5761 ttacccttta ctgcaaagtg gcaaacttga tcccgtcttt cagaagaaag cggatgcaga
     5821 taaaaccaaa acatttgctg aaattaaaga ttattatcgc aaaggttatc cgacagatat
     5881 cgaaatgatt ggcattgagg acggcattgt cgaattccat agaaataatg aaacaacatc
     5941 ctgtaaatat gattacgatg gatacaaaat actcacctat aaatcaggca agaaaggcgt
     6001 tcgttactta ttcgaatgta aagatcctga aagcaaagcc cctaaatata tacaatttag
     6061 cgatcatata attgcaccac gaaaatcatc tcattttcac atttttatgg gtaatgattc
     6121 ccagcaatct ttattgaatg aaatggaaaa ctggccaacg tattatccct atcagttgag
     6181 tagcgaagaa gtggtcgagg agatgatgtc tcattgattt ttttgcgctg acttcagtta
     6241 tttaacagaa cagtgcctgg tagtaacaac aataatttac caagacaaat aagtaagaca
     6301 aactcagaca cgctcatact tgagtcgtgt cttttttatt atgtctgtag aaaaaaactg
     6361 agaaaaaggc gaacaataaa tacacaactc agccatacag actggcagcg caaatgtttc
     6421 caggagcaag tcatctgtca tggtaaagct aaccagaaat ataggttaat tttggtgttt
     6481 aaaccttttt tacttgttag tatgatacaa aggctttcaa aaaagctggg aaattcaaca
     6541 actcttggaa gttatggact ttgtcccgcg gctttaatct gacggccgcg ttcctttttt
     6601 ggctcaatta ttgacccctt ctctacgcaa cttcagttcc cgccagcaac tttgcggcag
     6661 tttcatagga tcaatgtcta aaagaataat ggtgaccgat aagaaaacga ctgaataact
     6721 gtagattttc gctcgaaacc ttcctgtcag ctccatagcg aatcaagcgc tgaatgtcac
     6781 agtatcgaac agaaaacagt gacgatctaa cccttcaaga atattctacg attgttctgt
     6841 ttaggaaaag caaggcggga agtcgggaga taagtcattg ataaagtggc ggagagaggg
     6901 ggatttgaac ccccggtaga gttgccccta ctccggtttt cgagaccgat gatacagagt
     6961 cgaaaaatca aagagttaaa aatttttctt ggaaaaaatg tcttaaaatc gtacacgaaa
     7021 aatcaatgaa ttacataaca ttaaagaaga atattccaag ccttcttgag tccaccacct
     7081 gttttcaggt gtcattcaga cgtgatttgc cccagattgg gaaagcacgc ttttttctca
     7141 gcttattccg ttttgcctgt gtgttgaata attccactgc agttat
//
LOCUS       DAETPZ010000151         7045 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.153, whole genome shotgun sequence.
ACCESSION   DAETPZ010000151 DAETPZ010000000
VERSION     DAETPZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.153"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..730
                     /locus_tag="K0W59_003837"
     CDS             <1..730
                     /locus_tag="K0W59_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBI3024672.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIAWAITTQQKEYQA"
     gene            complement(1033..1803)
                     /locus_tag="K0W59_003838"
     CDS             complement(1033..1803)
                     /locus_tag="K0W59_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024673.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPLLPADGTQVSF
                     PYACEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1848..2282)
                     /locus_tag="K0W59_003839"
                     /pseudo
     CDS             complement(1848..2282)
                     /locus_tag="K0W59_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2294..2515)
                     /locus_tag="K0W59_003840"
     CDS             complement(2294..2515)
                     /locus_tag="K0W59_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024674.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2516..3199)
                     /locus_tag="K0W59_003841"
     CDS             complement(2516..3199)
                     /locus_tag="K0W59_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBI3024675.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3263..3574)
                     /locus_tag="K0W59_003842"
     CDS             complement(3263..3574)
                     /locus_tag="K0W59_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024676.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(3571..4473)
                     /locus_tag="K0W59_003843"
     CDS             complement(3571..4473)
                     /locus_tag="K0W59_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HBI3024677.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4913..5872
                     /locus_tag="K0W59_003844"
     CDS             4913..5872
                     /locus_tag="K0W59_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HBI3024678.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5872..6267
                     /locus_tag="K0W59_003845"
     CDS             5872..6267
                     /locus_tag="K0W59_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HBI3024679.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6450..6830
                     /locus_tag="K0W59_003846"
     CDS             6450..6830
                     /locus_tag="K0W59_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBI3024680.1"
                     /translation="MTMATINARIDDDINNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
     gene            6876..>7045
                     /locus_tag="K0W59_003847"
     CDS             6876..>7045
                     /locus_tag="K0W59_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3024681.1"
                     /translation="MARYDLPDEAWTIIKPLLPPEPATPRAGRPWAEHRKIINGMFWV
                     LCSGAPWRDLPER"
BASE COUNT         1913 a         1826 c         1716 g         1590 t
ORIGIN      
        1 tgatgcagaa gccatatgtg aagcggcttc acgaccatca atgcgttttg tccagccaag
       61 aactgagacc cagcaagcaa taagggcatt acaccgggtc agagaatcgc tcatcagaga
      121 taaggtgaaa acaaccaacc agatccacgg tttcctgctg gaatttggaa tcagcctgcc
      181 gacaggggat gccgtaataa aacggttgtc tctcgtattg gctgaacacg aaatccccga
      241 atatctgagc cgccttttgg tgaggttgca tacccactac ctttaccttg ttgagcaaat
      301 agcagagctg gaatcagaat taagtcagtc aatcaacgct gatgataccg ctcagcgcat
      361 tatgacaatt cctggtgttg ggccgattac tgccagctta ctatcgtcgc aacttggtga
      421 cgggaaacag ttttcatgca gcagagattt tgcggcttcc acagggctcg ttccccggca
      481 gtatagcact ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg ataaaaattt
      541 acgccggtta ctggttcagt gtgcccgctc attcatgatg cagcttgaac gccagcatgg
      601 aaaacttgct gagtgggttc gggagcaact caacaagaag cattcgaatg tggttgcctg
      661 cgctctggca aataaactgg cccgaattgc ctgggcaatc actactcagc aaaaagaata
      721 tcaggcctga tatatagatg ctgacaaaac tcaattagct tattgcaaac aataagatac
      781 aagttaccgt ctggttttgc gaagattgat tattgatgac atgaacggca aatcgacctg
      841 attaaaaacc taacagggga aaatggctca ctgaagccga ggggattttt aggattatca
      901 ggtgcggaac tcatcgaggc gcgggcatcc cttgctcaca gagacgccgg atagatttaa
      961 gcaagcccac cattaatcaa aaatcagtgt tgcaaaaacg ggggtgacca tagattttcc
     1021 ccgccatcgt catcatttcc ggatttttgc cagggcatcc gccatgctgc ggatttcggc
     1081 ccgggcttcc cggacaactt cccgcagaac accacggtca aaccaccggc ggggcggttc
     1141 atccggtata cgcagcacat cgcaggccag cccactggac aggatatgct caatacaatc
     1201 gctgaccaga tacatttcca ccgccgaaaa acgtgcgccc cggttcagga tggcatcgag
     1261 caccacgggc aggttttcat cgtcttcatc gagccagcag gggtgatcgc tttccttcac
     1321 gaccaggcga aagcggcgcg tctggcgggt cagcaacgtc tgaccgcttg tggtcagcgc
     1381 cgcccggata cgccgcgcat cttctgcccc ccggcaactg acactgcata ctgcatcgcg
     1441 caccgcatca agaactgccc tgatttcgtc ttcggtcagc cattcacagg catacgggaa
     1501 agacacctgc gttccgtccg ccggaagtag cggcagcgac tgccactgac tgccggacag
     1561 aacaagttcc gctccggttc cggtcagttt ctgaatggcg gaggccaggt gcggcggcag
     1621 cacgccatcg tggcggttaa cgagtaacgt cgccagccgg ggcgcatcca gccgcacgta
     1681 aatgctgatc cagcccggat agttcgggtt acattggtca acatccgtct cttccggcca
     1741 gccctgcgcc agcagcaggt tacgggcgtg acgacaaatc agagcattca gcgtttcatt
     1801 cataattcat gaactccctg tatcgccgcc cgcaggcggc gataacatca gtcgttttcg
     1861 tgtgtgttgt ttttcagcag gctgcgggcc tgatcaacca tcgcctgtcg gcgggtaacg
     1921 tattccggga gggggaaacc gtgatcccgg gcagcccatt cacgtttcca cagtgcctgt
     1981 tcagcgatct gagtcagggt ctccagcagt tcagcaggga cggtcacctg gcggttttcg
     2041 cgcacgttgc tgataatctc catcgccgcg gcggaagaaa taccggactc aatccgctag
     2101 tcttccggga tgtcctccag gtgcgccagg acggtcctga tttcatgatc ggacagggaa
     2161 atatccattc cgtcagcgag ggcctgaatc tcttccgggg accagataac cagcatcagc
     2221 ggcgtgtctc ccgaatactt tgctgccagc gcccggcata gcgtttcgca tgttccgtac
     2281 atactctctc ccctcaatct ttgaagcagg aatccagttc cagaatttcg gtatatgcct
     2341 ccatcaggag gcggtttgcg cgaaacaacg tctgacgcac cagacgttcc ggcagggtgt
     2401 cagcattaaa gctgtaccgc cgctccatct cattcatggt tgtacgcagg tcgcacatgg
     2461 cattggccac ctccgccact tcggcgcgaa gtttttcgtg tccggcatag ttcatttacg
     2521 cagcctccgg cataaaccag tcatcattcg cggccccctg ctgcatggcc cgctgtacgg
     2581 cagccagtcg ttgctgtccg gcacggtgat actgctcaag cagctcgata ccgatatacc
     2641 ggcgtccgga ctggagggcg gcgacgcagg ttgaaccgct gcctgcaaac gggtccagca
     2701 ctatcgcatt cgggtgtgtg aagctctcaa tcagcggttg caggctggta acgggctttt
     2761 ccgtcgggtg atggcggttg cccgaatatt tccagcccaa cacgtccggc agcgggtttt
     2821 gtggcagacg tggacggcct tttgccagga tgtaggcgca ttcgtggcga tagcccacat
     2881 atgcggcctt cgatgtgtag tttttggtga agaccaggtg accaacaacg ctgaatcccg
     2941 catttttcca ggcagacata aagcgatcga cgcggttcca gccatagaag ctcaccatca
     3001 gcgcgtcttt tttgagtacg cggtacattt cattacaggc cggttgcagc cattcatcag
     3061 ttttatcacc agcaatggta cgcccctgac ggtcacgaaa accgacgaga tatggcggat
     3121 cggtgaggat gaaatcaatg gcgttatcag gaatacgggc cataacatcg atgcagttac
     3181 cgaggacaaa acgggacata acagatcctt ctcttacaga gggcacccct gtggtgcccg
     3241 ttccccgctg cctgtgggct gttcatcgcg tccggtaacg ggtcaggccg accgtggaat
     3301 accgcagccg tcgtcttttt gacagcaagg ttatgccgcg aaagcggctt gaagggcgtt
     3361 catcagaaaa gggcgacggg ctggcgtaca ctgtcaccga cgccagccgg gagcggtaaa
     3421 agcgcccgtc ccgtgcccgc cggacgcggg acactgacca caggcagcgt ttatgccgcg
     3481 ccgcgtcagc cggcgcagta aaaagaggag cacggcgaca tcctttgtcg gaaaagaaac
     3541 gggcggttgc ccgcccgtct ccgtcatact tcatccgcca taatgcgcca cattgtcgac
     3601 tatccacgca ggtccggtga cttccggctg ctcatcgtca tctgtcgggg aagaccattc
     3661 aagttccccc cacagcacat cgacgagctc tccacgctca tacaactgcc agccacagaa
     3721 atcacagccc tgttcggcat accagtgctc cagcgtgcag ctgaaacggc ggcttaattc
     3781 tgcaataacg tcgctttccg gctgacacca cggcgtgtcg aaatccacct gaatgaagtt
     3841 ttctccctga ctgctgatat tcaggtcata tccacaaggc cacttcacgc cataccgttc
     3901 cgtataccag tgatatgcag aaggaacacc gttcagaaca ccaccattaa agccgttcac
     3961 ttcaacatcc aggcgggtcg gcagaaccgt cagcaggtca aacggcatgg ttttttccgg
     4021 cactacgttg tcacacagac ggttccacca cacagataca tcctcgttac cccatatgtc
     4081 acaccagtca cctctttttg cggtgaaatg cacagtgatg gtttccctgg catcatccgg
     4141 taatccttcc cagcgacgct ggccagtacc gctctgtaac cagagttcat gcagcatcag
     4201 gcagttttgt tcatccagca acacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc
     4261 gatattttcc gtcgatacag caccacgtcc ggcagccgtc actccggggc actgttcaga
     4321 gcggatattt tcagtgatct gcagaagtcc ggcacttccg gccagaaaca gctgaatacc
     4381 ttcatttgtg gcgcggcgat aaagcggtgt gactgcaccg ctggccagtc gtttaatctc
     4441 agcgatctgt gccggttcac caggaaaata catacgattc gagcaccagt ttggcatggg
     4501 aattctcctc tcacatgcgg cctccatcag gccgtgccgc acctgtaacc gggcgaccgc
     4561 atacgaatgc aggtgcggcc ccgaagccgc agcgcgtgga ggaggaatga cgaaacgtga
     4621 ccgggtgagg aggccggcga cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc
     4681 cggcacggcc tggttaagta gtatccgtct cagttcgtgg cgcaaaatga gaaaccaata
     4741 agaactcaaa atgaaccaca acaagaacat aaagagactc taaaagagca acaccgcttg
     4801 acatagactc aaaatgagtc acaataagaa acaataaaaa actcaaaaag atgcaccgat
     4861 ataaaggatg ctcaatttga gataaacaca aactcaaaac gaggcaaaaa gaatgctaaa
     4921 agtatcatgc gatgatggtt caactaacgt aaaactggcc tggcttgaag atggcgaggt
     4981 aagaaccagt ctgtcaggaa acagctttaa agaaggctgg aacccgggac tgtttaatgc
     5041 cggcaaagtt tacaattacg tcgtggatga aaaaaaatac acctatgatt tgggaagcac
     5101 tgctgtcata ggtacaacac atgtcagtta tcaatacagc accaccaatc tgcttgccat
     5161 acatcatgca ctgctgacat ccggccttca gccacaggat gtagaactga cagtaacatt
     5221 acctgtgacc gaattctttg ataatgacaa tcaaccaaat gaagaaagaa tagaaagaaa
     5281 aaaggcaaat gttctccgtg agatatctct gaataaaggg gaaacattta aaattaaaaa
     5341 agttaatgta atgcctgaat ctttgccagc tgcatttgaa tcactaaaaa aagataaagt
     5401 aaacaaactt gaacgcagcc tgattatcga tcttggagga accactctgg attgtggttt
     5461 aattctcggt gcatttgaag gcatttctga aatccgtggc tattcagaaa tcgggacatc
     5521 ccgcatcact cataccgtca tgaatgcgct gacgaaagcc tccacaccat gtaattattt
     5581 tattgctgat gagttaataa aaaacagaca cgataatgaa tatctccaga cattaataaa
     5641 tgatgtagct gaaataaaaa acatttctca tgtaatagac agagaagtga aatctctggc
     5701 agaaagcatc aggcaggaaa tttcaacatt ctcaggaatg aaccgaatct atctcactgg
     5761 cggaggagcc gagcttattt accctcacat aaagcaatat ttcccgaatc tgaaagtgaa
     5821 caaggtggat gaacctcagt ttgcattggt gaaagcaatg gtgcatgcgt aatggaaagc
     5881 agtgatccaa aaaaaagaaa gaaagttgtg gcatacctgc acccggcact ttatccgcag
     5941 gacaacctga cacaacaaac aatagactct ctcccagtac aaatgcgggg agatttctac
     6001 agacaatccc ttatttgcgg agcagctctt tattctgttg acccccgact gttaacgcta
     6061 atcagcggtt ttttcagtga gaaaatcact gcagaaaatc tggtgaaact tatagaacaa
     6121 acaacaggat acacatccac atcaattgat ataagtgtgt taaaaaacat catagaggca
     6181 tcttcagaaa acaaatcaga gagcatcact tcaaaagatg atttcgaaga acaaaccaga
     6241 cgcaatcttt ctatgttaaa aaaataataa aagaagagca ggttcagtca attacctgct
     6301 cttcttttat acatactaaa aaatcagatt tttacgaata ttcagattat cataaaaaat
     6361 gcaacagaca ggaatgcaat aattttgcat tatctcgttg cttgtaatgc aaatatgcat
     6421 tattgtcagt gtgtgacaat aacagagaga tgacgatggc gaccattaac gcacgtattg
     6481 atgatgatat aaataaccaa gcagatgaag tgctgaagct gatgaatatc agtcaaactc
     6541 aggctattgc agctttttat cagtatatta ctgaacaaaa gaaactgcca tttgtgatca
     6601 cctcaattgt aaaaacgccg catgatttat tacgtgaaag tacagacatg ttagctgaag
     6661 ctcttgcagt gatttccaat ctacaggtgt ggactgaaca gcaggatggg attggaaaag
     6721 caaagctaat ggagtattac cgccgcctcg acgcactgta ctgttgtgca aaagagaaaa
     6781 taggtcttct ttcagataac cgggatgctg aactaggctg tgtcccttaa tcatctgagt
     6841 tatagtaact gtctgtttac acaacatatt gaactatggc tcgctacgac cttcccgatg
     6901 aggcatggac catcatcaag cctctgttac ctcctgaacc cgccacacca cgggccggac
     6961 gcccatgggc tgagcaccgt aaaatcatca atggcatgtt ctgggtgtta tgttccggtg
     7021 caccatggcg cgatttaccc gaacg
//
LOCUS       DAETPZ010000152         6857 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.154, whole genome shotgun sequence.
ACCESSION   DAETPZ010000152 DAETPZ010000000
VERSION     DAETPZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.154"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..171)
                     /locus_tag="K0W59_003848"
     CDS             complement(<1..171)
                     /locus_tag="K0W59_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3024682.1"
                     /translation="MSRKNQRYSTEFKAEAVKTVPENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLNTPGS"
     gene            complement(861..2570)
                     /locus_tag="K0W59_003849"
     CDS             complement(861..2570)
                     /locus_tag="K0W59_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HBI3024683.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVANSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(3002..3721)
                     /locus_tag="K0W59_003850"
     CDS             complement(3002..3721)
                     /locus_tag="K0W59_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HBI3024684.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3846..4402)
                     /locus_tag="K0W59_003851"
                     /pseudo
     CDS             complement(3846..4402)
                     /locus_tag="K0W59_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4386..4644
                     /locus_tag="K0W59_003852"
                     /pseudo
     CDS             4386..4644
                     /locus_tag="K0W59_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(4753..5331)
                     /locus_tag="K0W59_003853"
     CDS             complement(4753..5331)
                     /locus_tag="K0W59_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024685.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(5752..5989)
                     /locus_tag="K0W59_003854"
                     /pseudo
     CDS             complement(5752..5989)
                     /locus_tag="K0W59_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(6176..6605)
                     /locus_tag="K0W59_003855"
                     /pseudo
     CDS             complement(6176..6605)
                     /locus_tag="K0W59_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6653..>6857)
                     /locus_tag="K0W59_003856"
     CDS             complement(6653..>6857)
                     /locus_tag="K0W59_003856"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024686.1"
                     /translation="LNIPFFVDARNKKLVFWFVRVDDEGYPEIARCMEREFATIPAGI
                     NADGMYCPECGTVHWPDGVIPPF"
BASE COUNT         1867 a         1573 c         1269 g         2148 t
ORIGIN      
        1 ggagcctggt gtattgagcc cttttcttgc ggcggtaacc cattgtccta aagtgccttc
       61 aggaagagat aatcgggaag cgccttcact gatcgaaagc tgattttcag gaaccgtttt
      121 gacggcttcg gctttaaact ccgtagagta acgttggttt tttctgctca ttattggctc
      181 cttctgatgc cattctattt caggaaggag tgtccgttaa actcaggcta cctcagataa
      241 gcttgataca actggtctgg tatatacagt ttctttgagt gcggttacgt ccacagaggt
      301 aacggtaaaa gtaacccctt gtcgggacca ctccgaccgc gcacagaggc attttcatcg
      361 ctgacaggcc gcattgcctc gcagggctgg aggaggatcg gtgatatccg gacgaccgta
      421 ccggcttacg ccgggcttcg gcggttttac tcctctgcca tgtgcaacaa cggagtgccg
      481 ccttacatgc cgctggcgcg gcatatcctg gtaacatttt tgttgatacc accttccggc
      541 cttatcctga ccagtgggct ttcctggcat cgatgatacc gatgaacgcg catgatattg
      601 agccgacgat attacgggca actggaaata ctcactctct ggatgtgacc tttatccatg
      661 aagaagattt ggccacacca tggaaacctg aacaatcatc agtttacgcc catgtaaacc
      721 cctacgggat atttgaactc gatatggaaa caaggttgcc aatcgaggtg gttgcctgaa
      781 agttggcagt tacccggtca ctacaccttg ttggagtgaa catatcaaaa acaggtaatt
      841 acacaattta aattacagtc tcataaaaaa tgattaaatc tatttattat ttcaataaac
      901 gtttttttca tcttctttga aaattgttct gctatagtat ccggttccag attgttctct
      961 tttattattc tacaatactc taatattgtt tctttgtctc tatttactac agatacaaat
     1021 acaccaggaa taccatcttt attagttgcg gacagcattt cgaccaatcg tcttgtggta
     1081 aaactatagt tttttagaat attacaaaag gcacgtattg tttcggtatg gccattataa
     1141 aatgccataa aaagtcctga tgtaccgttg ggatttttta tccataataa ttcatctata
     1201 tattcctggt gaggggggag tatttttagt atatctccat acgccataat ggcctttgtg
     1261 tgcccatttt gaagtgccat aaacaaccca tgctttactt tcaataattc tttaatttgt
     1321 tcactactaa gcattttctt attaagcaga cttccgaaca tacgaatact atctgcatgt
     1381 ccattctgca atgcaaaaaa taacccaggt aaatcatggc tgtctttcgc agctaataga
     1441 tataatctct tatcggaagt taacggggtt ttaaagacta tttccccata ggcttttatt
     1501 gtgtctacat gtccgttcat aaaagacatg cataaacccg gcacattgtt tgcccctacc
     1561 gcagcaagca atgtaagtat tgtttcttga ctaagattgc acatatttaa tatcttacca
     1621 tatacttgaa taatgtctga atatccgttt tgaagtgcta tataaaatgc aggagtacca
     1681 tcagagcttc tcgtggccag aaggttcacc agtttagatt gagaaatttc accatttttg
     1741 tatttttcca tcagtataaa taatacttct gtcagattct ttgttatcgc atgatatata
     1801 actctggaat tgatcaattc atctggtaaa agtttcactg tgtttgttag aggggaatta
     1861 tgcctatctt caaatattac ctgcccgggt tgctccagac cataaatttt ccaataaaaa
     1921 tcatcaaaaa aatccttagc ggataattgg ctcagttcga aatggctatc acaagtaaat
     1981 acagtacgtt tatgagtaac agttcgatta ggatcgaaaa actgaacaac ccatttttca
     2041 ctttcttgta cgcgctttat tttcaaccct aacgccattt ggtggtttaa tgtgcatgct
     2101 gataatgttc tgacaaagtc tccatttttt ttcatgttat aaaaaaaatt agtaataact
     2161 gttccccact ttttattttc cacaaaataa atataagctg gtgattttga aaaatcttcc
     2221 gttttttcca aacatacatt ttttgcaata gcatcagggg tgctaagaag ctgatagttt
     2281 accttcccat ttgtttccat aaaattcatt tcccaatagg aagctaggtg acggcaaact
     2341 atttgtcttc catatatatc ttttgccact ccatttatat ttaccttcac atttctcttt
     2401 tctgaaaaat aaggtgctgt tttattctta aactgaatta gttcactgga gtttgcgacc
     2461 ctcataatag aacttgttat tgttctatta tctctgttaa cagacatatc tgttgataac
     2521 gaattttttg ttgaaaatat actagatgaa aatgttttat ttaacggcat agaagacaac
     2581 catagagatg ttataatact attagattgc agttactaat agtaaaagcc tccttacaag
     2641 gaggtagctt attcagaatg ctcctataag atagcgagct cgtcaacgat acagatgatc
     2701 tgattatatt gtgcaaaagg agaacaggaa aataggcact ggaaaagatg aaggcctgat
     2761 gggagcatta actaatgcaa aatccgacag gttaatatcc cgaaaagcaa tcaaacgact
     2821 caaacaggag atgcacgctg aagtggtgga caactgttcg gatttcagat gagtagctca
     2881 gcagtttatg agccgaatgc tctgtcacgg gcaggaatca ggctgcatac gcagtgccaa
     2941 atctaatcgt agttattcta accagttttc aagctacaag gtggtgtagc tggcatcttc
     3001 tctactctat catcaaacga taaaatggtt cctctcgtaa catttgctct tgcctttcac
     3061 ttccatctcg atcgcttcgt agttcattac gatagctgac atatttccag tcttcaggtc
     3121 taacatctga cgccggatac tccccgggag cgattttgct tcttaataga ttagactcga
     3181 gacacattat aaattgtcgt attttatgaa gtaataaagc actatattgc gagcattcct
     3241 gatctgattt tacatatagc gtaaactgag ctcctatccc cacacgagat tgctgagaaa
     3301 ctcgattcat atcagttatc ttccatttat ctataggact gtcctctgaa aataataaac
     3361 cagacaaaat ttggaatgcc agaggaactt gctccctagc tatactaata tgaaacttgt
     3421 ccccaacaaa atccccctta ctctgaggat tttcacgaca tgcatgaata aacacatctc
     3481 cactatggtt cacaatctgg aatccttggt acatgacaga aaaaaattga catatatcag
     3541 agtaagttgg cacgttcgat gctactttga aatagtacgc tgggtattgc cgtatttggt
     3601 tgtacaatat attgaagtta tttttcaatc tttcattcgc agaaagcatc tgcggtattt
     3661 cagacctcac aacattcaaa gaatctaaat ttagttttag acagggcttt tttatgggca
     3721 tagaaaacgt cctctataaa atagatatat tatcttatat tgattgttta tcatctttta
     3781 taaaacgata cattatatgt aatgtatgta agcgtaaggg tcttctccga tcatggtggg
     3841 aaggcttaga acgccatatt cagtttccca tagtggaaca tcactcccag cttaaagcgc
     3901 tcccggttgc ggtagccctt ggcttttatt ctcagcagcc tgatcttgct gttcagtgct
     3961 tccgcatttc cgtttgatac gccatggcgc atggcgttca ggataccgta caaccttttc
     4021 cctatcgttt tagccacatt cttcatcatg gggacgtcac tgttggccgc agccatctct
     4081 gccagtcgtt gcgtctttcc tcgctccatg gtctgctcca gatatccttt gccagttctt
     4141 tcagcgccca gcacaggctc gtcagcttca tctgcgcacg cagccatatc agcttttcct
     4201 gcctggattc ggtcatccat ttatcgctgt gctgccacag gaagcgagtt ccctttgcct
     4261 gacgacgact ttcaacagga agatgcggat gttcattctg gcgggtttta tccaccacct
     4321 cacccggttg tttcgccaca tgaaagcgat cgaaggttat tttctcaacc gcattgggta
     4381 agtggatgcg cgctgctctt agtttgctga gccagtttag cattgccttc tacatgaccg
     4441 atgcctgagc cggtataccg aactttattg tcctcaacca gtcatgttat aagcaagaaa
     4501 tgcactcagc ggatagagcg acataatctg aacctgcgga cacatcttaa gcggcttacc
     4561 cgcaaaacca tatgcttctc aaagtctgat gatatgcact acaagatcat cggctggtat
     4621 ctgacaatca accaccacca ctaaatctgc gtcacgacct gcaactgagt ctggttgaac
     4681 aggctgaggc cctgctgaag caacgcgaac ctctcgactc ttgttggatt ttaccaaata
     4741 gtttttctaa aattaaacac atttatataa gttctttgct gaacaaaacg cagcccgcca
     4801 tcctttttca ttcataaatt cagcaatggc agataaaact ttttcatcaa gcatataaaa
     4861 aataatgcca gtagactcaa tatgcctcat acaaataagg ccgcacatat ccgtaatata
     4921 tttgtcaatg ctgctaccat ttgcatggta atatgccatg ctattatctg atgattttac
     4981 atatgcagta tgacatattt ttccttcacg attaacaaaa ctgacaaaac cactttcacc
     5041 aggagtaata tataaagatg aatttaacgt gcgctcacta atattcatta attgttgaaa
     5101 ttcaatggaa tagttttcag ttaatgtatc tattatcttg gctttgtctt tctttgttat
     5161 ctcattgtct ttttcggcaa gaaaaattgc cactttatga catgcattca gctcgttaat
     5221 tttacctgat aaaactgctt ttcttaccac atcgttgaac tcatcgatag tgtgtttttt
     5281 attatatata tcatgaaaaa tatagcctaa agacttaaac gagactttca ttttatattc
     5341 tccttaaata ttaacaggat atatatatca cattttcctc tatattttca gaaaccagct
     5401 atcattttcc ccattactgg tacgtcgctt cagatatttc cgtagcaagc gataaccctg
     5461 cgcactatga ggatgccatt cattataatg tttgaacacc ttcgcgagac tactttactg
     5521 ctgttaacac gtcaggttcg aacataacac tgatttaatc atatttcatc gttttgacga
     5581 agttttctgc cattccattt tgcaccgccg tgtgtttggg ggcaaggccg accatctgtg
     5641 tttcattcgc cagatataag tgatgagatg gcattctccc tccttcacgg cagaagcatt
     5701 ttgaccgttt aggggatgcg gacgattttc atgcctgcta agatactttc gttactgatg
     5761 ctgtccgttt tccaccattt catcatcact gattggcgtt gtgtttttgg tcagcactcc
     5821 agctttacgt ttctctttta accggtaact ctctcctttg atattcagcg tgtagcagcc
     5881 gatctaggat cgctgttgcc agcacgtgat ctccgaacat ttctccccag tctgcgaccc
     5941 ctttgtttga cgtcagtatg atgctcgctt tttcatatcg acagttcatc gataggaatt
     6001 taaatcccca aaagattaaa aaagcaccga agaatgcgcg tttattcaac acaacctgat
     6061 catcaagtaa tcggtataga cacttaatat tgcttacatt gattgattca atgtatctct
     6121 gatggttttt tgctccgttc ccctcaacat ggacgcaact tcccctatgc gactctcagc
     6181 cgcaccaccg tattcgagcc agcggctcat gcatcataac ctgctctgcc agacggtaac
     6241 ccctcttcag ccccgtaaac cgcatctgac ctccgcgcag ctcgcacttc agcgggtcaa
     6301 ccttcagtca ctgctgatac atccctctcc ttgtgatttg cctcgccgtt tttctcactg
     6361 tctccgttat ggcgtacacc acatcttcca gtaatcgccg cttcaccgga ctcaggaccc
     6421 cgtatcacca tccggaaccc cttatccgcc acatgccagg agaacctttc cataaactcg
     6481 tcgccactca tcacaaggtt ttcttctcgt tttgtccggt ggctgttgta acgcaggcca
     6541 atttcatcct gaccggcata atgttccagg cggctcatct gcaccggctg cttctccagc
     6601 cagttaagcc atcggtttcc ctgctgaaaa atatcggcaa aacggggaag catcagaaag
     6661 gcggaataac gccatccggc cagtgaaccg taccgcattc agggcaatac atgccgtcgg
     6721 cgttgatacc ggcaggaatt gtcgcaaatt cccgctccat gcagcgggca atttccggat
     6781 acccttcgtc atcaacacgt acaaaccaga agaccagttt tttgtttctg gcatccacaa
     6841 agaacggaat attcagg
//
LOCUS       DAETPZ010000153         6855 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.155, whole genome shotgun sequence.
ACCESSION   DAETPZ010000153 DAETPZ010000000
VERSION     DAETPZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6855
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.155"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            240..2672
                     /locus_tag="K0W59_003857"
     CDS             240..2672
                     /locus_tag="K0W59_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBI3024687.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGLFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEALI
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="K0W59_003858"
     CDS             2739..4208
                     /locus_tag="K0W59_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBI3024688.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGNAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..5869
                     /locus_tag="K0W59_003859"
     CDS             4208..5869
                     /locus_tag="K0W59_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBI3024689.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6090..6431
                     /gene="symE"
                     /locus_tag="K0W59_003860"
     CDS             6090..6431
                     /gene="symE"
                     /locus_tag="K0W59_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HBI3024690.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6478..6798)
                     /locus_tag="K0W59_003861"
                     /pseudo
     CDS             complement(6478..6798)
                     /locus_tag="K0W59_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2024 a         1625 c         1567 g         1639 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacacccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcctat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcgc
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcaatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt cctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgca cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttgttgtc ggcgctgacg gtgtgaaaat tttatcacct atattaattt gtgagcgttt
     4801 ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa
     4861 agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga
     4921 aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct
     4981 ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac
     5041 cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac
     5101 cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact
     5161 tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga
     5221 aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag
     5281 cgatgaggaa aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat
     5341 agttaatatt aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc
     5401 acctgatatt attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc
     5461 aggagtcaaa gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg
     5521 cccgtcatta tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta
     5581 tccattagac tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt
     5641 tcttatgcaa gtcaaaaaag cagagaacag aataaaaatg ccgaaactta attctgacag
     5701 tttttacaat attattgttg cgattccccc ctataatgaa caacaagcaa tatttgataa
     5761 gataaattct attgaagcag tatgcaatgg tcttatttct tatattggga tttatcataa
     5821 aacccaactg catcttgcag acgcactcac tgacgcagcg ataaactaat tccctcgccc
     5881 tcacaaaacc aataaagcgg ctaaatagcc gctttattca catcaccaaa atttatattt
     5941 cacgctaatt ttcatcttat tttcattaat ttttagctgg actttccctg tatttactga
     6001 tgatatatcc aggtatttag cacggtgcgg atgtgcgcca acacactcgc accgctaatc
     6061 acaatcacta tttatagaga atagtaatca tgactgacac gcattctatt gcacaaccgt
     6121 tagaagcaga agtctccccg gcaaataatc gtcaattaac cgtgagttat gcgagtcgtt
     6181 acccggatta cagccgtatt cccgccatca ccctgaaagg tcagtggctg gaagccgccg
     6241 gttttgccac cggcacggcg gtagatgtca aagtgatgga aggctgtatt gtcctcaccg
     6301 cccaacccgc cgccgccgaa gagagcgaac tgatgcagtc actacgcaag gtgtgtaagc
     6361 tgtcggcgcg taaacaaagg caggtacagg aatttattgg ggtaattaca ggtaaacaga
     6421 aagtcgcctg atacattgac ccggtagcgc cagtgctacc gggtattatt cgccgcgtta
     6481 taccaacgcg ctctccatct gtttttcaaa tgcctgacga attaatccca taatgtaagg
     6541 aagttgtgcg aggtcgctga aaccaatttc tacatcgcca ttaccccagc gcccaacatt
     6601 tgtcacatct ttggcaatac ctttcggatc gaccagttca tgaaactgca tattaagcga
     6661 caatcgcagt cggctttttt gcggcacaac atcaacaaaa ttcgtttccg ctttaaacgc
     6721 aatatatagc ttcaatactt cctgcgtgat ccctgcgtcc aggcgcataa cttcatcgcg
     6781 aagatgatcg aataacactg aggtagcctg agtttaacgg acgctccttc ctgaaataga
     6841 atggcatcag aagga
//
LOCUS       DAETPZ010000154         6785 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.156, whole genome shotgun sequence.
ACCESSION   DAETPZ010000154 DAETPZ010000000
VERSION     DAETPZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.156"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..3524
                     /locus_tag="K0W59_003862"
                     /pseudo
     CDS             1..3524
                     /locus_tag="K0W59_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3534..6785
                     /locus_tag="K0W59_003863"
                     /pseudo
     CDS             3534..6785
                     /locus_tag="K0W59_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
BASE COUNT         1800 a         1781 c         1635 g         1569 t
ORIGIN      
        1 tgtggagcaa gcgaaagata attctttgca accttcccaa acggtaacgg actctctcaa
       61 ggtagcggac ttatccacca ttattcagga acaaccctct ttcgtagcag aaacccgtgc
      121 ggctgacaaa aatacagatg ctgttttacc ttggctggcg aaagatattg ctgtcgtttt
      181 tccgcccgaa gttgtacaca ccacgttaag tcatcgccgc tttcccggag ttcctgttca
      241 gcaagcagac aaattgaccc aattacgtcg cttagcctgt agcgtgtcgc aacgggataa
      301 taaaacagcc acatttgact tcagcacctg ttctttagaa tggcaaaaca ccgtcgccca
      361 agcgatcagt cagatcgacg gcctgaaaac aacacagcta ccatcaccag taatggctgt
      421 actcacggca cttgaaatga aatgcacaag atataaagtg cgtgaggatg ttatggatca
      481 gatcgtccag gaaggcggtc tggaatatgc tactgatgta ataatacacc ttcaacagat
      541 tgatattaaa tgggattatg cgaataatgt cattattatt ctgccgtctg gcattgcacc
      601 tgactacttg gagcaatatt ccagatttga attacgccta cgtaaacatt tatcactggc
      661 ggaagagtct ctctggcaaa aatgtgcaca aaaacttatt gccgcaattc cacatattcc
      721 agaatggcgg caaccattaa ttgctttgtt attacccgaa aaaccagaaa ttgcacatga
      781 aattgcccag cgtctactgg ggcaaaaaaa attaccctcg cttgagtggt taaaaatagt
      841 ggcgactgat gagcacattc ttgcctcatt agaaaaatat cacgagccat atgccatttt
      901 tgatgattac tattgtggtg cgatatggtc agccaccgta ttacaggagc aaggtgttgc
      961 agccctgccc cgatttgctc cctatgccgc aagtgactac tgcgccgatg tgttgcgtca
     1021 tatcaatcat ccgttcgcat taacactgct tatacgtgta gccgggcata ctaaacgctg
     1081 tcacgatcga atgacgaaag cctgcgctgc gttcccacac gcagcactgg cagaacaggt
     1141 cattccctgg ctttcgacac ccgcagttgc agtgctgaaa tcatgccagc accaactgac
     1201 acagccctca aaccatgcca gcgccgatct actgccagcc atagtagtct ccccaccatg
     1261 gatttcgaaa aagaaaaaat cgccgattcc ggtgctggat ttagcgccat taggcatcga
     1321 gccaatctgt tatctgacag aagaaatcag taatcaactt ttggcgaaat atatctggta
     1381 ttcaaaacac atcacggtta gccatgaaga aagtactacc aacctgttgg caaggatggg
     1441 ttttcaacga cggatcgctg gtacatatat taaagctccc gaagcggtag ttgaggcatg
     1501 gctaaatgaa gattattcaa ccttactaag tgaatttaag gtgtttcatt cacctaccgg
     1561 gcattattgg cagttgggga ttttgacaac attgccgctg gaggctggag aaagcagtaa
     1621 aagcatggaa tgcccttact ctatctccac ataccgatac cgaatacgcc atgttacatt
     1681 ttggactcaa aggtttacct ggtttagtta actcaattac acgctatccg caggaagcct
     1741 tgcccatcac gaattacttc gcagcgagtg agctggctcc tgccgtcgcc cgtgccttca
     1801 acaaactcaa aacgctacgc gaaaacgccc gcacctggct gttgaaatac ccggaacatg
     1861 cccttaccgg cctgctgcct gcggcgctcg gcaaagccgg tgaagcacaa gataacgccc
     1921 gcgctgcctt gcgtatgctt accgaaaacg gtcatcagcc attactgcaa gaaatcgccc
     1981 ggcgttataa ccagtcggaa gtaacagacg cggtgaacgc tctgcttgcg ctcgatccct
     2041 tagataatca cccgacaaaa atccccactc ttccgacctt ttatcagcca tcgatctgga
     2101 cgcgcccggt attaaaagca aacgcccaat cactgccaga taacgccctc ctccacctcg
     2161 gcgaaatgct ccgcttccct caggaagagg ctctgtatcc gggattattg caggtgaaag
     2221 acgcctgtac cgctgactct ctggctgaat ttgcctggga tctgtttacc gcctggcaga
     2281 ccgctggcgc gccgtcgaaa gagagttggg cgttcactgc gttaggcgtt ctcggtaacg
     2341 atgacaccgc ccgcaaactg acgccattaa tacgcgcctg gcctggtgaa tcccagcata
     2401 aacgcgccac cgttgggttg gatattctcg ccgctatcgg tagtgatatc gccctgatgc
     2461 agcttaacgg cattgctcag aaactgaaat tcaaagcatt acaggagcgg gcaaaagaaa
     2521 agattgccga cattgccgag agccgcgaac tcacggtagc ggagcttgaa gatcggttag
     2581 caccggatct cggtctggat gataacggtt cgttgctgct ggattttggc ccacgccagt
     2641 tcaccgtcag ctttgatgaa accttaaaac cgtttgtgcg tgatgtttcc ggcagccgcc
     2701 tgaaagacct gccaaaacca aacaaaagcg atgatgagtc acaggcgaac gatgctgtta
     2761 accgctacaa attgctgaaa aaagatgcgc gtaccgtcgc cgcccagcag gtagcaaggc
     2821 ttgaatccgc catgtgcctg cgtcgccgct ggtcgccgga aaacttccaa ctcttcctgg
     2881 ttgtgcatcc gctggttcgc cacttaaccc gccgtctgat ttggggcgtt tatagcgccg
     2941 acaaccagct actggcctgt ttccgggtag cggaagataa cagctacagc accgctgacg
     3001 atgatctttt caccctgccg gaaggcgata tctctatcgg cattcctcac gttctggaaa
     3061 tatcaccgac ggatgctgtc gcctttggtc agctttttgc cgactacgaa ctgctaccac
     3121 cgttccgtca gcttgaccgt aacagttacg ccctgacaga agccgagcgc aacgccagcg
     3181 aactgacccg ctgggcaggc agaaaatgcc cgagcggccg ggtcatgggg ctggcaaata
     3241 aaggctggat aaagggcgaa ccacaggatg gaggctggat cggctggatg atcaaacctc
     3301 tcggtcgctg gtcgttaatc atggaaatcg acgaaggttt tgcggtgggt atgtcaccag
     3361 ccgaacttag cgctgagcag ctcttaagca agctgtggct atgggaaggc aaagcggaaa
     3421 gctatggctg ggggagtaat tcaacacagg aagcgcagtt ctccgtactc gatgccatca
     3481 ccgccagcga gctaattaac gatattgaag ccctgtttga ataaggaaag agcatggaca
     3541 aggaattacc gtggctggcg gataacgccc aactggaact gaaatataaa aaaggcaaaa
     3601 caccactcag tcatcgcaat tggccgggcg aaccagtgcc tgttatcact gaaagcatca
     3661 ttcagacact gggtgatgaa ttgctacaaa aagctgagaa gaaaaaaaac attgtctggc
     3721 gttatgaaaa tttttcaccg gagtggcagt ccgccattac gcaggccatc aacttgatcg
     3781 gcgaacacaa accctcagtc ccggcccgca caatggcggc actagccttt atcgcgcaaa
     3841 atgacaacca acagttgctc gacgaaatcg tccaacaaga ggggctggaa tatgcgactg
     3901 aggtggtgat tgcacgccag tttattgcac ggtgttatga gagtgatcct ctggtagtca
     3961 cattgcagta tcagaacgag gactatggct atggttatcg ctcagaaacc tataacgaat
     4021 tcgatctccg actgcgtaaa cacctctctc tggcagagga aagctgctgg cagcgttgcg
     4081 ccgacaaact cattgccgca ctgccaggaa taaccaaagt tcgccgccct tttattgcgc
     4141 tgatcctccc cgaaaaacca gaaatcgcca atgagttggt aagccttgaa tgcccgcgga
     4201 ctcattttca ttctaaggag tggttaaaag ttgttgctac tgaccccaaa gcggtgagaa
     4261 aactcgaacg ctactggagc caggatatat ttagcgatcg agaagccagc tacatgtcgc
     4321 atgaaaacca cttcggctac gcggcctgcg ctgccctttt gcgcgaacaa ggactggcag
     4381 tcgttccgcg cctcgcgata tatgcccata aagaagattg cggcagtctg ctggtacaaa
     4441 ttaaccatcc gcaagtcatc cgcaccttgc tgttagttgc tgataaaaac aaacccagcc
     4501 tgcaacgtgt ggctaaatac agtaaaaact tcccccatgc gacactcgcc gcactggcag
     4561 aactgctggc gttaaaagaa ccaccagccc gcccaagtta tccaatcatc gaagacaaaa
     4621 agctgcctgc acagcaaaaa gctcgagatg aatactggcg tacgctgtta caaacgctga
     4681 tggcatcgca gccacaactg gcagcagaag tgatgccgtg gttaagtact caagcccggg
     4741 cagtgctgaa tagttattta tcggcaccgc ccaaaccagt tattgatagc accgataaca
     4801 gccaaatgcc tgaaatcctc gtttcaccac catggcgtgg gagaaagaaa aagacttcca
     4861 tacgtcactt ttcgctgcct gatcttacgc ttgcaccgca aacgtacagg tcgccgaata
     4921 aacactggac gccggaacag caaactgcaa ttgaccattt tagcgccatg tcttttgacg
     4981 aacgtcttgc tacgagagga gcggatatct tcttgcggga attaggtttt gacgttcata
     5041 gcctggaatt taataagcgt cttcttaatg gacagttgga tgcctttgat gctcagcaac
     5101 tgcaagaata cacatcaata ggactattgc gtacttatca cgcgcatcag gccaaatcta
     5161 aacagtaccg ggaagacgcc accaatgcat tactggcaca agatagcatt gcgttgatgg
     5221 gagggtggcg aacttttcag cacggatttt actatggtaa tgaacagcgt tgggggattt
     5281 ggaatctgta ccttatcgcg cagatgccgc gcgaaatggc ggtatcgtgc tggcaacaaa
     5341 ttgtcgaggc tgattttcat tacaccgggg tggaatattt gttaagtgtg ctgggtaccg
     5401 atgctttgcc tggattaaac accgcctttg cccgacatcc aaaagagata tttccgctgc
     5461 tgattcattt cggcgcaaca gaactggccc tgcccattgc ccgcgtctgg cacctttttg
     5521 cgggccagcg taatctggct cgccagtgga ttttacaatg gccggaacat acggctactg
     5581 cacttatccc tcttgtcttc accaaatcta gcgataaaag cgaagctgca ttacttgccc
     5641 tgcgtttact ttacgaacat gggcatggcg aattgctaca aactgtagcc aatcgttggc
     5701 agcgtacaga tctatggcct gccctggaac aattacttaa acagagtcca atagagattt
     5761 acccggcacg cattccaaaa gcccctgatt tctggcatcc gcaaatgtgg tccaggccgc
     5821 gccttatcac taataatcaa cctgttaccg atgacgctct ggaaattatc ggcgaaatgc
     5881 tgcgctttac ccagggggga cgtttttata gcgggctgga acaactgaaa acgttctgcc
     5941 agccacaaac gctggcagct tttgcctggg atctcttcac tgcgtggcaa caagctggtg
     6001 cccccgcaaa agacaactgg gcatttctgg cgttaagtct ctttggtgac gaaagcacgg
     6061 cacgggatct aacgacacat atcctcgcct ggccacaaga aggcaaatct gcccgtgcgg
     6121 tcagtggcct gaacatcctt accctgatga ataatgatat ggcgctgatg cagctgcatc
     6181 atatatcgca acgggcgaaa tcccgcccct tacgtgataa cgcggcggaa tttcttcagg
     6241 tagtcgcaga aaatcgcggg ctaagccagg aagagttagc ggacagatta gtcccaacac
     6301 tgggccttaa tgatccgcag gcgttgagtt ttgattttgg tccccggcag tttaccgttc
     6361 gcttcgatga aaacctcaac ccggttatct ttgatcagca aaacgttcgc cagaaaagtg
     6421 ttccccggtt gcgcgccgat gacgatcaac tgaaagcgcc cgaggcactg gcccgactaa
     6481 aagggttaaa aaaagatgct actcaggtga gcaaaaacct gctcccgcgt cttgaagctg
     6541 ccctacgtac cacccgacgc tggtcgctgg cagattttca ttctctgttt gttaatcatc
     6601 cctttacccg tctggttacc cagcgattaa tatggggggt tatccggcaa atgaaccgcg
     6661 tcgtttactc aacgcctttc gtgtggccgc agagggggag ttctgcaatg cgcaagatga
     6721 gccaattgac ctgcctgcgg acgctctgat tggcattgcc cacccgttag aaatgacagc
     6781 agaaa
//
LOCUS       DAETPZ010000155         6780 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.157, whole genome shotgun sequence.
ACCESSION   DAETPZ010000155 DAETPZ010000000
VERSION     DAETPZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.157"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            337..579
                     /locus_tag="K0W59_003864"
     CDS             337..579
                     /locus_tag="K0W59_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024691.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAVTGGGDGSNTGTTTTTTTSTQ"
     gene            693..1331
                     /locus_tag="K0W59_003865"
     CDS             693..1331
                     /locus_tag="K0W59_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HBI3024692.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQSTLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1328..2065
                     /locus_tag="K0W59_003866"
     CDS             1328..2065
                     /locus_tag="K0W59_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBI3024693.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPFTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            2065..4161
                     /locus_tag="K0W59_003867"
     CDS             2065..4161
                     /locus_tag="K0W59_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBI3024694.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ERMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4208..4486)
                     /locus_tag="K0W59_003868"
     CDS             complement(4208..4486)
                     /locus_tag="K0W59_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024695.1"
                     /translation="MKRNLIKVVKMKPYFATLMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4487..4630
                     /locus_tag="K0W59_003869"
     CDS             4487..4630
                     /locus_tag="K0W59_003869"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024696.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4756..5166
                     /gene="psiE"
                     /locus_tag="K0W59_003870"
     CDS             4756..5166
                     /gene="psiE"
                     /locus_tag="K0W59_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HBI3024697.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5210..6684)
                     /gene="xylE"
                     /locus_tag="K0W59_003871"
                     /pseudo
     CDS             complement(5210..6684)
                     /gene="xylE"
                     /locus_tag="K0W59_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
BASE COUNT         1698 a         1696 c         1785 g         1601 t
ORIGIN      
        1 aatgccgaca ataagtcggc attgttttat cagataaatc cccttgtctg tgatttaacg
       61 gaaatcatac cgtgaggtta atcctaaaat agatttttaa tcgttgttta tttcggagaa
      121 tacgcagatt aattactttt gtttttattt taagtttatg atttttattg ttatttaaat
      181 ataagttgaa acttatattt gatattcatt ccaattatcc taaaactcca tcgctaattc
      241 ctcgcgccgt aattcgcatg ctttagttgt gtatactcga tcccgcccga aatgtttttg
      301 ggtaaatctc cattcattca atgaagggaa attgttatga aaaaagttct gtatggcatt
      361 tttgccatat ctgcgcttgc ggcgacttct gcgtgggctg cacctgtaca ggtgggcgaa
      421 gcggcagagt cggcagcaac gtcggtttcg gcggggagtt cctccgcgac cagcgtcagc
      481 accgtaagct cggcggtggg tgtcgcgctc gcggtaaccg gtggcggtga tggttctaat
      541 accgggacca ccacaaccac gaccaccagt acccagtaat aaagtatgta tccccaaaat
      601 aatttgagtc attgcatctg tggcttgaag tatgaaggga tttaccataa ccacactccg
      661 gtgtggttat tctgcccctc tggagaagag tcgtgaagcg acctgcactc attcttatct
      721 gcctgctatt acaggcctgt tcagccacga ctaaagagct gggcaattca ctgtgggaca
      781 gtctgttcgg cacgccaggc gtacagctga cggacgatga tattcaaaat atgccctacg
      841 ccagccagta catgcagctt aatggcgggc cgcaattgtt tgtggtgctg gccttcgctg
      901 aagacggaca acaaaaatgg gtcactcagg atcaatccac tcttgtcaca caacatggtc
      961 gtctggtgaa gactttgctt ggcggcgaca acctgattga agtgaataac cttgccgccg
     1021 acccgctgat taaacccgca caaattgttg atggtgcaac atggacgcgc acgatgggct
     1081 ggaccgagta ccagcaggta cgctacgcca ccgcacgctc agtcttcaaa tgggatggca
     1141 ccgataccgt caaggtcggc agcgatgaaa ccctggttcg cgtgctggac gaagaagtct
     1201 ccaccgacca ggcgcgctgg cataaccgct actggatcga cagcgaaggg caaattcgcc
     1261 agtcggaaca gtatctcggc gcggattatt tcccggtgaa aaccactctc atcaaggcgg
     1321 caaaacaatg attaaacaaa ctattgtcgc gttgcttttg agtgtgggag cgtcatcggt
     1381 cttcgcggca ggaaccgtca aggtgttcag caatggcagc agtgaggcca aaacgctaac
     1441 gggcgcagag catttaatcg atctggtagg gcaacctcga ctggcaaaca gctggtggcc
     1501 tggtgcggtg attagcgaag agctggcaac ggcggcagca ttgcgtcagc agcaggcgtt
     1561 gctgacacgg ctggcagagc agggcgcaga ttccagcacc gacgatgccg ccgccattaa
     1621 cgccttacgc cagcaaatcc aggcattgaa agtgacaggc aggcaaaaaa tcaatcttga
     1681 tcccgatatc gtccgcgttg ccgaacgcgg taacccgccg ttgcagggca actacacgct
     1741 gtgggtcgga ccaccgccgt tcacggtcac gttgttcggg cttatcagcc atcctggcaa
     1801 tcagccattc actccaggtc gcgacgtggc gagctatctc tctgaccaga gcctgctcag
     1861 cggtgcggat cgcagctacg cgtgggtcgt ttacccggac ggacgcacgc aaaaagcgcc
     1921 ggtggcttac tggaacaagc gtcacgtaga gccgatgccc ggcagcatta tttatgttgg
     1981 cctcgcagat tccgtctgga gtgagacgcc tgatgccctt aacgccgaca ttcttcagac
     2041 tctgacgcag cggatacccc aataatgaaa aaaagacatc tgcttagctt actggcgctg
     2101 ggcattagca cagcttgcta cggcgaaaca tatcctgcgc ccattggtcc gtcgcagtcg
     2161 gatttcggtg gcgtaggatt attacaaact cccaccgagc gtatggcacg ggaaggggag
     2221 ttgagtctga actatcgcga taacgatcag taccgttatt actcagcttc agtgcaactc
     2281 ttcccgtggc tggaaacaac gctgcgctac accgacgtgc gcacccggca gtacagcagc
     2341 gtcgaagcgt tctctggcga tcaaacgtat aaagataaag ccttcgatct caaactgcgt
     2401 ttgtgggaag agagttactg gctgccgcaa gtggcggttg gtgcgcggga tatcggcggt
     2461 acggggctgt ttgatgcgga atatcttgtt gccagcaaag cctgggggcc gttcgatttt
     2521 acgctcggcc tgggctgggg atatctgggc accagcggta atgtaaaaaa tccgctctgt
     2581 tcagccagtg ataaatattg ctatcgcgat aacagctaca aacaggcggg atctatcgac
     2641 ggtagccaga tgttccacgg tcctgcctca ctgtttggcg gcgtggaata ccagacgccc
     2701 tggcaaccgc tgcgtctgaa actggagtat gaaggtaata attatcagca ggattttgcc
     2761 gggaagctgg agcaaaaaag taagtttaac gtcggggcga tttatcgcgt taccgattgg
     2821 gccgacgtta accttagcta tgaacgtggc aacaccttta tgtttggcgt cacgctgcgc
     2881 accaacttta acgatctgcg cccgtcctac atcgataacg cccgcccgca atatcaaccg
     2941 cagccgcagg atgccatttt gcagcattcg gtggtggcga atcagttaac gttgctgaaa
     3001 tacaacgccg gacttgccga tccgcagatc caggcgaaag gcgatacgct gtatgttacc
     3061 ggcgagcagg tgaaatatcg tgattcgcgc gaagggatca tccgtgctaa tcggatcgtg
     3121 atgaacgatc tgccggatgg gatcaaaacg atccgcatta cggaaaatcg ccttaacatg
     3181 ccgcaagtga cgacggaaac cgatgtcgcc agcctgaaaa atcatctcgc cggagagccg
     3241 ttgggccacg aaacgacgct ggcgcacaaa cgcgtcgagc cagtggttcc gaagtccacc
     3301 gagcagggct ggtatatcga caaatcacgc tttgatttcc atatcgatcc ggtgctgaac
     3361 cagtcggtcg gtggcccgga aaacttttac atgtatcagc tgggcgtgat gggaacggca
     3421 gatttgtggc tgacggacca tctactgacc actggcagcc tgtttgcaaa tcttgccaac
     3481 aactacgaca agtttaacta caccaatcca ccgcaggact cgcacttacc gcgcgttcgt
     3541 acccatgtgc gcgagtatgt gcagaacgat gtctatgtga ataacctgca agccaactac
     3601 ttccagcatc tgggcaacgg cttctacggt caggtctatg gaggttatct cgaaaccatg
     3661 tttggcggtg cgggggccga agtgttgtat cgtccgctgg acagtaactg ggcgtttggt
     3721 ctggatgcca actacgttaa acagcgcgac tggcgtagtg caaaagatat gatgaaattc
     3781 accgactaca gcgtgaaaac cggacatctg accgcctact ggacgccatc tttcgcccag
     3841 gacgtgttgg tgaaagccag cgtcgggcag tatctggcag gggataaagg cggcacgctg
     3901 gagatcgcta aacgctttga cagcggcgtg gtggtgggtg gctatgccac gatcaccaac
     3961 gtttcgaaag aggagtacgg cgaaggggac ttcaccaaag gcgtgtatgt ctcggtaccg
     4021 ttggatctct tctcgtctgg cccgacacgc agccgtgcgg cgattggctg gacgccgctg
     4081 acgcgtgacg gtggtcagca acttgggcgt aagttccagt tgtatgacat gaccagcgac
     4141 cgtagcgtaa atttccgcta agtcatggga aaggtgccag ttttcgcact cactggcacc
     4201 ctcattctta atgacctgtt gagccatcag gaacgcagtt cacccccggt ttacgatctg
     4261 gtgtgtaaag gcgaaaacat tctgtcttcc tgtccgtcga tatcggttgc aggtctggtt
     4321 gcctgagcga ctgggccatc attgggctgg agacattgct ttgcggtagg tcggctttat
     4381 cagcagtacc gagcggacca gcataagcag gaagaacaga gactgataac atcaaagtag
     4441 caaaataagg cttcattttt accaccttta tcaggttacg tttcatttgt tccagaggaa
     4501 cattgtcgat ttttcgcgca ttgctggtgg ctgggaatca cctgaatggg tgatttttga
     4561 attaccggct ttggtgcggt ttgtcttgcc ggatgcgccg ccaggcgcgg cttatccggc
     4621 ctacgggtag gtatatccgg ctttggtgga ggcgcgctcc aaatccaggt tgaacaaaac
     4681 atacacaaaa aatatagatc tccgtcacat ttttgcgtta tacaggaagc tcgccactgt
     4741 gaaggaggta ctgctatgac gtcactctct cgtccgcgcg tggagtttat ctccaccatt
     4801 ttgcagaccg tactcaatct tggcctgctg tgcctcggcc tgattttggt tgtcttcctc
     4861 ggcaaagaaa cggtgcatct ggctgatgtg ctgttcgcgc cagaacaaac cagcaaatat
     4921 gagctggtag aaggactggt ggtttacttt ctctatttcg aatttatcgc gctgattgtg
     4981 aagtactttc agtccggttt tcacttcccg ctacgttact ttgtctatat tgggatcacc
     5041 gccattgtgc gcttgatcat cgtcgatcat aaatcgccac tggatgtgtt gatctactcg
     5101 gctgcgatcc tgttactggt gatcaccctg tggctgtgta attcgaaacg gctgaagcgg
     5161 gagtaaaaag acagcacgcc gaaatggcgc ggcgtgctgg acaggaagat tacagcgtag
     5221 cagtttgttg tgttttcttc gtttccggtt cccagagcgc ttccagctcc tcaagggttt
     5281 tacctttggt ttccgggaca aatttccaca taaacagtgc tgccagaacg cccatacaac
     5341 cgtaaatcca gtaggagaaa ccgttgtgga aatgggccac cagccaggag tttttgtcca
     5401 tcatcgggaa ggtccaggag acgaagtagt tcgccagcca ctgggccgcc accgcgattg
     5461 ccagcgcttt accacgaata gcattcggga agatttccga cagcagtacc cagcataccg
     5521 gaccccagga catggcaaag gcggcaacat agaacagcat cgacagtagc gccacaatac
     5581 ccgagcctga gtgtaaaacg cggtactgag gctaaacata ccgattgcca ttccgagtgc
     5641 gccgataatt tgcagtggct tacgaccaaa tttatccaca gtcataattg ccagtacggt
     5701 aaaggtgagg ttgataactc cgacaataat ggtctgcaac agcgcgatat ccgtgctggc
     5761 ccccagcgtt ttgaacactt ccggcgcgta gtacagcacc acattgatgc cgacaaattg
     5821 ctggaagatg gagagcataa cgccgattac aatcacgccc acgccaaaca tcagcagacg
     5881 accaccggtt ttgcggccat gatccaggga gtgtttaatt tcctgtactg cctgagttgc
     5941 aagcgtgttg cccataattt tgcgcaggat actttccgtc tgttcttgct tgccgcgtga
     6001 catcagccag cgaggacttt ctggcacggt atacagcagc attaagaaca gcagtgcagg
     6061 gatacattcc gaggcaaaca tataacgcct gccgtcagta ttcagccagc tggcatcacc
     6121 ggaacgggca ataaaatagt ttacgcagta aactaaaagt tgcccgaaaa taatcgcaaa
     6181 ctggttaaat gaaaccagtt ttccgcgaat atgagctgga gccagttccg caatatacat
     6241 tggtgaaagc attgaggcta aaccaacgcc aataccgcca ataatacgat aaataacaaa
     6301 ttccgggaca taacctgcca gataaatagg tactgtgttg tccgggttta tagaggtaaa
     6361 accaagttct ggccaggcag aacctacacc agaaataaaa aacaggacag cagaaatctt
     6421 aagtgaatca cgacggccga agcggttact gcaataacca ccgagggcac cgccgatgat
     6481 gcaaccaatc agagcgctgg ctacgcaaaa tcctaacagg gagttggcag cggattcact
     6541 taagttttgt ggagcaacaa agacggtatt gagtgactca acagtaccgg aaataacggc
     6601 ggtgtcgtag ccaaataata aaccacctaa tgtagcgact aaggtaatcg aaaatatata
     6661 actggaacta tactgggtat tcattcagac ctgccttaga ccattctgat cttttatcag
     6721 tgcattgatg ttggaattgt caggcagtta aggcaattat cattttttac aatgcgttta
//
LOCUS       DAETPZ010000156         6779 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.158, whole genome shotgun sequence.
ACCESSION   DAETPZ010000156 DAETPZ010000000
VERSION     DAETPZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.158"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..92)
                     /locus_tag="K0W59_003872"
     CDS             complement(<1..92)
                     /locus_tag="K0W59_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleE"
                     /protein_id="HBI3024698.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRV"
     gene            148..300
                     /locus_tag="K0W59_003873"
                     /pseudo
     CDS             148..300
                     /locus_tag="K0W59_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1681..2628
                     /gene="icsP"
                     /locus_tag="K0W59_003874"
     CDS             1681..2628
                     /gene="icsP"
                     /locus_tag="K0W59_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HBI3024699.1"
                     /translation="MKSKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3190..3562)
                     /locus_tag="K0W59_003875"
                     /pseudo
     CDS             complement(3190..3562)
                     /locus_tag="K0W59_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3806..3925
                     /locus_tag="K0W59_003876"
                     /pseudo
     CDS             3806..3925
                     /locus_tag="K0W59_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4175..5041
                     /gene="ospB"
                     /locus_tag="K0W59_003877"
     CDS             4175..5041
                     /gene="ospB"
                     /locus_tag="K0W59_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HBI3024700.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5370..6110
                     /gene="phoN2"
                     /locus_tag="K0W59_003878"
     CDS             5370..6110
                     /gene="phoN2"
                     /locus_tag="K0W59_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HBI3024701.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(6463..>6779)
                     /locus_tag="K0W59_003879"
     CDS             complement(6463..>6779)
                     /locus_tag="K0W59_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HBI3024702.1"
                     /translation="KKADIALYLLSKFNFIKQDIAEMEKMNNNIYCNLYDVEYLLSKD
                     GANYKVLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAVSGKRF
                     ER"
BASE COUNT         2131 a         1268 c         1315 g         2065 t
ORIGIN      
        1 tcaactctag gataaatatt tctcactata tattcagtat ttgcagtatt tattggaaat
       61 acgttcttaa tattcttgat gggactaatc attttaatct ctatactcaa atataaacat
      121 taccatgaac aatataattg ttcctaagtg gaatgtctcc acggagaaca ctttatctat
      181 cgggaaatag tgcgggcaac agtgtttaat tatatcgagt gcgattacaa tcgctggcga
      241 cggcaccgtt ggtgtggcgg cctcagtccg gaacaatttg aaaaccagaa cctcgcttag
      301 gcctgtgtcc atattacgtg ggtaggctca cagagcctca cttacgactg cgtccatatt
      361 atgtgggtag gatcaataaa gacgttttat atgtgtctgc ttgcagccag aaaatttata
      421 ccctgcaggt atcataaaca tgcttgataa cttaattggg gctccctctt ccttttatgg
      481 tgtaattgtt tgattgaata cttccgggga tttcagtatg aaatgaagta tatttaatat
      541 actttacaaa aagtttgtat tagtggaata aggacaatag cttatccaaa ctgccaagcc
      601 tctttattat aagtaagatc tggcactgga atattctcaa aaggactaac atcatcacca
      661 cgaccttcaa ccaaccattt tattgcattt aaatcccaga atagttcagt agtggtcaat
      721 ttgggaaacg cacaatattg tacggttacg ttatcgcaat agagtgaact cccgccctat
      781 acggcgtttt caaggattag tccttaatcg gacaaccaca ttaaagcata ataaataatt
      841 aagctttaat aatatactat agatacagtt ctattataaa aaatactatt ttaggtgatg
      901 cattaaatta gtagaatatt tattctttta taaaagcacc aatgaattgt ccaagtaaac
      961 atgaggtaaa atgtgtagag gataactgag tttggtcact ttaactgtat tagtcgcagt
     1021 ctgactctcg actttaaaag gatgggaatt acctgtactg atagtgagaa agctttccat
     1081 tgaaataagt caaataaaag ttgatggctt cagtgctaaa gtgaggtgcc cgctagccgt
     1141 attcactcga atcggaattc aatgtattgc ttctgccatt tccataatat ttcacgggaa
     1201 aaacaaaatc tgaaaaacta tacttaagtg ttgtattgag tcagttgaga tacaaataca
     1261 tatttatcag cacacgttac gtcaaataca ggaggttgtc atgttggtac tgaaaggcac
     1321 gttggctatt gcagccgtga tgacaattta ttgtcttatc gttgttctta tggaacgctt
     1381 ttctgattga tttcatattg gcgagagaac aagaaggtga gtagaattgt cgcggaatcc
     1441 tgaaactatc agcctcaggt atgaacacca aaaggtaggt agagaaatct atggcccccg
     1501 ttactttatt cgcacaatga ggtatttttt tatttttaca aaagttcaat aaatattata
     1561 ataaaagata tgttcttggt aacataaaca aatatttaat taaaataatg cttggcattg
     1621 acgtttatct tataaagtaa gaagatcatg gacatttcaa ccaaaaaagt agagttctcg
     1681 atgaaatcaa aattccttgt acttgcactt tgtgtacctg cgatctttac tacacatgct
     1741 accactaact atccactttt cataccggac aacatcagta cggatattag tctaggatct
     1801 ctgagtggca aaacaaaaga acgcgtttat catcccaagg aaggggggag aaaaattagt
     1861 caactggact ggaaatacag taatgcgact attgttagag gtggcatcga ttggaagcta
     1921 attccaaaag tgtctttcgg agtttccggt tggactactt taggtaacca gaaagcaagc
     1981 atggttgata aagactggaa caattccaat acccctcagg tatggaccga ccagagctgg
     2041 catcccaata cgcatctccg tgatgctaac gaattcgagc tgaatcttaa aggttggtta
     2101 ttaaataatt tggattatcg acttggacta atagcaggtt accaagagag tcgttacagt
     2161 tttaatgcaa tgggagggag ttatatttat agtgaaaacg gggggaacag gaataaaaaa
     2221 ggggcacatc ctagtggtga aagaacaata ggttacaaac agctcttcaa aataccttat
     2281 attggattaa ctgctaatta ccgccatgag aattttgagt ttggagcaga actgaaatat
     2341 agtggttggg ttctttcatc tgatacagat aaacactatc agactgagac aatttttaaa
     2401 gatgaaataa aaaaccaaaa ttactgctct gttgctgcga atattggata ctatgtcacc
     2461 cccagtgcaa aattttatat agaaggctcc agaaattaca tttctaataa aaaaggtgat
     2521 acatctcttt atgagcaaag taccaatata tctggcacca ttaaaaatag tgcaagtatt
     2581 gaatatattg gttttctcac ttccgcaggt ataaagtata ttttttgaat tggttcaccg
     2641 agttgattac gttttcatgg ctaaaaattg ccgcccattg tgagtcatat tcgccctgac
     2701 tttccagaac catacgaact gcccgttgac ggacttcggg ggaaaaacgt gtatttttag
     2761 tcattctgtt tacctctttc tcagggcgtt tagtctccag gatttccggg gcggttcaaa
     2821 aaactcttcg ttgattactg tggacttact gttggcatta ccgaccctga gaccggagaa
     2881 ataagaaccg cttagatcat cgtggctgtt ctcggggtat caagttacac atgggcagag
     2941 gctacccaag gtctactatt atcgcctgaa agcgctgatg gaacagtgct atcagggaca
     3001 tgctgatgaa aatacctaaa taatcagcca gcactctatc tttccaaatc cacagcatag
     3061 caaagagagc aaaagagcct gtaaattcag aaattaagga aacgcctctt ggtgcacata
     3121 atctatctta tgttaaatga aatctaattt cttgattata tgctggatcg ttcgtgggtg
     3181 gctttgcccc tatatttcca gacacctgtt atcattggcc cgctgccgca gatattcccg
     3241 tggcgagcga taatccagcg cactatgcgg atgccattcg ttataatgct cgaatgcctc
     3301 tgcaaggttc tttgctgccg ttaacccgtc aggtttgggc atgatactga tgtagtcaca
     3361 ctttattgtt ttcacgaagc tctttgctat tctgttactc tccggactcc gcaccgccgt
     3421 gttcttcggt tcaacaaagt cccaacatcc gggtgaactg gcgtgtttca ttagcccggt
     3481 agcatgaacc attatccgtc agtcactcca ctggagacga cggaagatcg ttgccgaagc
     3541 ggtgttccac cgctcccagc atgtcgccaa tgggagagca tctacggtct gattgaactg
     3601 tttattcaaa ttgtccaccg tctgtcagta cgctctgaac gtcggctggt taaaacactg
     3661 ctcgctgatt ttcagagggt tcacggtaaa acagcacttt tattccggat tgcggaagcg
     3721 gctctgaata atccggatgg gctggttaaa gaggttgtgt accatctgca tattgttgaa
     3781 ccagatcgca gcggtaagag aagttcatca tacttggcgc agttacgcga tgtttctgct
     3841 agaggtgatg ccgtaaagaa cggcagaaca ttaccggaac aagatagcgg tctacctgca
     3901 ctggtatcag acccggggct tccccggatg atctccacgg tactttaggt actgtgtcta
     3961 tattatgtag gtaggatcaa ttaactttgt agttgaaatt ctttcattga aataatgaat
     4021 aaaaaagaca aaccgcatta aacaatgcca gaatataatt gcatttttgt tccgtttttt
     4081 taaatcatag tatacaaaga agcttgcata ctatatgaaa atataacacc ctttactatg
     4141 atgtttataa aacaatatat ggagtcatgt aggtatgaat ttagatggtg ttagaccata
     4201 ctgtagaata gtcaataaaa agaatgaaag catatcagat attgcatttg cacatataat
     4261 aaaaagggta aaaaattcat catgtactca cccaaaagca gcattggttt ttttaggaga
     4321 gaaaggtttt tgtgatagca atgatgttct atctattatg ggacaacaaa taccaagagt
     4381 atttaagaac aagatgttat atgattatgt ttttaaaaat gaaaaaagta aaaatgattt
     4441 tctaaaaatg gctgaatcat ggctaccaca gagtgaacca atagtaataa ataatgatga
     4501 tgacgcattg aatgctgctg cttatttttc tgtaaaaaaa gcgaaaataa aaacagtaaa
     4561 cgatactgat tttaaagagt ataataaggt ttatattctt gggcacggta gtcctggttc
     4621 tcatcaatta ggccttggtt cggaacttat tgatgtacaa acaatcattt caagaatgaa
     4681 agactgtggt attctaaatg tgaaagatat ccgttttact tcatgcggct ccgctgataa
     4741 agtggctcct aaaaatttta acaatgcccc tgctgaaagt ctttcttgta tccttaactc
     4801 tctgcctttt tttaaggaaa aagaatcttt gctagagcag ataaaaaaac accttgaaaa
     4861 cgatgagtca ttgagtgatg gtctaaaaat atccggctat catggatatg gagttcacta
     4921 tggtcaagag ctttttccct actcacatta tcgttcaact tcaattcctg ctgatccgga
     4981 gcatacagta aaaagaagct ctcagaaaaa gacttttatt attaataaag aactggatta
     5041 gtataaaatt tttaacctat agttaattgt ttgcatttaa gatgaagtta ttttatcttt
     5101 agtagaaaag tgcttcccta tgagggaaaa tcacaaatca tcataataca gagacaaaac
     5161 gatacgaaca aatagataaa aaacatcatt cttttaccac attatttttc gtgaatatga
     5221 aaaataatgt tattacttta atataagact attttttgtt tttccatcac tctgttcaaa
     5281 tttttccgca tgacttgtgt tttttgtaac acagctcgtt ttttacagct gaccaaaatc
     5341 atcaattaat tatgctaagg aaataaatta tgaaaaccaa aaactttctt cttttttgta
     5401 ttgctacaaa tatgattttt atcccctcag caaatgctct gaaggcagaa ggttttctca
     5461 ctcaacaaac ttcaccagac agtttgtcaa tacttccgcc gcctccggca gaggattcag
     5521 tagtatttca ggctgacaaa gctcattatg aattcggccg ctcgctccgg gatgctaatc
     5581 gtgtacgtct cgctagcgaa gatgcatact acgagaattt tggtcttgca ttttcagatg
     5641 cttatggcat ggatatttca agggaaaata ccccaatctt atatcagttg ttaacacaag
     5701 tactacagga tagccatgat tacgccgtgc gtaacgccaa agaatattat aaaagagttc
     5761 gtccattcgt tatttataaa gacgcaacct gtacacctga taaagatgag aaaatggcta
     5821 tcactggctc ttatccctct ggtcatgcat cctttggttg ggcagtagca ctgatacttg
     5881 cggagattaa tcctcaacgt aaagcggaaa tacttcgacg tggatatgag tttggagaaa
     5941 gtcgggtcat ctgcggtgcg cattggcaaa gcgatgtaga ggctgggcgt ttaatgggag
     6001 catcggttgt tgcagtactt cataatacac ctgaatttac caaaagcctt agcgaagcca
     6061 aaaaagagtt tgaagaatta aatactccta ccaatgaact gaccccataa agctggacag
     6121 cctgtatcag gctatggagg gcccatagac aaatctaccc tatatgagca taggaggagt
     6181 ctatgggcac accacgtttt acccctgaat ttaagggatt actggaaagg ctgggacata
     6241 tccctccggc agaagcagaa aaagcttatt atgctgccat cggaaacgat gatctggcaa
     6301 cctgagttca cagataaaac attctctagg aaactcgggg cggttccgtt caccacatgc
     6361 aatgtggtgt tgcaggggaa cggtctgcac catcccctat atcgatcaat ataacaaaaa
     6421 tgacaacttc agattcaggg cacaacctaa atatatttta ggtcacctct caaatcgttt
     6481 gcctgatacc gctccatttt ttaataaaaa atcaatcatt tttgaatctt tgcttttcat
     6541 tgcattgtca agcatagtgt ccccactatt tgctttttga aattttttgt ttacatcaac
     6601 caatccatta ttgataaaat attctagcac tttatagttg gcaccatctt tactaagtaa
     6661 gtattcaaca tcatacagat tacaatatat attgttgttc attttctcca tctctgcgat
     6721 atcttgtttt ataaaattaa atttagatag taaataaagt gctatatcag cttttttat
//
LOCUS       DAETPZ010000157         6673 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.159, whole genome shotgun sequence.
ACCESSION   DAETPZ010000157 DAETPZ010000000
VERSION     DAETPZ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.159"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..171)
                     /locus_tag="K0W59_003880"
     CDS             complement(<1..171)
                     /locus_tag="K0W59_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBI3024703.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRFSPSQWAAIGVLGS
                     LLFGLLTYLTEVY"
     gene            complement(321..482)
                     /locus_tag="K0W59_003881"
     CDS             complement(321..482)
                     /locus_tag="K0W59_003881"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024704.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(479..667)
                     /locus_tag="K0W59_003882"
     CDS             complement(479..667)
                     /locus_tag="K0W59_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HBI3024705.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            927..1262
                     /gene="iraM"
                     /locus_tag="K0W59_003883"
     CDS             927..1262
                     /gene="iraM"
                     /locus_tag="K0W59_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HBI3024706.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1543..1674)
                     /locus_tag="K0W59_003884"
     CDS             complement(1543..1674)
                     /locus_tag="K0W59_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HBI3024707.1"
                     /translation="MIAYCFCEWHCSSRIMSVPADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(2174..2250)
                     /locus_tag="K0W59_003885"
     tRNA            complement(2174..2250)
                     /locus_tag="K0W59_003885"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2214..2216),aa:Arg,seq:tct)
     gene            complement(2352..2427)
                     /locus_tag="K0W59_003886"
     tRNA            complement(2352..2427)
                     /locus_tag="K0W59_003886"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2392..2394),aa:Ile,seq:cat)
     gene            complement(2571..3392)
                     /locus_tag="K0W59_003887"
     CDS             complement(2571..3392)
                     /locus_tag="K0W59_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBI3024708.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3407..3763)
                     /locus_tag="K0W59_003888"
     CDS             complement(3407..3763)
                     /locus_tag="K0W59_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBI3024709.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3776..4825)
                     /locus_tag="K0W59_003889"
     CDS             complement(3776..4825)
                     /locus_tag="K0W59_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBI3024710.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRLLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNAQVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRSWVNEKYKRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            complement(4827..5105)
                     /locus_tag="K0W59_003890"
     CDS             complement(4827..5105)
                     /locus_tag="K0W59_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024711.1"
                     /translation="MARNAKYYNHDNSTVLAHTHERYSHAFKSDWYQHPSCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSEQPPRPSRTFQQRIWR"
     gene            complement(5172..5422)
                     /locus_tag="K0W59_003891"
                     /pseudo
     CDS             complement(5172..5422)
                     /locus_tag="K0W59_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5640..5795)
                     /gene="hokD"
                     /locus_tag="K0W59_003892"
     CDS             complement(5640..5795)
                     /gene="hokD"
                     /locus_tag="K0W59_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBI3024712.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(5867..6154)
                     /gene="relE"
                     /locus_tag="K0W59_003893"
     CDS             complement(5867..6154)
                     /gene="relE"
                     /locus_tag="K0W59_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HBI3024713.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            complement(6154..6393)
                     /gene="relB"
                     /locus_tag="K0W59_003894"
     CDS             complement(6154..6393)
                     /gene="relB"
                     /locus_tag="K0W59_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HBI3024714.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            6418..>6673
                     /locus_tag="K0W59_003895"
     CDS             6418..>6673
                     /locus_tag="K0W59_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024715.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSM"
BASE COUNT         1771 a         1784 c         1401 g         1717 t
ORIGIN      
        1 gtacacctca gtcagatatg tcagcagccc aaacagcaga ctccccagca cgcctattgc
       61 cgcccactga gacggggaaa acctgtccag caactgcagg aaccagtagc ccgttcccac
      121 cgctgacgtg gtgtatgaca cacctgttgt gattttttcc atctggtcca taccccgtct
      181 cccgttatcc ggaagctgac aacaataaaa aagccaccag ttaactactg atggctctga
      241 taactcatgc aagcgtctca gacgacccac tgacactacc ggtgagttta acgatacctt
      301 ccatttgact ggctcacttt ttatgatgat gccggtgcat ttatctccag caccagactt
      361 tctatctcaa cgccatacgt tgcatttttg gtaatatccg tcagcgtcag tgcatttagt
      421 cccactgcca gactgtcttt tatggcctgg aatgccgggc cagtacgatg acgtagtatc
      481 actccggctc agttgcacca ctgaccacca catcaccttc tgctgcaatc gcctgcatca
      541 gggtataagg ggttatggcc accggactac caaacggctg ccagccctct ttcagtttat
      601 gtgtcagctt ttccgcaaga tctgacggcg gcgccgccct gacaacatca tagtgtttaa
      661 atgccatggt tctttccacc atctgaaaaa taattcttta aaatacctga catgtaatac
      721 agaaaaaaca caaaaccata ccttaaataa aaacctgatt atcaagcaga tatgcatgga
      781 taaactacaa gacgagatat aaaccaccct gcatttaaat aaacaataaa caacatcaga
      841 aaaataattc tgctctatgg tttaattcaa aaatatcatt tatacttttc agaacatcac
      901 cagcaaggca taaacaagga aactaaatga agtggattgt gattgataca gttatccagc
      961 catcatgcgg aatatctttt tcagtcatat ggagtaaaat aaaattaata atctggtatc
     1021 aatcggatgc tttcttacct cctgaaagta tatttacact gactcacaca ggcatcatgc
     1081 tcaataacaa agtgctacct gtaaccattt acaacgtagt accattcaat aaaacattct
     1141 ggaatttaat caaaaacagc caggaatgcc ctacaaatac agataacgta ttgaatgaat
     1201 gctttaataa ccgttgcact ctgcaaatat gtccttatgg actaaaacaa caaagtccat
     1261 aaggagttta ctcacatctg acaaaatcaa tataaacagc ccctccggag aggggctgga
     1321 gagtggcgct atgtgccatt gcatggtgcc gggtgcctcc cggtgaattc agtaccagca
     1381 cctgaatccg cgattatccc atatacctac tcgctgattg cccctccgca cagggggatt
     1441 caccatgcca gtttctttta acaaactccc cgcaaaccag acaacagtca accgcctgaa
     1501 ttgtgaagta tttaaaaatt tctcccgcta actgataccc ggctaacagt ctggcgtttt
     1561 ctttttcagc aacgggaaag caacaaccac cacacccgcc accagcacac cgtcagccgg
     1621 cactgacatt atccggctgc tgcaatgcca ttcacaaaaa cagtaagcaa tcacttttta
     1681 ccgtaacagg tgataatcca gatatgtatc taccccagat gagtaatccg aagttcatcc
     1741 ataccacagg tcctggctat tctgttgtac tcctgaacaa gagcaaataa ttctgaatta
     1801 gcaaccatga actcatcgca aaccctctgt atagcatcac tattcagaat aataacgtct
     1861 cttcccgaaa gacgatcagg agtacagaac aaaactgtca aacggctgaa ggcctttgct
     1921 cgtgctgcat tgactatatc aatacgctgc ctaaggatga aacaccccga cgcctcatca
     1981 atattcactc tacccacacc atatgaatga taaatactta atgctgaaaa aaccattaga
     2041 ccgtataaca aatactcaat caatacttaa cagaactttt atttttgaca aatatataat
     2101 attttcaaca atatcctgag ccaggtatat ttcagtataa ggctctgccg gaaggaatct
     2161 ggaagaatga atatggcgcg ctgtactgga ttcgaaccag tgaccgattg cttagaaggc
     2221 aattgctctg tccggctgag ctaacaacgc tgaataccga taatggaccg ccatcgggga
     2281 cccgcccccc gcaccaacaa ccctgttatc gtgtcgtctg ctcttcctga taagctaatg
     2341 gcggtttgtg atggtggccc ttgctggatt tgaaccagcg acctggcgat tatgagtcgc
     2401 tcgctctcac cactgagcta aagggccggg agcagaataa taacggtccg taattaattt
     2461 cgcaataaaa aacccgctca gcggcgggtt gtagaaactc ttctaacgtc aggcataaaa
     2521 cgcccatcat tatgacgaat ttaccacaga ttccggaaaa atcaaccttg ttacctagtt
     2581 acctttttta actgccgctc agcccatgct tcttcaatat caaaccgggt caccagcgca
     2641 tcataaaatt tcttaactgt tttttcccat gacgcgcgtg ttatctggtt tgtcacctcg
     2701 catatagcat taaatgcctc cgttgatggt agtctttcat agccacgacc accacaacgc
     2761 tggcagtctc tgataacagg cataccacgt tttaccgact cttcacgatg aatggcgaca
     2821 ccacgcccac ggcaatcctt acaggcggtg gaaacctcac cctttccgcc acactccgga
     2881 caggcaactt ttaccacctc cctgactttt ttccattctt cccagtaaga cggatacaca
     2941 cctttcgtgc actttgccca taccggcggc ttaccatccg gatactggat cttgtttgta
     3001 aaaactatgc tttcaataaa tttttcccca tagcaacaag ggcactgctt tttactcgct
     3061 gcgctgcggg cataatcctc aaaagcgtac gaagccataa tgcgcatcac taccggtttt
     3121 atttctgccg gaagttttct caacgccgcc acacgatcgc accgactgag tgcataatct
     3181 gccagtaatt ctgttgcccg cgccctgtca ttcatactga tgcccatttt ccccaggaac
     3241 gcagaaaaac ccatctcagc ccgattctgt gtcatgccct gcgcggccat cacatcagtg
     3301 atactcagcg catcttttga cgttgaggcc gatgcatcgg tcaggccagg ggattttggg
     3361 gagtagtatt tcggtaaatc ttccagtttc attttttgac ctgctcttaa tgcattatgg
     3421 ggtaaatctt cacccccaga cgtccaccag atactggctg accacgaacg atattgattt
     3481 catcaaactg ctcatcgtcc attaacactc ccgcatgcgt cagcgcatcc agcggtgctt
     3541 tcaggatatt gtccaggtcg cgacgacgct tatccggtgg ctctgcaatc acctttatcg
     3601 ccagccttcc ggacaggctt aatttcagcc gctgctggcg aacaataagc gccacagccc
     3661 ggcgataacg ctttccctcc tccgagataa aatatgtgct gccacggcgt cgccagtaag
     3721 tgtttaccgt cggcgggtaa ggtaaaacca aatctatgag catcagtcac ctcttttacc
     3781 caagcacgcc agttgcaaag gcgtgatcaa gaaaacgaaa aattaaatca acctgagaac
     3841 catgcttttc ttcgaacgcc agaggatccg catgaagctc gttgtgatgc tcccgacaca
     3901 gcggtagcgt gaaaatatcg tgagattttg tccccattcc gccctgacca tgaccaatca
     3961 ggtgatgggg atcgtcggct ggcttaccac aacacgcaca cggctgtgtc ttcacccagc
     4021 gtttgtattt ctcgttaacc cagctgcgac gtttaggtcg tttcatgaaa gattccggag
     4081 actcaggatc aacggcaatg ctgaccaccg tcttttcctg ttgtgggttc tgttgctggt
     4141 gggcgtgagg cagcggcgca agattttttg tgcgctgttt cagtatgctg gtggcggtct
     4201 gctctcccgg tacgatgtcg ctttcacggt acattgagcg gattttttcc gcacgcaacc
     4261 ccagcgaacg acgtaatacc gcttccggta gcgcgtccgc cacctgattg cggaccgccc
     4321 accaggataa ttcagctaga gataattcac gctcctgcgt accgcttatt gcgtgaccga
     4381 tgacgtcaat catccatgct gacaggtttt gatgagcaag ttgctcgagt gattcggatg
     4441 tctggtcacg caactggttg tcgcagtgcc agcacaacac cattgcgccg gtaccataac
     4501 ggtgaatgac ggtttcgctg tgatgataat cgccgtgtgg ccactggcag gatttaatat
     4561 ggcgcaacag ccagtcagac aatgcaccag caccaccagc agcacgaatc acctgtgcgt
     4621 tactgaaaaa cggcagcaat gttttgtctt ccaccagcgg ctggcgaacg gcaggaacga
     4681 ccccggacgg cagattacgc atgcttttcg gttccggctc caccagtaac cgggtattgt
     4741 ggaataccgg catggattca cgaccgggct taaggaccac cagcccgagt tccggaacca
     4801 gaacaggtcg aagtaatacc cgcacgttac ctccagatcc gttgctggaa tgtgcgggac
     4861 ggacgcggtg gctgttcgga gtaagggagc ctgacggaga ttatccagtg acggtagtcg
     4921 aggctaaggg cttttttaac ctcgcatccg cgcctgcggt aacactgaat gagccattcg
     4981 gcctgttctt cagtgcatga gggatgctgg taccagtctg acttaaatgc gtgagaatac
     5041 cgctcgtgcg tgtgggcaag aacggtcgaa ttatcatgat tgtaatattt tgcgttgcgt
     5101 gccatcggtt ttctccggtg gcacggtgtt actcagcggg agttcagccc cgcgcaagat
     5161 tgtagatgag tttattcttc tgaaaaagca gaaaagccag cttttattcc ggtctctttc
     5221 aatgcctgta atgaagtgac aaactcaccg tcgcgcaaga taaatccgtc cgtcactcgg
     5281 gcatccacaa aattaattaa cgcagcccca ttttttgcaa acacacaatg cggtaatgac
     5341 taacaatatt tccattttca acgcacacag catagaggcc atcttcacaa aaaattttac
     5401 gcagttcttc gatgttcatc atcagaatcc ttccagataa gtagcactcc cctgttcggg
     5461 gtccatccct tttatccctg cgcgctactt aagtattttt gattctattc cggcaccatc
     5521 cagaacttca aatgcgttga aaataaaaac aaaaacccgc cgaagcgggt taagtgcggg
     5581 tgcgttgagg atgcctgcca catcagaggt ggcgagggat ttctcactcg ccgggtctct
     5641 tactcctcag gttcgtaagc tgtgaagaca gcgacctccg tctggccggt tcggattcgt
     5701 acctcgcaga ggtctttcct cgttaccagt gccgtcacta tgacggttaa acagatgacg
     5761 atcagggcga ttaacatcgc cttttgctgc ttcatagcct gcttctcctg tcaacgcaaa
     5821 gcagaagtgt caccttcggt gcgaaacaga gatgtcatgc tttggttcag agaatgcgtt
     5881 tgaccgcctc gctatatact tccgagcgtt ctcttttccc aacagaaatc acgaaaacga
     5941 caactttctc gtctataacc tggtatacaa ggcgatagcc tgaagaccgg agcttaatct
     6001 tgtaacaatc aggcatacca cggagcttgt ttgcttcaat ccggggtgac tcaagtactt
     6061 caaccagctt ctttttcaac tgttcacgta ccgtcgagcc cagctttcgc cattccttta
     6121 gtgcccgctc gtcaaaatcc agaaaatacg ccatcagagt tcatccagcg tcacacgtac
     6181 tggcttagga ttacgaagcc gttctttcac tatctccaca agttcagcat cttcatcact
     6241 caggagtgtc tgtttgaacg gcaagcgttc attgtcagcg atatactcga gcatgagacg
     6301 aagcgcttca gaaggagtta cacccatttt ttcaagcgcg gcgtaagaac gcgctttaag
     6361 ttcatcgtca atacgcaggt taatgctacc catgtcttac acctcttgta attacaaatg
     6421 tcattacaag tatcgcacta caacatgctt agggcaagtc acgaaggaag tcagaaagta
     6481 gtcgtaagaa cggtgatcac tgtccgcttt gtgccaggag cagccattgc taagtccatc
     6541 ctgtattgtg caggtcagct cttttttaaa gagtccggcc atcatcttac tggtacagac
     6601 accatatact ttgtgacggt caggctacat atgcacaact caacttattc atctattttt
     6661 tgctttagca tgt
//
LOCUS       DAETPZ010000158         6307 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.160, whole genome shotgun sequence.
ACCESSION   DAETPZ010000158 DAETPZ010000000
VERSION     DAETPZ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.160"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            73..378
                     /locus_tag="K0W59_003896"
     CDS             73..378
                     /locus_tag="K0W59_003896"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3024716.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /locus_tag="K0W59_003897"
     CDS             complement(454..918)
                     /locus_tag="K0W59_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBI3024717.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="K0W59_003898"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="K0W59_003898"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HBI3024718.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEATASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="K0W59_003899"
     CDS             complement(1787..2476)
                     /locus_tag="K0W59_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBI3024719.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="K0W59_003900"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="K0W59_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HBI3024720.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTHKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="K0W59_003901"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="K0W59_003901"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBI3024721.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="K0W59_003902"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="K0W59_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HBI3024722.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1604 a         1705 c         1562 g         1436 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 gttgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgtgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttca gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatggtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaattacct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
     6241 atataacatc attgttttca atctgccatt tatgggattg accgttttct tttgacacgg
     6301 agttcaa
//
LOCUS       DAETPZ010000159         6304 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.161, whole genome shotgun sequence.
ACCESSION   DAETPZ010000159 DAETPZ010000000
VERSION     DAETPZ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.161"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..177)
                     /locus_tag="K0W59_003903"
                     /pseudo
     CDS             complement(1..177)
                     /locus_tag="K0W59_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(172..1098)
                     /gene="rspA"
                     /locus_tag="K0W59_003904"
                     /pseudo
     CDS             complement(172..1098)
                     /gene="rspA"
                     /locus_tag="K0W59_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
     gene            1140..1481
                     /locus_tag="K0W59_003905"
     CDS             1140..1481
                     /locus_tag="K0W59_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HBI3024723.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            1547..2076
                     /gene="speG"
                     /locus_tag="K0W59_003906"
                     /pseudo
     CDS             1547..2076
                     /gene="speG"
                     /locus_tag="K0W59_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2079..2789)
                     /locus_tag="K0W59_003907"
     CDS             complement(2079..2789)
                     /locus_tag="K0W59_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HBI3024724.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            2897..3202
                     /locus_tag="K0W59_003908"
     CDS             2897..3202
                     /locus_tag="K0W59_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HBI3024725.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            3401..3724
                     /locus_tag="K0W59_003909"
                     /pseudo
     CDS             3401..3724
                     /locus_tag="K0W59_003909"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4280..4879
                     /locus_tag="K0W59_003910"
                     /pseudo
     CDS             4280..4879
                     /locus_tag="K0W59_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(5472..>6304)
                     /locus_tag="K0W59_003911"
     CDS             complement(5472..>6304)
                     /locus_tag="K0W59_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024726.1"
                     /translation="LKDAQDNIPFPVEVLPTRNSLPTPIIAHTLSDRSVVVPVRGGER
                     PEVVTVPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTHGTLQQDAMNICENVVRTI
                     NARMVDVLESSKLLKTVELPVLTGSLTTKADAIMDALFENTESSFGSEVSDYGIIAHE
                     SQLKALSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDG
                     ENITVVLSRDGDSQSHDLEILGKVFVVAEAATTVKLDTGAATAVLPVVKRLKFTKTEA
                     "
BASE COUNT         1615 a         1595 c         1549 g         1545 t
ORIGIN      
        1 ttcaggctta ctggcgatcg ggttttctga cgggggactg gtgtcaggtg aatctgtgac
       61 tgacgatgca tcttccagaa gatttctggc tgtgttcagc cggttttcca gttccgacag
      121 tcgtttttct gcctgtcgga tctgattttc aagcttatga cgctttttct ctgaactaaa
      181 atcggcaatc cggcgcatac cggtaattcc gcctgcatgg gtcagcgtgg tgcggatata
      241 atcgatgagt tgctcttcaa tcagttgttt gcagtcccag atgctgttga agacttcacc
      301 cactgcgatg ggtgtgacgg tatgttggcg aatgagacgg aagcattcct ggttttccgc
      361 tggcgtcggg tcttccatcc agaacatgcg ataatcttca atgcttttac caaagcgcgc
      421 cgcttcaata ggcgttaagc gatggtgcat gtcatgcagc aaatgttcat taaaaccaaa
      481 cttgttacgt accgcgtcaa acaatttcgg catgaaatcg aggtatttct ccgtcgacca
      541 cagctgctct tccggccact gtcctttggt tgcgggttca taagccagac ctttaccttt
      601 cgacatgccg taggtggttt tcataccagg gattccgcac tgcacgcgga tggctttgaa
      661 tcccagctcc tgatgacggg cataatcatc cagagcttca tcaatactgt gaccggtggt
      721 atggcaataa accatcaccc cttcacgaga cgcgccgccg agtaactggt aaagcggcat
      781 gttggcagct ttggctttaa tatcccacag cgccatatca accgctgaaa tggccgacat
      841 cgtaaccgga ccgcgacgcc agtaagcacc tttatagaaa aactgccaga tatcttcgat
      901 acggtgcgca tcgcgaccaa taagccgcgg acaaagatga tcctgcaaat aagaggccac
      961 ggaaagctca cgtccattga gggtggcatc cccaaggccc gtaataccgt cctcagtggt
     1021 gatttttaat gtgacgaaat tacgccccgg acaggtaaca aaaacttcag cctggtacgt
     1081 tttgcgttcg cagtggatgg tgtagaatca cctttaatca ttcatacagg gaatgaatta
     1141 tgaaaatcac tctcagcaaa cgaatcggcc tgctcgcttt tctgctgcct tgcgcactgg
     1201 cattgagcac aactgttcat gccgaaacta acaaactggt gattgagtct ggcgacagtg
     1261 cacaaagccg ccagcacgcc gctatggaaa aagagcaatg gaatgacacg cgcaatctgc
     1321 gccagaaagt gaataaacgc actgaaaaag agtgggataa agccgacgcc gcttttgata
     1381 accgcgataa atgtgagcaa agcgccaaca tcaatgccta ctgggagccc aatactttgc
     1441 gctgcctgga ccgtcgaact ggccgcgtta ttacccccta acctgttatt gatttaagga
     1501 atgtaaggac acgttatgcc aagcgcccac agttaagcta cgcccgctgg agcgtgaaga
     1561 tttacgctat gtacatcaac tcgacaataa cgccagtgtg atgcgttact ggtttgagga
     1621 accctacgaa gcctttgttg aactctctga tctgtatgat aagcgcatat tcacgatcag
     1681 agcgaacggc gctttgtggt ggaatgtgac ggcgaaaaag ccggtctggt ggagctggtg
     1741 gaaattaacc atgttcatcg ccgggcagaa tttcagataa ttatctcccc ggagtatcag
     1801 gggaaaggtc tggcaacccg tgccgccaaa ttagcaatgg actatggctt caccgttctc
     1861 aatctctata agctgtatct gatcgtcgat aaagagaatg aaaaagcgat tcacatttac
     1921 cgcaagcttg gctttacggt tgaaggtgaa ttgatgcatg agttttttat taatggtcaa
     1981 tatcgtaatg ccattcgcat gtgtatattc cagcatcagt atctggcaga gcacaaaaca
     2041 ccgggtcaga ctctcctgaa gccgaccgca caatagcatt aataataatc gatcgtattt
     2101 ttgatggtgt aaacccgttc gacggcgggt tttactcctt catcaacaat gatcagctga
     2161 cagtccaccg gattggcgtg actgtcatat tcacagctct gttttacatt accaactcgt
     2221 tgattattta gtaaagtaac cgctgtgtaa tctaattttt tgatcggatc cgttgatggc
     2281 gtggcgctga cagataatgt tttgtcgtta cttttcgtgg ttttacccag cggatacccc
     2341 tgatcatcat agcgatattc catctgcatt tgtttgctgc tggctttaat cacgaagcca
     2401 ttatcatcgg tttcccaact caccccggca gaaggtaatt ctgctagctg gcattttccc
     2461 tgtaaacgta ctctcttctc cagcgtctcg gcatcacggt aataattggc gtccagtacc
     2521 agagcgacca cggtattatt ttccagatcc agtaattcga gtgaatcaaa acagccttct
     2581 tccgacaaag tcccagaaac acgtttcgtc acttcacctt gctcatccat taatgtctga
     2641 gtgaaatctt ttaccggacc acgcagcgga tcaaaatcga attcattaga gaaactggcc
     2701 atctcagggg taaatgaaag tgttacctct gtgcggtcac atcctgtgag gaatatcgcg
     2761 agtaagcatg gtagtaattt gtatttcaca acagtcacca gagagtagag atgattctca
     2821 atcatagtag caaatacagt actttacacg ttaaatgcta tgcttaaaga agttatcttc
     2881 gcgtaaggag cttatgatga aactctcaac ctgctgtgcc gcgcttctgc tcgccctcgc
     2941 ctcacccgcg gtactcgccg cgccgggatc ctgtgagcgc atacagagcg atatatcaca
     3001 gcgcattatc aataatggcg tacccgaaag cagcttcacg ttaagtattg tacccaatga
     3061 ccaggttgat cagcctgatt cccaggtcgt cgggcattgc gctaatgata cgcataaaat
     3121 tctctacaac cgcaccacca gcggtaacgt ctctgctccc gcgcagtcta cccaggatgg
     3181 cgcgcctgcc gaaccgcagt aacacattat cgactgaacg ccggatatga cacatccggc
     3241 gatttgaaca tacaacataa tctcacctta ttactcatac ccttctattg atgtggatta
     3301 ataattctta acccaaaatg ggtagactcc ctctattgtt agcgcgctaa atattcaata
     3361 tataaacatt tatataacga taaagaacag ggagtgagtt atgtccaaaa atgaccgaat
     3421 ggtgggcatc agccgcagaa cactcgttaa atcaaccgcg ataggttctc tggcgctggc
     3481 tgcaggcggt ttttctttac cgttcaccct gcgcagtgca gcggcagcgg tacaacaggc
     3541 cagcgaaaaa gtgatctggg gttcctgttc cgtcaactgt ggtagccgct gtgcacttcg
     3601 tctacatgtt aaagataatg aagtgacctg ggtggaaact gacaataccg gcagcgatga
     3661 gtacggcaac catcaggtac gcgcctgttt gcgcggtcgc tccatccgcc ggcgtattaa
     3721 tcatcccgat cgcttgaatt acccaatgaa acgcgtgggt aaacgcggtg aaggcaaatt
     3781 cgaacggatt agctgggatg aagccctgga tactatcgcc agtagcctga aaaaaaccgt
     3841 cgaacagtat ggcaatgaag ttgtatatat tcagtactct tcggggattg ttggcggcaa
     3901 tatgacccgc tcttcgccat cagcctcggc ggtcaaacgc ctgatgaact gctacggcgg
     3961 ttcactcaat cagtatggct cctacagcac cgcgcaaatt tcctgtgcca tgccctacac
     4021 ctacggcagt aatgatggca acagcaccac ggatattgaa aacagcaagc tggtcgtgat
     4081 gtttggtaac aacccggcag aaacccgcat gagcggtggt ggcatcactt atcttcttga
     4141 aaaagcgcgc gagaaatcga acgccaaaat gattgttatc gatccgcgat ataccgatac
     4201 ggctgccggt cgtgaagacg aatggctccc tattcgcccg ggcaccgatg ccgcgctggt
     4261 tgcgggtatt gcctgggtat tgattaacga aaatctcgtt gatcaacctt ttctcgataa
     4321 atactgcgtc ggctatgacg aaaaaacctt acccgcagat gcacccaaaa atggtcacta
     4381 taaagcctat attcttggtg aaggtgacga taaaacagca aaaacgccgc agtgggcttc
     4441 gcaaattacc ggtatcccgg tagaccgtat catcaaactg gcgcgtgaaa ttggcacaac
     4501 aaaacccgct tatatctgct agggctgggg gccgcaacgc caggcaaacg gcgagctgac
     4561 tgcacgcgct attgctatgt tacctatttt gacgggcaat gtcggcatca gcggcggaaa
     4621 tagtggcgcg cgtgaatcga cctataccat tacgatagaa cgcctaccgg tgctggataa
     4681 tccggtcaaa acgtcaatct cctgcttcag ctggacggat gctatcgatc atggcccgca
     4741 aatgacggca ctccgcgacg gtgtccgggg caaagataaa ctggatgtgc ccattaagtt
     4801 catctggaac tacaaggact tgaagcggtt actcgttaga gtcacgagag accttagtag
     4861 gcctgaggtc ctgcattgag tcaagacctt gtgtcattct ttgtgccaga cttgtgtcat
     4921 tagacgggat gtctagacgg ctggtcagga cccccggtgg cctctttgtc aggactcagg
     4981 gtggcaccgg ggaacattgg tgtcttaatt gtattgaatg aggtctctaa cttttggtcc
     5041 aaaactcatc gttagctgct cgccagctca cagccgaacc cgaactgtaa gcacgaccac
     5101 cagcaccagc ttcagcagga agacgacaag atagacttta tgcaggtcca tgcgccctcc
     5161 tgtgtctatt gtaccactgc actagataac tagtacaaaa taaagtccag accgaaggcc
     5221 cagaccatag ttaattaatc acattgataa tccaccacct gaagtcctgc caacattcag
     5281 tccagcctta cccatgctag gagtcttaac cggggtgtcg ttctgtgtct ccaccactgg
     5341 tgccgcatca ggctctacag gaagctccac agcaggcgca gggaccggag gtgatacgga
     5401 tgtaccacca ccagcggaga agcccttagc gatggaccgg aggcctttac taatgctctt
     5461 acccattgct gttacgcttc ggtcttggtg aacttcaggc gtttgactac aggcaggact
     5521 gcggttgcag cgccagtgtc cagcttaact gtcgtagcag cttcagcgac aacgaagacc
     5581 ttaccgagga tttccaagtc gtgggactga gagtcgccat cacgtgaaag gacgaccgta
     5641 atattctcac cgtcgtgacg gaagcagccg aagctcaggg ccgtgaagcg tttagccagc
     5701 atgaacacac ctttgtcctc accgttgtaa tacgcgatgt cggtacccag catatccacg
     5761 atagcgtctt caccagagaa gccctgttta gcagccagac gggacagcgc tttcagttgg
     5821 gactcatggg cgatgatgcc gtaatcagac acttcagagc cgaaagagga ctcggtgttc
     5881 tcaaacagag cgtccatgat agcgtctgcc ttagtggtca ggcttccagt caggactggc
     5941 agctctacgg tcttcagcag cttggaggac tccagaacgt caaccatgcg agcgttgatg
     6001 gtacggacca cattctcaca gatgttcata gcgtcctgct ggagtgtgcc atgagtgaaa
     6061 ccagtggagg acttggcgtc ccacagttta gtttgctccg agatgaggat tgcctgttcg
     6121 ataggttcga cgacaatctc ctgaccagac ggaacggtca cgacttcagg tcgctcacca
     6181 ccgcgtacag ggacgaccac agagcggtca gacagggtgt gggcgatgat tggggtaggc
     6241 aggctattgc gggtaggcag gacttctaca gggaacggaa tgttatcctg agcgtcttta
     6301 agta
//
LOCUS       DAETPZ010000160         6253 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.162, whole genome shotgun sequence.
ACCESSION   DAETPZ010000160 DAETPZ010000000
VERSION     DAETPZ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.162"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..205
                     /locus_tag="K0W59_003912"
     CDS             <1..205
                     /locus_tag="K0W59_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI3024727.1"
                     /translation="YDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQ
                     RRHSRLGNISPAAFREKYHQMAA"
     gene            563..925
                     /locus_tag="K0W59_003913"
     CDS             563..925
                     /locus_tag="K0W59_003913"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol glucosyl transferase"
                     /protein_id="HBI3024728.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            922..1839
                     /locus_tag="K0W59_003914"
     CDS             922..1839
                     /locus_tag="K0W59_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBI3024729.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRTERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTIVFGNPVRGYPSMLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPRYLIKSRK"
     gene            1852..3117
                     /locus_tag="K0W59_003915"
     CDS             1852..3117
                     /locus_tag="K0W59_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HBI3024730.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAHDLFFIVLSGLSGPFIIFIIASSVFKYLHLSKGVISIRGLYLFYTRLPYLAMILCG
                     LIQATSIILTFNGTRSHAPLGFSFDVMSSIISSNVFLFSFVPWNIANAGWDNHLLSYT
                     LSTLVITLCVFIYIKGNWQMKVFATLPILIVAFSMAKPQLADSVPQLPTLATGNGSRY
                     FVNIHIAIFSLICVCLFQCIKNKTLKIFFKIYVSVILIAMIKLNFFITPLPDMNWSQG
                     AELINKAKHGEAVSFTVLPPWLTLDLIKK"
     gene            complement(3184..3807)
                     /locus_tag="K0W59_003916"
     CDS             complement(3184..3807)
                     /locus_tag="K0W59_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3024731.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSERY
                     THTNIQVTSSGGGKVIVLLNGTTFQSAGDYQPSADRPYWITGNNCELIDIGCTFMEIT
                     SKATSVSAESVTRSGRINANGSVDIAPGVSSVNAHATGVYDVTFSHPLAAATNGYVVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLL"
     gene            complement(4558..4896)
                     /locus_tag="K0W59_003917"
                     /pseudo
     CDS             complement(4558..4896)
                     /locus_tag="K0W59_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
     gene            5018..5278
                     /locus_tag="K0W59_003918"
     CDS             5018..5278
                     /locus_tag="K0W59_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HBI3024732.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQCAKMNNSSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSN"
     gene            complement(5296..>6253)
                     /locus_tag="K0W59_003919"
     CDS             complement(5296..>6253)
                     /locus_tag="K0W59_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024733.1"
                     /translation="RRLVGDALHKNSGPYKDADRAALSRLYGSLADDQKAALSETNAL
                     RDFEVAQRLVQMRKSMEEQMINLTGRTLNGDVSRKATTALQAMSKGDAKGFRELMQNT
                     PSRKLRTELLGTGLRDMLSNGKRGADFNPAGFADWYQNMLANGQMRNLARHLPKETMS
                     GLNDVYKVAKAIKDAKSYEITTGRLNEFVKRFNRVTAANEFVANHAQRIGTAVGSTVS
                     GPFSAVGAVAGSEIGAKVASKIRAMGGAESIESAEKLISSPEFQKAARLAVKQAPESI
                     VDTTVRRSSAWRSFYNSLPESDKKTISRLGIMYWMNSDDNQK"
BASE COUNT         1771 a         1343 c         1245 g         1894 t
ORIGIN      
        1 ttacgacaac gctccgatgg aaagcttctg gggaacgctg aaaaatgaga gcctgagcca
       61 ctatcgcttt aataaccggg atgaagccat ctcagtaata cgggaataca ttgagatttt
      121 ctacaatcgt cagcgtcgtc actctcgtct ggggaatatc tccccggcag ccttcaggga
      181 aaaatatcat cagatggctg cttaaaaaaa gaacaaatgg tagtgtccgc tattgccagt
      241 acacctcaaa cctacgacct tcgcattacg aatctgtagc accaatcata actatctgtt
      301 ttagcaagca ttaaccgcat tcactaagat atagttgatg gcacaaacag aaagttgatg
      361 aatgatgttg ccatatatat gtcacaaata cggcacaacg atcttcaaac atatagccac
      421 acattcacag aagagcacaa agccttgcaa tccagtgcaa agatttgtgt gccagagttt
      481 ttctcatcaa ctaccgcaag tatcgatcga ttgagacttg gatgatagac ttcatgcctt
      541 tgattattag ctgatagaag aaatgttaaa gctatttgca aagtacacat ctataggcgt
      601 gctgaacaca cttatacact gggtggtttt tggtgtatgt atctatgccg cgcataccaa
      661 tcaggctatg gcaaacttcg caggtttcgt tgtggctgtg agttttagct tctttgcgaa
      721 tgcaaaattc acattcaaag catcaactac aacgatgcgc tacatgttat atgtcgggtt
      781 catgggaaca ctgagtgcta ctgttggatg ggttgctgat agatgttcac ttcctccaat
      841 tgtcactctt gttaccttct ccgccatcag cttggtgtgc ggtttcgtct attcaaagtt
      901 cattgtcttt agggatgcga aatgaagata tctcttgtcg ttcctgtctt caatgaagaa
      961 gaagcgatac caatttttta taaaacggta cgtgaattcg aagagttgaa gccatatgaa
     1021 gtagaaattg ttttcataaa tgacggaagc aaagacgcta cggagtcaat cattaacgct
     1081 ctggctgttt ctgatccgct agttgttccg ctgtcattta cacgcaactt tggtaaagaa
     1141 ccagcattgt ttgcagggtt agaccataca accggcgatg ccgtgattcc aattgatgtt
     1201 gacctgcaag acccgattga ggttattcct catcttattg aaaagtggca ggcaggtgct
     1261 gacatggtgc ttgctaaacg ttcagaccgc tcaactgatg gacgactgaa acgtaagaca
     1321 gctgagtggt tctataaatt acacaacaaa ataagcaccc caaaaataga ggaaaatgtc
     1381 ggagattttc gactcatgtc tcgtgaggtt gtggagaaca ttaaactatt gcctgagcgc
     1441 aatcttttca tgaaaggcat actgagctgg gttggtggtc agacagatgt cgttgaatat
     1501 gtacgcacag agcgtgttgc tggcatctca aaatttaatg gctggaaatt atggaatctg
     1561 gcactggaag gtatcacaag tttttcaacc tttcctcttc gcgtatggac ttatataggc
     1621 ttgtttgttg caagcatttc atttttatat ggtgcatgga tgattataga caccattgtc
     1681 tttggtaacc cagtacgcgg gtatccctcc atgcttgtat caatactttt cctgggtgga
     1741 gtgcaactga tcgggattgg tgttctcggt gaatatatag gtagaattta tttagaaaca
     1801 aaatcaagac cacggtactt aattaaaagc agaaaataac aggatgatct tatgaagata
     1861 ttcataatgc gcaattggca caagatatca atttttatac ttgcatttac tttgatttgg
     1921 cttaggcgca ttgacatttt gacaaatgcc cagttttggg ctgaggatgc agttttttgg
     1981 tataaagatg catacgaaca aggttttttc agttcactca taaccccaag aaatggttat
     2041 tttcaaacag tatcaacact cattgttggt gcgacgacat ttattaatcc aatttacgca
     2101 ccattattat caaacttttt tggaataata attagagcta taattatatg gttcttattt
     2161 accgatagat ttaaattcct aagcaccaca tcaaaaatct tcatctctgt atatttaatt
     2221 tgcatgcccg ggcttgatga agtccaagcc aatataacaa atgctcactg gtatttatca
     2281 ttgtatgttg caatgataat aatatctgaa gaatccaagt caaaattatg gaaagcacat
     2341 gatttatttt ttatagtatt atctggattg agcggtccat ttattatatt cataattgca
     2401 tcatctgttt ttaaatattt acacttatca aaaggagtaa taagcataag aggtttatac
     2461 ttattttata ctcgactgcc atatttggca atgatcttgt gtgggctgat acaggcaaca
     2521 tcaataattc ttacatttaa tggaacaaga tcacatgctc cattaggatt cagttttgat
     2581 gttatgtcat ctataatatc atctaatgta tttctttttt catttgtccc ttggaatatc
     2641 gctaatgctg gatgggataa tcacctttta tcgtacacac tatcaacttt agtaattaca
     2701 ttatgtgttt tcatttatat taaaggaaat tggcaaatga aagtgtttgc cacccttcca
     2761 atacttattg tcgcattttc tatggcaaaa cctcaactgg cagattctgt tcctcaactg
     2821 ccaacacttg caaccggaaa cgggtcaagg tattttgtaa atattcatat tgcaattttt
     2881 tcattaatat gcgtttgttt gtttcaatgc ataaagaata aaaccttaaa aatattcttt
     2941 aaaatctatg tttcagtcat ccttattgca atgataaaat taaacttttt tataacccct
     3001 cttcctgaca tgaactggtc tcaaggagca gaattaataa ataaagcaaa acatggagaa
     3061 gccgtctcat ttacagtgct ccctccatgg ttaactcttg atttaataaa aaaataaaac
     3121 ttcacattaa taaataacaa tgaatccgcc tccctacgta ttcccgtagg gaggctgtcg
     3181 tgtttatagt agcctggtaa tgctaaatgc aaacgaactg gagattcctg ctccgctaag
     3241 cgtattgcgc agggtcaccc taaatgtatc tacaccaata taagtaacat cacaactaac
     3301 tgaatcaggc gcagatatag gtgttatctg aaccacataa ccattagtag ctgctgcgag
     3361 cggatgagaa aacgttacat catacacacc agtagcatgc gcgttaacgg atgaaacacc
     3421 cggcgcaatg tctacactac cattggcgtt aattctccct gagcgcgtaa ctgactctgc
     3481 agaaaccgat gtggccttac tggttatttc cataaaggtg caaccaatat cgatcaattc
     3541 gcaattgttc cctgttatcc agtatggtcg atctgcactt ggctgataat cccctgcgct
     3601 ctggaacgtt gtgccattga gtaagactat aacttttccc ccacctgagc ttgttacttg
     3661 tatattcgtg tgcgtgtagc gttcactgct cacgcgatga aaatttccac cgtttataac
     3721 taccgttaca ggtcgcgtcc cagtattatc tatagcaagg tctgcccccc cggcattagc
     3781 ctcgaagtat gggttgttta acaccaaagc acacgaaccg ttaaggccat ttatggctaa
     3841 ttgcgcacca cctgtaccgg aatccccctg agtaccattt ccttcacaag tcaggctgtt
     3901 gatagtagtt cctgcccccc atctatcacc aagtataccc catcgtgtat tctggcttgc
     3961 agttaatcgg ttaactgtaa tggcattaag ctctgaccct tcactggagt taagtgcgcg
     4021 ccagccaata cggttaccct gtaagtacac attatctaac tctgacgtta acaccgagtc
     4081 tgtacgcaac ccttcgccgg tagtgtgccc ataagacaca atgttctcta atttagtgta
     4141 tgctttattg gttacgagaa gaccgtaaga cgatcccgaa cctttaagtg taatgtcccc
     4201 cagataatca taggcgtgta cacgttgggt tgccccgact gtatcaccga gcatctttaa
     4261 gttgaaatcc gaggcatggc agttaaatat cgtttctgcc atattttctc cggaataatg
     4321 agcacggact cctgtcgaac caataaattt agcgtcatag ttgccgtaat caagaacgag
     4381 tggtgacgcg atatcgtaca ccccttgctt tatttttaat gttccaccga aaggccacga
     4441 tgtaagcggg tcttgatata tttcattttt tattgcaaat agctcctctt ggagattttt
     4501 gccgctatta gtgccaatgt gagcagctcc ttcatctgat tgaagtaact gctttaactg
     4561 atcaggatcg tacttcagca cattctgaaa atagaactgc tgcgaaccat acgcatcata
     4621 tacagccatt gaatggcctt gctcagttac aaatttggca atctgcccgt tgtacacagg
     4681 atatccggca gcattgataa caattggctg agaaacaggg acgtgagatc cgtcttcatt
     4741 ctctacataa acctgaatct ggttttcagg atttaccggg tcagtgtcaa tttttccgat
     4801 ataaattttg ccattggata cggctttaaa agaacgcgcc atagtgaaga gttgcgaagg
     4861 catgctgacc acaacatttg cggcgatatc tgacatttca ttgctccaga cgaatgatat
     4921 gatgcaatca tgatgtgatt gcataccgaa atggcactat tgaatattta tccagtaggt
     4981 tacgatgcca ttccacccaa ctggtgaggc atcaaggatg tacagcaaat acgacgaggc
     5041 acagtttcac ttgagacttc cgcatgaact ccacgcgaaa attaaacagt gtgcgaagat
     5101 gaataacagt tcgctgaact cagagataat tgcagcgatt gaagaatcat tggctaaaca
     5161 aagctctgca tcagtttaca ttgacgatgc agagcgtatg gcagaacaac aatctgatat
     5221 ggttaagaaa attgtctttg atacgctcaa ggagctatat aaaaaagaca gcaactaacc
     5281 atccgtggct ttccgttact tctggttatc atcactgttc atccagtaca tgatgcctag
     5341 ccttgatatg gttttcttat ctgattctgg aagtgagttg taaaacgagc gccaagcaga
     5401 agagcgtctt acagttgtat caacgatgct ttctggtgct tgttttactg ccagccttgc
     5461 tgctttctgg aattctggtg agctaattag cttttctgca gattcaattg attcagcgcc
     5521 gcccatcgcc ctgattttgc tggcgacttt tgccccaatt tctgacccag caacagcacc
     5581 tactgcactg aacggtcctg acacagttga accaaccgca gtgccaatgc gttgggcatg
     5641 gttagcaaca aattcatttg ccgcagtgac gcgattaaac cgtttgacga actcgtttag
     5701 tcttcctgta gttatctcgt aagattttgc atctttgata gcctttgcga ccttatatac
     5761 atcgttcaga cctgacatag tctcttttgg taaatgtctg gcaagattgc gcatctgccc
     5821 gtttgctaac atgttttgat accagtcagc aaaccctgca ggattaaaat cagcgccacg
     5881 ttttccgttc gaaagcatat cccgaagacc tgttcccagt agctcggttc ttagcttcct
     5941 ggacggcgtg ttttgcatca attcacgaaa tcctttggca tcgcctttcg acattgcctg
     6001 tagtgctgta gttgctttgc gagaaacatc accgttcagc gttctgccag ttagattaat
     6061 catttgctct tccatgcttt ttcgcatctg aacaagcctc tgagcaactt caaaatcacg
     6121 taatgcattt gtctctgaca gcgccgcctt ttgatcatcg gcgagcgaac cgtaaagcct
     6181 cgataaagca gcgcgatcag catctttata tggtccagaa ttcttatgaa gtgcatcacc
     6241 aacaagtcgt cgt
//
LOCUS       DAETPZ010000161         6172 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.163, whole genome shotgun sequence.
ACCESSION   DAETPZ010000161 DAETPZ010000000
VERSION     DAETPZ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.163"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..173
                     /locus_tag="K0W59_003920"
                     /pseudo
     CDS             1..173
                     /locus_tag="K0W59_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            750..1878
                     /gene="ltrA"
                     /locus_tag="K0W59_003921"
                     /pseudo
     CDS             750..1878
                     /gene="ltrA"
                     /locus_tag="K0W59_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            1961..2140
                     /locus_tag="K0W59_003922"
                     /pseudo
     CDS             1961..2140
                     /locus_tag="K0W59_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2170..2652)
                     /locus_tag="K0W59_003923"
                     /pseudo
     CDS             complement(2170..2652)
                     /locus_tag="K0W59_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2908..3888)
                     /locus_tag="K0W59_003924"
     CDS             complement(2908..3888)
                     /locus_tag="K0W59_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBI3024734.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3888..5087)
                     /locus_tag="K0W59_003925"
     CDS             complement(3888..5087)
                     /locus_tag="K0W59_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI3024735.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5409..5747)
                     /locus_tag="K0W59_003926"
                     /pseudo
     CDS             complement(5409..5747)
                     /locus_tag="K0W59_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5814..6172)
                     /gene="istB"
                     /locus_tag="K0W59_003927"
                     /pseudo
     CDS             complement(5814..6172)
                     /gene="istB"
                     /locus_tag="K0W59_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
BASE COUNT         1668 a         1421 c         1474 g         1609 t
ORIGIN      
        1 ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta
       61 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
      121 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa attgcagcat
      181 aagttatcca tgctctgagg tgggtggagg tgggccctcc ggtcagggtt tacaggagca
      241 ctgaccaaac gacccggtgc tgcttcattc taaggaatga ggtggtgagc gactcgggaa
      301 aaggcagaga tctactgtca cgtggggttc gggaagctga acgaaagtaa actgtccgat
      361 gaagcatcga aatcttagat gatgccaaaa tggggccgta cagccgtccc ctgataagcg
      421 cagaagacac ctgcttactg actgcgcggc atccggtgtg aaggcagcag gatcccgggc
      481 cagaccatct cagggaacgt gagaacctgt ttccggcacg tatcgggaaa caggggcgga
      541 cccattcgta gtagtgatga aagatctgtc atgggtctgg agcgaaggga tggggatcac
      601 attcattgca gaagacacaa cccaaaaggg gatgacgtaa ctgatgcaac gaaaatcatt
      661 tgaaatacca aaagcactgg tctgggcatc atatctggat gtgcgtcgca acaagggagc
      721 cccccggatg cgacgggcag actttgaaaa tgttcgacca gcaacgtgat ggcaatctat
      781 acaagatctg gaaccggcta tgttcgggaa catggtttcc gccaccagtg ctggaaaaac
      841 ggatcccgaa gtcgaatggc aaggaacgca ttctggggat cccaacagta tccgatcgaa
      901 ttgctcaggg agcgataaaa cttttcatgg aagaaaagct tgacccgatt ttccatgctg
      961 attcatatgg ttaccgacca ggcaaatcag ctcatgacgc tctgaaacaa tgtgccatcc
     1021 ggtgctggcg ttatagctgg atactggaag tggacatcag tgcgtttttc gaccatgtga
     1081 gacatgatct ggtgctcaaa gctctggaac accacgggat gcctaaatgg gtcatcctgt
     1141 actgtcgacg ctggatggaa gcgccaatgc agagttgtga aaatggagaa ttaataaccc
     1201 gaacacgagg cactccgcag ggcggggtca ttagtccgct acttgctaac ttgttccttc
     1261 actatgcatt tgatttgtgg atggaaagag aatatcgggg ggtaccgttt gagaggtacg
     1321 ctgacgatat tgtagtacat tgttcacgaa tgagtgatgt aacaaggcta aagaacaggt
     1381 tatcggagcg cttctcggaa gtcgggctgg tcctgaatgc agggaaaacg aatatcgcct
     1441 acattgacac gtttaaaagg agaaacgtcg caacgagttt cacctttctc ggatatgact
     1501 tcaaagtgcg tacgctgaag aatttcaaag gcgaacggta ccgaaaatgc atgccgggtg
     1561 cgtcaaatgc agcaatgctg caaaataaca gaaacaatca agaagtggcg tatacatcgc
     1621 tcaacagctg agagtttgct ggattttgcg aggcgctaca atgcgatagt gagaggctgg
     1681 atcgagtact acggaaagtt ctggtccaga aatttcaact atcgactgtg gagtgcaatg
     1741 cagtcacgtc tgctcaagtg gatgcagtct aaatacagac tttcgaaccg gaaggctcag
     1801 cgaaagctga cgctggtaag gaaggagtat ccgaagctat ttgttcactg gtatttacta
     1861 cgtgcatcaa atgagtgatc aagagccgta tgacgcgaga gtgtcacgta cggttccgtg
     1921 agcagccggg gagtgaaatt ctctccggct gactcgactc gcgggttccg acagcgatga
     1981 cgaacatgcg gcggtgttgt actcgctgat cggcacatgc cgtctgaaca atgtggaacc
     2041 agaaaaatgg ctgcgttacg tcattgagca tatccaggac tggccggcaa accgggtacg
     2101 tgatctgttg ccctggaaag ttgatctgag ctctcagtaa atatcaatac ggttctgacg
     2161 agccgcttac ttttggcgta attctgctgc gcctttactg aacacaatgc cgtattcagc
     2221 aagaagtcca cgaatgtgat ttatcagttg tgtcctttcc attgaaacca accgacgcat
     2281 attcaaaaaa gtctggacgt cctgttgctc cagagttttt attggtacgg gtcgtatagt
     2341 tccatgttgg caggcctcag ctattgcctg tgcgtcgtta taatcatttt tctggcaccg
     2401 ctgataagct ttaacatgtt gtgccggaag caatattaca tcatggtcga gtttcctgat
     2461 ttcccgggcc cagtaatgag atgccccgcc agcctccatc gcaacagtca ttttaggctg
     2521 ttgcacctgc cagttaagca gttgattgcg agaaagtttg gtctttcctg cgggtttgcc
     2581 atgagcattg atactgaaaa gataaaagtt cgttttagcc agatcaatgc cggtgatttt
     2641 ctgttcagac atggtatccc gcttactttt gtggttaaag tttatccatc atggcgcaat
     2701 tgaaggctgc cgtgaaagag ggggaagact atctcatcgg ttactgttaa acggctaaga
     2761 gaaactatct ttttcaagca aagaagatac ggtgaaacag gcgatttcac cgtgaaatac
     2821 tgttcaaaaa aaacgctaat aaaagcaaaa tttcttttaa aacagccaat taaaaactgg
     2881 tgagttgctg gcgaaaaact tgaattttca cagcgaattc agattatctt ttagtacaag
     2941 agcaatcata cgatctaact tatcctgaac ttccaacggt aatctgccga attcatatga
     3001 gaacgtacgt ccctttactc gttttcgagc aaatttatct ttactatcaa atttccataa
     3061 caaagttaca accgctttat cctttactcc tgaatcaagc aatgaagaca tttccttggt
     3121 tataagccgc ataatactgt ttttaacctc gtcttcagaa agattgtcat cagcagtaat
     3181 aagctcaata ctcggcgaga tattcttcac aacttcatca atagatatat cacccagcct
     3241 cagtcgttca cttaattcag ccagttgcct gtaatcggcg tatgtcagct cagactgaac
     3301 aggaaaaaga gacacaaaat ctttaggtac ggacgctgct tggagggcac gagtcacctt
     3361 agctgccgac aatccctctc tttccgcaat ttgcttctgg ttcataccgg cctctttgag
     3421 acgtactaaa cgcaacccaa tttcacgaat attatgttca agtgacgtct gcaaatcttt
     3481 cgccagatgc tgagcttcag aaacagtaag ctcatcatcc gttacaagta cacgtaatcc
     3541 aaccttgcat agcaaagctg ctgcccgacg acgggaacca tccaaaatct caataagatc
     3601 gccagtccga atcccaatac atggatagaa ttgttgtaaa cgtatagttc tggtgatgtc
     3661 ttttagtgag tccggcgtca gggctgtctg ttcgcggcca ttcacctctt caaccacaaa
     3721 agtttgagtg tccactttgt catgatcaac cttgatctca gtaaattttg cctttcggcc
     3781 agtattgagg gtaaatacat gaactggagc cgatatttct tcagtattat tcaggatatt
     3841 tgtattcagc gtacggccaa tagtcggtcg gtgctttctg ttttccatca gtagttggcc
     3901 cttataaatt caatacgatc aaaaactgct ttagcgaaat cttctgctgc catacgtgca
     3961 tttttcaaag cttcaccact gccaacataa gtaacaggat tcgcagatat cacggtatca
     4021 aacgactcgc cgcttctctc aaaaccatca agacgtggca ttgaaacatc aagcatgtca
     4081 ccaccgaaaa tctcttttgt caggctatgg cagtatttat gatctgactt attcgccagt
     4141 ttcgacatga agccaatatt tgcctgcaaa cggcatgaac atccatcctg ttcaataatc
     4201 tgtacaagtt caggtaaacg agccaaatat ttaagcgtgg aatggaaatc aacttgtgct
     4261 ggcggcaccg gtgtaaacat gatatctgcg gcagcaatgg catttttcaa aaatgcgtcc
     4321 agatgcgggc ctgtatcaat aaggatgaag tcgaaatcat gcttcagctt atcaataata
     4381 ttctcacgca atatcgcgtg tttgttttgt cctaatagat gctcctcgca cagagtatcc
     4441 cagtttgatg caataaaggc gtcgtcaata gaagccggca taacataaac gccagggata
     4501 actgacggaa cgataaaatc ttccagtaat tcctcacgac tcacgttctg caacatagcc
     4561 tgcggtgcgg ttgtatcaac cagccccacc gcatgcaagt agttcagaaa catagtggct
     4621 gatgactggg gatctaaatc aaggagcaga atacgtaaat cttctgacag caggtgagga
     4681 tgagcacgca gggcgtgagc tacggaaaca ctactcacgg ttttagacac accgcctttt
     4741 agactaccga tgaatatgga atatgcctca ctataacgat cgcgatattt aggaatgcca
     4801 cgatgtgcat agatatcgat aatgttctgg atggttaatg catattttgt cgcggttcct
     4861 gctgggcgtt tttcaaactg atagccctga gcctccatct caccgactgc accatccacg
     4921 cttgcgcgcg ttaattttgg aagcttagct acagcagact ttgaatagac ttgataaaaa
     4981 acatcagcct gaaactcctg cttttgctcc tgaatctgtt ttgttagggc gagcaacatt
     5041 ttatctgcac gctgcgcaac cttacttaac tgctcaaaac tagtcattcc ccctccgtgc
     5101 tatgtaggat tttaacttat tacaaaaaaa actgcattag cagggtaatg catgcaagta
     5161 catattgtac atgcttatat taaaaatgcg cgcattgctg atttattaat catcggaatg
     5221 aatattactg taagatagta tgcctttccg ggagaaagac gctgatttcc cccgaataat
     5281 ccagttcacc accacactcc ggacaacagc tttcttcagg ctccagatgg tgtatttcgc
     5341 gggaaacatg ttccggtaac ggacggcgat gacgtgactg tcgcaacagg cgggggtagt
     5401 gcttccaatc agtagaacat gtgcttttca atgaaggagc cgatgacttt ttcgtggatt
     5461 tccacggaac gcgagaagca gattgtttta cgtgccagcc gcttgatgcg ggtgcggagg
     5521 gtccggttgt tacgttcaat acgttgcgtg aagattttgc cggtcaggtg tttctccttc
     5581 ggcacttctc tggcgtagct gccccagtca tcgctggtca ccatgccgat acagaacgga
     5641 gtcaacaggg ctaacagttc ccgacaggtc tcatcattcc gtggcccgaa agtgtaagcc
     5701 aacacaccgc cagttttagt gttgtacgca taccagagcc agtgttgtgt caacgacgga
     5761 tgaaaagtgc tccacttata tctccaccaa cggcccaata ttgatccacc gttttactca
     5821 ggattagctt ctgctataac cccggccttt cgtttctgtc tgagtcgata gctttctcct
     5881 ttgatttgaa cgacatgtga gtggtgtaag atacggccca gcatcgctga ggtcagtgct
     5941 gcatcaccgg cgaacgtttg atcccactgc ccgaacggta gattggatgt caggatcatt
     6001 gcgctctttt cgtaacgttt agcgatgacc tggaagaaca gttttgcttc ttcctgactg
     6061 aacggcagat agcctatttc atcaatgatg agcaggcggg ggccattact ccacgctgaa
     6121 gcgtcgtttt ataacggccc tgacgttgtg ccgtagataa ctgaagtaac ag
//
LOCUS       DAETPZ010000162         6153 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.164, whole genome shotgun sequence.
ACCESSION   DAETPZ010000162 DAETPZ010000000
VERSION     DAETPZ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.164"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            368..2020
                     /locus_tag="K0W59_003928"
     CDS             368..2020
                     /locus_tag="K0W59_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBI3024736.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2031..2201)
                     /locus_tag="K0W59_003929"
                     /pseudo
     CDS             complement(2031..2201)
                     /locus_tag="K0W59_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2334..2453)
                     /locus_tag="K0W59_003930"
                     /pseudo
     CDS             complement(2334..2453)
                     /locus_tag="K0W59_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2527..2961)
                     /locus_tag="K0W59_003931"
     CDS             complement(2527..2961)
                     /locus_tag="K0W59_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HBI3024737.1"
                     /translation="MDTFKRRNVATSFSFLGYDFKVRTLKNFKGELYRKCMPGASNAA
                     MCKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFSYRLWSAMQSR
                     LLKWMQSKYRLSNRKAQRKLALVRKQYPKLFAHWYLLRASNE"
     gene            complement(3138..4447)
                     /gene="ltrA"
                     /locus_tag="K0W59_003932"
                     /pseudo
     CDS             complement(3138..4447)
                     /gene="ltrA"
                     /locus_tag="K0W59_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019332377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(4511..4711)
                     /locus_tag="K0W59_003933"
                     /pseudo
     CDS             complement(4511..4711)
                     /locus_tag="K0W59_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            5089..5838
                     /locus_tag="K0W59_003934"
     CDS             5089..5838
                     /locus_tag="K0W59_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBI3024738.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(6008..>6153)
                     /locus_tag="K0W59_003935"
     CDS             complement(6008..>6153)
                     /locus_tag="K0W59_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3024739.1"
                     /translation="NRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQR
                     LRN"
BASE COUNT         1641 a         1445 c         1384 g         1683 t
ORIGIN      
        1 atcggaaacg atgatctggc agcctgagtt cacagataaa acattctcca ggaaacttgg
       61 ggcggttcac gtcagtatca gaccatatta attgctgaaa ctgataaaca tcaaacctta
      121 tagaagggtg aatgctgaaa gggggctatg gtgatcgacg tgatgacaac gacaggtaag
      181 accgtgacct gataaacctg aattgtgtcc tagacataaa gtccgtgaag ggaataaggc
      241 tcaggtcagc gaatcttata gtggcccgca gctctgactg caataaggcc ggatacagag
      301 cagcaactac tcgtcgatgt cagaacaacg gaagccttgc aaacggggat gcgttcatac
      361 aggtacaatg ataccgttaa aaaaaaacat tacgctaata atgttcaccc tgtcattact
      421 gacaggaaat cctgctatag cgtatgaaac agataaagta tataaaataa ccgttctgca
      481 tacaaatgac caccatggac acttttggcg taacaatcat ggtgagtacg ggttatcctc
      541 ccaaaaaact ctggtggaca atatccggca gaaagtaata aataatggcg gaagtgttct
      601 attattatct ggtggtgata ttaacaccgg tgtacctgaa tcagaccttc agaaggctga
      661 acccgatatt cgtggaatga atctaattgg ttatgatgca atggccgtcg gtaatcatga
      721 atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat ttcctttcct
      781 ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat ggaaaatttt
      841 catccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata ctgcgaaaac
      901 aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg aggcgaggag
      961 tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg caacacatat
     1021 gggtcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg aaatggctcg
     1081 cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag ctcctgtctg
     1141 catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct gtgtaccaga
     1201 taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg tcggtcaggc
     1261 tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac acccagttaa
     1321 tctcaaaatg agaattacca gagaagatgg aaaaacggaa ttttcttttt acacccccga
     1381 aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata aaggcaaagc
     1441 acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca gaagtaaagt
     1501 tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag aacgtattga
     1561 cgcagatttt gctgttgtca gtggtgggga aattcgagat tccatagaga gtggtaacat
     1621 cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa gtattgattt
     1681 gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac cggattccgg
     1741 agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa tcgaaatgct
     1801 aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa catttagctt
     1861 taatgctact ggtggggatg gctatccacg tattgataat agacccggat acataaatac
     1921 cggatttatt gatgctgaag tacttatcga atatatccgg aaacattcac cgctggatgc
     1981 tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt gacgatcatc
     2041 acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg gatgtttgcg
     2101 atatgggaat gcagttgctg tattttgtgt ttctgcttct gccagttgtt gctgaattta
     2161 accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt cgctgattaa
     2221 agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccatat tgatatttac
     2281 taagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc cggtcagtcc
     2341 tggatatgct caatgacgta acgcagccat ttttctggct ccacattgtt cagacggcat
     2401 gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctatcgga accccgccgg
     2461 agagagtttc actctccggc tgctcacgga accgtacgtg acactctcgc gtcatacggc
     2521 tcttgatcac tcattcgatg cacgtagtaa ataccagtgg gcaaatagct tcggatactg
     2581 tttccttacc agcgccagct ttcgctgagc cttccggttc gaaagtctgt atttagactg
     2641 catccactta agcaaacgtg actgcattgc actccacagt cgatagctga agtttctgga
     2701 ccagaacttt ccatagtact caatccagcc tctcactatc gcattgtagc gcctcgcaaa
     2761 atccagcaaa ctttcagctg ttgagcgatg tatacgccac ttcttgattg tttctgttat
     2821 tttgcacatt gctgcatttg atgcacccgg catacatttt ctgtacaatt cgcctttgaa
     2881 attcttcaga gtacgcactt tgaagtcata tccgagaaag gagaaactcg tagcgacgtt
     2941 tctcctttta aacgtgtcaa tgtaggcgat attcgtcttc cctgcgttca ggaccaaccc
     3001 gacttccgag aagcgcaccg ataacctgtt cttcagcctt gttgcatcac tcattcgtga
     3061 acaatgtact acaagtcgag tcacccgagg gaacctcccc ctctggtgct cacggaaccg
     3121 gacgtgaacc tctcagctca tccggctctt ctcattgagg cctgtcccca tagtaacggc
     3181 cagtgtgcaa ataacctcgt ttcactccgc gcaacacggg ccagccagtg gctggcccgt
     3241 cgtcggtggc tcctaagtcg cttgtgttta cggggcagcc ataacacgag tttgcgatcg
     3301 atttgcctca gcgtcagata cagtgctgat ttataaaaag caccgtagta ctttatccag
     3361 cctctgattt tcgcattgaa catatgcgcc agctcgtaca aggacttgtc actgcgattc
     3421 tcctcacctc ctgacggatt gcttttcctg ctctggcact catcgccgga ctgaagttaa
     3481 cgaaggtctt accccagcga ttcatcgaca gacgcggccg gaatgtatag cccagaaagt
     3541 tgaagctcgt ttccgggtag tccccccgtc ggtcagcatc cttacagtaa actattcgtg
     3601 ttttttgcgg atgcagctgc agcccgcatt gcgcaagccg ctgcctcagt ccacttatca
     3661 gagcgtcagc ctgccactgg ctgtgacagt ggcatacaac atcatcggcg tacctttcga
     3721 agggaacgcc gggaaactga cgttgcatcc acatatcaaa ggcatagtgc aggaacagat
     3781 ttgagagcag gggggagatt acgccgccct gcggcgttcc tcgaccccgt gccagcattg
     3841 ctccatccgg catttgcacc ggtgccttca gcctgcgctc aatgtacatc accacccagc
     3901 gtgcctgagt atgatgctgc actgtcttca gtagcaggtc atgatcgata tcatcaaaga
     3961 agcctttaat atcgacttcc accacccagt caaacttcca gcagcgtttc ctcgcctgcg
     4021 caatcgcctg gtgtgaggat ttacccggtc gataaccata cgagtctgca tggaacaggg
     4081 gttccagtac cggctcgagt atctgcttaa cgaccatctg ggcgatccgg tccgacacgg
     4141 ccggaatacc gagcggacgt acaccaccgt cagctttggg aatgtccaca cgccttaccg
     4201 cctgcggcat ataactgccg gaagccattc tgttccacag tttgtagaga ttgtctgtaa
     4261 cattctcgtc aaacccggcc agtgtctgcc cgtcaatgcc tgccgcccca cggttggctt
     4321 ttactcgttt ataagcttcc catacctgtc gtttacttac aggaaatggc tttgccgatt
     4381 ttacagcagt cctcccatcg gcattgtcgc ccgtggttgc cgccgcacca tcagcacaat
     4441 ggggcaagcc ctttgcttcg gacccattac ggtccttcac cgctactacg gtttactccg
     4501 tccctgtacc atttttcagc gttccccaga agctttccat cggagcgttg tcgtaacagt
     4561 tacctttacg cgacattgat gttttcagac caaactgctc ctgtatgacc cggtaatcgt
     4621 atgcgcagta ctgtgaacct cgatcggagt ggtggattag cccggcaggt gggcgctggc
     4681 tcctgagcac cataaacagg gctttacccg taaatattgg ctccttctga tgccattcta
     4741 tttcaggaag gagtgtccgt taaactcagg ctacctcaaa aagttatcgg tgcgtttatc
     4801 gaaaaacata tgttctacta attggagtca ttgtctgtaa aagcaactca aaaatacaag
     4861 ttgcttattc cccttgttgg taagaactta attctaactg tttgttctgg atgttttaaa
     4921 cgtcctcctt tttatgcttg ttcataacgg atatttaata ggtaaccttt gttttagcat
     4981 cttctgattt atttaaatta tgtgatagcg cacacaattt tattatccag tactttatac
     5041 tcttaataaa ataattataa atatttatcc tctataagga gatgttatat gaaaagacag
     5101 ctttttactc ttagtattgt cggggtattt tctttaaata cctttgcatc attccctccg
     5161 ggaaatgatg tgacaacaaa acctgacctt tactacctga caaacgataa tgctattgac
     5221 agtctggcat tattaccgcc tccaccacaa atcggaagta ttgccttcct gaatgaccag
     5281 gccatgtatg aaaaggggcg tttattgcga aatactgaaa gagggaagct ggcagctgag
     5341 gatgccaatc tcagtagtgg tggcgtggca aatgtatttt ctgctgcttt tggttctccg
     5401 ataacggcta aagattcacc agaattgcat aaattactga caaatatgat tgaggatgcc
     5461 ggagacctgg caacgcgttc agcaaaagaa tattatatgc gtattcgacc ttttgctttt
     5521 tatggtgttt ctacgtgtaa tacaaaagaa caggatacat tatccagaaa tggctcttat
     5581 ccatcaggtc atacatcgat tggttgggca acagcacttg tattgtccga aataaatccg
     5641 gcacgccagg ataccattct gaaacggggt tatgaactgg gggacagcag ggttatttgc
     5701 ggttatcact ggcaaagtga tgttgacgca gcacgtattg ttggctctgc tattgtagca
     5761 acactgcact caaatcctgt gttccaggca caattacaaa aagcgaaaga tgaattcgct
     5821 aacaatcaga aaaaataatg cttttagcca gtacgcctta taaccccgac cttcagggct
     5881 aatgccagtc agttaagcaa ctgactggct ctttttcagg gctgtggggt atttccaggg
     5941 cctctccttt accactctcg ggaaggccct ttcccttctt gtcggtaatt tcactaggct
     6001 gtgtccctta attacgtaag cgttgataaa agagtcggat gcagcccagt ttcaccatcg
     6061 ccaggtaatt tctcgccgtt ttgtcgtaac gcgtggcgat gcggcgatat tctttcagcc
     6121 gcccaaagca ccgttcaacg acattacgat tgc
//
LOCUS       DAETPZ010000163         5994 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.165, whole genome shotgun sequence.
ACCESSION   DAETPZ010000163 DAETPZ010000000
VERSION     DAETPZ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.165"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..268)
                     /locus_tag="K0W59_003936"
     CDS             complement(<1..268)
                     /locus_tag="K0W59_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBI3024740.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATE"
     gene            805..2706
                     /gene="mtlA"
                     /locus_tag="K0W59_003937"
     CDS             805..2706
                     /gene="mtlA"
                     /locus_tag="K0W59_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBI3024741.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGNVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSFDDSSANLFKLGAENIFLGRKAATKEE
                     AIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKTGVVF
                     CQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDE
                     VLELLAGRK"
     gene            2801..3949
                     /gene="mtlD"
                     /locus_tag="K0W59_003938"
     CDS             2801..3949
                     /gene="mtlD"
                     /locus_tag="K0W59_003938"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBI3024742.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            3949..4536
                     /gene="mtlR"
                     /locus_tag="K0W59_003939"
     CDS             3949..4536
                     /gene="mtlR"
                     /locus_tag="K0W59_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HBI3024743.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(4548..4757)
                     /locus_tag="K0W59_003940"
     CDS             complement(4548..4757)
                     /locus_tag="K0W59_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024744.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5041..5403
                     /locus_tag="K0W59_003941"
     CDS             5041..5403
                     /locus_tag="K0W59_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HBI3024745.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         1565 a         1454 c         1488 g         1487 t
ORIGIN      
        1 gttcagtcgc tttctcctgc gacgcaacat gtgactgcat accccagcca cgctccggtt
       61 gatagagcgt cgcccagatc cacagaaacg gccagataac atgcagagta aacaggctca
      121 cccagcccgc cgtatgaata gcgtcggcat gggggtgatt gcgctttttg gcaatcagat
      181 aagggatgtc atgtatcgcg atgattccat aaaaaatcac cagaaagacg aagatcagca
      241 ctcccaacgc gaaatagttt agaaacatat ctgcctcgga ttcacgttta tcagtgttgt
      301 tttttggctg gcagccagaa gggagtcagg ctgatatttt gacaataatc cgggttcgcg
      361 attctcgcca taacaccaaa gaataatttt tagaggtgat gagttgctta gttacataac
      421 gattgtatga cgaaggcata acatgctgta gatcacatca ggtgaacgcc gtaagaaaat
      481 atcttgtgat tcagatcaca aagattcaac aagccatcaa aacaaaaatg tgacattact
      541 cacatttaaa tgccattttt agcgaaaatc accgccttgt ttctttttta cacaggcgtt
      601 ttgtgatgag cgtcacgtca attacctctc taccccctat atttatgtga ctgatatcac
      661 acaaaagccc gtcgactgga cagttaaccg attcagtgcc agatttcgca gtatctacaa
      721 ggtccggcta cctctgccgc cacattaaca aaaaacctcg ggcttccagc ctgcgcgaca
      781 gcaaacataa gaaggggtgt ttttatgtca tccgatatta agatcaaagt gcaaagcttt
      841 ggtcgtttcc tcagcaacat ggtgatgcca aatatcggcg cgtttatcgc gtggggtatc
      901 atcaccgcgt tatttattcc aacagggtgg ttaccgaacg agacgctggc gaagctggtc
      961 gggccgatga tcacttatct cctaccgctg ctgatcggtt ataccggtgg taagctggta
     1021 ggcggcaaac gtggcggcgt agtcggtgcc attaccacca tgggcgttat cgtcggcgca
     1081 gacatgccga tgttcctcgg ttctatgatt gcaggtccgc tgggcggctg gtgcattaag
     1141 cacttcgacc gctgggtaga cggtaagatc aaatccggtt ttgagatgct ggtgaataac
     1201 ttctccgcag gcatcatcgg gatgatcctc gctattctgg cattcctcgg cattggcccg
     1261 attgttgaag ccctgtccaa aatgctggct gcgggcgtta acttcatggt tgtccatgac
     1321 atgctgccgc tggcgtctat ctttgttgaa ccggcgaaaa tcctgttcct caacaacgcc
     1381 attaaccacg gtatcttctc gccgctgggt attcagcagt cccatgaact gggtaaatca
     1441 atcttcttcc tgattgaagc taacccaggt ccaggtatgg gcgtgctgct ggcgtacatg
     1501 ttctttggtc gtggtagcgc taaacagtct gcgggcggtg cggcaatcat ccacttcctg
     1561 ggtggtatcc acgaaatcta cttcccgtat gtgctgatga atccgcgtct gatcctcgca
     1621 gtcatcctcg gcggtatgac tggcgtgttc acgctgacta tcctgggcgg tggtctggtt
     1681 tctccggcat ctccgggttc tatccttgct gtactggcga tgacaccaaa aggtgcttat
     1741 ttcgctaaca tcgcgggtgt gtgtgcggcg atggctgtct ccttcgttgt ctctgctatt
     1801 ttgctgaaaa ccagcaaagt gaaagaagaa gatgatattg aagcagcaac tcgtcgtatg
     1861 caggacatga aagctgagtc taaaggcaca tctccgctat ctgcgggcaa tgtgactaac
     1921 gacctgagcc acgtacgtaa aatcatcgtt gcctgtgacg ccggtatggg ttcaagtgcg
     1981 atgggcgcag gcgtgctgcg taagaaaatt caggatgcag gtctgtcgca gatttcggtc
     2041 actaacagcg cgatcaacaa cctgccgcca gatgtggacc tcgtcatcac tcaccgtgac
     2101 ctgaccgaac gcgctatgcg ccaggttccg caggcacagc atatttcgct gaccaacttc
     2161 ctcgacagcg gcctgtacac cagcctaacc gaacgtctcg ttgctgccca gcgccatact
     2221 gaaaacgaag tgaaagtgaa agacagcttt gacgattcca gtgctaacct gttcaaactg
     2281 ggcgcggaga acatcttcct cggtcgcaaa gcagcaacca aagaagaagc gattcgtttt
     2341 gctggcgagc agctggtgaa aggcggttac gttgagccgg aatacgttca ggcgatgctg
     2401 gatcgtgaaa aactgacccc gacttatctg ggtgagtcta tcgcggtgcc acacggtacg
     2461 gttgaagcga aagatcgcgt actgaaaacg ggcgtggtgt tctgccagta cccggaaggc
     2521 gtgcgcttcg gtgaagaaga agatgacatt gcccgtctgg tgattggtat tgcagcccgt
     2581 aacaacgagc acattcaggt tatcaccagc ctgaccaatg cactggatga tgagtccgtc
     2641 atcgagcgtc tggcacacac caccagcgtg gatgaagtgc tggaactgct ggcaggtcgt
     2701 aagtaatcca atcctaccct ctcctcacgg agagggtttg ggtgagggaa aagcctcacc
     2761 ccagccctct cgggtaaaaa cattgatgaa ggttaatact atgaaagcat tacattttgg
     2821 cgcaggtaat atcggtcgtg gctttatcgg taaactgctg gcagacgcgg gtatccaact
     2881 gacgtttgcc gatgtcaatc aggtggtact tgatgccctg aatgcccgtc atagctatca
     2941 ggttcatgtg gtcggtgaaa ccgagcaggt agataccgtt tccggcgtca atgctgtcag
     3001 cagcattggt gatgatgtcg ttgatctgat tgctcaggtc gatttagtca ctaccgccgt
     3061 tggcccggtt gtgctggaac gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa
     3121 agaacaaggt aatgaatccc cgctgaacat catcgcctgt gaaaacatgg tacgcggcac
     3181 cacgcagctg aaaggtcatg tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga
     3241 agagcacgtt ggctttgtcg attccgccgt tgaccgcatc gtaccgcctt cggcttcggc
     3301 aactaacgat ccgctggaag tgacggtaga aactttcagc gaatggattg tcgataaaac
     3361 gcagttcaaa ggcgcactgc cgaacatccc aggcatggag ttaaccgaca acctgatggc
     3421 atttgtcgaa cgtaaactct tcaccctgaa cacgggtcat gctataaccg cgtacctcgg
     3481 aaaactggcc ggtcatcaga ccattcgtga cgctattctc gacgagaaaa tccgcgcggt
     3541 ggtaaaaggt gcgatggaag aaagtggtgc ggtactgatc aagcgctacg gctttgacgc
     3601 agacaaacat gcggcgtaca tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa
     3661 agatgatgta gagcgcgtag gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct
     3721 gatcaagcca ctgctcggta cgctggaata cagccttccg cacaagaatc tgattcaggg
     3781 gattgctggt gcaatgcact tccgcagtga agatgatccg caggctcagg aactggcagc
     3841 actgatcgct gacaaaggtc cgcaggcggc gctggcacag atttccggtc ttgatgccaa
     3901 cagcgaggtt gtatccgagg cggtaaccgc ttataaagca atgcaataat ggtggaccag
     3961 gcgcaggaca ccctgcgccc gaataacaga ttgtcagata tgcaggcaac aatggaacaa
     4021 acccaggcct ttgaaaaccg tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc
     4081 tttctgatca ccgccgtcga gcttttgacc gaggcggtaa atcttctggt gcttcaggta
     4141 ttccgcaaag acgattacgc ggtgaagtat gctgtagaac cgttactcga cggcgacggt
     4201 ccgctgggcg atctttctgt gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc
     4261 caggaatacg aagatgcgga actgctgatg gcattgcgtg aagagctaaa tcacgacggc
     4321 aacgagtacg cctttaccga cgacgaaatc cttggaccct ttggtgaact gcactgcgtg
     4381 gcggcgttac caccgccgcc acagtttgaa ccagcagact ccagtttgta tgcaatgcaa
     4441 attcagcgct atcaacaggc tgtgcgatca acaatggtcc tttcactgac tgagctgatt
     4501 tccaaaatca gcttaaaaaa agcctttcaa aagtaagcaa cgtctgctta ctgcccctct
     4561 acctgcttcg gccgataaag ccgacgataa tactccagac gttgtagata taacggcacg
     4621 ctttcatcgg gtattccgga cggaatcgcg ttacggggag gaagtttttt cagatactcc
     4681 cggaacgcct ggcttgatgc catgaaatct acggctttat cgataagaag cggaacgttt
     4741 tcacctaatt tgcccatgat gatatctccg tattaccccg cgccgggaat gcgcggccgc
     4801 caattttagt ttaggttccc ggtaaagatg acattaagga aaacgtgctg aatcctcaat
     4861 aatcatccgc tgctgtttat tatctttttc tatagatttc ttaattaatc aacgaattgc
     4921 gtagtttttg agcatgttaa tgattacgtt gcagaactat tacaatgtca tcagttgtga
     4981 acaaagcacc tggtcgcgca tactaggggc tataaattta tctttatcag aagccatcac
     5041 atgaaagaag tcgaaaaaaa cgaaatcaaa cgtctcagcg atcgcctgga cgccatccgc
     5101 caccagcagg ccgatctgtc gctggttgaa gccgcagaca aatatgccga gctggaaaaa
     5161 gagaaagcca cgctggaagc agaaattgct cgcctgcgtg aggttcatag ccaaaaactg
     5221 agtaaagaag cacagaagct gatgaagatg ccgttccagc gcgcaattac caaaaaagag
     5281 caggctgata tgggcaagct gaagaaaagt gttcgcggac tggtggttgt gcacccaatg
     5341 accgcgctgg ggcgcgaaat gggcctggaa gagatgactg gattttctaa gactacgttt
     5401 taagcccctc agacgactgg ttcgatcgta tcaacccgca tctggcttgc cttagctggt
     5461 caatgcgggg actctcctgt ctactcgcct aattaccaaa caattcactc cctttacttc
     5521 gatttaccgc tggttgatat tctgaaaatg catttttacc gaaatagcca tctataaaca
     5581 ataaaaatat atagaagata tcatttgtta attaacaagc atttacgtct ctttttttcg
     5641 gatttaattg tgaaatttta atcatcatat tcacaaaatc aattatctgt aaaatttttc
     5701 ccagacatgg tttctttcat atttgacatt tattaataat catcattaga atccacgaaa
     5761 aataatatat tcatacaaac acagacatct tttgaaacga atgatacatt tttatgatga
     5821 atgcttaaaa caatatttaa tcgacacaca tcactgtcat taatgtaaag caacatagct
     5881 caccaataat acaatggaca actctaccgg cattgatcgc tggtcggagg aaataaggat
     5941 ttttacatga acttgaagaa gatttttttc agtgctgtca ctgtttcagt tctc
//
LOCUS       DAETPZ010000164         5940 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.166, whole genome shotgun sequence.
ACCESSION   DAETPZ010000164 DAETPZ010000000
VERSION     DAETPZ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.166"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            63..581
                     /gene="mddA"
                     /locus_tag="K0W59_003942"
     CDS             63..581
                     /gene="mddA"
                     /locus_tag="K0W59_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HBI3024746.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            578..1027
                     /locus_tag="K0W59_003943"
     CDS             578..1027
                     /locus_tag="K0W59_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBI3024747.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1028..1260)
                     /locus_tag="K0W59_003944"
                     /pseudo
     CDS             complement(1028..1260)
                     /locus_tag="K0W59_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
     gene            complement(1346..1519)
                     /gene="ortT"
                     /locus_tag="K0W59_003945"
     CDS             complement(1346..1519)
                     /gene="ortT"
                     /locus_tag="K0W59_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HBI3024748.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1714..1809
                     /gene="yncL"
                     /locus_tag="K0W59_003946"
     CDS             1714..1809
                     /gene="yncL"
                     /locus_tag="K0W59_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HBI3024749.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2211..3020)
                     /locus_tag="K0W59_003947"
     CDS             complement(2211..3020)
                     /locus_tag="K0W59_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBI3024750.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3230..4655)
                     /gene="patD"
                     /locus_tag="K0W59_003948"
                     /pseudo
     CDS             complement(3230..4655)
                     /gene="patD"
                     /locus_tag="K0W59_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
     gene            complement(4677..5471)
                     /locus_tag="K0W59_003949"
     CDS             complement(4677..5471)
                     /locus_tag="K0W59_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3024751.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5461..5808)
                     /locus_tag="K0W59_003950"
                     /pseudo
     CDS             complement(5461..5808)
                     /locus_tag="K0W59_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT         1555 a         1506 c         1320 g         1559 t
ORIGIN      
        1 tgcttcgggc tgttttcgat agtattgata acatcatcat cattatttta ttgaggccag
       61 acatgtccat ccgttttgcc cgcaaagccg actgtgctgc cattgcggaa atttataacc
      121 acgccgtgtt gtatacggcg gctatctgga atgaccaaac ggtggatgca gataaccgca
      181 ttgcctggtt tgaagcgcgg accatagcag gttatccggt gctggtaagt gaagaagatg
      241 gcgtagttac gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata
      301 ccgtggaaca ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat
      361 tgttaagccg attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga
      421 tcgaatcgca aaatcaggct tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg
      481 cacaaatgcc gcaggtaggc actaaatttg gtcgttggct ggatctgaca tttatgcagt
      541 tgcaactcga cgagcgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc
      601 ttttttgatt gccgccggaa ttggtctggt agtccagaac acgctgatgg tgcgcatcac
      661 ccagacctcc tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct
      721 ctttgtttct attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag
      781 cgtgcgctgg tggacactta tacctggctt gctgggttcg ttttttgtct tcgccagtat
      841 cagcggatat cagaatgtgg gagcggcgac aactattgcg gtactggtcg ccagccagct
      901 gattggcggt ctggtgctgg atatcttccg cagtcatggc gtaccactac gggcgttgtt
      961 cggcccaata tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg ccagacgctc
     1021 attttaatca gaggatggtg ccgcctttgg ttaactgttc gtggccgctt ccatatcttc
     1081 tttatacttg cgaccgtgat gagcaatagc ggtgcgcaaa cgctgctgct gggtgtagcg
     1141 atcttcccga cttaactccg cgtcatcgct cagggcgatt aataattcgt tcatatgggc
     1201 aatgacgctc tcttcaatcg cggcatccac gcgcgcgatg acttcatcca gatgcgacat
     1261 actttctcct tagccacttg tgaaatttat cgattaatcc aaatgatata agtctgcgtg
     1321 gtgccgtcaa gccagtgagt aagatttaaa acagtgaaaa caacagcgcc accgggaaac
     1381 tcatcggcca tgtggccccc accagaaagg cgcttaagaa acggatgcgt ttcttatcgt
     1441 gagaaagaaa ccaggtgata agaaatgcga ttgctgccat aaccgcataa aaaacaagca
     1501 tatgttgata gagagacatg tacgggcgtc cagccatatg aataaaacgc gcgcaaatat
     1561 gcgccatttt ttgacgtgca tcaagttttt tcaccagttt gagaaaaaat catcaaacag
     1621 caacttgccc ttatccatct tcagaatgag tattattgcg gattttctta acccgtacta
     1681 tacacatagg ggcaatgata aaaggtggca aaaatgaatg tttccagtag aactgtagta
     1741 ctgataaatg tctttgctgc tgttggtttg tttactctta tctctatgag atttggctgg
     1801 tttatttgat gtagtgattt tgcacgcagc acgtactgtc agagaaatca actctctgac
     1861 agtacgcaag atcttctcat actctccaag tttgcaacat tatccttaat atttacttct
     1921 ttttgctggc atctacattg aaatgagtag aaaatatccc cctactgtcg tctatacaaa
     1981 cagcttactg attcaaacgg aatcttgttt tcacattttt ctatcacgaa tactttatac
     2041 gcgcgggagt ctataaatgc tttatacttt caaggaataa cgtaaatata aaaagatggc
     2101 gtaaagtata tgcatggcag gatgtttgca acctccttga tgacgctgcc ccattgaaat
     2161 tgcaaacccc gccctggtta tcttgcccat gtatcatgca aaacagataa ttataggcct
     2221 ctgaatacct cagaaagatt gaatttttta tagtccctct cttgaatatt tccatttgat
     2281 atcaatcgat tcatctcttc aacaaaataa tttatatcat cacacttgtt attatccgga
     2341 agatctgagt agttataatt ccaccatttt attctcaaca gttcatcaat aacatttgaa
     2401 tcaaacctaa atctgattat ttttgcagga actcctgcaa ctatggcata tgggggcaca
     2461 tctttggtaa caacggaatt ggcagcaata acagcaccat cacctattgc gatgccacct
     2521 ttcaatacaa catcatttcc tatccagaca tcatttccta taatcggatt ttcaggtttg
     2581 gtaatgaatg gtttcaatgt ccagctacgt tcaaattcac tcatagcaaa cttatcaaat
     2641 tcaccgttat aagtcatcat gtgagtagta aaacgattca aaggatgatt tccacccatt
     2701 atttttacat ttgatgcaat actacaaaat cgacctatac gtatattccc tgaaaaatgg
     2761 caggtcggaa atgaaaaagc gccgacactg gagaaatttt tcacaggcat tgttgaatat
     2821 tcttcagcaa taacattatc ttcaattgtt atcttgtcac cttttgagaa aagaaataaa
     2881 ttcctttctt tatacgactt cttcccctta tgatttaaaa agattttatt atccatacaa
     2941 aactcaaagt gttttttcgt ccatctgaag gtaagggttt tatttttatc ttccgtgaat
     3001 ttgtacaatt gaactttcat tgttaatact tctacttata aattattagt ttaataacga
     3061 tactctaaag ccttatgcta atcaactgtg acaggatgaa aaatcgccat caaacattta
     3121 gcctaatttt tacgccagtt attaatgtaa tcattctaat acttaaccgc cattaaactc
     3181 agaacctaaa acgatagtgg ccgtcctata acagccacta acatctaact taatgtttaa
     3241 ccatgacgtg gcggacgacg gtgtaatcct ccagcccata aagtgacata tccttgccgt
     3301 aaccagaaag tttctgccca ccgtgcggca tttcacttac cagcatgaaa tgggtattga
     3361 cccaggtaca accatattgc agccgtgcgc tgacgcgatg cgccctgccc acatctttcg
     3421 tccataccga agatgcaagt ccgtactggc tgtcattcgc ccagttcacc acctgttctt
     3481 cgttgtcgaa gggcgtaaca ctcactactg gaccaaatac ctctttttgc acgatggcat
     3541 cgtcctgtaa tgcgccagcc agcagcgtcg gcgcatagta atagccatta cccttgcgct
     3601 tttcaccgcc agtgatcact ttgatgtgcc ctgtcgcttt cgcctcttct actgccttgc
     3661 tgacgcgttc gagatgcgcc agcgagctta aaggtccaag ctccgtagac tcgtcatccg
     3721 gcgcaccaga ttttaacgtt gccaccgcag gcacccagtt tttccaccag cgtatcgtaa
     3781 atgccttttt gcgcgtagat ccgacaagcc gcagtacaat cctgtccagc attgtaatag
     3841 ccaaatgtac gtacaccttc gaccactgct tcaatatccg catcatcaaa aacaatcact
     3901 ggcgctttgc caccaagttc catatgagta cgcttaatgg acgacgcggt atggctgatg
     3961 atgtgctcgc cggtggcgat agagcccgtc agcgacacca tccgcacttt gggatgaccg
     4021 gtcagcggat cacccaccgt tttgcctctg ccaaacagta tgttaatcac gcctgccggg
     4081 aagatatctt tcgccagctc tgccaacttc aacgcggtca gcggggtaat ttctgatggt
     4141 ttaagcacta cgcagttccc tgccgccagc gccggagcaa gtttccacgc ggccatcatc
     4201 agcggataat tccacggtgc gatagaagcc acgaccccca acggatcgcg acggatcatc
     4261 gaagtatgac cttcaaaata ttcacctgcc gccagaccat tcagacagcg cgccgcaccc
     4321 gcgaaaaagc gaaaaacatc gacaatcgcc gggatttcat cattgaacgc actatgcagc
     4381 ggtttgccac aattacggga ttccagttcg gcaaaaacct gaccattttc ttcgataaca
     4441 tccgccagtt tcagcaggca ttccgcacgc gctttcggtg tggtttgccc ccattcagca
     4501 aatgctgcat ctgccgcacg cacagcagca tcgacctgct ctgcggatgc ctcggcaatc
     4561 tccagtaaaa cgtcccccgt tgccggatta tagacaggct gtttttcccc ttcgccgcta
     4621 accagttctc cgttaatcag taacttatgt tgcatagatt attcctgtat cagtggttat
     4681 tgaccattgt cgccttcgcg ggttagccac caggccccca ggatcggcaa ggttgttacc
     4741 aacataacca gcagtgccac cacgttggtt accggtacat cacgcggtcg cccaagctga
     4801 ttgagcaacc acaacggtaa cgttcgttca tgacctgccg taaaggtcgt aacgatgatt
     4861 tcatcgaacg acaaggcaaa cgccagcatt cctcctgcca gtaacgccga actgagattc
     4921 ggcaacacta cgtagcggaa ggtttgccag ccattggccc caagatccat tgacgcctca
     4981 accagactcc acgaggtgcg gcgaaaacgg gcgatgacat tgttaaacac caccactacg
     5041 caaaaagtcg catgaccgac cacgatggtg aaaaatcccg gctccagatt gatggtttta
     5101 aaggcggtta atagcgccag accagtgaca atgcccggca gcgcaatggg cagcagcaat
     5161 aacagcgaaa tggcgttttt gccgaaaaag tctcgtctcc acagcgcggc agctgccagc
     5221 gtccctaaca ccagcgcaat taatgtcgcc agcgccgcca ctttaagtga cagcgtcacg
     5281 gcatcaagaa tatcactacg ctgtgctgcc acgctaaacc agcgcagcgt caggccctgc
     5341 ggtggaaaac taaacgccgc atcttcagtg ttaaacgcat aggcggcgat tatcaggatg
     5401 ggaaaatgta ggaaaacaac gccgccccag gccgccagtt tgaggaaaaa cggtgcgcgt
     5461 tcagagtgca tcgaacgctc ccagacgttt cacgaacgcc aggtacagtg cgatgagaat
     5521 aatcggcacc agggtgaatg ccgccgccat cggcatattg ccaatcgccc cctgctggga
     5581 ataaaccata ttgccgataa aatatcctgg aggaccaacc agctgcggga cgataaaatc
     5641 gcccagtgtg agtgagaagg taaagataga gccagcggca atacccggga ttgccagcgg
     5701 tagcaccaca tagcgaaagg tttgtcgtgg acgtgcgccg agatcagccg acgcctgcaa
     5761 caatgacggc ggcaaacgct caagcgccgc ctgaacgggc aggatcatcg agtaggcagc
     5821 ctgacggctg cggcttgtca tggtctgcaa ttaccgttat aaaaacaggc aatgtcattg
     5881 tctttcaggt ggttatatgg cccgttcagc taaaccccgt aaacgtaaac ccgcctctca
//
LOCUS       DAETPZ010000165         5868 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.167, whole genome shotgun sequence.
ACCESSION   DAETPZ010000165 DAETPZ010000000
VERSION     DAETPZ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.167"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            29..244
                     /locus_tag="K0W59_003951"
     CDS             29..244
                     /locus_tag="K0W59_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBI3024752.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            237..623
                     /locus_tag="K0W59_003952"
     CDS             237..623
                     /locus_tag="K0W59_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HBI3024753.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            631..1317
                     /locus_tag="K0W59_003953"
     CDS             631..1317
                     /locus_tag="K0W59_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBI3024754.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1295..1918)
                     /gene="tetR(B)"
                     /locus_tag="K0W59_003954"
     CDS             complement(1295..1918)
                     /gene="tetR(B)"
                     /locus_tag="K0W59_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HBI3024755.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2000..3205
                     /gene="tet(B)"
                     /locus_tag="K0W59_003955"
     CDS             2000..3205
                     /gene="tet(B)"
                     /locus_tag="K0W59_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HBI3024756.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3318..3911)
                     /gene="tetC"
                     /locus_tag="K0W59_003956"
     CDS             complement(3318..3911)
                     /gene="tetC"
                     /locus_tag="K0W59_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HBI3024757.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4044..4415
                     /locus_tag="K0W59_003957"
     CDS             4044..4415
                     /locus_tag="K0W59_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3024758.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4425..5588)
                     /locus_tag="K0W59_003958"
                     /pseudo
     CDS             complement(4425..5588)
                     /locus_tag="K0W59_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
     gene            complement(5603..>5868)
                     /locus_tag="K0W59_003959"
     CDS             complement(5603..>5868)
                     /locus_tag="K0W59_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3024759.1"
                     /translation="ATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQR
                     IERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         1607 a         1110 c         1282 g         1869 t
ORIGIN      
        1 accactatta agtcatgtag ctggttttat ttcaattgaa cgttttcaaa gtctagctac
       61 agaaggaaaa atgttatcgc tatcttggtg ggaaaacgaa tacgcagttc tgcaatggaa
      121 aaatcatgtt ttacatgcga aagctcaaca agaagggcga gagtcaatat ttgattttta
      181 caaaattagt attgctcata ttactcgcga atattcattt aaaaaggaca aggataatgt
      241 ttgatgttca cgttgtttta gataatcaaa taggacaatt agcattacta ggaaaaacat
      301 taggtaataa aggtattgga ttggaagggg gagggatatt tacggttggt gatgaatgcc
      361 atgctcattt tcttgttgaa caaggaaagg aagctaaaat agcgctagag caagctggac
      421 tgttagtact tgcgatccgg acaccattaa ttcgtaagtt aaaacaggaa aaaccggggg
      481 aacttggcga aatagcacga gtattggcgg agaataacat taatatttta gtgcaataca
      541 gtgaccatgc taaccaactg atattaataa cggacaatga tagtatggct gcatctgtta
      601 cgctcccttg ggcaataaag tgaacttgcg atggctaatt taatacgaaa agaggttacc
      661 tttgagtcct caatagccgc gataggggcg gctatgtctg acatttcacg agttaaaata
      721 ctcagtgctt tgatggatgg gcgagcttgg acggccactg agctaagttc tgtggcgaat
      781 atatcagctt caacggcgag cagtcattta tctaaattat tagattgcca gctaatcaca
      841 gtagtagctc aaggcaagca tcgttatttt cggctagcag gaaaagatat tgctgaattg
      901 atggaaagta tgatggggat ctccttaaac catggcgtac atgccaaagt ttccacgcca
      961 gtgcatttac gaaaagcacg tacttgctat gatcatttag ctggcgaagt tgccgttaag
     1021 atctatgatt ccctttgtca acagcaatgg atcactgaaa atggttcaat gatcacatta
     1081 agtggtattc aatattttca tgaaatggga attgacgttc cttccaaaca ttcacgtaaa
     1141 atctgttgtg cgtgtttaga ttggagtgaa cgccgtttcc atttaggtgg gtacgttgga
     1201 gccgcattat tttcgcttta tgaatctaaa gggtggttaa ctcgacatct tggttaccgt
     1261 gaagttacca tcacggaaaa aggttatgct gcttttaaga cccactttca catttaagtt
     1321 gtttttctaa tccgcatatg atcaattcaa ggccgaataa gaaggctggc tctgcacctt
     1381 ggtgatcaaa taattcgata gcttgtcgta ataatggcgg catactatca gtagtaggtg
     1441 tttccctttc ttctttagcg acttgatgct cttgatcttc caatacgcaa cctaaagtaa
     1501 aatgccccac agcgctgagt gcatataatg cattctctag tgaaaaacct tgttggcata
     1561 aaaaggctaa ttgattttcg agagtttcat actgtttttc tgtaggccgt gtacctaaat
     1621 gtacttttgc tccatcgcga tgacttagta aagcacatct aaaactttta gcgttattac
     1681 gtaaaaaatc ttgccagctt tccccttcta aagggcaaaa gtgagtatgg tgcctatcta
     1741 acatctcaat ggctaaggcg tcgagcaaag cccgcttatt ttttacatgc caatacaatg
     1801 taggctgctc tacacctagc ttctgggcga gtttacgggt tgttaaacct tcgattccga
     1861 cctcattaag cagctctaat gcgctgttaa tcactttact tttatctaat ctagacatca
     1921 ttaattccta atttttgttg acactctatc attgatagag ttattttacc actccctatc
     1981 agtgatagag aaaagtgaaa tgaatagttc gacaaagatc gcattggtaa ttacgttact
     2041 cgatgccatg gggattggcc ttatcatgcc agtcttgcca acgttattac gtgaatttat
     2101 tgcttcggaa gatatcgcta accactttgg cgtattgctt gcactttatg cgttaatgca
     2161 ggttatcttt gctccttggc ttggaaaaat gtctgaccga tttggtcggc gcccagtgct
     2221 gttgttgtca ttaataggcg catcgctgga ttacttattg ctggcttttt caagtgcgct
     2281 ttggatgctg tatttaggcc gtttgctttc agggatcaca ggagctactg gggctgtcgc
     2341 ggcatcggtc attgccgata ccacctcagc ttctcaacgc gtgaagtggt tcggttggtt
     2401 aggggcaagt tttgggcttg gtttaatagc ggggcctatt attggtggtt ttgcaggaga
     2461 gatttcaccg catagtccct tttttatcgc tgcgttgcta aatattgtcg ctttccttgt
     2521 ggttatgttt tggttccgtg aaaccaaaaa tacacgtgat aatacagata ccgaagtagg
     2581 ggttgagacg caatcgaatt cggtatacat cactttattt aaaacgatgc ccattttgtt
     2641 gattatttat ttttcagcgc aattgatagg ccaaattccc gcaacggtgt gggtgctatt
     2701 taccgaaaat cgttttggat ggaatagcat gatggttggc ttttcattag cgggtcttgg
     2761 tcttttacac tcagtattcc aagcctttgt ggcaggaaga atagccacta aatggggcga
     2821 aaaaacggca gtactgctcg gatttattgc agatagtagt gcatttgcct ttttagcgtt
     2881 tatatctgaa ggttggttag ttttccctgt tttaatttta ttggctggtg gtgggatcgc
     2941 tttacctgca ttacagggag tgatgtctat ccaaacaaag agtcatcagc aaggtgcttt
     3001 acagggatta ttggtgagcc ttaccaatgc aaccggtgtt attggcccat tactgtttgc
     3061 tgttatttat aatcattcac taccaatttg ggatggctgg atttggatta ttggtttagc
     3121 gttttactgt attattatcc tgctatcgat gaccttcatg ttaacccctc aagctcaggg
     3181 gagtaaacag gagacaagtg cttagttatt tcgtcaccaa atgatgttat tccgcgaaat
     3241 ataatgaccc tcttgataac ccaagagggc attttttacg ataaagaaga tttagcttca
     3301 aataaaacct atctatttta tttatctttc aagctcaata aaaagccgcg gtaaatagca
     3361 ataaattggc cttttttatc ggcaagctct tttaggtttt tcgcatgtat tgcgatatgc
     3421 ataaaccagc cattgagtaa gtttttaagc acatcatcat cataagcttt aagttggttc
     3481 tcttggatca atttgctgac aatggcgttt accttaccag taatgtattc aaggctaatt
     3541 ttttcaagtt cattccaacc aatgataggc atcacttctt ggatagggat aaggttttta
     3601 ttattatcaa taatataatc aagataatgt tcaaatatac tttctaaggc agaccaacca
     3661 tttgttaaat cagtttttgt tgtgatgtag gcatcaatca taattaattg ctgcttataa
     3721 caggcactga gtaattgttt tttattttta aagtgatgat aaaaggcacc tttggtcacc
     3781 aacgcttttc ccgagatctc atctattgaa acagcttgat agcctttttc aacaaacaat
     3841 attcgtgctg agttaaccag tgattgatag gtactcttaa aattttcttg ttgatgattt
     3901 ttattttcca tgatagattt aaaataacat accgtcagta tgtttatggt atcatgatga
     3961 tgtggtcgtg acaatcttaa gaacatttag gttattttat gtatattgaa cagcattctc
     4021 gctatcaaaa taaagctaat aacatccaat taagatatga tgataagcag tttcatacaa
     4081 cggttatcaa agatgttcta ttatggattg aacataattt agatcagtct ttactgcttg
     4141 atgatgtggc gaataaagcg ggttatacca agtggtattt tcagcggctg ttcaaaaaag
     4201 taacaggggt cacactggct agctatattc gtgctcgtcg tttgacgaaa gcggctgttg
     4261 agttgaggtt gacgaaaaaa actatccttg agatcgcatt aaaatatcaa tttgattccc
     4321 aacaatcttt tacacgtcga tttaagtaca tttttaaggt tacaccaagt tattatcggc
     4381 gtaataaatt atgggaattg gaggcaatgc actgagagat cccctcataa tttccccaaa
     4441 gcgtaaccat gtgtgaataa attttgagct agtagggttg cagccacgag taagtcttcc
     4501 cttgttattg tgtagccaga atgccgcaaa acttccatgc ctaagcgaac tgttgagagt
     4561 acgtttcgat ttctgactgt gttagcctgg aagtgcttgt cccaaccttg tttctgagca
     4621 tgaacgcccg caagccaaca tgttagttga agcatcaggg cgattagcag catgatatca
     4681 aaacgctctg agctgctcgt tcggctatgg cgtaggccta gtccgtaggc aggacttttc
     4741 aagtctcgga aggtttcttc aatctgcatt cgcttcgaat agatattaac aagttgtttg
     4801 ggtgttcgaa tttcaacagg taagttagtt gctagaaccc atggctcctt tgccgacgct
     4861 gagtagattt taggtgacgg gtggtgacaa tgagtccgtg tcgagcgctg attttttcgg
     4921 cctttagagc gagatttata caatagaatt tggcatgaga ttggattgct tttagtcagc
     4981 ctcttatagc ctaaagtctt tgagtgacta gatgacatat catgtaagtt gctgataggt
     5041 ttccagtttt ccgctcctag gtctgcatat tgtacttttc ctcttactcg acttaaccag
     5101 taccaaccca gcttctcaac ggatttatac catggcactt taaagccagc atcactgaca
     5161 atgagcggtg tggtgttact cggtagaatg ctcgcaaggt cggctagaaa ttggtcatga
     5221 gctttctttg aacattgctc tgaaagcggg aacgctttct cataaagagt aacagaacga
     5281 ccgtgtagtg cgactgaagc tcgcaatacc ataagtcgtt tttgctcacg aatatcagac
     5341 cagtcaacaa gtacaatggg catcgtattg cccgaacaga taaagctagc atgccaacgg
     5401 tatacagcga gtcgctcttt gtggaggtga cgattaccta acaatcggtc gattcgtttg
     5461 atgttatgtt ttgttctcgc tttggttggc aggttacggc caagttcggt aagagtgaga
     5521 gttttacagt caagtaatgc gtggcaagcc aacgttaagc tgttgagtcg ttttaagtgt
     5581 aattcggggt aatgactcca acttactgat agtgttttat gttcagataa tgcccgatga
     5641 ccttgtcatg cagctccacc gattttgaga acgacagtga cttccgtccc agccttgcca
     5701 gatgttgtct cagattcagg ttatgccgct caattcgctg agtgtaacgc ttgctgatta
     5761 cgtgcagctt tcccttcagg cgggattcat acagcggcca gccatccgtc atccatacca
     5821 cgacctcaaa ggccgacagc aggcttagaa gacgctccag tgtggcca
//
LOCUS       DAETPZ010000166         5860 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.168, whole genome shotgun sequence.
ACCESSION   DAETPZ010000166 DAETPZ010000000
VERSION     DAETPZ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.168"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            83..442
                     /locus_tag="K0W59_003960"
     CDS             83..442
                     /locus_tag="K0W59_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HBI3024760.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(562..741)
                     /locus_tag="K0W59_003961"
     CDS             complement(562..741)
                     /locus_tag="K0W59_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HBI3024761.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            815..2176
                     /gene="pabB"
                     /locus_tag="K0W59_003962"
     CDS             815..2176
                     /gene="pabB"
                     /locus_tag="K0W59_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HBI3024762.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2180..2758
                     /locus_tag="K0W59_003963"
     CDS             2180..2758
                     /locus_tag="K0W59_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBI3024763.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHTGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            2942..4306
                     /gene="sdaA"
                     /locus_tag="K0W59_003964"
     CDS             2942..4306
                     /gene="sdaA"
                     /locus_tag="K0W59_003964"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBI3024764.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4437..>5860
                     /locus_tag="K0W59_003965"
     CDS             4437..>5860
                     /locus_tag="K0W59_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBI3024765.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPEATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEG"
BASE COUNT         1424 a         1464 c         1473 g         1499 t
ORIGIN      
        1 cattatctga acataaaaca ctatcagtaa gttggagtca ttaccaactc gtgccatatc
       61 gttataatcc ctttgatatt agatgcaaat taaggtcata tatagcctta tcgataacat
      121 ggttaatttt aaggataaga atatgcctgc tgtaatagat aaagccctgg atttcattgg
      181 tgccatggat gtatcagcgc caacaccaag ttcgatgaat gaaagcacgg cgaagggaat
      241 ctttaaatat ttaaaagaac tgggagtacc cgcgagtgcc gctgatatta cggcgcgagc
      301 tgaccaggaa ggctggaacc ctgggttcac ggaaaaaatg gttggatggg caaaaaaaat
      361 ggagacaggt gaacgctctg tgattaaaaa tcctgaatac ttttcaacat atatgcagga
      421 agaactaaaa gcactggtct gagttaaatt tatatcagca taaatgggtc aggacgcttt
      481 taatcacata taaaaagcgt cctttttctc acaatcaaat gatcatcggc ttctataacg
      541 aaatcgatac ggattagtgt gttactcgtc ttcatcctca aaacgcgcca cgattagctc
      601 gccggagtgg ttggcacgca gctcttctgc caccagcgcg attgcctggc cgctactcat
      661 cccttgtgcc atcagttcct ggatccgctc gacagctttt tgctgctgtt catgggtgag
      721 tgaaggtaaa cctgcaaaca ttgttaactc ctgctaaatt gttggcgcta attatttcat
      781 gctacccggc acatagccag tagagtcagg actgatgaag acgttatctc ccgctgtgat
      841 tactttaccc tggcgtcagg acgccgctga attttatttc tcccgcttaa gccacctgcc
      901 gtgggcgatg cttttacact ccggctatgc cgatcatccg tatagccgct ttgatattgt
      961 ggtcgccgat ccgatttgca ctttaaccac tttaggtaaa gaaaccgttg ttagtgaaag
     1021 cgaaaaacgc acaacgacca ctgatgaccc gctacaggtg ctccagcagg tgctggatcg
     1081 cgcagacatt cgcccaacgc ataacgaaga tttgccattt cagggcggcg cactggggtt
     1141 gtttggctac gatctgggcc gccgttttga gtcactgcca gaaattgcgg aacaagatat
     1201 cgttctgccg gatatggcag tgggtatcta cgattgggcg ctcattgtcg accaccagcg
     1261 tcatacagtt tctttgctga gtcataatga tgtcaatgcc cgtcgggcct ggctggaaag
     1321 ccagcaattc tcgccgcagg aagatttcac gctcacttcc gactggcaat ccaatatgac
     1381 ccacgagcag tacggcgaaa aatttcgcca ggtacaggaa tatctgcaca gcggtgattg
     1441 ctatcaggtg aatctcgccc agcgttttca tgcgacctat tctggcgatg aatggcaggc
     1501 attccttcag cttaatcagg ccaaccgcgc gccatttagc gcttttttac gtcttgaaca
     1561 gggtgcaatt ttaagccttt cgccagagcg gtttattctt tgtgataata gtgaaatcca
     1621 gacccgcccg attaaaggca cgctaccacg cctgcccgat cctcaggaag atagcaaaca
     1681 agcagaaaaa ctggcgaact cagcgaaaga tcgtgccgaa aatctgatga ttgtcgattt
     1741 aatgcgtaat gatatcggtc gtgttgccgt agccggttcg gtaaaagtac cagagctctt
     1801 cgtggtggaa cccttccctg ccgtgcatca tctggtcagc actataacgg cgcgactacc
     1861 agaacagtta cacgccagcg atctgctgcg cgcagctttt cctggtggct caataaccgg
     1921 ggctccgaaa gtacgggcta tggaaattat cgacgaactg gaaccgcagc gacgtaatgc
     1981 ctggtgcggc agcattggct atttgagctt ttgcggcaac atggatacca gcattactat
     2041 ccgcacgctg actgccatta acggacaaat atactgctct gcgggcggtg gaattgtcgc
     2101 cgatagccag gaagaagcgg aatatcagga aacttttgat aaagttaata agatattacg
     2161 ccaactggag aagtaaaacg tggaataccg tagcctgacg cttgatgatt ttttatctcg
     2221 ctttcaactt ttgcgcccgc aaattaaccg ggaaacccta aatcatcgtc aggctgccgt
     2281 gttaatcccc atcgtccgtc gaccacaacc ggggttgttg ctgactcagc gttcaattca
     2341 tctgcgtaaa cacactggac aagtggcatt ccctggaggt gcagtcgatg acacggacgc
     2401 atcagttatc gccgccgcgc tgcgcgaagc tgaagaagag gtcgctatac cgccttccgc
     2461 cgttgaagtt atcggcgtgc tgccgcccgt cgatagcgta actggctatc aggtaacccc
     2521 agtggtcggc attatcccgc ccgatctgcc gtatcgcgcc agtgaagatg aagtctcggc
     2581 ggtgtttgaa atgccgctcg cccaggcatt acatctgggt cgttatcacc ctttagatat
     2641 ctaccgccgt ggcgattcac atcgggtatg gctgtcctgg tacgaacagt attttgtatg
     2701 gggaatgacc gcaggcataa ttcgtgagct ggcgctgcaa attggtgtga acccctgact
     2761 atacttatct ttacatctac aaaacactac ttgagacaat catcgcaata ttagttaaat
     2821 cgcggttttt gattagttta attcatgtga atagttaagc cagtcgccgc gttccctctt
     2881 acactatgcg ctgttattag ttcgttactg gaagtccagt caccttgtca ggagtattat
     2941 cgtgattagt ctattcgaca tgtttaaggt ggggattggt ccctcatctt cccataccgt
     3001 agggcctatg aaggcgggta aacagttcgt cgatgatctg gtcgaaaaag gcttactgga
     3061 tagcgttact cgcgttgccg tggacgttta tggttcactg tcgctgacgg gtaaaggcca
     3121 ccacaccgat atcgccatta ttatgggtct tgcaggtaac gaacctgcca ccgtggatat
     3181 cgacagtatt cccggtttta ttcgcgacgt aaaagagcgc gaacgtctgc tgctggcaca
     3241 gggacggcat gaagtggatt tcccgcgcga caacgggatg cgttttcata acggcaacct
     3301 gccgctgcat gaaaacggta tgcaaatcca cgcctataac ggcgatgaag tcgtctacag
     3361 caaaacttat tattccatcg gcggcggttt tatcgtcgat gaagaacact ttggtcagga
     3421 tgctgccaac gaagtgagcg tgccgtatcc gttcaaatct gccaccgaac tgctcgcgta
     3481 ctgtaatgaa accggctatt cgctgtctgg tctcgctatg cagaacgaac tggcgctgca
     3541 cagcaagaaa gagatcgacg agtatttcgc gcatgtctgg caaaccatgc aggcatgtat
     3601 cgatcgcggg atgaacaccg aaggtgtact gccaggcccg ctgcgcgtgc cacgtcgtgc
     3661 gtctgccctg cgccggatgt tagtttccag cgataaactg tctaacgatc cgatgaatgt
     3721 cattgactgg gtaaacatgt ttgcgctggc agttaacgaa gaaaacgccg ccggtggtcg
     3781 tgtggtaact gcgccaacca acggtgcctg cggtatcgtt ccggcagtgc tggcttacta
     3841 tgaccacttt attgaatcgg tcagcccaga catctatacc cgttacttta tggcagcggg
     3901 cgcgattggt gcattgtata aaatgaacgc ctctatttcc ggtgcggaag ttggttgcca
     3961 gggcgaagtg ggtgttgcct gttcaatggc tgctgcgggt cttgcagaac tgctgggcgg
     4021 tagcccggaa caggtttgcg tggcggcgga aattggcatg gaacacaacc ttggtttaac
     4081 ctgcgacccg gttgcagggc aggttcaggt gccgtgcatt gagcgtaatg ccattgcctc
     4141 tgtgaaggcg attaacgccg cacggatggc tctgcgccgc accagtgcac cgcgcgtctc
     4201 gctggataag gtcatcgaaa cgatgtacga aaccggtaag gacatgaacg ccaaataccg
     4261 cgaaacctca cgcggtggtc tggcaatcaa agtccagtgt gactaatact tcttactcgc
     4321 ccatctgcaa cggatgggcg aatttatacc cgctttctcg tctgctgtaa tattccccac
     4381 tacacttcca ctgttgcgtc aggcgtttgt cgccatacgc ttacagggtg gcccgcatgc
     4441 aaaaagcaca acggatcatt aaaacctatc gccgtaatcg aatgattgtt tgtacgattt
     4501 gcgcactcgt tacgctcgct tcgaccctga gcgtgcgatt tatttcacag cgtaacttaa
     4561 atcaacaacg ggtagtacaa ttcgccaatc acgctgtaga ggaattagat aaagtactgc
     4621 ttcctctaca ggcaggtagc gaagtcttgc ttccgctgat tggtctgccc tgctctgtcg
     4681 cccatttgcc attacgtaaa caggcggcaa aactccaaac tgtgcgatcc attggcctgg
     4741 tgcaagacgg cacactttat tgctccagca tttttggtta tcgcaatgtg cccgtcgtgg
     4801 acattctggc tgaacttcct gcaccgcaac cacttttacg cctgacgatc gaccgtgccc
     4861 tgattaaagg cagtccggtt ttgattcaat ggacgcctgc agcaggcagt agcaatgctg
     4921 gggtcataga gatgattaac atcgacttac tgacggcaat gctccttgag ccacaactgc
     4981 cgcaaatcag tagcgccagc ctgacggtgg acaaacggca tttgctctat ggtaatgggc
     5041 tggtagattc ccttccgcaa cctgaagaca atgaaaacta tcaggtttct tcgcaaagct
     5101 ttccttttac cattaacgta aatggtccgg aggctacggc gctggcatgg cactatcttc
     5161 caacacaatt accgctggcg gtgctgctaa gtttactggt gggctatatc gcctggctgg
     5221 cgaccgctta ccggatgagc ttttcccgcg aaattaatct gggcctggcg caacatgagt
     5281 tcgaattgtt ctgtcagcct ttgcttaatg cgcgcagcca gcaatgtatt ggtgtagaga
     5341 ttttgctgcg ctggaataat ccgcgtcagg gctggatttc accggatgtg tttattccta
     5401 tcgcggaaga acatcattta attgtgccac tgacccgcta tgtgatggca gaaaccattc
     5461 gtcagcgcca tgttttcccg atgagtagtc agtttcatgt tggcattaac gtcgcaccca
     5521 gccattttcg ccgtggtgtg ctgataaaag atctcaatca gtactggttt agcgctcacc
     5581 cgattcagca actgatcctc gaaatcaccg aacgcgatgc cttactggat gttgattatc
     5641 ggattgcccg cgagctgcat cgtaaaaacg tcaaactggc gattgatgac ttcggcaccg
     5701 gcaacagctc gttttcctgg cttgaaacat tacgtcctga cgtgctgaaa attgataagt
     5761 catttaccgc agctataggt tctgacgcgg ttaactcgac ggtgaccgat atcatcatcg
     5821 cgctggggca aagactgaat attgaactgg tggcggaggg
//
LOCUS       DAETPZ010000167         5738 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.169, whole genome shotgun sequence.
ACCESSION   DAETPZ010000167 DAETPZ010000000
VERSION     DAETPZ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.169"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            58..1518
                     /locus_tag="K0W59_003966"
     CDS             58..1518
                     /locus_tag="K0W59_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBI3024766.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVAEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1554..1757)
                     /gene="ydfZ"
                     /locus_tag="K0W59_003967"
     CDS             complement(1554..1757)
                     /gene="ydfZ"
                     /locus_tag="K0W59_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HBI3024767.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1935..2646)
                     /gene="ydfH"
                     /locus_tag="K0W59_003968"
                     /pseudo
     CDS             complement(1935..2646)
                     /gene="ydfH"
                     /locus_tag="K0W59_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
     gene            complement(2735..3481)
                     /gene="ydfG"
                     /locus_tag="K0W59_003969"
     CDS             complement(2735..3481)
                     /gene="ydfG"
                     /locus_tag="K0W59_003969"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HBI3024768.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3618..5663
                     /gene="dcp"
                     /locus_tag="K0W59_003970"
     CDS             3618..5663
                     /gene="dcp"
                     /locus_tag="K0W59_003970"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HBI3024769.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
BASE COUNT         1436 a         1363 c         1482 g         1457 t
ORIGIN      
        1 atttttattc atataacaaa acatatagct tgccatattt aaatttaagg aattgttatg
       61 ggaaataatt tgttatcagc aaaagcgaca ctccctgttt atgatcgtaa taacctggcc
      121 ccaagaattg ttcatttagg ctttggtgca tttcaccgtg cgcatcaggg cgtgtatgcc
      181 gatattcttg ctacagaaca tttcagtgac tggggatatt atgaggtcaa cttaatcggc
      241 ggcgaacagc aaattgccga tttacaacag caagataatc tttataccgt tgcggaaatg
      301 tcggccgatg cgtggacggc tcgcgtcgtt ggcgtcgtta aaaaagcctt gcacgtacag
      361 atggatggct tagaaaccgt gttggctgcg atgtgtgaac cgcaaatcgc gattgtctct
      421 ctgacaatca ccgaaaaagg gtatttccac tctccggcga ccggacagtt aatgctcgat
      481 cacccgatgg tcgctgccga cgtgcaaaat ccccaccagc cgaaaacagc aacaggggtg
      541 attgttgagg cgctggctcg ccgtaaagcg gcaggacttc ccgcatttac cgtcatgtca
      601 tgtgacaaca tgccagaaaa cggtcatgtt atgcgtgacg ttgtcacttc ctacgcgcaa
      661 gccgttgatg taaaactggc acaatggatc gaagataacg tgactttccc atcaacaatg
      721 gtggaccgta ttgtgcccgc agtggcagag gatacgctgg cgaaaatcga acaacttacc
      781 ggtgtgcgcg atcctgctgg cgttgcctgt gaacctttcc gccagtgggt tatcgaagat
      841 aactttgtag ccggacgtcc ggaatgggaa aaagcgggag ccgaactggt tagtgatgtg
      901 ctgccttatg aagagatgaa gttgcgcatg ctcaacggca gtcattcatt cctggcatat
      961 ctgggttatc ttgctggata tcagcacatt aatgactgta tggaagatga acattatcgt
     1021 catgcggcgt atggcatgat gttgcaggaa caagcgccga cgctgaaagt gcagggcgtt
     1081 gatttgcaag attacgctaa ccgattaatt gcacgctata gcaacccggc gttacgtcat
     1141 cgaacctggc agattgcgat ggatggcagc cagaaattgc cacagcggat gttggattct
     1201 gttcgctggc atctggcgca tgacagcaag ttcgatctgc tggcgctggg cgtcgcgggt
     1261 tggatgcgtt atgtcggtgg tgttgatgaa cagggaaatc cgatagaaat cagtgaccca
     1321 ctgttacctg ttattcagaa ggctgtacaa agtagtgccg aagggaaagc gcgcgtccag
     1381 tcattgctgg cgattaaggc aatctttggt gatgatttgc cagacaatag cttgtttact
     1441 gcaaaagtga cggaagcgta cttgtcttta ttagcgcatg gcgcgaaagc gaccgtggcg
     1501 aaatattccg tgaagtaaaa aaagcaatgc cgccatcggc ggcattcaca cacttagttc
     1561 atgccgagac gaatcaggtc cagaggtgcc agtttctctt cacaaccttc aacaactacg
     1621 gtttttccgc ggcggatttg ctcagcggtc agaccttcag tatcaatcga caggatcttg
     1681 ccagtgtgac cggtgccgct aaccataaca cggctgccag tggtgattgc gttacggtta
     1741 cgatcgtagg tcgtcatagg tgttttctcc tttctgattt acagtcgaag cccacgtaac
     1801 gggcgcaata taaatactcc tgtcgagtga aatatttttt gtttttgatc aagctcacat
     1861 cttttttgat gcatgcatga gccgtaaggc ggggggaaaa gcctctcatc ccggatggga
     1921 tgagagtaaa tgaattactc ttcgctaaac cagttgctgt tttcctggcg gatctggcgc
     1981 acggattcgc tgatttcctg caaatgttgc gtcattgcac gttctaccgc atcgccatca
     2041 cgtttttgca gggcagagaa aatatcaaga tgctggcgta acagcatttc tggtggagaa
     2101 acatggtcga aactcatata gcgcacgcga tcaacggtcg ctttcaggtt ctcaatggta
     2161 tcccacgcca gttgacagtc ggcaatctgc gtcaggagtt gatggaagtt gtcatcaagt
     2221 tcaaaaaaat catccagttg cttgcgctca atggcaatgc gttgctggtg aagattttgt
     2281 tccagttggt agcactggct ttcggtaatc atgctcgcac tggctttcgg taatcatgct
     2341 cgccgcccga cgcgccaccg cgcactcaat ggcctgacgg ataaaactgc cgttgcgcac
     2401 ctgggccatg gaaattttgt tgacgtagct gccacgctgc ggacgaattt gaatcaggcc
     2461 gttttccgcc agtttaataa aggcttcacg aaccggctgg cgtgacacat tgaaacgaac
     2521 agaaacttct ttttccgaca acggtgtgcc tggtgcaatc aggcaatgga caatgtcgcg
     2581 acgaagaata cgataaatct gctgattgac aggctgtgtg ggattaagtt gcgtttcgac
     2641 ggtcattcgt tcttgcttgt gagtgagtta actgcatgag tctaccactt ttttgtgaca
     2701 agcaataacc cggcagttac gccgggtata aaaattactg acggtggaca ttcagtccgg
     2761 catagctttg ggtaaccggc atcatttcca gggtattgat attgacgtga gcaggcagcg
     2821 ttgacaccca ccagacggct tcgctgacat cttctggcgt caatgcaacg gtattttgat
     2881 aggttttttc cgctttaccg tcatcgcctt taaagcggac attggaaaac tcggtgccac
     2941 ccaccagacc cggttcgatg tcggtgacgc gcaccgccgt accatgcaga tccgtacgca
     3001 gattcaggct aaactgacga acaaacgctt tcgtcgcacc gtaaacgtta ccaccggcat
     3061 acggccagct gcctgccgtt gagccaatgt taataatatg accatgatta cgttcaacca
     3121 tacccggtaa gacggcgcgc gtcatatata ctaggccttt gttgttggta tcaatcatcg
     3181 tttcccagtc ttcaacgctg gctttatgcg caggctccat gcccaacgcc aggccggcat
     3241 tatttaccag gatatcaata ttgctccact cggcagggag cgatgccagc atctcttcaa
     3301 tagcggcgcg gttgcgaacg tccagttggg cgatatacag attatctccc agttcgtctt
     3361 ttaactcctg caaccgctcc tggcgacgcc cagtggcgat aactttatgc ccttgttgaa
     3421 taaaacgacg agtaatgcat tcaccaaaac ctgccgttgc tccagttact aaaacgatca
     3481 tctcactgtt cctcaacgct tttgtgtgtc aataacatag cacgtgatgc ttaagagcgg
     3541 gtaatactgg tttgcaagat tgcactccgt tatcagtaag cctacgctat ggaacacctc
     3601 gcgttcagga gaagaaaatg acaacaatga atcctttcct tgtgcaaagc acactgccgt
     3661 atctggctcc tcattttgat caaattgcca atcatcacta tcgcccggca ttcgatgagg
     3721 gaatgcaaca aaagcgggca gaaattgctg ccatcgcgct taacccgcaa acgcctgatt
     3781 tcaacaatac tattctggca ctggaacaaa gcggagaatt aattacccgc gttaccagcg
     3841 tcttttttgc gatgactgcg gcgcatacca atgatgaatt acagcgtctt gatgaacagt
     3901 tttccgctga actggcggaa ctggctaatg atatctatct gaacggtgaa ttattcgcgc
     3961 gggtagatgc tgtctggcag cgccgtgaat ccctggggct tgatagtgaa tccatccgcc
     4021 tggtggaggt tattcatcaa cgttttgtcc ttgccggagc caaacttgca caagctgata
     4081 aagcaaaatt aaaagtactg aatacagaag ctgcgaccct gaccagccag tttaaccagc
     4141 ggttacttgc agcaaataaa tccggcggtc tggttgtgaa cgatatcgcg cagctggcag
     4201 gaatgagtga gcaagagatt gcgctggcgg cagaggcggc tcgcgagaaa ggtctggata
     4261 acaaatggct gattccgctg ctgaatacca cccaacaacc ggcgcttgcc gaaatgcgcg
     4321 atcgtgcgac gcgtgaaaaa ctgtttattg cgggctggac gcgagcggaa aaaaatgatg
     4381 ccaatgatac ccgcgctatc attcaacgtc tggtagagat tcgcgcacag caggcgaaac
     4441 tgcttggttt tcctcattat gccgcatgga aaatcgccga tcagatggca aaaacgccag
     4501 aagcagcact caactttatg cgggaaattg ttccagcggc gcgtcaacgt gcgagcgatg
     4561 aattagcctc catacaggcg gttatcgata agcagcaggg cgggtttagc gcgcagccgt
     4621 gggactgggc attttatgcg gaacaggtac ggcgggagaa atttgatctt gatgaggcgc
     4681 agctcaagcc atattttgaa ttaaacacgg tgttaaatga aggtgtattc tggaccgcga
     4741 atcagctctt cggtattaag tttgtcgaac gttttgatat tccggtctac catcctgacg
     4801 ttcgtgtgtg ggaaattttt gatcataatg gcgtgggact ggcgttattt tacggtgatt
     4861 tcttcgcccg tgattcaaaa agcggcggtg catggatggg caattttgtt gagcaatcaa
     4921 cgcttaatga aacgcatccg gtaatttata acgtctgcaa ttatcagaaa cccgctgccg
     4981 gtgagcctgc gttgttactc tgggatgatg taataacctt attccatgaa tttggtcata
     5041 cgctgcacgg cctttttgcc cgccagcgtt atgccacgct ttccggcacc aacacgccgc
     5101 gtgattttgt cgaatttccg tcgcaaatca acgaacactg ggcaacgcat ccgcaggtat
     5161 tcgctcgcta cgcccggcat tatcagagcg gggcagcaat gcctgacgaa ctgcaacaga
     5221 aaatgcgtaa tgccagcctg ttcaacaaag ggtatgagat gagcgaactg cttagcgccg
     5281 cacttctcga tatgcgctgg cattgcctgg aagaaaacga agcaatgcag gatgtcgatg
     5341 attttgaatt gcgggcgctg gtggcggaaa atatggatct tcctgctata ccgccacgct
     5401 atcgcagcag ttatttcgcc catatttttg gtggcggata tgctgcaggt tattacgctt
     5461 atctgtggac gcaaatgttg gccgatgatg gttaccagtg gtttgttgag cagggcggat
     5521 taacgcgtga aaatgggcag cgttttcgcg aggcgatcct ttccagaggt aacagcgaag
     5581 atctggaacg cctgcatcga caatggcgcg gtaaggctcc tcagattatg ccgatgctgc
     5641 aacatcgtgg cttgaacata taaacaccac caatggctgg cctggagata tcgccaggcc
     5701 agcctggtta tagcagtaat tgcagcatgt tacgacaa
//
LOCUS       DAETPZ010000168         5716 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.170, whole genome shotgun sequence.
ACCESSION   DAETPZ010000168 DAETPZ010000000
VERSION     DAETPZ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.170"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..297
                     /locus_tag="K0W59_003971"
     CDS             <1..297
                     /locus_tag="K0W59_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HBI3024770.1"
                     /translation="KKNKQRKEQKPFLIPLLNPKAYLFFAALIPTFIDNNTNITLNFF
                     ILGVLFIFISFLTDLIYIAISLTIRDKLTPSFSRYISICSSIFILGTGIYFIFT"
     gene            complement(355..1071)
                     /locus_tag="K0W59_003972"
     CDS             complement(355..1071)
                     /locus_tag="K0W59_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024771.1"
                     /translation="MQLDEHSRKIAALVAYNDKQIKEMNRLITLLNERVNDLNTFTST
                     LEREGKVVSGISYSFRDSVSNVKFYINKGMDEASCKLQKSVSDTLTSLLREKLSFTLN
                     DVKKEWVDFEEQTSKEIKESLKQTTREAEVATGIIRKTMEKYTVTRIVEKVIVLIVLF
                     LLLMIAPVFYAEYLKMNISSLMEEQKKHESMLKEWKEKAPLAEFSKCGDYRKACVLVD
                     DESGYYFNEQSPNKKYAILK"
     gene            complement(1092..2177)
                     /locus_tag="K0W59_003973"
     CDS             complement(1092..2177)
                     /locus_tag="K0W59_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBI3024772.1"
                     /translation="MASYHCTVKTGAKGVALKHADYISRSGEYKNYKSREDLEFSSSG
                     NMPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKK
                     ARFGETPQERKSALVALRSHWADVQNEHLARHGHQSRVDHRSLKEQGIKRTPEVHLGP
                     VRVAVMNADQVCAILARRNAEREKLEAEQERDSIIDLKTTLKEALAERDNIPPSLDEL
                     GAELKAFVESSFDELNEVMQLNSLINGALDSMMKQFQVEEERKLQQERLIEAEKQRDL
                     ERERERVRQRAYSSPAPSRSRDYGMSR"
     gene            2349..2795
                     /locus_tag="K0W59_003974"
     CDS             2349..2795
                     /locus_tag="K0W59_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HBI3024773.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKT
                     LSALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVD
                     SKTGKPKFSAAELVGALAGIAELPRNHPKWQEWEKRGKELLTKDSA"
     gene            complement(3002..3931)
                     /locus_tag="K0W59_003975"
     CDS             complement(3002..3931)
                     /locus_tag="K0W59_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBI3024774.1"
                     /translation="MSAVLQLFREKLPHKPYCTNDFTYGVRILPKDIAILARFIQQNQ
                     PHAQHWLAFDVDRTGAAIDWSDRNCPAPNITVKNPRNGHAHLLYALTLPVRTAPDASA
                     SALKYAAVIERALCEKLGADVNYSGLICKNPFHQEWQVVEWRWELYTLDELADYLDLS
                     ASARRRVDKDYGMGRNWYLFEKGRKWSYRAIRQGWPAFSPWLDAVIQRVEMYNASLPV
                     PLSPAECRAIGKSIAKYTYRKFSPEGFSAVQAARGRKGGNANSSENQAIKGQKSKRVA
                     VATSARTLKPWVTLGISRATYYRKLKCDPDLAK"
     gene            4484..5434
                     /locus_tag="K0W59_003976"
     CDS             4484..5434
                     /locus_tag="K0W59_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024775.1"
                     /translation="MNNFINECISAPRINGMAEFFSSTASGEKHKPFRLVEIDDRLFH
                     SYDYIKFAKHLTKNSGPFVNHYFCSIPYSKEEECRLGTTVIKYAITLNKKLNTWCLGM
                     AEGAMARTISELSKGNIQTLTTSPTKENEHAFFKYGSSENAYFICTPFFLLEERLKNS
                     NLKQFNQGFDIIIEDTTFQMYSPNRSGQINHVKRLLNNDGIFIFTEKHSSEHDEYQKR
                     EIQKDYSFKQRYFSKDEINTKKEIVLNTMNLNEVSVDDMLSAIKQHFKYASIYWNSGN
                     FYSIAASNSLKHISTFINLLPKAAIPMEYVYEKLPRNISW"
     gene            5441..>5716
                     /locus_tag="K0W59_003977"
     CDS             5441..>5716
                     /locus_tag="K0W59_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HBI3024776.1"
                     /translation="MEYSTLLSFAIVTLSQTISIGPGVALVINNAFSHGLKSSIKTSI
                     YIRIGETIVMAISLFALSSTSSTEQHFHIIKIFGGGYLIYIGLMGLIN"
BASE COUNT         1713 a         1312 c         1108 g         1583 t
ORIGIN      
        1 aaaaaaaaca aacaaagaaa agagcaaaaa ccttttttga taccattatt aaaccccaag
       61 gcgtatcttt tctttgctgc gttaatacca acctttatag ataataatac aaatatcaca
      121 ttaaactttt ttatattggg tgttttattt atatttatat cattcctaac agatcttatt
      181 tacatagcta ttagcttaac gataagggat aaattgacgc catcattctc aagatacata
      241 agcatatgta gttccatttt tatattaggt acaggtattt attttatttt tacgtgaaac
      301 gccgttttta tactcatgca gccttagtca taagacggca tgagttactt aacactattt
      361 cagtatggca tattttttat ttggtgattg ttcattaaaa taatatccgc tttcgtcatc
      421 aactaacacg catgctttac gataatctcc acacttcgag aactcggcca atggagcttt
      481 ttctttccat tcttttaaca tggattcatg ttttttctgt tcttccatta gcgaactaat
      541 attcattttg agatattcag catagaaaac aggagcgatc attagcaata aaaataatac
      601 aatcaacaca attacctttt cgacaatcct tgttactgta tacttctcca ttgtttttct
      661 aattatacct gtcgcaactt cggcctctct ggtcgtctgc tttaatgatt cctttatctc
      721 tttacttgtt tgctcctcga aatcaaccca ctctttcttt acatcattga gagtgaaaga
      781 taatttttcg cggagtaatg acgttagtgt atctgagact gatttctgta atttacaaga
      841 tgcctcatcc attcctttat taatataaaa tttaacatta gacacactat ccctaaagga
      901 ataagaaatt ccagaaacaa ctttaccttc acgttcaaga gtacttgtaa aggtatttaa
      961 gtcattcact ctttcattaa gcagagtaat tagcctgttc atttctttta tttgtttatc
     1021 attatacgca acaagagcag caatttttct tgaatgttca tccaattgca taaaataaaa
     1081 acctccagtg cttaacggct cataccataa tcacgtgaac gtgatggtgc tggtgaggaa
     1141 tacgcacgct ggcgcactct ttctctttcc ctctccaggt ctctttgctt ttcggcttca
     1201 attagtcgtt cctgttgcaa cttccgctcc tcttccacct ggaattgctt catcattgag
     1261 tccagcgcac cgttaatcaa tgaattaagc tgcattacct cattgagttc atcaaacgag
     1321 ctttcaacaa acgccttcaa ttctgcccca agctcatcca ggcttggtgg tatgttatcc
     1381 cgttcagcta acgcttcctt caaggtcgtt ttaaggtcaa tgatgctgtc ccgttcctgc
     1441 tctgcttcta gcttctcccg ttccgcgttt ctccgtgcca gaattgcaca aacctgatca
     1501 gcattcataa cagcaacacg aacgggacca agatgaactt ccggtgtgcg cttgataccc
     1561 tgttctttca gtgaacgatg atcaacgcgc gactgatggc cgtgccgcgc aaggtgttca
     1621 ttctgcacat cagcccagtg agaacgtagc gcaaccaagg ctgattttcg ctcttgtggt
     1681 gtctcaccaa agcgtgcctt ttttgcacct ccccgctccg gtgatttgct gttggcacgt
     1741 ttaaaaaact gctgcggatc gcgttcaata ccatcattaa tccgctcgca aaacatgata
     1801 tgtgcgtgtg gttgttcctt tccgtcgatt gcacctggcg gattgtggat cgcgtactga
     1861 taggcatggc gatcaccaag ctccttttgc acgaaatctt caacaagttc tatccgttgt
     1921 tcgcgtgtca gttctcgcgg taatgcgatc tcaatttcac ggtatgccgt cccgtttttt
     1981 cgttcaaatt catctgctgc tttccatagc tctgcgggat ttttcttcgc ccatgatggc
     2041 atgttaccgg atgacgaaaa ttccagatcc tcccgcgatt tgtagttctt atattcgccg
     2101 gatctagaaa tataatcagc atgttttaag gctacgcctt ttgctccagt tttaactgta
     2161 caatggtagc ttgccatctg ccgttaagcc ctccgcagga gcgcactgtt tctcgacgaa
     2221 ggagagaaac gtctaagtgc gcatccctga attaatcagg gatgatttta aggtctcaca
     2281 cgcaggcaga caaggtgata ttatgcacaa taacaactca cattaacggc aaaagtgcag
     2341 aggtaagaat gacaaatcgt gaagagtggt taagtgcaaa aattgcctat atcaacggac
     2401 tgaaatcacc gtcagagcag caaagattac ttgtgttact ggcagagaaa aaaaaccgaa
     2461 caacgacgga tgaaaaaacg ttgtccgcac tgatacgggc agaaaaaacc gcagagaaag
     2521 cagccgcagc aaaagccagg gtaacggcaa taatagcggc agaacggaag gcagccgcaa
     2581 gggcggaacg aaaggcgcgg gatcatgaac tctacaaggc cgcaggcctc atgattgtgg
     2641 caggactggt ggacagcaaa accggaaaac caaagtttag tgctgccgaa ctggtaggcg
     2701 cactcgcagg aatcgcagag ctaccgcgca accatccaaa atggcaggaa tgggaaaaac
     2761 gcgggaaaga gctactcacc aaagacagtg cgtaagagcc tccgttcacg ttccggttcc
     2821 ctggtgcttc ctcgctcatt cgctcgctac gctcggtcat tgagactgcg gcgagcacaa
     2881 ccaccactca cacacagacg ctaaaaccat tgccaaacga aacacgggaa atcaccccaa
     2941 acctacccgc tcaaaaaaac gcctacaggc gctgttatct gataaggctt gttatctggt
     3001 ctcattttgc gagatctgga tcacatttta gttttcgata gtatgtggca cgactgatcc
     3061 ctaacgtcac ccacggcttc agagttcgtg ccgatgtggc aacggctaca cgctttgatt
     3121 tctgaccttt aatagcctga ttttctgatg aatttgcatt accgccctta cgacctcgag
     3181 cagcctgtac cgcggaaaag ccttcaggag agaatttgcg gtaggtgtat ttcgcaatac
     3241 tcttgccaat agcccgacat tcagccggag aaagcggaac gggaagcgat gcgttgtaca
     3301 tttcgacacg ctgaatcact gcatcaagcc acggtgagaa tgctggccag ccctgacgaa
     3361 tcgccctgta agaccattta cggccctttt cgaacagata ccagtttcgc cccatcccgt
     3421 aatctttatc gacgcgacgg cgcgctgagg cgctcaaatc aagataatcc gccagttcgt
     3481 cgagggtata gagttcccaa cgccattcca ccacctgcca ttcctggtgg aacggatttt
     3541 tgcagatcag gccgctgtag ttcacatccg cgcccagttt ttcacacagc gcacgctcaa
     3601 tcacggcagc gtatttgagc gccgaagccg atgcatccgg cgcggttctc accggaagag
     3661 tgagcgcgta gagcagatgc gcgtgcccgt tacgtggatt ttttacggtg atgttcgggg
     3721 ccggacagtt ccggtcgctc cagtcgatcg ctgcccccgt ccggtcaacg tcaaaggcca
     3781 gccagtgctg cgcatgtggc tggttctgct ggatgaaacg ggcaaggatg gcgatatctt
     3841 tcggtagaat gcgaacgccg tacgtgaaat cgttcgtaca gtacggtttg tgcggtaatt
     3901 tttccctgaa gagttgaagt acggcgctca tgtgttagga tttcctccgt gtgttacgac
     3961 ccacacgccc cttgaatttt cgtgacagtc ttggcggatc ggcacgaatt tcaggggcgt
     4021 ttgtttttca gttggttaca ctacacccaa cggcaaatcc tgtcgatcct tcccgaaaac
     4081 cagacccatt taccataact cctcttgtac gtaccctcta aaacgctctc aatcgctttc
     4141 tgacgcaaaa aactcaaaaa gacaagataa acctagggaa aacacaaaaa acgcgtcaga
     4201 atcgttttta ggggcctgca taactatgca caaaagcgat taatgatcgg tttcacccct
     4261 tcagaagcgc gaactggtca aaacagcaaa ctttctcatc cagaaagcca gctgaactaa
     4321 cccgcatgaa aacaaatttc caaacatatc cagtctggac aacgaaatat cgacaatata
     4381 aaacatcata atcgtgttta tggcgtgtaa aaaagcttac aagagaatta gcattgaaga
     4441 gtcataacaa caaattccaa aaatcaaacc agaggatagg aaaatgaata attttataaa
     4501 tgaatgtatt tccgctccga gaataaacgg catggcagaa tttttctcat caactgcatc
     4561 aggggaaaaa cacaaacctt ttagactagt agaaatagat gatcggcttt ttcattctta
     4621 tgattacatt aagtttgcca aacacctcac aaaaaacagc ggccctttcg ttaatcatta
     4681 tttttgcagt ataccataca gcaaggaaga agagtgcaga ttaggaacaa cagtaataaa
     4741 atacgcaatc acgctaaaca aaaaattaaa cacatggtgt cttggtatgg ctgagggcgc
     4801 aatggcaaga accatttcag aattatcaaa aggaaacata caaacattaa caaccagtcc
     4861 aacaaaagaa aatgaacatg cattttttaa gtatggctca agtgaaaacg cttattttat
     4921 ttgcactcca tttttcctgt tagaggaaag actaaaaaat tcaaatctta aacaatttaa
     4981 tcaaggattc gatattataa tagaagacac cacatttcaa atgtactcac ctaacagatc
     5041 tgggcaaata aaccacgtaa aaaggctatt aaataatgat ggtattttca tatttacaga
     5101 aaaacatagt tcagaacatg atgaatatca aaaaagagaa atccaaaaag attattcatt
     5161 caaacaaagg tatttctcga aagatgagat taacaccaaa aaggaaatcg tactaaacac
     5221 aatgaatttg aatgaagtta gtgttgacga tatgctttcc gcaattaaac aacattttaa
     5281 atacgcatca atatattgga atagcggaaa tttttactca atcgccgcca gcaattcact
     5341 aaaacacata tcaacattta taaatttatt accgaaagct gcaattccta tggaatatgt
     5401 atatgaaaag ctcccacgaa atatatcatg gtaagtaaac atggaatatt caacattatt
     5461 atcatttgct atcgtaacct tatcgcaaac tatatcaata gggcctggcg ttgcattggt
     5521 tataaacaat gccttttcac atggattaaa gtcatcaata aaaacaagta tatacataag
     5581 aattggcgag actatcgtca tggcaatatc tcttttcgcg ttatcctcaa catccagcac
     5641 agaacaacat tttcacatta taaaaatatt cggtggaggc tatttaatat acataggatt
     5701 aatgggttta attaat
//
LOCUS       DAETPZ010000169         5600 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.171, whole genome shotgun sequence.
ACCESSION   DAETPZ010000169 DAETPZ010000000
VERSION     DAETPZ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.171"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            49..684
                     /locus_tag="K0W59_003978"
                     /pseudo
     CDS             49..684
                     /locus_tag="K0W59_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
     gene            complement(738..1343)
                     /gene="azoR"
                     /locus_tag="K0W59_003979"
     CDS             complement(738..1343)
                     /gene="azoR"
                     /locus_tag="K0W59_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBI3024777.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1544..5446
                     /gene="hrpA"
                     /locus_tag="K0W59_003980"
     CDS             1544..5446
                     /gene="hrpA"
                     /locus_tag="K0W59_003980"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HBI3024778.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNVRHTEILPLYARLSNSEQNR
                     VFQSHSGRCIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANDGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1386 a         1370 c         1554 g         1290 t
ORIGIN      
        1 tctatttcag gaaggagtgt ccgttaaact caggctacct cagtcgtagg cgcgtgcatt
       61 gtgttgatgg agctaatgat gatggttcag ttatggtggt cagtctggtt atgttggcca
      121 gtattatcgc tactcattgt tggccgtggg tgcggtgggc ttggcgcgat aacaacaggg
      181 aaagatagtc aggggaaact cccgcccgcc gtttactggc tgacattgcc ctggcgcatc
      241 gggatgtggc tatctatgcg ttggttttgt cgtcgcctgg agccggtgag caaaatgact
      301 gctggtgttt atttaagggc gtttccacga catattccgg cacagaatgc ggttctggac
      361 gtcacctttg aattccctcg tggacgagcg acaaaagatc gactctattt ctgtgtaccg
      421 atgttggatc tggtggttcc ggaagagggg gagctccgac aggccgtggc gatgctggaa
      481 acattacgcg aagagcaagg cagcgttctg gtccattgcg cattgggatt atcgcgcagt
      541 gcgctggtag tggcggcatg gctgttatgt tacggacact gtaaaacagt tgatgaagcg
      601 attagcttta ttcgagccag acgctcgcat attgtgctta aggaagagca caaagcgatg
      661 ttgaaattat gggaaaacag gtaagtggat tgagatgtgg actgaatatc tacagtccac
      721 atcaagaccg tgtccggtta tgcagcaaca atgctgtcga tggctgcttt tgcgtcagac
      781 tgtgctttcg ctgccatttc cggaccgtat gcgatccctt cggcgaagac aaatttcaca
      841 tcggtaatgc cgataaagcc gaggaacgtg gacagatacg gcgtcaccag gtccgttggt
      901 ccatctttgt ggatcccacc gcggctggta ataacgatgg cttttttacc cgttaccaga
      961 ccttccggac cgttctcggt atagcggaaa gtaacgcctg cgcgtgcaac caggtcaaaa
     1021 tagtttttca actgagttga gatgttgaag ttatacatcg gtgccgcaat aacgataacg
     1081 tcgtgggctt tcagctcggc aatcagctca tcggaaagcg ccagagcttc ctgctgacgc
     1141 ggagtcagcg gcgcatcgct cggacgcaga gcgccaacca gttcgccatc cagtaccgga
     1201 atcggatttg cagctaggtc gcgaacggtg atttcatcag cggagtgctt ttcgcgccat
     1261 tgttcaacaa aataatcgga caactgatta gactgagagt accctgccag gatgctggat
     1321 ttaagaacta ataccttgct catggtgttt ccttatagat gtttgaatgg acgatgcccc
     1381 gttgcttgct gacactttat tcacaatcct gccacagaga tagcgcaata aatcgaagtc
     1441 tatgttcgaa tttattgaac aacgcataga aagccgcgat gtggtactct atatctatca
     1501 tttaaaagaa aattaatcag gcagactgct gcccactaac gttatgacag aacaacaaaa
     1561 attgaccttt actgccttgc aacagcggct ggattcgctg atgctgcgcg acagactgcg
     1621 tttttctcgc cgtctgcatt gtgttaagaa ggttaaaaat cctgatgcac aacaggccat
     1681 tttccaggag atggcgaaag agattgacca ggcggcaggg aaagtcctgc tgcgtgaagc
     1741 ggcacgaccg gaaatcactt atcctgacaa tttaccggtt agtcagaaaa aacaggacat
     1801 actcgaagcg attcgtgatc accaggtggt gatcgtcgcc ggggaaaccg gctccggtaa
     1861 aacgactcag ctaccgaaaa tctgtatgga gctggggcgc ggtattaaag ggctgatcgg
     1921 tcatacccag ccgcgtcgcc tggcggcacg aacggtggcc aaccgtattg cggaagagct
     1981 gaaaacggag ccgggcggtt gcatcggtta caaagtgcgt ttcagcgatc acgtaagtga
     2041 taacacgatg gtcaagctga tgaccgacgg gatcctgctg gcggagatcc agcaagaccg
     2101 cctgctgatg cagtacgaca ccatcattat tgacgaagcg cacgaacgca gcctgaatat
     2161 cgattttctg ctcggctatt tgaaagagtt gctgccgcgg cgtcctgacc taaaaatcat
     2221 tatcacttcc gcgactatcg acccggaacg cttttcgcgc cactttaata atgcgccgat
     2281 tattgaagtc tccggtcgga cctatccggt ggaagtgcgc tatcgcccga ttgttgaaga
     2341 agccgatgac accgagcgcg atcagttgca ggcgattttt gacgccgtag acgaactaag
     2401 ccaggaaagc cccggcgaca ttctgatctt tatgagcggc gagcgggaaa ttcgcgatac
     2461 cgccgatgcg ctgaacaagc tgaacgtacg ccataccgaa atcttgccgc tttatgcgcg
     2521 gctttcgaac agcgaacaga accgcgtgtt ccagtcgcac agcggacggt gcattgtgct
     2581 ggcgaccaac gtcgcggaaa cctcgctgac cgtaccgggg attaaatacg tcatcgaccc
     2641 cggtacagcg cgtatcagcc gctacagcta tcgcaccaaa gtgcagcgtt tgccgattga
     2701 acccatttcg caggcgtctg ccaatcagcg taaaggccgc tgtggtcgtg tgtccgaagg
     2761 gatctgtatt cgtctctatt ccgaagacga tttcctctcg cgcccggagt ttaccgatcc
     2821 ggagattctg cgtaccaacc tggcctcggt tattttgcag atgaccgcgc tggggctggg
     2881 cgatatcgct gcgttcccgt ttgtcgaagc accggataaa cgcaatatcc aggacggcgt
     2941 gcgtctgctc gaagaactgg gcgcgatcac cacggatgaa caggccagcg cctataaact
     3001 gacgccgctc ggtcgccagc tctcgcagtt gcctgtcgac ccacgtctgg cgcgtatggt
     3061 gctggaagcg caaaaacatg gctgcgtgcg tgaggcgatg attatcacgt ccgcgctctc
     3121 catccaggat ccgcgcgaac gtccgatgga caaacagcag gcatcggacg aaaaacatcg
     3181 tcgcttccac gacaaagaat ccgacttcct cgcgtttgta aatctgtgga attatcttgg
     3241 cgagcagcaa aaggcgcttt cttccaacgc cttccgtcgc ctgtgtcgta ccgattacct
     3301 caactatctg cgcgtgcgcg aatggcagga tatctacacc cagttgcgcc aggtggtgaa
     3361 agaacttggc attccggtca acagcgaacc ggcggagtat cgtgaaattc acatcgcctt
     3421 actgaccggt ttgctttccc atatcggcat gaaagatgcc gataaacagg aatataccgg
     3481 tgcacgtaac gcgcgtttct ccatcttccc cggttctggt ttattcaaaa agccgccgaa
     3541 atgggtaatg gtggcggaac tggtagaaac cagccgcctg tgggggcgca ttgctgcgcg
     3601 tatcgacccg gaatgggtag aacccgttgc tcagcatttg attaaacgca cctacagcga
     3661 accgcactgg gaacgggcgc agggcgcggt gatggcaacg gaaaaagtca ctgtttatgg
     3721 tttgccgatt gttgctgcgc gcaaggtcaa ctacagccag atcgattcgg cgttatgtcg
     3781 tgaactcttt attcgccacg ctctggtgga aggtgactgg cagacgcgtc acgcattctt
     3841 ccgtgaaaac ctgaaactgc gggccgaagt ggaagagctg gaacacaaat cacgtcgccg
     3901 cgatattctg gttgatgacg aaacgctgtt tgagttctac gaccagcgca tcagccacga
     3961 tgtaatctcc gctcgtcact tcgatagctg gtggaaaaaa gtcagccgcg aaacgcctga
     4021 tttgctcaac tttgaaaaga gcatgttgat caaagaaggg gcggaaaaaa tcagcaagct
     4081 ggattacccg aacttctggc atcagggcaa tctcaagctg cgtttgagct atcagtttga
     4141 gccaggcgcg gacgcggacg gtgtgaccgt acatattccg ctgccgttac ttaaccaggt
     4201 tgaggaaagc gggtttgaat ggcagatccc cggtctgcgc cgcgaattgg tgattgctct
     4261 gattaaatcg ttgccgaaac cggtacgccg taattttgtg cctgcaccaa actacgccga
     4321 agcgttttta ggccgcgtca caccgctgga gttaccgttg ctcgacagcc ttgagcgcga
     4381 gttacggcgg atgaccggcg ttaccgttga ccgcgaagac tggcactggg atcaggtgcc
     4441 cgatcacctg aaaatcacct tccgcgtggt ggatgacaaa aacaagaagc taaaagaagg
     4501 gcgctcgcta caagatctga aagatgcgct gaaaggcaaa gtgcaggaaa cgctgtccgc
     4561 ggtggcggat gacggtatcg agcagagcgg cttacatatc tggagttttg gtcagctgcc
     4621 ggaaagttac gaacagaagc gtggcaacta taaagtgaag gcgtggccgg cgctggtgga
     4681 tgaacgcgac agcgtggcga tcaaactgtt tgataacccg ctggagcaaa aacaggcaat
     4741 gtggaatggt cttcgccgtc tactgctgct aaatatttcc tcgccgatca agtatttaca
     4801 tgagaagtta ccaaacaaag ccaagctggg gctgtacttt aacccgtatg gcaaagtgct
     4861 ggagctgatc gacgactgta tctcctgcgg tgtggatcaa ctgatcgacg ccaatgatgg
     4921 cccggtctgg acggaagaag gctttgctgc gctgcatgaa aaagtgcgcg ccgaactgaa
     4981 tgacacggtg gtggatattg cgaagcaggt cgagcaaatc ctcactgcgg tgttcaatat
     5041 caacaaacgt ctgaaagggc gggtggatat gaccatggca ctggggcttt ctgacattaa
     5101 agcgcagatg ggcgggttgg tatatcgcgg ttttgtcact ggtaacggct tcaaacggct
     5161 gggcgacacg ctgcgatatt tgcaggcgat tgaaaaacga ctggaaaaac tggcggttga
     5221 tccacatcgc gatcgtgcac agatgctgaa agtcgaaaac gtccagcagg cgtggcagca
     5281 atggatcaac aaactgccgc ctgcacgtcg tgaggatgaa gacgtgaaag agatccgttg
     5341 gatgatagaa gagctgcgcg ttagttactt cgctcaacaa cttggtacgc cttatccgat
     5401 ttcagataag cgtattttgc aggcgatgga gcagattagc ggttaaccct gctatttgcc
     5461 taataaatct aaaacccggt aagcatttag cgccgggttt tttattaatt ctaaaacggt
     5521 aaggacaaaa attcaggaat tcagaaaaat acaattctca gctgcaagat gaataatgtt
     5581 tatctacagc atttccttaa
//
LOCUS       DAETPZ010000170         5599 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.172, whole genome shotgun sequence.
ACCESSION   DAETPZ010000170 DAETPZ010000000
VERSION     DAETPZ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.172"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..410
                     /locus_tag="K0W59_003981"
     CDS             <1..410
                     /locus_tag="K0W59_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3024779.1"
                     /translation="VRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDAL
                     CYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGID
                     PEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            442..1056
                     /gene="istB"
                     /locus_tag="K0W59_003982"
                     /pseudo
     CDS             442..1056
                     /gene="istB"
                     /locus_tag="K0W59_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="K0W59_003983"
     CDS             complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="K0W59_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HBI3024780.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTSGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSIVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKTFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYTGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1379 a         1402 c         1329 g         1489 t
ORIGIN      
        1 ccgtcaggca gcagcgaagc aaagcgttac tgacgtcgct gaatgaatgg atggtggaga
       61 agaatggtac gctgtcgaaa aaatccagac tgggcgaagc gttcagctat gtactgaatc
      121 agtgggatgc cctctgttat tacagtgatg acggtctggc ggaggcggac aataacacag
      181 cagaaagagc gcttcgtacc gtctgtctcg ggaaaaagaa ttacatgttc ttcggcagcg
      241 atcatggagg cgatcgcgga gctctgctgt acggactaat cgggagctgt cggttgaacg
      301 gtatcgatcc agaagcgtat ctgcgccata tcctgagcgt actgccggaa tggccttcca
      361 accgagttga cgaactgctg ccatggaacg tagttctcac cgataaataa gcgtcaatac
      421 ggccttccgt tgacgcttac agaactcctg tggcgcgaga gtgaaaaact gcgtcgttat
      481 aaaaaggagg cccggttgcc agttgccaaa acgctaagcg aatacgactt cattcagttg
      541 ccggaactga atggagctca gttccagcaa ctctgtgaaa cgacagactg ggttgatgct
      601 ggagaaaacg ttctgctgtt cggggccagc gggttgggga aaagccatct ggcggcagcg
      661 atcgtggatg gcgtagtagg ccagggctac cgggcccggt tcaacagcgc aggagagctg
      721 ttgcaggaac tacataaagc cagagctcag ttgaaactga atgagctgct actgaaactg
      781 gatcgctacc gggtgatagt ggtggatgat cttggctatg tcaaacgcga cagcgccgaa
      841 acgggtgtgc tgttcgagtt aatagcgcat cgctatgaac gtgggagcct ggtgataacc
      901 agtaaccatc cgttcagcat gtggggcagc atcttcgtgg atgagactat ggcggtggcg
      961 gcagcagacc gactgatcca tcacggatat atgttcgaac tgaaagggga aagctacagg
     1021 aaaaagacag cgaaggcagt aacaagcgtg acttgatgtc gcactgaagg ggcggccagt
     1081 atagttggcg ccagtcggca aaactagttg acgtctaata cctggacggt cacttctgag
     1141 aaaaggcatt tacacagaat cttaaacagg ctcacggagt tctttcagaa aaaggcctgc
     1201 cgaatggcag gcctatccca ttcgtcagaa aacataacgg aagttggcgt ttatcgcatt
     1261 atccacatta tatttcccga acgctgattt ctccagctcc agtcccaaac gcatattatc
     1321 cttaatttcc gcattcatcc ctaccgtcat cagcatcctg ctatcttttt caccttcgaa
     1381 acgtttctct ccggaagcat cctgcagaac cgtttctccg ttcgccagca ggtcgaactg
     1441 ataacccagc ccggctcgag ctgtgatttt ccagtcgtct ccggagaagg ctcttcccac
     1501 gtcaacacca gtacggccaa tcagtgggtt ataatccttg tctttcatgc tcagagccat
     1561 tccccggtct tcccagctaa aagcttttcc tgatacagaa ccgtaaacca gctctatctg
     1621 tggctccacc caggactctt tcgtcaggtg gtaacgataa ccaacttcag caccggtata
     1681 ccaggaatga gagctgtagt cttttgttcc cagtgaggca aaattcgccg tgtgctgatt
     1741 atcatgatgg agatatttac caatcaggtc aaaataagct ccggaattaa agagtgcaga
     1801 ggcatacaga ccgccaccca cggatttgtt ttttccactg aatgcgtggc tgcttgcatt
     1861 gctgtccgta taggtcagca atgcccccgt aaataaatcc acaccgtcca gctcatgctt
     1921 tctgtcgaca ccaatctgaa cgtgagtgta gttatcgctg tagtcaccat ctgccgaacc
     1981 ggtaccattc attatgcgtg cccagacacc ggcatccccc tgagtatccc gcaggtcacc
     2041 catacgtttg ttcaggttat tgacctctgt aaggaaggtc ttataacctg atgccataaa
     2101 gtcttttgcg gctttcgagg ctccggcatc agcggtagtc tgatatcctg tcagcaccca
     2161 ttttgtggca tcatccgttt tttccgtgct gattactggc gtaacgttac tgaaaccaat
     2221 ctgctgcgta ccggcagtga acgttttctc atcactccct ttcggtgcgg taattaacgt
     2281 cacgctcatt tcactggctg gtttgtttaa aaagtcaacc aggacgctat tattactgcc
     2341 tgacagtttg tctgtgacat tcaactggtc agcccggctg ttgttggtat gcatcaggaa
     2401 cgtggtatta tctgcagaaa gctcttttac ttgtaaagtg gcaaagtgtt tcccgtcccc
     2461 cagttcaatt cgcgagccct tagccttcag tgcaccggcc tctgattttc cgtcagagta
     2521 ccacagagca ttgatcatat ttaccgtact ctgtgccccc gtaatactgc ctctcagaac
     2581 agcgttatat cccttcatta aggagctgaa taacggagag tccgtcttcc ctgcatcagc
     2641 atcactgtct cccagattaa tggttgccgc agtggttcct gcatgaatat ccgaactcag
     2701 atagcccata tttttaacag aaaagctggc gttatcttcg aggttaatcc cttctgttgt
     2761 agaaattacg ggtgagtaat acccctgttt ctgcgcagaa ggcatccccg tcagggtcag
     2821 agaagcattt tcttttacat ccacagcccc ggtgctgaaa ataccgctct gagccgtaag
     2881 ttttgctcct tttccgaggg tcaggctgct gttgacaaag ctgccaacac ggtcaaaaac
     2941 agcattctga gaggtcacac tcacggtgct atcataagcc tcaataccac cggtgaatct
     3001 ctccatgatt tgcagggatg atttattttc cagcgtaaca tttccgctgt aatcactctg
     3061 gtcaccaact gtggttgcag tggcactccc ttcaacagag gagaccgcat taccggttcc
     3121 gtcactcctg tcagtaaaga cacggctact tcccagaatg actatacttc cattggtggc
     3181 gttaatatct ccctgcatct tgccgtttct ggacagatgg aatgttgccg catcgagttt
     3241 cagtttttcc agcgtaaact gacgggtttc ccagtcgctc tggctggaag aagtcgtcgt
     3301 tccagcatga ataactggat gcccctggaa tatcagcgtg ccggacttct tggttatcga
     3361 accttctggc aggttaactg aaccatcaag agccagtaca tccttacctg agagctgcgg
     3421 aatattcaca tccatattgc cgtttagttg gccgtgatac atataaggct ggctgctggc
     3481 ctcaattttt tgttgcttca cagtatcaat ggctttatta cgatcttttc cgacattctg
     3541 ccagacactg ctgttgttca gatcggagaa gaacggggaa tatgaactcg ctttcaggat
     3601 gaaatattgc ttggtagaac tgtcataata taggtctccc ggcgctcctc tccccccaaa
     3661 aatactgaca gtattaaccg gtacattgat gtcactggct tttaatgtct gaaggtcgag
     3721 tgttaccgtt gattttttct cactgttatt ggtaatgatg gccccgtagt cagccgcctg
     3781 aagacgggta aaggtcagat tattaccatt cagttccaga cgtcctccac ggtatcccca
     3841 cgaaatatta tccggattaa cttgctgtga gtcagaaagc acaactgtcg gacgaccgct
     3901 ggcaatacca acagagctga aagcctgtac ctttccgtca gcatccgcct gctggttgag
     3961 aataacggta ccgtccccca ccttgagtcc gcctgcattc acaccggtcc cgtttactgt
     4021 cagggtacct tcgccagttt tatgcaggtt atcaccggca actccgttca cctgccacag
     4081 aacgtgggtt cctttatcgg tgataatccc gccccccatc catgtctggc cgttcagggc
     4141 ggacaccctg taatcgccgg caaactgcag atacccagct ccctgattaa cggaattctg
     4201 cagaacgacc tcgccattat tgccggtgaa caggaggttt tttcctgcgt tcagatcatt
     4261 tcctttcttt ccatgcatat cccaagtcgt gtttccctga gtcagtgtgc cggtgccatt
     4321 agccgcatca tatttccact gcagtactcc ttcaccagag gtgtaggcta tagttttgtc
     4381 aaaatcattt tgcggttgct gaccaaggaa atcctgagtg gtgacgaccc agttatttcc
     4441 atagttagtt cctgaggaaa gcacgccaat cagtacccat tttttttgca gggaatcata
     4501 agcaaacagg ggggagccgc tgtcccccat attcagataa ttagccagag gtccattcgc
     4561 gggattaaaa atgtcaccgg tctgagctgt aatcatctgt ccgttgttat aggagttcag
     4621 cgcgctgacg gttcccccaa caagaaatcc gccattggta tacacttggg tgcgattacc
     4681 atttttgtct ttgacgtact gcatcccccc cccaaggcga tagaattcag taaagcgtcc
     4741 gccggcctga taggcaccgc tcacagcccc gatgtcagac acttctgccg gcgccacctc
     4801 tgtcaccagt ttactcaggc ggcgggtttt aatatcaaga ccgctattat tatttgtacc
     4861 aaccgcagta taggtgtttt tggcgtatcc gaagctcatc gtgtcagaac cgctgacatg
     4921 tttggcggta accacatact gaggggagta aagtgtatgg tctcccggag gcaggctacc
     4981 ggtagtaata gtggcactac tgaaatcagc catcggggct ttatcaagtt taccgaccag
     5041 attgccctgc ttgtcgtaaa tggaaatatt cgtggtcccc gacgtaaact gacctttatt
     5101 ctcggcaaag tcgcggaata tctgataagg gatctctgca ctgaccgttg ctgacagagc
     5161 tggccatgcc gaagaaagtg ataaagctgc aacagacgta agaataaccc gacgtgaaag
     5221 tctgcgatgg cttttgcagg taaccctgcg ggccagttcg gagacagcaa tcaggctttt
     5281 ggttatatgg caatacttaa gataatatat tttgttcata tagaaacatc aacctaattt
     5341 tgacaaagat taattacata ggatgtagat ggagcagtcc gagattctac taatatcaat
     5401 tattaagcat ctgtttttta gttggtgtaa tataattttt attaaggtaa gcggaaaaac
     5461 aaccaaaaaa aacgcaagat gttatttttt tatcattttg agccagtttg gctgggggga
     5521 tgctcttctc ttaagcttct tgctcaactt taaaaatagt tatgccagtt cggtatcgat
     5581 aaggggcagt ttggatatc
//
LOCUS       DAETPZ010000171         5576 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.173, whole genome shotgun sequence.
ACCESSION   DAETPZ010000171 DAETPZ010000000
VERSION     DAETPZ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.173"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..87)
                     /locus_tag="K0W59_003984"
     CDS             complement(<1..87)
                     /locus_tag="K0W59_003984"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBI3024781.1"
                     /translation="MALESDERMLPPNIRGYAPQITGIAETNA"
     gene            complement(249..3557)
                     /gene="icsA"
                     /locus_tag="K0W59_003985"
                     /gene_synonym="virG"
     CDS             complement(249..3557)
                     /gene="icsA"
                     /locus_tag="K0W59_003985"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HBI3024782.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSSPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGEGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4086..5288
                     /gene="virA"
                     /locus_tag="K0W59_003986"
     CDS             4086..5288
                     /gene="virA"
                     /locus_tag="K0W59_003986"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HBI3024783.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1730 a         1256 c          935 g         1655 t
ORIGIN      
        1 tgcattagtt tctgcaatac cagtaatctg aggcgcataa ccacgaatat ttggcggtag
       61 cattcgttca tcgctttcta atgcaattcc tgtgtaacgc caagcaacag cgcattctgg
      121 cgcatgatct ggtaaacacg tttggcattg atcgcaggca taccatcatg tgcacaaaaa
      181 cccaccgagc ttttacacgg tgggtcccag agaaatgcag gacatcaaca cgccctgcat
      241 ttttattatc agaaggtata tttcacaccc aaaatacctt gggtatctct gtaactgtta
      301 ttacccaatt gctgagatac gtttccccag atgcttaggt tatctgcaag acgccccccc
      361 acaccagttt tgagttcaat cacattacgg ttgcctatct ggtgatttga catgccattc
      421 ataattacac catattgctt tgagctccat ttccaattca cttccaaaaa tggttcgaac
      481 ttacgaagag tctccttatc gatacaactc tgactaatta aggatgtacg caatcccaat
      541 tttgcttgaa tattattgac cccagaggat gtgaccatag tgccattgtg ttcaatatag
      601 tcgtttgctt ttaccccatg cctagttatc tggacttgtg gattgagata caatgcgttg
      661 tcaatattat tatgagcagt ccagcgtatg gttgggtaga tatagccagc ttccaaagct
      721 cctgttattc ctttgctgga atatttctca ccagttaacc catctccttt gactgtatta
      781 ttaaaccaac tgtactgcaa ccaagcatcc atatatagac ctgttcgttc tttttcatcc
      841 tgaaaccaag aactgtacaa accggcagta taaccgctca cagttcctcg tgaactataa
      901 ctagtatact tattatgagt tttgctatcc tgatatccat aagctcccat aatacccaca
      961 tgcaagccat catgatcagt gaagtttgtc ttcaaaatat ctccaccaat ctgataaacc
     1021 atcctgttag ctgtggtttt taattgtcta tcagctaaat tatgctcatt atgtccacca
     1081 acagtacgaa tccacatggt ggtttcgctg agctgacctg tagtaggatc aacaatagtg
     1141 ctaccgtgtc ggtcatagag agaaggacta aacatcgtgt tcattgctat caggttagca
     1201 atataactgc cagcttcagg gcgccatgtg tgaatactac cttcacccaa attaagtgaa
     1261 gatacaagct gtgaactagc tcggcgacta ctcatttgag tagactcttg attgctcatc
     1321 tgttttgatt cttgattatc catctgacta gttagatacc acttattggt attcagtcca
     1381 gatacagtgc cctgtttcag gcgatagtca taactaccag caacaatacg gcctttctga
     1441 ataaaagcat tcttatcaga agaatctgtc gaaataattt gaacaccttc aagagtttta
     1501 tctccagagc ctccctcatt tgtcactgca acataggtaa taccagacgc atggcctgca
     1561 atttccagcc ggtcagtttt actgttgtca ttgccgagaa ccgcgcccaa agataatatt
     1621 ccgccctctc catgccaatt acctttctga acataggtct gaccgacatt tgacgaacta
     1681 ttattgagaa taacatgacc gtttttctcc agagtcatgt tacctgtgac aatgacgggg
     1741 tcaggcaaaa aaggggcatt aatattattg attagcacag tcccactatt taataaagtg
     1801 ttaacagttt ggttcatgcc tgaaaaattc aaggtagcac ctttattaac aataacacca
     1861 gcggtacgtg tcatagcttc aactgtcccc atttttagaa tgcctgcatt gatgttggtg
     1921 aaaccagagt aggtattttt ttccgccaaa atgagagttc cctcccctga ttttgttagg
     1981 gattttccgt cccagctatt tatattatga tggctgagat tatctgccag aatagtacta
     2041 acagtaagtt cagcgttttc tttcagattg aactctccat gactgtcgcc atctgtatca
     2101 ttccagctca aaccgtagat caatttattc tggtcactga tatagctttt tgtatagtca
     2161 atagcaccag tagtgagtaa cggattgatg atagatatat tagaaacact gaaacctgtt
     2221 atccctttat ctgcacttag cagtttatag tccttctgcg cccagttatt tataatagct
     2281 tccactttta cattgttgcc aaatgacata tttccagaag cttccagact actgattcca
     2341 gctaaattta aagaactgtt ctcaccaaaa gaaacgtcac ctgaaacagt gaatttgtta
     2401 cctgatagtg aaacagttgt acaatcacca gcacataaat caccttcaat agttggtgtg
     2461 gtcccagtgt aatcattgtt gataattttc agaatggatg aattgttaac ctgtacatca
     2521 ccagtaatga cagaaccttc atggagtatt aatgaattgt ttccaccagt gatgtggata
     2581 gcattacctg ctacattttg tgttccatca tcttctttac ctgaaattat atatccgcta
     2641 ttagcaatgg tcatattgct accatttata gcatcccccc aatgtccacc attaccgcct
     2701 aaaatagctc cattgttgat tacagacagg ttatctcctg tgatagcatt accaccgtag
     2761 ccatcataac cataaccgta agcagcacct ccgttgcctc cagcgaacgt gcccttattg
     2821 attacagaga ggctgcttcc tgtgatggca tcacctccat taccgccatc accctcacca
     2881 taattgttac ctccattacc accggaaata gtccctttgt tgattatggg tagattgact
     2941 cctgtgacgg catcacctcc attcccccca tcactatcac tataactatc gccaccatgt
     3001 ccacctgaaa taatttctcc atttattata aacagattgt ctcctgtaac agcctcacca
     3061 ccatcaccgt tatggtcagc accgctaccg ccgctaccac caagaatcat gccttgattg
     3121 attatagaca agtcagatcc ggtaatagag tcaccaccat taccgccaca actattacca
     3181 tcattattat caccagcacc accatgacct gcgctaatca taatatcaga gttaatccgt
     3241 agcttagttc tagatgcatg agaggggctg gaagaagtta tataccacgc atccattcca
     3301 tcttcaccag ctcccaaggg agaaagtcca tcaacaggtg ttaataattt ttcataattg
     3361 tcctctgaaa aatgaagttc ttgagtaccc gaaagaggag tagcaaaagc tattggcccc
     3421 ccgagcaaca gggatgcacc aacaacaaaa ggagaaaaag acaatttgca tgttttttcc
     3481 tttaccgtag gtaattctcc ggcccccccc tgtgaacatt gggtcatatt acaaaaaaat
     3541 ttgtgaattt gattcatgca ctatattatc agtaagtggt tgataaaccc ctgaaaagat
     3601 tccgttttat ccggaataaa gggacgatat atgcaaaaac atattaaaca aagcctcaac
     3661 caaaacatga gagctgtgtt atatatcaat gcaacgggag aaatttacct gtgtcttgct
     3721 ttgaaaaaaa gagggggaaa gctatatgtt cgataataaa ttaataattt tactatgata
     3781 gtctgcttca gaccgcaact tttcagtcct ttctgtacgc ttgcccaaag tttaaccact
     3841 ttgtttttct atacacccaa tggttctatc tgccccttga tgttaatccc caaagccaat
     3901 aacatgaatg tattcatatc acaactttcc taccagactc tccaatgcaa taactgcata
     3961 attgttttta gtggaaatgt atcgttttct taaagagaag aataacattc cattttatta
     4021 tgttcccaat ttaattatat taatatatat tggcattaat aggaaaatac atcaggagaa
     4081 atcaaatgca gacatcaaac ataactaacc atgaaagaaa tgactcatca tggatgtcaa
     4141 ctgtcaaatc aactacagaa gtaagttgga ataaattgag tttttgtgac gttttactga
     4201 aaataataac tttcggcata tattctccac acgaaactct agccgaaaaa tatagcgaaa
     4261 aaaaactaat ggatagtttc tcaccatcac tatcacaaga taaaatggac ggagagtttg
     4321 cccacgcaaa catagatggc atttcaatac ggctatgcct taacaaaggc atatgttcgg
     4381 ttttttatct tgatggagat aaaatacaat ccactcaact ttcatcaaaa gaatataata
     4441 acttgttaag ttcactcccc cccaaacaat tcaacttagg aaaagtacat actattacag
     4501 ctccggtatc agggaatttt aaaactcaca agcctgcacc agaagttatt gaaacagcca
     4561 ttaattgctg tacatcgata ataccaaacg atgattactt ccacgttaaa gacactgatt
     4621 ttaatagtgt ttggcatgat atttacagag atattcgagc atctgacagc aatagtacaa
     4681 aaatatattt caacaatatt gaaataccac taaaattaat tgcagatcta attaatgagt
     4741 taggaattaa tgaattcatt gatagcaaaa aagagcttca aatgctaagt tacaaccaag
     4801 taaataaaat aatcaattca aacttccccc aacaagacct atgttttcaa acagaaaaac
     4861 ttttgtttac ttcactcttt caagaccctg catttatatc tgcactaaca tctgcattct
     4921 ggcaatctct tcacatcacg tcttcctctg tggaacacat atatgcccaa attatgtccg
     4981 aaaacataga aaacaggctt aattttatgc ctgaacaacg agttattaac aattgtggac
     5041 atattataaa aatcaacgca gttgtcccta aaaacgacac tgcaatctct gcctctgggg
     5101 ggagggctta cgaagttagc tcatcaatat taccatcgca cataacatgt aatggtgttg
     5161 gtattaataa aatagaaaca agctatctcg ttcatgctgg aacacttcca agcagtgaag
     5221 ggcttcgcaa tgctatccct ccagaaagtc gtcaagtatc atttgccatc atatctcctg
     5281 atgtttaagc caatattata ttggctgcca gactgtaaaa aaacgttctt cagaacagct
     5341 ctataaacta ttaaaccaaa gctaaaagga tggggaccaa tagaatctca gcccatcctt
     5401 tttatatcat tatgaaccgc cccggaaatc ctggagacta aactccctga gaaagaggta
     5461 aacaggatga ctaaaaatac acgtttttcc cctgaagtcc gtcaacgggc agttcgtatg
     5521 gttctggaaa gtcagggcga atataactca caatgggcag cgatttgttc cattgc
//
LOCUS       DAETPZ010000172         5533 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.174, whole genome shotgun sequence.
ACCESSION   DAETPZ010000172 DAETPZ010000000
VERSION     DAETPZ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.174"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            100..1008
                     /locus_tag="K0W59_003987"
     CDS             100..1008
                     /locus_tag="K0W59_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HBI3024784.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1054..3045)
                     /gene="tkt"
                     /locus_tag="K0W59_003988"
     CDS             complement(1054..3045)
                     /gene="tkt"
                     /locus_tag="K0W59_003988"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBI3024785.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3323..4081
                     /gene="loiP"
                     /locus_tag="K0W59_003989"
                     /gene_synonym="yggG"
     CDS             3323..4081
                     /gene="loiP"
                     /locus_tag="K0W59_003989"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HBI3024786.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4287..5207)
                     /gene="speB"
                     /locus_tag="K0W59_003990"
     CDS             complement(4287..5207)
                     /gene="speB"
                     /locus_tag="K0W59_003990"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBI3024787.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5343..>5533)
                     /locus_tag="K0W59_003991"
     CDS             complement(5343..>5533)
                     /locus_tag="K0W59_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024788.1"
                     /translation="FQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNALPPCS
                     GQAFPADNNGYAPPPCRK"
BASE COUNT         1324 a         1484 c         1479 g         1246 t
ORIGIN      
        1 ttcgctatac ccctttatag attctgaatt ctcacctatc ggctgttttc tcctcatctc
       61 atgtgacata ttcccatact ttactcaagg aagattgtca tgaaagcacc tgtttcatta
      121 acctcatttc gcccacaaaa atctcttgct atcgccattg gcgtgcttgc cgtggttgtg
      181 ctgccatttc tctcgtatta caccgttaat gaaggtgaac gcggcatttt gctgcgctac
      241 ggaaaaatcg taaaagttgc cgagccgggg ctgggtttta agatcccttt tatggaaagc
      301 gtggagaaaa tttccacgcg taatcaggct gtggtctatc agggattaca ggcctacagc
      361 cgcgaccagc aaccggcaca aatgacagta tcagtcagct tccacattaa accttcagaa
      421 gcaggcgcgg tttataccac ctacaacact attgaagcat taaaagatcg tctaattgtc
      481 cgtcagttgc caactcagct ggagaacatt ttcggccaat ataccgccat cagcgccgta
      541 caggatcgta caaaattggt tcaggatctg caaaatgcca tgcgcaaagc cgttgttggg
      601 ccagtggtaa ttgacggggt ccagattgaa aatattgatt tttctgacgc ttatgaaaag
      661 tcgattgaaa accgaatgaa agcagaagtc gccattgcca cgcgtaagca aaatctggaa
      721 acagagaaaa tccaggcgca aatagcggta actcaggccc aggcagaagc agacagtaaa
      781 ctggcggcag ccaaagcaga agcggaaact attcgcgtaa gaggcgcggc agaggctgaa
      841 actattcgtt taaaaagtgc ggctgaagca gaagcaatcc gcctgcgcgg tgaagctctg
      901 cgggataatc ccggacttgt agccctcacc actgccgaac gttgggacgg taaactgccc
      961 gataccatga tccccggcag cacagttccg tttatctcta ctaagtaaaa aaagggccgc
     1021 tttcgcggcc cttctcacaa aacgtcttac tgattacagc agcgcttttg ctttcgcaac
     1081 aacgttatca acagtgaagc cgaactcttc aaacagcagc tctgccggag cagattcacc
     1141 gaaggtggtc atgccgacga tagcgccgtt caggccaaca tacttgtacc agtagtcagc
     1201 aatacccgct tctaccgcaa cgcgtgcagt aaccgctttc ggcagtacgg attcacggta
     1261 agccgcatcc tgcttgtcaa atgcgtcggt agacggcatg gacaccacgc gcgctttcac
     1321 gccttcggca gtcagttttt cgtaggcagc aaccgccagt tcaacttcag aaccggtggc
     1381 gatgaagatc agttccggct gaccagcaca gtctttcagc acataaccac cgcgcgcgat
     1441 gtttgccagc tgctcttcag tacgttcctg ctgcgccagg ttctgacggg agaggataag
     1501 cgcagtcggg ccgtcctgac gctcaacgcc gtatttccac gcgaccgcgg attcaacctg
     1561 gtcacacgga cgccatgtag acatgttcgg ggtcacgcgc agagaagcga cctgctctac
     1621 cggctggtga gtcgggccat cttcgcccag accgatggag tcgtgggtgt aaaccatcac
     1681 ctggcgctgt ttcatcagcg cagccatacg tacggcgtta cgtgcgtatt ccacgaacat
     1741 caggaaggtg gaggtgtacg gcaggaagcc accgtgcagg gagataccgt tagcaatcgc
     1801 ggtcataccg aactcgcgaa caccgtagtg gatgtagtta cctgcagcat cttcgttgat
     1861 tgctttagaa ccagaccaca gggtcaggtt agacggtgcc aggtcagcag agccgccgag
     1921 gaattcaggc aacagcgggc cgaacgcttc gatagcattc tgcgacgctt tacggctggc
     1981 gattttcgcc ggattagcct gcagtttagc gataaactct ttcgctttgg cgtcgaagtc
     2041 agacggcatt tcgcctttca tacggcgggt aaattcagcc gcttcctgcg gataagcttt
     2101 cgcgtaagcc gcaaacttct cattccatgc agattctttc gcctggcctg cttctttcgc
     2161 atcccactga gcatagattt cagacgggat ttcgaacggt gcatatttcc agcccagctg
     2221 ttcgcgggtc agggcaattt cagcgtcgcc cagcggcgca ccgtgggagt cgtgagtacc
     2281 ggctttgttc ggggaaccga aaccgatgat ggttttgcac atcagcaggg acggtttgtc
     2341 agtcactgcg cgcgcttctt caactgcgcg tttgatggat gccgcgtcat gaccgtcgat
     2401 gtcgcgaata acgtgccagc cgtaagcttc gaaacgcatt gctgtgtcgt cggtgaacca
     2461 gccttcaacg tgaccatcga tagaaatacc gttgtcatcg tagaacgcaa tcagtttacc
     2521 cagcttcagc gtacccgcca gagagcaaac ttcgtgggag atgccttcca tcatgcagcc
     2581 gtcgcccatg aaggcgtagg tgtagtggtc gacaatgtcg tggcccggac ggttaaactg
     2641 cgccgccagc gttttttctg caatcgccat accgactgcg ttggcaatac cctgacccag
     2701 cggaccggtg gtggtttcca caccagcggt ataacctact tccgggtggc ccggagtttt
     2761 agagtgcagc tgacggaagt ttttcagttc ttccatcggc agatcgtaac cggtgaggtg
     2821 cagcaggctg tagatcagca tggagccgtg gccgttggac agcacgaagc ggtcacggtc
     2881 agcccaggac ggattctgcg ggttgtgttt caggaaatca cgccacagga cttcggcaat
     2941 gtcagccata cccatagggg cacccgggtg accggatttg gctttctgta ctgcgtccat
     3001 gctcagcgca cgaatagcat tggcaagctc tttacgtgag gacattttga ctccagatcg
     3061 gatgatgaag ggcacgccct taacgacttg acgacagcgc gttttgggct acgccggaaa
     3121 atttgccaac aatttaccgc aagccgcgcg tcatgtacat ggaacatcct tttgccgctt
     3181 cagaaatctc tggatcatgc tcgcatgttg cgcaatctgc tcgcccgtcc gctgcgcttt
     3241 tccttatact gagactgagc gtcgattcac ctgcaaacgg cgcattttta gaataatcct
     3301 gaccttgtgc ggaagagaaa acatgaaaat tcgcgcctta ttggtagcaa tgagcgtggc
     3361 aacggtactg accggttgcc agaatatgga ctccaacgga ctgctctcat caggagcgga
     3421 agcttttcag gcttacagtt tgagtgatgc gcaggtgaaa accctgagcg atcaggcatg
     3481 tcaggagatg gacagcaagg cgacgattgc gccagccaat agcgaatacg ctaaacgtct
     3541 gacaactatt gccaatgcgc taggcaacaa tatcaacggt cagccggtga attacaaagt
     3601 gtatatggcg aaggatgtga acgcctttgc aatggctaac ggctgtatcc gcgtctatag
     3661 cgggctgatg gatatgatga cagataacga agtcgaagcg gtgatcggtc acgagatggg
     3721 gcacgtggcg ttaggccatg tgaagaaagg gatgcaggtg gcgcttggta caaatgccgt
     3781 gcgagtagct gcggcctctg cgggcgggat tgtcggcagt ttatctcaat cacaacttgg
     3841 tgatctgggc gagaaattag tcaattcgca attctcccag cgccaggaag cagaagccga
     3901 tgattattct tacgatcttc tgcaccaacg cggcatcagc ccggcaggtc ttgccaccag
     3961 ctttgaaaaa ctggcgaagc tggaagaagg ccgtcaaagc tcaatgtttg acgaccatcc
     4021 tgcatccgcc gaacgtgccc agcatattcg cgatcgcatc agcgcggatg ggattaagta
     4081 aagcctggtg gtgtcgaacg taggtcagat aaggcgttca cgccgcatct gacattaatg
     4141 gcacatttgt taccttgtgc gcattgccgg atgcgatgct ggcgcatctt atccggccta
     4201 cgggtgccga acgtaggtcg gataaggcgt tcacgccgca tccgacatta atggcacgtt
     4261 ttacccgtgc gcatcgcatc tggtgcttac tcgccctttt tcgccgcctg aatatacagc
     4321 atctccagcg ccagcgtcgc cgcagccaga gcggtgattt ccgactgatc gtatgccgga
     4381 gccacttcca ctacgtccat cccaacgatg ttgagatctt tcaggccgcg taccagttta
     4441 atggcgcgat cggaggtcag gccgccaatc actggcgtac cggttcccgg cgcgaaagca
     4501 ggatccaggc agtcgatatc aaaagtcagg taaaccggca tatcacccac aatctgtttc
     4561 acctgggcga taacgtcatc cacgctgcga tcgttcacct ggcaggcgtc cagcacggta
     4621 aagccgttgt ctttatcaaa ctcggtacga ataccaatct gcacggaatg attcgggtcg
     4681 atcagacctt ctttcggcgc ggtatagaac atagtgccgt ggtcaaattc acaaccgttc
     4741 gcataggtat cggtgtgggc gtcaaagtgt accagcgcca ttttgccgaa atgcttcgca
     4801 tgagcacgca gcagcggcag cgtaacaaag tggtcaccac cgaaggagag catacgctta
     4861 ccggcagcca gcagcttctc ggcgtgcgcc tgcagctttt cgctcatctc acgggcatcg
     4921 ccaaaggcat ataccagatc gccgcagtcc acgacgttca gacgctcacg catgtcgaaa
     4981 ttccacggga agcggttgtg ttcccaggcc agattcgtcg aaacctgacg gatcgctgcc
     5041 ggaccgtggc gaccacccgc acgaccagaa gtggccatat cgaacggcac gccagtaatc
     5101 acccagtctg catcgctgtc atacggctgg aagttcatcg gcaggcgtaa aaaaccaaag
     5161 gcattggaaa ccagtgagtt atcgtattga tgacctaagg tgctcatgga tggactccta
     5221 ttacaaagtc gatataaaaa aacccttccg cgtcgttagg cccgacgagg aagggttgga
     5281 tttgcgaaac acaagtttgt gctaattatc gccttaaatg ggcgaggcat caagcgaggt
     5341 tgctatttcc gacacggtgg cggcgcataa ccattgttat ctgccggaaa ggcttgtcca
     5401 gagcacggcg gcagcgcgtt ttgcgccggg ccttgtaatt caaaggcggc aaagccgatt
     5461 accccggtat tacgcacatc gccttgcgcc gaattggctg cgtaggaatc agcaacgttg
     5521 gcgaactgaa aag
//
LOCUS       DAETPZ010000173         5500 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.175, whole genome shotgun sequence.
ACCESSION   DAETPZ010000173 DAETPZ010000000
VERSION     DAETPZ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.175"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..144
                     /locus_tag="K0W59_003992"
     CDS             <1..144
                     /locus_tag="K0W59_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBI3024789.1"
                     /translation="IPELMRDLASMGQLVKLPTRRGRAFPRVVKERPWKYPTAPKKSQ
                     SVA"
     gene            complement(195..932)
                     /gene="bamD"
                     /locus_tag="K0W59_003993"
     CDS             complement(195..932)
                     /gene="bamD"
                     /locus_tag="K0W59_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HBI3024790.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            1067..2047
                     /gene="rluD"
                     /locus_tag="K0W59_003994"
     CDS             1067..2047
                     /gene="rluD"
                     /locus_tag="K0W59_003994"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HBI3024791.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINSEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            2044..2775
                     /gene="pgeF"
                     /locus_tag="K0W59_003995"
     CDS             2044..2775
                     /gene="pgeF"
                     /locus_tag="K0W59_003995"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HBI3024792.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2905..5478
                     /gene="clpB"
                     /locus_tag="K0W59_003996"
     CDS             2905..5478
                     /gene="clpB"
                     /locus_tag="K0W59_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBI3024793.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEINEDRIVAV
                     Q"
BASE COUNT         1357 a         1327 c         1504 g         1312 t
ORIGIN      
        1 ataccggagc tgatgcgcga tcttgcaagt atgggacaac ttgtgaaatt accgacgaga
       61 aggggaaggg ccttcccgag agtggtaaag gagaggccct ggaaataccc cacagccccg
      121 aaaaagagcc agtcagttgc ttaactgact ggcattacag ctcaagggct gccgttttgt
      181 gtttcaggtt tctgttatgt attgctgctg tttgcggcga tgattttcgc tactttttca
      241 gcttgcgcat tcatctgcat ctgacggtat gcattttcca tcagcggcag cgcatcacgc
      301 gtagcctggg tatccgggta gtcgcgcaac atgccttcta cgcggttaac gacggcaacc
      361 catgcgccac gttctgtata gtactcggcc acggagtatt catatttcgc cagacgatct
      421 ttcaggaata ccagacgttt ggtggcatcg gtagtgtact gactgttcgg atagccgcgc
      481 accagtttgg aaaagtcact aaacgcagct cgtgcatgtt gaggatcgcg atcgctacgg
      541 tcaacgccaa agaacccttg cagcgcactg tcatccagcg ccatattggt caggccacgc
      601 atgtacatga cataatcgat attcggatgg gtcgggttaa ggcgaataaa acgatcgatg
      661 gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg cgtagatgag atccagctgc
      721 acctgctgcg aatacggacc aaacggatag cgattatcta acgcttccag ttgcgttatt
      781 gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag tcgcgtaaat ttcatttggc
      841 ggattatcag gtacttcttc ctttgacccc gagcaacccg ccaaaaacag gcttagtgtg
      901 gcggctgcca ccagatattt catgcgcgtc atgacgtttt gactttcctc aaaatgtaat
      961 acgggagatt ctctgttcct gctcccggtt aagaccagct acaatagcac actatattaa
     1021 acggcaaagc cgtaaaaccc caacgataaa cgaagaagca gtatatatgg cacaacgagt
     1081 acagctcact gcaacggtgt ccgaaaacca actcggtcaa cgcttagatc aggctttggc
     1141 cgaaatgttc ccggattatt cacgttcgcg aataaaagaa tggatcctcg accagcgcgt
     1201 gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa gtattgggtg gcgagcaggt
     1261 tgccatcaac tctgagattg aagaagaagc gcgttttgaa ccgcaggata tccctctgga
     1321 tatcgtctat gaagatgaag atatcatcgt cattaataaa ccgcgcgacc tagtggtaca
     1381 tcctggcgcg ggtaacccgg atggcacggt actgaatgcg ttgcttcatt actatccgcc
     1441 cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg gataaagaca ccactggcct
     1501 gatggttgtg gcaaaaaccg ttccggctca gacgcgttta gtcgaatctt tgcaacggcg
     1561 tgaaattact cgtgagtatg aagcggtggc gattggtcat atgaccgctg gcggtacagt
     1621 agatgaaccc atcagccgcc acccgaccaa acgtactcac atggcggtgc atccgatggg
     1681 caaaccggcg gtgactcact atcgcatcat ggaacacttc cgtgtgcaca cgcgtctgcg
     1741 gttgcgtctg gaaactggac gtacgcacca gatccgcgtg catatggccc atatcactca
     1801 tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt ccgccaaaag gtgcttcgga
     1861 agcatttatc tccacgctgc gtaagtttga ccgccaggcg cttcatgcaa ccatgctgcg
     1921 tctttatcac ccgatctccg gcatcgaaat ggaatggcat gcgcctattc cacaagatat
     1981 ggtggagctg attgaggtga tgcgcgccga tttcgaagag cataaggatg aagtggactg
     2041 gttatgagta agctgattgt cccgcagtgg ccgctgccaa aaggtgttgc ggcctgtagc
     2101 tccactcgta tcggcggcgt gagcttgccc ccgtatgact cactcaacct cggtgcccat
     2161 tgtggcgata acccggatca cgttgaggag aatcgcaagc gactttttgc tgcgggcaat
     2221 ttgccttcta aaccggtctg gcttgagcag gtacacggca aagatgtgct taagcttact
     2281 ggcgaacctt atgcctcaaa acgagcagat gcctcttata gcaatacgcc tggcacggtt
     2341 tgcgcagtga tgactgccga ctgcctccct gtgctgtttt gcaatcgagc gggaacggaa
     2401 gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag gcgtgctgga agagacggtt
     2461 tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt tagggccggc aattggtcca
     2521 cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta tggcagtaga cgctaaagca
     2581 agtgcagctt tcattcagca tggtgataag tatctggcgg atatttatca gcttgcccgg
     2641 cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg gcgaccgttg tacatatacg
     2701 gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca ccggtcgtat ggcaagtttc
     2761 atttggctga tataacctaa agaatcaaga cgatccggta cgcgtgattt tcttttcaca
     2821 ttaatctggt caataacctt gaataattga gggatgacct catttaatct ccagtagcaa
     2881 ctttgatccg ttatgggagg agttatgcgt ctggatcgtc ttactaataa attccagctt
     2941 gctcttgccg atgcccaatc acttgcactc gggcacgaca accaattcat cgaaccactt
     3001 catttaatga gcgccctgct gaatcaggaa gggggttcgg ttagtccttt attaacatcc
     3061 gctggcataa atgctggcca gttgcgcaca gatatcaatc aggcattaaa tcgtttaccg
     3121 caggttgaag gtactggtgg tgatgtccag ccatcacagg atctggtgcg cgttcttaat
     3181 ctttgcgaca agctggcgca aaaacgtggt gataacttta tctcgtcaga actgttcgtt
     3241 ctggcggcac ttgagtctcg cggcacgctg gccgacatcc tgaaagcagc aggggcgacc
     3301 accgccaaca ttactcaagc gattgaacaa atgcgtggag gtgaaagcgt gaacgatcaa
     3361 ggtgctgaag accaacgtca ggctttgaaa aaatatacca tcgaccttac cgaacgagcc
     3421 gaacagggca aactcgatcc ggtgattggt cgtgatgaag aaattcgccg taccattcag
     3481 gtgctgcaac gtcgtactaa aaataacccg gtactgattg gtgaacccgg cgtcggtaaa
     3541 actgccatcg ttgaaggtct ggcgcagcgt attatcaacg gtgaagtgcc ggaaggattg
     3601 aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg tggctggggc gaaatatcgc
     3661 ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc ttgccaaaca ggaaggcaac
     3721 gtcatcctgt ttatcgacga attacatacc atggtcggcg cgggtaaagc cgatggcgca
     3781 atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg gtgaattgca ctgcgtaggt
     3841 gccacgacgc ttgacgaata tcgccagtac atcgaaaaag atgctgcgct ggaacgtcgt
     3901 ttccagaaag tgtttgttgc cgagccttct gttgaagata ccattgcgat tctgcgtggc
     3961 ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta ctgacccggc aattgttgca
     4021 gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc tgccggataa agccatcgac
     4081 ctgatcgatg aagcagcatc cagcattcgt atgcagattg actcaaaacc agaagaactc
     4141 gaccgactcg atcgtcgtat catccagctc aaactggaac aacaggcgtt aatgaaagag
     4201 tctgatgaag ccagtaaaaa acgcctggat atgctcaacg aagaactgag cgacaaagaa
     4261 cgtcagtact ccgagttaga agaagagtgg aaagcagaga aggcatcgct ttctggtacg
     4321 cagaccatta aagcggaact ggaacaggcg aaaatcgcta ttgaacaggc tcgccgtgtg
     4381 ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa tcccggaact ggaaaagcaa
     4441 ctggaagccg caacgcagct cgaaggcaaa actatgcgtc tgttgcgtaa taaagtgacc
     4501 gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga ttccggtttc tcgcatgatg
     4561 gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac tgcaccatcg cgtcattggt
     4621 cagaacgaag cggttgatgc ggtatctaac gctattcgcc gtagccgtgc ggggttggcg
     4681 gatccaaatc gcccgattgg ttcattcctg ttcctcggcc caacgggtgt ggggaaaact
     4741 gagctttgta aggcgctggc gaactttatg tttgatagcg acgaggcgat ggtccgtatc
     4801 gatatgtccg aatttatgga gaaacactcg gtgtctcgtc tggttggtgc gcctccggga
     4861 tatgtcggtt atgaagaagg tggctacctg accgaagctg tgcgtcgtcg tccgtattcc
     4921 gtcatcctgc tggatgaagt ggaaaaagcg catccggatg tcttcaatat tctgttgcag
     4981 gtactggatg atgggcgtct gactgacggg caggggagaa cggtcgactt ccgtaatacg
     5041 gttgtcatta tgacctctaa cctcggttcc gatctgattc aggaacgctt cggtgaactg
     5101 gattatgcgc acatgaaaga gctggtgctc ggtgtggtta gccataactt ccgtccggaa
     5161 ttcattaacc gtattgatga agtggtggtc ttccatccgc tgggtgaaca gcacattgcc
     5221 tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc tggaagaacg tggttatgaa
     5281 atccacattt ctgacgaggc gctgaaactg ctgagcgaga acggttacga tccagtctat
     5341 ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg aaaacccgct ggcacagcaa
     5401 atactgtctg gtgaattggt tccgggtaaa gtgattcgcc tggaaattaa tgaagaccgg
     5461 attgtcgctg tccagtaatg ataaaacgag cccttcgggg
//
LOCUS       DAETPZ010000174         5392 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.176, whole genome shotgun sequence.
ACCESSION   DAETPZ010000174 DAETPZ010000000
VERSION     DAETPZ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.176"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..446)
                     /locus_tag="K0W59_003997"
     CDS             complement(<1..446)
                     /locus_tag="K0W59_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HBI3024794.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(501..1352)
                     /gene="manZ"
                     /locus_tag="K0W59_003998"
     CDS             complement(501..1352)
                     /gene="manZ"
                     /locus_tag="K0W59_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HBI3024795.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALSAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1365..2165)
                     /gene="manY"
                     /locus_tag="K0W59_003999"
     CDS             complement(1365..2165)
                     /gene="manY"
                     /locus_tag="K0W59_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBI3024796.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2228..3199)
                     /gene="manX"
                     /locus_tag="K0W59_004000"
     CDS             complement(2228..3199)
                     /gene="manX"
                     /locus_tag="K0W59_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBI3024797.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3662..5212
                     /gene="yoaE"
                     /locus_tag="K0W59_004001"
     CDS             3662..5212
                     /gene="yoaE"
                     /locus_tag="K0W59_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HBI3024798.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFFFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5216..5389)
                     /locus_tag="K0W59_004002"
     CDS             complement(5216..5389)
                     /locus_tag="K0W59_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rtn protein"
                     /protein_id="HBI3024799.1"
                     /translation="METQEQAKYLRRHGVHILQGYLYAQPMPLRDFPKWLAGSQPPPA
                     RHNGHITPIMSLR"
BASE COUNT         1311 a         1362 c         1373 g         1346 t
ORIGIN      
        1 acgagaagct tataaatttt ttccagatcg tcgatatttc gaacgcggat taacagccga
       61 cgctgttcta attgcatcac caacacgcca tcttccgaca agttcatcgc tttgattcgg
      121 ctatattcaa tccagacatt ggcgaagaaa aaaccttttt gtttaaagat gatcttcgga
      181 atgcggatcc agaatatata aaaacccatc agagccagtg cacttaataa ccatgttgtt
      241 attagcgcgc catgattcgt gacgttgtta tagataagaa tgacaatcaa tccgacgaag
      301 ataacgctat cgatgcgacc acgccggagc aaaggaattg ccagcagggt ggggccgtta
      361 cggcggggca tgatgaactg atcgtagatc gcgaaggcca ggagtgcggc gatgaaaaga
      421 atcagtacca ggtccgtgat tgtcattaat cctccagata aaaaaacggg gccaaaaggc
      481 cccggtagtg tacaacagtc ttacagtccc agcaggccgc aagcgtaacc agcgataccg
      541 atgacgaaga agccaacgat gatccacagc gggttaactt ttttgcgcag cagccacata
      601 caagcaaatg tcagcagcag tggtacaagg cctggcatta actggtcaag aatagtttgc
      661 acggtggtga cacgggtctg tccatcctgc ccggtgatgg tcgaaaccac cagcgggatg
      721 ttgacatgtg tccacttgtt aaccaatgcc cccatgacaa acaggccgag gatagacgcc
      781 ccttccgtca gtttttgcag gaagccacca cccatatctt taacgatatc gatacctttg
      841 gagtaaccat acgctacgcc gtagtaacgg gttgccagac gcaccaggtt aaacaggatg
      901 aagaacagca gcggacctaa caggctgccg ctcatcgcga taccggcact cagtgctgca
      961 aataccggac gtacggttcc ccagaagatc gggtcgccta caccagccag cggccccatc
     1021 aaaccgactt tgataccgtt gatagcaccg tcatcgatct ctgcgccatt agcacgctgt
     1081 tcttccagcg ccagggttac gccgagaatc ggtgcagcca cgaacggctg ggtgttaaag
     1141 aactccaggt gacggcgaat agcttgttta cgagcttcgt tgttctcagg gtagaggcga
     1201 cgaattgccg gtaccataga gaagcagaaa cccagtgcct gcatacgttc gaagttccat
     1261 gaaccctgga agaggttaga acgcaggaat acgccacgaa tatcactttg agtgagtttt
     1321 ttctcggtgg tagtttgagt tgtatcaacc atttcgctca cctgttagtc cagttcgtta
     1381 tcgagatcgt tgttaccagc tgcctgagca ggcgcaccgg ctacgcggtt gtatttcggg
     1441 ctaagttgga tgtagagcac tgccataaca gtaccaatca cacccagagc aaccaggtta
     1501 aagttggtga atgctgcggt tacgaagccg aggtagaaga acggcatcag gtagccagca
     1561 cgcatcatgt tgataaccat cgcataacca accaccacga tcatgccccc ggcgatattc
     1621 agaccattgg tcaccacttc cggaatcgca ttcagcatgt tctgtacttc gctggtacca
     1681 acagacagcg caacgatgac ggccggaata gccacacgca ttgcttgcag gaacagagaa
     1741 gaaacgtgga tccaggaaat cgctgtcagg ttgccgttat cagcagcctt atccgcagcg
     1801 tgctggaaag caacggtaat agtacgaacg atgatggtca gtacctggcc cgcagcggcc
     1861 agagggattg ccagtgcgat acctgcacca atgctctgat gacctgcgat aaccagaatg
     1921 gtagaaatga tagaagccag agcggcgtca ggcgcaactg cagcaccgat gttcatccag
     1981 cccagcgcga tcatttccag cgtaccaccg ataataatac cggttttcat atccccaaga
     2041 acgataccca ccagggtaca cgcgattagc ggacggtgaa actgaaattc atcgaggatt
     2101 gatcccatac ctgcgataca ggctacgata aataccagca caatttgaag agtggtaatc
     2161 tccattgtac ttctcctgtt tacgtaagac ttaagtgtga aaactgaatg ataatcaaca
     2221 caatacgtta cttatcgatt ttgctgatca gatccatcat tttcagtttc ggatcggtgg
     2281 aaaccttacg gacttccagc tcaataccgc gtgcattcag ttttttgaac gcctcgatat
     2341 ctttttcatc aaccgaaacc gcgttattca cctgggtttt accctgacgg aatgccatac
     2401 caccgacgtt aacagaggtg attttcacgc cgccttcaac gagacgctct acatctgttg
     2461 ggttggtaaa taacagcatt acgcgttcgc cagcatattt cgggttgttg tagacgcgaa
     2521 tcattttggc aacatcaact acgtgtgctg ttacgcccgg aggtgcaacc tgggtgagca
     2581 gtgtcttacg aacggtatcc gcagccactt catcactaac aacaataata cgggagacat
     2641 tggtttcttt ggtccagcgg gtggcgacct gaccgtgaat cagacggtcg tcgatacgcg
     2701 caaggccaat aaccatgtag tcgtttggcc ccattggttt tgccggagtt ggagccgctt
     2761 ttggtgctgc ggcagcgggt gctggcgcgg ctttttcaac cggtttggct ttcagtgctt
     2821 tcacgccttc acggcctgtt tctactgcca gcgcaaccag ttcatcaaag ctcgggtcat
     2881 catcacgggc cattaacgtt tccacgagca ttggaatgtt aacgcctgca atgacttcat
     2941 aatgctcttt gtcgacgaca atgcggctgg cagcattgaa cgggctgcct ccccatgtat
     3001 caacgagaaa cagcacgcct ttagtggtgt cgagttttgc caactgagcg ttgtattttt
     3061 caatcagcgt ttcggcattt tcacctggaa cgaaatcgat ccagccgacg ttttcctgct
     3121 cgcctaacag catttctgcc gttttaagca actgctctgc agcccaacca tgtgtgccta
     3181 taacaatagc aatggtcact tgctacctcc tttattatcg ttaacacctc aacgtgccag
     3241 atgtattttt gaatcgtcgt ccacaatcga atcgattcag ataagggcta caaccaaaaa
     3301 cccctgcgtt tcgcgattta ttttagatat cgaaaaaatt attttatgtg atgaagatcc
     3361 gtaatttaac tttcgattat cagaaatttc gaaaggtaaa atatccttgt cacattcgtt
     3421 tgcaaaggaa ggtaaatctt tgccaaatca gaggcgtctc tgatatgttt aactcccgtt
     3481 taacaaccat ggagtatagg gcagtagccc gcagtatgga tcgtcaccga cgtcatttca
     3541 gcatcaggcc ttttaacgcc tgcctttctg gcactctatg ccgcaccttt cgtttgcatt
     3601 ttgtcgttac gcctgcatta tttctggcgt cgaatagcta ttccttaagc aggagcttgt
     3661 catggaattc ttaatggacc cctcaatttg ggcggggcta ctcacgcttg ttgttctcga
     3721 aattgtgctg ggtatcgata acctggtctt catcgccatt cttgctgaca aactgccgcc
     3781 aaaacaacgc gataaagcgc gtttgctggg gttatcactg gcgctgatta tgcgtctggg
     3841 gctgctgtcg ctgatttcat ggatggtcac gctgaccaaa ccgctattta ccgtcatgga
     3901 tttcttcttc tccggacgcg atctgattat gttgttcggg gggatattct tgctgttcaa
     3961 agcaacaacc gaactgcatg aacggctgga aaaccgcgat catgattccg gccacggtaa
     4021 aggctacgcc agtttctggg tggtcgtcac acagattgtc atccttgacg ccgtcttctc
     4081 gttggatgcg gtaattactg cagtagggat ggttaaccat ctgccggtga tgatggcggc
     4141 ggtagtgatt gcgatggcgg ttatgttgct ggcatcgaaa ccgctgacgc gattcgttaa
     4201 ccagcacccc acggtggtgg tgctctgtct gagcttcctg ttaatgattg gtctgagtct
     4261 ggtggcagaa ggtttcggtt tccacattcc gaaaggttac ctgtatgccg cgattggctt
     4321 ctcgatcatt atcgaagtgt ttaaccagat tgcgcgtcgc aactttattc gtcaccagtc
     4381 gactttgccg ctgcgagcgc gtactgccga tgccatcctg cgtttgatgg gcgggaaacg
     4441 tcaggccaat gttcagcacg atgccgataa cccgatgccg atcccggaag gtgcatttgc
     4501 cgaagaagaa cggtacatga ttaacggcgt actgacgctg gcgtcgcgtt ctctgcgcgg
     4561 gatcatgacg ccgcgcggtg aaataagctg ggttgacgct aatctcgggg tcgatgaaat
     4621 ccgcgagcaa ctgctctctt caccgcacag tctgttcccg gtatgtcgcg gtgaactgga
     4681 tgaaatcatc ggtatcgtac gtgctaaaga actgctggtg gcgctggaag agggcgttga
     4741 tgtggcggcg attgcttcgg cgtctccggc gattatcgtc ccggaaaccc tcgatccgat
     4801 caacctgctg ggcgtgctgc gtcgcgctcg cgggagcttt gttatcgtga ccaacgagtt
     4861 tggtgtggta caaggcttgg tcacgccgct ggatgtgctg gaagccattg cgggtgaatt
     4921 cccggacgct gacgaaacgc cggaaatcat taccgatggt aacggctggc tggtaaaagg
     4981 cggtacagat ttgcatgcct tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga
     5041 tgatatcgcg acggtcgcgg gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg
     5101 cgatgtgatt gatgtagggc cactgcatat caccatcctt gaagccaatg attatcgtgt
     5161 tgatctggtt cgcattgtta aagagcaacc ggcgcacgat gaagatgagt aagcgttaac
     5221 gtaacgacat aatgggcgtg atatgtccat tatgccgggc gggcggcggt tggctgcccg
     5281 ccagccattt gggaaaatca cgtagcggca tcggctgtgc gtacaaatac ccttgcaaaa
     5341 tatgcacgcc atgacggcgc aaatacttcg cctgttcttg cgtttccacg cc
//
LOCUS       DAETPZ010000175         5296 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.177, whole genome shotgun sequence.
ACCESSION   DAETPZ010000175 DAETPZ010000000
VERSION     DAETPZ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.177"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..585
                     /locus_tag="K0W59_004003"
                     /pseudo
     CDS             1..585
                     /locus_tag="K0W59_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            649..1575
                     /gene="glsB"
                     /locus_tag="K0W59_004004"
     CDS             649..1575
                     /gene="glsB"
                     /locus_tag="K0W59_004004"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HBI3024800.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            1575..1934
                     /locus_tag="K0W59_004005"
     CDS             1575..1934
                     /locus_tag="K0W59_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HBI3024801.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQPPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            2073..3491
                     /locus_tag="K0W59_004006"
     CDS             2073..3491
                     /locus_tag="K0W59_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBI3024802.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            3718..5169
                     /gene="uxaB"
                     /locus_tag="K0W59_004007"
     CDS             3718..5169
                     /gene="uxaB"
                     /locus_tag="K0W59_004007"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBI3024803.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
BASE COUNT         1224 a         1234 c         1422 g         1416 t
ORIGIN      
        1 ttcccatcat tcttgcaggt aacggctact tacttaaaca tgcgccgaat gtgatgggct
       61 gtgctcagct cattgcccag gtgtttaaag atgcgggaat cccgcaaggc gtatatggct
      121 ggctgaatgc cgacaacgac ggtgtcagtc agatgattaa agactcgcgc attgctgctg
      181 tcacggtgac cggaagtgtt cgtgctggag cggcactgaa aaaatgcgta ctactatccg
      241 ccaacggttc tggcgaatgt taccccagaa atgaccgcgt ttcgggaaga aatgtttggc
      301 cccgttgctg caatcaccat tgcgaaagat gcagaacatg cgctggaact ggctaatgat
      361 agtgagttcg gcctttcagc gaccattttt accaccgacg aaacacaggc cagacagatg
      421 gcggcacgtc tggaatgcgg tggggtgttt atcaatggtt attgtgccag cgacgcgcga
      481 gtggcctttg gtggcgtgaa aaagagtggc tttggtcgtg agctttccca tttcggctta
      541 cacgaattct gtaatatcca gacagtgtgg aaagaccgga tctgacgcga catattcagc
      601 tctgatatac tcgcaggtct tttcagacct gcggtccagg agtagaaagt ggcagtcgcc
      661 atggataatg caattttaga aaacatcttg cggcaagtgc ggccgctcat tggtcagggt
      721 aaagtcgcgg attatattcc ggcgctggct acagtagacg gttcccgatt ggggattgct
      781 atctgtaccg ttgacggaca gctttttcag gccggagacg cgcaagaacg tttttccatt
      841 cagtctattt ccaaagtgct gagtctcgtt gtcgccatgc gtcattactc cgaagaggaa
      901 atctggcaac gcgtcggcaa agatccgtct ggatcaccgt tcaattcttt agtgcaactg
      961 gaaatggaac agggtattcc gcgtaatccg ttcattaatg ccggtgcgct ggtggtctgc
     1021 gatatgttgc aagggcgact aagcgcacca cggcaacgta tgctggaagt cgtgcgcggc
     1081 ttaagcggtg tgtctgatat ttcatacgat acggtggtag cgcgttccga atttgaacat
     1141 tccgcgcgaa atgcggctat cgcttggctg atgaagtcgt ttggcaattt ccatcatgac
     1201 gtgacaaccg ttctgcaaaa ctactttcat tactgcgctc tgaaaatgag ctgtgtagag
     1261 ctggcccgga cgtttgtctt tctggctaat caggggaaag ctattcatat tgatgaacct
     1321 gtggtgacgc caatgcaggc gcggcaaatt aacgcgctga tggcgaccag tggtatgtac
     1381 cagaacgcgg gggagtttgc ctggcgggtg ggactaccgg cgaaatctgg cgttggtggc
     1441 ggtattgtgg cgattgttcc gcatgaaatg gccatcgcag tctggagtcc ggaactggat
     1501 gatgcaggta actcgcttgc gggtatcgcc gtccttgaac aattgacgaa acagttaggg
     1561 cgttcggttt attaatgcag tctctcgatc cactcttcgc gcgtttatcc cgttcaaaat
     1621 ttcgctctcg ctttcgtctg ggcatgaaag agcgtcagta ttgcctggag aaaggcgcgc
     1681 cagtcatcga acaacatgcg gcggattttg tcgctaaacg tcttgctccg gcattaccgg
     1741 ctaatgacgg taagcaaccc cccatgcgcg ggcatccggt gtttatcgct cagcacgcca
     1801 ccgcgacctg ctgtcgcggt tgtctggcta agtggcataa cattccgcag ggtgtttcgt
     1861 taagcgaaga acaacaacgt tatatcgtgg cggtaatcta tcactggctg gttgtacaga
     1921 tgaatcagcc atgagaattg cggaaaacga taaaatagca ttcccagggt acctttattt
     1981 gccgccgctg ttagcgaaca tgctatcctt tatgagaatt tacttatcac gttgagataa
     2041 ttgccacccg caattgtatg gacagataat taatgcatgt acaccccatt tcaactttcc
     2101 gtttgttcca ggaaggtcat ctgctacgta atagcatcgc tatttttgcg ctaaccacgc
     2161 tgttctattt tattggtgca gagttacggc tggttcacga actttctctt ttctggccgc
     2221 tgaatggcgt aatggcgggg gtgtttgccc gctatgtctg gcttaatcga ctgcattact
     2281 atgcgatcag ttatgtggcg atgctggttt atgatgccat aaccaccgaa tggggactgg
     2341 tttcactggt tatcaatttc tccaatatga tgtttattgt taccgtcgcc ttactggtcg
     2401 tgcgggataa gcgtctgggg aaaaataagt atgagccagt aagtgcctta cggctattta
     2461 attactgtct gattgccgca ttattatgcg ctattgtcgg ggcgattggt tcggtcagta
     2521 ttgatagtct ggatttctgg cctttgcttg cggactggtt cagtgagcaa ttctcaacgg
     2581 gcgtgttgat cgtgccttgt atgctgacgt tggctattcc tggagtgctg ccgcgcttta
     2641 aagcagaaca gatgatgccc gccatcgcgc ttattgtgtc ggttattgcc tcggtagtca
     2701 ttggcggagc ggggagtctg gcgttcccgc tgcctgcatt aatctggtgt gcagtgcgct
     2761 atacgccgca ggtaacatgt ctgttgacct ttgtcaccgg tgcggtggaa atcgtactgg
     2821 tggcaaattc ggtgattgat atctcggtcg gttcgccgtt ctccattccg gaaatgttct
     2881 ccgcacgtct cggtattgcc acgatggcga tatgcccaat tatggtttct tttagcgtgg
     2941 cagcgatcaa tttgctaatg aagcaagttg cgctgcgagc cgactttgat tttctgactc
     3001 aggtttactc acggtccggt ctttatgagg cgctgaaaag tccatcgctg aaacagacac
     3061 aacatctgac ggtcatgctg cttgatatcg actatttcaa aagcattaac gataactatg
     3121 gacatgaatg tggcgataaa gtgttaagcg tgtttgcccg gcatattcag aagattgtcg
     3181 gtgataaggg gctggtggcg cgcatgggcg gcgaagaatt tgctgttgca gtgccgtcgg
     3241 tgaatcctgt cgatggtctg ctaatggcgg aaaaaatccg taaaggcgtt gaactgcaac
     3301 cattcacctg gcaacaaaaa acgctttatc tcacggtaag tattggcgtc ggtagtggtc
     3361 gcgcatcgta ccgaacgctg accgatgatt ttaataaatt gatggtcgaa gccgatacgt
     3421 gtctgtatcg ctcgaagaaa gatgggcgca accgtaccag cactatgcgt tacggtgagg
     3481 aagttgtctg acatctttat tcattccgcg cgatttgaat gcaatgtgtt ctaccacttt
     3541 atgatccagg aaaaggcgta tttcctcgat taaaccatga tccgcgccac acttttctca
     3601 taaaactgct cattaatcat caggagttga tcaaaacagg ggttcggcgt attaaagttt
     3661 gctaacctgt cgcaccaatt caaacaggtt gtatgactaa tcagaaggga acccattgtg
     3721 aaaacactaa atcgtcgcga ttttcccggt gcacagtatc cagaacgtat cattcagttt
     3781 ggtgaaggta acttcctgcg cgcctttgtt gactggcaaa tcgatctcct gaatgagcac
     3841 accgatctga attctggcgt ggtcgttgtt cgtccgattg aaacttcatt cccgccgtca
     3901 ctgagcacgc aggatggtct gtacaccacc attatccgtg gcctgaatga gaagggggaa
     3961 gcggtcagcg acgcgcgtct gattcgctcg gttaatcgtg aaatcagcgt ctacagtgaa
     4021 tacgatgaat tcctgaaact ggcgcacaac ccggaaatgc gctttgtttt ctctaacacc
     4081 accgaagcgg gtatcagcta ccatgcgggc gataaattcg atgacgcgcc agcggtaagc
     4141 tatccggcaa aactgacccg tctgctgttc gaacgcttta gccatttcaa cggtgcgctg
     4201 gataaaggtt ggatcatcat tccgtgtgag ttgattgact acaatggcga cgcgttgcgt
     4261 gaactggtgc tgcgctatgc gcaagagtgg gcactgccag aagcatttat tcaatggctg
     4321 gatcaggcta acagcttctg ttctacgctg gtggaccgta tcgttaccgg ttatccgcgc
     4381 gatgaagtgg ctaaactgga agaagaactg ggttatcacg atggtttcct cgacaccgct
     4441 gaacactttt acctgtttgt gattcagggg ccgaaatcct tagcgactga gctgcgcctg
     4501 gacaaatatc cgctcaacgt gctgattgtt gacgatatta aaccgtataa agaacgcaaa
     4561 gtggcaatcc tcaacggtgc gcacaccgcg ctggtgccgg tggcgttcca ggcagggctg
     4621 gataccgtag gtgaagcgat gaacgatgct gaaatttgcg cattcgttga aaaagccatc
     4681 tacgaagaaa ttattccggt actggatttg cctcgtgatg aactggaatc tttcgccagt
     4741 gcagtcaccg ggcgtttccg taacccgtac attaagcatc agctgctgtc tatcgcgctc
     4801 aacggtatga ccaagttccg cacccgcatc ctgccgcagc tgctggcagg gcagaaggca
     4861 aacggcacac ttccggcgcg ccttactttc gcattagcgg cattgattgc gttctatcgt
     4921 ggtgagcgta acggagaaac atatccggta caggacgatg cacactggct ggaacgttat
     4981 cagcaactct ggagccagca tcgtgaccgt gttatcggta cccaggaact ggtagcgatt
     5041 gtgttggcag aaaaagacca ctgggagcag gacctgacgc aagtgcctgg tctggtcgag
     5101 caggttgcta atgacctgga tgcgattctt gaaaaaggga tgcgcgaagc tgtacgtccg
     5161 ttgtgctaat aacaccgtta cttctgaacc cggtttcgac cgggtttctt atttcactat
     5221 attagttaca acatcctata tgcctttatc attaacaggt gtcacatgca gcgcctgtta
     5281 atgttgtaag cttgac
//
LOCUS       DAETPZ010000176         5265 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.178, whole genome shotgun sequence.
ACCESSION   DAETPZ010000176 DAETPZ010000000
VERSION     DAETPZ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.178"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..166
                     /locus_tag="K0W59_004008"
     CDS             <1..166
                     /locus_tag="K0W59_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HBI3024804.1"
                     /translation="THYGGAAGPVIFGVGYNDIKSWWLVGAVLTILTFLVHITLGVWW
                     WNMLIGWNML"
     gene            349..2529
                     /locus_tag="K0W59_004009"
     CDS             349..2529
                     /locus_tag="K0W59_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HBI3024805.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNHQM"
     gene            complement(2670..3953)
                     /locus_tag="K0W59_004010"
     CDS             complement(2670..3953)
                     /locus_tag="K0W59_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HBI3024806.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(4088..5158)
                     /locus_tag="K0W59_004011"
     CDS             complement(4088..5158)
                     /locus_tag="K0W59_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBI3024807.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKIFASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(5136..>5265)
                     /locus_tag="K0W59_004012"
     CDS             complement(5136..>5265)
                     /locus_tag="K0W59_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBI3024808.1"
                     /translation="FPPPVKDGREYLFHESAVKVDLNRPVTGSLLKRIRNGKKAKS"
BASE COUNT         1253 a         1290 c         1331 g         1391 t
ORIGIN      
        1 tactcactat ggcggcgcgg caggtccggt catctttggc gtgggttaca acgatattaa
       61 atcctggtgg ttggtcggtg cggtactgac gatattaacc ttcttggtgc atatcaccct
      121 cggcgtgtgg tggtggaata tgctgatcgg ctggaacatg ctgtaaatat actcgtcata
      181 cttcaagttg catgtgctgc gtctgcgttc gctcacccca gtcacttact tatgtaagct
      241 cctggggatt cactcacttg tcgccttcct gcaactcgaa ttatttagag tatatccatt
      301 tattatcttt ctgcgcactt cacggtgcgc agatatctgg agcatttgat gatcaagtta
      361 tctgaaaaag gcgtgtttct cgccagtaat aacgaaataa ttgccgaaga acatttcacc
      421 ggcgaaatta aaaaagaaga agcaaaaaaa ggcactattg cctggtctat tctctcttct
      481 cataatacgt ccggaaatat ggataaactt aaaattaagt ttgattcatt agcctctcac
      541 gatattacct ttgttggtat tgtacagacc gctaaagcgt ccggaatgga acgtttcccg
      601 ctgccgtatg tgctgaccaa ctgccataac tcactctgcg ccgtcggcgg caccattaac
      661 ggtgatgacc atgtttttgg tttatcggca gctcagcgtt atggcggtat ttttgtgcct
      721 ccgcatattg cggtcatcca tcaatatatg cgtgagatga tggctggcgg gggcaaaatg
      781 atcctcgggt cagacagcca cacccgttac ggtgcattag ggacaatggc agtcggtgag
      841 ggtggcggtg agttggtaaa acagctgctt aatgacacct gggatatcga ctatccgggc
      901 gtggttgcgg tgcatctgac cggaaaacct gcgccgtatg tggggccaca ggatgtggcg
      961 ctggctatca ttggcgcggt gttcaaaaac ggttacgtca aaaacaaagt catggagttc
     1021 gttggaccgg gcgttagcgc gctctctacc gatttccgta acagcgttga cgtgatgacc
     1081 acggaaacca cctgtttaag ctccgtctgg caaactgatg aagaagtgca taactggctg
     1141 gcgctgcacg gtcgcggcca ggattactgc cagcttaacc ctcaaccgat ggcgtactac
     1201 gatggctgca tcagcgttga tttaagcgcc atcaaaccaa tgattgcgct gccgttccac
     1261 ccgagcaacg tgtatgaaat cgacacactg aaccagaacc tgaccgacat tctgcgtgag
     1321 attgaaattg agtccgaacg cgtggcgcac ggtaaagcca aactctcgct gctggataaa
     1381 gtggaaaatg gtcgcctgaa agtgcagcag gggattatcg cgggctgttc tggcggtaac
     1441 tacgaaaacg tcatcgcggc ggcgaatgca ctgcgcggtc aatcctgtgg caatgacacc
     1501 ttctcgctgg cggtttaccc gtcatcacag ccggtgttta tggatctagc caaaaaaggt
     1561 gtggtagcag atttgattgg cgcaggcgca atcatcagaa ccgcattctg tggcccatgc
     1621 tttggcgcgg gcgatacgcc aatcaacaac ggtttgagta ttcgccacac cacgcgtaac
     1681 ttcccgaacc gcgaaggctc taagccagcc aatgggcaga tgtcagcggt ggcgctgatg
     1741 gacgctcgtt ctatcgctgc gactgcggca aacggtggct atttaacctc tgccagcgaa
     1801 cttgattgct gggacaacgt gccggagtac gccttcgatg taacgccgta taaaaaccgt
     1861 gtttatcagg gctttgtgaa aggggcaact cagcaaccgc tgatttacgg accgaacatt
     1921 aaagactggc cggaattggg tgcgctgact gacaatatcg tcctgaaagt gtgctcgaag
     1981 atcctcgacg aagtcaccac caccgacgaa ctgattcctt ccggtgaaac ctcttcttat
     2041 cgttcaaatc cgattggtct ggcggggaat gtcagcgagc tgacagaggt gtttgcgcgc
     2101 attaagcaga ttgctggtca ggagcatatt gatccgctgc aaactgaaat tggcagcatg
     2161 gtctatgcgg tgaaaccagg cgatggttct gcgcgtgaac aggcggcgag ctgccagcgt
     2221 gtgattggcg gtctggcgaa tattgccgag gagtacgcga ctaaacgcta ccgttctaac
     2281 gtcatcaact gggggatgtt accgctgcag atggcggaag tgccaacctt tgaagtgggg
     2341 gattacattt acatccctgg cattaaagcg gcgctggata atccgggtac gacgtttaaa
     2401 ggttatgtga tccatgaaga tgcgccggta acggaaatta cgctttacat ggaaagcctg
     2461 acggctgaag agcgcgagat tatcaaggcg ggtagtttga ttaacttcaa taaaaaccat
     2521 cagatgtaaa aagcgccatg tgaatgtagg tcgcattcgg cacttattgt cggatgcgat
     2581 gctggcgcat cttatccgac ctacaaatcg catcgaaccg taggccggat aaggcgttta
     2641 cgccgcatcc ggcaaatagt taatagctct tacttcttcg cctctgcaac cactttgctg
     2701 cccacgccgc ggttattgta ttcccacatg cggttgtagt tagtgtcatt cagattgcgc
     2761 tgtatttcgt cgttatcatc tacgctgccg gtattacccg caaacggacg attagagatc
     2821 accgcatcgg cccacggttt agccgtgtta aaaccttcgt tgatggcgct atcacggatc
     2881 accacctgac cgttggtatt agcatcaaca tccagcgagc ggcccagttg cgccacgcca
     2941 tcaccggaag cattgaaacg gctgtttacg gcgaggaaac cgtaatagat gttagacagc
     3001 gtagccggtg caaacacata tgcttcttgc tgggtacgtg agttcaccac gcggaattcg
     3061 gtgttatcga acaccactgc gccgcgacca gaaacgatat ccacatcccc ttcaatatag
     3121 ctgttggtca ccagcgtacg cggctgacgg ttggtttcca gacggttctg cacaccgctg
     3181 ttggtgacaa agaaggtgtt ctgacgaccg agaatgttaa cgttgttaat ctgtacctgg
     3241 tcaccgtcag tacgcagtgc taccgccgga tggttacctg catctacact atcgcccagc
     3301 gtgttttcga tggtcagatt ttgcagttgc agtccattgt tttgtgacca gaagaccgca
     3361 gaacacagaa caccgatact gtcgctgcgt ttgctctggc agctatcgta catataccac
     3421 gctggtttac ctggcatata tttgccgcgc gggttgacgt cgtgacgcca gtcggcaggg
     3481 ctcatgcccc catcaaggga aagcccaatc ttcacatcaa tcggtttttc acctgtaccg
     3541 tacagagtga ttccacctgg agcagcaggg acatacaccg ttccctgata ctcaccaggc
     3601 atcacggcaa tatactggcg cttgttggtg cgcttgataa ttgccgcatc taccgccgcc
     3661 tgaatcgtgg tatgcgttac accttgagta cccgccgggc cgacaacaaa gtcaggttgc
     3721 gcaggcaggg ttatcgggga cggattccac gctgcagcac ctggtgtcag ggatgcaaaa
     3781 tagtgttgcg catcgaaatt cagcgcttct tttgccgaca gaatcgggcg agaagaggta
     3841 ccaggcgcag tttgatcaga aggacgttga tcgggtggtg ttgagctaca ggcggtcagc
     3901 gtcacgccaa aagccaatgc cagcgccaga cgggaaactg aaaatgtgtt cacaggttgc
     3961 tccgggctat gaaatagaaa aatgaatccg ttgaagcctg cttttttata ctaagttggc
     4021 attataaaaa agcattgctt atcaatttgt tgcaacgaac aggtcattat cagtcaaaat
     4081 aaaatcatta tttgatttca attttgtccc actccctgcc tctgtcatca cgatactgtg
     4141 atgccatggt gtccgacttg tgcccgagaa gatgttgagc aaacttatcg cttatctgct
     4201 tctcatagag tcttgcagac aaactgcgca actcgtgaaa ggtaggtgga tccccttcga
     4261 aggaaagacc tgatgctttt cgtgcgcgca taaaatacct tgatactgtg ccggatgaaa
     4321 gcggttcacg acgagtagat gcaattatgg tttctccgcc aagaatctct ttgcatttat
     4381 caagtgtttc cttcattgat atcccgagag catcaacatg caatgttgta gggatggcaa
     4441 tttttacgcc tgttttgctt tgctcgacat aaagatatcc atctacgata tcagaccact
     4501 tcatttcgca taaatcacca actcgttgcc cggtaacaac agccagttcc attgcaagtc
     4561 tgagccaaca tggtgatgat tctgctgctt gataaatttt caggtattcg tcagccgtaa
     4621 gtcttgatct ccttacctct gattttgctg cgcgagtggc agcgaccggg tttgttgtta
     4681 tatggccttc agctattgcc tctcggaatg catcgctcag tgttgatctg attaacttgg
     4741 ctgatgccgc cttgccctcg tctatgtatc cattgagcat tgccgcaatt tcttttgtgg
     4801 tgatgtcttc aagtggagca tcaggcagcc ccctccttat tgctttaatt ttgctcatgt
     4861 aatttatgag tgtcttctgc ttgattcctc tgctggcgaa gattttttcg tagcgatcaa
     4921 gccatgaatg taacgtaaca gaattatcac tgttgattct cgctgtcaga ggcttgtgtt
     4981 tgtgtcctga aaataactca atgttggcct gtatagcttc agtgattgcg attcgcctgt
     5041 ctcggcctaa tccaaactct ttacccgtcc ttgggtccct gtagcagtaa tatccattgt
     5101 ttcttatata aaggttaggg ggtaaatccc ggcgctcatg acttcgcctt cttcccattt
     5161 ctgatcctct tcaaaaggct acctgttact ggtcgattta agtcaacctt taccgctgat
     5221 tcgtggaaca aatactctct tccatcctta accggaggag ggaat
//
LOCUS       DAETPZ010000177         5260 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.179, whole genome shotgun sequence.
ACCESSION   DAETPZ010000177 DAETPZ010000000
VERSION     DAETPZ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.179"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            217..474
                     /locus_tag="K0W59_004013"
     CDS             217..474
                     /locus_tag="K0W59_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBI3024809.1"
                     /translation="MTLPVFITVIADHDKPQPSGCLLESQGSLCPICRQRITHETGWN
                     VHHKVKKVMGGGEELSNLVLLHPNCHRQLHSCEAGSLKKGL"
     gene            617..838
                     /locus_tag="K0W59_004014"
                     /pseudo
     CDS             617..838
                     /locus_tag="K0W59_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(859..921)
                     /locus_tag="K0W59_004015"
                     /pseudo
     CDS             complement(859..921)
                     /locus_tag="K0W59_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1371..2783
                     /gene="ospC1"
                     /locus_tag="K0W59_004016"
     CDS             1371..2783
                     /gene="ospC1"
                     /locus_tag="K0W59_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HBI3024810.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHGSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2887..3036)
                     /locus_tag="K0W59_004017"
                     /pseudo
     CDS             complement(2887..3036)
                     /locus_tag="K0W59_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            3112..4809
                     /gene="ospD3"
                     /locus_tag="K0W59_004018"
     CDS             3112..4809
                     /gene="ospD3"
                     /locus_tag="K0W59_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HBI3024811.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLNPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGFSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
     gene            4926..5045
                     /locus_tag="K0W59_004019"
                     /pseudo
     CDS             4926..5045
                     /locus_tag="K0W59_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
BASE COUNT         1779 a          986 c         1017 g         1478 t
ORIGIN      
        1 cgactctttt atcaacgctt acgtaattaa gggacacagc ctagtctcca ggattcccgg
       61 ggcggttcaa tcaaagcgca gaacttctgc gtcttcttcg tcaaaaaaat catcttcgtg
      121 aagcttcacg acatagcggg gaagtttgct tctttgagag gcgggtttac gtttacgggg
      181 tttagctgaa cgggccatat aaccacctga aagacaatga cattgcctgt ttttataacg
      241 gtaattgcag accatgacaa accgcagccg tcaggctgcc tactcgagtc acaaggctct
      301 ttgtgtccga tatgccggca gcgcattacc catgaaactg gctggaacgt acaccataag
      361 gtgaagaaag ttatgggggg cggtgaagaa ttatctaacc ttgtcttgtt acatccaaat
      421 tgccatcgac agttacacag ttgtgaagcc ggctctctca aaaagggctt ataaaggctt
      481 gagcggtgta ccgggaaacc ggtatgcacc gttcttaggg gagggcagtc cagcaatgaa
      541 ctgtccttac ccgacaaaca tccaaaacga acggaacgtg ctggaatccg catataaccc
      601 gttgtaaagt gaggaaatgg acacctcact tgctcatgag aacgcccgcc tgcgggcact
      661 gttgcagacg caacaggaca ctatccacca gatggctgaa tacaaccgcc tgctctcaca
      721 gcggatggcg gcttatgctt ccgaaatcaa ccgactgaag gcgctggttg cgaaactgca
      781 gcgtatgcag ttcggtaaaa gctcagaaaa acttcgcgca aaaaccgaac ggcagattcc
      841 attttcacgg gcgatctacg ccacacaggc tctgggttgc agtgactgct ccattaaggc
      901 gattttgaac tcataaggga agtaacaaaa aaactgcaaa agtacaataa ttatatatgg
      961 ccaaactaac ctctattaaa aaacatcagt ttttcacact tagttgagct tcatgaaaaa
     1021 atcatcgagg atttcattgc agaatacaca ttccactaac tggaagcact ccataagagg
     1081 aatcagagaa agtatcgttt ttttatagta aaattcattg ctgttcaata aaattgatat
     1141 agttattaaa ttatgaaaat aatttcacaa aaaagattaa cttgagttct taagcatcgg
     1201 attttagata ttttgaaata tctggcatca cataacatca aaagtatata atacccttat
     1261 ctaattacat gcagtatatt gtatatatac tcaaaaattg taattcatac cctgctatat
     1321 attgtaggga aagatattaa ttaaaactgt tttcatataa ggttcatttt atgaatatat
     1381 cagaaacact gaactcagca aatacccaat gcaatataga ttctatggat aacagattac
     1441 atacattgtt tccaaaagtg acatcagtgc gaaacgctgc acaacaaact atgccagatg
     1501 aaaaaaattt aaaagatagt gcaaatatta ttaaagattt ctttaggaaa actatagcag
     1561 cacagagtta tagtagaatg ttctctcaag gctctaactt taaatcttta aatatagcaa
     1621 ttgatgcacc atcagacgct aaagcctcat ttaaggctat tgagcacctt gacagattat
     1681 cgaagcatta tatatctgaa ataagggaaa aacttcatcc tctttctgca gaggaactca
     1741 atttgctttc gctaattatt aattctgatt taatcttcag acatcaaagt aattctgatt
     1801 tgtctgataa aattttaaac attaagtcat tcaataaaat tcagtctgaa ggaatatgca
     1861 caaaacgaaa cacatacgct gatgatataa aaaaaatagc taatcatgac tttgtgtttt
     1921 ttggcgttga aatctctaac catcagaaaa aacaccccct gaatacaaaa catcacactg
     1981 ttgattttgg tgcaaatgcg tatatcattg atcatggctc tccatatgga tatatgacat
     2041 taaccgatca ctttgataat gctattccac ctgtttttta ccatgagcac caatcatttt
     2101 tagataaatt ttcagaggtt aataaagaag ttagtcgata cgtacatgga agtaaaggaa
     2161 ttatagatgt accaatattc aatactaaag atatgaagtt agggctcgga ttatacctga
     2221 ttgactttat tagaaaaagt gaagaccaaa gcttcaagga gttttgctat ggaaaaaatc
     2281 ttgcccctgt ggatctggat agaatcataa actttgtttt tcagccagag taccatatac
     2341 ctaggatggt aagtacagaa aacttcaaaa aagttaagat tagagaaata tctttagagg
     2401 aggctgttac agcatctaat tacgaagaaa ttaacaagca ggtcactaac aaaaaaattg
     2461 ctctccaggc tctttttctt tcgattacta atcaaaaaga ggatgtcgcc ttatatatat
     2521 tatctaattt tgagataact agacaagatg ttatttccat aaagcatgag ttgtatgata
     2581 ttgagtatct acttagcgct cataattcaa gctgtaaagt acttgagtat tttatcaata
     2641 agggattggt tgatgtaaac acaaagttca aaaaaactaa tagtggggat tgtatgttgg
     2701 ataacgcaat aaaatatgag aatgcagaaa tgataaaact attattgaaa tatggtgcaa
     2761 catctgacaa taaatatatt taatcaaaat tgaatatcgt ttagcaaaag gcagtatttg
     2821 cacaaaatat cgatatattg tttattgtca gtatggctca atgtgataat agttggaaag
     2881 tttgatgggt ttcgccccgt tgtagcggta gtcgaactgg aaggtcttca ggcactgctt
     2941 acagcgatag agcagccccc ctgaactgga atggccgttc cggatacccc cctgagtttc
     3001 agagtaacgg ggacaaacca catcaatctt tgccatcaat catccaaagg gcaaagagta
     3061 caacaacact aagtctgcgt cacaacccat caatgaaagg aatatataca tatgccatca
     3121 gtaaatttaa tcccatcaag gaaaatatgt ttgcaaaata tgataaataa agacaacgtc
     3181 tctgttgaga caatccagtc tctattgcac tcaaaacaat tgccatattt ttctgacaag
     3241 aggagttttt tattaaatct aaattgccaa gttaccgatc actctggaag acttattgtc
     3301 tgtcgacatt tagcttccta ctggatagca cagtttaaca aaagtagtgg tcacgtggat
     3361 tatcatcact ttgcttttcc ggatgaaatt aaaaattatg tttcagtgag tgaagaagaa
     3421 aaggctatta atgtgcctgc tattatttat tttgttgaaa acggttcatg gggagatatt
     3481 attttttata ttttcaatga aatgattttt cattccgaaa aaagcagagc actagaaata
     3541 agtacatcaa atcacaatat ggcattaggc ttgaagatta aagaaactaa aaatgggggg
     3601 gattttgtca ttcagcttta tgatcccaac catacagcaa ctcatttacg agcagagttt
     3661 aacaaattta acttagctaa aataaaaaaa ctgactgtag ataattttct tgatgaaaaa
     3721 catcagaaat gttatggtct tatatccgac ggtatgtcta tatttgtgga cagacatact
     3781 ccaacaagca tgtcctccat aatcagatgg cctaataatt tacttaaccc caaagttatt
     3841 tatcacgcga tgcgtatggg attgactgag ctaatccaaa aagtaacaag agtcgtacaa
     3901 ctatctgacc tttcagacaa tacgttagaa ttacttttgg cagccaaaaa tgacgatggt
     3961 ttttcaggat tgcttttagc tttacaaaat gggcattcag atacaatctt agcatacgga
     4021 gaactcttgg aaacttctgg acttaacctt gataaaacgg tagaactact aactgcggaa
     4081 ggaatgggag gacgaatatc gggtttatcc caagcacttc aaaatgggca tgcagaaact
     4141 atcaaaacat acggaaggct tctcaagaag agagcaataa atatcgaata caataagctg
     4201 aaaaatttgc tgaccgctta ttattatgat gaagtacaca gacagatacc cggactaatg
     4261 tttgctcttc aaaatggaca tgcagatgct atacgcgcat acggtgagct cattcttagc
     4321 ccccctctcc tcaactcaga ggatattgta aatttgctgg cctcaaggag atatgacaat
     4381 gttcccggac ttctgttagc attgaataat ggacaggctg atgcaatctt agcttatggt
     4441 gatatcttga atgaggcaaa acttaacttg gataaaaaag cagagctgtt agaagcgaaa
     4501 gattctaatg gtttatctgg attgtttgta gccttgcata atggatgtgt agaaacaatt
     4561 attgcttatg ggaaaatact tcacactgca gaccttactc cacatcaggc atcaaaatta
     4621 ctggcagcag aaggcccaaa tggggtatct ggattaatta tagcttttca aaataggaat
     4681 tttgaggcaa taaaaactta tatgggaata ataaaaaatg aaaatattac acctgaagaa
     4741 atagcagaac acttggacaa aaaaaatgga agtgattttc tagaaattat gaagaatata
     4801 aaaagctgaa tattatctcg tggtgataca ctgaatttga ggtaaccggc tcatttaaac
     4861 cgtctggtct gttacctccg gttccacaaa aataatgtcc atcattttga agtggacact
     4921 atcgtatgga ataccggacc tggatcactg aagctttacg ccttcatttc gaagaacatt
     4981 taccacgggt tgtggccgga cgtcgcctgg gcgtaccaaa atcaactgtc tgtggtatgt
     5041 tcgtgtgaac cgcccccggt atcccgctca tgctggcgaa cccagacacg cagtgaaccg
     5101 ccccgggttt cctggagagt gttttatctg tgaaccacgg cagcctggcg gctgcaaatc
     5161 ggggtgtggc agaatatgag ctttcagagt aaccatccag aatgagttcc catgtcacac
     5221 aacctggagc accagaaagt ccacaccaga atggtgaaag
//
LOCUS       DAETPZ010000178         5238 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.180, whole genome shotgun sequence.
ACCESSION   DAETPZ010000178 DAETPZ010000000
VERSION     DAETPZ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.180"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..374)
                     /locus_tag="K0W59_004020"
     CDS             complement(<1..374)
                     /locus_tag="K0W59_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HBI3024812.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILN"
     gene            509..604
                     /locus_tag="K0W59_004021"
     CDS             509..604
                     /locus_tag="K0W59_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBI3024813.1"
                     /translation="MLVFQMRYQMRYVDKTSTVLKQTKKSDYADK"
     gene            730..1467
                     /gene="erm(B)"
                     /locus_tag="K0W59_004022"
     CDS             730..1467
                     /gene="erm(B)"
                     /locus_tag="K0W59_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBI3024814.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1412..1582
                     /locus_tag="K0W59_004023"
     CDS             1412..1582
                     /locus_tag="K0W59_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HBI3024815.1"
                     /translation="MSKYCLFLIVIYYLTGGNNSMSRFCKFGKLHVTKGNVDKLLGIL
                     LTASKELKRSLD"
     gene            2097..2546
                     /locus_tag="K0W59_004024"
     CDS             2097..2546
                     /locus_tag="K0W59_004024"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024816.1"
                     /translation="MTTILSDYNKSILIELLNTNRQIIVVHGDDIDDYYTDSCYDIIF
                     IDYYDDDDFTYNGQEIWRGDSIYLVKSYQDEIQNRIEKGQELIFVNKENDISFSESIL
                     TYYHLFDYAGKTFEIDGQIIDEQTKVFAAPERRCACRTCWKVPARKT"
     gene            2507..2848
                     /locus_tag="K0W59_004025"
     CDS             2507..2848
                     /locus_tag="K0W59_004025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024817.1"
                     /translation="MSHVLEGASQEDLNLYRAEVERDQAYGNWRDFFLNKKGSVTSVS
                     GDANLDQIADVSYLLDTFFAKVTRTALQNAASIAGLMITTEAMVAEAPKKDEPAMPPG
                     GGMGGMGGMDF"
     gene            3075..4607
                     /locus_tag="K0W59_004026"
     CDS             3075..4607
                     /locus_tag="K0W59_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBI3024818.1"
                     /translation="MPRLATSRRQAAGCAPLPSAHTGSRYARHAPERTLLYALVEAHY
                     PDFIARIEAEGRSLPGYVREAFDAYLRCGVLEHGFLRVVCEHCRAERLVAFSCKKRGF
                     CPSCGARRMAESARHLVEEVFGPRPVRQWVLSFPYPLRFLFASKPEAIGPVLGIVQRV
                     IAGWLADQAGIDRASAQCGAVTLIQRFGSALNLNIHFHMLWLDGVYVEATELPRRELR
                     LHRARAPTTAQLTQLAAAIAHRVCRHLTRKGWLEGEGESAFLADSAAGDDSMDGLRMS
                     SITYRIATGRDAGCKVVTLQTLPGDAGSLEGEAGKVGGFSLHAGVAAEAHESHKLEKL
                     CRYITRPAISEKRLSIALQGRVRYQLKTPWRNGTTHVEWDPVDFIAKLAALVPPPRAH
                     LTRFHGVFAPNAALRAQLTPSGRGRRHDAAVEPADASANDAPRSPEEKRRSMSWAQRL
                     KRVFSIDVTACVHCGGTVRIVASIEEPAAIRAILGHFVKQGAREEAHYRPAARAPPVQ
                     AA"
     gene            complement(4930..>5238)
                     /locus_tag="K0W59_004027"
     CDS             complement(4930..>5238)
                     /locus_tag="K0W59_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HBI3024819.1"
                     /translation="RFINTDKAPAYGRALALLKREGRCPSDVEHRQIKYRNNVIECDH
                     GKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPLGEMRLVSRVFEM
                     "
BASE COUNT         1355 a         1353 c         1343 g         1187 t
ORIGIN      
        1 ttgttgagga ttttacccag aaaccggtat gcagctttgc tgttacgacg ggaggagaga
       61 taaaaatcga cagtgcggcc ccggctgtcg acggcccggt acagatacgc ccagcggcca
      121 ttgaccttca cgtaggtttc atccatgtgc cacgggcaaa gatcggaagg gttacgccag
      181 taccagcgca gccgtttttc catttcaggc gcataacgct gaacccagcg gtaaatcgtg
      241 gagtgatcga cattcactcc gcgttcagcc agcatctcct gcagctcacg gtaactgatg
      301 ccgtatttgc agtaccagcg tacggcccac agaatgatgt cacgctgaaa atgccggcct
      361 ttgaatgggt tcatgtgcag ctccatcagc aaaaggggat gataagttta tcaccaccga
      421 ctatttgcaa cagtgccaaa ttttgtataa taggaattga agttaaatta gatgctaaaa
      481 atttgtaatt aagaaggagg gattcgtcat gttggtattc caaatgcgtt atcaaatgcg
      541 ttatgtagat aaaacatcta ctgttttgaa acagactaaa aaaagtgatt acgcagataa
      601 ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta aacagataac
      661 taaaattaca aacaaatcgt ttaacttctg tatttgttta tagatgtaat cacttcagga
      721 gtaaattaca tgaacaaaaa tataaaatat tctcaaaact ttttaacgag tgaaaaagta
      781 ctcaaccaaa taataaaaca attgaattta aaagaaaccg ataccgttta cgaaattgga
      841 acaggtaaag ggcatttaac gacgaaactg gctaaaataa gtaaacaggt aacgtctatt
      901 gaattagaca gtcatctatt caacttatcg tcagaaaaat taaaactgaa tactcgtgtc
      961 actttaattc accaagatat tctacagttt caattcccta acaaacagag gtataaaatt
     1021 gttgggaata ttccttacca tttaagcaca caaattatta aaaaagtggt ttttgaaagc
     1081 catgcgtctg acatctatct gattgttgaa gaaggattct acaagcgtac cttggatatt
     1141 caccgaacac tagggttgct cttgcacact caagtctcga ttcagcaatt gcttaagctg
     1201 ccagcggaat gctttcatcc taaaccaaga gtaaacagtg tcttaataaa acttacccgc
     1261 cataccacag atgttccaga taaatattgg aagctatata cgtactttgt ttcaaaatgg
     1321 gtcaatcgag aatatcgtca actgtttact aaaaatcagt ttcatcaagc aatgaaacac
     1381 gccaaagtaa acaatttaag taccgttact tatgagcaag tattgtctat ttttaatagt
     1441 tatctattat ttaacgggag gaaataattc tatgagtcgc ttttgtaaat ttggaaagtt
     1501 acacgttact aaagggaatg tagataaatt attaggtata ctactgacag cttccaagga
     1561 gctaaagagg tccctagact agcaagaagt acacaagaag ccttaaagat tatagaaaag
     1621 cttttccatg aaatgcttaa cattatttaa tgttaacatg tggctttgtg gtaactacca
     1681 cgagtggtaa cttgtaacat gaaaaaagtt acaagttaca agtggtttat ttacgaacat
     1741 aaaggctagg aaagcacttg ataagtatat caagtgcttt ttttccgttc gattttacta
     1801 tccaagatac cgcttgacgt acctaatatt ttagtgtgct aagggctttg ctttttctgg
     1861 ttatagttcg ttcaaaaaag accgcaaggg taaatgcacg atcatagatc gcccttgcag
     1921 ctttttaatc tctccacttt tttataacca agcaaaccac caagcacact gaaaaaataa
     1981 ttaggaacta aagcgaagtg cgtatcttgg ataaatcttc actcgatttt aaaagattaa
     2041 gcacccagcc aaagcgccta aaaaaataat ttttttaaag aaaggaagta atttaaatga
     2101 ctacaatttt aagcgactac aataaatcta tcctaattga attactaaac accaatcgcc
     2161 aaattatcgt cgttcatggc gacgatattg atgattatta cacagattct tgctatgaca
     2221 ttatctttat tgattactat gatgatgatg atttcactta taacggacag gaaatatggc
     2281 gtggcgatag catttatctt gtaaaaagtt atcaagatga aattcaaaac cgtatagaaa
     2341 aaggacaaga gctgatattt gttaataaag aaaatgatat atcattttca gaatctattt
     2401 taacttatta ccatctattt gattatgctg gtaaaacgtt tgaaatagac ggtcaaatca
     2461 ttgatgaaca aacaaaagtt tttgccgcac ccgagcgccg ctgcgcatgt cgcacgtgct
     2521 ggaaggtgcc agccaggaag acctgaacct ttaccgcgcg gaagtggagc gcgaccaggc
     2581 ctatggcaac tggcgcgatt tcttcctgaa caagaagggc agcgtcacat ccgtgagcgg
     2641 tgatgccaac ctcgaccaga tcgcggacgt gtcgtacctg ctggatacct tctttgccaa
     2701 ggtcacccgc accgcgttgc agaacgccgc ctccatcgcg ggcctgatga tcaccaccga
     2761 agccatggtg gccgaggccc cgaagaagga cgagccggcg atgccgccgg gcggcggcat
     2821 gggtggcatg ggcggcatgg atttctgatg cggttggccc ggtcgtcagg gaaccggacc
     2881 gcgccagcgc ggtccgatcc cggcaacgac ccgacatcaa ggccccaagg acggggccgg
     2941 agcccggcag cgatgccggg ctttttgttg tgcccgcgcc gcggcaatgt ctgacgcgaa
     3001 gatcagaacg caccgatacg aacgtgcgaa cacaggtgca accctgagca gccgtccccg
     3061 caccggagcg ctgcgtgccg cgcctcgcca catcccggcg gcaagccgcg ggatgcgcgc
     3121 cactgccgtc cgcccacacc ggttcgcggt acgcgcgcca cgcgcccgag cgcacgctgc
     3181 tgtacgcgtt ggtagaggcg cactacccgg acttcattgc acggatcgaa gcggagggcc
     3241 gctcgctgcc cgggtatgtc cgcgaggcgt tcgatgccta cctgcgttgc ggcgtgctcg
     3301 agcacggctt cctgcgggtg gtgtgcgagc actgccgtgc agagaggctg gtggccttct
     3361 cctgcaagaa gcgcgggttc tgcccgagtt gcggcgcgcg acgcatggcc gagagtgcgc
     3421 ggcacctggt cgaggaggtg ttcggcccgc ggcctgtgcg gcaatgggtg ctgagctttc
     3481 cgtacccctt gcgtttcctg ttcgccagca agccagaagc cattggcccg gtgctgggca
     3541 tcgtgcagcg cgtgatcgcc ggctggttgg ccgatcaagc cggcatcgac cgcgccagcg
     3601 cccagtgcgg cgcggtgacg ctgatccagc gtttcggcag cgcgctgaac ctgaacatcc
     3661 acttccacat gctgtggctc gacggcgtgt acgtggaagc caccgagctg ccgcggcgcg
     3721 aactgcgcct gcaccgcgcc cgtgcgccca ccaccgcgca gttgacccag ctggcagctg
     3781 ccatcgcgca ccgggtgtgt cggcacctga cgcgcaaagg ctggctcgaa ggggagggcg
     3841 aatcggcctt cctggcagac agcgctgcag gcgacgacag catggatggg ctgcggatga
     3901 gttcgatcac ctaccgcatc gccaccggcc gcgacgctgg ctgcaaggtc gtcacgctgc
     3961 aaacgctgcc cggtgacgcc ggttcgctgg agggcgaagc cggcaaggtc ggcggcttct
     4021 cactgcatgc cggcgtggcg gccgaagcac acgaaagcca caagctggaa aagctgtgcc
     4081 gctacatcac gcgcccggcg atcagcgaga agcggctgtc gatagcgctc cagggcaggg
     4141 tgcgttacca gctcaagacc ccgtggcgca atggcaccac gcatgtggaa tgggatccgg
     4201 tggatttcat cgccaagctg gcggcgctgg tcccgccacc gcgcgcccac ctgacccgct
     4261 tccacggggt gtttgccccg aacgccgccc tgcgcgcaca gctgacgcca tcggggcgcg
     4321 gcaggcggca tgacgccgct gtggagccgg cggacgcaag cgcgaacgac gcgccgcgca
     4381 gccccgagga gaagcgccgt tcgatgagct gggcgcaacg cctcaagcgg gtcttttcca
     4441 tcgacgtcac cgcctgcgtc cactgcggtg gcaccgtgcg gatcgtcgcc agcatcgagg
     4501 aacctgccgc catccgcgcc atccttggcc acttcgtgaa gcagggcgcg cgggaagaag
     4561 cgcactacag gcccgcagcg cgcgcaccgc cagtgcaagc cgcgtgacga tctgccggct
     4621 gcacagccga cggcgaaacc ggaatccgag ccgatgcggc cacgatccgc agggcggcgc
     4681 tcggcccgct gtcgggaatc agcgaagcat ggctgctgac aacgccgctg cgtggccccg
     4741 cgatgccgaa atcccactca cagacgtccg atccgtgccc aaaacggggc ttgcgcgacc
     4801 gccgcctacc cagcagactg cccgaaaagg gcgtttgaac ttcctatacg ctgatagtgc
     4861 attatcttaa aattttgggc actgttgcaa agttagcgat gaggcagcct tttgtcttat
     4921 tcaaaggcct tacatttcaa aaactctgct taccaggcgc atttcgccca ggggatcacc
     4981 ataataaaat gctgaggcct ggcctttgcg tagtgcacgc atcacctcaa tacctttgat
     5041 ggtggcgtaa gccgtcttca tggatttaaa tcccagcgtg gcgttgatta tccgtttcag
     5101 tttgccatga tcgcattcaa tcacgttgtt ccggtactta atctgtcggt gttcaacgtc
     5161 agacgggcac cggccttcgc gtttgagcag agcaagcgcg cgaccatagg cgggcgcttt
     5221 atccgtgttg atgaatcg
//
LOCUS       DAETPZ010000179         5151 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.181, whole genome shotgun sequence.
ACCESSION   DAETPZ010000179 DAETPZ010000000
VERSION     DAETPZ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.181"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            190..447
                     /locus_tag="K0W59_004028"
                     /pseudo
     CDS             190..447
                     /locus_tag="K0W59_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="K0W59_004029"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="K0W59_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HBI3024820.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="K0W59_004030"
     CDS             complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="K0W59_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HBI3024821.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="K0W59_004031"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="K0W59_004031"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBI3024822.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNLISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="K0W59_004032"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="K0W59_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3605..4420)
                     /locus_tag="K0W59_004033"
     CDS             complement(3605..4420)
                     /locus_tag="K0W59_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3024823.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            4517..>5151
                     /locus_tag="K0W59_004034"
     CDS             4517..>5151
                     /locus_tag="K0W59_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBI3024824.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     KVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1375 a         1174 c         1225 g         1377 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagatc atcaatagtc agggaaggaa gttgcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt tcaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatga
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgagat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa atacccggac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtggcagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aatcatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag
     3781 aaataacgtg catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata
     3841 agcgcatgag tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca
     3901 gatacccttg gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc
     3961 aagggagttc aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga
     4021 gggctgaaaa acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa
     4081 ttgcaattag taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca
     4141 gcgtttcact ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa
     4201 caaacttatg cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga
     4261 cgctttctcc agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga
     4321 gatgtttatg ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt
     4381 gatgccctgg tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca
     4441 gaaatcatca aataagccat gttaaatttt aaatatgacg tatgcaagtt tctggatata
     4501 tacggtgtaa agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg
     4561 tattcttttg acaggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca
     4621 gacgacggat gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg
     4681 ttatatttct gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct
     4741 gcgtctcgat gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag
     4801 tagcaaagcc aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca
     4861 aagtgccagt gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac
     4921 tgaacgttat gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc
     4981 caggtttgac taccagaagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt
     5041 ggagaagcaa cggctagcag ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga
     5101 agaagttcag gccgctttga ctcaggcact actggatctg gaatataccc t
//
LOCUS       DAETPZ010000180         5144 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.182, whole genome shotgun sequence.
ACCESSION   DAETPZ010000180 DAETPZ010000000
VERSION     DAETPZ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.182"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..253
                     /locus_tag="K0W59_004035"
                     /pseudo
     CDS             1..253
                     /locus_tag="K0W59_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..1492
                     /locus_tag="K0W59_004036"
     CDS             746..1492
                     /locus_tag="K0W59_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBI3024825.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1507..1929
                     /locus_tag="K0W59_004037"
     CDS             1507..1929
                     /locus_tag="K0W59_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBI3024826.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGKFQEAVL"
     gene            1987..2343
                     /locus_tag="K0W59_004038"
     CDS             1987..2343
                     /locus_tag="K0W59_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024827.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            2436..2654
                     /locus_tag="K0W59_004039"
     CDS             2436..2654
                     /locus_tag="K0W59_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HBI3024828.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            2656..3018
                     /locus_tag="K0W59_004040"
                     /pseudo
     CDS             2656..3018
                     /locus_tag="K0W59_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3018..3254
                     /locus_tag="K0W59_004041"
                     /pseudo
     CDS             3018..3254
                     /locus_tag="K0W59_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3447..3683
                     /locus_tag="K0W59_004042"
                     /pseudo
     CDS             3447..3683
                     /locus_tag="K0W59_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207968.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3683..4048
                     /locus_tag="K0W59_004043"
     CDS             3683..4048
                     /locus_tag="K0W59_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HBI3024829.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            4503..4658
                     /gene="hokD"
                     /locus_tag="K0W59_004044"
     CDS             4503..4658
                     /gene="hokD"
                     /locus_tag="K0W59_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBI3024830.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT         1407 a         1087 c         1421 g         1229 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgccagatac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gccaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc
      961 gtttgcgaat taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggcgaaatc
     1021 catagctgac gaactgatga caggctgtac gaattttgtg ttcagcggta agcccggcac
     1081 cggaaaaaat catcttgcag ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt
     1141 gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gagagctacg acaacggcaa
     1201 atccggtgaa aaatttttac aggagctttg tggtgttgac ctgctggtcc tggatgaaat
     1261 tggcatgcag cgggatacga aaaacgagca ggtggtactg aaccagattg ttgatcgccg
     1321 gacggcatcg ttacgcggtg tggggatgct gacaaatatt aaccatgcag cgatgaatac
     1381 acttctcggc gagcgggtga tggatcgcat ggtcatgaac ggcgggcgct gggtgaattt
     1441 taactgggag agctggcgtc cgaatgttag ccattcgagg gttgttaagt agtttcagga
     1501 ggatttatgg cgaaaccttt tactcccgaa cagcgggaag aactgaagac gcgaattgtg
     1561 gaactcgtgc atcaggacgg tcgggtcacg attcggcagt tgtcagatga aacaggtatc
     1621 agtcgtgcgt ctgtcggtcg cttatgcatg gaactggtcg caagtggtga tgtatataat
     1681 tctggctacg gggtatttcc ctctgaacag gctcataaag actggcaaaa cgcccgcaaa
     1741 aagctatcga gggtaaaggt gaagaaaccg gttgtggttg atcctgacct tatctggtca
     1801 ttaccagacg gagaaatacg ccgctacgac aggcgcctga atataatctg tcgcgagtgc
     1861 cggaagagcg aagctatgca gcgcgtgctg gcgttttatc aggggaaatt tcaggaggcg
     1921 gtactgtgag taaaattagc tatcaggctt caattaccgc tggtattcgc atcaaaggag
     1981 aggagcatgg aaataaaacc agaagatgag ttaagcaata tcgttttatt tccggtaaaa
     2041 gaggatgacc cacgtaatca ggttaatttt ctttatgaac catcggaaag accatattgc
     2101 catcacgcct ctgtccgggt tgacgaaaaa gagcgtcagg tccgctgtaa aatctgcggt
     2161 gcggttgtgg agccgtttga ctggatgctc tctgtggcaa aaagagaaac cagactggca
     2221 gatgatgtaa ggctattgcg ccaggaggaa caggaaaggc ggagaaatat agaaaagctg
     2281 atacagattg agcgtaacgc gaaagcgcgg atacgcaggg cgacaaaatc cagaactgaa
     2341 taattaaatt tagcactgtt aaaaatttaa tccttaaccg gagggatttc tgcaccctca
     2401 aatcatcagg agaccgcccg aaagggcggt aatgaatggt tacattattt agaaaaaaat
     2461 atccgcgaaa gagtagaaca acagaattcc tgtttctcat tctgtttatc gtgttgatga
     2521 caccgatatc cccgctaatt tttgtctgga taatcggaaa aataattgag ccagttattg
     2581 aattgtataa cgacgtggta tgggcgtcat tcaacacact gcacaataaa attaatccgt
     2641 ataaggaaag ctgatatggc actgacgaaa aaacaacgtg cagaactgcg catgaagttc
     2701 ggtggtcgct gcgcttattg cggctgcgaa cttggcgaaa agtggcatgc agaccatgta
     2761 aaaccggtca ttcgttttga tggaaatatg cttcaccagg aacgtgacga tatatccaac
     2821 atggtaccag aatgccaccc atgcaatctg cacaagcatt gcagtagcct agaagattat
     2881 cgacgaatta tcagtgatgg tcgtcgtgaa ttccttgcgt ccgggaaagg caaagcgctg
     2941 gttcgtatgg gattggttga aatgaaatct gacccggttg tgttctggtt tgaaaaatat
     3001 caagaagggg ctacggcatg gccactatta ccaaagagcg actgcaatgg ctggctaaca
     3061 tttctggccg cgatgatatt gacgatatag acggcggtga aattcgtgaa ctggcgctta
     3121 tcgctctggc atcactggaa gcagagcctg tagcggaatg cattgttgaa gatgggggca
     3181 tgtgtattga cgggttcggt gagtatgtgg gtcactcgct gcctgatgga atgcatgagc
     3241 tttatgctgc cccgcgaatg cgacaacctg atggatggaa agccgtagct gtagcgtgga
     3301 aggtgacgtt tactcaagtt gaccaggaat ctaatacgtt cactgctata tattttgaca
     3361 aagcggaagt tgagcgttgg gtacgactgc ataaagcatg tgattttcgg gcagatataa
     3421 caccgcttta cgcagcgccg gcagtgccgg ttgcagtaaa cgacgacatg gcttacgcat
     3481 tccatcacgc actgtcagat tcatcgcttg gctctggtga aatcgaagaa attaaaaccg
     3541 gtttgcgtgc tgcctttgcc aacgtcacta tccaaccaga gccgatagtg ccggatgaaa
     3601 tcgggccaaa cgatagtaat acgtttgatt atgttgatgg ctggaacgcc tgccgcgctg
     3661 ccatgcttaa gggagataaa taatgattaa tcgaaccaaa ctggagcaca ttctcgaata
     3721 tgccaggcag cagaaatgta ttgggcaact ttgtaaaatt ccaccaggag atatggttga
     3781 aatcgtggaa atggccatgc gtaaggctgg caactctccg gtaactctgg ctggctggat
     3841 aagctgtagt gatgcagttc ctgctgaata ttgcgatgtg attcttctcg atgatctcgg
     3901 gaatgtattc cccggttcct gggataaggt tttttgcccc actcgtggcg ggaataagat
     3961 ggcttttgtg gacaaagacg gcgttgaagt agagagctca actcactgga tgccgctacc
     4021 ggaaccaccg caggaggtga atcaatgacc tggcctgagg cattcaccac ggtaggaatt
     4081 gcgatggcgg tggcgctggt ggtgtattcg atttgccgtt ggggttaaca aacaaaaacc
     4141 ccggattgat ggtccggggt ttttgaagga aacaaaacag aaacagcaat tgccgttacc
     4201 tgttgttacc atggcaagta aacgtatctc aggcgagcgc attgcgccgt tctgacgcag
     4261 ataaattagc ctggatagaa ggtgctggca ataaaaaata gcgttttctt atcggtatcg
     4321 gtaagattgc tgcgggtgct tgaggctgtc tgcctcgggc atgccactgt aaggcagaca
     4381 gagaaaagcc ccagttaaca ttacgcgtcc tgcaagacgc ttaacattaa tctgaggccc
     4441 aatctatgtc tcacaaatgt aggttagcct cttacgtgcc gaaaggcaag gagaagcagg
     4501 ctatgaagca gcaaaaggcg atgttaatcg ccctgatcgt catctgttta accgtcatag
     4561 tgacggcact ggtaacgagg aaagacctct gcgaggtacg aatccgaacc ggccagacgg
     4621 aggtcgctgt cttcacagct tacgaacctg aggagtaaga gaccaggcgg gggagaaatc
     4681 cctcgccacc tctcatgtgt caggcatcct caacgcaccc gcactaaacc cgcttcggcg
     4741 ggttttttgt tgcgtgctga atacgcaggg tgaaaaataa ccatatattt gattatatac
     4801 acaacaaaaa ataaaagtca ttgtacttgc acattaaaca atcaaatata cggcgtgaaa
     4861 taaatatttt tcagattaat atttttgtct ctatgtggat ataaccgttt gtacttataa
     4921 acctggaggc atcgtggaaa aaataaagaa actatttagt agcaaatacg cagtcatacg
     4981 tcgtgatgac ctgtcagtta tagtcgaaat ggattacttc cctgaaaccc caaaatcaat
     5041 aatgtatcgt aatggtcgaa aggcaatttt tttaccgatg agggtaagtg acattatggg
     5101 aaatgataaa ctgctggatg aattgcgagt cagagcatcc tgtt
//
LOCUS       DAETPZ010000181         5126 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.183, whole genome shotgun sequence.
ACCESSION   DAETPZ010000181 DAETPZ010000000
VERSION     DAETPZ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.183"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..236
                     /locus_tag="K0W59_004045"
     CDS             <1..236
                     /locus_tag="K0W59_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024831.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
     gene            276..816
                     /locus_tag="K0W59_004046"
                     /pseudo
     CDS             276..816
                     /locus_tag="K0W59_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            883..2301
                     /gene="dcm"
                     /locus_tag="K0W59_004047"
     CDS             883..2301
                     /gene="dcm"
                     /locus_tag="K0W59_004047"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HBI3024832.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHVVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2282..2752
                     /gene="vsr"
                     /locus_tag="K0W59_004048"
     CDS             2282..2752
                     /gene="vsr"
                     /locus_tag="K0W59_004048"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HBI3024833.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(2741..3661)
                     /gene="yedA"
                     /locus_tag="K0W59_004049"
     CDS             complement(2741..3661)
                     /gene="yedA"
                     /locus_tag="K0W59_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HBI3024834.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGEIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            3834..4751
                     /locus_tag="K0W59_004050"
     CDS             3834..4751
                     /locus_tag="K0W59_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HBI3024835.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAAWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            4830..5012
                     /locus_tag="K0W59_004051"
     CDS             4830..5012
                     /locus_tag="K0W59_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HBI3024836.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
BASE COUNT         1252 a         1225 c         1464 g         1185 t
ORIGIN      
        1 gggctttcta tacatggtgt ggctatttcg tctgggcaat ggcgcgatac atctgggtga
       61 tgagccggat ccccgatgcc cccgtgtcgg gatttgaaag tgatttagga tctaccgcgg
      121 ggaagtggtt aggagcattg gtcggatttc tgtttatggc cctggttggt gccttgttag
      181 gcggcattgc atggtacacc cgacctcgtc ccgcacgttg cagacgctat gaataaaaac
      241 tgctaaggtt gcgactcttt ggcaacggag cgcgtatgga gttacaacac tggcaggcac
      301 agttcgagaa ctggttgaaa aaccatcatc aacatcagga cgcggcgcat gacctgtgtc
      361 attttcgccg tgtctgggct acggcacaga aactggcggc agatgacgat gtcgatatgc
      421 tggtgatttt aaccgcctgt tattttcacg atatcgtaag cctggcgaaa aatcatccgc
      481 aacggcagcg ttcttcaatc ctggcggcag aagagacgcg tcgcctgctc cgtgaagagt
      541 tcgtgcaatt tccggcggag aaaattgagg ccgtttgtca tgccattgcc gctcacagtt
      601 tcagcgcgca aatcgccccc ttaacaacgg aagctaaaat cgtccaggat gcggatcggc
      661 tggaagccct gggggcgatt ggcctggcgc gcgtatttgc cgtttcaggg gcattgagct
      721 tggcgctgtt tgatggcgaa gctccgtgcc gaactggcgg gggagaatga aggtatcgat
      781 cacaaggtga tagatgcgtt ttcacccgct ggctgagcgc gtggccctaa acggcagtaa
      841 ttatgttaac ctgtcggcca tctcagatgg ccggttaaat ctatgcagga aaatatatca
      901 gtaaccgatt catacagcac cgggaatgcc gcacaggcaa tgctggagaa actgctgcaa
      961 atttatgatg ttaaaatgct ggtggcgcag cttaatggtg tgggtgagaa tcactggagc
     1021 gcggcaattt taaaacgtgc gctggcgaat gactcggcat ggcaccgttt aagtgagaaa
     1081 gagtttgccc atctgcaaac gttgttaccc aaaccaccgg aacatcatcc gcattatgcg
     1141 tttcgcttta tcgatctatt tgccggaatt ggcggcatcc gtcgtggttt tgaatcgatt
     1201 ggtggacagt gcgtgtttac cagcgaatgg aacaaacatg tggtacgcac ttataaagcc
     1261 aaccattatt gcgacccggc gacgcatcat tttaatgaag atatccgcga catcaccctc
     1321 agtcatcaag aaggcgtgag tgatgaggcg gcggcggaac atattcgtca acacattcct
     1381 gaacacgatg ttttactggc cggtttccct tgtcagccat tttcgctggc tggcgtatcg
     1441 aaaaagaact cgctcgggcg ggcgcacggt tttgcctgcg atacccaggg cacgctgttc
     1501 tttgatgtgg tgcgcattat cgacgcgcgt cgtccggcga tgtttgtgct cgaaaacgtc
     1561 aaaaacctga agagtcacga taagggaaaa acgttccgca tcatcatgca gacgctggac
     1621 gaactgggct atgacgttgc cgatgcagaa gataacgggc cggacgatcc gaaaatcatc
     1681 gatggtaaac atttcctgcc gcagcaccgt gaacgcatcg tgctggtggg ttttcgtcgc
     1741 gatctgaatc tgaaagccga ttttactctg cgtgatatca gcgaatgttt ccctgcacag
     1801 cgagtgacgc tggcgcagct gctggacccg atggttgagg cgaaatatat cctgacgccg
     1861 gtgctgtgga agtacctcta tcgatatgcg aaaaaacatc aggcgcgcgg taacggcttc
     1921 ggttatggaa tggtttatcc gaacaatccg caaagcgtca cccgtacgct gtctgctcgt
     1981 tattacaaag atggcgcgga aattttaatc gatcgcggtt gggatatggc cacgggtgag
     2041 aaagactttg acgatccgct gaatcagcaa catcgtccac gtcggttaac gcctcgtgaa
     2101 tgcgcgcgct taatgggttt tgaagcgccg ggagaagcga aattccgcat tccggtttcg
     2161 gacactcagg cctatcgcca gttcggcaac tcggtggtcg tgccggtctt tgccgcggtg
     2221 gcaaaactgc ttgagccaaa aatcaaacag gcggtggcgt tgcgtcagca agaggcacaa
     2281 catggtcgac gttcacgata aggccacccg cagcaaaaat atgcgcgcga ttgccacgcg
     2341 tgatacggcg attgagaagc gcctcgccag tttgttaacc gggcagggcc ttgcgtttcg
     2401 cgttcaggac gccagtctgc ccggacgccc ggattttgtc gttgatgaat atcgctgcgt
     2461 gatatttacc cacggctgct tctggcatca tcatcactgc tatctgttta aagtgcctgc
     2521 gactcgaact gagttctggc tggagaagat aggtaaaaat gttgaacgcg atcgccgcga
     2581 tatcagtcgc ttgcaggagc tcggctggcg cgtactgatt gtctgggagt gtgcgttacg
     2641 taggcgcgag aagctgacgg atgcggcgct taccgagcgt ctggaagagt ggatctgcgg
     2701 cgaaggtgcc agcgcgcaga tcgacacgca ggggattcat ttactcgctt gatgcatcct
     2761 gaataactgg cgcgacaacg ggtttggccg ggaagagata ttttcccaac gtgaccagta
     2821 ccaccgcgaa aacaattacg ccgagcgcca gccattcaat cttcgacagc gtttcgccac
     2881 ccagtcccgt acccagcaag accgcgacca ccgggttaac gtaggcgtag ctggtggcga
     2941 gagccggact gacattacgg attaaataca tataagcgtt aatggcgata atcgaaccaa
     3001 acagcgccag atagccgacc gcaaggaagc ctgacaggga agggagcgcc gtcagttttt
     3061 cacccgcaat catcgatgcg atcattaaca ccacgcctgc cgccagcatc tcaatctcac
     3121 ccgccatcat ccctacaggt aaggtaatgc gcgagccata aactgagcca aacgcccagc
     3181 taatcgagcc gattaaaatc agaatcgcgc cccacggatt gccgcttaaa tttccaccgc
     3241 tattgagcat gatgattccg gcaagcccaa tggcaatacc cacccattcc agtttgcgcg
     3301 ttttaatgcc aaacaggcga ctgaagcaca gggtaaagag gggcacggtt gcaatcacta
     3361 cggcggcgat gccggaagga acattttgat gttcggcaac cgtcaccatg ccattaccga
     3421 cagccagcaa taacaggcca atcagcgcgg cattgagcag cggacgtagc gggggaagtt
     3481 tgtgtccgcg cagtagcaaa aatgccagca ataaaatacc ggctgccagg aatcgaacgc
     3541 ccgccatcat taacggaggc cagcttccca cgccaatccg aatgacaaaa taggttgagc
     3601 cccaaatgat atacaacgca aacagagcgc caaaaagcgg taacaactgg cggaaacgca
     3661 taatccctca cggcggaaat aaaaaggtgg ttcatagtaa acgtgaaaat cattctgctg
     3721 gcgagagata taattgcact tgattgttaa ataaatgttg acctgtgaag caagtatcag
     3781 agctgccgtt tttgcttcat acttacaccc ttcagcaata aaaatgagag ggaatgcttt
     3841 tggccgggag tagtttactg acgttgctcg atgatatcgc cacactgctg gacgatatct
     3901 ctgtgatggg caaactggcg gcgaagaaaa ccgccggtgt attaggggat gatttatcgc
     3961 tcaatgcgca acaagtttca ggcgtgcggg ccaaccggga acttcccgtg gtctggggcg
     4021 tggcgaaagg atcgctgatc aataaagtga ttctggtgcc gctggcgctg atcatcagtg
     4081 cgtttatccc gtgggcgatt acgccactgt tgatgattgg tggcgcgttt ctctgctttg
     4141 aaggggtaga gaaagtgctg catatgctgg aggcgcgtaa acataaagaa gatccagcgc
     4201 agagccagca gcgtctggag aagctggcgg cgcaggatcc gctgaagttt gaaaaggaca
     4261 aaattaaagg ggcgattcgt accgatttta ttttgtctgc ggaaatcgtc gccatcacgc
     4321 tggggattgt ggcagaggcg ccgttgctta atcaggtgct ggtgctttca ggcatcgcgc
     4381 tggtagtgac cgtgggcgtc tacggtctgg taggggttat cgttaagatt gatgacctag
     4441 ggtattggct ggcggaaaaa tccagcgcgc tgatgcaggc attaggtaaa gggttattga
     4501 ttatcgcggc ttggctgatg aaagcattat cgattgtcgg cacgctggcg atgttcctcg
     4561 tcggcggcgg gattgtggta catggtattg cgccgctgca tcacgctatt gaacatatcg
     4621 ccgggcagca aagtgcagtg gtggcgatga tattaccgac tgttttaaat ctgattcttg
     4681 gatttatcat cggcggcatc gtggtgctgg gagtgaaagc agtagcgaaa atgcgcggtc
     4741 aggcacatta atactcgcac tggctattat gcaaaattcg ccatcttcaa ctaaggtgaa
     4801 caagtttcac tcgaaaaaag gaggcgagta tgagctttat ggttagtgag gaagttacgg
     4861 ttaaagaggg cggcccgcga atgattgtca ctggatactc cagcggtatg gttgagtgtc
     4921 gttggtatga cggttacggg gtcaagcggg aagcttttca tgaaaccgat cttgttccgg
     4981 gggaggggag tcgttctgcg gaagaagttt gaaagggtat cattaacacc aggccggatc
     5041 acatccggcc tggtgtccca atgcctgcaa tgaatgatag tcgcgtctta tcatgccacc
     5101 attataaaat tatcgctgtt ttgcgg
//
LOCUS       DAETPZ010000182         5118 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.184, whole genome shotgun sequence.
ACCESSION   DAETPZ010000182 DAETPZ010000000
VERSION     DAETPZ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.184"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..368)
                     /locus_tag="K0W59_004052"
     CDS             complement(<1..368)
                     /locus_tag="K0W59_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBI3024837.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLIIKNSTIHGMIYSECMTT"
     gene            894..1868
                     /gene="hemB"
                     /locus_tag="K0W59_004053"
     CDS             894..1868
                     /gene="hemB"
                     /locus_tag="K0W59_004053"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBI3024838.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1974..2825)
                     /gene="tauD"
                     /locus_tag="K0W59_004054"
                     /pseudo
     CDS             complement(1974..2825)
                     /gene="tauD"
                     /locus_tag="K0W59_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
     gene            complement(2822..3649)
                     /gene="tauC"
                     /locus_tag="K0W59_004055"
     CDS             complement(2822..3649)
                     /gene="tauC"
                     /locus_tag="K0W59_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HBI3024839.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVQALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLLLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(3646..4413)
                     /gene="tauB"
                     /locus_tag="K0W59_004056"
     CDS             complement(3646..4413)
                     /gene="tauB"
                     /locus_tag="K0W59_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HBI3024840.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4426..>5118)
                     /gene="tauA"
                     /locus_tag="K0W59_004057"
     CDS             complement(4426..>5118)
                     /gene="tauA"
                     /locus_tag="K0W59_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI3024841.1"
                     /translation="VPIEVFLLASKLGNSEALVVKKTISKPEDLIGKRIAVPFISTTH
                     YSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDGAYVWAPAVNALEKDGKVLTD
                     SEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAIDAQQPYIANPDAWLKQPENIS
                     KLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNKAIIDTAQFLKEQGKVPAVAN
                     DYSQYVTSRFVQ"
BASE COUNT         1243 a         1409 c         1273 g         1193 t
ORIGIN      
        1 tcagttgtca tgcactcgga ataaatcata ccgtgaatag tgctgttttt gattatcaga
       61 ctattggcgt tggtattggt agaaataccg tcgtccagat agtaggtaga aatcacgccg
      121 ttaacggtag cgttattaat gaccgggtag atatcgccgt tgtaatagct atcagcggca
      181 tagttgttcc agcctacata accatcatag taaacgtcat cagcataggt ggcgtcgttg
      241 tagtgatgga aagtattgta agtttgacca gaaatatcgt tggtcgctgc attagcctga
      301 gaggtgatag ccagagtgca agccaatgct aattgtgata ctacaagttt ctttttccag
      361 gagtgcattt gtcatccctc ctcagggacg taataagttg atccatcaat tatcaatgca
      421 tagaaaacat attatgtttc gcgctaacga ttatgctgcg ttacagacga atggttcaat
      481 tattctttcc cctaagtccg aatgtggaca tatataattt atggagaaaa acacgcatca
      541 aattgataaa aggacgatat ttcagcatgt tgcagataat gttatttttg atgcatacgc
      601 gttataaata tcaaaaacac aaaccagata aatatcttat caatatacaa caaagtttga
      661 attattcaat ttatagcagc acaaccaaac gccaaaaaaa ataataacca agagctataa
      721 ataaagatta aaaatatttt cagtgaatat gaaattattg ctatttctat tacaaagatg
      781 tcctggtctc tctttcattc tcttcttgtc aacatcgcga caactttcgt aaaacactcc
      841 tgccccgctt caggtatact gtgcacctta attccaaaaa catcaggcag accatgacag
      901 acttaatcca acgccctcgt cgcctgcgca aatctcctgc gctgcgcgct atgtttgaag
      961 agacaacact tagccttaac gacctggtgt tgccgatctt tgttgaagaa gaaattgacg
     1021 actacaaagc cgttgaagcc atgccaggcg tgatgcgcat tccagagaaa catctggcac
     1081 gcgaaattga acgcatcgcc aacgccggta tccgttccgt aatgactttt ggcatctctc
     1141 accataccga tgaaaccggc agcgacgcct ggcgggaaga tggactggtg gcgcgtatgt
     1201 cgcgcatctg caagcagact gtgccagaaa tgatcgtcat gtcagacact tgtttctgtg
     1261 aatacacttc tcacggtcac tgcggtgtgc tgtgcgagca tggcgtcgac aacgacgcga
     1321 ctctggaaaa tttaggcaag caagccgtgg ttgcagctgc tgcaggcgca gacttcatcg
     1381 ccccttccgc cgcgatggac ggccaggtac aggcgattcg tcaggcgctg gacgctgcag
     1441 gctttaaaga tacggcgatt atgtcgtatt cgaccaagtt cgcctcttcc ttttatggcc
     1501 cgttccgtga agctgccgga agcgcattaa aaggcgaccg caaaagctat cagatgaacc
     1561 caatgaaccg tcgcgaggcg attcgtgagt cactgctgga tgaagcccag ggcgcagact
     1621 gcctgatggt taaacctgct ggagcgtacc tcgacatcgt gcgtgagctg cgtgaacgta
     1681 ctgaattgcc gattggcgcg tatcaggtga gcggtgagta cgcgatgatt aagttcgccg
     1741 cgctggcggg tgctatagat gaagagaaag tcgtgctcga aagcttaggt tcaattaagc
     1801 gtgcgggtgc ggatctgatt ttcagctact ttgcgctgga tttggctgag aagaagattc
     1861 tgcgttaatt tttttcctct cagaaagggg gagttaacct gttgaaagta cggtgaacgc
     1921 cccctcaccc taaccctctc cccaaggggg cgagggaacc gtccacactc gtatcacccc
     1981 gcccgataaa acggtttatc cccaaggatc gtcgcccgat gcataatccg ccgctgtggc
     2041 aggtaatcgg cattggcata gtgctgagtc acgcggttat cccaaatcgc aatatcattc
     2101 ggttgccagc gccaacgcac ctgaaactcc ggtttagtga tatgggcgaa taaaaagcct
     2161 aacaaggctt cgctctcttt ctcgctgaca tcgacaattc gcgtagtaaa gccttcattc
     2221 acaaacagcg cctgtttacc gctcaccgga tgcgttcgca ccaccggatg tagcaacgga
     2281 ggatttttcg cgaccgcctc gcgccagcgt tgatgttcct cctcggtttt gcggtatttg
     2341 tattccggga atgatttacg gaaatcatgc tccgcacgca gccagctcag cagctggcgg
     2401 aagggaacag agagcgcctc ataggccgca ataccgctgg ttcagagcgt atcaccgccg
     2461 gtcgaaggta actctttagc tgccagaatc gcccctgcgg gtggcgtttc aataaatgtc
     2521 acatcggtat gccagttgtc gttatccggc ggattatcgt tatgggtatc cagcacgatg
     2581 atctcgtcaa ccccttcggc atgagggtaa acagggtgaa tatgcagttc gccaaaacgc
     2641 tgggccagcg cgcgttgctg ctgcggcgta atagcttgat cgcgcagaaa taccacctga
     2701 tggcgcagca ccgcatggta aagctgttca aactgattat cgcttaacgg gcgcgtcagg
     2761 tcggcacccg aaatttgtgc gccgatatgc ggccccagcg gggtaatgct cagacgttca
     2821 ctcattgtac ttctccatgc cagggcgtca gacggcgctg taacgcgcgc agacccagtt
     2881 ctaaaagaaa ggcgataatc gcaatcaccg cgatccccgc cagtaccacg tcagtcgcga
     2941 gaaattcacc cgctgactga accataaatc ctaaaccgcg tgtcgcggca atcagctccg
     3001 ccgctaccag cgtagaccag cccacaccca gaccaatgcg taatccggtg aggatttccg
     3061 gcagcgcgcc gggcaaaatg acaaaccaca gcacctgcgc acggctggca cccagcgact
     3121 gggcggcacg aatgcgaacc tgctgcgcgc ttttcacccc tgccagcgcc gacatcgcca
     3181 ccggcgcaaa aatcgctaaa tagatcagta agatcttcga ggtttcacca ataccaaacc
     3241 agatcaccat cagcagcaaa taagccagcg gtggcaccgg acgataaagt tcgattaccg
     3301 gatccagaat gccgcgcacc gtcgggctta gccccatggc gatcccgacc ggaataccga
     3361 tcaccaccgc cgcgagtagc gcctgcacaa tgcgcgtcag gctggctgcc agatgctgcc
     3421 acagcgtggc gtccataaat ccctgcggtc cggcaatggt gagcagtttt gccagtactt
     3481 gttgcggcgg cggcaaaaac agcgggctaa tcagttgcag cgccgccacc gtccaccata
     3541 cagtgagtaa aaccgctaac gtgccaatgc ttaaggtcac ctgacgcgag agcggccagc
     3601 gccatttcag ccgccgcgaa tgcagttttt cattaatgag cacactcatg agaacgcctc
     3661 ccgttgctca aatacgcggc ttaaaacata ttcgcgcatg gcgatgaatt gtggatcgga
     3721 cttgatgctg cggctcgact ctcccgcaac aaagcggcga gcaaagttga gcggcagccg
     3781 ctccagcaca cggccagggc cggatgaaag cagaaccagt tcagtcgcca taaacaccgc
     3841 ttcttctata tcgtgggtaa tcaacagcac ctgcttgccc gtctcctgcc agagtttcag
     3901 cagcagagtt tgcatctggt cgcgggtgaa ggcgtccagc gcaccaaacg gttcatcgag
     3961 taataacagc tggggattcg ccgccagcgc gcgggcaatc cccacccgct gacgttgacc
     4021 atcggaaagc tgccagatat agcgtttttc tgcgccttcc agccccactt ttttcagcat
     4081 ctggtgcgcg atttccagtc gctgcatttt ctctatacct gccaattgca ggccgaacgc
     4141 cacgttgtcc tgtacattgc gccacggtag cagcccttca ttctgaaaaa ctacgccacg
     4201 ctctgctccc ggtccctcaa tacgcttacc cgccagttga atgctgccat gctgataagg
     4261 cacaaaaccg gcaatcagat tcagcagggt ggttttaccg cagccggacg gccccagcac
     4321 caccagtagc tcgccgcttt ccagcgtcag gttgatatct tccagtgccg gtttgccgcc
     4381 ataatcggca tagagatgag agatttgcag catctgcgcc tccttttatt gcacgaagcg
     4441 cgaggtaacg tactggctgt aatcattcgc tacagccggg accttgccct gctctttcaa
     4501 aaactgcgcg gtgtcgatga ttgctttgtt caccggtccg gtcagttctg ccgtttgttg
     4561 ctgcggcgtc agataggtat ttcccttcac cagccccgga acgtcacctt caggcacgcc
     4621 gcttaaacgc gccagtttgc tgatgttttc cggctgtttc agccacgcgt cagggttagc
     4681 aatgtacggt tgctgtgcat cgatggcgct tttggcgaac gctttcacga cctcaggatg
     4741 tttctcggca aaatctttgc gcaccaccca gacgtccagc gttggcgcac cccactgccc
     4801 gacctgttca gaatcggtca gcaccttgcc gtctttttcc agggcgttaa ccgccggtgc
     4861 ccagacataa gcaccatcaa tatcaccccg ctgccatgcg gcgataatcg cgggcggctg
     4921 caggttcaca atctccacta gccctggttt aatcccccag tgtttcagcg ccgccagcag
     4981 gctgtagtgg gtggtggaga taaacggtac ggcaatgcgt ttaccaatca aatcttcggg
     5041 tttgctgata gttttcttta ccaccagcgc ttcggagtta cccagttttg acgccagcaa
     5101 gaagacttca atcggcac
//
LOCUS       DAETPZ010000183         4992 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.185, whole genome shotgun sequence.
ACCESSION   DAETPZ010000183 DAETPZ010000000
VERSION     DAETPZ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.185"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            91..1206
                     /gene="adrA"
                     /locus_tag="K0W59_004058"
                     /gene_synonym="dgcC"
     CDS             91..1206
                     /gene="adrA"
                     /locus_tag="K0W59_004058"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HBI3024842.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(1223..2032)
                     /gene="proC"
                     /locus_tag="K0W59_004059"
     CDS             complement(1223..2032)
                     /gene="proC"
                     /locus_tag="K0W59_004059"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBI3024843.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRVQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            2152..2610
                     /locus_tag="K0W59_004060"
     CDS             2152..2610
                     /locus_tag="K0W59_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBI3024844.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2793..3317
                     /gene="aroL"
                     /locus_tag="K0W59_004061"
     CDS             2793..3317
                     /gene="aroL"
                     /locus_tag="K0W59_004061"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HBI3024845.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPNQV
                     ISEIRSALAQTINC"
     gene            3367..3558
                     /gene="yaiA"
                     /locus_tag="K0W59_004062"
     CDS             3367..3558
                     /gene="yaiA"
                     /locus_tag="K0W59_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HBI3024846.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            3816..4493
                     /locus_tag="K0W59_004063"
     CDS             3816..4493
                     /locus_tag="K0W59_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HBI3024847.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            4565..4849
                     /gene="ppnP"
                     /locus_tag="K0W59_004064"
     CDS             4565..4849
                     /gene="ppnP"
                     /locus_tag="K0W59_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBI3024848.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT         1213 a         1186 c         1315 g         1278 t
ORIGIN      
        1 gctaacgggt gagctacgaa agtggctcac cctaaatttc atacttctgc ctttagcccc
       61 gtctctataa tttgggaaaa ttgtttctga atgttcccaa aaataatgaa tgatgaaaac
      121 tttttcaaaa aagcggcggc gcatggggag gaacctcctt taactcctca aaacgaacat
      181 cagcggtccg ggctgcgctt cgcccgtcgc gtcagactac cccgtgcggt tggcctggct
      241 ggcatgttct taccgattgc ttcaacgctg gtttcgcacc cgccgccggg ctggtggtgg
      301 ctggtgttgg tcggctgggc gttcgtctgg ccgcatttag cctggcagat agcgagcagg
      361 gccgtcgatc cgcttagccg ggaaatttac aacttaaaaa ccgatgcagt attagcggga
      421 atgtgggtag gcgtaatggg cgtaaacgtg ctgccttcca ccgctatgtt gatgattctg
      481 tgtctgaatt tgatgggggc aggcggcccc cgtctgtttg tcgcgggtct ggtgttgatg
      541 gtggtttcct gccttgtcac cctcgagctt acgggcatta ccgtgtcgtt caatagtgcg
      601 ccgctggaat ggtggctctc ccttcccatt attgtcgttt atcctctgct gtttggctgg
      661 gtcagctacc agacggcaac caaactggcg gaacataaac gcaggttgca ggtcatgagt
      721 actcgcgacg gcatgacggg cgtgtataac cgacgtcatt gggaaactat gttacgcaat
      781 gaatttgata actgtcggcg gcataatcgc gatgcaacgt tactgattat cgatatcgac
      841 catttcaaga gcatcaacga tacctggggt catgatgtgg gcgatgaagc gattgtggcg
      901 cttacccgac agttacaaat taccctgcgc ggtagcgatg tgattggtcg gtttggcggc
      961 gatgagtttg cagtaatcat gtccggtacg ccagctgaga gcgccattac cgccatgtta
     1021 cgggtgcgtg aagggctaaa cacgttacgc ttgccgaata cgccacaggt aactttacgg
     1081 attagtgtgg gggttgcgcc gctgaaccca caaatgagtc actatcgtga gtggttgaaa
     1141 tcggcagatt tggcgcttta caaagcaaag aaagccggac gtaaccgcac cgaagtggcg
     1201 gcctgacgtc cggcgaaagt catcaggatt tgctgagttt ttctgatttt tccatacact
     1261 tcgtcatcgc ttcgatcact gcggcacgga agcctttctc ttccagtacg cgtaccgctt
     1321 caatggtggt acctcccggt gagcagacca tatctttcag tgcccccgga tgttctcccg
     1381 tttccagcac catttttgcg gaacccatta ccgcctgagc ggcaaattta tacgcctgga
     1441 cgcgtggcat cccgcccagc acggcggcgt cggccatcgc ttcgataaac ataaatacgt
     1501 aggctggcga agaaccgctc acaccgacca ccgggtggat catcggctca gcaattactt
     1561 ccgcttcgcc aaagcagcgg aaaatattca gcacatcagc ggtatcttct ggggttacca
     1621 gcgcgtttgg cgttacggag gtcatcccgg cattaaccag cgcgggagtg ttcggcatgg
     1681 cgcggataat tttccggtca tggcccagcg cgcgggcaag ctggtcgagc gtgacacctg
     1741 cagcaataga aacgaccaga gagtctttat tcaggctgga ggtgatttcg ctaagcactt
     1801 taatcatgat gccaggttta acggcagcaa aaatgatgtc ggcgatttgc gccacttctt
     1861 gcgccgattc tgcggcgttg atgccgaact ggtcatgcag ggcggcgact ttatccgggg
     1921 agggggtgta tacccagatt tgccctggaa gcacctgacc gctggcaatc agaccgccga
     1981 gaatggcttt tcccatattg ccgcagccaa taaaaccgat tttcttttcc attgcctcac
     2041 tcctgccgtg aaattcattg ttttgataat cgctggcaga agcataaaca gaactatgcc
     2101 ggaaggcaaa agcgcgacac aatagaggat tacccaacaa aggatgactt tatgacaatt
     2161 tgggtggatg ccgacgcgtg tcccaatgta attaaagaga ttttgtatcg cgcggcggaa
     2221 cgtatgcaga tgccgctggt actggtagca aaccagagtt tacgcgtgcc gccatcgcga
     2281 tttattcgta cgctgcgcgt cgcggcaggt ttcgacgttg ccgataacga aattgtccgg
     2341 cagtgtgaag cgggcgattt ggtgatcacc gcagatatac ctttggctgc tgaagccatc
     2401 gagaaaggcg cagcggcgct taatccgcgc ggcgaacgtt acacgccagc gaccattcgt
     2461 gagcgcctga cgatgcgcga ttttatggat accttacgtg ccagtgggat ccagaccggc
     2521 ggaccagata gcctttcaca acgtgaccgc caggcctttg ccgcggagct ggagaagtgg
     2581 tggctggaag tgcaacgtag tcgtggctaa atgtaattta ttatttacac ttcattcttg
     2641 aatatttatt ggtatagtaa gggatgtatt gagattttca ctttaagtgg aattttttct
     2701 ttacaatcga aattgtacta gtttgatggt atgatcgcta ttctcatgac accggctttc
     2761 gccgcattgc gacctattgg ggaaaaccca cgatgacaca acctcttttt ctgatcgggc
     2821 ctcggggctg tggtaaaaca acggtcggaa tggcccttgc cgattcgctt aaccgtcggt
     2881 ttgtcgatac cgatcagtgg ttgcaatcac agctcaatat gacggtcgcg gagatcgtcg
     2941 aaagggaaga gtgggcggga tttcgcgcca gagaaacggc ggcgctggaa gcggtaactg
     3001 cgccatccac cgttatcgct acaggcggcg gcattattct gacggaattt aatcgtcact
     3061 tcatgcaaaa taacgggatc gtggtttatt tgtgtgcgcc agtatcagtc ctggttaacc
     3121 gactgcaagc tgcaccggaa gaagatttac ggccaacctt aacgggaaaa ccgctgagcg
     3181 aagaagttca ggaagtgctg gaagaacgcg atgcgctata tcgcgaagtt gcgcatatta
     3241 tcatcgacgc aacaaacgaa cccaaccagg tgatttctga aattcgtagc gccctggcac
     3301 agacgatcaa ttgttgattt tagagcgcct atacttaacg ttcatcccgt gaaataagga
     3361 agaacgatgc caacgaaacc gccttatcct cgtgaagcat atatagtgac gattgaaaaa
     3421 ggaaagccag gacagacggt aacctggtac caactcagag ccgatcatcc taaaccagac
     3481 tcgttgatca gtgaacatcc gacggctcag gaagcgatgg atgcgaaaaa acgctatgag
     3541 gaccctgaca aagagtgacc gcatcagact gctcggaagg gattctgagt gccactacaa
     3601 gggatctgcg tcacattttt cataattcat gtttttctaa taattagaat attaaacaat
     3661 aacattccat tactggaatc atttggaatc tttacattat gccgtgcacg tctgctgcta
     3721 cgctttttgt catttgtagc acaagtaagt gtcagcagtg gtgcttcaca cttgcccggt
     3781 aattaacgac gaaagaaaag taaggtggat gaacaatgag tgcgtcgttg gcgatcctga
     3841 ccatcggcat tgtacctatg caggaggttt tgccgctcct gacggaatac attgacgaag
     3901 ataatatttc ccatcatagc ctgctgggga agttaagtcg tgaagaagtg atggcggagt
     3961 acgcgccaga agcaggcgaa gacaccattc tcacattatt aaatgacaac cagctggccc
     4021 atgtttcgcg tcgtaaagtg gagcgagatc tgcaaggtgt ggttgaagtg ctcgataatc
     4081 agggttatga cgtcatttta ttaatgagta cagcaaacat tagtagtatg actgcgcgta
     4141 atacgatctt tcttgagccg tcgcgaatat tgcctccact ggtttcctct attgttgaag
     4201 atcatcaggt gggggttatc gttccggttg aggagatgct gcccgttcag gcgcaaaaat
     4261 ggcaaatttt gcagaaatcg ccggtatttt cattgggtaa ccccattcat gattcagaac
     4321 aaaaaatcat tgatgctggg aaagaattac tggcaaaagg ggctgatgtc atcatgctgg
     4381 attgtttagg atttcaccaa cgtcatcgcg atttactgca aaaacagctc gatgttcctg
     4441 tcttgctctc taacgtattg attgcacggc tggctgcgga attactggtg taattttgcg
     4501 tgacagccag cgcctctggc ccctatagtg aagtagatgt tcaactacca gacagggcca
     4561 gtttatgctt caaagtaatg agtacttttc cggcaaagtg aaatcgatcg gcttttccag
     4621 cagcagcact ggtcgcgcca gcgtgggtgt tatggttgaa ggcgaataca ccttcagcac
     4681 cgctgagccg gaagagatga cggtaatcag tggcgcgctg aatgtgttac tgcctgacgc
     4741 gaccgactgg caagtgtatg aagccggttc ggtgtttaat gttcccggtc acagtgagtt
     4801 tcatcttcaa gttgccgaac ctacctctta tctgtgccgc tatctgtaat tcctcgcctt
     4861 ccccttgaac gggagggcat ttttctgaaa tatcctctct ttaacccata ataatatttc
     4921 ctttgctgcg attttttcaa tttccgatat attcataatt catcaaggtt gatataaata
     4981 tcagtgaaga tc
//
LOCUS       DAETPZ010000184         4952 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.186, whole genome shotgun sequence.
ACCESSION   DAETPZ010000184 DAETPZ010000000
VERSION     DAETPZ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.186"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..611
                     /locus_tag="K0W59_004065"
                     /pseudo
     CDS             1..611
                     /locus_tag="K0W59_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
     gene            632..1292
                     /gene="csiR"
                     /locus_tag="K0W59_004066"
                     /pseudo
     CDS             632..1292
                     /gene="csiR"
                     /locus_tag="K0W59_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
     gene            complement(1293..1742)
                     /gene="kbp"
                     /locus_tag="K0W59_004067"
     CDS             complement(1293..1742)
                     /gene="kbp"
                     /locus_tag="K0W59_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HBI3024849.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(1826..1984)
                     /locus_tag="K0W59_004068"
     CDS             complement(1826..1984)
                     /locus_tag="K0W59_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HBI3024850.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            2167..2466
                     /locus_tag="K0W59_004069"
     CDS             2167..2466
                     /locus_tag="K0W59_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3024851.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            2476..3000
                     /gene="ygaP"
                     /locus_tag="K0W59_004070"
     CDS             2476..3000
                     /gene="ygaP"
                     /locus_tag="K0W59_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HBI3024852.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKIPWNQRA"
     gene            complement(3047..3451)
                     /gene="stpA"
                     /locus_tag="K0W59_004071"
     CDS             complement(3047..3451)
                     /gene="stpA"
                     /locus_tag="K0W59_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HBI3024853.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            4119..4556
                     /gene="alaE"
                     /locus_tag="K0W59_004072"
     CDS             4119..4556
                     /gene="alaE"
                     /locus_tag="K0W59_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HBI3024854.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKGWIKNLADILAYVTFQSPVYVAILLVVGAD
                     WHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(4593..4943)
                     /locus_tag="K0W59_004073"
     CDS             complement(4593..4943)
                     /locus_tag="K0W59_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HBI3024855.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERQRHEHYGIPHGFSSRV
                     ALERYLNGLFGEAS"
BASE COUNT         1261 a         1168 c         1165 g         1358 t
ORIGIN      
        1 tgccggggct gaaagccgtt ggatcttatc gctcggttct ggaattgctc aatattcccc
       61 atgcgaaatt aatcatggac tgtgtgatat tactttccgt aaccagttgc ctgaactcgg
      121 ccctgtatac cgcctcaagg atgctctact ccttaagccg tcgcggtgat gctcccgcgg
      181 taatgggtaa aatcaaccgc agtaaaaccc cgtatgtggc ggtgttactc tccaccggcg
      241 cagcattctt aacggtggtg gtgaactatt acgcgcctgc gaaggtattt aaatttctga
      301 tcgacagctc cggcgctatc gccctgctgg tttatttagt catcgccgtt tcacagttgc
      361 ggatgcgcaa aattctgcga gcagaaggaa gcgaaattcg cttgcgaatg tggctttatc
      421 cctggctcac ctagctggtc atcggcttta ttacctttgt gttggtagtg atgctattcc
      481 gtccggcgca acagttagaa gtgatctcca ccggcttatt agcgataggg attatctgta
      541 ccgtgccaat tatggctcgc tggaaaaagc tggtattgtg gcaaaaaaca cccgttcata
      601 atacgcgctg ataatgatca ggagtcacac catgaccatt acgtctctgg atggctatcg
      661 ctggctgaag aacgatatta ttcgcggtaa ttttcaaccg gatgaaaaat tacggatgag
      721 tttactgaca tcgcgttatg cacttggcgt tgggccgttg cgggaagtac tttctcaact
      781 ggtggtggaa cggctggtca cggtggtgaa tcaaaaaggg tatcgggtgg cgtctatgtc
      841 agagcaggag ctgctctgac atagacgccc gcgccaatat ggaagcgatg ctggtcagtc
      901 tggcgattgc ccgcggtggc gatgaatggg aggcagacgt tctcgcaaaa gcgcatctgc
      961 tcaataagct ggaagcctgt gacgccagcg agaaaatgct tgatgagtgg gatctgcgtc
     1021 atcaggcgtt tcatacagca attgtggcgg gctgtggttc tcactatttg ctgcaaatgc
     1081 gtgaacggtt gtttgatctg gcggcgcgtt atcgatttat ctggctgcgg cgaacggtgc
     1141 tttcggtgga aatgctggag gataaacacg atcagcacca gaccctgact gcggcggtac
     1201 tggcgcgaga tacagcgcgc gccagtgagt taatgcgcca gcatttactg acgccaattc
     1261 ccattatcca gcaggcgatg gctggcaatt aactactctt ccggaatacg caacacttgc
     1321 cccggataaa ttttatccgg gctttttagc atcggtttat tcgcttcgaa gattttattg
     1381 tacagattag cgttaccgta gacctgtttg gaaatggcac tcagagtgtc gccagactta
     1441 acggtataaa actggctggc agtggctggt gtcgccgttt tcacctgatc atcaacactg
     1501 gcaataccgg aaatattccc caccgcaaca aggattttct ccttcgcctc ctgactcagg
     1561 ccgtcaccag tgaccgtcgc tttgccgtcg gcaatttgaa tgttcacttt atcggcatca
     1621 ggtataccgg ttttgctcag atgctcctgc accttcttcg cctgatcgtc tttatcgtgc
     1681 tgacctgtaa ccgcatccca gagtttttct ccggcatctt tcacaaaatt gaacagaccc
     1741 atagctacct cattcgttaa cggaaacact cagaaagtgt agcagaggaa cgcggcgcgt
     1801 taacgccgcg ggggtgagca caggtttaat cgcgggtttg cacccagaac gcgtgaatca
     1861 aaccggggat atacccaagc agtgtcagca gaatattaat aatgaacgcc caaccgaacc
     1921 ctttaccgag cagcacgccg agcggcggca gaatgatggt gatgacgatt ctccagaaac
     1981 ccatatgtac tccctataag aaaattactc attgtttaaa aagagacttt atctcttaag
     2041 cgtagtaatt ttagcggagc ctgccagttt cagcttacgc ttttactctc atttacgctg
     2101 ctgaggctgg tatatgttgc atttttagtt tagagtttac ttaatttaga aaatacttaa
     2161 ataaatatga ctgaactcgc gcaattacag gccagtaccg aacaggcagc ggccttattg
     2221 aaagcaatga gccaccctaa acggttgctg attctgtgca tgcttagcgg ttcccccggc
     2281 accagcgcgg gagagctgac gcgcattacc ggactgagcg cctctgcgac atcacagcat
     2341 ctcgctcgta tgcgggacga agggcttatc aacagccaac gggatgccca acgcattcta
     2401 tattccatta aaaatgaggc ggtaaatgcc attatcgcca ccctgaaaaa tgtctattgt
     2461 ccgtaaggag tcactatggc tttgacaacc atttcgccgc atgatgcaca agaattaatc
     2521 gcacgcggcg caaagttaat cgatattcgt gacgctgatg aatatcttcg tgaacatatt
     2581 cctgaagcag atctggctcc attatccgtg ctggaacagt caggtcttcc acctaaatta
     2641 cgtcgcgagc aaattatatt ccactgccag gcaggtaaac gcaccagtaa taacgccgat
     2701 aaattagcag cgattgccgc ccccgcagaa atatttttac tcgaagatgg gattgatggc
     2761 tggaaaagag ctggattgcc agtagcggta aataaatctc aacccttgcc gttaatgcgc
     2821 caggtgcaga tcgctgcggg tggtttaata ttaatcggcg ttgtactggg ttataccgta
     2881 aatagcggtt tcttcttatt aagtggcttt gttggtgccg ggttactgtt tgcaggaatc
     2941 agcggttttt gtggaatggc aaggttgtta gataagatac cgtggaacca acgagcttga
     3001 gaagcgacgc cggacgcgcc ctagcagcga catccggcct cagtaattag atcaggaaat
     3061 cgtcgagaga tttaccttct gccagcgcct gagcaattgg cttcggtgta cggccctgac
     3121 cggtccaggt tttagtttca ccgttaacat cagtgaattt atatttcgcc ggacgcggct
     3181 ggcgtttttt accagcacgt ggtgcagcag cagagctatt acccaataac tcttccgggt
     3241 taattccgtc agctttcatc agctccagcc aggtgctaat tttttcctga cgctcagcca
     3301 gttcacgctg ctgctgttct tcttcttcac gcctttcttt agtgacaacc ctgaattttt
     3361 cgagcatttc ttcaagaacg tcaatggaga attcgcgagc catcgcacgg agggtgcgaa
     3421 tgttatttaa actttgtaac attacggaca taataaagaa aaccttttaa cgccaaaaca
     3481 aaaaagtatt tcgtcgcgta gtgtaagcga tcacttatta tatttccaca aaatttaatc
     3541 tatttacagt cctgcgcgag attatttcag ctgttatttt ctgaaactct cagaataatt
     3601 tcaagattat atattagcca ttttgatgag taatttaggt tattggtgaa cagagtggcg
     3661 ctctctgctc gctaactccg taagcctgac aaaaccgaag tgaataaaaa tgcaatataa
     3721 cagattaaaa gaaaacaccc ctcactggtg ctttctgctg ttaatgtaca ttttctggca
     3781 tcaaaaatgc tgtaaatgac ccggtcacga caaacaccga attctctacg cttatagcgt
     3841 tacctccccc ccaaactttc attagtgaaa ttaaaggcaa ggccatatcc aattgattta
     3901 attgaaaata aatcatatcg acatgtaaaa atccacaatg tacaaaaaac gagctgttac
     3961 ggaatatttt atctacaaaa actgactaaa taaaaatttt tcactaattg attagtaata
     4021 gccagcgata tacgctatgc gaaaatacag atggcaatga gatccactgc tttcatctcc
     4081 attaacatcc cattacgctt ttattaagga gcattagcat gttctcaccg cagtcacgct
     4141 tgcgtcatgc agttgcagat acgttcgcga tggttgttta ctgttctgtc gtgaacatgt
     4201 gtattgaagt tttcctctcc ggaatgagct tcgaacagtc tttttattcc agattggtag
     4261 cgattccggt gaacatctta attgcatggc catacggtat gtaccgcgat ctgtttatgc
     4321 gcgcggcacg caaaggctgg ataaaaaatc tggctgatat cctggcttat gtgacgttcc
     4381 agtcaccggt gtatgtggcg atcttgttag tggtgggcgc agactggcat cagattatgg
     4441 cggcggtcag ttcaaacatc gttgtttcga tgttgatggg ggcggtttat ggctacttcc
     4501 tcgattattg ccgccgactg tttaaagtca gccgttacca gcaggtaaaa gcctgactct
     4561 tcttttcgcg actggcatcg ccagtcgcgt ctttaactgg cttcgccaaa taaaccattc
     4621 aaataacgtt caagcgcaac acgcgaacta aagccatgcg ggataccgta atgctcgtgt
     4681 cgctgtcgct cgcctgctaa atagagcgga aattgctggt aatgatcgca agttttaata
     4741 tcggaagcgg gttcagccag tgttgaactg cgttctttta atgttggatg aattaccaga
     4801 gcggtacgtc ccattcgcgc ttcgcgatta acatagacat aattttcccc acggcggtaa
     4861 ccatacgctt tctgggttac cacatcgaca gtaaaaccga ctttttcaag tacgcgcgcc
     4921 acctcgtctg gtcgtaaata catatttgat cc
//
LOCUS       DAETPZ010000185         4858 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.187, whole genome shotgun sequence.
ACCESSION   DAETPZ010000185 DAETPZ010000000
VERSION     DAETPZ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.187"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            26..442
                     /locus_tag="K0W59_004074"
     CDS             26..442
                     /locus_tag="K0W59_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024856.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1094..1249
                     /gene="hokD"
                     /locus_tag="K0W59_004075"
     CDS             1094..1249
                     /gene="hokD"
                     /locus_tag="K0W59_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBI3024857.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1291..1476)
                     /locus_tag="K0W59_004076"
     CDS             complement(1291..1476)
                     /locus_tag="K0W59_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024858.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            1417..1695
                     /locus_tag="K0W59_004077"
     CDS             1417..1695
                     /locus_tag="K0W59_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024859.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            1697..2755
                     /locus_tag="K0W59_004078"
     CDS             1697..2755
                     /locus_tag="K0W59_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBI3024860.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHMKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTSTSILKQR
                     TKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            2756..3121
                     /locus_tag="K0W59_004079"
     CDS             2756..3121
                     /locus_tag="K0W59_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBI3024861.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            3118..3828
                     /locus_tag="K0W59_004080"
     CDS             3118..3828
                     /locus_tag="K0W59_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HBI3024862.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQVGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFV
                     MRTRSKKRRHFQSKVLQK"
     gene            4027..4102
                     /locus_tag="K0W59_004081"
     tRNA            4027..4102
                     /locus_tag="K0W59_004081"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4060..4062,aa:Ile,seq:cat)
     gene            4104..4180
                     /locus_tag="K0W59_004082"
     tRNA            4104..4180
                     /locus_tag="K0W59_004082"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4138..4140,aa:Lys,seq:ttt)
     gene            4183..4258
                     /locus_tag="K0W59_004083"
     tRNA            4183..4258
                     /locus_tag="K0W59_004083"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4216..4218,aa:Thr,seq:tgt)
     gene            4262..4336
                     /locus_tag="K0W59_004084"
     tRNA            4262..4336
                     /locus_tag="K0W59_004084"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4295..4297,aa:Gly,seq:tcc)
     gene            4629..4844
                     /locus_tag="K0W59_004085"
     CDS             4629..4844
                     /locus_tag="K0W59_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBI3024863.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT         1290 a         1079 c         1287 g         1202 t
ORIGIN      
        1 cagcattacc agggcggtaa tgaaaatgac tgaattaaca aaagaacaat taatcgaaga
       61 agccaaatta aaaatagcga ttacgaaatg ccaccccaat tcagggatgg cgcgagtaga
      121 gggcgagtta ttcaaaattg cacgggcatc gctggaagca gagccgatag cgtggcgata
      181 tcgctacgtg aaaaaaggtg ttatggactc tcagggggag ttgtgggttg gtgactggaa
      241 atatgtaccg aaaaaagagg attgtaacga caggccgaac tatgaaattc aggccttatt
      301 cactgcccca ccagtcccgg ttacatcaga agaactggtt aaagctgtgc acttttatga
      361 acaactaaaa cgcgaaaatc caccagcatc cggcaaccag attaatgggt taactatgtc
      421 ggttaaacga ccagccaact gaaaaagcgg aaacctgatt ataggttgcc agataaggca
      481 ataagctacc tggcgcggaa cggactgata agtatgggga atgttttacg atgagtattt
      541 agactaaaga gtttgtaacg ctatgtaagt gattttttct ggtttagata tttatatgtc
      601 cggccaaatt gaggtgtgtt taaatgttat tgcacattga ttgtaggggg aataatgaaa
      661 aacgcattgc agtttttgtt tgttgcgttc tggttgttcg catcatgtat gcccatcatc
      721 ttcacagcaa ggtatatgga aaaaattgat gttttgatat taatgtttgg acatataaat
      781 gccctttttt taggggtgtt catggcggtc atgtgcattg aatactggcg gtaaatacag
      841 cgaacgctat tggtttagtt ggatatttac tgtgccggac aaaaacggtt tgcagggaaa
      901 tcttagttaa gtagaatgac tgcgggtgct tgaggctatc tgtctcaggc atgaacacca
      961 aaaggcagat agagaaaagc cccagttaac attacgcgtc ctgcaagacg cttaacatta
     1021 atctgaggct caatccatgc tgaacacatg taggttagcc tcttacgtgc cgaaaggcaa
     1081 ggagaagcag gctatgaagc agcaaaaggc gatgttaatc gcccttatcg tcatctgttt
     1141 aaccgtcata gtgacggcac tggtaacgag gaaagacctc tgcgaggtac gaatccgaac
     1201 cggccagacg gaggtcgctg tcttcacagc ttacgaacct gaggagtaag agacccggcg
     1261 ggggagaaat ccctcgccac cgctgatgtg tcaggcatcc tcaacgcacc cgcacttaac
     1321 ccgcttcggc gggttttgtt ttttctggtc gttctggttt acaatccatc cgtcagcctg
     1381 aacaactggc acctgctgcg ccagcagaga aaacagatgg cgcacgatac caaattttac
     1441 aattcggata actctgccgc ccctgccagc aggcacgggc ggcgttctca tgcattcaaa
     1501 tctgactggt atcagcacga cccctgcacc gaagaacagg ctgaatggct gattcagcgc
     1561 taccgcagac acggatacga gattaagaaa gccctcagtc tcgattatcg tcactggata
     1621 atcttcgtca ggctccctta ttccgaacgg ccaccgcgtc cgtcctgcac attccagcaa
     1681 cggatctgga ggtaatgtgc gggtattact tcgacctgtt ctggttccgg aactcgggct
     1741 ggtggtcctt aagccaggcc gtgaatccat gccggtattc cacaataccc gggtactggt
     1801 ggagccggaa ccgaaaagca tgcgtaatct gccgtccggg gtcgttcctg ccgttcgcca
     1861 gccgctggtg gaagacaaaa cattgctgcc gtttttcagt aacgcacggg tgattcgtgc
     1921 tgctggtggt gctggcgcat tgtctgactg gctgttgcgc catatgaaat cctgccagtg
     1981 gccacacggc gattatcatc acagcgaaac cgtcattcac cgttatggta ccggcgcaat
     2041 ggtgttgtgc tggcactgcg acaaccagtt gcgtgaccag acatccgaat cactcgagca
     2101 acttgctcat caaaacctgt cagcatggat gattgacgtc atcggtcacg caataagcgg
     2161 tacgcaggag cgtgaattat ctctggctga attatcctgg tgggcggtcc gcaatcaggt
     2221 ggcggacgcg ctaccggaag cggtattacg tcgttcgctg gggttgcgtg cggaaaaaat
     2281 ccgctcaatg taccgtgaaa gcgacatcgt gccgggagag cagacctcca ccagcatact
     2341 gaagcagcgc acaaaaaatc ttgcgccgct gcctcacgcc caccagcaac agaatccacc
     2401 acaggaagag acggtggtca gcattgccgt tgatccggaa tctccggctc agtatctcca
     2461 gcgccagaaa ccacaacggg aagagatgcc tgtatacacg cgctgggtaa aaacgcagaa
     2521 atgcatgacg tgtggcaatc aggcagatga tccgcaccac atcattggtc atggactggg
     2581 agggatggga acaaaggctg acgatttgtt tgttattccg ctgtgtcgta aatgccataa
     2641 cgaactacac gccggggtaa aagattttga agaaaaacac ggtagtcagt tgttgttgct
     2701 gattcgtttt ttaatgcacg cgagaaattc gggtgttctg aagtggaaag cataaatgac
     2761 cgaacgcata gaatttgttt tgccttaccc gccaacggtg aacacctact ggcgacgtcg
     2821 tggcagcaca tattttgtat caaaagccgg tgagcgttat cgccgtgatg tggcgctaat
     2881 tgttcgccag cagcgactga aattaaacct gtccggaagg ctggcgataa agattattgc
     2941 agagccaccg gataagcgcc gtcgtgacct ggacaatatc ctgaaggcac cgctggatgc
     3001 gctgacgcat gctggactac ttatagacga cgagcagttt gatgaaatca atattgtgcg
     3061 cggtcagctc gttcctggtg ggcggctggg gataaaaatc acagaactgg ggtgcgcatg
     3121 aataaccagt atttacagtt tgtgcgtgag cagctcatta tcgccaccgc tgatttgagt
     3181 ggggcaacaa aaggtcagct tgaagcctgg caagagaatg ccatgttcga tacagggcgt
     3241 tacaggcgta aaaaaatccg gtaccgcgat gaagtgactg gaaaaatgat aacgcgggat
     3301 aatccaccaa tcccgggaaa gcaatcgctg gcgaagggga cgtcaattcc tctggtcagt
     3361 ccggttgagt tttcgacatc atcgtggcgg cgggctgttc tgtctcttga agaacatcat
     3421 aaagcctggt tgttgtggtg ttacagcggg agtatttgtt gggaatatca gatcgcgata
     3481 acacagtggg cgtggaatga atttaatgct caatccggta ccagaaaaat tgcagggaaa
     3541 acgcaggaac gcctgaaaaa attaatctgg ctggcggcgc aggcagtaaa agcagaactt
     3601 tttggtgggg aaggttatga ataccaggtt ggtggggaag gttatgaata tcaggagctg
     3661 gcattactgg cgggagtaac aaccaaaaac tggtccaaaa catttactgg tcactgggtt
     3721 gtaatgaaac acatttttca ccgactggat agtgaggctt tattgtttgt aatgagaaca
     3781 cgttcaaaaa aaagacggca ttttcaaagc aaagtgttgc aaaagtagat tgaaaggcat
     3841 atattcatgc aaatctgata ttttgccgat tttgtacgtg atggcaaaag caaacaaaac
     3901 ccgcccacaa gcgggttttt tgtgccactt atctcggata gacatggtga atgcgctggt
     3961 ggaggagata agggtgattt ttgaatgctt gcaacattga tttcgtaacg ttattatcct
     4021 gcgctcggcc ctttagctca gtggtgagag cgagcgactc ataatcgcca ggtcgctggt
     4081 tcaaatccag caagggccac caaccgccac tagctcatca ggaaagagcg tcaacccttt
     4141 aagttgagtg tgcgaggttc gagtccccgg tggcggtcca gtgccgactt ggctcagtag
     4201 gtagagcaac tgacttgtaa tcagtaggtc accagttcga ttccggtagt cggcaccata
     4261 tgcgggcatc gtataatggc tattacctca gccttccaag ctgatgatgc gggttcgatt
     4321 cccgctgccc gctccagtta gagtctttca gtctgcgatg atgggaaatc ccggagtgac
     4381 tgaaagacgt ttaagttatg aatgatcgct tttttttgca aaattgctgt gcagaaatac
     4441 taaccttcgg gcaggcgatc attcataagc actctgcttt tattccgatt aactgtgagt
     4501 ggtttgttgg atagagtgct ttccttactg tatatatcgt ttcgcccgct tttgcggttt
     4561 tttcttttca aatccctttc atttctcagt gtaaaactac gccatccgtt atttgcggag
     4621 gtgaggctat gaaatccatg gacaaaattt caacgggcat tgcctacggc acctccgcag
     4681 gcagtgctgg ctactggttt ttacagtggc ttgatcaggt cagtccgtca cagtgggctg
     4741 cgattggtgt actggggagt ctggttctgg gcttcctgac ttatctgaca aatctgtact
     4801 tcaaaatcag agaagacaag cgtaaggctg cgagaggtga ataatgtcgc catcatta
//
LOCUS       DAETPZ010000186         4825 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.190, whole genome shotgun sequence.
ACCESSION   DAETPZ010000186 DAETPZ010000000
VERSION     DAETPZ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.190"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            446..1201
                     /locus_tag="K0W59_004086"
     CDS             446..1201
                     /locus_tag="K0W59_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBI3024864.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            1280..1353
                     /locus_tag="K0W59_004087"
     tRNA            1280..1353
                     /locus_tag="K0W59_004087"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1312..1314,aa:Gly,seq:ccc)
     gene            1488..3095
                     /locus_tag="K0W59_004088"
     CDS             1488..3095
                     /locus_tag="K0W59_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBI3024865.1"
                     /translation="MLNSRTYLYQRNGVFYIRLRMKTTGRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATEHLTVDQHRYINDVIGVLKACQDRLKGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPDPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVSKATRADMLRVREVLQLLPKNCKQRFKAMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTVLAAITGARLNEVAQLQVKDVRTTEAGTVYIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTMGDNAMVFDGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVDRLVNVMAGVYKETGVNG"
     gene            3088..3741
                     /locus_tag="K0W59_004089"
     CDS             3088..3741
                     /locus_tag="K0W59_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HBI3024866.1"
                     /translation="MAEYYPAEFGGTAFNCPNCGAYSQQRWVRLKYGANGIYETPFAF
                     SKCERCNEKAYWYDETMIIPASANVEMPNPDMPDDCKSDYMEARSIVNLSPKGAAALL
                     RLSLQKLMVHLGEPGKHIDTDIKSLVAKGLSPLVQRAADICRIVGNQAVHPGEINIDD
                     DPQLAHGLFKLLNIIVTEQITRPKEVEAMFNSMPERALKGIEDRDRKAREQQQAASE"
     gene            4682..>4825
                     /locus_tag="K0W59_004090"
     CDS             4682..>4825
                     /locus_tag="K0W59_004090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024867.1"
                     /translation="MSTQPLMKHEVVLPVIVDGYHWACNQDRMWNLNELHKELELPSA
                     KEPG"
BASE COUNT         1278 a         1149 c         1212 g         1186 t
ORIGIN      
        1 cacccgtagc ggttgcttcc cgcgcttcca tagaaatccc ctcgatttga acatttggtt
       61 cttatggcaa agatttagtc taagcaggca agatttacgc cagaaacgac gataagcgtt
      121 aggggaggaa ggaagtgtga tgacgttaaa aaaacacaga tatgatacga aacaggactc
      181 tgaataggga cagtgatacg tatcaatatg agtggcgata aaatcaatgt gataaatcag
      241 gattattacg catcaggatg ggaaataggg taaaaagtca ggaacttcga tacctctaca
      301 gcgaatattt tgctgatgat acttcccttt gccaaatgaa attaacctct ttctctatta
      361 ccacgttttt ccagaagcaa gagattgggt atcaagaggc tggctgctat gatatggcgt
      421 cttgtttttt aagcgaggaa agattttgag tgcgggacgc ctgaataaaa aatctctggg
      481 tatcgtgatg tttttatcgg ttggactgct attggcgggc tgttcgggta gcaaatcatc
      541 cgatacagga acgtattccg gctccgttta caccgtgaaa cggggggata cgctatatcg
      601 tatttcgcgc accacgggaa ccagcgtaaa agagctggcg cgactgaacg gcatttcccc
      661 cccttacacc attgaagttg gtcagaaact aaaactgggt ggggcgaaaa gtagcagtag
      721 tacacgtaaa tcaaccgcca aatcaacgac caaaaccgca tcggttacac cgtcatcagc
      781 ggtaccgaaa tcttcctggc cgccagtagg gcaacgttgt tggttatggc caacgacagg
      841 gaaagttatc atgccgtatt cgacagcaga tggcggcaat aaagggattg atatctcagc
      901 tccacggggt acacctattt acgccgcggg tgcaggaaag gtggtgtatg tgggcaacca
      961 gctgcgtggc tacggtaatc tcatcatgat taaacacagt gaagattaca ttacggctta
     1021 cgcccataat gacacgatgc tggtaaataa tgggcaaagc gtgaaggctg ggcaaaaaat
     1081 cgccactatg gggagcacgg atgcggcatc tgttcgcctg catttccaga ttcgttaccg
     1141 tgcaacggca attgatccgc tacgttactt gccgccgcag ggcagcaagc caaaatgctg
     1201 atggcgaatt aatcagcagt cagcagcgcg gcacttgcta aggagagcgt aaggtttata
     1261 atgccttacg catctcgaag cgggcgtagt tcaatggtag aacgagagct tcccaagctc
     1321 tatacgaggg ttcgattccc ttcgcccgct ccatgacatt tcttaaagtt caataactta
     1381 ctgaatccta agagatatgg cttaacgcct tccgatagtt ttactcactg taaccaacac
     1441 tgtgactaac tttgttgaag tagtgagcag acctaaggtc tcagtacatg ctcaactcaa
     1501 gaacctacct ttatcagcgt aatggtgtct tttacatccg tcttaggatg aagaccactg
     1561 gtcgccttac cgcttcgctt ccttctcaca accgttataa actagcgtca gtatctttac
     1621 ggaccaagga tagacgtacc gctatggctc actcccggca catcaagtca gcacttaaag
     1681 caatccatgc agacaaccct aacgcctctt atgaggagct acgggagcac ctgaagacca
     1741 ttgtcgagtg ggaactcagt gtcagccgcg atgacctgaa cgaccctgag tcctatcagc
     1801 tttacgttga ccagtacgac gatattaagt ccaaccttcg ggaagctgta gcgactgagc
     1861 acctgactgt agaccagcat cgctatatca atgacgttat cggtgtgctt aaagcctgtc
     1921 aggatagact caaaggtgat agctctggtc tgttgtctta ccttgagcct gagactggca
     1981 gtctaagacc gtccgtctcc ctatctgtat tggcagaacc tgaagtccct gaccctaaag
     2041 ctctgaccct cgcgtctctc attgagcagt acgagcagga gaacgcccag aactggaagc
     2101 cagcgaccct gagcgagaac cgagcgtcgc actccacgct aatcgagata ttcgactatc
     2161 tggatattca ggacgtcagc aaggcgaccc gtgcagatat gcttagggtc cgtgaagtcc
     2221 tccaactgtt accgaagaac tgcaaacaac gctttaaggc tatgccgctg tctgacctgc
     2281 tgaaccggga gtctaagact gactgtctgg atgtcgttac catcaacaat aaatacctta
     2341 tcaagatggc tgctgtgttt aagtgggcgg tacgcaacga cctgatagct aagaacctga
     2401 cagaaggtct tgagctgaaa gtcccgcagc gtaaagcctc cgacgctcgt gatgcgttca
     2461 gtcctgagca ggtagggcaa ctactggtcg cagctaaagc gtactcgcag aagacctcag
     2521 ggaagccata ccattattac gtcaccgttc tggctgcaat cactggagcg agacttaacg
     2581 aggtggccca acttcaggtc aaagacgtca ggaccactga ggccgggacc gtgtatatcc
     2641 atatcaacga agacgatagc agcttgccgg gtaagagcat caagaacgcc cacagtgacc
     2701 gctgtgtccc gctggtcgat ggcgcttatg gttttgtatt ggctgacttt atgtcgcttg
     2761 tggaagaccg tagaaagact atgggtgata atgctatggt attcgatggc ctcaagctga
     2821 tgaagaacgg ctatggtgag caggtgagca aatggtttaa ccgaacgttg ctacctaaag
     2881 tccttgctga ccgtagcggc ttagcgtttc actcgtttag acatacagtt gcgacccagc
     2941 taaaacaaca tggggtcgaa ttggcgtatg ctcaggcgat tatgggtcat agctctgggt
     3001 ctataactta cgaccgttac gcgaaagagg tcgaggtaga tagattggtt aatgtgatgg
     3061 cgggtgttta taaggagact ggagtaaatg gctgagtatt atccggcaga gtttggtggc
     3121 actgctttta actgcccgaa ttgtggcgct tattctcaac agcgctgggt ccgcttaaaa
     3181 tatggcgcta acgggattta tgagacacct ttcgcctttt ctaaatgtga acgatgcaat
     3241 gagaaagctt attggtatga cgaaaccatg attataccag cgtcagcaaa cgttgagatg
     3301 cctaacccag acatgccaga cgactgtaag tccgactaca tggaggccag aagcattgtt
     3361 aacctttctc ctaaaggcgc tgccgcactc ctgcgcttaa gtcttcagaa gctgatggtc
     3421 catctcggtg agccggggaa gcatatcgac acggacatta agtcgcttgt agccaaagga
     3481 ttgtctccat tggtccagcg agctgctgat atttgtcgta tcgttggcaa tcaggcggtc
     3541 catccgggtg aaattaatat tgatgacgac cctcagttag cgcatggtct ttttaagctg
     3601 ctgaatatta ttgtcactga gcagattacc agaccgaaag aagtcgaagc catgtttaac
     3661 agcatgccgg aaagggcgct caagggcatt gaggaccggg acaggaaggc gagggaacag
     3721 cagcaggcag ctagcgagta aagctttagg tcagattgaa tataccctca ctatagggaa
     3781 gggggcttaa ctatcactat aggggagaga ccctaagtcc ccaccttaat agcagtcacg
     3841 ccccatacgc tcattcattt tagtctcagg cttccggtag cggaccgatt ggaaaccatc
     3901 cgcagacgcc tagtgttaaa tcaccttggc ctgagaccat tctcctttat cgtagcaatc
     3961 tctctccttg acaggcggag aacatcagac cgcagcggaa gtatccgtct atgcgaccat
     4021 cggtgtgcga gattgtgaag aaccaagaca tcattgctga ggtctgccat ccattgtgga
     4081 caggcggaaa ctcaaagagg ttagaccagt agtggtgtct gaggtttggg ctttgagtcc
     4141 tgaccagcag gctggaggag accatgagtt tatcttgtta tggattgtgt tatcacaatt
     4201 ccaataagaa gaactttaag tccagcctcc agtcctgtca gcccgaagta aactatggtc
     4261 gctatgatgt ggtctccata cttcgggtct aactggaggt cagattatgg ccttatcagg
     4321 tagcaaatct tacaccctca ctgtagagac catacattcc gcctctggtc ttgatgcgtc
     4381 aagttatgcg gaagctctcc cgacattaag tctttctccg aagacatccc aaggcaagac
     4441 agcctctgtc agacctccag cccctagaag ttcttctcct cttgaagtct atcagcttct
     4501 agcatctggt cgtcctgaca cgctgtcgaa ccttcatatc tatggtcatc gcagaccaca
     4561 tcacaattac ctcaagtctt tctgtatgtc tcctgttgcc tgtgagtctc cctatcgttg
     4621 ggggcttgag taggctctca gggtgtgact tcaggaggca taacaaaagg agtacaacga
     4681 tatgtcaact caacccttaa tgaagcatga agtagttctt cctgtcatcg ttgacggcta
     4741 tcactgggcc tgtaatcagg accgtatgtg gaacctgaac gagctacata aagagcttga
     4801 gcttccatct gctaaggagc cgggt
//
LOCUS       DAETPZ010000187         4735 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.192, whole genome shotgun sequence.
ACCESSION   DAETPZ010000187 DAETPZ010000000
VERSION     DAETPZ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.192"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..181
                     /locus_tag="K0W59_004091"
     CDS             <1..181
                     /locus_tag="K0W59_004091"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HBI3024868.1"
                     /translation="VSDKPLHGEIKLPGQANRFYEGAISEHLQIGIRAIDLLRAEGDR
                     LHSRKLRTFNEPPFR"
     gene            524..1240
                     /locus_tag="K0W59_004092"
     CDS             524..1240
                     /locus_tag="K0W59_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI3024869.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(1696..1771)
                     /locus_tag="K0W59_004093"
     tRNA            complement(1696..1771)
                     /locus_tag="K0W59_004093"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1736..1738),aa:Asn,seq:gtt)
     gene            complement(1872..3499)
                     /gene="yeeO"
                     /locus_tag="K0W59_004094"
                     /pseudo
     CDS             complement(1872..3499)
                     /gene="yeeO"
                     /locus_tag="K0W59_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            3504..3579
                     /locus_tag="K0W59_004095"
     tRNA            3504..3579
                     /locus_tag="K0W59_004095"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3537..3539,aa:Asn,seq:gtt)
     gene            complement(3617..4567)
                     /gene="cbl"
                     /locus_tag="K0W59_004096"
     CDS             complement(3617..4567)
                     /gene="cbl"
                     /locus_tag="K0W59_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HBI3024870.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT         1438 a         1110 c          970 g         1217 t
ORIGIN      
        1 tgtttcagat aaaccgttac atggtgagat taagcttccc ggccaggcta accgttttta
       61 tgaaggcgct atttccgaac acctgcaaat tggcattcgg gcgatcgatt tgctgcgcgc
      121 agaaggcgac cgactgcatt cacgtaaatt acgaaccttt aatgagccgc cgttccgata
      181 ataagttatg aaccttctta tcagaaatag atgtgaagga ttaagtaaga catctggcaa
      241 atagcctgca ataaacgcgg gctgcttgct tccttgggca gatgcgagtt ttattatcgt
      301 cttaatgatt tccacatatt aaaagcaagt atgctttcaa aacacaatta taaaaaatcc
      361 cgccaacaac attagttttt ataaaattaa atataagatt atggctttag aatattttta
      421 tttctaatag acgagatttt tcctgttatg atataatatg ccgaattaac acatgttaac
      481 gatttaccag taatgtaaat aaattttcga ggagatcatt ccagtgggac gtaaatgggc
      541 caatattgtt gctaaaaaaa cggctaaaga cggtgcaacg tctaaaattt atgcaaaatt
      601 cggtgtagaa atctatgctg ctgccaaaca aggtgaaccc gatccagaat taaacacatc
      661 tttaaaattc gttattgaac gtgcaaagca ggcacaagtt ccaaagcacg ttattgataa
      721 agcaattgat aaagccaaag gcggcggaga tgaaacgttc gtacagggac gttatgaagg
      781 ctttggtcct aatggctcaa tgattattgc tgagacattg acttcaaatg ttaaccgtac
      841 gattgctaac gttcgcacaa ttttcaataa aaaaggcggc aatatcggag cggcaggttc
      901 tgtcagctat atgtttgaca atacgggtgt gattgtattt aaaggaacag accctgacca
      961 tatttttgaa attttgcttg aagctgaagt tgatgttcgt gatgtaactg aagaagaagg
     1021 taatattgtt atttatactg aacctgctga ccttcataaa ggaatcgcgg cgctaaaagc
     1081 agctggaatc actgagttct caacaacaga attagaaatg attgctcaat ctgaagttga
     1141 gctttcccca gaagatttag aaatctttga agggcttgtt gatgcccttg aagatgacga
     1201 cgatgtacaa aaagtttatc ataacgtcgc aaatctctaa ttatatttta aagaaatctg
     1261 tctttatggc agatttcttt aatctcatat aattcttata aaaaatataa tgtttaactc
     1321 gtcatattgc ttataaaaaa tccccgttcc cagagaaata atatttatta aaatggcagg
     1381 tcttcttttt ctgattaccg aagacaaagt ggcaattacg tatagtttcc cgataaagac
     1441 gcgacagcta catcccgcat aaggcatttt tctctttatc tttgtacggt acttcatgga
     1501 acagaatttt tgaccttgtg aatcgtgatg tctgttgggg aggggcaatt tgctcactga
     1561 agcgtgagac tcgattaagc gcacgaaaca cagaaatcaa aaaacccggc cactttttta
     1621 caaagtggcc gggtaaaaat aatttttatt ttttaactgt cctgagtctc atagagatgt
     1681 ctcaaaacta aaatttggct cctctgactg gactcgaacc agtgacatac ggattaacag
     1741 tccgccgttc taccgactga actacagagg aatcgtgaga acgaggcgaa tattagcgat
     1801 gcccacccac aatgtcaaag cctgtttttt aaatttgaaa tcgtttgctg aaataatctg
     1861 cattttgtcg tttattccga cacaactggc tttttttcac acttttgcgg ctcggatcga
     1921 gggtatttcc acagccaacg tccagtaacc attcgccagt aaaacagcac ggcccgcaca
     1981 gcccagtagg caaacattcc catccagaca ccaaccacac cccagccaag catgattccc
     2041 tgcacataac cgactacaac ccggcaaccc cacatgctca acatagaaac ccacatggcg
     2101 taacgggcat cacgagcacc cttaaatcca gcgggtagca cccatgaggc ggaccaaata
     2161 ggcataaata aagcatttag ctaaatcaga atcacaacga cgtgtttaac ctgtggatcc
     2221 tgggtgtaaa acgatgccat aaccccggca aagggagccg ttagccaggc gatggccgtt
     2281 aatccaagag tggacagcca gaacacatgc cgcaactgaa tctctgcttg cgctatctgc
     2341 cctaccccca accttcggcc tgtaatgatc gtagaagcag aaccgagcgc acttccgggt
     2401 aagttgataa gagccgcaat tgagaacgcg ataaaatttc cggcaataac gctggtcccc
     2461 atcccggcaa cgaacatttg ggttaataac cgaccactgg taaataacac tgattcgaca
     2521 ctcgctggaa taccaatccc catgacttcc cagataatgc taaaattcag cggtttaaaa
     2581 tagctcttta acgaaatcct tagcgcagga ttaaaaccaa tcgccagcac ccacaaaatt
     2641 gcaactgcgc caatataacg agaaatggtt aaacccaacc cagccccgac caggagaaaa
     2701 ggccgtaaat caatatgccg ctaataataa tattaagaat attcaggcta ccgttaatca
     2761 gtagcggtat tttcgtattc cctgcaccac gaagtgcccc gctaccaata agagtgatgg
     2821 cagcggctgg ataactgagt acggtcagct ccagataagt caacgccagt gctttaactt
     2881 ctgtcgtggc atcacccgcg acgaaatcaa taatttgttc gccaaaatga tgaattagcg
     2941 ttgccaacag tacggcaaac aacgtcatga tcaccaatga ctgccgcgtc gccaccctcg
     3001 ctcgtcgtcg atcccgctta ccgagactaa atgctacaac gacagtagta ccaagatcga
     3061 tagctgcaaa aaaagccata atgaccatat tgaagctgtc cgccaacccc acgccggcca
     3121 tcgcatcttt tcccagccag ctgaccagaa aagtgctcag gacccccatc aacaggacac
     3181 aggcgttctc catgaagata ggaacagcaa gcggggttat ctcgcgccag aacaacactt
     3241 tgtagctctt gcgtttagag tgccagcgag tgccgtgaac aacctggcgt aaagcagagg
     3301 agatattcaa agccgacctt aattgcagaa agtgaaacca catttcaaat gatgagggag
     3361 aatcagcaaa gctgcaaaga ttttcgccaa caaattgtct gcaaatgcaa caaactgttg
     3421 atagaaacgg caaacagttg ggaaatttaa aaatcgggtt tgacaaaata tttttcgccg
     3481 ttaagatgtg cctcaacagc gattcctctg tagttcagtc ggtagaacgg cggactgtta
     3541 atccgtatgt cactggttcg agtccagtca gaggagccaa atttctgaaa agcccgcttt
     3601 tatagcgggc tttttgctat atctgataat caatttcctc ttcactgttt tccatcacct
     3661 ggcgcttgat atcctcaact gacagtcctg cattacaaag ttccagaaag cgccaaacat
     3721 agttacgctg aagttgtcct cgcttcagtc ccaaccagac agtattagca tcgaaaagat
     3781 gccgcgtatc caggcggatt aaattctctt cctcttgttc gccgctggat tgttcggcaa
     3841 ctaatccgat cccaagccca agagcaacat aggttttaat gacatcagaa tcctgtgcac
     3901 ttaatacaat atctgccggc aaacctttgc gggtaaatgc gtcatcagtg cgtgagcgcc
     3961 ccgtaattcc ctgtcggtaa gtaattaacg gccacttcgc tattgattcc agcgtcaatg
     4021 gtgtaatttg cgttaaggga tgatcgagtg gaacaagcaa actatggtgc caacaaaacc
     4081 acgggaaggc gacgagctgc gggtcattac tcaaacgctc gctggcgata ccaatatcag
     4141 cttcgccatt ttgcaacaac gtcgcaattt cctgtggcga accctggatt agctcgagcc
     4201 gaacctccgg gaaaagttcg cgaaaagctt taatgacctc tggcaagcta taacgtgcct
     4261 gagtatgcgt cgttgcaata gtgagaacac cagacgtatc gttggtaaac aggtctgcaa
     4321 gccgacgaac attactggct tcattcagaa tacgttctgc aatgaccagt aatgctttgc
     4381 ccggttcagt catccccagc agtcgcttac ctcgtcgaac aaatatttcg atgccaagtt
     4441 catcctccag ttcccgaata tgacggctaa cgcctgactg tgaggtaaaa agcatattcg
     4501 caacctctgt caggttgtaa tcctgacgtg cagcctcgcg gattatcttt agttgttgga
     4561 aattcacggt aaactccggg cagttcagat atcccgttat tgttaaagtc taatgcccgg
     4621 cataacaaat aataaaaacc cgcatcttat tccatcccga tataacgctt agctcaccaa
     4681 ttgccactgc cttttttcca tcactggaga actaatcact gacattaaca actct
//
LOCUS       DAETPZ010000188         4714 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.193, whole genome shotgun sequence.
ACCESSION   DAETPZ010000188 DAETPZ010000000
VERSION     DAETPZ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.193"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(35..145)
                     /locus_tag="K0W59_004097"
                     /pseudo
     CDS             complement(35..145)
                     /locus_tag="K0W59_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(469..1146)
                     /gene="ospD1"
                     /locus_tag="K0W59_004098"
     CDS             complement(469..1146)
                     /gene="ospD1"
                     /locus_tag="K0W59_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HBI3024871.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNSHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(1517..1654)
                     /locus_tag="K0W59_004099"
                     /pseudo
     CDS             complement(1517..1654)
                     /locus_tag="K0W59_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1736..2034)
                     /locus_tag="K0W59_004100"
                     /pseudo
     CDS             complement(1736..2034)
                     /locus_tag="K0W59_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2623..2782)
                     /locus_tag="K0W59_004101"
                     /pseudo
     CDS             complement(2623..2782)
                     /locus_tag="K0W59_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2786..4015)
                     /locus_tag="K0W59_004102"
                     /pseudo
     CDS             complement(2786..4015)
                     /locus_tag="K0W59_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(4035..4382)
                     /gene="tnpB"
                     /locus_tag="K0W59_004103"
     CDS             complement(4035..4382)
                     /gene="tnpB"
                     /locus_tag="K0W59_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBI3024872.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(4382..>4714)
                     /locus_tag="K0W59_004104"
     CDS             complement(4382..>4714)
                     /locus_tag="K0W59_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3024873.1"
                     /translation="RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQY
                     RKGGLLPSGKNMPALLPVTLTPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQILI
                     REIKGSSH"
BASE COUNT         1168 a         1115 c         1034 g         1397 t
ORIGIN      
        1 agtatgatcc tacccacgta atatgaacac aggcttaagt gaggttctgg ttttcaaatt
       61 gttcaggact gagacctcca caccaactgt gccgtcgtca gcgattgtaa tcgcacttga
      121 tataattaaa cactgtatat ttcatttgtg gagtctccag tctgaggggg ctttatgcat
      181 tccgtattgg gcactatgat gccagaaatc tgcaaggctc cacaccttcc tgtatttccc
      241 ttcctctatc acagtgcact ttacttaata gggtgggatg tgttcattac atttattgtg
      301 ttttttatga ttgtttttgc gtccagtgtc gttcgtcatg ggaaactggt tctatgggta
      361 gtatcgctca gtctagttaa ctgtttgttt ctttttagaa attaagcaga tcacacaatt
      421 cggggagaga tccttctaag tgcaattctt tgcagctatt tagcacactt aaaacaactc
      481 ttgtattttg tcagaaataa tattaaacat gtcttgttca ttattgttaa gtctgtctgc
      541 tatttctgta agttttatcc cactatgatg gcatatattt atatatgcca tcatagcctc
      601 gatattccct ttccctgcgg ccagaaataa tcctggtaac ccctcattat ctcttacatc
      661 caatagttta atcgtttctt ttggtgataa atcaaaagtt ttaataaaat ctccataagc
      721 ttgaatcgca gcggaatgac cttcttgaag agctatgaac aaagcagatt ccccagcatt
      781 atttcgtgct gcaagaactt ctagtttttc ctctttgggt atttcggctg ccaacagcga
      841 ttcaccgtat ttacgcgctg catcggcatt tccatgccaa atcaatgaaa acaaattatg
      901 gctgttatac tccttattat tctttgtcgg aatggatatg ctctgtattt tggccaattt
      961 tctgaaagaa acaccactat atcccccccc tgatttttct tcattgatgg cgtgggatat
     1021 agcggtattt gtcacgttaa tgatattatt tttcattcct ttattttcat tagcagtatt
     1081 gctgcctatt attaggtgca tatttttgtt gtttgctgga tgtaatccat agttatttat
     1141 tgacattttt gttcatcctt tttgctaaaa aaaacaataa cctatttttg taatgtaata
     1201 gatattagtc tattgctaat ctaaaaaaac cattgccaca ttgaatggct atattcacca
     1261 ataaattctt tcgatttcgt atattgacat atcgtagata tatcttatcc ctaagattca
     1321 ataatcgagt ccgtttacca ttctgtgcaa gcgccttcac tcagaagtga ccgtcaaggc
     1381 gatcaccaaa cttgataata aagcagctca ttgccatacg ccagtcctta aggggcgttg
     1441 tccatttctg tgatgctgcc tagatttaac tgccagctcc ggcttatcta catctaaata
     1501 tgtgagccta ataccgctac actttgtcca gactatgttt ttctccggga agtgggctaa
     1561 ccgtctccat aaagtgacga acttttttca acagtggcca tattgaacgg cactgatgat
     1621 tacgggttat tgtctcatga agagccggcc acatcactga cagcagtgac ttcaggcctt
     1681 cagcaccaga cagcctgaac cagttaatcc atcgcccgac agatttagta atctagggct
     1741 ggtttttctt cagctgttgc ggttcgaaag tgccattacg atcacgcaga gtacggattt
     1801 ccagaggttc atctccagta gtgataatct tcgttaagca accattgcgg gaattgctgc
     1861 cagatctaag ctgatttttc tcgcacccaa gatgatgagt catattagca ttgagtacgg
     1921 tttcattttc agcatccaat caaactgact gagatcttca ggatttttga gacttttggc
     1981 tagttcatta gctaaagccc gcaacttatt agatgtcaac tgtttttcat tcataaattc
     2041 accttcattt gatatcaaat taaatagttc aacatcagag aattatacaa acttatgtac
     2101 aggctcctat gatcttcgaa tcctgaagat tatagtgaaa ataagttttc acgttgaaat
     2161 cccacatagt tagtatatga taaataatca tggatgacct gattaacctc ttggggtagt
     2221 gcttttatag aagaataaat attctactaa tttaatacat cacctaaaat agtattgttt
     2281 tttatggttg gttgaaggtc gtggtgatga tgttagtcct tttgagaata ttccagtgcc
     2341 agatcttact tataataaag gggcttggca gtttggataa actattgttc ttattccact
     2401 aatacaaact ttttgtaaag tatattaaat atacttcatt tcatcctgaa atccccggaa
     2461 gtattcaatc aaacaattac accataaaag gaagaggggg ccctaattaa gttgtcaagc
     2521 atgtttatga tacctgcagg gtataaattt tctggctgca agcagacaca tataaaacgt
     2581 ctttattgat cctacccaca taatatggac acagtcgtaa gtgaggctct ggttttcaaa
     2641 ttgttccgga ctgagaccac cacaccaacg gtgccgtcgc cagcgattgt aatcgcactc
     2701 gatataacta aacactgttg cccgcactat ttcccgatgg ataatagtgt tctccgtgga
     2761 gacattccat tttcagcgaa tgaattttca tctgttgctg tatccagtca tacagtgact
     2821 gcatcagtgg cgcggctctg gcttttcttg ccgccagacg ctgttctgct gaacatcccc
     2881 ggacttctgc ctcgatggca tacagttcac cgatacgctg cagggcttcc gtggtgatgt
     2941 cggttggcac tcttgcatgc acatcgtgga tttttctccg gacatgagcc atacatgcgg
     3001 cttccgttat tctgccggat tcgtacaacg cccggtaacc accgtaagca tcggcctgaa
     3061 gcactccgct gtaaccggcc agatgattct gtggatggat acctttccgg tccggtgagt
     3121 acgcgaacca gaccgccgga ggcatttccg aaccggcgtt acggtcatca cgaacgtaga
     3181 cccacagccg ggcagtcctt gttttaccgc tgcccggctc ccggaccgga acggggatat
     3241 catcggcatg gactttaccg ggcatcagca catactggcg caggatgtca tacagcggtt
     3301 ccagcagttc agcaacagca cctgtccagc gccccagtgt ggcacggctc agctccacgc
     3361 cctgacgacg gtatatttct gactggcggt ataacggcag atagtctgca tattttccag
     3421 cgacaacatg ggccagaagc cccgctccgg cataactgcg tgcaatgggt tttgaaggca
     3481 ccggtgcctg cacgatatgg tcacaccggc aacaggccag tttcggacgt tgtgtttcga
     3541 taaccttaaa ggcgctgctg ataagctcca gctgctctga cacatcacat cccagagaac
     3601 tgagttcgcc accacaggcc gggcagcatt cctcttccgg acggatgacc cgggtttcac
     3661 ggggaagtga ggcaggtaac ggtttacggg ctgaagactg gcgcagggga gatggcagta
     3721 ccgggtcata ttgctcaccc agcgtttccg ccatttcttc ctgaagtgcg ctgattcgct
     3781 cctgtgcttc cagtatctgc cgttcggttt ttgcgcgaag tttttctgag cttttaccga
     3841 actgcatgcg ctgcagtttc gcaaccagcg ccttcagccg gttgatttcg gaagcataag
     3901 ccgccacccg ctgtgagagc aggcggttgt atttcgccat ctggcggatg gtgtcctgtt
     3961 gcgtctgcaa cagtgcccgc aggcgggcat tctcatgaga aagtgaggtg tccatatcct
     4021 cactttacaa cgggttatat gcggatgcca gcgcgttccg ttcgtttcgg gtgtttccag
     4081 tcgatgccct caagaagcat ggataactgg gccggagtaa ggtgcacctt gccgtcacgg
     4141 gtgaccggcc agacgaagcg gccccgctcc aggcgtctgg taaagaggca cagtccgtca
     4201 ctgtcagccc acaacacttt tatctggtca ccccggcgtc cgcggaagat gaacagatgc
     4261 ccggagaacg ggtcatcctt caggacgttc tgaacttttg aggccaggcc gttaaagcca
     4321 tttcgcatat cggtgatacc tgcaaccagc cagatacgcg aacctgcagg gaaagatatc
     4381 atcagtggct gctccctttt atttcgcgga taagtatctg taataacgcc ggcgtcagtt
     4441 tacctttaag cctgagagtt ccggccggca gaaccagctc acagcacaga ctgtcggacg
     4501 gtgtatttat ctgctccggc gttaacgtca cgggaagcag tgcaggcata ttttttccgg
     4561 aaggcagcag gccacctttc cggtattgat ggcgccagtt gaagagcagg ttatcgttga
     4621 ttccgttttc acgggcgatc tgcgccacac aggctccggg ctgcagtgac tgctccacta
     4681 aagcgatttt aaattcataa ggaaaattag gccg
//
LOCUS       DAETPZ010000189         4674 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.194, whole genome shotgun sequence.
ACCESSION   DAETPZ010000189 DAETPZ010000000
VERSION     DAETPZ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.194"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(695..1744)
                     /locus_tag="K0W59_004105"
     CDS             complement(695..1744)
                     /locus_tag="K0W59_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBI3024874.1"
                     /translation="MYNNKIDNNRKTRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSIFDYIKSKFYRLMIPYFTFAFTILIINTINSGETI
                     DYIYSHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFKEIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            2590..2787
                     /locus_tag="K0W59_004106"
     CDS             2590..2787
                     /locus_tag="K0W59_004106"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024875.1"
                     /translation="MLGDNPNIDVVAMVPIIIVDMKIEVTLKLSDITVGNIMSAVSIM
                     AKDTLNTESVEKRLLSEKCMA"
     gene            complement(3728..4645)
                     /locus_tag="K0W59_004107"
     CDS             complement(3728..4645)
                     /locus_tag="K0W59_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBI3024876.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIIFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKDKK"
BASE COUNT         1552 a          830 c          816 g         1476 t
ORIGIN      
        1 ctacaatgat ttcaacttat tttatttaat tgatattacg aaacttaact catacataaa
       61 caatatgact caatactgtc ctaaatagct gcgcagaaaa gtgctccact ttcgctcagc
      121 ccttgctggg cgaagcatca ggaaggcgtt ctcagctagg ttgtgttata tctggcttga
      181 gttttgttgc ccatacgcaa cacattaccc atctgtaaac gacaagtgtg acagaattag
      241 gtgcagctat ttagactata tcgacgcagt aacgataata gatatcgaaa ctgtactaga
      301 tattagctac ttatggttac cggggggagt cttttgagca ctggtgttat caatcggctt
      361 caaataattc accaataaat tatattgcga tagctcatca ccttccaggt tgtttatagc
      421 cagtttatca gcgtcaccac ctactgttta tgtttttatt tctttgatta ataagtatat
      481 gatattttaa ttcatctggt gtatttcttt ctcatgtgtt ggccgactga atatctgttc
      541 agtgaagggg taattttatg tttgcacttc gaacattccc ttcagttgct aggaaagaaa
      601 accgtaacat ttgcgcatta attttaacag tataatctga gcataaatat gcgctatata
      661 tctataaaaa ggcgcaatta attgcgcctt tttatcattt tatcaaaaat ggcccaccga
      721 ctgtcctcga tagccaatgg ccaattttat ctgatacata tacacaaata caggtatata
      781 tgattgcgca gataggattt ggaatggtca cgctgaagcc tatgattgct aaagccatag
      841 ggataaataa tttggtgata tactccattc cacatatgtt aagagtattt cttccagctc
      901 ttacaataaa gtcattccca caaattattt ttgctataaa aatattaaat attatcaaac
      961 ctaccgtggt gataatagta ttaatagctt gaatataact acttgatatt atgtctgcat
     1021 tgaatattga gataatctta aaaagcaaag gtttctggtt aaggagttga tacgcactca
     1081 ttatcgcggt aattgagaag acaatgaaac caattttaga ttttccaaaa aacctgtcac
     1141 gtgttaattc taaaaacata catctaccga gaggcaataa ccaccagtat gccatagcgg
     1201 agtctatgtt ccaaaaccat tgaggatcta gtaatgggtt atgattgagc actgtttgtg
     1261 acaacatgaa cgataatagt gatatgatta aaatcactat attgttcttg actatttctt
     1321 taaagattgc atcaattgct attataacaa aaaggcagtt tataaaccat attgttccta
     1381 caaattggtt gtttctaaca ccatatatta tatcataaat gtggctatat atataatcaa
     1441 tcgtctcacc tgagttaatt gtgtttatta ttaatatagt aaatgcaaaa gtgaaatatg
     1501 gaatcattaa tctgtaaaac tttgacttga tataatcgaa aatagataag tcattttttt
     1561 tgatagtaaa aaaaccagca gcgaaaaaga atagtgggac gtgataacta aaaacaaaag
     1621 gatacaattt gccagccccc aaaccaaggt gacctaaata tatagcaaaa ataccgagaa
     1681 attttaaagc atcaacccaa tcatgccttg tttttctgtt gttgtcgatt ttgttattat
     1741 acatttaaag taggctctcc agttttgtgt gattttcaaa cactcattgc aaatatgaat
     1801 gcctgaaagt ttatgattat taatcaacga gttcaatatt aatttattat ttttcaaagt
     1861 cgcactatat aggcatttac ttccgcagta ccaccgatag tatcgatatc aaatcagcct
     1921 gcggccgcga taactttcgt aacatcctgc cagcggcttt gttgaataaa tcaggttcaa
     1981 gacaatcgtc tctgcatcag gttagagtct caggcaatac acactctttc tggcatagct
     2041 gccactctcg caccatggcc atagccttta cgatctgcat actcaccaga ccagagcccg
     2101 ctccctttcg gggaggaatg agggctctga tattcttttg ccgtagttca tcgtgaaacc
     2161 gtcggatgtc atttggcctg aactaccagt ggtgtaggcc attcaatatc gtattatatt
     2221 gaaatataaa tatgcatcag ctgcacacgg tatatttcca tgccagaaga agtatcctga
     2281 cgtgaaacat gactttgttg tcaggatttc atgagagcgg ttcagcaaaa aatgacgaaa
     2341 atcctagtat agtcgggtga tgagcagtat agaggaatgt gttataacca ataaaagtcc
     2401 aattaaattt gtttttattt ctaagccccc cccaaaaaaa ggaggtggct tttaaattta
     2461 attcaaattt tataatggct cttggcaaaa tttgctacta tgttatttat tgcgattggg
     2521 gttgaaacaa caattgctgt tataagaagc aaatatggaa taaaataaat tctattacca
     2581 gaagcatata tgcttggtga taatcctaac atagatgttg tggcaatggt tccaataatc
     2641 attgtggata tgaaaattga agtgacatta aaattaagtg atattacggt tggaaatata
     2701 atgagtgctg taagtatcat ggcaaaagac accctaaaca cggagagtgt cgagaaaaga
     2761 ttgctttcag aaaaatgtat ggcttgaaaa ataactggcg gtgtatgtat aatatatagt
     2821 gtgatcaaga tacagcaaga aattagaggg agtatttttg tttttttctt gcatataata
     2881 gtactggaaa aagctagtaa tgctgggata attgatttta cagaaaataa catatctgca
     2941 tataagttaa gtccaagcat agatctttgg attatattaa aattaccata ctctttaaac
     3001 caatgttcta tctctgcata atacctgact ttatttcctg tgcaagttgc aatataaata
     3061 ataactaata gtgaaatccc catcgcattt aataatggtt ttgtattgta attgtttgtt
     3121 ttgcaaaaaa ttaacagtgt ggtgcataat aatacattga ctacagctat ctgttcattg
     3181 aatgatgata aaaaaaataa aaaataacaa ataacaaata acaaataaca aatagcaaat
     3241 tagagattga gttttctcgt ggttttccat attgattttt tttataagta gagaataacc
     3301 aataaatgca atagcacttg gccacagata gttgaatgat cctgttatcc aaatcgcggc
     3361 attgaaaaaa acatcgcttg gcatcataac aatcagtaat aatgacatga ttgaagtgtt
     3421 tattttatag taaaggttgt tttttctctg aaagatagag caaatgccaa taattaatga
     3481 tgaaaacatt gctgcattaa ttaaagacca agcataaaaa tgattaataa gatttattaa
     3541 tgcatattca atctatgttc gtgatgacca tgtgaaatat ctatctataa gccacttgat
     3601 gtgatccggt tctgatattg atttctcgga aaaccaaatg tcatctgata ctagtgtata
     3661 ttgatttaaa tgaagaatag ttaaaccaat aaaaaaagca aaagaaagca tatgtaattt
     3721 gatttaatca tttcttatcc ttaataatat atttaggtct attttttact tcaatatata
     3781 ttctgcctat atattctccg agaacaccaa tcccgatcag ttgcacgcca cccaagaaaa
     3841 gtattgatac aagcagggag ggatacccgc gtactgggtt accaaagaca agggtgtcta
     3901 taatcatcca tgcaccatat aaaaacgaaa tgcttgcaac aaacaagcct atataagtcc
     3961 atacgcgaag aggaaaggtt gaaaaacttg tgataccttc cagtgccaga ttccataatt
     4021 tccagccatt aaattttgag atgccagcaa cacgctctgc gcgtacatat tcaacgacat
     4081 ctgtctgacc acccacccag ctcagtatgc ctttcatgaa aagattgcgc tcaggcaaca
     4141 gtttaatgtt ctccacaacc tcacgagaca tgagtcgaaa atctccgaca ttttcctcga
     4201 tctttggggt gcttattttg ttgtgtaact tatagaacca ctcagctgtc ttacgtttca
     4261 gtcgtccatc agttgagcgg tctgaacgtt tcgcaagcac catgtcagca ccagcctgcc
     4321 acttttcaat aagatgaggg ataacctcaa tagggtcttg caggtcgaca tcaataggaa
     4381 ttacagcatc gccgcttgca tggtctaacc ctgcaaataa ggctggttct ttaccaaagt
     4441 tgcgtgtaaa tgacagcgga acaactagcg ggtctgaaac agccagcgcg ttaataattg
     4501 actctgtggc atctttactt ccgtcattta tgaatataat ttctacttca tatggcttca
     4561 actcttggaa ttcacgtacc gtcttataga aaacaggtat cgcttcttct tcattgaaga
     4621 caggaacgac aagagatatt ttcatttcgc atccctaaag acaatgaact ttga
//
LOCUS       DAETPZ010000190         4616 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.195, whole genome shotgun sequence.
ACCESSION   DAETPZ010000190 DAETPZ010000000
VERSION     DAETPZ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.195"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            6..81
                     /locus_tag="K0W59_004108"
     tRNA            6..81
                     /locus_tag="K0W59_004108"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="K0W59_004109"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="K0W59_004109"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="K0W59_004110"
     CDS             complement(299..1057)
                     /locus_tag="K0W59_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBI3024877.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQYFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="K0W59_004111"
     CDS             complement(1065..2168)
                     /locus_tag="K0W59_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBI3024878.1"
                     /translation="MTKVFLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLILASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3348)
                     /locus_tag="K0W59_004112"
                     /pseudo
     CDS             complement(2178..3348)
                     /locus_tag="K0W59_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3416..4441)
                     /locus_tag="K0W59_004113"
     CDS             complement(3416..4441)
                     /locus_tag="K0W59_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI3024879.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1269 a         1202 c         1097 g         1048 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tactgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaagaatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagcc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga caggaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cttggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcaata ttacggaaga tctcaatata aattgtggaa agctttcgta gcagccagtt
     3001 atccgaaagt ctcgccagac cgataaagaa gcccaaaaca gaagcgaaca caatacacaa
     3061 tgcagaaacc agtagcgtat tgagtaagcc gacaataaaa acgcgtccgt aggtgtcgcc
     3121 ctgctggtaa tcgatcaaat gctggacaat accgaagcca gcgccgcgat ccagaaaggc
     3181 aaaacctgaa gtaatgccac gattattgag attcgttaca gtgttttgaa ataaccaacc
     3241 aacaatgccg acaacagcaa caacggcaag gatctgaaat aaccaggcac gaaccgtagg
     3301 gttggcaaag gagagtgagc ctttaacggt tgagcggcga tgagacatag aaacctcgga
     3361 aagcataacg ctgaagagag cgccgccgta gcgacgctcg ttacctcaca ccgacttaac
     3421 gcacgggcgg cgcgtactga ataccgccgt tattccagag attattttgc ccacgtttaa
     3481 ttttcagcgg gctttctgaa cctacgttac gctcaaaaat ttccgagtag ttacccacct
     3541 gtttgatgat gttataggcc catttattat ccaacttcag atccttgccg taatcgcctt
     3601 ctttacccaa cagatgtgcc atatcaggcg ttgctggatt agccgctttt tcatcgacgt
     3661 tctgggaatt gatgcccatc tcttcagcat ttagcatggc gaaaagcgtc cagcgtacaa
     3721 tcgagaacca ttcatcatcg ccacgacgaa ctaccggccc aagcggttct ttagagataa
     3781 cctccggtaa gacaatccat tcagccgggt tgcttaattt gatgcgcaag gcatacagtt
     3841 gcgactgatc cgaagccagc gtatcgcagc gaccagattc cagtgccttc gctgattcgt
     3901 cagagcgatc gaaagtcact ggtgtgtact tcatattgtt tgccttgaaa tagtcggcga
     3961 cgttgagttc ggtatcagta cccgcctgaa tacagacggt agcgccatcc agttctttcg
     4021 cgctttttag ccccgcttta tcgtgcgtca ggaagccaat gccgtcgtaa taagtgacgc
     4081 cggtaaatgc cattcccatc ccggcatcgc gagatgaagt ccaggtcgta ttacgggaga
     4141 gcaaatccac ttcccctgac tgtaaagcgg tgaagcgttc ttttgctgtg agtggggtat
     4201 atttcacttt cgtgtcgtca ccaaatacag cagcggcaac accacgacaa acatcaacat
     4261 caatacctga aaacttaccg tctgcatcgg cataagagaa cctaggtaat ccatcactga
     4321 tcccgcattg cacaaaacct tttttctgaa cggcatcaag cgtcgcgcca gcatgcgcct
     4381 gatttgcaac ggcaagcagc acgctggcgg cagccagtgt ggctatcatc atctttttca
     4441 taatgctttc tgtgaggcga aaattatcgt tataggacgt ttgggaaacg tcttttacct
     4501 gtctgtggcc atcctcttat aagcaaaagt agtgccagct tttgcgcacg atttcgcgtc
     4561 gcaataagct gagtgtagac aggaaataat aaaaacagcg ccccgcaatg gtgcga
//
LOCUS       DAETPZ010000191         4609 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.196, whole genome shotgun sequence.
ACCESSION   DAETPZ010000191 DAETPZ010000000
VERSION     DAETPZ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.196"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..82
                     /locus_tag="K0W59_004114"
                     /pseudo
     CDS             1..82
                     /locus_tag="K0W59_004114"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin glycosylation ligase domain-containing
                     protein"
     gene            317..1225
                     /locus_tag="K0W59_004115"
                     /pseudo
     CDS             317..1225
                     /locus_tag="K0W59_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
     gene            1225..1959
                     /locus_tag="K0W59_004116"
     CDS             1225..1959
                     /locus_tag="K0W59_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HBI3024880.1"
                     /translation="MVNDLTYKKLQSTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRNERCNNMRTKQKV"
     gene            2427..3188
                     /locus_tag="K0W59_004117"
                     /pseudo
     CDS             2427..3188
                     /locus_tag="K0W59_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            3232..4311
                     /locus_tag="K0W59_004118"
     CDS             3232..4311
                     /locus_tag="K0W59_004118"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBI3024881.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMGYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQHFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4308..4595
                     /locus_tag="K0W59_004119"
     CDS             4308..4595
                     /locus_tag="K0W59_004119"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBI3024882.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQSATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQLLDLPLYFQTSVIT"
BASE COUNT         1262 a          885 c         1126 g         1336 t
ORIGIN      
        1 ctttccgtat ggcattttcc agcagcgaaa tgttcttgcg agctggctgg caacaggttg
       61 cggcgtagcg ttgtatctgg ctttaacggc gcgtactcgt tctctgacgt tgatatgttt
      121 gttgtcgtta taccctctgt ctacagcttt agtccttaca caaagccgtg tcggccttct
      181 tggtttattt tgcgtcatcg tgctggccgc tctcgcggac agaccccgct ggcgcggacg
      241 tccccttagc ggtctgttgc gcttgatctt cgtcgcctcc ttgctgtgct ggtgtgtcgg
      301 tatctcaaac tatgccatgc caggggagac aacggctgac tttacgcatt catcatcgaa
      361 tgaccagcgt atacgtattc tgaaaggtgc cataagtctt attcaacagc gccccttaac
      421 cggttggggg ttaggcagct ttgaagcgac cttcccgcaa gccttagagg atgtgggcgt
      481 gacaaacata gagagtgata cggctacgca tccccataac gaactgctgt tcgtaacggc
      541 tgaaggtggc atcgtcgcct tgtttggttt tttgctgcta tcaggcgttt ggctgtgacc
      601 gtgtatatgc cgcctgaaaa tgacaaacaa taacgcgcac catcaatgtg aaaagtggtt
      661 tattgcctta atggggttgc ctttagtcat tcatatgatg accgaatatc ctctctatac
      721 gtctgttccc catttgatgc tgttgctgct ccttttccgc atcggaatgc cggaaaacgt
      781 gatgaatcag gtacgtatac ctaaaacagt ccggttgggt attctgcctg tcattgtgct
      841 cattatgacc gccgcgctgg tcatcctggt agcgggattt gatgcacagc gggaactgac
      901 cagagctgaa gctgatatga acgaaggatt tatccctcgc cttccggaac atagctggaa
      961 atacctgacg caggcagaac gtcttgagcg cgatcagcat ctgcttattg cgaataaacc
     1021 ggggttcgtt cgcgatacgg aaagcatgtc tcttttcact gtgtggggaa ataaatggtt
     1081 ggcaatccac aatgatgcag acgtaagtgc tgcaatgata ttcattgcgc acaggcgcgg
     1141 tgattatctg acagctgaaa aaataagaaa acgcgcagct cgggtatttg taaatgacga
     1201 acgttttact cgcgggggaa actaatggtt aacgatctga cgtataagaa attacaaagc
     1261 acaaacatca actgtaatat tctctcgcca gaaaatacca catggacaaa tgtcacggtg
     1321 attggcctgg atctttttgc catttgcggc gtgtgcagtt tgctcaagcg tgttggggga
     1381 gtgaaacttc aggttttcac ttccttaccc actgatatga atgttctgac tgacagtagc
     1441 atcgtgatat gggtaaaaat gcgtcacgat ggtttgcctg aattagccgg acatattgtg
     1501 aaggtttgtc gtcgataccg tctgatgcgg caactcgtta ttagcgatgc tattcctgaa
     1561 ggaatgacga atgatatcgg gcctctttcc cgagtatggc ttactcatgg gagtgcaaaa
     1621 gtggatattt tattatcttc attacggaag gtgttaaatg ctcattgttt gcctggtcct
     1681 ttgtttataa aacgcctggg aagagtgcaa tggagggttc ttttactccg cgcgaagggg
     1741 attagcacac gtaaaattgc agaaatctgt ggtattggcc tcaagaccgt tagcgcccat
     1801 gagtccgcaa ttcgagaacg attaggcacc aggaataaaa gtgagtacgt ctggttagta
     1861 cgtaatgctc aacttataca acaggcgata cccgcgttgg gaagagatgc gtcaaggaat
     1921 gaaagatgta acaatatgcg aacaaagcaa aaagtataat tgatgcgtca tagataactc
     1981 tctttttcga tgacgaaatc gctgagcatg cgtggtgagg ttagtaatag atgggtgcta
     2041 tgttttgcca cgggtttgta gctgtgaaac ctggaaaaaa ataagaatta aaaatattta
     2101 aagagagtta ataaggttct ggttgtaatt gtcttggtca aaaaaaagat attgtcaatg
     2161 ttgtttttta aattttcagg ttttggttaa atatttgctt acaaatatag gtgatttttt
     2221 tattgtaggc ttctgaaggc tattataact gtgcgctaaa ttaatgcgtt gaataaaaga
     2281 aataacaaaa ttaagaaaag ccatatttac gatatgggca acataaatat aatctaaaaa
     2341 agagtaaggc gttgtaggtt ggttccgcgt tcattacctt gttagtgaac gcggatttat
     2401 cagttgtcga aatggttttt ataataatga aaaattttaa tgtactaacc aaagtcgtat
     2461 tttgcttgtc gtttttatca agcgcatttc ccacctatgc tgcggatgat gtcaaagtga
     2521 ctcaaaacac ttcagttttt aaactgaagc ttagtgcaac gcgattaatc tatgaacaag
     2581 ggtcggatgg cgcagcgata actgtgacaa atccgcagga ttggccgatg cttgtgtaga
     2641 gtaaagtctt tgaagaagat aaagtaactg cagcgccctt tattgtcact ccgccactgt
     2701 tcaggcttaa tccaggccag caaaataaaa tgtgggttgt gcagactgcg ccagctttac
     2761 aagacaggga aaaattacac tggctatgcg ccaccggcat tccgcctaaa aagagtgatg
     2821 tttgggccga gaaagataaa gaaaaaattg ctgaaaatgt gaacctgaac atccaggtta
     2881 acgtacatac ctgcataaaa cttattacca ggccatctgg tattgaaggc aagatggtta
     2941 atgcgagtga gaagttatcg tggaaaaagc agggtgataa aattgtcgcg gtcaatcagt
     3001 cgccgttcta catcaacctt tcttctttac aggttggcgg tagcaagatt gagaaactag
     3061 attatattgc gccgttttcg caacatgagt ttgttatgcc aaaaactagc ggccaggata
     3121 ttaaatggtc tgtgattact gatgaaggtg gtgagagtcg tttgtttaca tctcatttgc
     3181 aatcataatg tattacattt ttattgaata tttttatgcc atgcatattc catgttttat
     3241 cgactgtcat taattttgat aatggcctta cttgcgggtc agttaagtgc ccaggagtgg
     3301 tcatttgatt cttcacagtt ggaaggaaac gttagtgcgg acactgtggc aatgtttaat
     3361 cagggagagc aactccccgg aaattatcgt gtggaaattt atcttaacgg tgaaaaagtt
     3421 gatgttgggg agtttccctt tcatcgtccg gaatcccctg aagaaaaaga gcttgttcca
     3481 tgtttaaccg ttgatgacct tatccactat ggcattaaaa ttgataaaag cagcagtgat
     3541 actgacaata aaaagaatca gtgttttaaa tggaactcta ttgaaggttt gaaagtaaat
     3601 tatgattttg atagccagcg cgtacagata accgttccac aactgtatct gcaagataaa
     3661 aaaagtagtt tagcaccagt gtcgttatgg aatgagggtg tagctgcatt ccgcatgggg
     3721 tatcagacaa atattgatat ttccaaacaa aatgataatc aatctaccac gcgtaattct
     3781 cgttatggca ggttcactcc agggtttaat cttggtgcct ggaggtttcg tagttcagtg
     3841 acatggagca aggaacttgg ccagtcagaa agatggcaaa ggggttacat gtggtttgag
     3901 cgaggcataa atgcgattaa aagccgatta acgcttggag aatcttatac gtcgtctgag
     3961 gtgtttgaca gcataccttt tcgcggcggg atgttagcga ccgacgatgc gatgactccg
     4021 ccggaggaca gctactacac acctgtggtg catggaatag cacaaagtga ggcgcaggtt
     4081 attattaaac aaaatgggca aattattttt acccgttctg tgccgccagg gccttttgcg
     4141 ctggataatt taccaacgct ggccgttggc ggggagcttg atgttacagt gagagagagt
     4201 aatggcgagg agcagcattt ttccgtgcca tttcaaacgc cggccatcgc tctgcatgaa
     4261 ggatatttca aatatagtgt gatggggggg aatataaaga agaaggtata aaaagctccc
     4321 ctgtctccgt gggcgaaatc acggcgatgt acgggcttcc ttatggcgtc acggtgtatg
     4381 gagggattca aagcgccact cattttaatg caatatctac gggtattggc atttctctgg
     4441 ggctgttagg gagtttatcg acagatatta cgcgttcgat tgcgaattta tattatggga
     4501 ataaatatcg tattcgttat agtaaatcca ttagtgattt tggtactcaa ttgttggatt
     4561 tgcccctata tttccagaca tctgttatca cttaacccat tacaagccc
//
LOCUS       DAETPZ010000192         4549 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.197, whole genome shotgun sequence.
ACCESSION   DAETPZ010000192 DAETPZ010000000
VERSION     DAETPZ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.197"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            132..575
                     /locus_tag="K0W59_004120"
     CDS             132..575
                     /locus_tag="K0W59_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBI3024883.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQAHLGHRSSSMTDKYRDGRGREWKDI"
     gene            811..1630
                     /gene="ybhA"
                     /locus_tag="K0W59_004121"
                     /pseudo
     CDS             811..1630
                     /gene="ybhA"
                     /locus_tag="K0W59_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
     gene            complement(1631..2689)
                     /gene="modC"
                     /locus_tag="K0W59_004122"
     CDS             complement(1631..2689)
                     /gene="modC"
                     /locus_tag="K0W59_004122"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HBI3024884.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2692..3381)
                     /gene="modB"
                     /locus_tag="K0W59_004123"
     CDS             complement(2692..3381)
                     /gene="modB"
                     /locus_tag="K0W59_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBI3024885.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3381..4154)
                     /gene="modA"
                     /locus_tag="K0W59_004124"
     CDS             complement(3381..4154)
                     /gene="modA"
                     /locus_tag="K0W59_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI3024886.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKANEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="K0W59_004125"
     CDS             complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="K0W59_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HBI3024887.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
BASE COUNT         1233 a         1188 c         1084 g         1044 t
ORIGIN      
        1 ttgccgaaga agatccagct tctgcaacca ggccattcag aactgaagtg aagcgcagtc
       61 gactgttaat tgacgagtat ctgttaatca ggaaaatagc agacgtccag aacgaatggt
      121 tcggcctttg catggatctt gcactggtaa caggacagcg cgaaggagat ctggcggcta
      181 tgagatggga ggatataaga gatagcaggc tatacgtgga acaacaaaaa acaggggcaa
      241 aaattcgcat ttctctcccc accacgattt ccagattgaa tttaacactt gccgatgtac
      301 tggataactt gaaaaaaata aacggtaaaa acgaaaagtt actcggaggg aagacagcca
      361 ggacaatcgc agcacagttc aggattgccc gagacacatc cggattgaaa tgggaaggtg
      421 acccaccgcc atttcatgaa atccgaagtt tatccggtcg cctgcactca gcagaaaaag
      481 gcagtgattt cactcaggcg caccttggac atcgttcatc cagcatgacg gataaatacc
      541 gtgacggacg agggagggaa tggaaagaca tttagaatta atttcgaatg atttcgaaat
      601 actacttgta atcaattgat tataaaggat taattcctaa agagcatttg cttcatgaat
      661 gctcctttgc atttagcaac tgactgtaac agctaaaatt agtcgctttt ggcggtgaat
      721 gctgaattaa aacgcagaat tttgtccgat agtggtatcg gtgtaccatt cgcgcagacg
      781 aaaattctta accttaatct ggaatacgcc atgaccacac gcgtgattgc tctcgactta
      841 gacggcacct tattgacccc gaaaaagacc ttgcttccat catcgataga agccctggcc
      901 cgcgctcgcg aagcaggcta tcaattaatc atcgtcacag gtcgccatca cgtcgctatt
      961 catccttttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     1021 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     1081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     1141 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     1201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca
     1261 acaagttaac gccgtatgga agttcaccct gacgcacgat gacctgccgc aattgcagca
     1321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     1381 ggttgatatt gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     1441 gcaaggttgg tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat
     1501 gctggaagcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     1561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     1621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     1681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     1741 cgacttccag ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     1801 gcaatacgtt acgaatgctg gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     1861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat
     1921 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca
     1981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc
     2041 acacttcctc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     2101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt
     2161 cccgtgtcag ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     2221 ccagcggttc atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca
     2281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     2341 ctaaaagcgc caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca
     2401 gattgccacg cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac
     2461 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga
     2521 ggacaatccg ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     2581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     2641 tggtcaggca atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc
     2701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     2761 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     2821 ggtatacatg gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat
     2881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     2941 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt
     3001 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     3061 catcagcgga aacgacatga cggcggcagc gagtaccgcg ccgcgccagc taaaggcgaa
     3121 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac
     3181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     3241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     3301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     3361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     3421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     3481 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     3541 tttacccctt tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta
     3601 cgttcgacca gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc
     3661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     3721 tccggatcgc caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg
     3781 tcgatggtga aatctttctg ctcgttggct ttcggtgcta caacgaccag gctattgccg
     3841 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat
     3901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     3961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     4021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     4081 tctgccagtg cattaccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac
     4141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     4201 aactcggaat tttcccagct gttattgcta acctttgtga ggtagataag aaaaaatatc
     4261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc attcagggaa
     4321 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     4381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     4441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     4501 tctaaccgct aagcacagaa aagggtaatg ctgccaactt actgattta
//
LOCUS       DAETPZ010000193         4439 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.199, whole genome shotgun sequence.
ACCESSION   DAETPZ010000193 DAETPZ010000000
VERSION     DAETPZ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.199"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..213)
                     /locus_tag="K0W59_004126"
     CDS             complement(<1..213)
                     /locus_tag="K0W59_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HBI3024888.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="K0W59_004127"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="K0W59_004127"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBI3024889.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLGGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="K0W59_004128"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="K0W59_004128"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HBI3024890.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="K0W59_004129"
     CDS             2894..3634
                     /locus_tag="K0W59_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI3024891.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="K0W59_004130"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="K0W59_004130"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBI3024892.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(4192..>4439)
                     /locus_tag="K0W59_004131"
     CDS             complement(4192..>4439)
                     /locus_tag="K0W59_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024893.1"
                     /translation="KFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFLGSI
                     PWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1099 a         1105 c         1233 g         1002 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcag cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cggcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctgggagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagac atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggtcaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgatgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtctt acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctgg cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tctggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttg
//
LOCUS       DAETPZ010000194         4327 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.200, whole genome shotgun sequence.
ACCESSION   DAETPZ010000194 DAETPZ010000000
VERSION     DAETPZ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.200"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..324
                     /locus_tag="K0W59_004132"
     CDS             <1..324
                     /locus_tag="K0W59_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HBI3024894.1"
                     /translation="DDALLQLREFIDENSGEFFVQVWGNGANFDNTILRRSYERQGIP
                     CPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIP
                     SQADF"
     gene            383..586
                     /locus_tag="K0W59_004133"
     CDS             383..586
                     /locus_tag="K0W59_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HBI3024895.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENGLPFYTNRSGKPIV
                     SRELFTCNKTLPPHEVEPNFGAI"
     gene            586..1623
                     /locus_tag="K0W59_004134"
     CDS             586..1623
                     /locus_tag="K0W59_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBI3024896.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPSNSKCDFCH"
     gene            complement(1651..1740)
                     /locus_tag="K0W59_004135"
     tRNA            complement(1651..1740)
                     /locus_tag="K0W59_004135"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1704..1706),aa:Ser,seq:cga)
     gene            1834..2631
                     /gene="mtfA"
                     /locus_tag="K0W59_004136"
     CDS             1834..2631
                     /gene="mtfA"
                     /locus_tag="K0W59_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HBI3024897.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2732..2807
                     /locus_tag="K0W59_004137"
     tRNA            2732..2807
                     /locus_tag="K0W59_004137"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2765..2767,aa:Asn,seq:gtt)
     gene            2969..4231
                     /locus_tag="K0W59_004138"
     CDS             2969..4231
                     /locus_tag="K0W59_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBI3024898.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHLELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGSELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
BASE COUNT         1207 a          996 c         1036 g         1088 t
ORIGIN      
        1 gatgatgcac tgttacaatt gcgggaattt atcgacgaaa actccggtga attttttgtt
       61 caggtctggg gaaatggagc caacttcgac aacacgattt tgcgccgttc atacgaacgg
      121 caggggatcc cctgcccgtg gcgttactac aacgatcgcg atgtacgcac aatcgttgag
      181 ctggggaaag ccatagactt cgatgccaga acggctattc cattcgaagg tgagcgccat
      241 aatgcacttg atgacgctcg ttaccaggca aaatacgttt cagctatctg gcaaaaactg
      301 atcccgagtc aggctgattt ttaatgttca accctaattg ccgctaaccg tatatagtta
      361 gcggcggtta tgagatatag ctatgagcag cttattttta accgaagatg aattgctaat
      421 attaacgggc tgcaaatatg caagccacca gcgaaaatgg ttaatggaaa acgggcttcc
      481 gttctatacc aatcgtagtg gcaaaccgat tgtcagccgg gagctattta cctgcaataa
      541 aactttacca ccacacgagg tagagccgaa ttttggtgcg atctgatggg aagacgaagg
      601 aaaaatcctg aacacgaaaa attacctcca aatgtatacc caaataaata tagttatgta
      661 tggaaaccaa catccagaga atctgtaaca ctaaccgcca tcaaggatgg tttagctgct
      721 ttatggaaaa agtatgagga aactgtaaat aatcgcgatc gtgcaatgac attcggtcgc
      781 ttgtgggaaa aattcctcgc cagcgcctat tacagtgacc tcagtccaag aacacaaaaa
      841 gattatctgc aacatcaaaa aaagttgctt gccgtattcg gtaaggtgcc ggcagattcc
      901 ataaaaccag aacacatccg tcgatacatg gacaagagag gggaacagag taaaacgcaa
      961 gccaaccatg aaaaaagcag tatgtcccgc gtttacagtt gggggtatga gcgagggtac
     1021 gtgaaggcta acccatgtgc aggtgtaagt aaattcaagg ccaaaaaccg cgaacgatat
     1081 gtaaccgaca aagaatacca ggcagtatta agcgttgcac ctcttcctgt ttttatcgca
     1141 atggaaattg cctatctgtg tgcagcgagg gtttccgatg tgttatcgct gaaatgggag
     1201 cagattggaa acgacgggat ctttatccag caagggaaaa caggaaaaaa acagataaaa
     1261 gcatggagtc cacgattaca ggcggcgatc gaaaaagcaa aacagttacc aacatccgcc
     1321 tatgtaatca gcaatcaata cggcaaccga tatatgtaca aaggctttaa cgaaatgtgg
     1381 gtagaagcaa gaaatcgcgc aggcaaaatt tcaggtattt taaccgactt cacctttcat
     1441 gatctgaagg cgaaaggaat ttcagactat gaaggaagca gtcgggataa gcaacttttc
     1501 tctggtcaca aaaccgaggg gcaagtgcta atctatgaca ggaaggttaa agtttcaccg
     1561 acacttgatg tcccgttacc tgaaaatatt ccaagtaatt ccaagtgtga tttttgtcac
     1621 tgacttaatg atgtataagt gattgaattt tggcggagag agggggattt gaacccccgg
     1681 tggaggtgcc cccactccgg ttttcgagac cggtccgttc agccgctccg gcatctctcc
     1741 gctcagatgg ttgccatgat gccaggaaat ttggcatttt aacagtccct gtccgtgcaa
     1801 ttttgttcaa gtgacgagtt tgcgagcaaa acgatgatta agtggccctg gaaagtacaa
     1861 gaatcagcac atcaaactgc ccttccctgg caggaagcac tatcgatccc ccttttaacg
     1921 tgtctgacag aacaggaaca aagcaaattg gtcgctcttg ccgaacgttt tttacagcaa
     1981 aaacggcttg ttcctttaca gggctttgag ctgaattcat taagaagctg ccggatagca
     2041 cttctatttt gcctgcccgt tctggagtta ggactggaat ggctggatgg ttttcatgaa
     2101 gtcttaattt atcctgcgcc atttgtggtc gatgatgaat gggaagacga tatcggtctg
     2161 gtgcataacc aacgtattgt tcagtcaggt cagagctggc agcaagggcc tatcgttttg
     2221 aactggttgg atatacaaga ttcttttgat gcttctggtt ttaacctgat tattcatgaa
     2281 gtcgctcata agctggacac ccgtaacggc gatcgcgcca gcggagttcc ctttatttcg
     2341 ttgcgtgagg ttgctggctg ggaacacgat cttcatgctg caatgaacaa cattcaggaa
     2401 gaaatcgaat tagttggtga gaatgcggcg agcattgatg cttatgctgc cagtgatcct
     2461 gctgaatgtt ttgccgtact ttctgaatat ttctttagcg ccccagaact ttttgctcct
     2521 cgtttccctt cattgtggca acgtttctgt caattttatc aacaagatcc tttgcagaga
     2581 ctgcatcacg ctaatgatac agactcgttt tcggcgacga atgttcatta attaacaact
     2641 ttgcagatta attaaccaat tgaaatggct tatgaaattt agtgttgaca gacaaggtac
     2701 cgctaagtaa tatgcgcccc gttctcacga ttcctctgta gttcagtcgg tagaacggcg
     2761 gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaatt ttctgttttc
     2821 atgcatcctt gcgaatcttt atctgatgtt gattcaacag gttagtgtga aaactcttct
     2881 cggtgcattt tgattttacc ctacgcatcg ggaaaaattg gtggtcaaat ctgggatcag
     2941 gttagttcga taatggagtg acccccatat atcccttacc gacgcaaaaa tccgcaccct
     3001 caagccttct gataaaccct ttaaagtctc cgattctcac ggtctgtatc tgctggtcaa
     3061 gccgggtggc tcccgccact ggtatctcaa ataccgtatt agcggtaaag aatcccgcat
     3121 tgcgctgggt gcctatccag ccatctccct gtctgatgcg cgacagcaac gtgaaggtat
     3181 ccgtaaaatg ctggcgctga atatcaaccc ggtacagcag cgggctgctg aacgtggctc
     3241 acgaacaccg gagaaagttt ttaaaaacgt ggcgctggcg tggcataaaa gtaacaggaa
     3301 atggtcgcag aacaccgccg accgtctgct tgccagcctg aacaatcaca tctttccggt
     3361 catcgggaac ctacctgtat cagaacttaa accccgtcat ttcattgacc tgctgaaagg
     3421 gatcgaggaa aaaggtctgc tggaggttgc gtcccgcaca cggcagcacc tgagtaacat
     3481 aatgcgccat gcggtccatc tggagttaat cgatacgaac cctgcagcaa accttggcgg
     3541 cgtgaccaca cctcctgtca gacggcacta tcctgccctg ccgctggagc ggctgcctga
     3601 actgcttgaa cgtattgggg catatcatca gggcagtgaa ctgacccggc atgccgttct
     3661 gctgatgctg catgtgttca ttcgctccag tgaactgcgt ttcgcccgct ggtcagagat
     3721 tgatttcaca aaccgagtct ggacgatacc cgcgacgcga gaacccatta ttggcgtgcg
     3781 ttattccggc cgcggggcaa aaatgcgaat gccgcatatc gtccccctct cagaacagtc
     3841 catcgccatt ctgaaacaga ttaaggatat caccggtaat aatgaactga tcttccccgg
     3901 cgaccataac ccgtataagc caatgtgtga aaacacggtc aataaggcac tgcgggtgat
     3961 gggttacgac acgaaaaagg atatctgcgg tcacggcttc cgggcaatgg catgcagtgc
     4021 gctgatggaa tcgggtttat gggcaaagga cgcagtagaa cgccagatga gtcatcagga
     4081 gcgcaatacc gtgcgcatgg cttatattca taaggcagag cacctggaag cccgcaaagc
     4141 gatgatgcag tggtggtcgg attatctgga agcatgccga gaatcttatg caccgcctta
     4201 tacaattggt aaaaataagt ttatcccata gtatactaac gaatcccaca ctctggcaaa
     4261 ttaccaccac ctctacagga ggtggttcta tatagacttt tatcaacttt atgacgaaaa
     4321 acaccta
//
LOCUS       DAETPZ010000195         4327 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.201, whole genome shotgun sequence.
ACCESSION   DAETPZ010000195 DAETPZ010000000
VERSION     DAETPZ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.201"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..157)
                     /locus_tag="K0W59_004139"
     CDS             complement(<1..157)
                     /locus_tag="K0W59_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024899.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASK"
     gene            complement(402..932)
                     /gene="cybB"
                     /locus_tag="K0W59_004140"
     CDS             complement(402..932)
                     /gene="cybB"
                     /locus_tag="K0W59_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HBI3024900.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1121..2122
                     /gene="gap"
                     /locus_tag="K0W59_004141"
     CDS             1121..2122
                     /gene="gap"
                     /locus_tag="K0W59_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBI3024901.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATKTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(2164..3603)
                     /gene="aldA"
                     /locus_tag="K0W59_004142"
     CDS             complement(2164..3603)
                     /gene="aldA"
                     /locus_tag="K0W59_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBI3024902.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3800..>4327)
                     /locus_tag="K0W59_004143"
     CDS             complement(3800..>4327)
                     /locus_tag="K0W59_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBI3024903.1"
                     /translation="RTTGRAEATIQVDIAHQFWHIPHEKIWIEDQSTNCGENARFSIT
                     LLNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSV
                     IFINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLK
                     HDAVLIEAMESRSLR"
BASE COUNT         1143 a         1083 c          994 g         1107 t
ORIGIN      
        1 ttttcgatgc actaatagaa ccatcattac agacaaattt gccgcctgcc gtacagtgtg
       61 agatcccccc tttcgaacca gaacagggtt tgcgcccggc atcggcataa aaggaaacaa
      121 gcgttcccat aaacaaaata agtgccagtt ttttcatata acctccatgg tttatcgaat
      181 cttatgtgcg atttgaacgt tatacgctgg tttattttga taccgttgat gagatcaaag
      241 atcgggtgaa ttgattaaaa ataagccaga tggacgtatg ggcaaggatt atggttttat
      301 ttgtcataca aataaatatg ataggcgctt ccgatgtaga cccgtattct tcgcctgtac
      361 cacgggtcgg tttttgtaca ggcgttttct ttaaatatcc ttcaggaacg tttacgcggc
      421 atcatgcgta gaagtgtgtt gtccttccag aaatagtggt gtgccagtgc agccgcagcg
      481 tgcaacccga tgacaaaata tcccagattc gccagcgtct catgccacga ctttaagcta
      541 tcaacccgtt cgaaattggc ctctgaagcg taaggcatcg tcaaaccaaa cgcaaaccac
      601 gggttgcccc ggttatacat catcaccaaa ccaatcactg gcagcgcaat aaacaggaga
      661 taaatcacca aatgtcccaa atgcgccagt cccgtcatca tcggtttcgg cttaggtata
      721 atcggcgggg ttgggtattt cagtcttaac aaaagacgaa ccaccatcag cacgagaatt
      781 gagatgccac aggaaacatg aatcatgttg atgagcggcc gatcgctacg tgggaagaaa
      841 ccacgaaact ccattgcgca atacgctgcg ataaccagca aaaagaccag ccagtgaatg
      901 ctgatttgta accttgagta tttattttcc ataacatttc ctgctttaac ataattttcc
      961 gttaacataa cgggcttttc tcaaaatttc attaaatatt gttcacccgt tttcagtacg
     1021 ttacgtttca ctgtaagaat ggagattgcc catcccacaa tcctggtcta agcctggaaa
     1081 ggatcaattt tcatccgaac gttcctgaca ggagaaaacc atgagtaaag ttggtattaa
     1141 cggttttggt cgtatcggtc gactggtgtt gcgtcgttta cttgaagtca aaagcaacat
     1201 agacgttgtc gctattaatg atcttacttc cccaaaaatt ctcgcctatc tgctgaaaca
     1261 tgattcaaac tatggtccgt tcccctggag cgttgatttt acggaagatt cacttatcgt
     1321 tgatgggaaa agtatcgcgg tttacgccga aaaagaggct aaaaatattc cgtggaaagc
     1381 gaaaggggca gaaatcattg tcgaatgtac tggcttttat acctccgccg ataaatcgca
     1441 ggcgcatctt gatgctggtg cgaagaaggt gttgatttcc gctcctgccg gtgaaatgaa
     1501 aaccatcgtt tataacgtta atgacgacac tctggatggc aacgacacca ttgtttccgt
     1561 ggcgtcatgc accactaact gccttgcgcc gatggccaaa gccttgcatg acagtttcgg
     1621 gatagaagtc ggcacgatga cgaccattca tgcctatact ggcacccagt cactggtgga
     1681 tggcccgcgt ggtaaagatt tacgtgcttc acgcgcagcg gcagaaaata tcattcccca
     1741 cactacgggg gcggcaaaag ccattggtct ggtgatcccg gaactgagcg gcaaactgaa
     1801 aggtcatgcg caacgcgtgc cggtgaaaac aggttcggtc actgagctgg tgtccattct
     1861 cggaaaaaaa gtgactgccg aagaggtgaa taacgcactt aaacaagcaa ccaccaataa
     1921 cgagtcattt ggttataccg atgaagaaat agtctcttcc gatatcattg gcagccattt
     1981 cggttcagtg tttgatgcca cgaaaacgga aattaccgcc gtgggcgatt tacaactggt
     2041 gaaaacggtc gcctggtacg ataacgaata tggcttcgtc acgcagctta ttcgcaccct
     2101 cgaaaaattc gctaaactct gacgcgcaca ggcggaggaa aaaacctccg cctctttcac
     2161 tcattaagac tgtaaataaa ccacctgggt ctgcagatat tcatgcaggc catgtttacc
     2221 atctgcgccg ccaataccgg atttacgcca tccggcgtgg aaaccttgca tagcttcgaa
     2281 gttttcacgg ttgatgtaag tttcaccaaa cttcagccct ttaatggctt tcatcgcgac
     2341 gttcagattt tgggtataga ttgatgaggt caggccgtaa tcactgtcat tagccattga
     2401 gatagcatct tccagcgtgt caaatgcgac aactggcagc accgggccaa aggtttcctc
     2461 atgcataatc gacatttcct ggcgaacatc cagcagcaat gtcggcggat aataatatcc
     2521 tttcccctct accgctttgc cacccaacgc cactctcgcc ccttcttcta ctgcgcgcgc
     2581 cactttttgc tcaacccttt ccagcgccgc ggcgttaatc aacggcccca tcgcaatgtc
     2641 gttgcgttca gcggggttac caaattgaac cgcctgcaac gcttcaccca gccgattgac
     2701 gaactgatca taaatgcctt tctgtacata aacacgttct gcacagttac acacttgccc
     2761 actattaatg acgcgtgaat caacgatggc tttgactgcc agttcaagat cggcatcgtc
     2821 cattacgata gccggtgctt taccacccaa ttccagacac actttggtga tgtttttcgc
     2881 cgcagtcgcc atgatcttct cacctgcaga gacgctgcct gtcatactga ccattgcgac
     2941 ctttgggtta cccgccagtt cttgcccaac ggtttcacca cgccccagta caaggttaaa
     3001 cacgccgcgc ggaaggccta tttcatcgac gattttggcg aatgcaatcg cattgtttgg
     3061 cgtaaattca ctgggtttaa tgacgatggt attaccggtc aaaagagcgg gagccatttt
     3121 gcgggcaatg aggaagaacg gaaagttcca cggcagaatg ccggtagtca caccaagcgc
     3181 acgtttaaac agaagaatat tttctcctgg acgatcgctt tgaataatct cgccctcgta
     3241 acgccgtgcc cactccgcca tgtaatcgat atagtcggca gtaaaagcca cttcgacttc
     3301 agctagctgc tggatcttgc ccccttcttc aacaatcagc gcactgattt cactggcgcg
     3361 ttcgcggatc ccggcggaga ttttgcacaa ccaactggcg cgttcaatag caggcaacgc
     3421 ttcccattct ggttgtgcac gttctgctgc atcgattgcc ttacgggcat cctcggcctg
     3481 accatcgggt atgcgggaaa tgacagcctc tgtagcaggg tttaccacat caatccatgc
     3541 gtctccacgc caggtaacaa actgtccatc gatatacata ggatgttgaa cgggtactga
     3601 catgggcgac tcctgtgatt tatatgtttg ttttcggtga atatattgtt aattaacatc
     3661 aatcatcaga gagacggcat cgggaaagga caattctgtg gagggcttca taaaaacaag
     3721 tgtaaaagga ataaaaggca gaaataaaag tcaggagtca tgcccgtttg caaacaagtg
     3781 gttcacaaac gggcaatttt taacgtaacg agcgactttc catcgcctcg atgagcaccg
     3841 catcatgttt cagcgtttgc catgcatgga tgacttctgc cggaaaatca acgtgaacga
     3901 taaaatctcg cccgcgggga ccgtagccat cgctatcatc gtgtaaacgc ggcagctcgc
     3961 cagtgagtag tgagagataa cgctcaaccg gccataatcc ttgtaactga ttaataaaga
     4021 ttacactgtc tgcgttattt cctaactgag gaacgaatcc gggataactt aaccagcgtg
     4081 gtgcatcggg attgtccccg gtcatacggc ggaacgttgc catcgtgcgc cgctgcatgg
     4141 tggggtcctg tacaacgata gccgtatgaa ctcgttctac ggcctgattc aatagcgtga
     4201 tgctaaagcg tgcgttttca ccgcagtttg ttgactggtc ttcaatccag attttttcat
     4261 gcggaatatg ccagaactga tgagcgatat ccacctggat ggtcgcttct gctctgccag
     4321 tggtgcg
//
LOCUS       DAETPZ010000196         4300 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.202, whole genome shotgun sequence.
ACCESSION   DAETPZ010000196 DAETPZ010000000
VERSION     DAETPZ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.202"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..301)
                     /locus_tag="K0W59_004144"
     CDS             complement(<1..301)
                     /locus_tag="K0W59_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3024904.1"
                     /translation="MTDITANVIVSMSSQLFTMARSFKAVANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHVPVSQPIVINAAGYPVYNGQIAKFVTEQGHSMAVYDAYGSQQ"
     gene            complement(433..2274)
                     /locus_tag="K0W59_004145"
     CDS             complement(433..2274)
                     /locus_tag="K0W59_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HBI3024905.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     APTREEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDRPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDALHEVLQQTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNMLLRLANTKAGTAAYERALNNAANAIRPILSSQITAEQ"
     gene            complement(2274..3680)
                     /locus_tag="K0W59_004146"
     CDS             complement(2274..3680)
                     /locus_tag="K0W59_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HBI3024906.1"
                     /translation="MATWQGSNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQENQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEDQRNSIGTLAAGARLAASSPEAMQSWLQNNANELARVGVGPNSVA
                     QMYQQNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALQ
                     ARGQNLSYQSAMTGHNIAAQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNMEKGI
                     ETAVNNATASSNSANSMSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLRIRQ
                     NALVNSQVLKFLPPGPATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQFNE
                     FKAEWMSANGNPGQSRNGGQILGLDVKKGESLGSAVKRYMSMNTDAAPAQDSTPSGEP
                     RNQVGSYTSKSGIQFTVE"
     gene            complement(3690..>4300)
                     /locus_tag="K0W59_004147"
     CDS             complement(3690..>4300)
                     /locus_tag="K0W59_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HBI3024907.1"
                     /translation="NTLQETRGGSQKYAADLQNQQWQTIMKNLTPFTPLAEQYVNQLQ
                     NLSSLEGQGQALNQYYNSQQYKDLAGQARYQSLAAAEATGGLGSTATSNQLATIAPTL
                     GQSWLSNQMSNYNNLANVGLGALQGQANAGQTYANNMSSIAQQSAALAAANANKPSSL
                     QTAISGGTSGAIAGAGLASLLGTSTPWGAGIGAGIGLLGSLF"
BASE COUNT          990 a         1094 c          966 g         1250 t
ORIGIN      
        1 actgttgcga accatacgca tcatatacag ccattgaatg gccttgctca gttacaaatt
       61 tggcaatctg cccgttgtac acaggatatc cggcagcatt gataacaatt ggctgagaaa
      121 cagggacgtg agatccgtct tcattctcta cataaacctg aatctggttt tcaggattta
      181 ccgggtcagt gtcaattttt ccgatataaa ttttgccatt ggctacggct ttaaaagaac
      241 gagccatagt gaagagttgc gaagacatcg atacgatcac attggctgta atgtctgtca
      301 tttaatttgc tccagataca atgaatcgcc gcagcgtggc tacggtgaat tttgggcata
      361 aaaaaaccca gccgaagctg ggtagttgcg ttggttatct gtcagtagtt atgtgcttat
      421 ttaattattt atctattgtt ctgctgtaat ttggcttgaa agtatagggc gtatcgcatt
      481 tgcagcatta tttaacgctc tttcataagc tgccgttcct gctttggtgt ttgccaaacg
      541 aagaagcata ttccttgcgg ctttggactc atacaaacgc atcattgcac caaaaccagc
      601 ctcaagcccc attgatacac caagagtcgc agttgcgcca atcgtcctta tcctgttggc
      661 ttgcgattgc cccgtctgag ttactacatt tgcggtgtct gaccttgctg tttgctgtag
      721 aacttcatga agagcatcaa gctctttcat gtgctttcca gaaaaaatag tgttgtaaat
      781 ttcaccgcct gactgagatt tcagcttatt aacttcagtg atgaacttgg ctggagagtc
      841 accggccttt tccgctattt tgctgacgta agctgcacgc atagcatctt tccctttatc
      901 atccagtgcg ctccagattc gtttcacgtc agatggtttt ctgcttaata caacggtatt
      961 tataagttca ggactggctt cactgcttgc cttgttgagc ttgttagcaa tgtttttatt
     1021 aagcacctta ttataaacgt ttgcataatc ggaatttgct ttaaggtatt ttgctgcgtc
     1081 tgatgcaccg aggtttttag caactgcgtt acgaaggtct tttgacattg cattctctac
     1141 catattggta gctgcttttg cctggttggg gaagaccata gcatctccct gaacattaga
     1201 tctaaatgct gttctgtgct gacgcaagag atcaaacgta acatccaaat cagttgcagg
     1261 gtttgctaat tcttcacgta ggttacgcaa ggatgtaagc aggctttgat tggcagacgt
     1321 cccaagccgt tcctgtcttg cgatcgctgt attcagagca ttcatggtat ttgtggtatc
     1381 aactgcggca ttacccattt tattggtgac gtcattgata acagcgccag cggcatcctt
     1441 ccgccccctt aacgtggtgg taagagattt caccacatca tcagggttgt actcaccaaa
     1501 acggtcaaaa taattactta ccagcttact acgcgttgca tattgctccg ctcgctttga
     1561 gcctgtcccg agcaaagccc cctcggcatc ctgagttagg ccgcgagtga aagcattttt
     1621 cggcgggata acatcagatg tcattggtgt cacgcccatc gattctgatg tggcaatttt
     1681 cttcgccact tctggcgcaa tatcaccttt tatagccgtt attccacgcc ctattccctt
     1741 tgctgctgcg gaaagaacac cctgagcggc aaggttaact ccggcatttt tagctgcatt
     1801 ttgtgcgaaa tcgcctttct gatttgcggc ctctgccagt gatccaatag ccatgcttcc
     1861 tgccgttcca actcctggaa ctaaataccc accaattgtt tcaccggctt gcgcgtaggg
     1921 gtctgtcggt ctgtctactg gacgataaac atcatccaaa accttgggtc caccaagccc
     1981 ctgactgatt gcattaatca gacttgcgcc gccctgcaat acgtcaaatg gtatgtttac
     2041 cagaccacga ccagcctgtt ctgcaatttg ccctgcactt tgaccaccag tgagccaatc
     2101 gccagcttgt tgcatcaatg atggttcttc ccgtgttggt gcattattgg cctgattaac
     2161 tgtttgttgc tgaacagcct gaccagcaaa atactcatca atggcggtgc caatatcttc
     2221 ggtgctcgta ccatcaggaa aggtaaatgt cttaccgttt gcagttactt tcatcattcc
     2281 accgtaaatt gaatgcctga ttttgaggta tatgatccaa cctgattccg tggttctcct
     2341 gaaggtgtcg aatcttgtgc tggcgctgcg tcagtattca ttgacatata ccgcttaacg
     2401 gcactcccca atgattcacc ttttttaaca tccaacccca atatctgacc gccattacgc
     2461 gattgtccag ggttgccatt cgcgctcatc cactcggctt taaactcatt aaactgcgcg
     2521 tttcgtcgct caaggtttgc cattgcatca agccatcttg cgaccgtctc agggttatcc
     2581 atgtcagttg gcgcaccctg tcgaacgatc tcaacgtctt tatccgttgc tgggccggga
     2641 ggtaggaatt taagaacctg actgttaaca agggcatttt ggcgaatgcg caaatcacgc
     2701 aatgttgtat cgcttccggt aagttttgcg aacatgttct gtgcgttacc gaacaaacct
     2761 gtcgttggtt tttctgctct gaactgttga gcaagcgcac tcatagaatt ggctgagttt
     2821 gatgatgctg tagcattgtt tacagccgtc tcgatgcctt tttccatgtt tactgacagc
     2881 ttaggtgctt cgctaatcaa ctgctgagcc ttttcctgcg cttgctgcat cttaaacccg
     2941 aactcttgct gatccagagc caagcgttgt gctgcgatat tgtgcccagt cattgctgac
     3001 tgataggaaa ggttttgccc tctcgcctga agtgcttctc cagcctgatt gctgcggatt
     3061 gtctctgcca gcctgcctcg gtcaatttca cgaccagcca tcttgtcctg aacattgaag
     3121 taatcaatcg gaccaagagc agccattcca aggtgatcaa caaactcacc aaatcctgaa
     3181 ggattctgct gatacatctg agcaacgctg ttagggccaa caccgacgcg cgccagctca
     3241 ttggcgttgt tttgcagcca tgattgcatt gcttctggag acgaggccgc aaggcgtgcg
     3301 ccagccgcta aggtgccgat agaattacgc tggtcttcat caatgaatcc catgccttta
     3361 cgaacggatt caatctggtc tggatattga gtagccaact gacgcaaagc accgcgatca
     3421 ccagacgcat aagcattagc gtacgcctgc tgaaattctt tctgccgctg agcctgattt
     3481 tcctgctgaa acacccctgc aatacctgaa aggccttgca aagcagtcag cccaacattg
     3541 ttagcgcctg aacgctcaat atcattgttc tgcctgataa gctgaagcgt attgccgatg
     3601 tcatttacgc tcggagcgtt tgagttgacg ccgccgatac cagctaacaa tccgccattt
     3661 gatccttgcc aagtagccat gattacccct taaaacaacg agccaagcaa tccgatacca
     3721 gcaccaatgc cagcgcccca aggtgttgat gttcccaaaa ggctggcaag acctgcaccg
     3781 gcaatcgcac cagatgttcc gccactaatt gcagtctgaa gacttgatgg tttattggca
     3841 ttagcagcgg caagagctgc gctttgctgt gcaatgctgc tcatgttgtt ggcgtacgtc
     3901 tgcccggcgt ttgcctgacc ttgcagcgca ccaagcccaa cgtttgccag attgttgtaa
     3961 ttgctcatct ggtttgataa ccaagactga ccgagtgtcg gcgcgatcgt agccagttga
     4021 ttgcttgtgg ctgtcgaacc aagtccaccc gtcgcctccg cagcagcaag actctggtaa
     4081 cgagcctgac ctgcaaggtc tttatactgc tgagagttgt aatactgatt aagtgcctgc
     4141 ccctgacctt ctaaactgga aaggttctga agctggttaa catactgctc cgcaagaggc
     4201 gtgaacggag taaggttttt catgatcgtc tgccactgct gattttgcag gtctgcggca
     4261 tacttctgtg aaccgccccg ggtttcctgg agagtgtttt
//
LOCUS       DAETPZ010000197         4295 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.203, whole genome shotgun sequence.
ACCESSION   DAETPZ010000197 DAETPZ010000000
VERSION     DAETPZ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.203"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            80..521
                     /gene="tnpA"
                     /locus_tag="K0W59_004148"
                     /pseudo
     CDS             80..521
                     /gene="tnpA"
                     /locus_tag="K0W59_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317271.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(639..1583)
                     /gene="hypT"
                     /locus_tag="K0W59_004149"
                     /gene_synonym="qseD"
     CDS             complement(639..1583)
                     /gene="hypT"
                     /locus_tag="K0W59_004149"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HBI3024908.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQEAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTPPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            1928..2647
                     /locus_tag="K0W59_004150"
     CDS             1928..2647
                     /locus_tag="K0W59_004150"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBI3024909.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2713..4011
                     /locus_tag="K0W59_004151"
     CDS             2713..4011
                     /locus_tag="K0W59_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HBI3024910.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT         1078 a          931 c         1057 g         1229 t
ORIGIN      
        1 ggggaaaaac gagtattttt agtcatcctg tttacctctt tctcaggaag tttagtctcc
       61 aggattcccg gggcggttca tagcgcacac ccgatggaac tgtaaatatc acatagtttt
      121 ttgcgccaaa ataccgaaga caggtgttct acagagagaa gcgtagagca acaggcagta
      181 ttttgagaaa gctgtgtgag tggaaaagcg tacggattct ggaagctgaa tgctgtgcag
      241 atcatatcca tatgcttgtg gagatcccgc ccaaaatgag cgtatccggc tttatgggat
      301 atctgaaagg gaaaagcagt ctgatgcttt acgagcagtt tggtgatttg aaattcaaat
      361 acaggaacag ggagttctgg tgcagggggg actacgtcga tacagtgggt aagaacacgg
      421 cgaagataca ggattacata aagcaccagc ttgaagagga taaaatggga gagcagttat
      481 cgattccctg tccgggcagc ccgtttacgg accgtaagta acgaagttgg atgcaaatgt
      541 cagatcgtgt gcgcctgtta gggcgcggct ggtaagagag ccttataggc gcatttgaaa
      601 aacctccggc tatgccggag gatatttatt gcacgtcgtc aattgcggcg tgctggctct
      661 tccattgata tctgctggat taaatcgtct ggcatatgtt cctgtaaaat acgccagagg
      721 ttctcggcgg ttctattcag tcgggtgttc attcggtaaa tataagcctg aatggggact
      781 gtcaatttag cagcatcaag gcatatcaga cgtttggtct gtagttcatc gacgatcgac
      841 cagatcggta accaggcaat gccgtatccg ttgagcgcca tattctttaa taactcgctc
      901 atcgaagaga taaatatcgt gcgcccggta atacctccaa cctctgccag atgacgattg
      961 accaacctgc ccatataaga tgtggcggta tagttgagca aaggaacctg gggctgagtg
     1021 atatcgaata cagggtgccc ctgtgcgtcg gcggcacata ccgggtacaa ctcagattca
     1081 aacagtttca ggcagcaaaa aggcgattgc ataagatctt catcgcgaaa cgaaataata
     1141 aagtcactct tgccttcgcg taacgtattt acggcctgga caacatcaat ggcttcaaca
     1201 tgatagacaa attctccgcc ataggcggta agtgaatgca ccagtttggg taatacgctc
     1261 agggatagtg agtgcgcggc tgcaatttta atgttgggaa cgccgagtaa actctggccg
     1321 tttaactccc ccaaattgca ttcaagctgc tgtaataagc tgcgagtctg ggaatgaaag
     1381 agcttgccct cttcggttaa ttgtaaaggg ggggtcgtgc gatcgaataa aacaacgccg
     1441 atcgctgcct ccagcgcctt aattcgtcgg ctgaatgcgg gttgcgaaag attacgctct
     1501 tttgcctcct gagaaaaatg gcgacaggct tccagtgtca ggaaatcgta taaccatttt
     1561 gtctcgatat tcttcattac catggcacct gtattttcaa tccaaagaca taataaaaca
     1621 tcccggcaat aaaaataatg ttctcaatta gcacaacctg gcctttatca ttcgttttgt
     1681 gcataacccc ttcatttccg gtattgacgt tgatatcaca tttcgccaaa aaaacgaaat
     1741 ttttccgtca gataaccaga atacatcatc actatggtga ataagcggaa tgttccatag
     1801 ctgcatcatg cgtgtcttgc atcgccttgt acaaccttgc atttctcatc gcccatttcg
     1861 actgttaaaa aagagagcag cagttattta ccgcgtattg tcattaacgt tttgagaggc
     1921 aggtattgtg aaaggtttaa ttggtgtact cggtggaatg gggccggcag ctaccgtcga
     1981 cctgtttaat aaatttgtaa cttttaccgc tgcacaatgt gatcaggaac atattccttt
     2041 aattatttct tcgatcccgg atattcctga ccgtaccgat gcattaatgc atcatgggcg
     2101 ttcaccatta cccgttatgc gtgattatat gcataagctg gaagatgccg gggcagaatg
     2161 tattgttatt ccctgtaata ccgcgcatta ctggttcaaa gaattaaaag atgcttgtca
     2221 tattgatata ttaagtatcg tagaaactac gatcaatgaa gttcgcgcct gtggtaaaac
     2281 ccgaattggc ttgttagcca ccaatgccac gctatatatg ggtttatacc aaaaaggaat
     2341 tgagtcgctg ggatttacct gtgtcagccc cgatgccgac ggtcagaaaa aagtgatgga
     2401 aagtatttat tccttgaaag ctggcaatat agcgcacgca caaaagttga tgaatgagca
     2461 ggctgaaata ttattttccc gcggtgcaga aatccttgtt cttggttgta cagaaattcc
     2521 ggttattctg gcgcaagccg ttaaagagca gccttcccgc tatattgact caacggcgtc
     2581 actcgttcgt gccggaataa aatggtacga aaatcgtgtc ggtaaacatt atcttttaac
     2641 ccaataatca tttaaatcgc agccagaaag ttattcgctt ttaactgaat tatatttata
     2701 acggagaaca ttatggtttg gctggaaatt atcgtagtac ttggtgcaat attttttggt
     2761 attcgccagg ggggaatcgg tattggttta tgtggcgggc ttgggcttgc cattctgact
     2821 ctgggatttg gtctgcctat ggggtcgccg ccagtcgacg tgatcctgat tattatgacc
     2881 gttgttgtgg cggcctcagc cttacaggcc gcaggcggaa tggattatct ggtacgcgtc
     2941 gctggtaatt ttatgcggcg taacccgaaa tatatcaaca taatcgcgcc gattatcacc
     3001 tggtctatga ccattatggc cggtactggg tttattgttt tttccacgct gccggtgatt
     3061 gctgaagtgg cgaaagagtc cggtatccgt ccttcccgca cgcttgccgg ttcggttgtc
     3121 gcttcgcagg ttgcgatttc cggttcgccg atcagcgccg caatggcggc tatgctcact
     3181 attatggagg taaacggtgt cagttttatt caagtgatgt cggtatgtct tcccacctct
     3241 tttgctgccg cgatggtcgc cgcttttatt gcttcccgac agggatgtga gttgcaggat
     3301 gacgaggtct accttgagcg cctgcaaaaa ggactggtgc aaaaatacga gaataatagc
     3361 agcatcaagc caggtgcgac gctctccgtc ggattgttcc tgctggcgac ggtcgctatt
     3421 gttattcttg ctgccttccc ccaactgcgt ccgggttttg atatcggtaa accgatggaa
     3481 acacgcgata tcattattat ctgtatgctc tctgccgcct gcctgatggt ggtgctgtgt
     3541 aagacggcga ccgatgccat tattctcacc tccacattcc gtgcggggat gagttcgctg
     3601 gcggtaattc tcggcattgt aaccttaggc accactttta ttgatgcgca tttaactgag
     3661 attaaagata ttgctggtga tattttgcag gcgtatccga tgttgttggc gctggttctt
     3721 tttggcactt gcgcgctgct ctactcgcaa ggtgcgacca cgccgttaat tatcccgctt
     3781 gccgtcgcgc ttgacgtgcc cacctgggca attctggcgt cctatgtggc cgtcaccgga
     3841 gtatttgtac tgccaacgta tccaacatca ctggctgcca tggaatttga tactaccggc
     3901 acaactcgtg taggcaaata tgtgctgaat catccattta tgctgcctgg gttgggggga
     3961 attattgctg gagtggcatt aggctttgtg attgcgccaa tgattattta gcactatcaa
     4021 acatcagtat cgatggttta tacacaataa agacagatat ggcggaatat agtctgttgt
     4081 aagcgccaac aaagaatagg ggaattccgc catgtctgaa catcttcaca atatggtatt
     4141 aagtgttgtc gtttaatgat ttatttcttg gttatgttgt gtattagctg catctatcgc
     4201 tttttgatac ctgaggtgta ctggcaatag cggacactac catttgttct ttttttaagc
     4261 agtcatctga tgatattttt ccctgaaggc tgccg
//
LOCUS       DAETPZ010000198         4263 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.204, whole genome shotgun sequence.
ACCESSION   DAETPZ010000198 DAETPZ010000000
VERSION     DAETPZ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.204"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..2057
                     /locus_tag="K0W59_004152"
     CDS             <1..2057
                     /locus_tag="K0W59_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI3024911.1"
                     /translation="SMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQ
                     TRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQVPRLEGAWAPEH
                     SVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTH
                     LNEDRRVLNSMIHDAREKAGELGKVQVMVPVLNTANIRDGELRRLSTWENNPDALALV
                     DNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTIRVGTGDRIRFT
                     KSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAIIAHGAQ
                     GASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKG
                     TAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKY
                     PQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNG
                     ESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIPDNSVQPGAG
                     NGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTEQAVREIAGQER
                     DRAAITEREASLPESVLREPQRVREAVREVARENLLQERLQQMERDMVRDLQKEKTPG
                     GD"
     gene            2077..2823
                     /gene="traX"
                     /locus_tag="K0W59_004153"
     CDS             2077..2823
                     /gene="traX"
                     /locus_tag="K0W59_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HBI3024912.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            2878..3438
                     /locus_tag="K0W59_004154"
     CDS             2878..3438
                     /locus_tag="K0W59_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HBI3024913.1"
                     /translation="MIEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            3569..3781
                     /locus_tag="K0W59_004155"
     CDS             3569..3781
                     /locus_tag="K0W59_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HBI3024914.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            4025..>4263
                     /locus_tag="K0W59_004156"
     CDS             4025..>4263
                     /locus_tag="K0W59_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HBI3024915.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT          994 a         1042 c         1389 g          838 t
ORIGIN      
        1 gctctatggt gggcaatacc gacatggcac gggcatacgc cctgattgcg gccggtggcg
       61 gtcgggctgt ggccagcggt gacacggacc agctgcaggc catcgcgccc ggtcagcctt
      121 tccgtctcca gcagacgcgc agtgctgccg atgtggtcat catgaaggag attgtgcgtc
      181 agacgccgga actgcgggag gcggtataca gcctgattaa ccgggatgtg gaaagggcgc
      241 tgtccgggct tgagcgtgtg aaaccgtctc aggtgccacg tctggagggc gcatgggcac
      301 cggagcactc cgtgacggag ttcagtcaca gccaggaagc gaaactggca gaagcgcagc
      361 agaaggcgat gctgaaaggc gaggcttttc cggatgtccc catgacactg tatgaagcca
      421 ttgtccgcga ctataccggc aggacaccgg aagcacggga gcagacgctg attgtcacgc
      481 acctgaatga ggaccggcgc gtactgaaca gcatgattca tgatgcacgg gaaaaggccg
      541 gagaactggg gaaggtgcag gtcatggtgc ctgtcctgaa cacagcgaat atacgcgacg
      601 gggagctgcg tcgtctctcc acctgggaga ataacccgga tgcccttgcc ctggtggata
      661 atgtgtatca ccggattgcc ggtatcagca aggatgacgg gctgataacc ctgcaggatg
      721 cggaaggtaa cacgcggctg atttcgcccc gggaggcggt ggctgaaggc gtcacgctgt
      781 acaccccgga caccatccgg gtggggaccg gtgacaggat acgcttcacg aagagtgacc
      841 gggagcgcgg ttatgtggcc aacagcgtct ggacggtgac agcggtttcc ggtgacagtg
      901 tcacactttc tgacggtcag cagacccgtg tgattcgccc cggtcaggag cgggcagagc
      961 agcatattga cctggcctat gccatcatcg cccacggtgc acagggggcg agtgaaacct
     1021 ttgccatcgc gcttgaaggc acggaaggta accggaaact gatggccggc tttgagtcag
     1081 cctacgtggc cctgtcgcgt atgaagcagc atgtgcaggt gtacaccgat aaccgtcagg
     1141 gctggacgga tgccatcaac aatgccgtac agaaaggaac tgcccacgat gtgtttgagc
     1201 cgaaaccgga ccgggaggtc atgaatgcag agcggctgtt cagtacggcg cgggagctgc
     1261 gggacgtggc ggcaggccgt gccgttctcc gtcaggcggg gctggccggg ggagacagtc
     1321 ctgcacggtt tattgctccg ggacgtaaat atccgcaacc gtatgtggca ctgccggcgt
     1381 ttgaccgtaa cggcaagtcc gccggtatct ggctgaaccc gctgaccacg gatgacggaa
     1441 acgggctgcg gggattcagc ggtgaaggtc gggtaaaagg cagcggggat gcgcagttcg
     1501 tggccctgca gggcagccgt aacggagaaa gcctgctggc tgataatatg caggatggcg
     1561 tccggattgc ccgtgataat cctgacagtg gtgtggtggt aagaatcgcc ggtgaaggcc
     1621 gtccgtggaa tcccggtgcc atcaccggtg gccgcgtgtg gggggatatc ccggacaaca
     1681 gcgtccagcc gggagccgga aatggcgaac cggtcacggc agaggtactg gcacagcggc
     1741 aggctgaaga ggccatccgc cgtgaaacgg aacgtcgcgc agatgaaatt gtccgtaaaa
     1801 tggcagagaa caaacctgac ctgccggacg gcaaaacaga gcaggctgtc agggaaattg
     1861 ccggtcagga gcgtgaccgg gctgccataa ctgaacggga agcctcgctg ccggagagtg
     1921 tactgcgtga accacaacgg gtgcgggaag cggtccggga agttgcccgg gaaaatttgc
     1981 tgcaggagcg tctgcagcag atggagcggg atatggttcg tgacctgcag aaagagaaaa
     2041 ccccgggcgg agactgatac aggaagacaa acactgatga caacggataa cacgaacacg
     2101 acacgtaacg attcactggc tgcacggacc gatacctggt tgcagtcatt tctggtctgg
     2161 tcacccggac agcgggatat catcaaaacg gtggcactgg tgctgatggt actggaccac
     2221 atcaatctga tattccagct gaagcaggaa tggatgtttc tggccgggcg aggagccttt
     2281 ccgctttttg ccctggtgtg ggggctgaat ctgtcccgtc atgcgcatat ccggcaaccg
     2341 gccattaacc ggctgtgggg atggggaatt attgcccagt tcgcatatta tctggcaggc
     2401 tttccttggt atgaggggaa tatcctgttt gcctttgcag tggcggcaca ggtgctgacg
     2461 tggtgtgaaa cgcgcagtgg gtggcgtaca gcagcggcca tactcctgat ggccctgtgg
     2521 gggcctttgt ccggcaccag ttacggtatt gccgggttgc tgatgctggc agtcagcaac
     2581 cggctgtacc gggcggaaga cagagcggaa cgtctggcgc tggtcgcctg cctgctggct
     2641 gttattccgg cgcttaacct tgccaccagt gatgcagcgg cggtggccgg tcttgtgatg
     2701 acggtgctga ccgttggtct ggtgttgtgt gccggaaaat cattaccccg tttctggccc
     2761 ggagactttt tcccgacgtt ctatgcctgt catctggctg tgctgggcgt tctggcgctg
     2821 tgacgggtgt ggtatctttg gccgcaagag gatgattcgt cagaggcaga acacagcatg
     2881 atagagcaga agcgaccggt actgacactg aagcggaaga cagagggaac agcgcctgtc
     2941 cgcagccgga aaaccatcat caatgtcacc acgccaccaa agtggaaggt gaaaaagcag
     3001 aagctggccg agaaagcggc ccgtgaagca gagctggcgg caaaaaaagc gcaggccaga
     3061 caggcgctgt ccatttatct gaacctgccc acgctggatg aggctgtgaa taccctgaag
     3121 ccctggtggc ctggattatt tgacggtgac acgccccggc ttctggcctg cggtatccgg
     3181 gacgtgttac tggaagacgt ggcgcagcgg aatatcccgc tctcgcataa aaaactgcgc
     3241 agggcgctga aggccatcac ccgttcagaa agctatctgt gtgccatgaa agccggtgcc
     3301 tgccggtatg acacggaagg gtatgtgacg gagcatattt ctcaggagga ggaagcgtat
     3361 gcgggagcgc gtctggcgaa aattcgtcgc cagaaccgga taaaggcaga acttcaggcc
     3421 gtgcttgatg agaaataaaa aaagcctccc ccagagagag gaggcaggga aataaggctt
     3481 aaaaggaaat aacttccgct tccgaaagga agccagtcca ctgaattatt tatcacaata
     3541 atactgacaa ctaaaggggg acaagaaaat gaatggattc agaaacagtt cacggaacgg
     3601 ccaggtctgg cgttaccagc gtgccggcgg gcgggccgtt attctggaag tcagtggacg
     3661 ctggatggaa gcggcagaag catggcgacg ggctgcctgt gttgcgccta ggacagactg
     3721 gcagcagttt gcccggaaaa gggctgaaca ctgccaccgg cgctgccggg gaagggtgta
     3781 acgaaaaaag cagccttcat tactctagga gtcagaaggc tgaaaggagt ttatgagatt
     3841 tgcttcttaa tatccggtcg ggcagtatga ccggacagtt ctgagtagca cagagtcttt
     3901 tccgcattaa gtcgattttt tatattttgt gattaatttc acaaaataag gtgttattca
     3961 atgtgtgctg cactattcag ggggtctgaa atacaggtgg catttatctg atatggagaa
     4021 taacatgagg aaatatattc cactggtatt atttatcttt tcatggccgg tattgtctgc
     4081 agatattcat ggacgggttg ttcgtgtact ggacggtgat accatcgagg tcatggactc
     4141 actgaaggcg gtgcggatca ggctggtaaa tattgatgca ccggaaaaga aacaggatta
     4201 tggccggtgg tcaacagaca tgatgaaatc actggtcgca ggaaaaacgg ttaccgtcac
     4261 ata
//
LOCUS       DAETPZ010000199         4129 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.205, whole genome shotgun sequence.
ACCESSION   DAETPZ010000199 DAETPZ010000000
VERSION     DAETPZ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.205"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..158
                     /locus_tag="K0W59_004157"
     CDS             <1..158
                     /locus_tag="K0W59_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024916.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
     gene            complement(167..565)
                     /gene="vapC"
                     /locus_tag="K0W59_004158"
     CDS             complement(167..565)
                     /gene="vapC"
                     /locus_tag="K0W59_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HBI3024917.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(565..792)
                     /gene="vapB"
                     /locus_tag="K0W59_004159"
     CDS             complement(565..792)
                     /gene="vapB"
                     /locus_tag="K0W59_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HBI3024918.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            874..1293
                     /locus_tag="K0W59_004160"
     CDS             874..1293
                     /locus_tag="K0W59_004160"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HBI3024919.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRHHPCGGTGLHTGDDGRTVRNGADR"
     gene            1280..4099
                     /gene="traI"
                     /locus_tag="K0W59_004161"
                     /pseudo
     CDS             1280..4099
                     /gene="traI"
                     /locus_tag="K0W59_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
BASE COUNT          930 a         1060 c         1293 g          846 t
ORIGIN      
        1 tcagtgaatc cggtgaagtg gtggatatgg ccgcttatga ggcatggcag caggaaaatc
       61 atccggacac ctggcagcag atgcagcgtc gtgaagaggt gaacatcaat gtgcaccggg
      121 aacgcgggga ggatgttgag ccgggagatg atttctgatg aacaggtcag ctccagtctt
      181 cagttctcag gccgcccaca cgttcaaatt cccgggtgtt attagtcaca ataatcagcc
      241 cccgactgcg ggcatgacct gcgatcattt gatcaaatgg cccgacaggg cgtccctgac
      301 gggcaagttc tgctcttatc tggccggtgt gtgtggctgc agcagcgtcg taatccagaa
      361 catcaatgcg ggaaacaaat ccctcgatca cagcgagatt acgttcaggc atctggcttt
      421 tttctgcacc atatatcagc tccatcaggg tgaccgaact gatacacatt ttcccctggt
      481 tcaggttaaa acgctccctg acgctggcgg gtttgttctt tatcgtaaaa atgcagatgt
      541 tggtatcgag cataaacttc agcatcagaa tgactccctt tcctgcatgc cgggctgttc
      601 cctgttatcc ataaaatcgg tgctgacgct gtgtccgtcg aaccattcgt cccatgtctc
      661 tccggctggc gtaatgattc tggttcgtcc gacagcaatc acttcaacgc gttttacgtt
      721 ttccggcaat gcaaccgctt ttggcagtct gaccgcctgg ctgcggttgc tgagaaatac
      781 ggtggtttcc attatttgcc tccttatgtg gatatgtctt gtgtatatct attgtgggat
      841 tgataccggg atatgtcaaa gggatatacg tttatgatga gtatcgcgca ggtcagatcg
      901 gccggaagtg ccggtaatta ttataccgac aaggataatt actatgtgct gggcagcatg
      961 ggagaacgct gggccggcca gggggctgaa caactggggc tgcagggcag tgtcgataag
     1021 gatgtcttta cccgccttct ggagggcagg ctgccggacg gggcggattt aagccgcatg
     1081 caggatggca gtaacaggca tcgtcccggc tacgacctga ccttctccgc tcccaaaagt
     1141 atctccatga tggccatgct cgggggggac aaacgtctta ttgaagcaca taaccaggcc
     1201 gtggattttg ctgtccgtca tcacccgtgt ggaggcactg gcctccacac gggtgatgac
     1261 ggacggacag tcagaaacgg tgctgaccgg taatctggtg atggcactgt ttaaccacga
     1321 caccagtcgc gatcaggaac cacagttaca cacgcatgcg gtggtgacta atgttacgca
     1381 gtataacggc gaatggaaga cactgagcag tgacaaagtg gggaaaacgg ggttcattga
     1441 gaatgtgtac gctaatcaga ttgcctttgg caggctctac cgggaaaaac tgaaagagca
     1501 ggttgaggcg ctgggctatg aaactgaagt ggtcggtaag cacggtatgt gggaaatgcc
     1561 gggtgtaccg gtggaggcct tttccggacg ctcacagact atccgggagg ccgtcgggga
     1621 agacgcctcg ctgaaatccc gggatgtggc ggcgctggat acgcgtaaat ccaaacagca
     1681 cgtcgatcct gaagtcagaa tggccgagtg gatgcagacg ctgaaggaaa ccgggttcga
     1741 catccgggca tatcgtgacg cagcggaaca acgggcatat acccgcacgc agacacccgg
     1801 acccgcttca caggacgggc cggatgtgca gcaggcggtg acacaggcca ttgccggatt
     1861 aagtgaacgc aaagtgcagt ttatgtatac ggacctgctg gccaggacgg tcggcatact
     1921 gccgccggaa aatggtgtga ttgaacgggc acgcgccggt atcgatgagg ccatcagccg
     1981 tgagcagctt atccccctcg atcgtgagaa ggggctgttc acgtccggga ttcatatgct
     2041 cgatgagctg tcagtccggg cactcagtcg tgacatcatg aaacagaacc gggtgaccgt
     2101 acatccggag aaaagtgtcc cccggacggc cggttacagc gatgccgtca gtgttctggc
     2161 acaggaccgt ccgtcgctgg ccattgtgtc cgggcagggc ggtgcagccg ggcagcgtga
     2221 gcgggtggct gaactggtca tgatggcccg ggagcagggg cgggaggtgc agattatcgc
     2281 tgctgaccgt cgctcgcaga tgaacctgaa gcaggatgaa cggctgtccg gtgaactgat
     2341 aaccggacgt cgtcagttgc tggaaggcat ggccttcacg ccgggcagta ccgttatcgt
     2401 cgaccagggc gaaaaactct ccctgaaaga gacgttaacc ctgctggatg gtgccgcacg
     2461 tcataacgta caggtcctga taaccgacag cgggcagcga accggtacag gcagtgcact
     2521 gatggccatg aaggatgccg gggtgaacac atatcgctgg caggggggag aacagcgacc
     2581 ggccaccatc atcagtgaac cggaccgtaa tgtccgctat gcccggctgg ccggagattt
     2641 tgcggccagc gtgaaagccg gagaagagag cgtggcacag gtcagcggag tacgggaaca
     2701 ggccatactg acacaggcca ttcgcagtga gctgaaaaca cagggcgtgc tcggacaccc
     2761 ggaggtaacc atgaccgccc tgtcaccggt ctggctggac agccggagcc gttatctgcg
     2821 ggatatgtac cgcccgggaa tggtgatgga gctgtggaac ccggagacac gcagtcatga
     2881 ccgctatgtg acagaacgtg tgacggcgca gagtcacagc ctgaccctgc ggaatgcgca
     2941 gggcgaaacg caagtggtgc gtatttcttc cctggacagc agctggtcgc tgttccggcc
     3001 ggaaaaaatg ccggtggcag acggtgagcg tctgagggtg acagggaaaa ttcccggact
     3061 ccgcgtctcc ggcggtgacc gcctgcaggt ggcatccgtc agtgaagatg cgatgacggt
     3121 tgttgtgccg ggacgggctg aaccggccac cctgcctgtg agcgattcac cgttcacggc
     3181 actgaagctg gagaacggct gggtggaaac gcccgggcat tccgtcagcg acagtgcgac
     3241 ggtttttgcc tccgtcacac agatggcaat ggacaatgcc accctgaatg gtctggcccg
     3301 cagtggtcgt gatgtccggc tgtattcctc actggatgaa acccgtactg cggaaaaact
     3361 tgcccgccat ccctccttta cggtggtttc tgagcagata aaggcgcgtg ccggtgagac
     3421 attgctggaa accgctatca gtctgcagaa agccgggctt cacacgccgg cacagcaggc
     3481 cattcatctg gcccttcctg tgctggaaag taaaaacctg gccttcagca tggtggacct
     3541 gctgacagag gcgaagtcgt ttgctgcaga aggaaccggt tttgctgacc tgggagggga
     3601 aatcaatgcg cagataaaac ggggtgattt actgtatgtg gatgtggcaa aaggctatgg
     3661 cacaggcctg ctggtttccc gtgcgtcgta tgaggcagaa aagagcattc ttcgccatat
     3721 tctcgaaggt aaggaggcgg tcacgccgct gatggagaga gtacctggcg aactcatgga
     3781 gaaactgaca tcaggacagc gcgccgccac ccgtatgata ctggaaacgt ctgaccgttt
     3841 cacggtggtg cagggctatg ccggtgtggg taagaccaca cagttccggg cggtgatgtc
     3901 agccgtgaac atgctgccgg agagtgaacg tccccgtgtc gtggggctgg ggcccacaca
     3961 ccgtgcggtc ggggagatgc gcagcgccgg cgtggatgca cagacactgg cgtcctttct
     4021 gcatgacacg cagcagcgca gcggagaaac gccggatttc agcaacacgc tgttccttct
     4081 cgatgagagc tctggtaatg ctgccaactt actgatttag tgtatgatg
//
LOCUS       DAETPZ010000200         4114 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.206, whole genome shotgun sequence.
ACCESSION   DAETPZ010000200 DAETPZ010000000
VERSION     DAETPZ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.206"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            227..1200
                     /locus_tag="K0W59_004162"
                     /pseudo
     CDS             227..1200
                     /locus_tag="K0W59_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1197..2102
                     /locus_tag="K0W59_004163"
     CDS             1197..2102
                     /locus_tag="K0W59_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HBI3024920.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASVAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLILADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            2099..3169
                     /locus_tag="K0W59_004164"
     CDS             2099..3169
                     /locus_tag="K0W59_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBI3024921.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECDTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISILNGFDNLMAQPLLSDEPLTALLDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGDNSGGLLSFTLAHTDAWRQQGYEDTMLTMEHIRKPLAARQVLSRSETVL
                     QKSLEITEEADLALRNAMARIK"
     gene            3305..3982
                     /locus_tag="K0W59_004165"
     CDS             3305..3982
                     /locus_tag="K0W59_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024922.1"
                     /translation="MQLPVKLLMSLISLVSVIARAGKYKNYIRDEIKYWRYTSYKGGK
                     FPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNVEAASVKQALSCDPNARA
                     EQPGACPTTYELYEGDAAYKAALDKALKPVGLSGMFGKGGYMDGPGGNVTPVTINGTV
                     WLQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYLVK
                     NYVAAVN"
BASE COUNT         1070 a          970 c         1139 g          935 t
ORIGIN      
        1 ctcacttatc gatcaactga taacggaagt catactgagt tcacactcca gagctgcggc
       61 actggcggtt gattctgccg ccgcaaaact gatgcagaat gcagacaata tcagtgaata
      121 cctgtcgtta caccatcagg ggctgatgca aagtattcag tcactgcagg cgcatatcac
      181 cagcctgctt gaggatatcc gggaaatagc agactgtcag gagcaggtga ccgctgatgt
      241 cagaatggcc atggaggaga tcgatgccag aacccgggaa ttactgacgg ggtctgcacc
      301 tcactggaag aggagctgaa tgactatttc agaagcggta aacgcaaaga acagcaaatg
      361 ctggaggaag aaaattcagc gcagccccgg gagagaaacg cattcgcttt ctttcacgat
      421 attttcggaa ccggaaatca gcatgacagg atgcgggact ttgatccgga cagccccgaa
      481 ataaaattca gtgatcgcag ggaagccctt gaactgatga cgcaaatcga atcgaccgtg
      541 accagcctgc accgagaggc tgaagcacag ttccggcctg agctggagaa aatcgtcagc
      601 gggattgaaa caggttttcg tggcacggcc ctgtacgcca cagaaaacat tgccggtcgc
      661 atcaataccc gcctggagga tgagggcttt accgtaaaaa tcagttttcc ggcagtcagt
      721 cagttacaaa cccggctcgc ggtaaaaaca aatctgagtg cgcttatgga ggaaagaacg
      781 gagaccgtca cccgtcgccg tcggcagcgt ggtttatggg gaaagatttg tggagcgttt
      841 ggcaccagtg actggggctg ggaaacctat aaagaggatg tgagccgcag tgttatcaat
      901 atcaacaccg tcaggaagga agttatgtca ctgacccggg catatttcgg ggagctgcag
      961 gcatccattg agcaggatat taaccagccc gtccgccagg aaatcgatgc ctttttctgc
     1021 gcattcaggg agaaagttga acaactgcgt aacacgctga ttcagagctc tgaagatcat
     1081 aaacgcgatc agcaggcaca ggaacggctt accaggcgac ttcaggcatt aaatgaaagg
     1141 gttcctgagc tcattactga cagtaaggcg ctgagggaag agctggagac aatgctgtga
     1201 cttcaccatt tattcaacaa attgctgata accgggtatg tcaggtactc acctgtcttc
     1261 ctgaaaaatt tgtggttgat ttcgcaaacg gaatcgatgt tgcacaggag catatccgca
     1321 cggccggaga gcgcacgttc ttccggcgtt taaaagaggg actgaccggc gaaggcgctg
     1381 cacggcagaa tgccatcaat gcttcggttg cacagggcgt tgaggcgtcc ctgcgctggc
     1441 tgacggagct gacaacgtca cttgccacca caaactacgc gattacccgg gtaaacgaca
     1501 gggtcagttc actggtcagt gatactgcca ggttggcgca ttattccgca gatacacggg
     1561 agcagttact catcctggcc gatcaggttc accataaact gaatcatctt gaagaaaaac
     1621 tccaccgtgt tgaccaggtt cagcgggcgc agttacatct tgagcagata ttctcatggt
     1681 ggagcgccgg gcgatacgcg tcattttccc ctgccggacg ttgttatgtg gcacttgaag
     1741 agcttcgctg gggcgcgttt ggtgatgtga tacgtcaggg cgaaacaggc caggttaacc
     1801 agctactgga tattctcaga cataaagcat taacgcagat ggcacaggag agtggcggta
     1861 gtgcaaccgt gcgtctgaac actctggact ggcttggtgg tcagggccgg gagcaggcag
     1921 ataacgagtg gcatgacgcg attaactggc tgggagactg gtgcagtgaa gagcagcatc
     1981 cggtgatctg gtcaaccacg caggctgcag aacacttacc ggttcgcatg cctcgcctct
     2041 gctctgcaga acgcctctct gaaagtatgg tcgatgaaat atttcagaag ggggctgcat
     2101 gagtacagaa atgaaaacgg ggctggtgct gtccggtggc ggggcggtgg gcgcttatca
     2161 ggcgggagtg gttaaggcac tggcagagtg tgatacacag atcagcatgg tttcaggggc
     2221 cagcattggc gcattcaatg gtgccattat cgcggcctct cccgatctgt cagaagctgc
     2281 cgtacgcctg gaagcgctct gggatcatct ggggaataat caggtgctgt cggtaaaccg
     2341 attggtttac ttttcattgc tgaaaaaatt gttccaggca atgaacctct gccagatccc
     2401 cggacgtgca ggagcactgc ttacgacgct tcttcgccat atatcgatac tcaacgggtt
     2461 tgacaatctg atggctcagc cgttgttgtc agatgagccc ctgacagcgc tgttggatca
     2521 ttatcttgat actgatgctc tggcagacgg gctaccgctg tatgtgtcgc tgtaccccac
     2581 agaagggggc atgcaggata ttattgactg cattcgtgct gaactgggtg ccggaaccac
     2641 gaaaaacgcc gtttttcagc atatccagag cctgccccgc ggacagcaga aagaggctct
     2701 gcttgcgtca gccgcgctgc ccctgctgtt ccgtccccgt gaggttcagg ggacaatgtt
     2761 cggtgatggt ggtatgggag gatggcgaaa tatgcaggga aatacccctg tgacgcctct
     2821 ggtcgatgcc ggatgcaata tggtgattgt gacgcatctg agtgacggtt ctttatggga
     2881 tcgccaggct tttccggaca ccacaatcct tgagatccgt ccccggaaaa ggctgaaata
     2941 tgcaggtgat ggcgacaaca gcggcggtct gctcagtttc acgttggcac ataccgacgc
     3001 ctggcgtcag cagggctatg aagacacgat gctgacgatg gagcatatcc ggaaaccgct
     3061 ggcagcacgt caggtactgt cccggtcaga aacggtgttg cagaaaagtc tggagataac
     3121 ggaagaggca gatttggcac tgagaaacgc gatggcccgg attaaataaa gacgctccaa
     3181 agaaacgcca ccgcctgtgg tggtgttttt cctagctggt gttattgaaa atattttcag
     3241 atatatatta cctgagagaa agttttaccc ctcacctgtc aattcactca ctggaaattt
     3301 aattatgcaa ttacctgtaa agttattaat gagccttata tctctggtca gcgttattgc
     3361 acgtgccggg aaatataaaa attatatccg ggatgaaata aaatactggc gatatacatc
     3421 atacaagggt gggaaatttc cggaaggttt cactgatgag aaattttcca gcgccattta
     3481 caacggaaga atatttacaa tgaaacgttt acataccctg atgttatttc tggcggttct
     3541 gtttactggc tttaacgtgg aagcagcgag cgtgaaacaa gcgctcagct gcgacccaaa
     3601 cgcccgggct gaacaacctg gagcgtgtcc aacaacgtac gagttgtacg aaggtgacgc
     3661 tgcctacaaa gctgcgcttg acaaagcatt aaaaccggtc ggactgagcg gcatgttcgg
     3721 taaaggcggg tatatggatg gccctggcgg aaacgtaacg ccagtaacca ttaacggtac
     3781 agtctggctc cagggcgacg gttgcaaagc caatacctgc ggctgggact ttatcgtaac
     3841 actctataac ccaaaaaccc atgaagtcgt tggctaccgc tactttggtt tagatgaccc
     3901 ggcctacctg gtttggttcg gcgaaattgg cgtgcatgaa ttcgcgtatc tggtgaaaaa
     3961 ctacgtagct gcggttaact aatttaagga gtctgtcatg aaaactttta tcaaaacttt
     4021 actcgttgct gtaactattc tgttctctgt cttcgctacg gcgaaacaag taaaactgcc
     4081 aaacaacatc aaatacgtta atactacaga ggcg
//
LOCUS       DAETPZ010000201         4061 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.207, whole genome shotgun sequence.
ACCESSION   DAETPZ010000201 DAETPZ010000000
VERSION     DAETPZ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.207"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(315..1172)
                     /gene="sitD"
                     /locus_tag="K0W59_004166"
     CDS             complement(315..1172)
                     /gene="sitD"
                     /locus_tag="K0W59_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HBI3024923.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1169..2026)
                     /gene="sitC"
                     /locus_tag="K0W59_004167"
     CDS             complement(1169..2026)
                     /gene="sitC"
                     /locus_tag="K0W59_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HBI3024924.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     THKALEDRS"
     gene            complement(2023..2850)
                     /gene="sitB"
                     /locus_tag="K0W59_004168"
     CDS             complement(2023..2850)
                     /gene="sitB"
                     /locus_tag="K0W59_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HBI3024925.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GNVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2850..3764)
                     /gene="sitA"
                     /locus_tag="K0W59_004169"
     CDS             complement(2850..3764)
                     /gene="sitA"
                     /locus_tag="K0W59_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HBI3024926.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         1101 a          995 c          990 g          975 t
ORIGIN      
        1 gtgcaggcgc aggtcgatcg caacgatgag atttatcgcc gtcagcggga gctgaaggaa
       61 gagctgaata gtctgaagga tttgaattcg gttaggaatt ttatcgtgga ataacagaag
      121 ctgcggcacg tcgtatgcag gaacgtgccg cggttggctg gtaaactttc gatagtgcga
      181 gtattgaatg atttctaggc gttatcgatt ttacgtattt tttgcatgat agaattcgta
      241 cctcctccca acgaccttcc atggctttcc ggacttctgt aggctggcgg acgatgctgt
      301 cctcggatgt tgtgctaatt gggtgataac agatcctgtg tatgggcgtt tccctttgtg
      361 cgtgcgttga tgctggtaac ggtaaacgtc atgatgaaca cgaccgcgaa taagacgaca
      421 atcgtcggag ctggtgcgct gtcgataaaa aaggacagcc agacaccagt cattgacact
      481 attaccgata tgccagtcgc cagtaggagc gcaatatgaa agcgttgtgt caggagtact
      541 gcaatggcgc caggcgcaat gagcagagaa atagagagaa tgatacctac tgctttcagc
      601 gtcgccacaa tggtcaggga aaccatgcac agcagcccat aatgcagcca tctcgtatgc
      661 aaaccgctta cctgcgcctg ttgataatca aagctgaaca gcaaaaaatc ccgccatttt
      721 acgctaataa caagtgtaac cagcccggca ataatcaccg tctggattat atcgccgatg
      781 gttatcccga gcatgtcgcc gaaaagaata tggtcaagat gtacgtctgg tttaattgca
      841 atatacaaga tcagacctgc ggcaaacatg ccagaaaaaa caatccccat aactgtatct
      901 tgcttgattc ggctgttgtc tttcaggtat cccgtcgcca ccgcgcagaa tactccggcg
      961 acgaatgccc ctgtagccag aggtaacccc agaatccagg taagcacgat gccaggaaaa
     1021 accgcatgac tcatcgcgtc acccatcagt gcccaacctt tcaggaccag aaagaccgac
     1081 agcagcgcac agggtatgga aactaccagt gaaatcagca gtgcgtgact cataaaggtg
     1141 aactgcagtg gctccagtaa taatgccatc atgagcgatc ctccagcgct ttatgcgtac
     1201 gccgacgatt tgccaaaagt ccatgggtgg gcgcaaatac aaatgctaaa agaaacagca
     1261 gtgtctgcgc caccacgatg attccacctg tcgcgccgtc cagaaagtaa ctgacccacg
     1321 cgccgaggaa actggttaca ctgccaatag ttacagcaat tatgagcagg cggggaaaac
     1381 gatcggttaa caaccatgct gttgcaccgg gagtcaccac cagacagatc accagaaagg
     1441 caccaaccgt ttgcagcgcc gcaacggtcg aaaccgacag cagggtgaag aagaggattt
     1501 ttaatctgcc cggatgtaat ccaatagcac gggcgtgatt ctcgtcgaaa aaggtcacca
     1561 taagatcttt ccatttgaaa aaaagcacga tgatcgagag aatgccaatg atagtgagtt
     1621 gcaggatatc cgccggatcg attgccagaa tattgcccag gacgatggtc tgaatgttca
     1681 cggatgtcgg gttcaatgac accataaaca gccccaggcc aaaaaaggac gagaagatca
     1741 ggccgatgat ggcatcttct tttaagcggg tgcgttggtt aagaaacagc atgctgcccg
     1801 ctgccagtcc gccggaaaag aaggctccca gagaaaacgg aagccccagc atatatgccc
     1861 ccgcaacacc cgggacaata gagtgcgaca gcgcatcgcc aatcagcgac cagcctttta
     1921 gcatcagata gcatgacagg aaagcgcaga gaccgccgac cattgccgag acccacatcg
     1981 cattgagcat atattcatag ctgaagggtt ccagtagcac gttcatcttg cttccctctg
     2041 caccgctgac ggtcgatgtg ccacaaaagg gcgttcatcg tcagtaataa tgctttcttc
     2101 cgagccgtta agtgtgacat ggcgcagtac gccgctaaaa gccagctcca ggttctcggc
     2161 tgtaaaagtg gtgtctgtcg gcccgcttgc caggactgtg cctttgacca tgaccgtata
     2221 atcgcaaaat gtcgtgactg agccgagatt gtgcgttgag acaagcatcg ttttgccttc
     2281 cgcgcgtaac tccctcaaca ggctgatgat tttcgcttcg gttttcacat caacgccggt
     2341 aaaaggttca tccagcagga tcacattacc ctgctgtgca atcgctctcg ccagaaagac
     2401 gcgtttcttt tgtccaccgg aaagctcgcc gatttgacga tggcgaaaat ccaccatatc
     2461 gacacgttcc agcgcatcgg tgacaatctg acgatccctc tttttggcta tacgcaacat
     2521 acccatatgc ccatagcggc ccatcatcac cacatcttcc accagtacag gaaatgacca
     2581 gtcaacctct tctgactgag gaacgtaggc aaccaggttt ttctgtagcg cctgtcgcgt
     2641 gggaataccc agaacagata tctttccgct ggtcagacgc acaaatccca taatcgcttt
     2701 aaacaacgtc gattttccgg aaccgtttac cccaaccaga gcggcaatag atccacttgg
     2761 tactgtgaag gatgcatcgc gcagtgctgt gtgcccgtta cgccaggtga cagtaacatc
     2821 attaacgaca atgcctgcag attgcatcat tatttctccc ttttcccggc tttaattccc
     2881 tgtaccagcg tactggtagt aaccttaaga aggtcgatat acgtaggtac cgggccgttt
     2941 tctgtgctca gggaatcgac atagagcaca ccgccgtagt gtgcgccggt ttcacgcgca
     3001 acctgacgcg ctggtttatc ggaaatcgta ctctcgctaa agactgccgg gatatgattt
     3061 ttcttaacta tatcaacaac cttacgtacc tgctgcggtg ttccttgttg atcggcatta
     3121 atcggccaca gataaagctc ttttagcccc aaatcgcgtg cgagataaga aaaagcccct
     3181 tcactggtga ccatccatcg ctgattctca gggagttccg taatctgctt acgcaggggg
     3241 gcaagggttt gggtaatctt ggctttataa gtatcggcat tacgttggta ggtttgtgca
     3301 tttgccggat cgtattttat caacgcatca cgaatattat cgacgtaaat cagagcatta
     3361 tctggcgaca tccatgcatg gggattaggt ttgccctcat agggtccttc ggtgatccct
     3421 actggcgtca cacccgaaga gacaattact tctggaaccc cattgagatg ctggtaaaag
     3481 cgttggaacc acaattccag attcatacca ttggcgagaa tcagttgtgc cccctgcgca
     3541 cgtttaatat cgccaggggt aggctgatac tcatgaattt ctgcaccagg cttggttatg
     3601 gatgagactt ctgcagcatc tccagccacg tttttggcca tatccgcgat gatggtaaat
     3661 gttgtaatga ccttgaattt ttcagttgca cttgcctgaa atgcgatcga gcaggtgagg
     3721 gcgaggcacc ccaagagcat ggttactttt tttattgagt gcataattta gtacctatag
     3781 tgagcaaaat tagaacaaac atagcaacgg ctatgtttta tagcataacc tatacaaaat
     3841 gaaaataatt atcattaaca gctttgaggc ctaaaacagg gattaggttg tttcctgctg
     3901 gcgacatgat attgaggaaa aaagtagagc agctattagg ctttgaaatg actttatgag
     3961 gaaaattgtt gatggcaaaa atggggcaaa acgctgtata aggggcaaaa aagagtgggt
     4021 tatcgtagct tattgttgtc gctgataatg tttaacatat t
//
LOCUS       DAETPZ010000202         4015 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.208, whole genome shotgun sequence.
ACCESSION   DAETPZ010000202 DAETPZ010000000
VERSION     DAETPZ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.208"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..73)
                     /locus_tag="K0W59_004170"
     CDS             complement(<1..73)
                     /locus_tag="K0W59_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HBI3024927.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRF"
     gene            complement(252..1610)
                     /gene="plaP"
                     /locus_tag="K0W59_004171"
     CDS             complement(252..1610)
                     /gene="plaP"
                     /locus_tag="K0W59_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HBI3024928.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGSIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1600..1680)
                     /gene="yoeI"
                     /locus_tag="K0W59_004172"
     CDS             complement(1600..1680)
                     /gene="yoeI"
                     /locus_tag="K0W59_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HBI3024929.1"
                     /translation="MRNPKKWGKFFAYATVITVKENNHVA"
     gene            complement(1878..2807)
                     /locus_tag="K0W59_004173"
     CDS             complement(1878..2807)
                     /locus_tag="K0W59_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3024930.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2853..3677)
                     /locus_tag="K0W59_004174"
     CDS             complement(2853..3677)
                     /locus_tag="K0W59_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI3024931.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3760..4014)
                     /gene="yoeB"
                     /locus_tag="K0W59_004175"
     CDS             complement(3760..4014)
                     /gene="yoeB"
                     /locus_tag="K0W59_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HBI3024932.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1096 a         1039 c          935 g          945 t
ORIGIN      
        1 aaaaacgccc acgctgcatg acaaatccca gcagagcacc acaaattagc ccgcttaata
       61 tcattgaaaa cataaattaa ttaaccagat gaatgttaat ggggaaattt ttcatgactg
      121 gtggaatgca gaccaataac caaattctct aaattagaac aaatggttat taatgaggat
      181 ttcgataaaa aagaataaaa aaaccggagc caaatgttca ttcgactccg gtttattatt
      241 agaaaagatg attatgctac gtcttcgtac tgcggaaccg gattacggaa gctcttggtg
      301 acgcaagcca ggtaaatcag accgatagca gcccagatca gacccagaac cattgagctt
      361 tcttccaggt taacccacag cgcaccaacg gtcagcgcac cacacatcgg caggaacaga
      421 tactggaagt gatctttcag cgtcttgtta cgcttctcac ggatccagaa ctgcgagatg
      481 accgacaggt taacaaaggt gaacgccacc agcgcaccaa agttaatcag cgccgttgcc
      541 attaccaggt cgaagttgat tgccagcagg gcaatcgagc caaccaggat gatgttcatc
      601 gccggagtac gccatttcgg gtgtacataa ccgaagaagc ttttcgggaa cacgccgtca
      661 cgacccatta cgtacatcag acgcgctacg cctgcgtgcg ccgccatacc ggacgccagt
      721 acggtaatgg tggagaagat cagcgcgcca acctggaacg ctttacccgc aacgtacagc
      781 atgatttcag gctgtgacgc atccggatct ttaaagcgag agatatccgg gaagtacagc
      841 tgcaggaagt aagttgcaaa gatgaagatc atgccgccaa tcagcgcggt caggaaaatc
      901 gcacgcggga tcacgcgctc tgcatctttg gtttcttccg acagattgct gatgccgtca
      961 aagccggtaa aggagaagca caggattgtc gccccggtaa tcatcgggat aacatgcgca
     1021 tcgccagacc agaacggacg cgtgctcgcc agcgtaccag cgccttcacc ttcaaatacg
     1081 ccataaacaa ccatgcccag aatcaccgcg atcagcacta cctgcaacac gacgattaca
     1141 gtgttgaagt tcgctacgga tttcagacta cgcaggttaa aggcggtcat aaaggccacc
     1201 agcgccacca caaacatcca cgatggaatg gaaggcacca gagcttcaaa atagattttc
     1261 gccagcagaa tgttgatcat cggcgcgaac agatagtcga gcagagaaga ccaacccacc
     1321 ataaagccga cagtcgggct aatggatttc tgggcgtaag tgtatgcaga gccagcagaa
     1381 ggatagcggc gaaccagctt cccgtagctc agagccgtaa acaggatcgc aatcaatgcg
     1441 aacgcatagg ctgtcggcac atgaccatcc gtgaggcctg aaacgatacc aaatgtatca
     1501 aacagcgtca tcggctgcat ataggctaga cccatcatta caaccggaac taacgtaagc
     1561 gttttacgca attccacgcg agaggtgttt ggagtaacgt tatgcgacat ggttattctc
     1621 ctttacggtg ataaccgtcg cgtaagcaaa aaatttgccc catttttttg gattcctcag
     1681 cgacaacaac tgtcgatttt tagtaaatat ctatccggta cgaagcccgg cctcttggta
     1741 tgaatgattg gtttgaagca aaaaataacc gacgctaatt aaacgtcggt ttttagtcat
     1801 tttttgacag cggcgcattg tgcctaaatg ggggggaaat gacaagagaa tgagaggctt
     1861 gtcataataa tttttcttta aatggctgat tttccgtcat cagattttgc ataaacaccg
     1921 gcgatcgcac tatttgctaa aatttcatct cgccaccagc cgctggcaag ccctgcggtc
     1981 tgttcattcc agccaatcca gacaggttca aagagagttt gggcgaccac tttcttctcg
     2041 attaacgcgc cactatcgag aaaacgttgc gccagatacc gtggtaaata gccgcagcca
     2101 aggccgctaa tttgcagctc cagtttggtt ttaaaatcga aaacggtaat ggcttcttgt
     2161 tcgtcgagaa gttgtgaggc cgtagaagcg cctgcctgcg cagtgtcccc caccacaatc
     2221 gcccgatatc gtttaatgat acgacggttt aacggttctt cttcctgggc taacggatga
     2281 tgaggcgcga ccgcaaagac ttgttcaaga tcgcccaacc gcgagaaacc aaactcactg
     2341 gaagatggtg gctcatgcat cgcgccaacg ataatatccg ctcgcccctg agtcagggca
     2401 tcccaggaac caccgagtac gccattgata aacttcaggc gcgtcacgct atgatgttga
     2461 taaaaagctt caatgagagg cgcaagcaga gaaaaaggaa acgtgtcgtc cacgccaatc
     2521 accagctcgt tttcccagcc ttcatgcagt ttgatcgcct gcttttccag ttctcgcacg
     2581 gtatgcagaa cttcccgccc tttctctaat aacatttttc cggtgcgggt gaatttagcg
     2641 cggtgtccgc tacgatcaag caattgaata tttaggtcgc tttccagctt gtgaacggtg
     2701 taactcagag cggacggcgt cttgtagagt ttggctgacg ccgccgcaaa actgccctct
     2761 ttttctatgg catcgaggat catcaacaca tccagcagtg gtttcataca cgcccccttg
     2821 cagtgcatgc ggcgtcctgc tggcgtgatc gtttactcca gcggcattac cagcggatcg
     2881 ggatactggt attcaaatcc cagttcatta caaatccgac tgccatcaat aatcttacct
     2941 ttgccgctgt ccagactatt tctgaactgc ggaggttgca gccccagtaa acgggccatc
     3001 tgcggataga aaacattacg cgcagggtga gctggcgcac atatattata gatgtgtccg
     3061 cctttaggtg cctgtaacaa cagagtgata gcgccaataa catcttctaa atggactaaa
     3121 ttaacaccat gttcaccatc aggggcggtt tttccggcaa agaagcgtcc cggatgacgt
     3181 cccggtccga ccaggcccgc aagacgcaga atatcgaccg aagtaccggg taaattgtgc
     3241 agccagtctt cgagttcttc taatactcgt ccactgttgg ttaccggatt acgcggggta
     3301 gtttctttaa ccgtgccttg cgcgtcgcca tagacagatg ttgagctggt aaaaataatt
     3361 cggggaatac gatgggccag cgcgctatcc actaactctt gtaccgcttg taaatagaac
     3421 tcatcgccgg ggccgctacg acgtgccgga agcgtaatga ccagcgcatc ggcatccata
     3481 agggcatcca gatcgtcaga atcgcaaact aactcaggct ccatgcgaag cagatagcta
     3541 tcaatgccac tcattcgggc cgcttcgaca ccatcttgtg tggttttact cccggtgact
     3601 tgccagcctc gcgctgaaag tgacatcgcc agcggcatac ctaaccaccc taacccgaca
     3661 attgcgacct ttttcatccg ttatctcctg atacacctta gatctataag gctacgctag
     3721 cgtatcaaaa ctgacaattc attctatgaa tgaatctgtt caataatgat aacgacatgc
     3781 tgcaatgagc agtgaatcat cagtaaccgc gtataccaga cggtgctcct ctgtaatgcg
     3841 tcgggaccag aaacctgaca aattatgttt caggggttct ggtttcccct taccttcaaa
     3901 tggtgttctg cgggtatctt tgataagttc attgatcttt ttaacaattc gcttatctgt
     3961 ttcctgccag tacagataat catcccatga ttcctcagac cagattagtt tcact
//
LOCUS       DAETPZ010000203         3977 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.209, whole genome shotgun sequence.
ACCESSION   DAETPZ010000203 DAETPZ010000000
VERSION     DAETPZ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.209"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..92)
                     /gene="yjbD"
                     /locus_tag="K0W59_004176"
     CDS             complement(<1..92)
                     /gene="yjbD"
                     /locus_tag="K0W59_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HBI3024933.1"
                     /translation="MRAPRITQKEMTEREQRELKTLLDRARIAHG"
     gene            complement(331..1680)
                     /gene="lysC"
                     /locus_tag="K0W59_004177"
     CDS             complement(331..1680)
                     /gene="lysC"
                     /locus_tag="K0W59_004177"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HBI3024934.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            2205..3854
                     /gene="pgi"
                     /locus_tag="K0W59_004178"
     CDS             2205..3854
                     /gene="pgi"
                     /locus_tag="K0W59_004178"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBI3024935.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT         1008 a         1050 c         1004 g          915 t
ORIGIN      
        1 ccatgcgcaa tccgcgcccg atccagcagt gttttaagtt cacgctgctc gcgttcagtc
       61 atctcttttt gggtaatacg tggtgccctc agtgagccag agagttcagt gtacgggaat
      121 tcgtttgcga gcagaactga taatattcat tttgccttct cttaactatt tatctctttc
      181 gataattaat tagagatgca gataaaaaaa ttaaaggcaa ttattctccg ataaagacgt
      241 tggttaacag agtacaggct cgtttattga tgagcatagt gacaagaaaa tcaatacggc
      301 ccgaaatata gcttccaggc catacagtat ttactcaaac aaattactat gcagtttttg
      361 caccacctgc tcggcatctt cgccgggcac caggaagcac aggttatggc tggatgcgcc
      421 gtaacaaatc atgcgaatgt tgaacggttc cagtacgccg aatacctctt tgccaacgcc
      481 gcaggctttt gacaggtcat tgccaatcaa cgcgaccagc gccagacctt cttccacctc
      541 cacccggcac agtgcggaaa gctccatcag cagagattgc gtcagcaacg tatcgccagt
      601 ggaggttgaa ccggtggtat caagggttaa tgccacgctc acttctgacg tggtgattaa
      661 gtctaccgaa atattatgcc gcgcgaggat gccgaaaact tccgcgagga agccgcgaga
      721 atgcagcata ttcaggctgt gcaaagtgag cagagtctga ttgcgacgaa gcgccagcgc
      781 gcggaacagc ggcggatttt cagttttatt gcacaccagc gtaccacctg cgcgtgggtc
      841 tttgctggag ccgacaaaga ccgggatatc gctgcgtact gcgggtagca acgttgccgg
      901 atgcagtact tttgcaccaa aagttgccat ctctgccgct tctgcaaacg cgatttcatc
      961 aatgcgtttt gctgcggaaa ctacgcgtgg atcggtggtg tagatgcccg ggacgtcggt
     1021 ccagatatca acacgagatg cgtgtaaagc ctccgccagc aaggctgccg tataatcgct
     1081 gcctccacgg ccaagcgtcg ttgtacgacc tttattttcg ctaccgataa atccctgggt
     1141 gatcactaag ccttcattga ggcgtgggag cagctgcagc gcggccagtt ccgccagcgc
     1201 ggctacatct ggctctgcac gaccaaatcg gtcgttggta cgcatcactt tacgtacatc
     1261 aaaccactgt gcctgaacat cgcgttcgcg caggatttcg acaaacagca gggtcgacat
     1321 cagctcgccg tggctgacca gctcatctgt cagcgccgga gacgttgcca gcgccgccgc
     1381 ttctgccaga acagtaatgt tctccagcag acgttcaatc tcttcacgga taacgttcgg
     1441 gtaacgcaga cgttccagaa tggcaaactg gatgttgcga atagcgtcga gtttttcgaa
     1501 tcgctcgcca ggttccagcc cttccgctaa agcgaccagc agattagtga taccagcaga
     1561 agccgagagg actactaaac gcacgttggc atcagaaagc acaatatcag cgctgcggtt
     1621 catggcgtca aaatcagcta cgctggtacc gccaaatttg gagacaacaa tttcagacat
     1681 aactacctcg tgtcagggga tccattttca gccttggcac aagggaagag cggaagacgg
     1741 gtgggcgcag agcgatactt cgctactatt ttcacccaga agtgctccac cacttgcgaa
     1801 acgcccgact gcgaacgctt ctggtgacaa cccaggggat tcagcccctg tagccgatga
     1861 tgaacgtggc cagccgttca atcacctcgg cgatgcaccc cctcaggtgt tatcacagga
     1921 ctggctcctc caacaccgtt acttgggcaa cgcgcctctt ctggcctgcg ctagcgcagg
     1981 tagtacattt ataaataaag ggtgagcggg gcggttgtca acgacggggt catgcggatt
     2041 tttcatccac tcctggcggt cagtagttca gctaataaat gcttcactgc gctaagggtt
     2101 tacactcaac attacactaa cggcactaaa accatcacat ttttctgtga ctggcgctac
     2161 aatcttccaa agtcacaatt ctcaaaatca gaagagtatt gctaatgaaa aacatcaatc
     2221 caacgcagac cgctgcctgg caggcactac agaaacactt cgatgaaatg aaagacgtta
     2281 cgatcgccga tctttttgct aaagacggcg atcgtttttc taagttctcc gcaaccttcg
     2341 acgatcagat gctggtggat tactccaaaa accgcatcac tgaagagacg ctggcgaaat
     2401 tacaggatct ggcgaaagag tgcgatctgg cgggcgcgat taagtcgatg ttctctggcg
     2461 agaagatcaa ccgcactgaa aaccgcgccg tgctgcacgt agcgctgcgt aatcgtagca
     2521 ataccccgat tctggttgat ggcaaagatg ttatgccaga agtcaacgcg gtgctggaga
     2581 agatgaaaac cttctcggaa gcgattatct ccggtgagtg gaagggttat accggcaaag
     2641 caatcactga cgtagtgaac atcgggatcg gcggttctga cctcggcccg tacatggtga
     2701 ccgaagcact gcgtccgtac aaaaaccacc tgaacatgca ctttgtttct aacgtcgatg
     2761 ggactcacat cgcggaagtg ctgaaaaaag taaacccgga aaccacgctg ttcttggtag
     2821 catctaaaac cttcaccact caggaaacca tgaccaacgc ccatagcgcg cgtgactggt
     2881 tcctgaaagc ggcaggtgat gaaaaacacg ttgcgaaaca ttttgcggcg ctctcaacca
     2941 atgccaaagc cgttggcgag tttggtattg atactgccaa catgttcgag ttctgggact
     3001 gggtcggcgg ccgttactct ttgtggtcag cgattggcct gtcgattgtt ctctccatcg
     3061 gctttgataa cttcgttgaa ctgctttctg gcgcacacgc aatggacaag catttctcca
     3121 ccacgcctgc cgagaaaaac ctgcctgtcc tgctggcgct gattggcatc tggtacaaca
     3181 atttctttgg tgcggaaact gaagcgattc tgccgtatga ccagtatatg caccgtttcg
     3241 cggcgtactt ccagcagggc aatatggagt ccaacggtaa gtatgttgac cgtaacggta
     3301 acgttgtgga ttaccagact ggcccgatta tctggggtga accaggcact aacggtcagc
     3361 acgcgttcta ccagctgatc caccagggaa ccaaaatggt accgtgcgat ttcatcgccc
     3421 cggctatcac ccataacccg ctctctgatc accaccagaa actgctgtct aacttcttcg
     3481 cccagaccga agcgctggcg tttggtaaat cccgcgaagt ggttgagcag gaatatcgcg
     3541 atcagggtaa agatccggca acgcttgact acgtggtgcc gttcaaagtg ttcgaaggta
     3601 accgcccgac caactccatc ctgctgcgcg aaatcactcc gttcagcctg ggtgcgttga
     3661 ttgcgctgta tgagcacaaa atctttactc agggcgtgat cctgaacatc ttcaccttcg
     3721 accagtgggg cgtggagctg ggtaaacaac tggcaaaccg tattctgcca gagctgaaag
     3781 atgataaaga aatcagcagc cacgatagct cgaccaatgg tctgattaac cgctataaag
     3841 cgtggcgcgg ttaatcatca tgcgtgcctg atgccgatat gtaggccgga taaggcgttt
     3901 acgccgcatc cggcaaccgg agcctgatgc caatatcgac attaatgcat agtatgagga
     3961 gttaaataac cttaaaa
//
LOCUS       DAETPZ010000204         3977 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.210, whole genome shotgun sequence.
ACCESSION   DAETPZ010000204 DAETPZ010000000
VERSION     DAETPZ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.210"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..1447)
                     /locus_tag="K0W59_004179"
     CDS             complement(<1..1447)
                     /locus_tag="K0W59_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBI3024936.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDRTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIPLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQ"
     gene            1831..2862
                     /locus_tag="K0W59_004180"
                     /pseudo
     CDS             1831..2862
                     /locus_tag="K0W59_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(2949..3425)
                     /locus_tag="K0W59_004181"
     CDS             complement(2949..3425)
                     /locus_tag="K0W59_004181"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024937.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(3418..3861)
                     /locus_tag="K0W59_004182"
                     /pseudo
     CDS             complement(3418..3861)
                     /locus_tag="K0W59_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            3916..>3977
                     /locus_tag="K0W59_004183"
     CDS             3916..>3977
                     /locus_tag="K0W59_004183"
                     /inference="COORDINATES: protein motif:HMM:NF015750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024938.1"
                     /translation="MASISIRCPSCSATEGVVRNG"
BASE COUNT         1172 a          757 c          714 g         1334 t
ORIGIN      
        1 tctgtttagt ttttttgagc gtctgtccac atttgaagat atcttcaggt tttggtctga
       61 aagcagtagt gttattaaaa taattttcat atacaggtgt gggtgctata gcattgcttc
      121 taattagatg gagaagatga tcatccatgt gttgtttatg ttttttatta aatatatttg
      181 ctaattcctc aggatcaatt gggtctttca tctgattaaa tggaacctgc aaagcaccga
      241 gacaataaca ttgattttct tcatttctta ttattccgtg atgactggtt aaaactttag
      301 tttctgagtt ataattacaa acatcgagcg ttttaattaa gttatacgta tcaccggcag
      361 agagtttata attttctagt cgtgcagtat agttaccatt aaaattgatt tcatgatttc
      421 cagccagaac gatgacattt ttattaatac ggctatcttt attaccctcc atatttcgca
      481 tagaatttag caaagtaagg atatatttat caccaaaaat agtactaaat ctatcgcctg
      541 tatgatcccc aagggggata caaggggtta tcgcatcgtc ctttaaaaaa acatgttgat
      601 gcaactcatt caatatcatg tctctcgtat ttaataaatt gtagagcaat ttattatttt
      661 tatgtatgta tgcagatgaa gccatgtctt cgtgttttaa taattcacat aataaactcc
      721 aacccttttc cctgatgcct atatgccctg aaacgagcaa tgaataaagt acagcgccta
      781 ctcttccatc tgtatcgcca aaataagccg tgttggcatt ttgtgggtta atcacaccac
      841 attgtttata aaagtctatt ttagaaaaca cagatttaac atagctatca aacttatcct
      901 taggaaacgt ctctttagag acatcccaac gccagtcagc tttaaaatta ccgctaagaa
      961 gagtaaagaa attttcaggt atgatagaaa gcccattaac tttataaatt atgtctgatt
     1021 tatctccgga gaaattaata aaataataac catccttaca ctcaatctta actgtaatgg
     1081 gagacgataa ttttatcaca tggttattgt ctggtataat tgtaaagata aacgtgttag
     1141 tagtattatc ggcaaaacta ttatattctt gaatatctat agatggctca ctattgtaaa
     1201 accatatttt ctccatctga agactatgtt catttaccct ccagtgacca tgttgcagga
     1261 aatagatgat atgcatgcca aaatcgttat ctatttgcga atcattatcc agtttatcaa
     1321 tttcaactat ctttgaacga tgtgttctat caaccttgct atggtgttga tgattattaa
     1381 ctttatctga atgcttatct ttatggtttg aagggatgta cttaaatata ttgtcaattg
     1441 ctttcataaa taacccgcga tatttttgac ctgttagtaa aaaataaatg atacatgtct
     1501 gaaagacata tttacgacga tataaaacaa taatacttat atgttcgctg attttaagca
     1561 ccgagactat ccggtctttt actaaataat ggaaaacaat ccatccctgg aagacaattg
     1621 taaaaaaaac aacagaataa gaattaatct taggataaat atttatttat catggccttt
     1681 tgaccgctaa aaaatggcac tcggcacagt aaaccccgct atagttatgc tatacatttg
     1741 tctttaatga tatctcgtcg ttatattaac aggctgaaat tatcattaat gcacatttca
     1801 atattagcag ggatacctga ggagtttaaa gtgaacatat atatcgggta gcttttcaaa
     1861 ttaatccctt tgattatagg cttaatttgc atcgcgttag gtggctttgt gctggaaagt
     1921 tcagggcaac gtcacgggtc atgtgctgat ttctctggcg gccatatgcc tggcattatt
     1981 cactaccgca tttattatca tttcgcagct cacgcgcggc gttaatacgt tttacaatac
     2041 attgttcccc attattggct atgcggggtc aattatcacc atgatatggg gttgggcact
     2101 gttagcaggt aatgatgtga tggcagacga gtttgttgcc ggccatgtta tttttggcgt
     2161 tggtatgatt gccgcatgtg tatcgacggt ggcagcgtcg tccggtcact ttctgctcat
     2221 tcccaaaaat gcagcgggga gcaagagcga cggaacaccg gtacaggctt attcttcatt
     2281 aatcggtaat tgcctcattg ccgttcccgt tttactcacc ctgctcggtt tcatttggtc
     2341 tattacgctg ttacatagtg ccgacataac tccgcattat gtcgccttgg gttaaccgca
     2401 atctgtgcct gtctaattgg cctagtggcc acaatcgtcc atcaaacacg taatacgttc
     2461 tcatccaaag agcactggct gtggtgttat tgggttattt ttctcggctc agtcacggtg
     2521 ctgcagggga tatacgtctt agtcagttcc gatgcaagcg cccgactggc tcccggcatt
     2581 attctcattt gcctcggaat gatctgttac agcatattct caaaagtctg gctactggca
     2641 ctggtctgga gacgtacctg ttcgttagcc aacagaatac cgatgatacc ggtatttacc
     2701 tgtctgtttt gcttgttttt gtcatcattc ctcgcggaaa tcgcacaaac tgagctggac
     2761 tactttattc catctcgggt tctggttgga ttaagcgcag tatgttttac attgttctct
     2821 attgtttcaa tattagaggc cggttctgcg aaaaaaaatt aaaatttaca taatcccagc
     2881 gttattcata tcttaacgct ggttttttac gaacttagtg aaataatgta gaatataaaa
     2941 ttataatttt atattggagc acatgtgtta atgatcaacc ccgggatact aaaaatatag
     3001 acattattaa acttgcttag cctcgcattg cctttacctg ttaaagatgc atcgagaaaa
     3061 ttgagtctga agttctcatc ctttatctga ttttccagtc ccgaaacttt ggccttagca
     3121 ttacctgaga tccgtccgtt actatctatt ttgtaggtaa aaaaatacga atgggaaaat
     3181 ttcctcggtg tatcttcatc agttaagcca acaatcgtaa aactaccgct accatctttt
     3241 tttaaaatga ctgaaaggtt agcagagtag tataaagaat gatctgtttt agcatttctt
     3301 gttaacgtcg acgtacattc aagagacgtt gtgctaccat cacgagcctt ccagagagca
     3361 aaaaaacaca cctgtagcaa tgtgaaaaca agtattaata taaaatacat tttcttatta
     3421 gtcatataat agcacctctg aacaaattgc ataagtttcg tcattagagg attttataca
     3481 tcttgataaa taccgctgcc cttccccaag caaataatgc atactattta catcatatat
     3541 aaaatttgca cctggcacac agggtaaatg ctttttcgaa atttcagcgg cctctatttg
     3601 agatttagcg aggccaatct cgttaagatt ctctgaaaga tataaatcac aggtgtcaat
     3661 cttacctgat ttaaccaatg gataatccgt tatactgtag gtatctttaa aagccacggc
     3721 aaacatttca ataataacaa ttactaccac aagcgaaatc aaaatgcgaa aaagaggaat
     3781 actccttttg cgctctactt tttcaacaac atcttcttgt aaatgagaat catcataaga
     3841 ttgtttactt attgttatgt ggtaatgctg ccaacttact gatttagtgt atgatggtga
     3901 ttttaaggtg cttgcgtggc ttccatttcc atcagatgtc cttcctgctc cgctactgaa
     3961 ggcgtggtgc gtaacgg
//
LOCUS       DAETPZ010000205         3765 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.211, whole genome shotgun sequence.
ACCESSION   DAETPZ010000205 DAETPZ010000000
VERSION     DAETPZ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.211"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(344..610)
                     /gene="ospE2"
                     /locus_tag="K0W59_004184"
     CDS             complement(344..610)
                     /gene="ospE2"
                     /locus_tag="K0W59_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HBI3024939.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(792..1193)
                     /locus_tag="K0W59_004185"
                     /pseudo
     CDS             complement(792..1193)
                     /locus_tag="K0W59_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1622..2410
                     /gene="virF"
                     /locus_tag="K0W59_004186"
     CDS             1622..2410
                     /gene="virF"
                     /locus_tag="K0W59_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HBI3024940.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(2403..2774)
                     /locus_tag="K0W59_004187"
                     /pseudo
     CDS             complement(2403..2774)
                     /locus_tag="K0W59_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            2773..3527
                     /locus_tag="K0W59_004188"
                     /pseudo
     CDS             2773..3527
                     /locus_tag="K0W59_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1085 a          754 c          768 g         1158 t
ORIGIN      
        1 cccctaagcc gtctatggga aggagtctgc tgttattcca gagaatggta attcagtcat
       61 atatttctcc atctatgact tgtcaatacc atcctgatat ttgtgggtca gcaatatatt
      121 ctactaaaag gtatttaagg tcattacgtc cctgcactcg aataatggtg aagaaagcat
      181 gataaatata tcacgataac gttcagtatg tatcgaggat agatggagtt actcctctct
      241 gaaatagaat tactgctcta ctttttctgt ttccagaaat tattagtaac aaaccctgat
      301 atattatttt gaaaatggag cttaaaccag tctgaaatta acttcagaaa tcagaatacc
      361 gttggctttg tttttcatca attccggctt catttatctt tgtcaaccat tgcatttctg
      421 tagcagtgaa tttcaacgtt ttcgtccggt tggcagcagg tatttgtaat tcgtatatag
      481 ctgctttttt attgaaaaca gtatatccat ctgtagtaac agtagaagat aataatacgg
      541 gatttgatac ttcaaggatg atattttctt gtttttgtgg aaggcaaggg aatatagttt
      601 gtgtaagcat attgcttcct tatgttatat tttagtaaag aatttatttt ttacattata
      661 gtcggctaaa tgaagtttac tgtaaaaaaa cgatactttt ttcgtaagcg atgagatgtg
      721 gagccagaaa aatggctgcg ttacgtcatt gaactgtcct tgacccacga tattcagaca
      781 gtcgttgtat cttaagttga cattacccgc tgacgtcgat attcccttgg tgagagataa
      841 cccaacgcac tatgcggatg gttttcattg taatgctcga tcgccactgc aaggttatgc
      901 aatgccgttc ttggtttcgg catgaacgcg atgcagtctt ccttcatcgt tttcacgaac
      961 cgttctgcta ttccattact ctgagggcta ctgactgccg tcgtgcaggg ctccagattc
     1021 aactctctgg cgaactgccg tgtttcatga gccgtataag ccgaaccgtt atcggccact
     1081 ggattggccg ttccggtcct ttatcgccat atcgctcctg acatgacatc ctgcaccgtt
     1141 gctttgtcat aatctcccgt gcctgctgcc cagtctatgg cttcacgatc gcaatatgga
     1201 acgtaacgct gtcatcggga gaatcgtcaa tgcggcattc ggttaacgct taccaatatc
     1261 aacaccaacc ggatttgtgg agcctccagt ctgaaggggc tttatgcgtt ccgtatagga
     1321 tattatgatg ctggagtttt gcgaagctcc actcctttcc gaacatttac acagatattg
     1381 cctaagaaaa gtgaaaccat aatatggtta tagtcccttt cagtgcaaat acttagcttg
     1441 ttgcacagag aaatagaagc tgcataagct ctttcttcaa aaaatgtaaa taaagttaaa
     1501 tataggaaaa attacttaat ctatcttaat aacggaaaga ttttgtatac aatcactgtt
     1561 acagaaattt cttagttact ctgtaaacac taaatatagt ttggtatatt ctgttgaatt
     1621 tatgatggat atgggacata aaaacaaaat agatataaag gttcgcttgc ataactatat
     1681 tattttatat gcaaaaaggt gttcaatgac ggttagctca ggcaatgaaa ctttgactat
     1741 cgatgaaggg caaattgctt ttatagagcg aaatatacaa ataaacgtct ccataaaaaa
     1801 atctgatagc attaatccat ttgagattat aagccttgac agaaatttat tattaagcat
     1861 tattagaata atggaaccaa tttattcatt tcaacactcc tattctgagg agaaaagggg
     1921 gttaaacaaa aaaatattcc tcctctctga ggaggaggtt tctatcgatt tgttcaaatc
     1981 tataaaagag atgcctttcg gcaaaagaaa gatctatagt ttagcttgcc ttttatcagc
     2041 tgtttctgat gaggaagctt tatatacttc gatatcgata gcttcttctc ttagtttttc
     2101 tgatcagata aggaagattg ttgaaaaaaa catcgagaag agatggcgtc tttctgatat
     2161 ttcaaataac ttgaatttat cagaaatagc tgttagaaaa cgattggaga gtgaaaaatt
     2221 aacatttcaa caaatccttc ttgatattcg catgcatcat gcagcaaagc ttttattgaa
     2281 tagtcaaagc tatattaatg atgtatcaag acttatcgga atatcaagcc catcttattt
     2341 tataaggaaa tttaatgaat attatggtat aactccaaag aaattttact tatatcataa
     2401 aaaattttaa atgcttcata gcccatcgct attgccagat gggttttccc cacacctgat
     2461 ggccccagta atacgatatt ttcattacgt tctatgacgc tgagtgagcg taacgactgg
     2521 agttgcttct gcggtgctcc ggtggcgaat gtgaagtcat actcttcgaa cgttttcacc
     2581 gccgggaagg ctgccattcg ggtatacatc gcctgtttac gttgatgacg tgccagtttt
     2641 tcttcatgaa gcagatgctc caggaagtcc atataactcc attcctggtc tactgcctgt
     2701 tgtgacagcg caggcgctgc gcttatgtac tgcacccatt ttgttggacg atgaaatgga
     2761 atagcccctg atatgtcaaa gccaaaatac ccttttgaaa agcgccttga agtcgtgaat
     2821 cactacttca ctactgatga tggttacagg atcatctcgg cacgttttgg tgtcccccga
     2881 acccaggtca ggacatgggt tgccctctat gaaaaacatg gagaaaaagg cttaattccc
     2941 aaacctaaag gtgttagtgc tgatccagag ttgcgtatta aggtcgtgaa agctgtgatt
     3001 gagcagcaca tgtcccttaa tcaggctgct gctcacttta tgcttgctgg tagtggttct
     3061 gtagccaggt ggctgaaggt ttatgaagag cgcggagaag ctggtttacg cgcgctcaag
     3121 attggcacca aaagaaacat tgcaatatca gttgatccag aaaaagcggc atcagcattg
     3181 gagctgtcaa aagaccgacg cattgaggat cttgaaaggc aagttcgatt tcttgaaacg
     3241 cggcttgtgt atctaaaaaa gctgaaagcc ttagctcatc ccacgaaaaa gtgaaagtac
     3301 tcaacgagct aaggcagttt tatcctcttg atgagcttct cagggctgcg gagataccgc
     3361 gcagtacgtt ttattaccat ctaaaggctc tcagcaagcc tgacaagtat gcggacgtta
     3421 aaaagcgtat tggtgagatt tatcatgaga atagaggccg atacggatac cgtagggtaa
     3481 cgctgtctct tcatcgagaa gggaaacaga ttaaccataa agctgttcac gtataagaaa
     3541 ttgaaaaacc agacgatacc gctgtccacg ccttatgcca cgtgagcttt tctgcttttc
     3601 cctccactcc ggtgttttct ctctctgcat ggtcgattct tataccgcaa caggctgttt
     3661 tttatacttg caccgattcc cgggcacact tagcctgtgt caggccggca tatacctcag
     3721 cagactgata tcccgcgcgt ttttcttcag cccctccaca ttcag
//
LOCUS       DAETPZ010000206         3542 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.212, whole genome shotgun sequence.
ACCESSION   DAETPZ010000206 DAETPZ010000000
VERSION     DAETPZ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.212"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..221
                     /locus_tag="K0W59_004189"
     CDS             <1..221
                     /locus_tag="K0W59_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024941.1"
                     /translation="ANFIALQINGSKTGFAASITGFTQTISAGTISFLSVTVSDNGVG
                     PGTTLGLGSLITSLLSIAVIAIAGNNKK"
     gene            938..1126
                     /locus_tag="K0W59_004190"
     CDS             938..1126
                     /locus_tag="K0W59_004190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024942.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            1223..1402
                     /locus_tag="K0W59_004191"
     CDS             1223..1402
                     /locus_tag="K0W59_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024943.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            1454..1648
                     /locus_tag="K0W59_004192"
     CDS             1454..1648
                     /locus_tag="K0W59_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024944.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(2447..2783)
                     /locus_tag="K0W59_004193"
                     /pseudo
     CDS             complement(2447..2783)
                     /locus_tag="K0W59_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1131 a          760 c          693 g          958 t
ORIGIN      
        1 tagccaattt tatcgcatta caaataaatg gttcaaaaac aggatttgcc gcatctatta
       61 caggttttac tcaaacaata agcgctggta caatcagttt cctgtcagta accgtaagcg
      121 acaacggtgt tggtcccggg actactttag gtcttggttc actaataact tcattactgt
      181 caatagccgt aatcgccatt gcaggaaata ataaaaagta agtgtactct gactgcgtat
      241 gtatctactt tgtgtaatgc aggtgtctat tgaaaataat tcttttcgtg ggcatctgtt
      301 gtataaaata aaaaattatc gtaagtactc tatctgcgat gtcttgtgaa aattatcctg
      361 tctggcaaca atcgcgtcca tctgtattaa tggacgcgaa cgatgaactc tcaaacaaca
      421 ctagatatca ccggctttcg cacttatttg ccagaatcat tacgtctcag attcgacgag
      481 caattatctg tccgggctat cgcacagcag ttaagactca gttattcccc cattcacaat
      541 ctctttcaac gaattaacgc ctcaggtatt gtctgaccat gacccgagtc cctttcattc
      601 gcccaaccga taccatactt tatgccaaca gaaagaatga atgaaccgcg cctaaaatca
      661 gcaaggacac atggcacaaa gtgcgacatg tctcacattt aaccccagtg ggggcaacaa
      721 gtataaaacc gttttttgat taagtggtcg aatatgaaaa ggggaaggtt tatgtgaaaa
      781 atacctgtgt tttctttgaa aacacacaac agtatcaggt tatttatgtt tatacagtgc
      841 gttccaaact gacctgaata attacgcaag ttgcaacaat aaaacatttt tgccgtagtg
      901 tgaggtactc cggtaaattg ctttccaggt gcagacaatg actaacgaag aactacaggc
      961 aatcgcatta tccatgcttg agcgccagcg cgaaaaagta caccagcaac gagagtcttt
     1021 gcttaaccca tctccattca ccaggccaaa acgattggag cttgaagcat tcttggacaa
     1081 atacccacgg cggcttaaat caggcaaaac cagaccacca ggctgatagc tcacagtttg
     1141 ttctccgatt caataaaacg gataagaacc atcagcgcgg tagcgtaagg cactacagcg
     1201 cattgcttta cgagtaataa caatggattt atcaccagaa gacgcaaagt ttttacgaga
     1261 actgcaacac cgtatgctca gagaagccat tatcgaacag caaacgggca tgagcgccat
     1321 agacccattg cgatacaaac gggcctcatc tcctttagtt gacgacaatt ccaatgcgga
     1381 taaaacacgt ccaccacact gattacattt ttcattagca cagtggtgtg atgcagcata
     1441 cgaggaaaac cggatgaatg agaacaaaat aaaaaggctg gagcaactgt tacaggcacg
     1501 ccaacgggag tttgccacta aatggggacg tgccgcgctg ccacatgaac gcctaaccta
     1561 ttttccactt gaagacctca ttaaaaataa tggcaaaaaa atcaggtctg ataagaaaca
     1621 cttcttagaa tgcaatgaag acaaataaca acaatctgca tattgccacc aaccacagat
     1681 gtggcatcgt tatcagcgca cttgattttc tttatatcca ctgacgagct tgccggggca
     1741 cctttgtttg ccggtcattc tggtatcatg cagatattta cggcgtacaa gcccactagc
     1801 atgacattgt aataaggcct gagcctaacg ctttaatgtg tgtcatacac tcagtggcgt
     1861 aatcgttgac aatatggtgc atgtaatcgt aagcgtccag cgaaatccat ctggtcataa
     1921 tctgaagcat ccgacaaagt ggtgtccacc aaataagtga tctgcgccca atatcctgag
     1981 ccggaacttt gacaaaggcc aacgaaaaat gggagcggat gttacagagt tctcggtaca
     2041 ggggaaaagt ggtacctgtc gccagttctc cgatctgttt aaccgggaaa ttatctccta
     2101 cagcttgtca gaaaggtcgg tgatggagca tcgttaatac gatgctatac gatgcattca
     2161 cagtgcccgg ccagaggatg ccccgtcgct gcatatggat cagggttggc aatatcgaat
     2221 cgcaggctat aggcaaagtt aaagccccat ggagtagcac aaaatatgcc gtacaaagga
     2281 aacggtctga ataacgcagt gatgaagaac ttcttcagca cactgctaaa acgcatagtg
     2341 atcgagcgct gattctggcg aacaactgaa ctaatacatc agaatctgca ttatgttaaa
     2401 taaatatgaa aagatggttt aaataccccg ttacttttga cttactctat acggtatcgc
     2461 atcgtttaat attcgcaccg gccagatttt tatttctatt agttgtcaca atactgaatg
     2521 cgtacgacca cagtattctg gctcctgtgt ggttatgctt taattctgcg ttccgggcag
     2581 ataagcagtt gcttgcagga atccttcttg tgttaatgtc agttcccctt tttaccagtg
     2641 ctgatttcca cattccgtcc aacagagctt atagcctttc cctggattat agcattgtcc
     2701 ggctgaagtt ctttttgaat aataatagaa gcactgctgg cagatccagt ccgtttttca
     2761 taacccactg tactgataac cataatctaa tcagtagaaa ttgagtcgaa aataagcact
     2821 actccataca ggataattag aggtcagttt gattattcac aattcatcat cagcattttc
     2881 tatttctgac gaaatcaaca tgaaaataac catatatgat aattattata ataacggctt
     2941 taattggaat acatatatta caacgtatta tatataattg gtattctggg aactatattc
     3001 tcaaaataca gtagaaacga ggtatgtttc tggtggaaag gacagtggga ttaaaaagta
     3061 agaattgata aaaaaaacgc cagcaacaca tagtgccggc ggagggaata ccccatggag
     3121 aaaatgtgat gcctagagca tcatgaatat ataattaaaa atagttagcg ttgtcaatac
     3181 tttcacaaaa ataattttcg tagtatagaa agatattttt atgcatgacc tacctgaatt
     3241 tgctccgggt agaggttata aataaaaatt gaatcacgac aaacacaata ttcatagtat
     3301 ggcgatgcct acgccagcaa gaatagcgcc aataatattg ggaatataat agacaccaga
     3361 cgcacaggca tctcactcct taacaaacaa taatcaggat atttactttt acccagctaa
     3421 ctgtttacac ccaaagtgca cacaattaac cattcaataa caaatggcaa tatccatagc
     3481 catacgactt taccttgtaa tgttcggtat ttctttataa ttattctggg aaatctatca
     3541 tt
//
LOCUS       DAETPZ010000207         3522 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.213, whole genome shotgun sequence.
ACCESSION   DAETPZ010000207 DAETPZ010000000
VERSION     DAETPZ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.213"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(66..1508)
                     /gene="cusS"
                     /locus_tag="K0W59_004194"
     CDS             complement(66..1508)
                     /gene="cusS"
                     /locus_tag="K0W59_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HBI3024945.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            complement(1498..2181)
                     /gene="cusR"
                     /locus_tag="K0W59_004195"
     CDS             complement(1498..2181)
                     /gene="cusR"
                     /locus_tag="K0W59_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HBI3024946.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2338..>3522
                     /gene="cusC"
                     /locus_tag="K0W59_004196"
     CDS             2338..>3522
                     /gene="cusC"
                     /locus_tag="K0W59_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HBI3024947.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEKAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQ"
BASE COUNT          867 a          911 c          921 g          823 t
ORIGIN      
        1 ataggtatga ctattgcggc acgttatttt tacactggtt ataaaagttg ccgtttgctg
       61 aataattaag cgggtaatgt gataacaaac cttgtccccc gcgcatctga cgttaccgca
      121 accgtgcctt tatgcgcgac aacaatcgat ttcactatcg ccagcccaat accgctacct
      181 tcaccttttc gctggcggga ggggtcaacg cgatagaaac ggtcaaacaa tcgcggtaag
      241 tgctcgggcg caatgggcgt accggggttt tcgacggaaa cttgcacctg gtgatcgacc
      301 gtctgacagc gcactacaat tgtctctctg gttggcgtat aacgcagggc attagaaagc
      361 aggttgctta gcgcccgacg cagcatcaac ggatcgcccg caacctgacg tttgtcgcca
      421 acaaaccgca actccacgcc gcgatcttcc gctagcgcct cgaaaaaatc gaacactttg
      481 ccgacttcat ccgccaggtt gagcattttc ttttcgggga ttagctggtt gttatcggcc
      541 tgagcgagaa acagcatatc gctgaccatt ttcgccattc gcgtcagctc ttcgagatta
      601 gagtagagca catcttccag ctccttctgg ctgcgcgact ggctgagggc gatttccgtt
      661 tgcgtaatga gattagtaat tggtgtgcga atttcgtggg cgatatccgc tgagaaattg
      721 gactggcgag taaagacatc ctcaatacgc tcgatcatat ggttgaacga cagtaccagc
      781 tgttccagct caatcggcac ggtctgcggg tcaaggcgaa cgtcgagatc tttcgaggtg
      841 atattctgga tttgacggct gacgctgcgg atcggcgcgt gacctttatg taccgccaac
      901 agcacgataa agacgatcag gatgctgatt accgatgcgg tcataataag tttattcatc
      961 aaatcattta tgtaatgaag atgaaaatcg atcgacagcg cgatgtagag cgtataaatc
     1021 ggtttgccgt ccaccaacgg gccaaccggc aagttaatca tccgccagtt gctgtgttcc
     1081 atatgcccgt gaccgtggcc tggcatcata atcgtcgggc cggaaaggag atacacctcg
     1141 ccaccccgcg cgtctttatc gggtatggca tcacgtgcaa actcgcggat atcaggcgca
     1201 ccgggggagt gatacaccgt tttgccgtgg ctatctgcca gggaaatcaa cacgttggaa
     1261 taaccactga cgatatcttc cagcgtcatt aagcgtcggg cttgcgtttc gtcagggtga
     1321 tttagtaccc gttcaagggt ggcactaatc tcttttaaat cattaatgtc ctgctcggca
     1381 aaatgcactt ttactgagtg gatcatgatc catgcaaagg cgaaaaacgc cgcgatggtg
     1441 gccaggctga taaaaaaggt caggcgggtt gccagcgaaa acgggcgctg aaatggctta
     1501 ctgaccatcc ggcacctcaa gcatgtaacc cacgccgcgc acggtttgaa tcagcttcgg
     1561 ctcaaagtcg ttgtcgattt tgccgcgtag ccgcttcacc gccacatcaa tggcgttagt
     1621 gtcgctgtcg aaattcatgt cccatacctg cgaggcgata agcgagcggg gcagcacttc
     1681 gccctgatgg cggagaaaaa actccagcag agtaaattct ttactggtca gagtgatgcg
     1741 cgtgccgctg cgggtgactt tgcggctgac gagatcgacc atcagatcgg caacctgaaa
     1801 ctgactttcg ataatcaccg ccgccccgcg ccgcaggaga gtgcgcaccc gcgccagcag
     1861 ttcagcaaaa gcgaacggtt tcaccaggta gtcatctgcc cccaactcca gccccttgac
     1921 gcgatgttca atggtgccaa gcgcggtaag caacagaatc ggcatccctt tattggcgga
     1981 gcgcaacatg cgcacgatat cccagccgtt cacgtctggc agcataatat cgaggattat
     2041 caggtcataa tcaccggtca tcgccagatg gtagccattc agcccgttgt cggccaaatc
     2101 gaccacaaaa ccggcttcgg ttaacccttt ggtcaagtat tctccggttt tcttttcatc
     2161 ttcgacaatc aacagtttca tatttcctcc gcatgttgcc cgggcaattc tagagtagcg
     2221 ggatcagatg gcaatcgctt attggcaaaa tgacaatttt gtcattttcc tgtcaccgga
     2281 aaatcagagc ctggcgagta aagttggcga cataaaatca ccagaaatta tgagcctatg
     2341 tctccttgta aacttctgcc attttgtgtg gcccttgcgc taactggttg ttcactggca
     2401 ccggattatc agcgtccggt aatgcccgta ccgcagcagt tctcactcag ccagaacggc
     2461 ctggttaacg cagcggataa ctaccagaac gcgggctggc gcaccttttt tgttgataat
     2521 caggtgaaga cgctgattag cgaggcgctg gtgaataacc gggatttgcg catggcgacg
     2581 ctgaaagtgc aggaggcacg ggcgcaatat cgtctgaccg atgccgaccg ctacccacag
     2641 ctcaatggcg agggcagcgg cagctggagc ggcaatctta aaggcgattc agccacgacg
     2701 cgggagttct cgactggcct taacgccagc tttgatctcg attttttcgg tcgcttaaag
     2761 aacatgagcg aagccgagcg ccaaaattat ttagccactg aggaagccca gcgcgcggtg
     2821 catattttgc tggtttctaa tgtcgcgcaa agctatttca atcagcagct ggcgtatgcg
     2881 caattgcaaa tagccgaaga aacgctgcgt aattatcagc agtcatatgc gtttgtcgaa
     2941 aaacaactgt tgaccggtag cagcaatgtt ctggcgctgg aaaaggctcg cggggtgata
     3001 gaaagtaccc gcagtgacat cgctaaacgt cagggggaac tggcgcaggc gaataatgca
     3061 ttgcaactgt tattgggaag ctacggcaag ctaccgcaag cgcagacagt aaacagcggc
     3121 agcctgcaaa gcgttaaatt accggcgggc ttgtcgtcgc aaatcttatt gcagcgcccg
     3181 gatattatgg aagctgaaca cgcgttaatg gcggctaatg ctaatattgg tgctgcgcgt
     3241 gcggcatttt tcccgtctat cagcctgacc agcggaatat caaccgccag cagcgatctg
     3301 tcgtcattat ttaacgctag cagcgggatg tggaatttta ttcctaaaat tgagatcccc
     3361 atttttaatg ccggacgcaa ccaggccaat ctggatatcg ccgaaattcg ccagcagcag
     3421 tcggtggtga attatgaaca gaaaatccag aacgccttta aagaggtggc agatgcgctg
     3481 gcattacgtc aaagcctgga cgatcaaatc agcgcccagc ag
//
LOCUS       DAETPZ010000208         3519 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.214, whole genome shotgun sequence.
ACCESSION   DAETPZ010000208 DAETPZ010000000
VERSION     DAETPZ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.214"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..172
                     /locus_tag="K0W59_004197"
     CDS             <1..172
                     /locus_tag="K0W59_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3024948.1"
                     /translation="RLYGILNAMRHGVSNGNAEALNSKIRLLRIKAKGYHNRERFKLG
                     VMFHYGKLNMAF"
     gene            196..602
                     /locus_tag="K0W59_004198"
                     /pseudo
     CDS             196..602
                     /locus_tag="K0W59_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912930.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1186..1902
                     /locus_tag="K0W59_004199"
     CDS             1186..1902
                     /locus_tag="K0W59_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024949.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGVLFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVRKHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2454..2858
                     /locus_tag="K0W59_004200"
     CDS             2454..2858
                     /locus_tag="K0W59_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024950.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAIPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2921..3519
                     /locus_tag="K0W59_004201"
                     /pseudo
     CDS             2921..3519
                     /locus_tag="K0W59_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          989 a          730 c          825 g          975 t
ORIGIN      
        1 aaggttgtac ggtatcctga acgccatgcg ccatggcgta tcaaacggaa atgcggaagc
       61 actgaacagc aagatcaggc tgctgagaat aaaagccaag ggctaccaca accgggagcg
      121 ctttaagctg ggagtgatgt tccactatgg gaaactgaat atggcgttct aagccttccc
      181 accatgatcg gggaagaccc agatatgaac aggcacattc agagttactt taaagagctt
      241 actgaacgtc tgaggtgtta gagccatgac tgccgcagca ttgctggcgg aaatgcctga
      301 atcgagcagc ctctcgagac gtgaaatcag cgcacttgtc ggtgttgctc aggtaaaccg
      361 ggactcggga actctgcgcg gtcgacgaac cattttcggt gattgtgctg gggaagaaca
      421 gctctgtaca tggcggcgct tgaggccccc ggtttaatct ggtgataaag gcgttttaca
      481 tgcgcctact tgcagccgga aacgccaaaa aagtggcact ggttgcctgt atgcgtaagt
      541 tgctgaccat tatgaacgca atgctcagaa aaaacgaaga atggaatgaa tcgtatttat
      601 gaaaactgga ttgtgggcca taaatattcc aaaaagatag cccgctccgt ttattaccgg
      661 aacgggcgag agcatgacat tgtctgttat tataatgaga ctgctgattc tacttatttt
      721 taatttcaag aaaagcgttt tctacaaaga attatgagat ttaatgtttt gtttagacaa
      781 tattatcatt ctgttcaata atgaaagtct tcatcaacaa caagtaaaaa caacatccct
      841 ctcatcgaac cggacttgca gatttcccgc atccggctct cttgcaacac atcagacctt
      901 cacgttcggg cggttatggg gaaggtccag caagctgtat gagccctaac tgtttgaata
      961 ccagtagatt accagaaact tctatcgatt gaagacatta ccaactagcg ttactaaatg
     1021 ttgaccaagt tttgttcact tgtctggttt tgatagtagc attctgcaga gtattttgat
     1081 atgataaata tgcattaaca atgatattat tgttaatttc ctagcataaa aattaaaaga
     1141 tcaactttta tctcgaatat tttttacatt atcagttggt ttcttatgaa tgacaattca
     1201 ttgcttagaa attcttcatt attcatcgcc tatatgggat gtgtagggtg ggtcagtgct
     1261 tattcttatg gctggggaac ctctttttac tatggatttc catggtgggt tgttggtgca
     1321 ggtttggatg atgttgccag aagtttactc tatgctatca tagtaatggg tatacttttt
     1381 accggttggg ggattggtgt tttatttttt cttcttataa agaaaagaag taaaatacaa
     1441 gacttgagtt tttttagact attttttgct attaccctac tgtttttccc tgtaatattc
     1501 gaacttttaa ttctcaaaca atatttcata cttccattat cactttcatg cattattagt
     1561 tcattggtta tttcaataat aattcgtata tatggacgga tcttttctgt atcatgtttt
     1621 tcagatatcc cttttgttag aaaacaccgc attaagttaa ttatggctgg ttttttggtt
     1681 tatttttggc ttttttcctt tcttgttggc tggtataaac ctcaattaaa aaaagaatat
     1741 cagatgcttt gttataataa tagttggtat tatgttcttg ctcgttatga ttcacggctc
     1801 gttttgtcta gttcatttaa agatgacagt aacagattct taatatttaa tacagaacag
     1861 tcgggatttt atgaaatcaa tgatgtttat gtcagaaagt aataatcctc tcccacaaag
     1921 tgaattaaat gaatatataa tgcgttcgaa gtatatgtat tgagctgtca tactgagaaa
     1981 caaaccttag ggcagcacaa ttaaagccag aaacaactat gcaagaaagt tactaaataa
     2041 gtgctcggaa cagtaaaaca ctcagggggg actcagtccg gattatgtaa tcagataaat
     2101 tgccaaacat cacaaatcac cagccggact gagtgatgcc aagcattgca ccaatacccc
     2161 gtgatgaacg atgcctgatg cagaaagctc cctataaaac aaagaacaaa aactacgacc
     2221 ggcgattcac cgtcatactg atgctgcatc gtggtgacac tgtctgtcac tttcatgttg
     2281 actgaacaac tctgctgcga agaaggtgta ccgccggcgg gatttgattg tccggcgtct
     2341 agcttccact tccctgatgg aactgaaagt tgttatggcc ggtcagcttg ccctgtccgc
     2401 aggacatgag gtgctgtgac ggacagcacc agtaacggaa agtgacaatg aaaatgaata
     2461 ctgcgctgat agttgcacta atgtgtatgt ggtatgcggt acccgctgct gcaaaagaga
     2521 ctttactggc tataccacgt aattcaacag aacattgcta tgcggagatt aatgtgcacg
     2581 gaccatatgg ggtgtatttc cgagtggtcc cgcatccccc cgggggaaag agctgggtcg
     2641 aatgtaacag tgactactat tactctgata aaccaccggg agtacagata ctcggaaccc
     2701 gtgcagggtg tcgggtatac ggaatctgcg ggaccacctc gacactgcat gtggctggaa
     2761 gaggggttgt gtgtattaaa aatatatgca gtccacgtgg gatgataatt cacaggatac
     2821 ggaaacgtcc tgtagtggct gtaagcgatg agatgtgatc ttacccagca atagtggaca
     2881 cgcggctaag tgagtaaact ctcagtcaga ggtgactcac atgacaaaaa cagtatcaac
     2941 cagcaaaaaa acacgtaaac agcattcgcc tgaattttgc agtgaagccc tgaagcttgc
     3001 tgaacgcatc ggtgttgctg ccgcagcccg tgaactcagc ctgtatgaat ctcagctcta
     3061 tgcgtggcgc agcaaactgc agcaacagat gacctcgtct gagcgtgaaa gtgaactggc
     3121 cgctgaaaat gcccgcctga agcggcaact ggcggagcag gcagaggaac tggctatcct
     3181 ccaaaaggcc gcgacatact ttgcgaagcg cctgaaatga agtatgtctt tattgaaaaa
     3241 catcaggctg agttcagcat caaggcaatg tgccgcgtgc tccgggtggc ccgcagcggc
     3301 tggtatacgt ggtgtcagcg gcggacagga ataagccctc gtcagcagtt ccgtcagcac
     3361 tgcgacagcg ttgtcctcgc tgcggccttt acccggtcaa aacagcgtta cggtgcacca
     3421 cgcctgacgg atgaactgcg tgctcagggt taccacttta acgtaaaaac cgtggcggca
     3481 agcctgcgcc gtcaggggct gagggcaaag gcctcccgg
//
LOCUS       DAETPZ010000209         3395 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.215, whole genome shotgun sequence.
ACCESSION   DAETPZ010000209 DAETPZ010000000
VERSION     DAETPZ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.215"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..111
                     /locus_tag="K0W59_004202"
     CDS             <1..111
                     /locus_tag="K0W59_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="with Hns involved in transcriptional regulation of
                     hemolysin; non-specific DNA-binding protein which affects
                     the production of multiple proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI3024951.1"
                     /translation="NSERESFNSAADHRLAELITGKLYDRIPKEIWKYVR"
     gene            150..740
                     /locus_tag="K0W59_004203"
     CDS             150..740
                     /locus_tag="K0W59_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBI3024952.1"
                     /translation="MKLIIFILIVLIIATLLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            980..1240
                     /gene="repA"
                     /locus_tag="K0W59_004204"
     CDS             980..1240
                     /gene="repA"
                     /locus_tag="K0W59_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HBI3024953.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1464..1538
                     /gene="tap"
                     /locus_tag="K0W59_004205"
     CDS             1464..1538
                     /gene="tap"
                     /locus_tag="K0W59_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HBI3024954.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1531..2388
                     /gene="repA"
                     /locus_tag="K0W59_004206"
     CDS             1531..2388
                     /gene="repA"
                     /locus_tag="K0W59_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HBI3024955.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3088..3279
                     /locus_tag="K0W59_004207"
     CDS             3088..3279
                     /locus_tag="K0W59_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024956.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          913 a          839 c          851 g          792 t
ORIGIN      
        1 aacagtgaac gtgaatcatt taattcagca gcagaccacc ggctagcgga attaatcacc
       61 ggaaagttat atgacaggat accaaaagag atatggaaat atgtaaggta ataattcagc
      121 aataattaat tgccaggagc agataaataa tgaagttgat tatttttatt ctcattgttc
      181 tgattattgc aacgctactt atccggatta tcctcagaag tgtaaatcag cattctcctt
      241 tgctgatgca attgcatgcg gcaggtatac ggactggtga tgcagagcga atactgtcca
      301 gcggtgaata ctggcaacgt cagaagacgc tgctgacaga aagggaagtc agttttatga
      361 aaggactgtt cagaattgtg gatatgaagc ggtggtatct gtgtccgcag gtacgggtcg
      421 cggatatcgt ccagctgaac gggaatatcc ggccacgatc acgccagtgg tggcagttat
      481 tcaggatggt gtctcagtgg catgttgatg tggttatcgt tgagctgcgt tcgttcagta
      541 ttgttgcagc agtagagctg gatgatgcca gccatttacg accggaacgc agacgccggg
      601 atattcttct ggaagaggtt ctgaggcagg ctggtattcc gttgctcaga agccacgatg
      661 ccagaaaact gctgcagatg accggagaat ggctgaatac aacaggggct gatcagcagt
      721 caccggaaca tcgtagctga cgccttcgcg ttgctcagtt gtccaacccc ggaaacggga
      781 aaaagcaagt tttcctcgct cccggcgttt ccataactga aaaccatact atttcacagt
      841 ttaaatcaca ttaaacgaca gtaatccccg ttgatttgtg cgccaacaca gatcttcgcc
      901 acaattctca agtcgctgat ttcaaaaaac tgtagtatcc tctgcgaaac gatccctgtt
      961 tgagtattga ggaggcgaga tgtcgcagac agaaaatgca gtgacttcct cattgagtca
     1021 aaagcggttt gtgcgcagag gtaagcctat gactgactct gagaaacaaa tggccgctgt
     1081 tgcaagaaaa cgtcttacac acaaagagat aaaagttttt gtcaaaaatc ctctgaaaga
     1141 tctcatggtt gagtactgcg agagagaggg gataacacag gctcagttcg ttgagaaaat
     1201 catcaaagat gaactgcaga gactggatat actaaagtaa agactttact ttgtggcgta
     1261 gcatgctaga ttactgatcg tttaaggaat tttatggctg gccacgccat aaggtggcag
     1321 ggaactggtt ctgatgtgga tgtacaggag ccagaaaagc aaaaaccccg ataatcttct
     1381 ttaactttgg cgagtcagaa agattaccgg ggcccactta aaccgtatag ccaacaatca
     1441 agctatgcgg ggagtatagt tatatgcccg gaaaagttca agacttcttt ctgtgctcgc
     1501 tccttctgcg cattgtaagt gcaggatggt gtgactgatc ttcaacaaac ttattaccgc
     1561 caggtaaaga acccgaatcc ggtgttcaca ccccgtgaag gtgcccggac gcttccgttc
     1621 tgcgggaaac tgatggaaaa ggcggtgggc ttcacctccc gttttgattt cgccattcat
     1681 gtggcacatg cccgttcgct tggtctgcgt cggcgcatgc caccggtact acgtcgccgg
     1741 gccattgatg cgctgctgca ggggctgtgt tttcactatg acccactggc caaccgcgtc
     1801 cagtgttcca tcaccacgct ggccattgag tgcggactgg cgacggagtc tgctgccgga
     1861 aaactctcca tcacccgggc cacccgggcc ctgacgttcc ttgcagagct gggactgatt
     1921 acctaccaga cggaatatga cccgcttatc gggtgctaca ttccgaccga tatcacgttc
     1981 acatctgcac tgtttgctgc cctcgatgta tcagaggagg cagtggccgc cgcgcgccgc
     2041 agtcgtgtgg aatgggaaaa cagacagcgc aaaaagcagg ggctggatac cctgggtatg
     2101 gatgaactga tggcgaaagc ctggcgtttt gttcgtgagc gtttccgcag ttaccagaca
     2161 gagcttaagt cccgtggaat gaagcgtgcc cgtgcgcgtc gtgatgcgga caggcagcgt
     2221 caggatattg tcaccctggt gaaacggcaa ctgacgcgtg aaatctcgga agggcgcttc
     2281 actgccagtc gtgaggcggt aaaacgtgaa gtggagcgtc gtgtgaagga gcgcatgatt
     2341 ctgtcacgta accgtaatta cagccggctg gccacagctt ccccctgaaa gtgacctcct
     2401 cagaataatc cggcacgcgc cggaggcatt cgcacgcctg aagcccgcca gtgcacaaaa
     2461 aatcagcgct acatacaaaa aacaacctca tcatccacct tctggtgcat ccggtccccc
     2521 ctgtttttga tacaaaacac gccccacaga cggggaattt tgcttatcca cattaaactg
     2581 caagggactt catcgtaagg ttacaaccgt tcacgtcata aagcgccagc cgtcaaccct
     2641 acagggtgca atgtatcttt taaacacctg tttatatctc ctttaaacta cttaattaca
     2701 ttcatttaaa aagaaaacct gttcactgcc tgtcctgtgg acagacagat atgcacctcc
     2761 caccgcaagc ggcaggcccc aaccgaagcc ggtttagtta caacatgcaa acataaaccc
     2821 ctaaaaaacc caagtccaac gcagaactga aaccacaacg cccctcctca taactgaaaa
     2881 gcgccgccgc cccgcccgaa gggcgggaac agagtcgctt ttaattatga atgttgtaac
     2941 tacatcatca tcgctgtcag tcttctgcct ggacgtatcg agtacacgct cgtaagcggc
     3001 cctcacggcc cgctaacgcg gagatacgcc ccgacttcgg gtaaaaccct cgtcgggacc
     3061 actccgaccg cgcacagaag ctctatcatg gctgaaaacg ggtatggctt agcagggctg
     3121 gggatgggta aggtgaaatc tgtcaatcag taccggctga cgccgggctt cggcggtttt
     3181 actccggtat cacatgtaac cacagcgtgt cgccttccat gccgctggcg cggcatcaga
     3241 ataatacaag cagcatttaa tgcatttgca aaagtgtgag taatattaag tggtcgtttc
     3301 agggatatcg ttaagacttg agcgatagga attaaaaccc caaaaagatt aaaaaaacac
     3361 cacaaaacgg atgtttcttc aacaccactt ttgct
//
LOCUS       DAETPZ010000210         3391 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.216, whole genome shotgun sequence.
ACCESSION   DAETPZ010000210 DAETPZ010000000
VERSION     DAETPZ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.216"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(188..883)
                     /locus_tag="K0W59_004208"
     CDS             complement(188..883)
                     /locus_tag="K0W59_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HBI3024957.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(876..2303)
                     /locus_tag="K0W59_004209"
     CDS             complement(876..2303)
                     /locus_tag="K0W59_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HBI3024958.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2314..3033)
                     /locus_tag="K0W59_004210"
     CDS             complement(2314..3033)
                     /locus_tag="K0W59_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HBI3024959.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT          849 a          945 c          709 g          888 t
ORIGIN      
        1 aagggaaccc gttttcagga atggatgcct gtctttttca ctgtaaatat cctggcatta
       61 ctaatgcctg gatatttaca gaagagcagc ataaccatga cggaacgggg ttaagtcagg
      121 ttgtccttaa cgctattttc aacctcgtat gccttcttca ggtttatgtt cagatctcat
      181 atctctctca gcaatcctca ataatcagat acaccgcttt caccggacca tgaaccccga
      241 cgactttgat aagctcaata tccgccgttg agctgggacc actgatgatg ttaatacatg
      301 acggcattcg ttcaccggcc tgcgctttct gatgcaattt ttctgcgagt tgcgctacac
      361 gcggcaggat tgtgctttta cgcaggataa aaagagaaga ttccgggagc aggctcaatg
      421 aacgcccgcg ctcggcggcg gaaaaaagaa ccacgcctcc cgattcggtt aaaccatatt
      481 cagcatacac gacacccact ttagcctgct ctgcctgcga gatattctcg gcacctttcg
      541 ccggatccca aacaacggca ttgcattcct gctgcaaacg ttcactaatc cccaattcct
      601 ccagcctcgt gtcaccgcta atcacgaccg actgatctcc cagctcttta cacagacgta
      661 ttgcagcttc tgccgccttc gcctcgctgg tcagctcaca gcgcgtcaac ataacatcgc
      721 tggcaaactg aataaacgcg tcacagcgct gctgttggtt aagttgggta agccgctcgt
      781 tagcatagtt gttaagcggc gcatcttctg cttgcggttc aagtcgcagc gggcgaccca
      841 gtgcctgagc aacgttattc aaaaattcgc tccgattatc cattcttttt ctcctgcgcc
      901 tgatgtttct taaaccaact acggaaactc tctccgtcag cttcaggaag atcgcgtgct
      961 tccatccagt cgctaatcgc gccaaatttg agtggtgttt taccgccatt gataaaccag
     1021 cttgccgcat gagcgccggc catcatcccg actttccaca atcctggatg actattggca
     1081 taagcgaaca tttttatcgc ccgttgctct gcttttgcgg tgatcccttt ttcagccatc
     1141 acccgacgat gacgcaaaat cagttttgac agcggaatac gcaccggaca cacgctgtca
     1201 caagcagtgc ataaagagca ggcgtagggt aaatctttaa aatctttata gccgccaagt
     1261 agcggagaaa tcaccgcacc aattggccct ggataaatag agccatatcc atgaccgcca
     1321 atatggcgat atgccggaca agtattcata caagccccgc agcgaataca gcgcagcaca
     1381 tcccgaaatt cagaggccag cacctcagaa cgcccgttat cgacaataac cagatgaaac
     1441 tcttcaggac catcaacgtg ccccgcttcg cgcggccctg tcagccaggt gttgtatccc
     1501 gtcaaacgtg caccaacggc actgcgcgcc agcatggtga tcaatacatc tacctcggca
     1561 aacgtggggg caatacgctc cattcccatc actgcaatat gcgttttagg cagcgtggta
     1621 cacattcgcg cattaccttc attggtcacc aggcataccg aaccggtctc tgccaccgcg
     1681 aaattacagc cggtaatacc tatttcagca ctgaggaaat cttcacggat tttttgccgg
     1741 atgaataagg tcatcgcttc aggcgtttcc ggcccctcat agcccagacg ttcgtgtagc
     1801 actcgacgga tctgatggcg atctttatga attgccggga ccacaacatg agatggcgga
     1861 tcttgatcca gctggagaat atattcaccc agatcggttt caatcacctg aatgccagca
     1921 tcctgcaaca catgattgac accaatctct tcggtcacca tcgatttaga tttcaccacc
     1981 ttccgggcat ttttgcgttg ggcaacctgt aaaatgtagc gggtagcgtc ttctttggtt
     2041 tttgcaaaat agacgtgacc gccgttttgc gtcacttttt ctgagagctg gtacagataa
     2101 gcgtcgagat tactcaaaac atgatcacgt atctgggcgg cccgatcgcg ccactcctcc
     2161 cagtgcccca attcatcgac cattttttgc cgatttgccc caatacgctg ctgcgcgttt
     2221 gccaccgctt tgcgcatgat cggatcttca atttgctgac ggatgcgtgt cttaaaatct
     2281 gtattactgg ttttgatcga catctttata tcctcagcgg ctcatcaaca cttcagcaat
     2341 atgcatcact ttgactttct gcccttcccg ttgtaatcgc ccactgatgt tcagcaggca
     2401 actcacgtca gcaccaatta aatactcagg gcggacttcc atcaggtgca caaccttttc
     2461 tttcaccatc tcgccggata tttcggccat tttgaccgag aacgtgccgc caaatccgca
     2521 gcaggtatcc tgttcagcaa aggtcaacag ctccagtcca cgcacatttt tcagcagcgt
     2581 aagtggctcg tccttcactc ccagcttacg ggccaggcta caagatgggt gatacacagc
     2641 tctcccttgc aaactggcac ctacatcgac tacccctaat ttattaacaa taaaagaggt
     2701 gagatcctgc atacgcgcgg caaccttttc ggcacgtgat gcccattcag gttcatccgc
     2761 cagatacgtc gggtaacttt ttacggcata ggtgcaagag ccagccggtg aaataatggg
     2821 atcgtcgtta tcctccagtg cggcgatcag atttttcatc cctggaatcg cttctttgat
     2881 ataaccgcta ttgatcgcag gctgaccgca gcatccctgt ttctccggga aatttacgcg
     2941 acagccgagt ttttccagta gcagcacgga gtctcgtgcc attcttgatt tcagggcgtc
     3001 accaatacag gtgacaaaga aattgacatt cacaactaat actccattac ttcatgccca
     3061 tttatgcccg atgtttcccc cccaccgggg gatcaatcat caataaatat ggcgttcttt
     3121 ttaaattggc tagcaacata ccacccctca tttgtatgac aaatgataat ctttcatcca
     3181 aacttcaaat gcaataacta attttatttc tttcggcatc gctcacattt tatggggaaa
     3241 tcgacgatca tttttggcat aagaaatcca tttaaaaatg gctacatccc ttatacctat
     3301 tcagagctac ttattcaggg gttattgaga tatacactga tggtgtacaa aaaaatcacc
     3361 gacaataatc acgtatcaag taatagtcgg a
//
LOCUS       DAETPZ010000211         3355 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.217, whole genome shotgun sequence.
ACCESSION   DAETPZ010000211 DAETPZ010000000
VERSION     DAETPZ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.217"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            107..418
                     /locus_tag="K0W59_004211"
     CDS             107..418
                     /locus_tag="K0W59_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005086658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HBI3024960.1"
                     /translation="MDAHWKTVLKMSVKRWLWLIIVSVLMFATTGSLLWCQGMKINAN
                     MNILREQKESLEKLNAKTWGVRYNEDSNGRFLVLPEGMKAETNWTMENGKQNAVRLVQ
                     E"
     gene            425..658
                     /locus_tag="K0W59_004212"
     CDS             425..658
                     /locus_tag="K0W59_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HBI3024961.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(1237..2355)
                     /locus_tag="K0W59_004213"
     CDS             complement(1237..2355)
                     /locus_tag="K0W59_004213"
                     /inference="COORDINATES: protein motif:HMM:NF013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBI3024962.1"
                     /translation="MNSEIKVMSNRDPSIDTLRVTACIFVIISHYGYIFSQTRLSFIP
                     SFYGGVLGQVGVSLFFFISGYLAFISLDRKNTIDYFLDKGVRILTPYIITYFFISLLL
                     ILLSYLNKEIINTVPLVSIMTTGGQYTRIIPVFFALDGILNAHFSYDFLFLTGEWFIG
                     CVIMLYVISPIVFHVMNTFTFKRNIFFLFVITITTFPISNISSSFLNFPFWFFACRLP
                     EFMVGMLAFKYKNKLQKNTTVILSVVICILTVLLIGPNEFKTNIVNHQLSIIPLMCIS
                     IIIYKAALIINKKFNLYFFNSYAKCIYISMLIQHVVIYRLTSIIEINNASVFGYLFIL
                     LMVFFLTFYLSKKILIPTSKIESILIKKMNIINAKKAN"
     gene            complement(2473..2562)
                     /locus_tag="K0W59_004214"
     CDS             complement(2473..2562)
                     /locus_tag="K0W59_004214"
                     /inference="COORDINATES: protein motif:HMM:NF015365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024963.1"
                     /translation="MRQHLTRKGQKSLSLSTSVEQHDKAISTI"
     gene            complement(2998..3159)
                     /locus_tag="K0W59_004215"
     CDS             complement(2998..3159)
                     /locus_tag="K0W59_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBI3024964.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNGFRGVSL
                     EVMRLLRLN"
BASE COUNT         1018 a          635 c          773 g          929 t
ORIGIN      
        1 cagggaactg acctcaaacg aatctttaat caggaacgct atcaacgacc atacatcagc
       61 cctgaaagaa cttctggaga ggtatcagaa aacgaccgtc gatacgatgg acgcacactg
      121 gaaaaccgta ctgaaaatga gcgtgaaacg ctggctgtgg ctgattatcg tctcagtact
      181 gatgtttgcc acgaccggga gcctgctgtg gtgccagggc atgaagataa acgcgaacat
      241 gaatattctc cgcgagcaga aagagagtct ggagaagctg aacgcgaaga catggggcgt
      301 gcgttataac gaggacagca acggacgttt tctggtgttg cccgagggga tgaaagccga
      361 aacgaactgg acgatggaaa acgggaaaca gaatgccgta aggctggtac aggagtaaca
      421 gagcatgacg gagctggaaa gacatttgct gaacgcatta gagcaactgc aacaggacta
      481 tatgcagcgg ctgaacgaat gggagagcgc cttcgtggaa ttgcagaaaa tgttttcgct
      541 tacgcaacgg gacaacgcga tgctgaacga gcgggtcatg cagttgagtc agcaggtgca
      601 gcacttgagc gagcagacag aacgcttgag ccagttatac agcgagaaca ggagataagg
      661 gacgaacggc tgatacagga atgcgaacat gcgttatccc tggagcgtga gcgacagctg
      721 gaaatacagg aacggacact ggatggccct tcgctgggat ggtaatcaga gacaggctaa
      781 aatgttgtca gcgcgtttcc ggtgcatttt ttgggataat ggttggtttg ggggcaccgg
      841 gaacgcgctt cagtcagacc ttgctgaaaa cataggaacg cgttaaaacg catacgggcg
      901 cgattctggt gtgtcttttg ttgctgtgtt ttgcctctat tgccagatcg cgctcattcc
      961 cctgaacttc tgttttcccg ctcaggtccc cgtcttcttc gcttccgaaa gcatgaaaaa
     1021 aatccgtttc agctgtctct ggctggcaat tcctgcgtga ttcacatggc tgcatagcta
     1081 tgcatgtggt taaaatttac cgggtgcgat cgcggcagtt tttcgggtgg tttgttgcca
     1141 tttttacctg tctgctgccg tgatcgcgcc gaacgcgttt cagcggtgcg tacaatgaga
     1201 tgttatggta aattcaagtc ttagatttta tgatgtttaa ttagcttttt ttgcgtttat
     1261 aatgttcatc ttttttatta aaattgattc aatctttgat gtgggtatta atattttctt
     1321 tgacaaatag aaagttaaaa aaaacaccat taatagtata aataaatacc caaaaacaga
     1381 agcgttatta atttcaatta ttgatgttaa tctgtatatt actacatgct gaatcaacat
     1441 ggatatgtag atgcatttag catatgagtt gaaaaaatag aggttaaatt tcttattaat
     1501 tattaatgct gctttgtaaa tgataatgct tatgcacatt aaaggtatta ttgataactg
     1561 gtgattgact atgtttgttt tgaactcatt aggccctatt aataatactg tcaatataca
     1621 tataacaacc gatagaataa ctgtggtgtt tttttgtaat ttatttttgt atttaaatgc
     1681 aagcatccca accataaact caggaagtcg gcaagcaaaa aaccaaaatg ggaagtttaa
     1741 aaatgatgat gagatatttg atatcgggaa tgttgtaatt gttatcacaa ataaaaaaaa
     1801 tatgttacgt ttaaatgtaa aggtattcat aacgtgaaaa actatcggtg atatcacgta
     1861 cagcataata acgcacccaa taaaccactc gcctgtaagg aaaagaaaat cataagagaa
     1921 atgggcgttt aatataccat caagggcaaa aaatactgga attattctag tgtactggcc
     1981 tcctgttgtc attattgata cgagagggac tgtattaatt atctctttat ttaaataaga
     2041 caataaaatt aagagtaaag agataaaaaa gtaagtgata atataaggag taaggatcct
     2101 tactcctttg tcaaggaaat aatcaatggt gttctttcta tctagtgaaa taaatgccaa
     2161 atatccagat ataaaaaaaa acaagctcac acctacctgg ccaagaaccc ctccataaaa
     2221 tgatggtatg aaagatagcc tggtttggct aaatatgtag ccataatgag atataataac
     2281 aaaaatgcag gctgttactc gcaatgtatc aattgacgga tctctattag acataacctt
     2341 tatttcgctg ttcaaattat cgcctttagt tgatgatgta aaattaacgc tataaataag
     2401 ctacacattc tagagcagcg aaaggatgat tcaagtattt ttaataataa aacgcattga
     2461 taatgtttat gttcagatgg tgctgatagc tttgtcatgc tgttccactg atgttgataa
     2521 cgacagagac ttttgtcctt ttcttgttag atgctgcctc agattcggat tatggcgtta
     2581 aaggggtttg acgctcaacc gaacggtttc acacgttaag agctttttgt ctgataaatc
     2641 aaaatataat ggacattgaa aaacgccctc aaaacggtgt ctttttaatc gaagattggc
     2701 acaaaacgga caccaccgtc cattagtcga ttttttagac ggcttttctc gcctaacgtg
     2761 agttttcgtt cggttgagca tcagacccct tttatattgt agatttctat ttttagacag
     2821 ttggaacagg acaattaaca acagatctac ttgagatcat ttgcttgtcg tcgtagtctc
     2881 tttcccagaa acgaaaaaac cgccctgcaa ggcggtttct cgaaggttct cagagcgacc
     2941 aactctttga accaaggtaa ctggcttatg aggagcgcag taaccaaatc tgtcctttca
     3001 gtttagcctt aacaggcgca taacttcaag actaactcct ctaaatccgt taccaatggc
     3061 taccgccact ggcgatttgt cgtgtctttc cgggttggac tcaagttgat agttaccgga
     3121 acgggcgtgg tagtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac
     3181 tgcctacccg gaactgagtg tcaggcgtgg aatgagataa acgcggccat aacagcggaa
     3241 tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagcca ccagggggaa
     3301 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcg
//
LOCUS       DAETPZ010000212         3239 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.218, whole genome shotgun sequence.
ACCESSION   DAETPZ010000212 DAETPZ010000000
VERSION     DAETPZ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.218"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..328)
                     /locus_tag="K0W59_004216"
     CDS             complement(<1..328)
                     /locus_tag="K0W59_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBI3024965.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENI"
     gene            574..1335
                     /locus_tag="K0W59_004217"
     CDS             574..1335
                     /locus_tag="K0W59_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBI3024966.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(1442..1942)
                     /locus_tag="K0W59_004218"
     CDS             complement(1442..1942)
                     /locus_tag="K0W59_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024967.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(2093..3239)
                     /locus_tag="K0W59_004219"
                     /pseudo
     CDS             complement(2093..3239)
                     /locus_tag="K0W59_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          973 a          725 c          579 g          962 t
ORIGIN      
        1 ttatgttttc actgatgtaa aagcctactt tgggtatcgt aagaatcaca ttttcttcaa
       61 caccaatatt tttaaaactt ttgcgtaaca gggatatata ttggttcaat gtgttgtttg
      121 acagatcaaa tgaaaatctc tccaaaacgc tatcaatgat tgagttcctg tcaaccgctc
      181 caccattact ttccagtaac actgccaaaa tacaccccgc cgtagctggc aaatttatct
      241 catcctgggt atttacattc gagagcgagc atttgttcgg atcaaaaata atcgcgtcat
      301 tgataatgaa aaaacatctc cgctccatgc aggcaacaac acaaaataag attttgtcat
      361 tttagcctat tcctggtgga actgacacaa gaaactaaaa tacaaatata accctgaaaa
      421 agtcatataa aacttatatc cacagaataa aaaaccaaac atgtcagttt tatcaatata
      481 aagtatcgca aggcgaaaag tgatgcaaga atactcctaa gtaaatgaaa gcgcaccaca
      541 tgaaattatc aacacatgta atttcatcat taggtgctat cgaaaataac tcaggagcta
      601 cttaagatga ataatgtctt actcatgaga cccgatgact cagaatttat gatacctgat
      661 gactggtcag ggctaaacgc aaaaggcatt acatacgaac aagataatag agtgacagag
      721 tcttttctgc cttttaaatc tttttattgt tcaccggtgt gcacgacagt tgaactcatc
      781 tcacaaaata attgttttgc tatgggtatc cgctaccttc tggaaaagaa aagagactta
      841 aggctaaatc actttaaagc cttctctcat aaatgcgaga caaaacctga cgtgttaatt
      901 atgaccgggt gcaccgtcgc tgatacttat agtatattct cacgcattgt tgaagaaaat
      961 ataatgaaaa cgatgacacc tatcgtcatc attactgggc atcactcaga agatctttgt
     1021 tctctggcat cgaaactggg aggagttaaa ataatatcac cgacagaaaa gattgcaaaa
     1081 atagaaaacg ctatcaatga tgcttttaac agcaagccta aaaaaataag atttttaaca
     1141 gatatactct ctcctcaaca aattgagacc atgaaaatgg ttgcgcaggg tagatccgtt
     1201 aatgagattt cagccgcaat gaatattagc atcaagaccg tgtatgcaca taaagttcgt
     1261 gctatcaata agctgggaat taagaaaaaa gtttatgaag cgctttttta taaagcgcta
     1321 attcagttca actgatgtaa aaatcaccct cgcttaataa ataatatatt ctatgtcttt
     1381 ttttataagt taaaaaaggc gggatatcaa atcccgccat gaaattaact aatcatctgc
     1441 attattctgc gtagatataa gcctgggtac ggatggtgta agagtctggc tcaacaacct
     1501 gttctttcat atgggaaacg acgcgatagg caccacctgt tccagtattt ttcagcatca
     1561 tataacgctt accgccaatc tcttcggagg cagctgaagg aggatcctgc tcaagcgttg
     1621 tcagggaaac tttcagttta tggtccgggt tattgtcacc agaaattact gcatccgctg
     1681 aagtactgct ctccgcataa atatcatccg ggaaagttac agcatagtcg tggctctgcc
     1741 catcagatgc gccaacaata cctgttgcca aaggcgtcca tgcagttaac gtaccgctat
     1801 ataccccttt cactggttga atggtattat taacaacgat aggcttggtc aaagtaagat
     1861 tagctgacgc ttcagtaccc tggcttacta aagttcccgc ctgagaagca aaagctgtcc
     1921 ccgccagcat gattgctaac aatgttttct tcattatatt ttctccatga ttttcattta
     1981 cactgcaaaa tgcagtttct ttctttgcat ttacgtgcta tcgcgaaaat gcttccacca
     2041 aataatggaa acatcttcac aaaaataaat tatgagtttc ttaatttaga aactataaac
     2101 atgtcgccgc tatctggtat aaccctgatg cacttttctt cgcaggaagt tggtattttg
     2161 cgatacagga ttgatcttta gaataacccc atttcacatt aaactcgcct tccgcactca
     2221 agccagaaac ataagcaata ccatcttcac ctacaatgcc ggccatattt tcagtgtctt
     2281 tgctataact aatcacagcg ccgaagggga cgggtttacc ctctttctgt tttaaggtta
     2341 ctaatccttt atccccgctc ttcactgcca atgtcgcagg cacaacggct ccagctgtcg
     2401 gtacaaccaa ttgactagtt tgtggtaagt cgtaattctt acctatcgtt ttttcatcca
     2461 gaataacatc gtctcttcgg tatggctgca ctggcagtat tgcataccca cgaaaatcag
     2521 tactcagtcc cggctagcca ttaacgttaa cccccgttgc tccgggtgcc tctataaggg
     2581 ctaatgtccc atccacattt tgaccaaatg taagtccatc ccttgtaata agtatcccac
     2641 cgcttaaatt tgcacctagc tgtttcatgt tttcgctgga gctgtaatgg ctactaaact
     2701 gaccatatga tccatttttg ccaacttgca caatgttcat cgtgctcttc ggttgtgact
     2761 gatgagcgtt agttatattt gtcgctacat cccaccatac accaccacta ttctgacctg
     2821 ataatgaaac ctgatagtta ttaccctgct ctcccgaacg actcatctgt gaagacgcat
     2881 aaatttgatt ttcgtcaccc agcgataatg gcactgaaat acccacgctt aatttccgat
     2941 ctactttcca tttatgcgat gtcagcaagt cgttttttgc ccagttaatg gagaatgcta
     3001 tgcctctcca taccgtacta taactcagtc tggtagaccg cgtgtttcct gtgccccggg
     3061 aagaattata cctcgtggta ttgaagttca aattcccgat attaccaagg ggctgggata
     3121 aggatatcgt agagccatcg tgactattct tatctctttt tctgttccgt tcatcctgtg
     3181 cacttactgc ttcagtcagt gtggcataac ctgatgcata acggttcgtg gttatagac
//
LOCUS       DAETPZ010000213         3156 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.219, whole genome shotgun sequence.
ACCESSION   DAETPZ010000213 DAETPZ010000000
VERSION     DAETPZ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.219"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            238..2106
                     /gene="kup"
                     /locus_tag="K0W59_004220"
     CDS             238..2106
                     /gene="kup"
                     /locus_tag="K0W59_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HBI3024968.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2273..2692
                     /gene="rbsD"
                     /locus_tag="K0W59_004221"
     CDS             2273..2692
                     /gene="rbsD"
                     /locus_tag="K0W59_004221"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBI3024969.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2700..3080
                     /locus_tag="K0W59_004222"
     CDS             2700..3080
                     /locus_tag="K0W59_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBI3024970.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYVEVMPVS"
BASE COUNT          708 a          756 c          830 g          862 t
ORIGIN      
        1 gaagggcctt cccgagagtg gtaaaggaga ggccctggaa ataccccaca gccccaaaaa
       61 agagccagtc agttgcttaa ctgactggca ttactcaaag ggggggcttt ttctttgagc
      121 caggttaatg tggccgcatt taggagtacg attttgccgt taatcgtgca tactgtgcgc
      181 ttttttgtgg accaagggac taagcacaca tttcatattt caacgaaaga ctagtctatg
      241 agcactgata ataagcaatc attgcccgcg attaccctcg cggcgattgg agttgtctac
      301 ggcgatattg gtaccagccc gttatataca cttcgtgaat gtttgtccgg ccagtttggt
      361 tttggcgttg aacgcgatgc cgtgtttggc tttttatcgc tgatcttctg gctgctaatc
      421 tttgtggttt ccattaaata tctcaccttc gtgatgcggg cagataacgc cggtgagggg
      481 gggatcctga cgttgatgtc gcttgccggg cgtaatacgt cggcgcgaac cacatcaatg
      541 ctggtgatta tggggctaat cggcggcagc tttttctatg gtgaagtcgt cataacaccc
      601 gctatttcag tgatgtcagc cattgaaggt ctggaaatcg tcgccccgca gctggatacc
      661 tggatagttc ccctctcaat tatcgttctc actttattat ttatgattca aaagcatggc
      721 acggctatgg tcggtaagct gtttgcgccg atcatgctga cctggttttt gattctggca
      781 ggactggggt tacgtagcat tattgctaac ccggaagtgc tgcatgcgct gaatccgatg
      841 tgggcggtgc atttcttcct tgaatacaaa acggtttctt ttattgcatt aggggcagtg
      901 gtgctgtcga ttacgggggt cgaggcgctg tatgctgata tggggcactt tggtaagttc
      961 cctattcgcc tggcgtggtt caccgtcgta ttaccttcct tgacccttaa ttacttcggt
     1021 cagggagcgt tgttgttaaa gaacccggaa gcgattaaga acccgttctt cctgttggca
     1081 ccggactggg cgctgatccc actgctgatc atcgccgcac tggcgacggt aattgcctcg
     1141 caggcggtta tctctggcgt cttctcattg acgcgtcagg cggtacgtct gggatatttg
     1201 tcgccgatgc gcattattca cacctccgaa atggagtcag ggcaaatcta tattccgttt
     1261 gtgaactgga tgctctatgt cgcggtcgtg attgtgattg tcagctttga gcactccagc
     1321 aacctggcgg cggcgtacgg gattgcggtg accggaacca tggtactgac gtctattctc
     1381 tcgactaccg tggcacgtca gaactggcac tggaataagt attttgttgc gctgatcctg
     1441 attgctttcc tttgtgtcga tattccattg ttcaccgcta acctcgataa actgctctcc
     1501 ggcggctggt tgccattgag cctcggtact gtgatgttta tcgtgatgac cacctggaag
     1561 agcgagcgtt tccgcttgct gcggcggatg catgaacatg gtaactctct ggaagcgatg
     1621 attgcttcgc tggagaaatc accgcctgtt cgcgtgcccg ggaccgcggt gtatatgtcg
     1681 cgtgcaatca acgtcattcc ctttgcgctg atgcataacc ttaaacataa caaggtattg
     1741 catgagcggg tgattttgtt aactctgcgc accgaagacg cgccatatgt ccataacgtc
     1801 cgtcgggtac agattgaaca actgtcgccc actttctggc gcgtggtggc aagttatggt
     1861 tggcgagaaa cgccaaacgt ggaagaagtt ttccaccgct gcggtctgga aggattaagt
     1921 tgccggatga tggaaacctc cttctttatg tcgcatgagt cgttgatcct cggcaaacgc
     1981 ccgtggtatt tgcgtctgcg cggcaagctg tacttgctgc tgcaacgtaa tgcgctgcgt
     2041 gcaccagatc aatttgaaat cccgccaaac agggttatcg aactgggtac tcaggtcgaa
     2101 atctaacgcc agacgcctcc tttcttcata agggggcgtt tttgttttca tggttaatca
     2161 ccatgtaaaa cgtttcgagg ttgatcacat ttccgtaacg tcacgatggt tttcccaact
     2221 cagtcaggat taagctgcgg atcagcgaaa cgtttcgctg atggagaaaa aaatgaaaaa
     2281 aggcaccgtt cttaattctg atatttcatc ggtgatctcc cgtctgggac ataccgatac
     2341 gctggtggtg tgtgatgctg gtttacccat ccccaaaagt acaacgcgta tcgatatggc
     2401 attaacccaa ggtgtacctt cttttatgca ggtgctgggc gtcgtcacaa atgaaatgca
     2461 ggtcgaggct gccattatcg cggaagagat caaacaacat aatccgcaac tccacgaaac
     2521 gttgctcact caccttgagc agctgcaaaa acaccaggga aataccattg aaattcgtta
     2581 caccacgcat gaacaattca aacaacaaac cgcagaaagt caggcggtaa ttcgcagcgg
     2641 agaatgttct ccgtatgcga atatcattct ctgtgctggc gtgacgttct gaggccgtca
     2701 tggaagcatt acttcagctt aaaggcatcg ataaagcctt cccgggtgta aaagccctct
     2761 cgggcgcagc gttaaatgtc tatccgggcc gtgtgatggc gctggtgggc gaaaacggcg
     2821 cgggtaaatc caccatgatg aaagtgctta ctggtatcta tactcgcgat gccggcacgc
     2881 ttttatggct ggggaaagaa acgacattta ccgggccgaa atcttcccag gaagccggga
     2941 ttgggattat ccatcaggag ctgaacctga tcccgcagtt gaccattgcc gaaaacattt
     3001 tcctcggtcg tgagtttgtt aatcgctttg gcaaaattga ctggaaaacc atgtatgtcg
     3061 aagtaatgcc agtcagttaa gcaactgact ggctcttttt cggggctgtg gggtatttcc
     3121 agggcctctc ctttaccact ctcgggaagg cccttc
//
LOCUS       DAETPZ010000214         3142 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.220, whole genome shotgun sequence.
ACCESSION   DAETPZ010000214 DAETPZ010000000
VERSION     DAETPZ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.220"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..740)
                     /gene="ipaH9.8"
                     /locus_tag="K0W59_004223"
     CDS             complement(<1..740)
                     /gene="ipaH9.8"
                     /locus_tag="K0W59_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HBI3024971.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGPRIYFSMSDGQ"
     gene            complement(1002..1097)
                     /locus_tag="K0W59_004224"
                     /pseudo
     CDS             complement(1002..1097)
                     /locus_tag="K0W59_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1133..1438)
                     /gene="ccdB"
                     /locus_tag="K0W59_004225"
     CDS             complement(1133..1438)
                     /gene="ccdB"
                     /locus_tag="K0W59_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HBI3024972.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1440..1658)
                     /gene="ccdA"
                     /locus_tag="K0W59_004226"
     CDS             complement(1440..1658)
                     /gene="ccdA"
                     /locus_tag="K0W59_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HBI3024973.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2194..3142)
                     /locus_tag="K0W59_004227"
                     /pseudo
     CDS             complement(2194..3142)
                     /locus_tag="K0W59_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          781 a          762 c          761 g          838 t
ORIGIN      
        1 tgttgtccgt cactcatgga gaagtaaatc cgtgggccgt ggtagtccgg cgaagaggtt
       61 aatctttgca gggcttgcag agcatgggat gataatgggt tatcactaat atgtattgaa
      121 cattcattcc tcagattaag aatgctttcc gggatatgac ttatctgatt tctatcaaaa
      181 aaatactctc tgacaacggg attatttccc tcagaaaatg ctggtagctc agtgaggaaa
      241 ttacgggtcg ctctgagatt tttaagagcc tgtgggagcg atggtaagga gactatttcg
      301 ttatagctga tattcatagt caataacgaa tcgggtaaag ctggcaggtt ttccagctca
      361 ttgtgttgta cctgaagtga ctccagcgca ggaggtagca cgggcaattc tgataattta
      421 ttgctggcgg aatataattt ttttagcgta acaggcagtt caggtaggtt agttaattga
      481 ttatatgata cattgagcag cgttatctga gctggtaagt tgtcaggtag cgaggacaga
      541 tttaaacggt ccagtcgaag ttcatcggaa ttattgataa gacattcttt aagtcgggag
      601 acagcctcat cacgctcttc accggggagc gcttgttttt cccatttatc ccatgctgaa
      661 aagtaatcag catatgtacc ggaaatagtg ttataaaaag aattttgggg caatgaaaag
      721 ttattattta tcggtaacat gatgggcatc cagttaaagc cggtggacaa atggctttgt
      781 taaaagaaat aagtaggagt ttttatgaag ttcattccat gaaatgaact tcagcacttt
      841 tttattataa taaggataaa caaaattggc tgtaaaaaaa cgatactgtt tcagttaacc
      901 gtgaatggcc tgtttgcacc gtattgttct tgtttttcgg ttagttaaac atcgtacttc
      961 atatttgaac ggtaagcggc tcgtcagaac cgtattgata tttactgaga ggtcagatca
     1021 actttccagg gcaacagatc gcgtacccgg tttgccggcc agtcctggat atgctcaatg
     1081 acgtaacgca gccattttgt caacgccacg atttttgacc ctagcctgac atttatattc
     1141 cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca
     1201 cttcttcccc gataacgaag atcggcacac tggccatatc agtggtcatc atgcgccagc
     1261 tttcatcccc gacatgcacc accgggtaaa gctcacggga gactttatct gacagcaggc
     1321 gggcactggc cagggggatc accatccgtc gtccgggcgt gtcaataatg tcactctgta
     1381 catccacaaa cagacgataa cgactctctc ttttataggc gtaaacctta aactgcattt
     1441 caccagtccc tattctcatc ggcaaaagag ccgttcattt caataaaacg ggcgatctca
     1501 gccatcccct gctgattttc agcctgccag cgttccgcac gcagacggcg ggcctctttt
     1561 tgcatggccg tatttaccag gcctgagata ttcacgtttg cggatttaag taactgatag
     1621 ctgtcactgt ctatagtgac agtaatacgc tgcttcatga taagccctca aaaacatact
     1681 tatgatatgt atatgagtat atattaatgg tacaaaccat caatatccat aaaaagtaca
     1741 ccattaattc tctataagtt tttaatacaa ttttttctgc tgcgattttt ttatactata
     1801 attagcatac ttataacacc gtaagtaaat tacatgaaag caggcacagc gtgatccgat
     1861 ttttctgcgt ataacgattt tccggaagtg taatacggtt gcgccccggg ccgggcaccc
     1921 ttttctgttg ttgtgtacgg gcccacagag aatggtaccg gaggcgggct cagaagtact
     1981 gagtcgggtg atcgcgtcag gccgcgaagt actgacacgg gtattggtga ctggtgatga
     2041 agtactgccc cgggtatgat ttcaggctga ttcaggttct gattctctgt cagtccgcgt
     2101 ggttgctggc ctggctgatt tcctgttgtc aggcgttcaa caaccgactg caggttatag
     2161 cccggttcac gaacacctga cggcaggtat cactcacccg gtgaaggtaa atccctcaag
     2221 aggcagcaac tctgccagtc gctcctccgg ccattccggc agacgcgtca ggacgtccgt
     2281 cagccaggca tgtggctcca gaccattacg ttttgctgtt tccagaaggc tcattatctg
     2341 tgccgcccgg tttccggcca tcagtgaacc ggcgaacagc cacgctttcc gtcccataac
     2401 aaccgggcga atggcacgct cgcagatatt gttgtccagg ggcaccgctc cgtcacccag
     2461 gaagcagctc agttcatcgc gccggttcag aatatagtta atcgccttat ttaatggccc
     2521 gtcgggcgga cagcacggct cctgctcttc aagccaggca aacaggtcat tcactatcgg
     2581 ccgggagtac cgttatcgcc actgccgtat tttttccaca gggcgctcac ggatgagttt
     2641 ctcaatgcgg taaagacccg cgatttttct cagctccacg cctgcacggg gctccctgct
     2701 ggccttatac agatttgcga agccgcgccc tgcgtgactc cagcaacagg ctgatgtgat
     2761 gccggggtga ttatccgcca gagatttgta cacgctgtat ccgtcactca ccaacgtacc
     2821 gccacaccag cactgcagcc aggtctcagg gtatctgagg gcccgtccgg tctgcgagtc
     2881 aaaacagacg accggcggac cgcttctttc accgctgacg taagcacaca gccagccgga
     2941 gcaggattta ccgcctttcc gggtatcaag gagcctcagg gacgtttcat ctgcatgaat
     3001 aacatcccgt gtcagaagct catggcgtag cagtttcgcc agcggggcca gtgcagctcc
     3061 ggcaacaccg accatatccg ccattgtact gacggggagc tccacaccca ttcgggagaa
     3121 aatgtgctgc tggcgataca gg
//
LOCUS       DAETPZ010000215         3134 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.221, whole genome shotgun sequence.
ACCESSION   DAETPZ010000215 DAETPZ010000000
VERSION     DAETPZ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.221"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            804..998
                     /locus_tag="K0W59_004228"
     CDS             804..998
                     /locus_tag="K0W59_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBI3024974.1"
                     /translation="MNKQQQTALNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHEL
                     AEELQNSIQIRFEAESETGT"
     gene            complement(2023..3132)
                     /gene="fepE"
                     /locus_tag="K0W59_004229"
     CDS             complement(2023..3132)
                     /gene="fepE"
                     /locus_tag="K0W59_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HBI3024975.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
BASE COUNT          928 a          772 c          653 g          781 t
ORIGIN      
        1 aaaaaaacac aatatattaa catatggttt ttttattcta tcatatgtta ggaaaaaacc
       61 taaaaaccat tcagcagaca tttaacataa cattttattg cgcgcaccgc taaaacgcgc
      121 tgaacgcgat tctgccgcaa acaccgcaaa aagagatgtt ttccccgggg taaaccggaa
      181 aaatgcgtca ggatcgcttt cagctcactg catagctatg catgaaagtg aatgacgatc
      241 ggtttgggcg catacagtgc ccatgagaca cattttcgga cattttcagt tcgtgaatcc
      301 ctgactcatt gctacatatc tgaacagaca cccctagcag cgcccccagg ggcgtcctgt
      361 aaaaaaacac accgcgccgc tagcagcccc ccccctaaaa agcggtgttg gcgcagctac
      421 aacaccgcgc cgacaccgct tttttaaata tcacaaagag agcaagaaga actaatttgt
      481 cataactctc tatttataaa gaaaaatcag aaaaaacttg tttttgcgtg gggtgtgggc
      541 atttcaggca tgagaacaga ctcgcgtttt tccggagtat cagcggaatt attgattaag
      601 aaacactcga cgcagagcga aattttatgc aacagcggct gcactgcgca gggattattt
      661 atcagttgat atgctgtgcc atgatacgtc ctaaagaaac aggacaggat gtgcaattgt
      721 aataccgtcg cccgcaacgt cttacaatta ccacctgctc agggcccccc ccgacacccg
      781 gaactgacgg agaacaacgg aaggtgaaca aacaacaaca aactgcgctg aatatggcgc
      841 gatgtatcaa aagccagagc ctgacactgc ttgaaaaact ggatgctctg gatgccgacg
      901 aacaggcggc catgtgcaaa cgactgcacg aactcgcaga agaactccag aacagcatcc
      961 agattcgctt tgaggccgaa agtgaaacag gaacataacg aagctgacgg aggcggtcac
     1021 ggctttcccg tgggcgtttt cgtcagcaga caagcccgtc agggcgcgtc agggggtttt
     1081 agcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt atatactggc
     1141 ttaatcatgc ggcggcagag cagatggtga gagaacagcg acatatgagg tgtgaaatgc
     1201 tgcacagata cgtaaggaga aaatacagct tccggcgctc ttccgcttcc tcgctcactg
     1261 actcgctgcg ctcggtcgtt cggctgcggc gagcggtgtc tgctcacgca aagacggata
     1321 tacggttatc cacagaatca ggggataaca ccagaaaaaa catgtgagca aaaaagaggc
     1381 cccgaaaaaa ggccgcgccg gtggcgtttt tccataggct ccgcccccct gacaagcatc
     1441 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggacttaaa gataccaggc
     1501 gtttccccct ggtggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt
     1561 cattccgctg ttatggccgc gttcgtctca ttccacgcct gacactcagt tccgggtagg
     1621 cagttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1681 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttaacg ccattggcag
     1741 cggccactgg taaacaggat gtgcgagaga tgtattgaga gttcttgaag tggcagcctt
     1801 actgaggcta cactagaagg acagatttgg tgacatgtct cgcacaagac tgttaccacg
     1861 gttcagaagt tccccaactt actgaacctt cgaaaaacca cctccccagg tggttttttc
     1921 gttttctaga gtagaagact acggctgtaa gggaatgatc tcaagaagat cctttgaact
     1981 ttttataaaa gttgcttata aaatgatgca tcagcacaac ttttactcaa tcacactcat
     2041 ctttctggcc tccatggcat gacgtaaaag tatatagcca catgcaagca tccctccaat
     2101 gacagaagaa agaattacaa ttatggcatt acttggccca tcctttctga ccggtaacgt
     2161 tgggctcaat tgatactgaa atggcataaa atcaacatcc cccactttcg ccacaaccaa
     2221 ttgattcaca acatattgac gatttcgcaa ctccccactc aattccgaaa catccttgat
     2281 tgattttttg atgttcaatt tagttgctat accatcagaa ccgagagcca caggaaaatc
     2341 tgggtcatcc ttcataatct gaccattgct gtatacaggt ttttttattc cagccgcatt
     2401 agcaacctcc agtgaataat tgaggcgtcg aatatctgca ttcagctggt ttctaatttt
     2461 tatctcatcc agtgcaagaa tttctttttc aacattagtt ttaacttcta gtttatttct
     2521 tatatcttcc atcaaatccc ttacaacaag ggaagaaaca taattaatat acccagacaa
     2581 cacatcgtga gcttctttac ttgttggagc agtaaaactc agagtccatg atacatacaa
     2641 tgatgacttg tcgtttttct tactggcatt atcatcaatc gacttcatat ttttactcaa
     2701 ctcaacaatt gcacgatgca gttcgagagc gctaatgtta ttctttttaa taatctccat
     2761 aacataagga gatgatctca tatactcatt caacgaatta acggactgaa atttcttaat
     2821 aaacagagca aatgcgctat cacgattgat atcaaaatcc actcccagaa catgaagctt
     2881 ggaaagctcc ttatttaatt cactccattg aacagactca gcaggagtaa ccacagcaga
     2941 actagtccat ttttgtggca aaacaaaaga aacaagaaag ccaatacaag caaaaaatag
     3001 aatcacccca acaattttta tttttgctgc ccataaaacg ctaatcaagc caaaaaggtc
     3061 aatttcttca ttaccattcg gcaacatgtt gtaattagaa aaatgttcgt cagagacctt
     3121 tgagattttc attg
//
LOCUS       DAETPZ010000216         3039 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.222, whole genome shotgun sequence.
ACCESSION   DAETPZ010000216 DAETPZ010000000
VERSION     DAETPZ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.222"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            54..1085
                     /gene="ynaI"
                     /locus_tag="K0W59_004230"
     CDS             54..1085
                     /gene="ynaI"
                     /locus_tag="K0W59_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HBI3024976.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRERKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(1136..2749)
                     /gene="mppA"
                     /locus_tag="K0W59_004231"
     CDS             complement(1136..2749)
                     /gene="mppA"
                     /locus_tag="K0W59_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HBI3024977.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKGRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
BASE COUNT          790 a          708 c          672 g          869 t
ORIGIN      
        1 ccgccagggg tttccggcat gacgacaatt atctggagga gcctgataaa acaatgatcg
       61 ctgaactgtt tacaaataat gcgcttaatc tggtcattat tttcggtagc tgcgcagcat
      121 tgattctgat gagcttttgg tttcgccgtg gaaatcgtga aagaaaagga tttttattcc
      181 atgcggtgca atttttaatc tacaccataa ttatcagtgc tgttggtagc atcattaatt
      241 atgttataga aaactataaa ctcaaattta tcaccccagg cgttatcgat tttatctgta
      301 cgtccctgat tgcggttatt ctgacgatta agctatttct gctgattaat cagtttgaaa
      361 aacagcagat taaaaaaggt cgcgatatca ccagtgcgcg gattatgtcg cgcattatca
      421 aaatcaccat tattgtagtg cttgttctgc tttatggcga acatttcggc atgagccttt
      481 ctggcttgct gacctttggt ggtattggtg gtctggctgt cggtatggcc ggtaaagata
      541 ttctgagtaa tttcttttcc gggatcatgc tctatttcga ccgtcctttc agtattggcg
      601 actggatccg ttcaccggac agaaatatcg aaggtacagt agcggaaatt ggctggcgaa
      661 ttaccaaaat tacgaccttt gataatcgtc cattgtacgt accgaactcg ctgttttcgt
      721 cgatcagcgt agaaaaccca ggacgaatga ccaaccgccg cattaccacg accattggtt
      781 tacgttatga ggatgcggca aaagtgggcg ttattgtcga agccgtacgt gagatgctga
      841 aaaatcaccc ggccatcgac cagcgacaaa ccttactggt ttatttcaac cagtttgctg
      901 actcgtcatt gaatattatg gtttattgct ttaccaaaac cacggtatgg gctgagtggc
      961 ttgctgcaca gcaagacgtt tatttgaaga ttatcgatat tgtacagtca cacggcgcgg
     1021 actttgcctt ccccagccag acgctgtata tggataacat tacaccgccg gaacagggtc
     1081 gctaaatcag acatcactgg cgttatccac aagggtaacg ccagttccca tcacatcaat
     1141 gcttcacaat atacatagtc cgactgtacg ctacatcttc agggttatta atgggataac
     1201 ctttcagcca cggtttgatt aatcgtccat tggtatattg ataaattggt gcaatcggtg
     1261 cttgctccat gaggattttt tctgccgcgt tgtaatcggc attacgcgct ttaacggtat
     1321 tttccgtcga tgcctgggcc agaactttgt cgtatgccgg attgttaaag cgtgaaatat
     1381 ttcctgaatg cattgacgtt aataatgtca ggaaagtgga gggttcatta taatccccca
     1441 cccatgaggc gcggataaca tcaaaattgc cggtgttacg gctatcgata taggttttcc
     1501 attcctgatt ttgcaattta acatctacgc caaggttctt tttccacatc gatgctacag
     1561 caatcgcaat tttttgatgg ttttctgaag tgttatacaa aagcgtcagc ttcagcggtt
     1621 tttgcggacc ataaccagct gcgctcaaca aagtttttgc ctgcgcattc agttcttcct
     1681 gactcatttg ttcaaacggc gaaggttccg gcgtaaatcc ggcggtaaca tctggtataa
     1741 aatgccacgc tggcttttcg cccgtcccca acactttttc ggtcatcagg cggcgatcta
     1801 tcgtcatact taatgccagg cgaacgcgct gatctgccgt cggccctttt tgcgtgttaa
     1861 aggcataata ataggtcccg agctgcggtg gcgtataaac ctgccccgga atatccttca
     1921 acagcttctg atacatattt ttcgggaagg attcggtgat atcaatatcc cccgcgaggt
     1981 aacgcttagt ggctgcggat tcctgattaa ttggcaggaa ggtcactttt tgcagcaccg
     2041 ttttggcgtt atcccaataa tgggtattcg gtaccacgac cagtttttca ttgactacgc
     2101 gccctttaag aacataagcg ccattgccga tcagatttcc gggtttcgtc cactctttac
     2161 cgctttctac gttggctttt tgcaccggga agaaggcaaa gttagcggtt aaattcacaa
     2221 accacggcaa cggtttatca agctgaattt tcaaagtatg ggcatcaact gcggtgacgc
     2281 caagctgatc aggcgtagct ttaccatcaa taatcgcctg tgcgttgttg attcccgcca
     2341 gcgcggcaaa ccatgcaaat ggcgacaatg tttttgggtc caccagacgt tgccagctgt
     2401 agacaaaatc ttgcgccgtt accggtgtgc catccgccca ttttgcgtta tcgcgcaggg
     2461 taaaagtcca gatacggttg tcattacttt tccattgagt cgcgacgccg gggacaatct
     2521 cccctttttc gttctgattc accagacctt caaacagatc gcgaatgacc tgaatctctg
     2581 gcaggcccac ggctttagcg ggatccagcg acgcaggctc atctttaatg tggcgcacca
     2641 gctcctgctt ctctgccagt actgtgccgc tcggaacttc tgcagcatac gaaagagaaa
     2701 tgctgctgac caacagcgca caacacgtaa ctgaaacaga gtgcttcata agtttccctc
     2761 taaccgacat taaaattgat gcgtaattat ttgtttcgcc ttaaagaaat gcaaacaact
     2821 tccatcaaaa aagtgacaaa ggaagcatca aatgttttaa gtatcaatat atcaaatcag
     2881 atttagtgta aataatagac gccaaaatgc ggcaaagcac atagaacacc gaacactacg
     2941 ttcgggcttc gcacgttcaa accagttttc ggtatcataa tcagcatctg tcgccattgt
     3001 acgaagaata aaaaataacc aaaacgttga aaggaactg
//
LOCUS       DAETPZ010000217         2993 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.223, whole genome shotgun sequence.
ACCESSION   DAETPZ010000217 DAETPZ010000000
VERSION     DAETPZ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.223"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            44..358
                     /locus_tag="K0W59_004232"
     CDS             44..358
                     /locus_tag="K0W59_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3024978.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            640..1206
                     /gene="ipgB2"
                     /locus_tag="K0W59_004233"
     CDS             640..1206
                     /gene="ipgB2"
                     /locus_tag="K0W59_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HBI3024979.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(1468..1590)
                     /locus_tag="K0W59_004234"
                     /pseudo
     CDS             complement(1468..1590)
                     /locus_tag="K0W59_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1733..2287
                     /locus_tag="K0W59_004235"
                     /pseudo
     CDS             1733..2287
                     /locus_tag="K0W59_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            2627..2993
                     /locus_tag="K0W59_004236"
                     /pseudo
     CDS             2627..2993
                     /locus_tag="K0W59_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          849 a          648 c          681 g          815 t
ORIGIN      
        1 tttatagaag ctgtcagcgg ctacgtccca gccaaatcac ttcatgctcc agcgccagag
       61 aggcaaggtt gggtttgctc agctgccggt tgattttgtt gcgattgaac cggacagcgt
      121 ccagggcgtc ggcaaaagag cgaatctcac caatcgttgt ttcattatcc gcattaacga
      181 tagcttcaag aaacgccaag gttttatcga attcatcagc aacagtggct ccgggcatac
      241 cgtgaccgtt tatacaaaac ggcgttttca gcgtggagtt ttcatgaata gcctcaacac
      301 gaatgtcgtg aagccagtga tgtatcggct acgatcctca gctgatgcca gaccatagga
      361 ctgacactac gaacagcaat tttaatgacg taaatcttca taactacaac taacagggca
      421 tcattatgaa tgatgccggt tattttaccg ctgttggtcc cttcatttag ggactgcccc
      481 aagttttttc atactctctt tctcagagag tttagtctct gggattccag acggtccatc
      541 attgagccaa gggagtaaag aactcaaagc atttacagaa ttgtttgaat cagctaaaat
      601 aatgacaatg ttactatttt acatgacaaa gtgattataa tgcttggaac atcttttaat
      661 aattttggaa tcagtttatc tcacaaaaga tatttttcag gaaaggtgga tgagattatc
      721 cgctgtacaa tggggaaaag gatagtaaaa atatcatcaa ccaaaataaa tactagtatt
      781 ttgagctctg tttcagaaca aatcggtgaa aatattacag attggaaaaa tgatgaaaaa
      841 aaagtctacg tatcccgggt ggtgaaccaa tgtattgata agttttgtgc agaacacagc
      901 cgaaaaatcg gcgataatct ccgtaaacag atatttaaac aggttgagaa agattatcgc
      961 atttctctag acatcaatgc tgcacaaagt tccataaatc atcttgtatc aggaagcagt
     1021 tatttcaaaa agaaaatgga cgaactttgt gaagggatga atagaagcgt taaaaatgat
     1081 acaacaagca atgttgcaaa tcttatttcc gatcagtttt ttgagaaaaa tgtgcaatat
     1141 attgatctaa agaaattacg tgggaatatg agtgactata ttacaaattt agaatcgcct
     1201 ttctgatgaa agaacatccc gagtaaggaa actaagaaaa gtaaataccc aggaattgcc
     1261 tgagcggtgc tatatttctc ctcgcaagta agtcctaata tattgaattg ttgtatagcc
     1321 agtttttgta ttttcctagc tcatggatgt gggatccctc tcccctttct ctgctcaaag
     1381 gtaagcggct cgtcagaatc atattgatat ttactgagaa gtcagatcaa ctttccaagg
     1441 caacagatcg cgtacccggt ttgccggtca gtcctggata tgttcaataa cgtaacgcag
     1501 ccatttttct ggctccacat tgttcagacg gcatgtgccg atcagcgaga acaacaccgc
     1561 tgcatgttcg tcaccgctgt cggaacctgc gagtcgagtc agccggagag agtttcactc
     1621 tccggctgct tacggaaccg tacgtgacac tctcgcgtca tacggctctt gatcactcat
     1681 tcgatgcacg tagtaaatac cagtgagcaa attgatctta cccagcaata cggtcagtta
     1741 ccgcgcacat ggcctgcctg tgtcagaaaa tctgctggag caggattttt acgccagtgg
     1801 tccgaaccag aagtgggcgg gagacatcac gtacttacgt acagatgaag gctggctgta
     1861 tctggctgtg gtcattgacc tgtggtcacg tgccgttatt ggctggtcca tgtcaccacg
     1921 cctgacggcg caactggcct gcgatgccct gcagatggcg ctgtggcggc gtaagagacc
     1981 ccggaacgtt atcgttcact cggaccgagg aagtcagtac tgttcagcag attatcaggc
     2041 gctgctgaag tggcataatc tgcgtggcag tatgagcgca aagggctgct gctacgataa
     2101 tgcctgcgtg gaaagcttct ttcattcgct gaaagtggaa tgtctccacg gagaacactt
     2161 tatcagccgg gaaataatgc gggcaacagt gtttaattat atcgaatgtg attacaatcg
     2221 ctggcgacgg cacagttggt gtggcggcct cagtccggaa caatttgaaa accagaacct
     2281 cgcttaggcc tgtgtccata ttacgtgggt aggatcactt acgccctgtg gttgaaacgt
     2341 gagcatattg ttttcagatg ctgttgtctt gcttcagagt gatctgtcct ccggatttac
     2401 gcaaagttat ccacaggcac tgtgcattta aaaaacttat cttttcttct tgattttaaa
     2461 aactcagatc gtcatatcat tatgataaac ataataaaaa actctaaaca tgagaaggtt
     2521 tttaacgtaa ggtgagagga tttttaagtg tatccataga ttaaaaatga caatgcctcc
     2581 aattagttga attaggggtt ataatacccg cttttaaggt tctttcatgg tcacagttaa
     2641 ctttcattgc ccccgttgcc agtccgttca ggtttaccgt cacgggcaga acccaaaagg
     2701 acatgatcga ttccgttgcc gtgactgtca ccgggtgttt cagctcactt actgttacga
     2761 ggcccgtaaa ccgggagtta aaaaccaaat cactgaaatg gctttcaacg gtgccggagt
     2821 tcgcgatact gcccgtacgc tgaaaatcgg catcaatacc gtcatacgga cattaaaaag
     2881 ctcccgcccc ggcggataac atctgcgcca gtcgctcacg ctgatgttgc ccttatctgt
     2941 gaacttgatg ggcagtggag cttcgttggc agcaaggcgc aacaacactg tca
//
LOCUS       DAETPZ010000218         2844 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.224, whole genome shotgun sequence.
ACCESSION   DAETPZ010000218 DAETPZ010000000
VERSION     DAETPZ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.224"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(572..784)
                     /locus_tag="K0W59_004237"
     CDS             complement(572..784)
                     /locus_tag="K0W59_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBI3024980.1"
                     /translation="MVVWRFGGQSTGYKDSDRLNYIEIGTWYYFNKNMNIYTAYQINL
                     LDKSDYVLAHGLNTDDQLAVGIVYQF"
     gene            844..2072
                     /locus_tag="K0W59_004238"
     CDS             join(844..1159,1159..2072)
                     /locus_tag="K0W59_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI3024981.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWKDGRRSRHSDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRIYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDIMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTAVRS
                     PESNGIAESFVKTIKRDYISAMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQRASNGLSDNRCLEI"
     gene            complement(2081..2844)
                     /locus_tag="K0W59_004239"
                     /pseudo
     CDS             complement(2081..2844)
                     /locus_tag="K0W59_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          757 a          622 c          740 g          725 t
ORIGIN      
        1 agttagggta gtcactatat ttgcgactga gctggtcgct atttttaacc aggctgaaaa
       61 attgagtcaa tatatcgaaa gcggtagttg gcgggcataa gtatgtaatg atgattctgc
      121 actggtgttg aatattattc acgccaaact ttcctcattg agcaatgtcg tcgcaagtgg
      181 cctcaatgat tttagaccat tatcagaggt ccggagcggc gggaagagat ttattgctgt
      241 ggaataattg tggtactgat gattatttat atcccggatt ggtagggaaa agcttttttc
      301 atgcactatt tttgcaaata tttctatatg ggaagcggtt ttgttacaga ttaaatttat
      361 aaagaaatgc atttggggtg ttgggttgaa gagtagctgg tagatgttat tgtgccagca
      421 ggggcagggg aatgtgccgt atcgatatgc tttggcaatt gcgtgtgccg cagatggaga
      481 tgaaatgcca ataaaaaagc ctgagttcga tgagggacga actcaggctg gtgttatgtt
      541 ttacagtaac acgcctcggc gcgttggctg attagaactg gtagacgata ccaactgcca
      601 gttggtcgtc ggtgttcagt ccatgagcga gaacataatc actcttatca agcaggttga
      661 tctgataagc ggtatatatg ttcatattct tattgaagta ataccaggta ccgatttcaa
      721 tatagttcag acgatcagaa tctttgtaac ctgtagactg gcccccgaat ctccagacaa
      781 ccaatatcac ttatttaagt gatagtctta atactagttt ttagactagt cattggagag
      841 cagatgattg atgtcttagg accggagaaa cgcagacggc gtactacaca ggaaaagatc
      901 gctattgttc agcagagttt tgaaccggga atgacggtct cccttgttgc ccggcaacac
      961 ggtgtggcag ccagccagtt atttctctgg cgcaagcaat accaggaggg aagtcttact
     1021 gctgtggccg caggagaaca ggtcgttcct gcctctgaac ttgctgctgc catgaagcag
     1081 attaaagaac tccagcgcct gctcggcaaa aaaacgatgg aaaatgaact ccttaaagaa
     1141 gccgttgaat atgggcgtgc aaaaaagtgg atagcgcacg cgcccttatt gcccggggat
     1201 ggggagtaag cttagtcagc cgttgtctcc gggtgtcgcg tgcgcagttg cacgtcattc
     1261 tcagacgaac cgatgactgg aaagatggtc gccgcagccg tcactcagat gatacggatg
     1321 tgcttctccg tatacaccat gttatcggag agctgcccac atatggttat cgtcgggtat
     1381 gggcgctgct tcgcagacag gccgaacttg atggtatgcc tgcgatcaat gccaaacgta
     1441 tttaccggat catgcgccag aatgcgctgt tgcttgagcg aaaacccgct gtaccgccat
     1501 cgaaacgggc acataccggc agagtggctg tgaaagaaag taatcagcga tggtgctctg
     1561 acgggttcga gttccgctgt gataacggag aaaaactgcg agtcacgttc gcgctggact
     1621 gctgtgaccg tgaggcactg cactgggcgg tcactacggg cggcttcaac agtgaaacag
     1681 tacaggacat catgctggga gcggtggaac gccgcttcgg caacgagctt ccggcgtctc
     1741 cagtagagtg gctgacggat aatggttcat gctaccgggc taatgaaaca cggcagtttg
     1801 cccggatgtt ggggcttgaa ccgaagagca cggcggtgcg gagtccggag agtaacggca
     1861 tagcagagag cttcgtgaaa acgataaagc gtgactacat cagtgccatg cccaaaccag
     1921 acgggttaac ggcagcaaag aaccttgcag aggcgttcga gcattataac gaatggcatc
     1981 cgcatagtgc actgggttat cgctcgccac gggaatatct acggcagcgg gccagtaatg
     2041 ggttaagtga taacaggtgt ctggaaatat aggggcaaat ccaacctgct acgtccagtg
     2101 ctttagaata ggagtaaccg atggatggac gcagaccaaa gtcgaactga tattgcagaa
     2161 cagcttcgaa gttttgtgtt ttgttcacaa catcaccgtc gtcggaattc atgttgtgag
     2221 attcaccgta catcacagca gcataaacat tgttagcgtc atattttgtt gacaacgccc
     2281 aaacttcggc tctttcgcct ttaccatcac cagactgcca atcagtacgg tcagagtttg
     2341 cataagcacc agcaaatgag aaaccgtcaa tagtgtaagt tgcagataaa ccatggccat
     2401 cgccatttgc tttgtagttg cctgtacctt catttttacc ctgatattgc agagcaaaat
     2461 ttagaccttc aaccatgcca aagaaatctg tgttacgata ggttgcaaca ccgttggtac
     2521 ggttggtcat gaacaggtca gtaccagccc atgagtcacc gccccactca acaaacatat
     2581 cagtaaatgc ttctgcatca tacgcgacac cgacattacg actgtagtcg aatgaaccaa
     2641 aatctttgta actcaaacca gcatatgcca aacgagtttg gtcagggttg tgtctgtttg
     2701 atgcttccag gtctagttca aactgaccgt aaccagtcat ttcagggttg atctgagttt
     2761 cacctttcac gcctacacgg gcataagagg tatcttgaga attatcgcct ttttcaccat
     2821 tttcacggtc ggtcagaatg cgct
//
LOCUS       DAETPZ010000219         2789 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.225, whole genome shotgun sequence.
ACCESSION   DAETPZ010000219 DAETPZ010000000
VERSION     DAETPZ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.225"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..985
                     /gene="yeeE"
                     /locus_tag="K0W59_004240"
     CDS             <1..985
                     /gene="yeeE"
                     /locus_tag="K0W59_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HBI3024982.1"
                     /translation="LTGGFRDMYIAKNNRMFYALLIAISAQSVGVFALIQAGLLTYEA
                     GAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRAGEGLIGSWIALFTYMVMSAVMRSPH
                     ASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVLLVITLWVVMKELKKPKLKVATLPPR
                     RTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATGRMFGLGVTSPTANILQFLVAG
                     DVKYINWGVFLVLGIFVGAFIAAKASREFRVRAADAQTTLRSGLGGVLMGFGASIAGG
                     CSIGNGLVMTAMMTWQGWIGLVFMILGVWTASWLVYVRPQRKARLATAAAN"
     gene            999..1226
                     /locus_tag="K0W59_004241"
     CDS             999..1226
                     /locus_tag="K0W59_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HBI3024983.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1269..2693)
                     /gene="sbcB"
                     /locus_tag="K0W59_004242"
     CDS             complement(1269..2693)
                     /gene="sbcB"
                     /locus_tag="K0W59_004242"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HBI3024984.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWQEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
BASE COUNT          643 a          660 c          742 g          744 t
ORIGIN      
        1 cctgactggt ggttttcgcg atatgtatat cgcgaaaaat aatcgcatgt tttacgcctt
       61 gctgattgca atttcggcac aaagcgtggg ggtttttgcg ttaattcagg cgggcctact
      121 gacttacgaa gccggggcgt tcccgtggct tggtactgtt ataggtgggt atcttttcgg
      181 gctgggaatt gttctggcgg gtggatgtgc caccgggacc tggtatcgcg cgggtgaggg
      241 attgatcggc agttggatcg cgcttttcac ttatatggtg atgagtgcgg tgatgcgttc
      301 tccacacgcc agtggtttaa atcaaacctt gcagcactac actactgaac ataactctat
      361 tgctgagact tttaatttgt ctgtgtggcc gttggttacc gttttgctgg tgataacgct
      421 ctgggtggtg atgaaagagt tgaagaagcc aaaactcaaa gtcgcgacct taccgccgcg
      481 ccgaaccggg atcgctcata ttctgtttga aaaacgctgg catccctttg tcactgctgt
      541 actcatcggt ttgattgcgc ttttagcctg gcccctgagt gaagcaaccg gacgcatgtt
      601 tgggttagga gtcacttccc caacggccaa tattctgcaa tttctggtcg cgggtgacgt
      661 gaaatacatt aactggggcg ttttcctggt gttagggatc ttcgtggggg catttattgc
      721 agccaaagcg agccgtgagt tccgcgttcg cgcagctgat gcacaaacaa cattacgtag
      781 cgggttaggt ggtgtactga tgggcttcgg tgccagtatt gcaggtggtt gctctatcgg
      841 taatggactg gttatgactg caatgatgac ctggcagggc tggattggcc ttgtatttat
      901 gattctcgga gtctggactg cgtcctggct tgtgtatgtt cgaccgcagc gtaaggcgcg
      961 actggcaact gctgcggcaa attaagtaag gtgtaaagat ggcgattaaa aagctggatg
     1021 tggtgacgca ggtctgtccg tttccactca tagaagcaaa agcagcactg gcagaaatgg
     1081 ccagcggcga tgagttggta attgagtttg attgtaccca ggcaactgaa gcaatcccac
     1141 agtgggcggc agaagaagga catgccatca ccgattatca gcagattggc gatgcagcgt
     1201 ggagcatcac cgttcaaaaa gcctgactca acattgtcct ccgccgtacc agcggcggcg
     1261 gcttcaaatt agacgatctc ttccgcgtac tgccaaagtg cttttaacag cgccactttc
     1321 tctttgtcat cggcatattg ttgtaccagc atctgcaatt catcagcata accctgcaaa
     1381 aactctggcg tgaagacctg gtggcggtgc tcctgccagc gttgctgctc ggcataatcc
     1441 agcgtccccg ggaagttgcg tgcccgataa ttgaacaaca acttttcaat tcgtttatca
     1501 acaaaagtga tatccagtgc cggtaaatta cgcggctcgg tttccagcac aattttcatt
     1561 gctgcacgat cggcgtcact gaaaaagcca ttataaagct gtgcatccac attatctgaa
     1621 ggtgtaaacg gttcggcttc cgcgaatatc gctaccactt tttcacgcac ttgcggattt
     1681 tcacgcagaa tttttaggtt atcgaggcaa tgctgacgat taattcccag tcggtcggca
     1741 tcttccgggc gtagcgtatt cgcctgggcc agtaccggac atttattgat atgcaccagc
     1801 ttaaccggaa cggcggcgtt atcgccaaga tcggcttttg cggtatataa acgctcgcgc
     1861 aatgtgtcga tatccagctc cagtaatggc gaaatgtctc ctgccaaatc cgccataatt
     1921 acggcattgc gattttcagg atgccacgcc agcggtgcca cccagctggt attgccacgc
     1981 catgccccaa acattccgga aacgtgagcc aggggtttca tctgcggaac atcaatcaac
     2041 gccatcagtt tgtgtttatt acgatgggta aagagataat caaacaggcg tggctgacgc
     2101 gtttttacca gtttcgccat cgcaatagtg gcgtacacat cagccatcgc atcgtgggcg
     2161 ttgctatgtt caataccatt cgctttggtt aaatgctcaa ggcgaaagct cggtagaccg
     2221 tcatcatttt caggccagtt tattccttcc gggcgcaggg cataacaggc acgcataaca
     2281 tccagtaaat cccagcgcga gttatcatgc tgccagctcc aggcgtaagg atcgtagaaa
     2341 ttacgataaa aaacgttgcg tgtgacttcg tcgtcgaaac gcacattgtt gtagcccaga
     2401 atacaggtct tcggtacggt aaaaagcgag tgaatacggg cggcaaacgc ggcttcgttt
     2461 tctcctttcg cccgtgcctc ctgcggggta ataccggtaa ttaatactgc tccaggccgg
     2521 ggtaaatagt catccgcggg cttgcagtaa aagacttcgg gttcgccgat gacattgaat
     2581 tcgtcatcgg tgcgaatggc tgcgaactgt gcagggcgat ctaacgcggg gtgcgtgcca
     2641 aaggtttcgt aatcgtgaaa gaggaaggta ggttgtttat ctgtgtctgt cattgtctag
     2701 cattgtccac tgtagttaat taactgtctg ttataactcg aaaagcaacc cttttttcac
     2761 ctgagtatgt ctacgaacgt caaatatag
//
LOCUS       DAETPZ010000220         2648 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.226, whole genome shotgun sequence.
ACCESSION   DAETPZ010000220 DAETPZ010000000
VERSION     DAETPZ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.226"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..1475
                     /locus_tag="K0W59_004243"
     CDS             <1..1475
                     /locus_tag="K0W59_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBI3024985.1"
                     /translation="AIGLSIAAGGAALTWYQVAETDLSRTAAAAHEAVSVTERLLDEA
                     QGAIDLVYPLSVQPCTPQLRTELGRLTISHEHIRVINIFNQNLLSCSSWEGADPVREF
                     ISAGDNQTLILATDDYISPGVPVMILRKTDNDNAITASIATKWVADNMTLLNLRRPLS
                     LRVGNTVLTSDNTLVKAIPGGNRHAVYSGLYPFSIEYPGDLSVPLTLYFHDGALSLLL
                     SVLLGAAVATGLWKIKFRRKSRYDEIYEAMRKDEIVPWYQPIMDASTGEIAGVEVLAR
                     WVKADGEVIYPGSFITEVERSDLVIPLTRSLMARASRDLPPLTGDGKRWHISFNITQS
                     HIMETGFIAECLTFIDAFTPGSITLTIEPTEREPFDNSDEMRCQLKRIHDAGIALALD
                     DFGTGYANMEYLSEIPVDIIKIDRVFVNRIGQGESAEHLLTSLIEMANTLKKEIIAEG
                     VETEAQAAWLYERGVGKLQGFLYSPALPPDKLALFKPKMS"
BASE COUNT          682 a          558 c          618 g          790 t
ORIGIN      
        1 tggcgatcgg cctttcgatt gctgcgggtg gggccgcgct gacctggtat caggtcgctg
       61 aaacagacct gagcagaaca gccgctgctg cccatgaggc ggtctcggtt actgaacgac
      121 tgctggacga agcccagggc gcaatcgatc tggtttatcc cctgagtgtg caaccatgta
      181 ccccacagct cagaaccgaa cttggccggt tgaccattag tcacgaacat atccgggtga
      241 ttaacatttt taatcagaat cttttgtcct gctcgtcctg ggagggcgca gatcctgtgc
      301 gggagttcat ctccgctggc gacaaccaga cgctgattct ggcgacagac gattatatat
      361 cacctggtgt gccggtcatg attctacgca aaacggataa tgacaacgcc ataactgcga
      421 gtatcgccac taaatgggtg gctgacaata tgacacttct gaatcttcgt aggccgctgt
      481 cgctgcgcgt agggaatact gttcttacca gcgataacac gctggtgaaa gcgatacctg
      541 gagggaatag acacgcagtt tattctggcc tgtatccctt ttccatagaa tatcctggcg
      601 atctttccgt tcctctaacg ctttattttc atgatggtgc gttatcttta ctgctttctg
      661 tgcttctggg ggccgctgtg gcaaccggcc tgtggaaaat aaaatttcgc cgtaagagcc
      721 gctatgatga aatctacgag gcaatgagga aagatgagat tgtgccgtgg tatcagccaa
      781 ttatggatgc ctcaacaggt gaaatagctg gcgttgaagt acttgctcgg tgggtaaaag
      841 cagatgggga agtcatttat cccggcagtt ttatcactga agtagaacgt tcagatttag
      901 ttattccatt gactcgtagc ctgatggcgc gggcatcccg ggatttaccg cctctgacag
      961 gcgacggaaa gcgttggcat attagtttca atattacgca gtcacacatt atggaaacgg
     1021 gatttattgc cgagtgcctg acgtttattg atgcttttac accgggcagt atcactctca
     1081 ctatagagcc cacagagcga gaaccctttg ataacagcga tgagatgcga tgccagctta
     1141 agcgtattca tgatgcaggc attgcccttg cgctggacga ctttggcacc ggttacgcaa
     1201 atatggaata tcttagtgag atcccagtcg atatcatcaa aattgatcgc gtctttgtga
     1261 acagaatagg tcagggcgaa tctgcagaac acctgctgac cagccttatc gagatggcca
     1321 atacgctgaa gaaagagatt atcgccgaag gtgtggaaac cgaggcgcag gccgcgtggc
     1381 tatatgagcg tggtgttggc aagttacaag gattcctata ttcaccggca ttaccgcccg
     1441 ataagttggc actgtttaaa ccgaaaatgt cgtgagttca acggtacgcg ctatcggaat
     1501 cattgaacct gtgagggccg ccccctttat tactcagggg ggctttcaac gttctgaatt
     1561 aagatgtttt atgactcact gagcaggatg tgagttccaa cgtaatctcc gctattttta
     1621 ctgtttttca tttgctgcct catctatccc ttcgccttgc ctctgagttg cgttaaaggc
     1681 tgatgtactg cctttaaccc gttcaataca tagccttttt gtcaatcatc atcccgaaga
     1741 gcgttgcact gtgccttcca tagctgtggt tgtcctctca atctgtcagc ccatttatgg
     1801 aaaatcttaa cgaagcaaca atgagatatg taaaaaaagg gccgatttaa tcattcatta
     1861 agacaggtca ttttttctta ctgtgagggc ggtaagcttt tctgtcggta acaggttggg
     1921 agatgctggt attatgagat ttttctaccg tcctgcggcg gaatatgtga aaaatagaaa
     1981 tgattattat tattgcatgg ttatttttag tttgtgatgg tggagaataa tcctattccc
     2041 gtgaccttgt tttttacatt ttatttaact gcatggtata tgcattttgg acggtgaaag
     2101 caagaagcta ttaggcgcta taggtctttt gacggttttt gaatttcccc tcctgatatc
     2161 tctgttatat ttttgtgaat ttctccaatc caaatcaaag tcccaataag tcatggatgc
     2221 atggttaaaa taactatcat ttgtgtaacc tttggtgctt ataacttttc tttattcttt
     2281 tttcaggatt acctggaggg attgaccaaa aaaacattca ttgaatgata tttttctaat
     2341 taatgaggtt ttctaagctc gttgaaataa aaaatatatt ttaatcatgg atttgcatgc
     2401 gtttctttcg ctttttttac acaagttgac attgaactca gcaactataa gaatagccac
     2461 atcccagacg gtgaccgata aaggaaatac cgttaccata tttaaatgaa ttggaaaata
     2521 tcaccgattt gaagtcgact cgttcgggta attattaata ctgaatcttt aatgcttatt
     2581 tcttaagctt gttaacaacg ggaatgaata atgaaagtgt ccaaaattgc actggcaatg
     2641 ggtcttag
//
LOCUS       DAETPZ010000221         2624 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.227, whole genome shotgun sequence.
ACCESSION   DAETPZ010000221 DAETPZ010000000
VERSION     DAETPZ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.227"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..106)
                     /locus_tag="K0W59_004244"
     CDS             complement(<1..106)
                     /locus_tag="K0W59_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HBI3024986.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(147..602)
                     /gene="csgB"
                     /locus_tag="K0W59_004245"
     CDS             complement(147..602)
                     /gene="csgB"
                     /locus_tag="K0W59_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HBI3024987.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1356..2006
                     /gene="csgD"
                     /locus_tag="K0W59_004246"
     CDS             1356..2006
                     /gene="csgD"
                     /locus_tag="K0W59_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HBI3024988.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2011..2400
                     /gene="csgE"
                     /locus_tag="K0W59_004247"
     CDS             2011..2400
                     /gene="csgE"
                     /locus_tag="K0W59_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HBI3024989.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            2425..>2624
                     /locus_tag="K0W59_004248"
     CDS             2425..>2624
                     /locus_tag="K0W59_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="nucleator protein that participates in the
                     polymerization of curlin (CsgA) subunits into curli
                     (extracellular fibers from Escherichia and Salmonella spp.
                     that are involved in the colonization of inert surfaces
                     and biofilm formation); part of the curli secretion and
                     assembly protein complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgF"
                     /protein_id="HBI3024990.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSA"
BASE COUNT          768 a          553 c          498 g          805 t
ORIGIN      
        1 cgccaccacc gtggttaccg ccgccgccgt actgaggaac aacacctgcc agagcgctac
       61 cggagaatac gattgctgca attgctgcta cttttaaaag tttcatgtaa aacccccatc
      121 ggattgattt aaaagtcgaa tggtaattaa cgttgtgtca cgcgaattgc catttgcgac
      181 tgtctctgca ctacaattgc cgttttttga gtaccatact gtgtaatatt tgctttatta
      241 ccagaacctt tctggataat catcgcagta ttaccataag caccttgcga aatactggca
      301 tcgttggcac tgcccgcctg atcaatatat gcaaggttat aatctcctgt ctggtcaatc
      361 tttgcccgat tgctactacc ttcttgcgca acaaccgcca aaagttttga gcctccctgc
      421 cgtaactgag cactattatt agtcccagct tgaccaatta tggctgcctg attaaatgaa
      481 gacttactca attcatttac cgcgaagtta tattctgaat tagctaaatc ataacctgct
      541 gcggctgcaa tcccaggcgc acccagtatt gttaacatca taaataacaa tttgtttttc
      601 atgttgtcac cctggacctg gtcgtacatt taagaaatta aatcatttca acttggttgt
      661 taacgcaacc tgtattttgt taacgctgcg ttacgatgga aagtatgtct gcggaaatat
      721 ttttaataac tcacccgcgc gttgtatttt tctttttttg ttcataccaa agtattaaaa
      781 cccgcgtaaa tacgctgtta tctacgcaaa aaatattttg ttttctctta aatctccgtt
      841 ttccgctatc aaaaatcacc agacagtcat tcttcttgcc cgtcgctgat tgctgcgata
      901 tgtcttgcgc acaagccgtg acaaacctca tctttttgca gtaaaaaaat tgtccacgga
      961 ggtgtggaga aaaaacaaaa acgttttaca tgacgaaagg actacaccga aatatttttt
     1021 atatgtatta ttagtaagtt agcaccattt gtatgatttt ttaaaattgt gcaataaaaa
     1081 ccaaatgtac aacttttcta tcatttctaa acttaataaa accttaatgt taacatttta
     1141 atataatgag ttacattttg ttacatgttt aacacttgat ttaagatttg taatgcctag
     1201 attgaaatca gatgtaatcc attagtttta tattttaccc atttagggct gatttattac
     1261 tacacacagc agtgcaacat ctgtcagtac ttctggtgct tctattttag aggcagctgt
     1321 caggtgtgcg atcaataaaa aagcggggtt tcatcatgtt taatgaagtc catagtattc
     1381 atggtcatac attattgttg atcactaaac cttctttgca ggcgacagct ctcttgcagc
     1441 accttaaaca atcgctagca attacaggaa aattacataa tattcaacgt tctctggacg
     1501 atatctcttc aggctctatt attcttctgg atatgatgga agcggataaa aagcttatcc
     1561 attattggca ggatactttg agcaggaaaa acaacaatat caaaatattg ttgctaaata
     1621 cgcctgaaga ttacccgtac cgcgacattg aaaactggcc tcatatcaac ggcgtttttt
     1681 atgccatgga ggatcaagaa cgtgttgtca atgggttgca aggcgtcctg cgtggcgaat
     1741 gctactttac gcaaaagctt gccagttacc tgattacaca ttctggtaac tatcgttata
     1801 acagcacgga atcagccctc cttactcatc gggaaaaaga gatcctgaat aagctgcgta
     1861 tcggcgcgtc taataacgag atcgctcgtt cgttgttcat cagcgaaaat acggttaaaa
     1921 cgcatcttta taatcttttc aagaagatag ccgtaaaaaa ccggacacaa gcggtttcct
     1981 gggcaaacga taacctcagg cgataaagcc atgaaacgtt atttacgctg gattgtggcg
     2041 gcagaatttc tgttcgccgc agggaatctt cacgccgttg aggtagaagt cccgggattg
     2101 ttaactgacc atactgtttc atctattggc catgattttt accgagcctt tagtgataaa
     2161 tgggaaagtg actatacggg taacttaacg attaatgaaa ggcccagtgc acgatgggga
     2221 agctggatca caataacggt caatcaggat gttattttcc agactttttt attcccgttg
     2281 aaaagagact tcgagaaaac tgtcgtcttt gcactgattc aaactgaaga agcactaaat
     2341 cgtcgccaga taaatcaggc gttattaagt acgggcgatt tggcgcatga tgaattctaa
     2401 ataaaaaatt gttcggaggc tgcaatgcgt gtcaaacatg cagtagttct actcatgctt
     2461 atttcgccat taagttgggc tggaaccatg actttccagt tccgtaatcc aaactttggt
     2521 ggtaacccaa ataatggcgc ttttttatta aatagcgctc aggcccaaaa ctcttataaa
     2581 gatccgagct ataacgatga ctttggtatt gaaacaccct cagc
//
LOCUS       DAETPZ010000222         2607 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.228, whole genome shotgun sequence.
ACCESSION   DAETPZ010000222 DAETPZ010000000
VERSION     DAETPZ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.228"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..470
                     /locus_tag="K0W59_004249"
     CDS             <1..470
                     /locus_tag="K0W59_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI3024991.1"
                     /translation="TYVATREGWLYLAGVKDVYTCEIVGYAMGERMMKELTGKALFMA
                     LRSQRLPAGLIHHTDRGSQYCAYDYRVIQEQSGLKTSMSRKGNCYDNAPMESFWGTLK
                     NESLSHYRFKSRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFRIKYYQMTA"
     gene            514..1314
                     /locus_tag="K0W59_004250"
                     /pseudo
     CDS             514..1314
                     /locus_tag="K0W59_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1429..1770
                     /locus_tag="K0W59_004251"
                     /pseudo
     CDS             1429..1770
                     /locus_tag="K0W59_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1753..2590
                     /locus_tag="K0W59_004252"
                     /pseudo
     CDS             1753..2590
                     /locus_tag="K0W59_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          656 a          627 c          767 g          557 t
ORIGIN      
        1 taacgtatgt tgccacccgg gaaggatggc tgtatctcgc cggcgtcaaa gatgtttata
       61 cgtgcgaaat tgtcggttac gccatgggag agcgcatgat gaaagagttg acgggtaaag
      121 ccctgtttat ggcgctcagg agccagcgcc tacctgccgg gctaatccac cacaccgatc
      181 gaggttcaca gtactgcgca tacgattacc gggtcataca ggagcagtct ggtctgaaaa
      241 catcaatgtc gcgtaaaggt aactgttacg acaacgctcc gatggaaagc ttctggggaa
      301 cgctgaaaaa tgaaagcctg agccactatc gttttaagag ccgggatgag gccatctcag
      361 taatacggga atacattgag attttctaca atcgtcagcg tcgtcactct cgtctgggga
      421 atatctcccc ggcagccttc aggataaaat attatcagat gactgcttag aaaaagaaca
      481 aatggtggtg tccgctattg ccagtacacc tcagttctgg gccttgcccg tagcagttat
      541 ttttatcacc gggctgcact gaaagccggt gataaatacg ccacgatacg taagatgctg
      601 acagatatct ttaacagtaa ttaccggtgt tatggctatc gtcgcctgca tgcgatgctc
      661 aggcatgagg ggggtcggct atcagaaaag gttgtacgca gacttatggt ggaggaacag
      721 cttgtcgtca gtcgtaaccg tcgtcgtcgc tacagctcat attgcggaga aatcggaccg
      781 gctccggata accttatcgc cagagatttt aaggcggagc aacctaatca gaaatagctg
      841 acagatatca cggagttcca gctccctgca ggtaaagtct ggctgtcacc agtggtggac
      901 tgcttcgatg gaaaagttgt gagctggtct ctcagtacac gccctgatgc tgaactggtc
      961 aacactatgc tggatagcgc tgtcgaaacg ttaaatgctg gcgaacgacc ggtgatacac
     1021 agtgacagag gtgggcatta tcgctggcca ggctggctgg aaagagtgaa tgcagcaggt
     1081 cttattcgct caatgtcccg taaaggatgt tcacctgata atgctgcatg cgaaggcttt
     1141 ttcggcagac tgaaaacgga aatgtattat gggcgtaaat ggtcgggcat cacgccagaa
     1201 aagttcatgc agcaagtgga tgcttacatc agatggtata acgagcggcg tataaaatta
     1261 tcgctgggtg ccgtcagccc caaaatgtac cgccaacaat gcgggctgga atgataaagc
     1321 agtccaggaa atcgtccgca tccccccttc accatttgga ttgtccgctc cacccaacat
     1381 gttgtttcct taaggttcct acaccacaaa ggaaactctc atgctgcgca gtggatggct
     1441 gacgttgccg acaggcggga acttcgccag ttcaggcaga caccggaaca gcgcttcacg
     1501 caggaacagg agcatctgca gccgctgctg ggtacggact tcgatatccg tcatgtctcc
     1561 tgggatggct atatcgaggt tggcgggaac cgttacagtg tgccggaaag cctgtgtggt
     1621 cagctggtat caatacggat ctcgctggat gatgaactgc ggatctacag taatgagcaa
     1681 caggtagcat cgcaccgtct ctgttcggct gcatacggct ggcagaccgt gccggagcat
     1741 cacgctccac tctggcagca ggcttcggag cgcatggccg aacggctggg ggagatacag
     1801 aaacgggtaa tcactgtctg tgaccgggaa gcggatatct ggcattacct gtattataaa
     1861 gtcagccacg gacagcgtgg tgcgtgctgc acagaatcgc cggctggaag aggcacccgg
     1921 caagctcttc gaactgccgg aagtcctggc aaccgcagga agccacacgc tgaatgtgat
     1981 gcaaaaaggc gggcgggcag cccgtcaggc ccggatgttc atcagttaca gcgaagtcag
     2041 cataaaaaat cccgacaaca gcggccaggc gctcccgctc acgtatgtct gttgccggga
     2101 gcaggcagag gacggtgcct gctggcatct gctgacgtca ggaaaagcgg cgagtgccgc
     2161 cgatgcacga cgtattgtca gccattacga gcgacgctgg ctgacagagg aataccacaa
     2221 ggcgtggaaa agtggtggta catggaatcg ctgcgaatgc agacccggga taaccttgag
     2281 cgcatggtgg ttatccaggc gtttatagcg gtacgggtgc tgggcctgcg gcagggaggt
     2341 gtcagtgaag aaacacaaaa tgacagctgt gagaaaatac tgacgccgac agagtggaaa
     2401 ctactgtggg ttaagctgga aggaaagccg cttccggtgc aggcaccgac cctgaaatgg
     2461 gcctggggcg atggcatgac agcaaacgca caggtcgtcc cggttggggc gtcatgtggg
     2521 atggctggtc agacttcagg atatggttga gggttacttg gtgatgaagt ctcttgatca
     2581 ggagctctga tcaagagaca gcagctt
//
LOCUS       DAETPZ010000223         2602 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.229, whole genome shotgun sequence.
ACCESSION   DAETPZ010000223 DAETPZ010000000
VERSION     DAETPZ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.229"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..74)
                     /locus_tag="K0W59_004253"
     CDS             complement(<1..74)
                     /locus_tag="K0W59_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3024992.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTA"
     gene            165..1217
                     /locus_tag="K0W59_004254"
     CDS             165..1217
                     /locus_tag="K0W59_004254"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HBI3024993.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMCLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1293..2602
                     /locus_tag="K0W59_004255"
                     /pseudo
     CDS             1293..2602
                     /locus_tag="K0W59_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
BASE COUNT          629 a          589 c          666 g          718 t
ORIGIN      
        1 gcagtgcttt tgccgttacg caccacgcct tcagtagcgg agcaggaagg acatctgatg
       61 gaaatggaag ccacgcaagc accttaaaat caccatcata cactaaatca gtaagttggc
      121 agcattaccg caataagtta tgtttttaaa ttgagggcat tattatgaaa aaaataccct
      181 gcgtgatgat gcgaggtgga acctcgaggg gcgcgttcct gttagcggaa catttacccg
      241 aagatcaaac gcagcgcgat aaaatattga tggcaattat gggttccggt aacgatctgg
      301 aaattgacgg tattggcggc ggtaatccac tgaccagtaa agtcgccatt attagccgtt
      361 ccagcgatcc gcgtgctgat gtcgattatc tgtttgctca agttatcgtc catgagcaac
      421 gtgtcgatac cacgcctaac tgcggcaata tgctgtctgg cgtaggggca ttcgccattg
      481 aaaatggttt gattgcagcg acttcgccag ttacccgtgt acgtatccgc aacgtcaata
      541 cgggtacgtt catcgaagct gatgtgcaaa cgccaaatgg tgttgtcgag tacgagggta
      601 gcgccagaat tgacggcgta ccgggtactg ccgcaccggt tgcgctcact ttcctgaatg
      661 ccgctggaac gaaaaccgga aaagttttcc cgactgataa tcagattgat tattttgacg
      721 atgtcccggt gacctgtatc gatatggcga tgccagtcgt cattattccg gctgaatatc
      781 tgggaaaaac aggttatgaa ttaccggcgg aactggatgc cgacaaagca ttattagccc
      841 gcattgaatc tatccggcta caagcgggta aagcaatgtg cttaggtgat gtcagtaata
      901 tggttatccc taaacctgtg cttatttctc cagcgcagaa aggcggggca attaatgtgc
      961 gttattttat gccgcattct tgccatcgcg cgctggcgat aaccggtgct attgctattt
     1021 ccagtagttg tgcattggaa ggcaccgtca cccgacaaat cgtcccttct gtaggatatg
     1081 gcaatatcaa tattgaacac cccagtggtg cgctcgacgt tcatttaagt aatgaaggtc
     1141 aggatgccac gacgttacgc gcatctgtta ttcggacaac cagaaaaata ttttccggtg
     1201 aagtttatct tccctgaaaa aattcgttgt caggataagg acaatcaata aaggacttct
     1261 gtatgagtca tacagaaaga acaggatttt aaatgaataa gaaatcgtta tggaagctaa
     1321 ttctgatatt agcgatccca tgtattattg gttttatgcc agctccggca ggattaagcg
     1381 aactggcgtg ggtgcttttt ggtatttacc tggcggccat tgtgggtctg gttatcaagc
     1441 ctttcccgga acctgtcgta ctgttaattg ccgttgctgc ctcaatggtg gtggtcggta
     1501 acttatccga cggtgcgttt aagaccaccg ccgtattaag cggttactct tcaggtacta
     1561 cctggctggt gttctcggcg tttaccttaa gcaccgcatt tgtgaccacc ggtttaggta
     1621 aacgtattgc ctatctgctg attggtaaaa tcggtaacac catgctgggt ctgggttacg
     1681 ttacggtatt cctcgatctg gtactggctc cggcaacacc gtctaacacc gcgcgtgcgg
     1741 gcggcattgt gttaccgatc atcaacagcg tggcggtggc tttggggtcc gaaccggaaa
     1801 aaagtccgcg tcgtgtcgga cattacctga tgatgtccat ttacatggtc accaaaacca
     1861 ccagctatat gttctttacc gcaatggcgg ggaacattct ggcgctgaaa atgatcaacg
     1921 acattctgca cctgcaaatt agctggggtg gatgggcgct ggccgccgga ttgccggatt
     1981 gccggattgc cggattgccg gattgccgga ttgccgggca tcattatgct gctggtcacc
     2041 ccgctggtaa tttacaccat gtatccacca gaaattaaaa aggtggataa caaaaccatc
     2101 gctaaagcgg gccttgccga actgggaccg atgaaaatcc gcgaaaaaat gctgctcggt
     2161 gtctttgtgc tggcgctact gggctggatt ttcagtaagt ctctgggggt tgatgaatcc
     2221 actgtggcaa tcgttgttat ggcgaccatg ctgctgctgg gtatcgttac ctgggaagac
     2281 gtggttaaaa ataaaggcgg ctggaatacc ttaatctggt acggcggtat tatcggctta
     2341 agctccttat tatcgaaagt taaatttttc gaatggttag ctgaagtctt taaaaataac
     2401 ctggcatttg atggtcacgg taacgttgct ttcttcgtta ttattttcct cagcatcatc
     2461 gtgcgttatt tcttcgcttc cggtagtgcc tatatcgttg ctatgttacc tgtatttgcc
     2521 atgctggcga acgtctccgg cgcaccgtta atgttaaccg cgctggcact gttgttctcc
     2581 aactcctatg gcggcatggt ta
//
LOCUS       DAETPZ010000224         2597 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.230, whole genome shotgun sequence.
ACCESSION   DAETPZ010000224 DAETPZ010000000
VERSION     DAETPZ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.230"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            250..1287
                     /gene="curA"
                     /locus_tag="K0W59_004256"
     CDS             250..1287
                     /gene="curA"
                     /locus_tag="K0W59_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HBI3024994.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVMGVAGGAEKCRHATEVLGFDVCLDYHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1485..2150
                     /gene="mcbR"
                     /locus_tag="K0W59_004257"
     CDS             1485..2150
                     /gene="mcbR"
                     /locus_tag="K0W59_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HBI3024995.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(2433..2579)
                     /locus_tag="K0W59_004258"
     CDS             complement(2433..2579)
                     /locus_tag="K0W59_004258"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBI3024996.1"
                     /translation="MYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARM
                     RWCG"
BASE COUNT          709 a          589 c          677 g          622 t
ORIGIN      
        1 gcgttcgagc attataacga atggcatccg catagtgcgc tgggttatcg ctcgcaacgg
       61 gaatatctgc ggcagcgggc ttgtaatggg ttaagtgata acagatgtct ggaaatatag
      121 aggcaaatcc aacggattcc tcgatgaaat tattgcgtgt tgcagagggg atgtgacggc
      181 tgcaaacaaa accaatcccg tgagcctgct actattgatg gattcatgcc aaaagcacag
      241 gagagcatta tggggcaaca aaagcagcgt aatcgacgtt gggttctggc ctcgcgtcca
      301 catggcgcac cggttccgga gaatttccgt cttgaagaag atgatgtcgc cacaccgggt
      361 gaaggacagg tgttactgcg cacagtttat ttgtccctgg acccgtatat gcgtggacgt
      421 atgagcgatg agccatctta ttcaccgccg gttgatattg gcggcgtgat ggtcggcggt
      481 acggtgagcc gtgtcgtgga gtcgaatcat cctgattatc agcctggcga ctgggtgctg
      541 ggctacagtg gatggcagga ctatgacata tccagtggtg atgacctggt gaaacttggc
      601 gatcatccgc aaaatccatc gtggtcgctg ggtgtgctgg ggatgccagg ctttaccgct
      661 tatatggggc tgctggatat cggtcagcct aaagagggcg aaacgttggt ggtagctgcg
      721 gcgacaggac cagtgggggc gacggtgggg caaatcggca aacttaaagg ttgcagagtg
      781 atgggggtag ccggtggcgc ggaaaaatgc cgccatgcta ccgaggtgtt aggcttcgat
      841 gtttgccttg attaccacgc ggacgatttt gccgaacaac tggcgaaagc gtgcccaaaa
      901 ggtattgata tctattatga aaacgtgggc ggcaaggtat ttgatgcagt gctaccatta
      961 cttaatacat ctgcgcgcat tcccgtttgt ggcttagtga gcagctataa cgctacagag
     1021 ctaccacccg gtccggatcg tttacccctg ttgatggcaa cggtgctgaa aaaacgtatt
     1081 cgcttgcaag ggtttattat cgctcaggat tatggtcacc gcatccatga gtttcagaag
     1141 gagatggggc aatgggtgaa agaggataaa atccactacc gcgaagatat tactgatggt
     1201 ttagagaatg cgccacagac gtttatcggc ctgctgaagg gtaaaaactt cggcaaagtg
     1261 gtgatccgcg tcgcgggtga tgattaaagt ttcaacggcg gcgtaagccg ccataaaaat
     1321 ccaggcaaaa agcttatttt cagctttaat taactaacca cgtcattgtt ctgctgctaa
     1381 taacaacaaa ggtgggtggt ttatgattat tcactttaat acactaggtg aactccttct
     1441 gccatgcagg cagggttgga cagaaaacac gcgaaggaat taaaatgcct ggaacgggaa
     1501 aaatgaaaca tgtcagtttg actctgcagg ttgagaacga cctgaaacat cagcttagta
     1561 ttggcgcatt aaaacctggc gcacgcctga ttactaaaaa tctggcggag caattaggta
     1621 tgagtattac acctgtgcgt gaagcattat tacgtctggt ttcggtgaat gcgctttctg
     1681 tcgcacctgc acaagcattt acagtaccgg aagtgaggca aagtcagtta gacgaaatca
     1741 atcggatccg ctacgaactg gaattaatgg cagttgccct ggctgttgaa aacctctcct
     1801 cgcaagacat agcagaactt caggaattgc tcgggaaatt acagcaggcg caagaaaagg
     1861 gcgacatgga acaaatcatt aatgcgaaca ggctatttcg cttagcgatt tatcatcgct
     1921 caaatatgcc catcctgtgt gagatgattg agcaactgtg gatcaggatg ggacctgctt
     1981 tacattatct ttatgaagcg attaacccag cggaattacg ggagcgcata gaaaactatc
     2041 atctattact cgctgcgtta aaagcaaaag acaaagaggg atgcaaacat tgtcttgctg
     2101 aaattatgca acaaaatatt gctattttat accagcaata caatcgttaa attaaatgcc
     2161 cggcaaatac cgggcatcaa gtaattactc aaatcgccac gcaatattca tgccgacacc
     2221 ataatttcgt ccgggcgcag gttcatcgat aggaattaaa accccaaaaa gattaaaaaa
     2281 acaccacaaa acggatgttt cttcaacacc acttttgctc catatgaacg gaaccgacga
     2341 ttaaactgga tggctctgat tgattcaggg tatgaatggc ggttttttgc tccgtttccc
     2401 tcaaaatgga cgcaacttcc cctctgcggc tctcagccgc accaccgcat ccgggccagc
     2461 ggctcatgca tcaggacctg ctctgccaga cggtagcccc gcttcagccc cgtaaaacgc
     2521 atctgactcc cgcacagcac gcacttcagc gggtcaacct tcagtaacct ctgatacatc
     2581 gtaagcgccc catcagc
//
LOCUS       DAETPZ010000225         2567 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.231, whole genome shotgun sequence.
ACCESSION   DAETPZ010000225 DAETPZ010000000
VERSION     DAETPZ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.231"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..489
                     /gene="capU"
                     /locus_tag="K0W59_004259"
     CDS             <1..489
                     /gene="capU"
                     /locus_tag="K0W59_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HBI3024997.1"
                     /translation="EFMLNLLFHLKMNGRQFCWLIVGSGSPELREHLQYQIDSMGMHD
                     DVFIADNVFPAAPVYRVASLVVLPSENESFGMVLAEASAFSVPVVATQIGGIPEVIQN
                     NQTGTLLPASNKHAWMCALNDFFNDPGRFYQMARLAKQDIEERFDINKTALKILTLAK
                     QK"
     gene            494..1444
                     /gene="virK"
                     /locus_tag="K0W59_004260"
     CDS             494..1444
                     /gene="virK"
                     /locus_tag="K0W59_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HBI3024998.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            1509..2453
                     /gene="lpxM"
                     /locus_tag="K0W59_004261"
                     /gene_synonym="msbB"
     CDS             1509..2453
                     /gene="lpxM"
                     /locus_tag="K0W59_004261"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBI3024999.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          741 a          489 c          582 g          755 t
ORIGIN      
        1 gaatttatgt tgaatttact gttccattta aaaatgaatg gacgacagtt ctgttggttg
       61 attgtggggt ctggttcacc tgaactgcgg gaacatttac agtatcagat tgacagtatg
      121 ggcatgcatg atgatgtttt tattgctgac aatgtttttc ctgccgcccc cgtatatcgg
      181 gttgccagtc tggtggttct gccttcagaa aacgaatctt ttggtatggt gctggcagaa
      241 gcatcggcat tttctgtgcc tgtagtggcc actcagattg gtggaatccc tgaggttatt
      301 cagaacaacc agaccgggac attgttacca gcaagtaata agcacgcatg gatgtgcgcc
      361 ctgaatgatt tttttaatga ccctgggcgt ttttatcaga tggctcgcct ggcaaaacag
      421 gatatagaag agcggtttga tattaataaa actgcgttaa aaatactcac attagcgaag
      481 caaaagtaac aatatgtttt ctataagtaa cttatcattt atcggtttcc ttaaaaggat
      541 tattttttcc tcagattcac taccggggaa gtgggaacac agaaaatttc ggttcatgta
      601 cattttgcga tgttctataa atccggttgt cagtattcga tattattatg aactgcgttc
      661 cttgccgtgc attgaggata ttctggcaat acaccccacg ttgccagcaa gaattcatcg
      721 cccttatttg cataaagggg ggcgcgcctg gacccgtgga caatatattc ttgagcatta
      781 tcgctttgtg caaaatttgc cagaaaaata ttctaaattt ttgtttccac aaaaatcggt
      841 atcattagtc caattcatcg ggaaagatgg agagaatttt gatatccagt gttctcccag
      901 tggttttgac cgggaaggag agttgatgtt gtcattgttt tacaataaaa cagttattgc
      961 ccggttaaca ttttcagtca ttctgactca aaatggacac attgctttta ttggcggatt
     1021 acagggagcc cctaaaaata ccggacctga tgttattcgg tgcgcaaccc gggcctgtta
     1081 tgggattttc ccaaaacgga ttatttttga agcattttgt gcgttgatga aagcttgcaa
     1141 tatctctgag tgtctggcag tcagtgagca cagtcatgtt ttccggcaat tgagatactg
     1201 gtatcagaaa cgcaaaacct ttgttgcggt ctatagtgat ttctgggagt ctgtagcagg
     1261 aaaaacctgt ggtgactggt acagattacc aacccaggtg attcgaaagc cgctaagcga
     1321 tatagccagt aaaaaacgct ctgagtaccg aaaacgatac gctttgctgg attatatcca
     1381 tgaaactact atccgctctt tggatgcata tcctgtacac tcagaacatc agaatttaaa
     1441 ttaaggtttg atgtattctc tgaataaaac agtaatgata attttggtag aggcattcgc
     1501 actaaataat gaaaaaatat aaatctgagt ttattcctga atttaagaaa aattaccttt
     1561 cccctgttta ctggtctaca tggttccttt tgggaatgat tgcaggtatt tcaatgtttc
     1621 ccccttcatt cagagatcct gtcttggcaa aaatagggcg ttgggcgggc agattgagca
     1681 aaaaagctcg tcgcagggcg acgattaatt tatcgctttg tttcccagaa aagagtgata
     1741 cagaacggga aataattgtc gacaaaatgt ttgtcacagc attgcaatcc atagtgatga
     1801 tggcagaact ggcaattcgt ggtccggaaa agttccagaa acgagtgttc tggaaaggcc
     1861 tcgaaattct tgaggaaatt cgacacaata atagaaatgt gatttttctg gttccccatg
     1921 gctggagcgt ggatattcct gcaatgttgc tggcagctca gggaaaaaaa atggccgcca
     1981 tgtttcatca gcaacgaaat ccagtgattg attatgtctg gaattcagta cggcgtaaat
     2041 tcggggggcg cttacatgcc cgggaggatg ggataaaacc atttattcag tcagtacgcc
     2101 agggatactg gggatattac ctcccagatc aggatcatgg tcctgaatac agtgaatttg
     2161 ctgatttttt tgcgacctat aaagcgacat taccaatcat tggacgtctg atgaacatca
     2221 gtcaggctat gattatacca cttttcccgg tttatgatga aaaaaaacat ctactgacta
     2281 ttgaaattcg gccaccaatg gatgcttgca ttgccagcgc ggacaataaa acgattgccc
     2341 gacaaatgaa caaaacagtg gaaattttgg tggggccaca tccggaacag tatgtctggg
     2401 ttttaaaatt gttaaaaaca cgcaaatcaa acgaagcgga cccgtaccct tgaaattata
     2461 aagtacaagt atttccacta gttgtttgta agcgtacagc ctgaaccgtc tggtcagaat
     2521 ctgacgaatt agacaaagtg gtgtccacca aataagtagt gggaacc
//
LOCUS       DAETPZ010000226         2540 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.232, whole genome shotgun sequence.
ACCESSION   DAETPZ010000226 DAETPZ010000000
VERSION     DAETPZ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.232"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..1568
                     /locus_tag="K0W59_004262"
     CDS             <1..1568
                     /locus_tag="K0W59_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HBI3025000.1"
                     /translation="FPNLLKYDPDQFGPDLIEQLAQSGKYSQDNTKGDAMIGVKQPLP
                     KAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQNAIDAVASLPSGGELFIPASNQA
                     VGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLTGSVLRLSYDSDTIGRYLRNIRVTGN
                     NTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEVETAYLMQGLWHSKFIACQAGTCRVG
                     LHFLGQCVSVSVSSCHFSRGNYSADESFGIRIQPQTYAWSSEAVRSEAIILDSETMCI
                     GFKNAVYVHDCLDLHMEQPDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGI
                     YFRTPTSTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTGDEWAVNI
                     VDINECVSFDKCIFNKPLCYLRSGGVSVTDCYLAGITEVQKPEGRYNTYRGCSGVPSV
                     NGIINVPVAVGATSGSAAIPNPGNLTYRVRSLFGDPASSGDKVSVSVVTINVTRPSPV
                     GVALPSMVEYLAI"
     gene            complement(1602..>2540)
                     /locus_tag="K0W59_004263"
     CDS             complement(1602..>2540)
                     /locus_tag="K0W59_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBI3025001.1"
                     /translation="HHYALSGQPEPYLFGFESAGGIAVIIFFSISGYLISKSAIRSDS
                     FIDFMAKRARRIFPALVPCSILTYFLFGWILNDFSAEYFSHDIVRKTISSIFMSQAPD
                     ADITSHLIHAGINGSLWTLPLEFLCYIITGVAVALLKNGKAFIVILLVFVSLSLIGSV
                     SENRDVMFSIPLWLYPLRGLAFFFGATMAMYEKSWNVSNVKITVVSLLAMYAYASYGK
                     GIDYTMTCYILVSFSTIAICTSVGDPLVKGRFDYSYGVYIYAFPVQQVVINTLHMGFY
                     PSMLLSAVTVLFLSHLSWNLVEKRFLTRSSPKLSLD"
BASE COUNT          763 a          515 c          579 g          683 t
ORIGIN      
        1 attttccgaa tttgctgaag tatgaccctg atcagttcgg tccggattta attgagcaac
       61 tagctcaatc cggtaagtat tcgcaggata acaccaaagg cgatgccatg attggcgtca
      121 agcagccttt accaaaagca gttttaagaa ctcagcatga caaaaataaa gaagcaataa
      181 gtatcctgga ttttggtgtt attgatgatg gtgtgacaga taattaccag gcaatacaaa
      241 atgcaataga tgccgttgct tcactaccct ccggcgggga gctgtttatc cctgcgagca
      301 accaagcggt ggggtatatt gttggatcca ctttgcttat tcctggcggt gttaacatca
      361 gaggggttgg taaggcatcg caactccgag caaaaagcgg acttacagga tctgtgttaa
      421 ggctgtctta tgattcagac actatcggcc gttatctgag aaatatacga gtaactggta
      481 ataacacctg caatggtatt gacacaaaca ttacagcaga agactctgtc atcagacagg
      541 tttatggctg ggtatttgat aatgtaatgg tgaatgaagt tgaaaccgct tatttaatgc
      601 aagggctctg gcactcaaaa tttatagcat gtcaggctgg aacctgtaga gtcggtcttc
      661 actttttagg ccagtgcgta agtgttagtg tcagctcctg ccatttcagc agaggaaatt
      721 attctgctga tgaaagcttt ggcatcagga ttcagcctca aacgtatgcg tggtcgtcag
      781 aggcagttag gtcagaagca ataattttag acagtgagac catgtgcatt ggttttaaaa
      841 atgccgtcta tgttcatgat tgccttgatt tgcatatgga acaaccggat ttagattatt
      901 gcggttcaac aggcgtggtt atagagaatg taaacggagg attttctttc tcaaactcat
      961 ggatagcagc agatgccgat ggcactgaac aatttacggg gatatatttt agaacgccga
     1021 cctcaacgca gtcacataaa attgtcagtg gtgttcatat caacactgca aacaaaaaca
     1081 cggctgcaaa caatcaaagt atagcgatag aacagtcggc gatcttcgtc tttgtaagtg
     1141 gttgtacgtt aactggtgat gaatgggctg taaatattgt cgacatcaat gaatgtgttt
     1201 ctttcgataa gtgcatattc aataagcctc tatgctatct tcgtagcggt ggcgtgtcag
     1261 ttactgactg ttatttggct ggcattaccg aggtgcagaa accggaaggc agatacaata
     1321 cgtatcgtgg ctgttcaggc gtaccgtctg taaatgggat cattaatgtc ccagttgcgg
     1381 taggtgccac aagcggatct gcagctatac cgaacccggg gaacctgaca tacagagtaa
     1441 gaagcctttt tggtgaccct gcatcaagtg gtgacaaggt tagtgtttcc gtggtgacaa
     1501 ttaatgttac tcgcccaagc ccagtaggag tcgcgctgcc ttcaatggtt gagtatctgg
     1561 ccatctgatt attttagggg ccatatggcc ccttttattg ttcaatccag ggataattta
     1621 ggcgaacttc gtgtcaggaa tcttttttca accagattcc atgaaagatg ggacaagaaa
     1681 agaacagtca cggcacttaa caacattgac gggtaaaaac ccatgtgcaa tgtgtttata
     1741 acaacctgct gaaccgggaa tgcataaatg tacacaccat acgagtaatc aaacctgccc
     1801 ttaaccagtg ggtcgccaac ggatgtgcat atggctattg tcgagaatga aaccaggata
     1861 tagcaagtca tagtataatc aatgcctttg ccataactag cgtaggcgta catagcaagc
     1921 aaagaaacta cagtaatttt aacattactt acattccatg acttctcata cattgccata
     1981 gtggcaccaa aaaagaacgc cagtccacgc aaaggataca gccataacgg aatagagaac
     2041 attacatccc tgttttctga cacacttcca attaatgaaa gagaaacaaa aaccaacagt
     2101 ataactatga atgcttttcc gttctttagt agggcaactg ctactcccgt tattatataa
     2161 cacaggaact caagcggtag ggtccacaaa ctgccgttaa ttccggcatg aattaaatgt
     2221 gatgtaatgt cagcatctgg agcctgactc ataaaaatag agcttatggt cttcctgaca
     2281 atgtcatggc taaaatattc tgcgctgaag tcgttaagta tccatccaaa taaaaaatat
     2341 gtcagtatag agcaagggac cagcgccggg aatattcttc tcgctctttt agccatgaaa
     2401 tcaataaagg aatcactcct gatagctgat tttgaaatca gatatcctga tattgaaaag
     2461 aatatgataa ctgcaatgcc gccagcactt tcaaagccga acaggtacgg ctccggctgc
     2521 ccagagagcg cgtagtggtg
//
LOCUS       DAETPZ010000227         2540 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.233, whole genome shotgun sequence.
ACCESSION   DAETPZ010000227 DAETPZ010000000
VERSION     DAETPZ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.233"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..816)
                     /gene="ipaH7.8"
                     /locus_tag="K0W59_004264"
     CDS             complement(<1..816)
                     /gene="ipaH7.8"
                     /locus_tag="K0W59_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HBI3025002.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGPQIYFSMSDGQQNTLHR"
     gene            1145..1324
                     /locus_tag="K0W59_004265"
                     /pseudo
     CDS             1145..1324
                     /locus_tag="K0W59_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1341..2031)
                     /locus_tag="K0W59_004266"
                     /pseudo
     CDS             complement(1341..2031)
                     /locus_tag="K0W59_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1954..2205
                     /locus_tag="K0W59_004267"
     CDS             1954..2205
                     /locus_tag="K0W59_004267"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3025003.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAII"
     gene            complement(2225..>2540)
                     /locus_tag="K0W59_004268"
     CDS             complement(2225..>2540)
                     /locus_tag="K0W59_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3025004.1"
                     /translation="ISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRG
                     IYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLA
                     KV"
BASE COUNT          713 a          489 c          589 g          749 t
ORIGIN      
        1 gcgatggagt gtattctgtt gtccgtcact catggagaag taaatctgtg ggccgtggta
       61 gtccggcgaa gaggttaatc tttgcaggga ttgcagaacg cgagtcgaca atggattccc
      121 tgaaatatta atcctgtaac ttgaatctaa ttgtgaaaaa ctctccggaa gagtggttat
      181 ctgattaaaa tgaaaataat attccttttg tctgatattg tttctatcag aaaattgagg
      241 aaaagtaacc agattattgc gctgagcaca gagaatttcc agtttatccg gtaagtcggg
      301 taatgttgtc agcctgttat aataaacatg taaaaatttc aatttttcgg gaagagccgg
      361 taagttatat agcctgttat tttcaacaaa taatgatgtt aaatggtttg gcaaaacagg
      421 aaggttttcc agttgattag aacgaacact taaagttgtt aatgactcag gaagttctgg
      481 tatcacagaa agcctgttat tatcagcatt aagttctttg agaaatgcag gcagctttgg
      541 caatgatgtt aatttattat tttctacaat caaagcagaa atatgctttg gtaattcggg
      601 aagagacgtc aaatttaggt ctgataaatt taatactgct tcttgattct gaaagcactg
      661 ggagagtctg ttaaaagcaa tgcctcgctc ttccccggga gaagagttct tttcccattc
      721 agtcaggatt ctcagataat gttcattact ggttgagttt gagttaatag agggggagca
      781 agaaactgat gagtgtgtat tatttacaga gaacataatg ctaggtcaac cttatatttg
      841 tgagaataaa tatttaaaat tgtatattta tacaaatttc acttaccatt tccgatatta
      901 agttttaata gtttccattt tacacataac agaagttatg ttgtaattta tttatggagg
      961 ttttctatgg aattcttttt tattgtgact gcagtcaata ataacactga attcaacatt
     1021 ccagtgagca aggaaatgta atccgagtaa gtatatttgt tttacatcat attttagtca
     1081 agctcattta ttcctattat agaatgataa attaaaatta ctgtaaaaaa acgatacaca
     1141 tttaatacga gggaggccgg cagaaaaacg gcttgcggta aggcaggaga aatgtgtgcc
     1201 gcgaatgaag tccttgtatg actggataaa gctccagagg aaaactttgt caaaccatgc
     1261 tgaaatggta aaggcgttca actatatcct gaatcactgg aatgcactga atgagttttg
     1321 ccgggtagta cttccaatca ttagaatatg tgcttttcaa tgaaggaacc gatgactttt
     1381 tcgtggattt ccacggaacg cgagaagcag attgttttac gtgccagccg cttgatgcgg
     1441 gggcggaggg tcaggttgtt acgttcatac gttgcgtgaa gattttgccg gtcaggtgtt
     1501 tctcctccgg cacttctctg gcgtagctgc cccagtcatc gctggtcacc atgccgatac
     1561 agaacggagt caacagggct aacagttccc gacaggtctc atcattccgt ggcccgcaag
     1621 tgtaagccaa cacaccgcca gttttagtgt tgtacgcata ccagggccag tgttgttgcg
     1681 ccttgctgcc aacgaagcgc cactgctcat caagttcaca gataagggca acatcagcgt
     1741 gagcgactgg cgcagatgtt atccgccggg gcgagagctt tttaatgtcc gtatgacggt
     1801 attgatgccg attttcagtg ttctggcggt ataacagccc cctgcgccgt tatgtgccat
     1861 ttcaacgatc tgctccttaa cgccgagttt tctggcttca taagagtaag tgagctggaa
     1921 aacacgttta catgagcgac aacggaaacg ctcatgccgg gaacaactac ggccatgtcg
     1981 ataaacttca tcggaattac agcgcggaca atgaacagta acggtagtca tggaaaacct
     2041 caaataccag tagattacca gaaacttcta tcgattgaag acattacctt ttgccgcaat
     2101 aggtggggta acattggtga aaacgcatta cgccgggtgg cagttggaaa aaaattatct
     2161 tttttcggct ctgatcgtgg tggagaaaat gcggcaataa tctaaatagc tgcgcttaat
     2221 accgctacac ttttgccagc ccatgtttgc ctccggggaa tgggctgacg gtttccataa
     2281 aatggcgaac ttttttcaac agttgccaca ttgagcggca ctgatgatta cgcgttattg
     2341 tgtcgtgaag tgcctgccat agccgttcaa catgattcac ccatggcgag taaaccggct
     2401 gataaatgcc cctgaacttc gggttctcct tcagccagct ctgtgtttcc cggcttttgt
     2461 ggataatgta gttgtccacg atcagcgtga tggttttcgc ccgacggtat gtcgctttaa
     2521 gccgcttcag caggctgatg
//
LOCUS       DAETPZ010000228         2503 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.234, whole genome shotgun sequence.
ACCESSION   DAETPZ010000228 DAETPZ010000000
VERSION     DAETPZ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.234"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            155..1108
                     /locus_tag="K0W59_004269"
                     /pseudo
     CDS             155..1108
                     /locus_tag="K0W59_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(1149..2144)
                     /gene="pgl"
                     /locus_tag="K0W59_004270"
     CDS             complement(1149..2144)
                     /gene="pgl"
                     /locus_tag="K0W59_004270"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HBI3025005.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYSVGQGPMWVVVNAH"
BASE COUNT          692 a          613 c          561 g          637 t
ORIGIN      
        1 tattgcgata tggttacatt aaggacaaag catctctaat gactttagct caacgattaa
       61 taacattttt tcattaattt aagttggcta aatgcagtta aaatttttaa cgaccagccc
      121 cccaaaatcg tcgcttccat tttccggagt aataatgaag catgaattat caagtatgaa
      181 ggcatttgtc atactggcag agtccagttc atttaacaat gccgctaaat tactcaatat
      241 tacgcaacct gcattaacgc gaagaataaa aaagatggag gaagatttac atattcaact
      301 atttgagcgt acaactcgca aggttacgtt aacaaaagca ggaaaaaggt tgctcccgga
      361 ggcgcgggaa ttaataaaga aattcgatga aacgcttttt aatattcgtg atatgaatgc
      421 ttatcatcgt ggtatggtga cattagcgtg tattccgacc gcagtatttt attttttacc
      481 gctggcaatc ggtaaattta atgagctgta tcccaatatt aaagtgcgga ttctggaaca
      541 aggcacgaat aattgcatgg aatcggtgct gtgtaacgaa tcagactttg gcataaacat
      601 gaacaacgtc acaaattcat ccattgattt taccccactg gtcaacgagc cattcgtgct
      661 ggcctgtcgg cgtgaccatc cattggccaa aaagtagctt gtagaatggc aggagttagt
      721 gggttacaaa atgattggcg tacgttcctc cagcggcaac cgattgttga tagaacaaca
      781 gctagccgac aagccctgga agctggactg gttttacgaa gtgcgtcatc tttcgacgtc
      841 attaggacta gtggaggcag gactggggat ttcggcgctt cctggtctgg caatgcccca
      901 tgcgccctat tcttcgatta ttggtatccc actggtggaa cccgttatac gtcggacatt
      961 ggggattatt cgccgcaaag atgccgtgct ttctccggca gctgaacgct tttttgcctt
     1021 gctgattaat ttgtggactg acgataaaga caatttgtgg accaatatcg tcgaacgcca
     1081 gagacatgcg ttacaggaga taagttaata caccggcgca ggagagactg cgccggttaa
     1141 atcagcggtt agtgtgcgtt aaccaccacc cacattggtc cctgcccgac cgaatagcgg
     1201 cctttttcat gcagtagccc ctgctcgcca acaatttcgt ataccgagat gtggtgagat
     1261 ttttgcccag cggcaatcag atacttgccg ctgtaatcaa cattgaagcc gcgcggctgg
     1321 gtttccgttg gctggaagcc ttctttactc aacacgctgc catcttccga aacgctgaaa
     1381 acggtaatca gactggcggt acggtcgcag gcgtataaat ggcgaccatc cggggtgata
     1441 tgaatatcag ccgcccaacg ggtgtcggag aagttttccg gcatcatatc cagcgtctgg
     1501 acacattcga tattgccgtg cggatctttc agttcccaga catccactga gctgtttaac
     1561 tcattgacgc aataagcata ttgttcgttt ggatggaata ccatatgacg cgggccggcc
     1621 ccttcaacgg tagtcacttc cgcagggtcc tgcgccacca gatgaccatc atcgctgacc
     1681 gtaaacaggc aaatgcgatc ttgctttaat gccggaaccc acagcgtacg gttgtccggt
     1741 gagatattgg cggaatggca accgtccagc ccctcgacta catcgacgac gcccactggc
     1801 aggccatctt ccagacgcgt tacgctcacg ttacccgcat tgtaagaacc tacaaagaca
     1861 aactgcccct ggtgatcggt ggaaatatgc gtcggactac ccggcagcac agactctgcg
     1921 gcaaaggtca gtgcgccatc gtccggggca atacgatacg ccaggacgcg aaactcaggg
     1981 cgaacaccaa catagagata acgtttgtcc gggctgacca ccatcggctg cacctgcccc
     2041 ggcacatcga caacctgtgt cagcgtcagt gcgccttcat gattcaggtt ccagacgtga
     2101 atttgctggc tctcagggct ggcgatataa actgtttgct tcatgaacac acctcaaaaa
     2161 tgataaatcc cttatctttc atacatataa caacacttat tggataaaaa acagacaaca
     2221 aaacacgata aaatacaata tcgcacatca attaaatcaa taagttagca ctccatttcg
     2281 aatggcatat tactaaaata ttagtcaccg acacgaattt gacaatgccg cagatatttt
     2341 cgcgactatt cagggagtat gactctcccc gcttttgttt acctgtatca acgaccgtaa
     2401 acactatgcc ataacaactt ctggccaact tcccattcgc tcaacaatca actcacttcc
     2461 ggcacaaata aacgcattac gttgtagatc ggtattctgg tca
//
LOCUS       DAETPZ010000229         2486 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.235, whole genome shotgun sequence.
ACCESSION   DAETPZ010000229 DAETPZ010000000
VERSION     DAETPZ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.235"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            154..267
                     /locus_tag="K0W59_004271"
                     /pseudo
     CDS             154..267
                     /locus_tag="K0W59_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
     gene            complement(400..912)
                     /locus_tag="K0W59_004272"
     CDS             complement(400..912)
                     /locus_tag="K0W59_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HBI3025006.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1107..1871
                     /locus_tag="K0W59_004273"
     CDS             1107..1871
                     /locus_tag="K0W59_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3025007.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQTLKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIGGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGECDLNDFYSDIQTDAESGFT"
     gene            1822..>2486
                     /locus_tag="K0W59_004274"
     CDS             1822..>2486
                     /locus_tag="K0W59_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBI3025008.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          701 a          551 c          633 g          601 t
ORIGIN      
        1 gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc tgggaattca
       61 tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca attttcacaa
      121 gacgtcgctg atggggcgct tactcctcag ccgaaacctc cagcgcctcc ggcatgggca
      181 atgatgccac cgtcgaactc tctccagttg ctggacgaaa cgttctcggt atccgggacg
      241 gaatcatcag cgaccagaca gcattgagaa tgcttcagga gtacatcagg atccaatgcc
      301 ttggggggta gcggtaattt tactcattat ccttcaaatc agattctgtt gtcagaggaa
      361 tgggggaagc tggattcgca agagcttttt atgggcagtt tagctaaaaa aaggcaacac
      421 gaagctaaaa ctaccaacat aaagtgctga ccagctagca gctgaaatca gactcactgt
      481 atacactttc actggatgaa gttgcaacat tcctgccacc aaaggagcaa tgtaacgcag
      541 cacggcgata aagcgcgaag tgaaaagtat tgacacagag ttattttgca actgaaggcg
      601 aacccgttct attgtattgg aacggctcga tattatacat gcaagttgag ggatatgccc
      661 aattattata ccaagatggt aattaacgat agtaccgcac catgcgccac tcatgaccgt
      721 gattccagct tcccatggtg aaagagtcgt cttgctgact gtaattacag tggccatcat
      781 aattgatgct ggtggcaata tcgccgaaat gagtaaagtg gattttgtga acgctattat
      841 aaaaagaagc ccccataggc gatatggatg aagtacaaaa tagttcatca aagcatttat
      901 ccactccatc aatgtctttc cttctgtgaa tttttctttg ctatttaacc gtgatacgac
      961 ttaatttagg cttaaacacc ttacatttac aaggtactcc tgtggggggc cctgccacgg
     1021 agcgtcgcag gagtgagtga tgaggaagaa ttaaagcaac ctattgtgac aggcaacgat
     1081 aacagtaaat cacaggaagc ataatcatgg caaaaccgga ctgggaggcc atcgaatcgg
     1141 cataccgggc cggagtcctt agcctccgtg atataggcga gaaatacggc gttacagaag
     1201 gggctatcag gaagagggcc aaaaagcttg gttgggcacg cagtggcggt acgcaggttt
     1261 gcaaaaatgg tacgcaaaaa aggaaagtgc gtaccagcag aaagcctgcc attactggcc
     1321 ttacacaaaa aagtacgcaa ctaaaaacag aatctacacc ggatacgaaa ccgatacgcg
     1381 gaatgcgtac cgatcccccg actaacccat tccaacccgg taaccagcag acattaaaac
     1441 acggtggtta tgcccgtcgc cttctgctca aagatgaggt gatagaggac gctaaagcgt
     1501 tgacgctcga ggacgaatta tttcgccttc gtgctaacaa ccttgtcgct gcagagaata
     1561 ttggtcggtg gttggtgtcg ctggaagatg ctaatgggga ccaggaaagg aagatgctga
     1621 ttgaaaatat cagcgccgcc gagaaagcaa tgatgcgcaa tacagttcgt attgagtcca
     1681 tcggtggcac gcttgcgacg gtaggaaaaa tatttgctga tacagcctac cgcaaggccg
     1741 ccactgataa ggtgtctctg gaggctgatc gtcttcgccg tgatgcaggt attgatgatg
     1801 gcaatggaga gtgtgacctc aatgacttct actctgacat ccaaaccgac gctgaatccg
     1861 gctttacgta gtttctggac tatgcgggca cgtaacaaag tgctttatgg tggtcggtca
     1921 tcgtcaaaat catgggatgc cgctggcatt gccatatttc tgtcgaataa atacaccctg
     1981 cgtttttgtt gtgcccgtca gatccagaat aaaatcgaag agtcggtgta taccctgctc
     2041 aaaattcaga tagacaggtt tggtctgcgg caccgtttcc gtattctgaa caacaaaatc
     2101 attaaccggg ttactggctc ggaatttgtt ttttatggat tatggcgcaa catcgaagaa
     2161 attaagtcac tggaggggat cgatgtgttg tggctggaag aagcccacgc actgacggaa
     2221 taccagtgga aaattctgga gccaacgatc cgtaaagagg gttcggaatg ctggttcata
     2281 ttcaaccccg gacttgttac tgatttcgtc tggcgcaact tcgttgttga tccgcccgaa
     2341 ggcactctca tccgcaaaat taactatgac gaaaatccgt ttctgtctga caccatgctt
     2401 aaggttatcg acgcggcgcg acgccgtgat ccggatggtt ttaaacatgt gtatgagggc
     2461 gttccggagt ctgatgatga tgcggc
//
LOCUS       DAETPZ010000230         2457 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.236, whole genome shotgun sequence.
ACCESSION   DAETPZ010000230 DAETPZ010000000
VERSION     DAETPZ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.236"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            9..2243
                     /gene="xdhA"
                     /locus_tag="K0W59_004275"
                     /pseudo
     CDS             9..2243
                     /gene="xdhA"
                     /locus_tag="K0W59_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004089117.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            2254..>2457
                     /locus_tag="K0W59_004276"
     CDS             2254..>2457
                     /locus_tag="K0W59_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBI3025009.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGS"
BASE COUNT          593 a          649 c          648 g          567 t
ORIGIN      
        1 aatcatgcat gcgcgtcgat tccattgcta aggtcaccgg gcgggcacga tatactgacg
       61 attatgttat ggcgggcatg tgttacgcga aatatgtacg tagccctatc gcacatggtt
      121 atgccgtaag tattaatgat gaacaagcca gaagtttacc gggcgtactg gcgattttta
      181 cttgggaaga tgtgcctgat attccattcg ctacagctgg gcatgcctgg acacttgacg
      241 aaaacaagcg cgataccgcc gatcgcgcac tgctaactcg ccatgttcgt catcatggtg
      301 acgccgttgc catcgtcgtg gcccgcgatg aactcacggc agaaaaagcg gcgcaattgg
      361 tcagcattga gtggcaagaa ttacccgtta tcaccacgcc agaagcggcg ctgacagaag
      421 acgctgcacc aatccataac ggcggcaatt tactgaaaca aagcacgatg tcgacgggca
      481 atgtccaaca aacaatcgat gccgccgact accaggtaca ggggcactat cagactcccg
      541 ttattcaaca ttgtcacatg gaaagcgtaa catcgctggc gtggatggag gatgactcgc
      601 gaattaccat cgtttccagc acccagatcc cgcacattgt tcgccgcgtg gttggtcagg
      661 cgctggatat tccctggtca tgcgtacgag tcatcaaacc gtttgtcggt ggcggttttg
      721 gtaataaaca ggatgtactg gaagagccaa tggcggcatt ccggtgaaag tttcccttag
      781 ccgtgaagag tgtttcctcg caacccgtac ccgccacgct tttactattg acgggcaaat
      841 gggcgtgaac cgcgacggaa ccttgaaagg ttatagtctg gatgttctgt ctaacaccgg
      901 cgcttatgca tctcacgggc actccatcgc ttctacgggg ggggaataaa gtcgcttacc
      961 tttatcctcg ttgtgcctac gcttacagtt caaagacctg ctataccaac ctcccctcgg
     1021 ctggtgcgat gcgtggttat ggcgcgccac aagtcgtatt tgcctttgag tctatgcttg
     1081 atgacgccgc gacagcgtta ggtattgatc ctgttgaaat tcgtttacgc aacgccgccc
     1141 gcgaaggaga tgctaatccg ctcacgggca aacgtatttg cagcgcaggg ttgccggagt
     1201 gtcttgaaaa aggccggaaa atctttgaat gggaaaaacg ccgtgcagaa tgccagaacc
     1261 agcaaggcaa tttgcgccgc ggcgttggcg tcgcctgttt tagctacacc tctaacacct
     1321 ggcctgtcgg cgtagaaata gcaggcgcgc gccttctgat gaatcaggat ggaaccatca
     1381 acgtgcaaag cggcgcgacg gaaatcggtc agggtgccga caccgtcttc tcgcaaatgg
     1441 tggcagaaac cgtgggggtt ccggtcagcg acgttcgcgt tatttcaaca caagataccg
     1501 acgttacgcc gttcgatccc ggcgcatttg cctcacgcca gagctatgtt gccgcgcctg
     1561 cgctgcgcag tgcggcacta ttattaaaag agaaaatcat cgctcacgcc gcagtcatgc
     1621 tacatcagtc agcgatgaat ttgaccctga taaaaggcca tatcgtgctg gttgaacgac
     1681 cggaagagcc gttaatgtcg ttaaaagatt tggcgatgga cgctttctac caccctgaac
     1741 gcggcgggca gctctctgct gaaagctcca tcaaaaccac cactaaccca ccggcgtttg
     1801 gctgtacctt tgttgatctg acggtcgata ttgcgctgtg caaagtcacc atcaaccgca
     1861 tcctcaacgt tcatgattca gggcatattc ttaatccact gctggcagaa ggtcaggtac
     1921 acggcggaat gggaatgggc attggctggg cgctatttga agagatgatc atcgatgcta
     1981 aaagcggcgt ggtccgtaac cccaatctgc tggattacaa aatgccgacc atgccggatc
     2041 tgccacaact ggaaagcgcg ttcgtcgaaa tcaatgagcc gcaatccgca tacggacata
     2101 agtcactggg tgagccaccc ataattcctg ttgccgctgc tattcgtaac gcggtgaaga
     2161 tggctaccgg tgttgcaatc aatacactgc cgctgacgcc aaaacggtta tatgaagagt
     2221 tccatctggc aggattgatt tgaggataac atcatgtttg attttgcttc ttaccatcgc
     2281 gcagcaaccc ttgccgatgc catcaacctg ctggctgaca acccgcaggc caaactgctc
     2341 gccggtggca ctgacgtact gattcagctc caccatcaca atgaccgtta tcgccatatt
     2401 gttgatattc ataatctggc ggagctgcgg ggaattacgc tggcggaaga tggctcg
//
LOCUS       DAETPZ010000231         2436 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.237, whole genome shotgun sequence.
ACCESSION   DAETPZ010000231 DAETPZ010000000
VERSION     DAETPZ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.237"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..1334
                     /locus_tag="K0W59_004277"
     CDS             <1..1334
                     /locus_tag="K0W59_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005029490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HBI3025010.1"
                     /translation="VFSITKKHQPYEFKNFISAWLDTEHIDRGLLTKEWRKGNRVSRI
                     TRTTSGANAGGGNLTDRGEGFVHDLTSLTRDVATGVLARSMDVDIYNLHPAHAKRIEE
                     IIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIGIEVIPAHVTEYLNKVLTE
                     TDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQPSGAMTDEQATAETVEPNA
                     TEHHQNTQPLDAQSQVNSVDEKYQELRAELHEARKNIPPKNPVDADKLLAASRGEFVE
                     GISDPNDSKWIPGHHISSNEVKKTENEVRQTEDKQHQNSEPEEGTPCNQTGEDNCPDC
                     GAVMGDATYQETFDEKKPDEAQEEEPKKTEKADDLLPENAGSDQHNDSNNETGEDELN
                     WKKQVLIAAVYGLCANPACIAYAPAIPDIAMMIANKLENFGVTSDERLPDF"
     gene            1312..1608
                     /locus_tag="K0W59_004278"
     CDS             1312..1608
                     /locus_tag="K0W59_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HBI3025011.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLF
                     ESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            1614..>2436
                     /locus_tag="K0W59_004279"
     CDS             1614..>2436
                     /locus_tag="K0W59_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI3025012.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSILIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIEFLNLGGEHLGIDTSRDNSELFANDGTPTWLKEK"
BASE COUNT          740 a          574 c          602 g          520 t
ORIGIN      
        1 cagttttctc tatcacgaaa aaacatcagc cctatgaatt taagaatttc atttcagcct
       61 ggctggatac cgaacacatt gatcgcggtc ttctgacaaa agaatggcga aaagggaatc
      121 gtgtttcacg catcactcgc acgacttccg gtgctaatgc tggcggcggg aacctcaccg
      181 atcgcggcga aggtttcgtc cacgatctga cgtcactgac gcgcgatgta gccactggcg
      241 tactggcccg ttcaatggac gtggacatct ataaccttca tccggcacac gctaaacgca
      301 ttgaggaaat tatcgctgaa aataaaccac ccttttctgt tttccgcgac aaattcatca
      361 ccatgcctgg cgggctggat tattcccgcg ccatcgtggt tgcgtccgtg aaagaagcac
      421 caattgggat cgaggtcatc cccgcacacg tcactgaata tctgaacaaa gtactgactg
      481 aaaccgatca tgccaaccct gatccggaaa tcgtggatat tgcctgcggt cgctcctctg
      541 ccccgatgcc gcagcgagta acagaagaag gaaaacagga cgatgaagaa aaaccacaac
      601 catctggcgc aatgacagat gaacaggcaa cggctgaaac agtggaaccg aatgcaactg
      661 aacatcatca gaacacgcag ccgctggatg ctcagtcaca ggtaaattct gttgatgaga
      721 aatatcagga actgcgggca gaactccatg aagcccggaa aaacattccg cccaaaaatc
      781 ctgtcgatgc agacaaatta ctggctgcct ctcgcggaga atttgttgaa gggattagcg
      841 acccgaatga ttcgaaatgg attcccggtc atcatatctc ctcaaatgaa gtcaaaaaaa
      901 cggaaaatga agtgcgacag actgaagata aacagcacca gaacagcgaa ccggaggagg
      961 gaacaccctg caatcagact ggcgaggata actgccctga ctgtggcgcg gtgatgggag
     1021 acgcaactta tcaggaaact tttgacgaaa aaaaaccaga tgaagcacag gaagaagagc
     1081 cgaagaaaac ggaaaaagct gatgatttgt taccggagaa cgctggcagc gatcagcata
     1141 atgatagcaa taatgaaact ggcgaagatg aactcaactg gaaaaaacag gtgttgattg
     1201 cggcggttta cggtttatgt gcgaatcctg cgtgcatagc ctatgcgcct gcaatccctg
     1261 acatcgcaat gatgattgcc aacaagcttg aaaattttgg agtgaccagt gatgagcgct
     1321 tacccgattt ttgatcgtat tgaagaaatg gcatgggctc gccactacca gcagatcgtt
     1381 cgtgaagaaa aagaaacgga actggcggac gacctggaaa aaggtctgcc tcagcacctg
     1441 tttgaatcgc tctgcatcga ccatttgcaa cgccacggtg ccaataaaca ggcaatcagc
     1501 cacgcatttg atgatgatgt tgagtttcag gagcgcatgg cagaacacat ccggtacatg
     1561 gctgaaacca ttgcccacca ccaggttgat attgatttgg aggactaaaa tctatggcgc
     1621 tttttcaacg agcaataaac acacaggcct atctaaaagc aggaattatg gggtttgctg
     1681 gcgacggcaa aacctacaca gcaagcgaac tggctattgg tctggtaatg cttatgcggc
     1741 aacggggaat tgaagctggt aaccgcccgg taatgtttct ggacacagaa acaggctctg
     1801 actgggttaa accccgcttt gatgcggaaa atatcgaatt atttacggca aaaacccgct
     1861 cgtttgttga tctgcttgaa gccattaatg aagcggaaag cagcggttcc gtaatgatca
     1921 tcgactccat cagtcacttc tggaccggat tatgcgacga gtatgccaga cgccgaaacc
     1981 gtaaacgagg actggaattt tcagactggg cgtggctcaa acaggagtgg cgacgcttta
     2041 ccgatcgatt tgttaacagt caggcgcaca ttattatgtg cgggcgtgca ggatatgagt
     2101 atgacttttt cgaaggtgat gatggcaaac gccagttggc aaaaaccggt ataaaaatga
     2161 aagcagaaac agaaaccggt tatgaaccct ccatcctgat ccagatggag aagcaaatga
     2221 acatagaaac cggtcaggtg tggcgcaccg ccagaatact aaaagatcgc tccacccgta
     2281 ttgatggaca ggtattttca aacccaacat ttaaaaactt tcttcctcat atcgagtttc
     2341 ttaacctggg aggtgaacac ctcggcatcg atacctcacg ggataacagt gaattatttg
     2401 ccaacgacgg tacaccgaca tggctaaaag aaaagc
//
LOCUS       DAETPZ010000232         2435 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.238, whole genome shotgun sequence.
ACCESSION   DAETPZ010000232 DAETPZ010000000
VERSION     DAETPZ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.238"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..215
                     /locus_tag="K0W59_004280"
     CDS             <1..215
                     /locus_tag="K0W59_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HBI3025013.1"
                     /translation="RSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLR
                     EVIEKLNDWPSNQVHELLPWNFSSVK"
     gene            complement(469..633)
                     /locus_tag="K0W59_004281"
     CDS             complement(469..633)
                     /locus_tag="K0W59_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3025014.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1534..1818
                     /locus_tag="K0W59_004282"
     CDS             1534..1818
                     /locus_tag="K0W59_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBI3025015.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1806..2291
                     /locus_tag="K0W59_004283"
     CDS             1806..2291
                     /locus_tag="K0W59_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI3025016.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSINHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          629 a          519 c          690 g          597 t
ORIGIN      
        1 tacgatcggt ggcggttgga agaaaaaatt atctcttttt cggctcagac aagggaggag
       61 aaagtgcggc gatcatctac agtctgctgg tcacctgcaa acagaacgaa gtggagccgg
      121 aggactggtt gcgcgaagtg atcgagaagc tcaatgactg gccgtcgaac caagtgcatg
      181 aactgctgcc ctggaacttc tcgtctgtaa aataatcctt acgctacgta cttctcgggg
      241 cgcttacctt caaaatgatg gacattattt ttgtggaacc ggaggtaaca gaccagacgg
      301 tttaaatgag ccggttactt taaacgggtg atggacagca cctcaaaagt tcagatggat
      361 tatatttgtc tgcaataccc cgggtatttt cgatttgcga aatggatgga acgacttgct
      421 caggggatag ctgatggagt tactgaagta ccgaaggacc actaaaaatc acaggccggg
      481 aatttcggcg tttccggcgt aggtgtgcca cgaatgcatc cagtggcgtg atgttggatg
      541 caatgctgta tgagagatga ggggcggcgt ccagacgcag ccttcaaacc gccttgagct
      601 gctgccgtta agagcggtgg tggtgagcgt caaggttaaa aggcgcagaa catgtgtcag
      661 gtaggtgttg cggagatgaa cgaaaggaac tgtcgatgaa gtgtcgaaag gaacctctag
      721 gcgatgtcga aaatgtgcta tctacgcagc gcatgacgag tccagcagat atctggtact
      781 ggctgggcgg catccggcat ataggcagca tgaacgcaac acaagctctc gtacggaacg
      841 tgggaacctg tcgtctcgat gctaagggag cggcgcggag cagaggcact gcaagcgcct
      901 gagtaccgat gcgagacaca ggggcagatg gcttcgtagt agtgaggaag tgcctgtaat
      961 gggcatggag cgaagggggt cagtcgttgg ggctggttta tcggtcaacc ggatagccgg
     1021 gaggagccca tgagtcagcc aaagccgttc gaggtctcta agtatgcggt ttggaaagca
     1081 taccagcggg tcaaagctaa tcgaggtgcg gcaggtgtcg atgggcagtc agtcgaggcg
     1141 tttgaggtta ccaatcgaag ctatacccgg taatggcgca ggttgacctg cacttggcca
     1201 agtggattgc ccgcaaacac aagcgagcgc gaggtagtct ggtgcgtgca ttcgagtggt
     1261 tactgcgaat tcgtcacgac tgcccgacgc tgtttgcaca ctggtgctta gcttacgata
     1321 cttgaatggt tcaacgacaa gagccgtatg acgcgagagt gtcacgtacg gttccgtggg
     1381 agcgtagggg ggcggtgccc ctgcgccacc cgatatcccc tttgagtgcg gttacgtcca
     1441 cagaggtaaa tgccgctgac gcggcatctg taatctgtta ctttgtgtat atcatttgct
     1501 atatattgtc aatatgctat tgttgtaaag caaatgatat acggaggctt tatgaaatca
     1561 ggtgttcagc tcaatctcag agcaagagaa tcacagcgga tactcattga tgctgctgca
     1621 gaaattcttc ataagtcgcg tacagatttc attctggaaa tggcctgtaa agctgctgaa
     1681 gatgttattc tcgaccggcg ggtgtttaat tttaatgaca ggcaatatga agagttcatt
     1741 gaaatgcttg atgctccggt tgcagatgat cctgccatcg aaaaattgct ggcaaggaaa
     1801 cctcaatggg atgtgtaact gcaccagaac ctttatcctc atttcatcag gtcgctgaat
     1861 ttgtcagcgg tgaagctgta cttgatgact ggttaaagca gaaggggcta aaaaatcagg
     1921 caatcggagc cacccggact tttgttgtat gcagaaaagg tacgcaacaa atagtcggtt
     1981 tttactcact ggccactggt agtatcaatc atacagaagc tacaggtaac cttcggcgca
     2041 atatgccaga ccccattcct gtaattatac ttgcacgcct ggctgtagat gtgtcatttc
     2101 gaggaaaagg gcttggcgcg gatttacttc atgatgcagt tcgtcgttgt tacagggttg
     2161 ccgaaaatat cggtgtgcgt gcaattatgg ttcatgcgct tacggaaaac gcaaaacagt
     2221 tttatattca tcatggtttt aagccatcaa aaactcaggt gcaaacattg tttcttaaac
     2281 tccctcaata gggagtaatg ccgatcagtt aaggatcagt tgaccgatcc agtggctgtg
     2341 taagaatccg gaaacgctca ctcgtttccg gatttttctg tgcacagtaa aagaccacct
     2401 cctgaggagg tggcttttca gaatgccccc tataa
//
LOCUS       DAETPZ010000233         2373 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.239, whole genome shotgun sequence.
ACCESSION   DAETPZ010000233 DAETPZ010000000
VERSION     DAETPZ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.239"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..823)
                     /locus_tag="K0W59_004284"
     CDS             complement(<1..823)
                     /locus_tag="K0W59_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBI3025017.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1424..1672)
                     /locus_tag="K0W59_004285"
     CDS             complement(1424..1672)
                     /locus_tag="K0W59_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBI3025018.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2027..>2373)
                     /locus_tag="K0W59_004286"
     CDS             complement(2027..>2373)
                     /locus_tag="K0W59_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBI3025019.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          611 a          439 c          575 g          748 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggcaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggttg tttttccatt
      721 cagtccaatg ggttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggcgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggagggg gcaatttttc cttaaggtat gaaaaaagga
     1081 gctgaagcta tataatagct attaatgcca ctggtatcag tgaatcatgt atataaaaaa
     1141 ctgtgttaag gaggctgtcc attagcggtt ttttattctt ctggaaccgc ggcatctttg
     1201 cgtatctttt tgtcttctca ccgtctggtc ggtgttttgc tgagactgct aatttcctgt
     1261 ctttgctagt gctgtactgg tactgcccaa tcatgattgg cgggggcggg cggagttgag
     1321 accgcagaca cgtcgtatgc aagaacgtgc tgcggctggc tggtgaactt ccgatagtgc
     1381 gagtattgaa tgatttccag ccgttactga ttttacgtgc taattagtga acaaaccact
     1441 cgtcagcaga ctcccaggta tctttcagag tttcctgaac aaaagtttta gctgaatctt
     1501 tatcggcggt gcgcgtaaca gaaaggccat cgttgctggt ggcttttacg atcacctcta
     1561 catcgtcata acgtttactg atgcgtcggg ttaattcttc ctttaacgca tccacagcac
     1621 cggttggcat tttagtcatt ttctctttgg ctatgcagat ttcaatacgc ataaaaatcc
     1681 ctctacactg tgtttttata cagtattatt tttaactgta tggataaaca gtgtcaagag
     1741 tgagggaaag tgattttttg gggcatgcat ggggcaaaaa tatttgttgt ggggcatttt
     1801 ggggcatgat tgggacactt tgttacatat gaactttgcc gattttcata tgacatggca
     1861 atccaacgct ttgattattc taaagaaaac acatgctctt gggcgttctt tagtgatttt
     1921 taaaaatccc gtatcacgca gttaaagtgg cgggcatact cttcaaggct ggtgatcccc
     1981 aaccgcaccc atttaggatg cgaccattgc ggtagaccga tgtaaatcat aacgcgtgaa
     2041 ggatctcttc cacgctacgc acacgggcga tgcgcgggta gatatgatta atgctgttat
     2101 tgtgttgctc ggcgctggcg gcactacagg cgtcttcggc aatcaccaga ttaaagccaa
     2161 gttcccaggc attgcgggcg gtggattcaa caccgatatt ggtcgagatc ccacataaca
     2221 ctattgtatc gatacctcgg cggcgcagtt gcaactccag atccgtaccg taaaacgcac
     2281 cccactgacg tttgatgatt tcgatatcac tgtcagttgc acctaatgca gctggatgtt
     2341 gccaccagtt ttcgggcaac actttagcgg ggg
//
LOCUS       DAETPZ010000234         2355 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.240, whole genome shotgun sequence.
ACCESSION   DAETPZ010000234 DAETPZ010000000
VERSION     DAETPZ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.240"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            746..2254
                     /gene="ltrA"
                     /locus_tag="K0W59_004287"
     CDS             746..2254
                     /gene="ltrA"
                     /locus_tag="K0W59_004287"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBI3025020.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYKF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          594 a          584 c          697 g          480 t
ORIGIN      
        1 cgcaggcgac cgcgtaaacc gtcagttcgt ggcagaacga cctgaccagc tgtgggtggc
       61 tgattttact tacggtgtgc ccggagttca gggcgggcat ggatgcttaa atgaaccgcg
      121 agtctgtctg gaatattgaa ccggtaactc acgatgagaa acccaacaat cccaccgggt
      181 gtgacggtgg agaacctgag cggcagtgac ctgcggcatg cccgcagggt gatgtaaccc
      241 gctgacaacg gggattgagg cgagatcact aagccgagat gatcctcaag gttaagtact
      301 gaaaggctga agaacatgaa cccgttaatc cgcctctgtg ggttgaaaac gtcaccacgg
      361 cctacgtgat ctgacaggcc gtgcaggagg aactggcagt gatacgtaag cactgccggt
      421 cgaaggtgtt ttgacatgta tgcgaaacac cggggcagca gcgtctatca cgcttgcgtt
      481 gctgacttct gccaacttgc ggcaagcaag gataaagagt gcgacgggca gcctcctcag
      541 tatgcctgag tccaggcagg taaaccgggg aaggtcagcg acggatgtta agggggcatg
      601 gctccgatga cgcgctgact ggcggagctt ccgtagtagt ccgcgatggg gaaagcccat
      661 tacatggcga agggaagcag tttgaatgtg tttgcgacgt gaattaactg gcctaacgag
      721 gtgaagacct ttgataatca gcgaaatgca acgcaagctt gccacatggg cagccaccga
      781 tccgtcccta cggattcaac ggctgctgcg tctgataaca caaccagaat ggctggctga
      841 agcggcgcgg atcacgcttt catcaaaggg ggcgcatacc cccggcgttg atggcgtgaa
      901 caaaacaatg ctacaggcca gactggctgt tgagctgcaa atcctcaggg atgaattact
      961 ctcaggccac taccagccct tgcccgccag acgggtttac atccctaaaa gcaacggcaa
     1021 actgcgacca ctgggtatcc ccgcgttgcg ggatcgtatt gttcagcggg ccatgctgat
     1081 ggcgatggag ccgatatggg agagtgattt tcatacgctc tcatatggct tccggcctga
     1141 gcgcagtgtc caccacgcga tccgcacggt gaaattacag ctcacagact gcggtgaaac
     1201 ccggggacgc tgggtgattg aaggcgacct gtccagttac ttcgacaccg tacatcatcg
     1261 actgctgatg aaggctgtac gccgcaggat cagtgacgca cgtttcatga ctctgctgtg
     1321 gaaaaccatc aaggcgggac atatcgatgt cggtctcttt cgggcggcca gtgaaggtgt
     1381 gccacagggc ggtgttatat cgccgctatt atcgaacatc atgctgaatg agttcgatca
     1441 atacctgcat gagcgctacc tgagcgggaa agccagaaaa gatcggtggt actggaataa
     1501 cagtatccaa cggggccgaa gtacggcggt cagagaaaac tggcagtgga aacccgcggt
     1561 ggcgtactgc cgctatgccg atgattttgt cctcatcgtc aaaggcacca aagcacaggc
     1621 ggaagccatc agggaggagt gtcggggtgt gctcgaaggc agtctgaaac tcaggctgaa
     1681 catggataag actaaaatca cccatgttaa tgacggcttt atctttctgg ggcacaggat
     1741 cattcgcaaa cgcagtcgtt atggcgagat gcgagtggtc tcaacgatcc cgcaggagaa
     1801 agccagaaac ttcgccgcat cgctgacagc actgttatca ggcaactaca gtgaaagcaa
     1861 agtcgatatg gctgaacaac tcaaccgaaa actgaaaggc tgggccatgt tctataagtt
     1921 cgttgatttt aaggccaaag tcttcagtta tatcgaccgt gtcgtgttct ggaagctggc
     1981 tcactggctg gcccgcaaat accgtacagg tatcgcttcc ctgatgaggt ggtggtgtaa
     2041 atcaccgaaa ccgggtcaga gcaaaacgtg ggttttattt ggtaaaacca atcacggcaa
     2101 gctcagcggc gaaatactgt accggttggt ggggcaaggc aagaagctgt tccgctggcg
     2161 gctacccgaa ggtaatccct atctgaggac ggagaccaga aacacgtata catcgcgctt
     2221 tacagaagtg gcaatggcgt tcgccagcat ttaaatggag agccggatgc gctgaaaggt
     2281 gcacgtccgg ttcggggagg agaggcaggg aaatagtccg actacgccct gcctcttact
     2341 ctactcagca catgg
//
LOCUS       DAETPZ010000235         2333 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.241, whole genome shotgun sequence.
ACCESSION   DAETPZ010000235 DAETPZ010000000
VERSION     DAETPZ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.241"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..219)
                     /locus_tag="K0W59_004288"
     CDS             complement(<1..219)
                     /locus_tag="K0W59_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBI3025021.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNL"
     gene            525..1307
                     /locus_tag="K0W59_004289"
     CDS             525..1307
                     /locus_tag="K0W59_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBI3025022.1"
                     /translation="METKQKERIRRLMELLKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVNKPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKAMNRGLRKILLARHALFDEVA
                     SASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(1309..1535)
                     /locus_tag="K0W59_004290"
                     /pseudo
     CDS             complement(1309..1535)
                     /locus_tag="K0W59_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
     gene            1777..2333
                     /locus_tag="K0W59_004291"
                     /pseudo
     CDS             1777..2333
                     /locus_tag="K0W59_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558297.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          645 a          559 c          503 g          626 t
ORIGIN      
        1 aagattacga atagtattat ccgcaaaaat atcatcacct actttagtca gcatcaggac
       61 ttttgaattc aatttagccg ccgccaccgc ttgattagca cctttcccac cacatccgat
      121 tttgaaggca ggtgcttcca gagtttctcc ttctttaggc atctgattag tgtaagtaat
      181 gagatccacc atattggaac caataactgc aatgtccatt tcactacctc ttataaactt
      241 tcgcataaca atggtattta aataacatta gcatgttact tttgcatcat ttgtgactga
      301 gatcgcgatt agcacatcaa cccgatgttt atttaataga cttccagtct catcactcag
      361 gccaacacta tctaatcata agcaacctaa caagattagt gcccaaaatt cagcagccta
      421 tacccttttc atttcaaagg ggcggtcgta tagtatggta atgaaaacaa tgtttactaa
      481 cgccaaaatg ttatttttat aacattctta cggagagaga gttgatggaa acgaagcaaa
      541 aagagcgtat ccgacgtttg atggaactgc ttaagaaaac cgacagaatc catttgaaag
      601 acgcagcgcg aatgctggaa gtttctgtaa tgactattcg tcgcgatctc catcaggaag
      661 atgaacctct gccactgacc ctactgggtg gctatattgt aatggtgaat aaacccgcgc
      721 catccatgcc agtaatccat gacgttccaa aaaatcatcg tgatgactta cctattgcaa
      781 ttctggctgc cggaatggtt aatgaaaatg atctgatctt ctttgataat ggccaggaga
      841 taccactcgt tataagcatg atcccggatg caatcacctt caccggcatc tgttactcac
      901 atcgcgtctt tgttgcgttg aatgaaaagc ctaatgtaac agcaatactt tgtggtggta
      961 cgtatcgtgc cagaagtgat gctttttacg atgccagtaa ctcttcgcca ttagactctc
     1021 tcaatccgcg aaaaatattt atttccgcca gcggtgtgca taatcacttt ggcgtcagct
     1081 ggtttaaccc tgaagatctt gccactaagc gtaaagcgat gaaccgtgga ctacggaaaa
     1141 ttttgctcgc ccgccacgcg ttgttcgatg aagtggcctc tgccagcctc gcaccgatct
     1201 ctgcatttga cgttctgatt agcgatcgtc cgttaccggc agattatgtt acgcactgcc
     1261 agaatggttc tgtaaagatc attacacctg attcagaaga cgaatgactt actgaaaaaa
     1321 caccacaatc ttgttaaaca tcgtcgggtt ggactgatta cgttgcactt tcaccacata
     1381 ttccagctta tctatttggc ttatcaccta ctccagacgc tggtcatcct tgaccagtag
     1441 ccagatatgg cttttgtcgc agtcctgaat cggcaggcag agaatgtctt caacgttaag
     1501 agcgcgacgg gcaaaagccc acaaaggtga gtcattacac ccggatggta aacaggcact
     1561 tccttgttgt agaagccgtt atattgtcca taatgtctgg gtttttaatg catactgggt
     1621 aacaaccgga cgaatcttcg aatatcactg gcgctgccag ccggtttgaa ttcatctcac
     1681 aaccctgcat agagcgaatc tcctgcctgc acgtcactct gaatcgccac gggtttaaca
     1741 gacacctcag agtcatttaa gatggcttaa agagaggtgc ccatgagcgg taagcgttat
     1801 cccgaagagt ttaaaactga agcagtcaaa caggttgttg atcgcggtta ttctgttgcc
     1861 agcgttgcaa cacgtctcga tatcaccacc cacagccttt atgccaggat aaagaagtac
     1921 ggtccggatt cttccactaa taaagaacag tcagatgctc aggccgagat ccgccgtctc
     1981 cagaaagagc tgaaacgggt taccgacgaa cgggacatat taaaaaaagc cgcggcgtac
     2041 ttcgcaaagc tgtccgactg aggtacgcct ttatccgtga caactcctgt tgctggcctg
     2101 ttcgcctgct ctgtcgggtg ctggatgttc atcccagtgg tttttacgcc tggcttcagc
     2161 agccgcattc acaacgccat caggcagacc tgagactgac aggacagatt aaacagttct
     2221 ggctggaatc gggatgcgtc tatggttatc gcaaaattca tctggatctg cgtaacagcg
     2281 ggcaacagtg cggagtaaac agagtctgga gactgatgaa acgtgtcgga ata
//
LOCUS       DAETPZ010000236         2302 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.242, whole genome shotgun sequence.
ACCESSION   DAETPZ010000236 DAETPZ010000000
VERSION     DAETPZ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.242"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..784)
                     /locus_tag="K0W59_004292"
     CDS             complement(<1..784)
                     /locus_tag="K0W59_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBI3025023.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMS"
     gene            1132..2004
                     /gene="rluF"
                     /locus_tag="K0W59_004293"
     CDS             1132..2004
                     /gene="rluF"
                     /locus_tag="K0W59_004293"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HBI3025024.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(2137..2302)
                     /gene="yjbD"
                     /locus_tag="K0W59_004294"
                     /pseudo
     CDS             complement(2137..2302)
                     /gene="yjbD"
                     /locus_tag="K0W59_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
BASE COUNT          562 a          589 c          577 g          574 t
ORIGIN      
        1 cgctcatatt cgtttcaacc attgcgccgt gaatatcaaa aaagcgcgag caaatatcgc
       61 cagcaacctg gcgggcattc aggtcgtcac tctcttcacc accataaaac gcatgccagt
      121 tagcgccgtc gcgaatggcc ggtgagccaa ttcccaccag ggcgacatcc agattatccc
      181 agtaggcaga gatggtttta aagtgctgag actgcatgat cccattacga attaatgggt
      241 tatccagcag agccggaaaa tccgcgagat gcgattcccc tttcagcttc gctgccgcgc
      301 tgtaggttaa tgtgtttaca tgatagcggc tttcgagttt accggacggt ccgccaataa
      361 ttggtacgca gattaactgc cgcgattgcc ccgcctgcgg caagttttca accagtgcgc
      421 tcaccgcgcg accccaggaa aaaccgacaa tatcgccagg ctccagcaag cgatccagca
      481 gttgcgcgcc gtgtaacccc atcatcgcca gttgagtctc ttcatcctca tcatttcccg
      541 acaccaccac aacgtctttc aggccaaact tttgcttcag ttgctgctcc agccagagat
      601 tttcgttgta gtcatagttg atggcgatgg tgaccattcc ctgatcgcgg cctcgtttaa
      661 gcaagcggct gatggtggtg cggtaaatac ccagttcgcg cgcgatttgc gcctgcgtca
      721 tatcctgttc gtaatagagt tgggcaatct tcacaatcaa acggatatcg tcactgtttt
      781 ccattgccgc ctcctggttc tgcacatttg tgcaaatacg ctttctcacc gcttgatctg
      841 atataacccc atcatgaggg cctttcaaag cgatttttca ctctttgcat ggggagaaat
      901 agcgctttct cttgcacatt tgtttctctg cgtagtagtc atcagcacag gtgtgcagcg
      961 attcgtaata tgttgattat ccttattttg ccaggcaaat ttgcatcgta cttccggaga
     1021 cacttttgtg ataaaaggcg tggcagatca catttaattt cgacgttgcc gcataaccct
     1081 gtttttcccc gctaagaggt acggtatatt ccgcgtttta caggagaatt tatgctgccc
     1141 gactcatcag tccgtttaaa taaatacatc agcgaaagcg gaatttgctc acgccgcgaa
     1201 gcggatcgct atatcgagca aggcaatgtg ttccttaatg gcaagcgagc caccattggc
     1261 gatcaggtga aacccggcga cattgtgaaa gtaaacggtc agttgattga gcctcgggaa
     1321 gccgaagatt tggtacttat cgccctgaac aagcccgtcg gtattgtcag caccaccgaa
     1381 gatggcgagc gcgataacat tgtcgatttc gttaaccaca gcaaacgcgt gttcccgatt
     1441 ggccgcctgg ataaagactc ccaggggctg attttcctca ccaatcacgg cgatctggtg
     1501 aataagatcc tccgtgcggg taacgatcat gagaaagagt atctggtgac ggtcgataaa
     1561 ccgattaccg atgaatttat tcgtggtatg ggggcggggg tgccgatcct cgggacggtg
     1621 acgaaaaagt gcaaagtcaa aaaagaagcg ccgtttgtct tccgcattac cctagtgcag
     1681 gggctgaacc gtcagatccg gcgcatgtgc gagcatttcg gctatgaagt gaaaaagctg
     1741 gaacgtacgc gcattatgaa cgtcagctta agcggcattc cgctggggga gtggcgcgat
     1801 ttaaccgacg atgagttaat cgacctcttt aagctcatcg aaaattcctc ttccgaggca
     1861 aaacctaaag cgaaggccaa accgaaaaca gcgggcatca aacgtccagt cgttaagatg
     1921 gaaaaaacgg cggaaaaagg cggtcgcccg gcgtccaacg gtaagcgttt tacctcgccg
     1981 gggcgtaaaa agaagggacg ctgattaacg cctcagactg atgaaaacgt ccaaaaagag
     2041 ctcggacagt cccctcgccc cttcggggag agggttaggg tgaggggaag attgtgtcca
     2101 cacagacgag tgaaaatctc aggcgttgat tgttcattag cgcctgccgc gcgtcgacgt
     2161 atttgctgac cagctaaatg acgcttcagg atccggttta taagcctgct ttttcttcaa
     2221 ttggcgggct tttttcgctt cagcctcacg ctcgaccatt aatttatcga tgtactctct
     2281 ttcttaatgc tgttggtttc gg
//
LOCUS       DAETPZ010000237         2227 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.243, whole genome shotgun sequence.
ACCESSION   DAETPZ010000237 DAETPZ010000000
VERSION     DAETPZ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.243"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(85..297)
                     /locus_tag="K0W59_004295"
                     /pseudo
     CDS             complement(85..297)
                     /locus_tag="K0W59_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..876
                     /locus_tag="K0W59_004296"
                     /pseudo
     CDS             572..876
                     /locus_tag="K0W59_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            1045..>2227
                     /locus_tag="K0W59_004297"
     CDS             1045..>2227
                     /locus_tag="K0W59_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3025025.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALEHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGFSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AA"
BASE COUNT          578 a          464 c          512 g          673 t
ORIGIN      
        1 cttattatgc ttccatcgga aacgatgatc tggcagcctg agttcacaga taaaacactc
       61 tccaggaaac ccggggcggt tcagtgaacc tcgatcggtg tggtggatta gcccggcagg
      121 tgggcgctgg ctcctgagcg ccataaacag ggctttaccc gtcaactctt tcgtcatgcg
      181 ctctcccatg gcgtaaccga caatttcgca cgtataaaca tctttgacgc cagcgagata
      241 cagccatcct tcccgggtgg caacatacgt tatatctgcg acccagacct gattaggggg
      301 gttggggagc aatggaacag taaacgccgt taagaagtca atttaaatag ccaaaaatta
      361 actagccaat ttacattgca ggttgtaatt tttaaccacc ttttttacga tagccaaatt
      421 acctataccc atcccggccc tgccaagcta tacccgctat cggcgatgaa aatgcttttg
      481 tgcgcggtcg gagtggtccc gacgagggtt ttacccgaag tcggggcgta tctccgcgtt
      541 agcgggccgt gagggccgct tacgagcgtg tatgagagac gtacagtggt tttgtccgct
      601 cacaccagta catacaggta cggaaataaa accagtcgaa atgccggtaa cgacgattac
      661 tcctgtctct gatgccggtg gactacaaga ttttatttac tggcgtcctg atgcggacag
      721 gactggtatg gaacctgttt atgtgatact taatgatcct ctggattccg ggcgatttac
      781 gcgtaaacaa ctaaataaaa atatcttaaa catgcaagta attttggtat tctcgataca
      841 agaataaata gtagtaacta ctatagttgc aaataatgtt aaaaacatct ctatgatcat
      901 ctttacattt ttcatgattg acaattgttt ttggttaaaa aaagacaact aatgagtgtg
      961 ataatgcaat actttgagtg attatataat gataagtaaa caggtttgcg acaattggtt
     1021 atcagtatta cagaaggatt aaatatgtca ttattcacag aacctaagga aattgaaaga
     1081 ttaccctctg aagagattga acgattatat cccgtcctgc gatacagggt ctttatcagc
     1141 attttcctcg gatatatggg gtattatttt gtccgaaaca ccaccagtgt gttatccggt
     1201 gtgctgcaca tgtctgcaac cgagatcggc attatctcct gtgccggttt tttatcctat
     1261 ggtatatcaa agtttgtttc cggacttatt tctgacagaa gtaattcaaa agtatttttg
     1321 tctcttggtt tattcctgtc cggactggta aattttctga taggatatat tcccgggatc
     1381 atcacatccg ttacgttatt ttcaacaatg tatttactta acggctggat ccaaggtatg
     1441 ggatatcctc ctggtgccaa aacactggtg ttctggtatg aacatcggga aaggatcacc
     1501 tgggccacac tgtggaattt atcgcacaat gtcgggggag ctctggcccc cgttctgatt
     1561 ggtttcagtt ttggtttttt cggcgacagt gctctggagc atgcccgggc cgcatttatc
     1621 ttcccaggag tactgtgtat ggcaatgagt gtgctgattt actttattca ggtggacaga
     1681 cctgtatcag tggggcttcc tcccattgaa gaatggaaag gtaacgtcgt ttcacacccg
     1741 gctaaaggcc gtgaacaagg tccgagactc tccattcctg atatcatcag aaaacatatc
     1801 atccgaaata ataaactcat ttactgctgt atctatggtt cgtttgttta tattctgaga
     1861 tacggtattg tttcctgggc tcctaagttt ttaagtgaca gtctggatgt cggtggtaag
     1921 gatatgggaa aactggcatc aatggggggg ttttctgttt ttgaaatcgg tggtgtggct
     1981 ggtatgcttc ttgccggata cctgtctgtc agactgttca gaaactcaaa accattaaca
     2041 aatactttat ttctggcact taccattatt ttattaatcg cgtactggta cgtgccatca
     2101 ggcaatgaat atctctggct aaactataca atccttattc tgttggggct ggcagtgtat
     2161 ggtccggtga tgtttattgg tctttattca atggagcttg ttcctaaaga agctgccgga
     2221 gccgcat
//
LOCUS       DAETPZ010000238         2136 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.244, whole genome shotgun sequence.
ACCESSION   DAETPZ010000238 DAETPZ010000000
VERSION     DAETPZ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.244"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            14..487
                     /locus_tag="K0W59_004298"
     CDS             14..487
                     /locus_tag="K0W59_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI3025026.1"
                     /translation="MRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVADPNLDELYQV
                     YSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGKGLAKKLALMA
                     MEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCEVRMLREL"
     gene            complement(533..949)
                     /gene="rraB"
                     /locus_tag="K0W59_004299"
     CDS             complement(533..949)
                     /gene="rraB"
                     /locus_tag="K0W59_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HBI3025027.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1111..2124
                     /gene="argF"
                     /locus_tag="K0W59_004300"
     CDS             1111..2124
                     /gene="argF"
                     /locus_tag="K0W59_004300"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBI3025028.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT          544 a          520 c          532 g          540 t
ORIGIN      
        1 gcaatcacct gtaatgcgtc gcctgacgct gcaggataat cctgctatcg ccagcgtcat
       61 ccgtcaggtt tacgcagagt atggtcttac cgccgataaa ggctacaccg ttgccgatcc
      121 gaatctcgac gaactgtatc aagtgtacag ccagcctggc catgcttatt gggtggttga
      181 atacaatggc gaagtggtcg gcggcggcgg gattgcgcca ttaacaggta gtgaagcgga
      241 catttgcgaa ctgcagaaaa tgtactttct tcccgctatt cgcggtaaag ggctagcgaa
      301 aaaactggcc ttaatggcga tggagcaggc tcgagagatg ggcttcaaac gctgctatct
      361 ggaaacgacc gcttttttaa aggaagccat tgcgctttat gagcatttgg gctttgagca
      421 tatcgactat gcgcttggct gcacgggcca tgtcgattgt gaagtgcgga tgctacgcga
      481 actctaaaaa aatgcccgct agtatgtgct atcgggcatt ttaatcaatt aattagtggc
      541 gaaccccgtc atcgtcttca tcgacaaaat cttcatcgtc gccatcttcg ccgttaggat
      601 cttcaaagta agtgccccaa ccgtcgtact caacgtcaaa tttctccgcc agcgtcatca
      661 gttgttcaac ctgggcatcg atcagatcgg cattcaacgc gcactcgctg aggatgtcgc
      721 agcaaatcac gatatcacca tcttcaactt ccagctcttc tggatcggtc acttcgtaac
      781 cgagtttaaa cgcttcaact gccgcttttt ccagagtttc cagatcgtct gcggaaagat
      841 ggtgttcgat ggtgtacagc gcgtccggat cgctgccatc ttccagtaat tcttcaataa
      901 tcaaacgtgt ttcttcacgc tgttcttcca gttgttccgg gtttgccatg gctcattcct
      961 cttaatgtcc tgccgatact cttattgtca cacaccactg acattgcctc cacctttgtg
     1021 acaaagattt atgctttaga cttgcaaatg aataatcata cacataaatt taattttaat
     1081 tcattgaggc gttagccaca ggagggatct atgtccgggt tttatcataa gcatttcctg
     1141 aaattactcg atttcacgcc agctgaactc aacagcctgc tgcagttagc cgcgaagctg
     1201 aaagctgata agaaaagcgg taaagaagaa gccaaactca ctggtaaaaa catcgcgctc
     1261 atcttcgaaa aagactcgac tcgtacccga tgctctttcg aagttgccgc atatgaccag
     1321 ggtgctcgcg ttacttatct tggcccaagc ggtagtcaga ttggtcataa agagtcgatt
     1381 aaagacactg cccgcgtgct tggtcgcatg tatgacggta ttcagtatcg cggctatggt
     1441 caggagattg tcgaaacact ggcggaatac gctggcgtgc cggtatggaa cggcctgacc
     1501 aatgagtttc accccacgca gttgctggcg gatctcctca ccatgcagga gcatttaccc
     1561 ggtaaagcat tcaacgaaat gacgctggtc tatgcaggtg acgcacgtaa caacatgggc
     1621 aattcgatgc tcgaagctgc ggcgcttacc ggtctggatt tgcgtctggt cgcgccacag
     1681 gcatgctggc cagaagctgc gctggttacg gaatgccgcg ccctggcaca acaaaatggc
     1741 gggaatatta cgctgacgga agatgtagct aaaggagttg aaggtgctga ctttatctat
     1801 accgatgtat gggtgtcgat gggtgaagca aaagaaaaat gggctgaacg cattgcatta
     1861 ctgcgtgatt atcaggtgaa cagcaagatg atgcagttga ccggtaaccc ggaggtcaaa
     1921 ttcctccact gcctgcccgc gtttcatgac gaccaaacga cgcttggcaa aaaaatggcg
     1981 gaagagtttg gcctacatgg cggaatggaa gtgactgatg aggtcttcga gtctgccgcc
     2041 agcattgtat ttgatcaggc ggaaaaccgc atgcatacca tcaaagcggt gatggtcgcg
     2101 acgctcagta aattaaataa ttaatatctc tataaa
//
LOCUS       DAETPZ010000239         2128 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.245, whole genome shotgun sequence.
ACCESSION   DAETPZ010000239 DAETPZ010000000
VERSION     DAETPZ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.245"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(246..443)
                     /locus_tag="K0W59_004301"
                     /pseudo
     CDS             complement(246..443)
                     /locus_tag="K0W59_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125062.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(575..2098)
                     /locus_tag="K0W59_004302"
     CDS             complement(575..2098)
                     /locus_tag="K0W59_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBI3025029.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          636 a          467 c          451 g          574 t
ORIGIN      
        1 atgacattga tcactttggg gtgactattt tgtttaacaa ataactcttc gacatcaaaa
       61 ttattaatag ttgccatggt aattatttcg gtgatatata aaaacaactg tgataacacc
      121 aaggttgaca actatttgcg caataattcc tttccttcaa tgatccaggc gttcactgcc
      181 agcaacccat tcagccgcat actgacagtg agaaactgaa agattattgc tgatccctgt
      241 ggctgacgag tctttctctt aaatcgatta accagattga tcgccagcca aacaagggca
      301 ttaagcaaat ttatcgtcac aagcacttct cccagcccgg tactgggttt tgcaataagg
      361 atgaccagga gggtgatcag cataaatgca atggaaaaga tgatatagag agtgaaataa
      421 tccagattat tactattgtt catgctacta atggcaatcc tgtcaaaact ttcaaacaat
      481 tatagcaaac aaaataatga acaatagtaa caagaggcaa cgagctaatt agctgtctat
      541 aattacaccg cactatggcc ttactgatca attttcactc aaccacaacc ttcaccttcg
      601 gtttagaaag gataattttg accaggtcga tgtatgtaac aggtttatag aaataataac
      661 cctgctgaaa tgtgatatta ttttggttca gatagccaag ttgttctttc gtttcgacac
      721 cttcagcgac gatactgatc gaaagtttac gcgccagatc caatacacaa tccagaattc
      781 gggttgattc tgggtctgcg tttacgcggc caacgaaact gtgatcaatt ttgatataat
      841 caatatgcag gtcatgaaga taagaaagcc ctgagtagcc agtaccgaaa tcatccagcg
      901 cgatgacaaa accattttca tgcagaatgt tcaaccgctg aaccagactt tcatcaacat
      961 tcaatggctc acgctcggtg acttccagaa caaggtttaa atcgcggcgg gtaaaactgt
     1021 cacggaaatt taaacactcg tcgacaaacg tcggcgaaat aatatgcgaa gcgctaaaat
     1081 taattccaat atgaaaacct tccggcagtt tactcgcgat agcgttcatc tgtctggcaa
     1141 cctgattcat caggctttgt gtaagcggaa cgattaatcc ggatttttca gcaagtggaa
     1201 taaatgccgc gggtgaaata tatccaccgt gaggttgttt ccagcgggct aacacctcaa
     1261 ctccccgcaa tgtcccttcc cgaccattta ccacaggttg gtaaaaaggg acgatctccc
     1321 cattatttat tgcccgtcgc aggatctctt caggcgttgc gcttttattg aaatatctat
     1381 caagcaggaa ggcagcggcg caggcgatta acagaataaa tattaaaatc ccaaagcctt
     1441 tatcgataag tctgtagaag ctaaagagtg gcggcgggtt aaagataata gtgaaatgat
     1501 attttgtgga gtgaaccttc tcggtatagg gataatgtcc attaagcgtc atcacatcac
     1561 ccgttggtcc aataatgtca tccgccacgc gtaatacata ccttacccct ttcaagggta
     1621 cattaagcgc cccgcgaata tgttgatcac ttatcgtaac caaaatgcgc gtatctgcaa
     1681 attggctctg atagagcagg ataggtaatc tattaacggt gtcgattgcc ggagccaaca
     1741 gtaaattact gtccgggaca acaggaatac gagacagcag gacccgattc ccaggcaggg
     1801 atgtacacca gactattccc tgtttgagaa tgatgattgt gcgcaggtga ggtttcagag
     1861 ctgcttcagt gccaagctga tattgctcct cgaggtcgca ttccttcccg gcaatgttaa
     1921 ccgctgtttg agtcgcctgt gacgcttctt caagtatatg attgatattg ttggcggcat
     1981 atcttgctcc cgccaggtac tctgcttttg cggaatacca gagctggatg ttcaaaatag
     2041 cgacaccggt aataaacaag catatcgcaa cgaggatggg tatgagtgta ttgcgcattt
     2101 gcttgtcccg gccaaagggt ttaataca
//
LOCUS       DAETPZ010000240         2088 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.246, whole genome shotgun sequence.
ACCESSION   DAETPZ010000240 DAETPZ010000000
VERSION     DAETPZ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.246"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(60..1025)
                     /locus_tag="K0W59_004303"
     CDS             complement(60..1025)
                     /locus_tag="K0W59_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBI3025030.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1878..>2088)
                     /locus_tag="K0W59_004304"
     CDS             complement(1878..>2088)
                     /locus_tag="K0W59_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3025031.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          575 a          548 c          437 g          528 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gtgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac ccccaaaaca agcacccccg aacggcttac cccaaccttt
     1381 tccacgcacc cgatcaccaa aacagggaat aaaaacaaaa ccctgtctta caggtggatg
     1441 gcctcacttc gtgaaaccat ccaccccgca taagtttttt catcggtggt gtcgtcaatc
     1501 actaaatcgg aaccccaaag gggaccccca cgatttagca tttgacgaga ttaactctcg
     1561 gagatggcat catgctttta acggacttta aaaaacggtg aacggatgcg gattttaatc
     1621 cgtgtctgtg aggcgttgag caacgagcaa agcgaggcgg tagaaagaga gccagcggca
     1681 ggctcaggac gataatccgg ggaaaatgta ccactcgcag agtgttaaca tttgacatcg
     1741 gattgtctat cctgcattgg tgtcgagttg gcaaggctga ggataatgct gaggataatg
     1801 caggatagac agagcactag agattgcatg caatctcgta cctatcacct cagcctgccg
     1861 ctggctcttt tccagaacta agaatgcgtt cttaaaaatc caaacttgat accgtttaaa
     1921 tctattttca caaaaagagt ttttaccctc tcgtttgttt tctgaatata taaaattaat
     1981 tcatcctcaa aaccttcgtg cggatgaaaa ccaaatgtgc caaaacttaa tccatcaata
     2041 agcaaaataa ggtttttaaa cctgtcagga atcactgctt cacttctt
//
LOCUS       DAETPZ010000241         2063 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.247, whole genome shotgun sequence.
ACCESSION   DAETPZ010000241 DAETPZ010000000
VERSION     DAETPZ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.247"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..124
                     /locus_tag="K0W59_004305"
     CDS             <1..124
                     /locus_tag="K0W59_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3025032.1"
                     /translation="CFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            complement(273..1727)
                     /gene="ospC2"
                     /locus_tag="K0W59_004306"
     CDS             complement(273..1727)
                     /gene="ospC2"
                     /locus_tag="K0W59_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HBI3025033.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDVAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFINNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          646 a          367 c          338 g          712 t
ORIGIN      
        1 gtgctttggg cggctgaaag aatatcgccg catcgccacg cgttacgaca aaacggcgag
       61 aaattacctg gcgatggtga aactgggctg catccgactc ttttatcaac gcttacgtaa
      121 ttaagggaca cagcctagtc tccaggattc ccggggcgtt caccacatgc aatgtgttgt
      181 tgcaggggaa cggtctgcac catcccctat atcgatcaat ataacaaaaa tgacaacgtc
      241 agattcaggg cacaacctaa atatatttta ggtcacctcc caaatcgttt gcctgatacc
      301 gctccatttt ttaataaaaa atcaattgtt tttgaatctt tgcttttcat tgcattgtca
      361 agcatagtgt ccccactatt tgctttttga aatcttttgt ttacatcaac caatccatta
      421 ttaataaaat attctagcac tttatagttg gcactatctt cactaagtaa atactcaaca
      481 tcatacagct cacaatacat attgttgttc attttctcca tctctgcgac atcttgtttt
      541 gtaaaattaa atttagatag taaataaagt gccatatcag attttgcatt gcctagagaa
      601 taagcaaggg cctgatgagc cattttttta tcagtcacct tattgttaat ttcttcataa
      661 ttggatgctt taatggcatc ctccaaagat atatctctga gtcttatctt tttgaagtta
      721 tctgtgctta gcattcttgg tatatgatac tcaagctgaa acacaaagtt tataattcta
      781 tccagactaa caggatcaat gtttttatta taacaaaact ctcggaatct ttgatcttta
      841 ctctttctta taaaatcaat aagatgcaac ccaattccaa gcctcatatc ttttgtatta
      901 aatattggca catcagtttt tccctgatta ccatgcacat acctgctaac ttcatccaca
      961 acctctttaa agttatctaa gaaaaatgat tggtgctcat ggtaaaaaac aggtggaata
     1021 gcattatcaa agtggtcggt taatgtcata tatccatatg gagagtcatg atcaatgata
     1081 tacgcattag caccaaaatc cacagtatga tgtgttttat ttagtgggag tgtttcttga
     1141 tgattggaaa tttcaacccc aaaaaaaaca aaatcgtggt tagctatttc ttcaatatcc
     1201 tcagagaatg tgtttttata tgtttgtata ttctcagact taattttatc aaaggactta
     1261 atattaagag tattatttga caggttagcg ttactttgat gtctgaagtt aaaatcagaa
     1321 ttaataataa tagagagcaa gtgacgttca tccgaagaaa gaggatgagt tttttgtatt
     1381 atttcagaaa gatagtgtct ggacacctta tcgaggtgct caagtgaccg aaatgatgac
     1441 cgtgctccag atggcgcttc aatagaaaga tttaatgatt tgaaactggt cccttgagag
     1501 aacattttac tataactttg cgctgctata gacttcctta aaaagctttt cacagtatca
     1561 gcacagtgat ctaaatgttt ttcacttatg ccagaagagg atgagtttgt tacgcatgat
     1621 atgttttttt gtaatgcttt tgtatctttg ttcggattta tttttccatc aacaagatca
     1681 atattgttct gaacattaat atgatttact gcttcaggta ttttcataaa acatccccca
     1741 ttatgtgcgt ttttatctgc agttatttag aataatatta tctaacttaa tattttcttg
     1801 tatgtattat aacgaacata ttttaatatg ttgtacgatt ttcactctga aatcccctga
     1861 gtttaatcac acttccttta ctgaaaggaa ttattgcaac agtattattt gtttttggat
     1921 atatatcttg cgccttgtta tgtagttaat gctcagaata tgatagataa aatagagacg
     1981 caaaacaggg catgctcata acatgatgat aacataaaaa attagtaaca ataaatgaaa
     2041 gaatcatggg cgctcaacaa aaa
//
LOCUS       DAETPZ010000242         2059 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.248, whole genome shotgun sequence.
ACCESSION   DAETPZ010000242 DAETPZ010000000
VERSION     DAETPZ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.248"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(89..1501)
                     /gene="pykF"
                     /locus_tag="K0W59_004307"
     CDS             complement(89..1501)
                     /gene="pykF"
                     /locus_tag="K0W59_004307"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HBI3025034.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          523 a          457 c          497 g          582 t
ORIGIN      
        1 tgatttttta ttgcttctgg ttatcgatta aataaaaaaa gcgcccatca gggcgcttcg
       61 atatacaaat taattcacaa aagcaatatt acaggacgtg aacagatgcg gtgttagtag
      121 tgccgctcgg taccagtgca ccagaaacca taactacgac gtcacctttg tgtgccagac
      181 cgctctgcag agccagttct ttacccagac ggtagaaatc atcagtagaa gtgatctctt
      241 taaccagctg cggcacaacg cctttgctca gtaccaactg atgagccgtt ttttcgttgg
      301 tggtcagtgc caggatggtg gcatccggga agtatttacg tactgcgcga gcagatttac
      361 cgccctgggt agcaaccacg atcagcggag catccagttt ttcagcagtt tcaacggcac
      421 cacggcatac cgcttcggtg atgcgcagtt tacggttgtc attgttgaac tcgagacggc
      481 tgttcatcac gcggtcggta cgttcgcaga tggtcgccat gatagaaacc gcttccagcg
      541 ggtatttacc ttttgcggat tcaccagaca gcatcactgc gtcagtaccg tcgaggatgg
      601 cgtttgcaac gtcaccggct tctgcgcgag tcgggcgtgg gtttttgatc atggaatcca
      661 gcatctgggt cgcagtgata acgactttac gtgcacggat acatttttcg atcatcatct
      721 tctgggcgaa gataacttct tctaccggga tttctacacc caggtcgcca cgcgcaacca
      781 tgatgccgtc agaggcttcg aggatttcgt cgaagttgtt gaggccttcc tggttttcga
      841 ttttggagat gatgtggatg ttttcgccgc cgtgcgcttt caggtgctca cggatttcga
      901 taacgtcaga acgcttacga ataaaggaag cagcaacaaa gtctacgcct ttttcgcaac
      961 caaagatcag gtcctgtttg tctttttcag ccagtgctgg cagagcaatg gaaacgccag
     1021 gcaggttcac acctttgttt tcgcccaggt caccgttgtt cagcacttta cagataactt
     1081 tgttaccttc aatggcggta acttccatac cgatcagacc atcgtcaacc agtacggtgt
     1141 tgccaacaga caggtcagta gtgaaacctt catacgttac cgcaaccatt tcgctgttgc
     1201 cgataacaga tttatcagtg gtgaaagtaa aggtctgacc agctttcaga gaaacatcgt
     1261 taccgccttc cagtttcatg gtgcggattt ccggaccttt ggtatcaagc aggatagcgg
     1321 cggttttacc agttttgctc atcacgttgc gcagattctg aatgcgctga ccgtgttctg
     1381 catagtcacc atgagagaag ttcagacgca taacgttcat gccagcgtcc agcattttag
     1441 ctaacatctc ttcagattcg gttttcggtc cgatggtgca aacaattttg gtctttttca
     1501 tgacagtctt agtctttaag ttgagaagga tgggagaaac ttgctttctg ggcgcactgc
     1561 cggaaagcga atccggtatt acgaaagtgg tggtgcctcg ctctaaggat aggtgacatc
     1621 gaaaaagcgt gcagaggaat gtgtgcttgt gtctcagccc aacgtgagtg cctgtttttg
     1681 agtcgttgag ttctacagtc caaagtgctg aaaccattca agagtcaatt ggcgcgcatt
     1741 atacgctgaa attaatccaa aaggaaaaca aaaacagcgt ttcaaaaaat atacaacttg
     1801 ttagacaata aattgttatc aaagatttaa cgttccaggg aaaaggttac gcaaaaactt
     1861 cgctgtttgc gcaacgtctg gtgccgaagt tgtcagtgtg aggataagtg aaagttcatc
     1921 cggtgtataa atatatcatc gctcaattgc tacagttaaa ataatttatc gcgttatttg
     1981 tctgattttg tttgattttt gtttaattgg ttagattgat atatgctgat agttggcatg
     2041 ataaataggt ataaagaat
//
LOCUS       DAETPZ010000243         2038 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.249, whole genome shotgun sequence.
ACCESSION   DAETPZ010000243 DAETPZ010000000
VERSION     DAETPZ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.249"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(49..318)
                     /locus_tag="K0W59_004308"
     CDS             complement(49..318)
                     /locus_tag="K0W59_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBI3025035.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            375..1043
                     /locus_tag="K0W59_004309"
     CDS             375..1043
                     /locus_tag="K0W59_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBI3025036.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1359..>2038
                     /locus_tag="K0W59_004310"
     CDS             1359..>2038
                     /locus_tag="K0W59_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBI3025037.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPEFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          612 a          444 c          381 g          601 t
ORIGIN      
        1 accgcagcac gtcctgcata cgtcgtgtct gcggtttttc tttttcgctt acattgtgtc
       61 tggttcttcc ggccactcaa tatcaggtgc agttgatgta tcaacacggt tcagcaacac
      121 ccgatacttc ttccaggctt ccagcaacga gttttcttcc tccgttgcga tctccagatc
      181 tacagcatcc tgaagtggcg caatatgctc actgaatccc tggatgtaga actgtgtggt
      241 gacggtcttc cagccattcg gctcctgctg tatcgaagca taccaggcta tttcaatatc
      301 gctatgctgc ggcagcattt aaccccttgt aattcatcgc cataattgat ttaattcaca
      361 aataaaacta taatatggtg aaattaatga aaaaaaacac agatgatggg gctaaaattt
      421 acacaccact taccctaaag ctttatgact ggtgggtttt gggagtatca aatcggcttg
      481 catggggatg tcctacaaag gaacaccttc ttccacactt tctggaacat ttaggtaaca
      541 accatctgga tattggtgtt ggaactgggt tttaccttac tcacgtacct gagagtagtc
      601 tgatatcttt aatggatttg aacgaagcta gcctgaacgc ggcatctaca agggctgggg
      661 aatcaaaaat taaacataaa attagccatg atgtttttga accttatcct gcggcgttac
      721 atggtcaatt tgattccatt tccatgtttt accttcttca ctgcctgcct ggaaatatat
      781 ctacaaaaag ctgtgtaata cgcaatgcgg cgcaggcctt aactgatgat ggaactctat
      841 acggagccac aattcttggc gatggagttg tgcacaatag cttcggtcaa aaactgatgc
      901 gcatttacaa tcagaaaggc atcttttcaa acacaaaaga ttccgaagaa ggcttaacac
      961 atatactctc agagcatttc gagaatgtta aaaccaaggt tcaaggtact gtagtaatgt
     1021 tttccgcttc agggaaaaaa tagcatccaa ccgcagcacg ttcttgctta agacgtgcta
     1081 cggtttcctt tacgatttgg ggttgcgact ttacctctat tgataatgca ttccggcaga
     1141 acgttcaaat atgaacgtac gatatttaac taaccgaaaa acaaaatata gcacaggcga
     1201 gacatttacc atctaagaaa atagtatcgt ttttttacag ttaaatcaac atcacttcct
     1261 taaaatgaaa acaataaatt tcaatcctga aacattttat ttcagcttat tattccccag
     1321 tatcacattt tatcaggata tccttctgcg aggttattat gcttcctgta aataatcccc
     1381 ccctatccac tggaaacgtc tctttttaca gaactacatc aatcgacaat gttcacaata
     1441 attatctctc cgaatgggtt gaatggacta aaaacagcat ttccggagaa aacagggaaa
     1501 ctgcttttac ccggctccaa ttatgtctgg agaacagtga aacatcgttg gacttatctt
     1561 gtttaggtct cagatctcta ccacgattgc ctgacaatct tgatgaaatt aatgtaagca
     1621 ataaccaact atcaatgctc cccgagctac caagggcatt gaaagagctg aatgcaagca
     1681 gtaatcaatt atctgcactt cctgaattac cagtgtcgct ggaatatata aatgtgagtg
     1741 ataaccattt gttcgcactt cctgaattac ctgcgtcact agaatatatt aatgtaagtg
     1801 acaatcacct gtctgtactt ccgaggttac caatgtcatt ggaattactt gatgcagcca
     1861 gaaatgcttt ggaagtaata ccagagtttc cagaaagaga tgatcatatt ataagaatat
     1921 tctggcttaa tcagaaccgg atcacggcaa ttccggaaag catacttggc ctcagttctg
     1981 atagcgttgt caatcttaga gaaaatcaac tatctcccag aataatgcaa actttgtt
//
LOCUS       DAETPZ010000244         2034 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.250, whole genome shotgun sequence.
ACCESSION   DAETPZ010000244 DAETPZ010000000
VERSION     DAETPZ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.250"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..362)
                     /locus_tag="K0W59_004311"
     CDS             complement(<1..362)
                     /locus_tag="K0W59_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3025038.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQPVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /locus_tag="K0W59_004312"
     CDS             complement(429..1643)
                     /locus_tag="K0W59_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HBI3025039.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVMMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          634 a          492 c          414 g          494 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactggc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt gcgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcat gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgactactac catacaagag agaaaaagcg attattcaac cgtgttcaca
     1741 ttaaaaacaa aaatttccac cgaggagaaa aaataccctc aaaaaccacc tgaataatta
     1801 accattattg ttaaaacaac acgttaaata atcacataac aaactccatt aatcattgaa
     1861 aatgagcaca gaaatagaaa ttaattatat tcaattaatt aaaatgtgta ttgcttgatg
     1921 tatttaaaaa tattattttc atggtgagag tggaggtaaa gaagacacaa taataaaaag
     1981 gaatgtgaca aataatggtt ctggttgggc gttaaattta aggtcaccag aatg
//
LOCUS       DAETPZ010000245         2028 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.251, whole genome shotgun sequence.
ACCESSION   DAETPZ010000245 DAETPZ010000000
VERSION     DAETPZ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.251"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            269..406
                     /locus_tag="K0W59_004313"
     CDS             269..406
                     /locus_tag="K0W59_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001437041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase mbeA domain protein"
                     /protein_id="HBI3025040.1"
                     /translation="MEKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVISFQGKEWVHLK
                     E"
     gene            413..589
                     /locus_tag="K0W59_004314"
     CDS             413..589
                     /locus_tag="K0W59_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HBI3025041.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQV"
     gene            complement(948..1415)
                     /locus_tag="K0W59_004315"
     CDS             complement(948..1415)
                     /locus_tag="K0W59_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3025042.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQQLQIQEVCNSTLWETAKQQLLQESDGRYFAINSKHL
                     DFPGSGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFSHNLT"
BASE COUNT          525 a          407 c          581 g          515 t
ORIGIN      
        1 caaggctgtg acatcaaacg agacgaaaat caaagacgct atagccctgt tcacagcttc
       61 gacagaggaa tcactgaaaa aacaccggga aggggtgaaa gaggcgatga tgcagcacag
      121 gaaggacgtg ttaaagctgg cagggaatac gggcatgatg ttactgggga tggtcatttt
      181 cctgtttacc gtgagtggcg ggacgctctg gtatcttggc gggatgatac aggcgaacct
      241 ggaagaaatc aggaagcaga acgagagtct ggagaagctg aacgcgaaga catggggcgt
      301 ggagtttgtg caggacggga acaggaaatt tctggtcctt ccttacggga agtcagcaga
      361 ggtgatttcc tttcagggga aggaatgggt acatctgaag gagtgacaca gagtgacaga
      421 gctggaaaca catttgctga acgccttaga gcagctgcaa caggactata tgcagcggct
      481 gagcgaatgg gagagcgcct tcgtggaatt gcagaaaatg ttttcgctta cgcaacggga
      541 caacgcgatg ctgaacgagc gggtcatgca gttgagtcag caggtgtagc acttgagcga
      601 gcagacagaa cgcttgagcc agttatacag cgagaactgg agataagaga ggaacggctg
      661 atacaggagc gcgaacatgc gttatccctg gaacgggagc gtcagccgga aatacaggaa
      721 cgcacgctgg atggtccttc gctgggatgg tgaaaccatg aaaaatggca gcttcagtgg
      781 attaggaggg gttaatgtgg cctgtaccat ctggttgcat aggtattcat gcggttaaaa
      841 tttatcaggc gcgatcgcgg cagtttttcg ggtggtttgt tgtcattttt atctgtctgc
      901 tgccgtgatc gcaccaaacg cgtttcagtg gtgcgggcaa taagatatta cgttaaattg
      961 tgagagaatg gtccccatat actgacttta tcatacggat agggttggta gtgggtcgat
     1021 tgggtttcgg cattgtttcc gttaatgcca attgcatttt tgtatttgat gcaacagaag
     1081 gcgtgagatt ctcctgagcc tggaaagtct aagtgtttag aattgatggc aaaatatctt
     1141 ccgtccgatt cttgaagtag ttgttgtttg gctgtctccc aaagggtgct gttacaaact
     1201 tcttgaattt gtagttgttg caggactctt attactgcgt tgtcatgttc caattcttta
     1261 ggggagtgta ttattctctc ttttaataag ctgtttacca caggatttag ttgttgtcta
     1321 aagcacgcag ataatgccat tgctacacaa ttttgttccc gaggaagcat gtttggcggg
     1381 gtatgccgtg tttgatgtga tatgtctatt ctcatagaag gtctctggct tatagcatca
     1441 acaaatacgg ctataaaaca aaattttcat agagtgattc aagatcttct tgagatcttt
     1501 tttggcggtg cgtaatctgc tgcctgcaaa caaaaaaacc accgttatca acggtggttt
     1561 gtttgccgga atctagagct accaactctt tcttccgaag gtaactggct tggtggagca
     1621 cagataccaa atgctgtcct tctagtgtag ccgcagttag gccaccactt caagaactct
     1681 taatatctca atccacctta tccagttacc aatggatgct gccagtggcg ttaagtcgtg
     1741 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1801 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacca
     1861 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1921 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1981 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcg
//
LOCUS       DAETPZ010000246         1985 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.252, whole genome shotgun sequence.
ACCESSION   DAETPZ010000246 DAETPZ010000000
VERSION     DAETPZ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.252"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..557)
                     /locus_tag="K0W59_004316"
     CDS             complement(<1..557)
                     /locus_tag="K0W59_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBI3025043.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPLSLPMELTPSCNEFYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
     gene            complement(946..1212)
                     /gene="ospE1"
                     /locus_tag="K0W59_004317"
     CDS             complement(946..1212)
                     /gene="ospE1"
                     /locus_tag="K0W59_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HBI3025044.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(1321..1451)
                     /locus_tag="K0W59_004318"
                     /pseudo
     CDS             complement(1321..1451)
                     /locus_tag="K0W59_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1449..1544)
                     /locus_tag="K0W59_004319"
                     /pseudo
     CDS             complement(1449..1544)
                     /locus_tag="K0W59_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          565 a          339 c          420 g          661 t
ORIGIN      
        1 atgctgtcta atctgtttcc agaaactacc agtaatttta gttccggtgg caatagtggc
       61 agaatatgca atcggttatt atgaacccgt aaatgagtta gattttcagg aagaaatggt
      121 aattctttta tcttgttatc acccgcatta agctcttcta acgtctcggg taaggcaggt
      181 aagctttcca gttgattaaa ttgagcatgt attaccttaa gcattggtgg taaatcaggg
      241 agatgtgtta ataggttttt tcttatttcc agtgttgtta tctgaggcgg taaatctggt
      301 aatgttttta aatctaactc agataaattt aattctgcct cttgattttg taaacatatt
      361 ttcagcctat tgaaggcgat attgcgttgc tcgcctgggg taccgttctt ttcccattcg
      421 ctccagactt ttaaataaaa ttcattacaa gatggagtca gttccatagg taaagataat
      481 ggagttaacg agtatatatt gtttatttga tgcataatgc cagaaccgat tgcctgtata
      541 ttggttgatt taatcattat aaccagaatt cttatcaacg ataagaattc tagtgaaata
      601 gaatatggct tattaatttt aaaacagttt gctttgtacc ctttctccgg gtattgcttt
      661 ccccctaagc cgtctatggg aaggagtctg ctgttattcc tgagaatggt aattcagcca
      721 taaaaaggta tttaaggtca ttgcgtctct ccctatactc gaataatggt gaagaaagca
      781 tgatgaatat atcacgataa cgttcagtac gtatcgagga tagatggagt tactcctctc
      841 tgaaatagaa ttactgctct atttttttct gttttcagaa gttattagta acaaaccctg
      901 atatattttt ttgaaaatgg agcttaaacg agtctgaaat taacttcaga aatcagaata
      961 ccgttggctt tgtttttcat caattccggc ttcatttatc tttgtcaacc attgcatttc
     1021 tgtagcagtg aatttcaacg ttttcgtccg gctggcagca ggtatttgta attcgtatat
     1081 agctgctttt ttattgaaaa cagtatatcc atctgtagta acagtagaag ataataatac
     1141 aggatttgat acttcaagga tgatattttc ttgtttttgt ggaaggcaag ggaatatagt
     1201 ttgtgtaagc atattacttc cttatgttat attttggtaa aaaatttatt tttttacatt
     1261 atagtcggct aaatgaagtt tactgtaaaa aaacgatact ttttcgggtg gcgcttccaa
     1321 tcagtagaat atgtgttttt caatgaagga gccgatgact tttcgtggat ttccacggaa
     1381 cgcgagaagc agattgtttt acgtgccagc cgcttgatgc gggtgcggag ggtcaggttg
     1441 ttacgttcaa gatatagcat gtgctggatg aagatccctt ttctggtcac ctgtttatct
     1501 ttcgtggccg acggggtgac actgttaaaa tgtaatgaac gcatcccaca caattaaaca
     1561 cggagtagta tgatggttaa aagggtgaaa ctggcaggtg tggagcctcg cagctttccg
     1621 gcatcatact gcccaatacg ggatgcataa agcgtcctca gactggagac tccacaaatg
     1681 aaatatacac cggttggcgt tgatatggca aaacatgtca ttcagattca cttcatcaat
     1741 gagcacatgt cagcgccagt gatataagac ggtaatgggg atgcggacga tttcctggac
     1801 tctcagaaga tgaaacatgt ttactgaaga acaaaaaatt cgcgctattg agctttattt
     1861 caaatatggt aagaagcttg aggtgtactg gcaatagcgg acaccaccat ttgttctttt
     1921 tctaagcagt catctgataa tattttatcc tgaaggctgc cggggagata ttccccagac
     1981 gagag
//
LOCUS       DAETPZ010000247         1979 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.253, whole genome shotgun sequence.
ACCESSION   DAETPZ010000247 DAETPZ010000000
VERSION     DAETPZ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.253"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="K0W59_004320"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="K0W59_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HBI3025045.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
     gene            1494..>1979
                     /locus_tag="K0W59_004321"
     CDS             1494..>1979
                     /locus_tag="K0W59_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBI3025046.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VL"
BASE COUNT          563 a          341 c          478 g          597 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg
     1261 tcagggatta aaagatgaac gtaaatttat tcaacgcatt aattttaaag ggttttattg
     1321 tttgttgacg aaaacaggaa tcatgttcgg tctcttttta tctgttaaaa gccagaagca
     1381 tttccttcgc tgactttata gtcaaccata acacacactc tactgtctga gtccagcgtt
     1441 ttttaacatt cttgttaaga ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac
     1501 agcctgcgcc cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg
     1561 gcgatataca cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc
     1621 cgtggcgaga tttacttatc tcagtgggag acgttatcga tcgtgggccg caaagtttac
     1681 gttgtctgca gttactggaa caacattggg ttcgtgcggt aagaggcaat catgaacaga
     1741 tggcgatgga tgcgctggca ttccagcaga tgtctttgtg gttgatgaat ggcggcgact
     1801 ggtttattgc gctggcagat aatcaacaga aacaagcgaa aacggcgctg gaaaaatgtc
     1861 agcatttgcc ctttattctt gaagtacaca gtcgcaccgg caagcatgtt attgctcatg
     1921 ccgattatcc agatgatgtt tatgaatggc aaaaggacgt tgatttgcat caggtcttg
//
LOCUS       DAETPZ010000248         1931 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.254, whole genome shotgun sequence.
ACCESSION   DAETPZ010000248 DAETPZ010000000
VERSION     DAETPZ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.254"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(854..1327)
                     /locus_tag="K0W59_004322"
                     /pseudo
     CDS             complement(854..1327)
                     /locus_tag="K0W59_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1255..1621)
                     /locus_tag="K0W59_004323"
                     /pseudo
     CDS             complement(1255..1621)
                     /locus_tag="K0W59_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1623..1931)
                     /locus_tag="K0W59_004324"
                     /pseudo
     CDS             complement(1623..1931)
                     /locus_tag="K0W59_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          428 a          538 c          465 g          500 t
ORIGIN      
        1 tcgtcaaaaa aatcatcttc gtgaagtttt acgacatagc ggaggagttt gcttctttga
       61 ggtgcaggtt ttcgtttgcg tggtttagct gaacgggcca tataactacc tgaaagacaa
      121 tgatattgtc tgtttttata acggtaatca caggccatga caagccgcag ccgtcaggcc
      181 gcctacttgg ggcggtttag aacgcttatt tagtaccaga tcttacttat aataaagggg
      241 cttggcagtt tggataaact attgtcctta ttccactaat acaaactttt tgtaaagtat
      301 attaaatata cttcatttca tactgaaatc cccggaagta ttcaatcaaa caattacacc
      361 ataaaaggaa gagggggccc caattaagtt gtcaagcatg tttactgcgc aatcgcttgg
      421 tgctccgtcc ctgtaccccg cattggtact ctggcgctca tggggcttcc acttgcgctt
      481 ctcccttcac atcggggtga caggtccacg ttccccacaa gagcctgaat cgacttcacg
      541 ccgcttttat gccggatgcc gcgcaggcag taaacatatc ccacaggaac gactccacca
      601 ttgttttgac atcatctata tgctttcgac acatcatcag cggttcactt tcgttcgtct
      661 cttcgaacca tacttgatcg gctcacgtcc gaccttttcc catctcgctc accaccacat
      721 ctcttaaatg cagcagcagt ggatggtttg gtgcctgcgc ctgcacgccg acaccgaggg
      781 gccttccctc atctcttgtg ctgcatggct gcacagggac tcacgtcccc aggacgcgcc
      841 ttcgtggcac acagtcatcg gcatagcaac agtaagcaac ggcaggtttc cattgccagt
      901 tttctttgac agcagtactt cggcctcgct ggatactgtg attccagtac cagcgatcct
      961 ttctggcttt tccactcagg tagcgtttat tcagatactg gtcgaactca ttcagtatga
     1021 tgttcgacag caacggcgag atcacaccgc cctgtggcat actttcactg accgcccgga
     1081 agagaccaac atcaacacgg cgttttccac agcagtctca taaaacgtgc atcactgatc
     1141 ctgcggcaga cggctttcat cagcagccgg tgatgtactg tgtcgaagta gctggacagg
     1201 tcaccttcaa taacccggcg ccccgtgttt ccccgcagtc ggtgagctgt aatagtacgt
     1261 gatgtctccc gcccacttct ggttcgggcc actggcgtaa aaatcctgcg tcagcagatt
     1321 ttctgataca ggcaggccgt gtgcacggta actgatcggg ctgaacttct gggaggcttt
     1381 tgcgctcagt tcctgacggt gcagactggc tgccacggtt tttacgttaa agtggtaacc
     1441 agagcacaca gttcatccgt caggcgtggg gcaccgtaac gctggactgg gtaaaagcct
     1501 tgcggacaac gctgtcgcag tgctgacgga actgctgacg tgtgtttatc cttgtccgcc
     1561 gctgacacca cgtataccag ccgctgcggg ccacccggag tacgcggcac attgccttga
     1621 tgacggttta cgcggtcgcc tgcggcaacg gctttccggc tgacggtagt gtggaccttt
     1681 ttaccccgga gaacaccggc aagccccata accgccatga gacgtgccac agtgcatctg
     1741 gccactctga taccttcccg taacaactga tgccagactt tacgcacagc gtatacctga
     1801 tgattctcat cgtatacgcg cagtatctct ttcttcagcc agtcatcgcg ctgcgcacgg
     1861 gcactgcgtt tatcatgatg atgtcgctgt tgctgacagt ggtaatacgt tgacggggca
     1921 atatgcagtt c
//
LOCUS       DAETPZ010000249         1889 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.255, whole genome shotgun sequence.
ACCESSION   DAETPZ010000249 DAETPZ010000000
VERSION     DAETPZ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.255"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..66)
                     /locus_tag="K0W59_004325"
     CDS             complement(<1..66)
                     /locus_tag="K0W59_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3025047.1"
                     /translation="MMMELQHQRLMALAGQLQLESL"
     gene            complement(63..419)
                     /locus_tag="K0W59_004326"
                     /pseudo
     CDS             complement(63..419)
                     /locus_tag="K0W59_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            499..1692
                     /locus_tag="K0W59_004327"
     CDS             499..1692
                     /locus_tag="K0W59_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HBI3025048.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            complement(1718..1889)
                     /locus_tag="K0W59_004328"
                     /pseudo
     CDS             complement(1718..1889)
                     /locus_tag="K0W59_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          514 a          433 c          466 g          476 t
ORIGIN      
        1 aaggctttcc agttgcaact gcccggcgag cgccatcagt cgttgatgtt gcagttccat
       61 catcacgcca ctcctctgca gaatgagtcg tagatggaga gtggatgatg ctgggggggg
      121 ttgtcgaagc tcaccagatt ttcaccagga tgcacgtcat actctttttt ctccggaggc
      181 agtgccagca tggactgctg ctcttcgagc cagcgatcac agggacgggc ctggattgtt
      241 tcatgctttc gttggttagc gacatcgtgc agccagcgca gaccgtggcg gctggctgtt
      301 tcaacatcga cagtgctccc catcgggcgc aggcgagtca ttagtgggat gtaaaaactg
      361 ttacgggtgt actgcaccat ccgttccacc ttacctttag tctgtgccct gaagggctga
      421 ggaatcccca gaaaacgaga caaacatagc attatgtgat ctactgattt gtgccaacaa
      481 ttcaatgaga tcactgctat gcctgttcat aaagtatgtc agaatttctt ccgggatgcc
      541 ttagcaccat ttcatcagta ccgacaaaat gccctgatgg atgccacaat ggcgttaatc
      601 aacggagcat ctctgacgca gaccagtatc ggacgttttt tgccgggtaa tgcacaagtt
      661 aagaataaga tcaaacgtat tgatcgcctc atgggtaatg aggcgctcca tcgcgatatt
      721 ccaatgattt tcaggaatat cacttcaatg ctgacacggc agttatcttt atgtgtaata
      781 gccgtagact ggagtggcta tccctcccag gagcatcatg ttcttcgtgc cagccttctg
      841 tgtgacgggc gttccattcc gttattgagt aaggttgttc cttctgaaaa acaaaataat
      901 ccattgatac aacatgattt ccttgattcc cttgctcaat ccctgccccc cgatgccagg
      961 gttattattg tcacggatgc tggatttcag agcgcatggt ttcaccatat tacttctctg
     1021 ggctgggatt ttattggtcg gatcagaaat aacgttcagt attgcctcga taacgcgcct
     1081 gaaagatggt taaaagtgtc ggatagccct gaatgtaaaa caccggaata tatgggggct
     1141 ggaaggctgg ttaaagaaag gaaaaaaagc atccgggggc atttttacac ctataagaaa
     1201 tcggcaaaag ggcgaaaaaa gaaacgttcg aaaggtcaga gtggtttaaa caaaacagac
     1261 aaagaacaga gcaaatcagc aaaagaagcc tggttgatct tcagcagtac aaatgacttc
     1321 agagcgcgag aaatcatcaa gttatacagt cggcgaatgc agatagagca aaactttcgt
     1381 gatgaaaaga acgggcgctt cggatttggt cttcgggcca gcaaaagtcg ttcaacagga
     1441 agaattctgg ttctgagtct gctggcaacg ttgagcacta tcgtaatgtg gttactcggt
     1501 tatcacgctg aaaataaagg gttacacctg aaatatcagg ctaacagcat taaatcccgg
     1561 cgggttatct catatctgac gttagcgaag aatgttcttc gacactcgcc acttatttta
     1621 agacgaacag tactgagcac agttctaaac catctgtcca gaacctaccg aaatatggtg
     1681 ctggtttatt agcagtaatt ttgtggggat ccctcagcct gaaggggcga cacagtcggg
     1741 gagagaagcc catctccttg ccgaactgcc acagcgaagg atggaaccgg tgctgaccgg
     1801 tctgatatgc gtcacgttgc agaaccacag ttttcatatt gtcatacaac acttcgcgcg
     1861 gcacaccacc aaagaagcgg aacgcatta
//
LOCUS       DAETPZ010000250         1870 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.256, whole genome shotgun sequence.
ACCESSION   DAETPZ010000250 DAETPZ010000000
VERSION     DAETPZ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.256"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            28..1870
                     /gene="cyoB"
                     /locus_tag="K0W59_004329"
                     /pseudo
     CDS             28..1870
                     /gene="cyoB"
                     /locus_tag="K0W59_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
BASE COUNT          356 a          488 c          504 g          522 t
ORIGIN      
        1 cattaaaggg gttgaggaag aataaagatg ttcggaaaat tatcacttga tgcagtcccg
       61 ttccatgaac ctatcgtcat ggttacgatc gctggcatta ttttgggagg tctggcgctc
      121 gttggcctga tcacttactt cggtaagtgg acctacctgt ggaaagagtg gctgacctcc
      181 gtcgaccata aacgcctcgg tatcatgtat atcatcgtgg cgattgtgat gttgctgcgt
      241 ggttttgctg acgccattat gatgcgtagc cagcaggctc ttgcctcggc gggtgaagcg
      301 ggcttcctgc cacctcacca ctacgatcag atcttcaccg cgcacggcgt gattatgatc
      361 ttcttcgtgg cgatgccttt cgttatcggt ctgatgaacc tggtggttcc gctgcagatc
      421 ggcgcgcgtg acgttgcgtt cccgttcctc aacaacttaa gcttctggtt tactgttgtt
      481 ggcgtgattc tggttaacgt ttctctcggc gtgggcgaat ttgcgcagac cggctggctg
      541 gcctatccac cgctatcggg aatagagtac agtccgggag tcggtgttga ttactggata
      601 tggcgtctcc agctatccgg tataggtacg acgcttaccg gtatcaactt cttcgttacc
      661 attctgaaga tgcgcgcacc gggcatgacc atgttcaaga tgccagtatt tacctaggca
      721 tcactgtgca cgaacgtact gattattgct tccttcccaa ttctgacggt taccgtcgcg
      781 ttgttgaccc tggatcgcta tctgggcacc catttcttta ccaacgatat gggtggcaac
      841 atgatgatgt acatcaacct gatttgggcc tggggccacc cggaagttta catcctgatc
      901 ctgcctgttt tcggtgtgtt ctccgaaatt gcggcaacct tctcgcgtaa acgtctgttt
      961 ggttatacct cgctggtatg ggcaaccgtc tgcatcaccg tgctgtcgtt catcgtttgg
     1021 ctgcaccact tctttacgat gggggcgggc gcgaacgtaa acgccttctt tggtatcacc
     1081 acaatgatta tcgccatccc gaccggggtg aagatcttca actggctgtt caccatgtat
     1141 cagggccgca tcgtgttcca ttctgcgatg ctgtggacta tcggttttat cgtcaccttc
     1201 tcggtgggcg gtatgactgg cgtgctgctg gcagtaccgg gcgcggactt cgttctgcat
     1261 aacagcctgt tcctgattgc gcacttccat aacgtgatca tcggcggcgt ggtcttcggc
     1321 tgcttcgcag ggatgaccta ctggtggcct aaagcgttcg gtttcaaact gaacgaaacc
     1381 tggggtaaac gcgcgttctg gttctggatc atcggcttct tcgttgcctt tatgccgctg
     1441 tatgcgctgg gcttcatggg catgacccgt cgtttgagcc agcagattga cccgcagttc
     1501 cacaccatgc tgatgattgc agccagcggt gcagtactga ttgcgctggg tattctctgc
     1561 ctcgttattc agatgtacgt ttctattcgc gaccgcgacc agaaccgtga cctgactggc
     1621 gacccgtggg gtggccgtac gctggagtgg gcaacctctt ccccgcctcc gttctataac
     1681 tttgccgtag tgccgcacgt tcacgaacgt gatgcattct gggaaatgaa agagaaaggc
     1741 gaagcgtata aaaagcctga ccactatgaa gaaattcata tgccgaaaaa cagcggtgca
     1801 ggtatcgtca ttgcagcttt ctccaccatc ttcggtttcg ccatgatctg gcatatctgg
     1861 tggctggcga
//
LOCUS       DAETPZ010000251         1855 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.257, whole genome shotgun sequence.
ACCESSION   DAETPZ010000251 DAETPZ010000000
VERSION     DAETPZ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.257"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..934)
                     /locus_tag="K0W59_004330"
                     /pseudo
     CDS             complement(1..934)
                     /locus_tag="K0W59_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
     gene            complement(962..1666)
                     /locus_tag="K0W59_004331"
                     /pseudo
     CDS             complement(962..1666)
                     /locus_tag="K0W59_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
     gene            1722..>1855
                     /locus_tag="K0W59_004332"
     CDS             1722..>1855
                     /locus_tag="K0W59_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3025049.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     "
BASE COUNT          563 a          413 c          374 g          505 t
ORIGIN      
        1 gtcataagga tgttggctat aaacttgcca ataaagaagc aggcaaagct gtagaccatg
       61 aagtttgaag catcacgttc gttgatatcg cccaactcca gtgccagacg gatggtaaat
      121 gaccatactg cgacctgcat acccacataa aggaattgag ccacaatacc acgacgaaag
      181 cgcgaatttt tagccagata gcgcagcgta tccattgctg acgggcgttt atggtgactt
      241 gtctgtgcca ctttacaggt tggaaagcgg gttaaaagga acaacaccat gaccacaacc
      301 agaatcataa tcatatactt atacggttca agggtgttct ctaacatcag caccttaaag
      361 ttgtgaattt gctcggcgtt cattccggac atctgcttct caaggctttc cccctcggag
      421 aaaaccagat atttgcccaa taaaatacct gacgcagcac caatcggata aaaggtctgg
      481 ctgatattga gccgcaatgt ggcataggct tttggaccga tcattgaact gtatgtgttc
      541 gctgcagttt caaggaaact caggccaatc gcaatcgcaa aaatagctgc aaggaacata
      601 gtgtaggttg ccatatgcga ggcagggaaa aaagtgtaca accaccaata tacagcgtca
      661 ggccaattaa aattgccacc ttataactgg tctttttaat cacaagggat gctggtattg
      721 caattaaaaa ataacctcca taaaatgcgc tctgcaccaa tgctgaagca aagttactta
      781 gcgaaaatac acttttgaat tgagtgatta atatgtcatt taatgcagct gcgcatcccc
      841 atagcgggaa taaacacgat aacaaaataa actggaacaa gggagtctta ttcagatacc
      901 catcaggcat ctgaatgatg tttttatcgt tcatagtgct acctttaact gtgcaggatg
      961 attattcgtt taaggttaaa aattcattaa attgttcaat actcggataa gatgattgca
     1021 tacctttccc tgtgacgctg aaagcggcaa agagagcggc ttttttcaaa gcggcttcaa
     1081 catcaccgct ttgaacataa taatgggaaa agcaaccaat aaatgcgtca ccagcgccac
     1141 tagtatcaac agcatttact ttgaatgcag gaacatggac ttcctgatcg cgggtcatcc
     1201 ataatgcgcc tttttcgctc atggtaacaa taatattgtt cagcccttta tcaactaacg
     1261 aacgtgcggc caaacgaata tgatcataag tatcaaccga cataccggtt aatatttcca
     1321 gttctgtttc attcgggata aagaaatcac ctttgcaggc ataagacata tctaactcac
     1381 gcaatgccgg agccggattt aataacactt caataccatt tttcttacca aactcaatcg
     1441 cgtggtaaac tgtttccagt tgaacttcca gttgtaaaac gatcaatttg cattttttca
     1501 gatcttctgc agctcgatcg atatcttccg gggaaagaaa tttattcgct cccttaatta
     1561 ttaatatact attgctcgag ttggcattaa caaagatcgg tgcaacacca ctgctggtac
     1621 aggggacctt ctcaacataa gtggtattaa ttccccatga ttcaagggta atgctgccaa
     1681 cttactgatt tagtgtatga tggtgatttt aaggtgcttg cgtggcttcc atttccatca
     1741 gatgtccttc ctgctccgct actgaaggcg tggtgcgtaa cggcaaaagc actgccggac
     1801 atcagcgcta tctctgctct cattgccgta aaacatggca actacagttc actta
//
LOCUS       DAETPZ010000252         1829 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.258, whole genome shotgun sequence.
ACCESSION   DAETPZ010000252 DAETPZ010000000
VERSION     DAETPZ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.258"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..767)
                     /gene="ospC4"
                     /locus_tag="K0W59_004333"
                     /pseudo
     CDS             complement(1..767)
                     /gene="ospC4"
                     /locus_tag="K0W59_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
     gene            complement(1134..1652)
                     /locus_tag="K0W59_004334"
                     /pseudo
     CDS             complement(1134..1652)
                     /locus_tag="K0W59_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1669..1829)
                     /locus_tag="K0W59_004335"
                     /pseudo
     CDS             complement(1669..1829)
                     /locus_tag="K0W59_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          514 a          340 c          395 g          580 t
ORIGIN      
        1 caacctcttt aaagttatct aagaaaaatg attggtgctc atggtaaaaa acaggtggaa
       61 tagcattatc aaagtgctcg gttaatgtca tatatccata tggagagtca tgatcaatga
      121 tatacgcatt ggcgccaaaa tctacagtat gatgtgtttt atttagtggg agtttttctt
      181 gatgattgga aatttcaacc ccaaaaaaca aaatcgtggt tagagatttc tttaatatcc
      241 tcagagtatg tattcttatg tgtttgtata ttttcagact gaattttatc aaaggatatt
      301 aatattaaga atattatttg acagattaga gttactttga tgtctgaagt taaaattaga
      361 attaataata atagaaagta agtgacgttc gtctgaagaa agaggatgaa ctttttgtat
      421 tatctcagag atatagtgtc tggacacctt atcgagatgc tcaagtgacc gaaatgatga
      481 ccgtgctcca gatggcgctt caatagaaag atttagtgat ttgaaactgg tcccttgaga
      541 aaacattttg ctataacttt gcgctgctat agacttcctt aaaaagtttt tcacagtatt
      601 agcacagtga tctaaatgtt tttcacttat gccagaagaa gatgagtttg ttacgcgtaa
      661 tatatttttt gtaatgcttt tgttgcttta ttcggattcg tttttccatc aacaagatca
      721 atattattct gaacattaat atgatttact gcttcaggta ttttcataaa atattcccca
      781 ttatgtgcgt ttttatctat ggttatttag aatatattat ctaacttaat attttcttgt
      841 gtgtattata acgaaatgaa catattttaa tatgttgtac aatttttact ctgaaatccc
      901 ctgaaattaa tcacacctcc tttactgaaa ggaattattg caacggtatt atttgttttt
      961 ggatatatat cttgcgcctt gttatgtagt taatcctcag aatatgatag ataaaataga
     1021 gacacagagc agagcgtgtt ctaacatcat gataacataa aataattagt aacaataaat
     1081 gaaagaatca tgggagctca acaaaaagag agctaaatag ctgtgtggaa tagtagatca
     1141 ctttggcggt ataatggcag atagtctgca tactttccgg taacaacatg ggccagaagc
     1201 cccgctccgg cataactgcg tgcaatgggt tttgaaggca ccggtgcctg cacgatatgg
     1261 tcgcaccggc gacaggccag tttagggcgt tgtttttcga taaccttaaa ggcgctgctg
     1321 ataagctcca gctgctctga cacatcacat cccagtggac tgagttcacc accacaggcc
     1381 ggacagcatt cctcttccgg ccggatgacc cggggggcac ggggaagtga ggctggtaac
     1441 ggtttacggg ctgaagactg gcgcagggaa gatggcagta ccgggtcata ttgctcaccc
     1501 agtgtttccg ccatttcctc ctgaagtgcg ctgattcgct cctgtgcttc ctgtatccgc
     1561 cgttcggttt ttgcgcgaag tttttctgag cttttaccga actgcatacg ctgcagtttc
     1621 gcgaccagcg ccttcagtcg gttgatttcg gaaggcctgg acaactgaat ctgcgaccca
     1681 gacctgatta ggtgctgtgg gcgtaaagtt ctgattcagc agattcggcg taaccggcag
     1741 attgtggtca gagtttgtgg tcgccctgaa cttgcgtttc tgcttacagt gcagcctcag
     1801 ctccttacga agacgtgcca gtctgtcac
//
LOCUS       DAETPZ010000253         1828 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.259, whole genome shotgun sequence.
ACCESSION   DAETPZ010000253 DAETPZ010000000
VERSION     DAETPZ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.259"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..168
                     /locus_tag="K0W59_004336"
     CDS             <1..168
                     /locus_tag="K0W59_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBI3025050.1"
                     /translation="SAIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQR
                     MKERRKEWGIL"
     gene            153..791
                     /locus_tag="K0W59_004337"
     CDS             153..791
                     /locus_tag="K0W59_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HBI3025051.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADTGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1019..1410
                     /locus_tag="K0W59_004338"
                     /pseudo
     CDS             1019..1410
                     /locus_tag="K0W59_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1429..1800
                     /locus_tag="K0W59_004339"
                     /pseudo
     CDS             1429..1800
                     /locus_tag="K0W59_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          544 a          324 c          435 g          525 t
ORIGIN      
        1 tccgctattg ccagtacacc tcacccgtcc tggcgacgta tctgtaagac actcataaag
       61 aatgattttt ggtgccggac tctctccttc agtccgaaca aaccccggca ctatgaacgt
      121 tacctgcagc gtatgaagga aaggaggaag gaatggggga ttctgtgaca ccggagatac
      181 ttggcaacat gattcgtcag tatttcagtc aggtacgaac tgaaaaagag agcatacagg
      241 ctctgaatca tctgcgcaga gtgcttcatg aggtcagtcc ttttgcacag gagcctgtgg
      301 actgtgtgtt gtgggtgaaa gcggatgagg tggtggccaa cgattacaac cctaatgtca
      361 tggcactagg tgagaaaaaa ctactgaaac agtctctgga gaaagatggt ttcacacaac
      421 cggtggtggt gtcagaggag aagaatcatt atctggtggt ggatggtttt catcgtcagt
      481 tactggggcg ggaagcagat acaggaaaac gcctgagggg ctggttgcct gtagcctgta
      541 ttaatccgga gagaaaggga caggccgcac gtattgccgc gaccattcga cataaccggg
      601 ccagggggaa acaccagatc actttaatgt ctgatattgt cagggatctt tcacgtctgg
      661 gctggacgaa tgaacgtatt ggtacagagc tgggaatgga ccaggatgaa gtgctgaggc
      721 tgaaacagat tagcgggctg acagagttgt ttcaggaaga ggatttcagc tcggcttgga
      781 cggttcgttg atgttgttaa atatgggcgg tatttctgaa agttgatact ttgggtagaa
      841 aaaatgatac cgaaaactat ggaggatgtg cttgtcaggt gactggacac cataggtctt
      901 aggctttgat aacgggataa aatgagcggc ttttctcata ccatcaggtg tgatgatggc
      961 gcagatttac aaaatctgat tgattctaaa gatgtataag taaaaatcaa gctttgatat
     1021 gaaaaaacag atatttatta ataataaacc tcctgtagtc ccttactcag ggacgcacgc
     1081 gaaaatattt aaatacattg aaatccctct gccgtttttc tactttatct atacatcagg
     1141 cgaaccattc cacatatctg tccaaaatac tgttatttat gtatcaaaat acaatggtat
     1201 atttattaat aaattagttc ctttttctct tttatttgac agggatatat ctgttcttca
     1261 aagaagagat atttgtgttg tgcgatttac gtcagaggaa ataagtgaac ataacgtttt
     1321 atttgatcat gacattgaac gattaaaaaa atttccaaag ctcagcttat atctcccgat
     1381 tatgttttga ttgatttttc tagtgggggg ggagatgaac cctatgcaat gtcctggtta
     1441 atatctcagt gtgctcatca gtgcactgat aataaaaaga cggagacaga tgccatttac
     1501 gataaagttc ggagttctta tttgctttca tgtattctga aaaaaaacaa aaatgttggg
     1561 cttattcttc atgcaccatc atttgtttct gtgtctgaaa aaatcgccag aattgtgatg
     1621 gcaaattact cccgtaactg gagtaattcc gagttggcca gtgcggtttt aatgagtgaa
     1681 tcttctttaa aaagaagaat gtacaaagag gtcggttcta taagtacatt tgtacataaa
     1741 ataaagctta cagaagctat caggaaatta cgaaggacaa atactcccat ttcggtaatg
     1801 ctgccaactt actgatttag tgtatgat
//
LOCUS       DAETPZ010000254         1826 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.260, whole genome shotgun sequence.
ACCESSION   DAETPZ010000254 DAETPZ010000000
VERSION     DAETPZ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.260"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(39..308)
                     /locus_tag="K0W59_004340"
                     /pseudo
     CDS             complement(39..308)
                     /locus_tag="K0W59_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(423..1061)
                     /gene="ospI"
                     /locus_tag="K0W59_004341"
     CDS             complement(423..1061)
                     /gene="ospI"
                     /locus_tag="K0W59_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HBI3025052.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1220..1690)
                     /locus_tag="K0W59_004342"
                     /pseudo
     CDS             complement(1220..1690)
                     /locus_tag="K0W59_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          515 a          452 c          337 g          522 t
ORIGIN      
        1 ttaagtgata ctggttgtct ggagattcag ggagccagtc tacccagcaa gcgctgtaac
       61 tcttttattt gttttatagc ggcagccagt tctgaggcag gaacgacatc ctctccggca
      121 gcaacagcag tgagacttcc ttccagatat tattttttcc acttaagcaa caggctgggc
      181 tgaataccat gcaaacgcgc cacatgggac accgtcatgc caggctccat tgtctgctga
      241 ataatggcaa ttttttcctg aggagtttta cgtttacgaa gctccagccc cagcaaagtt
      301 ccagtcatct caaaattaga actagtgtta gacatatatc caagcctatc tcttatttta
      361 agatacatcc actgtctggc gtttcaaggg gccggctcaa atctatgtac aggctctcag
      421 catcagcaaa gcctcttact tttccaacta ctactgttag catagtgatc aaattcctga
      481 tcagatacat gaattacctg aaccgaacta ttttcatcta caaatgcatc tggcgatttt
      541 tctacaggat cataaagatt aaatcccttt cctgtttgaa catccaaaaa cataattttt
      601 tcaccatcgt taaatccagc tatccaatga gtggcctgtt caatagaaat cattactaca
      661 cttccttttg aagtattttg taaaatattt tctgcaatac gctcacgaaa tttactataa
      721 gtttcattag gatgtgacac tatgccttca ctatcaattt gctctaaatg cctatgaaaa
      781 aatccggtca tatactctga tatttcatta ggtacgggag cctctcttac tccataccct
      841 aacatagcca tacaagcatg aagcgcacac cctgaacagt taccatcccc accagacgga
      901 tttatcattt tctgtaaagt cgctgccaat tcagaatctt tatccatatc accaagactt
      961 ttagagttta aagattccag caatttttta acatttacat ttccctcctc tgtatgtgcc
     1021 tcatatccag cagttccctg taacgacacc ccattaatca tattgtatcc tgacagtata
     1081 tatactcact atattttctt cataacaaat atccctctga tatgacaaca aaaaacggag
     1141 ccggattcct gtttttgtga actgcatcta ttcgtgtcta aaaagtgatc cacttaccac
     1201 caacggccta atatatacag aacggagtaa acagggctaa cagttcccga caggtctcat
     1261 cattccgtgg cccgaaagtg taagccaaca caccgccagt tttagtgttg tacgcatacc
     1321 agagccagtg ttgttgcagc cttgctgcca acgaagcgcc actgctcatc aagttcacag
     1381 ataagggcaa catcagcgtg agcgactggc gcagatgtta tccgccgggg cgggagcttt
     1441 ttaatgtccg tatgacggta ttgatgccga ttttcagtgt cctggcggta tcacggcccc
     1501 ctgcgccgtt atgtgccatt tcaacgatct gctccttaac gccgggtttt ctggcttcat
     1561 aagagtaagt gagctggaaa acacgtttac atgagcgaca acggaaacgc tcatgccggg
     1621 aacaactacg gccatgtcga taaacttcat cggaatgaca gcgcggacaa tgaacagtaa
     1681 cggtagccat ggaaaacctt aaataccagt agattaccag aaacttctgt cgattgaaga
     1741 catcacccga agtttgaatt cattcgaata ctttttacga ggttcttttc gccagtcctg
     1801 taattcgtaa gcgccccatc agcgac
//
LOCUS       DAETPZ010000255         1791 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.261, whole genome shotgun sequence.
ACCESSION   DAETPZ010000255 DAETPZ010000000
VERSION     DAETPZ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.261"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..566)
                     /locus_tag="K0W59_004343"
                     /pseudo
     CDS             complement(1..566)
                     /locus_tag="K0W59_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(637..873)
                     /locus_tag="K0W59_004344"
                     /pseudo
     CDS             complement(637..873)
                     /locus_tag="K0W59_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(1140..1646)
                     /locus_tag="K0W59_004345"
     CDS             complement(1140..1646)
                     /locus_tag="K0W59_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3025053.1"
                     /translation="MEQAKEPIVERMAYLIRRYPSRSAAARACGININTLQSYFKKGN
                     TPEPRESILVRIAESEGVSLEWLKTGNGNPPKTQSDLSQGDQLTEMLSFLTSEEREAL
                     TNMLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVREILEEIEASILS
                     ESVNAKAG"
BASE COUNT          418 a          430 c          395 g          548 t
ORIGIN      
        1 gctgaccagc cccagctctt tcatgagcct gccagcaagc cagcgtccca tctggtagcc
       61 tctccgggtt gccattgtgg cgatgcttct tgctccggcc gaaccgtggc tgatgccatg
      121 tagctcaagt acctgactgc gtaatacagc ccgtctgccg tctggttttt caggacggtt
      181 tttccagtat ctgtagctgc tgcgatgaac cccgaacaca tggcagagtg tgaccacagg
      241 ataatgcgct ctgagtttcc cgattattga gaactgttca gggagtctga catcaagagc
      301 gcggtagcct tttttaatat ttcattctcc atttcaatgc gttgtagctt tttcctcagc
      361 ttacgtattt cgatttgttc tggtgttatc ggagaggctt ttggtgtttt gccctgacgc
      421 tcatcacgca gttgtttgac ccatcttgtc attgtggaaa ggccaacatc catagctttg
      481 gcggcatctg ccaccgtgta tttctggtca acaaccagtt gagcggattc gcgtttaaac
      541 tctgcgctaa aatttctttt tttcattgga gcacctgtgt tgttctgagg tgagcatatc
      601 acctctgttc aggtggccaa attcagtgtg ccacttcacc tgttggctgg ctgcaaccgc
      661 taatgggctg gaaccgaggt tgccgatttg cacgtcgcca gaagccagcg cccgcacgat
      721 gctggctccg ctgtcaaact tacgccagtc cacgctcgcc ccgctctctt tagcaaaggt
      781 gttgtcggcc tgagccactt tcgccggttc ggctgaggtt tgatacgcca cggtgacgtt
      841 caccgcctgt gcctgaaaag cgatgaatgc cagtgcggca agaagtgtgt tatatgacta
      901 ggtaatttat tcaagataac cgggaatggg taaaaaaaga cgggatttga tgggattcaa
      961 taagttatgc gccgattgca ttcaagataa aacgagttaa aaatgctcaa tgcttcgata
     1021 tcgataaaac attcaagata ggcgcgccgt gtattttaat tcttttaaga ctcttcaaaa
     1081 atgtcatcgt agcctttaaa aacgaatttt aaaggcaccc taaaggcagg tcaatgaagt
     1141 taacccgcct tcgcgtttac actctctgag agaatactag cttcaatctc ttccaaaatc
     1201 tctctgactt gctcgtcggg catatcaacg agcatcaacg ctgccaggcg tttacggccc
     1261 tctagttgca gtagctcttg agtttcctta tctaacaagc gtagcacagt ctcaacacct
     1321 ttccttgcga gcatgtttgt aagggcttct cgttcctcac tggtaagaaa cgacaacatt
     1381 tctgttagct gatccccttg gcttaaatca ctttgtgttt ttggtggatt cccgttaccg
     1441 gtttttagcc attcaagact aacaccttca ctttctgcaa tgcgtaccaa tatgctttct
     1501 cgaggttctg gtgtatttcc ctttttaaaa tagctttgta acgtattgat atttatgcca
     1561 caggctctgg cggcagcact tcttgatgga taccgcctaa taagataagc catgcgctcc
     1621 acgataggtt cttttgcctg ctccataaaa gtacctctag gtatttttct gtataaaggt
     1681 tcttttccat ctatctaatt gaatggaaag gtaattttaa aaatccaaat aaaatacaaa
     1741 aaaatacctt gtattggtgc tttttgtgat ctgcgatttt atttactcgg a
//
LOCUS       DAETPZ010000256         1782 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.262, whole genome shotgun sequence.
ACCESSION   DAETPZ010000256 DAETPZ010000000
VERSION     DAETPZ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.262"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..452
                     /locus_tag="K0W59_004346"
     CDS             <1..452
                     /locus_tag="K0W59_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3025054.1"
                     /translation="VGWNWNSGVYDANISGASTLILHFNKNTGSCPAVQFRVNYKNGG
                     IFYRSARDGYGFEAGWSEFYTTTRKPSARDVGAYTQAECNSRFITGIRLGGLSSVRTW
                     NGPGWSDRSGYVVTGSVNSNRDELIDTTQARPIQYCINGRWYNAGSI"
     gene            455..988
                     /locus_tag="K0W59_004347"
     CDS             455..988
                     /locus_tag="K0W59_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBI3025055.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGIVI
                     KRTYTGEEQRQQVENEKQSLLQLVRDKTQLWDSQLRLGIISAGNKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1017..1544)
                     /locus_tag="K0W59_004348"
     CDS             complement(1017..1544)
                     /locus_tag="K0W59_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBI3025056.1"
                     /translation="MMHLKNIKAGNAKTLEQYELTKNFDVIWLWTEDGKNWYGEVKNF
                     QPDTIKIVYDENNIIVAITKDASTLNPEGFSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERSVRLNMATDEECARLESWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(1546..1782)
                     /locus_tag="K0W59_004349"
                     /pseudo
     CDS             complement(1546..1782)
                     /locus_tag="K0W59_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171265.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          499 a          391 c          399 g          493 t
ORIGIN      
        1 ctgttggctg gaactggaat agcggtgttt atgatgcaaa tatcagcggt gcatcaacat
       61 taatcctcca cttcaataag aatacgggga gctgccctgc tgtacagttc cgtgtgaatt
      121 ataagaacgg cggtattttt tatcgttcag cgcgtgatgg ttatggattt gaggctggct
      181 ggtcagagtt ttacaccaca acacgcaagc catcagcgag agatgttggc gcatatacgc
      241 aggcagaatg taactcaagg tttattacag gtattcgcct tggtggtctg tcatctgttc
      301 ggacatggaa tggtcccggc tggtctgaca ggtcaggtta tgttgttacc ggttccgtta
      361 actcaaacag agatgaacta attgatacaa ctcaggcaag gccaattcag tattgcatta
      421 atgggaggtg gtataacgcg gggagtattt aacgatgatg cacttaaaaa acatttctgc
      481 tggtaatcct gaaacaaaag agcaatacca gctaacgaaa caatttaaca tcaaatggct
      541 ttatacagag gatgggaaaa actggtatga ggaacaaaag aactttcagc ctgatacgtt
      601 gaaaatggtc tatgaccaca acggcgttat tatttgtatt gaaaaggatg tttcagcaat
      661 taatccggaa ggcgcaagcg tcgttgaggt tcctgatatt acagcaaatc gccgggctga
      721 tatttcaggt aaatggatgt tcaaagatgg catagtgata aagcgaactt ataccgggga
      781 agagcagagg caacaagtgg aaaatgaaaa gcaaagcctg ttgcaacttg tcagggataa
      841 aacccagcta tgggactcac agctacgact gggcatcatt tccgccggga ataagcagaa
      901 attaaccgag tggatgctct acgcgcagaa ggtcgaatcc acagacacct ccagtctgcc
      961 agtaacgttt cccgaacaac cagaatgaga gaaggcccgc tatcgggcct taatttttac
     1021 tctggttttt gtggccattc tggctttgcc gtatccacac ggctgaccag aacactgtag
     1081 cgttcccatg actccagtcg tgcgcattcc tcatctgttg ccatattcag cctgacagag
     1141 cgctccagcg gctggatgac tgattcagct tcggaaagca atgcggcttt ttgtgattcg
     1201 gcctgttgct gctgttcgtc tgccgtataa atccgcttaa ccacagctcc gtccttaaac
     1261 atccacttcc cggaatcatc ggcacgacga tttgctgtta tatcggggac ctcaacgacg
     1321 ctaaagcctt caggattaag cgtggaggca tctttagtga tggcgacaat aatattattt
     1381 tcatcgtaaa caatctttat tgtatctggc tgaaagttct tcacttcccc ataccagttt
     1441 tttccgtctt ctgtccacaa ccagattacg tcaaaattct ttgttaattc gtactgttcc
     1501 agtgttttag cgttacccgc tttaatgttc tttaagtgca tcatattaaa cgctcgctac
     1561 attataccaa gtgccattta tatacttttg aatcggtctg taataaacac ccgcaatatt
     1621 atcggcaaag ttgaacctgt atcctgaaca ttaataccag acaatacatg acctgaaggg
     1681 cactgaaaat tccatgtttg ccagttgttc actccataat attgctgtga accaagtcga
     1741 acatctttca catatctgga atcaaaattg ccatagttgc cg
//
LOCUS       DAETPZ010000257         1774 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.263, whole genome shotgun sequence.
ACCESSION   DAETPZ010000257 DAETPZ010000000
VERSION     DAETPZ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.263"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..284
                     /locus_tag="K0W59_004350"
     CDS             <1..284
                     /locus_tag="K0W59_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3025057.1"
                     /translation="KSDYLPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAG
                     IYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            286..890
                     /locus_tag="K0W59_004351"
                     /pseudo
     CDS             286..890
                     /locus_tag="K0W59_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
     gene            877..>1774
                     /locus_tag="K0W59_004352"
     CDS             877..>1774
                     /locus_tag="K0W59_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3025058.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLPDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRVF"
BASE COUNT          426 a          404 c          496 g          448 t
ORIGIN      
        1 aaaaaagtga ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg
       61 catttaatgg tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc
      121 gctactatgc cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg
      181 tgagccgtga cggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg
      241 ttctggatgt gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct
      301 gccactgtgc ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt
      361 aacgcagcgc tttccccgac agttttatca gtgattttta tggtcttatc tggaacatcg
      421 ataccgcaga aaagtatggt cttgatgtct ggggaaagat tgtgggtgtc agtcgccggc
      481 tgacggtaaa ggacgatttt aattacctgg gcttcagcga gtccaggatg gacaccccgg
      541 taatggatga tccctgtccg tttaatcagg caccgtttta caacggaaaa tcggataccc
      601 ggactgttga cctgtctgat gctgtatacc ggcggctgat actgatgaaa gccatgtcga
      661 acattactga ctgttccgtt ccggatatta acctgatgct gagatttatg ttcggaaaaa
      721 aacgccgggc ttatgttctg aataatggcg ggctgaggat gagttacgtc tttgagtccg
      781 cgctctcgtt ggcagaactg gcgattatcc agtcgtcggg tgcactgcca tccccgccgg
      841 gtgtttatgt ttcagtagtt ttaaaggagt cccgtaatga aggccagtga taaaccccgc
      901 cagctggcgg tcccctttgc gagtaccgga gataaaaacc gtatcccgga caaggcgaca
      961 cagcagacca gagagagcgg taatgctgcg tatgattcag gttttcctcc ggtgaccatg
     1021 acagcggtct cagcgggagg tataccgcca cacggcaagg attttaacgg tctgatgtac
     1081 gatattaccg cagcaatacg gttcgcccag gctggcggtt tgtacacgta taatgccggt
     1141 tttgcggggg ccattggtgg atatgcaaaa ggagccattc tcgccggagt cgcaacaaca
     1201 gcggtctggc tgaataccac ggacgataac ctgaccgatc ctgaaggctc cgacagtgcg
     1261 ggctgggtaa atcttcttga ggatccgaaa aggatattcc tgcggcagaa gaacaatctg
     1321 ccagaccttc agaataaagg gacggcacgg gataatcttc aggtttacag taaagagcag
     1381 tcagatcaac gctatgttca tcgggaaggc gataaaataa ccggagagct gaaaatccgt
     1441 ggtgttaatg cgctgaggat tttcaacgaa gcttttggcc tgatttttcg tcgttcggaa
     1501 gagtgcctgc accttattcc caccagtgaa ggtcaggggg aaaatggcga tattggtccc
     1561 ctgcgcccgt tcaccattaa tttgcggacg ggtgaaatat ccatgtcgca taaagtgtct
     1621 gttggcggtg gttcgcaggt caatggtgcg ctgggtatcg gcgttcagaa cgccctgggg
     1681 gggaattcaa ttgttcttgg tgataatgac accggattta aacagaatgg agacggtatt
     1741 ctggatgttt atgctaatag tcagcgtgta ttcc
//
LOCUS       DAETPZ010000258         1764 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.264, whole genome shotgun sequence.
ACCESSION   DAETPZ010000258 DAETPZ010000000
VERSION     DAETPZ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.264"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(417..608)
                     /locus_tag="K0W59_004353"
     CDS             complement(417..608)
                     /locus_tag="K0W59_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBI3025059.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            673..996
                     /locus_tag="K0W59_004354"
     CDS             673..996
                     /locus_tag="K0W59_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBI3025060.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            986..>1764
                     /locus_tag="K0W59_004355"
     CDS             986..>1764
                     /locus_tag="K0W59_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBI3025061.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAEKELTPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGR
                     LELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLP
                     DNLPETKQALAESVTRGIDALYHVGEIKGRQNVIQALTEAGIDVVRVTRSSISIADPN
                     GGKNVRLKGAFYEQSFTDGCGVREKAERESRIYRENAERRV"
BASE COUNT          399 a          447 c          543 g          375 t
ORIGIN      
        1 aacgccagcg gcgcagcctt tctttgccct ttgccttttt gctcacatgt tctttccggc
       61 cttatcccct gattctgtgg atcaccgtat taccgtcttt gagtgcgctg ataccgctcg
      121 ccgcagccga acgaccgagc gtagcgagtc agtgagcgag gaagcggaag agcgccggaa
      181 gctgcatttt ctccttacgc atctgtgcgg catttcacat cggctatggt gcacttttca
      241 tacaatccgc actgatgccg cataagtaag cctgtgtaca ctccgctatc gctacgtgac
      301 tgggtcatgg ctgcgccccg acacccgcta aaacctgctg acgcgccctg acgggcttgt
      361 ctgctcccgg catccgctca cagacaagct gtgaccgtct ccgggagctg catatgtcag
      421 aggttttcac cgtcatcccc gaaacgtgcg aggcagctgc ggtaaagctc atcggcgtgg
      481 tcgtgaagcg attcacagat atcagcctgt tcatccgcgt ccagttcgtt gagcttctcc
      541 agcagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt
      601 ttagtcactg atgcctccgt gtaaggggga tttctgtttt atggggtaat gataccgatg
      661 aatcgcgaga ggatgctcac catacgggtt actgatgatg aacatgcccg gttactggaa
      721 cgctgtgagg gtaaacagct ggcggtatgg atgcggcggg tctgcctggg tgagccggtt
      781 gcccgttccg ggaaactgcc gacactggca ccgccgttac tgcgtcagct ggctgccatc
      841 gggaataacc tgaatcagac agcgaggaag gtgaacagcg ggcagtggtc ttccggtaac
      901 cgggttcagg tggtggccgc actgatggcc atcggggacg agctgcgccg gctgcgtctg
      961 gccgtcaggg aacaggggac gcgggatgat agttaagttt catgcccggg gaaaaggtgg
     1021 tggcagtggt ccggttgatt acctgctggg aagggagcgt aaccgggaag gggcaacggt
     1081 gcttcagggt aatccggaag aagtccggga actcatcgat gccacgccat ttgcgaagaa
     1141 atacacgtcc ggtgttctgt cgttcgcgga gaaggagctg acgccgggag gacgtgaaaa
     1201 agtgatggcg agctttgagc gtgtactgat gcccggtctc gaaaaaaatc agtacagcat
     1261 cctgtgggtg gagcaccagg acaagggacg gcttgagctg aattttgtca ttccgaacat
     1321 ggagctacag accggaaaac gcctccagcc gtactacgac cgcgcagaca ggcctagaat
     1381 tgatgcctgg cagacgctgg ttaatcacca ttacgggctg catgacccga acgccccgga
     1441 gaaccgcagg acgctgacac tccctgataa tctgccggaa acgaaacagg cgcttgcaga
     1501 gagcgtcaca cgcgggatag acgctcttta tcatgtcggg gagataaaag gccgtcagaa
     1561 tgtgattcag gcgctcacag aggcagggat tgacgtggtc agggtgacgc gaagcagtat
     1621 cagcattgca gacccgaacg gcgggaagaa tgtcagactg aaaggagcgt tttatgagca
     1681 atcttttaca gacgggtgcg gagttcgaga aaaagctgaa agagagagcc gaatctacag
     1741 agaaaatgct gaacgacgag ttca
//
LOCUS       DAETPZ010000259         1746 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.265, whole genome shotgun sequence.
ACCESSION   DAETPZ010000259 DAETPZ010000000
VERSION     DAETPZ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.265"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            44..976
                     /locus_tag="K0W59_004356"
     CDS             44..976
                     /locus_tag="K0W59_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBI3025062.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1052..1126
                     /locus_tag="K0W59_004357"
     tRNA            1052..1126
                     /locus_tag="K0W59_004357"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1085..1087,aa:Arg,seq:cct)
     gene            1288..>1746
                     /gene="intS"
                     /locus_tag="K0W59_004358"
     CDS             1288..>1746
                     /gene="intS"
                     /locus_tag="K0W59_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HBI3025063.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
BASE COUNT          492 a          398 c          438 g          418 t
ORIGIN      
        1 ccgctacgct ttagctatac gtgctaattc aagagaagag accatggaca acgacaaaat
       61 tgatcaacac agcgacgaaa ttgaagttga gagcgaagaa aaagagcgcg gcaaaaaaat
      121 agaaatagat gaagaccgac tcccctcccg agcgatggca attcatgagc atatccgcca
      181 ggatggtgaa aaagagctag aacgcgacgc aatggcgcta ctgtggtcag ccattgcggc
      241 gggtctgtcg atgggcgctt cgttactggc aaaagggata tttcatgtcg aactggaagg
      301 tgtgccgggc agcttcttgc tggagaatct cggttatacc tttggtttta ttatcgtcat
      361 aatggcccgc cagcaattat ttaccgaaaa caccgtgact gcggtactac ccgtcatgca
      421 aaaaccgaca atgagcaacg ttggcttact tatgcggtta tggggagtcg tgctgctggg
      481 taatattctc gggactggta ttgctgcgtg ggcatttgaa tatatgccta tcttcaatga
      541 agaaactcgc gatgcatttg tcaaaatcgg catggatgtg atgaagaaca cccccagcga
      601 gatgtttgcc aacgcgatca tttccggctg gctgatcgcc actatggtat ggatgtttcc
      661 tgcagcaggt gcggcaaaga ttgtggtgat tatattgatg acctggctta ttgccctggg
      721 tgacaccacc catatcgtgg tcggttctgt tgaaatcctc tatctggtgt ttaacggtac
      781 gctgcactgg agcgatttca tctggccctt cgcactacct actttagcgg ggaacatctg
      841 cggcggcacc tttatcttcg cgttaatgag tcatgcacag attcgtaacg acatgagcaa
      901 caagcgtaaa gcagaagcac gccaaaaagc agaacgtgcg gaaaacatta agaaaaatga
      961 taaaaaccca gcataaatgg cgagggttta agcaatcgag cggcagcgta cttaccccgc
     1021 agtccattag cgggtatact catgccgcat tgtcctctta gttaaatgga tataacgagc
     1081 ccctcctaag ggctaattgc aggttcgatt cctgcagggg acaccattta tcagttcgct
     1141 cccatccgta ccagtccgca aaatcccctg aatatcaagc cttccgtata ttcacagttc
     1201 gtcatggttc gcgtcagatc gttgacagcc gcactccatg acgggtaaaa agtggataaa
     1261 ataattttac ccaccggatt tttacccatg ctcaccgtta agcagattga agcagcaaag
     1321 ccgaaagaaa aaccataccg ccttctcgat ggtaatggcc tgtaccttta tgtccctgta
     1381 tccgggaaaa aggtatggca gcttcgctac aagattgacg gtaaggagaa aatcctgacc
     1441 gtcggaaaat atccgcttat gactttgcag gaggcaaggg ataaagcatg gactgcgagg
     1501 aaagacatct cggctggcat cgatccggta aaggcgaaaa aggcttcgtc taacaacaat
     1561 tcatttagtg cgatttacaa ggaatggtac gaacacaaga agcaagtctg gtcagtaggg
     1621 tatgcgactg aacttgcaaa aatgtttgat gacgacattt tacccatcat cggcggcctt
     1681 gagattcagg atattgagcc gatgcaactg ctggaagtaa tccgcagatt tgaagatcgc
     1741 ggtgca
//
LOCUS       DAETPZ010000260         1735 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.266, whole genome shotgun sequence.
ACCESSION   DAETPZ010000260 DAETPZ010000000
VERSION     DAETPZ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.266"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(219..773)
                     /locus_tag="K0W59_004359"
     CDS             complement(219..773)
                     /locus_tag="K0W59_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HBI3025064.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(770..1060)
                     /locus_tag="K0W59_004360"
     CDS             complement(770..1060)
                     /locus_tag="K0W59_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBI3025065.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1060..1659)
                     /locus_tag="K0W59_004361"
     CDS             complement(1060..1659)
                     /locus_tag="K0W59_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBI3025066.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          417 a          478 c          394 g          446 t
ORIGIN      
        1 ggtggcgaac tgtgcagggt tggcgtaacc gggtacggaa accggcgagc ctttcggctc
       61 ccccacacag cccgccataa atcgcgaatg tgactgtgca aacgatatga aaaaagacgc
      121 gggcgcgtct catatcgctc cgtaaacatc cgggacgcca atcccgacgc cagattttgc
      181 tggcgcgtga ggaatatagc cccggataac agattgagtc aacaaacggt ttttagatcc
      241 ccggaagaga atgcatcacg catcggtaga tagagcatat attcagcaag atttagccat
      301 acatctaccc gactgcagca cgtccggaaa caccactcag ggtgtgcgtc attcagcaat
      361 tcagccattt tacgcttagt catcccccgc ccttcatacc gttgccggag gacactaatc
      421 aatccaggat gctctgccag cacctcactt atgactctat caatacataa cgcctctgca
      481 tcagtacaat gcgccagcca ggtcttttgc ttgccattga tcatctctcg caaaaacgct
      541 tccagctcag gtttctctat tcccgctttt ttcatcctgc gcagggcttc attgacggct
      601 gttttcgtca attttttgga tgccagcaac tggttaaaca tatttcccgt cttaccgccg
      661 ccaatatacg accagcgccc ccacatgcgc agttttccct gaatccagac actttccagc
      721 gtggtgagac gaaggtgttc cccgcttttg cctgtatttg ttgggtaaat cataaataac
      781 cttcctttct ccagatttct tgtgtgcgaa aaacaccttc tgcatgcatc aggcgtaatt
      841 cttctttggt gtaatcgctg gtttttaccc gcccgtcgat taaatcgtgg catgagctac
      901 aggcaatcgc tgcctgcata tcgtgtggtt ttgtcgctgt tccgcacgtc cccgccagcc
      961 tgtaatgcgc cagcacagaa gtttcgggat tgtgattgca gtagccagga attctgacgg
     1021 tgcacatctg cccccgcgcc gctttacgta aatccaccat tacgcaaact ccagtaactg
     1081 cgcggccaca ttttcgactt cctccggaga ggaaaattta cggaacagga tccagttcca
     1141 cagcacattc agtacagatt tataaacctg ctgaaactcg gtttcgtcca tgttcgcaaa
     1201 tgcgatagat tttgccctgc gcccacgact accatcagga taaatatgct cggtgtaaaa
     1261 tccggcctga atggttaccc actcgcggaa agcgtcaaac gactttagca atgccgtatc
     1321 ccgggttctg catgtcgcaa ctgtattaag gtattgctct gcggcatcac tcagggctgg
     1381 agtgtgttcc cgacctactg attcgcacag gtaatcaacg aagcctgata ccagttttcg
     1441 ttcgcgaggc gtgatcgccc caccgaccgg agtccagtaa tcaaatccca gttgcaggag
     1501 tttgaaaaaa cgcttgtgga atgcgtagtt acgcacacgc ttaaagtctg cgtgtatcca
     1561 ctcacctatt ttgatttgat gcaaaaaatc gcaactctcc ggcgtcgccg ggagaagtaa
     1621 tccggaagag gtttgtttga ccagttgtat atgcgccatc gtagttctcc gctggcgcag
     1681 tagaatgggt gttcagcccg ttatgtagta taccagaatt aatgccaata ctaac
//
LOCUS       DAETPZ010000261         1726 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.267, whole genome shotgun sequence.
ACCESSION   DAETPZ010000261 DAETPZ010000000
VERSION     DAETPZ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.267"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..546)
                     /locus_tag="K0W59_004362"
     CDS             complement(<1..546)
                     /locus_tag="K0W59_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3025067.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDGI"
     gene            complement(1208..>1726)
                     /locus_tag="K0W59_004363"
     CDS             complement(1208..>1726)
                     /locus_tag="K0W59_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3025068.1"
                     /translation="SKMVDVLESSKLLKTVELPVLTGSLTAKADTIMDALYENTESSF
                     GSEVTDYGIIAHESHLKALSRLAAKQGFGGEDAIVDMLGTDIAYYNGADRGVFMMAKR
                     FTALSFGCFRHDGENITVVLSRDGNTQSHDLEILGKVFVVAEAATTVKMGTGSAAAVL
                     PVVKRLSFTKTS"
BASE COUNT          493 a          385 c          388 g          460 t
ORIGIN      
        1 aattccatcc agtcgttttg ggttgctaat gatgtcctgc atccgcttaa tctggtcggg
       61 cgtagacata taaccccaca gggtgagcag aacctttttt gcggctacac tatttccttg
      121 gtctgatact aatgccatat agccccataa actatccctc agccagacag catcatagtg
      181 ggtttctgcc gctgttttgt catcttcgaa agtagaagca agaataaacg ttccccaggg
      241 tttctccgtc aacgttaact ttcgtgtgtt ttctgttaat gttagcaatc cttgaatttc
      301 gtctttggta tagtgactct ttatttcatt ttttaatttg gctgagtgaa tcagaagacg
      361 ataattagga tacttatctg gatttaacga taaataactt ttatctgttg ctgcccccct
      421 ggaattaaga atgttaaata cagattcgct tgtcacttct gcctctgtcg cctggcatgt
      481 gccgcacgaa aatataccag cgagcagaag agttaatgca gatttccctt tcataaaata
      541 atccatcaat cgtttgttaa aagcgtagat aaccacttat tcaacaactt gaaaataagg
      601 tattttacct gggttgttac aaaaggattg cattgcgtaa acgcttttta tttacaaaaa
      661 aatggggaag tgttacagcg attgcgaaaa gcctgaagtg attgaattaa gataatatag
      721 aaaatcagat acagcaaaaa tggcagatta atcaggtgaa gataaattgg agcgggcgaa
      781 gggagccgag gacaactagc taacttcttg tttcttaaag actgatgaat ccagtggtta
      841 cagtgatggt tacacatgga gaggctaaag gtcaagactt gaagtacgta acattcccac
      901 aaaaggaata gaacacagtc acggactacc cggccctttg gtctgactga gggtcgggct
      961 acggggtaaa tcgtcaggct tattgtatta gatggtctct cacatttttc agtcaaaaac
     1021 tcatcgtact ttaggccaga ccatcagcat gactatcaga gccagcttta gcaggaagac
     1081 cacgagatag actttgtgta ggtccattag gtcctcctct ttccgcaccc acgaactaga
     1141 ttactagtac aaaataaagg ccagacatcc agttacggaa gtctgaccaa taagtcgtta
     1201 ttgttagtta gcttgtctta gtaaagctca ggcgtttgac tacaggcagg acagcagcag
     1261 cgctgccagt ccccatctta acagtcgttg cagcctcagc gaccacgaag accttaccga
     1321 gaatctcaag gtcgtgagac tgggtgttgc catctcggga taagaccaca gtaatattct
     1381 cgccatcgtg acggaagcaa ccgaagctca gagccgtaaa gcgcttagcc atcatgaaga
     1441 cgccacggtc tgcaccgttg tagtaggcga tgtctgtacc cagcatatcg acaatagcgt
     1501 cttcgccacc gaagccctgc ttagcagcca gacgggaaag agccttgagg tgtgactcat
     1561 gggcgatgat accgtaatca gtaacctcag aaccgaaaga agactcggtg ttctcataca
     1621 gagcgtccat gatagtgtct gccttagcgg tcaggctacc agtcaggacc ggaagctcta
     1681 cggtcttcag cagcttagag gactccagaa cgtcaaccat cttaga
//
LOCUS       DAETPZ010000262         1680 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.268, whole genome shotgun sequence.
ACCESSION   DAETPZ010000262 DAETPZ010000000
VERSION     DAETPZ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.268"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(72..539)
                     /locus_tag="K0W59_004364"
     CDS             complement(72..539)
                     /locus_tag="K0W59_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBI3025069.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFFALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(605..844)
                     /locus_tag="K0W59_004365"
     CDS             complement(605..844)
                     /locus_tag="K0W59_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HBI3025070.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(979..1584)
                     /locus_tag="K0W59_004366"
     CDS             complement(979..1584)
                     /locus_tag="K0W59_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI3025071.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          544 a          369 c          294 g          473 t
ORIGIN      
        1 agacctactg ctatcacaat tactgagaga aaaaaaagca tccgatgatt catttatgct
       61 gtcctaaaaa attaacaaaa caatgggaaa acaggatatg taagcagaaa agccaatgaa
      121 attggtacag cataaggcac accgttttca cgaattgatt ttcgaaaaaa aagaagacct
      181 gccagaacaa ccagagcgcc gcaaaaggtc attgcgacga gaaaattagc cagttcatgt
      241 tcatcaatag ctaacgaaag aacagttaac agcttgacat caccgccgcc aattatccct
      301 gcggaaaaaa gaaagaagcc cacgagcaaa acgctcccgg ctgcaaccag attgggtttt
      361 tgatgctgta aaatgagtgc aaaaaaaata agcacggtta acaccagtaa ataacgatgg
      421 ctaataattc tgagtaatat atctccccat gaaataaaaa taagaaagag cgcaacaaat
      481 aaaagatcga ctcttagtaa ccatattgct gccattgaaa gactccatgt accatacaag
      541 gttgctgcca gacatattcc tttacgtctg acagcctcta gagagttttt ttatttttta
      601 taagttacgg attactcgtt ccggaaatcg ttacctgttg aattgctgca gcgatagcgt
      661 cgaatgcatc tttaattgcg cccaggaagc ctgagttctg atcgccaaag atgaaagcaa
      721 gcagagttgc catagcaaca ccaatcaatg catattcgat tgcagtaaca ccacgttcat
      781 ccttcgcgaa acgcgcaaag cactgtttag ttttaccata gtacttagta ataaaagtat
      841 tcatgttttc ctcgtatatt attatttaaa ttcaaaccac cagcttcatc tgcgtgtcac
      901 cggtatccca tatttttggg atgtggctat aattatccat tcccgctatt tagtcttcaa
      961 aaaaaattac atttaatatc atttatcttg attaagaaat agtaagacta aaaaaacaag
     1021 cgtcgtcagc cagcacactg ctaacacggt aagaacgcta ataaaaacac tccactgctt
     1081 cgttctatac cattcaatat ttttgtcagt atgttttatt gggagcagct cttcagtaga
     1141 gaattcctgg ctgttatcat tctcaggaat gtttgcatca gtgtttactt ccctggcctc
     1201 gctatcgttg gtatagctac tactgtcttc atcactgtca cgctcattct cacagacatg
     1261 tttctcgacc tttcccagat atctgacgcc ttttcgcgga atggttttaa ttagagaact
     1321 cgacaaacct gcgacgtcta atgcctttct aagcatgtaa atctgcccat aataactgct
     1381 gtcgcttacc gaacctttct gctcccgcca aacctcattg ataatatgtt gcttatcgga
     1441 ttctccctgt agtaaaagga gtaaaaatcg atagccgttt tcagacagat tgattacatt
     1501 gcctgtactg gtggtgatac gtcgagacgc tgggtaaaac ttaactccct gccctaaaga
     1561 aaaataaaaa cggtcatcgc tcataagtac cgaaccctgc tttcttcttc acatgactac
     1621 tcattacaac tttgtatgaa gtaagatgca aaatcatatt gtttacatta agttaagcaa
//
LOCUS       DAETPZ010000263         1606 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.269, whole genome shotgun sequence.
ACCESSION   DAETPZ010000263 DAETPZ010000000
VERSION     DAETPZ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.269"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..>1606)
                     /locus_tag="K0W59_004367"
     CDS             complement(<1..>1606)
                     /locus_tag="K0W59_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBI3025072.1"
                     /translation="VIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQRFTQPY
                     ASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQAAENYGS
                     AALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSLRLVGYR
                     YSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLSRQQYWH
                     TDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLSLSVPLS
                     GWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVGGNMSLT
                     YDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAPGAAGLE
                     IDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPTRGAVVK
                     AEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSGLPEKGQ
                     VTLSWGENASTKCIFNYSLSTPESESG"
BASE COUNT          413 a          416 c          346 g          431 t
ORIGIN      
        1 atccgctctc actttctggt gtcgaaagtg aataattgaa gatgcatttt gttgaggcgt
       61 tttctcccca ggataacgta acctgtcctt tttctggtag accagaaaga taaagttcgc
      121 cacggtcacc gacaattcct gaatctgcca aacttgcatc ttgtatcgca gcaatggctc
      181 caaaaggtac cggtttacca tttgcattta acactctgaa gagaacgcga taacccacat
      241 gagtcacaaa ctccgcttta accactgcac cacgcgtggg aaccatcgta acgacggtat
      301 tatcaagttc tatattcgac ttcgagaaat agttatcgct gattgctacc cgattttcat
      361 cataagggtt tagctgtgtc ttgaccgtat agccgcgcca gtccgtcgca gcaccgcgca
      421 tattatctat ttctaacccg gcggccccag gtgctttaac aagagcaata gtttcaccta
      481 actcctgact taacgttacc ccttcgctgt gtgccagaat tccccctctg ataccataat
      541 tcagcctctg ggaattttgg ctgtagttat agctgccgtt caccgtgcca taagagccat
      601 cataggtcag ggacatatta ccgccaacct catcatcatg actgttatag gactgcatta
      661 aattccatga aaggttattt ctttccagcg ccgtaccgct gagtccgagt tgatgtgagc
      721 taccggaatt atcgttttgc gaaacagaat aggtggcgta actattaccc caccagcccg
      781 ataaaggaac agataaagag agcatgtaga tctgctcact ggtgttatca ctggcatggt
      841 tgttccccgt cccctgtctg gcagtattgg aataactcca ggagacgttc cacgagagac
      901 gcttccagct actgctgtag ccaaattgca ttaatcgttc gacatcatcg gtatgccagt
      961 attgttgccg gcttaatgtc aggtataaat tgccataatc tctccccaac gactgcgtta
     1021 gcgttccctc cacgcgacta cgtcggttac ctgtttccca aaagtcttca gggctgttgc
     1081 gccgcgatgc ccactcattg agggtatagt agccctcggt ggagtaacga tagccaacca
     1141 ggcgcaagct agtgtctgtg tcgtcaaata gttttgaata gagaaagcga tatgactgac
     1201 ccgtttctgt atcatcatgg ctaaaattcg cacgagcatg tgtcacatcg aaagaaattg
     1261 cgcccaaagc gccaagatct ttaccgacac ccagagctgc agaaccataa ttttcagcag
     1321 cctgcatacc tccatataaa gtgatcccgt gggaaaaacc atgaagtatt tgcgcctgaa
     1381 ggacgtccgg tgtaaatccg tcttcatcgc gcaattcacc cacgctgaca tcaacatctg
     1441 tcagaccttc acgtttaaga atcgccaatg aagcataagg ttgggtgaat cgttgttcgc
     1501 taccgtcttc ttctttgatg gttacgtcca aatcgccacc tgtacttgcg gtgttgagat
     1561 cggtgatctc aaaagcacct tgtggaacgg ctgactgata aatcac
//
LOCUS       DAETPZ010000264         1520 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.270, whole genome shotgun sequence.
ACCESSION   DAETPZ010000264 DAETPZ010000000
VERSION     DAETPZ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.270"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(248..322)
                     /locus_tag="K0W59_004368"
     tRNA            complement(248..322)
                     /locus_tag="K0W59_004368"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Gly,seq:tcc)
     gene            complement(326..401)
                     /locus_tag="K0W59_004369"
     tRNA            complement(326..401)
                     /locus_tag="K0W59_004369"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(366..368),aa:Thr,seq:tgt)
     gene            complement(407..480)
                     /locus_tag="K0W59_004370"
     tRNA            complement(407..480)
                     /locus_tag="K0W59_004370"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(444..446),aa:Arg,seq:tct)
     gene            complement(482..557)
                     /locus_tag="K0W59_004371"
     tRNA            complement(482..557)
                     /locus_tag="K0W59_004371"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(522..524),aa:Ile,seq:cat)
     gene            complement(734..1423)
                     /locus_tag="K0W59_004372"
     CDS             complement(734..1423)
                     /locus_tag="K0W59_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HBI3025073.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQGNAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRLEQKA
                     AFSKQSIAKVD"
BASE COUNT          395 a          408 c          322 g          395 t
ORIGIN      
        1 cccggggttt gcttagaata ttttgtaagt tgtccgaaga gatatttaca acaccagaat
       61 gatgaatcat cagtcccctg ccagaggttc actacgtaac ctgaccgaca atgcaccatt
      121 ctggtgctga aaaaagagca ctggaactgc aacaaaacaa catcacagaa caggagatgg
      181 taaggagtca gacattccca cacaatattg tgtcagtgca aataacaacc ttcgtctcaa
      241 atctcgctgg agcgggcagc gggaatcgaa cccgcatcat cagcctggaa ggctgaggta
      301 atagccatta tacgatgccc gcatatggtg ccgactaccg gaatcgaact ggtgacctac
      361 tgattacaag tcagttgctc tgcctgctga gctaagtcgg cgcaggccca ccaccgagga
      421 cttgaacctc ggaccgtcaa cttagaaggt tgatgctcta tccggatgag ctagtggtgg
      481 ttggtggccc ttgctggact tgaaccagcg acctggcgat tatgagtcgc tcgctctcac
      541 cactgagcta aagggccgga ggcagaataa taaccatatg tcatcacatc tgcaaactca
      601 tctgaccacc agcacgttta acgtcctgtg ccatttttca ggcataaaaa aaccgctcgg
      661 cggcgggttt tgcttacttt accatcgcgt acaaaatcgg caaagtatca gatttgcatg
      721 aaatatatgc cttttaatct acttttgcaa tactttgctt tgaaaatgcc gctttttgtt
      781 ctaaacgtgt tctcatcaca aacaataaag cctcactatc cagtcggtga aaaatgtgtt
      841 tcattacaac ccagtgacca gtaaatgttt tggaccagtt tttggttgtt actcccgcca
      901 gtaatgccag ctcctgatat tcataacctt ccccaccaaa aagttctgct tttaccgcct
      961 gcgccgccag ccagattaat tttttcaggc gttcctgtgt tttctctgca attttcctag
     1021 tgccagaatg agccttaaac tcttcccacg cccagtgggt tatggtgatc tgatactccc
     1081 agcaaatact tccgctgtaa caccacaaca accaggcttt atggtgctct tcaagagaca
     1141 gaacagcccg ccgccacgat gatgtcgaaa acgcaacctg actgaccaga ggaattgacg
     1201 cccccttcgc cagtgattgt tttcccggga ttggtggatt atcccgcgtt atcatttttc
     1261 cggtcacctc atcgcggtac cggatttttt tacgcctgta acgccctgta tcgaacatgg
     1321 cattcccctg ccaggcttca agctgacctt ttgtcgcccc actcaaatca gcggtggcga
     1381 taatgagctg ctcacgaaca aactgtaaat actggttatt catgcacact ccagctctgt
     1441 gatttttatc cccaaccgac caccaggaac aggcagtccg cgcacaatat tggtttcatc
     1501 aaactgctcg tcgtcgataa
//
LOCUS       DAETPZ010000265         1516 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.271, whole genome shotgun sequence.
ACCESSION   DAETPZ010000265 DAETPZ010000000
VERSION     DAETPZ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.271"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..144
                     /locus_tag="K0W59_004373"
     CDS             <1..144
                     /locus_tag="K0W59_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3025074.1"
                     /translation="GLSGDADAEREAGAQVMRETEQQIYRQLTDEVLALRLSENGSQL
                     HHS"
     gene            352..621
                     /locus_tag="K0W59_004374"
     CDS             352..621
                     /locus_tag="K0W59_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3025075.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            618..844
                     /locus_tag="K0W59_004375"
                     /pseudo
     CDS             618..844
                     /locus_tag="K0W59_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            873..1463
                     /gene="ospG"
                     /locus_tag="K0W59_004376"
     CDS             873..1463
                     /gene="ospG"
                     /locus_tag="K0W59_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HBI3025076.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          432 a          301 c          381 g          402 t
ORIGIN      
        1 ggtctgagcg gtgatgcgga tgcggagagg gaagccggtg cacaggtgat gcgtgagact
       61 gaacagcaga tttaccgtca gctgactgac gaggtactgg ccctgcgatt gtctgaaaac
      121 ggctcacaac tgcaccattc ataatcacat cgcataaaac tgtcagcgcc agtgatagga
      181 caggtgaaac ctgtcaatac gtaccggctt acgccgggct tcggcggttt tactcctctg
      241 ccatatgcaa caacggagtg ccgccttaca tgccgctggc gcggcatatc ctggtgacga
      301 tatctgaaca gttatatact gtgtatatac gtggtaatga cgaggtggca aatggcacag
      361 gttaatatga gtgtaagaat cgacgcagaa ctgaaggatg cttttatggc cgctgcaaaa
      421 agcatggacc gtaatggctc tcagttaatc cgggatttta tgcgccagac cgttgaacgg
      481 cagcacaatt cttggttccg tgaccaggtt gcggcaggcc gtcagcaact cgaatgcggt
      541 gatgtgcttc cccatgacat ggtcgaatcc tctgctgctg catggcggga tgaaatgagc
      601 aggaagattg ctgataaatg atggagatgt tctggaccat gctggccatc caggacagga
      661 agcgcattcg tagtacattg cggagcagaa cctgatggcc gccattgaac cggacgaacg
      721 gattggttat tcagccagca gcctggcagg gcagccgtat aaaggccgca acggtcgtgt
      781 ggaaggaaca tctggaccac acaaggtagc gtgtaacgtt attttgtgtg aaaatctctt
      841 gtgattttta aatatcgtaa caaaggtatg acatgaaaat aacatctacc attattcaaa
      901 caccttttcc atttgagaat aataattctc atgctggcat agtaacggag cccattctcg
      961 gtaagttaat aggtcagggg tcgacagcag aaatctttga agatgtgaat gattcatctg
     1021 ctttgtataa aaagtatgat cttattggca accagtacaa tgagattctg gaaatggctt
     1081 ggcaagaatc tgagcttttt aatgcttttt atggcgatga agcatccgtt gttatacagt
     1141 atggcggaga tgtgtacctc cgaatgctgc gcgtgcctgg gactcccctt agtgacattg
     1201 atacagctga tatccctgat aatatagaga gcctttatct acagttgata tgtaaattga
     1261 atgagttgag tataatccat tacgatctta atacaggtaa tatgctgtat gataaagaaa
     1321 gtgaaagttt attcccaata gattttcgca atatttatgc tgaatattac gctgcaacca
     1381 aaaaagataa agagattatc gaccgacgat tacaaatgcg tacaaatgat ttttattcgt
     1441 tattaaacag gaaatattta tagccgtatt tgttgatgct ataagccaga ggctcttcta
     1501 tgagaatatg gggacc
//
LOCUS       DAETPZ010000266         1405 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.272, whole genome shotgun sequence.
ACCESSION   DAETPZ010000266 DAETPZ010000000
VERSION     DAETPZ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.272"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            106..435
                     /locus_tag="K0W59_004377"
                     /pseudo
     CDS             106..435
                     /locus_tag="K0W59_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(478..678)
                     /gene="glgS"
                     /locus_tag="K0W59_004378"
     CDS             complement(478..678)
                     /gene="glgS"
                     /locus_tag="K0W59_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HBI3025077.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRLVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            1000..1405
                     /locus_tag="K0W59_004379"
                     /pseudo
     CDS             1000..1405
                     /locus_tag="K0W59_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          395 a          326 c          311 g          373 t
ORIGIN      
        1 gacgagaagg ggaagggcct tcccgagagt ggtaaaggag aggccctgga aataccccac
       61 agccccgaaa aagagccagt cagttgctta actgactggc attacaccgt tagtgagacg
      121 tttagtgtag tcatgacaaa agatcctggt gcggcctgta ctgagcaatt caatatttta
      181 gggagctttt tcactacgga tattttgagt gattatagcc atctggatat gggtaatgga
      241 ctgctattga agatatttca taaagatgga acagcaacgg aatttaaccg cttctcacaa
      301 tttgcttctt tttcatcgtc tagtgcacct tcggtcaccg caccattcag ggcagaactt
      361 agtgcgaacc cggcagagac tgttgttgag ggaccgttta gtaaagacgt aatcctcaaa
      421 atcacctata actagcatct aatacaaaca ctaaaacggg ccatcaggcc cgttatatca
      481 gtgctctaac tccagctctc ttgcctgcat catctgttgg caataccagg cataacgtgc
      541 gcaaaaccag gttctatgtt cttcatccat gttaccagta acggccagaa tatcgaccag
      601 gcgaccttct gcatgcattc tggcaaaact acgcgccagg aaatcgaaat tattcaaaga
      661 attaagacta tgatccataa gcacatcctc cggttatcgc atacctttta aggccatatg
      721 ctttttctga tttcagtatt ccccaatcag ggtagtgcct ttaaacataa cgtgcgtaaa
      781 tatattgtcc ccgatcacac tttttagtga aaaataaaaa accatattaa aatactttct
      841 cttccagagc ttctctaaca tgtgttatta ttaacaaaga aaaatgtaag aagggaaact
      901 attccaaggt aaatattgcg tcactaaatg gacattggag taacggtaat gctgccaact
      961 tactgattta gtgtatgatg gtgattttaa ggtgcttgcg tggcttccat ttccatcaga
     1021 tgtccttcct gctccgctac tgaaggcgtg gtgcgtaacg gcaaaagcac tgccggacat
     1081 cagcgctatc tctgctctca ttgccgtaaa acatggcaac tacagttcac ttacaccgcc
     1141 tctcagcccg gtaagcacca gaaaatcatt gatatggcca tgaatggcgt cggatgtcgc
     1201 gccagtgcac gcattatggg cgttggcctc aacacggttt tacgtcactt aaaaaactca
     1261 ggccgcagtc ggtaacctcg cgcatacaac cgggcagtga tgtgattgtc ttcgctgaaa
     1321 tggacgaaca gtggggctac gtcggtgcta aatcacgtca gcgctggctg ttttacgcgt
     1381 atgacaggat acggaggacg gttgt
//
LOCUS       DAETPZ010000267         1395 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.273, whole genome shotgun sequence.
ACCESSION   DAETPZ010000267 DAETPZ010000000
VERSION     DAETPZ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.273"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..171)
                     /locus_tag="K0W59_004380"
     CDS             complement(<1..171)
                     /locus_tag="K0W59_004380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3025078.1"
                     /translation="MALPFAADACLVASSFEADADDADDAAEPADVLAARAEPAASVA
                     LVAASLADVPASL"
     gene            308..>1395
                     /locus_tag="K0W59_004381"
     CDS             308..>1395
                     /locus_tag="K0W59_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HBI3025079.1"
                     /translation="MEDASTTKKGIVQLSSATNSASESLAATAKAVKVVMDETNKKAP
                     LNSPALTGTPTTPTAPKGTNNTQIASTAYVMAAIAALVDSSPDALNTLNELAAALGND
                     PNFATTMTNALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAK
                     STVAAVIEYLGLQETVNKADNAVQKTGDTLSGGLTFENDSILAWIRNTDWAKIGFKND
                     SDADTDSYMWFETGDNGNEYFKWRHRLAGGQLKELMNLKWDSLNILVNAVINGCLGIG
                     TTNALGGNSIAFGDNDTGLKQNGDGLLDVYANGQHVFRFQNGVAIAFKNIQAGTARKF
                     TLSSANNSTKNAAFYLWGNPSRPVVAELG"
BASE COUNT          365 a          344 c          398 g          288 t
ORIGIN      
        1 cagggatgcc ggcacatctg ccagtgaggc ggcaaccaat gcgactgatg ctgcaggctc
       61 agcacgcgca gccagcacgt cagccggttc ggcagcgtca tcggcatcat ctgcgtctgc
      121 ttcaaaagat gaggcgacta aacaggcgtc agcagcgaag ggcagtgcca cgacggcatc
      181 ctctaaggcg acagagtctg caggcagtgc gacggcggca gcgcagagca aaagcgcggc
      241 agaatccgcg gcaatgcgcg ctgagacagc agcaaaacgg gcagaggata ttgctgatgt
      301 cgtatctctg gaagatgcga gcacgacgaa aaaggggata gtgcagctca gcagtgcgac
      361 caacagtgca tccgagtcac tggccgcgac agcgaaagcg gttaaggtgg taatggatga
      421 aacaaacaag aaagcgcccc tgaacagtcc ggctctgacc ggaacgccaa ccacacccac
      481 tgcgccaaag gggactaata atactcagat cgccagtacg gcttatgtta tggccgcgat
      541 tgctgccctc gtggactcgt cgcctgatgc actgaacacg ctgaatgaac tggcggcggc
      601 gctgggcaac gacccgaatt ttgcgaccac catgactaac gcgcttgcgg gtaagcaacc
      661 gaaagatgcc actctgacgg cgctggcggg gcttgctact gcggcagaca ggtttccgta
      721 ttttacgggg aatgatgtcg ccagcctggc aaccctgaca aaagtcgggc gggatattct
      781 tgcgaaatcg accgttgccg ccgttatcga atacctcggt ttacaggaaa cggtaaacaa
      841 ggctgataac gctgtccaaa agacaggcga taccttgtcc ggtggactta cttttgaaaa
      901 cgactcaatc cttgcctgga ttcggaatac tgactgggcg aagattggat ttaaaaatga
      961 ttcggatgca gacaccgatt cctacatgtg gtttgaaaca ggtgacaacg gcaatgagta
     1021 tttcaaatgg agacaccgcc tcgctggcgg ccagctcaaa gagctgatga atcttaaatg
     1081 ggattcacta aatattctgg ttaatgccgt cattaatggt tgccttggca ttggtacgac
     1141 gaatgcgtta ggtggaaatt caattgcttt cggggataac gataccggcc tgaaacagaa
     1201 cggcgatggt ctgctggatg tttatgcgaa tggacagcat gtatttcgtt ttcagaatgg
     1261 cgtggctatt gcttttaaga atattcaggc cggaactgcc agaaaattca cgttatccag
     1321 cgccaacaac tccacgaaaa atgcagcgtt ttatttgtgg ggtaatccat ccaggcctgt
     1381 tgttgcagag cttgg
//
LOCUS       DAETPZ010000268         1385 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.274, whole genome shotgun sequence.
ACCESSION   DAETPZ010000268 DAETPZ010000000
VERSION     DAETPZ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.274"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            144..641
                     /locus_tag="K0W59_004382"
                     /pseudo
     CDS             144..641
                     /locus_tag="K0W59_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            682..873
                     /locus_tag="K0W59_004383"
                     /pseudo
     CDS             682..873
                     /locus_tag="K0W59_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(967..1176)
                     /locus_tag="K0W59_004384"
                     /pseudo
     CDS             complement(967..1176)
                     /locus_tag="K0W59_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(1226..>1385)
                     /locus_tag="K0W59_004385"
     CDS             complement(1226..>1385)
                     /locus_tag="K0W59_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3025080.1"
                     /translation="YIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRE
                     RSLEFEKE"
BASE COUNT          381 a          345 c          352 g          307 t
ORIGIN      
        1 cttattatgc ttccatcgga aacgatgatc tggcagcctg agttcacaga taaaacactc
       61 tccaggaaac ccggggcggt tcaaagcgcc agccccgtcc cgcccgatag agcgcggcat
      121 agcgggcccc ggattacttg cgtgcgacgg cagaagaagc cctgaagcga atcagtgaac
      181 tgtacgccat cgaggatgaa atacggggat taccagagtc agagtgtctt gcagtcaggc
      241 agcagcgaag caaagcgtta ctgacgtcgc tgcatgaatg gatggtggag aagaatggca
      301 cgctgtcgaa aaaatccaga ctgggcgaag cattcagcta tgtactgaat cagtgggacg
      361 ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca gcggaaagag
      421 cgcttcgtgc agtctgtctc ggaaagaaaa attacgtgtt cttcggtagc gatcacggcg
      481 gcgagcgtgg tgcactgctg tacgggctga tcggcacctg ccgtctgaac ggtatcgatc
      541 cggaagcgta tctgcgccat attctgagcg tactgccgga atggccttcc aaccgagttg
      601 acgacctcct gccatggaag gtggtgctac catcaggata atcgtcaata cggtgttcgg
      661 tgaacgttta cttggagcat catgcttcat gaagaaaaac tggcacgtca tcaacgtaaa
      721 caggcgatgt atacccgaat ggcagccttc ccggcggtga aaatgttcga agagtatgac
      781 ttcacgttcg ccaccggagc accgcagaag cagctccagt cgttacgctc actcagcttc
      841 atagagcgca atgaaaacat agtcttactg gggacatctg acatcacaaa tccccgggtg
      901 gggatttgtg tataagagac agcccatgga ggaaggagat atatcgcagc tctgacgact
      961 cactccttat ttacccggga aatacttctc cagcaatgca tacaggatgg cagcggtttc
     1021 agcatccatt atgccgtcat aatttttctg ccggaaatga agctgaaaag cgcggacaag
     1081 cgccttatat ccgtcatccg tctccacacc agaaatactg tatccatagg tcttcagccg
     1141 tgacacgata tcaaccttat ccggaatgcc ctgcgattca aaaaataaat atttagtcag
     1201 taattttatg tttttcctga gaaaattact ctttttcgaa ctccagtgag cgttcacgac
     1261 tcatcagtgt gaaaatgatg gcaaaaacag cagacagtat cagtacgata aatgtggccc
     1321 cccatcccag ataatcgaca acaagtccca ttcccagtgt cgcaacaatt gagccgaaga
     1381 tatag
//
LOCUS       DAETPZ010000269         1383 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.275, whole genome shotgun sequence.
ACCESSION   DAETPZ010000269 DAETPZ010000000
VERSION     DAETPZ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.275"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..569)
                     /locus_tag="K0W59_004386"
                     /pseudo
     CDS             complement(1..569)
                     /locus_tag="K0W59_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(617..1111)
                     /locus_tag="K0W59_004387"
     CDS             complement(617..1111)
                     /locus_tag="K0W59_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3025081.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTGQAIAATGV
                     GIRIFNQSDNSQVKLYNDSDYTAIDAEGKAEMKFIARYVATNATVTAGTANADSQFTV
                     EYKK"
BASE COUNT          422 a          278 c          261 g          422 t
ORIGIN      
        1 acactgatat gaattgggtt gggattctct gctatcagat ttcgcccttc ccggcgccat
       61 ttcactttcg gcgcagcatc ttcgggaata aggcggatat ttgctggtcg ccagaacagc
      121 ttgatacgcg tttttactgc tatttccagc gagttcgtcg ctttgttgct cgctgatggc
      181 ggaatatttt taatattcag ccagaagact gactcgcgat catcaggcaa tgacacacct
      241 ttggtgtgaa taatgcgtaa tattttttct tgcgaaggtt ctaatcgtga tactggcgga
      301 ataacggtaa acggcgcttt gcttttatcc tctttattaa atggatctac ccaagcctgg
      361 atgagataag gtattttttc ttctttgcta aatacagaaa tagatttatc agcattatta
      421 cttaagtaaa ccacccgcgt actaccaatg acaacgcctg cataagttaa tgctggttgg
      481 cctaaaaaaa cgctgagtat aatcgcggta aataacttac attaaactgt tgttttaaag
      541 agcctgatta aacaactttt tctggacata acgactccct ggccaaagcc aattgaataa
      601 aaaggccctt gataaattat ttcttatatt cgacagtaaa ttgtgaatca gcgttcgccg
      661 taccagccgt tacggtcgca ttggttgcca catagcgtgc aataaacttc atttcagcct
      721 tgccttcagc atcgatagcg gtataatcag agtcgttata aagtttaacc tgcgaattat
      781 cggactggtt aaaaatacga ataccaacgc cggttgccgc aatcgcttgt cctgtaccat
      841 caaaacgaac ggctgcttga gtgtacgttg ccgggcaatt ttcaagttta atactgaact
      901 tctgtggcga cgccagacta cctacacctg aaaatgcatt tttactgact ttacccagtg
      961 ggacaatttg atcggctgta tcggaggtaa cttcacaagt agtgtcgaca atttcaccga
     1021 cgaagttaac aacaccatct tctgccagtg catgcccggc cagcattaga gatgccagag
     1081 tgaatgcacc gcctataata ttacgcttca taaaaactcc attgatataa tatatccttt
     1141 accatgaatc atcacttata ttaataagtg tatttttgat tcaaaggaat agtacaccaa
     1201 agagacggct ttatctaaaa ataatattaa ttacgcaaaa ataaaatata gatttattgt
     1261 ttaaatatta tttaaacagt aaaaaatcaa gtttgttatc agacattaat gataattcag
     1321 aatatattta tatagaaaga tagatgaatt tattgatgtc agttataaaa aagggccacc
     1381 ggt
//
LOCUS       DAETPZ010000270         1333 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.276, whole genome shotgun sequence.
ACCESSION   DAETPZ010000270 DAETPZ010000000
VERSION     DAETPZ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.276"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(7..489)
                     /locus_tag="K0W59_004388"
     CDS             complement(7..489)
                     /locus_tag="K0W59_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI3025082.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDNARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(480..782)
                     /locus_tag="K0W59_004389"
     CDS             complement(480..782)
                     /locus_tag="K0W59_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBI3025083.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1109..1327
                     /locus_tag="K0W59_004390"
     CDS             1109..1327
                     /locus_tag="K0W59_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3025084.1"
                     /translation="MKAVARANNHPYKSVFADFITGHPSCTVCFWETFHKMYPDSPYE
                     YVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          393 a          330 c          284 g          326 t
ORIGIN      
        1 tcagactcag actttataaa acaaggtatt aggtgccaca actgactgaa caaaaccaaa
       61 acgctcataa aatgctctgg cgttatcatc aattgcatgt accataacag ccttaatgcc
      121 aacctgatca gccagattga caacccggtg aatggcatca cttagtagcc atttaccgaa
      181 accatgcccc tgggtacaaa catcaacagc cagacgccct aacagaacaa cagggacagg
      241 attgggcatg ttatgccgca aactgcgacc aagctcggca tgagtgacgg aaccagtagc
      301 taatgagtag tatcccacaa tacgtaaagt atcctccaag caaataacga acgtgcgaga
      361 agcattgagc atctggtttt tcatagcccg accacgtaac cagttactta gcacctcatt
      421 tccacagtca aatggctgaa gtatatgctc atccgtcaac aatgctggcg cggtgacatt
      481 tatttccatt caggctttac cgccatcaga cgttcacgcc cttctgcatt ctgcacagga
      541 gcttccagtt gcgtaataaa cgaatcataa cgttcgttat ccagcacaaa taaacgttga
      601 tccaggataa tgtttttagc ttcctggtac gccagttcca tgatgaaatc ggtacggttc
      661 ttattgacca agctcgcagc ataatcaata acggctcgtt cctcatcagt tgcccggatg
      721 ttaatctgat gttctcgcgg tgttttacgg acgcttgcag ctgtagacat aaaaacctcc
      781 attggtaaca atttgctatg caattaatgt tatcgcaaat ggtaacagaa cgctatacag
      841 aatcacttac gggaaaagcg atcccctgat aaaaacaatg gcagagacct gaacattgtg
      901 acgatgatct gtgtcggcgc acaaatcatg tctgattacg gccgtttaat gtgattttga
      961 taatgaaatg cctgaaccac ggcagcctgg cggctgcaaa tcggggtgtg gcagaatatg
     1021 agctttcaga gtaaccatcc agaatgagtt cccatgtcac acaacctgga gcaccagaaa
     1081 gtccacacca gaatggtgaa agagggttct gaaagccgtc gccagagcca ataatcaccc
     1141 ttataagtcc gtatttgcag actttatcac cggtcatccc tcctgcacgg tctgtttctg
     1201 ggaaaccttc cacaaaatgt acccggattc accgtacgaa tacgtcacgt tctgccatac
     1261 ctgccgtcgt tttgatttat acgaaacaga agcggagatg aaggcggatg acccgaagtg
     1321 gtggtgattt tca
//
LOCUS       DAETPZ010000271         1329 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.277, whole genome shotgun sequence.
ACCESSION   DAETPZ010000271 DAETPZ010000000
VERSION     DAETPZ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.277"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..104)
                     /locus_tag="K0W59_004391"
     CDS             complement(<1..104)
                     /locus_tag="K0W59_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3025085.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLS"
     gene            162..557
                     /locus_tag="K0W59_004392"
                     /pseudo
     CDS             162..557
                     /locus_tag="K0W59_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(558..1202)
                     /locus_tag="K0W59_004393"
     CDS             complement(558..1202)
                     /locus_tag="K0W59_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3025086.1"
                     /translation="MTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSL
                     IDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAATPEYFARYGKPRHPHDLLNHQ
                     CVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYL
                     DTGRLECVLEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          334 a          302 c          314 g          379 t
ORIGIN      
        1 gataatcggg aagcgccttc actgatcgaa agttgatttt caagaaccgt tctgacagct
       61 tcggctttga actctttaga gtaacgttgg gtttttctgc tcattattag ctccttctga
      121 tgccattcta tttcaggaag gagtgtccgt taaactcagg ctacctcacc ctggtgctgg
      181 tggcaattct gattatgctg ccgactctcg gcatcggtga tatgaacaat ctctataaat
      241 ggctgttgaa tcttaactcg gtagtgatgc cgctgcgtta tttatgggta tttgttgcat
      301 ttattgcagt cgttcgcctg gcgcagaaat ataaaccaga atatgtcttt attcgtaata
      361 agcctctggc aatgaccgtc gggatttggt gttttgcctt taccgcctgt gcctgtttga
      421 cggggatctt cccgaaaatg gaagccttca ctgcagagtg gaccttccag ttggcgctga
      481 atgttgcaac gccgtttgtg ctggtaggat taggactgat attcccgctg ctggcgcgta
      541 aagcgaatag caaataatta ttgtggtcgg tgacgctgac tctggcagat ctgacccgtt
      601 tttacataat cgagaaatgc gcgcaagccg caggacatat actggcggtt tgggtaatag
      661 acttggaagc ctggacgctc tgtgatccag tcttccagta cacattctaa acgaccagta
      721 tcaaggtact ccttgatctg ttcatagagc agatacccga taccagcacc catcaaaacg
      781 gcctccagtt cagcatcaac atcatccaga atgatatttc ctgcgacagc aatttccagc
      841 tcctttgcaa attgccagtg gaaaggtttg ccgctgggat aacgaaaaac aacacattga
      901 tgattaagca gatcatgagg atggcggggt ttgccatatc ttgcaaagta ctctggcgtt
      961 gctgctacac ataatttcac tggtgggccg atcgccacgc tgatcatatc tttttcgacg
     1021 atgccactaa gtcgaacgcc agcgtcaaag ccctgttgga cgatatcgat aagagaatcg
     1081 tcagttgtga gctctacttt aatatcagga tattcacgtg tgaaaccaac cagtaaaggc
     1141 attaaaaata tacgtgcagc tacccgggcg gcattgattt tgagcgttcc tgtcggtgtc
     1201 agacgaaaat cattcatctc atctaacatg atctgaattt cgtcgaaagc tgggcgcaat
     1261 cgttcataga gattagaacc cgcttccgtt aacgatacgc ttcgggttgt ccgattgaat
     1321 aagcgaatt
//
LOCUS       DAETPZ010000272         1327 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.278, whole genome shotgun sequence.
ACCESSION   DAETPZ010000272 DAETPZ010000000
VERSION     DAETPZ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.278"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..921)
                     /locus_tag="K0W59_004394"
     CDS             complement(<1..921)
                     /locus_tag="K0W59_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HBI3025087.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITE"
BASE COUNT          404 a          198 c          271 g          454 t
ORIGIN      
        1 ttcagtgatg actctgatat ggcgcgaatg taaattttgc ggcaacatct ggcgtaaaaa
       61 cagtctgttt tggtctgaca gggaaatacg aaactgaggc ccgttaaaat ttggaaccga
      121 agtttgctgt attaagaata gcagagtttg atctgataag gggttatttg caatctcaat
      181 agttatatga ggatctaaac gaaaaagatt tgtcgggatt gtagttattt ggttgttttc
      241 aaaataaaaa aatcttctca tgataatatt ttcttggatg tttggtaagt tgcttaatct
      301 attgctatat gcaaaaagaa gttgcagtct cctaggtaaa gggggcagtt cagtaagatg
      361 gttgtgcgca accttaagta tcttaagatt agaaggcaga gatggaagag tttgcagttc
      421 gttgttccta acctcaagtt tttttaaatt caagggtaat gaaggcagtg tggataacct
      481 attatactct gcgcttagtg atttgatggt gtctggtaat aacggcagat tctctaattg
      541 attttcggac acatccaaag ttattagata atgtggtaat gcagatatat gtgttaagaa
      601 attatctctt gcaataagtt ctttaatatt ttcaggtaaa tcagtaagga tggttaattt
      661 attacagctc acatcaagct ttttaatgca agcagggagt acaggtaacg aggaaagcgc
      721 caaggctcgc aggtccagtt tatgcccctt agaggtaaga catgcacgaa gtcttcttac
      781 cgcctcagta cgctgttctt ccggtaaccc ctgtttctcc caatcatccc attcggacaa
      841 ataatggttg ggggatgtaa ttgtgctgat atgtggaaat aaacaggatg aaatatttct
      901 gagcatatta atttctcgca tatggatatg gaattagatt gtgaataaat tgtatttaat
      961 gtatctctcc taatgaggag ggtgtttcag aatattcgcc ataagggaat gagtgttata
     1021 tcatgctttc taccctggta agtatatttg cttttttcgt atatttggtt catctttttt
     1081 tgatgtttta catattcaca atgcagagga tttataccag aagacattag tcttgagtaa
     1141 gatactacaa catctccaac tattttagcc agcctgacta cttgacttgg taatatttgt
     1201 tgctaataat catgtataaa ataagcttat atacatagta acttcagcaa aggatttagt
     1261 aatgatccaa atcatatctc aatgtactgt ttttttcttt aaaaaaacag tacattctat
     1321 tttataa
//
LOCUS       DAETPZ010000273         1310 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.279, whole genome shotgun sequence.
ACCESSION   DAETPZ010000273 DAETPZ010000000
VERSION     DAETPZ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.279"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..866)
                     /gene="ipaH4.5"
                     /locus_tag="K0W59_004395"
     CDS             complement(<1..866)
                     /gene="ipaH4.5"
                     /locus_tag="K0W59_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HBI3025088.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GPQIYFSMSDGQ"
BASE COUNT          368 a          236 c          292 g          414 t
ORIGIN      
        1 tgttgtccgt cactcatgga gaagtaaatc tgcgggccgt ggtagtccgg cgaagaggtt
       61 aatctttgca gggattgcag aacgtgagtc gacaatggat tccctgaaat gttaacattc
      121 gtacaaatgg gcaatgtgat tatattttcg ggtaatgaag ttagtaggtt attggaaaca
      181 tccagtgcca atagttcttg cggtaatctg gataatctgc gtagttgatt ttcacctacc
      241 ttcaaatatt ttaagctctg aggaaattct ggtagttcct gtagtctgtt gccggaaaca
      301 aagagttctt gtagtctatg aggcaattca ggcaagacag taaggcgatt accttcaata
      361 cgtaatattg ttagcgttga tggaagctct ggtaacgtct ccagactatt aaaacgtgcc
      421 gagagtgatt gtaaatatgg tggtaatgat ggcagacatg acaaatttcg attgaatgaa
      481 atattaagct taatcagttg agatggaagt gtcgggagta tattcaagtt gttaccattt
      541 acatgaagtt ctgtcaaaag cggagaattt tcaggtaggg agattaactc attgttggaa
      601 atattaagtt cacgtatatg caaagggagt ggtggtaaag aacgtagttt cagtaagctt
      661 aaattgagaa caggctcccg ggtatccaga catattttca ataatcgaac cgcctggatg
      721 cgattttctg ttgttgttcc ttccctagcc cagtcatccc agatgcggtg gtaatctctg
      781 gtacactgtt cttctaccga taaacgagat atacatgata agccacaaag ggaacgaaaa
      841 aaagaatgat tgttgatcgg tttcatttat taacccctca ctattcatat cttagtctat
      901 taatgcaaat aaaacgagaa agtctttaaa aaaacaatcc tagagcttta tcggcgtttt
      961 cattccggct ggaggaatat gttcctgtct ttccagtaac cgtcgattgt tatatcagtc
     1021 tacccatgtt aatgtgacca gttctacttt catccagttt ggattgatct ttatccccta
     1081 tattttcaaa caatttttat catttaactc attactgtct cgatattccc atagcaaacg
     1141 atctggttgg ttatcccctc aaaaaagtca gttgcacgcc gtaaatccct gccggtattg
     1201 agcgaggatt tacgggttgt tgttaaggtc cgtaatcggg tcacagtttt tccggagtca
     1261 atccggtctg cggtttatgc ttatgcgacg tgattatgaa tggtgcagtc
//
LOCUS       DAETPZ010000274         1299 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.280, whole genome shotgun sequence.
ACCESSION   DAETPZ010000274 DAETPZ010000000
VERSION     DAETPZ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.280"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..226
                     /locus_tag="K0W59_004396"
     CDS             <1..226
                     /locus_tag="K0W59_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3025089.1"
                     /translation="MSRKGNCYDNAPMESFWGTLKNENLSHYRFNNRDEAISVIREYI
                     EIFYNRQRRHSRLGNISPAAFREKYHQMAA"
     gene            complement(268..1299)
                     /locus_tag="K0W59_004397"
                     /pseudo
     CDS             complement(268..1299)
                     /locus_tag="K0W59_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
BASE COUNT          386 a          329 c          284 g          300 t
ORIGIN      
        1 aatgtcgcgt aaaggtaact gttacgacaa cgctccgatg gaaagcttct ggggaacgct
       61 gaaaaatgag aacctgagcc actatcgttt taataaccgg gatgaagcca tctcagtaat
      121 acgggaatac attgagattt tctacaatcg tcagcgtcgt cactctcgtc tggggaatat
      181 ctccccggca gccttcaggg aaaaatatca tcagatggct gcttaaaaaa agaacaaatg
      241 gtagtgtccg ctattgccag tacacctcag ggtcagaaaa tagccattaa cgggcgtacc
      301 agaagcgttg gtacgacata aactggctgg aatatatttg ctgtcagcat cacctaatag
      361 cactttaagc ggggcatcaa tcgagaatac cagcgcagct acttgtccca gggtattcgc
      421 aatggcgtaa atcaccatta aagtattacc catgttgtaa tactcgccca gcttctgaaa
      481 ggcgtaatat tgaccgttcg tcattaagtc atccgggata ttacgcgagt caaacatcat
      541 ccccatcgcc agtgagccca gaatggcaca aaccgcaacc atcaccgcca ggcatagcat
      601 cccttttgga aattcttttc ctgggttgcg cgtttgatta acgtaaggag aaatcttctc
      661 tgctccgcca accgcgaaaa ccagcattga aatagtggta atgtaggtaa agtcgatatg
      721 aggaatgaac gtttcccagg taatgtttgt ggtcgcaata tgcacttcag taatcgcagg
      781 cgcggttacc gccatcgcca catacaggag cgacataaca aacatcgcaa tccctgccac
      841 agaaccgacg atttttagcg atttcatacc gcgtgaagca acccacataa agaagataaa
      901 cagcaccagc gttaacccct gtaacgctac gactgaatat tctttgatta gcgaaccgtc
      961 gcctttcatc gcccaaccga gcgcaatcag aattgcctgg ggtttttgtg ccagataggg
     1021 aatatgcacc acccagtagg tccacgcggc gagataagcc agtccgggtc ccatcgtgtg
     1081 tttaatccag gtactgaccc cgcccttccc atctttgaag gtcgagccta actgaccaac
     1141 aattagcgca taaggtgtga aatagagtgc aaagataaac acccatgaaa aaacaaccac
     1201 cagcccctgg ttggcatagt tattaacaac gttgccaaaa ccccagacag tgataaaaga
     1261 catcagcgca atgttgtacc atcgcaactg tttttcctg
//
LOCUS       DAETPZ010000275         1296 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.281, whole genome shotgun sequence.
ACCESSION   DAETPZ010000275 DAETPZ010000000
VERSION     DAETPZ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.281"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..815)
                     /locus_tag="K0W59_004398"
     CDS             complement(<1..815)
                     /locus_tag="K0W59_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBI3025090.1"
                     /translation="MKPVHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="K0W59_004399"
                     /pseudo
     CDS             907..993
                     /locus_tag="K0W59_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          363 a          219 c          336 g          378 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtggacaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaaatg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcggt cagctt
//
LOCUS       DAETPZ010000276         1276 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.282, whole genome shotgun sequence.
ACCESSION   DAETPZ010000276 DAETPZ010000000
VERSION     DAETPZ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.282"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..769)
                     /gene="ospC4"
                     /locus_tag="K0W59_004400"
     CDS             complement(<1..769)
                     /gene="ospC4"
                     /locus_tag="K0W59_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HBI3025091.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNVLRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNLSNNILNIKSFD
                     KIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEV"
BASE COUNT          376 a          219 c          236 g          445 t
ORIGIN      
        1 caacctcttt aaagttatct aagaaaaatg attggtgctc atggtaaaaa acaggtggaa
       61 tagcattatc aaagtgatcg gttaatgtca tatatccata tggagagtca tgatcaatga
      121 tatacgcatt ggcgccaaaa tctacagtat gatgtgtttt atttagtggg agtttttctt
      181 gatgattgga aatttcaacc ccaaaaaaaa caaaatcgtg gttagagatt tctttaatat
      241 cctcagagta tgtattctta tgtgtttgta tattttcaga ctgaatttta tcaaaggatt
      301 taatattaag aatattattt gacagattag agttactttg atgtctgaag ttaaaattag
      361 aattaataat aatagaaagt aagtgacgtt cgtctgaaga aagaggatga actttttgta
      421 ttatctcaga gatatagtgc ctggacacct tatcgagatg ctcaagtgac cgaaatgatg
      481 accgtgctcc agatggcgct tcaatagaaa gatttagtga tttgaaactg gtcccttgag
      541 aaaacatttt gctataactt tgcgctgcta tagacttcct taaaaagttt ttcacagtat
      601 tagcacagtg atctaaatgt ttttcactta tgccagaaga agatgagttt gttacgcgta
      661 atacattttt ttgtaatgct tttgttgctt tattcggatt cgtttttcca tcaacaagat
      721 caatattatt ctgaacatta atatgattta ctgcttcagg tattttcatc cccccccccc
      781 aattatgtgc gtttttatct gtggttattt agataacatt atctaactgg acactttctt
      841 gtatgtatta tagctaaatg aacagatttt aatatgttgt atggttttca ctctgaaatc
      901 ccctgaattt aatcacgttt cctttactga aggcaatttt aaccgtagta tcaattatta
      961 acccttgggc ttgatatccc tctcctcttc tgggcacggg gaatgtcaga ttacttatta
     1021 cagaaaccaa aagaattgta agcgtcatct cagtgtcgtc tatttcaatg tctgatgatt
     1081 ggcgatgata gagtcctctt ataacgttaa tggactctat caatgtcaga ttctcttcgc
     1141 ccccgcagga ttcgtgcctc ttactctatg actctatgga gtttaagctt gatcttattg
     1201 aaaaatctta ccagctaggg gcctgcgtgg cccaactggc cagagagtac gggattaatg
     1261 taagcgcccc atcagc
//
LOCUS       DAETPZ010000277         1264 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.283, whole genome shotgun sequence.
ACCESSION   DAETPZ010000277 DAETPZ010000000
VERSION     DAETPZ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.283"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(238..312)
                     /locus_tag="K0W59_004401"
     tRNA            complement(238..312)
                     /locus_tag="K0W59_004401"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(277..279),aa:Gly,seq:tcc)
     gene            complement(316..391)
                     /locus_tag="K0W59_004402"
     tRNA            complement(316..391)
                     /locus_tag="K0W59_004402"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(356..358),aa:Thr,seq:tgt)
     gene            complement(394..470)
                     /locus_tag="K0W59_004403"
     tRNA            complement(394..470)
                     /locus_tag="K0W59_004403"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(434..436),aa:Arg,seq:tct)
     gene            complement(472..548)
                     /locus_tag="K0W59_004404"
     tRNA            complement(472..548)
                     /locus_tag="K0W59_004404"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(512..514),aa:Ile,seq:cat)
     gene            complement(757..1263)
                     /locus_tag="K0W59_004405"
     CDS             complement(757..1263)
                     /locus_tag="K0W59_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBI3025092.1"
                     /translation="MPVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGND
                     LKELRVALNYSQNPVSPKARSLILWTERGAARHAKMLETDQAWDVFEKLEDCYFSQRD
                     PSTPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDK
                     LVERLMQL"
BASE COUNT          344 a          316 c          292 g          312 t
ORIGIN      
        1 gcttagaata ttttgtaagt tgtccgaaga gatatttaca acaccagaat gatgcattat
       61 cagtcccctg ccagaggttc actacgtaac ttgaccgaca atgcaccatt ctggtgctga
      121 aaaaagagca ctggaactgc aacaaaacaa catcacagaa caggagatgg taaggagtca
      181 gacattccca cacaatattg tgtcagtgca aataacaacc ttcgtctcaa atctcgctgg
      241 agcgggcagc gggaatcgaa cccgcatcat cagcttggaa ggctgaggta atagccatta
      301 tacgatgccc gcatatggtg ccgactaccg gaatcgaact ggtgacctac tgattacaag
      361 tcagttgctc tgcctgctga gctaagtcgg cgctggccca ccaccgagga ctcgaacctc
      421 gcaccgtcaa cttagaaggt tgatgctcta tccggatgag ctagtggtgg ttggtggccc
      481 ttgctggact tgaaccagcg acctggcgat tatgagtcgc tcgctctgac caactgagct
      541 aaagggccgg aggcagaata ataaccatat gtcatcacat ctgcaaactc atctgaccac
      601 cagcgcgttt aacgccctgt gccatttttc aggcacaaaa aaacccgcat aaagcgggtt
      661 ctttcaagtg tccatgtctg ctattcgcct cgcgatacag ctttgcgaag cttaccggaa
      721 ttgaagcagt ttatgtgcaa aaatgcaaga acttttttaa agctgcatca acctttccac
      781 cagtttatct ctgcgaacaa caaaccaacc attggctctc gccagttcca gccatgactc
      841 aagggaaata acaatatcat catcccgtaa ctgaattgtg gaaacagtga caccgcctct
      901 ctgataacag agaactcgcg tgtcgtaact tttctggcat gaaactggcg ttgacggatc
      961 cctttgactg aaatagcagt cttccagttt ttcgaacaca tcccacgcct gatcggtttc
     1021 gagcattttg gcgtggcgtg ctgcgcctcg ttctgtccag aggatgaggg agcgggcttt
     1081 gggtgaaact ggattttgtg agtagtttaa agcgacccgc aattctttaa ggtcattacc
     1141 aacaactttg aaaaagtgtt tcccttcaac gaagcgtact ttgttctcat gatgattctg
     1201 gcgaatacgc accggctcag tgccgtaaag ctgcgccaaa agttcggtgg taataacagg
     1261 aatc
//
LOCUS       DAETPZ010000278         1227 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.284, whole genome shotgun sequence.
ACCESSION   DAETPZ010000278 DAETPZ010000000
VERSION     DAETPZ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.284"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..884
                     /locus_tag="K0W59_004406"
     CDS             <1..884
                     /locus_tag="K0W59_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI3025093.1"
                     /translation="PTISLSAVIYFPPKFDETRQYPAIVVSHPGGGVKEQTAGTYVEK
                     LAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTTLSYVDNTRIGAMG
                     ICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSIDALPHIEAGSNART
                     SDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPGYATLRSLNQIITY
                     DAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIVEGANHMDLYDGKA
                     YVAEAISVLAPFFEETL"
     gene            884..>1227
                     /locus_tag="K0W59_004407"
     CDS             884..>1227
                     /locus_tag="K0W59_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI3025094.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGVLGV"
BASE COUNT          325 a          279 c          317 g          306 t
ORIGIN      
        1 atccaaccat ctctttgtct gcagtgatct atttccctcc aaaatttgat gaaacccgtc
       61 agtatccggc aattgtggta tctcacccag gtgggggcgt taaagagcaa acggccggaa
      121 cctatgtcga aaagctggcg gaaaagggat ttgtcactat tgcttatgac gcatcttatc
      181 aaggtgaaag tggcggcgag ccgcgtcagt tagaaaaccc atacattcgt actgaagacg
      241 tcagtgccgt gattgattac ctgacaacgc tttcttatgt cgacaatacc cgaattggtg
      301 cgatgggaat ttgtgccggt gcaggatata cagccaatgc tgcaattcag gatcgccgca
      361 tcaaggccat cggtaccgtt agtgcggtca atattggttc gatgttccgc aatggttggg
      421 aaaacaatgt gaaatctatt gatgctctgc cacacattga agctggctca aacgcccgaa
      481 ccagcgatat tagcagcgaa gaatacgcca ttatgccact ggctccaatg aaggaggccg
      541 atgcgccgaa cgaagaactg cgtcaggcct gggagtatta ccacacacct cgtgcgcagt
      601 atccaacagc accgggttac gctactttgc gcagccttaa ccagattatt acctatgatg
      661 cttaccatat ggcggaagtg tacctgactc agccgatgca gattgtggcg ggcagccagg
      721 cagggagcaa atggatgagt gacgacctgt acgatcgggc atcaagccag gataaacgct
      781 atcacattgt tgaaggcgca aaccatatgg atctttatga cggcaaagcc tatgtggctg
      841 aagcaatttc agtattagct ccgttctttg aggaaactct gtaatgaaac atattcaaat
      901 ccgcaatagt gatatggcct ggcatattgc tgccaatatt cagtttccac caaattttga
      961 tgagtcaaaa cagtatccgg cgattatcag cgtccatcct tttggcagtt gtaaggagca
     1021 gacgtctggc aatatctatg gcaaggcatt ggctgaaaaa ggctatgtcg ttctcgctta
     1081 tgacgccagt ttccagggag aatctggtgg ggagcctcgt tggatagaag atccgacaca
     1141 gcgcgtagag gatattagcc gtgttatcga ttatgcagta actctgccat atgttgatgc
     1201 tgaacgcatt ggcgtgctgg gtgtgtg
//
LOCUS       DAETPZ010000279         1210 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.285, whole genome shotgun sequence.
ACCESSION   DAETPZ010000279 DAETPZ010000000
VERSION     DAETPZ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.285"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            136..>1210
                     /locus_tag="K0W59_004408"
     CDS             136..>1210
                     /locus_tag="K0W59_004408"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HBI3025095.1"
                     /translation="MQREKTPEWREKQKSSRGIRRGQRYRLVFQFPIRERYIARLRNR
                     VENRLWHSLAACIDDSQTQQLLDLLSVPAGSRYSLLDQLRAGPTKVNATSLVQAIGRL
                     QTIRSLGVTLPAITPVSDIRIAAMARYASTAKITALQRLPEKRKLATLVAFSCCMEAT
                     AQDDALELLEALLRDLFNEAVQADKRNRQRTLKDLDRAAEILAKACRMLLDDKLSDTD
                     VRDSIFNIIPEDVLTHAVNNVTSIIRPDNNVYFNELDSKFKTVRRFLPDLLSRIHFEG
                     NASAKTLIEALCWIEVNLKKKKTDNDAPREIINKPWQQHVIRKDGSIDFHAYTFCALK
                     ELQLALKKRDIYVNPSWRYADPRA"
BASE COUNT          362 a          262 c          280 g          306 t
ORIGIN      
        1 ctgaatgtgg aggggctgaa gaaaaacgcg cgggatatca gtctgctgag gtatatgccg
       61 gcctgacaca ggctaagtgt gcccgggaat cggtgcaagt ataaaaaaca gcctgttgcg
      121 gtataagaat cgaccatgca gagagagaaa acaccggagt ggagggaaaa gcagaaaagc
      181 tcacgtggca taaggcgtgg acagcggtat cgtctggttt ttcaatttcc tatacgtgaa
      241 cgctatatag cccgattacg caacagagtt gaaaaccgtc tctggcattc actggcagct
      301 tgcattgatg actctcagac tcaacagctt ctggatcttc tttcggttcc tgccggaagc
      361 cgctattcgt tacttgatca attacgcgcc ggacctacta aagtgaatgc cacatcactg
      421 gtacaggcta taggtcgcct tcagacgata cgtagcctag gagtgacatt acctgcaatt
      481 acgccagttt cggatatccg tattgcggcc atggcacgtt atgcctcaac ggctaagata
      541 acagcgcttc agcgcttacc agaaaaaaga aaactggcta cgcttgttgc attctcttgc
      601 tgcatggagg cgacagctca ggatgatgct cttgagttac ttgaagcgct actccgggat
      661 ctgtttaatg aagcggttca agcagataaa cgtaaccgcc aacgaacact taaagatctg
      721 gatcgtgcag cagaaatact ggcaaaagca tgtcggatgc ttcttgacga taaattatct
      781 gatactgatg ttcgtgatag tatcttcaat atcattcccg aagatgtact gactcatgca
      841 gtaaataatg tgacatcaat tattcgacca gataataacg tttacttcaa tgaacttgat
      901 tctaaattca aaacggtgcg gcgtttctta cctgacttac tttccagaat acattttgag
      961 ggcaatgcct cagccaaaac attaattgaa gcgctttgct ggattgaagt taatttaaaa
     1021 aagaaaaaaa cagataacga tgcaccacgc gaaataatca ataaaccgtg gcaacagcat
     1081 gtgataagaa aagatggtag tattgatttc cacgcctata cattttgtgc acttaaggaa
     1141 cttcagcttg cactgaaaaa acgggatatt tatgtcaatc ccagctggcg ttatgcagat
     1201 ccccgggcag
//
LOCUS       DAETPZ010000280         1210 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.286, whole genome shotgun sequence.
ACCESSION   DAETPZ010000280 DAETPZ010000000
VERSION     DAETPZ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.286"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(13..753)
                     /gene="cysQ"
                     /locus_tag="K0W59_004409"
     CDS             complement(13..753)
                     /gene="cysQ"
                     /locus_tag="K0W59_004409"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HBI3025096.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            943..>1210
                     /locus_tag="K0W59_004410"
     CDS             943..>1210
                     /locus_tag="K0W59_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HBI3025097.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSA"
BASE COUNT          280 a          327 c          297 g          306 t
ORIGIN      
        1 tgccatctga atttagtaaa tggacactct gaaccccgga ttcaggaacg actcgcgcgg
       61 agtgtaatcc agcggtttac cctgccagtc gtgaacgtgc gctccggcag ctgcagccac
      121 agcatgtcca gcggcggtgt cccaaatatt cgttggtccg aagcgcgggt acagctgcgc
      181 ctgtccttcc gccaccaggc agaatttcag cgaagagccg atggacgtgg tctgatgttc
      241 gccaagctgt tgcagatact ctttcagctc cgcatctgca tgggaacggc tgataaccac
      301 cagcggcggg cgcgcatcgc ggacctgaat cagcttgcgc acaccgcact cttctttcca
      361 ggccttgcct tctgccgcgc tgtacattac gttcattacc ggcgcataca ccacacctaa
      421 aatcggtttg ccatggtcaa tgagcgcaat gttaacggtg aattcgccat tacgtttaat
      481 aaactcttta gtaccatcca gcgggtctac cagccagtaa cgctgccagt gctgacggac
      541 ttcccaaccg ggaggatctt cttcagaaag gaccggaata tccggtgcca gcgtacgtaa
      601 accgtccatg ataacggtgt gagcggcaat atccgctgcc gttaccggag aattgtccgc
      661 tttgctgacg acgtccatcg gtttcgtccc gtcgtagacc tgcataatgg catcgcctgc
      721 attccgtgca agctggcata cttgatctaa catttctcca cctcgtctct gtgagcggtg
      781 ttaacttatt gttttactta taccctatcg ttaatgaatg cgccaactgt gatagtgtca
      841 tcattttcaa agcgtaaaat tgtggcattc ttcactgttc tataagtaag gcgtttattc
      901 tcccttttct ttcgtattcc cgatgataaa aggatgtccc tgatgattaa gtttagcgca
      961 acgctcctgg ccacgctgat tgccgccagt gtgaatgcag cgacggtcga tctgcgtatc
     1021 atggaaacca ctgatctgca tagcaacatg atggatttcg attattacaa agacaccgcc
     1081 acggaaaaat tcggactggt acgtacggca agcctgatta acgatgcccg caatgaagtg
     1141 aaaaacagcg tactggtcga taacggcgat ttgattcagg ggagtccgct ggccgattac
     1201 atgtcggcga
//
LOCUS       DAETPZ010000281         1207 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.287, whole genome shotgun sequence.
ACCESSION   DAETPZ010000281 DAETPZ010000000
VERSION     DAETPZ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.287"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            14..925
                     /locus_tag="K0W59_004411"
     CDS             14..925
                     /locus_tag="K0W59_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI3025098.1"
                     /translation="MINEGDKSQEDIADEAGMSPATLSALRKGEYNGNVEGMHDKLQK
                     WYQVWQKGQNLSGTPGVVKTQIFCDLHELFKTVRSLGIISVVVGVPGVGKTFAAREYC
                     RRTANAWMVTLAPAHSSVTECLLEIAYALEINNPGRNKGNITRAIRRKLGGGKETSGR
                     VNPLLIIDEADHLSVDGLEQLRAIQDATGVGMVLIGNPKQMADATRRGTDELARLFSR
                     FAMTKQLRKVKKTDVMAVAKAWGVTQEDELDLLMRVAEKPGGLRVLTHTLNHAWLAAK
                     GAGTSLNKSHIKAAFKEAYSNPRLLNW"
     gene            941..1168
                     /locus_tag="K0W59_004412"
     CDS             941..1168
                     /locus_tag="K0W59_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3025099.1"
                     /translation="MMSRNIKMATDAQNWLQAHGSHVNESYLGVARPVLEITCPPPEL
                     VRNAVRIMEHKSGVARSVWTARLNGCQIIWR"
BASE COUNT          340 a          256 c          336 g          275 t
ORIGIN      
        1 cactattaac gctttgatta acgagggcga caagtctcag gaagatattg ctgatgaagc
       61 cggaatgtct ccggcaacac tctcggctct gcgtaaagga gagtacaacg gtaatgtcga
      121 aggtatgcat gacaaattac agaaatggta tcaggtctgg cagaagggac agaacttgtc
      181 aggtacccct ggtgttgtga agacccagat attttgtgat ttgcatgagc tgtttaaaac
      241 agtgcgctca ctcgggatta tcagtgtcgt tgttggcgta ccgggtgtcg gaaaaacctt
      301 tgctgcccgg gaatattgtc gcaggacagc taatgcctgg atggtgactc ttgctccggc
      361 acattcgagc gtaacggaat gcctccttga aatagcatat gcgctggaaa ttaataatcc
      421 gggaagaaat aaggggaata taacccgcgc cattcgcaga aaactgggtg gtggtaaaga
      481 aaccagtggc cgtgttaacc cccttctgat tattgatgaa gctgatcact taagtgtgga
      541 tggtctggag caattacgcg ccattcagga tgccactggc gtggggatgg ttttaattgg
      601 caacccgaaa cagatggcgg atgccacccg tcgtgggact gatgaactgg cccgtctgtt
      661 cagccgtttt gccatgacaa aacaactgcg caaagtaaaa aagaccgatg tgatggctgt
      721 agccaaagca tggggagtaa cacaggaaga tgagctggac ctgctgatga gggtagccga
      781 aaagcccggg ggattgcgtg tattaactca cacactgaat catgcgtggc tggcggcaaa
      841 aggtgcaggc acatccctga ataaaagtca cattaaagcg gcttttaagg aggcttacag
      901 caaccccagg ttactgaact ggtagtaaag gacataaacc atgatgtcac gaaatataaa
      961 aatggcaacg gatgcgcaga actggttaca ggcgcacggg agtcacgtaa atgaatcata
     1021 tctcggcgtg gcgcgtccgg tacttgaaat cacctgccca ccaccggagc tggtcagaaa
     1081 cgctgtcagg attatggagc ataaatcagg ggttgcccgt tcagtatgga cggcccgcct
     1141 taatggctgc cagattatct ggcgttaata acggcttatt tatttgtgag gaataacaaa
     1201 atggcaa
//
LOCUS       DAETPZ010000282         1185 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.288, whole genome shotgun sequence.
ACCESSION   DAETPZ010000282 DAETPZ010000000
VERSION     DAETPZ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.288"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>1185
                     /gene="amn"
                     /locus_tag="K0W59_004413"
     CDS             <1..>1185
                     /gene="amn"
                     /locus_tag="K0W59_004413"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HBI3025100.1"
                     /translation="AIGKYITIGELPDENARKQGLFVYPSLTVTWDGSTTNPPKTRAF
                     GRFTHAGSYTTTITRPTLFRSYLNEQLTLLYQDYGAHISVQPSQHEIPYPYVIDGSEL
                     TLDRSMSADLTRYFPTTELAQIGDETADGIYHPTEFSPLSHFDARRVDFSLARLRHYT
                     GTPVEHFQPFVLFTNYTRYVDEFVRWGCSQILDPDSPYIALSCAGGNWITAETEAPEE
                     AISDLAWKKHQMPAWHLITADGQGITLVNIGVGPSNAKTICDHLAVLRPDVWLMIGHC
                     GGLRESQAIGDYVLAHAYLRDDHVLDAVLPPDIPIPSIAEVQRALYDATKLVSGRPGE
                     EVKQRLRTGTVVTTDDRNWELRYSASALRFNLSRAVAIDMESATIAAQGYRFRVPYGT
                     LLC"
BASE COUNT          287 a          312 c          276 g          310 t
ORIGIN      
        1 gccattggca aatatattac aattggcgaa ttacctgatg aaaacgcccg taaacaaggt
       61 ctttttgtct atccatcact gaccgtaacc tgggacggta gcacaacaaa tccccccaaa
      121 acgcgcgcat ttggtcgttt tactcacgca ggcagctaca ccaccacgat tactcgccct
      181 actctctttc gttcgtatct gaatgagcaa ctaacgttgc tttatcagga ttatggtgcg
      241 catatctctg tgcaaccctc gcaacatgaa atcccttatc cttatgtcat cgatggctct
      301 gaattgacac ttgatcgctc aatgagcgct gatttaactc gctacttccc gacaacagaa
      361 ctggcgcaaa tcggcgatga aactgcagac ggcatttatc atccaacgga attctccccg
      421 ctatcgcatt ttgatgcgcg ccgcgtcgat ttttccctcg cacggttgcg ccattatacc
      481 ggtacgccag tagaacattt tcaaccgttc gtcttgttta ccaactacac acgttatgtg
      541 gatgaattcg ttcgttgggg atgcagccag atcctcgatc ctgatagtcc ctacattgcc
      601 ctttcttgtg ctggcgggaa ctggatcact gccgaaaccg aagcgccaga agaagccatt
      661 tccgaccttg catggaaaaa acaccagatg ccagcatggc atttaattac cgctgatggt
      721 cagggtatta ctctggtgaa tattggcgtg ggaccgtcca atgctaaaac catctgcgat
      781 catctggcag tgctacgccc ggatgtctgg ttgatgattg gtcactgtgg cggattacgt
      841 gaaagccagg ccattggaga ttatgtcctt gcacacgctt atttacgcga tgaccacgtt
      901 cttgatgcgg ttctgccgcc cgatatacct attccgagca ttgctgaagt gcaacgtgcg
      961 ctttatgacg caaccaagtt agtcagcggc aggcctggtg aggaagtcaa acagcggctg
     1021 cgtacaggta ctgtggtaac cacagatgac aggaactggg agttacgtta ctcagcttct
     1081 gcacttcgtt ttaacttaag ccgggccgta gcaattgata tggaaagtgc aaccattgcc
     1141 gcgcaaggat atcgtttccg cgtgccatac gggacactac tgtgt
//
LOCUS       DAETPZ010000283         1159 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.289, whole genome shotgun sequence.
ACCESSION   DAETPZ010000283 DAETPZ010000000
VERSION     DAETPZ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.289"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..1126
                     /gene="pdeF"
                     /locus_tag="K0W59_004414"
                     /pseudo
     CDS             1..1126
                     /gene="pdeF"
                     /locus_tag="K0W59_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
BASE COUNT          255 a          261 c          260 g          383 t
ORIGIN      
        1 caacttatat aaaaatacgt gataaatggt ggggcttccg ctgttcctgc cttctttaat
       61 cttgcccatt ttcgcccaca ttaatacttt cgcgcatatt tcttccggtg aggtttttct
      121 cttttatctg cctttggcac tgatgatcag catgatgatg tttttcagct gggcggcatt
      181 gccagggatc accttaggga tttttgtccg caaatatgca gagctgggtt tttacgaaac
      241 gctctcatta acggctaatt ttattatcat tatcattctc tgttggggcg gttacagggt
      301 ctttactccc cggcgtaaca acgtttcaca tggtgatacc cgtttaattt cccagcgtat
      361 attctggcag atagtgtttc ctgcaacgct gtttctgata cttttccagt ttgctgcatt
      421 tgtaggatta ctggcgagca gagaaaatct ggtcggtgtc atgcctttta acctcgggac
      481 tttaatcaat tatcaggcct tactggtggg taatctgatc ggtgtcccgc tgtgctactt
      541 catcattcgg gtagtgcgaa atccatttta tttacgtagc tattattcgc aattaaaaca
      601 gcaggttgat gccaaagtca ccaaaaaaga gttcgcgctc tggctactgg cattaggtgc
      661 tttattgttg ctgttatgca tgccgttaaa tgaaaaaagc acaattttta gcaccaatta
      721 taccttgtca ttattgctgc ccctgatgat gtggggagca atgcgctatg gttataagct
      781 gatttcgctg ctctgggcgg tcgtgttgat gatcagcatc cacagctatc aaaattacat
      841 tcccatttat cctggctata ccacgcagct gaccataacc tcctccagtt atctggtatt
      901 ctcttttatt gtcaattata tggctgtact ggcaactcgt cagcgagcgg tagtcagacg
      961 cattcagcgg cttgcgtatg tggacccggt ggttcatctg ccaaatgttc gcgccctgaa
     1021 tcgcgcgttg cgtgatgccc tctggtctgc gctttgttat ttacgcatcc ctggcatgga
     1081 aatgctggtt aagaactatg gcatcatgct gcggattcaa tatgaggtgt actggcaata
     1141 gcggacacta ccatttgtt
//
LOCUS       DAETPZ010000284         1126 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.291, whole genome shotgun sequence.
ACCESSION   DAETPZ010000284 DAETPZ010000000
VERSION     DAETPZ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.291"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            252..1007
                     /gene="mlaA"
                     /locus_tag="K0W59_004415"
     CDS             252..1007
                     /gene="mlaA"
                     /locus_tag="K0W59_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HBI3025101.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          276 a          260 c          293 g          297 t
ORIGIN      
        1 gcttttttgc tcgcaactgg cgggagttac tctgaaaatg tagaaaaggt tgcgtttgcc
       61 tttttctgtt tctatagaat caagtagcct acagggcggc gattaccagg ctatgatcaa
      121 atcagcaaat cagggcgtct ggacatcagt tgacgtgctg ttacaatcgc ccacacctaa
      181 acaggcggat acggtatcgt tccgtcatgg atggcaaact gcataagcca taaaaaaaca
      241 gggagacatt tatgaagctt cgcctgtcgg cgcttgctct gggaactacg cttctggtgg
      301 ggtgtgcgag ttccggtaca gatcagcaag ggcgttctga cccgttagaa gggttcaacc
      361 gcaccatgta caacttcaac ttcaacgtat tagacccgta tattgttcga ccggtcgctg
      421 tcgcctggcg tgattatgtt ccgcaaccgg cgcgtaatgg tttgagcaac tttactggca
      481 accttgaaga acctgcggtg atggttaact acttcttgca gggcgaccct tatcagggga
      541 tggtccactt tacccgcttt ttcctgaaca ccattttggg gatgggcggc tttattgatg
      601 ttgcagggat ggcgaacccg aaactgcaac ggactgaacc tcaccgcttc ggtagtacgc
      661 ttggtcatta tggcgtgggt tatgggcctt acgttcagtt accgttctac ggtagcttca
      721 cgctgcgtga tgacggtggt gatatggcgg atggtcttta cccggttctt tcctggctga
      781 cctggccgat gtctgtgggt aaatggacgc ttgaagggat cgaaacccgc gctcagctgc
      841 tggattccga tggtctgctg cgtcagtcgt ccgatcctta tattatggtg cgcgaagcgt
      901 acttccagcg tcatgatttc atcgctaatg gcggcgaact caaaccgcag gaaaatccga
      961 acgcgcaagc gattcaggat gatttaaaag atatcgattc tgaataagaa acaaataaaa
     1021 aaggtgagtc gcaaaactca ccttttttgt tgttatccct caattaccac aagtaaaagg
     1081 agataaaccg acaatgaccc ggacgtaatg atgtaaaaat actgat
//
LOCUS       DAETPZ010000285         1110 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.292, whole genome shotgun sequence.
ACCESSION   DAETPZ010000285 DAETPZ010000000
VERSION     DAETPZ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.292"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..1019
                     /locus_tag="K0W59_004416"
                     /pseudo
     CDS             1..1019
                     /locus_tag="K0W59_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          316 a          270 c          255 g          269 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgagaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctagacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctatca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat attccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcatt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggttag
//
LOCUS       DAETPZ010000286         1108 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.293, whole genome shotgun sequence.
ACCESSION   DAETPZ010000286 DAETPZ010000000
VERSION     DAETPZ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.293"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..387)
                     /locus_tag="K0W59_004417"
     CDS             complement(<1..387)
                     /locus_tag="K0W59_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HBI3025102.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNV"
     gene            816..1097
                     /locus_tag="K0W59_004418"
     CDS             816..1097
                     /locus_tag="K0W59_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HBI3025103.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          307 a          250 c          252 g          299 t
ORIGIN      
        1 aacgttaggt tcacgcttat aatcggccgg tgcaatcgta tagccgttta caggcgctat
       61 cagcgatgag cataaaaaat ggttatcacg ggcatagatc aattcattaa tataaagata
      121 gccacgaata atattcaaca ttcgcttttg atgggctgga gtgcaaactt gcccctgata
      181 gcgttcagct gcatcgctca ctaaatctgc ctggcgaatg accagctcgg atttatccag
      241 tgccagttga gcaaaggttc ggagctgatt attcacttct gattttgccc aaaggaaggc
      301 aatccatagc gaaagaatga tgggaaagag aactaaaaag ataatgcccg gcaacgccag
      361 taattggtgt cgtgcacgat gactcattgc tatcaatttc ctgcctgttt atacccggcc
      421 attgcaaggt gaaaccctgg ctaccgacgt ctttagataa atcttacggt ataaatagca
      481 cttttgcctg atttgaaatt gatacctttt taatcgtcac ctggatgaaa ataattcatt
      541 gatattcaaa attaattttc tacaggaata cagaggcggc gggaagatat gcccttaatg
      601 ccactgaaaa accacataat ctgtacagca ataaaagtca cggcctaata tggctaacaa
      661 acaatcaatt gtgatatggt tcacaaaatt aatgaaacaa acagagtgtt tcatttttgt
      721 gttctgcaag ccccgcgata gccagagtgt accacgcctc ccgtgaacaa cgccgcgctg
      781 tccagggcat cggctctttt cagaggaatt gtttgatggc taccctcacc actggcgtgg
      841 ttcttcttcg ctggcaactt cttagtgccg taatgatgtt tctggccagc acactcaaca
      901 tccgttttcg tcggtctgat tatgtcgggc ttgcggtgat cagcagcggt ctgggcgttg
      961 tttccgcctg ttggttcgca atggggttgc ttggcatcac aatggcggac atcaccgcta
     1021 tctggcacaa catcgagtcg gtgatgatag aagagatgaa tcagacaccg ccacaatggc
     1081 caatgatttt gacctgatac gtagaagc
//
LOCUS       DAETPZ010000287         1107 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.294, whole genome shotgun sequence.
ACCESSION   DAETPZ010000287 DAETPZ010000000
VERSION     DAETPZ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.294"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="K0W59_004419"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="K0W59_004419"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HBI3025104.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMKALLEAKKQGLTREIGISNFTIPLMEKAIAAVGTENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDHLVSPEGLAPEWD"
     gene            complement(986..1062)
                     /locus_tag="K0W59_004420"
     tRNA            complement(986..1062)
                     /locus_tag="K0W59_004420"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1026..1028),aa:Asp,seq:gtc)
BASE COUNT          287 a          258 c          251 g          311 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggt
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag tgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctt cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ataagtggcg gaacggacgg gactcgaacc cgcgaccccc
     1021 tgcgtgacag gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac
     1081 caccggtttt atgaccggct tactgct
//
LOCUS       DAETPZ010000288         1088 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.295, whole genome shotgun sequence.
ACCESSION   DAETPZ010000288 DAETPZ010000000
VERSION     DAETPZ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.295"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(184..1053)
                     /locus_tag="K0W59_004421"
     CDS             complement(184..1053)
                     /locus_tag="K0W59_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HBI3025105.1"
                     /translation="MPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDDPFGAL
                     MQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETTDLPCW
                     RHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDLWQDEHLLRLCLR
                     QALQLADVVKLSEEEWRLISGKTQNDQDICALAKEYEIAMLLVTKGAEGVVVCYRGQV
                     HHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQRCGALAVTAK
                     GAMTALPCRQELE"
BASE COUNT          273 a          317 c          231 g          267 t
ORIGIN      
        1 acctgtgttg ttctgaggtg agcatatcac ctctgttcag gtggccaaat tcagtgtgcc
       61 acttcaatgc aaaacgatag tacgcatttc tgaatggaat attcagtgcc ataattacct
      121 gcctgtcgtt aaaaaattca cgtcctattt agagataaga gcgacttcgc cgtttacttc
      181 tcactattcc agttcttgtc gacatggcag cgctgtcatt gcccctttcg ccgttactgc
      241 aagcgctccg caacgttgag cgagatcgat aattcgtcgc atttctctct catctgtaga
      301 taatcccgta gaggacagac ctgtgagtaa cccggcaacg aacgcatctc ccgcccccgt
      361 gctatcgaca caattcacag acattccagc aaaatggtga acttgtcctc gataacagac
      421 caccacccct tctgcacctt tagtcaccaa cagcatggcg atctcatact cttttgccag
      481 ggcgcatata tcctgatcgt tctgtgtttt tccactgata agtcgccatt cttcttccga
      541 gagcttgacg acatccgcca gttgtagcgc ctgccgcaaa cacaagcgga gcaaatgctc
      601 gtcttgccat agatcttcac gaatattagg atcgaagctg acaaaacctc cggcatgccg
      661 gatcgccgtc atcgcagtaa atgcgctggt acgcgaaggc tcggcagaca acgcaattga
      721 acagagatgt aaccattcgc catgtcgcca gcagggcaag tctgtcgtct ctaaaaaaag
      781 atcggcactg gggcggacca taaacgtaaa tgaacgttcc ccttgatcgt tcagatcgac
      841 aagcaccgtg gatgtccggt gccattcatc ttgcttcaga tacgtgatat cgactccctc
      901 agttagcagc gttctttgca ttaacgcccc aaaaggatca tcaccgaccc gacctataaa
      961 cccacttatt ccgcctaatc tggcgattcc caccgcaacg ttagctggcg cgccgccagg
     1021 acaaggcagt aggcgcccgt ctgattctgg caagagatct acgaccgcat cccctaaaac
     1081 ccatactt
//
LOCUS       DAETPZ010000289         1078 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.296, whole genome shotgun sequence.
ACCESSION   DAETPZ010000289 DAETPZ010000000
VERSION     DAETPZ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.296"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>1078
                     /locus_tag="K0W59_004422"
     CDS             join(<1..238,238..>1078)
                     /locus_tag="K0W59_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HBI3025106.1"
                     /translation="KKPRKQHSPEFRSEALKLAERIGVTAAARELSLYESQLYNWRSK
                     QQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKYVFIEKHQA
                     EFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSKQRYGAPRL
                     TDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLLEQDFYASG
                     PNQKWPGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDALQMALWRRK
                     RPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVECIH
                     GEHFISREIMRATVFNYIECDYNRWR"
BASE COUNT          267 a          280 c          306 g          225 t
ORIGIN      
        1 aaaaaacccc gtaaacagca ttcgcctgaa tttcgcagtg aagccctgaa gcttgctgaa
       61 cgcatcggtg ttactgccgc agcccgtgaa ctcagcctgt atgaatcaca gctctacaac
      121 tggcgcagta aacagcaaaa tcagcagacg tcttctgaac gtgaactgga gatgtctacc
      181 gagattgcac gtctcaaacg ccagctggca gaacgggatg aagagctggc tatcctccaa
      241 aaggccgcga catacttcgc gaagcgcctg aaatgaagta tgtctttatt gaaaaacatc
      301 aggctgagtt cagcatcaaa gcaatgtgcc gcgtgctccg ggtggcccgc agcggctggt
      361 atacgtggtg tcagcggcgg acaaggataa gcacgcgtca gcagttccgc caacactgcg
      421 acagcgttgt cctcgcggct tttacccggt caaaacagcg ttacggtgcc ccacgcctga
      481 cggatgaact gcgtgctcag ggttacccct ttaacgtaaa aaccgtggcg gcaagcctgc
      541 gccgtcaggg actgagggca aaggcctccc ggaagttcag cccggtcagc taccgcgcac
      601 acggcctgcc tgtgtcagaa aatctgttgg agcaggattt ttacgccagt ggcccgaacc
      661 agaagtggcc aggagacatc acgtacttac gtacagatga aggctggctg tatctggcag
      721 tggtcattga cctgtggtca cgtgccgtta ttggctggtc aatgtcgcca cgcatgacgg
      781 cgcaactggc ctgcgatgcc ctgcagatgg cgctgtggcg gcgtaagagg ccccggaacg
      841 ttatcgttca cacggaccgt ggaggccagt actgttcagc agattatcag gcgcaactga
      901 aacggcataa tctgcgtgga agtatgagcg caaaaggttg ctgctacgat aatgcctgcg
      961 tggaaagctt ctttcattcg ctgaaagtgg aatgtatcca tggagaacac tttatcagcc
     1021 gggaaataat gcgggcaacg gtgtttaatt atatcgaatg tgattacaat cggtggcg
//
LOCUS       DAETPZ010000290         1071 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.297, whole genome shotgun sequence.
ACCESSION   DAETPZ010000290 DAETPZ010000000
VERSION     DAETPZ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.297"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..767
                     /locus_tag="K0W59_004423"
     CDS             <1..767
                     /locus_tag="K0W59_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBI3025107.1"
                     /translation="FATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRHQ
                     QTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNRRLSESVPCMPHKKAG"
     gene            788..1039
                     /locus_tag="K0W59_004424"
                     /pseudo
     CDS             788..1039
                     /locus_tag="K0W59_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
BASE COUNT          346 a          239 c          244 g          242 t
ORIGIN      
        1 aatttgcaac agataatggt tatacatcag gaaatccatt taacggtctg acaccattaa
       61 aaaagtccaa gatagaacca gatcccctca cccgtgacga atttattcgt tttattgagg
      121 cttgccgtca tcaacaaaca aaaaacctgt ggattatcgc tgtatacacg ggtattcgtc
      181 acggggagct ggtatcactg gcctgggaag atatagacct taaagcgagg actataacca
      241 tccgccggaa ttatacaaaa ctaggcgaat tcactccacc aaaaactgat gctggtaccg
      301 gaagaacgat tcatctagtt caaccagcta ttgatgctct taaaagccaa gcggaaatga
      361 ccatgcttgg aaaacagcat tctgtagagg taaagcagag ggaatatggg agaagtactg
      421 tgcataaatg cacttttgtt tttagtcctc aggtaataaa acagcggcag ttttccggac
      481 cgcactataa ggttgactcc atcagggagt catggacaag tatcttaaaa cgcgcaggtc
      541 tgagacacag aaaatcgtat caatccaggc atacctatgc atgctggtca cttgccgcag
      601 gagctaatcc tagttttatc gcaagccaga tgggccacac aaacgcacaa atggtattca
      661 atgtttacgg agcatggatg aaagacaaca atcacgaaca gatagaactt cttaacagaa
      721 gactatctga aagtgtccca tgcatgcccc ataagaaagc ggggtaaaat aaaaacttgc
      781 aaaatcaatt ggtttacctt taatccctgt cacgttacgc gcgtggcaga ggcgttacgg
      841 attgctgaaa ccgcaacgga cagacggcgg tcatcggctg ttcaacgatg ccgatattga
      901 ccggatccgc gagatcaaat gctggatcga caacggcgtg caggtcagca aagttaaaat
      961 actgctcagt aatgaaaatg ttgatgtgca gaacgtctgg cgcgatcagc aagaaacatt
     1021 actgacttac ctgcaaagcg tgaaccgccc cgggaatcct ggagactaaa c
//
LOCUS       DAETPZ010000291         1018 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.298, whole genome shotgun sequence.
ACCESSION   DAETPZ010000291 DAETPZ010000000
VERSION     DAETPZ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.298"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..275
                     /locus_tag="K0W59_004425"
     CDS             <1..275
                     /locus_tag="K0W59_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBI3025108.1"
                     /translation="IRRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSH
                     ADTQFKNVPSAFQVGYINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            277..>1018
                     /locus_tag="K0W59_004426"
     CDS             277..>1018
                     /locus_tag="K0W59_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3025109.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEEGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIAPEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTTV"
BASE COUNT          323 a          187 c          200 g          308 t
ORIGIN      
        1 tcatccgtag aaatgatggt tcgatccgct ttaacaataa gatgccttat cacgttacgc
       61 tgggttatat cggaacaaac ggaatcacaa tgttgccaca aacgcaaagt ttaatggtaa
      121 ccccttatag ccatgcagat acgcagttta aaaatgttcc gtcagctttt caggttggtt
      181 atatcaatga ttttggtgga ttaagttttt atgaaataaa ctgcccgacg gttaataact
      241 cttgtaatgt ttccgtagcc aaacgagata aataatatgc gctacttatt aattgtaatt
      301 atatttatta taggttttag tgcattacct gtatgggcaa tggactgcta tgctgaacat
      361 gaagagggaa ataccgtagt cattggttac gtaccaagaa tttctatccc cagtaatggt
      421 aaaaaaggtg ataagatctg gcaaagcagt gagtatttta tgaatgtctt ctgtaataat
      481 gcattacctg ccccatcacc tggagaagaa tacccatcag cctggactaa tataatgatg
      541 tttttaccag gcggtcagga cttttataat cagaactcct atatttttgg cgtaacatac
      601 aatggagttg attatgattc gactgcccct aatgctatcg cagcaccaga atgcattgat
      661 ataaaaggag cgggtacctt taataatcac tataaaaacc ccgctgtctg tagtggaggt
      721 cctgagcctc aattatcagt aacctttcca gctcgcgtac aactctatat taaattagca
      781 aaaaatgcca atagagttaa taaaaacctg gtgttacctg atgaatatat cgctccagag
      841 tttaaaggta tgagtggcgc aggagctata gaagtagaca aaaatttgac cttcaggatt
      901 cgtggattaa ataatattca tgtccttgac tgctttgtta atgtcgcact ggaaccagct
      961 gatggcgttg ttgactttgg taaaataaat tcccgaacaa ttaaaaatac caccgtta
//
LOCUS       DAETPZ010000292          999 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.299, whole genome shotgun sequence.
ACCESSION   DAETPZ010000292 DAETPZ010000000
VERSION     DAETPZ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.299"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            66..281
                     /locus_tag="K0W59_004427"
     CDS             66..281
                     /locus_tag="K0W59_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBI3025110.1"
                     /translation="MYQMEKISTGIAYGTSGGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            281..778
                     /gene="rrrD"
                     /locus_tag="K0W59_004428"
     CDS             281..778
                     /gene="rrrD"
                     /locus_tag="K0W59_004428"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HBI3025111.1"
                     /translation="MSPSLRKAVAVAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLVTVTRQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            775..>999
                     /locus_tag="K0W59_004429"
     CDS             775..>999
                     /locus_tag="K0W59_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBI3025112.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRAADALDAKYTTELANA"
BASE COUNT          261 a          227 c          271 g          240 t
ORIGIN      
        1 catcagagtt ttgctgatgg ccttttttct ttccgatagc acaggtctgt cggggggggc
       61 gggatatgta tcagatggaa aaaatatcaa caggcattgc ctacggcacc tccggcggca
      121 gtgccggcta ctggtttttg cagtggttgg atcaggtcag tccatcacag tgggctgcga
      181 ttggtgtgct gggaagtctg cttctggggc ttctgactta tctgacgaat ctgtatttca
      241 aaataagaga agataagcgt aaggctgcga gaggtgaata atgtcgccat cattacgcaa
      301 ggctgttgca gttgctattg gtggcggggc tgttgctata gcatctgtgt taatcactgg
      361 cccaggtggt aacgatggtc tggaaggtgt cagctacata ccatacaaag atatcattgg
      421 tgtatggact gtatgtcacg gacacaccgg aaaagacatc atgcctggta aaacgtatac
      481 cgaagcagaa tgcaaagctc tcctgaataa agaccttgtc actgtcacca gacaaattaa
      541 cccgtacatc aaagtcgata taccggaaac aacgcgcggc gctctttact cgttcgttta
      601 caacgtgggc gctggcaatt tcagaacatc gacgcttctt cgcaaaataa accagggcga
      661 tatcaaaggc gcatgtgacc agctacgtcg ctggacatat gctggcggta agcaatggaa
      721 agggctgatg acccgtcgtg agattgagcg tgaagtctgt ttgtgggggc agcaatgagc
      781 agagtcaccg cgattatctc cgctctggtt atctgcatca tcgtctgcct gtcatgggct
      841 gttaatcatt accgtgataa cgccattacc tacaaagagc agcgcgacaa aaacgcaaga
      901 gaactgaagc tggcgaacgc aaccattact gacatgcagc agcgccagcg tgctgctgat
      961 gcactcgatg ctaaatacac gacggagtta gccaatgcg
//
LOCUS       DAETPZ010000293          978 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.300, whole genome shotgun sequence.
ACCESSION   DAETPZ010000293 DAETPZ010000000
VERSION     DAETPZ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.300"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            88..978
                     /locus_tag="K0W59_004430"
                     /pseudo
     CDS             88..978
                     /locus_tag="K0W59_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
BASE COUNT          259 a          229 c          242 g          248 t
ORIGIN      
        1 agttgtttac tgtgcgctgt tgtccatgtg atattgtgta ccagatgtgt gccaaactat
       61 tatttctgag cgtaccaaat attatttatg gctataagcg acacaaaact gcgtactatt
      121 tatggtaaac catattcggg cccacaagaa gtggctgatg ccgatggcct cagcgtacga
      181 atttcaccga agggggtcat ccagttccag taccgctatc gctggcatgg caagcctaat
      241 cgacttgggc ttggtcgata cccatccctg tctttgaagg atgccagaca gatcactgct
      301 gacttgcgaa agctctattt ctcaggaacg gatccacgca cctattttga agagaaggtg
      361 gagaactcca tgacggtcgc ccagtgtctc gactactggt tcgacaacta cgtctctaca
      421 actctcagag aaaaggccta ggcactttat cgatcaacgg ttatgaagcg catgcatgac
      481 gccttcccta atcgtccggc atcttctatc acggttaagc aatgggttga cctgcttacc
      541 gaagaagaaa aagataatcc acgccgagca aggcaggtgc taagtcaact aagatcagca
      601 attagttggt gcatgcggcg tcagttgata gatagttgcg caattatgag catccagcca
      661 agggacttcg gctcccgcgc tgaggtaggg gatcgggtac tgtcgtatca cgaactggct
      721 aagatttggc ttgctattga aagaagccgt gcgtctacgt caaataagct ccttcatcag
      781 atgcttatgc tgtggggggc gaggctctca gagcttaggc tggcaacaaa gacagaattt
      841 gacctgctgg acaacgtatg gaccgtaccg aaagagcata gcaagatggg taatgttatc
      901 cgccgtccaa tcttcgaaca aattaagcct tttctcgaaa aggccatgac aatgtacaat
      961 gatgttcttt tccctgga
//
LOCUS       DAETPZ010000294          952 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.301, whole genome shotgun sequence.
ACCESSION   DAETPZ010000294 DAETPZ010000000
VERSION     DAETPZ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.301"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(33..578)
                     /locus_tag="K0W59_004431"
                     /pseudo
     CDS             complement(33..578)
                     /locus_tag="K0W59_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(644..>952)
                     /locus_tag="K0W59_004432"
     CDS             complement(644..>952)
                     /locus_tag="K0W59_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HBI3025113.1"
                     /translation="RFINTDKAPAYGRALALLKREGRCPSDVEHRQIKYRNNVIECDH
                     GKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPLGEMRLVSRVFEM
                     "
BASE COUNT          209 a          247 c          237 g          259 t
ORIGIN      
        1 gggtctgacg ctcagtggaa cgaaaactca cgttaagcaa cgttttctgc ctctgacgcc
       61 tcttttaatg gtctcagatg tcctttggtc accagttctg ccagcgtgaa ggaataatgg
      121 ccgagcatat tgatatgtcc gtggcaaagc ggggagaggc gtgcgatatc ttcatcattc
      181 agtgtttcac cctgcgcccg gagatgatcc agggctgcct gcatataaat agtgttccat
      241 aacacgacgg cgttagtgac cagccccagt gcgcccagtt gatcttcctg accgtcggta
      301 tatcgttttc ttatctcacc tttttgaccg tgacagatgg ctctggcaac ggcatggcga
      361 ctttctcccc gattaagctg ggtcagaatg cgccggcggt aatcttcatc atcaatataa
      421 ttaagcagat acagcgtttt gttgatgcgc cccacttcaa tgattgcctg agtcagtccg
      481 gaaggacgtt cacttttcag caatgaacgg accagcactg aagcctgtac tttgcccagc
      541 ttcagggagc cagcggtccg gatcatttcg tcccactgaa ggactatttt tggcactgtt
      601 gcaaagttag cgatgaggca gccttttgtc ttattcaaag gccttacatt tcaaaaactc
      661 tgcttaccag gcgcatttcg cccaggggat caccataata aaatgctgag gcctggcctt
      721 tgcgtagtgc acgcatcacc tcaatacctt tgatggtggc gtaagccgtc ttcatggatt
      781 taaatcccag cgtggcgccg attatccgtt tcagtttgcc atgatcgcat tcaatcacgt
      841 tgttccggta cttaatctgt cggtgttcaa cgtcagacgg gcaccggcct tcgcgtttga
      901 gcagagcaag cgcgcgacca taggcgggcg ctttatccgt gttgatgaat cg
//
LOCUS       DAETPZ010000295          926 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.302, whole genome shotgun sequence.
ACCESSION   DAETPZ010000295 DAETPZ010000000
VERSION     DAETPZ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.302"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>926
                     /locus_tag="K0W59_004433"
     CDS             <1..>926
                     /locus_tag="K0W59_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HBI3025114.1"
                     /translation="DIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQLDRLRRML
                     FGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSPPSENPIA
                     SKPESPGRKSSRKPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQLDIINTAF
                     KVIETIRPKLACSRCDVIVQAPLPPKPIERGYASAGLLARILVSKYMEHIPLYRQSEI
                     YARQGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKT
                     KTGRLWVYVRDDRNAGSSLPAAVWFAYSADRK"
BASE COUNT          253 a          233 c          249 g          191 t
ORIGIN      
        1 tgatattgag ctgcttaaag ccatgttgcg taagcaacag agtcggcttc gacagtatgc
       61 ctgtcaggtc gcgggctatg agcaggaaat tgaacggctg aaagcgcaac tcgacaggtt
      121 gcgtcgtatg ttgttcggcc agagttcaga gaaaaagcgt cataagcttg aaaatcagat
      181 ccgacaggca gaaaaacgac tgtcggaact ggaaaaccgg ctgaacacag ccagaaatct
      241 tctggaagat gcatcgtcag tcacagattc acctgacacc agtcccccgt cagaaaaccc
      301 gatcgccagt aagcctgaat ccccgggacg aaaatcttca cgaaaaccgc tgccggcaga
      361 acttccccgg gagacacatc gccttctgcc tgctgaaacc agttgcccgg cctgtggagg
      421 tgttctgaaa gaaatggggg aaacaatctc agagcaactg gatatcatta ataccgcctt
      481 taaagttatc gaaaccatac gtcccaaact ggcctgtagc cggtgtgatg tcatcgttca
      541 ggcaccactt ccccctaaac cgatcgaacg cggttatgcc agtgcagggt tacttgcacg
      601 gatcctggtc agcaaatata tggaacatat ccctttatat cgccagtcag aaatatacgc
      661 gcgacagggc gtggagctga gccgtaatac catggtgcgc tgggtatcag aaatggcaga
      721 caaactccgt cctctgtata tagcgctgaa tgactatgtt ctggaggcag gaaaggtgca
      781 cgcagatgac actccggtga aagtactggc cccggggaac ggaaagacga aaacgggtcg
      841 tctgtgggta tacgtcaggg atgatcgtaa tgcgggttca tccctgccgg cagccgtctg
      901 gttcgcgtat tcggcagatc gcaaag
//
LOCUS       DAETPZ010000296          908 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.303, whole genome shotgun sequence.
ACCESSION   DAETPZ010000296 DAETPZ010000000
VERSION     DAETPZ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.303"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>908
                     /locus_tag="K0W59_004434"
     CDS             <1..>908
                     /locus_tag="K0W59_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBI3025115.1"
                     /translation="TNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTL
                     WPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTC
                     GGLNKHGQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITTESQRRSLV
                     LKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLDHKT
                     GETATETLTQRELVARLKQHIPEKFFKMVRYFGFLASRVCGEKLPQVYRALGMDKPEP
                     VAKVCYAQMVKQFLSRDPFECVLCGGR"
BASE COUNT          207 a          226 c          298 g          177 t
ORIGIN      
        1 actaactcat gcggcagccg tgcctgcccg tcctgcggaa aaaaggccac agacctgtgg
       61 atagcgacac agctgaatcg tcttcctgac tgcgactggg tacacctggt cttcaccctg
      121 ccggacacgc tgtggccggt gttcgaaagc aaccggtggc tgctgaatga cgtgtgccgt
      181 ctggcggtgg agaatctgct gtatgccgcc cggaaacggg ggcaggaacc cggtatcttc
      241 tgcgccatcc acacgtatgg ccgtcgtctc aactggcatc cgcatgtaca tgtgtctgta
      301 acctgtggag gtctgaataa gcatggtcag tggaaaaagc tgagcttcct gaaagacgcg
      361 atgcgttcgc ggtggatgtg gaatatgcgg caacggcttc tgaaagcgtg gtcagagggg
      421 ctggcgatgc cggagtcgtt gtcacatatc acgacggaat cacagaggag aagcctggtg
      481 ctgaaagccg gaggaaaata ctggcatgtg tacatgtcga aaaaaacggc cggcgggagg
      541 aatacggcgc gctacctggg tcgttatctg aagaagccgc cgatagcggc ctcccggctg
      601 gcacattaca acggaggggc gagcctgagc ttccgttacc tggaccacaa aacgggagaa
      661 acggcgacgg aaacgctgac acagcgtgag ctggtcgcga ggctgaaaca gcacatcccg
      721 gagaagtttt ttaagatggt gaggtacttc gggttccttg ccagccgggt gtgtggagag
      781 aagctgccgc aggtgtaccg tgctctgggg atggataaac cggaaccagt ggcgaaagtg
      841 tgctatgcac aaatggtgaa acagttcctg agtcgtgacc cgttcgaatg cgtgctgtgt
      901 ggcggccg
//
LOCUS       DAETPZ010000297          894 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.304, whole genome shotgun sequence.
ACCESSION   DAETPZ010000297 DAETPZ010000000
VERSION     DAETPZ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.304"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(110..439)
                     /locus_tag="K0W59_004435"
     CDS             complement(110..439)
                     /locus_tag="K0W59_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HBI3025116.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(611..>894)
                     /locus_tag="K0W59_004436"
     CDS             complement(611..>894)
                     /locus_tag="K0W59_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3025117.1"
                     /translation="FVLLNAQKLRDTQHMMQQILLSLVHALYEGNPQPVFANTEKLND
                     AVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          217 a          207 c          199 g          271 t
ORIGIN      
        1 tgttgttaat ttcgattggt tctcagatga gaatgatgac ggtgatacac agataagaat
       61 ctatgagcaa tgagggttgc cgggcaaccc tcattgaata aaacgggaat tacttcgctt
      121 cgccgttttt catttcgccc atagctttca gctttttgga gatatcgcgg cgttctttgg
      181 agagctcggc atttttgatg atgtaatcgt caacgcgatc ttcatagtca cctttcatgc
      241 tggcgatgat gccctggatt gcttcaacgc tcatccctgg cttgatgtaa tcgctcaggt
      301 tgtccagcaa caggacgcgc ttctggttgt cacggatctt tttctcaacg tcctgaattt
      361 cacgttgcag tttgttctta cgacggaaca gacgaacaaa ttccagtacg tcctggaatg
      421 aaggcttggt agtttccatt tttatacccc tgataatgtg agagtcggat tcgtttaagc
      481 aaccgctatt cgttagggcc aacattactc atagctgccg cagatgacaa tgcttttatc
      541 ccttttcact atcataccct ttatccttgc tgaatcgaag cagcagcaag atgattctga
      601 agttcaggaa ttatttgcgc aaggcccggc aaatcagatt cgatagcaac tcaagctgat
      661 gtgccgtttc catgttcagc cacacataac catagattgg tgtttccacg actttcaggt
      721 catggtggtt atcgagcaac tgacgcagct cttccacagc atcgttcaat ttttccgtat
      781 tggcaaaaac cggctgcgga ttaccttcgt acagcgcatg aacaaggctc agcagtattt
      841 gctgcatcat gtgctgggta tcacgaagtt tttgcgcgtt caataacacg aaat
//
LOCUS       DAETPZ010000298          882 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.305, whole genome shotgun sequence.
ACCESSION   DAETPZ010000298 DAETPZ010000000
VERSION     DAETPZ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.305"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..692
                     /locus_tag="K0W59_004437"
     CDS             <1..692
                     /locus_tag="K0W59_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3025118.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLLGGLSCLTIPCFAWLAD
                     RFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFT
                     EMFGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAIYLLAGCLISAM
                     TALLMKDNQRS"
BASE COUNT          198 a          198 c          220 g          266 t
ORIGIN      
        1 tactgggagc attgtgggtg cgcaatggca tggaggagtc cgcggaattt gaacaacagc
       61 aacataatca agcggccgcg aaaaaacgca tcccggttat cgaagctctg ttacgacatc
      121 ccggtgcttt cctgaagatt attgcactac gactgtgcga gttgctgacg atgtacattg
      181 ttactgcctt tgcacttaat tattcaaccc agaatatggg gttaccgcgc gaacttttcc
      241 ttaatattgg tttgctgtta ggtggattaa gctgcctgac aattccctgt tttgcctggc
      301 ttgccgatcg ttttggtcgg cgcagggttt atatcacagg cgcgttgatc ggaacgttga
      361 gcgcatttcc tttctttatg gcgcttgaag cacaatctat tttctggata gttttcttct
      421 ccataatgct ggcaaacatt gcgcatgaca tggtggtgtg tgtgcaacaa ccgatgttta
      481 ccgaaatgtt tggtgccagt tatcgctaca gtggtgctgg agtcggttat caggttgcca
      541 gtgtggttgg cggtggattt acacctttta ttgccgctgc actcatcact tactttgccg
      601 ggaactggca tagcatcgcc atttatttgc tggctggatg cctgatttct gcaatgaccg
      661 ctttattgat gaaagacaat caacgctcgt gatagcctgc cgaagatcat ccgatcttcg
      721 ccttatactt ttgtttcaca ttcctgtgac atactatcgg atgtgcggta attgtatgga
      781 acaggagaca cacatgaata ataagggctc cggtctgacc ccagctcagg cactggataa
      841 actcgacgcg ctgtatgagc aatctgtagt tgctttacgc aa
//
LOCUS       DAETPZ010000299          881 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.306, whole genome shotgun sequence.
ACCESSION   DAETPZ010000299 DAETPZ010000000
VERSION     DAETPZ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.306"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..452)
                     /locus_tag="K0W59_004438"
     rRNA            complement(<1..452)
                     /locus_tag="K0W59_004438"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          197 a          244 c          191 g          249 t
ORIGIN      
        1 ttcctccccg ctgaaagtac tttacaaccc gaaggccttc ttcatacacg cggcatggct
       61 gcatcaggct tgcgcccatt gtgcaatatt ccccactgct gcctcccgta ggagtctgga
      121 ccgtgtctca gttccagtgt ggctggtcat cctctcagac cagctaggga tcgtcgccta
      181 ggtgagccgt taccccacct actagctaat cccatctggg cacatccgat agcaagaggc
      241 ccgaaggtcc ccctctttgg tcttgcgacg ttatgcggta ttagctaccg tttccagtag
      301 ttatccccct ccatcgggca gtttcccaga cattactcac ccgtccgcca ctcgtcagcg
      361 aaacagcaag ctgtttcctg ttaccgttcg acttgcatgt gttaggcctg ccgccagcgt
      421 tcaatctgag ccatgatcaa actcttcaat ttaaaagttt gatgctcaaa gaattaaact
      481 tcgtaatgaa ttacgtgttc actcttgaga cttggtattc atttatcgtc ttgcgacgtt
      541 aagaatccgt atcttcgagt gcccacacag attgtctgat aaattgttaa agagcagtgc
      601 cgcttcgctt tttctcagcg gcgcgaggtg tgcataatac gctttcccgc tacagagtca
      661 agcatttatt tttgctttct ctgccggaat tctcaggaga accccgctga cccggcggcg
      721 tgtttgccgt tgttccgtgt cagtggtggc gcattatagg gagttattcc ggcctgacaa
      781 gcgaaaaata taaaaacttt atcgttcgct cacttttcag gcaaaacatc ttaaatatag
      841 tcttttccgt ctaacttata gacaaaaacg agccccgaag g
//
LOCUS       DAETPZ010000300          839 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.307, whole genome shotgun sequence.
ACCESSION   DAETPZ010000300 DAETPZ010000000
VERSION     DAETPZ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.307"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            145..>839
                     /locus_tag="K0W59_004439"
     CDS             145..>839
                     /locus_tag="K0W59_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HBI3025119.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLHELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLHPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSYVGG"
BASE COUNT          223 a          216 c          226 g          174 t
ORIGIN      
        1 cgactctttt atcaacgctt acgtaattaa gggacacagc ctaaatagct gcgcggaata
       61 gtagatccct ttgagggaac ttagcccgga ttgtgcgatc tgatcaatcg ccaaatcaaa
      121 acaaatcacc aaccggactg agcaatgccg atcatagcac caatttcccg tgacgaacga
      181 cgcctgatgc agaaagccat ccataaaaca cacgataaaa attatgcccg cagactgact
      241 gccatgctga tgctgcaccg gggcgaccgt gtcagcgacg ttgccagaac gctctgctgc
      301 gcccgttcct cggttggacg ctggattaac tggttcacgc agtcgggtgt tgagggactg
      361 aaatcattac ctgccgggcg tgcccgtcgc tggccgtttg agcatatctg cacactgtta
      421 catgagctgg taaaacattc tcccggcgac tttggctacc agcgttcacg ctggagtaca
      481 gaactgctgg caataaaaat caatgagata accggttgcc agttaaatgc cggaaccgtt
      541 cgccgctggt tgccgtctgc ggggattgtg tggcgaaggg ctgcgccaac tctgcgtatc
      601 cgtgacccgc ataaagatga aaagatggca gcaatccata aagcactgga cgaatgcagc
      661 gcagagcatc cggtctttta tgaagatgaa gtggatatcc atcttcatcc caaaatcggt
      721 gcggactggc aactgcgcgg acagcaaaaa cgggtggtca cgccgggaca gaatgaaaaa
      781 tattatctgg ccggagcgct gcacagcggg acaggtaaag tcagctatgt gggcggcaa
//
LOCUS       DAETPZ010000301          820 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.308, whole genome shotgun sequence.
ACCESSION   DAETPZ010000301 DAETPZ010000000
VERSION     DAETPZ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.308"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            31..318
                     /locus_tag="K0W59_004440"
     CDS             31..318
                     /locus_tag="K0W59_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBI3025120.1"
                     /translation="MFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGHEMYYSPGSNT
                     VSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNVASI"
     gene            321..>820
                     /locus_tag="K0W59_004441"
     CDS             321..>820
                     /locus_tag="K0W59_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBI3025121.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSS"
BASE COUNT          243 a          175 c          216 g          186 t
ORIGIN      
        1 ttcagccaga gaaacccgga taacagcata gtgttcactg ttaacggaca ggtaattccg
       61 ttaaattacg gaaacttcga tgcccgctat aaatatcgaa cagagggggt acaggatgta
      121 cggtatggcc atgaaatgta ttacagcccc ggcagtaaca ccgtttcgtg gagattttgc
      181 gcaccttcgg gacacgggct gtcagggatg gcgatatcgg ataccggccg taactcagcg
      241 gataacgttg acggtgtgta ttaccgacca ctgcaaaaac tgattaatgg cacctggtat
      301 aacgtagcga gtatttaaca atgttgcatt taaaaaatat tactgcgggt aatccgaaaa
      361 cagcagaaca atatcagatg acaaaacgat attcggtcac ctggcttttt tcagaagacg
      421 gaaaaaactg gtatgaagag ctgaagaatt tcgccagcga cacaataaaa atagcttaca
      481 ccggagatgg tcgcgtggtg tgggtcggta aggatgtgac aggcatcgag ccacgcaatg
      541 ccagcgttat cgaagttccg gatattaccg ctaaccgacg gattaccgcg ccgggttact
      601 ggttttaccg caatgatgaa tttgtctttg actacagact caaagcggaa gatgagcgtg
      661 atgcccttct ggctcaggtc agtgcccgga caggggaatg ggaagaagac ctgctgctgg
      721 ggttaatcag cgacgaagat aaagaaaagc tgaaagcctg tcgtatttac gcgaaatcgc
      781 tgcaggcgat ggatttcagc accatcactg ataaatcctc
//
LOCUS       DAETPZ010000302          791 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.309, whole genome shotgun sequence.
ACCESSION   DAETPZ010000302 DAETPZ010000000
VERSION     DAETPZ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.309"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..355
                     /locus_tag="K0W59_004442"
                     /pseudo
     CDS             1..355
                     /locus_tag="K0W59_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            368..733
                     /locus_tag="K0W59_004443"
                     /pseudo
     CDS             368..733
                     /locus_tag="K0W59_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
BASE COUNT          223 a          168 c          195 g          205 t
ORIGIN      
        1 ttccaacgac cggtagcaca agtccagccg tcccttttca gattacgttt agtgaatgca
       61 gcaaagggac aacgggggtt cgggttgcat ttaacggtat tgaggatgca gaaaataata
      121 ctctgttgaa actggatgag ggaagcaata cggcctccgg tttgggtaag aaatactgga
      181 cggaaatatg cgtccggtga aactgaatga ccttcatgcc gggatgcagt ggatcccact
      241 ggtaccagaa cagaacaata ttttgcctta ctccgctcgt ctgaagtcaa ctcagaagtc
      301 cgtcaatccg ggactggtga gggcttcggc aacctttacc cttgaatttc aataatcaga
      361 gtcagatatg aaaagacttc acaagaggtt cctgttagct acgttttgcg cgttattcac
      421 agcaactctc caggccgccg atgtcactat cactgttaat ggtcgggtag tcgctaaacc
      481 ctgcactatt caaaccaaag aagctaacgt taatctcggg gatctttata cgcgcaatct
      541 gcaacaacct ggttctgcat ctggctggca caatattact ttgtcattaa ccgattgtcc
      601 ggttgaaaca agtgcagtga cggcaatcgt gacaggttca actgacaata cgggttatta
      661 caaaaatgaa ggtactgccg aaaatattca gatagagctg agggatgacc aggatgctgc
      721 gttaaaaaat ggagggtaat gctgccaact tactgattta gtgtatgatg gtgattttaa
      781 ggtgcttgcg t
//
LOCUS       DAETPZ010000303          772 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.310, whole genome shotgun sequence.
ACCESSION   DAETPZ010000303 DAETPZ010000000
VERSION     DAETPZ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.310"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..156
                     /locus_tag="K0W59_004444"
     CDS             <1..156
                     /locus_tag="K0W59_004444"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="catalyzes the formation of thymidine 5'-phosphate
                     from thymidine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBI3025122.1"
                     /translation="LRLDQTGRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAI
                     QERHRHD"
     gene            complement(442..>772)
                     /locus_tag="K0W59_004445"
     CDS             complement(442..>772)
                     /locus_tag="K0W59_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBI3025123.1"
                     /translation="KKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSES
                     CGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRGRAFPRVVKERPWKYPTAP
                     KKSQSVA"
BASE COUNT          191 a          192 c          170 g          219 t
ORIGIN      
        1 ctgcgtcttg atcaaacagg cagaccttat aacgaaggtg agcaggtggt aattggtggt
       61 aatgaacgat acgtttctgt atgccgtaaa cactataaag aggcgttaca agtcggctca
      121 ttaacggcta ttcaggaaag gcatcgccac gattaataag aatttcttta ctgacagggt
      181 gagcagggca cttttatcct gtcagttcgt tttacgcact tcttccgggc tatataccct
      241 tctcggcagt tttttaacgc cgctatacgc ctcacagggc tcttaagcac cgacgttgac
      301 ttgtgacctg taaagtacaa tatccctgtg tttaggcgtt atacatcgtc gcaaatatga
      361 tgaaggctaa tgctgtcggt ttatggaaaa gttgctttgg gtaaacaaaa aaacggcccc
      421 agaaggggta atgccagtca gttaagcaac tgactggctc tttttcgggg ctgtggggta
      481 tttccagggc ctctccttta ccactctcgg gaaggccctt ccccttctcg tcggtaattt
      541 cacaagttgt cccatacttg caagatcgcg cattagctcc ggtatacgtc ccggtgaagc
      601 gccctgcaat gtcatcagca ttctcatcac cattccgcat gattctgaga aactcagttg
      661 attcggccag taacctttca gatgttccgc cattttaatc atctgatatc tcaccagatt
      721 ataagccagt aagacacccc acagctcttg ctccacaagc tccggctttt ta
//
LOCUS       DAETPZ010000304          759 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.311, whole genome shotgun sequence.
ACCESSION   DAETPZ010000304 DAETPZ010000000
VERSION     DAETPZ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.311"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..317
                     /locus_tag="K0W59_004446"
     CDS             <1..317
                     /locus_tag="K0W59_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HBI3025124.1"
                     /translation="KKADIALYLLSKFNFTKQDVAEMEKMNNNRYCNLYDVEYLLSKD
                     GANYKVLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRF
                     EI"
     gene            655..>759
                     /locus_tag="K0W59_004447"
     CDS             655..>759
                     /locus_tag="K0W59_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3025125.1"
                     /translation="MSRKNQRYSTEFKAEAVKTVPENQLSISEGASRLS"
BASE COUNT          254 a          135 c          160 g          210 t
ORIGIN      
        1 ataaaaaagc tgatatagca ctttatttac tatctaaatt taattttaca aaacaagatg
       61 tcgcagagat ggagaaaatg aacaacaata gatattgtaa tctgtatgat gttgaatact
      121 tacttagtaa agatggtgcc aactataaag tgctagaata ttttatcaat aatggattgg
      181 ttgatgtaaa caaaaaattt caaaaagcaa atagtgggga cactatgctt gataatgcaa
      241 tgaaaagcaa agattcaaaa atgattgatt ttttattaaa aaatggagca atattaggca
      301 aacgatttga gatatgacct aaaagctcgg ccaaataata atgtcgtcga ttatcgaagt
      361 catcatgagt gattctgtcc gcacagtgca gcatcagcat gacagtgaat tgtctggcgt
      421 ggtccattta atgagatgga gatggtccct ctttcctaaa tagctgtgcc taataccgct
      481 acacttttgc cagcccatgt ttgcctccgg ggaatgggct gacggttcct tcaaaatcca
      541 ttgcacgcag ttatgtcgga gcgggaactt gactcctgtc tttacgggag tgaggtagcc
      601 tgagtttaac ggacactcct tcctgaaata gaatggcatc agaaggagcc aataatgagc
      661 agaaaaaacc aacgttactc tacggagttt aaagccgaag ccgtcaaaac ggttcctgaa
      721 aatcagcttt cgatcagtga aggcgcttcc cgattatct
//
LOCUS       DAETPZ010000305          756 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.312, whole genome shotgun sequence.
ACCESSION   DAETPZ010000305 DAETPZ010000000
VERSION     DAETPZ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.312"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..91
                     /gene="yjdI"
                     /locus_tag="K0W59_004448"
     CDS             <1..91
                     /gene="yjdI"
                     /locus_tag="K0W59_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HBI3025126.1"
                     /translation="WVMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            103..375
                     /locus_tag="K0W59_004449"
     CDS             103..375
                     /locus_tag="K0W59_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBI3025127.1"
                     /translation="MEICEGHNNFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            602..>756
                     /gene="ghoS"
                     /locus_tag="K0W59_004450"
     CDS             602..>756
                     /gene="ghoS"
                     /locus_tag="K0W59_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBI3025128.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLN"
BASE COUNT          236 a          128 c          186 g          206 t
ORIGIN      
        1 gtgggtcatg ccggatgaag tcgacgtcgc cactgtggtt aaagtgattg atacgtgccc
       61 gagcggcgcg ctgaaatacc gtcataaata agcgagggta aaatggaaat atgcgaaggc
      121 cacaataatt tttacattaa tgacgaacaa ggcaagcaaa tcgctgaaat tgtctttgtg
      181 ccgaccggag agaatttagc gattatcgaa cataccgatg tcgatgaaag cctgaaaggg
      241 caagggattg gtaaacagct ggttgcgaaa gtcgtggaaa aaatgcgtcg ggaaaaacga
      301 aaaattatcc cattatgtcc atttgcgaaa cacgaatttg ataaaacgcg ggagtatgat
      361 gatattcgca gttgatggga gagtacagag tcacgatatt tttcttgctc tccgcggtgt
      421 gatgcaggag agcatctgaa aggtaggggg atgcacaaag aatgggcaga gagcgcgttt
      481 ttttgtccca agtcatcccc tttactgagc aaaaaaaaga atatctccta tatgagaatc
      541 atcaatcggg gttaataagt tttgcgtccc cagagcgttt aatattgata ggagtcatat
      601 tatggaaggt aaaaacaagt tcaatactta tgttgtttct tttgattatc catcatctta
      661 ttcctcagtg ttcttaagat taagatcatt gatgtatgat atgaatttct cctctatcgt
      721 ggctgatgaa tatgggatac cacgacaatt gaatga
//
LOCUS       DAETPZ010000306          731 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.313, whole genome shotgun sequence.
ACCESSION   DAETPZ010000306 DAETPZ010000000
VERSION     DAETPZ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.313"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..580)
                     /locus_tag="K0W59_004451"
                     /pseudo
     CDS             complement(1..580)
                     /locus_tag="K0W59_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            complement(531..>731)
                     /locus_tag="K0W59_004452"
     CDS             complement(531..>731)
                     /locus_tag="K0W59_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3025129.1"
                     /translation="QSYRGSRLGPVGISVPSTPTGTYIVSPQFFITGCSEHSLPGSYC
                     ALSGVPDAHVSGAMPGLFIRTS"
BASE COUNT          150 a          212 c          180 g          189 t
ORIGIN      
        1 gctcagctcc tctttcattt cacgctgacg gcgataaatc tcatcgttgc gctcaacaat
       61 ggcctgtatc atcgctgtgg ccagttcctc aagttcctgc gtcgtcagct gcacctgctg
      121 attatccgca tcactccacg tcatatttgt ttgtgccgcg acagattttg ccagcatgac
      181 caccggggac aggcggccca gtgagttggg gccagcattc cagatacgac cgttccattc
      241 aaacgtgaac ggcttcgcct cctgttctgc gcgccatgct tcaatttcct gacgtctggc
      301 ctctctggcc gcttccagca tttctggtgt cacagtgaat ggggctatct caccccattt
      361 gccactttgc agttcctgcc agattcgctg acccgtcggt gcgacatcat cagcggtggc
      421 tgtgtagggg actgcctggt ccctgtcgtc aaaaaaaaac gtcacagtct actgcgccac
      481 tttcggtata acggggatta atgatttttt taatttccac ggtgcattcc tcacgatgtg
      541 cgaataaaaa gcccgggcat tgcgccagag acatgtgcat ccggcacccc ggacagggcg
      601 caatatgacc ccggtaatga atgctctgaa catcccgtaa tgaaaaattg tggggatact
      661 atatacgttc cggtgggagt actgggcact gaaatcccca ccggtcccag tcgtgagcct
      721 ctgtatgact g
//
LOCUS       DAETPZ010000307          729 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.314, whole genome shotgun sequence.
ACCESSION   DAETPZ010000307 DAETPZ010000000
VERSION     DAETPZ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.314"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>729
                     /locus_tag="K0W59_004453"
     CDS             <1..>729
                     /locus_tag="K0W59_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HBI3025130.1"
                     /translation="LRRTDDWMDGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWALL
                     RRQAELDGMPAINAKRVYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDG
                     FEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSS
                     PVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMP
                     KPDGLTAAKNLAEAFEHYNEWHPH"
BASE COUNT          180 a          177 c          222 g          150 t
ORIGIN      
        1 ttctcagacg aaccgatgac tggatggatg gccgccgcag tcgtcacact gatgatacgg
       61 atgtgcttct ccgtatacac catgttatcg gagagctgcc cacgtatggt tatcgtcggg
      121 tatgggcgct gcttcgcaga caggcagaac ttgatggtat gcctgcgatc aatgccaaac
      181 gtgtttaccg gatcatgcgc cagaatgcgc tgttgcttga gcgaaaacct gctgtaccgc
      241 catcgaaacg ggcacataca ggcagagtgg ccgtgaaaga aagcaatcag cgatggtgct
      301 ctgacgggtt cgagttctgc tgtgataacg gagagagact gcgtgtcacg ttcgcgctgg
      361 actgctgtga tcgtgaggca ctgcactggg cggtgactac cggcggcttc aacagtgaaa
      421 cagtacagga cgtcatgctg ggagcggtgg aacgccgctt cggcaacgat cttccgtcgt
      481 ctccagtgga gtggctgacg gataatggtt catgctaccg ggctaatgaa acacgccagt
      541 tcgcccggat gttgggactt gaaccgaaga acacggcggt gcggagtccg gagagtaacg
      601 gaatagcaga gagcttcgtg aaaacgataa agcgtgacta catcagtatc atgcccaaac
      661 cagacgggtt aacggcagca aagaaccttg cagaggcgtt cgagcattat aacgaatggc
      721 atccgcata
//
LOCUS       DAETPZ010000308          716 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.315, whole genome shotgun sequence.
ACCESSION   DAETPZ010000308 DAETPZ010000000
VERSION     DAETPZ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.315"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            278..>716
                     /locus_tag="K0W59_004454"
     CDS             278..>716
                     /locus_tag="K0W59_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3025131.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDVATNVALQMYLPDGSTSVTPGTETSNIQLAD"
BASE COUNT          218 a          122 c          148 g          228 t
ORIGIN      
        1 atgccaaata taaattttgt gtatcttttt ctgaaaataa actaaagaaa gggctttata
       61 tatcacgcat atttatttgt tggatagtca ttagatgttg aatcaatgaa ttactcgttc
      121 agttaatatt taaatattca tgaaatctat aaattaaaga tttgtcactt attggattta
      181 gtatgcttga tttaaatggc ggtcaattat tgttattatt aagtctaatg tcatttaaag
      241 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
      301 gtttataact gtggtatgtg caacgtccag cgttatggct gctgatgata atgctatcac
      361 ggatggctca gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      421 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      481 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      541 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      601 tgccaaccag gctaccaccg atgtagcgac gaatgttgct ctacaaatgt atctgccaga
      661 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cggatt
//
LOCUS       DAETPZ010000309          699 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.316, whole genome shotgun sequence.
ACCESSION   DAETPZ010000309 DAETPZ010000000
VERSION     DAETPZ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.316"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..311)
                     /locus_tag="K0W59_004455"
     CDS             complement(<1..311)
                     /locus_tag="K0W59_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBI3025132.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     M"
     gene            complement(375..>699)
                     /locus_tag="K0W59_004456"
     CDS             complement(375..>699)
                     /locus_tag="K0W59_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBI3025133.1"
                     /translation="RNRWFSVMAGPSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTD
                     NKGKTHDVLTGSDDNRHSNTSLAWGAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVG
                     VGYKF"
BASE COUNT          165 a          233 c          155 g          146 t
ORIGIN      
        1 ttcatcacct ccggacgtaa atcaccatcc tttggtgcat tcagaaacgc gttcagtgtc
       61 cccggtgcat cagtcggtgc cacatcaata tccccgacac gggttggctg ccagccgtta
      121 cagtgaagcg caacctcgta ataccccggc tcagcctcaa tcacataagc accgttattg
      181 tccgtcacgc aagtggcaac aacgtgtgcc acaacggtcg gactggttct tctggcccgc
      241 agttcaatcg cacaatttac gacaggttta cccgccccat ctttcagtac acctgaaatc
      301 tttactgcca tattcacccc acaaaaagcc cgcctgaacc agcgggctgt cataacactg
      361 tgttacctgg ctaatcagaa cttataaccg acacccacaa tgaaaccgtc agtgcgccag
      421 tcgccactgc cggaaccttc ataagcaagg tcaatggcca cggattcggt cgggttaaac
      481 tgcacgccag ccccccacgc cagagacgtg ttgctgtggc ggttgtcatc actcccggtc
      541 agcacatcgt gcgttttccc cttgttatca gttacgcgga gataatcccc ggagaaggtc
      601 gacacacggc tgtaagccac gcccgccatc gcatacgcgc tgaaccattc attcacgcgc
      661 acagacggcc ccgccatcac gctgaaccag cggttacgc
//
LOCUS       DAETPZ010000310          670 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.317, whole genome shotgun sequence.
ACCESSION   DAETPZ010000310 DAETPZ010000000
VERSION     DAETPZ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.317"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..280
                     /locus_tag="K0W59_004457"
     CDS             <1..280
                     /locus_tag="K0W59_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HBI3025134.1"
                     /translation="SLNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGFDNGGESA
                     AIIYSLLFTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
     gene            complement(525..>670)
                     /locus_tag="K0W59_004458"
     CDS             complement(525..>670)
                     /locus_tag="K0W59_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3025135.1"
                     /translation="NRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQR
                     LRN"
BASE COUNT          160 a          155 c          174 g          181 t
ORIGIN      
        1 tagcctgaac gtgtactgtc gtaatggcca ggtggaaatc gacaacaaca tcggtgaaaa
       61 cgcgctgcga tcggtggcgg ttggaagaaa aaattatctc ttcttcggtt ttgacaacgg
      121 tggagaaagc gcggcgatta tctacagtct gctgttcacc tgcaaactga acggagtgga
      181 gccggaggac tggttgcgcg aagtgatcgt gaagctcaat gactggccgt cgaaccgagt
      241 gcatgaactg ctgccctgga acttctcagc tgtaaaataa tccttccgct acgtacttct
      301 ctgggcgctt accactcaac ggcatcgtca aaattatcaa agcgcagaac atagatttat
      361 catgtgtttt atggatggct ttctccatca ggcatcgttc gtcacgggaa attggtgcta
      421 tgatcggcat tgatcagtcc ggttggtgat ttgttttgat ttggcgattg atcagatcgc
      481 acaatccggg ctgagttccc tctaagtgat ctaggttgtg tcccttaatt acgtaagcgt
      541 tgataaaaga gtcggatgca gcccagtttc accatcgcca ggtaatttct cgccgttttg
      601 tcgtaacgcg tggcgatgcg gcgatattct ttcagccgcc caaagcaccg ttcaacgaca
      661 ttacgattgc
//
LOCUS       DAETPZ010000311          657 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.318, whole genome shotgun sequence.
ACCESSION   DAETPZ010000311 DAETPZ010000000
VERSION     DAETPZ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.318"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            101..>657
                     /locus_tag="K0W59_004459"
     CDS             101..>657
                     /locus_tag="K0W59_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBI3025136.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDS"
BASE COUNT          236 a          121 c          108 g          192 t
ORIGIN      
        1 caatacctgg agaaagagta caaagcaaac tgtattaaac ttaataagtt atattgtatt
       61 tcactagagt tcttatcgtt gataagaaat tctggttata atgattaaat caacaaatat
      121 acaggtaatc ggttctggta ttatgcatca aataaacaac atacactcgt taactctatt
      181 ttctttgcct gtgagtttga gtccatcttg taatgaatat tatttaaaag tctggagtga
      241 atgggaaaag aacggtaccc caggcgagca acgcaatatc gccttcaata ggctaaaaat
      301 atgtttgcaa aatcaagggg cggaattaaa tctatctgag ttagatttaa aaacattacc
      361 tgatttaccg cctcagataa caacactgga aataagaaaa aacctattaa catatctccc
      421 tgatttccca ccaatgctta aggtaataca tgctcaattt aatcaactgg aaagcttacc
      481 tgccttaccc gagacgttag aagagcttaa tgtgggtgat aacaagataa aagaattacc
      541 atatcttcct gaaactctaa ctcatttacg gatccataat aaccgattgc atattctgcc
      601 actattgcca ccggagctaa aattactgat agtttctgga aacagattag acagcat
//
LOCUS       DAETPZ010000312          636 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.319, whole genome shotgun sequence.
ACCESSION   DAETPZ010000312 DAETPZ010000000
VERSION     DAETPZ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.319"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            88..153
                     /locus_tag="K0W59_004460"
                     /pseudo
     CDS             88..153
                     /locus_tag="K0W59_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            381..629
                     /locus_tag="K0W59_004461"
     CDS             381..629
                     /locus_tag="K0W59_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBI3025137.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          202 a          131 c          147 g          156 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacac
//
LOCUS       DAETPZ010000313          619 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.320, whole genome shotgun sequence.
ACCESSION   DAETPZ010000313 DAETPZ010000000
VERSION     DAETPZ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.320"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(14..>619)
                     /gene="ospZ"
                     /locus_tag="K0W59_004462"
     CDS             complement(14..>619)
                     /gene="ospZ"
                     /locus_tag="K0W59_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HBI3025138.1"
                     /translation="VEHGYFNESPNIYDKKYISGITRSMAQLKIEEFINEKSRRLNYM
                     KTMYSPCPEDFQPISRDEASTPEGSWLTVISGKRPMGQFSVDSLYHPDLHALCELPEI
                     SCKIFSKENSYFLYIIVVFRNDSPQGELRANRFIELYDIKREIMQVLRDESPELKSIK
                     SEIIIAREMGELFSYASEEIDSYIKQMNDRLSQIKARMPVT"
BASE COUNT          189 a          124 c           91 g          215 t
ORIGIN      
        1 caattaatat acattaagta acaggcattc gagccttgat ttgagaaagc ctgtcattca
       61 tttgttttat gtagctatct atttcctctg atgcataact aaaaagttct cccatttcac
      121 gagcaataat aatctcagat ttaatagact ttaactctgg cgactcatct cttaacactt
      181 gcataatctc tctttttata tcatatagtt ctataaaccg atttgcccgt agttctcctt
      241 ggggagaatc atttctgaat acaacaatta tatacaaaaa ataactattt tctttagaga
      301 agatcttaca actaatctct gggagctcac ataatgcatg taagtcagga tggtacaaac
      361 tatccacaga aaattgcccc ataggacgtt ttccagatat aactgtcaac cagcttcctt
      421 ccggtgtact ggcttcgtct ctggaaatag gctgaaaatc ttccggacat ggagaataca
      481 ttgtcttcat ataattaagt cgtctggatt tctcattaat aaactcctct atttttaatt
      541 gtgccatact tctggttata ccagagatat acttcttgtc atatatgttt ggtgattcat
      601 taaagtaacc atgttcaac
//
LOCUS       DAETPZ010000314          614 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.321, whole genome shotgun sequence.
ACCESSION   DAETPZ010000314 DAETPZ010000000
VERSION     DAETPZ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.321"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..183
                     /locus_tag="K0W59_004463"
     CDS             <1..183
                     /locus_tag="K0W59_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3025139.1"
                     /translation="QLEKTGITKDAIRKDMVPALRELMQAKHRAQKAVDELVDCVAEL
                     ETRVGKFEKLVQEALR"
     gene            278..469
                     /locus_tag="K0W59_004464"
     CDS             278..469
                     /locus_tag="K0W59_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HBI3025140.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKE"
BASE COUNT          178 a          132 c          165 g          139 t
ORIGIN      
        1 caactcgaaa aaacgggcat aacaaaagat gctatccgta aagatatggt acccgcgctt
       61 cgggaactga tgcaggcgaa gcatcgcgca cagaaagcag tagatgagct ggtggactgt
      121 gtggcagagc tggaaaccag agtcggaaag tttgaaaaac tggtgcagga ggcgctgcgc
      181 tgatgcgcca tgagtttgtt ttgccctggc ttgtgcgcga tcattatgtc cctggtggaa
      241 agcccgggat aaaaatcacc gaaccggaga gtatttgatg agcgtcaaaa ttcaaacgat
      301 accggaactg cttatccaga cacgaggtaa tatgaccgaa gtttcacgaa tgctgaactg
      361 taatcgcgcc acggtaagaa aatatgctga agataaagag ggcaaagggc acgccattgt
      421 tgatggtgtt ctgattgttc atcgtggatg ggacaggggt aaagagtaag cgccccatca
      481 gcgacgtctt gtgaaaattg tcctgtctgg caacaatcgc gcccatctat attgatggac
      541 acgaacgatg aattcccaga caacaaaaga tattccctgc ttccgttctt atttgcctga
      601 tgccctgcat ttaa
//
LOCUS       DAETPZ010000315          596 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.322, whole genome shotgun sequence.
ACCESSION   DAETPZ010000315 DAETPZ010000000
VERSION     DAETPZ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.322"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..323
                     /locus_tag="K0W59_004465"
     CDS             <1..323
                     /locus_tag="K0W59_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3025141.1"
                     /translation="ARIAREHDINNNLLFKWKSQYEDGLLSDDDIQECMPVPVALTDT
                     PEPTRPVTNPFWRNKHDERPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMK
                     GGIR"
     gene            320..>596
                     /gene="tnpB"
                     /locus_tag="K0W59_004466"
     CDS             320..>596
                     /gene="tnpB"
                     /locus_tag="K0W59_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBI3025142.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQ"
BASE COUNT          145 a          157 c          172 g          122 t
ORIGIN      
        1 ttgcccggat cgccagagaa cacgatatca ataataacct gctgtttaaa tggaaaagcc
       61 agtacgagga cggcttactg agcgatgatg acatacagga atgcatgcct gtcccggtgg
      121 cactgactga tacgccggag ccgaccagac cagttacaaa tcccttctgg cgtaacaagc
      181 atgatgagcg ccctgagggg gctcccggaa acgtcccacg gtgcgagctg catcttaaat
      241 caggtgtggt aaaactgttt gaccctctca ctccggaact gttacgggcg ctaatccgcg
      301 aaatgaaagg gggtatccga tgataacgct gccgaccggt accaaaatct ggatcatcgc
      361 tggcatcaca gatatgcgtt gtggcttcaa tggcctggct tcgaaggtgc agaacacgct
      421 gaaagatgac ccgttctccg ggcatatctt cgtcttccgg ggccgcagtg gcaaaatggt
      481 gaaaatactg tgggccgatc gtgacgggtt atgcctgttc gccaaacgcc tggaacgggg
      541 ccgcttcgtc tggccggtga cccgggaagg gaaagtgcac ctgacgccag ctcagt
//
LOCUS       DAETPZ010000316          566 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.323, whole genome shotgun sequence.
ACCESSION   DAETPZ010000316 DAETPZ010000000
VERSION     DAETPZ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.323"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..297
                     /locus_tag="K0W59_004467"
     CDS             <1..297
                     /locus_tag="K0W59_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HBI3025143.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="K0W59_004468"
     CDS             complement(405..>566)
                     /locus_tag="K0W59_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3025144.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DAETPZ010000317          564 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.324, whole genome shotgun sequence.
ACCESSION   DAETPZ010000317 DAETPZ010000000
VERSION     DAETPZ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.324"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>564
                     /locus_tag="K0W59_004469"
     CDS             <1..>564
                     /locus_tag="K0W59_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HBI3025145.1"
                     /translation="LNWLEKQPEGAVRPVVIESVTKIMACGTTLMGYTQWCCSSPDCC
                     HTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVFTLPCQYWSLVFHNRW
                     LLAEMSRIAADVILEICHQADVEPGIFTVSHTWGRDQQWHPHIHLSTTAGGVTSGHTW
                     KNLHFYARKVMSMWRYRITRLLSRKYPD"
BASE COUNT          128 a          147 c          166 g          123 t
ORIGIN      
        1 cttaactggc tggagaaaca accggaaggg gcagtccgtc cggtggtaat tgaatcagtg
       61 acaaaaatca tggcctgcgg gaccacactg atgggctaca cgcagtggtg ttgttcatca
      121 ccggactgtt gccatacaaa aaaggtctgc ttccggtgta aaagccgctc ctgcccgcac
      181 tgcggggtga aggctggcgc acagtggata cagtatctgc tgagcctggt ccccgactgc
      241 ccgtggcagc atattgtgtt cacacttccc tgccagtact ggtccctggt gttccacaac
      301 cggtggttac tggcagagat gagccgcatt gcagcggatg tgatactgga aatctgccat
      361 caggcagatg tggagccggg gatattcacg gtgagccaca catgggggcg tgaccagcag
      421 tggcatccgc atatccattt atcgacaact gccggtggtg tgacgtcggg ccacacctgg
      481 aaaaatcttc atttttacgc ccgtaaggtg atgagcatgt ggcgctaccg gataacacgg
      541 ttgctgtcac ggaaataccc tgac
//
LOCUS       DAETPZ010000318          563 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.325, whole genome shotgun sequence.
ACCESSION   DAETPZ010000318 DAETPZ010000000
VERSION     DAETPZ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.325"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            285..563
                     /locus_tag="K0W59_004470"
                     /pseudo
     CDS             285..563
                     /locus_tag="K0W59_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          149 a          156 c          125 g          133 t
ORIGIN      
        1 ttccctcgcg atattaacgt aatatcttaa ttgtatgcac cgccagaatg cgttcagtgc
       61 gatcacggca gcagacaggt aaaaatagca acaaaccacc cgaaaaaccg ccgcgatcgc
      121 gtatgataaa ttttaaccac atgcatacct gtgcagcaag gcgaatcacg cacggatttc
      181 cagccagaga cacctgaaac gtaaccggct catttaaacc gtctggtctg ttacctccgg
      241 ttccacaaaa ataatgtcca tcattttgaa gtggacacta tcgtatggaa taccggacct
      301 gaatcactga agctttacgc cttcatttcg aagaacattt accacgggtt gtggccggac
      361 gtcgcctggg cgtaccaaaa tcaactgtct gtggtatgtt cgtgcgcttc cgcaacgctg
      421 gcctttcatg gcctctgccc gcaggtatgt cggagcagga acttgatgcc cttctttacg
      481 gaagtgcctc cacagtacct gtcgtactta ctgaaagcac cgttatgccc aaactcccgg
      541 tggtaaaaaa acgtccccgg cgg
//
LOCUS       DAETPZ010000319          553 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.326, whole genome shotgun sequence.
ACCESSION   DAETPZ010000319 DAETPZ010000000
VERSION     DAETPZ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.326"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(28..>553)
                     /locus_tag="K0W59_004471"
     CDS             complement(28..>553)
                     /locus_tag="K0W59_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBI3025146.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGAAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT          144 a          139 c          134 g          136 t
ORIGIN      
        1 accagtattt cttcggagtg aagaatatta ccaagtatat ttaacaccca cgttcgcgga
       61 ccagtcttga tctacgtcac caccaccgag gtagttagca tcggtatagg cgctgaagtt
      121 cttggtgaag ctgaactgag tacccagacc aacacgtacc gcagcccctt cagtaccgtt
      181 atcgatggaa tcaccgttca catcgttatc gttgttagag tcgtcgtaga cgtaagccag
      241 tttgaagtac ggagtcagag cctgatcttc gctgtaggtg aaggtataac ctgcatctac
      301 acccagttca taacgcatgc tgtcgtaaga ctgaccgtca actttcatgt cgttgctcag
      361 ctggtagtca tcaccagact ggaacagacc agaaatgctg ccgtaaggag tcacgtaacc
      421 agcatcaccc agtttgaagt cgtaaccggc tttcaaaccg aagccccaag cgtcggagtt
      481 ggtgctaccg tcaacgtaag taccgttgct catggttgca gacaggtcgt tgttgaagtg
      541 agagtagctc aag
//
LOCUS       DAETPZ010000320          543 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.327, whole genome shotgun sequence.
ACCESSION   DAETPZ010000320 DAETPZ010000000
VERSION     DAETPZ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.327"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(88..237)
                     /gene="hokB"
                     /locus_tag="K0W59_004472"
     CDS             complement(88..237)
                     /gene="hokB"
                     /locus_tag="K0W59_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HBI3025147.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(337..477)
                     /locus_tag="K0W59_004473"
                     /pseudo
     CDS             complement(337..477)
                     /locus_tag="K0W59_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          141 a          127 c          126 g          149 t
ORIGIN      
        1 aacatgcacg aactgaagtg taaaaggggt gccatgagaa aatgaatctg ctagtcagat
       61 gcgcggggaa aatccccgcg cttgccctta cctggacgtg caggccatga gcgcagcaac
      121 ctccttatca ccgtcccgga accgcagttc gtagagcgtt tgtcgggtca ggagtgtgaa
      181 tgccagaatc gtaatgcaga taatgagcag acacaccacc agagggttgc gcttcatagc
      241 ctttctcctt gccggatggc gggtaagagg ctaacatctg aattgcttgg ttcattcgtt
      301 ggcctcggtt tgtactgacc ccaaaaagtt ggacagttaa acacgaggca tataggtctg
      361 attccgatat tcatttggag tcagaccttt taatttcagg ctaattcttc tgctgttgta
      421 gtattcaata tattccgtaa cagcatcctt cagttcgctt atattactga actcattaga
      481 ctggccccct gaatctccag acaaccagta tcacttattt aagtgatagt cttaatacta
      541 gtt
//
LOCUS       DAETPZ010000321          527 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.328, whole genome shotgun sequence.
ACCESSION   DAETPZ010000321 DAETPZ010000000
VERSION     DAETPZ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.328"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..>527)
                     /locus_tag="K0W59_004474"
     CDS             complement(<1..>527)
                     /locus_tag="K0W59_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBI3025148.1"
                     /translation="DCADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNNAADTHV
                     VDVYNIGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNSSEVFNNT
                     ITVKDSTVTSGSWTDEGTTGWFGNTGNASDYSGKSNFVTVDTDGDGVADSTIASWDDV
                     ALAVVAHPNADNAMQ"
BASE COUNT          150 a          125 c          105 g          147 t
ORIGIN      
        1 tctgcatagc attatcagca ttcggatgtg caactacagc taatgcaaca tcatcccagc
       61 ttgcaatagt gctatcagca acaccatctc cgtcagtatc tacggtgaca aagttagatt
      121 taccactata atcgctggca ttacctgtat taccgaacca accagtagta ccttcatcag
      181 tccatgaacc agaggttacg gtagaatctt taacagtgat ggtgttatta aacacttcgc
      241 tgctgtttac gccagtgctg acagtgttgt cgtcaatatc ttcccactca taaccctgag
      301 tcaacgtaat acccgcaacg tgagagttat tagtgatgga cagatcaact tcctgatcca
      361 gagtaatagc cgtaccaatg ttgtaaacgt ctacaacatg agtatcagcg gcattattgt
      421 aagtgccgtt atatgtgtag tgttcgtagt tgtcgtcgat cgttgaatta tcaacagtca
      481 gcgccagacg gtcatggtag taatcatccg cacgatcagc acaatca
//
LOCUS       DAETPZ010000322          525 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.329, whole genome shotgun sequence.
ACCESSION   DAETPZ010000322 DAETPZ010000000
VERSION     DAETPZ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.329"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            42..>525
                     /locus_tag="K0W59_004475"
     CDS             42..>525
                     /locus_tag="K0W59_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3025149.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYRRMLYIEFTDNMR
                     Y"
BASE COUNT          129 a          132 c          135 g          129 t
ORIGIN      
        1 agatccaggg ggtaatctgc tctcctgatt caggagagct tatggtcact tttgagacag
       61 ttatggaaat taaaatcctg cacaagcagg gaatgagtag ccgggcgatt gccagagaac
      121 tggggctctc ccgcaatacc gttaaacgtt atttgcaggc aaaatctgag ccgccaaaat
      181 atacgccgcg acctgctgtt gcttcactcc tggatgaata ccgggattat attcgtcaac
      241 gcatcgccga tgctcatcct tacaaaatcc cggcaacggt aatcgctcgc gagatcagag
      301 accagggata tcgtggcgga atgaccattc tcagggcatt cattcgttct ctctcggttc
      361 ctcaggagca ggagcctgcc gttcggttcg aaactgaacc cggacgacag atgcaggttg
      421 actggggcac tatgcgtaat ggtcgctcac cgcttcacgt gttcgttgct gttcccggat
      481 acagacgaat gttgtacatc gaattcactg acaatatgcg ttatg
//
LOCUS       DAETPZ010000323          523 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.330, whole genome shotgun sequence.
ACCESSION   DAETPZ010000323 DAETPZ010000000
VERSION     DAETPZ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.330"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
BASE COUNT          120 a          128 c          143 g          132 t
ORIGIN      
        1 atacccgatt agttgttaat catatttaat catagtgatt actttcagac ggaacaaggc
       61 aaataatgcg agtcagcgca tataaggcgg catacggtgg ttacatgtag cacaggtgta
      121 aaaccgccga agcccggcgc aagccggtac ggtcgtccga atatcaccag tccccctccg
      181 gctctgcgag gccatgcggc ctgtcagcga tgaaaatgcc tctgtgcgcg gtcggagtgg
      241 tcccgacaag ggtttacccg cagtcggggc gtatctccgc gttagcgggc cgtgagggcc
      301 gcttacgagc gtgtatgaga gacgtacagt agttttgtcc gctcacacca gtacaaacca
      361 gtcgaaatac cggtaacgac gattactctt gtctctgatg ccggtggact acgagatttt
      421 atttactggc gtcctgatgc ggtcaggact ggtatggaac ctgtttatgt gatacttaat
      481 gctcttctgg attccgggcg atttacgcgt aaacttattc tgt
//
LOCUS       DAETPZ010000324          481 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.331, whole genome shotgun sequence.
ACCESSION   DAETPZ010000324 DAETPZ010000000
VERSION     DAETPZ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.331"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            72..335
                     /locus_tag="K0W59_004476"
                     /pseudo
     CDS             72..335
                     /locus_tag="K0W59_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            381..>481
                     /locus_tag="K0W59_004477"
     CDS             381..>481
                     /locus_tag="K0W59_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="with Hns involved in transcriptional regulation of
                     hemolysin; non-specific DNA-binding protein which affects
                     the production of multiple proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI3025150.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLS"
BASE COUNT          136 a          104 c          110 g          131 t
ORIGIN      
        1 cttcggcttt gaactcttta gagtaacgtt gggtttttct gctcattatt agctccttct
       61 gatgccattc tatttcagga aggagtgtcc gttaaactca ggctacctca tattttcagc
      121 gagatcgtta tggtcgtatt ctcgggcagg tttatgcacc agacggaatg aatattaatc
      181 agtttatggt ccgggccggg gctgcctggg tatatgagca gtacaacaca gatcccgtgc
      241 tacctgtcct tcagaacgaa gccagacaac aaaaacgtgg gctctggtca gatgctgatc
      301 cggttccccc ctggatatgg aggcaccgta aataaagcga tggcaatcct atcattcatc
      361 tgttctcagc gggaataaaa atggcaaaaa caaaacagga atggctgtat cagttacggc
      421 gatgttcatc agtcaatacg ctggaaaaaa ttattcataa aaacagagac agtttatcaa
      481 a
//
LOCUS       DAETPZ010000325          474 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.332, whole genome shotgun sequence.
ACCESSION   DAETPZ010000325 DAETPZ010000000
VERSION     DAETPZ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.332"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..411)
                     /locus_tag="K0W59_004478"
     CDS             complement(<1..411)
                     /locus_tag="K0W59_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HBI3025151.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEADVQTKQERNLTGVQTDVGVVFEHD"
BASE COUNT          111 a          117 c           94 g          152 t
ORIGIN      
        1 atcatgttcg aacaccactc caacatctgt ttgaacacct gttaaatttc tctcttgttt
       61 tgtttgaaca tctgcttcct ttctgcttct tctggcctga acagatgctt ttcctgcggc
      121 tgattttttg gttaattttt ctctgacaga tgccagatct tcctcaatcc gaagatgcat
      181 ccattcgtcg ccgttatcac aaaaaaactc ccgcaaggat ggttcaacat caacccatcg
      241 ctcgttagtc agacgggcaa tttttgccaa cctgtttttg ggtattggct ttcctgtttg
      301 ccagtaattg aacatcagca acaaatacgc gccgtgctcc tctgctgaca aatgcatggt
      361 gtcagccaga taatcagcta tgtacagttg catgtatggt aatgcggcca taattgcccc
      421 gtatgatgct acccggttgc ttagaataag tgaggtagcc tgagtttaac ggac
//
LOCUS       DAETPZ010000326          464 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.333, whole genome shotgun sequence.
ACCESSION   DAETPZ010000326 DAETPZ010000000
VERSION     DAETPZ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.333"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>464
                     /locus_tag="K0W59_004479"
     CDS             <1..>464
                     /locus_tag="K0W59_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3025152.1"
                     /translation="QHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEHWGYVGAKSRQ
                     RWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKL
                     HVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHY"
BASE COUNT          113 a          111 c          133 g          107 t
ORIGIN      
        1 ctcaacacgg ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac
       61 aaccgggcag tgatgtgatt gtctgcgctg aaatggacga acattggggc tacgtcggtg
      121 ctaaatcacg tcagcgctgg ctgttttacg cgtatgacag gatacggagg acggttgtgg
      181 cgcacgtctt cggtgaacgc actctggcca cactggagcg tcttctgagc ctgctgtcgg
      241 cctttgaggt cgtggtatgg atgacggatg gctggccgct gtatgaatca cgcctgaagg
      301 gaaagctgca cgttatcagc aagcgttaca ctcagcgcat tgagcgacat aatctgaatc
      361 tgagacaaca tctggcaagg ctgggacgga agtcactgtc gttctcaaaa tcggtggagc
      421 tgcatgacaa ggtcatcggg cattatctga acataaaaca ctat
//
LOCUS       DAETPZ010000327          463 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.334, whole genome shotgun sequence.
ACCESSION   DAETPZ010000327 DAETPZ010000000
VERSION     DAETPZ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.334"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>463
                     /locus_tag="K0W59_004480"
     CDS             <1..>463
                     /locus_tag="K0W59_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HBI3025153.1"
                     /translation="KRGGHEHVAIPNYLERQFAVTEPNQVWCGDVTYIWTGKRWAYLA
                     VVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQ
                     LLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAIT"
BASE COUNT          111 a          111 c          146 g           95 t
ORIGIN      
        1 ataaacgtgg tggtcatgaa catgttgcta tccctaacta ccttgaaagg cagttcgccg
       61 tgaccgagcc aaatcaggtg tggtgcggtg atgtgaccta tatctggacg ggtaagcgct
      121 gggcgtacct cgccgttgtt ctcgacctgt tcgcaagaaa accagtgggc tgggccatgt
      181 cgttctcgcc ggacagcagg ctcaccatga aagcgctgga aatggcatgg gaaacccgtg
      241 gtaagcccgg cggggtgatg ttccacagcg atcagggcag tcattatacg agcaggcagt
      301 tccggcagtt attgtggcga taccagatca gacagagtat gagccggcgc ggaaactgct
      361 gggataacag cccaatggaa cgcttcttca ggagtctgaa gaacgaatgg atgccggtgg
      421 tgggttacgt aagcttcagc gaggcagctc acgccataac gga
//
LOCUS       DAETPZ010000328          443 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.335, whole genome shotgun sequence.
ACCESSION   DAETPZ010000328 DAETPZ010000000
VERSION     DAETPZ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.335"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            213..>443
                     /locus_tag="K0W59_004481"
     CDS             213..>443
                     /locus_tag="K0W59_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HBI3025154.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          140 a          105 c           62 g          136 t
ORIGIN      
        1 tatcgaacct cgcttaccaa cgcgttgtca gtcatctccc ccctccttgg tgcatcgtta
       61 tatccgtgat gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt
      121 cagaaaacaa acttattttt attaaaatca attctttttc tttgaacatt caactgcgat
      181 atttgtaaaa acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat
      241 tccccgcata tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc
      301 aatacgcacg cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatggcagac
      361 gtactgttga gatccacaaa ctagatctct ctgactggca agcattcaat aagttagcaa
      421 cgaagtgtaa tgcttacgat gga
//
LOCUS       DAETPZ010000329          439 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.336, whole genome shotgun sequence.
ACCESSION   DAETPZ010000329 DAETPZ010000000
VERSION     DAETPZ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.336"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            71..439
                     /locus_tag="K0W59_004482"
                     /pseudo
     CDS             71..439
                     /locus_tag="K0W59_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          124 a          100 c          119 g           96 t
ORIGIN      
        1 cagacaacca gtatcactta tttaagtgat agtcttaata ctagttttta gactagtcat
       61 tggagaacag atgattgatg tcttagggcc ggagaaacgc agacggcgta ccacacagga
      121 aaagatcgca attgttcagc agagctttga accggggatg acggtctccc tcgttgcccg
      181 gcaacatggt gtagcagcca gccagttatt tctctggcgt aagcaatacc aggaaggaag
      241 tcttactgct gtggccgccg gagaacaggt tgttcctgcc tctgaacttg ctgccgccat
      301 gaagcagatt aaagaactcc agcgcctgct cggcaagaaa acgatggaaa atgaactcct
      361 caaagaagcc gttgaatatg gacgggcaaa aaagtggata gcgcacgcgc ccttattgcc
      421 cggggatggg gagtaagct
//
LOCUS       DAETPZ010000330          405 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.337, whole genome shotgun sequence.
ACCESSION   DAETPZ010000330 DAETPZ010000000
VERSION     DAETPZ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.337"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
BASE COUNT          122 a          104 c           75 g          104 t
ORIGIN      
        1 ccccccgaca gacctgtgct atcggaaaga aaaaaggcca tcagcaaaac tctgatgacc
       61 tgaatcacct ttaccaatat tgtatgaaaa aacacgcacg actaattgac aataattttc
      121 atttccatta aaaaagatcg cgtagcattc ttaattcatg aggaacttta cccgccagca
      181 atctgagtag cgaaagctgt tcatccccaa tgtttttgct ggcgggtcct tttttctacg
      241 gtcctcctcc ccggaggggg gcatttcatt atttttctaa tcgttactgg tgagaaggcg
      301 gaagaaaaag ccagttcttc gcccctaaat aacagcatga ttccagtcca tgcaaaatct
      361 gatttccagc tcacacatac cgagcgctgt acaaaaaacc gccag
//
LOCUS       DAETPZ010000331          400 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.338, whole genome shotgun sequence.
ACCESSION   DAETPZ010000331 DAETPZ010000000
VERSION     DAETPZ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.338"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            150..335
                     /locus_tag="K0W59_004483"
                     /pseudo
     CDS             150..335
                     /locus_tag="K0W59_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          115 a           95 c          105 g           85 t
ORIGIN      
        1 acggtggtcg gtttacgctt accgcaggac aggtggtaac tgaaaaattg ctgaggcgac
       61 tggaaacaga agggcaagat cgattccgac agcagtgatg gtcagctacc tcatcgataa
      121 ctttgcaaca gtgcccttct tgaagcatca tgcttcatga agaaaaactg gcacgtcatc
      181 aacgtaaaca ggcgatgtat acccgaatgg tagccttccc ggcggtgaaa atgttcgaag
      241 agtatgactt cacgttcgcc accggagcac cgcagaagca gctccagtcg ttacgctcac
      301 tcagcttcat agagcgcaat gaaaacatag ttttacaggg gacatctgac atcacaaatc
      361 cccgggtggg gatttgtgta taagagacag cccatggagg
//
LOCUS       DAETPZ010000332          399 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.339, whole genome shotgun sequence.
ACCESSION   DAETPZ010000332 DAETPZ010000000
VERSION     DAETPZ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.339"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>399
                     /locus_tag="K0W59_004484"
     CDS             <1..>399
                     /locus_tag="K0W59_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3025155.1"
                     /translation="LQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRLAADIAQALAEQ
                     TVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPVLTSMPGVGVRT
                     AARLLTEVACRAFASAAHLAAYAGLAPVTR"
BASE COUNT           77 a          136 c          106 g           80 t
ORIGIN      
        1 ttctccagcg atatccctca ccagaaaaac tcgcttcgct gggtgagaag aagctggcag
       61 cccagctctg caaacttgcg cctcgtctgg gtaaacgcct tgcagcagac atagctcagg
      121 cactggccga acaaaccgtc gtcgttcccg gcacgaatgc cgctgccgta gtactgccac
      181 gtctggcact ccagctcatc acgctgcgta agcaaagaga cgaggtggcg cttgaggtag
      241 aacagcgagt tcttgctcac cctctttacc cggtcctgac cagtatgccc ggagtcggtg
      301 tcaggaccgc agccagactc ctcaccgagg tcgcctgccg cgccttcgcc tctgccgcac
      361 atctcgctgc ttatgctggc cttgcgccgg taactcggc
//
LOCUS       DAETPZ010000333          393 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.340, whole genome shotgun sequence.
ACCESSION   DAETPZ010000333 DAETPZ010000000
VERSION     DAETPZ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.340"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>393
                     /locus_tag="K0W59_004485"
     CDS             <1..>393
                     /locus_tag="K0W59_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI3025156.1"
                     /translation="ALIEQWRQQFPIEAMCQVFGVSRSGYYNWVQHEPSDRKQSDERL
                     KLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTN
                     PNHNLPVAPNLLNQTFAPTAPNQVWVADL"
BASE COUNT          112 a           99 c          106 g           76 t
ORIGIN      
        1 cgcgttaatc gaacaatggc gacaacaatt tcccattgaa gcgatgtgtc aggtatttgg
       61 tgtatccagg agcggttatt acaactgggt acagcatgaa ccctcagaca gaaaacaaag
      121 tgatgagcgg ctaaaactgg agattaaggt ggcacatatc cgcactcgcg aaacatatgg
      181 aacccggcgg ctccagacgg agctggcaga gaatggcatc atcgttggtc gtgaccgact
      241 ggcacgtctt cgtaaggagc tgaggctacg ctgtaagcag aaacgcaagt tcagagcgac
      301 tacgaacccg aaccacaatc tgccagttgc gccaaatctg ctgaaccaga cgttcgctcc
      361 tacagcacca aatcaggtct gggtggcgga cct
//
LOCUS       DAETPZ010000334          375 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.341, whole genome shotgun sequence.
ACCESSION   DAETPZ010000334 DAETPZ010000000
VERSION     DAETPZ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.341"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            17..375
                     /locus_tag="K0W59_004486"
                     /pseudo
     CDS             17..375
                     /locus_tag="K0W59_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT           83 a          108 c           99 g           85 t
ORIGIN      
        1 tttttgaggt gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga
       61 cggggtggtg cgtaacggca aaagcaccgc cggacatcag cgctatctct gctctcactg
      121 ccgtaaaaca tggcaactgc agttcactta caccgcttct caacccggta cgcaccagaa
      181 aatcattgat atggccatga atggcgttgg atgccgggca accgcccgca ttatgggcgt
      241 tggcctcaac acgattttcc gccatttaaa aaactcaggc cgcagtcggt aacctcgcgc
      301 atacagccgg gcagtgacgt catcgtctgc gcggaaatgg acgaacagtg gggatacgtc
      361 ggggctaaat cgcgc
//
LOCUS       DAETPZ010000335          364 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.342, whole genome shotgun sequence.
ACCESSION   DAETPZ010000335 DAETPZ010000000
VERSION     DAETPZ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.342"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..>364
                     /locus_tag="K0W59_004487"
     rRNA            1..>364
                     /locus_tag="K0W59_004487"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          107 a           80 c          108 g           69 t
ORIGIN      
        1 gttaagcgac taagcgtaca cggtggatgc cctggcagtc agaggcgatg aaggacgtgc
       61 taatctgcga taagcgtcgg taaggtgata tgaaccgtta taaccggcga tttccgaatg
      121 gggaaaccca gtgtgattcg tcacactatc attaactgaa tccataggtt aatgaggcga
      181 accgggggaa ctgaaacatc taagtacccc gaggaaaaga aatcaaccga gattccccca
      241 gtagcggcga gcgaacgggg agcagcccag agcctgaatc agtgtgtgtg ttagtggaag
      301 cgtctggaaa ggcgtgcgat acagggtgac agccccgtac acaaaaatgc acatactgtg
      361 agct
//
LOCUS       DAETPZ010000336          359 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.denovo.343, whole genome shotgun sequence.
ACCESSION   DAETPZ010000336 DAETPZ010000000
VERSION     DAETPZ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.denovo.343"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>359
                     /locus_tag="K0W59_004488"
     rRNA            <1..>359
                     /locus_tag="K0W59_004488"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           97 a           76 c          106 g           80 t
ORIGIN      
        1 accttttgta taatgggtca gcgacttata ttctgtagca aggttaaccg aataggggag
       61 ccgaagggaa accgagtctt aactgggcgt taagttgcag ggtatagacc cgaaacccgg
      121 tgatctagcc atgggcaggt tgaaggttgg gtaacactaa ctggaggacc gaaccgacta
      181 atgttgaaaa attagcggat gacttgtggc tgggggtgaa aggccaatca aaccgggaga
      241 tagctggttc tccccgaaag ctatttaggt agcgcctcgt gaattcatct ccgggggtag
      301 agcactgttt cggcaagggg gtcatcccga cttaccaacc cgatgcaaac tgcgaatac
//
LOCUS       DAETPZ010000337        10830 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.guided.116, whole genome shotgun sequence.
ACCESSION   DAETPZ010000337 DAETPZ010000000
VERSION     DAETPZ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10830
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.guided.116"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            59..1103
                     /locus_tag="K0W59_004489"
                     /pseudo
     CDS             59..1103
                     /locus_tag="K0W59_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1165..1779
                     /locus_tag="K0W59_004490"
                     /pseudo
     CDS             1165..1779
                     /locus_tag="K0W59_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1836..3293)
                     /gene="pepD"
                     /locus_tag="K0W59_004491"
     CDS             complement(1836..3293)
                     /gene="pepD"
                     /locus_tag="K0W59_004491"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBI3025157.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3555..4013
                     /gene="gpt"
                     /locus_tag="K0W59_004492"
     CDS             3555..4013
                     /gene="gpt"
                     /locus_tag="K0W59_004492"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBI3025158.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4105..5349
                     /gene="frsA"
                     /locus_tag="K0W59_004493"
     CDS             4105..5349
                     /gene="frsA"
                     /locus_tag="K0W59_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBI3025159.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5407..5808
                     /gene="crl"
                     /locus_tag="K0W59_004494"
     CDS             5407..5808
                     /gene="crl"
                     /locus_tag="K0W59_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBI3025160.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5847..6902)
                     /gene="phoE"
                     /locus_tag="K0W59_004495"
     CDS             complement(5847..6902)
                     /gene="phoE"
                     /locus_tag="K0W59_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBI3025161.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            7190..8293
                     /gene="proB"
                     /locus_tag="K0W59_004496"
     CDS             7190..8293
                     /gene="proB"
                     /locus_tag="K0W59_004496"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBI3025162.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDHNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            8305..9558
                     /gene="proA"
                     /locus_tag="K0W59_004497"
     CDS             8305..9558
                     /gene="proA"
                     /locus_tag="K0W59_004497"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBI3025163.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9673..9748
                     /locus_tag="K0W59_004498"
     tRNA            9673..9748
                     /locus_tag="K0W59_004498"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9706..9708,aa:Thr,seq:cgt)
     gene            complement(9763..>10830)
                     /locus_tag="K0W59_004499"
     CDS             complement(9763..>10830)
                     /locus_tag="K0W59_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBI3025164.1"
                     /translation="TLTRWPVFVDHFKAWRVKNLGESPKKLFKEACIRWLREKSDKKS
                     IDDDKSIISFWMLHFREAILSDITTEKIMEAVDGMENRRHRLNWEMSRDRCLRLGNPV
                     PEYKPKLASKGTKTRHLAILRAILNMAVEWGWLDRAPKISTPRVKNGRIRWLTEEESK
                     RLFAEIAPHFFPVVMFAITTGLRRSNVTDLEWSQVDLDKKMAWMHPDETKAGNAIGVP
                     LNETACQILRKQQGLHKRWVFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGI
                     SNFRFHDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDA
                     IISRNGTNTAQEENVVYLNAR"
BASE COUNT         2582 a         2705 c         2828 g         2715 t
ORIGIN      
        1 aattcaagtc gcagcacatg caacttgaag tatgacgagt ctatttacgg aatggtatat
       61 gggcaattat attcgtccct tatccgatgc ggtatttacc atcgcatctg atgacctgtg
      121 gatcgagagt ttagcgatcc aacaattaca caccacggca aatttaccca acatgcagcg
      181 cgtagtgggg atgccagatt tacaccccgg acgcggctac ccgattggcg cagcgttctt
      241 ctccgcaggc cgtttttacc cagcactggt cggcaatgat atcggctgtg gcatggcgct
      301 atggcaaaca gatattctcg ctcgcaaata caacgccgat aagtttgaaa agcgattatc
      361 agctttggat gacgttgctg aagaaagctg gctggaggaa aacctgccgt cagcatttgc
      421 acagcatccg tggcgcagct cgcttggctc tatcggtggt ggcaatcatt tcgctgaact
      481 gcaacaagtt gatcaaatca tcaacgctga actgtttgca ctggcaggtc tggatgcgca
      541 gcatctgcaa ctgctggtcc atagcggctc gagggagcta gggcagtcta ttttacagcg
      601 gcatattgcc tcgttttcgc atcatggttt gcctgaaggc agtgacgacg cgctggcgtt
      661 tgcgcgtttt aatcgccacc tgatcgcttt gcgcataatg caacaggtta aggccaccgg
      721 aagtccggtt ctggatgtgg cgcataactt tgttagcgcg tgtcgaatcg gtgatcaaca
      781 aggggtctta catcgtaaag gtgccacgcc tgatgactgt ggtctggtgg ttattcccgg
      841 ttcacgcggt gattactcca ggctggttca ggcccgtcag gagtgaggaa acattgcatt
      901 cgctggcgca tggggctggg cgtaaatggg ggcgcaccga gtgtaaaggg cgtctggcag
      961 cgaaatacac agcgacgcaa ctctcgcgta ctgaacttgg cagtcgggta atttgtcgcg
     1021 ataaacaact catcttcgaa gaagcgccac aagcctataa atcggctgaa agtgtggtgc
     1081 aatgtctggg caggccggat taattattcc tgtcgcgcgg ctgcgtccgg tgctaacgct
     1141 caaaaacagt ggagggaaaa aaggatgatc ttgctataac tctcctcagc gcaggggccg
     1201 gaagaatgtt gtctcgcagt gaaaaaggcg ctggacaggc taattaaaga agctgcccga
     1261 caagacgtcg cggtaacggt gctggaaaca gaaacaggcc gctactctga cacgctgcgt
     1321 tcggcgctga tttctctgga tggcgacaac gcatgggcgt taagcgaaag ctggtgtggc
     1381 actattcagt agatttgccc gagcccgtat cgcccacatc acgggcgtaa aaactggttt
     1441 ctgggcattg ggcgttttac cgctgatgag caggagcaat ccgatgcgat ccggtatgag
     1501 acgctgcgtt cgtcggggcc gggcggtcaa catgtcaata aaaccgactc ggcggtacgc
     1561 gccacgcatt tggcatccgg tattagcgtg aaggttcagt cagagcgcag ccagcatgct
     1621 aacaaacggc tggcgcgatt gctgattgcc tggaagctgg atcagcagca ataggaaaat
     1681 agcgcgtagc tgaaatcaca gcggcgaatg ttccatcacc agattgaacg tggcaacccg
     1741 cgacgaacgt ttacagggat ggcttttatc gaaggataat gaaggatgaa actgccggaa
     1801 ggcgattaaa cgccatccgg cagcaaatca aataattact tcgccggaat ttctttcagc
     1861 agttcagtca gcagtgtcca gtaatgacct acgctttcga tgtgaacttg ctcatccgga
     1921 gagtgtggac cggtgatagt tggcccgata gaaaccatgt ccatttccgg atacggtttt
     1981 ttgaacagac cacattccag gcccgcgtgg ataatctgga tgttcggcgt cttgttgaac
     2041 aggcgctgat aggtttcacg taccagatgc atcaccggag aattagcgtc cggctgccag
     2101 ccaggatatg cgcctttcgc ttcggttttc gcgccagcca gtttacccag cgaatccagc
     2161 atgctcacca cgtagtcttt accgctgtcg atcagtgaac ggatcaggca gtgaatttct
     2221 acgttattgt cagtcatggt caccacaccg acgttcaggg aggtttcgac cacacctttt
     2281 gccacgtcgg aattgcggat cacaccgttc ggggtggcgt tcagcagacg aataaaggta
     2341 tcgcgagatt tcgcaatcag ggcagcttta tcgttcgcta cagagtccag caacaaggcc
     2401 agatttttct ctttctctgc cagctcgttt ttcaggatct cctgataggt attcaccaga
     2461 gatttcagtg cgtcgacttt atcagctgtg acagcaatgg tcgcaaaggc ttcacgcggg
     2521 atggcgttac gcagtgtgcc gccgttgaaa tcgataaggc gcaggtccag ttcttctgca
     2581 tgacccgcca ggaagcgcac cagcagtttg ttggcattac ccaggccaac gtggatttca
     2641 ccgccggagt gaccgccttt cagacctttt aaggttaact tgaaggtttc aaaaccagct
     2701 ggaaccgctt cacgatctaa atgcaggttg gaggtgaagt cgataccccc cgcacaaccc
     2761 atgtagattt caccttcttc ttcggagtcg gtgttaatca gaatatcagc ctgcaaccag
     2821 ttgctctgta agccgaacgc accgtccata ccggcttctt cggtcatggt cagcagcact
     2881 tccagcgggc cgtgaaccac gttttcgtca gccagaaccg ccagtgcaga ggccatacca
     2941 atgccgttat ccgcacccag cgtggtgccg cgcgctttaa cccattcgcc atcaatataa
     3001 ggctggatag gatctttcgt gaagtcatgc acggtgtcgt tatttttctg cggcaccata
     3061 tcgaggtggg cctgtaagac gaccggttta cgattttcca tacctgcggt agcaggttta
     3121 cgaatcagga tattacctac ctgatcgcgt tcgacatgga aacctttctc ttttgcccaa
     3181 ccaacaatgt attcagcgag ttgctcttca tgataggacg ggtgaggaat agaacagatt
     3241 ttggcaaaaa tatcccacag cggctgtgga gataattgag acagttcaga cacgttaagt
     3301 ctccttgtcg atcacccgca aaacagtatt gcaggtcaca gggttagcag aaaatgttgt
     3361 caacacaaga caggcttgcg agatatgtat tgagaatacc actttatccc gcgtcaggga
     3421 gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagata
     3481 tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg
     3541 ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg
     3601 cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa
     3661 gccgtggcgg tctggtaccg ggggcgttac tggcgcgtga actgggtatt cgtcatgtcg
     3721 ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac
     3781 gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta
     3841 ctgctgttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac
     3901 cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggatcg
     3961 aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc
     4021 aacgcctggc attgccgggc gttgttcttt ttaacttcag gcaggttaca atagtttcca
     4081 gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa
     4141 ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca cggtgcacaa
     4201 ccgcctgtgc agtcggctct tgatggtaaa accatccctc actggtatcg catgattaac
     4261 cgtctgatgt ggatctggcg cggtattgac ccacgtgaaa tcctcgacgt ccaggcacgt
     4321 attgtgatga gcgatgctga acgtaccgac gatgatttat acgataccgt gattggctac
     4381 cgtggtggca actggattta tgagtgggcc acccaggcga tggtgtggca acaaaaagcc
     4441 tgtacggaag aagatccgca actcagtggt cgacactggc tgcatgcggc tacgttgtac
     4501 aacattgccg cctatcctca tctgaaagga gatgacctgg ccgagcaagc gcaggcttta
     4561 tcaaaccgcg cctatgaaga ggccgcccag cgtctgccgg gcacgatgcg gcagatggag
     4621 tttaccgtac ccggcggtgc gcccatcacc ggctttttgc atatgccgaa aggcgatggc
     4681 ccgttcccga cagtattaat gtgtggtggt ctggatgcga tgcagacgga ctattacagc
     4741 ctgtatgaac gttattttgc gccgcgcggc attgcgatgc tgactattga tatgccgtcg
     4801 gtgggctttt cttcaaaatg gaagctcacc caggactcca gcctgttgca tcagcacgtc
     4861 ttaaaggcgc tgcctaacgt accgtgggtg gatcacactc gcgtcgcggc ctttggtttc
     4921 cgtttcggcg ctaacgttgc cgtgcgtttg gcataccttg aatcgccgcg tctgaaagcg
     4981 gttgcctgtc ttggtccggt agttcatacc ctgttgagtg attttaagtg ccagcaacag
     5041 gtgccggaaa tgtatcttga cgttctggcg agtcgtttgg ggatgcatga tgcttccgac
     5101 gaagcgttac gcgtggagct gaatcgctat tcattaaaag tgcaaggatt gctggggcgt
     5161 cgctgcccaa cgccaatgtt atcaggctac tggaagaacg atccgttcag cccggaagag
     5221 gactcacgct taatcacctc atcatctgct gacggtaaat tattagagat cccatttaac
     5281 ccggtgtatc ggaattttga caaaggtctt caggaaatca ccggctggat cgaaaaacgc
     5341 ttgtgttaaa aatttgctaa attttgccaa tttggtaaaa cagttgcatc acaacaggag
     5401 atagcaatga cgttaccgag tggacacccg aagagcagat tgatcaaaaa atttaccgca
     5461 ctaggcccgt atattcgtga aggtaagtgc gaagataatc gattcttttt cgattgtctg
     5521 gctgtatgcg tcaacgtgaa accggcaccg gaagtgcgtg aattctgggg ctggtggatg
     5581 gagctggaag cgcaggaatc ccgttttacc tacagctacc agtttggtct gttcgataaa
     5641 gcaggcgact ggaagagtgt tccggtaaaa gatactgaag tggttgaacg actggagcac
     5701 accctgcgtg agtttcacga gaagctgcgt gaactgctga cgacgctgaa tctgaagctg
     5761 gaaccggcgg atgattttcg tgatgaaccg gtgaagttaa cggcgtgagt gaaatgtgcc
     5821 ggatgcatca catccggcaa tattcattaa aactgatacg tcatcccaac cgcgacagta
     5881 tcatcattat taatattcag cttattatcg ctatccagtt tgttgatttt ataatcaaca
     5941 aacgctgaca tatttttgtt gaaataatac gtagcaccga cgtcgatata attgaccaga
     6001 tcttcatcac cgatgccctc aatatctttc cctttcgata agacataacc cagcgatgga
     6061 cgcagaccaa agtcaaactg gtattgagcg accgcttcaa agttctgtgt cttattggca
     6121 aagccgccag ttattggcgt cattttgcgt gtttcagaat agaaagttgc cagataaata
     6181 ttattggcat cgtatttcag acctgttgcc catgcttctg cacgcttgcc tgtgccacgg
     6241 ctttgcaggt tctgctcgtt ggtgcgatct gagttggtat aggccccact aatggcgaaa
     6301 tcgctgccgc caaagtcata tgtcaatgac gtgccgaagc catcgccgtt ttgcttttta
     6361 acgtcgcggt tttcgttttt cccttgatat tgcagggtta agttcaggcc atcgataacg
     6421 ccgaagaagt cggtgttccg atacgtcgcc agaccgctgg cgcgtttggt cataaagttg
     6481 tcggtctgcg ccgaggaatc gccaccaaat tccgggaaca tatcggtcca ggcttccacg
     6541 tcatacaacg cgcccaggtt acgaccataa tcgaaagaac ccaaatcttt atatttcaac
     6601 ccggcaaaag cgagacgcgt tttttgctgt gcagtatcac tctccgcttt atttccggca
     6661 aactccgctt cccaccgacc ataaccagtc agttgatcgt taatttgagt ttcaccttta
     6721 aaaccaaaac ggatataact ctggtcgcca tctttactgg cgttatcact catataatgc
     6781 atggctttaa ctttgccata gacatccagt ttattaccgt ctttattata tatttctgca
     6841 gcctgtacag atgcagatgc cacaatgccc attaccacta atgccagagt gctctttttc
     6901 attttcattc ctgattttaa ttaacgcgcg aatattcagc gggagagccc cgttgaaaac
     6961 aggaaagttt ttaacctgag attgttaaag atatattaca gattaatgat attctgaaaa
     7021 tgtggtaatt tattaaatct gtaataaaag cgtaaacaac tgccgctacg cttgctgatc
     7081 ccgcgcaaca aaacgccatg ctttgctcgc agatggttgg caaccgacga cagtcctgct
     7141 aaaacgttcg tttgatatca tttttcctaa aattgaatgg cagagaatca tgagtgacag
     7201 ccagacgctg gtggtaaaac tcggcaccag tgtgctaaca ggcggatcgc gccgtctgaa
     7261 ccgtgcccat atcgttgaac ttgttcgcca gtgcgcgcag ttacatgccg ccgggcatcg
     7321 gattgttatt gtgacgtcgg gcgcgatcgc cgccggacgt gagcacctgg gttacccgga
     7381 actgccagcg actatcgcct cgaaacaact gctggcggcg gtagggcaga gtcgactgat
     7441 tcaactgtgg gaacagctgt tttcgattta tggcattcac gtcgggcaaa tgctgctgac
     7501 ccgtgctgat atggaagacc gtgaacgctt cctgaacgcc cgcgacaccc tgcgtgcgtt
     7561 gctcgatcac aatatcgttc cggtaatcaa tgagaacgat gctgtcgcta cggcagagat
     7621 taaggtcggc gataacgata acctttctgc gctggcggcg attctggcgg gtgccgataa
     7681 actgttgctg ctgaccgatc aaaaaggttt gtataccgct gatccgcgca gcaatccgca
     7741 ggcagaactg attaaagatg tttacggcat tgatgacgca ctgcgcgcga ttgccggtga
     7801 cagcgtttca ggcctcggaa ctggcggcat gagtactaaa ttgcaggccg ctgacgtggc
     7861 ttgccgtgcg ggtatcgaca ccattattgc cgcgggcagc aagccgggcg ttattggtga
     7921 tgtgatggaa ggcatttccg tcggtacgct gttccatgcc caggcgactc cgcttgaaaa
     7981 ccgtaaacgc tggattttcg gtgcgccgcc ggcgggtgaa atcacggtag atgaaggggc
     8041 aactgccgcc attctggaac gcggcagctc cctgttgccg aaaggcatta aaagcgtgac
     8101 tggcaatttc tcgcgtggtg aagtcatccg catttgcaac ctcgaaggtc gcgatatcgc
     8161 ccacggtgtc agtcgttaca acagcgatgc attacgccgt attgccggac accactcgca
     8221 agaaattgat gcaatactgg gatatgaata cggcccggtt gccgttcacc gtgatgacat
     8281 gattacccgt taaggagctg gccgatgctg gaacaaatgg gcattgccgc gaagcaagcc
     8341 tcgtataaat tagcgcaact ctccagccgc gaaaaaaatc gcgtgctgga aaaaatcgcc
     8401 gatgaactgg aagcacaaag cgaaatcatc ctcaacgcta acgcccagga tgttgctgac
     8461 gcgcgagcca atggccttag cgaagcgatg cttgaccgtc tggcactgac gcccgcacgg
     8521 ctgaaaggca ttgccgacga tgtacgtcag gtgtgcaacc tcgccgatcc ggtggggcag
     8581 gtaatcgatg gcggcgtact ggacagcggc ctgcgtcttg agcgtcgtcg cgtaccgctg
     8641 ggggttattg gcgtgattta tgaagcgcgc ccgaacgtga cggttgatgt cgcttcgctg
     8701 tgcctgaaaa ccggtaatgc ggtgatcctg cgtggtggta aagaaacctg tcgcactaac
     8761 gcggcaacgg tggcggtgat tcaggacgcc ctgaaatcct gtggcttacc ggcgggtgcc
     8821 gtgcaggcga ttgataatcc tgaccgtgcg ctggtcagtg aaatgctgcg tatggataaa
     8881 tacatcgaca tgctgatccc gcgtggtggc gctggtttgc ataaactgtg ccgtgaacag
     8941 tcgacaatcc cggtgatcac aggtggtata ggcgtatgcc atatttacgt tgatgaaagc
     9001 gcagagatcg ctgaagcatt aaaagtgatc gtcaacgcga aaactcagcg tccgagcaca
     9061 tgtaatacgg ttgaaacgtt gctggtgaat aaaaacatcg ccgatagctt cctgcccgca
     9121 ttaagcaaac aaatggcgga aagcggcgtg acattacacg cagatgcagc tgcgctgaca
     9181 cagttgcagg caggccctgc gaaggtggtg gcggttaaag ccgaagagta tgacgatgag
     9241 tttctgtcat tagatttgaa cgtcaaaatc gtcagcgatc ttgacgatgc catcgcccat
     9301 attcgtgaac acggcacaca acactccgat gcgatcctga cccgcgatat gcgcaacgcc
     9361 cagcgttttg ttaacgaagt ggattcgtcc gctgtttacg ttaacgcctc tacgcgtttt
     9421 accgacggcg gccagtttgg tctgggtgcg gaagtggcgg taagcacaca aaaactccac
     9481 gcgcgtggcc caatggggct ggaagcactg accacttaca agtggatcgg cattggtgat
     9541 tacaccattc gtgcgtaaat aaaaccgggt gatgcaaaag tagccatttg attcacaagg
     9601 ccattgacgc atcgcccggt tagttttaac cttgtccacc gtgattcacg ttcgtgaaca
     9661 tgtcctttca gggccgatat agctcagttg gtagagcagc gcattcgtaa tgcgaaggtc
     9721 gtaggttcga ctcctattat cggcaccatt taaatcaata agttacctcg catttaagta
     9781 aaccacgttc tcctcttgtg ccgtatttgt gccattgcga cttataatcg catcgatttt
     9841 gcttgcgtgc tcggtgagat gcccggctga aaggtgggcg tatctttgaa ccatttcgag
     9901 agtttcccat cctcccatct ctttaagtgc aagaagagag acaccggact gaaccagcca
     9961 gcttgcccag gtatgcctca gatcatggaa gcggaagttg ctaatgcctg cccgctttaa
    10021 cgctcccttc catgccttgt tgctgtcggt tctcatcttc cttaccgctg ctgtttttgt
    10081 tccgtcgctt cggtaggcag gtttggtgtg gacaaatacc catctcttat ggagcccctg
    10141 ctgttttctt aatatctggc atgcggtttc gttaagagga actccgatcg cattgccagc
    10201 ttttgtttca tcagggtgca tccatgccat tttcttatcc agatcgacct gtgaccactc
    10261 aaggtctgta acgttggaac ggcgaaggcc tgtcgtgatt gcaaacatga ccacagggaa
    10321 gaagtgagga gcaatttctg caaacaggcg cttcgattcc tcctctgtaa gccatctgat
    10381 gcgtccattc ttaacgcgtg gtgttgatat tttgggtgcc ctgtcaagcc atccccattc
    10441 aacagccata ttgagaatgg cgcgaagtat tgccagatgc ctcgtcttcg ttcctttgct
    10501 tgcaagcttt ggtttatact ccggcaccgg attgccaagt cgcaaacacc tgtcccggct
    10561 catctcccag ttcaggcgat ggcggcggtt ttccatcccg tctaccgcct ccattatttt
    10621 ttctgttgtt atgtcagaga gaatggcttc tctgaagtgc aacatccaga acgatataat
    10681 gctcttgtca tcatcaatgg acttcttatc cgatttctca cgcagccacc gtatgcaggc
    10741 ttccttgaat agctttttcg gtgattcccc gagatttttt actctccacg ctttgaagtg
    10801 gtcaacaaaa actggccacc gagttagagt
//
LOCUS       DAETPZ010000338         7710 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.guided.145, whole genome shotgun sequence.
ACCESSION   DAETPZ010000338 DAETPZ010000000
VERSION     DAETPZ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7710
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.guided.145"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..359)
                     /locus_tag="K0W59_004500"
     CDS             complement(<1..359)
                     /locus_tag="K0W59_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI3025165.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLS"
     gene            complement(426..3392)
                     /locus_tag="K0W59_004501"
     CDS             complement(426..3392)
                     /locus_tag="K0W59_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HBI3025166.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(3395..3955)
                     /locus_tag="K0W59_004502"
     CDS             complement(3395..3955)
                     /locus_tag="K0W59_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI3025167.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(4081..4431)
                     /locus_tag="K0W59_004503"
     CDS             complement(4081..4431)
                     /locus_tag="K0W59_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HBI3025168.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(4634..5647)
                     /gene="intI1"
                     /locus_tag="K0W59_004504"
     CDS             complement(4634..5647)
                     /gene="intI1"
                     /locus_tag="K0W59_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HBI3025169.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            5857..6687
                     /locus_tag="K0W59_004505"
     CDS             5857..6687
                     /locus_tag="K0W59_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HBI3025170.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            6800..7591
                     /gene="aadA1"
                     /locus_tag="K0W59_004506"
     CDS             6800..7591
                     /gene="aadA1"
                     /locus_tag="K0W59_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HBI3025171.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1706 a         2120 c         2229 g         1655 t
ORIGIN      
        1 ttgctcaggc tctccccgtg gaggtaataa ttgacgatat gatcatttat tctgcctccc
       61 agagcctgat aaaaacggtt agcgccgggg ttggattttt cagcgttcca gctaaggcta
      121 aggcattcct gttcaagcgc aagccgggct ataaagcgca ttatcgcttt acccgtgccc
      181 ttatttcgat cgcactgaga aacatacagc tctttaatat gcagctgacc gctgtatcgg
      241 ggcgagggat aaagaatatt gcaacatgcc aggccagtaa tgttattgcc gcagcgcgct
      301 ctgatcacca gggtaccgga aagccggttg aataactttt tacagagata atccttcatc
      361 aacgcttcct gaatgatacc ttcgccataa taggggtcgt ctcagaaaac ggaaaataaa
      421 gcacgctaag ccggttgcag aggccgtagc ggcctgaact tccccgcgcc gatcttggcg
      481 ctgctgcgcc ataggtaatc accggtcagg ttgatgtgct cccagccgag tggcgacagg
      541 tactgcaata gcgagtcatc gacggcatga ccattgccgc gcaacgcatg cgccgcacgc
      601 tccaggtaga ccgtgttcca cagcacgatg gccgccgtca ccaggttgag gccgctggcc
      661 cggtagcgct gctgctcgaa actgcggtca cggatttcac caaggcggtt gaagaacacg
      721 gcacgggcca gcgcattgcg cgcctcgccc ttgttcagcc cggcatgcac gcggcggcgt
      781 agctcgacgc tttgcagcca gtcgaggatg aacagcgtgc gctcgatgcg gcccaactcg
      841 cgcagcgcga cggccaagcc gttctggcgc gggtagctgc cgagtttcct gagcatcagc
      901 gaggccgtca ccgtgccctg cttgatcgag gtggccagcc gcaggatttc gtcccaatgg
      961 gcgcggacgt gcttgatgtt gagcgtgccg ccgatcatcg gcttgagcgc gtcataggcg
     1021 gcatcgccct tcgggatgta gagcttggtg tcgcccaggt cgcggatgcg cggcgcgaag
     1081 cggaagccca agaggtgcat cagggcgaag acgtgatcgg tgaagcccgc cgtgtcggtg
     1141 tagtgctcct cgatccgcag gtcggattcg tggtacagca ggccgtcgag cacgtaggtt
     1201 gagtcgcgca ggccgacatt gaccaccttg gtgtggaatg gcgcgtattg gtcggagatg
     1261 tgggtgtaga aagtccgtcc tgggctgctg ccatattttg ggttgatgtg ccccgtgctc
     1321 tttgccttgc tagcggttcg gaaattctgt ccgtccgatg atgatgtggt gccatcgccc
     1381 cagtgcccgg caaagggatg ccgaaactga gcgttgacca gttcagccaa cgctgtcgag
     1441 tacgtttcgt cgcgggtatg ccaggcttgc agccaagcga gcttcgcgta ggtcgtgccg
     1501 gggcaggact cggccatctt ggtcaggccc aggttgatcg cgtcggccag gatcgtggtc
     1561 aacaacaggt tcttgtcctt ggccagatcg cccgatttca agtgcgtgaa gtgccgggtg
     1621 aagcccgtcc actcatcgac ttcgagcagc agttcggtga tcttgacgtg cggcaggacc
     1681 atggctgtct ggtctatcag cgcctgcgcg gtgtcgggca ccgccgcatc cagcggcgtg
     1741 atcttcaagc ccgactcggt gatgatggca tccggcaggt cgttggctgc cgccatgcgg
     1801 ttgacggtgg caagttgtgc ttccagcagc gtcagccgct catgcagata ttgttcgcag
     1861 tcggtggcca cggccagcgg caattcgctg gactgcttga ggctggtgaa cttctcgggc
     1921 ggtaccaggt agtcctcgaa gtccttgaac tggcgtgaac cctgcaccca gatgtcgccc
     1981 gagcgcaggg agttcttcaa ctcggacagc gcgcacagtt cgtagtagcg ccggtcgatg
     2041 ccggcgtcgg tcatcaccag tttctgccag cgcggcttga tgaagccggt cggtgcatcg
     2101 gctggcagct tgcgggcgtt gtcggtgttc atgccgcgca gcacctcaat ggcatcaagc
     2161 acgtttttgg cggcgggcgc ggcccgcagc ttgagcacgg caaggaattc cggtgcatag
     2221 cggcgcaggg tggcgtagct ctcgccgatg cgatgcagga aatcgaagtc atcgggttgc
     2281 gcgagcttct gcgcctcggt gacgctctcg gcaaaggaat cccaggacat gacggcctcg
     2341 atggcggcaa acgcatcgcg gcctgattgc ttggcgtcga tcagcgcctg accgatgcgc
     2401 ccgtacagac gtaccttggc gttgatggcc ttgcctgacg cctggaactg ctgctgatgc
     2461 ttattcttgg cagcgttaaa cagcttaccc aggatgcggt cgtgcaggtc gatgatttcg
     2521 tcggtgacgg tggccatgcc ctcggtggcc agcgccacga gagtggcgta gcgccgttgc
     2581 ggctcgaatt tggccaggtc ggcgggtgtc atctggccgc cctcgcgggc aatcttgagc
     2641 aggcggttct ggtgaaccag ccgctcgatg ccggtaggca gatcgagtgc ctgccatgcc
     2701 ttgaggcgtt cgatgtgttc cagcatatgc cgcgaatttg gcttggccgg agactggcgc
     2761 aaccaagcca accaggtcgt cttgccgttg tcccggcgct tgagcagatc gtcgaggcgg
     2821 cggcgatgcg cgtccgccag tggttcggcc aaggcgtcgt agatgcgccg gttagcacgg
     2881 gtgatcgcct cggcactcgc ccgctcgacg gcgttgaggg cgggcagaat gaccgactgc
     2941 cgccgcaggt gcccgatcaa ggcgctggcc agcacgatgc ctttgtcggt ttgcatcgcc
     3001 agctcggtca gcatctggac ggcctgccgg taatggctca tggtgaaggg ccggaaaccg
     3061 aacacggttt gcagctcgct caggtgctcg cgccgggtct gctcccgctg gccgtactcg
     3121 ttccagcttt cgacgccgac cttgagctgg tcggcgacca gcttcaacaa gggcgggaac
     3181 ggtagttcat cgacgcccag gatgacgccg ggaaagcgca ggtaacagag ctgcaccgcg
     3241 aagcccagcc gattggctgg cccgcgccgc tgtcggatga tcgagaggtc ggtatcgttg
     3301 aatgtgtaat gtcggatcag gtcgtccttg gagtccggca acgccagcag gctttcccgc
     3361 tcggcggcgg acaggatgga acgacgtggc atatttactg atccgttctc aagtattgat
     3421 acagggtttc gcgactgatt ccgaattcac gagcaagctt ggtcttttgc tcgccagcct
     3481 cgacacgttg gcgcagttcg gcaatacgct cagacgacag ggatttcttc ctgccacggt
     3541 aagccccgcg ttgcttggcg agcgcaatac cctcgcgctg acgctcgcgg atcagggcgc
     3601 gctcgaactc ggcgaacgcg cccatcaccg agagcatcag gttcgccatc ggagagtctt
     3661 cgccagtaaa actgaggtgt tccttgacga attcgatatg cacgccgcgt tgtgtcagcg
     3721 tttgcacgat ccggcgcaaa tcatcgagat tgcgcgccag gcgatccatg ctatgcacca
     3781 ccacggtgtc gccggtgcgg gcgaagctta tcagcgcttc cagttgcgga cgcttgacat
     3841 ccttgccgga tgccttgtcg ctaaaagcgc gatcaacctt gacgccttcc agttgccgtt
     3901 ccgggttctg gtcgaaggtg ctgaccctga tatacccaat gcgctgtcca gtcatggaat
     3961 tccctgcaaa atgtcaggga agactctatg accttcaacg agatatgtca ataaattcaa
     4021 aattcaatcc tatcctgacg caatttacac atggcatctg acatcaggtt agggtatgcc
     4081 tcaacctgac ggcggcgaat cacaagcgtc cggtttgacg ctggtttcgg tcgcagtgct
     4141 ggcccgcgcc tggaagcgct gatagcactc cagcccgcag aaatgctcca cgtactcggc
     4201 cccttccggc gtgaaggcgg catcgagcgg gatttccttg cagcacacgc agcaactggt
     4261 gctcggttca ttggcgttca tggtggtgtt cctccattgg ttgacgaagc cgacgaaggc
     4321 cgccgccggc atcggcctgg cgaacaggaa accctgcacc gtgtcgcaac ccgcctgccg
     4381 caaccacgca aggcagtcgg gtgtttccac accctcggct accacctcca ttgtcgtttt
     4441 cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg aggggttgga
     4501 tccatcaggc aacgacgggc tgctgccggc catcagcgga cgcagggagg actttccgca
     4561 accggccgtt cgatgcggca ccgatggcct tcgcgcaggg gtagtgaatc cgccaggatt
     4621 gacttgcgct gccctacctc tcactagtga ggggcggcag cgcatcaagc ggtgagcgca
     4681 ctccggcacc gccaactttc agcacatgcg tgtaaatcat cgtcgtagag acgtcggaat
     4741 ggccgagcag atcctgcacg gttcgaatgt cgtaaccgct gcggagcaag gccgtcgcga
     4801 acgagtggcg gagggtgtgc ggtgtggcgg gcttcgtgat gcctgcttgt tctacggcac
     4861 gtttgaaggc gcgctgaaag gtctggtcat acatgtgatg gcgacgcacg acaccgctcc
     4921 gtggatcggt cgaatgcgtg tgctgcgcaa aaacccagaa ccacggccag gaatgcccgg
     4981 cgcgcggata cttccgctca agggcgtcgg gaagcgcaac gccgctgcgg ccctcggcct
     5041 ggtccttcag ccaccatgcc cgtgcacgcg acagctgctc gcgcaggctg ggtgccaagc
     5101 tctcgggtaa catcaaggcc cgatccttgg agcccttgcc ctcccgcacg atgatcgtgc
     5161 cgtgatcgaa atccagatcc ttgacccgca gttgcaaacc ctcactgatc cgcatgcccg
     5221 ttccatacag aagctgggcg aacaaacgat gctcgccttc cagaaaaccg aggatgcgaa
     5281 ccacttcatc cggggtcagc accaccggca agcgccgcga cggccgaggt cttccgatct
     5341 cctgaagcca gggcagatcc gtgcacagca ccttgccgta gaagaacagc aaggccgcca
     5401 atgcctgacg atgcgtggag accgaaacct tgcgctcgtt cgccagccag gacagaaatg
     5461 cctcgacttc gctgctgccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg
     5521 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     5581 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     5641 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     5701 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     5761 agcagggcag tcgccctaaa acaaagttgg gcgaacccgg agcctcatta attgttagcc
     5821 gttaaaatta agccctttac caaaccaata cttattatga aaaacacaat acatatcaac
     5881 ttcgctattt ttttaataat tgcaaatatt atctacagca gcgccagtgc atcaacagat
     5941 atctctactg ttgcatctcc attatttgaa ggaactgaag gttgtttttt actttacgat
     6001 gcatccacaa acgctgaaat tgctcaattc aataaagcaa agtgtgcaac gcaaatggca
     6061 ccagattcaa ctttcaagat cgcattatca cttatggcat ttgatgcgga aataatagat
     6121 cagaaaacca tattcaaatg ggataaaacc cccaaaggaa tggagatctg gaacagcaat
     6181 catacaccaa agacgtggat gcaattttct gttgtttggg tttcgcaaga aataacccaa
     6241 aaaattggat taaataaaat caagaattat ctcaaagatt ttgattatgg aaatcaagac
     6301 ttctctggag ataaagaaag aaacaacgga ttaacagaag catggctcga aagtagctta
     6361 aaaatttcac cagaagaaca aattcaattc ctgcgtaaaa ttattaatca caatctccca
     6421 gttaaaaact cagccataga aaacaccata gagaacatgt atctacaaga tctggataat
     6481 agtacaaaac tgtatgggaa aactggtgca ggattcacag caaatagaac cttacaaaac
     6541 ggatggtttg aagggtttat tataagcaaa tcaggacata aatatgtttt tgtgtccgca
     6601 cttacaggaa acttggggtc gaatttaaca tcaagcataa aagccaagaa aaatgcgatc
     6661 accattctaa acacactaaa tttataaaaa atctaatggc aaaatcgccc aacccttcaa
     6721 tcaagtcggg acggccaaaa gcaagctttt ggctcccctc gctggcgctc ggcgcccctt
     6781 atttcaaacg ttaaacatca tgagggaagt ggtgatcgcc gaagtatcga ctcaactatc
     6841 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     6901 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     6961 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     7021 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     7081 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     7141 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     7201 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     7261 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     7321 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     7381 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     7441 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     7501 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgttc actacgtgaa
     7561 aggcgagatc accaaggtag tcggcaaata atgtctaaca attcgttcaa gccgacgccg
     7621 cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg cggtgatgct
     7681 gccaacttac tgatttagtg tatgatggtg
//
LOCUS       DAETPZ010000339         4853 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.guided.188, whole genome shotgun sequence.
ACCESSION   DAETPZ010000339 DAETPZ010000000
VERSION     DAETPZ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.guided.188"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            142..546
                     /locus_tag="K0W59_004507"
     CDS             142..546
                     /locus_tag="K0W59_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBI3025172.1"
                     /translation="MGISLNHGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQ
                     QWITENGSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSL
                     YESKGWLTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(524..1147)
                     /gene="tetR(B)"
                     /locus_tag="K0W59_004508"
     CDS             complement(524..1147)
                     /gene="tetR(B)"
                     /locus_tag="K0W59_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HBI3025173.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1229..2434
                     /gene="tet(B)"
                     /locus_tag="K0W59_004509"
     CDS             1229..2434
                     /gene="tet(B)"
                     /locus_tag="K0W59_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HBI3025174.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMAGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2547..3140)
                     /gene="tetC"
                     /locus_tag="K0W59_004510"
     CDS             complement(2547..3140)
                     /gene="tetC"
                     /locus_tag="K0W59_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HBI3025175.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3273..3644
                     /locus_tag="K0W59_004511"
     CDS             3273..3644
                     /locus_tag="K0W59_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3025176.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(3654..>4853)
                     /locus_tag="K0W59_004512"
     CDS             complement(3654..>4853)
                     /locus_tag="K0W59_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HBI3025177.1"
                     /translation="EHKTLSVSWSHYPELHLKRLNSLTLACHALLDCKTLTLTELGRN
                     LPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQK
                     RLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAG
                     FKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTK
                     SNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPK
                     QLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGV
                     HAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTH
                     GYALGKL"
BASE COUNT         1299 a          918 c         1044 g         1592 t
ORIGIN      
        1 gtggcgaata tatcagcttc aacggcgagc agtcatttat ctaaattatt agattgccag
       61 ctaatcacag tagtagctca aggcaagcat cgttattttc ggctagcagg aaaagatatt
      121 gctgaattga tggaaagtat gatggggatc tccttaaacc atggcgtaca tgccaaagtt
      181 tccacgccag tgcatttacg aaaagcacgt acttgctatg atcatttagc tggcgaagtt
      241 gccgttaaga tctatgattc cctttgtcaa cagcaatgga tcactgaaaa tggttcaatg
      301 atcacattaa gtggtattca atattttcat gaaatgggaa ttgacgttcc ttccaaacat
      361 tcacgtaaaa tctgttgtgc gtgtttagat tggagtgaac gccgtttcca tttaggtggg
      421 tacgttggag ccgcattatt ttcgctttat gaatctaaag ggtggttaac tcgacatctt
      481 ggttaccgtg aagttaccat cacggaaaaa ggttatgctg cttttaagac ccactttcac
      541 atttaagttg tttttctaat ccgcatatga tcaattcaag gccgaataag aaggctggct
      601 ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa taatggcggc atactatcag
      661 tagtaggtgt ttccctttct tctttagcga cttgatgctc ttgatcttcc aatacgcaac
      721 ctaaagtaaa atgccccaca gcgctgagtg catataatgc attctctagt gaaaaacctt
      781 gttggcataa aaaggctaat tgattttcga gagtttcata ctgtttttct gtaggccgtg
      841 tacctaaatg tacttttgct ccatcgcgat gacttagtaa agcacatcta aaacttttag
      901 cgttattacg taaaaaatct tgccagcttt ccccttctaa agggcaaaag tgagtatggt
      961 gcctatctaa catctcaatg gctaaggcgt cgagcaaagc ccgcttattt tttacatgcc
     1021 aatacaatgt aggctgctct acacctagct tctgggcgag tttacgggtt gttaaacctt
     1081 cgattccgac ctcattaagc agctctaatg cgctgttaat cactttactt ttatctaatc
     1141 tagacatcat taattcctaa tttttgttga cactctatca ttgatagagt tattttacca
     1201 ctccctatca gtgatagaga aaagtgaaat gaatagttcg acaaagatcg cattggtaat
     1261 tacgttactc gatgccatgg ggattggcct tatcatgcca gtcttgccaa cgttattacg
     1321 tgaatttatt gcttcggaag atatcgctaa ccactttggc gtattgcttg cactttatgc
     1381 gttaatgcag gttatctttg ctccttggct tggaaaaatg tctgaccgat ttggtcggcg
     1441 cccagtgctg ttgttgtcat taataggcgc atcgctggat tacttattgc tggctttttc
     1501 aagtgcgctt tggatgctgt atttaggccg tttgctttca gggatcacag gagctactgg
     1561 ggctgtcgcg gcatcggtca ttgccgatac cacctcagct tctcaacgcg tgaagtggtt
     1621 cggttggtta ggggcaagtt ttgggcttgg tttaatagcg gggcctatta ttggtggttt
     1681 tgcaggagag atttcaccgc atagtccctt ttttatcgct gcgttgctaa atattgtcgc
     1741 tttccttgtg gttatgtttt ggttccgtga aaccaaaaat acacgtgata atacagatac
     1801 cgaagtaggg gttgagacgc aatcgaattc ggtatacatc actttattta aaacgatgcc
     1861 cattttgttg attatttatt tttcagcgca attgataggc caaattcccg caacggtgtg
     1921 ggtgctattt accgaaaatc gttttggatg gaatagcatg atggctggct tttcattagc
     1981 gggtcttggt cttttacact cagtattcca agcctttgtg gcaggaagaa tagccactaa
     2041 atggggcgaa aaaacggcag tactgctcgg atttattgca gatagtagtg catttgcctt
     2101 tttagcgttt atatctgaag gttggttagt tttccctgtt ttaattttat tggctggtgg
     2161 tgggatcgct ttacctgcat tacagggagt gatgtctatc caaacaaaga gtcatcagca
     2221 aggtgcttta cagggattat tggtgagcct taccaatgca accggtgtta ttggcccatt
     2281 actgtttgct gttatttata atcattcact accaatttgg gatggctgga tttggattat
     2341 tggtttagcg ttttactgta ttattatcct gctatcgatg accttcatgt taacccctca
     2401 agctcagggg agtaaacagg agacaagtgc ttagttattt cgtcaccaaa tgatgttatt
     2461 ccgcgaaata taatgaccct cttgataacc caagagggca ttttttacga taaagaagat
     2521 ttagcttcaa ataaaaccta tctattttat ttatctttca agctcaataa aaagccgcgg
     2581 taaatagcaa taaattggcc ttttttatcg gcaagctctt ttaggttttt cgcatgtatt
     2641 gcgatatgca taaaccagcc attgagtaag tttttaagca catcatcatc ataagcttta
     2701 agttggttct cttggatcaa tttgctgaca atggcgttta ccttaccagt aatgtattca
     2761 aggctaattt tttcaagttc attccaacca atgataggca tcacttcttg gatagggata
     2821 aggtttttat tattatcaat aatataatca agataatgtt caaatatact ttctaaggca
     2881 gaccaaccat ttgttaaatc agtttttgtt gtgatgtagg catcaatcat aattaattgc
     2941 tgcttataac aggcactgag taattgtttt ttatttttaa agtgatgata aaaggcacct
     3001 ttggtcacca acgcttttcc cgagatctca tctattgaaa cagcttgata gcctttttca
     3061 acaaacaata ttcgtgctga gttaaccagt gattgatagg tactcttaaa attttcttgt
     3121 tgatgatttt tattttccat gatagattta aaataacata ccgtcagtat gtttatggta
     3181 tcatgatgat gtggtcgtga caatcttaag aacatttagg ttattttatg tatattgaac
     3241 agcattctcg ctatcaaaat aaagctaata acatccaatt aagatatgat gataagcagt
     3301 ttcatacaac ggttatcaaa gatgttctat tatggattga acataattta gatcagtctt
     3361 tactgcttga tgatgtggcg aataaagcgg gttataccaa gtggtatttt cagcggctgt
     3421 tcaaaaaagt aacaggggtc acactggcta gctatattcg tgctcgtcgt ttgacgaaag
     3481 cggctgttga gttgaggttg acgaaaaaaa ctatccttga gatcgcatta aaatatcaat
     3541 ttgattccca acaatctttt acacgtcgat ttaagtacat ttttaaggtt acaccaagtt
     3601 attatcggcg taataaatta tgggaattgg aggcaatgca ctgagagatc ccctcataat
     3661 ttccccaaag cgtaaccatg tgtgaataaa ttttgagcta gtagggttgc agccacgagt
     3721 aagtcttccc ttgttattgt gtagccagaa tgccgcaaaa cttccatgcc taagcgaact
     3781 gttgagagta cgtttcgatt tctgactgtg ttagcctgga agtgcttgtc ccaaccttgt
     3841 ttctgagcat gaacgcccgc aagccaacat gttagttgaa gcatcagggc gattagcagc
     3901 atgatatcaa aacgctctga gctgctcgtt cggctatggc gtaggcctag tccgtaggca
     3961 ggacttttca agtctcggaa ggtttcttca atctgcattc gcttcgaata gatattaaca
     4021 agttgtttgg gtgttcgaat ttcaacaggt aagttagttg ctagaaccca tggctccttt
     4081 gccgacgctg agtagatttt aggtgacggg tggtgacaat gagtccgtgt cgagcgctga
     4141 ttttttcggc ctttagagcg agatttatac aatagaattt ggcatgagat tggattgctt
     4201 ttagtcagcc tcttatagcc taaagtcttt gagtgactag atgacatatc atgtaagttg
     4261 ctgataggtt tccagttttc cgctcctagg tctgcatatt gtacttttcc tcttactcga
     4321 cttaaccagt accaacccag cttctcaacg gatttatacc atggcacttt aaagccagca
     4381 tcactgacaa tgagcggtgt ggtgttactc ggtagaatgc tcgcaaggtc ggctagaaat
     4441 tggtcatgag ctttctttga acattgctct gaaagcggga acgctttctc ataaagagta
     4501 acagaacgac cgtgtagtgc gactgaagct cgcaatacca taagtcgttt ttgctcacga
     4561 atatcagacc agtcaacaag tacaatgggc atcgtattgc ccgaacagat aaagctagca
     4621 tgccaacggt atacagcgag tcgctctttg tggaggtgac gattacctaa caatcggtcg
     4681 attcgtttga tgttatgttt tgttctcgct ttggttggca ggttacggcc aagttcggta
     4741 agagtgagag ttttacagtc aagtaatgcg tggcaagcca acgttaagct gttgagtcgt
     4801 tttaagtgta attcggggta atgactccaa cttactgata gtgttttatg ttc
//
LOCUS       DAETPZ010000340         4853 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.guided.189, whole genome shotgun sequence.
ACCESSION   DAETPZ010000340 DAETPZ010000000
VERSION     DAETPZ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.guided.189"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            142..546
                     /locus_tag="K0W59_004513"
     CDS             142..546
                     /locus_tag="K0W59_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBI3025178.1"
                     /translation="MGISLNHGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQ
                     QWITENGSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSL
                     YESKGWLTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(524..1147)
                     /gene="tetR(B)"
                     /locus_tag="K0W59_004514"
     CDS             complement(524..1147)
                     /gene="tetR(B)"
                     /locus_tag="K0W59_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HBI3025179.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1229..2434
                     /gene="tet(B)"
                     /locus_tag="K0W59_004515"
     CDS             1229..2434
                     /gene="tet(B)"
                     /locus_tag="K0W59_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HBI3025180.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2547..3140)
                     /gene="tetC"
                     /locus_tag="K0W59_004516"
     CDS             complement(2547..3140)
                     /gene="tetC"
                     /locus_tag="K0W59_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HBI3025181.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3273..3644
                     /locus_tag="K0W59_004517"
     CDS             3273..3644
                     /locus_tag="K0W59_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3025182.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(3654..>4853)
                     /locus_tag="K0W59_004518"
     CDS             complement(3654..>4853)
                     /locus_tag="K0W59_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HBI3025183.1"
                     /translation="EHKTLSVSWSHYPELHLKRLNSLTLACHALLDCKTLTLTELGRN
                     LPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQK
                     RLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAG
                     FKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTK
                     SNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPK
                     QLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGV
                     HAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTH
                     GYALGKL"
BASE COUNT         1299 a          917 c         1044 g         1593 t
ORIGIN      
        1 gtggcgaata tatcagcttc aacggcgagc agtcatttat ctaaattatt agattgccag
       61 ctaatcacag tagtagctca aggcaagcat cgttattttc ggctagcagg aaaagatatt
      121 gctgaattga tggaaagtat gatggggatc tccttaaacc atggcgtaca tgccaaagtt
      181 tccacgccag tgcatttacg aaaagcacgt acttgctatg atcatttagc tggcgaagtt
      241 gccgttaaga tctatgattc cctttgtcaa cagcaatgga tcactgaaaa tggttcaatg
      301 atcacattaa gtggtattca atattttcat gaaatgggaa ttgacgttcc ttccaaacat
      361 tcacgtaaaa tctgttgtgc gtgtttagat tggagtgaac gccgtttcca tttaggtggg
      421 tacgttggag ccgcattatt ttcgctttat gaatctaaag ggtggttaac tcgacatctt
      481 ggttaccgtg aagttaccat cacggaaaaa ggttatgctg cttttaagac ccactttcac
      541 atttaagttg tttttctaat ccgcatatga tcaattcaag gccgaataag aaggctggct
      601 ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa taatggcggc atactatcag
      661 tagtaggtgt ttccctttct tctttagcga cttgatgctc ttgatcttcc aatacgcaac
      721 ctaaagtaaa atgccccaca gcgctgagtg catataatgc attctctagt gaaaaacctt
      781 gttggcataa aaaggctaat tgattttcga gagtttcata ctgtttttct gtaggccgtg
      841 tacctaaatg tacttttgct ccatcgcgat gacttagtaa agcacatcta aaacttttag
      901 cgttattacg taaaaaatct tgccagcttt ccccttctaa agggcaaaag tgagtatggt
      961 gcctatctaa catctcaatg gctaaggcgt cgagcaaagc ccgcttattt tttacatgcc
     1021 aatacaatgt aggctgctct acacctagct tctgggcgag tttacgggtt gttaaacctt
     1081 cgattccgac ctcattaagc agctctaatg cgctgttaat cactttactt ttatctaatc
     1141 tagacatcat taattcctaa tttttgttga cactctatca ttgatagagt tattttacca
     1201 ctccctatca gtgatagaga aaagtgaaat gaatagttcg acaaagatcg cattggtaat
     1261 tacgttactc gatgccatgg ggattggcct tatcatgcca gtcttgccaa cgttattacg
     1321 tgaatttatt gcttcggaag atatcgctaa ccactttggc gtattgcttg cactttatgc
     1381 gttaatgcag gttatctttg ctccttggct tggaaaaatg tctgaccgat ttggtcggcg
     1441 cccagtgctg ttgttgtcat taataggcgc atcgctggat tacttattgc tggctttttc
     1501 aagtgcgctt tggatgctgt atttaggccg tttgctttca gggatcacag gagctactgg
     1561 ggctgtcgcg gcatcggtca ttgccgatac cacctcagct tctcaacgcg tgaagtggtt
     1621 cggttggtta ggggcaagtt ttgggcttgg tttaatagcg gggcctatta ttggtggttt
     1681 tgcaggagag atttcaccgc atagtccctt ttttatcgct gcgttgctaa atattgtcgc
     1741 tttccttgtg gttatgtttt ggttccgtga aaccaaaaat acacgtgata atacagatac
     1801 cgaagtaggg gttgagacgc aatcgaattc ggtatacatc actttattta aaacgatgcc
     1861 cattttgttg attatttatt tttcagcgca attgataggc caaattcccg caacggtgtg
     1921 ggtgctattt accgaaaatc gttttggatg gaatagcatg atggttggct tttcattagc
     1981 gggtcttggt cttttacact cagtattcca agcctttgtg gcaggaagaa tagccactaa
     2041 atggggcgaa aaaacggcag tactgctcgg atttattgca gatagtagtg catttgcctt
     2101 tttagcgttt atatctgaag gttggttagt tttccctgtt ttaattttat tggctggtgg
     2161 tgggatcgct ttacctgcat tacagggagt gatgtctatc caaacaaaga gtcatcagca
     2221 aggtgcttta cagggattat tggtgagcct taccaatgca accggtgtta ttggcccatt
     2281 actgtttgct gttatttata atcattcact accaatttgg gatggctgga tttggattat
     2341 tggtttagcg ttttactgta ttattatcct gctatcgatg accttcatgt taacccctca
     2401 agctcagggg agtaaacagg agacaagtgc ttagttattt cgtcaccaaa tgatgttatt
     2461 ccgcgaaata taatgaccct cttgataacc caagagggca ttttttacga taaagaagat
     2521 ttagcttcaa ataaaaccta tctattttat ttatctttca agctcaataa aaagccgcgg
     2581 taaatagcaa taaattggcc ttttttatcg gcaagctctt ttaggttttt cgcatgtatt
     2641 gcgatatgca taaaccagcc attgagtaag tttttaagca catcatcatc ataagcttta
     2701 agttggttct cttggatcaa tttgctgaca atggcgttta ccttaccagt aatgtattca
     2761 aggctaattt tttcaagttc attccaacca atgataggca tcacttcttg gatagggata
     2821 aggtttttat tattatcaat aatataatca agataatgtt caaatatact ttctaaggca
     2881 gaccaaccat ttgttaaatc agtttttgtt gtgatgtagg catcaatcat aattaattgc
     2941 tgcttataac aggcactgag taattgtttt ttatttttaa agtgatgata aaaggcacct
     3001 ttggtcacca acgcttttcc cgagatctca tctattgaaa cagcttgata gcctttttca
     3061 acaaacaata ttcgtgctga gttaaccagt gattgatagg tactcttaaa attttcttgt
     3121 tgatgatttt tattttccat gatagattta aaataacata ccgtcagtat gtttatggta
     3181 tcatgatgat gtggtcgtga caatcttaag aacatttagg ttattttatg tatattgaac
     3241 agcattctcg ctatcaaaat aaagctaata acatccaatt aagatatgat gataagcagt
     3301 ttcatacaac ggttatcaaa gatgttctat tatggattga acataattta gatcagtctt
     3361 tactgcttga tgatgtggcg aataaagcgg gttataccaa gtggtatttt cagcggctgt
     3421 tcaaaaaagt aacaggggtc acactggcta gctatattcg tgctcgtcgt ttgacgaaag
     3481 cggctgttga gttgaggttg acgaaaaaaa ctatccttga gatcgcatta aaatatcaat
     3541 ttgattccca acaatctttt acacgtcgat ttaagtacat ttttaaggtt acaccaagtt
     3601 attatcggcg taataaatta tgggaattgg aggcaatgca ctgagagatc ccctcataat
     3661 ttccccaaag cgtaaccatg tgtgaataaa ttttgagcta gtagggttgc agccacgagt
     3721 aagtcttccc ttgttattgt gtagccagaa tgccgcaaaa cttccatgcc taagcgaact
     3781 gttgagagta cgtttcgatt tctgactgtg ttagcctgga agtgcttgtc ccaaccttgt
     3841 ttctgagcat gaacgcccgc aagccaacat gttagttgaa gcatcagggc gattagcagc
     3901 atgatatcaa aacgctctga gctgctcgtt cggctatggc gtaggcctag tccgtaggca
     3961 ggacttttca agtctcggaa ggtttcttca atctgcattc gcttcgaata gatattaaca
     4021 agttgtttgg gtgttcgaat ttcaacaggt aagttagttg ctagaaccca tggctccttt
     4081 gccgacgctg agtagatttt aggtgacggg tggtgacaat gagtccgtgt cgagcgctga
     4141 ttttttcggc ctttagagcg agatttatac aatagaattt ggcatgagat tggattgctt
     4201 ttagtcagcc tcttatagcc taaagtcttt gagtgactag atgacatatc atgtaagttg
     4261 ctgataggtt tccagttttc cgctcctagg tctgcatatt gtacttttcc tcttactcga
     4321 cttaaccagt accaacccag cttctcaacg gatttatacc atggcacttt aaagccagca
     4381 tcactgacaa tgagcggtgt ggtgttactc ggtagaatgc tcgcaaggtc ggctagaaat
     4441 tggtcatgag ctttctttga acattgctct gaaagcggga acgctttctc ataaagagta
     4501 acagaacgac cgtgtagtgc gactgaagct cgcaatacca taagtcgttt ttgctcacga
     4561 atatcagacc agtcaacaag tacaatgggc atcgtattgc ccgaacagat aaagctagca
     4621 tgccaacggt atacagcgag tcgctctttg tggaggtgac gattacctaa caatcggtcg
     4681 attcgtttga tgttatgttt tgttctcgct ttggttggca ggttacggcc aagttcggta
     4741 agagtgagag ttttacagtc aagtaatgcg tggcaagcca acgttaagct gttgagtcgt
     4801 tttaagtgta attcggggta atgactccaa cttactgata gtgttttatg ttc
//
LOCUS       DAETPZ010000341         4816 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.guided.191, whole genome shotgun sequence.
ACCESSION   DAETPZ010000341 DAETPZ010000000
VERSION     DAETPZ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.guided.191"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..703
                     /locus_tag="K0W59_004519"
     CDS             <1..703
                     /locus_tag="K0W59_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBI3025184.1"
                     /translation="TRIKGLFAVGECSSVGLHGANRLGSNSLAELVVFGRLAGEQATE
                     RAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWAKIRDEMGLAMEEGCGIYRTPE
                     LMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIELSHGLNVAECMAHSAMARKES
                     RGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEYSDVKITTLPPAKRVYGGEADA
                     ADKAEAANNKEKANG"
     gene            696..1430
                     /gene="frdB"
                     /locus_tag="K0W59_004520"
     CDS             696..1430
                     /gene="frdB"
                     /locus_tag="K0W59_004520"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBI3025185.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDSMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            1441..1836
                     /gene="frdC"
                     /locus_tag="K0W59_004521"
     CDS             1441..1836
                     /gene="frdC"
                     /locus_tag="K0W59_004521"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBI3025186.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            1847..2206
                     /gene="frdD"
                     /locus_tag="K0W59_004522"
     CDS             1847..2206
                     /gene="frdD"
                     /locus_tag="K0W59_004522"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBI3025187.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            2269..3402
                     /locus_tag="K0W59_004523"
     CDS             2269..3402
                     /locus_tag="K0W59_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBI3025188.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            3491..4024
                     /gene="blc"
                     /locus_tag="K0W59_004524"
     CDS             3491..4024
                     /gene="blc"
                     /locus_tag="K0W59_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBI3025189.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(4021..4250)
                     /locus_tag="K0W59_004525"
                     /pseudo
     CDS             complement(4021..4250)
                     /locus_tag="K0W59_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(4426..4572)
                     /gene="ecnB"
                     /locus_tag="K0W59_004526"
     CDS             complement(4426..4572)
                     /gene="ecnB"
                     /locus_tag="K0W59_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBI3025190.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(4683..4808)
                     /gene="ecnA"
                     /locus_tag="K0W59_004527"
     CDS             complement(4683..4808)
                     /gene="ecnA"
                     /locus_tag="K0W59_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBI3025191.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
BASE COUNT         1202 a         1240 c         1270 g         1104 t
ORIGIN      
        1 aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc tgcacggtgc
       61 aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc tggccggtga
      121 acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa ttgaagcgca
      181 ggcagctggc gttgaacaac gtctgaaaga tctggttaac caggatggcg gcgaaaactg
      241 ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta tctaccgtac
      301 gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac gcttcaagcg
      361 cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca ccattgaact
      421 gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac gtaaagagtc
      481 ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg tcaactttct
      541 caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctggagt acagcgacgt
      601 gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg atgcagccga
      661 taaggcggaa gcagccaata ataaggagaa ggcgaatggc tgagatgaaa aacctgaaaa
      721 ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca ttctatgaag
      781 tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa gacaacctgg
      841 caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc tgcggcatga
      901 tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac accgacagta
      961 tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc gatatgaccc
     1021 acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc cgcaccgcgg
     1081 atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag ttctccggtt
     1141 gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac ccagagttca
     1201 tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc gaccacggta
     1261 agaaggagcg tatggcgcag ttgaacagcc agaacggtgt atggagctgt actttcgtgg
     1321 gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt cagcagggca
     1381 aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa ggagtgcaac
     1441 atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg gaaaaaattg
     1501 ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt gtggttcagc
     1561 attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg ggcgggattc
     1621 gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct gacggcagct
     1681 ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat cattgtaaaa
     1741 gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac tgtggttgcc
     1801 accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga ttaatccaaa
     1861 tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg gtatgtggag
     1921 cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac tggggctgtt
     1981 tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct tcattggtcg
     2041 cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc gtatgcacca
     2101 cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa tgggttttct acggtctggc
     2161 tgctatcctg acagttgtca cgctgattgg tgtcgttaca atctaacgca tcgccaatgt
     2221 aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat gttcaaaacg
     2281 acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc tcaacaaatc
     2341 aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat ccccggtatg
     2401 gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta tgcggacatc
     2461 gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt cagcaaaaca
     2521 tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt aagcgatccc
     2581 gcaacaaaat actggcctga acttaccgct aaacagtgga atgggatcac actattacat
     2641 cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt gaaatcctca
     2701 agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg aacacaacgt
     2761 ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc gtctggtttg
     2821 agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa ccatacgtgg
     2881 attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga aggtaaggca
     2941 gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc gaccattgaa
     3001 gatatggccc gctgggtgca aagcaattta cccccccttg atatcaatga gaaaacgctt
     3061 caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat gtatcagggc
     3121 ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa cggcagtgac
     3181 aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac tcctgcagta
     3241 cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta tgtcgcgttt
     3301 attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc caatccagag
     3361 agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat cgggtccgaa
     3421 ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa aaaccagtaa
     3481 ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat ttctggtcgt
     3541 tgcctgtagt tctcctacgc cgccgcgtgg cgtgaccgta gtgaataatt tcgacgccaa
     3601 acgttatctc ggtacctggt atgagattgc ccgttttgat caccgttttg aacgtggact
     3661 ggaaaaagtc accgcaacat acagcctgcg tgatgacggc ggcctgaatg tcattaataa
     3721 aggctataac cctgacagag gaatgtggca gcagagtgaa gggaaagcgt actttaccgg
     3781 cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt cctttctatg gcggttataa
     3841 cgttattgca ctcgatcggg aataccgcca tgcgctggtt tgcggcccgg accgcgacta
     3901 cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg agatgctggc
     3961 agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc agcccggtag
     4021 ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca gactcgccag
     4081 acgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg cgccgactgc
     4141 gccaataccc gtccacacgg catcaaacgg ctaaagccgt gggtatattt caggccaacg
     4201 gcccatacca cttccggcag accagcaata actaagataa tccaggacat atcaggctcc
     4261 ggaaatttgg ggccgtcccc ggtgaaaaga ggcgtttgca ggtcgtcctg caaagctgat
     4321 ggttgaacgg atattttgcc aggtggcagg ggaatttcaa ctattacctt tatgacgatc
     4381 gcagaaaaaa caaacggcac gacactgctg tcgtaccgtt attgcttatt gctgcgcttt
     4441 cgttgctgcg ccagaaatcg cgttaccgcc atcagaaatg tcttcaccaa cgccacgcgt
     4501 ggtgttgcag gcagttaata ctgttgaaag caccagaaca gaaaagatcg ctgcaattgt
     4561 cttcttcacc ataacgtctt ccttttaggt aatgttattt atgtttgcct atagcaaatt
     4621 aataatagac aacatcgcca aaaatgatgg aaatctgatg atttttcgga agagaagaaa
     4681 aattagctgg cagcgcgaga gatggagttg ccgagatgtt tgatgtcttc gccgaaaccg
     4741 caagcggtgt tacagcccgt gagcagcgtg ctggcaagca aaacaagaac gataaggcgt
     4801 ttcatcatct ctgacc
//
LOCUS       DAETPZ010000342         4520 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.guided.198, whole genome shotgun sequence.
ACCESSION   DAETPZ010000342 DAETPZ010000000
VERSION     DAETPZ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.guided.198"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            112..207
                     /locus_tag="K0W59_004528"
     CDS             112..207
                     /locus_tag="K0W59_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBI3025192.1"
                     /translation="MLVFQMRYQMRYVDKTSTVLKQTKKSDYADK"
     gene            333..1070
                     /gene="erm(B)"
                     /locus_tag="K0W59_004529"
     CDS             333..1070
                     /gene="erm(B)"
                     /locus_tag="K0W59_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBI3025193.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1015..1185
                     /locus_tag="K0W59_004530"
     CDS             1015..1185
                     /locus_tag="K0W59_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HBI3025194.1"
                     /translation="MSKYCLFLIVIYYLTGGNNSMSRFCKFGKLHVTKGNVDKLLGIL
                     LTASKELKRSLD"
     gene            1700..2149
                     /locus_tag="K0W59_004531"
     CDS             1700..2149
                     /locus_tag="K0W59_004531"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3025195.1"
                     /translation="MTTILSDYNKSILIELLNTNRQIIVVHGDDIDDYYTDSCYDIIF
                     IDYYDDDDFTYNGQEIWRGDSIYLVKSYQDEIQNRIEKGQELIFVNKENDISFSESIL
                     TYYHLFDYAGKTFEIDGQIIDEQTKVFAAPERRCACRTCWKVPARKT"
     gene            2110..2451
                     /locus_tag="K0W59_004532"
     CDS             2110..2451
                     /locus_tag="K0W59_004532"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3025196.1"
                     /translation="MSHVLEGASQEDLNLYRAEVERDQAYGNWRDFFLNKKGSVTSVS
                     GDANLDQIADVSYLLDTFFAKVTRTALQNAASIAGLMITTEAMVAEAPKKDEPAMPPG
                     GGMGGMGGMDF"
     gene            2678..4210
                     /locus_tag="K0W59_004533"
     CDS             2678..4210
                     /locus_tag="K0W59_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBI3025197.1"
                     /translation="MPRLATSRRQAAGCAPLPSAHTGSRYARHAPERTLLYALVEAHY
                     PDFIARIEAEGRSLPGYVREAFDAYLRCGVLEHGFLRVVCEHCRAERLVAFSCKKRGF
                     CPSCGARRMAESARHLVEEVFGPRPVRQWVLSFPYPLRFLFASKPEAIGPVLGIVQRV
                     IAGWLADQAGIDRASAQCGAVTLIQRFGSALNLNIHFHMLWLDGVYVEATELPRRELR
                     LHRARAPTTAQLTQLAAAIAHRVCRHLTRKGWLEGEGESAFLADSAAGDDSMDGLRMS
                     SITYRIATGRDAGCKVVTLQTLPGDAGSLEGEAGKVGGFSLHAGVAAEAHESHKLEKL
                     CRYITRPAISEKRLSIALQGRVRYQLKTPWRNGTTHVEWDPVDFIAKLAALVPPPRAH
                     LTRFHGVFAPNAALRAQLTPSGRGRRHDAAVEPADASANDAPRSPEEKRRSMSWAQRL
                     KRVFSIDVTACVHCGGTVRIVASIEEPAAIRAILGHFVKQGAREEAHYRPAARAPPVQ
                     AA"
BASE COUNT         1190 a         1162 c         1156 g         1012 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc aaattttgta taataggaat
       61 tgaagttaaa ttagatgcta aaaatttgta attaagaagg agggattcgt catgttggta
      121 ttccaaatgc gttatcaaat gcgttatgta gataaaacat ctactgtttt gaaacagact
      181 aaaaaaagtg attacgcaga taaataaata cgttagatta attcctacca gtgactaatc
      241 ttatgacttt ttaaacagat aactaaaatt acaaacaaat cgtttaactt ctgtatttgt
      301 ttatagatgt aatcacttca ggagtaaatt acatgaacaa aaatataaaa tattctcaaa
      361 actttttaac gagtgaaaaa gtactcaacc aaataataaa acaattgaat ttaaaagaaa
      421 ccgataccgt ttacgaaatt ggaacaggta aagggcattt aacgacgaaa ctggctaaaa
      481 taagtaaaca ggtaacgtct attgaattag acagtcatct attcaactta tcgtcagaaa
      541 aattaaaact gaatactcgt gtcactttaa ttcaccaaga tattctacag tttcaattcc
      601 ctaacaaaca gaggtataaa attgttggga atattcctta ccatttaagc acacaaatta
      661 ttaaaaaagt ggtttttgaa agccatgcgt ctgacatcta tctgattgtt gaagaaggat
      721 tctacaagcg taccttggat attcaccgaa cactagggtt gctcttgcac actcaagtct
      781 cgattcagca attgcttaag ctgccagcgg aatgctttca tcctaaacca agagtaaaca
      841 gtgtcttaat aaaacttacc cgccatacca cagatgttcc agataaatat tggaagctat
      901 atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt actaaaaatc
      961 agtttcatca agcaatgaaa cacgccaaag taaacaattt aagtaccgtt acttatgagc
     1021 aagtattgtc tatttttaat agttatctat tatttaacgg gaggaaataa ttctatgagt
     1081 cgcttttgta aatttggaaa gttacacgtt actaaaggga atgtagataa attattaggt
     1141 atactactga cagcttccaa ggagctaaag aggtccctag actagcaaga agtacacaag
     1201 aagccttaaa gattatagaa aagcttttcc atgaaatgct taacattatt taatgttaac
     1261 atgtggcttt gtggtaacta ccacgagtgg taacttgtaa catgaaaaaa gttacaagtt
     1321 acaagtggtt tatttacgaa cataaaggct aggaaagcac ttgataagta tatcaagtgc
     1381 tttttttccg ttcgatttta ctatccaaga taccgcttga cgtacctaat attttagtgt
     1441 gctaagggct ttgctttttc tggttatagt tcgttcaaaa aagaccgcaa gggtaaatgc
     1501 acgatcatag atcgcccttg cagcttttta atctctccac ttttttataa ccaagcaaac
     1561 caccaagcac actgaaaaaa taattaggaa ctaaagcgaa gtgcgtatct tggataaatc
     1621 ttcactcgat tttaaaagat taagcaccca gccaaagcgc ctaaaaaaat aattttttta
     1681 aagaaaggaa gtaatttaaa tgactacaat tttaagcgac tacaataaat ctatcctaat
     1741 tgaattacta aacaccaatc gccaaattat cgtcgttcat ggcgacgata ttgatgatta
     1801 ttacacagat tcttgctatg acattatctt tattgattac tatgatgatg atgatttcac
     1861 ttataacgga caggaaatat ggcgtggcga tagcatttat cttgtaaaaa gttatcaaga
     1921 tgaaattcaa aaccgtatag aaaaaggaca agagctgata tttgttaata aagaaaatga
     1981 tatatcattt tcagaatcta ttttaactta ttaccatcta tttgattatg ctggtaaaac
     2041 gtttgaaata gacggtcaaa tcattgatga acaaacaaaa gtttttgccg cacccgagcg
     2101 ccgctgcgca tgtcgcacgt gctggaaggt gccagccagg aagacctgaa cctttaccgc
     2161 gcggaagtgg agcgcgacca ggcctatggc aactggcgcg atttcttcct gaacaagaag
     2221 ggcagcgtca catccgtgag cggtgatgcc aacctcgacc agatcgcgga cgtgtcgtac
     2281 ctgctggata ccttctttgc caaggtcacc cgcaccgcgt tgcagaacgc cgcctccatc
     2341 gcgggcctga tgatcaccac cgaagccatg gtggccgagg ccccgaagaa ggacgagccg
     2401 gcgatgccgc cgggcggcgg catgggtggc atgggcggca tggatttctg atgcggttgg
     2461 cccggtcgtc agggaaccgg accgcgccag cgcggtccga tcccggcaac gacccgacat
     2521 caaggcccca aggacggggc cggagcccgg cagcgatgcc gggctttttg ttgtgcccgc
     2581 gccgcggcaa tgtctgacgc gaagatcaga acgcaccgat acgaacgtgc gaacacaggt
     2641 gcaaccctga gcagccgtcc ccgcaccgga gcgctgcgtg ccgcgcctcg ccacatcccg
     2701 gcggcaagcc gcgggatgcg cgccactgcc gtccgcccac accggttcgc ggtacgcgcg
     2761 ccacgcgccc gagcgcacgc tgctgtacgc gttggtagag gcgcactacc cggacttcat
     2821 tgcacggatc gaagcggagg gccgctcgct gcccgggtat gtccgcgagg cgttcgatgc
     2881 ctacctgcgt tgcggcgtgc tcgagcacgg cttcctgcgg gtggtgtgcg agcactgccg
     2941 tgcagagagg ctggtggcct tctcctgcaa gaagcgcggg ttctgcccga gttgcggcgc
     3001 gcgacgcatg gccgagagtg cgcggcacct ggtcgaggag gtgttcggcc cgcggcctgt
     3061 gcggcaatgg gtgctgagct ttccgtaccc cttgcgtttc ctgttcgcca gcaagccaga
     3121 agccattggc ccggtgctgg gcatcgtgca gcgcgtgatc gccggctggt tggccgatca
     3181 agccggcatc gaccgcgcca gcgcccagtg cggcgcggtg acgctgatcc agcgtttcgg
     3241 cagcgcgctg aacctgaaca tccacttcca catgctgtgg ctcgacggcg tgtacgtgga
     3301 agccaccgag ctgccgcggc gcgaactgcg cctgcaccgc gcccgtgcgc ccaccaccgc
     3361 gcagttgacc cagctggcag ctgccatcgc gcaccgggtg tgtcggcacc tgacgcgcaa
     3421 aggctggctc gaaggggagg gcgaatcggc cttcctggca gacagcgctg caggcgacga
     3481 cagcatggat gggctgcgga tgagttcgat cacctaccgc atcgccaccg gccgcgacgc
     3541 tggctgcaag gtcgtcacgc tgcaaacgct gcccggtgac gccggttcgc tggagggcga
     3601 agccggcaag gtcggcggct tctcactgca tgccggcgtg gcggccgaag cacacgaaag
     3661 ccacaagctg gaaaagctgt gccgctacat cacgcgcccg gcgatcagcg agaagcggct
     3721 gtcgatagcg ctccagggca gggtgcgtta ccagctcaag accccgtggc gcaatggcac
     3781 cacgcatgtg gaatgggatc cggtggattt catcgccaag ctggcggcgc tggtcccgcc
     3841 accgcgcgcc cacctgaccc gcttccacgg ggtgtttgcc ccgaacgccg ccctgcgcgc
     3901 acagctgacg ccatcggggc gcggcaggcg gcatgacgcc gctgtggagc cggcggacgc
     3961 aagcgcgaac gacgcgccgc gcagccccga ggagaagcgc cgttcgatga gctgggcgca
     4021 acgcctcaag cgggtctttt ccatcgacgt caccgcctgc gtccactgcg gtggcaccgt
     4081 gcggatcgtc gccagcatcg aggaacctgc cgccatccgc gccatccttg gccacttcgt
     4141 gaagcagggc gcgcgggaag aagcgcacta caggcccgca gcgcgcgcac cgccagtgca
     4201 agccgcgtga cgatctgccg gctgcacagc cgacggcgaa accggaatcc gagccgatgc
     4261 ggccacgatc cgcagggcgg cgctcggccc gctgtcggga atcagcgaag catggctgct
     4321 gacaacgccg ctgcgtggcc ccgcgatgcc gaaatcccac tcacagacgt ccgatccgtg
     4381 cccaaaacgg ggcttgcgcg accgccgcct acccagcaga ctgcccgaaa agggcgtttg
     4441 aacttcctat acgctgatag tgcattatct taaaattttg ggcactgttg caaagttagc
     4501 gatgaggcag ccttttgtct
//
LOCUS       DAETPZ010000343         1141 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH141
            SAMN15686854-rid12761883.guided.290, whole genome shotgun sequence.
ACCESSION   DAETPZ010000343 DAETPZ010000000
VERSION     DAETPZ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686854
            Sequence Read Archive: SRR12358877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097975.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:04:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,534
            CDSs (total)                      :: 4,440
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 446
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 446
            Pseudo Genes (frameshifted)       :: 212 of 446
            Pseudo Genes (incomplete)         :: 136 of 446
            Pseudo Genes (internal stop)      :: 146 of 446
            Pseudo Genes (multiple problems)  :: 46 of 446
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686854-rid12761883.guided.290"
                     /isolate="SH141"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            264..923
                     /locus_tag="K0W59_004534"
     CDS             264..923
                     /locus_tag="K0W59_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HBI3025198.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT          310 a          247 c          268 g          316 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     1081 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     1141 g
//
